LOCUS       DAJTRC010000001       123633 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.001, whole genome shotgun sequence.
ACCESSION   DAJTRC010000001 DAJTRC010000000
VERSION     DAJTRC010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 123633)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 123633)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..123633
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.001"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            231..1664
                     /gene="cscA"
                     /locus_tag="OO357_000001"
     CDS             231..1664
                     /gene="cscA"
                     /locus_tag="OO357_000001"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HCR5885107.1"
                     /translation="MTQSRLHAAQNALAKLHEHRGNTFYPHFHLAPPAGWMNDPNGLI
                     WFNDRYHAFYQHHPMSEHWGPMHWGHATSDDMIHWQHEPIALALGDDNDKDGCFSGSA
                     VDDNGVLSLIYTGHVWLDGAGNDDAIREVQCLATSRDGIHFEKQGVILTPPEGIMHFR
                     DPKVWREADTWWMVVGAKDPGNTGQILLYRGSSLREWAFDRVLAHADAGESYMWECPD
                     FFSLGDQHYLMFSPQGMNAEGYSYRNRFQSGVIPGMWSPGRLFAQSGHFTELDNGHDF
                     YAPQSFLAKDGRRIVIGWMDMWESPMPSKREGWAGCMTLARELSESNGKLLQRPVHEA
                     ESLRQQHQSVSPRRISNKYVLQENAQAVEIQLQWALKNSDAEHYGLQLGTGMRLYIDN
                     QSERLILWRYYPHENLDGYRSIPLPQRDTLALRIFIDTSSVEVFINDGEAVMSSRIYP
                     QPEERELSLYASHGVAVLQHGALWLLG"
     gene            complement(1672..2730)
                     /locus_tag="OO357_000002"
     CDS             complement(1672..2730)
                     /locus_tag="OO357_000002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5885108.1"
                     /translation="MDLASDVCFCYSARFTANGLGMASLKDVARLAGVSMMTVSRVMH
                     NAESVRPATRDRVLQAIQTLNYVPDLSARKMRAQGRKPSTLAVLAQDTATTPFSVDIL
                     LAIEQTASEFGWNSFLINIFSEDDAARAARQLLAHRPDGIIYTTMGLRHITLPESLYG
                     ENIVLANCVADDPALPSYIPDDYTAQYESTQHLLAAGYRQPLCFWLPESALATGYRRQ
                     GFEQAWRDAGRDLAEVKQFHMATGDDHYTDLASLLNAHFKSGKPDFDVLICGNDRAAF
                     VAYQVLLAKGVRIPQDVAVMGFDNLVGVGHLFLPPLTTIQLPHDIIGREAALHIIEGR
                     EGGRVTRIPCPLLIRCST"
     gene            2898..3116
                     /locus_tag="OO357_000003"
                     /pseudo
     CDS             2898..3116
                     /locus_tag="OO357_000003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416866.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine transporter DsdX"
     gene            3134..4462
                     /gene="dsdA"
                     /locus_tag="OO357_000004"
     CDS             3134..4462
                     /gene="dsdA"
                     /locus_tag="OO357_000004"
                     /EC_number="4.3.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine ammonia-lyase"
                     /protein_id="HCR5885109.1"
                     /translation="MENAKMNSLIAQYPLVKDLVALKETTWFNPSTTSLAEGLPYVGL
                     TEQDVQDAHARLSRFAPYLAKAFAETAATGGIIESELVAIPAMQKRLEKEYQQPISGQ
                     LLLKKDSHLPISGSIKARGGIYEVLAHAEKLALEAGLLTLDDDYSKLLSPEFKQFFSQ
                     YSIAVGSTGNLGLSIGIMSARIGFKVTVHMSADARAWKKAKLRSHGVTVVEYEQDYGV
                     AVEEGRKAAQSDPNCFFIDDENSRTLFLGYSVAGQRLKAQFAQQGRIVDADNPLFVYL
                     PCGVGGGPGGVAFGLKLAFGDHVHCFFAEPTHSPCMLLGVHTGLHDQISVQDIGIDNL
                     TAADGLAVGRASCFVGRAMERLLDGFYTLSDQTMYDMLGWLAQEEGIRLEPSALAGMA
                     GPQRVCASVSYQQMHGFSAEQLRNATHLVWATGGGMVPEEEMNQYLAKGR"
     gene            complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO357_000005"
     CDS             complement(4570..6108)
                     /gene="emrY"
                     /locus_tag="OO357_000005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrY"
                     /protein_id="HCR5885110.1"
                     /translation="MAITKSTPAPLTGGTLWCVTIALSLATFMQMLDSTISNVAIPTI
                     SGFLGASTDEGTWVITSFGVANAIAIPVTGRLAQRIGELRLFLLSVTFFSLSSLMCSL
                     STNLDVLIFFRVVQGLMAGPLIPLSQSLLLRNYPPEKRTFALALWSMTVIIAPICGPI
                     LGGYICDNFSWGWIFLINVPMGIIVLTLCLTLLKGRETETSPVKMNLPRLTLLVLGVG
                     GLQIMLDKGRDLDWFNSSTIIILTVVSVISLISLVIWESTSENPILDLSLFKSRNFTI
                     GIVSITCAYLFYSGAIVLMPQLLQKTMGYNAIWAGLAYAPIGIMPLLISPLIGRYGNK
                     IDMRVLVTFSFLMYAVCYYWRSVTFMPTIDFTGIILPQFFQGFAVACFFLPLTTISFS
                     GLPDNKFANASSMSNFFRTLSGSVGTSLTMTLWGRRESLHHSQLTATIDQFNPVFNSS
                     SQIMDKYYGSLSGVLNEINNEITQQSLSISANEIFRMAAIAFILLTVLVWFAKPPFTA
                     KGVG"
     gene            complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO357_000006"
     CDS             complement(6108..7271)
                     /gene="emrK"
                     /locus_tag="OO357_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrK"
                     /protein_id="HCR5885111.1"
                     /translation="MEQINSNKKQSNRKKYFSLLVIVLFIAFSGAYAYWSMELEDMIS
                     TDDAYVTGNADPISAQVSGSVTVVNHKDTNYVRQGDILVSLDKTDATIALNKAKNNLA
                     NIVRQTNKLYLQDKQYSAEVASARIQYQQSLEDYNRRVPLAKQGVISKETLEHTKDTL
                     ISSKAALNAAIQAYKANKALVMNTPLNRQPQVVEAADATKEAWLVLKRTDIRSPVTGY
                     IAQRSVQVGETVSSGQSLMAVVPARQMWVNANFKETQLTDVRIGQSVNIISDLYGENV
                     VFHGRVTGINMGTGNAFSLLPAQNATGNWIKIVQRVPVEVSLDPKELMEHPLRIGLSM
                     TATIDTKDEDIAEMPDLASTVTSMPAYTSKALVIDTSPIEKEISNIISHNGQL"
     gene            7687..8301
                     /gene="evgA"
                     /locus_tag="OO357_000007"
     CDS             7687..8301
                     /gene="evgA"
                     /locus_tag="OO357_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system DNA-binding response
                     regulator EvgA"
                     /protein_id="HCR5885112.1"
                     /translation="MNAIIIDDHPLAIAAIRNLLIKNDIEILAELTEGGSAVQRVETL
                     KPDIVIIDVDIPGVNGIQVLETLRKRQYSGIIIIVSAKNDHFYGKHCADAGANGFVSK
                     KEGMNNIIAAIEAAKNGYCYFPFSLNRFVGSLTSDQQKLDSLSKQEISVMRYILDGKD
                     NNDIAEKMFISNKTVSTYKSRLMEKLECKSLMDLYTFAQRNKIG"
     gene            8306..11899
                     /gene="evgS"
                     /locus_tag="OO357_000008"
     CDS             8306..11899
                     /gene="evgS"
                     /locus_tag="OO357_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-sensing system histidine kinase EvgS"
                     /protein_id="HCR5885113.1"
                     /translation="MKFLPYFFLLCCGLWSTISFADEDYIEYRGISSNNRVTLDPVRL
                     SNKELRWLASKKNLVIAVHKSQTATLLHTDSQQQVRGINADYLNLLKRALNIKLTLRE
                     YADHQKAMDALAEGEVDIVLSHLVTSPPLNNDIAATKPLIITFPALVTTLHDSMRPLT
                     SPKPVNIARVANYPPDEVIHQSFPKATIISFTNLYQALASVSAGHNDYFIGSNIITSS
                     MISRYFTHSLNVVKYYNSPRQYNFFLTRKESVILNEVLNRFVDALTNEVRYEVSQNWL
                     DTGNLAFLNKPLELTEHEKQWIKQHPNLKVLENPYSPPYSMTDENGSVRGVMGDILNI
                     ITLQTGLNFSPITVSHNIHAGTQLSPGGWDIIPGAIYSEDRENNVLFAEAFITTPYVF
                     VMQKAPDSEQTLKKGMKVAIPYYYELHSQLKEMYPEVEWIQVDNASAAFHKIKEGELD
                     ALVATQLNSRYMIDHYYPNELYHFLIPGVPNASLSFAFPRGEPELKDIINKALNAIPP
                     SEVLRLTEKWIKMPNVTIDTWDLYSEQFYIVTTLSVLLVGSSLLWGFYLLRSVRRRKV
                     IQGDLENQISFRKALSDSLPNPTYVVNWQGNVISHNSAFEHYFTADYYKNAMLPLENS
                     DSPFKDVFSNAHEVTAETKENRTIYTQVFEIDNGIEKRCINHWHTLCNLPASDNAVYI
                     CGWQDITETRDLINALEVEKNKAIKATVAKSQFLATMSHEIRTPISSIMGFLELLSGS
                     GLSKEQRVEAISLAYATGQSLLGLIGEILDVDKIESGNYQLQPQWVDIPTLVQNTCHS
                     FGAIAASKSIALSCSSTFPDHYLVKIDPQAFKQVLSNLLSNALKFTTEGAVKITTSLG
                     HIDDNHAVIKMTIMDSGSGLSQEEQQQLFKRYSQTSAGRQQTGSGLGLMICKELIKNM
                     QGDLSLESHPGIGTTFTITIPVEISQQVATVEAKAEQPITLPEKLSILIADDHPTNRL
                     LLKRQLNLLGYDVDEATDGVQALHKVSMQHYDLLITDVNMPNMDGFELTRKLREQNSS
                     LPIWGLTANAQANEREKGLSCGMNLCLFKPLTLDVLKTHLSQLHQVAHIAPQYRHLDI
                     EALKNNTANDLQLMQEILMTFQHETHKDLPAAFHALEAGDNRTFHQCIHRIHGAANIL
                     NLQKLINISHQLEITPASDDSKPEILQLLNSVKEHIAELDQEIAVFCQKND"
     gene            complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO357_000009"
     CDS             complement(11955..13100)
                     /gene="yfdE"
                     /locus_tag="OO357_000009"
                     /EC_number="2.8.3.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA:oxalate CoA-transferase"
                     /protein_id="HCR5885114.1"
                     /translation="MTNNESKGPFEGLLVIDMTHVLNGPFGTQLLYNMGARVIKVEPP
                     GHGDDTRTFGPYVDGQSLYYSFINHGKESVVLDLKNDHDKSIFINMLKQADVLAENFR
                     LGTMEKLGFSWERLQEINPRLIYASSSGFGHTGPLKDAPAYDTIIQAMSGIMMETGYP
                     DAPPVRVGTSLADLCGGVYLFSGIVSALYGREKSQRGAHVDIAMFDATLSFLEHGLMA
                     YIATGKSPQRLGNRHPYMAPFDVFDTQDKPITICCGNDKLFSALCQALELTELVNDPR
                     FSSNILRVQNQAILKQYIERTLKTQAAEVWLAKIHEVGVPVAPLLSVAEAINLPQTQA
                     RNMLIEAGGIMMPGNPIKISGCADPHVMPGAATLDQHGEQIRQEFSS"
     gene            complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO357_000010"
     CDS             complement(13174..14118)
                     /gene="yfdV"
                     /locus_tag="OO357_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter YfdV"
                     /protein_id="HCR5885115.1"
                     /translation="MLTFFIGDLLPIIVIMLLGYFSGRRETFSEDQARAFNKLVLNYA
                     LPAALFVSITRANREMIFADTRLTLVSLVVIVGCFFFSWFGCYKFFKRTHAEAAVCAL
                     IAGSPTIGFLGFAVLDPIYGDSVSTGLVVAIISIIVNAITIPIGLYLLNPSSGADGKK
                     NSNLSALISAAKEPVVWAPVLATILVLVGVKIPAAWDPTFNLIAKANSGVAVFAAGLT
                     LAAHKFEFSAEIAYNTFLKLILMPLALLLVGMACHLNSEHLQMMVLAGALPPAFSGII
                     IASRFNVYTRTGTASLAVSVLGFVVTAPLWIYVSRLVS"
     gene            complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO357_000011"
     CDS             complement(14188..15882)
                     /gene="oxc"
                     /locus_tag="OO357_000011"
                     /EC_number="4.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxalyl-CoA decarboxylase"
                     /protein_id="HCR5885116.1"
                     /translation="MSDQLQMTDGMHIIVEALKQNNIDTIYGVVGIPVTDMARHAQAE
                     GIRYIGFRHEQSAGYAAAASGFLTQKPGICLTVSAPGFLNGLTALANATVNGFPIIMI
                     SGSSDRAIVDLQQGDYEELDQMNAAKPYAKAAFRVNQPQDLGIALARAIRVSVSGRPG
                     GVYLDLPANVLAATMEKDEALTTIVKVENPSPALLPCPKSVTSAISLLAKAERPLIIL
                     GKGAAYSQADEQLREFIESAQIPFLPMSMAKGILEDTHPLSAAAARSFALANADVVML
                     VGARLNWLLAHGKKGWAADTQFIQLDIEPQEIDSNRPIAVPVVGDIASSMQGMLAELK
                     QNTFTTPLVWRDILNIHKQQNAQKMHEKLSTDTQPLNYFNALSAVRDVLRENQDIYLV
                     NEGANTLDNARNIIDMYKPRRRLDCGTWGVMGIGMGYAIGASVTSGSPVVAIEGDSAF
                     GFSGMEIETICRYNLPVTIVIFNNGGIYRGDGVDLSGAGAPSPTDLLHHARYDKLMDA
                     FRGVGYNVTTTDELRHALTTGIQSRKPTIINVVIDPAAGTESGHITKLNPKQVAGN"
     gene            complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO357_000012"
     CDS             complement(15936..17186)
                     /gene="frc"
                     /locus_tag="OO357_000012"
                     /EC_number="2.8.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyl-CoA transferase"
                     /protein_id="HCR5885117.1"
                     /translation="MSTPLQGIKVLDFTGVQSGPSCTQMLAWFGADVIKIERPGVGDV
                     TRHQLRDIPDIDALYFTMLNSNKRSIELNTKTAEGKEVMEKLIREADILVENFHPGAI
                     DHMGFTWEHIQEINPRLIFGSIKGFDECSPYVNVKAYENVAQAAGGAASTTGFWDGPP
                     LVSAAALGDSNTGMHLLIGLLAALLHREKTGRGQRVTMSMQDAVLNLCRVKLRDQQRL
                     DKLGYLEEYPQYPNGTFGDAVPRGGNAGGGGQPGWILKCKGWETDPNAYIYFTIQEQN
                     WENTCKAIGKPEWITDPAYSTAHARQPHIFDIFAEIEKYTVTIDKHEAVAYLTQFDIP
                     CAPVLSMKEISLDPSLRQSGSVVEVEQPLRGKYLTVGCPMKFSAFTPDIKAAPLLGEH
                     TAAVLQELGYSDDEIAAMKQNHAI"
     gene            complement(17699..18322)
                     /locus_tag="OO357_000013"
     CDS             complement(17699..18322)
                     /locus_tag="OO357_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdX family protein"
                     /protein_id="HCR5885118.1"
                     /translation="MKRLIMATMVTAILASSTVWAADNAPVAAQQQTQQTQKTAAAER
                     ISEQGLYAMRDVQVARLALFHGDPEKAKELTNEASALLSDDSTEWAKFAKPGKKTNLN
                     DDQYIVINASVGISESYVATPEKEAAIKIANEKMAKGDKKGAMEELRLAGVGVMENQY
                     LMPLKQTRNALADAQKLLDKKQYYEANLALKGAEDGIIVDSEALFVN"
     gene            18619..18894
                     /locus_tag="OO357_000014"
     CDS             18619..18894
                     /locus_tag="OO357_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885119.1"
                     /translation="MKVNLILFSLFLLVSIMACNVFAFSISGGVSERSYKETEKTSAM
                     TTTHSTKLQPSQAILFKMREDVPPLNLTEEITPTYPTKANYLIHPVR"
     gene            complement(18971..19213)
                     /locus_tag="OO357_000015"
     CDS             complement(18971..19213)
                     /locus_tag="OO357_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfdY family protein"
                     /protein_id="HCR5885120.1"
                     /translation="MIYLWMFLALCIVCVSGYIGQVLNVVSAVSSFFGMVILAALIYY
                     FTMWLTGGNELVTGIFMFLAPACGLMIRFMVGYGRR"
     gene            19566..20486
                     /gene="lpxP"
                     /locus_tag="OO357_000016"
     CDS             19566..20486
                     /gene="lpxP"
                     /locus_tag="OO357_000016"
                     /EC_number="2.3.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311285.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) palmitoleoyltransferase"
                     /protein_id="HCR5885121.1"
                     /translation="MFPQCKFSREFLHPRYWLTWFGLGVLWLWVQLPYPVLCFLGTRI
                     GAMARPFLKRRESIARKNLELCFPQHSAEEREKMIAENFRSLGMALVETGMAWFWPDS
                     RVRKWFDVEGLDNLKRAQMQNRGVMVVGVHFMSLELGGRVMGLCQPMMATYRPHNNQL
                     MEWVQTRGRMRSNKAMIGRNNLRGIVGALKKGEAVWFAPDQDYGRKGSSFAPFFAVEN
                     VATTNGTYVLSRLSGAAMLTVTMVRKADYSGYRLFITPEMEGYPTDENQAAAYMNKII
                     EKEIMRAPEQYLWIHRRFKTRPVGESSLYI"
     gene            20841..20912
                     /gene="ypdK"
                     /locus_tag="OO357_000017"
     CDS             20841..20912
                     /gene="ypdK"
                     /locus_tag="OO357_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_077777854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YpdK"
                     /protein_id="HCR5885122.1"
                     /translation="MKYFFMGISFMVIVWAGTFALMI"
     gene            complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO357_000018"
     CDS             complement(20977..22215)
                     /gene="alaC"
                     /locus_tag="OO357_000018"
                     /EC_number="2.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708249.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase"
                     /protein_id="HCR5885123.1"
                     /translation="MADTRPERRFTRIDRLPPYVFNITAELKMAARRRGEDIIDFSMG
                     NPDGATPPHIVEKLCTVAQRPDTHGYSTSRGIPRLRRAISRWYQDRYDVEIDPESEAI
                     VTIGSKEGLAHLMLATLDHGDTVLVPNPSYPIHIYGAVIAGAQVRSVPLVEGVDFFNE
                     LERAIRESYPKPKMMILGFPSNPTAQCVELEFFEKVVALAKCYDVLVVHDLAYADIVY
                     DGWKAPSIMQVPGARDVAVEFFTLSKSYNMAGWRIGFMVGNKTLVSALARIKSYHDYG
                     TFTPLQVAAIAALEGDQQCVRDIAEQYKRRRDVLVKGLHEAGWMVEMPKASMYVWAKI
                     PEPYAAMGSLEFAKKLLNEAKVCVSPGIGFGDYGDTHVRFALIENHDRIRQAIRGIKA
                     MFRADGLLPVSSKHIHENAE"
     gene            22592..24078
                     /locus_tag="OO357_000019"
                     /pseudo
     CDS             22592..24078
                     /locus_tag="OO357_000019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311287.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
     gene            24093..24827
                     /gene="ypdB"
                     /locus_tag="OO357_000020"
     CDS             24093..24827
                     /gene="ypdB"
                     /locus_tag="OO357_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator YpdB"
                     /protein_id="HCR5885124.1"
                     /translation="MKVIIVEDEFLAQQELSWLIKEHSQMEIVGTFDDGLDVLKFLQH
                     NRVDAIFLDINIPSLDGVLLAQNISQFAHKPFIVFITAWKEHAVEAFELEAFDYILKP
                     YQESRITGMLQKLEAAWQQQQTSSTPAATVTRENDTINLVKDERIIVTPINDIYYAEA
                     HEKMTFVYTRRESYVMPMNITEFCSKLPPSHFFRCHRSFCVNLNKIREIEPWFNNTYI
                     LRLKDLDFEVPVSRSKVKEFRQLMHL"
     gene            24840..25697
                     /locus_tag="OO357_000021"
                     /pseudo
     CDS             24840..25697
                     /locus_tag="OO357_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311289.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="OO357_000022"
     CDS             complement(25700..28195)
                     /gene="ptsP"
                     /locus_tag="OO357_000022"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5885125.1"
                     /translation="MLTIQFLCPLPNGLHARPAWELKEQCSQWQSEITFINHRQNAKA
                     DAKSSLALIGTGTLFNDSCSLNISGSDEEQARRVLEEYILVRFIDSDSVQPTQAELTA
                     HPLPRSLSRLNPDLLYGNVLASGVGVGTLTLLQSDSLDSYRAIPASAQDSTRLEHSLA
                     TLAEQLNQQLRERDGESKTILSAHLSLIQDDEFAGNIRRLMTEQHQGLGAAIISNMEQ
                     VCAKLSASASDYLRERVSDIRDISEQLLHITWPELKPRNNLVLEKPTILVAEDLTPSQ
                     FLSLDLKNLAGMILEKTGRTSHTLILARASAIPVLSGLPLDAIARYAGQPAVLDAQCG
                     VLAINPNDAVSGYYQVAQTLADKRQKQQAQAAAQLAYSRDNKRIDIAANIGTALEAPG
                     AFANGAEGVGLFRTEMLYMDRDSAPDEQEQFEAYQQVLLAAGDKPIIFRTMDIGGDKS
                     IPYLNIPQEENPFLGYRAVRIYPEFAGLFRTQLRAILRAASFGNAQLMIPMVHSLDQI
                     LWVKGEIQKAIVELKRDGLRHAETITLGIMVEVPSVCYIIDHFCDEVDFFSIGSNDMT
                     QYLYAVDRNNPRVSPLYNPITPSFLRMLQQIVTTAHQRGKWVGICGELGGESRYLPLL
                     LGLGLDELSMSSPRIPAVKSQLRQLDSEACRELARQACECRSAQEIEALLTAFTPEED
                     VRPLLALENIFVDQDFSNKEQAIQFLCGNLGVNGRTEHPFELEEDVWQREEIVTTGVG
                     FGVAIPHTKSQWIRHSSISIARLAKPVDWQSEMGEVELVIMLTLGANEGMNHVKVFSQ
                     LARKLVNKNFRQSLFAAQDAQSILTLLETELTF"
     gene            complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="OO357_000023"
     CDS             complement(28220..29257)
                     /gene="ypdE"
                     /locus_tag="OO357_000023"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5885126.1"
                     /translation="MDLSLLKALSEADAIASSEQEVRQILLEEAARLQKEVRFDGLGS
                     VLIRLNESTGPKVMICAHMDEVGFMVRSISREGAIDVLPVGNVRMAARQLQPVRITTR
                     EECKIPGLLDGDRQGNDVSAMRVDIGARTYDEVMQAGIRPGDRVTFDTTFQVLPHQRV
                     MGKAFDDRLSCYLLVTLLRELHDAELPAEVWLVASSSEEVGLRGGQTATRAVSPDVAI
                     VLDTACWAKNFDYGAANHRQIGNGPMLVLSDKSLIAPPKLTAWIETVAAEIGVPLQAD
                     MFSNGGTDGGAVHLTGTGVPTLVMGPATRHGHCAASIADCRDILQMEQLLSALIQRLT
                     RETVVQLTDFR"
     gene            complement(29257..30340)
                     /gene="ypdF"
                     /locus_tag="OO357_000024"
                     /pseudo
     CDS             complement(29257..30340)
                     /gene="ypdF"
                     /locus_tag="OO357_000024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416886.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
     gene            complement(30355..31602)
                     /locus_tag="OO357_000025"
     CDS             complement(30355..31602)
                     /locus_tag="OO357_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HCR5885127.1"
                     /translation="MAIKKRSATVVPGASGAAAAVKNPQASKSSFWGELPQHVMSGIS
                     RMVPTLIMGGVILAFSQLIAYSWLKIPAEIGIMDALNSGKFSGFDLSLLKFAWLSQSF
                     GGVLFGFAIPMFAAFVANSIGGKLAFPAGFIGGLMSTQPTQLLNFDPSTMQWATSSPV
                     PSTFIGALIISIVAGYLVKWMNQKIQLPDFLLAFKTTFLLPILSAIFVMLAMYYVITP
                     FGGWINGGIRTVLTAAGEKGALMYAMGIAAATAIDLGGPINKAAGFVAFSFTTDHVLP
                     VTARSIAIVIPPIGLGLATIIDRRLTGKRLFNAQLYPQGKTAMFLAFMGISEGAIPFA
                     LESPITAIPSYMVGAIVGSTAAVWLGAVQWFPESAIWAWPLVTNLGVYMAGIALGAII
                     TALMVVFLRLMMFRKGKLLIDSL"
     gene            complement(31624..31950)
                     /locus_tag="OO357_000026"
     CDS             complement(31624..31950)
                     /locus_tag="OO357_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708254.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-like phosphotransferase enzyme IIB
                     component 1"
                     /protein_id="HCR5885128.1"
                     /translation="MSKKLIALCACPMGLAHTFMAAQVLEEAAVEAGYEVKIETQGAD
                     GIQNRLTAQDIAEATIIIHSVAVTPEDNERFESRDVYEITLQDAIKNAAGIIKEIEEM
                     IASEQQ"
     gene            complement(32169..33134)
                     /gene="glk"
                     /locus_tag="OO357_000027"
     CDS             complement(32169..33134)
                     /gene="glk"
                     /locus_tag="OO357_000027"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HCR5885129.1"
                     /translation="MTKYALVGDVGGTNARLALCDIASGEISQAKTYSGLDYPSLEAV
                     IRVYLEEHKVEVKDGCIAIACPITGDWVAMTNHTWAFSIAEMKKNLGFSHLEIINDFT
                     AVSMAIPMLKKEHLIQFGGAEPVEGKPIAVYGAGTGLGVAHLVHVDKRWVSLPGEGGH
                     VDFAPNSEEEAIILEILRAEIGHVSAERVLSGPGLVNLYRAIVKADNRLPENLKPKDI
                     TERALADSCTDCRRALSLFCVIMGRFGGNLALNLGTFGGVFIAGGIVPRFLEFFKASG
                     FRAAFEDKGRFKEYVHDIPVYLIVHDYPGLLGSGAHLRQTLGHIL"
     gene            33338..34594
                     /locus_tag="OO357_000028"
     CDS             33338..34594
                     /locus_tag="OO357_000028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel protein"
                     /protein_id="HCR5885130.1"
                     /translation="MLHPRARTMLLLSLPAVAIGIASSLILIVVMKIASVLQNLLWQR
                     LPGTLGIAQDSPLWIIGVLTLTGIAVGLVIRFSQGHAGPDPACEPLIGAPVPPSALPR
                     LIVALILGLAGGVSLGPEHPIMTVNIALAVAIGARLLPRVNRMEWTILASAGTIGALF
                     GTPVAAALIFSQTLNGSSEVPLWDRLFAPLMAAAAGALTTGLFFHPHFSLPIAHYGQM
                     EMTDILSGAIVAAIAIAAGMVAVWCLPRLHAMMNQMKNPVLVLGIGGFILGILGVIGG
                     PVSLFKGLDEMQQMVANQAFSTSDYFLLAVIKLAALVVAAASGFRGGRIFPAVFVGVA
                     LGLMLHEHVPAVPAAITVSCAILGIVLVVTRDGWLSLFMAAVVVPNTTLLPLLCIVML
                     PAWLLLAGKPMMMVNRPKQQPPHDNV"
     gene            34709..35035
                     /locus_tag="OO357_000029"
     CDS             34709..35035
                     /locus_tag="OO357_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5885131.1"
                     /translation="MFRSLFLAAALMAFTPLAANAGEITLLPSIKLQIGDRDHYGNYW
                     DGGHWRDRDYWHRNYEWRKNRWWRHDNGYHRGWDKRKAYERGYREGWRDRDDHRGKGR
                     GHGHRH"
     gene            complement(35176..36414)
                     /locus_tag="OO357_000030"
     CDS             complement(35176..36414)
                     /locus_tag="OO357_000030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HCR5885132.1"
                     /translation="MTNYRVESSSGRAARKMRLALMGPAFIAAIGYIDPGNFATNIQA
                     GASFGYQLLWVVVWANLMAMLIQILSAKLGIATGKNLAEQIRDHYPRPVVWFYWVQAE
                     IIAMATDLAEFIGAAIGFKLILGVSLLQGAVLTGIATFLILMLQRRGQKPLEKVIGGL
                     LLFVAAAYIVELIFSQPNLAQLGKGMVIPSLPTSEAVFLAAGVLGATIMPHVIYLHSS
                     LTQHLHGGSRQQRYSATKWDVAIAMTIAGFVNLVMMATAAAAFHFSGHTGVADLDEAY
                     LTLQPLLSHAAATVFGLSLVAAGLSSTVVGTLAGQVVMQGFIRFHIPLWVRRTVTMLP
                     SFIVILMGLDPTRILVMSQVLLSFGIALALVPLLIFTSDSKLMGDLVNSKRVKQTGWV
                     IVVLVVALNIWLLVGTALGL"
     gene            36750..37952
                     /gene="nupC"
                     /locus_tag="OO357_000031"
     CDS             36750..37952
                     /gene="nupC"
                     /locus_tag="OO357_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708259.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupC"
                     /protein_id="HCR5885133.1"
                     /translation="MDRVLHFVLALAVVVILALLVSSDRKKIRIRYVIQLLVIEVLLA
                     WFFLNSDVGLGFVKGFSEMFEKLLGFANEGTNFVFGSMNDQGLAFFFLKVLCPIVFIS
                     ALIGILQHIRVLPVIIRAIGFLLSKVNGMGKLESFNAVSSLILGQSENFIAYKDILGK
                     ISRNRMYTMAATAMSTVSMSIVGAYMTMLEPKYVVAALVLNMFSTFIVLSLINPYRVD
                     ASEENIQMSNLHEGQSFFEMLGEYILAGFKVAIIVAAMLIGFIALIAALNALFATVTG
                     WFGYSISFQGILGYIFYPIAWVMGVPSSEALQVGSIMATKLVSNEFVAMMDLQKIAST
                     LSPRAEGIISVFLVSFANFSSIGIIAGAVKGLNEEQGNVVSRFGLKLVYGSTLVSVLS
                     ASIAALVL"
     gene            complement(38002..40114)
                     /locus_tag="OO357_000032"
                     /pseudo
     CDS             complement(38002..40114)
                     /locus_tag="OO357_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416896.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing phosphodiesterase"
     gene            complement(40322..40397)
                     /locus_tag="OO357_000033"
     tRNA            complement(40322..40397)
                     /locus_tag="OO357_000033"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(40362..40364),aa:Ala,seq:ggc)
     gene            40618..40977
                     /locus_tag="OO357_000034"
     CDS             40618..40977
                     /locus_tag="OO357_000034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5885134.1"
                     /translation="MFKERMTPDELARLTGYSRQTINKWVRKEGWTTSPKPGVQGGKA
                     RLVHVNEQVREYIRNAERPEGQGEAPALSGDAPLEVLLVTLAKEMTPVEQKQFTSLLL
                     REGIIGLLQRLGIRDSK"
     gene            40979..41371
                     /locus_tag="OO357_000035"
     CDS             40979..41371
                     /locus_tag="OO357_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416898.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding transcriptional regulator"
                     /protein_id="HCR5885135.1"
                     /translation="MKRLRNKMTTEELAECLGVAKQTVNRWTREKGWKTEKFPGVKGG
                     RARLILVDTQVCEFIQNTPAFHNTPMLMEAEERIAEYAPGARAPAYRQIINAIDNMTD
                     IEQEKVAQFLSREGIRNFLARLDIDESA"
     gene            complement(41423..42838)
                     /gene="gltX"
                     /locus_tag="OO357_000036"
     CDS             complement(41423..42838)
                     /gene="gltX"
                     /locus_tag="OO357_000036"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HCR5885136.1"
                     /translation="MKIKTRFAPSPTGYLHVGGARTALYSWLFARNHGGEFVLRIEDT
                     DLERSTPEAIEAIMDGMNWLSLEWDEGPYYQTKRFDRYNAVIDQMLEEGTAYKCYCSK
                     ERLEALREEQMAKGEKPRYDGRCRHSHEHHADDEPCVVRFANPQEGSVVFDDQIRGPI
                     EFSNQELDDLIIRRTDGSPTYNFCVVVDDWDMEITHVIRGEDHINNTPRQINILKALK
                     APVPVYAHVSMINGDDGKKLSKRHGAVSVMQYRDDGYLPEALLNYLVRLGWSHGDQEI
                     FTREEMIKYFTLNAVSKSASAFNTDKLLWLNHHYINALPPEYVATHLQWHIEQENIDT
                     RNGPQLADLVKLLGERCKTLKEMAQSCRYFYEDFAEFDADAAKKHLRPVARQPLEVVR
                     DKLAAITDWTAENVHHAIQATADELEVGMGKVGMPLRVAVTGAGQSPALDVTVHAIGK
                     TRSIERINKALDFIAERENQQ"
     gene            43097..43172
                     /locus_tag="OO357_000037"
     tRNA            43097..43172
                     /locus_tag="OO357_000037"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43130..43132,aa:Val,seq:tac)
     gene            43217..43292
                     /locus_tag="OO357_000038"
     tRNA            43217..43292
                     /locus_tag="OO357_000038"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43250..43252,aa:Val,seq:tac)
     gene            43297..43372
                     /locus_tag="OO357_000039"
     tRNA            43297..43372
                     /locus_tag="OO357_000039"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43330..43332,aa:Lys,seq:ttt)
     gene            43403..43528
                     /locus_tag="OO357_000040"
     ncRNA           43403..43528
                     /ncRNA_class="other"
                     /locus_tag="OO357_000040"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            43648..43989
                     /locus_tag="OO357_000041"
     CDS             43648..43989
                     /locus_tag="OO357_000041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FlxA-like family protein"
                     /protein_id="HCR5885137.1"
                     /translation="MATISSTSIPSIQTQSSNRASQGSDVASQIARISQQIIKLTQQI
                     KEIVDTSGSAEDKQKQAELIQQQITLLETQLAQLQKQQAEKAQEKEQRLSLNVSLLNP
                     VEKTTHIDIYI"
     gene            complement(43980..44906)
                     /locus_tag="OO357_000042"
     CDS             complement(43980..44906)
                     /locus_tag="OO357_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5885138.1"
                     /translation="MNYSLKQLKVFVTVAQEKSFSRAGERIGLSQSAVSHSVKELENH
                     TGVRLLDRTTREVVLTDAGQQLALRLERLLDELNSTLRDTGRMGQQLSGKVRVAASQT
                     ISAHLIPQCIAESHRRYPDIQFVLHDRPQQWVMESIRQGDVDFGIVIDPGPVGDLQCE
                     AILSEPFFLLCHRDSALAVEDYVPWQALQGAKLVLQDYASGSRPLIDAALARNGIQAN
                     IVQEIGHPATLFPMVAAGIGISILPALALPLPEGSPLVVKRITPVVERQLMLVRRKNR
                     SLSTAAEALWDVVRDQGNALMAGREGDPLYQI"
     gene            44996..45994
                     /locus_tag="OO357_000043"
     CDS             44996..45994
                     /locus_tag="OO357_000043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter"
                     /protein_id="HCR5885139.1"
                     /translation="MKLFRILDPFTLTLITVVLLASFFPARGDFVPFFENLTTAAIAL
                     LFFMHGAKLSREAIIAGGGHWRLHLWVMCSTFVLFPILGVLFAWWKPVNVDPMLYSGF
                     LYLCILPATVQSAIAFTSMAGGNVAAAVCSASASSLLGIFLSPLLVGLVMNVHGARGS
                     LEQVGKIMLQLLLPFVLGHLSRPWIGDWVSRNKKWIAKTDQTSILLVVYTAFSEAVVN
                     GIWHKVGWGSLLFIVVVSCVLLAIVIVVNVFMARRLSFNKADEITIVFCGSKKSLANG
                     IPMANILFPTSVIGMMVLPLMIFHQIQLMVCAVLARRYKRQTEQLQAQQESSADKA"
     gene            complement(45991..46209)
                     /locus_tag="OO357_000044"
     CDS             complement(45991..46209)
                     /locus_tag="OO357_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3820 family protein"
                     /protein_id="HCR5885140.1"
                     /translation="MEKEQLIEIANTIMPFGKYKGRRLIDLPEEYLLWFARKDEFPAG
                     KLGELMQITLLIKTEGLTQLVQPLKRPL"
     gene            complement(46211..48226)
                     /gene="ligA"
                     /locus_tag="OO357_000045"
     CDS             complement(46211..48226)
                     /gene="ligA"
                     /locus_tag="OO357_000045"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HCR5885141.1"
                     /translation="MESIEQQLTELRTTLCHHEYLYHVMDAPEIPDAEYDRLMRELRE
                     LETKHPELITPDSPTQRVGAAPLAAFSQIRHEVPMLSLDNVFDEESFLAFNKRVQDRL
                     KNNEKVTWCCELKLDGLAVSILYENGVLVSAATRGDGTTGEDITSNVRTIRAIPLKLH
                     GENIPARLEVRGEVFLPQAGFEKINEDARRTGGKVFANPRNAAAGSLRQLDPRITAKR
                     PLTFFCYGVGVLEGGELPDTHLGRLLQFKKWGVPVSDRVTLCESAEEVLAFYHKVEED
                     RPTLGFDIDGVVIKVNSLEQQEQLGFVARAPRWAVAFKFPAQEQMTFVRDVEFQVGRT
                     GAITPVARLEPVHVAGVLVSNATLHNADEIERLGLRIGDKVVIRRAGDVIPQVVNVVL
                     SERPEDTREVVFPTHCPVCGSDVERVEGEAVARCTGGLICGAQRKESLKHFVSRRAMD
                     VDGMGDKIIDQLVEKEYVHTPADLFKLTAGKLTGLERMGPKLAQNVVNALEKAKETTF
                     ARFLYALGIREVGEATAAGLAAYFGTLEALEAASIEELQKVPDVGIVVASHVHNFFAE
                     ESNRNVISELLAEGVHWPAPIVINAEEIDSPFAGKTVVLTGSLSQMSRDDAKARLVEL
                     GAKVAGSVSKKTDLVIAGEAAGSKLAKAQELGIEVIDEAEMLRLLGS"
     gene            complement(48297..49283)
                     /gene="zipA"
                     /locus_tag="OO357_000046"
     CDS             complement(48297..49283)
                     /gene="zipA"
                     /locus_tag="OO357_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZipA"
                     /protein_id="HCR5885142.1"
                     /translation="MMQDLRLILIIVGAIAIIALLVHGFWTSRKERSSMFRDRPLKRM
                     KSKRDDDSYDEDVEDDEGVGEVRVHRVNHAPANAQEHEAARPSPQHQYLPPYASAQPR
                     QPVQQPPEAQVPPQHAPRPAQPVQQPAYQPQPEQPLQQPVSPQVAPAPQPVHSAPQPA
                     QQAFQPAEPVAAPQPEPVAEPAPVMDKPKRKEAVIIMNVAAHHGSELNGELLLNSIQQ
                     AGFIFGDMNIYHRHLSPDGSGPALFSLANMVKPGTFDPEMKDFTTPGVTIFMQVPSYG
                     DELQNFKLMLQSAQHIADEVGGVVLDDQRRMMTPQKLREYQDIIREVKDANA"
     gene            49513..50274
                     /gene="cysZ"
                     /locus_tag="OO357_000047"
     CDS             49513..50274
                     /gene="cysZ"
                     /locus_tag="OO357_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter CysZ"
                     /protein_id="HCR5885143.1"
                     /translation="MVSSFTSAPRSGFYYFAQGWKLVSQPGIRRFVILPLLVNILLMG
                     GAFWWLFTQLDVWIPTFMSYVPDWLQWLSYLLWPLAVISVLLVFGYFFSTIANWIAAP
                     FNGLLAEQLEARLTGATPPDTGIFGIMKDVPRIMKREWQKFVWYLPRAIVLLILYFIP
                     GIGQTVAPVLWFLFSAWMLAIQYCDYPFDNHKVPFKEMRTALRTRKITNMQFGALTSL
                     FTMIPLLNLFIMPVAVCGATAMWVDCYRDKHAMWR"
     gene            50459..51430
                     /gene="cysK"
                     /locus_tag="OO357_000048"
     CDS             50459..51430
                     /gene="cysK"
                     /locus_tag="OO357_000048"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000034402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HCR5885144.1"
                     /translation="MSKIFEDNSLTIGHTPLVRLNRIGNGRILAKVESRNPSFSVKCR
                     IGANMIWDAEKRGVLKPGVELVEPTSGNTGIALAYVAAARGYKLTLTMPETMSIERRK
                     LLKALGANLVLTEGAKGMKGAIQKAEEIVASNPEKYLLLQQFSNPANPEIHEKTTGPE
                     IWEDTDGQVDVFIAGVGTGGTLTGVSRYIKGTKGKTDLISVAVEPTDSPVIAQVLAGE
                     EIKPGPHKIQGIGAGFIPANLDLKLVDKVIGITNEEAISTARRLMEEEGILAGISSGA
                     AVAAALKLQEDESFTNKNIVVILPSSGERYLSTALFADLFTEKELQQ"
     gene            51814..52071
                     /gene="ptsH"
                     /locus_tag="OO357_000049"
     CDS             51814..52071
                     /gene="ptsH"
                     /locus_tag="OO357_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003038086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein Hpr"
                     /protein_id="HCR5885145.1"
                     /translation="MFQQEVTITAPNGLHTRPAAQFVKEAKGFTSEITVTSNGKSASA
                     KSLFKLQTLGLTQGTVVTISAEGEDEQKAVEHLVKLMAELE"
     gene            52116..53843
                     /gene="ptsI"
                     /locus_tag="OO357_000050"
     CDS             52116..53843
                     /gene="ptsI"
                     /locus_tag="OO357_000050"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate-protein phosphotransferase
                     PtsI"
                     /protein_id="HCR5885146.1"
                     /translation="MISGILASPGIAFGKALLLKEDEIVIDRKKISADQVDQEVERFL
                     SGRAKASAQLETIKTKAGETFGEEKEAIFEGHIMLLEDEELEQEIIALIKDKHMTADA
                     AAHEVIEGQASALEELDDEYLKERAADVRDIGKRLLRNILGLKIIDLSAIQDEVILVA
                     ADLTPSETAQLNLKKVLGFITDAGGRTSHTSIMARSLELPAIVGTGSVTSQVKNDDYL
                     ILDAVNNQVYVNPTNEVIDKMRAVQEQVASEKAELAKLKDLPAITLDGHQVEVCANIG
                     TVRDVEGAERNGAEGVGLYRTEFLFMDRDALPTEEEQFAAYKAVAEACGSQAVIVRTM
                     DIGGDKELPYMNFPKEENPFLGWRAIRIAMDRKEILRDQLRAILRASAFGKLRIMFPM
                     IISVEEVRALRKEIEIYKQELRDEGKAFDESIEIGVMVETPAAATIARHLAKEVDFFS
                     IGTNDLTQYTLAVDRGNDMISHLYQPMSPSVLNLIKQVIDASHAEGKWTGMCGELAGD
                     ERATLLLLGMGLDEFSMSAISIPRIKKIIRNTNFEDAKVLAEQALAQPTTDELMTLVN
                     KFIEEKTIC"
     gene            53884..54393
                     /gene="crr"
                     /locus_tag="OO357_000051"
     CDS             53884..54393
                     /gene="crr"
                     /locus_tag="OO357_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015833547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HCR5885147.1"
                     /translation="MGLFDKLKSLVSDDKKDTGTIEIIAPLSGEIVNIEDVPDVVFAE
                     KIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIESDSGVELFVHFGIDTVELKGE
                     GFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVISNMDEIKELIKLSGSVTVG
                     ETPVIRIKK"
     gene            complement(54435..55286)
                     /gene="pdxK"
                     /locus_tag="OO357_000052"
                     /gene_synonym="thiJ"
     CDS             complement(54435..55286)
                     /gene="pdxK"
                     /locus_tag="OO357_000052"
                     /gene_synonym="thiJ"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine/pyridoxal/pyridoxamine kinase"
                     /protein_id="HCR5885148.1"
                     /translation="MSSLLLFNDKSRALQADIVAVQSQVVYGSVGNSIAVPAIKQNGL
                     NVFAVPTVLLSNTPHYDTFYGGAIPDEWFSGYLRALQERDALRQLRAVTTGYMGTASQ
                     IKILAEWLTALRKDHPDLLIMVDPVIGDIDSGIYVKPDLPEAYRQYLLPLAQGITPNI
                     FELEILTGKNCRDLDSAIAAAKSLLSDTLKWVVITSASGNEENQEMQVVVVSADSVNV
                     ISHSRVKTDLKGTGDLFCAQLISGLLKGKALNDAVHRAGLRVLEVMRYTQQHESDELI
                     LPPLAEA"
     gene            55391..55765
                     /locus_tag="OO357_000053"
     CDS             55391..55765
                     /locus_tag="OO357_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfeK family protein"
                     /protein_id="HCR5885149.1"
                     /translation="MKKIICLAITLLMTLPAYAKLTVHEEARINAMLEGLAQKKDLIF
                     VRNGDEHTCDEAVSHLRLKLGNTRNRIDTAEQFIDKVASSSSITGKPYIVKIPGKSDE
                     NAQPFLHALIAQTDKTVPAEGN"
     gene            55798..56521
                     /locus_tag="OO357_000054"
                     /pseudo
     CDS             55798..56521
                     /locus_tag="OO357_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416915.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
     gene            complement(56725..57636)
                     /gene="cysM"
                     /locus_tag="OO357_000055"
     CDS             complement(56725..57636)
                     /gene="cysM"
                     /locus_tag="OO357_000055"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708275.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase B"
                     /protein_id="HCR5885150.1"
                     /translation="MSTLEQTIGNTPLVKLQRMGPDNGSEVWLKLEGNNPAGSVKDRA
                     ALSMIVEAEKRGEIKPGDVLIEATSGNTGIALAMIAALKGYRMKLLMPDNMSQERRAA
                     MRAYGAELILVTKEQGMEGARDLALEMANRGEGKLLDQFNNPDNPYAHYTTTGPEIWQ
                     QTGGRITHFVSSMGTTGTITGVSCFMREQSKPVTIVGLQPEEGSSIPGIRRWPAEYLP
                     GIFNAALVDEVLDIHQRDAENTMRELAVREGIFCGVSSGGAVAGALRVAKANPGAVVV
                     AIICDRGDRYLSTGVFGEEHFSQGAGI"
     gene            complement(57770..58867)
                     /gene="cysA"
                     /locus_tag="OO357_000056"
     CDS             complement(57770..58867)
                     /gene="cysA"
                     /locus_tag="OO357_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter ATP-binding
                     protein CysA"
                     /protein_id="HCR5885151.1"
                     /translation="MSIEIANIKKSFGRTQVLNDISLDIPSGQMVALLGPSGSGKTTL
                     LRIIAGLEHQTSGHIRFHGTDVSRLHARDRKVGFVFQHYALFRHMTVFDNITFGLTVL
                     PRRERPNAAAIKAKVTKLLEMVQLAHLADRYPAQLSGGQKQRVALARALAVEPQILLL
                     DEPFGALDAQVRKELRRWLRQLHEELKFTSVFVTHDQEEATEVADRVVVMSQGNIEQA
                     DAPNQVWREPATRFVLEFMGEVNRLQGTIRGGQFHVGAHRWPLGYTPAYQGPVDLFLR
                     PWEVDISRRTSLDSPLPVQVLEASPKGHYTQLVVQPLGWYNEPLTVVMHGDDAPQHGE
                     RLFVGLQHARLYNGDERIETRDEELALAQSA"
     gene            complement(58857..59732)
                     /gene="cysW"
                     /locus_tag="OO357_000057"
     CDS             complement(58857..59732)
                     /gene="cysW"
                     /locus_tag="OO357_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysW"
                     /protein_id="HCR5885152.1"
                     /translation="MAEVTQLKRYDARPINWGKWFLIGIGMLVSAFILLVPMIYIFVQ
                     AFSKGLMPVLQNLADPDMLHAIWLTVMIALIAVPVNLVFGILLAWLVTRFNFPGRQLL
                     LTLLDIPFAVSPVVAGLVYLLFYGSNGPLGGWLDEHNLQIMFSWPGMVLVTIFVTCPF
                     VVRELVPVMLSQGSQEDEAAILLGASGWQMFRRVTLPNIRWALLYGVVLTNARAIGEF
                     GAVSVVSGSIRGETLSLPLQIELLEQDYNTVGSFTAAALLTLMAIITLFLKSMLQWRL
                     ENQEKRAQQEEHHEH"
     gene            complement(59732..60565)
                     /gene="cysT"
                     /locus_tag="OO357_000058"
     CDS             complement(59732..60565)
                     /gene="cysT"
                     /locus_tag="OO357_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000458418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter permease
                     CysT"
                     /protein_id="HCR5885153.1"
                     /translation="MFAVSSRRVLPGFTLSLGTSLLFVCLILLLPLSALVMQLSEMSW
                     AQYWEVITNPQVVAAYKVTLLSAFVASIFNGVFGLLMAWILTRYRFPGRTLLDALMDL
                     PFALPTAVAGLTLASLFSVNGFYGEWLAKFDIKVTYTWLGIAVAMAFTSIPFVVRTVQ
                     PVLEELGPEYEEAAETLGATRWQSFCKVVLPELSPALVAGVALSFTRSLGEFGAVIFI
                     AGNIAWKTEVTSLMIFVRLQEFDYPAASAIASVILAASLLLLFSINTLQSRFGRRVVG
                     H"
     gene            complement(60565..61581)
                     /gene="cysP"
                     /locus_tag="OO357_000059"
     CDS             complement(60565..61581)
                     /gene="cysP"
                     /locus_tag="OO357_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate/sulfate ABC transporter
                     substrate-binding protein CysP"
                     /protein_id="HCR5885154.1"
                     /translation="MAVNLLKKNSLALVASLLLAGHVQATELLNSSYDVSRELFAALN
                     PPFEQQWAKDNGGDKLTIKQSHAGSSKQALAILQGLKADVVTYNQVTDVQILHDKGKL
                     IPADWQSRLPNNSSPFYSTMGFLVRKGNPKNIHDWNDLVRSDVKLIFPNPKTSGNARY
                     TYLAAWGAADKADGGDKAKTEQFMTQFLKNVEVFDTGGRGATTTFAERGLGDVLISFE
                     SEVNNIRKQYEAQGFEVVIPKTNILAEFPVAWVDKNVQANGTEKAAKAYLNWLYSPQA
                     QTIITDYYYRVNNPEVMDKLKDKFPQTELFRVEDKFGSWPEVMKTHFTSGGELDKLLA
                     AGRK"
     gene            complement(61885..62676)
                     /gene="ucpA"
                     /locus_tag="OO357_000060"
     CDS             complement(61885..62676)
                     /gene="ucpA"
                     /locus_tag="OO357_000060"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708278.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase UcpA"
                     /protein_id="HCR5885155.1"
                     /translation="MGKLTGKTALITGALQGIGEGIARTFARHGANLILLDISPEIEK
                     LADELCGRGHRCTAVVADVRDPASVAAAIKRAKEKEGRIDILVNNAGVCRLGSFLDMS
                     DEDRDFHIDINIKGVWNVTKAVLPEMIARKDGRIVMMSSVTGDMVADPGETAYALTKA
                     AIVGLTKSLAVEYAQSGIRVNAICPGYVRTPMAESIARQSNPEDPESVLTEMAKAIPL
                     CRLADPLEVGELAAFLASDESSYLTGTQNVIDGGSTLPETVSVGI"
     gene            complement(62863..63663)
                     /gene="murR"
                     /locus_tag="OO357_000061"
     CDS             complement(62863..63663)
                     /gene="murR"
                     /locus_tag="OO357_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MurR"
                     /protein_id="HCR5885156.1"
                     /translation="MLYLTKIRNAESEFTGNEQKIADFLRARVSELKSVSSRQMAKQL
                     GISQSSIVKFAQKLGAQGFTELRMALIGEYSASREKTNATALHLHSSITSDDSLEVIA
                     RKLNREKELALEQTCALFDYARLQKIIEVISKAPFIQITGLGGSALVGRDLSFKLMKI
                     GYRVACEADTHVQATVSQALKKGDVQIAISYSGSKKEIVLCAEAARKQGATVIAITSL
                     ADSPLRRLAHFTLDTVSGETEWRSSSMSTRTAQNSVTDLLFVGLVQHQ"
     gene            63827..64723
                     /gene="murQ"
                     /locus_tag="OO357_000062"
     CDS             63827..64723
                     /gene="murQ"
                     /locus_tag="OO357_000062"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HCR5885157.1"
                     /translation="MQLEKMITEGSNTASAEIDRVSTLEMCRIINDEDKTVPPAVDRV
                     LPDIAAAIDVIHAQVSGGGRLIYLGAGTSGRLGILDASECPPTYGVKPGLVVGLIAGG
                     EYAIQHAVEGAEDSREGGVNDLKNINLTAQDVVVGIAASGRTPYVIAGLEYARQLGCR
                     TVGISCNPGSAVSTTAEFAITPIVGAEVVTGSSRMKAGTAQKLVLNMLSTGLMIKSGK
                     VFGNLMVDVVATNEKLHVRQVNIVKNATGCNAEQAEAALIACERNCKTAIVMVLKNLD
                     AAEAKKRLDQHGGFIRQVLDKE"
     gene            64727..66151
                     /gene="murP"
                     /locus_tag="OO357_000063"
     CDS             64727..66151
                     /gene="murP"
                     /locus_tag="OO357_000063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylmuramic acid transporter subunit
                     IIBC"
                     /protein_id="HCR5885158.1"
                     /translation="MAKEISSELLNTILTRVGGPGNIASCGNCMTRLRLGVHDSSLVD
                     PNIKTLEGVKGVILTSDQVQVVFGPGKAHRAAKAMSELLGDAPVQDAAEIAAQNKRQL
                     KAKQTSGVQQFLAKFATIFTPLIPGFIAAGLLLGIATLIATVMHVPADAQGTLPDALN
                     FMKVFSKGLFTFLVILVGYNAAQAFGGTGVNGAIIAALFLLGYNPAATTGYYAGFHDF
                     FGLPIDPRGNIIGVLIAAWACARIEGMVRRFMPDDLDMLLTSLITLLITATLAYLIIM
                     PLGGWLFEGMSWLFMHLNSNPFGCAVLAGLFLIAVVFGVHQGFIPVYLALMDSQGFNS
                     LFPILSMAGAGQVGAALALYWRAQPHSALRSQVRGAIIPGLLGVGEPLIYGVTLPRMK
                     PFVTACLGGAAGGLFIGLIAWWGLPMGLNSAFGPSGLVALPLMTSAQGILPAMAVYAG
                     GILVAWVCGFIFTTLFGCRNVNLD"
     gene            complement(66331..67230)
                     /gene="yfeX"
                     /locus_tag="OO357_000064"
     CDS             complement(66331..67230)
                     /gene="yfeX"
                     /locus_tag="OO357_000064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708282.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphyrinogen peroxidase"
                     /protein_id="HCR5885159.1"
                     /translation="MSQVQSGILPEHCRAAIWIEANVKGEVDALRAASKTFADKLATF
                     EAKFPDAHLGAVVAFGNNTWRALSGGVGAEELKDFPGYGKGLAPTTQFDVLIHILSLR
                     HDVNFSVAQAAMEAFGDCIEVKEEIHGFRWVEERDLSGFVDGTENPAGEETRREVAVI
                     KDGVDSGGSYVFVQRWEHNLKQLNRMSVHDQEMMIGRTKEANEEIDGDERPETSHLTR
                     VDLKEDGKGLKIVRQSLPYGTASGTHGLYFCAYCARLHNIEQQLLSMFGDTDGKRDAM
                     LRFTKPVTGGYYFAPSLDKLMAL"
     gene            complement(67326..67901)
                     /locus_tag="OO357_000065"
     CDS             complement(67326..67901)
                     /locus_tag="OO357_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RpoE-regulated lipoprotein"
                     /protein_id="HCR5885160.1"
                     /translation="MKSLRLMLCAMPLMLTGCSTMSSVNWSAANPWNWFGSSTKVSEQ
                     GVGELTASTPLQEQAIADALDGDYRLRSGMKTTNGNVVRFFEVMKGDNVAMVINGDQG
                     TISRIDVLDSDIPADTGVKIGTPFSDLYSKAFGNCQKADGDDNRAVECKAEGSQHISY
                     QFSGEWRGPEGLMPSDDTLKNWKVSKIIWRR"
     gene            complement(67962..68411)
                     /locus_tag="OO357_000066"
     CDS             complement(67962..68411)
                     /locus_tag="OO357_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2919 domain-containing protein"
                     /protein_id="HCR5885161.1"
                     /translation="MKSTEFHPVHYDAHGRLRLPLLFWLVLLLQARTWVLFVIAGASR
                     EQGTALLNLFYPDHDNFWLGLIPGIPAVLAFLLSGRRASFPRTWHVLYFLLLLAQVVL
                     LCWQPWLWLNGESVSGIGLALVVADIVALIWLLTNRRLRACFNEEKE"
     gene            complement(68398..68823)
                     /locus_tag="OO357_000067"
     CDS             complement(68398..68823)
                     /locus_tag="OO357_000067"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311332.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family acetyltransferase"
                     /protein_id="HCR5885162.1"
                     /translation="MEIRVFRQEDFEEVITLWERCDLLRPWNDPEMDIERKMNHDVSL
                     FLVAEVNGEVVGTVMGGYDGHRGSAYYLGVHPEFRGRGIANALLNRLEKKLIARGCPK
                     IQINVPEDNDMVLGMYERLGYEHADVLSLGKRLIEDEEY"
     gene            69037..69906
                     /gene="amiA"
                     /locus_tag="OO357_000068"
     CDS             69037..69906
                     /gene="amiA"
                     /locus_tag="OO357_000068"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiA"
                     /protein_id="HCR5885163.1"
                     /translation="MSTFKPLKTLTSRRQVLKAGLAALTLSGMSQAIAKDEPLKTSNG
                     HSKPKAKKSGGKRVVVLDPGHGGIDTGAIGRNGSKEKHVVLAIAKNVRSILRNHGIDA
                     RLTRSGDTFIPLYDRVEIAHKHGADLFMSIHADGFTNPKAAGASVFALSNRGASSAMA
                     KYLSERENRADEVAGKKATDKDHLLQQVLFDLVQTDTIKNSLTLGSHILKKIKPVHKL
                     HSRNTEQAAFVVLKSPSVPSVLVETSFITNPEEERLLGTAAFRQKIATAIAEGVISYF
                     HWFDNQKAHSKKR"
     gene            69910..70809
                     /gene="hemF"
                     /locus_tag="OO357_000069"
     CDS             69910..70809
                     /gene="hemF"
                     /locus_tag="OO357_000069"
                     /EC_number="1.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-dependent coproporphyrinogen oxidase"
                     /protein_id="HCR5885164.1"
                     /translation="MKPDAHQVKQFLLNLQDTICQQLTAVDGAEFVEDSWQREAGGGG
                     RSRVLRNGGVFEQAGVNFSHVHGEAMPASATAHRPELAGRSFEAMGVSLVVHPHNPYV
                     PTSHANVRFFIAEKPGAEPVWWFGGGFDLTPFYGFEEDAIHWHRTARDLCQPFGEDVY
                     PRYKKWCDDYFYLKHRNEQRGIGGLFFDDLNTPDFDHCFAFMQAVGKGYTDAYLPIVE
                     RRKAMAYGERERNFQLYRRGRYVEFNLVWDRGTLFGLQTGGRTESILMSMPPLVRWEY
                     DYQPKDGSPEAALSEFIKVRDWV"
     gene            complement(70815..71867)
                     /gene="eutR"
                     /locus_tag="OO357_000070"
                     /pseudo
     CDS             complement(70815..71867)
                     /gene="eutR"
                     /locus_tag="OO357_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311335.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator EutR"
     gene            complement(71913..72413)
                     /gene="eutK"
                     /locus_tag="OO357_000071"
     CDS             complement(71913..72413)
                     /gene="eutK"
                     /locus_tag="OO357_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutK"
                     /protein_id="HCR5885165.1"
                     /translation="MINALGLLEVDGMVAAIDAADAMLKAANVRLLSHEVLDPGRLTL
                     VVEGDLAACRAALDAGCAAAMRTGRVISRKEIGRPDDDTQWLVTGFNRQPKQPVKEPD
                     APVIVAESADELLALLTSVCQGMTAGEVAAHFGWPLEKARNALEQLFSAGTLRKRSSR
                     YRLKPH"
     gene            complement(72426..73085)
                     /gene="eutL"
                     /locus_tag="OO357_000072"
     CDS             complement(72426..73085)
                     /gene="eutL"
                     /locus_tag="OO357_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HCR5885166.1"
                     /translation="MPALDLIRPSVTAMRVIASVNAEFARELKLPPHIRSLGLISADS
                     DDVTYIAADEATKQAMVEVVYGRSLYAGAAHGPSPTAGEVLIMLGGPNPAEVRAGLDA
                     MVAHIENGAAFQWANDAENTAFLAHVVSRTGSYLSSTAGITLGDPMAYLVAPPLEATY
                     GIDAALKSADVQLVTYVPPPSETNYSAAFLTGSQAACKAACNAFTDAVLEIARNPIQR
                     A"
     gene            74267..75058
                     /locus_tag="OO357_000073"
     CDS             74267..75058
                     /locus_tag="OO357_000073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885167.1"
                     /translation="MRALKIDAISLNFSHSPEWWRSGLIATLPRLDIERIIDTYIKRK
                     RDKFIVKIRGLHIMRISEQQRQILDYLAKNESITARQAADLVYGGNVTRVQVDAARRS
                     LMTMVSNGLIRKQGRAFVAERHINHAYSQDVEELRRQILWVFSKQGQEYFLSEHPNFA
                     AKELTVSGLSCALDYLVGEGCVTYKQLWHALNTLVEDGLLIATRENIEYWIMPRSGRR
                     NHARYISGNRYRTPDFNPTQEDRDRAEMMCREQEKRIMDKLFSMQ"
     gene            75778..76173
                     /locus_tag="OO357_000074"
     CDS             75778..76173
                     /locus_tag="OO357_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5885168.1"
                     /translation="MTNIERLERCADIMRRRWIYDPEAGLLFSRETKDVIKGSMTNKG
                     HLVVTVHEGKFKVTLTYQKACYVYTYGAYDETLYEVHHVNLNKQDNRIKNIRLMPKEK
                     HRRIHKNVRKLIRLGHTQWLQPQLIEQSL"
     gene            76191..76454
                     /locus_tag="OO357_000075"
     CDS             76191..76454
                     /locus_tag="OO357_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001533176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885169.1"
                     /translation="MHSLDSYFQRTTAPKSAAQERREEFHEKVMRSADYIADKFVETV
                     RPLVDEVADKLQSEMPEDMEGTAKRRLICELSRRFGVSISAFK"
     gene            76477..77019
                     /locus_tag="OO357_000076"
     CDS             76477..77019
                     /locus_tag="OO357_000076"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885170.1"
                     /translation="MDAFEVLANKGAELVAQRDKTANEGERALLNKQIKAIRMAQFKL
                     ISNEVIETIKPQIPQIVADAIKAAGLEKRIAGLKTNAEKMEMYKQSGAPNPDEYFSPD
                     VEFMAQVEERLGSCLTEEQRRYFDGVDSSAGIDLNSYFGREIEHFDAAPSMPEPEPEM
                     ATDPEAEQRKRVFNAIGIRY"
     gene            77158..77445
                     /locus_tag="OO357_000077"
     CDS             77158..77445
                     /locus_tag="OO357_000077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001373377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885171.1"
                     /translation="MLALRIMQGIAKTLAEHVLDLKHSPLSKQAMKRQTLRLWAEYSL
                     GTINKIIDMKSGPSNQSAEEMEFIRRLILIRRDIHSQLHSVGIDINDGTGD"
     gene            77484..77690
                     /locus_tag="OO357_000078"
     CDS             77484..77690
                     /locus_tag="OO357_000078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885172.1"
                     /translation="MKQDIADRLEILEGQRAEAKQLRKQARRAHRNNEAELLTKYISF
                     TNYCIYECYKEDAEDWLDSLPEQY"
     gene            78002..78487
                     /locus_tag="OO357_000079"
     CDS             78002..78487
                     /locus_tag="OO357_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5885173.1"
                     /translation="MKNKSGNSCYREAVDIHSSLELSFILHKYHLRVDEKKSRNVFSA
                     IRDELILDGDSRQNLATFCQTWLDDEIRELLNLAIDKNMIYKDEYPQMAEPEGRCVRM
                     LSDSWSFPDSRHTLGCSTIGSSEAAMLGRLALKWQWCKKREVQGKSTEPDLRSCANML
                     A"
     gene            78468..78746
                     /locus_tag="OO357_000080"
     CDS             78468..78746
                     /locus_tag="OO357_000080"
                     /inference="COORDINATES: protein motif:HMM:NF012503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885174.1"
                     /translation="MQICWHKFCRYCDVKLCEIPLDDHHLIMNAKEVLKHVDENTIGV
                     VTTLGITYFCKADCVSVFKISVKVNISFYIYIVIICFYFKNVREYKNV"
     gene            78739..80199
                     /locus_tag="OO357_000081"
     CDS             78739..80199
                     /locus_tag="OO357_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5885175.1"
                     /translation="MSNANIAPKSKKMTVGTLAIMNITAVVSLRFIPSEAEYGLGAIF
                     YYTFAAIMFLVPVAFVAAELATTYPQKGGAFRWIGEAFGARWGFVGMLMTWLQVIPYF
                     PTVLTFGAVSVAFIDVDMGVAESIASNKWYILFFVLFVYWGAICVALRGVGIFSQVSK
                     WCGIIGTIIPAIILVILGFSYLWFSGKPAVIPLGWGDLFPNIANFQNIVLAASIFLAY
                     AGMEMNAVHVNDLDNPTKKYPIAITIASIGTIAIFLLSTLGVAFIIPTDKISLTQSLL
                     VAYDMLFEWAGVPWLGSVMAFMLAIGVLGGVVTWIAGPNTGVLAIAKAGYLPKFFQKT
                     NRHGMGHHLMFVQGIIVSVLSVTFVIMPSVQAAFQILSQLTVMLYLVMYMLMFASAIY
                     LRHSQPDTKRPYRAPALFFWATLGFLGSFLAFVLSFIPPEQIPTGSPTSYVLYLLVLV
                     ALFLLIPILIYAFRKSDWKDPNSDFEPFSWEKRTSN"
     gene            80233..81042
                     /gene="murI"
                     /locus_tag="OO357_000082"
     CDS             80233..81042
                     /gene="murI"
                     /locus_tag="OO357_000082"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001507729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5885176.1"
                     /translation="MFSAESPIGVIDSGVGGLTSVKEIINLLPGEDIIYCGDNMNAPY
                     GNRSADDIISLTKKMLTFLQSRNVKLVAVACNTISSTLESEEYSGYAKSFPFPILSII
                     EPAVEDVIRQQYKNVGIIATEFTIKTGCHKELIKKLNSTINVFGEPSKNLAMLIEEGN
                     LNAPAILNDIKKHVNHLISLHPVNEIILGCTHYPIVQNFFEAVAPDIKFINPAHDQAI
                     SIKNHLGQLNLLNSSNIGTLQINTSGSMEIYKTVLGELSITKPHTFSIRQF"
     gene            complement(81161..81855)
                     /locus_tag="OO357_000083"
     CDS             complement(join(81161..81609,81609..81855))
                     /locus_tag="OO357_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5885177.1"
                     /translation="MTSVNIHCPRCQSAQVYRHGQNPKGRDRLRCRDCHRVFQFTYTY
                     QARKPGMKELITEMAFNGAGVRDTARTLKIGSNTVIRTFKKLAPKRITSSPVAHADVA
                     FICELDEQWSYVGSKARQHWLGYAYNTKTGGVLAYTFGPRTDQTCRELLALLTPFNIG
                     MLTSDDWGSYGREVPKNKHLTGKIFTQRIERNNLTLRTRIKRLGRKTICFSRSVEIHE
                     KVIGAFIEKHMFY"
     gene            complement(82374..83747)
                     /gene="eutA"
                     /locus_tag="OO357_000084"
     CDS             complement(82374..83747)
                     /gene="eutA"
                     /locus_tag="OO357_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HCR5885178.1"
                     /translation="MKKLITANDIREAHARGEQAMSVVLRASIITPEAREVADLLGFT
                     ITECDESIPVTASVPASVPADKTESQRIRETIIAQLPEGQFTESLVAQLMEKVMKEKQ
                     SLGDFVVASAGPHLESVIAGHGAGAQTLSEQRLCRVLNIDIGGGTANYALFDAGKISG
                     TACLNVGGRLLETDSQGRVVYAHKPGQMIVDECFGAGTDARSLTGAQLVQVTRRMAAL
                     IVEVIDGTLSPLAQALMQTGLLPAGVTPEIITLSGGVGECYRHQPANPFCFADIGPLL
                     ATALHDHPRLREMNVQFPAQTVRSTVIGAGAHTLSLSGSTIWLEGVQLPLRNLPVAIP
                     IDETDLVSAWQQALLQLDLDPKTDVYVLALPASLPVRYAAVLTVINALVDFVARFPNP
                     HPLLVVAGQDFGKALGMLLRPQLQQLPLAVIDEVIVRAGDYIDIGTPLFGGSVVPVTV
                     KSLAFPS"
     gene            complement(83722..84201)
                     /gene="eutP"
                     /locus_tag="OO357_000085"
     CDS             complement(83722..84201)
                     /gene="eutP"
                     /locus_tag="OO357_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization acetate kinase EutP"
                     /protein_id="HCR5885179.1"
                     /translation="MKRIAFVGSVGAGKTTLFNALQGNYTLARKTLAVEFNDKGDIDT
                     PGEYFSHPRWYHALITTLQDVDMLIYVHGANDPESRLPAGLLDIGVSKRQIAVISKTD
                     MPDADVAATRKLLLETGFEEPIFELNSHDPQSVQQLVDYLASLTKQEEAGEKTHHSE"
     gene            complement(84214..84549)
                     /gene="eutS"
                     /locus_tag="OO357_000086"
     CDS             complement(84214..84549)
                     /gene="eutS"
                     /locus_tag="OO357_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutS"
                     /protein_id="HCR5885180.1"
                     /translation="MDKERIIQEFVPGKQVTLAHLIAHPGEELAKKIGVPDAGAIGIM
                     TLTPGETAMIAGDLALKAADVHIGFLDRFSGALVIYGSVGAVEEALSQTVSGLGRLLN
                     YTLCEMTKS"
     gene            complement(84842..87121)
                     /gene="maeB"
                     /locus_tag="OO357_000087"
     CDS             complement(84842..87121)
                     /gene="maeB"
                     /locus_tag="OO357_000087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxaloacetate-decarboxylating
                     malate dehydrogenase"
                     /protein_id="HCR5885181.1"
                     /translation="MDDQLKQSALDFHEFPVPGKIQVSPTKPLATQRDLALAYSPGVA
                     APCLEIEKDPLKAYKYTARGNLVAVISNGTAVLGLGNIGALAGKPVMEGKGVLFKKFA
                     GIDVFDIEVDELDPDKFIEVVAALEPTFGGINLEDIKAPECFYIEQKLRERMNIPVFH
                     DDQHGTAIISTAAILNGLRVVEKNISDVRMVVSGAGAAAIACMNLLVALGLQKHNIVV
                     CDSKGVIYQGREPNMAETKAAYAVVDDGKRTLDDVIEGADIFLGCSGPKVLTQEMVKK
                     MARAPMILALANPEPEILPPLAKEVRPDAIICTGRSDYPNQVNNVLCFPFIFRGALDV
                     GATAINEEMKLAAVRAIAELAHAEQSEVVASAYGVQDLSFGPEYIIPKPFDPRLIVKI
                     APAVAKAAMESGVATRPIADFDVYIDKLTEFVYKTNLFMKPIFSQARKAPKRVVLPEG
                     EEARVLHATQELVTLGLAKPILIGRPNVIEMRIQKLGLQIKAGVDFEIVNNESDPRFK
                     EYWTEYFQIMKRRGVTQEQAQRALISNPTVIGAIMVQRGEADAMICGTVGDYHEHFSV
                     VKNVFGYRDGVHTAGAMNALLLPSGNTFIADTYVNDEPDAEELAEITLMAAETVRRFG
                     IEPRVALLSHSNFGSSDCPSSSKMRQALELVRDRAPDLMIDGEMHGDAALVEAIRNDR
                     MPDSPLKGSANILVMPNMEAARISYNLLRVSSSEGVTVGPVLMGVAKPVHVLTPIASV
                     RRIVNMVALAVVEAQTQPL"
     gene            87410..88360
                     /gene="tal"
                     /locus_tag="OO357_000088"
     CDS             87410..88360
                     /gene="tal"
                     /locus_tag="OO357_000088"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5885182.1"
                     /translation="MNELDGIKQFTTVVADSCDIESIRHYHPQDATTNPSLLLKAAGL
                     SQYEHLIDDAIAWGKKNGKTQEQQVVAACDKLAVNFGAEILKIVPGCVSTEVDARLSF
                     DKEKSIEKARHLVDLYQQQGVEKSRILIKLASTWEGIRAAEELEKEGINCNLTLLFSF
                     TQARACAEAGVFLISPFVGRIYDWYQARKPMDPYVVEEDPGVKSVRNIYDYYKQHHYE
                     TIVMGASFRRTEQILALTGCDRLTIAPNLLKELQEKVSPVVRKLIPPSQTFPRPAPMS
                     EAEFRWEHNQDAMAVEKLSEGIRLFAVDQRKLEDLLAAKL"
     gene            88380..90383
                     /gene="tkt"
                     /locus_tag="OO357_000089"
     CDS             88380..90383
                     /gene="tkt"
                     /locus_tag="OO357_000089"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5885183.1"
                     /translation="MSRKDLANAIRALSMDAVQKANSGHPGAPMGMADIAEVLWNDFL
                     KHNPTDPTWYDRDRFILSNGHASMLLYSLLHLTGYDLPLEELKNFRQLHSKTPGHPEI
                     GYTPGVETTTGPLGQGLANAVGLAIAERTLAAQFNQPDHEIVDHFTYVFMGDGCLMEG
                     ISHEVCSLAGTLGLGKLIGFYDHNGISIDGETEGWFTDDTAKRFEAYHWHVIHEIDGH
                     DPQAVKEAILEAQSVKDKPSLIICRTVIGFGSPNKAGKEEAHGAPLGEEEVALARQKL
                     GWHHPPFEIPKEIYHAWDAREKGEKAQQSWNEKFAAYKKAHPQLAEEFPRRMSGGLPK
                     DWEKTTQKYINELQANPAKIATRKASQNTLNAYGPMLPELLGGSADLAPSNLTIWKGS
                     VSLKEDPAGNYIHYGVREFGMTAIANGIAHHGGFVPYTATFLMFVEYARNAARMAALM
                     KARQIMVYTHDSIGLGEDGPTHQAVEQLASLRLTPNFSTWRPCDQMEAAVGWKLAVER
                     HNGPTALILSRQNLAQVERTPDQVKEIARGGYVLKDSGGKPDIILIATGSEMEITLQA
                     AEKLAGEGRNVRVVSLPSTDIFDAQDEEYRESVLPSNVAARVAVEAGIADYWYKYVGL
                     KGAIVGMTGYGESAPADKLFPFFGFTAENIVAKAHKVLGVKGA"
     gene            complement(90486..91529)
                     /locus_tag="OO357_000090"
     CDS             complement(90486..91529)
                     /locus_tag="OO357_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708305.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1176 domain-containing protein"
                     /protein_id="HCR5885184.1"
                     /translation="MRYRIFLLFFFALLPTSLVWAAPAQRAFSDWQVTCNNQNFCVAR
                     NTGDHNGLVMTLSRSAGAHTDAVLRIERGGLKSPDASEGEIAPRLLLDGEPLALSGDK
                     WRISPWLLVTDDTATITAFLQIIQEGKAITLRDGNQTISLSGLKAALLFIDAQQKRVG
                     SETAWIKKGDEPPLSVPPAPALKEVAVVNPTPTPLSLEERNDLLDYGNWRMNGLRCSL
                     DPLRREVNVTALTDDKALMMISCEAGAYNTIDLAWIVSRKKPLASRPVRLRLPFNNGQ
                     ETNELELMNATFDEKSRELVTLAKGRGLSDCGIQARWRFDGQRFRLVRYAAEPTCDNW
                     HGPDARPTLWITR"
     gene            complement(91655..92230)
                     /gene="nudK"
                     /locus_tag="OO357_000091"
     CDS             complement(91655..92230)
                     /gene="nudK"
                     /locus_tag="OO357_000091"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose pyrophosphatase NudK"
                     /protein_id="HCR5885185.1"
                     /translation="MTQQITLIKDKILSDNYFTLHNITYDLTRKDGEVIRHKREVYDR
                     GNGATILLYNAKKKSVVLIRQFRVATWVNGNESGQLIETCAGLLDNDEPEVCIRKEAI
                     EETGYEVGEVRKLFELYMSPGGVTELIHFFIAEYSDNQRANAGGGVEDEDIEVLELPF
                     SQALEMIKTGEIRDGKTVLLLNYLQMSHLMD"
     gene            complement(92298..94277)
                     /gene="aegA"
                     /locus_tag="OO357_000092"
     CDS             complement(92298..94277)
                     /gene="aegA"
                     /locus_tag="OO357_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent uric acid utilization protein
                     AegA"
                     /protein_id="HCR5885186.1"
                     /translation="MNRFIMANSQQCLGCHACEIACVMAHNDEQHVLSQHHFHPRITV
                     IKHQQQRSAVTCHHCEDAPCARSCPNGAISHVDDSIQVNQQKCIGCKSCVVACPFGTM
                     QIVLTPVAAGKVKATAHKCDLCAGRENGPACVENCPADALQLVTDVALSGMAKSRRLR
                     TARQEHQPWHASTAAQEMPVMSKVEQMQATPARGEPDKLAIEARKTGFDEIYLPFRAD
                     QAQREASRCLKFGEHSVCEWTCPLHNHIPQWIELVKAGNIDAAVELSHQTNTLPEITG
                     RVCPQDRLCEGACTIRDEHGAVTIGNIERYISDQALAKGWRPDLSHVTKVDKRVAIIG
                     AGPAGLACADVLTRNGVAVTVYDRHPEIGGLLTFGIPSFKLDKSLLARRREIFSAMGI
                     HFELNCEVGKDVSLDLLLEQYDAVFVGVGTYRSMKAGLPNEDAPGVYDALPFLIANTK
                     QVMGLEELPEEPFINTAGLNVVVLGGGDTAMDCVRTALRHGASNVTCAYRRDEANMPG
                     SKKEVKNAREEGANFEFNVQPVALELNEQGHVCGIRFLRTRLGEPDAQGRRRPVPIEG
                     SEFVMPADAVIMAFGFNPHGMPWLESHGVTVDKWGRIIADVESQYRYQTTNPKIFAGG
                     DAVRGADLVVTAMAEGRHAAQGIIDWLGVKSVKSH"
     gene            94483..96200
                     /gene="narQ"
                     /locus_tag="OO357_000093"
                     /pseudo
     CDS             94483..96200
                     /gene="narQ"
                     /locus_tag="OO357_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005011308.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarQ"
     gene            96402..99515
                     /gene="acrD"
                     /locus_tag="OO357_000094"
     CDS             96402..99515
                     /gene="acrD"
                     /locus_tag="OO357_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease AcrD"
                     /protein_id="HCR5885187.1"
                     /translation="MANFFIDRPIFAWVLTILLCLTGTLAIFSLPVEQYPDLAPPNVR
                     VTANYPGASAQTLENTVTQVIEQNMTGLDNLMYMSSQSSGTGQASVTLSFKAGTDPDE
                     AVQQVQNQLQSAMRKLPQAVQNQGVTVRKTGDTNILTIAFVSTDGSMDKQDIADYVAS
                     NIQDPLSRVNGVGDIDAYGSQYSMRIWLDPAKLNSFQMTAKDVTDAIESQNAQIAVGQ
                     LGGTPSVDKQALNATINAQSLLQTPEQFRDITLRVNQDGSEVRLGDVATVEMGAEKYD
                     YLSRFNGKPASGLGVKLASSANEMATAELVLNRLDELAQYFPHGLEYKVAYETTSFVK
                     ASIEDVVKTLLEAIALVFLVMYLFLQNFRATLIPTIAVPVVLMGTFSVLYAFGYSVNT
                     LTMFAMVLAIGLLVDDAIVVVENVERIMSEEGLTPREATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGTTGAIYRQFSITIVAAMVLSVLVAMLLTPALCATLLKPLKKGEHHGQ
                     KGFFAWFNQMFNRNAERYEKGVAKILHRSLRWIVIYVLLLGGMVFLFLRLPTSFLPLE
                     DRGMFTTSVQLPSGSTQQQTLKVVEQIEKYYFTHEKDNIMSVFATVGSGPGGNGQNVA
                     RMFIRLKDWSERDSKTGTSFAIIERATKAFNQIKEARVIASSPPAISGLGSSAGFDME
                     LQDHAGAGHDALMAARNQLLALAAENPELTRVRHNGLDDSPQLQIDIDQRKAQALGVD
                     IDDINDTLQTAWGSSYVNDFMDRGRVKKVYVQAAAPYRMLPDDINLWYVRNKDGGMVP
                     FSAFATSRWETGSPRLERYNGYSAVEIVGEAAPGVSTGTAMDIMESLVKQLPNGFGLE
                     WTAMSYQERLSGAQAPALYAISLLVVFLCLAALYESWSVPFSVMLVVPLGVIGALLAT
                     WMRGLENDVYFQVGLLTVIGLSAKNAILIVEFANEMNQKGHDLFEATLHACRQRLRPI
                     LMTSLAFIFGVLPMATSTGAGSGGQHAVGTGVMGGMISATILAIYFVPLFFVLVRRRF
                     PLKPRPE"
     gene            complement(99614..99673)
                     /gene="ypfM"
                     /locus_tag="OO357_000095"
     CDS             complement(99614..99673)
                     /gene="ypfM"
                     /locus_tag="OO357_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YpfM"
                     /protein_id="HCR5885188.1"
                     /translation="MIERELGNWKDFIEVMLRK"
     gene            100054..100410
                     /locus_tag="OO357_000096"
     CDS             100054..100410
                     /locus_tag="OO357_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsC family reductase"
                     /protein_id="HCR5885189.1"
                     /translation="MVTLYGIKNCDTIKKARRWLEANNIDYRFHDYRVDGLDSELLNG
                     FINELGWEALLNTRGTTWRKLDETTRNKITDAASAAALMTEMPAIIKRPLLCAPGKPM
                     LLGFSDSSYQQFFHEV"
     gene            100414..101541
                     /gene="dapE"
                     /locus_tag="OO357_000097"
     CDS             100414..101541
                     /gene="dapE"
                     /locus_tag="OO357_000097"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020078199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HCR5885190.1"
                     /translation="MSCPVIELTQQLIRRPSLSPDDAGCQALLIERLQAIGFTVERMD
                     FADTQNFWAWRGQGETLAFAGHTDVVPPGDADRWINPPFEPTIRDGMLFGRGAADMKG
                     SLAAMVVAAERFVAQHPNHTGRLAFLITSDEEASAHNGTVKVVEALMARNERLDYCLV
                     GEPSSIEVVGDVVKNGRRGSLTCNLTIHGVQGHVAYPHLADNPVHRAAPFLNELVAIE
                     WDQGNEFFPATSMQIANIQAGTGSNNVIPGELFVQFNFRFSTELTDEMIKAQVLALLE
                     KHQLRYTVDWWLSGQPFLTARGKLVDAVVNAVEHYNEIKPQLLTTGGTSDGRFIARMG
                     AQVVELGPVNATIHKINECVNAADLQLLARMYQRIMEQLVA"
     gene            101569..101769
                     /locus_tag="OO357_000098"
     CDS             101569..101769
                     /locus_tag="OO357_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpfN family protein"
                     /protein_id="HCR5885191.1"
                     /translation="MDWLAKYWWILVIVFLVGVLLNVIKDLKRVDHKKFLANKPELPP
                     HRDFNDKWDDDDDWPKKDQPKK"
     gene            complement(101879..102577)
                     /gene="ypfH"
                     /locus_tag="OO357_000099"
     CDS             complement(101879..102577)
                     /gene="ypfH"
                     /locus_tag="OO357_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708312.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5885192.1"
                     /translation="MKHDHFVVQSPDKPAQQLLLLFHGVGDNPVAMGEIGSWFAPLFP
                     DALVVSVGGAEPSGNPAGRQWFSVQGITEDNRQARVDAIMPTFIETVRYWQKQSGVGA
                     NATALIGFSQGAIMALESIKAEPGLASRVIAFNGRYASLPETASTATTIHLIHGGEDP
                     VIDLAHAVAAQEALISAGGDVTLDIVEDLGHAIDNRSMQFALDHLRYTIPKHYFDQAL
                     SGGKPGDDDVIEMM"
     gene            complement(102651..104666)
                     /gene="tmcA"
                     /locus_tag="OO357_000100"
     CDS             complement(102651..104666)
                     /gene="tmcA"
                     /locus_tag="OO357_000100"
                     /EC_number="2.3.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cytosine(34) acetyltransferase TmcA"
                     /protein_id="HCR5885193.1"
                     /translation="MAELTALYTLTAQMKREGICRLLVLSGEEGWCFDHALKLRDALP
                     GDWLWISPQLDAENHCSPSALQTLLGREFRHAVFDARHGFDAAAFAALSGTLKAGSWL
                     VLLLPVWEEWENQPDADSLRWSDCPDPIATPHFVQHLKRVLTADNDAILWRQNQPFSL
                     AHFTPRTDWHPATGAPQPEQQQLLQQLLTMPPGVAAVTAARGRGKSALAGQLISRIAG
                     SAIVTAPAKAATDVLAQFAGEKFRFIAPDALLASDEQADWLVVDEAAAIPAPLLHQLV
                     SRFPRTLLTTTVQGYEGTGRGFLLKFCARFPHLHRFELQQPIRWAQGCPLEKMVSEAL
                     VFNDENFTHTPQGNIVISAFEQTLWRSEPETPLKVYQLLSGAHYRTSPLDLRRMMDAP
                     GQHFLQAAGENEIAGALWLVDEGGLSQELSQAVWAGFRRPRGNLVAQSLAAHGSNPLA
                     ATLRGRRVSRIAVHPARQREGTGRQLIAGALQYTHDLDYLSVSFGYTGELWRFWQRCG
                     FVLVRMGNHREASSGCYTAMALLPMSDAGKQLAEREHYRLRRDAQALAQWNGEMLPVD
                     PLNDAILSDDDWLELAGFAFAHRPLLTSLGCLLRLLQTSELALPALRGRLQKNASDAQ
                     LCTTLKLSGRKMLLVRQREEAAQALFALNEVRTERLRDRITQWQFFH"
     gene            complement(104681..105544)
                     /locus_tag="OO357_000101"
     CDS             complement(104681..105544)
                     /locus_tag="OO357_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="neutral zinc metallopeptidase"
                     /protein_id="HCR5885194.1"
                     /translation="MRWQGRRESDNVEDRRNSSGGPSMGGPGFRLPSGKGGLILLIVV
                     LVAGYYGVDLTGLMTGQPVSQQQSTRSISPNEDEAAKFTSVIQATTEDTWGQQFEKMG
                     KTYQQPKLVMYRGMTRTGCGAGQSIMGPFYCPADGTVYIDLSFYDDMKDKLGADGDFA
                     QGYVIAHEVGHHVQKLLGIEPKVRQLQQNATQAEVNRLSVRMELQADCFAGVWGHSMQ
                     QQGVLETGDLEEALNAAQAIGDDRLQQQSQGRVVPDSFTHGTSQQRYSWFKRGFDSGD
                     PAQCNTFGKSI"
     gene            complement(105712..106425)
                     /gene="purC"
                     /locus_tag="OO357_000102"
     CDS             complement(105712..106425)
                     /gene="purC"
                     /locus_tag="OO357_000102"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HCR5885195.1"
                     /translation="MQKQAELYRGKAKTVYSTENPDLLVLEFRNDTSAGDGARIEQFD
                     RKGMVNNKFNYFIMSKLAEAGIPTQMERLLSDTECLVKKLDMVPVECVVRNRAAGSLV
                     KRLGIEEGIELNPPLFDLFLKNDAMHDPMVNESYCETFGWVSKENLARMKELTYKAND
                     VLKKLFDDAGLILVDFKLEFGLYKGEVVLGDEFSPDGSRLWDKETLEKMDKDRFRQSL
                     GGLIEAYEAVARRLGVQLD"
     gene            complement(106638..107672)
                     /gene="bamC"
                     /locus_tag="OO357_000103"
     CDS             complement(106638..107672)
                     /gene="bamC"
                     /locus_tag="OO357_000103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamC"
                     /protein_id="HCR5885196.1"
                     /translation="MAYSVQKSRLAKVAGVSLVLLLAACSSDSRYKRQVSGDEAYLEA
                     APLAELHAPAGMILPVTSGDYAIPVTNGSGAVGKALDIRPPAQPLALVSGARTQFTGD
                     TASLLVENGRGNTLWPQVVSVLQAKNYTITQRDDAGQTLTTDWVQWNRLDEDEQYRGR
                     YQISVKPQGYQQAVTVKLLNLEQAGKPVADAASMQRYSTEMMNVISAGLDKSATDAAN
                     AAQNRASTTMDVQSAADDTGLPMLVVRGPFNVVWQRLPAALEKVGMKVTDSTRSQGNM
                     AVTYKPLSDSDWQELGASDPGLASGDYKLQVGDLDNRSSLQFIDPKGHTLTQSQNDAL
                     VAVFQAAFSK"
     gene            complement(107689..108567)
                     /gene="dapA"
                     /locus_tag="OO357_000104"
     CDS             complement(107689..108567)
                     /gene="dapA"
                     /locus_tag="OO357_000104"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001311023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HCR5885197.1"
                     /translation="MFTGSIVAIVTPMDEKGNVCRASLKKLIDYHVASGTSAIVSVGT
                     TGESATLNHDEHADVVMMTLDLADGRIPVIAGTGANATAEAISLTQRFNDSGIVGCLT
                     VTPYYNRPSQEGLYQHFKAIAEHTDLPQILYNVPSRTGCDLLPETVGRLAKVKNIIGI
                     KEATGNLTRVNQIKELVSDDFVLLSGDDASALDFMQLGGHGVISVTANVAARDMAQMC
                     KLAAEGHFAEARVINQRLMPLHNKLFVEPNPIPVKWACKELGLVATDTLRLPMTPITD
                     SGRETVRAALKHAGLL"
     gene            108713..109285
                     /locus_tag="OO357_000105"
     CDS             108713..109285
                     /locus_tag="OO357_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     repressor"
                     /protein_id="HCR5885198.1"
                     /translation="MTLSSQHYLVITALGADRPGIVNTITRHVSSCGCNIEDSRLAML
                     GEEFTFIMLLSGSWNAITLIESTLPLKGAELDLLIVMKRTTARPRPPMPASVWVQVDV
                     ADSPHLIERFTALFDAHHMNIAELVSRTQPAENERAAQLHIQITAHSPASADAANIEQ
                     AFKALCTELNAQGSINVVNYSQHDEQDGVK"
     gene            109285..109755
                     /gene="bcp"
                     /locus_tag="OO357_000106"
     CDS             109285..109755
                     /gene="bcp"
                     /locus_tag="OO357_000106"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HCR5885199.1"
                     /translation="MNPLKAGDIAPKFSLPDQDGEQVNLTDFQGQRVLVYFYPKAMTP
                     GCTVQACGLRDNMDELKKAGVDVLGISTDKPEKLSRFAEKELLNFTLLSDEDHQVCEQ
                     FGVWGEKSFMGKTYDGIHRISFLIDADGKIEHVFDDFKTSNHHDVVLNWLKEHA"
     gene            110008..110625
                     /gene="hyfA"
                     /locus_tag="OO357_000107"
     CDS             110008..110625
                     /gene="hyfA"
                     /locus_tag="OO357_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfA"
                     /protein_id="HCR5885200.1"
                     /translation="MNRFVVAEPLWCTGCNTCLAACSDVHKTQGLQQHPRLALAKTST
                     ITAPVVCHHCEEAPCLQVCPVNAISQRDDAIQLNESLCIGCKLCAVVCPFGAISASGS
                     RPVNAHAQYVFQAEGSLKDGEENVPPQHALLRWEPGVQTVAVKCDLCDFLPEGPACVR
                     ACPNQALRLITDDSLQRQMKEKQRLAASWFANGGESPLSLTQEQH"
     gene            110625..112642
                     /gene="hyfB"
                     /locus_tag="OO357_000108"
                     /pseudo
     CDS             110625..112642
                     /gene="hyfB"
                     /locus_tag="OO357_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311371.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit B"
     gene            112653..113600
                     /gene="hyfC"
                     /locus_tag="OO357_000109"
     CDS             112653..113600
                     /gene="hyfC"
                     /locus_tag="OO357_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfC"
                     /protein_id="HCR5885201.1"
                     /translation="MRQTLCDGYLVIFALAQAVILLMLTPLFTGISRQIRARMHSRRG
                     PGIWQDYRDIHKLFKRQEVAPTSSGLMFRLMPWVLISSMLVLAMALPLFITVSPFAGG
                     GDLITLIYLLALFRFFFALSGLDTGSPFAGVGASRELTLGILVEPMLILSLLVLALIA
                     GSTHIEMISNTLAMGWNSPLTTVLALLACGFACFIEMGKIPFDVAEAEQELQEGPLTE
                     YSGAGLALAKWGLGLKQVVMAALFVALFLPFGRAQELSLACLLTSLVVTLLKVLLIFV
                     LASIAENTLARGRFLLIHHVTWLGFSLAALAWVFWLTGL"
     gene            113617..115056
                     /gene="hyfD"
                     /locus_tag="OO357_000110"
     CDS             113617..115056
                     /gene="hyfD"
                     /locus_tag="OO357_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit HyfD"
                     /protein_id="HCR5885202.1"
                     /translation="MENLALTTLMLPFIGALVVSFSPQRRAAEWGVLFAALTTLCMLS
                     LISAFYQADKVAVTLTLVNVGDVALFGLVIDRVSTLILFVVVFLGLLVTIYSTGYLTD
                     KNREHPHNGTNRYYAFLLVFIGAMAGLVLSSTLLGQLLFFEITGGCSWALISYYQSDK
                     AQRSALKALLITHIGSLGLYLAAATLFLQTGTFVLSAMSELHGDARYLVYGGILFAAW
                     GKSAQLPMQAWLPDAMEAPTPISAYLHAASMVKVGVYIFARAIIDGGNIPHVIGGVGM
                     VMTLVTILYGFLMYLPQQDMKRLLAWSTITQLGWMFFGLSLSIFGSRLALEGSIAYIV
                     NHAFAKSLFFLVAGALSYSCGTRLLPRLRGVLHTLPLPGVGFCVAALAITGVPPFNGF
                     FSKFPLFAAGFALSVEYWILLPAMILLMIESVASFAWFIRWFGRVVPGKPSEAVADAA
                     PLPGSMRLVLIVLIVMSLISSVIAATWLQ"
     gene            115068..115718
                     /gene="hyfE"
                     /locus_tag="OO357_000111"
     CDS             115068..115718
                     /gene="hyfE"
                     /locus_tag="OO357_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 membrane subunit"
                     /protein_id="HCR5885203.1"
                     /translation="MTGSMIVNNLAGLMMLTSLFVISVKSYRLSCGFYACQSLVLVSI
                     FATLSCLFATEQLLIWSASAFITKVLLVPLIMTYAARNIPQNIPEKALFGPAMMALLA
                     ALIVLLCAFVVQPVKLPMATGLKPALAVALGHFLLGLLCIVSQRNILRQIFGYCLMEN
                     GSHLVLALLAWRAPELVEIGIATDAIFAVIVMVLLARKIWRTHGTLDVNNLTALKG"
     gene            115723..117309
                     /gene="hyfF"
                     /locus_tag="OO357_000112"
     CDS             115723..117309
                     /gene="hyfF"
                     /locus_tag="OO357_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit F"
                     /protein_id="HCR5885204.1"
                     /translation="MSYSVMFALLLLTPLLFSLLCFACRKRGLSATCTVTVLHSLGIT
                     LLLILALWVVQTAADAGEIFAAGLWLHIDGLGGLFLAILGVIGFLTGVYSIGYMRHEV
                     AHGELSPVTLCDYYGFFHLFLFTMLLVVTSNNLIVMWAAIEATTLSSAFLVGIYGQRS
                     SLESAWKYIIICTVGVAFGLFGTVLVYANAASVMPQAEMAIFWSEVLKQSSLLDPTLM
                     LLAFVFVLIGFGTKTGLFPMHAWLPDAHSEAPSPVSALLSAVLLNCALLVLIRYYIII
                     CQAIGSDFPNRLLLIFGMLSVAVAAFFILVQRDIKRLLAYSSVENMGLVAVALGIGGP
                     LGIFAALLHTLNHSLAKTLLFCGSGNVLLKYGTRDLNVVCGMLKIMPFTAVLFGGGAL
                     ALALAGMPPFNIFLSEFMTVTAGLARNHLLIIVLLLLLLTLVLAGLVRMAARVLMAKP
                     PQAVNRGDLGWLTTSPMVILLVMMLAMGTHIPQPVIRILAGASTIVLSGTHDLPAQRS
                     TWHDFLPSGTASVSEKHSER"
     gene            117299..119013
                     /gene="hyfG"
                     /locus_tag="OO357_000113"
                     /pseudo
     CDS             117299..119013
                     /gene="hyfG"
                     /locus_tag="OO357_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416982.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfG"
     gene            119023..119568
                     /gene="hyfH"
                     /locus_tag="OO357_000114"
     CDS             119023..119568
                     /gene="hyfH"
                     /locus_tag="OO357_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 subunit H"
                     /protein_id="HCR5885205.1"
                     /translation="MLKLLKTIMRAGTATVKYPFAPLEVSPGFRGKPDLMPSQCIACG
                     ACACACPANALTIQTDDQQNSRTWQLYLGRCIYCGRCEEVCPTRAIQLTNNFELTVTH
                     KADLYTHATFHLQRCSRCERPFAPQKTVALAAELLAQQQNAPQNREMLWAQASVCPEC
                     KQRATLINDDTDVPLVAKEQL"
     gene            119565..120323
                     /gene="hyfI"
                     /locus_tag="OO357_000115"
     CDS             119565..120323
                     /gene="hyfI"
                     /locus_tag="OO357_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 catalytic subunit HyfI"
                     /protein_id="HCR5885206.1"
                     /translation="MSPVLTQYVSQPITLDEQTQKMKRHLLQDIRRSAYVYRIDCGGC
                     NACEIEIFAAITPVFDAERFGIKVVSSPRHADILLFTGAVTRAMRMPALRAYESAPDH
                     KICVSYGACGVGGGIFHDLYSVWGGSDTIVPIDVWIPGCPPTPAATIHGFAVALGLLQ
                     QKIHAVDYRDPTGVTMQPLWPQIPPSQRIAIEREARRLAGYRQGREICDRLLRHLSDD
                     PTGNRVNTWLRDADDPRLNSIVQQLFRVLRGLHD"
     gene            120316..120729
                     /gene="hyfJ"
                     /locus_tag="OO357_000116"
     CDS             120316..120729
                     /gene="hyfJ"
                     /locus_tag="OO357_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416985.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 4 assembly chaperone HyfJ"
                     /protein_id="HCR5885207.1"
                     /translation="MTEECGEIVFWTLRKKFVASSDEMPEHSSQVMYYSLAIGHHVGV
                     IDCLNVAFRCPLTEYEDWLALVEEEQARRKMLGVMTFGEIVIDASHTALLTRAFAPLA
                     DDATSVWQARSIQFIHLLDEIVLEPAIYLMARKIA"
     gene            120759..122588
                     /gene="hyfR"
                     /locus_tag="OO357_000117"
     CDS             120759..122588
                     /gene="hyfR"
                     /locus_tag="OO357_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HyfR"
                     /protein_id="HCR5885208.1"
                     /translation="MAMSDEAMFAPPQGITIEAVNGMLAERLAQKHGKASLLRAFIPL
                     PPPFSPVQLIELHVLKSNFYYRYHDDGSDVTATTEYQGEMVDYSRYAVLLGSSGMAEL
                     RFIRTHGSRFTPQDCTLFNWLARIITPVLQSWLNDEAQQVALRLLEKDRDHHRVLVDI
                     TNAVLSHLDLDDLIADVAREIHHFFGLASVSMVLGDHRKNEKFSLWCSDLSASHCACL
                     PHNMPGDSVLLTQTLQTRQPTLTHRADDLFLWQRDPLLLLLASNGCESALLIPLTFGN
                     HTPGALLLAHTSSTLFSEENCQLLQHIADRIAIAVGNADACRRMTDLQESLQQENHQL
                     SEQLLSNLGVGDIIYQSQAMEDLLQQVDIVAKSDSTVLICGETGTGKEVIARAIHQLS
                     PRHDKPLVKINCAAIPASLLESELFGHDKGAFTGAINTHRGRFEIADGGTLFLDEIGD
                     LPLELQPKLLRVLQEREIERLGGSRTIPVNVRVIAATNRDLWQMVEDRQFRSDLFYRL
                     NVFPLELPPLRDRPEDIPLLAKHFTQKMARHMNRSIDAIPTEALRQLMSWDWPGNVRE
                     LENVIERAVLLTRGNSLNLHLNVRQSRLLPTLNEDSALRSSMA"
     gene            122589..122771
                     /locus_tag="OO357_000118"
     CDS             122589..122771
                     /locus_tag="OO357_000118"
                     /inference="COORDINATES: protein motif:HMM:NF014950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase"
                     /protein_id="HCR5885209.1"
                     /translation="MLHPTTPENDEEERQRIVQVLRETNGIVAGPRGAATRLGMKRTT
                     LLSRMQRLGISVREVL"
     gene            122793..123633
                     /locus_tag="OO357_000119"
                     /pseudo
     CDS             122793..123633
                     /locus_tag="OO357_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311381.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter"
BASE COUNT        30750 a        31776 c        30290 g        30817 t
ORIGIN      
        1 tactttggct gacatttttt tcccttaaat tcatctgagt tacgcatagt gataaacctc
       61 tttctcgcaa aatcgtcatg gatttactaa aacatgcata ttcgatcaca aaacgccata
      121 gttaacgtta acatttgcga tattcatcgc atttatgaaa gtaagggact atatttttat
      181 aaaagttaac gttaataatt caccaaattt gcttaaccag gatgattaaa atgacgcaat
      241 ctcgattgca tgcggcgcaa aacgcactag caaaacttca tgagcaccgg ggtaacactt
      301 tctatcccca ttttcacctc gcgcctcctg ccggatggat gaacgatcca aacggcctga
      361 tctggtttaa cgatcgttat cacgcgtttt atcaacatca cccgatgagc gaacactggg
      421 gaccaatgca ctggggacat gccaccagtg acgatatgat ccactggcag catgagccta
      481 ttgcgctagc gctaggagac gataatgaca aagacgggtg tttttcaggt agtgctgtcg
      541 atgacaatgg tgtcctctca cttatctaca ccggacacgt ctggctcgat ggtgcaggta
      601 atgacgatgc aattcgcgaa gtacaatgtc tggctaccag tcgggatggt attcatttcg
      661 agaaacaggg tgtgatcctc actccaccag aaggaatcat gcacttccgc gatcctaaag
      721 tgtggcgtga agccgacaca tggtggatgg tagtcggggc gaaagatcca ggcaacacgg
      781 ggcagatcct gctttatcgc ggcagttcat tgcgtgaatg ggccttcgat cgcgtactgg
      841 cccacgctga tgcgggtgaa agctatatgt gggaatgtcc ggactttttc agccttggcg
      901 atcagcatta tctgatgttt tccccgcagg gaatgaatgc cgagggatac agttaccgaa
      961 atcgctttca aagtggcgta atacccggaa tgtggtcgcc aggacgactt tttgcacaat
     1021 ccgggcattt tactgaactt gataacgggc atgactttta tgcaccacaa agctttttag
     1081 cgaaggatgg tcggcgtatt gttatcggct ggatggatat gtgggaatcg ccaatgccct
     1141 caaaacgtga aggatgggca ggctgcatga cgctggcgcg cgagctatca gagagcaatg
     1201 gcaaacttct acaacgcccg gttcacgaag ctgagtcgtt acgccagcag catcaatctg
     1261 tctctccccg cagaatcagc aataaatatg ttttgcagga aaacgcgcaa gcagttgaga
     1321 ttcagttgca gtgggcgctg aagaacagtg atgccgaaca ttacggatta cagctcggca
     1381 ctggaatgcg gctgtatatt gataaccaat ctgagcgact tattttgtgg cggtattacc
     1441 cacacgagaa tttagacggc taccgtagta ttcccctccc gcagcgtgac acgctcgccc
     1501 taaggatatt tatcgataca tcatccgtgg aagtatttat taacgacggg gaagcggtga
     1561 tgagtagtcg aatctatccg cagccagaag aacgggaact gtcgctttat gcctcccacg
     1621 gagtggctgt gctgcaacat ggagcactct ggctactggg ttaacataat atcaggtgga
     1681 acaacggatc aacagcgggc aagggatccg cgtcactctt cccccttcac gaccttcaat
     1741 aatatgcaat gcagcttccc gcccgataat gtcatgtgga agctgaattg tggtcagcgg
     1801 cggtaaaaac agatgcccga cgccaaccag attatcaaag cccattacag cgacatcctg
     1861 cgggatacgt acccccttcg ccaaaagaac ctgataagcc acaaaggctg cgcgatcgtt
     1921 accacatatc agaacatcaa aatctggttt gcccgatttg aagtgagcat tgagtaaact
     1981 tgcgagatcg gtgtagtgat catcacctgt tgccatgtga aattgtttca cctcagccag
     2041 atctcgtcca gcatcacgcc aagcctgctc aaatccctgc cgacgatacc ctgttgccaa
     2101 cgcactttcc ggtagccaga agcataatgg ttgacgatag cccgccgcga gcaaatgctg
     2161 tgttgattca tattgtgcag tgtaatcatc agggatataa ctgggtaacg ctgggtcatc
     2221 cgccacacag ttcgccaata caatattttc accatacaga gactcaggca gcgtgatatg
     2281 tcgcagcccc attgtagtat agataatgcc atccggacgg tgggcaagca gctgacgtgc
     2341 cgcgcgggca gcgtcatctt cagaaaaaat attgattaaa aaactattcc agccgaactc
     2401 gctggcggtt tgctcaatgg caagcagaat atcaacagag aaaggagtgg tagccgtgtc
     2461 ctgcgccagc acggcgagag tcgacggctt acgtccttga gcgcgcatct tacgggcgga
     2521 aagatcagga acataattca gggtctggat tgcctgcaat acgcggtcac gcgttgcagg
     2581 acgcacagat tctgcattat gcatcacccg ggagactgtc atcatcgaca ctcccgccag
     2641 gcgtgcgaca tcctttaatg aagccatacc caagccgttt gccgtaaaac gggcactgta
     2701 gcagaaacag acgtcactgg cgagatccaa cgccctatca cctgacacag caatacaata
     2761 aaaaataaca ataattcccg gacaattgtc cccaattccg cctctgttct cgccaacgag
     2821 taaccatata ggcaagtaag taaaacatgc caactggcat cgccaacctg gagaagaaaa
     2881 tataaatatg gatcattgtg ctgccactgt atcccgacat cagcccggaa attattgcga
     2941 ttgctatcgg ttcaggtgca attggctgca cgatcgttac ggactcgctt ttctggctgg
     3001 tgaagcaata ttgcggcgct acgctcaatg aaacatttaa atactatacg acagcgacat
     3061 ttatcgcttc agtcgtcgct ctggcgggca cattcctgct gtcatttatc atctaagcgc
     3121 aaagagacgt actatggaaa acgctaaaat gaactcgctc atcgcccagt atccgttggt
     3181 aaaggatctg gttgctctta aagaaaccac ctggtttaat cctagcacga cctcattggc
     3241 tgaaggttta ccttatgttg gcctgaccga acaggatgtt caggacgccc atgcgcgctt
     3301 atcccgtttt gcaccctatc tggcaaaagc atttgctgaa acagctgcca ctggggggat
     3361 tattgaatca gaactggttg ccattccggc tatgcaaaaa cggctggaaa aggaatatca
     3421 acaaccgatc agcgggcaac tcctactgaa aaaagatagc catttgccca tttccggctc
     3481 tataaaagca cgcggcggga tttatgaagt cctggcacat gcagaaaaac tggctctgga
     3541 agcggggttg ctgacgcttg atgatgacta cagcaaactg ctttctccgg agtttaaaca
     3601 gttctttagc caatacagca ttgctgtggg ctcaaccgga aatctggggt tatcaatcgg
     3661 cattatgagc gcccgcattg gctttaaggt gacagttcat atgtctgctg atgcccgggc
     3721 atggaaaaaa gcgaaactgc gcagccatgg cgttacggtc gtggaatatg agcaagatta
     3781 tggtgttgcc gtcgaggaag gacgtaaagc agcgcagtct gacccgaact gtttctttat
     3841 tgatgacgaa aattcccgca cgttgttcct tgggtattcc gttgctggcc agcgtcttaa
     3901 agcgcaattt gcccagcaag gccgtatcgt cgatgctgat aaccctctgt ttgtctatct
     3961 gccgtgtggt gttggcggtg gtcctggtgg cgtcgcattc gggcttaaac tggcgtttgg
     4021 cgatcatgtt cactgctttt ttgccgaacc aacgcactcc ccttgtatgt tgttaggcgt
     4081 ccatacagga ttacacgatc agatttctgt tcaggatatt ggtatcgaca accttaccgc
     4141 agcggatggc cttgcagttg gtcgcgcatc atgctttgtc gggcgggcaa tggagcgtct
     4201 gctggatggc ttctataccc ttagcgatca aaccatgtat gacatgcttg gctggctggc
     4261 gcaggaagaa ggtattcgtc ttgaaccttc agcactggcg ggtatggccg gacctcagcg
     4321 cgtgtgtgca tcagtaagtt accaacagat gcacggtttc agcgcagaac aattgcgtaa
     4381 tgccactcat ctggtgtggg cgacgggagg tggaatggtg ccggaagaag agatgaatca
     4441 atatctggca aaaggccgtt aataacgttt caacgcaaca tcgcaatcct ttccctgggt
     4501 gagcgatgct gccgatggcg cagacttaag atccccggtc ttacccgcta taaccccctc
     4561 cttttatcat cacccaacgc ctttcgctgt aaacggcggt ttcgcaaacc aaaccaaaac
     4621 cgtaagtaag ataaaagcaa tagccgccat acggaaaatc tcatttgcag aaatagaaag
     4681 tgactgctgg gttatttcat tattaatttc attaagaact cctgaaagcg aaccatagta
     4741 tttatccata atttgtgacg atgaattaaa cacggggtta aattgatcga tggttgctgt
     4801 caactgacta tggtgtaacg attcgcgtcg tccccacagc gtcattgtca acgacgtacc
     4861 aactgatcct gacaaggtac gaaaaaaatt actcatactc gaggcattgg caaatttatt
     4921 atctggcaag cctgaaaacg aaatcgttgt taagggtaaa aagaaacagg caacggcgaa
     4981 tccctggaaa aactgtggca aaatgatgcc tgtaaaatca atcgttggca taaatgtcac
     5041 agaacgccag taatagcaaa ccgcatacat caaaaaactg aatgtcacta acacccgcat
     5101 gtctatttta ttgccataac gtcctatcaa aggtgaaatc aatagtggca tgatgccgat
     5161 gggcgcataa gcaagcccgg cccatatcgc attatacccc atcgttttct ggagtaactg
     5221 cggcataagg acgatcgctc cagagtaaaa taaatacgcg caggtgatac tcacaatacc
     5281 aatggtgaaa ttacgggact taaacaaact gagatcaaga atcgggttct ctgaggtcga
     5341 ttcccaaatg actaaagaga tcagagaaat aactgatact actgttaata ttattattgt
     5401 actcgagttg aaccaatcca gatcgcgccc tttatcaagc ataatttgca agccaccaac
     5461 accgagcact aacagggtca gtcttggtag attcattttt accggtgaag tttcagtttc
     5521 tcttccttta agtaaggtta agcataatgt caggacgata atccccatag ggacattgat
     5581 taaaaatatc caaccccagc taaagttatc gcaaatatag ccgcccaata tcggcccaca
     5641 tatcggagcg ataatcacgg tcattgacca taatgccaga gcaaatgttc ttttttctgg
     5701 tggataattc cttaataata aactctgtga cagtggaatt aacggccccg ccattaatcc
     5761 ctgaacgact ctaaaaaata tcagcacatc aagattggtc gataaactac acattaagga
     5821 agacagcgaa aaaaaagtga ctgaaagtaa aaataacctt aattcgccta ttctttgtgc
     5881 cagcctgccg gtaacaggga tcgcaatggc atttgctacg ccaaacgagg tgataaccca
     5941 ggtgccttcg tctgttgatg ctcccagaaa gccagatatt gtcggtattg cgacgttaga
     6001 aatagtggaa tccaacattt gcataaacgt cgctaatgac aatgcaatag taacgcacca
     6061 taacgtccca ccggttaatg gtgccggagt tgatttagtg attgccatta aagttgtcca
     6121 ttatgcgaaa taatgttgct aatttctttt tctatcggac tggtatcgat aactaaagcc
     6181 ttactggtat aagcaggcat ggaggtcacg gttgaagcca gatcaggcat ctcagcaatg
     6241 tcttcgtcct tcgtatcaat agttgcagtc atcgataaac caatgcgcaa ggggtgttcc
     6301 atgagttctt ttggatcaag agaaacttca accggtacac gctgaacgat tttgatccag
     6361 ttccctgtcg cattttgtgc aggtaataag gagaacgcat tgccggttcc catattgatc
     6421 cctgtcaccc gaccatgaaa cacaacattt tcaccataaa gatcgctgat aatattgact
     6481 gattgaccaa tccgtacatc cgtgagttgt gtttctttaa agttggcatt aacccacatt
     6541 tgacgtgccg gtacgacagc cattaacgat tgtccggaac tcactgtttc gccgacctga
     6601 acacttcgct gggcaatata gccagtaacc ggactcctta tatccgtacg tttaagcacc
     6661 aaccaggctt ctttggttgc atccgccgct tcaactactt gtggctgacg gtttaatggt
     6721 gtgttcatta ctaaagcttt attcgcttta taagcctgga tagcggcatt cagtgccgct
     6781 ttgctactta ttaacgtatc tttggtatgc tccagcgttt cttttgaaat aaccccctgc
     6841 ttcgctaaag gcacacgacg gttataatct tctaaagatt gttgatactg aatacgtgct
     6901 gaagcgactt cggcactgta ttgtttatcc tgtaagtaca gtttattcgt ttgccgaaca
     6961 atatttgcca gattattttt agctttattg agtgcgatag tggcatcagt tttatccagt
     7021 gaaactaaaa tgtcaccctg tcgaacgtag ttcgtatctt tatgattaac tacagtgaca
     7081 ctacctgaga cttgtgcaga aattggatct gcattccccg tgacataggc gtcatctgta
     7141 ctaatcatgt cttctaattc cattgaccaa taggcatagg cacctgaaaa cgcaataaat
     7201 aaaactatca ccaataaaga aaagtatttt tttctgttag actgtttttt atttgaatta
     7261 atctgttcca ctattatctc tcatttcgca tagatgaaat ttatgaattg aatgaaaaga
     7321 gtattcattc ttcatatcaa acacagtgca tgtgttgcac attatcccag gaacgttgga
     7381 ggcctaattt aaatgaggtg caaaaaaaac aacagaggac taaaccgtgg cttttgcaat
     7441 acaattctta cgcctgtagg attagtgaga agacttatag tgctaacttg aaactataaa
     7501 tcatcggtat aatccctgat tttattgttg acatttcatt tatgccgact atttatatgg
     7561 tatacttgtc gaattatctt aaaggaaact cagattttct tatttttatt gagaaaatga
     7621 gatgacgcct tatgtctgta ttactacagg gagaagggag atgcttcatt gcaaagggaa
     7681 taatctatga acgcaataat tattgatgac catcctcttg ctatcgcagc aattcgtaat
     7741 ttattgatca aaaacgatat tgaaatctta gcggagttaa ctgaaggcgg aagcgccgtt
     7801 cagcgggtgg aaacacttaa gcctgatatc gtcatcattg atgtcgatat ccccggagtt
     7861 aacggtatcc aggtgttaga aacgctgagg aagcgccaat atagcggaat tattattatc
     7921 gtctccgcta aaaatgacca tttttacggg aaacattgtg ctgatgctgg cgctaatggt
     7981 ttcgtgagta aaaaagaagg catgaacaat atcattgcgg ctattgaagc tgcaaaaaat
     8041 ggctactgct atttcccctt ctctctcaac cggtttgttg gaagtttaac gtccgaccag
     8101 caaaaactcg actccttatc gaaacaagaa attagtgtca tgcggtatat tcttgatggc
     8161 aaggataata atgacattgc ggaaaaaatg ttcatcagca acaaaactgt cagcacgtat
     8221 aaaagtcgcc tgatggaaaa attagaatgt aaatcactga tggatcttta cacattcgcg
     8281 caacgtaaca aaatcggcta atcacatgaa gtttttaccc tatttttttc ttctctgttg
     8341 tggtctttgg tcgaccataa gtttcgcaga cgaagattac atcgaatatc gtggcatcag
     8401 tagtaacaac cgtgtcacac ttgatccagt acgtctgagc aacaaggaat tacgttggtt
     8461 agcgagcaaa aaaaatcttg tgattgcagt acacaagtcc caaacggcta ccttgttgca
     8521 taccgattcg cagcaacagg ttcgtggtat taatgctgat tatttaaatc ttttaaaaag
     8581 agcgttaaat atcaaattaa cactccggga atacgcagat catcaaaaag caatggacgc
     8641 gcttgcagaa ggtgaagtcg atatagtgtt atcacattta gttacttcgc cgcctcttaa
     8701 taatgacatt gctgcaacca aaccattgat aattaccttt ccggcgctgg taaccaccct
     8761 tcacgactca atgcgaccgc ttacctcacc aaaaccagta aatattgctc gggtagcaaa
     8821 ttacccccca gacgaggtaa ttcatcaatc atttccaaaa gcaacaatta tctcttttac
     8881 aaatttatat caggcattag catccgtctc agctgggcac aatgattact ttattggtag
     8941 taacatcatt accagcagta tgatttcccg ctatttcact cactccttaa atgtagtgaa
     9001 atattataac tcgccgcgtc aatataattt tttcttgacc agaaaagaat ctgtcattct
     9061 taatgaagta ctcaatagat ttgttgatgc tttaacaaat gaagttcgct atgaagtatc
     9121 acaaaattgg cttgatacag gaaacctggc ctttctgaac aaaccattag aactcactga
     9181 acatgaaaaa cagtggatta agcagcatcc caatttaaag gtgctggaaa atccttactc
     9241 gcccccctat tctatgacgg atgaaaatgg ctcggttcgg ggcgttatgg gggacattct
     9301 taatattatt accttgcaaa caggtttaaa tttttctccg atcaccgttt cacacaatat
     9361 ccatgctgga acacaactta gccccggagg atgggatata atacctggcg ctatttatag
     9421 tgaagatcga gaaaataatg ttttatttgc tgaagccttc ataacaacgc cttacgtttt
     9481 tgtcatgcaa aaagcgcctg acagtgaaca aacattaaaa aaaggaatga aagttgccat
     9541 tccatattat tatgagctgc attcgcaatt aaaagagatg tatccggagg ttgaatggat
     9601 acaggtcgat aatgccagcg ctgcatttca caagattaag gaaggtgaac ttgatgctct
     9661 ggtcgcgaca cagctaaatt cgcgttacat gatcgatcat tactatccta atgaacttta
     9721 tcattttctt attcctggcg ttccgaatgc atcgctttcg ttcgcttttc ctcgcggaga
     9781 accggaactt aaggatatta ttaataaagc actgaatgca attcccccaa gcgaagttct
     9841 gcgcctgacg gaaaaatgga ttaaaatgcc caatgtgacc attgacacat gggacctata
     9901 tagcgagcaa ttttatattg ttacgacatt atccgtttta ttagttggca gtagcctttt
     9961 atggggattc tacctgttac gctcagttcg tcgtcgtaaa gtcattcagg gtgatttaga
    10021 aaaccaaata tcattccgaa aagcactctc ggattcctta ccgaatccaa cttatgttgt
    10081 aaactggcaa ggtaatgtca ttagtcataa tagtgctttt gaacattatt tcactgcgga
    10141 ttactacaaa aatgcaatgt taccattaga aaacagtgat tcacccttta aagatgtttt
    10201 ttctaatgcg catgaagtca cagcagaaac gaaagaaaat cgaacaatat acacacaggt
    10261 atttgaaatt gataatggca tcgagaaaag atgcattaat cactggcata cattatgcaa
    10321 tcttcctgca agtgacaatg cagtatatat ttgtggttgg caagatatta ctgaaacgcg
    10381 tgatctaatt aatgcactcg aggtagaaaa aaataaagcg ataaaggcta ccgtagcaaa
    10441 aagtcagttt ctggcaacga tgagtcacga aataagaaca ccaataagct ctattatggg
    10501 cttcctggaa cttctgtcgg gttctggtct tagcaaggag caacgggtgg aggcgatttc
    10561 acttgcctac gccaccggac aatcactcct cggcttaatt ggtgaaatcc ttgatgtcga
    10621 caaaattgaa tcgggtaact atcaacttca accacaatgg gtcgatatcc ctactttagt
    10681 ccagaacact tgtcactctt tcggtgcgat tgctgcaagc aaatcgatcg cattaagttg
    10741 cagcagtaca tttcctgatc attacctggt taagatcgac cctcaggcgt ttaagcaggt
    10801 cttatcaaat ttactgagta atgctctcaa atttaccacc gagggggcag taaaaattac
    10861 gacctccctg ggtcacattg atgacaacca cgctgttatc aaaatgacga ttatggattc
    10921 tggaagtgga ttatcgcagg aagaacaaca acaactgttt aagcgctaca gccaaacaag
    10981 tgcaggtcgt cagcaaacag gttctggttt aggcttaatg atctgcaaag aattaattaa
    11041 aaatatgcag ggcgatttat cattagaaag tcatccaggc ataggaacaa catttacgat
    11101 cacaatcccg gtagaaatta gccagcaagt ggcgactgtc gaggcaaaag cagaacaacc
    11161 catcacacta cctgaaaagt tgagcatatt aatcgcggat gatcatccga ccaacaggct
    11221 attactcaaa cgccagctaa atctattagg atatgatgtt gatgaagcca ctgatggtgt
    11281 gcaagcgcta cacaaagtca gtatgcaaca ttatgatctg cttattactg acgttaatat
    11341 gccgaatatg gatggttttg agttgactcg caaactccgt gagcaaaatt cttccttacc
    11401 catctggggg cttacagcca acgcacaggc taacgaacgt gaaaaagggt taagttgcgg
    11461 catgaactta tgtttgttca aaccgttgac cctggatgta ctgaaaacac atttaagtca
    11521 gttacaccag gttgcgcata ttgcacctca gtatcgccac cttgatatcg aagccctgaa
    11581 aaataatacg gcgaatgatc tacaactgat gcaggagatt ctcatgactt tccagcatga
    11641 aacgcataaa gatctacccg ctgcgtttca tgcactagaa gctggcgata acagaacttt
    11701 ccatcagtgt attcatcgca tccacggtgc ggctaacatc ctgaatttgc aaaagttgat
    11761 taatattagc catcagttag aaataacacc tgcttcagat gacagtaagc ctgaaattct
    11821 tcagctgctg aactctgtta aagaacacat tgcagagctg gaccaggaga ttgctgtttt
    11881 ctgtcagaaa aatgactaaa ttgcggctcc cacaatgttc taatgtggga gctatttacc
    11941 agcacatctt acatttatga tgagaactcc tggcgaattt gttccccatg ctggtcgagc
    12001 gttgccgctc ccggcataac atgcgggtcc gcgcagccgc tgatttttat cggattaccc
    12061 ggcatcatta ttcctccggc ttcaatcaac atatttctcg cctgagtttg tggcaaatta
    12121 atggcctcag ccacacttaa taacggcgcg acgggcacac caacttcatg tattttggct
    12181 aaccaaactt cagctgcctg cgtttttaac gttcgctcaa tatattgttt aagaatagcc
    12241 tggttttgta cgcgtaaaat attgctgcta aatcggggat cattaaccag ttccgtaagc
    12301 tccagtgcct ggcataacgc agaaaaaagc ttgtcattac cacaacaaat cgtaatcggc
    12361 ttatcctgag tatcgaaaac atcaaaaggt gccatgtagg gatggcgatt tcccaggcgt
    12421 tgtggtgact tccctgtcgc aatatatgcc atcagaccat gctccagaaa actcagcgtg
    12481 gcatcaaaca tcgctatatc gacatgcgcc cctctctggc tcttttcgcg gccataaagt
    12541 gcactcacta ttccgctgaa taaatagaca ccgccgcata gatccgcaag agaggtacca
    12601 acacgcactg gcggagcatc agggtaccct gtttccatca ttatcccact cattgcctga
    12661 atgatggtat cgtaggcagg agcatctttt agcggaccgg tatgtccgaa acctgacgat
    12721 gaagcatata tgaggcgcgg gttgatttct tgtaaccttt cccatgaaaa ccccagtttt
    12781 tccattgtac ctaggcgaaa attctcagct aatacatcag cttgtttgag catatttata
    12841 aatatacttt tatcgtgatc attctttaaa tcaagaacca cactctcttt gccatgatta
    12901 ataaaactgt aatagagtga ctgtccatcc acatagggac caaatgtgcg ggtatcatca
    12961 ccatgacctg gtggctcaac tttaattacc cttgcgccca tattataaag aagttgagtt
    13021 ccgaaaggtc cattaaggac atgtgtcata tcgataacta ataagccttc aaacggccct
    13081 ttgctttcat tatttgtcat ttgcttatgc cttgaaaaaa gccgaaggtg aaaagacact
    13141 tcggcattta tgtaaaatca ataacatgat taattatgaa accagtcgac tgacataaat
    13201 ccacaaggga gccgtgacga caaagcccag tacgctcaca gccaatgacg ctgtaccagt
    13261 acgggtgtag acattaaacc ggctggcaat aatgattccg gagaatgccg gcggtaatgc
    13321 gcctgccagt accatcatct gcaaatgttc gctgttcaaa tgacatgcca taccaacgag
    13381 aagcagcgcc agtggcatca gaatcaactt caggaaggtg ttataagcaa tttcggcact
    13441 gaactcgaat ttatgtgcag ccagagtcaa ccccgcagcg aataccgcta cccctgagtt
    13501 agccttcgca atcagattaa aggttgggtc ccatgctgcc ggaattttta ccccaaccag
    13561 caccaggatc gttgccagaa caggtgccca tactactggc tcctttgccg cagaaattaa
    13621 tgcgctcaga ttactattct tcttaccatc cgctcctgag gaaggattca gcaaatacag
    13681 accaatagga atagtaattg cgttaacaat aatagaaata attgctacca ctaaacctgt
    13741 tgatacggaa tcaccataaa taggatcgag aactgcaaac cccaggaatc caatggtagg
    13801 tgaacccgca attaatgcac atacagctgc ttctgcatgg gtacgtttaa aaaatttgta
    13861 gcaaccaaac caggagaaaa agaaacatcc gacaataacc acaagtgata caagggtcag
    13921 acgagtgtcc gcaaaaatca tttccctgtt tgcccgggta atagaaacaa atagagccgc
    13981 aggaagagcg tagtttaata ccagtttatt aaatgcgcgg gcttgatctt ctgaaaaagt
    14041 ttctcgtctg ccgctaaaat aacccaacag catgattacg ataataggca ataaatcgcc
    14101 aataaaaaat gttagcataa tagtatttcc gataagggtc ccctcaccat cgcataatga
    14161 gttggcaaga ggagacgatg tcagtattta attaccagcg acttgttttg ggttaagttt
    14221 ggtaatatgg ccactttcag ttcctgctgc agggtcgata accacattaa taatggtcgg
    14281 tttgcgcgac tgaataccgg tggttaaagc atgacgaagt tcatctgtcg tggtgacgtt
    14341 atagccaacg ccacgaaacg catccattaa tttgtcatac cttgcatggt gcaacagatc
    14401 cgttggtgat ggtgcaccag cgccactgag atcaacaccg tctcctctgt agatgccgcc
    14461 attattaaaa ataacgatcg tcaccggcag gttatatcga caaatcgttt caatttccat
    14521 cccactgaaa ccaaaagcac tatcaccttc aatggcgaca accggagaac cagaagtcac
    14581 gctagcaccg atggcatagc ccataccgat gcccatgaca ccccaggtac cacaatccag
    14641 acgacgacgt ggtttataca tatcaataat atttcgtgca ttatccaggg tatttgcacc
    14701 ttcattaact aaataaatat cctggttctc gcgcaatacg tcgcgcacag cacttaatgc
    14761 attaaagtaa tttaatggtt gagtatctgt acttaatttt tcatgcattt tttgtgcatt
    14821 ttgctgcttg tggatattta aaatatcgcg ccataccagt ggagtcgtaa atgtgttttg
    14881 tttcagttct gccagcatac cttgcatact ggatgcaata tcaccaacga ctggcacagc
    14941 aatggggcgg ttgctgtcaa tttcctgcgg ttcaatatcc agttgaataa actgtgtatc
    15001 tgccgcccat ccttttttac cgtgtgccaa taaccaattc agtcgtgcac caacaagcat
    15061 gacaacgtca gcatttgcca gggcaaacga acgcgcagct gccgcagaaa gtggatgcgt
    15121 atcttcaagg atccctttcg ccatagacat tggcaggaat ggaatctgag cactttcaat
    15181 aaattcacga agctgttcat cagcttgtga atacgccgcg cctttgccaa ggataattaa
    15241 tggccgttca gcttttgcta aaagcgaaat tgcgctagtg actgacttcg ggcatggcaa
    15301 taatgctggc gacggatttt caactttaac aatcgtggtt aacgcttcgt ctttttccat
    15361 cgtcgcggcc aggacatttg ctggcaaatc aagataaact ccgccagggc gacccgatac
    15421 agagacccgg atagcgcgtg ccaatgcaat gccaagatcc tgcggctgat taacgcgaaa
    15481 tgctgctttg gcatacggtt ttgccgcatt catttggtcc agctcttcat aatcaccttg
    15541 ctgtaggtcg acgatcgcgc ggtcgctgga gccgctaatc atgataatcg gaaaaccatt
    15601 taccgttgcg ttggccaatg cggtcaaacc attgaggaat cctggcgcag aaactgtcag
    15661 gcagatcccc ggtttttggg taagaaaacc gcttgccgca gcggcatagc ctgccgactg
    15721 ctcatgacga aaaccaatat aacgaatgcc ttccgcctgg gcatggcgtg ccatatccgt
    15781 cacaggaata cctacaacac cataaatagt gtcaatatta ttctgtttta atgcttcaac
    15841 gatgatatgc ataccatctg tcatttgaag ttgatctgac atgattaata acctcgaaat
    15901 ttttataaat tgggagccag aagcccccgt taatatcaga tggcgtggtt ttgcttcatt
    15961 gcagcaattt catcgtcgct ataacccagc tcctgcaata cagcagcggt atgttcacct
    16021 aatagcggcg cagctttaat atccggcgta aaggcagaga atttcattgg acaaccaacg
    16081 gtcagatatt ttccacgcaa cggttgttcc acttcaacaa cactgccact ttggcgcaaa
    16141 gagggatcaa gtgaaatttc tttcatactt aaaaccggtg cacaaggaat atcaaactga
    16201 gtcaaatagg ccaccgcttc atgtttatca atagtgacag tgtatttttc gatttcagca
    16261 aaaatatcga aaatatgtgg ctgtcgggca tgggctgtac tgtatgccgg atcggtaatc
    16321 cattctggtt tgccgatggc tttacaggtg ttttcccagt tttgctcctg aatagtgaaa
    16381 taaatatagg cgttaggatc ggtttcccag cctttacatt tcaggatcca gccaggctga
    16441 ccgccaccac ctgcattacc accgcgggga actgcatcac caaatgtacc attcggatac
    16501 tgcgggtatt cttccagata acccaattta tcgagacgct gctggtcacg taatttcacg
    16561 cggcaaaggt tcaatacggc atcctgcatt gacatggtga ctcgttgccc acgccccgtt
    16621 ttttcgcgat gcagcaaagc agcaagtaaa ccgatcagca aatgcattcc ggtgttgctg
    16681 tcacccaacg ctgcagcgct taccagcggc ggaccatccc aaaaacccgt agtggatgcc
    16741 gcgccacccg ctgcctgagc aacgttttca taggctttta cattcacata aggcgaacac
    16801 tcatcaaacc ctttgatcga accaaaaatc agacgtggat tgatttcttg aatatgctcc
    16861 caggtgaagc ccatgtgatc aatggcccct ggatgaaagt tctcgactaa gatatcagct
    16921 tcgcggatca gcttttccat tacctctttg ccttccgctg ttttggtatt taactcaata
    16981 gaacgtttgt tactgttaag catggtgaag taaagcgcat cgatatcagg aatatctcgc
    17041 agttggtgac gcgttacgtc accaacgccg ggacgttcaa ttttaataac gtcagcgcca
    17101 aaccaggcca gcatttgagt acaagatggg ccagattgca caccggtgaa atcgagaact
    17161 ttaattcctt gaagtggagt tgacatagca atacctatta gttaattaaa tatgtgttaa
    17221 tttagcgttt atttaaggca taacgccctc tataaataat ctgcaaaata tcttcgtagc
    17281 ggcactcgat atttttaaat tcctgtatca agtactgaaa attattttat cgccacttcc
    17341 aattcctttc tgcatggtaa gtagtgagga cgaaaagaag aatatgataa atattaattc
    17401 gtggtaatca gagagagtcg cgacatttgt gatgttattc ttacaactgt aagaagcgcc
    17461 ggtgatttac aagtaagagt atgattacgc tttttaaagc gtaccataaa gcataacata
    17521 ataaatgatt tgtgatgaca cgggattatt gcggtttact ttgaggaaaa taaaatgcgt
    17581 gtttatagaa ccgcgagcgc caaataattt tatgaccaca acctgtcgta ttgtggtcat
    17641 aaaaaattgt cggctggagg gagttaaatc ctttacctca atatactgcc tgtcagcatt
    17701 agttcacaaa cagtgcttcg ctatcgacga tgattccatc ttccgcacct ttaagcgcca
    17761 ggtttgcctc gtagtattgc tttttatcaa gcagtttctg cgcatcagca agtgcattac
    17821 gagtctgttt aagcggcatc agatattggt tttccattac gccaacaccg gccaggcgaa
    17881 gctcttccat tgcgcccttt ttatcccctt tcgccatttt ttcattggca atttttatcg
    17941 cggcttcttt ttcgggtgtt gctacataac tctcagaaat tccgacagat gcattaatga
    18001 caatatattg gtcatcattg agattggtct ttttacctgg cttagcgaac tttgcccatt
    18061 cggtactgtc atctgacagc aaagcggaag cttcattggt cagttctttt gccttttccg
    18121 gatcgccatg aaacagagcc aggcgcgcta cctgcacgtc acgcatcgca tataaacctt
    18181 gttcagagat acgctctgct gctgccgttt tctgggtttg ctgagtttgc tgttgtgccg
    18241 ctaccggtgc attatcagca gcccatacag ttgaagatgc cagaattgct gtgaccatcg
    18301 tagccataat taaacgtttc atacttacct cattctttaa cagttttaga aaacgccgcc
    18361 atcatattgg gtgagcatga aatgagaaat cagagagcaa cggtcatttt gaaagggtta
    18421 tcttacaatt gtagttatta tcgcaaagcg gcatttagtc ttacagttgt aggaatatgc
    18481 ctcctgtcgc agagaactat tcttatatac cacaaagaga tgtcatttat tatattgatg
    18541 taaatattga ttaattatat gtaaatacag ccataacatc gtatggttct cagtgaacca
    18601 ttttcaggat aactttctat gaaagtaaac ttaatacttt tcagcttatt tttattggtc
    18661 tctattatgg catgcaatgt ttttgcattt tccatttctg gtggtgtaag tgagaggagc
    18721 tataaagaga ctgaaaaaac atcagcgatg acgaccacac actctacaaa acttcagcca
    18781 tcacaggcga ttttgtttaa gatgagagaa gatgtgccac cattaaacct cacagaagaa
    18841 atcacgccta cttatccgac aaaggcgaat tatcttattc atcctgtgcg atagcgtctg
    18901 tcacatggca ggtgaacaat catgtctgat gctgatgtga acacctgccc aaatgagcca
    18961 tcctgaccca ctatcgcctg ccatacccca ccataaagcg aatcatcaag ccacaagccg
    19021 gggcaagaaa cataaatatc cccgtcacca gttcattacc gccagttaac cacatggtga
    19081 agtaataaat gagtgcagcg aggatcacca tgccgaagaa agaagagacc gccgatacca
    19141 cgttcagtac ctgaccgatg tagccgctta cgcagacaat acacagggcg agaaacatcc
    19201 ataaataaat catagctcaa cctcctgccg cgctaaaaaa cctgtaactt cagtcacttt
    19261 aatagtaaga caatccttaa cctctggcaa tttttgctaa gcatcaatga aaacagcacg
    19321 ttaaatgcaa cgctattgcg tgcggaaaat tttttacttt gcacgattaa taatcagtag
    19381 ctgaaagcag tcagccagtg ataaagtaga gccaccgcat tcatgctcct cgtggttatg
    19441 tcctgactag tctttacact ctttacagga accattgtcg tacatgatgg cccaaccaat
    19501 tgagtgtacc gctgtcgaca tggaatcttc tgcgatacaa caattcgtat ctacagaagg
    19561 taactatgtt tccacaatgc aaattttccc gcgagtttct acatcctcgc tactggctca
    19621 catggtttgg gcttggtgta ctctggcttt gggtacagct tccttatcct gttctctgct
    19681 ttctcggcac gcgtattggc gcaatggcgc gaccattcct gaaacgtcgt gaatctatcg
    19741 cccgtaaaaa cctggaactt tgtttcccgc agcattctgc ggaagaacgc gagaagatga
    19801 ttgccgaaaa ctttcgttca ctcggcatgg cgctggtaga aaccggcatg gcatggttct
    19861 ggcccgacag tcgcgtacgt aaatggtttg atgttgaagg gttggataac cttaaacgcg
    19921 cacaaatgca aaatcgcggc gtaatggttg tcggcgtcca ttttatgtcg ctggaactgg
    19981 gcggccgcgt gatgggactg tgccaaccaa tgatggctac ctatcgtcca cataataatc
    20041 agctgatgga atgggtgcag acccgtgggc gcatgcgctc taacaaagcg atgatcggca
    20101 gaaataatct gcgcggcatt gtcggtgcac tgaagaaagg tgaagcggta tggtttgctc
    20161 ccgatcagga ttatggtcgt aaaggcagct ccttcgcgcc gttctttgcg gtggaaaatg
    20221 tcgccacaac caatggcacc tatgttctct cccgtctctc tggcgcagcc atgttgaccg
    20281 taacgatggt aagaaaagcg gattacagcg gatatcgttt gttcatcacc ccagagatgg
    20341 aaggctaccc gacagatgaa aatcaagccg ctgcttatat gaacaagatt atcgagaaag
    20401 agatcatgcg cgcaccggag cagtacctct ggatccaccg tcgctttaaa acgcgcccgg
    20461 tgggagaatc gtcgttgtac atttaacaaa ctgctcttac tctttccctc ctcaaatcgg
    20521 ccataataac ctcatgattt ttatagcttt tttcttaagt tttttccttc gctgaatatc
    20581 taattattac cgtcagaaat taaactgtcg ccgatatgag acacttgtaa gttactaaaa
    20641 gtaaataaga aaatgcctct tgtctcgttt cagttttagg cgtacattag cgccgtctcg
    20701 agcagtttag ctcagaattc tggggtgatg cccacggtaa aatacagggc ttttaaggtc
    20761 gctttgcccg gtgtcaactc actgtatcag gtgtaatgaa gtcattcagg cgtaacagta
    20821 attacgcgga gagatgtaaa gtgaaatatt tctttatggg catttctttt atggtcatcg
    20881 tttgggccgg tacttttgcc ctgatgatct aaaagcaaac tgtgcaatga aaaacaggag
    20941 ccatcaggct cctgtttgca tttctgacga cggtgtttat tccgcgtttt cgtgaatatg
    21001 tttgctgctg acgggtagta aaccgtcggc acggaacatc gctttaatcc ctctgatggc
    21061 ctgacgaata cggtcgtggt tttcaatcag tgcaaagcga acatgcgtat caccatagtc
    21121 gccaaagcca atccccggcg agacacaaac cttcgcttcg ttaaggagct tcttggcaaa
    21181 ttccagcgat cccatggccg catatggttc cgggattttc gcccagacat acatcgaagc
    21241 cttcggcatt tcgaccatcc agcccgcttc atgcagccct ttaaccaata catcacgacg
    21301 gcgtttgtac tgttcggcaa tgtcgcgcac gcactgttga tcgccctcga gtgccgcaat
    21361 cgccgccacc tgcaacggcg taaaagtgcc gtaatcgtga tagcttttga ttcgcgccag
    21421 ggcactgacc agcgttttgt tgccgaccat aaagccgata cgccagcccg ccatgttgta
    21481 gcttttcgac agcgtaaaga actcgaccgc cacatcgcgt gcacccggca cctgcataat
    21541 tgacggcgct ttccagccat cgtagacgat atcggcatag gccaggtcat ggaccaccag
    21601 cacatcgtag catttcgcca gcgccacaac cttttcgaag aactccagtt ccacgcattg
    21661 cgcggttggg ttcgacggga agccgaggat catcatcttc ggtttcggat aactttcacg
    21721 aatggcgcgc tccagttcgt tgaagaaatc gacaccttcc accagcggca ctgagcgtac
    21781 ctgcgccccg gcaatcaccg cgccataaat atggatcggg taacttggat tcggcaccag
    21841 caccgtgtca ccatgatcca gcgtcgccag catcagatgc gccaggccct ctttcgaacc
    21901 aatagtgacg atggcttctg attccgggtc gatttcaacg tcgtagcgat cctgatacca
    21961 gcgggaaatg gcgcgacgta accgcggaat gccgcgtgaa gtggagtaac catgcgtgtc
    22021 cgggcgctgg gccacagtac ataatttttc gacgatatgc ggcggagtcg caccgtccgg
    22081 gttacccatg ctgaaatcga taatatcttc gccgcgccga cgcgcagcca ttttcagttc
    22141 agcggtgatg ttaaaaacgt agggcgggag acgatcaatg cgcgtaaagc gacgttcagg
    22201 gcgagtgtca gccataattt cctcagatta acgttagcgc ccggaccgtc cgagcgacgc
    22261 tgccacaatg gtggctgttt tgaaaatagc ctgattaatt tctgtctgtc cagcgtttta
    22321 gaacattttt atcaatggat ttaaatgagg aactccactt caaaaacagg aaatttttct
    22381 acgctaacaa gaaaatcacc accaatttta ttaacattat aatttcaata aatttaccag
    22441 ccagaagtgc attatcaaga gatacattgc taatacacca tttatctttt aatccccttt
    22501 taaatttatg gtttttcctt aaaaacggcc tgctggtcat cagccttcag gttacctatc
    22561 atagaggttt aatccttatt cagagtcacc cgtgcacgaa atattcaaca tgctgctggc
    22621 ggtcttcgat cgggcagcgt taatgcttat ctgcctgttc tttctcatcc gtatccgcct
    22681 gtttcgcgaa ctgttgcaca agtcggcgca ctccccaaaa gaattgctcg ccgttaccgc
    22741 cattttctcg ctgttcgccc tgttcagcac ctggtccggc gttcccgtag aaggctcgct
    22801 ggtgaacgta cgtattatcg cggtgatgtc cggcgggatt ctgtttggcc cgtgggtagg
    22861 catcattacc ggcgtgattg cgggtattca ccggtattta attgatatcg gcggcgtgac
    22921 ggcgatcccc tgctttatca ccagcattct ggcgggttgt atatcaggct ggataaacct
    22981 gaaaatcccc aaagcacagc gctggcgctg gatatcgcca acaaaacgct accgctgttt
    23041 cgccatgtca atagcgagtc attacgcaag gtctgcgaaa ttatccgcga tgacattcac
    23101 gccgatgcgg tggcgattac taataccgat catgtgctgg cctatgttgg cgtgggtgaa
    23161 cataactatc agaatggcga tgacttcatt agcccgacta cccgtcaggc gatgaattac
    23221 ggaaaaatca tcattaaaaa caatgatgaa gcccaccgca caccagagat tcattccatg
    23281 ctggtgatcc cattgtggga gaaaggggtc gtgaccggaa cgctgaaaat ttactactgc
    23341 cacgcgcatc agatcacctc gtcattacag gaaatggcgg tcggtctgtc gcaaatcatc
    23401 tccacgcaac tggaggtttc acgcgccgag cagctacgtg aaatggcaaa taaggcagag
    23461 attcgcgccc tgcaaagcaa aattaatccc cattttctgt ttaacgctct gaacgctatt
    23521 tcatcgtcaa tccgtctgaa tccggatacc gctcgccagt tgatctttaa tctgtcgcgt
    23581 tatctgcgct ataacattga attaaaagac gatgagcaaa tcgatatcaa aaaagagctg
    23641 tatcaaatta aagactatat tgccattgag caggcccgct ttggcgacaa gctgacggtt
    23701 atctatgata ttgatgaaga ggtgaattgc tgcattccca gcctgctgat ccagccgttg
    23761 gtggagaacg ccattgtcca cggtattcag ccttgcaaag gtaaaggcgt tgtcaccatc
    23821 agcgttgcag agtgcggaaa tcgggtacgc attgcggtgc gagataccgg gcacggcatc
    23881 gttccaaagg tgattgagcg ggtcgaagcg aatgaaatgc cgggcaataa aattggcctg
    23941 ctgaatgtcc atcatcgcgt gaagttattg tatggcgagg ggctgcatat ccgccgcctg
    24001 gagccgggga cggaaattgc gttttacatt cctaaccaac gcaccccagt cgcctcacag
    24061 gctacgttat tgctttgagc cggagtgata ttgtgaaagt catcattgtt gaagacgaat
    24121 tcctggcaca acaggaactg agctggctaa ttaaagagca cagccagatg gagattgtcg
    24181 gcacctttga cgacggtctg gacgtgttga agtttttgca gcataaccgc gtcgacgcca
    24241 tttttctgga tatcaatatt ccgtcgctgg atggcgtgtt gctggcgcaa aacatcagcc
    24301 agttcgccca taaaccgttt attgtgttca tcaccgcgtg gaaagaacat gcggtagaag
    24361 cgtttgaact ggaggcgttt gactacattc tcaaaccgta tcaggagtca cgtattaccg
    24421 ggatgctgca aaaactggaa gcggcctggc aacaacagca gaccagcagt acgcctgccg
    24481 cgacggtaac gcgtgagaat gacaccatta atctggtgaa agatgagcga ataatcgtca
    24541 cgccaattaa cgatatctat tacgccgaag cgcacgagaa aatgaccttt gtctatacgc
    24601 ggcgtgaatc ctacgtaatg ccgatgaaca ttaccgaatt ttgcagcaaa ctgccgccgt
    24661 cgcatttttt ccgctgccat cgctcatttt gtgtcaatct gaacaaaata cgcgaaatcg
    24721 aaccgtggtt taataacacc tacattctgc gactgaaaga tctggatttt gaagtgccgg
    24781 tcagccgcag caaagtgaaa gaatttcgcc agttaatgca tctttaatcg ggaactttca
    24841 tgaaagcacc aggcttgccc gccgatcaac aatttttcgc cgatctgttc agcggcctag
    24901 tgcttaaccc gcaactactg gggcgcgtct ggtttgccag ccagcctgcc tcgttaccgg
    24961 tgggcagttt atgtattgat tttccccgtc tggatatcgt gctgcgcggc gaatacggca
    25021 acctgctgga agcaaagcag cagcgaatgg tggaaggaga aatgctgttt attccggcac
    25081 gcgcggctaa tttaccgatc aacaacaaac cggtgatgct gttaagcctg gtgttcgcac
    25141 cgacctggct tgggttatcg ttttacgata gccgcaccac gtcgttgttg catcctgctc
    25201 gccagaccca gcttcccagc ctgcaacgcg gtgaaggtga agcgatgctt accgccctca
    25261 cccatcttag ccgttcgccg ctggagcaga atatcattca gccactggtg ttaagtttgc
    25321 tgcatctttg ccgcaacgtg gtgaatatgc cgccgggtaa ttcacaaccg cgcggcgatt
    25381 ttctctatca cagcatttgt aactgggttt aggataatta tgcccagccg ctcacccgcg
    25441 agagcgtggc gcagtttttt aatatcacgc ccaatcatct gtcaaaactg tttgctcagc
    25501 atggaacgat gcgttttatc gagtatgtgc gttgggtgcg aatggcgaag gcgaggatga
    25561 ttttgcagaa atatcatctg tcaattcatg aagtggcaca gcgttgcggt tttccggata
    25621 gcgactattt ttgtcgcgtt ttccggcgtc agtttagtct gacgccggga gagtacagcg
    25681 cccgttttca gggctaacgt cagaaggtta attctgtttc cagcagcgtc aggatacttt
    25741 gtgcatcttg cgcggcaaac aacgactggc ggaagttttt attcaccagt ttacgcgcca
    25801 gctgcgagaa gactttcaca tgattcatcc cttcgttagc acccagcgtc agcatgatca
    25861 ccagttcgac ttcgcccatt tctgactgcc agtcaaccgg tttcgccagc cgggcaatgc
    25921 tgatactgga atgacggatc cactgagatt tggtgtgcgg gatcgctacg ccaaaaccaa
    25981 cgccggtggt aacaatctct tcccgctgcc agacatcttc ttccagctca aacggatgtt
    26041 cagtgcgccc gttaacgccg aggttgccgc acaggaactg gatcgcctgc tctttattgc
    26101 taaaatcctg atcaacaaag atattctcca gcgccagcag tgggcgaacg tcttcttccg
    26161 gcgtaaaggc ggtgagtaac gcttcaattt cctgcgcact gcggcattca catgcctgac
    26221 gcgccagttc ccgacacgcc tcgctatcca gttgacgaag ctggcttttc accgccggaa
    26281 tacgcgggct actcatactc agctcatcca ggcccagccc aagcagtagc ggcagataac
    26341 ggctttcacc gcccagttca ccgcaaatgc ctacccattt gccccgctga tgcgcagtgg
    26401 taactatttg ctgcaacatg cgcaggaacg atggcgtaat cgggttatat agcggcgata
    26461 cgcgcgggtt attacgatcg accgcataca gatactgggt catatcgttg gagccgatac
    26521 tgaagaaatc gacctcatcg cagaagtggt cgatgatgta gcacaccgac ggcacttcca
    26581 ccatgatccc aagcgtaatc gtctctgcat gacgcaggcc atcgcgctta agctcaacga
    26641 tcgctttttg gatctcgcct ttcacccata agatctgatc gaggctgtga accatcggga
    26701 tcatcaactg ggcgttgccg aaactggcgg cgcgcaaaat ggcccgcagt tgagtgcgga
    26761 acaggccagc aaattccggg taaatacgta ccgcgcgata gccgaggaac gggttctctt
    26821 cctggggaat attcagataa ggaatgcttt tatcgccgcc gatgtccatc gtgcggaaga
    26881 taatcggctt gtcgcccgcc gccagtagca cctgctggta ggcttcaaat tgctcctgct
    26941 cgtccggcgc gctgtcgcga tccatataga gcatttcggt acggaacagc ccgacacctt
    27001 ccgcgccgtt ggcaaacgcg cctggcgctt ccagagcggt gccgatattc gccgcaatat
    27061 caatacgctt gttatcacgg gaataggcca gctgcgcggc agcctgcgcc tgttgttttt
    27121 ggcgtttatc cgccagcgtc tgcgcgacct gataataacc gctcaccgcg tcattcgggt
    27181 taatcgccag cacgccgcac tgggcgtcaa gcactgcagg ttgcccggca taacgggcaa
    27241 tcgcatccag cggcaagcca ctcagtaccg ggatcgccga ggcacgggcc agaatcagtg
    27301 tatgcgaggt gcgcccggtt ttctccagaa tcatacccgc aagatttttc aaatcaaggc
    27361 tcaaaaactg gcttggggtt aaatcttcag ccaccagaat ggtcggtttt tccagcacca
    27421 gattgttgcg cggcttcagt tccggccagg tgatatgcag caactgttcg ctgatatcgc
    27481 gaatgtcgct gacccgctcg cgcagataat cgctggcaga agcagatagt ttggcgcaaa
    27541 cctgctccat attgctgatg atcgccgccc ccagtccctg atgctgttct gtcatcaggc
    27601 gacggatatt gcctgcaaat tcatcatcct gaatcagcga caaatgagcg ctgaggatag
    27661 ttttgctttc gccgtcacgc tcacgcaatt gctgattcag ttgctcggca agcgttgcca
    27721 ggctgtgctc cagccgggtg gaatcttgcg cactggcggg gattgcccga taactgtcga
    27781 ggctgtcgct ttgtaacagg gtcagcgtac ccacgccgac gccgcttgcc agcacattgc
    27841 cgtacagtaa atccgggtta aggcggctta atgaacgcgg cagcggatgc gccgtcagtt
    27901 ctgcctgcgt cggctgaacg ctgtcgctgt cgataaagcg caccaggatg tactcttcca
    27961 gcacgcgccg cgcctgctct tcatcgctgc cgctaatgtt caggctgcaa ctgtcattaa
    28021 ataaggtgcc agttccaatc aacgccagcg agcttttagc atcagccttt gcgttctggc
    28081 gatggttgat aaaggtgatt tcgctttgcc actggctgca ctgttcttta agttcccacg
    28141 ccggacgagc atgtagaccg ttaggcaaag gacagagaaa ttgaatcgtt aacataaccg
    28201 ctccgggtta atcaggagat catctgaaat ccgtcagttg aacaaccgtc tcacgcgtaa
    28261 gacgttgaat aagggcagat aaaagttgct ccatctgcaa aatgtcgcgg caatcggcaa
    28321 tcgatgccgc gcaatgtccg tggcgggtgg caggccccat taccaatgtg ggtacgccag
    28381 taccggttaa gtgcaccgct ccaccgtccg tgccgccgtt actgaacata tccgcctgca
    28441 acggaacgcc aatttctgcc gccacggttt caatccacgc ggtaagtttt ggcggcgcaa
    28501 tcagcgattt atcgcttaac accagcatcg gcccgttacc aatctgacga tggttagccg
    28561 cgccataatc aaagtttttc gcccagcagg cggtatcaag cacaatggcg acgtccggcg
    28621 acaccgcgcg ggtagcagtt tgcccgccgc gtaatcccac ctcttcgctg gaacttgcca
    28681 ccagccacac ttccgcaggc agttcggcgt cgtgcagctc acgtagtaac gtcaccagca
    28741 gatagcaact gaggcgatca tcaaaggctt tgcccatcac ccgctggtga gggaggacct
    28801 gaaaagtggt atcgaaggtg acgcgatcgc ctggacgaat tcccgcctgc atcacttcgt
    28861 cataggtgcg cgcgccaatg tccacgcgca tggcgctaac gtcattcccc tgccggtcgc
    28921 cgtcaagcag gcctggaatt ttgcactctt cacgggtggt gatgcgcacc ggctgcagct
    28981 ggcgggcagc catgcgcacg ttgccaaccg gcagcacgtc aatagcgcct tcgcgggaga
    29041 tgctgcgcac cataaatccc acttcgtcca tatgggcgca gatcatcacc ttcggacctg
    29101 tcgattcatt gaggcggatc agcaccgatc ccagaccatc aaatcgcact tctttttgca
    29161 ggcgagccgc ttcttccagc aggatctgcc gcacttcctg ttccgaggag gcgatcgcat
    29221 ctgcctcgct caacgctttt aatagcgata aatccattat gcctctcccg tgagcaacac
    29281 tgttttcggc atggcgtaga gcacttctgc gccttgcggg gtaaccagca caacatcttc
    29341 gatgcgcacg cccccttgcc ctagcaaata aatccccggc tccacggtca gcaacatgcc
    29401 tggctgtagc gtcgtggtgt cccgcggtga aaaacgcgga tcttcatgga cttcaatgcc
    29461 gatagcgtga ccagtgttat gaccgaaata gtcgccaaat ccagcctcgg taatgacccg
    29521 gcgcgcggcg tcgtcaatct gctggcagcg cacgccgggg cgaattgcag agattgccgc
    29581 gagctgtgcc tgcaggacaa tctgatagac gtcaaacagc gggtgagatt cggcgctcac
    29641 cccttcgcca ttcaccagca aggtgcgcgt catatcagca gtagccctga tacagcgcac
    29701 caaaatcgag agtgacaaac tcgcccgctg caacaatctt gtcgctggct ttgccgtgcg
    29761 gcagcgcccc gcgccagccg ctggcgacaa tggtgtcaaa agaggctttt tctgcgccct
    29821 gctggcgcat aaaccactcc agttcagcgg ctatctcgcg ctcgctcatc cccgcctgaa
    29881 taaagcggcg aatatgctcc gctccgcgat cggcaatccc acaggcgagg cggattatct
    29941 ccacctcctc tggcgttttg atttgccgca gcacatccgg cgtggcgcta accagtttcg
    30001 cattgagttc agacttccag cggtgcgcgg tttcccaact cacctgctgg ccttcaaaac
    30061 cgagcgtttg caactgctca tcggcaatga tttgattgac gatagtggta agcgtgttcg
    30121 ttgcgtcaag caaatgcagc tggtagcctt gcgcacgggc ttccacctca acgaaatagc
    30181 gcgaatctac cagaatgtgc gcactttcac ggctaataac cacatagcct gatccggtgg
    30241 agatccccag atgcggctgt ttgttctgcc gtgaggagag aagcaccgca tccagttgtt
    30301 gcaccttaag ccagttgcgc agcgaagcga gtaatgtcat ctggattttc cttcttacag
    30361 gctatcgatt aacaatttgc ctttgcggaa catcatcaga cgcaggaaca ccaccatcag
    30421 cgcagtaatg attgccccca gcgcgatccc cgccatataa acgcccaagt tagtgaccag
    30481 cggccatgcc cagatagcgg attccgggaa ccattgcact gcgcccagcc agacagctgc
    30541 ggttgagccg acaatcgcgc cgaccatata tgacggaata gcggtgatgg ggctttccag
    30601 cgcaaacgga atcgcaccct cactaatccc cataaaggcc aggaacatcg cggtcttacc
    30661 ctgcggataa agttgagcgt tgaacaggcg tttgccggtt aaacgacggt caataatggt
    30721 cgccagaccc agaccaatcg gcggaataac gatagcgata gaacgagcgg tgaccggtaa
    30781 tacgtggtca gtggtaaagc tgaaggcaac gaaacctgcg gctttgttga tcgggccacc
    30841 aaggtcgatt gctgtcgccg cggcgatccc cattgcgtac atcagcgcgc ccttctcacc
    30901 tgcggcagtc agcacagtac ggataccgcc gttgatccag ccaccaaagg gagtgatgac
    30961 gtagtacatc gccagcatga caaaaatggc ggaaagaatc ggtagcaaaa atgtggtttt
    31021 gaacgctaac aggaaatccg gtaactggat tttctggttc atccatttca caaggtaacc
    31081 ggcaacgata gaaataatca gcgcaccaat gaaggtggac ggcaccggcg aagaggtcgc
    31141 ccattgcata gtgctgggat cgaagttcag cagttgcgtc ggctgggtag acatcaaccc
    31201 gccgataaaa ccagccggga acgccagttt gccaccgata gagttcgcca caaatgcagc
    31261 aaacatcgga atggcaaaac caaacaacac gccgccgaag gactgcgaca gccaggcaaa
    31321 tttaagtaag gagaggtcga aaccggagaa tttcccgcta ttaagagcat ccatgatgcc
    31381 gatctcagca ggaattttaa gccagctata agcaatcagc tggctgaaag cgaggatcac
    31441 accgcccata attaaggtcg gcaccatgcg tgaaatccct gacatcacat gctgcgggag
    31501 ttcaccccag aagctgcttt tagaggcctg cggattctta actgccgctg ccgcaccgga
    31561 tgcgccaggc acaacggttg cactgcgttt tttaatggcc ataacaatta tccctgtcga
    31621 atattactgt tgttcagagg caatcatctc ttcgatttct ttgatgatgc ccgcagcgtt
    31681 tttaattgcg tcctgcaaag tgatttcata aacgtcgcgt gattcgaaac gttcgttatc
    31741 ttccggggta actgccacgg aatggatgat gatggtcgct tcggcgatat cctgcgccgt
    31801 caggcgattc tggatgccgt ccgcgccctg ggtttcaatt ttcacttcat aaccggcttc
    31861 taccgccgct tcttccagca cctgagcggc cataaaagtg tgagccaggc ccattgggca
    31921 ggcacataag gcaatcagtt tcttactcat cgttaattcc tcgttcagtg atagttgact
    31981 agtgaaatta tgaacgtgca aaacaaatcg ccgttaccag acaaataatg catttactgg
    32041 agaattaatc ccgctgtacg gaagtgtgac gcaggtcggc cttgtgaggg gacacaaaga
    32101 aaaattgaat taactgtatg atttaaaaga ttatcgggag agttacctcc cgatataaca
    32161 ggaaggattt acagaatgtg acctaaggtc tggcgtaaat gtgcaccgga accgagaaga
    32221 cccggatagt catggacgat gagatacacc ggaatatcat ggacatattc tttaaagcgc
    32281 cctttatctt caaatgcggc acggaaaccg gaggctttga agaactcaag gaagcgcggc
    32341 acgataccgc ccgcaataaa cacgccgcca aatgtcccga gattgagcgc cagattgccg
    32401 ccaaaacggc ccataatgac gcaaaacagc gacaatgcgc ggcggcaatc ggtgcagctg
    32461 tcagccagcg cgcgttcggt aatatctttt ggcttgagat tttctggcag gcggttgtca
    32521 gctttcacaa ttgcgcgata caaattcacc agcccagggc cagaaagcac gcgctccgcc
    32581 gaaacatgac caatttccgc acgcaatatt tcgaggataa tggcctcttc ttcactattc
    32641 ggcgcaaaat caacgtgacc gccttcgccc ggcaagctta cccaacgctt atcgacatgg
    32701 accagatgcg caaccccaag ccccgttccg gcaccgtaaa ccgcaatagg cttaccttcg
    32761 accggttctg cgccaccaaa ctgaatcaga tgctcttttt tcagcatcgg gatcgccatc
    32821 gatacagcgg taaaatcgtt aataatttcc agatggctaa aaccgagatt ctttttcatt
    32881 tcggcaattg agaacgccca ggtatggttg gtcattgcca cccagtcacc ggtaattggg
    32941 caagcgatgg caatacagcc gtctttcacc tcgaccttat gttcttcaag ataaacgcga
    33001 atgaccgctt cgaggctggg gtaatcaagc cctgaatagg tcttagcctg cgagatttca
    33061 ccactggcaa tatcacacag agcaagacgt gcgttggtgc cgcccacatc accgactaat
    33121 gcatactttg tcattcttca actgctccgc taaagtcaaa ataattcttt ctcacactgt
    33181 aaatacctgg gggcataaca acaacgccgg aaaggcaggc tccctgtaaa tatcgatctg
    33241 ggtcacacaa ttactttatc gtttcagcac caattgcagc gatgcctttt tgcaagctgg
    33301 gcaaactaag tatctgaccc cgcataagga atagaacatg ctccatccgc gagccagaac
    33361 catgttgtta ttatcgctcc ccgccgtggc aattgggatt gcgtccagtc ttattctgat
    33421 tgtggtgatg aaaatcgcct cggtattaca gaatttgctc tggcaacgac tgccgggaac
    33481 tctggggata gcccaggatt cacccctctg gatcatcggt gtattaacgc taacgggtat
    33541 tgcggtgggg ttggttatcc gtttcagcca gggtcatgcc ggaccagacc ccgcctgtga
    33601 accgctgatc ggcgcaccgg ttccgccctc tgcgctacct agacttatcg tagcattaat
    33661 tctcggtctt gctggcggcg tcagcctggg gccggaacat ccgatcatga ccgtcaatat
    33721 cgcccttgcc gtcgccattg gcgctcgtct gttaccgcgc gtcaaccgaa tggagtggac
    33781 tattttagcc tctgccggaa ccatcggcgc gctgtttggt acacctgttg cggcggcgtt
    33841 gatattttcg caaaccttaa atggcagtag tgaagttccg ctatgggatc gtctttttgc
    33901 gccgttaatg gcggcagcag ctggtgcact tactaccgga ttatttttcc atccccattt
    33961 ttcactgccc attgcgcatt acggacagat ggaaatgacc gatattctca gcggtgcaat
    34021 tgtcgcggcg attgccatcg cagcagggat ggtcgccgta tggtgcttac cacggttgca
    34081 cgcgatgatg aatcaaatga aaaatccggt gctcgtgctg ggtattggcg gatttattct
    34141 cggaattctg ggggttattg gtggaccagt ttcgctgttt aaagggctgg atgagatgca
    34201 gcagatggtg gcaaatcagg ctttcagcac cagcgattac tttttgctgg cggtaattaa
    34261 acttgccgcc ctggtcgttg ctgccgccag tggctttcgc ggtgggcgaa tcttcccggc
    34321 agtgtttgtc ggcgtggcat tagggttgat gctgcatgag cacgttcccg ccgttccagc
    34381 ggcaataacc gtttcctgcg ctattctcgg catcgtgctg gtggtaacac gcgatggctg
    34441 gttaagtctt tttatggcgg cagtcgttgt acccaatacc acattgctac cgctgctctg
    34501 tatcgtcatg cttccggcat ggctgttatt agcaggtaag ccgatgatga tggtcaatcg
    34561 tccgaagcaa cagccacccc acgataatgt ttagcaaaaa atgctctcct ttatgattta
    34621 agagttatgg cgatgatacg gggctttacc tccccgtaat attgcgttaa caggccgctg
    34681 acggcttcga tcgtgaagga gaataacaat gttcaggtca ctgtttctgg cggccgccct
    34741 gatggcattt accccgcttg cagcaaatgc aggtgaaatc accctactgc catcgatcaa
    34801 attacaaatt ggcgatcgcg atcattacgg taattactgg gacggtggtc actggcgaga
    34861 tcgtgattac tggcatcgca attatgaatg gcgcaaaaac cgctggtggc gtcatgataa
    34921 tggctatcat cgtggctggg ataagcgtaa agcatatgag cgtggctatc gtgaaggctg
    34981 gcgcgatcgt gacgatcatc gcggaaaagg ccgcggacat gggcaccgcc attaaacgcg
    35041 tcctccaatg gagcacaatg cctgatgcga cgctagcgcg tcttatcagg cctacaaacc
    35101 ggctctttaa cgcgtagggc ggataaggcg tttacgccgc atccgccaat agtgccagat
    35161 gcgacgctca ttcaactaca atcccagcgc cgtccccacc aacaaccaga tattcagcgc
    35221 cacgaccagc actacaatca cccagcctgt ctgttttacg cgtttgctgt tcaccagatc
    35281 gcccatcaac ttgctgtcac tggtgaaaat cagcagtgga accagcgcca gggcgatacc
    35341 aaaacttaac agcacctgac tcataaccag aatccgtgtc ggatctaatc ccatcagaat
    35401 gacaataaat gacggcaaca tggtgactgt acgacgcacc cacagcggga tatggaagcg
    35461 aatgaatccc tgcatcacca cctgccccgc cagtgtcccc accaccgttg aggacagtcc
    35521 ggcagcaacc agacttaacc caaagaccgt tgccgcagca tggcttaaca gcggttgcag
    35581 cgtcagataa gcctcatcaa gatcggcaac accagtatga ccagaaaagt ggaacgccgc
    35641 cgcagctgta gccatcatca ccagattgac aaaaccggca atcgtcatgg cgatagccac
    35701 atcccatttg gtggcggaat aacgttgttg acgcgaaccg ccatgtaaat gctgagtgag
    35761 cgaagagtgc aaataaatca catgcggcat aatcgtcgcc cctaacacgc ctgctgccag
    35821 gaagaccgcc tccgaagtag gtaaactcgg gatcaccatt cctttaccca gctgcgccag
    35881 gttaggctgg gagaaaatca actcgacaat gtaagccgcg gcaacaaaca acagtaaccc
    35941 gccaatcact ttctccagcg gtttttgccc gcgacgttgc agcattaaaa tcaggaaagt
    36001 cgcgatcccc gtcagcaccg cgccctgcaa caacgaaaca ccaagaatga gtttaaaacc
    36061 gatcgccgca ccaataaatt ccgccaggtc ggttgccatc gcaataattt ctgcctgaac
    36121 ccaatagaac cacactacgg gacgcggata gtgatcacga atctgctccg ccagattttt
    36181 accggtggca atccctagtt tggcagagag gatctgaatc agcatcgcca tcaggttggc
    36241 ccaaacgaca acccacagta gctgatagcc gaagctagca cccgcctgaa tattggtcgc
    36301 aaagttaccg ggatcgatat aaccaatcgc cgcaatgaac gcaggtccca ttaatgcgag
    36361 cctcatctta cgcgccgccc gtccgctgct actctcaacg cgatagttcg tcatcttgtg
    36421 cctctaaaac atagcctttg ctatgtttca tgctatgcta aacgagaatg attatcaaat
    36481 tcatttaaat ggattgtggt gatttctctg atagaccaag attatgatta cgaaaagatt
    36541 gatgacttga atgttatggt tatgtttaat gttagcacat ttacataact ttcagcttcc
    36601 atacacaaca tagcagaaat gtatgacaga tcactatttt tgaagcctgt cacaggacgt
    36661 cattatagtg tgtgtcagat ctcgttttcc ttaaccatgt tacatagaat gtgcacggaa
    36721 atttaacctg cctcatattt ggagcaaata tggaccgcgt ccttcatttt gtactggcac
    36781 ttgccgttgt tgtgattctc gcactgctgg taagcagcga ccgcaaaaaa attcgtatcc
    36841 gttatgttat tcaactgctt gttatcgaag tgttactggc gtggttcttc ctgaactccg
    36901 acgttggttt aggcttcgtg aaaggcttct ccgaaatgtt cgaaaaactg ctcggatttg
    36961 ccaacgaagg gactaacttc gtctttggta gcatgaatga tcaaggcctg gcattcttct
    37021 tcctgaaagt gctgtgccca atcgtcttta tctctgcact gatcggtatt ctccagcaca
    37081 ttcgcgtgtt gccggtgatt atccgcgcaa ttggtttcct gctctccaaa gtcaacggta
    37141 tgggcaaact ggaatccttt aacgccgtca gctccctgat tctgggtcag tcagaaaact
    37201 ttattgccta taaagatatc ctcggcaaaa tctcccgtaa tcgtatgtac accatggctg
    37261 ccacggcaat gtccaccgtg tcgatgtcca tcgttggtgc atacatgacc atgctggaac
    37321 cgaaatacgt cgttgctgcg ctggtactga acatgttcag cacctttatc gtgctgtcgc
    37381 tgatcaatcc ttaccgtgtt gatgccagtg aagaaaacat ccagatgtcc aacctgcacg
    37441 aaggtcagag cttcttcgaa atgctgggtg aatacattct ggcaggtttc aaagttgcca
    37501 ttatcgttgc cgcgatgctg atcggcttta tcgccctgat cgccgcgctg aacgcactgt
    37561 ttgccaccgt tactggctgg tttggctaca gcatctcctt ccagggcatc ctgggctaca
    37621 tcttctatcc gattgcatgg gtgatgggtg ttccttccag tgaagcactg caagtgggca
    37681 gtatcatggc gaccaaactg gtttccaacg agttcgttgc gatgatggat ctgcagaaaa
    37741 ttgcttccac gctctctccg cgtgctgaag gcatcatctc tgtgttccta gtttccttcg
    37801 ctaacttctc ttcaatcggg attatcgcag gtgcagttaa aggcctgaat gaagagcaag
    37861 gtaacgtggt ttctcgcttc ggtctgaaac tggtttacgg ctctaccctg gtgagtgtgc
    37921 tgtctgcgtc aatcgcagca ctggtgctgt aaaaccatac ataaaaaagc cggggatcat
    37981 tccccggctt ttttacgcct gttaatcagc taatggctgc gggcgaccaa tcaaataccc
    38041 ttgcagatat tgcaccccga gcttatgcaa tagcgcctgc tgctgttgcg tctcgacaaa
    38101 ctccgcgacc acactcaatg acttcgcttt cgccagatcg gtaattgatc tcacaatcat
    38161 cgcatccagc gtattcgtga caatatcttt cacaaagacg ccatcaattt tgatgatatc
    38221 agcctgcaaa cgctttaacc gttcgtagtt ggcatatccg gtgccaaagt catcaatcgc
    38281 aatccggaaa ccaaacttat gcagctgctc gatgttgtac atgctgcttt ctgcgttaga
    38341 aaacgcctgc tcctcggtga tctcaagaat gaccgcctgc ggggagatgt gataacgctt
    38401 aaacagacga ataatccgcc cggcaatatt cttttgcagc agcgtgagcg gcattaaatt
    38461 gactgaaaag cgcggtcctt ttttgtcgca agggtgtgtt gccagccact tcaacaagga
    38521 ttccagcact tgcaaatcaa aacgcgcgct aaggttaaac tgagcaataa ggggcagaaa
    38581 cttatccggg gtcataatgc cgccgtcata tttcagtcgc gcgaggatct catcataacc
    38641 ttcaccctct ttgttgcgaa ttggctgggc gtagagcagc aaatcaccct gatctaacgc
    38701 cgtgcgaatg gtattcagca atagcacctg tttagtggtc tgcccggaaa ccatctcctc
    38761 tctgctatcc agcgccagca catgatgatg tgcgcaggat tgctccgcca gccagcttaa
    38821 ctgccccaac aagggttgca gggtttcctg atttccatca aaacgccccc aggcagcacc
    38881 atagcccata tccagcccgg tattgttcca gtgaatttgc cgactattca ggatgttaac
    38941 catatgctgg agtcgccctt ccgtttccgg cccgctcagc actaacagca gttcactacc
    39001 cggcaattga tacaactttt cgttttcctg catcaacggc agcagcgtac ggcaaattga
    39061 gcggatacag tgaacgcgca tcattaagcc gtaatgacga ctcataaact caagattatc
    39121 aatgcgcagg cagcaaaaac tcttgcccgc ctcttgctcc ggcgcttgtt ccaacgcacg
    39181 aaagttgggt aggagcgtta acggatctgt tagcgcctgc aaatgccagc ggcgattaag
    39241 ccattcactg cgatgataaa tgcgcaccat atagagcagg caaacgctaa aggaaatcag
    39301 caccgcgaga ataaatgcca gcgaatattc ggtttccacc ccttgcaaaa agttctgatt
    39361 gtaattcaga aggcaaagcg ttgaaacagc ccaggtgaga tttaaaaacg gatagcgaag
    39421 attaccgacc ccgagggtaa agatgatgaa gaaaacgggt accaggtaac cggcaataaa
    39481 gtcgttttca taaggtgtgc acaacagaag tagcagcacg cttaacgctg ccagccagct
    39541 taaggtaaat gcgagtgaga tagtagaaaa gcatgttgta aatcagcact gcggtgaaaa
    39601 ggcttagtaa atcaacgacc gtgaaaatgg catccgcatc gccgaaaaag gtggatatct
    39661 ttagcgggaa atcaaagaag ctccccacaa gatacatgct gcatttgatg ccgatcggcg
    39721 tcaccaaacc aagccagaac agacgttgcc agacatatcg gctggtcaat ccataacgcc
    39781 aacgcgtccc cagctgccag cgtaatattg cgcaggcaca caacacgaca aacgtctggc
    39841 tgccgagcat gaccgcagtc tgtaaaaaag agagatgaaa gttccacaga ttggtgcaaa
    39901 acatcccgca taaaacggga acgacaccgc gccagccaaa gataaacaag attgacagca
    39961 ttacgcacag tggcatccac gccagaaaga tataactgga atggataatg gcaagtggcg
    40021 aaataaaacg ggatagctga ataagtacca cggtgagcgt aaacgctagt gtgaatatct
    40081 tgatattttt tatcaggtta tgctccacaa acatgagtac actactctca ctgatcgggg
    40141 ttttcaggca cacaaataat aagtttattc gcatcgcgaa gcaaacaggg gccagcctgt
    40201 tgccgatttt tcatccaatg atgctttcac caggcggcgc taacccggac gtagactgta
    40261 ttacaaaagc ggcaaaaagc agagacaaaa aacccccgct ttgcagcgag ggttggaaat
    40321 ttggtggagc taagcgggat cgaaccgctg acctcttgca tgccatgcaa gcgctctccc
    40381 agctgagcta tagccccgtc acgtaaagct tgtcgagttg acgggcggca tcatatgaat
    40441 tccgcccgaa tgtgtcaacg gcaaattgca acgtgtagtt tcaatcgctg aaaaatcagg
    40501 caaatgaaca attttggaaa accgctcgca ttcagtagtt attcatgtca cggtttcctg
    40561 taaagtggtg ttataaaatg aactactaat agacccacat acattcaggg aattgttatg
    40621 ttcaaggaga ggatgacacc agatgaactt gccagactga ccggctatag ccgccagacc
    40681 attaataaat gggtacgcaa ggaaggctgg acaacgtcac caaaacctgg cgtccagggt
    40741 ggcaaagccc gactggttca cgtcaatgaa caggttcgtg aatatattcg caatgctgaa
    40801 cgtccagaag gtcagggaga agcgcctgcc ctttctggtg atgcgccgct tgaagtattg
    40861 ctggtgacac tggcgaaaga gatgacgcca gttgaacaaa aacagtttac atccttgctt
    40921 ctgcgggaag ggattatcgg attgttacaa cgcttaggga ttcgcgatag caaataatat
    40981 gaaaagatta cgcaataaaa tgaccaccga agaactggct gaatgcctcg gtgtggctaa
    41041 acaaactgtt aaccgttgga ccagagaaaa aggctggaaa acggaaaaat ttcccggcgt
    41101 gaaaggcggt cgtgccagac ttattctggt cgatacgcaa gtttgcgagt ttattcagaa
    41161 cacgccagcc ttccataaca caccaatgtt gatggaagct gaagagagaa ttgctgaata
    41221 cgctcctgga gcacgcgccc cggcgtatcg acaaatcatc aacgcgatag ataatatgac
    41281 agacattgag caggaaaaag tcgcgcaatt tttgtcacgt gaaggaattc gcaatttcct
    41341 cgcccgtctc gatattgacg aatcagcata aataaaaaac ggcaggatat tatctcctgc
    41401 cgtttatctt tttacacgct aattactgct gattttcgcg ttcagcaata aaatccagcg
    41461 ctttgttgat acgctcgata ctgcgggtct taccaattgc gtgaacggta acatccagtg
    41521 ctggagactg ccccgcaccg gttacggcga cacgcagcgg cataccaact ttacccatac
    41581 ccacttccag ctcatccgcc gtcgcctgaa tagcgtgatg aacgttttca gcggtccagt
    41641 cagtaatcgc ggccagtttg tcacgaacca cttccagcgg ctgacgcgct accggacgca
    41701 gatgtttttt cgccgcatcg gcatcgaact cagcaaaatc ttcgtagaaa taacggcagc
    41761 tctgtgccat ctctttcagc gtcttgcagc gttcgcccag cagtttcacc agatcagcca
    41821 gctgcgggcc gttacgggta tcgatatttt cctgctcaat gtgccactgt aagtgagtag
    41881 caacatactc cggcggcagc gcgttaatgt agtgatggtt cagccacagc agcttgtcgg
    41941 tgttgaacgc actggcagat ttgctgacgg cattcaaagt gaagtatttg atcatctctt
    42001 cacgagtgaa gatttcctga tcgccgtggg accagcccag acgcaccaga tagttcagca
    42061 gtgcttctgg caaataaccg tcatcacgat actgcattac gctgactgcc ccgtgacgtt
    42121 tggacagttt tttaccgtca tcgccattga tcatagaaac gtgcgcgtaa accggcaccg
    42181 gcgctttcag ggccttaaga atgttgatct ggcgcggcgt gttgttgata tggtcttcgc
    42241 cacggataac gtgggtgatt tccatatccc agtcatcgac aaccacacag aagttatagg
    42301 ttggggaacc atcggtacgg cggataataa gatcgtccag ttcctggttg ctgaactcga
    42361 tcggaccacg gatctgatcg tcaaaaacaa cagaaccttc ctgcgggtta gcaaaacgta
    42421 caacacacgg ttcatcatca gcatgatgct catggctgtg gcggcagcga ccgtcataac
    42481 gcggcttctc acctttcgcc atttgctctt cgcgcagcgc ttccaggcgc tctttagagc
    42541 aatagcattt ataagcagtg ccctcttcca gcatctgatc gatcaccgcg ttgtagcgat
    42601 caaaacgttt ggtctggtag tacggacctt catcccactc caggctcagc cagttcatgc
    42661 catccataat ggcttcgata gcttccggcg tggaacgctc aagatcggtg tcttcaatac
    42721 gcagcacgaa ctcaccgccg tggttacgtg caaaaagcca ggagtaaaga gcagtacgcg
    42781 cgccgccaac gtgcagatag cctgttgggc ttggcgcgaa gcgagttttg attttcatga
    42841 aatggcctta cgtttagaaa gatgccgaca accggcaaat cctggaaaaa ttgagtgggc
    42901 gatattctat cactcccgcc tgattcctca atgtagttcg ggtttctacc ttacgcatta
    42961 ttgttttcgt tgacaaattg cgcaacacgg tctgttttgc gatcatcttg cttaaattta
    43021 cgacgaacga acaatttctt tagaaaatgc gttgactcat tttgaactct ccctataatg
    43081 cgactccaca cagcgggggt gattagctca gctgggagag cacctccctt acaaggaggg
    43141 ggtcggcggt tcgatcccgt catcacccac caactacttt atgtagtctc cgccgtgtag
    43201 caagaaattg agaagtgggt gattagctca gctgggagag cacctccctt acaaggaggg
    43261 ggtcggcggt tcgatcccgt catcacccac cacttcgggt cgttagctca gttggtagag
    43321 cagttgactt ttaatcaatt ggtcgcaggt tcgaatcctg cacgacccac caatgtaaaa
    43381 aagcgcccta aaggcgcttt tttgctatct gcgatactca aagattcgaa cctgcagcag
    43441 gtttgagttg agcgcagcga aacaacggag ccgctcgcgg cgacggcccg aagggcgagc
    43501 gaagcgagtc atcctgcacg acccaccaat gcaaaaaagc gccctaaagg cgcttttttg
    43561 ctatctgcga tttgtaagat tgatttttct tctcaagttc cttgttcttc taccgatatt
    43621 acctcgtaca gtgacgagga ctgaacaatg gccactatct cttcaacttc aattccttcc
    43681 atacaaacac agagtagtaa cagggcatca caaggtagcg atgttgcttc acaaatcgcg
    43741 cggataagtc agcaaatcat caaactcact caacaaatta aagagattgt agacactagc
    43801 ggcagtgctg aagacaagca aaaacaggcc gaattgattc aacagcaaat caccctactg
    43861 gaaacacaac tggcgcagtt gcagaaacag caggcagaaa aggcgcagga gaaagagcag
    43921 cgtttgtctc tgaatgtatc ccttctaaat cctgttgaaa aaacgactca tatcgatatc
    43981 tatatctgat acaacggatc cccttcccga ccagccatta gcgcattgcc ctggtctcgt
    44041 accacgtccc ataacgcttc agcggcggtg gaaagtgaac ggttcttcct gcgcaccagc
    44101 atcaactggc gttcaaccac cggcgtaatg cgcttaacca ccagtgggct accttcaggc
    44161 aatggtagcg ccagtgcagg aagaatactg atgccgatgc ctgcggctac catcgggaac
    44221 agcgtcgccg gatgtccaat ctcctgcaca atattcgcct gaataccatt gcgcgccagc
    44281 gctgcgtcaa tcagcggtcg gctgcctgac gcgtaatcct gcaacaccaa tttcgctccc
    44341 tgtaatgcct gccagggtac ataatcttcc acggccaaag cgctatcgcg atggcaaagc
    44401 agaaagaaag gctcggaaag aatcgcttca cattgcagat cgcccactgg gcctggatcg
    44461 atgacgatgc caaagtcgac atctccctgg cgaatacttt ccatcaccca ctgctgcgga
    44521 cgatcatgca ggacaaactg aatatctgga tagcggcgat ggctttcagc aatgcattgc
    44581 ggaataagat gcgcggaaat ggtctggctg gcagcgaccc gaacttttcc gctcagttgt
    44641 tgccccatac gcccggtatc gcgcaacgtg ctgttaagtt catccagcag tcgctcaaga
    44701 cgcaaagcca actgctgccc tgcatctgta agcaccactt cacgcgtggt tctgtccagc
    44761 aggcgaacac cagtatgatt ttccagctcc ttcacactgt gacttactgc cgactggctc
    44821 aagccgatac gctctcctgc acgactaaaa cttttctcct gcgctactgt gacgaaaacc
    44881 tttaattgtt ttaaagaata attcatctgt tttcttcatg aatggatgca ataaatcaat
    44941 tttatttctc aaactggaag aagcacaata gaaccatcga tcatctggag tctttatgaa
    45001 actttttcgt atcctcgatc ctttcacctt aaccctgatc acggtggtgt tgctggcctc
    45061 tttctttccg gccagaggcg atttcgtccc cttctttgaa aatctgacca ccgcagctat
    45121 tgccctgctg ttctttatgc acggcgcgaa gttgtcgcgt gaggcgatta ttgctggcgg
    45181 tggtcactgg cgactgcatt tgtgggtaat gtgcagcacc ttcgtgctgt ttccgattct
    45241 gggtgtactg tttgcctggt ggaaaccggt aaatgtcgac ccgatgctct actccggttt
    45301 tctctacttg tgcattctcc cggctaccgt gcagtctgca atcgccttca cgtcaatggc
    45361 gggcggtaac gtcgcggcgg cggtttgttc tgcgtcagca tccagcctgc tggggatttt
    45421 cctttcacca ttgctggttg gtctggtgat gaatgttcac ggtgcaaggg gcagccttga
    45481 gcaggtcggt aaaattatgc tgcaactgct gctgccgttt gtgttggggc atctttcccg
    45541 gccgtggatt ggtgactggg tgtcgcgcaa taaaaaatgg attgcgaaaa ctgaccagac
    45601 gtccattctg ttggtggttt atacagcgtt cagcgaagcc gtcgttaatg gtatctggca
    45661 taaagttggc tggggatcat tgctgtttat cgtggtggtc agctgcgttc ttctggctat
    45721 cgtgattgta gttaacgtct ttatggcacg ccgactgagc ttcaataagg cagatgaaat
    45781 tactatcgtc ttttgtggtt cgaaaaagag tctggcaaat ggcatcccga tggcaaacat
    45841 tctgttcccc acatcggtga tcggtatgat ggtgctgccc ctgatgattt tccatcagat
    45901 ccaattgatg gtctgtgcgg tgctggcgcg tcgatacaaa cgccagaccg aacagttaca
    45961 ggcgcagcag gaaagcagcg ccgataaagc ttaaagcgga cgcttcaggg gctggaccag
    46021 ttgcgtcagc ccctcggttt taatcagcag cgtgatttgc attagctcac cgagcttccc
    46081 tgccgggaac tcatctttgc gggcaaacca cagcagatac tcctccggca ggtcaattaa
    46141 gcgacgccct ttgtatttgc caaacggcat tatcgtattg gctatttcaa tcagctgctc
    46201 tttttccatc tcagctaccc agcaaacgca gcatttccgc ttcgtcgatg acttcaatgc
    46261 ccagttcctg cgccttcgcc agtttagatc ctgcagcttc acccgctatc accagatcgg
    46321 ttttcttcga cacgctgccc gcgactttcg cgcccagttc gaccagtcga gctttagcgt
    46381 catcacgcga catctggctt aagctgcccg taagcaccac ggttttacca gcaaacgggc
    46441 tgtcaatctc ttccgcgttg ataacgatcg gcgcaggcca gtgaacacct tccgccaaca
    46501 gctcgctgat gacattgcgg ttgctttctt cggcaaagaa gttgtgaacg tgggatgcaa
    46561 cgacaatgcc aacatcaggc accttttgca gctcttcaat cgaagcggct tccagcgctt
    46621 ccagcgtgcc gaaatatgcc gccagacctg ctgcggtggc ctcgccgact tcacggatgc
    46681 caagtgcata gaggaagcga gcaaaggtgg tttctttcgc tttttccagc gcgttaacca
    46741 cgttttgtgc caattttggc cccatacgct ccagtccggt cagtttgcct gcggtgagtt
    46801 tgaacagatc tgccggagta tggacatatt ctttttcaac cagctgatcg atgattttgt
    46861 cgcccattcc gtcaacatcc atcgcacggc gggaaacaaa gtgtttcagc gactctttac
    46921 gctgcgcacc gcaaatcagg ccaccggtac agcgggcaac cgcttcacct tccacacgct
    46981 caacgtcaga accacatacc ggacaatgcg tcgggaatac aacctcacgg gtatcttccg
    47041 ggcgttcaga aagcacgacg ttaaccacct gcgggatcac gtcgccagcg cggcgaatca
    47101 ccactttatc gccaatgcgt aaaccaagac gttcgatttc atccgcattg tgtaaggttg
    47161 cgttactcac cagcacgcca gcaacatgga caggttccag acgcgcaaca ggcgtaatcg
    47221 cgccagtacg cccaacctga aactcgacgt cacgcacaaa ggtcatctgc tcctgcgccg
    47281 ggaatttaaa cgcaaccgcc cagcgcgggg cacgcgcgac aaagccaagc tgctcctgct
    47341 gttccagtga gttgacctta atcaccacgc cgtcgatatc aaagcccagc gtcgggcggt
    47401 cttcttccac tttgtgatag aacgccagca cttcttccgc cgattcacaa agcgttaccc
    47461 gatcgctgac cggcaccccc cactttttaa attgcagtaa acggccaaga tgagtatccg
    47521 gcagctcgcc accttccaga acaccaacgc catagcagaa aaaagtgagc ggtcgcttcg
    47581 ctgtaatacg cggatcaagc tgacgcagtg aaccagctgc cgcattacgt gggttagcaa
    47641 aaactttccc gcccgtgcgt cgcgcatctt cgttaatctt ttcgaacccc gcctgcggca
    47701 ggaacacttc accacgcact tccagacgcg ccgggatatt ctctccgtgc agcttcagcg
    47761 gaatggcgcg aatagtacgc acattagacg tgatatcttc cccggtggtg ccatcgccac
    47821 gggtcgcggc actgactaaa acgccatttt catacagaat actgacggca aggccatcca
    47881 gcttcagctc acagcaccag gtgactttct cgttgttttt cagacggtcc tgcacacgtt
    47941 tgttgaaagc aagaaagctt tcttcatcaa aaacgttatc cagtgacagc attggtactt
    48001 catggcgtat ctggctgaaa gctgccagcg gcgcagcacc tacacgttgg gtaggcgaat
    48061 caggcgtaat cagttctgga tgtttggttt ccagctcgcg cagttcgcgc atcagcctgt
    48121 cgtattcagc gtcgggaatt tccggcgcat ccatcacatg ataaagatat tcatgatggc
    48181 aaagcgtcgt tcgcagttct gtcagttgtt gttcgattga ttccatatcg caccatcaat
    48241 gctaaaaacc cccgacaagc gggggttcga agaggagtta atttgcctta agtgtatcag
    48301 gcgttggcgt ctttgacttc gcggatgatg tcctggtact cgcgcaattt ctgcggagtc
    48361 atcatacggc gctgatcgtc aagcacgaca ccgcccactt catcggcaat atgctgcgca
    48421 gattgcagca tcagcttgaa gttctgcagc tcgtcaccgt aagacggtac ctgcataaag
    48481 atagtgacac ccggagtagt gaaatccttc atttcaggat caaaggttcc cggtttcacc
    48541 atattcgcca ggctgaataa cgccgggccg ctgccatccg ggctaagatg acgatggtaa
    48601 atattcatat cgccaaaaat gaagcccgct tgttgaatgc tgttaagaag cagttcaccg
    48661 tttagctcgc taccgtgatg cgccgcgacg ttcatgataa tcaccgcttc tttgcgcttc
    48721 ggtttatcca taactggagc cggttccgct acaggctcag gctgtggtgc cgctacgggt
    48781 tctgcaggct ggaaagcctg ttgtgccggt tgcggtgctg aatgcacagg ctgcggcgct
    48841 ggcgcgacct gtggcgaaac tggctgctgc aacggctgtt caggctgcgg ctgataggca
    48901 ggctgctgca ccggctgcgc tggacgcgga gcatgttgcg gcggtacctg cgcttcaggc
    48961 ggctgctgga ccggttgacg cggctgcgca gacgcataag gcggtaggta ctggtgttgc
    49021 ggcgacggac gagcagcctc atgctcctga gcgttagccg gggcatgatt cacgcggtga
    49081 acacgaacct caccaacgcc ctcatcatct tcgacatcct cgtcataaga atcgtcgtca
    49141 cgttttgact tcattcgttt taatggccga tcgcggaaca tagaagatcg ttctttacgg
    49201 ctggtccaga aaccatgtac cagtaaagcg attatggcga tcgcgccaac aatgattaat
    49261 atcagacgca aatcctgcat cattatattc tctgttgttc taacaccttg ccaccacggc
    49321 aaacatttac tcactaagag tatttgccga ttacctcaag tgcaagtgca ctattaactt
    49381 tcacagcaca aagatagatg aaatcgtgct ttttgctgtt ttttcgaaca tatcctaact
    49441 gtccattgcg caattacccg gtaaaatacg cagaattttc ctgggattgg tcaaaaggag
    49501 ctcatcctga ttatggtttc atcattcaca tctgccccac gcagcggttt ttactatttt
    49561 gcgcaaggct ggaagctcgt ctcgcaacct gggattcggc gtttcgttat tttaccgctg
    49621 ctggtcaata ttttgttgat ggggggcgca ttctggtggc tctttacaca gctcgatgtc
    49681 tggatcccga ctttcatgag ttacgttccg gactggctac aatggctgag ttatctgttg
    49741 tggcctctgg ctgtcatctc tgtgctgtta gtgtttggct atttcttctc cacgattgct
    49801 aactggattg ccgctccgtt taacggttta ttggctgaac aactggaagc acgattgact
    49861 ggcgctacac cgccagatac cgggattttc ggtatcatga aagatgtgcc gcgaatcatg
    49921 aaacgcgaat ggcaaaaatt tgtctggtat ctgccgcgcg caattgtatt actaattctt
    49981 tacttcatcc ctggtattgg gcaaaccgtc gcgccggtac tgtggttcct gtttagcgcc
    50041 tggatgttag ccatccagta ttgcgattac cccttcgata accacaaagt gccgtttaaa
    50101 gagatgcgca ccgccctgcg cacacgcaaa atcaccaata tgcagtttgg tgctttaacc
    50161 agcctgttta cgatgatccc gctgcttaat ctgttcatca tgcccgttgc cgtttgtggc
    50221 gcgacggcga tgtgggtcga ttgctatcgc gataaacacg cgatgtggcg gtaacaatct
    50281 accggttatt ttgtaaaccg tttgtgtgaa acaggggtgg cttatgccgc cccttattcc
    50341 atcttgcatg tcattatttc ccttctgtat atagatatgc taaatcctta cttccgcata
    50401 ttctctgagc gggtatgcta cctgttgtat cccaatttca tacagttaag gacaggccat
    50461 gagtaagatt tttgaagata actcgctgac tatcggtcac acgccgctgg ttcgcctgaa
    50521 tcgcatcggt aacggacgca ttctggcgaa ggtggaatct cgtaacccca gcttcagcgt
    50581 taagtgccgt atcggtgcca acatgatttg ggatgccgaa aagcgcggcg tgctgaaacc
    50641 aggcgttgaa ctggttgaac cgaccagcgg taataccggg attgcactgg cctatgtagc
    50701 tgccgctcgc ggttacaaac tcaccctgac catgccagaa accatgagta ttgaacgccg
    50761 caagctgctg aaagcgttag gtgcaaacct ggtgctgacg gaaggtgcta aaggcatgaa
    50821 aggcgcaatc caaaaagcag aagaaattgt cgccagcaat ccagagaaat acctgctgct
    50881 gcaacaattc agcaatccgg caaaccctga aattcacgaa aaaaccaccg gtccggagat
    50941 ctgggaagat accgacggtc aggttgatgt atttattgct ggcgttggga ctggcggtac
    51001 gctgactggc gtcagccgct acattaaagg caccaaaggc aagaccgatc ttatctctgt
    51061 cgccgttgag ccaaccgatt ctccagttat cgcccaggtg ctggcaggtg aagagattaa
    51121 acctggcccg cataaaattc agggtattgg cgctggtttt atcccggcta acctcgatct
    51181 caagctggtc gataaagtca ttggcatcac caatgaagaa gccatttcta ccgcccgtcg
    51241 tctaatggaa gaagaaggta ttcttgcagg tatctcttct ggagcagctg ttgccgcagc
    51301 gttgaaacta caagaagatg aaagctttac caacaagaat attgtggtta ttctaccatc
    51361 atcgggtgag cgttatttaa gcaccgcatt gtttgccgat ctcttcactg agaaagaatt
    51421 gcaacagtaa tgccagcttg ttaaaaatgc gtaaaaaagc acctttttag gtgctttttt
    51481 gtggcctgct tcaaactttc gcccctcctg gcattgattc agcctgtcgg aactggtatt
    51541 taaccagact aattattttg atgcgcgaaa ttaatcgtta caggaaaagc caaagctgaa
    51601 tcgattttat gatttggttc aattcttcct ttagcggcat aatgtttaat gacgtacgaa
    51661 acgtcagcgg tcaacacccg ccagcaatgg actgtattgc gctcttcgtg cgtcgcgtct
    51721 gttaaaaact ggcgctaaca atacaggcta aagtcgaacc gccaggctag actttagttc
    51781 cacaacacta aacctataag ttggggaaat acaatgttcc agcaagaagt taccattacc
    51841 gctccgaacg gtctgcacac ccgccctgct gcccagtttg taaaagaagc taagggcttc
    51901 acttctgaaa ttactgtgac ttccaacggc aaaagcgcca gcgcgaaaag cctgtttaaa
    51961 ctgcagactc tgggcctgac tcaaggtacc gttgtgacta tctccgcaga aggcgaagac
    52021 gagcagaaag cggttgaaca tctggttaaa ctgatggcgg aactcgagta atttcccggg
    52081 ttcttttaaa aatcagtcac aagtaaggta gggttatgat ttcaggcatt ttagcatccc
    52141 cgggtatcgc tttcggtaaa gctctgcttc tgaaagaaga cgaaattgtc attgaccgga
    52201 aaaaaatttc tgccgaccag gttgatcagg aagttgaacg ttttctgagc ggtcgtgcca
    52261 aggcatcagc ccagctggaa acgatcaaaa cgaaagctgg tgaaacgttc ggtgaagaaa
    52321 aagaagccat ctttgaaggg catattatgc tgctcgaaga tgaggagctg gagcaggaaa
    52381 tcatagccct gattaaagat aaacacatga cagctgacgc agctgctcat gaagttatcg
    52441 aaggtcaggc ttctgccctg gaagagctgg atgatgaata cctgaaagaa cgtgcggctg
    52501 acgtacgtga tatcggtaag cgcctgctgc gcaacatcct gggcctgaag attatcgacc
    52561 tgagcgccat tcaggatgaa gtcattctgg ttgccgctga cctgacgccg tccgaaaccg
    52621 cacagctgaa cctgaagaag gtgctgggtt tcatcaccga cgcgggtggc cgtacttccc
    52681 acacctctat catggcgcgt tctctggaac tgcctgctat cgtgggtacc ggtagcgtca
    52741 cctctcaggt gaaaaatgac gactatctga ttctggatgc cgtaaataat caggtttacg
    52801 tcaatccaac caacgaagtt attgataaaa tgcgcgctgt tcaggagcaa gtggcttctg
    52861 aaaaagcaga gcttgctaaa ctgaaagatc tgccagctat tacgctggac ggtcaccagg
    52921 tagaagtatg cgctaacatt ggtacggttc gtgacgttga aggtgcagag cgtaacggcg
    52981 ctgaaggcgt tggtctgtat cgtactgagt tcctgttcat ggaccgcgac gcgctgccca
    53041 ctgaagaaga acagtttgct gcttacaaag cagtggctga agcgtgtggc tcgcaggcgg
    53101 ttatcgttcg taccatggac atcggcggcg acaaagagct gccatacatg aacttcccga
    53161 aagaagagaa cccgttcctc ggctggcgcg ctatccgtat cgcgatggat cgtaaagaga
    53221 tcctgcgcga tcagctccgc gctatcctgc gtgcctcggc tttcggtaaa ttgcgcatta
    53281 tgttcccgat gatcatctct gttgaagaag tgcgtgcact gcgcaaagag atcgaaatct
    53341 acaaacagga actgcgcgac gaaggtaaag cgtttgacga gtcaattgaa atcggcgtaa
    53401 tggtggaaac accggctgcc gcaacaattg cacgtcattt agccaaagaa gttgatttct
    53461 ttagtatcgg caccaatgat ttaacgcagt acactctggc agttgaccgt ggtaatgata
    53521 tgatttcaca cctttaccag ccaatgtcac cgtccgtgct gaacttgatc aagcaagtta
    53581 ttgatgcttc tcatgctgaa ggcaaatgga ctggcatgtg tggtgagctt gctggcgatg
    53641 aacgtgctac acttctgttg ctggggatgg gtctggacga attctctatg agcgccattt
    53701 ctatcccgcg cattaagaag attatccgta acacgaactt cgaagatgcg aaggtgttag
    53761 cagagcaggc tcttgctcaa ccgacaacgg acgagttaat gacgctggtt aacaagttca
    53821 ttgaagaaaa aacaatctgc taatccacga gatgcggccc aatttactgc ttaggagaag
    53881 atcatgggtt tgttcgataa actgaaatct ctggtttccg acgacaaaaa ggataccgga
    53941 actattgaga tcattgctcc gctctctggc gagatcgtca atatcgaaga cgtgccggat
    54001 gtcgtttttg cggaaaaaat cgttggtgat ggtattgcta tcaaaccaac gggtaacaaa
    54061 atggtcgcgc ctgtagacgg taccattggt aaaatctttg aaaccaatca cgcgttctct
    54121 atcgaatctg atagcggcgt tgaactgttc gtccacttcg gtatcgacac cgttgaactg
    54181 aaaggcgaag gcttcaagcg tattgctgaa gaaggtcagc gcgtgaaagt tggcgatact
    54241 gtcattgaat ttgatctgcc gctgctggaa gagaaagcca agtctaccct gactccggtt
    54301 gttatctcca acatggacga aatcaaagaa ctgatcaaac tgtccggtag cgtaaccgtg
    54361 ggtgaaaccc cggttatccg catcaagaag taattcttgc cgcagtgaaa aatggcgccc
    54421 atcggcgcca ttttttatgc ttccgccagc ggcggcaaaa tcaattcatc gctctcatgc
    54481 tgctgggtgt agcgcatcac ttccagtacg cgcaaccccg cacggtgcac cgcatcgttt
    54541 aacgccttcc ctttcagcaa accactgata agctgagcac aaaacaggtc gccagtccct
    54601 ttcaggtcgg tttttacccg tgaatgggaa atgacattca cgctgtcggc actgaccacc
    54661 accacctgca tctcctgatt ttcttcatta ccggaggcgc tggtaatcac cacccatttt
    54721 aatgtgtctg aaagcagact ttttgcggca gcaatggcac tgtcgagatc gcggcaattt
    54781 ttaccggtca ggatttccag ctcaaagata ttgggggtaa ttccctgcgc cagcggcagt
    54841 aaatattgtc gatacgcctc gggaaggtca ggtttgacat aaattccgct atcaatatcg
    54901 ccaatcaccg gatcgaccat gatcaatagg tcaggatggt ctttgcgtag cgcagtcagc
    54961 cactcggcaa ggattttgat ttgcgatgcc gttcccatat agcccgtggt tacagcacga
    55021 agttggcgca gcgcatcacg ctcctgaagc gcacgcaaat agccgctaaa ccattcgtcc
    55081 ggaatcgcac caccgtagaa agtgtcataa tgcggcgtat tgctcagcaa taccgtcggc
    55141 acggcaaaga cattcaagcc gttctgtttt atagcaggca ccgcaatgct gttgcctacg
    55201 cttccgtaca ctacctgcga ctgcacggcg acgatatccg cctgcagcgc cctactctta
    55261 tcgttaaaca acaacaaact actcatttaa ttttttctcc ttgccgatga tcctcatcgt
    55321 attccaaccg aaactttacc tgattctggc agtcaaatcg gctattacaa aacaaagata
    55381 aggtaattca gtgaagaaaa tcatttgtct ggccattacg ctgctaatga cactccccgc
    55441 ttacgcgaag ttaactgttc atgaagaagc ccgcatcaac gccatgctgg agggattagc
    55501 acagaaaaag gatttgatat ttgtgcgcaa cggtgatgaa catacctgcg atgaagcggt
    55561 ttcccacctg cgtctgaagc tcggcaatac ccgtaaccgc attgacactg ccgagcagtt
    55621 tattgataag gtagcttcgt cgtcatcgat taccgggaag ccgtatatcg tgaagatccc
    55681 cggtaagagc gatgagaacg cacagccttt tttacatgcg ttaattgcgc agacggataa
    55741 aacggtgcct gcggaaggaa attaatccgc tttggtaagg catttacagg aggtaacatg
    55801 aaaaaatgct ttatttatca cgatgaaaaa tcgaataaat tttggtggat agattacgaa
    55861 ggggatagtt tagctgtcaa ctatggcaag gtaggtagta ttggtaaatt ccatacaaaa
    55921 gaattcgata atgaagaaca gtgtctgaaa gaagccagta aattgattgc cgcaaaaatg
    55981 aaaaaaggat atcaagaaac tccagacttt aacttcatgg atctctacta ttttgatgat
    56041 gaagagattg ggttacatct taaaacgtca cacccaaact tccagtgcca ttttactgat
    56101 ccactttata tgtgttgctg ggatgaagaa tctccttttg gtagcgatga aggtgctgat
    56161 gctctaaacg aacttgaaaa tagcctccgt tctggactgt gctgatttcc ctcaaatgtt
    56221 aattgaaact atgtggggta tgaaatacat cgctatggac agtattcttg aagaggatgt
    56281 gcgtgcgcaa ttactagccg atgaaatgag ctctatccag agcaatatga ttacctacgc
    56341 gactgcattc ggtcagatca aagtcatggg taaaatctcc cataaactta aaaagatggg
    56401 actcaatgca ctagcgcgtc atcagcttac cgcaaaaatt cttcaatggg gtgacggtca
    56461 ggactcacca atacttcaaa aaatgattga tgaccttact gcttttccac acgaaaatta
    56521 aaaactgcat ttgtcggcag caacaactgc cgacaaatca gattattgac aaagtgcact
    56581 ttgtttatgc cggatgcggc gtaaacgcct gatccggcat aaaaatattg ctaattcaat
    56641 atattgcaga aacattgtag gcctgataag cggagcgcat caggcagttt tgcgtttgtc
    56701 atcagtctcc aatgctatta atccttaaat ccctgccccc tgactaaaat gctcttcccc
    56761 aaacactccg gtggagaggt agcgatcgcc acgatcgcag atgatcgcta ccaccaccgc
    56821 accagggtta gcttttgcca cccgcagtgc tccagcaacc gcgccgcctg agctgacgcc
    56881 acagaatatt ccttcccgca ccgccagttc gcgcatggtg ttttccgcat cgcgctgatg
    56941 aatatccagc acctcatcca ccagcgcagc gttgaaaatc cccggcagat attccgcagg
    57001 ccagcggcga atgcccggaa tactgctgcc ttcttccggt tgcaggccaa caatggtcac
    57061 cggtttggat tgttcgcgca taaagcatga aacgccggtg atagtgccgg tcgtccccat
    57121 gctggagaca aaatgagtga tgcgcccgcc ggtttgctgc cagatttccg gcccggtggt
    57181 ggtgtaatgc gcataagggt tatcgggatt attgaactga tcgagcagct ttccttcgcc
    57241 acgattcgcc atctccagcg ccagatcgcg cgcaccttcc atgccctgct ctttggtgac
    57301 aagaatcagt tccgcaccat aagcacgcat cgccgcacgg cgttcctggc tcatgttgtc
    57361 gggcatcagc aatttcatgc gatagccttt cagcgcggca atcattgcca gcgcaatgcc
    57421 ggtgttacca ctggtggctt cgattaagac atcacccggt ttaatttccc cgcgcttttc
    57481 cgcctcgacg atcatcgaaa gtgccgcacg atctttcacc gaacctgccg ggttattgcc
    57541 ttccagtttt aaccacactt cactgccgtt atccggcccc attcgctgca acttcaccag
    57601 aggcgtattg cctattgttt gttctaatgt actcacaatc tctatccata cgtggtgttg
    57661 cctgatgcga cgcttgcgcg tcttatcagg cctacaggtt acaaaccttg ccattaaaaa
    57721 gcccggatcg cgggaagcgc ctccgggcgt ttaacattca ctcaacctat caggcgcttt
    57781 gtgcgagagc aagttcctca tcgcgggttt caatacgctc gtcgccgtta tacagccgcg
    57841 catgttgcag accaacgaat aaacgctcgc catgctgcgg ggcatcgtcg ccatgcatca
    57901 cgaccgtcag cggttcgttg taccacccca gcggctgcac cactaattgg gtgtagtgac
    57961 ctttcgggct ggcttccagt acctgtaccg gcagcggcga atcgagactg gtacggcggc
    58021 tgatatccac ttcccacggg cgcaggaaga gatccaccgg cccctgatac gcaggtgtgt
    58081 agcccagcgg ccagcgatgc gcgccaacat ggaactgccc gccgcgaatg gttccctgca
    58141 ggcggttcac ttcgcccata aattcgagta caaaacgggt cgctggttcg cgccatacct
    58201 gattcggcgc atcagcctgt tcaatattcc cctgactcat caccactaca cgatcggcta
    58261 cttcggtcgc ctcttcctgg tcgtgggtca caaacacgct ggtgaatttc agctcttcat
    58321 gcagttgacg cagccagcga cgcagctctt tacgcacctg cgcatccagc gcgccgaacg
    58381 gttcatcaag cagcagaatt tgcggttcta cagccagcgc gcgcgccagc gccacacgct
    58441 gtttctggcc gccggaaagc tgcgccggat aacgatccgc cagatgcgca agctggacca
    58501 tttccagcaa ttttgtcact ttcgctttga tggctgcggc attcgggcgc tcgcgacgcg
    58561 gcagcaccgt caggccaaaa gtgatattgt cgaataccgt catatggcgg aacagcgcgt
    58621 aatgctggaa cacgaaaccg actttacgat cgcgcgcgtg caggcgactc acgtcggtgc
    58681 cgtggaagcg aatatgcccg ctggtttgat gctccagccc ggcgataatc cgcagcagcg
    58741 tggttttccc ggaaccggac ggccccagca acgcgaccat ctgaccagaa ggaatatcca
    58801 gtgagatatc gttcagcacc tgggtgcgac caaaagactt cttgatattg gcaatctcaa
    58861 tgctcatgat gctcctcctg ctgcgcgcgt ttttcctgat tctccaggcg ccactgcaac
    58921 atactcttta aaaacagggt gataatcgcc atcaacgtca acagcgcggc ggcggtaaag
    58981 gagccgacgg tgttgtagtc ctgctccaac aattcaatct gtaacggcag cgaaagtgtt
    59041 tcgccgcgaa tcgagccgga aaccaccgac actgcaccaa actcgccaat cgcgcgggcg
    59101 ttggtcagca ccacgccgta aagcagcgcc cagcgaatat tcggcaatgt gacgcgacgg
    59161 aacatctgcc agccggacgc gccaagcaaa atcgccgctt cgtcttcctg gctgccctgg
    59221 cttaacatca ccggcaccag ctcgcgcacc acaaacggac acgtcacaaa gatggtgacc
    59281 agcaccattc cgggccagga gaacataatt tgcagattat gttcatccag ccagccgccg
    59341 agcgggccgt tggagccgta gaacagcaga tacaccagac cggcaaccac cggcgataca
    59401 gcaaacggaa tgtccagtag cgtcagcagt aactggcgtc ccgggaagtt aaagcgcgtc
    59461 accagccagg ccagcagaat gccgaacacc aggtttaccg gtacggcaat cagcgcgatc
    59521 atcaccgtca gccagatggc gtgcagcatg tccggatcgg ccagattctg taaaaccggc
    59581 atcagcccct tgctgaatgc ctgcacgaag atgtaaatca tcggcaccag caggatgaac
    59641 gccgaaacca gcatcccgat gccaatcaga aaccatttgc cccagttaat cgggcgcgcg
    59701 tcataacgct tcaattgggt aacttccgcc attaatgacc taccacacgc cgaccaaagc
    59761 gactttgcag agtgttaatt gagaacagca gcagcagaga tgccgcgagg atcaccgaag
    59821 caatcgcgct cgctgccggg taatcaaact cctgtaagcg cacaaaaatc atcagcgacg
    59881 tcacttccgt cttccacgcg atatttccgg cgataaaaat caccgcgcca aattcaccaa
    59941 gactacgggt aaacgacagc gccacgcccg ccaccagcgc cggagaaagc tccggcagca
    60001 ccactttgca gaaactctgc cagcgcgttg caccaagcgt ttccgccgct tcttcatatt
    60061 ccgggcctaa ctcttccagc accggctgca cggtacgcac cacaaacgga atgctggtaa
    60121 aggccatcgc caccgcaata ccaagccagg tataggtgac tttgatatca aacttcgcca
    60181 gccattcacc gtaaaaaccg tttacggaaa agagcgaggc cagcgttaaa ccggcgactg
    60241 ccgttggcag cgcaaagggt aaatccatca gcgcatcaag cagcgtgcgg cccggaaagc
    60301 gataacgtgt taaaatccac gccatcagca gaccgaaaac gccgttaaaa atcgatgcca
    60361 caaacgccga cagcagcgtc actttgtagg ccgcgaccac ctgcgggttg gtgatcacct
    60421 cccagtactg cgcccagctc atctcagaaa gctgcatcac cagcgcagag agcggcagca
    60481 gcaatatcag gcacacaaac agcagactgg tgccaaggct taaagtaaag cccggcagca
    60541 cgcgtcttga agaaactgca aacattactt acgccccgcc gctaacagct tgtctaactc
    60601 gccgccgctg gtgaagtggg ttttcatcac ttccggccat gagccaaatt tgtcttccac
    60661 gcggaacagt tcggtctgcg ggaatttatc tttcagtttg tccatgactt ccgggttatt
    60721 cacacggtag taatagtcgg tgatgatggt ctgcgcctgt gggctgtaga gccagttcag
    60781 gtaggctttt gcggcttttt ccgtaccgtt ggcctgcaca tttttatcga cccacgccac
    60841 cgggaattcc gccagaatat tggttttcgg gatcaccact tcaaagccct gtgcttcata
    60901 ctgtttacgg atgttgttca cttccgattc aaagctaatc agcacatcgc ccaagccacg
    60961 ctcggcaaaa gtggtggttg cgccacgacc gccggtatcg aacacttcaa cgtttttcag
    61021 gaactgggtc ataaactgtt cggttttggc tttatcgcca ccgtcagctt tatccgctgc
    61081 gccccatgcc gccagatagg tataacgcgc gttacccgac gttttcgggt tcgggaaaat
    61141 cagcttcacg tcggagcgca ccaggtcgtt ccagtcgtgg atattcttcg ggttgccctt
    61201 acgcaccagg aagcccatgg tggagtagaa cggcgagcta ttattcggca ggcgcgactg
    61261 ccagtcagcc gggatcagct tgcctttgtc gtgcaggatt tgtacgtcgg tcacctggtt
    61321 ataagtgaca acgtctgctt ttaagccctg caaaatcgcc agcgcctgtt ttgatgaccc
    61381 ggcatgagat tgttttatcg tcagtttgtc gccgccgtta tcttttgccc attgctgttc
    61441 aaacggcgga ttcagggcgg caaacagctc gcgggagacg tcataagaac tgttcagcag
    61501 ttccgttgcc tgtacatggc ccgccagcag cagagaagcg accagcgcga gtgagttctt
    61561 tttcagtaag ttaacggcca ttgcgcaccc ttataaattt aatgactttc taatagccat
    61621 catatttata acggacgtga aaggagtaac ggttttatat accgtttggt gatttggaag
    61681 ttaaaaaggg aataagaact tggttcaaca ggcgtaattg ttgcaggcag tttggacacg
    61741 gacagcgcgg agaaaccgga gcgtacacat agtacgtgag gatttcgagc actgcccggg
    61801 gccaaaatga caaatgaaat agcctggtga acttagttca agacgaaatc ctccccacac
    61861 atgcagggag gaaacagagg tgaatcagat accgacgcta accgtctccg gcagtgtgct
    61921 gccgccatca atcacattct gtgtaccggt taaatagctg gattcatccg atgcgaggaa
    61981 ggccgccagt tcgccgactt ccagcggatc ggcgaggcga cacagcggga ttgcttttgc
    62041 catttcagtc agcaccgatt ctggatcttc cgggttcgac tggcgggcaa tgctttccgc
    62101 cattggcgtg cggacgtatc ccgggcagat ggcgttaacg cgaataccgg attgcgcgta
    62161 ctccaccgcc agcgatttag tcaggccaac aatcgccgct ttcgttaagg catacgccgt
    62221 ttcgccagga tcggccacca tatcaccagt gactgaagac atcatcacaa tgcgaccatc
    62281 tttgcgcgca atcatctccg gcagcaccgc cttcgtgacg ttccatacgc ctttaatatt
    62341 gatatcaata tggaaatcgc gatcttcatc gctcatatcg aggaaactgc ccagacgaca
    62401 aacgcctgcg ttattcacca ggatatcaat gcgcccttct ttttccttcg cgcgtttgat
    62461 agctgcggct accgacgccg ggtcacgcac atcggcgaca accgccgtac agcgatgacc
    62521 acgaccacac agttcgtccg ctagcttttc gatctcaggg gagatatcca gcaagattag
    62581 gttcgcgcca tgacgtgcaa aagttctggc aattccttcg ccaattccct gcaatgcgcc
    62641 cgtaatcagt gctgtcttgc ccgtgagttt acccatttta atgctctcct tgttaataca
    62701 gagcgttaca ctccattccc tctgaaaata caggataaac aaaatattgc tgcggatcag
    62761 ctcactaatt caggaaaatc tcaatccagt cgctgcttta tgcatcattt caggcgttgt
    62821 gttagctcac tgctgcgctg aatcattttc aatgacgcca catcattgat gttgcacaag
    62881 accaacaaac agtaaatcgg taacagaatt ttgcgccgtg cgggtagaca tcgacgagct
    62941 acgccattcg gtttcaccgg aaacggtatc gagcgtgaaa tgcgccagcc gccgcagcgg
    63001 agagtcagcc agagaagtaa tggcaatcac cgttgccccc tgctttcgtg ccgcttctgc
    63061 gcacaagaca atctcttttt tactgccgct gtaagagata gcgatttgta catcgccttt
    63121 tttcaacgcc tgggaaacgg tggcttgtac gtgcgtgtcg gcttcgcagg ccacgcgata
    63181 gccaattttc atcagtttga acgacagatc gcgccccacc agcgccgatc cgcccaggcc
    63241 cgttatctgg ataaacggtg ctttactgat gacctcaatg attttttgca gccgcgcgta
    63301 gtcgaacaac gcacaggttt gctccagcgc cagctctttt tcgcgattca gtttgcgggc
    63361 aatcacttcc agcgaatcat cactggtaat cgaactgtgc aggtgcagcg ccgtggcatt
    63421 cgttttctcc cggctggcac tgtattcgcc aattaacgcc atacgcaatt cagtaaatcc
    63481 ttgcgcgccg agcttctggg caaatttcac aatgctcgac tggctgatac cgagctgttt
    63541 cgccatctgc cgtgaagaga cggatttcag ctcgctcact ctggcccgca gaaagtcagc
    63601 aattttttgc tcgttcccgg taaattcact ttccgcattg cgtattttcg tcaggtacaa
    63661 catcttccac tctcctgggc tggcagcaaa aattccgctt aagttcttat gacgctcttc
    63721 acactctgcg agtgtagact caatgattcc tttaagtctg tcaattcggg atatatgatt
    63781 ccatacatct gcaattattt gatcgtaaat agtaaggtca ccaccgatgc aactagaaaa
    63841 gatgattact gaaggctcga acaccgcctc ggctgaaatt gaccgcgtat cgacgctgga
    63901 aatgtgccgg attatcaacg atgaagataa aaccgtaccg cctgccgttg atcgcgtcct
    63961 gccggatatc gccgcggcga tcgatgttat ccacgcccag gtcagcggcg gcggtcgtct
    64021 gatttatctc ggtgcgggaa catccggtcg tctggggatt ctggatgcca gcgaatgtcc
    64081 gcccacctac ggcgtgaaac cgggtctggt ggttggtttg attgctggag gcgaatatgc
    64141 cattcagcac gcggtggaag gagctgaaga tagccgggaa ggtggtgtta acgatctgaa
    64201 aaatattaat ttaacggcac aggatgtggt ggttggcatt gctgccagcg gtcgcacgcc
    64261 gtatgtgatt gccggactgg aatacgcacg ccagctcggc tgccgcacag tgggaatttc
    64321 ctgtaatccg gggagcgccg tttcaaccac cgctgagttt gccattacac cgattgtagg
    64381 tgccgaagtt gttaccggtt cttcgcggat gaaagcaggt acagcgcaga aactggtgct
    64441 caatatgctt tccaccgggc tgatgattaa atccggcaaa gtgttcggca acctgatggt
    64501 cgatgtggtc gccaccaacg aaaaactgca tgtgcgccag gtcaatatcg ttaaaaacgc
    64561 caccggatgt aatgccgaac aagcggaagc ggcgttaatt gcctgcgagc gcaactgtaa
    64621 aacggccatt gtgatggtgc tgaaaaatct cgatgccgca gaagctaaaa aacgcctgga
    64681 tcaacacggc ggctttattc gtcaggtttt agacaaggaa taacccatgg ccaaagagat
    64741 cagcagtgaa cttctgaaca ccattcttac ccgtgtcggc ggaccgggaa atatcgccag
    64801 ttgtggtaac tgtatgacgc gcctgcgtct gggtgtacat gacagttcac tggttgatcc
    64861 caatatcaaa acgctggaag gcgtgaaggg cgtcattttg accagcgacc aggtgcaggt
    64921 cgtttttggg cctggtaaag cgcatcgtgc cgcaaaagcg atgagcgagc tgctgggcga
    64981 tgcaccggta caggatgccg cagaaatcgc cgcccagaac aaacgtcagt taaaagccaa
    65041 acaaacctcc ggcgtgcaac aatttctcgc caaattcgcc accatcttca cgccgctgat
    65101 ccccggtttt attgccgccg gtctgctgct ggggatagcg acgttaattg ccacggtgat
    65161 gcacgttccg gcagatgctc agggcacgct acccgacgcg ctgaatttta tgaaggtgtt
    65221 cagcaaaggt ttgttcacct tcctagtgat tctggtgggc tataacgccg cccaggcatt
    65281 tggcggcacg ggcgtcaatg gcgcaattat tgccgcactc tttttgctcg gttataaccc
    65341 cgctgcaacc accggttact acgccggttt tcacgatttc tttggtctgc ccatcgatcc
    65401 gcgcggcaat attatcggcg tgctgattgc cgcgtgggcc tgcgcccgca ttgaaggcat
    65461 ggtgcgccgc tttatgccgg acgatctcga catgctgctg acctcgttaa tcaccctgct
    65521 gatcaccgct acgcttgcgt acctgatcat tatgccgctg ggtggctggt tattcgaagg
    65581 tatgtcatgg ctgtttatgc acctgaacag taatccgttc ggttgtgcgg ttttagccgg
    65641 gctgttcctg atcgccgtgg tatttggcgt gcatcagggc tttattcctg tttacctcgc
    65701 gttaatggac agccagggat tcaacagctt atttcccatc ctttcaatgg caggcgcggg
    65761 ccaggtgggc gcggcgctgg cactctactg gcgggcgcaa ccgcacagtg cgctacgcag
    65821 tcaggtacgc ggggcgatta ttcccggcct gctgggcgtt ggtgaaccgc tgatttacgg
    65881 tgtcaccctg ccccgcatga agccgtttgt taccgcctgt ttaggcggcg cggcgggcgg
    65941 tttgtttatc ggattgatag cctggtgggg tctgccgatg ggcttaaaca gcgcctttgg
    66001 gccgtctggt ctggtggcgc tgccgctgat gacttccgca caaggcatcc ttccggcaat
    66061 ggcggtttat gctggcggga ttctggtggc atgggtttgc gggtttattt tcaccacgct
    66121 ctttggctgc cgtaacgtca atctggactg atattatgaa acggacaatg ctctatcttt
    66181 ctctgctggc tgttagttgt agcgtcagcg ccgcaaaata ccctgttctg acagaaggta
    66241 gttgatcaag tatatgcggc gttaaagcag taataaaaag gccagtcagt aatgttactg
    66301 cctggccttt aatcaatgaa tcagaaacgc ttacagcgcc atcaacttgt ccagcgacgg
    66361 tgcgaaataa tagccgccgg ttaccggttt ggtgaaacgc aacatcgcat cacgtttgcc
    66421 atcggtatcg ccaaacatgc tcagcagttg ctgctcaatg ttatgcagac gcgcgcagta
    66481 ggcgcagaag tacagaccgt gagtgccact ggcggtgccg tacggcaggc tctggcgaac
    66541 aatcttcagc cctttgccat cttctttcag atcaacgcgg gtgaggtgag aggtttccgg
    66601 acgttcgtcg ccgtcaatct cttcgttggc ctctttggtg cgcccgatca tcatctcctg
    66661 atcgtgaacg ctcatccggt tgagctgctt caggttgtgc tcccaacgct gcacaaacac
    66721 atagctgccg ccggaatcca cgccgtcttt gataaccgcc acttcgcggc gcgtctcttc
    66781 acccgccggg ttttctgtac cgtcaacaaa gccgctcagg tcacgctctt ccacccaacg
    66841 gaagccgtgg atctcttctt tcacttcaat gcagtcacca aaggcttcca tcgccgcctg
    66901 agcgacagag aagtttacgt cgtgacgcag agaaagaatg tggatcaaca catcgaactg
    66961 ggtcgtcggc gcaaggcctt taccgtagcc cggaaaatct ttcagctctt ctgccccaac
    67021 gccgccgctc agagcgcgcc aggtgttgtt accaaaggca accaccgcac caagatgcgc
    67081 gtccgggaat ttcgcttcaa aagttgccag tttgtcggca aatgttttac tggccgcacg
    67141 cagggcgtca acttcccctt tcacgttggc ttcgatccaa atcgccgcgc ggcaatgttc
    67201 tggcaaaatg ccactctgaa cctgagacat tgttcctcct gaaaataaga atgccacgca
    67261 atccgtggca ttgttgagcg ctattttacg tgttttttcg cgacggcggt ttgttcagac
    67321 gcaaattaac gccgccagat aattttactg actttccagt ttttcagggt atcgtccgaa
    67381 ggcattaacc cttcaggacc acgccattcc ccgctgaact ggtagctaat atgttgactg
    67441 ccttcggctt tacattcgac ggcacgatta tcatcaccat cagctttttg gcaattgcca
    67501 aatgctttgc tgtaaaggtc gctaaacggt gtaccgattt taacaccggt gtcagcagga
    67561 atatcgctat ccagcacgtc aatgcgactg atcgtgcctt gatcgccgtt aatcaccatc
    67621 gccacgttgt cgcctttcat cacttcaaaa aagcgcacca cgttgccgtt cgtggttttc
    67681 attccgctgc gcaggcgata atcgccatca agcgcatcag caatggcttg ttcttgcagt
    67741 ggtgtggacg ccgttaattc acccacgccc tgctcgctca ctttggtgga cgatccaaac
    67801 cagttccacg ggttagcggc agaccagtta actgacgaca tcgtggaaca gccggtcagc
    67861 atcaacggca tagcgcataa cattaaacgc agcgatttca tgtcacttcc tttggttatt
    67921 caataacgtt gcttggagtg caaattcacc aaaaagtgcc gttattcttt ttcttcatta
    67981 aaacaagcgc gtaaacgtcg attggtcagc agccagatta acgccacaat atccgccact
    68041 accagcgcca gaccgatacc gctaacggat tcaccgttca gccacagcca cggttgccag
    68101 caaagtaaaa ccacctgcgc caacagcaac agaaaataga gcacatgcca ggtgcgagga
    68161 aacgaagccc gccgaccgct caacaaaaac gccagcaccg ccggaatacc gggaatcagc
    68221 cccagccaga aattatcgtg atcgggataa aacagattta gcagcgcagt accctgctca
    68281 cgcgacgcac cggcaatgac aaacagcacc caggttcgcg cctgaagcaa tagcacaagc
    68341 cagaagagca agggtaaacg caggcgtccg tgcgcatcat aatggacagg atggaactca
    68401 gtactcttca tcttcaatca aacgcttacc cagactcagc acgtcggcgt gttcatatcc
    68461 caggcgttca tacattccga gcaccatgtc gttatcttcc ggcacgttga tctgaatttt
    68521 cgggcagccg cgagcaatca gctttttctc cagccggtta agcaacgcat tggcaattcc
    68581 acgcccacga aactctggat gcacgccaag ataataagca gacccgcgat gcccgtcata
    68641 accgcccatc accgttccga ccacttcacc gtttacctca gcgaccaaaa acaaactgac
    68701 gtcatggttc atcttacgct cgatgtccat ttccggatcg ttccacggac gcagcaagtc
    68761 gcaacgctcc caaagggtga tgacctcttc gaaatcttcc tggcgaaata cgcgtatctc
    68821 catggtattc gttacctttt tgcgggttaa aaggctgatt atggcgtgaa cggtcgaatt
    68881 agccaatatc tgacgaaaat cggttgaaaa agtggcataa tggggagttg tcaactattg
    68941 aaatgaaaag taaaacaatt ctcaacagca aaccgtcgta acggattacg cgatacgata
    69001 taacatctgg aactttatta ttacaactca ggccgtatga gcacttttaa accactaaaa
    69061 acactcactt cgcgccgcca ggtgctgaaa gccggtttag ctgccctgac gttgtcagga
    69121 atgtcgcaag ccattgccaa agacgaacct ttaaaaacca gcaacggaca cagcaagccg
    69181 aaagccaaaa aatctggcgg caaacgtgtc gttgttctcg atccaggcca cggcggaatt
    69241 gataccggag cgatcggacg caacggttcg aaagaaaaac atgtggtgct ggcgattgct
    69301 aaaaacgtcc gttccatttt gcgtaatcat gggattgatg cgcgtttaac gcgttctggc
    69361 gatacgttta tcccacttta cgatcgcgtt gaaatcgccc ataaacatgg cgcagatctg
    69421 tttatgtcaa ttcatgccga tggctttacc aacccgaaag ctgccggtgc ttcggtattt
    69481 gccctctcta accgtggggc aagtagcgca atggcgaaat acctgtctga acgcgaaaac
    69541 cgcgccgatg aagttgccgg taaaaaggcg actgacaagg atcacctatt gcagcaagta
    69601 ctgtttgatc tggtgcaaac cgatactatt aaaaacagcc tgacgcttgg ctcgcatatt
    69661 ctgaaaaaga ttaagccggt gcataaactg cacagccgca acaccgaaca agccgcgttt
    69721 gtggtgttga aatcgccgtc ggttccttcg gtgctggtgg aaacctcgtt tatcaccaac
    69781 ccggaagaag agcggctgtt aggcacggcg gcgtttcgtc agaaaatcgc cacagcgatt
    69841 gctgaaggcg tgatcagtta tttccactgg ttcgacaacc agaaagcaca ttcgaaaaag
    69901 cgataagtta tgaaacccga cgcacaccag gttaaacagt ttctgctcaa ccttcaggat
    69961 acgatttgtc agcagctgac cgccgtcgat ggcgcagaat ttgtcgaaga tagttggcag
    70021 cgcgaagctg gcggcggcgg gcgcagtcgg gtgttgcgta atggtggtgt tttcgaacag
    70081 gcgggcgtca acttttctca cgtccacggt gaggcgatgc ctgcttccgc caccgctcat
    70141 cgcccggaac ttgccgggcg cagtttcgag gcgatgggcg tttcactggt agtgcatccg
    70201 cataacccgt atgttcccac cagccacgcg aatgtgcgtt tttttattgc cgaaaaaccg
    70261 ggtgcagagc ccgtttggtg gtttggcggc ggcttcgatt taaccccttt ctatggtttt
    70321 gaagaagacg ccattcactg gcaccgcacc gcccgtgacc tgtgccagcc atttggtgaa
    70381 gacgtttatc cccgttacaa aaagtggtgc gacgattact tctacctcaa acatcgcaac
    70441 gaacagcgcg gcattggcgg gctgttcttt gatgatctga acacgccaga tttcgaccac
    70501 tgttttgcct ttatgcaggc ggtaggcaaa ggctacaccg acgcttattt accaattgta
    70561 gagcgacgta aagcgatggc ctacggcgag cgcgagcgca attttcagct ctaccgtcgc
    70621 ggtcgttatg tcgagttcaa tctggtctgg gatcgcggca cgctgtttgg cctgcaaact
    70681 ggcgggcgca ccgagtcgat cctgatgtca atgccgccac tggtacgctg ggagtatgat
    70741 tatcagccaa aagatggcag cccagaagcg gcgttaagtg agtttattaa ggtcagggat
    70801 tgggtgtaac tccctcaccc ccattcccgc atccgctgat gcaacgtcaa cgacggcttc
    70861 tcggcaaaca gctgctggta atccgtggca aattgcccca gatgccagaa tccccactgc
    70921 atggcggcgt cttttaccgt tgtgctttgc gaccacgggc ttatcagttc gcggcgtacg
    70981 acgttcaggc gaatgcgttt cagccacgcg tttgggccaa tgcctaaaat agcgtgaaac
    71041 gcgttttgta gcgtgcggcg actgacatgc agttgattac acaaatccag caccgtcacc
    71101 ggttcggaca tgttttccag cacatattca cgggcgcggg aaagcaaccg acggtaactc
    71161 tgatgactga tgctttccgc cgtcaccatt ggttgcgctt cttccagcat cgcccccatc
    71221 gccattagca aattatcccc cagcactttt cgcactgcag gctgatggag attttccggg
    71281 ttctcgcaaa acgtcgccag cgcctgttgg acaaagcccc acagcgcggc tttatgctgc
    71341 tctttcactt ccagcgccga ctggctacgc agcatatgta atacccgatc cggattgtgc
    71401 aaaaagttag cctgacaggt gatgacatct tcagacagca ctacgcccag gatcgtataa
    71461 tcgtccggcg tacttaattc aaactcggtt ccaccggggc gcgtcgctat ttctgcgctt
    71521 cccagacatt gcgaaccgat aaatccctgc tcaccgcgcg tcgccggaat gccaaaccag
    71581 aacgagttcg gccagaccag gcacgactga cgcagcgcca gaccggtgta ttcacgaaaa
    71641 acctgaatat catcgagtag aatttccgta aattcaccat gaaacttgcc cggatgcagc
    71701 tgatcgtaaa tctgctgcta ggcggtaatc gtcaaagcat gttcatagac atccgttgtc
    71761 tgtcgttgat gaacattatc cacttcgacc ttcggcgtga gcttcaggtt ttcgggtaag
    71821 ggttcatgat aaagatggtg caaattggct gtacgggtct ttttcatgat gttaatgccg
    71881 ggtgttgtag gacacccggc acctccgaca ggttaatggg gcttgagacg ataacgacta
    71941 ctgcgtttac gtaacgtccc ggcagaaaag agctgttcga gcgcatttct ggctttttcc
    72001 agcggccagc caaagtgggc ggccacttct cctgccgtca ttccctgaca tactgatgtt
    72061 aacagcgcca acaactcgtc agcagattcc gcgacgataa ctggcgcgtc gggttccttt
    72121 acgggttgct tcggctggcg gttaaagcca gtaaccagcc actgggtgtc atcgtctggc
    72181 cgaccgatct ccttacggct gatgacacgc ccggtacgca tcgcggcagc acaacctgcg
    72241 tccagcgccg cacgacacgc cgccagatcg ccttccacca ccagcgttaa gcgaccaggg
    72301 tcaagcactt cgtgactgag cagacgaacg ttagctgctt tgagcatggc atccgctgca
    72361 tctatcgcgg cgaccatgcc gtccacttcc agcaatccca gtgcattgat catcggcaac
    72421 ctccgttacg cacgctggat tggattacgc gcgatttcca gcactgcatc ggtaaaggcg
    72481 ttacaggctg ctttacacgc ggcctggcta ccggttaaaa atgctgccga gtagttggtt
    72541 tcagacggtg gcgggacgta ggtcaccagc tgtacgtcgg cagatttcaa cgcggcatca
    72601 atgccgtagg tcgcttccag cggcggtgcc accagatacg ccatcggatc gccaagcgtg
    72661 atcccggcgg ttgatgaaag ataagaaccg gtacgcgaaa ccacatgcgc caggaaggcg
    72721 gtgttttctg cgtcattggc ccactggaaa gctgcgccat tttcaatatg tgcaaccatc
    72781 gcatccagac cggcacgcac ttccgccggg tttggcccac caagcataat cagcacttca
    72841 ccggcagtcg gtgacgggcc gtgtgccgct cccgcataca gcgagcgacc atataccact
    72901 tcgaccatcg cttgtttggt cgcttcgtca gccgcaatgt aggttacgtc gtcagaatcg
    72961 gcagaaatca gcccgaggct acgaatatgc ggcggcaatt ttagttcacg cgcaaattca
    73021 gcgttaacgg aggcaatcac ccgcatggcg gttaccgacg gtcgaatcaa atctaaagct
    73081 ggcatgatgc ctccttatcg ggtcatgttg atgccggatg ctttctgctc cagcatacgt
    73141 ttcgccaaat ccacaatgac ggcacagcag cacgaaagag aagcaaaagt cagctacctg
    73201 gtacactagg cagcgggtca gcaaaggggg gcaaatgccc cttttttcat gtcagataat
    73261 cagatcatta cctcaataaa ctaataatca catcgcggtt acaaccagtt tgatagtgat
    73321 agctcaaacc cagacgtggc gctggctgca agcaaaataa gccgcaaaaa tgcgacccca
    73381 tgtttactag ccgcaaaaat gcggtcctat ttgccagaac cttttattat tcaattggtt
    73441 agcgcaagaa aaagcgtgat tactgttcaa gaagtatcca gaaaggtcat gattttgacg
    73501 ctgaaaaaaa ataggtgggc atttttgccc actcactagc cgcaaatttg cccactcact
    73561 agccgcattt ttgcggccca aagtaataaa tacagagaaa taataaatac agatcagcgc
    73621 caagattttt cgtcttttcg gcggaatcat gagactagtg cttacgtcct gataacctcc
    73681 agagcagacc ttaacttctg tgcagcagga ctacgctcat tgccgctcgg ctaggtcaaa
    73741 tcctcacgat aaagctgttc ggattgtgac cataatgtta aaggactctt taaatcattc
    73801 cttgacgaat taactagctg gcttaagcgg aatagcatgc aggataaaaa caaaaccagc
    73861 ttagcagtgg ggagagcttc gcttcgctcg tctcctgggg ccaatgctca atgaaacgac
    73921 gtaaggcacg gagcgctcac cataggtaga cacataaaca tctgagagcg caccttctat
    73981 cgcgctacgc gctcaataaa aaccaacgac accgccacac tgcaagacca atggttttta
    74041 tcgcgtatac agaccagaca ggaaacagca gcaaagcagc gtatagccag gtgcagacag
    74101 cacactcaca cgacgcacag caggcttaag tgatcactca ggaaacttta tccccgccgc
    74161 cgatcaccgc gaactatcat gaccgcgcca aattcgaaag gaatggccca tagaacgcga
    74221 tgaacacaca taagcgctac acttacttaa cgtcgccaca gagcgattga gagcgcttaa
    74281 aatcgatgcg atttcattaa atttttctca ctcgcctgaa tggtggcgat caggactgat
    74341 cgcaacattg ccccgcctcg atattgagcg cataatagat acatacatta agcggaaaag
    74401 agataagttt attgtgaaaa tcagggggtt acatatcatg cgcatatcag aacaacaacg
    74461 gcagatcctc gactatctgg caaaaaacga atcaatcacc gcacgacaag ccgcagatct
    74521 cgtttatggt ggcaatgtta cccgcgtcca ggttgacgcc gcccgccgct cactcatgac
    74581 gatggttagt aatgggctaa tcaggaaaca aggccgcgct ttcgtagctg aaagacatat
    74641 caatcacgct tatagccagg acgttgaaga attgcgccgc cagatccttt gggtattcag
    74701 caaacagggt caggagtatt tcctttctga acacccaaat ttcgccgcca aagagttaac
    74761 agtaagcgga ttgtcgtgcg cactggatta tcttgtcggt gaaggttgcg ttacttacaa
    74821 gcagctatgg cacgccctga atacgcttgt agaagatggt ttactcattg ccacgcgtga
    74881 gaacatcgaa tactggataa tgccgcgttc aggtcgccgc aatcatgcac ggtatatttc
    74941 aggcaacaga tacagaacgc cggactttaa tccgacacag gaagatcggg accgtgccga
    75001 aatgatgtgc agggaacagg agaaaagaat tatggataag ttattttcga tgcagtgaac
    75061 acaagcgggc gcaatgcccg tttttattgc cttattccac gcctgacgaa tcaatttgat
    75121 cacacctgag aaaatgcata gccagaagtt aaggccgtta tattgcgatt gggagcgttt
    75181 taaacggtat caatttaatt aattctgatg tggtgatttg caggcaaaaa aaacccgccg
    75241 gagaagcggg aaatagagac atatcgttgt agttattaac agatcacttt gaggtcgagg
    75301 gcggctaacc ctcgctaata ttatcccgct attaccttgc gggtgcaaat aatcttttat
    75361 tgctttgatg atttaataaa tagcactttt tgctaacaaa aaccgctgtc aatatatggc
    75421 caaaaatgtc aacagaaaaa ataaggttta taatattaag tatcacatgg gtaatgggtt
    75481 cagggaatcg gaatccaccg cctcacggaa attcaaaatt tgcttcttct ttcctccgtg
    75541 ggcggcttct ttttcgtatc gaggtattta tgaaagcaat aacattagaa cagttcgcag
    75601 caatggcgaa tgtatcgacc agaacgctga ggcgctggct aaaaaaacca tcaaccggag
    75661 tccctttgcc atttacccca cgcggtactc gccaattatt ctgggagcac gaatgcaaag
    75721 aatggttgta cagaacagaa cgataataat cacgcatcac attttgaggt aaacatcatg
    75781 accaatatcg aaagattaga acgctgtgcc gatattatgc gacgcagatg gatttatgac
    75841 cctgaagcgg gtttattgtt tagccgtgaa acaaaagacg ttatcaaagg cagcatgacc
    75901 aataaagggc atttggttgt aacggtacat gaaggtaaat tcaaagtgac actcacctat
    75961 cagaaagcct gctatgtata cacatacggc gcttatgacg agacattata tgaagtgcac
    76021 cacgttaatt tgaacaaaca ggataaccgc atcaagaata ttcgcctgat gccgaaagaa
    76081 aagcaccgcc ggatacataa aaacgtccgc aaacttattc ggctgggcca cacacaatgg
    76141 cttcaaccgc aattaattga acaatcacta taaacacagg agtaaatacc atgcattcat
    76201 tagacagtta ttttcagcgc acgacagccc ctaaatcagc cgcccaggaa cgccgtgagg
    76261 aattccatga gaaagtgatg cgttctgctg attatattgc ggacaagttc gtggaaacgg
    76321 ttcgtccact tgttgatgaa gtagctgata aacttcagtc tgaaatgccg gaagatatgg
    76381 aaggcactgc aaagcggcga ctcatttgcg aattaagtcg tcgttttggc gtgtcaataa
    76441 gcgcttttaa ataatcgaaa aaagggggga aagataatgg acgcatttga ggtactggca
    76501 aataaaggcg ctgaactggt agcacagcgt gataaaaccg ccaacgaagg ggaacgcgca
    76561 ctgctaaata aacagattaa agcgatccgt atggcccagt ttaaattaat cagtaatgaa
    76621 gttatcgaaa caattaaacc gcagatccca caaatcgttg ctgacgctat taaagccgcc
    76681 ggactggaga aacgcattgc cgggctaaaa acgaacgcag agaaaatgga gatgtacaaa
    76741 cagagcggtg ctccgaatcc cgatgagtac ttctcaccgg atgttgagtt tatggcgcag
    76801 gttgaggaac gtctgggtag ctgtctcacc gaggaacagc gtcgctattt tgatggagtg
    76861 gatagttcgg caggtattga cctgaactcg tatttcggtc gtgaaattga gcattttgat
    76921 gccgccccgt ctatgccgga gccagaacca gaaatggcca ccgatcctga agctgagcaa
    76981 cggaaacgcg tattcaatgc gatagggatt cggtactgat cggataaaag aaagccggga
    77041 attaccccgg ctgacttaac aatgtgacca atgaacaaat cggacaagac aaacaggata
    77101 agcagtctcg aaacaatgtt cgggaactat attagcacaa gtccggagaa aatcatcatg
    77161 ttggcactac gcatcatgca gggcatcgct aagaccctcg cagaacatgt cttagacctg
    77221 aaacattcgc cactgagcaa acaggccatg aagcgccaaa cgcttcgtct gtgggctgaa
    77281 tattcgcttg gaactatcaa taaaatcatc gacatgaaat cggggccgag caaccagagc
    77341 gcggaggaaa tggagttcat caggcgttta attctcattc gcagagacat tcatagccag
    77401 ttacacagcg tgggaatcga tattaatgat gggacgggcg attaattttc ggacaagtac
    77461 tatgcgcgcg cgaggagctg aacatgaagc aagatattgc agacaggctg gagatactcg
    77521 aaggccagcg ggcagaagcg aaacaactac gtaagcaggc cagaagagcg cacaggaaca
    77581 acgaagcaga gttactaacc aaatacatca gtttcaccaa ttactgcatc tacgagtgtt
    77641 acaaggaaga tgcagaggac tggctggatt cattgccaga acagtactga gcagaataac
    77701 accacgtcat gttgttctga ggtgagcata tcacctctgt tcaggtggcc aaatttagtg
    77761 tgccacttca gcatcaccgt tgaaatcaca ataacgattt ttttgtaaat atgctctcac
    77821 atatgtagga atagaccagg ttggcattga aacacatcta ccatgaccat aataaagtaa
    77881 ttttttgata tgtttatggt tttatattaa ttaaaatata tctaaatttg atagttttaa
    77941 aattgcaaaa tccagattgt gtttgtaatg tttaatctga ttgtattgag tggcgtttat
    78001 tatgaaaaat aaatcaggta attcttgtta tagagaagca gtcgatattc atagctcact
    78061 tgagctttca tttatattgc ataaatatca tttgcgtgta gatgagaaga aatctcgaaa
    78121 tgtattcagc gccatacgtg atgagctcat ccttgatggg gattcacgac aaaacttagc
    78181 tactttctgt caaacatggc ttgatgatga aatccgtgag ttgcttaatt tagctattga
    78241 taaaaacatg atttataaag atgaatatcc acaaatggcg gaacctgaag gtcgctgtgt
    78301 gcgtatgctt tccgattcgt ggagtttccc cgattcgcga cacaccttag gttgctcaac
    78361 cattggttca tctgaagctg ctatgctcgg ccgcttggcg ttaaaatggc agtggtgtaa
    78421 aaaacgcgaa gtacaaggta aatcaaccga acctgatttg cgatcctgtg caaatatgct
    78481 ggcataaatt ctgccgttac tgtgatgtga aattgtgtga aattccactt gatgatcatc
    78541 acctgataat gaatgctaaa gaggttttga agcatgtcga tgaaaacact attggtgtcg
    78601 ttacgacttt aggtattact tatttttgta aagcagattg cgttagtgtt tttaagataa
    78661 gtgttaaagt taatattagt ttttatattt acatcgtaat tatatgtttc tattttaaaa
    78721 atgttcgaga gtataaaaat gtctaatgct aatatcgcac ccaaaagtaa gaaaatgaca
    78781 gtggggacac tcgctatcat gaatataact gctgtagtaa gtctacgatt tataccttct
    78841 gaagcagaat acggcttagg tgccattttt tactatacct ttgctgcaat tatgttttta
    78901 gtacctgttg cctttgttgc ggctgagcta gctacaacat atccacaaaa agggggggca
    78961 tttcgatgga taggagaagc atttggggct cgctggggtt ttgtaggaat gcttatgacc
    79021 tggctacaag tcatccccta ctttccaaca gtactgacat ttggtgcagt ctcggttgca
    79081 tttattgatg ttgatatggg ggtggcagaa tcaattgcca gtaataaatg gtatatttta
    79141 tttttcgtac tatttgttta ctggggagct atttgtgttg cattacgtgg cgttggtatt
    79201 ttctcgcagg tatctaagtg gtgcggtatt atagggacaa ttattccagc tattatcctt
    79261 gttattcttg gtttctccta tctgtggttc agcggtaaac cagcggtgat cccattaggt
    79321 tggggtgatc ttttccctaa tatagctaac ttccagaata ttgttcttgc cgccagcata
    79381 tttcttgctt atgcagggat ggaaatgaat gcggtccatg ttaacgattt agataatcca
    79441 actaaaaaat acccaattgc aattaccatc gcttctatag gtactattgc aatattcctg
    79501 ctcagtactt taggggtcgc ctttattatt cctacagata aaattagtct aactcaaagt
    79561 ttgttggttg cctacgatat gctgttcgaa tgggctggag tgccatggct tggctctgtg
    79621 atggcattta tgctagcaat tggcgtactg ggtggggtgg ttacttggat tgcagggcct
    79681 aatacaggtg tattagcgat cgcgaaagct ggttatttac ctaagttttt ccaaaaaacc
    79741 aatcgtcatg gaatggggca ccatctaatg tttgtacaag gtataattgt ctcagttctt
    79801 tctgttactt ttgtgataat gccttcagtt caagcagctt ttcagatttt atcacagtta
    79861 acagttatgc tctatttagt tatgtatatg ctcatgttcg ctagtgctat ctatctacga
    79921 cattctcaac ctgatacaaa acgcccatat cgagcccctg ctttattttt ctgggcaaca
    79981 cttggattct tagggtcttt tctggctttt gtattaagtt ttattccgcc cgagcaaatc
    80041 ccaactggta gtccaaccag ctatgttctt tatttgttag tattagttgc cctattttta
    80101 ttaattccaa tattaattta cgccttccga aaatctgact ggaaagatcc aaactctgat
    80161 ttcgaaccat tttcttggga aaaaagaact agtaattaaa ttttccataa gctacaaaag
    80221 atgaggttaa atatgtttag tgctgaatct ccaattggtg taatagactc aggtgtcggt
    80281 ggattgacat cggtaaagga aataattaat ttgcttcctg gtgaagatat tatctattgt
    80341 ggagataata tgaatgcacc ttatggcaac cgttctgccg atgatatcat cagtttgact
    80401 aaaaaaatgc tgactttttt acagtcaagg aatgtcaaac tagttgctgt cgcatgtaat
    80461 actatatcct caactttgga atccgaagaa tacagcggat atgctaagag ctttccgttt
    80521 cctatcttat ccatcattga acctgcagta gaagatgtga ttcgccagca atataaaaat
    80581 gttggtatta tcgctaccga gtttactatt aaaacggggt gtcacaaaga gctaataaaa
    80641 aaattgaatt caacaataaa tgtatttgga gaaccaagca aaaatcttgc aatgttaatt
    80701 gaggaaggta atcttaatgc tcctgcaatc ttgaatgaca tcaaaaaaca tgttaaccat
    80761 ttaatttccc tccatccagt aaatgaaatt attctcggct gtactcacta tccaattgtt
    80821 cagaattttt ttgaagcagt tgcaccggat ataaaattta ttaatcctgc acatgatcag
    80881 gctatctcta ttaaaaatca cttgggacag ctaaatttat taaactcctc aaatatagga
    80941 actttgcaaa taaatactag tggaagtatg gagatctaca aaacggtact aggggaattg
    81001 agcattacga aacctcatac attcagtatc aggcagtttt aaatttatcc ggtacaccgt
    81061 aaaatcccgt ccttctgaac tgcaccccaa aagttggaca ccaactgagt aaggtgcatt
    81121 ttatggacaa acccagttac atactgggta gtgcatccaa ttagtagaac atgtgttttt
    81181 cgataaacgc tccgatcact ttttcgtgga tctcaactga gcgtgagaag cagattgttt
    81241 tacgacccaa ccgcttaatg cgggtgcgta gcgtcagatt attacgctca atgcgttggg
    81301 tgaatatttt gccggtcaga tgcttattct tcggcacctc ccggccatag ctgccccagt
    81361 catcgctggt cagcatgccg atgttgaagg gtgtaagcag tgccagtagc tcccggcacg
    81421 tttgatcggt tcggggacca aaagtgtagg ccagtacacc gcctgttttg gtgttgtacg
    81481 cgtacccgag ccaatgttgc cgggctttac tgccaacgta gctccattgc tcatcaagct
    81541 cgcagataaa cgccacatca gcatgggcaa caggcgaaga agttattcgc tttggcgcga
    81601 gttttttaaa gtccggatga cggtgttact accaattttc agtgtcctgg cggtatcgcg
    81661 aaccccggca ccattaaagg ccatttcagt tatcagctct ttcatacccg gcttacgtgc
    81721 ttgataagta taagtgaact gaaacacacg gtggcagtca cggcagcgaa gtctgtcacg
    81781 gcctttaggg ttctgaccat ggcggtaaac ctgagctgac tgacaacggg gacaatgaat
    81841 attaacgctg gtcatgagat aacctcaaaa gcccgtatta tacatcagat tcaactaatt
    81901 agaggcatca cccatccagc attataaaac atactaacaa taactaaatt ataataatca
    81961 caacttacaa tcaaataata aatgcataag attaacaatg attttttcgg gttatattta
    82021 tctaaaaaag cagaacatta tgtaacttat tattttctaa gcgaaaattt attgtgcggt
    82081 cagactaaca aacttgcgta ccacccgaag aacatgcgaa gatttaagta ttctttcttt
    82141 tttggttttt atttttcatt ttctcttttc ttctctttcc ggttctgttc tggttttggt
    82201 tttttccttt ttatttttat tctttcttca gggttatgct aaaacaccaa aacatttttc
    82261 accacaaaag catccaatca tcttgccaga ccaatcacct taaccggata cactgccgga
    82321 tataatccca ctaacaccca gacaccaagc accatacctg ataactaccg tattcaggaa
    82381 ggaaatgcga gtgatttcac cgtcaccggc acaaccgatc cgccaaaaag aggcgtacca
    82441 atgtcgatat agtcccccgc gcggacaatc acttcgtcaa tgactgccaa cgggagttgt
    82501 tgtagctgtg ggcgcaacaa catgcccaga gctttaccaa agtcctgccc ggccaccacc
    82561 agcaggggat gcggattcgg aaaacgcgcg acgaaatcga ccagcgcgtt gatgaccgtc
    82621 agaaccgcgg cgtaacgcac aggcagcgag gcgggaagcg ccagcacgta cacgtcagtt
    82681 ttgggatcaa gatccagctg aagcagcgcc tgttgccagg cactcaccag atccgtttca
    82741 tcaatcggga tcgccaccgg caaattgcgc agcggcagtt gtacgccctc cagccagatt
    82801 gtgctgccag agagcgaaag ggtatgtgca cccgcgccaa tcaccgtgga gcgtacggtt
    82861 tgcgccggaa actgcacatt catctcacgc aggcgcggat ggtcatgcag cgccgttgcc
    82921 agaagtgggc caatatcggc aaaacagaac gggttggcgg gctggtggcg ataacattcg
    82981 cccacgcctc cagaaagcgt aatgatttcg ggcgtaacac ctgcgggcag caaaccggtt
    83041 tgcatcaatg cctgcgcgag cggcgaaagc gttccgtcaa tcacttcgac aatcagcgcc
    83101 gccatccgcc gggtaacctg caccagctgc gcgccggtca gcgaacgggc gtcagtgcct
    83161 gcaccgaagc actcatccac aatcatctgc cccggtttat gagcgtaaac cacgcgcccc
    83221 tggctgtcgg tttccagcag gcgaccaccg acgttgagac aggcagtgcc gctgattttt
    83281 ccggcatcga acagggcgta gttcgcggtg ccaccgccga tatcgatatt cagtacccga
    83341 cacagccgtt gttcagaaag ggtttgcgcc ccggctccgt gaccggcgat cacggattcg
    83401 aggtgcggcc cggcgctggc gacgacaaaa tcgcccagcg actgtttttc cttcatcact
    83461 ttttccatca gctgtgcgac caggctttcg gtaaactggc cttccggcag ctgggcaatg
    83521 atggtttcgc ggatgcgctg gctttcggtt ttgtcggctg gcacgctggc gggcacggac
    83581 gccgttaccg ggatcgattc atcacattcg gtgatggtaa aacccagtag atccgccact
    83641 tcacgggctt ccggggtaat aatgctggcg cgcagaacca cggacattgc ctgttcgccg
    83701 cgcgcgtgtg cttcacgaat atcattcgct gtgatgagtt ttttcacctg cttcctcctg
    83761 tttggtgaga gatgccagat aatccaccag ttgctgcacg ctttgcgggt cgtggctgtt
    83821 tagctcgaaa atcggctctt cgaaacccgt ctcaagcaat aactttcgcg ttgcggcgac
    83881 gtcagcatct ggcatgtccg ttttactgat gacggcgatt tgccgtttgc tgacgccaat
    83941 atccagcaac ccggcaggca accgactttc cgggtcattt gcgccatgga catagatcag
    84001 catgtctaca tcctgtagcg tggtaattaa ggcgtgatac cagcggggat ggctaaaata
    84061 ttcacccggc gtatcaatat cgcccttatc attaaattcc acggccagtg tttttctggc
    84121 gagggtataa ttcccctgca acgcattaaa taacgttgtt tttcctgccc cgacgctacc
    84181 cacaaacgca attcgtttca tgaatgcctc gtattaactt ttggtcattt cacaaagcgt
    84241 ataatttaat aagcgaccta aaccgctgac ggtttgtgat aacgcttctt ctactgcgcc
    84301 caccgagcca taaatcacca atgcaccgct gaagcggtcg agaaaaccga tatgcacatc
    84361 agcggctttc agcgctaaat caccggcgat catcgccgtt tcgccgggag ttagcgtcat
    84421 aatgccaatt gcgcccgcat ccggaacgcc gattttcttc gccagttcct caccgggatg
    84481 cgcgatgaga tgcgccagtg tgacctgttt gccgggcaca aattcctgaa tgatgcgttc
    84541 tttatccatg agtcgccgcc tgtgaaaaac tttcgtgtat cgtgacaatt tttcgtcaga
    84601 gtaaataaca aaattcggca agcgcgttta aaaggtgagg tagatcaata agaatggaga
    84661 gatatttact gttatgaata aactttcata tgcgacgcat gaatataaat aaaataatat
    84721 cgagatattt tatatactga tttttttatt attcgcaaac tcagtaataa gctttgctaa
    84781 tatatttcag gaaaaccgct taccctgaat attcagggta agcgtgagag ttaagaatga
    84841 attacagcgg ttgggtttgc gcttcaacca cggccagcgc caccatattg acgatacgac
    84901 gcaccgatgc gatcggcgtt aacacgtgaa ccggtttcgc cacgcccatc agcaccggac
    84961 cgacagtcac accttccgag ctggaaacgc gcagtaagtt gtaactaatg cgggcagctt
    85021 ccatgttcgg catcaccaga atattggcag aacctttcaa agggctgtcc ggcatacggt
    85081 cgttgcgaat cgcttccacc agcgctgcat cgccgtgcat ttcaccatca atcatcagat
    85141 ctggcgcacg atccctgacc agttccagcg cctgacgcat tttgctcgac gacgggcagt
    85201 cagaagaacc aaagttggag tgcgacaaca gagcaacgcg cggctcaata ccaaaacgac
    85261 ggacagtttc tgccgccatc aaggtgatct ccgccagctc ttctgcgtct ggttcgtcat
    85321 tgacgtaggt atcggcaata aaggtgttac cactcggcag cagcagcgca ttcattgcac
    85381 ctgcggtgtg aacgccatcg cgataaccaa agacattttt caccacacta aaatgttcat
    85441 gataatcgcc caccgtaccg cagatcattg catcggcttc cccacgctga accatgatcg
    85501 cgccgatcac tgtcgggtta ctgatcagcg cacgctgcgc ctgttcctga gtgacaccgc
    85561 gacgcttcat gatctggaag tattcggtcc agtactcttt aaagcgcgga tcggattcgt
    85621 tattgacgat ttcaaaatca acgcccgctt tgatctgcaa gcccagtttc tgaatgcgca
    85681 tttcgatcac gttcggacga ccgataagga tcggtttcgc cagtcccagc gttaccagtt
    85741 cctgagtcgc atgcagaacg cgcgcctctt ccccttccgg cagaactacg cgcttcggcg
    85801 ctttgcgagc ctgggagaaa atcggcttca taaacaggtt ggttttgtaa acgaactcag
    85861 tcagcttgtc gatgtagacg tcgaaatcag caatcggacg cgtcgccacg cccgactcca
    85921 tcgccgcttt tgcgaccgca ggagcgatct taacgatcaa gcgcggatca aacggttttg
    85981 gaatgatgta ttccggacca aagctcagat cctgaacgcc atacgctgaa gccaccactt
    86041 cactctgttc tgcatgggcc agttctgcaa tcgcgcgtac cgccgccagt ttcatctctt
    86101 cgttgatggc ggttgcgcca acgtccagcg cgccacggaa gatgaacggg aagcaaagaa
    86161 cgttgttcac ctggttcggg tagtcggaac ggccggtaca gatgatggca tccggacgca
    86221 cttctttcgc cagcggcggc agaatttccg gttccgggtt cgccagcgcc aggatcattg
    86281 gtgcccgagc cattttcttc accatttcct gggtcagcac tttcgggccg gaacagccca
    86341 ggaaaatatc cgcaccttca atcacatcat cgagggtacg tttgccgtca tccaccaccg
    86401 catatgcggc tttggtttcc gccatatttg gctcacggcc ctgatagata acgccttttg
    86461 aatcgcaaac cacgatgtta tgtttttgca gccccagcgc taccagcagg ttcatacagg
    86521 cgattgctgc ggcacccgcg ccggaaacca ccatccgcac gtcggagatg tttttctcca
    86581 ccacgcgcaa gccgttgagg atggcggcag tgctgatgat tgccgtgccg tgctgatcgt
    86641 cgtggaatac cggaatattc atccgctcgc gcagtttctg ttcaatatag aaacattctg
    86701 gcgctttaat gtcttcgagg ttgatgccgc cgaaggtcgg ttcgagcgcg gcgacgactt
    86761 caataaattt gtccgggtcg agttcatcaa cttcaatgtc aaatacatca atcccagcga
    86821 atttcttaaa tagaacgccc ttgccttcca tcaccggttt gcctgccagc gcgccaatgt
    86881 tgcctaaccc cagcaccgcc gtaccgttag agatcaccgc aaccaggtta ccgcgcgcgg
    86941 tatatttgta ggcttttagc gggtcttttt cgatttcaag acaaggtgcg gcaacgcctg
    87001 gtgagtaggc cagcgccaga tcgcgctgcg ttgccagagg cttggttgga gaaacctgga
    87061 ttttccctgg aactggaaat tcatggaaat caagtgcact ttgttttaac tggtcatcca
    87121 tttggttgtt cctttcacgt aacgttcaca aataaagtgt gtgggcaaca gcccctgccc
    87181 acaacgtggc gcacattatt accctgccgg agtctacaga ctttgagcaa gtccaaactc
    87241 tcaccattaa tataatgttt tggtaataat cctataacac tgatgttacc tgcttaatcc
    87301 agcaatacca tgcctgtctg ctatgctttt ttgatgcgtt tagcgaaatt tctcagaagt
    87361 gtgaattaac gcactcatct aacactttac ttttcaagga gtatttccta tgaacgagtt
    87421 agacggcatc aaacagttca ccactgtcgt ggcagacagc tgcgatattg agtccattcg
    87481 ccattatcat ccccaggatg ccaccactaa tccttcgctg ttactcaagg ctgccggatt
    87541 atcacaatat gagcatttaa tagacgatgc tatcgcctgg ggtaaaaaaa atggcaagac
    87601 ccaggaacaa caggtggtcg cagcgtgtga caaactggcg gtcaatttcg gtgctgaaat
    87661 tctcaaaatc gtacccggtt gcgtgtcaac agaagttgat gcacgcctct cttttgataa
    87721 agaaaagagt attgagaagg cgcgccatct ggtggacttg tatcagcaac aaggcgttga
    87781 gaaatcacgc attttgatca aactggcttc gacctgggaa ggaattcgcg cggccgaaga
    87841 gctggaaaaa gaaggtatta actgcaacct gacgctgctg ttttctttta cccaggcgcg
    87901 ggcctgtgcg gaagcaggcg tttttctgat ttcgccgttt gtagggcgta tttatgactg
    87961 gtatcaggca cgcaagccga tggacccgta tgtggtggaa gaagatccgg gcgttaaatc
    88021 ggtgcgcaat atctacgact actataagca acaccactat gaaaccattg tgatgggcgc
    88081 gagcttccgt cgcaccgaac aaatcctcgc cttaaccggc tgcgatcgac tgactatcgc
    88141 accgaatttg ctgaaggagc tgcaggaaaa agtttctcca gtggtacgta aattaatccc
    88201 gccttctcag acgttcccac gcccagcacc catgagcgaa gcggagttcc gttgggagca
    88261 caatcaggat gcgatggcgg tagaaaaact gtctgaaggc attcgtctgt tcgctgttga
    88321 tcaacgcaaa ctggaagatc ttcttgccgc caaactataa accagccacg gagtgttata
    88381 tgtcccgaaa agaccttgcc aatgcgattc gcgcactcag tatggatgct gtacaaaaag
    88441 ccaattctgg tcatcccggc gcgccgatgg gcatggctga tattgccgaa gtgctgtgga
    88501 acgattttct taaacataat cctaccgacc caacctggta tgatcgcgac cgctttattc
    88561 tttccaacgg tcacgcgtcg atgctgctct acagtttgct acatctgacc ggttacgacc
    88621 tgccgctgga agaactgaag aacttccgtc agttgcattc gaaaacccca ggccacccgg
    88681 agattggcta tacgccaggc gttgaaacca ccaccggccc gcttggacaa ggtttggcga
    88741 acgccgtcgg gctggcgata gcagagcgta cactggcggc gcagtttaac cagccagacc
    88801 atgagatcgt cgatcacttc acctatgtgt ttatgggcga cggctgcctg atggaaggta
    88861 tttcccacga agtctgttcg ctggcaggca cgctgggact aggcaagctg attggttttt
    88921 acgatcacaa cggtatttcc atcgacggtg aaacagaagg ctggtttacc gacgatacgg
    88981 caaaacgttt tgaagcctat cactggcatg tgatccatga aatcgacggt cacgatccgc
    89041 aggcggtgaa ggaagcgatc cttgaagcgc aaagcgtgaa agataagccg tcgctgatta
    89101 tctgccgtac ggtgattggc tttggttcgc cgaataaagc aggtaaggaa gaggcgcacg
    89161 gcgcaccact gggggaagaa gaagtggcgc tggcacggca aaaactgggc tggcaccatc
    89221 cgccatttga gatccctaaa gagatttatc acgcctggga tgcccgtgaa aaaggcgaaa
    89281 aagcgcagca gagctggaat gagaagtttg ccgcctataa aaaggctcat ccgcaactgg
    89341 cagaagagtt tccccgacgg atgagcggtg gtttaccgaa ggactgggag aaaacgactc
    89401 agaaatatat caatgagtta caggcaaatc cggcgaaaat cgctacccgt aaggcttcgc
    89461 aaaatacgct taacgcttac gggccgatgc tgcctgagtt gctcggcggt tcggcggatc
    89521 tggctcccag caacctgacc atctggaaag gttctgtttc gctgaaggaa gatccggcgg
    89581 gcaactacat tcactacggg gtgcgtgaat ttggcatgac cgctatcgcc aacggcatcg
    89641 cgcaccacgg cggctttgtg ccgtataccg cgacgttcct gatgtttgtt gaatacgccc
    89701 gtaacgccgc gcggatggcg gcactgatga aagcgcggca gattatggtt tatacccacg
    89761 actcaattgg cctgggcgaa gatggtccga cgcaccaggc tgttgagcaa ctggccagcc
    89821 tgcgcttaac gccaaatttc agcacctggc gaccgtgcga tcagatggaa gcggcggtgg
    89881 gctggaagct ggcggttgag cgccacaacg gaccgacggc actgatcctc tcaaggcaga
    89941 atctggccca ggtggaacgt acgccggatc aggttaaaga gattgctcgt ggcggttatg
    90001 tgctgaaaga cagcggcggt aagccagata ttattctgat tgccaccggt tcagagatgg
    90061 aaattaccct gcaagcggca gagaaattag caggagaagg tcgcaatgta cgcgtagttt
    90121 ccctgccctc gaccgatatt ttcgacgccc aggatgagga atatcgggag tcggtgttgc
    90181 cttctaacgt tgcggctcgc gtggcggtgg aagcaggtat tgccgattac tggtacaagt
    90241 atgttggtct gaaaggggca attgtcggga tgacgggtta cggggaatct gctccggcgg
    90301 ataagctgtt cccgttcttt ggctttaccg ccgagaatat tgtggcaaaa gcgcataagg
    90361 tgctgggagt gaaaggtgcc tgatggtgat cgccggatgc tgtatgttcc cctcacccta
    90421 accctctccc cagaagggcg aggggacggt ccgtgcgctt tttagggccg catctgcgat
    90481 tttgtttaac gggtgatcca caacgtgggc ctggcgtctg gcccatgcca gttatcgcag
    90541 gtaggttctg cggcgtaacg caccaggcga aaacgctgac cgtcaaagcg ccagcgcgcc
    90601 tgaatgccac aatcgcttaa tccgcgccct ttcgctaagg tcaccagttc acgcgatttc
    90661 tcatcaaatg ttgcgttcat cagttccagt tcattcgtct cctggccgtt gttgaacggc
    90721 aaacgcaacc gaaccgggcg cgaggctagc ggctttttac gcgacacaat ccatgccaaa
    90781 tcaatggtgt tataggcccc tgcctcacag ctaatcatca tcagtgcttt atcatcggtc
    90841 agcgcagtga cattcacctc acgacgtaac ggatcaagcg agcagcgcag accattcatc
    90901 cgccagtttc cataatccag caaatcgtta cgttcttcga gtgagagtgg cgtcggcgtt
    90961 gggttaacca ccgcgacctc tttcagcgca ggcgcgggcg gtacgctgag aggcggttcg
    91021 tcccctttct tgatccacgc ggtttcactg ccaacgcgct tttgctgagc atcaataaac
    91081 aacaacgccg cttttaagcc gctcagagaa atggtctgat tgccgtcgcg taaggtgatc
    91141 gccttccctt cctgaattat ctgcaaaaac gcggtgatgg ttgccgtatc atcggttact
    91201 aataaccatg gtgaaatccg ccacttgtca ccacttaatg ccaacggctc accatctaat
    91261 agcagccgtg gcgctatctc cccttcagac gcatccggcg acttcaaccc accgcgctca
    91321 atacgtaaaa cggcatcggt atgcgcccca gcgctgcggc tcagggtcat caccagtcca
    91381 ttatgatcgc ccgtattgcg cgccacacaa aaattttgat tattacaggt gacctgccag
    91441 tcggaaaacg cccgttgcgc tggtgccgcc cacaccaaag acgtcggcaa caaagcgaaa
    91501 aaaaagagaa ggaaaatgcg atagcgcatg gacggtacga ccccagaagt tcaaacaaac
    91561 agcacaagtc gtatcttcgt tgcgcaggta gcgcggctca atcggatttg tcggattgag
    91621 ccaataataa acaacgaaat attattgcta tttttcagtc cattaaatgt gacatttgca
    91681 aatagttaag caataacacc gtcttaccgt cacgtatctc gccggttttg atcatctcca
    91741 gcgcctggct gaacggcagc tcgagcactt caatatcttc atcttcaaca ccgccaccgg
    91801 cgttagcgcg ctgattgtca ctgtattcgg cgataaaaaa gtggattagc tcagtcacac
    91861 cgccaggcga catatacagt tcaaataatt tgcgcacttc gcccacttca tagcccgtct
    91921 cttcaatcgc ttctttacga atgcacactt ccggttcgtc gttatccagc agcccggcgc
    91981 aggtttcaat cagctgcccg ctttcattgc cattaaccca ggtagcgaca cggaactgac
    92041 gaatcagaac cacgctcttt ttcttcgcgt tgtacaggag gatcgtcgca ccattaccgc
    92101 gatcgtaaac ttcacgttta tggcggataa cttcgccatc tttgcgcgtg agatcgtaag
    92161 taatgttgtg cagggtgaaa tagttatcgg agagaatttt gtctttaatg agggtgattt
    92221 gttgcgtcat accgactcca cagcgcgaaa tgaacaatta tattacgctg tgaagtcggg
    92281 tttgtctgcg caggctatca gtgagatttg actgatttta cccccagcca gtcaataatc
    92341 ccctgtgccg catgacgtcc ttctgccatt gcggtaacca ccagatccgc accacgcacg
    92401 gcgtcgccac cggcgaagat tttcggattg gtggtctggt aacggtactg gctttccaca
    92461 tccgcgatga tgcggcccca tttgtctacc gttacaccgt gcgactccag ccacggcatc
    92521 ccgtgtggat tgaagccaaa cgccataatc accgcatcgg ctggcatgac aaattcactg
    92581 ccctcaatcg gtacaggtcg ccgacgcccc tgggcatccg gctcgccaag acgcgtgcgc
    92641 aggaaacgaa ttccgcaaac gtgaccttgc tcattcagct caagcgccac cggctggacg
    92701 ttaaattcga agttggcccc ctcttcgcgg gcgttcttca cctctttctt cgagcctggc
    92761 atgttagctt catcacgacg ataagcgcag gtgacgttac tcgcgccgtg gcgcagcgca
    92821 gtacgcacac agtccatcgc ggtgtcgccg ccgcccagta ccacgacgtt aagtccggcg
    92881 gtattgataa acggctcttc cggtagctct tcgagcccca tcacctgttt agtgttggca
    92941 atgaggaacg gcagcgcgtc ataaacgccc ggcgcatctt cattgggtaa acccgctttc
    93001 atggaacggt aagtgcctac gccaacgaag accgcgtcgt attgttccaa aagcaaatcc
    93061 aaagagacat ctttacccac ttcacaattg agttcgaagt gaatccccat cgcgctgaag
    93121 atttcccgac ggcgtgccag cagggattta tccagtttga aagaaggaat gccgaaagtg
    93181 agcaagccac cgatttctgg atggcgatcg tacaccgtca ccgccacgcc attgcgggtc
    93241 agaacatccg cacaggccag ccctgccgga cctgccccga taatcgccac ccgcttgtcc
    93301 actttggtga catgacttaa gtcaggacgc caacctttcg ccaacgcctg atctgaaata
    93361 tagcgttcaa tgttgccgat agttaccgcg ccgtgctcat cgcgaatagt acaggcacct
    93421 tcacacaaac ggtcttgcgg acaaacgcgt ccggtaattt ccggcagggt gttggtctgg
    93481 tgagaaagct cgacggcggc gtcgatgttt ccggctttca ccagttcaat ccactgcgga
    93541 atatggttat gcagcgggca ggtccattca caaacgctgt gctcgccgaa cttaaggcag
    93601 cgcgaggctt cccgttgtgc ctggtcggcg cgaaatggca gataaatttc atcaaaaccg
    93661 gttttgcgcg cttcaatcgc cagtttatcc ggctcgccac gcgcgggcgt tgcctgcatt
    93721 tgttcgactt tactcattac cggcatttct tgtgcagcgg tactggcatg ccacggttga
    93781 tgctcctgac gtgcggtgcg caagcggcgg gatttcgcca tgccggagag tgcgacgtca
    93841 gtgaccagtt gcagcgcgtc cgccgggcaa ttctcgacac aggcaggacc gttttcgcgc
    93901 cctgcacaaa ggtcgcattt atgcgccgtg gcttttactt ttcctgccgc gacgggtgtc
    93961 aggacgattt gcatcgtacc aaacggacag gccaccacgc aggatttaca gccaatacac
    94021 ttttgctgat tgacctgaat gctgtcatca acgtggctga ttgcgccatt agggcagcta
    94081 cgggcgcagg gcgcatcttc acaatggtga caggtcactg cactacgttg ctgctgatgt
    94141 ttgataaccg taattcgggg atgaaaatgg tgttggctca ggacatgttg ctcatcattg
    94201 tgagccatga cacaggcgat ttcacaagca tgacaaccca gacattgctg actgttggcc
    94261 ataataaaac gattcataac gaccttcttt tttggttgta aaaaccttat tctttatatg
    94321 agtgttgtta ttacccgact tacaggggaa ccggcaatgt tcatgtgccc agaataagta
    94381 actatttcgc tataaactgt ggcagatcaa ataatcccat cactgactaa attgcgtttc
    94441 agcgaactgg aacattaatg attttttgtg gagaagacgc gtgtgattgt taaacgaccc
    94501 gtctcggcca gtctggcccg ggcctttttt tacattgtgc tgctgtcgat tctttccacg
    94561 ggtatcgctc tgctaactct ggcgagcagt ttgcgcgacg ctgaggctat caatattgcc
    94621 ggatcgctgc gtatgcagag ttaccgcctg ggctacgact tgcaaagtgg cagtccacaa
    94681 ctcaatgcac atcgccagtt gtttcagcag gcactgcatt caccggtatt aaccaacctc
    94741 aacgtctggt atgtgccaga agcagtaaaa actcgctatg cgcatctgaa tgccaactgg
    94801 ctggagatga ataatcggct cagcaagggc gatttgccgt ggtatcaggc caatattaat
    94861 aattatgtta atcagataga cctgttcgtg ctggctttac agcactacgc tgaacgcaaa
    94921 atgctgctgg tggtggcgat ttccctggct ggcggtatcg gtattttcac gctggtcttt
    94981 tttactttgc gccgcatacg ccatcaggtg gttgccccgc tgaatcagct ggttaccgcc
    95041 agtcagcgta ttgaacacgg gcagttcgac tcgccgccgc tggataccag tctgccgaat
    95101 gagcttggtc tgcttgcaaa aacctttaac caaatgtcga gcgagctgca taaattgtac
    95161 ctttcgctgg aggcgtcagt agaagaaaag acccgcgatc tccacgagac caagcgtcgt
    95221 ctggaggtgt tgtatcagtg cagtgttccc aggcgctgaa caccagccag attgatgtgc
    95281 attgtttccg ccatattttg cagattgttc gcgacaatga agcagctgaa tatctggagc
    95341 tgaacgttgg ggataactgg cggattagcg aagggaagcc aaaccctgag ctgccaatgc
    95401 agattttacc ggtgacaatg caagagacgg tttacggcga actgcactgg caaaatagtc
    95461 acgtttcatc atcagaaccg ctgcttaaca gtgtttcatc gatgctggga cgcggtttgt
    95521 actttaatca ggcgcagaag cattttcagc agttattgtt gatggaagaa cgtgcgacca
    95581 tcgcccgcga attgcacgac tcgctggctc aggtgctttc ttacttacgt atccagttga
    95641 cgttactgaa gcgttcgata ccggaagata acgccaccgc acaaagtatc atggccgatt
    95701 tttcccaggc gttgaatgat gcttatcggc agttacgcga gctgttgact actttccgcc
    95761 tgacgctgca gcaggcggat ctcccctccg cgttgaggga aatgctggat acgttacaaa
    95821 atcaaaccag cgctaaactg accctcgact gccgtctgcc aaccctggca ctggatgcgc
    95881 aaatgcaggt gcatttgttg caaattattc gcgaagcggt gctgaatgcg atgaagcacg
    95941 ccaacgccag cgaaatcgcc gtcagttgcg tcaccgcgcc ggacggcaat cacacggttt
    96001 atatccgtga taacgggatt ggtatcggtg aaccgaaaga acccgaaggt cattatggac
    96061 tgaatatcat gcgcgaacgc gcggaacggc tgggcgggac gctgactttt tcacaacctt
    96121 ccggcggcgg cacgttagtg agtattagct ttcgctccgc agagggtgag gggaagggga
    96181 acacgccggc tgaggtgtaa ataattaccc tcacaccggc cctctccctt taaaggcgag
    96241 agggaaaaac gtgcccatat tgacctgtaa gcgagtgtgt cagcaatctc atgataattt
    96301 acattaactc ctttttttct ccacgattgg ctcgtacctt gccgctacag tgaagcaagt
    96361 caagcctaca acaatacgca gaaacacgag gtcctctttt aatggcgaat ttctttattg
    96421 atcgccccat ttttgcctgg gtgctgacaa tcctgttgtg tctgacaggt accctggcga
    96481 ttttttcatt gcccgttgaa caataccccg atctcgcgcc accgaatgtg cgagtgaccg
    96541 ctaactatcc cggcgcatcg gcccagacgc tggaaaacac cgtgacccag gttatcgagc
    96601 aaaatatgac cggcctcgat aatctgatgt atatgtcatc tcagagcagt ggcaccggtc
    96661 aggcatctgt cactttaagt tttaaagcag gcaccgatcc ggacgaagcc gtgcagcaag
    96721 tacaaaacca gctgcaatca gccatgcgaa agttaccgca ggcggtgcaa aatcagggcg
    96781 tgacggtgcg taaaaccggc gataccaaca ttctgaccat tgccttcgtc tctaccgatg
    96841 gctcgatgga taaacaggat attgctgatt atgttgccag taatattcag gacccgttaa
    96901 gccgcgtgaa tggcgtcggg gatatcgatg cctatggttc gcaatattcc atgcgtatct
    96961 ggctggaccc ggcgaaactc aacagtttcc agatgacggc taaagatgtc actgatgcca
    97021 ttgagtcaca gaacgcgcag attgcggttg ggcaacttgg tggtacacct tccgtcgata
    97081 agcaggcgct caacgccacc attaacgccc agtcactgct gcaaacacca gaacagttcc
    97141 gcgatatcac cttgcgggtg aatcaggacg gctcagaggt aaggctgggc gatgtcgcca
    97201 ccgtcgaaat gggggcggag aaatacgatt atcttagccg cttcaatggt aagccagcct
    97261 ccgggttggg ggtaaaactg gcctccagcg caaacgaaat ggcgacagcg gagctggtgc
    97321 tcaatcgtct cgacgagctg gcgcagtatt tcccgcacgg actggaatac aaggtggcgt
    97381 atgaaaccac ctcgtttgtt aaagcctcca ttgaagacgt ggtgaaaacg ctgctggaag
    97441 ctatcgctct ggttttcctc gttatgtatc tgttcctgca aaacttccgc gccacgctga
    97501 tacccactat cgccgtgccg gtggtgttga tgggaacctt ctccgtactt tacgccttcg
    97561 gttacagcgt caacacctta accatgttcg cgatggtgct ggcgatcggt ctgctggtgg
    97621 atgacgccat cgtggtggtg gaaaacgtcg aacgtattat gagtgaggaa ggactcactc
    97681 ctcgcgaagc cacacgtaaa tcgatggggc agatccaggg ggcactggtc gggattgcga
    97741 tggtgctttc ggcggtattt gtaccaatgg ccttcttcgg cggcaccacc ggtgccatct
    97801 atcgccagtt ctctattacc attgttgcgg cgatggtgct gtcagtactg gtagcgatgc
    97861 tcctcactcc ggctctgtgt gccacgctac ttaagccact gaaaaaaggt gagcatcacg
    97921 ggcaaaaagg tttttttgcc tggtttaacc agatgtttaa ccgcaacgcc gaacgctacg
    97981 aaaaaggggt ggcgaaaatt ctccaccgta gcctgcgctg gattgtgatt tatgtcctgc
    98041 tgcttggcgg catggtgttc ctgttcctgc gtttgccgac gtcgttctta ccgctggaag
    98101 accggggcat gtttactacc tcggtacaat tgcccagcgg ttcaacccaa caacagaccc
    98161 tgaaagtcgt tgagcaaatc gagaaatatt acttcaccca tgaaaaagac aacatcatgt
    98221 cggtgtttgc caccgttggt tctggccctg ggggtaacgg gcaaaacgtg gcgagaatgt
    98281 ttatccgcct gaaagactgg agcgaacgcg acagtaagac cggcacctcg tttgccatta
    98341 tcgagcgtgc aacgaaggcg tttaaccaaa ttaaagaagc tcgcgttatc gccagcagcc
    98401 caccagcaat tagcggtctt ggtagttctg caggttttga tatggagttg caggaccacg
    98461 ctggagcggg tcacgatgcg ctgatggcag cacgtaatca gttgctggcg ctggcggcgg
    98521 aaaacccgga gctaacccgt gtgcgccata acggcctcga tgacagtccg cagttgcaga
    98581 ttgatatcga ccagcgtaaa gctcaggcgc tgggcgttga tatcgacgat attaacgaca
    98641 cactgcaaac cgcctggggt tcgagctatg tgaatgactt tatggatcgc ggtcgcgtga
    98701 agaaagtcta tgtgcaggca gctgcgccgt atcgcatgct gccagatgac atcaatctct
    98761 ggtatgtccg aaataaagat ggcggcatgg tgcccttctc tgctttcgcg acctcacgct
    98821 gggaaacagg ctcgccgcgt ctggaacgct ataacggtta ttctgcggtt gagattgttg
    98881 gggaagccgc accgggggtc agtaccggta cggcgatgga tattatggaa tcgttagtga
    98941 agcagctgcc aaacggcttt ggtctggagt ggacggcgat gtcgtatcag gagcggcttt
    99001 ccggcgcgca ggctccggcg ctgtacgcca tttccttgct ggtggtattc ctgtgtctgg
    99061 cggcattgta tgaaagctgg tcggtaccgt tctcggtaat gctggtcgtg ccgctggggg
    99121 taatcggcgc gctgctggca acctggatgc gcgggctgga aaacgacgtt tacttccagg
    99181 tgggcctgtt aacggtcatt ggtttatcgg cgaaaaacgc catcctgatc gtcgagtttg
    99241 ctaacgagat gaaccaaaaa ggccatgacc tgtttgaagc gacgctccac gcctgccgtc
    99301 agcgtttacg cccgattctg atgacctcgc tggcatttat cttcggcgta ttgccaatgg
    99361 caaccagcac gggtgccggt tccggtggtc agcatgcggt gggtactggc gtaatgggcg
    99421 ggatgatttc agctactatt ctggctattt acttcgtgcc gctgttcttt gtgctggtgc
    99481 gccgccgctt cccgctgaag ccgcgcccgg aataagcaat aaaaaaggcg acatgccaat
    99541 gtgtcgcctt tttcaacttt ccgattaaga acctgctcag cgggttcttg ctgtttatac
    99601 tttgtctcag gaattactta cgaagcataa cttcgataaa gtctttccag ttccccagtt
    99661 cacgttcaat cataacaacc tctcttataa ttatgggtat tctacggaaa caatataccg
    99721 tggtgaagct aatttactcg attgctgcga tgactacctc cgggggacaa accttatgta
    99781 aatactatgg tcctgcggtg ataatttgta tgtgatacac agcaacattt cgagatattc
    99841 atacggcatc taatactgat ttaattctgg ttaaaataca gacagataac aagatgaata
    99901 ttcttaatgt ttacgttaaa aatgtttaat attatttaat agttgttaat ttgaatactt
    99961 cgataatgtt atattttctg ataatcattt gcaggcaaaa tgttttcacc cttaaatgag
   100021 tatttattct cataaatcga aaaaggattc attatggtta cactttacgg catcaaaaat
   100081 tgtgacacca ttaaaaaggc tcgccgttgg ctggaagcca ataacatcga ctatcgtttt
   100141 catgattacc gcgtcgatgg gctggacagc gaattattga acggttttat caacgaatta
   100201 ggctgggaag cgttactcaa cacccgtggt acaacctggc gtaaactgga cgaaactacc
   100261 cgcaataaaa tcaccgatgc ggcctctgcg gcggcattaa tgactgaaat gcctgcaatt
   100321 atcaaacgtc cattgctctg cgcgcctggt aagcctatgc tgctgggttt cagtgattcc
   100381 agttatcagc aatttttcca tgaggtgtag tctatgtcgt gcccggttat tgagctgaca
   100441 caacagctta ttcgccgccc ttccctgagt cctgatgacg caggatgtca ggctttgttg
   100501 attgaacgtt tgcaggcgat cggttttacc gttgaacgca tggattttgc cgatacgcag
   100561 aatttttggg catggcgtgg gcaaggtgaa acgttagcct ttgccgggca taccgacgtg
   100621 gtgccgcctg gcgacgccga tcgttggatc aatccgccgt ttgaacccac cattcgtgac
   100681 ggcatgttat tcgggcgcgg tgcggcagat atgaaaggct cgctggcggc gatggtggta
   100741 gcagcagaac gttttgtcgc acaacatccc aaccatacgg ggcgactggc atttctgatc
   100801 acttctgatg aagaagccag tgcccacaac ggtacggtaa aagtcgtcga ggcgttaatg
   100861 gcacgtaatg agcgtctcga ttactgcctg gtcggcgaac cgtcgagtat cgaagtggta
   100921 ggtgatgtgg tgaaaaatgg tcgtcgcgga tcgttaacct gcaacctcac cattcatggc
   100981 gttcaggggc atgttgccta cccacatctg gctgacaatc cggtacatcg cgcagcacct
   101041 ttccttaatg aattagtggc tattgagtgg gatcagggca atgaattctt cccggcgacc
   101101 agtatgcaga ttgccaatat tcaggcggga acgggcagta acaatgttat tccgggtgaa
   101161 ctgtttgtgc agtttaactt ccgcttcagc accgaactga ctgatgagat gatcaaagcg
   101221 caggtacttg ccctgcttga aaaacatcaa ctgcgctata cggtggattg gtggctttcc
   101281 gggcagccat ttttgaccgc gcgcggtaaa ctggtggatg cggtcgttaa cgcggttgag
   101341 cactataatg aaattaaacc gcagctactg accacaggcg gaacgtccga cgggcgcttt
   101401 attgctcgca tgggggcgca ggtggtggaa ctcgggccgg tcaatgccac tattcataaa
   101461 attaatgaat gtgtgaacgc tgccgacctg cagctacttg cccgtatgta tcaacgtatc
   101521 atggaacagc tcgtcgcgtg atgagtggtt ctgcaagagg aaataagcat ggactggctg
   101581 gctaaatatt ggtggattct ggtgattgtc tttttggtag gcgtcctgct gaacgtgatt
   101641 aaagatctca agcgcgtcga ccataagaaa tttctcgcca acaagccgga gcttcccccg
   101701 catcgtgatt tcaacgataa gtgggacgat gacgacgact ggccgaaaaa ggatcaaccg
   101761 aagaagtaag ctgagtgctc aaccgtaggc cggataaggc gttaacgccg catccggcaa
   101821 tggtgaacga tccctgatgc gacgctcacg cgtcttatca ggccaggtaa tctgtctttt
   101881 acatcatttc aatcacatca tcatcgccag gcttaccgcc acttagcgct tggtcgaaat
   101941 aatgtttcgg aatggtataa cgcaagtgat ccagggcaaa ttgcatactg cgattgtcga
   102001 tcgcatggcc taaatcctcc acgatatcca gcgtcacgtc accaccggca ctgattaacg
   102061 cttcctgcgc cgcaactgca tgcgccagat cgataaccgg atcttcacca ccatgaatca
   102121 agtgaattgt cgtggctgta gacgcggtct ccggcaggct cgcatagcgt ccgttaaagg
   102181 caatgacgcg cgaagcaagg cccggttcgg ctttaatgct ctccagcgcc ataatcgcgc
   102241 cttgtgagaa accaattagc gccgtggcgt tagcccctac tccgctctgt ttctgccagt
   102301 agcgtaccgt ttcaataaac gtcggcatga tggcatccac acgcgcctgg cgattatctt
   102361 ccgtaatacc ctgcactgaa aaccactgac gccccgccgg attaccgctc ggctccgcgc
   102421 caccgacact caccaccagc gcatcaggaa acagcggtgc aaaccagctg cctatttccc
   102481 ccattgccac cgggttatcc ccgacaccgt ggaaaagcag taacaactgt tgtgcaggtt
   102541 tatccgggct ttgaacaaca aaatggtcat gtttcatggc gaactcctta actgatgtca
   102601 ttaattttac gccgctgact cacaatgacc atgggagaaa attgaaggag tcagtgaaaa
   102661 aattgccatt gcgttatgcg atcgcgcaga cgctcagtgc gaacctcatt tagtgcgaac
   102721 agcgcctgcg cggcctcttc ccgctggcga accagtaaca tcttgcggcc tgaaagttta
   102781 agtgtggtac ataactgcgc gtcactggcg tttttctgta aacgcccacg cagcgccggt
   102841 aatgccagtt cactggtttg tagcagacgc aataagcaac ctaacgatgt taatagcgga
   102901 cgatgagcga aagcaaaacc ggccagttca agccagtcgt cgtcagaaag gatggcatcg
   102961 tttagtggat caacaggaag catttcgcca ttccactgcg cgagagcttg cgcatcgcga
   103021 cgtaaacggt aatgctcacg ttcagccagc tgtttacccg catcactcat cggtaacagc
   103081 gccatcgccg tatagcaacc gctgctggct tcccgatgat tacccatccg caccagcaca
   103141 aaaccgcagc gttgccagaa acgccataac tccccagtgt aaccaaaact caccgaaaga
   103201 tagtcgaggt catgcgtata ttgcaaagct ccagcaataa gttgtcgccc tgtgccttcc
   103261 cgctgacgag ccggatgaac tgctatccgg ctaacccgcc ttccgcgcaa tgtcgccgcc
   103321 agtggattgc tgccgtgcgc cgccagcgac tgggccacca gattaccccg cgggcgacga
   103381 aaacctgccc ataccgcctg actgagttct tgagataatc cgccctcatc caccagccac
   103441 aacgccccgg caatctcgtt ttcgccagcc gcctgtaaaa aatgttgccc tggtgcatcc
   103501 atcatccggc gtaaatccag cggcgaggtc cggtagtgcg caccagacaa taactgataa
   103561 acctttaacg gcgtttctgg ctcgcttcgc cataacgttt gttcaaatgc ggaaatgacg
   103621 atattgcctt gtggtgtatg ggtgaagttt tcatcgttaa aaaccagtgc ctcactaacc
   103681 attttttcca gcgggcatcc ctgtgcccag cggatcggct gttgcagttc aaaacggtgt
   103741 aaatgcggaa agcgagcgca aaatttcagc aaaaaaccac gtccggtacc ttcgtagccc
   103801 tgcaccgtag tggttaacaa cgttcgagga aaacgcgata ccagttgatg caacaacggc
   103861 gcaggtatgg ctgcggcttc gtcgaccacc agccagtcgg cttgctcatc gctggctaac
   103921 aaggcatccg gtgcaataaa gcgaaacttc tcgcccgcaa attgtgccag tacatccgtt
   103981 gccgcttttg cgggcgcggt gacaatcgca ctgcccgcaa tacgagaaat gagttgccct
   104041 gccaacgccg acttaccgcg cccacgcgca gccgttaccg ctgccacgcc cggcggcatg
   104101 gtcagtagct gctgtaagag ttgctgttgt tctggttgtg gtgcgccggt cgcggggtgc
   104161 cagtcagtac ggggagtaaa atgcgccaac gagaacggct ggttttgccg ccagaggata
   104221 gcgtcgttat ccgccgtaag tacgcgtttg agatgctgga caaaatgcgg cgtcgcaata
   104281 gggtcagggc aatcactcca gcgcagcgag tcggcatcag gttggttttc ccactcttcc
   104341 catacaggga gtaacaaaac cagccagctt cccgctttca acgttccgct aagtgctgca
   104401 aaggcagcgg catcaaagcc gtggcgggcg tcgaataccg catgccggaa ctcgcgccca
   104461 agtaaagttt gtagcgccga gggtgaacag tggttttcag catctagctg cggcgaaatc
   104521 cacagccagt cgccaggtaa ggcatcacgc aacttaagag catgatcaaa acaccaaccc
   104581 tcttccccgc tcaacaccag caagcggcag atcccttcac gtttcatttg cgctgttaat
   104641 gtgtaaagcg cagtcagttc agccatccct gccccggaaa ttaaatgctt ttaccaaaag
   104701 tattgcattg tgccggatcg ccgctgtcga aaccacgttt aaaccagctg tagcgttgct
   104761 gagaagtgcc atgtgtgaaa ctgtctggta ctactcgccc ctgactttgc tgttgtaaac
   104821 ggtcatcgcc gatggcctgc gccgcattca gcgcctcttc cagatcgcca gtttccagaa
   104881 cgccttgctg ctgcatacta tgcccccaga caccggcaaa acagtcggcc tggagttcca
   104941 tacgcacaga taagcggttt acttccgcct gcgtcgcgtt ttgttgcagt tgacgaactt
   105001 tcggctcgat gcctaacagt ttctgcacat gatgaccgac ttcatgggcg ataacgtacc
   105061 cctgggcaaa atcgccatcc gcgccaagtt tgtctttcat gtcatcatag aaggagagat
   105121 cgatataaac tgtgccatcc gccgggcaat agaacggccc cataatggac tggcccgccc
   105181 cgcagccggt gcgcgtcatt ccacggtaca tgaccagctt cggttgctga taggtcttac
   105241 ccatcttttc gaactgttgt ccccaggtgt cttccgtggt tgcctgaatc accgaggtga
   105301 attttgcggc ttcgtcttca ttagggctaa ttgaccgcgt tgattgttgt tgagaaaccg
   105361 gctgcccggt catcaacccg gttaaatcaa cgccatagta gcctgcaacc agcacgacta
   105421 tcagtaaaat cagcccgcct ttaccgctcg gcagacggaa accgggaccg cccattgacg
   105481 gaccgccaga gctgttgcgc ctgtcttcaa cattgtcact ttcacgtcgc ccttgccaac
   105541 gcatagatac ctcaacaata tattcattat agaaatgatc gtaggtggtt cagaggaaga
   105601 ttaccacagg aaagcgggat gaaatggcgg aggaatgagc gttcaatgca tatattccaa
   105661 gagcggttgt cttgccgtgc cagctgcacg gcaagatgat gaacagaaaa atcagtccag
   105721 ctgtacaccc aggcggcggg cgacggcttc ataggcttcg atcaggccac cgaggctctg
   105781 gcggaaacgg tctttgtcca ttttctccag tgtttctttg tcccacaggc ggctgccgtc
   105841 cggggagaac tcatcgccca gtaccacttc acctttgtac agaccaaact ccagcttgaa
   105901 gtcgaccaga atcagaccag catcatcgaa cagttttttc agcacgtcgt tcgctttgta
   105961 ggtcagctct ttcatacgcg ccaggttctc tttgctcacc cagccaaagg tttcgcagta
   106021 agattcgttg accatcggat cgtgcatggc atcgtttttc aggaacagat cgaacagcgg
   106081 cgggttcagc tcaatacctt cttcgattcc aagacgtttc actagagagc cagcagcacg
   106141 gttacgcacg acacactcaa ccggcaccat atccagcttt ttcaccagac attcggtatc
   106201 ggagagcaga cgctccattt gagtcgggat acccgcttca gccagtttgc tcataatgaa
   106261 gtagttgaac ttgttgttca ccataccttt gcgatcaaac tgctcaatgc gcgcgccatc
   106321 ccctgctgac gtatcattgc ggaattcgag caccaacagg tccgggtttt ccgtgctgta
   106381 tacggttttc gctttaccac gatacaactc agcttgcttt tgcatcttta tcactcctgg
   106441 gtgtgaatta acgttttaaa atcttttgct gtctggtgtg ccggatgttt tgtcggatgc
   106501 ggcgtgaacg ccttatccga actacgtccg acaaacaaat ttcgtgcgaa ttaccgctaa
   106561 aatcgcgttt tcctgccgac gcacacgttt gcgtatcata tcagaaaaaa gggccggatg
   106621 attccagccc tgtattttta cttgctaaac gcagcctgga agacagctac cagcgcgtcg
   106681 ttctgactct gagtcagagt atgacctttc ggatcgatga actgtaggct gctgcggtta
   106741 tctaaatcgc caacctgcag tttatagtca ccggatgcca ggcctggatc gctcgcgccc
   106801 agttcctgcc agtcgctgtc agacagcggc ttataagtta cggccatgtt gccctgcgaa
   106861 cgggtgctgt cggtcacttt catgcccact ttttccagcg ccgctggcag acgttgccaa
   106921 accacattga acggcccgcg tacgaccagc attggtaaac cggtgtcatc agctgcactt
   106981 tgtacgtcca tagtggtgga ggcacgattt tgcgcagcgt tcgcggcgtc agtggcagat
   107041 ttatccagac cggcggaaat aacgttcatc atctccgtgc tgtaacgctg catggaagcc
   107101 gcgtctgcaa ccggtttgcc cgcctgttcc aggttcagca gtttaaccgt aaccgcctgc
   107161 tgataaccct gcggcttaac agagatttga taacgaccac gatactgctc gtcttcgtcc
   107221 agacggttcc attgtaccca atcggtggtc agtgtctgac cagcatcatc acgttgggtg
   107281 atggtgtagt ttttcgcctg cagcacgcta accacctgcg gccacagagt attgccacga
   107341 ccattttcca ccagcaatga agcggtatcg cccgtgaact gggtacgcgc gccagaaacc
   107401 agtgccaacg gctgggctgg tggacgaatg tccagcgcct taccgacagc accactaccg
   107461 ttggtcaccg ggattgcata atcaccggag gtcaccggca aaatcattcc agccggggca
   107521 tgaagctccg caagcggtgc cgcttccagg taggcttcat caccactgac ctgacgctta
   107581 tagcgtgagt cagaactaca ggcagcgagt aataaaacaa gcgaaacacc cgcaaccttt
   107641 gccaggcgcg acttttgaac agagtaagcc atcaaatctc cctaaacttt acagcaaacc
   107701 ggcatgctta agcgccgctc tgaccgtctc acgaccactg tcggtgattg gtgtcattgg
   107761 caggcgcagc gtatcggtcg ccacaagacc cagttcctta catgcccatt tcaccgggat
   107821 tggattgggt tcgacaaata gtttgttgtg taatggcatc agacgctgat taataacgcg
   107881 tgcctcggca aaatgccctt ctgctgccag tttgcacatc tgggccatat cacgcgctgc
   107941 gacgttagcc gtaacggaaa taactccatg accgcccaat tgcatgaagt ccagcgcgct
   108001 cgcatcatcg ccgctcagca gaacaaaatc atctgaaacc agctctttga tctggtttac
   108061 acgcgttaag ttccctgttg cctctttgat tccgataata ttttttactt tcgccagacg
   108121 gcccaccgtt tccgggagca gatcgcagcc agtacgggac ggcacattat acagaatttg
   108181 cggcaggtca gtatgctcag cgatggcttt gaaatgctga tacaaacctt cttgcgacgg
   108241 acgattgtag taaggggtta ccgtcaggca gccgacgata ccactgtcat tgaagcgctg
   108301 cgtcaggcta atggcttccg cagtagcgtt agcgccggtc ccggcaatta ccggaatgcg
   108361 cccatcagcc agatccagcg tcatcatcac cacatcagca tgttcgtcat gatttaaggt
   108421 agcggactcg ccagtggtgc caacagaaac gatcgccgaa gtaccgctgg cgacatgata
   108481 atcaatcagt tttttcaagc tagcccgaca gacattacct ttttcatcca tcggagtaac
   108541 aatcgcgaca atacttcccg tgaacatggg ccatcctctg tgcaaacaag tgtctcaatg
   108601 gtacgtttgg tatggcatta aaagcaagca gacagaaccg ttctgattgt tgtatgcatg
   108661 atttttttat gctttcctta agaacaactc accccttaaa ggaataacca gtttgacact
   108721 gtcatcgcaa cattatctgg tgatcactgc gttgggtgcc gatcgccctg gaattgtgaa
   108781 caccatcacc cgtcatgtca gtagttgcgg ctgtaatatt gaagacagtc gcctggcgat
   108841 gctgggagaa gagttcacgt ttatcatgct gctttccggt tcatggaatg ccatcactct
   108901 gattgaatca acgttaccgt tgaaaggtgc cgaactggat cttttaatcg tgatgaagcg
   108961 cacgacggcg cgtccgcgtc cgccaatgcc agcatctgtc tgggttcagg tcgatgttgc
   109021 agactccccg catttaattg aacgcttcac agcacttttc gacgcgcatc atatgaacat
   109081 tgcggagctg gtgtcgcgca cacaacctgc tgaaaatgaa cgggctgcgc agttgcatat
   109141 tcagataacc gcccacagcc ccgcatctgc ggacgcagca aatattgagc aagcgttcaa
   109201 agccctatgt acagaactca atgcacaagg cagtattaac gtcgtcaatt attcccaaca
   109261 tgatgaacag gatggagtta agtaatgaat ccactgaaag ccggtgatat cgcaccgaaa
   109321 tttagcttgc cggatcaaga cggagaacaa gttaatttga ccgacttcca gggacagcgt
   109381 gttctggttt atttctaccc gaaagccatg acccccggct gtaccgtaca ggcctgcggc
   109441 ttacgcgata acatggatga gttgaaaaaa gcgggcgttg atgtgctggg tatcagcacc
   109501 gataaacccg aaaaactctc ccgttttgcg gaaaaagagc tgcttaactt tacgctcctg
   109561 tctgatgagg accaccaggt gtgcgaacaa ttcggcgtct ggggtgaaaa gtccttcatg
   109621 ggcaaaacct acgatggtat tcatcgcatc agcttcctga ttgacgctga tggcaaaatc
   109681 gaacatgtct ttgacgattt caaaaccagc aatcaccacg acgttgtgct gaactggctg
   109741 aaagaacacg cctgattact ttgctccatt ccgtgctggc tgcgcttgcg gccagcatac
   109801 ctcacttctc gtgatcaaga tcacattctc gctttcccct gcgacacggg tgtcgaatcc
   109861 attttttgct gaacgttaat gaccatcatt tttgtaccgt tcagaatcca gttaatacat
   109921 aacttattga atagattgag ttaatcagaa tggcatcctt tatacaatat gaaatgcaat
   109981 gtttcatatc attttcaagg agccgacatg aaccgctttg tggtggccga accactgtgg
   110041 tgtacaggat gtaatacctg tctcgctgcc tgttcggacg tgcataaaac gcaaggttta
   110101 cagcaacacc cgcgcctggc cctggcgaag acgtcaacaa tcactgcccc tgtcgtgtgt
   110161 catcactgtg aggaagcccc ttgcctgcag gtctgcccgg tcaatgccat ctctcagagg
   110221 gatgatgcga tccaactcaa cgaaagcctc tgtattggct gcaagctttg cgccgtggtc
   110281 tgcccatttg gcgcaatcag cgcttcagga agccgtccgg tgaatgccca tgcgcaatat
   110341 gtttttcagg ctgaaggctc actcaaagac ggcgaagaaa acgtgccgcc ccaacatgcg
   110401 ttgctgcgct gggaaccggg tgtccagacc gtcgcggtga aatgcgacct gtgtgatttc
   110461 ttgccagaag gtccggcctg cgttcgcgct tgcccgaatc aggcgttacg gctgatcacc
   110521 gacgatagcc tgcaacgtca gatgaaagaa aaacagcgcc ttgccgcaag ctggtttgcc
   110581 aatggcgggg agtctcccct ttccctcact caggagcaac actaatggat gccctgcaat
   110641 tattaacctg gtcgctgatc ctctatctgt ttgccagtct ggcttcgctg tttttactcg
   110701 gtctggacag actggctatt aagctttccg gcatcacatc gctggtgggc ggcgtgattg
   110761 gcatcatcag cggaattacg caattacatg ctggtgtaac tttagtcgcc cattttgcca
   110821 ccccttttga ctttgccgat ttaaccctgc gaatggatag cctctcggca tttatggtgc
   110881 tagttatctc cttgctggtg gtggtttgtt cgctctattc attgacttat atgcgcgaat
   110941 acgagggcaa aggcgcggcg gcgatgggct tctttatgaa tcttttcatc gcatcgatgg
   111001 ttgccctgct ggtgatggac aacgcttttt ggttcatcgt gctgtttgaa atgatgtcgc
   111061 tgtcttcctg gtttctggtc attgccaggc aggataaaac gtcgatcaac gctggcatgc
   111121 tctacttttt tatcgcccac gccggatcgg tgctgattat gatcgccttc ttgctgatgg
   111181 ggcgcgaaag cggcagcctc gattttgcca gtttcgcacg ctttcacttt ctccggggct
   111241 ggcgtcggcg gtgtttctgc tggccttttt cggttttggc gcgaaagccg ggatgatgcc
   111301 gttgcacagc tggttgccgc gcgctcaccc tgccgcacca tcgcacgctt cggcgttgat
   111361 gtctggcgta atggtcaaaa tcggtatttt cggcatcctg aaagtagcga tggatctgct
   111421 ggcgcaaacg ggtttgcctc tgtggtgggg cattctggtg atggcgatcg gcgcaatctc
   111481 cgcgctcctg ggcgtgctat atgcgctggc ggaacaggat atcaaacggc tgctggcctg
   111541 gagtaccgtc gaaaacgtcg gcattatttt gctggcagtc ggtgtggcga tggacggtct
   111601 gtcactgcac gacccgctgc tcaccgtggt tggactgctc ggcgcactgt ttcatctgct
   111661 caaccatgcg ctgttcaaag ggctgctatt tctcggcgcg gacgcgatta tttcgcgttt
   111721 gcatacccac gacatggaaa aaatgggggc actggcgaaa cggatgccgt ggacagccgc
   111781 agcatgcctg attggttgcc tcgcgatatc agccattcct ccgctgaatg gttttatcag
   111841 cgaatggtac acctggcagt cgctgttctc actaagtcgt gtggaagccg tagcgctaca
   111901 acttgcgggt cctattgcta tggtaatgct ggcagtcact ggtgggctgg cagtaatgtg
   111961 cttcgtcaaa atgtacggta ttactttctg tggtgcgccg cgcagtacac acgctgaaga
   112021 ggcacaggaa gtgccaaata cgatgatcgt cgccatgcta ctgctcgcgg cactctgcgt
   112081 attaattgcg cttagtgcca gttggctggc accgaagata atgcatattg cccatgcgtt
   112141 taccaatacc cctcccgtca ctgtcgccag cggaatagca cttgtacccg gcacgtttca
   112201 tacacgggtc actccttcat tactgttgct gttactactg gcgatgcctt tgctgccagg
   112261 cctttactgg ctgtggtgtc gttcgcgccg cgcagcgttt cgtcgcacag gagatgcctg
   112321 ggcatgcggc tacggctggg aaaacgcgat ggctccgtca ggcaatggcg tgatgcagcc
   112381 gctgcgtgtg gtcttctctg cgttatttcg tctacgacaa cagctcgacc ctacgctgag
   112441 gctaaataaa ggtcttgcgc acgtcaccgc cagggctcag agcacagaac ccttctggga
   112501 tgagcgggtg atccgcccca tcgtgagcgc cacccaacgg ctggtcaaag aaatacagca
   112561 tctgcaaagc ggcgactttc gtctctattg cctgtatgtg gtcgccgcac tggttgtgct
   112621 gctaatcgct attgccgtct aaggaaatca ccatgagaca aactctttgc gacggatatc
   112681 tggtcatttt tgcgttagca caggccgtga ttctgctgat gctaacccca ctttttacgg
   112741 gtatttcccg gcagatacgc gcgcgtatgc actcccgccg cgggccgggg atctggcagg
   112801 attatcgcga tatccacaaa ctgtttaaac gccaggaagt tgcgccgaca tcttcaggtc
   112861 tgatgttccg cctgatgccg tgggtattaa tcagcagcat gctggtgctg gcgatggcct
   112921 taccactatt tattaccgtt tccccttttg cgggcggcgg cgatctgatc acccttatct
   112981 atcttcttgc cctgtttcgt tttttctttg ctctttccgg gctggatacc ggaagtccgt
   113041 ttgcgggagt cggtgccagt cgcgagttga cgctcggcat tctggtcgaa ccaatgctta
   113101 ttctctcact gctggtattg gcgctgatag caggttccac gcatatcgag atgatcagca
   113161 atacgctggc tatgggctgg aactcgccgc taaccaccgt actggcgtta ctggcctgtg
   113221 gttttgcctg cttcattgag atgggaaaaa ttccctttga tgttgctgaa gcagaacagg
   113281 aattacagga aggcccgctg accgaatatt ccggtgccgg gctggcgcta gcgaaatggg
   113341 ggctggggct gaaacaggtc gtgatggcag cactgtttgt ggccctgttt ctgccctttg
   113401 ggcgcgcgca agaactttct ctcgcctgcc tgctgacttc acttgtcgtt acgctgctca
   113461 aggttttgct gatttttgta ctggcctcaa tcgcagaaaa cacgctggca cgcgggcgtt
   113521 ttttactcat tcaccatgtg acctggcttg gcttcagcct tgctgcgctt gcatgggtct
   113581 tttggttaac cggtctgtaa ggagcactga cggaatatgg aaaatcttgc tctgacgacg
   113641 ttaatgctgc cttttatcgg cgcactggtc gtttcgtttt cgccacaacg tcgggccgcc
   113701 gaatgggggg ttttgttcgc cgcgctgacc acgctgtgca tgttgtcgct gatctccgcg
   113761 ttttatcagg ccgataaagt tgccgtcacg ttgacgttgg tcaacgtggg ggatgtggca
   113821 ttatttggcc tggtcattga tcgcgtgagt acgctgattc tgtttgtggt ggtgtttctc
   113881 ggtttgctgg tcacgatcta ctccacgggt tatctgacgg ataaaaatcg cgaacacccg
   113941 cataacggca cgaatcgtta ttacgcattt ttgctggtgt ttatcggcgc gatggcggga
   114001 ctggtactct cctcgacgct gctcggtcag ttgttgtttt ttgaaattac gggcggctgc
   114061 tcctgggcgt tgatcagtta ttaccagagc gataaagcgc agcgttcagc actaaaagcg
   114121 ttgcttatca cccatatcgg ttcgttgggg ttgtatcttg ccgccgccac gctgtttttg
   114181 cagaccggaa cgtttgtgct tagcgcgatg agcgagttac atggcgacgc acgttatctg
   114241 gtttatggcg gcatcctgtt tgccgcgtgg gggaaatcgg cccagctacc gatgcaagcg
   114301 tggctaccgg acgcaatgga agcgccaaca ccgatcagcg cctatctcca cgccgcatcg
   114361 atggtgaaag tgggcgttta catttttgcc cgcgccatta tcgacggcgg caatatcccg
   114421 catgtgattg gcggcgttgg catggtcatg acactggtca ccattcttta tggctttctg
   114481 atgtatttgc cacagcagga tatgaagcgg ttgctggcct ggtcgaccat cactcaactt
   114541 ggctggatgt tcttcggctt gtcgctctcc atcttcggct cgcggctggc gctggagggt
   114601 agcatcgcct acatcgtcaa ccacgcgttc gctaaaagcc tgtttttcct tgtagcaggt
   114661 gcgctgagtt acagctgcgg cacgcgcttg ttgccgcgtc tgcgtggcgt attgcacacc
   114721 ctgccgttgc caggcgtggg tttctgcgtg gcagcgctgg caattaccgg tgtgccgccg
   114781 ttcaacggct tcttcagtaa attcccgctg tttgccgccg gttttgcgtt gtcagtggag
   114841 tactggatcc tgctgcccgc catgattctg ctgatgattg aatcggtcgc cagtttcgcc
   114901 tggtttattc gctggtttgg tcgcgtcgtg ccgggcaaac cgagcgaggc cgtcgccgat
   114961 gccgcaccgc tgccagggtc aatgcgcctg gtgttgattg tactgattgt gatgtcgctg
   115021 atttccagcg taatcgccgc gacctggttg cagtaaggag atgatgaatg accggttcta
   115081 tgatcgtaaa taatctggcg ggactgatga tgctgacatc gctgtttgtg attagcgtca
   115141 aaagctatcg cctgtcatgc ggattttacg cctgccagtc gctggtgctg gtgtctattt
   115201 tcgccactct ctcgtgcctg ttcgccacag agcaactgct gatctggtcc gccagcgcct
   115261 ttatcaccaa agtgctgctg gtaccgttaa tcatgactta cgctgcacga aatattcccc
   115321 agaacatccc ggaaaaagcg ttattcggtc cggcaatgat ggcactgctc gcggcgttaa
   115381 ttgtcctgct ttgcgcattt gtcgttcagc ccgtgaagct accgatggct accgggctga
   115441 aaccggcgct ggcggtagcg ttaggtcatt ttctgcttgg cctgctgtgc attgtcagcc
   115501 aacgcaatat cctgcggcaa atttttggtt actgcctgat ggaaaacggc tcccatctgg
   115561 tgctggcgct tcttgcctgg cgagcaccgg aactggtgga aataggtatc gctaccgacg
   115621 ccatcttcgc cgtcattgtg atggtgttac tggcaagaaa aatatggcgt acccacggca
   115681 cgctggacgt gaacaacttg accgcgctga agggataatg agatgagtta ttctgtgatg
   115741 ttcgctttac tcctgctcac gccgctgctt ttttcgctgc tctgttttgc ctgccggaaa
   115801 cgggggctct ctgcgacttg tacggtgact gtattacata gcttagggat cacactgctg
   115861 ctgattctag cactctgggt ggtccaaact gccgctgatg caggagaaat attcgctgcg
   115921 ggactgtggc ttcatattga tggtctgggc ggtttgttcc tcgccattct tggtgtgatt
   115981 ggctttctca ccggtgttta ctcgattggc tacatgcgtc atgaagtggc acacggcgag
   116041 ctttcacccg ttacactgtg cgattactac ggtttcttcc atctgttttt gttcaccatg
   116101 ctgctggttg ttaccagcaa taacctgatt gtgatgtggg cggcgatcga agccaccacc
   116161 ttaagctcgg cgtttctggt aggcatttac ggtcagcgtt catcgctgga atcagcatgg
   116221 aagtacatca ttatttgtac tgttggtgtc gcttttggtc tgttcggtac cgtgctggta
   116281 tacgccaacg ccgccagcgt tatgccgcag gcagaaatgg cgatattctg gagcgaggtt
   116341 cttaagcaat cgtccttgct tgacccaaca ttaatgctgt tggcctttgt gtttgtgcta
   116401 attggctttg gtaccaaaac cgggctattt cccatgcacg cctggctgcc ggatgctcac
   116461 agtgaagcgc cgagtccggt cagcgccctg ctctccgccg tattgctgaa ctgcgcgctg
   116521 ttggtgctga ttcgctatta catcattatt tgccaggcca tcggcagcga tttccccaac
   116581 cggttgttgc tcatcttcgg catgttgtcg gttgccgtgg cggcattttt cattctggta
   116641 cagcgggaca ttaagcgtct gctggcgtac tccagcgtgg agaacatggg gctggtcgcg
   116701 gtggcgctag gcattggcgg gccgctggga atttttgccg cgctgctgca caccttaaac
   116761 cacagtctgg caaaaacgct gctgttctgc ggttccggca atgtactgct caagtacggc
   116821 acgcgcgatc tcaacgtcgt ctgtgggatg ctcaaaatca tgccatttac cgccgtgctg
   116881 tttggcggcg gtgcgctggc gctggcgctg gcagggatgc cgcccttcaa catttttctt
   116941 agcgaattta tgaccgttac cgcaggactg gcacgtaatc acctgctgat tatcgtcctg
   117001 ctgttattgc tgttaacgct ggtgctggcg ggcctggtac ggatggctgc gcgggtgtta
   117061 atggcgaaac cgccgcaggc cgttaaccgg ggtgatctcg gctggttgac cacctcgcca
   117121 atggtgattc tgctggtcat gatgctggcg atgggaacgc atattccaca acctgtcatc
   117181 aggatcctgg cgggcgcttc cactatagtc ctctcaggga cgcacgacct gcctgcacaa
   117241 cgtagcacct ggcatgattt tttgccttca ggcaccgcat ctgtttcgga gaaacacagt
   117301 gaacgttaat tcatcgtcaa atcgtggcga agcgattctc gccgccctga aaacgcagtt
   117361 ccccggcgcg gtgctggatg aagagcgaca aacgcctgaa caggtcacca ttacggtgaa
   117421 aatcaatctg ctgcctgacg ttgtacatta tctttattat caacatgatg gctggcttcc
   117481 agtcctgttt ggcaacgacg agcggacact taacggtcat tacgcggttt attatgccct
   117541 ttcaatggaa ggggccgaaa aatgctggat cgtggtgaag gcactggtcg atgccgacag
   117601 tcgggagttt ccgtcagtca caccgcgcgt ccctgccgcg gtctggggcg agcgagaaat
   117661 tcgcgatatg tacgggctga ttccggttgg cctgccggat cagcgtcgcc tggtgttgcc
   117721 cgatgactgg ccggaagata tgcatccgct gcgcaaagat gcgatggatt atcgactgcg
   117781 ccctgaaccg acgacagatt ccgaaacgta tgcgtttatc aatgagggca acagcgatgc
   117841 gcgggtgatc cctgtcggcc cgctgcatat cacctcctat gaaccgggtc acttccgctt
   117901 gtttgtggat ggcgagcaaa ttgtcgatgc tgattaccgc ctgttttatg tccatcgcgg
   117961 catggagaaa ctggcagaaa cgcggatggg ctacaacgaa gtgaccttct tatcggaccg
   118021 cgtgtgtggg atttgcggtt ttgcccacag tgtggcctat accaactcgg ttgaaaatgc
   118081 actggggatt gaggtgccgc aacgagcaca tactattcgc tcgattctgc tggaagtcga
   118141 gcggctacac agtcatttgc tcaaccttgg cctctcctgc cattttgttg gttttgatac
   118201 cggctttatg caatttttcc gcgtgcggga aaagtcgatg acgatggcgg aattgctgac
   118261 cgggtcgcgt aaaacctacg gtctgaatct gattggtggt gttcgccgcg atattctcaa
   118321 agagcaacgt ctgcaaacgc tgaaactggt gcgcgagatg cgcgccgacg tgtcggagct
   118381 ggtagagatg ctgcttgcta cgccgaatat ggaacaacgc actcagggca ttggcattct
   118441 cgaccgacaa atcgcccgtg attatagccc tgtagggccg ctgatccgcg cagtggtttt
   118501 gcccgtgatt tgcgctttga tcacccctac gccgactacg gcaatattcc aaaaacactg
   118561 tttaccttta ccggcggcga tgttttctcc cgcgtgatgg tccgtgtcaa agagacgttt
   118621 gattcgctgg caatgctgga atttgccctc gacaacatgc cggatacccc actgctgacc
   118681 gaaggcttta gctataaacc tcacgcattc gcgctgggct ttgttgaagc gccacgcggt
   118741 gaagacgtgc actggagcat gctcggtgat aaccaaaaat tgttccgctg gcgctgtcgt
   118801 gccgccacct acgccaactg gccggtgttg cgttacatgc tgcgcggcaa taccgtttct
   118861 gacgcaccgc tgattatcgg tagccttgat ccctgctact cctgtaccga ccgtgtgacg
   118921 ctggtagatg tgcgcaagcg ccagtcaaaa accgtgctgt ataaagagat cgaacgctac
   118981 ggcattgatc gtaaccgttc gccgctgaag taaggacaga agatgctgaa gttactgaaa
   119041 actattatgc gcgccggaac cgcgacggtg aaatatccct tcgcgccact ggaggtcagc
   119101 ccaggctttc gcgggaaacc ggacctgatg cccagccaat gtattgcctg cggtgcctgc
   119161 gcctgtgctt gtccggcaaa tgcgctgact atccagaccg acgaccagca aaattcgcgc
   119221 acctggcagc tctatctggg gcgttgtatt tactgcggac gttgtgaaga agtgtgcccg
   119281 accagagcca tccagcttac caataacttt gaactgaccg tcacccataa agccgatctc
   119341 tatacccacg cgacgttcca tctacaacgt tgcagccgtt gcgaacgccc gtttgccccg
   119401 caaaaaaccg tcgcactggc tgctgaattg ttggcacagc aacaaaatgc tccacaaaac
   119461 cgcgaaatgt tatgggcgca agcgagcgtc tgcccggagt gcaaacaacg cgcaacgctg
   119521 atcaacgacg atacagatgt accgctggtg gctaaggagc agctatgagt ccagtgctta
   119581 cgcaatatgt cagccagccc atcacgctgg acgagcaaac gcaaaagatg aagcggcatt
   119641 tgctacagga tatccgtcgc tcggcttacg tttatcgcat cgattgcggc ggctgcaacg
   119701 cctgtgaaat cgaaattttt gctgccatca cccccgtatt cgacgcagaa cgttttggca
   119761 ttaaggttgt ttcatcaccg cgtcacgccg atattttgtt atttactggc gcagtcaccc
   119821 gggcgatgcg tatgcctgca cttcgggcgt atgagtctgc ccccgatcat aaaatctgtg
   119881 tttcctacgg cgcgtgcggt gtcggcggcg gtattttcca cgatctctac agcgtctggg
   119941 gcggcagcga caccattgtc cccattgatg tttggatccc cggctgcccg ccaacaccgg
   120001 ccgccaccat tcacggtttc gccgtggcgc tcggtttgct gcaacagaag attcacgcgg
   120061 tggattatcg cgatcccacc ggggtgacta tgcaaccgtt gtggccgcag atcccgccat
   120121 cacagcgtat cgccattgag cgagaagcgc ggcggctggc gggctatcgt caggggcgag
   120181 aaatttgcga tcggctcctg cgccatttaa gcgacgatcc tacaggaaat cgggttaaca
   120241 cctggttgcg cgatgccgac gatccacgtc tcaatagtat cgttcagcaa ctctttcgcg
   120301 tactccgggg gttacatgac tgaagagtgc ggggaaattg ttttctggac gctgcgaaaa
   120361 aagtttgtcg ccagtagcga cgagatgccg gaacacagct ctcaggtaat gtattactcg
   120421 ctggctatcg gtcatcacgt tggcgtgatt gattgtctga atgtcgcctt ccgctgccca
   120481 ctgacggaat acgaagattg gcttgcactg gtcgaagagg agcaagcccg acgtaagatg
   120541 ctgggggtga tgacttttgg tgagattgtt attgacgcca gccacaccgc cctgttgacc
   120601 cgggcattcg cgccactggc agatgacgcg acgtctgtgt ggcaagcgcg tagcattcaa
   120661 ttcattcatc tgttggatga aattgtgctg gaaccggcta tctatctgat ggccagaaaa
   120721 attgcgtgag aaggatttct cattaataag gactgttgat ggctatgtca gacgaagcga
   120781 tgtttgcccc gccgcaagga ataacaattg aagcggtaaa cggaatgctc gcggagcggt
   120841 tagcgcagaa acacggcaag gcgtctttat tacgcgcctt catcccgctg ccgccgccgt
   120901 tcagcccggt acaacttatt gaactgcatg ttctcaaaag caacttctat taccgctacc
   120961 atgatgatgg cagcgatgtg acggcaacaa cagagtatca gggcgagatg gtcgattatt
   121021 cgcgttacgc cgtccttctc ggcagtagtg gaatggcgga gctacgcttt attcgtaccc
   121081 acggcagccg ttttactccc caggattgca cactgtttaa ctggctggca cgcattatca
   121141 ccccggttct gcaatcatgg ctcaatgatg aagcacagca ggtggcgctg cgtttgctgg
   121201 agaaagatcg cgatcatcat cgggtactgg tggatatcac taatgcagtg ctgtcacatc
   121261 ttgatctcga cgatctgatc gctgacgtcg ctcgtgagat ccatcatttt ttcggtctgg
   121321 cttcagtcag tatggtactg ggcgatcatc gaaagaacga gaagttcagc ctgtggtgca
   121381 gcgatctttc tgcctcacat tgtgcgtgtc tgccacacaa tatgcctggc gacagtgtat
   121441 tgctgacaca aacgctacaa acccgacaac cgaccttgac gcaccgtgca gatgatctgt
   121501 ttctctggca acgcgacccg ttattactct tacttgcatc taacggctgc gaatctgcgc
   121561 tccttatacc gcttaccttt ggcaaccata caccgggtgc attgttgctg gcgcatacct
   121621 cttccactct ctttagtgag gaaaactgcc agctactaca acacatagcc gatcgcatcg
   121681 ctattgccgt tggcaatgcc gatgcctgcc gtagaatgac cgatttgcag gaaagtttgc
   121741 agcaagaaaa ccaccagctt agcgagcagc tcctttcgaa tctgggcgtc ggtgacatta
   121801 tctatcaaag ccaggcaatg gaagacctgc tccagcaggt agatattgtg gcgaagagcg
   121861 acagtacggt gttgatttgc ggtgaaaccg gaaccggcaa agaggtgatc gccagagcga
   121921 tccatcaact tagcccgcga cacgacaagc cgctggtcaa aatcaactgc gctgccatcc
   121981 ccgccagtct tctggaaagt gagttattcg gtcatgacaa aggggcgttt actggtgcga
   122041 ttaataccca tcgtggtcgt tttgaaattg ccgatggcgg cacgttgttt ctcgatgaaa
   122101 ttggcgatct gccgttagaa cttcagccta aactgctgcg cgtattgcag gaacgggaga
   122161 ttgagcgtct cggcgggagt agaacgatcc cggtaaatgt cagagtcatt gccgccacca
   122221 accgtgattt gtggcaaatg gttgaagatc gccagtttcg cagcgatctc ttttatcgcc
   122281 tgaatgtctt cccactggaa ttgcctccgc tgcgcgaccg tccggaagat atccctcttt
   122341 tagcaaagca tttcacgcaa aaaatggcgc gccatatgaa tcgttcaatt gacgccatcc
   122401 cgaccgaggc gctacgccag ttgatgtcgt gggactggcc gggcaacgtg cgcgagctgg
   122461 aaaacgtgat tgagcgagcg gtactgctga ctcgcggtaa cagtctgaat ttacatctta
   122521 atgtccgaca aagccgttta ctgccgacgc taaatgaaga ttcagcgctt cgcagttcaa
   122581 tggcgtagtt gctgcacccg acgacgccag agaatgacga agaagaacgt cagcgcattg
   122641 ttcaggtatt gcgagaaacc aatggcattg ttgccgggcc ccgtggcgcg gcgacacgat
   122701 tagggatgaa gcgcaccacg ctgctgtcac gaatgcagcg tctgggaatc tcggttcgcg
   122761 aggtgttgta atctgctttt gcaggagtat gcatgagaaa caaactctct ttcgacttgc
   122821 agttgagcgc cagaaaagcg gcaatcgctg aacggattgc cgcccataaa attgcccgca
   122881 gtaaagtgtc ggtcttttta atggcgatgt ccgctggcgt gtttatggcg atgtccgctg
   122941 gcgtgtttat ggcgatcgga tttacttttt acctttccgt tatcgccgat gccccgtctt
   123001 cataggcatt aacccatctg gtgggcggcc tttgctttac acttggcttt attttgctgg
   123061 cggtttgcgg caccagcctg ttcacctcgt cggtaatgac ggtaatggca aaaagtcggg
   123121 gcgttattag ttggcgaact tggctgatta acgcacttct ggtggcctgc ggtaatctgg
   123181 caggtattgc ctgtttcagt ttgttaatct ggttttccgg gctggtgatg agtgaaaacg
   123241 cgatgtgggg agtcgcgatt ttacactgcg ccgagggcaa aatgcatcat acatttactg
   123301 aatctgtcag cctcggcatt atgtgcaatc tgatggtttg cctggcgctg tggatgagtt
   123361 attgcggacg ttcgttatgc gacaaaatcg tcgccatgat tttgtccatc accctgtttg
   123421 tcgccagtgg ctttgagcac tgtatcgcca atttgtttgt gattccgttc gccattgcca
   123481 ttcgccattt cgcccctacc tccttctggc aactggcgca cagtagcgca gacaattttc
   123541 cggcactgac ggtcagccat tttattaccg ccaatctgct cccggtgatg ctgggtaata
   123601 ttatcggcgg tgcagtgctg gtgagtattt gtt
//
LOCUS       DAJTRC010000002        95074 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.002, whole genome shotgun sequence.
ACCESSION   DAJTRC010000002 DAJTRC010000000
VERSION     DAJTRC010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 95074)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 95074)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..95074
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.002"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(88..1338)
                     /gene="icd"
                     /locus_tag="OO357_000120"
     CDS             complement(88..1338)
                     /gene="icd"
                     /locus_tag="OO357_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000444467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent isocitrate dehydrogenase"
                     /protein_id="HCR5885210.1"
                     /translation="MESKVVVPAQGKKITLQNGKLNVPENPIIPYIEGDGIGVDVTPA
                     MLKVVDAAVEKAYKGERKISWMEIYTGEKSTQVYGQDVWLPAETLDLIREYRVAIKGP
                     LTTPVGGGIRSLNVALRQELDLYICLRPVRYYQGTPSPVKHPELTDMVIFRENSEDIY
                     AGIEWKADSADAEKVIKFLREEMGVKKIRFPEHCGIGIKPCSEEGTKRLVRAAIEYAI
                     ANDRDSVTLVHKGNIMKFTEGAFKDWGYQLAREEFGGELIDGGPWLKVKNPNTGKEIV
                     IKDVIADAFLQQILLRPAEYDVIACMNLNGDYISDALAAQVGGIGIAPGANIGDECAL
                     FEATHGTAPKYAGQDKVNPGSIILSAEMMLRHMGWTEAADLIVKGMEGAINAKTVTYD
                     FERLMEGAKLLKCSEFGDAIIKNM"
     gene            1510..2163
                     /gene="rluE"
                     /locus_tag="OO357_000121"
     CDS             1510..2163
                     /gene="rluE"
                     /locus_tag="OO357_000121"
                     /EC_number="5.4.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415653.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2457) synthase RluE"
                     /protein_id="HCR5885211.1"
                     /translation="MRQFIISENTMQKTSFRNHQVKRFSSQRSTRRKHENQPTRVILF
                     NKPYDVLPQFTDETGRKTLKEFIPVQGVYAAGRLDRDSEGLLVLTNNGALQARLTQPG
                     KRTGKIYYVQVEGIPTQDALEALRNGVTLNDGPTLPAGAELVDEPAWLWPRNPPIRER
                     KSIPTSWLKITLYEGRNRQVRRMTAHVGFPTLRLIRYAMGDYSLDNLANGEWREVTD"
     gene            2173..2634
                     /gene="nudJ"
                     /locus_tag="OO357_000122"
     CDS             2173..2634
                     /gene="nudJ"
                     /locus_tag="OO357_000122"
                     /EC_number="3.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase NudJ"
                     /protein_id="HCR5885212.1"
                     /translation="MFKPHVTVACVVHAEGKFLVVEETINGKALWNQPAGHLEADETL
                     VEAAARELWEETGISAQPQHFIRMHQWIAPDKTPFLRFLFAIELEQICPTQPHDSDID
                     CCRWVSAEEILQASNLRSPLVAESIRCYQSGQRYPLEMIGDFNWPFTKGVI"
     gene            2688..3794
                     /gene="mnmA"
                     /locus_tag="OO357_000123"
     CDS             2688..3794
                     /gene="mnmA"
                     /locus_tag="OO357_000123"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HCR5885213.1"
                     /translation="MSETAKKVIVGMSGGVDSSVSAWLLQQQGYQVEGLFMKNWEEDD
                     GEEYCTAAADLADAQAVCDKLGIELHTVNFAAEYWDNVFELFLAEYKAGRTPNPDILC
                     NKEIKFKAFLEFAAEDLGADYIATGHYVRRADVDGKSRLLRGLDSNKDQSYFLYTLSH
                     EQIAQSLFPVGELEKPQVRKIAEDLGLVTAKKKDSTGICFIGERKFREFLGRYLPAQP
                     GKIITVDGDEIGEHQGLMYHTLGQRKGLGIGGTKEGTEEPWYVVDKDVENNILVVAQG
                     HEHPRLMSVGLIAQQLHWVDREPFTGTMRCTVKTRYRQTDIPCTVKALDDDRIKVIFD
                     EPVAAVTPGQSAVFYNGEVCLGGGIIEQRLPLPV"
     gene            3830..4471
                     /gene="hflD"
                     /locus_tag="OO357_000124"
     CDS             3830..4471
                     /gene="hflD"
                     /locus_tag="OO357_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707061.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high frequency lysogenization protein HflD"
                     /protein_id="HCR5885214.1"
                     /translation="MAKNYYDITLALAGICQSARLVQQLAHQGHCDADALHVSLNSII
                     DMNPSSTLAVFGGSEANLRVGLETLLGVLNASSCQGLNAELTRYTLSLMVLERKLSSA
                     KGALDTLGNRINGLQRQLEHFDLQSETLMSAMAAIYVDVISPLGPRIQVTGSPAVLQS
                     PQVQAKVRATLLAGIRAAVLWHQVGGGRLQLMFSRNRLTTQAKQILAHLTPEL"
     gene            4475..5845
                     /gene="purB"
                     /locus_tag="OO357_000125"
     CDS             4475..5845
                     /gene="purB"
                     /locus_tag="OO357_000125"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002950897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HCR5885215.1"
                     /translation="MELSSLTAVSPVDGRYGDKVSALRGIFSEYGLLKFRVQVEVRWL
                     QKLAAHAAIKEVPAFAADAIGYLDAIVANFSEEDAARIKTIERTTNHDVKAVEYFLKE
                     KVAEIPELHAVSEFIHFACTSEDINNLSHALMLKTARDEVILPYWRQLIDGIKDLAVQ
                     YRDIPLLSRTHGQPATPSTIGKEMANVAYRMERQHRQLNQVEILGKINGAVGNYNAHI
                     AAYPEVDWHQFSEEFVTSLGIQWNPYTTQIEPHDYIAELFDCVARFNTILIDFDRDVW
                     GYIALNHFKQKTIAGEIGSSTMPHKVNPIDFENSEGNLGLSNAVLQHLASKLPVSRWQ
                     RDLTDSTVLRNLGVGIGYALIAYQSTLKGVSKLEVNRDHLLDELDHNWEVLAEPIQTV
                     MRRYGIEKPYEKLKELTRGKRVDAEGMKQFIDGLALPEEEKARLKAMTPANYIGRAIT
                     MVDELK"
     gene            6014..6685
                     /gene="phoP"
                     /locus_tag="OO357_000126"
     CDS             6014..6685
                     /gene="phoP"
                     /locus_tag="OO357_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator PhoP"
                     /protein_id="HCR5885216.1"
                     /translation="MRVLVVEDNALLRHHLKVQIQDAGHQVDDAEDAKEADYYLNEHL
                     PDIAIVDLGLPDEDGLSLIRRWRSNDVSLPILVLTARESWQDKVEVLSAGADDYVTKP
                     FHIEEVMARMQALMRRNSGLASQVISLPPFQVDLSRRELSINDEVIKLTAFEYTIMET
                     LIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPQEVITTVRGQGY
                     LFELR"
     gene            6685..8145
                     /gene="phoQ"
                     /locus_tag="OO357_000127"
     CDS             6685..8145
                     /gene="phoQ"
                     /locus_tag="OO357_000127"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PhoQ"
                     /protein_id="HCR5885217.1"
                     /translation="MKKLLRLFFPLSLRVRFLLATAAVVLVLSLAYGMVALIGYSVSF
                     DKTTFRLLRGESNLFYTLAQWENNKLHVELPENIDKQSPTMTLIYDENGQLLWAQRDV
                     PWLMKMIQPDWLKSNGFHEIEADVNDTSLLLSGDHSIQQQLQEVREDDDDAEMTHSVA
                     VNVYPATSRMPKLTIVVVDTIPVELKSSYMVWSWFIYVLSANLLLVIPLLWVAAWWSL
                     RPIEALAKEVRELEEHNRELLNPATTRELTSLVRNLNRLLKSERERYDKYRTTLTDLT
                     HSLKTPLAVLQSTLRSLRSEKMSVSDAEPVMLEQISRISQQIGYYLHRASMRGGTLLS
                     RELHPVAPLLDNLTSALNKVYQRKGVNISLDISPEISFVGEQNDFVEVMGNVLDNACK
                     YCLEFVEISARQTDEHLYIVVEDDGPGIPLSKREVIFDRGQRVDTLRPGQGVGLAVAR
                     EITEQYEGKIVAGESMLGGARMEVIFGRQHSAPKDE"
     gene            complement(8942..9223)
                     /locus_tag="OO357_000128"
     CDS             complement(8942..9223)
                     /locus_tag="OO357_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885218.1"
                     /translation="MTEAEILRLIRRVSGISQQADEQTTQPDSVTAENYARVVAEVMR
                     RDGIQLNDADMRDIRIRVLEMLAYNRRVELYREKEKITYHWKKPERLRR"
     gene            complement(9237..10898)
                     /locus_tag="OO357_000129"
     CDS             complement(9237..10898)
                     /locus_tag="OO357_000129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5885219.1"
                     /translation="MTAWNKYAEDVKTGKIPACKRLKQAVKRYFSDLKSPLYTFDREV
                     VERFIAFSRVCPHVKGPMRGRPIELEPWQQFAFACILGFKVKATGRRKYTSAFIEVPR
                     KNAKSTTAAILANWFLIMENGQQDIYTAAVSRDQARIVFDNARQMCLLSRPLRRRVNI
                     QAHKVIHPKSNSLLKPLAAKAATIEGTNPSLSIVDEYHLHPDNGVYSALELGMGARPE
                     GLLFAITTSGSNVVSACKQHYDYCCQILDGEEVNDSIFVLIYELDDENEVDDPAMWIK
                     ANPNIDVSVDREKLASTIQKARGIPSQWVEMMTKRFNIWCQGTTPWMGNGAWAECAGT
                     FTEEDLHGQECYAGLDLSSTSDISSVCYAFPVGKTIMLISRHYLPEFQLQNPANKNRA
                     VYRQWAKAGWIRTTPGDCIDYDRIRDDIMQDAEKFNIRLVGFDTWNATHLRTQLQGAG
                     FEVEPFPQTYLRFSPAAKSFEVFVNRRVIVHRGDPVLSWSMSNVVMQSDANANIKPNK
                     KKSPNKIDPSVAALMAFGTFQAEHEDFAFDISDSHRQKLEEFSGV"
     gene            complement(10882..11208)
                     /locus_tag="OO357_000130"
     CDS             complement(10882..11208)
                     /locus_tag="OO357_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HCR5885220.1"
                     /translation="MDDIAVKQWREKSRQLAERGDLTPADWSNLELYCVNYSIYRKAV
                     ADLAARGFSIVNSQGGESRNPALSAKSDAERVMIKMASLLGFDPISRRKNPPETEEED
                     ELDRLE"
     gene            complement(11264..11446)
                     /locus_tag="OO357_000131"
     CDS             complement(11264..11446)
                     /locus_tag="OO357_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885221.1"
                     /translation="MTLMNEREQNRLIRGLIRQRDAWKTQETGHKDKASGRAERITAK
                     RLTDRDREVMECFRNR"
     gene            complement(11430..11870)
                     /locus_tag="OO357_000132"
     CDS             complement(11430..11870)
                     /locus_tag="OO357_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707053.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5885222.1"
                     /translation="MPWQPLRRCTEPGCNRRVKSGKCEEHRRAAWRAEDARRGHRRAR
                     GYSRQWDKYRAMYLSKNPLCVRCLEKGIYTPAVVVDHIIPINGGDDVLFWPEWNHQPL
                     CQACHNQKTKWLDPATKSKRAAGGFREEEERAANRNNWMYDADE"
     gene            complement(11870..12166)
                     /locus_tag="OO357_000133"
     CDS             complement(11870..12166)
                     /locus_tag="OO357_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HCR5885223.1"
                     /translation="MTEELITLEEVKLHCRIDGDDEDQLISGYIAASLEACQIHIGRR
                     FDDGLAFTPAIKIGCMMFIAHLYENRQIVADNAKTRVPMTIGALWTAYRDVGGY"
     gene            complement(12163..12501)
                     /locus_tag="OO357_000134"
     CDS             complement(12163..12501)
                     /locus_tag="OO357_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HCR5885224.1"
                     /translation="MNIGRLRDRITIQTLKQTRDITGEILETWEDGHTLWASVNMVSS
                     KEAISSGAELAIGTVRIWIRYRKDINATSRIKVSTGPLAGRVLNIIGQPLPDAARTRL
                     EILCREGAEK"
     gene            complement(12498..13709)
                     /locus_tag="OO357_000135"
     CDS             complement(12498..13709)
                     /locus_tag="OO357_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HCR5885225.1"
                     /translation="MWPFRRKKEQRSMTLDEFMALAGTSNTGAGEYVSSGTAESLPAV
                     MNAVTVISDAVATMPCYLYLVRNEKGKEAREWLDSHPVDHILNERPNAWQTPYQFKRM
                     MIRYCLLSGNAYAVIQWGRDGFPAALHPYPPQSVNVEQTGDHNWRYCITDAYTGNIRN
                     YLPWEVLHLRYSTDDGFMGRSPVTICRESLGLGLAQQRHGASVMRDGMMAAGVITSGE
                     WLDGVKGKQALAALERYKGARNAGKTPILEGGMSYQQLGMSNQDAEWLASRRFTIEDI
                     ARMFNVSPIFLQEYSNSTYSNFSEASRAFLTMTMRPWLANFEQQIKNALLVASPVPGI
                     RYQVEFDSADLLRATPGERFATYERGIKSDVMCPNEAREREGLSPRDGGDEFSQAWKQ
                     EVKISEGEKPE"
     gene            complement(13711..14283)
                     /locus_tag="OO357_000136"
     CDS             complement(13711..14283)
                     /locus_tag="OO357_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 family phage prohead protease"
                     /protein_id="HCR5885226.1"
                     /translation="MGEKIMKSMEIRSSEITTSGAGTLTGYVVRWDKLSELLWGEFYE
                     KFQRGAFTEWLAAGNDVRGLYEHDHSMLLGRTRSGTLKLEEDETGLRFELTPPDTSTG
                     RDVIELVKRGDISGMSFGFRSRKDVWDTTTDPCVRTVLVAELYEITVTSVPAYPDSGV
                     ELARRSLYEQHPEKMPRADNRRWWADLAGV"
     gene            complement(14323..15480)
                     /locus_tag="OO357_000137"
                     /pseudo
     CDS             complement(14323..15480)
                     /locus_tag="OO357_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309617.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
     gene            complement(15784..15945)
                     /locus_tag="OO357_000138"
     CDS             complement(15784..15945)
                     /locus_tag="OO357_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000126624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PerC family transcriptional regulator"
                     /protein_id="HCR5885227.1"
                     /translation="MTAQIAAYGQLVGDPLVKQSSKGTPEPPTENYADLRNAVNRTYA
                     DMGLSKLAE"
     gene            complement(15955..16260)
                     /locus_tag="OO357_000139"
     CDS             complement(15955..16260)
                     /locus_tag="OO357_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001294167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885228.1"
                     /translation="MSEPKFGEKFYKHNGRITILQISAATPGWWVETDEGSSPVASWE
                     LCVLSYPDRDVYQDILPVISTDKGMKPVDIKNMGFQCVMLTEKMMEEMKKNNAGSLH"
     gene            complement(16257..16535)
                     /locus_tag="OO357_000140"
     CDS             complement(16257..16535)
                     /locus_tag="OO357_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707047.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885229.1"
                     /translation="MHTSGKFNKSFKKRRDRTEPKYRALDMTEHALKVAIRTIDRHAG
                     EGYAKAHPELISAFMTTTAANFATLTEREIAEAEQVTTINVKTGEVES"
     gene            complement(16824..18572)
                     /locus_tag="OO357_000141"
     CDS             complement(16824..18572)
                     /locus_tag="OO357_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707046.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5885230.1"
                     /translation="MKLAPNVKKQPRGIKHKDTEVIIFAGSDAWSHAKQWQEQDGPAS
                     GDNVPPVWLGPNQLAELDALKIVPDGKKRVRLYQAGELDLVETKKIGQKLAAADIQDA
                     NFYPEGMHVQKCENWRRYLNAERENIAAGLTMPEQKNTQLAQMADSERAQLLAERFDG
                     VCVHQESEIVHVWRGGVWCPVSTMELSREMVVIYSEHRATFSKRVINNAVEALKVIAE
                     PMGEPSGDLLPFANGALDLKTGEFSPHTPENWITTHNGIEYTPPAPGENIRDNALNFH
                     KWLEHAAGKDQRKMMRICAALYMIMANRYDWQMFIEATGDGGSGKSTFTHIASLLAGK
                     QNTVSAEMTSLDDAGGRAQVVGSRLIVLADQPKYTGEGTGIKKITGGDPVEINPKYEK
                     RFTAVIRAVVLATNNNPMIFTERAGGVARRRVIFRFDNIVSEAEKDRELPEKIAAEIP
                     VIIRRLLANFTDSEKARALLLEQRDGDEALAIKQQTDPVIEFCQFLNFLEEARGLMMG
                     GGGDSVKYTTRNSLYRVYLAFMAYAGRSKPLNVNDFGKAMKPAAKVYGHEYITRKVKG
                     VTQTNAITTDECDAFL"
     gene            complement(18569..18868)
                     /locus_tag="OO357_000142"
     CDS             complement(18569..18868)
                     /locus_tag="OO357_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5885231.1"
                     /translation="MKLKYSGLTASGNTHPKFTRGDIYRDQYGGMVTIKGVAERRITY
                     RREGYEYDCVMPVYQFRRDFTLVQAAPRSKPTSREKARANIQEIKKMLNVFRGKK"
     gene            complement(18874..19107)
                     /locus_tag="OO357_000143"
     CDS             complement(18874..19107)
                     /locus_tag="OO357_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885232.1"
                     /translation="MRDIYHETIDRAFLALSHSENMLEILRIWLETLGDNERDKQKSR
                     IATALITLLEPVIMELQEIDLLHDRYKEQHTGE"
     gene            complement(19109..19329)
                     /locus_tag="OO357_000144"
                     /pseudo
     CDS             complement(19109..19329)
                     /locus_tag="OO357_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001403028.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(19322..19543)
                     /locus_tag="OO357_000145"
     CDS             complement(19322..19543)
                     /locus_tag="OO357_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001372127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885233.1"
                     /translation="MHNKTTPNAAAAALTTLMHALIDIECTAELAQGEEQKDRTQFAL
                     ECIRYIATRSLNDAKNILVADCENGGGYA"
     gene            complement(19533..19733)
                     /locus_tag="OO357_000146"
     CDS             complement(19533..19733)
                     /locus_tag="OO357_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000226783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host cell division inhibitor Icd-like protein"
                     /protein_id="HCR5885234.1"
                     /translation="MVAVNHIPHLVHTQTAFVWRFLALSAGESQIIHVTAWTEREARS
                     RCPSGCVAVFAARIRQGETYAQ"
     gene            19863..20042
                     /locus_tag="OO357_000147"
     CDS             19863..20042
                     /locus_tag="OO357_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000103622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885235.1"
                     /translation="MSTHKNERRGNPPFQFRLDPDLREKMEKAQQQDGDDSLAAWIKR
                     IIRKELQQRGIEPKG"
     gene            20167..20496
                     /locus_tag="OO357_000148"
     CDS             20167..20496
                     /locus_tag="OO357_000148"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885236.1"
                     /translation="MNQNDSRRVLLPLRSTLKNTHQRRELRSYGNALNQTDCIAHQPD
                     SGYHPRAYVWDHTHKRRSGLSVQKGGSISGPLFLCLKNPNFVVFQFHQGERIPAHVLA
                     YIQSSWL"
     gene            complement(20546..20734)
                     /locus_tag="OO357_000149"
     CDS             complement(20546..20734)
                     /locus_tag="OO357_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5885237.1"
                     /translation="MLSTDRFIREKECEKLTGLSRSCRYRLEKAGQFPSRRKLGGRSV
                     GWSLSEVLAWKDSCKAVH"
     gene            complement(21109..22338)
                     /locus_tag="OO357_000150"
     CDS             complement(21109..22338)
                     /locus_tag="OO357_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5885238.1"
                     /translation="MSRALNKLSDTQLRKINGTPAQKTAFLNDGGNLSVRHSTSGLLT
                     WYFTYRAGTGRGAPPERIKLGNYPDLSLKSAREKAAQCRAWLAEGKNPRHELNYTVQE
                     ALKPVTVGDALTYWLESYAKENRVDYAALKKRLNNHVIQHIGAMPLDKCELRHWLACF
                     DQVAKRTPVTAGFLLQTCKQALKFCRRRRYAISNVLDDMSVADVGKKPDISERVLSTK
                     ELGELLQALDKKIFSPYYIALIRLLIVFGCRTVELRLSEISEWDFTEMLWTVPKEHSK
                     TKVAIFRPIPEAILPFVTQLVEQNRHTGLLLGEVKQETSVSQYGRLAHRRLNHPHWSL
                     HDIRRTFTTMLNDLGVDPHVVEQLTGHQMPGMQRVYNHSRYLDAKRNALDMWTERLGI
                     LAGTHENVTTLPVARRK"
     gene            22587..23708
                     /gene="roxA"
                     /locus_tag="OO357_000151"
                     /gene_synonym="ycfD"
     CDS             22587..23708
                     /gene="roxA"
                     /locus_tag="OO357_000151"
                     /gene_synonym="ycfD"
                     /EC_number="1.14.11.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309600.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[50S ribosomal protein L16]-arginine
                     3-hydroxylase"
                     /protein_id="HCR5885239.1"
                     /translation="MEYQLTLNWPDFLERHWQKRPVVLKRGFNNFIDPISPDELAGLA
                     MESEVDSRLVSHQDGKWQVSHGPFESYDHLGETNWSLLVQAVNHWHEPTAALMRPFRE
                     LPDWRIDDLMISFSVPGGGVGPHLDQYDVFIIQGTGRRRWRVGEKLQMKQHCPHPDLL
                     QVDPFEAIIDEELEPGDILYIPPGFPHEGYALENAMNYSVGFRAPNTRELISGFADYV
                     LQRELGGNYYSDPDVPPRAHPADVLPQEMDKLREMMLELINQPEHFKQWFGEFISQSR
                     HELDIAPPEPPYQPDEIYDALKQGDVLVRLGGLRVLRIGDDVYANGEKIDSPHRPALD
                     ALASNIALTAENFGDALEDPSFLAMLAALVNSGYWFFEG"
     gene            complement(23757..24983)
                     /gene="pepT"
                     /locus_tag="OO357_000152"
     CDS             complement(23757..24983)
                     /gene="pepT"
                     /locus_tag="OO357_000152"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HCR5885240.1"
                     /translation="MDKLLERFLNYVSLDTQSKAGVRQVPSTEGQWKLLHLLKEQLEE
                     MGLINVTLSEKGTLMATLPANVPGDIPAIGFISHVDTSPDCSGKNVNPQIVENYRGGD
                     IALGIGDEVLSPVMFPVLHQLLGQTLITTDGKTLLGADDKAGIAEIMTALAVLQQKNI
                     PHGDIRVAFTPDEEVGKGAKHFDVDAFDARWAYTVDGGGVGELEFENFNAASVNIKIV
                     GNNVHPGTAKGVMVNALSLAARIHAEVPADESPEMTEGYEGFYHLASMKGTVERADMH
                     YIIRDFDRKQFEARKRKMMEIAKKVGKGLHPDCYIELVIEDSYYNMREKVVEHPHILD
                     IAQQAMRDCDIEPELKPIRGGTDGAQLSFMGLPCPNLFTGGYNYHGKHEFVILEGMEK
                     AVQVIVRIAELTAQRK"
     gene            25233..26369
                     /gene="potA"
                     /locus_tag="OO357_000153"
     CDS             25233..26369
                     /gene="potA"
                     /locus_tag="OO357_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     ATP-binding protein PotA"
                     /protein_id="HCR5885241.1"
                     /translation="MGQSKKLNKQPSSLSPLVQLAGIRKCFDGKEVIPQLDLTINNGE
                     FLTLLGPSGCGKTTVLRLIAGLETVDSGRIMLDNEDITHVPAENRYVNTVFQSYALFP
                     HMTVFENVAFGLRMQKTPAAEITPRVMEALRMVQLETFAQRKPHQLSGGQQQRVAIAR
                     AVVNKPRLLLLDESLSALDYKLRKQMQNELKALQRKLGITFVFVTHDQEEALTMSDRI
                     VVMRDGRIEQDGTPREIYEEPKNLFVAGFIGEINMFNATVIERLDEQRVRANVEGREC
                     NIYVNFAVEPGQKLHVLLRPEDLRVEEINDDNHAEGLIGYVRERNYKGMTLESVVELE
                     NGKMVMVSEFFNEDDPDFDHSLDQKMAINWVESWEVVLADEEHK"
     gene            26353..27210
                     /gene="potB"
                     /locus_tag="OO357_000154"
     CDS             26353..27210
                     /gene="potB"
                     /locus_tag="OO357_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000799378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotB"
                     /protein_id="HCR5885242.1"
                     /translation="MKNTSKFQNVVIVTIVGWLVLFVFLPNLMIIGTSFLTRDDASFV
                     KMVFTLDNYTRLLDPLYFEVLLHSLNMALIATLACLVLGYPFAWFLAKLPHKVRPLLL
                     FLLIVPFWTNSLIRIYGLKIFLSTKGYLNEFLLWLGVIDTPIRIMFTPSAVIIGLVYI
                     LLPFMVMPLYSSIEKLDKPLLEAARDLGASKLQTFIRIIIPLTMPGIIAGCLLVMLPA
                     MGLFYVSDLMGGAKNLLIGNVIKVQFLNIRDWPFGAATSITLTIVMGLMLLVYWRASR
                     LLNKKVELE"
     gene            27207..28001
                     /gene="potC"
                     /locus_tag="OO357_000155"
     CDS             27207..28001
                     /gene="potC"
                     /locus_tag="OO357_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter permease
                     PotC"
                     /protein_id="HCR5885243.1"
                     /translation="MIGRLLRGGFMTAIYAYLYIPIIILIVNSFNSSRFGINWQGFTT
                     KWYSLLMNNDSLLQAAQHSLTMAVFSATFATLIGSLTAVALYRYRFRGKPFVSGMLFV
                     VMMSPDIVMAISLLVLFMLLGIQLGFWSLLFSHITFCLPFVVVTVYSRLKGFDVRMLE
                     AAKDLGASEFTILRKIILPLAMPAVAAGWVLSFTLSMDDVVVSSFVTGPSYEILPLKI
                     YSMVKVGVSPEVNALATILLVLSLVMVIASQLIARDKTKGNTGDVK"
     gene            27998..29044
                     /gene="potD"
                     /locus_tag="OO357_000156"
     CDS             27998..29044
                     /gene="potD"
                     /locus_tag="OO357_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotD"
                     /protein_id="HCR5885244.1"
                     /translation="MKKWSRHLLAAGALALGMSAAHADDNNTLYFYNWTEYVPPGLLE
                     QFTKETGIKVIYSTYESNETMYAKLKTYKDGAYDLVVPSTYYVDKMRKEGMIQKIDKS
                     KLTNFSNLDPDMLNKPFDPNNDYSIPYIWGATAIGVNGDAVDPKSVTSWADLWKPEYK
                     GSLLLTDDAREVFQMALRKLGYSGNTTDPKEIEAAYNELKKLMPNVAAFNSDNPANPY
                     MEGEVNLGMIWNGSAFVARQAGTPIDVVWPKEGGIFWMDSLAIPANAKNKEGALKLIN
                     FLLRPDVAKQVAETIGYPTPNLAARKLLSPEVANDKTLYPDAETIKNGEWQNDVGAAS
                     SIYEEYYQKLKAGR"
     gene            complement(29200..30021)
                     /gene="cobB"
                     /locus_tag="OO357_000157"
     CDS             complement(29200..30021)
                     /gene="cobB"
                     /locus_tag="OO357_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HCR5885245.1"
                     /translation="MLSRRGHRLSRFRKNKRRLRERLRQRIFFRDKVVPEAMEKPRVL
                     VLTGAGISAESGIRTFRAADGLWEEHRVEDVATPEGFDRDPELVQAFYNARRRQLQQP
                     EIQPNAAHLALAKLQDALGDRFLLVTQNIDNLHERAGNTNVIHMHGELLKVRCSQSGQ
                     VLDWTGDVTPEDKCHCCQFPAPLRPHVVWFGEMPLGMDEIYMALSMADVFIAIGTSGH
                     VYPAAGFVHEAKLHGAHTVELNLEPSQVGNEFAEKYYGPASQVVPEFVEKLLEGL"
     gene            complement(30037..30948)
                     /gene="nagK"
                     /locus_tag="OO357_000158"
     CDS             complement(30037..30948)
                     /gene="nagK"
                     /locus_tag="OO357_000158"
                     /EC_number="2.7.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine kinase"
                     /protein_id="HCR5885246.1"
                     /translation="MYYGFDIGGTKIALGVFDSGRQLQWEKRVPTPRDSYDAFLDAVC
                     ELVAEADQRFGCKGSVGIGIPGMPETEDGKLYAANVPAASGKPLRADLSARLDRDVRL
                     DNDANCFALSEAWDDEFTQYPLVMGLILGTGVGGGLVFNGKPITGKSYITGEFGHMRL
                     PVDALTMMGLDFPLRRCGCGQHGCIENYLSGRGFAWLYQHYYHQPLQAPEIIALYDQG
                     DEQARAHVERYLDLLAVSLGNILTIVDPDLVVIGGGLSNFPAITTQLADRLPCHLLPV
                     ARVPRIERARHGDAGGMRGAAFLHLTD"
     gene            complement(30977..32221)
                     /gene="lolE"
                     /locus_tag="OO357_000159"
     CDS             complement(30977..32221)
                     /gene="lolE"
                     /locus_tag="OO357_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolE"
                     /protein_id="HCR5885247.1"
                     /translation="MAMPLSLLIGLRFSRGRRRGGMVSLISVISTIGIALGVAVLIVG
                     LSAMNGFERELNNRILAVVPHGEIEAVNQPWTNWQEALDNVQKVPGIAAAAPYINFTG
                     LVESGANLRAIQVKGVNPQQEQRLSALPSFVQGDAWRNFKAGEQQIIIGKGVADALKV
                     KQGDWVSIMIPNSNPEHKLMQPKRVRLHIAGILQLSGQLDHSFAMIPLADAQQYLDMG
                     SSVSGIALKMTDVFNANKLVRDAGEVTNSYVYIKSWIGTYGYMYRDIQMIRAIMYLAM
                     VLVIGVACFNIVSTLVMAVKDKSGDIAVLRTLGAKDGLIRAIFVWYGLLAGLFGSLCG
                     VVIGVVVSLQLTPIIEWIEKLIGHQFLSSDIYFIDFLPSELHWLDVFYVLVTALLLSL
                     LASWYPARRASNIDPARVLSGQ"
     gene            complement(32221..32922)
                     /gene="lolD"
                     /locus_tag="OO357_000160"
     CDS             complement(32221..32922)
                     /gene="lolD"
                     /locus_tag="OO357_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter
                     ATP-binding protein LolD"
                     /protein_id="HCR5885248.1"
                     /translation="MNKILLQCDNLCKRYQEGSVQTDVLLNVSFSVGEGEMMAIVGSS
                     GSGKSTLLHLLGGLDTPTSGDVIFNGQPMSKLSSAAKAELRNQKLGFIYQFHHLLPDF
                     TALENVAMPLLIGKKKPAEINSCALEMLKAVGLEHRANHRPSELSGGERQRVAIARAL
                     VNNPRLVLADEPTGNLDARNADSIFQLLGELNRLQGTAFLVVTHDLQLAKRMSRQLEM
                     RDGRLTAELSLMGAE"
     gene            complement(32915..34114)
                     /gene="lolC"
                     /locus_tag="OO357_000161"
     CDS             complement(32915..34114)
                     /gene="lolC"
                     /locus_tag="OO357_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004976588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit LolC"
                     /protein_id="HCR5885249.1"
                     /translation="MYQPVALFIGLRYMRGRAVDRFGRFVSWLSTIGITLGVMALVTV
                     LSVMNGFERELQNNILGLMPQAILSSEHGSLNPQQLPETAVKLDGVNRVAPITTGDVV
                     LQSARSVAVGVMLGIDPAQKDPLTPYLVNVKQTDLEPGKYNVILGEQLASQLGVNRGD
                     QIRVMVPSASQFTPMGRIPSQRLFNVIGTFAANSEVDGYEMLVNIEDASRLMRYPAAN
                     ITGWRLWLDEPLRVDSLSQQKLPEGSKWQDWRDRKGELFQAVRMEKNMMGLLLSLIVA
                     VAAFNIITSLGLMVMEKQGEVAILQTQGLTPRQIMMVFMVQGASAGIIGAILGAALGA
                     LLASQLNNLMPIIGVLLDGAALPVAIEPLQVIVIALVAMAIALLSTLYPSWRAAATQP
                     AEALRYE"
     gene            34376..35449
                     /locus_tag="OO357_000162"
     CDS             34376..35449
                     /locus_tag="OO357_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5885250.1"
                     /translation="MKQKELWINQIKGLCICLVVIYHSVITFYPHMTTFQHPLSEVLS
                     KCWIYFNLYLAPFRMPVFFFISGYLIRRYIDSVPWGNCLDKRIWNIFWVLALWGVVQW
                     LALSALNQWLAPERDLSNASNAAYADSTGEFLHGMITASTSLWYLYALIVYFVVCKIF
                     SRLALPLFALFVLLSVAVNFVPTPWWGMNSVIRNLPYYSLGAWFGATIMTCVKEVPLR
                     RHLLMASLLTVLAVGAWLFTISLLLSLVSIVVIMKLFYQYEQRFGMRSTSLLNVIGSN
                     TIAIYTTHRILVEIFSLTLLAQMNAARWSPQVELTLLLVYPFVSLFICTVAGLLVRKL
                     SQRAFSDLLFSPPSLPAAVSYSR"
     gene            35577..39023
                     /gene="mfd"
                     /locus_tag="OO357_000163"
     CDS             35577..39023
                     /gene="mfd"
                     /locus_tag="OO357_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HCR5885251.1"
                     /translation="MPEQYRYTLPVKAGEQRLLGELTGAACATLVAEIAERHAGPVVL
                     IAPDMQNALRLHDEISQFTDQMVMNLADWETLPYDSFSPHQDIISSRLSTLYQLPTMQ
                     RGILIVPVNTLMQRVCPHSFLHGHALVMKKGQRLSRDALRTQLDSAGYRHVDQVMEHG
                     EYATRGALLDLFPMGSELPYRLDFFDDEIDSLRVFDVDSQRTLEEVEAINLLPAHEFP
                     TDKAAIELFRSQWRDTFEVKRDPEHIYQQVSKGTLPAGIEYWQPLFFSEPLPPLFSYF
                     PANTLLVNTGDLETSAERFQADTLARFENRGVDPMRPLLPPQSLWLRVDELFSELKNW
                     PRVQLKTEHLPTKAANANLGFQKLPDLAVQAQQKAPLDALRKFLETFDGPVVFSVESE
                     GRREALGELLARIKIAPQRIMRLDEASDRGRYLMIGAAEHGFVDKARNLALICESDLL
                     GERVARRRQDSRRTINPDTLIRNLAELHIGQPVVHLEHGVGRYAGMTTLEAGGITGEY
                     LMLTYANDAKLYVPVSSLHLISRYAGGAEENAPLHKLGGDAWSRARQKAAEKVRDVAA
                     ELLDIYAQRAAKEGFAFKHDREQYQLFCDSFPFETTPDQAQAINAVLSDMCQPLAMDR
                     LVCGDVGFGKTEVAMRAAFLAVDNHKQVAVLVPTTLLAQQHYDNFRDRFANWPVRIEM
                     ISRFRSAKEQTQILAEVAEGKIDILIGTHKLLQSDVKFKDLGLLIVDEEHRFGVRHKE
                     RIKAMRANVDILTLTATPIPRTLNMAMSGMRDLSIIATPPARRLAVKTFVREYDSLVV
                     REAILREILRGGQVYYLYNDVENIQKAAERLAELVPEARIAIGHGQMRERELERVMND
                     FHHQRFNVLVCTTIIETGIDIPTANTIIIERADHFGLAQLHQLRGRVGRSHHQAYAWL
                     LTPHPKAMTTDAQKRLEAIASLEDLGAGFALATHDLEIRGAGELLGEEQSGSMETIGF
                     SLYMELLENAVDALKAGREPSLEDLTSQQTEVELRMPSLLPDDFIPDVNTRLSFYKRI
                     ASAKTENELEEIKVELIDRFGLLPDPARTLLDIARLRQQAQKLGIRKLEGNEKGGVIE
                     FAEKNHVNPAWLIGLLQKQPQHYRLDGPTRLKFIQDLSERKTRIEWVRQFMRELEENA
                     IA"
     gene            39166..40128
                     /gene="ldtC"
                     /locus_tag="OO357_000164"
     CDS             39166..40128
                     /gene="ldtC"
                     /locus_tag="OO357_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707028.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtC"
                     /protein_id="HCR5885252.1"
                     /translation="MMIKTRFSRWLTFFTFAAAVALALPAKANTWPLPPASSRLVGEN
                     KFHVVENDGGSLEAIAKKYNVGFLALLQANPGVDPYVPRAGSVLTIPLQTLLPDAPRE
                     GIVINIAELRLYYYPPGKNSVTVYPIGIGQLGGDTLTPTMVTTVSDKRANPTWTPTAN
                     IRARYKAQGIELPAVVPAGPDNPMGHHAIRLAAYGGVYLLHGTNADFGIGMRVSSGCI
                     RLRDDDIKTLFSQVTPGTKVNIINTPIKVSAEPNGARLVEVHQPLSEKIDDDPQLLPI
                     TLNSAMQSFKDAAQTDAEVMQHVMDVRSGMPVDVRRHQVSPQTL"
     gene            complement(40211..40423)
                     /locus_tag="OO357_000165"
     CDS             complement(40211..40423)
                     /locus_tag="OO357_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015705050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5885253.1"
                     /translation="MSFANFAAVEVQSTPEGQQKVGTISANAGTNLGSLEEQLAQKAD
                     EMGAKSFRITSVTGPNTLHGTAVIYK"
     gene            40708..41340
                     /gene="comR"
                     /locus_tag="OO357_000166"
     CDS             40708..41340
                     /gene="comR"
                     /locus_tag="OO357_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family copper-responsive transcriptional
                     repressor ComR"
                     /protein_id="HCR5885254.1"
                     /translation="MATDSTQCVKKSRGRPKVFDRDAALDKAMKLFWQHGYEATSLAD
                     LVEATGAKAPTLYAEFTNKEGLFRAVLDRYIDRFAAKHEAQLFCEEKSVESALADYFA
                     AIANCFTSKDTPAGCFMINNCTTLSPDSGDIANTLKSRHAMQERTLQQFLCQRQARGE
                     IPTHCDVTHLAEFLNCIIQGMSISAREGASLEKLMQIAGTTLRLWPELVK"
     gene            complement(41402..41941)
                     /locus_tag="OO357_000167"
     CDS             complement(41402..41941)
                     /locus_tag="OO357_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5885255.1"
                     /translation="MNKSMLAGIGIGVAAALGVAAVASLNVFERGPQYAQVVSATPIK
                     ETVKTPRQECRNVTVTHRRPVQDENRITGSVLGAVAGGVIGHQFGGGRGKDVATVVGA
                     LGGGYAGNQIQGSLQESDTYTTTQQRCKTVYDKSEKMLGYDVTYKIGDQQGKIRMDRD
                     PGTQIPLDSNGQLILNNKV"
     gene            complement(42168..43472)
                     /gene="ndh"
                     /locus_tag="OO357_000168"
     CDS             complement(42168..43472)
                     /gene="ndh"
                     /locus_tag="OO357_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017384716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone dehydrogenase"
                     /protein_id="HCR5885256.1"
                     /translation="MTTPLKKIVIVGGGAGGLEMATQLGHKLGRKKKAKITLVDRNHS
                     HLWKPLLHEVATGSLDEGVDALSYLAHARNHGFQFQLGSVIDIDREAKTITIAELRDE
                     KGELLVPERKIAYDTLVMALGSTSNDFNTPGVKENCIFLDNPHQARRFHQEMLNLFLK
                     YSANLGANGKVNIAIVGGGATGVELSAELHNAIKQLHSYGYKGLTNEALNVTLVEAGE
                     RILPALPPRISAAAHNELTKLGVRVLTQTMVTSADEGGLHTKDGEYIEADLMVWAAGI
                     KAPDFLKDIGGLETNRINQLVVEPTLQTTRDPDIYAIGDCASCPRPEGGFVPPRAQAA
                     HQMATCAMNNILAQMNGKPLKNYQYKDHGSLVSLSNFSTVGSLMGNLTRGSMMIEGRI
                     ARFVYISLYRMHQIALHGYFKTGLMMLVGSINRVIRPRLKLH"
     gene            complement(43880..44422)
                     /locus_tag="OO357_000169"
     CDS             complement(43880..44422)
                     /locus_tag="OO357_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5885257.1"
                     /translation="MIIYLHGFDSNSPGNHEKVLQLQFIDPDVRLISYSTRHPKHDMQ
                     HLLKEVDKMLQLNVDERPLICGVGLGGYWAERIGFLCDIRQVIFNPNLFPYENMEGKI
                     DRPEEYADIATKCVTNFREKNRDRCLVILSRNDEALNSQRTSEELHHYYEIVWDEEQT
                     HKFKNISPHLQRIKAFKTLG"
     gene            complement(44445..45470)
                     /gene="nagZ"
                     /locus_tag="OO357_000170"
     CDS             complement(44445..45470)
                     /gene="nagZ"
                     /locus_tag="OO357_000170"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HCR5885258.1"
                     /translation="MGPVMLDVEGYELDAEEREILAHPLVGGLILFTRNYHDPAQLRE
                     LVRQIRAASRNHLVVAVDQEGGRVQRFREGFTRLPAAQSFAALLGMEEGGKLAQEAGW
                     LMASEMIAMDIDISFAPVLDVGHISAAIGERSYHADPQKALAIASRFIDGMHEAGMKT
                     TGKHFPGHGAVTADSHKETPCDPRPQAEIRAKDMSVFSSLIRENKLDAIMPAHVIYSD
                     VDPRPASGSPYWLKTVLRQELGFDGVIFSDDLSMEGAAIMGSYAERGQASLDAGCDMI
                     LVCNNRKGAVSVLDNLSPIKAERVTRLYHKGSFSRQELMDSARWKAISTRLNQLHERW
                     QEEKVGH"
     gene            complement(45481..46305)
                     /gene="thiK"
                     /locus_tag="OO357_000171"
     CDS             complement(45481..46305)
                     /gene="thiK"
                     /locus_tag="OO357_000171"
                     /EC_number="2.7.1.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine kinase"
                     /protein_id="HCR5885259.1"
                     /translation="MPFRSNNPITRDELLSRFFPQFHPVTTFNSGLSGGSFLIEHQGQ
                     RFVVRQPHDPDAPQSAFLRQYRALSQLPASIAPKPHLYLRDWMVVDYLPGAVKTYLPD
                     TNELAGLLYYLHQQPRFGWRITLLPLLELYWQQSDPARRTVGWLRMLKRLRKAREPRP
                     LRLSPLHMDVHAGNLVHSASGLKLIDWEYAGDGDIALELAAVWVENTEQHRQLVNDYA
                     TRAKIYPAQLWRQVRRWFPWLLMLKGGWFEYRWRQTGDQQFIRLADDTWRQLLIKQ"
     gene            complement(46286..46927)
                     /gene="lpoB"
                     /locus_tag="OO357_000172"
     CDS             complement(46286..46927)
                     /gene="lpoB"
                     /locus_tag="OO357_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoB"
                     /protein_id="HCR5885260.1"
                     /translation="MTKMSRYALITALAMFLAGCVGQREPAPVEEVKPAPEQPAEPQQ
                     PVPTVPSVPTIPQQPGPIEHEDRTAPPAPHIRHYDWNGAMQPMVSKMLGADGVTAGSV
                     LLVDSVNNRTNGSLNAAEATETLRNALANNGKFTLVSAQQLSMAKQQLGLSPQDSLGT
                     RSKAIGIARNVGAHYVLYSCASGNVNAPTLQMQLMLVQTGEIIWSGKGAVSQQ"
     gene            complement(46942..47319)
                     /locus_tag="OO357_000173"
     CDS             complement(46942..47319)
                     /locus_tag="OO357_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcfL family protein"
                     /protein_id="HCR5885261.1"
                     /translation="MRKGCFGLVSLVLLLLVGCRSHPEIPVNDEQSLVMESSLLAAGI
                     SAEKPVLSTSDIQPSASSTIYNERQEPVTVHYRFYWYDARGLEMHPLERPRSVTIPAH
                     SAVTLYGSANFLGAHKVRLYLYL"
     gene            complement(47322..47681)
                     /gene="hinT"
                     /locus_tag="OO357_000174"
     CDS             complement(47322..47681)
                     /gene="hinT"
                     /locus_tag="OO357_000174"
                     /EC_number="3.9.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415621.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleoside phosphoramidase"
                     /protein_id="HCR5885262.1"
                     /translation="MAEETIFSKIIRREIPSDIVYQDDLVTAFRDISPQAPTHILIIP
                     NILIPTVNDVSAEHEQALGRMITVAAKIAEQEGIAEDGYRLIMNTNRHGGQEVYHIHM
                     HLLGGRPLGPMLAHKGL"
     gene            48014..50199
                     /gene="fhuE"
                     /locus_tag="OO357_000175"
                     /pseudo
     CDS             48014..50199
                     /gene="fhuE"
                     /locus_tag="OO357_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ferric-rhodotorulic acid/ferric-coprogen
                     receptor FhuE"
     gene            complement(50259..51692)
                     /gene="ptsG"
                     /locus_tag="OO357_000176"
     CDS             complement(50259..51692)
                     /gene="ptsG"
                     /locus_tag="OO357_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000475716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIBC"
                     /protein_id="HCR5885263.1"
                     /translation="MFKNAFANLQKVGKSLMLPVSVLPIAGILLGVGSANFSWLPAVV
                     SHVMAEAGGSVFANMPLIFAIGVALGFTNNDGVSALAAVVAYGIMVKTMAVVAPLVLH
                     LPAEEIASKHLADTGVLGGIISGAIAAYMFNRFYRIKLPEYLGFFAGKRFVPIISGLA
                     AIFTGVVLSFIWPPIGSAIQTFSQWAAYQNPVVAFGIYGFIERCLVPFGLHHIWNVPF
                     QMQIGEYTNAAGQVFHGDIPRYMAGDPTAGKLSGGFLFKMYGLPAAAIAIWHSAKPEN
                     RAKVGGIMISAALTSFLTGITEPIEFFFMFVAPILYIIHAILAGLAFPICILLGMRDG
                     TSFSHGLIDFIVLSGNSSKLWLLPIVGIGYAIVYYTIFRVLIKALDLKTPGREDATED
                     AKATGTSEMAPALVAAFGGKENITNLDACITRLRVSVADVSKVDQAGLKKLGAAGVVV
                     AGSGVQAIFGTKSDNLKTEMDEYIRNH"
     gene            complement(51987..52784)
                     /locus_tag="OO357_000177"
     CDS             complement(51987..52784)
                     /locus_tag="OO357_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5885264.1"
                     /translation="MFLVDSHCHLDGLDYESLHKDVDDVLAKAAARDVKFCLAVATTL
                     PGYLHMRDLVGERDNVVFSCGVHPLNQNDPYDVEDLRRLAAEEGVVALGETGLDYYYT
                     PETKVRQQESFIHHIQIGRELNKPVIVHTRDARADTLAILREEKVTDCGGVLHCFTED
                     RETAGKLLDLGFYISFSGIVTFRNAEQLRDAARYVPLDRLLVETDSPYLAPVPHRGKE
                     NQPAMVRDVAEYMAVLKGVAVEELAQVTTDNFARLFHIDASRLQSIR"
     gene            complement(52795..53799)
                     /gene="holB"
                     /locus_tag="OO357_000178"
     CDS             complement(52795..53799)
                     /gene="holB"
                     /locus_tag="OO357_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HCR5885265.1"
                     /translation="MRWYPWLRPDFEKLVASYQAGRGHHALLIQALPGMGDDALIYAL
                     SRYLLCQQPQGHKSCGHCRGCQLMQAGTHPDYYTLAPEKGKNTLGIDAVREVTEKLNE
                     HARLGGAKVVWVTDAALLTDAAANALLKTLEEPPAETWFFLATREPERLLATLRSRCR
                     LHYLAPPPEQYAVTWLSREVTMSQDALLAALRLSAGSPGAALALFQGDNWQARETLCQ
                     ALAYSVPSGDWYSLLAALNHEQAPARLHWLATLLMDALKRHHGAAQVTNVDVPGLVAE
                     LANHLSPSCLQAILGDVCHIREQLMSVTGINRELLITDLLLRIEHYLQPGVVLPVPHL
                     "
     gene            complement(53796..54437)
                     /locus_tag="OO357_000179"
     CDS             complement(53796..54437)
                     /locus_tag="OO357_000179"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HCR5885266.1"
                     /translation="MRSKYIVIEGLEGAGKTTARNVVVETLEQLGIRDMVFTREPGGT
                     QLAEKLRSLVLDIKSVGDEVITDKAEVLMFYAARVQLVETVIKPALANGTWVIGDRHD
                     LSTQAYQGGGRGIDQHMLATLRDAVLGDFRPDLTLYLDVTPEVGLKRARARGELDRIE
                     QESFDFFNRTRARYLELAAQDKSIHTIDATQPLEAVMDAIRTTVTHWVKELDA"
     gene            complement(54427..55449)
                     /gene="yceG"
                     /locus_tag="OO357_000180"
     CDS             complement(54427..55449)
                     /gene="yceG"
                     /locus_tag="OO357_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein YceG"
                     /protein_id="HCR5885267.1"
                     /translation="MKKVLLIILLLLVVLGIAAGVGVWKVRHLADSKLLIKEETIFTL
                     KPGTGRLALGEQLYADKIINRPRVFQWLLRIEPDLSHFKAGTYRFTPQMTVREMLKLL
                     ESGKEAQFPLRLVEGMRLSDYLKQLREAPYIKHTLSDDKYATVAQALELENPEWIEGW
                     FWPDTLMYTANTTDVALLKRVHKKMVKAVDSAWEGRADGLPYKDKNQLVTMASIIEKE
                     TAVASERDQVASVFINRLRIGMRLQTDPTVIYGMGERYNGKLSRADLETPTAYNTYTI
                     TGLPPGAIATPGADSLKAAAHPAKTPYLYFVADGKGGHTFNTNLASHNKSVQDYLKVL
                     KEKNAQ"
     gene            complement(55452..56261)
                     /gene="pabC"
                     /locus_tag="OO357_000181"
     CDS             complement(55452..56261)
                     /gene="pabC"
                     /locus_tag="OO357_000181"
                     /EC_number="4.1.3.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate lyase"
                     /protein_id="HCR5885268.1"
                     /translation="MFLINGYKQESLAVSDRATQFGDGCFTTARVIDGKVSLLSAHIL
                     RLQDACQRLMISCDFWPQLEQEMKTLAAEQQNGVLKVVISRGSGGRGYSTLNSGPATR
                     ILSVTAYPAHYDRLRNEGMTLALSPVRLGRNPHLAGIKHLNRLEQVLIRSHLEQTNAD
                     EALVLDSEGWVTECCAANLFWRKGNVVYTPRLDQAGVNGIMRQFCIRLLAQSSYQLVE
                     VQASLEEALQADEMVICNALMPVMPVRACGDVTFSSATLYEYLAPLCERPN"
     gene            complement(56381..57622)
                     /gene="fabF"
                     /locus_tag="OO357_000182"
     CDS             complement(56381..57622)
                     /gene="fabF"
                     /locus_tag="OO357_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HCR5885269.1"
                     /translation="MSKRRVVVTGLGMLSPVGNTVESTWKALLAGQSGISLIDHFDTS
                     AYATKFAGLVKDFNCEDIISRKEQRKMDAFIQYGIVAGVQAMQDSGLEITEENATRIG
                     AAIGSGIGGLGLIEENHTSLMNGGPRKISPFFVPSTIVNMVAGHLTIMYGLRGPSISI
                     ATACTSGVHNIGHAARIIAYGDADVMVAGGAEKASTPLGVGGFGAARALSTRNDNPQA
                     ASRPWDKERDGFVLGDGAGMLVLEEYEHAKKRGAKIYAELVGFGMSSDAYHMTSPPEN
                     GAGAALAMANALRDAGIEASQIGYVNAHGTSTPAGDKAEAQAVKTIFGEAASRVLVSS
                     TKSMTGHLLGAAGAVESIYSILALRDQAVPPTINLDNPDEGCDLDFVPHEARQVSGME
                     YTLCNSFGFGGTNGSLIFKKI"
     gene            complement(57709..57945)
                     /gene="acpP"
                     /locus_tag="OO357_000183"
     CDS             complement(57709..57945)
                     /gene="acpP"
                     /locus_tag="OO357_000183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5885270.1"
                     /translation="MSTIEERVKKIIGEQLGVKQEEVTNNASFVEDLGADSLDTVELV
                     MALEEEFDTEIPDEEAEKITTVQAAIDYINGHQA"
     gene            complement(58156..58890)
                     /gene="fabG"
                     /locus_tag="OO357_000184"
     CDS             complement(58156..58890)
                     /gene="fabG"
                     /locus_tag="OO357_000184"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase FabG"
                     /protein_id="HCR5885271.1"
                     /translation="MNFEGKIALVTGASRGIGRAIAETLAARGAKVIGTATSENGAQA
                     ISDYLGANGKGLMLNVTDPASIESVLEKIRAEFGEVDILVNNAGITRDNLLMRMKDEE
                     WNDIIETNLSSVFRLSKAVMRAMMKKRHGRIITIGSVVGTMGNGGQANYAAAKAGLIG
                     FSKSLAREVASRGITVNVVAPGFIETDMTRALSDDQRAGILAQVPAGRLGGAQEIANA
                     VAFLASDEAAYITGETLHVNGGMYMV"
     gene            complement(58903..59832)
                     /gene="fabD"
                     /locus_tag="OO357_000185"
     CDS             complement(58903..59832)
                     /gene="fabD"
                     /locus_tag="OO357_000185"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HCR5885272.1"
                     /translation="MTQFAFVFPGQGSQTVGMLADMAASYPIVEETFAEASAALGYDL
                     WALTQQGSAEELNKTWQTQPALLTASVALYRVWQQQGGKAPAMMAGHSLGEYSALVCA
                     GVIDFADAVRLVEMRGKFMQEAVPEGTGAMAAIIGLDDAPIAKACEEAAEGQVVSPVN
                     FNSPGQVVIAGHKEAVERAGAACKAAGAKRALPLPVSVPSHCALMKPAADKLAVELAK
                     ITFNAPTVSVVNNVDVKCETNGDAIRDALVRQLYNPVQWTKSVEYMAAQGVEHLYEVG
                     PGKVLTGLTKRIVDTLTASALNEPSAMAAALEL"
     gene            complement(59848..60801)
                     /gene="fabH"
                     /locus_tag="OO357_000186"
     CDS             complement(59848..60801)
                     /gene="fabH"
                     /locus_tag="OO357_000186"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HCR5885273.1"
                     /translation="MYTKIIGTGSYLPEQVRTNADLEKMVDTSDEWIVTRTGIRERHI
                     AAPNETVSTMGFEAATRAIEMAGIEKDQIGLIVVATTSATHAFPSAACQIQSMLDIKG
                     CPAFDVAAACAGFTYALSVADQYVKSGAVKYALVVGSDVLARTCDPTDRGTIIIFGDG
                     AGAAVLAASEEPGIISTHLHADGSYGELLTLPNADRVNPENSIHLTMAGNEVFKVAVT
                     ELAHIVDETLAANNLDRSQLDWLVPHQANLRIISATAKKLGMSMDNVVVTLDRHGNTS
                     AASVPCALDEAVRDGRIKPGQLVLLEAFGGGFTWGSALVRF"
     gene            complement(60869..61939)
                     /gene="plsX"
                     /locus_tag="OO357_000187"
     CDS             complement(60869..61939)
                     /gene="plsX"
                     /locus_tag="OO357_000187"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HCR5885274.1"
                     /translation="MTRLTLALDVMGGDFGPSVTVPAALQALNSNSQLTLLLVGNPDA
                     ITPLLAKADFEQRSRLQIIPAQSVIASDARPSQAIRASRGSSMRMALELVKEGRAQAC
                     VSAGNTGALMGLAKLLLKPLEGIERPALVTVLPHQQKGKTVVLDLGANVDCDSTMLVQ
                     FAIMGSVLAEEVVEIPNPRVALLNIGEEEVKGLDSIRDASAVLKTIPSINYIGYLEAN
                     ELLTGKTDVLVCDGFTGNVTLKTMEGVVRMFLSLLKSQGEGKKRSWWLLLLKRWLQKS
                     LTRRFSHLNPDQYNGACLLGLRGTVIKSHGAANQRAFAVAIEQAVQAVQRQVPQRIAA
                     RLESVYPAGFELLDGGKSGTLR"
     gene            complement(62020..62193)
                     /gene="rpmF"
                     /locus_tag="OO357_000188"
     CDS             complement(62020..62193)
                     /gene="rpmF"
                     /locus_tag="OO357_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HCR5885275.1"
                     /translation="MAVQQNKPTRSKRGMRRSHDALTAVTSLSVDKTSGEKHLRHHIT
                     ADGYYRGRKVIAK"
     gene            complement(62245..62766)
                     /gene="yceD"
                     /locus_tag="OO357_000189"
     CDS             complement(62245..62766)
                     /gene="yceD"
                     /locus_tag="OO357_000189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001174481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA accumulation protein YceD"
                     /protein_id="HCR5885276.1"
                     /translation="MQKVKLPLTLDPVRTAQKRLDYQGIYTPDQVERVAESVVSVDSD
                     VECSMSFAIDNQRLAVLNGDAKVTVTLECQRCGKPFTHQVYTTYCFSPVRSDEQAEAL
                     PEAYEPIEVNEFGEIDLLAMVEDEIILALPVVPVHDSEHCEVSEADMVFGELPEEVQK
                     PNPFAVLASLKRK"
     gene            62965..63549
                     /gene="yceF"
                     /locus_tag="OO357_000190"
     CDS             62965..63549
                     /gene="yceF"
                     /locus_tag="OO357_000190"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415605.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-methyl-GTP pyrophosphatase"
                     /protein_id="HCR5885277.1"
                     /translation="MPKLILASTSPWRRALLEKLQISFECAAPEVDETPRSDESPRQL
                     VLRLAQEKSQSLASRYPDHLIIGSDQVCVLDGEITGKPLTEENARLQLRKASGNIVTF
                     YTGLALFNSANGHLQTEVEPFDVHFRHLSEAEIDNYVRKEHPLHCAGSFKSEGFGITL
                     FERLEGRDPNTLVGLPLIALCQMLRREGKNPLMG"
     gene            complement(63661..64620)
                     /gene="rluC"
                     /locus_tag="OO357_000191"
     CDS             complement(63661..64620)
                     /gene="rluC"
                     /locus_tag="OO357_000191"
                     /EC_number="5.4.99.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(955/2504/2580) synthase
                     RluC"
                     /protein_id="HCR5885278.1"
                     /translation="MKTETPSVKIVAITADEAGQRIDNFLRTQLKGVPKSMIYRILRK
                     GEVRVNKKRIKPEYKLEAGDEVRIPPVRVAEREEEAVSPHLQKVAALADVILYEDDHI
                     LVLNKPSGTAVHGGSGLSFGVIEGLRALRPEARFLELVHRLDRDTSGVLLVAKKRSAL
                     RSLHEQLREKGMQKDYLALVRGQWQSHVKSVQAPLLKNILQSGERIVRVSQEGKPSET
                     RFKVEERYAFATLVRCSPVTGRTHQIRVHTQYAGHPIAFDDRYGDREFDRQLTEAGTG
                     LNRLFLHAAALKFTHPGTGEVMRIEAPMDEGLKRCLQKLRNAR"
     gene            65193..68384
                     /gene="rne"
                     /locus_tag="OO357_000192"
     CDS             65193..68384
                     /gene="rne"
                     /locus_tag="OO357_000192"
                     /EC_number="3.1.26.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E"
                     /protein_id="HCR5885279.1"
                     /translation="MKRMLINATQQEELRVALVDGQRLYDLDIESPGHEQKKANIYKG
                     KITRIEPSLEAAFVDYGAERHGFLPLKEIAREYFPANYSAHGRPNIKDVLREGQEVIV
                     QIDKEERGNKGAALTTFISLAGSYLVLMPNNPRAGGISRRIEGDDRTELKEALASLEL
                     PEGMGLIVRTAGVGKSAEALQWDLSFRLKHWEAIKKAAESRPAPFLIHQESNVIVRAF
                     RDYLRQDIGEILIDNPKVLELARQHIAALGRPDFSSKIKLYTGEIPLFSHYQIESQIE
                     SAFQREVRLPSGGSIVIDSTEALTAIDINSARATRGGDIEETAFNTNLEAADEIARQL
                     RLRDLGGLIVIDFIDMTPVRHQRAVENRLREAVRQDRARIQISHISRFGLLEMSRQRL
                     SPSLGESSHHVCPRCSGTGTVRDNESLSLSILRLIEEEALKENTQEVHAIVPVPIASY
                     LLNEKRSAVNAIETRQDGVRCVIVPNDQMETPHYHVLRVRKGEETPTLSYMLPKLHEE
                     AMALPSEEEFAERKRPEQPALATFAMPDVPPAPTPAEPAATVVAPAPKAATATPAAPA
                     QPGLLSRFFGALKALFSGGEEAKPTEQPTPKAEAKPERQQDRRKPRQSNRRDRNERRD
                     TRSERTEGSDNREENRRNRRQAQQQTAETRESRQQAEVTEKARTTDEQQVPRRERSRR
                     RNDDKRQAQQEAKALNVEEQSVQETEQEERVRPVQPRRKQRQLNQKVRYEQSVAEEAV
                     VAPVVEETAAAEPIVQEAPAPRTELVKVPLPVVAQTAPEQQEENNADNRDNGGMPRRS
                     RRSPRHLRVSGQRRRRYRDERYPTQSPMPLTVACASPELASGKVWIRYPIVRPQDVQV
                     EEQREQEEVQVQPMVTEVPVAAAVEPVVSAPVVEEMAEVVEAPVPVAEPQPEVVETTH
                     PEVIAAAVTEQPQVITESDVAVAQEVAEHAEPMVEPQEETADADIEEVAETAEVVVAE
                     PEVVAQPAAPVVAEVAAEVETVAAVEPEITVEHNHATAPMTRAPAPEYVPEAPRHSDW
                     QRPTFAFEGKGAAGGHTATHHASAAPARPQPVE"
     gene            complement(68581..69523)
                     /gene="flgL"
                     /locus_tag="OO357_000193"
                     /pseudo
     CDS             complement(68581..69523)
                     /gene="flgL"
                     /locus_tag="OO357_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309488.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgL"
     gene            complement(69535..71177)
                     /gene="flgK"
                     /locus_tag="OO357_000194"
                     /pseudo
     CDS             complement(69535..71177)
                     /gene="flgK"
                     /locus_tag="OO357_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415600.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-associated protein FlgK"
     gene            complement(71243..72184)
                     /gene="flgJ"
                     /locus_tag="OO357_000195"
     CDS             complement(71243..72184)
                     /gene="flgJ"
                     /locus_tag="OO357_000195"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly peptidoglycan hydrolase FlgJ"
                     /protein_id="HCR5885280.1"
                     /translation="MISDSKLLASAAWDAQSLNELKAKASEDPAANIRPVARQVEGMF
                     VQMMLKSMRDALPKDGLFSSEHTRLYTSMYDQQIAQQMTAGKGLGLAEMMVKQMTPEQ
                     PLPEESTPAAPMKFPLETVVRYQNQTLSQLVQKAVPRNYDDSLPGDSRAFLAQLSLPA
                     QLASQQSGVPHHLILAQAALESGWGQRQIRRENGEPSYNLFGVKASGNWKGQVTEITT
                     TEYENGEAKKVKAKFRVYSSYLEALSDYVGLLTRNPRYAAVTTAASAEQGAQVLQDAG
                     YATDPHYARKLTNMIQQMKSISDKVSKTYSMNIDNLF"
     gene            complement(72184..73281)
                     /gene="flgI"
                     /locus_tag="OO357_000196"
     CDS             complement(72184..73281)
                     /gene="flgI"
                     /locus_tag="OO357_000196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415598.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring protein FlgI"
                     /protein_id="HCR5885281.1"
                     /translation="MIKFLSALILLLVTTAAQAERIRDLTSVQGVRQNSLIGYGLVVG
                     LDGTGDQTTQTPFTTQTLNNMLSQLGITVPTGTNMQLKNVAAVMVTASLPPFGRQGQT
                     IDVVVSSMGNAKSLRGGTLLMTPLKGVDSQVYALAQGNILVGGAGASAGGSSVQVNQL
                     NGGRITNGAVIERELPSQFGVGNTLNLQLNDEDFSMAQQIADTINRVRGYGSATALDA
                     RTIQVRVPSGNSSQVRFLADIQNMQVNVTPQDAKVVINSRTGSVVMNREVTLDSCAVA
                     QGNLSVTVNRQANVSQPDTPFGGGQTVVTPQTQIDLRQSGGSLQSVRSSASLNNVVRA
                     LNALGATPMDLMSILQSMQSAGCLRAKLEII"
     gene            complement(73293..73991)
                     /gene="flgH"
                     /locus_tag="OO357_000197"
     CDS             complement(73293..73991)
                     /gene="flgH"
                     /locus_tag="OO357_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body L-ring protein FlgH"
                     /protein_id="HCR5885282.1"
                     /translation="MQKNAAHTYAISSLLVLSLTGCAWIPSTPLVQGATSAQPVPGPT
                     PVANGSIFQSAQPINYGYQPLFEDRRPRNIGDTLTIVLQENVSASKSSSANASRDGKT
                     NFGFDTVPRYLQGLFGNARADVEASGGNTFNGKGGANASNTFSGTLTVTVDQVLVNGN
                     LHVVGEKQIAINQGTEFIRFSGVVNPRTISGSNTVPSTQVADARIEYVGNGYINEAQN
                     MGWLQRFFLNLSPM"
     gene            complement(74044..74826)
                     /gene="flgG"
                     /locus_tag="OO357_000198"
     CDS             complement(74044..74826)
                     /gene="flgG"
                     /locus_tag="OO357_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgG"
                     /protein_id="HCR5885283.1"
                     /translation="MISSLWIAKTGLDAQQTNMDVIANNLANVSTNGFKRQRAVFEDL
                     LYQTIRQPGAQSSEQTTLPSGLQIGTGVRPVATERLHSQGNLSQTNNSKDVAIKGQGF
                     FQVMLPDGSSAYIRDGSFQVDQNGQLVTAGGFQVQPAITIPANALSITIGRDGVVSVT
                     QQGQAAPVQVGQLNLTTFMNDTGLESIGENLYTETQSSGAPNESTPGLNGAGLLYQGY
                     VETSNVNVAEELVNMIQVQRAYEINSKAVSTTDQMLQKLTQL"
     gene            complement(74964..75715)
                     /gene="flgF"
                     /locus_tag="OO357_000199"
                     /pseudo
     CDS             complement(74964..75715)
                     /gene="flgF"
                     /locus_tag="OO357_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415595.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
     gene            complement(75735..76874)
                     /locus_tag="OO357_000200"
     CDS             complement(75735..76874)
                     /locus_tag="OO357_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook protein FlgE"
                     /protein_id="HCR5885284.1"
                     /translation="MAFSQAVSGLNAAATNLDVIGNNIANSATYGFKSGTASFADMFA
                     GSKVGLGVKVAGITQDFTDGTTTNTGRGLDVAISQNGFFRLVDSNGSVFYSRNGQFKL
                     DENRNLLNTQGLQLTGYPVTGTPPTIQQGANPTNISIPNTLMAAKTTTTASMQINLNS
                     SDPLPTVTPFSASNADSYNKKGSVTVFDSQGNAHDMSVYFVKTGDNNWQVYTQDSSDP
                     NSIAKTATTLEFNANGTLVDGAMANNIATGANNIVATTQNGYKPGDLVSYQINDDGTV
                     VGNNSNEQTQLLGQIVLANFANNEGLASEGDNVWSATQSSGVALLGTAGTGNFGTLTN
                     GALEASNVDLSKELVNMIVAQRNYKSNAQTIKTQDQILNTRVNLR"
     gene            complement(76899..77594)
                     /gene="flgD"
                     /locus_tag="OO357_000201"
     CDS             complement(76899..77594)
                     /gene="flgD"
                     /locus_tag="OO357_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HCR5885285.1"
                     /translation="MSIAVTTTDPTNTGVSTTSSSSLTGSNAADLQSSFLTLLVAQLK
                     NQDPTNPMENNELTSQLAQISTVSGIEKLNTTLGSISGQIDNSQSLQASNLIGHGVMI
                     PGTTVLAGTGSEEGAVTTTTPFGVELQQAADKVTATITDKNGAVVRTIDIGELTAGVH
                     SFTWDGSLTDGSTAPNGSYNVAISASNGGTQLVAQPLQFALVQGVIRGNSGNTLDLGT
                     YGTTTLDEVRQII"
     gene            complement(77606..78009)
                     /gene="flgC"
                     /locus_tag="OO357_000202"
                     /pseudo
     CDS             complement(77606..78009)
                     /gene="flgC"
                     /locus_tag="OO357_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740734.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgC"
     gene            complement(78013..78429)
                     /gene="flgB"
                     /locus_tag="OO357_000203"
     CDS             complement(78013..78429)
                     /gene="flgB"
                     /locus_tag="OO357_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body rod protein FlgB"
                     /protein_id="HCR5885286.1"
                     /translation="MLDKLDAALRFQQEALNLRAQRQEVLAANIANADTPGYQARDID
                     FASELKKVMQRGRDATSVVALTMTSTQHIPAQALTPPTAELQYRIPDQPSLDGNTVDM
                     DRERTQFADNSLQYQMSLSALSGQIKGMMNVLQSGN"
     gene            78584..79243
                     /gene="flgA"
                     /locus_tag="OO357_000204"
     CDS             78584..79243
                     /gene="flgA"
                     /locus_tag="OO357_000204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body P-ring formation protein
                     FlgA"
                     /protein_id="HCR5885287.1"
                     /translation="MLTIKRSVAIIAILFSPLSTASNLTSQLHNFFSAQLAGVSDEVR
                     VSIRTAPNLLPPCEQPLLSMSNNSRLWGNVNVLARCGNDKRYLQVNVQATGNYVVAAM
                     PIVRGGKLEAGNVKLKRGRLDTLPPRTVLDINQLVDAVSLRDLSPDQPIQLTQFRQAW
                     RVKAGQRVNVIASGDGFSANAEGQALNNAAVAQNARVRMVSEQVVSGVVDADGNILIN
                     L"
     gene            79319..79612
                     /gene="flgM"
                     /locus_tag="OO357_000205"
     CDS             79319..79612
                     /gene="flgM"
                     /locus_tag="OO357_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-28 factor FlgM"
                     /protein_id="HCR5885288.1"
                     /translation="MSIDRTSPLKPVSTVQPRETTDAPVTNSRAAKTTASTSTSVTLS
                     DAQAKLMQPGSSDINLERVEALKLAIRNGELKMDTGKIADALINEAQQDLQSN"
     gene            79617..80033
                     /gene="flgN"
                     /locus_tag="OO357_000206"
     CDS             79617..80033
                     /gene="flgN"
                     /locus_tag="OO357_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FlgN"
                     /protein_id="HCR5885289.1"
                     /translation="MTRLAEILDQMSAVLNDLKTVMDQEQQHLSMGQINGSQLQWITE
                     QKSSLLATLDYLEQLRKKEPNTANSVDISQRWQEITVKTQQLRQMNQHNGWLLEGQIE
                     RNQQALEMLKPHQEPTLYGANGQTSTPHRGSKKISI"
     gene            complement(80073..81608)
                     /gene="murJ"
                     /locus_tag="OO357_000207"
     CDS             complement(80073..81608)
                     /gene="murJ"
                     /locus_tag="OO357_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706990.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HCR5885290.1"
                     /translation="MNLLKSLAAVSSMTMFSRVLGFARDAIVARIFGAGMATDAFFVA
                     FKLPNLLRRIFAEGAFSQAFVPILAEYKSKQGEDATRVFVSYVSGLLTLALAVVTVAG
                     MLAAPWVIMVTAPGFADTADKFALTSQLLKITFPYILLISLASLVGAILNTWNRFSIP
                     AFAPTLLNISMIGFALFAAPYFNPPVLALAWAVTVGGILQLVYQLPHLKKIGMLVLPR
                     INFHDAGAMRVVKQMGPAILGVSVSQISLIINTIFASFLASGSVSWMYYADRLMEFPS
                     GVLGVALGTILLPSLSKSFASGNHDEYNRLMDWGLRLCFLLALPSAVALGILSGPLTV
                     SLFQYGKFTAFDALMTQRALIAYSVGLIGLIVVKVLAPGFYSRQDIKTPVKIAIVTLI
                     LTQLMNLAFIGPLKHAGLSLSIGLAACLNASLLYWQLRKQKIFTPQPGWMAFLLRLVV
                     AVLVMSGVLLGMLHIMPEWSLGTMPWRLLRLMAVVLAGIAAYFAALAVLGFKVKEFAR
                     RTV"
     gene            complement(81808..82641)
                     /locus_tag="OO357_000208"
     CDS             complement(81808..82641)
                     /locus_tag="OO357_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000736138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5885291.1"
                     /translation="MKKLRIGVVGLGGIAQKAWLPVLAAASDWTLQGAWSPTRAKALP
                     ICDSWRIPYADSLSSLAASCDAVFVHSSTASHFDVVSTLLNAGVHVCVDKPLAENLCD
                     AERLVELAARKKLTLMVGFNRRFAPLYGELKTQLATAASLRMDKHRSNSVGPHDLYFT
                     LLDDYLHVVDTALWLSGGKASLDGGTLLTNDAGEMLFAEHHFSAGPLQITTCMHRRAG
                     SQRETVQAVTDGALIDITDMREWREERGQGVVNKPIPGWQSTLEQRGFVGCARHFIEC
                     V"
     gene            complement(82643..83290)
                     /locus_tag="OO357_000209"
     CDS             complement(82643..83290)
                     /locus_tag="OO357_000209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceH family protein"
                     /protein_id="HCR5885292.1"
                     /translation="MKYQLTALEARVIGCLLEKQVTTPEQYPLSVNGVVTACNQKTNR
                     EPVMNLSESEVQEQLDNLVKRHYLRTVSGFGNRVTKYEQRFCNSEFGDLKLSAAEVAL
                     ITTLLLRGAQTPGELRSRAARMYEFSDMAEVESTLEQLANREDGPFVVRLAREPGKRE
                     SRYMHLFSGEVEDQPAVTAMSNMVDGDLQTRVEALEIEVAELKQRLDSLLAHLGD"
     gene            complement(83301..83885)
                     /gene="rimJ"
                     /locus_tag="OO357_000210"
     CDS             complement(83301..83885)
                     /gene="rimJ"
                     /locus_tag="OO357_000210"
                     /EC_number="2.3.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S5-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5885293.1"
                     /translation="MFGYRSNVPKVRLTTDRLVVRLVHDRDAWRLADYYAENRHFLKP
                     WEPVRDESHCYPSGWQARLGMINEFHKQGSAFYFGLFDPDEKEIIGVANFSNVVRGSF
                     HACYLGYSIGQKWQGKGLMFEALTAAIRYMQRTQHIHRIMANYMPHNKRSGDLLARLG
                     FEKEGYAKDYLLIDGQWRDHVLTALTTPDWTPGR"
     gene            84121..85329
                     /gene="mdtH"
                     /locus_tag="OO357_000211"
     CDS             84121..85329
                     /gene="mdtH"
                     /locus_tag="OO357_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtH"
                     /protein_id="HCR5885294.1"
                     /translation="MSRVSQARNLGKYFLLIDNMLVVLGFFVVFPLISIRFVDQMGWA
                     AVMVGIALGLRQFIQQGLGIFGGAIADRFGAKPMIVTGMLMRAAGFATMGIAHEPWLL
                     WFSCLLSGLGGTLFDPPRSALVVKLIRPQQRGRFFSLLMMQDSASAVIGALLGSWLLQ
                     YDFRLVCATGAVLFVLCAAFNAWLLPAWKLSTVRTPVREGMTRVMRDKRFVTYVLTLA
                     GYYMLAVQVMLMLPIMVNDVAGAPSAVKWMYAIEACLSLTLLYPIARWSEKHFRLEHR
                     LMAGLLIMSLSMMPVGMVSGLQQLFTLICLFYIGSIIAEPARETLSASLADARARGSY
                     MGFSRLGLAIGGAIGYIGGGWLFDLGKSAHQPELPWMMLGIIGIFTFLALGWQFSQKR
                     AARRLLERDA"
     gene            85393..86040
                     /gene="grxB"
                     /locus_tag="OO357_000212"
     CDS             85393..86040
                     /gene="grxB"
                     /locus_tag="OO357_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 2"
                     /protein_id="HCR5885295.1"
                     /translation="MKLYIYDHCPYCLKARMIFGLKNIPVELHVLLNDDAETPTRMVG
                     QKQVPILQKDDSRYMPESMDIVHYVDKLDGKPLLTGKRSPAIEEWLRKVNGYVNKLLL
                     PRFAKSAFDEFSTPAARKYFVDKKEASAGNFADLLAHSDGLIKNISDDLRALDKLIVK
                     PNAVNGELSEDDIQLFPLLRNLTLVAGINWPSRVANYRDNMAKQTQINLLSSMAI"
     gene            86174..86734
                     /locus_tag="OO357_000213"
     CDS             86174..86734
                     /locus_tag="OO357_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5885296.1"
                     /translation="MNKFLFAAALIVSGLLVGCNQLTQYTITEQEINQSLAKHNNFSK
                     DIGLPGVADAHIVLTNLTSQIGREEPNKVTLTGDANLDMNSLFGSQKATMKLKLKALP
                     VFDKEKGAIFLKEMEVVDATVQPEKMQTVMQTLLPYLNQALRNYFNQQPAYVLREDGS
                     QGEAMAKKLAKGIEVKPGEIVIPFTD"
     gene            86840..87886
                     /gene="pyrC"
                     /locus_tag="OO357_000214"
     CDS             86840..87886
                     /gene="pyrC"
                     /locus_tag="OO357_000214"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HCR5885297.1"
                     /translation="MTAPSQVLKIRRPDDWHLHLRDGDMLKTVVPYTSEIYGRAIVMP
                     NLAPPVTTVEAAVAYRQRILDAVPAGHDFTPLMTCYLTDSLDPNELERGFNEAVFTAA
                     KLYPANATTNSSHGVTSIDAIMPVLERMEKIGMPLLVHGEVTHADIDIFDREARFIES
                     VMEPLRQRLTALKVVFEHITTKDAADYVRDGNERLAATITPQHLMFNRNHMLVGGVRP
                     HLYCLPILKRNIHQQALRELVASGFNRVFLGTDSAPHARHRKESSCGCAGCFNAPTAL
                     GSYATVFEEMNALQHFEAFCSVNGPQFYGLPVNDTFIELVREEQQVAESIALTDDTLV
                     PFLAGETVRWSVKQ"
     gene            87960..88205
                     /gene="dinI"
                     /locus_tag="OO357_000215"
     CDS             87960..88205
                     /gene="dinI"
                     /locus_tag="OO357_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein I"
                     /protein_id="HCR5885298.1"
                     /translation="MRIEVTIAKTSPLPAGAIDALAGELSRRIQYAFPDNEGHVSVRY
                     AAANNLSVIGATKEDKQRISEILQETWESADDWFVSE"
     gene            88495..88749
                     /gene="bssS"
                     /locus_tag="OO357_000216"
     CDS             88495..88749
                     /gene="bssS"
                     /locus_tag="OO357_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309465.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssS"
                     /protein_id="HCR5885299.1"
                     /translation="MEKNNEVIQTHPLVGWDISTVDSYDALMLRLHYQTPNKSEQEGT
                     EVGQTLWLTTDVARQFISILEAGIAKIESGDFPVNEYRRH"
     gene            88864..89982
                     /gene="solA"
                     /locus_tag="OO357_000217"
     CDS             88864..89982
                     /gene="solA"
                     /locus_tag="OO357_000217"
                     /EC_number="1.5.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-methyl-L-tryptophan oxidase"
                     /protein_id="HCR5885300.1"
                     /translation="MKYDLIIIGSGSVGAAAGYYATRAGLNVLMTDAHMPPHQHGSHH
                     GDTRLIRHAYGEGEKYVPLVLRAQTLWDELSRHNEDDPIFVRSGVINLGPADSAFLAN
                     VAHSAEQWQLNVEKLDAQGIMARWPEIRVPDNYIGLFETDSGFLRSELAIKTWIQLAK
                     EAGCAQLFNCPVTAIRHDDDGVTIETVDGEYQAKKAIVCAGTWVKDLLPELPVQPVRK
                     VFAWYQADGRYSVKNKFPAFTGELPNGDQYYGFPAENDALKIGKHNGGQVIHSADERV
                     PFAEVASDGSEAFPFLRNVLPGIGCCLYGAACTYDNSPDEDFIIDTLPGHDNTLLITG
                     LSGHGFKFASVLGEIAADFAQDQKSDFDLTPFRLSRFQ"
     gene            90030..90143
                     /locus_tag="OO357_000218"
     CDS             90030..90143
                     /locus_tag="OO357_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceO family protein"
                     /protein_id="HCR5885301.1"
                     /translation="MRRLLHYLINNIREHLMLYLFLWGLLAIMDLIYVFYF"
     gene            90404..90970
                     /locus_tag="OO357_000219"
     CDS             90404..90970
                     /locus_tag="OO357_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706979.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HCR5885302.1"
                     /translation="MSFTNTPERYGVISAAFHWLSAIIVYGMFALGLWMVTLSYYDGW
                     YHKAPELHKSIGILLMMGLVIRVLWRVISPPPGPLPSYSPMTRLAAKAGHLALCLLLF
                     AIGISGYLISTADGKPISVFGWFDVPATLADAGAQADFAGALHFWLAWSVVVLSVMHG
                     FMALKHHFIDKDDTLKRMLGKSSSDYGV"
     gene            90974..91549
                     /locus_tag="OO357_000220"
     CDS             90974..91549
                     /locus_tag="OO357_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HCR5885303.1"
                     /translation="MKKSLLGLTFASLMFSAGSAVAADYKIDKEGQHAFVNFRIQHLG
                     YSWLYGTFKDFDGTFTFDEKNPAADKVNVTINTTSVDTNHAERDKHLRSADFLNTTKY
                     PQATFTSTSVKKDGDELDITGDLTLNGVTKPVTLEAKLIGQGDDPWGGKRAGFEAEGK
                     IKLKDFNIKTDLGPASQEVDLIISVEGVQQK"
     gene            complement(91591..92636)
                     /locus_tag="OO357_000221"
                     /pseudo
     CDS             complement(91591..92636)
                     /locus_tag="OO357_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706977.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
     gene            92861..93781
                     /gene="lpxL"
                     /locus_tag="OO357_000222"
     CDS             92861..93781
                     /gene="lpxL"
                     /locus_tag="OO357_000222"
                     /EC_number="2.3.1.241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid IV(A) lauroyltransferase"
                     /protein_id="HCR5885304.1"
                     /translation="MTNLPKFSTALLHPRYWLTWLGIGVLWLVVQLPYPIIYRLGCGL
                     GKLALRFMKRRAKIVHRNLELCFPEMSEQERRKMVVKNFESVGMGLMETGMAWFWPDR
                     RIARWTEVIGMEHIRDVQAQKRGILLVGIHFLTLELGARQFGMQEPGIGVYRPNDNPL
                     IDWLQTWGRLRSNKSMLDRKDLKGMIKALKKGEVVWYAPDHDYGPRSSVFVPLFAVEQ
                     AATTTGTWMLARMSGACLVPFVPRRKPDGKGYQLIMLPPECSPPLDDAETTAAWMNKV
                     VEKCIMMAPEQYMWLHRRFKTRPEGVPSRY"
     gene            93953..94150
                     /locus_tag="OO357_000223"
     CDS             93953..94150
                     /locus_tag="OO357_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000074158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter"
                     /protein_id="HCR5885305.1"
                     /translation="MSPCENDTPINWKRNLIVAWLGCFLTGAAFSLVMPFLPLYVEQL
                     GVTGHSALNMWSGIVFSIMGS"
     gene            94174..>95074
                     /locus_tag="OO357_000224"
     CDS             94174..>95074
                     /locus_tag="OO357_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001542244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5885306.1"
                     /translation="MRRAISYIRFSSERQLKGDSVRRQSKLVTDWLDKNPEFYLDSSL
                     SFKDLGKSAFSGKHLKGGLGDFLTAIEKGLVKAGDTLLIESLDRLSRQDIDIASELLR
                     RILRAGVDVVTLSDGEHYTRESLKDPLALIKSILIMQRAHEESLRKSERVQAAWNRKK
                     ELISEGIKVSRRCPAWLRLNDDRRTFTIIPDKVEVVKRAFDLRLQGLSFWAITRTLND
                     EGHLSLNQYTPKQKGWSDTAVKKLLRNRAVIGCFTPAGREEVQGYYPAIISESLFYRV
                     QQLNTGQYGRASVSSNPLSVNLFR"
BASE COUNT        22879 a        25206 c        23469 g        23520 t
ORIGIN      
        1 tatgctcgat atgctattaa taatatccac ataaataaaa caacgggcgt gttatacgcc
       61 cgtttaaata tttaacacat gtagtgatta catattcttg atgatcgcat caccaaactc
      121 tgaacatttc agcagtttag cgccttccat cagacgctcg aagtcatagg ttacggtctt
      181 ggcgttgatc gcgccttcca tacctttaac aatcaggtct gcggcttcag tccagcccat
      241 atggcgtaac atcatctcag cggagagaat aatagagcca gggtttactt tgtcctgacc
      301 ggcgtatttc ggcgcagtac cgtgggtggc ttcaaacagg gcgcattcgt caccgatgtt
      361 tgctccaggg gcgataccga taccgccaac ctgcgctgcc agagcgtcag aaatgtagtc
      421 accgttcagg ttcatacagg cgataacatc atattcagcc ggacgcagca ggatctgttg
      481 caggaatgca tcagcaatca cgtctttaat gacgatctct ttgccggtat tcgggttttt
      541 aactttcagc cacgggccgc cgtcgatcag ttcaccgcca aattcttcac gcgccagctg
      601 gtagccccag tctttaaacg cgccttcggt gaacttcatg atgttgcctt tgtgcaccag
      661 ggtcacagag tcacgatcgt tagcaattgc gtattcgatc gctgcacgaa ccagacgttt
      721 ggtgccttct tcagaacacg gcttaatacc gataccgcaa tgttccggga agcgaatttt
      781 cttcacgccc atctcttcac gcaggaattt aatcactttc tcggcgtcgg cagagtcagc
      841 tttccattcg atacccgcat aaatgtcttc cgagttttca cggaagataa ccatatcggt
      901 cagttcaggg tgtttaaccg ggcttggagt gccctgatag taacgtaccg gacgcaggca
      961 gatgtagaga tccagttcct gacgcagggc aacgttgaga gaacgaatac cgccaccaac
     1021 cggagtggtc agcgggcctt taatggcaac gcgatattca cgaatcagat caagggtttc
     1081 agcaggcagc cagacatcct gaccataaac ctgtgtggat ttttcaccgg tgtaaatttc
     1141 catccaggag attttacgct cgcctttata ggctttctcg actgcagcgt cgaccacttt
     1201 cagcatggct ggggttacat ctacaccgat tccatcacct tcaatgtaag ggataatcgg
     1261 attttcagga acgttgagtt tgccgttttg cagggtgatc ttcttgcctt gtgccggaac
     1321 aactacttta ctttccattc acctctcctt cgagcgctac tggtttgctc gtctgccacc
     1381 acgttgcata tgcgtttgcg tcctgcgata cggatgcttt agagcaattt tttgttaatg
     1441 atttgtaatt gacttgtcaa tactacccta ttgtttgccg tcatgaaaga tgcgcgttat
     1501 taggctataa tgcggcaatt cataatctct gaaaatacca tgcaaaaaac ttcttttaga
     1561 aatcaccagg ttaagcgatt cagctcgcaa cgttctacca ggcgtaaaca tgaaaaccag
     1621 cccacgcgtg tgatcctctt caataaaccc tacgatgttc ttccgcagtt caccgatgaa
     1681 accggacgca aaacattaaa agaattcatc ccggttcagg gcgtttatgc agcaggtcgc
     1741 cttgaccgcg atagcgaagg gttgctggtg ctgaccaata acggcgcgtt gcaggcgcgt
     1801 ttaacccagc cgggtaaacg caccggaaaa atctattatg tgcaggtgga aggtattccc
     1861 acacaagacg cacttgaagc cttgcgcaat ggcgtaacct taaatgatgg ccctaccctg
     1921 cccgcaggcg cggagctggt tgacgaaccc gcgtggttat ggccgcggaa tccaccaatt
     1981 cgtgaacgca aaagtattcc caccagctgg ctgaagatca ccttatatga aggacgtaat
     2041 cgccaggtgc gccgcatgac cgcccatgtt ggtttcccca cgctgcgact gattcgctat
     2101 gcgatgggag attactcttt ggataatctt gccaatggcg aatggcgaga agtgacagat
     2161 taaggatgca aaatgtttaa accgcacgtt accgttgctt gcgtggtgca cgcagaaggc
     2221 aaatttttag tcgttgaaga gacgattaat ggtaaagcgt tatggaacca acctgccggg
     2281 catctggaag ccgatgaaac cttagtggaa gccgccgccc gtgaactgtg ggaagaaacc
     2341 ggcatcagcg cgcagccgca acactttatt cgtatgcatc agtggattgc gccagataaa
     2401 acgccgtttt tgcgtttcct ctttgccatt gagcttgagc aaatatgccc gacccagcct
     2461 catgacagcg atatcgactg ctgccgttgg gtcagcgccg aagaaatttt acaggcgtca
     2521 aatcttcgtt cgccgctggt ggcggaaagt attcgttgtt atcaaagcgg gcaacgttat
     2581 ccgctggaga tgattggcga ttttaactgg ccttttacaa agggtgtcat ctaaaaggct
     2641 gcgtgataga atacgccgcc ttgaagttca atgtcgtgag tgatccaatg tctgaaaccg
     2701 caaaaaaagt aatcgtcggc atgtccggcg gtgtcgattc ctccgtttct gcctggctgt
     2761 tgcaacaaca gggatatcag gtcgaaggcc tgtttatgaa gaactgggaa gaagacgacg
     2821 gtgaggaata ttgcacagca gcagcggatc tggctgatgc ccaggcggtc tgcgacaagc
     2881 tcggcattga actgcacacc gttaactttg ctgccgagta ctgggacaac gtcttcgaac
     2941 tgttccttgc cgaatataaa gccggtcgca cgccgaatcc ggatattctg tgcaacaagg
     3001 agatcaaatt taaagccttc ctcgaatttg ccgccgaaga tttaggtgcc gattatatcg
     3061 ctaccggtca ttacgtacgt cgggccgatg tcgatggcaa gagccgcctg ctgcgtggtc
     3121 tggacagcaa taaagaccag agctacttcc tttatacgct cagccatgag cagattgcgc
     3181 aaagcctgtt cccggtaggc gaactggaaa aaccgcaggt gcgtaagatt gctgaagatc
     3241 ttggtctggt caccgcgaag aaaaaagact ctaccggcat ttgcttcatt ggcgaacgta
     3301 aattccgcga gttcctgggc cgttatctcc cggcgcaacc gggcaaaatc attaccgtcg
     3361 atggcgatga aattggtgag caccaggggc tgatgtatca cactctcggt cagcgtaaag
     3421 gtctgggtat cggtggcacc aaagaaggta ccgaagaacc gtggtatgtg gtggacaaag
     3481 acgtcgaaaa caacattctg gttgtcgctc agggccatga acacccgcgg ctgatgtctg
     3541 tcgggttgat tgcccagcag ctgcactggg tcgatcgcga accattcacc ggcactatgc
     3601 gttgcacggt aaaaacccgc tatcgccaga ccgacattcc gtgcaccgtc aaggcgctgg
     3661 acgacgatcg cattaaagtg attttcgatg agccggttgc cgccgtgacg ccgggccagt
     3721 ctgccgtctt ctataacggt gaagtgtgcc tcggtggcgg tattattgag cagcgtctgc
     3781 cgctgccggt ctgattatta tctttactta aaacaaggaa gcagtgaacg tggcaaagaa
     3841 ttactatgac atcaccctcg ccctggccgg tatttgtcag tcggcacgcc tggtgcaaca
     3901 actcgctcac caggggcatt gtgatgccga tgcgctacac gtctcactca acagtattat
     3961 tgatatgaac cccagctcga cgctggcggt ttttggcggt agcgaagcca acctgcgcgt
     4021 cgggctggaa accctgctcg gcgtgctcaa tgccagcagt tgccagggct taaacgccga
     4081 attaacccgc tacacactca gcttgatggt gcttgagcgc aaactctcct cagcgaaagg
     4141 cgcgctcgac actctgggca accggatcaa cggcctgcaa cgccagctcg aacacttcga
     4201 tttacagtcc gaaacgctga tgagcgcgat ggctgctatc tatgttgatg tgatcagccc
     4261 gcttggcccg cgcattcagg tcaccggttc ccctgctgta ctgcaaagcc cacaagtgca
     4321 ggcgaaagtt cgcgcaaccc tgctggcagg cattcgcgcc gccgtgctct ggcaccaggt
     4381 cggcggcgga cgtctacaac tgatgttttc tcgtaatcgc ctgaccactc aggcaaaaca
     4441 aattcttgct catttaaccc cggagttgtg atctatggaa ttatcctcac tgaccgccgt
     4501 ttcccctgtc gatggacgct acggcgataa agtcagcgcg ctgcgcggga ttttcagcga
     4561 atatggtttg ctgaaattcc gtgtacaagt tgaagtacgt tggctgcaaa aactggccgc
     4621 gcacgcagcg atcaaggaag ttcctgcttt tgctgccgac gcaatcggtt accttgatgc
     4681 aattgtcgcc aatttcagcg aagaagatgc cgcacgcatc aaaaccatcg agcgtaccac
     4741 taaccacgac gttaaagcgg ttgagtattt cctgaaagaa aaagtggcgg agatcccgga
     4801 actgcacgcg gtttctgaat tcatccactt tgcctgtact tcggaagata tcaataacct
     4861 ctcccacgca ttaatgctga aaaccgcgcg tgatgaagtg atcctgccgt actggcgtca
     4921 actgattgat ggcattaaag atctcgccgt tcagtaccgc gatatcccgc tgctgtctcg
     4981 tacccacggt cagccagcca cgccgtcaac catcggtaaa gagatggcaa acgtcgccta
     5041 ccgtatggag cgccagcacc gccagcttaa ccaggtggag atcctcggca aaatcaacgg
     5101 cgcggtcggt aactataacg cccacatcgc cgcttacccg gaagttgact ggcatcagtt
     5161 cagcgaagag ttcgtcacct cgctgggtat tcaatggaac ccgtacacta cccagattga
     5221 accgcacgac tacattgccg aactgtttga ttgcgttgcg cgcttcaaca ccattctgat
     5281 cgactttgac cgtgacgtct ggggttatat cgcccttaac cacttcaaac agaaaaccat
     5341 tgctggtgag attggttctt ccaccatgcc gcataaagtt aacccgatcg acttcgaaaa
     5401 ctccgaaggg aatctgggcc tttccaacgc ggtattgcag catctggcaa gcaaactgcc
     5461 ggtttcccgc tggcagcgtg acctgaccga ctccaccgtg ctgcgtaacc tcggcgtggg
     5521 tatcggttat gcgctgattg cgtatcaatc caccctgaaa ggcgtgagca aactggaagt
     5581 gaaccgtgac catctgctgg atgagctgga tcacaactgg gaagtgctgg cagaaccaat
     5641 ccagacagtt atgcgtcgct atggcatcga aaaaccgtac gagaagctga aagagcttac
     5701 tcgcggtaag cgcgttgacg ccgaaggcat gaagcagttt atcgatggtc tggcgttgcc
     5761 agaagaagag aaagcccgcc tgaaagcgat gacgccggct aactatattg gtcgagctat
     5821 cacgatggtt gatgagctga aataagcctc aaatcagtgc cggatggcga tgctgtccgg
     5881 cctgcttatt aagattatcc actttttctt ttttcacttt acctcccctc cccgctggtt
     5941 tatttaatgt ttacccccat aaacacataa tcgcgttaca ctattttaat aattaagaca
     6001 gggagaaata aaaatgcgcg tactggttgt tgaagacaat gcgttgttac gtcaccacct
     6061 taaagttcag attcaggatg ctggtcatca ggtcgatgac gcagaagatg ccaaagaagc
     6121 cgattattat ctcaatgaac atttaccgga tattgcgatt gtcgatctcg gattgcctga
     6181 cgaggacggt ctgtcactga ttcgccgctg gcgtagcaac gatgtttcac tgccgattct
     6241 ggtattaacc gcccgtgaaa gctggcagga caaagtcgaa gtattaagtg ccggtgctga
     6301 tgattacgtg actaaaccgt ttcatattga agaggtgatg gcgcgaatgc aggcattaat
     6361 gcggcgtaat agcggtctgg cttcacaggt catttcgctc cccccgtttc aggttgatct
     6421 ctctcgccgt gaattatcta ttaatgacga agtgatcaaa ctgaccgcgt tcgaatacac
     6481 cattatggaa acgttgatac gcaataatgg caaagtggtc agcaaagatt cgttaatgct
     6541 acaactctat ccggatgcgg agctgcggga aagccatacc attgatgtac tgatgggacg
     6601 tctgcgcaaa aaaattcagg cacaatatcc gcaagaagtc attaccaccg ttcgcggcca
     6661 gggctacctg ttcgaattgc gctgatgaaa aaattactgc gtcttttttt cccgctctcg
     6721 ctgcgggtac gttttctgtt ggcaacggca gcggtagtac tggtgctttc gcttgcctac
     6781 ggaatggtcg cgctgatcgg ttatagcgtc agtttcgata aaactacgtt tcggctgtta
     6841 cgtggcgaga gcaatctgtt ctataccctt gcgcagtggg aaaacaataa gttgcatgtc
     6901 gagttacccg aaaatatcga caagcaaagc cccaccatga cgctaattta tgatgagaac
     6961 gggcagcttt tatgggcgca acgtgacgtg ccctggctga tgaaaatgat ccagcctgac
     7021 tggctgaaat cgaatggttt tcatgaaatt gaagcggatg ttaacgatac cagcctcttg
     7081 ctgagtggag atcattcgat acagcaacag ttgcaggaag tgcgggaaga tgatgacgac
     7141 gcggagatga cccactcggt ggcggtaaac gtctacccgg caacatcgcg gatgccaaaa
     7201 ttaaccattg tggtggtgga taccattccg gtggagctaa aaagttccta tatggtctgg
     7261 agctggttta tctatgtgct ctcagccaat ctgctgttag tgatcccgct gctgtgggtc
     7321 gccgcctggt ggagtttacg ccccatcgaa gccctggcaa aagaagtccg cgaactggaa
     7381 gaacataacc gcgaattgct caatccagcc acaacgcgag aactgaccag tctggtacga
     7441 aacctgaacc gattgttaaa aagtgaacgc gaacgttacg acaaataccg tacgacgctc
     7501 accgacctga cccatagtct gaaaacgcca ctggcggtgc tgcaaagtac gctgcgttct
     7561 ctgcgtagtg aaaagatgag cgtcagtgat gctgagccgg taatgctgga gcaaatcagc
     7621 cgcatttcac agcaaattgg ctactacctg catcgtgcca gtatgcgcgg cgggacattg
     7681 ctcagccgcg agctgcatcc ggtcgcccca ctgctggaca atctcacctc agcgctgaac
     7741 aaagtgtatc aacgcaaagg ggtcaatatc tctctcgata tttcgccaga gatcagcttt
     7801 gtcggtgagc agaacgattt tgtcgaggtg atgggcaacg tgctggataa tgcctgtaaa
     7861 tattgcctcg agtttgtcga aatttctgca aggcaaaccg acgagcatct ctatattgtg
     7921 gtcgaggatg atggccccgg tattccatta agcaagcgag aggtcatttt cgaccgtggt
     7981 caacgggttg atactttacg ccctgggcaa ggtgtagggc tggcggtagc ccgcgaaatc
     8041 accgagcaat atgagggtaa aatcgtcgcc ggagagagca tgctgggcgg tgcgcggatg
     8101 gaggtgattt ttggtcgcca gcattctgcg ccgaaagatg aataaatatg tccttttacc
     8161 actacatcaa gtcattgatt cgatcaaaca attttcattg aaaaagtgcc aaaaatcgca
     8221 aattgactac accattaact acaccgttcg gtgcactgta tgaaacaacg tggaacaaat
     8281 agacacaaga aatatacagg cgggtaatct ttccaggggg aagcgcacca attcatgagg
     8341 ggcgttaatg tcgatatggg gatccccata acggggctac cggatttttt ttccggttaa
     8401 cctttaatca ggctggtggg ttttacatac ccttgatcac cgtaatgatg atccagatgt
     8461 gcaccttttc caccaatggc ggaaaagctc caaccgtaac gatgatcgct atggttgcct
     8521 cgatggtctg cgtggtcatt atgaccgttc agccagtgac cttaatcaga atttttcgct
     8581 aaaaaatgac gttggccacg tcatccggaa agcacaaaat cacgtcgatt tttaatgaac
     8641 ggttaaaccg tctttttgtc acggtgtgtc actggttgtc actctccgtc acgattgagg
     8701 tggtggtcat tctgcccgac cgcgcatctt tgcgctatcg ggaatatcaa acagttacct
     8761 cgccgacttc ctcaaattga ggtttccgaa aatatcagag cgggttagcg attaagctga
     8821 cgagtaatta aactgcgaaa atttcgtagt ttctgaatat attccggcct gctggttaac
     8881 agtcctcaga tgtgagggat gtagccagcg caattttgcg ctttgcttta atctcagtga
     8941 gttaccgccg taaccgctcc ggcttcttcc agtggtacgt gattttctcc ttctcccgat
     9001 acagttcaac gcggcgattg taggccagca tttccagaac gcggatccgt atgtcgcgca
     9061 tatccgcatc attaagctgg ataccatcac ggcgcatcac ctcagcaaca acacgcgcat
     9121 aattttcggc tgtcacgctg tccggctgcg tggtctgttc gtcagcctgc tggctgattc
     9181 cagagacgcg gcggattaat cgcagtattt cggcttctgt catgctgctg acctcattac
     9241 accccgctaa attcttccag tttctggcgg tggctgtcgc ttatatcaaa agcaaaatcc
     9301 tcatgctctg cctggaatgt accaaacgcc atcagcgccg ctacgctcgg gtctatcttg
     9361 ttcggtgatt ttttcttgtt cggcttgata ttggcgttcg cgtcactctg catcacgacg
     9421 ttactcatcg accaggacaa caccggatcg ccacgatgca caatcaccct gcggttaaca
     9481 aaaacttcga acgatttcgc cgccggactg aatctgaggt aggtttgcgg gaacggctcc
     9541 acctcaaaac cagccccctg taattgcgtc ctgagatgcg tggcgttcca cgtatcaaag
     9601 cccaccagcc tgatattaaa tttctccgca tcctgcatga tgtcatctct gatccggtcg
     9661 taatcaatgc agtcacctgg cgttgtgcgt atccagcccg ctttagccca ctggcgatag
     9721 acagcgcggt ttttattggc ggggttctgt agctggaact ccggcagata atgacgggaa
     9781 atcagcataa tggttttacc gaccggaaag gcatagcata cgctggaaat atcgctggtt
     9841 gatgataagt ccagccccgc gtaacactcc tgaccgtgta aatcttcctc cgtgaacgtt
     9901 ccggcacact cagcccatgc accattaccc atccacgggg tagtcccctg acaccagata
     9961 ttaaatcgct ttgtcatcat ctccacccac tgcgacggaa taccccgcgc tttctggatg
    10021 gttgaggcca gtttttcacg atccacggaa acatcaatat tagggttcgc ctttatccac
    10081 atcgccggat catcaacctc gttttcgtcg tccagttcgt aaatcagtac aaaaattgaa
    10141 tcgttaacct cttcgccgtc caggatctgg cagcaataat catagtgctg tttacaggct
    10201 gaaacgacgt tactgcccga tgtggtaatg gcaaataaca gcccttcggg acgtgcgccc
    10261 atccccagct caagcgcgga ataaacgccg ttatcggggt gtaggtggta ttcgtccacg
    10321 atggaaagac tggggtttgt cccctcgatg gttgccgctt ttgctgccag cggctttaac
    10381 aggctgttgc ttttcgggtg tatcacctta tgcgcctgaa tattcacccg cctgcgtaac
    10441 ggtcgggata aaaggcacat ctgacgcgca ttatcaaaca cgatccgcgc ctggtcacgg
    10501 ctcaccgctg cggtgtagat atcctgctga ccgttctcca taatcagaaa ccagttagcc
    10561 agaatcgcgg cggtcgtgga tttcgcattt ttgcgcggca cttcgataaa ggcgctcgtg
    10621 tatttgcgcc gtccggtggc cttaacctta aagccgagga tgcacgcaaa ggcgaactgc
    10681 tgccacggct ccagctcaat gggtctgcca cgcatcggtc cttttacgtg cgggcacacc
    10741 ctggaaaagg caataaaccg ctccacaacc tcacgatcga acgtgtaaag ggggctttta
    10801 aggtccgaaa agtaccgttt aacagcctgt tttaaccgtt tacaggccgg aattttgccc
    10861 gtttttacgt cttctgcgta cttattccag gcggtcaagc tcgtcctctt cttctgtttc
    10921 cggtggattt ttacggcggc ttatcgggtc aaaaccgagc aaggaggcca tttttatcat
    10981 cactctttca gcgtcggatt ttgcgcttaa tgcggggttt ctgctctcgc cgccctgact
    11041 gttaacaatg ctgaacccgc gcgccgcaag gtctgcaacg gctttccggt aaatggagta
    11101 gttgacgcaa tacagttcca gattgctcca gtcggcgggg gtcaggtctc cccgttccgc
    11161 aagctgccgc gatttttccc gccactgctt cacggcgata tcatccaggt aggcggggtc
    11221 gggtttgtcc tgaaaacgcc ccccaccccg tcatacagcc tcatcagcga ttgcggaaac
    11281 attccataac ctcgcggtca cggtcggtta atcgcttcgc tgtgatgcgt tctgcgcgtc
    11341 ctgacgcttt atctttatgc cctgtttcct gtgtcttcca tgcgtcacgc tgccttataa
    11401 gtccacggat aaggcggttt tgttcccgct cattcatcag cgtcatacat ccagttattg
    11461 cggttagcag cccgttcttc ctcttcacga aatccacctg cggcacgctt gctttttgtt
    11521 gccggatcaa gccatttcgt tttctggtta tgacacgcct ggcacaatgg ttgatggttc
    11581 cattcgggcc agaagagaac atcatcaccg ccattaatcg ggataatgtg atccaccacc
    11641 acggcgggcg tatatatccc cttctcaagg catcgcacgc ataacgggtt tttactcaga
    11701 tacatggcgc ggtatttgtc ccactgtctg gaatatccac gcgcgcggcg gtgtcctcgt
    11761 ctggcatcct ctgcacgcca tgccgcccgc ctgtgctcct cacacttgcc ggatttaacg
    11821 cgcctgttac agcctggttc tgtgcatcgt cttaatggtt gccacggcat cagtaccccc
    11881 ccacatcacg gtaagccgtc cagagtgcgc ctatcgtcat gggtacgcgt gtttttgcgt
    11941 tatccgcgac aatctggcga ttttcataca ggtgagcgat aaacatcatg cagccaatct
    12001 ttatggctgg cgtgaacgcc agcccgtcat caaagcgcct gcctatgtgt atctggcacg
    12061 cctcaagcga tgcggcaatg tatccgctga ttaactggtc ttcatcgtcg ccatcgatgc
    12121 ggcaatggag tttcacttct tccagggtga taagttcttc tgtcattttt ccgcgccctc
    12181 acgacaaaga atttcaaggc gtgtcctggc ggcatccggc agcggctgcc cgatgatatt
    12241 cagtacacgc cccgccagcg gccccgtact gacctttatc cggctggtgg cgttgatgtc
    12301 cttccggtac cgtatccaga tccttacggt accaatcgcc agctctgcgc ccgatgaaat
    12361 ggcctccttg ctgctgacca tgttcacgct tgcccagagt gtgtgaccgt cctcccacgt
    12421 ttcgagtatt tcgccggtta tatccctggt ctgtttcagg gtctgaatcg ttatcctgtc
    12481 acgcagtcgc cctatgttca ttccggtttt tctccctcgc tgatttttac ttcctgtttc
    12541 catgcctggc tgaactcatc accaccatca cgcggtgaca gcccttcacg ttcgcgggct
    12601 tcgttcggac acataacgtc ggattttatg ccgcgctcat aggtggcaaa gcgttcgcca
    12661 ggtgtggccc gtaacaggtc cgcgctgtca aactccacct gataccggat accaggtaca
    12721 ggcgaggcca ccagcaaggc gttttttatc tgctgctcaa agttcgccag ccacgggcgc
    12781 atcgtcatgg tgagaaatgc gcggcttgcc tcgctgaagt tgctgtaggt gctgttgctg
    12841 tattcctgca aaaaaatcgg cgagacgttg aacattcggg cgatgtcttc aatggtgaag
    12901 cgacgggagg ccagccattc ggcatcctga ttactcatgc ccagctgctg atagctcatc
    12961 cccccttcaa ggatgggcgt ttttccggcg tttctggccc ctttgtagcg ttccagtgcg
    13021 gctaatgcct gtttgccctt cacgccgtcc agccattcgc ctgacgtgat aacccctgcc
    13081 gccatcatgc catcacgcat cacgctcgcg ccgtggcgtt gttgggccag cccaagcccc
    13141 agcgattcgc ggcagatggt tacaggtgag cgccccataa aaccgtcatc cgtggagtaa
    13201 cgaaggtgga gaacctccca cggtaaatag ttgcggatgt ttccggtgta ggcgtcagtg
    13261 atgcagtagc gccagttatg atcacctgtc tgctccacgt tcaccgactg cggcgggtaa
    13321 ggatgtaaag ccgccggaaa accatcacgc ccccactgaa taaccgcata agcattacca
    13381 cttaacaggc agtagcggat catcattcgc ttaaactggt agggtgtctg ccacgcgttc
    13441 gggcgctcgt tgaggatgtg atcgaccgga tgagaatcaa gccattcgcg ggcctccttc
    13501 cccttctcat tgcgtaccag gtacaggtaa cacggcatgg tagccaccgc gtcagagatg
    13561 accgtgacgg cgttcatgac ggcggggagt gattccgctg tcccagatga tacgtactca
    13621 cccgcccccg tgttcgatgt gccggccagc gccataaatt catcaagcgt catgctgcgc
    13681 tgctcttttt ttcgtctgaa aggccacata tcacaccccc gctaaatccg cccaccagcg
    13741 gcgattatcc gcacgcggca ttttttcggg gtgctgctca tacagggaac ggcgggccag
    13801 ctccacgccg gaatcggggt aagccggtac cgatgtaacg gtaatttcgt acagttccgc
    13861 caccagcacg gtgcgcacgc atggatctgt tgtggtatcc catacatcct tacgggagcg
    13921 aaagccaaag ctcatccccg agatgtcccc acgcttaacc agttcgataa cgtcgcgccc
    13981 tgtactggta tccggtgggg tcagttcaaa gcgtaaccct gtttcgtcct cttccagttt
    14041 cagcgtgccg gaacgggtgc gccccagtaa catgctgtgg tcatgctcat acaggccgcg
    14101 aacgtcatta cccgccgcaa gccattcagt aaacgccccc cgctggaatt tttcgtaaaa
    14161 ctcaccccat aacagttctg aaagcttatc ccagcgaaca acgtagcccg tcagcgtacc
    14221 ggctccgctg gtggtgattt ccgatgaccg gatttccata ctcttcataa ttttttcacc
    14281 cataaaacac taaaggggct ttttagcccc ttctgtatgc tgttaatcgt cgtcttccgg
    14341 cagtgccaga atcttgatcg cgttcgaatc caccacgcca ccgcctaaat atttttgcgt
    14401 gaaaattttg atgaatcctg gttctgtcag gttgtccggt ctggtgcgaa cacctgtttc
    14461 gtgatcaaca atgtagtaac cgcgtttgaa gtcacccagg gcaataacgt tatcaggcat
    14521 aaactccaga tattcgaccg gaaggcccag caacgtatca ggatcacccg cctgtaaacg
    14581 gtcgcgccag atataatcac cgttcgcgtt cttcaccttc tgaagttttg ccgccgtcgt
    14641 ggagttaacc actcagaccg cgtttttgcg gtatttttta cgtaatgcaa atttcaggtc
    14701 gatcagcggg tccgcagatg tccacgccag agattcggaa ggtttaatta cctgcaacgt
    14761 accaaaatca cgctctttgt cgttcttctc tgcacggggt acggataaaa aaccttttgc
    14821 ttttttgtca ccgtcgccca caaccagatc gctttcttcg gtttccgtga aggtgtcgcc
    14881 aatctcaccc gtcagccatg aaaggatgtc cacatcggaa aaatccacga tttcctgtgt
    14941 ggtgcgcggg tacgcataga ccggatacag cttaatgctc acctcgttaa tctgcggggt
    15001 gctggtctgt tcgcgtgcct taccctcttc accgtggtta acggtcgcac cgccagcgga
    15061 aacaagctgc ttaaactcgt tgctgctgat tttcttcacg gtacagatgc gacgcatggt
    15121 ggattcatcc gccagcattc gcatgatttc ggtgttcagt tcagggataa cggtataacc
    15181 accatcagcg ggaacgcctg tacttaatgc gcgggtttca ccggtcagaa tgtagttacg
    15241 tagttctgcg gggtcagtgg tctggctgct tttacctggc ttgctgcgct cttcgtctgc
    15301 aatggcttca aggcgggaaa tgtctttatc gagggattca gctttagcgc gtaattcgtc
    15361 aaattttgcg ccctcagcat cgttaagact gcggttttct ttttcagcgt tctccagcat
    15421 gtcgcgcatc tgatttttaa tggcggtttt ctgctggcgt aattcgatta ttctctgcat
    15481 aaaaaaagtc ctggggttaa gtaaggaact ccaggacgcg gcaaacactc aaccgttttt
    15541 cataaggaaa tcagtaatcg caccgatcgt tttcccgcct ggtaatgaat attggcgagc
    15601 acattaacag gcgggaaagt ggccccagcg tcctggcacc acgggcgaga ataatcatga
    15661 ttcagttcgg gtaaaatatg ccgatccgct cagtgaacaa cgtggaacaa ccacgaacaa
    15721 ataatttaca aaaaataaca aaaagccgga ttgctccggc tgcgtggtgt ggttctgata
    15781 ttcctactct gctaatttgc tcagccccat gtctgcgtaa gtgcgattta ctgcatttct
    15841 caggtctgcg tagttctccg ttggcggctc cggtgtgccc ttgctgctct gttttaccag
    15901 cgggtcgccc accagctgcc cgtaagctgc aatttgtgct gtcatattgc cacctcagtg
    15961 aagcgatccg gcattgttct ttttcatctc ttccatcatt ttttcagtga gcattacgca
    16021 ctgaaacccc atgtttttga tgtctacggg tttcatgcct ttgtcagtgg atatcacagg
    16081 cagaatatcc tgatacacat cgcggtcagg gtaactaagc acacaaagct cccatgatgc
    16141 cacgggtgag gatccttcgt ctgtttcaac ccaccagcct ggtgtagctg cgcttatttg
    16201 cagaatcgtg atccgtccgt tgtgcttgta aaatttttca ccaaattttg gttcgctcat
    16261 gattccacct ctccggtttt aacgttgatt gttgttacct gttccgcttc ggcaatctcc
    16321 cgctctgtca gcgtggcaaa atttgccgcc gtcgtggtca tgaatgcgct tatcagttcg
    16381 ggatgtgctt tcgcgtatcc ttcccccgcg tggcggtcta tcgttctgat tgccaccttt
    16441 aaagcgtgct ctgtcatgtc taacgcgcga tatttcggtt ctgttctgtc tctgcgtttt
    16501 ttgaatgatt tattaaattt ccctgaagtg tgcatattta tttttacccc ctcgtttaaa
    16561 aagttttgag ttgtgcctcc ccttgtctac cttatctacc ttagtggccc tcatgccagt
    16621 aatggcgctg ctttcagcgg ggtagagtgc ttttatccac tatctacccc gtgtctacct
    16681 ccctgtctga ttcaggtaaa atcaggtaga gagggtagat agtgggtaga cagtaaaaaa
    16741 aggctatcta cctaacttaa tgcactgaat taaatgtatt tttctttact caggtagaca
    16801 gggtagacag caattacaaa aaattataaa aacgcgtcgc actcgtctgt tgttattgcg
    16861 ttagtctgcg ttactccctt aactttccgc gtaatatatt catgtccgta aactttcgcg
    16921 gctggcttca tagccttgcc aaagtcattt acgtttagcg gtttgctcct gcctgcgtac
    16981 gccataaacg ccagatagac gcggtaaagg ctgtttctgg tcgtgtactt cactgaatcg
    17041 ccaccgccgc ccatcatcag gccgcgtgct tcctccagaa aattcaggaa ctggcaaaac
    17101 tcaataaccg gatccgtctg ttgctttatt gccagagctt catcaccgtc acgctgttcc
    17161 agtagtaaag cccgtgcttt ctcagagtcg gtaaagttcg ccagcaatcg gcggataatg
    17221 acagggattt cagccgcaat cttttccggt agctccctgt ctttttcggc ctcactaacg
    17281 atattgtcga aacggaaaat cacgcgacga cgtgccacac ctccggcccg ttcggtgaat
    17341 atcatcgggt tattgttggt cgccagcacc accgccctga ttacagccgt gaaacgcttt
    17401 tcgtatttcg ggttaatttc cacggggtca ccgcccgtga ttttcttgat gcccgttcct
    17461 tcgcctgtat atttcggctg gtcagccagg acgataagac gactcccgac aacctgcgca
    17521 cgtccaccag catcatcaag cgatgtcatt tcagcgctta ccgtgttctg tttccctgcc
    17581 agaaggctgg ctatgtgtgt gaatgtactt ttaccgctcc cgccgtctcc ggtggcctca
    17641 ataaacatct gccagtcgta ccggttcgcc ataatcatgt acagcgcggc acatatacgc
    17701 atcatcttgc gctggtcttt tccggctgcg tgctcaagcc atttatgaaa gtttagcgcg
    17761 ttatcgcgga tgttctcccc tggtgctggt ggcgtgtact caatgccgtt gtgcgtggtg
    17821 atccagttct ccggcgtgtg cggggaaaat tcccccgttt tcaggtcaag cgcaccattg
    17881 gcgaacggca gcaaatcgcc ggacggctcg cccattggtt cggcaataac ttttaacgct
    17941 tccacggcgt tattgattac gcgcttgctg aaagtggccc tgtgctctga atagatcacc
    18001 accatttcgc ggctaagttc cattgtgctg accggacacc ataccccgcc gcgccatacg
    18061 tgaacgattt cactttcctg atgcacacaa acgccatcaa agcgctcggc aagcagctgc
    18121 gcgcgctcac tgtctgccat ttgtgccagt tgcgtatttt tctgctccgg catggtaagc
    18181 cctgcggcaa tattttcacg ctcagcattc agatagcgcc gccagttttc acacttctgg
    18241 acgtgcattc cttcggggta aaaatttgcg tcctgaatat ctgccgccgc cagcttctga
    18301 ccaatctttt tggtctccac caaatccagt tctccggcct ggtacagcct tacgcgtttt
    18361 tttccatccg gaacaatttt cagtgcatca agttcggcaa gctgatttgg cccaagccac
    18421 acaggcggca cattatcgcc ggatgcgggg ccatcctgct cctgccattg tttcgcgtgc
    18481 gaccaggcat cactacccgc aaagataatt acctctgtgt ctttgtgttt tattccgcgt
    18541 ggctgttttt ttacgttcgg tgccagtttc attttttacc cctgaatacg ttaagcatct
    18601 tttttatttc ctgaatattg gcgcgtgctt tctccctgct ggttggttta ctgcggggcg
    18661 ctgcctgtac cagcgtgaaa tcacgccgga attgataaac aggcatcacg caatcatatt
    18721 cgtaaccttc acggcggtag gtgatgcgcc gttctgccac gcctttaatc gttaccatgc
    18781 cgccgtactg gtcgcggtaa atatcaccgc gcgtaaattt agggtgagtg ttgccactgg
    18841 cagttaagcc agaatattta agtttcatta tttttattct ccggtgtgtt gttctttata
    18901 tctgtcgtgc aatagatcta tttcttgcag ttccattatt acaggctcaa gaagcgttat
    18961 taatgccgtg gcaattcttg atttttgttt gtcgcgttca ttgtcgccaa gtgtttcaag
    19021 ccatatgcgc aatatttcca gcatgttttc actgtgagaa agtgcaagaa atgcgcggtc
    19081 tattgtttcg tggtaaatat cacgcatatt aatccccgtc cgtcgttttt cttaaaataa
    19141 cctctgtcac gaaatcagca taatcggcgg cgatattcag tacatcaagc ccgttgattt
    19201 atattctctc gtggaaagta agaaaaaagc cgctctaata agttctggca ttgacgaaag
    19261 cgcatcagcc gcatcatcag gaacgccgga aaattcctgt ttcagggaat taaaacgatc
    19321 atcacgcata acccccccca ttttcacaat cggcaacaag aatattttta gcgtcattca
    19381 gtgaccgcgt tgcgatgtat cggatacatt ccagggcgaa ctgtgtccgg tctttctgtt
    19441 cttctccctg cgcaagctct gccgtgcatt caatatcaat aagcgcgtgc atcagcgtag
    19501 tgagtgcggc ggctgctgcg ttcggtgtgg ttttattgtg cataggtttc cccctgacga
    19561 atacgggcgg cgaatacagc aacacaaccg gacgggcaac ggctacgcgc ttcgcgttcc
    19621 gtccaagcgg ttacgtggat gatttgggat tctccggcac tcagtgccag aaaacgccac
    19681 acaaaggccg tttgtgtgtg tacaaggtgt ggtatatgat ttacggcaac cataacggct
    19741 cctagtttac gttgttggtt agaagccccg ttactgctcc tacagtgcgg ggctttgctt
    19801 tttgtgttga ttgtgtgtaa tgtgtcacta cacataaata cattacctga ggtgtaatta
    19861 acgtgtcaac acacaaaaac gaaaggcgtg gtaatcctcc gttccagttc cgtttagatc
    19921 ctgacttacg ggaaaagatg gaaaaggcac aacaacagga cggcgatgat tctcttgctg
    19981 cctggataaa gcgaataatc cgcaaagaat tacagcaacg cggcatagaa cccaaaggat
    20041 aaaaactatc agcgccgtgg tgtgaggtac tacggcgcat tgctttacag gcgcacacaa
    20101 tgaccaacga agaatcaacc gaaaaaacat cacctgcacg aaaaagacga cgcagaaaga
    20161 aagagcatga atcagaacga ttcgcgccgt gttcttttgc ccttgagaag taccttaaag
    20221 aatactcatc aaaggagaga acttcgcagt tatggcaacg cactgaacca gactgattgc
    20281 attgcccacc agcctgatag cggctatcat ccccgtgctt atgtttggga tcacacacac
    20341 aaacggcgca gcgggttatc tgttcagaaa ggcggctcca tttcggggcc gcttttttta
    20401 tgcctgaaaa accccaattt tgtggttttc cagtttcacc agggcgaacg aatccccgcc
    20461 cacgttctgg cgtatattca atcttcatgg ttatagctct gtgttcagat gattgatgtg
    20521 tggcggctgc gtgccgccag cgtgattaat gaactgcctt gcagctatcc ttccaggcca
    20581 gaacctcgga taaagaccag ccaacggaac gaccgccaag tttacgacgt gatgggaatt
    20641 gtccggcctt ttccaggcgg tagcggcatg agcggctaag gcctgttagc ttttcgcatt
    20701 ctttttcacg tataaaccga tcagtgctta acactattgc cccctttcgt ttcttaaaga
    20761 gttatttcgt gttctattgc gttgggatgt gtctgattgt gtcaggatga ttcagagttg
    20821 gcaaatgttg aggccgtatg gtttacagaa agaggaataa tcaggataaa atcctttaaa
    20881 ttcatgttaa tacaaaggca taaaatattg tttcatgcct tttttctcgc tatttaaaga
    20941 gtgattcgct agtgtataaa aaatcagtaa cacattaaaa atcagctact tataaatctg
    21001 tacacttttt cacctcttgt tcgtagttgt tccgcattgt tgctcattgt tgcacgttgt
    21061 atctgttcgc atatccagta tgcgcatact gaaaaaacac gaaaaaaatt attttcttct
    21121 ggctactggt agcgtggtta cgttttcatg tgttcccgcc agtatcccta accgctccgt
    21181 ccacatatcc agcgcattgc gtttagcatc cagataacgg gaatgattat aaactcgctg
    21241 cattcctggc atctggtggc ctgtaagctg ctccacgacg tgaggatcca cgcctaaatc
    21301 gttcagcatc gttgtaaagg tgcgccggat gtcatgtagt gaccagtgag ggtgatttag
    21361 cctcctgtgc gctaatctgc cgtactgcga cacgcttgtt tcctgtttca cttcccccag
    21421 caataagccc gtgtgcctgt tctgctccac cagctgcgtg acgaacggca gtattgcttc
    21481 cggtatgggc cggaatattg ccaccttcgt tttgctgtgt tctttcggca cggtccagag
    21541 catttcggta aaatcccact cgctgatctc cgataacctc agttctaccg tccggcatcc
    21601 gaacacaatc aggaggcgga ttaacgcgat gtagtagggg gagaatattt ttttgtccag
    21661 tgcctgcaat aattcgccca gttctttggt gcttaagaca cgctcgctta tatccggttt
    21721 tttcccaacg tccgccacac tcatatcatc aagaacgttg ctgattgcat agcgccgcct
    21781 ccggcagaac ttaagcgcct gtttgcacgt ctgtagcaag aatccggcag taacaggcgt
    21841 tcgctttgcc acctggtcaa aacaggccag ccagtgccgt agctcgcatt tatccagcgg
    21901 catagcacca atgtgctgta ttacgtgatt attaaggcgc tttttcaggg cggcataatc
    21961 cacgcggttt tcctttgcgt acgactcaag ccagtaggtg agcgcatcgc caaccgttac
    22021 cggctttaac gcttcctgta cggtgtaatt aagctcatga cgtggatttt tcccctctgc
    22081 cagccatgcg cgacactggg cggctttttc cctggctgat ttcaggctca gatcaggata
    22141 atttcccagc ttaatgcgtt ccggtggtgc cccccttccc gttccggccc tgtaagtgaa
    22201 ataccaggtt aaaaggccac tggttgaatg cctgacgctc aggtttccac cgtcattaag
    22261 aaaggctgtt ttttgggcgg gtgtgccgtt gattttcctc agctgtgtat cgctcagttt
    22321 gttaagtgct ctgctcataa ttttgattcc ggtcatcaca attaactaca ccattaacta
    22381 caccgatcgt tgcacaaagg gctacaacgt gaaacaagct ggaacaagga aaatcacaaa
    22441 tctgtttata atcaaaaaca taatgaacaa tctgaaacat tatgaaacgg caaaaaacac
    22501 taattgataa aatatgtcca tacttcacgc attacgttaa gcatccgtta taatcggttg
    22561 cagataccag cctgtggatg cttaacatgg aataccaact cactcttaac tggcccgatt
    22621 ttcttgaacg tcactggcag aaacgcccgg tggtgttaaa acgcggcttt aataatttta
    22681 ttgacccgat ctctccagac gagttggcgg gtctggcgat ggaaagcgaa gttgacagtc
    22741 gactggtcag tcaccaggat ggcaaatggc aggtcagcca cggcccgttc gaaagctacg
    22801 atcatctcgg tgaaaccaac tggtcattac tggtacaggc agtaaaccac tggcatgagc
    22861 cgaccgccgc gctgatgcga ccgttccgtg aactaccgga ctggcgtatt gatgatctga
    22921 tgatttcttt ttctgtacct ggcggcggcg tcggcccgca tctcgatcag tacgacgtgt
    22981 ttatcattca gggtaccgga cgtcgtcgct ggcgagtggg cgaaaagctg caaatgaaac
    23041 agcactgccc acatccggat ctgttacagg tcgatccgtt cgaagccatc atcgatgaag
    23101 agctggagcc tggtgatatt ctttatattc cgccaggatt cccgcatgaa ggctacgcgc
    23161 tggaaaatgc gatgaactat tccgtgggtt ttcgcgcgcc aaatacgcgg gaattaatta
    23221 gcggatttgc cgattatgtg ctgcaacgtg aactgggcgg caactactac agcgatccgg
    23281 atgttccacc tcgtgctcat cctgcggatg ttctgccgca agagatggat aaactgcgtg
    23341 agatgatgct cgaattgatc aaccagccgg aacactttaa gcaatggttt ggcgagttta
    23401 tatcccagtc acgtcatgaa ctggatatcg cgccgccgga accgccttat cagccagatg
    23461 aaatctacga tgcgctgaaa caaggtgatg tgctggtgcg cctgggtggt ctgcgcgtat
    23521 tgcgcattgg cgacgacgtg tatgccaatg gtgagaagat cgattccccg caccgtccgg
    23581 cactggatgc actcgccagc aacattgcgc tgactgcgga gaattttggc gatgcgctgg
    23641 aagatccgtc attcctcgcg atgctcgcgg cgctggtcaa tagcgggtat tggttcttcg
    23701 aagggtaagt ttggatttaa gccggatgca gcatcacatg ccgcatccct tttcgcttac
    23761 ttccgttgcg ccgttaactc ggcaatacgg acgatcacct gcaccgcttt ttccatacct
    23821 tccagaatca caaactcatg cttaccatga tagttgtaac cgccagtgaa caggttcggg
    23881 caaggtaatc ccataaacga caactgcgcg ccgtcggtac cgccgcggat cggtttcagt
    23941 tccggttcaa tatcgcagtc acgcatcgcc tgctgggcga tatcgagaat atgcggatgc
    24001 tcaaccactt tctcgcgcat attgtagtaa ctgtcttcaa tcaccaattc aatgtagcaa
    24061 tcaggatgta accctttgcc cacttttttg gcgatctcca tcattttacg tttacgcgct
    24121 tcaaactgtt tacggtcgaa atcacggatg atgtagtgca tatcggcccg ttcaacggtg
    24181 cctttcatgc tcgccaggtg atagaaacct tcatagcctt ctgtcatttc cgggctttca
    24241 tccgccggaa cttccgcatg aatacgtgcc gccagcgaca gcgcatttac catcactcct
    24301 ttcgccgtgc ccggatgaac attgttaccg acaattttta tattgaccga tgcggcgttg
    24361 aagttttcaa actccagttc gcctacgcca ccaccgtcaa cagtgtaagc ccagcgggca
    24421 tcgaaggcat caacatcaaa atgtttcgcc cctttgccca cttcttcatc cggggtaaag
    24481 gcgacgcgaa tatcaccatg cggaatgttt ttctgttgca ataccgccag cgcggtcatg
    24541 atttctgcaa tacctgcttt gtcatcagca cctaacaagg ttttaccatc ggtggtaatc
    24601 aacgtctgac ccagtagctg atgcagcacc gggaacataa ccggtgataa aacttcatcg
    24661 ccgataccca gcgcaatatc gccaccgcga tagttttcaa caatttgcgg attaacattt
    24721 ttgccgctgc aatccggtga ggtatccaca tgagaaataa agccaatcgc cgggatatcg
    24781 ccagggacgt tagccggtaa cgtcgccatc aaagtgccct tctcacttaa ggtcacattg
    24841 ataagcccca tttcttcgag ctgctctttc agcagatgca ataacttcca ttggccttcc
    24901 gtgctgggaa cctgtctcac ccctgctttt gattgggtat ccagagacac gtagttcaaa
    24961 aatcgctcaa gtagtttatc catgtagtca ccctcacttt ttgtgacaac attattaaga
    25021 agcaagaaaa gacaaatatt gcgtcaggtc acttttaccc ctgcaagcgg gaatatttat
    25081 cagcattacc ttaatgaata taaagctaag ccagtaattc acaacaaaga ttggcgattc
    25141 aagcggtttg ccgtagaatt accgccctca ttaggagtca ccaaggtggt taaccacaaa
    25201 ccccgcatcg gtaagccatc cgttgcgttt acatgggaca gagtaaaaaa ttgaataaac
    25261 aaccgagttc gctttcaccg ctggtgcaat tggcgggaat tcgcaaatgc tttgatggta
    25321 aagaggtcat tccccagctg gatctgacta tcaacaatgg cgagttcctc acgctgcttg
    25381 gcccttctgg ctgcggtaaa acaaccgttc ttcgcctgat tgcaggtctg gaaactgttg
    25441 attccggacg catcatgctg gataacgagg acatcaccca cgttccggcg gaaaaccgct
    25501 atgtgaacac tgttttccaa agctatgcac ttttccccca catgaccgtg ttcgaaaatg
    25561 tggcctttgg gttgcgcatg caaaaaaccc ccgctgctga aattacgccc cgcgtgatgg
    25621 aagccctgcg gatggtgcag ctggaaacct tcgctcaacg taaaccgcat cagctctctg
    25681 gtggtcaaca gcaacgcgtc gccattgctc gagcggtggt taacaagcct cgtctgttgt
    25741 tgctggatga gtcgctctca gcgctggatt acaaactgcg taagcaaatg cagaacgagc
    25801 tgaaagcgtt acagcgtaag cttggcatta cattcgtttt cgtgactcac gaccaggaag
    25861 aagcactcac catgtcggac aggattgtgg tgatgcgcga tggtcgcatt gagcaagacg
    25921 gcacgccgcg tgaaatctac gaagagccga aaaacctgtt tgttgccggg ttcattggcg
    25981 aaattaatat gtttaacgcc acggtgatcg agcgactcga cgagcagcgc gtacgcgcca
    26041 acgttgaagg ccgcgaatgt aatatctacg ttaacttcgc cgttgaaccg gggcaaaaac
    26101 tgcatgttct gctgcgcccg gaagacttac gtgttgaaga gattaacgac gacaaccacg
    26161 ctgaagggct gattggttac gttcgtgagc gtaactacaa aggcatgacg ctggagtcgg
    26221 ttgttgaact ggaaaatggc aagatggtga tggtcagcga attcttcaat gaagacgatc
    26281 ctgactttga ccactctctc gaccaaaaaa tggccattaa ttgggtagaa agctgggagg
    26341 tcgtactggc tgatgaagaa cacaagtaag ttccagaatg tagtgattgt cactattgtc
    26401 ggttggcttg tgttgtttgt ctttctgccc aacctgatga tcattggcac cagctttttg
    26461 acccgcgacg acgccagttt cgtcaaaatg gtctttacac tggataacta cacgcgtctg
    26521 ctcgatccgc tctattttga agtgctattg cactcgctga atatggcgct gatcgccacc
    26581 ctcgcctgcc tggtgctggg ctatccattt gcctggtttc tggcgaagct gccacacaag
    26641 gtgcgtccgc tgctgctgtt tctgctgatt gttccgttct ggaccaactc attgattcgt
    26701 atctacgggc tgaaaatttt cctcagcacc aaaggctatc tcaacgagtt tttgctctgg
    26761 ctgggcgtta tcgacacacc aatccgtatc atgttcacgc ccagtgcggt gattatcggt
    26821 ctggtttaca ttctgctgcc gtttatggtg atgccgctgt actccagtat cgaaaaactg
    26881 gataagccgt tgcttgaggc ggcgcgcgat ctcggtgcca gcaagttaca gacttttatc
    26941 cgcatcatta ttccactgac gatgccgggg attattgccg gatgtctgct ggtgatgctg
    27001 ccagcgatgg gcctgttcta tgtgtccgac ctgatgggcg gtgcgaaaaa cctgctgatc
    27061 ggtaacgtca tcaaggtaca gttcctcaat attcgtgact ggccgtttgg tgcagccacc
    27121 agcattacgc tgactatcgt aatgggcctg atgttgctgg tttactggcg cgcttctcgt
    27181 ttgctgaata agaaggtgga actcgaatga tcggtcgact gcttcgcggc ggttttatga
    27241 ccgctatcta cgcgtacctg tatatcccaa tcattatttt gattgtgaac tcctttaaca
    27301 gctcgcgctt tggcatcaac tggcagggtt ttaccaccaa atggtatagc ctgctgatga
    27361 acaacgacag cctgttacag gcagcgcagc attcactaac aatggcggtg ttttcggcga
    27421 cgtttgctac gcttatcggt tcactgacgg cagttgcgct gtaccgttat cgctttcgcg
    27481 gtaagccgtt cgttagcgga atgctgtttg tggtaatgat gtcaccagat atcgtgatgg
    27541 caatttcgct gctggtgctg tttatgctgc tgggtattca gcttggcttc tggtcgctgc
    27601 tgttctcgca tatcaccttc tgcctgccat ttgtggtggt gacggtgtat tcgcgcctga
    27661 aaggttttga cgtgcggatg ctggaagcgg cgaaagatct cggtgccagc gaatttacca
    27721 ttctgcgaaa aatcattctg ccactggcaa tgccagcggt ggcggcgggc tgggtgttaa
    27781 gctttaccct gtcgatggac gatgtggtag tttcttcatt cgtcaccgga ccaagttatg
    27841 aaattctgcc gttaaaaatt tattcgatgg tcaaagtcgg cgtatcgccg gaagttaacg
    27901 cgctggcaac catattactg gtgctgtcgc tggtgatggt tattgccagc cagcttattg
    27961 ctcgtgataa aacgaaaggt aacacagggg acgttaaatg aaaaaatggt cacgccacct
    28021 gctcgcggcg ggtgctctgg cactgggcat gagcgccgct cacgccgatg acaacaacac
    28081 gctgtatttc tacaactgga ccgagtacgt gccgccagga ctgcttgaac agttcaccaa
    28141 agaaaccggt attaaggtta tctattcgac ttacgagtcg aacgaaacca tgtacgccaa
    28201 gctgaaaacg tacaaagacg gtgcctatga cctggtggtt ccttcaacct attacgtcga
    28261 taaaatgcgt aaagagggga tgatccagaa gatcgacaag tcgaagttaa cgaacttcag
    28321 caatctcgat ccagacatgc tcaacaagcc ttttgacccg aataacgact actccattcc
    28381 gtatatctgg ggtgcgacgg cgattggtgt taacggtgat gcggtggatc cgaaatctgt
    28441 caccagctgg gccgatctgt ggaagccaga gtacaaaggc agcctgctgt tgaccgacga
    28501 tgcccgtgaa gtgttccaga tggcgctgcg taagctgggc tactccggta acaccaccga
    28561 tccgaaagag attgaagctg catataacga gctgaaaaaa ctgatgccaa acgtcgcagc
    28621 gtttaactcc gataacccgg ctaacccgta catggaaggc gaagttaacc tcggcatgat
    28681 ctggaacggt tctgctttcg ttgcacgcca ggcgggtacg cctattgacg tggtgtggcc
    28741 gaaagaaggc ggcatattct ggatggacag cctggcgatc ccggcaaatg ccaaaaacaa
    28801 agaaggcgcg ctgaaattga tcaacttcct gctgcgcccg gatgtggcaa aacaggttgc
    28861 tgaaactatc ggttatccaa cgccaaacct tgcggcgcgt aagctgttaa gtccagaagt
    28921 ggcaaacgat aagacgctct acccggatgc tgaaaccatt aaaaatggcg aatggcagaa
    28981 tgacgttggc gcagccagca gcatttatga agagtattat cagaagctga aagcaggacg
    29041 ttaattcagc aagcgggcgg tggtattacc gcccgttttt tagttagcaa ggatgtggct
    29101 aatgagtcag gacagtaaag ttttatcccg ctctttcgtc ggtattgtgg tccgcttgta
    29161 ggcctgataa gcgtagcgca tcaggcaatg cttcctgctt tataatccct ccagcaactt
    29221 ttcaacaaat tctggcacca cctggcttgc cgggccgtaa tatttctcgg caaattcatt
    29281 accaacctga ctcggttcaa gattcagttc aacggtgtga gcaccatgta gtttggcttc
    29341 gtgtacaaac ccagccgccg gatagacatg cccggaagta ccaatggcaa taaagacatc
    29401 cgccatcgac aacgccatat aaatttcatc catgccgagt ggcatttcgc caaaccaaac
    29461 gacgtgcggg cgcaagggtg ccgggaactg gcaacaatgg catttatctt ctggcgtaac
    29521 gtctccggtc cagtcgagaa cctgaccact ttgcgaacaa cgaactttca gcaattcccc
    29581 atgcatatga atgacattgg tattacctgc gcgttcatgc aggttgtcta tattctgcgt
    29641 taccagcaaa aagcgatcgc cgagagcgtc ttgcagttta gccagcgcaa gatgcgcggc
    29701 gttaggctga atttctggct gctgcagctg tcgacgacgg gcgttataaa acgcttgcac
    29761 cagttcagga tcgcgatcga aaccttccgg cgttgccaca tcttcaaccc gatgttcttc
    29821 ccacaggcca tctgcggcgc gaaaggtacg aatacctgat tccgcagaaa ttcctgcccc
    29881 tgtcagtacg agtactcttg gtttttccat tgcttccggc accactttat ctctgaaaaa
    29941 aatacgctga cgcaaacgct cgcgcaggcg acgtttattt ttacgaaaac gacttaaccg
    30001 atgaccccga cgcgacagca tagcaacctc tgttgtttaa tcggttagat gtaggaaggc
    30061 cgcaccacgc attcctcccg catcaccgtg gcgcgcgcgt tcaatgcgtg gaacacgagc
    30121 tacaggtaag agatgacaag gcaacctgtc cgccagttgc gttgtgattg ccgggaaatt
    30181 cgataagcca ccaccaatga cgaccaggtc agggtcaaca atggtcagga tatttcccag
    30241 agaaaccgct aataaatcca gataacgctc aacgtgcgcc cttgcctgct catcgccttg
    30301 atcataaagc gcaataattt cgggagcctg caacggttga tgataatagt gttgatacag
    30361 ccacgcaaaa ccgcgaccag acagataatt ttcaatgcag ccatgctgac cacagccgca
    30421 gcggcgtaac gggaaatcca accccatcat ggttaacgca tcaaccggca gacgcatatg
    30481 gccaaactcg ccggtaatgt agcttttacc agtaatcggt ttgccgttga aaaccagccc
    30541 gccgccaacg ccggtgccga gaattaaccc catcaccagt ggatattgcg taaattcatc
    30601 atcccaggct tctgaaaggg caaaacagtt ggcatcgtta tcaaggcgta catcgcgatc
    30661 aagacgtgcg ctcaggtcgg cacgcagcgg tttgccgctg gcagcgggga cattagcggc
    30721 atacagctta ccatcttctg tttccggcat tcccggaata ccgatgccga cagaaccttt
    30781 acagccaaaa cgttggtcag cttcagctac cagctcacac actgcatcta aaaatgcgtc
    30841 atagctgtca cgcggtgtcg gcacccgctt ttcccactgc aactgccgac cgctatcaaa
    30901 cacgccaagc gcaatttttg ttccaccaat atcaaacccg taatacattg ccgctccttg
    30961 ttttaatgta ctgcctttac tggccgctaa ggactcgcgc agggtcaata ttactggcgc
    31021 gccgcgccgg ataccaactt gccaaaagac tcagcaacaa tgctgtgacc agtacgtaga
    31081 agacgtccag ccagtgcaat tccgatggca ggaagtcaat aaaataaata tcgctggaga
    31141 ggaactgatg accgatcagc ttttcaatcc actcaataat cggggtaagt tgcagtgaaa
    31201 caactacgcc gataaccacc ccacacaggc tgccgaacag ccctgccagc aatccatacc
    31261 agacaaagat ggcgcgaatt aaaccatctt tcgcccccag cgttcttaat actgcgatat
    31321 cgccactctt gtctttcacc gccatcacta aggtggagac gatgttgaaa caggccacgc
    31381 caatcaccag taccatcgcc agatacataa tggcgcggat catctggata tcgcgataca
    31441 tatagccgta agtaccaatc caacttttaa tataaacata gctgttggtc acttcacccg
    31501 catcgcgtac cagcttattg gcgttgaaaa catccgtcat tttaagggca atacctgaca
    31561 cgctggaacc catatcaaga tattgttggg catccgccag cgggatcatg gcaaaactgt
    31621 gatcgagttg accactcaac tgcaaaatac cggcaatgtg caaacgcaca cgttttggct
    31681 gcatcagttt atgctcggga ttcgagttgg ggatcatgat cgacacccaa tcgccctgct
    31741 tcactttcag cgcatccgcc acgcctttgc cgatgataat ttgctgttcg cccgctttaa
    31801 aattgcgcca cgcatccccc tgaacaaacg agggtaatgc gctcagacgc tgttcctgtt
    31861 gtgggttaac gcccttcacc tggattgcgc gcagattcgc tccactttcc accagaccgg
    31921 tgaaattgat atacggcgca gcggcggcaa tacccggcac tttctgcacg ttatccagtg
    31981 cttcctgcca gttagtccat ggctgattta ccgcttcgat ttcgccatgc ggcaccaccg
    32041 ccagaatgcg gttattcagt tcgcgttcaa agccgttcat cgcgcttaag ccgacgatca
    32101 ataccgccac gccgagggca atgccaatgg tagaaatgac agagattagc gacaccatgc
    32161 cgccgcgtcg ccgtccgcga ctaaaacgca ggccaatcaa taacgataaa ggcatcgcca
    32221 ttactccgcc cccatcaggc tcagttccgc cgtcagacgt ccatcacgca tttccagttg
    32281 gcggctcata cgtttcgcca gttgcaggtc gtgagtgacc accaggaaag cggtgccctg
    32341 caagcgattc aactccccaa gcaactggaa gatgctgtcg gcgttacgcg catcgaggtt
    32401 accggtaggt tcatccgcca gtaccagacg cggattattg accagcgcac gggcaatcgc
    32461 tacacgctgg cgttcgccgc cagaaagttc agatgggcgg tgattcgcac gatgctccag
    32521 ccccaccgct tttaacatct ccagtgcaca gctgttgatt tcagcgggct ttttcttgcc
    32581 aatcagcagt ggcatagcca cgttttccag ggcagtaaaa tccggcagca ggtggtgaaa
    32641 ctgataaata aagcccagct tctggttgcg cagttctgct ttcgccgctg aagacagttt
    32701 gctcatcggc tgaccgttaa agatcacatc gccggaggtt ggcgtatcca gcccgcctaa
    32761 caggtgcagc aaggtacttt taccggaacc agagctaccg acaattgcca tcatttcacc
    32821 ttcgcccacg ctgaaactga cattgagcag cacatcggtt tgcacactgc cttcctgata
    32881 gcgtttgcac aggttgtcgc attgcaacag gatcttattc ataacgtaaa gcctcagcgg
    32941 gttgtgtggc ggcagcgcgc catgaagggt aaagcgtaga cagcagcgcg atagccatcg
    33001 ccaccagcgc aataacaatg acctgtaaag gttcgatagc caccggcagc gccgcgccat
    33061 caagcaggac gccgattatc ggcatcagat tatttaactg gctggcaagc agtgcgccaa
    33121 gcgccgctcc gaggattgca ccgataatcc cggcgctagc cccttgcacc ataaagacca
    33181 tcatgatttg tcgcggagtt aagccttgcg tttgcaggat cgcgacttcg ccctgcttct
    33241 ccatcaccat cagccccagc gaggtaataa tgttaaacgc cgcaacagcg acaatcaggc
    33301 tcagcagtaa gcccatcata tttttttcca tgcgtacggc ctggaacagc tcgcctttac
    33361 ggtcacgcca gtcctgccat ttgctgcctt caggcagttt ttgctgactt aaagagtcaa
    33421 ctctcagcgg ctcatccagc cacaaacgcc agccggtaat attggctgcc ggataacgca
    33481 tcagacgcga ggcatcctca atattcacca gcatttcata gccatcgact tcactgttgg
    33541 cggcgaaagt gccaatcaca ttgaacaggc gctgacttgg aatacgcccc atcggcgtga
    33601 actggctggc agatggcacc atcacgcgga tttgatcacc gcgattgacg cccaactgtg
    33661 aggcaagttg ttcgccgagg atgacattat atttccccgg ctcgaggtca gtttgtttca
    33721 cattgaccag atacggcgtt agtggatctt tttgcgccgg gtcgataccg agcatcaccc
    33781 cgaccgccac gctgcgcgcg ctttgcagta ccacatcacc ggtagtaata ggtgcgacgc
    33841 gattaacgcc gtccagtttg actgccgttt ccgggagttg ctgcgggtta agagagccat
    33901 gctcagaaga gagaattgcc tgtggcatca ggccaaggat gttgttttgc agctcgcgct
    33961 caaagccgtt catcactgac aatactgtga ccagcgccat caccccgagg gtaatgccga
    34021 tggtagaaag ccaggagacg aaacgaccga agcgatccac tgcacgccca cgcatgtaac
    34081 gcaggccaat aaatagagcg acaggttggt acatgaaatc cgtctggttg ctgtagcaaa
    34141 gacacgagta tataagcgaa agcaagatgg gtaaacgtct gaactgtaag tgatcgtcag
    34201 ggctaatctg aaaagccccc gtaaatcaat cggctaaatt gccatatttt atcatcaatc
    34261 ttgccgcctg atcttaatct gaaaaatcat ccaatacaga cagataccca ttacacctcg
    34321 ggtcataacc agcaggatca accgcatgta aattaaaaaa gagcgtgttg ttctgatgaa
    34381 acaaaaagag ctatggatta accagatcaa agggttatgt atttgtctgg tggtgattta
    34441 tcactcggtc attacctttt atccgcatat gaccactttt cagcatccgt tatcggaagt
    34501 cctgagcaaa tgctggatct atttcaatct ttaccttgcc ccctttcgta tgccggtttt
    34561 tttctttata tccggctatt tgattcgccg ctatatcgac agcgtaccgt ggggaaattg
    34621 tctcgataaa cggatctgga acatcttctg ggtgctggcg ctttggggcg tggtgcagtg
    34681 gctggcgtta agtgcgctga accagtggct ggcacctgag cgcgatttaa gcaatgcctc
    34741 caatgccgct tatgccgatt ccaccggtga gttcctgcac gggatgatca ccgccagcac
    34801 cagcttgtgg tatctgtatg cgttaattgt ctatttcgtg gtatgtaaaa tttttagccg
    34861 tctggcgctg ccactattcg ccttgtttgt actgctgagt gtggcggtta atttcgttcc
    34921 cacgccgtgg tggggaatga acagtgtgat acgcaatttg ccttattaca gccttggcgc
    34981 atggtttggc gcaacaataa tgacctgtgt taaagaggtg ccgttgcgcc gccatctgct
    35041 gatggcttct ttgcttaccg ttctggcggt cggtgcctgg ttgtttacta tctcgctgct
    35101 gttgtcgctg gtatcgattg tggtgatcat gaagctgttt tatcagtacg agcaacgatt
    35161 cggtatgcgc tccaccagcc tgctgaatgt gattggctcc aacaccattg ctatctacac
    35221 cacccatcgc attctggttg aaatattcag cttaactctg cttgcgcaaa tgaacgcagc
    35281 acgctggtcg ccgcaagtcg aactgacact cctgctggtt tatccctttg tcagtttgtt
    35341 catctgtact gttgcgggct tgctggtaag aaaactttca cagcgtgcat tcagcgatct
    35401 gttgttctcc ccgccttcac tgcccgctgc cgtcagttac tctcgttaaa ccatcacccc
    35461 agccggggcg attcaatttg ctaaaccatg tcgattacgg ataatgaaca gcattgctta
    35521 tcagagatat gcccccatat gttgaggcat atcctaacga gaatctgaca accgttatgc
    35581 ctgaacaata tcgttatacg ctgcccgtca aagcgggtga gcagcgtctg ctgggcgagt
    35641 taaccggcgc agcctgtgca acgctggtag cggaaattgc cgaacgtcac gccggtccgg
    35701 tggtactcat tgcaccagat atgcaaaatg ctctgcgttt gcatgatgaa atcagccagt
    35761 tcaccgatca gatggtgatg aatctggcgg actgggaaac tcttccctac gacagttttt
    35821 cgcctcatca ggacattatc tcctcgcgcc tttccaccct ttaccagcta ccgacgatgc
    35881 agcgtggcat actgattgtt ccggtgaata cgcttatgca gcgcgtttgc ccacacagtt
    35941 ttctccacgg tcatgcgctg gtgatgaaaa aaggtcagcg cctgtcacga gatgcattac
    36001 gaacccaact ggacagcgcc ggttatcgcc atgttgacca ggtgatggag cacggcgaat
    36061 acgccacgcg cggcgcgttg ctggatctct tcccgatggg gagtgagctg ccttatcgtc
    36121 ttgatttctt tgatgatgaa atcgacagcc tgcgggtgtt tgacgtcgac agccagcgca
    36181 cgctggagga agtagaagcg atcaatctgc tgcccgcgca cgaatttccg accgataaag
    36241 cggcaattga actgttccgc agccagtggc gcgatacctt cgaagtgaag cgcgatccgg
    36301 aacatattta ccagcaagtg agtaaaggca cattacctgc tgggatcgag tactggcagc
    36361 cattgttctt cagcgaacca ctgccaccgc tgttcagtta tttccctgcc aataccttgc
    36421 tcgtgaatac tggcgatctg gaaaccagtg ccgaacgttt ccaggctgac acgctggcgc
    36481 gttttgagaa tcgcggcgtc gatccgatgc gcccgctgtt gccaccacaa tcgctctggc
    36541 tgcgggtgga cgagctcttc tcagagctga aaaactggcc ccgagtgcag ctaaaaactg
    36601 aacatttacc gacaaaagcc gcgaatgcca atttaggttt ccagaaactg ccagacctgg
    36661 ccgttcaggc gcaacaaaaa gcgccgctgg atgcgctgcg taagttcctc gagactttcg
    36721 acggtccggt ggtgttctcg gtagaaagtg aaggtcgccg tgaagcgctg ggtgaactgc
    36781 tcgcacgaat taaaattgct ccgcaacgca ttatgcgtct tgatgaagcc agcgaccgtg
    36841 ggcgttatct gatgattggc gctgccgaac atggttttgt cgataaggcg cgtaatctgg
    36901 cgctgatctg cgaaagcgat ctgctcggtg aacgtgttgc ccgccgtcgt caggattctc
    36961 gccgcaccat caaccccgat acactgatcc gtaaccttgc ggagctgcat attggtcagc
    37021 cggtggtcca tctggagcac ggcgtcggtc gctatgccgg aatgaccacg ctggaagctg
    37081 gcggcattac tggcgagtac ctgatgctca cctatgccaa cgacgccaaa ctgtatgttc
    37141 cggtgtcgtc actgcatctg attagccgtt acgcaggtgg cgcggaagaa aacgccccgc
    37201 tgcataaact tggcggcgat gcgtggtcac gcgcgcggca gaaagcggcg gaaaaagtgc
    37261 gtgatgtggc ggcggaattg ctggatatct acgcgcaacg cgccgccaaa gagggcttcg
    37321 cgtttaaaca cgatcgtgag cagtatcagt tattctgcga cagcttcccg tttgaaacca
    37381 cgccggatca ggcgcaggcc attaatgcgg tacttagcga catgtgtcag ccgctggcaa
    37441 tggatcgtct ggtgtgcggc gatgttggct ttggtaaaac agaagtggcg atgcgcgccg
    37501 ctttcctggc agtagataac cacaagcagg tagcggtgtt ggtgcctacc acccttcttg
    37561 cgcagcagca ttacgacaac ttccgcgacc gtttcgccaa ctggccggta cgtatcgaaa
    37621 tgatctcccg tttccgcagc gccaaagagc agacgcaaat ccttgcggaa gtggcggaag
    37681 ggaaaatcga tattctgatc ggtacgcaca aactgctgca aagtgacgtc aagtttaaag
    37741 atttaggcct gctgattgtc gatgaagaac accgcttcgg ggtgcgtcat aaagagcgca
    37801 ttaaagcgat gcgcgcgaac gtggatattc tgacgcttac tgcaacgccg atcccacgta
    37861 cgctgaatat ggcaatgagc ggaatgcgtg acctgtcgat tatcgccacg ccgcccgccc
    37921 gtcgtctggc agttaaaacc tttgtccgtg agtatgacag cctggtggtc cgggaggcga
    37981 tcctgcgtga aattttgcgc ggaggacagg tttattatct ctacaatgat gtggaaaaca
    38041 ttcagaaagc cgccgaacgg ctggcagaac tggtgcctga agcacggatt gccatcggtc
    38101 acgggcagat gcgcgagcgc gaactggaac gggtgatgaa tgatttccat catcaacgtt
    38161 tcaacgtgct ggtttgtacc accattatcg aaaccgggat cgacatcccg acagccaaca
    38221 ccattatcat tgaacgtgcg gatcacttcg gtctggcgca gctgcaccag ttacgcggtc
    38281 gcgtcggacg ttcacatcat caggcatatg catggctgct gacgccgcat ccaaaagcga
    38341 tgactaccga tgcacaaaaa cgtcttgaag cgattgcctc gctggaagat ctcggggcag
    38401 gttttgcgct ggcaacgcac gatctggaga ttcgcggcgc gggtgaactg cttggcgaag
    38461 aacaaagcgg ctcaatggaa accatcggtt tctcgctgta tatggagttg ctggaaaacg
    38521 ccgtcgatgc actgaaagcc ggacgcgagc cgtcgctgga agatctcacc agccagcaaa
    38581 cagaagtcga gctgcggatg ccgtcgctat tgccagatga tttcatccct gacgtgaaca
    38641 cgcgtctgtc gttctacaaa cgtattgcca gcgccaaaac ggaaaacgaa ctggaagaga
    38701 tcaaagtcga gcttatcgat cgcttcggcc tgctgccaga tccggcgcgt accctgctgg
    38761 atattgcccg tctgcgccag caagcgcaga aactggggat caggaagctg gaaggtaatg
    38821 agaaaggcgg ggtgatcgaa tttgccgaga agaatcacgt taatccggcc tggttgattg
    38881 gtttgctgca aaaacagccg cagcattacc gccttgatgg tccgacgcgc ctgaaattta
    38941 ttcaggattt gagtgagcgg aaaacgcgta tcgaatgggt acgccagttt atgcgtgaac
    39001 tggaagagaa cgcgatcgct taatgtaaat cgggggcgtt tctgcgcccc catctgcaac
    39061 atttacaaat tctttgcact ttcctgcact atccgacact gtcaccatcc ataattcagg
    39121 cttatctgtt tattacaata accttatatt tattatgtat ttttggtgat gatcaaaacg
    39181 cgtttttctc gctggctaac gttttttacg ttcgccgctg ccgtggcgct ggcgctaccg
    39241 gcaaaagcca acacctggcc gctgccgcca gcgagcagtc gtctggttgg cgaaaacaaa
    39301 tttcatgtgg tggaaaatga cggtggttct ctggaagcca tcgccaaaaa atataacgtc
    39361 ggctttctcg ctctgttaca agctaacccc ggcgttgatc cttacgtacc gcgcgcgggc
    39421 agcgtgttaa cgatcccgtt gcaaacccta cttccagacg cgccgcgcga aggcattgtg
    39481 atcaacattg cggagctgcg tctctattac tacccgccgg gtaaaaattc ggtaaccgtg
    39541 tatccaatag gtattggtca gttaggtggt gacacgctga caccgacaat ggtgaccacc
    39601 gtttcagaca aacgtgcaaa cccaacctgg acgccaacgg caaacatccg cgcccgttat
    39661 aaagcacagg gaattgagtt gcctgcggta gtgccggctg gaccggataa cccaatgggc
    39721 catcatgcga ttcgtctggc ggcctatggc ggcgtttatt tgcttcacgg tacgaacgcc
    39781 gatttcggca ttggcatgcg ggtaagttct ggctgtattc gtctgcggga tgacgatatc
    39841 aaaacactct ttagccaggt caccccaggc accaaagtga atatcatcaa cactccgata
    39901 aaagtctctg ccgaaccaaa cggtgcgcgt ctggttgaag tacatcagcc gctgtcagag
    39961 aagattgatg acgatccgca gctgctgcca attacgctga atagcgcaat gcaatcattt
    40021 aaagatgcag cacaaactga cgctgaagtg atgcaacatg tgatggatgt ccgttccggg
    40081 atgccggtgg atgtccgccg tcatcaagtg agcccacaaa ctctgtaaat cctggataaa
    40141 aaaaatcccc gcgacacggc atgtcgcggg gaaaaaatca gcagtagcag gcattaatga
    40201 gggttaatgc ttatttataa attactgctg ttccatggag ggtattcgga ccggttacag
    40261 aagtaatacg gaaagatttt gcgcccatct catccgcttt ttgcgccagc tgctcttcca
    40321 gcgatcccag atttgtcccc gcgttagcac tgatagtacc gactttttgt tggccttctg
    40381 gcgttgactg aacttcgaca gccgcaaagt tggcaaatga catggagctt aaaatcgccg
    40441 cagcgatgag gtttttacgt ttttcataat agtggcctta tgcagatgaa tgacgtcgca
    40501 agaaattact taacgatcga taactaaatg atagatgtga tctggatcac atacaagata
    40561 ttttttataa caatcattaa ttaatttaaa aatctttaaa tttcagtcaa atagaaagca
    40621 attattttta tgggatctgg actggtgaac ggaggtgatt atttttataa taacctttca
    40681 ttcacagtca ccaggtacaa cggcatcatg gcaactgact caacacaatg tgtaaaaaaa
    40741 agccgtggcc gcccaaaagt gttcgacagg gatgccgcgc ttgataaggc catgaaattg
    40801 ttctggcaac acggttatga agcgacttct cttgcggacc tcgtcgaagc aaccggagcc
    40861 aaagcgccca cgctatacgc ggaatttacc aacaaagagg ggttatttcg tgccgttctc
    40921 gaccgctata tcgatcgttt tgccgctaag catgaagcac agctgttttg tgaagagaaa
    40981 agcgtggagt ctgcgctggc tgactatttt gctgccatcg ccaactgctt taccagcaaa
    41041 gacaccccgg caggctgctt catgatcaac aactgcacca ccctctcccc agattcagga
    41101 gatatagcca atacgttgaa atcacgccat gcgatgcaag agcgcacttt gcagcagttt
    41161 ttatgtcaac gacaagcgcg cggggaaatc ccgacccact gtgacgtgac acatctggca
    41221 gaattcctta attgtattat tcaggggatg tcgatcagcg cacgcgaagg tgcatcgctg
    41281 gaaaaactga tgcagattgc cggaacgact ttgcgtttat ggcccgaact ggtgaaataa
    41341 gtcgcaaaaa aggcccctca gcctgagcga atgaggggcc aaattgcaga gtacagcctt
    41401 gttatacttt gttattcaaa atcagttgcc cgttactatc tagcgggatc tgcgtacccg
    41461 gatcgcggtc catgcggatt ttgccctgct gatcgccaat cttataggtc acatcataac
    41521 cgagcatttt ttctgacttg tcatacaccg ttttacaacg ctgttgcgta gtcgtgtaag
    41581 tatcgctttc ctggagagag ccctggatct ggttaccggc atatccacca cccagcgccc
    41641 ccacaacagt ggcgacatct ttaccgcgac caccaccaaa ctgatgccct atcacgccac
    41701 cagcaacagc gccgagcacc gacccggtaa tgcgattttc atcctgcacc ggtcgacgat
    41761 gggtcactgt gacgttgcga cactcctgac gcggtgtttt aaccgtttcc ttgattgggg
    41821 ttgcagaaac aacctgagcg tattgcgggc cccgttcaaa cacgttcaga ctggccactg
    41881 ccgctacgcc caacgcagct gcgacaccaa tcccgatacc cgccaacatt gatttattca
    41941 cgggaacacc tccttctttg cagttatgcc aatcatgcaa gcgaaggaga aaagcagcta
    42001 tcagacagaa gatgaaaagc gcgggaatga ctggaaatac ttaaggattc ggaggagtct
    42061 gaaggaatgc ctgatgcgac gcttaacgcg tcttatcagg cctaccgctc gtaccatttg
    42121 tccagggcgg ataaggcgtt tataccacat ccgccagtgt acgtcgatta atgcaacttc
    42181 aaacgcggac ggataacgcg gttaatactc cccaccagca tcattaatcc ggttttaaag
    42241 taaccatgca gcgcaatctg atgcattcgg tatagcgaga tatatacaaa gcgcgcaatt
    42301 cgtccttcaa tcatcattga gccgcgcgtc aggttaccca tcaggctacc aacggtggag
    42361 aagttcgaca gcgataccag cgaaccatga tctttatact gataattttt cagcggctta
    42421 ccgttcatct gcgccagaat gttgttcatt gcgcaagtcg ccatctggtg tgcagcctga
    42481 gcacgcggcg gaacaaagcc cccttccgga cgcgggcatg acgcgcagtc gccaatagcg
    42541 taaatgtctg gatcgcgagt ggtttgcagc gtcggttcca ccaccagctg gttgatacgg
    42601 ttagtttcaa gaccaccgat atctttcagg aagtctggcg ctttgatccc ggctgcccat
    42661 accatcagat cagcctcaat atattcgcca tctttagtgt gcaggccgcc ttcatcagca
    42721 ctggtgacca tggtttgcgt cagcacgcga acgccaagtt tcgttagctc gttgtgggcc
    42781 gcagcagaga tacgtggcgg taatgcaggc aaaatacgtt ctcccgcttc taccagcgtt
    42841 acgttcaggg cttcgttggt caggcctttg taaccgtagc tgtgcagttg cttgatcgcg
    42901 ttgtgcaatt cagcggagag ttctacaccc gtcgcgccgc cgccgacaat cgcaatgttc
    42961 actttgccat ttgcgcccag gttggcagag tatttcagga acagattcag catctcctgg
    43021 tggaagcgac gcgcctggtg cgggttatcg aggaaaatgc agttctcttt gacgcctggc
    43081 gtattgaaat cgttagaggt gctacccagc gccatgacca gggtgtcata ggcgatttta
    43141 cgttccggaa ccaacagttc acctttctcg tcgcgtagtt ctgcaatagt gattgttttc
    43201 gcttcacgat caatatcaat gacggaaccc aactggaact ggaaaccatg attgcgcgca
    43261 tgggccagat agctcaacgc atcgacgcct tcatcaagcg agccagtcgc cacttcgtgc
    43321 agcagtggtt tccacaggtg gctgtggtta cgatcgacca gcgtaatttt ggcttttttc
    43381 ttacgtccca gcttatgccc cagctgtgtt gccatttcca gcccaccagc accgccgccg
    43441 acaatcacaa tctttttcaa tggcgtagtc aacgtgcccc ccttaaattt attaaccaat
    43501 tgttaattaa tggttattaa catagctctt aattaacaac aggttacagc aatgaaaatg
    43561 ttcttcgcta ttgagaataa catgaatggt gcattggtca taccaaaatt gatgttaatc
    43621 aagttttgtt gcttaaaaga taggcaactt taccggggga gatcaaaaaa tagagtttat
    43681 tgtttattat ttgagcaaat tcggaagggt aatatagggt gaagcgtata tcgagggcgt
    43741 ttggtcgtcc tgataagatg cgcagcatcg catcaggcat tgatgctcga ctgtcggatg
    43801 cggcgtggac gccttatccg acttacagaa ttcggagcgc atcaggtacc ggcaaggtta
    43861 cctgatgcga cgagggcatt tacccgaggg ttttgaacgc tttaatgcgc tgtaaatgcg
    43921 gggagatatt cttgaatttg tgcgtctgct cttcgtccca gacaatctcg taataatgat
    43981 gcaactcttc agatgtccgc tggctgttaa gtgcttcatc attacgcgac aaaataacca
    44041 gacaacgatc gcgattcttc tcgcggaagt tggtcacaca cttagtggca atatcggcat
    44101 actcttccgg gcgatcaatc ttgccttcca tgttctcata agggaacaaa ttagggttga
    44161 agatcacctg gcggatatcg caaagaaaac caatccgttc cgcccagtat ccgcccaggc
    44221 caacgccgca aattagcgga cgctcgtcaa cgttcagttg caacattttg tccacttctt
    44281 taagcagatg ctgcatatca tgtttcggat gccgcgtact gtagcttatc aagcgtacat
    44341 ccgggtcaat aaactgcaat tgtaagactt tctcgtggtt acccggactg ttagagtcaa
    44401 aaccgtgtaa atagataatc atcgcttcct cacataagcc agggttagtg acctactttc
    44461 tcttcctgcc agcgttcatg taactgattc agacgggtgc tgatcgcttt ccagcgagcc
    44521 gagtccatca gttcctgtcg cgaaaatgaa cctttatgat acaaacgtgt aacacgttct
    44581 gccttgatcg gtgacagatt atctaacacg ctgacggccc ctttacgatt attgcagacc
    44641 aggatcatat cgcaacccgc atccagtgat gcctgcccgc gttcggcata actgcccata
    44701 atcgcggcac cttccatcga taaatcgtca gagaaaatca cgccgtcaaa ccccagttcc
    44761 tgacgcaaaa cggttttcag ccagtaggga gaaccgctcg ccggacgcgg atcaacatca
    44821 ctgtagatca catgcgcagg cataatggcg tcgagtttat tttcgcggat taaggaactg
    44881 aagaccgaca tatctttagc gcgaatctcc gcttgtggac gtggatcgca cggtgtttct
    44941 ttgtgtgagt ctgccgttac tgcaccgtgt cctgggaagt gtttcccggt cgttttcatt
    45001 ccggcttcat gcataccatc aataaaccgg ctggcaattg ccagggcttt ttgtggatcg
    45061 gcatgataag aacgctcgcc aatcgccgcg ctgatatgcc cgacatccag cactggcgca
    45121 aagctgatat caatatccat agcgatcatt tcgctggcca tcagccaacc cgcctcttgc
    45181 gccagtttgc cgccctcttc cattcccaac agcgcagcaa aggattgtgc tgccggtaag
    45241 cgggtaaaac cttcgcgaaa acgctgcacg cgtccacctt cctgatctac cgccaccacc
    45301 agatgattgc gcgatgctgc gcggatctgg cgcaccagtt cacgtaactg ggcaggatca
    45361 tgatagttac gcgtaaagag aatcagccct cccaccagcg gatgcgccag tatttcacgc
    45421 tcttccgcgt ccagttcgta accttcgaca tccaacatta ctggacccac actgctctcc
    45481 ttattgtttt attaatagct gccgccaggt gtcatcggcc agcctgataa attgttgatc
    45541 gccggtttgt cgccagcggt actcaaacca ccctcctttg agcatcagca gccagggaaa
    45601 ccatcgcctg acctgacgcc ataattgcgc cggataaatc ttcgcgcgag tggcatagtc
    45661 attgaccaat tgccggtgct gttcagtatt ttccacccac accgccgcca gttccagcgc
    45721 gatatcacca tctccagcat actcccagtc gatgagtttt aaccctgatg cgctatgcac
    45781 taaatttccg gcgtggacat ccatatgcaa tggacttaag cgtaaaggtc gtggttcccg
    45841 cgctttgcgc agacgtttta acattcgcag ccaacccaca gtccgccgcg ccggatcgct
    45901 ttgctgccag tacagttcca gtaagggcaa cagcgttatt cgccagccaa aacgtggttg
    45961 ttgatgtaga taatacagca agcctgccag ttcgttggta tccggcaaat acgtttttac
    46021 cgcaccgggc agatagtcga ctaccatcca gtcacggaga tataaatgcg gcttcggtgc
    46081 aatgcttgcg ggtagttgtg ataaagcccg atactggcgc aagaacgcgg actgcggcgc
    46141 atcaggatcg tgcggctgac gcacaacaaa acgctggccc tgatgttcaa tgagaaaact
    46201 accgccacta agcccactat taaacgtcgt gacgggatga aactgcggga aaaagcgcga
    46261 cagcaattcg tcgcgcgtga tgggattatt gctgcgaaac ggcaccttta cctgaccaga
    46321 taatttcgcc cgtctgcacc agcatcagct gcatttgtag ggtcggagcg ttaacgttgc
    46381 cagaggcgca ggagtacagc acgtaatgag cgccgacatt gcgggcaatg cctatggctt
    46441 tactacgggt gcctaaactg tcctgcggcg acaaacctaa ctgttgctta gccatcgaca
    46501 gttgctgggc ggaaaccagg gtaaatttcc cgttattagc cagcgcattt cgcagcgttt
    46561 cggtcgcttc tgcggcattc agcgaaccgt tagtacggtt gttaacgcta tcaaccagca
    46621 ggacgctacc cgcagtcacc ccgtcagccc caagcatctt actgaccatc ggctgcattg
    46681 cgccattcca gtcataatgg cgaatatgcg gcgcaggcgg tgccgttcga tcttcgtgct
    46741 caattgggcc tggctgctgc gggatcgtcg gcaccgaggg cactgtgggg acaggctgtt
    46801 gtggctcggc tggttgttcc ggcgctggtt tcacttcttc taccggtgca ggttcacgtt
    46861 gccccacaca cccggcgaga aacatcgcca gcgcggtaat caaggcgtag cgactcattt
    46921 ttgtcatcaa gattcacccc tttacaaata tagataaagt ctgactttgt gcgcccccag
    46981 aaaattggcg ctgccgtaca gcgttaccgc cgaatgtgcg ggaatggtaa cgctgcgtgg
    47041 cctttccaga ggatgcatct ccagccctct ggcgtcatac cagtaaaaac gataatgaac
    47101 ggtaacgggt tcttgccttt cgttataaat cgttgaggat gctgaaggtt gaatatcaga
    47161 cgtcgaaagg acgggctttt ctgcactgat gcccgcagcc aataaagatg actccatcac
    47221 cagcgattgc tcatcattca ccggaatttc cggatgtgaa cgacagccca ccagcagtaa
    47281 caacaccaga gacaccagcc caaagcatcc ttttctcatc gttacagacc tttatgcgcc
    47341 agcattggtc ccagcggacg gccacccaac aagtgcatat ggatgtggta aacctcttgt
    47401 ccgccatggc ggttggtatt catgatcaga cgatagccat cttcggcaat accttcttgc
    47461 tcagcaattt ttgccgctac ggtgatcatg cgtcccagcg cctgctcatg ctcagctgac
    47521 acgtcgttca cagtcggaat gagaatattc ggaatgatca gaatatgcgt tggcgcttgc
    47581 ggcgaaatat cgcgaaacgc cgttaccaga tcatcctggt agacgatatc ggaggggatc
    47641 tcacgacgaa taattttgct gaatatagtt tcttctgcca cgacattttc ctttttcaaa
    47701 tagccatgcg ttgcgccatg ctacgccgcg ccacactgcg agtatagagt atggtcgagt
    47761 taaacctgct ctttcaatct taactcacga tttcttaagc aaaaaatcgt tttgctgccg
    47821 tatatatcgc cattattccc atttctgcct gcgaatgcgc tgaaaagaca ttctctgttt
    47881 ggtttacacg acaaatatga atgcgtatat ttctcatttg catttgcagc aaaattattc
    47941 gcacactaaa tataaccgct catcaccctc ctccggcatg agcctgacga caacataaac
    48001 caagagattt caaatgcttt caacacaatt taacagggat aatcaatatc aagtcatcac
    48061 caaaccgtca ctacttgccg gttgcatagc actggcacta ttaccttctg ccgcttttgc
    48121 tgcaccagcc actgaagaaa cggtgattgt tgagggttca gccacagctc cagatgatgg
    48181 cgaaaatgat tacagcgtaa cgtctacctc tgcgggtacc aaaatgcaga tgactcaaca
    48241 tgatattcct cagtcggtca ctattgttag ccagcagcgg atggaagatc agcagttaca
    48301 aacgctgggc gaagtgatgg aaaacacgct ggggatcagc aaaagtcagg cggattccga
    48361 tcgtgctctt tattattccc gcggattcca gatcgataac tatatggttg atggtatccc
    48421 cacctatttt gaatcgcgct ggaatctggg cgacgcactt tctgatatgg cactgtttga
    48481 acgcgtagaa gtagtgcgtg gcgcgacagg actcatgacc gggacgggta atccatctgc
    48541 ggcaattaat atggttcgaa aacacgcgac ctgtcgtgaa tttaaaggcg atgtctcggc
    48601 ggaatacggt agctggaaca aagaacggta tgtggcggat ttacaaagcc cactcaccga
    48661 agacggtaaa atccgcgcgc gaattgtcgg cggctaccag aataacgact catggctgga
    48721 ccgctacaat agtgaaaaga ccttcttctc gggcattgtc gatgctgatt taggcgatct
    48781 tacgacgctt tcagccggtt acgaatatca gcgcattgat gttaatagcc caacctgggg
    48841 cggtttaccg cgctggaata ctgatggcag cagcaacagt tacgatcgcg cacgcagtac
    48901 cgcacctgac tgggcgtaca acgataaaga gatcaacaag gtctttatga ccctgaagca
    48961 gcggtttgct gatacctggc aagcgacact gaatgccacc cactctgaag tcgaatttga
    49021 cagcaaaatg atgtatgtcg atgcctatgt aaacaaagcg gatggtatgc tggttgggcc
    49081 atacagtaat tatggacctg gctttgatca tgtcggcggc accggttgga acagtggcaa
    49141 acgtaaagtt gatgcgctgg atttgttcgc tgacggtagt tatgaattgt ttggtcgtca
    49201 gcacaatcta atgtttggtg gcagttacag caaacaaaac aatcgttcag ttcatgggcc
    49261 aacatcttcc cggatgaaat tggcagtttc tacaacttta atggcaattt cccacaagcc
    49321 gactggtcat cacagagcct ggcgcaggac gataccacac atatgaaatc gttatatgct
    49381 gccactcgtg tcacccttgc cgatccgctg catctgatcc tcggcgcacg ttataccaac
    49441 tggcgggttg atacgctgac ttacagcatg gagaaaaacc acaccacgcc ttacgctggt
    49501 ctggtgtttg acatcaatga caactggtcg acctacgcca gctatacctc tattttccag
    49561 ccgcaaaatg atcgtgacag ttcaggcaaa tatctggctc caatcaccgg taacaactac
    49621 gagctgggtc tgaaatcgga ctggatgaat agccgtctga ccaccacgtt agccatcttc
    49681 cgtattgagc aggataatgt cgctcagtcc accggtacac ctatccccgg cagcaacggc
    49741 gaaaccgcct ataaagcggt ggatgggaca gtcagtaaag gggtggaatt tgaactcaac
    49801 ggcgcaatta ccgacaactg gcagctgaca tttggcgcaa cgcgctatct tgcagaggat
    49861 aacgaaggaa acgccgttaa tcctaatctg ccacgcacca cggttaaaat gttcaccagc
    49921 tatcggttgc ctgtcatgcc agagttgaca gtcggcggtg gtgttaactg gcaaaatcgc
    49981 gtgtataccg acaccgtgac accgtatggc accttccgcg ccgagcaagg tagctacgcg
    50041 ctggtggatc tcttcacccg ctaccaggtg acgaaaaact tctcgttaca ggggaacgtc
    50101 aataacctgt tcgacaaaac ctacgatacc aacatggaag gttctatcgt ctacggcgca
    50161 ccgcgtaatt tcagcattac cggcacgtat caattctgaa taacacctgt aaaaaaggca
    50221 gccatctggc tgccttagtc tccccaacgt cttacggatt agtggttacg gatgtactca
    50281 tccatctcgg ttttcaggtt atcggattta gtaccgaaaa tagcctgaac accagaacca
    50341 gcaaccacta cgcccgctgc gcccagtttc ttcaggccag cctgatccac tttagacaca
    50401 tcagcaacgc tgacgcgcag acgggtaata catgcgtcga ggttagtaat gttttcttta
    50461 ccaccaaatg cagcaaccag agccggtgcc atttcgctgg tacctgttgc ttttgcatct
    50521 tcagtcgcgt cttcacgacc cggcgttttc agatccagtg ctttaatcag cacgcggaag
    50581 atggtgtagt aaacaatcgc ataaccgata ccgacgatcg gtaacagcca cagtttgctg
    50641 ctgttaccag acagaacgat gaagtcgatc agaccgtgcg agaacgacgt accgtcacgc
    50701 atccccagaa gaatacagat tgggaatgcc aggcctgcca gaatcgcgtg gatgatgtac
    50761 aggatcggcg caacgaacat gaagaagaac tcgatcggct cggtgatacc ggtcaggaac
    50821 gaggtcagcg ccgcggagat cataataccg cccactttcg cgcggttttc tggtttagca
    50881 gagtgccaga tagcaattgc ggcagctggc agaccgtaca ttttgaacag gaagccacca
    50941 gacagtttac ccgcagtcgg gtcacccgcc atataacgcg gaatgtcgcc gtggaaaacc
    51001 tgacctgctg cgttggtgta ttcaccaatc tgcatctgga aaggtacgtt ccagatgtgg
    51061 tgcagaccaa acggtaccag gcaacgttcg atgaaaccgt aaatgccaaa cgcaactacc
    51121 gggttctggt aagcagccca ctgagagaag gtctggattg cagaaccaat cggcggccaa
    51181 atgaaggaca gcacaacgcc agtaaagatg gcagccaggc cagaaatgat cggcacaaag
    51241 cgtttaccgg caaagaagcc aagatactca ggcagcttaa tacggtagaa acggttaaac
    51301 atgtacgctg caatcgcacc ggagataatc cctccgagta cgccagtatc cgccaagtgt
    51361 ttagaggcga tttcttcagc aggtaaatgc agtaccagtg gcgcaaccac ggccatggtt
    51421 ttaaccatga tgccataggc aacaactgcg gccagcgcgg atacgccatc gttattggta
    51481 aagccgaggg cgacaccgat cgcaaaaatc agtggcatgt ttgcaaagac ggaaccgcct
    51541 gcttctgcca taacatgcga tacaacggcg ggcagccagc tgaaattcgc ggaaccgacg
    51601 cccagcagaa tacctgcgat aggtagtacg gataccggca gcatcagcga tttaccgacc
    51661 ttttgcaggt tagcaaatgc attcttaaac ataattgaga gtgctcctga gtatgggtgc
    51721 tttttttacg ttctcacgcg tggcaagggg ggagagcctc gccgtgtaca gggcatctaa
    51781 gcgcccttta tttattacac agagtaaaat aattcagtgc caatttgttt gacggctatc
    51841 acgtttcaac ttcagatcat tggagaagtt gcacattttg acaaaacata tgtcacaata
    51901 tttcaaacgc ggttattcac cgcccaattt tcggcggtga actttactcg ttttagccat
    51961 taattacgag ctttaaaaaa actcattcaa cggatggatt gaaggcggga agcgtcgatg
    52021 tgaaacagac gggcgaagtt atcggtggtt acctgcgcca gttcttcaac ggcaacacct
    52081 ttcaacacag ccatgtattc tgcaacgtca cgaaccatcg caggttgatt ctcttttcct
    52141 cgatgcggta ccggcgcaag gtaaggtgag tcagtttcca ccagtaatcg atccaggggg
    52201 acataacgcg cagcatcgcg cagttgctcc gcattacgga aggtcacaat gccggaaaag
    52261 gagatgtaaa atccgagatc cagtaattta cccgccgttt ctctgtcctc tgtaaaacag
    52321 tgtagtacgc caccgcaatc cgtcactttt tcttcgcgca gaattgccag cgtatcggca
    52381 cgggcgtcac gggtatggac gataaccggc ttgttcagtt cacgaccaat ctggatatga
    52441 tggatgaagg actcttgctg acgtacttta gtttccggtg tgtaataata atccagcccg
    52501 gtttcaccca gcgctacaac accctcttct gccgccagac ggcgtaaatc ttctacatcg
    52561 tagggatcat tctggttgag tggatgcacg ccacaagaaa atacgacgtt gtcacgttcg
    52621 cctaccagat cccgcatatg taaataaccc ggtaatgttg tggcgactgc cagacaaaat
    52681 ttcacatcgc gtgcggcggc tttcgccaga acgtcatcca cgtccttatg caaagattca
    52741 taatccagac catcgagatg gcagtgtgag tcgactaaaa acatgatgtc tctcttaaag
    52801 atgaggaacc ggtaacacaa cgcccggttg caggtaatgc tcaatacgca gtaaaagatc
    52861 ggtgatgaga agctcgcggt tgatgcctgt gacagacatt aactgttcac gaatgtggca
    52921 aacatccccc agtatagcct gcaggcacga gggagaaaga tggtttgcca gttcggcgac
    52981 caggcccggc acatcaacat tggtcacctg cgcagcgcca tgatggcgtt ttagcgcatc
    53041 catcagcaac gttgccagcc agtgtaaacg cgccggagct tgttcatgat taagggccgc
    53101 cagcagcgaa taccagtcgc ccgatggcac gctatatgcc aacgcctgac acaatgtttc
    53161 acgagcctgc cagttatctc cctgaaacaa cgccagtgcc gcgccaggcg aaccggcgct
    53221 taagcgcaat gcggcaagta atgcatcctg tgacattgtc acttcgcgtg aaagccaagt
    53281 cacggcgtac tgttctggag gcggcgcaag gtaatgtaac cgacaacgac tacgtaatgt
    53341 tgccagtaaa cgttcaggct cgcgggtagc caggaaaaac caggtttctg ctggtggctc
    53401 ttcaagcgtt ttcagcaatg cgttagccgc ggcgtcggtc agtaaggcag catcggttac
    53461 ccagacaact ttcgcaccac ctaggcgtgc gtgctcattc agcttttcgg tgacctcacg
    53521 taccgcatca atgcccagcg tatttttccc tttttcggga gccagggtgt agtaatcggg
    53581 atgcgtgcca gcctgcatca actgacatcc acgacagtga ccgcaacttt tgtggccctg
    53641 cggttgttgg cagagtaaat aacggctcag ggcgtagatt aaagcatcat cgcccatgcc
    53701 cggtaacgcc tgaatgagta gcgcatggtg acctcttccg gcctgatagc tggctaccag
    53761 tttttcgaaa tcaggtcgta accatggata ccatctcatg cgtccaactc cttcacccag
    53821 tgggtcacgg tagtgcggat tgcatccatc acggcctcca gcggctgggt ggcatcaatg
    53881 gtatgaatac ttttatcttg tgcagccagt tccagatagc gggcgcgggt gcgattaaag
    53941 aaatcgaaag actcttgctc aatacgatcc agctcgccgc gcgcacgcgc gcgttttaag
    54001 ccaacttccg gggtaacatc gagatagagc gttaagtctg ggcgaaaatc cccgagaaca
    54061 gcatcacgca gtgttgccag catatgttgg tcaataccac gtcctccgcc ctgatacgcc
    54121 tgagtggaaa gatcgtggcg atcgccaatc acccaggtgc cgttagccag cgcaggtttg
    54181 atgaccgttt ctaccagttg aacgcgcgcg gcataaaaca tcagaacttc ggctttatcg
    54241 gtaatgactt catcgcctac cgatttgata tccagcacca ggcttcttaa cttttcggca
    54301 agttgcgtac cgccaggttc ccgagtgaaa accatgtcgc ggatacccag ttgctcgagc
    54361 gtctcaacca ccacattacg cgcggtagtt ttgcctgcgc cttccagccc ctcaatgacg
    54421 atatacttac tgcgcatttt tttccttaag cactttcaga taatcctgca cagacttgtt
    54481 atgactggca agattggtat taaacgtgtg accaccttta ccatcggcca caaaatagag
    54541 atacggcgtt tttgccggat gcgcagcagc cttcagcgaa tccgcccccg gcgtagctat
    54601 cgcacccggc ggcaaaccgg taatggtata ggtgttatac gctgtcggcg tttccaggtc
    54661 tgcacgagaa agtttgccat tataacgctc tcccatcccg taaatcacgg ttgggtcggt
    54721 ctgcaggcgc ataccaatgc gtaaacggtt gataaatacc gaggcaacct gatcgcgttc
    54781 actggcaacg gcggtttctt tttcgataat tgatgccatc gtcaccagct gatttttatc
    54841 tttataaggc agaccgtccg cacgcccttc ccaggcgcta tcgaccgctt tcaccatttt
    54901 cttgtgaact cgcttgagta acgcgacatc ggtggtattg gcggtataca tcaaggtgtc
    54961 tggccagaac caaccttcaa tccactccgg gttttccagt tcaagtgcct gcgctacggt
    55021 ggcgtactta tcgtcactca gcgtgtgctt gatatacgga gcctcacgca attgcttgag
    55081 gtagtcgctc agacgcatcc cttctaccag tcgcagcggg aactgtgctt ctttaccgct
    55141 ttccaacaat ttcagcatct cgcgcacggt catctgcggt gtaaagcggt aagtcccggc
    55201 tttaaagtga gaaagatccg gttcgatacg cagcagccat tgaaaaaccc gtgggcgatt
    55261 gatgatctta tcagcataaa gctgttcacc gagcgccaga cgtccggtcc ctggcttcag
    55321 ggtaaatatc gtctcttctt tgataagcaa tttgctgtcg gcaagatggc gaaccttcca
    55381 gacgcccaca ccagcggcga tacccagtac caccagcaat aacaagataa tcaataacac
    55441 ttttttcatg actaattcgg gcgctcacaa agtggggcta aatattcata taacgttgct
    55501 gacgaaaagg tgacatcgcc acaggcacgt acgggcatca ctggcattaa cgcattacaa
    55561 ataaccatct catctgcctg caacgcctcc tccagcgagg cttgcacttc gacaagctga
    55621 taagaggatt gtgccagtaa acggatacag aattgtcgca taatgccgtt aacacctgcc
    55681 tgatccagtc gcggcgtata aactacattg cccttccgcc agaacaaatt agccgcacag
    55741 cattccgtaa cccacccttc gctgtcaagg accagcgcct catcagcgtt tgtctgctca
    55801 agatgagagc gaatcaatac ttgctcaagc cgattaagat gtttaatacc tgcaagatga
    55861 ggattgcgcc ccagccgcac cgggcttagc gccaacgtca tcccttcgtt acgcaaacgg
    55921 tcgtaatgtg caggataagc cgtaacggag agaatccgcg ttgctggtcc gctgttcaat
    55981 gtgctgtacc ctcgcccgcc actaccgcga ctgatcacga ctttcagcac accattttgc
    56041 tgttctgctg ccagcgtttt catctcttgt tcaagctgag gccagaagtc acaggaaatc
    56101 atcaaccgct gacaagcatc ctgtagtcgc aggatatgcg ccgataacaa actgacttta
    56161 ccgtcgataa ctctggcggt ggtaaaacaa ccatcaccaa actgcgttgc ccgatcgctt
    56221 actgccagcg attcctgctt ataaccgtta attaagaaca taccggctcc ttatcagaga
    56281 attgttagtg tggcagcatg ttcactacgg aacaagtcgg aataaaagct aagaaaaaag
    56341 gcccgcaagc ggacctttta tatgggtgga aaatgacaac ttagatcttt ttaaagatca
    56401 aagaaccatt agtgccaccg aagccgaagg agttacacag cgtgtattcc attccgctga
    56461 cctgacgtgc ttcatgcggt acgaaatcca gatcgcaacc ttcatccggg ttatccaggt
    56521 tgatggttgg cggaacagcc tgatcgcgca gcgccaggat ggagtagata gattctactg
    56581 cacccgccgc acctaacagg tgaccggtca tagatttcgt ggagcttacc aacacacggc
    56641 ttgcagcttc accgaagatg gttttcaccg cctgcgcttc agctttatcg ccagccggcg
    56701 tagaagtacc gtgcgcgtta acgtagccaa tctgactcgc ttcaatgcct gcatcacgca
    56761 gagcatttgc cattgccaga gctgcgcctg cgccattttc tggcggtgac gtcatatgat
    56821 aagcatcgct gctcatacca aagccgacga gttcagcgta aattttcgca ccgcgttttt
    56881 tcgcgtgttc gtactcttca agtaccagca taccggcacc atcgcccagt acgaagccat
    56941 cacgctcttt atcccacggg cggctcgccg cttgcgggtt atcattgcgg gtagataatg
    57001 cacgtgccgc gccaaaacca ccaacgccca gcggcgtact ggctttctct gcgccacctg
    57061 caaccatcac gtcagcatcg ccatacgcga taatacgcgc agcatggcca atgttgtgca
    57121 cgccggaagt acaggcggtc gcgatagaga tgcttgggcc acgcaggcca tacatgatag
    57181 tcagatgacc tgccaccatg ttcacaatcg ttgacggaac gaagaatggg ctgatcttac
    57241 gtggaccacc gttcatcaga gatgtgtggt tttcttcgat cagtccgagg ccgccaatcc
    57301 cggagccaat tgcggcacca atgcgggttg cgttctcttc cgttatttca aggccagaat
    57361 cctgcatggc ctgaacgcca gcgacaattc catattgaat gaaggcatcc atcttgcgct
    57421 gttctttgcg cgagataatg tcctcacagt taaaatcctt tactaagcca gcaaatttcg
    57481 ttgcataggc gctagtatcg aaatggtcga ttaggctgat gccactctga ccggcaagca
    57541 gagctttcca ggtagactct acggtattgc cgacaggaga caacatgccc agtccggtca
    57601 caactacacg acgcttagac acgtttgtcc tccaaggagg gaaaaatgat tctagtggga
    57661 caaaaagata aaactcaggc ggtcgaacga ccgcctggag atgttcactt acgcctggtg
    57721 gccgttgatg taatcaatgg cagcctgaac ggtggtgatt ttctcagctt cttcgtccgg
    57781 aatctcagta tcaaactctt cttccagagc cattaccagc tcaacggtgt caagagaatc
    57841 cgcgcccagg tcttcaacga aagaagcatt gttggtaact tcttcctgct taacgcccag
    57901 ctgttcgccg ataattttct taacgcgttc ttcgatagtg ctcatactct taaatttcct
    57961 atcaaaactc gctttcgcga tggttttcgt agtgtataaa atgttgaaaa atttgcaact
    58021 aaatcccggc aggtcttacc acgattttac gttattttga ggccttaccc cccaataacg
    58081 caaatcattt tgcactaatt attactcata accacgcaga ctgcgcgcaa catgagcatt
    58141 ttgtgcaaat cgcggtcaga ccatgtacat cccgccgttc acatgcaaag tttcacccgt
    58201 gatgtaagct gcttcgtcgg atgccaggaa tgcaaccgcg ttggcgattt cctgtgcgcc
    58261 gccgaggcga cccgcaggaa cctgcgccag gatacccgca cgctggtcat cgctcagcgc
    58321 acgtgtcatg tccgtttcaa taaagcccgg agcaacaacg tttacagtaa taccgcgtga
    58381 cgcaacttcg cgcgccagtg atttactgaa gccgatcaag cccgctttcg ccgcagcgta
    58441 gttggcctga ccgccatttc ccatggtacc aaccacagaa ccgatagtga taatacgacc
    58501 atgacgcttt ttcatcatag cgcgcattac cgcttttgac agacggaaaa cagatgaaag
    58561 gttagtttcg ataatatcgt tccactcttc atctttcatt cgcattaaca ggttatcacg
    58621 agtgataccg gcattattga ccaggatatc cacttcacca aattctgcgc gaattttttc
    58681 cagaacagat tcgatagatg ccgggtcggt cacattcaac atcagacctt tgccgttggc
    58741 acctaaataa tcactgatcg cctgagcgcc attttcactg gtcgcagtgc caataacttt
    58801 cgcgccacgg gctgcgagcg tttcagcaat tgcgcggcca attccgcggc ttgcaccggt
    58861 taccagtgcg atttttcctt caaaattcat gattttcctc ttttaaagct cgagcgccgc
    58921 tgccatcgct gaaggttcgt tcagcgccga ggcggtcagg gtgtcgacaa tgcgtttcgt
    58981 caggccagta agcaccttgc ccgggccgac ttcatagaga tgttctacgc cttgcgctgc
    59041 catgtactca acagacttcg tccactgaac cgggttatac aactgacgta ccagtgcgtc
    59101 acggatggca tcaccattgg tttcgcattt cacatcaacg ttattcacaa cagaaactgt
    59161 tggtgcgtta aaggtgattt tcgctaattc tactgccagt ttgtcggctg ctggtttcat
    59221 cagcgcacag tgagacggta cgctcactgg taacggcagc gcgcgttttg cgccagcagc
    59281 tttacaggca gcgccagcac gctcaaccgc ttctttatga ccggcaataa ccacctgtcc
    59341 cggagagtta aagtttaccg gagaaacgac ctgaccttct gcagcttctt cacacgcttt
    59401 cgcaataggc gcatcatcca gaccgatgat tgccgccata gcgcccgtgc cttccggtac
    59461 ggcttcttgc atgaacttac cgcgcatctc aaccagacgc accgcatcag cgaaatcaat
    59521 cacgccagcg caaaccagcg cggagtattc ccccaggctg tgaccggcca tcattgccgg
    59581 tgctttaccg ccctgctgct gccatacgcg atacagcgca acagatgcag tcaacagcgc
    59641 tggctgggtt tgccaggttt tattcagttc ttcagctgac ccctgctggg tcagcgccca
    59701 caggtcgtag cccagcgccg cagaagcttc agcaaacgtt tcttcgacaa ttggatagct
    59761 cgccgccata tcagccagca ttccaacggt ttgagaaccc tgtccaggga acacaaatgc
    59821 aaattgcgtc atgttttaat ccttatccta gaaacgaacc agcgcagagc cccaagtgaa
    59881 tccaccgcca aaggcttcaa gcagaaccaa ctgccccggc ttaatgcgcc cgtcgcgtac
    59941 agcttcatcc agcgcgcacg ggacagaggc cgcagaggta ttaccgtggc gatccagcgt
    60001 caccacgaca ttgtccatcg acataccgag ttttttcgcc gttgcactga taatacgcag
    60061 gttagcctga tgcggaacca gccagtccag ttgagaacgg tcaagattat tcgccgccag
    60121 cgtctcatca acgatgtgcg ccagttccgt taccgcaacc ttgaagactt cgttgcccgc
    60181 catcgtcaga tgaattgaat tctctggatt cacgcggtcg gcgtttggca gcgtcagcaa
    60241 ttcaccataa ctaccgtcgg catgcagatg ggtggaaatg attcccggct cttcagaggc
    60301 agccagcacc gcagcgcccg cgccatcgcc aaaaataata atagtcccac gatctgttgg
    60361 atcgcaggtg cgcgccagta catcggaacc gacgaccaga gcatacttca ccgccccaga
    60421 tttcacgtat tgatcggcta cgcttaatgc ataggtgaaa cctgcacagg ctgctgcaac
    60481 gtcaaatgcc gggcaacctt taatgtccaa catgctttga atctgacaag ctgcgctcgg
    60541 gaaagcgtgc gtagcagaag tcgttgccac aacgatcagg ccaatctggt ctttctcaat
    60601 gcccgccatc tcaattgcgc gtgtcgccgc ttcaaagccc atggttgaaa cggtttcgtt
    60661 tggcgcggca atgtggcgtt cgcggatacc ggtacgagtg acaatccact cgtcagaggt
    60721 gtccaccatt ttttccaaat cggcgtttgt ccgcacttgt tcgggcagat agctgccagt
    60781 accaataatc ttcgtataca tgtacgctca gtcacttttc ggttatatac cgtcacttgc
    60841 aaactgcgag ttcgctggca gcgtcctgct accgcagagt tccgcttttg ccaccgtcca
    60901 gcagctcaaa accagctggg tatacagatt ccaggcgagc ggcaattcgc tgaggaactt
    60961 gtcgctgcac cgcctgcact gcctgttcaa tcgcgaccgc aaaagctcgc tgattggctg
    61021 caccatgact ttttatcacc gtgccgcgca atcctaacag acaggcgccg ttatactggt
    61081 cggggttgag gtgactgaat cgcctcgtca ggctcttttg tagccaacgc tttaataaca
    61141 gtagccacca cgaccgtttt ttcccttcac cctgagattt cagcagagaa aggaacatcc
    61201 tgacaacacc ttccatcgtc tttaacgtga catttcctgt aaagccgtca caaaccagca
    61261 catctgtctt gccagttaac aactcattgg cttcaagata gccgatataa ttgatagaag
    61321 ggattgtttt aagcaccgct gaggcatccc gaatactgtc aagacccttt acttcttctt
    61381 caccaatatt gagcaacgcc acgcgaggat tgggaatttc caccacctct tcagccagaa
    61441 ctgagcccat aatggcaaat tgcaccaaca ttgtgctgtc acaatcgacg ttggccccta
    61501 aatcgaggac caccgttttg cccttttgct gatgtggtaa taccgtcacc agcgccggac
    61561 gctcaatccc ctccaggggc ttgagtaata attttgccag ccccatcagt gccccggtat
    61621 taccggcact gacacaggct tgcgctcgac cttctttcac cagctccagg gccatacgca
    61681 ttgaactccc acggctggcg cggatagctt gcgaaggccg ggcatcactg gcgataactg
    61741 actgcgcagg aataatctgc agacgcgaac gttgttcaaa gtcagcttta gcaagtaatg
    61801 gcgtgatggc gtcgggattg ccgactaaaa gaagagtgag ttgcgaatta gaattcagtg
    61861 cctgcaatgc tgcaggcact gtcacggaag ggccaaaatc ccctcccatg acatctaacg
    61921 ccagggttag acgtgtcaag gtatcgtcgc cgcccggttt ggtctttccc cagttgcctg
    61981 gggaaaatcc tcactaagct tcatcacgca gacgcgtgat tacttagcga tgaccttgcg
    62041 gccgcggtag taaccgtcgg cagtgatgtg gtgacgcagg tgtttttcac cagaagtttt
    62101 gtctacagac aggctggtga ctgcggtcag cgcgtcatgg gaacgacgca tgccacgttt
    62161 ggaacgggtt ggtttattct gttgtacggc catggacctt actcctcaat tacggtttat
    62221 ccccgatcca acggggagca ccaattactt acgctttaag ctggctaata cggcaaatgg
    62281 gtttggcttt tgcacttctt caggcagttc accaaagacc atgtccgctt cggacacttc
    62341 acagtgttca gaatcatgca ccggaactac cggcaaggcg aggatgattt catcttcaac
    62401 cattgcaagc agatcgattt caccgaattc gttaacctca atcggttcat acgcttccgg
    62461 cagtgcttca gcctgttcgt ctgaacgcac aggactaaaa caatacgttg tgtagacctg
    62521 atgagtaaac ggcttcccgc aacgctgaca ctcgagcgtt accgtcacct tcgcatcgcc
    62581 gtttaacact gcgagacgtt ggttatcgat agcgaacgac atggagcatt ccacatcact
    62641 gtccacactg actacggatt cggcgacgcg ctcaacctga tcaggggtat agataccctg
    62701 gtaatcaagg cgtttttgag ccgtacgaac cggatcgaga gtcaggggta attttacctt
    62761 ttgcataggg cgcgcatatt aactttgtaa cgtcatagag tcaaagaaaa aggcagcctg
    62821 tggttgcctt ttgccaataa ttcgcacaca ttgcgggtta ctgctttatt ttcgttcagt
    62881 gctgataacg aacatcaacc tgcaaaacga agcgcatagt ttaaaatggc tatcatcaat
    62941 acgctatatc tgatggaaaa aaatatgcct aaacttattt tagcctccac ctcgccatgg
    63001 cgtcgcgctc ttctggaaaa actgcaaatc tcttttgaat gtgcagcacc tgaggtcgac
    63061 gaaaccccac gcagcgacga atcaccgcga cagttggtgc ttcgactggc acaagaaaaa
    63121 tcgcaatctc tggcgtcacg ttatccggat catttaatta ttggttcaga ccaggtatgt
    63181 gttcttgatg gcgaaatcac cggtaaaccg ttaacggaag aaaatgcccg tctgcaatta
    63241 cgcaaagcca gcggcaatat cgtcaccttc tatactggac tggcgttgtt taattcggcg
    63301 aatgggcatc tacaaacaga agtggagcct tttgacgtcc atttccgtca cctaagcgag
    63361 gcggagattg ataattacgt gcgtaaggag catcccctgc actgcgcggg tagctttaag
    63421 agtgaaggat ttggcattac gctgtttgag cgtttagagg gacgtgaccc taacacgctg
    63481 gttggtttac cgctaatcgc gctgtgtcag atgttacgtc gggaagggaa aaacccgctg
    63541 atgggatagt tgtttgcgcc ggatgcggtt acgtcgcatc cggcaatcgg tgcaggcggc
    63601 ctgatgcggc gtaaacgcct tatcaggcca accgcccatt atcaggctta tatgcttgta
    63661 ttagcgcgcg ttacgcagct tttgcaaaca acgcttcaaa ccttcatcca tcggcgcttc
    63721 gatacgcatc acctcaccgg tccccggatg ggtaaacttc aacgctgcgg cgtgcaggaa
    63781 cagacgattt aatcccgtgc ctgcttcagt gagctgtctg tcaaattcac ggtcaccgta
    63841 gcgatcgtca aaggcaatcg gatgacccgc atactgtgta tgcacacgga tctgatgagt
    63901 acgtccggtt accgggctac aacgcaccag ggtggcaaat gcatagcgtt cttccacttt
    63961 aaagcgtgtt tccgacggtt tgccttcctg actgacacgc acgatacgtt cgccgctttg
    64021 cagaatattt ttcaataacg gcgcttgaac gctcttcaca tgcgactgcc actgaccgcg
    64081 caccagcgcc aggtaatctt tttgcattcc tttttcacgt aattgctcat gcagagaacg
    64141 caacgccgag cgttttttcg ctaccagcaa cacacctgaa gtgtcccggt cgagacgatg
    64201 aaccagttca aggaaccgcg cttccgggcg caacgcccgc aaaccttcga taacgccgaa
    64261 gcttaaacca ctgccgccat gtaccgccgt accggaaggt ttattcagca ccaggatatg
    64321 atcatcttca tataagatga cgtccgccag cgccgccacc ttttgcagat gtggcgaaac
    64381 cgcctcttct tcccgctcag caacgcgaac cggtgggata cgcacctcat cacccgcttc
    64441 gagtttatat tcaggcttaa tacgtttttt gttcacccgc acttcacctt tacgcaaaat
    64501 acggtaaatc atactttttg gtacgccttt caattgggta cgcaaaaagt tatcgatacg
    64561 ttgccccgct tcgtcagcgg tgatagcaac aatttttacg gatggagtct ctgttttcat
    64621 ggtgggcgat tctaattagc caacaggatt cgcgccactc atttttctat gcttattttt
    64681 agttttgcac cttattactt cactacgtga tcactttatt gatggttatt aaaccaatca
    64741 ccagcaagaa gtgaaaaaac tgtgagtaag cgggtgataa atggtaaaag tcatcttgct
    64801 ataacaaggc ttgcagtgga ataatgaggc cgtttccgtg tccatccttg ttaaaacaag
    64861 aaattttacg gaataaccca ttttgcccga ccgatcatcc acgcagcaat ggcgtaagac
    64921 gtattgatct ttcaggcagt tagcgggctg cgggttgcag tccttaccgg tagatggaaa
    64981 tatttctgga gagtaatacc cagtctgttt ctttgataat tgcgctgttt ttccgcatga
    65041 aaaacgggca accgacactc tgcgcctctt tgagctgacg ataaccgtga ggttggcgac
    65101 gcgactagac acgaggccat cggttcacac ccggaaaggc gttactttgc ccgcagctta
    65161 gtcgtcaatg taagaataat gagtaagtta cgatgaaaag aatgttaatc aacgcaactc
    65221 agcaggaaga gttgcgcgtt gcccttgtag atgggcagcg tctgtatgac ctggatatcg
    65281 aaagtccagg gcacgagcag aaaaaggcaa acatctacaa aggtaaaatc acccgcattg
    65341 aaccgagtct ggaagctgct tttgttgatt acggcgctga acgtcacggt ttcctcccac
    65401 taaaagaaat tgcccgcgaa tatttccctg ctaactacag tgctcatggt cgtcccaaca
    65461 ttaaagatgt gttgcgtgaa ggtcaggaag tcattgttca gatcgataaa gaagagcgcg
    65521 gcaacaaagg cgcggcatta accaccttta tcagtctggc gggtagctat ctggttctga
    65581 tgccaaataa cccgcgcgca ggtggcattt ctcgccgtat cgaaggcgat gaccgtaccg
    65641 aattaaaaga agcactggca agccttgaac tgccggaagg catggggctt atcgtgcgca
    65701 ccgctggcgt aggcaaatct gctgaggcgc tgcaatggga tttaagcttc cgcctgaaac
    65761 actgggaagc catcaaaaaa gccgctgaaa gccgcccggc tccgttcctg attcatcagg
    65821 agagcaacgt aatcgttcgc gccttccgcg attatttacg tcaggatatc ggcgaaatcc
    65881 ttatcgataa cccgaaagtc ctcgaactgg cacgtcagca tatcgctgca ttaggtcgcc
    65941 cggatttcag cagcaaaatc aaactgtaca ccggcgagat cccgctgttc agccactacc
    66001 agatcgagtc gcagatcgag tccgccttcc agcgtgaagt ccgtctgcca tccggtggtt
    66061 ccattgttat cgacagcacc gaagcgttaa cagccatcga catcaactcc gcacgcgcga
    66121 cccgcggcgg cgatatcgaa gaaactgcgt ttaacaccaa cctcgaagcc gccgatgaga
    66181 ttgctcgtca gctgcgcctg cgtgacctcg gcggcctgat tgttatcgac ttcatcgaca
    66241 tgacgccagt acgccaccag cgtgcggtag aaaaccgtct gcgtgaagcg gtgcgtcagg
    66301 accgtgcgcg tattcaaatc agccatattt ctcgctttgg cctgctggaa atgtcccgcc
    66361 agcgcctgag cccatcactg ggtgaatcca gccatcacgt ctgcccgcgc tgctccggta
    66421 ccggtaccgt gcgtgacaac gaatcgctgt cgctctctat tctgcgtttg atcgaagaag
    66481 aagcgctgaa agagaacacc caggaagttc acgccattgt tcctgtgcca atcgcttctt
    66541 atctgctgaa tgaaaaacgt tctgcggtga atgcgattga aacgcgtcag gacggtgttc
    66601 gctgcgtgat tgtgccaaac gatcagatgg aaaccccgca ctaccacgtg ttgcgcgtgc
    66661 gcaaagggga agaaacgccg accttaagct acatgctgcc gaagctgcat gaagaagcga
    66721 tggcgctgcc gtctgaagaa gagttcgctg aacgtaagcg tcctgaacaa cctgcactgg
    66781 caacctttgc catgccggat gtaccgccag cgccaacccc agctgaacct gccgcgactg
    66841 tcgtagcccc agcaccgaaa gctgcaacgg caacaccagc agctcctgca caacctgggc
    66901 tgttaagccg cttcttcggc gcactgaaag cgctgttcag cggtggtgaa gaagccaaac
    66961 cgaccgagca accaacaccg aaagcagaag cgaaaccgga acgtcaacag gatcgtcgca
    67021 aacctcgtca gagcaaccgc cgtgaccgta atgagcgccg cgacacccgt agtgaacgta
    67081 ctgaaggcag cgataatcgc gaagaaaacc gtcgtaatcg tcgccaggca cagcagcaaa
    67141 ctgccgagac gcgtgagagc cgtcagcagg ctgaggtaac ggaaaaagca cgtaccaccg
    67201 acgagcagca agtgccgcgt cgtgaacgta gccgccgccg taacgatgat aaacgtcagg
    67261 cgcaacaaga agcgaaggcg ctgaatgttg aagagcaatc tgttcaggaa accgaacagg
    67321 aagaacgtgt acgtccggtt cagccgcgtc gtaaacagcg tcagctcaat cagaaagtgc
    67381 gttacgagca aagcgtagcc gaagaagcgg tagtcgcacc ggtggttgaa gaaactgccg
    67441 ctgccgaacc aattgttcag gaagctccag ctccacgcac agaactggtg aaagtcccgc
    67501 tgccagtcgt agcgcaaact gcaccagaac agcaagaaga gaacaatgct gataaccgtg
    67561 acaacggtgg catgccgcgt cgttctcgcc gctcgcctcg tcacctgcgc gtgagtggtc
    67621 agcgtcgtcg tcgctatcgt gacgagcgtt atccaaccca gtcgccaatg ccgttgaccg
    67681 tagcgtgcgc gtctccggaa ctggcctctg gcaaagtctg gatccgctat ccaattgtac
    67741 gtccgcaaga tgtacaagtt gaagagcagc gcgaacagga agaagtacaa gtgcagccga
    67801 tggtgactga ggtccctgtc gccgccgctg tcgaacctgt tgttagcgcg ccagttgttg
    67861 aagaaatggc tgaagtcgta gaagcccccg taccggttgc cgaaccgcaa ccggaagtgg
    67921 ttgaaacgac gcatcctgaa gtgattgccg ccgcggtaac tgaacagccg caggtgatta
    67981 ccgagtctga tgttgccgta gcccaggaag tcgcagaaca cgcagaaccg atggttgaac
    68041 cgcaggaaga gacggcagat gcagatattg aagaagttgc cgaaactgct gaggttgtgg
    68101 ttgctgaacc tgaagttgtt gctcaacctg ccgcgccagt cgtcgctgaa gtcgcagcag
    68161 aagttgaaac ggtggccgcg gttgaaccag agatcaccgt tgagcataac cacgctaccg
    68221 cgccaatgac gcgcgctccg gcaccggaat atgttccgga ggcaccgcgt cacagtgact
    68281 ggcagcgccc tacttttgcc ttcgaaggta aaggtgccgc aggtggtcat acggcaacgc
    68341 atcatgcctc tgccgctcct gcgcgtccgc aacctgttga gtaataatta gctcaaagta
    68401 atcaagccct ggtaactgcc agggcttttt tatttcatgt ttgaatcaac atattaaaat
    68461 tcatcaaatg gcatccttgc taaccaacaa tgcaaaatag gcaaaaaaaa tgcccgcccc
    68521 ggcgatccca gagcgggcag acaaaacata cccagtttca tgatatgttt taaagcgagc
    68581 ttatttgttg agctggaaga gcgacaatcc ctgcatatcg gtaaatgctt tatacgatgc
    68641 ctgcaatgcc gtttgctgca tgatgtaaga tgaaatagtt gcattccagt ccacatcaac
    68701 cagatcgctc atctgctgcg tttgccctaa agcgcgatcg ctacctaagg aatccagcga
    68761 ctccagtttg ttcagctgcg tgcctaattc cgcgcgaacg gtaaggacat tattcagcga
    68821 gtttttcagt ccacggttgg ttttatccaa tgccgcagcg gcgatttctt tatccgcttc
    68881 gctatccgcg accggcgttt tcagggctgc gatggcgcta tccagcatgg caaaaagatt
    68941 ggtttcagaa gcgctaccgt ctggttccgc taccgcgtta ctggtaatac tgtcgaaaat
    69001 tttgtcgccc gtatgtccta tcaccatcga acgcgaagca tcgacctgtt gcttaatact
    69061 ttctgttccg ccaacataat caccgttagc ctcactaaac ggcgcagtct ctgttttata
    69121 tgtagcgccc attaccgtca gtggtgttcg ccagattcag caactggtca cgaagcccct
    69181 gaatatccgt agccagcgag gcccggtcat cgtcactcaa ggtgccattg ctggcgtaga
    69241 caattttttc ctgagcattc tggatagcag tggtgacctg gctaagtaca ctctcttcca
    69301 gtgacacttt ttgagtggcg aaagtacgtg ccagcgtgta ctggctgttt tgcgcctgtg
    69361 cctgggagag aacaacagct tgtgatgcag caatgggatc gtctgaaggg ttaacgactc
    69421 gcttacccgt cgacatctgc tcgccgtact tcatccattc tgcctgagaa ttggtgatgc
    69481 cgcgcatgtt ttgctggtac atcatctggg tactgaaacg cattttatct ccccttagcg
    69541 aatgttaatc agcgcatcaa aaatcgcgtt tgccgtctgc agaacctgcg cattcgccag
    69601 gtaatactgc tgaaaacgtt gcagatttcc gtactcctca tcgagattga caccggaaat
    69661 cgactgctgc tgattggaaa gctgcgtcac cacattacct tgcgtggtgc tactggtttt
    69721 caacgtcgcg gttttattac cgatatcact cactaacgag gcataagcgt cgttaaagga
    69781 tttcgcaccg cccaccgttt tactgttgct ttgcagatcc agcagggcct gaccgttgcg
    69841 gttgtcgcta tcacccgcat cttcttcgct cgccatcgcg attttcgctt cgtcggtgat
    69901 taatacatcc atgttgacga tggcgtcact tactggtttc agcgtgaagc tgtcgttaac
    69961 ggcaggcgtt cctgtaaacg tcaactccag accatcaaat gccactttac cgttggcatc
    70021 tggtgtcgcc gtaaaagtgg tattgctggc aaggcgggtg acctgccact gattattatc
    70081 gaacgagatt ttgtaatctg tcgccagtac cacggaggca tcagttaccg tggcaccgat
    70141 cgcaacgtca cctttgtttt tcgtgttttg cagaaccgcg ggcttaccga tagtaaagaa
    70201 atcttcacca gcatcgccgt tagcatcaaa tccggctttg tgttggctgt tgaaagcctc
    70261 ggcaaatgcc agcgccagtt gtccaagcgt attacgcgtc tggtccagat cctgagaacg
    70321 gaatgtcaga atgccgccca gcgacccggt attcagtaat ttctccggga tctcaatatt
    70381 gcctgccgtc ccatcaacgt aagcgacagt cgtacgagaa gggtcggcgc tggaaggaac
    70441 tgccgccagt tgccgcgccg tacttccctg aaccagtgag taaccattgg catcgtgatg
    70501 ttataagtac cgccatcctg aacgctgact tctacgccaa caatctggtt taattcgctc
    70561 accagttgat cgcgttgatc cagcagattg ttaggtgacg cccctgcccc cacgcctgtc
    70621 aggcgcgaga tttgatcgtt caggctggca atttgtttag cgtaattgtt gatctgatca
    70681 acgctggcac ctatcgcgat attgacctgt ttgtcctggt cgcgcagata ttgatcggtg
    70741 gttttaaact gattcaccaa cccttctgat ttcccaatca gcgcctggcg cgccgccggg
    70801 tcttccgcgt tactcaccag cgtttgcagg ctggtgaaga aatcctgcat ctgtgttgcc
    70861 agcgaagagg tactggtgga gagcatattg tcgattttcg acatctgctc atagcgggcg
    70921 gtcaggccgc tactttgcgt ctgcgcagca cgtaactggt tggtaataaa cgtatcatac
    70981 tcacgctgca caccagaaac gtagacgcca ttgccaaccc agccgccagc gcccaacgtg
    71041 ctattggcct gcgccataat agtggtttgg cgggtatatc cggcaacgtt atagctggag
    71101 atattattac ttgccgtatt taacgccgcc tgggccgcgt tcagtccgct catggcgtta
    71161 ttgatcaagc tggacatgga ggttccttat aagccttcaa ttacagagta ttatcggcag
    71221 cgacccgccg gacttgagtt attcagaaca gattatcaat gttcatgctg taggttttgc
    71281 tcaccttgtc gcttatcgat ttcatctgct ggatcatgtt ggtgagttta cgggcatagt
    71341 gaggatcggt agcatagccc gcgtcctgta ggacctgcgc cccctgttcc gcactcgcgg
    71401 cggtcgtcac ggcggcgtag cgcgggttac gtgttaacag cccaacgtaa tccgacaatg
    71461 cttccagata cgagctgtag acccgaaact tcgcttttac tttcttcgct tcgccgtttt
    71521 catattcagt cgtggtgatt tcagtgactt gccctttcca gttgccagag gctttgacac
    71581 caaacaggtt atagcttggc tcgccgtttt cgcggcggat ttgccgttgc ccccaaccag
    71641 attcaagcgc agcctgagcg aggatcaaat gatgtggcac accgctttgc tggcttgcca
    71701 gttgggcggg cagcgagagt tgcgcgagga atgctctact gtcacccggc agcgaatcat
    71761 cgtagttacg tggtacggcc ttttgcacca gctgcgaaag cgtctggttt tgataacgca
    71821 ccacggtttc gagcgggaat ttcatcggtg ctgctggcgt ggactcctct ggcaatggtt
    71881 gttctggcgt catctgttta accatcatct ctgcaagccc cagacctttg cccgccgtca
    71941 tctgttgggc aatttgctgg tcatacatac tggtatacag acgagtgtgc tcgctgctga
    72001 acaggccatc ttttggtaaa gcgtcgcgca tgcttttcaa catcatctgc acgaacatcc
    72061 cttccacctg acgggccacc ggacggatat ttgccgccgg atcttcgctc gctttcgcct
    72121 ttagttcgtt gagtgattgc gcatcccagg ccgcacttgc cagtagtttg ctgtcgctga
    72181 tcatcagatg atttccagtt ttgcccgcag acatcccgca ctttgcattg attgcaaaat
    72241 agacatcaga tccatcggcg tagcgcccag cgcattgagc gcacgcacca cgttattgag
    72301 gctggcgctg gaacgtacgc tttgcagcga accgccgctc tggcgtaaat cgatctgcgt
    72361 ttgtggagta acaacagtct gtccaccacc aaacggtgta tctggctggc tgacattggc
    72421 ctgacgatta actgttactg agagatttcc ctgcgctacc gcgcagctgt cgagggtcac
    72481 ttcgcgattc atcaccaccg aaccggtgcg cgagttaatc actactttag cgtcctgcgg
    72541 ggtgacatta acctgcatat tctggatatc ggcaaggaaa cggacctggg aactgttgcc
    72601 acttggtacg cgcacctgaa tagtccgcgc atctaacgcg gtggcgctgc catatccacg
    72661 cacgcggttg atggtgtcag cgatttgctg cgccatgctg aaatcttcgt cgttaagttg
    72721 caaattaagg gtattcccga cgccaaactg gctgggcaat tcacgttcaa taaccgcacc
    72781 attggtgatc cgtccaccgt tcagttggtt aacctgaaca ctgctaccgc ctgcggaggc
    72841 tcctgcgccg ccaaccagaa tattgccctg cgccagcgca tacacctgac tgtcaacgcc
    72901 cttaagcgga gtcatcaaca acgtacctcc acgcaagctt ttggcattac ccatggaaga
    72961 aaccaccaca tcgatggttt gcccctgacg tccaaacgga ggaagtgacg ctgtcaccat
    73021 taccgcagcg acgtttttta gctgcatatt ggtgcccgtc ggaacggtaa ttcccagctg
    73081 tgagagcatg ttattaagcg tttgtgtggt aaacggcgtc tgggttgtct ggtcaccagt
    73141 gccatccagc cccactacca agccatagcc aatcagtgag ttttgcctta ccccctgaac
    73201 actggtgaga tcgcgaatac gctcagcctg agccgccgtc gtaaccagta gaagaattaa
    73261 tgcagagaga aatttaatca ccacagcctc acttacattg gcgacaggtt aaggaagaaa
    73321 cgctgcaacc agcccatatt ttgcgcttcg ttaatgtagc cattgcctac gtattcaata
    73381 cgcgcatccg ccacctgagt agacggtacg gtattgctgc cgctgatagt gcgtggatta
    73441 accacgcccg agaagcgaat aaattcggta ccctgattaa tggcaatctg tttttcaccc
    73501 accacatgca ggttgccgtt gaccagtacc tggtcaaccg tcaccgtcaa cgtgccgcta
    73561 aaggtattgc tggcattggc cccgcccttt ccgttgaacg tgttaccacc ggaggcttcg
    73621 acatcggcac gagcgttacc aaacagcccc tgcaaatagc gcggcacagt atcaaagcca
    73681 aaattagttt taccgtcacg gctggcattc gcagaggagc ttttgctggc gctgacgttc
    73741 tcctgcaaca cgatggtcag cgtatcgcca atattgcgtg gtcgacgatc ttcaaacagc
    73801 ggttgatagc catagttaat cggctgagca gactggaaaa tagaaccgtt ggcgacgggc
    73861 gtcggaccgg gaaccggttg tgcactggtc gccccctgca ccagcggcgt ggagggtatc
    73921 caggcgcagc cggttagtga aagcaccaac aagctggaaa tggcataagt atgcgcagcg
    73981 tttttttgca tggctatcat cttcaaaaat cagtaaaccg gtgaacctgc caccggttat
    74041 gccttagagt tgcgtcagtt tttgcagcat ctgatcggtg gtggacaccg ctttactgtt
    74101 gatttcgtaa gcgcgttgca cctgaatcat attgaccagt tcttccgcca cgttgacgtt
    74161 agacgtttca acataccctt gatacagcag tcccgcgccg ttcaggcccg gcgtgctttc
    74221 gttcggtgca ccggaggatt gcgtttcggt gtagaggttt tcgccaatgc tctccagtcc
    74281 ggtatcattc ataaaggtgg tgagattgag ctgcccaacc tgaaccggag ctgcctggcc
    74341 ttgttgggtt acgctgacca cgccatcacg accgatggtg atacttaacg cattcgccgg
    74401 aatggtgatc gctggctgca cctgaaaacc accagccgtc accagctgcc cgttctgatc
    74461 cacctggaaa gagccgtcac ggatataggc tgatgagcca tctggcaaca ttacctggaa
    74521 aaagccctgc cctttaatcg cgacatcttt gctgttattg gtctgcgaca gattgccctg
    74581 gctgtgtaag cgttcagtgg cgaccgggcg tacccccgtg ccgatttgta atccggaggg
    74641 taaggtggtt tgttcggaag actgtgcccc cggctggcga atggtttgat aaagcagatc
    74701 ttcaaacacc gcgcgctgac gcttaaaacc gttagtactg acgtttgcca ggttgttggc
    74761 aatgacgtcc atattggttt gctgggcgtc aaggcccgtt ttggcgatcc ataatgaact
    74821 gatcatataa tgtcctgtag tggattgctg acaagcagag cagaagcacg cgacagtgtg
    74881 aatttcccct caccctaacc ctctccccaa aggggcgagg gaatagtccg cgcgctttta
    74941 ttgcaatgtc atcaatcgtt caattaactc atcgacagca gttggttggc acggcctgcg
    75001 ttatcatcga cgctgctgat caccttcatc tgcatttcaa aacgccgcgc gctggcaatc
    75061 atgtcgctca ttgccgcaac ggcattgacg ttactgcctt ccagaacccc cgacatcaca
    75121 cgcaaggttg gatctgcctg cagtaccggc cctcgcgtgg cctgggtttc tgcgcttaaa
    75181 cgaaaaatgc cgtcgtcacc gcgctgcact tcgctgcccg tggctttcac cagtttcaga
    75241 cgccctactg gcgcaactgt atttgccgga tcgcccggat tgagcgccga gattgtgcca
    75301 tcggcagcaa tcgtgatttc cgccccttcc ggcacagcaa ttggccccgc ctcgcctatc
    75361 accggatgcc cctgaattgt cagttgcccg gtgggatcaa cctgaatgct gccattacgc
    75421 gtataccctt cgctgccgtc agcggtctgc acggccagcc atcctgctgc aacgcaacgt
    75481 ccagcgggcg agaggtgtaa tccatcttgc cgggcgtcat gtctgcaccc ggtgttgacg
    75541 ccgttaccag cgtgcgcgtg ggcagtgaaa gcccttccac cggcactgcg cgcaaggcgt
    75601 ttaactgcgc gcgaaaaccg ggcgtagagg cattggccag attactggcg gttaccgcct
    75661 gttgattcag cgtctggctg gctgctccca tcgcagtata aattgcgtga tccattgagc
    75721 tatcccgtca gcgattagcg taagttaacc cgcgtgttga ggatctggtc ctgggttttg
    75781 atggtctggg cgttagactt atagttacgc tgggcaacga tcatattgac cagttcttta
    75841 ctgagatcga cgttggacgc ttccagcgca ccgttggtca gagtgccaaa gtttcccgtc
    75901 ccggctgtcc ccaacagcgc cacgccagaa gattgcgtcg cagaccagac gttgtcgcct
    75961 tcggatgcca gaccttcgtt gttggcaaag ttcgccagta caatctgccc cagcagttgg
    76021 gtttgttcgt tggaattgtt gccgacaacc gtaccgtcat cattgatttg ataactcacc
    76081 agatcgcccg gtttgtagcc attctgggtg gttgccacaa tgttgttagc gccggttgcg
    76141 atattattcg ccatcgcacc atccactaat gtgccattag cattaaattc cagtgttgtc
    76201 gctgtcttcg caatgctgtt tggatcactg ctatcctggg tgtagacctg ccagttatta
    76261 tccccggtct tcacaaaata gacgctcatg tcatgagcat taccctgact gtcgaaaaca
    76321 gtcaccgaac cttttttgtt atagctatcc gcattgctgg cgctgaatgg cgtaacagta
    76381 ggaagcggat cactggaatt caggttgatc tgcatcgacg ccgtggtggt agttttcgct
    76441 gccatcaggg tattcgggat cgaaatattg gtcggattcg ccccttgctg aatagtcggc
    76501 ggcgtaccgg ttaccgggta acccgtcagc tgtaaacctt gcgtattcaa caggttacgg
    76561 ttttcatcca gcttaaattg tccgttacgg ctgtagaaca ccgaaccgtt gctgtctacc
    76621 agacggaaaa aaccgttctg gctgatagca acgtccagac ctcgcccggt gttggtggtc
    76681 gtgccatcgg taaagtcctg agtgataccg gcaactttta cccccagtcc cactttcgaa
    76741 ccggcaaaca tatcggcaaa agaggccgtg cctgatttaa agccgtaggt ggcggagttg
    76801 gcgatattgt tgccaataac atcgaggttg gtggcggcag cgtttaatcc gctaaccgct
    76861 tgagaaaagg ccatgactga ctcctgaaat gtgaaggctt aaattatctg ccgtacttcg
    76921 tcgagggtgg tggtgccgta agtgccgaga tccagcgtat taccgctgtt gccgcggatc
    76981 acaccctgca ccagagcaaa ctgcagcggc tgggcaacca gttgtgtacc accgttactg
    77041 gcgctaatcg ctacattgta agaaccgttc ggcgcagtgc tgccatcagt caacgaaccg
    77101 tcccaggtga aactgtgaac tccggcggtc agttcaccaa tatcaatggt gcgcacaacc
    77161 gcgccatttt tatcggtgat ggtggccgta actttgtctg ccgcctgttg cagctcaaca
    77221 ccaaacggcg tggtcgtggt cacagcccct tcttcactgc cggttcccgc aagaacagtt
    77281 gtgccgggga tcatcacgcc gtgaccgatc aggttactgg cctgtaacga ctggctgtta
    77341 tcaatctgtc cggaaataga tccgagcgtg gtattgagtt tttcaatccc gctgaccgtg
    77401 ctgatttgtg ccaattgcga cgtcagctcg ttgttttcca ttggattggt cgggtcctgg
    77461 tttttcagct gcgccaccag caaagtcaga aaactgcttt gtaaatctgc ggcgttgctg
    77521 cccgtgagcg aactactgct ggtggtactg acgccggtat ttgtcggatc ggtggtggtt
    77581 accgcaatgg acatagcttt ctcctttatt gaccgagcgt aagggttttc agcatcatgc
    77641 ttttcaccgt gttgagcact tcaacattgg cctgatagct gcgtgacgcc gacatggtgt
    77701 taaccatctc cccgacgaca tcaacgttcg gcatttttat gtagcccttt gcatctgcca
    77761 gcggattacc tggttcataa accagtttgt ccggggcctg actttcttaa catcggcaac
    77821 ctttacgccg cctgtcgcag cacctggtgc agcgttaacc tggaatacca cctgttttgc
    77881 ccgatatggc tgtccatcag gaccggtcac gctatcagca tttgccagat tactggccgc
    77941 cacgttcagg cgctgggact gggcagttaa cgccgacccg gcgatatcaa aaatattcag
    78001 cagtgccatc cgttaatttc cgctctgtaa aacgttcatc atgcctttga tttgcccgct
    78061 caacgcgcta aggctcatct ggtattgcag gctgttatcg gcaaactggg tgcgttcgcg
    78121 atccatatcg acggtattac cgtcaagcga aggctggtcc ggaatacggt attgcagttc
    78181 tgcggtagga ggcgtcagcg cctgcgccgg gatgtgctgc gttgaggtca tcgtcagtgc
    78241 aaccacactg gttgcatccc gtccacgttg catgactttt ttaagttcac tggcaaaatc
    78301 gatatcgcgc gcctgataac caggggtatc ggcattggcg atgtttgctg ccagcacttc
    78361 ctgacgctgg gcgcgcagat tgagcgcctc ttgttgaaaa cgtaaggcgg cgtcgagctt
    78421 atcgagcata tctcctccgc aggtatcaaa attctgccat cagcttaaat gcctttacgc
    78481 gcgcgttatc ggcggaataa acgcaaaatg ggtcgctatt tatgccgttg atggtcattg
    78541 cggacaggta caattcacgt tgtagaaatg gctgggggcg aaaatgctga caataaaacg
    78601 tagcgtggcg atcatcgcga tactgttcag tccgttaagt acggcgagca atctcacatc
    78661 gcaattgcac aactttttta gcgcccaact cgcgggggta agtgatgagg ttcgtgtttc
    78721 tattcgtaca gcgcccaatc tactaccgcc atgcgagcag ccattgcttt cgatgagcaa
    78781 taattcccgc ctgtggggca atgtgaatgt gttggcacgc tgcggtaacg acaaacgata
    78841 tttacaggtt aatgtgcagg ccacaggaaa ttatgtggtt gccgcgatgc ctattgtgcg
    78901 gggaggaaag ctggaagctg gcaatgtcaa actgaaacgt ggacgcctgg ataccctgcc
    78961 accgcgtacg gtgctggata tcaatcaact tgttgatgcc gttagcctgc gcgatctatc
    79021 acccgatcaa cctatccagt taacccagtt tcgccaggca tggcgggtaa aagcgggaca
    79081 acgcgtcaat gtgatcgcca gcggtgatgg gtttagcgcc aacgcagaag gtcaggcgct
    79141 gaacaatgca gccgtcgcac agaatgcgcg ggtgcgcatg gtatcggaac aggtagtcag
    79201 cggcgttgtt gatgcagatg ggaatattct tataaacctg taagctgtta aagattaccc
    79261 gtcccttgcc gataaataag caacacatga taaaagcgcc ctcaatgagg aataaaccat
    79321 gagtattgat cgcacttcgc ctctgaagcc tgtaagcacc gttcaaccgc gcgaaaccac
    79381 cgacgcgccg gtaacgaaca gccgggcggc aaaaacaacc gcctccacca gcaccagtgt
    79441 gacgttaagc gacgcgcaag caaaactgat gcaacccggc agcagtgata tcaatcttga
    79501 acgtgtcgaa gcgttaaaac tggcgattcg taacggtgaa ctaaaaatgg acaccggcaa
    79561 aattgccgat gcgctgatca acgaagcgca gcaagacttg cagagtaact gatcgtatga
    79621 cacgtcttgc agagatcctc gaccagatgt ccgctgtgct taacgatctc aaaacggtaa
    79681 tggatcaaga gcagcaacat ctctctatgg ggcagatcaa cggcagccag ttgcaatgga
    79741 ttacagaaca aaaaagctca ctgctggcga cgctggatta cctcgaacag ttacgcaaga
    79801 aagaacccaa tacagcaaat agcgttgata ttagtcaacg ctggcaggaa attactgtga
    79861 aaacgcagca actacgccaa atgaatcaac ataacggctg gttactggaa ggacagattg
    79921 agcgcaatca acaggcgctg gaaatgttga aaccgcatca ggaaccgacg ctatatgggg
    79981 cgaacggtca gacctcaaca ccccatcgcg gcagtaaaaa gatttcgatc tgaatattat
    80041 ctccggcctg cactgcaggc cggaatgcat tgttacaccg tccggcgggc aaattcttta
    80101 actttgaagc ccagtaccgc cagtgcagcg aagtacgcgg caatccccgc cagcacgacc
    80161 gccattaaac gcagtaagcg ccagggcatg gtacccaatg accactccgg catgatatgt
    80221 aacataccta aaagcacgcc agacatcacc agtaccgcca ccaccagacg caacagaaac
    80281 gccatccagc cgggttgcgg agtaaagatt ttctgcttac gcaactgcca gtaaagcagc
    80341 gaagcattca gacacgccgc cagaccaata gaaagtgaca gcccggcatg tttcaacgga
    80401 ccaataaacg ccaggttcat caattgcgtt aaaatcagcg taacgatggc aattttcact
    80461 ggcgttttaa tgtcctggcg ggaataaaag ccaggagcca acactttcac tacaatcagg
    80521 ccaatcaaac ccaccgagta ggcaattaac gcccgctggg tcatcagcgc atcaaacgcg
    80581 gtaaatttac cgtactggaa cagcgaaacg gttaacggac ctgaaagaat gcccaacgca
    80641 accgcactcg gcagtgccaa caggaaacaa agacgcaacc cccagtccat caaacggttg
    80701 tattcatcat gattgccact ggcaaaactt ttcgacagcg acggcagcaa aatggtgcca
    80761 agcgccacgc ccagcacacc ggagggaaac tccattaagc ggtcggcgta atacatccaa
    80821 gacaccgaac cggaagcaag aaacgaggca aaaatggtgt tgatgattaa ggagatctgg
    80881 ctcacagaga cgccaaggat cgccggtccc atctgtttca ccacgcgcat tgctccagca
    80941 tcgtggaagt taatgcgcgg caggaccagc atgccgatct tcttcaggtg cggtagctga
    81001 tacaccagct gcagaatgcc gccgaccgta acagcccacg ccagcgccag caccggtggg
    81061 ttaaagtacg gtgcggcaaa cagcgcgaaa ccaatcatgc tgatgttaag cagtgttggc
    81121 gcaaacgccg gaatcgagaa gcggttccac gtattcagaa tcgctcccac cagcgacgcc
    81181 agggagatca gcaagatata gggaaaggta atctttagta gctggctggt cagggcaaat
    81241 ttgtcagctg tgtcagcgaa gcctggcgcg gtcaccatga tcacccacgg tgcggcgagc
    81301 atgccagcga ccgtcacaac cgccagcgca agtgtcagca ggccagaaac ataagagaca
    81361 aagacccgcg tggcgtcttc accctgctta cttttatatt ccgccagaat cggtacaaat
    81421 gcctgggaaa atgccccttc ggcaaagata cggcgtaaca agttaggaag tttaaaagcg
    81481 acgaaaaagg cgtcggttgc catccctgcg ccaaagattc tggcgacaat tgcgtctcgt
    81541 gcgaagccaa gcacacgcga aaacatggtc atcgagctga cggcggccag cgattttaat
    81601 aaattcatcg gtgttctaat ccggacccac gggcaaaacg cctgcaaggc aggcgctgaa
    81661 agaggcgaat agtctaccta gattatggtg aattgctacc gccagatgtt acagggttta
    81721 ttcactcatc gcatcgcgcc agatcttgtc aacgatacgt tgcgccagca ctgcctattc
    81781 gccggcggtt tgcggaactg tctgatttta cacgcattca atgaagtgcc gcgcacagcc
    81841 gacaaatcca cgttgctcaa gagtgctctg ccaaccagga atcggtttat tcactacacc
    81901 ctgcccgcgc tcctcacgcc attcgcgcat atccgtaatg tcgatgagcg caccgtcagt
    81961 cacggcctgc acggtttcac gctgacttcc ggcacggcga tgcatacaag tggtgatctg
    82021 caaaggaccg gccgaaaaat ggtgctcggc aaacagcatt tcgccagcgt cgttagtcag
    82081 tagcgtaccg ccatccagag aggctttgcc gcccgacaac cacagcgcgg tatccaccac
    82141 atgcagataa tcatccagca acgtgaaata aagatcgtgt ggcccgacgc tattgctacg
    82201 atgtttatcc attcttagcg aggctgcggt ggcgagttgc gtttttaact caccgtagag
    82261 tggtgcgaaa cgacggttaa aaccgaccat caacgtcagt tttttccgcg ccgccagttc
    82321 caccagcctt tcagcatcgc acagattttc tgccagcggt ttatcgacac agacatgtac
    82381 ccccgcattg agtaacgtac tgaccacgtc aaagtggctg gcggtgctgg agtgcacaaa
    82441 aaccgcatca caactggcgg caaggctgga taacgaatcg gcataaggaa tgcgccagct
    82501 gtcacaaatt ggcagggctt tggcgcgcgt aggcgaccag gctccttgta acgtccagtc
    82561 agacgctgcc gccagcaccg gtaaccacgc tttttgcgca atgccgccta atcccactac
    82621 gccgatacgt aattttttca ctttaatctc ccagatgggc cagcaacgaa tcaagacgtt
    82681 gtttcagttc tgccacttcg atttccaggg cttcgacgcg ggtctgtaaa tcaccgtcaa
    82741 ccatgttcga catagccgtt accgccggct gatcttcaac ctcaccactg aaaagatgca
    82801 tgtagcggct ttcgcgttta cccggttcgc gggccagacg caccacaaaa ggaccatctt
    82861 cgcgattcgc cagttgttcc agcgtcgact ccacttccgc catatcgctg aattcataca
    82921 ttcgcgcggc gcggctgcgc agttcacccg gcgtctgggc accacgcaat aacaacgtgg
    82981 tgattaacgc cacttctgcg gcgctcagtt tcagatcgcc aaattctgaa ttacaaaaac
    83041 gttgctcata tttggtgacc cgattaccaa aaccgctcac tgtgcgtaga taatgacgtt
    83101 tgaccagatt atccagctgt tcctgcactt cggattcact cagattcatg accggttcac
    83161 ggttcgtttt ctgattacag gccgtgacta caccattgac tgagagcggg tattgctccg
    83221 gcgtggtcac ctgcttttcc agcaagcaac caattacgcg agcttccagg gcagtaagtt
    83281 gatatttcat gagattctcc ttagcggccg ggcgtccagt ctggggtagt taatgccgtc
    83341 agtacgtgat cgcgccattg tccatcaatc aacagatagt ctttcgcata gccttctttt
    83401 tcaaaaccca gtcgcgccag taaatcaccg ctgcgtttat tgtgcggcat ataattagcc
    83461 ataatgcgat gaatatgttg ggtgcgctgc atataacgaa tggctgcggt cagggcttca
    83521 aacatgagtc ctttgccctg ccatttttgc ccaatcgaat aaccgagata gcaggcatga
    83581 aaagagccgc gaacaacatt ggaaaaattg gcaacgccaa taatctcttt ttcgtccggg
    83641 tcgaataagc caaagtagaa agctgaacct tgtttatgaa attcgttaat catccctagc
    83701 ctggcctgcc agcctgatgg ataacagtgg ctttcgtcgc gcactggctc ccagggcttg
    83761 aggaaatggc gattctctgc gtaataatcc gccagacgcc aggcatcacg atcatgcacc
    83821 agacgcacga ccagtcggtc tgtggttaag cgcacttttg gcacgttact gcgatagcca
    83881 aacattctat acctactcct tcccgtaacg tctttaatac gctgtaagta ttactatacc
    83941 tgcgcaacca gaccctgtga aaagtccaaa atgccatttt ctgcattatt cacatatttg
    84001 ataaaaactc tctatcaaag cgacgttttg ataaaaaaat actgtccccg aagtgatggg
    84061 aaaagtctta gcggcaatgc agaatagaaa gtgctcatct tttatttccc gggaggggaa
    84121 atgtcccgcg tatcgcaggc gaggaacctg ggtaaatatt tcctgctcat cgataatatg
    84181 ctggtcgtgc tggggttctt tgttgtcttc ccgctgatct ctatccgctt cgttgatcaa
    84241 atgggctggg ccgccgtcat ggtcggtatt gctctcggtc tacgccagtt tattcagcaa
    84301 ggtctgggta ttttcggcgg cgcaattgcc gaccgctttg gtgccaaacc gatgattgtt
    84361 accggtatgc tgatgcgcgc cgccggattc gccacaatgg gtatcgccca cgaaccgtgg
    84421 ctattgtggt tttcatgcct gctctcggga ctcggcggca cgttgtttga tccgccgcgt
    84481 tcggcgctgg tggtgaaact aatccgtcca caacaacgtg gtcgtttttt ctcgctgttg
    84541 atgatgcagg acagtgccag tgcggtcatt ggcgcattgt tggggagctg gctgttgcaa
    84601 tacgactttc gcctggtctg cgccacaggg gccgttttgt ttgtgctatg tgcggcgttc
    84661 aatgcgtggt tgttaccggc atggaaactc tccaccgtac gcacgcccgt tcgcgaaggc
    84721 atgacccgcg tgatgcgtga caagcgtttt gtcacctatg tcctgacgct ggcgggttac
    84781 tacatgctgg ctgtacaagt gatgctgatg ctgccaatta tggtcaacga cgtggctggc
    84841 gcgccctctg ccgttaaatg gatgtatgcc attgaagcgt gtctgtcgtt aacgttgctc
    84901 taccctatcg cccgctggag tgaaaagcat tttcgtctgg aacaccggtt gatggctggg
    84961 ctgttgataa tgtcattaag catgatgccg gtgggcatgg tcagcggcct acaacaactt
    85021 ttcaccctga tttgtctgtt ttatatcggg tcgatcattg ccgagcctgc gcgtgaaacc
    85081 ttaagtgctt cgctggcgga cgcaagagct cgcggcagct atatggggtt tagccgtctg
    85141 ggtctggcga ttggcggcgc tattggttat atcggtggtg gctggctgtt tgacctgggc
    85201 aaatcggcgc accagccaga gcttccgtgg atgatgctgg gaattattgg catcttcact
    85261 ttccttgcgc tgggttggca gtttagtcag aaacgcgccg cgcgtcgttt gctggaacgc
    85321 gacgcctgat ttgatactcc cccctgactc ctccatactg aaagtgaact gacagccaaa
    85381 tggaggagtc acgtgaagct atacatttac gatcactgcc cttactgcct caaagcccgc
    85441 atgattttcg gcctgaaaaa tatccccgtc gaattacatg ttctgctcaa cgacgacgca
    85501 gaaacaccca cccggatggt cggtcaaaaa caggttccca ttctgcaaaa agatgacagc
    85561 cgctatatgc cagaaagcat ggatatcgtt cactatgtcg ataaactcga cggcaaaccg
    85621 ttactgaccg gcaaacgttc ccctgccatt gaagagtggc tgcgcaaggt caatggctac
    85681 gtcaacaaac tgctgttgcc gcgttttgcc aaatcggcat ttgatgagtt ttctactccc
    85741 gccgcgcgca aatatttcgt cgacaagaaa gaggccagcg cgggtaattt tgccgacctg
    85801 ctggcccact ctgacggtct gattaagaat atcagcgatg atttgcgtgc gctggacaaa
    85861 ctgatcgtca aaccgaacgc cgtgaatggc gaactttcgg aagatgatat tcagctattt
    85921 ccgctactgc gtaatctgac gctggtagcc ggaatcaact ggccaagccg cgttgctaat
    85981 taccgcgata atatggcgaa acagacacaa atcaatttgt tatcatcaat ggcgatttaa
    86041 tcctgacccg ccgcggcagc ctgtattcgc gggttgccgc cgttttcagt tttttctgct
    86101 ggcgagttta tcgctggtga cacatgctgc gttgtgtcag gatattgacg cagatttcat
    86161 gtcgaggact accatgaaca agtttttatt tgccgctgca ttgatcgtca gtggcctgct
    86221 cgttggctgt aatcaactca cccaatacac catcaccgaa caagaaatta accagtcgct
    86281 tgcgaaacat aataatttct caaaagatat cggtttaccc ggcgtggctg acgcccatat
    86341 tgttctgaca aacttgacca gccaaattgg tcgcgaagag ccgaataagg ttaccctaac
    86401 cggagacgcc aatctggaca tgaactccct gttcggtagt cagaaagcga ccatgaaact
    86461 gaagctgaaa gcgctgcccg tgtttgataa agaaaaaggt gcgatcttcc tgaaagagat
    86521 ggaagtggtc gatgcgacgg tacaaccgga aaaaatgcaa acggtgatgc aaacgttgct
    86581 tccctatttg aaccaggcat tacgcaatta ctttaaccag caacctgctt acgtcctgcg
    86641 cgaagatggc agtcagggcg aagcaatggc gaaaaaactg gcgaaaggca ttgaagtgaa
    86701 gccaggcgaa attgtcattc cgtttacgga ttaatgacga gggcgcattc gcgcccttta
    86761 tttttcgtgc aaaggaaaac gtttccgctt atcctttgtg tccggcaaaa acatcccttc
    86821 agccggagca tagagattaa tgactgcacc atcccaggta ttaaagatcc gccgcccaga
    86881 cgactggcac cttcacctcc gcgatggcga catgttaaaa actgtcgtgc cgtataccag
    86941 cgaaatttat ggacgggcta tcgtaatgcc caatctggct ccgcccgtga ccacagttga
    87001 ggctgccgtg gcgtatcgcc aacgtattct tgacgccgta cctgccgggc acgatttcac
    87061 cccattgatg acctgttatt taacagattc gctggatcct aatgagctgg agcgcggatt
    87121 taatgaagcc gtgttcaccg ctgcaaaact ttacccggca aacgcaacca ctaactccag
    87181 ccacggcgtg acgtcaattg acgcaatcat gccggtactt gagcgcatgg aaaaaatcgg
    87241 tatgccgcta ctggtgcatg gtgaagtgac acatgcagat atcgacattt ttgatcgtga
    87301 agcgcgcttt atagaaagcg tgatggaacc tctgcgccag cgcctgactg cgctgaaagt
    87361 cgtttttgag cacatcacca ccaaagatgc tgccgactat gtccgtgacg gaaatgaacg
    87421 gctggctgcc accatcactc cgcagcatct gatgtttaac cgcaaccata tgctggttgg
    87481 tggcgtgcgt ccgcacctgt attgtttacc catcctcaaa cgcaatattc accaacaggc
    87541 attgcgtgaa ctggtcgcca gcggttttaa tcgagtattc ctcggtacgg attctgcgcc
    87601 acatgcacgt catcgcaaag agagcagttg cggctgcgcg ggctgcttca acgccccaac
    87661 cgcgctgggc agttacgcta ccgtctttga agagatgaat gctctgcagc actttgaagc
    87721 attctgttct gtaaacggcc cgcagttcta tggcttgccg gtcaacgaca cattcatcga
    87781 actggtacgt gaagagcaac aggttgctga aagcatcgca ctgactgatg acaccctggt
    87841 gccattcctc gccggggaaa cggtacgctg gtccgttaaa caataaaaat cgtagcttcc
    87901 tgttgtcatt aggttatttt acctgtataa ataaccagta tattcaacag ggggctatta
    87961 tgcgaattga agtcaccata gcgaaaactt ctccattgcc agctggggct attgacgccc
    88021 tggctggcga actttcccgc cgtattcagt atgcgtttcc tgataatgaa ggccacgtat
    88081 cggtacgtta tgccgcagcg aataatttat cggttattgg cgcaacaaaa gaagataaac
    88141 agcgcattag cgaaattctc caggaaacgt gggaaagcgc cgatgactgg tttgtcagcg
    88201 aataatatgc agtgattttt gtttttgccg gatcgccccg gcttttttat tttacttacg
    88261 aattttgagt acaaaatttc tacacttcct caaaataatt gcacaatatg ctaaaaaaat
    88321 atgaatgaca tgctgtttta ttgttcatat aaatcttaaa agcagaaatt tatctgttcc
    88381 cccctttaaa aattcggtga atacccttac ttattggtat actgaagttg cttcagataa
    88441 aaacgcattg aacctcgaat aacgttgtct agtaacacga attagggggc catgatggaa
    88501 aaaaataatg aagtcattca gactcatccg ctcgtagggt gggacatcag caccgttgat
    88561 agctatgatg cgctgatgtt gcgtttgcac taccagaccc caaataagtc cgagcaggaa
    88621 gggactgaag ttggtcagac gctctggtta accactgatg ttgccagaca atttatttcg
    88681 atattagaag caggaatcgc caaaattgaa tccggtgatt tcccggtaaa cgagtatcgg
    88741 cgtcattaac atgacatgac catccaccca aaaggcacct caccggtgcc tttaccattt
    88801 ctgggttgta tatctgcatt cagttaatta ctctgcttaa accatgagag cagagagaaa
    88861 aaaatgaaat acgatctcat cattattggc agcggttccg taggcgctgc cgccgggtat
    88921 tatgcaaccc gcgccggttt aaacgtgcta atgaccgacg cccatatgcc accgcatcaa
    88981 cacggcagcc accacggcga tacgcgatta attcgccatg cttatggtga aggcgaaaag
    89041 tatgtcccgt tggtcctccg cgcgcaaacg ctgtgggatg aactttcccg ccacaacgaa
    89101 gatgatccca tttttgtacg ctctggtgtc attaacctcg gcccggctga ctccgcattt
    89161 ctcgccaacg tcgcccacag cgccgaacag tggcaactca acgttgaaaa gctcgatgcg
    89221 caagggatta tggcccgctg gccagaaata cgcgtcccgg acaactacat cggcttattt
    89281 gagactgatt ccggtttttt gcgcagcgaa ctggcgatta aaacctggat ccaactggcg
    89341 aaggaagcgg gctgtgcgca actgttcaac tgcccggtca ccgcaattcg tcatgacgat
    89401 gatggcgtaa ctattgaaac ggttgacggt gagtatcagg cgaaaaaagc gattgtctgc
    89461 gcgggaacat gggtaaaaga cctgctcccg gagctgcctg tccagcccgt acgtaaagta
    89521 tttgcctggt atcaggccga tggccgctat agcgtgaaga ataaattccc ggcgtttacc
    89581 ggtgaactgc ccaatggcga tcaatattat ggttttccgg cagaaaacga cgcgttgaag
    89641 attggcaaac ataacggagg ccaggttatc cattcagcgg atgaacgtgt tccgtttgcg
    89701 gaagtggcca gtgatgggtc agaagccttc ccgttcttgc gcaatgtatt gccgggtatc
    89761 ggttgctgcc tgtacggcgc tgcctgcacc tatgataatt cgcctgacga agattttatt
    89821 atcgataccc tacccggcca cgataataca ctgctcatta ccggcctgag tgggcacggt
    89881 tttaaatttg cgtcagtttt aggggaaata gctgccgatt ttgcgcaaga ccaaaaaagc
    89941 gattttgatt tgacgccatt caggctttcc cgcttccaat aatcatcatt acggcctcca
    90001 gaatgaggcc gtttttacag gagtatttaa tgcgtcggct gttgcactat ctcatcaata
    90061 atattcgcga gcatctgatg ctttatcttt ttctctgggg attgttggca attatggatc
    90121 tgatctatgt gttttatttt tagttttccg cactgacata aactgacaaa tatttaatct
    90181 ccctttcatt tagcgtgaaa aattgaatga gattattcct gcttctacag cactcaccag
    90241 acatctctga tgctgaaccc actaaactta tgaagattct ttcattatta attcaaaatt
    90301 attgaaataa aaataaatat cattcaattt gttatttttg tgttgcaatt cattctccga
    90361 cacgccattt tctttctctc gcaaattcgt attggatgca cttatgtcat tcacaaatac
    90421 ccctgaacgc tatggtgtta tatctgcggc cttccattgg ctaagcgcaa ttattgtcta
    90481 cggcatgttt gctttaggtc tgtggatggt cacgctcagt tattacgatg gctggtatca
    90541 caaagcaccc gaactgcata aaagcatcgg tattttgtta atgatggggc tggttattcg
    90601 cgttctgtgg cgtgtcatat ctccaccgcc gggtccgctg ccgagttatt cgcccatgac
    90661 tcgtcttgcc gcaaaagctg gacatctcgc attgtgccta ttgctgttcg ccatcggcat
    90721 cagcggctat ctgatctcaa ctgccgatgg taaaccgatc agcgtttttg gctggtttga
    90781 cgtccccgcg acccttgccg acgctggcgc acaggcagac tttgccggtg ccctgcattt
    90841 ttggctcgcg tggagcgtcg tagtactgtc tgttatgcac ggatttatgg ccctgaagca
    90901 tcatttcatc gataaagacg acactctgaa gcgcatgctg ggaaagtcgt catctgacta
    90961 tggagtataa aaaatgaaaa aaagcctgct tggtttaacc ttcgcgtccc tgatgttctc
    91021 tgccggttca gcggttgccg ccgattacaa aattgacaaa gaaggtcagc acgcctttgt
    91081 taacttccgc atacagcatc ttggctatag ctggttatat ggcaccttta aagatttcga
    91141 cggtactttt acctttgatg aaaaaaatcc ggctgccgat aaagtgaatg tgacaattaa
    91201 caccaccagc gtcgatacta accacgccga acgcgataaa catcttcgca gtgcggattt
    91261 tctcaatacc acaaaatatc cacaggcaac attcacctcc accagcgtga agaaagacgg
    91321 tgacgaactg gatattaccg gcgatctgac gctgaatggc gtaaccaaac ccgtcacgct
    91381 ggaagcgaaa ttaattggtc agggcgacga cccatggggc ggtaaacgtg caggctttga
    91441 ggccgaaggc aaaattaagc tcaaagactt caatatcaag acagatttag gtccagcttc
    91501 tcaggaagtg gatctgatta tttcagtgga aggcgtacag cagaagtaag agtgaaatgt
    91561 tgccggatgg cacgcatccg gcaatgatat ttattctgtt ggatcaggaa tacccagtgt
    91621 tgtattcaga cgtccacgag acttattaaa gatcttattg ccattttcac gtcctgcccg
    91681 acggcgtcgc tgttcttctg gcggtaacgc gctttcttcg cagcaaatct cactacaaca
    91741 acctttgtat ttttccgcgc atactggaca ctgaataaac agcaggtggc agccatcatt
    91801 tttacagttg gtatggctgt cgcacggcgc gccgcactgg tggcaatgcg cgataatctc
    91861 atccgatata cgttcgccca tccgctcgtc aaaaacaaaa tttttgccaa taaaacgcac
    91921 cggcaagccc tgctcgcgcg ccttacgggc gtattcaata attccgccct cgatatgcca
    91981 gactttgttg aatccgttat gtttcatcca ggcactggcc ttttcacaac gaatgccgcc
    92041 ggtgcagtac atgacgattt ttttatcttt atgtgcctgc atcatctcga ctgcttttgg
    92101 cagctgctca cggaaggtat ctgccggaat ttcgagcgcg ttttcaaagt gccccacttc
    92161 atactcatag tggttacgca tgtcgataaa tagtgcatcg ggatcgtcaa gcatggcgtt
    92221 cacttccgcc gcttgcagat actcaccaac attgctggca tcaaagtgag gatcgtcaat
    92281 accgtcggca acaatgcgat cgcgtacctt catgcgcagt acccagaagg atttcccgtc
    92341 atcatccaac gcgatattca ggcgtaaacc ctctaaagcc gggtcgaagg catagagctg
    92401 cgcgcgaaat gtttcaacat tgctcgcagg tacgctgatt tgcgcgttaa tgccctcatg
    92461 cgccagatac actcgcccaa aaacattcag cgcggtaaac agctgatata aagcgtcacg
    92521 ggtcgcctta ggatcggcga tgtggaaata cttgtaaaac gaaatggtgg ttcgctctca
    92581 gccaacattt tggcttttaa cgcgtcgttg gaaatgcggt tgtgtaacac tggcatggtg
    92641 tacggttcct gcgagatggg aaagtaaaaa tccgcggcat gatatagcaa ttatcgataa
    92701 ttaacatcca cacattttac gctacatttg cgcattaaaa attatttgtt atttacaagc
    92761 gcggcaattt cgcccagtct tcagccacaa attttggttg cgggcgaaaa aatgcgacaa
    92821 tacatacaat tgcccgtata ggttgaaaaa caggattgat atgacgaatc tacccaagtt
    92881 ctccaccgca ctgcttcatc cgcgttattg gttaacctgg ttgggtattg gcgtactttg
    92941 gttggtcgtg caattgccct acccgattat ctaccgcctc ggttgtggat taggaaaact
    93001 ggcgttacgt tttatgaaac gacgcgcaaa aattgtgcat cgcaacctgg aactgtgctt
    93061 cccggaaatg agcgaacaag aacgccgtaa aatggtggtg aagaatttcg aatccgttgg
    93121 catgggcctg atggaaaccg gtatggcgtg gttctggccg gaccgccgaa tcgcccgctg
    93181 gacggaagtg atcggcatgg aacacattcg tgacgtgcag gcgcaaaaac gcggcatcct
    93241 gttagttggc atccattttc tgacactgga gctgggcgcg cggcagtttg gtatgcagga
    93301 accggggatt ggcgtttacc gcccgaacga taatccactg attgactggc tacaaacctg
    93361 gggccgtttg cgctcaaata aatcgatgct cgaccgcaaa gatttaaaag gcatgattaa
    93421 agccctgaaa aaaggcgaag tggtctggta cgcaccagat catgattacg gcccgcgctc
    93481 aagcgttttc gtcccgttat ttgccgttga gcaggctgcg accacgaccg gaacctggat
    93541 gctggcacgg atgtccggcg catgtctggt gcccttcgtt ccacgccgta agccagatgg
    93601 caaagggtat caattgatta tgctgccacc agagtgttct ccgccactgg atgatgccga
    93661 aactaccgcc gcgtggatga acaaagtggt cgaaaaatgc atcatgatgg caccagagca
    93721 gtatatgtgg ttacaccgtc gctttaaaac acgcccggaa ggcgttcctt cacgctatta
    93781 aatctcccat gccggatgct tcagaatggc atccggcatt accacagcaa atccccctga
    93841 tttagcgata aaagctctct ggattgcgcc ccctggaagt cgggcgcata attagtgtgt
    93901 ttatcttttc ttcttatgtt caccgcgcct ggcgcaccaa cagcggattg ctatgtcacc
    93961 ctgtgaaaat gacaccccta taaactggaa acgaaacctg atcgtcgcct ggctaggctg
    94021 ttttcttacc ggggccgcct tcagtctggt aatgcccttc ttacccctct acgttgagca
    94081 gcttggtgtt accggccact ccgccctgaa tatgtggtcc ggtattgtct tcagtataat
    94141 gggttcctaa acacatcatt ttaggtattg aatatgagac gggcaatcag ctacatcaga
    94201 ttttcaagtg aacgacaact gaaaggggac tctgtacgcc gacaatctaa gctggttact
    94261 gactggcttg ataagaaccc tgagttttac ttggattcat ccttgagctt taaagatttg
    94321 gggaaaagtg cttttagtgg taagcacctg aaaggtggtc taggtgactt tttaaccgct
    94381 atcgagaaag ggcttgttaa agctggtgat acgctactaa tcgagagttt agaccgtctg
    94441 tcacgtcagg atattgatat tgcttctgag ctattacgcc gtatcttaag ggctggtgta
    94501 gatgtagtca cactgagcga tggagaacat tacacacgag aaagtttaaa agacccgtta
    94561 gccctgatta aaagcattct gataatgcag cgggcgcatg aggaaagttt gagaaagtct
    94621 gagcgtgtcc aagctgcatg gaaccgtaaa aaagaactca tctccgaagg tatcaaggta
    94681 tctaggcgtt gtcctgcatg gttaaggctg aatgatgacc gtcgaacatt cacaatcatt
    94741 cctgataaag tggaagttgt taaacgagca tttgacctga gattacaggg tttaagcttt
    94801 tgggctatca ccagaacttt aaacgatgaa ggtcacttgt cattgaatca atacacaccc
    94861 aaacaaaagg ggtggtctga tactgctgta aagaaactgc tcagaaatcg tgctgtgatt
    94921 ggatgtttca cacctgctgg acgtgaagaa gttcagggtt actaccccgc aataatcagt
    94981 gagtcactat tctatcgagt acagcaactg aacacagggc aatatggtag ggcttctgta
    95041 tcaagtaatc cgctatcagt caacttgttc aggg
//
LOCUS       DAJTRC010000003        75508 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.003, whole genome shotgun sequence.
ACCESSION   DAJTRC010000003 DAJTRC010000000
VERSION     DAJTRC010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 75508)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 75508)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..75508
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.003"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(425..631)
                     /locus_tag="OO357_000225"
     CDS             complement(425..631)
                     /locus_tag="OO357_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbfA family protein"
                     /protein_id="HCR5885307.1"
                     /translation="MELYREYPAWLIFLRRTYAVAAGVLALPFMLFWKDRARFYSYLH
                     RVWSKTSDKPVWMDQAEKATGDFY"
     gene            944..1033
                     /gene="kdpF"
                     /locus_tag="OO357_000226"
     CDS             944..1033
                     /gene="kdpF"
                     /locus_tag="OO357_000226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HCR5885308.1"
                     /translation="MSAGVIAGVLLVFLLLGYLVYALINAEAF"
     gene            1033..2706
                     /gene="kdpA"
                     /locus_tag="OO357_000227"
     CDS             1033..2706
                     /gene="kdpA"
                     /locus_tag="OO357_000227"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HCR5885309.1"
                     /translation="MAAQGFLLIATFLLVLMVLARPLGSGLARLINDIPLPGTAGVER
                     ILFRLPGVSDHEMNWKQYLCAILGLNMLGLAVLFFMLLGQHYLPLNPQQLPGLSWDLA
                     LNTAVSFVTNTNWQSYSGETTLSYFSQMAGLTVQNFLSAASGIAVIFAFIRAFTRQSM
                     STLGNAWVDLLRITLWVLVPVALLIALFFIQQGAQQNFLPYQAVNTVEGAQQLLPMGP
                     VASQEAIKMLGTNGGGFFNANSSHPFENPTALTNFVQMLAIFLIPTALCFAFGEVTGD
                     RRQGRMLLWAMSVIFVICVGVVMWAEVQGNPHLLALGADSSINMEGKESRFGVLVSSL
                     FAVVTTAASCGAVIAMHDSFTALGGMVPMWLMQIGEVVFGGVGSGLYGMMLFVLLAVF
                     IAGLMIGRTPEYLGKKIDVREMKLTALAILVTPTLVLMGAALAMMTDAGRSAMLNPSP
                     HGFSEVLYAVSSAANNNGSAFAGLSANSPFWNCLLAFCMFVGRFGVIIPVMAIAGSLV
                     SKKSQPASSGTLPTHGPLFVGLLIGTVLLVGALTFIPALALGPVAEYLS"
     gene            2729..4776
                     /gene="kdpB"
                     /locus_tag="OO357_000228"
                     /pseudo
     CDS             2729..4776
                     /gene="kdpB"
                     /locus_tag="OO357_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308752.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
     gene            4785..5357
                     /gene="kdpC"
                     /locus_tag="OO357_000229"
     CDS             4785..5357
                     /gene="kdpC"
                     /locus_tag="OO357_000229"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit C"
                     /protein_id="HCR5885310.1"
                     /translation="MSGLRPALSTFLFLLLITGGVYPLLTTALGQWWYPWQANGSLIR
                     EGDTVRGSALIGQNFTGNGYFHGRPSATAEMPYNPQASGGSNLAVSNPELDKQIAARV
                     AALRAANPDASTNVPVELVTASASGLDNNITPQAAAWQIPRIAKARNLSVEQLTQLIA
                     KYSQQPLVKYIGQPVVNIVELNLALDKLDE"
     gene            5350..8030
                     /gene="kdpD"
                     /locus_tag="OO357_000230"
                     /pseudo
     CDS             5350..8030
                     /gene="kdpD"
                     /locus_tag="OO357_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415223.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     KdpD"
     gene            8027..8712
                     /gene="kdpE"
                     /locus_tag="OO357_000231"
                     /pseudo
     CDS             8027..8712
                     /gene="kdpE"
                     /locus_tag="OO357_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415222.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator KdpE"
     gene            8987..9091
                     /locus_tag="OO357_000232"
     CDS             8987..9091
                     /locus_tag="OO357_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001312672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2618 domain-containing protein"
                     /protein_id="HCR5885311.1"
                     /translation="MENNNRLMPHIRRTTHIMKFAHRNSFDFHFFNAR"
     gene            9402..11599
                     /gene="speF"
                     /locus_tag="OO357_000233"
                     /pseudo
     CDS             9402..11599
                     /gene="speF"
                     /locus_tag="OO357_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308748.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase SpeF"
     gene            11596..12915
                     /gene="potE"
                     /locus_tag="OO357_000234"
     CDS             11596..12915
                     /gene="potE"
                     /locus_tag="OO357_000234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine-ornithine antiporter"
                     /protein_id="HCR5885312.1"
                     /translation="MSQAKSNKMGVVQLTILTMVNMMGSGIIMLPTKLAEVGTISIIS
                     WLVTAVGSMALAWAFAKCGMFSRKSGGMGGYAEYAFGKSGNFIANYTYGVSLLIANVA
                     IAISAVGYGTELLGASLSPVQIGLATIGVLWICTVANFGGARITGQISSITVWGGIIP
                     VVGLCIIGWFWFSPTLYVDSWNPHHAPFFSAVGSSIAMTLWAFLGLESACANTDVVEN
                     PERNVPIAVLGGTLGAAVIYIVSTNVIAGIVPNMELANSTAPFGLAFAQMFTPEVGKV
                     IMALMVMSCCGSLLGWQFTIAQVFKSSSDEGYFPKIFSRVTKVDAPVQGMLTIVIIQS
                     GLALMTISPSLNSQFNVLVNLAVVTNIIPYILSMAALVIIQKVANVPPSKAKVANFVA
                     FVGAMYSFYALYSSGEEAMLYGSIVTFLGWTLYGLVSPRFELKNKHG"
     gene            complement(12971..14611)
                     /gene="pgm"
                     /locus_tag="OO357_000235"
     CDS             complement(12971..14611)
                     /gene="pgm"
                     /locus_tag="OO357_000235"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HCR5885313.1"
                     /translation="MAIHNRAGQPAQQSDLINVAQLTAQYYVLKPEAGNAEHAVKFGT
                     SGHRGSAARHSFNEPHILAIAQAIAEERAKNGITGPCYVGKDTHALSEPAFISVLEVL
                     AANGVDVIVQENNGFTPTPAVSNAILVHNKKGGPLADGIVITPSHNPPEDGGIKYNPP
                     NGGPADTNVTKVVEDKANALMADGLKGVKRISLDEAMASGYVKEQDLVQPFVEGLADI
                     VDMAAIQKAGLTLGVDPLGGSGIEYWKRIGEYYNLNLTIVNDQVDQTFRFMHLDKDGA
                     IRMDCSSECAMAGLLALRDKFDLAFANDPDYDRHGIVTPAGLMNPNHYLAVAINYLFQ
                     HRPQWGKDVAVGKTLVSSAMIDRVVNDLGRKLVEVPVGFKWFVDGLFDGSFGFGGEES
                     AGASFLRFDGTPWSTDKDGIIMCLLAAEITAVTGKNPQEHYNELAKRFGAPSYNRLQA
                     AATSAQKAALSKLSPEMVSASTLAGDPITARLTAAPGNGASIGGLKVMTDNGWFAARP
                     SGTEDAYKIYCESFLGEEHRKQIEKEAVEIVSEVLKNA"
     gene            complement(14637..15182)
                     /gene="seqA"
                     /locus_tag="OO357_000236"
     CDS             complement(14637..15182)
                     /gene="seqA"
                     /locus_tag="OO357_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation negative regulator SeqA"
                     /protein_id="HCR5885314.1"
                     /translation="MKTIEVDDELYSYIASHTKHIGESASDILRRMLKFSAASQPAAP
                     VTKEVRVASPAIVEAKPVKTIKDKVRAMRELLLSDEYAEQKRAVNRFMLLLSTLYSLD
                     AQAFAEATESLHGRTRVYFAADEQTLLKNGNQTKPKHVPGTPYWVITNTNTGRKCSMI
                     EHIMQSMQFPAELIEKVCGTI"
     gene            15367..16131
                     /gene="ybfF"
                     /locus_tag="OO357_000237"
     CDS             15367..16131
                     /gene="ybfF"
                     /locus_tag="OO357_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5885315.1"
                     /translation="MKLNIRAQTAQNQHNNSPIVLVHGLFGSLDNLGVLARDLVNDHN
                     IIQVDMRNHGLSPREPVMNYPAMAQDLVDTLDALQIDKATFIGHSMGGKAVMALTALA
                     PDRIDKLVAIDIAPVDYHVRRHDEIFAAINAVSESDAQTRQQAAAIMRQHLNEEGVIQ
                     FLLKSFVDGEWRFNVPVLWDQYPHIVGWEKIPAWDHPALFIPGGNSPYVSEQYRDDLL
                     AQFPQARAHVIAGAGHWVHAEKPDAVLRAIRRYLND"
     gene            16271..16564
                     /gene="ybfE"
                     /locus_tag="OO357_000238"
     CDS             16271..16564
                     /gene="ybfE"
                     /locus_tag="OO357_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LexA regulated protein"
                     /protein_id="HCR5885316.1"
                     /translation="MAKEQTDRTTLDLFAHERRPGRPKTNPLSRDEQLRINKRNQLKR
                     DKVRGLKRVELKLNAEAVEALNELAESRNMSRSELIEEMLMQQLAALRSQGIV"
     gene            16704..17234
                     /gene="fldA"
                     /locus_tag="OO357_000239"
     CDS             16704..17234
                     /gene="fldA"
                     /locus_tag="OO357_000239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldA"
                     /protein_id="HCR5885317.1"
                     /translation="MAITGIFFGSDTGNTENIAKMIQKQLGKDVADVHDIAKSSKEDL
                     EAYDILLLGIPTWYYGEAQCDWDDFFPTLEEIDFNGKLVALFGCGDQEDYAEYFCDAL
                     GTIRDIIEPRGATIVGHWPTAGYHFEASKGLADDDHFVGLAIDEDRQPELTAERVEKW
                     VKQISEELHLDEILNA"
     gene            17444..17530
                     /gene="uof"
                     /locus_tag="OO357_000240"
     CDS             17444..17530
                     /gene="uof"
                     /locus_tag="OO357_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001491546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fur leader peptide"
                     /protein_id="HCR5885318.1"
                     /translation="MIRIISRANSVTSSNEVNRLVTGQIPHD"
     gene            17523..17969
                     /gene="fur"
                     /locus_tag="OO357_000241"
     CDS             17523..17969
                     /gene="fur"
                     /locus_tag="OO357_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric iron uptake transcriptional regulator"
                     /protein_id="HCR5885319.1"
                     /translation="MTDNNTALKKAGLKVTLPRLKILEVLQEPDNHHVSAEDLYKRLI
                     DMGEEIGLATVYRVLNQFDDAGIVTRHNFEGGKSVFELTQQHHHDHLICLDCGKVIEF
                     SDDSIEARQREIAAKHGIRLTNHSLYLYGHCAEGDCREDEHAHEGK"
     gene            complement(18053..18379)
                     /locus_tag="OO357_000242"
     CDS             complement(18053..18379)
                     /locus_tag="OO357_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885320.1"
                     /translation="MKKLILIAMMASGLVACAQSTAPQEDSRLKEAYSACINTAQGSP
                     EKIEACQSVLNVLKKEKQHQQFADQESVRVLDYQQCLRATQTGNDQAVKADCDKVWQE
                     IRSNNK"
     gene            complement(18416..19822)
                     /gene="chiP"
                     /locus_tag="OO357_000243"
     CDS             complement(18416..19822)
                     /gene="chiP"
                     /locus_tag="OO357_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitoporin"
                     /protein_id="HCR5885321.1"
                     /translation="MRTFSGKRSTLALAIAGVTAMSGFMAMPEARAEGFIDDSTLTGG
                     IYYWQRERDRKDVTDGDKYKTNLSHSTWNANLDFQSGYAADMFGLDIAAFTAIEMAEN
                     GDSSHPNEIAFSKSNKAYDEDWSGDKSGISLYKAAAKFKYGPVWARAGYIQPTGQTLL
                     APHWSFMPGTYQGAEAGANFDYGDAGALSFSYMWTNEYKAPWHLEMDEFYQNDKTTKV
                     DYLHSIGAKYDFKNNFVLEAAFGQAEGYIDQYFAKASYKFDIAGSPLTTSYQFYGTRD
                     KVDDRSVNDLYDGTAWLQALTFGYRAADVVDLRLEGTWIKADSQQGYFLQRMTPTYAS
                     SNGRLDIWWDNRSDFNANGEKAVFFSAMYDLKNWNLPGFAIGASYVYAWDAKPATWQS
                     NPDAYYDKNRTIEESAYSLDAVYTIQDGRAKGTMFKLHFTEYDNHSDIPSWGGGYGNI
                     FQDERDVKFMVIAPFTIF"
     gene            complement(20401..22065)
                     /gene="glnS"
                     /locus_tag="OO357_000244"
     CDS             complement(20401..22065)
                     /gene="glnS"
                     /locus_tag="OO357_000244"
                     /EC_number="6.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--tRNA ligase"
                     /protein_id="HCR5885322.1"
                     /translation="MSEAEARPTNFIRQIIDEDLASGKHITVHTRFPPEPNGYLHIGH
                     AKSICLNFGIAQDYKGQCNLRFDDTNPVKEDIEYVDSIKNDVEWLGFHWSGNVRYSSD
                     YFDQLHAYAIELINKGLAYVDELTPEQIREYRGTLTQPGKNSPYRDRSVEENLALFEK
                     MRAGGFEEGKACLRAKIDMASPFIVMRDPVLYRIKFAEHHQTGNKWCIYPMYDFTHCI
                     SDALEGITHSLCTLEFQDNRRLYDWVLDNITIPVHPRQYEFSRLNLEYTVMSKRKLNL
                     LVTDKHVEGWDDPRMPTISGLRRRGYTAASIREFCKRIGVTKQDNTIEMASLESCIRE
                     DLNENAPRAMAVIDPVKLVIENYQGEGEMVTMPNHPNKPEMGSRQVPFSGEIWIDRAD
                     FREEANKQYKRLVLGKEVRLRNAYVIKAERVEKDAEGNITTIFCTYDADTLSKDPADG
                     RKVKGVIHWVSAAHALPVEIRLYDRLFSVPNPGAADDFLSVINPESLVIKQGFAEPSL
                     KDAVAGKAFQFEREGYFCLDSRHSTAEKPVFNRTVGLRDTWAKVGE"
     gene            complement(22268..24214)
                     /gene="nagE"
                     /locus_tag="OO357_000245"
     CDS             complement(22268..24214)
                     /gene="nagE"
                     /locus_tag="OO357_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetyl glucosamine transporter subunit
                     IIABC"
                     /protein_id="HCR5885323.1"
                     /translation="MNILGFFQRLGRALQLPIAVLPVAALLLRFGQPDLLNVAFIAQA
                     GGAIFDNLALIFAIGVASSWSKDSAGAAALAGAVGYFVLTKAMVTINPEINMGVLAGI
                     ITGLVGGAAYNRWSDIKLPDFLSFFGGKRFVPIATGFFCLVLAAIFGYVWPPVQHAIH
                     AGGEWIVSAGALGSGIFGFINRLLIPTGLHQVLNTIAWFQIGEFTNAAGTVFHGDINR
                     FYAGDGTAGMFMSGFFPIMMFGLPGAALAMYFAAPKERRPMVGGMLLSVAVTAFLTGV
                     TEPLEFLFMFLAPLLYLLHALLTGISLFVATLLGIHAGFSFSAGAIDYALMYNLPAAS
                     QNVWMLLVMGVIFFAIYFVVFSLVIRMFNLKTPGREDKEDEIVTEEANSNTEEGLTQL
                     ATNYIAAVGGTDNLKAIDACITRLRLTVADSARVNDTMCKRLGASGVVKLNKQTIQVI
                     VGAKAESIGDAMKKVVARGPVAAASAEATPATAAPVAKPQAVPNAVSIAELVSPITGD
                     VVALDQVPDEAFASKAVGDGVAVKPTDKIVVSPAAGTIVKIFNTNHAFCLETEKGAEI
                     VVHMGIDTVALEGKGFKRLVEEGAQVSAGQPILEMDLDYLNANARSMISPVVCSNIDD
                     FSGLLIKAQGHVVAGQTPLYEIKK"
     gene            24547..25347
                     /gene="nagB"
                     /locus_tag="OO357_000246"
     CDS             24547..25347
                     /gene="nagB"
                     /locus_tag="OO357_000246"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008805656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HCR5885324.1"
                     /translation="MRLIPLTTAEQVGKWAARHIVNRINAFKPTADRPFVLGLPTGGT
                     PMTTYKALVEMHKAGQVSFKHVVTFNMDEYVGLPKEHPESYYSFMHRNFFDHVDIPAE
                     NINLLNGNAPDIDAECRQYEEKIRSYGKIHLFMGGVGNDGHIAFNEPASSLASRTRIK
                     TLTHDTRVANSRFFDNDVNQVPKYALTVGVGTLLDAEEVMIMVLGSQKALALQAAVEG
                     CVNHMWTISCLQLHPKAIMVCDEPSTMELKVKTLRYFNELEAENIKGL"
     gene            25407..26555
                     /gene="nagA"
                     /locus_tag="OO357_000247"
     CDS             25407..26555
                     /gene="nagA"
                     /locus_tag="OO357_000247"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HCR5885325.1"
                     /translation="MYALTQGRIFTGHEFLDDHAVVIADGLIKSVCPVAELPPEIEQR
                     SLNGAILSPGFIDVQLNGCGGVQFNDTAEAVSVETLEIMQKANEKSGCTNYLPTLITT
                     SDELMKQGVRVMREYLAKHPNQALGLHLEGPWLNLVKKGTHNPNFVRKPDAALVDFLC
                     ENADVITKVTLAPEMVPAEVISKLANAGIVVSAGHSNATLKEAKAGFRAGITFATHLY
                     NAMPYITGREPGLAGAILDEADIYCGIIADGLHVDYANIRNAKRLKGDKLCLVTDATA
                     PAGANIEQFIFAGKTIYYRNGLCVDENGTLSGSSLTMIEGVRNLVEHCGIALDEVLRM
                     ATLYPARAIDVEKRLGTLAAGKVANLTAFTPDFKITKTIVNGNEVVTQ"
     gene            26564..27784
                     /gene="nagC"
                     /locus_tag="OO357_000248"
                     /gene_synonym="nagR"
     CDS             26564..27784
                     /gene="nagC"
                     /locus_tag="OO357_000248"
                     /gene_synonym="nagR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NagC"
                     /protein_id="HCR5885326.1"
                     /translation="MTPGGQAQIGNVDLVKQLNSAAVYRLIDQYGPISRIQIAEQSQL
                     APASVTKITRQLIERGLIKEVDQQASTGGRRAISIVTETRNFHAIGVRLGRHDATITL
                     FDLSSKVLAEEHYPLPERTQQTLEHALLNAIAQFIDSYQRKLRELIAISVILPGLVDP
                     DSGKIHYMPHIQVENWGLVEALEERFKVTCFVGHDIRSLALAEHYFGASQDCEDSILV
                     RVHRGTGAGIISNGRIFIGRNGNVGEIGHIQVEPLGERCHCGNFGCLETIAANAAIEQ
                     RVLNLLKQGYQSRVPLDDCTIKTICKAANKGDSLASEVIEYVGRHLGKTIAIAINLFN
                     PQKIVIAGEITEADKVLLPAIESCINTQALKAFRTNLPVVRSELDHRSAIGAFALVKR
                     AMLNGILLQHLLEN"
     gene            27832..28584
                     /gene="nagD"
                     /locus_tag="OO357_000249"
                     /pseudo
     CDS             27832..28584
                     /gene="nagD"
                     /locus_tag="OO357_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide monophosphatase NagD"
     gene            28981..30645
                     /gene="asnB"
                     /locus_tag="OO357_000250"
     CDS             28981..30645
                     /gene="asnB"
                     /locus_tag="OO357_000250"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001724523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase B"
                     /protein_id="HCR5885327.1"
                     /translation="MCSIFGVFDIKTDAVELRKKALELSRLMRHRGPDWSGIYASDNA
                     ILAHERLSIVDVNAGAQPLYNQQKNHVLAVNGEIYNHQALRAEYGDRYQFQTGSDCEV
                     ILALYQEKGPEFLDDLQGMFAFALYDSEKDAYLIGRDHLGIIPLYMGYDEHGQLYVAS
                     EMKSLVPVCRTIKEFPAGSYLWSQDGEIRSYYHRDWFDFDAVKDNVTDKNELRQALED
                     SVKSHLMSDVPYGVLLSGGLDSSIISAITKKYAARRVEDQERSEAWWPQLHSFAVGLP
                     GSPDLKAAQEVANHLGTVHHEIHFTVQEGLDAIRDVIYHIETYDVTTIRASTPMYLMS
                     RKIKAMGIKMVLSGEGSDEVFGGYLYFHKAPNVKELHEETVRKLLALHMYDCARANKA
                     MSAWGVEARVPFLDKKFLDVAMRINPQDKMCGNGKMEKHILRECFESYLPASVAWRQK
                     EQFSDGVGYSWIDTLKEVAAQQVSDQQLETARFRFPYNTPTSKEAYLYREIFEELFPL
                     PSAAECVPGGPSVACSSAKAIEWDEAFKKMDDPSGRAVGVHQSAYK"
     gene            31024..31100
                     /locus_tag="OO357_000251"
     tRNA            31024..31100
                     /locus_tag="OO357_000251"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31058..31060,aa:Met,seq:cat)
     gene            31109..31193
                     /locus_tag="OO357_000252"
     tRNA            31109..31193
                     /locus_tag="OO357_000252"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31143..31145,aa:Leu,seq:tag)
     gene            31217..31291
                     /locus_tag="OO357_000253"
     tRNA            31217..31291
                     /locus_tag="OO357_000253"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31249..31251,aa:Gln,seq:ttg)
     gene            31307..31383
                     /locus_tag="OO357_000254"
     tRNA            31307..31383
                     /locus_tag="OO357_000254"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31341..31343,aa:Met,seq:cat)
     gene            31432..31506
                     /locus_tag="OO357_000255"
     tRNA            31432..31506
                     /locus_tag="OO357_000255"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31464..31466,aa:Gln,seq:ctg)
     gene            31544..31618
                     /locus_tag="OO357_000256"
     tRNA            31544..31618
                     /locus_tag="OO357_000256"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:31576..31578,aa:Gln,seq:ctg)
     gene            complement(31772..32947)
                     /gene="ubiF"
                     /locus_tag="OO357_000257"
     CDS             complement(31772..32947)
                     /gene="ubiF"
                     /locus_tag="OO357_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-3-methyl-6-methoxy-1,4-benzoquinol
                     hydroxylase"
                     /protein_id="HCR5885328.1"
                     /translation="MTNQPTEIAIVGGGMVGGALALGLAQHGFSVTVIEHAEPAPFVA
                     DSQPDVRISAISAASVSLLKGLGVWDAVQAMRCHPYRRLETWEWETAHVVFDAAELKL
                     PLLGYMVENTVLQQALWQALEAHPKVTLRVPTSLIALHRDNDLQELELKGGETIRAKL
                     VIGADGANSQVRQMAGIGVHAWQYAQSCMLISVQCENDPGDSTWQQFTPDGPRAFLPL
                     FDNWASLVWYDSPTRIRQLQNMNMAQLQAEIAKHFPSRLGYVTPLAAGAFPLTRRHAL
                     QYVQPGLALVGDAAHTIHPLAGQGVNLGYRDVDALIDVLVNARSYGEAWASYPVLKRY
                     QMRRMADNFIMQSGMDLFYAGFSNNLPPLRFVRNLGLMAAERAGVLKRQALKYALGL"
     gene            33093..34517
                     /gene="miaB"
                     /locus_tag="OO357_000258"
     CDS             33093..34517
                     /gene="miaB"
                     /locus_tag="OO357_000258"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HCR5885329.1"
                     /translation="MTKKLHIKTWGCQMNEYDSSKMADLLDATHGYQLTDVAEEADVL
                     LLNTCSIREKAQEKVFHQLGRWKLLKEKNPDLIIGVGGCVASQEGEHIRQRAHYVDII
                     FGPQTLHRLPEMINSVRGDRSPVVDISFPEIEKFDRLPEPRAEGPTAFVSIMEGCNKY
                     CTYCVVPYTRGEEVSRPSDDILFEIAQLAAQGVREVNLLGQNVNAWRGENYDGTTGSF
                     ADLLRLVAAIDGIDRIRFTTSHPIEFTDDIIEVYRDTPELVSFLHLPVQSGSDRILNL
                     MGRTHTALEYKAIIRKLRAARPDIQISSDFIVGFPGETTEDFEKTMKLIADVNFDMSY
                     SFIFSARPGTPAADMVDDVPEEEKKQRLYILQERINQQAMAWSRRMLGTTQRILVEGT
                     SRKSIMELSGRTENNRVVNFEGTPDMIGKFVDVEITDVYPNSLRGKVVRTEDEMGLRM
                     AETPESVIARTRKENDLGVGYYQP"
     gene            34670..35710
                     /locus_tag="OO357_000259"
     CDS             34670..35710
                     /locus_tag="OO357_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HCR5885330.1"
                     /translation="MNIDTREITLEPADNARLLSLCGPFDDNIKQLERRLGIEINRRD
                     NHFKLTGRPICVTAAADILRSLYVDTAPMRGQIQDIEPEQIHLAIKEARVLEQSAESV
                     PEYGKAVNIKTKRGVIKPRTPNQAQYIANILDHDITFGVGPAGTGKTYLAVAAAVDAL
                     ERQEIRRILLTRPAVEAGEKLGFLPGDLSQKVDPYLRPLYDALFEMLGFEKVEKLIER
                     NVIEVAPLAYMRGRTLNDAFIILDESQNTTIEQMKMFLTRIGFNSKAVITGDVTQIDL
                     PRNTKSGLRHAIEVLADVEEISFNFFHSEDVVRHPVVARIVNAYEAWEEAEQKRKAAL
                     AAERKREEQEQK"
     gene            35707..36174
                     /gene="ybeY"
                     /locus_tag="OO357_000260"
     CDS             35707..36174
                     /gene="ybeY"
                     /locus_tag="OO357_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014169133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HCR5885331.1"
                     /translation="MSQVILDLQLACEDNSGLPEESQFQTWLNAVIPQFQEESEVTIR
                     VVDTAESHSLNLTYRGKDKPTNVLSFPFEVPPGMEMSLLGDLVICRQVVEKEAQEQGK
                     PLEAHWAHMVVHGSLHLLGYDHIEDDEAEEMEALETEIMLALGYEDPYIAEKE"
     gene            36264..37142
                     /gene="corC"
                     /locus_tag="OO357_000261"
     CDS             36264..37142
                     /gene="corC"
                     /locus_tag="OO357_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308723.1"
                     /note="CorC(YbeX) belongs to the Cyclin M Mg2+ Exporter
                     (CNNM) family, and was characterized as belonging to a set
                     of three proteins, at least one of which must be present
                     for CorA to function; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family magnesium/cobalt transport protein
                     CorC"
                     /protein_id="HCR5885332.1"
                     /translation="MSDDNSHSSDTISNKKGFFSLLLSQLFHGEPKNRDELLALIRDS
                     GQNDLIDEDTRDMLEGVMDIADQRVRDIMIPRSQMITLKRNQTLDECLDVIIESAHSR
                     FPVISEDKDHIEGILMAKDLLPFMRSDAEAFSMDKVLRQAVVVPESKRVDRMLKEFRS
                     QRYHMAIVIDEFGGVSGLVTIEDILELIVGEIEDEYDEEDDIDFRQLSRHTWTVRALA
                     SIEDFNEAFGTHFSDEEVDTIGGLVMQAFGHLPARGETIDIDGYQFKVAMADSRRIIQ
                     VHVKIPDDSPQPKLDE"
     gene            37167..38705
                     /gene="lnt"
                     /locus_tag="OO357_000262"
     CDS             37167..38705
                     /gene="lnt"
                     /locus_tag="OO357_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HCR5885333.1"
                     /translation="MDFASLIERQRIRLLLALLFGACGTLAFSPYDVWPAAIISLMGL
                     QALTFNRRPLQSAAIGFCWGFGLFGSGINWVYVSIATFGGMPGPVNIFLVVLLAAYLS
                     LYTGLFAGVLSRLWPKTTWLRVAIATPALWQVTEFLRGWVLTGFPWLQFGYSQIDGPL
                     KGLAPLMGVEAINFLLMMVSGLLALALVKRNWRPLVVAVVLFALPFPLRYIQWFTPQP
                     EKTIQVSMVQGDIPQSLKWDEGQLLNTLKIYYNATAPLMGKSSLIIWPESAITDLEIN
                     QQPFLKALDGELRDKGSSLVTGIVDARLNKQNRYDTYNTIITLGKGAPYSYESADRYN
                     KNHLVPFGEFVPLESILRPLAPFFDLPMSSFSRGPYIQPPLSANGIELTAAICYEIIL
                     AEQVRDNFRPDTDYLLTISNDAWFGKSIGPWQHFQMARMRALELARPLLRSTNNGITA
                     VIGPQGEIQAMIPQFAREVLTTNVTPTTGLTPYARTGNWPLWVLTALFGFAAVLMSLR
                     QRRK"
     gene            39102..40010
                     /gene="gltI"
                     /locus_tag="OO357_000263"
     CDS             39102..40010
                     /gene="gltI"
                     /locus_tag="OO357_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter
                     substrate-binding protein GltI"
                     /protein_id="HCR5885334.1"
                     /translation="MQLRKPATAILALALSAGLAQADDAAPAAGSTLDKIAKNGVIVV
                     GHRESSVPFSYYDNQQKVVGYSQDYSNAIVEAVKKKLNKPDLQVKLIPITSQNRIPLL
                     QNGTFDFECGSTTNNVERQKQAAFSDTIFVVGTRLLTKKGGDIKDFADLKGKAVVVTS
                     GTTSEVLLNKLNEEQKMNMRIISAKDHGDSFRTLESGRAVAFMMDDALLAGERAKAKK
                     PDNWDIVGKPQSQEAYGCMLRKDDPQFKKLMDDTIAQVQTSGEAEKWFDKWFKNPIPP
                     KNLNMNFELSDEMKALFKEPNDKALN"
     gene            40180..40920
                     /gene="gltJ"
                     /locus_tag="OO357_000264"
     CDS             40180..40920
                     /gene="gltJ"
                     /locus_tag="OO357_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltJ"
                     /protein_id="HCR5885335.1"
                     /translation="MSIDWNWGIFLQQAPFGNTTYLGWVWSGFQVTIALSICAWIIAF
                     LVGSFFGILRTVPNRFLSGLGTLYVELFRNVPLIVQFFTWYLVIPELLPEKIGMWFKA
                     ELDPNIQFFLSSMLCLGLFTAARVCEQVRAAIQSLPRGQKNAALAMGLTLPQTYRYVL
                     LPNAYRVIVPPMTSEMMNLVKNSAIASTIGLVDMAAQAGKLLDYSAHAWESFTAITLA
                     YVLINAFIMLVMTLVERKVRLPGNMGGK"
     gene            40920..41594
                     /gene="gltK"
                     /locus_tag="OO357_000265"
     CDS             40920..41594
                     /gene="gltK"
                     /locus_tag="OO357_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter permease
                     GltK"
                     /protein_id="HCR5885336.1"
                     /translation="MYEFDWSSIVPSLPYLLDGLVITLKITVTAVVIGILWGTMLAVM
                     RLSSFAPVSWFAKAYVNVFRSIPLVMVLLWFYLIVPGFLQNVLGLSPKNDIRLISAMV
                     AFSMFEAAYYSEIIRAGIQSISRGQSSAALALGMTHWQSMKLIILPQAFRAMVPLLLT
                     QGIVLFQDTSLVYVLSLADFFRTASTIGERDGTQVEMILFAGFVYFVISLSASLLVSY
                     LKRRTA"
     gene            41594..42319
                     /gene="gltL"
                     /locus_tag="OO357_000266"
     CDS             41594..42319
                     /gene="gltL"
                     /locus_tag="OO357_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020245804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate ABC transporter ATP binding
                     protein GltL"
                     /protein_id="HCR5885337.1"
                     /translation="MITLKNVSKWYGHFQVLTDCSTEVKKGEVVVVCGPSGSGKSTLI
                     KTVNGLEPVQQGEITVDGIVVNDKKTDLAKLRSRVGMVFQHFELFPHLSIIENLTLAQ
                     VKVLKRDKAPAREKALKLLERVGLSAHANKFPAQLSGGQQQRVAIARALCMAPIAMLF
                     DEPTSALDPEMINEVLDVMVELANEGMTMMVVTHEMGFARKVANRVIFMDEGKIVEDS
                     PKDAFFDDPKSDRAKDFLAKILH"
     gene            42437..43372
                     /gene="rihA"
                     /locus_tag="OO357_000267"
     CDS             42437..43372
                     /gene="rihA"
                     /locus_tag="OO357_000267"
                     /EC_number="3.2.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-specific ribonucleoside hydrolase
                     RihA"
                     /protein_id="HCR5885338.1"
                     /translation="MALPILLDCDPGHDDAIAIVLALASPELDVKAITSSAGNQTPEK
                     TLRNVLRMLTLLNRTDIPVAGGAVKPLMRELIIADNVHGESGLDGPALPEPTFAPQNC
                     TAVELMAKTLRESAEPVTIVSTGPQTNVALLLNSHPELHSKIARIVIMGGAMGLGNWT
                     PAAEFNIYVDPEAAEIVFQSGIPVVMAGLDVTHKAQIHVEDTERFRAIGNPVSTIVAE
                     LLDFFLEYHKDEKWGFVGAPLHDPCTIAWLLKPELFTTVERWVGVETQGKYTQGMTVV
                     DYYYLTGNKPNATVIVDVDRQGFVDLLADRLKFYA"
     gene            43456..45126
                     /gene="hscC"
                     /locus_tag="OO357_000268"
                     /pseudo
     CDS             43456..45126
                     /gene="hscC"
                     /locus_tag="OO357_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000367910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HscC"
     gene            complement(45186..46637)
                     /gene="djlC"
                     /locus_tag="OO357_000269"
     CDS             complement(45186..46637)
                     /gene="djlC"
                     /locus_tag="OO357_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlC"
                     /protein_id="HCR5885339.1"
                     /translation="MKTCWQILEIESTTQIDIIRQAYLARLPLCHPETDPQGFKALRQ
                     AYEEALRLAVNPVEEADDEEKDATAEHEILRAFRTLLDSESDRFQPSAWQKFIQQLNT
                     WNMEDVDQLRWPLCAIAIEARYLSLNCASLLAERLNWHSFNDSEGMDEEEREAFLEAI
                     QAGDCFDFLSLLEYPVALQNQTVEYYFALERCCRYHPDYVTAFLAMEGPWFIPDDAKL
                     HRKLLRWYSSVQTGMVELIPVAKQWQAEEPESEDARYYQCAQRLYCGEGESLLADLGA
                     YWESYPSTQADNLLLQWSKRHCPDYFALLVMVIEARSMVDAQGQPLKYVPGESARTRL
                     LWAEILHSGKLSPLGQSFIESLFFKRKAWAWWKSRVGSETEEDSPLLDLYRVAEQVVL
                     EAFPKQEMLARLNTRLEGGDAHPLEAIITRMLLTKVKLEPEDEDVDEPTPENHEEKND
                     EGEKPQSITSIIKISLTVLVIGYALGKIAMLFS"
     gene            complement(46634..47341)
                     /locus_tag="OO357_000270"
     CDS             complement(46634..47341)
                     /locus_tag="OO357_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5885340.1"
                     /translation="MNKEEQYLLFALSAPMEILNQSCKPAHDSPKMYTGIKEFDLSSS
                     WGINNRDDLIQTIYQMTDDGHANDLAGLYLTWQRSSPEEWKALIAGGSERGLIYTQFV
                     AQTAMCCGEGGIKAWDYVRMGFLSRAGVLNNWLTEEESLWLQSRVYARAHHYYHSWMH
                     YFSAYSLGRLYWQSSQCEDNASLREALTLYKYDSAGSRMFEELAAGSDRFYATLPWQP
                     LTVQPECPVTLKDVSDL"
     gene            47443..47997
                     /locus_tag="OO357_000271"
     CDS             47443..47997
                     /locus_tag="OO357_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5885341.1"
                     /translation="MNKKLMYIFAIFIVAAITCISQPKKTMLRDKAMVNYAFDYLRSP
                     GSLPLTTAATELSAIHGHSTSQYRLGEFYLHGSDGKPLDYTQARYWYEQSAEQENPRA
                     QSKLGWIYLKGLGVKPDTRKAILWYKEAAEQGYAHAQYTLGLIYRNGTGINVNHYESQ
                     KWLKLAAKQHYKNAERLLAGLPAH"
     gene            complement(48007..49434)
                     /locus_tag="OO357_000272"
     CDS             complement(48007..49434)
                     /locus_tag="OO357_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HCR5885342.1"
                     /translation="MKNCWKILDIEETTDVDIIRRAYLALLPSFHPETDPQGFKQLRQ
                     AYEEALRIAQSPAKSVWQPEEYEVAEHEILLAFRALLASDSERFLPSAWQRFIQQLNY
                     CSMEEIDELRWSLCTIAMNTAHLSFECVVLLAERLRWLQEENVGEIDEEELESFLYAI
                     AKGNVFNFQTILHLPVAVQNDTIDFYQMFARIWSSHPEWLTLYLAQHRAVIIPDDAKL
                     HRNLLRWYSASRLGIPELLDYARSWREAEPDNEDARYYEYAQRVYCGEGESLLAELCY
                     YWREYPSTQADALMLQWCRQHRVYYYPLVVMMIEARDLVNDQGKPLLYVPGDSARTRF
                     HLYEILSDEKLSALGRSLVEMVLHKGRKPRISLTRDTEHPLWPLYLVAKQLVQASQPT
                     EESLMPIVSRLDAEDRCPLEALIIRRLLIQAANFTGQETVEPEPQPQPMPVDDGGLGC
                     LGVIKIIFYIFIFAGLIGKILHLFG"
     gene            complement(49431..50156)
                     /locus_tag="OO357_000273"
     CDS             complement(49431..50156)
                     /locus_tag="OO357_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5885343.1"
                     /translation="MFKDKLYGHGVAKILFALSTPMEIRNECCLPSHSSPKMYLGTCF
                     FDLSSSWGIDDRDDLLRIIHRMIENGHAARLAGFYHRWFRYSPCEWRDYLAELNEQGQ
                     AYAQFVASTAECCGEGGIKAWDYVRMGFLSRMGVLNNWLSEEESLWIQSRIHLRALRY
                     YSNWRQYFAGYTFGRQYWQSPEDDHLPLLREFLARKEYDDSGNDMFYQLFASDDAYYP
                     TLSWQPLADYPTCPETLKDMSDL"
     gene            50301..51278
                     /locus_tag="OO357_000274"
     CDS             50301..51278
                     /locus_tag="OO357_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5885344.1"
                     /translation="MIFTSSCCDNLSIDEIIERAEKGDCEAQYIVGFYYNRDSAIDSP
                     DDEKAFYWLKLAAEQGHCEAQYSLGQKYTEDKSRHKDNEQAIFWLKKAALQGHTFASN
                     ALGWILDRGEDPNYKEAVTWYQIAAESGMSYAQNNLGWMYRNGNGVAQDYTLAFFWYK
                     QAALQGHSYAQDNLADLYKDGEGVAQNKTLAAFWYLKSAQQGNRHAQFQIAWDYNAGE
                     GVDQDYKQAMYWYLKAAAQESVGAYVNIGYMYKHGQGVEKDYQAAFEWFTKAAECNDA
                     TAWYNLAIMYHYGEGRPVDLRQALDLYRKVQSSGTRDVSQEIRETEALL"
     gene            complement(51348..51830)
                     /locus_tag="OO357_000275"
     CDS             complement(51348..51830)
                     /locus_tag="OO357_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon-containing protein"
                     /protein_id="HCR5885345.1"
                     /translation="MNKVAQYYRELVASLNERLRNGERDIDALVEQARERVIKTGELT
                     RTEIDELTRAVRRDLEEFAMSYEESLKEESDSVFMRVIKESLWQELADITDKTQLEWR
                     EIFQDLNHHGVYHSGEVVGLGNLVCEKCHFHLPIYTPEVLTLCPKCGYDQFQRRPFEP
                     "
     gene            52065..54647
                     /gene="leuS"
                     /locus_tag="OO357_000276"
     CDS             52065..54647
                     /gene="leuS"
                     /locus_tag="OO357_000276"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008456064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HCR5885346.1"
                     /translation="MQEQYRPEEIESKVQLHWDEKRTFEVTEDESKEKYYCLSMLPYP
                     SGRLHMGHVRNYTIGDVIARYQRMLGKNVLQPIGWDAFGLPAEGAAVKNNTAPAPWTY
                     DNIAYMKNQLKMLGFGYDWSRELATCTPEYYRWEQKFFTELYKKGLVYKKTSAVNWCP
                     NDQTVLANEQVIDGCCWRCDTKVERKEIPQWFIKITAYADELLNDLDKLDHWPDTVKT
                     MQRNWIGRSEGVEITFNVNDYDNTLTVYTTRPDTFMGCTYLAVAAGHPLAQKAAENNP
                     ELAAFIDECRNTKVAEAEMATMEKKGVDTGFKAVHPLTGEEIPVWAANFVLMEYGTGA
                     VMAVPGHDQRDYEFASKYGLNIKPVILAADGSEPDLSQQALTEKGVLFNSGEFNGLDH
                     EAAFNAIADKLTAMGVGERKVNYRLRDWGVSRQRYWGAPIPMVTLEDGTVMPTPDDQL
                     PVILPEDVVMDGITSPIKADPEWAKTTVNGMPALRETDTFDTFMESSWYYARYTCPEY
                     KEGMLDSEAANYWLPVDIYIGGIEHAIMHLLYFRFFHKLMRDAGMVNSDEPAKQLLCQ
                     GMVLADAFYYVGENGERNWVSPVDAIVERDEKGRIVKAKDAAGHELVYTGMSKMSKSK
                     NNGIDPQVMVERYGADTVRLFMMFASPADMTLEWQESGVEGANRFLKRVWKLVYEHTA
                     KGDVAALNVDALTEDQKALRRDVHKTIAKVTDDIGRRQTFNTAIAAIMELMNKLAKAP
                     TDGEQDRALMQEALQAVVRMLNPFTPHICFTLWQELKGEGDIDNAPWPVADEKAMVED
                     STLVVVQVNGKVRAKITVPVDATEEQVRERAGQEHLVAKYLDGVTVRKVIYVPGKLLN
                     LVVG"
     gene            54662..55243
                     /gene="lptE"
                     /locus_tag="OO357_000277"
     CDS             54662..55243
                     /gene="lptE"
                     /locus_tag="OO357_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HCR5885347.1"
                     /translation="MRYLATLLLSLAVLITAGCGWHLRDTTQVPSTMKVMILDSGDPN
                     GPLSRAVRNQLRLNGVELLDKETTRKDVPSLRLGKVSIAKDTASVFRNGQTAEYQMIM
                     TVNATVLIPGRDIYPISAKVFRSFFDNPQMALAKDNEQDMIVKEMYDRAAEQLIRKLP
                     SIRAADIRSDEEQTSTTTDTPATPARVSTMLGN"
     gene            55243..56274
                     /gene="holA"
                     /locus_tag="OO357_000278"
     CDS             55243..56274
                     /gene="holA"
                     /locus_tag="OO357_000278"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HCR5885348.1"
                     /translation="MIRLYPEQLRAQLNEGLRAAYLLLGNDPLLLQESQDAVRQVAAA
                     QGFEEHHTFSIDPNTDWNAIFSLCQAMSLFASRQTLLLLLPENGPNAAINEQLLTLTG
                     LLHDDLLLIVRGNKLSKAQENAAWFTALANRSVQVTCQTPEQAQLPRWVAVRAKQLNL
                     ELDDAANQVLCYCYEGNLLALAQALERLSLLWPDGKLTLPRVEQAVNDAAHFTPFHWV
                     DALLMGKSKRALHILQQLRLEGSEPVILLRTLQRELLLLVNLKRQSAHTPLRALFDKH
                     RVWQNRRGMMGEALNRLSQPQLRQAVQLLTRTELTLKQDYGQSVWAELEGLSLLLCHK
                     PLADVFIDG"
     gene            56276..56917
                     /gene="nadD"
                     /locus_tag="OO357_000279"
     CDS             56276..56917
                     /gene="nadD"
                     /locus_tag="OO357_000279"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HCR5885349.1"
                     /translation="MKSLQALFGGTFDPVHYGHLKPVETLANLIGLTRVTIIPNNVPP
                     HRPQPEANSVQRKHMLELAIADKPLFTLDERELKRNAPSYTAQTLKEWRQEQGPDVPL
                     AFIIGQDSLLTFPTWYEYETILDNAHLIVCRRPGYPLEMAQPQYQQWLEDHLTHNPED
                     LHLQPAGKIYLAETPWFNISATIIRERLQNGESCEDLLPEPVLTYINQQGLYR"
     gene            56941..57552
                     /locus_tag="OO357_000280"
     CDS             56941..57552
                     /locus_tag="OO357_000280"
                     /EC_number="3.1.3.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin/alpha-ribazole phosphatase"
                     /protein_id="HCR5885350.1"
                     /translation="MRLWLIRHGETQANVDGLYSGHAPTPLTARGIEQAQNLHTLLDD
                     VSFDLVLCSELERAQHTARLVLSDRQLPVHIIPELNEMFFGDWEMRHHRDLMQEDAEN
                     YSAWCNDWQHAIPTNGEGFQAFSQRVERFIARLSEYQHYQNILIVSHQGVLSLLIARL
                     IGMPAESMWHFRVDQGCWSAIDINQKFATLRVLNSRAIGVENA"
     gene            57812..58129
                     /gene="rsfS"
                     /locus_tag="OO357_000281"
                     /gene_synonym="rsfA"
     CDS             57812..58129
                     /gene="rsfS"
                     /locus_tag="OO357_000281"
                     /gene_synonym="rsfA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007373852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HCR5885351.1"
                     /translation="MQGKALQDFVIDKIDDLKGQDIIALDVQGKSSITDCMIICTGTS
                     SRHVMSIADHVVQESRAAGLLPLGVEGENSADWIVVDLGDVIVHVMQEESRRLYELEK
                     LWS"
     gene            58133..58600
                     /gene="rlmH"
                     /locus_tag="OO357_000282"
     CDS             58133..58600
                     /gene="rlmH"
                     /locus_tag="OO357_000282"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HCR5885352.1"
                     /translation="MKLQLVAVGTKMPDWVQTGFTEYLRRFPKDMPFELIEIPAGKRG
                     KNADIKRILDKEGEQMLAAAGKNRIVTLDIPGKPWDTPQLAAELERWKLDGRDVSLLI
                     GGPEGLSPACKAAAEQSWSLSALTLPHPLVRVLVAESLYRAWSITTNHPYHRE"
     gene            58631..60532
                     /gene="mrdA"
                     /locus_tag="OO357_000283"
     CDS             58631..60532
                     /gene="mrdA"
                     /locus_tag="OO357_000283"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-transpeptidase MrdA"
                     /protein_id="HCR5885353.1"
                     /translation="MKLQNSFRDYTAESALFVRRALVAFLGILLLTGVLIANLYNLQI
                     VRFTDYQTRSNENRIKLVPIAPSRGIIYDRNGIPLALNRTIYQIEMMPEKVDNVQQTL
                     DALRSVVDLTDDDIAAFRKERARSHRFTSIPVKTNLTEVQVARFAVNQYRFPGVEVKG
                     YKRRYYPYGSALTHVIGYVSKINDKDVERLNNDGKLANYAATHDIGKLGIERYYEDVL
                     HGQTGYEEVEVNNRGRVIRQLKEVPPQAGHDIYLTLDLKLQQYIETLLAGSRAAVVVT
                     EPRTGGVLALVSTPSYDPNLFVDGISSKDYSALLNDPNTPLVNRATQGVYPPASTVKP
                     YVAVSALSAGVITRNTTLFDPGWWQLPGSEKRYRDWKKWGHGRLNVTRSLEESADTFF
                     YQVAYDMGIDRLSEWMGKFGYGHYTGIDLAEERSGNMPTREWKQKRFKKPWYQGDTIP
                     VGIGQGYWTATPIQMSKALMILINDGIVKVPHLLMSTAEDGKQVPWVQPHEPPVGDIH
                     SGYWELAKDGMYGVANRPNGTANKYFASAPYKIAAKSGTAQVFGLKANETYNAHKIAE
                     RLRDHKLMTAFAPYNNPQVAVAMILENGGAGPAVGTLMRQILDHIMLGDNNTDLPAEN
                     PAVAAAEDH"
     gene            60535..61647
                     /gene="mrdB"
                     /locus_tag="OO357_000284"
                     /gene_synonym="rodA"
     CDS             60535..61647
                     /gene="mrdB"
                     /locus_tag="OO357_000284"
                     /gene_synonym="rodA"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004885876.1"
                     /note="rod shape-determining protein RodA; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase MrdB"
                     /protein_id="HCR5885354.1"
                     /translation="MTDNPNKKTFWDKVHLDPTMLLILLALLVYSALVIWSASGQDIG
                     MMERKIGQIAMGLVIMVVMAQIPPRVYEGWAPYLYIICIILLVAVDAFGAISKGAQRW
                     LDLGIVRFQPSEIAKIAVPLMVARFINRDVCPPSLKNTGIALVLIFMPTLLVAAQPDL
                     GTSILVALSGLFVLFLSGLSWRLIGVAVVLVAAFIPILWFFLMHDYQRQRVMMLLDPE
                     SDPLGAGYHIIQSKIAIGSGGLRGKGWLHGTQSQLEFLPERHTDFIFAVLAEELGLVG
                     ILILLALYILLIMRGLWIAARAQTTFGRVMAGGLMLILFVYVFVNIGMVSGILPVVGV
                     PLPLVSYGGSALIVLMAGFGIVMSIHTHRKMLSKSV"
     gene            61658..62746
                     /gene="rlpA"
                     /locus_tag="OO357_000285"
     CDS             61658..62746
                     /gene="rlpA"
                     /locus_tag="OO357_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic peptidoglycan transglycosylase RlpA"
                     /protein_id="HCR5885355.1"
                     /translation="MRKQWLGICIAAGMLAACTSDDGQQQTVSVPQPAVCNGPIVEIS
                     GADPRFEPLNATANQDYQRDGKSYKIVQDPSRFSQAGLAAIYDAEPGSNLTASGEAFD
                     PTKLTAAHPTLPIPSYARITNLANGRMIVVRINDRGPYGNDRVISLSRAAADRLNTSN
                     NTKVRIDPIIVAQDGSLSGPGMACTTVAKQTYALPAPPDLSGGAGTSSVSGPQGDILP
                     VSNSTLKSEDPTGAPVTSSGFLGAPTTLAPGVLEGSEPTPAPQPVVTASSTTPATSPA
                     MVTPQAASQSASGNFMVQVGAVSDQARAQQYQQQLGQKFGVPGRVTQNGAVWRIQLGP
                     FASKAEASTLQQRLQTEAQLQSFITTAQ"
     gene            62885..64096
                     /gene="dacA"
                     /locus_tag="OO357_000286"
     CDS             62885..64096
                     /gene="dacA"
                     /locus_tag="OO357_000286"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase DacA"
                     /protein_id="HCR5885356.1"
                     /translation="MNTIFSARIMKRLALTTALCTAFISAAHADDLNIKTMIPGVPQI
                     DAESYILIDYNSGKVLAEQNADVRRDPASLTKMMTSYVIGQAMKAGKFKETDLVTIGN
                     DAWATGNPVFKGSSLMFLKPGMQVPVSQLIRGINLQSGNDACVAMADFAAGSQDAFVG
                     LMNSYVNALGLKNTHFQTVHGLDADGQYSSARDMALIGQALIRDVPNEYSIYKEKEFT
                     FNGIRQLNRNGLLWDNSLNVDGIKTGHTDKAGYNLVASATEGQMRLISAVMGGRTFKG
                     REAESKKLLTWGFRFFETVNPLKVGKEFASEPVWFSDSDRASLGVDKDVYLTIPRGRM
                     KDLKASYVLNSSELHAPLQKNQVVGTINFQLDGKTIEQRPLVVLQEIPEGNFFGKIID
                     YIKLMFHHWFG"
     gene            64207..64470
                     /locus_tag="OO357_000287"
     CDS             64207..64470
                     /locus_tag="OO357_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbeD family protein"
                     /protein_id="HCR5885357.1"
                     /translation="MKTKLNELLEFPTPFTYKVMGQALPELVDQVVEVVQRHAPGDYT
                     PTVKPSSKGNYHSVSITINATHIEQVETLYEELGKIDIVRMVL"
     gene            64571..65212
                     /gene="lipB"
                     /locus_tag="OO357_000288"
     CDS             64571..65212
                     /gene="lipB"
                     /locus_tag="OO357_000288"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415163.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HCR5885358.1"
                     /translation="MYQDKILVRQLGLQPYEPISRAMHEFTDTRDNSTLDEIWLVEHY
                     PVFTQGQAGKAEHILMPGDIPVIQSDRGGQVTYHGPGQQVMYVLLNLKRRKLGVRELV
                     TLLEQTVVNTLAELGIEAHPRADAPGVYVGEKKICSLGLRIRRGCSFHGLALNVNMDL
                     SPFLRINPCGYAGMEMAKISQWKPEATTNNIAPRLLENILALLNNPDFEYITA"
     gene            65471..66424
                     /locus_tag="OO357_000289"
     CDS             65471..66424
                     /locus_tag="OO357_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415162.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5885359.1"
                     /translation="MDSNNQIEPCLSRKSSEGKPQIFTTLRNIDLNLLTIFEAVYVHK
                     GIVNAAKVLNLTPSAISQSIQKLRVIFPDPLFIRKGQGVTPTAFAMHLHEYISQGLES
                     ILGALDIEGSYDKQRTITIATTPSVGALVLPVIYRAIKTHYPQLLLRNPPISDAENQL
                     SQFQTDLIIDNMFCTNRTVQHHVLFTDNMVLICRKGNPLLSLEDDRETIDNAAHVLLL
                     PEGQNFSGLRQRVQEMFPDRQINFTSYNILTIAALVANSDMLAIIPSRFYNLFSRCWP
                     LEKLPFPSLNEEQIDFSIHYNKFILRDPILHGVIDVIRNAF"
     gene            66633..67598
                     /gene="lipA"
                     /locus_tag="OO357_000290"
     CDS             66633..67598
                     /gene="lipA"
                     /locus_tag="OO357_000290"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HCR5885360.1"
                     /translation="MSKPIVMERGVKYRDADKMALIPVKNVATEREALLRKPEWMKIK
                     LPADSTRIQGIKAAMRKNGLHSVCEEASCPNLAECFNHGTATFMILGAICTRRCPFCD
                     VAHGRPVAPDANEPVKLAQTIADMALRYVVITSVDRDDLRDGGAQHFADCITAIREKS
                     PQIKIETLVPDFRGRMDRALDILTATPPDVFNHNLENVPRIYRQVRPGADYNWSLKLL
                     ERFKEAHPEIPTKSGLMVGLGETNEEIIEVMRDLRRHGVTMLTLGQYLQPSRHHLPVQ
                     RYVSPDEFDEMKAEALAMGFTHAACGPFVRSSYHADLQAKGMEVK"
     gene            complement(67699..67902)
                     /gene="tatE"
                     /locus_tag="OO357_000291"
     CDS             complement(67699..67902)
                     /gene="tatE"
                     /locus_tag="OO357_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatE"
                     /protein_id="HCR5885361.1"
                     /translation="MGEISITKLLVVAALVVLLFGTKKLRTLGGDLGAAIKGFKKAMN
                     DDDAAAKKGADVDLQAEKLSHKE"
     gene            complement(68031..68819)
                     /locus_tag="OO357_000292"
     CDS             complement(68031..68819)
                     /locus_tag="OO357_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706584.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deaminated glutathione amidase"
                     /protein_id="HCR5885362.1"
                     /translation="MLVAAGQFAVTSVWEKNAEICASLMAQAAENDVSLFVLPEALLA
                     RDDHDADLSVKSAQLLEGEFLGRLRRESKRNMMTTILTIHLPSTPGRAWNMLVALQAG
                     NIVARYAKLHLYDAFAIQESRHVDAGNEIAPLLEVEGMKVGLMTCYDLRFPELALAQA
                     LQGAEILVLPAAWVRGPLKEHHWSTLLAARALDTTCYMVAAGECGNKNIGQSRIIDPF
                     GVTIAAASEMPALIMAEVTPERVRQVRAQLPILNNRRFAPPQLL"
     gene            68912..69295
                     /gene="crcB"
                     /locus_tag="OO357_000293"
     CDS             68912..69295
                     /gene="crcB"
                     /locus_tag="OO357_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HCR5885363.1"
                     /translation="MLQLLLAVFIGGGTGSVARWLLSMRFNPLHQAIPLGTLAANLIG
                     AFIIGMGFAWFSRMTNIDPVWKVLITTGFCGGLTTFSTFSAEVVFLLQEGRFGWALLN
                     VFVNLLGSFAMTALAFWLFSASTVH"
     gene            complement(69349..69558)
                     /gene="cspE"
                     /locus_tag="OO357_000294"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(69349..69558)
                     /gene="cspE"
                     /locus_tag="OO357_000294"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5885364.1"
                     /translation="MSKIKGNVKWFNESKGFGFITPEDGSKDVFVHFSAIQTNGFKTL
                     AEGQRVEFEITNGAKGPSAANVIAL"
     gene            complement(69733..70293)
                     /gene="pagP"
                     /locus_tag="OO357_000295"
     CDS             complement(69733..70293)
                     /gene="pagP"
                     /locus_tag="OO357_000295"
                     /EC_number="2.3.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) palmitoyltransferase PagP"
                     /protein_id="HCR5885365.1"
                     /translation="MNVSKYVAIFSFVFIQLISVGKVFANADERMTTFRENIAQTWQQ
                     PEHYDLYIPAITWHARFAYDKEKTDRYNERPWGGGFGLSRWDEKGNWHGLYAMAFKDS
                     WNKWEPIAGYGWESTWRPLADENFHLGLGFTAGVTARDNWNYIPLPVLLPLASVGYGP
                     VTFQMTYIPGTYNNGNVYFAWMRFQF"
     gene            70882..72267
                     /gene="dcuC"
                     /locus_tag="OO357_000296"
     CDS             70882..72267
                     /gene="dcuC"
                     /locus_tag="OO357_000296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5885366.1"
                     /translation="MLTFIELLIGVVVIVGVARYIIKGYSATGVLFVGGLLLLIISAI
                     MGHKVLPSSQASTGYSATDIVEYVKILLMSRGGDLGMMIMMLCGFAAYMTHIGANDMV
                     VKLASKPLQYINSPYLLMIAAYFVACLMSLAVSSATGLGVLLMATLFPVMVNVGISRG
                     AAAAICASPAAIILAPTSGDVVLAAQASEMSLIDFAFKTTLPISIAAIIGMAIAHFFW
                     QRYLDKKEHISHEMLDVSEITTTAPAFYAILPFTPIIGVLIFDGKWGPQLHIITILVI
                     CMLIASILEFLRSFNTQKVFSGLEVAYRGMADAFANVVMLLVAAGVFAQGLSTIGFIQ
                     SLISIATSFGSASIILMLVLVILTMLAAVTTGSGNAPFYAFVEMIPKLAHSSGINPAY
                     LTIPMLQASNLGRTLSPVSGVVVAVAGMAKISPFEVVKRTSVPVLVGLVIVIVATELM
                     VPGTAAAVTGK"
     gene            complement(72308..72649)
                     /locus_tag="OO357_000297"
                     /pseudo
     CDS             complement(72308..72649)
                     /locus_tag="OO357_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308686.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
     gene            complement(72987..73496)
                     /locus_tag="OO357_000298"
     CDS             complement(72987..73496)
                     /locus_tag="OO357_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5885367.1"
                     /translation="MKSANEINQWMTPKQITELDGMPGTIQGVHKRAKKEGWPKRSQE
                     GRRGPGVEYIPAIPAMQKIAIEEMNADTLKMFSLLINKLGENEVREIELNMAKYGLSG
                     LMQERPSISVDTLLATLGIDRQTLQTALALHKLPPETRQEILSMYGVHKQEEPVAPLL
                     EPQDAKKAV"
     gene            73659..73904
                     /locus_tag="OO357_000299"
     CDS             73659..73904
                     /locus_tag="OO357_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013024337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5885368.1"
                     /translation="MRKMSNDWHRADVIAALKKTGTPLAKLSRRSGYSTGTLANALVR
                     PWPKGERIIADALNMKPEEIWPSRYLRDHNERVDISN"
     gene            73879..>75508
                     /locus_tag="OO357_000300"
     CDS             73879..>75508
                     /locus_tag="OO357_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mu transposase C-terminal domain-containing
                     protein"
                     /protein_id="HCR5885369.1"
                     /translation="MREWISAIECMGLSGLPGSLMGVHKRAQREGWLKRRREGIRGVA
                     YEYHISSLPNIARAEVLLQQGKFKTSQGCFEIARPTLEAHDYDREALWSKWDKASDSQ
                     RSLAEKWLPSIQATDEMLNQGISTKTAFATVAGHYQVSASTLRDKYYQVQKFAKPDWA
                     AALVDGRGASRRNVHKSEFDEDAWQFLIADYLRPEKPAFRKCYERLELAAREHGWSIP
                     SRATAFRRIQQLDEAMVVACREGEHALMHLIPAQQRTVEHLDAMQWINGDGYLHNVFV
                     RWFNGDVIRPKTWFWQDVKTRKILGWRCDVSENIDSIRLSFMDVVTRYSIPEDFHITI
                     DNTRGAANKWLTGGAPNRYRFKVKEDDPKGLFLLMGAKMHWTSVVAGKGWGQAKPVER
                     AFGVGGLEEYVDKHPALAGAYTGPNPQAKPDNYGDHAVDAELFLKTFAEGVAMFNART
                     GRETEMCGGKLSFDDVFEREYARTIVRKPTEEQKRMLLLPAEAVNVSRKGEFTLKVGG
                     SLKGAKNVYYNMALMNAGVKKVVVRFDPQQLHSTV"
BASE COUNT        18586 a        18959 c        19182 g        18781 t
ORIGIN      
        1 tattggatta gatatgttaa caattaatta ttcttaatat atcaataaag tatatattac
       61 tctctttact atctgttttt attgcggtag cgatactaat aacaaaattc ggaatttatt
      121 tagatgctgg aattagaaaa agggatgacg gctttctcac cttaccctga agtttcacga
      181 aagtgtgtaa ggaagtatgc aatctaactg gagatttctg atgatctagt cgcttagagc
      241 gacaatgaag gtgaccgcat cctgggtagc cctatgaaag gatactattg aaacgtgatg
      301 ctaaaacagt gatgaaatga ataatacgat ggaaacgtct tccatgttaa ttatcatgga
      361 agacgttgat gttgaacacc taaaccagcc ccctgatacg aggctggctt aagcgcgcag
      421 gcaatcagta aaaatcaccg gttgcctttt cggcctgatc catccacacc ggtttatcgc
      481 tggttttcga ccagacgcgg tgcaggtagc tgtaaaagcg ggcgcggtct ttccagaaaa
      541 gcatgaaagg cagcgccaga acgcccgctg caaccgcata tgtacggcgt aaaaagataa
      601 gccatgcagg atattctctg tagagttcca tacttttctc ccccttttgt atctacccgg
      661 tgaatagcac cggaaaaatg aatttgttta tctgatgaaa atagtaccgc cttttgtgta
      721 attttactac tcatccgacc acttattttt gcttattgat ggtttattta cattcacact
      781 gtaattaaat tacacaaaag ttaaattaat actaaccatc agttaaatca tggcctttgc
      841 catttttata ctttttttac accccgtccg cagatttttg cgaaatcttt gcagccagaa
      901 ttctactctt ccggtatcac ttttaggcca ctggaggtgc actgtgagtg caggcgtgat
      961 agccggcgtg ttgctggtgt ttttattact gggctatttg gtttatgccc tgatcaatgc
     1021 ggaggcgttc tgatggctgc gcaagggttc ttactgattg ccacgttttt actggtatta
     1081 atggtgctgg cgcgtccttt aggcagcggg ctggcgcggc tgattaatga cattcctctt
     1141 ccaggtacag cgggcgttga acgtatactc tttcgtttac ctggcgtctc tgaccatgag
     1201 atgaactgga agcaatatct ttgtgccatt ctcggcctga acatgctggg gctggcggtg
     1261 ctgtttttta tgttgctcgg tcagcactat ctgccgctta atccacagca gttgccgggg
     1321 ctgtcgtggg atctggcgct gaataccgcc gtcagctttg tcacgaatac caactggcaa
     1381 tcttatagcg gtgaaaccac gttgagctat ttcagccaga tggcaggctt aacggtacaa
     1441 aactttcttt ctgccgccag cgggattgcg gtgatttttg ccttcatccg tgcgtttact
     1501 cgccagagca tgagcacact cgggaatgcc tgggtcgatc tgctacgcat cacgttatgg
     1561 gtgctagtcc ctgtggcgtt gttgatcgca ctgtttttta ttcaacaagg tgcgcagcaa
     1621 aactttctgc cctatcaggc agtgaatacc gttgaaggag cgcaacagct gttacccatg
     1681 ggaccagtag cttctcagga agcgatcaag atgctcggta ctaacggcgg tggcttcttt
     1741 aatgccaact cgtcgcatcc gtttgaaaac ccaaccgcac tgaccaactt cgtgcagatg
     1801 ctggcgatct tcttgatccc aacagcgctg tgctttgcct ttggtgaagt gacgggcgat
     1861 cgccgccagg ggcgcatgtt gctgtgggcg atgtcagtga tttttgtcat ctgcgtaggc
     1921 gtggttatgt gggcagaagt tcagggtaat cctcatctgc tggcgctcgg cgcagacagc
     1981 agcatcaata tggaaggtaa agagagccgt ttcggcgtgc tggtcagtag cctgtttgcg
     2041 gtcgtgacta cggcggcgtc ctgtggcgcg gtgattgcga tgcatgattc gtttaccgct
     2101 ctcggcggca tggtgccgat gtggctaatg caaattggtg aagtggtgtt cggcggtgtc
     2161 ggttctggtc tttacggcat gatgctgttt gtcctgctgg cggtgtttat tgccgggctg
     2221 atgattggtc gtacaccgga atatctgggt aaaaaaatag acgtacgcga gatgaaactg
     2281 actgcactgg cgattctggt tactccaaca ctggtactga tgggcgcggc gttggcgatg
     2341 atgaccgacg ccggacgtag cgccatgctt aaccctagcc cgcatggttt tagcgaagtg
     2401 ctgtacgccg tgtcgtccgc cgctaacaac aacggcagcg cctttgccgg attaagcgcc
     2461 aactctccgt tctggaactg tttactggcg ttctgcatgt ttgtcggtcg cttcggggtg
     2521 attatcccgg tgatggcaat tgccggttcg ctggtgagta aaaagagcca acccgccagc
     2581 tccggcacgc tgccaacgca cggcccgctg tttgttggcc tgttaatcgg caccgtgttg
     2641 ctggttggcg cactgacctt tatccctgcc ctggcgcttg gtccggtggc ggaatatctc
     2701 tcctgatgat attgagtgag cactgaatat gagtcgtaaa caactggcgc tattcgaacc
     2761 aacacttgtc gttcaggcgc tgaaagaagc ggtgaaaaaa ttaaacccgc aggcgcaatg
     2821 gcgcaatccg gtgatgttta tcgtctggat cggcagtctg ctgaccacct gtattagcat
     2881 cgcgatggca agcggtgcga tgcctggcaa tgcgctgttt agcgcggcaa ttagcggctg
     2941 gctgtgggtc accgtactgt tcgctaattt cgccgaggcg ctggcagaag gccgcagtaa
     3001 agcgcaggcc aacagtctta aaggggtgaa aaaaactgcc tttgcccgca agctgcgtga
     3061 gccgaaatat ggcgctgcgg cggacaaagt tcctgccgat caactgcgta aaggtgacat
     3121 cgtgctggta gaagctggcg atattatccc ctgcgatggt gaagtcattg aaggcggcgc
     3181 atcggtcgat gaaagcgcca tcaccgggga atcggcaccg gtgatccgtg aatccggcgg
     3241 cgattttgcc tccgttaccg gcggcacgcg tattctttct gactggctgg tgattgagtg
     3301 tagcgttaac cccggtgagg catttctgga tcggatgatc gcgatggtgg aaggcgcaca
     3361 gcgacgcaaa acaccgaacg agattgccct gactatcctg ctgattgctc tgaccatcgt
     3421 ctttttactg gcaaccgcca cgctgtggcc gttttccgcg tggggcggta atgccgtgag
     3481 tgtaacggta ctggtggcgc tgctggtctg tctgatccca accactattg gtggcctgtt
     3541 gtcagcgatc ggcgtcgccg ggatgagtcg gatgctgggc gcgaatgtga ttgccaccag
     3601 cggacgtgca gttgaagcgg caggtgacgt tgacgttctg ctactggata aaaccggcac
     3661 catcacactc ggtaaccgtc aggcgtcgga gtttatcccc gcgcagggcg tggatgaaaa
     3721 aacgctggct gacgccgcac aactggcttc gctggctgat gaaacgccgg aaggtcgcag
     3781 tattgtgatc ctcgccaagc aacgttttaa cctgcgcgag cgcgatgtgc agtcgctcca
     3841 tgccaccttt gtaccgttta ctgcgcaaag ccggatgagc gggatcaaca tcgacaaccg
     3901 catgatccgt aaaggttctg tcgatgccat tcgccgccat attgaggcta acggcggtca
     3961 cttccctgcc gatgttgatc aaaaagtcga tcaggttgcg cgtcaaggag ccacgctgct
     4021 ggtggtggtg gaaggttctc gtgtgctggg cgttattgcg ctgaaagata tcgtcaaagg
     4081 cggtattaaa gagcgcttcg cccagctgcg caaatgggca ttaaaacggt gatgattacc
     4141 ggcgataacc gtctgactgc cgccgcgata gctgcggaag cgggtgtcga tgattttctc
     4201 gccgaagcga caccggaggc caagctggca ttgatccgtc agtatcaggc ggaaggtcgt
     4261 ttggtagcga tgaccggcga cagcaccaac gatgctacgg cgctggcgca ggcagatgtc
     4321 gcggtggcga tgaactccgg cacccaggcg gctaaagagg cgggcaatat ggtggatctc
     4381 gactctaacc cgaccaagtt gatcgaggtg gtgcacattg gcaaacagat gctgatgacc
     4441 cgtggctcgc tgaccacctt cagcattgcc aacgatgtgg cgaaatactt cgccattatt
     4501 ccggcggcgt tcgcggcaac atatccgcag ttaaatgcgc tgaacatcat gcgcctgcat
     4561 tcgcccgact ccgccattct cagtgcggtc attttcaacg ccttgattat cgtctttttg
     4621 attcctctgg cgttaaaagg cgtgagttat aaaccgctta ccgcttctgc catgttgcgc
     4681 cgtaacttat ggatttacgg tctgggtggg ctgctggtgc cgtttattgg tatcaaagtc
     4741 attgatttac tgctaaccgt ttgtagtctg gtgtgaggtt taccatgagt ggattacgtc
     4801 cggcattatc gacatttctc tttctgttat tgattactgg cggcgtttac ccgctgctga
     4861 ccaccgcact ggggcaatgg tggtatccct ggcaggccaa cggttcgttg attcgtgaag
     4921 gtgatacggt gcgcggttcg gcattaatcg ggcaaaattt taccggcaac ggctattttc
     4981 atggtcgccc gtcggcaaca gcagaaatgc cctataatcc acaggcttct ggcgggagca
     5041 atctggcagt cagtaacccg gagctggata aacagatagc cgcacgcgtt gccgcattac
     5101 gggccgctaa cccggacgcc agcacgaacg ttccggttga actggtgacg gcgtcggcaa
     5161 gcggactgga caataacatc accccgcaag cggcggcctg gcagatccca cgcatcgcaa
     5221 aagcgcgtaa tctcagcgtt gaacagctca cgcagctgat cgcaaaatac agccaacaac
     5281 cgctggtgaa atatatcggc cagccggtcg tcaacattgt cgaactcaat ctggcactgg
     5341 ataagcttga tgaataacga acccttacgt cccgaccccg accgtctgct ggaacaaact
     5401 gccgcgccgc atcgggggaa gctgaaagtt ttcttcagtg cctgtgcagg cgtcgggaag
     5461 acctgggcga tgctggcaga agcccagcga ctgcgggcgc aagggctgga tattgtggtt
     5521 ggtgtggtag aaacccatgg gcggaaagat accgccgcca tgcttgaagg gctggctgtt
     5581 ctgcctctga aacgccaggc gtaccgtggg cggcatatca gcgagtttga tctcgatgcc
     5641 gccctcgccc gccgtccggc gctgatctta atggacgaac tggcgcacag taatgcgcca
     5701 ggttctcgtc atcctaaacg ctggcaggat atcgaagaac tgctggaagc tggcattgat
     5761 gttttcacaa ccgtcaacgt tcagcatctg gaaagtctga atgatgtggt cagcggcgtc
     5821 accggaattc aggtacggga aaccgtgccc gatccttttt tcgatgccgc cgacgacgtg
     5881 gtgctggtgg acttgccccc ggacgatctg cgccagcggc tgaaagaagg caaagtctat
     5941 attgccgggc aggcggagcg cgccattgaa cattttttcc gcaaaggcaa tctgatcgcc
     6001 ctgcgcgaac tggcactgcg ccgtactgcc gatcgcgttg atgagcaaat gcgcgcctgg
     6061 cgggggcatc ctggcgaaga gaaagtctgg cacacgcgcg acgcgatcct tttatgcatc
     6121 ggccataaca ccggcagcga aaactggtcc gcgcggcggc gcggctgacg tcacggctgg
     6181 ggagcgtctg gcacgcggtg tatgttgaaa cccctgccct gcatcgctta ccggagaaaa
     6241 aacgtcgggc aattctcagc gccttacgtc tggcgcagga gctgggcgcg gagacggcaa
     6301 cactttctga tccagcggaa gagaaagcgg tagtgcgtta tgcccgtgaa cataatctcg
     6361 gcaagattat tctcggtcgc ccagcctcgc gccgctggtg gcgtcgggaa acatttgctg
     6421 accgactggc gcgcatcgcc cccgatctcg atcaggtgct ggtcgcgctt gatgaaccac
     6481 ccgcccgcac gattaacaac gcgccggata gccgctcatt taaagacaag tggcgggtac
     6541 agattcaggg atgcgtggtt gccgccgcgt tatgcgccgt tatcacctta attgccatgc
     6601 agtggctgat ggcgtttgat gccgccaacc tggtgatgct gtatctgctt ggcgtggtgg
     6661 tggcgctatt ttatggacgc tggccttcgg tggttgccac cgtcattaat gtggtgagtt
     6721 tcgatctctt ttttatcgcc ccacgcggca cgctcgccgt ctctgatgtg caatatctgc
     6781 tgaccttcgc ggtgatgtta accgtcgggc tggtgatcgg gaaccttact gctggtgtgc
     6841 gttatcaggc gcgggtagcc cgttaccgcg agcaacgcac acggcactta tatgaaatgt
     6901 cgaaagctct ggcggtgggc cgcagtccgc aggacatcgc cgccaccagc gaacaattta
     6961 ttgcctccac gtttcatgcc cgcagtcagg tgttgttgcc cgatgacaac ggtaaattgc
     7021 agccgttaac acatccgcaa ggaattacgc catgggacga tgccatcgcg cagtggagtt
     7081 ttgataaagg cctgcctgcg ggcgcgggca ccgacacgtt acccggtgta ccgtaccaga
     7141 ttttgccgct aaaaagcggc gagaaaacct acgggctggt ggtggtggag ccggggaatc
     7201 tgcgccagtt gatgatcccg gaacagcagc gcctgctgga gacttttacg ctgttagtcg
     7261 ccaatgccct tgagcggctg acgctaaccg ccagcgaaga acaggcgcgg atggcaagcg
     7321 aacgtgaaca gatccgcaac gccctgctgg cagcgctttc gcatgattta cgcacgccgc
     7381 ttacggtgct gtttggtcag gcagaaatct taacgctcga tctggcaagc gaaggatcac
     7441 cccacgcccg ccaggccagc gagattcgtc agcatgtgct gaacactacc cgactggtga
     7501 ataatctact ggatatggcg cgaattcagt ccggcggctt taatttgaag aaagagtggt
     7561 taacgctgga agaagtagtc ggcagcgcgc tgcaaatgcc ggaaccgggt ttatcgtcgc
     7621 ccatcaatct ttctctgcca gaaccgctga ccttaatcca cgttgacggg ccactctttg
     7681 aacgggtgct gattaatctg ctggagaacg cggtgaaata tgcgggtgcg caggccgaaa
     7741 ttggtatcga tgcccacgtt gagggcgaaa atctacaact ggatgtctgg gataacggcc
     7801 ccggtcttcc gccaggccag gagcagacga tatttgataa gtttgctcgc gggaataaag
     7861 agtcggcggt gccgggtgtc gggcttgggt tggcaatttg tcgggcgatt gtggatgtac
     7921 acggcggcac cattaccgcg ttcaaccgac cggaaggtgg tgcctgtttt cgtgttacac
     7981 ttccccagca aaccgctcct gaacttgaag actttcatga ggatatgtga caaacgttct
     8041 gattgttgaa gatgaacagg ctattcgtcg ctttctgcgc acggcgctgg agggcgacgg
     8101 gatgcgcgtc tttgaggccg aaactctgca acgcggcttg ctggaagcgg caacccgtaa
     8161 gccagatttg attattctcg atctcggcct gcccgatggt gatgggattg agtttatccg
     8221 cgacctgcgc cagtggagcc cggtgccggt gattgtgctt tccgcacgca gcgaagagag
     8281 cgacaagatc gccgcgctgg atgccggagc ggatgattat ctgagtaagc cgtttggtat
     8341 tggcgaattg caggcccgtc tgcgcgtcgc tttacgccgc cactccgcca ctccgccacc
     8401 gccgcgcccg atccgctggt gaaattttcc ggtgttaccg tcgatttagc cgcccgcgtg
     8461 attcaccggg gtgaggaaga ggtgcatctc acaccaattg agttccgcct gctggcggtg
     8521 ctgctcaaca atgccggaaa agtactcacc cagcgccagc tccttaacca ggtgtggggg
     8581 ccaaacgcgg tcgaacacag tcactatttg cgtatttata tgggacatct gcgacaaaaa
     8641 ctggaacagg atccggcccg gcctcgccac ttcattactg aaaccggtat tgggtatcgg
     8701 tttatgcttt gaattgttat tcaataaatt cgcttaaata gtgattaatt tattattaaa
     8761 cctgcgccga atattattat ttggcaggtt taatttctta acgcaaatat aaaacacgaa
     8821 attacaacat ttaagtaaca aaacagcatc taatatggcg atattcataa ttaatgattt
     8881 attatctggg gtatgatcgt tttttgttga tcttcttcac agattatagc catttcatgg
     8941 atagaataac tctaccttca actgacacag caagcggtaa aggtaaatgg aaaataataa
     9001 ccgcttaatg cctcatataa ggcggacaac ccatatcatg aagtttgccc atcgtaatag
     9061 ttttgacttt catttcttta atgcccgcta gtcttctgac taaagggcac cccaacgtac
     9121 aggtctccct gactttaagc attacaggtt aatacctgta ttcctcggtg ctcatatact
     9181 gctaaccctt ttaaactcta aataattcga gtcgcagcac ttgcaacttg aggtatgacg
     9241 agtatagcca gttaccgggc tggtctgggt tattgcatct gcaaaaagca aactactgat
     9301 ttatttatca gcggtggagc tttgcttttt ttccgacgtg atcgatttct cctttgagaa
     9361 attgaggacc tgctattacc tgaaataaag agatgaataa aatgtcagaa ttaaaaattg
     9421 ccgttagccg ttcttgcccg gattgttttt ccactcatcg tgcatgcgtg aatattgacg
     9481 aaagtaatta tattgacgtt gccgccatta ttttatcagt caatgatgtt gaacgtggaa
     9541 aactcgataa aatagacgct actggctatg gcattcctgt ttttattgca acggaaaatg
     9601 aagaacgtgt accagcagaa tatctgccac gtatttctgg tgtcttcgag cattgcgaat
     9661 cacggaaaga gttttatgga cgtcagttag aaaccgctgc tagccattat gaaacgcaac
     9721 tgcgcccgcc cttcttccgc gcactggtcg attatgtcaa tcagggcaat agcgcttttg
     9781 actgcccggg tcatcaggga ggtgagtttt tccgtcgtca tccggcaggg aatcagttcg
     9841 tggagtactt cggagaaacg ctgttccgtt ccgacttatg caacgcagac gtagcaatgg
     9901 gcgatctgct gattcacgaa ggcgcgccat gcattgcaca gcaacatgcg gcaaaagtgt
     9961 ttaatgccga taaaacatac ttcgttctaa atggcacttc atcttctaac aaagtggttt
    10021 taaacgccct gctaacaccg ggtgatctgg tgctgtttga tcgcaataac cacaaatcta
    10081 accaccacgg agcgttgcta caggctggtg caacaccggt ttatctggaa acggcacgta
    10141 acccgtatgg ctttatcggt ggcattgatg cgcactgttt tgaagaaagt tacctgcgtg
    10201 agctgatcgc ggaagtcgca ccgcagcggg caaaagaggc tcgtcctttc cgcctcgctg
    10261 tgattcagtt aggcacctac gacggtacga tttataacgc ccgccaggtg gtggataaaa
    10321 ttggtcatct gtgtgactac attctgtttg actccgcatg ggttggctat gagcaattta
    10381 tcccgatgat ggctgactgt tcgccgctgt tgctggatct taatgagaac gatccgggta
    10441 ttctagttac gcaatccgta cataaacagc aggctggttt ttctcaaact tcacaaatcc
    10501 ataaaaaaga cagccacatc aaagggcaac agcgttatgt accgcacaaa cgcatgaaca
    10561 acgcctttat gatgcacgcc tccaccagcc cgttctatcc gctgtttgcc gcactggata
    10621 tcaacgccaa aatgcatgaa ggtgtcagcg gtcgtaatat gtggatggat tgtgtggtaa
    10681 atggcattaa tgcccgcaaa ctgatcctcg ataactgtca gcatattcgt ccgttcgtac
    10741 ctgaactggt ggatggtaaa ccctggcagt cgtatgaaac agcgcaaatt gcggttgatc
    10801 tgcgcttctt ccagtttgta ccaggggaac actggcattc ttttgaaggc tatgcagaga
    10861 atcaatactt tgtcgatcct tgcaaactgt tgctgacaac cccaggtatt gatgcacgta
    10921 acggcgaata tgaagcgttc ggtgtacccg cgacgattct tgctaacttc ctgcgcgaaa
    10981 atggcgtagt gccggaaaaa tgcgatctta acgccatcct cttcctgctg actccggcag
    11041 aagatatggc caaacttcag caacttgtgc cctgctggta cgcttcgaaa aactgcttga
    11101 gtccgacgcg ccattagcag aagtgctgcc ttccatctac aaacagcatg aagagcgcta
    11161 cgccggttat accctgcgtc agttgtgcca ggaaatgcat gatttgtatg cccgccacaa
    11221 cgtgaaacaa ctgcaaaaag agatgttccg caaagcgcac ttcccacacg tcagcatgaa
    11281 tccgcaagaa gccaactacg cctatttacg cggtgaagtg gagctggttc gtctaccgga
    11341 tgccgaaggt cgtatcgctg ccgaaggggc gcttccttat cctccgggtg tgctgtgtgt
    11401 tgttccgggt gaaatctggg gtggtgctgt tttgcgttac ttcagcgctc tggaagaagg
    11461 gatcaacctg ctgccaggtt ttgcaccgga gctgcagggt gtctatatcg aagaacatga
    11521 tggtcgtaag caagtttggt gctatgtcat caagcctcgt gatgcgcaaa gcaccctgtt
    11581 gaaaggggaa aaattatgag tcaggctaaa tcgaacaaaa tgggcgtcgt tcagttaacc
    11641 atactgacga tggtcaacat gatgggctcc ggtatcatca tgctgccgac aaagcttgcc
    11701 gaagttggga caatttcaat tatctcctgg ctggtgacag ccgtaggctc aatggcactg
    11761 gcatgggcat tcgcaaaatg cggtatgttc agccgtaaat caggtggtat gggcggttat
    11821 gccgagtatg ctttcggtaa atccggtaac tttattgcga actataccta cggcgtctca
    11881 ctgctgattg ctaacgtcgc gattgctatt tcggcggttg gttacggcac cgaactgctc
    11941 ggcgcgagtt tgtcgccagt gcagattggt cttgcgacca tcggggtgct gtggatttgt
    12001 accgtggcta actttggtgg tgcgcgcatt accgggcaaa tcagtagcat taccgtgtgg
    12061 gggggcatta ttccggtggt aggcctgtgc attatcggct ggttctggtt tagcccgacg
    12121 ctttacgttg attcctggaa tccgcatcat gcaccgttct tcagtgcagt aggttcttcc
    12181 atcgctatga cgctgtgggc ttttcttggt ctggagtctg cgtgtgcgaa tactgatgta
    12241 gtggaaaacc cggaacgtaa tgtgccaatc gcggtactcg gcggtacgtt aggtgcggcg
    12301 gtgatttata tcgtctccac caacgtgatt gcagggattg tgcctaatat ggagctggca
    12361 aattcaacgg caccatttgg cctggccttc gcacagatgt ttacaccaga agtgggtaaa
    12421 gtcattatgg cgctgatggt gatgtcctgc tgcggttcgc tgcttggctg gcagttcacc
    12481 attgcccagg tgtttaaatc ttcatctgat gaaggctact tccctaaaat tttctcccgt
    12541 gtaaccaaag tggatgcacc ggtgcaggga atgttgacca ttgtgattat ccagagcggg
    12601 ttggcgctga tgacgattag cccgtcgcta aacagtcagt tcaacgtgct ggttaacctg
    12661 gccgtggtga ccaatatcat tccgtatatt ctgtcaatgg cggcattagt cattattcag
    12721 aaggtcgcta atgtgccccc ctcaaaagcg aaagttgcaa actttgttgc ttttgttggc
    12781 gcgatgtata gcttttatgc gctgtactca tccggggaag aagccatgct gtacggttcc
    12841 atcgtgacct tccttggttg gacactgtat ggtctggtat caccacgctt tgaactgaaa
    12901 aataaacacg gttaaaaaag caaagtaaaa aagggcgatc ttgcgaccgc tcttttttta
    12961 ttaaatgtgt ttacgcgttt ttcagaactt cgctaacaat ctcaaccgct tctttctcaa
    13021 tctgcttgcg atgttcttca ccgaggaagc tttcgcagta gatcttatat gcgtcttccg
    13081 tgcctgacgg acgcgcggcg aaccagccgt tgtcagtcat cactttcaga ccgccaatag
    13141 aagcaccgtt gcccggagca gcagtcaggc gcgcggtgat cgggtcacct gccagggtgc
    13201 tggcgctcac catttccgga gacagcttag acagcgccgc tttttgtgcg gaagtcgcag
    13261 ctgcctgcaa acggttgtag ctcggcgcac caaagcgttt tgccagttcg ttgtagtgtt
    13321 cctgcgggtt cttaccggtg acagcggtga tttccgccgc cagcagacac ataatgatgc
    13381 cgtctttgtc ggtggaccac ggcgtgccgt cgaaacgcag gaaggaagcc cctgcgctct
    13441 cttcgccgcc aaagccgaag ctgccgtcga acagaccatc aacaaaccat ttgaaaccta
    13501 ccggtacttc taccagctta cggcccaagt cattgaccac acggtcgatc atcgcagaag
    13561 aaaccagcgt tttaccgaca gcaacatctt tgccccactg cggacgatgc tggaacaggt
    13621 agttgatcgc caccgccagg tagtggttcg gattcatcaa acctgccgga gtgacgatac
    13681 cgtgacggtc ataatccggg tcgttagcaa acgccagatc gaacttatca cgcagtgcca
    13741 gcagacccgc catcgcacac tcggaggagc agtccatacg gatcgcaccg tctttatcga
    13801 ggtgcataaa gcggaaggtt tgatcgacct gatcgttaac gatggtcagg ttgaggttgt
    13861 aatactcgcc aatgcgcttc cagtattcga taccggaacc gcccaacgga tcaacgccca
    13921 gcgtcaggcc cgctttctga atcgccgcca tatcaacgat atcggccagc ccttccacga
    13981 acggctgcac cagatcctgc tctttcacat agccggatgc catcgcttcg tcgagggaga
    14041 tacgcttcac gcctttcagg ccatcggcca tcagtgcgtt ggccttgtct tccaccactt
    14101 tggtgacgtt agtatcagcc gggccaccat ttggcggatt gtacttgata ccaccatctt
    14161 ccggcgggtt atgggacggt gtaatcacga taccgtctgc cagcgggcca ccttttttat
    14221 tgtgaaccag gatggcattg gaaacggcag gcgtcggagt gaagccattg ttttcctgca
    14281 caatgacatc aacgccgttc gctgccagca cttccagtac ggaaatgaac gcaggctcgg
    14341 acagggcgtg agtatcttta cccacatagc aagggccagt gatgccgttt ttcgcacgtt
    14401 cttcagcaat tgcctgagcg atcgccagaa tgtgcggctc gttaaagctg tggcgcgctg
    14461 cactgccacg gtgaccggaa gtaccgaatt tcaccgcgtg ctccgcattc cctgcttctg
    14521 gtttcagtac ataatattgc gccgtcagtt gggcgacgtt aatcaaatca ctctgttgtg
    14581 caggttggcc tgcacgattg tggattgcca ttgctttgtc ctttgtctgc aacgttttag
    14641 atagttccgc aaaccttctc aatcaattcc gccgggaatt gcatcgactg catgatgtgt
    14701 tcgatcatgc tgcatttacg gccggtgttg gtgttggtga tcacccaata cggcgtgcct
    14761 ggcacatgtt tcggcttggt ctgattacca tttttcagca gcgtttgttc atctgccgca
    14821 aagtacacgc gtgtacgacc gtgcaacgat tccgttgctt cggcaaacgc ctgggcgtca
    14881 agagaatata gtgtagacaa cagcagcata aagcgattga ccgctcgctt ttgctctgcg
    14941 tattcgtccg aaagcagaag ttcacgcatt gcgcgaacct tgtctttaat cgttttgacc
    15001 ggcttcgctt cgacgatagc aggtgacgca acgcgaacct ctttcgtcac cggagcagca
    15061 ggctgtgatg cggcggaaaa tttcaacata cgccgtaaaa tgtcggatgc gctctcgccg
    15121 atatgcttag tgtggctggc aatatagctg tagagttcat catcaacttc aatcgttttc
    15181 atcttaatcc agtgcagtgt cttagctgaa tacaagtcgc caagagtata tgaataaatc
    15241 ccggcagcgg atagcgtcaa gcctggcttg cagaaaaact cggaataaac cttatttgtt
    15301 gcagccctgt ggcacaatgg tcaacataat accctaaccc gacaacgcga aaaaaagaac
    15361 tttgccatga aattaaatat ccgcgcgcaa actgcacaaa accagcacaa taattctccc
    15421 atcgttcttg tccatggtct gtttggtagc ctcgacaacc ttggcgtact ggctcgcgat
    15481 ctggtaaacg atcacaatat catccaggtt gatatgcgta accacggtct ttcaccgaga
    15541 gagccggtaa tgaattaccc ggcgatggcg caggatcttg ttgataccct ggatgcactg
    15601 cagatcgaca aagcaacatt tatcggtcac tccatgggcg gtaaagcggt aatggcactt
    15661 actgcactgg cccccgatcg catcgataaa ctggtggcga tcgatatcgc accggtcgac
    15721 tatcacgtac gccgtcatga tgagattttc gcagctatca acgcggtcag tgaatcggac
    15781 gcacaaactc gccagcaagc agcagcaata atgcgccagc atcttaatga agaaggggtg
    15841 attcagtttc tgttgaaatc ttttgttgac ggggagtggc gctttaacgt gccggtattg
    15901 tgggatcagt atccgcatat tgtgggttgg gagaaaatcc cggcatggga tcaccctgcc
    15961 ctgtttatcc ctggcggcaa ttctccgtat gttagcgagc agtaccgtga tgatttactg
    16021 gcacaatttc cacaggcacg ggcgcatgtg attgcaggcg cgggtcactg ggtccatgct
    16081 gaaaaaccgg atgcggtatt acgcgctatc cgtcgctatc tcaatgatta acgcaactga
    16141 ttaaaaaccc agcgtccgcg tcgctttccg cgcgtgggcg ttggcgtgac tcatcgcgct
    16201 gatgtattat ggcgcgctat ccatccgggc agaggcctgg ctgattgttt cccccgaagt
    16261 caccaagatc atggccaaag aacaaacgga ccgtacgaca ttagatctgt tcgcgcacga
    16321 gcgtcgaccg ggacgaccga aaactaatcc gctttcgcgc gatgaacagc tgcgtattaa
    16381 taaacgcaac cagctaaaac gcgacaaagt acgtggcctt aagcgtgtcg aactgaagct
    16441 gaacgcggaa gctgtcgagg cgctgaacga gctggcggaa tcgcgtaata tgagccgtag
    16501 cgaactgatc gaagagatgt taatgcagca actggcggcg ctgcgtagtc agggcatcgt
    16561 ttaaatttcc actttcatgt agcacagtgt gcagtcctgc tcgtttgctg attccgcaag
    16621 actgcctgtt ctgctatgat tgcctttatc cgtgggcaat tttccacccc catttcaata
    16681 agtttcaaga ggttatttca ctcatggcta tcactggcat ctttttcggc agcgacaccg
    16741 gtaataccga aaatatcgca aaaatgattc aaaaacagct tggtaaagac gttgccgatg
    16801 tccatgacat tgcaaaaagc agcaaagaag atctggaagc ttacgacatt ctgctgctgg
    16861 gcatcccaac ctggtattac ggcgaagcgc agtgtgactg ggatgacttc ttcccgactc
    16921 tcgaagagat tgatttcaat ggtaaactgg ttgcgctgtt tggttgtggt gaccaggaag
    16981 attacgccga atatttctgc gacgcattgg gcaccatccg cgacatcatt gaaccgcgcg
    17041 gtgcaaccat cgttggtcac tggccaactg cgggctatca tttcgaagca tcaaaaggtc
    17101 tggcagatga cgaccacttt gtcggtctgg ctatcgacga agaccgtcag ccggaactga
    17161 ccgctgaacg tgtagaaaaa tgggttaaac agatttctga agagttgcat ctcgacgaaa
    17221 ttcttaatgc ctgatgtgat gcggcgtaga ctcatgtcta cgccgtatta atagataatg
    17281 ccaatcaaaa taattgctac aaatttgtaa cttttgctgt tgtacctata caatgtccca
    17341 gtgttcaagt ggccttgccg ttgtaaatgt aagctgtgcc acgtttttat taacaatatt
    17401 tgccagggac ttgtggtttt catttaggcg tggcaattct ataatgatac gcattatctc
    17461 aagagcaaat tctgtcactt cttctaatga agtgaaccgc ttagtaacag gacagattcc
    17521 gcatgactga taacaatacc gccctaaaga aagctggcct gaaagtaacg cttcctcgtt
    17581 taaaaatcct ggaagttctt caggagccgg acaaccatca cgtcagtgcg gaagatttat
    17641 acaaacgtct gatcgatatg ggtgaagaaa ttggtctggc tacggtatat cgcgtactga
    17701 accagtttga cgacgctggt atcgtcaccc gccacaattt tgaaggcggt aaatccgtat
    17761 ttgaactgac acagcaacat caccacgatc acctgatttg cctcgactgc ggcaaggtta
    17821 tcgaatttag tgatgattcc atcgaagcgc gtcagcgtga aattgccgca aaacatggca
    17881 ttcgcctgac taaccacagt ctctatcttt acggtcactg tgccgaaggc gattgccgcg
    17941 aagatgagca cgcgcacgaa ggcaaataag ccagcctgaa cgagaaaagc caacctgcgg
    18001 gttggctttt ttatacaagg gaaataagaa aagcgagagt tacagctctc acttatttgt
    18061 tattactgcg tatttcctgc cagaccttat cacaatcggc tttcaccgcc tgatcatttc
    18121 cggtttgcgt tgcacgcaga cactgctgat aatccagtac gcgtacactt tcctggtcgg
    18181 caaactgctg atgttgtttc tctttcttca gcacgtttaa cacgctctgg caggcttcaa
    18241 ttttttctgg cgaaccttgc gcggtgttga tacaggcgct gtatgcctct ttcagacggc
    18301 tgtcttcctg tggtgcggtt gattgcgcac aagctaccag ccccgatgcc atcatggcga
    18361 tgagaatcag ttttttcatt gaatgcggca ggcacaaaac ctgccgcgtc gggcatcaga
    18421 agatggtgaa tggtgcgatt accataaatt tcacgtcacg ctcatcctgg aagatgttac
    18481 cgtaaccgcc gccccagctt gggatgtcgg agtggttgtc gtattcggtg aagtgcagtt
    18541 tgaacatcgt gcctttggcg cgaccgtcct gaatggtgta gaccgcatcc aggctgtagg
    18601 cagactcttc aatagtccgg tttttgtcgt agtacgcatc cggattgctc tgccaggtcg
    18661 caggtttagc atcccatgcg taaacgtagg aagcgccgat ggcgaagcct ggaagattcc
    18721 agtttttcag gtcatacatc gcactgaaga agaccgcttt ttcgccgtta gcgttgaagt
    18781 cggaacggtt atcccaccag atatccaggc ggccgttgga ggaagcgtag gttggggtca
    18841 tacgttgcag gaagtatccc tgctgactgt cagccttaat ccaggtgcct tcgaggcgca
    18901 aatccactac gtcagccgcc cggtaaccaa aggtcaacgc ttgcagccag gcggtgccgt
    18961 cataaagatc gttgacgctg cgatcgtcaa ctttatcgcg ggtaccatag aactggtagc
    19021 tggtggttaa cgggctaccg gcgatatcaa atttgtagct ggctttggca aaatattgat
    19081 cgatataccc ttccgcctga ccaaatgccg cttccagtac gaagttattt ttgaagtcgt
    19141 atttcgcacc gatggagtgc agataatcaa ctttggtggt tttatcgttc tgataaaact
    19201 catccatttc cagatgccac ggcgctttgt attcgttggt ccacatgtag gagaaactca
    19261 aggcaccagc atcgccgtaa tcaaaattcg ccccggcttc cgccccctga taagtacccg
    19321 gcataaagct ccagtgcggc gctaacagcg tttgaccggt tggctgaatg taacctgccc
    19381 tcgcccaaac cggaccgtat ttaaatttgg ccgcagcttt atacaggctt ataccgcttt
    19441 tgtcgccgga ccagtcttcg tcataggctt tattactttt tgaaaacgcg atttcgttcg
    19501 ggtgggagct gtcgccgttt tccgccattt caatcgccgt aaacgcagca atatcaaggc
    19561 cgaacatatc agcagcataa ccggactgaa aatcgaggtt ggcattccag gtggagtgag
    19621 aaaggttggt tttgtatttg tcgccgtcgg taacatcttt acggtcgcgt tcacgctgcc
    19681 agtaatagat accgccggtt aaggttgaat cgtcgatgaa tccttcggcg cgagcctccg
    19741 gcattgccat aaagcccgac attgctgtaa caccggcaat agccagcgcc agcgtactac
    19801 gtttgccact aaacgtacgc atgggttaat cctctttgac gtataaattg ctgcaccaaa
    19861 ggtgaacagc gaaaaaaaat aaaaattaaa aaagtagcgt tgagaaaagt ctcgctgact
    19921 acaggaacag actatttttc gcgacgcgaa ttatcaatct ctttctgtaa ccagaatgta
    19981 agaacatgag caaccgcaca tattttgatg atttttgtta caaggcttaa gtttgcggtt
    20041 gaaaggcttg cagaagaata aaaacgtgaa attccagcaa gatgaaagcg gttacatttt
    20101 atttaattat gttttgtgat tttaaattta ttttatggat aaattgcgaa gcggaattaa
    20161 ttcgcgaaga aaagcagaaa aaacgccgca gagcgcggcg tttgggattg tttgctattt
    20221 cttgccggat gcggcgtgaa cgccttatcc ggcctacaaa agcacgcaaa ttcaatatat
    20281 tgcagagatc atgtaggcct gataagcgaa gcgcatcagg caatttagcg tttgaaattt
    20341 attgtctatt tattgccgga tgcgacgtaa atgcctcacc cggcttggta gaacttaaaa
    20401 ttactcgcct actttcgccc aggtatcacg cagcccaacg gtgcggttaa ataccggttt
    20461 ttcggccgtg gaatggcggc tgtcgaggca gaagtaacct tcacgctcaa actggaatgc
    20521 tttacccgca accgcatctt tcagcgacgg ttcagcaaag ccctgtttga tcaccagcga
    20581 ttccgggtta atcaccgaca ggaaatcatc cgcagcacct gggttaggca cgctgaacag
    20641 acgatcatac aaacggattt caaccggcag cgcatgtgcc gcgctcaccc agtgaataac
    20701 acctttgact ttacgaccat ctgccggatc tttgcttaag gtgtcggcgt cataagtaca
    20761 gaagatggtg gtgatattac cttcggcatc tttctccacg cgttcagcct taatgacgta
    20821 agcattacgc agacgcactt ctttacccag caccagacgt ttgtactgct tgttagcttc
    20881 ttcgcggaaa tcggcgcgat cgatccaaat ttcaccgcta aacggcacct gacggctccc
    20941 catttccggt ttgttcggat ggttcggcat ggtgaccatt tcgccttcgc cctgatagtt
    21001 ttcgataacc agtttcaccg gatcgataac cgccattgcg cgcggcgcat tttcgttgag
    21061 atcttcacgg atgcaggatt ccagcgacgc catctcaatg gtgttgtcct gcttggtcac
    21121 gccgatgcgt ttgcagaact cacgaataga agccgcagtg taaccacgac gacgcagacc
    21181 ggaaatggtc ggcatacgcg ggtcatccca gccttcaacg tgcttgtcgg tcaccagcag
    21241 gttcagctta cgcttggaca tcacagtgta ttccagattc aggcgcgaga attcgtactg
    21301 gcgcggatga acaggaatgg tgatgttatc cagtacccag tcgtacagac gacggttgtc
    21361 ctggaactca agcgtacaca gagagtgcgt aataccttcc agcgcatcgc tgatgcaatg
    21421 ggtgaagtcg tacatcgggt agatgcacca cttgttgcca gtctggtggt gttcagcaaa
    21481 tttaatgcgg tacagcaccg gatcgcgcat tacgataaat ggcgacgcca tgtcgatttt
    21541 cgcacgcagg caggctttac cttcttcaaa accaccggca cgcatttttt cgaacagcgc
    21601 caagttctct tcaacgctgc ggtcgcggta cgggctgttt ttacccggct gcgtcagggt
    21661 accgcggtat tcgcggatct gttccggtgt cagttcatca acgtacgcca ggcctttatt
    21721 gatcagttcg atcgcatagg cgtggagctg atcaaaataa tcggaggagt aacggacgtt
    21781 accagaccag tgaaaaccta accactctac gtcgtttttg atcgaatcga catactcgat
    21841 atcttctttt accgggttag tgtcgtcgaa acgcaggttg cactggcctt tatagtcctg
    21901 ggcgatcccg aagttcaggc agatagattt cgcatggcca atatgcagat agccattcgg
    21961 ctccggcggg aaacgggtgt gtactgtgat gtgcttacca ctggccagat cttcatcgat
    22021 gatctgacgg ataaagttag tcgggcgggc ttctgcctca ctcatcgtgg attcctcaaa
    22081 gcgtaaacaa cgtataacgg cgtatgatct tataagcggg acaggctgac aactgttagt
    22141 tttttaaaat tgataatcta acaaggatta tggggatgat tgatgaaaaa aaaagcggca
    22201 gagaagatct ccaccgcttg tcattgttgg atgcgacgct gccgtgtcgc atcaggcata
    22261 aagcagatta ctttttgatt tcatacagcg gtgtttgacc cgccacaaca tggccctgag
    22321 ctttaatgag caagccactg aaatcgtcga tattgctgca aaccaccggg ctaatcatcg
    22381 agcgggcgtt agcgttcagg taatccagat ccatttccag aatcggttgc cctgcgctta
    22441 cctgcgcacc ctcttccacc agacgtttaa agcctttacc ttccagcgct acggtgtcga
    22501 tacccatatg gacgacgatc tccgcgcctt tttcggtttc caggcagaac gcgtggttgg
    22561 tgttgaagat tttcacgatt gtccctgcgg ctggtgatac gacgatttta tctgtcggtt
    22621 tcaccgccac accgtcaccc accgctttgc tggcgaatgc ttcgtcagga acctgatcca
    22681 gtgccacgac atcaccggta atcggcgata ccagctccgc gatagatacc gcgtttggta
    22741 cagcctgcgg ttttgctaca ggcgcggcag ttgccggagt tgcttcagct gacgcagcgg
    22801 ctaccggacc acgggcaacg actttcttca tcgcatcgcc gatggattct gctttcgcgc
    22861 caacaatcac ctgaatagtc tgtttgttca gtttcactac accagaagca cccagacgtt
    22921 tacacatcgt atcgttgacg cgggcagagt cagccacggt caggcgcaga cgggtaatac
    22981 aggcatcaat cgctttcagg ttgtcagtac cgccaaccgc agcaatatag ttggttgcca
    23041 gttgagtcag accttcttca gtattgctgt tggcttcttc agtaacgatc tcgtcttctt
    23101 tatcttcacg acccggcgtt ttcaggttga acatgcggat aaccaaactg aacaccacga
    23161 agtagatagc gaagaagata acgcccatca ccagcagcat ccagacgttc tggctggcgg
    23221 caggcaggtt atacatcaac gcgtagtcga tagcacccgc agagaaagag aagccggcat
    23281 ggatacccag cagcgttgcc acaaacaggc tgataccggt cagcagtgcg tgcaggaggt
    23341 acagcagcgg cgcaaggaac atgaacagga attccagcgg ctcagtcaca ccggtcagga
    23401 acgcagtaac agcaacagaa agcagcatcc cgccaaccat cggacgacgc tctttcggtg
    23461 ctgcgaagta catcgccagc gccgcacccg gcagaccgaa catcatgatc gggaagaagc
    23521 cggacatgaa catccccgcg gtgccgtcac cagcgtagaa acggttgatg tcgccgtgga
    23581 aaaccgtacc cgccgcgttg gtgaattcac caatctggaa ccaggcgatg gtgttcagca
    23641 cctgatgcag accggttggg atcagcagac ggttgatgaa accaaagata ccggaaccca
    23701 gcgcgcccgc agaaacgatc cactcgccgc ctgcatggat agcgtgctgt accggcggcc
    23761 agacgtaacc aaaaatggcc gccagcacca gacagaagaa gccggtggcg atcggcacaa
    23821 agcgtttgcc gccgaagaag ctcaggaagt ccggcagttt aatatcggac caacggttat
    23881 aggctgcgcc accaaccaga ccggtaatga tacccgccag tacacccatg ttaatttctg
    23941 ggttgatggt caccatcgct ttggttaaca caaagtaacc taccgcaccc gccagcgccg
    24001 ccgcacctgc gctgtctttc gaccagctgg atgccacacc gatggcgaag attaatgcga
    24061 ggttatcaaa aattgcaccg cccgcctggg caataaacgc aacgttaagt aaatctggct
    24121 gaccgaatcg cagcaacagc gccgccaccg gcagcaccgc gatagggagc tgtaacgccc
    24181 taccgagtcg ctggaaaaaa cctaaaatat tcatcttatt ccccctacga gaaccctatt
    24241 tggctcgttt aaagccgtat ttttattttg ctgcaaattt tactgccgat gttctgtaat
    24301 cagattgtta gatcatctgc tacagagtgt gtgaaaattt aattcgcatc gcaaattaaa
    24361 cgcgtgtctt ttgtgagttt tgtcaccaaa tatcgttatc atcactccct tttactggct
    24421 aaaccagaaa acttatttta tcattcaaaa aatcaagtcg gattgacgcc tgtctgcgca
    24481 aatccaggtt acgcttaaag atgtctaatc cgccaacggc ttacatttta cttattgagg
    24541 tgaataatga gactgatccc cctgactacc gctgaacagg tcggcaaatg ggctgctcgc
    24601 catatcgtca atcgtatcaa tgcgttcaaa ccaactgccg atcgtccgtt tgtactgggc
    24661 ctgccgactg gcggcacgcc gatgaccacc tataaagcgt tagtcgaaat gcataaagca
    24721 ggccaggtca gctttaagca cgttgtcacc ttcaacatgg acgaatatgt cggtctgccg
    24781 aaagagcatc cggaaagcta ctacagcttt atgcaccgta atttcttcga tcacgttgat
    24841 attccagcag aaaacatcaa ccttctcaac ggcaatgccc cggatatcga tgccgagtgc
    24901 cgccagtatg aagaaaaaat ccgttcttac ggaaaaattc atctgtttat gggcggtgta
    24961 ggtaacgacg gtcatattgc atttaacgaa ccggcgtctt ctctggcttc tcgtactcgt
    25021 atcaaaaccc tgactcatga cactcgcgtc gcgaactctc gtttctttga taacgatgtc
    25081 aatcaggtgc caaaatatgc cctgactgtc ggtgttggta cactgctgga tgccgaagaa
    25141 gtgatgatta tggtgctggg tagccagaaa gcactggcgc tgcaggccgc cgttgaaggt
    25201 tgcgtgaacc atatgtggac catcagctgt ctgcaactgc atccgaaagc gatcatggtg
    25261 tgcgatgaac cttccaccat ggagctgaaa gttaagactt taagatattt caatgaatta
    25321 gaagcagaaa atatcaaagg tctgtaattg ttatccctgc cctggttcct tgctcagggc
    25381 aatatttttt aaaatcgggg ggcagaatgt atgcattaac ccagggccgg atctttaccg
    25441 gccacgaatt tcttgatgac cacgcggttg ttatcgctga tggcctgatt aaaagcgtct
    25501 gtccggtagc ggaactgccg ccagagatcg aacaacgttc actgaacggg gccattctct
    25561 cccccggttt tatcgatgtg cagttaaacg gctgcggcgg cgtacagttt aacgacaccg
    25621 ctgaagcggt cagcgtggaa acgctggaaa tcatgcagaa agccaatgag aaatcaggct
    25681 gtactaacta tctgccgacg cttatcacca ccagcgatga gctgatgaaa cagggcgtgc
    25741 gcgttatgcg tgagtacctg gcaaaacatc cgaatcaggc gttaggtctg catctggaag
    25801 gtccgtggct gaatctggta aaaaaaggca cccataatcc gaattttgtg cgtaagcctg
    25861 atgccgcgct ggtcgatttc ctgtgtgaaa acgccgacgt cattaccaaa gtgaccctgg
    25921 caccggaaat ggttcctgcg gaagtcatca gcaaactggc aaatgccggg attgtggttt
    25981 ctgccggtca ctccaacgcg acgttgaaag aagcaaaagc cggtttccgc gcggggatta
    26041 cctttgccac ccatctgtac aacgcgatgc cgtatattac cggtcgtgaa ccgggcctgg
    26101 cgggcgcgat cctcgacgaa gctgacattt attgcggtat tatcgctgat ggcctgcatg
    26161 ttgattacgc caacattcgt aacgctaaac gccttaaagg cgacaaactg tgtctggtta
    26221 ccgacgccac cgcgccagca ggtgccaaca ttgaacagtt catttttgcg ggtaaaacaa
    26281 tatactaccg taacggactt tgtgtggatg agaacggtac gttaagcggt tcatccttaa
    26341 ccatgattga aggcgtgcgt aatctggtcg aacattgcgg tatcgcactg gatgaagtgc
    26401 tgcgtatggc gacgctctat ccggcgcgtg cgattgacgt tgaaaaacgt ctcggcacgc
    26461 tcgccgcagg taaagtagcc aacctgaccg cattcacacc tgattttaaa atcaccaaga
    26521 ccatcgttaa cggtaacgag gtcgtaactc aataagagaa agtatgacac caggcggaca
    26581 agctcagata ggtaatgttg atctcgtaaa acagcttaac agcgcggcgg tttatcgcct
    26641 gattgaccag tacgggccaa tctcgcggat tcagattgcc gagcaaagcc agcttgcccc
    26701 cgccagcgta accaaaatta cgcgtcagct tatcgaacgc gggctgatca aagaagttga
    26761 tcagcaggcc tccaccgggg gccgccgcgc tatctccatc gtcaccgaaa cccgcaattt
    26821 ccacgcaatc ggcgtacggc ttggtcgtca tgacgccacc atcactctgt ttgatctcag
    26881 cagcaaagtg ctggcagaag aacattaccc gctgccggaa cgtacccagc agacgctgga
    26941 acatgccctg ctgaatgcca ttgctcagtt tattgatagc taccagcgca agctgcgcga
    27001 gctaatcgcc atttcggtga tcctgccagg gcttgttgac ccggacagcg gcaaaattca
    27061 ttacatgccg catattcagg tagaaaactg ggggctggta gaagcactgg aagaacgttt
    27121 taaagtgacc tgtttcgttg gtcacgatat ccgtagtctg gcgctggcgg agcactactt
    27181 cggtgcaagt caggattgcg aagactccat tctggtgcgt gtccatcgcg gaaccggggc
    27241 cgggattatc tctaacgggc gcatttttat tggccgcaac ggtaacgtcg gtgaaattgg
    27301 ccatattcag gtcgaaccgc tgggtgaacg ctgccactgc ggcaactttg gctgcctgga
    27361 aactatcgcc gccaacgctg ccattgaaca acgggtgttg aatctgttaa agcagggcta
    27421 ccagagccgt gtgccactgg acgactgcac catcaaaact atctgcaaag ccgcgaacaa
    27481 aggcgatagc ctggcctcgg aagtgattga gtatgtcggt cgtcatctgg gtaaaaccat
    27541 cgccattgct atcaacttat ttaatccgca aaaaattgtt attgctggtg aaatcaccga
    27601 agccgataaa gtgctgctcc ctgctattga aagctgcatt aatacccaag cgctgaaggc
    27661 gtttcgtact aatctgccgg tggtacgttc tgagctggat caccgctcgg caatcggcgc
    27721 ttttgcgctg gtaaaacgcg ccatgctcaa cggtattttg ctccagcatt tgctggaaaa
    27781 ttaatgtgct tttatagtgg cgcttattgt tgtcaatatt ctgggtagtc catgaccatt
    27841 aaaaatgtaa tttgcgatat cgacggcgtg ctgatgcacg ataacgtcgc cgtaccgggt
    27901 gcagcggaat ttttgcacgg gattatggat aaaggcctgc cgctggtgtt gctgaccaac
    27961 tatccttcgc agactgggca agatctggcg aaccgctttg ccactgccgg tgtcgatgtg
    28021 ccggatagcg tgttttatac ctctgcgatg gcgaccgccg atttcctgcg tcgctaggaa
    28081 ggcaagaaag cgtatgtggt gggcgaaggc gcactgattc atgaactgta caaagccggt
    28141 ttcactatta ccgatgtgaa ccctgatttc gtgattgttg gcgaaacacg ttcctacaac
    28201 tgggacatga ttcataaagc agcctatttc gtcgctaacg gtgcacgctt tatcgccacc
    28261 aacccggaca cccacgggcg cggtttttat cccgcttgtg gcgcgttgtg tgcagggatt
    28321 gagaaaatct ccgggcgcaa accgttctat gttggtaagc ccagcccgtg gatcatccgc
    28381 gcggcattaa acaaaatgca ggcgcattcg gaagaaacag tgattgtcgg cgataacctg
    28441 cgtaccgata ttctggcggg cttccaggcc ggtctggaga cgattctggt gctttctggt
    28501 gtttcatcgc tcgacgatat cgacagtatg ccgttccgcc caagctggat ttacccgtca
    28561 gtcgctgaaa tcgacgttat ctgaatacca cccacgtctg cggacgtggt tggctctgat
    28621 gacaaacgcc aaactgcctg atgcgctacg cttatcaggc ctacgcagct cctgcaatat
    28681 attgaatttt catgcttttg taggccggat aaggcgttca cgccgcatcc ggcatgaaca
    28741 aatcgcactt tatcagcaat ttggcccccg taaaatataa ccattcaata aaacccgcta
    28801 aaaattatcg attcatcaat aaatacatcc tcacaccaga ccctttttca ccaatcgaca
    28861 atcaccatta cgttttttat ttttgcactg ccaaatagct tgcgcacagt gctgctttgc
    28921 ggcatttttt taaacaagca aacacaacaa gcaacaaata ccaggttaac ggagaaggtt
    28981 atgtgttcaa tttttggcgt attcgatatc aaaacagacg cagttgagct gcgtaagaaa
    29041 gcactcgagc tgtcacgcct gatgcgtcat cgtggcccgg actggtccgg tatttatgcc
    29101 agcgataacg ccattctcgc tcacgaacgt ctgtcaattg ttgacgttaa cgcgggggcg
    29161 caacctctct acaaccaaca aaaaaaccac gtactggcgg taaacggtga aatctacaac
    29221 caccaggcat tgcgcgctga atatggcgat cgttatcagt tccagacagg gtctgactgt
    29281 gaagtgatcc tcgcgctgta tcaggaaaaa gggccggaat ttctcgacga cttgcagggc
    29341 atgtttgcct ttgccctgta cgacagcgaa aaagatgcct acctgattgg tcgcgaccat
    29401 ctggggatca tcccactgta tatggggtat gacgaacacg gtcagctgta tgtggcctca
    29461 gaaatgaaat cgctggtgcc agtttgccgc acgattaaag agttcccggc ggggagctat
    29521 ttgtggagtc aggacggcga aatccgttct tattatcatc gcgactggtt cgactttgat
    29581 gcagtgaaag ataacgtgac cgacaaaaac gagctgcgtc aggcactgga agattccgtt
    29641 aaaagccatc tgatgtctga tgtgccttac ggtgtgctac tttctggtgg tctggattcc
    29701 tcaattattt ccgctatcac caagaaatac gcagcccgtc gcgtggaaga tcaggaacgc
    29761 tctgaagcct ggtggccgca gttacactcc tttgctgtag gtctgccggg ttcaccggat
    29821 cttaaagcag cccaggaagt ggcaaaccat ctgggcacgg tgcatcacga aattcacttc
    29881 actgtacagg aaggtctgga tgccatccgc gacgtgattt accacatcga aacttatgat
    29941 gtgaccacta ttcgcgcttc aacaccgatg tatttaatgt cgcgtaagat caaggcgatg
    30001 ggcattaaaa tggtgctgtc cggtgaaggt tctgatgaag tgtttggcgg ttatctttac
    30061 ttccataaag cgcccaacgt taaagaactg catgaagaga cggtgcgtaa actgctggcc
    30121 ttgcatatgt atgactgcgc gcgcgccaac aaagcgatgt cagcctgggg cgtggaagca
    30181 cgcgttccgt tcctcgacaa aaaattcctt gatgtggcga tgcgcattaa cccgcaggat
    30241 aaaatgtgcg gtaacggcaa aatggaaaaa cacatcctgc gtgaatgttt tgagtcatac
    30301 ctgcccgcaa gcgtggcctg gcggcagaaa gagcagttct ccgatggcgt cggttacagt
    30361 tggatcgaca ccctgaaaga agtggcggcg cagcaggttt ctgatcagca actggaaact
    30421 gcccgcttcc gcttcccgta caacacgccg acctcaaaag aagcgtatct gtaccgcgag
    30481 atctttgaag aactgttccc acttccgagt gccgctgagt gcgtgccggg cggtccttcc
    30541 gtcgcgtgtt cttcagctaa agcgatcgaa tgggatgaag cgttcaaaaa aatggacgat
    30601 ccgtctggtc gtgcggttgg cgttcaccag tcggcgtata agtaagaaat gcggtactgc
    30661 cccggagaaa tccggggctg ttttagttat tgcgctgctt gttgattctg atatgcagta
    30721 aggaaatgtt ccgggtttgc cacatcactg tttgacaact catcactgta ggccagtatt
    30781 tcatagatta atttcccacc actattgttg aattccgaat ttcatcctgt acgacaaaag
    30841 aatggctgta gggtggggaa aaacacaaag taaacaataa ttgacgaata tagcgccacg
    30901 ctgttcgcaa cctaaccaaa cagtcacttt cgagcaattt tccttgaaaa agaggttgac
    30961 gctgcaaggc tctatacgca taatgcgccc cgcaacgccg ataaggtatc gcgaaaaaaa
    31021 gatggctacg tagctcagtt ggttagagca catcactcat aatgatgggg tcacaggttc
    31081 gaatcccgtc gtagccacca tcttttttgc gggagtggcg aaattggtag acgcaccaga
    31141 tttaggttct ggcgccgcaa ggtgtgcgag ttcaagtctc gcctcccgca ccattcacca
    31201 gaaagcgttg tacggatggg gtatcgccaa gcggtaaggc accggttttt gataccggca
    31261 ttccctggtt cgaatccagg taccccagcc atcgaagaaa taatctggct acgtagctca
    31321 gttggttaga gcacatcact cataatgatg gggtcacagg ttcgaatccc gtcgtagcca
    31381 ccaaattctg aatgtgtcga atacgttcgg caaattcaaa aaccaatttg ttggggtatc
    31441 gccaagcggt aaggcaccgg attctgattc cggcattccg aggttcgaat cctcgtaccc
    31501 cagccaattt attcaagacg cttacatcgt aagtgcaccc agttggggta tcgccaagcg
    31561 gtaaggcacc ggattctgat tccggcattc cgaggttcga atcctcgtac cccagccaca
    31621 ttaaaaaagc tcgcttcggc gagctttttg cttttctgcg ttcattcaat gtcgaatgcg
    31681 atgttgacac gtcttatcct tcaatgtcgg atgcgacgct gccgcgtctt atccgaccta
    31741 cggctggcac gcatccggca atgttgtaag gctacaaccc taacgcatat ttcagcgcct
    31801 gacgtttcaa cacgccagca cgctctgccg ccattaaccc gagattacgc acaaaacgca
    31861 gcggcggcag attattactg aatccggcat aaaacagatc cataccactt tgcataatga
    31921 agttatccgc catgcgccgc atctggtaac gcttgaggac aggataactg gcccacgctt
    31981 caccgtagct gcgggcattc accagaacat caatcagggc atcgacatca cgataaccaa
    32041 gattcacccc ctgccccgcc agcggatgga tggtatgcgc ggcatcgccc accagcgcaa
    32101 gccccggctg cacatactgc aacgcatgtc ggcgcgtcag cggaaacgca ccagcggcaa
    32161 gcggtgtaac gtaacccaga cgcgacggga aatgcttcgc gatttccgcc tggagttgcg
    32221 ccatattcat attctgcaac tggcgaatac gcgtcggaga gtcataccac accagcgatg
    32281 cccagttatc aaacaacggc agaaacgcac gcggtccgtc cggggtaaat tgctgccagg
    32341 tgctgtcgcc gggatcgttc tcacactgta cgctaatcaa catgcacgac tgcgcatact
    32401 gccaggcatg aacgccaatt cccgccatct gccgcacctg agaatttgcg ccgtcggcac
    32461 caatcaccag tttcgcgcgg atcgtttcac cgcccttcag ttccagctcc tgaagatcat
    32521 tatctcgatg taatgcaatc agcgaggttg gcacacgtaa cgttactttc ggatgcgctt
    32581 ccagcgcctg ccacaacgcc tgttgcagga ctgtgttttc caccatgtag ccaagcaacg
    32641 gcaactttaa ttcggctgcg tcaaatacca catgtgccgt ttcccactcc caggtttcca
    32701 gtctgcggta aggatggcaa cgcatagcct gtactgcatc ccagacccct aaccctttaa
    32761 gcaatgatac cgaagccgcg ctgatcgccg agatccgcac gtccggttgg ctgtcagcga
    32821 caaacggtgc aggttctgcg tgctcgatca ccgttaccga aaatccgtgc tgtgccagcc
    32881 ccagcgccag tgcgccgccg accattcctc cgccgacaat ggcaatttcc gttggttgat
    32941 ttgtcatggt caatcatcct gttagcaata tgctttaagt ttacaggatt tttaccccca
    33001 agggctgata acgctcatac tggtcacaac ggcagcaaag cattacacta tgcgccctgc
    33061 attcctggct actatttcgc aagagcaagt cgatgaccaa aaaactccat attaaaacct
    33121 ggggctgtca gatgaacgag tacgattcat cgaagatggc cgatctgctg gatgccaccc
    33181 acggctatca actgaccgac gtggcggaag aagcggatgt gctactgctg aacacctgct
    33241 caatccgcga gaaggctcag gaaaaagtct tccatcagtt gggtcgctgg aaactgttaa
    33301 aagagaagaa tccagacctg attatcggcg tcggtggctg cgtggcatcg caagaaggcg
    33361 agcacattcg ccagcgcgcc cactatgtcg atattatttt tgggccgcaa acgctgcacc
    33421 gtctgccgga gatgatcaac tccgtgcgcg gcgaccgcag cccggttgta gatatcagct
    33481 tcccggaaat cgagaagttt gaccgtctgc cggaaccgcg cgccgaaggg ccgaccgcgt
    33541 ttgtctccat catggaaggc tgcaataaat attgcaccta ctgcgtggtg ccttacaccc
    33601 gtggtgaaga ggtaagccgt ccgtctgacg atattctgtt tgagattgcc cagcttgcgg
    33661 ctcagggcgt gcgtgaagtc aacctgctcg gtcagaatgt gaatgcctgg cgtggtgaga
    33721 actacgacgg caccaccgga tcgtttgccg atctgttgcg tctggttgct gcgatcgacg
    33781 ggatcgatcg tattcgcttt accaccagcc atccgatcga attcaccgac gatatcatcg
    33841 aagtgtaccg cgacacgcct gagctggtga gcttcctgca tctgccggta cagagcggtt
    33901 ccgatcgcat tctgaacctg atgggacgta cccatacggc gctggagtac aaagcgatca
    33961 tccgtaaact gcgtgcggcg cgtccggata ttcagatcag ttctgacttc attgttggct
    34021 tccctggcga aaccaccgaa gacttcgaga aaacgatgaa gctgattgcc gacgtcaatt
    34081 tcgacatgag ctacagcttt atcttctctg cacgtccagg aacgcctgcc gccgatatgg
    34141 ttgatgatgt tccggaagaa gagaagaagc agcgtctgta tattctgcaa gaacgtatca
    34201 atcagcaagc gatggcatgg agccgtcgga tgctcggcac cacccagcgt attctggtag
    34261 aagggacttc gcgtaagagc atcatggagc tttccgggcg taccgaaaat aaccgcgtgg
    34321 tcaacttcga gggcacaccg gatatgatcg gtaaattcgt cgatgtagaa attaccgacg
    34381 tctacccgaa ctctctgcgc ggtaaagtgg tgcgaactga agatgaaatg ggtctgcgca
    34441 tggcagaaac accggagtcg gtgattgccc gcacccgcaa agaaaacgac cttggcgtgg
    34501 gttattatca gccgtaattc tcaggcctgc cgttccggtg ggccttgttt tttctctctt
    34561 tatccccatc tttatcgcaa tgcttgcgcc tttttcctgt ggcgtgaata atttccttat
    34621 aggccggtga atgttcagct tccggcggca tacagataag aggaacggtt tgaacataga
    34681 cactcgcgaa atcaccctgg agccagcaga caatgcgcgt ctgttgagcc tgtgcggccc
    34741 gtttgatgac aacatcaagc agctcgaacg ccgtctcggc atcgagatca atcgccgcga
    34801 taaccacttt aaactgaccg gccgtccgat ttgcgtcacc gctgcggcag acattctgcg
    34861 tagcctgtat gtcgatactg ccccgatgcg cggtcagatt caggatatcg aaccggaaca
    34921 gatccacctt gcgattaaag aagcgcgggt actggagcaa agcgcggaga gcgtgccgga
    34981 gtacggcaaa gcggtcaata tcaaaactaa acgcggcgta attaagccgc gcacgccaaa
    35041 ccaggcgcag tacatcgcca atattctcga ccatgacatt accttcggcg ttggcccggc
    35101 gggtacgggt aaaacctacc tggcagtggc tgcggcagtt gatgccctgg agcgtcagga
    35161 aattcgccgt attctgctga ctcgtccggc ggtcgaagcc ggtgagaaac tgggcttcct
    35221 gcctggcgat ttaagccaga aagtagaccc gtatctgcgc ccactgtacg acgcgctgtt
    35281 tgaaatgctg ggctttgaga aagtcgagaa actgattgag cgcaacgtta ttgaagtcgc
    35341 gccgctggcc tatatgcgtg gtcgtacgct gaacgacgcg tttatcattc tcgatgagag
    35401 ccagaacact accatcgaac agatgaagat gttcctgacc cgtatcggtt ttaactcaaa
    35461 agcggttatc accggcgacg tcacacagat cgacctgccg cgtaatacta aatcaggctt
    35521 acgtcatgcc attgaagtgc tggccgatgt cgaagagatc agctttaact tcttccacag
    35581 cgaagacgtg gttcgtcacc cggtggtggc gcgtatcgtt aacgcttatg aagcctggga
    35641 agaagcagaa caaaaacgta aagcggcgct ggcagcagaa cgcaagcgcg aagaacagga
    35701 acaaaaatga gtcaggtgat cctcgattta caactggcat gtgaagataa ttccgggtta
    35761 ccggaagaga gccagtttca aacatggctg aatgcggtga tcccgcagtt tcaggaagag
    35821 tcggaagtga ctattcgcgt ggtcgatacc gccgaaagcc acagtctgaa tctgacctat
    35881 cgcggtaagg ataagccgac caacgtgctc tccttcccgt ttgaagtgcc gcctggcatg
    35941 gagatgtcgc tactgggcga tctggttatc tgccgtcagg tggttgagaa ggaagctcag
    36001 gagcaaggca aaccactgga ggcgcactgg gcgcatatgg tggtgcacgg cagtctgcat
    36061 ttgttaggtt acgatcacat tgaagatgac gaagcagaag aaatggaagc cctcgaaaca
    36121 gagattatgc ttgctctggg ctatgaggat ccgtacattg ccgagaaaga ataatttgcc
    36181 aggcatttga ctggcagacg tccccgccgt tggtgattaa cgacggcgtt ttgattaact
    36241 aacatgagaa cccatacgac gccatgagcg acgacaattc acacagtagt gacacgataa
    36301 gcaacaagaa gggatttttc tccctgttac tcagccaact tttccacggt gaaccgaaaa
    36361 accgtgacga actgctggcg ctgatccgtg attccgggca gaacgacctt atcgacgaag
    36421 atacgcgcga tatgctcgaa ggggtgatgg acatcgcaga ccaacgcgtt cgcgacatca
    36481 tgatcccccg ctcccagatg attaccctga aacgcaacca gacgctggac gaatgccttg
    36541 atgtcatcat cgagtccgcc cactcacgtt tcccggtgat tagcgaagac aaagatcaca
    36601 ttgaagggat tctgatggcg aaagacttgc tgccgtttat gcgcagcgat gctgaagcct
    36661 tcagcatgga caaagtgtta cgtcaggcgg ttgtcgttcc tgaaagtaag cgcgtagacc
    36721 ggatgctgaa agagtttcgc tctcagcgtt accacatggc gatcgttatt gacgaattcg
    36781 gtggggtttc cggtctggtg accattgaag acatcctgga actgattgtt ggtgagattg
    36841 aagacgagta tgacgaagaa gatgatatcg acttccgtca gctgagtcgt catacctgga
    36901 ccgtgcgcgc actggcttcc attgaagact tcaacgaagc gttcggcacc cactttagcg
    36961 atgaagaagt cgacactatc ggtggtctgg tgatgcaggc gtttgggcat cttccggcgc
    37021 gtggcgaaac tatcgacatc gacggttacc agttcaaagt ggcgatggcc gatagtcggc
    37081 gtattattca ggttcatgtc aaaatcccgg atgactcacc ccagccaaag ctggatgaat
    37141 aaaaccgaaa ctggatagat aactacatgg attttgcctc attaattgaa cgccagcgca
    37201 ttcgcctgct gctggcgtta ttattcggtg cctgcggaac gctggccttc tctccttacg
    37261 acgtctggcc tgcggcgatt atttcgctga tggggcttca ggcgttgacc tttaaccgtc
    37321 gcccactcca gtctgccgct attggctttt gctggggatt tggcctcttt ggcagcggta
    37381 ttaactgggt ctatgtcagc atcgcgacct ttggcgggat gcctggcccg gttaacatct
    37441 tcctggtggt gctgctggcg gcgtatttgt cgctgtatac cggactgttt gctggcgtgc
    37501 tgtcgcgtct gtggccgaaa accacctggc tgcgcgtggc gattgccacc cctgctctct
    37561 ggcaagtgac cgagtttctg cgcggttggg tgctgactgg cttcccgtgg ttacagttcg
    37621 gctatagcca gattgatggc ccgttaaaag ggctggcacc gctaatgggc gtggaagcca
    37681 ttaacttcct gctgatgatg gttagtggcc tgctggcact ggcgttggtc aaacgcaact
    37741 ggcgtccgct ggtggtggcc gtcgtgctgt ttgccctacc cttcccgctg cgttacatcc
    37801 aatggtttac cccgcaaccg gagaaaacca ttcaggtttc gatggttcag ggcgatattc
    37861 cgcaatcgct gaaatgggac gaaggccagc ttcttaatac gctaaagatt tactacaacg
    37921 caacggcacc gctgatgggc aaatcatcgt tgattatctg gccggagtcg gcgataaccg
    37981 atctggaaat taatcagcaa ccgttcctca aagcactgga cggtgagttg cgtgataaag
    38041 gtagctcgct ggtaaccggg attgtcgacg cgcgtctcaa taagcagaac cgctacgata
    38101 cctacaacac catcatcacg ctgggtaaag gtgcaccgta cagctacgaa tcagccgatc
    38161 gctataacaa aaaccatctg gtgccgtttg gcgagtttgt cccgctggag tcgattctgc
    38221 gtccgttagc accgttcttt gatctgccga tgtcgtcgtt cagccgtggg ccatatatcc
    38281 agccgccgct gtcggcaaat ggtattgagc ttactgcggc tatttgctac gagattattc
    38341 tcgccgagca agtgcgcgat aacttccgcc cggataccga ctacctgctg actatctcca
    38401 acgatgcgtg gtttggtaaa tctattggtc catggcaaca cttccagatg gcgcgaatgc
    38461 gtgcgctgga gctggcgcgc ccactgttgc gcagcaccaa caacggcatt acggcggtga
    38521 ttggcccgca gggtgagatt caggcgatga tcccgcagtt cgcccgcgag gtgttaacca
    38581 ctaacgtgac gccgaccacc ggactcacac catacgcacg taccggcaac tggccgctgt
    38641 gggtgctgac ggcactgttt ggttttgctg ctgtgttgat gagtctgcgt cagcgacgta
    38701 aataatccct tcttatcgtg ccggaataca tcccatattt cggcacgctc accggatgcg
    38761 gcatcagtgc attatccgga taacaatatt ctcctatcca actttggcac atctattgct
    38821 ttgttataca aggcaaagct tgaaccagca tcagtacaac ccggttgcac cacagcaatc
    38881 caccggtagc accaaaagag tgcattttaa gaattttgct tcagaatggt gcggtgcacc
    38941 tcgcaaaaac aaacaataaa acatcaatat ctttacattt agccaaacta aatgttacct
    39001 aacaggcaca acactgcaca ataaagttgc agacgataac aacacaaaca ctcacaacgg
    39061 gtatccatgc gttcttaacg cagaagataa aggtgttgga tatgcaatta cgtaaacctg
    39121 ccacagcaat cctcgccctg gcgctttccg caggactggc acaggcagat gacgccgccc
    39181 cggcagcagg cagtacgctg gacaaaatcg ccaaaaacgg tgtgattgtc gtcggtcacc
    39241 gtgaatcttc agtacctttc tcttattacg acaatcagca aaaagtggtg ggttactcgc
    39301 aggattactc caacgccatt gttgaagcag tgaaaaagaa actcaacaaa ccggacttgc
    39361 aggtaaaact gattccgatt acctcacaaa atcgtattcc actgctgcaa aacggcactt
    39421 tcgattttga atgtggttct accaccaaca acgtcgaacg ccaaaaacag gcggctttct
    39481 ctgacactat tttcgtggtc ggtacgcgcc tgttgaccaa aaagggcggc gatatcaaag
    39541 attttgccga cctgaaaggc aaagccgtag tcgtcacttc cggcactacc tctgaagttt
    39601 tgctcaacaa actgaatgaa gagcaaaaaa tgaatatgcg catcatcagc gccaaagatc
    39661 acggtgactc tttccgcacc ctggaaagcg gtcgtgccgt tgcctttatg atggatgacg
    39721 ctctgctggc cggtgaacgt gcgaaagcga agaaaccaga caactgggat atcgtcggca
    39781 agccgcagtc tcaggaagcc tacggttgca tgttgcgtaa agacgatccg cagttcaaaa
    39841 agctgatgga tgacactatc gctcaggtgc agacctccgg tgaagcggaa aaatggtttg
    39901 ataaatggtt caaaaatcca attccgccga aaaacctgaa catgaatttc gaactgtcag
    39961 acgaaatgaa agcactgttc aaagaaccga atgacaaggc actgaactaa ttacaagaac
    40021 caggggcgga aattccagcc ctctcgattg ttacgtagca cggacagact atacgcctga
    40081 tggtcgttcc ccatcgggcc tgaaaaccgc aatacgctgg gtaacaatct tcgagggtag
    40141 cagttaacgc tgctaccctc ttttttctgg agtagattta tgtctataga ctggaactgg
    40201 ggtatttttt tacaacaagc cccgttcggc aataccacct atcttggttg ggtctggagt
    40261 ggttttcagg tgacgatcgc tttgtcgatc tgcgcctgga ttatcgcttt cctcgtcggt
    40321 tcatttttcg gcattttacg taccgttcca aaccgttttc tctccggtct gggtacgttg
    40381 tatgtcgaac tgttccgcaa cgtgccgttg attgtgcaat tctttacctg gtatctggtg
    40441 atcccggagc tgctgccgga gaaaatcggc atgtggttta aggccgagct ggatcccaat
    40501 attcagtttt tcctttcatc catgctctgc ctggggctgt ttaccgccgc ccgtgtttgc
    40561 gaacaggtac gcgccgctat tcagtcgcta ccgcgtgggc agaagaatgc cgcgctggcg
    40621 atgggcctga cgctaccgca gacttatcga tacgtactgc tgcctaatgc ttatcgcgtt
    40681 atcgtcccgc cgatgacttc cgagatgatg aacctggtga aaaactccgc catcgcctcg
    40741 accatcggtc tggtggatat ggcggcgcag gcgggtaaat tgctcgacta ctcggctcac
    40801 gcctgggagt cgtttaccgc catcacgctt gcgtatgtgt tgattaacgc ttttatcatg
    40861 ctggtgatga cgctggttga acgtaaagtt cgcctgcccg gcaacatggg gggcaaataa
    40921 tgtacgagtt tgactggagt tccattgttc cttccctgcc atatctgctc gacgggctgg
    40981 tgatcaccct gaaaatcacc gtcacggcag tcgtgattgg tattttgtgg ggtaccatgc
    41041 tggcggtgat gcgcttgtcc agcttcgcgc ctgtttcgtg gtttgccaaa gcctacgtta
    41101 acgtattccg ctcgattcct ttagtcatgg ttttgctgtg gttttacttg attgtgccgg
    41161 gatttctgca aaatgtgctg ggattatcgc caaaaaatga tattcgttta atttcggcga
    41221 tggtggcgtt ttcgatgttt gaagcggcct actattcaga gattatccgt gccggtattc
    41281 aaagtatttc ccgtggtcag tcgagcgccg cgctggcgtt ggggatgact cactggcagt
    41341 cgatgaaact gattattctg ccgcaggctt tccgcgcgat ggtgccgctg ctgctcactc
    41401 agggcatcgt actgttccag gatacctcgc tggtgtatgt gttatctctg gccgatttct
    41461 tccgtaccgc ctcaaccatt ggtgagcgtg atggtacgca ggtcgagatg atcctgtttg
    41521 ccggatttgt ttatttcgtt attagcctta gcgcgtcgtt gttggtcagc tacttgaaaa
    41581 gaaggacagc ataatgatta ccctgaaaaa tgtttcaaaa tggtatggtc actttcaggt
    41641 gctgaccgac tgctcaaccg aagtgaaaaa aggcgaagtg gtggtggttt gcggcccgtc
    41701 tggttccggc aaatcaacgc tgattaaaac cgtcaacggc ctcgaaccag tgcagcaagg
    41761 tgaaatcacc gtcgatggta tcgtggttaa cgacaagaaa accgatctgg caaagctgcg
    41821 ctcccgcgtc gggatggtat tccagcattt cgagctgttc cctcatctgt cgattatcga
    41881 aaacctgacc ctggcgcagg tgaaagtgct taaacgcgat aaagcgcccg ctcgtgaaaa
    41941 agcattgaaa ttgctggagc gcgtgggact ttccgcacat gccaataaat ttccggcgca
    42001 gctttccggc ggtcagcagc agcgcgtggc aatcgctcgc gcattgtgta tggctcctat
    42061 tgcgatgctg ttcgacgaac cgacatcggc gctggatccg gagatgatca acgaagtgct
    42121 ggacgtgatg gtggaactgg cgaacgaagg aatgaccatg atggtggtga cccacgaaat
    42181 gggctttgcc cgcaaagtgg cgaatcgggt gatctttatg gacgagggta aaattgtcga
    42241 agactcgccg aaagacgctt tcttcgatga tccgaaatcg gaccgcgcaa aagacttcct
    42301 cgcgaaaatc ctgcattaat cactctggcg cacataccca attgcgcgcc atctcacgct
    42361 ttatcgtttc gcccctcgta tctgttacat cctggcgtta cccttgtcgc aaagaagcac
    42421 acaacaagga gcaacaatgg cactgccaat tctgttagat tgcgacccag gtcatgatga
    42481 cgctatcgca atagttctcg cactcgcctc accagagctt gatgtcaaag caattacgtc
    42541 ttccgccgga aaccagacac cagaaaaaac cttacgcaat gttctgcgta tgctgacctt
    42601 gcttaatcgc accgatattc cggtggcagg cggcgcggta aaaccgttaa tgcgtgagtt
    42661 gattatcgcg gacaatgtgc acggcgaaag cggtctcgac ggcccggcat taccggaacc
    42721 gacgttcgca ccgcaaaact gtacggcggt ggagctgatg gcgaaaacgc tgcgcgaaag
    42781 tgcggaacct gtcacgattg tttctaccgg accgcaaact aacgttgcct tgctgctcaa
    42841 tagccatccg gaactgcata gcaaaattgc ccgtatcgtg attatgggcg gcgcaatggg
    42901 gcttggtaac tggacgcctg cggctgaatt taatatttac gttgacccgg aagcggcaga
    42961 aattgtcttc cagtcaggga tcccggtggt gatggccggt ctggatgtta ctcataaagc
    43021 gcaaatccac gttgaagaca ccgagcgttt ccgcgcgatt ggcaaccctg tttcaaccat
    43081 tgttgccgaa ctgctggatt tcttcctcga atatcataaa gacgaaaaat ggggctttgt
    43141 cggcgcaccg ctgcatgacc catgcaccat cgcctggctg ttgaaacccg agttgtttac
    43201 cactgttgag cgctgggttg gcgtggaaac acaggggaaa tatacccagg gtatgacggt
    43261 tgttgattat tattatctga ctggcaataa accgaatgcc actgtaattg tcgatgttga
    43321 tcgtcagggc tttgttgatt tactggctga tcgtctgaaa ttttacgctt aacactcgtc
    43381 attgattaag atcaccacgc gttacctggg tggtcttaat ctatttatct ctttttcttt
    43441 caagatataa atgcaatgga taatgcagaa ctcgccatag gtatcgatct cggcactacc
    43501 aatagtttaa ttgccgtctg gaaagacggt gccgcgcaat taattccaaa taagttcggt
    43561 gaatatttaa caccatccat aattagcatg gatgaaaata atcatatttt agtcggaaaa
    43621 ccggctgtat cacggcgtac ttcgcatccg gataaaacgg cagcgttatt taaacgtgca
    43681 atgggcagta ataccaactg gcggttaggc agcgacacat ttaacgcgcc agaactgtcc
    43741 tctttggtat tacgctcatt aaaagaagat gccgaagaat ttctgcaacg tccgattaaa
    43801 gatgtggtga tctccgttct ggcttatttc agcgatgaac aacgcaagca tacccgttta
    43861 gcagcggact tagccgggtt aaatgcggta cgcttaatta atgaacccac agcagctgcg
    43921 atggcgtatg gcctgcatac ccaacaaaat acccgttcgc tggtgtttga tctcggtggc
    43981 ggcacgtttg acgttacggt gcttgagtac gccacgccgg tgattgaagt tcacgcctcc
    44041 gctggcgaca acttcctcgg tggcgaagat tttacccata tgttggtcga tgacgtttta
    44101 aagcgcgcgg atgtcacaag gaccacgctt aacgagagtg aactggcagc cttgtacgcc
    44161 tgtgtggaag cggcaaaatg tagcaatcaa tcgccaatgc acattcgctg gtagtaccag
    44221 gatgaaacgc gggaatgcga attttacgag aacgaactgg aagatttgtg gttgccgctg
    44281 ctcaatcgct tgcgagtgcc gattgaacag gcgttgcgcg atgcgcgtct gaagccgagt
    44341 caaatcgaca gtctggtgct ggttggcggc gcgtcacaaa tgccgctggt gcagcgaatc
    44401 gccgtgcgtc tgtttggcaa attaccgtat caaagttacg atccgagcac cattgtcgcg
    44461 ctgggcgcag caatccaggc cgcctgccgc ttacgcagtg aagatattga agaagtgatc
    44521 ctcactgata tttgccctta ctcgttgggc gttgaggtta accgccaggg cgtttccggc
    44581 attttctcgc cgattattga acgaaacacc actgtgcccg tgtcgcgtgt agaaacttat
    44641 tcaaccatgc acccggaaca ggattcaatt acggttaacg tctatcaggg agaaaaccac
    44701 aaagttaaaa acaacattct ggtggaatcc ttcgatgtgc cgttgaagaa aaccggggct
    44761 tatcagtcga ttgatattcg ctttagttat gatatcaacg ggttgcttga agttgacgtg
    44821 cttctggaag acggcagcgt taagtccaga gtgattaacc acagcccggt aacattgagc
    44881 gcgcagcaga ttgaagagag tcggacgcgg ttatccgcat tgaaaattta tccgcgcgat
    44941 atgctcatca atcgcacctt taaagccaaa ctggaagagt tgtgggcgcg ggcgctgggt
    45001 gacgagcgag aagagattgg ccgggtgatc accgattttg atgcggcgct gcagtcaaac
    45061 gatatggccc gcgtcgatga ggtcaggcgg cgggcgagcg tttatttagc cattgagacc
    45121 tcataatcac ggctgccgga tgcggcgtaa atgctgcatc cggcatcggc gcaaaaaaca
    45181 catcgtcaac taaacaacat cgcgattttg ccgagagcat aacctataac cagcaccgtt
    45241 aaactgattt tgataatgct ggtaatgctc tgtggttttt caccctcatc atttttttct
    45301 tcatggtttt caggtgttgg ctcatcgaca tcttcatcct ccggctcgag ttttactttc
    45361 gtcaacagca tccgggtgat aatggcctct aatggatgag catctccgcc ttccagcctt
    45421 gtattaagac gggccagcat ctcttgtttc ggaaacgctt caagtactac ctgttccgct
    45481 acccgataca aatccagtaa cggtgaatct tcttctgtct cgctaccgac tctcgatttc
    45541 caccacgccc atgctttgcg tttgaagaat aacgactcaa taaacgattg acctaacggc
    45601 gataattttc cgctatgtaa aatttccgcc cataacagcc gcgtccgggc gctctcacca
    45661 ggaacatatt tcagcggttg accttgcgca tctaccatgc tccgcgcttc gataaccatc
    45721 actaataacg cgaaataatc cgggcaatga cgcttgctcc actgcaacaa caaattatca
    45781 gcttgtgtag atgggtaact ttcccagtac gcgccgagat cagcaagcag gctttccccc
    45841 tcgccgcagt acaaacgttg tgcacactgg taataccggg catcttcgct ttctggttct
    45901 tccgcttgcc actgtttggc tacaggaatg agttccacca tacctgtttg caccgagctg
    45961 taccagcgca acagtttgcg atgtaacttt gcatcatcag gaatgaacca cggtccttcc
    46021 atcgccaaaa acgcagtgac atagtcagga tggtaacggc agcaacgttc cagcgcgaag
    46081 taatattcaa cagtctggtt ctgtaacgcg acgggatatt ccagaaggct aaggaaatcg
    46141 aaacaatcac cagcctgaat ggcctcaaga aaagcctccc tttcttcctc atccattcct
    46201 tcgctgtcat taaacgaatg ccagttcaaa cgctctgcca acaaagaagc acaatttaat
    46261 gacagatatc gcgcttctat agcgattgca cacagcggcc agcgtaattg atcgacatcc
    46321 tccatgttcc aggtatttaa ttgctgaata aatttctgcc aggcggaggg ctgaaaacga
    46381 tcactttctg aatccagtaa tgtcctgaat gcacgtagta tttcatgttc agcggtggca
    46441 tctttttctt catcatctgc ttcctcgaca ggatttaccg ccagtcgcag cgcctcttca
    46501 taggcctggc gtaatgcttt aaacccctgc ggatcggttt cgggatgaca caacggtaag
    46561 cgagcaagat aagcctggcg gataatgtct atttgcgtcg tgctttcaat ttcgaggatt
    46621 tgccaacatg ttttcatagg tcgctcacat ccttaagcgt taccgggcat tcaggctgga
    46681 cagttaatgg ctgccaggga agcgtggcat agaaacggtc gcttcccgct gccagctctt
    46741 cgaacatccg actcccggcg ctatcgtatt tgtacagggt tagcgcctcg cgtagcgaag
    46801 cgttgtcttc gcactgagaa gattgccagt agagacgccc tagtgaatag gcagaaaaat
    46861 agtgcatcca gctgtgatag taatggtgag ctcgtgcata gacgcgcgat tgcagccata
    46921 aactctcttc ttccgtcagc cagttgttga gcacaccggc ccgtgacaga aatcccattc
    46981 gaacataatc ccaggctttg attccccctt ctccacagca catagcggtt tgggcaacga
    47041 attgggtata aatcaaccct ctctccgagc caccagcaat taatgctttc cactcttcag
    47101 gggaagagcg ttgccatgtc agatataagc cagctaaatc attggcatga ccatcatcag
    47161 tcatctgata aatagtctga attaaatcat cacgattatt tattccccag gatgatgaga
    47221 gatcgaattc ttttatcccg gtatacattt ttggcgagtc atgcgcgggt ttgcaactct
    47281 gattaagtat ttccatgggc gcagaaagcg caaaaagaag atattgttct tccttattca
    47341 ttttttaata atcccttaag tgcattaact gactactttt ttattattga tgagtataaa
    47401 cttgacgctt tcaaaataaa aagaaaatcg aagcattcac acatgaataa aaaattaatg
    47461 tatatattcg caatttttat agttgcagca attacctgta ttagccaacc caagaaaacg
    47521 atgttgcgtg ataaagccat ggtgaattat gcctttgatt atttaagatc accaggcagt
    47581 ctaccgttaa ccacggcagc cacggagctt tccgcgattc atggtcactc aacatcgcaa
    47641 tatcgccttg gcgaatttta tcttcatggt agcgacggta aaccactgga ttatacacag
    47701 gcgagatact ggtatgagca atcagcagaa caggaaaatc cgcgcgcaca aagcaaactg
    47761 gggtggatct acctgaaagg cctgggagtc aaacccgata cccgtaaagc aattctctgg
    47821 tataaggaag cagctgaaca agggtatgct catgctcaat atactttggg tttgatctac
    47881 agaaatggca ctggtattaa tgttaaccat tatgaatctc aaaaatggtt aaaactggcc
    47941 gccaaacaac attacaaaaa tgcggaaaga ttacttgccg gacttcccgc acattaacgt
    48001 cgactgtcac ccgaagagat ggagtatttt ccctatcaaa ccggcaaaga taaaaatgta
    48061 gaaaataatt ttgatgacgc ccagacagcc cagcccgcca tcatcaacgg gcattggctg
    48121 cggttgcggc tccggctcga cggtttcctg accggtaaaa ttcgccgcct gaattaataa
    48181 tcgacgaata attaatgctt ccagtggaca acgatcttct gcatcaaggc ggctcacaat
    48241 cggcattaat gattcttctg tcggttggct ggcctgcact aattgtttgg caactaaata
    48301 taatggccat aagggatgtt ctgtatcacg cgtgagtgag atccgcggct tacgtccttt
    48361 gtgcaaaacc atctcgacca gtgaacgccc cagcgcagag agtttttcat cgctgagtat
    48421 ttcgtataaa tgaaaacgcg tacgggcgct gtcgccgggg acataaagta gcggttttcc
    48481 ctgatcgtta accagatcac gcgcttcaat catcatcacc actaatgggt aataatagac
    48541 ccgatgctga cggcaccatt gcaacattaa agcatccgcc tgggtggagg gatactcgcg
    48601 ccagtagtaa caaagttctg ccagcaggct ttcgccttct ccacaataga cgcgttgtgc
    48661 gtattcataa taacgcgcat cttcattatc aggttcagct tcccgccacg accgggcgta
    48721 atccaggagt tcggggattc ccaggcggct tgcgctatac cagcggagta aatttctgtg
    48781 cagttttgca tcatcgggga taatcactgc gcgatgttgc gctaaataca atgtcagcca
    48841 ttctggatgc gatgaccaaa tccgagcgaa catttggtaa aaatcgatgg tgtcattttg
    48901 cacggcaact ggcagatgca gaatggtctg gaagttaaaa acattcccct tcgcaatggc
    48961 atataaaaag gattccagtt cttcttcgtc tatttccccg acgttttcct cctgcaacca
    49021 ccgcaatctt tctgctaata acaccacgca ctcgaaggat aaatgggcag tgttcatggc
    49081 gattgtgcac agcgaccagc gtaattcatc aatctcttcc atcgagcaat aatttaattg
    49141 ctgaatgaat cgctgccagg cggagggcag aaaacgttcg ctatcagagg caagtaacgc
    49201 gcgaaaggcg agcagaattt catgttctgc tacctcatat tcttctggtt gccaaacaga
    49261 tttagcaggc gactgcgcaa tccgtagcgc ctcctcatac gcctgacgaa gttgtttaaa
    49321 accctgcgga tcggtttctg gatgaaagga cggtaacagc gccagataag cgcggcggat
    49381 aatatcgacg tcagtcgttt cctctatatc gaggatcttc cagcaatttt tcataagtcg
    49441 ctcatatcct taagcgtttc cgggcatgtg gggtagtcag ccagtggttg ccaggacaag
    49501 gtagggtaat acgcatcatc actggcaaat aattgataaa acatatcatt gccggaatcg
    49561 tcgtattctt tacgcgctaa gaattcgcgt aataaaggca gatgatcatc ttcgggagac
    49621 tgccaatatt gccgaccaaa ggtataaccg gcaaagtatt gccgccagtt actataataa
    49681 cgtagagccc gtagatgaat acgcgactgg atccatagac tttcttcttc actcaaccag
    49741 ttattaagta cgcccattcg acttaaaaaa cccatccgga cataatccca tgctttaatt
    49801 cctccctcac cacagcattc ggctgtgctg gcgacaaatt gcgcataagc ctgaccttgc
    49861 tcatttaatt cagcaagata gtcacgccat tcacatggag aataacgaaa ccagcggtga
    49921 taaaaccctg ccaaccgagc ggcatgaccg ttctcgatca ttcggtgaat tatgcgcagc
    49981 aggtcatcac ggtcatcaat tccccatgag gaagaaaggt caaaaaagca tgtacctaaa
    50041 tacattttgg gggatgaatg cgaaggtaaa cagcattcat tgcgtatttc catcggagtg
    50101 gaaagtgcaa acaatatttt tgcgacccca tgtccatata atttatcctt aaacattcat
    50161 ttaatgaatg taaactacat ttcgaggctc agttatttat acttttctga cgcaacataa
    50221 tacgcataaa ataaggctta cgcaaaaatg gaacacataa aaatataata tattttatct
    50281 acaaccataa ggaaatgata atgattttta cgtcaagttg ctgcgataat ttatcaatag
    50341 atgagattat cgaacgtgct gaaaaaggcg attgcgaggc tcaatatatt gttgggtttt
    50401 attataatcg cgatagcgca attgactctc cggacgacga aaaggccttt tactggctga
    50461 agctggccgc tgagcaaggt cattgtgaag cacagtattc cttagggcaa aagtataccg
    50521 aggataaaag ccgtcataaa gataatgagc aagccatctt ctggctgaaa aaagctgccc
    50581 tacaaggcca tactttcgct tccaacgccc ttggctggat actggatcgt ggagaagacc
    50641 ccaactataa agaagcggtt acctggtatc agatagccgc ggagagcgga atgtcttatg
    50701 cgcaaaataa tcttggatgg atgtacagaa atggcaacgg agtcgcacaa gactatacgc
    50761 tggcattttt ctggtacaaa caagctgcat tacaaggcca tagttacgca caagacaatc
    50821 tggccgatct ttataaagac ggagaaggcg ttgctcaaaa caagacactc gccgcattct
    50881 ggtatttgaa aagcgcacag cagggtaatc ggcacgccca gtttcaaatt gcatgggatt
    50941 ataacgctgg cgaaggggtg gaccaggact ataagcaagc gatgtactgg tatctgaagg
    51001 ctgccgctca ggaaagcgtc ggcgcttacg tcaacatcgg ttatatgtat aaacacggac
    51061 aaggcgttga gaaagattat caggctgcct ttgaatggtt tacgaaagcc gctgaatgca
    51121 atgacgccac tgcctggtat aacctggcca ttatgtatca ctacggagaa ggaagacctg
    51181 tcgatctccg acaggctctc gacctgtatc gtaaagttca gtcatccgga accagagatg
    51241 tcagtcagga aattcgtgag actgaagctt tactgtagaa ataaaaacaa gggccgggga
    51301 atttcattct gcccatattc tctttattgc tgtgcgaaat cagactatta cggctcaaac
    51361 gggcgtctct ggaactggtc ataaccacat ttcgggcata gcgtcagcac ttccggtgtg
    51421 tagatcggga gatggaagtg acatttctcg cagaccagat ttcccagccc gaccacttct
    51481 ccgctgtgat aaaccccatg atgattgagg tcctggaaaa tttcgcgcca ttcaagctgc
    51541 gttttatcgg tgatgtctgc cagctcctgc cacaagcttt ctttaatcac ccgcataaag
    51601 acgctgtcag attcttcttt caggctctct tcatagctca tggcgaactc ttccaggtca
    51661 cgtctgacag ctcgcgtcag ctcatcgatc tcggttcgcg ttaactcccc tgtttttatt
    51721 acgcgctcgc gcgcctgttc caccagtgcg tcgatatcac gttcgccatt gcgcaggcgt
    51781 tcgttcagtg acgcgaccag ttcacggtaa tattgagcaa ccttgttcat cgtttcatct
    51841 cccgggtcgt taactgacta taataataga cgttctttgt tccttgcttt gctaatacgc
    51901 ccacaaactc tgtaaattaa tgtacgaata atccgggcat catttcaacg tcgagtgcgc
    51961 gaaaggctgt tttgacgcgg acgtttgggc tatgctatgc ggatctgaaa aaccacctca
    52021 acgctacatt tgtagccgta ttgaaaacag gaccactggc tgccatgcaa gagcaatacc
    52081 gcccggaaga gatagaatcc aaagtacagc ttcattggga tgagaagcgc acatttgaag
    52141 taaccgaaga cgagagcaaa gagaagtatt actgcctgtc tatgcttccc tatccttctg
    52201 gtcgactaca catgggccac gtacgtaact acaccatcgg tgacgtgatc gcccgttacc
    52261 agcgcatgct gggcaaaaac gttctgcagc cgatcggctg ggacgcgttt ggtctgcctg
    52321 cggaaggcgc ggcggtgaaa aacaacaccg ctccggcacc gtggacgtac gacaacatcg
    52381 cgtatatgaa aaaccagctc aaaatgctgg gctttggtta tgactggagc cgcgagctgg
    52441 caacctgtac gccggaatac taccgttggg aacagaaatt cttcaccgag ctgtataaaa
    52501 aaggcctggt atataaaaag acttctgcgg tcaactggtg cccgaacgac cagaccgtac
    52561 tggcgaacga acaagttatc gacggctgct gctggcgctg cgataccaaa gttgaacgta
    52621 aagagatccc gcagtggttt atcaaaatca ctgcttacgc tgacgagctg ctcaacgatc
    52681 tggataaact ggatcactgg ccagacaccg ttaaaaccat gcagcgtaac tggatcggtc
    52741 gttccgaagg cgtggagatc accttcaacg ttaacgacta tgacaacacg ctgaccgttt
    52801 acactacccg cccggacacc tttatgggtt gtacctacct ggcggtagct gcgggtcatc
    52861 cgctggcgca gaaagcggcg gaaaataatc ctgaactggc ggcctttatt gacgaatgcc
    52921 gtaacaccaa agttgccgaa gctgaaatgg cgacgatgga gaaaaaaggc gtcgatactg
    52981 gctttaaagc tgttcaccca ttaacgggcg aagaaattcc cgtttgggca gcaaacttcg
    53041 tattgatgga gtacggcacg ggcgcagtta tggcggttcc gggtcacgac cagcgcgact
    53101 acgagtttgc ctctaaatac ggcctgaaca tcaaaccggt tatcctggcg gctgacggct
    53161 ctgagccaga tctttctcag caagccctga ctgaaaaagg cgtgctgttc aactctggcg
    53221 agttcaacgg tcttgaccat gaagcggcct tcaacgccat cgccgataaa ctgactgcga
    53281 tgggcgttgg cgagcgtaaa gtgaactacc gcctgcgcga ctggggtgtt tctcgccagc
    53341 gttactgggg cgcgccgatt ccgatggtga cgctggaaga cggtaccgta atgccaaccc
    53401 cggacgacca gctgccggtg atcctgccgg aagatgtggt gatggacggc attaccagcc
    53461 cgattaaagc agatccggag tgggcaaaaa ctaccgttaa cggtatgcca gcgctgcgtg
    53521 aaaccgacac tttcgacacc tttatggagt cctcctggta ctatgcgcgc tacacttgcc
    53581 cagagtacaa agaaggtatg ctggattccg aagcggctaa ctactggctg ccggtggata
    53641 tctacattgg tggtatcgaa cacgccatta tgcacctgct ctacttccgc ttcttccaca
    53701 aactgatgcg tgatgcaggc atggtgaact ctgacgaacc agcgaaacag ttgctgtgtc
    53761 agggtatggt gctggcagat gccttctact atgttggcga aaacggcgaa cgtaactggg
    53821 tttccccggt tgatgctatc gttgaacgtg acgagaaagg ccgtatcgtg aaagcgaaag
    53881 atgcggcagg ccatgaactg gtttataccg gcatgagcaa aatgtccaag tcgaagaaca
    53941 acggtatcga cccgcaggtg atggttgaac gttacggcgc ggacaccgtt cgtctgttta
    54001 tgatgtttgc ttctccggct gatatgactc tcgaatggca ggaatccggt gtggaagggg
    54061 ctaaccgctt cctgaaacgt gtctggaaac tggtgtacga gcacacagca aaaggtgatg
    54121 ttgcggcact gaacgttgat gcgctgactg aagatcagaa agcgctgcgt cgcgatgtgc
    54181 ataaaactat cgctaaagtg accgatgata tcggccgtcg tcagaccttc aacaccgcaa
    54241 ttgcggcgat tatggagctg atgaacaaac tggcgaaagc accgaccgat ggcgagcagg
    54301 atcgcgctct gatgcaggaa gcactgcagg ccgttgtccg tatgctcaac ccgttcaccc
    54361 cgcacatctg cttcacgctg tggcaggaac tcaaaggcga aggcgatatc gacaacgcgc
    54421 cgtggccggt tgctgacgaa aaagcgatgg tggaagactc cacgctggtc gtggtgcagg
    54481 ttaacggtaa agtccgtgcc aaaatcaccg tcccggtgga cgcaacggaa gaacaggttc
    54541 gcgaacgtgc tggccaggaa catctggtag caaaatatct tgatggcgtt actgtacgta
    54601 aagtgattta cgtaccaggt aaactcctca atctggtcgt tggctaagcg cgggaggaag
    54661 cgtgcgatat ctggcaacac tgttgttatc tctggcggtg ttaatcaccg ccgggtgtgg
    54721 ctggcatctg cgtgacacca cgcaggttcc ttccactatg aaggtcatga tcctggactc
    54781 aggcgatccg aacgggccat taagccgtgc ggtgcgtaac cagttacgtc tgaatggtgt
    54841 cgagttgctt gataaagaaa ccacgcgtaa ggacgttcca tccctgcgtt tgggtaaagt
    54901 gagcatcgcg aaagataccg catcggtatt ccgtaacggt caaacagcag agtatcagat
    54961 gatcatgacg gtgaatgcga ccgtgttgat ccccggccgt gatatctacc cgattagcgc
    55021 caaagtcttc cgttcattct tcgataaccc gcaaatggcg ttagcgaaag ataacgaaca
    55081 agacatgatc gtaaaagaga tgtacgaccg tgctgccgaa cagctgattc gtaagctgcc
    55141 aagcatccgt gctgcggata ttcgttccga cgaagaacag acgtcgacca caacggatac
    55201 tccggcaacg cctgcacgcg tctccaccat gctgggtaac tgatgattcg gttgtacccg
    55261 gaacaactcc gcgcgcagct caatgaaggg ctgcgcgcgg cgtatctttt acttggtaac
    55321 gatcctctgt tattgcagga aagccaggac gctgttcgcc aggtagctgc ggcacaagga
    55381 ttcgaagaac accacacttt ttccattgat cccaacactg actggaatgc gatcttttcg
    55441 ttatgccagg ctatgagtct gtttgccagt cgacaaacgc tattgctgtt gttaccagaa
    55501 aacggaccga atgcggcgat caatgaacaa cttctcacac tcaccggact tctgcatgac
    55561 gacctgctat tgatcgtccg cggtaataaa ttaagcaaag cgcaagaaaa tgccgcctgg
    55621 tttactgctc ttgcgaatcg cagcgtgcag gtgacctgtc agacaccgga gcaggctcag
    55681 cttccccgct gggttgctgt gcgcgcaaaa cagctcaact tagaactgga tgacgcggcg
    55741 aatcaggtgc tctgctactg ttatgaaggt aacctgctgg cgctggctca ggcactggag
    55801 cgtttatcgc tgctctggcc agacggcaaa ctgacattac cgcgcgttga acaggcggtg
    55861 aatgatgccg cgcatttcac cccttttcat tgggttgatg ctttgttgat gggaaaaagt
    55921 aagcgcgcgt tgcatattct tcagcaactg cgtctggaag gcagcgagcc ggttattttg
    55981 ttgcgcacat tacaacgtga actgttgtta ctggttaacc tgaaacgcca gtctgcccat
    56041 acgccactgc gtgcgttgtt tgataagcat cgggtatggc agaaccgccg gggcatgatg
    56101 ggcgaggcgt taaatcgctt aagtcagccg cagttacgtc aggccgtgca actcctgaca
    56161 cgaacggaac tcaccctcaa acaagattac ggtcagtcag tgtgggcaga gctggaaggg
    56221 ttatctcttc tgttgtgcca taaacccctg gcggacgtat ttatcgacgg ttgatatgaa
    56281 atctttacag gctctgtttg gcggcacctt tgatccggtg cactatggtc atctaaaacc
    56341 cgtggaaacg ctggcgaatt tgattggtct gacgcgggtc acaatcatcc ctaataatgt
    56401 tcctccgcat cgtccccagc cggaagcgaa cagcgtgcag cgtaaacaca tgcttgaact
    56461 ggcgattgcc gacaagccat tatttactct tgatgaacgc gagctaaagc gcaatgcccc
    56521 ctcttacact gcgcaaacac tgaaagagtg gcggcaggaa caaggaccgg acgtgccgct
    56581 ggcgtttatt attggtcagg attcattgct gacctttccg acctggtacg aatacgaaac
    56641 gatactcgac aatgcacatt tgatcgtctg tcggcgtcca ggttacccac ttgaaatggc
    56701 gcaaccgcaa taccagcaat ggctggaaga tcatttgaca cataacccgg aagatcttca
    56761 ccttcagcct gccggtaaaa tttatctggc tgaaacgccg tggtttaaca tctcggcgac
    56821 catcatccgc gaacgtttgc aaaacggtga atcgtgtgag gatttattgc cggaaccggt
    56881 attgacttac attaaccaac aaggcttgta tcgctgatac ctgtcgttac ggagggcatg
    56941 atgcgactgt ggttaattcg tcatggcgaa acacaagcga atgtcgatgg tctgtacagc
    57001 ggtcatgcgc ccacacccct gaccgcacgc ggtattgagc aagcgcaaaa tctgcataca
    57061 ctgctagatg atgtttcctt tgacttggtt ttatgcagtg aactggaacg ggcacagcat
    57121 accgcgcgac tggttctcag tgaccgccag ctccccgttc atatcatccc tgaactcaac
    57181 gaaatgtttt ttggcgactg ggagatgcga catcatcgcg acctcatgca agaagatgcc
    57241 gaaaactata gcgcgtggtg caatgactgg cagcatgcta tccccacgaa cggtgaagga
    57301 tttcaggcat tttcgcaacg tgtggaacgc tttatcgcaa ggcttagtga atatcagcac
    57361 tatcagaata ttttaatcgt cagccatcag ggcgtactga gtctgttaat tgctcgttta
    57421 attggcatgc ctgccgagtc tatgtggcat tttcgcgttg accaggggtg ttggagcgcc
    57481 attgatatca accaaaaatt cgcgacgcta cgcgtcctca atagccgtgc catcggggtc
    57541 gagaacgcat gacttttctg tttttttacg ggtaagccgc aacgaccatt gacagccccg
    57601 ggcaggctga tattctccgc agccagactt tttccgccag acacgacttt gtagaaattg
    57661 ttttacaaaa atggcgatgc aatctgcggc gcggggtggg atgatagccc actttcgaaa
    57721 gccgattcgg cgacaattgt cccgaaatcg cctctggttc aggtatactg acagaccatt
    57781 tttatctatt tgattcaccc agggggaaaa cttgcagggt aaagcactcc aggattttgt
    57841 tatcgacaaa attgatgacc tcaaaggtca ggacatcatc gccttagacg ttcaaggcaa
    57901 atccagcatc accgactgca tgatcatctg tacgggtacg tccagccgtc atgttatgtc
    57961 cattgctgac cacgttgtgc aggagtctcg cgcagcgggc ctgttaccgc tcggcgtaga
    58021 aggtgaaaac agcgccgact ggattgtcgt ggatttgggc gatgtgattg tccatgtcat
    58081 gcaggaagag agccgtcgcc tgtatgaact ggaaaaactc tggagttaat gcgtgaagct
    58141 gcaacttgtc gccgtgggaa cgaaaatgcc ggactgggta caaaccggtt ttaccgagta
    58201 cctgcgtcgt tttccgaaag atatgccctt cgagctgatt gaaattccgg ccggcaaacg
    58261 cggcaagaat gcggacatca agcgcatact cgacaaagag ggtgagcaga tgttggcggc
    58321 cgcaggcaaa aaccgcattg tcaccctcga tattccaggc aagccctggg atacgccgca
    58381 gttagccgct gagctggaac gctggaagct ggatggtcgc gacgtaagtc tactgattgg
    58441 cgggcctgaa gggttgtcgc ctgcctgtaa agcggcggct gagcagagct ggtcgctgtc
    58501 ggcgcttacc ctcccccatc cgctggttcg cgtgctggtc gcagagagtc tgtaccgggc
    58561 gtggagcatc accaccaacc atccttatca ccgtgagtga taagggagct ttgagtagaa
    58621 aacgcagcgg atgaaactac agaactcttt tcgcgactat acggcagagt ccgcgctgtt
    58681 tgtgcgccgg gcgctggtcg cctttttggg gattttgctg ctgaccggcg tgcttatcgc
    58741 caacctgtat aatctgcaaa ttgttcgctt taccgactac cagacccgct ctaatgaaaa
    58801 ccgcattaag ctggtgccta tcgcgcccag ccgcggcatt atctacgacc gtaacggtat
    58861 ccctctggcc ctcaaccgca ctatctacca gatagaaatg atgccggaga aagtcgataa
    58921 cgtgcagcaa acgctggacg ctttgcgcag cgtggtagat ctgaccgatg acgatattgc
    58981 tgcattccga aaagagcgcg cacgttcaca ccgtttcacc tctattccgg tgaaaactaa
    59041 cctaaccgaa gtacaagtag ctcgctttgc cgtcaatcag taccgttttc cgggtgtcga
    59101 agttaaaggc tataaacgtc gttactatcc ttacggttcg gcgttgaccc acgtcatcgg
    59161 ctatgtgtcg aaaatcaacg ataaagacgt cgaacgcctg aataatgacg gcaaactggc
    59221 caactatgcg gcaacgcatg atattggtaa gctgggcatt gagcgttact atgaagacgt
    59281 gctgcacggt cagaccggtt atgaagaggt tgaagttaac aaccgtgggc gtgttatccg
    59341 ccagttaaaa gaagtaccac cgcaagccgg acacgatatt tacctgacgc tggatctcaa
    59401 actccagcaa tatattgaaa cgctgctggc gggtagccgc gcagctgtgg ttgttaccga
    59461 accgcgtaca ggtggggtgc tggcgctggt ttccacgcct agttatgacc caaacctgtt
    59521 tgttgacggt atctccagca aagattattc cgccttgctg aacgacccga atacaccgct
    59581 ggtgaaccgc gccacacagg gggtttatcc tcccgcgtct acagttaaac cctatgtggc
    59641 ggtttcggca ttaagcgccg gggtgatcac gcgcaatacg acgctgtttg acccaggctg
    59701 gtggcaacta ccagggtcgg aaaaacgtta tcgtgactgg aaaaaatggg gccacgggcg
    59761 tctgaatgtc acaagatcgc tggaagaatc tgcggatacc ttcttctatc aggtggccta
    59821 cgatatgggg atcgatcgcc tctccgaatg gatgggtaaa ttcggttatg gtcattacac
    59881 cggtatcgac ctggcggaag aacgttccgg caacatgcct acccgcgaat ggaaacagaa
    59941 acgctttaaa aaaccgtggt atcagggtga caccattccg gttggtatcg gccagggtta
    60001 ctggacagcg accccaatcc agatgagtaa ggcactgatg atcctgatta atgacggtat
    60061 cgtgaaggtt ccgcatttgc tgatgagcac cgccgaagac ggcaaacagg tgccatgggt
    60121 acaaccgcat gaaccgcccg tcggcgatat tcattccggt tactgggagc tggcgaaaga
    60181 cggtatgtac ggtgttgcta accgccctaa tggtacggcg aataaatact ttgccagcgc
    60241 accgtacaaa attgcggcga aatctggtac cgctcaggtc ttcggtctga aagcgaacga
    60301 aacctataat gcgcacaaaa ttgccgagcg tttacgtgac cacaaactga tgaccgcctt
    60361 tgcgccatac aacaatccgc aagtggctgt cgccatgatt ctggagaacg gcggcgcggg
    60421 tccggcggtt ggtacgctga tgcgccagat cctcgaccac attatgctgg gtgataacaa
    60481 caccgatctg cctgcggaaa atccagcggt tgccgcagcg gaggaccatt aatcatgacg
    60541 gataatccga ataaaaaaac attctgggat aaagtccatc tcgatcccac aatgctgctg
    60601 attttactgg cattgctggt ttacagcgcc ctggttatct ggagcgccag cggtcaggat
    60661 attggcatga tggagcgtaa aatcggccaa atcgcgatgg gtctggtcat catggtggtg
    60721 atggcgcaaa ttcctccacg cgtttatgaa ggctgggccc cctatctcta tatcatctgt
    60781 attattttgc tggtggcggt agatgctttc ggagccatct ctaaaggcgc tcaacgctgg
    60841 ctggacctcg gtattgttcg ttttcagccg tcggaaattg ccaaaatagc cgtaccactg
    60901 atggttgcgc gctttatcaa ccgcgacgtt tgcccgccat cgttgaagaa tactggcatc
    60961 gcgctggtgc tgatatttat gcccacgctg ctggtggctg cacagcctga cctgggaaca
    61021 tcaatcctcg ttgcgctttc cggtctgttt gtactgttcc tctctggcct tagctggcgt
    61081 ctgattggcg tcgcagtagt gctggtagcg gcgttcattc cgattctgtg gttcttcctg
    61141 atgcatgatt accagcgcca gcgcgtaatg atgctcctgg acccggaatc agacccactc
    61201 ggcgcgggct atcacattat tcagtctaaa attgctattg gctccggcgg attacgcggc
    61261 aaaggctggc tgcacggcac tcagtcacag cttgaatttc tccccgaacg ccatactgac
    61321 tttatcttcg cggtactggc ggaagagctg ggattagtgg gcattctgat tctgctcgct
    61381 ctctacattc tgctgatcat gcgcgggctg tggatagccg ccagagcgca aaccaccttt
    61441 ggtcgcgtca tggctggtgg cttaatgctg atattattcg tttatgtctt cgtaaatatt
    61501 ggtatggtaa gcggtattct gccggttgta ggggttccgc tcccactggt cagttatgga
    61561 ggatcggcgc taattgtgct gatggctggg ttcgggattg taatgtcaat ccacacccac
    61621 aggaaaatgt tgtcgaaaag cgtgtaagag gtgcgcaatg cgtaagcagt ggctcgggat
    61681 ctgcatcgcg gcaggaatgc tcgcggcatg tacaagcgat gatggtcagc aacagacggt
    61741 aagtgtaccg cagcctgcgg tatgtaacgg ccccatagtt gaaattagcg gggcggaccc
    61801 gcgtttcgaa ccactgaacg cgacggcaaa tcaggattac cagcgcgacg gtaaaagcta
    61861 caaaatcgtg caggatccgt ctcgatttag ccaggcggga ctggcggcaa tctatgatgc
    61921 cgaaccaggc agtaacctga cggcctctgg cgaagctttc gatccgacaa agctgacggc
    61981 ggcccatcca acgcttccga tccccagcta cgccagaatc actaacctgg ctaacgggcg
    62041 aatgatcgtg gtgcgcatta atgatcgcgg cccttacggc aacgaccgcg ttatttcgct
    62101 ttctcgcgcg gcagctgacc gtcttaacac gtcaaacaac accaaagttc gtatcgatcc
    62161 gattattgtc gcccaggatg gttcgctttc tggtcctggt atggcttgta ccacagtcgc
    62221 caaacagact tacgccctgc ctgcacctcc cgatttaagc ggtggcgcgg gaacaagttc
    62281 agtgtctggc ccgcagggtg acattcttcc ggtcagtaat tcgacgctaa aaagcgaaga
    62341 tccgaccggc gcgccggtaa ccagtagcgg tttcctcggc gctccaacga ccttagcgcc
    62401 tggtgtactg gaaggcagcg aaccgacgcc tgcgccacag cccgttgtta cagcttcgtc
    62461 gacaacgcct gcaacctcgc ctgcaatggt gacaccgcaa gccgcctcgc aaagcgccag
    62521 cggcaacttt atggtgcaag tcggggccgt aagcgatcag gctcgtgcgc aacagtacca
    62581 acagcaactg ggacagaagt tcggcgtccc cggtcgcgta actcaaaatg gcgcggtctg
    62641 gcggatccag cttggcccat tcgccagcaa agccgaagcc agtaccttgc agcaacgttt
    62701 gcaaaccgaa gcccaattac agtcatttat tactaccgcg cagtagcgta aagcaggcat
    62761 ctgatgtgtc aatttctgta aatgacgtta acaaagtcac gcggaaagtc agatgcctgc
    62821 cggtagtgca tttgctatag tagggcactt ttttaattcc atcacggatg tcgtagttca
    62881 gaccatgaat accatttttt ccgctcgtat catgaagcgc ctggcgctca ccacggctct
    62941 ttgcacagcc tttatctctg ctgcacatgc cgatgacctg aatatcaaaa ctatgatccc
    63001 gggtgtaccg cagatcgatg cggagtccta catcctgatt gactataact ccggcaaagt
    63061 gctcgccgaa cagaacgcag atgtccgccg cgatcctgcc agcctgacca aaatgatgac
    63121 cagttacgtt atcggccagg caatgaaagc cggtaaattt aaagaaactg atttagtcac
    63181 tatcggcaac gacgcatggg ccaccggtaa cccggtgttt aaaggttctt cgctgatgtt
    63241 cctcaaaccg ggcatgcagg ttccggtttc tcagctgatc cgcggtatta acctgcaatc
    63301 gggtaacgat gcttgtgtcg ccatggccga ttttgccgct ggtagccagg acgcttttgt
    63361 tggcttgatg aacagctacg ttaacgcact gggcctgaaa aatacccact tccagacggt
    63421 acatggtctg gatgctgatg gtcagtacag ctccgcgcga gatatggcgc tgatcggcca
    63481 ggcattgatc cgtgacgtac cgaatgaata ctcgatctat aaagaaaaag aatttacgtt
    63541 taacggtatt cgccagctga accgtaacgg cctgttatgg gataacagcc tgaatgtcga
    63601 cggcatcaaa accggacaca ctgacaaagc aggttacaat cttgttgctt ctgcgactga
    63661 aggccagatg cgcttgattt ctgcggtgat gggtggacgt acttttaaag gccgtgaagc
    63721 cgaaagtaaa aaactgctaa cctggggctt ccgtttcttc gaaaccgtta acccactgaa
    63781 agtaggtaaa gagttcgcct ctgaaccggt ttggtttagt gattctgatc gcgcttcgtt
    63841 aggggttgat aaagacgtgt acctgaccat tccgcgtggt cgcatgaaag atctgaaagc
    63901 cagctatgtg ctgaacagca gtgaattgca tgcgccgctg caaaagaatc aggtcgtcgg
    63961 aactatcaac ttccagcttg atggcaaaac gatcgagcaa cgcccgctgg ttgtgttgca
    64021 agaaatcccg gaaggtaact tcttcggcaa aatcattgat tacattaaat taatgttcca
    64081 tcactggttt ggttaaaaat tgaacacttg aaagtgtaat ttccgtcccc atatactaag
    64141 catcagtaaa aaaactcccg ccttctggcg ggagttgcta tttaattacg ttacgccgga
    64201 gctgacatga aaaccaaact taacgaactg cttgaattcc ctactccttt tacttacaaa
    64261 gttatggggc aggcgttacc tgagctggtt gatcaggtgg ttgaagtggt acagcgccat
    64321 gcgccaggtg actacacccc aacggtaaaa ccaagcagca aaggcaacta ccactcggta
    64381 tctatcacta tcaacgccac tcatatcgag caggttgaaa cactgtatga agaactgggc
    64441 aaaatcgata ttgtccgcat ggttctgtaa ctcgcttctc cgttacccgg ctcctggtcg
    64501 ggtaactccc ctctcctcgc tgtgatatac ttttccccca cttttactca ttctccacgg
    64561 agatgccgtt ttgtatcagg ataaaattct tgtccgccag ctcggtcttc agccttacga
    64621 gccaatctcc cgggctatgc atgaattcac cgatacccgc gataatagta cccttgatga
    64681 aatctggctg gtcgagcact atccggtatt cacccaaggt caggcaggaa aagcggagca
    64741 cattttaatg ccgggtgata ttccggtgat ccagagcgat cgcggtgggc aggtgactta
    64801 tcacgggccg ggacaacagg tgatgtatgt gttgcttaac ctgaaacgcc gtaaactcgg
    64861 tgtgcgtgaa ctggtgacct tgcttgagca aacagtggtg aataccctgg ctgaactggg
    64921 tatagaagcg catcctcggg ctgacgcgcc tggtgtctat gtcggggaaa agaaaatttg
    64981 ctcactgggt ttacgaattc gacgcggttg ttcattccac ggtctggcat taaacgtcaa
    65041 tatggatctt tcaccatttt tacgtattaa tccttgtggg tatgccggaa tggaaatggc
    65101 taaaatatca caatggaaac ccgaagcgac gactaataat attgctccac gtttactgga
    65161 aaatatttta gcgctactaa acaatccgga cttcgaatat attaccgctt aattccatac
    65221 atcaatggcc caatttacac tgggtcatta ctcataatac ctgctaatgc tttacatctc
    65281 cttcgcgttt gtatattttc tttatcactt gcagaaatgc ggtgccacgc cttgctccgt
    65341 aactcacccc attgattaac ctattgaaat ggttgattat ggtgagcaaa agcgttccgt
    65401 tccaattaga cataagatta ttgtaatttt ttaaatatta tttttccatg aataaaattg
    65461 gagtaagtgc gtggatagta ataatcaaat tgaaccctgt ttaagcagaa agtcgtcaga
    65521 aggtaaacca caaatattta caaccttacg aaatattgat cttaaccttc tgactatttt
    65581 tgaagctgta tatgtacata aagggatcgt taatgcagcg aaagtgctta atctgacccc
    65641 ctcggcaatc agtcagtcta ttcagaaact gcgcgttata ttccctgacc cattgtttat
    65701 tcgcaaaggc cagggtgtca ctcctaccgc atttgcgatg catctacatg agtatatcag
    65761 tcagggcctt gagtccattc ttggcgcgct ggatatcgaa ggaagctatg ataagcaacg
    65821 aacgataact attgcgacca ctccctcggt cggagccctg gtccttcctg tcatctatcg
    65881 ggcgattaaa actcactatc cgcagctttt actgcgcaac ccgcccatca gcgacgcgga
    65941 aaaccaactc agtcagtttc aaaccgatct catcatcgat aacatgtttt gcaccaatcg
    66001 tacggtgcaa catcatgttc tgttcaccga caatatggtg ttaatttgcc gcaagggaaa
    66061 tccactactc tctttagaag atgacagaga gactatcgac aacgctgcgc atgtacttct
    66121 gttaccggaa gggcaaaatt tcagcggtct gcggcagaga gttcaagaga tgtttccgga
    66181 ccggcaaatc aatttcacca gctacaacat tttgacaatc gctgcactgg ttgccaacag
    66241 tgacatgtta gcgattattc ccagccgttt ttataacctg tttagtcgtt gctggccgct
    66301 ggaaaaactg ccttttccgt ccttaaatga ggagcaaata gatttctcta tccactacaa
    66361 caaattcatc ctgcgtgatc ccatcctgca cggagtaata gatgttatcc gtaatgcatt
    66421 ttgaaaaagt attgttacag ccagatcaca gcgcagaacg ataaaatggc acaatccaac
    66481 aacaatttta cattttcgcg accgctttgg ctgctttcag gtccgtttca atgatatact
    66541 gccagtcgtt aattcaaaaa tagttgataa ttacaacaat ctattgaatt gaaacgcttt
    66601 ccttcgtaat tcgcaactgg aacacgcacg ctatgagtaa acccattgtg atggaacgcg
    66661 gtgttaaata ccgcgatgcc gataagatgg cccttatccc ggttaaaaac gtggcaacag
    66721 agcgcgaagc cctgctgcgc aagccggaat ggatgaaaat caagcttcca gcggactcta
    66781 cacgtatcca gggcatcaaa gccgcaatgc gcaaaaatgg cctgcattct gtctgcgagg
    66841 aagcctcctg ccctaacctg gcggaatgct tcaaccacgg cacagcaacg tttatgatcc
    66901 tcggcgctat ttgtacccgc cgttgtccgt tctgtgatgt tgcccacggt cgcccggtag
    66961 ctcctgatgc caatgaacca gtgaaactgg cgcagaccat tgccgatatg gcgctgcgtt
    67021 atgtggttat cacctccgtt gaccgtgatg acctgcgcga tggcggtgcc caacactttg
    67081 cggattgtat tactgccatt cgggaaaaaa gcccgcaaat caaaattgaa actctggtgc
    67141 cggatttccg cggtcgaatg gatcgtgctc tggatattct gactgcaacg cccccagatg
    67201 tgttcaacca taacctggaa aacgtaccgc gtatttaccg gcaggtacgg cctggcgcag
    67261 attacaactg gtcgctgaag ctgctggaac gctttaaaga agcgcatccg gaaatcccga
    67321 ccaagtctgg tctgatggtg ggtctgggtg aaaccaatga agaaattatt gaggtaatgc
    67381 gcgacctgcg ccgtcatggt gtgacgatgt taacgctggg gcaatatttg cagccaagcc
    67441 gccatcacct gccggttcaa cgttacgtta gcccggatga gttcgacgaa atgaaagccg
    67501 aagcgctggc gatgggcttt acccatgctg catgcggtcc gtttgtccgc tcttcttacc
    67561 acgccgattt gcaggcgaaa ggaatggaag ttaagtaatc ctgtaataat tacttacatc
    67621 tgtctgataa aaaacccgct ttttgaagcg ggttttttgt atcgaacaag atattgaggg
    67681 agcgtcctgc tcgccacgtc actctttatg agagagcttt tcagcctgaa gatcaacgtc
    67741 tgcgcctttt ttcgccgcag catcgtcatc attcatcgcc ttcttgaacc ctttaatggc
    67801 cgctccaagg tctccgccca gcgtacgtaa cttcttagtc ccaaacagca gaacgaccag
    67861 cgccgcaact accagcagtt tggtaatact aatctcaccc atagatacct tcttgacata
    67921 taaccggcaa cagaacgcgc cttaaaaacc agagaatatt aacggtcatt cgacgcgcgc
    67981 acacaatagc aatctgtaac aaggtgaatc aagcgccgag taaaaaaaca tcataataat
    68041 tgcggcggcg caaagcgacg gttgtttaag atgggcagtt gcgcgcgcac ctgacgcaca
    68101 cgttcgggcg tcacttccgc cataatgagt gcaggcattt ctgacgctgc cgcaatggtg
    68161 acgccaaagg gatctataat ccggctttgc ccgatatttt tgttcccgca ctcccccgcc
    68221 gccaccatat aacaggtggt atccagcgca cgagcggcaa gcaacgttga ccagtgatgc
    68281 tctttgagcg gcccacgaac ccaggcggca ggaagtacca ggatttcagc tccctgtaaa
    68341 gcctgtgcca gcgccagctc tggaaagcgt aagtcataac aggtcatcag accgaccttc
    68401 atcccttcca cctccagtaa cggagctatt tcattaccag catcaacatg gcgtgattcc
    68461 tgaatggcaa atgcatcata gagatgcagt ttggcataac gggcgacgat gtttcctgcc
    68521 tgaagtgcca ccagcatatt ccatgcgcgc cccggcgttg aaggaagatg aatcgtcaga
    68581 attgtcgtca tcatgttacg tttactttct cgccgtaaac gtccgaggaa ttcgccttcc
    68641 agcagctgtg ctgatttaac cgatagatct gcatcatgat catcgcgcgc cagcaatgct
    68701 tccggcagga caaacagcga tacgtcgttt tccgccgcct gcgccatcaa cgaggcacaa
    68761 atctcagcgt tcttttccca cacagatgta acagcaaact gtcctgcagc aaccagcatt
    68821 gccacctcct gtcagagtcg taaaattcgc caacgacgat tattcggcgt tacacttatc
    68881 actcatacaa atcaaatagc aggattttgc agtgttacaa cttcttttag cagtttttat
    68941 tggcggtggt acgggaagcg tagcgagatg gctgttaagt atgcgattta acccgctgca
    69001 tcaggcgatt ccgttgggga cgctggcagc aaacctgatt ggggcattca tcataggaat
    69061 gggattcgcc tggttcagca ggatgacgaa cattgatcca gtgtggaaag tattaatcac
    69121 caccggattt tgtggcggtc taacaacctt ctcaacattt tcggcagaag tggtgttttt
    69181 gttacaagag ggccgctttg gctgggcatt actgaacgtt ttcgtcaacc ttctggggtc
    69241 ttttgccatg accgcactgg cattctggct gttttcggcc tcaaccgtac actaaagacc
    69301 aaaaaaaacc cgctgattaa gcgggttttg aattcttgct gacgtatctt acagagcgat
    69361 tacgtttgca gcagaagggc ctttggcacc gttagtgatt tcgaactcta cgcgctgacc
    69421 ttcagcaaga gttttaaaac cattagtctg gattgcagag aagtgtacga acacgtcttt
    69481 gctgccgtct tccggagtaa tgaaaccgaa tcctttggac tcattaaacc acttaacgtt
    69541 acctttaatc ttagacatca aaattacctt tacatgaaaa atagacacaa atgctgtgtc
    69601 ggttatcagt acaccaattg tggtacgctt tgtccagtgc aactttacta aaaagtgata
    69661 aaagtcgcgt tatttttgat gttgcttatt ttagttattg attttaaaga agttactaaa
    69721 acttcatttg tctcaaaact gaaagcgcat ccaggcaaag tacacattgc cattgttgta
    69781 ggtacccgga atgtaggtca tctgaaaagt cactgggcca taacccacgg aggccaatgg
    69841 cagtagaacc gggagaggga tgtaattcca gttatcgcgt gccgttacgc cagcggtgaa
    69901 tcccagacct aaatgaaaat tttcatccgc cagcggtcgc caggtacttt cccatccgta
    69961 tccggcaatc ggttcccatt tgttccacga gtccttaaat gccatggcat acaggccatg
    70021 ccagtttcct ttttcatccc aacgcgacag gccaaaaccg ccaccccacg gtcgctcgtt
    70081 atagcgatcg gttttttctt tgtcgtaagc gaaacgtgca tgccaggtga tggcaggaat
    70141 atataaatca taatgttcag gctgttgcca ggtttgtgca atattttctc taaacgttgt
    70201 catcctctca tctgcgttag caaaaacttt accaacgctg attaactgaa taaaaacaaa
    70261 ggaaaagata gcgacatatt tactcacgtt catttgtgac cataaaacat ttatcaaaaa
    70321 tctactacta gcatagcaaa gctacaatta acataacctt aatagaccca acataaagaa
    70381 taatctgaat agctgctcta tttatttccg cttgttcaag ttaaatattt ccgcgcatcg
    70441 attaaagatg atgcgtcgtt ttctgcgatg ggaatagtca aaaaagaaaa accaagtctt
    70501 ttttgatgac aaaatgcaat caaggaaaaa ttattttatt tttttaacga gatacaacaa
    70561 tcatcttaac gaagtatata atatatatcg tcaacccatg ctcatattag attatttttt
    70621 gagcgcacag ccgcagctaa caccattgca attaacaaat ttgcatcaat ccaccatcaa
    70681 tttacacaca ttattatgtg ataattgcca accgctaaat atgcgttttg ttatctatgt
    70741 ataaaaacag caacttcaat gtcttaatgg cagtttttct tgattttaat cagcattcat
    70801 cgccaattta ttgggcatat tttttcctta agctttagga agtttttatt atttactttg
    70861 ggcctggaga caggaaaaat tatgctgaca ttcattgaac tccttattgg ggttgtggtt
    70921 attgtgggtg tggctcgcta catcattaaa gggtattccg ccactggtgt gttatttgtc
    70981 ggtggcctgt tattgctgat tatcagtgcc attatggggc acaaagtgtt accgtccagc
    71041 caggcttcaa caggctacag cgccacggat atcgttgaat acgttaaaat attactaatg
    71101 agccgcggcg gcgacctcgg catgatgatt atgatgctgt gtggctttgc cgcttacatg
    71161 acccatatcg gcgcgaatga tatggtggtc aagctggcgt caaaaccatt gcagtatatt
    71221 aactcccctt acctgctgat gattgccgcc tattttgttg cttgtctgat gtcactggcc
    71281 gtctcttccg caaccggtct gggggttttg ctgatggcaa ccctgtttcc ggtgatggta
    71341 aacgttggta tcagtcgtgg cgcagcagct gccatttgtg cctccccggc ggcgattatt
    71401 ctcgcaccga cttcagggga tgtggtgctg gcggcgcagg cttccgaaat gtcgctgatt
    71461 gacttcgcct tcaaaacgac gctgcctatc tcaattgctg caattatcgg catggcgatc
    71521 gcccacttct tctggcaacg ttatctggat aaaaaagagc acatctctca tgaaatgtta
    71581 gatgtcagtg aaatcaccac cactgccccc gcgttttatg ccattttgcc gttcacgccg
    71641 atcatcggtg tactgatttt tgacggtaaa tggggtccgc aattacacat catcactatt
    71701 ctggtgattt gtatgctgat tgcctccatt ctggagttcc tccgcagctt taatacccag
    71761 aaagttttct ctggtctgga agtggcttat cgcgggatgg cagatgcgtt tgctaacgtg
    71821 gtgatgctgc tggttgccgc tggggtattc gctcaggggc ttagcaccat cggctttatt
    71881 caaagtctga tttctatcgc tacctcgttt ggttcggcga gtatcatcct gatgctggta
    71941 ttggtgattc tgaccatgct ggcggcagtc acgaccggtt caggcaatgc gccgttttat
    72001 gcgtttgttg agatgatccc gaaactggcg cactcttccg gcattaaccc ggcgtatttg
    72061 actatcccga tgctgcaggc gtcaaacctg ggtcgtaccc tatcacccgt ttctggcgta
    72121 gtcgttgcgg ttgccgggat ggcgaaaatc tcaccatttg aagtcgtaaa acgcacctcg
    72181 gtgccggtgc ttgttgggct agtgattgtt atcgttgcta cagagctgat ggtgccagga
    72241 acggcagcag cggtcacagg caagtaaata gtaatgccgg aggcaagttt cctccggctt
    72301 tatcatgtca cccactgtgg tatatgcgtt gtggtctgcc aactttgccg tgaacaattt
    72361 cagcaataat cagatggcgg ctggcgcaat attcaagata acgcctggca gtggtgcggc
    72421 tgatggtcag tgcctgcgcc accgtttctg ccgtatgttg cacaccaggc tctttaaaca
    72481 gttttcgcac cgcgtttagc gtcaaggggt caatgccggt cggcagttcg tctttgggtt
    72541 caccgcgagc ataggcatta aacatctcat caatttgctt ctggctggcg ctatcaatac
    72601 tttccagcat atgtttacgc tggcggaaac gggttagcgt ttgccccagt gtattgattc
    72661 acttgaagta cgaaaaaacc gggagaacat tggattattc gggatctgat gggattagat
    72721 ttggtggggc ttgcaagcct gtagtacaaa ttttaggcgt taaccaatga aacgcgaaag
    72781 atagtaaaaa attgcttttg tttcattgaa aaaacgaaaa acaaaaacac tataaatcat
    72841 ttcaatcata gcttcaaaaa acgttcaaaa ccgataacaa ccaggctgtc atcaaatgac
    72901 tcatatcaca aatcagctca tgccgtttag gtatgttaca tgtgtgatta tgtgaggtga
    72961 agtatgtttt agctggctca tggttgttat acggcttttt tcgcatcttg tggttccaac
    73021 aaaggagcta caggctcttc ctgtttatga acaccataca ttgacaaaat ctcttggcga
    73081 gtttctggag gtaatttatg cagcgccaaa gctgtttgta gtgtttggcg atcaataccc
    73141 agcgttgcca atagggtgtc aacggagatg gaggggcgtt cttgcattaa tccggaaagc
    73201 ccatatttag ccatgtttag ctctatctct cggacttcat tttctcctag tttattgatt
    73261 aacagggaga acattttcaa tgtatcagca ttcatttctt cgatagcaat tttttgcata
    73321 gccggtattg ccgggatata ttcaacccca ggcccgcgac gcccttcttg tgaacgcttc
    73381 ggccatcctt ctttctttgc tcgtttatgc accccctgta ttgtcccagg cattccatcc
    73441 agttcagtaa tttgtttggg tgtcatccac tggtttatct catttgcgct tttcataaac
    73501 taagtttacc agatttatct atataaaaat caatggatta caaaataaac cacattaact
    73561 gatataaacc ggtttggttt atttggtaat gtgtaaaaca gataatcaag acgaattatt
    73621 tgttttggta accctttatc atacgcaata tggttggtgt gcgaaagatg agtaacgatt
    73681 ggcatcgggc cgacgtgatt gcggccctca aaaaaacagg aacccctctg gcaaaattgt
    73741 ccagacgttc aggttattca accgggacgc ttgcaaatgc attagtcagg ccatggccta
    73801 aaggtgaacg cattattgct gacgccttaa acatgaaacc tgaggaaatt tggcctagta
    73861 gatatctaag ggatcacaat gagagagtgg atatcagcaa ttgaatgcat gggtttatct
    73921 ggtttacctg gttcattaat gggggttcac aaaagagctc agagagaagg atggctcaag
    73981 agaagaagag aaggaattag aggggttgct tacgaatacc atatatcttc tcttcctaac
    74041 attgctcgag ctgaagtttt gctccaacaa ggaaaattta aaaccagtca gggatgtttt
    74101 gaaatcgccc gccccacgct ggaagctcat gattatgatc gtgaggcact atggagcaaa
    74161 tgggataaag ccagcgattc ccagcgcagc ctggctgaaa aatggttgcc ctcaattcag
    74221 gccacagacg aaatgctgaa ccaggggatt tcaacgaaaa cggcttttgc gaccgttgca
    74281 gggcattacc aggtcagcgc atccactttg cgggacaagt attaccaggt acagaagttt
    74341 gcgaagcctg actgggcggc tgcacttgtt gatggacgtg gagcatcccg tcgcaatgtt
    74401 cacaaaagtg aatttgacga ggatgcctgg cagtttctga ttgcagatta tctgcgaccg
    74461 gaaaaacccg ccttccgcaa atgttatgag cgtctggaac tggcagcccg cgagcatggc
    74521 tggagtattc cctcccgtgc cacggccttt cgccggattc agcaactgga cgaggcaatg
    74581 gttgttgcct gtcgtgaagg tgaacatgca ctgatgcatc tgataccggc acagcagcga
    74641 actgtggaac acctggacgc catgcagtgg atcaacggcg acggttatct gcataacgtc
    74701 tttgtacgct ggtttaacgg tgatgtgatc cgtccgaaaa catggttctg gcaggatgtg
    74761 aaaacccgaa aaattctggg ctggcgctgc gatgtgagcg agaacattga ttcaattcgc
    74821 ctctcgttca tggatgttgt gactcgctac agtatcccgg aggattttca catcaccatt
    74881 gataacaccc gtggtgctgc gaataaatgg ctgacgggag gcgcgcccaa tcgctaccgc
    74941 tttaaggtaa aagaggacga tccaaaagga ctgtttttac tgatgggtgc gaaaatgcac
    75001 tggacaagcg ttgttgccgg taaaggctgg ggccaggcaa aacctgttga acgtgctttc
    75061 ggtgttggtg ggcttgagga atacgttgat aagcatccgg cactggctgg cgcatatacg
    75121 gggccaaatc cgcaggcaaa acctgataac tatggcgacc acgctgttga tgcagagctg
    75181 tttctgaaaa cctttgccga aggtgtggcg atgttcaatg ccagaacagg ccgtgaaaca
    75241 gaaatgtgcg ggggcaaact ctcgtttgat gatgttttcg agcgtgaata cgccagaacg
    75301 attgtgcgta agccaaccga agaacaaaaa cggatgctgt tactgcctgc cgaggcggtg
    75361 aacgtttcac gcaaaggcga gtttacgctt aaagttggcg gctcccttaa aggcgcgaaa
    75421 aacgtttatt acaacatggc attaatgaat gccggagtga aaaaagttgt ggtcagattt
    75481 gatccacagc agttacacag cacggttt
//
LOCUS       DAJTRC010000004        72934 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.004, whole genome shotgun sequence.
ACCESSION   DAJTRC010000004 DAJTRC010000000
VERSION     DAJTRC010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 72934)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 72934)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72934
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.004"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO357_000301"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO357_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5885370.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO357_000302"
     CDS             complement(280..2394)
                     /gene="fusA"
                     /locus_tag="OO357_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020455498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HCR5885371.1"
                     /translation="MARTTPIARYRNIGISAHIDAGKTTTTERILFYTGVNHKIGEVH
                     DGAATMDWMEQEQERGITITSAATTAFWSGMAKQYEPHRINIIDTPGHVDFTIEVERS
                     MRVLDGAVMVYCAVGGVQPQSETVWRQANKYKVPRIAFVNKMDRVGANFLKVVNQIKT
                     RLGANPVPLQLAIGAEEHFTGVVDLVKMKAINWNDADQGVTFEYEDIPADMVELANEW
                     HQNLIESAAEASEELMEKYLGGEELTEAEIKGALRQRVLNNEIILVTCGSAFKNKGVQ
                     AMLDAVIDYLPSPVDVPAINGILDDGKDTPAERHASDDEPFSALAFKIATDPFVGNLT
                     FFRVYSGVVNSGDTVLNSVKAARERFGRIVQMHANKREEIKEVRAGDIAAAIGLKDVT
                     TGDTLCDPDAPIILERMEFPEPVISIAVEPKTKADQEKMGLALGRLAKEDPSFRVWTD
                     EESNQTIIAGMGELHLDIIVDRMKREFNVEANVGKPQVAYRETIRQKVTDVEGKHAKQ
                     SGGRGQYGHVVIDMYPLEPGSNPKGYEFINDIKGGVIPGEYIPAVDKGIQEQLKAGPL
                     AGYPVVDMGIRLHFGSYHDVDSSELAFKLAASIAFKEGFKKAKPVLLEPIMKVEVETP
                     EENTGDVIGDLSRRRGMLKGQESEVTGVKIHAEVPLSEMFGYATQLRSLTKGRASYTM
                     EFLKYDEAPSNVAQAVIEARGK"
     gene            complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO357_000303"
     CDS             complement(2491..2961)
                     /gene="rpsG"
                     /locus_tag="OO357_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015873274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HCR5885372.1"
                     /translation="MPRRRVIGQRKILPDPKFGSELLAKFVNILMVDGKKSTAESIVY
                     SALETLAQRSGKSELEAFEVALENVRPTVEVKSRRVGGSTYQVPVEVRPVRRNALAMR
                     WIVEAARKRGDKSMALRLANELSDAAENKGTAVKKREDVHRMAEANKAFAHYRW"
     gene            complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO357_000304"
     CDS             complement(3058..3432)
                     /gene="rpsL"
                     /locus_tag="OO357_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005306278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HCR5885373.1"
                     /translation="MATVNQLVRKPRARKVAKSNVPALEACPQKRGVCTRVYTTTPKK
                     PNSALRKVCRVRLTNGFEVTSYIGGEGHNLQEHSVILIRGGRVKDLPGVRYHTVRGAL
                     DCSGVKDRKQARSKYGVKRPKA"
     gene            complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO357_000305"
     CDS             complement(3558..3845)
                     /gene="tusB"
                     /locus_tag="OO357_000305"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusB"
                     /protein_id="HCR5885374.1"
                     /translation="MLHTLHRSPWLTDFAALLRLLSEGDELLLLQDGVTAAVDGNRYL
                     ESLRNAPIKVYALNEDLIARGLTGQILNDIIPIDYTDFVRLTVKHSSQMAW"
     gene            complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO357_000306"
     CDS             complement(3853..4212)
                     /gene="tusC"
                     /locus_tag="OO357_000306"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusC"
                     /protein_id="HCR5885375.1"
                     /translation="MKRIAFVFSTAPHGTAAGREGLDALLATSALTDDLAVFFIADGV
                     FQLLSGQKPDAVLARDYIATFKLLSLYDIEQCWVCAASLRERGLDPQTPFVVEATPLE
                     ADALRRELANYDVILRF"
     gene            complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO357_000307"
     CDS             complement(4212..4598)
                     /gene="tusD"
                     /locus_tag="OO357_000307"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase complex subunit TusD"
                     /protein_id="HCR5885376.1"
                     /translation="MRFAIVVTGPAYGTQQASSAFQFAQALIAEGHKLSSVFFYREGV
                     YNANQLTSPASDEFDLVRGWQQLNAQHGVALNICVAAALRRGIVDETEAGRLGLASSN
                     LQPGFTLSGLGALAEASLTCDRVVQF"
     gene            complement(4598..5320)
                     /locus_tag="OO357_000308"
     CDS             complement(4598..5320)
                     /locus_tag="OO357_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5885377.1"
                     /translation="MSRSLLTNETSELDLLDQRPFDQTDFDILKSYEAVVDGLAMLIG
                     SHCEIVLHSLQDLKCSAIRIANGEHTGRKIGSPITDLALRMLHDMTGADSSVSKCYFT
                     RAKSGVLMKSLTIAIRNREQRVIGLLCINMNLDVPFSQIMSTFVPPETPDVGSSVNFA
                     SSVEDLVTQTLEFTIEEVNADRNVSNNAKNRQIVLNLYEKGIFDIKDAINQVADRLNI
                     SKHTVYLYIRQFKSGDFQGQDK"
     gene            complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO357_000309"
     CDS             complement(5487..6299)
                     /gene="fkpA"
                     /locus_tag="OO357_000309"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5885378.1"
                     /translation="MKSLFKVTLLATTMAVALHAPITFAAEAAKPATTADSKASFKND
                     DQKSAYALGASLGRYMENSLKEQEKLGIKLDKDQLIAGVQDAFADKSKLSDQEIEQTL
                     QAFEARVKSSAQAKMEKDAADNEAKGKEYREKFAKEKGVKTSSTGLVYQVVEAGKGEA
                     PKDSDTVVVNYKGTLIDGKEFDNSYTRGEPLSFRLDGVIPGWTEGLKNIKKGGKIKLV
                     IPPELAYGKAGVPGIPPNSTLVFDVELLDVKPAPKADAKPEADAKAADSAKK"
     gene            6520..6738
                     /locus_tag="OO357_000310"
     CDS             6520..6738
                     /locus_tag="OO357_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein SlyX"
                     /protein_id="HCR5885379.1"
                     /translation="MQDLSLEARLAELESRLAFQEITIEELNVTVAAHEMEMAKLRDH
                     LRLLTEKLKASQPSNIASQAEETPPPHY"
     gene            complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO357_000311"
     CDS             complement(6787..7377)
                     /gene="slyD"
                     /locus_tag="OO357_000311"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5885380.1"
                     /translation="MKVAKDLVVSLAYQVRTEDGVLVDESPVSAPLDYLHGHGSLISG
                     LETALEGHEVGDKFDVAVGANDAYGQYDENLVQRVPKDVFMGVDELQVGMRFLAETDQ
                     GPVPVEITAVEDDHVVVDGNHMLAGQNLKFNVEVVAIREATEEELAHGHVHGAHDHHH
                     DHDHDGCCGGHGHDHGHEHGGEGCCGGKGNGGCGCH"
     gene            complement(7472..7672)
                     /locus_tag="OO357_000312"
     CDS             complement(7472..7672)
                     /locus_tag="OO357_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheV family putative metal-binding protein"
                     /protein_id="HCR5885381.1"
                     /translation="MAIRKRFIAGAKCPACQAQDSMAMWRENNIDIVECVKCGHQMRE
                     ADKEARDHVHKDEQVIGIFHPD"
     gene            complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO357_000313"
                     /pseudo
     CDS             complement(7682..9419)
                     /gene="kefB"
                     /locus_tag="OO357_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417809.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefB"
     gene            complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO357_000314"
     CDS             complement(9419..9970)
                     /gene="kefG"
                     /locus_tag="OO357_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     ancillary protein KefG"
                     /protein_id="HCR5885382.1"
                     /translation="MSQPAKVLLLYAHPESQDSVANRVLLKPATQLSNVTVHDLYAHY
                     PDFFIDIPREQALLREHEVIVFQHPLYTYSCPALLKEWLDRVLSRGFASGPGGNQLAG
                     KYWRNVITTGEPESAYRYDALNRYPMSDVLRPFELAAGMCRMHWLSPIIIYWARRQSA
                     KELASHARAYGDWLANPLSPGGR"
     gene            10101..12014
                     /locus_tag="OO357_000315"
     CDS             10101..12014
                     /locus_tag="OO357_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5885383.1"
                     /translation="MIVFSSLQIRRGVRVLLDNATATINPGQKVGLVGKNGCGKSTLL
                     ALLKNEISADGGSYTFPGSWQLAWVNQETPALPQAALEYVIDGDREYRQLEAQLHDAN
                     ERNDGHAIATIHSKLDAIDAWSIRSRAASLLHGLGFSNEQLERPVSDFSGGWRMRLNL
                     AQALICRSDLLLLDEPTNHLDLDAVIWLEKWLKSYQGTLILISHDRDFLDPIVDKIIH
                     IEQQSMFEYTGNYSSFEVQRATRLAQQQAMYESQQERVAHLQSYIDRFRAKATKAKQA
                     QSRIKMLERMELIAPAHVDNPFRFSFRAPESLPNPLLKMEKVSAGYGDRIILDSIKLN
                     LVPGSRIGLLGRNGAGKSTLIKLLAGELAPVSGEIGLAKGIKLGYFAQHQLEYLRADE
                     SPIQHLARLAPQELEQKLRDYLGGFGFLGDKVTEETRRFSGGEKARLVLALIVWQRPN
                     LLLLDEPTNHLDLDMRQALTEALIEFEGALVVVSHDRHLLRSTTDDLYLVHDRKVEPF
                     DGDLEDYQQWLSDVQKQENQTDEAPKENANSAQARKDQKRREAELRAQTQPLRKEIAR
                     LEKEMEKLNAQLAQAEEKLGDSELYDQSRKAELTACLQQQASAKSGLEECEMAWLEAQ
                     EQLEQMLLEGQSN"
     gene            12014..13036
                     /locus_tag="OO357_000316"
     CDS             12014..13036
                     /locus_tag="OO357_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5885384.1"
                     /translation="MAQITTTDANEFSSSAEFTPMRGFSNCHLQTMLPRLFRRQVKFT
                     PYWQRLELPDGDFVDLAWSEDPAQAKHKPRLVVFHGLEGSLNSPYAHGLVEAAQKRGW
                     LGVVMHFRGCSGEPNRMHRIYHSGETEDASWFLRWLQREFGHAPTAAVGYSLGGNMLA
                     CLLAKEGNDLPVDAAVIVSAPFMLEACSYHMEKGFSRVYQRYLLNLLKANAARKLAAY
                     PGTLPINLAQLKSVRRIREFDDLITARIHGYADAIDYYRQCSAMPMLNRIAKPTLIIH
                     AKDDPFMDHQVIPKPESLPSQVEYQLTEHGGHVGFIGGTLLHPQMWLESRIPDWLTTY
                     LEAKSC"
     gene            13030..13248
                     /locus_tag="OO357_000317"
     CDS             13030..13248
                     /locus_tag="OO357_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YheU family protein"
                     /protein_id="HCR5885385.1"
                     /translation="MLIPWQDLSPETLENLIESFVLREGTDYGEHERTLEQKVADVKR
                     QLQCGEAVLVWSELHETVNIMPRSQFRE"
     gene            13302..14171
                     /locus_tag="OO357_000318"
     CDS             13302..14171
                     /locus_tag="OO357_000318"
                     /EC_number="2.7.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribulokinase"
                     /protein_id="HCR5885386.1"
                     /translation="MSAKHPVIAVTGSSGAGTTTTSLAFRKIFAQLNLHAAEVEGDSF
                     HRYTRPEMDMAIRKARDAGRHISYFGPEANDFGLLEQTFIEYGQSGKGKSRKYLHTYD
                     EAVPWNQVPGTFTPWQPLPEPTDVLFYEGLHGGVVTPQHNVAQHVDLLVGVVPIVNLE
                     WIQKLIRDTSERGHSREAVMDSVVRSMEDYINYITPQFSRTHLNFQRVPTVDTSNPFA
                     AKGIPSLDESFVVIHFRNLEGIDFPWLLAMLQGSFISHINTLVVPGGKMGLAMELIML
                     PLVQRLMEGKKIE"
     gene            complement(14226..14630)
                     /locus_tag="OO357_000319"
     CDS             complement(14226..14630)
                     /locus_tag="OO357_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HCR5885387.1"
                     /translation="MQARVKWVEGLTFLGESASGHQILMDGNSGDKAPSPMEMVLMAA
                     GGCSAIDVVSILQKGRQDVVDCEVKLTSERREEAPRLFTHINLHFIVTGRDLKDAAVA
                     RAVDLSAEKYCSVALMLEKAVNITHSYEVVAA"
     gene            14932..15564
                     /gene="crp"
                     /locus_tag="OO357_000320"
     CDS             14932..15564
                     /gene="crp"
                     /locus_tag="OO357_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cAMP-activated global transcriptional regulator
                     CRP"
                     /protein_id="HCR5885388.1"
                     /translation="MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIV
                     KGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEGQERSAWVRAKTACEVAEISYKK
                     FRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
                     PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR"
     gene            15615..17705
                     /locus_tag="OO357_000321"
     CDS             15615..17705
                     /locus_tag="OO357_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709133.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HCR5885389.1"
                     /translation="MWRRLIYHPDINYALRQTLVLCLPVAVGLMLGELRFGLLFSLVP
                     ACCNIAGLDTPHKRFFKHLIIGASLFATCSLLTQLLLVKDVPLPFLLTGLTLVLGVTA
                     ELGPLHAKLLPASLLAAIFTLSLAGYMPVWEPLLIYALGTLWYGLFNWFWFWIWREQP
                     LRESLSLLYRELADYCEAKYSLLTQHTDPEKALPPLLVRQQKAVDLITQCYQQMHMLS
                     AQNNTDYKRMLRIFQEALDLQEHISVSLHQPEEVQKLVERSHAEEVIRWNAQTVAARL
                     RVLADDILYHRLPTRFTMEKQIGALEKIARQHPDNPVGQFCYWHFSRIARVLRTQKPL
                     YARDLLADKQRRMPLLPALKSYLSLKSPALRNAGRLSVMLSVASLMGTVLHLPKSYWI
                     LMTVLLVTQNGYGATRLRIVNRSVGTVVGLIIAGVALHFKIPESYTLTLMLITTLASY
                     LILRKNYGWATVGFTITAVYTLQLLWLNGEQYILPRLIDTIIGCLIAFGGTVWLWPQW
                     QSGLLRKNAHDALEAYQEAIRLILSEDPQPTPLAWQRMRVNQAHNTLYNSLNQAMQEP
                     AFNSHYLADMKLWVTHSQFIVEHINAMTTLAREHRALPPELAQEYLQSCEIAIQRCQQ
                     RLEYDEPGSSGDANIMDAPEMQPHEGAAGTLEQHLQRVIGHLNTMHTISSMAWRQRPH
                     HGIWLSRKLRDSKA"
     gene            complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO357_000322"
     CDS             complement(17772..18992)
                     /gene="argD/dapC"
                     /locus_tag="OO357_000322"
                     /EC_number="2.6.1.11"
                     /EC_number="2.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     acetylornithine/succinyldiaminopimelate transaminase"
                     /protein_id="HCR5885390.1"
                     /translation="MAIEQTAITRATFDEVILPIYAPAEFIPVKGQGSRIWDQQGKEY
                     VDFAGGIAVTALGHCHPALVNALKTQGETLWHISNVFTNEPALRLGRKLIEATFAERV
                     VFMNSGTEANETAFKLARHYACVRHSPFKTKIIAFHNAFHGRSLFTVSVGGQPKYSDG
                     FGPKPADIIHVPFNDLHAVKAVMDDHTCAVVVEPIQGEGGVTAATPEFLQGLRELCDQ
                     HQALLVFDEVQCGMGRTGDLFAYRYYGVTPDILTSAKALGGGFPISAMLTTAEIASAF
                     HPGSHGSTYGGNPLACAVAGAAFDIINTPEVLEGIQAKRQRFVDHLQKIDQQYDVFSD
                     IRGMGLLIGAELKPHYKGRARDFLYAGAEVGVMVLNAGPDVMRLAPSLVVEDADIDEG
                     MQRFAHAVAKVVGA"
     gene            complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO357_000323"
     CDS             complement(19078..19641)
                     /gene="pabA"
                     /locus_tag="OO357_000323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 2"
                     /protein_id="HCR5885391.1"
                     /translation="MILLIDNYDSFTWNLYQYFCELGADVLVKRNDALTLADIDALKP
                     QKIVISPGPCTPDEAGISLDVIRHYAGRLPILGVCLGHQAMAQAFGGKVVRAAKVMHG
                     KTSPITHSGEGVFRGLANPLTVTRYHSLVVEPDSLPACFDVTAWSETREIMGIRHRQW
                     DLEGVQFHPESILSEQGHQLLANFLHL"
     gene            complement(19673..20275)
                     /locus_tag="OO357_000324"
     CDS             complement(19673..20275)
                     /locus_tag="OO357_000324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative adenosine monophosphate-protein
                     transferase Fic"
                     /protein_id="HCR5885392.1"
                     /translation="MSDKFGEGRDLYLYPGLDIMRNRLNIRQQQRLEQAAYEMTALRA
                     ATIELGPLVRGLPHLRTIHRQLYQDIFDWAGQLREVDIYQGDTPFCHFAYIEKEGNAL
                     MQDLEEEGYLVGLEKAKFVERLAHYYCEINVLHPFRVGSGLAQRIFFEQLAIHAGYQL
                     SWQGIEKEAWNQANQSGAMGDLTALQMIFSKVVSEAGESE"
     gene            complement(20265..20432)
                     /locus_tag="OO357_000325"
     CDS             complement(20265..20432)
                     /locus_tag="OO357_000325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfG family protein"
                     /protein_id="HCR5885393.1"
                     /translation="MKKLTDKQKSRLWELQRNRNFQASRRLEGVEMPLVTLTAAEALA
                     RLEELRRHYER"
     gene            complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO357_000326"
                     /gene_synonym="rotA"
     CDS             complement(20537..21118)
                     /gene="ppiA"
                     /locus_tag="OO357_000326"
                     /gene_synonym="rotA"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase A"
                     /protein_id="HCR5885394.1"
                     /translation="MFKSTLAAMAAVFALSALSALSPAAMAAKGDPHVLLTTSAGNIE
                     LELDKQKAPVSVQNFVDYVNSGFYNNTTFHRVIPGFMIQGGGFTEQMQQKKPNPPIKN
                     EADNGLRNTRGTIAMARTADKDSATSQFFINVADNAFLDHGQRDFGYAVFGKVVKGMD
                     VADKISQVPTHDVGPYQNVPSKPVVILSAKVLP"
     gene            21389..22570
                     /gene="tsgA"
                     /locus_tag="OO357_000327"
     CDS             21389..22570
                     /gene="tsgA"
                     /locus_tag="OO357_000327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter TsgA"
                     /protein_id="HCR5885395.1"
                     /translation="MTNSNRIKLTWISFLSYALTGALVIVTGMVMGNIADYFNLPVSS
                     MSNTFTFLNAGILISIFLNAWLMEIVPLKTQLRFGFLLMVLAVAGLMFSHSLALFSTA
                     MFILGVVSGITMSIGTFLITQMYEGRQRGSRLLFTDSFFSMAGMIFPMIAAFLLARSI
                     EWYWVYACIGLVYVAIFILTFGCEFPALGKRAPKTDAPVEKEKWGIGVLFLSVAALCY
                     ILGQLGFISWVPEYAKGLGMSLNDAGTLVSNFWMSYMVGMWAFSFILRFFDLQRILTV
                     LAGLAAILMYVFNTGTPVHMAWSILALGFFSSAIYTTIITLGSQQTKVPSPKLVNFVL
                     TCGTIGTMLTFVVTGTIVEHSGPQAALLTANGLYAVVFVMCFLLGFVSRHRQHNTLTS
                     H"
     gene            22832..25375
                     /gene="nirB"
                     /locus_tag="OO357_000328"
     CDS             22832..25375
                     /gene="nirB"
                     /locus_tag="OO357_000328"
                     /EC_number="1.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-nitrite reductase large subunit"
                     /protein_id="HCR5885396.1"
                     /translation="MSKVRLAIIGNGMVGHRFIEDLLDKSDAANFDITVFCEEPRIAY
                     DRVHLSSYFSHHTAEELSLVREGFYEKHGIKVLVGERAITINRQEKVIHSSAGRTVFY
                     DKLIMATGSYPWIPPIKGSDTQDCFVYRTIEDLNAIESCARRSKRGAVVGGGLLGLEA
                     AGALKNLGIETHVIEFAPMLMAEQLDQMGGEQLRRKIESMGVRVHTSKNTLEIVQEGV
                     EARKTMRFADGSELEVDFIVFSTGIRPRDKLATQCGLDVAPRGGIVINDSCQTSDPDI
                     YAIGECASWNNRVFGLVAPGYKMAQVAVDHILGSENAFEGADLSAKLKLLGVDVGGIG
                     DAHGRTPGARSYVYLDESKEIYKRLIVSEDNKTLLGAVLVGDTSDYGNLLQLVLNAIE
                     LPENPDSLILPAHSGSGKPSIGVDKLPDSAQICSCFDVTKGDLIAAINKGCHTVAALK
                     AETKAGTGCGGCIPLVTQVLNAELAKQGIEVNNNLCEHFAYSRQELFHLIRVEGIKTF
                     EELLAKHGKGYGCEVCKPTVGSLLASCWNEYILKPEHTPLQDSNDNFLANIQKDGTYS
                     VIPRSPGGEITPEGLMAVGRIAREFNLYTKITGSQRLAMFGAQKDDLPEIWRQLIEAG
                     FETGHAYAKALRMAKTCVGSTWCRYGVGDSVGLGVELENRYKGIRTPHKMKFGVSGCT
                     RECSEAQGKDVGIIATEKGWNLYVCGNGGMKPRHADLLAADIDRETLIKYLDRFMMFY
                     IRTADKLTRTAPWLENLEGGIDYLKAVIIDDKLGLNAHLEEEMARLREAVVCEWTETV
                     NTPSAQTRFKHFINSDKRDPNVQMVPEREQHRPATPYERIPVTLVEDNA"
     gene            25372..25698
                     /gene="nirD"
                     /locus_tag="OO357_000329"
     CDS             25372..25698
                     /gene="nirD"
                     /locus_tag="OO357_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HCR5885397.1"
                     /translation="MSQWKDICKIDDILPETGVCALLGDKQVAIFRPYHSDQVFAISN
                     IDPFFESSVLSRGLIAEHQGELWVASPLKKQRFRLSDGLCMEDEQFSVKHYEARVKDG
                     VVQLRG"
     gene            25824..26630
                     /gene="nirC"
                     /locus_tag="OO357_000330"
     CDS             25824..26630
                     /gene="nirC"
                     /locus_tag="OO357_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite transporter NirC"
                     /protein_id="HCR5885398.1"
                     /translation="MFTDTINKCAANAARIARLSANNPLGFWVSSAMAGAYVGLGIIL
                     IFTLGNLLDPSVRPLVMGATFGIALTLVIIAGSELFTGHTMFLTFGVKAGSISHGQMW
                     AILPQTWLGNLVGSVFVAMLYSWGGGSLLPVDTSIVHSVALAKTTAPAMVLFFKGALC
                     NWLVCLAIWMALRTEGAAKFIAIWWCLLAFIASGYEHSIANMTLFALSWFGNHSEAYT
                     LAGIGHNLLWVTLGNTLSGAVFMGLGYWYATPKANRPVADKFNQTETAAG"
     gene            26649..28022
                     /gene="cobA"
                     /locus_tag="OO357_000331"
     CDS             26649..28022
                     /gene="cobA"
                     /locus_tag="OO357_000331"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5885399.1"
                     /translation="MDHLPIFCQLRDRDCLIVGGGDVAERKARLLLDAGARLTVNALA
                     FIPQFTAWADAGMLTLVEGPFDESLLDTCWLAIAATDDDTLNQRVSEAAEARRIFCNV
                     VDAPKAASFIMPSIIDRSPLMVAVSSGGTSPVLARLLREKLESLLPLHLGQVAKYAGQ
                     LRGRVKQQFATMSERRRFWEKLFVNDRLAQSLANNDQKAITETTEQLINEPLDHRGEV
                     VLVGAGPGDAGLLTLKGLQQIQQADVVVYDRLVSDDIMNLVRRDADRVFVGKRAGYHC
                     VPQEEINQILLREAQKGKRVVRLKGGDPFIFGRGGEELETLCNAGIPFSVVPGITAAS
                     GCSAYSGIPLTHRDYAQSVRLITGHLKTGGELDWENLAAEKQTLVFYMGLNQAATIQQ
                     KLIEHGMPGEMPVAIVENGTAVTQRVIDGTLTQLGELAQQMNSPSLIIIGRVVGLRDK
                     LNWFSNH"
     gene            28269..28436
                     /locus_tag="OO357_000332"
     CDS             28269..28436
                     /locus_tag="OO357_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfL family protein"
                     /protein_id="HCR5885400.1"
                     /translation="MNKFIKVALVGAVLATLTACTGHIENRDKNCSYDYLLHPAISIS
                     KIIGGCGPTAQ"
     gene            28731..30068
                     /gene="frlA"
                     /locus_tag="OO357_000333"
                     /pseudo
     CDS             28731..30068
                     /gene="frlA"
                     /locus_tag="OO357_000333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417829.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine/psicoselysine transporter"
     gene            30089..31111
                     /gene="frlB"
                     /locus_tag="OO357_000334"
     CDS             30089..31111
                     /gene="frlB"
                     /locus_tag="OO357_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417830.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-phosphate deglycase"
                     /protein_id="HCR5885401.1"
                     /translation="MLDIDKSTVDFLVTENMVQEVEKVLSHDVPLVHAIVEEMVKRDI
                     DRIYFVACGSPLNAAQTAKHLADRFSDLQVYAISGWEFCDNTPYRLDDRCAVIGVSDY
                     GKTEEVIKALELGRACGALTAAFTKRADSPITSAAEFSIDYQADCIWEIHLLLCYSVV
                     LEMITRLAPNAEIGKIKNDLKQLPNALGHLVRTWEEKGRQLGELASQWPMIYTVAAGP
                     LRPLGYKEGIVTLMEFTWTHGCVIESGEFRHGPLEIVEPGVPFLFLLGNDESRHTTER
                     AINFVKQRTDNVIVIDYAEISQGLHPWLAPFLMFVPMEWLCYYLSIYKDHNPDERRYY
                     GGLVEY"
     gene            31161..31991
                     /gene="frlC"
                     /locus_tag="OO357_000335"
     CDS             31161..31991
                     /gene="frlC"
                     /locus_tag="OO357_000335"
                     /EC_number="5.1.3.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709146.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 3-epimerase"
                     /protein_id="HCR5885402.1"
                     /translation="MKTGMFTCGHQRLPIEHAFRDASELGYDGIEIWGGRPHAFAPDL
                     KAGGIKQIKALAQTYQMPIIGYTPETNGYPYNMMLGDEHMRRESLDMIKLAIDMAKEM
                     NAGYTLISAAHAGYLTPPNVIWGRLAENLSELCEYAENIGMDLILEPLTPYESNVVCN
                     ANDVLHALALVPSPRLFSMVDICAPYVQAEPVMSYFDKLGDKLRHLHIVDSDGASDTH
                     YIPGEGKMPLRELMRDIIDRGYEGYSTVELVTMYMNEPRLYARQALERFRALLPEDER
                     "
     gene            31988..32773
                     /gene="frlD"
                     /locus_tag="OO357_000336"
     CDS             31988..32773
                     /gene="frlD"
                     /locus_tag="OO357_000336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HCR5885403.1"
                     /translation="MKTLATIGDNCVDIYPQLNKAFSGGNAVNVAVYCTRYGIQPGCI
                     TWVGDDDYGTKLKQDLARMGVDISHVHTKHGVTAQTQVELHDNDRVFGDYTEGVMADF
                     ALSEEDYAWLAQYDIVHAAIWGHAEDAFPQLHAAGKLTAFDFSDKWDSPLWQTLVPHL
                     DFAFASAPQEDEALRLKMKAIVARGAGTVIVTLGENGSIAWDGAQFWRQAPEPVTVID
                     TMGAGDSFIAGFLCGWSAGMTLPQAMAQGTACAAKTIQYHGAW"
     gene            32873..33604
                     /gene="phoB"
                     /locus_tag="OO357_000337"
     CDS             32873..33604
                     /gene="phoB"
                     /locus_tag="OO357_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417834.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator PhoB"
                     /protein_id="HCR5885404.1"
                     /translation="MSATDRYSHQLLYATVRQRLLDDIAQGVYQAGQQIPTENELCTQ
                     YNVSRITIRKAISDLVADGVLIRWQGKGTFVQSQKVENALLTVSGFTDFGVSQGKSTK
                     EKVIEQERVSAAPFCEKLNIPGNSEVFHLCRVMYLDKEPLFIDSSWIPLSRYPDFDEI
                     YVEGSSTYQLFQERFDTRVVSDKKTIDIFAATRPQAKWLKCELGEPLFRISKIAFDQN
                     DKPVHVSELFCRANRITLTIDNKRH"
     gene            complement(33764..34849)
                     /locus_tag="OO357_000338"
     CDS             complement(33764..34849)
                     /locus_tag="OO357_000338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HCR5885405.1"
                     /translation="MKTFPLQSLTLAEAQQKQFALVDTICRHFPGSEFLAGGDLGLTP
                     SLNQPRITQRVEQVLADAFHAQAAALVQGAGTGAIRAGLAALLKPGQRLLVHDAPVYP
                     TTRVIIEQMGLTLITADFNDLSALKQVVDEQQPDAALVQHTRQQPQDSYVLADVLATL
                     RAAGVPALTDDNYAVMKVARIGCECGANVSTFSCFKLFGPEGVGAVVGDADVISRIRA
                     TLYSGGSQIQGSQALEVLRGLVFAPVMHAVQAGVSERLLALLNGGAVAEVKSAVIANA
                     QSKVLIVEFHQLIAARVLEEAQKRGALPYPVGAESKYEIPPLFYRLSGTFRQANPQLE
                     HCAIRINPNRSGEETVLRILRESIASI"
     gene            complement(34861..36165)
                     /locus_tag="OO357_000339"
     CDS             complement(34861..36165)
                     /locus_tag="OO357_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfT family protein"
                     /protein_id="HCR5885406.1"
                     /translation="MDLYIQIIVVACLTGMTSLLEHRSAAVFHDGIHPILPQLIEGYM
                     NRREAGSIAFGLSIGFVASVGISFTLKTGLLNAWLLFLPTDILGVLAINSLMAFGLGA
                     IWGVLILTCLLPVNQLLTALPVDVLGSLGELSSPVVSAFALFPLVAIFYQFGWKQSLV
                     AAVVVLMTRVVVVRYFPHLNPESIEIFIGMVMLLGIAITHDLRHRDENDIDASGLSVF
                     EERTSRIIKNLPYIAIVGALIAAVASMKIFAGSEVSIFTLEKAYSAGVTPEQSQTLIN
                     QAALAEFMRGLGFVPLIATTALATGVYAVAGFTFVYAVGYLSPNPMVAAVLGAVVISA
                     EVLLLRSIGKWLGRYPSVRNASDNIRNAMNMLMEVALLVGSIFAAIKMAGYTGFSIAV
                     AIYFLNESLGRPVQKMAAPVVAVMITGILLNVLYWLGLFVPA"
     gene            complement(36177..36530)
                     /locus_tag="OO357_000340"
     CDS             complement(36177..36530)
                     /locus_tag="OO357_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2620 domain-containing protein"
                     /protein_id="HCR5885407.1"
                     /translation="MKKIGVAGLQREQIKKTIEATAPGCFEVFIHNDMEAAMKVKSGQ
                     LDYYIGACNTGAGAALSIAIAVIGYNKSCTIAKPGIKAKDEHIAKMIAEGKVAFGLSV
                     EHVEHAIPMLINHLK"
     gene            complement(36541..37419)
                     /locus_tag="OO357_000341"
     CDS             complement(36541..37419)
                     /locus_tag="OO357_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotriesterase-related protein"
                     /protein_id="HCR5885408.1"
                     /translation="MSFDPTGYTLAHEHLHIDLSGFKNNVDCRLDQYAFICQEMNDLM
                     TRGVRNVIEMTNRYMGRNAQFMLDVMRETGINVVACTGYYQDAFFPEHVVTRSVQELA
                     QEMVDEIEQGIDGTELKAGIIAEIGSSEGKITPLEEKVFIAAALAHNQTGRPISTHTS
                     FSTMGLEQLALLQAHGVDLSRVTVGHCDLKDNLDNILKMIDLGAYVQFDTIGKNSYYP
                     DEKRIAMLHALRDRGLLNRVMLSMDITRRSHLKANGGYGYDYLLTTFIPQLRQSGFSQ
                     ADVDVMLRENPSQFFQ"
     gene            complement(37416..38642)
                     /locus_tag="OO357_000342"
     CDS             complement(37416..38642)
                     /locus_tag="OO357_000342"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5885409.1"
                     /translation="MARFVVLVIDSFGVGAMKDVTLVRPQDAGANTCGHILSQLPHLQ
                     LPTLEKLGLINTLGYAPGDMQPSDSATWGVAELQHEGGDTFMGHQEILGTRPLPPLRM
                     PFRDVIDRVEQALVSAGWQVERRGDDLQFLSVNQAVAIGDNLEADLGQVYNITANLSV
                     ISFDDAIKIGRIVRDQVQVGRVITFGGLLTNSQRILDAAESKEGRFIGINAPRSGAYD
                     NGFQVVHMGYGVDEKVQVPQKLYEAGVPTVLVGKVADIVSNPYGVSWQNLVDSQRIMD
                     ITLDEFNTHPTAFICINIQETDLAGHAEDVARYAERLQVVDRNLARLVEAMQPDDCLV
                     VMADHGNDPTIGHSHHTREVVPVLVYQQGLVATQLGVRTTLSDVGATVCEFFRAPPPQ
                     NGRSFLSSLRFAGDTL"
     gene            complement(38643..39806)
                     /locus_tag="OO357_000343"
     CDS             complement(38643..39806)
                     /locus_tag="OO357_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhfX family PLP-dependent enzyme"
                     /protein_id="HCR5885410.1"
                     /translation="MFVEALKRQNPALISAALSLWQQGKIAPDSWVIDVDQILENGKR
                     LIETARLYGIELYLMTKQFGRNPWLAEKLLALGYSGIVAVDYKEARVMRRAGLPVAHQ
                     GHLVQIPCHQVADAVEQGTDIITVFTLDKAREVSAAAVKAGRVQPVLLKVYSDDDFLY
                     PGQESGFVQHSLHEVIAEIKKLPGLHLAGLTHFPCLLWDEAAGKVLPTPNLHTLVQAR
                     DQLAKSGIAIEQLNVPSATSCTSLPLLAEYGATHAEPGHALTGTIPANQQGDQPERIA
                     MLWLSEISHHFRGDSYCYGGGYYRRGHAQHALVFTPENQKITETNLKTVDDSSIDYTL
                     PLAGEYPVSSAVVLCFRTQIFVTRSDVVLVSGIQRGEPEIVGRYDSLGNPLEA"
     gene            complement(39890..40252)
                     /locus_tag="OO357_000344"
     CDS             complement(39890..40252)
                     /locus_tag="OO357_000344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HCR5885411.1"
                     /translation="METRLNLLCEAGVIDKDVCKGMMQVVNVLEKECHLPVRSEQGTM
                     AMTHMASALMRSRRGEEIEPLDNELLAELAQSSHWQAVVQLHQELLKEFALEVNPCEE
                     GYFLANLYGLWMAANEEV"
     gene            complement(40269..41174)
                     /locus_tag="OO357_000345"
     CDS             complement(40269..41174)
                     /locus_tag="OO357_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709156.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885412.1"
                     /translation="MRRTFIKKEGVVITTLARYLLGEKCGNRLKTIDELATECRSSVG
                     LTQAALKTLESSGAIRIERRGRNGSYLVEMDNKALLSHVDINNVVCAMPLPYTRLYEG
                     LASGLKAQFDGIPFYYAHMRGADIRVECLLNGVYDMAVVSRLAAESYLSQNNLCIALE
                     LGPHTYVGEHQLICRKGESGNVKRVGLDSRSADQKIMTDVFFGDSDVERVDLSYHESL
                     QRIVKGDVDAVIWNVVAENELTMLGLEATPLTDDPRFLQATEAVVLTRVDDYPMQQLL
                     RAVVDKHALLAHQQRVVSGEQEPSY"
     gene            complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OO357_000346"
     CDS             complement(41444..42448)
                     /gene="trpS"
                     /locus_tag="OO357_000346"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HCR5885413.1"
                     /translation="MTKPIVFSGAQPSGELTIGNYMGALRQWVNMQDDYHCIYCIVDQ
                     HAITVRQDAQKLRKATLDTLALYLACGIDPEKSTIFVQSHVPEHAQLGWALNCYTYFG
                     ELSRMTQFKDKSARYAENINAGLFGYPVLMAADILLYQTNLVPVGEDQKQHLELSRDI
                     AQRFNALYGEIFKVPEPFIPKSGARVMSLLEPTKKMSKSDDNRNNVIGLLEDPKSVVK
                     KIKRAVTDSDEPPVVRYDVQNKAGVSNLLDILSAVTGQSIPELEKQFEGKMYGHLKGE
                     VADAVSGMLTELQERYHRFRNDEAFLQQVMKDGAEKASVHASRTLKAVYEAIGFVAKP
                     "
     gene            complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OO357_000347"
     CDS             complement(42441..43199)
                     /gene="gph"
                     /locus_tag="OO357_000347"
                     /EC_number="3.1.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycolate phosphatase"
                     /protein_id="HCR5885414.1"
                     /translation="MNKFEDIRGVAFDLDGTLVDSAPGLAAAVDMALYALELPVAGEE
                     RVITWIGNGADVLMERALTWARQERATLRKTMGKPPVDDDIPAEEQVRILRKLFDRYY
                     GEVAEEGTFLFPHVADTLGALQAKGLPLGLVTNKPTPFVAPLLEALDIAKYFSVVIGG
                     DDVQNKKPHPDPLLLVAERMGIAPQQMLFVGDSRNDIQAAKAAGCPSVGLTYGYNYGE
                     AIDLSQPDVIYQSINDLLPALGLPHSENQESKND"
     gene            complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OO357_000348"
     CDS             complement(43192..43869)
                     /gene="rpe"
                     /locus_tag="OO357_000348"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HCR5885415.1"
                     /translation="MKQYLIAPSILSADFARLGEDTAKALAAGADVVHFDVMDNHYVP
                     NLTIGPMVLKSLRNYGITAPIDVHLMVKPVDRIVPDFAAAGASIITFHPEASEHVDRT
                     LQLIKENGCKAGLVFNPATPLSYLDYVMDKLDVILLMSVNPGFGGQSFIPQTLDKLRE
                     VRRRIDESGFDIRLEVDGGVKVNNIGEIAAAGADMFVAGSAIFDQPDYKKVIDEMRSE
                     LAKVSHE"
     gene            complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OO357_000349"
     CDS             complement(43887..44723)
                     /gene="dam"
                     /locus_tag="OO357_000349"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5885416.1"
                     /translation="MKKNRAFLKWAGGKYPLLDDIKRHLPKGECLVEPFVGAGSVFLN
                     TDFSRYILADINSDLISLYNIVKMRTDEYVQAARELFVPETNCAEVYYQFREEFNKSQ
                     DPFRRAVLFLYLNRYGYNGLCRYNLRGEFNVPFGRYKKPYFPEAELYHFAEKAQNAFF
                     YCESYADSMERADDASVVYCDPPYAPLSATANFTAYHTNSFTLEQQAHLAEIAEGLVE
                     RHIPVLISNHDTMLTREWYQRAKLHVVKVRRSISSNGGTRKKVDELLALYKPGVVSPA
                     KK"
     gene            complement(44830..46122)
                     /gene="damX"
                     /locus_tag="OO357_000350"
     CDS             complement(44830..46122)
                     /gene="damX"
                     /locus_tag="OO357_000350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DamX"
                     /protein_id="HCR5885417.1"
                     /translation="MDEFKPEDELKPDPSDRRTGRSRQSSERSERTERGEPQINFDDI
                     ELDDTDDRRPTRAQKERNEEPEIEEEIDESEDETVDEERVERRPRKRKKAASKPASRQ
                     YMMMGVGILVLLLLIIGIGSALKAPSTTSSDQTASGEKSIDLAGNATDQANGVQPAPG
                     TTSAENTQQDVSLPPISSTPTQGQTPVATDGQQRVEVQGDLNNALTQPQNQQQLNNVA
                     VNSTLPTEPATVAPVRNGNASRDTAKTQTAERPSTTRPVRQQAVIEPKKPQATVKTEP
                     KPVAQTPKRTEPEPAAPVASTKAPAATSTPAPKETATTAPVQTASPAQTTATPAAGGK
                     TAGNVGSLKSAPSSHYTLQLSSSSNYDNLNGWAKKENLKNYVVYETTRNGQPWYVLVS
                     GVYASKEEAKKAVSTLPADVQAKNPWAKPLRQVQADLK"
     gene            complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="OO357_000351"
     CDS             complement(46214..47302)
                     /gene="aroB"
                     /locus_tag="OO357_000351"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HCR5885418.1"
                     /translation="MERIVVTLGERSYPITIASGLFNEPASFLPLKSGEQVMLVTSET
                     LAPLYLDKVRGVLEQAGVNVDSVILPDGEQYKSLAVLDTVFTALLQKPHGRDTTLVAL
                     GGGVVGDLTGFAAASYQRGVRFIQVPTTLLSQVDSSVGGKTAVNHPLGKNMIGAFYQP
                     ASVVVDLDCLKTLPPRELASGLAEVIKYGIILDGAFFNWLEENLDALLRLDGPAMAYC
                     IRRCCELKAEVVAADERETGLRALLNLGHTFGHAIEAEMGYGNWLHGEAVAAGMVMAA
                     RTSERLGQFSSAETQRIITLLTRAGLPVNGPREMSAQAYLPHMLRDKKVLAGEMRLIL
                     PLAIGKSEVRSGVSHELVLNAIADCQSA"
     gene            complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="OO357_000352"
     CDS             complement(47359..47880)
                     /gene="aroK"
                     /locus_tag="OO357_000352"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004709337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroK"
                     /protein_id="HCR5885419.1"
                     /translation="MAEKRNIFLVGPMGAGKSTIGRQLAQQLNMEFYDSDQEIEKRTG
                     ADVGWVFDLEGEEGFRDREEKVINELTEKQGIVLATGGGSVKSRETRNRLSARGVVVY
                     LETTIEKQLARTQRDKKRPLLHVETPPREVLEALANERNPLYEEIADVTIRTDDQSAK
                     VVANQIIHMLESN"
     gene            complement(48284..49522)
                     /gene="hofQ"
                     /locus_tag="OO357_000353"
     CDS             complement(48284..49522)
                     /gene="hofQ"
                     /locus_tag="OO357_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA uptake porin HofQ"
                     /protein_id="HCR5885420.1"
                     /translation="MKQWIAALLLMLIPGVQAAKPQKVTLMVDDVPVAQVLQALAEQE
                     KLNLVVSPDVSGTVSLHLTDVPWKQALQTVVKSAGLITRQEGNILSVHSIAWQNDNIA
                     RQEAEQARAQANLPLENRSITLQYADAGELAKAGEKLLSAKGSMTVDKRTNRLLLRDN
                     KTALSALEQWVAQMDLPVGQVELSAHIVTINEKSLRELGVKWTLADAQHAGGVGQVTT
                     LGSDLSVATATTHVGFNIGRINGRLLDLELSALEQKQQLDIIASPRLLASHLQPASIK
                     QESEIPYQVSSGESGATSVEFKEAVLGMEVTPTVLQKGRIRLKLHISQNVPGQVLQQA
                     DGEVLAIDKQEIETQVEVKSGETLALGGIFTRKNKSGQDSVPLLGDIPWFGQLFRHDG
                     KEDERRELVVFITPRLVSSE"
     gene            complement(49434..49840)
                     /gene="hofP"
                     /locus_tag="OO357_000354"
                     /pseudo
     CDS             complement(49434..49840)
                     /gene="hofP"
                     /locus_tag="OO357_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417851.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofP"
     gene            complement(49830..50270)
                     /gene="hofO"
                     /locus_tag="OO357_000355"
     CDS             complement(49830..50270)
                     /gene="hofO"
                     /locus_tag="OO357_000355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofO"
                     /protein_id="HCR5885421.1"
                     /translation="MNMFFDWWFATSPRLRQFCWAFWLLMLVTLIFLSSTHHEERDAL
                     IRLRASHHQQWAALYRLVDTTPFSEEKTLPFSPLDFQLSGAQLVSWHPSAQGGELALK
                     TLWEAVPSAFTRLAERNVSVSRFSLSVEGDDLLFTLQLEMPHEG"
     gene            complement(50254..50793)
                     /gene="hofN"
                     /locus_tag="OO357_000356"
     CDS             complement(50254..50793)
                     /gene="hofN"
                     /locus_tag="OO357_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofN"
                     /protein_id="HCR5885422.1"
                     /translation="MNPPINFLPWRQQRRTAFLRFWLLMFVAPLLLAVGITLILRLTG
                     SAEARIDAVLLQAEQQLARSLQITKPRLLEQQQLREQRSQRQRQRQFTRDWQSALEAL
                     AALLPEHAWLTTISWQQGTLEIKGLTTSITALNALETSLRQDASFHLNQRGATQQDAQ
                     GRWQFEYQLTRKVSDEHVL"
     gene            complement(50793..51572)
                     /gene="hofM"
                     /locus_tag="OO357_000357"
     CDS             complement(50793..51572)
                     /gene="hofM"
                     /locus_tag="OO357_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein HofM"
                     /protein_id="HCR5885423.1"
                     /translation="MTFKIWQIGLHLQQQEAAAVAIVRGAKECFLQRWWRLPLAHDII
                     KDGRIVDAQQLAKTLLPWSRELPQRHHIMLAFPASRTLQRSFPRPSMSLGEREQTAWL
                     SGTMARELDMDPDSLRFDYSEDSLSPAYNVTAAQSKELATLLTLAERLRVHVSAITPD
                     ASALQRFLPFLPSHQQCLAWRDNEQWLWATRYSWGRKLAVGMTSAKELAAALSVDPDS
                     VAICGEGGFDPWEAVSVRQPPLPPPGGDFAIALGLALGKAY"
     gene            51692..54244
                     /gene="mrcA"
                     /locus_tag="OO357_000358"
     CDS             51692..54244
                     /gene="mrcA"
                     /locus_tag="OO357_000358"
                     /EC_number="2.4.1.129"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase/peptidoglycan
                     DD-transpeptidase MrcA"
                     /protein_id="HCR5885424.1"
                     /translation="MKFVKYFLILAVCCILLGAGSIYGLYRYIEPQLPDVVTLKDVRL
                     QIPMQIYSADGELIAQYGEKRRIPVTLDQIPPEMVKAFIATEDSRFYEHHGVDPVGIF
                     RAASVALFSGHASQGASTITQQLARNFFLSPERTMMRKIKEVFLAIRIEQLLTKDEIL
                     ELYLNKIYLGYRAYGVGAAAQVYFGKTVDQLTLNEMAVIAGLPKAPSTFNPLYSMDRA
                     VARRNVVLSRMLDEGYITQQQFDQTRTEAINANYHAPEIAFSAPYLSEMVRQEMYNRY
                     GESAYEDGYRIYTTITRKVQQAAQQAVRNNVLDYDMRHGYRGPANVLWKVGESAWDNN
                     KITDTLKALPTYGPLLPAAVTSANPQGATAMLADGSTVALSMEGVRWARPYRSDTQQG
                     PTPRKVTDVLQTGQQIWVRQVGDAWWLAQVPEVNSALVSINPQNGAVMALVGGFDFNQ
                     SKFNRATQALRQVGSNIKPFLYTAAMDKGLTLASMLNDVPISRWDAGAGSDWQPKNSP
                     PQYAGPIRLRQGLGQSKNVVMVRAMRAMGVDYAAEYLQRFGFPAQNIVHTESLALGSA
                     SFTPMQVARGYAVMANGGFLVDPWFISKIENDQGGVIFEAKPKVACPECDIPVIYGDT
                     QKSNVLENNDVEDVAISREQQNVSVPMPQLEQANQALVAKTGAQEYAPHVINTPLAFL
                     IKSALNTNIFGEPGWQGTGWRAGRDLQRRDIGGKTGTTNSSKDAWFSGYGPGVVTSVW
                     IGFDDHRRNLGHTTASGAIKDQISGYEGGAKSAQPAWDAYMKAVLEGVPEQPLTPPPG
                     IVTVNIDRSTGQLANGGNSREEYFIEGTQPTQQAVHEVGTTIIDNGEAQELF"
     gene            complement(54409..54969)
                     /gene="nudE"
                     /locus_tag="OO357_000359"
     CDS             complement(54409..54969)
                     /gene="nudE"
                     /locus_tag="OO357_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP compounds hydrolase NudE"
                     /protein_id="HCR5885425.1"
                     /translation="MNKSLQKPTILNVETVARSRLFTVESVDLEFSNGVRRVYERMRP
                     TNREAVMIVPIVDDHLILIREYAVGTESYELGFSKGLIDPGESVYEAANRELKEEVGF
                     GANDLTFLKKLSMAPSYFSSKMNIVVAQDLYPESLEGDEPEPLPQVRWPLAHMMDLLE
                     DPDFNEARNVSALFLVREWLKGQGRV"
     gene            55289..57424
                     /gene="igaA"
                     /locus_tag="OO357_000360"
     CDS             55289..57424
                     /gene="igaA"
                     /locus_tag="OO357_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intracellular growth attenuator protein IgaA"
                     /protein_id="HCR5885426.1"
                     /translation="MSTIVIFLAALLACSLLAGWLIKVRSRRRQLPWTNAFADAQTRK
                     LTPEERSAVENYLESLTQVLQVPGPTEASAAPISLALNAESNNVMMLTHAITRYGIST
                     DDPNKWRYYLDSVEVHLPPFWEQYINDENTVELIHTDSLPLVISLNGHTLQEYMQETR
                     GYALQPVPSTQASIRGEESEQIELLNIRKETHEEYALSRPRGLREALLIVASFLMFFF
                     CLITPDVFVPWLAGGALLLLGAGLWGLFAPPAKSSLREIHCLRGTPRRWGLFGENDQE
                     QINNISLGIIDLVYPAHWQPYIAQDLGQQTDIDIYLDRHVVRQGRYLSLHDEVKNFPL
                     QHWLRSTIIAAGSLLVLFMLLFWIPLDMPLKFTLSWMKGAQTIEATSVKQLADAGVRV
                     GDTLRISGTGMCNIRTSGTWSAKTNSPFLPFDCSQIIWNDARSLPLPESELVNKATAL
                     TEAVNRQLHPKPEDESRVSASLRSAIQKSGMVLLDDFGDIVLKTADLCSAKDDCVRLK
                     NALVNLGNSKDWDALVKRANAGKLDGVNVLLRPVSAESLDNLVATSTAPFITHETARA
                     AQSLNSPAPGGFLIVSDEGSDFVDQPWPSASLYDYPPQEQWNAFQKLAQMLMHTPFNA
                     EGIVTKIFTDANGTQHIGLHPIPDRSGLWRYLSTTLLLLTMLGSAIYNGVQAWRRYQR
                     HRTRMMEIQAYYESCLNPQLITPSESLIE"
     gene            57489..58157
                     /gene="yrfG"
                     /locus_tag="OO357_000361"
     CDS             57489..58157
                     /gene="yrfG"
                     /locus_tag="OO357_000361"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP/IMP nucleotidase"
                     /protein_id="HCR5885427.1"
                     /translation="MHINIAWQDVDTVLLDMDGTLLDLAFDNYFWQKLVPETWGAKNG
                     VTPQEAMEYMRQQYHDVQHTLNWYCLDYWSEQLGLDICAMTTEMGPRAVLREDTIPFL
                     EALKASGKQRILLTNAHPHNLAVKLEHTGLDAHLDLLLSTHTFGYPKEDQRLWHAVAE
                     ATGLKAERTLFIDDSEAILDAAAQFGIRYCLGVTNPDSGIAEKQYQRHPSLNDYRRLI
                     PSLM"
     gene            58168..58569
                     /gene="hslR"
                     /locus_tag="OO357_000362"
     CDS             58168..58569
                     /gene="hslR"
                     /locus_tag="OO357_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated heat shock protein Hsp15"
                     /protein_id="HCR5885428.1"
                     /translation="MKEKPAVEVRLDKWLWAARFYKTRALAREMIEGGKVHYNGQRSK
                     PSKIVELNATLTLRQGNDERTVIVKAITEQRRPASEAALLYEETAESVEKREKMAMAR
                     KLNALTMPHPDRRPDKKERRDLLRFKHGDSE"
     gene            58594..59463
                     /gene="hslO"
                     /locus_tag="OO357_000363"
     CDS             58594..59463
                     /gene="hslO"
                     /locus_tag="OO357_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709173.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HCR5885429.1"
                     /translation="MPQHDQLHRYLFENFAVRGELVTVSETLQQILENHDYPQPVKNV
                     LAELLVATSLLTATLKFDGDITVQLQGDGPMNLAVINGNNNQQMRGVARVQGEIPENA
                     DLKTLVGNGYVVITITPSEGERYQGVVGLEGDTLAACLEDYFMRSEQLPTRLFIRTGD
                     VDGKPAAGGMLLQVMPAQNTQQDDFDHLATLTETIKTEELLTLPANEVLWRLYHEEEV
                     TVYDPQDVEFKCTCSRERCADALKTLPDEEVDSILAEDGEIDMHCDYCGNHYLFNAMD
                     IAEIRNNASPADP"
     gene            complement(59607..61358)
                     /locus_tag="OO357_000364"
     CDS             complement(59607..61358)
                     /locus_tag="OO357_000364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4153 domain-containing protein"
                     /protein_id="HCR5885430.1"
                     /translation="MDNVELSPATRWGMIATGLLQGMIATGLLQGLVCYLLIAWLAGK
                     NHSWIVYGVPATVAFSSVLLFSVISFKQKRLWGWLALVFIATTGMSGWLKWQTDGMNP
                     WRAEKAIWDFGCYLLLMAMLLLPWIQQSLRIRNGSSRYSYFYQSVWHNVLILLVIFLS
                     NGLTWLVLLLWGELFKLVGITFFNTLFFATDWFMYLTFGLVTALAVILARTQSRLIDS
                     IQKLFTLIATGLLPLVSLLTLMFIITLPFTGLSAISRHISAAGLLLTLAFLQLILMAI
                     VRDPQKASLPWTGPLRCLIKTALLVAPLYVFVATWGLWLRVAQYGWTVDRLQGALAEL
                     VLLVWSLGYFVSIVWRNGQNPLVLQGKVNLAVSLLVLVILVLLNSPVLDSMRISVNSH
                     MARYQSGKNTPDQVTIYMLEQSGRYGRAALESLKSDAGFMKDPKRARDLLMALDGEQH
                     LQEQVSEKVFAENVLIAPGSVKPDATFWSALIQDRYNVMTCIEKDACVLVEQDLNSDG
                     QAERILFAFNDDRVIVYGFDSDRKEWDALDMSLLPNEITKEKLLTAAKDGKLGTRPKA
                     WRDLTVDGETLEINLSK"
     gene            61737..63359
                     /gene="pckA"
                     /locus_tag="OO357_000365"
     CDS             61737..63359
                     /gene="pckA"
                     /locus_tag="OO357_000365"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HCR5885431.1"
                     /translation="MRVNNGLPPQELEAYGISDVHDIVYNPSYDLLYQEELDPSLTGY
                     ERGVLTNLGAVAVDTGIFTGRSPKDKYIVRDDTTRDTFWWADKGKGKNDNKPLSPETW
                     QHLKGLVTRQLSGKRLFVVDAFCGANPDTRLSVRFITEVAWQAHFVKNMFIRPSDEEL
                     AGFKPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSM
                     MNYLLPLKGIASMHCSANVGEKGDVAVFFGLSGTGKTTLSTDPKRRLIGDDEHGWDDD
                     GVFNFEGGCYAKTIKLSKEAEPEIYNAIRRDALLENVTVREDGTIDFDDGSKTENTRV
                     SYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTADQTQYHFLSGFTAKLA
                     GTERGITEPTPTFSACFGAAFLSLHPTQYAEVLVKRMQAAGAQAYLVNTGWNGTGKRI
                     SIKDTRAIIDAILNGSLDNAETFTLPMFNLAIPTELPGVDTKILDPRNTYASPEQWQE
                     KAETLAKLFIDNFDKYTDTPAGAALVAAGPKL"
     gene            complement(63435..64787)
                     /gene="envZ"
                     /locus_tag="OO357_000366"
     CDS             complement(63435..64787)
                     /gene="envZ"
                     /locus_tag="OO357_000366"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     EnvZ"
                     /protein_id="HCR5885432.1"
                     /translation="MRRLRFSPRSSFARTLLLIVTLLFASLVTTYLVVLNFAILPSLQ
                     QFNKVLAYEVRMLMTDKLQLEDGTQLVVPPAFRREIYRELGISLYSNEAAEEAGLRWA
                     QHYEFLSHQMAQQLGGPTEVRVEVNKSSPVVWLKTWLSPNIWVRVPLTEIHQGDFSPL
                     FRYTLAIMLLAIGGAWLFIRIQNRPLVDLEHAALQVGKGIIPPPLREYGASEVRSVTR
                     AFNHMAAGVKQLADDRTLLMAGVSHDLRTPLTRIRLATEMMSEQDGYLAESINKDIEE
                     CNAIIEQFIDYLRTGQEMPMEMADLNAVLGEVIAAESGYEREIETALYPGSIEVKMHP
                     LSIKRAVANMVVNAARYGNGWIKVSSGTEPNRAWFQVEDDGPGIAPEQRKHLFQPFVR
                     GDSARTISGTGLGLAIVQRIVDNHNGMLELGTSERGGLSIRAWLPVPVTRAQGTTKEG
                     "
     gene            complement(64784..65503)
                     /gene="ompR"
                     /locus_tag="OO357_000367"
     CDS             complement(64784..65503)
                     /gene="ompR"
                     /locus_tag="OO357_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014646135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator OmpR"
                     /protein_id="HCR5885433.1"
                     /translation="MQENYKILVVDDDMRLRALLERYLTEQGFQVRSVANAEQMDRLL
                     TRESFHLMVLDLMLPGEDGLSICRRLRSQSNPMPIIMVTAKGEEVDRIVGLEIGADDY
                     IPKPFNPRELLARIRAVLRRQANELPGAPSQEEAVIAFGKFKLNLGTREMFREDEPMP
                     LTSGEFAVLKALVSHPREPLSRDKLMNLARGREYSAMERSIDVQISRLRRMVEEDPAH
                     PRYIQTVWGLGYVFVPDGSKA"
     gene            65731..66207
                     /gene="greB"
                     /locus_tag="OO357_000368"
     CDS             65731..66207
                     /gene="greB"
                     /locus_tag="OO357_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreB"
                     /protein_id="HCR5885434.1"
                     /translation="MKTPLVTREGYEKLKQELNYLWREERPEVTKKVTWAASLGDRSE
                     NADYQYNKKRLREIDRRVRYLTKCLENLKIVDYSPQQEGKVFFGAWVEIENDDGVTHR
                     FRIVGYDEIFGRKDYISIDSPMARALLKKEVGDLAVVNTPAGEANWYVNAIEYVKP"
     gene            66304..68625
                     /locus_tag="OO357_000369"
     CDS             66304..68625
                     /locus_tag="OO357_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HCR5885435.1"
                     /translation="MMNDSFCRIIAGEIQARPEQVDAAVRLLDEGNTVPFIARYRKEI
                     TGGLDDTQLRNLETRLSYLRELEERRQAILKSISEQGKLTDDLAKAINATLSKTELED
                     LYLPYKPKRRTRGQIAIEAGLEPLADLLWSDPSHTPEVAAAQYVDADKGVADTKAALD
                     GARYILMERFAEDATLLAKVRDYLWKNAHLVSTVVSGKEEEGAKFRDYFDHHEPLSTV
                     PSHRALAMFRGRNEGVLQLSLNADPQFDEPPKESYCEQIIMDHLGLRLNNAPADSWRK
                     SVVSWTWRIKVLMHLETELMGTVRERAEDEAINVFARNLHDLLMAAPAGLRATMGLDP
                     GLRTGVKVAVVDATGKLVATDTIYPHTGQAAKAAMTVAALCEKHNVELVAIGNGTASR
                     ETERFYLDVQKQFPKVTAQKVIVSEAGASVYSASELAAQEFPDLDVSLRGAVSIARRL
                     QDPLAELVKIDPKSIGVGQYQHDVSQTQLARKLDAVVEDCVNAVGVDLNTASVPLLTR
                     VAGLTRMMAQNIVAWRDENGQFQNRQQLLKVSRLGPKAFEQCAGFLRINHGDNPLDAS
                     TVHPEAYPVVERILAATQQALKDLMGNSSELRNLKASDFTDEKFGVPTVTDIIKELEK
                     PGRDPRPEFKTAQFADGVETMNDLQPGMILEGAVTNVTNFGAFVDIGVHQDGLVHISS
                     LSNKFVEDPHTVVKAGDIVKVKVLEVDLQRKRIALTMRLDEQPGETNSRRGGGNERPQ
                     NNRPAAKPRGREAQPAGNSAMMDALAAAMGKKR"
     gene            69063..69290
                     /gene="feoA"
                     /locus_tag="OO357_000370"
     CDS             69063..69290
                     /gene="feoA"
                     /locus_tag="OO357_000370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter A"
                     /protein_id="HCR5885436.1"
                     /translation="MQYTPDTAWKITGFSREISPAYRQKLLSLGMLPGSSFDVVRVAP
                     LGDPIHIETRRVSLVLRKKDLALLEVEAVSC"
     gene            69307..71628
                     /gene="feoB"
                     /locus_tag="OO357_000371"
     CDS             69307..71628
                     /gene="feoB"
                     /locus_tag="OO357_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(2+) transporter permease subunit FeoB"
                     /protein_id="HCR5885437.1"
                     /translation="MKKLTIGLIGNPNSGKTTLFNQLTGARQRVGNWAGVTVERKEGQ
                     FSTTDHQVTLVDLPGTYSLTTISSQTSLDEQIACHYILSGDADLLINVVDASNLERNL
                     YLTLQLLELGIPCIVALNMLDIAEKQNIRIEIDALSARLGCPVIPLVSTRGRGIEALK
                     LAIDRYKANENVELVHYAQPLLNEADSLAKVMPSDIPLKQRRWLGLQMLEGDIYSRAY
                     AGEASQHLDATLARLRNEMDDPALHIADARYQCIAAICDVVSNTLTAEPSRFTTAVDK
                     IVLNRFLGLPIFLFVMYLMFLLAINIGGALQPLFNVGSVALFVHGIQWIGYTLHFPDW
                     LTIFLAQGLGGGINTVLPLVPQIGMMYLFLSFLEDSGYMARAAFVMDRLMQALGLPGK
                     SFVPLIVGFGCNVPSVMGARTLDAPRERLMTIMMAPFMSCGARLAIFAVFAAAFFGQN
                     GALAVFSLYMLGIVMAVLTGLMLKYTIMRGEATPFVMELPVYHVPHVKSLIIQTWQRL
                     KGFVLRAGKVIIIVSIFLSAFNSFSLSGKIVDNINDSALASVSRVITPVFKPIGVHED
                     NWQATVGLFTGAMAKEVVVGTLNTLYTAENIQDEEFNPAEFNLGEELFSAVDETWQSL
                     KDTFSLSVLMNPIEASKGNGEMGTGAMGVMDQKFGSAAAAYSYLIFVLLYVPCISVMG
                     AIARESSRGWMGFSILWGLNIAYSLATLFYQVASYSQHPTYSLVCILAVILFNIVAIG
                     LLRRARSRVDIELLATRKSVSSCCAASTTGDCH"
     gene            71628..71864
                     /gene="feoC"
                     /locus_tag="OO357_000372"
     CDS             71628..71864
                     /gene="feoC"
                     /locus_tag="OO357_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[Fe-S]-dependent transcriptional repressor FeoC"
                     /protein_id="HCR5885438.1"
                     /translation="MASLIQVRDLLALRGRMEATQISQTLNTPQPMINAMLQQLESMG
                     KAVRIQEEPDGCLSGSCKSCPEGKACLREWWALR"
     gene            72067..>72934
                     /gene="rpnA"
                     /locus_tag="OO357_000373"
     CDS             72067..>72934
                     /gene="rpnA"
                     /locus_tag="OO357_000373"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnA"
                     /protein_id="HCR5885439.1"
                     /translation="MSKKQSSTPHDALFKLFLRQPETARDFLAFHLPAPIHALCDMKT
                     LKLESRSFIDDDLRESYSDVLWSVKTEQGPGYIYCLIEHQSTSNKLIAFRMMRYAIAA
                     MQNHLDAGYKTLPMVVPLLFYHGIESPYPYSLCWLDCFADPKLARQLYASAFPLIDVT
                     VMPDDEIMQHRRMALLELIQKHIRQRDLMGLVEQMACLLSSGYANDRQIKGLFNYILQ
                     TGDAVRFNDFIDGVAERSPKHKESLMTIAERLRQEGEQSKALHIAKIMLESGVPLADI
                     MRFTGLSEEELAA"
BASE COUNT        17577 a        19763 c        18447 g        17147 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcacgagc agcaccgccg taggttttag ccagtacggt ggtgattgca
      121 gcggtcagag tagttttacc gtggtcaacg tggccgatag taccaacgtt aacgtgcggt
      181 tttgtacgtt caaatttttc tttagacacg gctatattcc ttactatagt gctctcccct
      241 tcaggagaga gcacgggact ttggtattaa cccttaggct tatttaccac gggcttcaat
      301 tacggcctga gcaacgttac tcggcgcttc atcatacttc aggaattcca tagtgtatga
      361 tgcacgacct ttggtcagag aacgcagctg agttgcgtat ccgaacattt cagacagcgg
      421 tacttcagcg tggatcttaa cgccagtaac ttcagattcc tgacctttga gcataccacg
      481 acgacggctc aagtcaccga taacgtcacc ggtgttctct tccggagttt ctacttcaac
      541 cttcatgatc ggctcaagca gaactggttt cgctttctta aagccttctt taaaggcgat
      601 agaagcagcc agtttaaacg ccagttcaga ggagtcaacg tcatggtaag aaccgaagtg
      661 cagacgaata cccatgtcta ctaccgggta gcctgccagc ggacctgctt tcagctgttc
      721 ctggatacct ttatcaacgg ccgggatgta ttcgccaggg attacaccac ctttaatgtc
      781 gttgatgaac tcgtagcctt tcgggtttga acccggctcc agcgggtaca tgtcgataac
      841 aacatgacca tactgaccac gaccaccaga ctgtttcgcg tgtttacctt caacatcggt
      901 aactttctgg cggatagttt cacggtaagc aacctgcggt ttacctacgt tcgcttcaac
      961 gttgaattca cgcttcatac ggtcaacgat gatgtcgagg tgcagttcgc ccatacccgc
     1021 gatgatggtc tggttagatt cttcgtcagt ccatacacgg aaagacgggt cttctttagc
     1081 cagacggccc agagccagac ccattttttc ctggtcagct ttggttttcg gttcaactgc
     1141 gatggagatt accggctcag ggaattccat acgttccaga atgatcggcg catccgggtc
     1201 acacagggtg tcaccagtgg ttacgtcttt caggccgata gcagcagcga tgtcgcccgc
     1261 gcgaacttct ttgatctctt cacgtttgtt agcgtgcatc tgaacgatac gaccgaaacg
     1321 ctcacgtgca gctttcacgg agttcagtac ggtatcacca gagttaacca caccggagta
     1381 aacacggaag aaggtcaggt taccaacaaa cgggtcggta gcgattttga acgccagtgc
     1441 agagaacggc tcgtcatcac ttgcgtgacg ttcagccgga gtgtctttac cgtcgtccag
     1501 gataccgttg atcgcaggta cgtcaaccgg ggatggcagg taatcaatta ccgcatccag
     1561 catcgcctga acacctttgt tcttgaacgc agaaccacag gttaccagga tgatttcgtt
     1621 gttcagaacg cgctgacgca gagcaccttt gatttctgct tcagtcagtt cttcaccacc
     1681 caggtatttt tccatcagct cttcagaagc ttcagctgcg gattcgatca ggttctggtg
     1741 ccattcgtta gccagttcaa ccatgtctgc cgggatatct tcgtattcga aggttacgcc
     1801 ctggtcagcg tcattccagt tgatagcttt cattttcacc aggtcaacaa caccggtgaa
     1861 atgttcttca gcaccaatcg ccagctgcag cggaaccggg ttcgcgccca gacgggtttt
     1921 gatctggtta acaactttca ggaagttcgc acccacgcgg tccattttgt taacgaacgc
     1981 aatgcgcgga actttatatt tgtttgcctg acgccatacg gtttcagact gcggctgaac
     2041 accaccaact gcgcagtaaa ccattaccgc accatcgaga acacgcatgg aacgttctac
     2101 ttcgattgtg aagtcaacgt gccccggggt gtcgatgatg ttgatgcgat gcggctcata
     2161 ctgcttagcc ataccagacc agaatgcagt agtcgcagcg gaagtgatgg taataccacg
     2221 ttcctgctcc tgctccatcc agtccatggt tgcagcgccg tcatgaactt caccgatttt
     2281 atggtttaca ccggtgtaga acagaatacg ttcggtagta gtggttttac cggcgtcgat
     2341 gtgcgcactg ataccgatgt tacggtagcg tgcgatgggt gttgtacgag ccatttgttt
     2401 cctcgtttat cttttaggcg ttcaatttaa gtagcccaaa gcgggctgct tactggaagc
     2461 gcccgcctgg tgactaaaac tccgaaggga ttaccaacgg tagtgtgcga acgccttgtt
     2521 ggcttcggcc atacggtgaa cgtcttcacg tttcttaact gcagtacctt tgttttctgc
     2581 agcatcagaa agttcgttcg ccaggcgcag agccatggat ttatcaccgc gtttacgagc
     2641 agcttcaacg atccaacgca ttgccagagc attacgacga accggacgga cttcaactgg
     2701 tacctgataa gtagaaccac caacgcggcg agacttaact tctacagtcg ggcgcacgtt
     2761 ttcgagagct acttcgaatg cttccagttc agatttacca gagcgctgag ccagggtctc
     2821 cagcgcgctg tatacgatag attcagcagt agatttttta ccatctacca tcaggatatt
     2881 tacaaattta gccagcagtt ctgatccgaa cttcggatcc ggcagaattt tacgctgacc
     2941 aatgacgcga cgacgtggca tggaaatact ccgttgttaa ttcaggattg tccaaaactc
     3001 tacgagttta gtttgacatt taagttaaaa cgtttggcct tacttaacgg agaaccatta
     3061 agccttagga cgcttcacgc catacttgga acgagcctgc ttacggtctt taacgccgga
     3121 gcagtcaaga gcaccacgta cggtgtggta acgaacaccc gggaggtctt taacacgacc
     3181 gccacggatc aggatcacgg agtgctcctg caggttgtga ccttcaccac cgatgtagga
     3241 agtcacttcg aaaccgttag tcagacgaac acggcatact ttacgcagcg cggagttcgg
     3301 ttttttagga gtggtagtat atacacgagt acatacgcca cgtttttgcg ggcatgcttc
     3361 cagcgcaggc acgttgcttt tcgcaacttt gcgagcacgt ggtttgcgta ccagctggtt
     3421 aactgttgcc attaaatagc tcctggtttt agcttttgct tcgtaaacac gtaataaaac
     3481 gtcctcacac aatatgagga cgccgaattt tagggcgatg ccgaaaaggt gtcaagaaat
     3541 atacaacgat cccgccatca ccaggccatc tggctggagt gcttaaccgt aagtctgacg
     3601 aaatcagtat agtcaatggg aatgatgtcg ttcaaaattt gaccagtcaa accgcgggca
     3661 ataaggtctt cgttcagggc atagacctta atgggggcat tacgcagact ttcaaggtag
     3721 cggttaccgt caactgcggc agttacgcca tcttgcaata atagcagttc gtctccttca
     3781 ctgagcagac gcagcagcgc agcaaaatcc gtcagccagg gtgaacgatg taacgtgtgc
     3841 agcataaacg cctcaaaacc tcaaaataac atcgtagttg gcgagttcgc ggcgtaaggc
     3901 atctgcttcg agcggcgtgg cttcgacaac aaagggtgtc tgcggatcta acccgcgttc
     3961 gcgcagtgaa gccgcacaaa cccagcactg ttcaatgtcg tacagactca acaatttaaa
     4021 agtggcaatg taatcacgcg ccagcactgc atcgggcttt tgtcctgaca gcagctgaaa
     4081 aacgccatca gcaataaaga agacggccag atcgtcagtt aatgcggaag ttgccagtaa
     4141 agcatctaaa ccttcccgac cagcagctgt accatgaggt gcagtagaaa aaacaaacgc
     4201 aattcgtttc atcagaactg taccaccctg tcacaggtca gcgaggcttc cgccagcgcg
     4261 ccaagtccgc ttaaggtaaa tcccggctga aggtttgacg aagccagccc cagccttcct
     4321 gcttcagttt catcgacgat accgcggcgt aacgctgccg ctacgcagat attcagcgcc
     4381 acaccatgtt gcgcattcaa ctgttgccag ccgcgcacaa ggtcaaattc gtcgcttgcc
     4441 ggtgaggtca gttggttagc gttatagacc ccttcccgat agaaaaagac gctgcttaac
     4501 ttatggcctt ctgctatcag cgcctgcgca aactgaaaag cactactcgc ctgttgcgta
     4561 ccgtatgctg gcccggtcac cacgatggca aaacgcatta cttatcttgc ccctgaaaat
     4621 caccgctctt gaactggcgg atgtagagat agacagtgtg tttggagatg ttcaggcggt
     4681 cagcaacctg gttgatcgca tctttaatat cgaagatccc tttctcgtag agattcagca
     4741 cgatctgacg atttttggcg ttattagaaa cattgcgatc ggcattcact tcttcgatgg
     4801 tgaactccag cgtttgggta accagatctt caacagaaga ggcaaagttg acgcttgaac
     4861 cgacatccgg ggtttctggc ggcacaaaag tgctcataat ctgcgagaag ggaacatcaa
     4921 gattcatatt gatgcacagc agaccaatta cacgctgttc gcggttacga atcgcgatag
     4981 tcagggactt cattaatacg ccgcttttgg cgcgagtaaa gtagcattta gaaacgctgc
     5041 tatccgctcc cgtcatatcg tgcagcatac gtagcgccag gtcagtaatt ggcgaaccaa
     5101 tcttccggcc tgtatgttca ccgttagcaa tgcgaatggc tgaacatttt agatcctgca
     5161 aagagtgcaa aacgatttca cagtgggagc caataagcat cgctaacccg tccaccaccg
     5221 cttcgtagga tttcagaata tcaaaatcgg tctggtcgaa aggacgttga tccagtaaat
     5281 ccaactcact ggtttcgttg gttaaaagcg acctggacat gaaaaaaaac actccttttc
     5341 aggagcctgt cgttaacttt tcagggcagg ctcattaatg atgcgggtaa ctaaattaat
     5401 acagcggagg ttccgctttc cagcactaat tatatccggc ctgtaataaa aaaaccgccg
     5461 cctggtcagg cggcggttct taatgcttat tttttagcag aatctgcggc tttcgcatca
     5521 gcttccggct ttgcatcagc cttcggcgct ggtttcacat ccagcagctc tacgtcaaac
     5581 actagggtag aattcggtgg gatccccgga acacccgctt tgccgtaagc cagttctggt
     5641 ggaataacca gtttgatctt accgcctttc ttgatgttct tcagaccttc tgtccaaccc
     5701 gggataacac cgtccagacg gaaagaaagc ggttcaccac gggtgtaaga gttgtcgaac
     5761 tctttaccgt cgatcagcgt acctttgtag ttcactacaa cagtatcgct gtctttcggt
     5821 gcttcgcctt taccggcttc tactacctga taaaccagac cagttgaaga ggttttcaca
     5881 cctttctctt tggcaaattt ctcgcggtac tctttacctt ttgcttcgtt atcagccgcg
     5941 tctttttcca tcttcgcctg agcagaagac ttcacgcgag cttcgaatgc ttgcagagtc
     6001 tgttcgatct cttggtcgga gagtttgctc ttatcagcaa atgcatcctg aacaccagcg
     6061 atcagctgat ctttatccag tttgatgccc agtttttctt gttctttaag agagttttcc
     6121 atgtaacgac ccagcgaagc acccagtgca taagctgatt tctgatcgtc atttttgaac
     6181 gatgctttgc tgtctgcagt tgtagcaggt tttgcagctt cagcagcaaa agtgattggt
     6241 gcatgcaggg caacggccat tgtggtcgcc agtagcgtta ctttaaacag tgatttcatc
     6301 catatctcca ggatcggggc atctcacccc agggttaact attatcagaa gggtactata
     6361 aagcgttgtc gaacaaatct acatacagac acgccctatt atcatctatt ttcagactct
     6421 ttttgtttaa attagtttcg atgaccgcga aatgagtgct gtctcgggca gcaaagttaa
     6481 gtagaatccg cggcgaccat tcgacaaaag aggtgaatca tgcaggattt atcattggaa
     6541 gcacgcctgg cagagctgga gagccgactg gcttttcagg aaatcaccat tgaagaactg
     6601 aacgtcacgg tggccgctca tgaaatggag atggcgaaac tgcgcgatca tctgcgtctg
     6661 ctgaccgaga agttaaaagc cagccagccg tcgaacatcg cgtcgcaggc tgaagaaacg
     6721 ccaccgccac attattgagg cgtaaaaaaa gcggggattc cccgcttttt tgtcaccttt
     6781 tcggtattag tggcaaccac aaccgccgtt gcctttaccg ccacagcagc cttcgccacc
     6841 gtgttcatga ccgtgatcgt ggccatgacc gccgcagcaa ccgtcgtggt cgtgatcgtg
     6901 gtgatgatcg tgcgcgccgt gaacgtggcc atgagccagt tcttcttcgg tcgcttcgcg
     6961 aatcgccaca acttcaacgt tgaatttcag gttctgaccg gccagcatgt ggttaccatc
     7021 aaccactacg tgatcgtctt caaccgcagt gatttcaacc ggtaccggac cctggtcagt
     7081 ttcagccagg aaacgcatac ctacctgcag ttcatcaacg cccataaata cgtctttagg
     7141 aacacgttgc actaggtttt cgtcgtactg accgtaagcg tcgttcgcgc caacagcgac
     7201 atcaaatttg tcgccaactt catgaccttc cagcgccgtt tccaggccag agatcaggga
     7261 accgtgacca tgcaggtagt ccagcggcgc actcaccgga gactcatcaa ccaacacacc
     7321 gtcttctgta cgtacctgat aggccaggct gaccaccagg tcttttgcta ctttcatgat
     7381 atctcctgag catgggaaga atagtggcgc agattgtagc ggaattctgc agccgtgtac
     7441 tcactagctt aaaaaaactc ggcgcatatc gctagtccgg atgaaaaatc ccgatcactt
     7501 gctcatcttt gtgaacgtga tcgcgggctt ctttgtctgc ttctcgcatc tgatgtccgc
     7561 acttaacaca ttcaacaata tcaatattat tttcgcgcca catcgccatt gaatcctgcg
     7621 cctgacaggc cgggcatttt gcgcccgcta taaaacgttt tcggattgcc atccttaccc
     7681 tctactcaaa ttcatcccag ccgtccagct ggcgtcgttc ttgttgcatt tcacgctgga
     7741 aaatctcttc cagttcgcgt cgggcttccc tggcgcgaga aatttgtacg gtatcagcat
     7801 gcatcgggat taactcccgc agcattcgca tatccaggcg gcgaaaatgc agttgcgccc
     7861 gctgtgcctg atgcggatgc atgccaagcg tgaccagcgt cttgcgcccc agctctaacg
     7921 cactggagaa tgtttcacgg gaaaactgcg tcacccctgc ctgtaataac tcatgcgctt
     7981 ccacacgtcc gcgcgctcgc gcaagaatat gcaaatgcgg aaagtgctgc tggcatattt
     8041 ccaccagctt catggtgtct tccggctcgt tacaggtaat gacgatagac tcagcggcct
     8101 ctgcacccgc agaacgtaaa agatcgacct gcgtggcgtc gccgtaataa actttgtagc
     8161 catatttacg catcaggtta acggcgctga tatcccgctc cagcacggta atgcgcattt
     8221 tatttgccat cagcaaacga ccaatcacct gaccaaaacg cccgaagccc acgacaatga
     8281 cctggggttt atcatcgttg acccacggtt tttcgtcttc ttcttccggt ccgttaaact
     8341 ggcgggatag ccatttatcc accagcttca tcagcaacgg cgccatctgg tcgccctgga
     8401 ataaacgttg tgaagaagcg gtagaaaaga ggacaaaggc aaactcaccg ccctgactca
     8461 acacgccagc aaactgcatc cgctctgagc tacgcacgcc atacaatcgc gccagcagat
     8521 acagcacgag aattttcacc gccaccagca caaccacgct tatcactacc cacaacagat
     8581 gggtataaag caccccgagg ttgagcgaca tgccgacaga gataaagaac aaaccgagca
     8641 gcaagccttt gaagggatcg atagccgttt ccagttcatg gcgatattca ctttccgcca
     8701 gcagcacgcc cgcaataaat gtaccgagcg ccatcgacag ccccagcgca tccataaaca
     8761 atgcggaacc caacaccagc agcagcgtcg cggcggtgaa cacttcccgc acgccagaag
     8821 ctgcaataaa gcggaatacc ggacgcagta aatagcgccc accaatcagc atgccgacaa
     8881 actccagcac cttcatgccg atcttcatcc agtcgaaatg ttcgtctgcc gaccccgcca
     8941 acaacggcac taacgccagt gctgggatca ctgccagatc ctgaaacagc agaaccgaaa
     9001 acccgagctg gccggattcg ctgcgattca tccctttctc acgcatcaat tgcaacgcca
     9061 ttgcagttga agacatcgca aagccaatgc caccgaccac cgccgcctgc caggcgaaat
     9121 ccgtcagcat caataatccc gccagcaacg ccgcgcttaa cagcacctgt gccgcgccta
     9181 cgccaaaaat cgaacgccgc agttgccaaa gtttggaggg attcaactca aggccgatga
     9241 taaacatcag gaataccacg ccgagttccg aaaagtggag gatctcatcg acgtcgctaa
     9301 taaaccccag cccccacggg ccaattgcaa tccccgccag caaatatccc aacacagcgc
     9361 caatccccag ccgcgaagag aaacagcact cctgcgagta aaaaatcgga accttccatc
     9421 agcggcctcc tggagacagc ggatttgcca accagtcacc gtaggctctg gcgtggctcg
     9481 ccagctcctt tgcgctttgc cgtctcgccc agtaaataat gatgggactt aaccaatgca
     9541 tccggcacat gcccgccgcc agttcaaagg ggcgcagcac atcgctcatc gggtagcgat
     9601 tcagcgcgtc ataacggtaa gcactttccg gctcgccggt ggtaatcacg ttacgccagt
     9661 actttcccgc cagttggttt cctcccggcc cgctggcaaa accacgactt aatacccggt
     9721 ccagccactc tttcagtagc gccgggcagc tataggtata aagaggatgc tgaaagacaa
     9781 tcacctcgtg ctcgcgcagt aatgcctgct cacgggggat atcaataaaa aaatcgggat
     9841 agtgcgcgta aaggtcgtgc acggtaacat tgctgagctg cgtggccggt ttaagcagta
     9901 cccggtttgc caccgagtcc tgagattccg gatgggcata cagcagcaaa actttcgctg
     9961 gctgagacat catccccctc ccggtatggt ttttgtgtat agtcgctgtt ttgggctacc
    10021 attgcgcccg gtgcggcagc tcgcccatac attacattat cataatgata agttaacata
    10081 gtctgaacat acggcgcctt atgattgttt tctcctcgtt acaaattcgt cgcggcgtgc
    10141 gcgtcctgct ggataatgcc accgccacca tcaaccccgg gcagaaagtc ggcctggtgg
    10201 gtaaaaacgg ctgtggtaaa tctaccctgc tggcattgct gaaaaatgaa atcagcgccg
    10261 acggcggcag ctacaccttt ccgggaagct ggcaactggc gtgggtgaat caggaaacgc
    10321 cagcgttacc gcaagcggcg ctggaatatg tcattgacgg cgaccgtgaa tatcgtcaac
    10381 tggaagcgca gctgcacgac gccaacgaac gtaacgacgg gcacgccatt gcgaccattc
    10441 atagcaagct ggatgctatt gacgcatgga gtattcgctc ccgtgccgcc agcctgctgc
    10501 acggcctcgg tttcagcaat gaacaactgg agcgcccggt aagtgatttc tccggtggct
    10561 ggcgtatgcg tcttaacctt gcccaggcgc tgatttgccg ttcagacttg ctgctgctcg
    10621 acgaaccgac taaccacctc gatctcgatg ccgttatctg gctggaaaaa tggttgaaga
    10681 gctatcaggg cacgctgatc ctgatctctc acgaccgcga cttcctcgat ccgatcgttg
    10741 ataaaattat tcatatcgaa caacaaagca tgttcgagta caccggcaac tacagttcgt
    10801 ttgaagtaca gcgcgccacc cgtctggcgc agcaacaagc gatgtacgaa agccagcagg
    10861 aacgcgtggc acatctgcaa agttatatcg atcgtttccg tgccaaagcc accaaagcga
    10921 agcaggccca gagccgcatt aagatgctgg aacgtatgga gctgattgcc cccgcacacg
    10981 tcgacaaccc gttccgcttt agcttccgcg cgccggaaag cctgccaaat ccgttactga
    11041 agatggaaaa ggtcagcgca ggctatggcg atcgcattat tctcgactcg attaaattga
    11101 atctggtccc cggctcgcgc attggtctgt taggccgcaa cggcgcaggt aaatcgacat
    11161 taatcaaact gttagccggt gaacttgcgc cagtcagcgg tgaaattggt ctggcgaaag
    11221 ggatcaagct cggctacttc gcccagcatc aacttgaata cctgcgcgcc gacgaatcac
    11281 ctattcaaca tctggcacgt ttagcgccgc aggagctgga acaaaaactg cgtgactacc
    11341 tcggcggctt tggtttcctg ggcgataaag taaccgaaga aacgcgccgc ttctccggtg
    11401 gggaaaaagc ccgcctggtg ctggcattaa ttgtctggca gcgtccgaat ctgctgctgc
    11461 tcgacgaacc gactaaccac cttgacctcg acatgcgtca ggcactcacc gaagcattaa
    11521 tcgagtttga aggcgcgctg gttgtcgttt cgcacgaccg tcatttgctg cgttccacca
    11581 ctgacgatct ctacctggtt cacgatcgta aagtcgaacc gttcgacggc gatctggaag
    11641 attatcaaca gtggttgagc gacgtacaaa agcaggaaaa ccagaccgac gaagcgccaa
    11701 aagagaacgc gaacagcgcc caggcacgta aagatcagaa gcgtcgggaa gcggagctgc
    11761 gtgcgcaaac ccagccgctg cgtaaagaga ttgcccgtct ggaaaaagag atggagaagc
    11821 tgaacgcgca actggcgcag gcggaagaga aactcggcga cagcgaactg tatgaccaga
    11881 gccgtaaagc ggagttgacc gcctgcctgc aacagcaagc cagcgccaaa tccggcctgg
    11941 aagagtgcga aatggcgtgg ctggaagccc aggagcagct tgagcagatg ctgctggaag
    12001 gccaaagcaa ctgatggcgc agataacgac gaccgatgcc aatgaattta gcagcagtgc
    12061 tgaattcacc cctatgcgcg gctttagcaa ttgtcatcta caaactatgc tgccgcgttt
    12121 gtttcgtcgt caggtgaaat tcaccccgta ctggcagcgg ctggagttgc ccgacggcga
    12181 ttttgtcgat ctcgcgtgga gtgaagaccc cgcacaggcg aaacataaac cgcgtttagt
    12241 ggtgtttcac gggctggaag gcagtctcaa tagcccttac gcccacggtc tggttgaggc
    12301 ggcgcaaaag cgcggctggc tgggcgtggt gatgcatttt cgcggatgca gcggtgaacc
    12361 aaaccgtatg caccgcattt accattcggg cgaaaccgaa gacgccagtt ggtttttacg
    12421 ctggctgcaa cgcgaatttg gtcatgcgcc aacggctgcc gtcggctatt cgctcggcgg
    12481 taatatgctg gcctgtttgc tggcaaagga aggcaatgat ctcccggttg atgcggcggt
    12541 gattgtctct gcgccgttta tgctggaagc ctgtagctat catatggaaa agggcttttc
    12601 ccgcgtttat cagcgttact tgctgaacct gttaaaagcc aatgccgcgc gcaagctggc
    12661 agcctacccc ggaacgctgc cgattaatct cgcgcagtta aaatcggtac gtcgcatccg
    12721 tgaatttgac gatctaatca ccgccagaat tcacggctac gccgacgcta tcgactatta
    12781 tcgtcagtgt agcgccatgc caatgctgaa ccggatcgcc aaaccgacgc tgattattca
    12841 cgccaaagac gatccgttta tggatcatca ggtgatccca aaaccggaaa gtctcccctc
    12901 gcaggtggag tatcaactga ctgaacatgg cggtcatgtt ggctttattg gcggtacatt
    12961 acttcatccg caaatgtggc tggagtcacg cattcctgac tggttaacaa cgtatctgga
    13021 ggcgaaatca tgttgattcc gtggcaagac ctctcccccg aaacgctgga aaatttaatt
    13081 gaaagctttg tgttacgtga aggcaccgat tatggtgaac atgagcgtac acttgaacag
    13141 aaagtcgccg acgtcaaacg ccagctacag tgcggagaag cggtgctggt atggtcggag
    13201 ctgcacgaaa cggtcaatat tatgccgcgc agtcagtttc gcgaataacc tgcaaccgtc
    13261 ggcggacgag ataatagtga tagctaagtc atggagttac tatgtctgcc aaacatccgg
    13321 tcattgcggt aacaggatcc agcggcgcgg ggaccaccac caccagcctc gcgtttcgta
    13381 aaatattcgc gcagttaaat ctgcatgcag ctgaggtgga aggcgacagt tttcaccgtt
    13441 acacccgccc ggaaatggac atggcgatcc gcaaagcgcg cgacgccggg cggcatatca
    13501 gctacttcgg ccccgaagcg aacgacttcg gcctgctgga acaaaccttc attgaatacg
    13561 gtcagagcgg caaagggaaa tctcgcaaat atctgcatac ctacgacgaa gccgtaccct
    13621 ggaatcaggt accgggaaca ttcaccccct ggcaaccttt accagaaccc actgatgtac
    13681 tgttttatga aggtttacac ggcggcgtag tcacgccaca gcataacgtt gcgcagcatg
    13741 tggacttact ggtcggcgta gtgcctatcg ttaaccttga gtggattcaa aaactgatcc
    13801 gcgacaccag cgagcgcggg cactcacgag aagcagtgat ggactcggta gtgcgttcaa
    13861 tggaagacta tatcaactac atcacaccgc agttttcccg cacccatctt aacttccagc
    13921 gcgttcccac cgtcgacact tcaaacccgt tcgcggcaaa aggtatcccg tcgctggatg
    13981 aaagctttgt ggtgatccat tttcgtaatc tggaagggat cgatttccct tggctgctgg
    14041 cgatgttgca aggctcattc atttcccaca tcaatacgtt agtggtacct ggcggcaaaa
    14101 tggggctggc aatggaatta attatgctgc cgctggtgca acgattgatg gaaggaaaga
    14161 aaatcgagta actttgctat tatgccggat aaaatactat ccggcttcac aacgggatag
    14221 ttaagtcacg cggcaaccac ttcatacgag tgagtaatat tcaccgcttt ttccagcatc
    14281 aacgccactg aacaatattt ctcggcagag agatcaaccg cacgcgcaac cgctgcgtct
    14341 ttcaggtcgc gaccggtgac gataaaatgc agattaatgt gcgtaaacag gcgtggtgcc
    14401 tcttcgcggc gttcagaggt caattttact tcacaatcga ccacatcctg acgccctttt
    14461 tgcaggatcg aaaccacatc gatggcactg caaccacccg ccgccatcaa caccatttcc
    14521 atcggacttg gtgctttatc gcctgagttg ccgtccatta aaatctggtg accagaggcg
    14581 gattcgccca gaaaagttaa cccttcgacc cacttcactc gcgcttgcat ttttgctact
    14641 ccactgcgtc aattttcctg acagagtacg cgtactaacc aaatcgcgca acggaaggcg
    14701 acctgggtca tgctgaagcg agacaccagg agacacaaag cgaaagctat gctaaaacag
    14761 tcaggatgct acagtaatac attgatgtac tgcatgtatg caaaggacgt cacattaccg
    14821 tgcagtacag ttgatagccc cttcccaggt agcgggaagc atatttcggc aatccagaga
    14881 cagcggcgtt atctggctct ggagaaagct tataacagag gataaccgcg catggtgctt
    14941 ggcaaaccgc aaacagaccc gactctcgaa tggttcttgt ctcattgcca cattcataag
    15001 tacccatcca agagcacgct tattcaccag ggtgaaaaag cggaaacgct gtactacatc
    15061 gttaaaggct ctgtggcagt gctgatcaaa gacgaagagg gtaaagaaat gatcctctcc
    15121 tatctgaatc agggtgattt tattggcgaa ctgggcctgt ttgaagaggg ccaggaacgt
    15181 agcgcatggg tacgtgcgaa aaccgcctgt gaagtggctg aaatttcgta caaaaaattt
    15241 cgccaattga ttcaggtaaa cccggacatt ctgatgcgtc tgtctgcaca gatggcgcgt
    15301 cgtctgcaag tcacttcaga gaaagtgggc aacctggcgt tcctcgacgt gacgggccgc
    15361 attgcacaga ctctgctgaa cctggcaaaa caaccagatg ctatgactca cccggacggt
    15421 atgcaaatca aaattacccg tcaggaaatc ggtcagattg tcggctgttc tcgtgaaacc
    15481 gtgggacgca ttctgaagat gctggaagat cagaacctga tctccgcaca cggtaaaacc
    15541 atcgtcgttt acggcactcg ttaatcccgt cggagtggcg cgttacctgg tagcgcgcca
    15601 ttttgtttcc cccgatgtgg cgcagactga tttatcaccc cgatatcaac tatgcacttc
    15661 gacaaacgct ggtgctatgt ttgcccgtgg ccgttgggtt aatgcttggc gaattacgat
    15721 tcggtctgct cttctccctc gttcctgcct gttgcaatat tgcgggcctt gatacgcctc
    15781 ataaacgttt tttcaaacac ttaatcattg gtgcgtcgct gtttgccacc tgtagcttac
    15841 tgacacagct gctactggta aaagatgttc ccctgccctt tttgctgacc ggattaacgc
    15901 tggtacttgg cgtcactgct gagctggggc cattacacgc aaaattgctt cctgcatcgc
    15961 tgctcgccgc catttttacc ctcagtctgg cgggatacat gccggtctgg gaaccattgc
    16021 tcatctatgc gttgggcact ctctggtacg gattgtttaa ctggttttgg ttctggatct
    16081 ggcgcgaaca accgctgcgc gagtcactaa gtctgctgta ccgtgaactg gcagattatt
    16141 gtgaagccaa atacagcctg cttacccagc acaccgatcc tgaaaaagcg ctgccaccgc
    16201 tgctggtgcg ccagcaaaaa gcggtcgatt taattactca gtgctatcag caaatgcata
    16261 tgctttccgc gcaaaacaat accgattaca agcggatgct gcgtattttc caggaggcgc
    16321 tggatttgca ggaacatatt tcggtcagtt tgcatcagcc ggaagaggtg caaaaactgg
    16381 tcgagcgtag ccatgcggaa gaagttatcc gctggaatgc gcaaaccgtc gccgctcgcc
    16441 tgcgcgtgct ggctgatgac attctttacc atcgcctgcc aacgcgtttt acgatggaaa
    16501 agcaaattgg cgcactggaa aaaatcgccc gccagcatcc ggataatccg gttgggcaat
    16561 tctgctactg gcatttcagc cgcatcgccc gcgtgctgcg cacccaaaaa ccgctctatg
    16621 cccgtgactt actggccgat aaacagcggc gaatgccgtt gcttccggca ctgaaaagtt
    16681 atctgtcact aaagtctccg gcgctacgca atgccggacg actcagtgtg atgttaagcg
    16741 ttgccagcct gatgggcacc gtgctgcatc tgccgaagtc gtactggatc ctgatgacgg
    16801 tattgctggt gacacaaaat ggctatggcg caacccgtct gaggattgtg aatcgctccg
    16861 tgggaaccgt ggtcgggtta atcattgcgg gcgtagcgct gcactttaaa attcccgaaa
    16921 gttacaccct gacgttgatg ctgattacca ccctcgccag ctacctgata ttgcgcaaaa
    16981 actacggctg ggcaacggtc ggttttacta ttaccgcagt gtataccctg caactgttgt
    17041 ggctgaacgg tgagcagtac atccttccac gtcttatcga taccattatt ggttgtttga
    17101 ttgctttcgg tggtaccgtc tggctgtggc cgcagtggca gagcgggtta ttgcgtaaaa
    17161 acgcccatga tgctttagaa gcctatcagg aagcgattcg cttgattctg agcgaggacc
    17221 cgcaaccaac gccactggcc tggcagagaa tgcgggtaaa tcaggcgcat aacactctgt
    17281 ataactcatt gaatcaggcg atgcaggaac cggcattcaa cagccattat ctggcagata
    17341 tgaaactgtg ggtaacgcac agccagttta ttgttgagca tattaatgcc atgaccacgc
    17401 tggcgcggga acaccgggca ttgccacctg aactggcaca agagtattta cagtcttgtg
    17461 aaatcgccat tcagcgttgt cagcagcgac tggagtatga cgaaccgggt agttctggcg
    17521 atgccaatat catggatgcg ccggagatgc agccgcacga aggcgcggca ggtacgctgg
    17581 agcagcattt acagcgggtt attggccatc tgaacaccat gcacaccatt tcgtcgatgg
    17641 catggcgtca gcgaccgcat cacgggattt ggctgagtcg caagttgcgg gattcgaagg
    17701 cgtaatacag gctaaaccat cggataaggc attcacgccg catccgacat attttgcctg
    17761 atacgtctta tttacgcccc aaccaccttc gccaccgcgt gggcgaaacg ttgcatccct
    17821 tcatcgatat ccgcatcttc caccaccagc gacggtgcta aacgcatcac atccggtccg
    17881 gcattcagca ccattacgcc aacctctgca cccgcataca ggaaatcacg cgcccgacct
    17941 ttgtagtgtg gtttcagctc cgcgccaatt aacagcccca taccgcgaat atcgctaaac
    18001 acatcgtact gctgatcgat cttctgcaaa tggtcaacaa aacgctggcg tttcgcctga
    18061 atgccttcca gcacttcagg ggtattgatg atatcaaacg ccgcgcccgc tactgcacag
    18121 gccagaggat taccgccgta ggtggaaccg tgagaaccag gatgaaacgc agaagcaatt
    18181 tccgccgtgg tcagcatggc gctaatcggg aagccgccgc ctaacgcttt cgcagaagtc
    18241 agaatatccg gcgtcacacc gtagtacctg taagcaaaca aatcgccagt ccgccccatc
    18301 ccgcactgca cttcatcaaa caccaataat gcctgatgtt gatcgcacag ctcgcgcaag
    18361 ccctgcaaaa actctggcgt cgctgccgtc acaccgccct cgccctggat cggctcaacc
    18421 accaccgcac aggtgtgatc atccatcacc gctttcactg catggagatc gttaaaggga
    18481 acgtggatga tgtctgccgg tttcggccca aagccgtcgg aatattttgg ctgcccaccc
    18541 accgaaacgg taaacagcga gcgaccatga aaagcgttat ggaaggcaat aattttggtt
    18601 ttgaacgggc tatgacgcac acaggcgtaa tggcgtgcca gtttaaaggc ggtttcgtta
    18661 gcttccgtgc cggagttcat aaacaccacg cgttcggcaa acgtcgcctc aatcagtttg
    18721 cgcccaagac gcagcgccgg ttcattggtg aaaacgttac tgatatgcca cagagtttcg
    18781 ccctgggttt ttaacgcgtt caccagcgca ggatggcaat ggcccaacgc cgtaactgca
    18841 atgccacccg cgaaatcgac atactccttg ccttgctgat cccagattcg gctgccctga
    18901 ccttttaccg gaataaactc tgccggagca taaatcggca ggatcacttc atcgaaagtc
    18961 gcgcgtgtaa ttgctgtttg ttcaattgcc atctcatgat caccctgtta cgcataaata
    19021 aatgtgaaat tataatcaca aaatatgcat aaaaaatcac tgaatggcaa tcggaaatca
    19081 gagatgcagg aaattagcca gcagttgatg tccttgttcg ctaagaatac tttctggatg
    19141 gaactgcaca ccttccagat cccactggcg atggcgaatc cccataatct cgcgggtttc
    19201 gctccaggcc gtcacgtcaa agcacgctgg taatgagtca ggttccacca ccagcgaatg
    19261 gtagcgtgtc acggtaagtg gatttgccag cccccgaaat acgccctcac cgctatgtgt
    19321 aatcggcgag gttttgccgt gcatgacctt tgcggcgcgc acaactttgc cgccaaatgc
    19381 ctgcgccatt gcctgatgac cgaggcagac gccaagaatc ggcaagcgcc cggcatagtg
    19441 gcgaataacg tcaagggaga tcccggcttc atctggcgta caggggccag gtgagatgac
    19501 aattttttgt ggtttaaggg cgtcgatatc cgccagcgtc aacgcatcgt tgcgcttaac
    19561 cagcacatcc gcccccagtt cacaaaagta ctggtagagg ttccaggtaa aagaatcgta
    19621 gttatctata agcaggatca tggcggctcc ggtacgaaag aaccgcgcta ttttactcag
    19681 attccccggc ttcgcttacc actttgctaa atatcatctg cagtgcggtg agatccccca
    19741 ttgccccact ctgatttgcc tgattccagg cctctttttc gataccctgc cagctcagtt
    19801 gatatccggc atgaatcgcc agttgctcga agaagatccg ctgtgccaga ccacttccca
    19861 cccggaaggg atgcagcacg ttgatttcac aatagtaatg cgccagccgc tcgacgaact
    19921 tcgctttctc caggccaacc agataacctt cttcctccag atcctgcatc agggcattgc
    19981 cctctttttc gatataagca aagtggcaga acggcgtatc gccctgataa atatcaactt
    20041 cacgcagttg ccctgcccag tcgaaaatat cctggtacag ctggcgatgg atagtgcgca
    20101 aatgcggtaa accgcgcacc agcggaccaa gctcaatggt cgcagcacgc agcgccgtca
    20161 tttcgtaagc ggcctgttcc agccgctgct gctggcggat gttcagccgg ttacgcatga
    20221 tatcaaggcc aggataaaga tacagatcgc gcccttcgcc gaatttatcg ctcatagtgc
    20281 ctcctcagct cttcaaggcg cgctaaagcc tctgcggcag taagagtgac taaaggcatc
    20341 tcgacgcctt caagacggcg actggcctgg aaattacgat tacgctgaag ctcccagaga
    20401 cgggactttt gcttatcggt gagtttcttc acttgatgcc tccctgaacg tgtccatttg
    20461 ccacaagtat aagcggcaaa tccgggttac gccgggagag cagaagcgcg ggcaagattg
    20521 cccgcgcgag aaatcattac ggcaggactt tagcggaaag gataactacc ggttttgacg
    20581 gcacattctg gtacggacca acatcatgag tcggcacctg ggaaatctta tcggcaacgt
    20641 ccatgccttt caccacttta ccaaataccg cgtaaccgaa atcacgctga ccatggtcaa
    20701 ggaaggcgtt atcggcaacg ttgataaaga actggctggt ggcgctgtct ttgtcagcgg
    20761 tacgcgccat cgcgatggtg ccacgcgtgt tgcgcaggcc gttatcggct tcatttttga
    20821 ttggcgggtt tggttttttc tgctgcatct gctcggtgaa accgccgccc tgaatcataa
    20881 agccaggaat gacgcggtga aaggtagtgt tgttataaaa accgctgttc acataatcga
    20941 caaagttttg cacagacact ggcgcttttt gtttatccag ctccagttcg atgttaccag
    21001 ctgaggttgt caacaatacg tgcgggtccc ctttcgctgc cattgctgcg ggagaaagag
    21061 cagaaagagc agaaagagcg aaaacagcag ccatcgccgc cagggtcgat ttgaacatga
    21121 gatttcctta acaaacagag cagaaaaaaa gcaagtgaag agattctaaa tagccttcaa
    21181 gaacaaggcc atccctttac ctaattttac gtatctgaaa tatatgtaac ctgacgatca
    21241 ctttttattc cgtgatcaaa atcacctctt aaaatgcaat ttagcaaccg attgcaataa
    21301 aacatttaaa cagatcataa aatcacctaa aatcgcccgc tcgcggcata tcccgctatg
    21361 ccattttatt tttgaacgca ggccaatcat gactaacagc aatcgcatca agctcacatg
    21421 gattagcttt ctctcctacg cactgactgg tgcgttggtt attgtcaccg ggatggtgat
    21481 gggaaatatc gccgattatt tcaatctgcc tgtttccagt atgagtaaca ccttcacctt
    21541 cctcaacgcc ggcattttaa tctctatctt cctcaacgcc tggctgatgg aaatcgtccc
    21601 gttgaaaacg cagttacgtt ttggctttct cctgatggtg ctggcggttg ccggtttgat
    21661 gttcagccac agcctggcgc tgttctcgac ggcgatgttc attctcgggg tggtcagcgg
    21721 cattactatg tcgattggta cattcctgat aacacaaatg tatgaagggc gtcagcgcgg
    21781 ttcccgcctg ttatttactg actccttctt cagtatggct gggatgattt tcccaatgat
    21841 cgccgcgttt ctactggcgc gcagcattga gtggtactgg gtttatgcct gcatcgggct
    21901 ggtgtatgtc gctattttta ttctgacctt cggctgtgag ttcccggcac tgggcaaacg
    21961 tgcaccaaaa acggatgctc cggtagagaa agaaaagtgg gggatcggcg tactgtttct
    22021 ctctgttgcg gcgctgtgct acatcctcgg tcagttaggt tttatctcct gggtgcctga
    22081 gtatgccaaa ggcctgggca tgagcctgaa cgacgcgggc acgctggtga gtaacttctg
    22141 gatgtcatac atggtcggca tgtgggcgtt cagctttatt cttcgcttct ttgatttgca
    22201 acgcattctg accgtactgg ctggtctggc tgcgattctg atgtacgtct ttaacaccgg
    22261 aacaccagta catatggcgt ggtcaattct cgctctgggc ttcttctcca gcgcgatcta
    22321 taccaccatc atcaccctgg gttcacagca gaccaaagta ccgtcgccaa aactggttaa
    22381 ctttgtcctg acctgcggaa ccatcggtac tatgttgacc tttgtggtta ccggcacgat
    22441 tgttgaacat agcggtccgc aggcggcact gcttacagca aacggtctgt acgctgtcgt
    22501 ctttgtgatg tgcttcctgt taggtttcgt cagccgtcac cgtcagcata acaccctgac
    22561 ctctcattaa ttgctaatgc cggacggcac tatcgtcgtc cggccttttc ctctcttccc
    22621 ccgctacgtg catctatttc tataaacccg ttcattttgt ccattttttg cacaaacatg
    22681 aaatgtcaga caattccgtg acttaagaaa atttatacaa atcagcaata taccccttaa
    22741 ggagtatata aaggtgaatt tgatttacat caataagcgg ggttgctgaa tcgttaaggt
    22801 aggcggtaat agaaaagaaa tcgaggcaaa aatgagcaaa gtcagactcg caattatcgg
    22861 taacggtatg gtcggccatc gctttatcga agatcttctt gataaatctg atgcggccaa
    22921 ctttgatatt accgttttct gtgaagaacc gcgcatcgct tatgaccgcg tacacctctc
    22981 atcttacttc tctcaccaca ccgccgaaga gctgtcgctg gtgcgcgaag gcttctacga
    23041 gaaacacggc atcaaagttc tggtcggcga acgcgctatc accatcaacc gtcaggagaa
    23101 ggtgattcac tccagcgccg gacgtaccgt tttttatgac aagctgatca tggcaaccgg
    23161 ttcctacccg tggatcccac caatcaaagg ttctgatact caggactgct ttgtctatcg
    23221 caccattgaa gacctcaacg ccattgaatc ctgcgcccgt cgcagcaaac gcggtgccgt
    23281 tgttggtggc ggcctgttag gtctggaagc cgctggcgcg ctgaaaaact taggtattga
    23341 aacccacgtt atcgaatttg cccctatgct gatggcggaa cagcttgatc agatgggtgg
    23401 cgagcagctg cgtcgcaaaa tcgaaagtat gggcgtgcgc gttcacacca gcaaaaacac
    23461 ccttgagatt gtgcaggaag gtgtcgaagc gcgtaaaacc atgcgttttg ccgacggcag
    23521 cgaactggaa gtcgacttta tcgtcttctc taccggtatc cgtccgcgcg ataagctggc
    23581 aacccagtgt ggtctggacg ttgctccgcg tggggggatt gtcattaatg attcctgcca
    23641 gacttccgat ccggatatct acgccatcgg tgaatgcgca agctggaaca accgtgtatt
    23701 tggtctggta gcaccaggct acaaaatggc gcaggtcgcc gttgaccata ttctgggtag
    23761 cgaaaacgcc tttgaaggtg ctgaccttag cgccaagctg aaactgctgg gcgtagacgt
    23821 aggtggtatt ggtgatgcgc acggtcgcac gcctggcgca cgtagctatg tttacctcga
    23881 cgaaagtaaa gagatctaca aacgcctgat tgtcagcgaa gacaacaaaa ccctgctcgg
    23941 tgcggtactg gtgggcgata ccagcgacta cggcaacctg ctgcaactgg tgctgaacgc
    24001 tatcgagctg ccggaaaacc cggattctct gattctgccg gcgcactcgg gtagcggtaa
    24061 gccgtctatc ggtgttgata aactgccgga cagcgcgcaa atctgctcct gcttcgacgt
    24121 cactaaaggt gatctgattg ctgccatcaa caaaggctgc cacacagttg cggcgctgaa
    24181 agctgaaacc aaagcgggta ctggctgcgg tggctgtatc ccgctggtca ctcaggtact
    24241 gaacgcggaa ctggcgaaac agggcatcga agttaacaac aacctgtgcg aacactttgc
    24301 ttattcgcgt caggaactgt tccatttgat ccgcgttgaa ggcattaaaa ccttcgaaga
    24361 actgctggcg aaacacggca aaggctacgg ttgtgaagtt tgtaaaccaa ccgtcggttc
    24421 gctgctggcc tcctgctgga acgaatacat tctgaagccg gaacatactc cgctgcagga
    24481 ttctaacgac aacttcctcg ctaacatcca gaaagacggc acctactcgg tgatcccgcg
    24541 ttctccgggc ggtgaaatca ccccggaagg gctgatggca gtgggtcgta tcgcgcgtga
    24601 atttaatctc tacaccaaaa tcaccggctc ccagcgcctg gcgatgtttg gcgcacagaa
    24661 agacgatctg ccggaaatct ggcgtcagct gattgaagct ggcttcgaaa ccggtcatgc
    24721 ctatgcgaaa gcactgcgta tggcgaaaac ctgcgtgggt agcacctggt gccgctacgg
    24781 tgttggcgat agcgtcggcc tcggcgtgga actggaaaac cgctacaaag gcatccgtac
    24841 gccgcacaaa atgaagttcg gtgtctccgg ctgtacccgt gaatgttcag aagctcaggg
    24901 taaagacgtg ggtattatcg ccactgaaaa aggctggaac ctgtatgttt gcggtaacgg
    24961 cggcatgaaa ccgcgtcatg cggatctgct ggcagcggat atcgatcgcg aaacgctgat
    25021 caaatatctc gaccgcttca tgatgttcta catccgtacc gccgacaaac tgacgcgtac
    25081 cgcgccgtgg ttagaaaacc tcgaaggcgg catcgattac ctgaaagcgg tgatcattga
    25141 cgacaaactg gggctgaacg ctcatctgga agaagagatg gcgcgcctgc gtgaagcggt
    25201 agtgtgtgag tggactgaaa cggtcaatac accgtccgcg cagactcgct tcaaacactt
    25261 catcaacagc gacaagcgtg acccgaacgt gcagatggtg ccagagcgcg aacagcaccg
    25321 tccggcaacg ccgtatgaac gtatcccggt aactctggtg gaggacaacg catgagccag
    25381 tggaaagaca tctgcaaaat cgatgacatc ctgcctgaaa ccggcgtctg cgcactgtta
    25441 ggtgacaagc aggtagcgat tttccgcccg tatcacagcg accaggtgtt tgcgatcagc
    25501 aacattgatc cgttcttcga gtccagcgtg ctatcacgcg ggctgattgc ggaacaccag
    25561 ggcgagctgt gggtcgccag cccgctgaaa aaacagcgtt ttcgcttaag tgatggcttg
    25621 tgcatggaag acgaacagtt ttccgtcaaa cattacgaag cacgagtgaa agacggcgtg
    25681 gtgcagctgc gcggttaatg ttttaacggg aggcgcaatg cctccccttt ttgcatggtc
    25741 ctgtaatgac cttcggtata ttgcaggaca ttttttaaac tttttgtttt attttttgtt
    25801 tttatttttt aaaggataat caaatgttta cagacactat taataagtgt gcggctaatg
    25861 ctgcgcgcat tgcacgcctg tcggcaaata acccgctcgg cttttgggtc agctccgcca
    25921 tggcgggcgc atatgtgggt cttgggatca tcctgatttt cacgctcggt aatttgctcg
    25981 acccgtccgt acgtccgctg gtgatgggcg cgacctttgg tatcgcctta acgctggtga
    26041 ttatcgccgg ttctgaactg ttcaccggac acaccatgtt cctcaccttt ggggtaaaag
    26101 cgggcagcat cagccacggg caaatgtggg caatcctgcc gcaaacctgg ctgggtaacc
    26161 tggtcggttc cgtcttcgtt gccatgctct atagctgggg cggcggtagc ctgctgccgg
    26221 tagataccag catcgttcac tccgtcgcgc tggctaaaac cactgcaccg gcaatggtac
    26281 tgttcttcaa aggtgcattg tgtaactggc tggtttgcct ggcaatctgg atggcgctgc
    26341 gcactgaagg ggcggcgaaa tttatcgcta tctggtggtg tctgctggca tttatcgcgt
    26401 ccggctacga gcactctatc gctaacatga cgctgttcgc gctctcctgg ttcggcaacc
    26461 acagcgaagc ctacacgctg gcgggtattg gtcataacct gctgtgggtg acgctgggta
    26521 atactttatc aggtgccgta ttcatgggat tgggttattg gtatgctacg ccgaaagcga
    26581 atcgtccggt tgcggacaaa tttaatcaaa ctgaaacagc tgccggttaa ttactaaggg
    26641 gtttttacgt ggatcatttg cctatatttt gccaattacg cgatcgcgac tgtctgattg
    26701 tcggcggtgg tgatgtcgcg gaacgcaaag caaggttgct gttagacgca ggcgctcgct
    26761 taacggtgaa tgcattagcg tttattccac agttcaccgc atgggcagat gcaggcatgt
    26821 taaccctcgt cgaagggcca tttgatgaaa gccttctcga cacctgctgg ctggcgattg
    26881 cagcgacgga tgatgacacg cttaaccagc gcgtcagcga agccgctgaa gctcgtcgca
    26941 tcttctgtaa cgtagttgat gcgccgaaag ccgccagctt tattatgccg tcgattattg
    27001 accgctcacc gctaatggta gcggtctcct ctggcggcac ctctccggtt ctggcgcgcc
    27061 tgttgcgcga aaaactcgaa tcactgctgc cgttgcatct gggccaggtg gcgaaatatg
    27121 ccgggcaatt acgcgggcga gtgaaacaac agttcgccac gatgagtgag cgtcgccgtt
    27181 tctgggagaa attgttcgtt aacgaccgcc tggcgcagtc gctggcaaac aacgatcaga
    27241 aagccattac tgaaacgacc gaacagttaa tcaacgaacc gctcgaccat cgcggtgaag
    27301 tggtgctggt tggtgcaggt ccgggcgatg ccgggctgct gacgctgaaa ggactgcaac
    27361 aaattcagca ggcagatgtg gtggtctacg accgtctggt ttctgacgat attatgaatc
    27421 tggtacgccg cgatgctgat cgcgttttcg tcggcaaacg cgcgggatac cactgtgtac
    27481 cgcaggaaga gattaaccag atcctgctgc gggaagcgca aaaaggcaaa cgcgtggtgc
    27541 ggctgaaagg tggcgatccg tttatttttg gccgtggtgg cgaagagctg gaaacactgt
    27601 gcaacgcggg tattccgttc tcggtggttc cgggtattac cgcagcttct ggttgctctg
    27661 cctattcggg tattccactc acgcatcgcg attatgccca gagcgtacgc ttaattaccg
    27721 gacacttaaa aaccggtggc gagctggact gggaaaacct ggcggcagaa aaacagacgc
    27781 tggtgttcta tatggggttg aatcaggccg cgactattca gcaaaagctg attgaacacg
    27841 gtatgcctgg cgaaatgccg gtggcaattg tcgaaaacgg aacggcagtc acgcagcgcg
    27901 tgattgacgg tacgctcacg caactcggtg aacttgctca gcaaatgaac agtccatcgc
    27961 taattattat tggtcgggtt gttggcctgc gcgataaact gaactggttc tccaatcatt
    28021 aatttaaccc gggccagaga attctggcct tcttaatgac tctttttata tattcagcaa
    28081 ataaaacata accccttaat aaatattatc tgctaatggg ttatattcac cgttacagcg
    28141 atttacaaaa ttaagtcatt cgctcactga cgccaattaa atattcctgt atcgttctta
    28201 acgagcagtc tgcttatatt catataatca atgaatatta attaatatat aatacatagg
    28261 gaatgtaaat gaacaaattt attaaagttg cactggtagg tgcagtactg gctacgttaa
    28321 ctgcatgtac tggtcatatt gaaaaccgtg ataagaactg ctcttacgac tacctgctgc
    28381 acccggcaat ttctatttct aaaatcattg gcggttgcgg tcctactgca cagtaagtct
    28441 ctgacagaaa tcggctaaca ccggtttaat tgcccgacgc cctctggatt ttccgagggc
    28501 gtatttgttt ctatgtcctt cttgcccccg cccgtaaaaa tattttcact ttaaattcaa
    28561 ttttataact acatccatta gccacagcgc gatcctgtgc gaaattttgt gatcttcctc
    28621 cacatcacat aacataatat gttgttatat tcatcatgca ttgtcatgtt accttttaaa
    28681 tggctgcaaa ctctccccta caacgtattg gacaagaaaa aggtatcgct atgggaagcc
    28741 aggaactcca acgcaagctc ggattttggg ccgttcttgc aatcgccgtc gggacaaccg
    28801 tcggctccgg tatttttgta tctgtgggtg aagtggcaaa agcagcgggc acgccgtggc
    28861 ttacggtgct cgcgtttgtc attggcgggt taattgtgat cccgcaaatg tgcgtctatg
    28921 cggaactatc caccgcttat ccggaaaatg gcgcagatta tgtttatctg aaaaatgccg
    28981 gaagccgacc gctggctttc ctctccggct gggccagctt ctgggccaac gatgcgccgt
    29041 cattgtcgat tatggcgctg gcgattgtca gcaatcttgg ctttttaacg cctatcgatc
    29101 cgttgctcgg taaatttatc gccgccggat taattatcgc ctttatgttg ctacacctgc
    29161 gctccgttga aggcggcgca gcgtttcaga cgctaattac catcgccaaa attatcccgt
    29221 tcactatcgt cattggcctt gggatcttct ggtttaaagc ggagaatttt gccgccctta
    29281 ccaccactgc gattggcgca acgggcagct ttatggcgct gctggcgggg atctctgcca
    29341 ccagttggtc gtataccggc atggcctcta tctgttatat gaccggcgaa attaaaaacc
    29401 ccggaaaaac catgccacga gcgctgattg gttcctgtct gctggttctg gtgctctaca
    29461 ccctgctggc gctggtgatt tccggcctga tgcccttcga caaactcgcc aattctgaaa
    29521 cgccgatttc cgacgccctg acctagatcc ccgcactcgg cagcaccgct gggctctttg
    29581 ttgccatcac ggcgatgatc gtcattcttg gttcgctttc cagctgcgtg atgtaccagc
    29641 cgcggctgga atacgcgatg gcgaaagaca acctgttctt taaatgcttc ggccatgcgc
    29701 atccgaaata caacacgccg gatgtctcca tcatcctgca aggggcgctg gggatcttct
    29761 tcatcttcgt ttccgatctc accagcctgc tgggttattt caccctggtg atgtgtttca
    29821 aaaataccct caccttcggc tccatcatct ggtgtcgtaa acgcgacgat tacaaaccgc
    29881 tgtggcgtac tccggctttc gggctgatga ccaccctcgc cattgcgtca agcctcattc
    29941 tggtcgcctc aacctttgtc tgggcaccga ttcccggcct tatctgcgcc gccatcgtta
    30001 ttgctactgg tctgcctgct tacgccttct gggcgaagcg tagccgccag ctcaacgctt
    30061 tgtcgtaatg ttatctgaag aaaataaaat gttggatatt gataaaagca ccgtggactt
    30121 tctggtcacc gaaaatatgg ttcaggaagt ggaaaaagtt ctcagccatg acgttccgct
    30181 ggtgcacgcc atcgtggaag agatggtgaa gcgcgacatt gatcgtattt atttcgttgc
    30241 ctgcggctcg ccactcaacg cggcgcaaac ggcgaaacat ctggcggatc gcttttccga
    30301 tcttcaggtc tacgccattt ccggctggga gttctgcgat aacaccccgt atcgcctcga
    30361 cgatcgttgc gcggtgattg gcgtttctga ctacggtaaa acggaagagg taatcaaagc
    30421 gctggagctg ggccgggcct gcggagcact cactgcggcg ttcaccaaac gcgcggacag
    30481 cccgattacc tcggcggcgg aatttagcat tgattatcag gccgactgta tctgggaaat
    30541 tcacctgctg ctctgctaca gcgtggtgct ggagatgatc acccgcctcg cgccgaacgc
    30601 ggaaatcggc aagatcaaaa acgatctcaa gcagttgccg aatgcgctcg gtcatctggt
    30661 acgcacctgg gaagaaaaag gccgccagct tggtgaactg gccagccagt ggccgatgat
    30721 ttataccgtt gctgcgggtc cgctgcgtcc gctgggttac aaagaaggca ttgtaacgct
    30781 gatggaattt acctggacgc acggctgcgt gattgagagc ggagagttcc gccatggccc
    30841 gctggagatt gtcgaaccgg gcgttccgtt cctgttcctg ctcggcaatg atgaaagtcg
    30901 ccacaccacc gaacgcgcca ttaactttgt taaacagcgt actgacaacg tgatcgtcat
    30961 cgattacgcc gaaatttcgc aagggctgca cccgtggctg gcaccgttcc tgatgttcgt
    31021 gccaatggag tggctctgct actacctgtc tatttacaaa gatcacaacc cggatgaacg
    31081 ccgctattac ggtggtctgg tggaatatta atccctctcc cggcccggca acgggccgga
    31141 tttacgcaag gagttacccg atgaaaacag gtatgtttac ctgcggccac cagcggctgc
    31201 ctattgaaca cgcatttcgt gatgcaagcg agctgggtta cgacggcatc gaaatttggg
    31261 gcggtcgccc gcacgcgttc gcgccggact taaaagcggg cggcatcaaa caaatcaagg
    31321 cgctggcgca gacgtatcag atgccgatta tcggttacac gccagaaacc aacggctatc
    31381 cgtataacat gatgctgggc gatgaacata tgcgtcgtga aagcctggac atgatcaagc
    31441 tggcgataga tatggcaaaa gagatgaacg cgggttatac gctgatttct gcggcccacg
    31501 cgggctatct cacgccgcct aacgttatct gggggcggct ggcagagaac ttaagcgaac
    31561 tgtgtgagta cgcggaaaac atcggcatgg atctgatcct cgaaccgtta acgccgtatg
    31621 aatcgaacgt cgtgtgtaat gctaatgatg tgcttcatgc gctggcgctg gtgccttcgc
    31681 cgcgcctgtt cagcatggtc gacatttgcg cgccgtatgt ccaggcggaa ccggtgatga
    31741 gttatttcga caaactgggc gataaattac gtcatctgca tattgtcgac agcgacgggg
    31801 ccagcgacac gcattacatt cctggagaag gcaaaatgcc gctgcgggaa ctgatgcgcg
    31861 atattattga tcggggctat gagggttact ctacggtgga gctggtgacg atgtatatga
    31921 acgagcccag actctatgcc cgccaggcgc tggaacgctt tcgcgcgctg ctgccggagg
    31981 atgagagatg aaaaccctgg cgacaatcgg cgataactgc gtcgatatct acccgcaact
    32041 gaataaagcg ttttctggcg gtaatgcggt caatgtggcg gtgtactgca ctcgctacgg
    32101 catacagccg ggatgcatta cctgggtggg tgacgatgac tacggcacaa agctgaagca
    32161 ggatctcgcc cgcatgggcg tcgatatcag ccatgtccat acgaaacacg gcgttaccgc
    32221 acaaactcag gtggaactgc acgacaatga tcgcgttttt ggcgactaca ccgaaggcgt
    32281 gatggccgac tttgccctga gtgaagagga ttacgcctgg ctggcgcagt atgacattgt
    32341 gcacgcggca atctggggac atgcggaaga cgcattccca cagctgcacg ctgcgggcaa
    32401 acttaccgct ttcgacttct ccgacaagtg ggacagcccg ctctggcaga cactggtgcc
    32461 gcatctcgat tttgcctttg cctccgcacc gcaagaagac gaagcgctgc gtctgaagat
    32521 gaaagcgatt gttgcccgtg gcgcaggaac agtgattgtc acgctgggtg aaaacggcag
    32581 cattgcctgg gatggcgcgc agttctggcg tcaggctcct gaaccggtga cggttatcga
    32641 caccatgggt gccggagatt cgttcattgc cggattcctt tgcggctggt ctgcggggat
    32701 gacattaccg caggcgatgg cgcagggaac ggcgtgcgcg gcgaaaacca ttcagtacca
    32761 cggtgcctgg taggtataac gttggcgtga gcatcttcac gccaacgtgc tgttacttgc
    32821 cggaaaacga ccctataatc cgagtaattc attctttatt tcagggtcga ttatgtcagc
    32881 tacggaccgc tactctcatc aactcctcta cgctaccgtc cgccagcggc tgctggatga
    32941 tatcgcgcag ggggtttacc aggccgggca acagatccct accgaaaacg agctttgtac
    33001 acaatataac gtcagccgca ttaccattcg caaagccatc agcgacttag tggcagacgg
    33061 cgtactgatc cgctggcagg gaaaaggcac ctttgtacaa agccagaaag ttgaaaacgc
    33121 cctgcttact gtcagtggtt ttaccgattt tggcgtctca caaggcaagt cgacgaaaga
    33181 gaaagtgatc gaacaggaac gggtcagcgc cgcgccgttt tgcgaaaagc tgaacatccc
    33241 cggaaacagc gaagtgttcc atctctgccg ggtgatgtat ctcgataaag agccgctgtt
    33301 tattgatagt tcatggatcc cgctgtcgcg ttatcctgac tttgatgaga tttacgtcga
    33361 aggaagctcc acctatcagt tatttcagga gcgttttgac acgcgagtgg tcagcgacaa
    33421 aaagaccatc gatatctttg ccgccacccg cccgcaggca aaatggctga aatgcgaact
    33481 gggcgaaccg ttgtttcgca tcagcaaaat cgcctttgat cagaatgaca aaccggtgca
    33541 cgtctccgaa ctcttctgcc gcgccaatcg catcacctta acgattgata ataaaagaca
    33601 ttaaccgtag gccggataag atgcgccagc atcgcatccg gcgatgctgg cgcactgaat
    33661 tttacatccc gcacgttccc ctcaccctaa ccctctcccc aaaggggcga ggggaccgtt
    33721 cagtgcggaa tttagcttga ttgccgaacg gtgctatcca gcattaaata ctcgcaatac
    33781 tctcacgcaa aatccgcagc accgtctctt caccgctgcg attcgggtta atgcgaatcg
    33841 cacaatgttc gagttgtgga ttcgcctggc gaaacgttcc tgaaagtcga tagaagagcg
    33901 gcgggatttc atatttcgac tctgcaccga ccgggtaagg caacgcgcca cgcttttgcg
    33961 cttcttccag caccctggcg gcaatcagct gatgaaactc gacaatcaac accttcgact
    34021 gcgcattggc aatgacggcg cttttcactt ccgccaccgc gccgccgtta agcaaagcca
    34081 gcaaccgttc ggatactccc gcctgcaccg catgcatcac tggcgcgaac accagaccac
    34141 gcaagacttc cagcgcctgt gagccctgga tctggctacc gccggagtaa agcgtggcgc
    34201 gaatccgact aataacatcg gcatcgccaa ccactgcacc aacgccctcc ggcccaaaca
    34261 gcttgaagca ggagaatgtc gagacattcg cgccgcattc acagccgatt cgcgctacct
    34321 tcatcaccgc atagttatca tcggttaacg ctggaacacc tgccgcgcgc aacgttgcca
    34381 gcacatctgc cagcacatag ctgtcctgcg gctgctggcg cgtatgctgc accagcgcgg
    34441 catcaggttg ttgctcgtcg acgacctgct tcagtgccga caggtcattg aaatcagcag
    34501 taataagcgt cagccccatc tgctcaataa taacccgtgt cgtcgggtaa acaggcgcgt
    34561 catgcaccag aagacgctgc cccggtttga gcaaagccgc cagcccggcg cgaatcgcgc
    34621 cagtccccgc gccctgcacc agcgccgcgg cctgtgcgtg aaatgcatca gccagcacct
    34681 gctccacccg ttgggtaata cgcggttgat tcagacttgg agttaaccct aaatcaccgc
    34741 cagcaagaaa ctcgctgcct ggaaaatggc ggcaaatagt gtccaccagc gcaaactgct
    34801 tttgctgcgc ttcagcaaga gtcaggcttt gcaaaggaaa cgtcttcata ggtgccttcc
    34861 ttaagccgga acgaacaggc caagccagta aagaacattc agcagaatac cggtgatcat
    34921 tactgccacg accggtgccg ccattttctg taccggacgg cccagggatt cgttgaggaa
    34981 gtaaatggca accgcgatag agaatccggt ataacccgcc atcttaattg ctgcgaaaat
    35041 cgaaccgacc agcagcgcca cttccatcag catattcatg gcgttacgga tgttatccga
    35101 cgcattacgc accgacgggt agcgtcccag ccatttgccg atcgaacgaa gcagcaagac
    35161 ttccgccgaa ataaccactg cgcctaatac cgctgcaacc atcggattcg gcgagagata
    35221 gcccaccgca taaacaaagg taaagcccgc aactgcatac acacccgttg ctaaggcggt
    35281 ggtggcaatc aacggcacaa accccagtcc gcgcataaat tctgccagag ccgcctgatt
    35341 aatcagcgtt tgcgattgtt ccggcgtcac gcctgcggaa tacgctttct ccagtgtgaa
    35401 gatcgaaact tcgctaccag caaaaatctt catactggca acggcggcaa tcaatgctcc
    35461 cacgatggcg atataaggta agtttttgat aatccgcgac gtgcgctctt caaacaccga
    35521 aagcccgctg gcatcgatgt cattttcatc acgatgacgc aggtcgtgag ttatcgcgat
    35581 ccccagcagc atcaccatgc caataaagat ttcgatggat tcagggttaa gatgtgggaa
    35641 atagcgcacg actaccacac gggtcatcag aacaaccacg gcggcgacca gactttgctt
    35701 ccagccaaac tggtagaaaa tcgccaccag cgggaacagt gcaaaagcag aaaccaccgg
    35761 cgagcttaat tccccgaggc tacctaatac atccaccggc agcgcggtca gcagctggtt
    35821 aactggcaac aggcaagtaa ggatcaacac gccccagata gcgccaagac caaacgccat
    35881 caggctgttt atcgccagta cgccgaggat atcggtagga agaaagagta accatgcgtt
    35941 gagcagcccg gttttcaggg taaaagagat ccccaccgag gccacaaaac caatgctcag
    36001 accaaaagcg atactccccg cctcgcgacg gttcatatag ccttcaatca gttgcggcag
    36061 gatcgggtgg atgccgtcat gaaaaacagc cgccgagcga tgctccagaa gcgatgtcat
    36121 acccgtcagg cacgccacca cgataatctg aatatacaga tccatagtcg tgccttttat
    36181 ttcagatggt taatcaacat cggaatcgcg tgttcgacgt gctcaacgga aaggccaaac
    36241 gccactttcc cttcagcgat cattttggcg atatgctcgt ctttcgcttt aatgcctggt
    36301 ttggcaatgg tgcaactttt gttatagcca atcaccgcga tggcaatcga caatgccgcg
    36361 cccgcaccgg tattacacgc gccaatgtaa taatctagtt gcccggattt caccttcatt
    36421 gccgcttcca tgtcgttgtg aatgaaaact tcaaaacagc caggagccgt cgcttcaatg
    36481 gtttttttaa tctgctcacg ctgtaagcct gcaacgccaa tctttttcat gagtctgtcc
    36541 ttattggaaa aattgagagg gattttcacg taacatcaca tccacatcgg cctgactgaa
    36601 tcctgactgg cgcaattgcg gaataaaggt ggtcaataaa tagtcatagc cataaccacc
    36661 attggctttt aaatgggagc ggcgcgtaat atccatcgac agcatgacgc ggttcagcaa
    36721 cccacggtca cgtagcgcgt ggagcatcgc aatgcgcttt tcgtccgggt agtaactgtt
    36781 cttaccaatg gtgtcgaact gcacgtacgc gccgagatcg atcatcttca aaatgttgtc
    36841 gaggttgtct ttcagatcgc agtgaccaac ggtgacgcgc gaaagatcaa ctccgtgggc
    36901 ttgcaacaga gccagttgtt ccagccccat ggtgctgaac gacgtatgcg tggagatcgg
    36961 gcgtccggtc tggttatgcg ccagcgcagc ggcaataaat accttctctt ccagcggcgt
    37021 aatctttcct tcgctggagc cgatctccgc gatgatcccg gctttcagct ccgtgccatc
    37081 gataccctgt tcaatttcat cgaccatctc ctgcgccagt tcctgcacgc tgcgggtcac
    37141 cacatgttcc gggaaaaacg catcctggta ataaccagta caggccacca cgttgatccc
    37201 ggtttcgcgc attacatcga gcataaattg cgcattgcgc cccatgtaac ggttggtcat
    37261 ctcaatcaca ttacgtacac cccgggtcat caggtcgttc atctcctggc aaatgaacgc
    37321 atactgatca aggcggcagt ccacgttgtt tttaaagccg gagagatcaa tatgcagatg
    37381 ctcatgggcc agggtgtaac ccgtcggatc aaaactcata gggtgtctcc tgcaaaccgg
    37441 agggaggaaa gaaaagagcg accattttgt ggcggtggcg cacggaaaaa ttcacacacg
    37501 gtagctccca catcagaaag cgtggtgcgc acaccgagct gcgtggcgac cagcccttgc
    37561 tgataaacca gcactggcac cacttcacgg gtatggtggc tgtgaccaat ggtcggatcg
    37621 ttaccgtgat ccgccatcac gaccaggcaa tcatctggct gcatcgcctc aacaagccgg
    37681 gcaaggttac ggtcaacgac ctgcaaacgt tcggcataac gtgcgacgtc ttctgcatga
    37741 ccagcgaggt cggtttcctg gatattgatg caaataaacg ccgtcggatg ggtgttaaat
    37801 tcgtcgaggg tgatatccat aatccgctgg ctatccacca ggttttgcca gctcacacca
    37861 taaggattgc tgacgatatc tgccacctta cccaccagca cggttggcac gcccgcttca
    37921 tacagttttt gcggcacctg cactttttca tcaacaccgt agcccatatg cacgacctgg
    37981 aagccgttgt cataagcgcc agaacgcggc gcattaatac caataaagcg cccttctttg
    38041 ctttctgcgg catcgagaat acgctgactg ttggttaaca ggccgccaaa tgtaatgacc
    38101 cgaccgacct gtacctggtc acgcacgata cgaccaattt tgattgcgtc gtcaaaagag
    38161 atcacagaga gattggcggt aatgttatag acttggccta aatccgcctc gagattatcg
    38221 ccaatcgcaa ccgcctgatt gaccgacaga aattgcagat catcgccacg gcgctccacc
    38281 tgccagccag cggaaactaa tgcctgctca acacggtcaa tcacatcgcg aaaaggcatc
    38341 cgcagcggcg gtaacgggcg cgtgcctaaa atttcctgat gccccataaa ggtatcgcca
    38401 ccttcatgtt gcagctctgc cacgccccag gttgcggaat ctgacggctg catatcgcct
    38461 ggcgcataac ccaatgtgtt gattagcccc agcttctcca gcgttggtag ctgcaaatgc
    38521 ggcaactggc tcaggatgtg accacaggta ttcgccccgg catcttgcgg gcgcaccagc
    38581 gtgacatctt tcattgcccc tacgccaaag ctatcaatca ctaacaccac aaatcgcgcc
    38641 atttatgcct ccagagggtt gccgaggctg tcataacgac caactatttc tggttcgccg
    38701 cgctgaatac cggacaccag caccacatcg ctacgggtga caaaaatctg cgtgcgaaaa
    38761 cagagcacca ctgcactgct caccggatac tcgcctgcca gcggcagggt gtagtcgata
    38821 ctgctgtcat ccacagtttt gagattggtt tcagtaatct tttgattttc tggcgtaaac
    38881 accagcgcat gttgcgcatg accacgacgg tagtaaccgc cgccgtagca gtagctgtcg
    38941 ccacggaaat gatgggagat ttcacttaac cagagcatcg cgatacgttc aggctgatcg
    39001 ccctgctggt ttgccggaat agtaccggtc agcgcatgac cgggttcggc atgagtcgca
    39061 ccgtattccg ccagcaatgg cagcgaagtg cagctggtcg ctgaaggtac gttcagttgc
    39121 tcaatcgcaa taccagattt cgccagttga tcccgtgctt gtaccagcgt gtgaagattc
    39181 ggtgttggca aaactttccc agcagcctca tcccaaagca ggcaagggaa atgggttagt
    39241 ccggctaaat gcagccccgg cagtttctta atttcagcaa tcacctcgtg cagcgaatgc
    39301 tgtacaaaac cgctctcctg ccccggataa agaaaatcat cgtcgctata aactttgagc
    39361 agcacaggct ggacccgtcc ggccttaacc gctgccgcag aaacttcccg cgctttgtcg
    39421 agagtaaaca cggtgatgat gtcggtgccc tgttcaacgg cgtcagcaac ctgatgacaa
    39481 gggatttgta ccagatgccc ctgatgcgcc actggcaaac cagcgcggcg catgactcgc
    39541 gcctctttgt aatccaccgc cacaatgccg ctgtagccta atgccagcaa tttttccgcc
    39601 agccacggat tacgaccaaa ttgcttggtc atcagataca gttcaatgcc gtaaagccgc
    39661 gccgtctcaa tgagccgctt accgttttcc agtatctgat ccacgtcgat cacccagctg
    39721 tccggggcga ttttgccctg ctgccacaaa cttagtgcgg cggaaatcag cgccgggttc
    39781 tggcgtttca atgcttctac aaacatcttg ttgcctcgtt tctgttcctt gaattaaata
    39841 ttcatttttt tgaatattta ggttgtgcga cgtggatttg tcgggagctt caaacctctt
    39901 cgttagcagc catccataat ccataaagat tggccagaaa atagccttct tcacacgggt
    39961 taacttccag agcgaattcc ttcaacaact cctgatgcaa ttgcacaacg gcttgccagt
    40021 ggctggattg cgccagttcc gccagcaact cgttatccag cggctctatt tcttcaccac
    40081 ggcgactgcg catcagtgca ctcgccatat gtgtcatcgc catcgttcct tgctcactgc
    40141 gcaccggcag atggcactct ttttccagta cgttgacgac ctgcatcatg cctttgcaga
    40201 cgtccttatc aataacgcct gcctcgcaaa gcaggttgag tctggtttcc atatcagatg
    40261 cctttcgttt aataactggg ttcctgttcc ccactcacca cccgttgttg atgggcaagc
    40321 agggcgtgtt tatctacaac agcacgcagc aattgttgca ttgggtaatc atcgactcgc
    40381 gtcaggacca cggcttcggt ggcctgtaaa aatcgcggat cgtctgttag cggcgtcgcc
    40441 tctaatccca gcatggtcag ttcgttttcc gccaccacgt tccagatcac cgcatcgaca
    40501 tcgcctttaa caatgcgttg taaactctcg tgataagaga gatcgactcg ttccacatca
    40561 ctatcgccaa aaaagacatc ggtcatgatt ttctgatccg ccgaacgact atcgagacct
    40621 actcgcttaa catttccaga ctcacctttg cggcatatca gttgatgttc accaacatac
    40681 gtatgcgggc caagctcgag ggcgatgcac aaattgtttt ggctgagata actttccgcc
    40741 gccaggcggg aaactactgc catgtcatag acaccgttaa gtaaacactc cacgcgaata
    40801 tccgcgccgc gcatatgcgc atagtaaaaa ggaatgccat caaactgggc tttcaatccg
    40861 ctcgctaaac cttcgtacaa gcgggtatag ggcaaaggca tcgcacatac cacgttgttg
    40921 atatccacat gactcagcaa tgctttgtta tccatctcga caagataact gccattgcgc
    40981 ccacggcgtt caatccgtat cgctccgctt gattccagcg ttttcaacgc ggcctgcgtc
    41041 aggccaaccg atgaacggca ttcagttgcc agctcatcta tggttttcag tcgattaccg
    41101 catttttcac ccaacaaata acgggccagt gtcgtgatga cgacgccttc ttttttgata
    41161 aacgttcgac gcataatata ttttcaataa aatgaatatt tatatcttca ttaaaatgaa
    41221 gataagtggc gacgacgaga tgtggaaacg gcgaggcgct tcacattttt tcgggttgtt
    41281 gcgaaaagag gataaaaaaa ccgggtttcc ccggtttcag agtgatgacg aagtgattta
    41341 ttcgacaatg cgagcaaagg gtaattccca ctggcgcttg cctgctcccc tcaccctaac
    41401 cctctcccca aaggggcgag gggaccgtcc gagcgttttg atattacggc ttcgccacaa
    41461 aaccaatcgc ttcgtacacc gctttcagcg tacgggaagc gtgtacactg gctttttctg
    41521 cgccgtcttt catcacctgt tgcaggaagg cttcatcgtt gcggaaacgg tgatagcgtt
    41581 cctgcaattc agtcagcata ccggaaacgg catcagccac ttcacctttc agatgaccat
    41641 acatcttgcc ttcgaactgt ttttccagtt ctgggatgct ctggcccgtt accgcagaaa
    41701 ggatatccag caggttggaa acgcccgctt tgttctgcac atcgtagcga actaccggcg
    41761 gctcgtcgga gtcagtgacc gcgcgtttga ttttcttcac taccgatttc ggatcttcca
    41821 gcaggccgat aacgttattg cggttatcgt cagacttgga catcttcttg gtcggctcca
    41881 gcagcgacat tacgcgcgcg ccagatttcg gaataaacgg ctccggcacc ttaaagatct
    41941 cgccatacag cgcgttgaaa cgctgggcga tatcgcgact cagttccagg tgctgtttct
    42001 ggtcttcacc caccggtacc agattagttt gatacagcag gatgtccgca gccatcagca
    42061 ccggatagcc aaacagacca gcgttgatgt tctcggcata acgcgcagat ttatctttaa
    42121 actgcgtcat gcgactcagt tcgccgaagt aggtatagca gttcagtgcc cagcctaact
    42181 gcgcatgttc tggtacgtgg gactgaacaa aaatggtgct tttctcagga tcgataccac
    42241 aagccagata caaggccagc gtatccaacg tcgctttacg cagcttctgt gcatcctggc
    42301 gcacggtgat cgcgtgttgg tcaacgatac agtaaatgca atggtagtca tcctgcatgt
    42361 ttacccactg acgcagcgca cccatgtagt taccaatggt caattcacct gagggctgtg
    42421 cgccactaaa aacgatgggc ttagtcattt ttcgattcct gattttcgct atgcggaagc
    42481 cctaatgcgg gcagaaggtc atttatagac tgataaatta catcaggctg gctgagatcg
    42541 atagcctcgc cgtagttgta tccgtaggtt aagccaactg atgggcaacc tgccgctttt
    42601 gccgcctgaa tatcattccg tgagtcgccg acaaacagca tctgttgtgg ggcaattccc
    42661 atccgctcag ccaccagtaa cagcgggtcc ggatgcggtt ttttgttttg cacatcatcg
    42721 ccgccgataa ccacgctaaa gtatttggca atatctaagg cttcgagcag cggcgcgacg
    42781 aacggcgtcg gtttgttggt gaccaagcct agcggcaggc ctttagcctg caacgcgccc
    42841 aacgtatcgg caacgtgcgg gaacaaaaac gtcccctctt cggcaacctc gccatagtag
    42901 cgatcgaaca gtttacgcag aatacgtacc tgttcttctg ccggaatgtc gtcatcaacg
    42961 ggcggtttcc ccattgtttt acgcagagtc gcacgttcct gacgcgccca ggtcaatgcg
    43021 cgctccatca gaacatctgc gccgttacca atccaggtaa taacgcgttc ttcacctgcg
    43081 acgggcaact ccagcgcata cagcgccata tctaccgcag cagcaagacc aggagcactg
    43141 tcgaccagcg taccatcaag atcaaaagcg acgccgcgaa tatcttcaaa cttattcatg
    43201 acttaccttt gccagttcac tgcgcatttc atcaatgact tttttatagt ctggctggtc
    43261 gaagattgcc gaaccggcga cgaacatatc cgcgcccgcc gcagcgattt cgccaatgtt
    43321 gttcaccttc acgccaccgt ccacttccag acgaatgtca aagccagact cgtcgatacg
    43381 gcgacgtact tcgcgcagtt tatccagtgt ttgaggaatg aaagactgac cgccgaaacc
    43441 agggttgacg gacatcagca ggatcacatc cagcttatcc atcacgtaat ccagatagct
    43501 cagaggtgtc gccgggttaa ataccagacc cgctttacag ccattttctt taatcagttg
    43561 cagcgtgcgg tcaacatgct cggaggcttc tggatgaaag gtaatgatgc tggcaccagc
    43621 ggcagcgaaa tcaggcacaa tgcgatcgac gggtttcacc atcagatgta cgtcgatagg
    43681 ggcggtaatg ccgtagttac gcaaggattt cagcaccatt ggcccaatcg tcagattggg
    43741 aacatagtgg ttatccatga cgtcaaaatg cacgacatca gcgccagctg ccagggcttt
    43801 tgcggtatct tcacccaggc gggcaaaatc agccgacaga attgaggggg caatcaaata
    43861 ctgtttcatc cgcttctcct tgagaattat tttttcgcgg gtgaaacgac tcctggtttg
    43921 tacaaagcca gcagttcgtc caccttttta cgtgtgccgc cgttgctgct tatactgcgt
    43981 cgaactttga cgacatgcaa ttttgcgcgc tgataccact cacgcgttaa catcgtatcg
    44041 tgattggaga tcagcactgg aatatggcgc tcaaccagac cttcggcgat ctccgccaga
    44101 tgcgcttgtt gttcaagcgt aaaactgttt gtgtgatacg ccgtaaagtt ggcggtcgca
    44161 gacagcggtg cataaggcgg atcgcaatag acgacggatg catcatctgc gcgctccatg
    44221 ctatcggcgt aagactcaca atagaaaaag gcattctgcg ctttttcagc gaagtgatac
    44281 aactctgctt ccgggaaata gggttttttg tagcggccaa acggcacgtt aaactcaccg
    44341 cgcagattgt aacgacacag gccgttgtaa ccgtagcggt tcaaatataa aaacagtacc
    44401 gcccgacgga acggatcctg gcttttgttg aactcttcgc ggaactgata gtaaacctcg
    44461 gcgcaatttg tttcgggaac aaacagctcg cgtgcggcct gtacgtactc atcagtacgc
    44521 atcttcacaa tgttatagag actgatcagg tcgctattga tatcggcaag gatataacga
    44581 gaaaagtcgg tgttgagaaa caccgacccg gcacctacaa aaggctcaac cagacattcg
    44641 cccttgggca aatgccgttt aatatcatca agcaggggat acttgccccc tgcccacttc
    44701 aaaaaagcgc gatttttctt catgccgatt aactaattac accttctccg gctgtggaga
    44761 aagctccgac agcatccagc gcttcaggca gctcccgcaa cgataaacca ttgcgggaga
    44821 taaccttgat tacttcagat cggcctgtac ctgacgcagc ggtttcgccc acgggttttt
    44881 ggcctgaaca tctgctggca atgtagatac cgcttttttc gcctcttctt tcgaagcgta
    44941 cacgccagaa accaggacat accacggctg accattacgc gtcgtttcat agacaacgta
    45001 gtttttcaga ttctctttct tcgcccaacc gttcaggttg tcgtagttag aggaactgct
    45061 cagctgcaga gtgtaatggc tggacggtgc cgatttcaac gaaccaacat tacctgcggt
    45121 cttccctcca gcggctggtg ttgccgtggt ttgcgccggg gatgccgtct gtactggagc
    45181 cgtagtcgcc gtctcttttg gtgctggcgt agaagtcgca gccggtgcct tcgtgctcgc
    45241 cacaggggca gctggttcag gttcagtacg cttcggcgtc tgtgctaccg gcttcggctc
    45301 cgttttcacg gttgcttgcg gttttttcgg ttcaatcacc gcctgctgac gaactgggcg
    45361 cgtagtggac ggacgttcag cggtttgcgt tttcgccgtg tcacgcgatg cattgccatt
    45421 gcgaacaggc gcaaccgttg cgggttcagt cggcaacgtg gaattgaccg ccacattgtt
    45481 cagctgttgc tgattttgtg gctgggtcag cgcattgttc aggtcaccct gcacttcaac
    45541 acgttgttga ccatccgttg ccaccggggt ttgcccttga gtcggcgtag aagagatcgg
    45601 tggcagagaa acatcctgct gagtattttc cgcagacgtg gttcccggcg ctggctgcac
    45661 gccattcgcc tgatcggtcg cattgccagc aagatcaata ctcttctcgc cagacgcggt
    45721 ttgatcgctg gaagtggtcg agggggcttt tagcgcagaa ccgataccga tgatcaacag
    45781 cagtagaacc agaatgccga cgcccatcat catatactga cgagaagcgg gtttactggc
    45841 tgcttttttg cgcttacgcg gacgacgctc tacgcgctct tcatccacgg tttcatcttc
    45901 ggattcgtca atttcttctt cgatttccgg ttcctcattg cgctcttttt gcgcacgagt
    45961 cggacggcga tcgtcagtgt catcaagttc aatatcatca aaattgatct gcggttcgcc
    46021 acgttcagta cgctcagaac gttcagaaga ttgacgagaa cgaccagtac gacgatcgct
    46081 gggatcgggt ttcagctcgt cttctggttt gaattcatcc atttaacacc ccactaaaag
    46141 gttaatgctt accacgttgc aattaacctg aagctaatag accgcttgat aagcggcctg
    46201 acctttcttg ttgttacgct gattgacaat cggcaatggc gttaagaaca agctcgtgcg
    46261 aaacgccgct gcgaacttca ctcttaccaa ttgccaacgg aagaattaag cgcatctctc
    46321 ccgcaaggac tttcttgtca cgcagcatat gcggtaaata cgcctgcgcg gacatttcgc
    46381 gcggcccatt gaccggtaac ccagcccgcg tgagcagggt tataatacgc tgcgtttcgg
    46441 cagaactaaa ctgcccgaga cgttccgacg tccgcgccgc catcaccata cccgcagcga
    46501 ccgcttcacc atgtaaccaa ttgccatacc ccatttcagc ttcaatggca tgaccaaagg
    46561 tgtgtcccag attcagtaaa gcacgtaacc cggtttcgcg ctcgtcggcg gcgacaactt
    46621 ctgccttcag ttcacaacaa cggcgaatac agtacgccat tgccggaccg tccagacgca
    46681 acaacgcatc cagattctct tccagccagt taaaaaacgc accgtcaaga ataatgccgt
    46741 atttgatgac ttctgccagc cccgacgcta actcacgcgg gggaagcgtt ttcagacagt
    46801 cgagatccac caccactgaa gcaggctggt agaacgcgcc aatcatgttt ttaccgaggg
    46861 gatggttgac cgcagttttg ccgccaacgg aggaatcgac ctgcgacagt aacgtcgtcg
    46921 ggacttgaat gaaacgaaca ccgcgctgat aactcgccgc cgcgaagccg gtcagatcgc
    46981 ccactacgcc gccgccaagc gccaccagcg tagtatcgcg accatgcggc ttttgtaaca
    47041 acgccgtaaa gacggtatcg agtacagcta ggcttttata ctgctcgccg tcagggagga
    47101 taacgctatc gacgttaaca cccgcctgtt caagtacgcc gcggacctta tcgagataca
    47161 gaggagccag ggtttcgctg gtgaccaaca tgacctgctc gcccgatttc agcggtaaga
    47221 atgaagctgg ttcattaaac aaaccagatg cgatggtaat tgggtaacta cgttccccga
    47281 gagtaacgac aatcctctcc ataacgcgac atccacctta attactgtac ccgcagacga
    47341 gtgtatataa agccagaatt agttgctttc cagcatgtga ataatctggt ttgcaaccac
    47401 tttagcgctt tgatcatcag tacgaatggt cacgtcggca atctcttcat acagcggatt
    47461 gcgttcattg gccaacgctt ccagaacttc acgcggcggt gtttcaacgt gcagcaacgg
    47521 gcgtttttta tcacgctgcg tgcgtgcaag ttgcttttcg atggtcgttt caagataaac
    47581 gacaacgcca cgagcggaaa gacggttacg cgtttcacgg gatttcacag agccgccgcc
    47641 agtagccagc acaataccct gtttctcggt caactcattg atgacctttt cttcgcgatc
    47701 gcggaagcct tcttcgcctt ctaaatcgaa aacccagccc acatcagctc cggttcgttt
    47761 ctcaatctct tgatcggaat cgtaaaattc catattgagt tgttgagcta actggcgccc
    47821 aatagtgctt tttccggcac ccataggccc aaccagaaag atattgcgtt tctctgccat
    47881 tttttcggta ctactaagac tattcgttaa tgataaaccc gcttcgctca gagagcgccg
    47941 caggacatga actgaaacct cataagatat tgcgagagtc agactgaaaa ttatctcaat
    48001 actccagcgg gtttggcaac tgaataaatc acccatccct ctgcatatct ggtcgctgca
    48061 agcgcgctgc cttgctacca ccgctctggc gataaatcac cgccgggtaa gattagcgta
    48121 aaaaagacag caaaatgccg cctgaatgat aaatcatcat catggaacgc aggcagcgaa
    48181 gagcaccaac tctcaaatcg gtactccttg tatgctaaat acctgcgcgc gtcaaataga
    48241 tgaaacacgt tcagcataaa aacattaccg cttttacggc tgtttactca ctggaaacca
    48301 gtcgtggcgt gataaacacc actaactcgc gtcgttcatc ttcttttccg tcatgacgaa
    48361 ataattgccc gaaccaggga atgtcgccaa gcaacggtac gctatcctga cccgatttat
    48421 ttttacgggt aaaaatgccg cccagcgcca acgtttctcc gctttttacc tcgacctgcg
    48481 tttcgatctc ctgcttatca atcgccagca cttcgccatc ggcctgctgt agcacctgcc
    48541 ccggaacgtt ctggctgatg tgtaatttca gccggatgcg acctttttgt aacaccgtgg
    48601 gcgtgacctc catccccagg acggcctctt taaattccac cgacgtcgcg ccactttccc
    48661 cgctggaaac ctgatatgga atttcgctct cctgtttaat gctggcaggc tgaagatgtg
    48721 aggccagcag acgcggactg gcgataatat ccagctgctg tttttgttcg agcgcggaaa
    48781 gctcaagatc cagcaagcgt ccgttgatgc gcccaatgtt aaaaccgaca tgcgttgtcg
    48841 ccgtcgctac ggagaggtcg ctaccaagcg tggtgacttg cccaacgcca ccagcgtgtt
    48901 gcgcatcggc cagcgtccat ttcacgccta actcacgcaa acttttttca ttaatggtga
    48961 caatatgcgc cgacagctca acctgcccga ccggcagatc catttgcgct acccactgtt
    49021 caagcgcgct taacgccgtt ttgttatctc gtagcaaaag gcgattggtg cgtttatcga
    49081 cggtcatact ccctttggca ctcagtagct tctcccccgc tttcgccagt tctcccgcgt
    49141 cggcgtattg cagggttata ctgcgatttt ccagcggcag atttgcctgc gcccgcgcct
    49201 gctccgcctc ctggcgggcg atattgtcat tctgccaggc aatggaatgc actgagagaa
    49261 tgttgccttc ctgccgcgtt atcagtccgg cgcttttcac tacagtttgt agtgcctgct
    49321 tccagggaac atctgttaga tgtaacgaca ccgtaccgct gacgtctggc gacacgacca
    49381 ggttcaactt ctcctgttca gccagcgcct gcaacacctg agctaccgga acgtcatcca
    49441 ccatcagcgt cactttttgc ggctttgccg cctgtacgcc gggtatcagc atcaacagta
    49501 gtgcggctat ccattgcttc atttgtatct ccttgccgtt gccacaacca ttgtggcggt
    49561 tcgcagtttg tcccggtacc cagcgttagt acgtctggcg tcagctgtaa aattgtccag
    49621 ccgttttcca gcacatcgtt ttgctgcacc cgtcgccatt tcttttgccc gtcttttatt
    49681 acaccgatga tgcgctcgcc tcgccctacc atcccctgat agcgccactg gctaagttcg
    49741 ctaatccggc atatagatct tccggcgatt taaaagggtc acgcataccg gttaaaaggc
    49801 acaatgcaat acctgccaac aaccagcgtt taaccctcat gcggcatctc cagttgtagc
    49861 gtgaacaaaa gatcatcacc ttccacgctt aacgaaaaac ggctcacgct gacgttgcgc
    49921 tctgccagcc gtgtaaatgc cgacggcact gcttcccaca gcgttttcaa cgccaactca
    49981 cctccctgcg cggatggatg ccaggaaacc agttgcgcgc cggataactg aaaatccagt
    50041 ggcgaaaagg gcagcgtttt ttcctcgctg aagggagtgg tgtctaccag gcgatacagt
    50101 gcggcccact gctggtgatg acttgcccgt agtcgaatta atgcgtcgcg ctcttcatgg
    50161 tgtgtcgatg atagaaaaat gagcgtaact aacatcagca accagaatgc ccagcaaaac
    50221 tggcggaggc ggggtgatgt ggcgaaccac cagtcaaaga acatgttcat cgctaacctt
    50281 ccttgttaac tgatactcaa attgccagcg tccctgcgca tcctgctgcg tggctccccg
    50341 ctgattgaga tgaaaagacg catcctggcg gagtgacgtt tctagtgcgt ttaacgcggt
    50401 aatgcttgtt gtcagcccct tgatctccag cgttccctgc tgccagctta tcgttgtcag
    50461 ccaggcgtgc tcgggtaaaa gcgccgccag tgcttccagc gcagattgcc agtcgcgggt
    50521 aaattgtcgc tggcgctgcc gttgcgaacg ctgttcgcgt aactgctgtt gctccagcaa
    50581 acgtggcttc gttatctgta agctgcgggc gagttgttgt tccgcctgaa gcaaaactgc
    50641 gtctatgcgc gcttcggcgc tgcctgtcag acgcagtatt agcgttatcc cgacggccag
    50701 cagcagaggc gcaacgaaca tcagcaacca gaaacgcaga aaagcggtcc ggcgttgctg
    50761 tcgccagggc aaaaaattaa ttggcggatt catcagtacg ccttcccaag cgccagcccc
    50821 agcgcgatgg caaagtctcc accaggcggc ggtagcggcg gctgacgaac agaaacggcc
    50881 tcccaggggt caaatccgcc ttcgccacat atcgcgacgc tgtcgggatc aacggataac
    50941 gctgccgcca gctccttcgc gctagtcatc cccaccgcca gtttgcgccc ccagctatag
    51001 cgcgtcgccc acagccactg ttcgttatca cgccaggcca gacattgctg atgagaaggt
    51061 aaaaaaggca ggaatcgctg taatgcactg gcatccgggg tgatcgcact aacatgaaca
    51121 cgcaaccttt ctgccagcgt aagcagcgtt gccagctctt tgctttgcgc ggcagtcacg
    51181 ttataagcgg ggctgagtga gtcttcgctg taatcgaagc gcagggagtc cggatccata
    51241 tccagctcgc gggccatcgt ccctgacagc caggccgttt gctcccgctc accaagggac
    51301 atcgacgggc gcggaaatga ccgctgtaat gtgcgactgg cgggaaacgc caacataatg
    51361 tgatgacgct gcggcagttc gcgactccac ggtaacaacg ttttagccag ctgctgcgca
    51421 tcaacaatcc gcccatcttt gataatgtcg tgcgccagcg gcaaccgcca ccagcgttgc
    51481 aaaaagcatt cttttgcgcc ccgcacgatc gcaaccgctg ccgcttcttg ctgttgtaaa
    51541 tgcaaaccaa tttgccagat cttaaatgtc attgtgatga tctccttatc acccgtcact
    51601 ctgacgggta tatcaatgcg tctggcttgc ctttatacta ccgcgcgttt gtttataaac
    51661 tgcccaaatg aaactaaatg ggaaatttcc agtgaagttc gtaaagtatt ttttgatcct
    51721 tgcagtctgt tgcattctgc tgggagcagg ctcgatttat ggcctatacc gctacatcga
    51781 gccacaactg ccggatgtgg tgacattaaa agatgttcgc ctgcaaattc cgatgcagat
    51841 ttacagcgcc gatggcgagc tgattgctca atacggtgag aaacgtcgta ttccggttac
    51901 gttggatcaa atcccaccgg agatggtgaa agcctttatc gcgacggaag acagccgctt
    51961 ctacgagcat cacggcgttg acccggtggg gatcttccgt gcggcaagtg tggcgctgtt
    52021 ctccggccac gcgtcacaag gggcgagtac cattacccag cagctggcga gaaatttctt
    52081 cctcagtcca gaacgcacga tgatgcgtaa gattaaggaa gtcttcctcg cgattcgcat
    52141 tgaacagttg ctgacgaaag acgaaatcct cgagctttat ctgaacaaga tttaccttgg
    52201 ttaccgcgcc tatggtgtcg gtgctgcggc acaagtctat ttcggtaaaa cggtcgacca
    52261 actgacgctg aacgaaatgg cggtgatagc cgggttgccg aaagcgcctt ccacctttaa
    52321 tccgctctac tcaatggatc gtgccgtcgc gcggcgtaac gtcgtactgt cgcggatgct
    52381 ggatgaaggg tatatcaccc agcaacagtt cgatcagaca cgcactgagg cgattaacgc
    52441 taattatcac gcgccggaga ttgctttctc tgcgccgtac ctgagcgaaa tggtgcgcca
    52501 ggagatgtat aaccgttatg gcgaaagtgc ctatgaagac ggttatcgca tttacaccac
    52561 catcacccgc aaagtgcagc aggccgcgca gcaggcggta cgtaataacg tgctggacta
    52621 cgacatgcgc cacggctatc gcggcccggc aaatgtgctg tggaaagtgg gcgagtcggc
    52681 gtgggataac aacaagatta ccgatacgct gaaggcgctg ccgacctatg gtccgctgct
    52741 gcctgccgca gtcaccagcg ccaatcctca gggagcgacg gcgatgctgg cggacgggtc
    52801 gaccgtcgca ttgagtatgg aaggcgttcg ctgggcgcgt ccttaccgtt cggatactca
    52861 gcagggaccg acgccgcgta aagtgaccga tgttctgcaa acgggtcagc aaatctgggt
    52921 tcgtcaggtt ggcgatgcat ggtggctggc acaagtgccg gaagtgaact cggcgctggt
    52981 gtcgatcaat ccgcaaaatg gtgccgttat ggcgctggtc ggtggctttg atttcaatca
    53041 gagcaagttt aaccgcgcca cccaggcact gcgtcaggtg ggttccaaca tcaaaccgtt
    53101 cctctacacc gcggcgatgg ataaaggtct gacgctggca agtatgttga acgatgtgcc
    53161 aatttctcgc tgggatgcag gtgccggttc tgactggcag ccgaagaact caccaccgca
    53221 gtatgctggt ccaattcgct tacgtcaggg gctgggtcag tcgaaaaacg tggtgatggt
    53281 acgtgcaatg cgggcgatgg gcgtcgacta cgctgcagaa tatctgcaac gcttcggctt
    53341 cccggcgcaa aacattgtcc acaccgaatc gctggcgctg ggttcagcgt ccttcacccc
    53401 aatgcaggtg gcgcgcggct acgcggtcat ggcgaacggc ggcttcctag tggacccgtg
    53461 gtttatcagc aaaattgaaa acgatcaggg cggcgtgatt ttcgaagcga aaccgaaagt
    53521 agcctgcccg gaatgcgata ttccagtgat ttacggtgat acgcagaaat cgaacgtgct
    53581 ggaaaataac gatgttgaag atgtcgctat ctcccgcgag cagcagaatg tttctgtacc
    53641 aatgccgcag ctggagcagg caaatcaggc gttagtggcg aagactggcg cgcaggagta
    53701 cgcaccgcac gtcatcaaca ctccgctggc attcctgatt aagagtgctt tgaacaccaa
    53761 tatctttggt gaaccaggct ggcagggtac cggctggcgt gcaggtcgtg atttgcagcg
    53821 tcgcgatatc ggcgggaaaa ccggtaccac taacagttcg aaagatgcgt ggttctcggg
    53881 ttacggtccg ggcgttgtga cctcggtctg gattggcttt gatgatcacc gtcgtaatct
    53941 cggtcataca acggcttccg gagcgattaa agatcagatc tcaggttacg aaggcggtgc
    54001 caagagtgcc cagcctgcat gggacgctta tatgaaagcc gttcttgaag gtgtgccgga
    54061 gcagccgctg acgccgcctc cgggtattgt gacggtgaat atcgatcgca gcaccgggca
    54121 gttagctaat ggtggcaaca gccgcgaaga gtatttcatc gaaggtacgc agccgacaca
    54181 acaggcggtg cacgaggtgg gaacgaccat tatcgataat ggtgaggcac aggaattgtt
    54241 ctgattaaaa aggcgcttcg gcgccttttc agttggctga caaagtgcat ttgtttatgc
    54301 cggatgcggg tgaacgcgtt atccggctaa caaaatcgtg aaaaatcaat atattgcagg
    54361 aaccccataa gcctgataag cgttgtgcat caggcaaact tcacgcattt acactcgccc
    54421 ctgccctttc aaccattcgc gcacgaggaa cagcgcgctg acattacgcg cttcgttgaa
    54481 gtcagggtct tccagcaaat ccatcatatg cgccagcggc cagcgcacct gtggtagcgg
    54541 ctctggctca tcgccttcca gcgattccgg gtagagatct tgcgcgacca cgatattcat
    54601 tttgctggaa aagtaagacg gtgccatgct gagcttcttc aaaaaagtca gatcgttcgc
    54661 accaaatcca acctcttctt ttagctcgcg gttagcggct tcgtagacgc tttcacccgg
    54721 atcaattaat cctttcgaaa aacctaattc gtaggattca gttcccactg cgtattcgcg
    54781 gatcaggatc aggtgatcgt ccacaatcgg cacaatcatc actgcttccc ggttggttgg
    54841 acgcattcgt tcataaacac gccgcacgcc attgctgaac tccagatcca cgctctcgac
    54901 ggtaaacagt cgggaacggg ctacagtttc aacattcaga atggtgggtt tttgtaatga
    54961 tttgttcatc gtgggatcta tgctgtgaaa tcagcggtta ttgtgcgata tcggacacgc
    55021 tttcggcaat gtgaattgca tgttatttac atttatgtaa cttaataaat aatcgtcctc
    55081 aaatcaaatt aaaagtcaat aggttgaaat aactccagga atttgctgat attccgcctt
    55141 cagagggttt gctatgatca gcggttactg tgatgtgctt aatgatgctc aagttaagct
    55201 ccacgcttgc cgatagccaa ccgcagaatc atgtattgtg tccggtgcga ctgaccacgc
    55261 ctgacagact aagtaagatg gggaaagcat gagcaccatt gtgatttttt tagctgcttt
    55321 gctggcctgc tcactacttg cgggatggct gataaaagtg cgatccagac ggcgtcagct
    55381 gccctggacc aacgccttcg cggatgcgca aacgcgtaaa ctcacacctg aagaacgtag
    55441 cgccgttgaa aattatcttg agagcctgac gcaggtatta caggtgcctg gcccaacgga
    55501 agctagcgcg gcaccgatct ctctggcgct gaatgccgaa agcaacaacg tcatgatgct
    55561 gacacacgct atcacacgtt acggcatctc taccgacgat ccgaataaat ggcgttacta
    55621 cctcgattcg gtagaagtcc acctgccccc cttctgggaa cagtacatca acgatgagaa
    55681 taccgttgaa ctgattcata ccgattcgct gccgttggtt atttcactca acggtcatac
    55741 gctgcaggag tacatgcagg aaacgcgcgg ctatgccttg caacctgttc cgtccacgca
    55801 ggcgtcgatt cgcggggaag agagtgagca aatcgagcta ctcaatattc gcaaagaaac
    55861 gcatgaagaa tatgcgctga gtcgtccgcg cgggctgcgt gaagcgttgc tgatcgtcgc
    55921 ctccttcctg atgttctttt tctgcctgat taccccggat gtatttgttc cgtggctggc
    55981 aggcggcgca ttactgctgc tgggcgcagg tctgtggggg ctattcgcgc ccccggcaaa
    56041 atcctccctg cgagaaattc attgtctgcg cggtacaccc cgtcgttggg gattgtttgg
    56101 cgaaaacgat caggaacaga tcaacaatat ttcactcggt attatcgacc tggtctatcc
    56161 cgcacactgg cagccataca ttgctcagga tctcggtcaa caaaccgata ttgatatcta
    56221 tctcgaccgc cacgtagtgc gtcaggggcg atatctttcg ctgcatgatg aagtaaaaaa
    56281 ctttccgtta cagcactggc tgcgcagtac gattatcgct gcgggctcgc tgctagtgct
    56341 gtttatgctg ttattctgga tcccgctgga tatgccgctg aaattcactc tctcatggat
    56401 gaaaggcgcg cagaccattg aagccaccag cgtaaaacaa ctggctgacg ctggcgtgcg
    56461 ggtaggcgat acattgcgta ttagcggtac gggaatgtgt aatattcgca cttccggaac
    56521 ctggagcgcg aaaaccaatt cacctttttt accgtttgac tgctcgcaga tcatctggaa
    56581 tgacgcccgc tcattaccat taccagaatc tgaactggtc aacaaagcga cggcattgac
    56641 tgaagcagtt aatcgccagc tgcaccctaa accggaagat gaatctcgcg tcagtgcctc
    56701 attacgttca gcgattcaaa aatccggcat ggtattgctt gatgattttg gcgacattgt
    56761 actgaagaca gcggatttat gttctgccaa agatgactgt gtgcgactga aaaatgcgct
    56821 ggtcaatctc ggcaacagta aagactggga cgcgctggta aaacgcgcca acgccgggaa
    56881 gctcgatggc gtgaatgtgt tattacgccc ggtgagtgct gaatcgctgg ataacctggt
    56941 ggcaacctcc accgcgccgt ttatcacgca tgaaacggcg cgagcggcac aatcactaaa
    57001 cagtccggcc cccggcggat tcctgattgt cagcgacgaa ggcagcgatt ttgttgatca
    57061 gccctggcct tcggcatcac tttacgacta cccgccgcaa gaacagtgga acgctttcca
    57121 gaaactggca caaatgctga tgcatacgcc gtttaacgcc gaaggtatcg tcacaaaaat
    57181 cttcactgac gccaatggta cgcagcatat tggccttcat ccgatcccgg atcgttccgg
    57241 cctgtggcgc tatctcagca ccacattgct gctactgacg atgctgggta gcgccattta
    57301 caatggcgta caggcctggc gtcgttacca gcgtcatcgc actcgcatga tggagattca
    57361 ggcctattat gaaagctgcc tgaacccgca actgatcacc ccttcagaaa gccttatcga
    57421 ataacacgtt tgcgcggcag gttatgctac cctgtcgcgc aaattgcttc actctggaga
    57481 tttccctcat gcatatcaac attgcctggc aggacgtaga taccgttctg ctggatatgg
    57541 acggcacgtt gctcgacctc gccttcgata actatttctg gcaaaagctg gtgcctgaaa
    57601 catggggcgc gaaaaacggg gttacgccac aggaagcgat ggaatatatg cgccagcaat
    57661 atcacgacgt acagcatacg ctaaactggt actgtcttga ttactggagt gagcaactgg
    57721 gtctggatat ctgtgcgatg accaccgaga tgggaccgcg cgccgtactg cgtgaagata
    57781 ccattccgtt tcttgaggca ctgaaagcca gcggtaagca gcgaattttg ctcaccaatg
    57841 ctcatccgca caacctggcg gtaaaacttg agcatacggg tctggacgca caccttgatt
    57901 tattactttc cacccacaca tttggttatc cgaaagagga tcagcggtta tggcatgcgg
    57961 tggccgaagc tacgggtctg aaagctgaaa gaacgctgtt tattgatgac agcgaagcga
    58021 ttctcgatgc tgccgcgcaa tttggtattc gttactgcct cggcgtgact aatcctgatt
    58081 ccgggattgc cgagaaacag tatcaacgcc atccgtcact gaatgactac cgccgcctga
    58141 tcccctcgct aatgtgaagg agacgccatg aaagagaaac ctgctgttga ggttcgactg
    58201 gataaatggc tatgggctgc ccgtttttat aaaacccgcg cgctggcccg tgaaatgatt
    58261 gaaggcggta aggtgcatta caacgggcag cgcagcaagc cgagcaaaat cgtcgagctg
    58321 aatgccacgc tcactctgcg ccagggaaat gacgaacgca cggtgattgt aaaggcgatt
    58381 actgaacagc gtcgccccgc cagcgaggca gccttgctgt atgaagagac tgcggaaagt
    58441 gtagagaaac gcgaaaaaat ggcgatggca cgtaaactta atgccttaac catgccgcac
    58501 ccggaccgac gcccggacaa aaaagagcgc cgcgacctgt tacgatttaa acacggcgac
    58561 agtgaataac tgtcacctgc aagagagatg attatgccgc aacatgacca attacatcgc
    58621 tatctgtttg aaaattttgc cgttcgcggc gaactggtaa ccgtttcgga aaccctgcaa
    58681 cagatccttg agaaccacga ttatccgcag cccgttaaaa acgtgctggc agaactgctg
    58741 gttgcgacca gcctgttaac cgctacgctg aagtttgatg gtgatatcac cgtacagctg
    58801 cagggcgacg gtccgatgaa tctggcggtt attaacggta acaataacca gcaaatgcgc
    58861 ggtgtggcgc gcgtgcaggg cgaaattcca gaaaatgccg acctgaaaac gctggtcggc
    58921 aatggttacg tggtgatcac cattaccccg agcgaaggcg aacgctatca gggcgtagtt
    58981 ggtctggaag gtgataccct ggcggcctgc ctagaagatt actttatgcg ttctgaacag
    59041 ctgccgacgc gcctgtttat tcgcaccggc gacgtagacg gcaaaccggc tgcaggcggt
    59101 atgttgttgc aggtaatgcc tgcgcaaaat acccaacagg acgactttga ccacctggcg
    59161 acgctaaccg aaaccatcaa aaccgaagaa ctgctgacct taccggcaaa cgaagtgttg
    59221 tggcgtttgt atcacgaaga agaggtgacg gtttacgatc cgcaggatgt ggagttcaaa
    59281 tgcacctgct cgcgtgaacg ttgcgccgat gcgctgaaaa cgctgcctga tgaagaagtt
    59341 gatagcatcc tggcggaaga tggcgaaatt gacatgcatt gtgattactg cggtaaccac
    59401 tatctgttca atgcgatgga tattgctgaa atccgcaaca acgcgtctcc ggcagatccg
    59461 taagttcatt aatgagtatg tccggcagag agggctctgc cggattttac gacttaatgg
    59521 cctgatgtgc tgcgcttacc agcgcctatg aaataaatcg caaattattt tggtcagata
    59581 cagcgtcgta tccgataaaa gcgttgtcac ttacttaagt ttatttccag cgtttcacca
    59641 tctaccgtca gatcgcgcca cgctttcggc ctcgtcccca gttttccatc cttcgcagct
    59701 gtgagtaatt tttctttcgt tatttcgttc ggaagtaaac tcatatcaag cgcgtcccat
    59761 tcttttctgt ctgagtcaaa gccatagaca atgactctgt catcattaaa agcaaacagg
    59821 atccgctccg cctgaccatc actattcaga tcttgctcga ccagtacgca ggcgtctttt
    59881 tcaatacagg tcatcacgtt atagcgatcc tggattaagg ccgaccagaa tgtcgcatcc
    59941 ggtttaacag aaccaggggc aattaacaca ttttcggcga atactttttc cgatacctgt
    60001 tcctgaagat gttgctctcc atctaacgcc atcagcagat cccgcgcgcg tttcgggtct
    60061 ttcataaacc cggcatcgct tttcagcgac tcaagcgccg cacgtccata gcgaccgctc
    60121 tgttcgagca tgtagatagt tacctggtct ggcgtgtttt tgccgctctg ataacgcgcc
    60181 atatgactgt tcacgctaat acgcatactg tccagcaccg gcgaattaag aagcaccagt
    60241 atcaccaaca ccaataacga aaccgcaagg ttcactttgc cctgaagaac aaggggattt
    60301 tgcccattac gccagacgat actgacaaaa taccccagcg accacaccag taacaccagc
    60361 tccgccagcg cgccctgcaa gcggtcgaca gtccagccgt actgagcgac ccgcagccat
    60421 aacccccagg tggcgacgaa cacatacagc ggagcaacca gcaaagcggt tttaatcagg
    60481 caacgcaacg gccccgtcca gggaagtgac gctttttgcg gatcgcggac aatagccatt
    60541 aagatcaatt gcaaaaaggc cagcgttaac agcaacccgg cggcggagat gtggcgagaa
    60601 atcgcgctca ggcccgtaaa cggcagggtg atgataaaca tcagggttag caatgatact
    60661 aacggcagca accccgtggc gattagcgtg aacaactttt gaatagagtc gattaaacgt
    60721 gactgtgtac gcgcgaggat caccgccagt gcggtaacca gaccaaatgt aagatacata
    60781 aaccagtcgg ttgcaaaaaa gagcgtattg aaaaacgtga tgccaacaag tttaaacaac
    60841 tcaccccaca gtaaaagcac cagccacgtc aggccattgg agagaaaaat caccagtaat
    60901 ataagtacgt tatgccatac tgactggtaa aagtagctat agcggctact gccattgcgg
    60961 atacgaaggc tttgttgtat ccacggtagc aacaacattg ccatcaacag cagatagcag
    61021 ccaaaatccc aaatcgcctt ttcagctctc caggggttca tgccatcagt ttgccacttc
    61081 agccaaccgc tcatccctgt cgtggcaata aacaccagcg ccagccatcc ccaaaggcgc
    61141 ttctgtttaa aagagatcac ggagaagagt aaaacggatg aaaacgcgac ggtcgcaggc
    61201 acgccataaa caatccaact gtgatttttt ccggcaagcc aggcgataag caggtagcaa
    61261 accagcccct gaagtaatcc ggtagcaatc atcccctgaa gtaatccggt agcaatcatc
    61321 ccccagcgtg ttgccggtga aagttcaacg ttatccatgt tggttatcca gaatcaaaag
    61381 gtgggttaat tatcgcatcc gggcagtagt attttgcttt tttcagaaaa taatcaaaaa
    61441 aagttagcgt ggtgaatcga tactttaccg gttgaatttg catcaatttc attcaggaat
    61501 gcgattccac tcacaatatt cccgccatat aaaccaagat ttaacctttt gagaacattt
    61561 tccacaccta aaatgctatt tctgcgataa tagcaaccgt ttcgtgacag gaatcacgga
    61621 gttttttgtc aaatatgaat ttctccagat acgtaaatct atgagccttg tcgcggttaa
    61681 cacccccaaa aagactttac tattcaggca atacatattg gctaaggagc agtgaaatgc
    61741 gcgttaacaa tggtttgccc ccgcaagaac tcgaggctta tggtatcagt gacgtacatg
    61801 atatcgttta caacccaagc tacgacctgc tgtatcagga agagctcgat ccgagcctga
    61861 caggttatga gcgcggggtg ttaactaatc tgggtgccgt tgccgtcgat accgggatct
    61921 tcaccggtcg ttcaccaaaa gataagtata tcgtccgtga cgataccact cgcgatactt
    61981 tctggtgggc agacaaaggc aaaggtaaga acgacaacaa acctctctct ccggaaacct
    62041 ggcagcatct gaaaggcctg gtgaccaggc agctttccgg caaacgtctg ttcgttgtcg
    62101 acgctttctg tggtgcgaac ccggatactc gtctttccgt ccgtttcatc accgaagtgg
    62161 cctggcaggc gcattttgtc aaaaacatgt ttattcgccc gagcgatgaa gagctggcag
    62221 gtttcaaacc agactttatc gttatgaacg gcgcgaagtg cactaacccg cagtggaaag
    62281 aacagggtct gaactccgaa aacttcgtgg cgtttaacct gaccgagcgc atgcagctga
    62341 ttggcggcac ctggtacggc ggcgaaatga agaaagggat gttctcgatg atgaactacc
    62401 tgctgccgct gaaaggtatc gcttctatgc actgctccgc caacgttggc gagaaaggcg
    62461 atgttgcggt gttcttcggc ctttccggca ccggtaaaac caccctttcc accgacccga
    62521 aacgccgcct gattggcgat gacgaacacg gctgggacga tgacggcgtg tttaatttcg
    62581 aaggcggctg ctacgcaaaa actatcaagc tgtcgaaaga agcggaacct gaaatctaca
    62641 acgctatccg tcgtgatgcg ctgctggaaa acgtcaccgt gcgtgaagat ggcactatcg
    62701 actttgatga tggttcaaaa accgagaaca cccgcgtttc ttatccaatc tatcacatcg
    62761 ataacatcgt taagccggtt tccaaagcgg gccacgcgac taaggttatc ttcctgactg
    62821 ctgatgcttt cggcgtgttg ccgccggttt ctcgcctgac tgccgatcaa acccagtatc
    62881 acttcctctc tggcttcacc gccaaactgg ccggtactga gcgtggcatc accgaaccga
    62941 cgccaacctt ctccgcttgc ttcggcgcgg cgttcctgtc gctgcacccg actcagtacg
    63001 cagaagtgct ggtgaaacgt atgcaggcgg cgggcgcgca ggcttatctg gttaacactg
    63061 gctggaacgg cactggcaaa cgtatctcga ttaaagatac ccgcgccatt atcgacgcca
    63121 tcctcaacgg ttcgctggat aatgcagaaa ccttcactct gccgatgttt aacctggcga
    63181 tcccaaccga actgccgggc gtagacacga agattctcga tccgcgtaac acctacgctt
    63241 ctccggaaca gtggcaggaa aaagccgaaa ccctggcgaa actgtttatc gacaacttcg
    63301 ataaatacac cgacacccct gcgggtgccg cgctggtagc ggctggtccg aaactgtaat
    63361 gatttgaagc tggagaatat ctatccagta tcttatagaa agcaaaacgg gaggcacctt
    63421 cgcctcccgt ttatttaccc ttcttttgtc gtgccctgcg cccgcgttac cggcactggc
    63481 agccaggcgc gaatggaaag cccgccccgc tcgctggtgc caagctccag catcccgtta
    63541 tggttatcca cgatacgctg cacaattgcc agccctaatc ccgtgccgct aatggtgcgc
    63601 gcactgtcgc cgcggacaaa cggctggaac aggtgcttac gttgttccgg cgcaattccc
    63661 ggaccgtcat cttccacctg gaaccaggcg cgattcggct ccgttccgct gctgactttg
    63721 atccagccat tgccataacg ggcggcgttg accaccatat tcgccaccgc gcgtttgatc
    63781 gacagcgggt gcattttcac ttcaatgctg ccggggtaaa gcgcggtttc aatttcccgc
    63841 tcatagccac tttcggcagc aatcacctca ccgagcactg cattaagatc cgccatttcc
    63901 atcggcatct cctgcccggt gcgcaggtag tcgataaact gctcaatgat ggcgttgcac
    63961 tcttcgatat ctttattgat cgattctgcc agatagccat cctgctcgct catcatctca
    64021 gtcgccaggc gaatacgcgt cagcggcgtg cgcaagtcgt gacttacccc cgccatcagc
    64081 agcgtgcggt catccgccag ttgcttaaca ccagccgcca tatggttaaa ggcacgggta
    64141 acggaacgca cctccgaagc gccatattca cgcagcggcg gcggaataat ccctttacca
    64201 acctgcaagg ctgcgtgttc gagatcgacc aacggtcggt tctggatacg aataaacagc
    64261 cacgccccgc ctatcgccaa tagcataatc gccagcgtat agcggaacag cggagagaaa
    64321 tcgccctgat gaatttcggt cagcggcacg cgtacccaga tattgggcga cagccaggtt
    64381 ttcagccaga cgacaggcga acttttgttg acctcaacgc gcacttccgt cgggccgccc
    64441 agttgctgtg ccatctgatg gcttaagaat tcatagtgtt gcgcccaacg cagacctgcc
    64501 tcttcggcag cctcgttgga gtagagagag atccccagct cacggtagat ctcccgacgg
    64561 aaagcgggag gcacaaccaa ctgcgtgccg tcctccagtt gcagtttgtc ggtcatcaac
    64621 atacgcactt cgtacgcgag gactttatta aactgctgga ggctcggcaa aatcgcgaag
    64681 ttcagcacca ccagataagt cgtcaccagg ctggcgaaca gcaaggtgac gatgagcaat
    64741 aacgtacggg caaatgaact tcgtggcgag aagcgcaatc gcctcatgct ttagagccgt
    64801 ccggtacaaa gacgtagccc aggccccaaa cggtctgaat gtaacgcgga tgcgctggat
    64861 cttcttctac catgcggcgc agacgcgaaa tctgcacgtc gatggagcgt tccattgcgg
    64921 aatattcacg accacgggca aggttcatca gcttatcgcg ggagagcggc tcacgcggat
    64981 ggctgaccag tgccttcagt accgcaaact caccgctggt gagcggcatc ggctcatctt
    65041 cgcggaacat ttcgcgcgta ccgaggttaa gtttgaactt accgaaagca attaccgcct
    65101 cttcctgtga cggtgcgcct ggcagttcgt tcgcctgacg acgcagcacc gcacgaatac
    65161 gggccagcag ttcacgcggg ttaaacggtt ttggaatgta gtcgtcagcg ccaatctcca
    65221 ggcctacgat acggtccact tcttcccctt tcgccgtcac cataatgatc ggcatcgggt
    65281 tgctctgact acgaagacgt cggcaaatcg acaagccatc ttcaccaggt aacattaaat
    65341 ccagtaccat aagatggaaa gattcacgag tcagcaggcg atccatctgt tctgcattag
    65401 cgacgcttcg aacctggaag ccttgttcgg tgagataacg ttccagcagc gcacgcaggc
    65461 gcatgtcgtc atcgaccacc agaatcttgt agttctcttg cattgtttgt actcccaaag
    65521 gttcgcaaca atttgtaagc gtgtattctt aaaaaagctc acgttcgtca ccagctaaat
    65581 ctggtatgaa tttcagccta aattgttaca aagcatatta aacagcagct taagtataca
    65641 atttattcgg cgaaacatta ttgattctgt tgatatgatc acgttatacc caatgtgcgc
    65701 attatcaaac agacaaaggg aatcaacgag atgaaaacgc ccctggttac ccgggaaggg
    65761 tatgaaaaac tcaaacaaga gcttaattat ctctggcgtg aagaacgccc ggaggtcaca
    65821 aaaaaggtga cctgggccgc aagtctgggc gaccgcagcg aaaatgctga ctatcagtat
    65881 aataaaaagc gtctgcgtga aatcgaccgt cgcgtgcgct atctcactaa atgcctggaa
    65941 aatctcaaaa tcgtcgatta ctcccctcag caggaaggca aagtcttttt tggcgcgtgg
    66001 gtggagattg aaaacgacga tggcgtgact caccgtttcc gtattgtcgg ctacgatgaa
    66061 atttttggcc gtaaagatta catctctatc gattccccga tggcccgcgc attgctgaaa
    66121 aaagaagtcg gcgatctggc ggtggtgaat acccctgccg gggaagcgaa ctggtatgtt
    66181 aatgctatcg agtacgtgaa accgtaagga agagtcttaa cctcctgccg atggctggca
    66241 tttttgccag ccagtccgta taactatccc ctgatttttg atccgaaaag atgaactcaa
    66301 accatgatga atgattcgtt ctgccgcatt attgcgggtg aaattcaggc gcgcccggaa
    66361 caggttgacg ctgccgttcg cctgcttgac gaagggaata ccgtgccgtt tatcgcacgt
    66421 tatcgtaagg aaatcaccgg cggtctggat gacacgcagc tgcgtaatct ggaaacgcgt
    66481 ctgagctatc tgcgcgagct ggaagagaga cgtcaggcga tcctcaaatc catttccgag
    66541 caaggcaaac tcaccgatga tctggcgaag gccatcaacg ccaccctaag caaaaccgaa
    66601 ctcgaagacc tctacctgcc ctacaaacct aaacgccgca cccgcgggca aatcgccatt
    66661 gaagcagggc ttgagccgtt ggctgacctg ctgtggagcg atccgtcaca cacgccagaa
    66721 gtcgccgctg cacaatatgt tgatgccgat aaaggcgtgg cagataccaa agccgcgctg
    66781 gatggcgcgc gctatatcct gatggaacgg tttgccgaag atgccacgct gctggcgaaa
    66841 gtgcgtgatt atctgtggaa gaacgcgcat ttggtttcta cggtggtgag cggtaaagaa
    66901 gaggaagggg cgaaattccg cgactatttc gatcatcacg aaccgttgtc cacggtgcct
    66961 tctcaccgcg cgctggcgat gttccgtggg cgtaacgaag gcgtactcca gctttcgctg
    67021 aatgccgatc cgcagttcga tgagccgccc aaagagagct attgcgagca aatcatcatg
    67081 gatcaccttg gcctgcgcct gaacaatgcc ccggcggata gctggcgcaa aagcgtagtg
    67141 agctggacct ggcgcatcaa ggtgctgatg catctggaaa ccgaactgat gggtaccgtg
    67201 cgcgaacgcg cggaagatga agcaatcaac gtctttgccc gtaacctgca cgatctgctg
    67261 atggcggccc ctgccggact gcgtgcaacg atgggcctcg atccgggtct gcgtaccggg
    67321 gtaaaagtgg cggtggtcga tgccactggc aaactggtgg cgaccgatac catttacccg
    67381 cacaccggac aagccgcaaa agcagcgatg accgttgctg ccttgtgtga aaaacataac
    67441 gttgaactgg tggcgatcgg caacggtaca gcttcccgcg aaactgaacg tttctatctc
    67501 gacgtgcaga agcagttccc gaaagtgacc gcacagaaag tgatcgtcag cgaagctggc
    67561 gcgtcggttt actcagcttc cgagctggca gcgcaggagt tcccggatct cgacgtttcg
    67621 ctgcgtggtg cggtatctat cgcccgccgt ttgcaggatc cgctggcgga gctggtgaaa
    67681 atcgatccga aatctatcgg cgtaggtcag tatcagcatg acgtcagcca gacgcaactg
    67741 gcccgcaaac tggatgcagt agtagaagac tgcgtaaacg ccgttggcgt cgatctcaac
    67801 accgcttctg ttccgctatt aacccgcgtg gcgggcctga cgcgcatgat ggcgcaaaac
    67861 atcgtggcct ggcgcgatga gaacggccag ttccagaacc gtcagcaact gctgaaagtc
    67921 agccgtctgg ggccgaaagc cttcgagcag tgcgcgggct tcctgcgcat taaccacggt
    67981 gataacccgc tggacgcctc taccgttcat ccggaagcct atccggtggt ggaacgcatt
    68041 ctggcagcaa cacagcaggc actgaaagat ctgatgggta acagcagcga actgcgtaac
    68101 ctgaaagcgt ctgactttac tgatgaaaaa ttcggtgtgc cgacagtaac tgacatcatc
    68161 aaagagctgg aaaaaccggg tcgcgatccg cgtccggaat ttaaaaccgc tcagtttgcc
    68221 gatggcgtcg agacaatgaa cgacctgcaa ccgggtatga tcctcgaagg cgcagtgacc
    68281 aacgtcacca actttggcgc gtttgtcgat attggcgtgc atcaggacgg cctggttcac
    68341 atctcttcat tgtcgaacaa gtttgtggaa gatccgcata ccgtggtgaa agcgggcgac
    68401 attgtgaagg tgaaagtgct ggaagtggat cttcagcgta aacgtatcgc cctgactatg
    68461 cgtctggacg agcagcctgg cgaaaccaac tctcgtcgcg gcggcggtaa tgaacgcccg
    68521 caaaacaacc gcccggcagc caaaccacgc ggtcgtgaag cgcagcctgc cggtaatagc
    68581 gcgatgatgg atgcgctggc ggcagcgatg ggtaaaaaac gttaaacgcc cggactggcc
    68641 cctaaacctg cgcgataagg cgttcacgcc gcatctggca acgaacgctt tgcctgacat
    68701 aaaagtgccg gagaatatct ccggcacttt tattccacag ccaaactcac aatatactcc
    68761 gttaatattt atcacttcat taacaactga aaccttaatt aaacattagc cagtccgggt
    68821 aactcactat tcgaattata ttttcgctgc gatataacct tgagccacat caacattgag
    68881 tcagattatt attcaaacca acattcgcac acattttaag tattgctgat agaaaccatt
    68941 ctcattatca ttgtgttgtt gattatttaa tctctccttt gttggcaaat catctggtct
    69001 catgtcgcgg tcaaacgccc catgaggtag ttatccagtt aatgagaaac aagtaggcac
    69061 ctatgcaata cactccagat actgcgtgga aaatcactgg cttttcccgt gaaatcagcc
    69121 cggcatatcg ccaaaaactg ctttctctag gcatgttacc tggctcctct tttgatgtgg
    69181 tgcgcgtcgc tccactcggc gaccccattc atatcgaaac ccgtcgtgtg agcctggtat
    69241 tacgcaaaaa agatctggcc ttattagaag tggaagcggt ttcctgttaa tacggtgata
    69301 acaacaatga aaaaattaac cattggctta attggtaatc caaattctgg caagacaacc
    69361 ttatttaacc agctcactgg cgcacgtcag cgtgtaggta actgggctgg cgttaccgtc
    69421 gaacgtaaag aagggcaatt ctccaccacc gatcatcagg tcacgctggt ggacctgccc
    69481 ggcacctatt ctctgaccac catctcatcg cagacctcgc tcgatgagca aatcgcctgt
    69541 cactacattt tgagtggcga cgccgacctg ctgattaacg tggtggatgc gtctaacctt
    69601 gagcgtaacc tgtacctgac gctgcaactg ctggaactcg gcattccctg catcgtggcg
    69661 ctgaacatgc tcgacattgc cgagaagcaa aatattcgta ttgaaattga tgctctatcg
    69721 gcacgtcttg gctgcccggt gatcccgctg gtttcaaccc gtggtcgcgg tattgaagcg
    69781 ctcaagctgg cgattgatcg ctataaagct aacgagaatg tggaactggt gcattacgca
    69841 cagccgctac tcaacgaagc agattcactg gcaaaagtga tgccttccga catcccgctg
    69901 aaacaacgtc gctggctggg cctgcaaatg ctggaaggcg atatctacag ccgcgcctac
    69961 gccggtgaag cgtcgcagca tctggatgcc accctcgccc gtctgcgtaa tgaaatggat
    70021 gatccggcgc tgcacattgc cgatgcgcgt taccagtgca ttgctgccat ctgtgatgtg
    70081 gtaagcaaca ccctgacggc agaacccagc cgtttcacca ctgcggtaga taaaatcgtg
    70141 ctcaaccgtt tcctcggtct gccgattttc ctctttgtga tgtacctgat gttcctgctg
    70201 gctatcaaca tcggcggggc gttacagccg ctgtttaacg tcggctccgt ggcgctattt
    70261 gtgcatggta ttcaatggat tggctacacg ctccacttcc cggactggct gactatcttc
    70321 ctcgcccagg gcctgggtgg cggtattaac accgtgctgc cactggtgcc gcagatcggc
    70381 atgatgtacc tgttcctctc cttccttgag gactccggct atatggcgcg tgcagcgttt
    70441 gtgatggacc gcctgatgca ggcgctgggc ttgccgggga aatcctttgt gccgctgatc
    70501 gtcggtttcg gttgtaacgt accgtcggta atgggcgcac gtacgcttga tgcaccgcgt
    70561 gaacgtctga tgaccatcat gatggcaccg tttatgtcct gcggcgcgcg tctggctatc
    70621 ttcgcagtat tcgcggctgc cttcttcggg cagaacggtg ccctggcggt tttctcgctg
    70681 tatatgctcg gtatcgtgat ggcggtgctg actggcctga tgctcaagta caccatcatg
    70741 cgcggtgaag cgacgccgtt tgtcatggag ctgccggtct accatgtacc acacgttaaa
    70801 agcctgatta tccagacctg gcagcgtctg aagggcttcg ttctgcgtgc tggtaaagtg
    70861 attatcatcg tcagcatttt cctgagcgct ttcaacagct tctcgttgag cgggaaaatc
    70921 gtcgataaca tcaacgactc ggcgctggct tccgtcagcc gggtgataac ccctgtcttc
    70981 aagccgattg gcgtacatga agataactgg caggcaacgg ttggcctgtt tacgggcgca
    71041 atggcaaaag aagtggtggt gggtacgctc aacaccctct acaccgcaga aaatattcag
    71101 gacgaagagt tcaatccggc ggaattcaac ctcggtgaag agctgttcag tgcggtagat
    71161 gaaacctggc agagcctgaa agacaccttc agccttagcg tactgatgaa ccccattgaa
    71221 gctagcaaag gcaacggtga aatgggtacc ggggcgatgg gcgtgatgga tcagaaattc
    71281 ggtagcgcag ctgccgcata cagctacctg attttcgtcc tgctgtatgt accatgtatc
    71341 tcggtgatgg gagccatcgc ccgtgaatca agccgtggct ggatgggctt ctccatcctg
    71401 tgggggctga atatcgctta ctcactggca acattgttct atcaggtcgc cagctacagt
    71461 cagcacccaa cttacagcct ggtgtgcatt ctggcggtaa tcctgtttaa catcgtagct
    71521 atcggtctgc tgcgccgcgc gcgtagccgg gtggatatcg aactgctggc aacccgcaag
    71581 tcggtaagca gttgctgcgc agccagcacc accggtgatt gccattaatg gcttcactta
    71641 ttcaggtacg tgatttgctg gcgttacggg gccgtatgga agcgacccag ataagccaga
    71701 cattgaacac tccgcagcca atgattaacg ccatgctgca acagctggaa agcatgggca
    71761 aagccgtgcg gattcaggaa gaacctgacg gctgcctctc tggcagttgt aaaagctgcc
    71821 cggaaggcaa agcctgtctg cgcgagtggt gggcgctgcg ttaaccatac tccatcgcct
    71881 gttttgaaaa gcccggtatg cgtttgcatc cgggcttttt tgcgtgcggc tttccataaa
    71941 aatgcaattc ttgcagcgca acgtaagttc ctctgaaagc gtctcgcagg gatgaaaact
    72001 cgctaatgca cagatgtgga gtggcgcgta gagtcgcggc attcaaacaa caggtgaagg
    72061 aacgccatga gtaaaaagca gagttccacc ccacatgatg cgctgttcaa actcttttta
    72121 cgccaaccgg agacggcacg cgattttctt gcgtttcatt taccggcacc cattcacgcg
    72181 ctctgtgata tgaaaaccct caagctggag tcgaggagct ttattgatga cgatctgcgt
    72241 gaaagctatt ccgatgtgct gtggtcggtg aaaacggaac aaggaccagg atacatctat
    72301 tgtctgattg aacatcaaag cacctcaaac aaactgatcg catttcgcat gatgcgttac
    72361 gctattgccg caatgcaaaa tcaccttgat gctggataca aaacgttgcc gatggtggtg
    72421 ccattgttgt tttaccacgg tattgaaagc ccctatccct attcgctgtg ttggctggat
    72481 tgtttcgccg atcccaaact ggcaaggcag ctttatgcct ccgcatttcc gctgattgat
    72541 gtcaccgtca tgcctgatga tgaaatcatg cagcaccgac gtatggcgct gctggagtta
    72601 attcaaaaac atattcgtca acgcgatctg atggggctgg tagagcaaat ggcctgctta
    72661 ttaagtagtg gatacgctaa tgacagacaa atcaaagggc tgtttaatta catactgcaa
    72721 actggcgacg ctgtacgttt taacgatttt atcgacggcg ttgccgaacg ttcaccgaaa
    72781 cacaaggaga gtttaatgac tattgcggaa agattgcggc aggaggggga acaatccaaa
    72841 gccctgcata tagccaaaat aatgcttgaa tccggagtcc ctcttgcaga catcatgcga
    72901 tttaccgggc tgtcagaaga agagttggct gcgg
//
LOCUS       DAJTRC010000005        71887 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.005, whole genome shotgun sequence.
ACCESSION   DAJTRC010000005 DAJTRC010000000
VERSION     DAJTRC010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 71887)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71887)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71887
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.005"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(1134..2720)
                     /locus_tag="OO357_000374"
     CDS             complement(1134..2720)
                     /locus_tag="OO357_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate permease LctP family transporter"
                     /protein_id="HCR5885440.1"
                     /translation="MVTWTQMYMPMGGLGLSALVALIPIIFFFVALAVLRLKGHVAGA
                     ITLILSILIAIFAFKMPIDMAFAAAGYGFIYGLWPIAWIIVAAVFLYKLTVASGQFDI
                     IRSSVISITDDQRLQVLLIGFSFGALLEGAAGFGATVAFGALGVPILVAGQVTGIDPF
                     HIGAMAGRQLPFLSVLVPFWLVAMMDGWKGVKETWPAALVAGGSFAVTQFFTSNYIGP
                     ELPDITSALVSIVSLALFLKVWRPKNTETAISMGQSAGAMVVNKPSSGGPVPSEYSLG
                     QIIRAWSPFLILTVLVTIWTMKPFKALFAPGGAFYSLVINFQIPHLHQQVLKAAPIVA
                     QPTPMDAVFKFDPLSAGGTAIFIAAIISIFILGVGIKKGIGVFAEMLISLKWPILSIG
                     MVLAFAFVTNYSGMSTTLALVLAGTGVMFPFFSPFLGWLGVFLTGSDTSSNALFGSLQ
                     STTAQQINVSDTLLVAANTSGGVTGKMISPQSIAVACAATGMVGRESELFRYTVKHSL
                     IFASVITLLQAYVFTGMLVS"
     gene            complement(3075..5246)
                     /gene="glcB"
                     /locus_tag="OO357_000375"
     CDS             complement(3075..5246)
                     /gene="glcB"
                     /locus_tag="OO357_000375"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase G"
                     /protein_id="HCR5885441.1"
                     /translation="MSQTITQGRLRIDANFKRFVDGEVLPGVELDAAAFWHNVDEIVH
                     DLAPENRQLLAERDRIQAALDEWHRSNPGPVKDKAAYKSFLRELGYLVPQPERVTVET
                     TGIDSEITSQAGPQLVVPAMNARYALNAANARWGSLYDALYGSDIIPQEGAMVSGYDP
                     QRGEQVIAWVRRFLDESLPLENGSYQDVVAFKVVDKQLRIQLKNGKETTLRTPAQFVG
                     YRGDAAAPTCILLKNNGLHIELQIDANGRIGKDDPAHINDVIVEAAISTILDCEDSVA
                     AVDAEDKILLYRNLLGLMQGTLQEKMEKNGRQIVRKLNDDRQYTAADGSEISLHGRSL
                     LFIRNVGHLMTIPVIWDSEGNEIPEGILDGVMTGAIALYDLKVQKNSRTGSVYIVKPK
                     MHGPQEVAFANKLFTRIETMLGMAPNTLKMGIMDEERRTSLNLRSCIAQARNRVAFIN
                     TGFLDRTGDEMHSVMEAGPMLRKNQMKSTPWIKAYERNNVLSGLFCGLRGKAQIGKGM
                     WAMPDLMADMYSQKGDQLRAGANTAWVPSPTAATLHALHYHHTNVQSVQANIAQTEFS
                     AEFEPLLDDLLTIPVAENANWSAQEIQQELDNNVQGILGYVVRWVEQGIGCSKVPDIH
                     NVALMEDRATLRISSQHIANWLRHGILTKEQVQASLENMAKVVDQQNAGDPAYRPMAG
                     NFANSCAFKAASDLIFLGVKQPNGYTEPLLHAWRLREKESH"
     gene            complement(5268..5672)
                     /locus_tag="OO357_000376"
     CDS             complement(5268..5672)
                     /locus_tag="OO357_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding protein"
                     /protein_id="HCR5885442.1"
                     /translation="MKTKVILSQQMASAIIAAGQEEAQKNNWSVSIAVADDGGHLLAL
                     SRMDDCAPIAAYISQEKARTAALGRRATKGYEEMVNNGRTAFVTAPLLTSLEGGVPVV
                     VDGQIIGAVGVSGLTGAQDAQVAKVAAAVLAK"
     gene            complement(5677..6896)
                     /gene="glcF"
                     /locus_tag="OO357_000377"
                     /pseudo
     CDS             complement(5677..6896)
                     /gene="glcF"
                     /locus_tag="OO357_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026190.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
     gene            complement(6907..7959)
                     /gene="glcE"
                     /locus_tag="OO357_000378"
     CDS             complement(6907..7959)
                     /gene="glcE"
                     /locus_tag="OO357_000378"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HCR5885443.1"
                     /translation="MLRECDYSQALLEQVNQAISDKTPLVIQGSNSKAFLGRPVTGQT
                     LDVRCHRGIVNYDPTELVITARAGTPLVAIEAALESAGQMLPCEPPHYGEEATWGGMV
                     ACGLAGPRRPWSGSVRDFVLGTRIITGTGKHLRFGGEVMKNVAGYDLSRLMAGSYGCL
                     GVLTEISMKVLPRPRASLSLRREISLQEAMSEIAEWQLQPLPISGLCYFDNALWIRLE
                     GGEGSVKAARELLGGEEVVGQFWQQLREQQLPFFSLPGTLWRISLPSDAPMMDLPGEQ
                     LIDWGGALRWLKSTADDNQIHRIARNAGGHATRFSAGDGGFAPLSAPLFRYHQQLKQQ
                     LDPCGVFNPGRMYAEL"
     gene            complement(7959..9458)
                     /gene="glcD"
                     /locus_tag="OO357_000379"
                     /pseudo
     CDS             complement(7959..9458)
                     /gene="glcD"
                     /locus_tag="OO357_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417453.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcD"
     gene            9709..10479
                     /gene="glcC"
                     /locus_tag="OO357_000380"
     CDS             9709..10479
                     /gene="glcC"
                     /locus_tag="OO357_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708791.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GlcC"
                     /protein_id="HCR5885444.1"
                     /translation="MKDERRPICEVVAESIERLIIDGVLKVGQPLPSERRLCEKLGFS
                     RSALREGLTVLRGRGIIETAQGRDSHVARLNREQDTSPLIHLFSTQPRTLYDLLDVRA
                     LLEGESARLAATLGTQADFVVITRCYEKMLAASENHKEISLIEHAQLDHAFHLAICQA
                     SHNQVLVFTLQSLTDLMFNSVFASVNNLYHRPQQKKQIDRQHARIYNAVLQRLPHVAQ
                     RAARDHVRTVKKNLHDIELEGHHLIRSAVPLEMNKVGM"
     gene            complement(10603..11744)
                     /locus_tag="OO357_000381"
                     /pseudo
     CDS             complement(10603..11744)
                     /locus_tag="OO357_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001327865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
     gene            12106..13836
                     /locus_tag="OO357_000382"
     CDS             12106..13836
                     /locus_tag="OO357_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000262881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acyl-AMP ligase"
                     /protein_id="HCR5885445.1"
                     /translation="MVYMSNKIFTHSLPMRYADFPTLVDALDYAALSRAGMNFYDRRC
                     QLEDQLEYQTLKTRAEAGAKRLLSLNLKKGERVALIAETSSGFVEAFFACQYAGLVAV
                     PLAIPMGVGQRDSWSAKLQGLLASCQPAAIITGDEWLPLVNAATHNNPELHVLSHAWF
                     KALPEADVALQRPVPNDIAYLQYTSGSTRFPRGVIITHREVMANLRAISHDGIKLRPS
                     DRCVSWQPFYHDMGLVGFLLPPVATQLSVDYLRTQDFAMRPLQWLKLISKNRGTVSVA
                     PPFGYELCQRRVNEKDLAELDLSCWRVAGIGAEPISAEQLHQFAECFRQVNFDNKTFM
                     PCYGLAENALAVSFSDEASGVVVNEVDRDILEYQGKAVAPGAETRAVSTFVNCGKALP
                     EHGIEIRNEAGMPVAERVVGHICISGPSLMSGYFGDQVSQDEIAATGWLDTGDLGYLL
                     DGYLYVTGRIKDLIIIRGRNIWPQDIEYIAEQEPEIHSGDAIAFVTAQEKIILQIQCR
                     ISDEERRGQLIHALAARIQSEFGVTAAIDLLPPHSIPRTSSGKPARAEAKKRYQKAYA
                     ASLNVQESLA"
     gene            13833..14747
                     /locus_tag="OO357_000383"
     CDS             13833..14747
                     /locus_tag="OO357_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001481799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HCR5885446.1"
                     /translation="MNQTVAVTGATGFIGKYIIDNLLARGFHVRALTRTARAHVNDNL
                     TWVRGSLEDTHSLSELVAGASVVVHCAGQVRGHKEEIFTRCNVDGSLRLMQAAKESGF
                     CQRFLFISSLAARHPELSWYAKSKYVAEQRLAAMADEITLGVFRPTAVYGPGDKELKP
                     LFDWMLRGLLPRLGAPDTQLSFLHVTDFAQAVGQWLSAETVQTQTYELCDGVAGGYDW
                     QRVRQLAAKARCGSVRMVDIPLPVLTGLADISTALSRLAGKEPMLTRSKIRELTHADW
                     SASNNRISEDINWFPGISLEHALRNGLF"
     gene            14780..15028
                     /locus_tag="OO357_000384"
     CDS             14780..15028
                     /locus_tag="OO357_000384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5885447.1"
                     /translation="MVNREIVMDYILSCLQDLVENGVEIKPDSDLVNDLGLESIKVMD
                     LLMMLEDRFDISIPINILLDVKTPAQLMETLLPWLENK"
     gene            15028..16199
                     /locus_tag="OO357_000385"
                     /pseudo
     CDS             15028..16199
                     /locus_tag="OO357_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
     gene            complement(16235..17314)
                     /gene="lptG"
                     /locus_tag="OO357_000386"
     CDS             complement(16235..17314)
                     /gene="lptG"
                     /locus_tag="OO357_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5885448.1"
                     /translation="MNVFSRYLIRHLFLGFAAAAGLLLPLFTTFNLINELDGVSPGGY
                     RWTQAVLVVLMTLPRTLVELSPFIALLGGIVGLGQLSKNSELTAIRSTGFSIFRIALV
                     ALVAGILWTVSLGAIDEWVASPLQQQALQIKSTATALGEDDDITGNMLWARRGNEFVT
                     VKSLNEQGQPVGVEIFHYRDDLSLESYIYARSATIEDDKTWVLHGVNHKKWLNGKETL
                     ETLDNLAWQSAFTSMNLEELSMPGNTFSVRQLNHYIHYLQETGQPSSEYHLALWEKLG
                     QPILTLAMILLAVPFTFSAPRSPGMGSRLAVGVIVGLLTWISYQIMVNLGLLFALSAP
                     VTALGLPVAFVLVALSLVYWYDRQH"
     gene            complement(17311..18381)
                     /gene="lptF"
                     /locus_tag="OO357_000387"
     CDS             complement(17311..18381)
                     /gene="lptF"
                     /locus_tag="OO357_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5885449.1"
                     /translation="MKLVEHYIMRGTRRLVLIIVGFLIFIFASYSAQRYLTEAANGTL
                     ALDVVLDIVFYKVLIALEMLLPVGLYVSVGVTLGQMYTDSEITAISAAGGSPGRLYKA
                     VLYLAIPLSIFVTLLSMYGRPWAYAQIYQLEQQSQSELDVRQLRAKKFNTNDNGRMIL
                     SQTVDQDNNRLTDALIYTSTANRTRIFRAGSVDVVDPSPEKPTVMLHNGTAYLLDHQG
                     RDDNEQIYRNLQLHLNPLDQSPNVKRKAKSVTELARSAFPADHAELQWRQSRGLTALL
                     MALLAISLSRVKPRQGRFSTLLPLTLLFIAIFYGGDVCRTLVANGAIPLIPGLWLVPG
                     LMLMGLLILVARDFSLLQKFSR"
     gene            complement(18412..18972)
                     /locus_tag="OO357_000388"
     CDS             complement(18412..18972)
                     /locus_tag="OO357_000388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5885450.1"
                     /translation="MSSRLIIALIIMLLAPGVQAHNFVTGKTVTPVYIQEGGELLLNS
                     DDEIHYQKWNSTQLAGKVRIIQYIAGRKSAKKKNSLLIKAVEAANFPQDRFQPTTIVN
                     TDDAIFGTGYFVVGKIEKNKRRYPWAQFVIDGNGQGRVAWRLPEQSSTILVLNKAGQI
                     QWAKDGSLTPEEVDHVIALAQKLINE"
     gene            complement(18984..19808)
                     /locus_tag="OO357_000389"
     CDS             complement(18984..19808)
                     /locus_tag="OO357_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885451.1"
                     /translation="MMRKYFPLEASERLFVAIEEDDVVDAQVSLPPTIALSCTTEIIH
                     DNYALCLQFWLNGVDRQELLRLVRKQAKGDELTADERKQFKYMRARYKHLRFAQRLYL
                     KKHQAGFLFGKTTVFLGRFQDGFRNGKKNIVSYYGNLLRIYLSSPVWSLVNYSLRHSQ
                     LESVSSFIAYRQKQMHTLKEIIAKPRLTGREFHDVRKIISQQVSYYDTLRSLDPENKE
                     ALQISRFLAAINGLMGDKHDDMVADDMENRQSYYAPLALDSDICQRLELLISRFPL"
     gene            complement(19808..21157)
                     /locus_tag="OO357_000390"
     CDS             complement(19808..21157)
                     /locus_tag="OO357_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HCR5885452.1"
                     /translation="MAGFNIKHWFADGAFRTIIRNSAWLGSSNVESALLGLLALSCAG
                     KGMTPAMFGVLVIVQSYAKSISDFIKFQTWQLVVQYGTPALTNNNPQQFRNVVSFSFS
                     LDIVSGAVAIVGGIALLPFLSHSLGLDDQSFWLAALYCTLIPSMASSTPTGILRAVDR
                     FDLIAVQQATKPFLRAAGSVAAWYFDFGFVGFVIAWYVSNLVGGTMYWWFAARELRRR
                     NIHNAFKLNLFESARHIKGAWSFVWSTNIAHSIWSARNSCSTVLVGIVLGPAAAGLFK
                     IAMTFFDAAGTPAGLLGKSFYPEVMRLDPRTTRPWLLGVKSGLLAGGIGILVALAVLI
                     VGKPLISLVFGVKYLEAYDLIQVMLGAIVISMLGFPQESLLLMAGKQRAFLVAQTIAS
                     IGYIVLLFMFCHLFGVLGAAFAYFGGQCLDVALSLIPTLKAFFQRHSLLYNAAGEKS"
     gene            complement(21203..21961)
                     /locus_tag="OO357_000391"
     CDS             complement(21203..21961)
                     /locus_tag="OO357_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885453.1"
                     /translation="MDALQTQTVNSTTAPQPNYIPGLIAVVGCDGTGKSTLTTDLVKS
                     LQQHWQTERRYLGLLSGEDGDKIKRLPLVGVWLERRLAAKSSKTQSMKTKSPALWAAV
                     IMYCFSLRRMANLRKVQRLAQSGVLVVSDRFPQAEISGFYYDGPGIGVERATGKISMF
                     LAQRERSLYQKMAQYRPELIIRLGIDIDTAISRKPDHEYAELQDKIGVMSTIGYNGTK
                     ILEIDSRAPYSEVLEQVQKAVSLVAIVSDRRSLT"
     gene            complement(21993..22706)
                     /locus_tag="OO357_000392"
     CDS             complement(21993..22706)
                     /locus_tag="OO357_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5885454.1"
                     /translation="MNQRNMSIINSTPVRVIAIVGCDGSGKSTLTASLVNELAARMPT
                     EHIYLGQSSGRIGEWISQLPVIGAPCGCYLRSKAAHVHEKPLTPPGNITALVIYLLSC
                     WRAYKFRKMLCKSQQGYLLITDRYPQVEVPGFRFDGPQLAKTTGGNGWIKMLRQRELK
                     LYQWMASYLPVLLIRLGIDEQTAFARKPDHQLAALQEKIAVTPQLTFNGAKILELDGR
                     QPADEIMQASLRAIHAALS"
     gene            22880..23572
                     /locus_tag="OO357_000393"
     CDS             22880..23572
                     /locus_tag="OO357_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate kinase"
                     /protein_id="HCR5885455.1"
                     /translation="MQSITPPLIAVIGSDASGKSTVCEHLITVVEKYGAAERVHLGKQ
                     AGNVGRAVTKLPLMGKSLHKTIERNQVKTAKKLPGPVPALVITAFVARRLLRFRHMLA
                     CRRRGLIVLTDRYPQDQIPGAYDGTVFPPNVEGGRFVSWLASQERKAFHWMASHKPDL
                     VIKLNVDLEVACARKPDHKRESLARKIAITPQLTFGGAQLVDIDANQPLEQVLVDAEK
                     AITDFMTARGYH"
     gene            complement(23621..25120)
                     /gene="pitB"
                     /locus_tag="OO357_000394"
     CDS             complement(23621..25120)
                     /gene="pitB"
                     /locus_tag="OO357_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter PitB"
                     /protein_id="HCR5885456.1"
                     /translation="MLNLFVGLDIYTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMQPQLAVVMAAFFNFFGVLLGGLSVAYAIVHMLPIDLLLNMGSTHGLAMVFSMLL
                     AAIIWNLGTWFFGLPASSSHTLIGAIIGIGLTNALLTGSSVMDALNLREVTKIFSSLI
                     VSPIVGLVIAGGLIFLLRRFWSGTKKRDRIHRIPEDRKKKKGKRKPPFWTRIALIVSA
                     AGVAFSHGANDGQKGIGLVMLVLVGIAPAGFVVNMNASGYEITRTRDAVTNFEHYLQQ
                     HPELPQKLIAMEPPLPAASTDGTQVTEFHCHPANTFDAIARVKTMLPGNMESYEPLSV
                     SQRSQLRRIMLCISDTSAKLAKLPGVSKEDQNLLKKLRSDMLSTIEYAPVWIIMAVAL
                     ALGIGTMIGWRRVAMTIGEKIGKRGMTYAQGMAAQMTAAVSIGLASYIGMPVSTTHVL
                     SSAVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAIFLSGGLYWIALQLI"
     gene            complement(25412..27271)
                     /gene="gss"
                     /locus_tag="OO357_000395"
     CDS             complement(25412..27271)
                     /gene="gss"
                     /locus_tag="OO357_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutathionylspermidine
                     amidase/synthase"
                     /protein_id="HCR5885457.1"
                     /translation="MSKGTTSQDAPFGTLLGYAPGGVAIYSSDYSSLDPQEYEDDAVF
                     RSYIDDEYMGHKWQCVEFARRFLFLNYGVVFTDVGMAWEIFSLRFLREVVNDNILPLQ
                     AFPNGSPRAPVAGALLIWDKGGEFKDTGHVAIITQLHGNKVRIAEQNVIHSPLPQGQQ
                     WTRELEMVVENGCYTLKDTFDDTTILGWMIQTEDTEYSLPQPEIAGELLKISGARLEN
                     KGQFDGKWLDEKDPLQNAYVQANGQVINQDPYHYYTITESAEQELIKATNELHLMYLH
                     ATDKVLKDDNLLALFDIPKILWPRLRLSWQRRRHHMITGRMDFCMDERGLKVYEYNAD
                     SASCHTEAGLILERWAEQGYKGNGFNPAEGLINELAGAWKHSRARPFVHIMQDKDIEE
                     NYHAQFMEQALHQAGFETRILRGLDELGWDAAGQLIDGEGRLVNCVWKTWAWETAFDQ
                     IREVSDREFAAVPIRTGHPQNEVRLIDVLLRPEVLVFEPLWTVIPGNKAILPILWSLF
                     PHHRYLLDTDFTVNDELVKTGYAVKPIAGRCGSNIDLVSHHEEVLDKTSGKFAEQKNI
                     YQQLWCLPKVDGKYIQVCTFTVGGNYGGTCLRGDESLVIKKESDIEPLIVVKK"
     gene            27476..28342
                     /gene="yghU"
                     /locus_tag="OO357_000396"
     CDS             27476..28342
                     /gene="yghU"
                     /locus_tag="OO357_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HCR5885458.1"
                     /translation="MTDNTYQPAKVWTWDKSAGGAFANINRPFSGPTHEKTLPVGKHP
                     LQLYSLGTPNGQKVTIMLEELLALGVTGAEYDAWLIRIGDGDQFSSGFVEVNPNSKIP
                     ALRDHTHNPPIRVFESGSILLYLAEKFGYFLPQDLAKRTETLNWLFWLQGAAPFLGGG
                     FGHFYHYAPVKIEYAINRFTMEAKRLLDVLDKQLAQHKFVAGDEYTIADMAIWPWFGN
                     VVLGGVYDAAEFLDAGSYKHVQRWAKEVGERPAVKRGRIVNRTNGPLNEQLHERHDAS
                     DFETNTEDKRQG"
     gene            complement(28492..28740)
                     /gene="hybG"
                     /locus_tag="OO357_000397"
     CDS             complement(28492..28740)
                     /gene="hybG"
                     /locus_tag="OO357_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation factor HybG"
                     /protein_id="HCR5885459.1"
                     /translation="MCIGVPGQVLAVGEDIHQLAQVEVCGIKRDVNIALICEGNPADL
                     LGQWVLVHVGFAMSIIDEDEAKATLDALRQMDYDITSA"
     gene            complement(28874..29215)
                     /gene="hypA"
                     /locus_tag="OO357_000398"
     CDS             complement(28874..29215)
                     /gene="hypA"
                     /locus_tag="OO357_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5885460.1"
                     /translation="MHELSLCQSAVEIIQRQAEQHDVKRVTAVWLEIGALSCVEESAV
                     RFSFEIVCHGTVAQGCDLHIVYKPAQAWCWDCSQVVEIHQHDAQCPLCHGERLRVDTG
                     DSLIVKSIEVE"
     gene            complement(29208..29696)
                     /gene="hybE"
                     /locus_tag="OO357_000399"
     CDS             complement(29208..29696)
                     /gene="hybE"
                     /locus_tag="OO357_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-2 assembly chaperone"
                     /protein_id="HCR5885461.1"
                     /translation="MTEEIAGFQTSPKAQVQAAFEEIARRSMHDLSFLHPSMPVYVSD
                     FTLFEGQWTGCVITPWMLSAVIFPGPDQLWPLRKVSEKIGLQLPYGTMTFTVGELDGV
                     SQYLSCSLMSPLSHSMSIEEGQRLTDDCARMILSLPVTNPDVPHAGRRALLFGRRRGE
                     NA"
     gene            complement(29689..30183)
                     /locus_tag="OO357_000400"
     CDS             complement(29689..30183)
                     /locus_tag="OO357_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HyaD/HybD family hydrogenase maturation
                     endopeptidase"
                     /protein_id="HCR5885462.1"
                     /translation="MRILVLGVGNILLTDEAIGVRIVEALEQRYILPDYVEILDGGTA
                     GMELLGDMANRDHLIIADAIVSKKNTPGTMMILRDEEVPALFTNKISPHQLGLADVLS
                     ALRFTGEFPKKLTLVGVIPESLEPHIGLTPTVEAMIEPALEQVLAALRESGVEAIPRE
                     AIHD"
     gene            complement(30183..31886)
                     /gene="hybC"
                     /locus_tag="OO357_000401"
     CDS             complement(30183..31886)
                     /gene="hybC"
                     /locus_tag="OO357_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 large subunit"
                     /protein_id="HCR5885463.1"
                     /translation="MSQRITIDPVTRIEGHLRIDCEIENGVVSKAWASGTMWRGMEEI
                     VKNRDPRDAWMIVQRICGVCTTTHALSSVRAAESALNIDVPVNAQYIRNIILAAHTTH
                     DHIVHFYQLSALDWVDITSALQADPTKASEMLKGVSTWHLNSPEEFTKVQNKIKDLVA
                     SGQLGIFANGYWGHPAMKLPPEVNLIAVAHYLQALECQRDANRVVALLGGKTPHIQNL
                     AVGGVANPINLDGLGVLNLERLMYIKSFIDKLSDFVEQVYKVDTAVIAAFYPEWLTRG
                     KGAVNYLSVPEFPTDSKNGSFLFPGGYIENADLSSYRPITSHSDEYLIKGIQESAKHS
                     WYKDEAPQAPWEGTTIPAYDGWSDDGKYSWVKSPTFYGKTVEVGPLANMLVKLAAGRE
                     STQNKLNEIVAIYQKLTGNTLEVAQLHSTLGRIIGRTVHCCELQDILQNQYSALITNI
                     GKGDHTTFVKPNIPATGEFKGVGFLEAPRGMLSHWMVIKDGIISNYQAVVPSTWNSGP
                     RNFNDDVGPYEQSLVGTPVADPNKPLEVVRTIHSFDPCMACAVHVVDADGNEVVSVKV
                     L"
     gene            complement(31883..33061)
                     /gene="hybB"
                     /locus_tag="OO357_000402"
     CDS             complement(31883..33061)
                     /gene="hybB"
                     /locus_tag="OO357_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase cytochrome b subunit"
                     /protein_id="HCR5885464.1"
                     /translation="MSHDPQPLGGKIISKPVMIFGPLIVICMLLIVKRLVFGLGSVSD
                     LNGGFPWGVWIAFDLLIGTGFACGGWALAWAVYVFNRGQYHPLVRPALLASLFGYSLG
                     GLSITIDVGRYWNLPYFYIPGHFNVNSVLFETAVCMTIYIGVMALEFAPALFERLGWK
                     VSLQRLNKVMFFIIALGALLPTMHQSSMGSLMISAGYKVHPLWQSYEMLPLFSLLTAF
                     IMGFSIVIFEGSLVQAGLRGNGPDEKSLFVKLTNTISVLLAIFIGLRFGELIYRDKLS
                     LAFAGDFYSVMFWIEVLLMLFPLVVLRVAKLRNDSRMLFLSALSALLGCATWRLTYSL
                     VAFNPGGGYAYFPTWEELLISIGFVAIEICAYIVLIRLLPILPPLKQNDHNRHEASKA
                     "
     gene            complement(33051..34037)
                     /gene="hybA"
                     /locus_tag="OO357_000403"
     CDS             complement(33051..34037)
                     /gene="hybA"
                     /locus_tag="OO357_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 operon protein HybA"
                     /protein_id="HCR5885465.1"
                     /translation="MNRRNFIKAASCGALLTGALPSVSHAAAENRPPIPGSLGMLYDS
                     TLCVGCQACVTKCQDINFPERNPQGEQTWSNNDKLSPYTNNIIQVWTSGTGVNKDQEE
                     NGYAYIKKQCMHCVDPNCVSVCPVSALKKDPKTGIVHYDKDVCTGCRYCMVACPYNVP
                     KYDYNNPFGALHKCELCNQKGVERLDKGGLPGCVEVCPAGAVIFGTREELMAEAKKRL
                     ALKPGSEYHYPRQTLKSGDTYLHTVPKYYPHLYGEKEGGGTQVLVLTGVPYENLDLPK
                     LDDLSTGARSENIQHTLYKGMMLPLAVLAGLTVLVRRNTKNDHHDGGDDHES"
     gene            complement(34040..35158)
                     /gene="hybO"
                     /locus_tag="OO357_000404"
     CDS             complement(34040..35158)
                     /gene="hybO"
                     /locus_tag="OO357_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 2 small subunit"
                     /protein_id="HCR5885466.1"
                     /translation="MTGDNTLIHSHGINRRDFMKLCAALAATMGLSSKAAAEMAESVT
                     NPQRPPVIWIGAQECTGCTESLLRATHPTVENLVLETISLEYHEVLSAAFGHQVEENK
                     HNALEKYKGQYVLVVDGSIPLKDNGIYCMVAGEPIVDHIRKAAEGSAAIIAIGSCSAW
                     GGVAAAGVNPTGAVSLQEVLPGKTVINIPGCPPNPHNFLATVAHIITYGKPPKLDDKN
                     RPTFAYGRLIHEHCERRPHFDAGRFAKEFGDEGHREGWCLYHLGCKGPETYGNCSTLQ
                     FCDVGGVWPVAIGHPCYGCNEKGIGFHKGIHQLANVENQTPRSQKPDVNAKEGGNVSA
                     GAIGLLGGVVGLVAGVSVMAVRELGRQQKKDNADSRGE"
     gene            complement(35347..35634)
                     /locus_tag="OO357_000405"
     CDS             complement(35347..35634)
                     /locus_tag="OO357_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2623 domain-containing protein"
                     /protein_id="HCR5885467.1"
                     /translation="MNNHFGKGLMAGLKATHADSAVNVTKFCADYKRGFVLGYSHRMY
                     EKTGDRQLSAWEAGILTRRYGLDKEMVMDFFRENNSCSTLRFFMAGYRLEN"
     gene            complement(35753..36640)
                     /locus_tag="OO357_000406"
     CDS             complement(35753..36640)
                     /locus_tag="OO357_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311911.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5885468.1"
                     /translation="MPRLTAKDFPQELLDYYDYYVHGKISKREFLNLAAKYAVGGMTA
                     LALFDLLKPNYALATQVEFTDPEIVAEYITYPSPNGHGEVRGYLVKPAKMSGKTPAVG
                     VVHENRGLNPYIEDVARRVAKAGYIALAPDGLSSVGGYPGNDDKGRELQQQVDPTKLM
                     NDFFAAIEFMQRYPQATGKVGITGFCYGGGVSNAAAVAYPELACAVPFYGRQAPTADV
                     AKIEAPLLLHFAELDTRINEGWPPYEAALKDNNKVYEAYIYPGVNHGFHNDSTPRYDK
                     SAADLAWQRTLKWFDKYLS"
     gene            36797..37837
                     /gene="gpr"
                     /locus_tag="OO357_000407"
     CDS             36797..37837
                     /gene="gpr"
                     /locus_tag="OO357_000407"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-glyceraldehyde 3-phosphate reductase"
                     /protein_id="HCR5885469.1"
                     /translation="MVWLANPERYGQMQYRYCGKSGLRLPALSLGLWHNFGHVNALES
                     QRAILRKAFDLGITHFDLANNYGPPPGSAEENFGRLLREDFAAYRDELIISTKAGYDM
                     WPGPYGSGGSRKYLLASLDQSLKRMGLEYVDIFYSHRVDENTPMEETASALAHAVQSG
                     KALYVGISSYSPERTQKMVELLHEWKIPLLIHQPSYNLLNRWVDKSGLLDTLQNNGVG
                     CIAFTPLAQGLLTGKYLNGIPQDSRMHREGNKVRGLTPKMLTEANLNSLRLLNEMAQQ
                     RGQSMAQMALSWLLKDDRVTSVLIGASRAEQLEENVQALNNLTFSTEELAQIDQHIAD
                     GELNLWQASSDK"
     gene            complement(37877..38371)
                     /locus_tag="OO357_000408"
     CDS             complement(37877..38371)
                     /locus_tag="OO357_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708819.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HCR5885470.1"
                     /translation="MERFLENAMYASRWLLAPVYFGLSLALVALALKFFQEIIHVLPN
                     IFSMAESDLILVLLSLVDMTLVGGLLVMVMFSGYENFVSQLDISENKEKLKWLGKMDA
                     TSLKNKVAASIVAISSIHLLRVFMDAKNVPDNKLMWYVIIHLTFVLSAFVMGYLDRLT
                     RHNH"
     gene            38562..39446
                     /gene="yghA"
                     /locus_tag="OO357_000409"
     CDS             38562..39446
                     /gene="yghA"
                     /locus_tag="OO357_000409"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5885471.1"
                     /translation="MSHLKDPTTQYYTGEYPKQKQPTPGIQAKMTPIPDCGEKTYVGS
                     GRLKDRKALVTGGDSGIGRAAAIAYAREGADVAISYLPVEEEDAQDVKKIIEECGRKA
                     VLLPGDLSDEKFARSLVHEAHKALGGLDIMALVAGKQVAIPDIADLTSEQFQKTFAIN
                     VFALFWLTQEAIPLLPKGASVITTSSIQAYQPSPHLLDYAATKAAILNYSRGLAKQVA
                     EKGIRVNIVAPGPIWTALQISGGQTQDKIPQFGQKTPMKRAGQPAELAPVYVYLASQE
                     SSYVTAEVHGVCGGEHLG"
     gene            complement(39718..40143)
                     /gene="exbD"
                     /locus_tag="OO357_000410"
     CDS             complement(39718..40143)
                     /gene="exbD"
                     /locus_tag="OO357_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein ExbD"
                     /protein_id="HCR5885472.1"
                     /translation="MAMHLNENLDDNGEMHDINVTPFIDVMLVLLIIFMVAAPLATVD
                     VKVNLPASTSTPQPRPEKPVYLSVKADNSMFIGNDPVTDETMITALNALTEGKKDTTI
                     FFRADKTVDYETLMKVMDTLHQAGYLKIGLVGEETAKAK"
     gene            complement(40150..40884)
                     /gene="exbB"
                     /locus_tag="OO357_000411"
     CDS             complement(40150..40884)
                     /gene="exbB"
                     /locus_tag="OO357_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5885473.1"
                     /translation="MGNNLMQTDLSVWGMYQYADIVVKCVMIGLILASVVTWAIFFSK
                     SVEFFNQKRRLKREQQLLAEARSLNQANDIAADFGSKSLSLHLLNEAQNELELSEGSD
                     DNEGIKERTSFRLERRVAAVGRQMGRGNGYLATIGAISPFVGLFGTVWGIMNSFIGIA
                     QTQTTNLAVVAPGIAEALLATAIGLVAAIPAVVIYNVFARQIGGFKAMLGDVAAQVLL
                     LQSRDLDLEASAAAHPVRVAQKLRAG"
     gene            41136..42323
                     /gene="metC"
                     /locus_tag="OO357_000412"
     CDS             41136..42323
                     /gene="metC"
                     /locus_tag="OO357_000412"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine beta-lyase"
                     /protein_id="HCR5885474.1"
                     /translation="MADKKLDTQLVNAGRSKKYTLGAVNSVIQRASSLVFESMEAKKH
                     ATRNRANGELFYGRRGTLTHFSLQQAMCELEGGAGCALFPCGAAAVANSILAFVEQGD
                     HVLMTNTAYEPSQDFCSKILSKLGVTTSWFDPLIGADIVKHLQPNTKIVFLESPGSIT
                     MEVHDVPAIVAAVRSVVPDAIIMIDNTWAAGVLFKALDFGIDVSIQAATKYLVGHSDA
                     MIGTAVCNARCWEQLRENAYLMGQMVDADTAYITSRGLRTLGVRLRQHHESSLKVAEW
                     LAEHPQVARVNHPALPGSKGHEFWKRDFTGSSGLFSFVLKKKLNDEELANYLDNFSLF
                     SMAYSWGGYESLILANQPEHIAAIRPQGEIDFSGTLIRLHIGLEDVDDLIADLDAGFA
                     RIV"
     gene            42463..43122
                     /locus_tag="OO357_000413"
     CDS             42463..43122
                     /locus_tag="OO357_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5885475.1"
                     /translation="MAVIQDIIAALWQHDFAALADPHIVSVVYFVMFATLFLENGLLP
                     ASFLPGDSLLILAGALIAQGVMDFLPTIAILTAAASLGCWLSYIQGSWLGNTKTVKGW
                     LAQLPAKYHQRATCMFDRHGLLALLAGRFLAFVRTLLPTMAGISGLPNRRFQFFNWLS
                     GLLWVSVVTSFGYALSMIPFVKRHEDQVMTFLMILPIALLTAGLLGTLFVVIKKKYCN
                     A"
     gene            complement(43162..44061)
                     /gene="yqhC"
                     /locus_tag="OO357_000414"
     CDS             complement(43162..44061)
                     /gene="yqhC"
                     /locus_tag="OO357_000414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001407451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YqhC"
                     /protein_id="HCR5885476.1"
                     /translation="MKREEICRLLADKVNKLKNKENSLSELLPDVRLLYGETPFARTP
                     VMYEPGIIILFSGHKIGYINERVFRYDANEYLLLTVPLPFECETYATSEVPLAGLRLN
                     VDILQLQELLMDIGEDEHFQPSMAASGINSATLSEEILCAAERLLDVMERPLDARILG
                     KQIIREILYYVLTGPCGGALLALVSRQTHFSLISRVLKRIENKYTENLSVEQLAAEAN
                     MSVSAFHHNFKSVTSTSPLQYLKNYRLHKARMMIIHDGMKASAAAMRVGYESASQFSR
                     EFKRYFGVTPGEDAARMRAMQGN"
     gene            44255..45418
                     /gene="yqhD"
                     /locus_tag="OO357_000415"
     CDS             44255..45418
                     /gene="yqhD"
                     /locus_tag="OO357_000415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HCR5885477.1"
                     /translation="MNNFNLHTPTRILFGKGAIAGLREQIPHDARVLITYGGGSVKKT
                     GVLDQVLDALKGMDVLEFGGIEPNPAYETLMNAVKLVREQKVTFLLAVGGGSVLDGTK
                     FIAAAANYPENIDPWHILQTGGKEIKSAIPMGCVLTLPATGSESNAGAVISRKTTGDK
                     QAFHSAHVQPVFAVLDPVYTYTLPPRQVANGVVDAFVHTVEQYVTKPVDAKIQDRFAE
                     GILLTLIEDGPKALKEPENYDVRANVMWAATQALNGLIGTGVPQDWATHMLGHELTAM
                     HGLDHAQTLAIVLPALWNEKRETKRAKLLQYAERVWNITEGSDDERIDAAIAATRNFF
                     EQLGVPTHLSDYGLDGSSIPALLKKLEEHGMTQLGENHDITLDVSRRIYEAAR"
     gene            45523..46350
                     /gene="dkgA"
                     /locus_tag="OO357_000416"
     CDS             45523..46350
                     /gene="dkgA"
                     /locus_tag="OO357_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417485.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgA"
                     /protein_id="HCR5885478.1"
                     /translation="MANPTVIKLQDGNVMPQLGLGVWQASNEEVITAIQKALEVGYRS
                     IDTAAAYKNEEGVGKALKNASVNREELFITTKLWNDDHKRPREALLDSLKKLQLDYID
                     LYLMHWPVPAIDHYVEAWKGMIELQKEGLIKSIGVCNFQIHHLQRLIDETGVTPVINQ
                     IELHPLMQQRQLHAWNATHKIQTESWSPLAQGGKGVFDQKVIRDLADKYGKTPAQIVI
                     RWHLDSGLVVIPKSVTPSRIAENFDVWDFRLDKDELGKIAKLDQGKRLGPDPDQFGG"
     gene            46550..47476
                     /locus_tag="OO357_000417"
     CDS             46550..47476
                     /locus_tag="OO357_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708829.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjP/YqhG family protein"
                     /protein_id="HCR5885479.1"
                     /translation="MKIILLFLAALASFTVHAQPPSQTVEQTVRQIYQNYKSDASTPY
                     FGETGERAITSARIQQALTLNDNLTLPGNIGWLDYDPVCDCQDFGDLVLESVAITQTD
                     VDHADAVVRFRIFKDDKEKTTQTLKMVAENGRWVIDDIVSNHGSVLQAVNSENEKTLA
                     ALASLQKEQPESFVAELFEHIADYSWPWTWVVSDSYRQAVNAFYKTTFKTANNPDEDM
                     QIERQFIYDNPICFGEESLFSRVDEIRVLEKTADSARIHVRFTLTNGNNEEQELVLQR
                     REGKWEIADFIRPNSGSLLKQIEAKTAARLKQ"
     gene            47527..47784
                     /gene="yqhH"
                     /locus_tag="OO357_000418"
     CDS             47527..47784
                     /gene="yqhH"
                     /locus_tag="OO357_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YqhH"
                     /protein_id="HCR5885480.1"
                     /translation="MKTIFTVGAVVLATCLLSGCVNEQKVNQLASNVQTLNAKIARLE
                     QDMKALRPQIYAAKSEANRANTRLDAQDYFDCLRCLRMYAE"
     gene            complement(47827..50046)
                     /locus_tag="OO357_000419"
     CDS             complement(47827..50046)
                     /locus_tag="OO357_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708831.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HCR5885481.1"
                     /translation="MSSISLIQPDRDLFSWPQYWAACFGPAPFLPMSREEMDQLGWDS
                     CDIILVTGDAYVDHPSFGMAICGRMLEAQGFRVGIIAQPDWSSKDDFMRLGKPNLFFG
                     VTAGNMDSMINRYTADRRLRHDDAYTPDNVAGKRPDRATLVYTQRCKEAWKDVPVILG
                     GIEASLRRTAHYDYWSDTVRRSVLVDSKADMLMFGNGERPLVEVAHRLAMGEPISEIR
                     DVRNTAIIVKEALPGWSGVDSTRLDTLGKIDPIPHPYGEDLPCADNKPVAPKKQEAKA
                     VIVQPPRQKPWEKTYVLLPSFEKVKGDKVLYAHASRILHHETNPGCARALMQKHGDRY
                     VWINPPAIPLSTEEMDSVFALPYKRVPHPAYGNARIPAYEMIRFSVNIMRGCFGGCSF
                     CSITEHEGRIIQSRSEDSIINEIEAIRDTVPGFTGVISDLGGPTANMYMLRCKSPRAE
                     QTCRRLSCVYPDICPHMDTNHEPTINLYRRARDLKGIKKILIASGVRYDIAVEDPRYI
                     KELATHHVGGYLKIAPEHTEEGPLSKMMKPGMGSYDRFKELFDTYSKQAGKEQYLIPY
                     FISAHPGTRDEDMVNLALWLKKHRFRLDQVQNFYPSPLANSTTMYYTGKKPLAKIGYK
                     SEGVFVPKGDKQRRLHKALLRYHDPANWPLIRQALEAMGKKHLIGSRRDCLVPAPTIE
                     EMREARRQNRNTRPALTKHTPMATQRQTPATAKKASSTQSRPVNAGAKKRPKAAVGR"
     gene            complement(50157..51569)
                     /gene="ftsP"
                     /locus_tag="OO357_000420"
     CDS             complement(50157..51569)
                     /gene="ftsP"
                     /locus_tag="OO357_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsP"
                     /protein_id="HCR5885482.1"
                     /translation="MSLSRRQFIQASGIALCAGAVPLKASAAGQQQPLPVPPLLESRR
                     GQPLFMTVQRAHWSFTPGTRASVWGINGRYLGPTIRVWKGDDVKLIYSNRLTENVSMT
                     VAGLQVPGPLMGGPARMMSPNADWAPVLPIRQNAATLWYHANTPNRTAQQVYNGLAGM
                     WLVEDEVSKSLPIPNHYGVDDFPVIIQDKRLDNFGTPEYNEPGSGGFVGDTLLVNGVQ
                     SPYVEVSRGWVRLRLLNASNSRRYQLQMSDGRPLHVISGDQGFLPAPVSVKQLSLAPG
                     ERREILVDMSNGDEVSITCGEAASIVDRIRGFFEPSSILVSTLVLTLRPTGLLPLVTD
                     SLPMRLLPTEIMAGSPIRSRDISLGDDPGINGQLWDVNRIDVTAQQGTWERWTVRADE
                     PQAFHIEGVMFQIRNVNGAMPFPEDRGWKDTVWVDGQVELLVYFGQPSWAHFPFYFNS
                     QTLEMADRGSIGQLLVNPVP"
     gene            complement(51644..52381)
                     /gene="plsC"
                     /locus_tag="OO357_000421"
                     /gene_synonym="parF"
     CDS             complement(51644..52381)
                     /gene="plsC"
                     /locus_tag="OO357_000421"
                     /gene_synonym="parF"
                     /EC_number="2.3.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acylglycerol-3-phosphate O-acyltransferase"
                     /protein_id="HCR5885483.1"
                     /translation="MLYIFRLIITVIYSILVCVFGSIYCLFSPRNPKHVATFGHMFGR
                     LAPLFGLKVECRKPADAESYGNAIYIANHQNNYDMVTASNIVQPPTVTVGKKSLLWIP
                     FFGQLYWLTGNLLIDRNNRTKAHGTIAEVVNHFKKRRISIWMFPEGTRSRGRGLLPFK
                     TGAFHAAIAAGVPIIPVCVSTTSNKINLNRLHNGLVIVEMLPPIDVSQYGKDQVRELA
                     AHCRSIMEQKIAELDKEVAEREAAGKV"
     gene            complement(52615..54873)
                     /gene="parC"
                     /locus_tag="OO357_000422"
     CDS             complement(52615..54873)
                     /gene="parC"
                     /locus_tag="OO357_000422"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HCR5885484.1"
                     /translation="MSDMAERLALHEFTENAYLNYSMYVIMDRALPFIGDGLKPVQRR
                     IVYAMSELGLNASAKFKKSARTVGDVLGKYHPHGDIACYEAMVLMAQPFSYRYPLVDG
                     QGNWGAPDDPKSFAAMRYTESRLSKYSELLLSELGQGTADWVPNFDGTLQEPKMLPAR
                     LPNILLNGTTGIAVGMATDIPPHNLREVAQAAIALIDQPKTTLDQLLDIVQGPDYPTE
                     AEIITSRAEIRKIYENGRGSVRMRAVWKKEDGAVVISALPHQVSGARVLEQIAAQMRN
                     KKLPMVDDLRDESDHENPTRLVIVPRSNRVDMDQVMNHLFATTDLEKSYRINLNMIGL
                     DGRPAVKNLLEILSEWLVFRRDTVRRRLNYRLEKVLKRLHILEGLLVAFLNIDEVIEI
                     IRNEDEPKPALMSRFGLTETQAEAILELKLRHLAKLEEMKIRGEQSELEKERDQLQGI
                     LASERKMNNLLKKELQADAQAYGDDRRSPLQEREEAKAMSEHDMLPSEPVTIVLSQMG
                     WVRSAKGHDIDAPGLNYKAGDSFKAAVKGKSNQPVVFVDSTGRSYAIDPITLPSARGQ
                     GEPLTGKLTLPPGATVDHMLMESDDQKLLMASDAGYGFVCTFNDLVARNRAGKALITL
                     PENAHVMPPVVIEDASDMLLAITQAGRMLMFPVSDLPQLSKGKGNKIINIPSAEAARG
                     EDGLAQLYVLPPQSTLTIHVGKRKIKLRPEELQKVTGERGRRGTLMRGLQRIDRVEID
                     SPRRASSGDSEE"
     gene            complement(55419..55901)
                     /locus_tag="OO357_000423"
     CDS             complement(55419..55901)
                     /locus_tag="OO357_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HCR5885485.1"
                     /translation="MTNLTLDVNIIDFPSIPVAMLPHRCSPELLNYSVAKFIMWRKET
                     GLSPVNQSQTFGVAWDAPATTAPEAFRFDICGSVSEPIPDNRYGVSNGELTGGRYAVA
                     RHVGELDDISHTIWGIIRHWLPASGEKMRKAPILFHYTNLAEGVTEQRLETDVYVPLA
                     "
     gene            complement(55954..56346)
                     /gene="ygiW"
                     /locus_tag="OO357_000424"
     CDS             complement(55954..56346)
                     /gene="ygiW"
                     /locus_tag="OO357_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708836.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OB fold stress tolerance protein YgiW"
                     /protein_id="HCR5885486.1"
                     /translation="MKKFAAVIAVMALCSAPVMAAEQGGFSGPSVTQSQAGGFQGPNG
                     SVTTVESAKSLRDDTWVTLRGNIVERISDDLYVFKDASGTINVDIDHKRWNGVTVTPK
                     DTVEIQGEVDKDWNSVEIDVKQIRKVNP"
     gene            56498..57157
                     /gene="qseB"
                     /locus_tag="OO357_000425"
     CDS             56498..57157
                     /gene="qseB"
                     /locus_tag="OO357_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator QseB"
                     /protein_id="HCR5885487.1"
                     /translation="MRILLIEDDMLIGDGIKTGLSKMGFSVDWFTQGRQGKEALYSAP
                     YDAVILDLTLPGMDGRDILREWREKGQREPVLNLTARDALAERVEGLRLGADDYLCKP
                     FALIEVAARLEALMRRTNGQASNELRHGNVMLDPGKRIATLAGEPLTLKPKEFALLEL
                     LMRNAGRVLPRKLIEEKLYTWDEEVTSNAVEVHVHHLRRKLGSDFIRTVHGIGYTLGE
                     K"
     gene            57154..58428
                     /gene="qseC"
                     /locus_tag="OO357_000426"
     CDS             57154..58428
                     /gene="qseC"
                     /locus_tag="OO357_000426"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     QseC"
                     /protein_id="HCR5885488.1"
                     /translation="MKFTQRLSLRVRLTLIFLILASVTWLLSSFVAWKQTTDNVDELF
                     DTQLMLFAKRLSTLDLNEINAADRMAQTPNKLKHGHVDDDALTFAIFTHDGRMVLNDG
                     DNGEDIPYSYQREGFADGQLVGEDDPWRFVWMTSPDGKYRIVVGQEWEYREDMALAIV
                     AGQLIPWLVALPIMLIIMMVLLGRELAPLNKLALALRMRDPDSEKPLNATGVPSEVRP
                     LVESLNQLFARTHAMMVRERRFTSDAAHELRSPLTALKVQTEVAQLSDDDPQARKKAL
                     LQLHSGIDRATRLVDQLLTLSRLDSLDNLQDVAEIPLEDLLQSSVMDIYHTAQQAKID
                     VRLTLNAHGIKRTGQPLLLSLLVRNLLDNGPGVTPEALARIGERFYRPPGQTATGSGL
                     GLSIVQRIAKLHDMNVEFGNAEQGGFEAKVSW"
     gene            complement(58474..58806)
                     /locus_tag="OO357_000427"
     CDS             complement(58474..58806)
                     /locus_tag="OO357_000427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2645 family protein"
                     /protein_id="HCR5885489.1"
                     /translation="MLKQKIKTIFEALPYIMLTYWLIDSFFAFNKYDWMLESGGNICS
                     IPSVSGEDRILQAMIAAFFLLTPLIILILRKLFMREMFEFWLYVFSLVICLVCGWWLF
                     WGRFIFCY"
     gene            59125..59706
                     /gene="mdaB"
                     /locus_tag="OO357_000428"
     CDS             59125..59706
                     /gene="mdaB"
                     /locus_tag="OO357_000428"
                     /EC_number="1.6.5.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase MdaB"
                     /protein_id="HCR5885490.1"
                     /translation="MSNILIINGAKKFAHSNGQLNDTLTEVADGTLRDLGHDVRIVRA
                     DSDYNVKAEVQNFLWADVVIWQMPGWWMGAPWTVKKYIDDVFTEGHGTLYASDGRTRK
                     DPSKKYGSGSLVQDKKYMLSLTWNAPMEAFTEKDQFFHGVGVDGVYLPFHKANQFLGM
                     EPLPTFIANDVIKMPDVPRYTEEYRKHLVEIFG"
     gene            59737..60051
                     /locus_tag="OO357_000429"
     CDS             59737..60051
                     /locus_tag="OO357_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HCR5885491.1"
                     /translation="MLTVIAEIRTRPGQHHRQAVLDQFAKIVPTVLKEEGCHGYAPMV
                     DCAAGVSFQSMAPDSIVMIEQWESIAHLEAHLQTPHMKAYSEAVKGDVLEMNIRILQP
                     GI"
     gene            complement(60099..61991)
                     /gene="parE"
                     /locus_tag="OO357_000430"
     CDS             complement(60099..61991)
                     /gene="parE"
                     /locus_tag="OO357_000430"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HCR5885492.1"
                     /translation="MTQTYNADAIEVLTGLEPVRRRPGMYTDTTRPNHLGQEVIDNSV
                     DEALAGHAKRVDVILHADQSLEVIDDGRGMPVDIHPEEGVPAVELILCRLHAGGKFSN
                     KNYQFSGGLHGVGISVVNALSKRVEVNVRRDGQVYNIAFENGEKVQDLQVVGTCGKRN
                     TGTSVHFWPDETFFDSPRFSVSRLTHVLKAKAVLCPGVEITFKDEINNTEQRWCYQDG
                     LNDYLAEAVNGLPTLPEKPFIGNFAGDTEAVDWALLWLPEGGELLTESYVNLIPTMQG
                     GTHVNGLRQGLLDAMREFCEYRNILPRGVKLSAEDIWDRCAYVLSVKMQDPQFAGQTK
                     ERLSSRQCAAFVSGVVKDAFILWLNQNVQAAELLAEMAISSAQRRMRAAKKVVRKKLT
                     SGPALPGKLADCTAQGLNRTELFLVEGDSAGGSAKQARDREYQAIMPLKGKILNTWEV
                     SSDEVLASQEVHDISVAIGIDPDSDDLSQLRYGKICILADADSDGLHIATLLCALFVK
                     HFRALVKHGHVYVALPPLYRIDLGKEVYYALTEEEKEGVLEQLKRKKGKPNVQRFKGL
                     GEMNPMQLRETTLDPNTRRLVQLTIDDEDDQRTDAMMDMLLAKKRSEDRRNWLQEKGD
                     MAEIEV"
     gene            complement(62020..62601)
                     /gene="yqiA"
                     /locus_tag="OO357_000431"
     CDS             complement(62020..62601)
                     /gene="yqiA"
                     /locus_tag="OO357_000431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase YqiA"
                     /protein_id="HCR5885493.1"
                     /translation="MSTLLYLHGFNSSPRSAKASLLKNWLAEHHPDVEMIIPQLPPYP
                     SDAAELLESIVLEHGGDSLGIVGSSLGGYYATWLSQCFMLPAVVVNPAVRPFELLTDY
                     LGQNENPYTGQQYVLESRHIYDLKVMQIDPLEAPDLIWLLQQTGDEVLDYRQAVAYYA
                     SCRQTVIEGGNHAFTGFEDYFNPIVDFLGLHHL"
     gene            complement(62601..63428)
                     /gene="cpdA"
                     /locus_tag="OO357_000432"
     CDS             complement(62601..63428)
                     /gene="cpdA"
                     /locus_tag="OO357_000432"
                     /EC_number="3.1.4.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3',5'-cyclic-AMP phosphodiesterase"
                     /protein_id="HCR5885494.1"
                     /translation="MESLLTLPLAGEARGRILQITDTHLFAQKHEALLGVNTWESYQA
                     VLEAIRPHQHEFDLIVATGDLAQDQSSAAYQHFAEGIASFRAPCVWLPGNHDFQPAMY
                     SALQDAGISPAKRVFIGEQWQILLLDSQVFGVPHGELSEFQLEWLERKLADAPERHTL
                     LLLHHHPLPAGCSWLDQHSLRNAGELDTVLAKFPHVKYLLCGHIHQELDLDWNGRRLL
                     ATPSTCVQFKPHCSNFTLDTIAPGWRTLELHADGTLTTEVHRLADTRFQPDTASEGY"
     gene            complement(63453..63875)
                     /locus_tag="OO357_000433"
     CDS             complement(63453..63875)
                     /locus_tag="OO357_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1249 family protein"
                     /protein_id="HCR5885495.1"
                     /translation="MKRYTPDFPEMMRLCEMNFSQLRRLLPRNDAPGETVSYQVANAQ
                     YRLTIVESTRYTTLVTIEQTAPAISYWSLPSMTVRLYHDAMVAEVCSSQQIFRFKARY
                     DYPNKKLHQRDEKHQINQFLADWLRYCLAHGAMAIPVY"
     gene            complement(63876..64505)
                     /gene="nudF"
                     /locus_tag="OO357_000434"
     CDS             complement(63876..64505)
                     /gene="nudF"
                     /locus_tag="OO357_000434"
                     /EC_number="3.6.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribose diphosphatase"
                     /protein_id="HCR5885496.1"
                     /translation="MLKPDNLPVTFGKNDVEIIARETLYRGFFSLDLYRFRHRLFNGQ
                     MSHEVRREIFERGHAAVLLPFDPVRDEVVLIEQIRIAAYDTSETPWLLEMVAGMIEEG
                     ESVEDVARREAIEEAGLIVKRTKPVLSFLASPGGTSERSSIMVGEVDATTASGIHGLA
                     DENEDIRVHVVSREQAYQWVEEGKIDNAASVIALQWLQLHHQALKNEWA"
     gene            64710..66191
                     /gene="tolC"
                     /locus_tag="OO357_000435"
     CDS             64710..66191
                     /gene="tolC"
                     /locus_tag="OO357_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane channel protein TolC"
                     /protein_id="HCR5885497.1"
                     /translation="MKKLLPILIGLSLSGFSSLSQAENLMQVYQQARLSNPELRKSAA
                     DRDAAFEKINEARSPLLPQLGLGADYTYSNGYRDANGINSNATSASLQLTQSIFDMSK
                     WRALTLQEKAAGIQDVTYQTDQQTLILNTATAYFNVLNAIDVLSYTQAQKEAIYRQLD
                     QTTQRFNVGLVAITDVQNARAQYDTVLANEVTARNNLDNAVEQLRQITGNYYPELAAL
                     NVENFKTDKPQPVNALLKEAEKRNLSLLQARLSQDLEREQIRQAQDGHLPTLDLTAST
                     GISDTSYSGSKTRGAAGTQYDDSNMGQNKVGLSFSLPIYQGGMVNSQVKQAQYNFVGA
                     SEQLESAHRSVVQTVRSSFNNINASISSINAYKQAVVSAQSSLDAMEAGYSVGTRTIV
                     DVLDATTTLYNAKQELANARYNYLINQLNIKSALGTLNEQDLLALNNALSKPVSTNPE
                     NVAPQTPEQNAIADGYAPDSPAPVVQQTSARTTTSNGHNPFRN"
     gene            66339..67010
                     /locus_tag="OO357_000436"
     CDS             66339..67010
                     /locus_tag="OO357_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417509.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 family protein"
                     /protein_id="HCR5885498.1"
                     /translation="MKRTKSIRHASFRKNWSARHLTPVALAVATVLMLAGCEKSDETV
                     SLYQNADDCSAANPGKSAECTTAYNNALKEAERTAPKYATREDCVAEFGEGQCQQAPA
                     QAGMAPENQAQAQQSSGSFWMPLMAGYMMGRLMGGGAGFAQQPLFSSKNPASPAYGKY
                     TDATGKNYGAAQPGRTMTVPKTAMAPKPATTTTVTRGGFGESVAKQSTMQRSATGTSS
                     RSMGG"
     gene            67016..68176
                     /locus_tag="OO357_000437"
     CDS             67016..68176
                     /locus_tag="OO357_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5885499.1"
                     /translation="MERVSITERPDWREKAHEYGFNFHTMYGEPYWCEDAYYKLTLAQ
                     VEKLEEVTAELHQMCLKVVEKVIASDELMTKFRIPKHTWSFVRQSWLTHQPSLYSRLD
                     LAWDGTGEPKLLENNADTPTSLYEAAFFQWIWLEDQLNAGNLPEGSDQFNSLQEKLID
                     RFVELREQYGFQLLHLTCCRDTVEDRGTIQYLQDCATEAEIATEFLYIDEIGLGEKGQ
                     FTDLQDQVISNLFKLYPWEFMLREMFSTKLEDAGVRWLEPAWKSIISNKALLPLLWEM
                     FPNHPNLLPAYFAEDDHPQMEKYVVKPIFSREGANVSIIENGKTIEAAEGPYGEEGMI
                     VQQFHPLPKFGDSYMLIGSWLVNDQPAGIGIREDRALITQDMSRFYPHIFVE"
     gene            complement(68214..69002)
                     /gene="ygiD"
                     /locus_tag="OO357_000438"
     CDS             complement(68214..69002)
                     /gene="ygiD"
                     /locus_tag="OO357_000438"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001356237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HCR5885500.1"
                     /translation="MSSTRMPALFLGHGSPMNVLEDNLYTRSWQKLGMTLPRPQAIVV
                     VSAHWFTRGTGVTAMETPPTIHDFGGFPQALYDTHYPAPGSPALAQRLVELLAPIPVT
                     LDKEAWGFDHGSWGVLIKMYPDADIPMVQLSIDSSKPAAWHFEMGRKLAALRDEGIML
                     VASGNVVHNLRTVKWHGDSSPYPWATSFNEYVKANLTWQGPVEQHPLVNYLDHEGGAL
                     SNPTPEHYLPLLYVLGVWDGQEPITIPVDGIEMGSLSMLSVQIG"
     gene            69145..69918
                     /gene="zupT"
                     /locus_tag="OO357_000439"
     CDS             69145..69918
                     /gene="zupT"
                     /locus_tag="OO357_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708851.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZupT"
                     /protein_id="HCR5885501.1"
                     /translation="MSVPLILTILAGAATFIGAFLGVLGQKPSNRLLAFSLGFAAGIM
                     LLISLMEMLPAALAAEGMSPVLGYGMFIFGLLGYFGLDRMLPHAHPQDLMQKSVQLLP
                     KSIKRTAILLTLGISLHNFPEGIATFVTASSNLELGFGIALAVALHNIPEGLAVAGPV
                     YAATGSKRTAILWAGISGLAEILGGVLAWLILGSMISPVVMAAIMAAVAGIMVALSVD
                     ELMPLAKEIDPNNNPSYGVLCGMSVMGFSLVLLQTAGIG"
     gene            complement(69976..70146)
                     /locus_tag="OO357_000440"
     CDS             complement(69976..70146)
                     /locus_tag="OO357_000440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885502.1"
                     /translation="MFIAWYWIVLIALVVVGYFLHLKRYCRAFRQDRDALLEARNKYL
                     NSTREETAEKVE"
     gene            complement(70407..71060)
                     /gene="ribB"
                     /locus_tag="OO357_000441"
     CDS             complement(70407..71060)
                     /gene="ribB"
                     /locus_tag="OO357_000441"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HCR5885503.1"
                     /translation="MNQTLLSSFGTPFERVENALAALREGRGVMVLDDEDRENEGDMI
                     FPAETMTVEQMALTIRHGSGIVCLCITEDRRKQLDLPMMVENNTSAYGTGFTVTIEAA
                     EGVTTGVSAADRITTVRAAIADGAKPSDLNRPGHVFPLRAQAGGVLTRGGHTEATIDL
                     MTLAGFKPAGVLCELTNDDGTMARAPECIEFANKHNMALVTIEDLVAYRQAHERKAS"
     regulatory      complement(71165..71313)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            71435..71725
                     /gene="ubiK"
                     /locus_tag="OO357_000442"
     CDS             71435..71725
                     /gene="ubiK"
                     /locus_tag="OO357_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis accessory factor UbiK"
                     /protein_id="HCR5885504.1"
                     /translation="MIDPKKIEQIARQVHESMPKGIREFGEDVEKKIRQTLQAQLTRL
                     DLVSREEFDVQTQVLLRTREKLALLEQRISELENRSTEIKKQPDPETLPPTL"
BASE COUNT        17963 a        19122 c        17722 g        17080 t
ORIGIN      
        1 gtattgcatt acggtgtgct gagatccatc ggtgcatccc tgtatcccgc gatgagacta
       61 aaatagtcgt gatgtaagct cgtatcagat tcttagtgaa taccacattt gttataatta
      121 agtgaaaaga tacattgctg atttacatta tttggtttac aaaatgtcat cacttgcaga
      181 gttatcgctg acagatacct ttaatctctt actactttcg tgttattgca atacaaccat
      241 ccgtaaagca gggctatttt tagcaggaaa tactcaggtg tcaacattcg aactccaact
      301 ctgaaatatt catataagtt aatgatttta atgattttaa tgattttaat gattttaatg
      361 attttaatga ttttaatgat tttttcattt attcacacct aataaaagta ttcaaattca
      421 gatgattttt ctgctcaaca gacacaaaca ggcaataata ctctccaaag cgaacaatat
      481 ttggcattac gatttaatgc ttcataatca ccaaattatt taaccataaa ttttcttatt
      541 tctaacaaaa tgattggtgt gttgttggag ttaaaataca tacataatat taacatctgc
      601 caacatttca ctttccgcaa gacggatgat ttcgaatcct catccacgtt accctatccc
      661 aaatatcttt attaataggc agtaacgaac actgttatag caagattaac ttattaacct
      721 aacggcatct tttatgtaac aagtggagat aaattgctta aaattgatcc tcgacaaatc
      781 ttaaaagttc tcattattct gcaaaatcag ctttgcgaat aatcttatgt ttttgggagt
      841 tttgggaatt ttgatctact cattgttcta gtgaaaaata taaataagag aaaaaacgcc
      901 attcgtcatg aaagcaacag atgaagcaat gtaataacat actaaaattg atctcatgaa
      961 agtgggtact ttataaccag cacagaagcc aagttgtata aatattaaac aattcgcatc
     1021 gtaaaacgga gactacgtat aaagaagcca atcgtaaaaa cggttccctg gcgagataaa
     1081 cgacagacaa atggaaagcc gggtgatatt taccttcatc cggctttatt gatttacgag
     1141 actaacatcc cggtaaacac atacgcctgc agcagggtga taacgctggc aaaaatcaga
     1201 ctgtgcttca cggtgtagcg gaacagttca gattctcggc ccaccatgcc cgtcgcggcg
     1261 caggccacgg cgatagattg cggggagatc atcttgccag ttacgccgcc gctggtattt
     1321 gctgccacca gcagagtgtc agagacgttg atttgctgcg ccgtggtcga ttgcagtgaa
     1381 ccaaacaggg cgtttgaaga agtgtccgac ccggtaagga atacgcccag ccagccgaga
     1441 aacggtgaga aaaacgggaa catcacgcct gtacctgcca gtaccagcgc cagcgtggtg
     1501 gacatgccgg aatagttggt gacgaaggcg aatgccagca ccatgccaat cgacaatatc
     1561 ggccacttca gactaattag catttcggca aagacgccaa tccctttctt gatgcccacg
     1621 ccgaggatga agatagagat aatcgccgca ataaaaatag cggtgccgcc agccgagaga
     1681 gggtcgaatt taaacaccgc atccattggc gttggttggg cgacaatggg tgccgctttc
     1741 aacacttgtt gatgcaaatg agggatctgg aaattaatca ccagtgaata aaacgcgccg
     1801 cccggagcaa ataacgcttt aaacggcttc atggtccaga tggtgaccag caccgttaag
     1861 attaaaaacg gtgaccacgc acgaatgatt tgccccagac tatattctga aggcacggga
     1921 ccgccagaag atggcttatt taccaccatc gcacctgcgg attgccccat gctgattgcc
     1981 gtttcggtat ttttcggccg ccagacttta aggaataaag cgagtgagac gatactcacc
     2041 agcgccgaag taatatccgg cagttccgga ccaatatagt tagaggtaaa gaactgagtg
     2101 acagcgaagc ttcccccagc aaccagcgcc gctggccacg tctctttcac ccctttccag
     2161 ccgtccatca ttgctaccag ccagaacggc acaagaaccg acaggaacgg taactgacgt
     2221 cccgccattg cgccaatgtg gaacggatcg attcccgtta cctgaccggc gaccagaatc
     2281 ggcacgccca acgcaccaaa cgccaccgtc gcaccaaagc cagcagctcc ttccagcaac
     2341 gcaccaaagg agaaaccaat cagtaacacc tgcaaacgct gatcgtcggt gatggagata
     2401 accgagctgc ggataatatc gaactgcccg ctggcaacgg ttaatttata caggaacacc
     2461 gccgcgacaa taatccacgc aattggccat aatccataaa taaagccata gcccgcagca
     2521 gcaaatgcca tatcaatcgg cattttaaag gcgaatatcg caatcaggat agataatata
     2581 agggttattg ctccagcaac atgtcctttc agacgtaata ccgcgagtgc aacgaagaag
     2641 aatattatcg ggatcagggc gaccagagcg gatagcccca gtcctcccat cggcatatac
     2701 atttgggtcc aggtaaccat attgttcagt ctcttattat ctttatatgc ttgatatact
     2761 taaggttgta ataagcaaaa gaggactgaa ctgtaaaata taggcgttat actttacagc
     2821 aacagtacgc cgctaacgca attgctacct ctggcataac aagtatatcg ggtaagggtt
     2881 tctgttccgc acacgcaggc gcagagtatc gttaagatgt ccatattgtt gttttaggcc
     2941 cgctagtaat gcgctacggg tatttaatat tgttaaaccc tgataatcgc tccggttatt
     3001 tccgggataa atgtactacc gcagttacta tcatagcccc gacaataaaa cttgccgggg
     3061 cttttttgac gctattaatg actttctttt tcgcgtaaac gccaggcgtg taataacggt
     3121 tcggtataac cgtttggctg tttcacgccg aggaagatta aatcgctggc agctttaaaa
     3181 gcacacgagt tagcgaaatt ccccgccatc ggacgataag ccggatcgcc agcattttgc
     3241 tgatcaacca ctttcgccat attctccagc gacgcctgca cctgttcttt ggtcagaata
     3301 ccgtgacgta accagttggc gatatgctgg ctggagatac gcagcgttgc gcggtcttcc
     3361 atcagcgcca cgttgtgaat atccggcact tttgaacaac caatcccctg ctccacccaa
     3421 cgcaccacgt accccagaat cccctgcacg ttgttatcca gctcttgttg gatctcttgc
     3481 gccgaccagt tagcgttttc agccaccgga atagtcagca gatcgtccag cagcggttca
     3541 aattcagcac tgaactcggt ctgggcaatg ttggcttgca cgctctgtac gttggtatgg
     3601 tggtagtgca gcgcatggag cgtagcagcg gttggtgacg gaacccaggc tgtgtttgcc
     3661 ccggcacgca gttggtcgcc cttctggctg tacatgtctg ccatcaggtc cggcattgcc
     3721 cacatgcctt taccgatttg cgctttaccg cgcagcccac agaacagacc ggaaagcacg
     3781 ttattacgtt cgtaggcttt gatccacggc gtcgatttca tctggttttt acgcagcatc
     3841 gggccagctt ccatcaccga atgcatttca tcgccggtac ggtcgaggaa accggtattg
     3901 atgaacgcca cgcggttgcg cgcctgagcg atacagctac gcaagttcag cgaggtccgg
     3961 cgttcttcat ccataatgcc cattttcagg gtattcggtg ccataccgag cattgtctca
     4021 atgcgggtaa acagtttgtt ggcgaacgcc acttcctgcg gaccgtgcat tttcggtttc
     4081 acaatataga cgctgccagt gcgcgagttt ttctgcactt ttaaatcata gagggcaatc
     4141 gcgccagtca tgacgccatc aagaatacct tccgggattt cattgccttc gctgtcccaa
     4201 atcacaggaa tggtcatcaa atgacccacg ttgcggataa acagcaacga gcgtccgtgc
     4261 agagaaattt cagagccatc ggcggcggtg tactgacgat cgtcattcag tttacgtacg
     4321 atttgccgac cgtttttctc cattttctct tgcagagtcc cctgcatcag gcccagcaga
     4381 ttgcggtaca gcaggatttt atcttccgca tcaaccgccg cgaccgaatc ttcgcagtcg
     4441 agaatggtac tgatagcagc ttcgacgata acatcgttga tgtgcgccgg atcgtctttg
     4501 ccaatccgcc cgttggcatc aatttgcagt tcaatatgca ggccgttatt tttcagcaaa
     4561 atgcaggtcg gcgcagcggc atcgccacgg taaccgacaa actgtgctgg agtacgtaac
     4621 gtggtttctt taccattttt caactggatg cgtaattgtt tatcgaccac cttaaacgcc
     4681 accacatcct gatagctgcc gttttccagc ggtagtgatt catcgaggaa acgtcgaacc
     4741 caggcgataa cctgctcacc gcgctgcgga tcgtagccac tgaccatcgc cccttcctgc
     4801 gggatgatgt cgctgccgta taacgcatcg tacagtgagc cccagcgagc gttcgccgcg
     4861 ttcagcgcgt agcgggcgtt cattgccgga accaccagct gtggccccgc ctgactggtg
     4921 atttcgctgt caatgcccgt ggtttccacc gtcacgcgct ccggttgcgg caccaggtag
     4981 cccagttcac gaaggaaaga tttataggcc gctttatctt ttaccggccc cggattgctg
     5041 cgatgccact catcaagcgc cgcctgaatg cgatcgcgtt ctgccagcaa ctgacgattt
     5101 tctggtgcca gatcgtgaac gatctcatca acattgtgcc agaatgcagc agcgtccagt
     5161 tccacgcccg gcaaaacttc tccatccaca aaacgtttaa aattggcgtc aatgcgtaaa
     5221 cggccctggg ttatggtttg actcattgtt tctctcctcg ttttcgctta tttcgccaac
     5281 accgctgctg ccactttcgc gacctgcgca tcctgtgctc cggttaaacc agaaacgccc
     5341 acggcaccaa taatttgccc atcaacaaca accggtacgc cgccttccag cgacgtcagt
     5401 aacggcgcag tcacgaacgc ggtacgtccg ttgttcacca tctcttcata gcccttagtt
     5461 gcacgacgcc ccagcgcggc ggtacgcgct ttctcctggg agatgtaagc cgcaatcggc
     5521 gcgcaatcgt ccatgcgact taacgccagc agatgaccgc cgtcatcggc aacagcaatg
     5581 gaaacagacc agttattttt ctgcgcctct tcctgacctg cggcaataat tgcactcgcc
     5641 atttgctggc taagaatgac tttagttttc atttttttat tccttttcaa gggcttgttc
     5701 tacaatttca atccagtgac gcacagaggt acgaccggcg ctcgccagat gcgtctggca
     5761 accaatgttg gcggtgacga tcatttccgg tttgccgctt tccagcgcat tcattttgtt
     5821 atcccgcagc tggcgtgcca gatcgggatg cgttaacgca tatgttcccg ctgaaccgca
     5881 gcacagatgg ctgtcgggaa cgtccgttaa ggtaaatcca agacgaagta acactttttc
     5941 cacttcgccg ttcagctttt gcgcatgttg tagggtacac gggcagtgga aggccagttt
     6001 tttatcgccg cgaattgcca gtttttccag cggttcctcg cgcagaagtt cgactaaatc
     6061 gaccgccagt tcactgacct gacgcgcttt atcggcatat aacgcatcgt ttttcagcat
     6121 ctgcccatac tctttgacga acgcgccgca accgctggcg gtttgcaaaa ttgcctcggc
     6181 acctgcttca atcgcgggcc accaggcatc aatattattg cgcgcccgtg ccaacccttt
     6241 ctcctgcgca ttaagatgat agtccaccgc gccacaacag cctgcttcgt tagctgacat
     6301 gacgctgatc cccagacgat ccagcactcg cgcagttgcc gcgttggtgt tgggcgaaag
     6361 cgtaggctgg gcgcagcctt ccagcattaa aacccgacgc ttatggcgca gcggcggacg
     6421 cggtttagct ttcaccgttt cagcaggcag ttttgctctg acctgttccg gtaaaaacgg
     6481 tcgcagcacc agccctacct gcgtcagcgc acggaagacc gccggacgcg gcactacctg
     6541 gcgcaatcct tcgcgcagca ttcgctccgg cagtgggcgt ttcactttct gctcgacaat
     6601 atcacgcccg atatccagca aattgtgata gcgcacacca gaaggacagg tggtttcaca
     6661 attgcggcaa gtgaggcagc gatcgagatg ctcctgtgtt ttaagcgtga cttcgttgcc
     6721 ttccagcacc tgtttaatca gatagatgcg cccgcgcggc ccgtccagtt catcgcccag
     6781 aagctgatag gttgggcagg ttgcggtaca aaatccgcag tgaacacagg cgcacaggat
     6841 gctgtcggct tccagcgcgc gttctgccgc atctcttcgg ttaatcgggt ttgcatagcc
     6901 tgctcctcaa agttccgcgt acatgcgacc ggggttaaac acgccgcaag ggtcgagctg
     6961 ctgtttaagc tgctggtgat agcggaataa aggagccgat agcggggcaa agccaccatc
     7021 tccggcacta aagcgggtcg catgaccgcc agcgttgcgg gcgatgcgat ggatttgatt
     7081 gtcatcggct gtcgatttca gccagcgtaa cgccccgccc cagtcgatca gttgctcgcc
     7141 gggtaaatcc atcatcggcg catcgctggg taatgaaatg cgccataagg tacctggtaa
     7201 cgagaagaac ggcagttgtt gttcacgcaa ttgctgccag aactgaccca caacctcttc
     7261 gccacccagc agttcacgcg ctgcttttac cgatccttcg ccgccctcaa ggcggatcca
     7321 caacgcattg tcgaagtaac ataagccact aatgggtaat ggctggagtt gccactcggc
     7381 gatttcactc atggcttctt gcaggctgat ttcccgacgc aggctcagag aggcacgcgg
     7441 tcgcggtaac actttcattg agatttcagt gagcacgcca agacaaccgt agcttccggc
     7501 cattaaccgt gagagatcgt atccggcaac gtttttcatc acttcgccac caaaacgcag
     7561 atgttttcca gtgccggtaa tgatgcgcgt gccgaggaca aaatcgcgga ccgaaccgct
     7621 ccacgggcga cgcggccccg ccagcccgca ggcgaccatc ccgccccagg tggcttcttc
     7681 accataatgc ggcggctcgc agggaagcat ttgcccggcg ctctccagcg ccgcttcaat
     7741 cgccaccagc ggcgttccgg cacgagcggt tatcaccagc tcggtcgggt cgtaattaac
     7801 aatgccgcga tgacaacgca catccagcgt ttgcccggtg actgggcgac ctaaaaaggc
     7861 tttgctattg ctgccctgaa tcaccagcgg cgttttatcg ctaattgcct gattcacctg
     7921 ctccagcagc gcctgactgt aatcacactc gcgtagcatc aaaagcgctc cagttcagga
     7981 aaaggtaaat gaccgtgatg cacatgcatg gcaccaaatt cagcacagcg gtgtagcgtg
     8041 ggaatgtttt tcccagggtt cagcaaacca tcggggtcaa acgccgcctt gaccgcatgg
     8101 aaggtcgtga tttcatcgct gttgaactgg gcgcacattt gattgatttt ttctcgcccg
     8161 atgccatgtt ccccactgat gctgccgcca acttcaacgc agagttcgag gatcttcccg
     8221 cccagctctt ccgcgcgggc aaattcacca ggttcgttgg catcgaaaag gattaacggg
     8281 tgcatgttgc catctccggc atgaaagacg ttggcaacac gtaaatcata ttgctgcgat
     8341 aaacgggcaa tgccttccag tacgccaggc agggcgcgac gcgggatggt gccatccatg
     8401 cagtagtaat ccggggagat acgtcctacc gccgggaacg catttttgag accggcccag
     8461 aaacgtacgc gctctgcttc gtcctgtgcc aaacggacgt cagtcgcgcc cgctttcaac
     8521 aagatgtagt taacccgctc gcagtcttcc tgtacgtcag actccacgcc gtccagttcg
     8581 cataacaaaa tcgcttcggc gtcgacggga taaccggcat gaataaaatc ttccgccgcg
     8641 cggatcgaca ggttatccat catctccagc ccgccgggga taatgccatt ggcgatgatg
     8701 tcaccaaccg caagtccggc tttttctacc gagtcaaagc tggctaacag aacccgcgcc
     8761 acgggcggct tcggcagcag ttttaccgtc acttcggtgg tcacgccgag cataccttcc
     8821 gatccggtga acagcgccag caggtcaaaa ccaggtgaat ccagcgcgtc cgatcccagc
     8881 gtcagcgcct cgccgtccag cgtttgcact tcaattttca gcaggttatg tacggtcaga
     8941 ccatatttca ggcagtggac gccaccggca ttttccgcca cattgccgcc aattgaacag
     9001 gcgatttgtg aggaaggatc cggcgcgtaa tagagattat gcggcgcaac ggcctaggag
     9061 atcgccaggt tacatacgcc cggctgcacg cgcgcgcggc gaccaacggg gttaatgtcg
     9121 aggctctctt taaagcgcgc catcaccaac aacacacctt tttccagcgg cagcgcgcca
     9181 ccagaaagcc cggtgcctgc accacgggtc accaccggta cacgcaggcg atggcagaca
     9241 gccagaatcg ctgtcacctg ttccatttgc ttaggcagaa caaccagtaa tggacgcgtg
     9301 cgatacgcgc tcaacccgtc acactcgtaa ggaatgatct cctcatcggt atgcaggatc
     9361 tcaagtccag ggacatgctc acgcagtgcc atcagtaccg atgtgcggtc gacatcgggt
     9421 aaagcgccat caagacgctc ttcgtacaag atgctcatga gtaggcttcg ctttgttgtg
     9481 ttgtgtggca gctgattttt gcgcgctgct tctgtgaaca gttattaagc gggcttttcg
     9541 ttttcgtcta tctctttagc taccggtcag accatttttt ttccagctct gtgaccttgt
     9601 cttggttaac ttaatgttaa attgatgtaa cataatcact tgcgtgatgt gcgtgttttg
     9661 cgagttaaga acagaaaaat tggtcctacc tgtgcacgag gtccgggaat gaaagatgaa
     9721 cgtcgcccta tttgcgaagt ggttgcagag agtatcgaac ggttaattat cgacggcgta
     9781 ctgaaggtcg gtcagccgct tccctcggaa cgtcgactgt gtgaaaagct cggcttctca
     9841 cgctccgcac tgcgtgaagg gctgaccgtg ctgcgcgggc gcgggattat tgaaacggcg
     9901 cagggtcgcg attctcatgt cgcacggctt aatcgggagc aggacaccag cccgctgatc
     9961 catctgttca gtacgcagcc gcgaacgctg tacgatctgc tcgacgttcg cgcattactg
    10021 gagggcgaat cggcaaggct ggcggcaacg ctgggaacgc aggctgattt tgttgtgata
    10081 acccgctgtt atgaaaaaat gctcgccgcc agtgagaacc acaaagagat ttcgctgatc
    10141 gaacatgcgc agttggatca cgctttccat ctcgccattt gtcaggcttc tcacaatcag
    10201 gtgctggtgt ttacgctgca atcattgacc gatctgatgt ttaattcagt gtttgccagc
    10261 gtaaataatc tctaccatcg accacagcaa aaaaagcaga tcgatcgcca gcatgcgcgg
    10321 atctacaacg cggtgttgca gcggctgccg cacgtcgctc agcgcgcagc gcgcgatcat
    10381 gtacggaccg tgaaaaagaa tctccacgat atcgagctgg aaggccacca tttgattcgc
    10441 tcggcggtgc cgctggagat gaacaaggtg ggaatgtgag cagagtttta ccctatcccc
    10501 aaggcgaggg gaccgcccct gcacttaaca ggatctggtg ttaaatctgt tcatctatac
    10561 cctctcccga atgaccgccc ctgcgctaaa caggatctgg tattaaattt gcttttcata
    10621 caatcggtaa cgcttgtacg gctccgcccc aatgcgttcc agcatgttat tcatgcctgt
    10681 attggtttcg aggatccatg acatctccag cgcatcgatc ttccggcggg caaacggatc
    10741 gcgtaaggct tcaatcaata acagcgcaat caccgggccg atgcggctga actgatactc
    10801 gtcgcgcacg cccatcagcg gtactcgcgc agttcgcaca ccgctgactt tcaaacgcca
    10861 cagcaatttt gcccagccga agggaaagag cgatccgttc agatcggcaa tcgcctcgtt
    10921 gatgttaggc aagccacaat aaacgcgcag ggtgcagaat caatctcagc gatgtagatc
    10981 atatcgtccg gcaccagata tttaagttgg tcgcccatgg tcgcgaattc atgttcggta
    11041 aacggcacaa atccccagtt gtgctgccag ccagagttga aaatctcacg caggatctgc
    11101 atctcttcgg caaaccgctg acgattgatg cagcgaatgg tcaccttttt gcgcacctga
    11161 tccatcagtt tttttagcgc cggagagaaa gtgagatcgg ttcgctgcat ccaccacgcc
    11221 agtaaatcaa tgcctttgtg ataacccagt tgttcaatat gcgcggcata ccacggtttg
    11281 ccgtgtggca tcatcgcaca gggtggtgtg tcaaaacctt caatcagtaa tccgctttcc
    11341 tgattgatat tcaggctgaa aggcccgctg atcttacttg caccttgtga cttcaaccac
    11401 gcttccgctg cgccaaacaa cgcggcaaaa tcctgcggat catcaatggc gtcaatcatg
    11461 ccgaaatgac cggtatcttc accgtaacgc tcacggtgca aggtatctat ttgcgcggta
    11521 atacgcccaa ctatctgccc cgcttttttt gccacccacg cctgccagat gatatggtcc
    11581 gtccccggat ttttcgcaga caaatgctcg ttgcgttcaa tgaataaagg gggtatccag
    11641 tttggatcgt cgggataaag tgatgacgga aaagcgataa atgccttaag gtcattttta
    11701 ttaattactt tttcaatttt aatcatcagc agatcgcatt ccatatctct gtggataaca
    11761 aataattaat ggtgaaaaac aggttgcaac agcaacgagc ggcatcttaa taatcaactc
    11821 aatgaataca aacgacgaat aaatagtatt aattaatgat tatgataagg tcagaggcta
    11881 cacagaaacg cctcaatgcc ttttgtcaat ttaaggcgac cggacaaggc ttcttcttaa
    11941 tatattcata aattaaatat cattcataat aaaaacaaaa aacttaaact cattatttaa
    12001 atcaatgttc attttcacac cgatgaaaat actatctcat ttatctttta tgcctatata
    12061 atccaccgca ttattgtcat tttatttttg atatccaatt ttttcgtggt gtatatgtct
    12121 aataaaatct ttacgcattc cctacctatg cgctatgccg attttccaac gctggttgat
    12181 gctttggact acgccgctct gagtagagcc ggaatgaatt tttatgacag acgttgccaa
    12241 cttgaagatc aactggaata tcagacatta aaaacgcgtg ccgaagctgg tgcgaagcgg
    12301 ttgttatcgc tgaacctgaa aaaaggcgaa cgcgtggcgc tgattgccga aacgagtagc
    12361 gggtttgtag aggctttttt tgcctgtcag tatgctggct tagtcgccgt cccgttggcg
    12421 attccaatgg gcgtcggtca gcgggattcc tggagcgcca aactccaggg tttactggca
    12481 agttgccagc ccgcagccat tatcactggt gatgagtggt tgccactggt caatgccgcg
    12541 acgcataaca accccgaatt acatgtttta agccacgctt ggtttaaggc attaccggaa
    12601 gccgatgttg cgctccagcg tccagttccg aacgatatcg cctacctcca gtacacctcc
    12661 ggcagcaccc gttttccccg tggcgtcatt atcacccatc gcgaagtgat ggctaatcta
    12721 cgtgctataa gccacgatgg cattaaatta cgccctagcg accgctgcgt ctcctggcag
    12781 cctttctacc atgatatggg actggtcggc tttctcctgc cccccgtcgc cacgcaactt
    12841 tcagtagatt atttgcgcac tcaggatttt gccatgcgtc ctttgcaatg gcttaaattg
    12901 atcagtaaaa atcgcggcac cgtttccgtt gcgccgccgt ttggctatga attgtgccaa
    12961 cgccgcgtga atgaaaaaga tctcgctgaa ctggatcttt cctgctggcg cgtcgctggt
    13021 attggtgctg agccgatctc cgcagaacaa ctccatcaat tcgctgaatg tttccgtcag
    13081 gttaactttg acaataaaac tttcatgccg tgctacggac tggcagaaaa tgcgctggct
    13141 gtcagcttct ctgatgaagc ctccggggtt gtggttaacg aagtggatcg cgacatcctc
    13201 gaatatcagg gtaaagccgt cgcgccgggt gcagagacac gcgccgtatc gactttcgtc
    13261 aactgcggca aagcgttgcc ggaacatggt attgaaatcc gcaatgaagc aggtatgccg
    13321 gtcgcggaac gtgtggtagg ccatatttgc atctccggtc ccagtctgat gagcggttac
    13381 tttggcgacc aggtttcgca agacgagatt gccgcgacgg gctggttaga caccggcgac
    13441 ctcggttatc tgctggacgg ttatctgtat gtcaccggac gcattaaaga tctgattatt
    13501 attcgtggcc gtaatatctg gccgcaggat attgaatata tagcggaaca ggaaccggaa
    13561 attcattctg gcgatgcgat tgcttttgtt accgcccagg aaaaaatcat tttgcagatc
    13621 cagtgtcgga tcagcgacga agaacgtcgc gggcagctta tccacgcgct ggcggcacgg
    13681 atccaaagcg aatttggcgt gaccgcggct atcgatctgt tgccgcccca cagtattccc
    13741 cgaacgtcct ccggcaagcc tgcccgtgcg gaagcgaaaa aacgttatca gaaggcttat
    13801 gctgccagtc ttaatgtgca ggaatccctg gcataaatca aactgtcgcg gtgacgggcg
    13861 ctaccgggtt tatcggtaaa tatattatcg ataacctgct cgcccgcggc tttcacgttc
    13921 gcgcattgac gcgtactgcc cgcgctcacg tcaatgataa tctcacctgg gtgcgcggtt
    13981 cgctggaaga tactcattca cttagcgagc tggttgccgg agccagcgta gtggtccatt
    14041 gcgccgggca agtgcgcggg cacaaagaag agattttcac ccgctgtaac gttgacggca
    14101 gcctgcgcct gatgcaagca gcaaaagaga gcggcttttg ccaacgtttt ctgtttatct
    14161 cttcgctggc ggcgcgccat cccgagctct cctggtatgc aaaatccaaa tacgtcgccg
    14221 agcaacgact ggcagcaatg gcggacgaaa ttacgctggg cgtttttcgc ccgacagccg
    14281 tgtatggtcc cggcgataaa gagttaaaac cgctgtttga ctggatgcta cgcggcctgc
    14341 tgccacgact tggtgcgcca gatacacaac tctcttttct tcacgtcacc gattttgcgc
    14401 aagcagtggg tcagtggtta agcgccgaaa ctgtacagac gcaaacctat gaattatgcg
    14461 atggcgtcgc cggtggctat gactggcaac gcgtacgaca gcttgccgcc aaagcccgtt
    14521 gtggttccgt gcgaatggtt gacattcctc tgccggtact cacaggcctt gcggatatca
    14581 gtaccgcgtt gagtcgcctg gcaggtaaag aacctatgct gacccgctcg aaaattcgtg
    14641 aattaaccca cgccgactgg tcggcaagta ataaccgtat ttctgaagat atcaattggt
    14701 ttcccgggat tagcctggaa cacgcattac gcaacgggct attttgattg tcttgtttta
    14761 aaggtgtgtt aacgtttcta tggtaaatcg tgaaatagta atggattaca tcctttcctg
    14821 tttacaggat ttggtcgaaa acggtgtgga aattaagccc gacagcgatc tcgtcaacga
    14881 tcttggcctg gagtcgatca aagtgatgga tctcttaatg atgctggaag acagattcga
    14941 tatttccatt cctattaata ttctgctgga tgtcaaaacc cctgcgcaat taatggaaac
    15001 tctacttcca tggctggaga ataaataatg gggctatacg ataaatatgc gcgccttgct
    15061 ggcgagcgtc tgcaatttag tgacaacggg ttaacaccgt ttggcacctg tattgatgaa
    15121 gtgtattccg ccaccgaagg ccggatcggc aataaaaaag tgattctggc cgggaccaat
    15181 aattatctcg gtttaacctt taatcatgat gccatcagcg aaggtcaggc cgcgttagcg
    15241 gcccaaggca ccggcaccac cggttctcgt atggcaaacg gcagttatgc gccgcacctg
    15301 gcgttagaga aagagattgc cgagtttttc aatcgcccta ccgctatcgt ttttcaaccg
    15361 gttataccgc taatttaggc gtcatcagcg cacttgccga ccataacgcc gtggtgttac
    15421 tggatgccga cagccacgcc agtatttatg acgtctgttc tctcggtggc gcggaaatta
    15481 ttcgtttccg ccataacgat gctaaagatc tggaacggcg catggttcgc ctcggtgaac
    15541 gggcgaaaga ggcgattatc atcgttgagg gcatctacag catgctcggt gacgttgcgc
    15601 cgctggcgga aattgttgat atcaaacgtc gtctcggtgg ttatttgatt gtcgatgaag
    15661 cgcattcgtt tggcgtgtta ggcgcaacgg ggcgcggact ggctgaagct gtcggcgttg
    15721 aagatgatgt ggatattatc gttggtactt tcagtaaaag tctggcctcg attggcggtt
    15781 ttgctgtcgt ttcggaagcg atggaagtgc tgcgctacgg cagccgcccg tatattttta
    15841 ccgcctctcc ttctccgtcc tgcattgcga ccgtgcgctc gtcgttaaga actattgcca
    15901 cacaaccaga gctgcggcaa aagctgatgg ataacgccaa ccatctgtat gacggtctgc
    15961 aaaaactggg ttatgagtta agttcacata ttagcccggt agtgccagta ataatcggtt
    16021 cgaaagagga aggtttacgc atctggcgcg agcttatctc gccgggcgtg tatgtgaatc
    16081 ttatcctgcc gcctgccgcg cctgcaggca tcacgctgct gcgctgtagc gtaaatgctg
    16141 cccacagtcg tgagcaaatc gatgccatca ttcaggcatt tgctactctg aagcagtaat
    16201 gtgagcccgc cgtggtgttt gcgccacggc gggtttaatg ttgtctgtca taccaataca
    16261 ccaggctcaa cgccaccaac acaaacgcta ccggtaaacc gagcgcggta acaggtgcgc
    16321 ttaacgcaaa tagcaatccc agattaacca tgatttgata gctgatccag gtgagtaagc
    16381 ccacgatgac gcctacagcg agacggctac ccatcccagg cgaacgcggg gcactaaagg
    16441 taaacggcac agccagcaaa atcatcgcca gggtcaggat gggttgcccc agtttttccc
    16501 acagtgcaag gtggtattcg ctgctgggtt gcccggtttc ctgcaaataa tggatgtaat
    16561 gattaagctg acggacagaa aaagtattcc ccggcattga taactcttca agattcatac
    16621 tggtgaaggc cgattgccag gcaagattat ctaatgtttc cagcgtttct ttgccattca
    16681 gccatttttt atgattcaca ccatgcagga cccacgtttt gtcgtcttca atggtggcac
    16741 tgcgtgcata aatgtaggat tcaagcgaga gatcgtcgcg ataatgaaat atctccacgc
    16801 ccaccggctg gccctgctcg ttcagcgatt tcaccgtcac aaattcatta ccacgcctgg
    16861 cccacagcat attgccggta atgtcatcgt cctcccccaa cgcggtggcg gtcgatttga
    16921 tttgcaacgc ctgttgctgc aatggcgacg ccacccactc atctatcgcg cctaacgaaa
    16981 cagtccacaa tatcccggca accagcgcca ccagtgcaat acggaagata gaaaaccccg
    17041 tgctgcgaat ggcggtaagc tcactgtttt tcgataactg ccccagtcca acaataccac
    17101 ccaataacgc gataaacggc gaaagttcga ccagtgtgcg cggtaaggtc attagcacca
    17161 ccagcaccgc ctgagtccag cgataaccgc ccgggctgac accatccagt tcgttaatca
    17221 ggttgaaggt ggtaaaaagc ggcagcaata gccctgcagc ggcggcaaaa ccgagaaaga
    17281 gatgacggat taaatagcga ctgaaaacat tcatcgggaa aatttctgca gcaaagagaa
    17341 gtcacgtgcg accagtatca gcaggcccat tagcatgagt ccgggcacta accacaaacc
    17401 aggaatgagg ggaatcgcgc cgttagccac cagtgtacgg cagacgtcgc cgccataaaa
    17461 aatggcaata aacagcaacg tcagtggcaa taacgttgaa aatcgccctt gccgcggttt
    17521 tacccgactt aatgaaatgg ccagcagcgc catcaacaat gcggtcaggc cacggctttg
    17581 tcgccattgc agttcggcat gatcggcagg aaaggcggag cgcgccagct ccgtgaccga
    17641 tttcgctttg cgtttgacgt tcgggctttg atccagcgga ttcagatgta attgcagatt
    17701 acggtagatc tgttcgttgt cgtcacgccc ctgatgatcg agaagatagg cagtgccgtt
    17761 gtgcaacata acggtcggtt tctcgggtga tgggtcaacc acatcaaccg aaccggcgcg
    17821 gaaaatacgg gttcgattgg cagtagaagt ataaatcagc gcgtcagtca ggcgattatt
    17881 atcctgatca accgtctgcg aaaggatcat tcgtccgtta tcgttagtgt taaatttctt
    17941 tgcccgcaac tggcgaacat ccagctccga ctgcgactgc tgctccagtt gataaatctg
    18001 cgcataagcc cacggacgac catacatcga cagaagggtg acaaaaatac ttagcggtat
    18061 cgccagataa agaacggctt tgtacaagcg tcccggactg ccccccgccg cagagatagc
    18121 ggtaatttcc gagtcggtat acatctgccc tagcgtcacg ccaactgaca catacagacc
    18181 aacaggtaac aacatctcca gtgcaatcag tactttgtag aaaacgatat ccagcacaac
    18241 atcaagagct aatgtcccat ttgccgcttc ggtcagataa cgctgtgcgg agtagctggc
    18301 gaaaataaag atcagaaaac cgacgataat cagcaccaga cggcgcgtac cacgcatgat
    18361 gtagtgttca accagcttca tctcactaac gccctcaatc ctttgagttt tttattcatt
    18421 gatcagtttc tgcgccaacg caatgacatg atctacctct tccggcgtca ggctgccgtc
    18481 ctttgcccac tgaatttgcc cggctttatt cagcaccaga atggttgaac tctgctccgg
    18541 caagcgccag gcaacgcgtc cctgtccatt gccgtcaata acgaattgcg cccacggata
    18601 gcggcgttta tttttttcga ttttgccaac aacaaaatag ccagtaccaa agatggcatc
    18661 atcggtgttc acaatggtgg tgggctgaaa acgatcctgc ggaaagtttg ccgcttcaac
    18721 cgctttaatt aacagtgagt tctttttctt cgccgactta cgcccggcga tatactgaat
    18781 aatgcggact tttcccgcta attgtgtgct gttccacttt tggtagtgaa tttcgtcgtc
    18841 gctattcaga agcagctcgc cgccttcctg gatataaaca ggggtaaccg ttttgccagt
    18901 gacgaaattg tgtgcctgca cacccggggc taagagcatg ataatcaacg caataatcaa
    18961 acgagatgac atcattgctc cggctatagc ggaaaacgcg aaatcagcag ctctaagcgc
    19021 tgacaaatat cgctatccag cgccaacggt gcataatacg actggcggtt ttccatatca
    19081 tccgccacca tgtcgtcgtg tttatctccc atcaggccat taatggcggc gaggaaacgg
    19141 gaaatctgta acgcctcttt attttcagga tcaagcgatc ttaaggtgtc gtaataagaa
    19201 acctgctggc tgatgatttt tcgcacatca tgaaattccc tgccggttag tcgcggtttg
    19261 gcaataatct ctttcagggt atgcatttgc ttttgccgat aggcaataaa actgcttacg
    19321 ctttctaact ggctatggcg taatgaatag ttcaccaacg accagaccgg agagcttaaa
    19381 taaatccgta acaggttgcc gtaatacgac acaatatttt tcttaccgtt acgaaagccg
    19441 tcctgaaaac gccccagaaa aacggtcgtt ttgccgaata aaaatccggc ctgatgcttc
    19501 tttaaataca gccgttgcgc aaaacgcagg tgcttgtagc gcgctcgcat gtatttaaat
    19561 tgtttgcgct catcagcagt cagctcatcg cctttggctt gtttgcgaac taaacgcagt
    19621 aattcctgac gatcaacgcc attgagccag aattgcaggc ataacgcgta attatcatga
    19681 atgatttcgg tggtacaact tagtgcaata gtcggcggca aactaacctg tgcatcaacg
    19741 acatcatctt cttcaatcgc aacaaataga cgctcgctcg cctcaagcgg gaaatatttt
    19801 ctcatcatca ggatttctct ccggctgcgt tataaagcaa ggaatggcgc tgaaaaaatg
    19861 cctttaaggt tggaattaac gagagcgcca cgtccagaca ttgcccaccg aagtaagcaa
    19921 acgctgctcc cagcacacca aacagatgac agaacatgaa cagcaatacg atgtagccga
    19981 ttgaggcgat ggtttgcgcc acgagaaaag cgcgctgttt tcccgccatc aatagcaatg
    20041 actcctgcgg gaagcccagc atcgaaatca caattgcgcc cagcatcacc tgaatcaggt
    20101 catacgcttc gagatactta acgccaaaca ccagcgaaat gagcggcttg ccgacgatca
    20161 acaccgcgag cgccaccaga ataccgattc cgcccgccag taaaccggac ttcacaccta
    20221 acaaccacgg tctggtggtg cgcggatcta aacgcatcac ctctgggtaa aaacttttac
    20281 ccagcaaacc cgctggcgtt ccggcggcgt cgaagaatgt catggcgatt ttaaataacc
    20341 cggcggcagc gggtcctaac acgatcccca ccaacactgt gctgcacgag ttacgcgccg
    20401 accagatgga gtgggcaatg ttggttgacc agacaaaact ccacgcgcct ttaatgtgtc
    20461 gggcagactc aaacagattc aatttgaagg cgttatggat atttctacgg cgtaattcgc
    20521 gcgcggcaaa ccaccagtac atggtgccgc caaccagatt cgacacgtac caagcaataa
    20581 caaaacccac aaaaccaaag tcaaaatacc aggctgcgac gctccccgct gcgcgcagaa
    20641 aaggtttcgt cgcctgctgt acagcaatca aatcaaagcg atctaccgca cgcagaatgc
    20701 cggtcggcgt ggaggaagcc attgaaggaa tgagcgtgca atacagcgcc gccagccaaa
    20761 agctttggtc atccagacct aatgaatggg aaaggaatgg cagtaaggca atgccgccga
    20821 caatcgccac cgcgccgctg acgatatcca gcgagaagga aaatgagacg acattgcgaa
    20881 attgctgcgg attattgttg gtcaaggctg gcgttccgta ctgaaccacc agctgccatg
    20941 tctgaaactt aataaaatcg ctgatcgact tggcgtacga ttgcacaatc accagtacac
    21001 caaacatggc gggcgtcatc cctttaccgg cacacgagag cgccaacagc cccagcaagg
    21061 cgctctcgac attactggag cctaaccagg cgctattgcg aataatggtg cgaaacgcgc
    21121 catctgcaaa ccaatgtttg atgttaaaac ccgccaattc agcctgacct tatcgttgat
    21181 agtaaaaaat atcccgccag ccttaagtta aacttcggcg gtcagaaacg atggcaacca
    21241 gagaaaccgc cttctgtacc tgttccagca cttcgctgta gggcgctctg gaatcaatct
    21301 caagaatttt tgtgccgtta tagccaatcg tcgacatgac gccgattttg tcctgcagct
    21361 cggcatactc atggtcaggc ttgcgggaga tggcagtatc aatatcaatc cccaaacgaa
    21421 tgattaattc aggacggtat tgcgccattt tttggtataa actccgttcg cgctgcgcca
    21481 gaaacatgct gattttcccg gtcgcacgtt cgacgccgat ccccggtccg tcataataaa
    21541 agcccgaaat ttcagcctgc gggaagcgat cgctgaccac cagaacgcca ctttgcgcca
    21601 gtcgctgaac cttgcgtaga ttcgccattc ttcgcagcga gaagcagtac ataatcaccg
    21661 ccgcccatag cgccggagat ttggttttca tgctttgggt tttcgaggat ttggccgcca
    21721 atcgccgttc cagccagacg ccaaccaacg gcaatcgttt gattttgtcg ccgtcttcgc
    21781 cggagagcag ccccagatag cggcgctcgg tttgccagtg ttgttgcagc gatttcacca
    21841 ggtcggtggt cagcgtggat ttaccggtgc catcacaccc gaccaccgca atcagccccg
    21901 gaatgtagtt gggctgcggc gcggttgtac tattaacagt ttgtgtttgt agtgcatcca
    21961 ttcgttaaat catcccctat ccattttggt gatcaggaaa gcgcggcgtg aattgcgcgt
    22021 agtgacgctt gcataatttc atcggctggt tgccgaccat ccagttcaag gatcttggcg
    22081 ccattgaatg tcagttgcgg cgtaacggcg attttttcct gtaacgctgc cagttggtgg
    22141 tcaggtttac gcgcaaacgc ggtttgttca tcaatgccaa gacgaatcaa caatacgggt
    22201 aaataagacg ccatccattg gtacagcttc aactcgcgct gccttaacat ttttatccaa
    22261 ccgttaccgc ccgtggtttt tgccaattgc gggccatcaa agcgaaaacc cggcacttca
    22321 acctgcgggt agcggtcggt gatgagcaga tagccttgct ggcttttaca caacattttg
    22381 cgaaacttgt acgcccgcca gcaggaaagc agatagataa ccagcgcagt aatattgcca
    22441 ggcggagtta agggcttttc gtgtacatgt gccgctttac ttcgcagata acacccacaa
    22501 ggtgcgccaa taacagggag ctgtgaaatc cattcgccaa ttcgcccgga cgattgcccg
    22561 agataaatgt gttctgttgg cattcttgct gccagttcat ttaccaggct tgccgtgagg
    22621 gtcgatttac ctgaaccatc acatcctaca atggcaataa cacgcactgg cgtggaatta
    22681 attattgaca tattacgttg attcacgaaa aacccggcag taaataatgt attgaatatt
    22741 acgtggtccg tgctcagaat atccgttcaa ccttattgtg acaatgacta attgtgacat
    22801 tgacctgaat tacgctttca taaaaacata ttaaccaaat aaatattttt aatggatatt
    22861 taaattaaag gatatattca tgcagtcaat aacacctcca ttaattgccg ttattggtag
    22921 cgatgcttca ggcaagtcaa cggtgtgtga acatcttatt accgttgtcg aaaaatatgg
    22981 tgctgccgaa agagttcatt taggaaaaca ggccggaaat gtcggtcgtg cagtgacaaa
    23041 attacctttg atgggaaaat ccttacataa aacaattgaa cgaaatcagg tgaaaacagc
    23101 aaaaaaattg cctggaccag ttccggcgct ggtaattaca gcgtttgtcg cccgtcgctt
    23161 actgcgcttt cgtcatatgc ttgcctgtcg tcgtcgcggg ttaattgttc taaccgaccg
    23221 ttatcctcag gaccaaattc ctggcgctta cgatggtacg gtgttcccac ctaacgttga
    23281 aggtggtcgt tttgtctcat ggctggcaag ccaggaacgt aaagcgtttc actggatggc
    23341 gagccataag cctgatctgg tcatcaaact caatgttgac cttgaagttg cctgtgcacg
    23401 taaacccgac cataaacggg aatcgctggc gaggaagatt gccataacgc cacagttaac
    23461 ctttggtggt gcacaactgg ttgatatcga tgccaatcag ccactggaac aggtgttggt
    23521 tgatgcagaa aaagcgatta cggattttat gaccgcgcgt ggttatcact agtcaaaaat
    23581 ggaaatgccc gatcgccagg accgggcatt ttcaggaagg ttaaatcaac tgcaatgcta
    23641 tccagtacag cccaccagaa agaaaaattg ccgccggtaa agtaaacacc cacgccatca
    23701 ggatgctggt taccgtttta cgctgtaacc caccgccgtc caccaccatc gtccctgcta
    23761 ccgcagacga gaggacgtgt gttgtggaga caggcatccc aatataactg gcaaggccga
    23821 tagacactgc cgccgtcatt tgtgccgcca tgccttgcgc atacgtcatg ccgcgcttac
    23881 caatcttctc accgatggtc atcgctacac gacgccagcc aatcatggtg ccaatgccga
    23941 gcgccagtgc taccgccatg atgatccaca ccggagcgta ctcaatggtg cttaacatat
    24001 cgctgcgcag ttttttcagc aggttctggt cttctttact gacgcctggc agtttcgcca
    24061 gcttcgcgga ggtatcggag atgcacagca taatgcggcg cagctggctg cgctgactca
    24121 cgcttaacgg ctcgtaactt tccatattgc ctggcagcat cgttttaacg cgcgcaatcg
    24181 catcaaaggt atttgccgga tgacagtgaa actctgttac ttgcgtgcca tcagtcgatg
    24241 ctgcaggcaa tggaggttcc atcgcaatca acttctgcgg cagttcagga tgctgttgca
    24301 ggtagtgttc gaagttggta acggcatcgc gggtacgggt aatttcatag ccggacgcat
    24361 tcatattgac gacgaagcca gcaggggcaa tccccaccag taccagcatt accaggccga
    24421 tccctttctg cccgtcgttc gcgccgtgcg aaaacgccac gcccgcagcg gaaacaatca
    24481 gcgcaatacg cgtccagaat ggcggtttac gtttgccctt tttctttttg cgatcttccg
    24541 gaatgcggtg aatacggtca cgctttttcg tcccactcca gaaacggcgc agcaggaaaa
    24601 tcagaccacc cgcaatgacc aggccgacga taggggaaac aatcagcgag gagaaaattt
    24661 tggttacttc acgcaggttt aacgcatcca tcaccgatga gccggttaac agcgcgttgg
    24721 ttaaaccgat gccgataatc gcaccaatca aggtgtgcga actggaggcc ggtaaaccaa
    24781 agaaccacgt tcccaggttc cagataatcg ccgccagcag catggaaaag accatcgcca
    24841 aaccgtgagt tgaccccata ttcagcaaca aatcgattgg taacatatgg acaatggcat
    24901 aggcaacgct aagtccgccc aataacacgc caaaaaagtt aaaaaatgcc gccatcacca
    24961 cagcaagttg tggctgcatg gcacgagtat aaataacggt tgccaccgca ttcgccgtgt
    25021 catgaaaacc attgattgct tcgtagaaca acacaaatgc cagagcaagt aataacaaaa
    25081 gccccgtata tatatcaagg ccaacaaata aatttagcat atattagatt acgccatttt
    25141 gaatttacga acggacgcat tatcagtgac tttaacggca tgggcaaagt gaaatatcat
    25201 tttttaacga taaaaagagt gttatttctt gtgtatcaga caataagcat tcattcaaaa
    25261 cctttagaaa taatcaaaac acaggtattc cactggttaa tatattcaca tatgaaatga
    25321 ataaatattg gaatatataa atattgaata ttttgattaa tccccgtact aattattctt
    25381 cataatcagt acggggttgc tacaacatgt attacttttt caccacaatt aacggttcaa
    25441 tatcactctc ttttttgatg accagtgatt catcaccgcg caaacacgtc ccaccgtagt
    25501 tgccgccaac ggtgaaggta catacctgaa tgtatttacc gtccactttc ggcaaacacc
    25561 acagttgctg atagatgttt ttctgctcgg caaatttacc gctggttttg tccagcacct
    25621 cttcatgatg gctgaccagg tcgatattgc taccacagcg accggcgatc ggtttcactg
    25681 cgtaacctgt tttcaccaat tcatcattaa cagtgaaatc ggtatccagc aggtaacgat
    25741 ggtgcgggaa cagcgaccag aggatcggca gaatcgcttt gttgccgggg atcaccgtcc
    25801 acagcggctc aaagaccagc acttccgggc gcagcaatac gtcgataaga cgcacttcgt
    25861 tttgcggatg accggtacgg attggcaccg cagcaaactc acggtcgcta acttcacgaa
    25921 tctgatcaaa cgcggtttcc cacgcccagg ttttccacac gcagttaacc agtcgccctt
    25981 ccccatcaat cagttgcccg gcagcatccc agcccagttc atccagcccg cgcaagatac
    26041 gcgtttcaaa gcccgcctgg tgcagcgcct gctccataaa ctgcgcgtga tagttttcct
    26101 cgatatcttt gtcctgcatg atatggacaa acggacgtgc acgactgtgt ttccaggcac
    26161 cagccagttc gttaatcagc ccttccgccg gattgaagcc gttgccttta tagccctgct
    26221 ccgcccaacg ttcgaggatc aagcccgctt cggtatgaca ggaggcggag tcggcgttgt
    26281 attcgtaaac cttcaggcca cgctcatcca tgcagaaatc catacgacca gtgatcatat
    26341 ggtgacggcg acgctgccag gagagacgca aacgtggcca gaggattttc ggaatgtcga
    26401 acagcgccag caggttgtca tctttcagca ccttgtcggt tgcgtgaaga tacatcagat
    26461 gcagctcgtt ggtggcttta attagctcct gctcggcact ctcggtaatg gtgtagtaat
    26521 gatacggatc ctgattgatc acctgaccgt tggcctgcac ataggcgttt tgtagcggat
    26581 ctttttcatc cagccattta ccgtcaaact ggcctttgtt ttccagacgc gctccactga
    26641 ttttcagcag ctcgcctgca atttccggct gcggtaagct gtattcagta tcttccgtct
    26701 ggatcatcca gcccagaatg gtggtgtcat caaaagtgtc tttaagggta tagcagccgt
    26761 tttcgaccac catctccagc tcgcgcgtcc actgttgccc ttgcggcaac ggggaatgaa
    26821 tcacgttctg ttccgcaata cggactttgt tgccatgcaa ttgggtaatg atggcgacat
    26881 ggccagtgtc tttaaattca ccgcctttat cccagataag aagcgcaccc gcgaccggcg
    26941 cacgcggcga gccgttagga aatgcctgca atggcaggat gttgtcatta accacttctc
    27001 gcaggaagcg cagcgagaaa atctcccacg ccatacccac gtcagtaaag accacaccgt
    27061 aattcagaaa gagaaaacgg cgagcaaatt caacgcattg ccacttgtgg cccatatatt
    27121 cgtcgtcgat atagctacgg aatacggcgt catcttcgta ttcctgcgga tcgagagaac
    27181 tgtaatctga agagtagatt gctaccccac ctggggcgta gcccaataat gtcccgaacg
    27241 gggcatcctg gctggtcgtt cctttgctca tcactttacc tttaacaata caacctaagc
    27301 agttggcgta attgttgtgc tctgattacc tgctcagcga cattaaccgg acagaggtca
    27361 gactaatcat acacctcatc gcagcggctg gcgcaaaggg ttaaaaaatt cacattctgt
    27421 acagcaagtg acctgctaca ctgcttcaac actaccactc agaaggcaac tcactatgac
    27481 agacaatact tatcagcccg cgaaagtctg gacgtgggat aaatccgctg gcggcgcgtt
    27541 cgccaatatc aatcgcccgt tttctggtcc gacgcatgaa aaaacgctgc ccgttggcaa
    27601 acatccattg caactttatt cgctgggaac gccgaacggt cagaaagtaa cgattatgct
    27661 tgaggagcta ctggcgctgg gcgttactgg tgcagagtac gacgcctggc tgattcgtat
    27721 tggcgatggc gatcaattct ccagcggctt tgtcgaagtg aacccaaact cgaagatccc
    27781 ggcgctgcgc gatcatacgc ataatccgcc gatccgcgtg tttgaatctg gttcgatcct
    27841 gctttatctg gcggagaaat ttggctactt cctgccgcag gatctggcaa aacggactga
    27901 aacgttgaac tggctgttct ggttacaggg tgccgcgccg ttcctcggcg gtggttttgg
    27961 tcacttttac cattacgcgc cggtgaaaat tgagtacgcc atcaaccgct ttaccatgga
    28021 agccaagcgc ctgctcgacg tgctggataa gcaactggcg cagcataagt ttgttgcggg
    28081 cgatgagtac accattgcgg atatggcgat ttggccgtgg tttggcaacg tggtgctcgg
    28141 cggcgtgtat gatgccgcag agtttctgga tgcgggaagt tataaacatg tgcaacgctg
    28201 ggcgaaagaa gtgggcgaac gtccggcagt gaaacgtggg cgtattgtta accgcaccaa
    28261 cgggccgctg aacgagcagt tgcatgagcg ccatgacgcc agtgattttg agacaaatac
    28321 agaagataag cgtcaggggt aagggttggt gttcgtcgca gcaagccatc caggccggat
    28381 aaggcgttca cgccgcatcc ggcaatcgtg cacaatgcct gatgcgacgc tgccgcgtct
    28441 tatcaggcct acaaaagcta atcatcacgt caggcctaca aaagctaatc atcacgcgct
    28501 ggtaatgtcg taatccattt ggcgcagtgc gtctaatgtg gctttggctt catcttcgtc
    28561 gatgatgctc atggcaaatc cgacgtgtac cagcacccac tggcccagca gatcggcagg
    28621 gttaccttcg caaatcaggg cgatattcac gtcgcgcttg ataccacata cttcaacctg
    28681 cgcaagctgg tgaatatctt caccgacagc cagcacctgg cctggaacgc caatacacat
    28741 tgttaactcc ggttatttcg attgccggac aaacgcgaaa aagaaccttt agcgggtgtt
    28801 ccctctcacc ctaaccctct ccccggaggg gcgaggggac cgtccgtgcg ctttttaggc
    28861 gttgtcctca gtcttattca acttcaatac ttttgacgat cagcgaatcg ccggtatcga
    28921 cacgcaaccg ctcgccgtga cagagcggac actgcgcatc gtgctgatga atctccacca
    28981 cctggctgca atcccagcac caggcctggg cgggtttata gacgatatgt aaatcgcacc
    29041 cttgcgccac cgttccgtgg cagacaattt caaaactaaa acggacggcg ctctcctcaa
    29101 cgcaggagag cgcgccaatt tccagccaca cggcggtgac gcgcttaaca tcgtgctgct
    29161 ccgcctgccg ttggataatt tcaacggcgc tctggcaaag cgacaactca tgcattttcg
    29221 cccctcctgc ggccaaacag cagggcgcga cgccctgcgt gtggtacatc cggattggtg
    29281 actggcaacg aaaggatcat tcgtgcgcag tcatccgtca ggcgttggcc ttcttcaatc
    29341 gacatgctgt gcgaaagcgg cgacatcagc gaacaggaga ggtattgcga aacaccgtcc
    29401 agttcgccaa cggtaaaggt catggtgcca tacggcagtt gcagaccaat tttttcactc
    29461 actttgcgca gcggccagag ttgatccggg ccggggaaga taactgcact cagcatccac
    29521 ggggtgatca cacaccccgt ccactgacct tcgaacagcg taaaatcaga aacatacacc
    29581 ggcattgaag gatgcagaaa agagagatcg tgcatcgaac gccgggcaat ttcttcaaac
    29641 gctgcctgta cttgcgcctt cggggaggtc tggaaacctg ctatctcttc agtcatgaat
    29701 cgcctcccgt gggatggctt ccacgcctga ttcgcgcagc gcagccagaa cctgctcaag
    29761 cgcaggttca atcattgctt caaccgtcgg cgttaagccg atgtgtggct ccagcgattc
    29821 cgggatcacg ccgaccaggg tcagcttttt cggaaactca ccggtgaagc gcagggccga
    29881 caatacgtcg gccaggccaa gctgatgcgg agagattttg ttggtaaaca acgccggaac
    29941 ttcttcatcc cgcaggatca tcatcgttcc cggagtgttc tttttcgaca caatggcatc
    30001 cgcaataatc agatgatcgc gatttgccat gtcgccaagc agctccattc ccgccgtgcc
    30061 gccatcgagg atctcaacat aatccggcag aatgtatcgt tgctctaacg cttcgacaat
    30121 ccgcacgccg atggcttcat cggtcagcaa aatattgccg acccctaaga ctaaaatacg
    30181 cattacagaa ccttcactga aaccacttcg ttgccgtcgg catccactac gtgtaccgca
    30241 caggccatgc acgggtcgaa ggagtgaatg gtacgcacca cttccagcgg tttattcgga
    30301 tcggcaaccg gtgtacccac cagcgactgc tcgtaaggac cgacgtcatc attgaagtta
    30361 cgcggaccag agttccaggt tgatggaaca accgcctggt agttgctgat gataccgtct
    30421 ttaataacca tccagtgaga gagcataccg cgcggcgctt cgaggaagcc aacacctttg
    30481 aactcacccg ttgccggaat gttcggcttc acaaaggtgg tgtgatcgcc tttgccgata
    30541 ttggtgatca gtgcactgta ttggttttgc aggatatcct gcaattcaca gcagtgaacg
    30601 gtacgaccaa taatacggcc cagcgtggag tgcagctgtg ccacttccag cgtgttgcca
    30661 gtcagtttct gataaatcgc aacgatttca ttcagtttgt tctgggtaga ttcgcgacct
    30721 gccgccagtt tcaccagcat attagccagc ggacccactt ctaccgtttt gccgtagaaa
    30781 gtcggtgatt tcacccagga atatttcccg tcgtcagacc aaccatcata agccggaatg
    30841 gtggtacctt cccacggcgc ctgtggcgct tcgtctttat accaggagtg cttcgcgctt
    30901 tcctgaatgc ctttgatcag gtattcatcg gaatgagaag tgatcggacg atacgaggac
    30961 agatccgcat tctcaatgta gcctcccggg aacaggaagc tgccgttttt gctgtcggtc
    31021 gggaattccg gcacgctcag gtagttcacc gcacctttac cgcgcgtcag ccattccggg
    31081 tagaacgcgg cgataaccgc ggtatcaacc ttataaacct gctcaacaaa gtcgctcagt
    31141 ttgtcgatga aagacttgat gtacatcagg cgctcaaggt tcaacacgcc caacccgtcg
    31201 aggttgattg ggttcgcgac accacccaca gccaggttct gaatgtgcgg cgttttaccg
    31261 cccagcagcg ccacgacgcg gttagcgtca cgctggcact ccagcgcctg caggtagtgc
    31321 gctaccgcaa tcaggttcac ttccggcggc agtttcatcg ccgggtgacc ccagtagcca
    31381 ttggcgaaaa tacccaactg accgctggca accagatctt tgatcttgtt ctgaacttta
    31441 gtgaactctt ccggactgtt cagatgccag gtcgaaacgc ctttcagcat ttcggaggct
    31501 ttggttgggt cagcttgcag tgcagaagtg atgtccaccc agtccagcgc cgaaagctga
    31561 tagaaatgaa cgatatggtc atgcgtggtg tgcgcagcca gaatgatgtt acggatgtat
    31621 tgcgcgttaa ccggaacgtc gatattcagc gcactttctg ccgcacgaac ggaagacagc
    31681 gcgtgagtgg tagtacatac gccacagata cgttgcacaa tcatccatgc atcgcgcgga
    31741 tcgcggtttt tcacgatctc ttccatgccg cgccacatgg taccggaagc ccatgctttc
    31801 gaaacgacgc cattttcgat ttcgcaatcg atgcgtaaat gcccctcaat acgggttacc
    31861 ggatcaatag taattctctg gctcatgctt tgctcgcctc atgacgatta tgatcgtttt
    31921 gttttaaagg aggaagtatc ggcagtagac gaatgagtac gatgtaagcg caaatctcaa
    31981 tagccacaaa accaatagaa atcaacagtt cttcccaggt cgggaagtag gcgtaaccgc
    32041 cgcccgggtt gaatgccacc aacgaatagg tcagacgcca ggttgcacaa cctaacagtg
    32101 cgctcagtgc tgacaggaac agcatacggg aatcattacg cagcttcgcc acacgcagaa
    32161 cgaccagcgg gaagagcatc agcaggactt caatccagaa catcacggag tagaagtcac
    32221 cggcaaacgc taacgacagc ttgtcgcgat agataagctc gccaaagcgc agcccgatga
    32281 aaatcgccag caacacactg atggtgttgg tcagcttgac gaacaggctc ttttcatccg
    32341 gaccgttgcc acgcagaccc gcctgcacca gtgaaccttc aaagatgaca atcgagaagc
    32401 ccatgatgaa cgccgtcagc agcgagaaca gcggcaacat ttcatagctc tgccagagcg
    32461 gatgcacctt gtagcccgcc gagatcatca gcgaccccat tgaagactgg tgcatggtcg
    32521 gcagcagcgc accgagcgcg atgatgaaga acatcacctt gttcagacgt tgtagcgaca
    32581 ccttccagcc cagacgttca aacagtgccg gagcaaactc cagagccatc acgccgatgt
    32641 agatggtcat acagaccgcc gtctcgaaca gtaccgagtt cacgttgaag tgacccggaa
    32701 tgtagaagta cggcaggttc cagtagcgac ccacgtcgat agtgatcgac aagccaccca
    32761 gtgagtaacc aaacagactc gccaacagcg ccggacgcac cagcggatgg tattgcccac
    32821 ggttaaagac gtataccgcc cacgccagcg cccagccgcc acaggcaaag ccggtgccaa
    32881 tcagcaggtc aaacgcgatc cacacgcccc acgggaagcc gccgttcagg tcagagacag
    32941 agcccagacc gaacaccaga cgcttcacaa tcaggagcat acagatgacg attaacggtc
    33001 caaaaatcat gaccggttta ctgatgattt tgccgcccag cggttgtgga tcatgactca
    33061 tgatcgtctc ctccgtcgtg atggtcgttt ttggtgttgc gacgaaccag cacggttaag
    33121 cccgccagca cagccagtgg tagcatcatg cctttataca gggtgtgttg aatattttcg
    33181 gaacgcgcac cggtagaaag atcgtccagt ttcggcaggt cgagattttc ataaggcaca
    33241 cccgtcagta ccagaacctg agtaccgccg ccctctttct cgccgtacag atgcggataa
    33301 tatttcggca ccgtatgcag gtaagtgtcg ccagatttca gcgtctgacg cggatagtgg
    33361 tattcgctgc caggcttcag cgccagacgt tttttcgcct ccgccatcag ctcttcacgc
    33421 gtaccgaaaa tcaccgcgcc cgccgggcac acttctacgc agccaggcag accgccttta
    33481 tcgagacgtt ccacaccttt ctggttgcac agctcgcact tatgcagcgc accaaacggg
    33541 ttgttgtagt cgtacttcgg cacgttgtac ggacaggcga ccatgcagta acggcaaccg
    33601 gtgcacacgt ctttgtcgta atggacaatg ccggttttcg gatctttttt cagtgcagag
    33661 accgggcaca cagagacaca gttcggatcg acgcagtgca tacactgttt cttaatgtac
    33721 gcgtagccgt tctcctcctg gtctttgttg acccctgtgc cgctggtcca cacctgaatg
    33781 atgttattgg tatacggcga cagtttgtcg ttgttcgacc aggtctgttc cccttgcggg
    33841 ttacgttcag ggaagttgat atcctgacac ttggtgacgc aagcctggca gcctacgcac
    33901 aaggtcgagt cgtacaacat ccccagcgat cccggaattg gcgggcggtt ttcagcagcc
    33961 gcatgactga cagacggcag cgcgcccgtc agcaatgccc cgcaggaggc tgctttaata
    34021 aaattacgtc tgttcacggt tattctcccc gtgagtcagc gttatctttc ttttgctgac
    34081 gacccagttc acgcaccgcc atcacgctga caccggcaac cagcccaacc acaccgccga
    34141 gcaaaccaat agcgcctgca gagacgttgc cgccctcttt agcgttaaca tccggtttct
    34201 gtgaacgcgg agtctgattt tcgacgttgg caagctgatg gatgccttta tggaagccga
    34261 tacctttttc gttacagcca tagcacgggt gaccaatcgc caccggccac acaccgccaa
    34321 catcgcagaa ttgcagcgtt gagcagttgc cgtaagtttc tggcccttta cagccgaggt
    34381 ggtacaagca ccagccttcg cggtggcctt catcaccgaa ctctttggca aaacgaccag
    34441 catcgaagtg cgggcgacgt tcgcagtgtt cgtgaatcag acggccatag gcgaaggtcg
    34501 gacggttttt gtcatccagt ttcggcggtt tgccgtaagt gatgatgtgc gcaacggtcg
    34561 cgaggaagtt gtgcgggttc ggcgggcagc ccggaatatt gataacggtt ttgcctggca
    34621 gaacttcttg caagctgact gcgccagttg ggttaactcc agctgcggca acaccgcccc
    34681 acgcggagca ggaaccaata gcgataatgg ctgctgagcc ttccgccgct ttgcggatgt
    34741 gatccacaat cggctcaccg gcaaccatgc aataaatacc gttatctttt aatgggatgg
    34801 aaccatccac cactaacaca tactgccctt tgtacttctc gagagcgtta tgtttgttct
    34861 cttcgacctg atgaccgaag gcggcggaaa gcacttcgtg atattccaga gagatagtct
    34921 ccagcacgag gttttctacc gttggatgcg ttgcacgaag cagagattcc gtacaaccgg
    34981 tgcactcctg cgcgccaatc cagataactg gcggacgctg cgggttagta accgattcgg
    35041 ccatctctgc agcggctttg ctacttaacc ccatggtggc ggctaatgct gcacaaagct
    35101 tcatgaaatc acgacggtta atgccgtgag aatggatgag ggtgttatct ccagtcattt
    35161 atagttattc cgttgcgaga acctggcata tattttgcct caatcgcaaa atcaataatg
    35221 cgatcgatgc gccatttacc acacatttat tatggttatc gatatcatga tactgcggcg
    35281 gggcgctaaa acgaagggaa gaagcattag tgtaattaat taaagcaaga taatacgtat
    35341 gtttgatcaa ttttcgaggc gataaccggc cataaaaaag cgcaacgtag aacaggaatt
    35401 attctcacga aagaaatcca ttaccatctc tttatccagt ccatagcggc gcgtcagaat
    35461 acccgcttcc caggcgctaa gctggcgatc tccggtcttt tcgtacatcc tgtgtgagta
    35521 gcctaataca aaaccgcgtt tataatcggc acagaatttt gtcacattaa ccgcactgtc
    35581 ggcatgcgtt gcttttaatc ccgccattaa gcctttacca aaatggttat tcatcgttca
    35641 cctcattcat tatatatcgc catatataac gatattttag gcaaagaacg acttttgcgc
    35701 acgctatttt ttcatacttg tttaagacgt aatcccgtaa gagataaacc tatcaggaga
    35761 gatatttatc gaaccatttc agtgtccttt gccaggcaag atcggcggca gatttgtcat
    35821 aacggggcgt ggaatcatta tggaatccgt gattaacccc cggatagata taagcctcat
    35881 aaaccttatt attgtctttc aacgccgcct cgtaaggagg ccagccctcg ttgattcggg
    35941 tgtccagttc cgcgaagtgg agtagtaaag gcgcttcaat cttcgccaca tcggcagtgg
    36001 gtgcctgacg accataaaac ggcaccgcgc aggccagttc cggatacgcg acagccgccg
    36061 cgttcgatac gccaccgcca tagcaaaatc cggtaatacc cactttacct gtcgcttgcg
    36121 gatagcgttg cataaactca attgcggcaa agaaatcatt catcagtttg gttggatcaa
    36181 cctgctgttg cagctcacga cctttatcat catttcccgg ataacctcca acggaactta
    36241 atccgtcagg tgccagggcg atataccccg ccttcgccac tcgccgtgcc acatcttcga
    36301 tatacggatt cagtccacga ttctcatgca ccacccccac ggctggcgtt ttgccgctca
    36361 tttttgcggg tttcaccaga taaccccgta cctcgccgtg accatttggc gaaggatacg
    36421 tgatgtactc agcaacaatc tccgggtcgg taaactctac ctgagtcgcc agcgcataat
    36481 ttggcttgag caaatcaaac aacgctaatg ccgtcatccc gcccaccgca tacttcgccg
    36541 caaggttgag gaactcacgt ttcgaaattt tcccgtgaac gtaatagtcg tagtaatcca
    36601 acaactcttg tgggaaatct ttggcggtta aacgcggcat cgttgcattc ctcaactggt
    36661 gtttttttaa gcaaagcata agcgcgtgtt tttgcccagt ttttcgtcac tctgtgagcc
    36721 agactacggg atacgcgctg gcgaatcgct aaactagaaa cattgtttcg aaattgaacg
    36781 gtggaaagga gaggtcatgg tctggttagc gaatcccgaa cgttacgggc agatgcagta
    36841 ccgctattgc ggaaaaagtg gtttacgcct gcccgcatta tcgctcggtt tatggcacaa
    36901 tttcggtcac gttaacgcgc tggaatcaca gcgtgcaatc ctgcgtaaag cgtttgattt
    36961 aggcattacg cactttgatt tagccaacaa ttacgggccg cctccaggaa gcgcagaaga
    37021 gaactttggt cgcctgctgc gcgaggattt tgctgcttat cgcgatgaac tgattatctc
    37081 taccaaggct ggctacgata tgtggcccgg cccgtacggc tctggcggtt cacgtaaata
    37141 cctgctcgcc agcctcgacc aaagcctgaa gcgtatgggg cttgagtatg tcgatatctt
    37201 ttactctcat cgcgtcgatg aaaatacgcc gatggaagaa accgcctctg cgctggctca
    37261 tgcggtacaa agcggtaagg cgctgtatgt cgggatctcc tcttactcgc cagagcggac
    37321 gcaaaaaatg gtcgagttgc tgcacgagtg gaaaattccg cttttaattc atcaaccttc
    37381 gtacaattta ctgaaccgct gggtggataa aagcggcctg ctggataccc tgcaaaataa
    37441 cggcgtgggc tgtattgcct ttactcctct ggctcaggga ttgctgaccg gaaaatatct
    37501 caacggcatt ccgcaagatt cacggatgca tcgtgaaggg aataaagttc gtggtctgac
    37561 gccgaaaatg cttaccgaag ccaacctcaa cagcctgcgc ttattgaatg aaatggcaca
    37621 gcagcgtgga caatcaatgg cgcaaatggc gttaagctgg ttgctgaaag atgatcgcgt
    37681 gacgtcggta ttgattggtg ccagccgcgc ggagcaacta gaggagaacg tgcaggcgct
    37741 gaataatctg acatttagca ccgaggagct ggcgcagatt gatcagcata tcgccgatgg
    37801 cgagctgaat ctgtggcagg cgtcgtccga taaatgacct gttaataacg ggcgcgcgaa
    37861 ccgcgcccga atcagatcag tgattatgac gagtcagtcg gtcaagatag cccatcacaa
    37921 atgcagaaag cacaaacgtc agatggataa tgacgtacca catcagtttg ttatccggga
    37981 catttttcgc atccataaag acgcgcagta agtgaatgga agaaattgcc acaatcgacg
    38041 ctgctacttt gtttttcagc gacgttgcgt ccattttccc cagccacttc agcttctctt
    38101 tgttctcgga gatatccagc tgtgagacga aattctcata accggaaaac atcaccatca
    38161 ccagcaaacc gccaaccagc gtcatatcca ccagcgacag caacacgagg atcaaatctg
    38221 attccgccat cgagaagata ttcggcagta cgtgaataat ctcctggaag aacttcagcg
    38281 ccagggcaac taacgccagc gaaaggccaa agtacacggg ggcaagcagc cagcgagaag
    38341 catacattgc attttcaaga aaacgttcca tacattcctg tcgtgtagtt aaaacgggca
    38401 acagtatatc gcaattgagt aaacagcacg caacagcaaa tcaacatata cgttaaacct
    38461 ttgtcgcata attctgaggt ttagggatct tcctggttcc cttatcaacg catcgagagc
    38521 gtctaccctt aaaggacgtt gaacctgaag ggagaaaaac gatgtctcat ttaaaagacc
    38581 cgaccacgca gtattacact ggtgaatatc ccaaacagaa acaaccgacg ccaggcatcc
    38641 aggcgaagat gacaccgata ccggattgcg gcgagaaaac ctatgttggt agcggtcgcc
    38701 tgaaagatcg taaagcactg gtgacagggg gcgattccgg aataggtcgc gctgccgcca
    38761 tcgcttacgc gcgtgaaggg gctgacgtgg cgatcagtta tcttcccgtg gaagaagaag
    38821 acgctcagga tgtgaaaaag atcattgaag aatgcggacg caaagccgtt ctgctgccag
    38881 gcgatttaag cgatgagaaa ttcgcccgtt cgctggttca cgaagcgcac aaggcgttag
    38941 gcgggctgga tattatggcg ctggtcgccg ggaaacaggt tgccattccg gatattgcag
    39001 acctcaccag cgaacagttt caaaagacct ttgccattaa cgttttcgcg ctgttctggc
    39061 taacccagga agcgatcccc ctgctaccga aaggtgcaag tgtcatcacc acttcgtcaa
    39121 tccaggcata ccagccaagt ccgcatttac tggactatgc ggctacgaag gcggcgattc
    39181 tgaactacag ccgtggcctg gcaaaacagg tcgcggagaa aggtattcgg gtgaatattg
    39241 tcgcgccagg ccctatctgg acggcactgc aaatttccgg cggacaaacg caggataaga
    39301 tcccgcagtt tggtcagaaa acgccgatga agcgtgcggg gcaaccggcg gaactggccc
    39361 ctgtatatgt ttatctggca agtcaggagt cgagctacgt caccgcagaa gtgcacggcg
    39421 tgtgcggcgg cgagcattta ggttaaaaaa atgcccggtt gtgaaaagca accgggcatc
    39481 attgtgaatt cactcttacc tgatgtggcg aaaacgcctt atccgcctac aaacgcatga
    39541 agattcaaca tgttgcaata gattttgtag gcctgataag cgtagcgcat caggcattga
    39601 gcacctaatg ccggatgcgt cgtgaacgcc ttatccggcc tacaaacgca cgaagattca
    39661 acatgttgca atagattttg taggcctgat gagcgtagcg catcaggcaa ttctacttta
    39721 cttcgctttg gcggtttctt cgccgaccag acctatcttc aggtaacccg cctgatgcag
    39781 cgtatccatt accttcatca acgtctcgta atcgacggtt ttgtccgctc ggaagaagat
    39841 ggtggtgtct ttcttgcctt cggttaacgc attcaacgcc gtaatcattg tttcatcggt
    39901 gaccggatcg ttaccgataa acatcgagtt gtctgccttc accgacagat aaaccggttt
    39961 ttccgggcgc ggctgcggcg tgctggtaga agcaggcaag ttcaccttca catctaccgt
    40021 cgctaacggt gccgccacca taaagataat cagcagaacc aacatcacgt cgataaacgg
    40081 cgtcacgttg atatcatgca tttcgccgtt atcgtcgagg ttttcgttaa gatgcattgc
    40141 catcggacat tatcctgcgc gtaatttttg tgcgacacga accggatgcg cagcggcgct
    40201 ggcttccaga tctaggtcac ggctttgcag caacaatacc tgcgctgcaa catcacccag
    40261 catcgcttta aagccgccaa tctggcgtgc aaatacgtta tagataacga ccgcaggaat
    40321 cgctgccacg aggccgattg ccgttgctaa cagagcttct gcgatacccg gcgcaacgac
    40381 tgccaggtta gtggtctgcg tttgcgcgat accaataaag ctgttcatga tgccccagac
    40441 cgtaccaaac agaccaacga acggagaaat cgcgccgatg gttgccagat agccgttacc
    40501 gcggcccatt tgacgaccca ctgcggcgac ccgacgctcc agacggaagc tggtacgttc
    40561 tttaatacct tcgttatcgt cgctgccttc tgacagttcc agctcattct gcgcttcatt
    40621 gagcaaatgc aggcttaagc ttttgctacc aaaatcagcg gcgatatcgt tggcctggtt
    40681 taaggaacgt gcttcagcca gcagttgctg ctcgcgctta aggcgacgct tctgattgaa
    40741 gaactctacg ctcttactga agaagattgc ccaggtgact acagaggcca aaataagccc
    40801 aatcatcacg cacttaacga caatatcggc gtactgatac ataccccaga cggaaaggtc
    40861 cgtctgcatt aaattattac ccactgtgta tctccaggac gcaagtcaca aaatctgcgc
    40921 ataataatat caaaacgacg tcgaattgat agtcgttctc attactattt gcatactgcc
    40981 gtacctttgc tttcttttcc ttgcgtttac gcagtaaaaa agtcaccagc acgccatttg
    41041 cgaaaatttt ctgctttatg ccaattcttc aggatgcgcc cgcgaatatt catgctagtt
    41101 tagacatcca gacgtataaa aacaggaatc ccgacatggc ggacaaaaag cttgatactc
    41161 aactggtgaa tgcaggacgc agcaaaaaat acactcttgg cgcggtaaat agcgtgattc
    41221 agcgcgcttc ttcgctggta tttgagagta tggaagccaa aaagcacgcg acgcgcaatc
    41281 gcgccaatgg cgagttgttc tatggacggc gcggaacgtt aacccatttt tccttacaac
    41341 aagcgatgtg tgaactggaa ggtggcgcag gctgcgcgct atttccctgc ggggcggcag
    41401 cggttgctaa ttcgattctt gcttttgtcg aacagggcga tcatgtgttg atgaccaaca
    41461 ccgcctatga accgagtcag gatttctgta gcaaaatcct cagcaaactg ggcgtaacga
    41521 catcgtggtt tgatccgctg attggtgccg atatcgttaa gcatctgcag ccaaacacta
    41581 aaatcgtgtt tctggaatcg ccgggttcca tcactatgga agtccacgac gttccggcga
    41641 ttgttgccgc cgtgcgcagt gtggtgccgg atgccatcat tatgatcgac aacacctggg
    41701 cagccggtgt gctgtttaag gcgctggatt ttggcatcga tgtttctatt caggccgcca
    41761 ccaaatatct ggttgggcat tcagatgcga tgattggcac tgccgtgtgc aatgcccgtt
    41821 gctgggagca gctacgggaa aatgcctatc tgatgggcca gatggtcgat gccgataccg
    41881 cctatataac cagccgtggc ctgcgcacat taggtgtgcg tttgcgtcaa catcatgaaa
    41941 gcagtctgaa agtggccgaa tggctggcag aacatccgca agttgcgcga gttaaccacc
    42001 ctgccctgcc tggcagtaaa ggacacgaat tctggaaacg agactttaca ggcagcagcg
    42061 ggctattttc ctttgtgctt aagaaaaaac tcaatgatga agagctggcg aactatctgg
    42121 ataacttcag tttattcagc atggcctact cgtggggcgg gtatgaatcg ttgatcctgg
    42181 caaatcaacc agaacatatc gccgccattc gcccacaagg cgagatcgat tttagcggga
    42241 ccttgattcg cctgcatatt ggtctggaag atgtcgacga tctgattgcc gatctggacg
    42301 ccggttttgc acggattgta taacattgcc acttttggac aattttgcag acattttatt
    42361 gtgaaaagtc ttaaattgtt gcgtccggga tcaaggcgtc ccggacgatt caggagtaca
    42421 ataggcagat aaaggcttaa acgctgttcc acaggaaagt ccatggctgt tattcaagat
    42481 atcatcgctg cgctctggca acacgacttt gccgcgctgg cggatcctca tattgttagc
    42541 gttgtttact ttgtcatgtt tgccacgctg tttttagaaa acggcctgct gcccgcctca
    42601 tttttgccag gcgacagctt gttgatactg gcaggcgcat tgattgccca aggggttatg
    42661 gattttctgc ctacgattgc gattctgacc gccgcagcaa gtctgggctg ctggctaagt
    42721 tatattcagg ggagctggtt agggaatacc aaaacggtga aaggctggct ggcacagctt
    42781 cctgctaaat atcaccagcg cgccacctgc atgtttgacc gccacggtct gctggcgctg
    42841 ctggctggac gttttcttgc atttgtccgt acgctgctgc caaccatggc gggaatttcc
    42901 ggtctgccaa accgccgctt ccagtttttc aactggttaa gtggattgct gtgggtcagc
    42961 gtggtaacca gttttggcta tgccttaagt atgattccgt tcgttaaacg ccatgaagat
    43021 caggtaatga cgttcctgat gatcctgcca attgccttgt taaccgctgg cttgttaggc
    43081 acgctgtttg tggtgattaa aaaaaaatac tgtaacgcct gacgattttc cccgttcccg
    43141 gctgctgtac cgggaacgta tttaattccc ctgcatcgcc cgcattcttg ccgcatcttc
    43201 ccccggcgtc acaccgaagt aacgtttaaa ctcacggcta aattgcgatg cgctttcata
    43261 gccgacgcgc atcgctgctg cgctggcctt catgccgtca tggatgatca tcatccgcgc
    43321 cttatgcaga cggtaattct tcaaatactg caacggcgag gtgctggtga cagacttaaa
    43381 attatggtgg aacgccgata cgctcatgtt ggcttctgcc gccagttgct cgacgctcag
    43441 gttttcggtg tatttattct caattcgttt cagcacgcgg ctaatcagac tgaagtgagt
    43501 ctggcgactg accagcgcca gtaacgcgcc gccgcaaggt ccggtcagca cgtagtacag
    43561 aatttcgcgg atgatctgtt tgccgagaat acgcgcatcc agtggtcgct ccatcacgtc
    43621 gagtaaccgc tccgccgcgc ataaaatctc ttctgataac gtggcggagt taatcccgct
    43681 ggctgccatc gacggctgga aatgctcatc ttcgccaatg tccatcaaca gttcctgtaa
    43741 ctgcaaaata tcgacattga gacgcaaccc tgccagcggc acctctgacg tggcataggt
    43801 ttcgcactca aacggcaacg gcaccgtcag cagcaggtat tcattggcat cataacgaaa
    43861 cacgcgttca ttgatataac cgattttatg cccggaaaag agaattatga tgccaggctc
    43921 gtacatcacc ggtgtacgtg cgaaaggcgt ctcgccatac aacaaacgca catcgggcaa
    43981 cagttctgac aaactatttt ctttattttt cagtttatta actttatccg ccagcaagcg
    44041 acaaatctct tcacgtttca tatcgcgtaa tttcttagga ataatgcggc aatttgattg
    44101 tgcgcaattt tgtagcattt ctccagcact ctggagaaat aggcaagaca ttggcagaaa
    44161 tgagcattga gagccagggc gctggcgatc acaatgaaaa acatcaggca gatcgttctc
    44221 tgccctcata ttggcccagc aaagggagca agtaatgaac aactttaatc tgcacacccc
    44281 aacccgcatt ctgtttggta aaggcgcaat cgctggttta cgcgaacaaa ttcctcacga
    44341 tgctcgcgta ttgattacct acggtggcgg cagcgtgaaa aaaaccggcg ttctcgatca
    44401 agttctggat gccctgaaag gcatggacgt gctggaattt ggcggtattg agccaaaccc
    44461 ggcttatgaa acgctgatga acgccgtgaa actggttcgc gaacagaaag tgactttcct
    44521 gctggcggtt ggcggcggtt ctgtactgga cggcaccaaa tttatcgccg cagcggctaa
    44581 ctatccggaa aatatcgatc cgtggcacat tctgcaaacg ggcggtaaag agattaaaag
    44641 cgccatcccg atgggctgtg tgctgacgct gccagcaacc ggttcagaat ccaacgcagg
    44701 cgcggtgatc tcccgtaaaa ccacaggcga caagcaggcg ttccattctg cacatgttca
    44761 gccggtattt gccgtgctcg atccggttta tacctacacc ctgccgccgc gtcaggtggc
    44821 taacggcgta gtggacgcct ttgtacacac cgtggaacag tatgttacca aaccggttga
    44881 tgccaaaatt caggaccgtt tcgcagaagg cattttgctg acgctaatcg aagatggtcc
    44941 gaaagccctg aaagagccag aaaactacga tgtgcgcgcc aacgtcatgt gggcggcgac
    45001 tcaggcgctg aacggtttga ttggcactgg cgtaccgcag gactgggcaa cgcatatgct
    45061 gggccacgaa ctgactgcga tgcacggtct ggatcacgcg caaacactgg ctatcgtcct
    45121 gcctgcactg tggaatgaaa aacgcgagac caagcgcgct aagctgctgc aatatgctga
    45181 acgcgtctgg aacatcactg aaggttccga cgatgagcgt attgacgccg cgattgccgc
    45241 aacccgcaat ttctttgagc aattaggcgt gccgacccac ctctccgact acggtctgga
    45301 cggcagctcc atcccggctt tgctgaaaaa actggaagag cacggcatga cccaactggg
    45361 cgaaaatcat gacattacgt tggatgtcag ccgccgtata tacgaagccg cccgctaagc
    45421 tttttacgcc tcaaactttc gttttcgggc atttcgtcca gacttaagtt cacaacacct
    45481 caccggagcc tgctccggtg agttcatata aaggaggaac gtatggctaa tccaaccgtt
    45541 attaagctac aggatggcaa tgtcatgccc cagctgggac tgggcgtctg gcaagcaagt
    45601 aatgaggaag taatcaccgc cattcaaaaa gcgttagaag tgggttatcg ctcgattgat
    45661 accgccgcgg cctacaagaa cgaagaaggt gtcggcaaag ccctgaaaaa tgcctcagtc
    45721 aacagagaag aactgttcat caccactaag ctgtggaacg acgaccacaa gcgcccccgc
    45781 gaagccctgc tcgacagcct gaaaaaactc cagcttgatt atatcgacct ctacttaatg
    45841 cactggcccg ttcccgctat cgaccattat gtcgaagcat ggaaaggcat gatcgaattg
    45901 caaaaagagg gattaatcaa aagcatcggc gtgtgcaact tccagatcca tcacctgcaa
    45961 cgcctgattg atgaaactgg cgtgacgcct gtgataaacc agatcgaact tcatccgctg
    46021 atgcaacaac gccagctaca cgcttggaac gcgacacaca aaatccagac cgaatcctgg
    46081 agcccattag cgcaaggagg gaaaggcgtt ttcgatcaga aagtcattcg cgatctggca
    46141 gataaatacg gcaaaacccc ggcgcagatt gttatccgct ggcatctgga tagcggcctg
    46201 gtggtgatcc cgaaatcggt cacaccttca cgtattgccg aaaactttga tgtctgggat
    46261 ttccgtctcg acaaagacga actcggcaaa attgcaaaac tcgatcaggg caagcgtctc
    46321 ggtcccgatc ctgaccagtt cggcggctaa cgtgcaaatt ctcccggtgg cggtaatgtt
    46381 ccgctaccgg acttttcaga aatcatttat tcccctcgcg tcccgcccgt tgttactctt
    46441 ccttgttcag gaatgccaaa tataaggaca tcatcatgca gagccggaag ctcttaaaag
    46501 aacaactcat ctatatccgg gataaacgca acggagaggt gaaaaacaga tgaaaataat
    46561 acttctgttt ttagcagccc tggcaagttt taccgtacac gcgcagcctc cttcacagac
    46621 cgtagaacaa acagtccggc agatttatca gaactataaa tcagatgcca gtacgcctta
    46681 tttcggtgaa accggagagc gggcgataac ttcagcgcgt attcaacagg cgcttaccct
    46741 gaacgacaat cttacactac cgggcaatat tggctggctg gattatgatc cggtttgtga
    46801 ttgtcaggat tttggcgatc tggtgttaga aagcgtagcg ataacccaaa ctgacgtcga
    46861 tcatgccgat gccgttgtgc gctttcgtat ctttaaagat gataaagaaa agaccacaca
    46921 gacactgaaa atggtggcgg aaaatggtcg ttgggtcatt gatgatattg tcagcaatca
    46981 tggcagcgtc ttacaagcag ttaatagcga gaatgaaaaa acgctggccg ctttagcttc
    47041 gttgcagaaa gaacagccag aatcctttgt tgctgaactc tttgaacata ttgctgatta
    47101 tagctggccg tggacgtggg tggtttccga ctcttaccgc caggcggtta atgccttcta
    47161 taaaaccacc ttcaagacgg ccaataatcc cgatgaagat atgcaaatag aacggcaatt
    47221 tatttacgac aatccgatct gttttggcga agagtcgcta ttttcacgcg ttgatgaaat
    47281 tcgagtcctg gagaaaaccg ccgattccgc ccgcattcat gttcgtttta cgctgaccaa
    47341 tggcaacaat gaagagcaag agctggtttt acagcggcgc gaaggtaagt gggaaatcgc
    47401 tgattttatc cgcccgaaca gcggcagcct acttaagcag attgaggcaa aaaccgccgc
    47461 cagattaaag caatgagctg aattaaataa caattagccg gaacaataaa taaaagggaa
    47521 cactatatga aaacgatttt caccgtggga gctgttgttc tggcaacctg cttgctcagt
    47581 ggctgcgtca atgagcaaaa ggtcaatcaa ctggcgagca atgtacaaac attaaatgcc
    47641 aaaatcgccc ggcttgagca ggatatgaaa gcactacgcc cacaaatcta tgctgccaaa
    47701 tccgaagcta acagagccaa tacgcgtctt gatgcacagg actattttga ttgcctgcgc
    47761 tgcttgcgta tgtacgcaga atgataaaaa aatccccggc agcatgtcag ttgccgggga
    47821 ttttttttaa cgtccaaccg ccgctttagg gcgtttcttc gcaccagcat tcaccggacg
    47881 agattgcgta gacgacgctt tttttgccgt agcaggcgtc tgacgctgag tcgccatcgg
    47941 cgtatgttta gtcaacgccg gacgggtatt gcggttctgg cgacgagctt cacgcatctc
    48001 ttcaatggtt ggcgcaggca ctaagcaatc gcgacggctg ccaatcagat gctttttgcc
    48061 catcgcttcc agcgcctggc ggattaacgg ccagtttgcc ggatcgtggt aacgcaacaa
    48121 cgctttatgc aaacgacgct gtttgtcgcc cttcggtacg aagacgcctt cgctcttata
    48181 accaatcttc gccagcggtt ttttcccggt gtaatacatg gtggttgagt tcgccagcgg
    48241 cgatgggtag aagttctgta cctggtcgag acggaaacga tgctttttca gccacagcgc
    48301 caaattcacc atatcttcat cacgcgtacc ggggtgcgca gagatgaaat acgggatcag
    48361 atactgctct ttacctgcct gtttcgagta ggtatcgaac agctctttaa agcggtcata
    48421 gctgcccatg cccggcttca tcatcttcga caacggccct tcttcggtat gttccggagc
    48481 aatcttcaga taaccgccga cgtgatgggt cgccaactct ttgatatagc gcggatcttc
    48541 cacggcgatg tcataacgta cgcctgaggc gataaggatt tttttaatgc ctttcagatc
    48601 acgcgcacgg cgatagaggt tgatcgtcgg ttcgtggtta gtgtccatgt gcggacaaat
    48661 atccggataa acgcacgaca aacggcgaca agtttgttca gcgcgtggcg atttgcagcg
    48721 caacatatac atgttggcag ttggcccacc gagatcggaa atcacgcccg taaaacctgg
    48781 aacggtgtcg cggatcgctt cgatctcatt aatgatcgaa tcttccgaac ggctctgaat
    48841 aatgcgccct tcgtgctcgg taatagaaca gaaagaacag ccgccaaagc agccgcgcat
    48901 aatgttgacc gaaaaacgga tcatttcgta agccggaata cgggcattgc cataggccgg
    48961 atgtggcacg cgcttgtacg gcagcgcaaa aacgctgtcc atctcttcgg tagaaagcgg
    49021 gatagcaggc gggttgatcc acacatagcg gtcaccgtgt ttttgcatca atgcgcgggc
    49081 gcagcctggg ttggtttcgt ggtgcagaat gcgcgaagca tgggcgtaca gcactttatc
    49141 gcccttcact ttctcgaaag aaggcagcaa tacgtaggtt ttttcccacg gtttctggcg
    49201 cggtggctgc acgattacag ctttcgcttc ctgctttttc ggcgcaacag gtttgttatc
    49261 cgcacacggc aaatcttcgc catacggatg cgggattggg tcgattttcc ctagggtatc
    49321 aagacgggtg gaatccacac cgctccagcc aggcagcgcc tcttttacga taattgcggt
    49381 attacgcaca tcgcggattt cactaatcgg ctcgcccatc gccagacgat gcgctacttc
    49441 caccagcgga cgttcaccgt taccaaacat cagcatgtcg gctttcgaat ctaccagaac
    49501 ggaacggcgc acggtatcgg accagtaatc ataatgcgcg gtacggcgca ggctagcctc
    49561 aataccgccg aggatcaccg gcacatcttt ccacgcctct ttacaacgct gggtataaac
    49621 cagcgtggcg cgatccggtc gcttacccgc gacattatct ggcgtgtagg catcatcatg
    49681 acgtaaacgg cgatcggcgg tatagcggtt gatcatcgaa tccatgttgc cagcagtaac
    49741 accaaaaaac agattcggtt tacccagacg cataaagtcg tctttgctgc tccagtccgg
    49801 ctgggcgatg atcccgacgc gaaagccctg cgcttccagc atacgaccgc aaatcgccat
    49861 cccgaagctt gggtgatcga catacgcgtc gccagtaacc aaaatgatgt cgcagctatc
    49921 ccagccaagt tgatccatct cttcacgaga catcggcaaa aacggtgccg gtccaaaaca
    49981 ggcggcccag tactgcggcc aggagaacag gtcgcgatcc ggttggatca gggagataga
    50041 gctcattttg cttccagaaa tgataaaaaa ataatcaaag gccggggatt ataagccgga
    50101 acgaaagaga aatcgaaagg tattccatac tcgccctcct cgggcgagta tgaagattac
    50161 ggtaccggat tgaccaacag ttgcccaatc gagccacggt ccgccatttc cagcgtctga
    50221 ctgttgaagt agaacgggaa gtgcgcccag gaaggctgac cgaaataaac aagcagctcc
    50281 acttgtccgt caacccaaac ggtatctttc cagcctctgt cttccgggaa cggcatcgcg
    50341 ccattcacgt tacggatctg gaacattacg ccttcaatat ggaacgcttg cggctcgtcc
    50401 gcgcgtaccg tccagcgttc ccacgttcct tgctgcgcgg tgacatcaat acggttgacg
    50461 tcccacagct gtccattaat acccgggtca tcacccagac tgatatcgcg actgcgaatt
    50521 ggcgaaccag ccatgatttc agttggcagc aagcgcatcg gaagactgtc tgtgaccagc
    50581 ggcagaaggc cggttgggcg cagcgttagc accaaggtag aaaccagaat actggatggc
    50641 tcaaagaagc cgcgaatacg atcaacaatg ctcgccgctt caccacaggt gatcgacact
    50701 tcatcgccgt tgctcatatc caccagaatc tcgcggcgct cgcccggtgc cagcgaaagt
    50761 tgcttcaccg atacaggagc aggcaggaat ccctgatcgc cagaaatcac atgtaacggg
    50821 cgaccatcgc tcatctgtag ttgatagcga cgagagttcg acgcgttcag cagtcgcaag
    50881 cgcacccagc cacgcgagac ttcaacgtac gggctttgta caccgttaac cagcagcgta
    50941 tcaccaacaa agccgccgct tcccggttcg ttgtattctg gcgtaccaaa gttatccagc
    51001 cgtttatcct ggataatgac cggaaaatca tccacaccat aatggttggg gataggcagc
    51061 gacttgctga cttcatcttc caccagccac attccggcaa ggccgttata gacctgctga
    51121 gccgtgcggt tgggagtatt ggcgtgatac cacagagtag ctgcgttctg gcgaataggc
    51181 agtacgggtg cccagtcagc gtttggcgac atcatccgtg ccggaccgcc catcagcggg
    51241 cctggtacct gtagcccggc caccgtcatt gagacatttt ctgtcaggcg gttgctgtaa
    51301 ataagcttaa catcgtcgcc cttccagacg cggatagtcg gccccaggta acgaccattg
    51361 attccccaga ccgacgcgcg tgtccctggc gtaaatgacc agtgcgcacg ttgtacagtc
    51421 ataaacagcg gttgcccacg gcgagattca agtagcggcg gaacgggtag cggttgctgt
    51481 tgcccggctg cgctggcctt caggggaaca gcgcctgcac aaagtgcaat ccccgatgcc
    51541 tgaatgaact gacgccgact gagtgacata tttgctccat gtaaaactgg ctaaagtaat
    51601 accgtgcagg aaagtgttcc ccgcaaaaat acagattgcc gacttaaact tttccggcgg
    51661 cttcgcgttc tgcgacttct ttatcgagct cggcgatttt ttgttccatt atcgaacgac
    51721 aatgggcagc cagctcacga acctgatctt tgccatactg actgacgtca attggcggca
    51781 gcatttcgac aatcaccaga ccgttgtgca gtcgattaag attaatctta ttcgaagttg
    51841 tagagacgca cacgggaata atcgggacgc ccgccgcaat tgccgcgtga aatgctccag
    51901 tcttgaacgg tagcaggccg cgaccacggc tgcgggttcc ttccgggaac atccagatgg
    51961 aaatacggcg ttttttgaaa tgattcacga cttccgcaat ggtgccgtga gctttagtgc
    52021 gattgtttct gtcgatcaat aagttgccgg ttaaccagta caactgcccg aagaagggga
    52081 tccacagcaa gctcttttta cctaccgtca ccgtcggcgg ttgcacaatg ttcgatgctg
    52141 tcaccatgtc atagttgttc tggtggttag cgatatagat agcattgccg tagctttcag
    52201 catctgcagg tttgcggcac tcaactttca ggccgaacag tggcgcaaga cggccaaaca
    52261 tatgcccaaa ggtggccaca tgtttcgggt tacgcgggct gaaaaggcag taaatggagc
    52321 cgaatacaca gactaagatg ctgtaaatca cggtaataat aagacgaaag atatatagca
    52381 taacaccctc tgaaggtctg agagactggc attgtacgtc acctgaaatc aggttagggc
    52441 attgttaaaa gccgctctct ggaaaagcgt attgttaatc gcaacgcatg aataaacaac
    52501 ggtttcaagg gaatttttat cgtttccggg ggttgaatac gctgccggat gacgacttaa
    52561 cgtttcatcc ggcgttcctt gctagcggga ggaaacagcg ccctccccgg catattactc
    52621 ttcgctatca ccgctgctgg cacggcgagg agagtcgatc tcaacacgat cgatacgctg
    52681 caaaccgcgc atcaacgtac cgcggcgtcc acgttcgcca gtgactttct gtagctcttc
    52741 cggacgcagt ttaattttgc gtttcccaac atgaatggtc agcgtgcttt gcggcggcag
    52801 aacgtacagt tgcgccagac catcctcgcc acgcgcggct tctgccgatg gaatgttgat
    52861 aatcttgttg cctttgccct tcgacagctg cggcagatcg cttaccggga acatcaacat
    52921 acggcctgcc tgagtgattg ccagcagcat atcggaagca tcttcaatca ccaccggcgg
    52981 cataacatgg gcattttccg gtaaggtgat caaagcctta cccgcacggt tacgcgctac
    53041 cagatcgtta aaggtgcaga cgaaaccgta acccgcatcg gaagccatca gcagtttctg
    53101 atcgtcgctt tccatcagca tatggtcaac ggtcgcccca ggcggcaacg ttaatttgcc
    53161 ggtgagtggt tcgccctgac cacgcgccga cggcagcgta atcgggtcga tggcatagct
    53221 acgaccggtg gaatcaacaa acactaccgg ttggttactc ttacctttca ccgccgcttt
    53281 gaagctatca cccgctttat aattcaggcc cggcgcgtcg atatcatggc ctttagcgct
    53341 gcgtacccag cccatctgcg acagcacaat ggtgacaggt tcagacggca gcatgtcgtg
    53401 ctcgctcatc gctttcgctt cttcgcgttc ctgcaacggc gaacgacgat cgtcaccgta
    53461 ggcttgcgcg tctgcctgca gttctttctt cagcaggtta ttcattttac gctcggaagc
    53521 caaaatgccc tgcaactggt cgcgctcttt ttccagctca ctctgctcac cgcgaatctt
    53581 catctcttcc agtttggcaa gatgacgcag tttcagttcg aggatcgctt ccgcctgggt
    53641 ttccgtaagg ccaaaccgcg acatcagcgc cggtttcggt tcatcttcgt tacgaatgat
    53701 ctcaatcact tcgtcgatgt tgagaaacgc caccagcaaa ccttccagga tatgcaggcg
    53761 cttgaggact ttctccagac gatagttcag tcggcggcgc acggtatcgc gacggaacac
    53821 cagccattcg gagaggattt ccagcaggtt tttcaccgcc ggacgaccat ccagaccgat
    53881 catattgagg ttaatgcgat agctcttttc cagatcggtg gtagcgaaga ggtggttcat
    53941 cacctgatcc atatccacgc ggttggaacg cggcacaatc accagacggg tcgggttctc
    54001 gtggtcagat tcatcgcgca gatcgtcaac catcggcagc tttttgttgc gcatttgcgc
    54061 agcaatttgc tccagtacgc gcgcacctga aacctgatgc ggtaatgcgc tgataaccac
    54121 cgcgccatct tctttcttcc acaccgcgcg catacgcact gaaccacgtc cgttctcgta
    54181 gattttacga atctcggcgc gcgaagtgat aatttccgct tcagtcggat aatccggccc
    54241 ctgcacgata tccagcagct gatcgagcgt agttttcggc tggtcgatta atgcgattgc
    54301 cgcctgagct acttcacgca ggttatgcgg tggaatatcg gtcgccatgc cgacggcaat
    54361 accggtggtg ccgttaagca aaatgtttgg cagacgggca ggtagcattt tcggctcctg
    54421 caaagtgccg tcgaagtttg gcacccagtc agccgtcccc tgccccagct cgctcaatag
    54481 cagctcggaa tatttcgaca accgggattc ggtgtaacgc attgccgcga acgatttcgg
    54541 atcgtccggc gcgccccagt tcccctgacc atcaaccagc ggataacggt aagagaacgg
    54601 ctgcgccatc aggaccatcg cttcataaca ggcgatatcg ccgtgcggat ggtatttacc
    54661 cagtacgtca ccgacggtac gggccgattt tttaaatttg gcgctggcat tcaggcccag
    54721 ttcagacatc gcatacacaa tgcggcgctg aacaggtttc agaccatcac caataaacgg
    54781 caacgcacgg tccatgatca cgtacatgga gtagtttaag taggcgtttt ccgtaaattc
    54841 atgtagcgca aggcgctctg ccatatcgct cattaattct gattcctcaa cttattcgcc
    54901 agccttcaaa ggggcaatat tgccggagat actacattat ctgccgcgtc gagtcacagg
    54961 agatcagcgt gaatagcgcc gccggacgtg ctccgacggc gataaaggca tcaatgtgcc
    55021 ttgatataca actctttgct gtagacttga tcggcataga gggcagcaca aggttgcgcc
    55081 catgccacac cactcggcat gggggtccgc tccgggcgat tcgagaagtt gaggttatgc
    55141 gagtccaggg aagcctaaag catcaaagta gtcgactgta agtatacggt tcagcccagt
    55201 ttagctgtgt ctccaccagt gccccgcgcc tcctgccata agtgcggctt gctcatggat
    55261 atccccgaga cggcttacgg cgcaccggat aataaatttc gcgtatacat ggtattcctt
    55321 accggagaaa ttataagccc aacaacaata cgcctgtgac attgttcgct caaacttatc
    55381 gcgagtgatt tggctcacac tccggtaaag attccccatc acgccaacgg cacataaaca
    55441 tccgtttcca gtcgctgctc tgtcacccct tcggcaagat tggtgtagtg aaacagaatc
    55501 ggtgctttac gcattttctc gccgcttgca ggcagccagt ggcgaataat gccccatatc
    55561 gtgtgtgaaa tatcgtccag ctcgccaacg tggcgggcca cggcataacg tccaccggta
    55621 agttcaccat tgctcacacc ataacgatta tcgggaatcg gttcgctaac gctgccgcag
    55681 atatcaaagc gaaacgcttc cggtgcggtg gtggcagggg cgtcccaggc gacgccaaaa
    55741 gtctggcttt ggttaacagg agaaagcccc gtctctttac gccacatgat aaatttcgcc
    55801 acactgtagt tgagcaactc agggctacag cggtgcggca acatcgccac agggattgat
    55861 gggaaatcga taatgtttac atccagtgtc aggtttgtca ttttgcctcc ctgcgaaatt
    55921 attcccggga acgcgtcccg ggagcggtaa caattacgga tttactttgc ggatctgttt
    55981 gacgtcaatt tcaacagaat tccagtcttt atcgacttca ccctgaatct caaccgtatc
    56041 tttcggcgtc accgtcacac cgttccagcg tttgtgatcg atatcaacat tgatagtacc
    56101 gctggcatct ttaaacacgt agagatcgtc agagatgcgt tcaacgatat tgccgcgcag
    56161 ggttacccag gtgtcgtcac gcagggattt tgcgctttct acagtcgtta cgctgccgtt
    56221 cggcccctga aatcctccgg cctgactttg cgttaccgat gggccagaaa aaccgccctg
    56281 ctctgctgcc atcaccggtg cactgcacag ggccattact gcgattactg ctgcgaattt
    56341 tttcatgttt attactccct ttaatgtctg tttccgagca tttaacaaga tagtccttaa
    56401 caacttctta agggaaaaaa ataaaattta atgctgtaca gagcgcgtta caacacggtt
    56461 tactggcagc aaatacggtt atcgcaggga tgaaaaaatg cgaattttac tgatagaaga
    56521 tgacatgctg attggtgacg gcatcaaaac tggccttagt aaaatgggtt ttagtgtcga
    56581 ctggtttaca caaggtcgtc agggaaaaga ggcgctttat agcgcacctt atgatgcggt
    56641 gatcctggat ttaaccttac caggcatgga tggtcgcgat attttgcgcg aatggcgaga
    56701 gaaaggtcag cgtgagccgg tactgaacct gaccgcgcgt gatgcgttag cggaacgtgt
    56761 agaagggctg cgtctgggag ctgacgatta tctgtgtaaa ccttttgcgt tgatagaagt
    56821 cgccgccagg ctggaggctc tgatgcgccg aaccaacggc caggccagca acgagctgcg
    56881 ccacggtaac gtcatgctcg accccggcaa acgtatcgcc acgctggctg gcgaaccctt
    56941 aacactgaaa ccaaaagaat ttgccctgct ggaattactg atgcgtaacg ctggtcgggt
    57001 actgccgcgc aaactgattg aagagaaact gtatacctgg gacgaagagg tcaccagtaa
    57061 tgccgttgaa gtgcatgtgc atcatctgcg acgcaaactc ggtagtgatt ttattcgtac
    57121 cgtgcatggt attggctaca cattaggtga gaaatgaaat ttacccaacg tcttagtctg
    57181 cgcgtcaggc tgacgctaat ctttttaatt ctggcctcgg tgacctggct gctttccagc
    57241 tttgtcgcct ggaaacaaac aacggataac gtcgatgaat tgttcgacac ccaactgatg
    57301 ctgtttgcca aacggttaag tacgctcgat ctcaacgaaa tcaacgcggc ggatcgcatg
    57361 gcacagacgc caaataaatt aaaacacggt catgttgatg acgatgcgct gacctttgcc
    57421 atctttaccc acgacggcag aatggtcctt aatgatggcg ataacggaga agatattccc
    57481 tatagctatc aacgggaagg ttttgctgac gggcaactgg tcggtgaaga cgatccttgg
    57541 cgttttgtct ggatgacctc acctgatggc aaatatcgca tcgttgttgg ccaggaatgg
    57601 gaataccgtg aagacatggc gctggcgatt gttgccgggc aattgatccc gtggctggtc
    57661 gcactgccga ttatgttaat catcatgatg gtactactgg gtcgtgaact cgcgccgctg
    57721 aacaaactgg cgctggcact acgtatgcgt gaccctgact cggaaaaacc actaaacgcg
    57781 actggcgtac ccagcgaagt gcggccactg gttgagtcgc taaatcaact gttcgcccgc
    57841 acacatgcga tgatggttcg agaacgacgc tttacctccg atgcagctca cgaactacgt
    57901 agcccgttaa cagcgctgaa agtgcaaacc gaagttgcgc agctctctga cgatgatccg
    57961 caggcgcgga aaaaagcact gctccaatta cattccggga tcgatcgcgc tactcgtctg
    58021 gttgatcaac tgctcacgct atcgcggctg gactcactgg ataaccttca ggacgtcgcg
    58081 gagatcccgc ttgaagatct cctacaatcg tcggtgatgg atatttacca cacggcgcag
    58141 caggcgaaaa ttgacgtgcg actgacactc aatgcccacg gcatcaaacg caccggacaa
    58201 ccgctattgc taagtttgtt ggtgcgaaat ttgctggata acggccccgg tgtgacacca
    58261 gaggcactgg cgcgaattgg cgaacgcttc tatcgcccac ccggacaaac cgctaccggc
    58321 agtggacttg ggctatcgat tgtccagcga atcgccaagc tgcatgacat gaatgttgag
    58381 tttgggaatg cggaacaagg tggatttgag gcgaaggtaa gctggtaact tctctgtcta
    58441 tgagagccgt taaaacgact ctcatagatt ttactaatag caaaatataa accgtcccca
    58501 aaaaagccac caaccacaaa ccagacaaat aacgagcgag aatacatata gccaaaactc
    58561 gaacatttcc cgcataaaga gttttcttaa gataagaata ataagtggcg taagaagaaa
    58621 aaatgctgca atcattgctt gcaatatacg atcttcgcct gaaacggaag gtatactgca
    58681 aatattgcct cccgactcaa gcatccagtc atacttattg aaagcaaaaa aagaatctat
    58741 taaccaataa gtaagcatta tatatggcaa tgcttcaaat attgttttaa ttttctgctt
    58801 taacatatat cagtacgctc ataaaaatcc atttataatt atcaacatga cacgttgata
    58861 ttttcatctt ttacttattc aaagccatat tttcaatccc caaaaaaacc gaaccaaacg
    58921 tgtaaattat tccggtgcga gtaagaatta tgaggaatgg ctatcagtat tgtcattttc
    58981 agaaaatatt tatcctgcat cggtgagtca gagctagatc agacttttgc taaattcgca
    59041 aaagactttg cacattttgc taatttcaca gtaccgctct gtgacgtact atagtcgaca
    59101 aacgtctcac cttgagatta aaaaatgagc aacatcctga ttatcaacgg cgcgaaaaaa
    59161 ttcgcccact ccaatggtca actgaacgac accctgaccg aagtcgcgga tggcacactg
    59221 cgcgacctcg ggcatgatgt ccgcatcgtt cgcgccgaca gcgactacaa tgtcaaagcg
    59281 gaagtacaaa actttctctg ggctgatgtg gtgatctggc agatgccagg ctggtggatg
    59341 ggcgcgccgt ggacagtgaa aaaatatatt gatgatgtat tcaccgaagg tcacgggact
    59401 ctgtatgcca gcgatggtcg tacccgcaaa gatccgtcga aaaaatacgg ttccggcagc
    59461 ctggtacagg acaaaaaata tatgctttct ctaacctgga acgcaccaat ggaagccttc
    59521 accgaaaaag atcagttctt ccacggcgtt ggcgttgacg gtgtgtatct gccgttccat
    59581 aaagcaaacc aattcctcgg tatggaaccg ctgccgacat ttatcgctaa tgacgtgata
    59641 aaaatgcctg atgttccccg ctatactgaa gaatatcgca agcatcttgt ggaaattttt
    59701 ggttaactag agctcaggct ttagaaggag ttaaccatgc ttaccgtaat cgcagaaatc
    59761 cgtactcgtc ctggtcaaca tcaccgtcag gcggtattgg atcagtttgc taaaatcgtt
    59821 ccaaccgtac tgaaagaaga aggttgccac ggctatgcgc caatggtgga ttgcgctgct
    59881 ggcgtgagtt tccagtctat ggcaccggat tctatcgtga tgattgagca gtgggaaagc
    59941 atcgcgcacc ttgaagcgca tctgcaaacc ccgcacatga aggcgtacag cgaagccgta
    60001 aaaggcgacg tgctggagat gaatatccgt attctgcagc cagggattta atcctgcctt
    60061 gtttgcccgg ccatcctgac cgggcaatgt tctttccttt aaacctcaat ctccgccatg
    60121 tcgcctttct cttgcaacca gttgcggcga tcttccgagc gtttcttcgc cagcagcata
    60181 tccatcatcg cgtcagtacg ctgatcgtct tcatcatcga tagtcaactg caccagacgg
    60241 cgagtgttcg gatcaagcgt ggtttcacgc aattgcatcg ggttcatttc ccccagacct
    60301 ttaaaacgct ggacgttcgg cttgcctttc ttgcgtttta attgctcaag tacgccctct
    60361 ttctcttctt ccgtcagcgc gtaataaacc tctttcccga gatcaatacg gtagagcggt
    60421 ggcagtgcga cgtaaacgtg accgtgtttc accaacgcgc ggaagtgttt tacgaacaaa
    60481 gcgcagagca gcgtggcaat gtgcagacca tcagagtccg catccgcgag gatacagatt
    60541 ttgccataac gaagctggct cagatcgtcg ctgtcaggat cgataccgat cgctaccgaa
    60601 atatcatgca cttcctgcga ggccagcact tcgtcggaag aaacttccca ggtgttaagg
    60661 atcttacctt tcagcggcat gatcgcctga tattcacgat cgcgcgcctg cttggcagat
    60721 ccgcctgcgg agtcaccttc cacgaggaac agttcggtac ggttaaggcc ctgcgcggta
    60781 caatcagcca gtttgccagg cagcgccggg ccgctggtca gctttttacg caccactttt
    60841 ttagccgcac gcatacgacg ctgggcgctg gaaatcgcca tctctgccag caactccgcc
    60901 gcctgaacgt tctggttcag ccacaggatg aaggcatctt tcaccacgcc cgaaacgaat
    60961 gccgcacact gacgcgaaga gagacgctct ttggtctgcc cggcaaactg cggatcctgc
    61021 attttaaccg acagaacata ggcgcagcga tcccagatat cttccgccga cagcttcacg
    61081 ccgcgcggca gaatgttgcg gtattcacag aactcacgca tcgcgtccaa caggccctga
    61141 cgcagaccat taacatgggt accgccctgc atcgttggga taaggttgac gtagctttcg
    61201 gtcagcagtt caccgccttc cggcagccac agtagcgccc agtccacagc ttcagtatcg
    61261 ccagcgaaat taccgataaa cggtttttct ggcagcgtcg gtaaaccgtt taccgcttcc
    61321 gccaggtaat cattcagacc gtcctgatag caccagcgct gttcggtgtt gttgatctca
    61381 tctttaaagg taatctcaac gccggggcac agtactgctt tggccttcag cacatgcgtc
    61441 aggcgtgaaa cagaaaaacg cgggctgtca aagaaggttt catccggcca gaagtgcacg
    61501 ctggtaccgg tattgcgttt accgcaagtg ccgacaacct gtaaatcctg caccttttcg
    61561 ccattttcaa aggcgatgtt ataaacctga ccatcgcggc gcacgttaac ttctacgcgc
    61621 ttcgacaagg cgttaactac cgaaatcccc acgccatgca ggccgccaga gaactggtaa
    61681 tttttgttag agaatttacc gcctgcatgc agacggcaaa gaatcagttc aaccgccggt
    61741 actccttctt ccgggtgaat atccaccggc atcccgcgcc catcgtcaat cacttctaac
    61801 gactggtcag catgtaagat tacatccacg cgttttgcgt gacccgccag tgcttcatcg
    61861 acactgttat caataacttc ttgccccaaa tggttagggc gagtggtatc ggtatacatc
    61921 cccggacggc ggcgaaccgg ctcaagcccg gtgagtacct caatggcatc agcgttataa
    61981 gtttgcgtca tggtttaagt tagcaattcg agttgatcgt cagagatggt gcagaccaag
    62041 aaaatcgacg atcgggttga aataatcttc gaagcccgtg aatgcgtggt tgccgccttc
    62101 tatgacagtc tggcggcagg aagcatagta cgccaccgcc tggcggtaat ccagcacttc
    62161 atctcccgtc tgttgcagca gccagatcaa atccggcgct tccagcgggt caatctgcat
    62221 gactttgaga tcgtaaatat ggcgtgactc tagcacatat tgctgcccgg tgtaggggtt
    62281 ctcgttctga ccgagatagt ccgtcagcaa ttcaaacggg cgtaccgccg ggtttaccac
    62341 cactgcgggc agcataaaac attgcgacaa ccaggtggcg taatatcccc ccagtgacga
    62401 accgacaata cccagcgaat cgccgccatg ttccaggaca atggattcca gcagttctgc
    62461 cgcgtcggaa ggatacggcg gcaactgcgg aatgatcatc tcaacgtcag ggtgatgttc
    62521 cgccagccag ttttttaaca agctcgcttt tgcagagcgc ggcgagctgt tgaaaccgtg
    62581 taaataaaga agcgtagaca tcagtagcct tctgaagcgg tatcaggttg gaaacgtgtg
    62641 tccgccaggc gatgcacctc ggtggtcagc gtgccatcag catgtaactc gagagtacgc
    62701 cagccgggcg cgatagtatc cagcgtaaag ttggaacagt gcggcttaaa ctgcacacag
    62761 gtcgacggcg ttgccagcag gcggcgaccg ttccagtcga gatccagctc ctgatgaata
    62821 tgaccgcaca gcaagtattt gacgtgcgga aacttcgcca gcacggtatc cagttcgccc
    62881 gcgttacgca gactgtgttg atcgagccaa ctacaacccg caggtagcgg atgatgatgc
    62941 agcagcagca acgtatggcg ttctggcgca tcggccagtt tacgttccag ccactcaagc
    63001 tgaaactcgc tcagctcacc gtgcggcacg ccaaacacct ggctatccag caacaggatt
    63061 tgccattgct caccaataaa cacccgcttc gccggggaga tacccgcatc ctgtaacgcg
    63121 ctgtacatcg cgggctggaa atcgtggttg cccggcagcc agacgcaggg cgcacgaaaa
    63181 cttgcgatgc cttcagcgaa atgctgatag gccgcagagg attgatcctg cgctaaatca
    63241 cctgtcgcga caatcaggtc gaattcgtgc tggtgtggac gaatcgcctc cagcaccgcc
    63301 tggtagcttt cccaggtgtt tacccctaac agggcttcgt gcttttgtgc aaacaggtga
    63361 gtatcggtaa tttgtaaaat ccttcctctg gcctcaccag ccagaggaag ggttaacagg
    63421 ctttccaaat ggtgtcctta ggtttcacga cgctagtaaa ccggaatcgc catcgctcca
    63481 tgtgctaaac agtatcgcaa ccagtccgct aaaaactggt taatttgatg cttttcgtcg
    63541 cgttgatgca actttttatt aggataatca taccgcgctt tgaagcgaaa aatctgctgg
    63601 cttgaacaca cttcagccac catcgcgtca tgatacagac gcaccgtcat tgacggaagg
    63661 ctccagtaac tgatcgcggg cgcagtctgt tctattgtca ccagggtagt gtatcgggtc
    63721 gattccacaa tcgtcagccg atattgtgcg tttgccacct gatagcttac agtttcgccg
    63781 ggtgcgtcat tgcgcggtaa caaacggcgc aattgtgaaa agttcatctc gcacaggcgc
    63841 atcatttcag gaaagtcagg tgtgtaacgc ttcatttatg cccactcatt ttttaacgct
    63901 tgatgatgca gctgcagcca ttgcaaagcg atgaccgacg ctgcgttgtc gattttcccc
    63961 tcttctaccc actggtatgc ctgttcccgg cttaccacat gaacgcgaat atcttcgttt
    64021 tcatcagcca gaccatgaat accgcttgcg gtcgtggcgt ccacttcgcc caccataatt
    64081 gacgaacgct cactggtgcc ccccgggctt gccaggaaac ttaacaccgg tttggtccgt
    64141 ttgactatca gtcccgcctc ttcaatcgct tcgcgacggg caacatcttc cacactttca
    64201 ccctcttcaa tcatcccggc aaccatctcc agtagccagg gggtttcgct ggtgtcgtac
    64261 gcggcaatcc gaatctgctc aatcagcaca acttcatcac gcactgggtc aaagggtagc
    64321 aagactgcgg cgtgaccgcg ctcaaaaatt tcccgccgta cctcatgact catttgcccg
    64381 ttgaatagac gatgacgaaa tctataaaga tctaatgaaa aaaagccgcg ataaagtgtt
    64441 tctcgtgcaa taatttctac atcgtttttg ccaaatgtaa cgggcaggtt gtctggctta
    64501 agcattgtta atgtcctagc actaatagtg aattaaatgt gaatttcagc gacgtttgac
    64561 tgccgtttga gcagtcatat gttaaattga ggcacattaa cgccctatgg cacgtaacgc
    64621 caaccttttg cggtagcggc ttctgctaga atccgcaata attttacagt ttgatcgcgc
    64681 taaatactgc ttcaccacaa ggaatgcaaa tgaagaaatt gctccccatt cttatcggcc
    64741 tgagcctttc tgggttcagt tcgttgagcc aggccgagaa cctgatgcaa gtttatcagc
    64801 aagcacgcct tagtaacccg gaattgcgta agtctgccgc cgatcgtgat gctgcctttg
    64861 aaaaaattaa tgaagcgcgc agtccattac tgccacagct aggtttaggt gcagattaca
    64921 cctatagcaa cggctaccgc gacgcgaacg gcatcaactc gaacgcgacc agtgcgtccc
    64981 tgcagttaac tcaatccatt tttgatatgt cgaaatggcg tgcgttaacg ctgcaggaaa
    65041 aagcagcagg gattcaggac gtcacatatc agaccgatca gcaaaccttg atcctcaaca
    65101 ccgcgaccgc ttatttcaac gtgttgaatg ctattgacgt tctttcctat acacaggcac
    65161 aaaaagaagc gatctaccgt caattagatc aaaccaccca acgttttaac gtgggcctgg
    65221 tagcgatcac cgacgtgcag aacgcccgcg cgcagtacga taccgtgctg gcgaacgaag
    65281 tgaccgcacg taataacctt gataacgcgg tagagcagct gcgccagatc accggtaact
    65341 actatccgga actggcggcg ctgaatgtcg aaaactttaa aaccgacaaa ccacagccgg
    65401 ttaacgcgct gctgaaagaa gccgaaaaac gcaacctgtc gctgttacag gcacgcttga
    65461 gccaggacct ggagcgcgag caaattcgcc aggcgcagga tggtcactta ccgactctgg
    65521 atttaacggc ttctaccggg atttctgaca cctcttatag cggttcgaaa acccgtggtg
    65581 ccgctggtac ccagtatgac gatagcaata tgggccagaa caaagttggc ctgagcttct
    65641 cgctgccgat ttatcagggc ggaatggtta actcgcaggt gaaacaggca cagtacaact
    65701 ttgttggtgc cagtgagcaa ctggaaagcg cacatcgtag cgtcgtgcaa accgtacgtt
    65761 cctccttcaa caacattaat gcttctatca gtagtattaa cgcctacaaa caagccgtag
    65821 tttccgcgca aagctcatta gacgcgatgg aagcgggcta ctcggtcggt acgcgtacca
    65881 ttgttgatgt gttggatgcg accaccacgc tgtacaacgc caagcaagag ctggcgaatg
    65941 cgcgttataa ctacctgatt aatcagctga atattaagtc agccctgggt acgttgaacg
    66001 agcaggattt gctggcactg aacaatgcgc tgagcaaacc ggtttccact aatccggaaa
    66061 acgttgcccc gcaaacgccg gaacagaatg ctattgctga tggttatgcg cctgatagcc
    66121 cggcacccgt cgttcagcaa acatccgcac gcactaccac cagtaacggt cataaccctt
    66181 tccgtaactg atgacgacga cggggcttcg gccccgtctg aacgtaaggc aacgtaaaga
    66241 tacgggttat ctgccgcatt cttccccctt ctcgcttcaa tttcgaccag ccatcctcta
    66301 ttctgatggg tatttaccac tggtcccgga agacaaaaat gaaacggaca aaatccatac
    66361 gccacgcatc gttccgcaaa aactggagcg cacgccatct gacaccagtc gctctcgcgg
    66421 ttgccactgt tcttatgctg gctggctgtg aaaagagtga tgaaacagtg tctctctatc
    66481 aaaatgctga cgactgttca gctgcaaacc caggcaaaag cgccgaatgt accaccgcgt
    66541 acaacaatgc gctgaaagaa gccgaacgta ctgcgccgaa atacgccacc cgtgaagact
    66601 gtgttgctga atttggtgaa ggtcagtgtc agcaagcacc agcccaggct ggcatggcac
    66661 cagaaaacca ggcgcaggct cagcaatcca gcgggagttt ctggatgccg ctgatggccg
    66721 gttacatgat ggggcgtctg atgggcggcg gcgcgggatt tgcacagcag ccgctgttct
    66781 cctcgaaaaa cccggccagt ccggcttacg gtaaatatac cgacgcgacg ggtaaaaact
    66841 atggcgcagc ccagccaggc cgcaccatga ccgtaccgaa gacggcaatg gcaccaaaac
    66901 cggcgaccac cactaccgtt acccgtggcg gttttggtga atctgttgcc aaacaaagca
    66961 ctatgcagcg tagcgcaacc ggcacctctt ctcgttcaat gggtggctga taccgatgga
    67021 aagagtcagt attaccgagc gcccggactg gcgtgagaaa gcccacgaat acggtttcaa
    67081 ttttcacacc atgtacggcg agccgtactg gtgtgaagat gcttactaca agttgaccct
    67141 cgcccaggtt gaaaagctgg aagaagtcac cgccgaactg caccagatgt gcctgaaagt
    67201 ggtggaaaaa gtgatcgcca gcgatgagct gatgaccaaa ttccgcatac caaaacacac
    67261 ctggagtttt gtgcgtcaat catggctgac tcaccagcca tcgctttatt cgcgtcttga
    67321 tctggcgtgg gatggcactg gcgaacctaa acttctggaa aataacgccg atacgccaac
    67381 gtcactatac gaggcggcgt tctttcagtg gatctggctg gaagatcagc ttaacgccgg
    67441 taacttgccg gagggcagcg accagtttaa cagtctgcaa gaaaaactga tcgatcgctt
    67501 cgttgagctg cgtgaacagt atggcttcca gttgctgcat ctcacctgct gtcgcgacac
    67561 ggtggaagat cgcggaacca ttcagtattt gcaggactgc gcaaccgaag ctgaaatcgc
    67621 caccgagttc ctctacatcg acgagatagg tttaggcgaa aaaggccagt tcacggattt
    67681 acaggatcag gtgatttcta acctgttcaa gctttatccg tgggaattta tgctgcgcga
    67741 aatgttctcc accaagctgg aagatgctgg cgtacgctgg ctggaaccgg cgtggaagag
    67801 cattatctcc aacaaggcac ttctaccgct actgtgggag atgttcccga atcacccgaa
    67861 cctgctgccc gcttattttg cggaagatga tcatccgcaa atggaaaaat atgtggttaa
    67921 accgatcttc tcccgtgaag gcgcaaacgt gtcgatcatt gagaacggca aaaccattga
    67981 agcagcggaa ggtccgtatg gcgaagaagg gatgattgtt cagcaattcc acccgttacc
    68041 gaaattcggc gacagctata tgctgattgg tagctggctg gtgaacgatc aacctgccgg
    68101 aattggcatt cgtgaagacc gtgcgttgat cacccaggat atgtctcggt tttatccaca
    68161 tatttttgtt gaataagcca cgataccgga tggcactcgc catccggtaa ttgttagcct
    68221 atctgcaccg acagcatact taaactgccc atttctatac catcgaccgg aatggtaatt
    68281 ggctcctgcc catcccacac acctaacaca tacaacaacg gcagatagtg ctctggcgtt
    68341 gggttcgata acgcgccacc ttcatggtcg agataattca ccagaggatg ctgctctacc
    68401 ggcccttgcc acgtcagatt cgctttcaca tactcattaa acgacgtcgc ccacggatac
    68461 ggtgaactat caccgtgcca cttcactgtg cgcaggttat gcaccacgtt accgctggcg
    68521 accaacatta ttccttcatc acgcagcgcc gcgagtttgc gccccatttc gaaatgccag
    68581 gcggcaggtt tgctactgtc gatactcaac tgcaccatag ggatatcggc gtcaggatac
    68641 atcttaatca gcacgcccca cgagccgtgg tcaaagcccc aggcttcttt atccagcgtc
    68701 accgggatcg gcgctaacag ctcaaccaga cgctgtgcca gcgcaggcga acccggagca
    68761 ggataatgcg tatcgtacag cgcctgcggg aagccaccaa agtcatgaat cgtgggcggc
    68821 gtctccatcg cggtcactcc tgttccacgg gtaaaccagt gagccgaaac caccacaatc
    68881 gcttgcgggc gtggcaatgt catccccaac ttctgccagc tgcgggtata caaattatct
    68941 tccagcacgt tcatcggact accgtgacct aaaaacaatg ctggcatacg tgttgaagac
    69001 atgatgatat ccttaactaa aggtgtcatt ttgatatcct cacaatacgc ttgttcggcg
    69061 gagtaagaac ccggataaca atgatgatga tcatcagtta ttttgacgat ctgcctgaag
    69121 gtgaagattt ataaggagtt gtcgatgtca gtacctctca ttctgaccat actggcgggg
    69181 gcagccacgt ttattggcgc gtttctcggc gttctcggac aaaaaccctc gaaccgctta
    69241 ctggcgtttt cgctaggttt tgcggcgggg atcatgttgc tcatctcatt aatggaaatg
    69301 cttcctgccg cactagcagc tgaaggaatg tcgccggtgt tgggttatgg aatgtttatc
    69361 ttcggtctgc ttggctactt tggcctggac cgcatgttgc cgcatgcgca tccgcaggat
    69421 ttaatgcaaa aatcggtgca gctgttgcca aaatcgatca agcgcacagc cattctgctc
    69481 actctcggca tcagtctgca taacttcccg gaagggattg ccacctttgt cacggcgagc
    69541 agcaacctgg agctgggatt tggcatcgca ctggccgtcg cgttgcacaa tatccctgaa
    69601 ggtctggcag tggcaggccc ggtttatgcg gcaacggggt ctaaacgcac cgcgattctg
    69661 tgggcgggga tttctggact agcagaaatt cttggtggtg tgctggcgtg gttaatcctc
    69721 ggtagcatga tttccccggt ggtcatggcg gcaatcatgg cggcggttgc aggaattatg
    69781 gtggcgctct cggttgatga attaatgccg ctcgccaaag agattgaccc taataataac
    69841 cccagctatg gcgtactatg tggaatgtca gtgatgggat tcagtttagt gctgctacaa
    69901 acggcgggaa ttggttaaaa acaacatcag aagttgtcca gcacatggac aacttctgcc
    69961 agcttcacga aagccttatt ctaccttttc ggctgtctct tctctcgtac tgtttaagta
    70021 tttgttccgc gcttcaagca gtgcgtctct gtcctggcga aacgcccgac aataacgttt
    70081 caaatgcagg aaataaccca ccacaaccag agcaatcaat acaatccagt accaggcgat
    70141 aaacatcctt ttacccttaa tatttaacaa tactaaacat ttagcgcata aatttcacat
    70201 atcctttttc ggatatattc atatggtcgc ataataacgg aacaattata tgattttgat
    70261 cataatgaaa aagaatgcct gatattaatg aaattttctc atagaaagag aagcggaatt
    70321 aacatcttgc atagcagggc aaaataaaaa ccgaagccag ctacaacttc ggtttcttga
    70381 ttaaggcagt aattaagcag cggttttcag ctggctttac gctcatgtgc ctgacggtat
    70441 gccaccaggt cttcaatagt cacgagcgcc atattgtgtt tattggcaaa ctcaatgcac
    70501 tctggtgcac gcgccatcgt gccatcgtca ttagtcagct cacacagcac accagccggt
    70561 ttaaagcctg ccagcgtcat caaatcaata gtcgcttcag tatgaccgcc acgcgtcagt
    70621 acaccacctg cctgcgcgcg aagtgggaaa acgtggcccg ggcgattcag atcagaaggt
    70681 tttgcgccat cggcaatcgc tgcgcgaacg gtcgtaatac gatcagcggc agaaacaccg
    70741 gtagtcacac cttcagccgc ttcaatggtc acggtaaaac cggtgccata ggcgctggtg
    70801 ttattttcta ccatcattgg cagatcaagt tgtttacggc gatcttcagt gatgcacagg
    70861 caaacaatac cgctaccgtg gcgaatggtc agcgccatct gctcaacggt catggtttct
    70921 gccgggaaga tcatatcgcc ttcgttttca cggtcttcat catcaagcac cattacaccg
    70981 cgtccttcac gcagcgcagc cagtgcattt tcaacacgtt cgaaaggcgt accaaaagag
    71041 gaaagtagcg tctgattcat ggtaaaaaaa acctcactaa aattatggtt accagaatca
    71101 gggcagtctt aggagtggcg gcatatagcc aaaataacgt gagcgggtcc atgcccgaca
    71161 gaatcgttac tctctcccat ccggactcta accgtcggcc ccggaattac accggatctg
    71221 ctgtcctttg agttcgcacc caaagcgctc gcgggctttc aactgagttg atttaccgcc
    71281 ggtggggaat ttcgccccgc cctgagaata agcggattca ctataacgct aatgattagc
    71341 ggcagcaacg catagcttca cataattctg gtttatgact tacccttatc gcactacaat
    71401 ggtactcaac acctatcagt acagggaaac tacaatgatt gacccgaaaa aaattgagca
    71461 aatcgctcgc caggttcacg aatcaatgcc taaaggaatc agggagttcg gggaagatgt
    71521 ggagaaaaaa atccgccaaa ccctacaagc tcagctgacg cgcctcgacc ttgtaagccg
    71581 tgaagagttc gacgtccaaa cgcaagtcct gttacggact cgtgagaaac tggcgctgct
    71641 tgaacaaaga atcagcgaac tggaaaatcg cagtactgag atcaaaaagc agccagatcc
    71701 agagactctc cccccaacgc tgtaaacatt tgtctttaat gcactgtaaa ttatgaatta
    71761 atcctctaat ttacagtgtt ttctattaca ttatcgctta atcccattca gagtatcatc
    71821 acaaaaatga atgattgcaa ttgaaaatat aattgttatg tattctttaa gttttactta
    71881 atcgata
//
LOCUS       DAJTRC010000006        70710 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.006, whole genome shotgun sequence.
ACCESSION   DAJTRC010000006 DAJTRC010000000
VERSION     DAJTRC010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 70710)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 70710)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..70710
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.006"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..794
                     /locus_tag="OO357_000443"
     CDS             <1..794
                     /locus_tag="OO357_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5885505.1"
                     /translation="LPRSLVSYQMTSQKDRPTQHEMRLDGSLLDDGRLSYSLEQSLDD
                     DNNHNSSLNASYRSPYGTFSAGYSYGNDSSQYNYGVTGGVVIHPHGVTLSQYLGNAFA
                     LIDANGASGVRIQNYPGIATDPFGYAVVPYLTTYQENRLSVDTTQLPDNVDLEQTTQF
                     VVPNRGAMVAARFNANIGYRVLVTVSDRNGKPLPFGALASNDDTGQQSIVDEGGILYL
                     SGISSKSQSWTVRWGNQADQQCQFAFSTPDSEPTTSVLQGTAQCH"
     gene            805..1896
                     /locus_tag="OO357_000444"
     CDS             805..1896
                     /locus_tag="OO357_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5885506.1"
                     /translation="MKRAPLITGLLLISTSCAYASSGGCGADSTSGATNYSSVVDDVT
                     VNQTDNVTGREFTSATLSSTNWQYACSCSAGKAVKLVYMVSPVLTTTGHQTGYYKLND
                     SLDIKTTLQANDIPGLTTDQVVSVNTRFTQIKSSTVYSAATQTGVCQGDTSRYGPVNI
                     GANTTFTLYVTKPFLGSMTIPKTDIAVIKGAWVDGMGSPSTGDFHDLVKLSIQGNLTA
                     PQSCKINQGDVIKVNFGFINGQKFTTRNAMPDGFTPVDFDITYDCGDTSKIKNSLQMR
                     IDGTTGVVDQYNLVARRRSSDNVPDVGIRIENLGGGVANIPFQNGILPVDPSGHGTVN
                     MRAWPVNLVGGELETGKFQGTATITVMVR"
     gene            complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="OO357_000445"
     CDS             complement(1939..2799)
                     /gene="rsmI"
                     /locus_tag="OO357_000445"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HCR5885507.1"
                     /translation="MKQHQSADNSQGQLYIVPTPIGNLADITQRTLEVLQAVDLIAAE
                     DTRHTGLLLQHFGINARLFALHDHNEQQKAETLLAKLQEGQNIALVSDAGTPLINDPG
                     YHLVRTCREAGIRVVPLPGPCAAITALSAAGLPSDRFCYEGFLPAKSKGRRDALKAIE
                     AEPRTLIFYESTHRLLDSLEDIVAVLGESRYVVLARELTKTWETIHGAPVGELLAWVK
                     EDENRRKGEMVLIVEGHKAQEEDLPADALRTLALLQAELPLKKAAALAAEIHGVKKNA
                     LYKYALEQQG"
     gene            2864..4900
                     /gene="lpoA"
                     /locus_tag="OO357_000446"
     CDS             2864..4900
                     /gene="lpoA"
                     /locus_tag="OO357_000446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein activator LpoA"
                     /protein_id="HCR5885508.1"
                     /translation="MVPSTFSRLKAARCLPVVLAALIFAGCGTHTPDQSTAYMQGTAQ
                     ADSAFYLQQMQQSSDDTRINWQLLAIRALVKEGKTGQAVELFNQLPQELNDSQRREKT
                     LLAAEIKLAQKDFAGAQNLLAKITPADLEQNQQARYWQAKIDASQGRPSIDLLRALIA
                     QEPLLGAKEKQQNIDATWQALSSMTQEQANTLVINADENILQGWLDLQRVWFDNRNDP
                     DMMKAGIADWQKRYPNNPGAKMLPTQLVNVKAFKPASTNKIALLLPLNGQAAVFGRTI
                     QQGFEAAKNIGTQPVAAQVAAAPAADVAEQPQPQTADSVASPAQASVSDLTGDQPAAQ
                     PVPVSAPATSTAAVSAPANPSAELKIYDTSSQPLSQILSQVQQDGASIVVGPLLKNNV
                     EELLKSNTPLNVLALNQPENIENRVNICYFALSPEDEARDAARHIRDQGKQAPLVLIP
                     RSALGDRVANAFAQEWQKLGGGTVLQQKFGSTSELRAGVNGGSGIALTGSPITPRATT
                     DSGMTTNNPTLQTTPTDDQFTNNGGRVDAVYIVATPGEIAFIKPMIAMRNGSQSGATL
                     YASSRSAQGTAGPDFRLEMEGLQYSEIPMLAGGNLPLMQQALSAVNNDYSLARMYAMG
                     VDAWSLANHFSQMRQVQGFEINGNTGSLTANPDCVINRKLSWLQYQQGQVVPAS"
     gene            4858..5253
                     /locus_tag="OO357_000447"
     CDS             4858..5253
                     /locus_tag="OO357_000447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HCR5885509.1"
                     /translation="MATVPTRSGSPRQLTTKQTGDAWEAQARRWLEGKGLRFIAANVN
                     ERGGEIDLIMREGLTTVFVEVRYRRSALYGGAAASVTRSKQHKLLQTARLWLARHNGS
                     FDTVDCRFDVVAFTGNEVEWIKDAFNDHS"
     gene            5273..5863
                     /gene="diaA"
                     /locus_tag="OO357_000448"
     CDS             5273..5863
                     /gene="diaA"
                     /locus_tag="OO357_000448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA initiator-associating protein DiaA"
                     /protein_id="HCR5885510.1"
                     /translation="MQERIKACFTESIQTQIAAAEALPDAISRAAMTLVQSLLNGNKI
                     LCCGNGTSAANAQHFAASMINRFETERPSLPAIALNTDNVVLTAIANDRLHDEVYAKQ
                     VRALGHAGDVLLAISTRGNSRDIVKAVEAAVTRDMTIVALTGYDGGELAGLLGPQDVE
                     IRIPSHRSARIQEMHMLTVNCLCDLIDNTLFPHQDD"
     gene            5873..6448
                     /gene="yraP"
                     /locus_tag="OO357_000449"
     CDS             5873..6448
                     /gene="yraP"
                     /locus_tag="OO357_000449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divisome-associated lipoprotein YraP"
                     /protein_id="HCR5885511.1"
                     /translation="MKALSPIAVLISALLLQGCVAAAVVGTAAVGTKAATDPRSVGTQ
                     VDDGTLEVRVNSALSKDEQIKKETRINVTAYQGKVLLVGQSPNAELSARAKQIAMGVD
                     GANEVYNEIRQGQPIGLGEASNDTWITTKVRSQLLTSDLVKSSNVKVTTENGEVFLMG
                     LVTEREAKAAADIASRVSGVKRVTTAFTFIK"
     gene            complement(6562..7602)
                     /locus_tag="OO357_000450"
     CDS             complement(6562..7602)
                     /locus_tag="OO357_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5885512.1"
                     /translation="MTGQSSSQAATPIQWWKPALFFLVVIAGLWYVKWQPYYGKAFTA
                     AETHSIGKSILAQADANPWQAALDYAMIYFLAVWKAAVLGVILGSLIQVLIPRDWLLR
                     TLGQSRFRGTLLGTLFSLPGMMCTCCAAPVAAGMRRQQVSMGGALAFWMGNPVLNPAT
                     LVFMGFVLGWGFAAIRLVAGLVMVLLIATLVQKWVRETPQTQAPVEIDIPEAQSGFFS
                     RWGRALWTLFWSTIPVYILAVLVLGAARVWLFPHADGAVDNSLMWVVAMAVAGCLFVI
                     PTAAEIPIVQTMMLAGMGTAPALALLMTLPAVSLPSLIMLRKAFPAKALWLTGAMVAV
                     SGVIVGGLALLF"
     gene            complement(7675..8310)
                     /locus_tag="OO357_000451"
     CDS             complement(7675..8310)
                     /locus_tag="OO357_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HCR5885513.1"
                     /translation="MSQVLITGATGLVGGHLLRMLINEPKVNAIAAPTRRPLGDMPGV
                     FNPHDPQLSDALAQVTEPIDIVFCCLGTTRREAGSKEAFIHADYTLVVDTALTGRRLG
                     AQHMLVVSAMGANAHSPFFYNRVKGEMEEALIAQNWPKLTIARPSMLLGDRSKQRMNE
                     TLFAPLFRLLPGNWKSIDARDVARVMLAEAMRPEHEGVTILSSSELRKRAE"
     gene            8438..8956
                     /gene="yhbO"
                     /locus_tag="OO357_000452"
     CDS             8438..8956
                     /gene="yhbO"
                     /locus_tag="OO357_000452"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein/nucleic acid deglycase"
                     /protein_id="HCR5885514.1"
                     /translation="MSKKIAVLITDEFEDSEFTSPADEFRKAGHEVITIEKQAGKTVK
                     GKKGEASVTIDKSIDEVTPAEFDALLLPGGHSPDYLRGDNRFVTFTRDFVNSGKPVFA
                     ICHGPQLLISADVIRGRKLTAVKPIIIDVKNAGVEFYDQEVVVDKDQLVTSRTPDDLP
                     AFNREALRLLGA"
     gene            complement(8936..9379)
                     /locus_tag="OO357_000453"
     CDS             complement(8936..9379)
                     /locus_tag="OO357_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhbP family protein"
                     /protein_id="HCR5885515.1"
                     /translation="METLIAISRWLAKQHVVTWCVQQEGELWCANAFYLFDAQKVAFY
                     ILTEEKTRHAQMSGPQAAVAGTVNGQPKTVALIRGVQFKGEIRRLEGEESDLARKAYN
                     RRFPVARMLSAPVWEIRLDEIKFTDNTLGFGKKMIWLRDSGTEQA"
     gene            9430..9732
                     /gene="yhbQ"
                     /locus_tag="OO357_000454"
     CDS             9430..9732
                     /gene="yhbQ"
                     /locus_tag="OO357_000454"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708956.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage response exodeoxyribonuclease YhbQ"
                     /protein_id="HCR5885516.1"
                     /translation="MTPWFLYLIRTADNKLYTGITTDVERRYQQHQSGKGAKALRGKG
                     ELTLAFSAPVGDRSLALRAEYRVKQLTKRQKERLVAEGAGFAELLSSLQTPEIKSD"
     gene            complement(9719..10222)
                     /locus_tag="OO357_000455"
     CDS             complement(9719..10222)
                     /locus_tag="OO357_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5885517.1"
                     /translation="MLIRVEIPIDAPGIDALLRRSFESDAEAKLVHDLREDGFLTLGL
                     VATDDEGQVIGYVAFSPVDVQGEDLQWVGMAPLAVDEKYRGQGLARQLVYEGLDSLNE
                     FGYAAVVTLGDPALYSRFGFELAAHHDLRCRWPGTESAFLVHRLADDALNGVTGLVEY
                     HEHFNRF"
     gene            complement(10216..10740)
                     /locus_tag="OO357_000456"
     CDS             complement(10216..10740)
                     /locus_tag="OO357_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 domain-containing protein"
                     /protein_id="HCR5885518.1"
                     /translation="MLDKLRSRIVHLGPSLLSVPVKLTPFALKRQVLEQVLSWQFRQA
                     LDDGELEFLEGRWLSIHVRDIDLQWFTSVVNGKLVVSQNTQADVSFSADASDLLMIAA
                     RKQDPDTLFFQRRLVIEGDTELGVYVKNLMDAIELEQMPKALRMMLLQLADFVEAGMK
                     TAPETKQTSVGEPC"
     gene            10949..11944
                     /locus_tag="OO357_000457"
     CDS             10949..11944
                     /locus_tag="OO357_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017457364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5885519.1"
                     /translation="MELLCPAGNLPALKAAIENGADAVYIGLKDDTNARHFAGLNFTE
                     KKLQEAVSFVHQHRRKLHIAINTFAHPDGYARWQRAVDMAAQLGADALILADLAMLEY
                     AAERYPHIERHVSVQASATNEEAINFYHRHFDVARVVLPRVLSIHQVKQLARVTPVPL
                     EVFAFGSLCIMSEGRCYLSSYLTGESPNTVGACSPARFVRWQQTPQGLESRLNEVLID
                     RYQDGENAGYPTLCKGRYLVDGERYHALEEPTSLNTLELLPELMAANIASVKIEGRQR
                     SPAYVSQAAKVWRQAIDRCKADPQNFVPQSAWMETLGSMSEGTQTTLGAYHRKWQ"
     gene            11953..12831
                     /locus_tag="OO357_000458"
     CDS             11953..12831
                     /locus_tag="OO357_000458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HCR5885520.1"
                     /translation="MKYSLGPVLWYWPKETLEDFYQQAAASSADVIYLGEAVCSKRRA
                     TKVGDCLEMAKSLAGSGKQIVLSTLALVQASSELGELKRYVENGEFLIEASDLGVVNM
                     CAERKLPFVAGHALNCYNAVTLKILLKQGMMRWCMPVELSRDWLVNLLNQCDELGIRN
                     QFEVEVLSYGHLPLAYSARCFTARSEDRPKDECETCCIKYPNGRNVLSQENQQVFVLN
                     GIQTMSGYVYNLGNELASMQGLVDVVRLSPQGTDTFAMLDAFRANENGAVPLPLTANS
                     DCNGYWRRLAGLELQA"
     gene            12912..13919
                     /locus_tag="OO357_000459"
     CDS             12912..13919
                     /locus_tag="OO357_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5885521.1"
                     /translation="MTDKTIAFSLLDLAPIPEGSSAREAFSHSLDLARLAEKRGYHRY
                     WLAEHHNMTGIASAATSVLIGYLAANTTTLHLGSGGVMLPNHLPLVIAEQFGTLNTLY
                     PGRIDLGLGRAPGSDQRTMMALRRHMSGDIDNFPRDVAELVDWFDARDPNPHVRPVPG
                     YGEKIPVWLLGSSLYSAQLAAQLGLPFAFASHFAPDMLFQALHLYRSNFKPSARLEKP
                     YAMVCINIIAADSNRDAEFLFTSMQQAFVKLRRGETGQLPPPIQNMDQFWSPSEQYDV
                     QQALSMSLVGDKAKVRHGLQSILRETDADEIMVNGQIFDHQARLHSFELAMDVKEELL
                     G"
     gene            complement(14037..15281)
                     /gene="mtr"
                     /locus_tag="OO357_000460"
     CDS             complement(14037..15281)
                     /gene="mtr"
                     /locus_tag="OO357_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan permease"
                     /protein_id="HCR5885522.1"
                     /translation="MATLTTTQTSPSLLGGVVIIGGTIIGAGMFSLPVVMSGAWFFWS
                     MAALIFTWFCMLHSGLMILEANLNYRIGSSFDTITKDLLGKGWNVVNGISIAFVLYIL
                     TYAYISASGSILHHTFAEMSLNVPARAAGFGFALLVAFVVWLSTKAVSRMTAIVLGAK
                     VITFFLTFGSLLGHVQPATLFNVAESNASYAPYLLMTLPFCLASFGYHGNVPSLMKYY
                     GKDPKTIVKCLVYGTLMALALYTIWLLATMGNIPRPEFIGIAEKGGNIDVLVQALSGV
                     LNSRSLDLLLVVFSNFAVASSFLGVTLGLFDYLADLFGFDDSAVGRLKTALLTFAPPV
                     VGGLLFPNGFLYAIGYAGLAATIWAAIVPALLARASRKRFGSPKFRVWGGKPMIALIL
                     VFGVGNALVHILSSFNLLPVYQ"
     gene            complement(15435..17324)
                     /gene="deaD"
                     /locus_tag="OO357_000461"
     CDS             complement(15435..17324)
                     /gene="deaD"
                     /locus_tag="OO357_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417631.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DeaD"
                     /protein_id="HCR5885523.1"
                     /translation="MAEFETTFADLGLKAPILEALNDLGYEKPSPIQAECIPHLLNGR
                     DVLGMAQTGSGKTAAFSLPLLQNLDPELKAPQILVLAPTRELAVQVAEAMTDFSKHMR
                     GVNVVALYGGQRYDVQLRALRQGPQIVVGTPGRLLDHLKRGTLDLSKLSGLVLDEADE
                     MLRMGFIEDVETIMAQIPEGHQTALFSATMPEAIRRITRRFMKEPQEVRIQSSVTTRP
                     DISQSYWTVWGMRKNEALVRFLEAEDFDAAIIFVRTKNATLEVAEALERNGYNSAALN
                     GDMNQALREQTLERLKDGRLDILIATDVAARGLDVERISLVVNYDIPMDSESYVHRIG
                     RTGRAGRAGRALLFVENRERRLLRNIERTMKLTIPEVELPNAELLGKRRLEKFAAKVQ
                     QQLESSDLDQYRALLSKIQPTAEGEELDLETLAAALLKMAQGERTLIVPPDAPMRPKR
                     EFRDRDDRGPRDRNDRGPRGDREDRPRRERRDVGDMQLYRIEVGRDDGVEVRHIVGAI
                     ANEGDISSRYIGNIKLFASHSTIELPKGMPGEVLQHFTRTRILNKPMNMQLLGDAQPH
                     TGGERRGGGRGFGGERREGGRNFSGERREGGRGDGRRFSGERREGRAPRRDDSTGRRR
                     FGGDA"
     gene            complement(17317..17397)
                     /gene="yrbN"
                     /locus_tag="OO357_000462"
     CDS             complement(17317..17397)
                     /gene="yrbN"
                     /locus_tag="OO357_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YrbN"
                     /protein_id="HCR5885524.1"
                     /translation="MKIADQFHDELCRLAAINFEAHVLHG"
     gene            complement(17504..18388)
                     /gene="nlpI"
                     /locus_tag="OO357_000463"
     CDS             complement(17504..18388)
                     /gene="nlpI"
                     /locus_tag="OO357_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020802045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein NlpI"
                     /protein_id="HCR5885525.1"
                     /translation="MKPFLRWCFVATALTLAGCSNTSWRKSEVLAVPLQPTLQQEVIL
                     ARMEQILASRALTDDERAQLLYERGVLYDSLGLRALARNDFSQALAIRPDMPEVFNYL
                     GIYLTQAGNFDAAYEAFDSVLELDPTYNYAHLNRGIALYYGGRDKLAQDDLLAFYQDD
                     PNDPFRSLWLYLAEQKLDEKQAKEVLKQHFEKSDKEQWGWNIVEFYLGNISEQTLMER
                     LKADATDNTSLAEHLSETNFYLGKYYLSLGDLDSATALFKLAVANNVHNFVEHRYALL
                     ELSLLGQDQDDLAESDQQ"
     gene            complement(18497..20632)
                     /gene="pnp"
                     /locus_tag="OO357_000464"
     CDS             complement(18497..20632)
                     /gene="pnp"
                     /locus_tag="OO357_000464"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HCR5885526.1"
                     /translation="MLNPIVRKFQYGQHTVTLETGMMARQATAAVMVSMDDTAVFVTV
                     VGQKKAKPGQDFFPLTVNYQERTYAAGRIPGSFFRREGRPSEGETLIARLIDRPIRPL
                     FPEGFVNEVQVIATVVSVNPQVNPDIVAMIGASAALSLSGIPFNGPIGAARVGYINDQ
                     YVLNPTQDELKESKLDLVVAGTEAAVLMVESEAELLSEDQMLGAVVFGHEQQQVVIQN
                     INELVKEAGKPRWDWQPEPVNEALNARVAALAEARLSDAYRITDKQERYAQVDVIKSE
                     TIATLLAEDETLDENELGEILHAIEKNVVRSRVLAGEPRIDGREKDMIRGLDVRTGVL
                     PRTHGSALFTRGETQALVTATLGTARDAQVLDELMGERTDTFLFHYNFPPYSVGETGM
                     VGSPKRREIGHGRLAKRGVLAVMPDMDKFPYTVRVVSEITESNGSSSMASVCGASLAL
                     MDAGVPIKAAVAGIAMGLVKEGDNYVVLSDILGDEDHLGDMDFKVAGSREGISALQMD
                     IKIEGITKEIMQVALNQAKGARLHILGVMEQAINAPRGDISEFAPRIHTIKINPDKIK
                     DVIGKGGSVIRALTEETGTTIEIEDDGTVKIAATDGEKAKHAIRRIEEITAEIEVGRV
                     YTGKVTRIVDFGAFVAIGGGKEGLVHISQIADKRVEKVTDYLQMGQEVPVKVLEVDRQ
                     GRIRLSIKEATEQSQPAAAPEAPAAEQGE"
     gene            complement(20879..21148)
                     /gene="rpsO"
                     /locus_tag="OO357_000465"
     CDS             complement(20879..21148)
                     /gene="rpsO"
                     /locus_tag="OO357_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HCR5885527.1"
                     /translation="MSLSTEATAKIVSEFGRDANDTGSTEVQVALLTAQINHLQGHFA
                     EHKKDHHSRRGLLRMVSQRRKLLDYLKRKDVARYTQLIERLGLRR"
     gene            complement(21297..22241)
                     /gene="truB"
                     /locus_tag="OO357_000466"
     CDS             complement(21297..22241)
                     /gene="truB"
                     /locus_tag="OO357_000466"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HCR5885528.1"
                     /translation="MSRPRRRGRDINGVLLLDKPQGMSSNDALQKVKRIYNANRAGHT
                     GALDPLATGMLPICLGEATKFSQYLLDSDKRYRVIARLGQRTDTSDADGQIVEERPVT
                     FSAEQLAAALDTFRGDIEQIPSMYSALKYQGKKLYEYARQGIEVPREARPITVYELLF
                     IRHEGNELELEIHCSKGTYIRTIIDDLGEKLGCGAHVIYLRRLAVSKYPVERIVTLEH
                     LRELVEQAEQQDIPAAELLDPLLMPMDSPASDYPVVNLPLTSSVYFKNGNPVRTSGAP
                     LEGLVRVTEGENGKFIGMGEIDDEGRVAPRRLVVEYPA"
     gene            complement(22241..22642)
                     /gene="rbfA"
                     /locus_tag="OO357_000467"
     CDS             complement(22241..22642)
                     /gene="rbfA"
                     /locus_tag="OO357_000467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001712908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HCR5885529.1"
                     /translation="MAKEFGRPQRVAQEMQKEIALILQREIKDPRLGMMTTVSGVEMS
                     RDLAYAKVYVTFLNDKDEDAVKAGIKALQEASGFIRSLLGKAMRLRIVPELTFFYDNS
                     LVEGMRMSNLVTSVVKHDEERRVNPDDSKED"
     gene            complement(22806..25454)
                     /gene="infB"
                     /locus_tag="OO357_000468"
     CDS             complement(22806..25454)
                     /gene="infB"
                     /locus_tag="OO357_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HCR5885530.1"
                     /translation="MTDVTIKTLAAERQTSVERLVQQFADAGIRKSADDSVSAQEKQT
                     LIDHLNQKNSGPDKLTLQRKTRSTLNIPGTGGKSKSVQIEVRKKRTFVKRDPQEAERL
                     AAEEQARREAEESAKREAQQKAEREAAEQAKREAAEQAKREAAEKDKVSNQQDDMTKN
                     AQAEKARREQEAAELKRKAEEEARRKLEEEARRVAEEARRMAEENKWTDNAEPTEDSS
                     DYHVTTSQHARQAEDESDREVEGGRGRGRNAKAARPKKGNKHAESKADREEARAAVRG
                     GKGGKRKGSSLQQGFQKPAQAVNRDVVIGETITVGELANKMAVKGSQVIKAMMKLGAM
                     ATINQVIDQETAQLVAEEMGHKVILRRENELEEAVMSDRDTGAAAEPRAPVVTIMGHV
                     DHGKTSLLDYIRSTKVASGEAGGITQHIGAYHVETENGMITFLDTPGHAAFTSMRARG
                     AQATDIVVLVVAADDGVMPQTIEAIQHAKAAQVPVVVAVNKIDKPEADPDRVKNELSQ
                     YGILPEEWGGESQFVHVSAKAGTGIDELLDAILLQAEVLELKAVRKGMASGAVIESFL
                     DKGRGPVATVLVREGTLHKGDIVLCGFEYGRVRAMRNELGQEVLEAGPSIPVEILGLS
                     GVPAAGDEVTVVRDEKKAREVALYRQGKFREVKLARQQKSKLENMFANMTEGEVHEVN
                     IVLKADVQGSVEAISDSLLKLSTDEVKVKIIGSGVGGITETDATLAAASNAILVGFNV
                     RADASARKVIEAESLDLRYYSVIYNLIDEVKAAMSGMLSPELKQQIIGLAEVRDVFKS
                     PKFGAIAGCMVTEGVVKRHNPIRVLRDNVVIYEGELESLRRFKDDVNEVRNGMECGIG
                     VKNYNDVRTGDVIEVFEIIEIQRTIA"
     gene            complement(25479..26966)
                     /gene="nusA"
                     /locus_tag="OO357_000469"
     CDS             complement(25479..26966)
                     /gene="nusA"
                     /locus_tag="OO357_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005131833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HCR5885531.1"
                     /translation="MNKEILAVVEAVSNEKALPREKIFEALESALATATKKKYEQEID
                     VRVQIDRKSGDFDTFRRWLVVDEVTQPTKEITLEAARYEDESLNLGDYVEDQIESVTF
                     DRITTQTAKQVIVQKVREAERAMVVDQFREHEGEIITGVVKKVNRDNISLDLGNNAEA
                     VILREDMLPRENFRPGDRVRGVLYSVRPEARGAQLFVTRSKPEMLIELFRIEVPEIGE
                     EVIEIKAAARDPGSRAKIAVKTNDKRIDPVGACVGMRGARVQAVSTELGGERIDIVLW
                     DDNPAQFVINAMAPADVASIVVDEDKHTMDIAVEAGNLAQAIGRNGQNVRLASQLSGW
                     ELNVMTVDDLQAKHQAEAHAAIDTFTKYLDIDEDFATVLVEEGFSTLEELAYVPMKEL
                     LEIEGLDEPTVEALRERAKNALATIAQAQEESLGDNKPADDLLNLEGVDRDLAFKLAA
                     RGVCTLEDLAEQGIDDLADIEGLTDEKAGALIMAARNICWFGDEA"
     gene            complement(26994..27446)
                     /gene="rimP"
                     /locus_tag="OO357_000470"
     CDS             complement(26994..27446)
                     /gene="rimP"
                     /locus_tag="OO357_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HCR5885532.1"
                     /translation="MSTLEQKLTEMITAPVEALGFELVGIEFIRGRTSTLRIYIDSED
                     GINVDDCADVSHQVSAVLDVEDPITVAYNLEVSSPGLDRPLFTAEHYARFVGEEVTLV
                     LRMAVQNRRKWQGVIKAVDGEMITVTVEGKDEVFALSNIQKANLVPHF"
     gene            complement(27653..27729)
                     /locus_tag="OO357_000471"
     tRNA            complement(27653..27729)
                     /locus_tag="OO357_000471"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(27693..27695),aa:Met,seq:cat)
     gene            28077..29420
                     /gene="argG"
                     /locus_tag="OO357_000472"
     CDS             28077..29420
                     /gene="argG"
                     /locus_tag="OO357_000472"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HCR5885533.1"
                     /translation="MTTILKHLPVGQRIGIAFSGGLDTSAALLWMRQKGAVPYAYTAN
                     LGQPDEEDYDAIPRRAMEYGAENARLIDCRKQLVAEGIAAIQCGAFHNTTGGLTYFNT
                     TPLGRAVTGTMLVAAMKEDGVNIWGDGSTYKGNDIERFYRYGLLTNAELQIYKPWLDT
                     DFIDELGGRHEMSEFMIACGFDYKMSVEKAYSTDSNMLGATHEAKDLEYLNSSVKIVN
                     PIMGVKFWDESVKIPAEEVTVRFEQGHPVALNGKTFSDDVEMMLEANRIGGRHGLGMS
                     DQIENRIIEAKSRGIYEAPGMALLHIAYERLLTGIHNEDTIEQYHAHGRQLGRLLYQG
                     RWFDSQALMLRDSLQRWVASQITGEVTLELRRGNDYSILNTVSENLTYKPERLTMEKG
                     DSVFSPDDRIGQLTMRNLDITDTREKLFGYAKTGLLSSSATSGLPQVENMENKGQ"
     gene            complement(29428..31035)
                     /locus_tag="OO357_000473"
                     /pseudo
     CDS             complement(29428..31035)
                     /locus_tag="OO357_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011378876.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
     gene            complement(31513..31599)
                     /locus_tag="OO357_000474"
     tRNA            complement(31513..31599)
                     /locus_tag="OO357_000474"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31563..31565),aa:Leu,seq:gag)
     gene            complement(31614..31946)
                     /gene="secG"
                     /locus_tag="OO357_000475"
     CDS             complement(31614..31946)
                     /gene="secG"
                     /locus_tag="OO357_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HCR5885534.1"
                     /translation="MYEALLVVFLIVAIGLVGLIMLQQGKGADMGASFGAGASATLFG
                     SSGSGNFMTRMTALLATLFFIISLVLGNINSNKTNRGSEWENLSAPAKTEQTQPAAPA
                     KPTSDIPN"
     gene            complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="OO357_000476"
     CDS             complement(32174..33511)
                     /gene="glmM"
                     /locus_tag="OO357_000476"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HCR5885535.1"
                     /translation="MSNRKYFGTDGIRGRVGDAPITPDFVLKLGWAAGKVLARHGSRK
                     IIIGKDTRISGYMLESALEAGLAAAGLSALFTGPMPTPAVAYLTRTFRAEAGIVISAS
                     HNPFYDNGIKFFSIDGTKLPDAVEEAIEAEMEKEISCVDSAELGKASRIVDAAGRYIE
                     FCKATFPNELSLSELKIVVDCANGATYHIAPNVLRELGANVIAIGCEPNGVNINAEVG
                     ATDVRALQARVLAEKADLGIAFDGDGDRVIMVDHEGNKVDGDQIMYIIAREGLRQGQL
                     RGGAVGTLMSNMGLELALKQLGIPFARAKVGDRYVLEKMQEKGWRIGAENSGHVILLD
                     KTTTGDGIVAGLQVLAAMARNHMSLHDLCSGMKMFPQILVNVRYTAGSGDPLEHESVK
                     AVTAEVEAALGNRGRVLLRKSGTEPLIRVMVEGEDEAQVTEFAHRIADAVKAV"
     gene            complement(33504..34352)
                     /gene="folP"
                     /locus_tag="OO357_000477"
     CDS             complement(33504..34352)
                     /gene="folP"
                     /locus_tag="OO357_000477"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HCR5885536.1"
                     /translation="MKLFAQGTSLDLSHPHVMGILNVTPDSFSDGGTHNSLIDAVKHA
                     NLMINAGATIIDVGGESTRPGAAEVSVEEELQRVIPVVEAIAQRFEVWISVDTSKPEV
                     IRESAKVGAHIINDIRSLSEPGALEAAAETGLPVCLMHMQGNPKTMQEAPKYDDVFAE
                     VNRYFIEQIARCEQAGIAKEKLLLDPGFGFGKNLSHNYSLLARLAEFHHFNLPLLVGM
                     SRKSMIGQLLNVGPSERLSGSLACAVIAAMQGAHIIRVHDVKETVEAMRVVEATLSAK
                     ENKRYE"
     gene            complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="OO357_000478"
     CDS             complement(34442..36376)
                     /gene="ftsH"
                     /locus_tag="OO357_000478"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HCR5885537.1"
                     /translation="MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNND
                     QVREARINGREINVTKKDSNRYTTYIPVQDPKLLDNLLTKNVKVVGEPPEEPSLLASI
                     FISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEA
                     KEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISG
                     SDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLN
                     QMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVH
                     MRRVPLAPDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKI
                     MMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLP
                     EGDAISASRQKLESQISTLYGGRLAEEIIYGPEHVSTGASNDIKVATNLARNMVTQWG
                     FSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQLLTD
                     NMDILHAMKDALMKYETIDAPQIDDLMARRDVRPPAGWEEPGASNNSGDNGSPKAPRP
                     VDEPRTPNPGNTMSEQLGDK"
     gene            complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="OO357_000479"
     CDS             complement(36476..37105)
                     /gene="rlmE"
                     /locus_tag="OO357_000479"
                     /EC_number="2.1.1.166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uridine(2552)-2'-O)-methyltransferase
                     RlmE"
                     /protein_id="HCR5885538.1"
                     /translation="MTGKKRSASSSRWLQEHFSDKYVQQAQKKGLRSRAWFKLDEIQQ
                     SDKLFKPGMTVVDLGAAPGGWSQYVVTQIGGKGRIIACDLLPMDPIVGVDFLQGDFRD
                     ELVMKALLERVGDSKVQVVMSDMAPNMSGTPAVDIPRAMYLVELALEMCRDVLAPGGS
                     FVVKVFQGEGFDEYLREIRSLFTKVKVRKPDSSRARSREVYIVATGRKP"
     gene            37231..37524
                     /gene="yhbY"
                     /locus_tag="OO357_000480"
     CDS             37231..37524
                     /gene="yhbY"
                     /locus_tag="OO357_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014833438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HCR5885539.1"
                     /translation="MNLSTKQKQHLKGLAHPLKPVVLLGSNGLTEGVLAEIEQALEHH
                     ELIKVKIATEDRETKTLIVEAIVRETGACNVQVIGKTLVLYRPTKERKISLPR"
     gene            complement(37680..38156)
                     /gene="greA"
                     /locus_tag="OO357_000481"
     CDS             complement(37680..38156)
                     /gene="greA"
                     /locus_tag="OO357_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006178768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HCR5885540.1"
                     /translation="MQAIPMTLRGAEKLREELDFLKSVRRPEIIAAIAEAREHGDLKE
                     NAEYHAAREQQGFCEGRIKDIEAKLSNAQVIDVTKMPNNGRVIFGATVTVLNLDSDEE
                     QTYRIVGDDEADFKQNLISVNSPIARGLIGKEEDDVVVIKTPGGEVEFEVIKVEYL"
     gene            38404..39837
                     /gene="dacB"
                     /locus_tag="OO357_000482"
     CDS             38404..39837
                     /gene="dacB"
                     /locus_tag="OO357_000482"
                     /EC_number="3.4.16.4"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001212660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5885541.1"
                     /translation="MRFSRFIIGLTSCIAFSVQAANVDEYVTQLPAGANLALMVQKVG
                     ASAPAIDYHSQQMALPASTQKVITALAALIQLGPDFRFTTTLETKGNVENGVLKGDLV
                     ARFGADPTLKRQDIRNMVATLKKSGVNQIDGNVLIDTSIFASHDKAPGWPWNDMTQCF
                     SAPPAAAIVDRNCFSVSLYSAPKPGDMAFIRVASYYPVTMFSQVRTLPRGSAEAQYCE
                     LDVVPSDLNRFTLTGCLPQRSEPLPLAFAVQDGASYAGAILKDELKQAGITWSGTLLR
                     QTQVNEPGTVVASKQSAPLHDLLKIMLKKSDNMIADTVFRMIGHARFNVPGTWRAGSD
                     AVRQILRQQAGVDIGNTIIADGSGLSRHNLIAPATMMQVLQYIAQHDNELNFISMLPL
                     AGYDGSLQYRAGLHQAGVDGKVSAKTGSLQGVYNLAGFITTASGQRMAFVQYLSGYAV
                     KPADQRNRRIPLVRFESRLYKDIYQNN"
     gene            complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="OO357_000483"
     CDS             complement(40023..41195)
                     /gene="cgtA"
                     /locus_tag="OO357_000483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Obg family GTPase CgtA"
                     /protein_id="HCR5885542.1"
                     /translation="MKFVDEASILVVAGDGGNGCVSFRREKYIPKGGPDGGDGGDGGD
                     VWMEADENLNTLIDYRFEKSFRAERGQNGASRDCTGKRGKDVTIKVPVGTRVIDQGTG
                     ETMGDMTKHGQRLLVAKGGWHGLGNTRFKSSVNRTPRQKTNGTPGDKRELLLELMLLA
                     DVGMLGMPNAGKSTFIRAVSAAKPKVADYPFTTLVPSLGVVRMDNEKSFVVADIPGLI
                     EGAAEGAGLGIRFLKHLERCRVLLHLIDIDPIDGTDPVENARIIISELEKYSQDLAAK
                     PRWLVFNKIDLLDKAEAEEKAKAIAEALGWEDKYYLISAASGLGVKDLCWDVMTFIIE
                     NPVVQAEEAKQPEKVEFMWDDYHRQQLEEIAEEDDEDWDDDWDEDDEEGVEFIYKR"
     gene            complement(41211..42176)
                     /locus_tag="OO357_000484"
     CDS             complement(41211..42176)
                     /locus_tag="OO357_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012135318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5885543.1"
                     /translation="MKQQAGIGILLALTTAICWGALPIAMKQVLEVMEPPTIVFYRFL
                     MASIGLGAILAVKKRLPPLRVFRKPRWLILLAVATAGLFGNFILFSSSLQYLSPTASQ
                     VIGQLSPVGMMVASVFILKEKMRSTQVVGALMLLSGLVMFFNTSLVEIFTKLTDYTWG
                     VIFGVGAATVWVSYGVAQKVLLRRLASPQILFLLYTLCTIALFPLAKPGVIAQLSHWQ
                     LACLIFCGLNTLVGYGALAEAMARWQAAQVSAIITLTPLFTLFFSDLLSLAWPDFFAR
                     PMLNLLGYLGAFVVVAGAMYSAIGHRIWGGLRKHTTVVSQPRAGE"
     gene            complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="OO357_000485"
     CDS             complement(42303..42560)
                     /gene="rpmA"
                     /locus_tag="OO357_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004933559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HCR5885544.1"
                     /translation="MAHKKAGGSTRNGRDSEAKRLGVKRFGGESVLAGSIIVRQRGTK
                     FHAGANVGCGRDHTLFAKADGKVKFEVKGPKNRKFISIEAE"
     gene            complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="OO357_000486"
     CDS             complement(42581..42892)
                     /gene="rplU"
                     /locus_tag="OO357_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015960603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HCR5885545.1"
                     /translation="MYAVFQSGGKQHRVSEGQTVRLEKLDIATGETVEFAEVLMIANG
                     EEVKIGVPFVDGGVIKAEVVAHGRGEKVKIVKFRRRKHYRKQQGHRQWFTDVKITGIS
                     A"
     gene            43150..44121
                     /gene="ispB"
                     /locus_tag="OO357_000487"
     CDS             43150..44121
                     /gene="ispB"
                     /locus_tag="OO357_000487"
                     /EC_number="2.5.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="octaprenyl diphosphate synthase"
                     /protein_id="HCR5885546.1"
                     /translation="MNLEKINELTAQDMAGVNAAILEQLNSDVQLINQLGYYIVSGGG
                     KRIRPMIAVLAARAVGYEGNAHVTIAALIEFIHTATLLHDDVVDESDMRRGKATANAA
                     FGNAASVLVGDFIYTRAFQMMTSLGSLKVLEVMSEAVNVIAEGEVLQLMNVNDPDITE
                     ENYMRVIYSKTARLFEAAAQCSGILAGCTPEEEKGLQDYGRYLGTAFQLIDDLLDYNA
                     DGEQLGKNVGDDLNEGKPTLPLLHAMHHGTPEQAQMIRTAIEQGNGRHLLEPVLEAMN
                     ACGSLEWTRQRAEEEADKAIAALQVLPDTPWREALIGLAHIAVQRDR"
     gene            44348..44626
                     /gene="sfsB"
                     /locus_tag="OO357_000488"
     CDS             44348..44626
                     /gene="sfsB"
                     /locus_tag="OO357_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SfsB"
                     /protein_id="HCR5885547.1"
                     /translation="MESNFIDWHPADIIAGLRKKGTSMAAESRRNGLSSSTLANALSR
                     PWPKGEMIIAKALGTDPWVIWPSRYHDPQTHEFIDRTQLMRSYTKPKK"
     gene            complement(44674..45933)
                     /gene="murA"
                     /locus_tag="OO357_000489"
     CDS             complement(44674..45933)
                     /gene="murA"
                     /locus_tag="OO357_000489"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HCR5885548.1"
                     /translation="MDKFRVQGPTKLQGEVTISGAKNAALPILFAALLAEEPVEIQNV
                     PKLKDVDTSMKLLSQLGAKVERNGSVHIDARDVNVFCAPYDLVKTMRASIWALGPLVA
                     RFGQGQVSLPGGCTIGARPVDLHISGLEQLGATIKLEEGYVKASVDGRLKGAHIVMDK
                     VSVGATVTIMCAATLAEGTTIIENAAREPEIVDTANFLITLGAKISGQGTDRIVIEGV
                     ERLGGGVYRVLPDRIETGTFLVAAAISRGKIICRNAQPDTLDAVLAKLRDAGADIEVG
                     EDWISLDMHGKRPKAVNVRTAPHPAFPTDMQAQFTLLNLVAEGTGFITETVFENRFMH
                     VPELSRMGAHAEIESNTVICHGVEKLSGAQVMATDLRASASLVLAGCIAEGTTVVDRI
                     YHIDRGYERIEDKLRALGANIERVKGE"
     gene            complement(45988..46242)
                     /gene="ibaG"
                     /locus_tag="OO357_000490"
     CDS             complement(45988..46242)
                     /gene="ibaG"
                     /locus_tag="OO357_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BolA family iron metabolism protein IbaG"
                     /protein_id="HCR5885549.1"
                     /translation="MENNEIQSVLMNALSLQEVHVSGDGSHFQVIAVGELFDGMSRVK
                     KQQTVYGPLMEYIADNRIHAVSIKAYTPAEWARDRKLNGF"
     gene            complement(46402..46695)
                     /gene="mlaB"
                     /locus_tag="OO357_000491"
     CDS             complement(46402..46695)
                     /gene="mlaB"
                     /locus_tag="OO357_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance protein MlaB"
                     /protein_id="HCR5885550.1"
                     /translation="MSESLSWMQTGDTLALSGELDQDVLLPLWEMREEAVKGITCIDL
                     SRVSRVDTGGLALLLHLIDLAKKQGNNVTLQGVNDKVYTLAKLYNLPADVLPR"
     gene            complement(46695..47330)
                     /gene="mlaC"
                     /locus_tag="OO357_000492"
     CDS             complement(46695..47330)
                     /gene="mlaC"
                     /locus_tag="OO357_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding protein MlaC"
                     /protein_id="HCR5885551.1"
                     /translation="MFKRLMMVALLVIAPLSAATAADQTNPYKLMDEAAQKTFDRLKN
                     EQPQIRANPDYLRTIVDQELLPYVQVKYAGALVLGQYYKSATPAQREAYFAAFREYLK
                     QAYGQALAMYHGQTYQIAPEQPLGDKTIVPIRVTIIDPNGRPPVRLDFQWRKNSQTGN
                     WQAYDMIAEGVSMITTKQNEWGTLLRTKGIDGLTAQLKSISQQKITLEEKK"
     gene            complement(47349..47900)
                     /gene="mlaD"
                     /locus_tag="OO357_000493"
     CDS             complement(47349..47900)
                     /gene="mlaD"
                     /locus_tag="OO357_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708992.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipid asymmetry maintenance
                     protein MlaD"
                     /protein_id="HCR5885552.1"
                     /translation="MQTKKNEIWVGIFLLAALLAALFVCLKAANVTSIRTESTYTLYA
                     TFDNIGGLKARSPVSIGGVVVGRVADITLDPKTYLPRVTLEIEQRYNHIPDTSSLSIR
                     TSGLLGEQYLALNVGFEDPELGTAILKDGDTIQDTKSAMVLEDLIGQFLYGSKGDDNK
                     NSGDAPAAAPGNNETTEPVGTTK"
     gene            complement(47905..48687)
                     /gene="mlaE"
                     /locus_tag="OO357_000494"
     CDS             complement(47905..48687)
                     /gene="mlaE"
                     /locus_tag="OO357_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014071945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid asymmetry maintenance ABC transporter
                     permease subunit MlaE"
                     /protein_id="HCR5885553.1"
                     /translation="MLLNALASLGHKGIKTLRTFGRAGLMLFNALVGKPEFRKHAPLL
                     VRQLYNVGVLSMLIIVVSGVFIGMVLGLQGYLVLTTYSAETSLGMLVALSLLRELGPV
                     VAALLFAGRAGSALTAEIGLMRATEQLSSMEMMAVDPLRRVISPRFWAGVISLPLLTV
                     IFVAVGIWGGSLVGVSWKGIDSGFFWSAMQNAVDWRMDLVNCLIKSVVFAITVTWISL
                     FNGYDAIPTSAGISRATTRTVVHSSLAVLGLDFVLTALMFGN"
     gene            complement(48695..49504)
                     /gene="mlaF"
                     /locus_tag="OO357_000495"
     CDS             complement(48695..49504)
                     /gene="mlaF"
                     /locus_tag="OO357_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid ABC transporter ATP-binding protein
                     MlaF"
                     /protein_id="HCR5885554.1"
                     /translation="MEQSVANLVDMRDVSFTRGNRCIFDNISLTVPRGKITAIMGPSG
                     IGKTTLLRLIGGQIAPDHGEILFDGENIPAMSRSRLYTVRKRMSMLFQSGALFTDMNV
                     FDNVAYPLREHTQLPAPLLHSTVMMKLEAVGLRGAAKLMPSELSGGMARRAALARAIA
                     LEPDLIMFDEPFVGQDPITMGVLVKLISELNSALGVTCVVVSHDVPEVLSIADHAWIL
                     ADKKIVAHGSAQALQTNPDPRVRQFLDGIADGPVPFRYPAGDYHADLLPGS"
     gene            49714..50679
                     /locus_tag="OO357_000496"
     CDS             49714..50679
                     /locus_tag="OO357_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="calcium/sodium antiporter"
                     /protein_id="HCR5885555.1"
                     /translation="MLLATALLIVGLLLVVYSADRLVFAASILCRTFGIPPLIIGMTV
                     VSIGTSLPEIIVSLAASLHEQRDLAVGTALGSNIINILLILGLAALVRPFTVHSDVLR
                     RELPLMLLVSVVAGSVLYDGQLSRSDGIFLLFLAVLWLLFIVKLARQGTDSLTREQLA
                     ELPRDGGLPVAFLWLGIALIIMPVATRMVVDNATVLANYFAISELTMALTAIAIGTSL
                     PELATAIAGVRKGENDIAVGNIIGANIFNIVIVLGLPALITPGEIDPLAYSRDYSVML
                     LVSIIFALLCWRRSPQPGRGVGVLLTGGFIVWLAMLYWLSPILVE"
     gene            50693..51679
                     /gene="kdsD"
                     /locus_tag="OO357_000497"
     CDS             50693..51679
                     /gene="kdsD"
                     /locus_tag="OO357_000497"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708996.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase KdsD"
                     /protein_id="HCR5885556.1"
                     /translation="MSHVELQPGFDFQQAGKEVLAIERECLAELDQYINQNFTLACEK
                     MFWCKGKVVVMGMGKSGHIGRKMAATFASTGTPSFFVHPSEAAHGDLGMVTPQDVVIA
                     ISNSGESSEITALIPVLKRLHVPLICITGRPESSMARAADVHLCVKVAKEACPLGLAP
                     TSSTTATLVMGDALAVALLKARGFTAEDFALSHPGGALGRKLLLRVNDIMHTGDEIPH
                     VKKTASLRDALLEVTRKNLGMTVICDDNMMIEGIFTDGDLRRVFDMGVDVRQLSIADV
                     MTPGGIRVRPGILAVEALNLMQSRHITSVMVADGDHLLGVLHMHDLLRAGVV"
     gene            51700..52266
                     /gene="kdsC"
                     /locus_tag="OO357_000498"
     CDS             51700..52266
                     /gene="kdsC"
                     /locus_tag="OO357_000498"
                     /EC_number="3.1.3.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate-8-phosphatase KdsC"
                     /protein_id="HCR5885557.1"
                     /translation="MSKAGASLATCYGPVSADVMAKAENIRLLILDVDGVLSDGLIYM
                     GNNGEELKAFNVRDGYGIRCALTSDIEVAIITGRKAKLVEDRCATLGITHLYQGQSNK
                     LIAFSDLLEKLAIAPENVAYVGDDLIDWPVMEKVGLSVAVADAHPLLIPRADYVTRIA
                     GGRGAVREVCDLLLLAQGKLDEAKGQSI"
     gene            52263..52838
                     /gene="lptC"
                     /locus_tag="OO357_000499"
     CDS             52263..52838
                     /gene="lptC"
                     /locus_tag="OO357_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HCR5885558.1"
                     /translation="MSKARRWVIIVLSLAVLVMIGINMAEKDDTAQVVVNNNDPTYKS
                     EHTDTLVYNPEGALSYRLIAQHVEYYSDQAVSWFTQPVLTTFDKDKIPTWSVKADKAK
                     LTNDRMLYLYGHVEVNALVPDSQLRRITTDNAQINLVTQDVTSEDLVTLYGTTFNSSG
                     LKMRGNLRSKNAELIEKVRTSYEIQNKQTQP"
     gene            52807..53364
                     /gene="lptA"
                     /locus_tag="OO357_000500"
     CDS             52807..53364
                     /gene="lptA"
                     /locus_tag="OO357_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide ABC transporter
                     substrate-binding protein LptA"
                     /protein_id="HCR5885559.1"
                     /translation="MKFKTNKLSLNLVLASSLLAASIPAFAVTGDTDQPIHIESDQQS
                     LDMQGNVVTFTGNVIVTQGTIKINADKVVVTRPGGEQGKEVIDGYGKPATFYQMQDNG
                     KPVEGHASQMHYELAKDFVVLTGNAYLQQVDSNIKGDKITYLVKEQKMQAFSDKGKRV
                     TTVLVPSQLQDKNNKGQTPAQKKGN"
     gene            53371..54096
                     /gene="lptB"
                     /locus_tag="OO357_000501"
     CDS             53371..54096
                     /gene="lptB"
                     /locus_tag="OO357_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HCR5885560.1"
                     /translation="MATLTAKNLAKAYKGRRVVEDVSLTVNSGEIVGLLGPNGAGKTT
                     TFYMVVGIVPRDAGNIIIDDDDISLLPLHARARRGIGYLPQEASIFRRLSVYDNLMAV
                     LQIRDDLSAEQREDRANELMEEFHIEHLRDSMGQSLSGGERRRVEIARALAANPKFIL
                     LDEPFAGVDPISVIDIKRIIEHLRDSGLGVLITDHNVRETLAVCERAYIVSQGHLIAH
                     GTPTEILQDEHVKRVYLGEDFRL"
     gene            54144..55577
                     /gene="rpoN"
                     /locus_tag="OO357_000502"
     CDS             54144..55577
                     /gene="rpoN"
                     /locus_tag="OO357_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HCR5885561.1"
                     /translation="MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALESN
                     PLLEQIDTHEEIDTRETQDSETLDTADALEQKEMPEELPLDASWDTIYTAGTPSGTSG
                     DYIDDELPVYQGETTQTLQDYLMWQVELTPFSDTDRAIATSIVDAVDETGYLTVPLED
                     ILESMGDEEIDIDEVEAVLKRIQRFDPVGVAAKDLRDCLLIQLSQFDKTTPWLEEARL
                     IISDHLDLLANHDFRTLMRVTRLKEDVLKEAVNLIQSLDPRPGQSIQTGEPEYVIPDV
                     LVRKHNGHWTVELNSDSIPRLQINQHYASMCNNARNDGDSQFIRSNLQDAKWLIKSLE
                     SRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHS
                     PRGIFELKYFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTSLLSEQG
                     IMVARRTVAKYRESLSIPPSNQRKQLV"
     gene            55600..55887
                     /gene="hpf"
                     /locus_tag="OO357_000503"
     CDS             55600..55887
                     /gene="hpf"
                     /locus_tag="OO357_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome hibernation promoting factor"
                     /protein_id="HCR5885562.1"
                     /translation="MQLNITGNNVEITEALREFVTAKFAKLEQYFDRINQVYVVLKVE
                     KVTHTSDATLHVNGGEIHASAEGQDMYAAIDGLIDKLARQLTKHKDKLKQH"
     gene            56005..56496
                     /gene="ptsN"
                     /locus_tag="OO357_000504"
     CDS             56005..56496
                     /gene="ptsN"
                     /locus_tag="OO357_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HCR5885563.1"
                     /translation="MTNNDTTLQLSSVLNRECTRSRVHCQSKKRALEIISELAAKQLS
                     LPPQVVFEAILTREKMGSTGIGNGIAIPHGKLEEDTLRAVGVFVQLETPIAFDAIDNQ
                     PVDLLFALLVPADQTKTHLHTLSLVAKRLADKTICRRLRAAQSDEELYQIITDTEGTP
                     DEA"
     gene            56542..57396
                     /gene="rapZ"
                     /locus_tag="OO357_000505"
     CDS             56542..57396
                     /gene="rapZ"
                     /locus_tag="OO357_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HCR5885564.1"
                     /translation="MVLMIVSGRSGSGKSVALRALEDMGFYCVDNLPVVLLPDLARTL
                     ADREISAAVSIDVRNMPESPEIFEQAMSNLPDAFSPQLLFLDADRNTLIRRYSDTRRL
                     HPLSSKNLSLESAIDKESDLLEPLRSRADLIVDTSEMSVHELAEMLRTRLLGKREREL
                     TMVFESFGFKHGIPIDADYVFDVRFLPNPHWDPKLRPMTGLDKPVAAFLDRHTEVHNF
                     IYQTRSYLELWLPMLETNNRSYLTVAIGCTGGKHRSVYIAEQLADYFRSRGKNVQSRH
                     RTLEKRKP"
     gene            57393..57665
                     /gene="npr"
                     /locus_tag="OO357_000506"
     CDS             57393..57665
                     /gene="npr"
                     /locus_tag="OO357_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000216792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS phosphocarrier protein NPr"
                     /protein_id="HCR5885565.1"
                     /translation="MTVKQTVEITNKLGMHARPAMKLFELMQGFDAEVLLRNDEGTEA
                     EANSVIALLMLDSAKGRQIEVEATGPQEEEALAAVIALFNSGFDED"
     gene            57878..58510
                     /gene="yrbL"
                     /locus_tag="OO357_000507"
     CDS             57878..58510
                     /gene="yrbL"
                     /locus_tag="OO357_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP regulatory network protein YrbL"
                     /protein_id="HCR5885566.1"
                     /translation="MIRLSEQSPLGTGRHRKCYAHPEDAQRCIKIVYHRGDGGDKEIR
                     RELKYYAHLGRRLKDWSGIPRYHGTVETDCGTGYVYDVIADFDGKPSITLTEFAEQCR
                     YEEDIAQLRQLLKQLKRYLQDNRIVTMSLKPQNILCHRISESEVIPVVCDNIGESTLI
                     PLATWSKWCCLRKQERLWKRFIAQPALAIALQKDLQPRESKTLALTSREA"
     gene            complement(58507..59234)
                     /gene="mtgA"
                     /locus_tag="OO357_000508"
                     /pseudo
     CDS             complement(58507..59234)
                     /gene="mtgA"
                     /locus_tag="OO357_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417675.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monofunctional biosynthetic peptidoglycan
                     transglycosylase"
     gene            complement(59231..59884)
                     /gene="elbB"
                     /locus_tag="OO357_000509"
     CDS             complement(59231..59884)
                     /gene="elbB"
                     /locus_tag="OO357_000509"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenoid biosynthesis glyoxalase ElbB"
                     /protein_id="HCR5885567.1"
                     /translation="MKKIGVILSGCGVYDGSEIHEAVLTLLAISRSGAQAVCFAPDKQ
                     QVDVINHLTGEAMTETRNVLIEAARITRGEIRPLAQADAAELDALIVPGGFGAAKNLS
                     NFASLGSECTVDRELKALAQAMHQAGKPLGFMCIAPAMLPKIFDFPLRLTIGTDIDTA
                     EVLEEMGAEHVPCPVDDIVVDEDNKIVTTPAYMLAQNIAEAASGIDKLVSRVLVLAE"
     gene            complement(60114..62450)
                     /gene="arcB"
                     /locus_tag="OO357_000510"
     CDS             complement(60114..62450)
                     /gene="arcB"
                     /locus_tag="OO357_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic respiration two-component sensor
                     histidine kinase ArcB"
                     /protein_id="HCR5885568.1"
                     /translation="MKQIRLLAQYYVDLMMKLGLVRFSMLLALALVVLAIVVQMAVTM
                     VLHGQVESIDVIRSIFFGLLITPWAVYFLSVVVEQLEESRQRLSRLVQKLEEMRERDL
                     SLNVQLKDNIAQLNQEIAVREKAEAELQETFGQLKIEIKEREETQIQLEQQSSFLRSF
                     LDASPDLVFYRNEDKEFSGCNRAMELLTGKSEKQLVHLKPADVYSPEAAAKVIETDEK
                     VFRHNVSLTYEQWLDYPDGRKACFEIRKVPYYDRVGKRHGLMGFGRDITERKRYQDAL
                     ERASRDKTTFISTISHELRTPLNGIVGLSRILLDTELTAEQEKYLKTIHVSAVTLGNI
                     FNDIIDMDKMERRKVQLDNQPVDFTSFLADLENLSALQAQQKGLRFNLEPTLPLPHQV
                     ITDGTRLRQILWNLISNAVKFTQQGQVTVRVRYDEGDMLHFEVEDSGIGIPQDELDKI
                     FAMYYQVKDSHGGKPATGTGIGLAVSRRLAKNMGGDITVTSEQGKGSTFTLTIHAPSV
                     AEEVDDAFDEDDMPLPALNVLLVEDIELNVIVARSVLEKLGNSVDVAMTGKAALEMFK
                     PGEYDLVLLDIQLPDMTGLDISRELTKRYPREDLPPLVALTANVLKDKQEYLNAGMDD
                     VLSKPLSVPALTAMIKKFWDTQDDEESTVTTEENSKSEALLDIPMLEQYLELVGPKLI
                     TDGLAVFEKMMPGYVSVLESNLTAQDKKGIVEEGHKIKGAAGSVGLRHLQQLGQQIQS
                     PDLPAWEDNVGEWIEEMKEEWRHDVEVLKAWVAKATKK"
     gene            complement(62546..63475)
                     /locus_tag="OO357_000511"
     CDS             complement(62546..63475)
                     /locus_tag="OO357_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709009.2"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HCR5885569.1"
                     /translation="MQLQKLVNMFGGDLTRRYGQKVHKLTLHGGFSCPNRDGTIGRGG
                     CTFCNVASFADEAQQHRSIAEQLAHQANLVNRAKRYLAYFQAYTSTFAEVQVLRSMYQ
                     QAVSQANIVGLCVGTRPDCVPDAVLDLLCEYKDQGYEVWLELGLQTAHDKTLHRINRG
                     HDFACYQRTTQLARQRGLKVCSHLIVGLPGEGQAECLQTLERVVETSVDGIKLHPLHI
                     VKGSIMAKAWEAGRLNGIELEDYTLTAGEMIRHTPPEVIYHRISASARRPTLLAPLWC
                     ENRWTGMVELDRYLNEHGVQGSALGRPWLPPTE"
     gene            64150..68610
                     /gene="gltB"
                     /locus_tag="OO357_000512"
     CDS             64150..68610
                     /gene="gltB"
                     /locus_tag="OO357_000512"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HCR5885570.1"
                     /translation="MLYDKSLERDNCGFGLIAHIEGEPSHKVVRTAIHALARMQHRGA
                     ILADGKTGDGCGLLLQKPDRFFRIVAQERGWRLAKNYAVGMLFLNKDPELAAAARRIV
                     EEELQRETLSIVGWRDVPTNEGVLGEIALSSLPRIEQIFVNAPAGWRPRDMERRLFIA
                     RRRIEKRLEADKDFYVCSLSNLVNIYKGLCMPADLPRFYLDLADLRLESAICLFHQRF
                     STNTVPRWPLAQPFRYLAHNGEINTITGNRQWARARTYKFQTPLIPDLHDAAPFVNET
                     GSDSSSMDNMLELLLAGGMDIIRAMRLLVPPAWQNNPDMDPELRAFFDFNSMHMEPWD
                     GPAGIVMSDGRFAACNLDRNGLRPARYVITKDKLITCASEVGIWDYQPDEVVEKGRVG
                     PGELMVIDTRSGRILHSAETDDDLKSRHPYKEWMEKNVRRLVPFEDLPDEEVGSRELD
                     DDTLASYQKQFNYSAEELDSVIRVLGENGQEAVGSMGDDTPFAVLSSQPRIIYDYFRQ
                     QFAQVTNPPIDPLREAHVMSLATSIGREMNVFCEAEGQAHRLSFKSPILLYSDFKQLT
                     TMKEEHYRADTLDITFDVTKTTLEATVKELCDKAEKMVRSGTVLLVLSDRNIAKDRLP
                     VPAPMAVGAIQTRLVDQSLRCDANIIVETASARDPHHFAVLLGFGATAIYPYLAYETL
                     GRLVDTHAIAKDYRTVMLNYRNGINKGLYKIMSKMGISTIASYRCSKLFEAVGLHDDV
                     VGLCFQGAVSRIGGASFEDFQQDLLNLSKRAWLARKPISQGGLLKYVHGGEYHAYNPD
                     VVRTLQQAVQSGEYSDYQEYAKLVNERPATTLRDLLAITPGENAVNIADVEPASELFK
                     RFDTAAMSIGALSPEAHEALAEAMNSIGGNSNSGEGGEDPARYGTNKVSRIKQVASGR
                     FGVTPAYLVNADVIQIKVAQGAKPGEGGQLPGDKVTPYIAKLRYSVPGVTLISPPPHH
                     DIYSIEDLAQLIFDLKQVNPKAMISVKLVSEPGVGTIATGVAKAYADLITIAGYDGGT
                     GASPLSSVKYAGCPWELGLVETQQALVANGLRHKIRLQVDGGLKTGVDIIKAAILGAE
                     SFGFGTGPMVALGCKYLRICHLNNCATGVATQDDKLRKNHYHGLPFKVTNYFEFIARE
                     TRELMAQLGVTRLVDLIGRTDLLKELDGFTAKQQKLALSKLLETAEPHAGKSLYCTEN
                     NPPFDNGLLNAQLLQQAKPFVDERQSKTFWFDIRNTDRSVGASLSGYIAQTHGDQGLA
                     ADPIKAYFNGTAGQSFGVWNAGGVELYLTGDANDYVGKGMAGGLIAIRPPVGSAFRSH
                     EASIIGNTCLYGATGGRLYAAGRAGERFGVRNSGAITVVEGIGDNGCEYMTGGIVCIL
                     GKTGVNFGAGMTGGFAYVLDESGDFRKRVNPELVEVLSVDDLAIHEEHLRGLITEHVQ
                     HTGSQRGEEILANWSTFATKFALVKPKSSDVKALLGHRSRSAAELRVQAQ"
     gene            68623..70041
                     /gene="gltD"
                     /locus_tag="OO357_000513"
     CDS             68623..70041
                     /gene="gltD"
                     /locus_tag="OO357_000513"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001540975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit GltD"
                     /protein_id="HCR5885571.1"
                     /translation="MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQA
                     DRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDR
                     LCEGSCTLNDEFGAVTIGNIERYINDKAFEMGWRPDMSGVKQTGKKVAIIGAGPAGLA
                     CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTE
                     VGRDVQLDDLLSDYDAVFLGVGTYQSMRGGLENEDADGVYAALPFLIANTKQLMGFGE
                     TRDEPFVSMEGKRVVVLGGGDTAMDCVRTSVRQGAKHVTCAYRRDEENMPGSRREVKN
                     AREEGVEFKFNVQPLGIEVNGNGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHIVPA
                     DAVIMAFGFRPHNMEWLAKHSVELDSQGRIIAPEGNDNAFQTSNPKIFAGGDIVRGSD
                     LVVTAIAEGRKAADGIMNWLEV"
     gene            70225..70704
                     /locus_tag="OO357_000514"
     CDS             70225..70704
                     /locus_tag="OO357_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005085993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1016 family protein"
                     /protein_id="HCR5885572.1"
                     /translation="MESLSERTSTGYQQIHNGIIHLVDSARTETVRSVNALMTATYWE
                     IGRRIVEFEQGGEARAAYGAQLIDRLSKDLSRRYKRGVSAKNLRQMRLFYLFFQHVEI
                     RQTVSGELTPLGIPQTPSAEFPSAKIWQTLSAKLRGCLIFPQLLYCISYFRFVYSCK"
BASE COUNT        17314 a        19176 c        17559 g        16661 t
ORIGIN      
        1 ggttaccgcg tagcctggtt tcctatcaga tgaccagcca gaaagatcgc ccaacccaac
       61 atgaaatgcg tcttgatggc tcactgctgg atgatggtcg cctgagctat agcctggaac
      121 aaagtctgga tgacgataac aaccataaca gtagcctgaa cgccagttac cgttcacctt
      181 atggaacctt cagtgccgga tacagttacg gtaatgacag cagccaatac aattacggcg
      241 ttaccggcgg cgtggttatc catcctcatg gcgtgacgct ctcgcaatat ctgggcaacg
      301 cttttgcgct tatcgatgct aacggggctt ctggcgtgag gatacaaaac tatccgggga
      361 ttgctactga tccctttggc tatgcagtgg ttccttatct cacgacttat caggaaaacc
      421 gtctctcggt agatactacg cagctgcccg ataacgtcga tcttgaacaa acaacacagt
      481 ttgtggtgcc caacagaggt gcaatggtag cggcgcgttt caacgccaat atcggttatc
      541 gcgtacttgt tacagtcagc gatcgcaacg gtaaaccgtt gccctttggc gctcttgcca
      601 gcaacgatga tacggggcaa caaagtatcg tcgatgaggg cggcatacta tatctctctg
      661 ggatatcgag taaatcacaa agctggactg tacgctgggg aaatcaggca gatcaacaat
      721 gtcagtttgc ttttagtaca ccggattcag aaccaacaac ctctgtatta caaggcacag
      781 cgcagtgcca ttaaggataa aaaaatgaaa agagcgcctc ttataacagg acttttgttg
      841 atatccacat cctgcgctta tgcctcctca ggagggtgtg gagccgacag cactagcggt
      901 gcgacaaatt acagcagtgt ggttgatgat gttacggtga accagacaga taacgtgaca
      961 ggacgggagt ttacctctgc aacgctaagt agcactaact ggcaatacgc ctgttcctgc
     1021 tctgcgggta aggcagttaa acttgtctat atggtcagcc ccgtacttac caccactgga
     1081 catcagacag gatattacaa actcaatgac agcctggata ttaaaaccac attacaggca
     1141 aacgacattc caggactcac aaccgaccag gttgtctctg ttaacacccg attcacacag
     1201 ataaaaagca gcaccgtata ttctgctgca acccaaacgg gtgtttgcca gggtgacacg
     1261 tctcgttatg gacccgttaa tattggtgca aataccacct ttaccctgta tgtcaccaag
     1321 ccatttctcg gctcgatgac cattccgaaa acggatattg ccgtcattaa aggcgcgtgg
     1381 gtcgatggaa tgggaagccc gtctacaggt gacttccatg atttagtcaa gttatcgatt
     1441 cagggaaatc tcaccgcccc acagtcgtgc aaaattaatc agggcgatgt tattaaagtt
     1501 aattttggat tcatcaatgg tcagaagttt accacccgca atgccatgcc agacggtttt
     1561 actccagtag actttgatat cacttatgac tgtggtgata cttcaaagat taaaaactcg
     1621 ttgcaaatgc gcatcgacgg tacaactggg gtagtagacc agtacaacct ggtcgccagg
     1681 cgaagaagtt cagacaatgt gcccgatgtc ggtattcgta ttgaaaatct cggcggcgga
     1741 gttgcaaata ttccttttca gaacggtatc cttcccgttg atccttccgg gcatggcacc
     1801 gtcaatatgc gcgcctggcc agttaatctg gtcggtggtg agctggaaac aggaaaattt
     1861 cagggcacag ccaccattac cgtcatggtg cggtaaaaga agacgttgcg ttttgattaa
     1921 cgcaacgctt cggcactgtt acccctgctg ctccagcgca tacttataca gcgcattttt
     1981 cttcacgcca tgaatttctg cggccagcgc cgccgctttt ttcagcggca gttctgcctg
     2041 tagcagcgcc agcgtgcgca gggcatcggc gggtaagtct tcttcctgtg ctttatgacc
     2101 ttcgacaatc agcaccattt cgcctttgcg acggttttca tcttccttta cccacgccag
     2161 cagctcgcca acgggcgcgc cgtgaatggt ttcccaggtt ttggtcagct cacgcgccag
     2221 aaccacgtag cgggattcgc ctaataccgc aacgatatct tccaggctat ctaacagacg
     2281 gtgggtagat tcataaaaaa tcagcgtgcg cggctccgct tcaatggctt ttagcgcatc
     2341 acggcggcct tttgatttgg caggtaaaaa gccttcgtaa cagaaacggt cagatggtaa
     2401 acccgcggcg cttaacgcag tgatagcagc acacggtccg ggtagcggca ccacacgaat
     2461 ccccgcttca cggcaggtac gcaccagatg gtagccagga tcgttaatta gcggcgttcc
     2521 ggcatcggaa accagcgcaa tgttttgccc ctcttgcagc tttgccagca gcgtttcggc
     2581 tttttgttgt tcgttatggt cgtgcagcgc aaacaaccgg gcattaatcc caaaatgttg
     2641 cagcaataaa ccggtgtgac gagtatcctc ggcggcaatc agatcaacgg cctgtaatac
     2701 ctctaacgta cgctgggtga tatccgccag attgccgatt ggcgtcggta caatgtaaag
     2761 ctggccctga gaattatccg ccgattggtg ttgtttcatt gtgtcgtccg tattgccgat
     2821 ttaatattga gcattgcgta aaaaaaatat cactggatac attatggtac cctcaacatt
     2881 ttctcgtttg aaagccgcgc gttgtctgcc tgttgttctg gcagccctga ttttcgccgg
     2941 ttgtggcacc catactcccg atcagtccac tgcttatatg cagggcacgg cgcaggctga
     3001 ttctgccttt tatcttcagc agatgcagca aagctctgat gataccagga tcaactggca
     3061 attactcgcc attcgtgcac tggtgaaaga aggtaaaacc gggcaggcgg ttgagttgtt
     3121 taaccaacta ccgcaagaac tgaacgattc tcagcgtcgc gagaaaacac tgctggcggc
     3181 agagattaaa ctggcgcaga aagattttgc tggcgcgcaa aacttgctgg cgaaaatcac
     3241 acctgccgat ttagaacaaa accagcaagc gcgttactgg caggcaaaaa tcgatgccag
     3301 ccaggggcgt ccttccattg atttactgcg cgcgttaatt gctcaggaac cgctgcttgg
     3361 cgcgaaagaa aaacagcaga atattgatgc cacctggcag gcgctctcct ccatgactca
     3421 ggaacaggcg aatacgctgg tgatcaacgc cgacgaaaat attctgcaag gctggctgga
     3481 tctgcagcgc gtctggtttg ataaccgtaa cgatcccgac atgatgaaag ccgggatcgc
     3541 cgactggcag aaacgttatc cgaacaatcc gggcgcgaaa atgctgccaa cgcagttggt
     3601 taacgtaaaa gcgtttaaac cagcctcgac caacaaaatc gccctgctgt tgccgctgaa
     3661 tggccaggca gcggtatttg gtcgcactat tcagcaaggc tttgaagccg cgaaaaatat
     3721 cggcactcag ccagtggcgg ctcaggtagc tgccgcacct gccgcagacg tagcagaaca
     3781 acctcagccg caaactgcgg atagcgttgc cagcccggca caagcctcgg ttagcgatct
     3841 gaccggtgat cagcctgcgg cccagccggt gcctgtaagc gccccggcga caagcaccgc
     3901 agcggtaagc gcacccgcaa atccatccgc agagctgaaa atctacgata cctcatcaca
     3961 accacttagc cagatcttaa gccaggttca gcaggatggc gcgagtattg tggttggtcc
     4021 gttgctgaaa aataacgttg aagagttgct gaagagcaac accccgctga acgtactggc
     4081 actgaaccag ccggagaata tcgaaaaccg cgtcaatatt tgttacttcg cgctttcacc
     4141 ggaagacgaa gcgcgcgatg cagcgcgtca tattcgtgac cagggtaaac aagcgccgct
     4201 ggtgctgatc ccacgcagtg cattgggcga tcgcgtagcc aatgcgtttg cgcaagagtg
     4261 gcagaaactg ggcggcggca ccgttctgca acaaaaattt ggttccacca gcgaattacg
     4321 cgcgggtgtt aacggcggtt ctggtattgc tttaacgggt agcccgatta ctcccagagc
     4381 gacaaccgac tccggcatga cgaccaacaa tccaacgctg caaaccacgc caaccgatga
     4441 ccagttcacc aataatggcg gtcgtgtcga tgcggtctac attgtggcaa cgccgggtga
     4501 aatcgctttt atcaaaccga tgatcgccat gcgtaacggt agccagagcg gtgcaacgct
     4561 gtacgccagt tcccgcagcg cacaaggcac cgcaggcccg gatttccgac tggagatgga
     4621 aggcttgcag tacagcgaaa tcccgatgct ggcaggcggt aatctgccgt taatgcagca
     4681 ggcgctcagc gcggtgaaca acgattattc gctggctcgc atgtatgcga tgggcgttga
     4741 tgcctggtcg ttggcaaatc atttctcaca aatgcgccag gttcagggtt ttgaaatcaa
     4801 cggtaatacc ggaagtctga cggctaaccc ggattgcgtg attaacagga agttatcatg
     4861 gctacagtac caacaaggtc aggtagtccc cgccagttaa ccaccaaaca gaccggcgat
     4921 gcgtgggaag cacaagcgcg tcgctggctg gaaggcaaag gactgcggtt tatcgccgct
     4981 aacgtgaacg agcgtggcgg cgagatcgat ctgataatgc gtgaaggcct gaccaccgtt
     5041 tttgtcgagg tacgctatcg ccgctctgcg ctttatggcg gcgcggcagc cagtgtgacc
     5101 cgcagcaaac aacacaaatt attacagact gcccgcttgt ggctcgcgcg tcataatggg
     5161 agttttgata ctgtggattg ccggttcgat gtggtagcct tcaccgggaa tgaggttgag
     5221 tggattaagg atgcctttaa tgaccactca taattaaggt ttaaggatta gcgtgcaaga
     5281 aagaattaaa gcttgcttca ctgaaagcat tcaaactcaa attgcggcgg cagaggcgct
     5341 tccggatgcc atctcccgtg cagccatgac gctggttcag tctctgctca atggcaacaa
     5401 aatcctctgt tgtggtaatg gaacttccgc tgccaatgca cagcattttg ctgccagcat
     5461 gatcaaccgt ttcgaaacgg agcggcccag cttacctgcc attgcactaa atactgataa
     5521 tgttgtctta acggcgattg ccaacgatcg cttacatgat gaagtgtatg caaaacaggt
     5581 gcgggcgctg ggtcatgcgg gagatgtatt gttagccatt tccacccgtg gcaacagccg
     5641 cgatattgtt aaagcagttg aagccgccgt tacgcgtgat atgaccattg tggcattgac
     5701 cggctatgac ggcggcgaac ttgcaggttt gttagggcca caggatgtgg agatccgcat
     5761 tccttcgcat cgtagtgctc gcattcagga aatgcatatg ctgacggtaa attgcctgtg
     5821 cgatctgatc gataacacgc ttttccctca ccaggatgat taaggagaat acatgaaggc
     5881 attatcgcca atcgcagtcc ttatttccgc gctgctgttg caaggttgtg ttgccgctgc
     5941 cgtagtgggt accgctgctg tgggtaccaa agccgcaact gacccacgca gtgtcggcac
     6001 ccaggtggac gatggtaccc tggaagtgcg cgtgaacagc gcattgtcga aagacgaaca
     6061 gattaagaaa gaaacccgta ttaatgtcac ggcctatcag ggcaaagtgc tgctggttgg
     6121 gcagtcacca aatgctgaac tttcggctcg cgccaaacag attgctatgg gcgtagacgg
     6181 tgccaacgaa gtgtataacg agattcgtca gggccagccg attggtctgg gcgaagcatc
     6241 taacgatacg tggatcacca ccaaagtgcg ttcgcagctc ttaaccagcg acctggtgaa
     6301 atcgtccaac gtgaaagtga ccaccgaaaa cggtgaagtg ttcctgatgg ggctggtgac
     6361 tgaacgtgaa gcgaaagcgg cggcagatat tgccagccgg gtgagcggcg taaagcgggt
     6421 taccacagcg tttacgttta ttaaatagca gccccttgta atgcctgatg cgacgcttgc
     6481 cgcgtcttat caggcctaca acgatgcaaa ccgtaggtcg gataaggcgt ttacgccgca
     6541 tccgacatgt tttccctcaa atcagaacaa cagcgccagc ccgccgacaa tcacaccaga
     6601 cactgccacc atcgcccctg tcagccataa ggctttcgcc gggaacgctt tgcgcagcat
     6661 aatcagtgac ggcaaactca ctgccgggag cgtcatcaac aatgccagcg ccggagcggt
     6721 tcccatacct gccagcatca tcgtttgtac aatcggaatt tctgctgccg tgggaatgac
     6781 aaacaagcat cctgctaccg ccatcgccac cacccacatc aggctgttat cgacagcacc
     6841 atcggcatgg gggaataacc agacgcgtgc ggcacccaac accagtactg caaggatgta
     6901 aaccgggatc gtactccaga aaagtgtcca tagcgccctg ccccagcggc taaaaaaacc
     6961 gctctgtgct tccggtatgt caatttcgac cggtgcctgc gtttgcggtg tttcacgcac
     7021 ccatttttgc accagcgtcg caatcagcaa caccatcacc agcccggcca ccagacgaat
     7081 cgccgcaaaa ccccagccga ggacaaaccc cataaacacc agcgtcgccg ggtttaacac
     7141 cggattgccc atccagaatg ccagcgcacc gcccatcgat acctgttggc gacgcattcc
     7201 cgccgcgacc ggagccgcac agcaggtaca catcatgccc ggcagcgaaa acagcgttcc
     7261 cagcagcgtg ccgcgaaagc gcgattgccc aagcgtacgc aacaaccagt cacgcgggat
     7321 cagcacctga atcaacgaac cgaggatcac ccccagcacc gccgctttcc atactgcgag
     7381 gaaatagatc atcgcgtaat ccaacgccgc ttgccacgga ttagcatccg cctgcgcaag
     7441 gatagattta ccgatactgt gggtttcggc tgcagtaaac gctttgccgt agtaaggttg
     7501 ccatttcaca taccagagac cggcaatgac aacgagaaag aaaagcgcgg gtttccacca
     7561 ctgaatgggc gttgccgcct gagatgaaga ctgaccagtc atagcattcc ccaggaataa
     7621 gttatgataa ttgagcgcgt gaatattacg ctcactatca attcttggga ataattattc
     7681 agctcttttg cgtaattctg aagagcttaa aatcgtcacg ccttcatgct caggccgcat
     7741 cgcctctgcc agcatgaccc gcgccacgtc tctcgcatca atggatttcc agttacctgg
     7801 taacaaacgg aacagcggcg caaaaagcgt ttcgttcatc cgctgtttgc tacgatcgcc
     7861 cagtaacatc gacgggcgag caatggtcaa tttcggccag ttctgggcaa ttaatgcttc
     7921 ttccatctcc cctttgacgc ggttatagaa aaacggcgag tgggcattgg cacccatagc
     7981 actgaccacc agcatatgct gcgcgcccag tcgccgcccg gttaatgcgg tatccactac
     8041 cagcgtgtaa tcggcatgaa taaacgcctc tttgctcccc gcttctcgcc gcgtggtgcc
     8101 gagacaacaa aacacaatgt cgatgggttc ggtgacctgc gccaacgcgt cactcagttg
     8161 cggatcatgg ggattaaaca ccccgggcat atcgcccaac ggacgtcgcg tcggcgcagc
     8221 aatggcgtta actttcggtt cattaatcaa catccgcagc aggtgaccgc ccaccagccc
     8281 cgttgcgcct gtaatcagta cctgactcat cttcgttcct ttacagaatt gtctgccttg
     8341 cgctccccgg ctcatgcacc agtcttaata gaccatgagg taattatccc cgattgtgga
     8401 aaattcgcct catccaatgc aacaacgcgg aggaagcatg agtaagaaaa ttgccgtttt
     8461 aatcactgat gaatttgaag attcagaatt tacttcaccc gcagacgagt tccgtaaagc
     8521 cggacacgaa gtgattacta ttgaaaaaca agcgggtaaa acggtgaaag gcaaaaaagg
     8581 agaagccagc gtgaccatcg ataaatccat cgatgaagtg acgccagcgg agtttgatgc
     8641 cctgttgcta ccgggtggtc attcaccgga ttatctgcgt ggcgacaacc gttttgtcac
     8701 ctttacccgt gattttgtga atagcggcaa acctgttttc gccatctgtc acggtccgca
     8761 gctgctgatc agcgccgatg tgattcgggg gcgcaaactg accgcagtta aaccgatcat
     8821 tattgatgtt aaaaatgcgg gcgtggaatt ttacgatcag gaagtcgtgg ttgataaaga
     8881 tcagctggtt accagccgga caccggacga tctgccagcg tttaaccgcg aggcgttacg
     8941 cctgctcggt gcctgagtcg cgtagccaaa tcattttttt accaaagccc agcgtgttgt
     9001 cggtgaattt gatttcatcg agccggattt cccacaccgg ggccgacagc attctggcaa
     9061 ccggaaagcg acgattgtac gccttgcgcg cgaggtcgct ttcctcacct tccagcctgc
     9121 ggatctcacc tttaaactgc acaccgcgaa ttaacgctac cgttttcggc tgaccgttta
     9181 ccgttccggc aaccgcagcc tgcggcccac tcatctgcgc gtggcgcgtt ttttcttccg
     9241 tcaaaatgta gaaggcgact ttctgcgcat caaaaagata aaaggcatta gcgcaccaga
     9301 gttccccttc ctgctgcaca caccaggtga caacatgttg ttttgccagc caacggctga
     9361 tggcgatgag tgtttccatt gctgttctct tttatactgt gggcctgacc ttaacatgcg
     9421 atgcagacga tgacaccctg gtttctttac ttgatccgta ccgccgacaa taagctttat
     9481 accgggatca ccacggatgt cgaacgccgc tatcagcagc accaaagcgg caaaggggcg
     9541 aaagcactgc gcgggaaagg agaactgacg ctggcgtttt ctgcgccagt gggcgaccgt
     9601 tcgctggcac tacgggcgga atatcgcgtt aagcaactga ccaaacggca aaaagagcgt
     9661 ctggtagcgg aaggcgcagg gtttgcagaa ctgttgagca gcctgcaaac cccggagatt
     9721 aaaagcgatt gaaatgctcg tgatactcaa ccagaccggt tacgccattc agcgcgtcat
     9781 ccgctaaacg atgtaccagg aaggcgcttt cagtgcccgg ccagcggcaa cgcagatcgt
     9841 gatgcgccgc cagttcaaag ccgaaacgac tgtacagcgc cggatcgccc agcgtcacca
     9901 ctgcggcata gccgaactca ttaagcgaat cgagtccttc atagaccagt tggcgtgcca
     9961 gcccctgtcc acggtatttt tcatcaaccg ccaacggtgc catgccgacc cattgcaggt
    10021 cttcgccctg cacatcaacc ggactaaatg ccacatagcc aatgacctga ccttcgtcat
    10081 ctgtcgccac cagccccagc gtcagaaagc catcttcacg cagatcgtga accagcttcg
    10141 cttccgcatc actttcgaat gagcgacgca gcagggcatc aatacccggc gcatcaatgg
    10201 gaatttctac tcgaattagc atggttcacc taccgatgtc tgtttggttt caggcgcggt
    10261 tttcattccc gcctcaacaa aatccgccag ttgcagcagc atcatgcgta aggctttcgg
    10321 catttgctcc agttcaatgg cgtccatcag gtttttcaca tacaccccca gctccgtatc
    10381 gccttcaatc accagccgac gctggaagaa gagcgtatcc ggatcttgtt tacgcgccgc
    10441 aatcatcagc agatcgctgg cgtcggcact aaaactcaca tcagcttgcg tgttctggct
    10501 gaccaccagt ttgccattca ccaccgaggt aaaccattgc aggtcaatat cacgcacatg
    10561 aatacttaac cagcggcctt caagaaactc cagctcgcca tcatccagcg cctgacggaa
    10621 ttgccagctt aagacctgct caagaacctg gcgttttagc gcaaatggcg tcagttttac
    10681 cggtacactc aacagagatg gccccaaatg cacaatacgg gaacgcagtt tatccaacac
    10741 gagttttact ccctgtttca acaatcatcc tattttgcca tatcagaaaa ataacatagc
    10801 ggtataaatc aacaattcca tatgaaattg ctgctaccac caatacaact ttaactgcct
    10861 taaatcaaaa attgtcgcag caaggttaac taaaatccca gttcgttaac atttttgcgt
    10921 tttgatagcg caaccttcag gaaaaattat ggagctgctc tgccctgccg gaaatctccc
    10981 ggcgcttaag gcggccatcg aaaacggcgc agatgctgtt tatatcgggc taaaagatga
    11041 taccaatgcc cgtcacttcg ccggccttaa ctttaccgag aaaaaattgc aggaagcggt
    11101 gagttttgtc catcaacatc gccgcaaact tcacatcgcg attaacactt ttgcgcatcc
    11161 ggacggttac gcccgttggc agcgtgccgt ggatatggcg gcgcagctgg gtgccgacgc
    11221 gctgatcctc gccgacctcg ccatgctgga gtacgccgcc gagcgttacc cgcatattga
    11281 acgtcatgtg tcagtgcagg cttcggcgac caatgaagag gcgattaact tttatcatcg
    11341 ccattttgac gttgcccgcg tggtgctgcc gcgcgtgttg tcgattcatc aggtgaaaca
    11401 gctggcacgg gttactcctg tcccgctgga agtgtttgct ttcggcagcc tgtgtattat
    11461 gtcggaaggc cgttgctatc tgtcgtcgta tctgacgggt gagtcgccca acactgtggg
    11521 tgcgtgttct ccggcccgtt tcgtgcgctg gcaacaaacg ccgcaggggc tggaatcccg
    11581 cctgaacgaa gtgctgatcg accgttatca ggacggcgaa aacgcaggtt atccgacgct
    11641 gtgtaaaggg cgttatctgg tggacggcga gcgctatcac gcgctggaag aaccaaccag
    11701 tctcaatacc ctggaactgc tgccggagtt aatggctgcg aatattgctt cggtgaaaat
    11761 tgaaggccgc cagcgtagcc cggcgtatgt cagccaggcg gcgaaagtct ggcgtcaggc
    11821 tatcgaccgt tgtaaggccg atccgcaaaa cttcgtaccg caaagcgcgt ggatggagac
    11881 gctcgggtcg atgtccgaag gcacgcaaac cacccttggc gcgtatcacc gtaaatggca
    11941 gtgagaagag caatgaaata ttccttaggg ccagtgctgt ggtactggcc aaaagagacg
    12001 ctggaagatt tttatcagca agccgccgcc agcagcgccg acgtgattta tcttggtgaa
    12061 gcggtatgca gcaagcgccg ggcaaccaaa gttggcgact gtctggagat ggcaaaatcg
    12121 ctcgccggaa gtggcaagca gattgtgctc tccacgctgg cgctggtgca ggcatcctct
    12181 gaactgggcg aactgaaacg ctatgttgag aacggtgaat ttctgattga agccagcgat
    12241 ctcggcgtgg tgaatatgtg cgctgaacgc aaactgccgt tcgtcgccgg gcacgcgctg
    12301 aactgctaca acgcggtgac gctgaaaata ttgctcaaac agggcatgat gcgctggtgt
    12361 atgccggtgg agctttcccg cgactggctg gtgaatctgc ttaatcagtg cgatgagctg
    12421 ggcattcgca accagtttga agtggaagtc ctgagctacg gtcatctacc gctggcctac
    12481 tccgcccgct gctttaccgc gcgttcggaa gaccgcccga aagatgagtg tgaaacctgc
    12541 tgcattaagt atccgaacgg gcgcaacgtg ctgtcgcagg aaaaccaaca agtgtttgta
    12601 ctcaatggca ttcagaccat gagcggctac gtttacaacc tcggtaacga gctggcatcc
    12661 atgcagggat tggttgatgt agtccgcctg tcaccgcagg gcactgacac tttcgcgatg
    12721 ctcgacgcct tccgcgctaa tgaaaatggc gctgtgccac tgccgttgac ggcaaacagc
    12781 gactgtaacg gctactggcg gcggctggca ggactggagc tgcaagccta agtaaatagc
    12841 tcactttgtt aacaacttta actattcttt aatgcagtat taaagattaa tcggtaacaa
    12901 agtgagctgt tatgactgat aaaaccattg cgttttcgct actcgatctg gcccccatac
    12961 ccgaaggctc ttcagcgcgg gaagcgttct cccactctct cgatctcgcc cgtctggctg
    13021 aaaagcgcgg ctatcatcgc tactggctgg cagaacacca caatatgacc ggcattgcca
    13081 gtgctgcaac atcggtattg attggctatc tggcggcgaa taccaccacg ctgcatctgg
    13141 ggtctggcgg cgtgatgttg cctaaccact taccgttggt cattgccgaa cagttcggca
    13201 cactcaatac gctctatccg gggcgaatcg atttggggct gggtcgtgct ccgggtagtg
    13261 accaacggac aatgatggcg ctacgtcgtc atatgagcgg cgatattgat aatttccccc
    13321 gcgatgtcgc ggagctggtg gactggtttg acgcccgcga tcccaatccg catgtgcgcc
    13381 cggtaccagg ctatggcgag aaaatccccg tgtggttgtt aggctccagc ctttacagtg
    13441 cacaactggc ggcgcagctt ggtctgccat ttgcgtttgc ctcacacttc gcgccggata
    13501 tgttgttcca ggcgctgcat ctttatcgca gtaacttcaa accgtcggct cggctggaaa
    13561 aaccatacgc gatggtgtgc atcaatatta tcgccgccga cagcaaccgc gacgctgaat
    13621 ttctgtttac ctcaatgcag caagcctttg tgaagctgcg ccgtggcgaa accgggcaac
    13681 tgccgccgcc gattcaaaat atggatcagt tctggtcacc gtctgagcag tatgacgtgc
    13741 agcaggcgct gagtatgtcg ttggtaggcg ataaagcgaa agtgcgtcat ggtttgcagt
    13801 cgatcctgcg cgaaaccgac gctgatgaga ttatggttaa cgggcagatt ttcgaccacc
    13861 aggcgcgcct gcattcgttt gagctggcga tggatgttaa ggaagagttg ttgggatagt
    13921 gtgtcttaac gcgggaagcc ttatccgagc tggcaacgct gtcctacgta ggcctgataa
    13981 gcgaagcgca tcaggcattg tgtaggcagc agaaatgtcg gataaggcac cgctgattac
    14041 tgatacaccg gcagtaaatt aaagctcgat aaaatatgca ccagtgcgtt gccgacgcca
    14101 aacaccagaa tcagcgcaat catcggcttg ccaccccaga cgcggaattt cgggctgcca
    14161 aagcgtttac gcgatgcgcg ggccagcagt gccggaacaa ttgccgccca gatggtagcc
    14221 gctaaaccag cataaccaat ggcatacagg aatccgttcg ggaacagcag accacccaca
    14281 acaggcgggg caaaggtcag caatgccgtt ttcaagcggc ccacagccga gtcgtcgaaa
    14341 ccaaacagat ctgccagata gtcaaacaaa cccagcgtta cgccaaggaa cgaactcgct
    14401 accgcaaagt ttgagaacac gaccagcaga aggtccaggc tacggctgtt cagcacgccg
    14461 cttaatgctt gtaccaacac atcaatatta ccgcccttct cggcgatgcc gataaactcc
    14521 ggacgaggga tgttgcccat tgtcgccagc aaccagatgg tatacagcgc cagtgccatc
    14581 agcgtaccgt acaccaggca tttcacgatg gttttcggat ctttgccgta atacttcatc
    14641 aggcttggca cgttaccgtg ataaccaaac gacgccaggc agaacggcag ggtcatcagc
    14701 aaatacggcg cataagatgc attgctttcg gcaacgttga acaatgtcgc aggctgcaca
    14761 tgccccagca ggctaccgaa ggtgaggaag aaggtaatga ctttcgcccc cagcacaatc
    14821 gccgtcatgc ggctgacggc tttagtactc aaccacacca caaacgctac cagcagtgca
    14881 aaaccaaaac ccgccgcccg tgccgggacg tttagcgaca tctctgcgaa ggtgtgatgc
    14941 agaatcgaac cactggcaga aatataggca taggtcagga tatagagcac aaaggcaatg
    15001 gaaatgccgt tgaccacgtt ccagcctttg cccagcaaat ctttggtgat ggtgtcaaaa
    15061 ctcgaaccga ttcggtaatt caggttagct tccagaatca tcaagccgga atgcagcata
    15121 cagaaccagg taaagatcag cgctgccatt gaccagaaaa accacgcccc ggacatgacc
    15181 actggcagag aaaacatccc tgcgccaata atggtgccgc cgataatcac cacgccgcca
    15241 agcagcgacg gtgacgtttg ggtggtggtt agtgttgcca tgagggcttc tctccagtga
    15301 aaaatagtgc gactgcgttg ttatgcattg cactgtacca gtacatgagt acaaaagaca
    15361 gaaaaaaagc cccgatggtt aaaatcgggg ctgtatatat tattttacag attgtgttcg
    15421 ctgttcagcg atgattacgc atcaccaccg aaacgacgac gaccggtaga gtcatcacga
    15481 cgcggtgcgc ggccttcacg acgttcgccg ctaaaacgac gaccatcacc acgaccacct
    15541 tcacggcgtt caccgctgaa gttacgaccg ccttcacgac gctcgccacc gaaaccacga
    15601 ccaccgccac gacgctcacc gccagtatgc ggctgtgcat cgcccagtaa ctgcatgttc
    15661 atcggcttgt tgagaatgcg agtgcgcgta aagtgttgca gcacttcacc cggcatacct
    15721 ttcggcagtt cgatggtgga gtgagaagca aacagcttga tgttaccgat gtaacggctg
    15781 ctgatgtcgc cttcgttagc aatcgcgcca acgatatgac gaacttcaac accatcatcg
    15841 cggcccactt caatgcggta cagctgcata tcgccaacat cacgacgttc acgacgcgga
    15901 cgatcttcac ggtcgccacg cgggccacgg tcgttacgat cgcgcggacc acggtcatca
    15961 cggtcacgga attcacgttt cggacgcatc ggcgcatctg gcggaacaat cagagtacgt
    16021 tcaccctgtg ccattttcag cagtgccgca gccagagttt cgagatccag ctcttcacct
    16081 tctgcagtcg gctgaatttt gctcagcagt gcgcggtatt gatccagatc gctgctttcc
    16141 agctgctgct gtactttagc ggcgaatttt tccagacggc gtttgcccag cagttctgcg
    16201 ttcggcagtt ctacttccgg aatagtcagc ttcatagtac gttcaatgtt gcgcagcaga
    16261 cgacgctcgc ggttctcaac gaacagcagc gcgcggccag cacgacccgc acgaccggta
    16321 cgaccgatac ggtgaacgta agactcagaa tccatcggga tatcgtagtt aaccaccagg
    16381 ctgatacgct caacgtccag gccacgggcc gcaacgtcgg tcgcaatcag gatgtccaga
    16441 cgaccgtctt taaggcgttc cagtgtctgc tcacgcagcg cctggttcat gtcaccgttc
    16501 agcgcggcgc tattgtagcc gttacgctca agagcttcag ccacttccag agtcgcgttt
    16561 ttggtacgaa cgaagataat cgccgcatca aaatcttccg cttccaggaa acgtaccagt
    16621 gcttcgtttt tacgcatacc ccacacagtc cagtagctct ggctgatgtc aggacgggta
    16681 gtcacgctgg actgaatacg cacttcctgc ggctctttca taaagcggcg ggtaatgcga
    16741 cgaatcgctt ccggcatggt tgcagagaac agagcggtct gatgaccttc cgggatctgc
    16801 gccataatgg tttcaacgtc ttcgatgaag cccatgcgca gcatttcgtc agcttcatcc
    16861 agaaccagac cgctcagttt agagaggtcc agagtgccac gtttcaggtg gtccagcaga
    16921 cggcccggag taccgacaac gatctgcggc ccctgacgca gggcgcgtaa ttgcacgtca
    16981 taacgctggc cgccgtacag ggcaaccaca tttacgccgc gcatgtgttt agagaaatcc
    17041 gtcattgctt cagcaacctg taccgccagt tcgcgggtcg gtgccagcac cagaatctgt
    17101 ggtgctttca gctcaggatc aagattctgc agcagaggta aagagaatgc tgcagttttt
    17161 ccgctccccg tctgggccat acccagaacg tcgcggccat tcagcagatg tggaatacac
    17221 tctgcctgaa ttggagatgg tttttcgtaa cccagatcgt taagggcttc aaggatagga
    17281 gccttcaggc ccagatctgc aaaagtggtt tcgaattcag ccatgtagta cgtgtgcctc
    17341 aaaattaatg gcggccagtc tacataactc atcatgaaat tgatcagcaa ttttcattga
    17401 aaagtgtgaa ccggctcaaa gtaggtgtat taacgaacaa caacgccctc acccgttaag
    17461 gtgatggcaa tcaaaaaaga ttacgggctg atgtgtacgt cagctattgc tggtccgatt
    17521 ctgccaggtc atcttggtcc tggcccagga gcgataattc caacaatgcg tatcggtgct
    17581 caacaaagtt atgaacgttg ttggcaaccg ccagtttgaa cagtgccgtg gcgctgtcca
    17641 aatcccccag acttaggtag tacttaccta aatagaagtt ggtttcactg agatgctcag
    17701 cgagcgaggt gttatccgtt gcgtccgcct tgagcctttc cattaacgtt tgttcgctaa
    17761 tgttgcccag gtagaactcg acaatgttcc atccccactg ttccttatcc gatttttcga
    17821 agtgctgttt caatacttct ttagcctgct tctcatcgag cttctgctcg gcgagataaa
    17881 gccacagact acggaaagga tcattgggat cgtcttgata aaacgccagc agatcatctt
    17941 gcgctaactt gtcacgaccg ccgtaatata atgcaatccc gcgattcaag tgcgcgtagt
    18001 tgtaagttgg atcaagctca agtacagaat caaacgcttc ataggcagca tcaaaattgc
    18061 ctgcctgcgt taaatatatg cctaagtaat tgaatacttc aggcatatcc ggtcggattg
    18121 ccagcgcttg cgaaaaatcg ttacgcgcta atgccctcag accgagacta tcatacaaca
    18181 ctccgcgctc atataaaagc tgtgcgcgtt cgtcatcggt taaagcccga ctggcaagga
    18241 tttgttccat acgtgccaga atcacttcct gctgtaaagt cggttgcaat ggtaccgcga
    18301 ggacttcact tttacgccag gaagtattac tgcatcctgc aagcgtaagt gctgtcgcaa
    18361 cgaaacacca gcgcaaaaaa ggcttcattt cccactcccg aagaccacgg ttgaatgaac
    18421 gtcctgttcc cggttgctaa caaggcgtcc tgcccggtta aaagcccccc gccgcagcgg
    18481 agggcaaatg gcaaccttac tcgccctgtt cagcagccgg agcttccggt gctgcagcag
    18541 gttgagactg ctcagtcgct tctttaatgc tcagacggat acggccctgg cgatcaactt
    18601 ccagaacttt caccggtact tcctgaccca tctgcaggta atcggtcact ttctcaacgc
    18661 gtttgtcagc gatttgagag atgtgtacca gaccttcttt accgccgccg atggcaacaa
    18721 atgcgccaaa gtcaacgata cgggtcactt taccagtgta gacgcggccc acttcgattt
    18781 ctgcagtgat ctcttcgata cgacgaatag catgtttcgc tttctcgccg tcggtcgctg
    18841 caatcttcac agtaccgtca tcttcgattt cgatggtggt gccagtttct tcggtcaggg
    18901 cacggataac agagccgcct ttaccgataa cgtctttgat cttgtccggg ttgatcttga
    18961 tggtatggat acgtggtgcg aactcagaga tatcgccacg cggcgcgttg atcgcctgtt
    19021 ccattacgcc caggatatgc agacgcgcac ctttagcctg gttcagcgca acctgcatga
    19081 tctctttggt gataccttca attttgatat ccatctgcaa tgcagagata ccctcgcggg
    19141 aacccgcaac tttgaagtcc atatcgccca ggtgatcttc gtcgcccaaa atgtcagaca
    19201 gtacaacgta gttgtcgcct tctttcacca gacccattgc gatacccgca acggcagctt
    19261 tgatcggcac acctgcgtcc atcagcgcca gagacgcgcc gcacacggaa gccatagaag
    19321 aggaaccgtt ggattcagtg atttcagaca caacacgtac ggtgtacggg aatttgtcca
    19381 tatccggcat gactgccagc acgccgcgct tcgccagacg accgtgacca atttcacgac
    19441 gcttcggaga accgaccatg ccggtttcgc ctacggagta cggagggaag ttgtagtgga
    19501 acaggaaggt atcggtacgt tcgcccatca gttcatcaag aacctgcgcg tcacgagcag
    19561 tacccagcgt tgcggtaacc agcgcctgcg tttcaccacg ggtgaacagc gcagaaccgt
    19621 gagtacgcgg cagcacgcca gtacgcacat ccagaccacg gatcatatct ttttcacgac
    19681 cgtcgatacg cggttcgcct gccagtacgc ggctacgaac aacgtttttc tcgatcgcgt
    19741 gcagaatttc acccagttcg ttttcgtcca gggtttcgtc ttcagcaagc agcgtcgcga
    19801 tggtttcaga tttgatgaca tcaacctgcg cataacgctc ttgtttgtcg gtgatgcggt
    19861 aagcatcgct caggcgagct tcagccagtg cagcaacgcg cgcgttcagc gcttcgttta
    19921 ccggctccgg ctgccagtcc caacgtggtt taccggcttc tttcaccagt tcattgatgt
    19981 tctgaataac aacctgctgt tgttcatgac cgaacactac tgcgcccagc atctggtctt
    20041 cgctcagcag ttcagcttca gattcaacca tcagtacagc ggcttcagta ccggcaacaa
    20101 ccagatccag tttgctctct ttcaactcgt cctgagtcgg gttcagtacg tactggtcat
    20161 tgatgtaacc tacgcgagca gcaccaatcg gaccattgaa cggaatacca gacagagaca
    20221 gtgctgcgga agcaccaatc atcgcgacga tatccgggtt aacttgcggg ttaacagaaa
    20281 ccacggtggc gataacctga acttcgttga cgaagccttc cgggaacagc ggccgaatcg
    20341 ggcggtcaat cagacgcgcg atcagggttt cgccttcgct tgggcggcct tcacgacgga
    20401 agaagctacc cgggatacga ccagcagcgt aggtacgctc ctgatagtta acggtcagtg
    20461 ggaagaagtc ctgacctggt ttggcttttt tctggccaac aacggtaaca aataccgcgg
    20521 tgtcatccat gctaaccata acagcggcag tagcctgacg agccatcatg ccggtttcca
    20581 gagtcacggt gtgttggccg tactggaatt tacgaacgat cggattaagc aatgtaatat
    20641 cctttctctt tcttagacag taccttacgg cactggtgtt aatacccgat cttctgcgca
    20701 tcctcgcgac taatgacaac cctaacccag ctctatgtgg gtaaagcctc tcattagccg
    20761 cgcgaacctc tgcaacggaa gatcattcat agcaacaata cattagtttc cagtgaattg
    20821 ctgccgtcag cttgaaaaaa ggggccactc aggccccctt ttctgaaact cgcaagaatt
    20881 agcgacgcag acccaggcgc tcgatgagct gggtgtaacg tgctacgtct ttacgtttca
    20941 ggtagtcgag cagtttacga cgctgagaaa ccatgcgcag cagaccacga cggctgtggt
    21001 gatctttttt gtgctctgca aagtggccct gcaggtggtt gatctgtgca gtcagcagtg
    21061 ctacctgaac ttcagtagaa ccggtgtcgt ttgcgtcacg accaaactca gaaacgattt
    21121 tagctgttgc ttcagtactt agagacattt taaaactcca aagtatatag aatgaaagga
    21181 cgccgatctc taattcagcg atcccagtgt acgttacgca aagtgttaaa caatttacgc
    21241 gacgttaagc ggcagtattc tactcgtagc gacctgttat cgcaagacgg ttaacattac
    21301 gccgggtatt caaccaccag gcgacgaggc gcaacgcggc cttcatcgtc aatttcgccc
    21361 ataccgataa atttgccgtt ctcaccttct gtaacgcgaa ccagtccttc cagcggcgca
    21421 ccggatgtac gaactgggtt accatttttg aagtaaacag aagacgttaa cggaagattc
    21481 accaccgggt agtccgaagc tggactgtcc attggcatca gtaatggatc aagtaactcc
    21541 gcagccggaa tatcctgctg ttcggcttgt tcgaccagtt cacgcagatg ttccagggtc
    21601 acaatccgtt caaccggata tttacttacc gccagacgac gcaggtaaat aacatgcgcg
    21661 ccacaaccga gtttttcacc caggtcatca atgatggtgc ggatataagt gccttttgag
    21721 cagtgaattt ccagctccag ctcattgcct tcgtggcgaa taaacagcaa ttcataaacg
    21781 gtaatcgggc gcgcttcacg cggtacttca atgccctgac gcgcatattc gtacagtttt
    21841 ttaccctgat atttcagtgc tgaatacatc gaagggatct gttcgatatc gccacggaaa
    21901 gtatccagcg ccgccgccag ttgctctgcg ctaaaggtta ccggacgttc ttcaacgatt
    21961 tgtccgtcgg catcagaagt atcggtacgc tgtccaagac gcgcaatgac ccgatagcgt
    22021 ttgtcggagt ccagcagata ctgggaaaac ttcgtcgctt ccccgaggca aattggcaac
    22081 atgccagttg ccagcgggtc cagcgcaccg gtatgcccgg cacggttggc gttataaatc
    22141 cgtttcactt tttgcagcgc atcattgctg gacataccct gcggtttatc cagcaacaaa
    22201 acgccgttaa tgtcgcgacc gcgacgacga ggacgactca ttagtcctcc ttgctgtcgt
    22261 ccgggttaac acgacgttct tcgtcatgtt tgaccacgct ggtcaccagg tttgacatgc
    22321 gcatcccttc aaccagagag ttgtcgtaga agaaggtcag ttccggcacg atacgcaggc
    22381 gcatcgcttt ccccagcagg ctgcggatga aaccagaagc ttcttgcaac gctttgatgc
    22441 ccgctttaac cgcgtcttca tctttgtcgt tgaggaacgt cacatatact ttggcatacg
    22501 ccaggtcgcg agacatttcg acaccggaaa cggtggtcat catgcccagg cgaggatctt
    22561 taatttcacg ctgcaggatg agagcgatct ctttttgcat ttcctgcgct acgcgctgcg
    22621 ggcgaccaaa ttctttcgcc ataataaatt ctcctgacaa aaaaggggct gttagcccct
    22681 ttttaaaatt aatttcaggt ggaagggctg ttcacgttgg cctgataaga cgcgccagcg
    22741 tcacatcagg caatccatgc cggatgcggc gtaaacgcct tatcccgcat ggaaccctaa
    22801 aaaccttaag cgatggtacg ttggatctcg atgatttcga atacttcgat cacatcgcca
    22861 gtgcggacgt cgttgtagtt cttaacgccg ataccacatt ccataccgtt acggacttcg
    22921 ttaacgtcat ctttgaagcg gcgcagggac tccagctcgc cttcgtagat aaccacgttg
    22981 tcacgcagaa cgcggatcgg gttgtgacgt ttaaccacac cttcggtaac catacagcct
    23041 gcgatggcac caaatttcgg cgatttgaac acgtcacgaa cttccgccag accgataatc
    23101 tgctgtttca gttccggaga cagcataccg ctcatcgccg ctttcacttc gtcaatcagg
    23161 ttatagatga cggagtagta acgcagatcc aggctttccg cttcaatcac tttacgtgca
    23221 gaggcatcag cacgtacgtt gaagccaacc aggatggcgt tggacgccgc agccagggtg
    23281 gcgtcggttt cggtgatacc acctacgcca gaaccgatga tcttcacttt aacttcgtca
    23341 gtagacagtt tcagcaagga gtcggagatc gcttccacag aaccctgtac gtctgccttc
    23401 aggacgatgt tcacttcgtg aacttcgcct tcggtcatgt tggcgaacat gttctcgagt
    23461 ttagatttct gctgacgcgc cagtttaact tcgcggaatt taccctgacg atacagtgca
    23521 acttcacgcg ctttcttctc gtcacgtaca acggtaactt catcacccgc agccggtacg
    23581 ccggacaggc cgaggatttc caccggaatg gacggacccg cttccagcac ttcctgaccc
    23641 agttcgttac gcatcgcacg aacacgaccg tattcgaagc cacacagaac gatatcgccc
    23701 ttgtgcagag taccttcacg taccagaacg gtagcaaccg gaccacgacc tttatcgagg
    23761 aaggattcga taaccgcacc gctcgccata cctttacgta ccgctttcag ctccagaact
    23821 tccgcctgca gcaggatagc gtccagcagt tcatcgatac cagtacccgc tttcgcagac
    23881 acatgtacga actggctttc accgccccac tcttccggca ggatgccgta ctgggagagt
    23941 tcgttcttaa cgcgatccgg atcggcttct ggtttatcga tcttgttcac tgcaacgacc
    24001 accggaacct gcgccgcttt cgcgtgctgg attgcttcga tcgtctgcgg cattacgccg
    24061 tcgtcggcag caacaaccag gactacgatg tccgttgcct gcgcaccacg agcacgcatt
    24121 gaagtaaacg cggcgtgccc cggggtgtcc aggaaggtga tcatgccgtt ttcagtttca
    24181 acgtggtatg caccaatgtg ctgggtaatg ccgcccgctt cgccagaggc cactttcgtt
    24241 gaacgaatgt agtccagcag agaggtttta ccgtggtcaa cgtgacccat gatggtcaca
    24301 accggcgcgc gcggttcagc cgcagcaccc gtgtcacggt cgctcattac cgcctcttcc
    24361 agctcgtttt cacgacgcag gataacttta tggcccatct cttcagcaac cagctgtgcg
    24421 gtttcctgat cgataacctg gttgatggtt gccattgcgc ccagtttcat catcgctttg
    24481 atgacctgag agcctttaac cgccatcttg ttcgccagtt cgccaacggt gatagtttcg
    24541 ccgatcacaa cgtcacggtt aacggcctga gcaggcttct ggaagccttg ctgcagcgaa
    24601 gaacctttac gttttccgcc tttaccgcca cgtactgctg cgcgtgcttc ttcacgatca
    24661 gcttttgatt cagcgtgttt gttgcctttc ttcggacgcg ctgctttcgc gttacgacca
    24721 cggccacggc cgccttcgac ttcacgatcg ctttcgtctt ctgcctggcg agcatgttga
    24781 gaagtagtga cgtgataatc gctggaatct tcagtcggtt ccgcgttatc agtccatttg
    24841 ttttcttccg ccatacgacg tgcttcttca gcaacgcgac gtgcttcttc ttcgagttta
    24901 cgacgcgctt cttcttcagc tttacgcttg agctctgcag cttcctgctc acggcgggct
    24961 ttttcagcct gggcgttttt agtcatatcg tcttgttgat tgctcacttt gtctttttcc
    25021 gcagcttcac gtttcgcttg ttcagcagct tcacgcttag cttgttctgc ggcctcacgt
    25081 tcagcttttt gttgcgcctc gcgtttagcc gattcttctg cctcacgacg ggcttgctct
    25141 tccgctgcaa ggcgttcagc ctcttgcgga tcgcgtttca caaaggtgcg tttcttgcgg
    25201 acttcgattt gtaccgattt gctttttcca ccggtaccag gaatgttaag ggtgctgcgt
    25261 gttttacgtt gcagcgtcaa tttgtccggg cctgaatttt tctgattcag gtggtcaatc
    25321 aaagtctgtt tctcttgtgc agacacagag tcgtcagcag acttccggat acctgcatca
    25381 gcaaattgct gtaccaggcg ttccacggag gtctgtcgct ctgcggccag cgttttaatc
    25441 gttacatctg tcatgctgtt ccttcctgct acagtttatt acgcttcgtc accgaaccag
    25501 caaatattac gggcagccat aatcagtgct ccggcttttt cgtcggtcaa cccttcgata
    25561 tcagccagat catcaatgcc ctgttcggcg agatcttcca gcgtacaaac gccacgggcg
    25621 gccagtttga atgccaaatc acgatctacc ccttcaaggt tcagcagatc gtcagccggt
    25681 ttgttatcac cgaggctttc ttcctgggcc tgtgcaatgg tggccagtgc atttttagca
    25741 cgctcgcgca gtgcttcaac ggtcggctca tcaaggcctt cgatttccaa cagctctttc
    25801 atcggcacat aggccagttc ttccagcgtc gagaaacctt cttctaccag aacagtcgcg
    25861 aagtcttcgt cgatgtcgag atatttggtg aaggtgtcga tcgctgcgtg cgcttccgcc
    25921 tgatgcttag cctgcaggtc gtcaacggtc attacgttga gttcccaacc gctcagctgc
    25981 gaagccagac gcacgttctg accgttacgg ccaatcgcct gcgccaggtt accagcttca
    26041 acggcgatat ccatggtgtg tttatcttca tccaccacga tagaagcaac gtctgccggt
    26101 gccattgcgt taatcacaaa ctgcgccggg ttatcatccc acaggacgat atcgatacgc
    26161 tcgccgccca gttcagtaga aaccgcctga acacgcgcgc cacgcatacc tacgcaagca
    26221 cctaccggat cgatacgttt atcgttggtt ttcaccgcga ttttcgcacg agaacccggg
    26281 tcgcgagccg ctgctttaat ttcaatcact tcttcgccga tttccggcac ttcaatacgg
    26341 aacagttcga tcagcatttc cggcttggaa cgagtgacga acagttgcgc gccacgcgct
    26401 tccgggcgaa cggaataaag cacgccacga acgcggtcgc cagggcggaa gttttcacgc
    26461 ggcagcatat cttcgcgcag gatcacggct tcagcgttgt tgcccagatc cagagagatg
    26521 ttgtcgcggt ttactttttt caccacgccg gtgatgattt caccttcgtg ttcacggaac
    26581 tgatcaacca ccatcgcacg ttcggcttca cgcactttct gcacgataac ctgttttgcc
    26641 gtctgggtgg tgatacggtc aaaggtaaca gactcaatct gatcttcaac gtaatcgccc
    26701 aagttcaggc tttcatcttc ataacgtgcg gcttcaaggg tgatttcctt ggtcggctgg
    26761 gtgacttcat caacaactaa ccagcgacgg aaggtgtcaa aatcaccgct tttgcgatcg
    26821 atctgtacac ggacgtcgat ctcttgctca tattttttct ttgttgctgt cgccagcgcg
    26881 ctttccaatg cttcgaaaat cttctcgcga ggtagcgcct tttcattgga tacggcttca
    26941 actacagcca aaatttcttt gttcatcgcg ggcttttcac ctcatccaga ctattaaaag
    27001 tggggaacca ggttcgcctt ctggatatta ctcagcgcga acacttcatc tttaccttcg
    27061 acggtaactg tgatcatttc accgtctacc gctttgataa cgccctgcca tttacgacgg
    27121 ttttgtaccg ccatacggag aaccagagtc acctcttctc cgacaaaacg ggcgtagtgt
    27181 tcagccgtga acagtgggcg atcgagaccc ggtgaggaga cttccaggtt ataagcaacg
    27241 gtgatgggat cttcaacatc cagcacagca cttacctggt ggctcacatc agcacaatca
    27301 tcaacattga tgccatcttc actatcaata tagatgcgca gtgtggatgt gcgaccgcga
    27361 ataaattcga tgcctaccag ttcaaaaccc agggcctcaa ctggcgcagt aatcatctct
    27421 gttaattttt gctctaatgt ggacaagccc acccccaaga cataaaaaaa gggcctaaag
    27481 cccagttatt ctgtagtcag ataacaaaaa accccgataa atcggggctt tatataactg
    27541 aaccctataa ccgcaactgc ggtctggagc actttccaga aggatttttt caaatcccac
    27601 tacgaaggcc gaagtcttca cagtatattt gaaaaaggac tctaagggaa agtggttgcg
    27661 ggggccggat ttgaaccgac gaccttcggg ttatgagccc gacgagctac caggctgctc
    27721 caccccgcgc ctgaaacgtg gcaaattcta ctcgttttgg gtaaaaaatg caaatactgc
    27781 tgggatttgg tgtaccgaga cgggacgtaa aatctgcagg cattatagtg atccatgcca
    27841 cattttgtca acgtttattg ctaatcacgt gaatgaatat ccagttcact ttcatttgtt
    27901 gaatactttt gccttcccct gctctccctt aagcgcatta ttttacaaaa aacacactaa
    27961 actcttcctg tctccgataa aagatgatta aatgaaaact catttatttt gcataaaaat
    28021 tcagtgagag cggaaatcca ggctcatcat cagttaatta agcagggtgt tattttatga
    28081 cgacgattct caagcatctc ccggtaggtc aacgtattgg tatcgctttt tccggcggtc
    28141 tggacaccag tgccgcactg ctgtggatgc gacaaaaggg agcggttcct tatgcatata
    28201 ctgcaaacct gggccagcca gacgaagagg attatgatgc gatccctcgt cgtgccatgg
    28261 aatacggcgc ggagaacgca cgtctgatcg actgccgcaa acaactggtg gccgaaggta
    28321 ttgccgctat tcagtgtggc gcatttcata acaccaccgg cggcctgacc tatttcaaca
    28381 cgacgccgct gggccgcgcc gtgactggta ccatgctggt tgctgcgatg aaagaagatg
    28441 gcgtgaatat ctggggtgac ggtagcacct ataaaggaaa cgatatcgaa cgtttctatc
    28501 gttatggtct gctgaccaat gctgaactgc agatttacaa accgtggctt gatactgact
    28561 ttattgatga actgggcggc cgtcatgaga tgtctgaatt tatgattgcc tgcggtttcg
    28621 actacaaaat gtctgtcgaa aaagcctact ccacagactc caacatgctt ggtgcaacgc
    28681 atgaagcgaa ggatctggaa tacctcaact ccagcgtcaa aatcgtcaac ccgattatgg
    28741 gcgtgaaatt ctgggatgag agcgtgaaga tcccggcaga agaagtcaca gtacgctttg
    28801 agcaaggtca tccggtggcg ctgaacggta aaacctttag cgacgacgta gaaatgatgc
    28861 tggaagctaa ccgcatcggc ggtcgtcacg gcctgggcat gagcgaccag attgagaacc
    28921 gtatcatcga agcgaaaagc cgtggtattt acgaagctcc ggggatggca ctgctgcaca
    28981 ttgcgtatga acgtctgttg accggtattc acaacgaaga caccattgag cagtatcacg
    29041 cgcatggtcg tcagttgggc cgtctgctgt accaggggcg ttggtttgac tcccaggcgc
    29101 tgatgctgcg tgactctctg caacgctggg ttgccagcca gatcactggt gaagttaccc
    29161 tggagctgcg ccgtgggaac gattattcaa tcctgaatac cgtctcagag aacctgacct
    29221 acaagccaga gcgtctgacg atggaaaaag gcgactcggt gttctcgcca gatgatcgta
    29281 ttggtcaatt gaccatgcgt aatctggata tcactgatac ccgagagaaa ctttttggtt
    29341 atgccaaaac tggcctgctt tcctcctctg ccacttcagg cctgccgcag gtggagaata
    29401 tggaaaacaa aggccagtaa ttcgacatca accctgccct tcaggggtgg ggttaccttg
    29461 cttatcctct gtaaactcct ttctgagcat cacataatcg aaaacatcat ggtttgcatc
    29521 cacaacccgt gagtctcctt tatagtgtgc aatcacttcc tcaagcgttt gcggctgttc
    29581 cggctttgtt acccccatcc acgcattaat aaggtaatta ttgtaagctg tcgaaaagat
    29641 attgttttcc gttgtgcgat ccacgccatc cccaagaacg gggctatacc agataaacat
    29701 cgggacatga tatgcctgct ggctagcctc cctgcctcca tgaaaataga cgttcttctt
    29761 cgtagggtca cgttccaggc cgtggtcggc aaaatacatg accgaggcgc ggcgatcttt
    29821 taataattca aaaacctgac ctagcaaact atctgtgtaa tgaatggagt tgtcatagca
    29881 ggcatcctga tcgtcctgcg gttgaaacac ggcgctggat tgcggatagg cgctacaagc
    29941 cggttcatgg cttccattta aatgaagaac aatcagtttc ttttgctgcg tattttgctg
    30001 taatgcttgc gataaatgcg gcaacaacaa ttcatcaaat cctctgacat aaactgtttc
    30061 catggcgcgc atggcgatac tggtaactgc tgtaccattc tgccgaaaag cggattgcga
    30121 gcttagccag aaagtctgaa atcctgcttg attagccata ttaataatgt tgtcggggta
    30181 attatgaatg tcatgactca aaacagaatc agcagttaaa gaaaggggaa ccgacagcgc
    30241 agtgtaaggt gcgccgctta ttgcttgatt aaacagtttg atctgttttc tttgtgcttc
    30301 aacttgcggt gtcgtagagc gtgtatatcc atacaaagac atattgtcga cacgtacaga
    30361 ctcccccaca atcaacacgt aggtatcaat acttgtatcc ctgactgata attgaaaata
    30421 cggcaccgtg tttgcaattg agagtaatct ttgatgcgct ttcgctgcta aagcaaaata
    30481 gttgagattg aaaaacggcg tataggtagc aaatcacgac gctagtatat atggactgaa
    30541 cgctttttta ttttttgcat ctttataagc aaattgacaa gcggaaaata aactgcccga
    30601 aatgacaatc agcaataata ttcctgtcac tttttttgtc ggcaaggaaa catcatattt
    30661 tattattact gccaaaaaaa gaagggataa aaaagcaaaa gcacatagat aagggatata
    30721 catccccagc attttcacaa cttcatccgg atcactctgc agcacactaa tcgcaaatcc
    30781 atcattgaaa gttgtaccaa aggtacacca tgaatagagg ctaatactca tgtctgccgc
    30841 agtcagtaac acaaatggtg cggcaacaag caggcgaagc ggaagggatg atatacgttt
    30901 aacaaccagg atggtcagga cgaagaacag caatcgctga accatatgcg ctccggaaga
    30961 ggcgactaag ttcgtaatac caaaaacaat aacacaaaga tacaacaacc aatgagattt
    31021 aaaatcttct agcaaatttg ttgaatactg tcatccagaa atccaagttc acaaccaata
    31081 taaacaatta tcaacaatgt tcaattttat atcagacatg tttttcaact ctgatccata
    31141 tcagaccgaa acactgttag ttttttcttt ttaaatgatg cgaccattgc gttctcagta
    31201 attaaataac attatcaatg cgtctctaaa cattacgcag aacgtagcca tcagtagtta
    31261 tactgacagg agcaggatgg tttatcgaga agtaaacata tccgtattcg taccggaact
    31321 ggagttgaca caataaagtg ccaattatgt cagcagtagg gaaaagttta taactaaggc
    31381 gtaccggcac catcgtttca aggtaccagc tacgagtaaa gcaactggac gagatacaga
    31441 tacctgacaa ccattcatca gaccaggacc aaaacgaaaa gagacgcttt tcagcgtctc
    31501 ttttctggaa tttggtaccg aggacgggac ttgaacccgt aagccctatt gggcactacc
    31561 acctcaaggt agcgtgtcta ccaattccac cacctcggca cggatactac tttttagttc
    31621 gggatatcgc tggtcggctt agccggagca gctggctgag tttgttcggt tttcgccggt
    31681 gcactcagat tttcccattc gctacctcta ttggttttgt tgctattgat gttacccagc
    31741 accagactga tgatgaagaa taacgttgcc agcagcgccg tcatgcgggt catgaagtta
    31801 ccagaaccac ttgaaccaaa cagcgtagcg gaagcgcctg ctccgaagga ggctcccata
    31861 tcagcgcctt taccttgctg cagcatgatc agaccaacaa ggccaattgc cacaataagg
    31921 aaaactacta aaagagcttc gtacataatc aacctgttcc ttgcggagtt gccgcgtacc
    31981 aatgcttcaa ccaataaagc gggagttttt aatctttccc actgaagcgg gtgtgaatac
    32041 taaccaaaac gaatgacctt cgcaagggca attttatcgc attgtatcaa ctgcggaaaa
    32101 aaacagcaaa accgatgtgt tcgccgagaa aaaaggcgac aatcgccgcc tttttagcca
    32161 gttatctaac gctttaaacg gcttttactg catcggcgat gcggtgtgca aattcagtca
    32221 cctgcgcttc gtcttcgcct tccaccatca cgcgaattaa cggttcggtg ccggatttac
    32281 gcagcaacac gcgtccacgg ttgcccagcg cagcttcaac ctctgcggtc acggctttaa
    32341 ctgactcatg ctcaagcgga tcgccgctac ctgcggtgta acgtacgtta accagaatct
    32401 gcgggaacat tttcatgccg ctgcaaaggt cgtgcaggct catatggtta cgtgccatcg
    32461 ccgccagcac ctgcaagcca gcaacgatgc cgtcaccggt agtggtttta tccagcagga
    32521 tcacatgacc ggaattctct gcaccgatac gccagccttt ctcctgcatt ttttccagta
    32581 cgtagcggtc acccactttc gcgcgcgcaa atggaattcc taactgtttc agcgccagtt
    32641 caagccccat gttgctcatc aatgtaccca cagcaccacc acgcagctgg ccctgacgaa
    32701 gaccttcacg cgcgatgata tacatgatct gatcgccatc gactttattg ccttcatggt
    32761 caaccataat cacgcgatcg ccgtcgccgt cgaaggcaat accgagatcc gctttttcag
    32821 ccagcacacg agcctggagc gcgcgaacgt cggtagcccc cacttcggca ttgatgttta
    32881 caccgtttgg ctcacaaccg atagcgataa cgttcgcccc cagttcgcgc agcacgttcg
    32941 gcgcgatgtg ataagtcgca ccgtttgcac aatccaccac aatcttcagt tcactgaggc
    33001 taagttcgtt cgggaacgtg gctttgcaaa actcgatata gcgacccgcg gcatcaacga
    33061 tacggctggc tttacccagt tcagccgaat caacgcagct gatctccttt tccatttccg
    33121 cttcgatggc ctcttctacc gcatccggca gtttagtgcc gtcgatagag aagaatttaa
    33181 tcccattatc gtagaacggg ttatgcgatg cagatatcac aattccggcc tctgcgcgga
    33241 aggtacgcgt cagataagcc acggccggtg ttggcatcgg gccagtgaag agtgcggaaa
    33301 ggcccgctgc cgccagaccc gcttccagtg ctgactccag catatagcca gaaatacgcg
    33361 tgtctttacc aataataatc ttacgggagc cgtggcgcgc cagcacttta cccgcggccc
    33421 aacccagctt aagcacaaaa tcaggtgtga tcggcgcatc ccctacacga ccacgaatcc
    33481 catcggtacc gaaatattta cgattactca tagcgtttgt tttcctttgc agacagagtg
    33541 gcttccacca cccgcatcgc ttctacggtt tctttgacgt catgaacacg aatgatgtgc
    33601 gcgccttgca ttgcggcaat gaccgcacag gccagactac cgctcaggcg ctcggacggc
    33661 cccacgttca gcagctgccc aatcatcgat tttcgtgaca tacccaccaa cagcggcagg
    33721 ttgaaatggt gaaattcagc caggcgcgcc agtaatgaat agttatggga gagattttta
    33781 ccgaaaccga atccggggtc gagcaacaat ttctcttttg cgatacccgc ctgctcgcaa
    33841 cgtgctattt gctcaataaa gtagcgattc acttctgcaa agacatcgtc atacttcgga
    33901 gcttcctgca tggtttttgg atttccctgc atatgcatca gacaaaccgg taaaccggtt
    33961 tctgcagccg cctccagagc gccaggttcg gaaagggagc ggatatcatt aataatgtga
    34021 gcgccaactt tcgctgactc acggatgact tctggtttgg atgtatcgac cgagatccag
    34081 acttcgaagc gttgagcaat tgcctcaacc acaggaataa cacgttgcaa ctcttcttca
    34141 acgctaactt ccgccgcccc tgggcgcgtg gactcgccac caacgtcaat gatcgtcgcg
    34201 ccagcattga tcatcagatt cgcatgtttc accgcatcta tcagcgagtt atgcgtgcca
    34261 ccatccgaaa aggaatcagg cgtgacgttg aggatcccca ttacgtgagg atggctaagg
    34321 tccagtgaag taccctgggc aaagagtttc attatgttat ccctggtatg aattgataag
    34381 aaaaaacccc ggagcacgcc ccggggtttt cggtacaaat acagtcatct gatgcgggaa
    34441 cttacttgtc gcctaactgc tctgacatgg tgttacccgg gttcggcgta cgcggttcat
    34501 caaccggacg aggagccttt ggactaccat tgtcgccaga attgttagaa gcgcctggtt
    34561 cttcccagcc cgctggcgga cgtacatcgc gacgtgccat caggtcatca atctgcggtg
    34621 cgtcgatagt ctcatatttc atgagagcat ctttcatcgc atgcagaata tccatattgt
    34681 cggtcagaag ctgacgcgca cgattatagt tacgctcaat cagtgctttc acttcctggt
    34741 cgatgatacg tgcagtttca tcggacatat gtttcgcttt cgctacgcta cggccgagga
    34801 atacttcacc ttcttcttcc gcgtacagca gtggacccaa tttctcagag aagccccact
    34861 gagtcaccat gttacgtgcc aggttggtcg caactttaat atcgttggac gcaccggtag
    34921 aaacatgttc cggcccgtag atgatctctt ctgccagacg accaccgtac agcgtagaaa
    34981 tctggctttc cagtttctga cggctggcgc tgattgcgtc gccctcaggc aagaagaaag
    35041 tcacacccag cgcacgaccg cgtgggataa tcgtcacttt atgcaccgga tcgtgttccg
    35101 gcaccaggcg accgataatc gcatgacccg cttcgtggta agccgttgat tctttctgcg
    35161 cttccgtcat caccatggag cgacgttccg cacccatcat gattttgtct ttcgctttct
    35221 cgaactcaac catcgacaca acgcgtttgt tgccacgagc agcgaacagt gccgcttcgt
    35281 tcaccaggtt cgccaggtca gcaccggaga aaccaggagt accacgagca atgattgccg
    35341 cgtcgatatc gggtgccaat ggtacgcgac gcatgtgaac tttcaggatc tgctcacgac
    35401 cgcgaacatc tggcaagccg accacaacct ggcggtcgaa acggccagga cgcagcaggg
    35461 ccgggtcgag aacgtccgga cggttagtcg cggcgataac gatgatacct tcgttacctt
    35521 cgaagccatc catctcaacc agcatctggt tcagagtctg ttcacgttca tcgtgaccac
    35581 cgcccagacc agcgccacgc tggcggccta cggcgtcgat ttcatcgata aagatgatgc
    35641 acggtgccgc tttcttcgcc tgttcgaaca tgtcacgaac acgggatgca cccacaccga
    35701 cgaacatttc tacgaagtca gaaccggaga tagtaaagaa cggaactttc gcttcgcctg
    35761 caatcgcttt cgccagcaac gttttaccgg tacccggagg accgaccatc aggacgcctt
    35821 tcgggatctt accgccgagt ttctggaagc ggctcggctc gcgcagatac tctaccagtt
    35881 cagcaacttc ttcttttgct tcgtcgcagc ccgcaacgtc agcaaaggtc gttttgatct
    35941 gatcttccgt cagcatgcgc gctttgctct taccaaacga catggcacct ttgccaccgc
    36001 cgccctgcat ttgacgcatg aagaagatcc agacaccaat cagcaacagc atcgggaacc
    36061 aggagatgaa gatagaagcc agcaggcttg gttcttcagg cggttcaccg acaaccttga
    36121 cgttcttggt caacaggtta tccagtaatt tcggatcctg aaccggaatg taagtggtat
    36181 aacggttact atctttcttg gtaacgttga tttcacgtcc gttgatacgc gcttcacgaa
    36241 cctggtcgtt attgacctct tgtaggaagg tagagtaatc caccttacgg ccattagact
    36301 cgctgggccc aaagctctgg aatactgaca tcagcacaac ggcaatgacc agccagagta
    36361 ttaggttttt cgccatgtca ctcaagggat taacctctta ttacaactgt gttaaaaaca
    36421 gcgtcaggat actctatatc cagcatcttt caaactttcg tctgaaatct cccggttagg
    36481 gtttacgccc ggtcgctaca atatacactt ctcgcgaacg tgcacgagaa gagtccggct
    36541 tacgaacttt gaccttcgta aacagggagc gaatttccct tagatactca tcgaaacctt
    36601 cgccctggaa caccttcact acaaaactgc cacctggcgc taatacatca cgacacattt
    36661 ctagcgccag ttccaccaga tacatggctc gggggatatc caccgccggt gttccgctca
    36721 tgtttggtgc catatcggac atgacaacct ggactttgct gtcgccaacg cgctccagca
    36781 gtgctttcat caccagttca tcacgaaaat cgccctgaag aaagtccaca ccaacgatag
    36841 gatccatagg taaaagatcg caagcgatga tgcggccttt gccgccaatt tgggtgacca
    36901 catattgtga ccaaccaccc ggagcagcac caaggtcgac aaccgtcatt cccggtttaa
    36961 agagtttgtc actttgctgt atttcatcaa gtttaaacca ggcacgggaa cgtaatccct
    37021 ttttctgtgc ctgttgaaca tatttatcgc taaagtgttc ctgaagccag cggctggagc
    37081 tggcagaacg cttcttacct gtcatttaac tttcccatgg ggataactca tcgtaaccaa
    37141 ttgcgtaaat ttttacgcgc ctatttggtg atatatggga gatggcggta gaatgacccg
    37201 ttttcaatcc caacgtaagc aaaaaatacg atgaatctga gtactaaaca aaaacagcac
    37261 ctgaaaggtc tggcacatcc gctcaagcca gttgttctgc ttggcagtaa tggtttgacc
    37321 gaaggggtgc tggccgagat tgaacaagcg ttagagcacc atgaactcat caaggtgaaa
    37381 atcgccaccg aagatcgcga aacaaaaacc ttgatcgtgg aagctatcgt gcgcgaaacc
    37441 ggcgcctgta atgtacaggt catcggtaaa acgctggtgc tttatcgccc aactaaagaa
    37501 cgtaaaatct cgctgccacg ctaagattat cctaaagtta cacacatttg ctgtgtaaaa
    37561 cgaggggttt tccgcaggca ggagagcaaa atgccacgct ctgttcgttg ataaaaggcc
    37621 gcagagcggc ctttttcctt tctttacaat acatcaacat cttgagtatt gggtaattct
    37681 tacaggtatt ccaccttaat tacttcaaat tctacttcgc cgcccggcgt tttgatgacc
    37741 acaacatcat cttcttcttt gccgatcagg ccacgagcaa taggcgagtt tacagaaatc
    37801 aggttttgtt taaagtcagc ttcgtcatcg ccaacgatgc gataagtctg ttcttcgtca
    37861 gaatccagat tcagcaccgt tacggtagca ccaaaaataa cgcgcccatt gttgggcatt
    37921 ttggtgacat caatcacctg cgcgtttgac agcttggctt cgatgtcttt gatacggcct
    37981 tcgcagaagc cctgctgttc acgagctgcg tggtattcgg cgttttcttt caggtcgcca
    38041 tgctcacgcg cttccgcgat agcagcaatg atttcaggac ggcgcacaga tttcagaaaa
    38101 tccagctctt cgcgtaattt ttcagcgccg cgtaaggtca tcggaatagc ttgcatttgt
    38161 tatacctctt gaatattcct gatggggcaa gtcttcaccc accccggctg ttcggcctgc
    38221 ccggcataac tcctcaccgg gaccagaagc aaaaaaatac cgacccgggt acaagtccca
    38281 ggtcagctac aattcacatt ttgatggtca ttttaccctg aagttcccga agggtcatcg
    38341 tttactttat agggcgttgc gccgtagtat gacggctcga ttccaggttg ttagcgcgag
    38401 attatgcgat tttccagatt tatcatcgga ttgaccagct gtatagcgtt cagtgttcag
    38461 gccgcgaatg ttgatgagta cgttactcaa ctccccgcag gtgccaacct tgccctgatg
    38521 gtgcaaaaag tcggtgcgtc ggcccccgct attgattacc acagtcagca gatggcgctg
    38581 cccgccagta cccagaaagt gattactgcg ctggcggcgt tgattcaact cggccccgat
    38641 tttcgtttca ccacgacgct tgaaaccaaa ggcaatgtgg aaaacggcgt acttaagggt
    38701 gacttagtgg cgcgatttgg tgccgatccg acgttaaaac gtcaggatat tcgcaatatg
    38761 gtcgcgactt tgaaaaaatc tggcgtcaac caaatcgatg gcaatgtgtt gatagatacc
    38821 tccattttcg ccagccacga taaagccccc ggctggccat ggaatgacat gacacaatgc
    38881 tttagcgctc cgcctgctgc cgccatagtt gaccgcaact gtttctccgt ctcgctctac
    38941 agtgccccaa agcctggtga tatggctttt atacgcgtgg catcttatta ccccgttacg
    39001 atgttcagcc aggtacgcac cctcccccgt ggttctgccg aagcgcaata ctgcgaactg
    39061 gatgtggtgc caagcgacct gaaccgcttt acgctgacgg gatgcctgcc acaacgttct
    39121 gagccgctcc cgttggcttt tgccgtgcag gatggagcca gctatgccgg tgcaattctg
    39181 aaagatgagt taaaacaggc gggtatcacc tggagcggaa cactgctgcg ccagactcag
    39241 gttaacgaac ctggaacggt agttgccagt aaacagtcgg ccccgctgca cgatctgctt
    39301 aagattatgc tgaaaaagtc ggacaacatg atcgccgata cggttttccg catgatcggc
    39361 catgcgcgct tcaatgtgcc tggaacatgg cgggctgggt cggacgccgt gcgtcagatc
    39421 ctgcgccagc aagccggtgt cgatattggt aacaccatta ttgccgatgg ttcagggctt
    39481 tcgcggcata acctgattgc ccccgccacc atgatgcagg tgctgcaata cattgcccaa
    39541 cacgacaatg aacttaactt tatctccatg ctgccgcttg cgggctatga cggctctttg
    39601 cagtaccgtg caggtctgca tcaggcgggc gtggatggca aagtctcagc gaaaaccggt
    39661 tcgttgcagg gggtatataa cctggcggga ttcattacca cagcgagcgg gcaacgaatg
    39721 gcatttgtac aatatctttc tggctatgca gtaaaacccg cggatcagcg taatcgccgt
    39781 attccgttag tgcgttttga aagccgtttg tacaaagata tttatcagaa caattagtca
    39841 aaaagaaacc ccggcgcatg gccggggttt cagattattg acaaagtgcg ctttgtttat
    39901 gccggatgcg gcgtgaacgc cttatccggc ctacaaaatc gtgcaaattc aatatattgc
    39961 aatcatcttg taggcctgat aagtgtagcg catcaggctt atttggcatt ggtcattagt
    40021 gattaacgct tgtaaatgaa ctcaacgcct tcttcgtcgt cttcgtccca gtcgtcatcc
    40081 cagtcttcat catcctcttc agcaatctct tcaagctgct gacggtgata atcatcccac
    40141 atgaattcga ctttctctgg ctgtttcgct tcttcagcct gcacgaccgg gttttcaatg
    40201 ataaaggtca tcacatccca gcagagatct ttcacgccca gtccgctcgc cgcagagatc
    40261 agataatatt tatcttccca gcccagcgct tcagcgatcg ctttcgcttt ctcttcggct
    40321 tctgccttat ccagcagatc gatcttgttg aagactaacc aacgcggttt cgccgccaga
    40381 tcctggctgt atttttccag ctcgctgata ataatacgcg cgttttcaac cggatcggtg
    40441 ccgtcaatcg gatcgatatc gatgaggtgc aacaggacgc ggcaacgttc caggtgcttc
    40501 aggaagcgaa tgcccagacc tgcgccttcc gcagcgcctt caatcagtcc tggaatatcg
    40561 gcaacaacga agctcttttc gttgtccatt cgtaccacac ccagacttgg caccagagtg
    40621 gtaaacggat aatccgccac tttcggttta gccgccgata ccgcacgaat aaaggtcgat
    40681 ttacccgcgt ttggcatccc caacataccg acgtcagcca gcagcatcag ctccagcagc
    40741 agctcacgct tatcgcccgg cgtaccgtta gttttctgcc gtggtgtacg gttaactgac
    40801 gatttgaaac gggtattgcc cagaccgtgc cagccgccct tagcaaccag cagacgctga
    40861 ccgtgtttag tcatatcacc catggtttca ccagtaccct ggtcgattac acgcgtacct
    40921 accggcactt taatcgtcac gtctttaccg cgcttaccgg tacaatcgcg gcttgcgccg
    40981 ttctgaccgc gctctgcacg gaaagatttt tcaaaacgat aatcgataag cgtgttcagg
    41041 ttctcgtcgg cttccatcca tacgtcacca ccatcaccgc cgtcgccgcc atccgggccg
    41101 cctttcggaa tatacttttc gcggcggaag ctcacgcaac cattaccgcc atcacctgca
    41161 acgaccagaa tcgatgcttc atcaacaaac ttcattttat tctccgtaaa tcattcgcct
    41221 gcgcggggtt gcgataccac cgttgtatgc ttacgtaatc cgccccaaat acgatgacca
    41281 atggcggaat acatcgcgcc cgcaaccacg acaaacgcac cgagataacc taaaaggttt
    41341 aacatcggtc tggcgaagaa atcgggccag gccagtgata aaagatctga aaaaaacagc
    41401 gtaaacagtg gggtgagcgt gatgatcgcg ctcacctgcg ctgcctgcca gcgagccatc
    41461 gcttccgcca aggcgccata tcctaccaag gtattcagtc cgcaaaaaat taaacatgcg
    41521 agctgccagt ggctaagctg cgctatcact ccaggctttg ccagagggaa gagcgcgatt
    41581 gtacataaag tgtacagtaa aaacaggatc tgcggtgagg ccagccgacg caataaaacc
    41641 ttttgcgcca cgccataact cacccaaacc gtcgccgcac cgaccccaaa gataactccc
    41701 caggtgtaat cggtgagctt tgtaaatatc tcgaccagac tggtgttaaa aaacatcacc
    41761 agaccgctca ggagcatcaa tgcccctaca acctgagtgc tgcgcatttt ctctttcagg
    41821 ataaatacgc tggcaaccat catgccaact ggcgagagtt gcccaatcac ctgcgaagcg
    41881 gtcggactca ggtattgcaa ggatgagctg aacaggatga agttcccaaa cagcccggcg
    41941 gtcgccactg ccaacaaaat caaccagcgt ggcttacgaa acacgcgtaa tggcggcaac
    42001 ctcttcttca ccgcaagaat ggcaccaagg ccaatactcg ccatcaagaa acggtaaaac
    42061 acgattgtcg gaggttccat cacctccagc acctgcttca ttgcgattgg caacgccccc
    42121 cagcaaattg ctgtggtaag cgccaaaaga atgccaatgc ctgcctgctg cttcataccg
    42181 tattccctgc aaagcgcatt tgcggattaa cgtcgcacca tgcgcatttt taccggttat
    42241 cgaatgtaaa aagccccgca acgtgttgcg gggctttcat ccgttaccgg gacgcgaaaa
    42301 acttattcag cttcgatgct gataaattta cggtttttcg ggcctttaac ttcgaatttc
    42361 actttaccgt ctgctttagc aaacagagtg tggtcacgac cgcaacctac gttagcgcca
    42421 gcgtggaatt tggtaccacg ttgacgaacg atgatgctac ccgccagaac ggattcgcca
    42481 ccgaaacgct taacgcccaa gcgtttagct tctgaatcgc gaccgttacg tgtggagccg
    42541 ccagcctttt tatgtgccat ttgaaatctc tcctcaggtc ttaggcgctg atgccagtaa
    42601 ttttcacatc agtgaaccac tgacgatggc cctgctgctt acgatagtgt ttacgacgac
    42661 gaaacttaac gattttaact ttctcgccac gaccgtgagc aacaacttca gctttgatta
    42721 cgccgccatc aacgaaagga acgccgattt tgacttcttc accgtttgcg atcatcagca
    42781 cttcagcgaa ctcaacagtt tcgccagttg cgatgtccag cttttccagg cgaacggtct
    42841 gaccttcgct tactcggtgt tgtttaccac cactttggaa aaccgcgtac atataaaact
    42901 ccgcttccgc gcacaccttt tcaatgattc agagtgcgct ataaatattc acaatagggc
    42961 gcgaatatta cgcaaaacgc acgcctttga caagtgctac agtcaataca cgaagaaaaa
    43021 aacacaactt gtacggtaac gtttatctgt gccatttttt cagtacaatt accctatatt
    43081 cctaaccata aaccctaagt tgcctttgtt cacagtaagg taatcggggc gaaaagcccg
    43141 gcttttgcga tgaatttaga aaaaatcaat gagttaaccg cgcaagatat ggcgggtgtt
    43201 aatgcggcaa tccttgagca gcttaattcc gacgtccaac tgatcaatca gttaggctat
    43261 tacatcgtca gcggcggcgg taaacgtatt cgtccgatga ttgctgtact ggctgcacga
    43321 gctgttggct atgagggaaa tgcgcatgtc accattgctg ccctgatcga gtttatccac
    43381 acggcgactc tgctacacga cgacgttgtg gatgaatcag atatgcgcag gggtaaagct
    43441 accgccaacg ccgcatttgg caatgccgcc agcgtgctgg taggcgattt tatttatacc
    43501 cgcgcgttcc agatgatgac cagcctcggt tcactcaaag tgctggaagt catgtcagaa
    43561 gctgtaaacg tcatcgcaga aggtgaagtt ctgcaactga tgaacgttaa cgatccggac
    43621 atcactgaag aaaactacat gcgcgttatc tatagcaaaa ccgcgcgtct gtttgaggct
    43681 gccgcgcagt gttccgggat tctggctggc tgtacgccgg aggaggagaa aggcctgcag
    43741 gattatgggc gctatctcgg cactgctttc cagttgatcg acgatttact cgattacaat
    43801 gccgatggcg aacagttagg taaaaacgtc ggcgacgatc tgaacgaagg taaaccgacg
    43861 ctgccgctgc tgcatgcgat gcatcatggc acaccagaac aggcacagat gatccgtacc
    43921 gccatcgaac aaggtaacgg tcgccatctt ctggaaccgg ttctggaagc aatgaacgct
    43981 tgtggatctc ttgaatggac gcgtcagcgc gccgaggaag aagcagacaa agccatcgca
    44041 gcgttacagg tgctcccgga caccccttgg cgagaagcac tcatcggcct cgcgcacatc
    44101 gctgttcaac gcgatcgtta atcccctccc ttcatcccgc gcagtgcgcg ggatgattcc
    44161 agtaaattcc ataaaaacac taattcagcg ctataacacc tgaaaaaagg gcgatgaatc
    44221 tttatatatt ctgaatattc acactcttta caggaacttt ttagcgcaat agaccatcag
    44281 gagtatagtg atgcccgaca gaagaagtgt tctgaatgaa agcgactaac ttaaggagtg
    44341 aggaaagatg gaaagtaatt tcattgactg gcatcccgct gacatcattg caggtttgcg
    44401 caagaaagga acgtcaatgg cggcggaatc tcgcagaaat ggtttgagtt cctcaacgct
    44461 ggcaaacgca ttatcgcgcc catggccgaa aggagagatg attattgcga aagccctggg
    44521 aactgacccc tgggttatct ggccatcacg ctaccatgat ccgcagaccc atgagtttat
    44581 cgacagaacg cagttgatgc gtagctacac taaaccgaaa aaatgagtgg tctggcggta
    44641 gccccgcgaa cggggctgcc agctctcaga cgattactcg cctttcacac gctcaatatt
    44701 tgcacctaaa gcgcgcagtt tgtcttcaat gcgttcgtag ccacgatcga tgtgataaat
    44761 acgatcaacc accgtcgtcc cttccgcaat acagccagcc agcaccaggc ttgctgatgc
    44821 acgcagatcg gttgccataa cctgtgcacc agaaagtttt tcaacaccat gacaaataac
    44881 ggtattgctt tcgatttcgg cgtgcgcgcc catacggctc agctctggca catgcataaa
    44941 gcggttttca aagaccgttt cggtgatgaa cccggtccct tcagccacca ggttcaacag
    45001 cgtgaactgg gcctgcatat cggtcgggaa tgccggatgc ggcgcggtac gtacgttaac
    45061 agccttcgga cgtttgccat gcatatccag gctaatccag tcttcgccga cttcgatgtc
    45121 cgctccagca tcacgcagtt tcgccagcac ggcgtccaga gtatctggct gcgcgttacg
    45181 gcagataatt ttgccacgag agatcgccgc cgccaccagg aaagtaccgg tttcgatacg
    45241 atccggcagc acgcgataga caccgccgcc taaacgttcc acaccttcga tgacgatacg
    45301 atcggtgccc tgaccgctaa ttttcgcacc cagcgtaatc aggaagttcg cggtatcgac
    45361 gatttccggt tcacgcgctg cgttttcaat aatcgtggtg ccttccgcca gggttgcagc
    45421 acacatgatg gtcaccgttg cgccaacgct gactttatcc atcacgatat gtgcgccttt
    45481 cagacgacca tcgacggaag ctttaacgta accttcttcc agtttgatgg tcgcgcctaa
    45541 ttgttcgagg ccagaaatgt gtagatcaac cggacgcgca ccgatcgtac aaccgccagg
    45601 tagtgaaact tgcccctgac caaagcgcgc taccagcggc cccagcgccc agatagaagc
    45661 acgcatggtt ttaaccagat cgtaaggtgc gcagaataca ttaacgtcgc gggcatcaat
    45721 atgcacagaa ccattacgtt ctactttcgc acccagctgg cttagcagtt tcattgatgt
    45781 atcgacgtct ttcagtttcg ggacgttctg gatctctacc ggttcttccg ccagtagtgc
    45841 ggcaaaaaga ataggcagag cagcattttt agcgccggaa attgtgactt cgccctggag
    45901 cttcgttggc ccctgaacac gaaatttatc catttagttt gttctcagtt aacaattcat
    45961 atccgctacc ggcgaatcgc ccatagctca aaagccgttc agtttgcgat cgcgcgccca
    46021 ctccgcaggg gtatacgctt tgatcgacac agcatgaatg cggttatccg caatatattc
    46081 catcagcggg ccatagaccg tctgctgttt tttaacccga ctcataccgt caaacaactc
    46141 acccacggca ataacctgaa agtggctgcc atcgccggaa acgtggactt cctggaggga
    46201 gagagcgttc atcaacacgc tctgaatttc attattttcc atgggatctt caatcatcag
    46261 ttaataaacc agcgaaacat cttagagcaa agttgcgctg gcataaataa gcaaaaagcc
    46321 tcgctgataa atcagacaag gctcgacttg caggcaggtt tgccggacag gcggttaacg
    46381 ccatatccgg cctgaaaaaa tttaacgagg cagaacatca gcaggcaaat tatacaattt
    46441 cgcgagggta tacactttgt cgtttacccc ctgaagcgtc acgttgttgc cctgcttttt
    46501 cgccagatcg ataagatgga gcagcagtgc cagtcccccc gtatccacgc gggacacacg
    46561 gctaagatcg atgcaggtaa tccccttcac cgcctcctca cgcatttccc aaagcggtag
    46621 cagaacgtcc tgatccagct ctccggataa cgccagcgtg tcacccgtct gcatccagct
    46681 cagtgactcg ctcattattt tttctcttcc agagtgattt tctgttgaga aatcgatttc
    46741 agttgcgcag tcaggccgtc gatacctttg gtacgcagca gcgttcccca ctcgttttgt
    46801 ttggtggtga tcatactgac gccttcagca atcatgtcgt aagcttgcca gtttcccgtc
    46861 tgggagtttt tacgccactg gaagtccaga cgcaccggcg gacggccatt cgggtcaata
    46921 atggtaacgc gaataggcac aatggtttta tcgcccagcg gctgttctgg cgcaatctga
    46981 taggtttgac cgtgatacat cgccagcgcc tgaccgtaag cctgcttcag gtactcacgg
    47041 aaagcggcaa agtaggcttc acgttgagca ggggtcgcac tcttgtaata ctggcccagc
    47101 accagcgcac cggcgtattt cacctgtacg tatggcagca gttcctgatc aacaatggta
    47161 cgcagataat ccgggttggc ccgaatttgc ggttgctcat tcttcaggcg atcgaacgtt
    47221 ttctgcgccg cctcgtccat cagcttataa ggattggtct ggtctgccgc ggttgccgca
    47281 ctcagaggtg caatcaccag caaagcgacc atcattaaac gtttaaacat gcgtcggttc
    47341 tcctgaaatt atttcgttgt acccacaggt tcagtggttt cattattacc tggcgcagca
    47401 gctggcgcat cgccactatt cttattgtca tcgcctttac taccgtaaag gaactgacca
    47461 atgagatctt ccagcaccat cgcagacttg gtgtcctgaa ttgtatcgcc atccttcagg
    47521 atagcagtcc ccagttccgg gtcttcaaaa ccgacgttta atgccagata ttgttctccc
    47581 agcaggccgg aagtacgaat gctcagcgaa ctggtatcag gaatgtggtt ataacgttgt
    47641 tcaatttcca gcgttacgcg cggcagatag gttttcgggt ccagcgtaat atccgccacc
    47701 cggcccacaa caacaccacc aatgctgacc ggagagcgcg ctttcaggcc gccaatgtta
    47761 tcgaacgtcg cataaagcgt gtaggtcgat tcagtacgta tggacgtcac gttcgccgcc
    47821 ttcaggcaaa caaacagcgc cgccagcagt gctgctaata aaaagatacc cacccaaatt
    47881 tcattttttt tcgtttgcat gaactcaatt cccaaacatc aatgcggtca gcacaaaatc
    47941 cagccccaga acagccagag acgagtggac aacggtgcga gtggttgccc ggctaatccc
    48001 ggcagacgtc gggatggcgt cgtagccgtt aaacaacgaa atccacgtca ccgtgatggc
    48061 gaacaccacg ctcttaatca gacagttgac cagatccata cgccagtcga cggcattttg
    48121 cattgccgac cagaagaacc cgctatcaat gcctttccaa ctgacgccga ccagcgatcc
    48181 gccccagatc cccacggcga cgaagataac cgtcaacagt ggtaatgaaa taaccccagc
    48241 ccagaaacgg ggagaaataa cccgacgcag cggatccacc gccatcatct ccatactgga
    48301 gagttgctct gtagcgcgca tcaggccgat ttctgcggtt aacgccgaac cagcacgccc
    48361 ggcaaacaac agcgcggcaa ccaccggccc cagttcacgc agtagcgata acgccaccag
    48421 catacccaga ctggtttccg cgctataagt ggtcagaacc agataacctt gcagccccaa
    48481 caccattccg atgaacacgc cagaaactac aataatcagc atcgacagga cgccgacatt
    48541 atagagctgg cgcaccagca gcggcgcatg tttgcgaaat tccggtttgc cgaccagcgc
    48601 attgaataac attaacccgg cccgcccgaa cgttctcagg gttttaatcc ctttatgtcc
    48661 gagcgacgcc agcgcattta acagcatgag tggcttaact ccctggtaaa agatcagcgt
    48721 gataatcgcc ggcaggatag cggaacggaa caggcccgtc agctatcccg tccagaaact
    48781 gacgtacgcg cggatcagga ttcgtctgca acgcctgggc actgccatga gcgacaattt
    48841 ttttgtccgc caggatccag gcgtgatccg caatacttaa cacttccggc acatcgtgag
    48901 aaaccaccac acaagtcacg cccagcgcgc tgttcagctc agaaatcagc ttcaccagta
    48961 cgcccatggt gatgggatct tgcccaacaa aaggttcatc aaacatgatg agatccggct
    49021 ccagcgcaat cgcacgcgcc agcgctgcac gccgcgccat cccaccggaa agttcagaag
    49081 gcattagttt agccgctcca cgcagcccca cggcctccag cttcatcatc accgtactat
    49141 gcaacaatgg cgcgggaagt tgggtatgtt cgcgcagtgg ataggcgacg ttgtcaaata
    49201 cgttcatatc agtgaacaac gccccggact gaaataacat gctcatccgt ttacgcactg
    49261 tatacaggcg cgaacgagac atcgccggaa tattctcacc atcgaaaagg atctcaccat
    49321 gatctggtgc gatttgcccg ccaatcagac ggagtagcgt cgttttaccg atgcccgatg
    49381 gccccatgat cgccgtgatc ttccctcgcg gcacggtcag ggaaatatta tcgaagatgc
    49441 agcgattgcc acgcgtaaaa ctgacatcgc gcatatcgac taaattcgcc acagactgct
    49501 ccataattca cccttcgtct tgcgttgatt ttctaagcat ggcgctcaat ttaaccttga
    49561 acccaacata tttacagaat attacccgcc gtggttagcg aaagctggca tttgttttac
    49621 tttttagccg cataaagtca aaattaagaa tccgttacgg ctttctgaaa atcttcagcg
    49681 gaccggcgag tatacctgaa gaaaggacgt tagatgcttt tagctacggc actgttaatt
    49741 gttggtttac ttttggtcgt ttacagtgcc gaccgcctgg tttttgccgc gtctattctt
    49801 tgccgaacct ttggcatccc gccgctgatc atcggcatga cggtggtcag tattggtaca
    49861 tcgttaccag aaatcatcgt ctcgcttgcc gcgtctctgc acgaacaacg cgatttagcc
    49921 gttggtacag ccctcggctc aaacattatc aatatattgc tgatcctcgg tctggctgcg
    49981 ctggttcgtc cttttaccgt ccattccgat gttctgcgcc gtgaattacc cttaatgttg
    50041 ttggtcagcg tggtggccgg ttccgtactc tatgacggac aacttagtcg aagcgatggt
    50101 atctttctcc tctttctggc tgtgctatgg ctgctgttca ttgttaaact tgcacgtcag
    50161 gggactgaca gcctgaccag agagcagctt gcagagctgc cgcgtgacgg cggattgccc
    50221 gtcgcgtttt tatggctcgg cattgcgctt atcatcatgc cagtggccac gcggatggtg
    50281 gttgataacg ccacggtgct ggcgaattac tttgccatca gcgagttgac gatggctctg
    50341 acggcaattg ctatcggaac cagtctgccg gaactggcaa ccgcaatagc gggggttcgc
    50401 aaaggtgaaa acgacattgc tgtcggaaat atcattggcg caaacatttt taatattgtc
    50461 atcgtgttgg gtttacccgc gctgataacg ccaggagaga ttgatccact ggcgtacagt
    50521 cgtgactaca gcgtgatgtt gctggtgagc attatttttg cgttgctgtg ctggcggcgc
    50581 tccccgcaac cgggccgtgg tgtaggggta ttattaactg gcggatttat cgtatggctg
    50641 gcgatgttgt actggttatc gccaatactc gttgaataac tggaaacgca ttatgtcgca
    50701 cgtagagtta caaccgggtt ttgactttca gcaagcaggt aaagaagtcc tggcgattga
    50761 acgtgaatgc ctggcggagc ttgatcaata catcaatcag aatttcacgc ttgcctgtga
    50821 aaagatgttc tggtgtaaag ggaaagttgt cgtcatgggg atggggaaat cggggcacat
    50881 cgggcgcaaa atggccgcaa cgtttgccag caccggtaca ccttcatttt tcgtccatcc
    50941 tagtgaagcc gcgcatggtg atttaggcat ggtcacccca caggatgtgg tgattgctat
    51001 ctctaactca ggtgaatcca gcgaaatcac ggccttaatt ccagtgctta agcgtcttca
    51061 cgtaccgtta atctgcatca ccggtcgccc ggagagcagc atggcgcgcg ccgcagatgt
    51121 gcatctgtgt gttaaagtag cgaaagaagc ctgtccgtta gggctggcac cgaccagcag
    51181 caccaccgcc acgctggtta tgggcgatgc cctcgctgtc gcgctgttaa aagcacgcgg
    51241 ctttactgct gaagattttg cgctctcaca cccaggcggc gcactgggtc gtaaacttct
    51301 gctgcgcgta aacgatatta tgcatacggg cgatgagatc ccgcatgtta agaaaacggc
    51361 cagtctgcgt gacgcattgc tggaagttac ccgcaaaaat cttggtatga ctgtcatttg
    51421 cgatgacaat atgatgattg aaggcatctt taccgacggt gatttacgcc gtgtcttcga
    51481 tatgggcgtg gatgttcgtc agttaagtat tgccgatgtg atgacgccgg ggggaatacg
    51541 tgtgcgccct ggcattctgg ccgttgaggc actgaactta atgcagtccc gccatatcac
    51601 ctccgtgatg gttgccgatg gcgaccattt actcggtgtg ttacatatgc atgatttact
    51661 gcgtgcaggc gtagtgtaaa gattcaagga taaacaacaa tgagcaaagc aggtgcgtcg
    51721 cttgcgacct gttacggccc tgtcagcgcc gacgttatgg caaaagcaga gaacattcgt
    51781 ctgctgatcc tcgacgttga tggcgtactg tcagatggcc tgatttatat gggcaataac
    51841 ggcgaagagc tgaaagcgtt caatgttcgt gacggttatg gcattcgttg tgcgctcacc
    51901 tctgatattg aagtcgctat cattaccggg cgaaaggcta aactggtaga agatcgttgt
    51961 gccacattgg ggatcactca cttgtatcag gggcagtcaa acaaactgat cgcctttagc
    52021 gatttgctgg aaaaattggc gattgccccg gaaaatgtgg cttatgttgg cgatgatctc
    52081 atcgactggc cggtaatgga aaaagtgggt ttaagcgtcg ccgtggccga tgcgcatcca
    52141 ctgttgatcc cgcgcgccga ttacgtgacg cgcattgctg gcggtcgtgg cgcagtgcgc
    52201 gaagtttgcg acttattact cctggcgcag ggcaaactgg atgaagccaa agggcaatcg
    52261 atatgagtaa agccagacgt tgggttatca ttgtgctatc actggcggtt ctggtgatga
    52321 tcggcattaa tatggccgaa aaagacgata ccgcccaggt ggtcgtcaac aacaatgatc
    52381 ccacctataa aagcgagcat acggacacgc tcgtctataa cccagaaggg gcactaagct
    52441 atcgattgat tgctcaacac gttgaatatt attccgatca ggccgtttcg tggtttacgc
    52501 agccggtact taccacgttt gataaggata aaatcccgac atggtccgta aaagcagata
    52561 aagccaagct gaccaatgac cggatgctct atttatatgg acacgttgaa gtcaacgcac
    52621 ttgtgccaga ctctcaactt cgcagaatca cgacggataa cgcgcagatc aatctggtga
    52681 cgcaggatgt tacctctgaa gacctcgtca cgttatacgg aacaacattt aactccagcg
    52741 gtctgaaaat gcgcggcaac ttacgcagca agaacgccga gctgattgaa aaggttagaa
    52801 catcctatga aattcaaaac aaacaaactc agccttaatc ttgtgcttgc cagctcactt
    52861 ctggccgcca gcattccggc atttgccgta accggagaca ctgatcagcc gatccacatt
    52921 gaatcggacc agcaatctct tgatatgcaa ggcaacgtgg ttacctttac cggtaatgtc
    52981 atcgtcaccc agggcaccat caaaattaat gccgacaaag tggtcgttac ccgtccgggc
    53041 ggcgaacaag gtaaagaagt gattgacgga tacggtaaac cggcaacgtt ctaccagatg
    53101 caggacaacg gtaaacccgt tgaaggtcac gcttcccaga tgcactacga actggcaaaa
    53161 gattttgtcg ttctgacggg taatgcttat ctgcagcagg tcgatagcaa cattaagggc
    53221 gataagatca cttacctggt gaaagagcag aaaatgcagg ctttcagcga caaaggcaag
    53281 cgcgtaacca ccgttctggt gccgtcgcag ctgcaggaca aaaacaacaa aggccagacc
    53341 ccggcacaga agaagggtaa ttaattcgtt atggcaacat taactgcaaa gaaccttgca
    53401 aaagcctata aaggccgtcg cgtggtagaa gacgtcagcc tgaccgtcaa ctccggggaa
    53461 attgtcggtc tgctggggcc aaacggtgcc ggtaagacca ccactttcta catggttgta
    53521 ggcattgtgc cgcgcgatgc gggcaacatc attattgatg atgacgatat cagtctgctg
    53581 cctctgcatg cacgcgcgcg ccgcggtatc ggctatctgc cacaggaagc ctccattttc
    53641 cgtcgcctca gcgtttacga taacctgatg gcggtactgc aaattcgtga cgacttgtct
    53701 gctgaacaac gtgaagaccg cgcgaacgag ctgatggaag agtttcacat tgagcacctg
    53761 cgtgacagca tggggcagtc actctccggg ggtgaacgtc gccgtgtaga aattgcccgc
    53821 gcactggctg cgaatccgaa atttattctg ctcgacgaac cgtttgccgg ggttgacccg
    53881 atctcggtta tcgacattaa acgcatcatt gaacacctgc gcgacagcgg cctgggcgtg
    53941 ctgatcactg accacaacgt gcgtgaaaca ctggcggttt gtgaacgcgc ttatatcgtc
    54001 agtcaggggc atttgatcgc ccacggcacg cctacagaaa tcttacaaga cgaacacgtt
    54061 aagcgtgtat accttgggga agacttcaga ctctgatagg gtagaagttt gcgacgtttt
    54121 agcaggagag tacgattctg aacatgaagc aaggtttgca actcaggctt agccaacaac
    54181 tggcgatgac gccacagctc caacaggcaa ttcgtctgtt gcagttgtcg acgctggaac
    54241 ttcagcagga gctacagcag gcgctggaga gtaatccgct gcttgagcaa atcgacactc
    54301 atgaagaaat cgacacccgc gaaacgcaag acagtgaaac gctggacacc gccgacgcgc
    54361 tcgaacaaaa agagatgccg gaagagctgc cgctcgatgc cagttgggac accatttaca
    54421 ccgctggtac accatccggc accagcggtg actacattga cgacgagctg ccggtctacc
    54481 agggcgaaac gacgcagacc ttgcaggatt acctgatgtg gcaggtcgag ctgacaccgt
    54541 tttccgacac tgaccgcgct attgctacct ctatcgtcga tgccgttgat gaaaccggtt
    54601 atctgactgt cccgctggaa gatattctcg aaagtatggg cgatgaagag atcgacatcg
    54661 acgaggttga agccgtcctt aagcggatcc aacggtttga cccggtcggt gtagcggcaa
    54721 aagatctgcg tgactgcctg ctaatccaac tctcccaatt cgataagacc acgccgtggc
    54781 tggaagaggc cagattgatc attagcgatc atctcgatct gttagccaat cacgacttcc
    54841 gcactttaat gcgcgtcacg cgtctgaaag aagatgtgct gaaagaagcc gtcaatctga
    54901 tccagtcgct cgatccgcgc cccgggcagt cgatccagac tggcgaacct gagtatgtca
    54961 ttccagatgt gctggtgcgt aagcataacg gtcactggac ggtagaactc aacagtgaca
    55021 gcattccgcg tctgcaaatc aaccagcact acgcctcgat gtgcaataac gcgcgcaacg
    55081 atggtgacag ccagtttatc cgcagcaatc tgcaggatgc caaatggttg atcaagagtc
    55141 tggaaagccg taacgatacg ctactgcgcg tgagtcgctg tatcgttgaa cagcagcaag
    55201 ccttctttga gcaaggtgaa gaatatatga aaccgatggt actggccgat atcgcccagg
    55261 ctgtcgaaat gcatgaatcg acgatatctc gcgtgaccac gcaaaaatac ctgcatagtc
    55321 cacgaggcat ttttgaactg aagtatttct tttccagtca cgtcaatacc gagggcggcg
    55381 gcgaagcttc ctccacggcg attcgtgcac tggtgaagaa attaatcgcg gcggaaaacc
    55441 cagcgaaacc gttgagcgac agcaagttaa cctctttgct gtcggaacaa ggtatcatgg
    55501 tggcacgccg cactgttgcg aagtaccgag agtctttatc cattccgccg tcaaaccagc
    55561 gtaaacagct cgtttgaccc aaccgataag gaagacacta tgcagctcaa cattaccgga
    55621 aataacgtcg agatcaccga ggcactgcgc gaatttgtta cagctaaatt tgccaaactt
    55681 gagcaatatt ttgaccgaat caaccaggtc tatgttgttc tgaaagtgga gaaagtcacc
    55741 cacacctcag atgcaacact gcatgtaaac ggtggtgaaa ttcatgccag cgcggaaggt
    55801 caggacatgt acgctgccat tgatggctta attgataagc tggcacgcca gttgaccaaa
    55861 cataaagata aactgaaaca acactaattg tccgggcaat tagcatgtgc atggcggcct
    55921 gttgtgcggc acaacgggcc atttgtacgg ttaatgctcc gagcctgttc cactgtttga
    55981 gtgggcaggt tcttaggtga aattatgaca aataatgata caactctaca gcttagcagt
    56041 gttcttaaca gggaatgtac gcgaagccgc gtccactgtc agagcaaaaa acgcgccctg
    56101 gaaatcatca gcgagctggc ggcgaaacaa cttagcctgc cgcctcaggt ggtttttgaa
    56161 gctatcctga cgcgggaaaa aatgggcagt accggtatcg gcaatggtat tgccattccg
    56221 catggcaaac ttgaagaaga tactctgcgt gccgttggcg ttttcgttca gctcgaaacg
    56281 cctatagctt tcgatgccat cgacaaccaa ccggtggact tactttttgc cctgctggtg
    56341 ccggcagacc aaactaaaac gcacctgcat actctgtcgc tggtggcgaa acgtctggcg
    56401 gacaaaacca tctgccgccg tttacgcgca gcccagagcg atgaagagct gtatcaaatc
    56461 attacggata ccgaaggtac tccggatgaa gcgtagttat tcggtaatgt ctcttttaga
    56521 cgttgtgagg agaaacagta catggtactg atgatcgtca gcggacgttc aggttcaggt
    56581 aaatctgtcg ccctgcgtgc gctggaagat atgggttttt actgcgtgga taaccttccc
    56641 gtagtgttgt tacccgatct ggctcgaact ctggccgatc gcgagatttc tgccgccgtc
    56701 agcattgatg ttcgtaatat gccggagtca ccagaaatat tcgaacaggc gatgagtaac
    56761 ctgcctgacg ctttctcacc gcaactactg ttcctggatg ccgaccgtaa taccttaatt
    56821 cgtcgttaca gtgacacgcg ccgactgcat ccgctttcca gcaaaaacct gtcgctggaa
    56881 agtgctatcg acaaagaaag cgatttgctg gagcctctgc gttcgcgagc ggatctgatt
    56941 gtcgatacct cagaaatgtc cgttcacgag ctggctgaaa tgctgcgtac ccgtctgctg
    57001 ggtaaacgtg aacgcgaact gaccatggtc tttgagtctt tcggcttcaa acacggtatc
    57061 cctatcgatg cagattacgt ctttgacgtg cgcttcttgc cgaacccgca ctgggatccg
    57121 aaactgcgtc caatgacagg tcttgataaa cctgtcgccg cgttcctcga ccgccacaca
    57181 gaagtacaca attttatcta ccagacgcga agctatcttg agctatggtt acctatgctg
    57241 gaaaccaaca accgtagcta cctgacggtc gccattggtt gtaccggcgg gaagcaccgt
    57301 tcggtgtata ttgcagagca actggcagac tacttccgct cgcgcggtaa aaacgtccag
    57361 tcacgccatc gtacgctgga aaaacgtaaa ccatgaccgt caagcaaact gttgaaatca
    57421 caaacaagct gggcatgcat gcccggcctg caatgaagct gtttgaatta atgcagggtt
    57481 ttgacgctga agtgctgtta cgtaatgacg aaggcaccga ggctgaagcc aacagcgtta
    57541 ttgcgctgct gatgttggat tctgccaaag gacggcagat tgaagttgaa gcgaccggtc
    57601 cacaggaaga ggaagcactg gccgccgtta tcgccctctt taattctggt tttgatgaag
    57661 attaatcttc atcactttga catacaactc ccttcaagct cccccgacaa taagaaaatc
    57721 acgtactgaa atcgttctca atcaacgtca tttgtacatt ttgtgcgctt ttcactttca
    57781 gaagaacctt aagaaaacct taagaggcat tgtttaggtt ttgtttaagt taatcgacca
    57841 tactggagat cgtcagaaaa tatttccagg agatggcatg attcgcttat ctgaacaaag
    57901 tcccctgggc accggaagac atcggaaatg ttatgcgcat ccggaagatg cccaacgctg
    57961 tatcaagatt gtctaccatc gtggcgatgg tggcgataaa gagatccgcc gtgagttaaa
    58021 gtactacgcg catcttggtc gccggttaaa agactggagt ggaataccgc gctatcacgg
    58081 taccgttgag acggattgcg gaaccggtta tgtctacgat gtgattgccg attttgacgg
    58141 caagccttcc attacgctga ccgaatttgc tgaacaatgc cgttacgaag aagacattgc
    58201 acaactgcgc cagctactga aacagctgaa gcgttatttg caggataacc gtatcgtgac
    58261 gatgtcgctg aagccgcaga atatcctctg tcaccgcatc agtgagtcag aggttatccc
    58321 ggtcgtctgc gacaacattg gtgaaagcac attgattcca ctggcgacct ggtcgaagtg
    58381 gtgttgtctg cgtaagcaag agcggttatg gaaacgattt atcgcccaac cggcgctggc
    58441 tattgccctg cagaaagatc tgcaacctcg tgagagcaaa acgctggccc tcacttcgcg
    58501 cgaagcttaa tccagctggt gttgttgcat aaacggctca ccgcctaact gatacatctg
    58561 ccgtaaaatc cacgcctgac ggctacgcac gtagcccgat ggcgaggaga ctttgaaacg
    58621 aagtggatta ggtaatacag ctgccagtaa tgcagcttcc gaccgggtaa gtttgctcgc
    58681 gggtttgtgg aaataacgtt gtgccgcagc ttcgacgcca aacacaccgt cgccaaattc
    58741 ggcgatattc aggtaaaccg tcaggatacg ctttttgctc cagactgttt ctatccccag
    58801 cgttaatcca gcttccagcc cttttcgtac ccagctacgc ccatcccata aaaagagatt
    58861 ttggccgtct gctgagaaat cgttgaagca ccgcgaatac ggttttcatt gcgctcgttg
    58921 tgcgccaggg ctttctcaat ggaagcgaca tcaaagcccc agtgctcagg aaatttctga
    58981 tcttctgcgg caatcaccgc cagtcccatc cacggcgaga tttgatccat actgacccag
    59041 tcagaatgtg ccacgtaacg aaaattgcca tgcagccagg cgctgacctg tcgctcgacc
    59101 attaccgctg agaagggaac aggcgcaacg ctaaacaacg cgatgccccc gccccagaaa
    59161 acggcgagga caaccattaa ccgcaaaaga aaacggcgaa cgaaactaaa caccgttaag
    59221 cggcttttac tcattcagcc agaaccagca cgcgggaaac cagcttatca atgccgctcg
    59281 ccgcttctgc aatgttctgc gccagcatat atgctggggt ggtgacaatc ttattgtctt
    59341 catcaaccac gatatcatcg acaggacacg gcacatgctc cgcgcccatc tcttccagca
    59401 cttctgcggt atcgatatca gtaccgatgg tcaaacgcag cgggaaatcg aaaattttcg
    59461 gcagcatcgc cggggcaata cacataaaac caagcggttt tccggcctga tgcatcgctt
    59521 gtgccagcgc ctttaattca cggtcaacgg tgcattcgct accaagactg gcaaaattgc
    59581 ttaaattctt cgccgcgcca aacccccccg gcacaatcaa cgcatccagt tcagcggcat
    59641 cggcctgggc cagaggacgg atttcaccgc gcgttattcg tgccgcttca atcagcacat
    59701 tgcgcgtttc cgtcatcgct tcgccagtta aatggttgat aacatcaacc tgctgcttat
    59761 ccggtgcaaa gcagaccgcc tgcgcaccgc tgcgtgaaat agctaacaac gtcaacaccg
    59821 cttcatgaat ttcagaaccg tcatagacgc cgcatccgct cagaattacg ccaattttct
    59881 tcattgtgat catcctttcg caattgactg aaacacatta atttttttaa taaaaatggt
    59941 acgcatcaca catttaactg attcatgtaa caaatcattt aagttttgct atcttaactg
    60001 cgtgcggcct gaaaaacagt gctgtgccct tgtaactcat cataataatt tacggcgcag
    60061 ccaagatttc cctggtgttg gcgcagtatt cgcgcacccc ggtctagccg gggtcatttt
    60121 ttagtggctt ttgccaccca cgctttcagc acttctacgt cgtgacgcca ctcttctttc
    60181 atctcttcaa tccattcacc gacgttatct tcccaggcgg gaaggtcagg agactgaatt
    60241 tgctgaccca gctgttgcag atggcgtaac cccactgacc ccgccgcacc tttaatttta
    60301 tgtccttcct caacaatgcc ttttttatcc tgcgccgtca gattcgactc cagcacgctg
    60361 acatagcccg gcatcatctt ctcaaacacc gctaacccgt cggtgatcag cttcggtcct
    60421 acaagttcga gatactgttc cagcatggga atatcgagca atgcttctga tttactgttc
    60481 tcttctgtcg tcaccgtact ctcctcatca tcctgggtat cccagaattt cttgatcatc
    60541 gcggttagcg ccggaaccga aagcggctta ctcagcacat catccattcc agcattgagg
    60601 tactcttgtt tgtctttcag cacgttagcg gttaaggcca ccagcggcgg taaatcctcg
    60661 cgcggataac gcttcgtcag ttcacgagag atatccagcc cggtcatatc tggcaactga
    60721 atatccagca acaccaggtc gtattcgccc ggtttaaaca tctccagcgc cgccttgccg
    60781 gtcatggcga catcaacgct gttacctaat ttttccagca cagaacgcgc gacaatcacg
    60841 ttcagttcaa tgtcttccac cagcagcaca ttcagcgccg gtaaaggcat atcgtcttca
    60901 tcaaacgcat catcgacctc ttctgccacc gacggtgcgt ggatcgtcaa cgtaaaggtt
    60961 gaacctttgc cctgttcact ggtaaccgta atatcgccgc ccatattttt cgccagacga
    61021 cgagaaacgg ccagaccaat accggtgccg gtggcaggtt taccgccatg actgtctttc
    61081 acctggtaat acatggcgaa gattttatcc agctcatcct gcggaatgcc gataccagaa
    61141 tcttccactt caaaatgcag catatcgcct tcatcgtagc gcacgcgcac ggtaacctgg
    61201 ccttgctggg tgaatttgac ggcgttactg atgaggttcc acaggatctg ccgtaaacgc
    61261 gtcccgtcgg taatgacctg atgcggtaat ggcagcgtag gctccaggtt aaagcgcaat
    61321 cctttttgtt gcgcctgcaa tgcggagaga ttttccagat cggcaaggaa gctggtgaaa
    61381 tcaaccggct gattatcaag ctggaccttg cgccgttcca tcttatccat gtcgataata
    61441 tcgttaaaga tattccccag cgtgacggcc gaaacatgga tagttttgag atatttttcc
    61501 tgctcggcgg tgagttcggt atccagcaga atgcggctca ggccgacgat accgttcagc
    61561 ggtgtacgca attcgtgact gatggtggaa ataaacgtcg ttttgtcgcg gctggcgcgt
    61621 tcaagcgcat cctgataccg cttacgctcg gtaatgtcgc gaccaaagcc catcaaaccg
    61681 tgacgtttac ccacgcggtc gtagtacggc actttacgga tttcaaagca ggctttgcgc
    61741 ccgtccgggt aatccagcca ctgttcatag gtcagtgaca cattatgacg gaacactttt
    61801 tcatcggttt caatgacttt tgcggcggct tccggtgagt aaacatcagc aggtttcagg
    61861 tgaaccagtt gtttttcgct ttttccggtc agcagctcca tcgcgcggtt acagccggaa
    61921 aactctttat cttcgttacg ataaaaaacc aagtcgggtg aagcatcaag gaaggaacgt
    61981 aagaatgagg attgctgctc gagctgaatt tgtgtctctt cgcgctcttt gatttcaatt
    62041 ttcagttggc cgaaggtttc ctgcagttct gcttccgctt tttcacgaac ggcaatttcc
    62101 tgatttagct gggcaatatt atcttttaac tgaacgttga ggctcaaatc gcgctcgcgc
    62161 atctcctcca gtttttgcac cagccgtgac agacgttgtc gtgactcctc cagttgctcg
    62221 acgaccaccg atagaaagta gaccgcccac ggcgtaatca gcaaaccaaa gaagatagaa
    62281 cgaataacat caatgctttc gacctgacca tgcagcacca tggttaccgc catttgtacc
    62341 acaatggcaa gaacgacgag ggccagcgcc agcaacattg agaagcgcac cagacctaac
    62401 ttcatcatca ggtcaacata atactgcgcc agcagacgaa tttgcttcat tagggaattc
    62461 cttcacgaca acctgcccca ataataccca attctgacag ttacgttata aattgtgtga
    62521 gaaatgcgga attcccctca acgacctact ccgttggagg tagccacgga cgtcccagcg
    62581 ccgatccctg tacgccatgt tcattcagat agcgatccag ctcgaccatt cccgtccatc
    62641 gattttcgca ccacagcgga gcaagcagcg tcgggcgacg ggcgctggcg gaaatgcggt
    62701 ggtagatcac ttccggtggc gtatggcgaa tcatttctcc ggcagtgagc gtataatcct
    62761 ccagttcaat accgttcaaa cgccccgctt cccaggcttt cgccataatg ctgcctttca
    62821 caatatgcag cggatgcagc tttatgccgt ccacgctggt ttcaaccact cgctccagcg
    62881 tttgcaaaca ttcagcctgc ccttcgccag gcaggccgac aattaagtgg gaacacacct
    62941 tcagcccgcg ctgacgtgcc agctgggttg tacgctgata acaggcaaaa tcatggccgc
    63001 ggttgatgcg atgcagtgtt ttgtcgtggg cggtttgtag ccccagctcc agccacactt
    63061 cgtaaccctg gtccttatat tcgcaaagca gatccagcac cgcatccggc acgcagtccg
    63121 ggcgggtacc aacacacaaa ccgacaatat tggcctggct caccgcctgc tgatacatag
    63181 aacgcagcac ctgaacttcc gcaaaggtgc tggtatacgc ctgaaagtag gccagatagc
    63241 gtttagcgcg gttaactaaa ttcgcctggt gcgccagttg ctcggcaatg gaacgatgct
    63301 gctgcgcttc atcggcaaac gaggcaacat tacagaatgt gcagccacca cgcccgatgg
    63361 taccgtcacg gttagggcag ctaaaaccgc cgtgcagcgt cagcttatgc accttttgcc
    63421 cataacgacg ggtgagatca ccaccaaaca tattgactaa tttctgtaac tgcataatct
    63481 gatagaccgc gccttgaaaa gaggccaaag cctgccattt ttagctcaat tcggcgatga
    63541 cctggatcaa tcgtcccggc ctgcttttat caactgcata atcaatcaaa attaccgaaa
    63601 tttcatgcac aatcacataa atcacttttg cttatcttgt gtcagatttt tttatctcct
    63661 tatggatttt aggcaaaaac agtagcatga aacgtcatta ccaattaagg cagtataaaa
    63721 tgctggtttt ctcgtcagtt caaggcagga taagggttaa cacgccttta tgacagtcag
    63781 gaattgactg tttctctaac gactctcctt ttagccttaa agataaaatc tattttaatt
    63841 tcagctattt aataaagaat tttgcgctaa agcacatttc tgtaccaata agcttgccat
    63901 ttgacctgta tcagctttcc cgataagttg gaaatccgct ggaagctttc tggatgagca
    63961 gcctgctcat catatttatg cagtaattga gatcccctct tcaccgtatt aaccgatgcg
    64021 aaaaggacaa caagggggcg aatgcgaggc gcgcgtatga cacgcaaacc ccgtcgccac
    64081 gctctttctg tgcccgtgcg cagcggttcg gaagtggggt tcccgcagag cctgggggag
    64141 gttcacgata tgttgtacga taaatccctt gagagggata actgtggttt cggcctgatc
    64201 gcccacatag aaggcgaacc tagccacaag gtagtgcgta ctgcaataca cgcactggcc
    64261 cgcatgcagc accgtggcgc gattctcgcc gatggtaaaa ccggcgacgg ttgcggcttg
    64321 ctgttacaaa aaccggatcg cttttttcgc atcgttgcgc aggagcgcgg ctggcgttta
    64381 gcaaaaaact acgctgtcgg gatgctcttc ctgaataaag atcctgaact cgccgctgcc
    64441 gcacgccgca tcgttgaaga agaactgcaa cgcgaaacct tgtcgattgt gggctggcgt
    64501 gatgtcccca ctaacgaagg cgtgctgggt gaaatcgccc tctcctctct gccacgcatt
    64561 gagcaaattt ttgtgaacgc cccggcaggc tggcgtccac gcgatatgga gcgccgtctg
    64621 tttatcgccc gccgccgcat tgaaaagcgt ctcgaagccg acaaagactt ctacgtctgt
    64681 agcctgtcga atctggtgaa catctataaa ggtctgtgta tgccggcgga tctgccgcgc
    64741 ttttatctgg atcttgcgga cctgcgtctg gaatcggcca tttgcctgtt ccaccagcgc
    64801 ttctccacta acaccgtacc gcgctggccg ctggcgcaac cgttccgcta tctggcgcat
    64861 aacggtgaaa tcaacaccat caccggtaac cgccaatggg cgcgtgcgcg tacctataaa
    64921 ttccagacgc cgctgatccc tgacctgcac gacgccgcgc cattcgtcaa cgaaaccggt
    64981 tctgactcca gttccatgga taacatgctg gaactgctgc tggcaggcgg gatggatatc
    65041 atccgcgcca tgcgcctgtt agtgccaccc gcctggcaga acaacccgga tatggacccg
    65101 gaactgcgtg ccttctttga ctttaactcc atgcatatgg agccgtggga tggtccggcg
    65161 ggcatcgtga tgtccgatgg tcgcttcgct gcctgtaacc tcgaccgtaa cggtctgcgt
    65221 ccggcacgct acgtcattac caaagacaag ctcatcacct gcgcctctga agtcggtatc
    65281 tgggattacc agcctgacga agtggtcgaa aaaggccgcg tcgggccagg cgaactgatg
    65341 gtgatcgaca ctcgcagtgg gcgtattctg cactcggcag aaaccgatga cgatctgaaa
    65401 agccgccacc cttataaaga gtggatggag aaaaacgtcc gccgactggt accgtttgaa
    65461 gatctgcccg atgaagaagt gggtagccgc gaactggacg acgacacgct tgccagctac
    65521 cagaaacagt ttaactacag cgcggaagag ctggactccg taattcgcgt actgggcgaa
    65581 aacggtcagg aagcggtcgg ttcgatgggc gatgataccc cattcgccgt gctctccagt
    65641 cagccgcgca ttatttacga ctacttccgc cagcagtttg cccaggtgac taacccgcca
    65701 atcgacccgc tgcgtgaagc gcatgttatg tcgctcgcca ccagtatcgg tcgtgaaatg
    65761 aacgtctttt gcgaagcaga gggccaggcg caccgtttaa gctttaaatc gccgattctg
    65821 ctctactccg atttcaaaca gctcacgacg atgaaagagg agcactaccg cgcagatacg
    65881 ctggatatca cctttgacgt cactaaaacc acgctcgaag cgacagtcaa agagctatgc
    65941 gacaaagccg aaaaaatggt acgtagcggc accgtgttgc tggtgctctc cgaccggaat
    66001 atcgctaaag atcgcctgcc ggttccggct ccgatggcgg ttggcgcgat ccagacccgt
    66061 ctggtcgatc aaagcctgcg ttgcgatgcc aacatcatcg tcgaaaccgc cagcgcccgc
    66121 gatccgcacc acttcgccgt gttgctgggt ttcggcgcga cggctattta tccgtacctt
    66181 gcctatgaaa cgctgggccg cctggtagac acccacgcga ttgccaaaga ttatcgtacc
    66241 gtgatgctca actaccgtaa cggcatcaac aaaggcttgt acaaaatcat gtccaaaatg
    66301 ggcatctcca ccatcgcctc ttaccgctgc tcgaaactgt ttgaagcggt cggtctacac
    66361 gatgatgtag tgggcctgtg cttccagggc gcggtcagcc gcattggtgg ggcaagcttt
    66421 gaagacttcc agcaggatct gctgaatctg tcgaaacgtg cctggctggc gcgcaagccg
    66481 attagccagg gcggcctgct gaaatacgtc cacggcggcg aataccacgc ctacaacccg
    66541 gacgtggtgc gcacgctgca acaggcggta caaagcggcg agtacagcga ctatcaggaa
    66601 tacgcgaagc tggttaatga gcgtccggca accacgctgc gcgatctgct ggcaattacg
    66661 ccgggtgaaa acgcggtcaa cattgctgat gttgaaccgg caagcgaact gtttaaacgc
    66721 tttgataccg ccgcgatgtc tatcggcgcg ttaagcccgg aagcccacga ggcgctggcg
    66781 gaagcgatga atagcatcgg cggtaactcg aactccggtg aaggcggcga agacccggcg
    66841 cgctatggca ctaacaaagt gtcgcgcatc aagcaggtgg cttctggtcg cttcggcgtt
    66901 actccggcgt atctggtcaa tgccgacgtc attcagatta aagtcgccca gggcgcgaag
    66961 ccgggcgaag gtggtcagtt gccgggcgac aaagtcactc cttacatcgc caaactgcgc
    67021 tattcggtgc ccggagtgac gctgatctcc ccgccgccgc accacgatat ctactctatc
    67081 gaagacttag cgcagcttat tttcgacctc aagcaggtta acccgaaagc gatgatctcc
    67141 gttaagctgg tgtccgaacc aggcgttggc accatcgcga ctggcgtggc gaaagcttat
    67201 gcggacttga tcaccatcgc gggctatgac ggcggcaccg gcgcaagccc gctgtcgtca
    67261 gtgaaatatg caggctgccc gtgggaactc ggtctggtgg aaacccaaca ggcgctggtt
    67321 gccaacggct tgcgtcataa aattcgtttg caggttgatg gcggcctgaa aacgggtgtc
    67381 gatatcatca aagcggcgat tctcggcgca gaaagcttcg gcttcggcac tggcccaatg
    67441 gtggcgctcg gctgtaaata tctgcgtatt tgccacctga acaactgcgc cacgggtgta
    67501 gcaactcagg atgacaaact gcgtaagaat cactatcacg gcctgccgtt caaggtgact
    67561 aattactttg agtttatcgc ccgtgaaacc cgcgagctga tggcacagct gggagtaacg
    67621 cgtctggtgg atctgattgg tcgcaccgac ctgctgaaag agctggacgg tttcaccgcc
    67681 aaacagcaga aactggcgct gtcgaagctg ctggagactg ccgagccaca tgcaggtaag
    67741 tcactctact gcactgaaaa caacccgccg tttgataacg gcctactgaa cgcgcagttg
    67801 ctgcaacagg cgaaaccgtt tgtcgatgaa cgccagagca aaaccttctg gttcgatatc
    67861 cgcaacaccg accgttctgt cggcgcgtcg ctttcaggct atatcgccca gacgcacggc
    67921 gatcaggggc tggcagccga tcctatcaaa gcgtacttca acggcaccgc aggccagagt
    67981 ttcggtgtgt ggaacgcggg cggcgtggaa ctgtacctga ccggggatgc caacgactat
    68041 gtcggtaaag gcatggcggg cggcttaatc gccattcgtc ctccggttgg ttccgccttc
    68101 cgcagccatg aagcaagcat tatcggcaac acctgcctgt atggcgcgac cggtggtcgt
    68161 ctgtatgccg caggccgcgc gggtgaacgt ttcggcgtgc ggaactccgg tgctatcacc
    68221 gtggtagaag gcattggcga caacggctgt gaatatatga cgggcggtat tgtctgtatt
    68281 ctgggtaaaa ccggcgttaa cttcggcgcg ggcatgaccg gcggcttcgc ttacgttctc
    68341 gatgaaagcg gtgatttccg caaacgcgtt aacccggaac tggtcgaagt cttaagcgtc
    68401 gacgatctgg cgatccatga agagcatctg cgcggtctta tcaccgagca tgttcagcat
    68461 accggttctc agcgcggtga agagattctg gcgaactggt caaccttcgc cacgaaattc
    68521 gcgctggtta aaccgaagtc cagtgatgta aaagcactgc tgggtcaccg tagtcgtagc
    68581 gcagcagagt tgcgcgtgca ggcgcagtaa ggggtagcaa caatgagtca gaatgtttat
    68641 caatttatcg acctgcagcg cgttgatccg ccaaagaaac cgctgaagat ccgtaaaatt
    68701 gagtttgttg aaatttacga gccgttttcc gaaggccagg ccaaagcgca ggctgaccgc
    68761 tgtctgtcgt gcggcaaccc atactgcgag tggaaatgcc cggtacacaa ctacatccca
    68821 aactggctga agctcgccaa cgaggggcgt atttttgaag cggcagaact gtcgcatcag
    68881 accaacacac tgccggaagt atgcggacgt gtctgcccgc aagaccgtct gtgcgaaggt
    68941 tcctgcaccc tgaacgatga gttcggtgcg gtgaccatcg gcaacattga gcgttatatc
    69001 aatgataaag cgttcgaaat gggctggcgt ccggatatgt ccggcgtgaa gcaaaccggt
    69061 aaaaaagtgg cgattatcgg tgcaggcccg gcaggtctgg cgtgtgcgga tgtcctgacg
    69121 cgtaacggcg taaaagccgt tgtcttcgac cgtcatccag aaattggcgg gctgctgacc
    69181 ttcggtattc cggccttcaa gctggaaaaa gaggtaatga cgcgtcgccg tgaaatcttc
    69241 accggcatgg gtattgaatt caaactcaat accgaagtgg gccgcgacgt acagctggac
    69301 gatctgctga gtgattacga tgccgtgttc cttggcgttg ggacttatca gtcaatgcgc
    69361 ggcgggctgg aaaacgaaga cgccgatggc gtgtacgcag cgctgccgtt ccttatcgcc
    69421 aacaccaaac agttaatggg ctttggtgaa acccgcgacg aaccgttcgt cagcatggaa
    69481 ggcaaacgcg tggtagtact tggcggtggc gacactgcga tggactgcgt gcgcacgtct
    69541 gtacgccagg gggcaaagca cgttacctgt gcctatcgtc gtgatgaaga gaacatgccg
    69601 ggttcccgcc gcgaagtgaa aaacgcgcgg gaagaaggcg tagagttcaa attcaacgtc
    69661 cagccactgg ggattgaagt gaacggtaac ggcaaagtca gcggcgtaaa aatggtacgt
    69721 accgaaatgg gcgaaccgga tgccaaaggc cgtcgccgcg cggagatcgt tgcaggttcc
    69781 gaacatatcg ttccggcaga tgcggtgatc atggcgtttg gtttccgtcc acacaacatg
    69841 gaatggctgg caaaacacag cgtcgagctg gattcacaag gccgcatcat cgctccggaa
    69901 ggcaacgaca acgctttcca gaccagcaac ccgaaaatct ttgctggcgg cgatatcgtc
    69961 cgtggttccg atctggtcgt taccgccatc gccgaaggtc gcaaagcggc agacggcatt
    70021 atgaactggc tggaagttta agctctgtcc cagcgccgcc tcctcagaaa agagtcggcg
    70081 ctgaaattct cctttctttc tggaaatcgc ctcgcataat ctgaagaaaa ccaatgtaat
    70141 acgctcatct ggattgttac cggtagatgc ccggcgtata gctgattcca ttattcaacg
    70201 cagcgccata taaagggaca gataatggaa tcgctctcag agagaacctc aacaggctac
    70261 cagcaaatcc acaacggcat tattcatctg gtcgatagcg cccgaacgga aacggtacgt
    70321 agcgttaacg cgctgatgac cgcgacgtac tgggaaattg gccgacggat tgtcgaattt
    70381 gaacaaggtg gtgaggccag agcagcttat ggtgcacagt tgattgatcg gctatcgaag
    70441 gatttaagtc gacggtataa acgtggggtc tctgcaaaaa acttacgcca aatgaggctt
    70501 ttttacctct tttttcaaca tgttgaaatt cgccagacag tgtctggcga attaacacca
    70561 ttgggaattc cgcagacacc atctgcagaa ttcccatcgg caaagatttg gcagacgctg
    70621 tctgccaaat tgagaggctg tttgatattt ccacagcttt tgtattgcat ctcctatttt
    70681 cggtttgtat attcatgcaa ataaacgaag
//
LOCUS       DAJTRC010000007        67305 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.007, whole genome shotgun sequence.
ACCESSION   DAJTRC010000007 DAJTRC010000000
VERSION     DAJTRC010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.007"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            257..896
                     /locus_tag="OO357_000515"
                     /pseudo
     CDS             257..896
                     /locus_tag="OO357_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416774.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
     gene            999..1322
                     /locus_tag="OO357_000516"
     CDS             999..1322
                     /locus_tag="OO357_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1877 family protein"
                     /protein_id="HCR5885573.1"
                     /translation="MGMIGYFAEIDSEKINQLLESTEKPLMDNIHDTLSGLRRLESLD
                     RSELRKQFSIKRLNEMEIYPGVTFSEELEGQLFASIMLDMEKLISAYRRMLRQGNHAL
                     TVIVG"
     gene            complement(1389..2846)
                     /gene="nuoN"
                     /locus_tag="OO357_000517"
     CDS             complement(1389..2846)
                     /gene="nuoN"
                     /locus_tag="OO357_000517"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416779.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HCR5885574.1"
                     /translation="MTITPQNLIALLPLLIVGLTVVVVMLSIAWRRNHFLNATLSVIG
                     LNAALVSLWFVGQAGAMDVTPLMRVDGFAMLYTGLVLLASLATCTFAYPWLEGYNDNK
                     DEFYLLVLIAALGGILLANANHLASLFLGIELISLPLFGLVGYAFRQKRSLEASIKYT
                     ILSAAASSFLLFGMALVYAQSGDLSFVALGKNLGDGMLNEPLLLAGFGMMIVGLGFKL
                     SLVPFHLWTPDVYQGAPAPVSTFLATASKIAIFGVVMRLFLYAPVGDSEAIRVVLAII
                     AFASIIFGNLMALSQTNIKRLLGYSSISHLGYLLVALIALQTGEMSMEAVGVYLAGYL
                     FSSLGAFGVVSLMSSPYRGPDADSLFSYRGLFWHRPILAAVMTVMMLSLAGIPMTLGF
                     IGKFYVLAVGVQAHLWWLVGAVVVGSAIGLYYYLRVAVSLYLHAPEQPGRDAPSNWQY
                     SAGGIVVLISALLVLVLGVWPQPLISIVRLAMPLM"
     gene            complement(2853..4382)
                     /gene="nuoM"
                     /locus_tag="OO357_000518"
     CDS             complement(2853..4382)
                     /gene="nuoM"
                     /locus_tag="OO357_000518"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HCR5885575.1"
                     /translation="MLLPWLILIPFIGGFLCWQTERFGVKVPRWIALITMGLTLALSL
                     QLWLQGGYSLTQSAGIPQWQSEFDMPWIPRFGISIHLAIDGLSLLMVVLTGLLGVLAV
                     LCSWKEIEKYQGFFHLNLMWILGGVIGVFLAIDMFLFFFFWEMMLVPMYFLIALWGHK
                     ASDGKTRITAATKFFIYTQASGLVMLIAILALVFVHYNATGVWTFNYEELLNTPMSSG
                     VEYLLMLGFFIAFAVKMPVVPLHGWLPDAHSQAPTAGSVDLAGILLKTAAYGLLRFSL
                     PLFPNASAEFAPIAMWLGVIGIFYGAWMTFAQTDIKRLIAYTSVSHMGFVLIAIYTGS
                     QLAYQGAVIQMIAHGLSAAGLFILCGQLYERIHTRDMRMMGGLWSKMKWLPALSLFFA
                     VATLGMPGTGNFVGEFMILFGSFQVVPVITVISTFGLVFASVYSLAMLHRAYFGKAKS
                     QIASQELPGMSLRELFMILLLVVLLVLLGFYPQPILDTSHSAIGNIQQWFVNSVTTTR
                     P"
     gene            complement(4546..6387)
                     /gene="nuoL"
                     /locus_tag="OO357_000519"
     CDS             complement(4546..6387)
                     /gene="nuoL"
                     /locus_tag="OO357_000519"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HCR5885576.1"
                     /translation="MNMLALTIILPLIGFVLLAFSRGRWSENVSAIVGVGSVGLAALV
                     TAFIGVDFFANGEQAYSQPLWTWMSVGDFNIGFNLVLDGLSLTMLSVVTGVGFLIHMY
                     ASWYMRGEEGYSRFFAYTNLFIASMVVLVLADNLLLMYLGWEGVGLCSYLLIGFYYTD
                     PKNGAAAMKAFVVTRVGDVFLAFALFILYNELGTLNFRDMVELAPAHFADGNNMLMWA
                     TLMLLGGAVGKSAQLPLQTWLADAMAGPTPVSALIHAATMVTAGVYLIARTHGLFLMT
                     PEVLHLVGIVGAVTLLLAGFAALVQTDIKRVLAYSTMSQIGYMFLALGVQAWDAAIFH
                     LMTHAFFKALLFLASGSVILACHHEQNIFKMGGLRKSIPLVYLCFLVGGAALSALPLV
                     TAGFFSKDEILAGAMANGHINLMVAGLVGAFMTSLYTFRMIFIVFHGKEQIHAHAVKG
                     VTHSLPLIVLLILSTFVGALIVPPLQGVLPQTTELAHGSMLTLEITSGVVAVVGILLA
                     AWLWLGKRSLVTSIANSAPGRLLSTWWYNAWGFDWLYDKVFVKPFLGIAWLLKRDPLN
                     SMMNIPAVLSRFAGKGLLLSENGYLRWYVASMSIGAVVVLALLMVLR"
     gene            complement(6384..6686)
                     /gene="nuoK"
                     /locus_tag="OO357_000520"
     CDS             complement(6384..6686)
                     /gene="nuoK"
                     /locus_tag="OO357_000520"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HCR5885577.1"
                     /translation="MIPLQHGLILAAILFVLGLTGLVIRRNLLFMLIGLEIMINASAL
                     AFVVAGSYWGQTDGQVMYILAISLAAAEASIGLALLLQLHRRRQNLNIDSVSEMRG"
     gene            complement(6683..7237)
                     /gene="nuoJ"
                     /locus_tag="OO357_000521"
     CDS             complement(6683..7237)
                     /gene="nuoJ"
                     /locus_tag="OO357_000521"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HCR5885578.1"
                     /translation="MEFAFYICGLIAILATLRVITHTNPVHALLYLIISLLAISGVFF
                     SLGAYFAGALEIIVYAGAIMVLFVFVVMMLNLGGSEIEQERQWLKPQVWIGPAILSAI
                     MLVVIVYAILGVNDQGIDGTPISAKAVGITLFGPYVLAVELASMLLLAGLVVAFHVGR
                     EERAGEVLSNRKDDSAKRKTEEHA"
     gene            complement(7249..7791)
                     /gene="nuoI"
                     /locus_tag="OO357_000522"
     CDS             complement(7249..7791)
                     /gene="nuoI"
                     /locus_tag="OO357_000522"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015959823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HCR5885579.1"
                     /translation="MTLKELLVGFGTQVRSIWMIGLHAFAKRETRMYPEEPVYLPPRY
                     RGRIVLTRDPDGEERCVACNLCAVACPVGCISLQKAETKDGRWYPEFFRINFSRCIFC
                     GLCEEACPTTAIQLTPDFEMGEYKRQDLVYEKEDLLISGPGKYPEYNFYRMAGMAIDG
                     KDKGEAENEAKPIDVKSLLP"
     gene            complement(7806..8783)
                     /gene="nuoH"
                     /locus_tag="OO357_000523"
     CDS             complement(7806..8783)
                     /gene="nuoH"
                     /locus_tag="OO357_000523"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015875065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HCR5885580.1"
                     /translation="MSWISPELIEILLTILKAVVILLVVVTCGAFMSFGERRLLGLFQ
                     NRYGPNRVGWGGSLQLVADMIKMFFKEDWIPKFSDRVIFTLAPMIAFTSLLLAFAIVP
                     VSPGWVVADLNIGILFFLMMAGLAVYAVLFAGWSSNNKYSLLGAMRASAQTLSYEVFL
                     GLSLMGVVAQAGSFNMTDIVNSQAHVWNVIPQFFGFITFAIAGVAVCHRHPFDQPEAE
                     QELADGYHIEYSGMKFGLFFVGEYIGIVTISALMVTLFFGGWQGPLLPPFIWFALKTA
                     FFMMMFILIRASLPRPRYDRVMSFGWKICLPLTLINLLVTAAVILWQAQ"
     gene            complement(8780..11506)
                     /gene="nuoG"
                     /locus_tag="OO357_000524"
     CDS             complement(8780..11506)
                     /gene="nuoG"
                     /locus_tag="OO357_000524"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HCR5885581.1"
                     /translation="MATIHVDGKEYEVNGADNLLEACLSLGLDIPYFCWHPALGSVGA
                     CRQCAVKQYQNAEDTRGRLVMSCMTPASDGTFISIDDEEAKQFRESVVEWLMTNHPHD
                     CPVCEEGGNCHLQDMTVMTGHSFRRYRFTKRTHRNQDLGPFISHEMNRCIACYRCVRY
                     YKDYADGTDLGVYGAHDNVYFGRPEDGTLESEFSGNLVEICPTGVFTDKTHSERYNRK
                     WDMQFAPSICQQCSIGCNISPGERYGELRRIENRYNGTVNHYFLCDRGRFGYGYVNLK
                     DRPRQPVQRRGDDFITLNAEQAMQGAADILRQSKKVIGIGSPRASVESNFALRELVGE
                     ENFYTGIAHGEQERLQLALKVLREGGIYTPALREIESYDAVLVLGEDVTQTGARVALA
                     VRQAVKGKAREMAAAQKVADWQIAAILNIGQRAKHPLFVTNVDDTRLDDIAAWTYRAP
                     VEDQARLGFAIAHALDNSAPAVDGIEPELQNKIDVIVQALAGAKKPLIISGTNAGSIE
                     VIQAAANVAKALKGRGADVGITMIARSVNSMGLGIMGGGSLEEALTELETGRADAVVV
                     LENDLHRYASATRVNAALAKAPLVMVVDHQRTAIMENAHLVLSSASFAESDGTVINNE
                     GRAQRFFQVYDPAYYDSKTVMLESWRWLHSLHSTLLSREVDWTQLDHVIDAVVAKIPE
                     LAGIKDAAPDATFRIRGQKLAREPHRYSGRTAMRANISVHEPRQPQDIDTMFTFSMEG
                     NNQPTAHRSQVPFAWAPGWNSPQAWNKFQDEVGGKLRFGDPGVRLFETSENGLDYFTS
                     VPARFQPQDGKWRIAPYYHLFGSDELSQRAPVFQSRMPQPYIKLNPADAAKLGVNAGT
                     RVSFSYDGNTVTLPVEIAEGLTAGQVGLPMGMSGIAPVLAGAHLEDLKEAQQ"
     gene            complement(11559..12896)
                     /gene="nuoF"
                     /locus_tag="OO357_000525"
     CDS             complement(11559..12896)
                     /gene="nuoF"
                     /locus_tag="OO357_000525"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HCR5885582.1"
                     /translation="MKNIIRTPETHPLTWRLRDDKQPVWLDEYRSKNGYEGARKALTG
                     LSPDEIVNQVKDAGLKGRGGAGFSTGLKWSLMPKDESMNIRYLLCNADEMEPGTYKDR
                     LLMEQLPHLLVEGMLISAFALKAYRGYIFLRGEYIEAAVNLRRAIAEATEAGLLGKNI
                     MGTGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPATSGVWGKPTCVNN
                     VETLCNVPAILANGVEWYQNISKSKDAGTKLMGFSGRVKNPGLWELPFGTTAREILED
                     YAGGMRDGLKFKAWQPGGAGTDFLTEAHLDLPMEFESIGKAGSRLGTALAMAVDHEIN
                     MVSLVRNLEEFFARESCGWCTPCRDGLPWSVKILRALERGEGQPGDIETLEQLCRFLG
                     PGKTFCAHAPGAVEPLQSAIKYFREEFEAGIKQPFSNTHLINGIQPNLLKERW"
     gene            complement(12893..13393)
                     /gene="nuoE"
                     /locus_tag="OO357_000526"
     CDS             complement(12893..13393)
                     /gene="nuoE"
                     /locus_tag="OO357_000526"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE"
                     /protein_id="HCR5885583.1"
                     /translation="MHENQQPQTEAFELSAAEREAIEHEMHHYEDPRAASIEALKIVQ
                     KQRGWVPDGAIHAIADVLGIPASDVEGVATFYSQIFRQPVGRHVIRYCDSVVCHINGY
                     QGIQAALEKKLNIKPGQTTFDGRFTLLPTCCLGNCDKGPNMMIDEDTHAHLTPEAIPE
                     LLERYK"
     gene            complement(13396..15198)
                     /gene="nuoC"
                     /locus_tag="OO357_000527"
     CDS             complement(13396..15198)
                     /gene="nuoC"
                     /locus_tag="OO357_000527"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C/D"
                     /protein_id="HCR5885584.1"
                     /translation="MVNNMTDLTAQEPAWQTRDHLDDPVIGELRNRFGPDAFTVQATR
                     TGVPVVWIKREQLLEVGDFLKKLPKPYVMLFDLHGMDERLRTHREGLPAADFSVFYHL
                     ISIDRNRDIMLKVALAENDLHVPTFTKLFPNANWYERETWDLFGITFDGHPNLRRIMM
                     PQTWKGHPLRKDYPARATEFSPFELTKAKQDLEMEALTFKPEEWGMKRGTENEDFMFL
                     NLGPNHPSAHGAFRIVLQLDGEEIVDCVPDIGYHHRGAEKMGERQSWHSYIPYTDRIE
                     YLGGCVNEMPYVLAVEKLAGITVPDRVNVIRVMLSELFRINSHLLYISTFIQDVGAMT
                     PVFFAFTDRQKIYDLVEAITGFRMHPAWFRIGGVAHDLPRGWDRLLREFLDWMPKRLA
                     SYEKAALQNTILKGRSQGVAAYGAKEALEWGTTGAGLRATGIDFDVRKARPYSGYENF
                     DFEIPVGGGVSDCYTRVMLKVEELRQSLRILEQCLNNMPEGPFKADHPLTTPPPKERT
                     LQHIETLITHFLQVSWGPVMPANESFQMIEATKGINSYYLTSDGSTMSYRTRIRTPSY
                     AHLQQIPAAIRGSLVSDLIVYLGSIDFVMSDVDR"
     gene            complement(15292..15954)
                     /gene="nuoB"
                     /locus_tag="OO357_000528"
     CDS             complement(15292..15954)
                     /gene="nuoB"
                     /locus_tag="OO357_000528"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoB"
                     /protein_id="HCR5885585.1"
                     /translation="MDYTLTRIDPNGENDRYPLQKQEIVTDPLEQEVNKNVFMGKLND
                     MVNWGRKNSIWPYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADLMVVAGTC
                     FTKMAPVIQRLYDQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFIPVDVYIPGCPP
                     RPEAYMQALMLLQESIGKERRPLSWVVGDQGVYRANMQSERERKRGERIAVTNLRTPD
                     EI"
     gene            complement(15970..16413)
                     /gene="nuoA"
                     /locus_tag="OO357_000529"
     CDS             complement(15970..16413)
                     /gene="nuoA"
                     /locus_tag="OO357_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoA"
                     /protein_id="HCR5885586.1"
                     /translation="MSMSTSTEVIAHHWAFAIFLIVAIGLCCLMLVGGWFLGGRARAR
                     SKNVPFESGIDSVGSARLRLSAKFYLVAMFFVIFDVEALYLFAWSTSIRESGWVGFVE
                     AAIFIFVLLAGLVYLVRIGALDWTPARSRRERMNPETNSIANRQR"
     gene            complement(17044..17982)
                     /gene="lrhA"
                     /locus_tag="OO357_000530"
     CDS             complement(17044..17982)
                     /gene="lrhA"
                     /locus_tag="OO357_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LrhA"
                     /protein_id="HCR5885587.1"
                     /translation="MISANRPIINLDLDLLRTFVAVADLNTFAAAAAAVCRTQSAVSQ
                     QMQRLEQLVGKELFARHGRNKLLTEHGIQLLGYARKILRFNDEACSSLMFSNLQGVLT
                     IGASDESADTILPFLLNRVSSVYPKLALDVRVKRNAYMAEMLESQEVDLMVTTHRPSA
                     FKALNLRTSPTHWYCAAEYVLQKGEPIPLVLLDDPSPFRDMVLATLNKVDIPWRLAYV
                     ASTLPAVRAAVKAGLGVTARPVEMMSPDLRVLSGVDGLPPLPDTEYLLCYDPSSNNEL
                     AQVIYQAMESYHNPWQYSPMSAPEGDDSLLIERDIE"
     gene            18901..20118
                     /gene="alaA"
                     /locus_tag="OO357_000531"
     CDS             18901..20118
                     /gene="alaA"
                     /locus_tag="OO357_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine transaminase AlaA"
                     /protein_id="HCR5885588.1"
                     /translation="MSPIEKSSKLENVCYDIRGPVLKEAKRLEEEGNKVLKLNIGNPA
                     PFGFDAPDEILVDVIRNLPTAQGYCDSKGLYSARKAIMQHYQARGMRDVTVEDIYIGN
                     GVSELIVQAMQALLNSGDEMLVPAPDYPLWTAAVSLSSGKAVHYLCDESSDWFPDLDD
                     IRAKITPRTRGIVIINPNNPTGAVYSKELLMEIVEIARQHNLIIFADEIYDKILYDDA
                     EHHSIAPLAPDLLTITFNGLSKTYRVAGFRQGWMVLNGPKKHAKGYIEGLEMLASMRL
                     CANVPAQHAIQTALGGYQSISEFITPGGRLYEQRNRAWELINDIPGVSCVKPRGALYM
                     FPKIDAKRFNIHDDQKMVLDFLLQEKVLLVQGTAFNWPWPDHFRIVTLPRVDDIELSL
                     SKFARFLSGYHQL"
     gene            20202..20801
                     /gene="yfbR"
                     /locus_tag="OO357_000532"
     CDS             20202..20801
                     /gene="yfbR"
                     /locus_tag="OO357_000532"
                     /EC_number="3.1.3.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-deoxynucleotidase"
                     /protein_id="HCR5885589.1"
                     /translation="MKQSHFFAHLSRLKLINRWPLMRNVRTENVSEHSLQVAMVAHAL
                     AAIKNRKFGGNVNAERIALLAMYHDASEVLTGDLPTPVKYFNAQIAQEYKAIEKIAQQ
                     KLVDMVPEELRDIFAPLIDEHAYSDEEKSLVKQADALCAYLKCLEELAAGNNEFLLAK
                     TRLEATLEARRSQEMDYFMEVFVPSFHLSLDEISQDSPL"
     gene            complement(20860..22692)
                     /locus_tag="OO357_000533"
     CDS             complement(20860..22692)
                     /locus_tag="OO357_000533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HCR5885590.1"
                     /translation="MNGELIWVLSLLAVAIVLFATGRVRMDAVALFVIVAFALSGTLT
                     VPEVFSGFSDPNVVLIAALFIIGDGLVRTGVATVMGTWLVKVAGNSEIKMLVLLMLTV
                     AGLGAFMSSTSVVAIFIPVVLSVAMRMQTSPSRLMMPLSFAGLISGMMTLVATPPNLV
                     VNSELLREGYHGFSFFSVTPIGLVVLVLGILYMLVMRFMLKGDTQTPQREGWTRRTFR
                     DLIREYRLTGRARRLAIRPGSPMIGQRLDDLKLRERYGANVIGVERWRRFRRVIVNVN
                     GVSEFRARDVLLIDMSAADVDLRQFCSEQLLEPMVLRGEYFSDQALDVGMAEISLIPE
                     SELIGKSVREIGFRTRYGLNVVGLKRNGVALEGSLADEPLLLGDIILVVGNWKLIGML
                     AKQGRDFVALNLPEEVSEASPAHSQAPHAIFCLVLMVALMLTNEIPNPVAAIIACLLM
                     GKFRCIDAESAYKSIHWPSIILIVGMMPFAVALQKTGGVALAVKGLMDIGGGYGPHMM
                     LGCLFVLSAVIGLFISNTATAVLMAPIALAAAKTMGVSPYPFAMVVAMAASAAFMTPV
                     SSPVNTLVLGPGNYSFSDFVKLGVPFTIIVMAVCVVMIPMLFPF"
     gene            complement(22779..23429)
                     /gene="hxpA"
                     /locus_tag="OO357_000534"
     CDS             complement(22779..23429)
                     /gene="hxpA"
                     /locus_tag="OO357_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpA"
                     /protein_id="HCR5885591.1"
                     /translation="MRCKGFLFDLDGTLVDSLPAVERAWSNWARRHGLAPEEVLAFIH
                     GKQAITSLRHFMAGKSEADIAAEFTRLEQIEATETEGITALPGAIALLNHLNKAGIPW
                     AIVTSGSMPVARARHKIAGLPAPEVFVTAERVKRGKPEPDAYLLGAQLLGLAPQECVV
                     VEDAPAGVLSGLAAGCHVIAVNAPADTPRLNEVDLVLHSLEQITVTKQPNGDVIIQ"
     gene            complement(23440..23934)
                     /locus_tag="OO357_000535"
     CDS             complement(23440..23934)
                     /locus_tag="OO357_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007371080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HCR5885592.1"
                     /translation="MEMTNAQRLILSNQYKMMTMLDPANAERYRRLQTIIERGYGLQM
                     RELDREFGELKEETCRTIIDIMDMYHALYVSWSNLQDQQSIDERRVTFLGFDAATEAR
                     YLGYVRFMVNVEGRYTHFDAGTHGFNAQTPMWEKYQRMLNVWHACPRQYHLSANEINQ
                     IINA"
     gene            complement(24017..24472)
                     /locus_tag="OO357_000536"
     CDS             complement(24017..24472)
                     /locus_tag="OO357_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF412 domain-containing protein"
                     /protein_id="HCR5885593.1"
                     /translation="MSTPDNRSVNFFSLFRRGQHYSKTWPLEKRLAPVFVENRVIKMT
                     RYAIRFMPPIAVFTLCWQIALGGLLGPAVATALFALSLPMQGLWWLGKRSVTPLPPAI
                     LNWFYEVRGKLQESGQVLAPVEGKPDYQALADTLKRAFKQLDKTFLDDL"
     gene            24810..26012
                     /gene="ackA"
                     /locus_tag="OO357_000537"
     CDS             24810..26012
                     /gene="ackA"
                     /locus_tag="OO357_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HCR5885594.1"
                     /translation="MSSKLVLVLNCGSSSLKFAIIDAVNGEEYLSGLAECFHLPEARI
                     KWKMDGNKQEAALGAGAAHSEALNFIVNTILAQKPELSAQLTAIGHRIVHGGEKYTSS
                     VVIDESVIQGIKDAASFAPLHNPAHLIGIEEALKSFPQLKDKNVAVFDTAFHQTMPEE
                     SYLYALPYNLYKEHGIRRYGAHGTSHFYVTQEAAKMLNKPVEELNIITCHLGNGGSVS
                     AIRNGKCVDTSMGLTPLEGLVMGTRSGDIDPAIIFHLHDTLGMSVDAINKLLTKESGL
                     LGLTEVTSDCRYVEDNYATKEDAKRAMDVYCHRLAKYIGAYTALMDGRLDAVAFTGGI
                     GENAAMVRELSLGKLGVLGFEVDHERNLAARFGKSGFINKEGTRPAVVIPTNEELVIA
                     QDASRLTA"
     gene            26087..28231
                     /gene="pta"
                     /locus_tag="OO357_000538"
     CDS             26087..28231
                     /gene="pta"
                     /locus_tag="OO357_000538"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HCR5885595.1"
                     /translation="MSRIIMLIPTGTSVGLTSVSLGVIRAMERKGVRLSVFKPIAQPR
                     TGGDAPDQTTTIVRANSSTTTAAEPLKMSYVEGLLSSNQKDVLMEEIVANYHANTKDA
                     EVVLVEGLVPTRKHQFAQSLNYEIAKTLNAEIVFVMSQGTDTPEQLKERIELTRNSFG
                     GAKNTNITGVIVNKLNAPVDEQGRTRPDLSEIFDDSSKAKVNNVDPAKLQESSPLPVL
                     GAVPWSFDLIATRAIDMARHLNATIINEGDINTRRVKSVTFCARSIPHMLEHFRAGSL
                     LVTSADRPDVLVAACLAAMNGVEIGALLLTGGYEMDARISKLCERAFATGLPVFMVNT
                     NTWQTSLSLQSFNLEVPVDDHERIEKVQEYVANYINADWIESLTATSERSRRLSPPEF
                     RYQLTELARKAGKRIVLPEGDEPRTVKAAAICAERGIATCVLLGNPAEINRVAASQGV
                     ELGAGIEIVDPEVVRESYVGRLVELRKNKGMTETVAREQLEDNVVLGTLMLEQDEVDG
                     LVSGAVHTTANTIRPPLQLIKTAPGSSLVSSVFFMLLPEQVYVYGDCAINPDPTAEQL
                     AEIAIQSADSAAAFGIEPRVAMLSYSTGTSGAGSDVEKVREATRLAQEKRPDLMIDGP
                     LQYDAAVMADVAKSKAPNSPVAGRATVFIFPDLNTGNTTYKAVQRSADLISIGPMLQG
                     MRKPVNDLSRGALVDDIVYTIALTAIQSAQQQ"
     gene            28421..29941
                     /gene="yfcC"
                     /locus_tag="OO357_000539"
     CDS             28421..29941
                     /gene="yfcC"
                     /locus_tag="OO357_000539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HCR5885596.1"
                     /translation="MSAITESKPTRRWAMPDTLVIIFFVAILTSLATWVVPVGMFDSQ
                     EVQYQVDGQTKTRKVVDPHSFRILTNEAGEPEYHRVQLFTTGDERPGLMNFPFEGLTS
                     GSKYGTAVGIIMFMLVIGGAFGIVMRTGTIDNGILALIRHTRGNEILFIPALFILFSL
                     GGAVFGMGEEAVAFAIIIAPLMVRLGYDSITTVLVTYIATQIGFASSWMNPFCVVVAQ
                     GIAGVPVLSGSGLRIVVWVIATLIGLIFTMVYASRVKKNPLLSRVHESDRFFREKQAD
                     VEQRPFTFGDWLVLIVLTAVMVWVIWGVIVNAWFIPEIASQFFTMGLVIGIIGVVFRL
                     NGMTVNTMASSFTEGARMMIAPALLVGFAKGILLLVGNGEAGNASVLNTILNSIANAI
                     SGLDNAVAAWFMLLFQAVFNFFVTSGSGQAALTMPLLAPLGDLVGVNRQVTVLAFQFG
                     DGFSHIIYPTSASLMATLGVCRVDFRNWLKVGATLLGLLFIMSSVVVIGAQLMGYH"
     gene            complement(29974..30516)
                     /gene="yfcD"
                     /locus_tag="OO357_000540"
     CDS             complement(29974..30516)
                     /gene="yfcD"
                     /locus_tag="OO357_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase YfcD"
                     /protein_id="HCR5885597.1"
                     /translation="MEQRRLASTEWVDIVNEENEVIAQASREQMRAQCLRHRATYIVV
                     HDGMGKILVQRRTETKDFLPGMLDATAGGVVQADEQLLESARREAEEELGIAGVPFAE
                     HGQFYFEDKNCRVWGALFSCVSHGPFALQEDEVSGVCWLTPEEITARCDEFTPDSLKA
                     LALWMKRNAKNEAVETETAE"
     gene            complement(30574..31125)
                     /gene="yfcE"
                     /locus_tag="OO357_000541"
     CDS             complement(30574..31125)
                     /gene="yfcE"
                     /locus_tag="OO357_000541"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708182.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiesterase"
                     /protein_id="HCR5885598.1"
                     /translation="MKLMFASDIHGSLPATERVVELFVQSGAQWLVILGDVLNHGPRN
                     ALPEGYAPAKVAERLNEVAHKVIAVRGNCDSEVDQMMLHFPITAPWQQVLLEKQRLFL
                     THGHLFGPENLPALNQNDVLVYGHTHLPVAEQRGEIFHFNPGSVSIPKGGNPAGYGML
                     DNDVLSVIALNDQSIIAQVAINP"
     gene            complement(31181..31825)
                     /gene="yfcF"
                     /locus_tag="OO357_000542"
     CDS             complement(31181..31825)
                     /gene="yfcF"
                     /locus_tag="OO357_000542"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase"
                     /protein_id="HCR5885599.1"
                     /translation="MSKPAITLWSDAHFFSPYVLSAWVALQEKGLSFHIKTIDLDSGE
                     HLQPTWQGYGQTRRVPLLQIDDFELSESSAIAEYLEDRFAPPTWERIYPLDLENRARA
                     RQIQAWLRSDLMPIREERPTDVVFAGAKKAPLTAEGKASAEKLFAMAEHLLALGQPNL
                     FGEWCIADTDLALMINRLVLHGDEVPERLVDYATFQWQRASVQRFIALSAKQSG"
     gene            31961..32608
                     /gene="yfcG"
                     /locus_tag="OO357_000543"
     CDS             31961..32608
                     /gene="yfcG"
                     /locus_tag="OO357_000543"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GSH-dependent disulfide bond oxidoreductase"
                     /protein_id="HCR5885600.1"
                     /translation="MIDLYFAPTPNGHKITLFLEEAELDYRLIKVDLGKGGQFRPEFL
                     RISPNNKIPAIVDHSPADGGEPLSLFEAAAILLYLAEKTGLFLSHETRERAATLQWLF
                     WQVGGLGPMLGQNHHFNHAAPQTIPYAIERYQVETQRLYHVLNKRLENSPWLGGENYS
                     IADIACWPWVNAWTRLRIDLAMYPAVKNWHERIRSRPATGQALLKAQLGDERSDS"
     gene            32665..33027
                     /gene="folX"
                     /locus_tag="OO357_000544"
     CDS             32665..33027
                     /gene="folX"
                     /locus_tag="OO357_000544"
                     /EC_number="5.1.99.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate 2'-epimerase"
                     /protein_id="HCR5885601.1"
                     /translation="MAQPAAIIRIKNLRLRTFIGIKEEEINNRQDIVINVTIHYPADK
                     ARTSEDINDALNYRTVTKNIIQHVENNRFSLLEKLTQDVLDIAREHHWVTYAEVEIDK
                     LHALRYADSVSMTLSWQR"
     gene            33048..33941
                     /locus_tag="OO357_000545"
     CDS             33048..33941
                     /locus_tag="OO357_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01777 family oxidoreductase"
                     /protein_id="HCR5885602.1"
                     /translation="MNIVITGGTGLIGRHLIPRLLELGHQITVVTRNPQKASSVLGPR
                     VTLWQGLADQSNLNGVDAVINLAGEPIADKRWTHKQKERLCQSRWNITQKLVDLINAS
                     DTPPSVLISGSATGYYGDLGEVVVTEEEPPHNEFTHKLCARWEEIACRAQSDKTRVCL
                     LRTGVVLAPDGGILGKMLPPFRLGLGGPIGSGRQYLAWIHIDDMVNGILWLLDNELRG
                     PFNMVSPYPVRNEQFAHALGHALHRPAILRVPTTAIRLLMGESSVLVLGGQRALPKRL
                     EEAGFAFRWYDLEEALADVVR"
     gene            complement(33989..34879)
                     /gene="rpnB"
                     /locus_tag="OO357_000546"
     CDS             complement(33989..34879)
                     /gene="rpnB"
                     /locus_tag="OO357_000546"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnB"
                     /protein_id="HCR5885603.1"
                     /translation="MTISTTSTPHDAVFKSFLRHPDTARNFINIHLPAPLRKLCDLTT
                     LKLEPNSFIDEDLRQYYSDLLWSVKTQEGVGYIYVVIEHQSKPEELMAFRMMRYSIAA
                     MQNHLDAGYKELPLVIPMLFYHGCRSPYPYSLCWLDEFAEPAIARKIYSSAFPLVDIT
                     VVPDDEIMQHRKMALLELIQKHIRQRDLLGLVDQIVSLLVTGNTNDRQLKALFNYVLQ
                     TGDAQRFRAFIGEITERAPQEKEKLMTIADRLREEGAMQGKHEEALRIAQEMLDRGLD
                     RELVMMVTRLSPDDLIAQSH"
     gene            complement(35076..35849)
                     /gene="hisP"
                     /locus_tag="OO357_000547"
     CDS             complement(35076..35849)
                     /gene="hisP"
                     /locus_tag="OO357_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter ATP-binding protein
                     HisP"
                     /protein_id="HCR5885604.1"
                     /translation="MSENKLNVIDLHKRYGEHEVLKGVSLQANAGDVISIIGSSGSGK
                     STFLRCINFLEKPSEGSIVVNGQTINLVRDKDGQLKVADKNQLRLLRTRLTMVFQHFN
                     LWSHMTVLENVMEAPIQVLGLSKQEARERAVKYLAKVGIDERAQGKYPVHLSGGQQQR
                     VSIARALAMEPEVLLFDEPTSALDPELVGEVLRIMQQLAEEGKTMVVVTHEMGFARHV
                     STHVIFLHQGKIEEEGAPEQLFGNPQSPRLQQFLKGSLK"
     gene            complement(35857..36573)
                     /locus_tag="OO357_000548"
     CDS             complement(35857..36573)
                     /locus_tag="OO357_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5885605.1"
                     /translation="MIEILHEYWKPLLWTDGYRFTGVAITLWLLILSVVIGGVLALFL
                     AIGRVSSNKYIQFPIWLFTYIFRGTPLYVQLLVFYSGMYTLEIVKGTEFLNAFFRSGL
                     NCTVLALTLNTCAYTTEIFAGAIRSVPHGEIEAARAYGFSTFKMYRCIILPSALRIAL
                     PAYSNEVILMLHSTALAFTATVPDLLKIARDINAATYQPFTAFGIAAVLYLIISYVLI
                     SLFRRAEKRWLQHVKPSSTH"
     gene            complement(36570..37256)
                     /gene="hisQ"
                     /locus_tag="OO357_000549"
     CDS             complement(36570..37256)
                     /gene="hisQ"
                     /locus_tag="OO357_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter permease HisQ"
                     /protein_id="HCR5885606.1"
                     /translation="MLYGFSGVILQGALVTLELAISSVVLAVIIGLIGAGGKLSQNRL
                     SGLIFEGYTTLIRGVPDLVLMLLIFYGLQIALNMVTEAMGVGQIDIDPMVAGIITLGF
                     IYGAYFTETFRGAFMAVPKGHIEAATAFGFTRGQVFRRIMFPAMMRYALPGIGNNWQV
                     ILKSTALVSLLGLEDVVKATQLAGKSTWEPFYFAIVCGVIYLVFTTVSNGVLLFLERR
                     YSVGVKRADL"
     gene            complement(37346..38128)
                     /gene="hisJ"
                     /locus_tag="OO357_000550"
     CDS             complement(37346..38128)
                     /gene="hisJ"
                     /locus_tag="OO357_000550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ABC transporter substrate-binding
                     protein HisJ"
                     /protein_id="HCR5885607.1"
                     /translation="MKKLVLSLSLVLAFSSATAALAAIPQNIRIGTDPTYAPFESKNS
                     QGELVGFDIELAKELCKRINTQCTFVENPLDALIPSLKAKKIDAIMSSLSITEKRQQE
                     IAFTDKLYAADSRLVVAKNSDIQPTVESLKGKRVGVLQGTTQETFGNEHWAPKGIEIV
                     SYQGQDNIYSDLTAGRIDAAFQDEVAASEGFLKQPVGKDYKFGGPSVKDEKLFGVGTG
                     MGLRKEDNELREALNKAFAEMRADGTYEKLAKKYFDFDVYGG"
     gene            complement(38349..39131)
                     /gene="argT"
                     /locus_tag="OO357_000551"
     CDS             complement(38349..39131)
                     /gene="argT"
                     /locus_tag="OO357_000551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine/arginine/ornithine ABC transporter
                     substrate-binding protein ArgT"
                     /protein_id="HCR5885608.1"
                     /translation="MKKSILALSLLVGLSAAASSYAALPETVRIGTDTTYALFSSKDA
                     KGDFVGFDIDLGNEMCKRMQVKCTWVASDFDALIPSLKAKKIDAIISSLSITDKRQQE
                     IAFSDKLYAADSRLIAAKGSPIQPTLDSLKGKHVGVLQGSTQEAYANETWRSKGVDVV
                     AYANQDLVYSDLAAGRLDAALQDEVAASEGFLKQPAGKDFAFAGSSVKDKKYFGDGTG
                     VGLRKDYAELTAAFNKALGELRQDGTYDKMAKKYFDFNVYGD"
     gene            complement(39397..39966)
                     /gene="ubiX"
                     /locus_tag="OO357_000552"
     CDS             complement(39397..39966)
                     /gene="ubiX"
                     /locus_tag="OO357_000552"
                     /EC_number="2.5.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin prenyltransferase UbiX"
                     /protein_id="HCR5885609.1"
                     /translation="MKRLIVGISGASGAIYGVRLLQVLRDVTDIETHLVMSQAARQTL
                     SLETDFSLREVQALADVTLDARDISASISSGSFQTLGMVILPCSIKTLSGIVHSYTDG
                     LLTRAADVVLKERRPLVLCVRETPLHLGHLRLMTQAAEIGAVIMPPVPAFYHRPQSLD
                     DVINQTVNRVLDQFAITLPEDLFARWQGA"
     gene            complement(40061..41578)
                     /gene="purF"
                     /locus_tag="OO357_000553"
     CDS             complement(40061..41578)
                     /gene="purF"
                     /locus_tag="OO357_000553"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HCR5885610.1"
                     /translation="MCGIVGIAGVMPVNQSIYDALTVLQHRGQDAAGIITIDANNCFR
                     LRKANGLVSDVFEARHMQRLQGNMGIGHVRYPTAGSSSASEAQPFYVNSPYGITLAHN
                     GNLTNAHELRKKLFEEKRRHINTTSDSEILLNIFASELDNFRHYPLEADNIFAAIAAT
                     NRLIRGAYACVAMIIGHGMVAFRDPNGIRPLVLGKRDIDENRTEYMVASESVALDTLG
                     FDFLRDVAPGEAIYITEEGQLFTRQCADNPVSNPCLFEYVYFARPDSFIDKISVYSAR
                     VNMGTKLGEKIAREWEDLDIDVVIPIPETSCDIALEIARILGKPYRQGFVKNRYVGRT
                     FIMPGQQLRRKSVRRKLNANRAEFRDKNVLLVDDSIVRGTTSEQIIEMAREAGAKKVY
                     LASAAPEIRFPNVYGIDMPSATELIAHGREVDEIRQIIGADGLIFQDLNDLIDAVRAE
                     NPDIQQFECSVFNGVYVTKDVDQGYLDFLDTLRNDDAKAVQRQNEVENLEMHNEG"
     gene            complement(41615..42103)
                     /gene="cvpA"
                     /locus_tag="OO357_000554"
     CDS             complement(41615..42103)
                     /gene="cvpA"
                     /locus_tag="OO357_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin V production protein"
                     /protein_id="HCR5885611.1"
                     /translation="MVWIDYAIIAVIAFSSLVSLIHGFVREALSLVTWGCAFFVASHY
                     YTYLSVWFTGFEDELVRNGIAIAVLFIATLIVGAIVNFVIGQLVEKTGLSGTDRVLGV
                     CFGALRGVLIVAAILFFLDSFTGVSKSEDWSKSQLIPQFSFIIRWFFDYLQSSSSFLP
                     RA"
     gene            complement(42362..43024)
                     /gene="dedD"
                     /locus_tag="OO357_000555"
     CDS             complement(42362..43024)
                     /gene="dedD"
                     /locus_tag="OO357_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein DedD"
                     /protein_id="HCR5885612.1"
                     /translation="MASKFQNRLVGTIVLVALGVIVLPGLLDGQKKHYQDEFAAIPLV
                     PKAGDRDEPDMMPAATQALPTQPPEGAAEEVRAGDAAAPSLDPATIAANNTEFEPEPA
                     PVAPPKPKPVEPPKPKVEVPPAPKPEPKPVVEEKAAPTGKAYVVQLGALKNADKVNEI
                     VGKLRGAGYRVYTSPSTPVQGKITRILVGPDASKDKLKGSLGELKQLSGLSGVVMGYT
                     PN"
     gene            complement(43014..44282)
                     /gene="folC"
                     /locus_tag="OO357_000556"
     CDS             complement(43014..44282)
                     /gene="folC"
                     /locus_tag="OO357_000556"
                     /EC_number="6.3.2.12"
                     /EC_number="6.3.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tetrahydrofolate
                     synthase/dihydrofolate synthase"
                     /protein_id="HCR5885613.1"
                     /translation="MIIKRTPQAASPLASWLSYLENLHSKTIDLGLERVSQVAARLGV
                     LKPAPFVFTVAGTNGKGTTCRTLESILMAAGYKVGVYSSPHLVRYTERVRVQGQELPE
                     SAHTASFAEIESARGDISLTYFEYGTLSALWLFKQAQLDVVILEVGLGGRLDATNIVD
                     ADVAVVTSIALDHTDWLGPDRESIGLEKAGIFRSEKPAIVGEPEMPSTIADVAQEKGA
                     LLQRRGVEWNYSVTDHDWAFSDAHGTLENLPLPLVPQPNAATALAALRASGLEVSENA
                     IRDGIASAILPGRFQIVSESPRVIFDVAHNPHAAEYLTGRMKALPKNGRVLAVIGMLH
                     DKDIAGTLAWLKSVVDDWYCAPLEGPRGATAEQLLEHLGNGKSFDSVAQAWDAAMADA
                     KAEDTVLVCGSFHTVAHVMEVIDARRSGGK"
     gene            complement(44352..45266)
                     /gene="accD"
                     /locus_tag="OO357_000557"
     CDS             complement(44352..45266)
                     /gene="accD"
                     /locus_tag="OO357_000557"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HCR5885614.1"
                     /translation="MSWIERIKSNITPTRKASIPEGVWTKCDSCGQVLYRAELERNLE
                     VCPKCDHHMRMTARNRLHSLLDEGSLVELGSELEPKDELKFRDSKKYKDRLASAQKET
                     GEKDALVVMKGTLYGMPVVAAAFEFAFMGGSMGSVVGARFVRAVEQAQEDNCPLICFS
                     ASGGARMQEALMSLMQMAKTSAALAKMQERGLPYISVLTDPTMGGVSASFAMLGDLNI
                     AEPKALIGFAGPRVIEQTVREKLPPRFQRSEFLIEKGAIDMIVRRPEMRLKLASILAK
                     LMNLPAPNPEAPREGVVVPPVPDQEPEA"
     gene            complement(45422..46081)
                     /locus_tag="OO357_000558"
     CDS             complement(45422..46081)
                     /locus_tag="OO357_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5885615.1"
                     /translation="MDLIYFLIDFILHIDVHLAELVAEYGVWVYAILFLILFCETGLV
                     VMPFLPGDSLLFVAGALASLETNDLNVHMMVVLMLIAAIVGDAVNYTIGRLFGEKLFS
                     NPNSKIFRRSYLDKTHQFYEKHGGKTIILARFVPIVRTFAPFVAGMGHMSYRHFAAYN
                     VIGALLWVLLFTYAGYFFGTIPMVQDNLKLLIVGIIVVSILPGVIEIIRHKRAAARAA
                     K"
     gene            complement(46164..46976)
                     /gene="truA"
                     /locus_tag="OO357_000559"
     CDS             complement(46164..46976)
                     /gene="truA"
                     /locus_tag="OO357_000559"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HCR5885616.1"
                     /translation="MSDQQQLPVYKIALGIEYDGSKYYGWQRQNEVRSVQEKLEKALS
                     QVANEPITVFCAGRTDAGVHGTGQVVHFETTAQRKDAAWTLGVNANLPGDIAVRWVKA
                     VPDDFHARFSATARRYRYIIYNHRLRPAVLSKGVTHFYEPLDAERMHRAAQCLLGEND
                     FTSFRAVQCQSRTPWRNVMHINVTRHGPYVVVDIKANAFVHHMVRNIVGSLMEVGAHN
                     QPESWIAELLAAKDRTLAAATAKAEGLYLVAVDYPDRYDLPKPPMGPLFLAD"
     gene            complement(46976..47989)
                     /locus_tag="OO357_000560"
     CDS             complement(46976..47989)
                     /locus_tag="OO357_000560"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5885617.1"
                     /translation="MSEGWNIAVLGATGAMGEALLETLAERQFPVGEIYALARNESAG
                     EQLRFGGKTITVQDAAEFDWTQAQLAFFVAGKEATAAWVEEATNSGCLVIDSSGLFAL
                     EPDVPLVVPEVNPFVLTDYRNRNVIAVPDSLTSQLLAALKPLIDQGGLSRISVTSLIS
                     ASAQGKKAVDALAGQSAKLLNGIPIDEEDFFGRQLAFNMLPLLPDSEGSVREERRIVD
                     EVRKILQDEGLMISASVVQAPVFYGHAQMVNFEALRPLAAEEARDAFAQGEDIVLSEE
                     NEFPTQVGDASGTPHLSVGCVRNDYGMPEQVQFWSVADNVRFGGALMAVKIAEKLVQE
                     YLY"
     gene            complement(48055..49191)
                     /gene="pdxB"
                     /locus_tag="OO357_000561"
     CDS             complement(48055..49191)
                     /gene="pdxB"
                     /locus_tag="OO357_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-phosphoerythronate dehydrogenase PdxB"
                     /protein_id="HCR5885618.1"
                     /translation="MKILVDENMPYARDLFSRLSEVIAVPGRPIPVAQLADADALMVR
                     SVTKVNESLLAGKPIKFVGTATAGTDHVDEAWLKQAGIGFSAAPGCNAIAVVEYVFSS
                     LLMLAERDGFSLHDRTVGIVGVGNVGRRLQARLEALGIKTLLCDPPRADRGDEGDFRS
                     LDELVQRADILTFHTPLFKDGPYKTLHLADEKLIRSLKPGAILINACRGAVVDNTALL
                     TCLNEGQKLSVVLDVWEGEPELNVELLTKVDIGTPHIAGYTLEGKARGTTQVFEAYSK
                     FIGHEQHVALDTLLPAPEFGRITLHGLLDQPTLKRLVHLVYDVRRDDAPLRKVAGIPG
                     EFDKLRKNYLERREWSSLYVICDDASAASLLCKLGFNAVHHPAR"
     gene            49290..50285
                     /gene="flk"
                     /locus_tag="OO357_000562"
     CDS             49290..50285
                     /gene="flk"
                     /locus_tag="OO357_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulator Flk"
                     /protein_id="HCR5885619.1"
                     /translation="MIQPISGPPPGQPPGQGDNLPSGAGNQPLSSQQRTSLESLMTKV
                     TSLTQQQRAELWAGIRHDIGLSGDSPLLSRHFPAAEHNLAQRLLAAQKSHSARQLLAQ
                     LGEYLRLGNHRQAVTDYIRHNFGQTPLNQLSPEQLKTILTLLQEGKMVIPQPQQREAT
                     DRPLLPAEHNALKQLVTKLTAATGEPSKQIWQSMLELSGVKDGELIPAKLFNHLVTWL
                     QARQTLSQQNTPTLESLQMALKQPLDASELAALSAYIQQKYGLSAQSSLSSAQAEDIL
                     NQLYQRRVKGIDPRDMQPLLNPFPPMMDTLQNMATRPALWILLVAIILMLVWLVR"
     gene            complement(50282..51460)
                     /locus_tag="OO357_000563"
     CDS             complement(50282..51460)
                     /locus_tag="OO357_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5885620.1"
                     /translation="MTAVSQTETRSSASFSLFRISFAVFLTYMTVGLPLPVIPLFVHH
                     DLGYGNTMVGIAVGIQFLATVLTRGYAGRLADQYGAKRSALQGMLACGLAGGALLLAA
                     ILPVSAPFKFALLVIGRLILGFGESQLLTGALTWGLGIVGPKHSGKVMSWNGMAIYGA
                     LAVGAPLGLLIHSHYGFAALAITTMVLPLLAWACNGTVRKVPALAGERPSLWSVVGLI
                     WKPGLGLALQGVGFAVIGTFVSLYFASKGWAMAGFTLTAFGGAFVVMRVMFGWMPDRF
                     GGVKVAIVSLLVETVGLLLLWQAPGAWVALAGAALTGAGCSLIFPALGVEVVKRVPSQ
                     VRGTALGGYAAFQDIALGVSGPLAGMLATTFGYSSVFLAGAISAVLGIIVTILSFRRG
                     "
     gene            complement(51715..52956)
                     /gene="fabB"
                     /locus_tag="OO357_000564"
     CDS             complement(51715..52956)
                     /gene="fabB"
                     /locus_tag="OO357_000564"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HCR5885621.1"
                     /translation="MKRAVITGLGIVSSIGNNQQEVLASLREGRSGITFSQELKDSGM
                     RSHVWGNIKLDTTGLIDRKVVRFMSDASIYAFLSMEQAIADAGLSPEAYQNNPRVGLI
                     AGSGGGSPRFQVFGADAMRGPRGLKAVGPYVVTKAMASGVSACLATPFKIHGVNYSIS
                     SACATSAHCIGNAVEQIQLGKQDIVFAGGGEELCWEMACEFDAMGALSTKYNDTPEKA
                     SRTYDAHRDGFVIAGGGGMVVVEELEHALARGAYIYAEIVGYGATSDGADMVAPSGEG
                     AVRCMQMAMHGVDTPIDYLNSHGTSTPVGDVKELAAIREVFGDKSPAISATKAMTGHS
                     LGAAGVQEAIYSLLMLEHGFIAPSINIEELDEQAAGLNIVTETTDRELTTVMSNSFGF
                     GGTNATLVMRKLKRLIRSRPE"
     gene            53115..55121
                     /gene="mnmC"
                     /locus_tag="OO357_000565"
     CDS             53115..55121
                     /gene="mnmC"
                     /locus_tag="OO357_000565"
                     /EC_number="1.5.-.-"
                     /EC_number="2.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase
                     MnmD/FAD-dependent
                     5-carboxymethylaminomethyl-2-thiouridine(34)
                     oxidoreductase MnmC"
                     /protein_id="HCR5885622.1"
                     /translation="MKHYSIQPANLEFNAEGTPVSRDFDDVYFSNDNGLEETRYVFLG
                     GNQLEVRFPEHPHPLFVIAESGFGTGLNFLTLWKAFDQFREAHPQAQLQRLHFISFEK
                     FPLTRADLALAHQHWPELAPWAEQLQAQWPMPLPGCHRLLLDESRVTLDLWFGDINEL
                     TSQLDDSLNQKVDAWFLDGFAPAKNPDMWTQNLFNAMARLARPGGTLATFTSAGFVRR
                     GLQEAGFTMQKRKGFGRKREMLCGVMEQTLPLPCSTPWFNRTGSSKREVAIIGGGIAS
                     ALLSLALLRRGWQVALYCADEAPALGASGNRQGALYPLLSKHDEALNRFFSNAFTFAR
                     RFYDQLPVKFDHDWCGVTQLGWDEKSQHKIAQMLSMDLPAELAVAVEANAVEQITGVA
                     TNCSGITYPQGGWLCPAELTRNVLELAQQQGLQIYYQYQLQNLSRKDDCWLLNFAGDQ
                     QATHSVVVLANGHQISRFSQTSTLPVYSVAGQVSHIPTTPELAELKQVLCYDGYLTPQ
                     NPANQHHCIGASYHRGSEDTAYSEDDQQQNRQRLIDCFPQAQWAKEVDVSDKEARCGV
                     RCATRDHLPMVGNVPDYEATLVEYASLAEQKDEAVSAPVFDDLFMFAALGSRGLCSAP
                     LCAEILAAQMSDEPIPMDASTLAALNPNRLWVRKLLKGKAVKAG"
     gene            complement(55242..55520)
                     /locus_tag="OO357_000566"
     CDS             complement(55242..55520)
                     /locus_tag="OO357_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcL family protein"
                     /protein_id="HCR5885623.1"
                     /translation="MIAEFESRILALIDGMVDHASDDELFASGYLRGHLTLAIAELES
                     GDDHSAQAVHTTVSQSLEKAIGAGELSPRDQALVTDMWENLFQQASQL"
     gene            complement(55554..56102)
                     /gene="epmC"
                     /locus_tag="OO357_000567"
     CDS             complement(55554..56102)
                     /gene="epmC"
                     /locus_tag="OO357_000567"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P hydroxylase"
                     /protein_id="HCR5885624.1"
                     /translation="MNSTHHYEQLIEIFNSCFADEFNTRLIKGDDEPIYLPADAEVPY
                     NRIVFAHGFYASAIHEISHWCIAGKARRELVDFGYWYCPDGRDAQTQSQFEDVEVKPQ
                     AFDWLFCVAAGYPFNVSCDNLEGDFEPDRVVFQRRVHAQVMDYLTNGIPERPARFIKA
                     LQNYYHTPELTAEQFPWPEALN"
     gene            complement(56102..56911)
                     /locus_tag="OO357_000568"
     CDS             complement(56102..56911)
                     /locus_tag="OO357_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HCR5885625.1"
                     /translation="METFNSLFMVSPLLLGVLFFVAMLAGFIDSIAGGGGLLTIPALM
                     AAGMSPANALATNKLQACGGSISATIYFIRRKVVSLSDQKLNIAMTFVGSMSGALLVQ
                     YVQADVLRQILPILVICIGLYFLLMPKLGEEDRQRRMYGLPFALIAGGCVGFYDGFFG
                     PAAGSFYALAFVMLCGFNLAKATAHAKLLNATSNIGGLLLFILGGKVIWATGFVMLVG
                     QFLGARMGSRLVLSKGQKLIRPMIVIVSAVMSAKLLYDSHGQEILHWLGMN"
     gene            complement(56911..57735)
                     /gene="mepA"
                     /locus_tag="OO357_000569"
     CDS             complement(56911..57735)
                     /gene="mepA"
                     /locus_tag="OO357_000569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HCR5885626.1"
                     /translation="MNKTAIALLALLASSASLAATPWQKITQPVPGSAQSIGSFSNGC
                     IVGADTLPIQSEHYQVMRTDQRRYFGHPDLVMFIQRLSSQVSNLGMGTVLIGDMGMPA
                     GGRFNGGHASHQTGLDVDIFLQLPKTRWTSAQLLRPQALDLVSRDGKHVVSTLWKPEI
                     FSLIKLAAQDKDVTRIFVNPAIKQQLCLDAGTDRDWLRKVRPWFQHRAHMHVRLRCPA
                     DSLECEDQPLPPSGDGCGAELQSWFEPPKPGTTKPEKKTPPPLPPSCQALLDEHVI"
     gene            complement(57739..58824)
                     /gene="aroC"
                     /locus_tag="OO357_000570"
     CDS             complement(57739..58824)
                     /gene="aroC"
                     /locus_tag="OO357_000570"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HCR5885627.1"
                     /translation="MAGNTIGQLFRVTTFGESHGLALGCIVDGVPPGIPLTEADLQHD
                     LDRRRPGTSRYTTQRREPDQVKILSGVFEGVTTGTSIGLLIENTDQRSQDYSAIKDVF
                     RPGHADYTYEQKYGLRDYRGGGRSSARETAMRVAAGAIAKKYLAEKFGIEIRGCLTQM
                     GDIPLEIKDWSLVEQNPFFCPDPDKIDALDELMRALKKEGDSIGAKVTVVASGVPAGL
                     GEPVFDRLDADIAHALMSINAVKGVEIGDGFDVVALRGSQNRDEITKDGFQSNHAGGI
                     FGGISSGQQIIAHMALKPTSSITVPGRTINRFGEEVEMITKGRHDPCVGIRAVPIAEA
                     MLAIVLMDHLLRQRAQNADVKTDIPRW"
     gene            complement(58859..59791)
                     /gene="prmB"
                     /locus_tag="OO357_000571"
     CDS             complement(58859..59791)
                     /gene="prmB"
                     /locus_tag="OO357_000571"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5885628.1"
                     /translation="MDKIFVDEAVNELQTIQDILRWSVSRFSAANIWYGHGTDNPWDE
                     AVQLVLPSLYLPLDIPEDMRTARLTSSEKHRIVERVIRRVNERIPVAYLTNKAWFCGH
                     EFYVDERVLVPRSPIGELINNKFAGLISKQPQHILDMCTGSGCIAIACAYAFPEAEVD
                     AVDISPDALAVAEQNIEEHGLIHNVIPIRSDLFRDLPKVQYDLIVTNPPYVDAEDMSD
                     LPNEYRHEPELGLASGTDGLKLTRRILGNAADYLADDGVLICEVGNSMVHLMEQYPDV
                     PFTWLEFDNGGDGVFMLTKEQLIAAREHFAIYKD"
     gene            59957..60508
                     /gene="smrB"
                     /locus_tag="OO357_000572"
     CDS             59957..60508
                     /gene="smrB"
                     /locus_tag="OO357_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease SmrB"
                     /protein_id="HCR5885629.1"
                     /translation="MKKKTTLSEEDQALFRQLMAGTRKIKQDTIVHRPQRKKISEVPV
                     KRLIQEQADASHYFSDEFQPLLNTEGPVKYVRPDVSHFEAKKLRRGDYSPELFLDLHG
                     LTQLQAKQELGALIAACRREHVFCACVMHGHGKHILKQQTPLWLAQHPHVMAFHQAPK
                     EYGGDAALLVLIEVEEWLPPELP"
     gene            complement(60612..61502)
                     /locus_tag="OO357_000573"
     CDS             complement(60612..61502)
                     /locus_tag="OO357_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2544 domain-containing protein"
                     /protein_id="HCR5885630.1"
                     /translation="MSISEMKKWTIIFASLMLLVLSVVGASKSYAGDKLMSASFDSVR
                     VYYAMDKVTGAVASSVFNVTVITPKEVAYGKYDSFAYKGDTLRVISWSGSGSAPTLVL
                     TDFDTINNSNCPGIDTKIFRCAYMTFKITVASDDYGCPWIASFYSYTDLPGFGSYTAP
                     TVHNTICPTIPVASYDISWSENYVSHNKALRIQSTGSTVTTTLSTYLMEGGRLCDGSN
                     FSDNDGRGAYCRAVSELLTFTSYGCDKSTVTVTPTRHPVTDKVLHDIVVNVNTSSGQP
                     IDSTCRFQYVLNELKFVVLL"
     gene            complement(61489..62013)
                     /locus_tag="OO357_000574"
     CDS             complement(61489..62013)
                     /locus_tag="OO357_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5885631.1"
                     /translation="MKKKRTLFFISSLMLLGSGTTIAGDNLHFTGNLISKSCTPVING
                     SQLAEVHFPAIAASDLMNLGQSERVPLVFQLKDCHSSTLFNVKVTLTGTEDSALPGFL
                     AFDSSSSASGAGIGIETAAGTSVPINNTTGVTLPLNQGNNSLNFNTWLQAKSGRDVTS
                     GDFSATVTATFEYF"
     gene            complement(62010..62480)
                     /locus_tag="OO357_000575"
     CDS             complement(62010..62480)
                     /locus_tag="OO357_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5885632.1"
                     /translation="MKRISLILLWGFCSMALSNVSFHGYLVQPPNCTISNAQTIEITF
                     QDVLIDDINGSNYEQTVPYSITCDTAVRDPLMEMTLSWSGTPSDFDNAAVSSNITGLG
                     IQLKQAGQSFTINTPLVVNETDLPVLTAVPVKKSGVILPEADFEAWATLQVDYQ"
     gene            complement(62477..63025)
                     /locus_tag="OO357_000576"
     CDS             complement(62477..63025)
                     /locus_tag="OO357_000576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5885633.1"
                     /translation="MKSRAAKAEAGRASMKGSCSVWAVTGLLICCVGSGFAVAASKSV
                     SMNLRVLVDAPPPCTVNGAAVEFGNVFINKINRVDYKRPIDYSLVCNNLAMDDLRLQM
                     QATTVVINGETVISTGIPGFGIRVQKSSDHTILDLTSGSWLPFNFSSGVPVLEAVPVK
                     QSGTTLAAAEFNASATIVVDYQ"
     gene            complement(63030..63752)
                     /locus_tag="OO357_000577"
                     /pseudo
     CDS             complement(63030..63752)
                     /locus_tag="OO357_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416839.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
     gene            complement(63772..64494)
                     /locus_tag="OO357_000578"
     CDS             complement(63772..64494)
                     /locus_tag="OO357_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001433219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5885634.1"
                     /translation="MAQVDLSANYHEGQYTSAGLSLQGGATLTAHGGALHRTQNMGGT
                     RLLIDADGVADVPVEGNGAAVYTNMFGKAVVSDVNNYYRNQAYIDLNKLPENAEATQS
                     VVQATLTEGAIGYRKFAVISGQKAMAVLRLQDGSHPPFGAEVKNDNEQTVGLVDDDGN
                     VYLAGVKPGEHMSVFWSGVAHCDINLPDPLPADLFNGLLLPCQHKGNVAPVVPDDIKP
                     VIQEQTQQVTPTNPPVSVSANQ"
     gene            complement(64554..65708)
                     /gene="yfcU"
                     /locus_tag="OO357_000579"
                     /pseudo
     CDS             complement(64554..65708)
                     /gene="yfcU"
                     /locus_tag="OO357_000579"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher protein YfcU"
     gene            complement(65657..66412)
                     /locus_tag="OO357_000580"
     CDS             complement(65657..66412)
                     /locus_tag="OO357_000580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001435789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
                     /protein_id="HCR5885635.1"
                     /translation="MPDHSLFRLRILPWCIALAMSGSYSSVWAEDDIQFDSRFLELKG
                     DTKIDMKRFSSQGYVEPGKYNLQVQLNKQPLAEEYDIYWYAGEDDASKSYACLTPELV
                     AQFGLKEDVAKNLQWSHDAKCLKSGQLEGMEIKADLSQSALVISLPQAYLEYTYPDRD
                     PPSRWDDGISGIVADYSINAQTRHEENGGDDSNEISGNGTVGVNLGPWRMRADWQTNY
                     QHTRSNDDDEFSGDETQKNGSGVATMPGGRYHH"
     gene            complement(66494..67057)
                     /locus_tag="OO357_000581"
     CDS             complement(66494..67057)
                     /locus_tag="OO357_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5885636.1"
                     /translation="MSKFVKTAIAAAMVMGVFTSTATIAAGNNGTARFYGTIEDSVCS
                     IVPDDHKLEVDMGDIGAEKLKNNGTTTPKSFQIRLQDCVFDTQETMTTTFTGTVSSAN
                     SGNYYTIFNTDTGAAFNNVSLAIGDSLGTSYKSGMGIDQKIVKDTSTNKGKAKQTLNF
                     NAWLVGAADAPDLGNFEANTTFQITYL"
BASE COUNT        16791 a        18205 c        16722 g        15587 t
ORIGIN      
        1 gcgattgctg gtctgttgat gtagtggatt gtagttgctg acttttctta ccctcagatt
       61 tctgccccac ttgtcaattc aatctgccaa taaataacat aattatattt ttattcctca
      121 cagaaattcc tttttaaaaa tagcgaaccc actgtaaaaa acactttttg tatacctcta
      181 ccaagcgtaa taaaataaac gctcataatt cattataaat aataatgaaa tgacgccttt
      241 tattgataat ttacatatga aaaaattttc cttttgttat attatttctg ttttcactcc
      301 ctctgattat ttttaccaac cctgggtcac tgcattatcg ccaacgcccc gacacgtaag
      361 tcctgagcaa ttagaaaaaa cagttcgtta tcttacacaa actgtgcatc cacgtaatgc
      421 cggcaatatt gataatctga ataggtccgc agagtatata aaagagttct ttgtcagtag
      481 cggtgccaga gttaccgcgc agggcgtccc cgattggcta ttacgactct gcgcctggaa
      541 gccagaatta cccttacccg gcaatgtcct ggctttatcc cgatcgggga gactttattg
      601 ctgtggtcgg cagaatgcag gatatcaacg ccgttcgtca ggtaaaagcg gcattgttgt
      661 catcccggga tttatctgtt tattctatga atgccccagg gtttattccc ggtattgatt
      721 tttctgatca cctcaattat tggcaacacg atattcccgc cgtaatgatt actgacaccg
      781 ctttttatcg taataaacaa taccacttgc ccggtgatac cgcagacaga ttgaattatc
      841 agaaaatggc tcaggtggta gatggtgtta taaccttgtt atacaacagt aaataataaa
      901 aatttctcga ggcgggtcca gaaacagcga catgtacatt ttattttccc tcgtctgttt
      961 taagatgcga aacataaaat tcaatggagg aaaaataaat gggaatgatt ggctattttg
     1021 cagaaattga ctcagaaaag attaaccaac tgcttgagtc tactgaaaaa cctttgatgg
     1081 ataatataca tgacacgctt tcaggattac gccgactgga atcactggat cgaagtgagc
     1141 tgcgtaaaca gttttccatt aagcgactca atgaaatgga gatttatccg ggagtgacct
     1201 tttcagaaga gttagagggg caactctttg cctcgatcat gcttgatatg gaaaagctga
     1261 ttagcgcgta tcgacgtatg ctgcgacaag ggaatcatgc gctgacggtt attgttgggt
     1321 gatggtgtca gagcgccaaa aaaaaccgcc gaacatgtcg gcggctttct gacttacaaa
     1381 ctaacagttt acatcagcgg cattgctaac cgcacaatac taatcagcgg ttgtggccat
     1441 acacccagca ccagtaccaa cagtgcagaa atcagcacca cgataccgcc cgcgctgtac
     1501 tgccagtttg acggtgcatc gcgacccggt tgttccggtg cgtgcaggta caggctcacc
     1561 gccacgcgca ggtagtagta gaggccgatt gccgaaccga caaccacggc acccaccagc
     1621 caccacaagt gtgcctggac accgaccgcc agcacgtaga acttaccgat aaagcccagc
     1681 gtcatcggga taccggccag agacagcatc atcaccgtca tcactgccgc gaggatcgga
     1741 cgatgccaga acagaccgcg gtaagagaac agggaatcag catccgggcc acgatacggg
     1801 ctggacatca ggctgaccac gccgaacgcg ccgaggctgc tgaacagata accggccagg
     1861 taaaccccta ccgcttccat cgacatctcg ccggtttgca gcgcaatcag cgccaccagc
     1921 agatagccga ggtgagagat agatgagtaa ccgagcagac gtttgatgtt ggtctggctc
     1981 aacgccatca ggttaccgaa gatgatggag gcaaaggcga taatcgccag tactacgcga
     2041 atcgcttcgc tgtcacccac cggtgcgtag aggaacagac gcatcaccac accgaagata
     2101 gcgattttgc tcgccgtcgc caggaaagtg gaaaccggcg caggcgcgcc ctggtatacg
     2161 tctggcgtcc acaggtggaa cggcaccaga gagagtttga agccgaggcc aacaatcatc
     2221 atgccgaaac ctgccagcaa cagcggctcg ttgagcatac cgtcgccgag gtttttaccc
     2281 agcgcgacaa acgacaggtc gccagactgc gcatacacca gcgccatacc gaacagcagg
     2341 aaagaagacg ctgcggcaga aaggatggtg tatttgatac tggcttccag cgaacgtttc
     2401 tggcggaaag cgtaaccgac caggccaaac agcggcaaag agatcagttc aataccgagg
     2461 aacagagacg ccagatggtt tgcattcgcc agcaggatcc cgcccagcgc ggcaattaac
     2521 accaacaggt agaactcatc cttgttgtcg ttatagcctt caagccacgg gtaggcgaaa
     2581 gtacaggtgg cgaggctcgc caacaatacc agcccggtgt aaagcatggc gaaaccatca
     2641 acgcgcatca gcggcgtaac gtccatagcg cccgcctggc caacaaacca gagcgaaacc
     2701 agcgccgcgt taagcccaat aaccgagagc gtagcgttga ggaaatgatt gcgtcgccac
     2761 gcaatggaga gcatcacaac caccaccgtc aagccgacga tcagcaacgg tagcagtgcg
     2821 atcaggtttt gtggagttat tgtcatggcg atttacggcc ttgtagtagt aacggaatta
     2881 acaaaccact gctggatatt gccaatcgcg gagtgcgagg tatccagaat cggctgcgga
     2941 tagaagccca gcagtaccag cagcaccacc agcaacagga tcataaacag ctcacgcagc
     3001 gacatccctg gcagttcctg gctggcaatc tggcttttcg ctttaccgaa gtaagcgcga
     3061 tgcaacatcg ccagcgaata aacagatgca aagaccagcc caaaggtaga gataacggta
     3121 atcaccggga caacctggaa gctgccgaac agaatcataa attcgccgac gaagttaccg
     3181 gtgccaggca tcccaagcgt tgccaccgca aagaacagcg acagtgctgg cagccatttc
     3241 atcttgctcc acagaccgcc catcatgcgc atgtcgcggg tatggatacg ttcataaagc
     3301 tgaccgcaga gaataaacag acccgccgcc gacaagccgt gcgcaatcat ctggattacc
     3361 gcgccctggt aggccaactg gctgccggtg tagattgcaa tcagcacgaa gcccatgtgg
     3421 gaaaccgagg tgtaggcgat cagacgtttg atatcggtct gagcgaaggt catccacgca
     3481 ccgtagaaga tgccgataac acccagccac atagcgattg gcgcgaactc tgccgacgcg
     3541 ttcgggaaca gcggcaggga gaaacgcaac aaaccgtaag cggcggtttt cagcaagatc
     3601 cccgcgaggt ccacggaacc ggcggtcgga gcctgggagt gcgcatccgg cagccagcca
     3661 tgcagcggaa ccaccggcat tttgactgcg aaggcgatga agaagcccag catcaacaga
     3721 tattccacac cactggacat tggcgtattc agcagctctt catagttgaa ggtccagacg
     3781 ccggtcgcat tgtagtgaac aaaaaccagc gccaggatgg cgatcaacat caccagacca
     3841 ctcgcctggg tgtaaatgaa gaacttggtt gccgccgtga tacgcgtttt accgtcagag
     3901 gctttatgcc cccacagtgc gatcaggaag tacatcggca ccagcatcat ttcccagaag
     3961 aagaagaaca ggaacatgtc gatggcaagg aacacgccga taacgccgcc caggatccac
     4021 atcaggttga ggtggaagaa gccctgatat ttttcgatct ctttccacga acatagtacc
     4081 gccagcacac cgagcagacc ggtcagcacg accatcagca gcgacagacc gtcaatggcg
     4141 agatgaatag agataccaaa acgcgggatc cacggcatgt cgaattcaga ctgccactgc
     4201 ggaattccgg cggattgcgt cagtgaataa ccgccctgca accacagttg cagcgacagc
     4261 gccagcgtca atcccatggt gatcagcgcg atccagcgtg gcaccttgac gccaaagcgt
     4321 tcggtctgcc agcacaggaa gccgccaata aagggaatta atattagcca gggtagtaac
     4381 atggcgatct ttattccttg taaaagtccc gtcaggaccg gattttcaac gaattctcac
     4441 gacagaaaat tctcttcgga ctgggggtag tgtcggatgc gccgcgaagg cagcttatcc
     4501 gacctacgtt cttatatgcc caggggcaat cccacaatcc ttaactcaac gcagtaccat
     4561 caacagtgcc agcaccacga ccgcaccgat gctcatggat gccacatacc agcgcagata
     4621 accgttctcg cttaacagca gacctttacc tgcaaagcgg gaaagaaccg ccgggatgtt
     4681 catcattgag ttcagcggat cgcgtttcag caaccaggca atacccagga acggcttgac
     4741 gaacactttg tcgtacagcc agtcaaagcc ccaagcgttg taccaccagg tactcagcag
     4801 gcggcccggc gcactgttgg cgatggaggt caccagagaa cgtttaccca gccacagcca
     4861 ggctgccagc agaatgccga ccaccgcgac cacgccagag gtaatttcca gggtcaacat
     4921 gctgccgtgc gccagttccg tcgtttgcgg aagcacgccc tgcagcggcg gtacaatcag
     4981 tgcgccaacg aaggtggaaa ggatcagcag cacaatcagc ggcaggctgt gagttacccc
     5041 tttcacggcg tgagcgtgaa tttgttcttt tccgtggaag acgatgaaaa tcatacggaa
     5101 ggtgtagagc gaggtcataa acgcaccgac cagacctgcc accatcagat tgatatgacc
     5161 attcgccatc gcacccgcga ggatctcatc cttactgaag aagcccgcag tgaccagcgg
     5221 cagtgccgac agtgctgcgc cgcccaccag gaagcagaga taaaccagcg gaatagattt
     5281 acgcagaccg cccatcttga agatgttctg ttcgtgatgg caggccagaa tgacggaacc
     5341 ggatgccagg aacagcagcg ctttaaagaa cgcgtgagtc atcaagtgga aaatcgccgc
     5401 atcccatgcc tgcacgccaa gcgcgaggaa catgtagcca atctggctca tggtagagta
     5461 agcgagaaca cgtttgatgt cggtctgtac cagcgcggca aaaccggcca gcagcagcgt
     5521 aaccgccccg acaataccca ccagatgcag aacttccggc gtcatcagga acaggccgtg
     5581 ggtacgggcg atcaggtaga cacccgcggt taccatcgtt gcggcgtgga tcagcgcgga
     5641 gacaggcgtc gggcccgcca tcgcgtcagc aagccatgtc tgcaacggca actgcgcaga
     5701 tttaccgacc gcaccgccca gcagcatcag cgtcgcccac atcagcatgt tattgccgtc
     5761 agcaaagtgc gctggtgcca gttccaccat atcgcggaag ttcagggtgc ccagttcgtt
     5821 gtaaagaatg aacagtgcga aagcgaggaa aacgtcaccc acacgggtca cgacgaacgc
     5881 tttcattgct gctgcgccat tcttcggatc ggtgtaatag aaaccgatca gcagatagga
     5941 gcacaggccc acgccttccc agccgaggta catcagcagc aggttatcgg cgagaaccag
     6001 aactaccatg ctggcgatga acaggttggt gtaggcgaag aagcgagagt agccctcttc
     6061 accgcgcatg taccaggagg cgtacatgtg aataaggaaa cccacaccag tgaccaccga
     6121 gagcatggtc agcgacaggc cgtccagcac caggttaaaa ccgatgttaa agtcgcctac
     6181 cgacatccac gtccacagcg gctggctgta tgcctgctcg ccgttagcga agaaatcaac
     6241 gccgataaag gcggttacca gcgccgccag gcccacagag ccaacgccga cgatcgccga
     6301 aacgttttca gaccagcgcc cacgggagaa cgccagcagg acgaagccaa tcaatggcaa
     6361 aataatggtt aaggcaagca tgttcatccg cgcatctcac ttactgaatc gatgttcagg
     6421 ttctggcgac gacggtgaag ttgcagcagc agcgcaaggc cgatactcgc ttctgccgcc
     6481 gcgaggctga tggcgagaat gtacatcacc tgaccgtcgg tctgacccca gtagcttccg
     6541 gcgaccacaa aggccagcgc ggaggcgtta atcatgattt ccagaccaat cagcataaac
     6601 agcagattgc gacggataac cagaccggtt aagccaagaa cgaataagat tgccgcgagg
     6661 atcagtccat gttgtaaggg gatcatgcgt gctcctccgt ttttcttttc gcgctgtcgt
     6721 ctttacgatt gctcagcact tcacccgcac gctcttcacg accgacgtgg aaggccacaa
     6781 ccagacccgc gagcagcagc atagaagcca gttccaccgc cagtacgtaa ggcccgaaca
     6841 gcgtaatacc cactgcttta gcactgattg gcgtaccgtc gataccctga tcgttaacac
     6901 cgaggatggc gtaaacaatc accaccagca tgatggccga caaaattgcc gggccaatcc
     6961 acacctgcgg tttcagccac tggcgttcct gttcgatttc tgaaccgccc aggttgagca
     7021 tcatcaccac gaacacgaac agcaccataa tggcacccgc gtagacgata atttccagcg
     7081 caccggcgaa gtaagcgcct agtgagaaga acaccccgga aatcgccagc agcgaaataa
     7141 tcaggtacag cagtgcgtgt accggattgg tatgggtgat cactcgcaag gtcgcaagta
     7201 tggctatcag gccacagata taaaaagcga actccattgc ccctctcctt acggtaacag
     7261 gctcttgacg tcgataggct tggcttcgtt ctctgcttcg cccttatctt tgccgtcgat
     7321 tgccatacct gccatccggt agaagttata ttccgggtat ttgcccggac cggagatcag
     7381 cagatcctct ttctcgtaaa ccagatcctg gcgcttgtat tcccccattt cgaaatccgg
     7441 ggttaactga atcgccgtgg tcggacaggc ttcttcgcac agaccacaga aaatgcagcg
     7501 tgagaagtta atgcggaaaa attccggata ccagcgaccg tctttggttt ctgctttttg
     7561 cagcgagata cagccgaccg ggcaggctac cgcacagagg ttacaggcta cgcaacgctc
     7621 ttcgccgtcc gggtcgcggg tcagaacgat acgaccacga taacggggcg gcagatagac
     7681 cggctcttcc gggtacattc gcgtttcgcg tttggcgaac gcgtgcaggc cgatcatcca
     7741 gatactacga acctgggtgc cgaaacctac taacaattct tttaaggtca tggtcttatt
     7801 gccccttatt gcgcctgcca gagaatgaca gccgccgtta ccagcaagtt gatcagcgtc
     7861 agcggcaggc agattttcca gccgaaggac attacccggt cataacgcgg acgcggtaac
     7921 gacgcacgaa tcaaaatgaa catcatcata aagaacgcgg ttttcagcgc gaaccagatg
     7981 aatggcggta acaacgggcc ttgccagcca ccgaagaaca gcgtcaccat caatgcagag
     8041 atggtcacaa tcccgatgta ctcacccacg aagaacagac cgaacttcat accggaatat
     8101 tcaatgtggt aaccatccgc cagttcctgc tcggcttccg gctggtcaaa cgggtgacgg
     8161 tgacacaccg ccacgcccgc gatggcaaag gtaataaaac caaagaattg cgggatgacg
     8221 ttccacacat gcgcctggct gttgacgatg tcggtcatgt tgaatgaacc ggcctgcgcc
     8281 accacgccca tcagggaaag cccgaggaac acttcgtagc tcagggtctg cgcagaagca
     8341 cgcatcgcac ccagcagcga gtatttgttg ttacttgacc agcccgcaaa cagcaccgcg
     8401 taaaccgcca gacctgccat catcaggaag aacaaaatcc cgatgttcag gtcggcaacc
     8461 acccaacccg gactgactgg cacaattgca aaggccagca gcagcgaggt aaaggcaatc
     8521 atcggtgcca gggtaaagat gacgcgatcc gagaatttcg ggatccagtc ttctttaaag
     8581 aacattttga tcatgtccgc aaccagctgg agcgaaccgc cccagccaac acggttaggt
     8641 ccataacggt tctggaacag acccagcagg cgacgttcgc caaagctcat gaatgcccca
     8701 caggtgacaa ccaccagcag gatcaccacc gctttgagga tggtcagcag gatctcaatc
     8761 agttccggtg atatccaact cattgttgtg cctccttgag atcctcaaga tgcgcgccag
     8821 ccagcaccgg agcaatgccg gacataccca tcggcaagcc cacctgccct gccgtcagtc
     8881 cttcggcgat ttcaaccggc agcgtgaccg tgttgccatc gtaactaaag gagacgcgtg
     8941 tacctgcgtt cacacccaac ttcgcggcat ccgctgggtt gagtttgatg tacggctgcg
     9001 gcatacggct ctggaagacc ggagcacgct gtgacaattc atcgctgcca aacaggtggt
     9061 agtacggcgc gatacgccat ttcccgtcct gcggctggaa gcgtgccggt acgctggtga
     9121 agtaatccag accgttttcg ctggtttcaa acagacgcac gcccggatcg ccaaagcgca
     9181 gtttgccgcc cacttcgtcc tggaatttgt tccacgcctg cggggagttc cagcccggcg
     9241 cccaggcaaa cggcacttgc gaacggtgcg cggtcggctg gttgttacct tccatcgaga
     9301 aggtgaacat ggtgtcaata tcctgcggct gacgcggctc atgaacgctg atattggcgc
     9361 gcatggcggt acgaccgctg taacggtgcg gttcacgggc cagtttctgc ccacgaatac
     9421 ggaatgtcgc atccggcgca gcatctttga tacccgccag ttctgggatt ttcgccacaa
     9481 cagcgtcaat tacatggtcg agctgcgtcc agtccacttc acggctcagc aaggtgctgt
     9541 gcagcgagtg cagccagcgc cagctttcca gcatgacagt tttgctgtcg taataagccg
     9601 gatcgtaaac ctggaagaaa cgttgggcgc gaccttcatt attaattacc gtaccgtcgc
     9661 tttcagcaaa gctggcagag gaaagcacca gatgggcgtt ttccatgatc gctgtgcgtt
     9721 gatgatcaac caccatcacc agcggcgctt tagccagcgc agcattcacg cgggtagcag
     9781 aagcgtaacg atgcagatcg ttttccaaca ccaccaccgc gtcggcgcgt ccggtttcca
     9841 gttcggttaa cgcttcttca agcgaaccgc cacccataat gcccagcccc atgctgttga
     9901 cggaacgggc aatcatggtg ataccaacgt cagcgccgcg acctttcagg gctttcgcga
     9961 cgttagccgc agcctgaatc acctctatgc taccggcgtt cgtcccggag ataatcaacg
    10021 gtttcttcgc acctgccagt gcctgcacga tgacgtcgat tttgttttgc agctcaggtt
    10081 cgataccgtc aaccgctggt gcggagttat ccagcgcatg ggcgatggca aaacctaaac
    10141 gcgcctgatc ttcaaccggt gcgcggtaag tccacgccgc gatatcatcc agacgagtgt
    10201 catcaacgtt ggtaacaaac agcggatgct tcgcacgttg accgatgttg aggattgccg
    10261 caatctgcca gtcagccact ttctgtgctg cagccatttc gcgcgcttta cctttcaccg
    10321 cctgacgcac tgccagtgcg acgcgcgcgc cggtctgggt aacgtcttcg cccagcacca
    10381 gtaccgcatc gtaagattcg atttcgcgca gagccggagt ataaatgccg ccttcacgca
    10441 gcactttcag cgccagttgc agacgttcct gctcaccgtg agcgataccg gtgtagaagt
    10501 tttcttcgcc caccagttca cgcagcgcaa agttgctttc cacgctggca cgcggagaac
    10561 caataccgat cactttcttc gactgacgca gaatatctgc cgcgccctgc attgcctgtt
    10621 cggcgttgag ggtaatgaaa tcatcgccac gacgctgtac tggctgacgc ggacgatcct
    10681 tcaggttgac gtaaccgtaa ccgaaacgac cacggtcgca gaggaagtag tggtttaccg
    10741 taccgttgta acggttttcg atacgacgca gttcgccgta acgttcaccg gggctgatgt
    10801 tacagccgat ggaacattgc tggcagatgc tcggcgcaaa ctgcatatcc catttacggt
    10861 tgtaacgctc ggagtgcgtt ttgtcggtaa atacgccggt cgggcaaatt tcgaccaggt
    10921 taccggaaaa ttcgctttcc agcgtgccgt cttccgggcg accgaagtag acgttgtcgt
    10981 gcgcgccata aacgcccaga tctgtaccgt cagcgtaatc tttgtagtaa cgcacacagc
    11041 ggtagcaggc gatgcagcgg ttcatttcgt gagagatgaa tggccccaaa tcctgattac
    11101 ggtgggtacg tttggtgaaa cggtagcgac ggaagctgtg tccggtcatc acggtcatat
    11161 cctgaagatg gcagttaccg ccttcttcac ataccggaca gtcgtgcggg tggttggtca
    11221 ttaaccactc gaccacactt tcacggaact gtttcgcttc ttcgtcgtca atggaaataa
    11281 aggtgccatc ggaagccggt gtcatacagg acatcaccag gcgaccacgc gtgtcttccg
    11341 cgttttggta ttgcttcacc gcacactggc ggcaagcacc gacgcttccc agcgccggat
    11401 gccagcaaaa gtaaggaata tcaaggccca gagacagaca agcttccagc aggttgtccg
    11461 ctccgttgac ctcgtattct ttgccgtcta catgaattgt agccattagc atgcttccag
    11521 ttttctcggt cagggaccga gcgttaatcg aaattcggtt accagcgctc tttcagcagg
    11581 ttcggctgaa tcccattaat caaatgggta ttgctgaacg gctgtttgat tcccgcctca
    11641 aattcttcgc ggaaatattt gatggcgctc tgtaacggct ccactgcacc aggtgcgtgg
    11701 gcacagaaag ttttacccgg gcctaagaat cgacacagtt gctcaagtgt ttcgatatcg
    11761 cccggctggc cttcgccacg ctccagcgca cgcagaattt tcacgctcca cggcagaccg
    11821 tcgcggcacg gcgtacacca gccgcaggac tcacgggcga aaaactcttc caggttacgc
    11881 accagcgaca ccatgttgat ctcatggtca accgccatcg ccagcgccgt acccagacgg
    11941 ctgcccgctt taccgatact ttcgaattcc atcggcagat caaggtgcgc ttcggtcagg
    12001 aagtcagtcc ccgcgccgcc tggctgccag gctttaaatt tcagaccatc acgcatacca
    12061 ccggcgtaat cttcgaggat ctcgcgtgcg gtggtgccga acggcagttc ccacagtccc
    12121 ggatttttca cccgaccgga gaagcccatc agcttggtgc cagcatcttt acttttcgag
    12181 atgttctgat accactccac gccgttagcg aggatcgccg gaacgttaca cagggtttcg
    12241 acgttgttga cacaggtcgg tttgccccat acgccggagg ttgccgggaa ggggggtttc
    12301 gagcgtgggt tagcacgacg cccttccagg gagttgatta acgctgtttc ttccccgcag
    12361 atgtagcgcc ctgccccggt atggacgaac agttcgaagt cgaaacctgt tcccataatg
    12421 tttttgccaa gcaagcccgc ttcagtggct tcggcaatgg cgcggcgcag atttactgcc
    12481 gcttcgatat attcgccacg caggaagatg tagccacggt aagctttcag cgcaaacgcg
    12541 gagatgagca taccttccac cagcaggtgc ggcagttgct ccatcaacag gcggtcttta
    12601 taggtgcccg gctccatttc atcggcgtta cacagcaggt aacggatgtt catggattcg
    12661 tctttcggca tcaggctcca cttcaggcca gtcgaaaagc ccgcgccgcc gcgccctttc
    12721 agaccagcgt cttttacctg attaacgatt tcgtccggag acagcccggt cagcgcctta
    12781 cgcgcccctt cgtaaccgtt tttgctgcgg tattcgtcca gccacactgg ctgtttgtca
    12841 tcgcgcagac gccaggtcag cggatgcgtt tcgggagtac ggataatgtt tttcatttat
    12901 accgctccag cagttcaggg atcgcttccg gggtcagatg cgcgtgagtg tcctcatcga
    12961 tcatcatgtt tggcccttta tcacagttcc ccaggcagca agttggcagc agcgtaaagc
    13021 ggccatcaaa tgtcgtttgc cctggtttga tgttcagctt tttctcgagc gccgcctgaa
    13081 taccctgata cccgttgata tgacagacca cgctgtcgca ataacggatc acatggcgac
    13141 caaccggctg gcggaagatc tgactgtaga acgttgccac accttcgacg tcgcttgccg
    13201 gaatacccag cacatcggcg atcgcgtgga tcgcaccatc cggcacccag ccacgctgct
    13261 tctgaacgat tttcagcgct tcaatggacg ccgcacgcgg gtcttcgtag tggtgcatct
    13321 cgtgctcaat cgcttcacgc tctgccgcac tcagctcaaa agcctcggtt tgtggttgtt
    13381 gattctcgtg cataattagc ggtccacatc tgacataaca aaatcgatac tgcccagata
    13441 aacaatcagg tcagacacca ggctgccgcg gatcgccgcc ggaatttgct gcaaatgcgc
    13501 ataactcggc gtgcggatac gagtgcgata actcatggtg ctaccgtcgc tggtcaggta
    13561 gtaactgttg atccccttgg tcgcctcaat catctggaaa gattcattgg caggcatcac
    13621 cggcccccac gacacttgca ggaagtgggt gatcagggtt tcgatatgtt gcagcgtgcg
    13681 ctctttcggc ggcggcgtgg tcagcgggtg atccgctttg aacgggcctt ccggcatgtt
    13741 gttgaggcac tgctcaagaa tgcgcagact ctggcgcagc tcttccactt tcagcattac
    13801 gcgggtgtag cagtcagaaa caccaccacc aaccgggatt tcaaagtcga agttttcata
    13861 gccagaataa ggacgcgcct tacgcacgtc gaagtcgatc ccggtagcac gcaggcccgc
    13921 gccagtggtg ccccactcca gcgcctcttt cgcgccatag gcggcaacgc cctgggaacg
    13981 acctttcaga atggtatttt gcagcgccgc tttctcgtaa gacgccagac gtttcggcat
    14041 ccagtcgagg aactcacgca gcagacgatc ccagccgcgc ggcaggtcgt gcgctacgcc
    14101 gccaatacgg aaccacgccg ggtgcatacg gaaacccgtg atcgcttcca ccagatcgta
    14161 aattttctga cgatcggtaa aggcgaagaa cacgggcgtc attgcgccga cgtcctgaat
    14221 aaaggtcgag atgtacagca ggtggctgtt gatacggaac agttcggaga gcataacgcg
    14281 aatgacgtta acgcgatccg gcacggtgat cccggccagt ttctctaccg ccagcacgta
    14341 aggcatttcg ttaacgcagc cgccgaggta ttcaatacgg tcggtatacg gaatgtagct
    14401 gtgccaggac tggcgctcgc ccattttctc cgcaccacgg tggtggtagc cgatgtctgg
    14461 tacgcagtcg acaatctctt cgccgtcgag ttgtaaaacg atacggaaag ccccgtgcgc
    14521 cgacgggtgg ttcggaccga ggttgaggaa catgaagtcc tcattttcgg tgccgcgctt
    14581 catcccccac tcttccggtt tgaaggtcag ggcttccatc tccagatcct gtttggcttt
    14641 ggtcagctca aacggcgaga attcggtagc gcgtgccggg taatctttac gcagcgggtg
    14701 acctttccag gtttgcggca tcatgatgcg gcgcaggttc gggtgaccgt cgaaagtaat
    14761 gccaaacaga tcccaggttt cacgctcata ccagttagcg ttcgggaaca gtttggtgaa
    14821 ggtcggtacg tgcaggtcgt tttctgccag cgccaccttc agcatgatgt cgcggttacg
    14881 atcgatagaa atcagatggt agaaaacgga aaaatccgcg gcaggtaacc cttcgcggtg
    14941 tgtgcgcaga cgttcgtcca tgccgtgtaa gtcaaacagc atgacgtaag gtttcggcag
    15001 tttctttaag aaatcgccaa cttccagtaa ttgttcacgc ttgatccaca caacgggaac
    15061 cccggtgcga gtcgcctgaa cagtaaaggc atccggccca aaacggttgc gcagttcgcc
    15121 aattaccgga tcatcaagat gatcgcgggt ctgccaggcg ggttcttgcg cggttaagtc
    15181 ggtcatattg ttcaccattg caaatggtcc gtggtgactg tcggcgcagt aagcttcgcg
    15241 aaatagattt aggaataagc gaagaaaaat ccctttgccg acaggcgcaa attaaatctc
    15301 gtcaggtgta cgcaggttgg ttacggcaat gcgttcaccg cgcttgcgtt cgcgctctga
    15361 ttgcatattg gcgcgataaa cgccctgatc gccaaccacc caggagagcg gacgacgttc
    15421 tttgccgata gattcctgca acagcatcag tgcctgcatg tacgcttcag gacgcggcgg
    15481 gcagcccggg atatacacat caaccgggat gaatttatcg acgccctgca caacggaata
    15541 aatatcgtac ataccaccag agttggcaca ggcacccatt gagataaccc attttggttc
    15601 cagcatctgg tcatacagac gctgaataac cggtgccatt ttggtaaagc aggttcctgc
    15661 aaccaccatc aggtcagcct gacgcggcga agcacgcaat acttctgcgc caaaacgcgc
    15721 cacgtcatgc accgcggtaa acgaagtcac catctcaacg taacagcagg aaagaccgaa
    15781 gttatacggc caaattgagt ttttacgacc ccagttaacc atgtcattga gcttgcccat
    15841 aaacacgttt ttgttaactt cttgctccag agggtcggtt acgatctcct gcttttgcag
    15901 ggggtaacgg tcgttctcac cgttgggatc tatgcgggtg agcgtataat ccatcttaat
    15961 gcctcgcggt tagcgttgac gattagcgat actgttcgtt tccgggttca tacgctcgcg
    16021 gcgtgaacgc gcgggcgtcc agtccagcgc gccaatacgc accagataaa ccagacctgc
    16081 cagtaacaca aaaataaaaa ttgcagcttc cacaaagcct acccagccgc tttcgcggat
    16141 agaggttgac catgcgaaca gatacagcgc ttcaacgtcg aagataacga agaacatggc
    16201 caccagataa aacttggcgg acaggcgtaa gcgggcggag ccgaccgagt cgataccgga
    16261 ttcaaacggc acgtttttcg acctcgcgcg tgcgcgaccg cctaaaaacc aaccgcctac
    16321 cagcatcagg caacacaggc caatggcaac gataagaaag atagcgaatg cccagtgatg
    16381 agcgatgact tcagtggatg ttgacatact cattgcttac tcatcaaaag tagcgccaga
    16441 ttcactgctc ttcacggcag atggacgcca catcgattca tggggaggaa taaaaaaaac
    16501 cttacaataa ctgtagaaat tcttttatac agctaattga tgtggtcttt tactcctttc
    16561 tataaccttt tgtcaacttt aacaaaagct tcttcacatt agtttacata atatcaacac
    16621 cattagcatt taatgccctt tcaccccaga tccttgacga ctccaggata attagatgtt
    16681 gttgaatcgt gtccgttgtg aagcaatgga aaaaatacgg gtctattttg acaggaattt
    16741 gtgtcgattc ctccccccaa aagagagtat tttcttgatc tgtgacacgc ttttgttatt
    16801 ccataacaaa aacgcagcaa caaatttacg tattttttaa catcattgta gcaggtgatt
    16861 tttttcaggc gattatttgt gcattcggga cgtgaatctc tggtgattga aaaataaaca
    16921 gttttgtacg ttctgcacta tgcgaggaag gatttttact aaaaaaaagc cgctggggtt
    16981 taaaacaccc ccagcggctc gttttttaca ctattgtctc aggaattatc tatcgtccgt
    17041 cgattactcg atatcccttt caatcaacaa tgaatcatcc ccttccggag cagacattgg
    17101 gctgtattgc cacggattat ggtagctctc catcgcctga tagatcacct gggccagctc
    17161 attattactg gacgggtcat agcacagcaa atattcggta tcaggtagcg gcggtaagcc
    17221 atctactcca ctcagcacac gcaggtccgg gctcatcatc tcaaccggcc ttgccgtcac
    17281 gccaagaccg gctttcactg ccgcacgaac ggccggaagc gtcgaggcga cataagccag
    17341 tcgccatgga atatctactt tattaagcgt cgccagcacc atatcgcgaa acggacttgg
    17401 atcatccagc aatacaagag ggatcggctc gcctttttgc aatacgtatt ccgctgcgca
    17461 gtaccagtgt gttggcgagg tacgcaggtt caatgcctta aacgctgaag gacgatgggt
    17521 ggttaccatc aaatccactt cctgggattc cagcatttct gccatatagg cattacgttt
    17581 aacgcggaca tccagcgcta atttcggata aaccgaactc acgcgattta acaggaaagg
    17641 taagatcgta tcggcagatt catctgaagc accgatagtt aacacgccct gaagattact
    17701 gaacattaat gatgagcagg cctcatcatt aaaacgcagg attttcctgg cgtaaccaag
    17761 aagttgaatg ccatgttcag ttaacagttt gttgcgaccg tgacgagcga acagttcttt
    17821 cccaacgagt tgttccagac gctgcatttg ctgacttacg gcggactgag tacgacacac
    17881 agcggcagct gcggcagcaa aagtgttcag atcggcaaca gcaacaaatg ttctcagcag
    17941 atcgaggtcg aggttaatta tcggacgatt tgcacttatc atatattatc acttactggc
    18001 ggctcatact gagctggtta atgctgtgca cacacaaaca agcaattcca tttgtaatgt
    18061 gcctccctgg cagtttcatc cggaacccgg acgaaagtaa aaatgcatat gagttgcact
    18121 aaaaaagcga ctcacattgt tccgttataa tgcctgaagt agatcacaga atatatcttc
    18181 agggatcgca tatctattaa gttactcact cttttctatt tatgacatgc gcgtgtttgt
    18241 ataaatgtaa atgtgagtcc ttgttccact ctcgtgcagc atcgctggtc atacgcgaac
    18301 acgtacaaac agcaatggtg aagcggcatc aagagcaggg atccgtcatt tatgcgagca
    18361 ttttacccca aaaacctttt atttataagg gtcattgcga attatctgat gcaaagttat
    18421 gttatgttag gcaaagtaat cttctattat taataagcac atcaaaacct ttttgaatat
    18481 taaataataa ttaattagca tcatcctcat tcattaattc cgcttaacaa tagtttcaca
    18541 ccttaccccc attgcgacac ctcggaaatc atcacgctgt taaactctcc gtaatgcgga
    18601 aatatcatca tcgcgtttca ttagtgaatt cttaaatgag gcattttcac acaattatct
    18661 tacagataaa aaaaccagac ttgcaattaa gaattagaac aattcactat ataacattgc
    18721 atgtaaagca tataagcctc attatttgtc attattaagt tattaacagc acaatcgagc
    18781 cttcccctct ggcaaaatct tattctgcag accttcaaaa caccgtcctg ggggagtaca
    18841 ttgttctaag ctgacttcca cggcagggag tggcgataac agcaaaaaag gtcaagattc
    18901 atgtccccca ttgaaaaatc cagcaaatta gagaatgtct gttatgacat ccgtggtccg
    18961 gtgctgaaag aagcaaaacg cctggaagaa gaaggtaaca aggtactgaa actgaacatc
    19021 ggcaacccag ccccattcgg ttttgacgcg ccagatgaaa tcctcgttga cgtgatacgc
    19081 aacctgccta ccgctcaagg gtattgcgat tccaaagggc tttactccgc gcgtaaagcc
    19141 atcatgcagc actaccaggc tcgtggcatg cgtgatgtta ccgtggaaga tatttacatc
    19201 ggcaatggtg tatcggagct tatcgttcag gcaatgcagg cattgctgaa cagcggggac
    19261 gaaatgttgg ttcctgcacc agattaccca ctctggaccg cggcggtttc gctttccagc
    19321 ggtaaagcgg tgcattatct ttgcgatgaa tcctctgact ggttcccgga cctcgatgat
    19381 attcgcgcta aaattacgcc tcgtacgcgt gggattgtta ttatcaaccc aaataaccca
    19441 accggcgcgg tatattccaa agagctttta atggagattg tggagattgc acgtcagcat
    19501 aatctcatta tcttcgccga tgaaatttat gacaaaattc tctacgacga tgctgagcat
    19561 cactcaattg cgccgctggc acctgacctg ctgaccatta cctttaacgg actgtcgaaa
    19621 acgtaccgcg ttgcaggctt ccgtcagggg tggatggtgt tgaacgggcc gaaaaaacac
    19681 gccaaaggct acatcgaagg tctggaaatg ctggcttcaa tgcgcctgtg tgctaacgtt
    19741 cctgcgcaac acgccattca gaccgcgctg ggtggttatc agagcatcag tgaatttatt
    19801 acccctggcg gtcgtcttta tgagcagcgt aaccgcgcgt gggaactgat caacgatatt
    19861 ccgggcgttt cctgcgtgaa acctcgtggt gcgctgtata tgttcccgaa aatcgacgcc
    19921 aaacgcttta acattcatga cgatcagaaa atggtgctgg atttcctgtt gcaggaaaaa
    19981 gttctgttgg tgcaagggac ggcattcaac tggccgtggc cggatcactt ccgcattgtc
    20041 acgctaccgc gtgtcgatga tatcgagctg tctttgagca agttcgcgcg tttcctttct
    20101 ggttatcatc agctgtaatc ttaatttcac tgccggagat tgcatccggc agtgttatcc
    20161 cgccacaatg acctgatgat gtcatcatac gtaaggtcac tatgaaacag agccattttt
    20221 ttgcccatct ctcccgcctg aaactcatta accgctggcc gctaatgcgc aacgtgcgga
    20281 cggaaaatgt gtccgaacac agtttgcagg tagcgatggt cgcccatgcg ctggcagcta
    20341 tcaaaaatcg gaaatttggc ggtaatgtca acgccgaacg tatcgcttta ctggcgatgt
    20401 accacgatgc ctcagaagtg ctcaccggcg atctccctac tccggtgaaa tacttcaatg
    20461 cgcaaatcgc tcaggagtac aaggctattg aaaaaatcgc tcagcagaaa ctggtcgata
    20521 tggttccgga agaactgcgg gatatctttg cgccgttaat tgacgagcat gcatatagcg
    20581 atgaagaaaa atcgctggtg aaacaggcag atgcactgtg tgcatatctg aaatgtctgg
    20641 aagaactcgc ggccggaaat aatgaattct tgctggcaaa aacgcgactg gaagcgacgc
    20701 ttgaagcgcg tcgcagccag gagatggact acttcatgga agtatttgtt cccagcttcc
    20761 atctttcgct cgatgagatt agccaggatt caccgctgta agcagccgga gtctgcgtcg
    20821 catcagtcaa taagcgccgg atgcgacatc aggctcttgt caaaacggaa acagcatcgg
    20881 gatcatcacc acacaaaccg ccatcacgat aatggtgaac ggtaccccca acttcacaaa
    20941 gtcactgaag ctgtaatttc ccggacctaa aaccagggtg ttaacaggtg aagacaccgg
    21001 tgtcataaag gcggcggatg ctgccatcgc cacgaccatc gcaaatggat aaggcgacac
    21061 tcccatcgtt ttggcagcag ccagcgcaat cggagccatc aacaccgccg tcgcggtatt
    21121 agagataaac agcccaataa ccgccgacaa gacaaacaaa caccccagca tcatatgtgg
    21181 cccataaccg ccgccaatat ccatcagccc tttcaccgcc agcgcgacac ctcccgtttt
    21241 ctgtaatgcc acagcaaacg gcatcatccc aacgatcaaa ataatgctcg gccagtgaat
    21301 ggatttatag gcgctttcag catctataca gcggaatttc cccatcagca ggcaggcgat
    21361 gatagcggca acaggattag gaatttcatt tgtcagcatt aacgccacca ttagcaccag
    21421 acagaaaatg gcatggggtg cctggctgtg cgcgggcgat gcttcactca cctcttccgg
    21481 taagttcagc gctacgaagt cgcggccctg tttggccagc ataccgatca gtttccagtt
    21541 acccacaacc aggatgatat cgcccagcag cagaggctca tccgccagcg aaccttccag
    21601 cgccacgcca ttgcgcttga gccccaccac attcagtccg tagcgggtac gaaaaccaat
    21661 ttcgcgcacc gatttaccaa tcagttctga ctcaggaatt aatgaaatct ctgccatgcc
    21721 cacatcaagg gcctggtcag aaaaatactc gccgcgcagt accatcggct ccagcaattg
    21781 ctcactacaa aattgccgga gatcgacatc agccgcagac atatcaataa gcaaaacgtc
    21841 acgcgcgcga aattcagaaa ccccattaac gttcacgata acgcgacgaa aacgccgcca
    21901 gcgttcaaca ccgatgacgt tagcgccata acgctcacgt aatttgagat catccagccg
    21961 ttgacctatc attggcgatc cggggcgaat agccagacgt cgcgcacgcc cggtcagtcg
    22021 atattcacgg ataagatcgc gaaaggttcg acgcgtccag ccttcgcgct gcggggtctg
    22081 ggtatcccct ttcagcatga aacgcatcac taacatatac aaaataccca gcaccagcac
    22141 gaccaggcca ataggtgtta cgctaaagaa actgaagccg tgatagcctt cacgcagcaa
    22201 ttcactgttg actaccaggt tcggcggcgt cgccaccagc gtcatcatgc cgctaatcag
    22261 cccggcaaaa cttaacggca tcatcagacg tgacggcgaa gtttgcatac gcatggcaac
    22321 gcttaacacc acgggaataa agatagcgac aacgctggtt gaactcataa acgcgccaag
    22381 ccccgcgacg gtcagcatca acaaaaccaa cattttgatt tcactgttgc ccgccacttt
    22441 gaccagccat gttcccatta cggtggcaac accggtacgg accaaaccat cgccaataat
    22501 aaacaaggcg gcaatcagga caacgttagg atcagaaaag ccggaaaata cttcggggac
    22561 cgtcagcgtt ccgcttaatg caaacgcgac aataacaaac aaagcgaccg catccatacg
    22621 cactctgccc gtcgcaaaca agacgatggc aacggccagt aatgaaagaa cccaaatcaa
    22681 ttcaccgttc acaacttatc cttgttaatt gagggggatg acttgattct gccataaaaa
    22741 agccccgacg agacggggct aaatcatgat caggtgtttc actgaataat aacatcgcca
    22801 tttggctgtt tggtcacagt aatttgctcc agactgtgga ggaccaaatc gacctcattc
    22861 aggcgcgggg tatctgccgg agcgttaacc gcaatgacat ggcaacccgc cgccaggcca
    22921 gaaagcacgc cagcgggagc atcttccacc accacacact cctgcggcgc aagccccagc
    22981 agctgcgcac ctaacagata cgcatcaggt tctggttttc cgcgcttcac tcgctcagcg
    23041 gttacaaaca cctctggtgc gggaagcccc gctattttat ggcgcgctcg cgctaccggc
    23101 atggagccag aagtcacaat ggcccacgga atacctgctt tattcaaatg attgagtaag
    23161 gcgattgccc caggaagcgc ggtaatacct tcggtttccg tggcctcgat ttgctccaga
    23221 cgcgtaaact cggcggcaat atcagcctcg gatttgcccg ccataaaatg gcgcaaagag
    23281 gtgatcgcct gtttaccgtg aatgaaagcc agcacctctt ccggcgctaa cccatgacgt
    23341 ctggcccagt tgctccacgc ccgttctacc gcaggcaggg aatccaccag cgttccatca
    23401 agatcaaaca gaaaaccttt gcaccgcacg cgggcctcct caggcgttga taatttgatt
    23461 aatttcgttg gcgctcaaat ggtactgacg cgggcaggca tgccacacat taagcatgcg
    23521 ctgatatttt tcccacattg gcgtctgggc gttaaaacca tgagtcccag cgtcaaaatg
    23581 ggtatagcgc ccttccacat taaccataaa gcggacataa ccgaggtaac gtgcttcagt
    23641 ggcggcgtca aagccgagga aggtgacacg acgttcatcg atggattgct ggtcctgcaa
    23701 attagaccaa gaaacataca acgcatgata catatccata atgtcgatga tagtgcggca
    23761 ggtttcttct ttcagctcgc caaactcgcg atccaattca cgcatctgta atccgtaacc
    23821 acgctcaata attgtttgca ggcgacggta acgttcagca tttgccggat cgagcatagt
    23881 catcatcttg tactgattag acaaaataag acgttgcgcg ttggtcattt ccattgttga
    23941 ctcctgtatc actctactac ggtgaaaaaa aagaaggctg agtatgcctt cttttatatg
    24001 cgtaatcagg ggtcaattac aaatcatcaa ggaaagtttt atccagttgt ttgaaggcgc
    24061 gcttaagcgt gtcagctaat gcctggtaat caggcttgcc ttcaacgggt gccaacacct
    24121 gtccagactc ctgcaattta ccgcgaactt cataaaacca gttaaggatt gcagggggta
    24181 atggcgttac agaacgcttg cccagccacc acaatccctg catgggtaaa cttaaggcga
    24241 acagtgcagt ggcaactgcc ggcccaagca gaccgcccag ggcaatctgc cagcagagag
    24301 taaatacggc gatcggcggc ataaaacgga tcgcataacg cgtcatcttg ataacgcgat
    24361 tttcgacaaa gaccggagca aggcgttttt ccagtggcca cgtctttgag taatgctgtc
    24421 cgcggcgaaa caagctaaaa aaattaacag aacgattatc cggcgttgac atgcttcacc
    24481 tcaacttcac atataaagat tcaaaaattt gtgcaaattc acaactcagc gggacaacgt
    24541 tcaaaacatt ttgtcttcca tacccactat caggtatcct ttagcagcct gaaggcctaa
    24601 gtagtacata ttcattgagt cgtcaaattc atatacatta tgccattggc tgaaaattac
    24661 gcaaaatggc atagactcaa gatatttctt ccatcatgca aaaaaaattt gcagtgcatg
    24721 atgttaatca taaatgtcgg tgtcatcatg cgctacgctc tatggctccc tgacgttttt
    24781 ttagccacgt atcaattata ggtacttcca tgtcgagtaa gttagtactg gttctgaact
    24841 gcggtagttc ttcactgaaa tttgccatca tcgatgcagt aaatggtgaa gagtaccttt
    24901 ctggtttagc cgaatgtttc cacctgcccg aagcacgtat caaatggaaa atggacggca
    24961 ataaacagga agcggcttta ggtgcaggcg ccgctcacag cgaagcgctc aactttatcg
    25021 ttaatactat tctggcacaa aaaccagaac tgtctgcgca gctgactgct atcggtcacc
    25081 gtatcgtaca cggcggcgaa aagtatacca gctccgtagt gatcgatgag tctgttattc
    25141 agggtatcaa agatgcagct tcttttgcac cgctgcacaa cccggctcac ctgatcggta
    25201 tcgaagaagc tctgaaatct ttcccacagc tgaaagacaa aaacgttgct gtatttgaca
    25261 ccgcgttcca ccagactatg ccggaagagt cttacctcta cgccctgccg tacaacctgt
    25321 acaaagagca cggcatccgt cgttacggcg cgcacggcac cagccacttc tatgtaaccc
    25381 aggaagcggc aaaaatgctg aacaaaccgg tagaagaact gaacatcatc acctgccacc
    25441 tgggcaacgg tggttccgtt tctgctatcc gcaacggtaa atgcgttgac acctctatgg
    25501 gtctgacccc gctggaaggt ctggtcatgg gtacccgttc tggtgatatc gatccggcga
    25561 tcatcttcca cctgcacgac accctgggca tgagcgttga cgcaatcaac aaactgctga
    25621 ccaaagagtc tggcctgctg ggtctgaccg aagtgaccag cgactgccgc tatgttgaag
    25681 acaactacgc gacgaaagaa gacgcgaagc gcgcaatgga cgtttactgc caccgcctgg
    25741 cgaaatacat cggtgcctac actgcgctga tggatggtcg tctggacgct gttgcattca
    25801 ctggtggtat cggtgaaaat gccgcgatgg ttcgtgaact gtctctgggc aaactgggcg
    25861 tgctgggctt tgaagttgat catgaacgca acctggctgc acgtttcggc aaatctggtt
    25921 tcatcaacaa agaaggtacc cgtcctgcgg tggttatccc aaccaacgaa gaactggtta
    25981 tcgcgcaaga cgcgagccgc ctgactgcct gatttcacac cgccagctca gctggcggtg
    26041 ctgttttgta acccgccaaa tcggcggtaa cgaaagagga taaaccgtgt cccgtattat
    26101 tatgctgatc cctaccggaa ccagcgtcgg tctgaccagc gtcagccttg gcgtgatccg
    26161 tgcaatggaa cgcaaaggcg ttcgtctgag cgttttcaaa cctatcgctc agccgcgtac
    26221 cggtggcgat gcgcccgatc agactacgac tatcgtgcgt gcgaactctt ccaccacgac
    26281 ggccgctgaa ccgctgaaaa tgagctacgt tgaaggtctg ctttccagca atcagaaaga
    26341 tgtgctgatg gaagagatcg tcgcaaacta ccacgctaac accaaagacg ctgaagtcgt
    26401 tctggttgaa ggtctggtcc cgacacgtaa gcaccagttt gcccagtctc tgaactacga
    26461 aatcgctaaa acgctgaatg cggaaatcgt cttcgttatg tctcagggca ctgacacccc
    26521 ggaacagctg aaagagcgta tcgaactgac ccgcaacagc ttcggtggtg ccaaaaacac
    26581 caacatcacc ggcgttatcg ttaacaaact gaacgcaccg gttgatgaac agggtcgtac
    26641 tcgcccggat ctgtccgaga ttttcgacga ctcttccaaa gctaaagtaa acaatgttga
    26701 tccggcgaag ctgcaagaat ctagcccgct gccggttctc ggcgctgtgc cgtggagctt
    26761 tgacctgatc gcgactcgtg cgatcgatat ggctcgccac ctgaatgcga ccatcatcaa
    26821 cgaaggcgac atcaatactc gccgcgttaa atccgtcact ttctgcgcac gcagcattcc
    26881 gcacatgctg gagcacttcc gtgccggttc tctgctggtg acttccgcag accgtcctga
    26941 cgtgctggtg gccgcttgcc tggcagccat gaacggcgta gaaatcggtg ccctgctgct
    27001 gactggcggc tacgaaatgg acgcgcgcat ttctaaactg tgcgaacgtg ctttcgctac
    27061 cggcctgccg gtatttatgg tgaacaccaa cacctggcag acctctctga gcctgcagag
    27121 cttcaacctg gaagttccgg ttgacgatca cgaacgtatc gagaaagttc aggaatacgt
    27181 tgctaactac atcaacgctg actggatcga atctctgact gccacttctg agcgcagccg
    27241 tcgtctgtct ccgccagagt tccgttatca gctgactgaa ctggcgcgca aagcgggcaa
    27301 acgtatcgtt ctgccggaag gtgacgaacc gcgtaccgtt aaagcagccg ctatctgtgc
    27361 tgaacgtggt atcgcaactt gcgtactgct gggtaatccg gcagagatca accgtgttgc
    27421 agcgtctcag ggtgtagaac taggtgcagg cattgaaatc gttgatccag aagtggttcg
    27481 cgaaagctat gttggtcgtc tggtcgagct gcgtaagaac aaaggcatga ccgaaaccgt
    27541 tgcccgcgaa cagctggaag acaacgtggt gctcggtacg ctgatgctgg aacaggatga
    27601 agttgatggt ctggtttccg gtgctgttca cactaccgca aacaccatcc gtccgccgct
    27661 gcagctgatc aaaactgcac cgggcagctc cctggtatct tccgtgttct tcatgctgct
    27721 gccggaacag gtttacgttt acggtgactg tgcgatcaac ccggatccga ccgctgaaca
    27781 gctggcagaa atcgcgattc agtccgctga ttccgctgcg gccttcggta tcgaaccgcg
    27841 cgttgctatg ctctcctact ccaccggtac ttctggtgca ggtagcgacg tagaaaaagt
    27901 tcgcgaagca actcgtctgg cgcaggaaaa acgtcctgac ctgatgatcg acggtccgct
    27961 gcagtacgac gctgcggtaa tggctgacgt tgcgaaatcc aaagcgccga actctccggt
    28021 tgcaggtcgc gctaccgtgt tcatcttccc ggatctgaac accggtaaca ccacctacaa
    28081 agcggtacag cgttctgccg acctgatctc catcgggccg atgctgcagg gtatgcgcaa
    28141 accggttaac gacctgtccc gtggcgcact ggttgacgat atcgtctaca ccatcgcgct
    28201 gactgcgatt cagtctgcac agcagcagta atctcgtcat catccacagc tttgcgctgc
    28261 ggatatctga accggaaata atcactattt ccggtttttt attctcttaa tttgcattaa
    28321 tcctttctga ttatcttgct taactgcgct gcatcaatga attgcgccat ctcactttgc
    28381 atacttacca ctttgttttg tgcaagggaa tatttgcgct atgtccgcaa tcactgaatc
    28441 caaaccaaca agaagatggg caatgcccga tacgttggtg attatctttt ttgttgctat
    28501 tttaaccagt cttgccacct gggtagttcc ggtggggatg tttgacagtc aggaagtgca
    28561 gtatcaggtt gatggtcaaa caaaaacacg caaagtcgta gatccacact catttcgcat
    28621 tctgactaac gaagcaggcg aacctgagta tcaccgcgta cagctgttca cgacgggcga
    28681 tgaacgcccg ggcctgatga acttcccgtt tgaagggtta acctcaggat cgaaatacgg
    28741 gacagccgtt ggcatcatca tgtttatgct ggtgattggc ggcgcgtttg gcattgtgat
    28801 gcgtacagga accattgata acggtatcct ggcgcttatt cgccataccc gcgggaatga
    28861 aattctcttt attcctgcgc tgtttattct gttttcactt ggcggcgcgg tatttggtat
    28921 gggagaagag gccgtcgcct ttgccattat catcgcaccg ctaatggtcc ggctgggcta
    28981 tgacagtatt accaccgtcc tggtgactta tattgccacg caaatcggtt ttgccagttc
    29041 gtggatgaac ccgttttgtg tggttgttgc tcaggggatt gccggcgttc cggtgctttc
    29101 tggctccggg ttgcgcatcg tggtgtgggt tatcgccact ctgattggcc tgatctttac
    29161 catggtgtac gcctcacgag tgaaaaagaa tcctcttctg tcacgcgtgc atgagtccga
    29221 ccgcttcttt cgtgaaaagc aggcggatgt tgaacaacgt ccgtttacct ttggtgactg
    29281 gctggtattg attgtcctga cagccgtaat ggtctgggtg atttggggcg tgatcgttaa
    29341 tgcctggttt attccagaaa ttgccagcca gttcttcacc atgggtctgg tgattggcat
    29401 catcggcgtt gttttccgcc ttaacggcat gacggttaat accatggctt catcctttac
    29461 cgaaggggcg cgaatgatga tcgcccctgc cctgctggtg ggtttcgcca aagggatttt
    29521 gctgctggtt ggtaatggtg aagcaggtaa tgccagcgtg ttaaatacta tcctcaacag
    29581 cattgccaat gccattagcg gtctggacaa cgcggtcgcg gcctggttta tgttgctctt
    29641 ccaggctgta tttaatttct tcgtgacgtc cggttctggt caggcggcat taactatgcc
    29701 gttactggca ccgcttggcg atctggtcgg tgttaaccgt caggttaccg tgctggcgtt
    29761 ccagtttggt gatggcttca gccacatcat ttacccaacc tcagcttcgt taatggcaac
    29821 gctcggtgtg tgccgggtgg acttccgtaa ctggctgaag gtgggcgcga ccctgcttgg
    29881 actgctgttt attatgtcca gcgtcgtagt gatcggcgct cagttgatgg gctaccacta
    29941 aaaatgttaa gagccgcaat tgcggctctt ttttcattct gccgtttcag tctctacggc
    30001 ttcatttttg gcattgcgct tcatccacaa cgccagcgct ttcagcgagt ctggagtgaa
    30061 ctcatcgcag cgtgcggtga tttcttccgg cgtcagccag caaactccac tgacttcatc
    30121 ttcctgtagg gcgaagggac cgtgagagac gcaactgaac aacgcgcccc agacacggca
    30181 atttttatct tcgaaataga actgcccgtg ctcggcaaag gggacaccgg caatgcccaa
    30241 ctcttcttcc gcttcgcgac gcgcggattc cagcagttgc tcatcggcct gaactacacc
    30301 gcctgcggtc gcatctaaca tgccgggtaa aaagtctttt gtctcggtac gacgctggac
    30361 cagaattttg cccatgccat catgcacgac gatgtaagtt gcacgatgac gcagacactg
    30421 tgcccgcatt tgttcccggc tggcttgtgc aatgacttcg ttctcttcat tgacaatatc
    30481 cacccattca gtacttgcca aacgacgctg ttccattatc aggaaacctt ctttttctgg
    30541 cgctcttacg gcgcattttg agttgtgggt aaattacgga ttaatcgcga cctgcgcaat
    30601 gatactttga tcattgagtg cgattacact aagtacgtca ttatccagca tgccataacc
    30661 cgccggatta ccgcctttcg gaatactcac cgagccgggg ttgaagtgga aaatctcccc
    30721 ccgctgttct gccactggca gatgggtatg accgtacacc agcacatcgt tctggtttaa
    30781 agcaggtaga ttttccgggc caaaaagatg accatgcgtc aaaaacagac gttgtttttc
    30841 cagtaatacc tgttgccacg gcgcggttat cgggaaatgc agcatcattt gatccacttc
    30901 gctgtcgcag ttgccacgca cagcgataac cttatgtgcc acttcattaa gccgttcagc
    30961 gactttggct ggcgcgtaac cctccggtaa agcattacgc gggccgtgat tcaacacgtc
    31021 gccaaggatc accagccact gggcaccgct ttggacaaac aactccacaa cacgttccgt
    31081 cgccggtaac gacccatgaa tgtccgatgc aaacatcagt ttcatcactc actcctcgtc
    31141 gaaaaaacgt aagctatgat actggattct gtcgttgcta tcagccagat tgcttcgccg
    31201 aaagtgcaat aaaacgctgg acagacgctc gctgccactg gaatgtcgca taatccacca
    31261 ggcgttctgg cacctcatcg ccatgtagta ccaggcggtt aatcattagc gccagatcag
    31321 tatcagcaat gcaccattca ccaaataaat tcggctgacc cagtgctaac aaatgttctg
    31381 ccatcgcgaa cagtttctct gcactggctt ttccctcggc cgttagtggc gcttttttcg
    31441 cccccgcaaa gacaacatcc gtcggacgct cttcgcggat gggcatcaga tcgctgcgca
    31501 gccaggcctg aatctgtcgt gcacgcgcac gattttctaa atcaagcgga taaatacgtt
    31561 cccaggtcgg tggcgcaaat cgatcttcca gatactccgc aatggcagaa gattcactca
    31621 actcaaaatc atcgatttgt aataacggca cacggcgtgt ctgaccgtaa ccttgccacg
    31681 tcggctgcaa atgttcaccg ctgtcgaggt cgatggtctt gatatgaaac gacaggcctt
    31741 tttcctgcaa cgccacccag gcggataaca cataagggga gaaaaagtgg gcatctgacc
    31801 aaagcgtgat agcgggttta ctcattatgt cctcggcaat aaagggggct ggctatcaaa
    31861 ctatagagcc tttttgtcac tgtcacgtga cgaatactca cgtaagtcgc cgcgctttct
    31921 atacttgtgg tgttcagatg attaaacaac cggagctgca atgatcgatc tctatttcgc
    31981 cccgacaccc aatggtcaca aaattacgct gtttctcgaa gaagcagagc ttgattatcg
    32041 cttgattaag gtagacttgg ggaaaggcgg tcagtttcgt ccggaatttt tgcgcatttc
    32101 gcccaacaac aaaattccgg caattgttga tcattctcct gccgatggcg gcgaaccgtt
    32161 aagcctcttt gaggctgctg ccattttgtt gtatctggct gagaaaacag gactcttttt
    32221 gagtcatgaa acgcgtgagc gcgccgccac attacagtgg ttattctggc aggtaggcgg
    32281 actggggccg atgcttgggc aaaatcatca ttttaatcac gcagcccccc aaaccattcc
    32341 ttacgctatt gaacgttatc aggttgaaac tcagcgtctt taccatgtac taaacaagcg
    32401 gctggaaaac tcgccctggc tgggaggcga gaactacagc attgcggata ttgcctgctg
    32461 gccgtgggtt aatgcctgga ctcgcctgcg aattgacctc gcaatgtatc cggcagtcaa
    32521 gaactggcat gagcggatcc gttcgcgccc tgccaccggg caggcactgc taaaagcaca
    32581 actcggtgat gagcgttcgg atagttaaca gaaacaggtt ctcgtgtatt atttcatcct
    32641 aagtaaaaca acggagaacc tgcaatggca caacctgccg ctattattcg tataaagaac
    32701 cttcgtttgc gtacgtttat cggaattaag gaagaagaaa ttaacaaccg tcaggatatt
    32761 gttatcaatg tgacgatcca ctaccccgcc gataaagcgc gcactagcga agatatcaac
    32821 gatgcgctga attatcgcac cgtaacgaaa aacattattc agcatgtaga gaataaccgt
    32881 ttctctttgc tggaaaaatt aactcaggat gtgctcgata tcgcacgtga acatcactgg
    32941 gtgacgtatg ctgaagtgga gatcgataaa ctgcacgcgc tgcgctacgc cgattcggta
    33001 tccatgacct taagctggca gcgttaatcg ccatatcggg aggctgcatg aacatagtga
    33061 tcaccggagg gacgggatta attggtcgcc atttgattcc gcgtttgctg gagctgggcc
    33121 atcaaattac ggtagtgacg cgtaacccgc agaaagccag ttccgttctc ggccctcggg
    33181 tgacactatg gcaagggctt gccgatcaaa gcaacctcaa cggcgttgat gcggtaatca
    33241 acctggccgg agaaccgatt gctgataaac gctggactca caagcaaaaa gagcgtctct
    33301 gccaaagccg ctggaatatc acgcaaaaac tggtcgattt gattaatgct agcgacacgc
    33361 cgccgtcggt actcatttcc ggctcggcaa cgggctatta tggcgactta ggtgaagtgg
    33421 tggttaccga agaggaaccg ccgcataacg aatttactca taaactctgc gcccgctggg
    33481 aagagattgc ctgccgggcg caaagtgaca aaacgcgagt gtgcctgctg cgaaccggcg
    33541 tggtactggc accggacggc ggtattctcg gtaaaatgct gccaccgttt cgtcttggcc
    33601 tgggcgggcc gattggttcc ggtcggcagt atctggcctg gattcatatt gatgatatgg
    33661 tcaacggcat tctctggctg ctggataacg agctgcgcgg gccatttaat atggtttcgc
    33721 cctacccggt acgcaatgaa caatttgccc atgcgctcgg tcatgcgctg catcgcccgg
    33781 ctattttgcg cgtccccaca accgccattc ggctgttaat gggcgaatct tcagtactgg
    33841 tattaggtgg acaacgcgcg ctgcctaaac ggctggaaga agcgggtttt gcgtttcgct
    33901 ggtacgattt agaagaggcg ctggcggatg tcgttcgctg atgtggttta cagcaaacat
    33961 ccgccagtta actccctgtg ttacaggatt agtggctttg cgcgataaga tcgtctggtg
    34021 aaagtcgggt caccatcata actaactctc tgtctaaacc tctatccagc atctcctgag
    34081 caatacgcag ggcttcttcg tgtttgccct gcattgcgcc ttcttcacgt aatctgtcag
    34141 caatggtcat cagtttctcc ttttcttgtg gtgcgcgttc tgttatctca ccaataaatg
    34201 cacggaaacg ctgggcatcc cctgtttgta atacgtaatt aaacagggct tttagctgtc
    34261 tgtcattagt gttccctgta actagcagcg aaacaatttg gtcgactaat cccaacagat
    34321 cgcgctgacg aatatgtttc tgaattaact ccaacagcgc cattttgcgg tgttgcataa
    34381 tctcgtcatc cggcaccacg gtaatatcca ccaacggaaa cgctgatgaa tatattttgc
    34441 gggctatagc aggctcggca aattcatcaa gccagcagag tgaataagga taaggacttc
    34501 tgcaaccatg ataaaacagc atcgggatca ccaatggaag ctctttatag cccgcatcaa
    34561 gatggttttg cattgccgca atggaataac gcatcatgcg aaaagccatt aattcttccg
    34621 gcttactttg gtgctctatc actacataaa tataacccac tccctcctgc gttttcacag
    34681 accacaagag gtcggaataa tattgccgca ggtcttcatc aataaaactg tttggttcta
    34741 gtttaagcgt cgttaaatca cacagtttgc gcagcggcgc gggaagatga atattaataa
    34801 aattccgcgc ggtgtctgga tggcgtaaaa aagatttaaa taccgcatca tgcggcgtgg
    34861 aagttgtcga tattgtcatg gcgttccgtc acccctcaaa tttaagatga cgcgacaata
    34921 accacgatta acacccaatg cagcaggcag tttttatctt tggacagcga attcagaagt
    34981 atttttagcg gactgcaact tttacatttc agcggaagct gactcgcaaa ttccccttga
    35041 aaatactccg gcagcgttag ccaccggaga ggaaattatt tcagcgatcc cttaaggaac
    35101 tgttgcagac gagggctttg cgggttgcca aataactgct ccggcgcgcc ctcttcttct
    35161 attttcccct gatggaggaa aatgacgtga gtagaaacat ggcgagcaaa gcccatttcg
    35221 tgagtcacta ccaccatggt tttcccctct tctgccagtt gctgcataat acgtaacact
    35281 tcgcctacca gttcaggatc gagcgccgag gtaggttcat caaacagtaa aacttctggt
    35341 tccatcgcca gcgcccgcgc gatagaaaca cgctgttgct gaccgccgga aagatgcacc
    35401 ggatatttcc cctgcgcacg ttcgtctatc ccgactttcg ccagatactt caccgcccgc
    35461 tcgcgcgctt cctgcttgct caggcccaac acctgaatcg gcgcttccat gacgttttcc
    35521 aacaccgtca tatggctcca gagattgaag tgctggaata ccatcgtcag gcgtgtgcgc
    35581 agtaagcgca gttgattttt atcggcgact ttgagttgac cgtctttgtc gcgcaccaga
    35641 ttgatcgtct ggccattgac cacgatcgat ccttcactcg gtttttcgag gaagttaatg
    35701 cagcgcagaa aggtactttt ccccgatccc gacgatccga tgatgcttat tacatctccg
    35761 gcattcgctt gcagtgagac ccctttcagc acttcgtgtt cgccatagcg tttgtgcaaa
    35821 tcgataacgt ttaatttatt ctcggacatc gtattctcag tgcgttgaag aaggtttcac
    35881 atgctgcaac cagcgttttt ccgctctgcg aaagaggctg atcaggacat aagagatgat
    35941 taaatagagc accgcggcaa tgccgaaggc ggtaaaaggt tgatacgtgg cggcgttaat
    36001 atcgcgggct attttcagca gatccggcac agtggccgta aacgccagcg cagtagagtg
    36061 cagcatcagg atcacttcgt tgctgtatgc cggtaacgca atacgcagcg cagaaggcaa
    36121 aataatgcag cgatacattt taaaggtcga gaagccatag gctctggcgg cttcaatttc
    36181 cccatgcgga accgaacgga ttgccccagc aaaaatctcg gtagtgtaag cgcaggtgtt
    36241 aagcgtcagc gccagcacgg tacagttcag gccactgcgg aagaaagcgt taaggaattc
    36301 ggttccctta acaatctcaa gcgtgtacat gccggaatag aacaccagca actgaacata
    36361 cagcggcgta ccgcgaaaaa tataggtaaa taaccagatt ggaaactgga tgtatttatt
    36421 actggagaca cgaccaatcg ccagaaacag cgccaggact ccgcctatca ctaccgacaa
    36481 aataagcagc cacagagtga tcgccacacc agtaaagcga taaccgtcgg tccacagcag
    36541 cggtttccag tattcatgta agatttcgat cacaggtcag ccctcttcac acccacggag
    36601 tagcggcgct caaggaacag cagcacacca ttggaaacgg tggtgaaaac caggtaaatc
    36661 acgccacaga cgatggcgaa atagaacggt tcccaggtac ttttgcctgc cagctgcgtg
    36721 gctttgacca catcttccag gccgagtaac gaaaccagtg cggtagattt gaggatcacc
    36781 tgccagttgt tgccaatgcc tggcagggcg taacgcatca tcgccggaaa catgatccgc
    36841 cgaaacactt gcccgcgagt aaaaccgaac gccgtcgccg cctctatatg ccctttcggc
    36901 actgccataa acgcgccacg gaacgtttcg gtaaaataag caccgtagat aaaaccgaga
    36961 gtgataatac cagcgaccat cggatcgata tcaatctgcc cgacgcccat cgcctccgtc
    37021 accatgttta gcgcaatctg caaaccatag aaaatcagca gcatcaacac caagtccggc
    37081 acgcctcgaa tcagcgtggt gtacccttcg aaaatcagcc ccgaaagccg attttgcgag
    37141 agcttaccgc cagcgccaat taaaccgatg attacagcga gcactacaga gctgatagcc
    37201 agctccagcg tgacgagcgc accctgtaaa ataacacctg aaaacccata caacatgctg
    37261 cctgtcctgt cgtgtgtggt gaattaccgc ctcttgtctc cctccggtta cccgaaggga
    37321 gcgggctttc tttcaccgat ggcgattaac caccataaac atcaaaatcg aagtactttt
    37381 tcgctaattt ctcgtaagta ccgtcagcgc gcatttcggc aaaggctttg ttcagtgcct
    37441 cgcgcagttc gttatcttct ttacgcaggc ccatgccggt ccctacgcca aacagttttt
    37501 catctttaac agacggacca ccgaatttgt aatctttacc gacgggttgt ttgaggaaac
    37561 cttcgctggc agcgacctca tcctggaacg cggcatcaat acgtccggca gtcaggtcag
    37621 aataaatgtt gtcctgcccc tgatacgaga cgatttcaat gccttttggt gcccagtgtt
    37681 cattaccgaa cgtctcctgg gtggtgccct gcaatacgcc tacccgtttg cctttcagcg
    37741 actcgactgt cggctgaatg tcagaatttt tcgccaccac cagacgagaa tcggcagcgt
    37801 acagtttgtc ggtgaaggca atttcttgct gacgtttttc cgtaatggaa agcgatgaca
    37861 tgatggcgtc aatcttcttc gcttttaagg acgggattaa cgcatccagc gggttttcga
    37921 caaacgtaca ttgcgtattg atgcgcttgc acaactcctt cgccagctcg atatcgaaac
    37981 caaccagttc gccttgtgaa ttttttgatt caaatggcgc ataggtcggg tcggtaccga
    38041 tgcggatgtt ttgcggaatt gcagcaagtg ccgcagttgc gctggagaag gccagaacca
    38101 gagagagcga tagcaccagt tttttcataa tgtcctcaac tgacagtctt tttataggga
    38161 atatttacag gtttgttaca cttatcgtgc cataaaattg accatcaagg caaaatatcc
    38221 cgccccgtta gacgtattat ttttcattaa aaatgcttaa gtatgcattt caatgcacca
    38281 cgatagtgca acaaacctgg cgtgcaccat gatgatcatt ccatcaggta cagcttccca
    38341 gcgacgtatc agtcaccgta gacattaaag tcgaaatact ttttcgccat cttgtcgtag
    38401 gtgccgtcct gacgcagctc gccaagcgcc ttattgaagg cagccgtcag ttcagcataa
    38461 tctttacgta gccctacacc ggtgccatca ccgaagtatt ttttgtcttt tactgatgag
    38521 ccagcaaaag cgaaatcttt accagcaggt tgcttgagga atccttcgct ggcagcaact
    38581 tcatcttgta acgcagcatc cagacgtcct gcagccagat cggaatagac caaatcctgg
    38641 ttggcatagg ccaccacatc cacgccttta ctacgccagg tctcgttagc gtaagcttcc
    38701 tgggttgatc cctgcagcac accaacatgt ttacctttca gtgaatccag cgttggctga
    38761 atcggtgaac ctttggcagc aatcaaacga gaatctgcgg cgtacagctt gtcggagaag
    38821 gcaatctcct gctgacgttt atcggtaatg gaaagcgacg aaataatagc gtcgattttt
    38881 ttcgctttca gtgaggggat cagcgcgtca aagtcactgg caacccaggt acatttcacc
    38941 tgcatccgtt tgcacatctc gttaccgaga tcgatatcaa agccaacaaa atcaccttta
    39001 gcatctttcg acgagaacag cgcataggtg gtatcggttc cgatacgtac cgtctccggt
    39061 agcgccgcat agctggaagc cgctgcggag agaccgacta ataaagacag agcgagaatc
    39121 gacttcttca tacataaccc tcaagtgaaa atggcttttt tatgttgtgt attgtgttgt
    39181 gtgtttgcag gctctttcat gcaggtctta tgccatcttg acgacaacag taacattcaa
    39241 cgttaaatat gttaataaga cattgcatta ttgtcctgaa gttgaagata gcaggtatag
    39301 cggttgaatc gcagcgtttc gattgaatgg cagaaacaaa atgtcgagga tttgatcgcg
    39361 gttgcaaaat tgccctgaaa cagggcaaca gcggagttat gcgccctgcc agcgggcaaa
    39421 gagatcttca ggaagggtta ttgcaaactg gtcaagaaca cgattaaccg tctgatttat
    39481 cacatcatca agggattgcg ggcgatgata aaacgccgga acgggaggca taatcaccgc
    39541 accgatttct gccgcctgag tcattaaacg cagatggcct aagtgcaatg gtgtttcacg
    39601 cacgcagagc accaacgggc gacgctcttt cagcaccaca tctgccgcac gggtcagtaa
    39661 gccatcagta tagctatgga caatgccgga cagggttttg attgaacagg gtaaaatcac
    39721 catccccagc gtctggaaag aaccggaaga gatgctggcg gaaatatcgc gcgcatcgag
    39781 cgtgacatca gctaatgcct gcacttcgcg cagggaaaaa tccgtttcga gggataaggt
    39841 ctggcgcgcc gcctggctca tcaccagatg cgtttcgata tctgtgacat cgcgcagaac
    39901 ctgtaataag cgcacgccat aaatcgcgcc gctggcaccg ctgatgccta caatgagtcg
    39961 tttcataaat agttgccctg tcagacttgc aggcagactt tgcagtattt cggcgggagt
    40021 tgcaagtcag ggtgccagac cggcaccctc agcgaaggca tcatccttcg ttatgcattt
    40081 cgagattttc cacttcgttc tgacgttgca ctgctttggc gtcatcatta cgtaacgtat
    40141 cgaggaaatc gaggtagccc tgatcaacat ctttggtgac gtagacgccg ttgaacaccg
    40201 agcattcaaa ctgctggata tccggatttt cagcgcgaac ggcgtcgatc agatcgttca
    40261 gatcctggaa aatcaacccg tcagcaccga tgatctggcg aatttcatca acttcgcgac
    40321 cgtgagcgat cagttccgtg gcgctcggca tatcaatacc ataaacgttc gggaagcgaa
    40381 tttccggtgc cgcagaagcg aggtacactt tcttcgctcc ggcttcgcgt gccatctcga
    40441 taatctgctc agaagtggtg ccacggacga tggagtcgtc gaccagcagg acgtttttat
    40501 cgcggaactc ggcgcggttg gcattcagtt tacggcgcac ggacttacga cgcagctgct
    40561 ggcccggcat gataaaggtg cggccaacat agcggttttt aacgaagccc tggcggtacg
    40621 gtttgcccag aatacgagca atttccagcg cgatatcaca cgaggtttct gggatcggga
    40681 tcaccacgtc gatatccaga tcttcccatt cgcgggcaat tttctcgccc agtttcgtgc
    40741 ccatattcac acgcgcgctg taaacggaaa ttttgtcgat aaacgagtcc gggcgggcaa
    40801 agtatacata ctcaaacagg cacggattgc tgaccggatt gtcagcacat tgacgggtaa
    40861 acaactgccc ttcttcagtg atgtaaatcg cttcgcccgg cgcgacgtca cgcaggaaat
    40921 caaagcccag cgtatcgagc gctacgcttt cggaagcgac catatattct gtacggttct
    40981 cgtcaatatc acgttttccc agtaccagcg gacgaatccc gtttggatcg cggaaagcaa
    41041 ccataccgtg gccgataatc atcgccacac aggcatacgc gccgcggatt aagcggtttg
    41101 tggcagcaat ggcagcgaaa atattgtcgg cttccagcgg gtagtggcgg aagttgtcca
    41161 gctcgctggc gaagatatta agcagaattt ccgagtcgga agtggtgttg atgtggcggc
    41221 gtttttcttc aaacagtttt ttacgcaact cgtgagcgtt ggtcagattg ccgttgtggg
    41281 caagcgtaat gccatacggg gagttaacgt aaaacggctg cgcttcagag gcgctggagc
    41341 tgccagccgt agggtaacgc acatgaccaa tgcccatatt gccctgcaaa cgctgcatat
    41401 ggcgagcttc aaatacatcg ctcaccagcc cgttcgcttt acgcaaacgg aagcagttat
    41461 tggcatctat ggtgatgatg ccggcggcat cctgaccgcg atgctgaagc accgttaagg
    41521 catcataaat cgactggtta accggcataa caccggcgat accgacaata ccgcacatac
    41581 gtctttttcc tcgttaagcc acatctcaga gcacttacgc tctgggcaag aaacttgacg
    41641 agctttgcag ataatcaaaa aaccatctga tgataaaact gaattgcggg atcagctgtg
    41701 atttgctcca gtcttcactt ttcgacaccc cggtaaagga gtcgagaaag aagagaatgg
    41761 cggcaacaat caacacaccg cgcaacgcac cgaaacagac gcccagcacc cgatcggtgc
    41821 ctgacaaccc cgttttctcc accaactggc ctatcacgaa gttcacgata gcaccaacga
    41881 tcagggtagc gataaacagt accgcgatgg caatcccatt tcgaaccagt tcgtcttcaa
    41941 agcccgtaaa ccagactgac aggtaagtgt agtaatgact ggcaacaaag aaagcacaac
    42001 cccatgtcac cagcgataac gcttcacgaa caaagccgtg gatcaggcta accagagagg
    42061 aaaaagcaat caccgcgatt atggcgtaat caatccagac catatgtgtc ccacgatttt
    42121 acgccctgtc atcctgttcg gggcgcattc taacagaaaa agaaaacgtt tgcgtaggga
    42181 tttccttccc gcgcatcaat aaaaatggcg ctgaaaaaat attcaacgcc atcgactttt
    42241 tatgcctttg cggcatcgtg caatgcgtgt cggatgcggc gtaaacgcct tatccgacct
    42301 acggttctaa cactgcccaa gcctgataag acgcgccagc gtcgcatcag gcaagactgt
    42361 attaattcgg cgtatagccc attaccacgc cacttaagcc agaaagttgc ttcaactcac
    42421 ccagcgaacc tttcagctta tctttcgagg catccggccc aaccagaata cgggtaattt
    42481 taccctgcac tggcgtggat ggcgacgtat aaacccgata accggcaccg cgcagcttac
    42541 cgacaatctc attcacttta tcggcatttt tcagcgcacc cagttgcaca acataggctt
    42601 tacccgttgg cgctgctttt tcttccacga ccggcttcgg ttctggcttc ggcgcaggtg
    42661 gtacttcaac ctttggttta ggcggctcca ccggtttcgg cttcggtggg gcgaccggtg
    42721 caggttccgg ttcaaactcg gtgttattgg ctgcaatagt ggccggatcg agcgacggtg
    42781 ctgcggcatc acctgcccgc acctcttccg ctgcgccttc cggcggctgc gtcggcaacg
    42841 cctgggtggc ggctggcatc atatcaggct catcacgatc gcccgctttc ggcaccagcg
    42901 ggatagccgc gaactcatcc tgataatgtt ttttctgccc gtccagcagc cctggaagta
    42961 caatcacccc cagcgccacc agcacgatcg tgcccactaa ccgattctga aacttacttg
    43021 ccaccgcttc tcctcgcgtc aatcacttcc atgacatgtg cgaccgtgtg gaaagaacca
    43081 cacaccagca cggtgtcttc cgctttagcg tccgccattg cggcatccca tgcctgcgca
    43141 acgctatcaa atgatttgcc gttacccaaa tgctcaagca gttgttctgc cgtggcaccg
    43201 cgcggccctt ccagtggcgc acaataccag tcatcaacca cgcttttcaa ccaggccaga
    43261 gttccggcaa tatctttatc atgtagcata ccgataaccg ccagcacgcg cccgtttttc
    43321 ggtagcgctt tcatacgccc ggtgagatat tccgccgcat gtggattatg cgcgacatca
    43381 aaaataacgc gtggcgactc gctcacaatc tggaaacgtc ccggcaaaat tgcgctggca
    43441 atcccgtcgc gaatggcatt ttcactgact tccagcccgc tggcacgcag tgccgccagc
    43501 gccgttgcgg cattcggttg cgggacaagc ggcaacggca gattttccag cgtgccgtga
    43561 gcatcgctaa acgcccagtc atgatcggtg acggaatagt tccactcaac gccccgacgt
    43621 tgtaacagtg cacctttttc ctgcgccaca tcagcaatgg tagaaggcat ttccggctca
    43681 ccgacaattg ccggtttttc gctgcggaag atgcctgctt tctcgagacc aatactttcg
    43741 cgatctggac ccagccagtc ggtatgatcc agcgcaatac tggttactac cgcgacatcg
    43801 gcatcgacaa tattggttgc gtccagacga ccgcccagcc ctacttccag aatcaccacg
    43861 tcaagttgtg cctgcttgaa cagccacaac gccgacagcg taccgtactc gaaataggtc
    43921 agggaaatat caccgcgtgc cgattcaatc tccgcaaaag aggcggtgtg ggccgattcc
    43981 ggcaattcct ggccctgcac acgtacgcgc tcggtataac gcaccagatg aggcgaactg
    44041 tagacgccca ctttgtaccc tgccgccatc agaatcgact ccagcgtacg gcaggtggtg
    44101 cctttgccat tcgtacccgc aacggtaaac acaaatggcg ctggtttcag gacgccaaga
    44161 cgcgccgcga cctggctcac gcgctcaagg ccgagatcga tagttttact gtgcaggttt
    44221 tccagataag aaagccacga agccagaggc gacgcggctt gaggagtgcg tttgataatc
    44281 atggtatccg ctgattcgtt acggtgagaa tagcaaaagg gcagagccag tggccctgcc
    44341 cttatcagtt atcaggcctc aggttcctga tccggtaccg ggggtaccac tacgccttca
    44401 cgcggcgctt caggattcgg cgctggcaga ttcatcaact tcgccagaat gctcgccagt
    44461 ttcaggcgca tttccggacg acggacgatc atgtcgatcg cgcctttctc gatcaggaat
    44521 tcactgcgct ggaatctagg cggcagtttt tcgcgaacgg tctgttcgat aacacgcgga
    44581 ccggcaaagc cgattaacgc tttaggttca gcgatgttga gatcgcccag catggcgaaa
    44641 cttgcagaaa caccgcccat cgtcgggtcg gtcagcacgg agatgtacgg caagccgcgc
    44701 tcctgcattt ttgccagtgc cgcagaggtt ttcgccatct gcatcagcga catcagtgct
    44761 tcctgcatac gtgcgccacc agaggcggag aagcagatca gcgggcagtt atcttcctgc
    44821 gcctgctcaa cggcacgcac gaaacgtgca cccacaacag accccattga accgcccata
    44881 aaggcgaact cgaatgccgc agcgacaacc ggcattccat acagagtgcc tttcatcacc
    44941 accagcgcat ctttttcgcc ggtttctttc tgcgcagatg ccagacggtc tttatacttc
    45001 ttggagtcac gaaacttcag ctcatctttc ggctcaagct cgctacccag ctccacaagg
    45061 cttccttcat ctaacaggct atgcaggcga ttacgcgctg tcatacgcat gtgatggtca
    45121 cacttcggac agacctcaag attacgttcc agctcagcgc ggtataaaac ctgaccgcag
    45181 ctatcacact tagtccacac cccttcagga atgctcgcct tgcgggtggg agtaatgttg
    45241 cttttaattc gttcaatcca gctcattagg gacctttctg tctgaacctg gttcgatgcc
    45301 agttttatct ttggggacgc ataatgccat ttttgccccc aacagaccat gaatgttgca
    45361 cattaaaaca taacagcccg aaactttgga taaaaaagtg gtcgaaccgc ggagttactt
    45421 tttattttgc ggcgcgtgcc gcagcgcgtt tgtgacggat tatttcgatg acgcccggca
    45481 aaatagaaac cacaataatc ccgacgatca gcagcttaag gttatcctga accatcggga
    45541 ttgtaccgaa gaaatagcct gcgtaggtaa aaagcagtac ccacaacagt gcgccgatca
    45601 cgttataagc agcgaaatga cggtacgaca tgtggcccat tcccgcaaca aacggggcga
    45661 acgttctgac gatcggcaca aaacgggcga gaataatcgt tttgccgcca tgtttctcat
    45721 aaaactgatg ggttttgtcg agataactgc gacggaaaat tttcgaattg ggattactga
    45781 ataacttttc accgaacaac cgcccgatag tgtaattgac cgcgtcaccc acaatcgcgg
    45841 cgatcagcat cagcactacc atcatatgga cgttgagatc gttggtttcc agtgatgcca
    45901 acgctccggc aacaaacagc aatgaatcac ccggtaaaaa cggcattacc accagaccgg
    45961 tttcacagaa caaaattaag aacaaaatgg cataaaccca gacgccgtac tccgcgacta
    46021 gctctgccag atgcacgtca atgtgcagga taaaatcgat gaggaaataa atcaggtcca
    46081 tatttgccta tgccttgtac tcgtcatctt tcaggctgta actacgttgg ctacagcctg
    46141 aaatgttccg agcattattc tcgttagtcc gccagaaata gcgggcccat tggcggtttt
    46201 ggaagatcat accggtcagg gtaatccacc gcgaccagat acagcccttc cgcttttgcc
    46261 gttgctgccg ccagcgttct gtcctttgcc gccagcaact ctgctatcca gctctccggc
    46321 tggttgtggg caccgacttc catcaggctg ccgacaatat tcctgaccat atgatgtaca
    46381 aaggcattcg ctttgatatc aaccaccaca taaggaccgt gacgcgtgac attaatatgc
    46441 ataacgttgc gccacggggt tcgggactgg cactgcaccg cacggaacga ggtgaaatca
    46501 ttctcgccca gcaagcattg cgcagcccga tgcatccgtt cagcgtccag cggttcgtaa
    46561 aaatgggtta cccctttact cagtaccgcc gggcgcagcc gatgattgta gatgatgtag
    46621 cgataacggc gagccgtggc gctaaatcgg gcatgaaaat catcaggtac agctttaacc
    46681 caacgcacgg cgatgtcacc aggtaaattc gcatttacgc ccaaggtcca cgccgcgtct
    46741 ttgcgctgcg cggttgtttc gaaatgcacc acttgcccgg taccgtgtac ccctgcgtca
    46801 gtacgcccgg cgcagaagac ggtgatgggt tcgttcgcca cctgggagag cgccttttcc
    46861 agcttctcct gcacactgcg gacttcattc tgccgttgcc agccgtaata cttactgccg
    46921 tcgtactcaa tgcccagcgc aattttataa actggcagtt gttgctggtc ggacattagt
    46981 acagatactc ctgcaccagt ttctcggcga tttttactgc catcagcgcg ccgccaaagc
    47041 gaacgttatc ggccaccgac cagaactgga cttgctccgg cataccgtag tcattacgca
    47101 cgcagccaac agaaagatgc ggcgtacccg aagcatcccc tacctgagtc gggaattcgt
    47161 tctcttcaga gagcacaata tcttcgccct gagcaaacgc atcacgcgct tcttctgccg
    47221 ccagcggacg cagtgcttca aagttgacca tctgggcatg accgtagaat accggtgcct
    47281 ggacgacgct tgccgaaatc atcagccctt cgtcctgcag gattttgcgt acttcgtcaa
    47341 cgatacgacg ttcttcacgc acgctacctt cgctatccgg caacaacggc agcatattga
    47401 acgccagttg acgaccgaag aaatcttctt cgtcaatcgg aatgccgttg agcaatttcg
    47461 cactctgccc cgctaacgca tcgaccgctt ttttgccctg ggcggaggct gaaatcaggc
    47521 tggtaacact gatacgcgac aaaccgccct gatcgattaa cggtttcagt gccgccagca
    47581 gctggctggt cagactgtct ggtacggcga tgacattccg gttgcggtaa tcggtcagta
    47641 caaacgggtt tacttccggc accaccagcg gtacgtcggg ttcgagagca aacagtccgc
    47701 tgctgtcgat caccaggcaa cctgagttgg tcgcttcttc aacccaggca gcggtagctt
    47761 ctttgcctgc gacaaaaaat gccagctgcg cctgcgtcca gtcgaattca gcggcatcct
    47821 gcacggtgat tgttttacca ccaaagcgca gttgttcgcc tgcgctttcg ttacgtgcca
    47881 gtgcataaat ttccccaacc gggaactgac gttcagccag cgtttcaagc agggcttcgc
    47941 ccatagcgcc agttgcgccc aggacggcaa tgttccagcc ttcagacatg gtggtttact
    48001 ccagaaatag caaagctccc tgccaatgtt acagcagaga gcatgaagaa gagattaacg
    48061 tgccggatga tgaacggcgt taaaacccag tttacacagc aatgatgccg cactggcgtc
    48121 atcacaaatt acatacagag atgaccattc acggcgctca agatagtttt tgcgcagttt
    48181 atcgaactca cccggtatcc cggcgacttt acgcagcggt gcgtcatcgc ggcgcacatc
    48241 atacaccaaa tgcaccagcc ttttcagcgt cggttgatcg agcagaccat gcagcgtaat
    48301 gcggccaaac tcaggcgcgg gcagcaacgt atccagcgca acgtgttgtt catgcccaat
    48361 aaacttgcta taggcttcaa acacttgcgt cgtgccacgc gctttaccct ccagggtata
    48421 gcctgcgata tgcggcgtgc cgatatccac ttttgtcagc aattccacat taagttccgg
    48481 ctcgccttcc cagacatcca gcaccacgct taacttctgg ccttcattca ggcaggtcag
    48541 cagcgcggta ttatcgacga ctgcgccacg gcaggcgtta atcagaatcg ctccgggctt
    48601 caggctacgg atcagttttt catccgccag atgtagcgtt ttgtacggac catctttaaa
    48661 gagtggcgta tggaaagtca gaatatccgc gcgctggact aactcatcca gcgagcggaa
    48721 atcaccctca tccccacggt cggcgcgagg cggatcgcaa agtaaggttt taatccctaa
    48781 cgcttccagt cgcgcctgca atcgacgtcc aacgttacca acgcccacga tccccaccgt
    48841 acggtcgtgc agtgaaaatc catcgcgttc ggcaagcatc agcagggagg agaaaacata
    48901 ttccaccacc gcaatcgcat tacagccagg tgcagcggaa aaaccaattc ccgcctgctt
    48961 caaccatgct tcatcgacat ggtcggtccc cgcagtggca gttccaacaa atttaatggg
    49021 ttttcctgcc agcaaagatt cattcacttt cgtgaccgaa cgcaccatca gcgcatccgc
    49081 gtctgccagt tgagcgacgg ggattggacg cccgggaacc gcgatcacct cactcaaacg
    49141 gctaaataag tcgcgggcat aaggcatatt ttcatcaaca aggattttca cgtttgtgtt
    49201 acctgtatga gacgagagtt aaccggacaa gtatgccata atctcgcggc caggcatact
    49261 tgcgaagatt tcaggtataa ggatacgtaa tgatacaacc tatttccggc cctcctcctg
    49321 ggcagccacc aggtcaggga gataacctgc cgtctggcgc gggcaatcag cctttatcca
    49381 gtcagcaacg tacttcgctg gaaagcttaa tgacgaaagt gacctcactg acgcaacaac
    49441 aaagagcaga actgtgggcg ggtatcaggc acgatattgg tctgtcggga gattcaccgc
    49501 tgctttcgcg tcacttccct gccgctgagc ataatctggc gcaacgtctg ctggccgcgc
    49561 aaaaaagcca ttctgcccgc cagcttttag cgcaattagg ggagtattta cgtctgggga
    49621 atcatcgtca ggcggtcacg gattatatcc gtcataactt tggtcagacg ccgctgaatc
    49681 agctctcacc ggagcaatta aaaaccattc tcaccctgtt gcaggaaggg aagatggtta
    49741 ttccgcaacc acagcagcgc gaggcgaccg accgtccttt attaccggcg gagcacaatg
    49801 cgctaaaaca gctggtgacc aaacttacgg cggcaacggg ggaacccagc aaacagatct
    49861 ggcaatcgat gctggaactt tccggggtga aagatggcga gttaattcca gcgaaactgt
    49921 ttaaccatct ggtgacctgg ctacaggcgc gtcagacgct aagccagcaa aatacgccga
    49981 cgctggaatc actacagatg gcgctaaaac aacctttaga tgccagtgaa ctggcggcgt
    50041 tatcggcata tatccagcaa aaatatggcc tttctgcgca atcatcgctt tcttctgccc
    50101 aggccgagga tattcttaat cagctttatc aacggcgggt taaagggatt gatccgcgtg
    50161 atatgcaacc gctgcttaat ccttttccac cgatgatgga cacgttgcaa aatatggcaa
    50221 cgcgtcccgc gctgtggata ctgttagtcg cgattatcct gatgctggtc tggctggttc
    50281 gttaaccccg acgaaatgac agtatcgtga caataatacc cagcaccgca gagatcgccc
    50341 cggcaagaaa taccgaagag taaccaaacg tggtcgccag cattcccgcc agcggcccgg
    50401 agacgccgag ggcgatatcc tgaaacgcgg cgtaaccgcc cagtgcggtg ccgcgaactt
    50461 gtgaggggac gcgtttaacc acctccacgc ccagcgcagg aaagataagc gaacatccgg
    50521 ctccggttaa cgccgcgccc gctaatgcga cccatgcacc tggggcttgc cagagcagca
    50581 acaagcccac cgtttctaca agcagagaga caatcgccac tttcacgccg ccaaaacggt
    50641 ccggcatcca gccaaacatg acgcgcatca cgacaaatgc gccgccaaac gcggtaagag
    50701 taaagcccgc catcgcccat cctttgctgg caaagtagag cgaaacgaaa gtcccgataa
    50761 ccgcaaaacc aacgccttgt agtgccagcc ctaaccctgg tttccagata agcccgacaa
    50821 cgctccacag cgatggacgt tctcccgcca gggccggtac tttgcgcact gtgccgttac
    50881 aggcccacgc cagtaagggt aataccattg tggtgatcgc cagtgcggca aaaccgtaat
    50941 ggctatgaat caacaggcca agcggcgcac caacagcgag ggcaccgtaa atcgccattc
    51001 cgttccatga catcactttg ccagagtgtt ttggccctac gatgcctaac ccccaggtca
    51061 gagcgcctgt cagtaactgg ctttcaccaa acccaagaat caaacgcccg atgaccaaca
    51121 gggcaaattt gaacggtgcg gagacaggca aaatcgccgc cagcagcaac gcgccgccag
    51181 ccagaccaca agctaacatc ccctgaagcg ccgaacgttt tgcgccatat tgatcggcca
    51241 gtcgcccggc gtaaccacgc gtcagcaccg tagccagaaa ctgaatcccg acggcaatgc
    51301 cgaccatggt attgccatag cccagatcat gatgaacaaa cagcgggata accggcaacg
    51361 gcaaccctac ggtcatgtag gtgagaaaaa ccgcaaaaga gatgcggaag agcgaaaaac
    51421 tggcagaaga tcgtgtttcg gtttggctta cagcagtcat gcattactcc agaatgcagc
    51481 gcaaggcgag gagtatcccc gtctcatctc tctggtttca gggttacggt gcgttggcag
    51541 gatttaacgc gtacgtcttt tcagaaggaa atcgacaaag cgggaagttt gcctagaact
    51601 ggtggcgatt gtcaatgatg tgaaaaaggg aaccatcagg ttcccttttg cgttagtgcc
    51661 ggaggccgca ttggcgcgta acgtcggatg cgacgctggc gcgtcttatc cgacctactc
    51721 cggcctactg cgaattaatc ttttcagctt gcgcattacc agcgtggcgt tggtgccgcc
    51781 gaagccgaag ctgttagaca taacagtggt cagttcgcga tcggtcgttt cggtcacgat
    51841 gttcagaccc gcagcctgct cgtccagctc ttcaatgttg atgctcgggg cgataaagcc
    51901 gtgttccagc atcagcagag agtagatagc ttcctgcaca cccgcagcgc ccagtgagtg
    51961 accggtcatg gctttggttg cagaaatcgc cgggctctta tcgccgaaca cttcacggat
    52021 agctgccagc tctttcacgt cgccaaccgg agtcgaagta ccgtgagagt tcaggtaatc
    52081 gattggggta tcaacgccat gcatcgccat ctgcatacaa cgtactgcgc cttcgccaga
    52141 cggagcaacc atgtctgcac catcagaggt tgcgccgtag ccaacgattt cagcatagat
    52201 gtaagcacca cgcgccagcg cgtgttccag ctcttcaacc actaccatac cgccgccgcc
    52261 agcgataacg aaaccgtcac ggtgagcgtc gtaagtacgg gaggcttttt ccggggtgtc
    52321 gttgtattta gtagatagcg cacccatcgc gtcgaattcg caggccattt cccagcacag
    52381 ctcttcgccg ccgccagcaa acacgatgtc ctgtttgccc agttggatct gctctactgc
    52441 gttaccgata cagtgtgcgg aagtcgcaca cgcggagctg atggagtagt taacgccatg
    52501 aattttaaac ggcgtggcga gacaggcaga aacgccggat gccatcgctt tagtgaccac
    52561 atacgggcca accgctttca ggccgcgcgg gccgcgcatt gcgtcagcgc cgaacacctg
    52621 gaaacgcggg gaaccgccgc cggaacctgc aatcaggcca acgcgcgggt tattctggta
    52681 agcttccgga gagaggcccg catcagcgat tgcctgctcc atagaaagga atgcataaat
    52741 ggatgcgtcg ctcataaagc gcacaacttt gcggtcaatg aggccagtgg tatccagttt
    52801 tatgttgccc cagacgtggc tacgcatgcc ggaatccttc agctcctgag agaaagtgat
    52861 ccctgaacgt ccttcacgca gagatgccag gacttcctgc tggttattac cgatgctgga
    52921 aacaatgccc aggccagtaa tcactgcacg tttcattcaa tacctctgta agtcgcacat
    52981 agagtaagtt tcgaatgcac aatagcgtac acttgtacgc cgaacaagtc cgatcagcca
    53041 tttaatagag atatttgcgc agccttacac acatcgctaa gatcgagcca ccgcctgtaa
    53101 gacgagtaac ttacgtgaaa cactactcca tacaacctgc caacctcgaa tttaatgctg
    53161 agggtacacc tgtttcccga gattttgacg atgtctattt ttccaacgat aacgggctgg
    53221 aagagacgcg ttatgttttt ctgggaggta accaattaga ggtacgcttt cctgagcatc
    53281 cacatcctct gtttgtgata gcagagagcg gcttcggcac cggattaaac ttcctgacgc
    53341 tatggaaggc atttgatcag tttcgcgaag cgcatccgca agcgcaatta caacgcttac
    53401 atttcattag ttttgagaaa tttcccctca cccgtgcgga tttagcctta gcgcatcaac
    53461 actggccgga actggctccg tgggcagaac aacttcaggc gcagtggcca atgcccttgc
    53521 ccggttgcca tcgtttattg ctcgatgaaa gccgcgtgac gctggattta tggtttggcg
    53581 atattaacga actgaccagc caactggacg attcgctaaa tcaaaaagta gatgcctggt
    53641 ttctggacgg ctttgcgcca gctaaaaacc cggatatgtg gacgcaaaat ctgtttaacg
    53701 ccatggcaag gttggcgcgt ccaggcggca cgctggcgac atttacgtct gccggttttg
    53761 tccgccgcgg tttgcaggaa gccggattca cgatgcaaaa acgtaagggc tttgggcgca
    53821 aacgggaaat gctttgtggg gtgatggaac agacattacc actcccctgc tccacgccgt
    53881 ggtttaatcg tacgggcagc agcaaacggg aagtagcgat tatcggcggt ggtattgcca
    53941 gcgcgttgtt gtcgctggcg ttattacggc gcggctggca ggtagcgctt tattgcgcgg
    54001 atgaagcccc cgcgctgggt gcttccggca atcgccaggg ggcgctgtat ccgttattaa
    54061 gcaaacacga tgaggcgcta aaccgctttt tctctaatgc gtttactttt gctcgtcggt
    54121 tttacgacca attacccgtt aaatttgatc atgactggtg cggcgtcacg cagttaggct
    54181 gggatgagaa aagccagcat aaaatcgcac agatgttgtc aatggattta cccgcagaac
    54241 tggctgtagc cgttgaggca aatgcggttg aacaaattac gggcgttgcg acaaattgca
    54301 gcggcattac ttatccgcaa ggtggttggc tgtgcccagc agaactgacc cgtaatgtgc
    54361 tggaactggc gcaacagcag ggtttgcaga tttattatca atatcagtta cagaatttat
    54421 cccgtaagga tgactgttgg ttgttgaatt ttgcaggaga tcagcaagca acacacagcg
    54481 tagtggtact ggcgaacggg catcaaatca gccgtttcag ccaaacgtcg actctcccgg
    54541 tgtattcggt tgccgggcag gtcagccata ttccgacaac gccggaactg gcagagctga
    54601 agcaggtgct gtgctatgac ggttatctca cgccacaaaa tccggcgaat caacatcatt
    54661 gtattggtgc cagttatcat cgcggcagcg aagatacggc gtacagtgag gacgatcagc
    54721 agcagaatcg ccagcggttg attgattgtt tcccgcaggc acagtgggca aaagaggttg
    54781 atgtcagtga taaagaggcg cgctgcggtg tgcgttgtgc cacccgcgat catctgccaa
    54841 tggtaggcaa tgttcccgat tatgaggcaa cactcgtgga atatgcgtcg ttggcggagc
    54901 aaaaagatga ggcggtaagc gcgccggttt ttgacgatct ctttatgttt gcggctttag
    54961 gttctcgcgg tttgtgttct gccccgctgt gtgccgagat tctggcggcg cagatgagcg
    55021 acgaaccgat tccgatggat gccagtacgc tggcggcgtt aaacccgaat cggttatggg
    55081 tgcggaaatt gttgaagggt aaagcggtta aggcggggta atctgctctc gcagtgtttg
    55141 ccggatgcgg cgtgaacgcc ttatccaacc tacgtgtgac gtgtaggcct gataagacgc
    55201 gataagcgtc gcatcaggca tggtgctcca gacgccgcaa cttacagctg tgacgcctgc
    55261 tgaaacaggt tttcccacat atcggtcacc agcgcctggt cacgcggcga caattcaccg
    55321 gcaccaatgg ctttttccag actctggcta acggtcgtat gcaccgcctg agcggagtgg
    55381 tcatcaccac tttccagttc tgcgatggct aatgtcaggt ggccacgcaa atacccactg
    55441 gcaaacaact catcatcact ggcatggtca accatgccgt cgattaatgc cagaatgcgt
    55501 gattcaaact ccgcgatcat cttctttcct cattgtaaaa cgtggcgctg gcttcagttg
    55561 agcgcttccg gccacgggaa ctgttccgcc gtaagttccg gcgtgtgata ataattttgt
    55621 agtgctttaa tgaagcgtgc cgggcgttcc ggaatgccat ttgtcagata atccatcacc
    55681 tgcgcatgta cccgccgttg aaacaccaca cgatccggtt cgaaatcccc ttccagattg
    55741 tcgcaactga cgttaaacgg atatccagcc gccacgcaga ataaccagtc gaacgcctgc
    55801 ggcttcactt caacatcttc aaactggctt tgcgtctggg catcgcgtcc gtccgggcaa
    55861 taccagtagc cgaaatcaac cagctcacgg cgcgcttttc cggcaataca ccagtgcgaa
    55921 atctcatgaa ttgcgctggc ataaaagcca tgagcaaaga cgattcggtt atacggtact
    55981 tccgcatcag caggaagata gatcggttcg tcgtcgcctt taatcagacg ggtattaaat
    56041 tcatcggcaa agcagctatt aaaaatttca atcaactgct cgtagtggtg tgtactgttc
    56101 attagttcat ccccaaccag tggaggatct cctgtccgtg gctatcgtaa agtaatttgg
    56161 cactcatcac cgccgagacg ataacaatca tcgggcggat cagcttttgt cctttgctca
    56221 acaccaaccg tgaacccatg cgcgcgccga ggaactgccc caccagcatg acaaagcccg
    56281 tcgcccaaat cactttgccg ccgagaataa acagcagcag accgccaatg tttgacgttg
    56341 cgttgagtaa tttggcgtga gccgtggctt tggcgaggtt gaatccgcac agcataacga
    56401 aggccagcgc gtaaaacgat ccggcagccg ggccaaagaa tccgtcataa aaaccgacgc
    56461 agccaccggc aattaacgcg aagggtaaac cgtacatccg gcgctggcga tcttcttcac
    56521 ccagctttgg catcagcaag aaatagaggc cgatacaaat caccagaatg ggcaaaatct
    56581 gccgtaagac atcagcctga acgtactgta ccaacagtgc gccgctcatt gagccgacaa
    56641 aggtcatggc aatattgagt ttctgatcgc ttaaactaac cactttgcgg cgaataaagt
    56701 agatagtggc ggaaatagag ccgccgcagg cttgcagttt attggttgcc agcgcgttag
    56761 cgggagacat tcccgcagcc atcaatgccg gaatggtgag taacccaccg ccaccggcaa
    56821 tcgagtcgat aaatcccgcc agcatggcga caaaaaagag aactcccagc aacagcggag
    56881 aaaccataaa caggctatta aacgtttcca ttagatcacg tgctcatcca gtagcgcctg
    56941 gcaggaaggc ggcaacggag gcggtgtctt cttctcaggc tttgttgttc ccggttttgg
    57001 aggttcaaac cagctttgca gttctgcccc gcaaccatcg cctgatggcg gtaaaggttg
    57061 atcttcacac tccagactat cggcagggca acgtaatcgt acatgcatat gcgcgcgatg
    57121 ctggaaccag ggtcgcactt tgcgcaacca gtcgcgatcg gtgcccgcat caaggcaaag
    57181 ttgttgttta atcgccggat taacaaaaat gcgcgtgacg tctttgtcct gggcggcgag
    57241 tttgatcaag ctgaaaattt ctggcttcca cagcgtggag acaacgtgtt taccgtcgcg
    57301 ggaaactaag tccagtgctt gcgggcgcaa gagctgcgcg gaggtccagc gagttttcgg
    57361 cagttgcaga aagatatcga catccagtcc ggtctggtgg ctggcatgac cgccgttgaa
    57421 acgcccacca gcgggcatcc ccatatcgcc aatcagcacc gtacccatgc ccagattgct
    57481 cacctggcta ctcagacgct ggataaacat caccagatcc gggtgaccga aatagcgacg
    57541 ctgatcggta cgcatgacct gataatgttc ggactgtatc ggcaacgtgt cagcgccgac
    57601 aatacagcca ttagaaaaac tgcctatcga ttgtgcgcta cccggcacag gttgggttat
    57661 tttttgccac ggcgtcgctg ccaggctagc actactggca agcagagcca gcagcgcaat
    57721 cgcggttttg ttcatttttt accagcgtgg aatatcagtc ttcacatcgg cattttgcgc
    57781 ccgctgccgt aacaggtgat ccattaaaac gatcgccagc atcgcttcag cgatcggcac
    57841 tgcacggatc ccgacacagg gatcgtgacg gcctttggtg atcatctcaa cttcttcgcc
    57901 aaagcggtta atagtgcgcc ccggcacggt aatgctggag gtcggtttca gcgccatatg
    57961 agcaatgatt tgctgcccgc tgctgatacc gccgaaaatg ccgcccgcat ggttgctctg
    58021 gaaaccgtct ttggtgattt cgtcgcggtt ctggctgccg cgcagcgcca ctacgtcaaa
    58081 accgtcgcca atttccacgc ctttcaccgc gttgatgctc atcagcgcat gggcgatgtc
    58141 ggcatccagg cggtcaaaga ccggctcgcc aagtccggcg gggacaccac tggcaacaac
    58201 ggtgactttc gcgccgatgg agtcgccctc ttttttcagc gcgcgcatca gttcatctaa
    58261 cgcgtcgatt ttgtcggggt ccgggcagaa gaacggattt tgttcaacca gcgaccagtc
    58321 tttgatttcc agcggaatgt cgcccatctg ggtcaggcag ccgcggattt caataccaaa
    58381 tttctcggcg agatattttt tggcaatcgc ccctgccgcc acgcgcatgg cggtttcgcg
    58441 ggcggaagaa cgtccaccgc cgcgataatc gcgcagaccg tatttttgtt cgtaggtgta
    58501 atcagcatgg cctggacgga agacgtcttt aattgcgctg taatcctgag aacgctggtc
    58561 ggtattttcg atcaacaagc caatgctggt gccagtagta acgccttcaa aaacaccgga
    58621 gagtattttg acctgatccg gctcgcggcg ctgggtggta tagcgcgatg tcccagggcg
    58681 acgacgatca aggtcatgtt gcaggtccgc ttccgtcagc ggaatgcctg gcggaacacc
    58741 atcgacgatg cagccgagcg ccagcccgtg cgattcgccg aaggtggtta cgcgaaagag
    58801 ttgtccaatt gtgtttccag ccatcacggc tccgttattg ttgtgtttgc gtgtttactt
    58861 aatctttata aatcgcgaaa tgttctcgtg cggcaataag ctgctcttta gtgagcataa
    58921 acacaccatc gccgccgtta tcaaactcca gccaggtgaa cggaacatcg ggatattgtt
    58981 ccataagatg taccatgctg ttgcccactt cacaaatcaa cacgccatca tcagcaaggt
    59041 aatctgccgc gttaccgaga atgcgacgcg tcagtttcag gccgtcggtg ccagatgcca
    59101 ggcccagttc cggctcgtga cggtattcgt ttggcaggtc ggacatatct tccgcgtcga
    59161 catacggcgg gttagtgaca atcagatcgt attgcacttt cggcaaatca cggaacagat
    59221 cggaacgaat cggaatgacg ttgtggatca gaccgtgttc ttcgatgttc tgttcggcaa
    59281 ccgccagcgc gtcgggagag atatctaccg cgtcgacttc cgcttccggg aaggcataag
    59341 cacaggcaat ggcgatgcag ccgctaccgg tacacatatc taaaatatgc tgcggttgct
    59401 tgctgatgag tccggcaaat ttattgttga tcagttcacc aatcggcgag cgcggcacca
    59461 gcacgcgttc atcgacgtaa aattcatgac cgcagaacca ggctttgttg gtcaggtaag
    59521 ccaccggaat gcgctcgttg acgcggcgga tcacgcgttc aacaatacgg tgtttttcgc
    59581 tggaggtcag acgcgcggtg cgcatatctt ccgggatatc cagcggcagg tagagcgatg
    59641 gtaacaccag ctgtacggct tcatcccacg ggttatcggt gccgtgaccg taccagatat
    59701 ttgccgcgct gaagcggctt accgaccagc gcaatatgtc ctgaatggtt tgcagctcat
    59761 ttactgcttc atcaacgaaa attttatcca cgtattcctc cagggcatga tcgcaataat
    59821 ttcggcggct agtttgccat gaagatgacg ataaatcagc attcacgcgc ggtgagtgag
    59881 gaaaaatacg tttaaaacga tcgattgtgc tacgagtcgg gtaaactgta ggaaaattag
    59941 aaatagagac agataaatga aaaagaaaac aacacttagc gaggaggacc aggctctgtt
    60001 tcgccagttg atggcgggga ctcgcaagat taagcaggac acgattgtcc accgaccgca
    60061 gcgtaaaaaa atcagcgaag tgccggtgaa acgcttgatc caggagcagg ctgatgccag
    60121 ccattatttc tccgatgagt ttcagccgtt attaaatacc gaaggtccgg tgaaatatgt
    60181 tcgcccggat gtcagccatt ttgaggcgaa gaaactgcgc cgcggcgatt attcaccgga
    60241 attgtttttg gatttacacg gtctgacgca gctgcaggcc aagcaggaac tgggcgcgtt
    60301 gattgccgcc tgtcgccgcg aacatgtgtt ttgtgcctgc gtgatgcatg ggcacgggaa
    60361 gcatattttg aagcaacaaa caccgctgtg gctggcacaa catccgcatg tgatggcatt
    60421 tcaccaggca ccgaaagagt acggcggtga tgcggctttg ttggtgttga ttgaagtgga
    60481 agagtggttg ccgccggagt tgccctgaat gctggcagca aatgccggag cagcgtaaac
    60541 gccttatccg gcctacaaat atgtgataat tcaataaatt gcaattcact tcgtaggcct
    60601 gataagacgc atcagagcaa tactacgaac ttcagttcat tcaatacata ctgaaaacga
    60661 cacgtcgaat caatcggttg cccactactg gtattcacat tcaccacaat atcgtgtagt
    60721 actttatccg ttaccggatg acgagttggc gtaaccgtta ccgttgattt atcacaaccg
    60781 taagaggtaa aggttaacaa ttctgatacc gcgcggcagt atgctcctct gccatcatta
    60841 tcagaaaaat tactgccatc acagagtctg ccgccttcca tcaggtaggt cgaaagcgtg
    60901 gtggtcactg ttgaccccgt ggactgaatt cgtaacgcct tattatggct gacataattt
    60961 tcactccagg agatatcgta tgaagcgacc ggtatcgttg ggcatattgt gttatgcact
    61021 gtaggggccg tatagcttcc aaatccgggt aaatctgtat aggaataaaa ggatgcgatc
    61081 caagggcaac cataatcatc agatgcaacg gtaattttaa acgtcatata tgcacaacga
    61141 aaaattttcg tatctatgcc agggcaattt gagttattaa ttgtatcaaa gtcagtcagt
    61201 accaaagttg gagctgaacc cgacccagac caggaaataa cccgcaacgt atcgccttta
    61261 tacgcaaaag aatcatattt cccatatgca acctcctttg gcgttatcac cgtaacattg
    61321 aaaacactgc tcgctacagc accggttacc ttgtccatgg cataataaac tctcacactg
    61381 tcaaaactcg cactcataag tttatctcca gcataactct tagatgcgcc gacaacactc
    61441 agaacgagca gcattaggct cgcgaaaatt atggtccatt ttttcatttc agaaatactc
    61501 aaaggttgcc gtcactgtgg cactaaagtc tccggacgtt acatctcgcc cgctctttgc
    61561 ctgtaaccag gtattaaaat ttaggctgtt atttccctga ttcagcggga gcgtgacgcc
    61621 tgttgtattg ttgattggca ccgatgtgcc tgccgcagtt tcaataccaa tcccagcgcc
    61681 ggatgctgat gacgaggagt caaaggccag aaagccgggt agagcactat cttctgttcc
    61741 tgtcaacgtt actttgacat taaacaatgt agaactatga caatctttca attgaaacac
    61801 caatggcact cgctctgact gcccaaggtt cattaaatca ctggcggcaa tggcaggaaa
    61861 atgtacttct gccaactggc taccattaat cactggcgta cacgatttac tgattaaatt
    61921 gccggtaaag tgcaaattat ctcctgctat tgtggttcca ctgcccagca acatcaggct
    61981 actaataaaa aacaacgtac gctttttctt cattggtaat ctacctgtaa cgtggcccag
    62041 gcctcaaagt ccgcctctgg cagaatgaca ccactctttt ttactggcac agccgttaac
    62101 accggtaaat cggtttcatt caccacaagt ggcgtattga tcgtaaaact ctgccctgcc
    62161 tgtttgagct gaatgcctaa accagtgata ttgctgctta ctgcagcatt atcaaaatcg
    62221 cttggtgtgc cactccagct caaggtcatt tccattaatg ggtcgcgaac cgccgtatcg
    62281 caggtaatgg aatacggcac ggtttgctca taattgctgc cattaatgtc atcaataagc
    62341 acatcctgaa aagttatttc aatggtttga gcgttgctaa tcgtgcagtt aggcggctgc
    62401 actaaataac cgtgaaaaga gacgtttgag agtgccatcg agcaaaagcc ccacagcaaa
    62461 ataagcgaga tccgtttcat tggtaatcca ccacaattgt ggcactggcg ttaaattcag
    62521 ctgccgctaa cgttgtacca ctctgtttca ccggcacagc ttccagaacg ggaactcccg
    62581 aggaaaaatt aaatggcaac catgagccag aggttaagtc gagtattgtg tgatcactac
    62641 ttttctgaac ccgaatacca aacccaggaa tacccgtgct gataactgtt tcaccgttaa
    62701 tcacgaccgt tgtcgcctgc atctgtagac gcaggtcatc cattgccaga ttgttacaaa
    62761 caagcgagta gtcaatggga cgtttataat caaccctatt gatcttatta atgaatacat
    62821 taccaaactc aactgccgca ccatttaccg tacaaggagg aggggcatcg acaagcaccc
    62881 gtaaattcat acttactgac ttactcgctg caactgcaaa gccgctacca acacaacata
    62941 tcagtaaccc agttaccgcc cagacagaac aagatccttt catactggct ctcccggcct
    63001 cagcctttgc ggctctgctc ttcatcgacc tagcaactgt taccgttgca agtgaaaaag
    63061 agcggcatcc gagcgccata gtcgttgaca taggtcagta ccggcacgct acccatcttc
    63121 acattcagcg gtgcagaggt tttcggcgac atcactattg gcgagaaacc ggctgccgga
    63181 ttgccatttt tctgtgtgct ggcattactg aaaatgacgt aatacgacgt cgggttattg
    63241 acggtaaatt cctgaccgtt gcgggttaaa gtaatcttat attgctaggg gtttttcatg
    63301 ctgacgttct ccagcgcctt cggtcgccag aacaacttaa tacgcgtttg tagtgctatt
    63361 tgcagcgtgt tggctttgtt tgattttggc gggatctcac gcacgttgaa gtagaacagg
    63421 ctttcacgat cggcaggcaa tttgttgata tccggcattc cctgcacttt tacctgcccg
    63481 ttcatcattg aatcgatgcg ttgcaccggt ggcagaacag ttaacggtga actgatcttg
    63541 ttaccctttt catcttcgat ccagctttgg gccagataag gcagtttcgg atcgttattg
    63601 cgcaacgtca cgctgattga tttgtcgctt tcattaaata tcaaacgggt acgatctggc
    63661 gttacagaag cctgggcaga caaactggca gcgctaagca gtaacgctag cgtcgtagca
    63721 cccaatttgg cagagcaaag taaatcagac atggatcatt ccttaatcac gttattggtt
    63781 agctgaaaca gaaacagggg gattggtggg tgtcacctgt tgcgtctgct cctgaatgac
    63841 aggctttatg tcatcaggaa caaccggcgc gacgttgcct ttatgctggc atggcagtaa
    63901 caagccgtta aacagatcgg caggcagcgg gtccggcagg ttgatatcgc aatgcgcaac
    63961 accactccag aacacactca tgtgttcgcc aggtttcact ccagccagat aaacattgcc
    64021 gtcatcatcg acaaggccca ctgtctgctc gttatcattt tttacttctg cgccaaacgg
    64081 aggatggctg ccgtcttgta aacgcagcac cgccatcgct ttttgaccac tgatgacggc
    64141 aaatttgcgg tagccaatcg ccccttcagt tagcgtggct tgtaccaccg actgggttgc
    64201 ttcggcgttt tccggcagtt tgttgaggtc gatatacgcc tgattgcggt aatagttatt
    64261 gacgtcagaa acgacggctt taccaaacat attggtataa acagcagcac cgttaccttc
    64321 taccggaaca tcggcaacgc catcggcatc aatcaacaag cgtgtcccgc ccatattctg
    64381 ggtacggtga agtgcgccac cgtgggcagt aagcgttgcg ccgccctgta acgacaagcc
    64441 cgcagaggtg tattgccctt catggtagtt cgcactgagg tcaacctgcg ccagcgaacc
    64501 atcatggctg taatagccgt caacgctggt gtgtttgtca ctggtgccga cgttcaactg
    64561 atagtgagtc gcgtcatcga cacggctgaa ataaccgacc tgactgctgt ccgtcccact
    64621 gccatagtac cgttatagct aacggtactg ttgtcgcccc acggcatact gagcgaaatg
    64681 tacatgcctt tgtcggcctg gttgtcatac tcgtaacggt agccagtcat cgagatgctg
    64741 acattacgaa tactgcccat attgaaatag tgcgagagca tgatgttgta gtttgtctgc
    64801 tcctcgcgat cccagtaggt atggcgggta tagttgagat aaaccgaaac acccgcatcg
    64861 cggaagttct ggttataagt ggcggtgtac atctctttgt cgttgcccgt gcgtaccatt
    64921 ccgctgtcac tggcatccag atactcgctc atggtcataa agttctcttc cgagaagcga
    64981 tatccagcaa aagtaacgcg gctgttgagc tggtcaaagt ctttggaata actcacacgg
    65041 aaggagttac cgtccagcga acctttgcca taagcggtgt ctttatccag tttggtgtgc
    65101 gagtgagtaa catcaaacgc gaccgcgccg aatgtagaca aatcgcgacc gacaccaagc
    65161 gccgcagact gatagttttc atctcccagt gcgccgccat ataacgacca gccgttagcg
    65221 atcccccagg aagcttcagc accagaaaaa aattcaccct cgacatggtg tccccactct
    65281 tgcggacggc ccatcatgat cttatagcga acctgacctg gacgagtgag gtatggcatc
    65341 gaggcggtgc tgatgtcata ttcctgcacc tggccgttct gttcttcaat gcgaatatgc
    65401 aacgtaccgg agacagaatc accaagatcc tgaatacgaa acggtccagc cggaacctgc
    65461 gtttcgtaaa tcacacgccc catctggctg acggtcactt ttgctgtggt gtgtgccacg
    65521 ccggaaatgt ctggcgcgta gccgcgcaga ttgggaggca acatttgatc gtcagtactg
    65581 acactgccac caacatagtt aaaaccatca aaaatatcgg atctgaggta atcctcgccc
    65641 agcgccagtt tggcttttaa tgatggtaac gcccgcctgg catagtagcg actccactcc
    65701 catttttttg agtttcatcg ccgctgaatt catcgtcatc attactgcga gtatgttgat
    65761 agttagtctg ccagtcagca cgcatacgcc acggccctag gttaacccca accgtcccgt
    65821 tgccgctgat ctcgttacta tcatcaccgc cattttcttc gtgccgggtt tgtgcgttga
    65881 tgctgtagtc cgcgacgatc ccggagatgc cgtcatccca acgtgaaggc ggatccctgt
    65941 cgggataagt atattcgagg taagcctgtg gcagtgaaat gactaatgcg gactggctta
    66001 aatcagcctt aatttccatg ccttccagtt gaccggattt caggcattta gcatcgtggc
    66061 tccattgcag atttttcgcc acgtcttctt ttaaaccaaa ctgcgctacc agttccggtg
    66121 tcagacaagc atagcttttg ctcgcgtcat cttcaccagc ataccagtaa atatcgtact
    66181 cttccgccaa tggctgttta tttagttgaa cctgtaaatt gtattttccg ggctcaacat
    66241 atccctggct ggaaaaacgc ttcatatcaa tttttgtgtc gccttttaat tccagaaaac
    66301 gggaatcaaa ctgaatgtcg tcttcagccc agacactgct ataactccct gacattgcca
    66361 gcgcaatgca ccacggaaga atccgtaatc gaaaaagaga atggtcaggc atacacgttt
    66421 ctatccgttt ttattcttgt tcggtcaaaa tacatttaaa tcccccatag cagtgtcgct
    66481 atgggggtat gccttacagg taagtaatct ggaaggtggt atttgcttca aaattaccca
    66541 gatctggcgc atcagctgcg ccgaccagcc aggcgttaaa gttcagtgtc tgcttcgctt
    66601 tgcctttgtt ggtagaagtg tctttcacta ttttctggtc aatacccatg ccgcttttgt
    66661 aagaggtacc cagagagtca ccgatcgcca ggctaacatt gttaaatgcc gcaccggtat
    66721 cggtattgaa aatggtgtaa taattgccgc tatttgcaga agaaacggta ccagtaaagg
    66781 tagtggtcat tgtttcctga gtatcaaata cgcaatcttg cagacgaatc tggaaacttt
    66841 tcggcgtggt ggtgccgtta tttttcagtt tttcggcacc gatatcaccc atatcaactt
    66901 ccagtttgtg atcgtccgga acgatagaac acactgagtc ttcaatggtg ccgtaaaaac
    66961 gtgcagtacc gttgttaccc gcagcgattg ttgccgtaga ggtgaacacg cccatcacca
    67021 ttgccgcagc aatagctgtt ttaacaaact tactcattat ttgtccttaa actataaata
    67081 attaaaaata taaacattgc aatacattga ttcagtcaat agccaatgtt tatacccagc
    67141 cattgaatac ggcatgcgac ccaaaaagaa caggtcgtag aaaattgtat tcattggtgt
    67201 ggggccgcat agtgatgttt ccacacccct gatccaattt aattttacta aagagtaaat
    67261 gtagatttaa ttaatatatt gacagaagaa aggttatctt aagga
//
LOCUS       DAJTRC010000008        67023 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.008, whole genome shotgun sequence.
ACCESSION   DAJTRC010000008 DAJTRC010000000
VERSION     DAJTRC010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 67023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 67023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..67023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.008"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO357_000582"
     CDS             complement(65..1069)
                     /gene="cra"
                     /locus_tag="OO357_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite repressor/activator"
                     /protein_id="HCR5885637.1"
                     /translation="MKLDEIARLAGVSRTTASYVINGKAKQYRVSDKTVEKVMAVVRE
                     HNYHPNAVAAGLRAGRTRSIGLVIPDLENTSYTRIANYLERQARQRGYQLLIACSEDQ
                     PDNEMRCIEHLLQRQVDAIIVSTSLPPEHPFYQRWANDPFPIVALDRALDREHFTSVV
                     GADQDDAEMLAEELRKFPAETVLYLGALPELSVSFLREQGFRTAWKDDPREVHFLYAN
                     SYEREAAAQLFEKWLETHPMPQALFTTSFALLQGVMDVTLRRDGKLPSDLAIATFGDN
                     ELLDFLQCPVLAVAQRHRDVAERVLEIVLASLDEPRKPKPGLTRIKRNLYRRGVLSRS
                     "
     gene            complement(1151..1237)
                     /locus_tag="OO357_000583"
     CDS             complement(1151..1237)
                     /locus_tag="OO357_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fruR/shl operon leader peptide"
                     /protein_id="HCR5885638.1"
                     /translation="MRNLQPNMSRWAFFAKSVGTWNKSSCRS"
     gene            complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OO357_000584"
     CDS             complement(1249..1740)
                     /gene="ilvN"
                     /locus_tag="OO357_000584"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001563086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5885639.1"
                     /translation="MRRILSVLLENESGALSRVIGLFSQRGYNIESLTVAPTDDPTLS
                     RMTIQTVGDEKVLEQIEKQLHKLVDVLRVSELGQGAHVEREIMLVKIQASGYGRDEVK
                     RNTEIFRGQIIDVTPSLYTVQLAGTSDKLDAFLASIRDVAKIVEVARSGVVGLSRGDK
                     IMR"
     gene            complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OO357_000585"
     CDS             complement(1743..3467)
                     /gene="ilvI"
                     /locus_tag="OO357_000585"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020079613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 3 large subunit"
                     /protein_id="HCR5885640.1"
                     /translation="MEMLSGAEMVVRSLIDQGVKQVFGYPGGAVLDIYDALHTVGGID
                     HVLVRHEQAAVHMADGLARATGEVGVVLVTSGPRATNAITGIATAYMDSIPLVVLSGQ
                     VATSLIGYDAFQECDMVGISRPVVKHSFLVKQTEDIPQVLKKAFWLAASGRPGPVVVD
                     LPKDILNPANKLPYVCPESVSMRSYNPTTTGHKGQIKRALQTLVAAKKPVVYVGGGAI
                     MAGCHQQLKETVEALNLPVVSSLMGMGAFPATHRQALGMLGMHGTYEANMTMHNADVI
                     FAVGVRFDDRTTNNLAKYCPNATVLHIDIDPTSISKTVTADIPIVGDARQVLEQMLEL
                     LSQESAHQPLDEIRDWWQQIEQWRALQCLKYDTHSEKIKPQAVIETLWRLTNGDAYVT
                     SDVGQHQMFAALYYPFDKPRRWINSGGLGTMGFGLPAALGVKMALPEETVVCVTGDGS
                     IQMNIQELSTALQYELPVLVVNLNNRYLGMVKQWQDMIYSGRHSQSYMQSLPDFVRLA
                     EAYGHVGIQISHPHELESKLSEALEQVRNNRLVFVDVTVDGSEHVYPMQIRGGGMDEM
                     WLSKTERT"
     gene            3586..3630
                     /locus_tag="OO357_000586"
     CDS             3586..3630
                     /locus_tag="OO357_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885641.1"
                     /translation="MKRNDKILHYEGLN"
     gene            complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OO357_000587"
     CDS             complement(3785..4729)
                     /gene="leuO"
                     /locus_tag="OO357_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LeuO"
                     /protein_id="HCR5885642.1"
                     /translation="MPEVQTDHSETAELSKPQLRMVDLNLLTVFDAVMQEQNITRAAH
                     VLGMSQPAVSNAVARLKVMFNDELFVRYGRGIQPTARAFQLFGSVRQALQLVQNELPG
                     SGFEPASSERVFHLCVCSPLDSILTSQIYNHIEQIAPNIHVMFKSSLNQNTEHQLRYQ
                     ETEFVISYEDFHRPEFTSVPLFKDEMVLVASKNHPTIKGPLLKHDVYNEQHAAVSLDR
                     FASFSQPWYDTVDKQASIAYQGMAMMSVLSVVSQTHLVAIAPRWLAEEFAESLELQVL
                     PLPLKQNSRTCYLSWHEAAGRDKGHQWMEEQLVSICKR"
     gene            5388..5474
                     /gene="leuL"
                     /locus_tag="OO357_000588"
     CDS             5388..5474
                     /gene="leuL"
                     /locus_tag="OO357_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leu operon leader peptide"
                     /protein_id="HCR5885643.1"
                     /translation="MTHIVRFIGLLLLNASSLRGRRVSGIQH"
     gene            5567..7138
                     /gene="leuA"
                     /locus_tag="OO357_000589"
     CDS             5567..7138
                     /gene="leuA"
                     /locus_tag="OO357_000589"
                     /EC_number="2.3.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-isopropylmalate synthase"
                     /protein_id="HCR5885644.1"
                     /translation="MSQQVIIFDTTLRDGEQALQASLSVKEKLQIALALERMGVDVME
                     VGFPVSSPGDFESVQTIARQVKNSRVCALARCVEKDIDVAAESLKVAEAFRIHTFIAT
                     SPMHIATKLRSTLDEVIERAIYMVKRARNYTDDVEFSCEDAGRTPIADLARVVEAAIN
                     AGATTINIPDTVGYTMPFEFAGIISGLYERVPNIDKAIISVHTHDDLGLAVGNSLAAV
                     HAGARQVEGAMNGIGERAGNCSLEEVIMAIKVRKDILNVHTAINHQEIWRTSQLVSQI
                     CNMPIPANKAIVGSGAFAHSSGIHQDGVLKNRENYEIMTPESIGLNQIQLNLTSRSGR
                     AAVKHRMDEMGYKESEYNLDNLYDAFLKLADKKGQVFDYDLEALAFIGKQQEEPEHFR
                     LDYFSVQSGSNDIATAAVKLACGEEVKAEAANGNGPVDAVYQAINRITDYNVELVKYS
                     LTAKGHGKDALGQVDIVTNYNGRRFHGVGLATDIVESSAKAMVHVLNNIWRAAEVEKE
                     LQRKTQHNENNKETV"
     gene            7138..8229
                     /gene="leuB"
                     /locus_tag="OO357_000590"
     CDS             7138..8229
                     /gene="leuB"
                     /locus_tag="OO357_000590"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HCR5885645.1"
                     /translation="MSKNYHIAVLPGDGIGPEVMTQALKVLNAVRNRFAMRITTSHYD
                     VGGAAIDNHGQPLPPATVEGCEQADAVLFGSVGGPKWEHLPPDQQPERGALLPLRKHF
                     KLFSNLRPAKLYQGLEAFCPLRADIAANGFDIQCVRELTGGIYFGQPKGREGSGQYEK
                     AFDTEVYHRFEIERIARIAFESARKRRHKVTSIDKANVLQSSILWREIVNEIATEYPD
                     VELAHMYIDNATMQLIKDPSQFDVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQG
                     FGLYEPAGGSAPDIAGKNIANPIAQILSLALLLRYSLDADDAASAIERAINRALEEGI
                     RTGDLARGAAAVSTDEMGDIIARYVAEGV"
     gene            8232..9632
                     /gene="leuC"
                     /locus_tag="OO357_000591"
     CDS             8232..9632
                     /gene="leuC"
                     /locus_tag="OO357_000591"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase large subunit"
                     /protein_id="HCR5885646.1"
                     /translation="MAKTLYEKLFDAHVVYEAENETPLLYIDRHLVHEVTSPQAFDGL
                     RAHGRPVRQPGKTFATMDHNVSTQTKDINACGEMARIQMQELIKNCKEFGVELYDLNH
                     PYQGIVHVMGPEQGVTLPGMTIVCGDSHTATHGAFGALAFGIGTSEVEHVLATQTLKQ
                     GRAKTMKIEVQGKAAPGITAKDIVLAIIGKTGSAGGTGHVVEFCGEAIRDLSMEGRMT
                     LCNMAIEMGAKAALVAPDETTFNYVKGRLHAPKGKDFDDAVAYWKTLQTDEGATFDTV
                     VTLQAEEISPQVTWGTNPGQVISVNDNIPDPASFADPVERASAEKALAYMGLKPGIPL
                     TEVAIDKVFIGSCTNSRIEDLRAAAEIAKGRKVAPGVQALVVPGSGPVKAQAEAEGLD
                     KIFIEAGFEWRLPGCSMCLAMNNDRLNPGERCASTSNRNFEGRQGRGGRTHLVSPAMA
                     AAAAVTGHFADIRNIK"
     gene            9643..10248
                     /gene="leuD"
                     /locus_tag="OO357_000592"
     CDS             9643..10248
                     /gene="leuD"
                     /locus_tag="OO357_000592"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HCR5885647.1"
                     /translation="MAEKFIKHTGLVVPLDAANVDTDAIIPKQFLQKVTRTGFGAHLF
                     NDWRFLDEKGQQPNPDFVLNFPQYQGASILLARENFGCGSSREHAPWALTDYGFKVVI
                     APSFADIFYGNSFNNQLLPVKLSDAEVDELFALVKANPGIHFDVDLEAQEVKAGEKTY
                     RFTIDAFRRHCMMNGLDSIGLTLQHDDAIASYEAKQPAFMN"
     gene            complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OO357_000593"
     CDS             complement(10297..11475)
                     /gene="setA"
                     /locus_tag="OO357_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetA"
                     /protein_id="HCR5885648.1"
                     /translation="MIWIMTMARRMNGVYAAFMLVAFMMGVAGALQAPTLSLFLSREV
                     GAQPFWIGLFYTVNAIAGIGVSLWLAKRSDSQGDRRKLIIFCCLMAIGNALLFAFNRH
                     YLTLITCGVLLASLANTAMPQLFALAREYADNSAREVVMFSSVMRAQLSLAWVIGPPL
                     AFMLALNYGFTVMFSIAAGIFTLSLVLIAFMLLSVARVELPSENALSMQGDWQDSNVR
                     MLFVASTLMWTCNTMYIIDMPLWISSDLGLPDKLAGFLMGTAAGLEIPAMILAGYYVK
                     RYGKRRMMVIAVVAGVLFYTGLIFFHSRMALMTLQLFNAVFIGIVAGIGMLWFQDLMP
                     GRAGAATTLFTNTISTGVILAGVIQGAIAQSWGHFAVYWVIAIISVVALFLTAKVKDV
                     "
     gene            complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OO357_000594"
     CDS             complement(11577..11708)
                     /gene="sgrT"
                     /locus_tag="OO357_000594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake inhibitor SgrT"
                     /protein_id="HCR5885649.1"
                     /translation="MRQFYQHYFTATAKLCWLRWLSVPQRLTMLEGLMQWDDRNSES"
     gene            11797..13452
                     /gene="sgrR"
                     /locus_tag="OO357_000595"
     CDS             11797..13452
                     /gene="sgrR"
                     /locus_tag="OO357_000595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator SgrR"
                     /protein_id="HCR5885650.1"
                     /translation="MPSARLQQQFIRLWQCCEGKSQDTTLNELAALLSCSRRHMRTLL
                     NTMQDRGWLTWEAEVGRGKRSRLTFLYTGLALQQQRAEDLLEQDRIDQLVQLVGDKAT
                     VRQMLVSHLGRSFRQGRHILRVLYYRPLRNLLPGSALRRSETHIARQIFSSLTRINEE
                     NGELEADIAHHWQQISPLHWRFFLRPGVHFHHGRELEMDDVIASLKRINTLPLYSHIT
                     DIVSPTPWTLDIHLTQPDRWLPLLLGQVPAMILPCEWETLSNFASHPIGTGPYAVIRN
                     STNQLKIQAFDDFFGYRALIDEVNVWVLPEIADEPAGGLMLKGPQGEKKEIESRLEEG
                     CYYLLFDSRTHRGANQQVRDWVSYVLSPTNLVYFAEEQYQQLWFPAYGLLPRWHHART
                     IKSEKPAGLESLTLTFYQDHSEHRVIAGIMQQILASHQVTLEIKEISYDQWHEGEIES
                     DIWLNSANFTLPLDFSLFAHLCEVPLLQHCIPIDWQADAARWRNGEMNLANWCQQLVA
                     SKAMVPLIHHWLIIQGQRSMRGLRMNTLGWFDFKSAWFAPPDP"
     regulatory      13486..13585
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            13616..14599
                     /gene="thiB"
                     /locus_tag="OO357_000596"
     CDS             13616..14599
                     /gene="thiB"
                     /locus_tag="OO357_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate binding
                     subunit"
                     /protein_id="HCR5885651.1"
                     /translation="MLKKCLPLLLLCTAPVFAKPVLTVYTYDSFAADWGPGPKIKKAF
                     EANCNCELKLVALEDGVSLLNRLRMEGKNSKADVVLGLDNNLLDAASKTGLFAKSGVA
                     ADAVNVPGGWNNDTFVPFDYGYFAFVYDKNKLKNPPQSLKELVESDQNWRVIYQDPRT
                     STPGLGLLLWMQKVYGDDAPQAWQKLAKKTVTVTKGWSEAYGLFLKGESDLVLSYTTS
                     PAYHILEEKKDNYAAANFSEGHYLQVEVAARTAASKQPELAQKFLQFMVSPAFQNAIP
                     TGNWMYPVANVTLPAGFEQLTKPATTLEFTPAEVAAQRQAWISEWQRAVSR"
     gene            14575..16185
                     /gene="thiP"
                     /locus_tag="OO357_000597"
     CDS             14575..16185
                     /gene="thiP"
                     /locus_tag="OO357_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine/thiamine pyrophosphate ABC transporter
                     permease ThiP"
                     /protein_id="HCR5885652.1"
                     /translation="MATRRQPLIPGWLIPGVSAATLVVAVALAAFLALWWNAPQGNWV
                     AVWQDSYLWHVVRFSFWQAFLSALLSVVPAIFLARALYRRRFPGRLALLRLCAMTLIL
                     PVLVAVFGILSVYGRQGWLASLCQSLGLEWTFSPYGLQGILLAHVFFNLPMASRLLLQ
                     ALENIPGEQRQLAAQLGMRGWHFFRFVEWPWLRRQIPPVAALIFMLCFASFATVLSLG
                     GGPQATTIELAIYQALSYDYDPARAAMLALLQMVCCLGLVLLSQRLSKAIAPGTTLLQ
                     GWRDPDDRLHSRICDTVLIVLALLLLLPPLLAVIVDGVNRQLPEVLAQPVLWQALWTS
                     LRIALAAGVLCVVLTMMLLWSSRELRARQKMLAGQALEMSGMLILAMPGIVLATGFFL
                     LLNNTIGLPQSADGIVIFTNALMAIPYALKVLENPMRDITARYSMLCQSLGIEGWSRL
                     KVVELRALKRPLAQALAFACVLSIGDFGVVALFGNDDFRTLPFYLYQQIGSYRSQDGA
                     VTALILLLLCFLLFTVIEKLPGRNVKTD"
     gene            16169..16867
                     /gene="thiQ"
                     /locus_tag="OO357_000598"
     CDS             16169..16867
                     /gene="thiQ"
                     /locus_tag="OO357_000598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter ATP-binding protein
                     ThiQ"
                     /protein_id="HCR5885653.1"
                     /translation="MLKLTDITWLYHHLPMRFSLTVERGEQVAILGPSGAGKSTLLNL
                     IAGFLTPASGSLTIDGVDHTTTPPSRRPVSMLFQENNLFSHLTVAQNIGLGLNPGLKL
                     NAAQQEKMHAIARQMGIDNLMARLPGELSGGQRQRVALARCLVREQPILLLDEPFSAL
                     DPALRQEMLTLVSTSCQQQKMTLLMVSHSVEDAARIATRSVVVADGRIAWQGKTEELL
                     SGKASASAILGITG"
     gene            complement(16981..17745)
                     /locus_tag="OO357_000599"
     CDS             complement(16981..17745)
                     /locus_tag="OO357_000599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5885654.1"
                     /translation="MQALLEHFITQSTVYSLMAVVLVAFLESLALVGLILPGTVLMAG
                     LGALIGSGELSFWHAWLAGIVGCLLGDWISFWLGWRFKKPLHRWSFLKKNKALLDKTE
                     HALHQHSMFTILVGRFVGPTRPLVPMVAGMLDLPVAKFITPNIIGCLLWPPFYFLPGI
                     LAGAAIDIPAGMQSGEFKWLLLATAVFLWVGGWLCWRLWRSGKATDRLSHYLSRGRLL
                     WLTPLISAIGVVALVVLIRHPLMPVYIDILRKVVGV"
     gene            complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OO357_000600"
     CDS             complement(17831..18709)
                     /gene="araC"
                     /locus_tag="OO357_000600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706017.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose operon transcriptional regulator AraC"
                     /protein_id="HCR5885655.1"
                     /translation="MAEAQNDPLLPGYSFNAHLVAGLTPIEANGYLDFFIDRPLGMKG
                     YILNLTIRGQGVVKNQGREFVCRPGDILLFPPGEIHHYGCHPEAREWYHQWVYFRPRA
                     YWHEWLNWPSIFANTGFFRPDEAHQPYFSDLFGQIINAGQGEGRYSELLAMNLLEQLL
                     LRRMEAINESLHPPMDNRVREACQYISDHLADSNFDIASVAQHVCLSPSRLSHLFRQQ
                     LGISVLSWREDQRISQAKLLLSTTRMPIATVGRNVGFDDQLYFSRVFKKCTGASPSEF
                     RAGCEEKVNDVAVKLS"
     gene            19048..20748
                     /gene="araB"
                     /locus_tag="OO357_000601"
     CDS             19048..20748
                     /gene="araB"
                     /locus_tag="OO357_000601"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HCR5885656.1"
                     /translation="MAIAIGLDFGSDSVRALAVDCASGEEIATSVEWYPRWQKGQFCD
                     APNNQFRHHPRDYIESMEAALKTVLAELSVEQRAAVVGIGVDSTGSTPAPIDADGNVL
                     ALRPEFAENPNAMFVLWKDHTAVEEAEEITRLCHAPGNVDYSRYIGGIYSSEWFWAKI
                     LHVTRQDSAVAQSAASWIELCDWVPALLSGTTRPQDIRRGRCSAGHKSLWHESWGGLP
                     PASFFDELDPILNRHLPSPLFTDTWTADIPVGTLCPEWAQRLGLPESVVISGGAFDCH
                     MGAVGAGAQPNALVKVIGTSTCDILIADKQSVGERAVKGICGQVDGSVVPGFIGLEAG
                     QSAFGDIYAWFGRVLGWPLEQLAAQHPELKAQINASQKQLLPALTEAWAKNPSLDHLP
                     VVLDWFNGRRTPNANQRLKGVITDLNLATDTPLLFGGLIAATAFGARAIMECFTDQGI
                     AVNNVMALGGIARKNQVIMQACCDVLNRPLQIVASDQCCALGAAIFAAVAAKVHADIP
                     SAQQKMASAVEKTLQPRSEQAQRFEQLYRRYQQWAMSAEQHYLPTSAPAQAAQAVATL
                     "
     gene            20759..22261
                     /gene="araA"
                     /locus_tag="OO357_000602"
     CDS             20759..22261
                     /gene="araA"
                     /locus_tag="OO357_000602"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HCR5885657.1"
                     /translation="MAIFDNYEVWFVIGSQHLYGPETLRQVTQHAEHVVNALNTEAKL
                     PYKLVLKPLGTTPDEITAICRDANYDDRCAGLVVWLHTFSPAKMWINGLTMLNKPLLQ
                     FHTQFNAALPWDSIDMDFMNLNQTAHGGREFGFIGARMRQQHAVVTGHWQDKQAHERI
                     GSWMRQAVSKQDTRHLKVCRFGDNMREVAVTDGDKVAAQIKFGFSVNTWAVGYLVQVV
                     NSISDGDVNALVDEYESCYTMTPATQIHGEKRQNVLEAARIELGMKRFLEQGGFHAFT
                     TTFEDLHGLKQLPGLPVQRLMQQGYGFAGEGDWKTAALLRIMKVMSTGLQGGTSFMED
                     YTYHFEKGNDLVLGSHMLEVCPSIAVEEKPILDVQHLGIGGKDDPARLIFNTQTGPAI
                     VASLIDLGDRYRLLVNCIDTVKTPHSLPKLPVANALWKAQPDLPTASEAWILAGGAHH
                     TVFSHALNLNDMRQFAEMHDIEITVIDNDTRLPAFKDALRWNEVYYGFRR"
     gene            22461..23156
                     /gene="araD"
                     /locus_tag="OO357_000603"
     CDS             22461..23156
                     /gene="araD"
                     /locus_tag="OO357_000603"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HCR5885658.1"
                     /translation="MLKDLKRQVLEANLALPKHNLVTLTWGNVSAVDRERGVFVIKPS
                     GVDYSVMTADDMVVVSIETGEVVEGTKKPSSDTPTHRLLYQAFPSIGGIVHTHSRHAT
                     IWAQAGQSIPATGTTHADYFYGTIPCTRKMTDAEINGEYEWETGNVIVETFEKQGIDA
                     AQMPGVLVHSHGPFAWGKNAEDAVHNAIVLEEVAYMGIFCRQLAPQLPDMQQTLLDKH
                     YLRKHGAKAYYGQ"
     gene            23231..25582
                     /gene="polB"
                     /locus_tag="OO357_000604"
     CDS             23231..25582
                     /gene="polB"
                     /locus_tag="OO357_000604"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase II"
                     /protein_id="HCR5885659.1"
                     /translation="MAQAGFILTRHWRDTPQGTEVSFWLATDNGPLQVTLAPQESVAF
                     IHADQVPRAQHILQGEQGFRLTPLALKDFHRQPVYGLYCRAHRQLMNYEKRLREGGVT
                     VYEADVRPPERYLMERFITSPVWVEGDMHNGTIVNARLKPHPDYRPPLKWVSIDIETT
                     RHGELYCIGLEGCGQRIVYMLGPENGDASSLDFELEYVASRPLLLEKLNAWFANHDPD
                     VIIGWNVVQFDLRMLQKHAERYRLPLRLGRDNSELEWREHGFKNGVFFAQAKGRLIID
                     GIEALKSAFWNFSSFSLETVAQELLGEGKSIDNPWDRMDEIDRRFAEDKPALATYNLK
                     DCELVTQIFHKTEIMPFLLERATVNGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLG
                     EVPPHASPGGYVMDSRPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHS
                     TEGFLDAWFSREKHCLPEIVTNIWHGRDKAKRQGNKPLSQALKIIMNAFYGVLGTTAC
                     RFFDPRLASSITMRGHQIMRQTKALIEAQGYDIIYGDTDSTFVWLKGAHSEEEAAKIG
                     RALVQHVNAWWAETLQKQRLTSALELEYETHFCRFLMPTIRGADTGSKKRYAGLIQEG
                     DKQRMVFKGLETVRTDWTPLAQQFQQELYLRIFRNEPYQEYVRQTIDKLMAGELDARL
                     VYRKRLRRPLSEYQRNVPPHVRAARLADEENQKRGRPLQYQNRGTIKYVWTTNGPEPL
                     DYQRSPLDYEHYLTRQLQPVAEGILPFIEDNFATLMTGQLGLF"
     gene            25747..28653
                     /gene="rapA"
                     /locus_tag="OO357_000605"
     CDS             25747..28653
                     /gene="rapA"
                     /locus_tag="OO357_000605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001117023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-associated protein RapA"
                     /protein_id="HCR5885660.1"
                     /translation="MPFTLGQRWISDTESELGLGTVVAVDARTVTLLFPSTGENRLYA
                     RSDSPVTRVMFNPGDTITSHDGWQMQVEEVKEENGLLTYIGTRLDTEESGVALREVFL
                     DSKLVFSKPQDRLFAGQIDRMDRFALRYRARKYSSEQFRMPYSGLRGQRTSLIPHQLN
                     IAHDVGRRHAPRVLLADEVGLGKTIEAGMILHQQLLSGAAERVLIIVPETLQHQWLVE
                     MLRRFNLRFALFDDERYAEAQHDAYNPFDTEQLVICSLDFARRSKQRLEHLCEAEWDL
                     LVVDEAHHLVWSEDAPSREYQAIEQLAEHVPGVLLLTATPEQLGMESHFARLRLLDPN
                     RFHDFAQFVEEQKNYRPVADAVAMLLAGNKLSNDELNMLGEMIGEQDIEPLLQAANSD
                     SEDAQSARQELVSMLMDRHGTSRVLFRNTRNGVKGFPKRELHTIKLPLPTQYQTAIKV
                     SGIMGARKSAEDRARDMLYPERIYQEFEGDNATWWNFDPRVEWLMGYLTSHRSQKVLV
                     ICAKAATALQLEQVLREREGIRAAVFHEGMSIIERDRAAAWFAEEDTGAQVLLCSEIG
                     SEGRNFQFASHMVMFDLPFNPDLLEQRIGRLDRIGQAHDIQIHVPYLEKTAQSVLVRW
                     YHEGLDAFEHTCPTGRTIYDSVYNDLINYLASPDQTEGFDDLIKSCREQHEALKAQLE
                     QGRDRLLEIHSNGGEKAQALAESIEEQDDDTNLIAFAMNLFDIIGINQDDRGDNMIVL
                     TPSDHMLVPDFPGLSEDGITITFDREVALAREDAQFITWEHPLIRNGLDLILSGDTGS
                     STISLLKNKALPVGTLLVELIYVVEAQAPKQLQLNRFLPPTPVRMLLDKNGNNLAAQV
                     EFETFNRQLNAVNRHTGSKLVNAVQQDVHAILQLGEAQIEKSARALIDAARNEADEKL
                     SAELSRLEALRAVNPNIRDDELTAIESNRQQVMESLDQAGWRLDALRLIVVTHQ"
     gene            28665..29324
                     /gene="rluA"
                     /locus_tag="OO357_000606"
     CDS             28665..29324
                     /gene="rluA"
                     /locus_tag="OO357_000606"
                     /EC_number="5.4.99.28"
                     /EC_number="5.4.99.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA pseudouridine(32) synthase/23S
                     rRNA pseudouridine(746) synthase RluA"
                     /protein_id="HCR5885661.1"
                     /translation="MGMENYNPPQEPWLVILYQDDHIMVVNKPSGLLSVPGRLEEHKD
                     SVMTRIQRDYPQAESVHRLDMATSGVIVVALTKAAERELKRQFREREPKKQYVARVWG
                     HPSPAEGLVDLPLICDWPNRPKQKVCYETGKPAQTEYEVVEYAADNTARVVLKPITGR
                     SHQLRVHMLALGHPILGDRFYASPEARAMAPRLLLHAEMLTITHPAYGNSMTFKAPAD
                     F"
     gene            complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OO357_000607"
     CDS             complement(29441..30256)
                     /gene="djlA"
                     /locus_tag="OO357_000607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone DjlA"
                     /protein_id="HCR5885662.1"
                     /translation="MQYWGKIIGVAVALLMGGGFWGVVLGLLIGHMFDKARSRKMAWF
                     ANQRERQALFFATTFEVMGHLTKSKGRVTEADIHIASQLMDRMNLHGASRTAAQNAFR
                     VGKSDNYPLREKMRQFRSVCFGRFDLIRMFLEIQIQAAFADGSLHPNERAVLYVIAEE
                     LGISRAQFDQFLRMMQGGAQFGGGYQQQSGGGNWQQAQRGPTLEDACNVLGVKPTDDA
                     TTIKRAYRKLMSEHHPDKLVAKGLPPEMMEMAKQKAQEIQQAYELIKQQKGFK"
     gene            30511..32865
                     /gene="lptD"
                     /locus_tag="OO357_000608"
     CDS             30511..32865
                     /gene="lptD"
                     /locus_tag="OO357_000608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly protein LptD"
                     /protein_id="HCR5885663.1"
                     /translation="MKKRIPTLLATMIATALYSQQGLAADLASQCMLGVPSYDRPLVQ
                     GDTNDLPVTINADHAKGDYPDDAVFTGSVDIMQGNSRLQADEVQLHQKEAPGQPEPVR
                     TVDALGNVHYDDNQVILKGPKGWANLNTKDTNVWEGDYQMVGRQGRGKADLMKQRGEN
                     RYTILDNGSFTSCLPGSDTWSVVGSEIIHDREEQVAEIWNARFKVGPVPIFYSPYLQL
                     PVGDKRRSGFLIPNAKYTTTNYFEFYLPYYWNIAPNMDATITPHYMHRRGNIMWENEF
                     RYLSQAGAGLMELDYLPSDKVYEDEHPNDDSSRRWLFYWNHSGVMDQVWRFNVDYTKV
                     SDPSYFNDFDNKYGSSTDGYATQKFSVGYAVQNFNATVSTKQFQVFSEQNTSSYSAEP
                     QLDVNYYQNDVGPFDTRIYGQAVHFVNTRDDMPEATRVHLEPTINLPLSNNWGSINTE
                     AKFLATHYQQTNLDWYNSRNTTKLDESVNRVMPQFKVDGKMVFERDMEMLAPGYTQTL
                     EPRAQYLYVPYRDQSDIYNYDSSLLQSDYSGLFRDRTYGGLDRIASANQVTTGVTSRI
                     YDDAAVERFNISVGQIYYFTESRTGDDNITWENDDKTGSLVWAGDTYWRISERWGLRG
                     GIQYDTRLDNVATSNSSIEYRRDEDRLVQLNYHYASPEYIQATLPKYYSTAEQYKNGI
                     SQVGAVASRPIADRWSIVGAYYYDTNANKQADSMLGVQYSSCCYAIRVGYERKLNGWD
                     NDKQHAVYDNAIGFNIELRGLSSNYGLGTQEMLRSNILPYQNTL"
     gene            32918..34204
                     /gene="surA"
                     /locus_tag="OO357_000609"
     CDS             32918..34204
                     /gene="surA"
                     /locus_tag="OO357_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase SurA"
                     /protein_id="HCR5885664.1"
                     /translation="MKNWKTLLLGIAMIANTSFAAPQVVDKVAAVVNNGVVLESDVDG
                     LMQSVKLNAAQARQQLPDDATLRHQIMERLIMDQIILQMGQKMGVKISDEQLDQAIAN
                     IAKQNNMTLDQMRSRLAYDGLNYNTYRNQIRKEMIISEVRNNEVRRRITILPQEVESL
                     AQQVGNQNDASTELNLSHILIPLPENPTSDQVNEAESQARAIVDQARNGADFGKLAIA
                     HSADQQALNGGQMGWGRIQELPGIFAQALSTAKKGDIVGPIRSGVGFHILKVNDLRGE
                     SKNISVTEVHARHILLKPSPIMTDEQARVKLEQIAADIKSGKTTFAAAAKEFSQDPGS
                     ANQGGDLGWATPDIFDPAFRDALTRLNKGQMSAPVHSSFGWHLIELLDTRNVDKTDAA
                     QKDRAYRMLMNRKFSEEAASWMQEQRASAYVKILSN"
     gene            34204..35193
                     /gene="pdxA"
                     /locus_tag="OO357_000610"
     CDS             34204..35193
                     /gene="pdxA"
                     /locus_tag="OO357_000610"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HCR5885665.1"
                     /translation="MVKTQRVVITPGEPAGIGPDLVVQLAQREWPVELVVCADATLLT
                     DRAAMLGLPLTLRPYSPNSPAQPQTTGTLTLLPVALRESVTAGQLAIENGHYVVETLA
                     RACDGCLNGEFAALITGPVHKGVINDASIPFTGHTEFFEERSQAKKVVMMLATEELRV
                     ALATTHLPLRDIADAITPALLHEVIAILHHDLRTKFGIAEPRILVCGLNPHAGEGGHM
                     GTEEIDTIIPVLDELRAQGMKLNGPLPADTLFQPKYLDNADAVLAMYHDQGLPVLKYQ
                     GFGRGVNITLGLPFIRTSVDHGTALELAGRGEADVGSFITALNLAIKMIVNTQ"
     gene            35190..36011
                     /gene="rsmA"
                     /locus_tag="OO357_000611"
     CDS             35190..36011
                     /gene="rsmA"
                     /locus_tag="OO357_000611"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HCR5885666.1"
                     /translation="MNNRVHQGHLARKRFGQNFLNDQFVIDSIVSAINPQKGQAMVEI
                     GPGLAALTEPVGERLDQLTVIELDRDLAARLQTHPFLGPKLTIYQQDAMTFNFGELAE
                     KMGQPLRVFGNLPYNISTPLMFHLFSYTDAIADMHFMLQKEVVNRLVAGPNSKAYGRL
                     SVMAQYYCNVIPVLEVPPSAFTPPPKVDSAVVRLVPHATMPHPVKDIRVLSRITTEAF
                     NQRRKTIRNSLGNLFSVEVLTGMGIDPAMRAENISVAQYCQMANYLAENAPLQES"
     gene            36014..36391
                     /gene="apaG"
                     /locus_tag="OO357_000612"
     CDS             36014..36391
                     /gene="apaG"
                     /locus_tag="OO357_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HCR5885667.1"
                     /translation="MINSPRVCIQVQSVYIEAQSSPDNERYVFAYTVTIRNLGRAPVQ
                     LLGRYWLITNGNGRETEVQGEGVVGVQPLIAPGEEYQYTSGAIIETPLGTMQGHYEMI
                     DENGVPFSIDIPVFRLAVPTLIH"
     gene            36398..37240
                     /gene="apaH"
                     /locus_tag="OO357_000613"
     CDS             36398..37240
                     /gene="apaH"
                     /locus_tag="OO357_000613"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     ApaH"
                     /protein_id="HCR5885668.1"
                     /translation="MATYLIGDVHGCYDELIALLHKVEFTPGKDTLWLTGDLVARGPG
                     SLDVLRYVKSLGDSVRLVLGNHDLHLLAVFAGISRNKPKDRLTPLLEAPDADELLNWL
                     RRQPLLQIDEEKKLVMAHAGITPQWDLQTAKECARDVEAVLSSDSYPFFLDAMYGDMP
                     NNWSPELRGLGRLRFITNAFTRMRFCFPNGQLDMYSKESPEEAPAPLKPWFAIPGPVA
                     EEYSIAFGHWASLEGKGTPEGIYALDTGCCWGGSLTCLRWEDKQYFVQPSNRHKDLGE
                     AAAS"
     gene            complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OO357_000614"
     CDS             complement(37318..37797)
                     /gene="folA"
                     /locus_tag="OO357_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 dihydrofolate reductase"
                     /protein_id="HCR5885669.1"
                     /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR
                     HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ
                     FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     gene            complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OO357_000615"
     CDS             complement(37988..39850)
                     /gene="kefC"
                     /locus_tag="OO357_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     protein KefC"
                     /protein_id="HCR5885670.1"
                     /translation="MDSHTLVQALIYLGSAALIVPIAVRLGLGSVLGYLIAGCIIGPW
                     GLRLVTDAESILHFAEIGVVLMLFIIGLELDPQRLWKLRAAVFGGGALQMVICGGLLG
                     LFCMLLGLRWQVAELIGMTLALSSTAIAMQAMNERNLMVTQMGRSAFAVLLFQDIAAI
                     PLVAMIPLLAASSASTTMGAFALSALKVAGALVLVVLLGRYVTRPALRFVARSGLREV
                     FSAVALFLVFGFGLLLEEVGLSMAMGAFLAGVLLASSEYRHALESDIEPFKGLLLGLF
                     FIGVGMSIDFGTLIENPLRIVILLLGFLIIKIAMLWLIARPLQVPNKQRRWFAVLLGQ
                     GSEFAFVVFGAAQMANVLEPEWAKSLTLAVALSMAATPILLVILNRLEQSSTEEAREA
                     DEIDEEQPRVIIAGFGRFGQITGRLLLSSGVKMVVLDHDPDHIETLRKFGMKVFYGDA
                     TRMDLLESAGAAKAEVLINAIDDPQTNLQLTEMVKEHFPHLQIIARARDVDHYIRLRQ
                     AGVEKPERETFEGALKTGRLALESLGLGPYEARERADVFRRFNIQMVEEMAMVENDTK
                     ARAAVYKRTSAMLSEIITEDREHLSLIQRHGWQGTEEGKHTGNMADEPETKPSS"
     gene            complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OO357_000616"
     CDS             complement(39843..40373)
                     /gene="kefF"
                     /locus_tag="OO357_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-regulated potassium-efflux system
                     oxidoreductase KefF"
                     /protein_id="HCR5885671.1"
                     /translation="MILIIYAHPYPHHSHANKRMLEQARTLEGVEIRSLYQLYPDFNI
                     DIAAEQEALSRADLIVWQHPMQWYSIPPLLKLWIDKVLSHGWAYGHGGTALHGKHLLW
                     AVTTGGGESHFEIGAHPGFDVLSQPLQATAIYCGLNWLPPFAMHCTFICDDETLEGQA
                     RHYKQRLLEWQEAHHG"
     gene            complement(40421..41815)
                     /locus_tag="OO357_000617"
     CDS             complement(40421..41815)
                     /locus_tag="OO357_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5885672.1"
                     /translation="MQPSRNFDDLKFSSIHRRILLWGSGGPFLDGYVLVMIGVALEQL
                     TPALKLDADWIGLLGAGTLAGLFVGTSLFGYISDKVGRRKMFLIDIIAIGVISVATMC
                     VSSPVELLVMRVLIGIVIGADYPIATSMITEFSSTRQRAFSISFIAAMWYVGATCADL
                     VGYWLYDVEGGWRWMLGSAAIPCLLILIGRFELPESPRWLLRKGRVKECEEMMIKLFG
                     EPVAFDEEQPQQTRFRDLFNRRHFPFVLFVAAIWTCQVIPMFAIYTFGPQIVGSLGLG
                     VGKNAALGNVVISLFFMLGCIPPMLWLNTAGRRPLLIGSFAMMTLALAVLGLIPDMGI
                     WLVVMAFAVYAFFSGGPGNLQWLYPNELFPTDIRASAVGVIMSLSRIGTIVSTWALPI
                     FINNYGISNTMLMGAGISLFGLLISVAFAPETRGMSLAQTSNMTIRGQRMGLFVQISL
                     FSESILLTISRVNI"
     gene            complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="OO357_000618"
     CDS             complement(41874..42161)
                     /gene="fixX"
                     /locus_tag="OO357_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein FixX"
                     /protein_id="HCR5885673.1"
                     /translation="MTSPVNVDVKLGVNKFNVDEEHPHIVVKADADKQVLELLVKACP
                     AGLYKKQDDGSVRFDYAGCLECGTCRILGLGSALEQWEYPRGTFGVEFRYG"
     gene            complement(42158..43444)
                     /locus_tag="OO357_000619"
     CDS             complement(42158..43444)
                     /locus_tag="OO357_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5885674.1"
                     /translation="MSEDIFDAIIVGAGLAGSVAALVLAREGAQVLVIERGNSAGAKN
                     VTGGRLYAHSLEHIIPGFADSAPVERLITHEKLAFMTEKSAMTMDYCNGDETSPSQRS
                     YSVLRSKFDAWLMEQAEEAGAQLITGIRVDNLVQRDGKVVGVEADGDVIEAKTVLLAD
                     GVNSILAEKLGMAKRVKPTDVAVGVKELIELPKSVIEDRFQLQGNQGAACLFAGSPTD
                     GLMGGGFLYTNENTLSLGLVCGLHHLHDAKKSVPQMLEDFKQHPAVAPLIAGGKLVEY
                     SAHVVPEAGINMLPELVGDGVLIAGDAAGMCMNLGFTIRGMDLAIAAGEAAAKTVLSA
                     MKSDDFSKQKLAEYRQHLESGPLRDMRMYQKLPAFLDNPRMFSGNPELAVGVARDLFT
                     IDGSAPELMRKKILRHGKKVGFINLIKDGMKGVTVL"
     gene            complement(43495..44436)
                     /locus_tag="OO357_000620"
                     /pseudo
     CDS             complement(43495..44436)
                     /locus_tag="OO357_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308072.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
     gene            complement(44451..45221)
                     /locus_tag="OO357_000621"
     CDS             complement(44451..45221)
                     /locus_tag="OO357_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein FixA"
                     /protein_id="HCR5885675.1"
                     /translation="MKIITCYKCVPDEQDIAVNNADGSLDFSKADAKISQYDLNAIEA
                     ACQLKQQAAEAQVTALSVGGKALTNAKGRKDVLSRGPDELIVVIDDQFEQALPQQTAS
                     ALAAAAQKAGFDLILCGDGSSDLYAQQVGLLVGEILNIPAVNGVSKIISLTADTLTVE
                     RELEDETETLSIPLPAVVAVSTDINSPQIPSMKAILGAAKKPVQVWSAADIGFNAVDA
                     WSEQQVAAPKQRERQRIVIEGDGEEQIAAFAENLRKVI"
     gene            45577..47091
                     /gene="caiT"
                     /locus_tag="OO357_000622"
     CDS             45577..47091
                     /gene="caiT"
                     /locus_tag="OO357_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine/gamma-butyrobetaine antiport BCCT
                     transporter"
                     /protein_id="HCR5885676.1"
                     /translation="MKNEKRKTGIEPKVFFPPLIIVGILCWLTVRDLDAANVVINAVF
                     SYVTNVWGWAFEWYMVVMLFGWFWLVFGPYAKKRLGNEPPEFSTASWIFMMFASCTSA
                     AVLFWGSIEIYYYISTPPFGLEPNSTGAKELGLAYSLFHWGPLPWATYSFLSVAFAYF
                     FFVRKMEVIRPSSTLVPLAGEKHAKGLFGTIVDNFYLVALIFAMGTSLGLATPLVTEC
                     MQWLFGIPHTLQLDAIIITCWIILNAICVACGLQKGVRIASDVRSYLSFLMLGWVFIV
                     SGASFIMNYFTDSVGMLLMYLPRMLFYTDPIAKGGFPQGWTVFYWAWWVIYAIQMSIF
                     LARISRGRTVRELCFGMVMGLTASTWILWTVLGSNTLLLMDKNIINIPNLIEQYGVAR
                     AIIETWAALPLSTATMWGFFILCFIATVTLVNACSYTLAMSTCREVRDGEEPPLLVRI
                     GWSILVGIIGIVLLALGGLKPIQTAIIAGGCPLFFVNIMVTLSFIKDAKQNWKD"
     gene            47122..48264
                     /gene="caiA"
                     /locus_tag="OO357_000623"
     CDS             47122..48264
                     /gene="caiA"
                     /locus_tag="OO357_000623"
                     /EC_number="1.3.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA dehydrogenase"
                     /protein_id="HCR5885677.1"
                     /translation="MDFNLNDEQELFVAGIRELMASENWEAYFAECDRDSVYPERFVK
                     ALADMGIDSLLIPEEHGGLDAGFVTLAAVWMELGRLGAPTYVLYQLPGGFNTFLREGT
                     QEQIDKIMAFRGTGKQMWNSAITEPGAGSDVGSLKTTYTRRNGKIYLNGSKCFITSSA
                     YTPYIVVMARDGASPDKPVYTEWFVDMSKPGIKVTKLEKLGLRMDSCCEITFDDVELD
                     EKDMFGREGNGFNRVKEEFDHERFLVALTNYGTAMCAFEDAARYANQRVQFGEAIGRF
                     QLIQEKFAHMAIKLNSMKNMLYEAAWKADNGTITSGDAAMCKYFCANAAFEVVDSAMQ
                     VLGGVGIAGNHRISRFWRDLRVDRVSGGSDEMQILTLGRAVLKQYR"
     gene            48393..49610
                     /gene="caiB"
                     /locus_tag="OO357_000624"
     CDS             48393..49610
                     /gene="caiB"
                     /locus_tag="OO357_000624"
                     /EC_number="2.8.3.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-carnitine CoA-transferase"
                     /protein_id="HCR5885678.1"
                     /translation="MDHLPMPKFGPLAGLRVVFSGIEIAGPFAGQMFAEWGAEVIWIE
                     NVAWADTIRVQPNYPQLSRRNLHALSLNIFKDEGREAFLKLMETTDIFIEASKGPAFA
                     RRGITDEVLWQHNPKLVIAHLSGFGQYGTEEYTNLPAYNTIAQAFSGYLIQNGDVDQP
                     MPAFPYTADYFSGLTATTAALAALHKVRETGKGESIDIAMYEVMLRMGQYFMMDYFNG
                     GEMCPRMSKGKDPYYAGCGLYKCADGYIVMELVGITQIEECFKDIGLAHLLGTPEIPE
                     GTQLIHRIECPYGPLVEEKLDAWLAAHTIAEVKERFAELNIACAKVLTVPELESNPQY
                     VARESITQWQTMDGRTCKGPNIMPKFKNNPGQIWRGMPSHGMDTAAILKNIGYSENDI
                     QELVSKGLAKVED"
     gene            49684..51237
                     /gene="caiC"
                     /locus_tag="OO357_000625"
     CDS             49684..51237
                     /gene="caiC"
                     /locus_tag="OO357_000625"
                     /EC_number="6.2.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetaine/carnitine-CoA ligase"
                     /protein_id="HCR5885679.1"
                     /translation="MDIIGGQHLRQMWDDLADVYGHKTALICESSGGVVNRYSYLELN
                     QEINRTANLFYTLGIRKGNKVALHLDNCPEFIFCWFGLAKIGAIMVPINARLLREESE
                     WILQNSQACLLVTSAQFYPMYQQIQQEDATQLRHICLTDVALPADDGVSSFTQLKNQQ
                     PATLCYAPPLSTDDTAEILFTSGTTSRPKGVVITHYNLRFAGYYSAWQCALRDDDVYM
                     TVMPAFHIDCQCTAAMAAFSAGATFVLVEKYSARAFWGQAQKYRATITECIPMMIRTL
                     MVQPPSANDRQHRLREVMFYLNLSEQEKDAFCERFGVRLLTSYGMTETIVGIIGDRPS
                     DKRRWPSIGRAGFCYEAEIRDDHNRPLPAGEIGEICIKGVPGKTIFKEYFLNPKATAK
                     VLEADGWLHTGDTGYRDEEGFFYFVDRRCNMIKRGGENVSCVELENIIATHPKIQDIV
                     VVGIKDSIRDEAIKAFVVLNEGETLSEEEFFRFCEQNMAKFKVPSYLEIRKDLPRNCS
                     GKIIRKNLK"
     gene            51346..52131
                     /gene="caiD"
                     /locus_tag="OO357_000626"
     CDS             51346..52131
                     /gene="caiD"
                     /locus_tag="OO357_000626"
                     /EC_number="4.2.1.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonobetainyl-CoA hydratase"
                     /protein_id="HCR5885680.1"
                     /translation="MSESLHLTRNGSILEITLDRPKANAIDAKTSFEMGEVFLNFRDD
                     PQLRVAIITGAGEKFFSAGWDLKAAAEGEAPDADFGPGGFAGLTEIFNLDKPVIAAVN
                     GYAFGGGFELALAADFIVCADNASFALPEAKLGIVPDSGGVLRLPKILPPTIVNEMVM
                     TGRRMGAEEALRWGIVNRVVSQAELMDNARELAQQLVNSAPLAIAALKEIYRTTSEMP
                     VEEAYRYIRSGVLKHYPSVLHSEDAIEGPLAFAEKRDPVWKGR"
     gene            52137..52727
                     /gene="caiE"
                     /locus_tag="OO357_000627"
     CDS             52137..52727
                     /gene="caiE"
                     /locus_tag="OO357_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine operon protein CaiE"
                     /protein_id="HCR5885681.1"
                     /translation="MSYYAFEGLIPVVHPTAFVHPSAVLIGDVIVGAGVYIGPLASLR
                     GDYGRLIVQAGANIQDGCIMHGYCDTDTIVGENGHIGHGAILHGCVIGRDALVGMNSV
                     IMDGAVIGEESIVAAMSFVKAGFSGEKRQLLMGTPARAVRSVSDDELHWKRLNTKEYQ
                     DLVGRCHASLHETQPLRQMEENRPRLQGTTDVTPKR"
     gene            complement(52813..53208)
                     /gene="caiF"
                     /locus_tag="OO357_000628"
     CDS             complement(52813..53208)
                     /gene="caiF"
                     /locus_tag="OO357_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414576.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine metabolism transcriptional regulator
                     CaiF"
                     /protein_id="HCR5885682.1"
                     /translation="MCEGYVEKPLYLLIAEWMMAENRWVIAREISIHFDIEHSKAVNT
                     LTYILSEVTEISCEVKMIPNKLEGRGCQCQRLVKVVDIDEQIYARLRNNSREKLVGVR
                     KTPRIPAVPLTELNREQKWQMMLSKSMRR"
     gene            complement(53470..56691)
                     /gene="carB"
                     /locus_tag="OO357_000629"
     CDS             complement(53470..56691)
                     /gene="carB"
                     /locus_tag="OO357_000629"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HCR5885683.1"
                     /translation="MPKRTDIKSILILGAGPIVIGQACEFDYSGAQACKALREEGYRV
                     ILVNSNPATIMTDPEMADATYIEPIHWEVVRKIIEKERPDAVLPTMGGQTALNCALEL
                     ERQGVLEEFGVTMIGATADAIDKAEDRRRFDVAMKKIGLETARSGIAHTMEEALAVAA
                     DVGFPCIIRPSFTMGGSGGGIAYNREEFEEICARGLDLSPTKELLIDESLIGWKEYEM
                     EVVRDKNDNCIIVCSIENFDAMGIHTGDSITVAPAQTLTDKEYQIMRNASMAVLREIG
                     VETGGSNVQFAVNPKNGRLIVIEMNPRVSRSSALASKATGFPIAKVAAKLAVGYTLDE
                     LMNDITGGRTPASFEPSIDYVVTKIPRFNFEKFAGANDRLTTQMKSVGEVMAIGRTQQ
                     ESLQKALRGLEVGATGFDPKVSLDDPEALTKIRRELKDAGAERIWYIADAFRAGLSVD
                     GVFNLTNIDRWFLVQIEELVRLEEKVAEVGITGLNAEFLRQLKRKGFADARLAKLAGV
                     REAEIRKLRDQYDLHPVYKRVDTCAAEFATDTAYMYSTYEEECEANPSTDREKIMVLG
                     GGPNRIGQGIEFDYCCVHASLALREDGYETIMVNCNPETVSTDYDTSDRLYFEPVTLE
                     DVLEIVRIEKPKGVIVQYGGQTPLKLARALEAAGVPVIGTSPDAIDRAEDRERFQHAV
                     DRLKLKQPANATVTAIEMAVEKAKEIGYPLVVRPSYVLGGRAMEIVYDEADLRRYFQT
                     AVSVSNDAPVLLDHFLDDAVEVDVDAICDGEMVLIGGIMEHIEQAGVHSGDSACSLPA
                     YTLSQEIQDVMRQQVQKLAFELQVRGLMNVQFAVKNNEVYLIEVNPRAARTVPFVSKA
                     TGVPLAKVAARVMAGKSLAEQGVTKEVIPPYYSVKEVVLPFNKFPGVDPLLGPEMRST
                     GEVMGVGRTFAEAFAKAQLGSNSTMKKHGRALLSVREGDKERVVDLAAKLLKQGFELD
                     ATHGTAIVLGEAGINPRLVNKVHEGRPHIQDRIKNGEYTYIINTTSGRRAIEDSRVIR
                     RSALQYKVHYDTTLNGGFATAMALNADATEKVISVQEMHAQIK"
     gene            complement(56709..57857)
                     /gene="carA"
                     /locus_tag="OO357_000630"
     CDS             complement(56709..57857)
                     /gene="carA"
                     /locus_tag="OO357_000630"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HCR5885684.1"
                     /translation="MIKSALLVLEDGTQFHGRAIGATGSAVGEVVFNTSMTGYQEILT
                     DPSYSRQIVTLTYPHIGNVGTNDADEESSQVHAQGLVIRDLPLIASNFRNTEDLSSYL
                     KRHNIVAIADIDTRKLTRLLREKGAQNGCIIAGDNPDAALALEKARAFPGLNGMDLAK
                     EVTTAEAYSWTQGSWTLTGGLPEAKKEDELPFHVVAYDFGAKRNILRMLVDRGCRLTI
                     VPAQTSAEDVLKMNPDGIFLSNGPGDPAPCDYAITAIQKFLETDIPVFGICLGHQLLA
                     LASGAKTVKMKFGHHGGNHPVKDVEKNVVMITAQNHGFAVDEATLPANLRVTHKSLFD
                     GTLQGIHRIDKPAFSFQGHPEASPGPHDAAPLFDHFIKLIEQYRKTAK"
     gene            complement(58313..59134)
                     /gene="dapB"
                     /locus_tag="OO357_000631"
     CDS             complement(58313..59134)
                     /gene="dapB"
                     /locus_tag="OO357_000631"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HCR5885685.1"
                     /translation="MHDANIRVAIAGAGGRMGRQLIQAALALEGVQLGAALEREGSSL
                     LGSDAGELAGAGKTGVTVQSSLDAIKDDFDVFIDFTRPEGTLNHLAFCRQHGKGMVIG
                     TTGFDEAGKQAIRDAAADIAIVFAANFSVGVNVMLKLLEKAAKVMGDYTDIEIIEAHH
                     RHKVDAPSGTALAMGEAIAHALDKDLKDCAVYSREGHTGERVPGTIGFATVRAGDIVG
                     EHTAMFADIGERLEITHKASSRMTFANGAVRSALWLSGKESGLFDMRDVLDLNNL"
     gene            complement(59301..60215)
                     /gene="rihC"
                     /locus_tag="OO357_000632"
     CDS             complement(59301..60215)
                     /gene="rihC"
                     /locus_tag="OO357_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside hydrolase RihC"
                     /protein_id="HCR5885686.1"
                     /translation="MRLPIFLDTDPGIDDAVAIAAAIFAPELDLQLMTTVAGNVSVEK
                     TTRNALQLLHFWNAEIPLAQGAAVPLVRAPRDAASVHGESGMAGYDFVEHNRKPLGIP
                     AFLAIRDALMRAPEPVTLVAIGPLTNIALLLLQCPECKPYIRRLVIMGGSAGRGNCTP
                     NAEFNIAADPEAAACVFRSGIEIVMCGLDVTNQAILTPDYLATLPELNRTGKMLHALF
                     SHYRSGSMQSGLRMHDLCAIAWLVRPDLFTLKPCFVAVETQGEFTSGTTVVDIDGCLG
                     KPANVQVALDLNVKGFQQWVAEVLALVP"
     gene            complement(60319..61269)
                     /gene="ispH"
                     /locus_tag="OO357_000633"
                     /gene_synonym="lytB"
     CDS             complement(60319..61269)
                     /gene="ispH"
                     /locus_tag="OO357_000633"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HCR5885687.1"
                     /translation="MQILLANPRGFCAGVDRAISIVENALAIYGAPIYVRHEVVHNRY
                     VVDSLRERGAIFIEQISEVPDGAILIFSAHGVSQAVRNEAKSRDLTVFDATCPLVTKV
                     HMEVARASRRGEESILIGHAGHPEVEGTMGQYSNPEGGMYLVESPDDVWKLTVKNEEK
                     LSFMTQTTLSVDDTSDVIDALRKRFPKIVGPRKDDICYATTNRQEAVRALAEQAEVVL
                     VVGSKNSSNSNRLAELAQRMGKHAFLIDDAKDIQEEWVKEVKCVGVTAGASAPDILVQ
                     NVVARLQQLGGGEAIPLEGREENIVFEVPKELRVDIREVD"
     gene            complement(61271..61720)
                     /gene="fkpB"
                     /locus_tag="OO357_000634"
     CDS             complement(61271..61720)
                     /gene="fkpB"
                     /locus_tag="OO357_000634"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5885688.1"
                     /translation="MSESVQSNSAVLVHFTLKLDDGTTAESTRNNGKPALFRLGDASL
                     SEGLEQHLLGLKVGDKTTFSLEPDAAFGVPSPDLIQYFSRREFMDAGEPEIGAIMLFT
                     AMDGSEMPGVIREINGDSITVDFNHPLAGQTVHFDIEVLEIDPALEA"
     gene            complement(61807..62301)
                     /gene="lspA"
                     /locus_tag="OO357_000635"
     CDS             complement(61807..62301)
                     /gene="lspA"
                     /locus_tag="OO357_000635"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HCR5885689.1"
                     /translation="MSQSICSTGLRWLWLVVVVLIIDLGSKYLILQNFALGDTVPLFP
                     SLNLHYARNYGAAFSFLADSGGWQRWFFAGIAIGISVLLAVMMYRSKATQKLNNIAYA
                     LIIGGALGNLFDRLWHGFVVDMIDFYVGDWHFATFNLADTAICVGAALIVLEGFLPSK
                     AKKQ"
     gene            complement(62301..65117)
                     /gene="ileS"
                     /locus_tag="OO357_000636"
     CDS             complement(62301..65117)
                     /gene="ileS"
                     /locus_tag="OO357_000636"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HCR5885690.1"
                     /translation="MSDYKSTLNLPETGFPMRGDLAKREPGMLARWTDDDLYGIIRAA
                     KKGKKTFILHDGPPYANGSIHIGHSVNKILKDIIVKSKGLSGYDSPYVPGWDCHGLPI
                     ELKVEQEYGKPGEKFTAAEFRAKCREYAATQVDGQRKDFIRLGVLGDWSHPYLTMDFK
                     TEANIIRALGKIIGNGHLHKGAKPVHWCVDCRSALAEAEVEYYDKTSPSIDVAFQAVD
                     QDALKAKFGVSNVNGPISLVIWTTTPWTLPANRAISIAPDFDYALVQIDGQAVILAKD
                     LVESVMQRIGVTDYTILGTVKGAELELLRFTHPFMGFDVPAILGDHVTLDAGTGAVHT
                     APGHGPDDYVIGQKYGLETANPVGPDGTYLPGTYPTLDGVNVFKANDIVVALLQEKGA
                     LLHVEKMQHSYPCCWRHKTPIIFRATPQWFVSMDQKGLRAQSLKEIKGVQWLPDWGQA
                     RIESMVANRPDWCISRQRTWGVPMSLFVHKDTEELHPRTLELMEEVAKRVEVDGIQAW
                     WDLDAKEILGDEADQYVKVPDTLDVWFDSGSTHSSVVDVRPEFAGHAADMYLEGSDQH
                     RGWFMSSLMISTAMKGKAPYRQVLTHGFTVDGQGRKMSKSIGNTVSPQDVMNKLGADI
                     LRLWVASTDYTGEMAVSDEILKRAADSYRRIRNTARFLLANLNGFDPAKDMVKPEEMV
                     VLDRWAVGCAKAAQEDILKAYEAYDFHEVVQRLMRFCSVEMGSFYLDIIKDRQYTAKA
                     DSVARRSCQTALYHIAEALVRWMAPILSFTADEVWGYLPGEREKYVFTGEWYEGLFGL
                     ADSEAMNDAFWDELLKVRGEVNKVIEQARADKKVGGSLEAAVTLYAEPELAAKLTALG
                     DELRFVLLTSGATVADYNDAPADAQQSEVLKGLKVALSKAEGEKCPRCWHYTQDVGKV
                     AEHAEICGRCVSNVAGDGEKRKFA"
     gene            complement(65160..66101)
                     /gene="ribF"
                     /locus_tag="OO357_000637"
     CDS             complement(65160..66101)
                     /gene="ribF"
                     /locus_tag="OO357_000637"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HCR5885691.1"
                     /translation="MKLIRGIHNLSQAPQEGCVLTIGNFDGVHRGHRALLQGLQEEGR
                     KRNLPVMVMLFEPQPLELFATDKAPARLTRLREKLRYLAECGVDYVLCVRFDRRFAAL
                     TAQNFISDLLVKHLRVKFLAVGDDFRFGAGREGDFLLLQKAGMEYGFDITSTQTFCEG
                     GVRISSTAVRQALADDNLALAESLLGHPFAISGRVVHGDELGRTIGFPTANVPLRRQV
                     SPVKGVYAVEVLGLGEKPLPGVANIGTRPTVAGIRQQLEVHLLDVAMDLYGRHIQVVL
                     RKKIRNEQRFASLDELKAQIARDELTAREFFGLTKPA"
     gene            complement(66109..66327)
                     /locus_tag="OO357_000638"
     CDS             complement(66109..66327)
                     /locus_tag="OO357_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2575 family protein"
                     /protein_id="HCR5885692.1"
                     /translation="MCRHSLRSDGAGFYQLAGCEYSFSAIKIAAGGQFLPVICSMAMK
                     SHFFLISVLNRRLTLTAVQGILGRFSLF"
     gene            66430..66693
                     /gene="rpsT"
                     /locus_tag="OO357_000639"
     CDS             66430..66693
                     /gene="rpsT"
                     /locus_tag="OO357_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006117633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HCR5885693.1"
                     /translation="MANIKSAKKRAIQSEKARKHNASRRSMMRTFIKKVYAAIEAGDK
                     AAAQKAFNEMQPIVDRQAAKGLIHKNKAARHKANLTAQINKLA"
BASE COUNT        16260 a        18246 c        17253 g        15264 t
ORIGIN      
        1 atgtccagtc ccgtactcta cgcgccagag ggaaattcac ctggcgcgta tttttgttcg
       61 cggattagct acggctgagc acgccgcggc gatagagatt acgtttaatg cgcgttaaac
      121 caggttttgg cttacgcggt tcgtccaggc ttgccaggac aatctccagc acacgctctg
      181 cgacatcgcg gtgacgttga gccactgcca gcaccggaca ctgtaagaag tcgagcagtt
      241 cgttatcgcc aaaggtggca attgccaggt cagaaggcag tttgccgtcg cgacgcagcg
      301 tgacatccat cactccttgc aacaacgcaa acgacgttgt gaacagcgcc tgcggcatcg
      361 gatgcgtttc cagccatttt tcgaataact gggcagccgc ctcccgctca tagctgttgg
      421 catacaggaa atgcacttcg cgcggatcat ctttccaagc agtacggaaa ccttgttcac
      481 gcaggaagct gacagaaagc tccggcagcg cgccaagata aagcaccgtc tcggcgggaa
      541 acttacgtaa ctcttccgcc agcatttcgg catcatcctg atcggcacca accacgctgg
      601 tgaaatgttc acgatcgagg gcgcggtcca gcgcgacaat cgggaacggg tcgttagccc
      661 agcgttgata aaaaggatgc tcaggtggca acgacgtcga aacaataatg gcatcaacct
      721 gacgctgtaa aaggtgctca atgcagcgca tttcgttgtc tggctgatct tctgagcagg
      781 caatcagcag ttgataaccc cgttgccgcg cctggcgttc aagatagtta gcgatgcggg
      841 tatagctggt gttctccaga tcggggatca caagaccaat agaacgtgtg cgtccagcac
      901 gaagcccagc tgccacggcg ttcgggtggt aattgtgctc acgcaccaca gccatgactt
      961 tttcaacggt tttgtcgctc acacggtatt gcttcgcttt gccgttaata acatagcttg
     1021 cagtggtccg cgacactccc gccagccgag cgatttcatc cagtttcaca attgcccctt
     1081 gcgtaaaatg taaaaaccat agccattgta ctggtatggg ttaaattcat taacatctaa
     1141 gcgcagaaaa ttaactgcgg caactgcttt tattccaggt tcccactgat ttcgcaaaaa
     1201 aagcccaacg tgacatgttg ggctgcaaat tgcgcactga gatcattctc aacgcattat
     1261 tttatcgccg cgcgaaagtc cgaccacacc agagcgagca acttccacaa ttttcgccac
     1321 atcgcgaatc gatgctaaaa atgcatcaag cttatcgctg gtgcctgcta attgaacggt
     1381 ataaagcgag ggggtgacat cgataatttg cccacggaat atttccgtat tacgtttcac
     1441 ttcgtcacgc ccgtaaccgc tggcctgaat tttcaccagc atgatttccc gctcaacatg
     1501 cgcgccctgc cccaactcac tcacgcgcaa gacatcgacc agcttgtgta attgcttttc
     1561 gatctgctca agtacttttt catcgcccac ggtctggatg gtcatacgcg ataatgtcgg
     1621 atcgtcggtt ggcgcaacgg tcaggctttc aatgttgtag cctcgctggg aaaaaaggcc
     1681 aatcacgcgg gataacgcgc ctgattcatt ttcgagtaag actgataata tccggcgcat
     1741 aatcaggttc tctccgtttt gcttaaccac atttcatcca ttccgccccc gcgaatctgc
     1801 atcgggtaga cgtgctcact gccatcgacg gtaacatcaa caaacaccag gcgattattg
     1861 cgcacctgtt ccagcgcctc gctaagtttg ctttccagct catgcggatg agagatctgg
     1921 atcccgacgt gaccataggc ttccgccaga cggacgaaat cgggtagtga ttgcatataa
     1981 gattgtgaat gacggccgga atagatcatg tcctgccact gtttcaccat ccccagatag
     2041 cggttattga gattcaccac cagtacgggc aactcgtatt gcaacgcggt agacagttcc
     2101 tggatgttca tctgaatact gccgtcgcca gtgacgcaaa ccacggtttc ttctggcaac
     2161 gccattttga cgcccagtgc cgcaggtaaa ccaaaaccca tcgtgccgag accgccggaa
     2221 ttgatccagc gacgcggttt gtcgaatgga taataaagcg cagcaaacat ctggtgctgc
     2281 ccgacatcgg acgtcacgta agcgtcccca ttagtcaacc gccaaagagt ctcgatcacc
     2341 gcctgcggtt taatcttttc actgtgagtg tcatatttca ggcactgaag agcgcgccac
     2401 tgttcaatct gctgccacca gtcgcggatc tcatccagcg gttgatgggc ggattcttgc
     2461 gacaggagtt caagcatttg ttcgaggacc tggcgagcat cccccacaat cgggatatcc
     2521 gcagtcacgg ttttagaaat ggaagtagga tcaatatcga tatgcagaac agtggcattt
     2581 gggcagtact ttgccagatt gttcgtcgtt cggtcatcaa atcgtacccc gacggcgaaa
     2641 atcacatccg cgttatgcat cgtcatattg gcttcgtagg taccgtgcat tcccagcatg
     2701 cccagtgcct gacgatgcgt tgccggaaac gcccccattc ccatcaatga ggaaacaacg
     2761 ggcagattca acgcctccac cgtttctttc aactgctgat ggcagcccgc catgattgcc
     2821 ccaccgccta cgtagacaac cggttttttt gccgctacca gcgtttgcag agcacgctta
     2881 atttgccctt tatgtccggt agtagtggga ttgtaagaac gcatactgac cgactcaggg
     2941 cagacatagg gtaatttgtt cgccggatta agaatatctt tcggtaaatc aacgaccact
     3001 ggtcctgggc gaccacttgc cgccagccag aaagcctttt tcagcacctg cggaatgtct
     3061 tccgtttgct taaccagaaa actgtgttta accaccggtc gcgaaatccc caccatgtcg
     3121 cactcctgaa aggcatcgta acctatcaac gaggtcgcta cctgcccgga aaggacaact
     3181 aatggaatgg aatccatata agcggtggcg atgccggtaa tcgcattggt cgcccttgga
     3241 cccgacgtta ccagcacgac gccgacttcc ccggtcgcac gcgccaggcc atcggccata
     3301 tgcaccgccg cctgctcatg acgaactaat acatgatcaa taccacccac ggtatgcaat
     3361 gcatcgtaaa tatcaaggac tgcgcctccg ggataaccga atacttgttt aacgccctga
     3421 tcgataagcg atcggacgac catctcggct ccagacaaca tctccatggc ctgcctcact
     3481 gtttgacgga aaaaatgtgt aaaacacatt aaccgtccga tcttgcttaa gcaattggca
     3541 gccagagaca taaacgcagg gtaataagaa taaacaggag gaaaggtgaa aagaaacgat
     3601 aaaatcctcc attacgaagg attaaattga taataaatcg gctgaatccc acaacttaca
     3661 gcaatttctc tattttttta attaatccta aaatagagag tccacacatt cagaataaaa
     3721 ttctacattc aataaaaaat gcagaataaa ccagacattc atgtctggcc tattctgcat
     3781 tcagttagcg tttgcaaatt gagactaatt gttcttccat ccactgatgg cctttatcgc
     3841 gcccggcagc ttcatgccaa gagagataac aggttctgct gttttgtttt aacggcagcg
     3901 gtaatacctg taattctaag gattcagcga actcttcagc cagccaacgc ggcgcaatag
     3961 cgaccaaatg cgtttgcgac accacgctaa gtacgctcat cattgccatg ccctgatacg
     4021 cgatactggc ttgcttatct accgtgtcat accaaggttg actaaatgac gcgaaacgat
     4081 cgagcgaaac cgccgcatgt tgttcgttat aaacatcatg tttcagtaac gggcctttaa
     4141 ttgttggatg atttttgctg gctaccagca ccatttcatc tttaaataat ggcacgctgg
     4201 taaattcagg acgatggaag tcttcataac taatcacaaa ctccgtttcc tgataacgca
     4261 gctgatgttc agtgttctga tttaatgaag acttgaacat aacatgtata tttggcgcaa
     4321 tctgctcaat gtgattataa atctgcgagg tcagaatgct gtctaacggg ctgcaaacac
     4381 aaagatgaaa tacacgttca ctgctcgcgg gttcaaaacc tgaaccaggc aattcatttt
     4441 gtactagttg caatgcctgg cgaactgaac caaaaagttg aaatgcgcga gcagtcggtt
     4501 gaataccacg gccataacga acaaaaagct cgtcattaaa catcaccttc aggcgtgcaa
     4561 cagcgttact gaccgcaggt tgcgacatac ccagaacatg agcggcacgg gtaatgtttt
     4621 gctcctgcat cacggcatcg aaaacggtta ataagttgag atcgaccatg cgtagctgtg
     4681 gcttgcttaa ctccgccgtc tctgaatgat ctgtttgtac ctctggcata cttaactcca
     4741 ctgtcacact taactccctt tcccttattg gaatgcagag aaataatcct gaagaatatg
     4801 atttatcatg catataaaat aagaaaaagc aaaatgagta aaattcggaa aacataaaga
     4861 tacgggttct gataaaaaca gaatccattg ccgtggaatg agtcatttac cacagcataa
     4921 taatccataa tgttttgttg cgaaaacaat ctaatcataa ctaccgcgaa tactcaatca
     4981 tctacaaaat ggattaaatg tgcttattta ataattaatt tatatattta atgcattaat
     5041 tcttaacatt aattgttcaa taatattcac caaatcaata tcaaaaaaaa tcgcaaaaca
     5101 tataattcaa tacaaatcat caggataggt tttgcaacgc gtgcattttg tccccttttt
     5161 cctcgttgat tagatgcaaa aatttatgct gaaatatgtc aaccgatgaa aagcgtcggt
     5221 agttaagcag aaattaatat cgcttacttt aaccaccgta gcacaattag ctaattttac
     5281 ggatgcagaa ctcacgctgg cgggacgttt ttattgcgtc agggttgaca tccgtttttg
     5341 tatccagtaa ctctaaaagc atatcgcatt catctggagc tgatttaatg actcacatcg
     5401 ttcgctttat cggtctacta ctactaaacg catcttcttt gcgcggtaga cgagtgagcg
     5461 gcatccagca ttaagccagc acgcagtcaa acaaaaaacc cgcgccattg cgcgggtttt
     5521 tttatgcccg aagcgaggcg ctctaaaaga gacaaggacc caaaccatga gccagcaagt
     5581 cattattttc gataccacat tgcgcgacgg tgaacaggcg ttacaggcaa gcttgagtgt
     5641 gaaagaaaaa ctgcaaattg cgctggccct tgagcgtatg ggtgttgacg tgatggaagt
     5701 cggtttcccc gtctcttcgc cgggcgattt tgaatcggtg caaaccatcg cccgccaggt
     5761 taaaaacagc cgcgtatgtg cgttagctcg ctgcgtggaa aaagatatcg acgtggcggc
     5821 cgaatccctg aaagtcgccg aagccttccg tattcatacc tttattgcta cctccccaat
     5881 gcacatcgcc accaagctgc gcagcaccct agacgaggtg atcgaacgcg ctatctatat
     5941 ggtgaaacgc gcccgtaatt acaccgatga tgttgaattt tcttgcgaag atgccgggcg
     6001 tacacccatt gccgatctgg cgcgagtggt cgaagcggcg attaatgccg gtgccaccac
     6061 catcaacatt ccggacaccg tgggctacac catgccgttt gagttcgccg gaatcatcag
     6121 cggcctgtat gaacgcgtac ctaacatcga caaagccatt atttccgtac atacccacga
     6181 cgatttaggc ctggcggtcg gcaactcact ggcggcggta catgccggtg cacgccaggt
     6241 ggaaggtgca atgaacggga tcggcgagcg tgccggtaac tgttcgctgg aagaagtcat
     6301 catggcgatt aaagttcgta aggatattct caacgtccac accgccatta atcaccaaga
     6361 gatatggcgc accagccagt tagttagcca gatttgtaat atgccgatcc cggcaaacaa
     6421 agccattgtt ggcagcggcg cattcgctca ctcctccggt atccaccagg atggcgtgct
     6481 gaaaaaccgc gaaaactacg aaatcatgac accagaatct attggtctga accaaatcca
     6541 gctgaatctg acctctcgtt cggggcgtgc ggcggtgaaa catcgcatgg atgagatggg
     6601 gtataaagaa agtgaatata atttagacaa tttgtacgat gctttcctga agctggcgga
     6661 caaaaaaggt caggtgtttg attacgatct ggaggcgctg gccttcatcg gtaagcagca
     6721 agaagagccg gagcatttcc gtctggatta cttcagcgtg cagtctggct ctaacgatat
     6781 cgccaccgcc gccgtcaaac tggcctgtgg cgaagaagtc aaagcagaag ccgccaacgg
     6841 taacggtccg gtcgatgccg tctatcaggc aattaaccgc atcactgact ataacgtcga
     6901 actggtgaaa tacagcctga ccgccaaagg tcacggtaaa gatgcgctgg gtcaggtgga
     6961 tattgtcacc aactacaacg gtcgccgctt ccacggcgtc ggcctggcca ccgatattgt
     7021 cgagtcctcc gccaaagcca tggtgcacgt actgaacaat atctggcgtg ccgcagaagt
     7081 cgaaaaagag ttgcaacgca aaactcaaca caacgaaaac aacaaggaaa ccgtgtgatg
     7141 tcgaagaatt accatattgc cgtattgccg ggagacggta ttggtccgga agtgatgacc
     7201 caggcgctga aagtgctgaa tgccgtgcgc aaccgctttg cgatgcgcat caccaccagc
     7261 cattacgatg taggcggcgc agccattgat aaccacgggc aaccactgcc gcctgcgacg
     7321 gttgaaggtt gtgagcaagc cgatgccgtg ctgtttggct cggtaggcgg cccgaagtgg
     7381 gaacatttac caccagacca gcaaccagaa cgcggcgcgc tgctgccgct gcgtaagcac
     7441 ttcaaattat tcagcaacct gcgcccggca aaactgtatc aggggctgga agcattctgt
     7501 ccgctgcgtg cagacattgc cgctaacggc ttcgacatcc agtgcgtgcg cgaactgacc
     7561 ggcggcatct atttcggtca gccaaaaggc cgcgaaggta gcggacaata tgaaaaagcg
     7621 tttgataccg aggtgtatca ccgttttgag atcgagcgta ttgcccgcat cgcgtttgaa
     7681 tctgctcgta aacgtcgcca caaagtcacc tcaatcgaca aagctaacgt gctgcaatcc
     7741 tctattttat ggcgtgaaat cgtcaacgag atcgccacgg aatacccgga tgtcgaactg
     7801 gcgcatatgt acatcgacaa cgccaccatg cagctgatta aagatccatc acagtttgac
     7861 gttctgctgt gctccaacct gtttggcgac attctgtctg acgagtgcgc aatgatcact
     7921 ggctcgatgg gcatgttgcc ttccgccagc ctgaacgagc aaggttttgg actgtatgaa
     7981 ccggcgggcg gctcggcacc agatatcgca ggcaaaaaca tcgccaaccc gattgcacaa
     8041 atcctgtcgc tggcactgct gctgcgttac agcctggatg ccgatgatgc ggcttccgcc
     8101 attgaacgcg ccattaaccg tgcattagaa gaaggcattc gcaccgggga tttagcccgt
     8161 ggcgctgccg ccgttagtac cgatgaaatg ggcgatatca ttgcccgcta tgtggcagaa
     8221 ggggtgtaat catggctaaa acgttatacg aaaaattatt cgacgctcac gtagtgtacg
     8281 aagccgaaaa cgaaaccccg ctgttatata tcgaccgcca cctggtgcat gaagtgacct
     8341 caccgcaggc gttcgatggt ctgcgcgccc acggtcgccc ggtacgtcag ccgggcaaaa
     8401 ccttcgccac tatggatcac aacgtctcta cccagaccaa agacattaat gcctgtggtg
     8461 aaatggcgcg catccagatg caggagctga ttaaaaactg caaagaattt ggcgtcgagc
     8521 tgtatgacct gaatcacccg tatcagggga tcgtccacgt aatggggccg gaacagggcg
     8581 tcaccttgcc ggggatgacc attgtctgcg gcgactcgca taccgccacc cacggcgcgt
     8641 ttggcgcact ggcctttggt atcggcactt ccgaagttga acacgtactg gcaacacaaa
     8701 ccctgaaaca gggccgtgcg aagaccatga aaattgaagt ccagggcaaa gccgcgccgg
     8761 gcattaccgc aaaagatatc gtgctggcaa ttatcggtaa aactggcagc gcaggcggca
     8821 ccgggcatgt ggtggagttt tgcggcgaag caattcgtga tttaagcatg gaaggtcgta
     8881 tgaccctgtg caatatggcg atcgaaatgg gcgcaaaagc cgctctggtt gctcctgacg
     8941 aaaccacctt taactatgtc aaaggccgtc tgcatgcgcc gaaaggcaaa gatttcgacg
     9001 acgccgttgc ctactggaaa accctgcaaa ccgacgaagg cgcaactttc gataccgttg
     9061 tcactctgca agcagaagaa atttcaccgc aggtcacctg gggcaccaat cccggccagg
     9121 tgatttctgt gaacgacaat attcccgatc cggcttcgtt tgccgatccg gttgaacgtg
     9181 cgtcggcaga aaaagcgctg gcctatatgg ggctgaaacc gggtattccg ctgaccgaag
     9241 tggctatcga caaagtgttt atcggttcct gtaccaactc tcgcattgaa gatttacgcg
     9301 cggcagcgga gatcgccaaa gggcgaaaag tcgcgccagg cgtgcaggca ctggtggttc
     9361 ccggctctgg cccggtaaaa gcccaggcgg aagcggaagg tctggataaa atatttattg
     9421 aagccggttt tgaatggcgc ttgcctggct gctcaatgtg tctggcgatg aacaacgacc
     9481 gtctgaatcc gggcgaacgt tgtgcatcca ccagcaaccg taactttgaa ggccgccagg
     9541 ggcgcggcgg gcgcacgcat ctggtcagcc cggcaatggc tgccgctgct gctgtgaccg
     9601 gacatttcgc cgacattcgc aacattaaat aaggagcaca ccatggcaga gaaatttatc
     9661 aaacacacag gcctggtggt tccgctggat gccgccaatg tcgataccga tgcaatcatc
     9721 ccgaaacagt ttttgcagaa agtgacccgt acgggttttg gcgcgcatct gtttaacgac
     9781 tggcgttttc tcgatgaaaa aggccaacag ccaaacccgg acttcgtgct gaacttcccg
     9841 cagtatcagg gcgcttccat tttgctggca cgagaaaact tcggctgtgg ctcctcgcgt
     9901 gagcacgcgc cctgggcatt gaccgactac ggttttaaag tggtgattgc gccgagtttt
     9961 gctgacatct tctacggcaa tagctttaac aaccagctgc tgccggtgaa attaagcgat
    10021 gcagaagtgg acgaactgtt tgcgctggtg aaagccaatc cggggatcca tttcgacgtg
    10081 gatctggaag cgcaagaggt gaaagcggga gagaaaacct atcgctttac catcgatgcc
    10141 ttccgccgcc actgcatgat gaatggtctg gacagcatcg gcctgaccct gcagcacgac
    10201 gacgctattg cgtcttatga agcaaaacaa cctgcgttta tgaattagac cccttgcccg
    10261 gtcaaatgac cgggctttcc gctatcgtcc acgtcatcaa acgtctttaa cctttgcggt
    10321 taaaaataat gcgacaacag aaataatcgc aattacccag tagacagcaa agtgccccca
    10381 actttgtgca attgctccct gaataacacc agccagaatt accccggtag agatagtgtt
    10441 ggtaaataag gtggtagctg cccccgctct tccaggcatt aaatcctgaa accatagcat
    10501 cccaataccc gcaacaatgc cgataaatac agcgttaaaa agttgcagcg tcatcaacgc
    10561 catacggcta tgaaagaaaa tcaatccagt gtaaaacagc actcctgcca ccactgcaat
    10621 gaccatcatt cgccgcttac cataacgttt gacatagtag ccagccagaa tcattgctgg
    10681 tatttccagt ccagctgccg tccccatcag gaaacccgcc agtttgtctg gcaatcctaa
    10741 atcgctactg atccacaatg gcatatcaat aatgtacata gtgttgcagg tccacattaa
    10801 cgtcgaggcg acaaataaca tccgtacgtt actatcctgc cagtcacctt gcattgataa
    10861 agcattttcc gacggcagtt ctacccgcgc cacagacaga agcataaatg caatcaatac
    10921 cagactgagt gtgaatatcc cggcggcaat cgaaaacatc actgtaaagc cgtaattcaa
    10981 cgccagcata aaggccagcg gtggaccgat tacccatgcc agagaaagct gcgcacgcat
    11041 caccgagcta aacatcacca cttctcgcgc cgagttatcc gcgtattccc gcgccagagc
    11101 aaataactgt ggcattgccg tattggccag agatgccaga agcacaccac aggtgataag
    11161 cgtcagataa tgacgattaa atgcaaacag tagcgcattg ccaatagcca tcaaacagca
    11221 aaatataatc agttttcgcc gatcgccctg actgtcagaa cgttttgcca accagaggct
    11281 tacgccgatc ccagcaatag cattcaccgt ataaaagagt ccgatccaga aaggttgcgc
    11341 gccaacctca cgactcagaa ataagctcaa tgtaggagcc tgtagcgccc cggccacccc
    11401 catcataaaa gcgaccagca taaatgccgc gtaaacaccg ttcatacggc gagccatcgt
    11461 cattatccag atcatacgtt ccctttttag cgcggcgaga ataaaaaaaa ccagcaggta
    11521 taatctgctg gcgggtgatt ttacaccaat actcagtcac acatgatgca ggcaagtcaa
    11581 ctttcagaat tgcggtcatc ccactgcatc agtccttcaa gcatggttaa tcgttgtggg
    11641 acgcttaacc aacgcaacca gcacaacttc gctgtcgcgg taaaatagtg ctgataaaac
    11701 tgacgcatgg ggcacctcct tgcttcatcg ttaggaatta ttgacttaat atagggaaaa
    11761 taaaattgct gtcttttgcg caggagttcc ccttttatgc catctgctcg tctgcaacaa
    11821 cagttcatcc gcctgtggca atgctgcgag ggtaaatcgc aggacacaac gctcaacgaa
    11881 ctggcagcgt tattgagctg ctcgcgtcgt catatgcgta ccctgctcaa caccatgcag
    11941 gatcgcggct ggctgacgtg ggaagcggaa gtcgggcgcg gtaaacgctc gcgtctgaca
    12001 ttcctctata ccgggctggc gcttcagcaa cagcgggcgg aagacctgct ggagcaggat
    12061 cgtatcgacc aactagtgca gttggttggc gacaaagcga ctgtgcggca aatgctggtt
    12121 tctcatctgg gccgcagctt ccgccagggg cggcacatcc tgcgcgtgct ctactatcgt
    12181 ccgttgcgta atctgctacc tggcagcgca ttgcgccgtt ccgaaaccca tattgcccgg
    12241 caaatcttca gttcgctaac gcgcataaat gaggaaaatg gggaactgga agcagacatc
    12301 gcccaccact ggcagcaaat atcaccgctt cactggcgtt tctttttgcg tccaggagtc
    12361 cattttcatc atggtcgtga actggaaatg gacgacgtga tcgcctcttt aaaacgaatc
    12421 aatacgctgc cgctctattc gcatattacg gacattgtgt cgccgacgcc ctggacgctg
    12481 gatatccatc tcacgcagcc ggaccgctgg ttgccgttac tgctggggca agttccggcg
    12541 atgatcctgc cgtgcgaatg ggaaaccctc agtaactttg ccagccatcc tattggcacc
    12601 ggtccgtatg cggtgattcg caacagcacc aatcaactga aaattcaggc attcgatgac
    12661 ttcttcggtt accgggcatt aatcgacgaa gttaacgtct gggttctgcc ggaaattgcc
    12721 gacgagccag ccggtggtct gatgctaaaa ggtccacagg gcgagaaaaa agagattgaa
    12781 agccgcctgg aggaaggttg ctactattta ctgttcgaca gccgcaccca tcgcggggcg
    12841 aatcagcaag tcagggactg ggtaagctat gtgctttctc caactaatct ggtctatttc
    12901 gctgaggaac agtaccagca actgtggttc ccggcttatg gactgctccc ccgttggcac
    12961 catgcccgca ccataaagag cgaaaaaccg gctggcctgg aaagcctcac cctgaccttt
    13021 tatcaggatc acagtgagca tcgggtgatt gccgggatca tgcagcagat tctggcaagt
    13081 caccaggtca cgctggaaat caaagagatc agctacgatc agtggcatga aggagagatc
    13141 gagagcgata tctggcttaa tagcgccaac tttacgctgc cgctggattt ttcgctgttc
    13201 gcgcacttgt gcgaggtgcc gctgctccaa cactgtattc cgatcgactg gcaagccgac
    13261 gccgcccgct ggcgcaatgg cgaaatgaac ctggcgaact ggtgccagca actggtcgcc
    13321 agtaaagcaa tggtgccgct tatccaccac tggctgatca ttcagggaca acgcagtatg
    13381 cgcggcctgc gtatgaatac cctcggctgg ttcgatttta aatcagcgtg gtttgcgcca
    13441 ccggatccat gattgctggt agataacaaa atcactacac taacgccgtt ctcaacgggg
    13501 tgccacgcaa acgcgtgcgc tgagaaaata cccgtcgaac ctgatccgga taacgccggc
    13561 gaagggattt gaggctcctt ctcaagtcct ttgccactct tttttgaggt gcaaagtgtt
    13621 aaaaaaatgt cttcccctgc tgttgctgtg cacagcgccc gttttcgcta aacccgttct
    13681 gactgtttat acctacgatt ccttcgccgc cgactggggg cctggtccaa agatcaaaaa
    13741 agcttttgaa gccaactgta actgcgaact gaaactggtg gcgctggaag atggcgtttc
    13801 gcttctcaac cgtctacgga tggaaggcaa aaatagtaaa gccgatgtgg tgctggggct
    13861 ggataacaat ctgttagacg ccgccagtaa aaccggacta tttgccaaaa gcggtgtggc
    13921 agcggatgcc gttaacgttc ccggcggctg gaataatgac actttcgtcc cgtttgatta
    13981 cggctatttc gccttcgtct atgacaagaa caaactgaaa aacccgccgc aaagcctgaa
    14041 agaattggtt gagagcgatc agaactggcg ggtgatttat caggatccgc gtaccagcac
    14101 gccggggctg ggtctgctgt tgtggatgca aaaagtctat ggcgatgacg ccccacaagc
    14161 ctggcaaaaa ctggcgaaga aaaccgtcac ggtcaccaaa ggctggagcg aagcctacgg
    14221 cctgttttta aaaggtgaaa gcgatctggt actgagttac accacctctc cggcttatca
    14281 cattctcgaa gagaaaaaag ataactacgc cgccgcgaac tttagcgaag gtcactatct
    14341 gcaggtggaa gtcgccgccc gcaccgctgc cagcaagcag ccggagctgg cgcaaaaatt
    14401 cctccagttt atggtttctc cggcttttca gaatgcgatc ccaaccggta actggatgta
    14461 tccggtggca aatgtcacgc tgcctgccgg gtttgaacaa ttgaccaaac cggccaccac
    14521 gctggagttc acaccagctg aagtggcggc acaacgtcag gcatggatta gcgaatggca
    14581 acgcgccgtc agccgttaat tcccggctgg ttaattccag gtgtaagcgc cgccacgctg
    14641 gtggtagcgg ttgcgctcgc ggcgtttctc gccctgtggt ggaacgcgcc gcagggtaac
    14701 tgggtggcag tctggcagga cagctacctg tggcatgtgg tgcgcttctc cttctggcag
    14761 gcgtttctct cggcgctact ctctgtcgta cccgcgatat tcctcgcccg cgccctctat
    14821 cgcaggcgct ttccgggtcg gctggcgctg ttgcgtctgt gcgcaatgac cttgatcctc
    14881 ccggtgctag ttgctgtttt cggcattctt agcgtctatg gtcgtcaggg ctggctggca
    14941 tcgctctgcc aatcgctcgg tctggagtgg accttttcgc cctacggcct gcaaggcatt
    15001 ttgctggcgc acgtattttt taatctgccg atggcgagcc gcttattact ccaggcactg
    15061 gaaaacattc ccggcgaaca acgtcagctt gccgcccagc ttgggatgcg cggctggcat
    15121 tttttccgct tcgtcgaatg gccgtggcta cggcgacaaa tcccgccggt tgccgcgctt
    15181 atctttatgc tctgtttcgc cagcttcgcc accgtgctat cgctgggcgg cggtccgcag
    15241 gcaaccacta tcgagctggc tatttatcag gcgctgagtt acgactacga tcctgcccgc
    15301 gcggcgatgc tggcgctgct ccagatggtg tgttgccttg ggctggtgct gttgagtcag
    15361 cgactaagta aggccattgc gcccggcacc acgctgctgc aaggctggcg cgatccggac
    15421 gatcgtctgc atagccgcat ttgcgacacg gtgttaatag tgctggcgct gctgctgttg
    15481 ctgccaccgt tgctggcggt gatcgtcgat ggggtaaatc gccagttgcc ggaagtgctg
    15541 gcacaaccgg tgctgtggca ggcgctgtgg acctcgttgc gtattgcgct ggcggcaggt
    15601 gtattgtgcg tagtgctgac catgatgctg ctatggagca gtcgcgaact gcgggcgcgg
    15661 cagaaaatgc tggcgggtca ggcgctggag atgagcggca tgttgatcct cgccatgccg
    15721 gggattgtgc tggctaccgg cttcttttta ctgctcaaca acactatcgg cctgccacaa
    15781 tctgctgacg gcattgtgat tttcaccaat gcgttaatgg cgatccctta tgcgctgaaa
    15841 gtactggaaa acccgatgcg cgatatcacc gcccgctaca gtatgttgtg tcagtcgctg
    15901 ggcattgaag gctggtcgcg cttaaaagtg gtcgagctgc gcgccctgaa acgtccactg
    15961 gcgcaggcgc tggcttttgc ctgcgtgctg tcgattggtg attttggcgt agtggcgttg
    16021 ttcggtaacg atgatttccg taccctgccg ttttatctct accagcaaat tggctcctat
    16081 cgcagccagg acggcgcggt caccgcgtta attctgctac tactctgttt tctgctgttt
    16141 accgtgattg aaaaactacc ggggcgaaat gttaaaactg actgatatca cctggcttta
    16201 tcaccatttg ccgatgcgtt ttagcttaac ggtagaacgc ggcgagcagg tggcaatcct
    16261 cgggccaagc ggcgcgggta aaagtaccct gctgaatttg attgccggtt ttctgacgcc
    16321 agccagcggt tcgctgacta tcgatggtgt tgatcacacc acgacgccgc cgtcacgccg
    16381 tccggtgtcg atgctgtttc aggagaacaa cctgttcagc catctgacgg tcgcgcagaa
    16441 catcgggctg gggctaaatc cggggttgaa actgaacgcg gcacagcagg agaaaatgca
    16501 cgcgattgcc cgtcagatgg ggattgataa tttaatggcg cggttaccgg gcgagctttc
    16561 cggcggtcag cgacagcgcg tggcgttagc gcgttgtctg gtgcgcgaac agccgatttt
    16621 attgctcgat gaaccgttct ctgcgctcga tccggcgtta cgtcaggaga tgctgacgct
    16681 ggtgagcacg agctgccagc agcaaaaaat gacgctattg atggtgtcgc acagtgtgga
    16741 agatgcggcg cggatcgcca cacgctcggt ggtggtcgcc gacgggcgca tcgcctggca
    16801 gggtaagact gaagaattgt tgagcggtaa ggcgagtgct tcggcaatat tggggattac
    16861 gggttagtat gcactgtcag atgcggcgtg aacaccttat ccgacctacg gacagtgcgt
    16921 tttgtaggcc tgataagacg cggtcagcgt cgcatgaggc aacccgcaca agacaatccc
    16981 ctaaacccca accactttac gcaaaatatc gatatacacc ggcatcaacg ggtggcgaat
    17041 taacaccacc agcgccacca cgccgatggc agaaatcaac ggcgtcagcc acaacaaacg
    17101 gccgcgcgac aaataatgac ttaaacgatc ggttgcttta ccgctgcgcc ataaccgcca
    17161 gcacagccag ccaccaaccc acaaaaatac cgccgtcgcc agcagcaacc atttaaactc
    17221 gccgctctgc attccggctg gaatatcgat cgccgcgccc gccagaatcc caggcaggaa
    17281 gtaaaacggt ggccacagca ggcagccgat aatattcggc gtaatgaatt ttgccaccgg
    17341 cagatccagc attcccgcca ccattggcac cagcggacgc gtcgggccaa caaagcgacc
    17401 gaccagaatg gtgaacatgc tgtgttgatg caacgcatgt tcagttttat cgagtaatgc
    17461 tttgtttttc ttcagaaatg accagcgatg caacggcttt ttaaaacgcc agcccagcca
    17521 gaaggagatc cagtcgccca gcagacaacc gacaatccct gccagccaag cgtgccagaa
    17581 acttaactcg ccgctgccaa tcagcgctcc cagccccgcc atcagcaccg taccgggtag
    17641 aatcaaaccg accagcgcca gcgactccag aaaggccacc aacaccaccg ccatcaatga
    17701 atacacggta gattgggtaa taaagtgttc cagcaatgct tgcataatgt gcctgtcaaa
    17761 tggacgaagc agggattctg caaaccctat gctactccgt caagccgtca attgtctgat
    17821 tcgttaccaa ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac
    17881 ggaactcgct cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat
    17941 cgtcaaaacc aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca
    18001 gcttcgcctg gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct
    18061 ggcggaaaag atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga
    18121 tatcaaaatt gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacacgat
    18181 tatccatcgg tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct
    18241 caagcagatt catcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga
    18301 tttgcccaaa caggtcgctg aaatacggct ggtgcgcttc atccgggcga aagaaacccg
    18361 tattggcaaa tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt
    18421 aaacccactg gtgataccat tcgcgagcct ccggatgaca accgtagtga tgaatctctc
    18481 ctggcgggaa cagcaaaata tcacccggtc ggcagacaaa ttctcgtccc tgatttttca
    18541 ccaccccctg accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt
    18601 cgataaaaaa atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg
    18661 cattaaacga gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac
    18721 tcccgccatt cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg
    18781 tcttttactg gctcttctcg ctaacccaac cggtaacccc gcttattaaa agcattctgt
    18841 aacaaagcag gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca
    18901 gaaaagtcca cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat
    18961 ccataagatt agcggatcct acctgacgct ttttatcgca actctctact gtttctccat
    19021 acccgttttt ttggatggag tgaaacgatg gcgattgcaa ttggcctcga ttttggcagt
    19081 gattctgtgc gagctttggc ggtggactgc gccagcggtg aagagatcgc caccagcgta
    19141 gagtggtatc cccgttggca aaaagggcaa ttttgtgatg ccccgaataa ccagttccgt
    19201 catcatccgc gtgactacat tgagtcaatg gaagcggcac tgaaaaccgt gcttgcagag
    19261 cttagcgtcg aacagcgcgc agctgtggtc gggattggcg ttgacagtac cggctcgacg
    19321 cccgcaccga ttgatgccga cggtaacgtg ctggcgctgc gcccggagtt tgccgaaaac
    19381 ccgaacgcga tgttcgtatt gtggaaagac cacactgcgg ttgaagaagc ggaagagatt
    19441 acccgtttgt gccacgcgcc gggcaacgtt gactactccc gctatattgg cggtatttat
    19501 tccagcgaat ggttctgggc aaaaatcctg catgtgactc gccaggacag cgccgtggcg
    19561 caatctgccg catcgtggat tgagctgtgc gactgggtgc cagctctgct ttccggtacc
    19621 acccgcccgc aggatattcg tcgcggacgt tgcagcgccg ggcataaatc tctgtggcac
    19681 gaaagctggg gcggcttgcc gccagccagt ttctttgatg agctggaccc gatcctcaat
    19741 cgccatttgc cttccccgct gttcactgac acctggactg ccgatattcc ggtgggcacc
    19801 ttatgcccgg aatgggcgca gcgtctcggc ctgcctgaaa gcgtggtgat ttccggcggc
    19861 gcgtttgact gccatatggg cgcagttggc gcaggcgcac agcctaacgc actggtaaaa
    19921 gttatcggta cttccacctg cgacattctg attgccgaca aacagagcgt tggcgagcgg
    19981 gcagttaaag gtatttgcgg tcaggttgat ggcagcgtgg tgcctggatt tatcggtctg
    20041 gaagcaggcc aatcggcgtt tggtgatatc tacgcctggt ttggtcgcgt actcggctgg
    20101 ccgctggaac agcttgccgc ccagcatccg gaactgaaag cgcaaatcaa cgccagccag
    20161 aaacaactgc ttccggcgct gaccgaagca tgggccaaaa atccgtctct ggatcacctg
    20221 ccggtggtgc tcgactggtt taacggtcgt cgcacgccaa acgctaacca acgcctgaaa
    20281 ggggtgatta ccgatcttaa cctcgctacc gacactccgc tgctgttcgg cggtttgatt
    20341 gctgccaccg cctttggcgc acgcgcaatc atggagtgct ttaccgatca ggggatcgcc
    20401 gtcaataacg tgatggcgct gggcggcatc gcgcggaaaa accaggtcat tatgcaggcc
    20461 tgctgcgacg tgctgaatcg cccgctgcaa attgttgcct ctgaccagtg ctgtgcgctc
    20521 ggtgcggcga tttttgctgc cgtcgccgcg aaagtgcacg cagacatccc atcagcccag
    20581 caaaaaatgg ccagtgcggt agagaaaacc ctgcaaccgc gcagcgaaca ggcacaacgc
    20641 tttgaacagc tttatcgccg ctatcagcaa tgggcgatga gcgccgaaca acactatctt
    20701 ccaacttccg ccccggcaca ggctgcccag gccgttgcga ctctataagg acacgataat
    20761 ggcgattttt gataattatg aagtgtggtt tgtcattggc agccagcatc tgtatggccc
    20821 ggaaaccctg cgtcaggtca cccaacatgc cgagcacgtt gttaatgcgc tgaatacgga
    20881 agcgaaactg ccctacaaac tggtgttgaa accgctgggc accacgccgg atgaaatcac
    20941 cgctatttgc cgcgacgcga attacgacga tcgttgcgct ggtctggtgg tgtggctgca
    21001 caccttctcc ccggccaaaa tgtggatcaa cggcctgacc atgctcaaca aaccgttgct
    21061 gcaattccac acccagttca acgcggcgct gccgtgggac agtatcgata tggactttat
    21121 gaacctgaac cagactgcac atggcggtcg cgagttcggc ttcattggcg cgcgtatgcg
    21181 tcagcaacat gccgtcgtta ccggtcactg gcaggataaa caagcccatg agcgtatcgg
    21241 ctcctggatg cgtcaggcgg tctctaaaca ggatacccgt catctgaaag tctgccgatt
    21301 tggcgataac atgcgtgaag tggcggtcac cgatggcgat aaagttgccg cacagatcaa
    21361 gttcggtttc tccgtcaata cctgggcggt tggctatctg gtgcaggtgg tgaactccat
    21421 cagcgacggc gatgttaacg cgctggtcga tgagtacgaa agctgctaca ccatgacgcc
    21481 tgccacacaa atccacggcg aaaaacgaca gaacgtgctg gaagcggcgc gtattgagct
    21541 ggggatgaag cgtttcctgg aacaaggtgg cttccacgcg ttcaccacca cctttgaaga
    21601 tttgcacggt ctgaaacagc ttccaggtct gcccgtacag cgtctgatgc agcagggtta
    21661 cggctttgcg ggcgaaggcg actggaaaac cgccgccctg cttcgcatca tgaaggtgat
    21721 gtcaaccggt ctgcagggcg gcacctcctt tatggaggac tacacctatc acttcgagaa
    21781 aggtaatgac ctggtgctcg gctcccatat gctggaagtc tgcccgtcga ttgccgtaga
    21841 agagaaaccg atcctcgacg ttcagcatct cggtattggt ggtaaggacg atcctgcccg
    21901 actgatcttc aatacccaaa ccggtccagc gattgtcgcc agcctgattg atctcggcga
    21961 tcgttaccgt ctgctggtta actgtatcga cacggtgaaa acaccgcact ccctgccgaa
    22021 actgccggtg gcgaatgcac tgtggaaagc gcaaccggat ctgccaactg cttccgaagc
    22081 gtggatcctc gctggtggcg cgcaccatac cgtcttcagc catgcgctga acctcaacga
    22141 tatgcgccag ttcgccgaga tgcacgacat tgaaatcacg gtgattgata acgacacccg
    22201 cctgccagcg tttaaagacg cactgcgctg gaacgaagtg tattacggat ttcgtcgcta
    22261 agtagccaca tcaggtatgt aacgcctgat gcgacgctga cgcgtcttat ctggcctaca
    22321 cgctgcgatt ttgtaggccg gataagcaaa gcgcatccgg cattcaacgc ctgatgcgac
    22381 gctggcgcgt cttatcaggc ctacgcgctg cgattttgta gaccggataa gcgaagcgca
    22441 tccggcacga aggagtcaac atgttaaaag atctcaaacg ccaggtatta gaagccaacc
    22501 tggcgctgcc aaaacacaac ctggtcacgc tcacatgggg caacgtcagc gccgttgatc
    22561 gcgagcgcgg cgtctttgtg atcaaacctt ccggcgtcga ttacagcgtc atgaccgctg
    22621 acgatatggt cgtggttagc atcgaaaccg gtgaagtggt tgaaggtacg aaaaagccct
    22681 cctccgacac gccaactcac cggctgctct atcaggcatt cccctccatt ggcggcattg
    22741 tgcatacgca ctcgcgccac gccaccatct gggcgcaggc gggtcagtcg attccagcaa
    22801 ccggcaccac ccacgccgac tatttctacg gcaccattcc ctgcacccgc aaaatgaccg
    22861 acgcagaaat caacggcgaa tatgagtggg aaaccggtaa cgtcatcgta gaaacctttg
    22921 aaaaacaggg tatcgatgca gcgcaaatgc ccggcgttct ggtccattcc cacggcccgt
    22981 ttgcatgggg caaaaatgcc gaagatgcgg tgcataacgc catcgtgctg gaagaggtcg
    23041 cttatatggg gatattctgc cgtcagttag cgccgcagtt accggatatg cagcaaacgc
    23101 tgctggataa acactatctg cgtaagcatg gcgcgaaggc atattacggg cagtaatgac
    23161 tgtataaaac cacagccaat caaacgaaac caggctatac tcaagcctgg ttttttgatg
    23221 gattttcagc gtggcgcagg caggttttat cttaacccga cactggcggg acaccccgca
    23281 agggacagaa gtctccttct ggctggcgac ggacaacggg ccgttgcagg ttacgcttgc
    23341 accgcaagag tccgtggcgt ttattcacgc cgatcaggtt ccccgcgctc agcatatttt
    23401 gcagggtgaa caaggctttc gtctgacacc gctggcgtta aaggattttc accgccagcc
    23461 ggtgtatggc ctttactgtc gcgcccatcg ccaattgatg aattacgaaa agcgtctgcg
    23521 tgaaggtggc gttaccgtct acgaggccga tgtgcgcccg ccagaacgct atctgatgga
    23581 gcggtttatc acctcaccgg tgtgggtcga gggtgatatg cacaatggca ctatcgttaa
    23641 tgcccgtctg aaaccgcatc ccgactatcg tccgccgctc aagtgggttt ctatagatat
    23701 tgaaaccacc cgccacggtg agctgtactg catcggcctg gaaggctgcg ggcagcgcat
    23761 cgtttatatg ctggggccgg agaatggcga cgcctcctcg cttgatttcg aactggaata
    23821 cgtcgccagc cgcccgctgc tgctggaaaa actcaacgcc tggtttgcaa accacgatcc
    23881 cgatgtgatc atcggctgga acgtggtgca gttcgatctg cgaatgctgc aaaaacatgc
    23941 cgagcgttac cgtcttccgc tgcgtcttgg gcgcgataat agcgagctgg agtggcgcga
    24001 gcacggcttt aaaaacggcg tcttttttgc ccaggctaaa ggtcggctaa ttatcgacgg
    24061 tatcgaggcg ctgaaatccg cgttctggaa tttctcttca ttctcgctgg aaactgtcgc
    24121 tcaggagcta ttaggcgaag gaaaatctat cgataacccg tgggatcgaa tggacgaaat
    24181 tgaccgccgt ttcgccgaag ataaacctgc gctggcaact tataacctga aagattgcga
    24241 gctggtgacg cagatcttcc acaaaactga aatcatgcca tttttactcg aacgagcaac
    24301 ggtgaacggc ctgccggtgg atcggcacgg cggttcggtg gcagcgtttg gtcatctcta
    24361 ttttccgcga atgcatcgcg ctggttatgt cgcgcctaat ctcggcgaag tgccgccgca
    24421 cgccagccct ggcggctacg tgatggattc acggccaggg ctttatgatt cagtgctggt
    24481 gctggactat aaaagcctgt acccgtcgat catccgcacc tttctgattg atcccgtcgg
    24541 gctggttgaa ggcatggcgc aacctgatcc agagcacagt accgaaggtt ttctcgatgc
    24601 ctggttctca cgggagaaac attgcctgcc ggagattgtg actaacatct ggcacgggcg
    24661 cgataaagcc aaacgtcagg gtaataagcc gctttcgcag gcgctgaaga tcattatgaa
    24721 tgccttttat ggcgtgctcg gcaccaccgc ctgccgcttc ttcgatccgc gcctggcatc
    24781 gtcgatcacc atgcgtggtc atcagatcat gcggcagacc aaagcattga ttgaagcgca
    24841 gggctacgac attatctacg gagataccga ctcgactttt gtctggctaa aaggtgcaca
    24901 ttcggaagaa gaggcggcga aaatcggtcg tgcactggtg cagcacgtta acgcctggtg
    24961 ggcggaaacg ctgcaaaaac aacggctgac cagcgcatta gaactggagt atgaaactca
    25021 tttctgccgt tttcttatgc caaccattcg cggagctgat accggcagta aaaagcgcta
    25081 cgccgggctg attcaggagg gcgacaagca gcggatggtg tttaaagggc tggaaaccgt
    25141 gcgcaccgac tggacgccgc tggcccagca gtttcagcag gagctgtacc tgcgtatctt
    25201 ccgcaacgag ccatatcagg agtacgtccg ccaaaccatc gacaaactga tggcgggtga
    25261 actggatgcg cggctggttt accgtaaacg ccttcgccgt ccgctgagcg aatatcagcg
    25321 taatgttccg cctcatgtac gcgccgctcg ccttgccgat gaagaaaatc aaaagcgcgg
    25381 tcgccccttg caatatcaga atcgcggcac cattaagtac gtatggacca ccaacggccc
    25441 ggagccgctg gactaccaac gttcaccact ggattacgaa cactatctga cccgccagct
    25501 acaacccgtg gcggagggaa tactcccttt tattgaggat aattttgcta cacttatgac
    25561 cgggcaactt gggctatttt gagcaaaaaa aagagttcgc cagataccat tttgatgcgt
    25621 gacgaatgct ttgccatcca gtaccatagc gccctttcca ttcctggacc tgaataacac
    25681 cactacctca taagcacggt agcgggtggt tattgcctgc aattaaagat atagagccga
    25741 acacatatgc cttttacact tggtcaacgc tggatcagcg atacagaaag cgaattggga
    25801 cttggaaccg ttgtcgcggt ggatgcgcga actgtcactt tacttttccc atctactggt
    25861 gaaaaccgtc tgtacgcacg cagtgattcc cccgtgaccc gcgtgatgtt caaccctggt
    25921 gataccatta ccagccatga cggctggcag atgcaagtcg aagaagtaaa agaagaaaat
    25981 ggcttgctga cctatatcgg tactcgcctg gatactgaag agtccggcgt agccctgcgt
    26041 gaagttttcc ttgatagcaa actggtgttc agcaaaccgc aggaccgtct gtttgccggg
    26101 cagattgacc gtatggaccg ctttgcgctg cgttatcgcg cgcgtaagta ttccagcgaa
    26161 cagttccgta tgccgtacag cggcctgcgc ggtcagcgta ccagcctgat cccgcatcag
    26221 ctcaacatcg ctcatgatgt tggtcgccgc cacgcgccgc gcgtcctgct ggctgacgaa
    26281 gtgggtttag ggaaaaccat tgaagccggg atgatcctgc atcagcaact gctctctggc
    26341 gctgctgaac gtgtgctgat tatcgtcccg gaaaccttac agcatcagtg gctggtagaa
    26401 atgctgcgcc gtttcaacct gcgctttgct ctgtttgatg atgagcgtta tgccgaagct
    26461 cagcacgatg cttacaaccc gtttgacacc gaacagctgg tgatttgctc gctggatttt
    26521 gcccgtcgta gcaaacagcg cttggaacat ctctgtgaag ccgaatggga cctgctggtg
    26581 gtcgatgaag cgcatcacct ggtgtggagc gaagatgcgc caagccgtga atatcaggcc
    26641 attgaacaac tggcagagca cgtgccgggc gttctgctgc tgaccgcgac cccggaacag
    26701 ctggggatgg aaagccactt cgcccgtctg cgtctgctgg acccgaaccg tttccacgat
    26761 tttgcgcagt tcgttgaaga gcagaaaaat tatcgtccgg ttgcggacgc cgttgccatg
    26821 ctgctggcag gtaacaaact gagcaatgac gaactgaaca tgctcggcga gatgatcggc
    26881 gagcaggata tcgagccgct gttgcaggca gcaaacagcg acagcgaaga tgcccagagc
    26941 gcccgtcagg agctggtttc gatgctgatg gatcgccacg gcaccagccg cgtgctgttc
    27001 cgtaacacgc gtaacggtgt gaaaggattc ccgaaacgcg agctgcacac cattaagctg
    27061 ccgctaccga cgcagtatca gacggctatt aaagtctccg gcattatggg cgcacgtaaa
    27121 agtgcggaag atcgtgctcg cgatatgctc tacccggagc gtatttatca ggaatttgaa
    27181 ggtgataacg ccacctggtg gaacttcgat ccgcgcgttg agtggctgat gggctacctg
    27241 accagccatc gctctcagaa agtgctggtg atctgcgcca aagctgccac tgcgctgcaa
    27301 ctggagcagg tactgcgcga acgtgaaggt attcgcgctg cggtgttcca cgaaggtatg
    27361 tcgattatcg aacgtgaccg cgctgccgcc tggtttgccg aagaagacac cggcgcacag
    27421 gtactgctgt gctcagaaat cggttctgaa ggacgtaact ttcagttcgc cagtcacatg
    27481 gtgatgtttg acctgccatt caacccggat ctactggagc agcgtattgg tcgtctggat
    27541 cgtatcggcc aggcgcacga tattcagatc catgtgcctt atctggagaa aaccgctcag
    27601 tcggtgctgg tgcgctggta tcacgaaggt ctggatgcat ttgagcacac ctgcccgacc
    27661 ggacgcacta tttacgatag cgtatacaac gatctgatta actatctggc ttcaccggat
    27721 caaaccgaag gctttgacga tctgatcaaa agctgccgcg agcaacatga agcgctgaaa
    27781 gcacagctgg aacagggtcg tgaccgcctg ctggaaatcc actccaacgg tggcgaaaaa
    27841 gcccaggcac tggcagaaag cattgaagag caggatgacg ataccaacct gatcgccttc
    27901 gccatgaacc tgttcgatat tatcggtatc aatcaggacg atcgcggcga caacatgatc
    27961 gtgctgacgc cgtccgatca tatgctggtg ccggacttcc ctggcctgtc ggaagatggc
    28021 atcaccatca cctttgatcg tgaagtggcg ctggcgcgtg aagatgcaca gtttattacc
    28081 tgggagcatc cgctgatccg caacggtctg gatctgatcc tttctggcga taccggtagc
    28141 agcacgattt cactgttaaa aaacaaagcg ttgccggtag gtacgctgtt ggtggaactg
    28201 atttatgtgg ttgaagccca ggctccgaag cagttgcagc tcaaccgctt cctgccaccg
    28261 acgccggtac gtatgctgct ggataaaaac ggcaacaacc tggcggcgca ggtagagttt
    28321 gaaaccttta accgccagct taacgcggtt aaccgtcaca ccggcagcaa actggttaac
    28381 gccgtgcagc aggatgttca cgctatcctt caactgggtg aagcgcagat cgagaaatct
    28441 gcccgtgcat tgattgatgc agcgcgtaac gaagccgacg aaaaactgtc tgccgagctg
    28501 tctcgtctgg aagctctgcg tgcagtgaac ccgaacattc gtgacgacga actgaccgcc
    28561 attgagagca accgtcagca ggtaatggaa agcctggatc aggcaggttg gcgtctggat
    28621 gccctgcgtt tgatcgttgt aacgcatcag taacggagcc gaaaatgggg atggaaaact
    28681 acaatccacc gcaggaaccc tggttggtta tcctgtatca ggatgaccat attatggtgg
    28741 tcaacaagcc gagcggtttg ttgtcagtgc cgggtcgtct ggaagagcac aaagacagcg
    28801 tgatgacgcg cattcagcgt gattatccgc aggcagaatc ggtgcatcgt ctggatatgg
    28861 ctaccagcgg cgtgattgta gtggcgctga ccaaagccgc ggagcgggag ttaaaacgcc
    28921 agttccgcga gcgcgagccg aaaaagcagt atgtggcccg cgtctggggg catccatccc
    28981 ccgcagaagg tctggtggat ctgccgctga tttgcgactg gccaaaccgc ccgaaacaga
    29041 aagtctgtta cgaaacgggt aaacctgcgc agacggaata tgaagtggtg gagtatgcgg
    29101 cggataacac ggcaagagtg gtgttaaaac cgattaccgg gcgttcgcat caattgcgtg
    29161 tgcatatgct ggcgctgggg catccgattc tcggcgatcg tttttatgca tcaccagaag
    29221 cgagagcgat ggcaccgcgt ttgttgctac atgcagagat gttgacgatt acccatccgg
    29281 cgtatggcaa tagtatgacg tttaaagcgc cagcggattt ttaatcgtca cgctggcgca
    29341 gttcggtagg cggataaggc gttcacgccg catccggcaa ttgtgcgccg attgcctgat
    29401 gcgacgcggg ccgcgtctta tcaggcctac agtttaaaac ttatttaaac cctttctgct
    29461 gctttatcag ctcatatgcc tgctgaattt cctgcgcttt ctgcttcgcc atctccatca
    29521 tctcaggcgg caaacctttc gccaccagct tatcgggatg gtgttcactc atcagcttac
    29581 ggtaggcacg tttgatggtg gtcgcatcat ccgtcggctt cacgcccagc acattacagg
    29641 catcttccag cgtcgggcca cgctgcgctt gctgccagtt accaccgcca gattgctgct
    29701 gataaccgcc gccaaactgt gcaccgccct gcatcatgcg caaaaactgg tcaaactggg
    29761 cgcgggagat ccctaattct tcagcaatga catacagcac cgcccgttca ttcgggtgca
    29821 gtgaaccatc agcaaacgcc gcctgaatct ggatctccag aaacatacga attaagtcaa
    29881 aacgaccaaa gcagacactg cgaaactggc gcatcttttc gcgcagcggg taattatctg
    29941 attttcccac ccggaacgcg ttttgcgccg cagtacggga agcgccatga aggttcattc
    30001 ggtccatcaa ctggctggcg atatgaatat cagcctccgt gacgcgacct ttggatttgg
    30061 ttaaatgccc catcacttca aaagtggtgg caaaaaacag cgcctgacgc tcacgctggt
    30121 tggcgaacca cgccatttta cggctacggg ctttatcaaa catatggcca attaacaggc
    30181 ctaacactac gccccaaaag ccgccgccca tcagtaaggc cacggccacg ccaatgattt
    30241 ttccccaata ctgcatatat tccccaaatc gacacacgga tatcagggct atctcccaca
    30301 atataaaggt gcttttaccg ttttccggct gcggtcaatt gtgggacatc gcctataatt
    30361 tgcattatca tacctgtcat tcaatgccgt gcctaacacc actgacgcta ttcggacagg
    30421 attaacacta gcgtagagat gacgagtacg ttagtctctg accgtttgtc acgcgcaacg
    30481 ttaccgatga tggaacaata aaatcaacgt atgaaaaaac gtatccccac tctcctggcc
    30541 accatgattg ccaccgccct ttatagtcaa cagggactgg cagccgacct cgcctcacag
    30601 tgcatgttgg gcgtgccaag ctatgaccgt cctctggtac agggcgatac caatgactta
    30661 cccgtgacta tcaatgctga ccacgcgaaa ggggactacc cggatgacgc cgtgtttact
    30721 ggcagcgtgg atatcatgca gggtaacagc cgtctgcagg ccgacgaagt gcagctccat
    30781 caaaaagagg caccaggaca accggagccg gtacgtaccg ttgatgcgct cggtaatgtc
    30841 cattacgacg ataaccaggt gatcctcaaa gggccgaaag gctgggcgaa tctgaacacc
    30901 aaagatacca acgtctggga aggtgattac cagatggtgg gtcgccaggg tcgcggtaaa
    30961 gcggacctga tgaaacaacg tggcgaaaac cgctatacca ttctggataa cggtagcttt
    31021 acctcctgtc tgccgggttc tgacacctgg agcgtggtag gtagcgaaat tattcatgac
    31081 cgcgaagaac aagttgctga gatctggaac gcccgcttta aggtgggtcc ggtaccgatc
    31141 ttttatagcc cctatttgca gttgccggtg ggtgacaaac gtcgctctgg tttcttgatc
    31201 ccgaacgcca agtacaccac caccaactac tttgagttct acctgccata ttactggaac
    31261 atcgcgccaa atatggatgc caccatcacg ccgcattata tgcatcgtcg tggcaacatc
    31321 atgtgggaga acgaattccg ctacctctcc caggcgggcg ctggcttgat ggaactggac
    31381 tatctgcctt cagataaagt ctatgaagat gaacacccga acgatgacag ttcacgtcgt
    31441 tggttattct actggaacca ctccggggtc atggatcagg tgtggcgttt caacgtcgac
    31501 tacaccaagg tcagcgatcc tagctacttc aatgatttcg ataacaagta cggttccagt
    31561 actgacggct acgcaacgca aaaattcagc gttggctatg cggtgcaaaa cttcaatgcc
    31621 accgtttcaa ccaagcagtt ccaggttttc agcgaacaga acaccagtag ctactcggca
    31681 gagccgcagt tagacgttaa ttactaccag aatgatgttg gtccgtttga tacgcgtatt
    31741 tacggccagg cagtgcactt tgttaacacc agagacgaca tgcctgaagc aacccgtgtt
    31801 cacctggaac cgaccatcaa tttgccgctc tctaataact ggggcagcat caataccgaa
    31861 gcgaagtttc tggcaaccca ttatcagcaa accaatcttg actggtataa ctccagaaac
    31921 acgaccaagc tggacgaatc cgttaaccgc gtaatgccgc aattcaaagt tgacggcaaa
    31981 atggtctttg aacgcgatat ggaaatgctg gctccgggtt atacccaaac gctggaaccg
    32041 cgcgcgcagt atttgtacgt gccgtatcgc gatcagagcg acatctataa ctacgactcg
    32101 tctctgctgc aatctgacta ctctggcctg ttccgggacc ggacttacgg cggtcttgac
    32161 cgtattgcct ccgctaacca agtgacgacc ggtgtcacat ctcgcatata tgatgatgct
    32221 gccgttgaac gttttaatat ttccgttggt caaatctact atttcacgga gtctcgcact
    32281 ggcgatgaca acataacatg ggagaatgac gacaaaacgg gctcactggt gtgggcaggc
    32341 gacacctact ggcgtatctc cgagcgttgg ggattgcgtg gcgggattca gtacgataca
    32401 cgtctggata acgtagcgac cagtaactcc agcattgaat accgtcggga tgaagaccgt
    32461 ctggtacagc tgaattacca ttacgccagc ccggaatata ttcaggctac gctgcctaag
    32521 tactattcca ctgctgagca atataagaat ggtatttcgc aggtaggtgc tgtcgccagc
    32581 aggccaattg ccgatcgttg gtccattgtt ggggcctact actacgacac caatgcgaac
    32641 aagcaagccg actctatgtt aggtgtgcaa tacagctcct gctgctatgc aattcgcgtc
    32701 ggttacgagc ggaagctgaa cggttgggat aacgataaac aacatgcggt atatgacaac
    32761 gcaatcggct ttaacatcga acttcgcggc ctgagctcca actacggtct gggtacgcaa
    32821 gagatgctgc gttcgaacat tctgccgtat caaaacactt tgtgatttgt tgatttacca
    32881 cgtaatccgc agtgcggtta attgaaatgg aaaaagtatg aagaactgga aaacgctgct
    32941 tctcggtatc gccatgatcg cgaataccag tttcgctgcc ccccaggtag tcgataaagt
    33001 cgcagccgtc gtcaataacg gcgtcgtgct ggaaagcgac gttgatggat taatgcagtc
    33061 ggtaaaactg aacgctgcgc aggcaaggca gcagcttcct gatgacgcga cgctgcgcca
    33121 ccaaatcatg gaacgtttga tcatggatca aatcatcctg cagatggggc agaaaatggg
    33181 agtgaaaatc tccgatgagc agctggatca ggcgattgct aacatcgcga aacagaacaa
    33241 catgacgctg gatcagatgc gcagccgtct ggcttacgat ggtctgaact acaacactta
    33301 tcgtaaccag atccgcaaag agatgattat ctctgaagtg cgtaacaacg aggtgcgtcg
    33361 tcgcatcacc atcctgccac aggaagtcga atccctggcg cagcaggtgg gtaaccaaaa
    33421 cgacgccagc actgagctga acctgagcca catcctgatc ccgctgccgg aaaacccgac
    33481 ctctgatcag gtgaacgaag cggaaagcca ggcgcgcgcc attgtcgatc aggcgcgtaa
    33541 cggcgctgat ttcggtaagc tggcgattgc tcattctgcc gaccagcagg cgctgaacgg
    33601 cggccagatg gggtggggcc gtattcagga gttgccgggg atcttcgccc aggcattaag
    33661 caccgcgaag aaaggcgaca ttgttggccc gattcgttcc ggcgttggct tccatattct
    33721 gaaagttaac gacctgcgcg gcgaaagcaa aaatatctcg gtgaccgaag ttcatgctcg
    33781 ccatattctg ctgaaaccgt cgccgatcat gactgacgaa caggcccgcg tgaaactgga
    33841 gcaaattgct gctgatatca agagtggtaa aacgactttt gctgccgcag cgaaagagtt
    33901 ctcgcaggat ccaggttctg ctaaccaggg cggtgatctc ggctgggcta caccagatat
    33961 tttcgatcca gccttccgtg acgccctgac ccgcctgaac aaaggtcaaa tgagtgcacc
    34021 ggttcactct tcattcggct ggcatttaat cgaactgctg gatacccgta atgtcgataa
    34081 aaccgacgct gcgcagaaag atcgtgcata ccgcatgctg atgaaccgta agttctcgga
    34141 agaagcagca agctggatgc aggaacaacg tgccagcgcc tacgttaaaa tcctgagcaa
    34201 ctaatggtta aaacccaacg tgttgtgatc actcccggcg agcccgccgg gattggcccg
    34261 gacttagttg tccagcttgc acagcgtgag tggccggtcg aactggttgt ttgtgccgat
    34321 gccactctcc ttaccgaccg ggcagcgatg ctcggtttgc cgctcaccct ccgcccttat
    34381 tcccccaact cccctgcaca accgcaaact acgggcacat taacgctact tcctgtcgcg
    34441 ctacgtgaat ctgtcactgc ggggcagtta gcgattgaaa atgggcatta cgtggtggag
    34501 acgctggcgc gagcgtgtga tggctgtctg aacggtgaat ttgctgcgct gatcacaggc
    34561 cccgtgcata aaggcgtcat taacgacgca agcattccgt ttaccggtca taccgagttt
    34621 ttcgaagagc gttcgcaggc gaaaaaagtg gtgatgatgc tggcgaccga agaacttcgc
    34681 gtggcgctgg caacgacgca tttaccgctg cgcgatatcg cagatgctat cacccctgca
    34741 cttttgcacg aagtgattgc tattttgcat cacgatttgc gtacaaaatt tggtattgcc
    34801 gaaccgcgca ttctggtctg cgggctgaat ccgcacgcgg gcgaaggcgg tcatatgggt
    34861 acggaagaga tagacaccat tattccggtg ctcgacgagc tgcgggcgca ggggatgaaa
    34921 ctcaacgggc cgctgcctgc cgataccctg tttcagccga aatatctcga taacgccgac
    34981 gccgtgctgg cgatgtacca cgatcagggt cttcccgtgc taaaatacca gggcttcggg
    35041 cgcggtgtga acattacgct gggcctgccc tttattcgca catcagtgga ccacggcacc
    35101 gcgcttgaac tggcgggacg tggcgaagcc gatgtcggca gttttattac ggcgcttaat
    35161 ctcgccatca aaatgattgt taacacccaa tgaataatcg agtccaccag ggccacttag
    35221 cccgtaaacg cttcgggcaa aactttctca acgatcagtt cgtgatcgac agcattgttt
    35281 ctgccattaa cccgcaaaag ggccaggcaa tggtcgaaat cggccccggt ctggcggcat
    35341 tgaccgagcc ggtcggcgaa cgtctggacc agctgacggt catcgaactt gaccgcgatc
    35401 tggcggcacg tctgcaaacg catccattct taggcccgaa actgacgatt tatcagcagg
    35461 atgcgatgac ctttaacttt ggcgaactgg ccgagaaaat gggtcagccg ctgcgtgttt
    35521 tcggcaacct gccttataac atctccacgc cgttgatgtt ccatctgttt agctatactg
    35581 atgccattgc cgacatgcac tttatgttgc aaaaagaggt ggtgaatcgt ctggttgcag
    35641 gaccgaacag caaagcgtat ggtcgattaa gcgtcatggc gcaatactat tgcaacgtga
    35701 tcccggtact ggaagttccg ccgtcagcct ttacaccacc acccaaagtg gattccgccg
    35761 tcgtgcgcct ggtccctcat gcaacgatgc ctcacccggt taaagatatt cgcgtgttga
    35821 gccgcatcac caccgaagcc tttaaccagc gtcgtaaaac cattcgtaac agcctcggca
    35881 acctgtttag cgtcgaggtg ttaaccggaa tggggatcga cccggcgatg cgagcggaaa
    35941 atatctctgt cgcgcaatat tgccagatgg cgaactatct ggcggagaac gcgcctttgc
    36001 aggagagtta acgatgatca attcgccccg agtgtgtatt caggttcaaa gcgtctacat
    36061 tgaggcacaa tcttcacctg ataatgaacg ttacgttttt gcttataccg taaccatacg
    36121 caatctgggg cgagcgccag tgcagttgtt ggggcgttac tggctgatca ccaatggcaa
    36181 tggccgtgaa accgaagtcc agggcgaagg agtggttggc gtccagccac ttatcgcgcc
    36241 tggcgaagag taccagtaca ccagcggtgc aatcattgaa accccgctgg gcaccatgca
    36301 gggtcactac gaaatgatcg atgaaaatgg cgtccctttc agcatcgaca ttcccgtatt
    36361 ccgactcgcc gttcccacac tcattcatta aaagaatatg gcgacatacc ttattggcga
    36421 cgttcatggt tgttacgatg aactgatcgc attgctgcat aaagtagaat ttacccccgg
    36481 gaaagatacc ctctggctga cgggcgatct ggtcgcgcgc ggcccgggtt cgctggatgt
    36541 tctgcgctat gtgaaatcct taggcgacag cgtacgtctg gtgctgggca atcacgatct
    36601 gcatctgctg gcggtatttg ccgggatcag ccgcaataaa ccgaaagatc gcctgacacc
    36661 gctgctggaa gcgccggatg ccgacgagct gcttaactgg ctgcgtcgcc agcctctgct
    36721 gcaaatcgac gaagagaaaa agctggtgat ggcccacgca gggatcacgc cgcagtggga
    36781 tctgcagacc gccaaagagt gcgcgcgcga tgtagaagcg gtgctgtcga gtgactccta
    36841 tcccttcttt cttgacgcta tgtacggcga tatgccaaat aactggtcac cggaattgcg
    36901 ggggctggga agactgcgtt ttatcaccaa cgcctttacc cgtatgcgtt tttgcttccc
    36961 gaacggtcaa ctggatatgt acagcaaaga atcgccggaa gaagcccctg ccccactgaa
    37021 accgtggttt gcgattcctg gccctgtcgc tgaagaatac agcatcgcct ttggtcactg
    37081 ggcatcgctg gaaggcaaag gtacgccgga aggtatttac gcgctggata ccggctgctg
    37141 ctggggtggg tcattaacct gcctgcgctg ggaagataaa cagtattttg tccagccgtc
    37201 gaaccggcat aaggatttgg gcgaagcggc ggcgtcttaa acacagcctg atacaggaag
    37261 gccggataag acgcgaccgg cgtcgcatcc ggcactagcc gtaaattcta tacaaactta
    37321 ccgccgctcc agaatctcaa agcaatagct gtgagagttc tgcgcatcgg cgtcgtggaa
    37381 ttcgctgaat accgattccc agtcatccgg ctcgtaatcc gggaaatggg tgtcgccttc
    37441 cacttctgcg tcgatatgcg tcagatacag tttttgcgct tttggcagga actgctcata
    37501 aacgcgaccg ccgccaatca ccatgatttc tggtacgtca ccacacgccg caatggcttc
    37561 atccaccgat ttcacccacg ttacgcgatc gtccgtaccc ggttgactgc tgaggataat
    37621 atttttgcgt cctggcaacg gacgaccgat tgattcccaa gtatggcggc ccataatcac
    37681 gggtttattt aaggtgttgc gtttaaacca ggcgagatcg gcaggcaggt tccacggcat
    37741 ggcgttttcc atgccgataa cgcgatctac cgctaacgcc gcaatcagac tgatcattga
    37801 gatttcccga taaaaaaatt gtcgccacta tacgtaaagc gtaaaccgtc gtcgactggt
    37861 gcgaggatga tgttgaggaa aattttatat tctgctggcg agtccacgct ctccccctgg
    37921 actcgccgca ttacaatgaa acaaaaacaa acagttagct gtaaagtgtg atttacgtca
    37981 ctctttatta agatgagggt ttcgtttccg gttcatccgc catgttgccg gtatgtttac
    38041 cttcttctgt tccctgccag ccatgtcgtt gaattaatga cagatgttcg cggtcctcgg
    38101 tgatgatctc acttaacatc gcgctggtgc gtttatagac cgccgcgcgg gctttggtgt
    38161 cattctcaac cattgccatc tcttccacca tctgaatatt aaagcggcgg aacacatcgg
    38221 cacgttctcg cgcttcatac ggccccagac ctaaactttc cagcgccaga cgcccggttt
    38281 tcagcgcacc ttcaaaggtt tcacgctccg gcttttcaac gcctgcctga cgcaaacgaa
    38341 tgtagtgatc gacatcgcgg gcgcgggcaa taatctgcaa atgcgggaaa tgttctttca
    38401 ccatctccgt cagttgcagg ttggtttgcg gatcgtcaat agcgttaatc agcacttccg
    38461 ctttcgccgc tccggcagat tccagtaaat ccatccgcgt ggcatcgcca taaaacactt
    38521 tcataccaaa tttacgcagc gtttcgatat ggtccggatc gtgatcgagc accaccattt
    38581 tcaccccgct ggagagcagt aaacgtccgg taatctgtcc aaaacgaccg aatccggcga
    38641 taatcacgcg cggctgttct tcatcgatct catcggcttc acgcgcctcc tccgtagaag
    38701 attgctcaag gcgattgagg atcaccagca gaatcggcgt tgctgccatc gacagcgcca
    38761 ccgccagggt cagcgatttc gcccactccg gctccagcac attcgccatc tgcgccgcac
    38821 caaataccac aaaggcaaac tcactgccct gccctaacaa caccgcaaac cagcgacgct
    38881 gtttatttgg cacctgcaac ggtcgggcaa tcagccacag catggcgatt ttgatgatga
    38941 ggaaaccgag cagcaaaatg acaatgcgca atgggttttc aatcagcgtg ccaaagtcta
    39001 tcgacatgcc aacaccgatg aaaaacagcc ccaacagcaa acctttaaac ggttcgatat
    39061 cgctttccaa cgcatgacgg tattccgagc ttgccagcag tacgcccgcc agaaacgcgc
    39121 ccatcgccat cgacaagccg acctcttcca gcagcaaacc aaagccaaac acaaggaata
    39181 acgccacggc actaaacact tcccgcaagc cagagcgggc aacaaaacgc agcgccggac
    39241 gcgtaacata gcgccccagc aataccacca gtaccagcgc acccgccact tttaacgccg
    39301 agagagcaaa tgcgcccatc gtcgtcgagg cactgctcgc cgccagtagc gggatcatcg
    39361 ccaccagcgg gattgccgcg atatcctgga acagcagcac tgcaaaggca ctgcggccca
    39421 tttgcgtcac catcagattg cgttcattca tcgcctgcat ggcaatcgcc gttgatgaga
    39481 gcgccagcgt cataccgatc aattccgcga cctgccagcg cagcccaagt aacatacaga
    39541 acagccccag caggccaccg caaatcacca tctgcaatgc gccaccgccg aacactgccg
    39601 cacgcagctt ccacagcctt tgtggatcga gttcgaggcc gataataaac agcatcagca
    39661 ccaccccaat ctcggcaaag tgcagaatag attcggcatc ggtcaccagt cgcagccccc
    39721 acgggccaat aatgcagccg gcgatcaggt agccaagtac cgatcccaga ccaagacgta
    39781 ccgcaatggg tacaatcagc gccgccgatc cgaggtagat cagcgcctga accagcgtat
    39841 gactatccat gatgggcctc ctgccattcc agcagacgtt gcttatagtg gcgcgcctgc
    39901 ccttcgaggg tttcgtcgtc acaaataaag gtgcagtgca tggcaaacgg tggcagccag
    39961 ttcagcccgc agtagattgc cgtcgcctgt agcggctgcg acagcacatc aaagcccgga
    40021 tgcgcaccaa tttcaaaatg gctttccccg ccgccggtcg tcaccgccca cagcaaatgt
    40081 ttgccatgca gcgccgtgcc gccgtgaccg taagcccagc cgtgcgacaa aactttatcg
    40141 atccaaagtt taagaagcgg aggaatgctg taccactgca tcgggtgctg ccagacgatc
    40201 agatcggcgc gagagagcgc ctcctgctcg gcggcaatat cgatattgaa gtcaggatag
    40261 agttgataaa gagagcgaat ttcgacgcct tccagcgtcc ttgcctgttc aagcatccgt
    40321 ttattcgcat gggaatgatg cggatacgga tgcgcataaa tgatgaggat catagttatt
    40381 agcccgcgtg tttacgcctt tgttttccca caaagtgtag tcatatattc acgcggctta
    40441 tagtcaataa tattgattca gaaaagagag aaatctgaac aaataatccc attctctgcc
    40501 cgcggatcgt catattgctg gtctgcgcca gtgacattcc tcgagtctcc ggggcaaacg
    40561 ctacggaaat caacaagcca aacagcgaga tacccgcccc cattagcatc gtgttactga
    40621 taccgtaatt attgataaat atcggcagtg cccaggtcga aacaatggtg ccaatacgac
    40681 ttaaggacat aatcacgccc acggcagagg cgcggatatc cgtcgggaag agttcattag
    40741 gatagagcca ctgcaaatta cccggcccgc cagagaaaaa ggcatacacc gcaaaggcca
    40801 tcactaccag ccagatcccc atatccggga ttagccccaa aaccgccaac gccagcgtca
    40861 tcatggcaaa gctgccaatc aacaatggac gccgtccggc agtgtttagc cacagcatcg
    40921 gcggaataca gccgagcata aagaacaggc taatcaccac attccctagt gccgcgtttt
    40981 tgccaacccc caatcccaac gaaccaacga tttgcgggcc aaaggtgtaa atggcgaaca
    41041 ttgggatcac ctggcaggtc cagatggcgg caacaaacag aacaaaagga aaatggcggc
    41101 gattaaacag atcgcgaaaa cgggtttgct gcggctgctc ttcatcgaaa gccaccggtt
    41161 cgccaaacag ttttatcatc atctcttcgc actcttttac tcgccctttg cgtaataacc
    41221 agcggggaga ttctggcagt tcgaatcgac caatcaaaat caacaaacaa gggatcgccg
    41281 cgctacccag catccagcgc caaccacctt ccacatcata aagccagtag ccgaccagat
    41341 cggcgcaggt cgcgccgaca taccacatcg cggcaataaa gctgatggaa aacgcccgct
    41401 gacgggtact ggagaactcg gtgatcatcg aagtggcgat gggataatct gcaccgatga
    41461 caatgccgat gagtacccgc atcaccaaca gttcgacggg ggatgaaaca cacatcgtcg
    41521 ccaccgatat cacgccgatg gcgatgatat caatgaggaa cattttgcgc cgtccgactt
    41581 tatcggagat atagccaaac agcgatgtgc caacgaacag cccggcgagc gttcccgcgc
    41641 ccagcaagcc aatccagtca gcgtccagtt tcagcgccgg agtcagttgc tccagcgcca
    41701 cgccaatcat taccagtaca taaccatcca gaaacggacc accgcttccc cacagcaaaa
    41761 tgcggcggtg aatagaggag aatttgagat cgtcaaagtt tctggacggt tgcatgggtt
    41821 attcctgttt ttttgcaatg agagtcagta cggcgttacg tatcaaacca acatcagccg
    41881 taacggaact ccacaccaaa ggtgccgcgc gggtattccc actgttccag cgccgacccc
    41941 agccccagaa tgcgacaggt gccgcactcc agacatccgg cgtaatcgaa gcgcacactg
    42001 ccgtcatcct gcttcttgta cagacctgcg gggcacgctt tcaccagcag ctccagcacc
    42061 tgtttatcag catcggcctt cacaacaatg tgcggatgct cttcatcgac attgaattta
    42121 ttgacgccca gtttgacgtc cacattgacg ggagaagtca taaaacggtc actcctttca
    42181 tgccatcctt gatcagattg atgaagccca ctttcttgcc gtggcggagg attttcttgc
    42241 gcatcagttc cggcgcactg ccgtcaatgg tgaacaggtc acgcgccacg cccaccgcca
    42301 gttccgggtt gccgctaaac atgcgtgggt tatcaaggaa cgccggtagt ttctggtaca
    42361 tgcgcatatc gcgcagcggg ccactctcca gatgctgacg atattccgcg agtttttgct
    42421 tactgaaatc gtcgcttttc atcgctgaaa gcacggtttt tgctgcagct tccccggcgg
    42481 caatcgccag atccataccg cgaatggtaa aaccgaggtt catacacatt ccggcggcat
    42541 caccggcaat caatacgccg tcaccgacca gctccggcag catgttgatg cctgcttccg
    42601 gcactacgtg agcggaatat tccaccagct tgccgcccgc gatcagcggt gcaacggccg
    42661 gatgctgttt gaaatcttcc agcatttgcg gcaccgattt tttcgcgtca tgcagatgat
    42721 gcagaccaca aaccagcccc agcgacaggg tattttcatt ggtataaagg aagccgccgc
    42781 ccatcaggcc atcggtgggt gatcccgcaa acagacaagc cgcgccctga ttaccctgca
    42841 actgaaaacg gtcttcgatt accgacttcg gtaactcgat cagttccttc acgccaaccg
    42901 ccacatccgt cggtttaacg cgttttgcca tccccaactt ttcggcaagg atggagttca
    42961 ccccatcagc aaggagcacc gttttcgctt caatcacatc accatcggct tctacaccga
    43021 cgactttgcc atcgcgctgt acgaggttat cgacgcggat cccggtaatt aactgcgcgc
    43081 ccgcttcttc ggcctgctcc atcaaccagg catcaaattt actgcgcaaa acggagtaag
    43141 aacgctggga tggcgaggtt tcgtcaccgt tgcagtagtc catagtcatc gctgactttt
    43201 ccgtcataaa cgcgagtttt tcatgggtga tcaggcgttc tacgggggcg gaatcggcga
    43261 aacctggaat aatgtgttcc agactgtggg catagagacg cccgccagta acgttcttgg
    43321 cacctgcgga attgccacgc tcgataacta acacttgcgc cccttcgcgg gcgagcacca
    43381 gtgcggcaac cgaaccggca agccctgcac cgacgatgat ggcgtcaaag atatcttcgg
    43441 acataagagc tccctgtcag cggcagagcc gtaatgcagc cctgccagag tggatcaacg
    43501 cgctaaagct gcggtcagtg ccggaaggat cttcacggca tcgccaacaa tgccgtaatc
    43561 cgcgtactgg aagatcggcg catttttatc tttattgatg gcgaaaatgg tttgcgacgc
    43621 gttagcgcca accatgtgct ggatctaccc ggagatcccc accgccaggt acagttcagg
    43681 tttcagcatc aggttggaga taccgacata gcgttcgtgc tccatccatt tttcgttttc
    43741 tgccaccgga cgagaacagg ccaactccgc acctatcgcc ttgcaaagct gttctgccag
    43801 cgcaatgttc tctttgctgc caatgccgcg accgacgctg accaccagac gggctttgtc
    43861 gagatcgacg ctgttgctct ggcgcgcctg ggttgccgtg cgggtaatcg ccacagccgg
    43921 agcctgccac tccacggtgt gcgtttcgcc agtgcgtgac gcgtctggct gagccgcatc
    43981 gaacgtgccg ctgctgatgg tcagtaccgc atacggcgtg gcaatgcgtt cttcgccaat
    44041 cgccagaccg ccgtaaacca tgtgtttcac tgttgcttta ccgtcctgta cgctgacggt
    44101 gctggcatcg ttagacaccg ccgctttaag gcgataaccc agttttgccg ccagtaattt
    44161 gccgcgacgg gtgtttggca acagcaccag accgtcagcg ccgtgctggc gaatagtgtc
    44221 agccatgaca ccggcgtaat cttcgatcat ccgatcgtcc ggtttgccgt ttaatttcca
    44281 gacatgatta gcgccgagct ggattgcctg tgcgccgtcg gcatcattga ggacaaaggc
    44341 attgatttga ttagctaaag cctgcgcacc gttcatcagt tccggcagac gagagggggt
    44401 atcgctgaat acccagactt gagaaaacgt gttcatagca tcccctgtaa ttaaatgact
    44461 ttgcgaagat tttcagcaaa tgcggcgatc tgttcttcgc cgtcgccttc aatcacgatg
    44521 cgctgacgct cgcgctgttt cggcgcggca acctgttgtt cagaccaggc gtccacggcg
    44581 ttaaaaccaa tatccgccgc cgaccatacc tggactggct ttttcgccgc gccgagaatg
    44641 gctttcatcg aaggaatttg tggggagttg atatcagtgg aaacagcaac aaccgcaggc
    44701 agcggaatgc ttaaggtttc ggtttcatct tccagttcgc gctcaacggt gagggtatct
    44761 gccgtcaggg agataatttt gctgacgccg ttaactgccg gaatattgag gatttcgccc
    44821 accagcagac caacctgctg ggcataaagg tcggaagaac catcgccaca gaggatcaga
    44881 tcaaagcctg ctttctgggc ggctgcagcc agtgcgctcg ccgtttgttg cggcagtgcc
    44941 tgctcgaact ggtcatcaat caccacaatc agttcatccg ggccgcgcga tagcacatct
    45001 ttacgccctt tggcgttggt cagggcttta ccgcccacac ttaaggctgt cacctgcgcc
    45061 tctgctgcct gttgctttag ctggcaagcc gcttcaatag cgttgagatc gtattggctt
    45121 attttggcat cggctttgct gaagtctaat gaaccatcag cattattgac cgcaatatcc
    45181 tgttcatcag gcacgcactt atagcaagta ataatcttca ttgcatctcc agaaatcatg
    45241 aaggaaacgt cgttcatatc ggtggtgtta attacagata tcacgccctt taatttggaa
    45301 ataaaacttt aatcaccaat attgaaagtc agatgctatt tttttaccca caaaagaata
    45361 taacaaactg attaataacc aaaattaacg aaaaacgccc tctgttcact cgaaacaaaa
    45421 atgtgatacc aatcacagaa tacagcttat tgaataccca ttatgagtta gccattaacg
    45481 cgtccacgat gttaataata attatattaa atgttaacaa aaataaaaca aacgggaacg
    45541 caaaataaat attcgttttc acagtggaat taactcatga agaatgaaaa gagaaaaacg
    45601 ggaatagaac cgaaggtttt ctttccgcca ttaataatcg tcggcatact ttgttggctt
    45661 acagtcagag atctggatgc ggcgaatgtc gttattaatg ctgtattcag ttacgtcacc
    45721 aatgtatggg gatgggcatt tgaatggtat atggtggtga tgcttttcgg ttggttctgg
    45781 ctggtgtttg gcccgtatgc caaaaagcgt ttaggtaacg aaccgccaga atttagcacc
    45841 gccagttgga tctttatgat gttcgcctcc tgtacgtctg ctgccgtact gttctgggga
    45901 tcgattgaga tctactacta catctccacc ccgccgtttg gcttagaacc gaactcgaca
    45961 ggagcgaaag agttggggct ggcttacagc ttgttccact ggggacctct gccgtgggcc
    46021 acttacagct tcctttcagt cgccttcgct tacttcttct ttgtccgcaa aatggaagtg
    46081 attcgcccca gctcgacact ggtgccgctg gcaggtgaaa aacacgccaa agggttgttc
    46141 ggtaccatcg tcgacaactt ctatctcgtc gccttgatct tcgcgatggg taccagtctg
    46201 ggccttgcca cgccgctggt gaccgagtgt atgcaatggt tgtttggcat tccgcatacc
    46261 ctgcaactgg acgctatcat cattacctgc tggattatcc tcaacgccat ttgcgtcgct
    46321 tgcggtctgc aaaaaggggt acgtatcgcc agtgacgtgc gtagttacct gagcttcctg
    46381 atgctgggtt gggtgttcat cgtcagcggt gccagcttca tcatgaacta cttcaccgat
    46441 tcggtgggga tgttgctgat gtatctgccg cgcatgttgt tctataccga tcccatcgct
    46501 aaaggcggct tcccgcaggg ctggaccgtg ttctactggg catggtgggt gatttacgcc
    46561 atccagatga gtatcttcct cgcccgcatc tcccgtggtc gtactgtgcg tgaactgtgc
    46621 ttcggcatgg tgatggggct gacagcatcc acctggatcc tgtggaccgt gctcggtagt
    46681 aacactctgc tgttgatgga taaaaacatc atcaacattc caaatctgat cgaacagtac
    46741 ggtgtggcgc gcgccatcat cgaaacctgg gccgctctgc cactcagcac cgccaccatg
    46801 tggggcttct tcatcctctg ctttattgcc accgttacgc tggttaacgc ctgctcttat
    46861 accctggcga tgtctacttg ccgcgaagta cgcgatggtg aagaaccacc gctgctggtg
    46921 cgtatcggtt ggtcaattct ggttggcatt atcggtattg ttctgctggc gctcggcggc
    46981 ctgaaaccga ttcaaaccgc cattatcgcc ggaggatgcc cgctgttctt cgtcaacatt
    47041 atggtgacgc tctcctttat taaagacgcg aaacagaact ggaaagatta attaaccccc
    47101 aaaatatcaa gaggttgaaa gatggatttt aatttaaatg atgagcagga actgtttgtc
    47161 gccggtatcc gcgaactgat ggccagcgaa aactgggagg cctattttgc cgagtgcgac
    47221 cgtgacagcg tctacccgga acgttttgtc aaagcactgg cggatatggg tatcgacagt
    47281 ctgctgatcc ctgaagagca cggtggtctg gacgcggggt ttgttactct cgccgccgtg
    47341 tggatggagc tgggacgtct gggggcacca acctatgtac tgtaccagct gccgggcggg
    47401 ttcaatacct tcctgcgcga aggcacacaa gagcagatcg acaagattat ggctttccgc
    47461 ggcaccggta agcagatgtg gaactcagcg attaccgaac cgggcgcggg ctccgacgtg
    47521 ggtagcctga aaacgactta tacccgtaga aatggtaaga tttatcttaa tggtagtaag
    47581 tgttttatta ccagtagcgc ctacaccccg tacatcgtgg tgatggcgcg cgacggggct
    47641 tctccggaca aacctgtcta caccgaatgg tttgttgata tgagcaaacc gggcatcaaa
    47701 gtgaccaaac ttgagaagct cggtctgcgt atggatagct gctgtgaaat cacctttgac
    47761 gatgtggaac tggacgagaa agacatgttc ggtcgggaag gtaacggctt taaccgcgtc
    47821 aaagaagagt tcgaccatga acgtttcctg gtagccctga ccaactacgg tacggcgatg
    47881 tgcgcctttg aagatgctgc gcgctacgcc aaccagcgcg tgcagtttgg cgaggctatt
    47941 ggtcgtttcc agttgattca ggaaaaattc gcccacatgg cgatcaaatt aaactccatg
    48001 aaaaacatgc tgtatgaagc agcgtggaaa gcagacaacg gcaccatcac ctctggcgat
    48061 gcagcgatgt gcaaatactt ctgcgccaat gcggcatttg aagtggtgga tagcgcaatg
    48121 caggtgctgg gcggtgtcgg gattgcgggc aaccaccgca tcagccgctt ctggcgtgac
    48181 ctgcgtgtag accgcgtttc cggaggatct gacgaaatgc agatcctgac gctgggtcgt
    48241 gcggtgctga agcaataccg ctaagtccgt gcgctgcctg atgcgacgct gacgcgtctt
    48301 atcaggccta cgggagcacg aatgtaagcc ggataaggcg tttacgccgc atccggcagt
    48361 catgcgctac ataaattttt caggagctaa taatggatca tctacccatg ccgaaattcg
    48421 gaccgctggc cggattgcgc gttgtcttct ccggtatcga aatcgccgga ccgtttgccg
    48481 gacaaatgtt cgcagaatgg ggcgcggaag ttatttggat cgagaacgtc gcctgggccg
    48541 acaccattcg cgttcaaccg aactacccgc agctctcccg ccgcaatttg cacgcgctgt
    48601 cgttaaatat tttcaaagat gaaggccgcg aagcgtttct gaaattaatg gaaaccaccg
    48661 atatcttcat cgaagccagt aaaggtccgg cctttgcccg tcgtggcatt accgatgaag
    48721 tactgtggca gcacaacccg aaactggtta tcgctcacct gtccggtttt ggtcagtacg
    48781 gcaccgagga gtacaccaat cttccggcct ataacaccat cgcccaggcc ttcagtggtt
    48841 acctgattca gaacggtgat gttgaccagc caatgcctgc cttcccgtat accgccgatt
    48901 acttttctgg cctgaccgcc accacggcgg cgctggcagc actgcataaa gtgcgtgaaa
    48961 ccggtaaagg cgaaagtatc gacatcgcca tgtatgaagt gatgctgcgt atgggccagt
    49021 acttcatgat ggattacttc aacggcggcg aaatgtgccc gcgcatgagc aaaggtaaag
    49081 atccctacta cgccggttgc ggtctgtata aatgtgccga cggctacatc gtgatggaac
    49141 tggtgggcat tacccaaatt gaagagtgct ttaaagatat tggcctcgca catctactcg
    49201 gtacgccgga aatcccggaa ggcactcagc ttatccaccg tatcgaatgc ccttacggcc
    49261 cactggtaga agaaaaactc gatgcctggc tggcggcaca taccatcgca gaagttaaag
    49321 aacgctttgc cgaactgaat atcgcctgtg ccaaagtact gaccgtaccg gaactggaaa
    49381 gcaatccaca gtatgttgcc cgtgaatcaa tcactcagtg gcaaacgatg gatggtcgca
    49441 cctgcaaagg gccgaacatc atgccgaaat tcaaaaataa ccccggacaa atctggcgcg
    49501 gaatgccctc acatggcatg gacacggctg ccattttgaa aaatatcggc tacagcgaaa
    49561 acgacattca ggagttggtc agcaaaggtc tggccaaagt tgaggactaa acgcgtggcg
    49621 tcagtcggct tgcctgacta gcgcccgccc aataacaaac gaacaaaaat ggatagaggt
    49681 gcaatggata tcattggcgg acaacatcta cgtcaaatgt gggacgatct ggcggacgtt
    49741 tacggtcata aaacggcgct gatttgtgaa tccagcggcg gagtcgttaa ccggtatagt
    49801 tatcttgagt taaatcagga gattaaccgc acggcaaacc tgttttatac gctggggatt
    49861 cgcaaaggca acaaggttgc actacatctc gacaactgcc cggaatttat cttttgctgg
    49921 ttcgggctgg caaaaattgg cgcgattatg gtgccgatta acgcccgcct gttacgcgag
    49981 gaaagcgagt ggatcttgca aaatagccag gcgtgcctgc tggtgaccag tgcgcaattt
    50041 tatcctatgt atcaacagat tcagcaggaa gatgccactc aattgcggca catttgcctg
    50101 acagatgtgg cacttcccgc tgatgatgga gtgagttcat ttactcaact gaaaaatcaa
    50161 caacctgcca ccttgtgcta tgcaccgccg ctatcgactg acgatacggc ggaaattctc
    50221 ttcacctccg gcaccacctc ccgaccgaaa ggtgtggtga ttacccatta caacctgcgc
    50281 ttcgctggat attactccgc ctggcagtgt gcactgcgtg acgatgacgt ctacatgacg
    50341 gtaatgcctg cgtttcatat cgattgccag tgtactgcgg cgatggcggc gttttctgcc
    50401 ggggccacct ttgtgctggt cgagaaatac agcgcccgcg ccttctgggg acaggcgcag
    50461 aagtaccgcg ccaccattac cgaatgtatt ccgatgatga tccgtacgtt gatggtgcag
    50521 ccgccttcag cgaacgatcg gcaacaccgc ctgcgggaag tgatgtttta tctcaacttg
    50581 tcggagcagg aaaaagacgc attttgtgaa cgcttcggcg ttcgcttgct gacgtcttat
    50641 gggatgacgg aaaccattgt gggcattatc ggcgatcgcc ctagcgataa acgacgctgg
    50701 ccgtcgattg gtcgggcggg gttttgctac gaagcggaga tccgcgacga tcacaatcgc
    50761 ccgctcccgg caggtgagat cggtgaaatc tgtattaaag gcgtaccagg gaaaaccatc
    50821 ttcaaagagt attttctcaa cccgaaagcc actgcgaaag tgctggaagc cgacggctgg
    50881 ctacataccg gcgataccgg ataccgcgac gaagaaggct ttttttattt cgtcgatcgc
    50941 cgctgcaata tgatcaaacg cggtggcgag aatgtctcct gcgtggagct ggaaaatatc
    51001 atcgccaccc atccgaaaat tcaggacatc gtggttgtgg gtattaaaga ttcgattcgc
    51061 gatgaagcca tcaaagcatt tgtggtgctg aatgaaggtg aaacattgag cgaagaggaa
    51121 ttttttcgct tctgcgaaca aaatatggcg aaatttaaag tgccctctta tctggagatc
    51181 agaaaagatc tgccacgtaa ttgctcgggg aaaataatta gaaagaatct gaaataaatg
    51241 aaacgtcagg gaactaccct gccagctaat aatcacgccc taaaacaata tgcatttttt
    51301 gcagggatgt tgtcgtccct aaaaaagcaa aaatggagaa aaggaatgag tgaatcatta
    51361 catctgaccc gcaatggatc aattctggaa attacccttg atcgcccaaa agcgaatgct
    51421 attgatgcaa aaaccagctt tgaaatgggc gaagtatttc taaatttccg tgacgatccg
    51481 caattacgtg tcgccattat taccggtgcc ggagagaaat tcttttccgc gggctgggat
    51541 ttaaaagcgg cagcagaagg cgaagcaccg gatgctgact ttggcccggg tggttttgcg
    51601 ggattaaccg aaattttcaa tctcgacaaa ccagttatcg cagctgtgaa cggctacgcc
    51661 tttggcggcg gtttcgaact ggcgctggcg gcagatttta ttgtttgtgc cgataacgcc
    51721 agcttcgccc tgccggaagc caaactgggc atcgttcctg acagcggcgg tgtgctgcgt
    51781 ctgccgaaga tcctgccgcc taccatcgtc aatgaaatgg tgatgaccgg cagacgaatg
    51841 ggcgcagaag aggcgctgcg ttgggggata gtcaaccgcg tggttagcca ggcggaactg
    51901 atggataacg cccgcgaact ggctcagcag ctggttaaca gcgccccgct ggcgattgcg
    51961 gcgctgaaag agatctaccg caccaccagc gaaatgccgg tagaagaagc gtatcgctat
    52021 attcgcagcg gcgtgttgaa acactatcca tcggttctgc attcggaaga tgccattgaa
    52081 gggccgctgg cgtttgccga gaagcgcgat ccggtgtgga aaggacgtta acaaccgtga
    52141 gctattacgc ctttgagggg ttaattccgg tggttcaccc gacggcgttt gtccatccca
    52201 gtgccgtctt gattggcgat gtgattgtgg gagccggtgt ctacatcggc ccactcgcct
    52261 cactgcgtgg tgactacggg cggttgatcg tgcaagcggg agccaatatt caggatggct
    52321 gcattatgca tggctactgc gacactgaca ccatcgttgg ggaaaacggc catatcgggc
    52381 atggagcgat cctgcatggt tgtgtgattg gtcgcgatgc attggtcggg atgaacagcg
    52441 taattatgga tggcgcggtc attggcgaag agagcattgt tgccgcaatg agctttgtca
    52501 aagcaggctt tagtggcgag aaacgccagt tgttgatggg tacgccagcc cgcgctgtac
    52561 gcagtgttag tgacgacgag ttacactgga aacggttgaa taccaaagag tatcaggatc
    52621 ttgtcgggcg ctgtcatgca tcgttacatg aaacgcagcc actgaggcaa atggaggaaa
    52681 atcgcccccg tttgcagggg acgacggatg tgacgcctaa acggtgatct tgccggatgc
    52741 gacctggttg ctggataaga tgctacaaca tcgtatctgg caagcaggac gcccggtatc
    52801 aacgagataa aattaacgac gcatactctt tgacaacatc atctgccact tctgctcgcg
    52861 gttaagttcc gtgagcggaa cggcaggaat acgcggtgtc tttcttacgc cgactaattt
    52921 ttcccgactg ttattgcgca ggcgcgcgta aatttgctca tcgatatcga ccactttaac
    52981 cagtcgctga cactggcatc cccgcccttc cagcttatta gggatcatct taacttcgca
    53041 gcttatttct gtgacttccg acagaatata agtcagggta ttaaccgcct tgctgtgttc
    53101 aatatcgaaa tgaatagaga tctctcttgc tatcacccac cgattttcag ccatcatcca
    53161 ttcggcgatt aacaagtaga gtggtttttc aacatatcct tcacacatac gaactccagt
    53221 ttcacaaatt aaaagtctag ctatgactcg ttttgaagca attaaacaag gccatattaa
    53281 aattgtggat atatcgatag tatgcaactt caccgtcgat aagtgtgagc taaatcctgc
    53341 ttatgtcatc aaaaatatcg attccccaaa agatttactt aatgggcaaa aaaaaacggt
    53401 cctcatcaga gaaccgtatt agttattcga tcaaattagc ggatgaaaaa tatccgccac
    53461 gacacgctat tatttgatct gtgcgtgcat ttcctgcacc gaaattactt tttcagtcgc
    53521 atcggcattc agcgccatcg cggtagcgaa accaccgttc agggtggtgt cataatgcac
    53581 tttatattgc agcgcactgc gacggatcac gcgggagtct tcaatcgcac gacggcctga
    53641 ggtggtgttg atgatgtagg tatattcgcc attcttgata cggtcctgaa tgtgcggacg
    53701 gccttcatgc accttgttta ccagacgcgg attgataccc gcttcgccca gcacaatcgc
    53761 cgtgccgtgg gtcgcatcca gctcgaagcc ctgtttcagc agttttgccg ccaggtccac
    53821 cacgcgttct ttatcgcctt cgcgcacgga aagcagcgca cgaccgtgtt tcttcatggt
    53881 ggagttgctg cccagctgcg ctttggcgaa cgcttcagcg aaggtgcggc ccacgcccat
    53941 gacttccccg gtagagcgca tttctggccc taacagcggg tcaacgcctg ggaatttatt
    54001 gaacggcagc accacttctt tcaccgagta gtacggcgga ataacttctt tggttacgcc
    54061 ctgctcagcc agcgatttgc cagccatcac acgcgccgcc acttttgcca gcggtacgcc
    54121 ggtggctttg gagacgaacg gaacagtacg cgccgcacgc gggtttactt caatcaggta
    54181 aacttcgttg tttttcaccg caaactgcac gttcatcaga ccgcgcacct gcaattcgaa
    54241 ggccagtttc tgcacctgct ggcgcatcac atcctgaatt tcctgactta aggtgtacgc
    54301 tggcagcgaa catgcggagt caccggagtg cacacccgcc tgctcgatgt gctccatgat
    54361 gccgccaatc agcaccattt cgccgtcgca gatggcatcc acgtcaactt ctactgcgtc
    54421 atcaaggaaa tgatccagca acactggcgc atcgttagac acgctaaccg ccgtctggaa
    54481 gtagcgacgc aggtcagctt cgtcatagac gatttccatc gcccgaccgc cgagaacgta
    54541 agacggacgc accaccagcg ggtagccaat ctcttttgcc ttctcaactg ccatttcaat
    54601 agcagtaacg gtggcgttcg ccggttgttt cagtttcaga cggtcaaccg catgctggaa
    54661 gcgttcacgg tcttccgcac ggtcgatagc atccgggctg gtgccgataa ccggtacgcc
    54721 agcggcttcc agtgcacgcg ccagtttcag cggggtctga ccgccgtact gaacgataac
    54781 gcctttcggc ttctcaatac gcacgatttc cagcacatct tccagagtta ccggctcgaa
    54841 gtagaggcga tcggaagtgt cgtagtcggt agagacggtt tccgggttac agttaaccat
    54901 aatggtttcg taaccgtctt cacgcagcgc cagcgaggcg tgtacgcagc agtagtcgaa
    54961 ttcgataccc tgaccgatac ggtttggacc gccgccaagc accatgattt tttcacggtc
    55021 ggtagacgga ttcgcttcgc actcttcttc ataagtggag tacatgtaag cggtgtcggt
    55081 ggcaaactct gccgcacagg tatccacgcg cttgtagacc gggtgcaggt catattggtc
    55141 acgcagctta cggatttccg cttcgcgtac gcccgccagt tttgccaagc gcgcatcggc
    55201 aaagcctttg cgtttcagct ggcgcaggaa ttcagcgttc aggccagtga tgcccacttc
    55261 cgccactttc tcttccagac gcaccagttc ttcaatctgt accaggaacc agcggtcaat
    55321 gttagtcagg ttgaagacgc cgtccacaga caggcccgcg cggaaagcat cggcgatgta
    55381 ccagatacgc tctgcgcctg cgtctttcag ttcgcgacgg attttggtta acgcttccgg
    55441 gtcatccagg ctcactttcg ggtcgaagcc agttgcgccc acttccaggc cgcgcagcgc
    55501 tttttgcagg gattcctgct gcgtgcgacc aatcgccatc acttcgccaa ccgatttcat
    55561 ctgagtggtc agacggtcgt tagcaccggc gaatttttcg aagttgaagc gaggaatttt
    55621 agtaaccaca tagtcgatgg acggctcgaa ggaggccgga gtacgtccgc cagtgatgtc
    55681 gttcatcagt tcgtctaggg tgtaacccac cgccagtttc gccgccactt tagcaatcgg
    55741 gaaaccggtc gctttcgacg ccagcgccga agaacgggac acgcgtgggt tcatttcgat
    55801 aacaatcagg cgaccgtttt tcgggttcac cgcaaactgg acattggaac caccggtttc
    55861 aacgccgatt tcacgcagca ccgccatcga ggcgttacgc atgatttgat attctttgtc
    55921 ggtcagcgtt tgggctggcg cgacagtgat ggagtcaccg gtgtggatgc ccatcgcatc
    55981 aaagttttcg atagagcaga cgatgatgca gttgtcgttt ttatcacgca ccacttccat
    56041 ctcgtactct ttccagccga tcagcgactc atcaatcagc aactctttgg tcggagagag
    56101 atccagaccg cgggcgcaaa tttcttcaaa ctcttcacgg ttataagcga taccgccgcc
    56161 gctaccgccc atggtaaagg atgggcgaat aatgcacggg aagcccacgt cagcggcaac
    56221 agccagcgct tcttccatcg tgtgtgcgat accggaacgc gcggtttcca gaccaatttt
    56281 cttcatcgct acgtcgaaac gacggcggtc ttctgcttta tcaatcgcat cggcagtggc
    56341 accaatcatg gtgacaccga actcttccaa cacgccctga cgttccagct ccagcgcgca
    56401 gttcagcgcc gtctgaccgc ccatggttgg cagcaccgcg tccgggcgct ctttttcaat
    56461 aatcttgcgt accacttccc agtgaatcgg ctcgatgtag gtcgcatcgg ccatttccgg
    56521 gtcggtcatg atggtcgccg ggttggagtt caccagaatg acgcggtaac cctcttcgcg
    56581 cagggcttta catgcttgcg cgccagagta gtcaaactca cacgcctgac cgataacaat
    56641 cgggcccgca cccagaatca ggatactttt tatatctgta cgttttggca tggctctttt
    56701 actcctgatt acttagcggt tttacggtac tgctcaatta acttgataaa gtggtcgaac
    56761 aacggcgcgg cgtcgtgtgg accagggctg gcttcagggt gaccctggaa gctgaacgcc
    56821 ggcttatcga tgcgatgaat gccctgtaac gtaccgtcga acagggattt atgcgtgaca
    56881 cgcaggtttg caggtaatgt tgcttcatcc accgcaaaac cgtggttctg ggcggtgatc
    56941 atcaccacgt ttttctccac atctttaacc ggatggttgc cgccgtggtg accaaatttc
    57001 attttgacag tcttcgcacc gctcgccagc gccagcagct gatgaccgag acagatgccg
    57061 aataccggaa tatcggtttc gaggaacttc tggatggcgg taatggcgta atcgcacggg
    57121 gccgggtcgc caggaccgtt ggagaggaag atgccgtctg gattcatttt cagcacatct
    57181 tccgcagaag tttgcgccgg aacgatggtc aggcgacagc ctctatccac cagcatccgc
    57241 aggatgttgc gcttggcacc aaaatcataa gccacgacgt ggaacggcag ctcgtcttct
    57301 tttttcgctt ctggcaggcc gccggtcaac gtccagctcc cttgtgtcca gctataggct
    57361 tctgcggtgg tcacttcttt tgccagatcc atgccgttca gacctgggaa cgcgcgggct
    57421 ttttctaacg ccagcgccgc atccgggtta tcgcctgcga taatgcagcc attctgtgcg
    57481 cctttctcgc gcagtaaacg cgtcagctta cgggtatcga tatcggcaat cgccacgatg
    57541 ttatggcgct tcaggtaaga agagaggtct tcggtattac ggaagttgct ggcaatcagc
    57601 ggcaggtcgc gaatcaccag accttgtgca tgtacctgag aagattcttc atcggcgtca
    57661 ttagtgccga cattgccaat atggggataa gtaagagtaa cgatttgacg agaataggaa
    57721 ggatcagtga ggatttcttg ataaccggtc attgaagtat tgaaaacgac ttccccaacc
    57781 gccgaacctg ttgcccctat ggcccgaccg tgaaactggg ttccgtcttc cagaaccaat
    57841 agcgctgact taatcaaaac accctccaga gaatactcac tcactttatt tgcatattaa
    57901 ttcacagtga tgacgtaaat caatgcaaat ctgcttaccg acgaatttct ggcaaacggc
    57961 ggcattctgg agatatgggc gctaaaagtc aacttaatgg caggattttt tatattattt
    58021 tgtggctcat gcatgtttta caggcattaa gcgacaaaaa catcatctaa cctgttctgg
    58081 aaaacgcttg cgcagcaaga agattgcatg aaataaaaac gagcaggaaa aagtggacca
    58141 aatggtcaaa atttacatta aaacaacaaa aatagaagac gaaacatgta atttttggca
    58201 gttatagaaa atttaaaagc ataacaccaa aaataaaagg gctaaaaatt aaagcccttt
    58261 ataattaata aattaaatgt gttacttttg catcataata aacattttgt ggttacaaat
    58321 tattgagatc aagcacatct cgcatatcaa aaagaccact ttccttacca ctcaaccaca
    58381 aagccgatct taccgcaccg ttagcaaatg tcatgcggct ggacgcctta tgggtgatct
    58441 ccagacgctc gccaatatcg gcaaacatcg cggtatgttc gccaacgatg tcacctgcac
    58501 gcacggtagc aaaaccaatg gtacctggca cacgttcacc ggtatggcct tcacgactgt
    58561 agaccgcgca atctttcaga tctttatcaa gggcgtgggc gatcgcctct cccattgcca
    58621 gtgcggtgcc tgacggcgca tcaactttat gtctatgatg tgcttcaata atttcgatat
    58681 cggtgtagtc acccatcact ttggctgctt tctccagcag cttaagcatg acgttaacgc
    58741 caacgctaaa attggcggca aagacaatcg caatatcggc ggcggcgtca cgaattgcct
    58801 gtttaccggc ttcgtcaaac cccgtagtgc cgatcaccat ccctttgcca tgctgccgac
    58861 aaaaagcgag atggttcagc gtaccttccg gacgggtaaa atcgataaac acatcaaaat
    58921 catcttttat cgcatcaaga ctgctttgca cggtaacgcc tgttttccct gcaccagcca
    58981 gctcaccggc gtcgctgccc agtaaagaag atccttcgcg ctccagcgcc gcgcccagct
    59041 gcacgccttc taatgccagt gccgcctgaa tcaactggcg gcccatacgc cccccggctc
    59101 ccgcgatggc aacgcggatg tttgcatcat gcatagctat tctcttttgt taatttgcat
    59161 agaccgtttt cagagtaacc agcgcatcgc tacgccgcca gccaaaaacg ctgataatta
    59221 gaaattaatg ataaacaggg agggatatca gtgaaaggca tgagtcgatg aatgactgca
    59281 tgccgataac atgtgacagg ttacggcacc agagccagca cctcagccac ccactgttgg
    59341 aagcctttta cattcagatc caatgccacc tgtacattgg ctggtttgcc caggcaaccg
    59401 tcgatatcaa ccaccgtcgt gcctgaggta aattcgcctt gagtttctac cgcgacaaaa
    59461 cacggcttga gggtgaataa gtccgggcgc accagccagg cgatggcgca gagatcgtgc
    59521 attcgcagac cgctttgcat actgccgcta cggtagtggc taaacagggc gtggagcatt
    59581 tttccggtgc gattcaactc cggtaaagta gcgagatagt caggggttaa tattgcctga
    59641 ttggtgacat ccagaccgca catgacaatt tcgatgccgc tacggaagac acaggcagcc
    59701 gcttctggat cggcagcgat attaaactcg gcatttggtg tacagttgcc gcgaccagca
    59761 gaaccgccca tgatcaccag acgacgaata tacggtttac attccgggca ttgtaaaagt
    59821 aacagcgcaa tattggttaa cgggccgatg gccaccaggg taacgggctc tggtgcacgc
    59881 atcagggcat cccgaatcgc cagaaacgcc ggtatcccga gtggctttcg gttgtgctca
    59941 acaaagtcgt agccagccat tcccgattcg ccgtgcacag atgctgcatc acgcggtgcg
    60001 cgtaccagcg gcacagcggc tccctgggcg agcggaatct ccgcattcca gaaatgcagc
    60061 agttgcaggg cattgcgggt agttttctca accgagacat tacccgcgac ggtggtcatc
    60121 agttgcaggt cgagttcggg ggcaaaaatc gcggcggcaa tggcgacggc atcgtcaatg
    60181 ccggggtcag tatcgaggaa gataggtaaa cgcatgtttt cttcataaaa aatgccggta
    60241 acaagaccgg cattttttat gaagaaaaca tgccggtaac aagaccggca ttttcgcata
    60301 acttaggctg ctaatgactt aatcgacttc acgaatatcg acacgcagct ctttcggcac
    60361 ttcgaaaaca atattttctt cacggccttc cagcggaatg gcttcaccac cacccaactg
    60421 ctgcaaacgt gccaccacat tctgcaccag aatatccgga gccgatgcgc ccgcagtcac
    60481 gccgacgcat ttaacctctt tcacccactc ttcctggata tctttcgcat cgtcaatcaa
    60541 aaacgcgtgt ttgcccatac gctgggccag ctccgccaga cggttggagt tggaggagtt
    60601 tttcgaaccg accaccaaca caacttccgc ctgttctgcc agggcgcgta ccgcttcctg
    60661 acggttagtc gtggcgtagc agatgtcatc tttgcgcgga ccgacaattt tcgggaagcg
    60721 tttacgcagc gcgtcgatca catcagacgt gtcatccacc gacagcgtgg tttgggtcat
    60781 aaaggagagc ttctcttcgt ttttgaccgt cagtttccac acatcgtctg gcgattcgac
    60841 tagatacatt cccccttcgg ggttgctgta ctgccccatc gtcccttcca cttccgggtg
    60901 cccggcgtgg ccgatgagaa tagattcttc gccacggcga ctggcgcggg caacttccat
    60961 atgcactttg gtcaccagcg gacaggtggc atcaaacacc gtcaaatcgc ggcttttcgc
    61021 ttcgttacga accgcctgag aaacaccgtg tgcggagaaa atcaggatcg cgccgtccgg
    61081 cacttcgcta atctgctcaa taaagatagc cccacgctcg cgcaagctat cgaccacgta
    61141 gcggttatgc accacttcgt gacggacata tatcggtgcg ccgtaaatgg ccagcgcgtt
    61201 ttcaacaatg ctgatagcgc ggtctacccc ggcacaaaaa ccacgcgggt tggccaacag
    61261 gatctgcatg ttacgcctcc agtgccggat cgatttccag cacttcaata tcaaaatgaa
    61321 cggtctgccc ggccagcgga tggttgaaat caacggtaat ggagtcgccg ttaatttcgc
    61381 ggatcacacc aggcatctca ctgccatcca ttgcggtaaa aagcatgatt gcgccaattt
    61441 ctggctcgcc tgcatccata aattcacggc gggagaagta ctgaatcagg tccggtgacg
    61501 gcacgccaaa cgccgcatct ggctccagcg agaaggtggt tttgtcgccc actttcagcc
    61561 ccagcaggtg ttgctccagc ccttcagaaa gagaagcatc gcccaggcgg aacagcgccg
    61621 gtttaccgtt gttgcgagta gactcggcgg tggtgccatc gtcgagtttt agcgtgaagt
    61681 gcaccaggac ggcgctattg ctctgtacag attcagacat gcaggtttgc tcttattttt
    61741 gaggattttt gccggatgcg gcctacgatt tcgtagcaat ctgtaggccg catccggcag
    61801 gatttattat tgttttttcg ctttggaagg taaaaaccct tccagcacga tcagtgccgc
    61861 accgacacaa atggcagtat cggcaaggtt gaaggtggcg aagtgccagt cgccgacgta
    61921 gaagtcgatc atatcgacaa cgaagccgtg ccacaggcgg tcgaacaggt tgcccagcgc
    61981 gccgccaata atcagcgcgt aagcgatatt gtttagcttc tgcgtggcct tcgagcgata
    62041 catcatcact gccaggagca cgctaatacc aatcgcaata ccggcaaaga accaacgctg
    62101 ccagccgccg ctatcggcaa ggaaactaaa cgccgcgcca tagttacgcg cataatgcag
    62161 attaagcgac gggaacagcg ggaccgtatc ccccagagca aagttctgga ggatcaggta
    62221 tttgctgccc agatcgataa tcagcacgac taccaccagc cacagccagc gtagccctgt
    62281 tgaacagatc gattgactca tcaggcaaac ttacgttttt caccgtcacc ggcgacgttg
    62341 ctgacacagc ggccgcagat ttctgcgtgt tccgccacct tgccgacatc ctgggtgtag
    62401 tgccagcagc gtgggcactt ctcaccttcg gctttactca acgcgacttt cagccctttg
    62461 agtacttcgc tctgctgagc atcagcaggt gcgtcgttat agtctgcaac ggtagcgccg
    62521 gaggtcaaca ggacaaatcg taattcatcg cccagcgcgg tcagtttcgc cgccagttcc
    62581 ggttctgcat acaaggttac tgccgcttcc agcgagccac ccactttctt gtcggcacgc
    62641 gcttgctcaa tgactttgtt cacttcgcca cgcactttca acagctcgtc ccagaacgca
    62701 tcgttcattg cttcactgtc tgccagacca aacaggcctt cgtaccactc accggtgaag
    62761 acgtattttt cacgttcgcc cggcagatag ccccacactt catcagcggt gaaggagagg
    62821 attggtgcca tccagcgcac cagcgcttca gcgatgtgat acagcgcagt ctggcagcta
    62881 cgacgcgcca cgctatccgc tttggcggtg tactgacggt ctttgatgat gtcgaggtag
    62941 aaggaaccca tctcaacgga gcagaagcgc atcagacgct gtaccacttc gtggaaatcg
    63001 tatgcttcgt acgccttgag gatgtcttcc tgtgccgctt tcgcacaacc tacggcccag
    63061 cgatccagta ccaccatctc ttccggtttc accatatctt ttgctggatc aaaaccattc
    63121 aggtttgcca gcaggaagcg cgcggtgtta cggatacgac gatagctatc ggcagcacgt
    63181 ttcaggatct cgtcagaaac ggccatttca ccggtgtagt cggttgatgc cacccacaga
    63241 cgcagaatat ccgcgcccag tttgttcatc acatcctgcg gcgaaacggt gttgccgatg
    63301 gatttagaca tcttgcgacc ctgaccatcc acggtaaagc cgtgggtcag tacctgacga
    63361 tacggcgctt tgcctttcat cgcggtggag atcattaggg aagacatgaa ccaaccacgg
    63421 tgctggtcag aaccttccag atacatgtcc gctgcgtgac cggcaaattc cggacgcacg
    63481 tcaacaacag aagagtgggt agatccggag tcaaaccata cgtcgagagt atccggcact
    63541 ttcacatact ggtcagcttc gtcgccgagg atctctttcg catcgagatc ccaccacgcc
    63601 tggatgccgt caacttcaac gcgtttagcc acttcttcca tcagttcgag ggtacgcgga
    63661 tgcagctctt ccgtgtcttt gtgcacgaac agtgacatcg gtacgcccca ggtgcgctga
    63721 cgggagatac accagtcagg acggttagca accatcgact cgatacgcgc ctggccccag
    63781 tccgggagcc actgcacgcc tttgatctct ttcagtgact gcgcacgcag acctttctga
    63841 tccatgctga cgaaccactg cggtgtcgcg cggaagatga tcggcgtttt gtgacgccag
    63901 cagcacggat agctgtgctg cattttctca acgtgcagca gcgcgccttt ttcctgcagc
    63961 agcgcaacga cgatgtcgtt cgctttgaag acgttcacgc catccagcgt cggataagtg
    64021 cccggcagat aagtgccgtc cgggccaacc gggttagcgg tttccaggcc gtatttctga
    64081 ccgatcacat agtcgtccgg gccgtggcca ggcgcggtgt gaacggcacc ggtaccggca
    64141 tccagggtaa cgtgatcgcc gaggattgcc ggaacgtcga agcccataaa cggatgggta
    64201 aagcgcagca gctcaagctc tgcacctttt accgtgccga gaatggtgta atcggtcacg
    64261 ccgatacgct gcattacgct ttcaaccaga tctttcgcca gaatcacggc ctgaccgtcg
    64321 atctgcacca gcgcatagtc gaaatcaggt gcaatagaga ttgcgcggtt cgcaggcaga
    64381 gtccacggcg tagtggtcca gatcaccagc gagattgggc cgttaacgtt gcttacgcca
    64441 aatttcgctt tcagcgcatc ctgatcgacc gcctggaaag cgacgtcaat ggacggagaa
    64501 gttttgtcgt aatactcaac ttccgcttct gccagtgcag aacggcagtc tacgcaccag
    64561 tgcaccggct tcgcgccttt gtgcaggtga ccattgccga tgattttgcc cagcgcgcgg
    64621 atgatgttgg cttcagtttt gaagtccata gtcagatacg ggtgcgacca gtcgcccagc
    64681 acgcccagac ggataaagtc tttgcgttga ccgtcaacct gggtcgccgc gtattcgcgg
    64741 cacttggcgc ggaactcggc ggcggtgaat ttctcacccg gcttaccgta ttcttgctct
    64801 actttcagct cgatcggcag accgtggcag tcccagccag gcacatacgg cgagtcataa
    64861 ccggaaagcc ctttggactt cacgataatg tctttcagaa tcttgttaac cgagtgacca
    64921 atatgaatgc tgccattcgc ataaggaggg ccatcatgca gaatgaaggt ttttttgcct
    64981 tttttagccg cacggatgat gccgtacaga tcatcatcag tccaacgcgc cagcattccc
    65041 ggttcgcgct tggcgagatc gccacgcatc gggaaccctg tttccggcaa attcagggtt
    65101 gatttatagt cactcatcag attctcggtt ccgtgtttcg gtttgattac ataacaggct
    65161 taagccggtt ttgttagccc aaaaaattcg cgggcggtta attcatcacg cgcaatctgc
    65221 gctttcagtt cgtccagcga cgcaaatcgc tgctcattgc gtattttttt acgcagcact
    65281 acttgtatat ggcgaccgta aaggtccatt gcaacatcta acaaatgcac ttccagctgc
    65341 tggcgaatac cggcaaccgt tgggcgtgtt ccgatgtttg ccacgccggg taacggcttt
    65401 tcaccgaggc ccagcacttc taccgcataa acccctttca ccggggaaac ctgacggcgt
    65461 agcggtacat tcgccgtcgg gaaacctata gtgcgcccta attcatcacc gtggactaca
    65521 cgcccggaga tggcaaacgg gtgccccagt aaactttctg ccagagccag attgtcatca
    65581 gccagcgcct gacgcacggc ggtgctgctg atacgcacac caccttcgca aaaagtttgc
    65641 gtactggtga tatcgaagcc gtattccatg ccagctttct gtaataacaa gaaatcgcct
    65701 tcacgaccag cgccaaagcg gaaatcatca cctacggcaa gaaattttac gcgcaaatgc
    65761 ttcaccaaaa gatcgctgat gaaattttgc gcggttaacg ccgcaaaacg cctgtcgaaa
    65821 cgcacgcaca gcacgtaatc aacgccacac tctgcaaggt aacgcagttt ttcccgcagc
    65881 cgggtcagtc ttgccggggc tttatcggta gcaaacagtt ccagtggttg aggttcaaaa
    65941 agcatcacca tcaccggtaa gttgcgcttg cgcccttctt cctgcaagcc ctgtaacagc
    66001 gcgcgatgac cgcgatgcac gccgtcgaaa ttaccaatag tcagcacaca cccttcttgc
    66061 ggggcctggc tgagattatg tatgccgcgt atcagcttca tgtctggctc aaaacagtga
    66121 aaatcgtccg agtatacctt gtacagcggt caaggttaac cggcgattga gtaccgaaat
    66181 cagaaagaag tggcttttca ttgccatgga gcaaatcacg ggaagaaact gaccgcctgc
    66241 tgcaattttt atcgcggaaa agctgtattc acaccccgca agctggtaga atcctgcgcc
    66301 atcactacgt aacgagtgcc ggcacattaa cggcgcttat ttgcacaaat ccattgacaa
    66361 aagaaggcta aaagggcata ttcctcggcc tttgaattgt ccatatagaa cacatttggg
    66421 agttggacct tggctaatat caaatcagct aagaagcgcg ccattcagtc tgaaaaggct
    66481 cgtaagcaca acgcaagccg tcgctctatg atgcgtactt tcatcaagaa agtatacgca
    66541 gctatcgaag ctggcgacaa agctgctgca cagaaagcat ttaacgaaat gcaaccgatc
    66601 gtggaccgtc aggctgctaa aggtctgatc cacaaaaaca aagctgcacg tcataaggct
    66661 aacctgactg cacagatcaa caaactggct taatcgccaa tttgctgaag ctttgtgaaa
    66721 aagcccgcgc aagcgggttt ttttatgcct gctgcttttg tgatgcgtga gagaacacac
    66781 tgccggttgc cagatagtca aaagagacat gccgttgtac tatctgaact cttcgtggct
    66841 taagaatcgt agaaccatcg gctgatacat tctgaacaag aatatctact caatctccag
    66901 actcccccac aaagaatatg gatattgtga tacacattga ggtattaaac ttaagaccac
    66961 tctatttttg aattaaattc ctatatactt taaccattca taaatatctc ctcattagac
    67021 ctt
//
LOCUS       DAJTRC010000009        66634 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.009, whole genome shotgun sequence.
ACCESSION   DAJTRC010000009 DAJTRC010000000
VERSION     DAJTRC010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66634
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.009"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(22..2469)
                     /gene="glgP"
                     /locus_tag="OO357_000640"
     CDS             complement(22..2469)
                     /gene="glgP"
                     /locus_tag="OO357_000640"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen phosphorylase"
                     /protein_id="HCR5885694.1"
                     /translation="MNAPFTYSSPTLSVEALKHSIAYKLMFTIGKDPVVANKHEWLNA
                     TLFAVRDRLVERWLRSNRAQLSQETRQVYYLSMEFLIGRTLSNAMLSLGIYEDVQGAL
                     EAMGLNLEELIDEENDPGLGNGGLGRLAACFLDSLATLGLPGRGYGIRYDYGMFKQNI
                     VNGSQKESPDYWLEYGNPWEFKRHNTRYKVRFGGRIQQEGKKTRWIETEEILGVAYDQ
                     IIPGYDTDATNTLRLWSAQASSEINLGKFNQGDYFAAVEDKNHSENVSRVLYPDDSTY
                     SGRELRLRQEYFLVSSTIQDILSRHYQLHKTYDNLADKIAIHLNDTHPVLSIPEMMRL
                     LIDEHQFSWDDAFEVCCQVFSYTNHTLMSEALETWPVDMLGKILPRHLQIIFEINDYF
                     LKTLQEQYPNDTDLLGRASIIDESNGRRVRMAWLAVVVSHKVNGVSELHSNLMVQSLF
                     ADFAKIFPGRFTNVTNGVTPRRWLAVANPSLSAVLDEHLGRNWRTDLSLLNELQQHCD
                     FPMVNHAVHQAKLENKKRLAEYIAQQLNVVVNPKALFDVQIKRIHEYKRQLMNVLHVI
                     TRYNRIKADPDAKWVPRVNIFGGKAASAYYMAKHIIHLINDVAKVINNDPQIGDKLKV
                     VFIPNYSVSLAQLIIPAADLSEQISLAGTEASGTSNMKFALNGALTIGTLDGANVEML
                     DHVGADNIFIFGNTAEEVEELRRQGYKPREYYEKDEELHQVLTQIGSGVFSPEDPGRY
                     RDLVDSLINFGDHYQVLADYRSYVDCQDKVDELYELQEEWTAKAMLNIANMGYFSSDR
                     TIKEYADHIWHIDPVRL"
     gene            complement(2488..3921)
                     /gene="glgA"
                     /locus_tag="OO357_000641"
     CDS             complement(2488..3921)
                     /gene="glgA"
                     /locus_tag="OO357_000641"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HCR5885695.1"
                     /translation="MQVLHVCSEMFPLLKTGGLADVIGALPAAQIADGVDARVLLPAF
                     PDIRRGVTDAQVVSRRDTFAGHITLLFGHYNGVGIYLIDAPHLYDRPGSPYHDTNLFA
                     YTDNVLRFALLGWVGAEMASGLDPFWRPDVVHVHDWHAGLAPAYLAARGRPAKSVFTV
                     HNLAYQGMFYAHHMNDIQLPWSFFNIHGLEFNGQISFLKAGLYYADHITAVSPTYARE
                     ITEPQFAYGMEGLLQQRHREGRLSGVLNGVDEKIWSPETDLLLASRYTRDTLEDKAEN
                     KSQLQIAMGLKVDDKVPLFAVVSRLTSQKGLDLVLEALPGLLEQGGQLALLGAGDPVL
                     QEGFLAAAAEYPGQVGVQIGYHEAFSHRIMGGADVILVPSRFEPCGLTQLYGLKYGTL
                     PLVRRTGGLADTVSDCSLENLADGVASGFVFEDSNAWSLLRAIRRAFVLWSRPSLWRF
                     VQRQAMAMDFSWQVAAKSYRELYYRLK"
     gene            complement(3921..5216)
                     /gene="glgC"
                     /locus_tag="OO357_000642"
     CDS             complement(3921..5216)
                     /gene="glgC"
                     /locus_tag="OO357_000642"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HCR5885696.1"
                     /translation="MVSLEKNDHLMLARQLPLKSVALILAGGRGTRLKDLTNKRAKPA
                     VHFGGKFRIIDFALSNCINSGIRRMGVITQYQSHTLVQHIQRGWSFFNEEMNEFVDLL
                     PAQQRMKGENWYRGTADAVTQNLDIIRRYKAEYVVILAGDHIYKQDYSRMLIDHVEKG
                     ARCTVACMPVPIEEASAFGVMAVDENDKIIEFVEKPANPPSMPNDPSKSLASMGIYVF
                     DADYLYELLEEDDRDENSSHDFGKDLIPKITEAGLAYAHPFPLSCVQSDPDAEPYWRD
                     VGTLEAYWKANLDLASVVPELDMYDRNWPIRTYNESLPPAKFVQDRSGSHGMTLNSLV
                     SGGCVISGSVVVQSVLFSRVRVNSFCNIDSAVLLPEVWVGRSCRLRRCVIDRACVIPE
                     GMVIGENAEEDARRFYRSEEGIVLVTREMLRKLGHKQER"
     gene            complement(5234..7207)
                     /gene="glgX"
                     /locus_tag="OO357_000643"
     CDS             complement(5234..7207)
                     /gene="glgX"
                     /locus_tag="OO357_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HCR5885697.1"
                     /translation="MTQLAIGKPAPLGAHYDGQGVNFTLFSAHAERVELCVFDANGQE
                     HRYDLPGNSGDIWHGYLPDARPGLRYGYRVHGPWQPAEGHRFNPAKLLIDPCARQIDG
                     EFKDNPLLHAGHNEPDYRDNAAIAPKCVVVVDHYDWEDDAPPRTPWGSTIIYEAHVKG
                     LTYLHPEIPVEIRGTYKALGHPVMINYLKQLGITALELLPVAQFASEPRLQRMGLSNY
                     WGYNPVAMFALHPAYACSPETALDEFRDAIKALHKAGIEVILDIVLNHSAELDLDGPL
                     FSLRGIDNRSYYWIREDGDYHNWTGCGNTLNLSHPAVVDYASACLRYWVETCHVDGFR
                     FDLAAVMGRTPEFRQDAPLFTAIQNCPVLSQVKLIAEPWDIAPSGYQVGNFPPLFAEW
                     NDHFRDAARRFWLHYDLPLGAFAGRFAASSDVFKRNGRLPSAAINLVTAHDGFTLRDC
                     VCFNHKHNEANGEENRDGTNNNYSNNHGKEGLGGTLDLVERRRDSIHALLTTLLLSQG
                     TPMLLAGDEHGHSQHGNNNAYCQDNQLNWLDWSQASSGLTAFTAALIHLRKRIPALVE
                     NRWWEEGDGNVRWLNRYAQPLSTDEWQNGPKQLQILLSDRFLIAINATLEVTEIVLPA
                     GEWHAIPPFAGEDNPVITVVWQGPAHGLCVFQR"
     gene            complement(7204..9390)
                     /gene="glgB"
                     /locus_tag="OO357_000644"
     CDS             complement(7204..9390)
                     /gene="glgB"
                     /locus_tag="OO357_000644"
                     /EC_number="2.4.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-alpha-glucan branching enzyme"
                     /protein_id="HCR5885698.1"
                     /translation="MSDRIDRDVINALIAGHFADPFSVLGMHKTTAGLEVRALLPDAT
                     DVWVIEPKTGRKLAKLECLDSRGFFSGVIPRRKNFFRYQLAVVWHGQQNLIDDPYRFG
                     PLIQEMDAWLLSEGTHLRPYETLGAHADTMDGVTGTRFSVWAPNARRVSVVGQFNYWD
                     GRRHPMRLRKESGIWELFIPGAHNGQLYKYEMIDANGNLRLKSDPYAFEAQMRPETAS
                     LICGLPEKVVQTEERKKANQFDAPISIYEVHLGSWRRHTDNNFWLSYRELADQLVPYA
                     KWMGFTHLELLPINEHPFDGSWGYQPTGLYAPTRRFGTRDDFRYFIDAAHAAGLNVIL
                     DWVPGHFPTDDFALAEFDGTNLYEHSDPREGYHQDWNTLIYNYGRREVSNFLVGNALY
                     WIERFGIDALRVDAVASMIYRDYSRKEGEWIPNEFGGRENLEAIEFLRNTNRILGEQV
                     SGAVTMAEESTDFPGVSRPQDMGGLGFWYKWNLGWMHDTLDYMKLDPIYRQYHHDKLT
                     FGMLYNYTENFVLPLSHDEVVHGKKSILDRMPGDAWQKFANLRAYYGWMWAFPGKKLL
                     FMGNEFAQGREWNHDASLDWHLLEGGDNWHHGVQRLVRDLNLTYRHHKAMHELDFDPY
                     GFEWLVVDDKERSVLIFVRRDKEGNEIIVASNFTPVPRHDYRFGINQPGKWREILNTD
                     SIHYHGSNAGNGGTVHSDEIASHGRQHSLSLTLPPLATIWLVREAE"
     gene            complement(9663..10766)
                     /gene="asd"
                     /locus_tag="OO357_000645"
     CDS             complement(9663..10766)
                     /gene="asd"
                     /locus_tag="OO357_000645"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HCR5885699.1"
                     /translation="MKNVGFIGWRGMVGSVLMQRMVEERDFDAIRPVFFSTSQLGQAA
                     PSFGGTTGTLQDAFDLEALKALDIIVTCQGGDYTNEIYPKLRESGWQGYWIDAASSLR
                     MKDDAIIILDPVNQDVITDGLNNGIRTFVGGNCTVSLMLMSLGGLFANDLVDWVSVAT
                     YQAASGGGARHMRELLTQMGHLYGHVADELATPSSAILDIERKVTTLTRSGELPVDNF
                     GVPLAGSLIPWIDKQLDNGQSREEWKGQAETNKILNTSSVIPVDGLCVRVGALRCHSQ
                     AFTIKLKKDVSIPTVEELLAAHNPWAKVVPNDREITMRELTPAAVTGTLTTPVGRLRK
                     LNMGPEFLSAFTVGDQLLWGAAEPLRRMLRQLA"
     gene            10958..11551
                     /gene="yhgN"
                     /locus_tag="OO357_000646"
     CDS             10958..11551
                     /gene="yhgN"
                     /locus_tag="OO357_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YhgN"
                     /protein_id="HCR5885700.1"
                     /translation="MNEIISAAVLLILIMDPLGNLPIFMSVLKHTEPKRRRAIMVREL
                     LIALLVMLVFLFAGEKILAFLSLRAETVSISGGIILFLIAIKMIFPSASGNSSGLPAG
                     EEPFIVPLAIPLVAGPTILATLMLLSHQYPNQMGHLVIALLLAWGGTFVILLQSSLFL
                     RLLGEKGVNALERLMGLILVMMATQMFLDGIRMWMKG"
     gene            complement(11608..12948)
                     /gene="gntU"
                     /locus_tag="OO357_000647"
     CDS             complement(11608..12948)
                     /gene="gntU"
                     /locus_tag="OO357_000647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5885701.1"
                     /translation="MTTLTLVLTAVGSVLLLLFLVMKARMHAFLALMVVSMGAGLFSG
                     MPLDKIAATMEKGMGGTLGFLAVVVALGAMFGKILYETGAVDQIAVKMLKSFGHSRAH
                     YAIGLAGLVCALPLFFEVAIVLLISVAFSMARHTGTNLVKLVIPLFAGVAAAAAFLVP
                     GPAPMLLASQMNADFGWMILIGLCAAIPGMIIAGPLWGNFISRYVELHIPDDISEPHL
                     GEGKMPSFGFSLSLILLPLVLVGLKTIAARFVPEGSTAYEWFEFIGHPFTAILVACLV
                     AIYGLAMRQGMPKDKVMEICGHALQPAGIILLVIGAGGVFKQVLVDSGVGPALGEALT
                     GMGLPIAITCFVLAAAVRIIQGAATVACLTAVGLVMPVIEQLNYSGAQMAALSICIAG
                     GSIVVSHVNDAGFWLFGKFTGATEAETLKTWTMMETILGTVGAIVGMIAFQLLS"
     gene            complement(12952..13479)
                     /gene="gntK"
                     /locus_tag="OO357_000648"
     CDS             complement(12952..13479)
                     /gene="gntK"
                     /locus_tag="OO357_000648"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HCR5885702.1"
                     /translation="MSTTNHDHHIYVLMGVSGSGKSAVASEVAHQLHAAFLDGDFLHP
                     RRNIEKMASGEPLNDDDRKPWLQALNDSAFAMQRTNKVSLIACSALKKHYRDLLREGN
                     PNLSFIYLKGDFDVIESRLKARKGHFFKTQMLVTQFETLQEPGADETDVLVVDIDQPL
                     EGVVASTIEVIKKGK"
     gene            complement(13618..14613)
                     /gene="gntR"
                     /locus_tag="OO357_000649"
     CDS             complement(13618..14613)
                     /gene="gntR"
                     /locus_tag="OO357_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate operon transcriptional repressor GntR"
                     /protein_id="HCR5885703.1"
                     /translation="MKKKRPVLQDVADRVGVTKMTVSRFLRNPEQVSVALRGKIAAAL
                     DELGYIPNRAPDILSNATSRAIGVLLPSLTNQVFAEVLRGIESVTDAHGYQTMLAHYG
                     YKPEMEQERLESMLSWNIDGLILTERTHTPRTLKMIEVAGIPVVELMDSQSPCLDIAV
                     GFDNFEAARQMTTAIIARGHRHIAYLGARLDERTIIKQKGYEQAMLDAGLVPYSVMVE
                     QSSSYSSGIELIRQARREYPQLDGVFCTNDDLAVGAAFECQRLGLKVPDDMAIAGFHG
                     HDIGQVMEPRLASVLTPRERMGSIGAERLLARIRGESVTPKMLDLGFTLSPGGSI"
     gene            complement(14837..15532)
                     /gene="yhhW"
                     /locus_tag="OO357_000650"
     CDS             complement(14837..15532)
                     /gene="yhhW"
                     /locus_tag="OO357_000650"
                     /EC_number="1.13.11.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004213399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quercetin 2,3-dioxygenase"
                     /protein_id="HCR5885704.1"
                     /translation="MIYLRKANERGHANHGWLDSWHTFSCANYYDPNFMGFSALRVIN
                     DDVIEVGQGFGTHPHKDMEILTYVLEGTVEHQDSMGNKEQVPAGEFQIMSAGTGIRHS
                     EYNPSSTERLHLYQIWIMPEENGITPRYEQRRFDAVQGKQLVLSPDARDGSLKVHQDM
                     ELYRWALLKDEQSVHQIAAERRVWIQVVKGNVTINGVKASTSDGLAIWDEQAISIHAD
                     SDSEVLLFDLPPV"
     gene            complement(15655..16692)
                     /locus_tag="OO357_000651"
     CDS             complement(15655..16692)
                     /locus_tag="OO357_000651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5885705.1"
                     /translation="MVINCAFIGFGKSTTRYHLPYVLNRKDSWHVAHIFRRHAKPEEQ
                     APIYSHIHFTSDLDEVLNDPDVKLVVVCTHADSHFEYAKRALEAGKNVLVEKTFTPTL
                     AQAKELFALAKSKGLTVTPYQNRRFDSCFLTAKKAIESGKLGEIVEVESHFDYYRPVA
                     ETKPGLPQDGAFYGLGVHTMDQIISLFGRPDHVAYDIRSLRNKANPDDTFEAQLFYGD
                     LKAIVKTSHLVKIDYPKFIVHGKKGSFIKYGIDQQETSLKANIMPGEPGFAADDSVGV
                     LEYVNDEGVTVREEMKPEMGDYGRVYDALYQTITHGAPNYVKESEVLTNLEILERGFE
                     QASPSTVTLAN"
     gene            17025..17513
                     /gene="yhhY"
                     /locus_tag="OO357_000652"
     CDS             17025..17513
                     /gene="yhhY"
                     /locus_tag="OO357_000652"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5885706.1"
                     /translation="MSEIVIRHAETRDYEAIRQIHAQPEVYCNTLQVPHPSDHMWQER
                     LADRPGIKQLVACIDGDVVGHLTIDVQQRPRRSHVADFGICVDSRWKNRGVASALMRE
                     MIEMCDNWLRVDRIELTVFVDNAPAIKVYKKFGFEIEGTGKKYALRNGEYVDAYYMAR
                     VK"
     gene            complement(17572..19314)
                     /gene="ggt"
                     /locus_tag="OO357_000653"
     CDS             complement(17572..19314)
                     /gene="ggt"
                     /locus_tag="OO357_000653"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HCR5885707.1"
                     /translation="MIKPTFLRRVAIAALLSGSCFSAAAAPPAPPVSYGVEEDVFHPV
                     RAKQGMVASVDATATQVGVDILKEGGNAVDAAVAVGYALAVTHPQAGNLGGGGFMLIR
                     SKNGNTTAIDFREMAPAKATRDMFLDDQGNPDSKKSLTSHLASGTPGTVAGFSLALDK
                     YGTMPLNKVVQPAFKLARDGFIVNDALADDLKTYGSEVLPNHENSKAIFWKEGEPLKK
                     GDTLVQANLAKSLEMIAENGPDEFYKGTIAEQIAQEMQKNGGLITKEDLAAYKAVERT
                     PISGDYRGYQVYSMPPPSSGGIHIVQILNILENFDMKKYGFGSADAMQIMAEAEKYAY
                     ADRSEYLGDPDFVKVPWQALTNKAYAKSIADQIDIKKAKPSSEIRPGKLAPYESNQTT
                     HYSVVDKDGNAVAVTYTLNTTFGTGIVAGESGILLNNQMDDFSAKPGVPNVYGLVGGD
                     ANAVGPNKRPLSSMSPTIVVKDGKTWLVTGSPGGSRIITTVLQMVVNSIDYGMNVAEA
                     TNAPRFHHQWLPDELRVEKGFSPDTLKLLEAKGQKVALKEAMGSTQSIMVGPDGELYG
                     ASDPRSVDDLTAGY"
     gene            19434..19874
                     /locus_tag="OO357_000654"
     CDS             19434..19874
                     /locus_tag="OO357_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2756 family protein"
                     /protein_id="HCR5885708.1"
                     /translation="MKRLLILTALLPFVGFAQPINTLNNPNQPGYQIPSQQRMQTQMQ
                     TQQIQQKGMLNQQLKTQTQLQQQHLENQINNNSQRVLQSQPGERNPARQQMLPNTNGG
                     MLNSNRNPDSSLNQQHMLPERRNSDMLNQPSTPQPDIPLKTIGP"
     gene            complement(19861..20604)
                     /gene="ugpQ"
                     /locus_tag="OO357_000655"
     CDS             complement(19861..20604)
                     /gene="ugpQ"
                     /locus_tag="OO357_000655"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5885709.1"
                     /translation="MSNWPYPRIVAHRGGGKLAPENTLAAIDVGAKYGHKMIEFDAKL
                     SKDGEIFLLHDDNLERTSNGWGVAGELNWQDLLRVDAGSWYSKAFKGEPLPLLSQVAE
                     RCREHGMMANIEIKPTTGTGPLTGKMVALAARQLWAGMTPPLLSSFEIDALEAAQQAA
                     PELPRGLLLDEWRDDWRELTARLGCVSIHLNHKLLDKARVMQLKDAGLRILVYTVNKP
                     QHAAELLRWGVDCICTDAIDVIGPNFTAQ"
     gene            complement(20601..21591)
                     /locus_tag="OO357_000656"
                     /pseudo
     CDS             complement(20601..21591)
                     /locus_tag="OO357_000656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417907.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate import ATP-binding
                     protein UgpC"
     gene            complement(21593..22438)
                     /gene="ugpE"
                     /locus_tag="OO357_000657"
     CDS             complement(21593..22438)
                     /gene="ugpE"
                     /locus_tag="OO357_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000572177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpE"
                     /protein_id="HCR5885710.1"
                     /translation="MIENRPWLTIFSHTMLILGIAVILFPLYVAFVAATLDKQAVYAA
                     PMTLIPGTHLLENIHNIWVNGVGTNSAPFWRMLLNSFVMAFSITLGKITVSMLSAFAI
                     VWFRFPLRNLFFWMIFITLMLPVEVRIFPTVEVIANLKMLDSYAGLTLPLMASATATF
                     LFRQFFMTLPDELVEAARIDGASPMRFFCDIVFPLSKTNLAALFVITFIYGWNQYLWP
                     LLIITDVDLGTTVAGIKGMIATGEGTTEWNSVMAAMLLTLIPPVVIVLVMQRAFVRGL
                     VDSEK"
     gene            complement(22435..23322)
                     /gene="ugpA"
                     /locus_tag="OO357_000658"
     CDS             complement(22435..23322)
                     /gene="ugpA"
                     /locus_tag="OO357_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000099274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter permease
                     UgpA"
                     /protein_id="HCR5885711.1"
                     /translation="MSSSRPVFRSRWLPYLLVAPQLIITVIFFIWPAGEALWYSLQSV
                     DPFGFSSQFVGLDNFVTLFHDSYYLDSFWTTIKFSTFVTVSGLLVSLFFAALVEYIVR
                     GSRFYQTLMLLPYAVAPAVAAVLWIFLFNPGRGLITHFLAEFGYDWNHAQNSGQAMFL
                     VVFASVWKQISYNFLFFYAALQSIPRSLIEAAAIDGAGPIRRFFKIALPLIAPVSFFL
                     LVVNLVYAFFDTFPVIDAATSGGPVQATTTLIYKIYREGFTGLDLASSAAQSMVLMFL
                     VIVLTVVQFRYVESKVRYQ"
     gene            complement(23420..24736)
                     /gene="ugpB"
                     /locus_tag="OO357_000659"
     CDS             complement(23420..24736)
                     /gene="ugpB"
                     /locus_tag="OO357_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     substrate-binding protein UgpB"
                     /protein_id="HCR5885712.1"
                     /translation="MKPLHYTASALALGLALMGNAQAVTTIPFWHSMEGELGKEVDSL
                     AQRFNAENPDYKIVPTYKGNYEQNLSAGIAVFRTGNAPAILQVYEVGTATMMASKAIK
                     PVYDVFKEAGIQFDESQFVPTVSGYYSDSKTGHLLSQPFNSSTSVLYYNKDAFKKAGL
                     DPEQPPKTWQDLADYSAKLKASGIKCGYASGWQGWIQLENFSAWNGLPFASKNNGFDG
                     TDAVLEFNKPEQVKHIAMLEEMNKKGDFSYVGRKDESTEKFYNGDCAMTTASSGSLAN
                     IREYAKFNYGVGMMPYDADAKDAPQNAIIGGASLWVMQGKDKETYTGVAKFLDFLAKP
                     ENAAEWHQKTGYLPITKAAYDLTREQGFYEKNPGADTATRQMLNKPPLPFTKGLRLGN
                     MPQIRVIVDEELESVWTGKKTPQQALDTAVERGNQLLRRFEKSTKS"
     gene            complement(25133..25846)
                     /gene="livF"
                     /locus_tag="OO357_000660"
     CDS             complement(25133..25846)
                     /gene="livF"
                     /locus_tag="OO357_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivF"
                     /protein_id="HCR5885713.1"
                     /translation="MEKVMLSFDKVSAHYGKIQALHEVSLHINQGEIVTLIGANGAGK
                     TTLLGTLCGDPRATSGRIVFDDKDITDWQTAKIMREAVAIVPEGRRVFSRMTVEENLA
                     MGGFFAERDQFQERIKWVYELFPRLHERRVQRAGTMSGGEQQMLAIGRALMSNPRLLL
                     LDEPSLGLAPIIIQQIFDTIEQLREQGMTIFLVEQNANQALKLADRGYVLENGHVVLS
                     DTGDALLANEAVRSAYLGG"
     gene            complement(25848..26615)
                     /gene="livG"
                     /locus_tag="OO357_000661"
     CDS             complement(25848..26615)
                     /gene="livG"
                     /locus_tag="OO357_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000084170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter ATP-binding protein LivG"
                     /protein_id="HCR5885714.1"
                     /translation="MSQPLLSVNGLMMRFGGLLAVNNVNLELYPQEIVSLIGPNGAGK
                     TTVFNCLTGFYKPTGGTILLRDQHLEGLPGQQIARMGVVRTFQHVRLFREMTVIENLL
                     VAQHQQLKTGLFSGLLKTPSFRRAQSEALDRAATWLERIGLLEHANRQASNLAYGDQR
                     RLEIARCMVTQPEILMLDEPAAGLNPKETKELDELIAELRNHHNTTILLIEHDMKLVM
                     GISDRIYVVNQGTPLANGTPEQIRNNPDVIRAYLGEA"
     gene            complement(26612..27889)
                     /gene="livM"
                     /locus_tag="OO357_000662"
     CDS             complement(26612..27889)
                     /gene="livM"
                     /locus_tag="OO357_000662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     permease LivM"
                     /protein_id="HCR5885715.1"
                     /translation="MKPMHIAMALLSAAMFFVLAGVFMGVQLELDGTKLVVDTASDIR
                     WQWMFIGTAVVFFFQLLRPAFQKGLKSVSGPKFILPAIDGSTVKQKLFLVALLVLVVA
                     WPFMVSRGTVDIATLTMIYIILGLGLNVVVGLSGLLVLGYGGFYAIGAYTFALLNHYY
                     GLGFWTCLPIAGLMAAAAGFLLGFPVLRLRGDYLAIVTLGFGEIVRILLLNNTEITGG
                     PNGISQIPKPTLFGLEFSRTAREGGWDTFSNFFGLKYDPSDRVIFLYLVALLLVVLSL
                     FVINRLLRMPLGRAWEALREDEIACRSLGLSPRRIKLTAFTISAAFAGFAGTLFAARQ
                     GFVSPESFTFAESAFVLAIVVLGGMGSQFAVILAAVLLVVSRELMRDFNEYSMLMLGG
                     LMVLMMIWRPQGLLPMTRPQLKLKNGAAKGEQA"
     gene            complement(27886..28812)
                     /gene="livH"
                     /locus_tag="OO357_000663"
     CDS             complement(27886..28812)
                     /gene="livH"
                     /locus_tag="OO357_000663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010436488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter permease LivH"
                     /protein_id="HCR5885716.1"
                     /translation="MSEQFLYFLQQMFNGVTLGSTYALIAIGYTMVYGIIGMINFAHG
                     EVYMIGSYVSFMIIAALMMMGIDTGWLLVAAGFVGAIVIASAYGWSIERVAYRPVRNS
                     KRLIALISAIGMSIFLQNYVSLTEGSRDVALPSLFNGQWVVGHSENFSASITTMQAVI
                     WIVTFLAMLALTIFIRYSRMGRACRACAEDLKMASLLGINTDRVIALTFVIGAAMAAV
                     AGVLLGQFYCVINPYIGFMAGMKAFTAAVLGGIGSIPGAMIGGLILGIAEALSSAYLS
                     TEYKDVVSFALLILVLLVMPTGILGRPEVEKV"
     gene            complement(28860..29969)
                     /gene="livK"
                     /locus_tag="OO357_000664"
     CDS             complement(28860..29969)
                     /gene="livK"
                     /locus_tag="OO357_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="high-affinity branched-chain amino acid ABC
                     transporter substrate-binding protein LivK"
                     /protein_id="HCR5885717.1"
                     /translation="MKRNAKTIIAGMIALAISHTAMADDIKVAVVGAMSGPIAQWGDM
                     EFNGARQAIKDINVKGGIKGDKLVGVEYDDACDPKQAVAVANKIVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMISPGATNPELTQRGYQHIMRTAGLDSSQGPTAAKYILETVK
                     PQRIAIIHDKQQYGEGLARSVQDGLKAANANVVFFDGITAGEKDFSALIARLKKENID
                     FVYYGGYYPEMGQMLRQARSVGLKTQFMGPEGVGNASLSNIAGDAAEGMLVTMPKRYD
                     QDPANQGIVDALKADKKDPSGPYVWITYAAVQSLATALERTGSDEPLALVKDLKANGA
                     NTVIGPLNWDEKGDLKGFDFGVFQWHADGSSTAAK"
     gene            30393..30776
                     /gene="panM"
                     /locus_tag="OO357_000665"
     CDS             30393..30776
                     /gene="panM"
                     /locus_tag="OO357_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase autocleavage activator
                     PanM"
                     /protein_id="HCR5885718.1"
                     /translation="MKLTIIRLENFSDQDRIDLQKIWPEYSPSSLQVDDNHRIYAARF
                     NERLLAAVRVTLSGTEGALDSLRVREVTRRRGVGQYLLEEVLRNNPGVSCWWMADAGV
                     EDRGVMTAFMQALGFTAQQGGWEKR"
     gene            complement(30975..32078)
                     /gene="livJ"
                     /locus_tag="OO357_000666"
     CDS             complement(30975..32078)
                     /gene="livJ"
                     /locus_tag="OO357_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched chain amino acid ABC transporter
                     substrate-binding protein LivJ"
                     /protein_id="HCR5885719.1"
                     /translation="MNIKGKALLAGCIALAFSNMALAEDIKVAVVGAMSGPVAQYGDQ
                     EFTGAEQAVADINAKGGIKGNKLQIVKYDDACDPKQAVAVANKVVNDGIKYVIGHLCS
                     SSTQPASDIYEDEGILMITPAATAPELTARGYQLILRTTGLDSDQGPTAAKYILEKVK
                     PQRIAIVHDKQQYGEGLARAVQDGLKKGNANVVFFDGITAGEKDFSTLVARLKKENID
                     FVYYGGYHPEMGQILRQARAAGLKTQFMGPEGVANVSLSNIAGESAEGLLVTKPKNYD
                     QVPANKPIVDAIKAKKQDPSGAFVWTTYAALQSLQAGLNQSDDPAEIAKYLKANSVDT
                     VMGPLTWDEKGDLKGFEFGVFDWHANGTATDAK"
     gene            complement(32349..33203)
                     /gene="rpoH"
                     /locus_tag="OO357_000667"
     CDS             complement(32349..33203)
                     /gene="rpoH"
                     /locus_tag="OO357_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HCR5885720.1"
                     /translation="MTDKMQSLALAPVGNLDSYIRAANAWPMLSADEERALAEKLHYH
                     GDLEAAKTLILSHLRFVVHIARNYAGYGLPQADLIQEGNIGLMKAVRRFNPEVGVRLV
                     SFAVHWIKAEIHEYVLRNWRIVKVATTKAQRKLFFNLRKTKQRLGWFNQDEVEMVARE
                     LGVTSKDVREMESRMAAQDMTFDLSSDDDSDSQPMAPVLYLQDKSSNFADGIEDDNWE
                     EQAANRLTDAMHGLDERSQDIIRARWLDEDNKSTLQELADRYGVSAERVRQLEKNAMK
                     KLRAAIEA"
     gene            complement(33448..34506)
                     /gene="ftsX"
                     /locus_tag="OO357_000668"
     CDS             complement(33448..34506)
                     /gene="ftsX"
                     /locus_tag="OO357_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HCR5885721.1"
                     /translation="MNKRDAINHIRQFGGRLDRFRKSVGGSGDGGRNAPKRAKSSPKP
                     VNRKTNVFNEQVRYAFHGALQDLKSKPFATFLTVMVIAISLTLPSVCYMVYKNVNQAA
                     TQYYPSPQITVYLQKTLDDDAAAGVVAQLQAEQGVEKVNYLSREDALGEFRNWSGFGG
                     ALDMLEENPLPAVAVVIPKLDFQGTESLNTLRDRITQINGIDEVRMDDSWFARLAALT
                     GLVGRVSAMIGVLMVAAVFLVIGNSVRLSIFARRDSINVQKLIGATDGFILRPFLYGG
                     ALLGFSGALLSLILSEILVLRLSSAVAEVAQVFGTKFDINGLSFDECLLLLLVCSMIG
                     WVAAWLATVQHLRHFTPE"
     gene            complement(34499..35167)
                     /gene="ftsE"
                     /locus_tag="OO357_000669"
     CDS             complement(34499..35167)
                     /gene="ftsE"
                     /locus_tag="OO357_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HCR5885722.1"
                     /translation="MIRFEHVSKAYLGGRQALQGVTFHMQPGEMAFLTGHSGAGKSTL
                     LKLICGIERPSAGKIWFSGHDITRLKNREVPFLRRQIGMIFQDHHLLMDRTVYDNVAI
                     PLIIAGASGDDIRRRVSAALDKVGLLDKAKNFPIQLSGGEQQRVGIARAVVNKPAVLL
                     ADEPTGNLDDALSEGILRLFEEFNRVGVTVLMATHDINLISRRSYRMLTLSDGHLHGG
                     VGHE"
     gene            complement(35170..36669)
                     /gene="ftsY"
                     /locus_tag="OO357_000670"
     CDS             complement(35170..36669)
                     /gene="ftsY"
                     /locus_tag="OO357_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HCR5885723.1"
                     /translation="MAKEKRRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEP
                     AKASEQAVEEQPQAHTEAEAETFAADVVEVTEQVVESEKAQPEAEVVAQPEPVVEETP
                     EPVAIEREELPLPEDVNAEEVSSEEWQAEAETVEIVEAAEEEEAAKEEITDEELEAQA
                     LAAEAAEEAVMVVPPAEEEQPVAEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFIS
                     LFRGKKIDDDLFEELEEQLLIADVGVETTRKIITNLTEGASRKQLRDAEALYGLLKEE
                     MGEILAKVDEPLNVEDKTPFVILMVGVNGVGKTTTIGKLARQFEQQGKSVMLAAGDTF
                     RAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNK
                     SHLMEELKKIVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKL
                     DGTAKGGVIFSVADQFGIPIRYIGVGERIEDLRPFKADDFIEALFARED"
     gene            36819..37415
                     /gene="rsmD"
                     /locus_tag="OO357_000671"
     CDS             36819..37415
                     /gene="rsmD"
                     /locus_tag="OO357_000671"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase"
                     /protein_id="HCR5885724.1"
                     /translation="MKKPNHSGSGQIRIIGGQWRGRKLPVPDSPGLRPTTDRVRETLF
                     NWLAPVIVDAQCLDCFAGSGALGLEALSRYAAGATLIEMDRVVSQQLIKNLATLKAGN
                     ARVVNSNAMSFLAQKGTPHNIVFVDPPFRRGLLEETINLLEDNGWLADEALIYVESEV
                     ENGLPTVPANWSLHREKVAGQVAYRLYQHEAQGESDAD"
     gene            37405..37674
                     /locus_tag="OO357_000672"
     CDS             37405..37674
                     /locus_tag="OO357_000672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1145 family protein"
                     /protein_id="HCR5885725.1"
                     /translation="MLINIGRLLMLCVWGFLILNLVHPFPRPLNIFVNVALIFTVLMH
                     GMQLALLKSTLPKDGPQMTTAEKVRIFLFGVFELLVWQKKFKVKK"
     gene            complement(37677..38036)
                     /locus_tag="OO357_000673"
     CDS             complement(37677..38036)
                     /locus_tag="OO357_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2500 domain-containing protein"
                     /protein_id="HCR5885726.1"
                     /translation="MSKPPLFFIVIIGLIVVAASFRFMQQRREKADNDMAPLQQKLVV
                     VSNKREKPINDRRSRQQEVTPAGTSMRYEASFKPQSGGMEQTFRLDAQQYHALTVGDK
                     GTLSYKGTRFVSFVGEQ"
     gene            38177..38803
                     /locus_tag="OO357_000674"
     CDS             38177..38803
                     /locus_tag="OO357_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HCR5885727.1"
                     /translation="MLWSFIAVCLSAWLSVDASYRGPTWQRWVFKPLTLLLLLLLAWQ
                     APMFDAISYLVLAGLCASLLGDALTLLPRQRLMYAIGAFFLSHLLYTIYFASQMTLSF
                     FWPLPLVLLVLGALLLAIIWTRLEEYRWPICTFIGMTLVMVWLAGELWFFRPTAPALS
                     AFVGASLLFISNFVWLGSHYRRRFRADNAIAAACYFAGHFLIVRSLYL"
     gene            38877..41075
                     /gene="zntA"
                     /locus_tag="OO357_000675"
     CDS             38877..41075
                     /gene="zntA"
                     /locus_tag="OO357_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase
                     ZntA"
                     /protein_id="HCR5885728.1"
                     /translation="MSTPDNHGKKAPQFAAFKPLTTVQNANDCCCDGACSSSPTLSEN
                     VSGTRYSWKVSGMDCAACARKVENAVRQLAGVNQVQVLFATEKLVVDADNDIRAQVES
                     AVQKAGYSLRDEQAADEPQASRLKENLPLITLIVMMAISWGLEQFNHPFGQLAFIATT
                     LVGLYPIARQALRLIKSGSYFAIETLMSVAAIGALFIGATAEAAMVLLLFLIGERLEG
                     WAASRARQGVSALMALKPETATRLRNGEREEVAINSLRPGDVIEVAAGGRLPADGKLL
                     SPFASFDESALTGESIPVERATGDKVPAGATSVDRLVTLEVLSEPGASAIDRILKLIE
                     EAEERRAPIERFIDRFSRIYTPAIMAVALLVTLVPPLLFAASWQEWIYKGLTLLLIGC
                     PCALVISTPAAITSGLAAAARRGALIKGGAALEQLGRVTQVAFDKTGTLTVGKPRVTA
                     IHPATGISESELLTLAAAVEQGATHPLAQAIVREAQVAELAIPTAESQRALVGSGIEA
                     QVNGERVLICAAGKHPADAFAGLINELESAGQTVVLVVRNDDVLGIIALQDTLRADAT
                     TAISELNALGVKGVILTGDNPRAAAAIAGELGLEFKAGLLPEDKVKAVTKLNQHAPLA
                     MVGDGINDAPAMKAAAIGIAMGSGTDVALETADAALTHNHLRGLVQMIELARATHANI
                     RQNITIALGLKGIFLVTTLLGMTGLWLAVLADTGATVLVTANALRLLRRR"
     gene            complement(41177..41422)
                     /gene="tusA"
                     /locus_tag="OO357_000676"
     CDS             complement(41177..41422)
                     /gene="tusA"
                     /locus_tag="OO357_000676"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA"
                     /protein_id="HCR5885729.1"
                     /translation="MTDLFSSPDHTLDALGLRCPEPVMMVRKTVRNMQPGETLLIIAD
                     DPATTRDIPGFCTFMEHELVAKETDGLPYRYLIRKGG"
     gene            41643..42308
                     /locus_tag="OO357_000677"
     CDS             41643..42308
                     /locus_tag="OO357_000677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine/7-aminomethyl-7-
                     deazaguanine transporter"
                     /protein_id="HCR5885730.1"
                     /translation="MNVFSQTQRYKALFWLSLFHLLVITSSNYLVQLPVSIFGFHTTW
                     GAFSFPFIFLATDLTVRIFGAPLARRIIFAVMIPALLISYVISSLFYMGSWQGFGALA
                     HFNLFVARIATASFMAYALGQILDVHVFNRLRQSRRWWLAPTASTLFGNVSDTLAFFF
                     IAFWRSPDAFMAEHWMEIALVDYCFKVLISIVFFLPMYGVLLNMLLKRLADKSEINAL
                     QAS"
     gene            42381..42938
                     /gene="dcrB"
                     /locus_tag="OO357_000678"
     CDS             42381..42938
                     /gene="dcrB"
                     /locus_tag="OO357_000678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417929.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage sensitivity protein DcrB"
                     /protein_id="HCR5885731.1"
                     /translation="MRNLVKYVGIGLLVMGLAACDDKDTNATAQGSVAESNATGNPVN
                     LLDGKLSFSLPADMTDQSGKLGTQANNMHVWSDATGQKAVIVIMGDDPKEDLAVLAKR
                     LEDQQRSRDPQLQVVTNKAIELKGHKMQQLDSIISAKGQTAYSSVILGNVGNQLLTMQ
                     ITLPADDQQKAQTTAENIINTLVIQ"
     gene            complement(42942..44159)
                     /locus_tag="OO357_000679"
     CDS             complement(42942..44159)
                     /locus_tag="OO357_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417930.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5885732.1"
                     /translation="MPEPVAEPALNGLRLNLRIVSIVMFNFASYLTIGLPLAVLPGYV
                     HDVMGFSAFWAGLVISLQYFATLLSRPHAGRYADLLGPKKIVVFGLCGCFLSGLGYLT
                     AGLTASLPVISLLLLCLGRVILGIGQSFAGTGSTLWGVGVVGSLHIGRVISWNGIVTY
                     GAMAMGAPLGVVFYHWGGLQALALIIMGVALVAILLAIPRPTVKASKGKPLPFRAVLG
                     RVWLYGMALALASAGFGVIATFITLFYDAKGWDGAAFALTLFSCAFVGTRLLFPNGIN
                     RIGGLNVAMICFSVEIIGLLLVGVATMPWMAKIGVLLAGAGFSLVFPALGVVAVKAVP
                     QQNQGAALATYTVFMDLSLGVTGPLAGLVMSWAGVPVIYLAAAGLVAIALLLTWRLKK
                     RPPEHVPEAASSS"
     gene            44293..45342
                     /locus_tag="OO357_000680"
     CDS             44293..45342
                     /locus_tag="OO357_000680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5885733.1"
                     /translation="METPQPDKTGMHILLKLASLVVILAGIHAAADIIVQLLLALFFA
                     IVLNPLVTWFIRRGVQRPVAITIVVVVMLIALTALVGVLAASFNEFISMLPKFNKELT
                     RKLFKLQEMLPFLNLHMSPERMLQRMDSEKVVTFTTALMTGLSGAMASVLLLVMTVVF
                     MLFEVRHVPYKMRFALNNPQIHIAGLHRALKGVSHYLALKTLLSLWTGVIVWLGLALM
                     GVQFALMWAVLAFLLNYVPNIGAVISAVPPMIQVLLFNGVYECILVGALFLVVHMVIG
                     NILEPRMMGHRLGMSTMVVFLSLLIWGWLLGPVGMLLSVPLTSVCKIWMETTKGGSKL
                     AILLGPGRPKSRLPG"
     gene            45397..45984
                     /gene="acpT"
                     /locus_tag="OO357_000681"
     CDS             45397..45984
                     /gene="acpT"
                     /locus_tag="OO357_000681"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase AcpT"
                     /protein_id="HCR5885734.1"
                     /translation="MYRIVLGKVSTLSAAPLPPGLREQAPQGPRRERWLAGRTLLSHT
                     LSPLPEIIYGEQGKPAFAPETPLWFNLSHSGDDIALLLSDEGEVGCDIEVIRPRVNWR
                     WLANAVFSLGEHAEMDAVHPDQQLEMFWRIWTRKEAIVKQRGGSAWQIVSVDSTYHSS
                     LSVSHCQLENLSLAICTPTPFTLTADSVQWIDSVN"
     gene            46095..47669
                     /gene="nikA"
                     /locus_tag="OO357_000682"
     CDS             46095..47669
                     /gene="nikA"
                     /locus_tag="OO357_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter, nickel/metallophore
                     periplasmic binding protein"
                     /protein_id="HCR5885735.1"
                     /translation="MLSTLRRTLFALLACASFIVHAAAPDEITTAWPVNVGPLNPHLY
                     TPNQMFAQSMVYEPLVKYQADGSVIPWLAKSWTHSEDGKTWTFTLRDDVKFSNGEPFD
                     AEAAAENFRAVLDNRQRHAWLELANQIVDVKALNKTELQITLKSAYYPFLQELALPRP
                     FRFIAPSQFKNHETMNGIKAPIGTGPWILQESKLNQYDVFVRNENYWGEKPAIKKITF
                     NVIPDPTTRAVAFETGDIDLLYGNEGLLPLDTFARFSQNPAYHTQLSQPIETVMLALN
                     TAKAPTNELAVREALNYAVNKKSLIDNALYGTQQVADTLFAPSVPYANLGLKPRQYDP
                     QKAKELLEKAGWTLPAGKDIREKNGQPLRIELSFIGTDALSKSMAEIIQADMRQIGAD
                     VSLIGEEESSIYARQRDGRFGMIFHRTWGAPYDPHAFLSSMRVPSHADFQAQQGLADK
                     PLIDKEIGEVLATHDETQRQALYRDILTRLHDEAVYLPISYISMMVVSKPELGNIPYA
                     PIATEIPFEQIKPVKP"
     gene            47669..48613
                     /gene="nikB"
                     /locus_tag="OO357_000683"
     CDS             47669..48613
                     /gene="nikB"
                     /locus_tag="OO357_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikB"
                     /protein_id="HCR5885736.1"
                     /translation="MLRYVLRRFLLLIPMVLAASVIIFLMLRLGTGDPALDYLRLSNL
                     PPTPEMLASTRTMLGLDQPLYVQYGTWLWKALHLDFGISFASQRPVLDDMLNFLPATL
                     ELAGAALVLILLTSVPLGIWAARHRDRLPDFAVRFIAFLGVSMPNFWLAFLLVMAFSV
                     YLQWLPAMGYGSWQHIILPAVSIAFMSLAINARLLRASMLDVAGQRHVTWARLRGLND
                     KQTERRHILRNASLPMITAVGMHIGELIGGTMIIENIFAWPGVGRYAVSAIFNRDYPV
                     IQCFTLMMVVVFVVCNLIVDLLNAALDPRIRRHEGAHA"
     gene            48610..49443
                     /gene="nikC"
                     /locus_tag="OO357_000684"
     CDS             48610..49443
                     /gene="nikC"
                     /locus_tag="OO357_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel ABC transporter permease subunit NikC"
                     /protein_id="HCR5885737.1"
                     /translation="MNFFLSSRWSVRLALIIIALLALIALTSQWWQPYDPQAIDLPSC
                     LLSPDAQHWLGTDHLGRDIFSRLMAATRVSLGSVMACLLLVLTLGLVIGGSAGLIGGH
                     VDQATMRVADMFMTFPTSILSFFMVGVLGTGLTNVIIAIALSHWAWYARMVRSLVISL
                     RQREFVLASRLSGAGHVRVFVDHLAGAVIPSLLVLATLDIGHMMLHVAGMSFLGLGVT
                     APTAEWGVMINDARQYIWTQPLQMFWPGLALFISVMAFNLVGDALRDHLDPHLVTEHA
                     H"
     gene            49443..50207
                     /gene="nikD"
                     /locus_tag="OO357_000685"
     CDS             49443..50207
                     /gene="nikD"
                     /locus_tag="OO357_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikD"
                     /protein_id="HCR5885738.1"
                     /translation="MPQQIELRNITLQAAQPLVHGVSLTLQRGRVLALVGGSGSGKSL
                     TCAATLGILPAGVRQTAGEILADGKPVSPYALRGIKIATIMQNPRSAFNPLHTMHTHA
                     RETCLALGKPADDATLTAAIEAVGLENAARVLKLYPFEMSGGMLQRMMIAMAVLCESP
                     FIIADEPTTDLDVVAQARILDLLESIMQKQAPGMLLVTHDMGVVARLADDVAVMSQGK
                     IVEQGDVETLFNAPKHTVTRSLVSAHLALYGMELAS"
     gene            50204..51010
                     /gene="nikE"
                     /locus_tag="OO357_000686"
     CDS             50204..51010
                     /gene="nikE"
                     /locus_tag="OO357_000686"
                     /EC_number="7.2.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel import ATP-binding protein NikE"
                     /protein_id="HCR5885739.1"
                     /translation="MTLLNVCGLSHHYAHGGFSGKHQHQAVLNNVSLTLKSGETVALL
                     GRSGCGKSTLARLLVGLESPSQGNISWHGESLAKLNRAQRKAFRRDIQMVFQDSISAV
                     NPRKTVREILREPMRHLLSLKKSEQLARASEMLKAVDLDDSVLDKRPPQLSGGQLQLV
                     CLARALAVEPKLLILDEAVSNLDLVLQAGVIRLLKKLQQQFGTACLFITHDLRLVERF
                     CQRVMVMDNGQIAETQAVGDKLTFSSDAGRVLQNAVLPAFPVRRRTSEKV"
     gene            51016..51417
                     /gene="nikR"
                     /locus_tag="OO357_000687"
     CDS             51016..51417
                     /gene="nikR"
                     /locus_tag="OO357_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-responsive transcriptional regulator
                     NikR"
                     /protein_id="HCR5885740.1"
                     /translation="MQRVTITLDDDLLETLDSLSQRRGYNNRSEAIRDILRSALAQEA
                     TQQHGTQGFAVLSYVYEHEKRDLASRIVSTQHHHHDLSVATLHVHINHDDCLEIAVLK
                     GDMGDVQHFADDVIAQRGVRHGHLQCLPKED"
     gene            complement(51537..51896)
                     /locus_tag="OO357_000688"
     CDS             complement(51537..51896)
                     /locus_tag="OO357_000688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HCR5885741.1"
                     /translation="MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSD
                     SIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKTFTLRYPESLSERLNNAAAQQQV
                     SVNTYIIETLNERLNHL"
     gene            complement(51893..52168)
                     /locus_tag="OO357_000689"
     CDS             complement(51893..52168)
                     /locus_tag="OO357_000689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicA family
                     toxin"
                     /protein_id="HCR5885742.1"
                     /translation="MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEI
                     KEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESVCDWLLSIGVKP"
     gene            complement(52227..53351)
                     /locus_tag="OO357_000690"
     CDS             complement(52227..53351)
                     /locus_tag="OO357_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709253.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5885743.1"
                     /translation="MRHLRNIFNLGIKELRSLLGDKAMLTLIVFSFTVSVYSSATVTP
                     GSLNLAPIAIADMDQSQLSNRIVNSFYRPWFLPPEMITADEMDAGLDAGRYTFAINIP
                     PNFQRDVLAGRQPDIQVNVDATRMSQAFTGNGYIQNIINGEVNSFVARYRDNSEPLVS
                     LETRMRFNPNLDPAWFGGVMAIINNITMLAIVLTGSALIREREHGTVEHLLVMPITPF
                     EIMMAKIWSMGLVVLVVSGLSLVLMVKGVLGVPIEGSIPLFMLGVALSLFATTSIGIF
                     MGTIARSMPQLGLLVILVLLPLQMLSGGSTPRESMPQMVQDIMLTMPTTHFVSLAQAI
                     LYRGAGFEIVWPQFLTLMAIGGAFFTIALLRFRKTIGTMA"
     gene            complement(53351..56086)
                     /gene="rbbA"
                     /locus_tag="OO357_000691"
     CDS             complement(53351..56086)
                     /gene="rbbA"
                     /locus_tag="OO357_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated ATPase/putative transporter
                     RbbA"
                     /protein_id="HCR5885744.1"
                     /translation="MTHLELVPVPPVAQLAGVSQHYGKTVALNNITLDIPARCMVGLI
                     GPDGVGKSSLLSLISGARVIEQGNVVVLGGDMHDPKHRRDVCPRIAWMPQGLGKNLYH
                     TLSVYENVDFFARLFGHDKAEREARINELLTSTGLAPFRDRPAGKLSGGMKQKLGLCC
                     ALIHDPELLILDEPTTGVDPLSRAQFWDLIDSIRQRQSNMSVLVATAYMEEAERFDWL
                     VAMNAGEVLATGSAEELRQQTQSATLEEAFINLLPQAQRQAHQAVVIPPYQPENAEIA
                     IEARDLTMRFGSFVAVDHVNFRIPRGEIFGFLGSNGCGKSTTMKMLTGLLPASEGEAW
                     LFGQPVDPKDIDTRRRVGYMSQAFSLYNELTVRQNLELHARLFHIPEAEIPARVAEMS
                     ERFKLNDVEDVLPESLPLGIRQRLSLAVAVIHRPEMLILDEPTSGVDPVARDMFWQLM
                     VDLSRQDKVTIFISTHFMNEAERCDRISLMHAGKVLASGTPQELVEKRGAASLEEAFI
                     AYLQEAAGQSNEAEAPPVVHDTTHAPRQGFSLRRLFSYSRREALELRRDPVRSTLALM
                     GTVILMLIMGYGISMDVENLRFAVLDRDQTVSSQAWTLNLSGSRYFIEQPPLTSYDEL
                     DRRMRAGDITVAIEIPPNFGRDIARGTPVELGVWIDGAMPSRAETVKGYVQAMHQSWL
                     QDVASRQSTPASQSGLMNIETRYRYNPDVKSLPAIVPAVIPLLLMMIPSMLSALSVVR
                     EKELGSIINLYVTPTTRSEFLLGKQLPYIALGMLNFFLLCGLSVFVFGVPHKGSFLTL
                     TLAALLYIIIATGMGLLISTFMKSQIAAIFGTAIITLIPATQFSGMIDPVASLEGPGR
                     WIGEVYPTSHFLTIARGTFSKALDLTDLWQLFIPLLIAIPLVMGLSILLLKKQEG"
     gene            complement(56083..57150)
                     /locus_tag="OO357_000692"
     CDS             complement(56083..57150)
                     /locus_tag="OO357_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5885745.1"
                     /translation="MDKSKRHLAWWVVGALAVAAVVAWWLLRPAGVPEGFAVSNGRIE
                     ATEVDIASKIAGRIDTILVKEGQFVREGEVLAKMDTRVLQEQRLEAIAQIKEAQSAVA
                     AAQALLEQRQSETRAAQSLVNQRQAELDSVAKRHTRSRSLAQRGAISAQQLDDDRAAA
                     ESARAALESAKAQVSASKAAIEAARTNIIQAQTRVEAAQATERRIAADIDDSELKAPR
                     DGRVQYRVAEPGEVLAAGGRVLNMVDLSDVYMTFFLPTEQAGTLKLGGEARLILDAAP
                     DLRIPATISFVASVAQFTPKTVETSDERLKLMFRVKARIPPELLQQHLEYVKTGLPGV
                     AWVRVNEELPWPDDLVVRLPQ"
     gene            complement(57516..59138)
                     /locus_tag="OO357_000693"
     CDS             complement(57516..59138)
                     /locus_tag="OO357_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5885746.1"
                     /translation="MKIGTVAGTNGSTTTIATNDMVQEHVTNFTKELFGYIANGIGDD
                     ISSIARTMLGEVVEKIDDWQIERFQQSIQDDKISFTIQTNHSEKYSMLSGMRAHILRR
                     NNCYQFIVTINSKNYGCPLDNTDINWCSIVYLLNNMTVNDNANDVAVTESYKPIWNWE
                     ISQYNVFDIKFETIIKPQFADRTYFSNCSPVDPTSTRPTYFGDTDGSVGAVLYALFAT
                     GHLGIMAEGENFLSQLLNIEDEVLNVLLRENFNEQLDTNVNTIISILNRRDNVLESLQ
                     PYLVINKDAVTPCTFLGDQTGDRFSNICGDQFIIDLLKRIMSINENVHVLAGNHETNC
                     NGNYMQNFTRMKPLDEDTYDGIKDYPVCFYDPKYKIMANHHGITFDDQRKRYIIGPIT
                     VSIDEMTNALDPVELAEIINKKHHAIINGKKFKTSRAISCRSFNRYFSVSTDYRPKLE
                     ALLACSQMLGINQVVAHNGNGGRERIGETGTVLGLNARDSKHAGRMFSMHNCQINPGA
                     GPEITTPWKSYQHEKNRNGLMPLIRRRTMLQL"
     gene            complement(59400..60989)
                     /locus_tag="OO357_000694"
     CDS             complement(59400..60989)
                     /locus_tag="OO357_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000643692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4049 domain-containing protein"
                     /protein_id="HCR5885747.1"
                     /translation="MKAIDNIFKYIPSNHKDKHSDKVNNHQHHSKVDKTHRSKIVEID
                     KLDNDSQIDNDFGMHIIYFLQHGHWRVNEHSLQMEKIWFYNSEPSIDIQEYNSFADNT
                     TNTFIFTIIPDNNHVIKLSSPITVKIECKDGYYFINFSGDKSDIIYKVNGLSIIPENF
                     FTLLSGNFKADWRWDVSKETFPKDKFDSYVKSVFSKIDFYKQCGVINPQNANTAYFGD
                     TDGRVGAVLYSLLVSGHIGIREKGWSLLCELLKHEDMASSAYIHKNNKLLYNLLNTRD
                     MILNELHQHVFLKDDAITPCIFLGDHTGDRFSTIFGDKYILTLLNSMRNMEGNKDSRI
                     NKNVIVLAGNHEINFNGNYTARLENYKLSAGDTYNLIKTLDVCNYNSETKVLTSHHGI
                     IRNEENQCYCLGALQVPFNQMKDPIDPEELANICNKKHKQHMDDHLLHLIRSNAIAPT
                     PVYENYFNNTTAFRPKPEDIFKCGQTLKKTKQKFGHYGLGVDQHQKIDNYTMGLNSWK
                     IAPNERGDKKGVPGLSCFQPQ"
     gene            61373..62404
                     /locus_tag="OO357_000695"
                     /pseudo
     CDS             61373..62404
                     /locus_tag="OO357_000695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417948.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2776 family protein"
     gene            complement(62491..62967)
                     /locus_tag="OO357_000696"
     CDS             complement(62491..62967)
                     /locus_tag="OO357_000696"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885748.1"
                     /translation="MTNKKMYFILILVFTLLQVCFFALWKARDGSTTSLECTSTLTRN
                     AKTDHSLYYSANLSVILKKDGSGSFTIVGLTDEDTPRKFSHSYFFTYKIDSNGRISGN
                     AKAKVSGLENQIKDENFRLNFLDASLTGKGNARLSKFNNVYIFSIPGLIINTCAPI"
     gene            complement(62960..63724)
                     /locus_tag="OO357_000697"
     CDS             complement(62960..63724)
                     /locus_tag="OO357_000697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HCR5885749.1"
                     /translation="MERRCFFIINDAIIFDPNKCSLSNVNTQDEINFPATAGCILAVL
                     LESNGGAVDRNSIIDSVLERFSFDLSNNTLNQYISLLRKSFKNIGVEENVILTIPKVG
                     FYISENITISKQSYDDSHLQEDVVEKVERKRSIPLFRILISLVVVIVIIEMFAVAFKD
                     TYSITDYPLVKSGKIDTCDLYLSENLNEIGIAKSQIEAAEISKKHLPCVPGANFIYDV
                     NSMHYLLGEGQRYLSRCIKSSNDETYAICSEVLLYD"
     gene            63970..64731
                     /locus_tag="OO357_000698"
                     /pseudo
     CDS             63970..64731
                     /locus_tag="OO357_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709260.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(64838..65338)
                     /locus_tag="OO357_000699"
     CDS             complement(64838..65338)
                     /locus_tag="OO357_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885750.1"
                     /translation="MLAIMLAGTAFASQAGTLVSQGTEASANLTLTKPIVVNNTIQPV
                     KGVYSGTLTAWTPLATGIVGASDGQSHDYAVTFPDDIYAESSTSADAVISGDNNPDHK
                     LKVSLTTLEQDPPSAASEEIGGKRYMMLKNTGTGGAYRVVSHMKEQVVEPDSYTIRTQ
                     AYIYAE"
     gene            complement(65489..>66634)
                     /locus_tag="OO357_000700"
     CDS             complement(65489..>66634)
                     /locus_tag="OO357_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5885751.1"
                     /translation="SITTNRYASGYATLTEAVSAQDERNRKRDKNSHDGSTISLSQPL
                     GNIGNLNFNTTRYNSSRGTGNTRSTSLSYSTVWRGITFSINWAKNDLLTSHKWKVDRK
                     LSVGISVPLSLGDENQIYASSQMSRSGEQGNNYQVSLSGQNSGGVWWDVATNITNAHQ
                     SQPKSTMNIVQVGKNGSYGQFSSHYSSSENMKQLGANLSGGILITRDGLTFGQNVDGT
                     LALIEAPGATGVNVNGWPGLSTDFRGYAILPVQPYRRDDVILDEKTIGKNYDLPQTSQ
                     LVVPTAGAVVPATLAVKSGDKGLVTLKQKEGKPIPFGAVISYSKDTENMAGIVGEDGI
                     AYVSGLSAEGEFNVKWGYSKDQSCIAKYQLPAKKSASGLYQIAATCL"
BASE COUNT        16189 a        17884 c        16345 g        16216 t
ORIGIN      
        1 ccgttctatt tattagtgaa attacaatct caccggatcg atatgccaga tatgatcagc
       61 gtactctttg atagtacggt cagaagagaa gtagcccata ttggcaatgt tcagcatcgc
      121 ttttgcggtc cactcttcct gaagctcgta gagttcgtcg actttatcct gacaatcgac
      181 atagctgcga taatccgcca gtacctggta gtgatcgccg aagttgatca gcgaatcaac
      241 cagatcgcga tagcgacccg gatcttccgg actgaataca ccgctgccga tttgcgtcag
      301 cacctgatgc agctcctcat ctttctcgta gtattcacgc ggtttgtagc cctgacgacg
      361 cagttcttcc acttcttccg ctgtgttacc aaaaataaag atattgtcag caccgacatg
      421 atccagcatc tcgacattcg caccgtccaa cgtaccgata gtcagcgcac cgttaagcgc
      481 aaacttcatg ttactggtgc cggaagcttc cgtccctgcc agcgaaatct gttcagacag
      541 atctgccgcc ggaatgatca actgcgccag gctaacgctg tagttcggga tgaacacgac
      601 tttcagctta tcgccaatct gcggatcgtt gttgatcact ttcgctacgt cattgatcaa
      661 atgaataatg tgcttcgcca tgtaataggc cgaagccgcc ttaccgccaa aaatattcac
      721 gcgcggtacc cacttcgcat ccgggtcggc cttgatgcgg ttatagcggg taatcacatg
      781 caacacattc atcaattgac gtttgtattc gtgaatacgt ttgatttgta catcgaacaa
      841 cgcctttgga ttcaccacca cattcagctg ctgggcgata tactctgcca gacgcttttt
      901 gttctccagc ttcgcctgat gcacagcgtg attaaccatt gggaaatcac agtgttgttg
      961 cagctcatta agcaggctaa ggtcggtgcg ccagttacgg cccaggtgtt cgtccagcac
     1021 ggctgaaagc gatgggttcg ctaccgccag ccagcgacgc ggcgtcacac cgttggtgac
     1081 gttggtgaaa cgacccggga agattttcgc aaagtcggca aacaacgatt gcaccatcag
     1141 attagagtgc agttccgata caccgttaac tttgtggctc acaacaaccg ccagccaggc
     1201 catacgcaca cgacgaccgt tggattcatc aatgatcgac gcccgtccca gcagatcggt
     1261 atcgttcgga tactgttcct gcaaggtttt caggaaatag tcgttgattt caaagatgat
     1321 ctgcaggtga cgcggcagaa ttttacccag catatcaacc ggccaggttt ccagcgcctc
     1381 gctcatcagc gtgtggttag tgtaggagaa gacctgacaa cacacctcaa acgcgtcgtc
     1441 ccagctaaat tggtgctcat cgatcagcag acgcatcatc tcaggaatcg acagtaccgg
     1501 atgggtatca ttgagatgaa tcgcgatttt atccgccagg ttatcgtagg ttttatgcaa
     1561 ctgataatga cggcttaaaa tgtcctgaat ggtcgaggaa accaagaagt attcctgacg
     1621 caggcgcagc tcacgcccgg agtaggtgga gtcatccgga tacagtacgc gagatacgtt
     1681 ctcggagtgg tttttatctt ccactgccgc gaagtagtca ccctggttga atttaccgag
     1741 gttaatttcg ctactggctt gcgcactcca caaacgcagc gtgttggtcg cgtcggtgtc
     1801 gtaaccaggg attatctgat cgtaagcgac tcccagaatc tcttcggttt caatccagcg
     1861 cgttttttta ccttcctgct gaatgcgacc accaaaacgg actttatagc gcgtgttgtg
     1921 gcgtttgaat tcccacgggt taccgtattc cagccagtag tctggcgact ctttctggct
     1981 accgttaacg atgttctgct tgaacatacc gtagtcatag cggatgccgt aaccgcgccc
     2041 cggcaaccct aacgtcgcca gagaatcaag gaagcaagcc gccagacgtc ccaggccacc
     2101 gttaccgagg cctgggtcat tttcttcatc aatcagctct tcgagattta accccatcgc
     2161 ttccagtgcg ccctgtacat cttcgtaaat tcctagcgac aacatggcgt tggagagcgt
     2221 acggccaatc aaaaactcca tcgacaggta gtaaacctga cgagtttctt gcgacaactg
     2281 ggcacggttt gaacgtaacc agcgctccac gagacgatcg cgcacagcaa ataacgttgc
     2341 gttcagccat tcatgtttat tggcgacgac cgggtccttt ccaatcgtaa acatcagctt
     2401 gtaagcgata gagtgcttaa gagcttctac gctaagcgtg ggcgatgaat atgtaaacgg
     2461 agcattcata taggcgtttc ctgaaaacta tttcaagcga tagtaaagct cacggtacga
     2521 cttcgccgcg acctgccagc taaaatccat tgccatagcc tgacgttgca caaaccgcca
     2581 cagtgaagga cgggaccaca gtacaaaagc acgccgaata gcccgtaaca gcgaccaggc
     2641 attactatct tcaaagacaa acccactggc gacgccatct gcaaggttct caagagaaca
     2701 gtcagaaacc gtatcagcaa gcccaccggt gcgccgcact aacggcagcg taccgtactt
     2761 caatccataa agttgcgtta agccgcacgg ttcaaaacgg ctgggcacca gaatgacgtc
     2821 cgcgccgccc ataatgcgat gcgaaaatgc ttcgtgatag ccaatctgaa cgcccacctg
     2881 accggggtat tccgctgccg ccgcaaggaa accttcctgc agcaccggat cgcccgcgcc
     2941 gagtagcgcc agctgcccgc cctgctccag aagccccggt aaggcttcca gcaccaggtc
     3001 gagacctttc tggctggtca gacggctcac cactgcaaaa agcggcactt tatcgtcaac
     3061 cttaagcccc attgcgattt gtaactggct cttattttcc gctttatctt ccaacgtatc
     3121 gcgggtgtaa cgcgaggcca acagtaagtc cgtctctgga ctccagattt tctcgtccac
     3181 gccgttcagt acgcctgaaa gacgcccttc acggtgacgc tgttgcaaca gaccttccat
     3241 accgtaggca aactgcggtt cggtgatctc gcgagcgtag gttggactga ccgccgtaat
     3301 gtgatcggca tagtacagac cggccttcag gaaagagatt tgtccgttga attccagccc
     3361 atgaatatta aagaatgacc atggcaattg gatgtcattc atgtgatgtg cataaaacat
     3421 gccttgatag gccaggttgt gcacagtaaa caccgacttc gcaggacgcc cgcgcgccgc
     3481 cagatacgca ggcgcaagac ctgcatgcca gtcgtgcaca tgcaccacat caggacgcca
     3541 gaatgggtca agcccgctgg ccatttctgc cccaacccac cccagcagcg caaaacgcaa
     3601 tacgttgtcg gtataggcaa ataagttggt atcgtgatac gggcttcccg gacgatcata
     3661 gagatgcggc gcgtcaatca ggtatatgcc aaccccgttg taatgaccga acaacagcgt
     3721 gatatgcccg gcgaaagtat cacgacggga tactacctgc gcatcggtca cgccacggcg
     3781 aatatcggga aatgcaggca acagtacgcg agcgtcaacg ccgtctgcga tttgtgctgc
     3841 gggtaatgcc ccaataacat cagccagacc gccggtttta agcagcggga acatctctga
     3901 acatacatgt aaaacctgca ttatcgctcc tgtttatgcc ctaacttccg tagcatttcg
     3961 cgcgttacca gcacgatgcc ttcttctgaa cgatagaaac gacgtgcatc ttcctctgcg
     4021 ttttcaccaa tcaccatgcc ttccgggata acacaagcac gatcgatgac gcagcggcgc
     4081 agacggcacg agcgacctac ccatacttcc ggtaacaata cggcggaatc aatgttgcag
     4141 aatgaattca cgcgaacgcg cgagaacaga acggactgca ccaccaccga accggagatc
     4201 acacaaccgc cggataccag tgagttaagg gtcatcccgt ggctaccgga gcgatcctgc
     4261 acgaatttcg ctggcggtaa tgattcattg taggtgcgaa ttggccaatt gcgatcgtac
     4321 atatccagtt ccggcaccac agaggccaga tcgagattcg ctttccagta agcttccagc
     4381 gtacccacat cgcgccagta cggctcggca tccgggtcgg attgtacgca agagagcggg
     4441 aacgggtgcg cataggccag accggcttcg gtgatcttgg gaatcaaatc tttgccaaag
     4501 tcgtggctgg agttctcatc acgatcgtct tcttccagca gttcatacag atagtcggcg
     4561 tcaaagacgt agatacccat actcgccaga gatttgctcg gatcgttcgg cattgacggt
     4621 gggttagcag gtttttcaac gaattcgata attttatcgt tctcatcaac cgccataacg
     4681 ccaaatgcgg aggcttcttc aatcggtact ggcatacaag caacggtgca acgtgcgcct
     4741 ttttcgacgt gatcgataag catacgcgag tagtcttgct tgtagatatg gtcgcccgcc
     4801 aggatcacca cgtattccgc tttataacgg cggataatgt cgaggttttg ggtgaccgca
     4861 tctgcggtgc cgcgatacca gttttcccct ttcattctct gctgtgctgg cagcagatcg
     4921 acaaactcgt tcatttcttc attgaagaat gaccagccgc gctgaatgtg ctgcaccaga
     4981 gtgtgggact ggtactgggt gatcacgccc atacgacgga tcccagagtt gatgcagtta
     5041 gacagcgcaa agtcaataat gcggaactta ccgccgaagt gtacggccgg ttttgctcgc
     5101 ttattggtta aatccttcag gcgggtacca cgtcctcccg ccagtatcag ggcaacagat
     5161 ttcaatggca gctggcgcgc caacattaag tgatcgttct tctctaaact aaccatgact
     5221 aactcctttt ttatcatctc tggaacacac acaatccgtg tgcaggtccc tgccagacaa
     5281 ccgtaatcac tgggttatcc tctccagcga atgggggaat ggcgtgccac tccccagcag
     5341 gtaaaacaat ctctgttacc tcaagcgtgg cgttaattgc gatcaaaaag cgatccgaga
     5401 gcagaatttg cagctgtttc ggcccgtttt gccactcatc cgtgcttaaa ggttgagcat
     5461 atcgatttag ccaacggaca ttgccgtcac cttcttccca ccagcgattc tccaccaaag
     5521 cgggaatgcg cttgcgcaga tggattaacg cggcggtaaa tgcggttaaa ccactgcttg
     5581 cctgcgacca gtccaaccag tttaattggt tatcctgaca gtaggcattg ttattgccat
     5641 gctggctgtg accatgttcg tcaccggcca gtaacatcgg cgtgccctgg gagagcaaca
     5701 acgttgttaa cagggcatga atgctgtcgc gccgccgttc aaccaggtca agagtaccgc
     5761 ctaacccttc tttaccatga ttgttactgt aattgttgtt ggtcccgtcg cgattttctt
     5821 ctccgtttgc ttcattgtgt ttatggttga agcaaacgca gtcgcgaagc gtaaaaccgt
     5881 catgcgcggt gacgagatta atcgcggcac tcggcagacg accattacgt ttaaaaacat
     5941 cgctggaggc agcaaaacgc ccggcaaacg cccccagagg caaatcataa tgtagccaga
     6001 aacgacgggc agcatcgcgg aaatgatcgt tccactcggc aaacagcggc gggaaatttc
     6061 ccacctgata accactagga gcgatatccc acggttcagc aattaacttc acctgcgaga
     6121 gcaccgggca gttctggata gcggtaaaca acggcgcatc ctgacggaac tctggcgtac
     6181 ggcccatgac tgccgccaga tcaaagcgga aaccatcgac gtggcaggtt tctacccaat
     6241 aacgcaggca ggcgctggca taatccacca ccgccggatg actcaaattg agcgtgttgc
     6301 cgcaaccggt ccagttgtga taatcgccgt cttctcttat ccaataatag ctacggttat
     6361 cgatcccacg cagcgagaat aacgggccgt cgaggtccag ttccgcacta tggttgagca
     6421 cgatatcaag aatgacttcg atacccgctt tatgcagtgc tttgattgca tcgcgaaact
     6481 catccagcgc cgtttctggc gagcaggcat acgccggatg cagcgcaaac atcgccaccg
     6541 ggttgtaacc ccagtagtta cttagcccca tgcgttgcag acgtggttca ctggcaaact
     6601 gcgccactgg cagcagttcc agcgcggtaa tgcccaattg tttcaaatag ttgatcatca
     6661 ccggatgccc gagggcttta taagtgccac ggatctcgac cgggatctcc gggtgcaagt
     6721 acgttaatcc tttgacatgg gcttcataaa tgatggtgct gccccacggc gtgcgcggcg
     6781 gggcgtcatc ttcccagtca tagtgatcaa ccaccactac gcatttcggc gcaatggcgg
     6841 cgttgtcgcg atagtcaggt tcattatgac cggcgtgcag cagcgggtta tctttaaact
     6901 ccccgtcaat ttgccgcgcg caaggatcaa tcaacaactt cgccgggtta aagcgatgcc
     6961 cctcggcggg ttgccagggg ccatgaacgc gataaccata acgcaaaccc gggcgcgcat
     7021 ccggcagata accgtgccaa atgtcgccac tgttccctgg caagtcatag cgatgttcct
     7081 ggccattggc gtcaaagaca cacagttcta cccgctcggc atgagcggag aaaagtgtga
     7141 agttgacgcc ctgaccgtcg taatgcgcgc cgaggggagc gggtttgcca atggcgagtt
     7201 gtgtcattct gcctcccgaa ccagccagat agtggccagc ggtggtagcg tcaggcttag
     7261 tgaatgctga cgaccgtggc tggcaatctc atcgctgtgt accgtgccgc cattgcctgc
     7321 attactgccg tgatagtgta tggaatcggt attgaggatt tcacgccatt tacctggctg
     7381 attaatgccg aagcgataat catgacgcgg caccggcgta aagttactgg caacgataat
     7441 ttcgttaccc tctttatcgc gacgcacaaa gatcagcacc gagcgttctt tgtcatccac
     7501 caccagccat tcaaagccgt acgggtcaaa atccagttca tgcattgctt tatggtggcg
     7561 gtaggtgagg ttcagatcgc gcaccagacg ctggacaccg tggtgccagt tatcgccgcc
     7621 ttccaacaga tgccagtcga ggctggcgtc atggttccac tcgcggccct gggcaaattc
     7681 gttccccatg aacagcagtt tcttgcccgg aaatgcccac atccagccat agtaagcgcg
     7741 caggttagcg aatttctgcc atgcgtcgcc cggcatgcgg tcgagaatcg attttttacc
     7801 gtggaccact tcatcatgcg acaacggcag gacgaagttt tcagtgtagt tgtagagcat
     7861 cccgaaggtc agtttatcgt gatgatactg acgataaatc gggtcgagct tcatgtagtc
     7921 cagggtgtca tgcatccagc cgaggttcca cttgtaccag aagcccagac cgcccatatc
     7981 ctgcggacga gaaacgccag ggaaatcggt agactcctca gccattgtca ccgcaccgga
     8041 aacctgctca ccaagaatac ggttggtatt acgcaagaat tcaatcgctt caagattctc
     8101 gcgcccgcca aattcgttcg ggatccactc cccctcttta cggctgtagt cacgataaat
     8161 catcgacgcc accgcatcga cgcgcagcgc atcaatacca aaacgttcaa tccagtaaag
     8221 cgcgttaccg acgaggaagt tactgacttc acggcgacca tagttgtaga tcagcgtgtt
     8281 ccagtcctga tgatagcctt cacgcggatc gctgtgttca tacaagttcg tgccatcaaa
     8341 ttcggcaagc gcaaagtcat cagtcgggaa gtggcctggc acccagtcga gaatcacgtt
     8401 cagaccagct gcgtgtgcgg catcaatgaa ataacggaag tcgtcgcgag taccaaaacg
     8461 gcgggttggt gcatacaggc cggttggctg ataaccccaa ctgccatcga agggatgctc
     8521 gttaatgggc agtagttcga ggtgggtaaa gcccatccat ttagcataag gcaccagttg
     8581 atcggccagc tcgcggtagc tcaaccagaa attgttgtcg gtgtggcgac gccaggaacc
     8641 caggtgaact tcataaatag agattggcgc atcaaactga ttcgcttttt tgcgctcttc
     8701 agtctgtaca accttttccg gcagcccgca aataagagac gcggtttccg ggcgcatttg
     8761 cgcttcgaag gcataagggt cggacttcag acgcaagttg ccattggcat caatcatctc
     8821 gtatttatag agctgaccgt tatgcgcccc agggataaac agttcccaga tgccgctctc
     8881 tttacgcagg cgcatcgggt gacggcgacc gtcccagtag ttgaattgcc caaccaccga
     8941 gacccgacgg gcgtttggag cccagacaga gaaacgcgta cctgtgacgc catccatagt
     9001 atctgcatgc gcgcctaagg tttcatacgg gcgcaggtga gtaccttcag ataatagcca
     9061 ggcatccatt tcctggatta gcggaccaaa acggtaagga tcatcaatca ggttttgctg
     9121 accatgccag acaacagcca actgatagcg gaaaaaattc ttacgtcgcg gaatgacgcc
     9181 gctaaagaat ccccgtgagt cgagacactc cagttttgcg agtttgcgcc cggttttcgg
     9241 ttcaatcacc cacacatcgg tagcgtcggg taaaagggca cggacttcca gtcccgcggt
     9301 ggttttatgc attcccagta cggaaaaagg atccgcaaaa tggcctgcaa ttagcgcgtt
     9361 aatcacgtct ctatcgatac gatcggacat gcttgtcttc ctgttttatt gtgtcacccc
     9421 atccaattgg atttttgact tctggttgtg acattttttt gacctgaacg gcgcagcact
     9481 ctgtgcatcc tctctgcgtc gtcctcactt caggtaaggc tgtgaatact catgtattca
     9541 gccaccctta aagaatagcc aatgctctat ttaactcccg gtaaatcatg aaacatctgc
     9601 gcttactcct gtattacgca ctaacagggg cggcatcgcg ccccagattt aatgaataaa
     9661 gattacgcca gttgacgaag catccgacgc agcggctccg cggcccccca cagcagctgg
     9721 tcgcccacgg taaaggctga caggaactct ggtcccatat tcagcttacg caggcggcct
     9781 accggcgtgg tcagcgtgcc ggtaacggca gctggggtta gctcacgcat agtgatttcc
     9841 cgatcgttcg gaacgacttt cgcccacgga ttgtgcgcag ccagcagttc ttcgacggtc
     9901 ggaatagaca catctttttt caatttaata gtgaatgcct ggctgtggca gcgcaatgcc
     9961 ccgacacgca cacataaacc atctaccgga attacggaag atgtgttgag gatcttgttg
    10021 gtttccgcct gccctttcca ctcttcgcgg ctctgaccgt tatcgagctg tttgtcgatc
    10081 cacggaatca ggctacccgc cagcggcacg ccaaagttat ccaccggcag ctcaccgcta
    10141 cgggttaagg ttgtgacttt gcgttcgata tcgagaatag cagaggacgg ggtcgcgagt
    10201 tcatctgcca catggccata cagatggccc atctgggtta ataactcacg catatgtcgc
    10261 gcaccaccgc cggaagcggc ttggtaggtt gcaacggaca cccaatcaac aagatcattg
    10321 gcgaataaac cacccaacga catcaacatc aggcttacgg tacagttacc gccaacaaaa
    10381 gtcctgatgc cattatttaa tccgtcggta atgacgtcct gattgacggg gtcaagaatg
    10441 atgatggcgt catctttcat gcgcagagac gatgctgcgt caatccagta accttgccat
    10501 ccgctttcac gaagctttgg atagatttcg ttggtataat cgccgccctg acaggtcaca
    10561 atgatatcga gggcctttag cgcctctaga tcaaaggcat cctgaagtgt gccagtggtt
    10621 ccgccaaaag acggcgcagc ctggccaagc tgagaagtag aaaagaagac agggcgaatg
    10681 gcgtcgaagt cgcgctcttc aaccatgcgt tgcatgagaa cggagccgac cataccgcgc
    10741 cagccgataa aaccaacatt tttcataagc gttttttcct gcaaagatgt gtgctgtata
    10801 aatgtgccgg tctcctcttg gcacatcttt caccatacaa aaagctgcca aagtcgcaag
    10861 tgaaattaat caatgatagc gaagccatca gtaatgcgac ttatcctgct ttgttagcac
    10921 gcagaaagtc cgcggcaatt atcagggaat ttgagttatg aatgaaatca tttctgcagc
    10981 agttttattg atcctgatta tggatccgct cggaaacctg cctattttca tgtccgtact
    11041 gaaacatact gaaccgaaaa gacggcgggc aatcatggtg cgagagttgc ttattgctct
    11101 cctggtgatg ctggtgttcc tgtttgcggg tgagaaaatt ctggcatttc tcagcctgcg
    11161 agcagaaacc gtctccattt ctggcggcat cattctgttt ctaatcgcca ttaagatgat
    11221 tttccccagc gcttcaggaa atagcagcgg gcttccggca ggtgaagagc catttatcgt
    11281 gccgttggca attccgttag tcgccgggcc gactattctc gccacgctga tgttgttgtc
    11341 tcatcagtac ccgaatcaga tgggacatct ggtgattgct ctgctgctgg cctggggcgg
    11401 cacctttgtc atcctgctac agtcttcgct atttttacgt ctgctgggcg agaaaggggt
    11461 gaacgcactt gaacgcctga tgggattgat tctggtgatg atggcaactc agatgttcct
    11521 cgacggcatt cgaatgtgga tgaaggggta acgaggagag tttttcgaaa agagcccggt
    11581 agcgtcacaa ctaccgggcg aacaaactta actcaacagc tggaacgcaa tcatcccaac
    11641 gatggcacca acagtgccga ggatggtttc catcatggtc caggttttca gcgtttcggc
    11701 ttcggtcgcg ccggtaaatt taccgaacaa ccagaaacca gcgtcgttaa cgtggctgac
    11761 aacaatcgaa ccaccagcga tacaaatcga cagtgccgcc atttgcgcac cagagtagtt
    11821 cagttgttca atgactggca tcaccagtcc caccgccgtt aaacaggcta cggtggcagc
    11881 cccctgaatg atgcgcactg cagcagccag cacgaagcag gtgatagcaa tcggcaggcc
    11941 catgccggtt aacgcttcgc ccagtgccgg acctacgcca gagtcaacca gcacctgttt
    12001 gaacacgccg cctgcaccaa tcaccagcag aatgatcccc gccggttgca gcgcgtgacc
    12061 gcaaatctcc atcactttgt cttttggcat gccctgacgc attgccaggc cataaatcgc
    12121 taccagacaa gcaaccagaa tcgcggtaaa cggatgacca ataaactcga accattcgta
    12181 agcggtagag ccttctggca caaaacgcgc ggcaatggtt ttcagcccca ccagcaccag
    12241 cggcaacagg atcagcgaca ggctgaatcc gaaagatggc attttacctt cgccgagatg
    12301 cggttcgctg atgtcgtcag gaatatgcag ctcaacgtaa cggctgatga agttacccca
    12361 cagcggcccg gcaataatca ttcccggaat tgccgcacac aggccaatca ggatcatcca
    12421 gccaaagtcg gcattcatct gcgatgccag cagcattggc gctggcccag gcaccaggaa
    12481 cgctgccgcg gccgccacgc ctgcgaataa tgggattacc agcttcacca ggttcgtacc
    12541 ggtgtggcgc gccattgaga aagcaacgct aatcagcaga acaatcgcca cttcaaagaa
    12601 cagcggtagc gcacagacca gccccgcaag gccgatggca taatgcgcgc ggctgtgacc
    12661 gaaggatttg agcattttga cggcaatctg atcgactgcg ccggtttcat ataagatctt
    12721 gccaaacata gctcccaggg cgacaaccac cgccaggaag ccgagggtgc ctcccatccc
    12781 tttttccatc gtcgctgcga ttttatcgag cggcataccg gaaaaaaggc cagcccccat
    12841 ggacaccacc atcaaagcca ggaaagcgtg catacgcgcc ttcatgacta aaaacagcag
    12901 cagtaaaaca gaccctactg ctgttaaaac aagcgttaat gtagtcacta cttatttgcc
    12961 ttttttaata acctcaatgg tgcttgccac aacaccttcc agcggttgat cgatatccac
    13021 caccagtaca tcggtttcgt ccgcacccgg ctcctgcagc gtttcaaact gcgtcaccaa
    13081 catttgggtt ttaaagaaat ggcctttgcg cgctttcagg cgactttcaa tcacatcaaa
    13141 atcgcctttc aggtagatga aagagagatt cggattacct tcacgcagca agtcgcgata
    13201 gtgctttttc agtgcagaac aggcgatcag cgacacttta ttggtgcgct gcatagcaaa
    13261 cgcggagtcg ttcagcgcct gcaaccaggg tttgcgatcg tcgtcattca gcggttcgcc
    13321 agacgccatt ttttcgatat tgcgccgtgg atggaggaaa tcgccatcaa gaaacgcggc
    13381 atgaagttga tgcgccactt cactggcgac cgcagatttg ccgctgcccg atacgcccat
    13441 caagacgtaa atgtggtgat catggttagt cgtgctcaaa gtggtgcccc cacaatacaa
    13501 gaattaaaaa tgttacgggt aactgttatc ggcaacattg tccagccgga caatatcaga
    13561 agcaatatcc atccgtgcct taagtgtata agtgtgagct acttcaaatt tgtgggctta
    13621 aatagatccg cccggtgaca aggtgaaacc taaatctaac attttcggtg tcacagattc
    13681 gccacgaata cgcgccagca ggcgttcagc gccaatactg cccatccgct cacgcggcgt
    13741 cagcacgctc gcaagtcgtg gctccatcac ctgaccaatg tcatgaccgt ggaaaccggc
    13801 aatcgccata tcgtcaggaa cttttaaccc cagacgctga cattcaaacg ccgcgccgac
    13861 cgccagatca tcattagtac agaacacgcc atccagctgc ggatattccc gccgcgcctg
    13921 gcgaatcagt tcaataccgg aagagtaaga agaagattgc tcaaccatca cgctatatgg
    13981 caccaggcct gcatccagca tcgcctgttc gtatcccttc tgtttgatga tagtacgttc
    14041 gtcgagacgt gcgccgagat aggcaatgtg gcgatgccca cgagcaataa tggcggtagt
    14101 catctggcgt gccgcttcaa agttatcaaa accgacggcg atatcgaggc acggcgactg
    14161 gctgtccatc aactccacca ccgggatccc cgccacttca atcatcttta aggtgcgcgg
    14221 cgtgtgggta cgttcggtga ggatcaggcc gtcgatattc caggagagca tggattcgag
    14281 acgttcttgc tccatttccg gtttataacc gtagtgcgcc agcatggtct gataaccgtg
    14341 cgcgtcggtg acgctttcga ttccgcgtaa tacttccgcg aaaacctggt tggtgagaga
    14401 aggtaacagg acgccaatcg cccggctggt agcgttagag aggatatcgg gcgcacgatt
    14461 gggaatatag cccagttcat caagagcggc ggcaatcttg ccgcgtagag cgacggaaac
    14521 ctgctccggg ttgcgtaaaa aacggctgac cgtcattttg gtcacgccta cacggtcagc
    14581 cacatcctga agtacgggtc ttttcttttt catcgtcctg aaggtacaaa agagatagat
    14641 tctcttagtt taacacggac gcatcagaac cttcccgggg aaaacagtgc ttgtttacaa
    14701 ttattagata tacactaaat caattgcatc gcattgtgct aacgacgcgg caaaaccccg
    14761 gaagcgtgca tcagcacgcg accggagcga gcgaggaagc ctgcaaaaag gcaggcttca
    14821 aagatgccgt tgagttttaa accggcggca gatcgaacag taacacttcg ctgtcgctat
    14881 ccgcatggat ggagattgcc tgctcatccc agattgccag accatcgctg gtcgaggctt
    14941 tcacgccgtt aatggtaaca ttgcctttta ccacctgaat ccagacgcgg cgttcagcgg
    15001 caatctgatg caccgactgc tcatctttca gcaacgccca acgatacagt tccatatcct
    15061 gatgcacttt cagcgaacca tctcgcgcat ccggcgagag caccagctgt ttgccctgca
    15121 cggcatcgaa gcgacgctgt tcataacgcg gcgtaatgcc attttcttca ggcatgatcc
    15181 agatctgata cagatgcaga cgctcggtgc tgcttgggtt gtactctgag tgacgaatac
    15241 ccgtacccgc actcataatc tggaactcac ccgccggaac ctgctcttta ttgcccatgc
    15301 tgtcctgatg ctcaacagta ccttccagca cgtaggtcaa aatttccata tctttatgcg
    15361 ggtgagtgcc gaagccctgc cctacttcaa tcacgtcgtc gttaatcacg cgcagcgcgg
    15421 agaagcccat aaagttcgga tcgtaatagt tggcacaaga gaaagtatgc caggagtcca
    15481 gccagccatg atttgcatga ccacgttcat ttgctttgcg taagtagatc atttgtattc
    15541 gccccctgaa tgatttcgat gggcttagtg tggacccgat ctgcctggga tgatagaggg
    15601 tgaaaattgc cccctctgtt caaaaaaatt gaactattcg aggggccatc aaacttaatt
    15661 cgcgagagtc actgtggagg gagaggcttg ctcaaatccg cgttcaagga tttccagatt
    15721 ggtaagaact tcagattcct tgacgtaatt tggcgcaccg tgggtgatgg tttgatacaa
    15781 cgcatcataa acacgcccgt aatcgcccat ctccggcttc atctcttctc tgaccgtcac
    15841 accctcgtca ttgacatact ccagcacacc gaccgaatca tccgctgcga atcccggttc
    15901 gcccggcata atattagcct tcaggctggt ttcctgctgg tcgataccgt atttaataaa
    15961 cgaacctttc ttaccgtgaa cgataaattt cggataatcg attttcacca gatggctggt
    16021 tttgacgatg gctttcaggt cgccataaaa cagttgcgct tcaaaggtgt cgtcaggatt
    16081 ggctttatta cgcaggctgc ggatgtcata agcgacgtga tccgggcgac cgaacagaga
    16141 aataatctgg tccatcgtat gcacaccaag gccatagaac gcgccatcct gcggcagtcc
    16201 aggtttggtt tctgccaccg ggcggtaata gtcaaaatgg ctttccactt cgacaatctc
    16261 tcccagcttg ccgctttcaa tcgctttttt cgccgtcagg aagcaggaat caaagcgacg
    16321 attctgatat ggcgtgacgg tcagcccttt gcttttcgcc agtgcaaaca gctctttcgc
    16381 ctgcgcaagt gtcggagtga acgttttttc gaccagcaca tttttcccgg cttccagcgc
    16441 gcgcttcgcg tactcgaagt ggctgtccgc atgggtgcag acaacaacca acttaacatc
    16501 gggatcgttt agcacttcgt cgagatcgct ggtgaaatgg atatgagagt aaatgggagc
    16561 ctgttcttcc ggcttcgcat ggcgacgaaa aatatgcgcg acatgccagc tatccttgcg
    16621 gttaagtaca tacggcagat ggtaacgggt ggtgcttttg ccgaagccaa taaaggcgca
    16681 gttgatgacc atgatttcgt cctttttaag gtggttattt acaccttagc gcaaagcgga
    16741 cgtggttcct actggagtgc gcataacgaa cacaagcact cccgtggata aattgagaac
    16801 gaaagatcaa aaaaaaagcc agcacccggc tggctaagta atactggaag caatgtgagc
    16861 aatgtcgtgc tttcaggttc tccgcgaggg tcttcctgat cgcgatacaa taataatcat
    16921 tctcattcgc acttgtccaa cactttttgc aaaaaaatgc atttgactcg catttgaaag
    16981 tcaatgatgt tgaaagggac atttacccca aagaggacaa aggaatgagt gagatagtaa
    17041 tacgccacgc agaaacacgg gattacgagg ccatcaggca gattcacgcc cagccggagg
    17101 tgtattgcaa cacactacag gtgcctcatc cttccgatca tatgtggcag gagcgactcg
    17161 ccgatcgtcc cggcatcaag caactcgtcg cctgtattga tggagacgtc gtgggccatc
    17221 tcaccattga cgtgcaacag cgcccacgcc gcagtcatgt tgccgatttt ggtatctgtg
    17281 tcgactctcg ttggaagaac cgcggcgtcg ccagcgccct gatgcgagag atgattgaaa
    17341 tgtgcgacaa ctggttacgg gtagatcgca ttgaactaac cgtgtttgtc gataacgcgc
    17401 cagcaattaa ggtctataaa aaattcggct ttgagattga agggaccggt aagaagtacg
    17461 cattgcgtaa tggtgaatat gtcgatgcgt attatatggc gcgggtgaaa tagagaattt
    17521 gcaatctaag tttgtcattt aagccctcac ctaccgggag ggcttaagat attaataccc
    17581 cgccgttaaa tcatccaccg agcgcgggtc ggatgcgccg tacaactcac cgtccggccc
    17641 aaccataatg ctttgtgtgc tgcccatcgc ctctttcagc gccactttct gaccttttgc
    17701 ttccagcagc ttgagcgtat ccgggctaaa ccctttttcg acacgcagct cgtccggcaa
    17761 ccactgatgg tggaaacgcg gcgcattggt cgcttcggcg acgttcatgc cataatcgat
    17821 gctattcacc accatttgca gcaccgtggt gataatccgg ctaccgcctg ggctaccggt
    17881 aaccagccag gttttaccgt ctttcaccac aatggtcggc gacatcgacg acagcgggcg
    17941 tttgttcggc ccgacggcgt tggcatcacc gcccaccagc ccgtaaacgt tcggtacgcc
    18001 cggtttggcg gagaaatcat ccatctggtt attaagcaga ataccgctct cacccgcgac
    18061 aatacccgta ccgaaggtgg tgttcagcgt ataggtcacc gccaccgcgt taccgtcttt
    18121 atccactaca gagtaatggg tagtttgatt actctcataa ggcgcaagct tgccagggcg
    18181 aatctcactg gacggcttcg cttttttgat atcaatttga tcggcaatgg atttggcata
    18241 ggctttattg gtcagtgcct gccacgggac tttgacaaaa tccgggtcgc caagatattc
    18301 cgagcggtcg gcgtaggcgt atttctccgc ttctgccatg atttgcatcg catcggcgct
    18361 gccaaagccg tatttcttca tatcgaagtt ttccagaata ttgaggattt gtacgatatg
    18421 gatcccgccg gaggatggcg gtggcatgga gtaaacctga tacccgcgat aatcgccgct
    18481 tatcggagtg cgttcgaccg ctttataggc cgctaaatct tctttagtga tcaagccacc
    18541 gtttttctgc atctcctggg cgatctgttc cgcaatcgtg cctttataga attcgtccgg
    18601 gccgttttca gcaatcatct ccaggctctt tgccaggttc gcctgcacca gcgtgtcgcc
    18661 ctttttcagc ggctcgccct ctttccagaa gatagcttta ctgttttcgt gattcggcaa
    18721 cacttcgcta ccgtaggttt tgagatcgtc agccagcgcg tcgttaacga taaaaccatc
    18781 gcgtgccagt ttaaacgcgg gctgcacgac tttgttcagc ggcatggtgc cgtatttatc
    18841 cagcgccagc gagaaacctg ctaccgtacc cggtgtgccg gaagccagat gcgaagtgag
    18901 tgattttttg ctgtccgggt tgccctgatc atcgaggaac atatcgcggg tcgctttggc
    18961 gggtgccatt tcgcggaaat cgatagccgt ggtattgcca tttttcgagc ggattaacat
    19021 aaaaccacca ccgcccagat tccctgcctg cggatgcgtt accgccagcg cgtagcccac
    19081 cgccacggcg gcatcaacgg cattcccgcc ctccttgagg atatccaccc ccacctgagt
    19141 ggcagtggcg tccacagacg ctaccattcc ctgtttcgcg cgtaccggat ggaagacatc
    19201 ttcctccaca ccatacgaga cgggcggcgc aggaggcgcg gcggcggcgc taaaacaact
    19261 tcctgagagc agagcagcaa tggccacccg gcgtaaaaac gtcggtttta tcatcgttat
    19321 tctccagaga ttaaggggca accccagcta agcctggtat ataactctga attaatcatc
    19381 gttttgtcgg gaagcgagta aacttaaagg atatcttcag aggaggatac gcgatgaaac
    19441 gacttctgat tcttacggca ctcctgccgt ttgtcggctt tgcacagccc attaatactc
    19501 tgaacaaccc taaccagccg gggtatcaga tacccagcca gcagcggatg caaacccaga
    19561 tgcagactca gcaaatccag caaaaaggga tgctgaatca gcaactgaaa acgcaaactc
    19621 agttgcaaca gcagcattta gaaaaccaga taaacaataa ttctcagcgg gtgttgcagt
    19681 cacagccggg ggagcgaaat cccgcccggc agcaaatgct gcccaacacc aacggcggga
    19741 tgttaaacag caaccgtaat ccggatagtt cgttgaatca gcagcatatg ctgccggaga
    19801 ggagaaacag cgacatgctg aatcagccca gcacgccgca gcctgacatt ccgttgaaaa
    19861 ctattgggcc gtaaagttcg gaccaatcac gtcaatcgca tcggtacaga tgcaatccac
    19921 gccccagcgc agcaactctg ccgcgtgctg gggtttattg acggtataaa ccagaatccg
    19981 taatccggcg tctttcaact gcatcactcg cgctttatcg agtaacttat gattgagatg
    20041 aatagagacg cagcccagcc gcgcggtcag ttcgcgccag tcgtcgcgcc actcatcaag
    20101 caataaacca cgcggcagtt ccggtgccgc ctgttgtgca gcttctaaag catcaatctc
    20161 aaacgatgac agcagcggcg gcgtcatacc ggcccacagt tggcgtgccg ccagcgccac
    20221 cattttgccc gttaatggtc cagtgccagt ggtgggtttg atttcgatat tcgccatcat
    20281 cccgtgttcg cgacagcgtt ccgccacctg cgaaagcaac ggcagcggct cacctttaaa
    20341 cgctttgctg taccaactgc ccgcatccac gcgcagtaaa tcctgccagt tcagttcacc
    20401 cgcgacgccc cagccgttgc tggtgcgttc gagattgtcg tcatggagca ggaagatctc
    20461 gccatctttc gataacttcg cgtcaaattc gatcatctta tgaccgtatt ttgccccgac
    20521 gtcgattgcc gccagggtgt tttccggggc cagcttaccg ccgccacgat gagcgacgat
    20581 gcggggataa ggccagttac tcatactcgt tgtcctgttt caccatcaaa aagatgcagc
    20641 tgattttccg ccagatgcag ccacagcgtg ctgcctgccg tcgggcgctc ctgatgcgcc
    20701 agtcgcacca ccagcttctg cttgcgagct tagcgcaata tgttccgggc gaatgccgag
    20761 ggtcatttta cgcccggcat actgacggta gccaccgttt agcggtagca cgataccgcc
    20821 gtccagttcg aaatgcgtgc cttcattatt catgcggcct gtcagcaggt tcatcgccgg
    20881 gctgccgata aaactcgcca caaacaggct ggcgggcttt tcgtagactt ccaccggcgt
    20941 gccaatctgt tcggcaacgc cgccgttcat caccattact cgctgggcga gcgtcatcgc
    21001 ctcaacctga tcgtgagtaa cgtagagtga agtcgttttc aggcgacggt gcagctgttg
    21061 cagttcaaga cgcatctgta cgcgcagctt ggcatcgagg ttagagagtg gctcatcaaa
    21121 caagaacacc gccggatcgc gcacaatcgc gcgtcccatc gccacacgct ggcgctgacc
    21181 gccggaaagc tcgcgcgggc gacgtttgag cagaccgtcc agctccagaa tgcgcgccgc
    21241 ttctttaacg cgctcggcta tttgctgctt gcccatgccg cgaattttca gcccccacgc
    21301 catgttttct tcgacgctca tatgcggata aagcgcgtaa ttctggaaca ccatcgcaat
    21361 cccgcgatct ttcggctcca tttcggtcac gcgctggtcg ttgatccaga tatcgccttc
    21421 ggtcaccctc tccagcccgg caaccatacg cagcagcgtc gatttcccgc agccagacgg
    21481 cccgaccatc acgataaatt cgccatccgc cacatcaagg gtcagcggtt taatgaccta
    21541 ggttttgcca tcccagcttt tggttactgc ctgtaatttc agtcctgcca tcttatttct
    21601 cactatcgac caggccgcgc acgaaggcac gctgcatcac taaaacaatc actactggtg
    21661 ggatcagggt taacaacatc gccgccatca ctgagttcca ttccgtggtg ccttcgccag
    21721 tggcgatcat ccctttgatc cctgccacgg tggtgccgag atccacgtcg gtaataatca
    21781 acaacggcca caaatactgg ttccagccgt aaataaaggt gatcacaaac agcgccgcca
    21841 gattagtttt ggagagcgga aaaacgatgt cgcaaaagaa gcgcattggt gacgcaccgt
    21901 cgatccgcgc cgcttccacc agttcatccg gcagcgtcat aaagaactgg cgaaacagaa
    21961 acgtggcggt cgccgaggcc atcagcggca gcgttaaacc ggcgtagctg tcgagcatct
    22021 tcaggttggc gatgacttcc accgtcggga agatacgtac ttcaaccggc agcatcaggg
    22081 tgataaaaat catccagaag aagaggttac gtagcggaaa acgaaaccag acaatggcaa
    22141 atgccgagag catcgagaca gtaattttgc cgagcgtaat gctgaacgcc atcacaaagc
    22201 tgttaagcaa catccgccag aacggcgcgc tatttgtgcc taccccgttc acccagatgt
    22261 tgtggatgtt ttccagcaga tgcgtgccgg ggatgagcgt catcggcgcg gcgtagacgg
    22321 cctgtttatc cagcgtcgcc gcgacaaacg ccacgtacag cgggaagagg atcaccgcga
    22381 tcccgaggat cagcatggta tggctgaata tcgtcagcca cggacggttc tcaatcattg
    22441 gtaacgcacc ttgctttcaa catagcggaa ctgcaccacc gtcagcacga tgacgaggaa
    22501 catcagcacc atcgactgtg cggcagacga agccagatcc agtccggtaa aaccttcgcg
    22561 gtagatctta taaatcagcg tcgtggtggc ctgaaccggc ccgccggacg tggcggcgtc
    22621 gatcaccggg aaggtgtcga agaaggcata caccaggttc actaccagca ggaagaaact
    22681 caccggggcg ataagcggca gcgcaatctt aaagaagcgg cgaatcggcc ctgcaccgtc
    22741 gatggctgcg gcttcgatca acgaacgggg aatggattgc agcgcggcat agaagaacag
    22801 aaagttgtag ctgatttgct tccacaccga ggcaaacacc accagaaaca tcgcctgacc
    22861 gctattttgc gcgtggttcc agtcgtagcc gaactcagcg agaaaatggg tgatcagccc
    22921 gcgaccgggg ttaaacagga agatccacaa tacggcggca acggcgggag ccacggcata
    22981 cggcagcaac attaaggttt ggtagaaacg actgccgcgc acgatgtact ccaccagcgc
    23041 cgcaaagaac agcgatacca gcaaaccgct gacggtgaca aaggtgctga attttatcgt
    23101 cgtccagaag gagtcgagat aatagctgtc atgaaacagc gtgacgaagt tatccagacc
    23161 gacaaactgg ctggagaaac caaacggatc gacgctttgt agcgagtacc acaacgcttc
    23221 gcccgcaggc cagataaaaa agataacggt gatgatgagc tgcggcgcga ccagcagata
    23281 aggcagccag cgcgagcgga acaccggacg ggatgatgac attacggtta attcctgaac
    23341 tgtgccggat gcgcttcgct tatccggcct acacgcgatg ccaggtcaga taagcgaaac
    23401 gcatccggca ttacactgat taagacttcg tcgatttctc aaagcggcgc agtaactggt
    23461 tcccacgctc aactgcggta tccagcgcct gctgtggcgt cttcttaccg gtccacacgc
    23521 tctcaagctc ttcatccaca atcacgcgga tctgcggcat gttgcccaga cgaagccctt
    23581 tggtgaacgg caacggcggc ttgttcagca tctgacgcgt cgcggtatcc gcacctgggt
    23641 ttttctcgta aaagccctgc tcacgggtca ggtcatacgc cgctttggta attggcagat
    23701 aaccggtttt ctgatgccac tcagcagcgt tttctggctt cgcgaggaag tcgagaaact
    23761 tcgccacgcc ggtatacgtt tctttatctt tgccctgcat cacccacagg ctggctccgc
    23821 cgataatggc gttttgtggc gcatctttcg catcggcgtc gtaaggcatc atacctacgc
    23881 cgtagttaaa tttggcgtac tcgcgaatgt tggcaagaga accggaagag gcggtggtca
    23941 tcgcgcaatc accgttatag aacttctcgg tggattcatc cttacgaccg acgtagctga
    24001 agtcgccctt cttgttcatc tcctcgagca tggcgatgtg tttcacctgc tccggcttgt
    24061 tgaactccag caccgcgtcc gtgccgtcaa agccgttgtt tttgctggca aacggcagac
    24121 cgttccaggc gctaaagttt tccagttgga tccagccctg ccagccgctg gcgtagccgc
    24181 acttaatacc ggaggctttc agttttgcgg aatagtccgc cagatcctgc caggttttcg
    24241 gcggctgttc cgggtctaac cccgctttct tgaaggcgtc tttgttgtaa tagagaacgg
    24301 aagtcgagct gttgaacggc tgggagagta agtggccggt tttgctgtcg gagtagtaac
    24361 cggaaaccgt cggcacaaac tgcgactcat cgaactgaat ccctgcctct ttaaacacgt
    24421 catacaccgg tttaatggct ttcgacgcca tcatggtggc ggtgccaact tcataaacct
    24481 gcaaaatagc cggagcgttg ccggtacgaa atacggcaat ccccgcgctt aaattctgtt
    24541 cgtagttgcc tttataggtc ggtacaattt tgtaatccgg attttcggcg ttaaaacgtt
    24601 gggccagaga atccacctct ttacccagtt ccccttccat agaatgccag aacggaatgg
    24661 tcgtcactgc ctgtgcattc cccattaacg ccagtccgag cgccagtgct gaagctgtat
    24721 aatgtaacgg tttcatcgtt tatctctctt gttgtaccga atgcgcgaat tcacgcgttt
    24781 tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt ttgttacatt
    24841 tgtaagatag taaggtgtca gaaagatgac aaggcggtga cggcgtgggt gagggaaaat
    24901 gggagatggg gcacggataa gcggaaaaat agaaggtctg aatcaaactc tacagattac
    24961 tcatcgtttc atgccggatg cggcgtaaac gccttatccg gcctacaaga tcgtgcaaat
    25021 tcaatatatt gcaactcacc cagtaggcct gataagcgca gcgcatcagg caattttgca
    25081 tttgtcacct gtctcaaagg agtcttttga ctccttatca atcaacgtgt tattacccgc
    25141 ctaaatacgc acttctcact gcttcgttcg ccagcagcgc gtcgccggta tcggagagca
    25201 ccacatggcc gttttccagc acgtagccgc gatccgccag ctttagcgcc tggttggcgt
    25261 tctgctcgac gaggaagata gtcatcccct gctcgcgcag ctgctcgatg gtgtcgaaaa
    25321 tttgctggat gataatcggc gcaagaccga gcgatggctc atcaagtaac aacaaacgcg
    25381 gattgctcat cagcgcgcga ccaatcgcca gcatctgctg ttcaccgccg gacatggtgc
    25441 ccgcccgctg aacacggcgc tcatgcagac gtggaaacag ttcatacacc cactttatgc
    25501 gctcctgaaa ctggtcacgt tcggcaaaga agccgcccat cgccaggttc tcttccaccg
    25561 tcatccgcga gaagacgcga cgcccttccg ggacaatcgc taccgcttcg cgcatgattt
    25621 tcgccgtctg ccagtcggta atgtctttat catcaaacac aattcgcccg ctggtggcgc
    25681 gcggatcgcc gcataacgtg ccgagcaagg tggttttccc cgccccgttc gcgccaatga
    25741 gagtgacaat ctcgccctga ttgatatgca ggctcacctc atgcagcgcc tggattttgc
    25801 cgtagtgggc gctgactttg tcaaaggaca acatgacttt ttccatctta tgcctcacct
    25861 aaataggcac ggatcacgtc cgggttatta cggatttgct ccggcgtacc gtttgccagt
    25921 ggcgtcccct gattgaccac ataaattcgg tctgaaatcc ccatcaccag cttcatatcg
    25981 tgttcaatca acaaaatagt ggtgttgtga tgattgcgca gttcggcaat cagctcatcc
    26041 agctctttgg tttcttttgg gttaagacct gccgccggtt cgtcgagcat taaaatctcc
    26101 ggctgcgtca ccatgcagcg ggcaatctcc agacggcgtt ggtcaccata ggccaggtta
    26161 ctcgcctgac ggttggcgtg ttccagcaaa ccaatgcgct caagccaggt ggcggcgcgg
    26221 tcgagcgctt cgctctgggc gcgacggaaa gaaggcgttt tcaacaggcc agagaacagc
    26281 ccggttttca gttgctgatg ctgcgccacc agcaggtttt caattaccgt catttcacgg
    26341 aacagacgca catgctggaa ggtgcgcacc acgcccatgc gagcaatttg ctgccccggt
    26401 aaaccttcaa gatgctgatc gcgcagtaaa atggtgccgc cggtgggttt gtagaaaccg
    26461 gtcaggcagt taaaaaccgt ggtttttccg gcaccgttag ggccgattaa cgagacgatt
    26521 tcctgcgggt acagttcaag attgacgttg ttcaccgcca gcaggccgcc gaagcgcatc
    26581 atcagaccgt taacagataa taatggctga ctcatgcctg ctctcctttc gctgcgccgt
    26641 ttttcagctt cagttgcggg cgcgtcatgg gcagcaaacc ctgcggacgc cagatcatca
    26701 tcagcaccat caaaccaccg agcattaaca tgctgtattc gttgaaatca cgcatcaatt
    26761 cgcgcgacac caccagcaga acagccgcca gaatcactgc aaattgcgag cccataccgc
    26821 cgagcaccac tatcgccagc acaaaggccg attcagcaaa ggtgaaggat tccgggctga
    26881 caaagccctg acgcgccgca aacagcgttc cggcaaaacc ggcaaatgcg gcgctgatgg
    26941 taaaagcagt cagcttgata cggcgcgggc ttaagcccag cgaacgacag gcgatctcgt
    27001 cttcacgcaa cgcttcccac gcgcggccca gcggcatccg cagcaggcgg ttaatgacaa
    27061 acaggcttaa caccaccagc agcaacgcca ccaggtagag gaagataacg cgatcggacg
    27121 gatcgtattt caggccaaag aaattactga acgtgtccca gccgccttca cgagcggtac
    27181 ggctgaactc gagtccgaag agtgtcggtt tcgggatctg actgattccg ttcgggccgc
    27241 cggtgatttc agtgttattg agcagcaata tgcgcacaat ttcgccgaaa ccaagggtaa
    27301 cgatcgccag atagtcaccg cgcagacgca gcaccggaaa accgagcagg aaacccgccg
    27361 ccgctgccat cagtccggca atcggcaaac aggtccagaa ccccagaccg taatagtgat
    27421 tgagcagcgc aaaagtgtaa gcgccgatgg cgtaaaaacc gccgtacccc agcaccagca
    27481 gaccagaaag accaacaacc acattcagcc cgagaccgag gataatgtag atcatggtca
    27541 gagtggcgat atccaccgtc ccacgtgaca ccataaacgg ccacgccacc acaagcacca
    27601 gcagcgccac gaggaacagc ttttgcttca ccgtggaccc atcaatcgcg ggcagaataa
    27661 acttcggccc ggaaacgctt ttcaaccctt tctggaaagc cggtcgcaaa agctggaaga
    27721 aaaagaccac cgccgtgccg ataaacatcc actgccaacg gatgtctgaa gccgtgtcga
    27781 ccaccagttt ggtgccatcc agctccagtt gtacgcccat aaagacgccc gccaggacaa
    27841 agaacatcgc ggcagagagc agtgccattg caatatgcat cggtttcata ctttctctac
    27901 ctccgggcga cccagaatac cggtcggcat caccagcagc accagaatca gcagggcgaa
    27961 tgagaccaca tctttatatt ccgtactcag ataggcagaa gagagcgcct ccgcaatccc
    28021 caggatcagc ccgccaatca tcgcccctgg aatgctgcca atcccaccga gcaccgcagc
    28081 ggtaaaggct ttcatcccgg ccataaagcc gatatagggg ttaatgacgc agtagaactg
    28141 accgagcagt acgccagcca ctgccgccat cgccgcgcca ataacaaagg tcaaagcaat
    28201 cacccggtcg gtgttgatgc caagcaggct cgccattttc agatcttccg cgcaggcacg
    28261 acaggcgcgc cccatacggg aatagcgaat gaaaatcgtc agcgccagca tggcgaggaa
    28321 ggtgacaatc cagatcaccg cctgcatggt ggtaatagag gcagagaagt tttcgctatg
    28381 gcccaccacc cactgaccgt taaacaggct cggcagcgcc acgtcgcgcg aaccttcggt
    28441 caggctgacg tagttttgca ggaagatgga cataccgatt gcggaaataa gcgcaatcag
    28501 gcgcttagag ttacgcaccg ggcggtaagc tacccgttcg atactccagc cgtaggcgct
    28561 ggcgatgacg attgcgccga cgaatcccgc cgccaccagc agccagccgg tatcaatgcc
    28621 catcatcatc agcgcggcga tgatcataaa tgagacgtag ctgccaatca tataaacctc
    28681 gccgtgggcg aagttgatca tgccgataat gccgtaaacc atggtgtagc cgatggctat
    28741 cagcgcgtag gtactgccca gcgtgacgcc gttaaacatc tgctgcaaga aatacaaaaa
    28801 ctgctcagac ataaggtaac ctttctaaac ccgcccgcat tttacgggcg gtgggatgat
    28861 cacttggctg ccgtggatga accgtcggcg tgccactgga agacaccaaa atcaaatccc
    28921 ttaagatcgc ctttttcatc ccagttcagc ggcccaatca cggtgtttgc accgttagct
    28981 tttaaatctt tcaccagcgc cagcggctca tcgctgccgg tacgctcaag ggcagttgcc
    29041 agagattgca ctgccgcgta agtgatccag acataaggcc cggacggatc tttcttgtct
    29101 gctttcagcg catcaacgat gccctggttt gccggatcct ggtcatagcg ttttggcata
    29161 gtgaccaaca tgccttcggc ggcatcaccg gcaatgttcg acagcgatgc gttaccgaca
    29221 ccttccggcc ccataaactg ggttttcagg ccaacggaac gggcttggcg cagcatctgc
    29281 cccatttccg ggtagtaacc gccgtagtaa acgaagtcga tattttcttt tttcaggcgg
    29341 gcgatcagcg cggagaaatc tttctccccg gcggtaatac cgtcgaagaa gacgacgttg
    29401 gcgttagccg ctttcagccc gtcctgcacc gaacgcgcca gcccttcgcc atactgttgt
    29461 ttgtcgtgaa tgatggcgat gcgctggggc ttcaccgtct caagaatgta ttttgccgcc
    29521 gtaggcccct gggaagagtc cagcccggca gtacgcataa tgtgttgata accgcgttgg
    29581 gtcagctccg ggttggtcgc tcccggcgag atcatcagaa taccttcgtc ttcgtagata
    29641 tccgacgctg gctgggtaga ggaagaacac agatgaccaa taacgtattt aatgccgtcg
    29701 ttaacgattt tgttggcgac cgcaacggct tgtttcgggt cgcaggcgtc gtcatattcc
    29761 acgccaacca gcttgtcgcc cttaattccc cctttgacat taatgtcttt aatcgcctga
    29821 cgcgcgccgt taaattccat atcgccccac tgggcaatcg ggccggacat cgcgccgaca
    29881 acggcgactt taatatcgtc agccatagcg gtgtgtgaaa ttgccagtgc aatcatccct
    29941 gcgatgatag ttttcgcatt ccgtttcata gtcaaaaatc cccattcgtg atgttgtgtt
    30001 gctttgtttt tatgtgttaa caaatcagac tgttcttttt ttatactgca ctgtttttgc
    30061 ctgtctgatt aaggggttag cgcagtattt tgtgatgata gcgattaaaa tccctatttt
    30121 tcagtcgatt aagaacagat aatattctga atttattgat agataaacag aaaaaagtgc
    30181 ctttgtcagc ataaaataac ggcacaaagg gcggaataat tcactatcat tcaggggatt
    30241 atgctggaca tttttcattc tctaatgttt taactttgta attattgctg ttaaaaaatt
    30301 aatcacctgc caaaagaaat aaaaaagaga aagcctccga ttaaattatt tcgctacact
    30361 ggttccactt ttgtgattta cacgggttac ccatgaagct gaccatcatt cgattagaaa
    30421 actttagcga ccaggaccgg attgacctgc aaaagatctg gccggagtat tccccttcct
    30481 ctttacaggt tgacgataac caccgtatct acgccgcgcg ttttaacgag cgtctgcttg
    30541 cggccgtgcg ggtaacctta agcggcaccg agggagcact ggattctcta cgcgtgcggg
    30601 aagtcacccg ccgtcgcggt gtggggcaat atctgctgga agaggttttg cgtaacaatc
    30661 ctggcgtttc atgctggtgg atggcggatg caggcgtgga agatcgcggt gtgatgacgg
    30721 cgtttatgca ggcactggga tttacggcac agcagggcgg ctgggagaag cgttaatcgt
    30781 caagttgatt tcaaaagtga tgaccaacga gaaattgcct gatgcgctac gcttatcagg
    30841 cctataatgt gtgctgcaat ttactgattt ctttggttct tgtaggccgg ataaggcgtt
    30901 tacgccgcat ccggcatgaa gcaacgtatt cgatattagc aatttggcgg caacccaaag
    30961 ttgccgatta acaattactt cgcatcggtc gccgtgccgt tggcgtgcca gtcaaatacg
    31021 ccgaactcaa agccctttag atcgcctttc tcatcccagg tcagtggccc catcacggta
    31081 tccacggagt tcgcttttag gtatttggcg atttcagccg gatcgtcaga ctgattcagg
    31141 cccgcctgca aagattgcag cgcggcgtag gtggtccaga cgaatgcgcc gcttgggtcc
    31201 tgttttttcg ctttgatcgc atcaacaatg ggtttgttcg ccggaacctg atcgtagttc
    31261 ttcggcttgg tcaccagcag cccttccgct gattcgcccg caatgttaga cagcgaaacg
    31321 ttagccacac cttccggccc cataaactga gttttcagcc ctgccgcgcg tgcctgacgc
    31381 aggatttgcc ccatttccgg gtgataaccg ccgtagtaaa cgaagtcgat attctctttt
    31441 ttcagacgcg ccaccagcgt tgagaaatct ttttccccgg cggtgatacc atcaaagaac
    31501 accacgttag cattgccttt cttcaggccg tcctgcaccg ctcgtgccag accttcgccg
    31561 tattgctgtt tgtcgtgaac gatagcaata cgctgcggtt tcactttctc aagaatatat
    31621 ttcgccgccg tcggcccctg gtcggagtcc aggccggtgg tgcgcaggat cagctgatag
    31681 ccacgggcgg tcagctccgg cgcggttgcc gctggggtga tcattaaaat gccttcgtct
    31741 tcgtagatgt cagacgcagg ctgcgttgat gaagaacaga ggtgaccaat cacatattta
    31801 atgccgtcgt taacgacttt gttcgccacc gcaaccgcct gtttcgggtc acaggcatcg
    31861 tcatatttta cgatttgcag tttgttgcct ttaatgccgc ctttagcgtt gatatccgca
    31921 accgcctgct ctgcgccggt aaactcctgg tcaccgtact gcgcaaccgg accggacatt
    31981 gcacccacga ccgcgacttt aatatcttct gccagagcca tattgctgaa tgccagcgcg
    32041 atacatcctg ccagtaacgc tttacccttt atgttcatcc tgagaatccc cattcttctg
    32101 gttattacgt gtgttgtgat gttgttgttc agcactttat ttcgttttat gcatgactac
    32161 ccgtgcttta gcagcatact ctgctaaaac atacccgatt tttatgatat tggaatagct
    32221 attttgacag tttattaaca atctgcgtgg ggattggcgt tttgccggag ggggaattga
    32281 ttattgtaga ggcccaaaaa acaaaaaccc cggactctca tccagggttc tctgcttaat
    32341 agcggaaatt acgcttcaat agcggcgcga agttttttca tcgcgttctt ttccagttgg
    32401 cgcacacgtt cagcggaaac accgtaacgg tcagccagtt cctgcaacgt ggacttgttg
    32461 tcttcgtcca gccagcgcgc acggatgatg tcctggctgc gttcgtccag accatgcatc
    32521 gcgtcggtca gacggtttgc cgcctgctct tcccagttat catcttcgat gccgtcggca
    32581 aagttagatg atttatcctg cagatagagc accggtgcca tcggctggct gtcggaatcg
    32641 tcgtcggaag acaggtcaaa ggtcatgtcc tgtgccgcca tacgtgattc catctcacgt
    32701 acgtctttgc tggttacgcc cagttcacgg gccaccattt cgacttcatc ctggttaaac
    32761 cagcccagac gctgcttggt tttacgcagg ttgaagaaca gtttgcgctg cgctttggtg
    32821 gtcgcaactt tgacgatacg ccagttacgc agaacgtatt cgtggatctc tgctttgatc
    32881 cagtggacgg cgaaggagac caggcgcaca cccacttccg ggttaaaacg gcgcactgct
    32941 ttcatcaggc cgatgttacc ttcctgaatc aaatccgcct gtggcaggcc atagcccgca
    33001 taattacgag caatatgaac aacaaaccgc aggtgagaca ggatcagcgt tttagctgct
    33061 tccagatcgc catggtaatg cagcttttca gccagcgccc gctcctcgtc agccgacaac
    33121 atcggccacg cgttagctgc ccggatgtag gaatccaggt tgccaactgg ggctaaagct
    33181 aaactttgca ttttgtcagt cattcaaatc ctctcaatcg atatcttctg gcgcttcagt
    33241 ggtagcaaca actgtgccaa agcttaagag caacgaggtt atcattcact gttttatcag
    33301 accgtgattt tatccacaag ttcaatgcaa gcttgtgaat aaattacgca caaaatgtga
    33361 catagagatg aaataccggg aagagacaac ggggtctctt tccctgctac ggaacccatt
    33421 gcagggaaag agtataacac gcttttatta ttcaggcgta aagtggcgta aatgttgtac
    33481 cgtggcaagc cacgctgcca cccagccaat catcgagcat accagcagca atagcaggca
    33541 ttcatcgaac gataaaccat tgatatcaaa cttcgttccg aaaacctgtg ccacttccgc
    33601 caccgctgac gacaaacgca gtaccagaat ttctgacaaa attaatgaca acaatgcgcc
    33661 agaaaatccc agcagcgcac cgccgtacag gaacgggcga aggatgaaac catccgtcgc
    33721 accaatcagt ttctgcacgt tgatggagtc acggcgagca aagatgctca gacgtacgct
    33781 gttgccgatg acgaggaaca ctgccgccac catcaacacg ccgatcatcg ccgaaacgcg
    33841 cccaaccagc ccggtcaacg ccgccagacg ggcaaaccag ctgtcatcca tccgcacttc
    33901 gtcaataccg ttaatctgcg tgatacgatc acgcagcgta ttgagtgatt ccgtcccctg
    33961 aaaatcgagt ttcgggatca ccaccgccac tgccggaagc gggttttctt ccagcatatc
    34021 cagcgcacca ccaaaaccag accagttacg gaactcgccc agtgcgtctt cacgagaaag
    34081 atagttcact ttctccacgc cttgctcggc ctgcaactgt gccaccacgc ccgcagcggc
    34141 gtcatcgtcc agcgtttttt gcaagtaaac agtgatttgc ggtgacggat aatactgcgt
    34201 cgccgcctga ttaacgtttt tgtacaccat ataacagacg ctgggcagcg tcagggagat
    34261 ggcgataacc atcaccgtta aaaacgtggc gaacggtttg cttttcagat cctgcaatgc
    34321 gccgtggaag gcatagcgca cctgttcgtt gaaaacgttg gttttgcgat ttaccggttt
    34381 tggcgaggat ttcgcgcgtt tcggtgcgtt acgaccgccg tcgcctgagc cgccgaccga
    34441 tttacggaag cgatccagac gcccaccaaa ctgccgaata tgattgattg catcgcgctt
    34501 attcatggcc cacgcctcca tgcaagtgac catcgctcag ggtgagcatg cgataggaac
    34561 gccgtgaaat cagattaatg tcgtgcgttg ccatcaatac ggttacccca acgcggttaa
    34621 actcttcaaa cagacgtaga atcccttccg acaacgcgtc gtccaggtta ccggtcggtt
    34681 cgtccgccag cagtaccgcg ggcttattca ccaccgcgcg ggcaatgcca acacgctgtt
    34741 gttcaccgcc ggaaagctga atagggaagt tcttcgcttt gtccagtagc ccgactttat
    34801 ccagtgccgc cgacacccgg cgacgaatat cgtcaccgct ggcaccagcg ataatcagcg
    34861 ggatcgccac gttatcgtag acagtacggt ccatcagtag atggtgatcc tggaaaatca
    34921 tgccaatctg gcggcgcaga aacggaactt cacggttttt cagacgcgtg atgtcatggc
    34981 cgctaaacca gattttcccg gcgctgggcc gctcaatccc acagatcagc ttcaggaggg
    35041 tacttttccc tgcgccggaa tgaccggtca gaaacgccat ctcacccggc tgcatatgga
    35101 acgtaacgcc ctgcagcgcc tgtctcccac cgagataagc cttgctgaca tgttcaaagc
    35161 gaatcattgt taatcctctc gggcaaaaag tgcctctata aagtcgtccg ccttaaacgg
    35221 acgcaaatcc tcaatacgtt cgccgacacc aatgtagcgg atagggatac caaactggtc
    35281 agccaccgag aaaattaccc cgcctttcgc cgtgccgtcc agtttcgtta gcgtgatgcc
    35341 ggttaagcca acagcttcgt ggaacagttt ggcctggctt accgcgttct gcccggtgct
    35401 ggcatcaata gtcagcataa cttcatgcgg cgcttcaacg tcgagtttct tcatcacgcg
    35461 gacgattttc ttcaactctt ccatcagatg cgatttgttc tgcagacgtc cggcggtatc
    35521 ggcaatcagg acgtcgatat tacgcgcttt agctgcctga atggcgtcga agataacaga
    35581 ggcggaatcc gcaccggtat gctgggcaat caccggaata ttgttgcgct gaccccagac
    35641 ctgaagctgt tcaaccgcag ctgcacggaa agtatcgccc gccgccagca tcaccgattt
    35701 accctgctgc tcaaactgac gcgccagctt accaatcgtc gtggttttac ccacaccgtt
    35761 gacgcccacc atcaggatca cgaacggcgt tttatcttca acattcagcg gctcatcgac
    35821 tttcgccaga atctcgccca tctcttcttt cagcaggcca tagagcgcct cggcatcacg
    35881 aagctgcttg cgggatgcgc cttccgtcag attggtgata attttacgtg tggtttccac
    35941 gcccacatcg gcgatcaaca gctgttcttc cagctcttca aacagatcat cgtcgatttt
    36001 tttaccgcgg aacaggctga taaatccgga accgagattt tctttggttt ttaacaggct
    36061 gcgtttcagg cgcgcgaaga agccttcttt ggtcggtttt tcttgctcct gggcaatttc
    36121 tgcaaccggc tgctcttctt ctgccggagg aactaccatc accgcctctt ctgccgcttc
    36181 ggcagccagc gcctgtgctt ccagctcttc gtcggtgatt tcttctttag ccgcttcttc
    36241 ttcttctgcc gcttcgacaa tctctacggt ttccgcttca gcctgccact cttctgacga
    36301 aacctcttcg gcgttgacgt cttccggcaa cggcagctct tcacgttcga tagccactgg
    36361 ctccggcgtt tcttctacga ccggttccgg ctgtgcaaca acttccgctt caggctgcgc
    36421 tttttcactt tcaacaacct gttcagtgac ttccacaacg tcggcagcaa aagtttccgc
    36481 ctcggcttca gtatgcgcct gcggctgctc ttcaacggct tgttcagaag ccttcgcagg
    36541 ctcttgcgcc tgaacgattt cttctacaac cggttgttca ttctgaactt ctgtctcttt
    36601 ttccggggtc tgctcttttt gaccaaagcc cagccaggaa aaaaagccac gtcttttttc
    36661 tttcgccatt tgcgactaca ctcctcgctg ttgcttcatg gcacagcgtt aacgctatgt
    36721 acatagcagc taaaaaaatg atgaaatagt ctatcactta acttaattca catcaccgcc
    36781 tgcaagtatg tgttatctgg cggattgagc aatttatcat gaaaaaaccg aatcattccg
    36841 gcagcggcca aatccgcatt attggcgggc agtggcgagg ccgtaaactc ccggttcctg
    36901 atagcccagg tctgcgcccc accaccgacc gcgtacgcga aacgttgttt aactggctgg
    36961 ctccggtcat tgttgacgcc caatgtctgg attgcttcgc cgggagcggc gcgctggggc
    37021 tggaagcgtt atcgcgctac gctgcggggg caacgttgat tgagatggat cgcgtggttt
    37081 ctcagcagtt aattaagaat ctggcgacac taaaagcagg caatgcacgc gtggtgaaca
    37141 gcaacgcaat gtcattcctg gcgcaaaaag gcacaccgca taatatcgtg tttgtcgatc
    37201 caccgttccg ccgtggcttg ttagaagaga cgataaattt actggaagat aacggctggc
    37261 tggctgacga agccctgatt tatgtcgaaa gcgaagtcga aaacggtctg cccactgttc
    37321 cggcaaactg gtcattacat cgggaaaaag tggcgggtca ggtggcttat cggctgtatc
    37381 aacacgaagc acaaggagaa agtgatgctg attaatattg gtcgtttgtt aatgctctgc
    37441 gtttggggat ttttaatcct caatctggtg catcccttcc cgcgcccgct aaatattttc
    37501 gttaacgtgg cgctgatttt taccgtgctg atgcatggta tgcagctggc gctattgaaa
    37561 tccactttac cgaaagatgg cccgcagatg accaccgccg aaaaggtgcg gattttcctt
    37621 ttcggcgtgt ttgaactgct ggtctggcag aagaaattta aagttaaaaa ataaccttat
    37681 tgttcgccaa caaagctaac aaagcgcgtt cctttatagc tcagcgtacc tttatcgccc
    37741 actgtcaggg cgtggtactg ctgggcgtcg aggcgaaacg tctgctccat tcctccgctt
    37801 tgcggtttga agcttgcctc atagcgcata ctggtgcctg ccggagtcac ttcctgctgg
    37861 cgcgaacggc gatcgttaat cggtttttcc cgcttgttgc tcaccaccac cagcttttgc
    37921 tggagcggag ccatatcatt atcagctttt tcccgtcgct gttgcataaa acgaaacgat
    37981 gcggcgacga caattaagcc aatgataaca ataaagaaaa gaggtggttt actcatcttt
    38041 atccctcatc ggaaaatgcg gaattaagca taccctgcca gttatggtgt tgtcatccgt
    38101 ccaccctcgc cactaaactg gaagcaagac cgtaggcatt ctgcttacga aaaaataacg
    38161 aattcaagga actaagatgc tttggtcgtt tatcgctgtc tgtctttccg catggctatc
    38221 tgtggatgca tcgtatcgtg ggccaacctg gcaacgctgg gtgtttaaac cgttaaccct
    38281 tcttctcctg ctgttactgg cctggcaagc accgatgttc gacgccatta gctatctggt
    38341 gctggcggga ctgtgcgcct cactgctggg cgatgcgcta accctgttgc cacgtcaacg
    38401 tctgatgtac gccatcggcg cgtttttcct ctcgcacctg ctgtacacca tctatttcgc
    38461 cagtcagatg acgctctctt tcttctggcc tctgccactg gtgttgctgg ttctgggtgc
    38521 gctgctactg gcgattatct ggacgcggct ggaagagtac cgttggccta tctgcacgtt
    38581 tatcggcatg acgctggtga tggtgtggct ggcaggtgaa ctgtggttct tccgtccgac
    38641 cgctccggcg ctctctgcgt ttgttggcgc ttcgttgctg tttatcagta actttgtctg
    38701 gctggggagc cactatcgcc gacgcttccg tgcggataac gcgattgctg cggcctgcta
    38761 ctttgccggt cacttcctca tcgtccgctc actgtatctc tgataaaact tgactctgga
    38821 gtcgactcca gagtgtatcc ttcggttaat gagaaaaaac ttaaccggag gatgccatgt
    38881 cgacacctga caatcacggc aagaaagccc ctcaatttgc tgcgttcaaa ccgctaacca
    38941 cggtacagaa cgccaacgac tgttgctgcg acggcgcatg ctccagctcg ccaacgctct
    39001 ctgaaaacgt ctccggcacc cgctatagct ggaaagtcag cggcatggac tgcgccgcct
    39061 gtgcgcgcaa ggtagaaaat gccgtgcgcc agcttgcagg cgtgaatcag gtgcaggtgt
    39121 tgttcgccac cgaaaaactg gtggttgatg ccgacaatga catccgtgcc caggttgaat
    39181 ctgcggtgca aaaagcgggc tattccctgc gcgatgagca ggccgccgac gagccacaag
    39241 catcgcgcct gaaagagaat ctgccgctga ttacgcttat cgtgatgatg gcaatcagct
    39301 ggggtctgga gcagttcaat catccgttcg ggcaactggc gtttatcgcg accacgctag
    39361 ttgggctgta cccgattgct cgtcaggcat tacggctgat caaatccggc agctacttcg
    39421 ccattgaaac cttaatgagc gtagccgcta ttggtgcact gtttattggc gcaacggctg
    39481 aagctgcgat ggtgttgctg ctgtttttga ttggtgaacg actggaaggc tgggccgcca
    39541 gccgcgcgcg tcagggcgtt agcgcgttaa tggcgctgaa accagaaacc gccacgcgcc
    39601 tgcgtaacgg tgagcgggaa gaggtggcga ttaacagcct gcggcctggc gatgtgattg
    39661 aagtcgccgc aggtgggcgt ttgcctgccg acggtaaact gctctcaccg tttgccagtt
    39721 ttgatgaaag cgccctaacc ggagaatcca ttccggtgga gcgcgcgacg ggcgataaag
    39781 ttcctgcagg agccaccagc gtagaccgtc tggtaacgct ggaagtgctg tcagaaccgg
    39841 gtgccagcgc cattgaccgg attctgaaac tgatcgaaga agccgaagag cgccgcgccc
    39901 ccattgagcg gtttatcgac cgtttcagcc gtatttatac gccagcgatt atggccgtcg
    39961 ccctgctggt aacgctagtg ccgccgctgc tgtttgccgc cagctggcag gagtggattt
    40021 ataaaggtct gacgctgctg ctgattggtt gcccgtgtgc gttagttatc tcaacgcctg
    40081 cggcgataac ctccgggctg gcagcagcag cgcgtcgtgg ggcgttaatt aaaggcggtg
    40141 cggcgctgga acaactaggt cgtgttaccc aagtggcgtt tgataaaacc ggtacgctga
    40201 ccgtcggtaa accgcgcgtt accgcgattc atccggcaac gggtattagt gaatctgaac
    40261 tgctgacact ggcggcggcg gtcgagcaag gcgcgacgca tccactggcg caagccatcg
    40321 tacgcgaagc acaggttgct gaactcgcca ttcccaccgc cgaatcacag cgggcactgg
    40381 tcgggtctgg cattgaagcg caggttaatg gtgagcgcgt gttgatatgc gctgccggga
    40441 aacatcccgc tgatgcattt gctggtttga ttaatgaact ggaaagcgcc gggcaaacgg
    40501 ttgtgctggt agtacgtaat gatgacgtgc tgggtatcat tgcattgcag gataccctgc
    40561 gcgccgacgc tacaactgcc atcagtgaac tgaacgcgct gggggttaaa ggagtgatcc
    40621 tcaccggcga taatccacgc gcagcggcgg caattgccgg ggagctgggg ctggagttta
    40681 aagcgggcct attgccggaa gataaagtca aagcggtgac caagctgaat caacatgcgc
    40741 ccctggcgat ggtcggtgat ggtattaacg acgcgccagc gatgaaagct gccgccatcg
    40801 ggattgctat gggtagcggc acagacgtgg cgctggaaac cgccgacgca gcattaaccc
    40861 ataaccacct gcgcggcctg gtgcaaatga ttgaactggc acgcgccact cacgccaata
    40921 tccgccagaa catcaccatt gcgctgggac tgaaagggat cttcctcgtc accacgcttt
    40981 tagggatgac cgggttgtgg ctggcagtgc tggcagatac gggtgcgacg gtgctggtga
    41041 cggcgaatgc gttaaggttg ttgcgcagga gataaggcaa accgatcgca acattgagcg
    41101 cgatcggtcc cctcgcccct ctggggagag ggttaggggg tgaggaaaag gcggcatcga
    41161 agccaatcag cccctatcaa ccgcctttac gaatcaaata acgataaggc agtccatccg
    41221 tctctttcgc aaccagttcg tgttccataa aggtacaaaa cccaggaata tcgcgggtag
    41281 tggccggatc gtcggcgata atcagcaacg tttcgccagg ctgcatattg cgcacggttt
    41341 tgcgcaccat catcaccggt tccgggcagc gcaggccaag cgcgtcgagt gtgtggtcag
    41401 ggctggaaaa gagatcggtc attttcttct catcacttaa aaaaacggcg ctagtttacg
    41461 ccctgtgagt ccgtaagcaa accaggttaa cgattgcgtg aaaattaacc attgcattgt
    41521 caacgtaaag cagtatcatg cggcggctcg aaaaaagggt aagcacgtta ttatgttaag
    41581 gtaacagacg tgtcgtacgt attgggttcc ctcaccccaa tggttaatca aaaaggtaca
    41641 atatgaacgt tttctcgcaa actcaacgct ataaggcgtt gttctggtta tcgttatttc
    41701 atcttctggt gatcacctcc agtaactatc tggttcagct tcccgtctcc atttttggtt
    41761 tccataccac ctggggcgcg tttagctttc cgtttatttt tcttgctacc gacctgaccg
    41821 tgcgtatttt tggcgcaccg ctggcccgac gcattatctt cgcggtaatg atccctgcgt
    41881 tattaatctc ctacgtcatc tcgtcgctat tctatatggg ttcctggcag ggattcggcg
    41941 cactcgccca cttcaacctg tttgtcgccc gtatcgccac cgccagtttc atggcctacg
    42001 cgctggggca aatcctcgac gtgcacgttt ttaaccgcct gcgtcagagt cgccgctggt
    42061 ggctggcacc gacagcgtcc acactgttcg gtaacgtcag cgacacgctg gcctttttct
    42121 tcattgcctt ctggcgtagc ccggatgcct ttatggctga acactggatg gaaatcgcgc
    42181 tggtcgatta ctgtttcaaa gtgttaatca gtatcgtttt cttcctgcca atgtatggcg
    42241 tattactcaa tatgctgttg aaaagactgg cagataaatc cgaaatcaac gctttgcagg
    42301 cgagttaaag gttcgttatc agagttgtga taagatggat gaatgagccg ttatggccgt
    42361 ttatcgaaag gaagaagtca atgcgcaatc tggttaaata tgtcggaatt ggcctgctgg
    42421 ttatggggct tgcggcctgt gatgataaag acactaacgc tacggcgcag ggttcggttg
    42481 cggaaagtaa cgctaccggg aatcccgtca acctgcttga tggcaagtta agtttctcgc
    42541 tgccagcgga tatgaccgac cagagcggta agctgggaac gcaggccaat aacatgcatg
    42601 tctggtccga cgccaccggg cagaaagcag tcatcgtcat catgggcgat gatccgaaag
    42661 aagatctggc ggtgttggcg aagcgtctgg aagatcagca acgtagccgc gatccgcagc
    42721 tgcaagtggt aaccaataaa gccattgagc tgaaaggtca caaaatgcag cagttagaca
    42781 gtattatctc cgcgaaaggc cagacggcgt actcttccgt tattctgggt aacgtgggta
    42841 atcaactgct gaccatgcaa attacgctgc cagctgacga tcagcaaaaa gcgcaaacca
    42901 ccgcagaaaa catcattaat acgctggtta ttcagtaagt tttaagatga tgaggcggcc
    42961 tcagggacgt gttccggagg ccgttttttt aatcgccacg tcagtaataa cgcgattgcg
    43021 accagtcccg ccgccgccag ataaatcacc ggtacgcccg cccagctcat caccagccca
    43081 gccagtggtc cagtcacgcc aagcgataaa tccataaata cggtgtaagt tgccagcgcc
    43141 gccccctgat tttgctgcgg aaccgctttt accgccacta cacccaatgc cgggaacacc
    43201 agcgaaaacc cggcccccgc cagtaagacg ccgattttcg ccatccacgg catagtcgcc
    43261 acgccaacca gtagcaggcc gattatctca acgctaaagc aaatcatcgc tacgtttaag
    43321 ccaccgatac ggttaatgcc gttagggaat aacaaacgcg taccgacaaa cgcacagcta
    43381 aacagcgtca gcgcgaaagc cgcaccgtcc caacctttag cgtcataaaa cagcgtgata
    43441 aaggtggcga tgacgccaaa tccggcggaa gccagtgcca gcgccatacc gtacagccag
    43501 acgcgcccaa gcaccgcgcg aaacggcagc ggtttgcctt tactggcttt taccgtcgga
    43561 cgcgggatcg ccaacaaaat ggccaccagc gccacgccca taatgattaa cgctaacgcc
    43621 tgcaagccgc cccagtgata aaacacgacg cctaacggcg cacccatcgc catcgccccg
    43681 taagtgacaa tgccgttcca cgaaatcacc cgcccgatat gcagcgagcc aaccactcca
    43741 acgccccata gggtcgatcc cgttccggca aaactttgcc caatcccaag gatgacgcgc
    43801 cccaggcaaa gtaataacag gctgatgaca ggcagactgg cggttaatcc tgccgtcaga
    43861 taccccagac cgctcaaaaa gcagccgcat aaaccgaaga cgacaatctt tttgggtccc
    43921 agcaaatcgg cgtaacgtcc ggcatgaggg cggctcagca aggtggcgaa atattgcagg
    43981 ctgataacca atcctgccca gaaggcgcta aagcccatca catcatggac atagcccggt
    44041 aatacagcga gcggcaaccc gatggtgagg tagctggcga agttaaacat gacaatggag
    44101 acaatgcgca aattcaggcg caatccgttt agcgcgggtt cggctacggg ttcgggcatg
    44161 aggatcacca catttttaca acagtgtttc atttttacca tgtgctgacg tgaaaatcag
    44221 cagtaagaat cagaatattg ctggcgtgac tcccgcgcta cacttaacac aaaaagtcac
    44281 aaggaagccc caatggaaac ccctcaaccc gataaaacgg gcatgcacat tctgctcaag
    44341 ttggcctcgc tggtagtgat cctcgcgggc attcacgcag cggcagatat cattgtgcag
    44401 ctgttactgg cgctgttttt tgccatcgtc ctcaacccgc tcgttacctg gtttattcgt
    44461 cggggagtac aacgccccgt tgccattacg attgtagtgg tggtgatgct gatcgcacta
    44521 accgcgctgg tcggcgtact ggcggcatcg tttaacgaat ttatctctat gctgccgaag
    44581 tttaataagg agctgacgcg caaacttttt aaattgcagg agatgttgcc ttttcttaat
    44641 ttgcatatgt cgccggagcg aatgctgcag cggatggact cggaaaaagt ggttaccttc
    44701 accacagcgc taatgaccgg gctttccggg gcaatggcga gcgtgctttt gctggtgatg
    44761 accgtagttt ttatgctgtt tgaagtgcgc cacgtccctt acaaaatgcg ttttgcgctg
    44821 aataacccac agattcacat cgcaggatta caccgcgcac ttaaaggcgt ttcgcattat
    44881 ctggcgttaa agacgctact cagtttatgg acaggtgtca tcgtctggct ggggctggcg
    44941 ctaatgggcg tacagtttgc gctgatgtgg gcagtactgg cgtttttgct caactacgtg
    45001 cccaatatcg gcgcggtaat ttccgccgta ccgccaatga ttcaggtgct gctgtttaat
    45061 ggtgtttacg aatgtattct ggtcggcgca ttgtttttag tggtccatat ggtcatcggc
    45121 aatattttag aaccacggat gatgggccat cgcctgggga tgtccaccat ggtggtattt
    45181 ctttcattgt taatctgggg atggctgctc ggcccggtag ggatgctact ttcggtgcca
    45241 ttaaccagcg tgtgtaaaat ctggatggaa acgacgaaag gtggcagcaa actggcgatt
    45301 ttactggggc cgggcagacc gaaaagtcgg ttaccgggat gaggcgacaa gtgatacgat
    45361 acgcactttc attttccatt aaacgttggc cctgatatgt atcggatagt tctggggaaa
    45421 gtttcgacct taagcgcagc tccactgcca ccgggtttac gcgagcaagc accgcaaggt
    45481 ccacgacgcg aacgctggct ggcggggcgt acattgcttt cgcacacgct ttccccgcta
    45541 ccggagatca tctatggcga acaaggcaaa cctgcattcg cgccggaaac gccgctatgg
    45601 ttcaacttaa gccatagcgg tgacgatatc gctctgctgt tgagcgatga aggcgaagtc
    45661 ggctgcgata tcgaagtgat tcgcccgcgc gtcaactggc gctggctggc gaacgccgtg
    45721 ttcagcctcg gggaacacgc tgaaatggac gccgtgcatc ctgatcagca actggaaatg
    45781 ttctggcgca tctggacgcg caaagaagcc atcgttaaac agcgtggcgg cagcgcctgg
    45841 caaatcgtca gcgtagatag cacctatcac tcctcgctgt cagtcagcca ttgtcagcta
    45901 gaaaatttaa gccttgcgat ctgcaccccc acccccttta cgctcaccgc cgacagtgtg
    45961 caatggatcg attcagttaa ctgatccgcc cacccgactg cccatctatt gatccagaac
    46021 aggtaatcag tatgacgaat acttaaaatc gtcatactta tttccgccat ctattttaat
    46081 ccattggggt taccatgctc tccacactcc gccgcactct atttgcgctg ctggcttgtg
    46141 cgtcttttat cgtccatgcc gctgcaccag atgaaatcac caccgcctgg ccggtgaatg
    46201 tcgggccact aaacccgcac ctttacacgc ctaaccagat gttcgcccag agcatggttt
    46261 atgaaccatt ggtgaaatat caggcagacg gttcggtgat cccgtggctg gcaaaaagct
    46321 ggactcattc agaagatggt aaaacctgga ccttcaccct gcgtgatgac gtgaaattct
    46381 ccaacggtga accgttcgat gccgaggcgg cggcagaaaa cttccgcgca gtgctcgata
    46441 accgtcaacg tcacgcctgg ctggagctgg caaaccagat tgttgatgtt aaagcactca
    46501 ataaaacaga gctgcaaatt accctgaaaa gcgcctacta tcctttcctg caagaactgg
    46561 ccctgccccg tcctttccgt tttatcgctc cctcgcagtt taaaaaccat gaaaccatga
    46621 acggaattaa agcgccgatt ggcaccggac cgtggatttt gcaggaatcg aaactaaatc
    46681 agtacgatgt cttcgtccgt aacgaaaact actggggcga aaagccagcg attaaaaaga
    46741 tcacctttaa cgtcatcccg gacccgacta cccgcgcggt ggcgtttgaa actggcgata
    46801 tcgacctgct gtacggaaac gaagggttat taccgctcga taccttcgcc cgctttagcc
    46861 agaatccggc ttaccacacc caactgtcac agccgatcga aaccgtgatg ctggcgctca
    46921 ataccgccaa agcccccacc aacgagctgg cagtacgtga agctcttaat tacgcggtaa
    46981 acaaaaaatc gctgattgat aacgcgttat atggcaccca gcaggtcgcc gacaccctgt
    47041 ttgccccttc tgtgccttac gctaatctcg gcctgaaacc gcgccagtac gatccgcaga
    47101 aagcgaaaga gttgctggaa aaagccggtt ggacgctgcc tgcgggcaaa gacatccgcg
    47161 agaaaaatgg tcagccgctg cgcattgaac tgtcgttcat cggcaccgat gcgttaagca
    47221 aatcgatggc ggaaatcatt caggctgata tgcgccagat tggcgcagat gtctcgctga
    47281 ttggcgaaga agagagcagt atctatgctc gtcagcgcga cggtcgtttt ggcatgattt
    47341 tccaccgcac ctggggcgcg ccatacgatc cacacgcctt cctgagttca atgcgcgtac
    47401 cgtcgcacgc tgacttccag gcacagcaag gattagccga caagccgctg attgataaag
    47461 agatcggcga agtgctggcg acccatgacg aaacgcaacg tcaggcgctg tatcgcgaca
    47521 ttctgacccg tctgcatgac gaggcggttt atctgcctat cagctacatc tcgatgatgg
    47581 tggtatcaaa accggagctg ggtaatatcc cctacgcgcc gatcgccacc gaaattccgt
    47641 tcgaacagat taaaccggtg aaaccttaat gttgcgttac gtattacgcc gctttctgct
    47701 gctgatcccg atggtgctcg ccgcctcggt gatcattttt ctgatgctgc gcctcggtac
    47761 cggcgacccg gcgctcgatt atctgcgttt atctaacctg ccgccgacgc cggagatgct
    47821 ggcctctacc cgcaccatgc tcgggctgga tcagccgctg tacgtccagt acggtacctg
    47881 gttgtggaag gcgctgcatc ttgactttgg tatctcattc gccagccaac gcccggtact
    47941 ggacgatatg ctgaacttcc tgcccgccac gctggaactt gcaggtgcgg cgctggtatt
    48001 aattctgctc acttccgtac cgctcggtat ctgggcggcg cgccaccgcg atcgtttgcc
    48061 ggatttcgcc gtgcgcttta tcgcgtttct tggcgtgtcg atgcccaact tctggctggc
    48121 atttttgcta gtgatggcgt tttcggtgta tctgcaatgg ctacccgcga tgggctacgg
    48181 cagctggcag cacatcattt tacctgcggt ttccatcgcc tttatgtcgc tggcgattaa
    48241 cgcgcgactg ctgcgcgcca gtatgctgga cgtcgccggt cagcgtcacg tcacctgggc
    48301 gcggctacgc ggcctgaatg acaaacagac cgaacgccgc catattctgc gcaatgcctc
    48361 gctgccgatg atcaccgccg tggggatgca cattggcgaa ttgattggcg ggacgatgat
    48421 tatcgaaaac atctttgcct ggcctggcgt cgggcgctat gcggtgtcgg cgatttttaa
    48481 ccgtgactat ccggtgattc agtgctttac gctgatgatg gtggtggttt ttgtggtctg
    48541 taatttgatt gtcgatttgc tcaacgccgc gctggacccg cgcattcgtc gtcatgaagg
    48601 agcgcacgcg tgaacttttt cctctcttcc cgctggtcgg tacgcctggc gctgatcatt
    48661 atcgccctgc tggcgctgat tgcgctcacc agccagtggt ggcagccgta cgatccacag
    48721 gcaattgatt tgccgtcgtg cctgctttcg ccggatgcgc agcactggct gggcaccgat
    48781 cacttaggtc gcgatatttt ctcgcggctg atggcagcga cccgcgtgtc gctcggttcg
    48841 gtaatggcct gcctgctgct ggtgctgaca ttagggctgg ttattggcgg cagcgccggg
    48901 ttgattggcg ggcacgttga tcaggccacc atgcgcgtcg ccgatatgtt tatgaccttc
    48961 ccgacctcga ttctgtcgtt ctttatggtt ggcgtgctcg gcaccgggct gaccaacgta
    49021 attatcgcca tcgccctgtc gcactgggcg tggtatgcac gcatggtgcg cagcttggtg
    49081 atttcactac gccaacgcga gtttgtgctg gcgtcacggc tttccggtgc gggccatgtg
    49141 cgggtgtttg tcgatcatct ggcaggcgcg gtgatccctt cgctgctggt gctggcaacg
    49201 ctggatatcg gccatatgat gctgcacgtc gcggggatgt ctttccttgg cctcggtgtg
    49261 accgcgccga ccgccgaatg gggcgtgatg attaacgacg cgcgccagta tatctggacc
    49321 cagccgctgc aaatgttctg gccggggctg gcgctgttta tcagcgtgat ggcctttaac
    49381 ctggtgggtg acgcactgcg cgatcatctg gatcctcatc tggtgacgga gcacgcacac
    49441 taatgccgca acagattgaa ctacgtaata tcacgctaca ggccgcgcag ccgctggtac
    49501 acggtgtatc gttaaccctg caacgcgggc gcgtgctggc gttagtcggc ggtagcggca
    49561 gcgggaaatc attaacctgc gccgcgacgc tgggcatttt gcccgctggc gttcgccaga
    49621 cggcggggga aattttagcc gatggcaaac cggtttcgcc ttacgccctg cgcggcatca
    49681 aaattgccac catcatgcag aacccgcgca gcgcctttaa tccactgcac accatgcaca
    49741 cccacgcgcg ggaaacctgc ctggcgttag ggaaacccgc cgatgacgct acgcttaccg
    49801 ctgccataga agcggtgggg ctggaaaacg ccgcgcgcgt gctgaagctg tacccgttcg
    49861 agatgagcgg cggcatgttg cagcgcatga tgattgcgat ggcggtgctg tgtgaatcac
    49921 cgtttatcat cgccgatgaa ccgaccaccg acctcgacgt ggtagcacag gcgcgcatcc
    49981 tcgatctgct ggaaagcatt atgcaaaaac aagcgccggg aatgctgctg gtcacccatg
    50041 atatgggcgt tgtggcgcgt ctggcggatg acgtggcggt gatgtcacaa ggtaaaattg
    50101 tcgaacaggg cgatgtagaa acgctgttta acgcccccaa acatacggtg acgcgcagcc
    50161 tggtttccgc tcatctcgcc ctctacggta tggagctggc atcatgacct tacttaacgt
    50221 ctgcggcctt tcccatcact atgcccacgg tggatttagc ggaaaacatc aacatcaggc
    50281 ggtgctgaat aacgtttccc tgaccctgaa aagcggcgaa accgtcgccc tgctgggacg
    50341 cagcggctgc ggaaaaagca ccctcgcgcg gctgctggta ggcttagagt ctccctcaca
    50401 agggaatatt agctggcacg gcgaatcgct ggcgaaactt aatcgcgccc aacgtaaagc
    50461 cttccgccgt gatattcaga tggtgtttca ggactccatc agtgccgtga atccgcgcaa
    50521 aaccgtgcgc gagatcctgc gtgaaccgat gcgccacctg ctatcactga aaaaatccga
    50581 acaactggcg cgcgccagcg aaatgctgaa ggcggtcgat ctcgatgaca gcgttctcga
    50641 caaacgccca ccgcagttaa gcggcggcca gctccagctc gtctgcctgg cacgcgcgct
    50701 ggcggtcgaa ccgaaattgc tgattctgga tgaagccgtt tctaaccttg atctcgtgtt
    50761 acaggcgggc gtcattcgcc tgctgaaaaa gctacaacaa cagtttggca ctgcctgcct
    50821 gttcatcact catgacttac gcctggtgga acgcttttgc cagcgagtga tggtgatgga
    50881 caacggacaa atcgccgaaa cccaggcggt gggagataaa ttaacctttt cctctgacgc
    50941 cggacgtgtg ctacaaaacg cggtattacc cgcattcccc gtgcgccgtc gcacctcaga
    51001 aaaggtttaa cgccaatgca acgagtcacc atcacgcttg atgacgattt actggagacg
    51061 ctggacagcc tgagccagcg tcgtggttat aacaaccgtt ccgaagctat ccgcgatatt
    51121 ctgcgtagtg ccctggcgca ggaagctacc cagcagcacg gcacacaagg tttcgcggtg
    51181 ctgtcgtatg tgtatgaaca cgaaaaacgc gacttagcca gccgtattgt ttccacccag
    51241 catcatcacc acgacctctc cgtcgccacg ctacatgtgc acatcaacca cgacgactgt
    51301 ctggaaatcg ccgtgttgaa aggtgacatg ggtgacgtgc agcattttgc cgatgacgtt
    51361 atcgcccagc gcggcgtgcg gcacgggcat ttgcagtgcc tgccgaagga agattgagtt
    51421 atttctaaaa caaggaaaga aaagatatta actgaactta atttataaaa ttaagttcag
    51481 ttgcatagat agatcctgta aaggcagata ttctgcgtaa tgtacgtcgc taaaatttat
    51541 aaatgattca aacgctcatt cagtgtttca ataatatagg tattaacaga gacttgttgc
    51601 tgcgcagccg cattattaag gcgttcactc agcgattctg gatagcgtaa cgtaaaggtc
    51661 ttaatctttt ctgtacgcgc atacggctca atacccgcag ctttacaatc ctcaagatac
    51721 tctcgcaggg aaagctctcc ttctttttgc agcccctgaa tactgtcaga gacaaaatca
    51781 caataacctg acaagcccag gaatttgcca cgaaatgcat tcagttctgg cacgtaggta
    51841 ataacagctg gttgcccggc aatctccata gagtttggcg tcttcagttt gatcatggtt
    51901 ttacccctat acttagtaac cagtcacaca cgctttctac agcgccttta tcggtatctg
    51961 gcgacggatg aggccgatgg aaacacgcaa cgctcatatt tagtatgaac ttgcaacgcg
    52021 aacctcttcc ttccttaatt tctccgccta atgctttaac cagtgactct atatcagacc
    52081 acttgatccc ttgaggaaca ggagttttaa atatttgatc caatgtattt ttttgtttct
    52141 tccgtaaagc ctgtaccttg gttctcatgg catgtcctta tgacttcact ttatgacatc
    52201 acagtatgaa gtcaagtttc ccttccttac gccattgtcc caatcgtctt cctgaatcgc
    52261 agcagtgcga tggtgaaaaa cgcgccgcca attgccatca gcgtcaggaa ctgcggccag
    52321 acgatttcga atccggcacc ccggtagagg atggcctgcg cgaggctaac aaagtgtgtc
    52381 gtcggcatgg tcagcataat gtcctgcacc atctgcggca tactttcgcg cggcgtggat
    52441 ccaccggaaa gcatttgcag cggcagcagc accagaatca ccagcagccc cagttgcggc
    52501 attgaacgcg ctatcgtccc cataaaaatg ccgattgacg tggtggcaaa cagactgagc
    52561 gccacgccca gcataaacag cgggatcgag ccttcaatcg gtacgcccag tacacctttc
    52621 accatcagca ccagcgataa tcccgatacc accagcacca ccagccccat cgaccagatc
    52681 ttcgccatca tgatctcaaa cggcgttatc ggcatcacca gtaaatgttc caccgtgccg
    52741 tgttcacgct cgcggatcag cgccgatccg gtcaatacaa tcgccagcat ggtaatgttg
    52801 ttgatgatcg ccatcacccc gccaaaccac gcgggatcaa ggttcgggtt aaagcgcatc
    52861 cgggtttcca gcgataccaa cggttcgctg ttatcacggt agcgcgcgac aaagctgttc
    52921 acttcaccgt tgataatatt ctggatatac ccattgccgg taaatgcctg gctcatgcgc
    52981 gtggcatcga cgttcacctg aatatccggc tggcgtccgg cgaggacatc acgctgaaaa
    53041 ttaggcggaa tatttatcgc gaaggtatag cgtccggcgt ccagtccggc atccatctca
    53101 tcggcggtga tcatctccgg tggcaaaaac cacggacgat agaagctgtt aacgatccgg
    53161 ttcgataact gcgattgatc catatcggca atggcgatcg gcgcgaggtt caacgatcct
    53221 ggcgtaacgg tcgctgacga atacaccgac accgtaaacg agaagacaat cagcgtcagc
    53281 atcgctttat caccgagcag actgcgcaac tctttgatac ccagattaaa aatattgcgt
    53341 aaatggcgca tcatccctcc tgttttttca gcagcaggat gcttaagccc atcaccagcg
    53401 ggatggctat cagcaacggg ataaaaagtt gccacaaatc agtcagatcc agcgctttcg
    53461 agaacgttcc gcgggcgata gtcagaaaat gactggtcgg gtaaacctcg ccgatccaac
    53521 gtccaggccc ttccagcgaa gctaccggat cgatcatccc ggaaaactgt gtcgccggga
    53581 tcaatgtgat aatcgccgtt ccgaaaatgg cggcaatctg gcttttcata aaggtggaga
    53641 tcagcagccc cattccagtg gcaatgatga tatacagcag cgccgccaag gtgagcgtca
    53701 ggaaactgcc tttatgcggt acgccaaaca caaacaccga caggccgcag agcaggaaaa
    53761 agttcagcat ccccagcgcg atgtacggca gctgtttgcc aagcaaaaat tcgctacggg
    53821 tggtgggggt cacgtaaagg ttgataatcg accctagctc tttttcccgc accacgctaa
    53881 gggcgcttag cattgacggg atcatcatca gcagaagcgg gatcaccgcc ggaacaatcg
    53941 ctggcaggct ttttacgtcc gggttatagc gataacgcgt ctcaatgttc atcagcccgc
    54001 tttggctggc gggtgtcgat tgtcggctcg ccacatcctg aagccaactc tggtgcattg
    54061 cctgcacgta accttttacc gtttcggcac gactgggcat cgctccgtcg atccagacgc
    54121 cgagttccac aggcgtacca cgcgcgatat cgcgcccgaa attgggcggg atctcaatgg
    54181 ccaccgtgat atcgcccgca cgcatccgac gatcaagctc gtcataactg gtgagcggcg
    54241 gctgttcgat aaagtaacgg gaaccggaga ggttgagtgt ccacgcctgg ctactgacgg
    54301 tctggtcgcg gtcgagcacc gcaaagcgca ggttttccac atccatactg atgccgtaac
    54361 ccattatcag catcaggatc accgttccca tcagcgccag cgtcgaacgt actggatcgc
    54421 gtcgcagttc cagcgcttcg cggcggctgt agctaaacag acggcgcagg ctaaatccct
    54481 gacgcggcgc gtgggtggtg tcgtgtacca cgggcggcgc ttcggcttcg ttgctctgcc
    54541 ctgccgcttc ctgcaaatag gcgataaatg cctcttccag actggcggct ccgcgtttct
    54601 caaccagttc ctgcggtgta ccgctggcaa gcacttttcc ggcgtgcatc agtgagatgc
    54661 ggtcgcaacg ttccgcttcg ttcataaagt gggtggagat gaagatagtc actttgtcct
    54721 ggcgcgagag atcgaccatc aactgccaga acatatccct cgccaccgga tcgacaccag
    54781 aagtaggctc atcgaggatt aacatctccg ggcgatgaat caccgccacc gccagcgaaa
    54841 gccgctggcg aatgccgagc ggcaatgact ccggcagaac atcttcaacg tcgttgagct
    54901 taaaacgctc gctcatttca gccactcgcg cgggaatttc cgcttccggg atgtgaaaca
    54961 aacgggcatg taactccagg ttttgccgca cggtgagttc gttatagagc gaaaacgcct
    55021 gcgacatata gcccacacga cggcgggtat cgatatcttt tggatcaacc ggttgcccaa
    55081 atagccacgc ctcaccttcg ctggcgggca gcagtccggt aagcattttc atggtggtgg
    55141 atttaccgca gccgttcgaa ccaaggaatc caaaaatctc cccgcgtgga atgcggaaat
    55201 tgacgtgatc aacggcaacg aaggaaccaa aacgcatggt cagatcgcgc gcttcgatgg
    55261 caatctctgc gttttcaggt tgatacggtg ggatcactac cgcctgatgc gcctggcgtt
    55321 gcgcttgcgg taacagatta ataaatgctt cttccagcgt ggcgctttgc gtttgctgcc
    55381 gcagctcttc ggcgctgccg gttgccagca cttctccggc attcatcgct accagccagt
    55441 cgaagcgttc ggcctcttcc atataggcgg tggcgaccag cacgctcata ttgctctgcc
    55501 gctggcgaat actgtcgatc agatcccaga actgggcgcg ggagagcggg tcaacccccg
    55561 ttgttggctc atcaaggatc aacagttccg ggtcgtggat taacgcgcag cacagcccaa
    55621 gtttttgctt catcccgccg gagagtttac ctgccggacg atcgcgaaac ggtgctaacc
    55681 cggtgctggt cagcagctca ttaattcgcg cttcccgctc cgctttatcg tgaccgaaca
    55741 ggcgagcgaa aaaatcgaca ttttcataca ccgacaaggt gtggtagagg tttttgccca
    55801 gcccctgcgg catccaggcg atgcgcgggc agacgtctcg gcgatgcttc gggtcgtgca
    55861 tatcgccacc cagcaccacc acattgccct gctcaatgac gcgggcaccg gaaatcagtg
    55921 acaataaact cgacttcccg acgccatccg gaccaatcag cccgaccatg caacgggccg
    55981 gaatatcgag agtgatattg ttcagcgcaa cggtttttcc ataatgctgg ctcacgcccg
    56041 ccagttgcgc gacaggcggg acgggaacca gttccagatg cgtcattgcg gcaacctcac
    56101 cacgaggtcg tcaggccacg gaagttcttc attcacccgc acccacgcta cgcccggtaa
    56161 accggttttg acatattcca gatgctgctg gagtaattcc ggtgggatac gcgctttgac
    56221 gcggaacatc agtttcagcc gttcatcgct ggtttcgacg gtttttggcg tgaactgggc
    56281 gacactggcg acaaaactga tggttgcagg aatacgcaga tctggcgcgg catcgaggat
    56341 cagccgggct tcaccgccca gtttcagcgt gcccgcctgt tcggttggca ggaagaaggt
    56401 catatagacg tcgctgagat cgaccatatt cagcacccga ccgcctgccg ccagcacttc
    56461 ccctggctcg gcaacccgat actgcacgcg tccgtcacgc ggggctttca gttcactgtc
    56521 atcgatatct gcggcaatgc gccgttcagt ggcttgcgct gcttcgaccc gagtttgcgc
    56581 ctgaatgata ttggtgcgtg ctgcttctat agccgcttta gaagccgaaa cctgggcttt
    56641 cgctgattcc agcgcagctc gggcgctctc agcggcggcg cggtcgtcat ccagctgttg
    56701 cgcagaaata gcccctcgtt gggcgagcga gcgggaacgc gtatgacgtt tggcaacaga
    56761 gtccagttcg gcctggcgtt gattgaccag cgactgtgcg gcacgagttt cactttgtcg
    56821 ttgctccagc aaagcctgcg cggcggcaac ggcgctttgt gcctctttga tttgcgcgat
    56881 ggcttccagt cgctgttcct gcaacacgcg agtatccatc ttcgccagca cttcgccttc
    56941 gcgaacaaac tggccttctt tcaccagaat ggtgtcgata cgcccggcaa ttttgctggc
    57001 gatatccact tccgtcgctt caatgcgccc attgctgaca gcaaagcctt ccggcacacc
    57061 cgccgggcgc aacagccacc acgccacgac ggcagccacc gccagtgccc cgacaaccca
    57121 ccacgccaga tggcgcttac tcttatccat aatcgacccg ccataatccc tgtaagcaaa
    57181 cgacgcgtgt tacaactgcc gccgaacgac gttcagcgga ttcttccatc cgggataaga
    57241 aggaaacgac aaatacgacc agcgcgcaga gcattgagcg ccaacgggga aggcttagtg
    57301 aaaacgggta gtaatgttgt catcatcagc attcctggcc gtaaatgaaa cacgcatact
    57361 cataagtgtg atctataaaa ctggctgaca aaacggtatc catcctgttg gatcgcattt
    57421 cacgcctgtt cagttgcagg aacagagcat tcctttttac gttttatcaa cagtctcaac
    57481 cagatagtaa cattaatatt catgttgtct atggttcaga gctgtaacat tgtgcgcctt
    57541 ctaatcaaag gcataagtcc atttctgttt ttttcatgct ggtaagactt ccagggagtg
    57601 gttatctctg gccctgcacc agggttaatt tggcaattat gcatgctgaa cattcttcca
    57661 gcatgtttac tgtcacgggc gttaagacct aaaactgtcc ccgtttcacc tatgcgttct
    57721 ctcccaccat ttccgttatg tgcaacaact tgatttatac ctaacatttg tgaacatgct
    57781 aatagtgctt caagtttagg tctgtaatct gtcgaaacag aaaaataacg attaaaggat
    57841 cgacaagaga tagcccggct agttttaaac tttttgccat taattatagc atgatgcttt
    57901 ttattaataa tttccgctaa ttcgaccgga tcgagagcat tggtcatttc atcaatagag
    57961 acagttatcg gtcctataat gtaacgtttt cgctgatcat caaaagttat cccatgatga
    58021 tttgccatta ttttgtattt ggggtcatag aaacatacag gataatcttt tatcccgtcg
    58081 taggtatcct catcaagtgg tttcatgcgc gtgaagtttt gcatatagtt accattgcaa
    58141 ttcgtttcat gatttccagc taatacatga acattttcat tgatactcat tatgcgtttt
    58201 aacaaatcga tgataaattg atcgccacag atattactaa atcgatcgcc ggtttggtcg
    58261 ccaagaaacg tacatggcgt gactgcgtct ttattaataa ccaaataagg ctgaagactt
    58321 tctaaaacat tatctcttct gttcagtata ctgattattg tattaacatt agtgtcaagt
    58381 tgctcattaa agttctccct tagcaatacg ttgagaactt catcttctat attaagcaac
    58441 tggctaagga aattttctcc ttcagccatg attcccaaat gtcctgttgc aaaaagcgcg
    58501 tacaaaactg caccgactga gccatcggta tcgccaaaat aggtcggcct ggtacttgtg
    58561 ggatcaacag gtgaacaatt ggagaaatag gttctgtcag cgaactgggg ttttataatt
    58621 gtttcgaatt taatatcaaa tacattgtat tgcgatattt cccagttcca gatgggtttg
    58681 tacgactcag taactgcaac atcattagca ttatcattta ctgtcatatt attaagtaaa
    58741 taaacaatgc tgcaccaatt aatatcggta ttatcaagag ggcaaccgta atttttacta
    58801 ttaattgtca caataaactg ataacagtta ttcctacgta gaatatgagc gcgcatgccc
    58861 gatagcatac tatatttttc tgaatgattc gtttgtatgg taaatgaaat tttgtcatct
    58921 tgaatagatt gttggaaacg ttctatttgc caatcatcaa ttttttccac aacttcaccg
    58981 agcattgtcc tggcaatact tgatatatca tcacctatac catttgcaat atacccaaac
    59041 agttctttag tgaagttggt aacatgctct tgcaccatat catttgttgc gattgtcgtt
    59101 gtactgccat tagtgcctgc cacagttcca attttcatct tttacctcat attattcacc
    59161 gcgaggaata catagttaac attttatctt atgacataca cctcatattc gatggggaaa
    59221 taattatatt ttgcacaaat cattcagcct taaagcataa tatgaaattt cttatctcat
    59281 agcagagaaa gtccctaaat attgtagagg cgtcatcagt acatacctct acaatcgatt
    59341 aagaaaacct atgaaatact ggcaaaatta aaaagagata ccacagcact catgaagttt
    59401 tattgaggtt gaaaacaact aagccctggg acaccttttt tatcaccacg ttcgttaggt
    59461 gctatcttcc aagaatttag ccccatggta taattatcaa ttttttgatg ctggtctacc
    59521 cctaatccat aatggccaaa tttctgttta gtttttttga gcgtctgtcc acatttgaag
    59581 atatcttcag gttttggtct gaaagcagta gtgttattaa aataattttc atatacaggt
    59641 gtgggtgcta tagcattgct tctaattaga tggagaagat gatcatccat gtgttgttta
    59701 tgttttttat tacatatatt tgctaattcc tcaggatcaa ttgggtcttt catctgatta
    59761 aatggaacct gcaaagcacc gagacaataa cattgatttt cttcatttct tattattccg
    59821 tgatgactgg ttaaaacttt agtttctgag ttataattac aaacatcgag cgttttaatt
    59881 aagttatacg tatcaccggc agagagttta taattttcta gtcgtgcagt atagttacca
    59941 ttaaaattga tttcatgatt tccagccaga acgatgacat ttttattaat acggctatct
    60001 ttattaccct ccatatttcg catagaattt agcaaagtaa ggatatattt atcaccaaaa
    60061 atagtactaa atctatcgcc tgtatgatcc ccaaggaaga tacaaggggt tatcgcatcg
    60121 tcctttaaaa aaacatgttg atgcaactca ttcaatatca tgtctctcgt atttaataaa
    60181 ttgtagagca atttattatt tttatgtatg tatgcagatg aagccatgtc ttcgtgtttt
    60241 aataattcac ataataaact ccaacccttt tccctgatgc ctatatgccc tgaaacgagc
    60301 aatgaataaa gtacagcgcc tactcttcca tctgtatcgc caaaataagc cgtgttggca
    60361 ttttgtgggt taatcacacc acattgttta taaaagtcta ttttagaaaa cacagattta
    60421 acatagctat caaacttatc cttaggaaac gtctctttag agacatccca acgccagtca
    60481 gctttaaaat taccgctaag aagagtaaag aaattttcag gtatgataga aagcccatta
    60541 actttataaa ttatgtctga tttatctccg gagaaattaa taaaataata accatcctta
    60601 cactcaatct taactgtaat gggagacgat aattttatca catggttatt gtctggtata
    60661 attgtaaaga taaacgtgtt agtagtatta tcggcaaaac tattatattc ttgaatatct
    60721 atagatggct cactattgta aaaccatatt ttctccatct gaagactatg ttcatttacc
    60781 ctccagtgac catgttgcag gaaatagatg atatgcatgc caaaatcgtt atctatttgc
    60841 gaatcattat ccagcttatc aatttcaact atctttgaac gatgtgtttt atcaaccttg
    60901 ctatggtgtt gatgattatt aactttatct gaatgcttat ctttatggtt tgaagggatg
    60961 tacttaaata tattgtcaat tgctttcata aataacccgc gatatttttg acctgttagt
    61021 aaaaaataaa tgatacatgt ctgaaagaca tatttacgac gatataaaac aataatactt
    61081 atatgttcgc tgattttaag caccgagact atccggtctt ttactaaata atggaaaaca
    61141 atccatccct ggaagacaat tgtaaaaaaa acaacagaat aagaattaat cttaggataa
    61201 atatttattt atcatggcct tttgaccgct aaaaaatggc actcggcaca gtaaaccccg
    61261 ctatagttat gctatgcatt tgtctttaat gatatctcgt cgttatatta acaggctgaa
    61321 attatcatta atgcacattt caatattagc agggatacct gaggagttta aagtgaacat
    61381 atatatcggg tagcttttca aattaatccc tttgattata ggcttaattt gcatcgcgtt
    61441 aggtggcttt gtgctggaaa gttcagggca acgtcacggg tcatgtgctg atttctctgg
    61501 cggccatatg cctggcatta ttcactaccg catttattat catttcgcaa ctcacgcgcg
    61561 gcgttaatac gttttacaat acattgttcc ccattattgg ctatgcgggg tcaattatca
    61621 ccatgatatg gggttgggca ctgttagcag gtaatgatgt gatggcagac gagtttgttg
    61681 ccggccatgt tatttttggc gttggtatga ttgccgcatg tgtatcgacg gtggcagcgt
    61741 cgtccggtca ctttctgctc attcccaaaa atgcagcggg gagcaagagc gacggaacac
    61801 cggtacaggc ttattcttca ttaatcggta attgcctcat tgccgttccc gttttactca
    61861 ccctgctcgg tttcatttgg tctattacgc tgttacatag tgccgacata actccgcatt
    61921 atgtcgcctt gggttaaccg caatctgtgc ctgtctaatt ggcctagtgg ccacaatcgt
    61981 ccatcaaaca cgtaatacgt tctcatccaa agagcactgg ctgtggtgtt attgggttat
    62041 ttttctcggc tcagtcacgg tgctgcaggg gatatacgtc ttagtcagtt ccgatgcaag
    62101 cgcccgactg gctcccggca ttattctcat ttgcctcgga atgatctgtt acagcatatt
    62161 ctcaaaagtc tggctactgg cactggtctg gagacgtacc tgttcgttag ccaacagaat
    62221 accgatgata ccggtattta cctgtctgtt ttgcttgttt ttgtcatcat tcctcgcgga
    62281 aatcgcacaa actgagctgg actactttat tccatctcgg gttctggttg gattaggcgc
    62341 agtatgtttt acattgttct ctattgtttc aatattagag gccggttctg cgaaaaaaaa
    62401 ttaaaattta cataatccca gcgttattca tatcttaacg ctggtttttt acgaacttag
    62461 tgaaataatg tagaatataa aattataatt ttatattgga gcacatgtgt taatgatcaa
    62521 ccccgggata ctaaaaatat agacattatt aaacttgctt agcctcgcat tgcctttacc
    62581 tgttaaagat gcatcgagaa aattgagtct gaagttctca tcctttatct gattttccag
    62641 tcccgaaact ttggccttag cattacctga gatccgtccg ttactatcta ttttgtaggt
    62701 aaaaaaatac gaatgggaaa atttcctcgg tgtatcttca tcagttaagc caacaatcgt
    62761 aaaactaccg ctaccatctt tttttaaaat gactgaaagg ttagcagagt agtataaaga
    62821 atgatctgtt ttagcatttc ttgttaacgt cgacgtacat tcaagagacg ttgtgctacc
    62881 atcacgagcc ttccagagag caaaaaaaca cacctgtagc aatgtgaaaa caagtattaa
    62941 tataaaatac attttcttat tagtcatata atagcacctc tgaacaaatt gcataagttt
    63001 cgtcattaga ggattttata catcttgata aataccgctg cccttcccca agcaaataat
    63061 gcatactatt tacatcatat ataaaatttg cacctggcac acagggtaaa tgctttttcg
    63121 aaatttcagc ggcctctatt tgagatttag cgatgccaat ctcgttaaga ttctctgaaa
    63181 gatataaatc acaggtgtca atcttacctg atttaaccaa tggataatcc gttatactgt
    63241 aggtatcttt aaaagccacg gcaaacattt caataataac aattactacc acaagcgaaa
    63301 tcaaaatgcg aaaaagagga atactccttt tgcgctctac tttttcaaca acatcttctt
    63361 gtaaatgaga atcatcataa gattgtttac ttattgttat gttttcactg atgtaaaagc
    63421 ctactttggg tatcgtaaga atcacatttt cttcaacacc aatattttta aaacttttgc
    63481 gtaacaggga tatatattgg ttcaatgtgt tgtttgacag atcaaatgaa aatctctcca
    63541 aaacgctatc aatgattgag ttcctgtcaa ccgctccacc attactttcc agtaacactg
    63601 ccaaaataca ccccgccgta gctggaaaat ttatctcatc ctgggtattt acattcgaga
    63661 gcgagcattt gttcggatca aaaataatcg cgtcattgat aatgaaaaaa catctccgct
    63721 ccatgcaggc aacaacacaa aataagattt tgtcatttta gcctattcct ggtggaactg
    63781 acacaagaaa ctaaaataca aatataaccc tgaaaaagtc atataaaact tatatccaca
    63841 gaataaaaaa tcaaacatgt cagttttatc aatataaagt atcgcaaggc gaaaagtgat
    63901 gcaagaatac tcctaagtaa atgaaagcgc accacatgaa attatcaaca catgtaattt
    63961 catcattagg tgctatcgaa aataactcag gagctactta agatgaataa tgtcttactc
    64021 atgagacccg atgactcaga atttatgata cctgatgact ggtcagggct aaacgcaaaa
    64081 ggcattacat acgaacaaga taatagagtg acagagtctt ttctgccttt taaatctttt
    64141 tattgttcac cggtgtgcac gacagttgaa ctcatctcat aaaataattg ttttgctatg
    64201 ggtatccgct accttctgga aaagaaaaga gacttaaggc taaatcactt taaagccttc
    64261 tctcataaat gcgagacaaa acctgacgtg ttaattatga ccgggtgcac cgtcgctgat
    64321 acttatagta tattctcacg cattgttgaa gaaaatataa tgaaaacgat gacacctatc
    64381 gtcatcatta ctgggcatca ctcagaagat ctttgttctc tggcatcgaa actgggagga
    64441 gttaaaataa tatcaccgac agaaaagatt gcaaaaatag aaaacgctat caatgatgct
    64501 tttaacagca agcctaaaaa aataagattt ttaacagata tactctctcc tcaacaaatt
    64561 gagaccatga aaatggttgc gcagggtaga tccgttaatg agatttcagc cgcaatgaat
    64621 attagcatca agaccgtgta tgcacataaa gttcgtgcta tcaataagct gggaattaag
    64681 aaaaaagttt atgaagcgct tttttataaa gcgctaattc agttcaactg atgtaaaaat
    64741 caccctcgct taataaataa tatattctat gtcttttttt ataagttaaa aaaggcggga
    64801 tatcaaatcc cgccatgaaa ttaactaatc atctgcatta ttctgcgtag atataagcct
    64861 gggtacggat ggtgtaagag tctggctcaa caacctgttc tttcatatgg gaaacgacgc
    64921 gataggcacc acctgttcca gtatttttca gcatcatata acgcttaccg ccaatctctt
    64981 cggaggcagc tgaaggagga tcctgctcaa gcgttgtcag ggaaactttc agtttatggt
    65041 ccgggttatt gtcaccagaa attactgcat ccgctgaagt actgctctcc gcataaatat
    65101 catccgggaa agttacagca tagtcgtggc tctgcccatc agatgcgcca acaatacctg
    65161 ttgccaaagg cgtccatgca gttaacgtac cgctatatac ccctttcact ggttgaatgg
    65221 tattattaac aacgataggc ttggtcaaag taagattagc tgacgcttca gtaccctggc
    65281 ttactaaagt tcccgcctga gaagcaaaag ctgtccccgc cagcatgatt gctaacaatg
    65341 ttttcttcat tatattttct ccatgatttt catttacact gcaaaatgca gtttctttct
    65401 ttgcatttac gtgctatcgc gaaaatgctt ccaccaaata atggaaacat cttcacaaaa
    65461 ataaattatg agtttcttaa tttagaaact ataaacatgt cgccgctatc tggtataacc
    65521 ctgatgcact tttcttcgca ggaagttggt attttgcgat acaggattga tctttagaat
    65581 aaccccattt cacattaaac tcgccttccg cactcaagcc agaaacataa gcaataccat
    65641 cttcacctac aatgccggcc atattttcag tgtctttgct ataactaatc acagcgccga
    65701 aggggatggg tttaccctct ttctgtttta aggttactaa tcctttatcc ccgctcttca
    65761 ctgccaatgt cgcaggcaca acggctccag ctgtcggtac aaccaattga ctagtttgtg
    65821 gtaagtcgta attcttacct atcgtttttt catccagaat aacatcgtct cttcggtatg
    65881 gctgcactgg cagtattgca tacccacgaa aatcagtact cagtcccggc cagccattaa
    65941 cgttaacccc cgttgctccg ggtgcctcta taagggctaa tgtcccatcc acattttgac
    66001 caaatgtaag tccatccctt gtaataagta tcccaccgct taaatttgca cctagctgtt
    66061 tcatgttttc gctggagctg taatggctac taaactgacc atatgatcca tttttgccaa
    66121 cttgcacaat gttcatcgtg ctcttcggtt gtgactgatg agcgttagtt atatttgtcg
    66181 ctacatccca ccatacacca ccactattct gacctgataa tgaaacctga tagttattac
    66241 cctgctctcc cgaacgactc atctgtgaag acgcataaat ttgattttcg tcacccagcg
    66301 ataatggcac tgaaataccc acgcttaatt tccgatctac tttccattta tgcgatgtca
    66361 gcaagtcgtt ttttgcccag ttaatggaga atgttatgcc tctccatacc gtactataac
    66421 tcaggctggt agaccgcgtg tttcctgtgc cccgggaaga attatacctc gtggtattga
    66481 agttcaaatt cccgatatta ccaaggggct gggataagga tatcgtagag ccatcgtgac
    66541 tattcttatc tctttttctg ttccgttcat cctgtgcact tactgcttca gtcagtgtgg
    66601 cataacctga tgcataacgg ttcgtggtta taga
//
LOCUS       DAJTRC010000010        66032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.010, whole genome shotgun sequence.
ACCESSION   DAJTRC010000010 DAJTRC010000000
VERSION     DAJTRC010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 66032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 66032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.010"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(128..607)
                     /gene="folK"
                     /locus_tag="OO357_000701"
     CDS             complement(128..607)
                     /gene="folK"
                     /locus_tag="OO357_000701"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HCR5885752.1"
                     /translation="MTVAYIAIGSNLASPLEQVNAALKALGDIPESRILAVSSFYRTP
                     PLGPQDQPDYLNAAVALETSPAPEELLNHTQRIELQQGRVRKAERWGPRTLDLDIMLF
                     GNEVINTERLTVPHYDMKNRGFMLWPLFEIAPELAFPDGETLREVLHTRAFDKLSKW"
     gene            complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="OO357_000702"
     CDS             complement(604..2022)
                     /gene="pcnB"
                     /locus_tag="OO357_000702"
                     /EC_number="2.7.7.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000174644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polynucleotide adenylyltransferase PcnB"
                     /protein_id="HCR5885753.1"
                     /translation="MTLPRCTIFTRVANFCRKVLSREESEAEQAVARPQVTVIPREQH
                     AISRKDISENALKVMYRLNKAGYEAWLVGGGVRDLLLGKKPKDFDVTTNATPEQVRKL
                     FRNCRLVGRRFRLAHVMFGPEIIEVATFRGHHEGNVSDRTTSQRGQNGMLLRDNIFGS
                     IEEDAQRRDFTINSLYYSVADFTVRDYVGGMKDLKDGVIRLIGNPETRYREDPVRMLR
                     AVRFAAKLGMRISPETAEPIPRLATLLNDIPPARLFEESLKLLQAGYGYETYKLLCEY
                     HLFQPLFPTITRYFTENGDSPMERIIEQVLKNTDTRIHNDMRVNPAFLFAAMFWYPLL
                     ETAQKIAQESGLTYHDAFALAMNDVLDEACRSLAIPKRLTTLTRDIWQLQLRMSRRQG
                     KRAWKLLEHPKFRAAYDLLALRAEVERNAELQRLVKWWGEFQVSAPPDQKGMLNELDE
                     EPSPRRRTRRPRKRAPRREGTT"
     gene            complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="OO357_000703"
     CDS             complement(2061..2987)
                     /gene="gluQRS"
                     /locus_tag="OO357_000703"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001432381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HCR5885754.1"
                     /translation="MLPPYFLFKEMTDTQYIGRFAPSPSGELHFGSLIAALGSYLQAR
                     ARQGRWLVRIEDIDPPREVPGAAETILRQLEHYGLHWDGDVLWQSQRHDAYREALAWL
                     HEQGLSYYCTCTRARIQSIGGIYDGHCRVLHHGPDNAAVRIRQQHPVTQFTDLLRGII
                     HADEKLAREDFIIHRRDGLFAYNLAVVVDDHFQGVTEIVRGADLIEPTVRQIPLYQLF
                     GWKVPDYIHLPLALNPQGAKLSKQNHAPALPKGDPRPVLIAALQFLGQQAEAHWQDFS
                     VEQILQSAVKNWMLTAVPESAIVNSTFSNASC"
     gene            complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="OO357_000704"
     CDS             complement(3024..3479)
                     /gene="dksA"
                     /locus_tag="OO357_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002209354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HCR5885755.1"
                     /translation="MQEGQNRKTSSLSILAIAGVEPYQEKPGEEYMNEAQLAHFRRIL
                     EAWRNQLRDEVDRTVTHMQDEAANFPDPVDRAAQEEEFSLELRNRDRERKLIKKIEKT
                     LKKVEDEDFGYCESCGVEIGIRRLEARPTADLCIDCKTLAEIREKQMAG"
     gene            complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="OO357_000705"
     CDS             complement(3657..4361)
                     /gene="sfsA"
                     /locus_tag="OO357_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HCR5885756.1"
                     /translation="MEFSPPLQRATLIQRYKRFLADVITPDGRELTLHCPNTGAMTGC
                     ATPGDTVWYSTSDNTKRKYPHTWELTQSQSGAFICVNTLWANRLTKEAILNESISELS
                     GYSSLKSEVKYGSERSRIDFMLQADSRPDCYIEVKSVTLAENEQGYFPDAVTERGQKH
                     LRELMSVAAEGQRAVIFFAVLHSAITRFSPARHIDEKYAQLLSEAQQRGVEILAYKAE
                     ISAEGMALKKSLPVTL"
     gene            complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="OO357_000706"
     CDS             complement(4376..4906)
                     /gene="thpR"
                     /locus_tag="OO357_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HCR5885757.1"
                     /translation="MSEPQRLFFAIDLPAEIREQIIHWRATHFPPEAGRPVAADNLHL
                     TLAFLGEVSAEKEKALSLLAGRIRQPGFTLTLDDAGQWLRSRVVWLGMRQPPRGLIQL
                     ANMLRSQAARSGCFQSNRPFHPHITLLRDASEAVTFPPPGFNWSYAVTEFTLYASSFA
                     RGRTRYTPLKRWALTQ"
     gene            4980..7409
                     /gene="hrpB"
                     /locus_tag="OO357_000707"
     CDS             4980..7409
                     /gene="hrpB"
                     /locus_tag="OO357_000707"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HCR5885758.1"
                     /translation="MSSLPVAAVLPELLTALDCAPQVLLSAPTGAGKSTWLPLQLLAH
                     PGINGKIILLEPRRLAARNVAQRLAELLNEKPGDTVGYRMRAQNCVGLNTRLEVVTEG
                     VLTRMIQRDPELSGVGLVILDEFHERSLQADLALALLLDVQQGLRDDLKLLIMSATLD
                     NDRLQQMLPEAPVVISEGRSFPVERRYLPLPAHQRFDEAVAVATAEMVRQESGSLLLF
                     LPGVGEIQRVQEQLASRIGSDVVLCPLYGVLSLNDQRKAILPAPQGMRKVVLATNIAE
                     TSLTIEGIRLVVDCAQERVARFDPRTGLTRLITQRVSQASMTQRAGRAGRLEPGISLH
                     LIAKEQAERAAAQSEPEILQSDLSGLLMELLQWGCSDPAQMSWLDQPPTVNLLAAKRL
                     LQMLGALEGERLSAQGQKMAALGNDPRLAAMLVNAKSDDEAATAAKIAAILEEPPRMG
                     NSDLGVAFSRNQPAWQQRSQQLLKRLNVRGGEADSSLIAPLLAGAFADRIAHRRGQDG
                     RYQLANSMGAMLDADDALSRHEWLIAPLLLQGSASPDARILLALPVDIDELVQRCPQL
                     VQQSDTVEWDDAQGTLKAWRRLQIGQLTVKVQPLAKPSEDELHQAMLNGIRDKGLSVL
                     NWTAEAEQLRLRLLCAAKWLPEYDWPAVDDESLLATLETWLLPHMTGVHSLRGLKSLD
                     IYQALRGLLDWGMQQRLDSELPAHYTVPTGSRIAIRYHEDNPPALAVRMQEMFGEATN
                     PTIAQGRVPLVLKLLSPAQRPLQITRDLGAFWKGAYREVQKEMKGRYPKHVWPDDPAN
                     TAPTRRTKKYS"
     gene            7605..10139
                     /gene="mrcB"
                     /locus_tag="OO357_000708"
     CDS             7605..10139
                     /gene="mrcB"
                     /locus_tag="OO357_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glycosyl
                     transferase/transpeptidase"
                     /protein_id="HCR5885759.1"
                     /translation="MAGNDREPIGRKGKPTRPVKQKVSRRRYEDDDDYDDYDDYEDEE
                     PMPRKGKGKGKGRKPRGKRGWLWLLLKLAIVFAVLIAIYGVYLDQKIRSRIDGKVWQL
                     PAAVYGRMVNLEPDMTISKNEMVKLLEATQYRQVSKMTRPGEFTVQANSIEMIRRPFD
                     FPDSKEGQVRARLTFDGDHLATIVNMENNRQFGFFRLDPRLITMISSPNGEQRLFVPR
                     SGFPDLLVDTLLATEDRHFYEHDGISLYSIGRAVLANLTAGRTVQGASTLTQQLVKNL
                     FLSSERSYWRKANEAYMALIMDARYSKDRILELYMNEVYLGQSGDNEIRGFPLASLYY
                     FGRPVEELSLDQQALLVGMVKGASIYNPWRNPKLALERRNLVLRLLQQQQIIDQELYD
                     MLSARPLGVQPRGGVISPQPAFMQLVRQELQAKLGDKVKDLSGVKIFTTFDSVAQDAA
                     EKAAVEGIPALKKQRKLSDLETAIVVVDRFSGEVRAMVGGSEPQFAGYNRAMQARRSI
                     GSLAKPATYLTALSQPKIYRLNTWIADAPIALRQPNGQVWSPQNDDRRYSESGRVMLV
                     DALTRSMNVPTVNLGMALGLPAVTETWIKLGVPKDQLHPVPAMLLGALNLTPIEVAQA
                     FQTIASGGNRAPLSALRSVIAEDGKVLYQSFPQAERAVPAQAAYLTLWTMQQVVQRGT
                     GRQLGAKYPNLHLAGKTGTTNNNVDTWFAGIDGSTVTITWVGRDNNQPTKLYGASGAM
                     SIYQRYLANQTPTPLNLVPPEDIADMGVDYDGNFVCSGGMRVLPVWTSDPQSLCQQSE
                     MQQQPSGNPFDQSSQPQQQPQQQPAQQEQKDSDGVAGWIKDMFGSN"
     gene            10359..12602
                     /gene="fhuA"
                     /locus_tag="OO357_000709"
     CDS             10359..12602
                     /gene="fhuA"
                     /locus_tag="OO357_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrichrome porin FhuA"
                     /protein_id="HCR5885760.1"
                     /translation="MARSKTAQPKHSLRKIAVVVATAVSGMSVYAQAAVEPKEDTITV
                     TAAPAPQESAWGPAATIAARQSATGTKTDTPIQKVPQSISVVTAEEMALHQPKSVKEA
                     LSYTPGVSVGTRGASNTYDHLIIRGFAAEGQSQNNYLNGLKLQGNFYNDAVIDPYMLE
                     RAEIMRGPVSVLYGKSSPGGLLNMVSKRPTTEPLKEVQFKAGTDSLFQTGFDFSDALD
                     DDGVYSYRLTGLARSANAQQKGSEEQRYAIAPAFTWRPDDKTNFTFLSYFQNEPETGY
                     YGWLPKEGTVEPLPNGKRLPTDFNEGAKNNTYSRNEKMVGYSFDHEFNDTFTVRQNLR
                     FAENKTSQNSVYGYGVCSDPANAYSKQCAALAPADKGHYLARKYVVDDEKLQNFSVDT
                     QLQSKFATGDIDHTLLTGVDFMRMRNDINAWFGYDDSVPLFNLYNPVNPDFDFNAKDP
                     ANSGPYRILNKQKQTGVYVQDQAQWDKVLVTLGGRYDWADQESLNRVAGTTDKRDDKQ
                     FTWRGGVNYLFDNGVTPYFSYSESFEPSSQVGKDGNIFAPSKGKQYEVGVKYVPEDRP
                     IVVTGAVYNLTKTNNLMADPEGSFFSVEGGEIRARGVEIEAKAALSASVNVVGSYTYT
                     DAEYTTDTTYKGNTPAQVPKHMASLWADYTFFDGPLSGLTLGTGGRYTGSSYGDPANS
                     FKVGSYTVVDALVRYDLARVGMAGSNVALHVNNLFDREYVASCFNTYGCFWGAERQVV
                     ATATFRF"
     gene            12653..13450
                     /gene="fhuC"
                     /locus_tag="OO357_000710"
     CDS             12653..13450
                     /gene="fhuC"
                     /locus_tag="OO357_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706099.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe3+-hydroxamate ABC transporter ATP-binding
                     protein FhuC"
                     /protein_id="HCR5885761.1"
                     /translation="MQEYTNHSDTTFALRNISFRVPGRTLLHPLSLTFPAGKVTGLIG
                     HNGSGKSTLLKMLGRHQPPSEGEILLDAQPLESWSSKAFARKVAYLPQQLPPAEGMTV
                     RELVAIGRYPWHGALGRFGAADREKVEEAISLVGLKPLAHRLVDSLSGGERQRAWIAM
                     LVAQDSRCLLLDEPTSALDIAHQVDVLALVHRLSQERGLTVIAVLHDINMAARYCDYL
                     VALRSGEMIAQGTPAEIMRGETLEMIYGIPMGILPHPAGAAPVSFVY"
     gene            13450..14340
                     /gene="fhuD"
                     /locus_tag="OO357_000711"
     CDS             13450..14340
                     /gene="fhuD"
                     /locus_tag="OO357_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter
                     substrate-binding protein FhuD"
                     /protein_id="HCR5885762.1"
                     /translation="MSGLPLISRRRLLTAMALSPLLWQMNTAHAAAIDPNRIVALEWL
                     PVELLLALGIVPYGVADTINYRLWVSEPPLPDSVIDVGLRTEPNLELLTEMKPSFMVW
                     SAGYGPSSEMLARIAPGRGFNFSDGKHPLAMARKSLTEMADLLNLQSAAETHLAQYED
                     FIRSMKPRFVKRGARPLLLTTLIDPRHMLVFGPNSLFQEILDEYGIPNAWQGETNFWG
                     STAVSIDRLAAYKDVDVLCFDHDNSKDMDALMATPLWQAMPFVRTGRFQRVPAVWFYG
                     ATLSAMHFVRVLDNAIGGKA"
     gene            14337..16319
                     /gene="fhuB"
                     /locus_tag="OO357_000712"
     CDS             14337..16319
                     /gene="fhuB"
                     /locus_tag="OO357_000712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-hydroxamate ABC transporter permease
                     FhuB"
                     /protein_id="HCR5885763.1"
                     /translation="MSKRIALFPALLLALLVIVATALTWMNFSQALPRSQWAQAAWSP
                     DIDVIEQMIFHYSLLPRLAISLLVGAGLGLVGVLFQQVLRNPLAEPTTLGVATGAQLG
                     ITVTTLWAIPSAMASQFAALAGACVVGLIVFGVAWGKRLSPVTLIFAGLVVSLYCGAI
                     NQLLVIFHHDQLQSMFLWSTGTLTQTDWGGVERLWPQLLGGVMLTLLLLRPLTLMGLD
                     DGVARNLGLALSLARLAALSLAIVISALLVNAVGIIGFIGLFAPLLAKMLGARRLLPR
                     LMLASLIGALILWLSDQIILWLTRVWMEVSTGSVTALIGAPLLLWLLPRLRSISAPDM
                     KVNDRVATERQHVLAFALAGGVLLLMAVVVALSFGRDAHGWTWASGALLEDLMPWRWP
                     RIMAALFAGVMLAVAGCIIQRLTGNPMASPEVLGISSGAAFGVVLMLFLVPGNAFGWL
                     LPAGSLGAAVTLLIIMIAAGRGGFSPHRMLLAGMALSTAFTMLLMMLQASGDPRMAQV
                     LTWISGSTYNATDAQVWRTGIVMVILLAITPLCRRWLTILPLGGDTARAVGMALTPTR
                     IALLLLAACLTATATMTIGPLSFVGLMAPHIARMMGFRRTMPHIVISALVGGLLLVFA
                     DWCGRMVLFPFQIPAGLLSTFIGAPYFIYLLRKQSR"
     gene            complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="OO357_000713"
     CDS             complement(16354..17634)
                     /gene="hemL"
                     /locus_tag="OO357_000713"
                     /EC_number="5.4.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-1-semialdehyde 2,1-aminomutase"
                     /protein_id="HCR5885764.1"
                     /translation="MSKSENLYSAARELIPGGVNSPVRAFTGVGGTPLFIEKADGAYL
                     YDVDGKAYIDYVGSWGPMVLGHNHPAIRNAVIEAAERGLSFGAPTEMEVKMAQLVTEL
                     VPTMDMVRMVNSGTEATMSAIRLARGFTGRDKIIKFEGCYHGHADCLLVKAGSGALTL
                     GQPNSPGVPADFAKHTLTCTYNDLASVRAAFEQYPQEIACIIVEPVAGNMNCVPPLPE
                     FLPGLRALCDEFGALLIIDEVMTGFRVALAGAQDYFAVVPDLTCLGKIIGGGMPVGAF
                     GGRRDVMDALAPTGPVYQAGTLSGNPIAMAAGFACLNEVAQPGIHETLDELTTRLAEG
                     LLEAAEEAGIPLVVNHVGGMFGIFFTDAESVTCYQDVMACDVERFKRFFHMMLDEGVY
                     LAPSAFEAGFMSVAHSMEDINNTIDAARRVFAKL"
     gene            17859..19280
                     /gene="clcA"
                     /locus_tag="OO357_000714"
     CDS             17859..19280
                     /gene="clcA"
                     /locus_tag="OO357_000714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="H(+)/Cl(-) exchange transporter ClcA"
                     /protein_id="HCR5885765.1"
                     /translation="MKTDTPSLETPQAARLRRRQLIRQLLERDKTPLAILFMAAVVGT
                     LVGLAAVAFDKGVAWLQNQRMGALVHTADNYPLLLTVAFLCSAVLAMFGYFLVRKYAP
                     EAGGSGIPEIEGALEDQRPVRWWRVLPVKFFGGLGTLGGGMGLGREGPTVQIGGNISR
                     MVLDIFRLKGDEARHTLLATGAAAGLAAAFNAPLAGILFIIEEMRPQFRYTLISIKAV
                     FIGVIMSTIMYRIFNHEVALIDVGKLSDAPLNTLWLYLILGIIFGIFGPIFNKWVLGM
                     QDLLHRVHGGNITKWVLMGGAIGGLCGLLGFVAPATSGGGFNLIPIATAGNFSMGMLV
                     FIFVARVITTLLCFSSGAPGGIFAPMLALGTVLGTAFGMVAVELFPQYHLEAGTFAIA
                     GMGALLAASIRAPLTGIILVLEMTDNYQLILPMIITGLGATLLAQFTGGKPLYSAILA
                     RTLAKQEAEQLARSKAASASENT"
     gene            19304..19369
                     /locus_tag="OO357_000715"
     CDS             19304..19369
                     /locus_tag="OO357_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885766.1"
                     /translation="MAIIIGLEFARLPMSFGAKYE"
     gene            19362..19706
                     /gene="erpA"
                     /locus_tag="OO357_000716"
     CDS             19362..19706
                     /gene="erpA"
                     /locus_tag="OO357_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HCR5885767.1"
                     /translation="MSDDVALPLEFTDAAANKVKSLIADEDNPNLKLRVYITGGGCSG
                     FQYGFTFDDQVNEGDMTIEKQGVGLVVDPMSLQYLVGGSVDYTEGLEGSRFIVTNPNA
                     KSTCGCGSSFSI"
     gene            complement(19753..20376)
                     /locus_tag="OO357_000717"
     CDS             complement(19753..20376)
                     /locus_tag="OO357_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRIC cation channel family protein"
                     /protein_id="HCR5885768.1"
                     /translation="MLVYWLDIVGTAVFAIYGVLLAGKLRMDPFGVLVLGVVTAVGDG
                     TIRDMALDHGPVFWVKDPTDLVVAMVTSMLTIVLVRQPRRLPKWMLPVLDAVGLAVFV
                     GISVNKAFNAEAGPLIAVCMGVITGVGGGIIRDVLVREIPMILRTEIYATACIIGGIV
                     HATAYYTFSVPLETASMMGMVVTLLIRLAAIRWHLKLPTFALDENGR"
     gene            complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="OO357_000718"
     CDS             complement(20414..21214)
                     /gene="btuF"
                     /locus_tag="OO357_000718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter substrate-binding
                     protein BtuF"
                     /protein_id="HCR5885769.1"
                     /translation="MAKSLFRALVALSFLAPLWLNAAPRVITLSPANTELAFAAGITP
                     VGVSSYSDYPPQAQKIEQVSTWQGMNLERIVALKPDLVIAWRGGNAERQVDQLASLGI
                     KVMWVDATSIEQIANALRQLAPWSPQPDKAEQAAQSLLDQYVQLKAQYADKPKKRVFL
                     QFGINPPFTSGKESIQNQVLEVCGGENIFKDSRVPWPQVSREQVLARSPQAIVITGGP
                     DQIPKIKQYWGEQLKIPVIPLTSDWFERASPRIILAAQQLCNALSQVD"
     gene            complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="OO357_000719"
     CDS             complement(21207..21905)
                     /gene="mtnN"
                     /locus_tag="OO357_000719"
                     /EC_number="3.2.2.16"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HCR5885770.1"
                     /translation="MKIGIIGAMEEEVTLLRDKIENRQTISLGGCEIYTGQLNGTEVA
                     LLKSGIGKVAAALGATLLLEHCKPDVIINTGSAGGLAPTLKVGDIVVSDEARYHDADV
                     TAFGYEYGQLPGCPAGFKADDKLIAAAEACIAELNLNAVRGLIVSGDAFINGSVGLAK
                     IRHNFPQAIAVEMEATAIAHVCHNFNVPFVVVRAISDVADQQSHLSFDEFLAVAAKQS
                     SLMVESLVQKLAHG"
     gene            21989..23506
                     /gene="dgt"
                     /locus_tag="OO357_000720"
     CDS             21989..23506
                     /gene="dgt"
                     /locus_tag="OO357_000720"
                     /EC_number="3.1.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dGTPase"
                     /protein_id="HCR5885771.1"
                     /translation="MAQIDFRKKINWHRRYRSPQGVKTEHEILRIFESDRGRIINSPA
                     IRRLQQKTQVFPLERNAAVRTRLTHSMEVQQVGRYIAKEILSRLKELKLLEAYGLDEL
                     TGPFESIVEMSCLMHDIGNPPFGHFGEAAINDWFRQRLHPEDAESQPLTDDRCSVAAL
                     RLRDGEEPLNELRRKIRQDLCHFEGNAQGIRLVHTLMRMNLTWAQVGGILKYTRPAWW
                     RGETPETHHYLMKKPGYYLSEEAYIARLRKELNLALYSRFPLTWIMEAADDISYCVAD
                     LEDAVEKRIFTVEQLYHHLHEAWGQHEKGSLFSLVVENAWEKSRSNSLSRSTEDQFFM
                     YLRVNTLNKLVPYAAQRFIDNLPAIFAGTFNHALLEDASECSDLLKLYKNVAVKHVFS
                     HPDVEQLELQGYRVISGLLEIYRPLLSLSLSDFTELVEKERVKRFPIESRLFHKLSTR
                     HRLAYVEAVSKLPSDSPEFPLWEYYYRCRLLQDYISGMTDLYAWDEYRRLMAVEQ"
     gene            23636..25060
                     /gene="degP"
                     /locus_tag="OO357_000721"
     CDS             23636..25060
                     /gene="degP"
                     /locus_tag="OO357_000721"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegP"
                     /protein_id="HCR5885772.1"
                     /translation="MKKNTLALSALALSLGLALSPLSATAAETSSATTAQQMPSLAPM
                     LEKVMPSVVSINVEGSTTVNTPRMPRNFQQFFGDDSPFCQEGSPFQSSPFCQGGQGGN
                     GGGQQQKFMALGSGVIIDADKGYVVTNNHVVDNATVIKVQLSDGRKFDAKMVGKDPRS
                     DIALIQIQNPKKLTAIKMADSDALRVGDYTVAIGNPFGLGETVTSGIVSALGRSGLNA
                     ENYENFIQTDAAINRGNSGGALVNLNGELIGINTAILAPDGGNIGIGFAIPSNMVKNL
                     TSQMVEYGQVKRGELGIMGTELNSELAKAMKVDAQRGAFVSQVLPNSSAAKAGIKAGD
                     VITSLNGKPISSFAALRAQVGTMPVGSKLTLGLLRDGKQVNVNLELQQSSQNQVDSSS
                     IFNGIEGAEMSNKGKDQGVVVNNVKTGTPAAQIGLKKGDVIIGANQQAVKNIAELRKV
                     LDSKPSVLALNIQRGDSTIYLLMQ"
     gene            25215..26371
                     /gene="cdaR"
                     /locus_tag="OO357_000722"
                     /pseudo
     CDS             25215..26371
                     /gene="cdaR"
                     /locus_tag="OO357_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308193.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CdaR"
     gene            complement(26460..26846)
                     /locus_tag="OO357_000723"
     CDS             complement(26460..26846)
                     /locus_tag="OO357_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000272186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3461 family protein"
                     /protein_id="HCR5885773.1"
                     /translation="MYDNLKSLGITNPEEIDRYSLRQEANNDILKIYFQKDKGEFFAK
                     SVKFKYPRQRKTVVADGVGQGYKEVQEISPNLRYIIDELDQICQRDRSEVDLKRKILD
                     DLRHLESVVTNKISEIEADLEKLTRK"
     gene            complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="OO357_000724"
     CDS             complement(27160..27984)
                     /gene="dapD"
                     /locus_tag="OO357_000724"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HCR5885774.1"
                     /translation="MQQLQNIIETAFERRAEITPANADTVTREAVNQVIALLDSGALR
                     VAEKIDGQWVTHQWLKKAVLLSFRINDNQVIEGAESRYFDKVPMKFADYDEARFQKEG
                     FRVVPPAAVRQGAFIARNTVLMPSYVNIGAYVDEGTMVDTWATVGSCAQIGKNVHLSG
                     GVGIGGVLEPLQANPTIIEDNCFIGARSEVVEGVIVEEGSVISMGVYIGQSTRIYDRE
                     TGEIHYGRVPAGSVVVSGNLPSKDGKYSLYCAVIVKKVDAKTRGKVGINELLRTID"
     gene            complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="OO357_000725"
     CDS             complement(28015..30687)
                     /gene="glnD"
                     /locus_tag="OO357_000725"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     uridylyltransferase/uridylyl-removing protein GlnD"
                     /protein_id="HCR5885775.1"
                     /translation="MNTLPEQYANTALSTLPGQPQNPCAWPRDELTVCGIKAHIDTFQ
                     RWLGDAFDNGISAEQLIEARTEFIDQLLQRLWIEAGFSQIADLALVAVGGYGRGELHP
                     LSDIDLLILSRKKLPDDQAQKVGELLTLLWDVKLEVGHSVRTLEECMLEGLSDLTVAT
                     NLIESRLLIGDVALFLELQKHIFSEGFWPSDKFYAAKVEEQNQRHQRYHGTSYNLEPD
                     IKSSPGGLRDIHTLQWVARRHFGATSLDEMVGFGFLTSAERAELNECLHILWRIRFAL
                     HLVVSRYDNRLLFDRQLSVAQRLNYSGEGNEPVERMMKDYFRVTRRVSELNQMLLQLF
                     DEAILALPADEKPRPLDDEFQLRGTLIDLRDETLFMRQPEAILRMFYTMVRNSAITGI
                     YSTTLRQLRHARRHLQQPLCNIPQARKLFLSILRHPGAVRRGLLPMHRHSVLGAYMPQ
                     WSHIVGQMQFDLFHAYTVDEHTIRVMLKLESFASEETRQRHPLCVDVWPRLPSTELIF
                     IAALFHDIAKGRGGDHSILGAQDVVHFAELHGLNSRETQLVAWLVRQHLLMSVTAQRR
                     DIQDPEVIKQFAEEVQTENRLRYLVCLTVADICATNETLWNSWKQSLLRELYFATEKQ
                     LRRGMQNTPDMRERVRHHQLQALALLRMDNIDEEALHQIWSRCRANYFVRHSPNQLAW
                     HARHLLQHDLSKPLVLLSPQATRGGTEIFIWSPDRPYLFAAVCAELDRRNLSVHDAQI
                     FTTRDGMAMDTFIVLEPDGSPLSADRHEVIRFGLEQVLTQSSWQPPQPRRQPAKLRHF
                     TVETEVTFLPTHTDRKSFLELIALDQPGLLARVGKIFADLGISLHGARITTIGERVED
                     LFIIATADRRALNNELQQEVHQRLTEALNPNDKG"
     gene            complement(30749..31543)
                     /gene="map"
                     /locus_tag="OO357_000726"
     CDS             complement(30749..31543)
                     /gene="map"
                     /locus_tag="OO357_000726"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HCR5885776.1"
                     /translation="MAISIKTPEDIEKMRVAGRLAAEVLEMIEPYVKPGVSTGELDRI
                     CNDYIVNEQHAVSACLGYHGYPKSVCISINEVVCHGIPDDAKLLKDGDIVNIDVTVIK
                     DGFHGDTSKMFIVGKPTIMGERLCRITQESLYLALRMVKPGINLREIGAAIQKFVEAE
                     GFSVVREYCGHGIGRGFHEEPQVLHYDSRETNVVLKPGMTFTIEPMVNAGKKEIRTMK
                     DGWTVKTKDRSLSAQYEHTIVVTDNGCEILTLRKDDTIPAIISHDE"
     gene            31911..32636
                     /gene="rpsB"
                     /locus_tag="OO357_000727"
     CDS             31911..32636
                     /gene="rpsB"
                     /locus_tag="OO357_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HCR5885777.1"
                     /translation="MATVSMRDMLKAGVHFGHQTRYWNPKMKPFIFGARNKVHIINLE
                     KTVPMFNEALAELNKIVSRKGKILFVGTKRAASEAVKDAALSCDQFFVNHRWLGGMLT
                     NWKTVRQSIKRLKDLETQSQDGTFDKLTKKEALMRTRELEKLENSLGGIKDMGGLPDA
                     LFVIDADHEHIAIKEANNLGIPVFAIVDTNSDPDGVDFVIPGNDDAIRAVTLYLGAVA
                     ATVREGRSQDLASQAEESFVEAE"
     gene            32894..33745
                     /gene="tsf"
                     /locus_tag="OO357_000728"
     CDS             32894..33745
                     /gene="tsf"
                     /locus_tag="OO357_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HCR5885778.1"
                     /translation="MAEITASLVKELRERTGAGMMDCKKALTEANGDIELAIENMRKS
                     GAIKAAKKAGNVAADGVIKTKIDGNYGIILEVNCQTDFVAKDAGFQAFADKVLDAAVA
                     GKITDVEVLKAQFEEERVALVAKIGENINIRRVAALEGDVLGSYQHGARIGVLVAAKG
                     ADEELVKHIAMHVAASKPEFIKPEDVSAEVVEKEYQVQLDIAMQSGKPKEIAEKMVEG
                     RMKKFTGEVSLTGQPFVMEPSKTVGQLLKEHNAEVTGFIRFEVGEGIEKVETDFAAEV
                     AAMSKQS"
     gene            33892..34617
                     /gene="pyrH"
                     /locus_tag="OO357_000729"
     CDS             33892..34617
                     /gene="pyrH"
                     /locus_tag="OO357_000729"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000224576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HCR5885779.1"
                     /translation="MATNAKPVYKRILLKLSGEALQGTEGFGIDASILDRMAQEIKEL
                     VELGIQVGVVIGGGNLFRGAGLAKAGMNRVVGDHMGMLATVMNGLAMRDALHRAYVNA
                     RLMSAIPLNGVCDSYSWAEAISLLRNNRVVILSAGTGNPFFTTDSAACLRGIEIEADV
                     VLKATKVDGVFTADPAKDPTATMYEQLTYSEVLEKELKVMDLAAFTLARDHKLPIRVF
                     NMNKPGALRRVVMGEKEGTLITE"
     gene            34909..35466
                     /gene="frr"
                     /locus_tag="OO357_000730"
     CDS             34909..35466
                     /gene="frr"
                     /locus_tag="OO357_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000622421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HCR5885780.1"
                     /translation="MISDIRKDAEVRMDKCVEAFKTQISKIRTGRASPSLLDGIVVEY
                     YGTPTPLRQLASVTVEDSRTLKINVFDRSMSPAVEKAIMASDLGLNPNSAGSDIRVPL
                     PPLTEERRKDLTKIVRGEAEQARVAVRNVRRDANDKVKALLKDKEISEDDDRRSQDDV
                     QKLTDAAIKKIEAALADKEAELMQF"
     gene            35558..36754
                     /gene="ispC"
                     /locus_tag="OO357_000731"
     CDS             35558..36754
                     /gene="ispC"
                     /locus_tag="OO357_000731"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HCR5885781.1"
                     /translation="MKQLTILGSTGSIGCSTLDVVRHNPEHFRVVALVAGKNVTRMVE
                     QCLEFSPRYAVMDDEASAKLLKTMLQQQGSRTEVLSGQQAACDMAALEEVDQVMAAIV
                     GAAGLLPTLAAIRAGKTILLANKESLVTCGRLFMDAVKQSKAQLLPVDSEHNAIFQSL
                     PQPIQHNLGYADLEQNGVVSILLTGSGGPFRETPLRDLATMTPDQACRHPNWSMGRKI
                     SVDSATMMNKGLEYIEARWLFNASASQMEVLIHPQSVIHSMVRYQDGSVLAQLGEPDM
                     RTPIAHTMAWPNRVNSGVKPLDFCKLSALTFAAPDYERYPCLKLAMEAFEQGQAATTA
                     LNAANEITVAAFLAQQIRFTDIAALNLSVLEKMDMREPQCVDDVLSVDANASEVARKE
                     VMRLAS"
     gene            36943..37701
                     /gene="ispU"
                     /locus_tag="OO357_000732"
                     /gene_synonym="uppS"
     CDS             36943..37701
                     /gene="ispU"
                     /locus_tag="OO357_000732"
                     /gene_synonym="uppS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308203.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl-diphosphate-specific
                     ditrans,polycis-undecaprenyl-diphosphate synthase"
                     /protein_id="HCR5885782.1"
                     /translation="MLSATQPLSEKLPAHGCRHVAIIMDGNGRWAKKQGKIRAFGHKA
                     GAKSVRRAVSFAANNGIEALTLYAFSSENWNRPAQEVSALMELFVWALDSEVKSLHRH
                     NVRLRIIGDTSRFNSRLQERIRKSEALTAGNTGLTLNIAANYGGRWDIVQGVRQLAEK
                     VQQGNLQPDQIDEEMLNQHVCMHELAPVDLVIRTGGEHRISNFLLWQIAYAELYFTDV
                     LWPDFDEQDFEGALNAFANRERRFGGTEPGDETA"
     gene            37714..38571
                     /gene="cdsA"
                     /locus_tag="OO357_000733"
     CDS             37714..38571
                     /gene="cdsA"
                     /locus_tag="OO357_000733"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414717.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5885783.1"
                     /translation="MLKYRLISAFVLIPVVIAALFLLPPVGFAIVTLVVCMLAAWEWG
                     QLSGFTTRSQRVWLAVLCGLLLALMLFLLPEYHRNIHQPLVEISLWASLGWWIVALLL
                     VLFYPGSAAIWRNSKTLRLIFGVLTIVPFFWGMLALRAWHYDENHYSGAIWLLYVMIL
                     VWGADSGAYMFGKLFGKHKLAPKVSPGKTWQGFIGGLATAAVISWGYGMWANLDVAPV
                     TLLICSIVAALASVLGDLTESMFKREAGIKDSGHLIPGHGGILDRIDSLTAAVPVFAC
                     LLLLVFRTL"
     gene            38583..39935
                     /gene="rseP"
                     /locus_tag="OO357_000734"
     CDS             38583..39935
                     /gene="rseP"
                     /locus_tag="OO357_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706121.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma E protease regulator RseP"
                     /protein_id="HCR5885784.1"
                     /translation="MLSFLWDLASFIVALGVLITVHEFGHFWVARRCGVRVERFSIGF
                     GKALWRRTDKLGTEYVIALIPLGGYVKMLDERAEPVVPELRHHAFNNKSVGQRAAIIA
                     AGPVANFIFAIFAYWLVFIIGVPGVRPVVGEIAANSIAAEAQIAPGTELKAVDGIETP
                     DWDAVRLQLVDKIGDESTTITVAPFDSDQRRDVKLDLRHWAFEPDKEDPVSSLGIRPR
                     GPQIEPVLENVQPNSAASKAGLQAGDRIVKVDGQPLTQWVTFVMLVRDNPGKSLALEI
                     ERQGSPLSLTLIPESKPGNGKAIGFVGIEPKVIPLPDEYKVVRQYGPFNAIVEATDKT
                     WQLMKLTVSMLGKLITGDVKLNNLSGPISIAKGAGMTAELGVVYYLPFLALISVNLGI
                     INLFPLPVLDGGHLLFLAIEKIKGGPVSERVQDFCYRIGSILLVLLMGLALFNDFSRL
                     "
     gene            39965..42397
                     /gene="bamA"
                     /locus_tag="OO357_000735"
     CDS             39965..42397
                     /gene="bamA"
                     /locus_tag="OO357_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamA"
                     /protein_id="HCR5885785.1"
                     /translation="MAMKKLLIASLLFSSATVYGAEGFVVKDIHFEGLQRVAVGAALL
                     SMPVRTGDTVNDEDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGN
                     KSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPR
                     NRVDLKLVFQEGVSAEIQQINIVGNHAFTTDELISHFQLRDEVPWWNVVGDRKYQKQK
                     LAGDLETLRSYYLDRGYARFNIDSTQVSLTPDKKGIYVTVNITEGDQYKLSGVEVSGN
                     LAGHSAEIEQLTKIEPGELYNGTKVTKMEDDIKKLLGRYGYAYPRVQSMPEINDADKT
                     VKLRVNVDAGNRFYVRKIRFEGNDTSKDAVLRREMRQMEGAWLGSDLVDQGKERLNRL
                     GFFETVDTDTQRVPGSPDQVDVVYKVKERNTGSFNFGIGYGTESGVSFQAGVQQDNWL
                     GTGYAVGINGTKNDYQTYAELSVTNPYFTVDGVSLGGRLFYNDFQADDADLSDYTNKS
                     YGTDVTLGFPINEYNSLRAGLGYVHNSLSNMQPQVAMWRYLYSMGEHPSTSDQDNSFK
                     TDDFTFNYGWTYNKLDRGYFPTDGSRVNLTGKVTIPGSDNEYYKVTLDTATYVPIDDD
                     HKWVVLGRTRWGYGDGLGGKEMPFYENFYAGGSSTVRGFQSNTIGPKAVYFPHQASNY
                     DPDYDYECATQDGAKDLCKSDDAVGGNAMAVASLEFITPTPFISDKYANSVRTSFFWD
                     MGTVWDTNWDSSQYSGYPDYSDPSNIRMSAGIALQWMSPLGPLVFSYAQPFKKYDGDK
                     AEQFQFNIGKTW"
     gene            42519..43004
                     /gene="skp"
                     /locus_tag="OO357_000736"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
     CDS             42519..43004
                     /gene="skp"
                     /locus_tag="OO357_000736"
                     /gene_synonym="hlpA"
                     /gene_synonym="ompH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone Skp"
                     /protein_id="HCR5885786.1"
                     /translation="MKKWLLAAGLGLALATSAQAADKIAIVNMGSLFQQVAQKTGVSN
                     TLENEFKGRASELQRMETDLQAKMKKLQSMKAGSDRTKLEKDVMAQRQTFAQKAQAFE
                     QDRARRSNEERGKLVTRIQTAVKSVANSQDIDLVVDANAVAYNSSDVKDITADVLKQV
                     K"
     gene            43008..44033
                     /gene="lpxD"
                     /locus_tag="OO357_000737"
     CDS             43008..44033
                     /gene="lpxD"
                     /locus_tag="OO357_000737"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HCR5885787.1"
                     /translation="MPSIRLADLAQQLDAELHGDGDIVITGVASMQSAQTGHITFMVN
                     PKYREHLGLCQASAVVMTQDDLPFAKSAALVVKNPYLTYARMAQILDTTPQPAQNIAP
                     SAVIDATAKLGNNVSIGANAVIESGVELGDNVIIGAGCFVGKNSKIGAGSRLWANVTI
                     YHEIQIGQNCLIQSGTVVGADGFGYANDRGNWVKIPQIGRVIIGDRVEIGACTTIDRG
                     ALDDTVIGNGVIIDNQCQIAHNVVIGDNTAVAGGVIMAGSLKIGRYCMIGGASVINGH
                     MEICDKVTVTGMGMVMRPITEPGVYSSGIPLQPNKVWRKTAALVMNIDDMSKRLKSLE
                     RKVNQQD"
     gene            44138..44593
                     /gene="fabZ"
                     /locus_tag="OO357_000738"
     CDS             44138..44593
                     /gene="fabZ"
                     /locus_tag="OO357_000738"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000210741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HCR5885788.1"
                     /translation="MTTNTHTLQIEEILELLPHRFPFLLVDRVLDFEEGRFLRAVKNV
                     SVNEPFFQGHFPGKPIFPGVLILEAMAQATGILAFKSVGKLEPGELYYFAGIDEARFK
                     RPVVPGDQMIMEVTFEKTRRGLTRFKGVALVDGKVVCEATMMCARSREA"
     gene            44597..45385
                     /gene="lpxA"
                     /locus_tag="OO357_000739"
     CDS             44597..45385
                     /gene="lpxA"
                     /locus_tag="OO357_000739"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HCR5885789.1"
                     /translation="MIDKSAFVHPTAIVEEGASIGANAHIGPFCIVGPHVEIGEGTVL
                     KSHVVVNGHTKIGRDNEIYQFASIGEVNQDLKYAGEPTRVEIGDRNRIRESVTIHRGT
                     VQGGGLTKVGSDNLLMINAHIAHDCTVGNRCILANNATLAGHVSVDDFAIIGGMTAVH
                     QFCIIGAHVMVGGCSGVAQDVPPYVIAQGNHATPFGVNIEGLKRRGFSREAITAIRNA
                     YKLIYRSGKTLDEVKPEIAELAETYPEVKAFTDFFARSTRGLIR"
     gene            45385..46533
                     /gene="lpxB"
                     /locus_tag="OO357_000740"
     CDS             45385..46533
                     /gene="lpxB"
                     /locus_tag="OO357_000740"
                     /EC_number="2.4.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HCR5885790.1"
                     /translation="MTDQRPLTIALVAGETSGDILGAGLIRALKERVPNARFVGVAGP
                     RMQAEGCEAWYEMEELAVMGIVEVLGRLRRLLHIRADLTKRFGELKPDVFVGIDAPDF
                     NITLEGNLKKQGIKTIHYVSPSVWAWRQKRVFKIGRATDLVLAFLPFEKAFYDKYNVP
                     CRFIGHTMADAMPLDPDKNGARDVLGIPYDAHCLALLPGSRGAEVEMLSADFLKTAQL
                     LRQTYPDLEIVVPLVNAKRREQFERIKAEVAPDLSVHLLDGMGREAMVASDAALLASG
                     TAALECMLAKCPMVVGYRMKPFTFWLAKRLVKTDYVSLPNLLAGRELVKELLQEECEP
                     QKLAAALLPLLANGKTSHAMHDTFRELHQQIRCNADEQAAQAVLELAQ"
     gene            46530..47126
                     /gene="rnhB"
                     /locus_tag="OO357_000741"
     CDS             46530..47126
                     /gene="rnhB"
                     /locus_tag="OO357_000741"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HCR5885791.1"
                     /translation="MIEFVYPHTQLVAGVDEVGRGPLVGAVVTAAVILDPARPIAGLN
                     DSKKLSEKRRLALYEEIKEKALSWSLGRAEPHEIDELNILHATMLAMQRAVAGLHIAP
                     EYVLIDGNRCPKLPMPAMAVVKGDSRVPEISAASILAKVTRDAEMAALDIVFPQYGFA
                     QHKGYPTAFHLEKLAEYGATEHHRRSFGPVKRALGLAS"
     gene            47163..50645
                     /gene="dnaE"
                     /locus_tag="OO357_000742"
     CDS             47163..50645
                     /gene="dnaE"
                     /locus_tag="OO357_000742"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HCR5885792.1"
                     /translation="MSEPRFVHLRVHSDYSMIDGLAKTAPLVKKAAALGMPALAITDF
                     TNLCGLVKFYGAGHGAGIKPIVGADFNVQCDLLGDELTHLTVLAANNTGYQNLALLIS
                     KAYQRGYGAAGPIIDRDWLIELNEGLILLSGGRMGDVGRSLLRGNSALVDECVAFYEE
                     HFPDRYFLELIRTGRPDEESYLHAAVELAEARGLPVVATNDVRFIDSSDFDAHEIRVA
                     IHDGFTLDDPKRPRNYSPQQYMRSEEEMCELFADIPEALANTVEIAKRCNVTVRLGEY
                     FLPQFPTGDMSTEDYLVKRAKEGLEERLAFLFPDEEERLKRRPEYDERLETELQVINQ
                     MGFPGYFLIVMEFIQWSKDNGVPVGPGRGSGAGSLVAYALKITDLDPLEFDLLFERFL
                     NPERVSMPDFDVDFCMEKRDQVIEHVADMYGRDAVSQIITFGTMAAKAVIRDVGRVLG
                     HPYGFVDRISKLIPPDPGMTLAKAFEAEPQLPEIYEADEEVKALIDMARKLEGVTRNA
                     GKHAGGVVIAPTKITDFAPLYCDEEGKHPVTQFDKSDVEYAGLVKFDFLGLRTLTIIN
                     WALEMINKRRAKNGEPPLDIAAIPLDDKKSFDMLQRSETTAVFQLESRGMKDLIKRLQ
                     PDCFEDMIALVALFRPGPLQSGMVDNFIDRKHGREEISYPDVQWQHESLKSVLEPTYG
                     IILYQEQVMQIAQVLSGYTLGGADMLRRAMGKKKPEEMAKQRSVFAEGAEKNGINAEL
                     AMKIFDLVEKFAGYGFNKSHSAAYALVSYQTLWLKAHYPAEFMAAVMTADMDNTEKVV
                     GLVDECWRMGLKILPPDINSGLYHFHVNDDGEIVYGIGAIKGVGEGPIEAIIEARNKG
                     GYFRELFDLCARTDTKKLNRRVLEKLIMSGAFDRLGPHRAALMNSLGDALKAADQHAK
                     AEAIGQADMFGVLAEEPEQIEQSYASCQPWPEQVVLDGERETLGLYLTGHPINQYLKE
                     IERYVGGVRLKDMHPTERGKVITAAGLVVAARVMVTKRGNRIGICTLDDRSGRLEVML
                     FTDALDKYQQLLEKDRILIVSGQVSFDDFSGGLKMTAREVMDIDEAREKYARGLAISL
                     TDRQIDDQLLNRLRQSLEPHRSGTIPVHLYYQRADARARLRFGATWRVSPSDRLLNDL
                     RGLIGSEQVELEFD"
     gene            50658..51617
                     /gene="accA"
                     /locus_tag="OO357_000743"
     CDS             50658..51617
                     /gene="accA"
                     /locus_tag="OO357_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HCR5885793.1"
                     /translation="MSLNFLDFEQPIAELEAKIDSLTAVSRQDEKLDINIDEEVHRLR
                     EKSVELTRKIFADLGAWQIAQLARHPQRPYTLDYVRLAFDEFDELAGDRAYADDKAIV
                     GGIARLDGRPVMIIGHQKGRETKEKIRRNFGMPAPEGYRKALRLMQMAERFKMPIITF
                     IDTPGAYPGVGAEERGQSEAIARNLREMSRLGVPVVCTVIGEGGSGGALAIGVGDKVN
                     MLQYSTYSVISPEGCASILWKSADKAPLAAEAMGIIAPRLKELKLIDSIIPEPLGGAH
                     RNPEAMAASLKAQLLADLADLDVLSTEDLKNRRYQRLMSYGYA"
     gene            51716..53857
                     /gene="ldcC"
                     /locus_tag="OO357_000744"
     CDS             51716..53857
                     /gene="ldcC"
                     /locus_tag="OO357_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine decarboxylase LdcC"
                     /protein_id="HCR5885794.1"
                     /translation="MNIIAIMGPHGVFYKDEPIKELESALVAQGFQIIWPQNSVDLLK
                     FIEHNPRICGVIFDWDEYSLDLCSDINQLNEYLPLYAFINTHSTMDVSVQDMRMALWF
                     FEYALGQAEDIAIRMRQYTNEYLDNITPPFTKALFTYVKERKYTFCTPGHMGGTAYQK
                     SPVGCLFYDFFGGNTLKADVSISVTELGSLLDHTGPHLEAEEYIARTFGAEQSYIVTN
                     GISTSNKIVGMYAAPSGSTLLIDRNCHKSLAHLLMMNDVVPVWLKPTRNALGILGGIP
                     RREFTRDSIEEKVAATTQAQWPVHAVITNSTYDGLLYNTDWIKQTLDVPSIHFDSAWV
                     PYTHFHPIYQGKSGMSGERVAGKVFFETQSTHKMLAALSQASLIHIKGEYDEEAFNEA
                     FMMHTTTSPSYPIVASVKTAAAMLRGNPGKRLINRSVERALHFRKEVQRLREESDGWF
                     FDIWQPPQVDEAECRPVAPGEQWHGFNDADADHMFLDPVKVTILTPGMDEQGNMSEEG
                     IPAALVAKFLDERGIVVEKTGPYNLLFLFSIGIDKTKAMGLLRGLTEFKRSYDLNLRI
                     KNMLPDLYAEDPDFYRNMRIQDLAQGIHKLIRKHDLSGLMLRAFDTLPEMIMTPHQAW
                     QRQIKGEVETIALEQLVGRVSANMILPYPPGVPLLMPGEMLTKESRTVLDFLLMLCSV
                     GQHYPGFETDIHGAKQDEDGVYRVRVLKMAG"
     gene            53914..54303
                     /locus_tag="OO357_000745"
     CDS             53914..54303
                     /locus_tag="OO357_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5885795.1"
                     /translation="MLGLKQVHHIAIIATDYAVSKAFYCDILGFTLQSEVYREARDSW
                     KGDLALNGQYVIELFSFPFPPERPSRPEACGLRHLAFSVDDIDAAVAHLESHNVKCEA
                     IRVDPYTQKRFTFFNDPDGLPLELYEQ"
     gene            54368..55666
                     /gene="tilS"
                     /locus_tag="OO357_000746"
     CDS             54368..55666
                     /gene="tilS"
                     /locus_tag="OO357_000746"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HCR5885796.1"
                     /translation="MTLTLNRQLLTSRQILVAFSGGLDSTVLLHQLVQWRTENPGVTL
                     RAIHVHHGLSANADAWVTHCENVCQQWQVPLVVERVQLAQEGLGIEAQARQARYQAFA
                     RTLLPGEVLVTAQHLDDQCETFLLALKRGSGPAGLSAMAEVSEFAGTRLIRPLLARTR
                     GELEQWALAHGLRWIEDESNQDDSYDRNFLRLRVVPLLQQRWPHFAEATARSATLCAE
                     QESLLDELLADDLAHCQTSQGTLQIAPMLAMSDARRAAIIRRWLAGQNAPMPSRDALV
                     RIWQEVALAREDASPCLRLGAFEIRRYQSQLWWIKSVTGQSETIVLWQTWLQPLELPA
                     GLGTVQLTAGGDIRPPRADEAVSVRFKAPGLLHIVGRNGGRKLKKIWQELGVPPWLRD
                     TTPLLFYGETLIAAAGVFVTQEGVAEGENGVSFVWQKTLS"
     gene            complement(55715..55975)
                     /gene="rof"
                     /locus_tag="OO357_000747"
     CDS             complement(55715..55975)
                     /gene="rof"
                     /locus_tag="OO357_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rho-binding antiterminator"
                     /protein_id="HCR5885797.1"
                     /translation="MSMNDTYQPINCDDYDNLELACQHHLMLTLELKDGEKLQAKASD
                     LVSRKNVEYLVVEAAGETRELRLDKITSFSHPEIGTVVVSES"
     gene            complement(55962..56162)
                     /locus_tag="OO357_000748"
     CDS             complement(55962..56162)
                     /locus_tag="OO357_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeP family protein"
                     /protein_id="HCR5885798.1"
                     /translation="MEKYCELIRKRYAEIASGDLGYVPDALGCVLKVLNEMAADDALS
                     EAVREKAAYAAANLLVSDYVNE"
     gene            56328..56873
                     /locus_tag="OO357_000749"
     CDS             56328..56873
                     /locus_tag="OO357_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeQ family protein"
                     /protein_id="HCR5885799.1"
                     /translation="MALKATIYKATVNVADLDRNQFLDASLTLARHPSETQERMMLRL
                     LAWLKYADERLQFTRGLCADDEPEAWLRNDHLGIDLWIELGLPDERRIKKACTQAAEV
                     ALFTYNSRAAQIWWQQNQSKCVQFANLSVWYLDDEQLAKVSAFADRTMTLQATIQDGV
                     IWLSDDKNNLEVNLTAWQQPS"
     gene            56870..57292
                     /gene="arfB"
                     /locus_tag="OO357_000750"
     CDS             56870..57292
                     /gene="arfB"
                     /locus_tag="OO357_000750"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5885800.1"
                     /translation="MIVISRHVAIPDGELEITAIRAQGAGGQHVNKTSTAIHLRFDIR
                     ASSLPEYYKERLLAASHHLISSDGVIVIKAQEYRSQELNREAALARLVAVIKDLTKEQ
                     KARRPTRPTRASKERRLASKAQKSSVKAMRGKVRSGRE"
     gene            57306..58016
                     /gene="nlpE"
                     /locus_tag="OO357_000751"
                     /gene_synonym="cutF"
     CDS             57306..58016
                     /gene="nlpE"
                     /locus_tag="OO357_000751"
                     /gene_synonym="cutF"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706138.1"
                     /note="NlpE, an outer membrane lipoprotein, interacts
                     directly with CpxA, the sensor histidine kinase of the Cpx
                     system for response to envelope stress; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response activation lipoprotein
                     NlpE"
                     /protein_id="HCR5885801.1"
                     /translation="MVKKAIVTAMAVISLFTLMGCNNRAEVDTLSPAQAAELKPMPQS
                     WRGVLPCADCEGIEISLFLEKDGTWVMNERYLGAREEPSSFASYGTWARTADKLVLTD
                     SKGEKSYYRAKGDALEMLDREGNPIESQFNYTLEPAQSSLPMTPMTLRGMYFYMADAA
                     TFTDCATGKRFMVANNAELERSYLAARGHSEKPVLLSVEGHFTLEANPDTGAPTKVLA
                     PDTAGKFYPNKDCSSLGQ"
     gene            complement(58171..58995)
                     /locus_tag="OO357_000752"
     CDS             complement(58171..58995)
                     /locus_tag="OO357_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706139.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaeF family permuted papain-like enzyme"
                     /protein_id="HCR5885802.1"
                     /translation="MDKPKAYCRLLLPSFLLLSACTVDISQPDPSATAVDAEAKTWAV
                     KFQHQSSFTEQSIKEITAPDLKPGDLLFSSSLGVTSFGIRVFSTSSVSHVAIFLGDNN
                     VAEATGAGVQIVSLKKAMKHSDKLFVLRVPDLTPQQATDITAFANKIKDSGYNYRGIV
                     EFIPFMVTRQMCSLNPFSEDFRQQCVSDLAKAQLSSVCEGDKKSWFCSEFVTDAFAKS
                     GHPLTLAQSGWISPADLMHMRIGDVSAFKPETQLQYVGHLKPGIYIKAGRFVGLTQ"
     gene            complement(59048..60766)
                     /gene="proS"
                     /locus_tag="OO357_000753"
     CDS             complement(59048..60766)
                     /gene="proS"
                     /locus_tag="OO357_000753"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HCR5885803.1"
                     /translation="MRTSQYLLSTLKETPADAEVISHQLMLRAGMIRKLASGLYTWLP
                     TGVRVLKKVENIVREEMNNAGAIEVSMPVVQPADLWQESGRWEQYGPELLRFVDRGER
                     PFVLGPTHEEVITDLIRNELSSYKQLPLNFYQIQTKFRDEVRPRFGVMRSREFLMKDA
                     YSFHTSQESLQETYDAMYAAYSKIFSRMGLDFRAVQADTGSIGGSASHEFQVLAQSGE
                     DDVVFSDTSDYAANIELAEAIAPKEPRAAATQEMTLVDTPNAKTIAELVEQFNLPIEK
                     TVKTLLVKAVEGSSFPLVALLVRGDHELNEVKAEKLPQVASPLTFATEEEIRAVVKAG
                     PGSLGPVNMPIPVVIDRTVAAMSDFAAGANIDGKHYFGINWDRDVATPEVADIRNVVA
                     GDPSPDGQGTLLIKRGIEVGHIFQLGTKYSEALKASVQGEDGRNQILTMGCYGIGVTR
                     VVAAAIEQNYDERGIVWPDAIAPFQVAILPMNMHKSFRVQELAEKLYSELRAQGIEVL
                     LDDRKERPGVMFADMELIGIPHTIVLGDRNLDNDDIEYKYRRNGEKQLIKTGDIVEYL
                     VKQIKG"
     gene            complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="OO357_000754"
     CDS             complement(60877..61584)
                     /gene="tsaA"
                     /locus_tag="OO357_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase
                     TrmO"
                     /protein_id="HCR5885804.1"
                     /translation="MSSFQFEQIGVIRSPYKEKFAVPRQPGLVKSANGELHLIASYNQ
                     ADAVRGLEAFSHLWILFVFHQTMEGGWRPTVRPPRLGGNARMGVFATRSTFRPNPIGM
                     SLVELKEVVCHKGSVILKLGSLDLVDGTPVVDIKPYLPFAESLPDASASYAQSAPAAE
                     MAVSFTAEVEKQLLTLEKRYPQLTLFIREVLAQDPRPAYRKGEETGKTYAVWLHDFNV
                     RWRVTDAGFEVFALEPR"
     gene            complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="OO357_000755"
     CDS             complement(61581..61985)
                     /gene="rcsF"
                     /locus_tag="OO357_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rcs stress response system protein RcsF"
                     /protein_id="HCR5885805.1"
                     /translation="MRALPICLVALMLSGCSMLSRSPVEPVQSTAPQPKAEPAKPKAP
                     RATPVRIYTNAEELVGKPFRDLGEVSGDSCQASNQDSPPSIPTARKQMQINASKMKAN
                     AVLLHSCEVTSGTPGCYRQAVCIGSALNITAK"
     gene            complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="OO357_000756"
     CDS             complement(62103..62918)
                     /gene="metQ"
                     /locus_tag="OO357_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter substrate-binding
                     lipoprotein MetQ"
                     /protein_id="HCR5885806.1"
                     /translation="MAFKFKTFAAVGALIGSLALVGCGQDEKDPNHIKVGVIVGAEQQ
                     VAEVAQKVAKDKYGLDVELVTFNDYVLPNEALSKGDIDANAFQHKPYLDQQLKDRGYK
                     LVAVGNTFVYPIAGYSKKIKSLDELQDGSQVAVPNDPTNLGRSLLLLQKVGLIKLKDG
                     VGLLPTVLDVVENPKNLKIVELEAPQLPRSLDDAQIALAVINTTYASQIGLTPAKDGI
                     FVEDKDSPYVNLIVTREDNKDAENVKKFVQAYQSDEVYEAANKVFNGGAVKGW"
     gene            complement(62958..63611)
                     /gene="metI"
                     /locus_tag="OO357_000757"
     CDS             complement(62958..63611)
                     /gene="metI"
                     /locus_tag="OO357_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter permease MetI"
                     /protein_id="HCR5885807.1"
                     /translation="MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPG
                     QIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAP
                     FIARMVENALLEIPTGLIEASRAMGATPMQIVCKVLLPEALPGLVNAATITLITLVGY
                     SAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK"
     gene            complement(63604..64635)
                     /gene="metN"
                     /locus_tag="OO357_000758"
     CDS             complement(63604..64635)
                     /gene="metN"
                     /locus_tag="OO357_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine ABC transporter ATP-binding protein
                     MetN"
                     /protein_id="HCR5885808.1"
                     /translation="MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGK
                     STLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGN
                     VALPLELDNTPKDEIKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPK
                     VLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGE
                     LIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQTEPFTDCVPMLRLEFTGQ
                     SVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHH
                     VKVEVLGYV"
     gene            64823..65398
                     /gene="gmhB"
                     /locus_tag="OO357_000759"
     CDS             64823..65398
                     /gene="gmhB"
                     /locus_tag="OO357_000759"
                     /EC_number="3.1.3.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose 1,7-bisphosphate
                     7-phosphatase"
                     /protein_id="HCR5885809.1"
                     /translation="MAKSVPAIFLDRDGTINVDHGYVHEIDNFEFIDGVIDAMRELKK
                     MGFALVVVTNQSGIARGKFTEAQFETLTEWMDWSLADRDVDLDGIYYCPHHPQGSVEE
                     FRQVCDCRKPHPGMLLSARDYLHIDMAASYMVGDKLEDMQAAVAANVGTKVLVRTGKP
                     ITPEAENAADWVLNSLADLPQAIKKQQKPAQ"
     gene            65762..>66032
                     /locus_tag="OO357_000760"
     rRNA            65762..>66032
                     /locus_tag="OO357_000760"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT        15626 a        16347 c        18197 g        15862 t
ORIGIN      
        1 tgggattgat atttatctgc gggaatttat attatgtttc tgcaaaaatg tttaaactaa
       61 aatttttatt aaggaaagag aggatgtatg attttcagaa aatatttata ttcgtaatat
      121 aaataaatta ccatttgctt aatttgtcaa atgctcttgt atgtaagact tcacgcaacg
      181 tctccccatc aggaaacgcc agctccggtg caatctcaaa cagcggccac agcataaatc
      241 cacgattctt catatcgtag tgcggaacgg tcaggcgttc ggtatttatc acttcattac
      301 caaacagcat gatatcgaga tccagcgtgc gtggtcccca gcgttcagct ttacgaacac
      361 gaccctgttg cagttctatg cgctgtgtat ggttgagtaa ctcttccggg gcgggcgaag
      421 tttcaagcgc cacggcggcg ttgaggtaat cgggttgatc ttgcggcccc agcggtgggg
      481 tgcggtaaaa cgaagaaacg gcaagaatgc ggctttcagg gatatcgcct aatgctttca
      541 gggcagcatt gacctgctcc agcggagagg ccagattgct gcctatggca atatacgcca
      601 ctgtcatgtg gtaccctcac gacgcggtgc gcgtttgcgt ggacgacgag tacgacgacg
      661 cggtgacggt tcttcatcca gctcgttaag catccctttt tgatctggtg gtgcggaaac
      721 ctggaactca ccccaccatt tcaccagacg ctgcagttca gcattacgtt caacttcagc
      781 tcgcaaggcc aacaggtcat aagccgcacg gaacttagga tgctccagca gtttccatgc
      841 gcgtttaccc tgacgacggg acatacgcaa ctgcaactgc cagatatcgc gggttaacgt
      901 cgtcagacgt ttcgggattg ccagtgaacg gcaggcttcg tccagcacgt cgttcatcgc
      961 cagcgcgaaa gcgtcgtgat aggtcaggcc gctttcctgg gcgatcttct gtgccgtctc
     1021 cagcagtggg taccagaaca tggcggcaaa caagaacgcc gggttcacgc gcatatcgtt
     1081 atggatacgc gtatcggtat tcttcagcac ctgttcaatg atccgctcca tcgggctgtc
     1141 gccattttcc gtgaagtagc gggtaatggt cgggaacagc ggctggaaca gatgatattc
     1201 acacaacagc ttataggttt cgtaaccgta gcccgcttgt agcagtttaa gcgattcttc
     1261 aaacaggcgt gccggtggga tatcgttcag cagggtagcg aggcgaggga tcggttctgc
     1321 ggtttccggg ctgatgcgca tacccaattt ggcggcaaaa cgtaccgcgc gcagcatacg
     1381 taccggatct tcacggtagc gcgtttccgg gttaccaatc agacggataa cgccgtcctt
     1441 cagatccttc ataccaccaa cgtaatcacg gacggtaaaa tccgctacgc tgtaatacag
     1501 gctgttgata gtgaaatcgc ggcgctgggc gtcttcttcg atggagccga aaatgttgtc
     1561 gcgcagcaac atgccgtttt gcccgcgttg ggaggtcgtg cggtcgctga cgttaccttc
     1621 gtggtgtcca cggaaggtcg caacttcgat aatctccggg ccaaacatta catgagccag
     1681 acggaaacgg cgacccacca ggcggcagtt acggaacagt ttgcgcacct gctcaggcgt
     1741 ggcgttagtg gttacgtcaa aatctttcgg ctttttgcca agtaacaggt cgcgcacgcc
     1801 gccgccaacc agccaggctt cgtatcccgc tttgttgagc ctgtacatta ccttcagggc
     1861 attttcactg atatctttgc gggaaatagc atgctgctca cgcgggatca ccgtcacctg
     1921 tggacgggcg actgcctgtt cagcctcgct ttcctcgcgg cttagcacct tgcggcaaaa
     1981 attagcgact cgggtaaaaa tagtacacct cggtagtgtc aaacatcatt caggacaaaa
     2041 aaatagcggc taatcatagc tcagcatgac gcatttgaga atgttgaatt tacaattgcc
     2101 gactcgggca cggcggtcag catccagttt ttgacggctg actgaaggat ttgctcgacg
     2161 ctgaaatcct gccagtgtgc ttctgcctgc tgccccagaa attgaagtgc cgcgattagt
     2221 accgggcgtg gatcgccttt cggcaacgca ggcgcatgat tctgcttgga aagtttagcg
     2281 ccttgtggat taagcgccag cggcagatga atgtaatctg gcactttcca gccaaaaagc
     2341 tggtacagcg ggatttgcct tactgttggt tcaatcagat cagccccacg cactatttct
     2401 gtaacgccct ggaaatgatc atcaaccaca acagccaggt tgtaggcgaa caacccatca
     2461 cggcgatgaa tgataaaatc ttcccgtgcc agtttttcgt cggcgtgaat aatgcctcgc
     2521 agcaggtcag taaattgcgt gaccggatgc tgctggcgga tacgcactgc ggcgttgtct
     2581 ggtccatgat gcaacacccg gcaatgaccg tcgtaaatac cgccaatgct ttgaatacgc
     2641 gcacgcgtac aggtgcagta ataacttagt ccttgttcat gtaaccaggc gagtgcttca
     2701 cgataggcgt cgtgacgttg cgattgccag agaacatcgc cgtcccagtg cagaccgtaa
     2761 tgttccagct ggcgcaggat agtttctgcg gcaccgggaa cttcacgagg cgggtcgata
     2821 tcttctatgc gtaccagcca gcgaccttgc cgggcgcgag cctgcaaata gctgccgagc
     2881 gcggcgatca gagagccaaa atgaagctcg ccggaaggag agggggcgaa gcggccaata
     2941 tactgtgtgt ctgtcatctc tttgaacaaa aaataaggcg ggagcatttc ccgcctgtgg
     3001 taaacgtgat ggaacggctg taattagcca gccatctgtt tttcgcgaat ttcagccagc
     3061 gttttgcagt cgatgcacag atcggctgtc gggcgcgctt ccagacggcg aataccaatt
     3121 tcaacaccgc aggattcgca gtagccgaaa tcttcgtctt ccactttttt cagcgtcttc
     3181 tcgatctttt tgatcagctt acgctcgcga tcgcggttac gcagttcgag gctgaactct
     3241 tcttcctggg ctgcacggtc taccgggtcc gggaagttgg ctgcttcatc ctgcatatgt
     3301 gtaacggtgc gatcgacttc atccctgagt tgattacgcc atgcttccag aatacgacgg
     3361 aagtgcgcca gctgggcttc attcatatac tcttcgcccg gcttctcctg atatggttcc
     3421 accccagcga tggcgagaat actcagggac gatgttttac ggttttgccc ttcttgcatg
     3481 ttgcttctcc ttaacacgca ctatcgatcc ccatgttcgg gggaaaaatg aggccgctat
     3541 aaatagcaga tgcttttccg gatagcaatt atctaaacgt aacacttgac aactgtgtga
     3601 ggaaaagcgt atttgcgcac gcgaccagaa tgtaaattaa ccagttactt actttactac
     3661 aatgtaaccg gcagtgattt tttaagagcc atgccttcag cagaaatttc cgctttgtaa
     3721 gccagaattt ctacccccct ctgttgagct tctgacaata gttgcgcgta tttctcatcg
     3781 atgtggcgcg cgggtgaaaa ccgtgtaatg gctgaatgca gcacggcgaa aaaaataacc
     3841 gcacgctggc cttcagccgc tacgctcatc aactcccgaa ggtgtttctg acctcgttca
     3901 gtgaccgcat cgggaaaata tccctgttcg ttctccgcta acgtaaccga tttcacttca
     3961 atatagcagt ctggacgcga atccgcctgc aacataaagt caatacggct gcgttcggag
     4021 ccgtatttta cttcgctttt cagcgagcta tagcctgaca gttctgaaat tgattcatta
     4081 aggatagcct ctttcgtcaa cctgttagcc caaagcgtat tgacgcaaat aaatgcgccg
     4141 ctctggcttt gagttaattc ccaggtgtgt gggtatttcc gtttggtgtt gtctgaagtc
     4201 gaataccaga cggtatcgcc aggcgttgca caaccggtca tcgcacccgt attcgggcag
     4261 tgtagcgtta attcgcgacc atcgggtgtg atcacatcgg ctaaaaaacg tttgtaacgc
     4321 tgaattagcg tcgcgcgctg tagaggggga gaaaattcca ttgcgacaat ccttgttatt
     4381 gcgttagcgc ccagcgtttt agcggcgtgt agcgtgtgcg tccacgggca aacgaggagg
     4441 cgtaaagggt gaactccgtc accgcataag accagttaaa acccggcggc gggaatgtca
     4501 ccgcctcgct ggcgtcgcgc aataaggtaa tatgtggatg aaacggacga ttgctttgaa
     4561 aacaaccgct gcgggcagcc tgtgaacgga gcatattcgc cagctggatt aagccgcgtg
     4621 gaggctgacg catccctaac cacaccacac gcgaacgcag ccattgtccg gcgtcatcaa
     4681 gcgtgagtgt gaaaccaggt tgacgaatcc gtccggctaa aagagaaagc gccttctctt
     4741 tctctgcgct cacttcgcct aaaaatgcca gagtcaggtg caaattatcg gcggcgaccg
     4801 gacgtcccgc ctcaggtggg aagtgtgtgg cgcgccaatg gataatctgt tcgcggattt
     4861 ctgcaggtaa gtcgatagca aagaacagac gttgcggttc agacatgtga ggcactcggt
     4921 tatgaattac cgcgatgcta caatgtggcg cgaagaatgt taaccctctg gagcgttttg
     4981 tgtcgtcgtt gcccgttgct gccgtcttac ctgaattact taccgctctc gattgtgcgc
     5041 cgcaggtatt attaagtgcg ccgaccgggg ccgggaaatc aacctggctg ccgctgcaac
     5101 tgctggcgca tcccggcatt aacgggaaaa ttatcctgct ggagccgcgt cgtctggcgg
     5161 cgcgtaacgt cgcgcaacgg ctggcggagc tgcttaacga aaagccaggc gataccgttg
     5221 gctaccggat gcgtgcgcaa aactgcgtcg ggctgaatac ccgcctggaa gtggttaccg
     5281 aaggcgtgct gacgcggatg atccagcgtg acccggaact gagcggtgtt ggattggtga
     5341 tccttgatga gtttcatgag cgcagtttgc aggcggattt ggcgttggcg ctgttactcg
     5401 atgtgcaaca aggtctgcgt gatgacctta aactgctgat tatgtcggct acgctggaca
     5461 atgaccgctt gcagcaaatg ctgccggaag cgcccgtcgt catctcagaa gggcgctcgt
     5521 ttccggttga acgccgttat ttaccgctgc ccgcgcatca gcgttttgac gaagccgttg
     5581 cggtagccac cgccgaaatg gtgcgtcagg aaagtggatc attactgtta tttttacctg
     5641 gcgtcggaga aattcagcgc gtgcaggaac aactggcttc gcgcatcggc agtgatgtgg
     5701 tgctctgccc gctgtatggc gtgttgtcac tgaacgatca gcgaaaagcg atcctcccgg
     5761 caccgcaagg gatgcgcaaa gtggtgctgg cgaccaatat tgctgaaacc agtttaacca
     5821 tcgaaggtat tcgtctggtg gtggattgtg cccaggagcg tgtggcgcgc tttgatccgc
     5881 gcacggggct tacgcggttg attactcaac gcgttagtca ggcatccatg acgcagcgtg
     5941 ccgggcgcgc cgggcgtctg gagccgggta tcagcctgca tttaatcgcc aaagaacaag
     6001 cagaacgcgc cgcggcgcaa agtgagccgg agatcttaca aagcgatctt tccggtttgc
     6061 tgatggaatt actgcaatgg ggatgcagcg atccggcgca gatgagctgg ctggatcaac
     6121 cgccaacggt gaatctactg gccgcgaaac gcctgttaca aatgctgggg gcgctggagg
     6181 gtgaacggct tagtgcgcaa gggcaaaaaa tggcagcgct gggtaacgat ccgcgtttag
     6241 cggcaatgct ggttaacgca aagagcgatg acgaagctgc taccgcggca aaaattgccg
     6301 ccattctcga agagccgcca cggatgggca atagtgacct gggcgtggcg ttttcgcgca
     6361 atcaaccagc ctggcagcaa cgtagtcagc aactgttaaa acgcttaaac gtacgcggcg
     6421 gtgaggcaga cagttcgctt atcgcgccgc tacttgccgg ggcgtttgcc gatcgcattg
     6481 ctcatcgccg tgggcaagat ggacgctatc aactggcaaa cagcatggga gcgatgctcg
     6541 atgccgacga cgcgctaagc cgccacgaat ggttgatcgc accgttatta ttgcagggca
     6601 gcgcctcgcc ggatgcgcgg attttactgg cgctaccggt cgatattgat gaattagtac
     6661 aacgctgtcc gcagctggtg cagcaatctg acaccgtgga atgggatgac gcgcaaggta
     6721 cgctgaaagc ctggcgtcgg ctacaaatcg gtcagttgac ggtgaaagtg cagccgctgg
     6781 cgaaaccgtc agaagacgag ttgcatcagg cgatgcttaa tggcatccgt gataaaggtt
     6841 taagcgtgct caactggacg gcggaagcgg aacagctacg cttgcgtttg ttatgcgccg
     6901 caaagtggtt gccggaatat gactggccag cggttgatga tgaaagtttg ttggcgacgc
     6961 tggaaacgtg gctgctgcca catatgaccg gcgtacattc actacgcggc ctgaaatcac
     7021 tcgacattta tcaggcacta cgcggattac ttgattgggg aatgcagcaa cgtctggata
     7081 gtgaattgcc tgcgcattac actgtgccga cgggaagccg gatcgccatt cgttatcatg
     7141 aagataaccc gcccgcgctg gcggtgagaa tgcaggagat gtttggtgag gccactaatc
     7201 cgacgatcgc ccaggggcgc gtgccgctgg tgctgaagtt gctttcccct gcccaaaggc
     7261 cgctgcaaat cacgcgtgat ttgggggcct tctggaaagg ggcgtatcgt gaggtgcaaa
     7321 aggagatgaa agggcgttat cccaaacacg tctggccgga cgacccggca aacacggcac
     7381 cgacgcgacg gacgaaaaag tattcgtaag gttggttttc tccctctccc tgtgggagag
     7441 ggccggggtg agggcatcag cgcgcacgtt caccctcacc ctaaccctct ccctcaaggg
     7501 agaggggacc gatcgagcgc aaattttgag agatatcttc ttctgtcttg taacagaaga
     7561 acagaaaatc gggcttttgc gcctgaatat tgcggagaaa aagcatggcc gggaatgacc
     7621 gcgagccaat tggacgcaaa gggaaaccga cgcgtccggt caaacaaaag gtaagccgtc
     7681 gtcgttacga agatgacgat gattacgacg attatgatga ctatgaggat gaagaaccga
     7741 tgccgcgcaa aggtaagggc aaaggcaaag ggcgtaagcc tcgtggcaaa cgcggctggc
     7801 tatggctact gctaaaactg gctatcgttt ttgccgtgct gatcgccatt tacggcgttt
     7861 atctcgatca aaaaattcgt agccgtattg atggcaaggt ctggcaactg cccgcggcag
     7921 tttatggccg aatggtcaac cttgaaccag atatgaccat cagcaagaac gaaatggtga
     7981 agctgctgga ggcaacacag tatcgtcagg tgtcgaaaat gacccgtcct ggcgaattta
     8041 ccgtgcaggc caacagcatt gagatgatcc gccgtccgtt tgatttcccg gacagtaaag
     8101 aagggcaggt gcgtgcgcgt ctgacctttg atggtgatca tctggcgacc atcgtcaata
     8161 tggagaacaa ccgtcagttc ggtttcttcc gtcttgatcc gcgtctgatc accatgatct
     8221 cctcgccaaa cggtgagcag cgtttgtttg tgccgcgcag tggtttccct gatttgctgg
     8281 tggatacttt gctggcgaca gaagaccgtc atttttacga gcatgatgga atcagtctct
     8341 actccattgg gcgtgcggtg ctggcaaacc tgaccgcagg tcgcacggtg cagggtgcga
     8401 gtacgctgac gcaacagctg gtgaaaaacc tgttcctctc cagcgagcgt tcttactggc
     8461 gtaaagcgaa cgaagcttac atggcgctga tcatggacgc gcgttacagc aaagaccgta
     8521 ttcttgagct gtatatgaac gaggtgtatc tcggtcagag cggcgacaac gaaatccgcg
     8581 gcttcccgct ggcaagcttg tattactttg gtcgcccggt agaagagcta agcctcgacc
     8641 agcaggcgct gttagtcggt atggtgaaag gggcgtccat ctacaacccg tggcgtaacc
     8701 cgaaactggc gctggagcga cgtaatctgg tgctgcgtct gctgcaacag caacagatta
     8761 ttgatcaaga actctatgac atgttgagtg cccgtccgct gggggttcag ccgcgtggtg
     8821 gggtgatctc tcctcagcca gcctttatgc agctggtgcg tcaggagttg caggcaaaac
     8881 tgggcgataa ggtaaaagat ctctccggcg tgaagatctt cactaccttt gactcggtgg
     8941 cccaggacgc ggcagaaaaa gccgccgtgg aaggcattcc ggcactgaag aaacagcgta
     9001 agttgagcga tcttgaaact gcgattgtgg tcgtcgaccg ctttagtggt gaagttcgtg
     9061 cgatggtcgg aggttctgag ccgcagtttg cgggctacaa ccgtgcgatg caggcgcgtc
     9121 gttcgattgg ttcccttgca aaaccagcga cttatctgac ggctttaagc cagccgaaaa
     9181 tctatcgcct gaacacgtgg attgccgatg cgccaatcgc gctgcgtcag ccgaatggcc
     9241 aggtctggtc accgcagaac gatgaccgtc gttatagcga aagcggcaga gtgatgctgg
     9301 tggatgcgtt gacacgttcg atgaacgtgc cgacggtaaa tctggggatg gcgctggggc
     9361 tgcctgcggt tacggagacc tggatcaaac tgggcgtacc gaaagatcag ttgcatccgg
     9421 ttccggcaat gctgctgggg gcgttgaact taacgccaat cgaagtggcg caggcattcc
     9481 agaccatcgc cagcggtggt aaccgtgcac cgctttctgc actgcgttcg gtaatcgcgg
     9541 aagatggtaa ggtgctgtat cagagcttcc cgcaggcaga acgcgctgtt ccggcgcagg
     9601 cggcgtatct gacactatgg accatgcagc aggtggtaca acgtggtacg ggtcgtcagc
     9661 ttggggcgaa atacccgaac ctgcatctgg cagggaaaac agggactacc aacaataacg
     9721 tagatacctg gtttgcaggc attgacggca gcacggtgac catcacctgg gtaggccgtg
     9781 ataacaacca gccgaccaaa ctgtatggag ccagcggggc aatgtcgatt tatcagcgtt
     9841 atctggctaa ccagacgcca acgccgctga atcttgttcc gccagaagat attgcagata
     9901 tgggcgtgga ctacgacggc aactttgttt gcagcggtgg catgcgtgtc ttgccggtct
     9961 ggaccagcga tccgcaatcg ctgtgccagc agagcgagat gcagcagcag ccgtcaggca
    10021 atccgtttga tcagtcttct cagccgcagc aacagccgca acagcaacct gctcagcaag
    10081 agcagaaaga cagcgacggt gtagccggtt ggatcaagga tatgtttggt agtaattaac
    10141 atctaagcgt gaaataccgg atggcgagtt gccatccggt aaaataacat cccatctaag
    10201 atattaaccc tttcttttca tctggttgtt tattaaccct tcaggaacgc tcagattgcg
    10261 taccgcttgc gaacccgcca gcgtttcgaa tattatctta tctttataat aatcattctc
    10321 gtttacgtta ttattcactt tacatcagag atataccaat ggcgcgttcc aaaactgctc
    10381 agccaaaaca ctcactgcgt aaaatcgcag ttgtagtagc cacagcggtt agcggcatgt
    10441 ctgtttatgc acaggcagcg gttgaaccga aagaagacac tatcaccgtt accgctgcac
    10501 ctgcgccgca agaaagcgca tgggggcctg ctgcaactat tgcggcgcgg cagtcagcta
    10561 ccggcactaa aaccgatacg ccgattcaaa aagtgccaca gtctatttct gttgtgaccg
    10621 ccgaagagat ggcgctgcat cagccgaagt cggtaaaaga agcgcttagc tacacgccgg
    10681 gtgtctctgt tggtacgcgt ggcgcatcca acacctatga ccacctgatc attcgcggtt
    10741 ttgcggcaga aggccaaagc caaaataact atctgaatgg cctgaagttg cagggcaact
    10801 tctataacga tgcggtcatt gacccgtata tgctggaacg cgctgaaatt atgcgtggcc
    10861 cggtttccgt gctttacggt aaaagcagtc ctggcggtct gttgaatatg gtcagcaagc
    10921 gtccgaccac cgaaccgctg aaagaagttc agtttaaagc cggtactgac agcctgttcc
    10981 agactggttt tgactttagc gatgcgttgg atgatgacgg tgtttactct tatcgcctga
    11041 ccggtcttgc gcgttctgcc aatgcccagc agaaagggtc agaagagcag cgttatgcta
    11101 ttgcaccggc gttcacctgg cgtccggatg ataaaaccaa tttcaccttc ctttcttact
    11161 tccagaacga gccggaaacc ggttattacg gctggttgcc gaaagaggga accgttgagc
    11221 cgctgccgaa cggtaagcgt ctgccgacag actttaatga aggggcgaag aacaacacct
    11281 attctcgtaa tgagaagatg gtcggctaca gcttcgatca cgaatttaac gacaccttta
    11341 ctgtgcgtca gaacctgcgc tttgctgaaa acaaaacctc gcaaaacagc gtttatggtt
    11401 acggcgtctg ctccgatccg gcgaatgctt acagcaaaca gtgtgcggca ttagcgccag
    11461 cggataaagg ccattatctg gcacgtaaat acgtcgttga tgatgagaag ctgcaaaact
    11521 tctccgttga tacccagttg cagagcaagt ttgccactgg cgatatcgac cacaccctgc
    11581 tgaccggtgt cgactttatg cgtatgcgta atgacatcaa cgcctggttt ggttacgacg
    11641 actctgtgcc actgttcaat ctgtacaatc cggtgaatcc cgatttcgac ttcaatgcca
    11701 aagatccggc aaactccggc ccttaccgca ttctgaataa acagaaacaa acgggcgttt
    11761 atgttcagga tcaggcgcag tgggataaag tgctggtcac cctaggcggt cgttatgact
    11821 gggcagatca agaatctctt aaccgcgttg ctgggacgac cgataaacgt gatgacaaac
    11881 agtttacctg gcgtggtggt gttaactacc tgtttgataa tggtgtaaca ccttacttca
    11941 gctatagcga atcgtttgaa ccttcttcgc aagttgggaa ggatggtaat attttcgcac
    12001 cgtctaaagg taagcagtat gaagtcggcg tgaaatatgt accggaagat cgtccgattg
    12061 tagttactgg tgccgtgtat aatctcacta aaaccaacaa cctgatggcg gaccctgagg
    12121 gttccttctt ctcggttgaa ggtggcgaga tccgcgcacg tggcgtagaa atcgaagcga
    12181 aagcggcgct gtcggcgagt gttaacgtag tcggttctta tacttacacc gatgcggaat
    12241 acaccaccga tactacctat aaaggcaata cgcctgcaca ggtgccaaaa cacatggctt
    12301 cgctgtgggc tgactatacc ttctttgacg gtccgctttc aggtctgacg ctgggcaccg
    12361 gtggtcgtta tactggctcc agctatggtg atccggctaa ctcctttaaa gtgggaagtt
    12421 atacggtcgt ggatgcgtta gtgcgttatg atctggcgcg agtcggcatg gcgggctcca
    12481 acgtggcgct gcatgttaac aacctgttcg atcgtgaata cgtcgccagc tgctttaaca
    12541 cttatggctg cttctggggc gcagaacgtc aggtcgttgc aaccgcaacc tttcgtttct
    12601 aatttctctt ttggggcacg gatttccgtg cccatttcac aagttggctg ttatgcagga
    12661 atacacgaat cattccgata ccacttttgc actgcgtaat atctcctttc gtgtgcccgg
    12721 gcgcacgctt ttgcatccgc tgtcgttaac ctttcctgcc gggaaagtga ccggtctgat
    12781 tggtcacaac ggttctggta aatccactct gctcaaaatg cttggtcgtc atcagcctcc
    12841 gtcagaaggg gagattcttc ttgatgccca gccgctggaa agctggagca gcaaagcgtt
    12901 tgcccgcaaa gtggcttatt tgccgcagca gcttcctccg gcagaaggga tgaccgtgcg
    12961 tgaactggtg gcgattggtc gttacccgtg gcatggcgcg ctggggcgct ttggtgcggc
    13021 agatcgcgaa aaggtcgagg aagctatctc gctggttggc ttaaaaccgc tggcgcatcg
    13081 gctggtcgat agtctctctg gcggcgaacg tcagcgggcg tggatcgcca tgctggtggc
    13141 gcaggatagc cgttgtctgt tgctcgacga accgacttca gcgctggata tcgcccacca
    13201 ggttgatgtg ctggcgctgg tgcaccgttt aagtcaggaa cgtggcctga cggtcattgc
    13261 cgtgttgcac gatatcaata tggcggcacg ctactgtgat tatctggtcg ccctgcgcag
    13321 tggtgaaatg attgctcagg gaacgcctgc ggaaattatg cgcggcgaaa ccctcgaaat
    13381 gatttatggc atcccgatgg gtattttgcc gcatccggcg ggtgctgcac ctgtgagttt
    13441 tgtttattga tgagcggctt acctcttatt tcgcgccgtc gactgttaac ggcgatggcg
    13501 ctttctccgt tgttatggca gatgaatacc gcccacgcgg cggctattga ccccaatcgt
    13561 attgtggcgc tggagtggtt gccggtggaa ttactgctgg cgctcggcat cgtgccttac
    13621 ggcgtggcgg ataccatcaa ctatcgcctg tgggtcagcg aaccaccatt gccggactca
    13681 gtgattgacg tcggtttgcg tacagaacct aaccttgaac tgctgaccga aatgaaaccg
    13741 tcgtttatgg tctggtcggc aggatatggc ccttcatcag aaatgctggc acgtattgcg
    13801 ccgggtcgcg gatttaactt cagtgacggc aaacatccgt tggcgatggc gcgtaaatcg
    13861 ctgacggaaa tggcagattt acttaacctg caaagcgcag cggaaacgca tttagcgcaa
    13921 tacgaagact ttatccgcag catgaaaccc cgctttgtga agcgtggtgc acgcccgtta
    13981 ctgctgacga cgcttatcga tccgcgccat atgctggtct tcggtccaaa cagcttgttc
    14041 caggaaattc tcgatgagta cggcatccca aatgcctggc aaggggaaac caacttctgg
    14101 ggcagtaccg ccgtcagtat cgatcgtctg gcagcgtata aagacgttga tgtgctctgt
    14161 tttgatcacg acaacagcaa agacatggat gcgctaatgg caaccccgct gtggcaggcc
    14221 atgccgtttg tccgtacagg gcgctttcag cgcgtacccg cagtctggtt ttatggtgca
    14281 acgctttcgg caatgcactt tgtgcgtgtt ctggataacg ccatcggagg taaagcgtga
    14341 gtaaacgaat tgcgcttttc ccggcgttat tgctggcgct gttagtgatt gtcgctacgg
    14401 cgctcacctg gatgaacttc tcacaggcgc tgccgcgcag tcagtgggcg caggccgcct
    14461 ggtcgccgga tattgacgtc atcgagcaga tgatttttca ctacagcttg ttgccgcgtc
    14521 tggcgatttc gctgctggtg ggcgcgggcc tggggctggt gggcgtgctg tttcagcaag
    14581 tactgcgtaa cccgctggcg gaaccgacga cgttgggcgt tgcaacaggc gcgcaactgg
    14641 ggattaccgt caccacgctc tgggcgatcc ctagcgcgat ggcgagccag tttgctgcgc
    14701 tggcaggggc ttgcgttgtt ggcttaattg tctttggcgt cgcgtggggg aaacggctgt
    14761 cgccggtaac gctgattttc gcggggttgg tagtgagcct ttattgcggc gcaatcaatc
    14821 agttactggt tatcttccat catgaccagt tgcaaagcat gtttctgtgg agcactggaa
    14881 cgctgacgca aaccgactgg ggcggtgttg agcgtttatg gccgcagttg ctgggcggtg
    14941 tgatgctgac gttgctgcta cttcgtccgt taaccctgat ggggcttgat gatggcgtgg
    15001 cgcgcaatct cgggctggcc ttgtcgcttg cgcgtctggc ggcgctgtcg ctggcgattg
    15061 tcatcagtgc gctgctggtg aacgctgtgg ggattatcgg ctttatcggg ttgttcgcgc
    15121 cgctgctggc aaaaatgctg ggggcgcggc gtctgctgcc acgactgatg ctggcgtcgt
    15181 tgattggtgc gctgatcctc tggctttccg atcaaatcat cctctggctg actcgcgtgt
    15241 ggatggaagt gtccaccggt tcggtcactg cgttgatcgg tgcgccgctg ctactgtggc
    15301 tgctgccgcg tttacgcagc attagcgcgc cggatatgaa ggttaacgat cgtgtcgcga
    15361 ctgaacgcca acatgtgctg gcgtttgccc tcgcgggcgg cgtgctgttg ttgatggctg
    15421 tggtggtggc gctgtcgttt ggtcgtgatg cgcacggctg gacgtgggcg agcggggcgt
    15481 tgctcgagga tttaatgccc tggcgctggc cgcgaattat ggcggcgctg tttgcgggcg
    15541 tcatgctggc ggtagcgggc tgtattattc agcgactgac cggcaacccg atggcaagcc
    15601 cggaagtgct ggggattagc tccggcgcgg cgtttggtgt ggtgttaatg ctgtttctgg
    15661 taccgggtaa tgcctttggc tggttgttac ccgcaggcag tctcggggcg gcggtgacgc
    15721 tgttgatcat tatgatcgcc gccggacgcg gtggattttc cccacaccgc atgttactgg
    15781 cggggatggc gttaagtacc gcgttcacca tgcttttgat gatgttgcag gcaagtggtg
    15841 acccgcgaat ggcgcaagtg ctgacctgga tttccggttc gacctacaac gcgaccgatg
    15901 ctcaggtctg gcgcaccgga attgtgatgg tgattttgct ggcgattact ccgctgtgcc
    15961 gccgctggct gaccatttta ccgctgggtg gtgataccgc gcgggcggta gggatggcgc
    16021 tgacgccgac gcgaattgcg ctgctgctgt tagcggcttg cctgacggcg accgcgacga
    16081 tgactattgg accgttgagt tttgttggtt taatggcacc gcatattgcg cggatgatgg
    16141 gctttcgacg gacgatgcca cacatcgtaa tttcggcgct ggtgggtggt ttactgctgg
    16201 tgttcgctga ctggtgtggg cggatggtgc tgtttccatt ccagatcccg gcggggctgc
    16261 tgtcgacctt tatcggtgcg ccatatttta tctatttgct gagaaagcag agccgttaat
    16321 agaatgcctc gcccggtggg cgaggcaaaa ttatcacaac ttcgcgaaca cccgacgtgc
    16381 agcatcgatg gtgttattga tatcttccat gctgtgcgcc acagacataa agcctgcttc
    16441 aaacgctgac ggtgccaggt aaacaccttc atccagcatc atatggaaga aacgcttaaa
    16501 acgctcgacg tcacaggcca tcacatcctg atagcacgtc acggactcgg cgtcggtaaa
    16561 gaagataccg aacataccac cgacgtggtt aacgaccagc ggaattcccg cttcttctgc
    16621 cgcttccagc agaccttctg ccagacgtgt tgtcagctca tccagcgttt cgtgaatgcc
    16681 cggctgcgcg acttcattca gacaggcgaa acccgctgcc atcgcaatcg ggttaccgga
    16741 aagcgtaccc gcctgataga ccggacctgt cggggccagc gcatccatta catcacgacg
    16801 accaccgaat gcgcctaccg gcattccacc gccgatgatt tttccgaggc aggttaaatc
    16861 tggcactacg gcgaaataat cctgtgcgcc agctagcgct acgcggaaac cggtcatcac
    16921 ttcatcgatg atcagcaacg cgccaaattc gtcgcacagc gcacgcagac ctggcaggaa
    16981 ctctggcagc ggcggaacac agttcatatt gcctgccacc ggctcgacga taatacaggc
    17041 aatctcttgc gggtattgct caaacgcggc gcgtacagag gccagatcgt tataagtaca
    17101 ggttaaggta tgtttggcga aatctgccgg aacgcccggc gagtttggct ggcctaacgt
    17161 gagtgcgcca gaaccggctt tcaccagcag gcagtcagcg tgaccgtggt aacaaccttc
    17221 aaatttaatg attttgtcgc gaccggtaaa accacgggcc aggcggatgg cgctcatcgt
    17281 cgcctcggtg ccggagttca ccatgcgcac catatccatg gtcgggacca gttcggtcac
    17341 cagttgcgcc attttcactt ccatttcggt cggtgcacca aagcttaaac cacgctcggc
    17401 ggcttcaatc acggcattgc ggatcgccgg atggttatgg cccagcacca tcggccccca
    17461 ggaaccgaca taatcgatat aggctttgcc atcaacatcg tacagataag cgccgtccgc
    17521 tttttcgata aacagtggag tgccgcccac gccagtaaag gcgcgaacag gggagttcac
    17581 accgccaggg atcagctcgc gcgctgcgct gtaaagattt tcagacttac tcatggaggg
    17641 ttcctgattc gtagaaaaag tgaatggctg ctattccatg ttattcataa caagttaaat
    17701 actcgtcaaa catcaggctg cttgtactgg tgcaatcctg atttcttaga gtataaaagt
    17761 tttgtgcatt tgaaacatta cgctttgcaa aggattttca tggaacgtgc gagtaaaatg
    17821 ccgtcatctt atttgtatga ccaataagtg atcattggat gaaaactgat actccctctt
    17881 tagaaacacc gcaggccgcg cgcctgcgac gcagacaact gattcgccaa cttcttgagc
    17941 gcgataaaac cccgttagcc attttgttta tggcggcagt cgtcggcacg cttgtcgggc
    18001 tggctgcggt tgcttttgac aaaggtgtcg cctggttgca gaaccaacgt atgggggcgc
    18061 tggtacatac tgctgataat tatccgctac tgttaaccgt cgcttttctc tgttcggcgg
    18121 tgctggcgat gttcggctac tttttggtgc gcaaatacgc gcccgaagca gggggatcgg
    18181 ggatcccgga aattgaaggg gcgctggaag atcaacgtcc tgttcgctgg tggcgtgtat
    18241 tgccggtgaa attctttggc gggctgggga cactcggcgg aggcatgggg ctagggcgcg
    18301 aaggaccaac cgtgcagatc ggcggcaata tcagtcgcat ggtgcttgat attttccgcc
    18361 tgaaaggtga tgaagctcgc catacgctgc tggcaaccgg tgctgctgcg gggctggctg
    18421 cggcctttaa cgcgccgctg gcgggtattt tgtttattat cgaagagatg cgtccgcagt
    18481 ttcgctatac gttaatttcg attaaagcgg tatttattgg cgtcattatg tcgaccatta
    18541 tgtaccggat ttttaatcat gaagttgcgt tgattgacgt cggtaaactt tctgatgcgc
    18601 cgcttaatac gctgtggctt tatctgatcc tcggtattat ttttggcatt ttcggcccta
    18661 tttttaataa atgggtgctg gggatgcagg atttgctgca ccgtgtgcac ggcggcaata
    18721 ttaccaaatg ggtactgatg gggggggcga ttggcggtct gtgtggattg ctggggtttg
    18781 tggcaccagc aacgtcgggc ggcggtttta acctgattcc tatcgctacc gcggggaatt
    18841 tcagcatggg aatgctggtg tttatcttcg tcgctcgggt cattaccacc ttactctgct
    18901 tctcttccgg cgcgccgggc ggtatttttg ccccgatgct ggcgctgggt actgtgctgg
    18961 gaaccgcttt cggaatggtt gccgttgagc tgtttccgca atatcacctg gaggcgggga
    19021 cgtttgctat tgccggaatg ggggcattac tggcggcatc tattcgcgcg ccgttaacgg
    19081 ggatcattct ggttctggag atgaccgata actaccagct cattttgcca atgattatta
    19141 ccggtcttgg cgcaacacta ttagcgcaat ttaccggcgg gaaacctcta tactcggcga
    19201 ttcttgcgcg cacgctggca aaacaggaag ctgagcaact ggcgcgaagc aaggccgcat
    19261 cagccagcga gaatacttga acgaaatacc agggtattag ataatggcga ttattattgg
    19321 gttagaattt gcccgattgc cgatgtcgtt tggagcaaaa tatgagtgat gacgtagcac
    19381 tgccgctgga gtttaccgac gcagcagcca acaaagttaa aagcctgatc gctgacgaag
    19441 ataacccgaa tctgaaatta cgcgtgtata tcaccggtgg cggttgcagc ggcttccagt
    19501 atggtttcac ctttgatgat caggtgaacg aaggcgatat gaccatcgaa aaacagggcg
    19561 ttggcctggt ggttgatccg atgagcctgc aatatctggt cggcggttcc gttgactaca
    19621 ccgaaggact ggaaggttct cgtttcatcg tgaccaaccc gaacgcgaaa agcacctgcg
    19681 gttgcggttc ttcctttagc atctaatctg ttgttcatgt gccggataaa ctatccggca
    19741 catcttctct cttcaacgcc cattctcatc cagcgcaaac gtcggtagct taagatgcca
    19801 acgaatagcc gccagccgaa tcaatagcgt cacgaccatg cccatcatac tggctgtttc
    19861 cagtggtacg gaaaatgtgt aataagccgt agcgtggaca ataccgccga taatacaggc
    19921 agttgcgtag atttctgtac gtaaaatcat ggggatttcg cggaccagta catcacgaat
    19981 gatcccgccg ccaacgccag taatgacgcc catacaaacc gcgattaacg gaccggcttc
    20041 cgcattaaag gctttattca cgctaatgcc gacaaacacc gccagaccaa cggcgtccag
    20101 caccggcaac atccattttg gtaagcgtct tggctggcgc accagcacga tggtcagcat
    20161 gctggtgacc atggcaacga ccagatcggt gggatctttc acccaaaata ccgggccgtg
    20221 atccagcgcc atgtcgcgaa ttgttccgtc gcctactgcg gtaaccacgc ccagtaccag
    20281 aacaccaaaa gggtccatac gcaattttcc ggctaacaaa acgccgtaga tggcaaatac
    20341 cgctgtgccg actatatcca gccaatagac gagcattgtt caatccccac tgagcacctg
    20401 aaaaggtcag gcactaatct acctgtgaaa gcgcattaca gagctgttgt gctgcgagga
    20461 taatacgtgg gcttgcacgt tcaaaccagt cactcgtgag aggaataacg ggaattttga
    20521 gctgttcacc ccagtattgt ttgattttag gaatttggtc cggtccgcct gtaatgacaa
    20581 tagcctgtgg cgagcgtgct aacacctgtt cgcggctaac ttgcggccag ggaacccggc
    20641 tgtctttaaa gatgttttct ccgccacaaa cttcgagtac ctggttctga atcgactctt
    20701 ttccactggt aaatggcgga ttaatgccga attgcagaaa aacacgtttt ttaggtttat
    20761 cagcatattg cgctttcaat tgcacgtact gatccagcag ggattgcgcg gcttgttcgg
    20821 ccttgtctgg ttgcggactc cagggggcca gttgacgtaa cgcattggca atttgttcaa
    20881 tgcttgtcgc atcgacccac atcactttta ttcccagcga agccagctgg tcaacctgcc
    20941 gctcggcatt acctccacgc caggcaatca ccagatcggg tttcagcgcg acaatgcgtt
    21001 ccagattcat cccctgccag gtggaaacct gctcaatctt ttgcgcttgt ggaggatagt
    21061 cggaatagct gctgacccca accggcgtga tcccggcggc aaaggcaagt tcagtgttgg
    21121 cgggagaaag cgtgatgacg cgcggcgcgg cgttgagcca cagtggcgcg agaaaagaca
    21181 gggcgaccag cgccctgaac agtgatttag ccatgtgcca gtttctgcac cagtgactca
    21241 accatcaggc tggactgttt agcggcaaca gccaggaact catcgaagct aagatgagac
    21301 tgttgatcgg ccacgtcgga gatggcgcgt acgacaacaa acgggacgtt gaaattgtgg
    21361 cagacatggg cgattgccgt cgcttccatc tctacagcaa tggcctgtgg gaagttgtgg
    21421 cggattttcg ccagaccaac agaaccgttg atgaaagcgt cgccgctaac aatcaggcca
    21481 cgtacagcgt taagattcag ttcggcaatg caggcctcag cggcagcgat cagtttatcg
    21541 tcagctttaa agcctgccgg acagcctggt aactgaccgt attcataacc aaatgccgtg
    21601 acatccgcgt cgtgataacg cgcttcgtcc gagacaacga tatcgcccac tttcagcgtt
    21661 ggtgccaggc caccggcaga accggtgtta ataatcacat ctggcttgca gtgttccaac
    21721 agcagagtgg cacccagcgc cgcagcgact ttaccgatgc ccgatttcag aagcgcaacc
    21781 tcggttccat tcagttggcc ggtatagatt tcgcaaccgc cgagactgat agtttgacgg
    21841 ttttcgattt tgtcacgcag cagcgtaact tcttcttcca ttgcaccaat gatgccgatt
    21901 ttcatagatt tactcgcgat aagcccgatt tgaaggcata gtttaccatg cgcttacggg
    21961 gaagcgtatt tctcacgcgg gagaggacat ggcacagatt gatttccgaa aaaaaataaa
    22021 ctggcatcgt cgttaccgtt caccgcaggg cgttaaaacc gaacatgaga tcctgcggat
    22081 cttcgagagc gatcgcgggc gtatcatcaa ctctccggca attcgtcgtc tgcaacaaaa
    22141 gacccaggtt tttccactgg agcgcaatgc cgccgtgcgc acgcgtctta cccactcgat
    22201 ggaagtccag caggtgggac gctacatcgc caaagaaatt ttaagccgtc tgaaagagct
    22261 taaattgctg gaagcatacg gcctggatga actgaccggc ccctttgaaa gcattgttga
    22321 gatgtcatgc ctgatgcacg atatcggcaa tccgccgttt ggtcattttg gcgaagcagc
    22381 gataaatgac tggtttcgcc aacgtttgca cccggaagat gccgaaagcc agcctctgac
    22441 tgacgatcgc tgcagcgtgg cggcactacg tttacgggac ggggaagaac cgcttaacga
    22501 gctgcggcgc aagattcgtc aggacttatg tcattttgag gggaatgcac aaggcattcg
    22561 tctggtgcat acattgatgc ggatgaatct cacttgggca caggttggcg gtattttaaa
    22621 atatacccgt ccggcgtggt ggcgtggcga aacgcctgag acacatcact atttaatgaa
    22681 aaagccgggt tattatcttt ctgaagaagc ctatattgcc cgcttgcgta aagaacttaa
    22741 tttggcgctt tacagtcgtt ttccattaac gtggattatg gaagctgccg acgacatctc
    22801 ctattgtgtg gcagaccttg aagatgcggt agagaaaaga atatttaccg ttgagcagct
    22861 ttatcatcat ttgcacgaag cgtggggcca gcatgagaaa ggttcgctct tttcgctggt
    22921 ggttgaaaat gcctgggaaa aatcacgctc aaatagttta agccgcagta cggaagatca
    22981 gttttttatg tatttacggg taaacaccct aaataaactg gtaccctacg cggcacaacg
    23041 atttattgat aatctgcctg cgattttcgc cggaacgttt aatcatgcat tattggaaga
    23101 tgccagcgaa tgcagcgatc ttcttaagct atataaaaat gtcgctgtaa aacatgtgtt
    23161 tagccatccg gatgtcgagc agcttgaatt gcagggctat cgggtcatta gcggattatt
    23221 agagatttat cgtcctttat taagcctgtc gttatcagac tttactgaac tggtagaaaa
    23281 agaacgggtg aaacgtttcc ctattgagtc gcgcttattc cacaaactct cgacgcgcca
    23341 tcggctggcc tatgtcgagg ctgtcagtaa attaccgtca gattctcctg agtttccgct
    23401 atgggaatat tattaccgtt gccgcctgct gcaggattat atcagcggta tgaccgacct
    23461 ctatgcgtgg gatgaatacc gacgtctgat ggccgtagaa caataaccag tcttttgtaa
    23521 agacgaacaa taaattttta ccttttgcag aaactttagt tcggaacttc gggctataaa
    23581 acgaatctga agaacacagc aattttgcgt tatctgttaa tcgagactga aatacatgaa
    23641 aaaaaacaca ttagcactga gtgcactggc tctgagttta ggtttggcgt tatctccgct
    23701 ctctgcaacg gcggctgaga cttcttcagc aacgacagcc cagcagatgc caagccttgc
    23761 accgatgctc gaaaaggtga tgccttcagt ggtcagcatt aacgtagaag gtagcacaac
    23821 cgttaatacg ccgcgtatgc cgcgtaattt ccagcagttc ttcggtgatg attctccgtt
    23881 ctgccaggaa ggttctccgt tccagagctc tccgttctgc caggggggcc agggcggtaa
    23941 tggcggcggc cagcaacaga aattcatggc gctgggttcc ggcgtcatca ttgatgccga
    24001 taaaggctat gtcgtcacca acaaccacgt tgttgataac gcgacggtga ttaaagtcca
    24061 gctgagcgat ggtcgtaagt tcgacgcgaa gatggttggc aaagatccgc gctctgatat
    24121 cgcgctgatc caaatccaga acccgaaaaa actgaccgca attaagatgg cggattctga
    24181 tgcactgcgc gtgggtgatt acaccgtagc gattggtaac ccgtttggtc tgggcgagac
    24241 ggtaacttcc gggattgtct ctgcgctggg gcgtagcggc ctgaatgctg aaaactacga
    24301 aaacttcatc cagaccgatg cagcgatcaa ccgtggtaac tccggtggtg cgctggttaa
    24361 cctgaacggc gaactgatcg gtatcaacac cgcgatcctc gcaccggacg gcggcaacat
    24421 cggtatcggt tttgctatcc cgagtaacat ggtgaaaaac ctgacctcgc agatggtgga
    24481 atacggccag gtgaaacgcg gtgagctggg tattatgggg actgagctga attccgaact
    24541 ggcgaaagcg atgaaagttg acgcccagcg cggtgctttc gtaagccagg ttctgcctaa
    24601 ttcctccgct gcaaaagcgg gcattaaagc gggtgatgtg atcacctcac tgaacggtaa
    24661 gccgatcagc agctttgccg cactgcgtgc tcaggtgggt actatgccgg tgggcagcaa
    24721 actgaccctg ggcttactgc gcgacggtaa gcaggttaac gtgaacctgg aactgcagca
    24781 gagcagccag aatcaggttg attccagctc catcttcaac ggcattgaag gcgctgagat
    24841 gagcaacaaa ggcaaagatc agggcgtggt agtgaacaac gtgaagacgg gcactccggc
    24901 tgcgcagatc ggcctgaaga aaggtgatgt gattattggt gcgaaccagc aggcagtgaa
    24961 aaacatcgct gaactgcgta aagttctcga cagcaaaccg tctgtgctgg cactgaacat
    25021 tcagcgcggc gacagcacca tctacctgtt aatgcagtaa tctccctcaa ccccttcctg
    25081 aatacgggaa ggggttctcc ttacaatctg tgaacttcgc cacaactcca tacatcttca
    25141 tcatcctttg ggcatttgca caatgccgta cgttacgtac ttccttatgc taagccgtgc
    25201 ataacggagg acttatggct ggctggcatc ttgataccaa aatggcgcag gatatcgtgg
    25261 cacgtaccat gcgcatcatc gataccaata tcaacgtaat ggatgcccgc gggcgaatta
    25321 tcggcagcgg cgatcgtgag cgtattggtg aattgcacga aggtgcattg ctggtacttt
    25381 cacagggacg agtcgtcgat atcgatgacg cggtagcacg tcatctgcac ggtgtgcggc
    25441 aggggattaa tctaccgtta cggctggaag gtgaaattgt cggcgtaatt ggcctgacag
    25501 gtgaaccaga gaatctgcgt aaatatggcg aactggtctg catgacggct gaaatgatgc
    25561 tggaacagtc gcggttgatg cacttgttgg cgcaggatag ccgtttgcgg gaagaactgg
    25621 tgatgaacct gattcaggca gaggagaata ctcccgcact tctgaatggg cgcaacggct
    25681 ggggatcgat ctcaatcaac cgcgagtggt ggctattgtt gaggtcgaca gcggtcagct
    25741 tggcgtggac agcgcaatgg cggagttaca acaactgcaa aacgcgctga ctacgcccga
    25801 gcgtaataat ctggtggcga ttgtctcgct aaccgaaatg gtggtgttga aaccggcgtt
    25861 gaactctttt gggcgctggg atgcagaaga tcatcgtaag cgagttgaac aactgattac
    25921 ccgcatgaaa gagtacggcc agctgcgttt tcgcgtttca ctgggcaact attttaccgg
    25981 tcctggcagt attgcccgat cctatcgtac ggcgaaaacg acgatggtgg tgggtaaaca
    26041 gcggatgcca gaaagtcgct gctattttta tcaggatctg atgttacctg tgttactcga
    26101 cagtttgcgt ggcgactggc aggccaacga actggcgcga ccgctggcgc ggctgaaagc
    26161 gatggacaat aacggcttgc tgcgacgaac gctggcggcg tggtttcgcc acaatgtgca
    26221 accgctggca acgtcaaagg cgttgtttat tcatcgtaat accctggagt atcggcttaa
    26281 tcgtatatcg gaactgaccg ggcttgattt gggcaatttt gatgacaggt tgctgctgta
    26341 tgtggcgtta caactggatg aagagcggta gattatgcgt taaggttgag gcggcgctgg
    26401 ctcattcccc tcagcccgaa ggggagaggg gaatgcccga gttaaccgtc aattcgggat
    26461 tatttacgcg ttagtttttc cagatcggct tcaatctcgc tgatcttatt ggttacgact
    26521 gactccaggt gacgtaagtc gtcgaggatc ttacgcttaa gatcaacttc gctgcggtca
    26581 cgctgacaga tttgatcaag ctcatcaatg atataccgta gattcgggct gatttcctgg
    26641 acttctttat aaccctgacc cacaccatca gcgacgaccg ttttacgctg acgcggatat
    26701 ttaaacttaa cgctcttggc gaaaaactcg cctttgtctt tctggaaata gattttcaga
    26761 atatcgttgt tggcttcctg ccggaggctg taacgatcaa tttcttcagg attggtaata
    26821 cccagacttt tcagattgtc gtacatagcg ttacctcaaa atgagtcagt aaattgtgct
    26881 tattttagca tttggcttgg cccgcccggc tcgactgtga tcgcagaaag cctttttctt
    26941 ccagacttct atcatggcgt aaaaatcaaa aaattacctg ctttattgtg gcgatattcc
    27001 atgggatgga ttagcacatt tatcttcgtt ttgcgaatta catgacagtc cgaaacaaga
    27061 tgaaaaagta tttaaaaaag ggggaggaag aatgagtagg acgaattatt ctgagccaga
    27121 aagcatacgg gcagcatcat gctgcccgta tatgtactct taatcgatgg tacgcagcag
    27181 ttcgttaatg ccgactttgc cgcgagtttt cgcgtcaact ttcttaacga taaccgcaca
    27241 gtagaggctg tatttgccat cttttgacgg cagattacct gaaacaacca cagaccccgc
    27301 cggaacgcga ccgtagtgga tttcgccggt ttcacggtcg taaatacggg tgctctgacc
    27361 aatgtatacg cccatagaga tgacggaacc ttcttcgaca atcacccctt caaccacttc
    27421 agagcgtgcg ccaatgaagc agttatcttc aataatggtc gggttagcct gcagcggctc
    27481 cagcacgccg ccgatgccca cgccaccgga aaggtggacg tttttaccaa tctgcgcaca
    27541 agaaccgacg gtcgcccagg tatcaaccat ggtgccttca tcaacatatg cgccgatgtt
    27601 aacgtaagac ggcatcagca cggtgttacg ggcaataaac gcaccctgac gtaccgccgc
    27661 tggtggcaca acgcggaagc cttctttctg gaaacgtgct tcgtcgtagt cggcgaattt
    27721 catcggcact ttgtcgaagt agcggctttc tgccccttcg atcacctgat tatcattaat
    27781 acggaaagag agcagcaccg cttttttcaa ccactgatgc gtcacccact gaccgtcaat
    27841 tttttccgct acacgcagtg cgccggaatc cagcagggcg atcacctgat ttaccgcttc
    27901 gcgggtaacg gtgtctgcat tggctggcgt gatctcggca cggcgttcaa aagcggtttc
    27961 aataatgttc tgtaactgct gcattgttaa actcttttca tatcagtaaa cacatcaccc
    28021 tttatcgttt ggattgaggg cctctgtcaa ccgctgatgc acttcctgct gcaactcgtt
    28081 attaagcgca cgccggtcag cggtggcaat tatgaataaa tcttctactc gctcgccaat
    28141 ggttgtaatt cgggcaccat gaagcgaaat tcccagatcg gcaaaaattt tcccgactcg
    28201 cgccagcagt ccaggttggt cgagggcgat cagttcgagg aacgatttgc ggtcggtatg
    28261 ggtcggcaaa aacgttactt cggtttcaac agtaaaatgg cgtaatttgg cgggttggcg
    28321 acggggctgc ggtggctgcc agctactttg cgtcagcacc tgctccagac caaaccgaat
    28381 aacctcatgg cgatctgcgg acagcgggct gccatcgggt tccagcacga taaaggtatc
    28441 catcgccata ccgtcgcgag tggtgaaaat ttgtgcgtcg tgaacactta aattgcggcg
    28501 gtctaattcg gcacagacgg cggcaaacag ataggggcgg tccgggctcc agataaaaat
    28561 ctcggtgcct ccacgcgtag cctgcgggct aagcaatacc agcggtttgc ttaaatcatg
    28621 ctgtaataaa tggcgggcat gccaggccag ttgatttggg ctatggcgga caaaatagtt
    28681 agcacgacag cgtgaccaaa tttggtgcag cgcctcttcg tcgatgttat ccatgcgcag
    28741 tagtgccagt gcctggagtt ggtgatggcg aacccgttcg cgcatatccg gcgtgttttg
    28801 cattccgcgt cgtagctgct tttcggtggc aaaatagagt tcgcgcaaca gactttgctt
    28861 ccagctattc cacagtgttt cgttggtggc gcaaatgtca gccactgtca ggcataccag
    28921 atagcgcaga cgattttccg tttgaacttc ttcggcaaac tgcttgatga cttcagggtc
    28981 ctgaatatcg cggcgttgtg ctgtcaccga catcagcaag tgctggcgaa ccagccaggc
    29041 aaccagctgt gtttcgcgtg agttcagccc gtggagttct gcaaaatgca ctacatcctg
    29101 agcaccgaga atggagtggt cgccgccgcg tcctttggcg atatcgtgaa acagcgcggc
    29161 gatgaaaatc agctcagttg acggcaggcg cggccagacg tccacacaca acggatggcg
    29221 ctggcgcgtt tcttcactgg caaaactctc cagtttcagc atcacgcgga tagtatgttc
    29281 atccaccgtg taggcgtgga acagatcaaa ctgcatctgc ccgacgatat gcgaccattg
    29341 cggcatatac gcgccgagca cgctatggcg atgcattggc aatagtccgc gccgcaccgc
    29401 tccggggtga cgcagaatgc tcaaaaacag ttttcgtgct tgcggaatat tacacagcgg
    29461 ttgttgcaga tggcgacggg catggcgtaa ctggcgcagc gtggtggagt aaatgccggt
    29521 gatcgcactg ttgcgcacca tggtgtagaa catacgcaag atggcttccg gctggcgcat
    29581 aaatagtgtt tcatcacgca ggtcgattag cgtaccgcgt agctgaaact catcgtcgag
    29641 tggacgtggt ttttcgtcgg cgggaagggc gaggatggct tcatcgaaca gttgcagcag
    29701 catctggttg agttcactga cgcggcgtgt aacgcggaag taatccttca tcatccgctc
    29761 gaccggttcg ttgccttcac cactgtaatt cagacgctga gcgacgctaa gttggcgatc
    29821 gaataacaga cgattatcgt aacggctgac gaccagatgc agggcaaagc gaatacgcca
    29881 cagtatatgc agacattcgt ttaattccgc ccgctccgct gaggttaaga agccaaaccc
    29941 gaccatttca tccagcgatg ttgcgccaaa atgacggcgg gccacccatt gcagagtgtg
    30001 aatatcgcgc aagccgccag ggctactttt gatgtctggt tcaaggttgt agctggtgcc
    30061 atggtaacgc tgatggcgct ggttctgttc ttcaactttc gctgcgtaga acttgtcgga
    30121 aggccagaat ccttcgctaa aaatatgttt ttgcagttcg aggaagagcg caacatcgcc
    30181 aattaataag cgggattcga ttaaattggt ggcgacggtt aaatccgata acccttccaa
    30241 catgcactct tcaagcgtgc gcacactatg accgacttcc agctttacat cccagagcag
    30301 cgttaacagc tcgcccactt tttgcgcctg atcgtccggg agctttttac ggcttaaaat
    30361 cagtaaatcg atgtcagaaa gtggatgcag ctcgccacga ccgtagccac cgacggcaac
    30421 caatgccagg tcggcaatct ggctgaatcc cgcttcaatc cataatcgtt gcaggagctg
    30481 gtctatgaac tcggtacgtg cctcaatcaa ctgttctgca gagatcccat tgtcaaaggc
    30541 atcacccagc caacgctgga aagtatcgat atgggctttt atcccacaga cggttaattc
    30601 atcacggggc caggcgcatg gattttgcgg ttgaccgggc agggtggaga gagcggtgtt
    30661 tgcgtactgt tctggaaggg tattcattgt gcgccaccca taagattaaa ttatcgcatt
    30721 aaaaaagccg gcattcgccg gcttcatctt attcgtcgtg cgagattatc gccgggatgg
    30781 tgtcatcctt gcgtagcgtc aaaatttcgc agccgttatc agtcaccaca atagtatgct
    30841 catattgtgc agacaagctg cgatctttgg tttttaccgt ccagccatct ttcatggtgc
    30901 ggatctcttt tttacccgcg ttgaccattg gctcaatggt gaacgtcatc cccggtttca
    30961 gcacgacgtt ggtttcacgg gagtcatagt gcagcacctg cggttcttca tggaagccgc
    31021 gaccaatacc gtgtccgcaa tattcacgaa cgacggagaa gccttctgct tcgacaaatt
    31081 tctgaatcgc cgcaccgatt tcgcgcagat taatgcctgg ttttaccatg cgtagcgcca
    31141 ggtacaggct ttcttgcgtg atgcggcaca ggcgttcgcc catgatggtc ggcttaccga
    31201 cgataaacat tttcgaggta tcgccgtgga aaccatcttt gattacggtg acatcaatgt
    31261 taacgatatc gccatctttc agcagcttag catcgtccgg gataccgtgg cacaccactt
    31321 cattaataga gatgcaaacg gatttcggat agccgtgata gccgaggcag gcagaaaccg
    31381 cgtgttgttc attaacaatg taatcattac agatgcgatc cagctcgccg gtgctgacgc
    31441 ccggtttaac atacggttcg atcatctcca gcacttcggc agccagtcgg ccagcgacgc
    31501 gcattttttc gatatcttct ggggtcttga ttgagatagc cattaattct gtccatcagc
    31561 gtcggtgata ccgacaatat atatgtaagt gccgtcaatg gtatcacacc cgggcaaatt
    31621 gagaatcatt ctgaatttcg ccaaacgtgc cactgaaggt tttctataat agaaaattcg
    31681 acgtctgatg ctgtacacag cgccaacaat tattggtgtc cacgacgtat ttgtggtata
    31741 aagcgcgccg gacttccgat ccatttcgta tacacagact ggacggaagc gacaatctca
    31801 ctttgtgtaa caacacacac gtatcggcac atattccggg gtgccctttg gggtcggtaa
    31861 tatgggatac gtggaggcat aaccccaact tttatataga ggttttaatc atggcaactg
    31921 tttccatgcg cgacatgctc aaggctggtg ttcacttcgg tcaccagacc cgttactgga
    31981 acccgaaaat gaagccgttc atcttcggtg cgcgtaacaa agttcacatc atcaaccttg
    32041 agaaaactgt accgatgttc aacgaagctc tggctgaact gaacaagatt gtttctcgca
    32101 aaggtaaaat ccttttcgtt ggtactaaac gcgctgcaag cgaagcggtg aaagacgctg
    32161 ctctgagctg cgaccagttc ttcgtgaacc atcgctggct gggcggtatg ctgactaact
    32221 ggaaaaccgt tcgtcagtcc atcaaacgtc tgaaagacct ggaaactcag tctcaggacg
    32281 gtactttcga caagctgacc aagaaagaag cgctgatgcg cactcgtgag ctggagaaac
    32341 tggaaaacag cctgggcggt atcaaagaca tgggcggtct gccggacgct ctgtttgtaa
    32401 tcgatgctga ccacgaacac attgctatca aagaagcaaa caacttgggt attccggtat
    32461 ttgctatcgt tgataccaac tctgatccgg acggtgttga cttcgttatc ccgggtaacg
    32521 acgacgcaat ccgtgctgtg accctgtacc tgggcgctgt tgctgcaacc gtacgtgaag
    32581 gccgttctca ggatctggct tcccaggcgg aagaaagctt cgtagaagct gagtaataag
    32641 gcttgataac tcccccaaaa tagttcgagt tgcagaaagg cggcaagctc gagaattccc
    32701 gggagcttac atcagtaagt gaccgggatg agcgagcgaa gacaacgcat ctgcggcgcg
    32761 aaatatgaag ggggagagcc cttatagacc aggtagtaca cgtttggtta gggggcctgc
    32821 atatggcccc ctttttcact tttatatctg tgcggtttaa tgccgggcag atcacatctc
    32881 cgaggatttt agaatggctg aaattaccgc atccctggta aaagagctgc gtgagcgtac
    32941 tggcgcaggc atgatggatt gcaaaaaagc actgactgaa gctaacggcg acatcgagct
    33001 ggcaatcgaa aacatgcgta agtccggtgc tattaaagca gcgaaaaaag caggcaacgt
    33061 tgctgctgac ggcgtgatca aaaccaaaat cgacggcaac tacggcatca ttctggaagt
    33121 taactgccag actgacttcg ttgcaaaaga cgctggtttc caggcgttcg cagacaaagt
    33181 tctggacgca gctgttgctg gcaaaatcac tgacgttgaa gttctgaaag cacagttcga
    33241 agaagaacgt gttgcgctgg tagcgaaaat tggtgaaaac atcaacattc gccgcgttgc
    33301 tgcgctggaa ggcgacgttc tgggttctta tcagcacggt gcgcgtatcg gcgttctggt
    33361 tgctgctaaa ggcgctgacg aagagctggt taaacacatc gctatgcacg ttgctgcaag
    33421 caagccagaa ttcatcaaac cagaagacgt atccgctgaa gtggtagaaa aagaatacca
    33481 ggtacagctg gatatcgcga tgcagtctgg taagccgaaa gaaatcgcag agaaaatggt
    33541 tgaaggccgc atgaagaaat tcaccggcga agtttctctg accggtcagc cgttcgttat
    33601 ggaaccaagc aaaactgttg gtcagctgct gaaagagcat aacgctgaag tgactggctt
    33661 catccgtttc gaagtgggtg aaggcatcga gaaagttgag actgactttg cagcagaagt
    33721 tgctgcgatg tccaagcagt cttaattatc aaaaaggagc cgcctgaggg cggcttcttt
    33781 ttgtgcccat cttgtaaatt cagctaaccc ttgtggggct gcgctgaaaa gcgacgtaca
    33841 atgtcgctag tattaattca tttcaatcgt tgacagtctc aggaaagaaa catggctacc
    33901 aatgcaaaac ccgtctataa acgcattctg cttaagttga gtggcgaagc tctgcagggc
    33961 actgaaggct tcggtattga tgcaagcata ctggatcgta tggctcagga aatcaaagaa
    34021 ctggttgaac tgggtattca ggttggtgtg gtgattggtg ggggtaacct gttccgtggc
    34081 gctggtctgg cgaaagcggg tatgaaccgc gttgtgggcg accacatggg gatgctggcg
    34141 accgtaatga acggcttggc aatgcgtgat gcactgcacc gcgcctatgt gaacgctcgt
    34201 ctgatgtccg ctattccatt gaatggcgtg tgcgacagct acagctgggc agaagctatc
    34261 agcctgttgc gcaacaaccg tgtggtgatc ctctccgccg gtacaggtaa cccgttcttt
    34321 accaccgact cagcagcttg cctgcgtggt atcgaaattg aagccgatgt ggtgctgaaa
    34381 gcaaccaaag ttgacggcgt gtttaccgct gatccggcga aagatccaac cgcaaccatg
    34441 tacgagcaac tgacttacag cgaagtgctg gaaaaagagc tgaaagtcat ggacctggcg
    34501 gccttcacgc tggctcgtga ccataaatta ccgattcgtg ttttcaatat gaacaaaccg
    34561 ggtgcgctgc gccgtgtggt aatgggtgaa aaagaaggga ctttaatcac ggaataattc
    34621 ccgtgatgga taaataaggg taagattccg cgtaagtatc gcgggggcgt aagtctggtt
    34681 ataaggcgtt attgttgcag gcagtttggt cacggccagc gcgcagcaac cggagcgtac
    34741 aaaagtacgt gaggatggcg agcactgctc ggggccaaaa tggcaaataa aatagcctaa
    34801 taatccagac gattacccgt aatatgttta atcagggcta tacttagcac acttccactg
    34861 tgtgtgactg tctggtctga ctgagacaag ttttcaagga ttcgtaacgt gattagcgat
    34921 atcagaaaag atgctgaagt acgcatggac aaatgcgtag aagcgttcaa aacccaaatc
    34981 agcaaaatac gcacgggtcg tgcttctccc agcctgctgg atggcattgt cgtggaatat
    35041 tacggcacgc cgacgccgct gcgtcagctg gcaagcgtaa cggtagaaga ttcccgtact
    35101 ctgaaaatca acgtgtttga tcgttcaatg tctccggccg ttgaaaaagc gattatggcc
    35161 tccgatctcg gcctgaaccc gaactctgcg ggtagcgaca tccgtgttcc gctgccgccg
    35221 ctgacggaag aacgtcgtaa agatctgacc aaaatcgttc gtggtgaagc agaacaagcg
    35281 cgtgttgcag tacgtaatgt gcgtcgtgac gcgaacgaca aagtgaaagc actgttgaaa
    35341 gataaagaga tcagcgaaga cgacgatcgc cgttctcagg acgatgtaca gaaactgact
    35401 gatgctgcaa tcaagaaaat tgaagcggcg ctggcagaca aagaagcaga actgatgcag
    35461 ttctgatttc ttgaacgaca aaaacgccgc tcagtagatc cttgcggatc ggctggcggc
    35521 gttttgcttt ttattctgtc tcaactctgg atgtttcatg aagcaactca ccattctggg
    35581 ctcgaccggc tcgattggtt gcagcacgct ggacgtggtg cgccataatc ccgaacactt
    35641 ccgcgtagtt gcgctggtgg caggcaaaaa tgtcactcgc atggtagaac agtgcctgga
    35701 attctctccc cgctatgccg taatggacga tgaagcgagt gcgaaacttc ttaaaacgat
    35761 gctacagcaa caggggagcc gcaccgaagt cttaagtggg caacaagccg cttgcgatat
    35821 ggcagcgctt gaggaggttg atcaggtgat ggcagccatt gttggtgctg ctgggctgtt
    35881 acctacgctt gctgcgatcc gcgcgggtaa aaccattttg ctggccaata aagaatcact
    35941 ggttacctgc ggacgtctgt ttatggacgc cgtaaagcag agcaaagcgc aattgttacc
    36001 ggtcgatagc gaacataacg ctatttttca gagtttaccg caacctatcc agcataatct
    36061 gggatacgct gaccttgagc aaaatggcgt ggtgtccatt ttacttaccg ggtctggtgg
    36121 ccctttccgt gagacgccat tgcgcgattt ggcaacaatg acgcccgatc aagcttgccg
    36181 tcatccgaac tggtcgatgg gacgtaaaat ttctgtcgat tcggctacca tgatgaacaa
    36241 aggtctggaa tacattgaag cgcgttggct gtttaacgcc agcgccagcc agatggaagt
    36301 gctgattcac ccgcagtcag tgattcactc aatggtgcgc tatcaggacg gcagtgttct
    36361 ggcgcagctg ggggaaccgg atatgcgtac gccaattgcc cacaccatgg catggccgaa
    36421 tcgcgtgaac tctggcgtga agccgctcga tttttgcaaa ctaagtgcgt tgacatttgc
    36481 cgcaccggat tatgagcgtt atccatgcct gaaactggcg atggaggcgt tcgaacaagg
    36541 ccaggcagcg acgacagcat tgaatgccgc aaacgaaatc accgttgctg cttttcttgc
    36601 gcaacaaatc cgctttacgg atatcgctgc gttgaattta tccgtactgg aaaaaatgga
    36661 tatgcgcgaa ccacaatgtg tggacgatgt gttatctgtt gatgcgaacg cgagtgaagt
    36721 cgccagaaaa gaggtgatgc gtctcgcaag ctgaggataa tccggctaca gagagtcgcg
    36781 ctatttgtta gcgtagggct tcagtgatat agtctgcgcc atctgatcgt aagtagttgg
    36841 ctttataagg tcaggtatgc cgtggtttta cacggctttt ttttgtatag gcttcagtat
    36901 tcctgagtac cgtaaaccct gtcagggaat aaaaaacgcg tgatgttgtc tgctactcaa
    36961 ccacttagcg aaaaattgcc agcgcatggc tgccgtcatg ttgcgatcat tatggacggc
    37021 aatggtcgct gggcaaaaaa gcaagggaag attcgtgcct ttgggcataa agccggggca
    37081 aaatccgtcc gccgggctgt ctcttttgcg gccaacaacg gtattgaggc gttaacgctg
    37141 tatgccttta gtagtgaaaa ctggaaccga ccagcgcagg aagtcagtgc gttaatggaa
    37201 ctgtttgtgt gggcgctcga tagcgaagta aaaagtctgc accgacataa cgtgcgtctg
    37261 cgtattattg gcgataccag tcgttttaac tcgcgtttgc aagaacgtat tcgtaaatct
    37321 gaagcgctaa cagccggaaa taccggtctg acgctgaata ttgcggcgaa ctacggtgga
    37381 cgttgggata tagtccaggg agtcaggcaa ctggctgaaa aggtgcagca aggaaacctg
    37441 caaccagatc agatagatga agagatgtta aaccagcatg tctgtatgca tgaactggcc
    37501 cctgtagatt tagtaattag gactggggga gagcatcgca ttagtaactt tttgctttgg
    37561 caaattgcct atgccgaact ttactttaca gatgttctct ggcccgattt cgatgaacaa
    37621 gactttgaag gggcgttaaa tgcctttgct aatcgagagc gtcgtttcgg cggcaccgag
    37681 cccggtgatg aaacagcctg atgggggtcg cttttgctga agtatcgcct gatatctgct
    37741 tttgtgttaa tacccgtcgt catcgcggcg ttgtttctgt tgccgccggt ggggttcgcc
    37801 attgtaacgc tggtggtctg catgctggca gcgtgggaat ggggacagct tagcggtttt
    37861 accactcgtt cgcagcgagt atggttggcg gtgttatgcg ggttattgtt ggcgctgatg
    37921 ctttttctgt tgccggaata tcaccgaaat attcatcaac cgctggttga aatctcactt
    37981 tgggcttcgt tgggttggtg gattgtcgcg ctattgctgg tgctgtttta cccaggttcc
    38041 gcagcaatct ggcgtaactc taaaacattg cgccttattt ttggcgtgct aaccattgtt
    38101 cccttcttct ggggcatgct ggcgttacgg gcctggcact atgacgagaa tcattacagt
    38161 ggcgcaatat ggctgctcta tgtcatgatc ctggtatggg gcgctgactc cggcgcatat
    38221 atgtttggca aattgtttgg taaacataag ctggcaccga aggtttctcc gggtaaaacc
    38281 tggcaaggct ttataggtgg actcgctact gcagcggtaa tctcatgggg ttatggcatg
    38341 tgggcgaatc tcgacgtcgc tcccgtcacc ttactcattt gctctattgt cgcagcgtta
    38401 gcctcagtgc tcggcgatct gaccgagagt atgtttaagc gtgaagcagg aattaaggac
    38461 agcggtcatt taattccagg acacggtggt attttagatc gtattgatag cctgacggct
    38521 gcggtaccgg tctttgcttg cttgttgtta ctggtattca ggacgcttta acggaaggta
    38581 atatgctgag ttttctctgg gatttggctt cgttcatcgt tgcactgggt gtacttatca
    38641 ccgtgcatga atttggtcat ttctgggttg cccggcgttg tggtgttcgc gttgagcgtt
    38701 tctcaatagg gtttggtaag gcgctctggc ggcgaactga taagctcggc accgaatatg
    38761 ttatcgccct gatcccgttg ggcggttatg tcaaaatgct ggatgagcgc gcagaaccgg
    38821 tcgttccgga actccgccac catgccttca ataataaatc tgtcggccaa cgagcggcga
    38881 ttattgccgc aggtccggtt gcaaacttca tttttgctat ctttgcctac tggctggttt
    38941 ttattattgg tgtgcctggc gtacgtccgg tggttggcga aatagcagcc aattcgatag
    39001 ctgcggaagc acaaattgca ccaggtacgg aactaaaagc cgtagatggt atcgaaacgc
    39061 ctgattggga tgccgtgcgt ttgcagttgg tcgataaaat tggcgatgaa agcaccacca
    39121 ttacagtagc gccatttgac agcgaccaac ggcgggatgt aaagctcgat ttacgtcact
    39181 gggcgtttga gcctgataaa gaagatccgg tatcttcgct ggggattcgt cctcgtgggc
    39241 cgcaaattga acctgtactg gaaaatgtgc agccaaactc ggcggcaagc aaggcaggtt
    39301 tgcaagcagg cgacaggatc gttaaagtcg atggtcagcc cttaacgcag tgggtgacct
    39361 ttgtgatgct tgtccgggat aacccgggta aatccttagc gttagaaatc gaaaggcagg
    39421 ggagtccctt gtctttgaca ttaatcccgg agagtaaacc gggtaatggt aaagcgattg
    39481 gttttgtcgg tattgagccg aaagtcattc ctttgccaga tgagtataaa gttgtacgcc
    39541 agtatgggcc gttcaacgcc atcgtcgaag ccacggacaa aacgtggcag ctgatgaagc
    39601 tgacggtcag tatgctggga aaattgatca ccggtgatgt gaaactgaac aacctcagtg
    39661 ggccgatctc tatcgccaag ggggctggga tgacagcgga actcggggta gtttattacc
    39721 tgccgtttct tgcgcttatt agcgtgaact tagggataat taacctgttt ccgttgcccg
    39781 tacttgacgg ggggcatctg ctgttccttg cgatcgaaaa gatcaagggc ggaccggtat
    39841 ccgagcgggt tcaagacttt tgttatcgca ttggctcgat tctgctggtg ctgttaatgg
    39901 ggcttgcact tttcaatgat ttctctcggt tatgagagtt agttaggaag aacgcataat
    39961 aacgatggcg atgaaaaagt tgctcatagc gtcgctgctg tttagcagcg ccaccgtata
    40021 cggtgctgaa gggttcgtag tgaaagatat tcatttcgaa ggccttcagc gtgtcgccgt
    40081 tggtgcggcc ctcctcagta tgccggtgcg tacaggcgac acggttaatg atgaagatat
    40141 cagtaatacc attcgcgctc tgtttgctac cggcaacttt gaggatgttc gcgtccttcg
    40201 tgatggtgat acccttctgg ttcaggtaaa agaacgtccg accattgcca gcattacttt
    40261 ctccggtaac aaatcggtga aagatgacat gctgaagcaa aacctcgagg cttctggtgt
    40321 gcgtgtgggc gaatccctcg atcgcaccac cattgccgat atcgagaaag gtctggaaga
    40381 cttctactac agcgtcggta aatatagcgc cagcgttaaa gctgtcgtga ccccgctgcc
    40441 gcgcaaccgt gttgacctaa aactggtgtt ccaggaaggt gtgtcagctg aaatccagca
    40501 aattaacatt gttggtaacc atgctttcac caccgacgaa ctgatctctc atttccaact
    40561 gcgtgacgaa gtgccgtggt ggaatgtggt aggcgatcgt aaataccaga aacagaaact
    40621 ggcgggcgat cttgaaaccc tgcgcagcta ctatctggac cgcggttatg cccgtttcaa
    40681 catcgactct acccaggtca gtctgacgcc agataaaaaa ggtatttacg tcacggtgaa
    40741 catcaccgaa ggcgatcagt acaagctttc tggcgttgaa gtgagcggca accttgccgg
    40801 gcactccgct gaaattgagc aactgactaa gatcgagccg ggcgagctgt ataacggcac
    40861 taaagtgacc aaaatggaag atgacattaa aaagcttctc ggtcgctatg gttatgccta
    40921 tccgcgcgta cagtcgatgc ctgaaattaa cgatgccgac aaaaccgtta aattacgcgt
    40981 gaacgttgat gcgggtaacc gtttctacgt gcgtaagatc cgttttgaag gtaacgatac
    41041 ctcgaaagat gccgtcctgc gtcgcgaaat gcgtcagatg gaaggtgcat ggttggggag
    41101 cgatctggtc gatcagggta aggagcgtct gaatcgtctg ggcttctttg aaactgtcga
    41161 taccgatacc caacgtgttc cgggtagccc ggaccaggtt gatgtagtct acaaggtgaa
    41221 agaacgtaac actggtagct tcaacttcgg gattggttac ggtactgaaa gtggcgtgag
    41281 cttccaggct ggcgtgcaac aggataactg gttaggtaca ggttatgctg ttggtatcaa
    41341 cgggaccaaa aacgattacc agacctatgc tgaactgtcg gtaaccaacc cgtacttcac
    41401 cgtagatggc gtaagcctcg gtggtcgtct cttctataat gacttccagg cagatgacgc
    41461 cgacctgtcc gactatacca acaagagtta tggtacagac gtgacgttgg gcttcccgat
    41521 taacgaatat aactcgctgc gtgcaggtct gggttatgta cataactccc tgtccaacat
    41581 gcagcctcag gttgcgatgt ggcgttatct gtactctatg ggtgaacatc cgagcacctc
    41641 tgatcaggat aacagcttca aaacggacga cttcacgttc aactatggtt ggacctataa
    41701 caagcttgac cgtggttact tcccgacaga tggttcacgt gtcaacctga ccggtaaagt
    41761 gaccattcct ggatcggata acgaatacta caaagtgacg ttagacacgg cgacttatgt
    41821 gccgatcgat gacgatcaca aatgggttgt tctgggtcgt acccgctggg gttatggtga
    41881 tggtttaggc ggcaaagaga tgccgttcta cgagaacttc tatgccggtg gttccagcac
    41941 cgtgcgtggc ttccagtcca ataccattgg tccgaaagca gtttacttcc cgcatcaggc
    42001 cagtaattat gatccggact atgattacga atgtgcgact caggacggcg cgaaagacct
    42061 gtgtaaatcg gatgatgctg taggcggtaa cgccatggcg gttgccagcc tcgagttcat
    42121 caccccgacg ccgtttatta gcgataagta tgctaactcg gttcgtactt cattcttctg
    42181 ggatatgggt accgtttggg atacaaactg ggattccagc caatattctg gatatccgga
    42241 ctatagtgat ccaagcaata tccgtatgtc tgcgggtatc gcattacaat ggatgtcccc
    42301 attggggccg ttggtgttct cctacgccca gccgttcaaa aagtacgatg gagacaaggc
    42361 agaacagttc cagtttaaca tcggtaaaac ctggtaagtg ttctccacaa aggaatgtag
    42421 tggtagtgta gcgatgactt taggcgatca atataagatc gccgggccac gcaaagaact
    42481 gcaccctccg gtgcaaatgg gatggtaagg agtttattgt gaaaaagtgg ttattagctg
    42541 caggtcttgg tttagcactg gcaacttctg ctcaggcggc tgacaaaatt gcaatcgtca
    42601 atatgggcag cctgttccag caggtagcgc agaaaaccgg tgtttctaac acgctggaaa
    42661 atgagttcaa aggccgtgcc agcgaactgc agcgtatgga aaccgatctg caggctaaaa
    42721 tgaaaaaact gcagtccatg aaagcgggca gcgatcgcac caaactggaa aaagacgtga
    42781 tggctcagcg ccagactttt gctcagaaag cgcaggcttt tgagcaggat cgcgcacgtc
    42841 gttccaacga agaacgcggc aaactggtta ctcgtatcca gactgctgtg aaatccgttg
    42901 ccaacagcca ggatatcgat ctggttgttg atgcaaacgc cgttgcttac aacagcagcg
    42961 atgtaaaaga catcactgcc gacgtactga aacaggttaa ataagtaatg ccttcaattc
    43021 gactggctga tttagcgcag cagttggatg cagaactaca cggtgatggc gatatcgtca
    43081 tcaccggcgt tgcgtccatg caatctgcac aaacaggtca cattacgttc atggttaacc
    43141 caaaataccg tgagcattta ggcttgtgcc aggcgtccgc ggttgtcatg acccaggacg
    43201 atcttccttt cgcgaaaagt gccgcgctgg tagtgaagaa tccctacctg acttacgcgc
    43261 gcatggcgca aattttagat accacgccgc agcccgcgca gaacattgca cccagtgcgg
    43321 tgatcgacgc gacggcgaag ctgggtaaca acgtatcgat tggcgctaac gcggtgattg
    43381 agtccggcgt tgaactgggc gataacgtga ttatcggtgc cggttgcttc gtaggtaaaa
    43441 acagcaaaat cggtgcaggt tcgcgtctct gggcgaacgt aaccatttac catgagatcc
    43501 agatcggtca gaattgcctg atccagtccg gaacagtggt aggcgcagac ggctttggtt
    43561 atgccaacga tcgtggtaac tgggtgaaga tcccacagat tggtcgcgta attattggcg
    43621 atcgcgtgga gatcggtgcc tgcacaacca tcgatcgcgg cgcgctggat gacactgtta
    43681 ttggcaatgg cgtgatcatt gataaccagt gccagattgc acataacgtc gtgattggcg
    43741 acaatacggc ggttgccggt ggcgtcatta tggcgggcag cctgaaaatt ggtcgttact
    43801 gcatgatcgg cggagccagc gtaatcaacg ggcatatgga aatatgcgac aaagtgacgg
    43861 ttacgggcat gggtatggtg atgcgtccca tcactgaacc aggcgtctat tcctcaggca
    43921 ttccgctgca acccaacaaa gtctggcgca aaaccgctgc actggtgatg aacattgatg
    43981 acatgagcaa gcgtctgaaa tcgcttgagc gcaaggttaa tcaacaagac taacgttcca
    44041 tcttttgttc gccaaacttt acggcctgtc tcattcttac gattgcggca ggccgtgtta
    44101 ttattgtcgt ttcttatatt ttgacaggaa gagtatcttg actactaaca ctcatactct
    44161 gcagattgaa gagattttag aacttctgcc gcaccgtttc ccgttcttac tggtggatcg
    44221 cgtgctggat tttgaagaag gtcgttttct gcgcgcagta aaaaatgtct ctgtcaatga
    44281 gccattcttc cagggccatt tccctggaaa accgattttc ccgggtgtgc tgattctgga
    44341 agcaatggca caggcaacag gtattctggc gtttaaaagc gtaggaaaac tggaaccggg
    44401 tgagctgtac tacttcgctg gtattgacga agcgcgcttc aagcgcccgg tcgtgcctgg
    44461 cgatcaaatg atcatggaag tcactttcga aaaaacgcgc cgcggcctga cccgttttaa
    44521 aggggttgct ctggtcgatg gtaaagtagt ttgcgaagca acgatgatgt gtgctcgtag
    44581 ccgggaggcc tgatacgtga ttgataaatc cgcctttgtg catccaaccg ccattgtgga
    44641 agagggcgcg tcaattggcg cgaacgcaca cattggtcct ttttgtatcg ttggacccca
    44701 tgtcgaaatt ggtgagggta ccgtactgaa atctcacgtt gtcgtgaatg gtcatactaa
    44761 aattggccgc gataatgaga tttatcagtt tgcctccatc ggcgaagtta accaggattt
    44821 gaaatatgct ggcgaaccga cccgtgtgga aatcggcgat cgtaaccgca ttcgcgaaag
    44881 cgtcaccatt catcgtggca cagtccaggg cggtggattg acgaaggtgg gcagcgacaa
    44941 cttactgatg atcaacgcgc acattgcgca cgattgtacg gtaggtaacc gctgtattct
    45001 cgccaacaac gcaacgctgg cgggtcacgt atcggttgac gacttcgcga tcatcggcgg
    45061 catgaccgca gtccatcagt tctgcatcat tggtgcgcat gtgatggttg gcggctgctc
    45121 cggtgtggcg caggacgtcc ctccttatgt tattgcgcag ggtaaccacg caacgccgtt
    45181 cggtgtcaat atcgaagggc tgaagcgccg cggattcagc cgtgaggcga ttaccgctat
    45241 ccgcaatgcg tataagctaa tttatcgtag cggtaaaacg ctcgatgaag tgaaaccgga
    45301 aattgctgaa ctggcggaaa catatccgga agtgaaagcc tttaccgatt tctttgcacg
    45361 ctcaacgcgc ggtctgattc gttaatgact gaccagcgtc cattaacgat tgccctggtc
    45421 gccggagaaa cctccggcga tatcctgggg gccggtttaa tccgcgctct gaaagaacgt
    45481 gtgcccaacg cccgctttgt tggtgttgcc gggccacgaa tgcaggctga aggctgcgaa
    45541 gcctggtacg aaatggaaga actggcggtg atgggcattg ttgaagtgct cggtcgtctg
    45601 cgtcgcttac tgcatattcg tgccgatctg acaaagcgtt ttggcgaact gaagccagat
    45661 gtttttgttg gtattgatgc gcccgacttc aatattactc ttgaaggtaa cctcaaaaag
    45721 cagggtatca aaaccattca ttacgtcagt ccatccgtct gggcgtggcg acagaaacgc
    45781 gttttcaaaa taggcagagc caccgatctg gtgctcgcat ttctgccttt cgaaaaagcg
    45841 ttttatgaca aatacaatgt accgtgccgc tttatcggtc ataccatggc tgatgccatg
    45901 ccgttagatc cagataaaaa tggtgcccgt gatgtgctgg ggatccctta cgatgcccac
    45961 tgtctggcat tgttgccggg cagccgtggt gcagaagtcg aaatgcttag tgccgatttc
    46021 ttgaaaactg cccagctttt gcgccagaca tatccggatc tcgaaatcgt ggtgccgctg
    46081 gtgaatgcca aacgccgcga gcagtttgaa cgcatcaaag ctgaagtcgc gccagacctg
    46141 tcggttcatt tgctggatgg aatgggccgt gaggcgatgg tcgccagcga tgcggcacta
    46201 ctggcgtcgg ggacggcagc cctggagtgt atgctggcga aatgcccgat ggtggtggga
    46261 tatcgcatga agccttttac cttctggttg gcgaagcggc tggtgaaaac tgattatgtc
    46321 tcgctgccaa atctgctggc gggcagagag ttagtcaaag agttattgca ggaagagtgt
    46381 gagccgcaaa aactggctgc ggcgctgtta ccgctgttgg cgaacgggaa aaccagccac
    46441 gcgatgcacg ataccttccg tgaactgcat cagcagatcc gctgcaatgc cgatgagcag
    46501 gcggcacaag ccgttctgga gttagcacaa tgatcgaatt tgtttatccg cacacgcagc
    46561 tggttgcggg tgtggatgaa gtcggacgcg ggccgttagt tggcgcggtc gtcaccgctg
    46621 cggtgatcct tgacccggcg cgcccgattg ccgggctgaa tgattccaaa aagctgagcg
    46681 aaaaacgccg tctggcgctc tatgaagaga tcaaagagaa agcgttgagc tggagtctgg
    46741 gccgcgcgga accccacgaa atcgacgagc tgaacattct tcatgcgacc atgctggcga
    46801 tgcagcgtgc cgtcgctggg ctgcatattg cgccggaata tgtgttgatt gatggtaacc
    46861 gctgcccgaa attaccgatg cctgcgatgg ctgtggtgaa aggggatagc cgcgtaccag
    46921 aaatcagtgc cgcgtctatc ctggcgaaag tgacgcgtga cgccgaaatg gcggcgctgg
    46981 atattgtttt cccgcaatat ggttttgccc aacataaagg gtatccaacc gcttttcatc
    47041 tggaaaaact ggctgaatat ggcgcgactg aacatcatcg tcgcagcttt gggcctgtca
    47101 aacgcgcact gggacttgcg tcctgattct tgtgtcgaga ttaagtaaac cggaatctga
    47161 agatgtctga accacgtttc gtacacctgc gggtgcacag cgactactcg atgatcgatg
    47221 gcttggccaa aaccgcaccg ctggtaaaaa aggcggcggc gttgggtatg ccagcactgg
    47281 cgatcaccga tttcaccaac ctttgcggtc tggtgaagtt ctacggagcg ggacatggcg
    47341 cagggattaa gcctatcgtc ggggcagatt ttaatgtcca gtgcgacctg ctgggtgatg
    47401 agttaaccca cctgacggta ctggcggcga acaataccgg ctatcagaat ctggcgttgc
    47461 tgatctcaaa agcgtatcag cgcgggtacg gtgccgccgg gccgatcatc gatcgcgact
    47521 ggcttatcga attaaacgaa gggttgatcc ttctttccgg cgggcgcatg ggcgacgtcg
    47581 gacgcagtct tttgcgtggt aacagcgcgc tggtagatga gtgtgtcgcg ttttatgaag
    47641 aacacttccc ggatcgctat tttctcgagc tgatccgcac cggcaggccg gatgaagaaa
    47701 gctatctgca cgcggcggtg gaactggcgg aagcgcgcgg tttgcccgtc gtggcgacta
    47761 acgatgtgcg ctttatcgac agcagcgact ttgacgcaca cgaaatccgc gtcgcgatcc
    47821 acgacggctt taccctcgac gatcctaaac gcccgcgtaa ctattcgccg cagcaatata
    47881 tgcgtagcga agaggagatg tgtgagctgt ttgccgacat cccagaagcc cttgccaaca
    47941 ccgttgagat cgccaaacgc tgtaacgtaa ctgtgcgtct tggtgaatac ttcctgccgc
    48001 agttcccgac cggggacatg agcaccgaag attatctggt caagcgtgca aaagaaggcc
    48061 tggaagagcg tctggcattt ttattccctg acgaggaaga acgtcttaaa cgccgcccgg
    48121 aatatgacga acgtctggag actgaacttc aggttatcaa ccagatgggc ttcccgggct
    48181 acttcctcat cgttatggaa tttatccagt ggtcgaaaga taacggcgta ccggtagggc
    48241 caggccgtgg ctccggtgcg ggttcactgg tggcctacgc gctgaaaatc accgacctcg
    48301 atccgctgga atttgacctg ctgttcgaac gtttccttaa cccggaacgt gtctccatgc
    48361 ctgacttcga cgttgacttc tgtatggaga aacgcgatca ggttatcgaa cacgtggcgg
    48421 acatgtacgg tcgtgatgcg gtatcgcaga ttatcacctt cggtacgatg gcggcgaaag
    48481 cggtgatccg cgacgtaggc cgcgtgctgg ggcatccgta cggctttgtc gaccgtatct
    48541 cgaaactgat cccgcccgat ccggggatga cgctggcgaa agcgtttgaa gccgagccgc
    48601 agctgccgga aatctacgaa gcggatgaag aagttaaggc gctgatcgac atggcgcgca
    48661 aactggaagg ggtcacccgt aacgccggta agcacgccgg tggggtggtt atcgcgccga
    48721 ccaaaattac cgattttgcg ccgctttatt gcgatgaaga gggcaaacat ccggtcaccc
    48781 agtttgataa aagcgacgtt gaatacgccg gactggtgaa gttcgacttc cttggtttgc
    48841 gtacgctcac catcatcaac tgggcgctgg agatgatcaa caagcggcgg gcgaagaatg
    48901 gcgagccacc gctggatatc gccgcgatcc cgctggatga caagaaaagc ttcgacatgc
    48961 tgcaacgctc ggaaaccacg gcggtattcc agcttgaatc gcgcggcatg aaggacctga
    49021 tcaagcgtct gcaacctgac tgcttcgaag atatgatcgc actggtggca ctgttccgcc
    49081 ctggtccgtt gcagtcaggg atggtggata actttatcga ccgtaaacat ggtcgtgaag
    49141 agatctccta tccggacgtg cagtggcagc atgaaagcct gaaatcggta ctggagccaa
    49201 cctacggcat tatcttgtat caggaacagg tcatgcagat tgcccaggtg ctttctggtt
    49261 ataccctcgg tggcgcggat atgctgcgcc gtgcgatggg taagaaaaag ccggaagaga
    49321 tggctaagca gcgttctgta tttgctgaag gtgcagaaaa gaacggaatc aacgccgaac
    49381 tggcgatgaa aatcttcgac ctggtggaga aattcgcggg ttacggattt aacaaatcgc
    49441 actctgcggc ctatgctttg gtgtcatatc aaacgttatg gctgaaagcg cactatccgg
    49501 cggagtttat ggcggcggta atgaccgccg atatggacaa caccgagaag gtggtgggcc
    49561 tggtggatga gtgctggcgg atggggctga aaatcctgcc accagatata aactccggtc
    49621 tttaccattt ccacgtcaac gacgatggcg aaatcgtgta tggtattggc gccatcaaag
    49681 gggtaggtga aggtccgatt gaggccatca tcgaagcccg taataaaggc ggctacttcc
    49741 gcgaactgtt tgatctctgc gcccgtaccg acaccaaaaa gttaaaccgg cgggtgctgg
    49801 aaaaactgat catgtccggg gcgtttgacc gtcttgggcc acaccgcgcg gcgctgatga
    49861 actcgctggg tgatgcgtta aaagcggcag atcaacacgc gaaagcggaa gctatcggtc
    49921 aggccgatat gttcggcgtg ctggccgaag agccggaaca aattgaacaa tcctacgcca
    49981 gctgccaacc gtggccggag caggtggtat tagatgggga acgtgaaacg ttaggcctgt
    50041 acctgaccgg acaccctatc aaccagtatt taaaagagat tgagcgttat gtcggaggcg
    50101 taaggctgaa agacatgcac ccgacagaac gtggtaaagt catcacggct gcggggctcg
    50161 ttgttgccgc gcgggttatg gtcaccaagc gcggcaatcg tatcggtatc tgcacgctgg
    50221 atgaccgttc cgggcggctg gaagtgatgt tgtttactga cgccctggat aaataccagc
    50281 aattgctgga aaaagaccgc atacttatcg tcagcggaca ggtcagcttt gatgacttca
    50341 gcggtgggct taaaatgacc gctcgcgaag tgatggatat tgacgaagcc cgggaaaaat
    50401 atgctcgcgg gcttgctatc tcgctgacgg acaggcaaat tgatgaccag cttttaaacc
    50461 gactccgtca gtctctggaa ccccaccgct ctgggacaat tccagtacat ctctactatc
    50521 agagggcgga tgcacgcgcg cggttgcgtt ttggcgcgac gtggcgtgtc tctccgagcg
    50581 atcgtttatt aaacgatctc cgtggcctca ttggttcgga gcaggtggaa ctggagtttg
    50641 actaatacag gaatactatg agtctgaatt tccttgattt tgaacagccg attgcagagc
    50701 tggaagcgaa aatcgattct ctgactgcgg ttagccgtca ggatgagaaa ctggatatta
    50761 acatcgatga agaagtgcat cgtctgcgtg aaaaaagcgt agaactgacg cgtaaaatct
    50821 tcgccgatct cggtgcatgg cagattgcgc aactggcacg ccatccgcag cgtccttata
    50881 ccctggatta cgttcgcctg gcatttgatg aatttgacga actggctggc gaccgtgcgt
    50941 atgcagacga taaagctatc gtcggtggta tcgcccgtct cgatggtcgt ccggtgatga
    51001 tcattggtca tcaaaaaggt cgtgaaacca aagaaaaaat tcgccgtaac tttggtatgc
    51061 cagcgccaga aggttaccgc aaagcactgc gtctgatgca aatggctgaa cgctttaaga
    51121 tgcctatcat cacctttatc gacaccccgg gggcttatcc tggcgtgggc gcagaagagc
    51181 gtggtcagtc tgaagccatt gcacgcaacc tgcgtgaaat gtctcgcctt ggcgtaccgg
    51241 tagtttgtac ggttatcggt gaaggtggtt ctggcggtgc gctggcgatt ggcgtgggcg
    51301 ataaagtgaa tatgctgcaa tacagcacct attccgttat ctcgccggaa ggttgtgcgt
    51361 ccattctgtg gaagagcgcc gacaaagcgc cgctggcggc tgaagcgatg ggtatcatcg
    51421 ctccgcgtct gaaagagctg aaactgatcg actccatcat cccggaaccg ctgggcggcg
    51481 ctcaccgtaa cccggaagcg atggcggcat cgttgaaagc gcaactgctg gcggatctgg
    51541 ccgatctcga tgtgttaagc actgaagatt taaaaaatcg tcgttatcag cgcctgatga
    51601 gctacggtta cgcgtaattc gcaaaagttc tgaaaaaggg tcacttcggt ggcccttttt
    51661 tatcgccacg gtttgagcag gctatgatta aggaaggatt ttccaggagg aacacatgaa
    51721 catcattgcc attatgggac cgcatggcgt cttttataaa gatgagccca tcaaagaact
    51781 ggagtcggcg ctggtggcgc aaggctttca gattatctgg ccacaaaaca gcgttgattt
    51841 gctgaaattt atcgaacata accctcgaat ttgcggcgtg atttttgact gggatgagta
    51901 cagtctcgat ttatgcagcg atatcaatca acttaatgaa tatctcccgc tttatgcctt
    51961 catcaacacc cactcgacga tggatgtcag cgtgcaggat atgcggatgg cgctctggtt
    52021 ttttgaatat gcgctggggc aggcggaaga tatcgccatt cgtatgcgtc agtacaccaa
    52081 cgaatatctt gataacatta cgccgccgtt cacgaaagcc ttgtttacct acgtcaaaga
    52141 gcggaagtac accttttgta cgccggggca tatgggcggc accgcatatc aaaaaagccc
    52201 ggttggctgt ctgttttatg attttttcgg cgggaatacc cttaaggctg atgtctctat
    52261 ttcggtcacc gagcttggtt cgttgcttga ccacaccggg ccacacctgg aagcggaaga
    52321 gtacatcgcg cggacttttg gcgcggaaca gagttatatc gttaccaacg gaatatcgac
    52381 gtcgaacaaa attgtgggta tgtacgccgc gccatccggc agtacgctgt tgatcgaccg
    52441 caactgccat aaatcactgg cgcatctgtt gatgatgaac gatgtagtgc cagtctggct
    52501 gaaaccgacg cgtaatgcgt tggggattct gggggggatc ccgcgccgtg aatttactcg
    52561 cgacagcatc gaagagaaag tcgctgccac tacgcaagca caatggccgg ttcatgcggt
    52621 gatcaccaac tccacctatg atggtttgct ctacaacacc gactggatca aacagacgct
    52681 ggatgtcccg tcgatccact tcgattccgc ctgggtgccg tatactcatt ttcatccaat
    52741 ctaccagggt aaaagtggta tgagcggcga gcgtgttgcg ggaaaagttt tcttcgaaac
    52801 gcaatcgacc cacaaaatgc tggcggcgtt atcgcaggct tcactgatcc acattaaagg
    52861 cgagtatgac gaagaggcgt ttaacgaagc ctttatgatg cataccacca cctcgcccag
    52921 ttatcccatt gttgcttcgg ttaagacggc ggcggcgatg ctgcgtggta atccgggcaa
    52981 acggctgatt aaccgttcgg tggagcgggc gctgcatttt cgcaaagagg tccagcggct
    53041 gcgggaagag tctgacggtt ggtttttcga tatctggcaa ccgccgcagg tggatgaagc
    53101 cgaatgcagg cccgttgcgc ctggcgaaca atggcacggc tttaacgatg cggatgccga
    53161 tcatatgttt ctcgatccgg ttaaagtcac tattttgaca ccggggatgg acgaacaggg
    53221 caatatgagt gaggagggga tcccggcggc gctggtagca aaattcctcg acgaacgtgg
    53281 gatcgtagta gagaaaaccg gcccttataa cctgctgttt ctctttagta ttggcatcga
    53341 taaaaccaaa gcaatgggat tattgcgtgg gttgacggaa tttaagcgct cttacgatct
    53401 caacctgcgg atcaaaaata tgctgcccga tctctatgct gaagatcccg atttctaccg
    53461 caatatgcgt attcaggatc tggcgcaagg gatccataag ctgattcgta aacacgatct
    53521 ttccggtttg atgttgcggg cattcgatac tttgccggaa atgatcatga cgccacatca
    53581 ggcatggcag agacagatta aaggtgaagt agaaaccatt gcgctggaac aactggtcgg
    53641 tagagtatcg gcaaatatga tcctgcctta tccaccgggc gtaccgctgt tgatgccagg
    53701 agaaatgctg accaaagaga gccgcacggt actcgatttt ctactgatgc tttgttccgt
    53761 agggcaacat taccccggtt ttgaaacgga tattcacggc gcgaaacagg acgaagacgg
    53821 cgtttaccgc gtacgagtcc taaaaatggc gggataactt gccagagcgg cttccgggcg
    53881 agtaacgtgc tgttaacaaa taaaggagac gttatgctgg gtttaaaaca ggttcaccat
    53941 attgcgatta ttgcgacgga ttatgcggtg agcaaagctt tctactgcga tattcttggt
    54001 ttcacgctgc aaagcgaagt ctatcgcgaa gcgcgcgact cgtggaaagg ggatttggcg
    54061 cttaatgggc aatatgtgat tgagcttttc tcttttccgt tcccgccgga gcgccccagc
    54121 cgaccggaag cttgcggcct gcgtcatctg gcttttagcg ttgatgacat cgatgcggca
    54181 gtggcgcacc ttgaaagcca taacgtgaag tgtgaagcca tccgtgtcga tccatacacg
    54241 caaaaacgct tcaccttctt taacgatccg gacgggctgc cgttggaact gtatgagcag
    54301 taaggcttgt catcgccgca tttgcccggt aacgtgccgg gcattgctac tgtaaaatcg
    54361 caccatcatg acactcacgc tcaatagaca acttctcacc tcacgccaga ttctggtggc
    54421 ctttagcggc gggcttgact ccaccgttct gctgcatcag ttggtgcagt ggcggacgga
    54481 aaatccgggc gtcactctgc gcgctattca tgtgcatcac ggtttaagtg ccaatgccga
    54541 tgcctgggtt acgcattgcg aaaacgtctg ccaacagtgg caggtgccgc tggtggtcga
    54601 acgcgtacaa cttgcgcaag aggggctggg gattgaggcc caggcgcggc aggcacgtta
    54661 tcaggcattt gcccgtaccc tattgcccgg cgaagtgctg gtcaccgcgc aacatctcga
    54721 cgatcaatgt gaaacctttc tgctggcgct aaaacgcggt agcggccctg ccgggctttc
    54781 ggctatggcg gaagtttcgg agtttgctgg aacacggctt atccgcccgt tgcttgcccg
    54841 cacgcggggg gaactggagc agtgggcatt ggcgcatggt ttacgctgga ttgaagacga
    54901 aagtaatcag gacgactcat acgatcgtaa ctttctgcgc ctgcgcgtag tgccgttatt
    54961 gcagcagcgt tggccgcatt ttgcggaagc aacggcccgc agcgccacac tttgtgctga
    55021 acaagagagt ctgttggatg aactgctggc ggatgattta gcacactgcc aaacgtcgca
    55081 ggggacgctg cagattgcgc caatgctggc gatgagtgat gcccgtcgcg cggcgattat
    55141 tcgccgctgg ctggcagggc agaatgcacc gatgccttcc cgcgacgcgc tggtgcgcat
    55201 ctggcaggaa gtggcgctgg cgcgggaaga tgcttccccc tgtttacgtt tgggcgcctt
    55261 tgaaatccgc cgctatcagt cgcaactgtg gtggattaaa tccgtcaccg ggcaaagcga
    55321 aaccattgtg ctgtggcaga cgtggctaca accgctggaa ttaccggcgg ggctgggaac
    55381 cgtacagctt actgcgggag gcgatattcg ccctccgcgt gcagacgaag cggtcagcgt
    55441 gcgtttcaaa gcgccaggat tgctgcatat tgtcgggcgc aacggcggac gtaagctgaa
    55501 gaaaatctgg caagagttag gcgtgccgcc gtggctacgt gacaccacgc cactgctgtt
    55561 ttatggagaa acgctgattg cggcggcagg ggtatttgtg acgcaagaag gtgtggctga
    55621 aggtgagaat ggcgtaagtt ttgtctggca gaaaacgctt agttaagtga aagccggata
    55681 agacgcatca aacgtcgcat ccggcgaaag tcaatcagga ttcgcttacc accaccgtac
    55741 cgatttcagg gtggctaaag ctggtaattt tatccagacg cagctcgcgg gtttcgccag
    55801 cggcctcgac gaccagatac tccacatttt tgcgggagac taaatcactg gcttttgcct
    55861 gcaatttttc gccatctttc agctcaagtg tcagcattaa atgatgctgg caggcgagct
    55921 caagattatc gtaatcatca caattaattg gttgatacgt atcattcatt gacataatcg
    55981 ctcaccagta agtttgccgc agcgtatgct gctttttccc tgacagcctc agaaagggcg
    56041 tcgtcggcag ccatttcatt cagcactttc aaaacgcagc ccagcgcgtc cggaacgtat
    56101 cctaagtctc cgctggcgat ttccgcgtac cgcttgcgta ttaactcaca atatttttcc
    56161 acatgccctc ctgtcagcac tctgacttaa ccgtggatgc aagtctaagc ctacgaagat
    56221 aaactctgtt tcgcaaggtg actataccac actcatttct gcaatatcag cgccgcaact
    56281 gcacgtattc cgttacaatg gcctcctgat tcgaaaggag ttttcttatg gcgcttaaag
    56341 cgacaattta taaagcgacg gttaatgtgg ccgatctcga ccgcaaccag tttctcgatg
    56401 cctctctgac gctggcgcgc cacccttcag aaacccagga gcgtatgatg ctgcgcttgc
    56461 tggcgtggct gaaatatgct gatgaacgtc tgcaatttac ccgtggtttg tgtgccgatg
    56521 atgagccgga agcgtggctg cgtaacgatc acctgggcat tgatttgtgg attgagctgg
    56581 ggctgccgga tgagcgacga attaagaaag cctgcaccca ggccgcagaa gtggcgctgt
    56641 ttacctataa tagtcgggcg gcgcaaatct ggtggcagca aaatcagagc aaatgtgtgc
    56701 agtttgccaa tctttccgtc tggtatctgg acgatgaaca actggcgaaa gtaagcgcct
    56761 ttgccgatcg taccatgacg ctgcaggcaa cgattcagga tggtgttatc tggttatcgg
    56821 atgataagaa taatctggaa gttaacttaa ccgcctggca acaaccttca tgattgtgat
    56881 ttcccgacat gttgctattc ccgatggtga gcttgagatc accgccattc gtgcgcaggg
    56941 cgctggcggg cagcatgtta ataagacctc aacggctatt catctgcgtt ttgacattcg
    57001 ggcgtccagc ctgccagagt attacaaaga gcgtctgctc gccgccagcc atcatttgat
    57061 cagcagtgat ggcgtgattg tcattaaggc acaggaatac cgcagtcagg aactgaaccg
    57121 cgaagccgct ctggcccggc tggtggcagt gattaaagat ttaacaaaag aacaaaaagc
    57181 ccgacgaccc acgcggccca cccgtgcatc gaaagagcgc aggctggcat cgaaagcaca
    57241 aaaatcaagc gtgaaggcga tgcgcggcaa agtacgcagc ggtcgggaat aaaaagaagg
    57301 aatggatggt gaaaaaagcg atagtgacag cgatggctgt aatcagcctc tttactctga
    57361 tgggatgtaa taatcgggcc gaagtcgata cgctttctcc ggcgcaggct gccgaactga
    57421 aaccgatgcc gcaaagttgg cgcggcgtgc taccgtgtgc cgattgcgaa ggaatcgaaa
    57481 tctctctgtt cctcgaaaaa gacggaacat gggtgatgaa tgagcgttat ctcggtgctc
    57541 gtgaagaacc ttcctccttc gcttcctacg gtacatgggc gcgaaccgct gacaagctgg
    57601 tattaaccga cagcaaaggt gaaaagtcat attatcgggc gaaaggcgat gcgctggaga
    57661 tgctcgatcg tgaaggcaat ccaattgaat cgcagttcaa ctatacgctg gaaccggcac
    57721 aatccagttt acctatgacg ccgatgaccc tgcgaggcat gtatttttat atggctgatg
    57781 cggcgacctt cactgattgc gcgaccggaa aacgtttcat ggtagcgaat aacgcagagc
    57841 tggagcgtag ctacctggct gcgcgcggtc acagtgaaaa accggtgtta ctgtcagtag
    57901 aaggtcactt tacgcttgag gctaatccgg atactggtgc gccaaccaaa gtattagcgc
    57961 ccgatacggc gggtaaattt tacccgaata aggattgcag tagtttgggg cagtaaacct
    58021 gcttaaggca gaaacaaacg caaaattgcc tgatgcgctg cgcttatcag gcctacagca
    58081 tataatgcaa cattttgaat tagcacgatt ttgtaggccg gataaggcgt ttacgccgca
    58141 tccggcattt tgtgcacgag gctggcttta tcactgagtc agcccaacaa agcgacccgc
    58201 cttgatataa atcccaggct tcaggtgccc aacatactgt aactgcgttt ctggcttaaa
    58261 cgcagagaca tcaccaatac gcatatgcat cagatcggca gggctaatcc agcccgattg
    58321 cgccagtgta agtggatgcc cggatttggc aaatgcatca gtgacaaatt ccgaacaaaa
    58381 ccacgacttt ttgtctcctt cgcacacact gcttaactgc gctttcgcca ggtcgctgac
    58441 gcactgttgg cgaaaatcct cggagaacgg attcagtgag cacatctggc gagtcaccat
    58501 aaagggaata aattcgacaa tgccgcgata gttataaccg ctatctttga ttttattggc
    58561 aaacgcggtg atatctgtgg cttgttgcgg ggtaagatcc gggactcgta agacgaaaag
    58621 cttatcacta tgcttcatcg cttttttaag ggaaacaatc tggacgccag cgcctgtcgc
    58681 ttctgcaacg ttattatcac ccagaaagat tgcaacgtga ctcacagagg aagtgctgaa
    58741 gacgcggatt ccaaatgagg ttaccccaag gcttgaggag aacagcaaat cgccgggttt
    58801 gagatcaggt gccgttattt cttttattga ttgttcggtg aaagagcttt gatgctggaa
    58861 tttgacagcc catgtttttg cctcggcatc tactgctgtg gctgatgggt ctggctggct
    58921 gatatcaacg gtacaagccg aaagtaaaag aaagctgggg agaagcaggc ggcagtacgc
    58981 ctttggttta tccattttat acaatccatg taaaaaaggg ccctgaaatt caggaccctt
    59041 tctggcatca gcctttaatc tgtttcacca gatattcgac gatgtcacca gtcttaatta
    59101 actgtttctc gccgttgcga cgatatttat attcgatatc gtcgttgtcg aggttacggt
    59161 cgcccagcac aatagtgtgc ggaataccga tcagttccat atcagcaaac atcacgcccg
    59221 gacgctcttt gcggtcatcc agcagcactt cgataccttg tgcacgcagt tcgctgtaca
    59281 gtttctcagc aagctcttgt acgcggaagg atttgtgcat gttcatcggc agaatcgcca
    59341 cctggaacgg cgcgatagcg tcaggccata cgatgccgcg ttcgtcgtag ttctgctcaa
    59401 tcgccgcagc taccacacgc gttaccccga taccgtagca acccatcgtc aggatttggt
    59461 tacggccatc ttcaccctgt acggaggctt tcagtgcttc ggagtacttg gtacccagct
    59521 ggaagatgtg accaacttcg ataccacgtt tgatcagcag cgtaccctgg ccatccgggc
    59581 ttggatcgcc agccaccacg ttacggatat ctgcaacttc cggggtagcg acatcgcgat
    59641 cccagttgat accgaagtag tgtttaccat cgatgttagc accagcagcg aaatcactca
    59701 tcgccgcaac ggtacggtca atcaccaccg gaatcggcat gtttaccgga cccagtgaac
    59761 ccggaccggc tttaaccaca gcacgaattt cttcttcggt cgcgaaagtc agcgggcttg
    59821 caacctgcgg cagtttttct gctttaactt cgttcagctc gtgatcaccg cgcaccagca
    59881 gcgcaaccag cgggaagcta ctgccttcaa ccgctttaac cagcagagtc ttaaccgttt
    59941 tctcaatcgg cagattgaac tgttcaacca gttccgcgat ggttttcgcg ttcggcgtat
    60001 caaccagcgt catttcctgg gtagcagcag cgcgcggttc tttcggcgcg atagcttctg
    60061 ccagttcaat gttcgctgca tagtcagagg tgtcggagaa gaccacatcg tcttcaccgc
    60121 tctgcgccag cacctggaat tcgtgagagg cgctgccgcc gatagaaccg gtgtcggcct
    60181 gtacggcgcg gaaatccagc cccatgcggc tgaagatttt gctgtaggcc gcatacattg
    60241 catcgtaggt ttcctgcagg gattcctgag aagtatggaa agagtaagca tctttcatca
    60301 ggaattcgcg ggaacgcatg acgccgaaac gcggacgcac ttcgtcgcgg aacttggtct
    60361 ggatctgata gaagttcagc ggcagctgtt tgtaagagct aagctcgtta cgaatcaggt
    60421 cagtgataac ttcttcatga gttgggccga gtacgaacgg acgctcgcca cggtcaacaa
    60481 aacgcagcag ttccggaccg tactgttccc aacgaccact ctcttgccac aaatcggctg
    60541 gctgaaccac cggcatcgac acctcgatcg caccggcgtt gttcatctct tcacgcacga
    60601 tgttttcgac ttttttcaga acgcgcacgc cggtcggcag ccaggtatat aacccggagg
    60661 ccagcttgcg gatcatcccg gcgcgcagca tcagctgatg gctgatcacc tcggcgtcgg
    60721 caggtgtctc cttgagagtg gagagcaggt attggctagt acgcatgttg ttacggttcc
    60781 agttggaagg tagaacaggc tcaaggcgag cctgggacaa aaaaagtgat ttagtttacc
    60841 agtgcaaaag aaatgtcaaa agagaagggc gtgaatttaa cgcggttcca gcgcaaagac
    60901 ttcaaaacct gcgtcggtga cgcgccagcg aacgttaaaa tcatgtagcc agacggcata
    60961 ggttttgccc gtttcctcac ctttacgata ggccgggcgc gggtcctgcg ccagtacttc
    61021 gcggataaac agcgttaact gcggataacg cttctccagc gtcaaaagct gcttttcgac
    61081 ctctgcggta aaactcaccg ccatctctgc agctggcgca ctttgcgcat agctggcact
    61141 ggcatcggga agcgattcgg caaaggggag atacggtttg atatccacta ccggcgtacc
    61201 atcgaccaga tccagactgc cgagcttcag aatcacgctg cctttatggc aaacaacctc
    61261 tttcagctct accagcgaca tgccaattgg gttagggcgg aaagtagagc gtgttgcgaa
    61321 aacccccatt ctggcgttac cgccgaggcg cggtggacgc acagtcggac gccagccgcc
    61381 ttccatcgtt tgatgaaaga cgaaaaggat ccataaatgg ctgaatgctt ccaggccgcg
    61441 aacggcgtcg gcctggttgt aggaagctat gagatgcagt tcgccgttgg cgctttttac
    61501 cagacctggc tggcgcggaa cggcgaattt ttctttatag ggcgagcgaa taacgcctat
    61561 ttgctcgaac tggaaactgc tcatttcgcc gtaatgttaa gcgcagagcc gatacataca
    61621 gcctgacgat agcagcccgg cgtaccgctg gtgacttcgc agctatgcag taatacagca
    61681 ttggctttca ttttagaggc attgatttgc atctgcttac gtgcggttgg aatgctcggc
    61741 ggagagtcct gattagaggc ctggcaagag tcgccactga cttcaccgag atcgcggaac
    61801 ggtttgccga ctaattcttc tgcattggta taaattcgga ccggcgtggc gcgcggcgct
    61861 ttcggttttg caggctccgc tttcggctgg ggtgcagtgc tttgaacggg ttcgacaggg
    61921 gatctgctta acatggaaca gccgcttagc atgagtgcta ctaaacagat cggtaaagca
    61981 cgcatagtat ttcctcaatg tatgatcaaa acgtcaatat tgaatcagga gcttgtaaaa
    62041 atgacaagac gggcaagcgc ccgtcctgaa tgatattaca aattgtggaa acagcctaaa
    62101 aattaccagc ctttaacagc gccgccgtta aacactttgt ttgctgcttc gtaaacttca
    62161 tcagactgat aagcctggac gaatttcttc acgttctcgg cgtctttgtt atcttcacgc
    62221 gttacgatca ggtttacgta tggggagtct ttatcttcga caaagatacc gtctttcgcc
    62281 ggagtcaggc caatctggct ggcataggtg gtattgataa ctgccagagc gatttgcgcg
    62341 tcgtccagag aacgcggcag ttgcggtgct tccagttcaa caattttcag attttttggg
    62401 ttctcaacaa catcaagaac ggtcggcagc aggccaacgc catctttcag tttgatcaag
    62461 cccacttttt gcagcagcag cagtgaacga ccaagattag ttggatcgtt tggcacggca
    62521 acctgcgaac catcctgcag ttcatccagt gatttgattt tcttggagta accagcaatc
    62581 ggataaacaa aagtgttgcc tactgcgacc agtttgtagc cacgatcttt cagttgctga
    62641 tcaaggtacg gtttatgctg gaaggcgttg gcgtcgatat cgcctttgct caatgcttcg
    62701 tttggcagaa catagtcgtt gaaggttacc agctcaacgt ccaggccata tttgtctttc
    62761 gcaactttct gcgcgacttc tgctacctgc tgttcggcac caacaatcac gccgacttta
    62821 atgtggtttg gatctttttc atcctgaccg cagcctacca gtgccagtga tccaatcagg
    62881 gctcccactg ccgcaaaggt tttgaatttg aacgccatac cttattcctt cttcaattat
    62941 ttatgttgtg ttgaacgtta cttgcgagtg acagcccgga cgatgcggtc gcctgcaaac
    63001 tgaattaaat aaaccagaat gaccagcaat accagtaccg tattcatcac cgtcgcgttg
    63061 tagccgatgt agccatactg atagccaatc tgacctaaac caccggcacc gactgcacca
    63121 cccatcgcgg aataaccgac cagggtaatc agggtgatag ttgccgcatt caccagaccc
    63181 ggcaacgctt ccggtaacag cactttacag acgatctgca ttggcgtggc acccattgcg
    63241 cgggaagctt caattaaccc ggttgggatc tccagcagag cgttctcgac catacgggca
    63301 ataaacggtg ctgcaccaac ggttaacgga acaattgctg cctgcaaacc aatcgatgta
    63361 ccgacaataa cgcgggtaaa cggaatcatc cataccagca agataatgaa cgggatggaa
    63421 cggaaaatgt tcacaatcgc agaaacggta cgatacagct tcgcgttagc aataatttgc
    63481 cccggacgcg tgacataaag cagaacgcca accggcagac caatcacaaa cccaaaaaat
    63541 ccggatacga aggtcattgc cagcgtttcc catacgccac gaaccagcag ccacatcatc
    63601 ggctcagaca taacccagta cctctacttt tacatggtgt tcctgcagcc aggcaatggc
    63661 ggcttgcgta tcttgttgtg tgccgtgcat ttcagtcagc atgatgccga atttaacgcc
    63721 accggcgtag tccatctgcg cgctaataat gttgttgttg acgttgaaac gacgcgcggt
    63781 ttcagaaagc agtggggcat cgaccgattg accggtaaac tccagacgca gcatcggcac
    63841 gcagtcagta aatggctccg tttgcagacg ttcctggtaa tcttccggga tatccagatg
    63901 cagggtcgac tgaataaact tctgcgccag cggcgttttc ggatgcgaga acacttcact
    63961 taccgtgtcc tgctcgatca gttcgccatt gctgatgacc gccacgcaat cacaaatacg
    64021 cttcacaacg tccatttcgt gggtgatcaa cagaatcgtc aaacccagac ggcggttaat
    64081 gtctttcagc agttcgagaa tagaacgtgt cgttgccggg tccagcgcgc tggtggcttc
    64141 atcacacagc aatactttgg gattgctggc taatgcacgg gcaatcgcca cacgttgttt
    64201 ctgcccaccg gaaagattcg acgggtagct atcatgctta tcgccaagac caaccaatga
    64261 cagcaattcc gtcacgcgac gtttgatctc gtctttcggt gtgttgtcca gctccagcgg
    64321 cagagccacg ttgccaaaaa cagtacgcga agagagcagg ttaaaatgct ggaaaatcat
    64381 accaatctgg cggcgagctt tggtcaactc ggattctgac agcgtggtca attcctggcc
    64441 atcgaccagc acgctaccct cggttgggcg ctccagcaag tttacacaac gtataagcgt
    64501 actcttaccc gcgcctgagg caccgataac gccataaatt tgtccagctg gcacatgcag
    64561 gctgacgttg ttcaacgcct ggatggtgcg ggtgccctgg tggaacactt tggtgatatt
    64621 cgaaagtttt atcattgatt atttattatc gtcattaagt tagtcgtagc atctcgaatg
    64681 cctgaaacgg gcaacgccgt caatgaaatg gatgttaagg catccagacg tctaaatcaa
    64741 tcaggtttat gcgaagagca ctttcttgca ggtcgaaaca tgcgatacta gcgtcccatg
    64801 ccttattaag gagctataaa aggtggcgaa gagcgtaccc gcaatttttc ttgaccgtga
    64861 tggcaccatt aatgtcgatc acggctatgt ccatgagatc gacaactttg aatttatcga
    64921 cggtgttatt gacgccatgc gcgagctaaa aaaaatgggc tttgcgctgg tggtagtaac
    64981 caaccagtct ggcattgctc gcggtaaatt taccgaagca cagtttgaaa cgctgaccga
    65041 gtggatggac tggtcgctgg cggaccgaga tgtcgatctg gatggtatct attattgccc
    65101 gcatcatccg caggggagtg ttgaagagtt tcgccaggtc tgcgattgcc gcaaaccaca
    65161 tccggggatg cttttgtcag cacgcgatta tttgcatatt gatatggccg cttcttatat
    65221 ggtgggcgat aaattagaag atatgcaggc agcggttgcg gcgaacgtgg gaacaaaagt
    65281 gctggtgcgt acgggtaaac ctattacacc tgaagcagaa aacgcggcag attgggtgtt
    65341 aaatagcctg gcagacctgc cgcaagcgat aaaaaagcag caaaaaccgg cgcaatgatt
    65401 aaaagatgaa cggttgaaat aaaaatgcat ttttccgctt gtcttcctga gccgactccc
    65461 tataatgcgc ctccatcgac acggcggatg tgaatcactt cacacaaaca gccggttcgg
    65521 ttgaagagaa aaaatcctga aattcagggt tgactctgaa ataggaaagt gtgatatacg
    65581 ccacctcgcg acagtgagct gaaagctgcg tcgcaactgc tctttaacaa tttatcagac
    65641 aatctgtgtg ggcactcgaa gatacggatt cttaacgtcg caagacgata aatgaatacc
    65701 aagtctcaag agtgaacacg taattcatta cgaagtttaa ttctttgagc atcaaacttt
    65761 taaattgaag agtttgatca tggctcagat tgaacgctgg cggcaggcct aacacatgca
    65821 agtcgaacgg taacaggaag cgcttgctgc ttcgctgacg agtggcggac gggtgagtaa
    65881 tgtctgggaa actgcctgat ggagggggat aactactgga aacggtagct aataccgcat
    65941 aacgtcgcaa gaccaaagag ggggaccttc gggcctcttg ccatcggatg tgcccagatg
    66001 ggattagctt gttggtgggg taacggctca cc
//
LOCUS       DAJTRC010000011        64184 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.011, whole genome shotgun sequence.
ACCESSION   DAJTRC010000011 DAJTRC010000000
VERSION     DAJTRC010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 64184)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 64184)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..64184
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.011"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            121..564
                     /locus_tag="OO357_000761"
     CDS             121..564
                     /locus_tag="OO357_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004997737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5885810.1"
                     /translation="MDLALVTGQRKGDLAAMRWEDIRDSRLYVEQQKTGAKIRISLPT
                     TISRLNLTLADVLDNLKKINGKNEKLLGGKTARTIAAQFRIARDTSGLKWEGDPPPFH
                     EIRSLSGRLHSAEKGSDFTQALLGHRSSSMTDKYRDGRGREWKDI"
     gene            800..1618
                     /gene="ybhA"
                     /locus_tag="OO357_000762"
     CDS             800..1618
                     /gene="ybhA"
                     /locus_tag="OO357_000762"
                     /EC_number="3.1.3.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706475.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyridoxal
                     phosphate/fructose-1,6-bisphosphate phosphatase"
                     /protein_id="HCR5885811.1"
                     /translation="MTTRVIALDLDGTLLTPKKTLLPSSIEALARAREAGYQLIIVTG
                     RHHVAIHPFYQALALDTPAICCNGTYLYDYHAKTVLEADPMPVNKALQLIEMLNEHHI
                     HGLMYVDDAMVYEHPTGHVIRTSNWAQTLPPEQRPTFTQVASLAETTQQVNAVWKFAL
                     THDDLPQLQHFGKHVEHELGLECEWSWHDQVDIAHGGNSKGKRLTKWVEAQGWSMENV
                     VAFGDNFNDISMLEAAGTGVAMGNADDAVKARANIVIGDNTTDSIAQFIYSHLI"
     gene            complement(1619..2677)
                     /gene="modC"
                     /locus_tag="OO357_000763"
     CDS             complement(1619..2677)
                     /gene="modC"
                     /locus_tag="OO357_000763"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein
                     ModC"
                     /protein_id="HCR5885812.1"
                     /translation="MLELNFSQTLGNHCLTINETLPANGITAIFGVSGAGKTSLINAI
                     SGLTRPQKGRIVLNGRVLNDAEKGICLTPEKRRVGYVFQDARLFPHYKVRGNLRYGMS
                     KSMVDQFDKLVALLGIEPLLDRLPGSLSGGEKQRVAIGRALLTAPELLLLDEPLASLD
                     IPRKRELLPYLQRLTREINIPMLYVSHSLDEILHLADRVMVLENGQVKAFGALEEVWG
                     SSVMNPWLPKEQQSSILKVTVLEHHPHYAMTALALGDQHLWVNKLGEPLQAALRIRIQ
                     ASDVSLVLQPPQQTSIRNVLRAKVVNSYDDNGQVEVELEVGGKTLWARISPWARDELA
                     IKPGLWLYAQIKSVSITA"
     gene            complement(2680..3369)
                     /gene="modB"
                     /locus_tag="OO357_000764"
     CDS             complement(2680..3369)
                     /gene="modB"
                     /locus_tag="OO357_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HCR5885813.1"
                     /translation="MILTDPEWQAVLLSLKVSSLAVLFSLPFGIFFAWLLVRCTFPGK
                     ALLDSVLHLPLVLPPVVVGYLLLVSMGRRGFIGERLYDWFGITFAFSWRGAVLAAAVM
                     SFPLMVRAIRLALEGVDVKLEQAARTLGAGRWRVFFTITLPLTLPGIIVGTVLAFARS
                     LGEFGATITFVSNIPSETRTIPSAMYTLIQTPGGESGAARLCIISIALAMISLLISEW
                     LARISRERAGR"
     gene            complement(3369..4142)
                     /gene="modA"
                     /locus_tag="OO357_000765"
     CDS             complement(3369..4142)
                     /gene="modA"
                     /locus_tag="OO357_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5885814.1"
                     /translation="MARKWLNLFAGAALSFAVAGNALADEGKITVFAAASLTNAMQDI
                     ATQYKKEKGVDVVSSFASSSTLARQIEAGAPADLFISADQKWMDYAVDKKAIDTATRQ
                     TLLGNSLVVVAPKASEQKDFTIDSKTNWTSLLNGGRLAVGDPEHVPAGIYAKEALQKL
                     GAWDTLSPKLAPAEDVRGALALVERNEAPLGIVYGSDAVASKGVKVVATFPEDSHKKV
                     EYPVAVVEGHNNATVKAFYDYLKGPQAAEIFKRYGFTTK"
     gene            complement(4309..4458)
                     /gene="acrZ"
                     /locus_tag="OO357_000766"
     CDS             complement(4309..4458)
                     /gene="acrZ"
                     /locus_tag="OO357_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump accessory protein AcrZ"
                     /protein_id="HCR5885815.1"
                     /translation="MLELLKSLVFAVIMVPVVMAIILGLIYGLGEVFNIFSGVGKKDQ
                     PGQNH"
     gene            4587..5375
                     /gene="modE"
                     /locus_tag="OO357_000767"
     CDS             4587..5375
                     /gene="modE"
                     /locus_tag="OO357_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum-dependent transcriptional regulator"
                     /protein_id="HCR5885816.1"
                     /translation="MQAEILLTLKLQQKLFADPRRISLLKHIALSGSISQGAKDAGIS
                     YKSAWDAINEMNQLSEHILVERATGGKGGGGAVLTRYGQRLIQLYDLLAQIQQKAFDV
                     LSDDDALPLNSLLAAISRFSLQTSARNQWFGTITARDHDDVQQHVDVLLADGKTRLKV
                     AITAQSGARLGLDEGKEVLILLKAPWVGITQDEAVAQNADNQLPGIISHIERGAEQCE
                     VLMALPDGQTLCATVPVNEATSLQQGQNVTAYFNADSVIIATLC"
     gene            5443..6915
                     /gene="modF"
                     /locus_tag="OO357_000768"
     CDS             5443..6915
                     /gene="modF"
                     /locus_tag="OO357_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter ATP-binding protein
                     ModF"
                     /protein_id="HCR5885817.1"
                     /translation="MSSLQILQGTFRLSDTKTLQLPQLTLNAGDSWAFVGSNGSGKSA
                     LARALAGELPLLKGERQSQFSHITRLSFEQLQKLVSDEWQRNNTDMLGPSEDYTGRTT
                     AEIIQDEVKDAPRCMQLAQQFGITALLDRRFKYLSTGETRKTLLCQALMSEPDLLILD
                     EPFDGLDVASRQQLAERLASLHQSGITLVLVLNRFDKIPEFVQFAGVLADCTLAETGA
                     KEELLQQALVAQLAHSEQLEGVQLPEPDEPSARHALPANESRIVLNNGVVSYNDRPIL
                     NNLSWQVNPGEHWQIVGPNGAGKSTLLSLITGDHPQGYSNDLTLFGRRRGSSETIWDI
                     KKHIGYVSSSLHLDYRVSTTVRNVILSGYFDSIGIYQVVSDRQQKLVQQWLDILGIDK
                     HTADAPFHSLSWGQQRLALIVRALVKHPTLLILDEPLQGLDPLNRQLIRRFVDVLISE
                     GETQLLFVSHHAEDAPACITHRLEFVPDGGLYRYVLTKIY"
     gene            7176..8192
                     /gene="galE"
                     /locus_tag="OO357_000769"
     CDS             7176..8192
                     /gene="galE"
                     /locus_tag="OO357_000769"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706482.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HCR5885818.1"
                     /translation="MRVLVTGGSGYIGSHTCVQLLQNGHDVIILDNLCNSKRSVLPVI
                     ERLGGKHPTFVEGDIRNEALMTEILHDHAIDTVIHFAGLKAVAESVQKPLEYYDNNVN
                     GTLRLISAMRAANVKNFIFSSSATVYGDQPQIPYVESFPTGTPQSPYGKSKLMVEQIL
                     TDLQKAQPDWSIALLRYFNPVGAHPSGDMGEDPQGIPNNLMPYIAQVAVGRRDSLAIF
                     GNDYPTEDGTGVRDYIHVMDLADGHVVAMEKLANKPGVHIYNLGAGVGSSVLDVVNAF
                     SKACGKPVNYHFAPRREGDLPAYWADASKADRELNWRVTRTLDEMAQDTWHWQSRHPQ
                     GYPD"
     gene            8202..9248
                     /gene="galT"
                     /locus_tag="OO357_000770"
     CDS             8202..9248
                     /gene="galT"
                     /locus_tag="OO357_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-phosphate uridylyltransferase"
                     /protein_id="HCR5885819.1"
                     /translation="MTQFNPVDHPHRRYNPLTGQWILVSPHRAKRPWQGAQETPAKQV
                     LPAHDPDCFLCAGNVRVTGDKNPDYTGTYVFTNDFAALMSDTPDAPESNDPLMRCQSA
                     RGTSRVICFSPDHSKTLPELSVAALTEIVKTWQEQTAELGKTYPWVQVFENKGAAMGC
                     SNPHPHGQIWANSFLPNEAECEDRLQKEYFAEQKSPMLVDYVQRELADGSRTVVETEH
                     WLAVVPYWAAWPFETLLLPKAHVLRITDLTDAQRSDLALALKKLTSRYDNLFQCSFPY
                     SMGWHGAPFNGEENQHWQLHAHFYPPLLRSATVRKFMVGYEMLAETQRDLTAEQAAER
                     LRAVSDIHFRESGV"
     gene            9252..10400
                     /gene="galK"
                     /locus_tag="OO357_000771"
     CDS             9252..10400
                     /gene="galK"
                     /locus_tag="OO357_000771"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001713229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HCR5885820.1"
                     /translation="MSLKEKTQSLFANAFGYPATHTIQAPGRVNLIGEHTDYNDGFVL
                     PCAIDYQTVISCAPRDDRKVRVMAADYENQLDEFSLNAPIVAHENYQWANYVRGVVKH
                     LQLRNNSFGGVDMVISGNVPQGAGLSSSASLEVAVGTVLQQLYHLPLDGAQIALNGQE
                     AENQFVGCNCGIMDQLISALGKKDHALLIDCRSLGTKAVSMPKGVAVVIINSNFKRTL
                     VGSEYNTRREQCETGARFFQQPALRDVTIEEFNAVAHELDPIVAKRVRHILTENARTV
                     EAASALEQGDLKRMGELMAESHASMRDDFEITVPQIDTLVEIVKAVIGDKGGVRMTGG
                     GFGGCIVALIPEELVPAVQQAVAEQYEAKTGIKETFYVCKPSQGAGQC"
     gene            10394..11434
                     /gene="galM"
                     /locus_tag="OO357_000772"
     CDS             10394..11434
                     /gene="galM"
                     /locus_tag="OO357_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-1-epimerase"
                     /protein_id="HCR5885821.1"
                     /translation="MLNETPALAPDGQPYRLLTLRNNAGMVVTLMDWGATLLSARIPL
                     SDGSVREALLGCASPECYQDQAAFLGASIGRYANRIANSRYTFDGETVTLSPSQGVNQ
                     LHGGPEGFDKRRWQIVNQNDRQVLFALSSDDGDQGFPGNLGATVQYRLTDDNRISITY
                     RATVDKPCPVNMTNHVYFNLDGEQSDVRNHKLQILADEYLPVDEGGIPHDGLKSVAGT
                     SFDFRSAKIIASEFLADDDQRKVKGYDHAFLLQAKGDGKKVAAHVWSADEKLQLKVYT
                     TAPALQFYSGNFLGGTPSRGTEPYADWQGLALESEFLPDSPNHPEWPQPDCFLRPGEE
                     YSSLTEYQFIAE"
     gene            11637..12389
                     /gene="gpmA"
                     /locus_tag="OO357_000773"
     CDS             11637..12389
                     /gene="gpmA"
                     /locus_tag="OO357_000773"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000301556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HCR5885822.1"
                     /translation="MAVTKLVLVRHGESQWNKENRFTGWYDVDLSEKGVSEAKAAGKL
                     LKEEGYSFDFAYTSVLKRAIHTLWNVLDELDQAWLPVEKSWKLNERHYGALQGLNKAE
                     TAEKYGDEQVKQWRRGFAVTPPELTKDDERYPGHDPRYAKLSEKELPLTESLALTIDR
                     VIPYWNETILPRMKSGERVIIAAHGNSLRALVKYLDNMSEEEILELNIPTGVPLVYEF
                     DENFKPLKRYYLGNADEIAAKAAAVANQGKAK"
     gene            complement(12556..13608)
                     /gene="aroG"
                     /locus_tag="OO357_000774"
     CDS             complement(12556..13608)
                     /gene="aroG"
                     /locus_tag="OO357_000774"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001109196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroG"
                     /protein_id="HCR5885823.1"
                     /translation="MNYQNDDLRIKEIKELLPPVALLEKFPATENAANTVAHARKAIH
                     KILKGNDDRLLVVIGPCSIHDPVAAKEYATRLLALREELKDELEIVMRVYFEKPRTTV
                     GWKGLINDPHMDNSFQINDGLRIARKLLLDINDSGLPAAGEFLDMITPQYLADLMSWG
                     AIGARTTESQVHRELASGLSCPVGFKNGTDGTIKVAIDAINAAGAPHCFLSVTKWGHS
                     AIVNTSGNGDCHIILRGGKEPNYSAKHVAEVKEGLNKAGLPAQVMIDFSHANSSKQFK
                     KQMDVCADVCQQIAGGEKAIIGVMVESHLVEGNQSLDSGEPLAYGKSITDACIGWEDT
                     DALLRQLVNAVKARRG"
     gene            13924..14304
                     /locus_tag="OO357_000775"
     CDS             13924..14304
                     /locus_tag="OO357_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgS-like family protein"
                     /protein_id="HCR5885824.1"
                     /translation="MKMTKLATLFLTATLSLASGAALAADSGAQTNNGQANAAADAGQ
                     VAPDARENVAPNNVDNNGVNTGSGGTMLHSDGSSMNNDGMTKDEEHKNTMCKDGRCPD
                     INKKVQTGDGINNDVDTKTDGTTQ"
     gene            14418..15359
                     /gene="zitB"
                     /locus_tag="OO357_000776"
     CDS             14418..15359
                     /gene="zitB"
                     /locus_tag="OO357_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family zinc transporter ZitB"
                     /protein_id="HCR5885825.1"
                     /translation="MAHSHSHTSSHLPEDNNARRLLYAFGVTAGFMLVEVVGGFLSGS
                     LALLADAGHMLTDTAALLFALLAVQFSRRPPTIRHTFGWLRLTTLAAFVNAIALVVIT
                     ILIVWEAIERFRTPRPVEGGMMMAIAVAGLLANILSFWLLHHGSEEKNLNVRAAALHV
                     LGDLLGSVGAIIAALIIIWTGWTPADPILSILVSLLVLRSAWRLLKDSVNELLEGAPV
                     SLDIAELKRRMCREIPEVRNVHHVHVWMVGEKPVMTLHVQVIPPHDHDALLDQIQHYL
                     MDHYQIEHTTIQMEYQPCHRPDCHLNEGVSGHSHHHH"
     gene            complement(15356..16075)
                     /gene="pnuC"
                     /locus_tag="OO357_000777"
     CDS             complement(15356..16075)
                     /gene="pnuC"
                     /locus_tag="OO357_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide riboside transporter PnuC"
                     /protein_id="HCR5885826.1"
                     /translation="MDFFSVQNILVHIPIGAGGYDLSWIEAVGTIAGLLCIGLASLEK
                     ISNYFFGLINVTLFGIIFFQIQLYASLLLQVFFFAANIYGWYAWSRQTSQNEAELKIR
                     WLPLPKALSWLAVCVVSIGLMTVFINPVFAFLTRVAVMIMQALGLQVVMPELQPDAFP
                     FWDSCMMVLSIVAMILMTRKYVENWLLWVIINVISVVIFALQGVYAMSLEYIILTFIA
                     LNGSRMWINSARERGSRALSH"
     gene            complement(16113..17156)
                     /gene="nadA"
                     /locus_tag="OO357_000778"
     CDS             complement(16113..17156)
                     /gene="nadA"
                     /locus_tag="OO357_000778"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HCR5885827.1"
                     /translation="MSVMFDPDTAIYPFPPKPTPLSIDEKAYYREKIKRLLKERNAVM
                     VAHYYTDPEIQQLAEETGGCISDSLEMARFGAKHPASTLLVAGVRFMGETAKILSPEK
                     TILMPTLQVECSLDLGCPVEEFNAFYDAHPDRTVVVYANTSAAVKARADWVVTSSIAV
                     ELIDHLDSLGEKIIWAPDKHLGRYVQKQTGGDILCWQGACIVHDEFKTQALTRLQEEY
                     PDAAILVHPESPQAIVDMADAVGSTSQLIAAAKALPHQRLIVATDRGIFYKMQQAVPD
                     KELLEAPTAGEGATCRSCAHCPWMAMNDLQAIAEALEQEGSNHEVHVDERLRERALVP
                     LNRMLDFAATLRG"
     gene            complement(17405..17480)
                     /locus_tag="OO357_000779"
     tRNA            complement(17405..17480)
                     /locus_tag="OO357_000779"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17445..17447),aa:Lys,seq:ttt)
     gene            complement(17484..17559)
                     /locus_tag="OO357_000780"
     tRNA            complement(17484..17559)
                     /locus_tag="OO357_000780"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17524..17526),aa:Val,seq:tac)
     gene            complement(17611..17686)
                     /locus_tag="OO357_000781"
     tRNA            complement(17611..17686)
                     /locus_tag="OO357_000781"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17651..17653),aa:Lys,seq:ttt)
     gene            complement(17689..17764)
                     /locus_tag="OO357_000782"
     tRNA            complement(17689..17764)
                     /locus_tag="OO357_000782"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17729..17731),aa:Val,seq:tac)
     gene            complement(17900..17975)
                     /locus_tag="OO357_000783"
     tRNA            complement(17900..17975)
                     /locus_tag="OO357_000783"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17940..17942),aa:Lys,seq:ttt)
     gene            complement(18140..18931)
                     /gene="cpoB"
                     /locus_tag="OO357_000784"
     CDS             complement(18140..18931)
                     /gene="cpoB"
                     /locus_tag="OO357_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CpoB"
                     /protein_id="HCR5885828.1"
                     /translation="MSSNFRHQLLSLSLLVGIAAPWAAFAQAPISSVSSGSVEDRVTQ
                     LERISNAHSQLLTQLQQQLSDNQSDIDSLRGQIQENQYQLNQVVERQKQILLQIDSLS
                     SGGAAAQSTSSDQSGATASTTPTADAGTANAGAPVKSGDANTDYNAAIALVQDKSRQD
                     DAMVAFQNFIKNYPDSTYLPNANYWLGQLNYNKGKKDDAAYYFASVVKNYPKSPKAAD
                     AMFKVGVIMQDKGDTAKAKAVYQQVISKYPGTDGAKQAQKRLNAM"
     gene            complement(18941..19462)
                     /gene="pal"
                     /locus_tag="OO357_000785"
     CDS             complement(18941..19462)
                     /gene="pal"
                     /locus_tag="OO357_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HCR5885829.1"
                     /translation="MQLNKVLKGLMIALPVMAIAACSSNKNASNDGSEGMLGAGTGMD
                     ANGGNGNMSSEEQARLQMQQLQQNNIVYFDLDKYDIRSDFAQMLDAHANFLRSNPSYK
                     VTVEGHADERGTPEYNISLGERRANAVKMYLQGKGVSADQISIVSYGKEKPAVLGHDE
                     AAYSKNRRAVLVY"
     gene            complement(19497..20789)
                     /gene="tolB"
                     /locus_tag="OO357_000786"
     CDS             complement(19497..20789)
                     /gene="tolB"
                     /locus_tag="OO357_000786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001646851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolB"
                     /protein_id="HCR5885830.1"
                     /translation="MKQALRVAFGFLILWASVLHAEVRIVIDSGVDSGRPIGVVPFQW
                     AGPGAAPEDIGGIVAADLRNSGKFNPLDRARLPQQPGSAQEVQPAAWSALGIDAVVVG
                     QVTPNPDGSYNVAYQLVDTGGAPGTVLAQNSYKVNKQWLRYAGHTASDEVFEKLTGIK
                     GAFRTRIAYVVQTNGGQFPYELRVSDYDGYNQFVVHRSPQPLMSPAWSPDGSKLAYVT
                     FESGRSALVIQTLANGAVRQVASFPRHNGAPAFSPDGSKLAFALSKTGSLNLYVMDLA
                     SGQIRQVTDGRSNNTEPTWFPDSQNLAFTSDQAGRPQVYKVNINGGAPQRITWEGSQN
                     QDADVSSDGKFMVMVSSNGGQQHIAKQDLATGGVQVLSSTFLDETPSLAPNGTMVIYS
                     SSQGMGSVLNLVSTDGRFKARLPATDGQVKFPAWSPYL"
     gene            complement(20922..22163)
                     /gene="tolA"
                     /locus_tag="OO357_000787"
     CDS             complement(20922..22163)
                     /gene="tolA"
                     /locus_tag="OO357_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein TolA"
                     /protein_id="HCR5885831.1"
                     /translation="MSKATEQNDKLKRAIIISAVLHVILFAALIWSSFDENIEASAGG
                     GGGSSIDAVMVDSGAVVEQYKRMQSQESSAKRSDEQRKMKEQQAAEELREKQAAEQER
                     LKQLEKERLAAQEQKKQAEEAAKQAELKQKQAEVAAAKAAADAKAAEEAAKKAAADAK
                     KKAEAEAAKAAAEAQKKAEVAAAALKKKAEAAEAAAAEARKKAATEAAEKAKAEAEKK
                     AAAEKAAADKKAAAEKAAADKKAAEKAAADKAAADKKAAAEKAAADKKAAAAKAAAEK
                     AAAAKAAAEADDIFGELSSGKNAPKTGGGAKGNNASPAGSGNTKNNGASGADINNYAG
                     QIKSAIESKFYDASSYAGKTCTLRIKLAPDGMLLDIKPEGGDPALCQAALAAAKLAKI
                     PKPPSQAVYEVFKNAPLDFKP"
     gene            complement(22228..22656)
                     /gene="tolR"
                     /locus_tag="OO357_000788"
     CDS             complement(22228..22656)
                     /gene="tolR"
                     /locus_tag="OO357_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin uptake protein TolR"
                     /protein_id="HCR5885832.1"
                     /translation="MARARGRGRRDLKSEINIVPLLDVLLVLLLIFMATAPIITQSVE
                     VDLPDATESQAVSSNDNPPVIVEVSGIGQYTVVVEKDRLERLPPEQVVAEVSSRFKAN
                     PKTVFLIGGAKDVPYDEIIKALNLLHSAGVKSVGLMTQPI"
     gene            complement(22660..23352)
                     /gene="tolQ"
                     /locus_tag="OO357_000789"
     CDS             complement(22660..23352)
                     /gene="tolQ"
                     /locus_tag="OO357_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tol-Pal system protein TolQ"
                     /protein_id="HCR5885833.1"
                     /translation="MTDMNILDLFLKASLLVKLIMLILIGFSIASWAIIIQRTRILNA
                     AAREAEAFEDKFWSGIELSRLYQESQGKRDNLTGSEQIFYSGFKEFVRLHRANSHAPE
                     AVVEGASRAMRISMNRELENLETHIPFLGTVGSISPYIGLFGTVWGIMHAFIALGAVK
                     QATLQMVAPGIAEALIATAIGLFAAIPAVMAYNRLNQRVNKLELNYDNFMEEFTAILH
                     RQAFTVSESNKG"
     gene            complement(23349..23753)
                     /gene="ybgC"
                     /locus_tag="OO357_000790"
     CDS             complement(23349..23753)
                     /gene="ybgC"
                     /locus_tag="OO357_000790"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HCR5885834.1"
                     /translation="MNTTLFRWPVRVYYEDTDAGGVVYHASYVAFYERARTEMLRHHH
                     FSQQALMAERVAFVVRKMTVEYYAPARLDDMLEIQTEITSMRGTSLVFTQRIVNAENT
                     LLNEAEVLVVCVDPLKMKPRALPKSIVAEFKQ"
     gene            complement(23903..24196)
                     /gene="ybgE"
                     /locus_tag="OO357_000791"
     CDS             complement(23903..24196)
                     /gene="ybgE"
                     /locus_tag="OO357_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyd operon protein YbgE"
                     /protein_id="HCR5885835.1"
                     /translation="MSKIIATLYAVMDKRPLRALSFVMALLLAGCMFWDPSRFAAKTS
                     ELEIWHGLLLMWAVCAGVIHGVGFRPQKVLWQGIFCPLLADIVLIVGLIFFFF"
     gene            complement(24196..24309)
                     /gene="cydX"
                     /locus_tag="OO357_000792"
     CDS             complement(24196..24309)
                     /gene="cydX"
                     /locus_tag="OO357_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-I oxidase subunit CydX"
                     /protein_id="HCR5885836.1"
                     /translation="MWYFAWILGTLLACSFGVITALALEHVESGKAGQEDI"
     gene            complement(24324..25463)
                     /gene="cydB"
                     /locus_tag="OO357_000793"
     CDS             complement(24324..25463)
                     /gene="cydB"
                     /locus_tag="OO357_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000568269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5885837.1"
                     /translation="MIDYEVLRFIWWLLVGVLLIGFAVTDGFDMGVGMLTRFLGRNDT
                     ERRIMINSIAPHWDGNQVWLITAGGALFAAWPMVYAAAFSGFYVAMILVLASLFFRPV
                     GFDYRSKIEETRWRNMWDWGIFIGSFVPPLVIGVAFGNLLQGVPFNVDEYLRLYYTGN
                     FFQLLNPFGLLAGVVSVGMIITQGATYLQMRTVGELHLRTRATAQVAALVTLVCFALA
                     GVWVMYGIDGYVVKSTMDHYAASNPLNKEVVREAGAWLVNFNNTPILWAIPALGVVLL
                     LLTILTARMDKAAWAFVFSSLTLACIILTAGIAMFPFVMPSSTMMNASLTMWDATSSQ
                     LTLNVMTWVAVVLVPIILLYTAWCYWKMFGRITKEDIERNTHSLY"
     gene            complement(25479..27047)
                     /gene="cydA"
                     /locus_tag="OO357_000794"
     CDS             complement(25479..27047)
                     /gene="cydA"
                     /locus_tag="OO357_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415261.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HCR5885838.1"
                     /translation="MLDIVELSRLQFALTAMYHFLFVPLTLGMAFLLAIMETVYVLSG
                     KQIYKDMTKFWGKLFGINFALGVATGLTMEFQFGTNWSYYSHYVGDIFGAPLAIEGLM
                     AFFLESTFVGLFFFGWDRLGKVQHMCVTWLVALGSNLSALWILVANGWMQNPIASDFN
                     FETMRMEMVSFSELVLNPVAQVKFVHTVASGYVTGAMFILGISAWYMLKGRDFAFAKR
                     SFAIAASFGMAAVLSVIVLGDESGYEMGDVQKTKLAAIEAEWETQPAPAAFTLFGIPD
                     QEEETNKFAIQIPYALGIIATRSVDTPVIGLKELMVQHEERIRNGMKAYSLLEQLRSG
                     STDQTVRDQFNSMKKDLGYGLLLKRYTPNVADATEAQIQQATKDSIPRVAPLYFAFRI
                     MVACGFLLLAIIALSFWSVIRNRIGEKKWLLRAALYGIPLPWIAVEAGWFVAEYGRQP
                     WAIGEVLPTAVANSSLTAGDLIFSMVLICGLYTLFLVAELFLMFKFARLGPSSLKTGR
                     YHFEQSSTTTQPAR"
     gene            complement(27896..30529)
                     /gene="mngB"
                     /locus_tag="OO357_000795"
     CDS             complement(27896..30529)
                     /gene="mngB"
                     /locus_tag="OO357_000795"
                     /EC_number="3.2.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosylglycerate hydrolase"
                     /protein_id="HCR5885839.1"
                     /translation="MKAVSRVHITPHMHWDREWYFTTEESRILLVNNMEEILCRLEQD
                     NEYKYYVLDGQTAILEDYFAVKPENKDRLKKLVEAGKLIIGPWYTQTDTTIVSAESIV
                     RNLMYGMRDCLAFGEPMKIGYLPDSFGMSGQLPHIYNGFGITCTMFWRGCSERHGTDK
                     TEFLWQSSDGSEVTAQVMPLGYAIGKYLPADENGLRKRLDSYFDVLEKASVTKEILLP
                     NGHDQMPLQQNIFEVMEKLREIYPQRKFVMSRFEEVFEKIEAQRDNLATLKGEFIDGK
                     YMRVHRTIGSTRMDIKVAHARIENKIVNLLEPLATLARTLGFGYHHGLLEKMWKEILK
                     NHAHDSIGCCCRDKVHREIVARFELAEDMADNLLRFYMRKIADNMPQSDADKLVLFNL
                     MPWPREEVINTTVRLRASQFNLRDDRGQPVPYFIRHAREIDPGLIDRQIVHYGNYDPF
                     MEFDIQINQIVPSMGYRTLYIEANQPGNVIAAKSDAEGILENAFWQIALNEDGTLQLV
                     DKDSGVRYDRVLQIEESSDDGDEYDYSPAKEEWVITSANAKPQCEIIHEAWQSRAVIR
                     YEMAVPRNLQQRSARQSTGRVDVELVVTLSHNNRRIDVDINLDNQADDHRLRVLIPTS
                     FNTDSVLADTQFGSLTRPVNDSAMNNWQQESWKEAPVPVWNMLNYAALQEGRNGMAVF
                     SEGLREFEVIGEEKKTFAITLLRGVGLLGKEDLLLRPRRPSGIKMPVPDSQLRGLLSC
                     RLSLLSYTGTPTAAGVAQQARAWLTPVQCYNKIPWDAMKLNKAGFNVPESYSLLKMPP
                     VGCLISALKKAEDRQEVILRLFNPAESATCDATVAFSREVISCSETMMDEHITTEENQ
                     GSNLSGPFLPGQSRTFSYRLA"
     gene            complement(30547..32463)
                     /gene="mngA"
                     /locus_tag="OO357_000796"
     CDS             complement(30547..32463)
                     /gene="mngA"
                     /locus_tag="OO357_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001393949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS 2-O-a-mannosyl-D-glycerate transporter
                     subunit IIABC"
                     /protein_id="HCR5885840.1"
                     /translation="MNLTTLTHRDALCLNARFTSREEAIHALTQRLAALGKISSTEQF
                     LKEVYRRESLGPTALSEGLAVPHGKTAAVKEAAFAVATLSEPLQWEGVDGPEAVDLVV
                     LLAIPLNEAGTTHMQLLTALTTRLADDEIRARIQSATTPDELLSALDDKGGTQPSASF
                     SNAPTIVCVTACPAGIAHTYMAAEYLEKAGRKLGVNVYVEKQGANGIEGRLTADQLNS
                     ATACIFAAEVAIKESERFNGIPALSVPVAEPIRHAEALIQQSLTLERGDETRTLQQDT
                     QPVKSVKTELKQALLSGISFAVPLIVAGGTVLAVAVLLSQIFGLQDLFNEENSWLWMY
                     RKLGGGLLGILMVPVLAAYTAYSLADKPALAPGFAAGLAANMIGSGFLGAVVGGLIAG
                     YLIRWMKNHLRLSSKFNGFLTFYLYPVLGTLGAGSLMLFVVGEPVAWINNSLTAWLNG
                     LSGSNALLLGAILGFMCSFDLGGPVNKAAYAFCLGAMANGVYGPYAIFASVKMVSAFT
                     VTASTMLAPRLFKEFEIETGKSTWLLGLAGITEGAIPMAIEDPLRVIGSFVLGSMVTG
                     AIVGAMNIGLSTPGAGIFSLFLLHDNGAGGVMAAIGWFGAALVGAAISTAILLIWRRH
                     AVKHGNYLTDGVMP"
     gene            32633..33355
                     /gene="mngR"
                     /locus_tag="OO357_000797"
     CDS             32633..33355
                     /gene="mngR"
                     /locus_tag="OO357_000797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-D-glycerate transport/metabolism system
                     transcriptional repressor MngR"
                     /protein_id="HCR5885841.1"
                     /translation="MGHKPLYRQIADRIREQIARGELKPGDALPTESALQTEFGVSRV
                     TVRQALRQLVEQQILESIQGSGTYVKEERVNYDIFQLTSFDEKLSDRHVDTHSEVLIF
                     EVIPADDFLQQQLQITVQDRVWHVKRVRYRKQKPMALEETWMPLALFPDLTWQVMENS
                     KYHFIEEVKKMVIDRSEQEIIPLMPTEEMSRLLNISQTKPILEKVSRGYLVDGRVFEY
                     SRNAFNTDDYKFTLIAQRKSSR"
     gene            complement(33458..34327)
                     /gene="sucD"
                     /locus_tag="OO357_000798"
     CDS             complement(33458..34327)
                     /gene="sucD"
                     /locus_tag="OO357_000798"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HCR5885842.1"
                     /translation="MSILIDKNTKVICQGFTGSQGTFHSEQAIAYGTKMVGGVTPGKG
                     GTTHLGLPVFNTVREAVAATGASASVIYVPAPFCKDSILEAIDAGIKLIITITEGIPT
                     LDMLTVKVKLDEAGVRMIGPNCPGVITPGECKIGIQPGHIHKPGKVGIVSRSGTLTYE
                     AVKQTTDYGFGQSTCVGIGGDPIPGSNFIDILEMFEKDPQTEAIVMIGEIGGSAEEEA
                     AAYIKEHVTKPVVGYIAGVTAPKGKRMGHAGAIIAGGKGTADEKFAALEAAGVKTVRS
                     LADIGEALKTVLK"
     gene            complement(34327..35493)
                     /gene="sucC"
                     /locus_tag="OO357_000799"
     CDS             complement(34327..35493)
                     /gene="sucC"
                     /locus_tag="OO357_000799"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HCR5885843.1"
                     /translation="MNLHEYQAKQLFARYGLPAPVGYACTTPREAEEAASKIGAGPWV
                     VKCQVHAGGRGKAGGVKVVNSKEDIRAFAENWLGKRLVTYQTDANGQPVNQILVEAAT
                     DIAKELYLGAVVDRSSRRVVFMASTEGGVEIEKVAEETPHLIHKVALDPLTGPMPYQG
                     RELAFKLGLEGKLVQQFTKIFMGLATIFLERDLALIEINPLVITKQGDLICLDGKLGA
                     DGNALFRQPDLREMRDQSQEDPREAQAAQWELNYVALDGNIGCMVNGAGLAMGTMDIV
                     KLHGGEPANFLDVGGGATKERVTEAFKIILSDDKVKAVLVNIFGGIVRCDLIADGIIG
                     AVAEVGVNVPVVVRLEGNNAELGAKKLADSGLNIIAAKGLTDAAQQVVAAVEGK"
     gene            complement(35587..36804)
                     /gene="odhB"
                     /locus_tag="OO357_000800"
     CDS             complement(35587..36804)
                     /gene="odhB"
                     /locus_tag="OO357_000800"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HCR5885844.1"
                     /translation="MSSVDILVPDLPESVADATVATWHKKPGDAVVRDEVLVEIETDK
                     VVLEVPASADGILDAVLEDEGTTVTSRQILGRLREGNSAGKETSAKSEEKASTPAQRQ
                     QASLEEQNNDALSPAIRRLLAEHNLDASAIKGTGVGGRLTREDVEKHLAKAPAKESAP
                     AAAAPAAQPALAARSEKRVPMTRLRKRVAERLLEAKNSTAMLTTFNEVNMKPIMDLRK
                     QYGEAFEKRHGIRLGFMSFYVKAVVEALKRYPEVNASIDGDDVVYHNYFDVSMAVSTP
                     RGLVTPVLRDVDTLGMADIEKKIKELAVKGRDGKLTVEDLTGGNFTITNGGVFGSLMS
                     TPIINPPQSAILGMHAIKDRPMAVNGQVEILPMMYLALSYDHRLIDGRESVGFLVTIK
                     ELLEEPTRLLLDV"
     gene            complement(36819..39620)
                     /gene="sucA"
                     /locus_tag="OO357_000801"
     CDS             complement(36819..39620)
                     /gene="sucA"
                     /locus_tag="OO357_000801"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HCR5885845.1"
                     /translation="MQNSALKAWLDSSYLSGANQSWIEQLYEDFLTDPDSVDANWRST
                     FQQLPGTGVKPDQFHSQTREYFRRLAKDASRYSSTISDPDTNVKQVKVLQLINAYRFR
                     GHQHANLDPLGLWQQDKVADLDPSFHDLTEADFQETFNVGSFASGKETMKLGELISAL
                     KQTYCGPIGAEYMHITSTEEKRWIQQRIESGRATFNSEEKKRFLSELTAAEGLERYLG
                     AKFPGAKRFSLEGGDALIPMLKEMIRHAGNSGTREVVLGMAHRGRLNVLVNVLGKKPQ
                     DLFDEFAGKHKEHLGTGDVKYHMGFSSDFQTDGGLVHLALAFNPSHLEIVSPVVIGSV
                     RARLDRLDEPNSNKVLPITIHGDAAVTGQGVVQETLNMSKARGYEVGGTVRIVINNQV
                     GFTTSNPLDARSTPYCTDIGKMVQAPIFHVNADDPEAVAFVTRLALDFRNTFKRDVFI
                     DLVCYRRHGHNEADEPSATQPLMYQKIKKHPTPRKIYADKLEQEKVATLEDATEMVNL
                     YRDALDAGDCVVAEWRPMNMHSFTWSPYLNHEWDEEYPNKVEMKRLQELAKRISTVPE
                     AVEMQSRVAKIYGDRQAMAAGEKLFDWGGAENLAYATLVDEGIPVRLSGEDSGRGTFF
                     HRHAVIHNQSNGSTYTPLQHIHNGQGAFRVWDSVLSEEAVLAFEYGYATAEPRTLTIW
                     EAQFGDFANGAQVVIDQFISSGEQKWGRMCGLVMLLPHGYEGQGPEHSSARLERYLQL
                     CAEQNMQVCVPSTPAQVYHMLRRQALRGMRRPLVVMSPKSLLRHPLAVSSLEELANGT
                     FLPAIGEIDELDPKGVKRVVMCSGKVYYDLLEQRRKNNQHDVAIVRIEQLYPFPHKAM
                     QEVLQQFAHVKDFVWCQEEPLNQGAWYCSQHHFREVIPFGASLRYAGRPASASPAVGY
                     MSVHQKQQQDLVNDALNVE"
     gene            complement(39921..40637)
                     /gene="sdhB"
                     /locus_tag="OO357_000802"
     CDS             complement(39921..40637)
                     /gene="sdhB"
                     /locus_tag="OO357_000802"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit
                     SdhB"
                     /protein_id="HCR5885846.1"
                     /translation="MRLEFSIYRYNPDVDDAPRMQDYTLEAEEGRDMMLLDALIQLKE
                     KDPSLSFRRSCREGVCGSDGLNMNGKNGLACITPISALNQPGKKIVIRPLPGLPVIRD
                     LVVDMGQFYAQYEKIKPYLLNNGQNPPAREHLQMPEQREKLDGLYECILCACCSTSCP
                     SFWWNPDKFIGPAGLLAAYRFLIDSRDTETDSRLDGLSDAFSVFRCHSIMNCVSVCPK
                     GLNPTRAIGHIKSMLLQRNA"
     gene            complement(40653..42419)
                     /gene="sdhA"
                     /locus_tag="OO357_000803"
     CDS             complement(40653..42419)
                     /gene="sdhA"
                     /locus_tag="OO357_000803"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HCR5885847.1"
                     /translation="MKLPVREFDAVVIGAGGAGMRAALQISQSGQTCALLSKVFPTRS
                     HTVSAQGGITVALGNTHEDNWEWHMYDTVKGSDYIGDQDAIEYMCKTGPEAILELEHM
                     GLPFSRLDDGRIYQRPFGGQSKNFGGEQAARTAAAADRTGHALLHTLYQQNLKNHTTI
                     FSEWYALDLVKNQDGAVVGCTALCIETGEVVYFKARATVLATGGAGRIYQSTTNAHIN
                     TGDGVGMAIRAGVPVQDMEMWQFHPTGIAGAGVLVTEGCRGEGGYLLNKHGERFMERY
                     APNAKDLAGRDVVARSIMIEIREGRGCDGPWGPHAKLKLDHLGKEVLESRLPGILELS
                     RTFAHVDPVKEPIPVIPTCHYMMGGIPTKVTGQALTVNEKGEDVVVPGLFAVGEIACV
                     SVHGANRLGGNSLLDLVVFGRAAGLHLQESIAEQGALHDASESDVEASLDRLNRWNNN
                     RNGEDPVAIRKALQECMQHNFSVFREGDAMAKGLEQLKVIRERLKNARLDDTSSEFNT
                     QRVECLELDNLMETAYATAVSANFRTESRGAHSRFDFPDRDDENWLCHSLYLPESESM
                     TRRSVNMEPKLRPAFPPKIRTY"
     gene            complement(42419..42766)
                     /gene="sdhD"
                     /locus_tag="OO357_000804"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
     CDS             complement(42419..42766)
                     /gene="sdhD"
                     /locus_tag="OO357_000804"
                     /gene_synonym="cybS"
                     /gene_synonym="dhsD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase membrane anchor subunit"
                     /protein_id="HCR5885848.1"
                     /translation="MVSNASALGRNGVHDFILVRATAIVLTLYIIYMVGFFATSGELT
                     YEVWIGFFASAFTKVFTLLALFSILIHAWIGMWQVLTDYVKPLALRLMLQLVIVVALV
                     VYVIYGFVVVWGV"
     gene            complement(42760..43164)
                     /gene="sdhC"
                     /locus_tag="OO357_000805"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
     CDS             complement(42760..43164)
                     /gene="sdhC"
                     /locus_tag="OO357_000805"
                     /gene_synonym="cybA"
                     /gene_synonym="dhsC"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000263334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5885849.1"
                     /translation="MWALFMIRNVKKQRPVNLDLQTIRFPITAIASILHRVSGVITFV
                     AVGILQWLLGTSLSSPEGFEQASAIMGSFFVKFIMWGILTALAYHVVVGIRHMMMDFG
                     YLEETFEAGKRSAKISFVITVVLSLLAGVLVW"
     gene            43545..43652
                     /locus_tag="OO357_000806"
     CDS             43545..43652
                     /locus_tag="OO357_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885850.1"
                     /translation="MRKSYEVGISPKINLCNSVEVLTNSFGTVISGRQV"
     gene            43858..45141
                     /gene="gltA"
                     /locus_tag="OO357_000807"
     CDS             43858..45141
                     /gene="gltA"
                     /locus_tag="OO357_000807"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HCR5885851.1"
                     /translation="MADTKAKLNLNGDTAVELDVLKGTLGQDVIDIRTLGSKGVFTFD
                     PGFTSTASCESKITFIDGDEGILLHRGFPIDQLATDSNYLEVCYILLNGEKPTQEQYD
                     EFKTTVTRHTMIHEQITRLFHAFRRDSHPMAVMCGITGALAAFYHDSLDVNNPRHREI
                     AAFRLLSKMPTMAAMCYKYSIGQPFVYPRNDLSYAGNFLNMMFSTPCEPYEVNPILER
                     AMDRILILHADHEQNASTSTVRTAGSSGANPFACIAAGIASLWGPAHGGANEAALKML
                     EEISSVKHIPEFVRRAKDKNDSFRLMGFGHRVYKNYDPRATVMRETCHEVLKELGTKD
                     DLLEVAMELENIALNDPYFIEKKLYPNVDFYSGIILKAMGIPSSMFTVIFAMARTVGW
                     IAHWSEMHSYGMKIARPRQLYTGYEKRDFKSDIKR"
     gene            45532..46098
                     /locus_tag="OO357_000808"
     CDS             45532..46098
                     /locus_tag="OO357_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5885852.1"
                     /translation="MFKGQKTLAALAVSLLFTAPVYAADEGSGEIHFKGEVIEAPCEI
                     HQDDIDKEVELGQVTTSHINQSHHSDAVAVDLLLVNCDLENSSNGSGGKISKVAVTFD
                     SSAKTTGADPILNNTSTGEATGVGVRLMNKDQSNIVLGTATPDIDLAPTSSEQTLNFF
                     AWMEQIDQATPVTPGAVTANATYVLDYK"
     gene            46149..48608
                     /locus_tag="OO357_000809"
     CDS             46149..48608
                     /locus_tag="OO357_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706516.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5885853.1"
                     /translation="MDTVNIYRLSFVSCLVVAMPCALAVEFNLNVLDKSMRDRIDISL
                     LKEKGVIAPGEYFVSVAVNNNQISNEQKINWHKNDDKTIPCINDLLVDKFGLKPEVRQ
                     SLPLINQCVDFSSRPEMLFNFDQANQQLNISIPQAWLAWHSENWTPPSTWKEGVAGVL
                     MDYNLFASSYRPQDGSSSTNLNAYGTAGINTGAWRLRSDYQLNHTDSDDNHEQSGEIS
                     RTYLFRPLPQLGSKLTLGETDFSPNIFDGFSYTGAALASDDRMLPWELRGYAPQISGI
                     AQTNATVTISQSGRVIYQKKVPPGPFIIDDLNQSVQGTLDVKVTEEDGRVNNFQVSAA
                     STPFLTRQGQVRYKLAAGQPRPSMSHQTENETFFSNEVSWGMLSNTSLYSGLLLSGDD
                     YHSAAMGIGQNMLWLGALSFDVTWASSHFDTQQDERGLSYRFNYSKQVDATNSTISLA
                     AYRFSDRHFHSYANYLDHKYNDSDAQDEKQTISLSVGQPITPLNLNLYANLLHQTWWN
                     ADASTTANITAGFNVDIGDWRDISISTSFNTTHYEDKDRDNQIYLSISLPFGNGGRVG
                     YDMQNSSHSTTHRMSWNDTLDERNSWGMSAGLQSDRPDNGAQVSGNYQHLSSAGEWDI
                     SGTYAANDYSSVSSSWSGSFTATQYGAAFHRRSSTNEPRLMVSTDGVADIPVQGNLDY
                     TNHFGIAVVPLISSYQPSTVAVNMNDLPDGVTVAENVIKETWIEGAIGYKSLASRSGK
                     DVNVIIRNASGQFPPLGADIRQDDSGISVGMVGEEGHAWLSGVAENQKFTVVWGDSQH
                     CSLHLPEHMEDTANRLILPCH"
     gene            48623..49354
                     /locus_tag="OO357_000810"
     CDS             48623..49354
                     /locus_tag="OO357_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706517.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5885854.1"
                     /translation="MTFMKGLPLLLLVASLCSHAALQPDRTRIVFNANDKATSLRVDN
                     RSDKLPYLAYSWLENEKGEKSDDLLVALPPIQRLEPKATTQVRIVKQASTTKLPGDRE
                     TLFFYNMREIPPAPEKNSDHAVLQVAIQSRIKVFWRPAALRKKAGEKVELQLQVSQQG
                     NQLTLKNPTAYYLTIAYLGRNEKGVLPGFKTVMVAPFSTVNTNTGNYSGSQFYLGYMD
                     DYGALRMTTLNCSGQCHLQAVEAKK"
     gene            49351..50427
                     /locus_tag="OO357_000811"
                     /pseudo
     CDS             49351..50427
                     /locus_tag="OO357_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415244.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            50579..51625
                     /locus_tag="OO357_000812"
     CDS             50579..51625
                     /locus_tag="OO357_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415243.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HCR5885855.1"
                     /translation="MPVLQWGMLCVLSLLLSIGFLAVHLPAALLLGPMIAGIIFSMRG
                     ITLQLPRSAFLAAQAILGCMIAQNLTGSILTTLAANWPIVLAILLVTLLSSAIVGWLL
                     VRYSSLPGNTGAWGSSPGGAAAMVAMAQDYGADIRLVAFMQYLRVLFVAGAAVLVTRM
                     MLGDNAEAVNQQIVWFPPVSINLLLTILLAVVAGTAGCMLRLPSGTMLIPMLAGAVLQ
                     SGQLITIELPEWLLAMAYMAIGWRIGLGFDKQILLRALRPLPQILLSIFALLAICAGM
                     AWGLTRFMHIDFMTAYLATSPGGLDTVAVIAAGSNADMALIMAMQTLRLFSILLTGPA
                     IARFISTYAPKRSA"
     gene            complement(51622..52413)
                     /gene="nei"
                     /locus_tag="OO357_000813"
     CDS             complement(51622..52413)
                     /gene="nei"
                     /locus_tag="OO357_000813"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease VIII"
                     /protein_id="HCR5885856.1"
                     /translation="MPEGPEIRRAADNLEAAIKGKPLTDVWFAFPQLKTYQSQLIGQH
                     VTHVETRGKALLTHFPNGLTLYSHNQLYGVWRVVDTGEEPQTTRVLRVKLQTADKTIL
                     LYSASDIEMLRPEQLTTHPFLQRVGPDVLDPNLTPEVVKERLLSPRFRNRQFAGLLLD
                     QAFLAGLGNYLRVEILWQVGLTGNHKAKDLNAAQLDALAHALLEIPRFSYATRGQVDE
                     NKHHGALFRFKVFHRDGELCERCGGIIEKTTLSSRPFYWCPGCQH"
     gene            complement(52449..53183)
                     /gene="pxpA"
                     /locus_tag="OO357_000814"
     CDS             complement(52449..53183)
                     /gene="pxpA"
                     /locus_tag="OO357_000814"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpA"
                     /protein_id="HCR5885857.1"
                     /translation="MKIDLNADLGEGCASDAELLTLVSSANIACGFHAGDAQTMQACV
                     REAIKNGVAIGAHPSFPDRENFGRSAMQLPPETVYAQTLYQIGALATIARAQGGVMRH
                     VKPHGMLYNQAAKEAQLADAIARAVYACDPALVLVGLAGSELIRAGKQYGLTTREEVF
                     ADRGYQADGSLVPRSQPGALIENEEQALAQTLEMVQHGRVKSITGEWATVAAQTVCLH
                     GDGEHALAFARRLRATFAKKGIVVAA"
     gene            complement(53173..54105)
                     /gene="pxpC"
                     /locus_tag="OO357_000815"
                     /pseudo
     CDS             complement(53173..54105)
                     /gene="pxpC"
                     /locus_tag="OO357_000815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308764.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpC"
     gene            complement(54099..54755)
                     /gene="pxpB"
                     /locus_tag="OO357_000816"
     CDS             complement(54099..54755)
                     /gene="pxpB"
                     /locus_tag="OO357_000816"
                     /EC_number="3.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-oxoprolinase subunit PxpB"
                     /protein_id="HCR5885858.1"
                     /translation="MQRARCYLIGETAVVLELEPPVTLASQKRIWRLAQRLVDMPNVV
                     EAIPGMNNITVILRNPESLALDAIERLQRWWEESEALEPESRFIEIPVVYGGAGGPDL
                     AVVAAYCGLSEKQVVELHSSVEYVVWFLGFQPGFPYLGSLPEQLHTPRRAEPRLLVPA
                     GSVGIGGPQTGVYPLATPGGWQLIGHTSLSLFDPARDEPILLRPGDSVRFVPQKEGIC
                     "
     gene            complement(54778..55521)
                     /gene="ybgI"
                     /locus_tag="OO357_000817"
     CDS             complement(54778..55521)
                     /gene="ybgI"
                     /locus_tag="OO357_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000798886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radiation resistance protein YbgI"
                     /protein_id="HCR5885859.1"
                     /translation="MKNTELEQLINEKLNSAAISDYAPNGLQVEGKETVQKIVTGVTA
                     SQALLDEAVRLGADAVIVHHGYFWKGESPVIRGMKRNRLKTLLANDINLYGWHLPLDA
                     HPELGNNAQLAALLGITVMGEIEPLVPWGELTMPVPGMELASWIEARLGRKPLWCGDT
                     GPEVVQRVAWCTGGGQSFIDSAARFGVDAFITGEVSEQTIHSAREQGLHFYAAGHHAT
                     ERGGIRALSEWLNENTDLDVTFIDIPNPA"
     gene            55792..57273
                     /gene="dtpD"
                     /locus_tag="OO357_000818"
     CDS             55792..57273
                     /gene="dtpD"
                     /locus_tag="OO357_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide permease DtpD"
                     /protein_id="HCR5885860.1"
                     /translation="MNKHASQPRAIYYVVALQIWEYFSFYGMRALLILYLTNQLKYND
                     THAYELFSAYCSLVYVTPILGGFLADKVLGNRMAVMLGALLMAIGHVVLGASEIHPSF
                     LYLSLAIIVCGYGLFKSNVSCLLGELYEPTDPRRDGGFSLMYAAGNVGSIIAPIACGY
                     AQEEYSWAMGFGLAAVGMIAGLVIFLCGNRHFTHTRGVNKKVLRATNFLLPNWGWLLV
                     LLVATPALITVLFWKEWSVYALIVATIIGLGVLAKIYRKAENQKQRKELGLIVTLTFF
                     SMLFWAFAQQGGSSISLYIDRFVNRDMFGYTVPTAMFQSINAFAVMLCGVFLAWVVKE
                     SVAGNRTVRIWGKFALGLGLMSAGFCILTLSARWSAMYGHSSLPLMVLGLAVMGFAEL
                     FIDPVAMSQITRIEIPGVTGVLTGIYMLLSGAIANYLAGVIADQTSQASFDASGAINY
                     SINAYIEVFDQITWGALACVGLVLMIWLYQALKFRNRALALES"
     gene            complement(57423..58841)
                     /gene="phrB"
                     /locus_tag="OO357_000819"
     CDS             complement(57423..58841)
                     /gene="phrB"
                     /locus_tag="OO357_000819"
                     /EC_number="4.1.99.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribodipyrimidine photo-lyase"
                     /protein_id="HCR5885861.1"
                     /translation="MTTHLAWFRQDLRLHDNLALAAACRNSSARVLALYIATPRKWET
                     HNMSPRQAELINAQLNGLQIALAEKGIPLLFREVDDFVASVEIVKQVCAENSVTHLFY
                     NYQYEVNERARDVDVERALRNVVCEGFDDSVILPPGAVMTGNHEMYKVFTPFKNAWLK
                     RLREGMPECVAAPKVRSSGSIKPAPSITLNYPRQSFDTAHFPVEEKAAIAQLRQFCEN
                     GAGEYEQQRDFPAVEGTSRLSASLATGGLSPRQCLHRLLAEQPQALDGGAGSVWLSEL
                     IWREFYRHLMTYYPSLCKHRPFIAWTDRVQWQSNPAHLQAWQKGKTGYPIIDAAMRQL
                     NSTGWMHNRLRMITASFLVKDLLIDWREGERYFMSQLIDGDLAANNGGWQWAASTGTD
                     AAPYFRIFNPITQGEKFDREGEFIRRWLPELRDVPGKAVHEPWKWAQKAGVKLDYPQP
                     IVEHKEARVQTLAAYEAARKGK"
     gene            complement(58838..59347)
                     /locus_tag="OO357_000820"
     CDS             complement(58838..59347)
                     /locus_tag="OO357_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbgA family protein"
                     /protein_id="HCR5885862.1"
                     /translation="MNLQRFDDSTLIRIFALHELHRLKEHGLTRGALLDYHSRYKLVF
                     LAHSQPEYRKLGPFVADIHQWQNLDDFYNQYRQRVIVLLSHPANPRDHTNVLMHVQGY
                     FRPHIDSTERQQLAALIDSYRRGEQPLLAPLMRIKHYMALYPDAWLSGQRYFELWPRV
                     INLRHSGVL"
     gene            59476..59763
                     /locus_tag="OO357_000821"
     CDS             59476..59763
                     /locus_tag="OO357_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system SymE family toxin"
                     /protein_id="HCR5885863.1"
                     /translation="MAEHDITPEVTISKTQRRYKVGYVRARHQDRKTGIARYYSQHPS
                     LHLKGDWLKEAGFDTGRGVTVKISEGGLTIIADSDEVQELRDGMFSVLNES"
     gene            complement(59818..60198)
                     /locus_tag="OO357_000822"
     CDS             complement(59818..60198)
                     /locus_tag="OO357_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001515772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885864.1"
                     /translation="MNNLNTLLSGKVLLKEISPLMPGYRTWIEISLIDELKPSYPFRL
                     DEYAMIGHSPYANECNKDEAKFKLRISSFLASDIDNEYDPSYDYVGKYEIIASLNELK
                     TRLSTLHVNLEQFINSSEDDEYPL"
     gene            complement(60201..63587)
                     /locus_tag="OO357_000823"
     CDS             complement(60201..63587)
                     /locus_tag="OO357_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000390557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5885865.1"
                     /translation="MEEAFWAARQGDALLHTSFMADVLGAVLEVAANVVIDALIVGTT
                     SLLATSVGITMGCTAVVLTGFVAGVAMVYTGVTEKVSEACSALANMLFPPQIEGYILT
                     GSGDTWINSKPAARAAATAASRQDIEAQEAQAKAEQEEAQRQEDARTFRDVAGEYLEM
                     AGAIALAVNPVTGPVLMSRALHDQLSTEEGRSEMVDGVTHFVSELWQPTVASAAPGST
                     PTPDDKIDCHKHPSSLTQFLAQKKAAFLDDPLGTALNLLNPGGMLETAFQGVNAVIGS
                     ISNLFKGDDEPPAAEYIAEGARDVRINSQPATRSGARCTCEARVVNEPGNGAFVSPDV
                     RIGGPLLVVRDIRSGRSQITLVATVALMFLRPGKMLSKIACFAASVGMSMLTQKATSA
                     LPHPVNAATGAKYLADDDDFDFSLPGHFPLDWQRVYSSRDERTEGMFGQGWSVMYEVS
                     LVCTPGSADENCMTFVSGMGRRLDMEAVLPGGGFYSPGEGLAVRRGEQGHWLISSDDG
                     QCFLFEADPHHPQRQRLKMLGDRNSNCLNLYYDDRGRIVEISGEQQRPCIRLYYELAA
                     HPRRVTQIYQHFPETAPLLLRRYSYDEAGHLNGVYDSTGHLLREFAYDENHCMTLHRQ
                     PGGEGYYYQWGWYEGPDDAAWRVTGHHTDSGEQYRLAWDLAQRRLCVTDGLGRTRYHQ
                     WDAQNQVTAYQDEAGQVTTFRWSDEERLLLGMTDPQGGKWRYVYDLQGHITETHDPLG
                     RVEQAQWHPVWHQPETEVDAAGNLLGRRAGERATAENSVVPFNRLMSYRGVHYRYDEF
                     GRAVEKEGRSGTQSYRYDAEHRMVEVTTARGTYRYVYDALGRRTEKQHISPDGKPYNR
                     TKFLWDGMRLAQESRPEGTGSLYIYRDQGSYEPLARVDKAGKEGPNRILYFHTDVNGA
                     PEEMTDSDGKIVWETGYQVWGNTIQEKDHGGVEQNLRYQGQYLDRETGLHYNLHRYYD
                     PDVGRFMVTDPIGLRGGLNLYSYAPNPLKYADPLGLTPCAVSNQKANRLLDSSETKVT
                     VRSRSDAEQLFMDRYLGHNYKNMTGESGPSTKNLMEYLTENKTKAGSYHWDDIKDPSV
                     TKPSYRVSGHGPGNPDGDLPHLQVHQHGGSVRHIFFPWET"
     gene            complement(63606..63809)
                     /locus_tag="OO357_000824"
                     /pseudo
     CDS             complement(63606..63809)
                     /locus_tag="OO357_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
BASE COUNT        15691 a        17275 c        15617 g        15601 t
ORIGIN      
        1 gatccagctt ctgcaaccag gccattcaga actgaagtga agcgcagtcg actgttaatt
       61 gacgagtatc tgttaatcag gaaaatagca gacgtccaga acgaatggtt cggcctttgc
      121 atggatcttg cactggtaac aggacagcgc aaaggagatc tggcggctat gagatgggag
      181 gatataagag atagcaggct atacgtggaa caacaaaaaa caggggcaaa aattcgcatt
      241 tctctcccca ccacgatttc cagattgaat ttaacacttg ccgatgtact ggataacttg
      301 aaaaaaataa acggtaaaaa cgaaaagtta ctcggaggga agacagccag gacaattgca
      361 gcacagttca ggattgcccg agacacatcc ggattgaaat gggaaggtga cccaccgcca
      421 tttcatgaaa tccgaagttt atccggtcgc ctgcactcag cagaaaaagg cagtgatttc
      481 actcaggcgc tccttggaca tcgttcatcc agcatgacgg ataaataccg tgacggacga
      541 gggagggaat ggaaagacat ttagaattaa tttcgaatga tttcaaaatg ccacttgtaa
      601 ttaattgatt ataaagaatt aattcctaaa gagcatttgc ttcatgaatg ctcctttgca
      661 tttagcaact gactgtaaca gctaaaatta gtcgcttttg gcggtgaatg ctgaattaaa
      721 acgcagaatt ttgtccgata gtggtatcgg tgtaccattc gcgcagacga aaattcttaa
      781 ccttaatctg gaatacgcca tgaccacacg cgtgattgct ctcgacttag acggcacctt
      841 attgaccccg aaaaagacct tgcttccatc atcgatagaa gccctggccc gcgctcgcga
      901 agcaggctat caattaatca tcgtcacagg tcgccatcac gtcgctattc atccttttta
      961 tcaggcgctg gcgctggata cacctgctat ttgctgtaat ggcacctatt tgtatgatta
     1021 tcatgcaaaa accgtgctgg aagcggaccc aatgcccgtt aataaagccc tgcaactcat
     1081 tgagatgctg aatgaacacc acattcacgg tctgatgtat gtcgatgatg caatggtcta
     1141 tgagcacccg accgggcatg tcattcgcac gtctaactgg gcgcaaaccc tgccgccgga
     1201 acagcgtccg actttcacac aagtcgcttc tctggctgaa acgacgcaac aagttaacgc
     1261 cgtatggaag ttcgccctga cgcacgatga cctgccgcaa ttgcagcatt ttggtaagca
     1321 tgtcgaacat gaactgggac tggagtgtga atggtcctgg cacgatcagg ttgatattgc
     1381 acacggcggc aacagcaaag gtaaacgttt gacgaaatgg gttgaggcgc aaggttggtc
     1441 gatggaaaac gtcgtggcat tcggcgataa ctttaatgat atcagtatgc tggaagccgc
     1501 tggtacaggc gtggcgatgg gcaacgccga tgacgcggta aaagcgcgcg ccaacattgt
     1561 gattggtgat aacaccaccg acagcattgc ccagttcatt tatagccacc tgatttaatc
     1621 aggcggttat cgacacactt ttaatttgcg cgtacagcca caggccaggt ttgatcgcca
     1681 gttcatccct ggcccacggg ctgatacgcg cccacagcgt tttaccgccg acttccagct
     1741 ccacttccac ctggccgttg tcgtcataac tattaacaac ttttgcccgc aatacgttac
     1801 gaatgctggt ttgctgcggc ggttgtaaaa ccagagaaac atcggaagcc tgaatgcgga
     1861 tacgtagcgc agcttgcagc ggttcgccca gcttattgac ccacaaatgc tgatcgccca
     1921 gcgccagcgc ggtcatcgcg taatgcggat gatgctcaag caccgtcact ttcagaatgc
     1981 tactttgttg ctctttcggc agccacggat tcatcacgct actgccccac acttcctcca
     2041 gagcgccaaa ggctttcacc tgaccgtttt ccagtaccat cactctgtct gccagatgga
     2101 ggatctcatc cagcgaatgg ctgacataca acatcggaat gttgatttcc cgtgtcagcc
     2161 gttgcagata aggcaacagt tcgcgtttac gcggaatatc cagtgacgcc agcggttcat
     2221 ccagcaacag caattccggt gctgtcagca aagcccgacc aatcgccacg cgctgttttt
     2281 cgcctccgga caggctgcct ggtaaacggt caagcaacgg ttcaatgcct aaaagcgcca
     2341 ccagcttatc gaactgatcg accatacttt tcgacatgcc gtagcgcaga ttgccacgca
     2401 ctttgtaatg cgggaacaac cgcgcatcct gaaaaacata gccaacacga cgcttttccg
     2461 gcgtcaggca gatacctttt tcggcatcat ttagtacccg cccattgagg acaatccgcc
     2521 ctttttgcgg gcgcgtcagt ccactgatgg cgttaatcag cgaagttttt ccggcaccgg
     2581 agacgccaaa gatagcagtg atgccattgg cgggcagcgt ttcattaatg gtcaggcaat
     2641 ggttgcccaa cgtctgggaa aaattcagct ccagcatgat tagcgccccg cccgttcacg
     2701 gctgattctg gccagccatt ctgaaatcaa cagggagatc atcgccagcg caatagaaat
     2761 aatgcacagt ctcgccgcgc cactttcccc gccgggggtc tggatcaggg tatacatggc
     2821 agaaggaatg gttcgcgttt cgctaggaat gttcgacaca aaggtgatgg ttgcaccaaa
     2881 ctcaccgaga gaacgagcaa aagccagtac cgtaccaaca ataattcccg gtaaggtcag
     2941 cggtaacgtg atagtaaaga aaacgcgcca gcgcccggca cctagtgttc ttgcggcctg
     3001 ttccagtttg acatcaaccc cttccagcgc cagacgaatt gcccgcacca tcagcggaaa
     3061 cgacatgacg gcggcagcga gaaccgcgcc gcgccagcta aaggcgaagg taataccaaa
     3121 ccagtcatac agacgttcac cgataaatcc gcgccgtccc atcgaaacta ataataagta
     3181 accgacgacc acgggcggta acaccagcgg tagatgcagt acgctgtcga gcagagcttt
     3241 gcccggaaac gtgcaacgca ccagtaacca ggcaaaaaag atcccaaacg gcaggctaaa
     3301 cagcacagcc agggaagaaa ctttcaggct taataaaact gcctgccatt ctggatcggt
     3361 cagtatcatt acttggttgt aaatccgtaa cgtttaaaga tttccgctgc ctgcggtccc
     3421 ttcagataat cataaaatgc tttcactgtc gcattgttat gcccttccac aacagcaacc
     3481 ggatattcca cttttttatg tgaatcttcc gggaaggtgg caaccacttt tacccctttg
     3541 ctggcaactg cgtcagaacc gtagacaatg cccagaggcg cttcgttacg ttcgaccagc
     3601 gccagcgccc cacgaacatc ttccgctggg gccagtttcg gagagagcgt atcccatgcg
     3661 cccagttttt gcagtgcttc ttttgcataa atgccagcgg gaacatgttc cggatcgcca
     3721 accgccaggc gaccgccatt cagcagcgaa gtccagttag ttttgctgtc gatggtgaaa
     3781 tctttctgct cgctggcttt cggtgctaca acgaccaggc tattgccgag cagtgtctga
     3841 cgcgtagcag tatcgatcgc ttttttatca accgcataat ccatccattt ctgatcggca
     3901 gaaataaaca gatccgcagg ggcacccgct tcaatctgac gggcgagagt agatgacgaa
     3961 gcgaaagaag aaaccacatc cacgcctttc tcttttttat actgcgtagc aatgtcctgc
     4021 attgcgttag tcagtgatgc ggcggcgaac accgtgattt tcccttcatc tgccagtgca
     4081 ttgccagcaa ctgcgaaaga gagtgccgcc ccggcaaaca agttcaacca tttacgagcc
     4141 attggtaacc ccttaatgta acgttatgta ggcgacaata taacgactaa ctcggaattt
     4201 tcccagcagt tattgctaac ctttgtgagg tagataagaa aaaatatcgg caggaaaagc
     4261 aggaagttga gaaaaagaaa atgcccgact aagcgggcac tcagggaatc aatgattttg
     4321 tccgggctgg tcttttttac caacaccaga aaagatgttg aatacttcac caagaccgta
     4381 aatcagaccc aggatgatgg ccatcacgac aggtaccatg attacggcga ataccagact
     4441 ttttaataac tctaacatgg tcaactccag atatagtcat gagactattc taaccgctaa
     4501 gcacagaaaa gcactcccct tttgtgcggt cagctttgcg tggcgttcgt tttccgtcac
     4561 aataagactt ttgccaggac attgttatgc aggccgaaat ccttctcacc cttaagctcc
     4621 aacaaaaatt attcgccgac ccgcgccgca tttcgctact aaaacacatt gcgctttccg
     4681 gttccattag ccagggagcg aaagatgccg gtatcagcta taaaagcgcc tgggatgcca
     4741 ttaacgagat gaatcagtta agtgagcata ttctggtcga gcgcgcaaca ggcggtaaag
     4801 gtggcggagg cgcagtactg acccgctatg gtcagcgact gattcagctc tatgacttac
     4861 tggcgcaaat ccagcaaaaa gcctttgatg tgttaagtga cgatgacgcc ctgccgctga
     4921 acagcctgct ggccgcgatc tcacgttttt cactgcaaac cagcgcccgt aaccagtggt
     4981 tcggtaccat caccgcccgc gatcatgatg acgttcaaca gcatgttgat gtcttactgg
     5041 ctgacggaaa aacacgcctg aaagtcgcaa ttaccgcaca aagcggcgca cgtctggggc
     5101 tggatgaagg caaagaagtg ttgatattgc taaaagcgcc gtgggtaggc attactcagg
     5161 acgaagcggt cgcacaaaac gccgacaacc aattaccggg tattattagt catattgagc
     5221 gcggcgcaga gcagtgcgaa gtattaatgg cgctacccga cgggcaaaca ctgtgcgcca
     5281 cagtgccggt aaatgaagcg acttctcttc agcaagggca gaatgtcacg gcctacttta
     5341 atgccgacag cgtgattatc gccacgctgt gctaagcgtg ttgacaattt gttatgaaac
     5401 acgtatccct gtcagtaatc gctgcacaaa atgggatata aaatgtcatc gttgcaaatt
     5461 ttgcaaggca cgtttcgtct tagcgacaca aaaacgctgc aattgcctca gctaacgtta
     5521 aacgcgggtg atagttgggc gtttgtcggt tcgaatggaa gtggaaagtc ggcgctggcc
     5581 cgcgcgctgg cgggggaact tccgcttttg aaaggtgaac ggcaaagcca gttttcccac
     5641 atcactcgtc tctccttcga gcaattgcaa aagctcgtca gcgacgaatg gcaacggaat
     5701 aacaccgata tgctcggccc tagcgaagat tacaccggac gcactacggc tgagattatt
     5761 caggatgaag taaaggatgc accgcgttgc atgcaactgg cgcagcagtt cggtattacc
     5821 gccctcctcg accgacgctt taaatacctt tccactggcg agacgcgaaa aaccctgctg
     5881 tgtcaggcgc tgatgtcgga gcctgacttg ttgattcttg atgagccgtt cgatggcctg
     5941 gatgttgcct cacgtcagca gctggctgag cgactcgcct cgttacatca gtccggtatt
     6001 actttggtac tggtgctcaa tcgcttcgat aagatcccgg aatttgtcca gtttgctggc
     6061 gtgctggcgg attgtacgtt agcggaaact ggcgctaaag aggaactgct ccagcaagca
     6121 ctcgtcgcgc aacttgcgca tagtgaacag cttgaaggtg tgcaactgcc ggagccggat
     6181 gaaccttcag cacgtcacgc cttacccgcc aacgaatcgc gcattgtgct gaacaatggc
     6241 gtggtttctt ataatgatcg ccccattctt aataacctta gctggcaggt gaatccaggc
     6301 gaacactggc aaattgtcgg gccaaatggc gcgggaaaat cgacgttatt aagcctgatt
     6361 actggcgatc atccgcaagg ttacagcaac gatttgacgc ttttcggccg acgtcgcggc
     6421 agcagcgaaa ccatctggga catcaaaaag catatcggtt acgtcagcag tagtttgcat
     6481 ctggattacc gggtcagcac taccgtgcgt aatgtgattc tttctggcta ttttgattcg
     6541 attggcattt atcaggtcgt ttcggatcgc cagcaaaaac tggtgcagca gtggctggat
     6601 attctcggca ttgataaaca cacggctgac gctccgttcc atagtctttc ctggggacag
     6661 cagcgtttgg cgctgatcgt ccgcgcactg gtgaaacatc cgacgttgct tattctcgat
     6721 gaaccactac aggggcttga tccgctcaat cgccaactta tccgccgttt tgttgatgtg
     6781 ctgattagcg aaggtgaaac gcaattgttg tttgtttcgc accacgctga agatgcgcct
     6841 gcctgtatta cccatcgcct tgagttcgtg ccggacggtg gactctatcg ctatgtgctg
     6901 acaaaaatat attgagtcgg tagtgctgac cttgccggag gcggcctaag cgcactctcc
     6961 ggccaacggt tcgacgcatg caggcatgaa accgcttctt ttttcagata aaaagcgcaa
     7021 tcattcataa accctctgtt ttataatcac ttaatcgcgc ataaaaaacg gctaaattct
     7081 tgtgtaaacg attccactaa tttattccat gtcacacttt tcgcatcttt gttatgctat
     7141 ggttatttca taccataagc ctaatggagc gaattatgag agttctggtt accggtggta
     7201 gcggttacat tggaagtcat acctgcgtgc aattactgca aaacggtcat gatgtcatca
     7261 ttcttgataa cctctgtaac agtaagcgca gcgtactgcc tgttatcgag cgtttaggcg
     7321 gcaaacatcc aacgtttgtt gaaggcgata ttcgtaacga agcgttgatg accgagatcc
     7381 tgcacgatca cgctatcgac accgtgatcc acttcgccgg gctgaaagcc gtcgccgaat
     7441 cggtacaaaa accgctggaa tattacgaca acaatgtcaa cggcactctg cgcctgatta
     7501 gcgccatgcg cgccgctaac gtcaaaaact ttatttttag ctcctccgcc accgtttatg
     7561 gcgatcagcc ccaaattcca tacgttgaaa gcttcccgac cggcacaccg caaagccctt
     7621 atggcaaaag caaattgatg gtggaacaga tcctcaccga cctgcaaaaa gcccagccgg
     7681 actggagcat tgccctgctg cgctacttca acccggttgg cgcgcatccg tcgggcgata
     7741 tgggcgaaga tccgcaaggc attccgaata acctgatgcc atacatcgcc caagttgctg
     7801 taggccgtcg cgactcgctg gcgatttttg gtaacgatta tccgaccgaa gatggtactg
     7861 gcgtacgcga ttacatccac gtaatggatc tggcggacgg tcacgtcgtg gcgatggaaa
     7921 aactggcgaa caagccaggc gtacacatct acaacctcgg tgctggcgta ggcagcagcg
     7981 tgctggacgt ggttaatgcc ttcagcaaag cctgcggcaa accggttaac tatcattttg
     8041 caccgcgtcg cgaaggcgac cttccggcct actgggcgga cgccagcaaa gccgaccgtg
     8101 aactgaactg gcgcgtaacg cgcacactcg atgaaatggc gcaggacacc tggcactggc
     8161 agtcacgcca tccacaggga tatcccgatt aaggaacgac catgacgcaa tttaatcccg
     8221 ttgatcatcc acatcgccgc tacaacccgc tcaccgggca atggattctg gtttcaccgc
     8281 accgcgctaa gcgcccctgg cagggggcgc aggaaacgcc agccaaacag gtgttacctg
     8341 cgcacgatcc agattgcttc ctctgcgcag gtaatgtgcg ggtgacaggc gataaaaacc
     8401 ccgattacac cgggacttac gttttcacta atgactttgc ggccttgatg tctgacacgc
     8461 cagatgcgcc agaaagcaac gatcctttga tgcgttgcca gagcgcgcgc ggtaccagcc
     8521 gggtgatctg cttttcaccg gatcacagta aaacgttgcc agaactgagc gttgcggcat
     8581 tgacggaaat cgtcaaaacc tggcaggagc aaaccgcaga gctgggaaaa acgtacccgt
     8641 gggtgcaggt ctttgaaaac aaaggtgcgg cgatgggctg ctctaacccg catccgcacg
     8701 gacagatttg ggcaaatagc ttcctgccta acgaagctga gtgcgaagac cgcctgcaaa
     8761 aagaatattt tgccgaacag aaatcaccaa tgctggtgga ttatgttcag cgcgagctgg
     8821 cagacggtag ccgtaccgtt gtcgaaaccg aacactggtt agccgttgtg ccttactggg
     8881 ctgcctggcc gttcgaaacg ctactgctgc ccaaagccca cgttttgcgg atcaccgatt
     8941 tgaccgacgc ccagcgcagc gatttggcgc tggcgttgaa aaagctgacc agtcgttatg
     9001 acaacctctt ccagtgctcc ttcccctact ctatgggctg gcacggcgca ccatttaatg
     9061 gcgaagagaa tcaacactgg cagctgcacg cgcactttta tccgcctctg ctgcgctccg
     9121 ccaccgtacg taaatttatg gttggttatg aaatgctggc agagacccag cgagacctga
     9181 ccgcagaaca ggcagcagag cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg
     9241 gagtgtaaga aatgagtctg aaagaaaaaa cacaatctct gtttgccaac gcatttggct
     9301 accctgccac tcacaccatt caggcgcctg gccgcgtgaa tttgattggt gaacacaccg
     9361 actacaacga cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg atcagctgtg
     9421 caccacgcga tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat cagctcgacg
     9481 agttttccct caatgcgccc attgtcgcgc atgaaaacta tcaatgggcg aactacgttc
     9541 gtggcgtggt gaaacatctg caactgcgta acaacagctt cggcggtgtg gacatggtga
     9601 tcagcggcaa tgtgccgcag ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg
     9661 tcggaaccgt attgcagcag ctttatcatc tgccgctgga cggcgcacaa atcgcgctta
     9721 acggtcagga agcagaaaac cagtttgttg gctgtaactg cgggatcatg gatcagctaa
     9781 tttccgcact cggcaagaaa gatcatgcct tgctgattga ctgccgctca ctggggacta
     9841 aagcagtttc catgccgaaa ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta
     9901 ccctggttgg cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct
     9961 tccagcagcc agctctgcgc gatgtcacca ttgaagagtt caacgctgtt gcacatgagc
    10021 tggacccaat cgtggcgaaa cgcgtgcgtc atatcctgac tgaaaacgcc cgcaccgttg
    10081 aagctgccag cgcgctggag caaggcgacc tgaaacgtat gggcgagttg atggccgagt
    10141 ctcatgcctc tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac actctggtag
    10201 aaatcgtcaa agctgtgatt ggcgacaaag gtggcgtacg catgaccggc ggcggatttg
    10261 gcggctgtat cgtcgcgctg atcccggaag agctggtgcc tgccgtacag caagctgtcg
    10321 ctgaacaata tgaagcaaaa acaggtatta aagagacttt ttacgtttgt aaaccatcac
    10381 aaggagcagg acagtgctga acgaaactcc cgcactggca cccgatggtc agccgtaccg
    10441 actgttaact ttgcgtaaca acgcagggat ggtagtcacg ctgatggact ggggtgcgac
    10501 tttactttcc gcccgtattc cgctttccga tggcagcgtc cgcgaggcgc tgctcggctg
    10561 tgccagtccg gaatgctatc aggatcaggc tgcgtttctg ggggcctcta ttggtcgtta
    10621 tgccaaccgc atcgccaata gtcgctatac ctttgacggt gaaaccgtga cgctttcgcc
    10681 aagccagggc gttaaccagc tgcacggcgg cccggaaggg ttcgacaaac gtcgctggca
    10741 gattgtgaac cagaacgatc gtcaggtgct gtttgccctg agttcagatg atggcgatca
    10801 gggcttcccg ggtaatctcg gcgcgacggt gcaatatcgt ctgaccgacg ataaccgtat
    10861 ctccattact tatcgcgcca cagttgataa accttgcccg gtgaatatga ctaatcacgt
    10921 ctatttcaat cttgacggcg agcagtctga cgtgcgcaat cacaagttgc agattctggc
    10981 ggacgaatat cttccggttg atgaaggcgg cattccgcac gacggcttga aatctgtcgc
    11041 cggaacgtct tttgatttcc gcagcgccaa aatcatcgcc agtgagtttc ttgccgacga
    11101 cgatcagcgc aaagtgaaag gttacgatca cgcattcttg ttacaggcca aaggcgatgg
    11161 caagaaagtg gcggcgcatg tctggtcagc agatgaaaaa ttgcagctga aggtctacac
    11221 caccgctccg gctcttcaat tctactccgg caacttcctc ggcggcacac cgtcgcgggg
    11281 aaccgaacct tacgccgact ggcaagggct ggcgttggaa agcgagtttc tgccggacag
    11341 cccgaaccac cctgaatggc cgcaaccgga ctgcttcctg cgtcctggcg aagagtattc
    11401 cagcctgacg gaatatcagt ttattgctga gtaatgttaa gccctcgtca tgagggcttt
    11461 atcttcatat tgttcaaatc accagcaaac accgacatat ttgcaactca atattcacaa
    11521 caaccttaca ctgcgccact attttcgcta tggttatgcg taagcattgc tgttgcttcg
    11581 tcacggcaat ataatgagaa ttattatcat taaaagatga tttgaggagt aagcatatgg
    11641 ctgtaactaa gctggttctg gttcgtcatg gcgaaagtca gtggaacaaa gaaaaccgtt
    11701 tcaccggttg gtacgacgtg gatctgtctg agaaaggcgt aagcgaagca aaagcagcag
    11761 gtaagctgct gaaagaggaa ggttacagct ttgactttgc ttacacttct gtgctgaaac
    11821 gcgctatcca taccctgtgg aatgtgctgg acgaactgga tcaggcatgg ctgcccgttg
    11881 agaaatcctg gaaactgaac gaacgtcact acggtgcgtt gcagggtctg aacaaagcgg
    11941 aaactgctga aaagtatggc gacgagcagg tgaaacagtg gcgtcgtggt tttgcagtga
    12001 ctccgccgga actgactaaa gatgatgagc gttatccggg tcacgatccg cgttacgcga
    12061 aactgagcga gaaagaattg ccgctgacgg aaagcctggc gctgaccatt gaccgcgtga
    12121 tcccttactg gaatgaaacc attctgccgc gtatgaagag cggtgagcgc gtaatcatcg
    12181 ccgcacacgg taactccctg cgtgcgctgg tgaaatatct tgataacatg agcgaagaag
    12241 agattcttga gctgaatatc ccgactggcg tgccgctggt gtatgagttc gacgagaatt
    12301 tcaaaccgct gaaacgctat tatctgggta atgctgacga gatcgcggcg aaagcagcag
    12361 ccgttgcgaa ccagggtaaa gcgaagtaaa cgtcattcgt ttaaaaaatg agaaagccga
    12421 ctgcaagtga gtcggctttt ttgtttgtcg ttgctaacaa tggagcacat tgcctgatgc
    12481 gacgctgcgc gtcttatcag gcctgtggtg attcatcgga tacgccactc tgacggcgca
    12541 tccgacaatt aaaccttacc cgcgacgcgc ttttactgca ttcaccagtt gacgtaacag
    12601 agcatcggta tcttcccagc caatgcaggc atcggtgatg ctcttaccgt aggccagcgg
    12661 ctccccgcta tcgaggctct gattgccttc caccagatgg ctttccacca tcacgccaat
    12721 aatggccttt tcgccaccgg caatctgctg gcaaacgtca gcacaaacat ccatctgctt
    12781 tttgaattgt ttggacgaat tagcatggct gaaatcgatc atcacctgag ctggcagacc
    12841 tgctttgttc agcccttctt tcacttcagc aacgtgcttc gcgctgtagt taggctcttt
    12901 accgccgcgc agaatgatat ggcaatcgcc gttaccactg gtattcacaa tcgccgaatg
    12961 cccccattta gttacggaca ggaagcagtg cggcgcaccg gcggcattaa tggcatcgat
    13021 agccacttta atcgtaccgt ctgtgccatt tttaaaacct accggacaag aaagaccaga
    13081 cgccagttcg cggtgcacct gcgattccgt ggtacgtgca ccaattgcgc cccagctcat
    13141 caggtcagcg agatactgag gagtgatcat atcgagaaac tcacccgccg ctggcagacc
    13201 gctgtcgtta atatcaagca gcaatttacg ggctatacgc agaccgtcgt tgatctggaa
    13261 gctgttatcc atatgcggat cgttaatcaa ccctttccag cccaccgtgg tacgcggctt
    13321 ttcaaaatag acgcgcatta cgatttccag ctcatctttc agctcttcac gcagcgccag
    13381 caagcgagtg gcatactctt tagccgcgac aggatcatga attgagcacg ggccaatcac
    13441 caccaacagg cgatcatcat tacctttcag gatcttatgg atcgcttttc gggcatgggc
    13501 aaccgtattc gcggcatttt cagttgcggg gaatttttcc agcaatgcga caggaggaag
    13561 taactctttg atttctttga tgcgtaaatc gtcgttctga taattcatgt ctgttccagt
    13621 gttgccatac ttatcttagt gaatgcaata cttccaatct atatcttccg tcagaatgtg
    13681 taaacggggt tttacactat gaacggatta atcctggaat tagcaagaaa aacgccagat
    13741 tgtcgcgaaa aacgagatct ctcctacaat ttctaactgt aactcctttg tttgttaatt
    13801 atttcaagat tctctgctgc gtttcataaa ctggctgaaa tcttaaacca atgccttata
    13861 ttcacctgca aatgcactgt tggaagaggt tatccgacat aacgaccata acaggagcat
    13921 cctatgaaaa tgacaaaact ggccacatta tttctgactg ccactctaag ccttgccagc
    13981 ggtgccgcac tggctgccga tagcggagcg caaactaata acggccaggc aaacgccgca
    14041 gctgatgcgg gccaggtagc ccctgacgcc cgtgaaaatg tcgcgccaaa taacgtcgac
    14101 aataacgggg taaataccgg ttctggcggc acaatgctgc attcggatgg ttcttcaatg
    14161 aacaatgacg gaatgaccaa agatgaagag cacaagaaca ccatgtgcaa agatggtcga
    14221 tgtccggata tcaataaaaa ggtacaaacc ggtgatggca tcaataatga tgtcgatacc
    14281 aaaaccgacg gtaccacgca gtaaccctgc aaaatagcca ataatgggag agcggagtct
    14341 ctccctttta atttttcact tctaaaagca gtatgatgtc ataaagttaa gtgagaataa
    14401 ttaagggatg actcgtcatg gcgcactcac actcacacac gtcctcacac ctgccagaag
    14461 ataataatgc tcgtcgcttg ttgtatgctt tcggcgtgac tgccgggttt atgctggtag
    14521 aagtcgttgg tggttttctt tctggttctc tggcattgct ggccgatgcg ggtcatatgt
    14581 tgaccgatac tgccgccctg cttttcgccc tactcgccgt gcaattttca cgtcgtcctc
    14641 ccaccattcg ccacactttc ggttggctaa gactgaccac gctcgcggcg tttgtgaatg
    14701 ccatcgccct cgtggtaatt accattttga ttgtctggga ggcgatagaa cgcttccgca
    14761 cgccgcgtcc ggtcgaggga ggcatgatga tggcaattgc cgtggctggg ttgctggcaa
    14821 atatactttc tttctggtta cttcatcacg gcagcgaaga gaaaaacctc aacgtacgag
    14881 cagcggcact gcatgtgctc ggggacctgc tgggttcggt tggggctatt attgccgcgt
    14941 tgattattat ctggactggc tggacacctg ctgaccccat tctctcgata ctggtgtcgc
    15001 ttctggttct gcgtagcgcg tggcgactgt tgaaagatag cgtgaatgaa ttacttgaag
    15061 gtgcaccggt atcgctggat atcgctgaac tgaagcgtcg tatgtgccgg gaaatcccgg
    15121 aagtccgcaa tgtgcaccat gtacatgtat ggatggtagg cgagaagccg gtgatgacgt
    15181 tgcatgtgca ggtgatcccg ccacacgatc acgatgcctt gttggatcag atccaacact
    15241 acctgatgga tcactatcag attgagcaca ccaccattca gatggaatat caaccttgtc
    15301 ataggccaga ctgccacctg aatgaagggg tgtctggcca ttcacatcat caccattaat
    15361 gggacagcgc gcgtgagcct ctttcacgtg cgctgttgat ccacatccgg ctgccgttga
    15421 gcgcaataaa ggtcaggatg atgtactcca gagacatggc gtaaacgccc tgaagtgcaa
    15481 aaataacgac gctaatcacg ttaataatca cccacaacag ccagttttcc acatacttac
    15541 gcgtcatcag aatcattgcc acgatagata acaccatcat gcatgaatcc cagaacggga
    15601 aagcgtccgg ttgcagttca ggcatcacaa cctgtaatcc taatgcttgc atgatcatga
    15661 ctgccacgcg ggtcaaaaat gcaaacaccg gattgataaa taccgtcatc agaccaatcg
    15721 aaacaacgca aaccgccaac cagctgagtg ccttcggcaa tggcaaccag cgaattttca
    15781 actccgcctc gttctgactg gtttgtcgcg accacgcata ccagccgtaa atattcgcgg
    15841 caaagaaaaa cacctgtaat agcaggctgg catacagctg aatctgaaag aaaataatgc
    15901 caaacaaggt aacgttgatc aggccaaaga agtagttgct gatcttctcc agactggcaa
    15961 ggccaataca cagcaacccg gcgatcgtgc ctaccgcttc gatccatgag agatcataac
    16021 cgcccgcccc tattggtata tgtaccagga tattctgcac actaaaaaaa tccatctttc
    16081 ccccaaagcg taacgttacg ccttattatt cgttatccac gtagtgtagc cgcaaaatcc
    16141 agcatacgat tgagcggcac cagcgccctc tctcgcagcc tctcatcgac atgaacctcg
    16201 tgattacttc cttcctgttc taatgcctct gcgatagcct gaaggtcatt catggccatc
    16261 cacggacaat gcgcgcagct gcggcaggtt gcgccctcac ccgcggttgg tgcttccagt
    16321 aactctttat ctggcaccgc ctgctgcatt ttgtagaaaa taccccgatc ggttgccaca
    16381 ataagcctct gatgaggcaa tgcttttgca gcagcgatca gttgactggt ggaaccgacc
    16441 gcatccgcca tatcgacaat agcttgtggt gattctggat gcaccagtat ggcagcatcc
    16501 gggtattctt cttgcaagcg ggttaacgcc tgagtcttaa attcatcatg cacaatgcag
    16561 gcaccctgcc agcataaaat gtctccaccc gtctgttttt gcacgtaacg ccccagatgt
    16621 ttgtcgggtg cccagatgat tttttcaccc aaactatcaa gatgatcaat aagttcgacg
    16681 gcaatgcttg aagttaccac ccaatctgcg cgcgctttta ccgcagcaga agtgttggcg
    16741 tagacgacga cagtacgatc gggatgggca tcgtaaaatg cgttaaattc ttcaacaggg
    16801 cagccgagat ccagtgaaca ctcaacctga agtgtcggca tcagaattgt tttttccgga
    16861 ctgagaattt tggcggtttc tcccataaat ctcaccccag cgactaacaa agtagaagcg
    16921 ggatgctttg caccgaagcg cgccatttcc agagaatcag aaatacagcc accggtttct
    16981 tctgccagtt gttgaatttc gggatcggta tagtagtggg caaccatcac cgcattacgt
    17041 tcttttagca gacgttttat cttctcgcgg taatacgctt tttcatcaat gcttaacggc
    17101 gtcggcttcg gggggaaagg ataaatcgcc gtgtctggat caaacattac gctcatctta
    17161 ccatctcgtt ttacaggctt aacgttaaaa ccgacattag cgtaacattc gctgttatcg
    17221 gtgtaatttg ttttatatgc taaacaagat agtcgaatgg tggggaaaag tcactcgaat
    17281 tacgcctaat tccaccgacg tagagttgct tgcacagtaa gctctgacga tgtcatcagg
    17341 tttacaaagt gaaattgagg atgcctgaat agcaaaaaag cgcctttagg gcgctttttt
    17401 gcattggtgg gtcgtgcagg attcgaacct gcgaccaatt gattaaaagt caactgctct
    17461 accaactgag ctaacgaccc gagtggtggg tgatgacggg atcgaaccgc cgaccccctc
    17521 cttgtaaggg aggtgctctc ccagctgagc taatcacccg atactgcgct ggatactaca
    17581 aactacttcc accgaaccca ccgtaattag tggtgggtcg tgcaggattc gaacctgcga
    17641 ccaattgatt aaaagtcaac tgctctacca actgagctaa cgacccggtg gtgggtgatg
    17701 acgggatcga accgccgacc ccctccttgt aagggaggtg ctctcccagc tgagctaatc
    17761 acccgacact acgctggata ctacaactaa ctatgttagt gggggtcgtg caggatgact
    17821 cgacttcgtc tcgcccttcg gcgccgttgc tgatgcaacg ttatccttca cgttttacta
    17881 ccgcttccac cttagcgatt ggtgggtcgt gcaggattcg aacctgcgac caattgatta
    17941 aaagtcaact gctctaccaa ctgagctaac gacccacttt ttcgttgctt tcggtttgtt
    18001 tgatatcccg tggcaacggc ggcatatatt actgatttca gacttgagcg caacaaaaat
    18061 ttcgatgcag atcactcaac tgcttatgat tcgcacgaca cgaccagaaa taatgcgact
    18121 tctggtcgtg tgttatgcat tacatcgcgt tcagacgttt ttgtgcctgt ttagcgccat
    18181 cagtaccagg gtatttactg ataacctgct ggtatacggc tttcgctttt gcggtgtcac
    18241 ctttgtcctg catgatgacg ccgactttaa acatcgcatc agcagccttt ggtgacttcg
    18301 gatagttttt cactaccgaa gcaaaatagt acgccgcatc atctttttta cccttgttgt
    18361 agtttaactg acccagccaa taattggcgt ttggcaggta agttgaatca gggtaatttt
    18421 tgatgaaatt ctgaaatgcc accattgcgt catcctggcg ggatttatcc tgcaccagcg
    18481 caatagctgc attgtaatcc gtgtttgcat cgccgctttt caccggcgcg ccagcattcg
    18541 cagtaccagc atcagctgtc ggcgtcgttg atgccgtcgc accgctttga tcgctgctgg
    18601 ttgattgcgc cgctgcacca ccgctgctga ggctgtcgat ctgcaacagg atctgctttt
    18661 gccgctccac gacctgattc agttgatact gattttcctg aatctgacca cgcagggaat
    18721 caatatcgga ttgattatca gaaagttgtt gctggagttg ggttaaaagc tggctgtgag
    18781 cattagaaat acgctcaagt tgagtgacgc ggtcttcgac cgagcctgag ctgacactac
    18841 tgattggtgc ctgagcaaaa gcggcccagg gggccgctat accaaccagt aacgacagac
    18901 tcaatagttg atgtctgaag ttactgctca tgcaattctc ttagtaaacc agtaccgcac
    18961 gacggttttt ggagtatgcc gcttcgtcat gacccagtac tgcaggtttt tctttaccgt
    19021 aagaaacgat ggagatctgg tctgcagaaa cgcctttacc ctgcaggtac atcttaacgg
    19081 cgttcgcacg acgttcaccc agggagatgt tgtattccgg agtaccacgt tcgtccgcgt
    19141 gaccttctac ggtgactttg taagacgggt tgctacgcag gaagtttgca tgtgcatcca
    19201 gcatttgagc gaagtcagaa cggatatcgt acttgtccag atcgaagtaa acgatgttgt
    19261 tctgctgcag ctgttgcatt tgcagacgag cctgctcttc ggaagacatg ttgccgttgc
    19321 cgccgttcgc atccatacca gtgccggcac ccagcatgcc ttcgctgccg tcattgctgg
    19381 cgttcttgtt ggaagaacat gccgcaattg ccataacagg cagagcaatc atcagccctt
    19441 tcagcacttt gttcagttgc atttcaatga ttcctttact attcaattaa ttattatcac
    19501 agatacggcg accaggcagg gaatttgacc tgtccatcag ttgccggaag acgcgctttg
    19561 aaacgcccat ctgtagaaac caaattcagc acggatccca tcccctgaga agagctgtag
    19621 attaccatag tgccgttagg tgccagactt ggcgtttcat ccaggaacgt ggacgacaga
    19681 acttgtacgc ctcccgttgc cagatcttgt ttggcaatgt gctgctgccc accattggag
    19741 ctgaccatta ccataaattt accgtcgctg ctgacatccg catcctggtt ctgcgaacct
    19801 tcccaggtaa tacgttgtgg cgcaccgccg ttgatattca ctttataaac ctgcggacga
    19861 ccggcctggt cagaagtaaa tgccaggttc tggctatccg ggaaccaggt cggttcggta
    19921 ttgttactgc gaccatcagt cacctggcgg atctgaccag aagccaaatc cattacgtac
    19981 aggttcagac taccggtttt cgacaaggcg aatgccagtt tgctgccgtc tggcgagaat
    20041 gcaggtgcac cgttgtgacg cgggaatgaa gccacctgac gtacagcgcc atttgccagc
    20101 gtctgaataa ccagcgcgga acgaccgctt tcgaaggtca cataagccag tttagaaccg
    20161 tctggtgacc acgccggaga catcagcggc tgcggtgaac ggtgaacgac aaactggttg
    20221 taaccgtcat agtcagatac gcgcagttca tacgggaact gaccgccgtt ggtctgaaca
    20281 acgtaggcaa tacgggtacg gaacgcacct ttaatgccgg tcagtttttc aaacacttca
    20341 tcactggcgg tatgaccagc ataacgcagc cactgcttgt tcactttgta cgagttctga
    20401 gcaagtacag tacccggtgc gccgccagtg tcaacaagtt gataagcaac attgtaagaa
    20461 ccatccggat tcggagtgac ttgaccgaca actacagcgt caatgcccag tgcggaccat
    20521 gcagctggtt gtacttcctg cgcactaccc ggctgctgtg gcagacgagc gcgatctaac
    20581 ggattaaatt taccgctgtt acgcaagtca gcagcaacga tgccgccaat atcttcaggt
    20641 gccgcaccag gccccgccca ctggaaagga acaacaccaa taggacgacc ggaatctaca
    20701 ccgctgtcga tcacaatgcg gacttcagca tgcagaactg atgcccacag tatgagaaaa
    20761 ccaaatgcta ctcgtaatgc ctgcttcatc atatctccct tatctggacc gatggcccac
    20821 gataatttag cagaatgtta acaaactaaa atacacaaaa ctaccagaac cccgtggcaa
    20881 ccggtgcctg atgttgaccg tccgaacagt caacatcgcg attacggttt gaagtccaat
    20941 ggcgcgtttt tgaacacttc atatactgcc tggcttggtg gtttcgggat cttcgcaagt
    21001 ttagctgctg ccaacgcagc ctgacaaagt gcgggatcgc caccttcagg tttgatatcc
    21061 agtaacatac catcgggtgc cagttttatg cgcagcgtac aggttttgcc tgcataggac
    21121 gatgcgtcat agaacttact ttcgatagca gatttaatct gcccggcata gttattgata
    21181 tcggcccctg atgcgccatt gtttttagta ttaccactcc cggcaggcga agcattgttc
    21241 cctttcgccc ctccccccgt tttcggtgca ttcttaccag agcttagctc accgaaaata
    21301 tcatctgcct ctgcggcagc ttttgctgca gcggcttttt cagctgctgc ttttgccgct
    21361 gccgcttttt tgtctgcggc ggctttttct gccgctgctt tcttatcagc tgctgcctta
    21421 tcagcagccg ctttttctgc tgcttttttg tcggctgcag ctttctctgc tgccgctttc
    21481 ttatcagctg cagccttttc agcagccgct ttcttctcag cttctgcctt ggctttttca
    21541 gcagcttcag ttgccgcttt ctttcttgct tcagctgcag ctgcttctgc cgcttccgct
    21601 ttcttcttca gtgccgcagc ggctacctcg gcttttttct gcgcttcggc tgcggctttg
    21661 gcggcttctg cttctgcttt tttcttcgcg tctgcagccg ctttcttcgc tgcttcttcc
    21721 gcagctttag catctgccgc cgctttcgct gccgccactt cagcttgctt ctgctttaac
    21781 tcggcctgtt ttgcggcttc ttcagcctgc tttttctgct cctgagccgc taaccgctct
    21841 ttctcaagtt gcttcaggcg ttcctgttca gccgcttgtt tctcacgcag ttcttcagca
    21901 gcctgctgtt ccttcatctt gcgctgttca tcagaacgct tcgcgcttga ttcctggctt
    21961 tgcatgcgtt tgtactgctc aactaccgca cctgaatcaa ccatgacagc gtcgatggac
    22021 gaaccaccgc cgcctccggc tgaagcttct atattctcat cgaacgaact ccagatcagc
    22081 gccgcaaata agatgacatg cagcactgct gaaataatta tcgcccgctt gagcttgtcg
    22141 ttttgttcgg ttgcctttga cactctcggt ttccaaaaac tgttcgcctg ttacccgctc
    22201 tctttcaagc aagggaaacg cagatgttta gataggctgc gtcattaaac caaccgattt
    22261 cacacccgca ctatgtaaca agttcagtgc tttaattatt tcatcgtaag gcacatcttt
    22321 tgcgccaccg atcagaaaga ccgttttcgg gttggccttg aaacggctgg acacttccgc
    22381 caccacctgc tctggtggta aacgctccag gcgatctttc tcaaccacca cggtgtactg
    22441 accaatacca gacacttcaa caatcactgg cggattatcg ttactgctca ccgcctgtga
    22501 ttcagtagcg tctggcagat cgacctccac gctctgggtg atgatgggcg ctgtcgccat
    22561 aaagatcagc aacagcacca gcagtacgtc cagcaacgga acaatgttga tttcggactt
    22621 gagatcgcga cgacctcgtc cacgcgctct ggccatggct taccccttgt tgctctcgct
    22681 aacggtaaac gcctggcggt gcagaatcgc ggtaaactct tccataaagt tgtcgtaatt
    22741 cagttccagt ttgtttacgc gctggttgag acggttataa gccattaccg ccggaattgc
    22801 ggcgaacaga ccaattgccg tcgcaatcaa cgcttctgcg ataccgggcg caaccatttg
    22861 cagtgttgct tgttttaccg ccccgagggc gataaaggcg tgcatgatcc cccagaccgt
    22921 accaaacaga ccaatatacg ggctgatgga gccaaccgta ccgaggaacg gaatgtgcgt
    22981 ttccagattt tcaagttcac ggttcatgga gatacgcata gcacgcgacg ccccttccac
    23041 tacggcttcc ggcgcatggc tattggcacg atgcaggcgc acaaactctt tgaacccgct
    23101 gtaaaagatt tgttccgaac cagtcagatt atcccgtttc ccctgactct cttgatagag
    23161 gcgagagagt tcgattccag accagaattt atcttcaaac gcttcggctt cacgcgccgc
    23221 tgcgttaaga atacgggttc gctggataat aatggcccag gatgcgattg aaaaaccaat
    23281 caaaatcaac atgataagtt taaccagaag gctagccttc aggaacaaat caaggatatt
    23341 catgtcagtc actgcttaaa ctccgcgaca atagacttgg gaagcgcacg aggcttcatt
    23401 ttgagtgggt caacgcaaac aaccagaacc tctgcttcat tcagcaaagt attctcggcg
    23461 ttgacaatac gttgcgtgaa aaccaaagag gtgccacgca ttgatgttat ttcagtctgt
    23521 atttcgagca tatcgtcgag ccgcgcaggt gcgtaatatt ccaccgtcat tttacgtacc
    23581 acaaaggcaa cgcgttcagc catcagcgcc tgctgactga agtgatgatg acgcagcatc
    23641 tctgtgcgtg ctctttcata aaaagcgacg taactggcgt ggtacaccac accaccggca
    23701 tcggtatctt catagtagac gcgaaccggc catcgaaaca gcgttgtatt cactttacat
    23761 cccggtaatg caacaaaagt tagagctttt aaacgctgct actatacgcg cgcgattgag
    23821 gtttgggaat gagggcaagt taagggcgcg taaaaattta tgggcctaga gcggcccata
    23881 attttttaaa gagaattctg atttaaaaga agaagaaaat cagcccgaca atgagaacaa
    23941 tatcggcaag caatgggcaa aaaatccctt gccaaagaac cttctgcgga cgaaagccca
    24001 cgccgtgaat cacaccagca cagacggccc acatcagcaa taaaccatgc cagatttcca
    24061 gttcactggt cttcgcggca aaacgagatg ggtcccaaaa catacatcct gctaacagaa
    24121 gcgccatcac gaaggaaagc gcccgcaggg ggcgcttgtc cattaccgca tacaaggtcg
    24181 cgataatctt actcatcaga tgtcttcttg accggctttg cctgattcga cgtgttcaag
    24241 cgccagcgcg gtgattaccc caaacgaaca ggcaagaagc gttcccagaa tccatgcgaa
    24301 ataccacatt tttagctcct tacttagtac agagagtggg tgttacgttc aatatcttct
    24361 ttggtgatac gaccgaacat tttccagtaa caccaggcgg tgtagagcag aatgatcggt
    24421 accagaacca ccgcaaccca ggtcatgacg ttaagcgtca gctggctgga agttgcatcc
    24481 cacattgtca gacttgcgtt catcatggtg ctggacggca tcacaaacgg gaacattgcg
    24541 ataccggctg tcaggatgat gcaggccagc gtcagggagg agaacacaaa cgcccacgcg
    24601 gctttatcca tacgtgcagt caggatggtc agcagcagca gaaccacacc cagtgccgga
    24661 atagcccaca gaattggcgt gttgttgaag ttaaccagcc atgcgccagc ttcacgaacc
    24721 acttctttat tcagtgggtt agaggctgcg taatggtcca ttgtcgattt cacgacataa
    24781 ccatcgatac cgtacatcac ccatacgcca gccagtgcga aacagaccag tgtcaccagc
    24841 gcagccacct gagccgttgc acgggtacgc aggtgcagtt cgcccacggt acgcatttgc
    24901 agataggttg cgccctgagt aatgatcatc cctacgctca ccacgcctgc cagcaggccg
    24961 aacgggttaa gcaactggaa gaagttaccg gtgtagtaca gacgcagata ttcatcaacg
    25021 ttgaacggta cgccctgcaa caggttaccg aacgctacac caattaccag cggcggaacg
    25081 aagctaccaa tgaagatgcc ccagtcccac atgttacgcc agcgggtttc ttcaatcttg
    25141 gagcggtagt caaaaccgac cggacggaag aacaaagacg ccagcacgag gatcatcgcc
    25201 acatagaagc cggagaacgc agcggcatag accatcggcc aggcagcaaa gagtgcgccg
    25261 cccgcggtga tcagccaaac ctggttaccg tcccagtgtg gtgcaatgga gttaatcata
    25321 attcgacgct cggtgtcgtt acgaccgagg aaacgggtga gcatgcccac ccccatgtcg
    25381 aaaccgtcag tgactgcaaa accaatcagc agaacgccaa ccagcagcca ccagataaaa
    25441 cgcaatactt cataatcgat catttgacga ctcctgtctt agcgtgccgg ctgagtagtc
    25501 gtggaagact gctcaaagtg atagcgaccg gttttcaggc tgcttgggcc gaggcgtgca
    25561 aacttgaaca ttaagaacaa ttctgccacc aggaacaggg tatacaggcc gcaaatcagc
    25621 accattgaga agatgagatc gcctgcggtc agtgacgaat tcgccacagc tgtcggcagc
    25681 acttcaccga tagcccacgg ttggcggcca tattcagcca cgaaccagcc cgcttctaca
    25741 gcaatccacg gcagcggaat accgtacagc gcggcgcgca gaagccattt tttctcgcca
    25801 atgcggttgc ggatgacact ccagaaagag agcgcgatga ttgccagcag caggaagcca
    25861 cacgccacca tgatacggaa cgcaaagtac agcggcgcta cacgcgggat ggagtctttg
    25921 gttgcctgtt gaatctgcgc ttcagtcgca tcagccacgt ttggcgtata gcgtttcagc
    25981 agcagaccgt aaccgaggtc tttcttcatg ctattgaact ggtcacgaac cgtctggtcg
    26041 gtagaaccag aacgcagttg ttcgagcaga gagtacgcct tcatcccgtt acgaatgcgt
    26101 tcttcatgct gcaccatcag ctctttcagg ccgataaccg gggtatccac ggaacgcgtt
    26161 gcaatgatgc ccagtgcgta agggatctga atcgcaaatt tgttcgtctc ctcttcctga
    26221 tcaggaatgc cgaacaaagt aaaggcagca ggcgcaggtt gcgtttccca ctcggcttca
    26281 atagcagcca gtttggtttt ctgcacgtcg cccatttcgt agccggattc atcacccaga
    26341 acaataacag acagaacagc agccataccg aagctggcag cgatagcaaa ggagcgttta
    26401 gcgaaggcga agtcacgacc tttcagcata taccatgcgc tgataccgag gatgaacatc
    26461 gcgccagtca cataaccaga cgctacagtg tgaacgaatt tcacctgagc aaccgggtta
    26521 agcaccagct cggagaagct caccatctcc atacgcatag tttcaaagtt gaaatcggac
    26581 gcgattgggt tttgcatcca gccgttcgca accagaatcc acagtgcgga caggtttgaa
    26641 ccgagcgcca ccagccaggt gacacacata tgctgaactt tacccagacg atcccaaccg
    26701 aagaagaaca gacctacaaa ggtggattcg aggaagaagg ccatcagacc ttcgattgcc
    26761 agcggcgcac cgaagatatc ccctacatag tgggaatagt aagaccagtt agtcccgaac
    26821 tggaactcca tggtcagacc ggtagccaca cccagagcga agttgatacc aaacaacttg
    26881 ccccagaact tggtcatatc tttataaatc tgtttgccgg agaggacgta gaccgtttcc
    26941 ataatggcca ggaggaacgc cataccgagc gtcagtggca caaaaaggaa gtggtacatc
    27001 gcggtcaagg caaactgtaa gcgcgacagt tcgactatat ctaacatcat gactccttgc
    27061 tcatcgcatg aagactccga gagtgaaccc cgttagaaag ggtcacacgc atgccccaat
    27121 agaaatttgt ttgaacactt tcatcatacc atgtgaatgc acacaaaaac ggcgtaaaga
    27181 aaggttgcaa acatgttaat aaaaactcaa attgatccca cgtatatatt acgccgcaaa
    27241 atccttacaa taaacaggtt tttgttgaag caaatttgca tttttcgacg gtgatcaatt
    27301 tatagctaaa ttgccgcctt tcagccaatt tgatcgagaa caatttatct ctttttgatg
    27361 cccatttcca agacttatac attgataaat atcaatttct acaaattatg ttaatcatgt
    27421 ttcaacaagg taactttagt gatatttatg tttaaaatgt gtgaattata agactgaaat
    27481 tagtttcggt tggcaattaa agataaaaag agacgaagca aaaacagcac ggttgacctt
    27541 aattcttttt ttggtcactg gctaatacga cgcagagttt caggattatt aataaaggca
    27601 taaatctccc tcccgccgat attaacattt aattaactta taattatatt ataattaact
    27661 cacccgtggt tattattttg ttaaaaataa agccacatat acgtagcttg agagtgaaga
    27721 taaacacaaa attacctgat gcgctacgct tgccagtgtc tacatcatct ctgcaatata
    27781 ttgaatttgc ctgcttttgt agaccgaata aagcgttcat gccgtatccg gcatgaacaa
    27841 agcgcccttt gtcaacaatc tggccgcgca tatgtggctt cggtttgatt gctattcagg
    27901 caagccggta actgaacgtc cgtgactggc cgggtaaaaa aggccccgat agatttgaac
    27961 cttgattttc ctcggtggta atgtgttcat ccatcatcgt ttctgagcaa gaaatcacct
    28021 cgcgactgaa agcaacagtc gcatcacagg ttgctgattc agccggatta aacagccgta
    28081 aaatcacttc ttgtcggtct tcagctttct taagtgcgct tatcaggcat cccactgggg
    28141 gcattttcaa caaactataa ctttccggca cgttgaatcc ggctttgttg agcttcatcg
    28201 catcccatgg gattttgttg taacactgta ctggagtcag ccatgctcgc gcctgctgag
    28261 ctacaccagc ggcggttggc gtaccggtat aactcaataa acttaggcga caagaaagca
    28321 gaccacgtag ttgtgagtcc gggactggca ttttaattcc cgaaggccgc ctaggcctta
    28381 aaagcagatc ttctttcccc agtaagccca cgccgcgcag caacgtaatg gcaaaggttt
    28441 tcttctcttc accgatgact tcaaattcac gtagcccttc gctaaagaca gccatgccgt
    28501 tacgcccttc ctgtaaggct gcatagttga gcatattcca taccggaacc ggcgcttctt
    28561 tccagctttc ttgctgccag ttgttcattg cactgtcgtt cacggggcgc gttagcgaac
    28621 caaactgggt atctgccaga acactatcgg tgttaaaaga tgtagggatc aggacgcgaa
    28681 ggcgatgatc gtcagcctgg ttatcaaggt tgatatccac atcaatgcgc ctgttgttat
    28741 gactaagagt gacaaccaac tcgacatcta ctctgccagt ggattgtctt gcgctgcgtt
    28801 gttgcagatt acgcggcacc gccatttcat agcggataac agccctgctc tgccaggcct
    28861 catgaataat ctcgcattgc ggtttcgcat tcgccgaagt aattacccac tcttcttttg
    28921 cgggtgaata gtcatattca tcaccatcat cagagctttc ttcaatttgc aacacccggt
    28981 catagcgcac accgctatct ttatctacca gttgtagcgt tccatcctca ttgagcgcaa
    29041 tttgccagaa agcattttcc agtatccctt cagcgtcact ttttgccgca atcacgttgc
    29101 caggctgatt cgcttcgata taaagcgtgc gatagcccat agaagggaca atctggttga
    29161 tctgtatatc aaactccata aagggatcgt aattaccgta atgaactatt tgccgatcga
    29221 ttaggcctgg atcgatctca cgggcatggc gaataaaata cggtacaggc tgaccgcgat
    29281 cgtctcgtag attaaactgg ctggcgcgca gccgcacagt ggtgttgatc acttcttccc
    29341 gcggccaggg catcaggtta aacaggacga gtttgtcggc gtcgctctgc ggcatgttgt
    29401 cggcgatttt acgcatatag aaacgcagca gattatccgc catgtcttca gccagttcga
    29461 agcgggcgac gatttcgcga tgaactttgt cacgacagca gcagccgatg ctgtcgtggg
    29521 catgattttt taagatctct ttccacattt tctccagcaa gccgtggtgg tatccaaaac
    29581 ccaacgtcct ggccagcgtt gccagcggtt ccagcagatt aacaatctta ttttcaatac
    29641 gtgcgtgggc aactttgata tccatgcgcg tggaaccgat ggtgcgatgc acgcgcatat
    29701 atttgccatc aataaattcc cctttcaggg ttgccagatt atctcgctgc gcttcgatct
    29761 tctcaaatac ctcttcaaag cggctcatca caaactttcg ttgaggataa atttcgcgca
    29821 gtttctccat cacttcgaag atattttgct gcaatggcat ctgatcatgc ccattcggca
    29881 gcaaaatctc tttggttaca gacgcttttt ccagcacgtc aaaataactg tcgaggcgtt
    29941 tgcgtaatcc gttttcgtcc gcaggtaagt acttaccgat ggcataaccc aacggcatca
    30001 cctgcgccgt cacttcgcta ccgtcactgc tttgccacaa aaactcggtt ttatccgtac
    30061 cgtggcgctc agaacatcca cgccagaaca tggtgcaggt aatgccaaat ccattgtaga
    30121 tatgcggcag ttgcccggac atgccaaagg aatcgggtaa ataacctatt ttcatcggct
    30181 cgccaaacgc gaggcagtca cgcattccgt acatcagatt acggacgatg gattccgcag
    30241 aaacaatcgt ggtatcggtc tgggtatacc aggggccgat aatcaacttg ccggcttcta
    30301 ccagtttctt caagcggtct ttgttttccg gtttcaccgc gaaataatct tcgaggatcg
    30361 ccgtttgccc gtcgagtacg taatatttgt attcgttgtc ctgttccagt cggcacagga
    30421 tctcttccat attattgacc agcagaatgc gtgactcttc ggtggtgaaa taccactccc
    30481 gatcccagtg catatgcggg gtgatgtgaa cgcgagatac cgctttcatc gtcgtttcct
    30541 ttttgtttat ggcattacgc catcagtcag atagttgcca tgcttaaccg cgtgacgccg
    30601 ccagatcagg agaattgcag tcgagattgc agcccccacc aatgccgcac caaaccagcc
    30661 aattgccgcc ataacaccgc ccgcgccatt atcatgaagt aaaaagagcg agaaaatgcc
    30721 agcaccgggt gtcgaaaggc cgatattcat cgcaccgaca atagcgcccg ttaccataga
    30781 gcccagcaca aacgaaccaa taacccgcag cggatcttca atcgccatcg ggatcgcccc
    30841 ttcggtaata cctgccagcc ctaacagcca ggtggatttc ccggtctcaa tttcaaactc
    30901 tttaaacagg cgcggtgcga gcatcgtgga agcggttacg gtaaatgccg aaaccatttt
    30961 gacggaggcg aaaatggcat acgggccgta aacgccgttc gccattgcgc ccaggcagaa
    31021 tgcataagcg gctttattca ctggccctcc aaggtcaaag gaacacataa aaccgagaat
    31081 ggcacccagc aacagcgcgt tacttcctga cagaccgttc agccaggcgg taagcgagtt
    31141 attgatccag gcgacaggtt cccccaccac aaacagcatc agactgcccg ctcccaatgt
    31201 accgagcacc gggtagagat aaaaagtcag gaagccattg aatttactgc taagacgcaa
    31261 gtgatttttc atccagcgta tcaagtaacc ggctatcaat ccgccaacga ccgcgccgag
    31321 aaacccggag ccgatcatgt tggcggcaag tccagccgca aagcctggcg ctaacgccgg
    31381 tttatctgcc agcgaatagg cggtataggc tgccagcacc ggtaccatca aaattccgag
    31441 cagcccgccg cccagtttgc ggtacatcca cagccaggag ttttcttcat taaacagatc
    31501 ttgtagcccg aagatttgcg acagtaatac cgcgaccgcc agcaccgtcc cccccgcgac
    31561 aatcaacggt acggcaaaag agatcccgct caacagtgcc tgtttcagct ccgttttgac
    31621 gcttttcacc ggttgcgtat cttgctgtaa ggtacgcgtc tcatcgccac gctcgagggt
    31681 aagcgattgt tggatcaacg cttctgcatg gcgaatcggc tcggcaacag gcacagaaag
    31741 cgcaggaatg ccgttaaaac gctcactctc cttgatggcg acttcagccg caaaaataca
    31801 ggcagttgca ctattgagtt gatccgccgt taaacgccct tcaataccgt tagcaccttg
    31861 cttttcaacg tagacattca cgccgagttt gcgtccggct ttttccagat actccgcagc
    31921 catgtaggtg tgagcaatac ccgccgggca ggccgttacg caaacgatag ttggcgcgtt
    31981 ggaaaaagag gcagaaggtt gcgtgcctcc cttgtcatcc agcgccgaga gcaattcatc
    32041 aggcgtcgtc gccgactgta tacgcgcccg aatctcatca tccgccaggc gcgtggtcag
    32101 cgctgtcagc agttgcatgt gcgtagtacc cgcttcattg aggggaatcg ccagcaacac
    32161 cactaaatca accgcttccg ggccatccac gccttcccac tgaagcggct cgctgagtgt
    32221 ggcgaccgca aacgccgctt cttttaccgc agcagttttg ccatgcggca cagccaaccc
    32281 ttcacttaag gcggtcgggc caaggctttc acggcgatac acttctttca gaaattgctc
    32341 agtactggaa attttcccca gagcagcaag acgttgagtc aacgcgtgaa tggcctcttc
    32401 acggctggta aagcgcgcat tcagacacaa cgcatcgcgg tgggtcagag tcgtcaggtt
    32461 catcatgatc ctcacttgat cgtttttata gtcgcgccag tgtgcccgac aaaacaatac
    32521 cattgcgtaa tcaatttcac ttgattgaaa ttatttgcat tttatttgta ttgtttacag
    32581 gatgagttta tcgggcataa tggctaaatt cgtattaatg agatgctaaa taatgggaca
    32641 caaaccctta taccgacaga ttgctgatcg cattcgtgag caaattgcgc gtggcgagct
    32701 taaacctggc gatgcgttac ctaccgaatc ggcattacaa acagagtttg gcgtcagccg
    32761 ggttacggtg cgtcaggctt tgcgccagtt agtcgagcaa cagatcctcg aaagcattca
    32821 gggcagcggg acttacgtca aagaagagcg ggtcaattac gatatttttc agttaaccag
    32881 ttttgatgaa aaactgtcgg accgtcacgt cgatacacac agtgaagttc tgatatttga
    32941 agtgattccg gctgacgatt ttcttcagca acagctacaa atcaccgtgc aggatcgtgt
    33001 ttggcatgtg aagcgcgtgc gctatcgcaa gcaaaagcca atggcgctgg aagaaacctg
    33061 gatgccgctt gcgttgttcc cggatctcac ctggcaggtc atggaaaatt cgaaatatca
    33121 ctttattgaa gaagtgaaga agatggttat cgatcgtagc gaacaggaaa tcattcctct
    33181 gatgccaacc gaagagatga gccgtctgct taatatcagc cagacaaagc cgattcttga
    33241 aaaagtatcg cgtggatatt tagttgatgg tcgggtgttt gaatatagcc gcaacgcttt
    33301 taataccgat gactataaat ttaccctgat agcgcagcga aaatcatcgc gataagcaca
    33361 aaaaggccat catacgatgg ccaaccatgt cgaaaccgac agcccttcgc cattcctgta
    33421 gagggaagcg gcgagggcta tttcttatta cagatattta tttcagaaca gttttcagtg
    33481 cttcaccgat atccgccagg ctgcgaacgg ttttcacgcc tgcggcttcc agagcagcga
    33541 atttctcatc cgcagtccct ttcccaccgg caatgatcgc acccgcgtgg cccatacgtt
    33601 tgcctttcgg cgcagtcaca ccagcgatgt aacccacaac tggcttggta acatgctctt
    33661 tgatgtacgc tgccgcttct tcttcagcgc taccgccgat ctcaccgatc atcacgatcg
    33721 cttcggtctg cggatctttt tcgaacattt cgagaatgtc gataaagtta gagccaggga
    33781 tcgggtcacc gccgataccg acacaggtcg actgaccgaa accgtaatcc gtggtctgtt
    33841 taaccgcttc ataggtcagt gtaccggaac gggaaacgat acccacttta cccggtttgt
    33901 gaatgtgacc cggctggata ccaattttgc attcacccgg ggtgataacg cctgggcagt
    33961 tcgggccgat catacgaacg cctgcttcgt ccagtttcac tttcacggtc agcatatcca
    34021 gcgtcgggat gccttcagtg atggtgataa tcagtttgat gcctgcatcg atagcttcca
    34081 gaatggagtc tttgcagaac ggtgctggta cgtaaataac agacgcggaa gcgccagtag
    34141 cagcaacagc ttcacgcacg gtgttgaaca ccggcaggcc gaggtgggta gtgccgcctt
    34201 tacctggagt tacgccacca accattttag tgccgtatgc aatggcctgt tctgagtgga
    34261 aagtcccctg gctaccggta aagccctggc agataacctt ggtgttttta tcaattaaaa
    34321 tggacattat ttcccctcca ctgcggcaac aacctgctga gctgcatccg tcagaccttt
    34381 tgctgcaata atattcaggc cgctgtcagc cagtttcttc gcgccgagtt cggcgttgtt
    34441 accttccaga cgtaccacga ccggtacgtt aacacccact tccgctaccg cgccgatgat
    34501 accgtcagcg atcaggtcgc aacgaacgat accgccgaag atgttaacca gaacggcttt
    34561 cactttgtcg tcagagagga tgattttgaa cgcttcggtt acacgttctt tggttgcgcc
    34621 gccgccaacg tccaggaagt tagccggttc gccgccgtgc agtttaacta tgtccatcgt
    34681 acccatcgcc agacctgcgc cgttaaccat acaaccgatg ttaccgtcca gcgcaacgta
    34741 gttcagttcc cactgtgcag cctgtgcttc acgcggatct tcctgcgact ggtcacgcat
    34801 ttcgcgcaga tcaggctggc ggaacagggc gttgccatca gcgcccagtt tgccgtcgag
    34861 gcaaatcaga tcgccctgtt tggtgatgac cagcgggttg atttcgatca gcgccaggtc
    34921 gcgctccagg aaaatggtcg ccaggcccat gaagattttg gtgaactgct gaaccagttt
    34981 accttccaga cccagtttga acgccagctc gcgtccctga tacggcatcg ggccagtcag
    35041 cggatcaagc gcaactttat ggatcaggtg cggagtttct tccgccactt tttcgatttc
    35101 cacgccgcct tcggtggagg ccataaagac cacgcgacgg gaactacggt ctacaacggc
    35161 accgagatac agctctttag cgatatcggt cgccgcttca accagaatct ggttaaccgg
    35221 ttggccattg gcatctgttt gatacgttac cagacgcttg cccagccagt tttctgcaaa
    35281 agcacggatg tcttctttgc tgtttacaac tttcacaccg cccgctttac cgcggccacc
    35341 agcgtgaacc tgacatttca ctacccacgg accggcaccg atttttgaag cggcttcttc
    35401 tgcttcgcgc ggagtagtac aggcataacc caccggtgct ggtaagccat agcgggcaaa
    35461 cagttgtttt gcctgatatt catgtaagtt catgtgttct gtccatcctt cagtaatcgt
    35521 tatcttttaa accgtagacc gacaccctat ccacgtcggt ctacagtgca ggtgaagctt
    35581 aaactactac acgtccagca gcagacgcgt cggctcttcc agtaactctt tgatcgttac
    35641 caggaagccc acggattcgc gaccatcgat cagacggtga tcgtaggaca gcgccaggta
    35701 catcatcggc aggatctcaa cctgaccatt caccgccatc ggacgatctt tgatagcgtg
    35761 catacccaga attgcgctct gcggcgggtt gatgatcggc gtagacatca gggaaccgaa
    35821 cacaccaccg ttggtgatgg tgaagttacc accggtcaga tcttcaaccg tcagcttgcc
    35881 atcacggcct ttgactgcca gctctttgat tttcttctcg atgtctgcca tgccgagggt
    35941 atcgacatca cgcagaaccg gcgtcaccag gccgcgcggc gtagaaaccg ccatgctgac
    36001 gtcgaaatag ttgtggtaaa ccacgtcatc gccgtcgata gacgcgttca cttccgggta
    36061 acgtttcagg gcttcaacca ccgctttcac gtagaaggac ataaagccca gacggatgcc
    36121 gtggcgtttt tcaaacgctt caccgtactg cttacgcaga tccataatcg gcttcatgtt
    36181 gacttcgttg aacgtggtca gcatggcggt ggagtttttc gcttccagca gacgctctgc
    36241 cacacgctta cgcaggcgag tcatcgggac acgtttttca ctacgtgcag ccagagccgg
    36301 ttgcgccgcc ggagcagccg ctgccggagc agactctttc gccggggctt tcgccagatg
    36361 tttttccaca tcttcacgag tcagacgacc acccacaccg gtgcctttaa tggcgctggc
    36421 gtcgagattg tgttcagcca gcaggcgacg gatcgccggg cttaacgcat cgttgttttg
    36481 ctcttccaga gacgcctgct ggcgttgcgc cggagtggac gctttctctt cagatttggc
    36541 gctggtttct ttaccggcgc tgttgccttc acgcaggcga ccaaggatct gacgagacgt
    36601 taccgttgta ccttcatctt ccagaaccgc atccagaatg ccgtctgctg atgccggtac
    36661 ttccagtacc actttgtcag tttcgatttc taccagcact tcatcacgta cgactgcgtc
    36721 gccgggtttt ttatgccagg ttgcgacggt ggcatcggct acggattcag gcaggtcagg
    36781 gaccagaata tctacgctac tcattgtgta tcctttattt attcgacgtt cagcgcgtca
    36841 ttaaccagat cttgttgctg tttctggtga acggacatat accctaccgc cggagaggcg
    36901 gaggccgggc ggcctgcata acgcagagaa gccccaaacg gaatcacttc acggaaatga
    36961 tgctggctgc agtaccatgc gccctggttg agcggctctt cctggcacca gacaaaatcc
    37021 ttgacgtgag caaactgctg caacacttcc tgcatcgctt tatgcgggaa cgggtagagt
    37081 tgctcgatac gcacaatggc gacatcgtgt tgattgttct tacgacgctg ttccagcagg
    37141 tcgtaataaa ccttaccaga acacatcact acgcgcttca cgcccttcgg atcaagctcg
    37201 tcgatttcac cgatggctgg caggaaggtg ccgttcgcca gttcttcgag gctggaaacc
    37261 gccagcggat gacgcagcag ggatttcggc gacatcacga ccagcggacg acgcatcccg
    37321 cgcagcgcct gacgacgcag catgtggtaa acctgtgccg gggtagacgg tacgcaaacc
    37381 tgcatgtttt gctcagcaca aagttgcaga taacgttcca gacgcgcgga ggagtgctcc
    37441 ggcccctgcc cttcgtaacc gtgcggcagc aacatcacca gaccacacat ccggccccat
    37501 ttctgctcgc cagaggagat gaactggtcg ataaccacct gcgcaccgtt ggcgaagtca
    37561 ccgaactgcg cttcccagat ggtcagggtg cgtggttctg cggtggcata accatattca
    37621 aacgccagca cggcttcttc tgacagtacg gagtcccaga cacggaacgc gccctgaccg
    37681 ttatggatgt gttgcagcgg cgtgtaagtg gaaccgttag actggttgtg gatcaccgcg
    37741 tggcggtgga agaaggtacc gcgaccggag tcttcacccg acaggcgaac cggaatgcct
    37801 tcatcaacca gcgtggcgta agcgaggttt tccgcaccgc cccagtcgaa cagtttctca
    37861 ccggctgcca tcgcctggcg atcgccataa atcttggcaa cgcgagactg catttcaact
    37921 gcttctggta ccgtactgat acgtttagcc agttcctgca ggcgcttcat ctcaactttg
    37981 ttcgggtact cttcgtccca ttcgtggttg aggtacggcg accaggtgaa agagtgcatg
    38041 ttcatcggac gccactctgc cactacgcaa tcgccagcat ccagcgcatc gcggtacagg
    38101 ttaaccatct cggtggcatc ttccagcgtc gccacttttt cctgctccag cttgtcagcg
    38161 tagattttgc gcggtgtcgg atgttttttg attttctgat acatcagtgg ctgggttgcg
    38221 ctcggctcgt cggcttcgtt gtggccgtga cggcggtagc ataccaggtc gatgaagaca
    38281 tcacgtttaa aggtgttacg gaaatcgagc gccagacggg tcacaaaggc aacggcttcc
    38341 ggatcatccg cgttaacgtg gaaaatcggt gcctgaacca tcttaccgat atcagtacag
    38401 tacggtgtag aacgggcatc cagcgggtta gaggtggtga aaccaacctg gttgttgata
    38461 acgatacgta ccgtaccgcc aacttcataa ccacgcgctt tcgacatgtt cagagtttcc
    38521 tgaaccacgc cctgcccggt cactgcggcg tcaccgtgga tggtgattgg cagcactttg
    38581 ttgctgttcg gctcatcaag tctgtccaga cgggcacgaa cagagccgat aacgaccggg
    38641 cttacaatct caaggtgaga cgggttaaac gccagcgcca ggtgcaccag gccgccatcg
    38701 gtctggaagt cagacgagaa gcccatgtgg tatttcacgt cgcctgtgcc gaggtgttct
    38761 ttatgtttac cggcgaactc gtcgaacaag tcttgcggtt ttttacccag cacgttcacc
    38821 agcacgttca gacgaccacg gtgcgccatc ccgagaacca cttcgcgggt gccgctgttg
    38881 ccagcgtggc ggatcatctc tttaagcatc gggattaacg cgtcaccgcc ttccagcgag
    38941 aagcgttttg cgccagggaa ttttgcgccg aggtaacgct caaggccttc agcggcggtc
    39001 agttcgctta agaagcgttt tttctcttcg ctattgaaag tcgcgcgacc agactcgata
    39061 cgctgttgga tccagcgttt ttcttcggtg ctggtgatgt gcatatactc ggcaccaatc
    39121 gggccgcagt aggtttgctt gagggcggaa ataagctcgc cgagtttcat ggtttctttg
    39181 ccgctggcaa atgaaccgac gttgaaggtt tcctggaagt ctgcttcggt cagatcgtgg
    39241 aaagacggat ccagatcggc cactttatct tgctgccaca gtcccagcgg atctagattc
    39301 gcatgctggt gaccacggaa gcggtatgcg ttaatgagct gcaggacttt aacctgcttc
    39361 acattggtgt cagggtcgga gatcgttgaa gagtaacgtg aagcgtcttt cgccaggcgg
    39421 cggaaatatt cacgcgtttg agagtggaat tgatccggtt tgactcccgt accaggtaac
    39481 tgctggaacg tcgaacgcca gttagcgtca accgagtcag gatcggttaa gaagtcttca
    39541 tagagctgtt ctatccagct ctggtttgcg ccagagaggt aagaagagtc caaccaggct
    39601 ttcaaagcgc tgttctgcat cgtgatccct taagcatctt ttttatgctt acttcgccgt
    39661 ggatactacc acgcacagtg atgtgcgtgc cggggttcac ttgcgagctc ttgtctacgt
    39721 agtggctcgc gaaggaacct ttaaaaactg tcttagtgtc aggggcagtt tttaaagatt
    39781 tcctgcattt ctttgttacg cctgatgcgc tgcgcttatc aggcctgcgg gtaatgactt
    39841 gtaggccgga taaggcgttc acgccgcatc cggcactggt tgcctgatgc aacgcttgcg
    39901 cgtcttatca ggcctacagt ttacgcatta cgttgcaaca acatcgactt gatatggccg
    39961 atggcgcgcg tcgggttcag ccccttcgga catacactga cgcagttcat gatgctgtga
    40021 cagcggaata cgctgaatgc atcgctcaaa ccgtcgaggc ggctgtcagt ctcggtatca
    40081 cggctatcga tcaggaaacg atatgccgct aacaagcctg ccgggccgat aaacttatcg
    40141 ggattccacc agaaagacgg acaagaggtt gaacaacatg cgcagagaat acattcatac
    40201 agcccgtcga gtttttcgcg ctgctctggc atctgtaaat gctcgcgagc tggcggattt
    40261 tgtccattat tcaacaggta aggcttaatt ttctcatatt gcgcatagaa ttgtcccatg
    40321 tctaccacca aatcgcggat caccggtaaa cctggcagcg ggcgaatcac aatcttcttg
    40381 cccggctggt tgagtgccga aatcggggta atacaggcca gaccattctt gccgttcatg
    40441 ttcagaccgt cggaaccgca cacaccttca cggcaggagc ggcggaacga caggctggga
    40501 tctttctctt tcagctggat aagcgcatcc agcagcatca tgtcgcgacc ttcttccgct
    40561 tccagggtgt aatcctgcat acgcggagca tcatcaacat ccgggttata gcgataaatt
    40621 gaaaactcga gtctcatttt cctgtctccg cattagtaag tacgaatctt cggcgggaat
    40681 gccgggcgca gtttcggttc catgttgacg cttcggcgcg tcatggattc cgactcaggc
    40741 agatacaggg agtggcacag ccagttttca tcatcacgat ccgggaagtc gaagcggcta
    40801 tgtgcgccac ggctttcggt acggaagttg gcagaaacag ccgttgcata cgccgtttcc
    40861 atcaggttat ccagttccag gcactcaacg cgctgggtgt tgaactcgct ggaagtgtca
    40921 tccagacggg catttttcag acgctcgcgg atcactttca actgctcaag ccctttcgcc
    40981 atcgcatcac cttcacggaa gaccgagaag ttatgctgca tacattcttg cagcgcttta
    41041 cggatcgcca ccggatcttc accgttacga ttattgttcc accggttcag gcgatccaga
    41101 gacgcttcaa catcagactc gctggcatcg tgcagtgcgc cctgctcggc gatagactct
    41161 tgcagatgca gacctgccgc gcgaccaaag accaccaggt ccagcagcga gttgccgccc
    41221 agacggttag cgccgtgtac cgatacacaa gcgatttcac caacggcaaa cagtcccgga
    41281 acaaccacat cttcgccttt ctcattcaca gtcagcgcct gaccggtaac tttggtcgga
    41341 ataccgccca tcatgtagtg acaggttggg ataaccggaa tcggctcttt caccggatcg
    41401 acgtgagcga aggtacggga aagctccagg atacccggca gacgggattc gagaacttct
    41461 ttacccaggt gatcgagttt cagtttcgcg tgtggccccc acggaccatc acagccgcga
    41521 ccttcacgga tttcaatcat gatggaacgc gccaccacat cacggcccgc caggtctttg
    41581 gcgttcggcg cataacgctc cataaagcgt tcgccatgtt tgttcagcag ataaccgcct
    41641 tcaccacggc aaccttcggt gaccagtacg cccgcaccgg caatgccggt cgggtggaat
    41701 tgccacattt ccatatcctg caccggtacg cctgcgcgga tagccatgcc gacaccgtcg
    41761 ccagtgttaa tgtgggcgtt agtggtggac tgataaatac gccctgctcc gccagtcgcc
    41821 agcacggtag cgcgggcttt gaaataaacc acttcaccgg tttcgatgca cagtgcggta
    41881 caacccacca ccgcgccatc ctggtttttc accagatcca gcgcatacca ctcggagaaa
    41941 atggtggtgt ggtttttcag gttctgctga taaagcgtgt gcaacagtgc gtgaccggta
    42001 cggtcagcag ccgccgcagt gcgtgccgcc tgctcgccgc cgaagttttt cgactgaccg
    42061 ccaaacggac gttgatagat acgaccatca tcgagacgcg agaacggcag gcccatgtgt
    42121 tcgagttcca gaatcgcttc cggcccggtt ttacacatat attcaatcgc gtcctggtca
    42181 ccgatatagt ccgacccttt tacggtgtcg tacatatgcc attcccagtt atcttcatgg
    42241 gtattaccca gcgcaacggt aatgccgcct tgcgcagaaa cggtatggga acgggtcggg
    42301 aagactttag agagcagggc acaggtctgg ccgctctggg aaatttgcag cgccgcgcgc
    42361 atacctgcgc caccggcacc aatcacaact gcatcaaatt ctctgactgg caatttcatc
    42421 acacacccca caccacaacg aatccataaa tcacgtaaac caccagtgca acgacaatca
    42481 ccagttgcag catcaggcgc aaggccagcg gtttaacgta gtcggtcaac acctgccaca
    42541 tgccgatcca ggcatgaatc aagatagaaa acagcgccag cagggtgaac actttggtga
    42601 acgcagaggc gaagaaaccg atccagactt catatgtcag ttcgccactg gtggcgaaaa
    42661 aaccgaccat ataaatgatg tagagcgtca ggacgatagc ggtagcgcga acgaggatga
    42721 aatcatgtac gccattgcgt cctaatgcgg aggcgttgct taccatacga ggactcctgc
    42781 gagaagtgaa agcacgacag taataacaaa ggagattttg gcggagcgtt tacccgcttc
    42841 gaatgtttct tccagatagc caaaatccat catcatgtgg cgaataccta cgacgacgtg
    42901 atacgccaga gcggtaagga tgccccacat gataaatttg acgaagaagc tgcccataat
    42961 cgcggaagct tgctcgaaac cttcagggga agagaggctg gtacccagaa gccactgcag
    43021 gatgcccact gcaacaaagg tgatcacacc ggaaacgcga tggagaatgg acgctatcgc
    43081 cgtgatgggg aaccggatgg tctgtaggtc cagattaaca ggtctttgtt ttttcacatt
    43141 tcttatcatg aataacgccc acatgctgtt cttattattc cctggggact acgggcacag
    43201 aggttaactt tctgttacct ggagacgtcg ggatttcctt cctccggtct gcttgcgggt
    43261 cagacagcgt cctttccata actgcgcgtc atgcaaaaca ctgcttccag atgcgaaaac
    43321 gacacgttac aacgctgggt ggctcgggat tgcagggtgt tccggagacc tggcggcagt
    43381 ataggctgtt cacaaaatca ttacaattaa cctacatata gtttgtcggg ttttatcctg
    43441 aacagtgatc caggtcacga taacaacatt tatttaattt ttaatcatct aatttgacaa
    43501 tcattcaaca aagttgttac aaacattacc aggaaaagca tataatgcgt aaaagttatg
    43561 aagtcggtat ttcacctaag attaacttat gtaacagtgt ggaagtattg accaattcat
    43621 tcgggacagt tattagtggt agacaagttt aataattcgg attgctaagt acttgattcg
    43681 ccatttattc gtcatcaatg gattctttac ctgcaagcgc ccagagctct gtacccaggt
    43741 tttcccctct ttcacagagc ggcgagccaa ataacaaacg ggtaaagcca ggttgatgtg
    43801 cgaaggcaaa tttaagttcc ggcagtctta cgcaataagg cgctaaggag accttaaatg
    43861 gctgatacaa aagcaaaact caacctcaac ggggacacag ctgttgaact ggatgtgctg
    43921 aaaggcacgc tgggtcaaga tgttattgat atccgtactc tcggttcaaa aggtgtgttc
    43981 acctttgacc caggcttcac ttcaaccgca tcctgcgaat ctaaaattac ttttattgat
    44041 ggtgatgaag gtattttgct gcaccgcggt ttcccgatcg atcagctggc gaccgattct
    44101 aactacctag aagtttgtta catcctgctg aatggtgaaa aaccgactca ggaacagtat
    44161 gacgaattta aaactacggt gacccgtcat accatgatcc acgagcagat tacccgtctg
    44221 ttccacgctt tccgtcgcga ctcacatcca atggcagtca tgtgtggtat taccggcgcg
    44281 ctggcggcgt tctatcacga ctcgctggat gttaacaatc ctcgtcatcg tgaaattgcc
    44341 gcgttccgcc tgctgtcgaa aatgccgacc atggccgcga tgtgttacaa gtattccatt
    44401 ggtcagccat ttgtttatcc gcgcaacgat ctctcctatg ccggtaactt cctgaatatg
    44461 atgttctcca cgccgtgcga accgtatgaa gttaatccga ttctggaacg tgctatggac
    44521 cgtattctga tcctgcacgc tgaccatgaa cagaacgcct ctacctccac cgtgcgtacc
    44581 gctggctctt cgggggcgaa cccgtttgcc tgtatcgcag caggtattgc ttcactgtgg
    44641 ggacctgcgc acggtggtgc taacgaagcg gcgctgaaaa tgctggaaga aattagctcc
    44701 gttaaacaca ttccggaatt tgttcgtcgt gcgaaagata aaaatgattc tttccgcctg
    44761 atgggcttcg gtcaccgcgt gtacaaaaat tacgacccgc gcgccaccgt aatgcgtgaa
    44821 acctgccatg aagttctgaa agagctgggc accaaagatg acctgctgga agtggctatg
    44881 gagctggaaa acatcgcgct gaacgacccg tactttatcg agaagaaact gtacccgaac
    44941 gtcgatttct actctggtat catcctgaaa gcgatgggta ttccgtcttc catgttcacc
    45001 gtcattttcg caatggcacg taccgttggc tggatcgccc actggagcga aatgcacagt
    45061 tacggtatga agattgcccg tccgcgtcag ctgtatacag gatatgaaaa acgcgacttt
    45121 aaaagcgata tcaagcgtta atggttgatt gctaagttgt aaatatttta acccgccgtt
    45181 caaatggcgg gttaattttt atatgcctaa acacaaaaaa ttgtaaaaat aaaatccact
    45241 atcggacatt catatattct aaaaaaaaat acatcggtat aaattataaa caaatagatg
    45301 atttttaatc atctatcttc ctctcagtta catgaataaa ccaccaaata tagcgcaccc
    45361 ctataattcc caatacttta ttcatcaaac atttaattca taccatcaat taattacttt
    45421 tacatttaat aaaattaata atatttttaa gaaaaactaa aaacattcga caatcaaata
    45481 agatattctt gcaccgatat acttttttct tttctgttaa aggactcagt tatgtttaaa
    45541 ggacaaaaaa cattggccgc actggccgta tctctgctgt tcacagcacc tgtttatgcg
    45601 gctgatgaag gatccggtga aattcacttt aaaggtgaag ttattgaagc accgtgtgaa
    45661 atacatcagg atgatattga taaagaggtt gaactcggtc aggtgaccac cagccacatt
    45721 aatcagtcac atcacagcga tgccgttgct gtcgacctgc tcttagtcaa ctgtgatctg
    45781 gaaaactcca gcaacggttc cggtggcaag atttctaaag ttgcagtcac ctttgatagc
    45841 tcagcgaaaa ccaccggcgc agatccgatt ctcaacaaca ccagcacggg tgaagccacc
    45901 ggcgtcggcg tacgtttaat gaataaagat caaagcaata tcgttttagg caccgctact
    45961 ccagatatcg acctggctcc gacctccagc gaacaaacgc tgaatttctt tgcctggatg
    46021 gaacaaattg atcaggctac acctgtaacg ccaggcgcag ttacggcgaa tgcaacctac
    46081 gtgctggatt ataaataagt attactttgg cggcgctatc gccgcctttc ataaaatatt
    46141 tatcaggtat ggacaccgtg aatatttatc gactctcttt tgtatcctgc ctggtcgtgg
    46201 cgatgccttg cgcattggcg gtcgaattca atctgaatgt tctcgataaa tcaatgcgcg
    46261 accgcattga tatttcatta ttaaaggaaa aaggagtcat tgctcccggt gagtattttg
    46321 ttagcgttgc ggtaaataat aaccaaatca gtaacgagca aaagattaac tggcacaaaa
    46381 atgacgataa aaccattccg tgcatcaatg atttactggt cgataaattt ggcttaaaac
    46441 ctgaagtccg tcagtcgtta ccattgataa atcagtgcgt cgattttagc tcccgacctg
    46501 aaatgctctt caatttcgat caagccaatc agcaactgaa tattagtatt ccgcaagcct
    46561 ggctggcgtg gcactcagaa aactggaccc caccctccac atggaaagaa ggtgtcgccg
    46621 gtgtcctgat ggattacaac ttgtttgcca gcagctaccg cccacaggat ggcagcagca
    46681 gcactaatct taacgcctat ggaacagccg gaatcaacac gggggcatgg cgcttacgca
    46741 gtgattatca gttgaatcat actgatagcg atgataacca tgaacagtca ggcgaaatat
    46801 cacgtaccta tctttttcgc ccgttaccgc aattaggttc aaaattaacc ctcggcgaaa
    46861 cggattttag tcccaatatt ttcgacggtt tttcttatac cggcgcggca ctggcaagtg
    46921 acgatcgaat gttgccatgg gaactacgcg gctacgcccc acaaattagc ggtattgcac
    46981 agaccaatgc cacggtgacg atcagtcaat caggccgcgt catttaccag aaaaaagtcc
    47041 caccaggccc atttatcatt gacgacctta atcagtctgt tcagggcaca ctggatgtca
    47101 aagtgacgga agaagatggt cgggtgaaca atttccaggt ttcagcagca tcgacgccct
    47161 tcctgactcg tcagggacag gttcgctata aactggccgc gggtcagcca cggccctcca
    47221 tgtcacatca aactgaaaat gaaacctttt ttagcaatga agtttcctgg gggatgctgt
    47281 caaacacctc gctgtacagc ggcctgctgc tttctggtga tgactaccat tctgccgcaa
    47341 tgggtattgg gcaaaatatg ctgtggcttg gtgcgctgtc gtttgatgtc acgtgggcca
    47401 gtagccattt tgatactcag caggacgagc ggggcttaag ctaccgtttt aattacagca
    47461 aacaagtgga tgccactaac agcacgattt ccctcgccgc ttatcgtttc tccgatcgtc
    47521 attttcacag ctacgccaac tatctggatc acaaatacaa cgacagcgat gcgcaggacg
    47581 aaaaacagac gatcagctta tctgtgggcc aaccgattac cccactaaac ctcaatcttt
    47641 acgccaacct gctacatcaa acctggtgga atgcagacgc ctccacgacc gccaacatca
    47701 cggcaggttt taatgttgat attggtgact ggagagatat ctctatttct acgtcattca
    47761 atacaaccca ttacgaagat aaagatcgcg acaaccagat ttacctgtcg atttcgctcc
    47821 ccttcggtaa cggtggtcgg gttggctatg acatgcaaaa cagtagccac agcaccacac
    47881 accgcatgtc gtggaacgat acgctggatg aacgtaatag ctggggcatg tctgccggac
    47941 tgcaatccga ccgtcctgac aatggagccc aggtgagcgg taactatcag cacctgagtt
    48001 cagcgggtga gtgggatatt tctggtacct atgccgccaa tgattacagt tccgtcagca
    48061 gcagctggag cggttctttc accgcaaccc aatatggtgc agcgtttcat cgccgcagct
    48121 ccaccaatga accacgcctg atggtcagca ccgatggcgt ggcagatatt ccggttcagg
    48181 gcaatctcga ctacaccaac cattttggca ttgcggtggt gccgttgatt tccagttacc
    48241 agccttccac cgtggcggtg aacatgaatg acttacccga cggcgtaaca gttgcagaaa
    48301 acgttatcaa agaaacgtgg attgaaggcg cgataggtta caaatcactg gcttcccgtt
    48361 ccggtaaaga cgttaacgtc atcattcgca acgccagcgg tcagtttcct cccctcggag
    48421 cggatatccg ccaggatgac agcggcatta gcgtggggat ggttggcgag gaaggacatg
    48481 cctggttaag cggagtcgct gaaaatcaaa agtttaccgt ggtctggggt gatagccagc
    48541 attgctcgct ccatcttcct gaacatatgg aagacaccgc aaatcgcctg attttacctt
    48601 gtcattaatt aaggaaatga aaatgacatt tatgaaagga ctaccccttt tgttgttggt
    48661 tgccagtctg tgcagccacg ctgcactaca acccgatcgc actcgtattg tgtttaacgc
    48721 taatgataaa gctaccagtc tgcgggtgga taatcgtagc gataaactcc cctacctcgc
    48781 ttattcctgg ctcgaaaatg aaaaagggga gaaaagcgat gatcttctgg tcgccctgcc
    48841 gccgatacaa cgtctggaac cgaaagcaac aacacaggta cggatagtaa agcaagcctc
    48901 gaccacaaaa ttaccgggcg atcgcgaaac gctatttttc tacaatatgc gtgaaattcc
    48961 tcccgccccg gaaaaaaaca gcgaccatgc cgttctgcaa gtcgccattc agagtcgtat
    49021 caaggtgttc tggcgaccgg ctgcattacg caagaaagca ggagaaaagg tcgaactgca
    49081 gttacaggtc agccagcagg gcaaccaact gacactgaaa aatcctaccg cgtattatct
    49141 gaccattgcc tacctgggac gaaatgaaaa aggcgttctc cccggcttca aaaccgtgat
    49201 ggttgcgcct tttagtacgg tcaacacaaa caccggaaat tatagcggca gccagtttta
    49261 tctcggttat atggatgatt acggcgcgtt acgcatgacg acgctcaact gcagcggaca
    49321 atgccattta caagccgtgg aggcgaagaa atgagtgctg gcaagggagt gttgctcgtc
    49381 atctgtctgt tatttctgtc gttgaagtct gcgcttgccc tgaactgcta ctttggttca
    49441 tccggtggct cggtagaaaa atcagaagcg attcaacctt ttgctgtacc aggcaatgcc
    49501 aaactcggcg ataagatctg ggaatctgac gatattaaaa taagatctgg gaatctgacg
    49561 atattaaaat tcccgtctat tgtgacaaca ataccaacgg caactttgaa agcgaacatg
    49621 tctacgtatg ggtaaacccc tacccggggg tgcaggatcg ttattatcaa ctcggagtaa
    49681 cttacaatgg cgttgattat gacgccaacc agggaaaaag ccgcatcgac actaaccagt
    49741 gtatcgacag taagaacatt gacatttata cccctgatgg gatggcagaa caaaatttgc
    49801 tcgggtgatc ccgctaatat tcacatgtcg cgaacctttc tcgctcgcat gcggttatac
    49861 gtcaaaatac gagaaatgcc gccgcatgat tatcaaagta cgcttagcga ctatatcgtc
    49921 gtgcaatttg acggtgccgg tagcgttaac gaagacccta ctgcccaaaa cctgaaatat
    49981 catattactg gtctggaaaa cattcgcgtg ctggattgca gcgtcaattt ttccatttca
    50041 ccggaaacac agatgattga ttttggtaaa tttaatttgc tggatattcg tcgccacaca
    50101 atgtcgaaaa cattcagtat taagacgaca aaatcacaaa atgaccaatg caccgacgga
    50161 tttaaagtca gttcctcctt ttataccgaa gaaacgttgg ttgaagaaga taaagcattg
    50221 ttgattggta atggtctgaa gctacgtttg ctggatgaaa acgcctcgcc ttataccttc
    50281 aataaatatg ctgaatacgc cgatttcacc agcgacatgt tggtctacga aaaaacctat
    50341 acagctgaac tttcgtccat tgcaggtacc cccatcgagg ctgggccctt cgatacagtg
    50401 gtgcttttta aaattaacta taactgattt gccatgatgc cctttgcagc cggaaagggc
    50461 atctctttca gaaaattgct gattttcccc ttttccttga ttgctgttat tttttcctag
    50521 cccggctttc tttatgctgt atcgatacac caaagagaaa ataatgaggg agcgaaggat
    50581 gccagttttg cagtggggaa tgttatgtgt gctatcactt ctcctttcta ttggtttcct
    50641 cgcggtacat ctcccggcgg cgctattact tgggccgatg atcgccggga tcatcttcag
    50701 tatgcgcgga attactctgc aacttccccg ctccgctttt ctcgccgcgc aggccattct
    50761 tggctgcatg attgcgcaaa acctcactgg ttcgattctc accacgctgg ccgccaactg
    50821 gccgatcgtg cttgctattt tactggtgac gctgctttcc agcgccatcg tgggttggtt
    50881 attggtgcgc tatagctcac tgcctggaaa taccggtgcc tggggttctt ctccaggcgg
    50941 cgcagcggca atggtcgcta tggcgcaaga ttacggcgca gatattcgcc tggtggcgtt
    51001 tatgcaatat ctgcgagtgc tgttcgtcgc gggggccgct gttctggtca cccgcatgat
    51061 gctgggtgat aacgctgaag cggttaacca gcaaatcgtc tggttcccgc cagtgagcat
    51121 taatctcctg cttacgattt tgctcgcagt cgttgccggt acggcgggat gcatgctgcg
    51181 tcttccttcc ggcacgatgc tcatcccgat gctggcgggc gcagtgctcc agtctggtca
    51241 gctcatcacc atcgaactac cggaatggct gctggcgatg gcgtatatgg caattggctg
    51301 gcggattggt cttggtttcg ataaacaaat cttactgcgg gcactacgcc cgctaccgca
    51361 aatcctgctg tcgatttttg ctctgctggc tatttgtgca ggtatggcgt ggggactgac
    51421 ccggtttatg catattgatt ttatgactgc ctacctcgcc accagtcccg gcgggcttga
    51481 taccgtggcg gtcatcgccg cagggagcaa tgccgatatg gcgcttatta tggcgatgca
    51541 aaccctgcgc ctgttcagta ttttgctgac ggggccagcc attgcgcggt ttatttcaac
    51601 ctatgcgccg aagcggtcgg cctagtgctg gcagcctgga caccagtaaa acgggcgcga
    51661 ggacaacgtg gttttctcaa tgatgccgcc acagcgttcg cacagttcgc catcacgatg
    51721 aaaaacctta aagcgaaaca gcgccccatg atgcttattc tcatccacct gcccccgcgt
    51781 agcgtaagaa aaccgaggaa tctccagtaa cgcgtgtgcg agtgcatcca gttgcgccgc
    51841 attgagatct ttcgctttat gatttccagt caacccaacc tgccagagga tctccacccg
    51901 caaataattg ccaagcccag ccagaaacgc ctgatcgagc agtaatcctg caaactgacg
    51961 gttacgaaag cgcggcgaca ataaccgttc ctttaccacc tccggcgtca gattcggatc
    52021 cagcacatcg ggaccaacgc gttgtaaaaa cggatgcgtg gtcagttgtt ccggcctcaa
    52081 catctcaata tccgaggcgc tataaagcag aatggttttg tcagccgttt gcagttttac
    52141 ccgcaatact cgcgtggtct gcggctcttc gccggtatca accacgcgcc agacgccgta
    52201 aagctgatta tggctgtaga gcgttaaacc gttgggaaaa tgagttaaca acgccttacc
    52261 acgcgtttcc acatgggtaa cgtgttgacc gataagttgt gattgataag tttttaactg
    52321 cgggaaggca aaccagacat cagttagtgg tttgcctttg atcgccgcct ccaggttatc
    52381 cgctgcacgg cggatctccg ggccttcagg catggtgata tccttttttc attctttatt
    52441 aagcggggtt atgctgcgac aacaatcccc tttttggcaa atgtagctcg taatcggcgg
    52501 gcgaacgcca gagcgtgctc gccgtcgcca tgcaggcaga ccgtttgcgc cgcgaccgtt
    52561 gcccattcgc cggtgatact tttgactctg ccgtgttgca ccatctccag cgtttgcgcc
    52621 aacgcctgtt cttcgttttc aatcaacgcg cctggctgac ttcgcggcac cagcgagccg
    52681 tcagcctgat aaccgcgatc ggcaaacact tcctcgcgcg ttgtcaggcc atattgcttg
    52741 cctgcacgaa tcagctcgct tcctgccagc ccaacgagaa ccaatgccgg atcgcaagcg
    52801 tataccgctc tggcgatggc gtctgccagt tgtgcttctt tcgccgcctg gttgtacaac
    52861 atgccgtgcg gtttgacatg acgcattacg ccgccttgcg cacgggcaat cgttgccagc
    52921 gcgccaattt gatacagcgt ctgggcgtaa acggtttccg gcggcagctg catggcgctg
    52981 cgaccaaaat tttccctgtc gggaaaactc gggtgagcgc caatcgcgac accatttttt
    53041 attgcttcac gcacgcaagc ctgcatggtt tgcgcatcgc ctgcatgaaa tccacaagca
    53101 atattggcag aggaaaccag cgttaatagc tctgcgtcgc tggcgcagcc ttcgcccaga
    53161 tcggcgttca ggtcaatttt cattgtgcag ccgccacgct aattgttcaa aataacgttg
    53221 ctgatcttgc cgcgctttca gtgcctcttc cagtgaacac tggacaaaat gaatcggctg
    53281 accgagcgga atttgcgcca gatggtacat atcagcctca atgatacagg caatacgtgg
    53341 ataaccaccg gtggtctgtg cgtcgttcat caacacaatc ggctgcccgt tatgcggcac
    53401 ctgcaccacg cccggtaaca aaccgtgaga taacagttcg cgatcggtga tgcgttttaa
    53461 aatttgcccc tgtaaacgat agcccattcg gttactttgc gagctaagct gccagggcga
    53521 acgccagaat gcatcctgcg aggcgcgatc gaactcatga tattccggcc ccggcaaggc
    53581 gcgaatgcgg ttgccccaca gcagctgttt aacgccctgc gcttccataa aatcacgctt
    53641 cgatttgcca atcgggagtc agtcaccatc cttcagtaaa cggccttcca gcccgccaat
    53701 ccccactttg agatcggtgc tgcatgaccc cattaccggc ggaacatcaa taccacccgc
    53761 gaccgccaga taactgcgca tcccgtgctg cgggcgttta agcgttaaac gctggcctgc
    53821 tttcatcggc aatcgccagc cggtccagac ggcgttatca tccaaccgcg cttcgcaacc
    53881 tgcacccgtc agggcaaacc acccatcagt ttcgaactca actgttaact gaccgagcgt
    53941 gatctccagc gcgggggcat tggcgtcatt acccaccagc aggttagcaa tgcgcagcgc
    54001 aggcatatcc agtgcgccac agtggctgat acccgactgg caaaaaccgt gacgaccgcc
    54061 atcctgcaca gtggtgtaca tgcccgcacg aataatcttc agcatattcc ctccttctgc
    54121 ggtacaaatc gcacgctgtc tcccggacgt aataagatgg gttcgtcacg cgccggatca
    54181 aacaggctga gtgaggtatg accaatcaac tgccagccac ccggcgttgc cagcggataa
    54241 acaccagtct gcggcccgcc gatcccgaca gaacctgccg gaacgagcaa gcgcggttca
    54301 gcgcgccgtg gcgtgtgtag ttgttccggc aaactcccga gatacgggaa gcccggttga
    54361 aaacctaaaa accagaccac gtattccacg gaggagtgca attcaacaac ctgtttttcg
    54421 ctcaacccgc aatacgccgc gaccaccgcc aaatccggtc cgcctgcacc accgtaaacc
    54481 accggaattt caataaagcg agactcaggt tccagggcct cgctctcctc ccaccagcgt
    54541 tgcaaacgct ctatggcatc cagcgccagc gactcaggat tacgcaaaat caccgtgata
    54601 ttgttcatgc cgggaatggc ttcaaccaca ttcggcatat ccaccagacg ctgcgccagt
    54661 cgccagatcc gtttctggct agccagcgtc accggcggtt ccagttccag cactaccgcc
    54721 gtttcaccta tcagataaca acgcgctcgt tgcactttcg atccctctga ttattcgtta
    54781 tgcaggatta ggaatatcaa taaaggtcac atcaagatcg gtattttcat tcagccactc
    54841 gctcaatgcg cgaataccac cgcgttcagt ggcatggtga cctgcagcat aaaaatgcaa
    54901 tccctgctcg cgggctgaat gaatggtctg ttcagaaact tcgccagtaa taaaagcatc
    54961 cacgccaaaa cgcgcggcgc tatcgataaa actttgcccg ccgcccgtgc accaggcgac
    55021 gcgctggacc acctcaggtc cggtatcgcc acaccataac ggcttacgtc ccagacgcgc
    55081 ttcaatccag gaggctagtt ccattcccgg cacaggcatg gtcagctctc cccacggcac
    55141 caacggctca atttcgccca tgaccgtgat ccccagtaac gccgccagct gtgcgttatt
    55201 gcccagctca ggatgtgcgt caagcggcaa atgccagcca tacaggttga tatcattcgc
    55261 cagcaacgtt tttaaacggt tacgcttcat tccgcgaatg accggagact ctcctttcca
    55321 gaagtagcca tgatgcacaa tgaccgcatc cgcgcccaga cgcactgcct catcgagcaa
    55381 agcctggctg gcggtgacac cggtaacaat tttttgcacc gtctctttgc cttccacctg
    55441 caaaccattc ggcgcgtaat cactaatcgc cgcgctgttc agtttttcgt taatcagttg
    55501 ttccagttcg gtgtttttca tcttcgcgcc ttccgtattc tgatagctac accgttaaca
    55561 tagcgttaca tcgcctgttc gtcgatagcc aatcttttga catattaaga aacaaacccg
    55621 acgccaaaag acgcaaattg tttatatcat cacgcttcag ctaatgtata tttatgcaat
    55681 cagacagcat attgcataaa taatcagatc tgtatcaaag ttttaacttt aaggaaacac
    55741 aagcacgccg cgcaactatt acgtgacgtt gccgtcaatt gtggaattat tatgaataaa
    55801 cacgcatcac aaccgcgcgc tatttactat gtcgttgcgc tgcaaatctg ggaatatttt
    55861 agcttttacg gcatgcgtgc cctgctgatt ctctatctca ccaatcaact aaaatacaac
    55921 gatactcacg cctacgagtt atttagcgcc tactgttcgc tggtgtatgt cacgccaatc
    55981 ctcggtggct ttttggcgga taaagttctc ggcaatcgca tggcggtgat gctgggggcg
    56041 ttgttgatgg cgatcggtca tgtggtgctg ggtgccagtg agatccatcc gtcattcctc
    56101 tatctgtccc tggcgattat cgtctgcggc tatggcctgt ttaaatctaa cgtaagctgt
    56161 ctgctcggcg agctgtatga gccaaccgat ccgcgtcgtg atggcggttt ctcgctgatg
    56221 tatgcggcgg gtaacgtggg gtctattatc gcacctatcg cctgtggtta cgcccaggaa
    56281 gagtacagct gggcgatggg ctttggcctg gcggcggttg gaatgatcgc gggtctggtc
    56341 attttcttat gtggcaatcg tcatttcact catacccgcg gcgttaacaa aaaagtactg
    56401 cgtgcgacaa actttctcct gccgaactgg ggatggctgc tggttctgct ggtggcaacg
    56461 cctgcactga ttaccgtgct gttctggaaa gagtggtcgg tatacgcctt aattgtcgcg
    56521 actattattg gcctgggtgt actggcaaaa atttatcgca aagcagaaaa ccagaaacag
    56581 cggaaggagc tggggctgat tgtgacgctc accttcttca gtatgttgtt ctgggccttc
    56641 gcacaacagg gcggtagctc gattagcctt tatatcgacc gcttcgttaa ccgcgatatg
    56701 tttggttata ccgttccgac cgcgatgttc cagtcgatta atgccttcgc agttatgctg
    56761 tgcggtgtgt tcctggcgtg ggtggttaaa gaaagcgtcg caggtaatcg taccgtgcgc
    56821 atctggggaa aatttgctct tggccttggc ctgatgagcg ccggattctg cattctgacc
    56881 ttaagcgccc gctggtccgc aatgtatggt cactcttctc tgccactgat ggtgttaggc
    56941 ctcgcggtga tgggctttgc ggaactgttt atcgacccgg ttgccatgtc gcaaattacg
    57001 cgcattgaaa ttccgggcgt gaccggcgta ttaaccggca tctacatgct gctttctggc
    57061 gcgatcgcga actatctggc gggcgtgatt gccgatcaga catcgcaggc ttcgtttgat
    57121 gcttccgggg cgatcaacta ctccatcaat gcatatattg aagtatttga tcaaattacc
    57181 tggggcgcac tggcgtgtgt aggactggta ctgatgattt ggctgtatca ggcgctgaaa
    57241 ttcagaaacc gcgcgctggc gctggagtca taataggcca gcaccgatgc ctgatgcgac
    57301 gcttgacgcg tcttatcagg cctacgtatt tcctgcaatt tattgaattt gcacaaattt
    57361 gtaggctgga ttaggcgttc acgccgcatc cggcatctgg gctcgattgc ctgacgcgtc
    57421 tgttatttcc ccttccgcgc cgcctcatac gctgccaacg tttgtactct cgcctctttg
    57481 tgctcgacta tcggttgcgg ataatccagc ttcacacctg ctttctgcgc ccacttccac
    57541 ggctcatgca ccgctttccc tggtacatcg cgcagctccg gtaaccatcg acgaataaac
    57601 tcgccctcac ggtcaaattt ctcgccctga gttatcggat tgaaaatacg aaaatacggc
    57661 gcagcatcag tacccgtaga agccgcccac tgccagccac cgttattagc tgccaaatca
    57721 ccatcaatca gctgcgacat gaaatatcgc tcgccttcgc gccagtcgat caacaaatct
    57781 ttcaccagaa aactggctgt aatcatccgt agccggttat gcatccagcc agtgctgtta
    57841 agctgacgca tagcggcatc aataatcggg tatcccgttt tgcctttctg ccaggcctgt
    57901 aaatgtgcgg gattgctttg ccactgcaca cgatccgtcc aggcaataaa tggacgatgt
    57961 ttacacaacg aggggtaata cgtcatcaga tgacggtaga actcgcgcca gatcagctca
    58021 ctaagccaga cactaccggc cccaccgtcc agcgcctgcg gctgttccgc caacaagcga
    58081 tgcaaacact ggcgaggcga taacccgccc gttgccaagc tggcggacaa acggctggtg
    58141 ccttccactg ccggaaaatc tcgttgttgc tcatactctc cagcaccgtt ctcgcaaaac
    58201 tggcgtaatt gcgcaatcgc cgctttttct tccaccggga aatgcgcagt atcgaaagac
    58261 tgacgaggat aattcagcgt aatggatggc gcgggcttta tcgatccgct actacgaact
    58321 tttggtgcag cgacgcactc cggcatccct tcccgcagcc gtttcagcca ggcattctta
    58381 aaaggcgtaa agactttgta catctcgtga ttgccggtca tcaccgcccc cggcggcagg
    58441 atcacgctgt catcaaatcc ttcacacacc acgttacgca gcgctctttc aacgtccaca
    58501 tcccgcgctc gctcattcac ctcatactga tagttataaa aaagatgagt aacgctgttt
    58561 tccgcgcaca cctgtttaac tatttcgaca ctggcgacaa agtcatccac ttcacggaac
    58621 aataaaggaa tacctttttc cgccagcgcg atttgtagcc cattcagttg agcattgata
    58681 agttcagcct gacgcggcga catgttatgc gtctcccact tgcgtggtgt agcgatatac
    58741 agcgccagca cgcgtgcaga cgaattgcgg caggcggcag ccagtgcgag attatcgtgc
    58801 agacgtaaat cctggcgaaa ccaggccaga tgggtagtca taaaactcct gaatggcgca
    58861 agttaatcac acgcggccaa agttcgaaat aacgctgccc tgaaagccag gcgtcaggat
    58921 aaagcgccat atagtgtttg atacgcatca gcggcgcaag aagtggttgc tcgccacggc
    58981 gataactgtc gataagcgca gccagctgct ggcgttctgt ggaatcaata tgcgggcgaa
    59041 aataaccctg aacgtgcatc aaaacgttgg tgtgatcgcg cgggttggcg ggatgagaaa
    59101 gcaaaacaat tacgcgttgg cggtactggt tgtaaaaatc atccagattt tgccactgat
    59161 gaatatcagc cacaaacggg ccaagtttgc ggtactcggg ctgagaatgg gccagaaaga
    59221 cgagtttata gcggctgtga taatcgagaa gcgccccgcg cgttaagcca tgttctttca
    59281 gtcgatgtaa ctcatgaagg gcgaagatac ggattagggt gctgtcatca aatcgttgta
    59341 gattcattac tgctcctgaa cggtatacag gagtaagtgt aggcgttggt aaatgatgtt
    59401 tgtgaagtta atccgctagc agggcaagat tacgaaagcg cgctccccgc aagcaactaa
    59461 tcaaggtagt taatcatggc tgaacacgat attacgccag aagtcactat ttccaaaaca
    59521 cagcgccgct ataaagtcgg ctatgtcaga gcccgccacc aggaccgcaa aaccggtata
    59581 gcccggtatt attcacaaca tcccagtctg caccttaagg gcgactggct gaaagaggcc
    59641 ggatttgata ccggacgggg tgtcacggtg aagatttcgg aggggggcct gaccatcatc
    59701 gctgacagtg acgaggtgca ggaactgcgg gatgggatgt ttagtgtgct gaacgaaagt
    59761 taaatattaa aaaagctggg aagatttaaa accttcctag ctttttattc tgtcttatta
    59821 taatggatac tcatcatctt ccgatgaatt tataaattgc tccaagttta catgaagagt
    59881 acttaaccta gtttttaact cattaagtga agcgataatt tcatattttc caacataatc
    59941 atatgatgga tcgtattcat tatctatgtc agaagctaaa aatgatgata ttcgtagttt
    60001 aaatttagct tcatctttat tacattcatt ggcataaggt gaatgcccta tcatagcata
    60061 ttcatctaat ctaaaaggat aagatggttt taattcatcg atcaatgaaa tttcaatcca
    60121 tgttctatat ccaggcatta atggactaat ttctttgagc aaaacctttc cacttaataa
    60181 tgtattgaga ttgttcatta ttatgtctcc cacgggaaaa atatatgtct aacacttcca
    60241 ccatgttgat gaacttgtaa atgtggtaaa tcaccatcag gatttcctgg cccatgacct
    60301 gacactctat atgaaggttt tgttacagaa ggatctttta tatcatccca atgatatgaa
    60361 cctgcttttg ttttattttc agtaagatat tccattaagt tcttagtgct ggggcctgac
    60421 tcacctgtca tatttttata attatgacct aaatatctat ccataaacaa ttgttcagcg
    60481 tcacttctag acctaacagt aactttggtt tcactactat ctaataaacg attcgctttt
    60541 tggttactaa cagcacatgg agttaatcca agtggatcag catattttag aggattaggt
    60601 gcataactgt agagatttaa accgcctctc aaccctatcg ggtcagttac catgaaccgc
    60661 ccgacatccg gatcatagta cctgtgcaga ttgtagtgca gccccgtttc cctgtcgagg
    60721 tactgtccct ggtagcgcag gttctgctcc accccgccgt ggtccttctc ctgtatcgtg
    60781 ttaccccaga cctgataccc cgtctcccac acgattttcc cgtcgctgtc cgtcatctct
    60841 tccggcgcgc cgttcacatc cgtgtgaaaa tacagtatcc ggttcgggcc ctctttgccc
    60901 gccttatcca cccgcgccag cggttcataa ctcccctggt ccctgtagat gtacaggctg
    60961 cctgttccct ccggcctgct ctcctgcgcc agccgcattc cgtcccataa aaattttgtc
    61021 cggttgtacg gcttaccgtc agggctgatg tgctgttttt ccgtccgcct ccccagtgca
    61081 tcgtacacat accggtatgt cccccgcgct gtcgtcactt ccaccatccg gtgttccgca
    61141 tcataacggt aactctgcgt tccgctccgg ccctcctttt ccaccgcacg gccaaattcg
    61201 tcataacggt agtgcacacc acggtaagac atcagccggt tgaacggcac cacgctgttt
    61261 tctgccgtgg ccctttcccc cgcgcgcctc cccagcaggt ttcccgccgc gtccacctcc
    61321 gtttccggct ggtgccacac cgggtgccac tgcgcctgct ccacccgccc cagcgggtcg
    61381 tgcgtctccg tgatatgccc ctgcaggtca tacacatagc gccatttgcc gccctgaggg
    61441 tcggtcatcc ccagcaacag tcgttcttcg tcgctccagc ggaatgtcgt cacctgtccg
    61501 gcttcgtcct ggtacgccgt cacctgattc tgcgcatccc actggtgata acgtgtgcgc
    61561 cccagtccgt ccgtcacgca cagcctgcgc tgcgccaggt cccacgccag ccggtactgt
    61621 tcaccgctgt ccgtgtggtg ccccgtcacc cgccatgccg cgtcatccgg accttcatac
    61681 cagccccact ggtaataata cccttcgccg cccggctggc ggtgcagcgt catgcagtgg
    61741 ttttcgtcgt aagcaaactc acgcagcagg tgccccgtgc tgtcgtacac gccgttcagg
    61801 tggccggcct cgtcatagct gtaacgacgc agcagtaaag gtgcagtctc agggaaatgc
    61861 tgataaatct gtgtcacccg ccggggatgc gccgccagtt cgtaatacag acggatgcac
    61921 ggacgctgct gttcgccgct gatttccaca atgcggcccc ggtcgtcgta gtacagattc
    61981 agacagttgc tgttacggtc gcccagcatt ttcagccgct ggcgctgcgg gtggtgcggg
    62041 tcagcctcaa acagaaaaca ctgcccgtca tcgctgctga taagccagtg cccctgctca
    62101 ccacgcctta ccgccagccc ttcaccgggg ctgtaaaacc cgccccccgg cagtacggct
    62161 tccatatcca agcgtcgccc catgcccgac acaaaggtca tgcagttttc gtctgcgcta
    62221 cccggtgtgc acacgaggct gacttcatac attacactcc agccctgacc aaacattcct
    62281 tccgtacgtt cgtcacgact gctgtacacg cgctgccagt ccagcggaaa gtggcccggc
    62341 agactgaaat cgaagtcatc gtcatcggcc aggtatttgg ccccggtggc ggcattgacc
    62401 ggatgcggaa gggcactggt ggccttctgc gtcagcatgc tcatcccgac actggcagca
    62461 aaacaggcga ttttagaaag cattttgccc gggcgaagaa acatcagcgc cacggttgcc
    62521 accagggtta tctgcgacct gccgcttctg atatccctga ccaccagtaa cggaccgcca
    62581 atgcgcacat ccggtgacac aaacgccccg ttccccggct cattcaccac cctggcctcg
    62641 caggtacagc gtgccccgct gcgtgtcgcc ggctggctgt tgatacgcac atcccgcgcg
    62701 ccttccgcga tatattccgc tgcgggcggc tcgtcatcgc ctttgaacag attactgatg
    62761 ctgccaataa ccgcattcac tccctgaaag gcggtttcca gcataccgcc cggattcagc
    62821 aggttaaggg ccgttcccag ggggtcatcc agaaaggcgg cttttttttg tgcgagaaac
    62881 tgcgtcagtg aggaaggatg tttatggcag tcgattttat cgtcaggggt cggggtggat
    62941 cccggggccg cactggccac agtgggctgc catagttccg acacaaaatg tgtcacgcca
    63001 tccaccattt cagaacgccc ttcttccgta ctgagctggt cgtgcagagc ccgggacatc
    63061 agcacaggcc ctgtcaccgg gttaaccgcc agggctatgg cccccgccat ttccagatat
    63121 tccccggcca catcccggaa cgttctggca tcctcctgac gctgcgcttc ttcctgttct
    63181 gccttcgcct gcgcttcctg cgcctctatg tcctgccgcg atgccgcagt cgccgctgcc
    63241 cgcgcggcag gtttgctgtt gatccatgtg tcaccagagc cggtgaggat gtaaccttca
    63301 atctgaggcg ggaaaagcat atttgcgagg gctgaacagg cttcgctgac cttttctgtc
    63361 acgccggtgt acaccatcgc cacacccgcc acaaaaccgg tcagcacgac ggccgtgcac
    63421 cccatcgtga tccccactga cgtcgccagt aacgaggttg tgccgacaat gagggcatca
    63481 ataacgacat ttgcggccac ctccagcacc gcccccagca catctgccat aaacgacgta
    63541 tgcagcaggg catcaccctg tcttgccgcc cagaatgcct cttccatgtc ccgttccctg
    63601 tatgattaat tcgtcctgac ttcctgccgc atcctgaagc gcagattctt tttaatttca
    63661 gcccagcgcg tcatatctgc cggggtcagc ggtgtggttc tggcgtaggt gaaggccatc
    63721 atgacccgct cacccggcaa tacaaatgcc acctgacact ggtacacctg ctcgttaccc
    63781 cggagaaact ggctctgtat ttccaaaccg cctcgttgag ataatattcc gtcataatgt
    63841 aaatagtccg ttttaataaa ggaattattt attgacaggt tctgtttttt taatcatgaa
    63901 tgtaataccc atcaccgctt tacagcacac tttatctttc cagtcataat cactaataat
    63961 attaagcaat tagcaatatt tacaacgacc agagcggata catagttatg tatttaacaa
    64021 tcccaatata ttcactatgc aaataaaacc actagtaaca gacacgctac cgcctccata
    64081 taaaaatgga caccttataa aattgtatct tgcatagaaa aagctgaaat atcggatgcg
    64141 ttttaattat caactataaa tctataagct ttttattaac aaca
//
LOCUS       DAJTRC010000012        60069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.012, whole genome shotgun sequence.
ACCESSION   DAJTRC010000012 DAJTRC010000000
VERSION     DAJTRC010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 60069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 60069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..60069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.012"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            85..879
                     /gene="panB"
                     /locus_tag="OO357_000825"
     CDS             85..879
                     /gene="panB"
                     /locus_tag="OO357_000825"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000805487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HCR5885866.1"
                     /translation="MKPTTIASLQKYKQDKKRFATITAYDYSFAKLFADEGLNVMLVG
                     DSLGMTVQGHDSTLPVTVADIAYHTAAVRRGAPNCLLLADLPFMAYATPEQAFENAAT
                     VMRAGANMVKIEGGEWLVETVKMLTERAVPVCGHLGLTPQSVNIFGGYKVQGRGNEAS
                     DRLLSDALALEAAGAQLLVLECVPVELAKRITEALAIPVIGIGAGNVTDGQILVMHDA
                     FGITGGHIPKFAKNFLAETGDIRAAVRQYMAEVESGVYPGEEHSFH"
     gene            891..1742
                     /gene="panC"
                     /locus_tag="OO357_000826"
     CDS             891..1742
                     /gene="panC"
                     /locus_tag="OO357_000826"
                     /EC_number="6.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HCR5885867.1"
                     /translation="MLIIETLPLLRQQIRRLRMEGKRVALVPTMGNLHNGHMKLVDEA
                     KARADVVVVSIFVNPMQFDRPEDLARYPRTLQEDCEKLNKRKVDLVFAPSVKEIYPNG
                     TETHTYVDVPGLSTMLEGASRPGHFRGVSTIVSKLFNLVQPDIACFGEKDFQQLALIR
                     KMVADMGFDIEIVGVPIMRAKDGLALSSRNGYLTAEQRKIAPGLYKVLSSIADKLQAG
                     ERDLDEIITIAGQELNEKGFRADDIQIRDADTLLEVSETSKRAVILVAAWLGDARLID
                     NKMVELA"
     gene            complement(1816..2718)
                     /gene="rpnC"
                     /locus_tag="OO357_000827"
     CDS             complement(1816..2718)
                     /gene="rpnC"
                     /locus_tag="OO357_000827"
                     /EC_number="3.1.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-promoting nuclease RpnC"
                     /protein_id="HCR5885868.1"
                     /translation="MDAPSTTPHDAVFKQFLMHAETARDFLEIHLPVELRELCDLNTL
                     HLESGSFIEECLKGHSTDVLYSMQMQGNPGYLHVVIEHQSKPDKKMAFRMMRYSIAAM
                     HRHLEAGHDKLPLVVPILFYQGEATPYPLSMCWFDMFYSPELARRVYNSPFPLVDITI
                     TPDDEIMQHRRIAILELLQKHIRQRDLMLLLEQLVTLIDEGYTSGSQLVAMQNYMLQR
                     GHTEQADLFYGVLRDRETGGKSMMTLAQWFEEKGIEKGIQQGRQEERQEFALRLLSKG
                     MSREDVAEMANLPLAEIDKVINLI"
     gene            2992..3372
                     /gene="panD"
                     /locus_tag="OO357_000828"
     CDS             2992..3372
                     /gene="panD"
                     /locus_tag="OO357_000828"
                     /EC_number="4.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate 1-decarboxylase"
                     /protein_id="HCR5885869.1"
                     /translation="MIRTMLQGKLHRVKVTHADLHYEGSCAIDQDFLDAAGILENEAI
                     DIWNVTNGKRFSTYAIAAERGSRIISVNGAAAHCASVGDIVIIASFVTMPDEEARTWR
                     PNVAYFEGDNEMKRTAKAIPVQVA"
     gene            complement(3376..4605)
                     /locus_tag="OO357_000829"
     CDS             complement(3376..4605)
                     /locus_tag="OO357_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5885870.1"
                     /translation="MYKQAVILLLMLFTASVSAALPARYMQTIENAAVWAQISDKMVT
                     VGNIRAGQIIAVEPTAASYYAFNFGFGKGFIDKGHLEPVQGRQKVEDGLGDLNKPLSN
                     QNLITWKDTPVYNAPSVGSAPFGVLADNLRYPILHKLKDRLNQTWYQIRIGDRLAYIS
                     ALDAQPDNGLPVLTYHHILRDEENTRFRHTSTTTSVRAFNNQMAWLRDRGYATLSMAQ
                     LEGYVKNTINLPARAVVITFDDGLKSVSRYAYPVLKQYGMKATVFIVTSRIKRHPQKW
                     NPKSLQFMSVSELNEIRDVFDFQSHTHFLHRVDGYRRPILLSRSEHNILFDFARSRRA
                     LAQFNPHVWYLSYPFGGFNDKAVKAANDAGFHLAVTTMKGKVKPGDNPLLLKRLYILR
                     TDSLEKMSRLVSNQPQG"
     gene            complement(4669..5109)
                     /locus_tag="OO357_000830"
     CDS             complement(4669..5109)
                     /locus_tag="OO357_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5885871.1"
                     /translation="MLGWVITCHDDRAQEILDALEKKHGALLQCRAVNFWRGLSSNML
                     SRMMCDALHEADSGEGVIFLTDIAGAPPYRVASLLSHKHSRCEVISGVTLPLIEQMMA
                     CRETMTSSEFRECIVELGGPEVSSLWHQQQKNPPFVLKHNLYEY"
     gene            complement(5214..5984)
                     /locus_tag="OO357_000831"
     CDS             complement(5214..5984)
                     /locus_tag="OO357_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001747261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5885872.1"
                     /translation="MMHLYWVALKSIWAKEIHRFMRIWVQTLVPPVITMTLYFIIFGN
                     LIGSRIGDMHGFSYMQFIVPGLIMMSVITNAYANVASSFFGAKFQRNIEELLVAPVPT
                     HVIIAGYVGGGVARGLFVGILVTAISLFFVPFQVHSWVFVALTLVLTAVLFSLAGLLN
                     GVFAKTFDDISLVPTFVLTPLTYLGGVFYSLTLLPPFWQGLSHLNPIVYMISGFRYGF
                     LGINDVPLVTTFGVLVVFIVAFYLICWSLIQRGRGLRS"
     gene            complement(5981..6907)
                     /locus_tag="OO357_000832"
     CDS             complement(5981..6907)
                     /locus_tag="OO357_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5885873.1"
                     /translation="MTIALELQQLKKNYPGGVQALRGIDLQVEAGDFYALLGPNGAGK
                     STTIGIISSLVNKTSGRVSVFGYDLEKDVVNAKRQLGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVERKEAYIRSEKYLKQLDLWGKRNERARMLSGGMKRRLMIARALMHEPKLLIL
                     DEPTAGVDIELRRSMWGFLKDLNDKGTTIILTTHYLEEAEMLCRNIGIIQHGELVENT
                     SMKALLAKLKSETFILDLAPKSPLPKLDGYQYRLVDTATLEVEVLREQGINSVFTQLS
                     EQGIQVLSMRNKANRLEELFVSLVNEKQGDRA"
     gene            7016..7678
                     /gene="can"
                     /locus_tag="OO357_000833"
     CDS             7016..7678
                     /gene="can"
                     /locus_tag="OO357_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonate dehydratase"
                     /protein_id="HCR5885874.1"
                     /translation="MKDIDTLISNNALWSKMLVEEDPGFFEKLAQAQKPRFLWIGCSD
                     SRVPAERLTGLEPGELFVHRNVANLVIHTDLNCLSVVQYAVDVLEVEHIIICGHYGCG
                     GVQAAVENPELGLINNWLLHIRDIWFKHSSLLGEMPQERRLDTLCELNVMEQVYNLGH
                     STIMQSAWKRGQKVTIHGWAYGIHDGLLRDLDVTATNRETLEQRYRHGISNLKLKHAN
                     HK"
     gene            complement(7719..8255)
                     /gene="hpt"
                     /locus_tag="OO357_000834"
     CDS             complement(7719..8255)
                     /gene="hpt"
                     /locus_tag="OO357_000834"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004204384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HCR5885875.1"
                     /translation="MKHTVEVMIPEAEIKARIAELGRQITERYKDSGSDMVLVGLLRG
                     SFMFMADLCREVQVSHEVDFMTASSYGSGMSTTRDVKILKDLDEDIRGKDVLIVEDII
                     DSGNTLSKVREILSLREPKSLAICTLLDKPSRREVNVPVEFIGFSIPDEFVVGYGIDY
                     AQRYRHLPYIGKVILLDE"
     gene            8461..10851
                     /gene="gcd"
                     /locus_tag="OO357_000835"
                     /pseudo
     CDS             8461..10851
                     /gene="gcd"
                     /locus_tag="OO357_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414666.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="quinoprotein glucose dehydrogenase"
     gene            complement(10898..12448)
                     /gene="cueO"
                     /locus_tag="OO357_000836"
     CDS             complement(10898..12448)
                     /gene="cueO"
                     /locus_tag="OO357_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase CueO"
                     /protein_id="HCR5885876.1"
                     /translation="MQRRDFLKYSVALGVASALPLWSRAVFAAERPTLPIPDLLTTDA
                     RNRIQLTIGAGQSTFGEKTATTWGYNGNLLGPAVKLQRGKAVTVDIYNQLAEETTLHW
                     HGLEVPGDVDGGPQGIIPPGGKRSVTLNVDQPAATCWFHPHQHGKTGRQVAMGLAGLV
                     VIEDDEILKLMLPKQWGIDDVPVIVQDKKFSADGQIDYQLDVMTAAVGWFGDTLLTNG
                     AIYPQHAAPRGWLRLRLLNGCNARSLNFATSDNRPLYVIASDGGLLPEPVKVSELPVL
                     MGERFEVLVEVNDNKPFDLVTLPVSQMGMAIAPFDKPHPVMRIQPIAISASGALPDTL
                     SSLPALPSLEGLTVRKLQLSMDPMLDMMGMQMLMEKYGDQAMAGMDHSQMMGHMGHGN
                     MNHMNHGGKFDFHHANKINGQAFDMNKPMFAAAKGQYERWVISGVGDMMLHPFHIHGT
                     QFRILSENGKPPAAHRAGWKDTVKVEGNVSEVLVKFNHNAPKEHAYMAHCHLLEHEDT
                     GMMLGFTV"
     gene            12614..12961
                     /locus_tag="OO357_000837"
     CDS             12614..12961
                     /locus_tag="OO357_000837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacC family pilotin-like protein"
                     /protein_id="HCR5885877.1"
                     /translation="MKTFFRTVLFGSLIAVCANSYALSESEAEDMADLTAVFVFLKND
                     CGYQNLSNGQIRRALVFFAQQNQWDLSNYDTFDMKALGEDSYRDLSGIGIPVAKKCKA
                     LARDSLSLLAYVK"
     gene            13067..13933
                     /gene="speE"
                     /locus_tag="OO357_000838"
     CDS             13067..13933
                     /gene="speE"
                     /locus_tag="OO357_000838"
                     /EC_number="2.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyamine aminopropyltransferase"
                     /protein_id="HCR5885878.1"
                     /translation="MAEKKQWHETLHDQFGQYFAVDNVLYHEKTDHQDLIIFENAAFG
                     RVMALDGVVQTTERDEFIYHEMMTHVPLLAHGHAKHVLIIGGGDGAMLREVTRHKNVE
                     SITMVEIDAGVVSFCRQYLPNHNAGSYDDPRFQLVIDDGVNFVNQTSQTFDVIISDCT
                     DPIGPGESLFTSAFYEGCKRCLNPGGIFVAQNGVCFLQQEEAIDSHRKLSHYFSDVGF
                     YQAAIPTYYGGIMTFAWATDNDALRHLSTEIIQARFLASGLKCRYYNPAIHTAAFALP
                     QYLQDALASQPS"
     gene            13949..14743
                     /gene="speD"
                     /locus_tag="OO357_000839"
     CDS             13949..14743
                     /gene="speD"
                     /locus_tag="OO357_000839"
                     /EC_number="4.1.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine decarboxylase"
                     /protein_id="HCR5885879.1"
                     /translation="MKKLKLHGFNNLTKSLSFCIYDICYAKTAEERDGYIAYIDELYN
                     ANRLTEILSETCSIIGANILNIARQDYEPQGASVTILVSEEPVDPKLIDKTEHPGPLP
                     ETVVAHLDKSHICVHTYPESHPEGGLCTFRADIEVSTCGVISPLKALNYLIHQLESDI
                     VTIDYRVRGFTRDINGMKHFIDHEINSIQNFMSDDMKALYDMVDVNVYQENIFHTKML
                     LKEFDLKHYMFHTKPEDLTDSERQEITAALWKEMREIYYGRNMPAV"
     gene            complement(14781..15143)
                     /locus_tag="OO357_000840"
     CDS             complement(14781..15143)
                     /locus_tag="OO357_000840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YacL family protein"
                     /protein_id="HCR5885880.1"
                     /translation="MDYEFLRDITGVVKVRMSMGHEVVGHWFNEEVKENLALLDEVEQ
                     AAHALKGSERSWQRAGHEYTLWMDAEEVMVRANQLEFAGDEMEEGMNYYDEESLSLCG
                     VEDFLQVVAAYRNFVQQK"
     gene            complement(15318..17915)
                     /gene="acnB"
                     /locus_tag="OO357_000841"
     CDS             complement(15318..17915)
                     /gene="acnB"
                     /locus_tag="OO357_000841"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019080489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aconitate hydratase
                     2/2-methylisocitrate dehydratase"
                     /protein_id="HCR5885881.1"
                     /translation="MLEEYRKHVAERAAEGIAPKPLDANQMAALVELLKNPPAGEEEF
                     LLDLLTNRVPPGVDEAAYVKAGFLAAIAKGEAKSPLLTPEKAIELLGTMQGGYNIHPL
                     IDALDDAKLAPIAAKALSHTLLMFDNFYDVEEKAKAGNEYAKQVMQSWADAEWFLNRP
                     ALAEKLTVTVFKVTGETNTDDLSPAPDAWSRPDIPLHALAMLKNAREGIEPDQPGVVG
                     PIKQIEALQQKGFPLAYVGDVVGTGSSRKSATNSVLWFMGDDIPHVPNKRGGGLCLGG
                     KIAPIFFNTMEDAGALPIEVSVSNLNMGDVIDVYPYKGEVRNHETGELLATFELKTDV
                     LIDEVRAGGRIPLIIGRGLTTKAREALGLPHSDVFRQAKDVAESDRGFSLAQKMVGRA
                     CGVKGIRPGAYCEPKMTSVGSQDTTGPMTRDELKDLACLGFSADLVMQSFCHTAAYPK
                     PVDVNTHHTLPDFIMNRGGVSLRPGDGVIHSWLNRMLLPDTVGTGGDSHTRFPIGISF
                     PAGSGLVAFAAATGVMPLDMPESVLVRFKGKMQPGITLRDLVHAIPLYAIKQGLLTVE
                     KKGKKNIFSGRILEIEGLPDLKVEQAFELTDASAERSAAGCTIKLNKEPIIEYLSSNI
                     VLLKWMIAEGYGDRRTLERRIQGMEKWLANPELLEADADAEYAAVIDIDLAEIKEPIL
                     CAPNDPDDARPLSAVQGEKIDEVFIGSCMTNIGHFRAAGKLLDAHKGQLPTRLWVAPP
                     TRMDAAQLTEEGYYSVFGKSGARIEIPGCSLCMGNQARVADGATVVSTSTRNFPNRLG
                     TGANVFLASAELAAVAALIGKLPTPEEYQTYVAQVDKTAVDTYRYLNFNQLSQYTEKA
                     DGVIFQTAV"
     gene            18270..20123
                     /locus_tag="OO357_000842"
                     /pseudo
     CDS             18270..20123
                     /locus_tag="OO357_000842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3300 domain-containing protein"
     gene            complement(20194..21618)
                     /gene="lpdA"
                     /locus_tag="OO357_000843"
     CDS             complement(20194..21618)
                     /gene="lpdA"
                     /locus_tag="OO357_000843"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HCR5885882.1"
                     /translation="MSTEIKTQVVVLGAGPAGYSAAFRCADLGLETVIVERYNTLGGV
                     CLNVGCIPSKALLHVAKVIEEAKALAEHGIVFGEPKTDIDKIRTWKEKVINQLTGGLA
                     GMAKGRKVKVVNGLGKFTGANTLEVEGENGKTVINFDNAIIAAGSRPIQLPFIPHEDP
                     RIWDSTDALELKEVPERLLVMGGGIIGLEMGTVYHALGSQIDVVEMFDQVIPAADKDI
                     VKVFTKRISKKFNLMLETKVTAVEAKEDGIYVTMEGKKAPAEPQRYDAVLVAIGRVPN
                     GKNLDAGKAGVEVDDRGFIRVDKQLRTNVPHIFAIGDIVGQPMLAHKGVHEGHVAAEV
                     IAGKKHYFDPKVIPSIAYTEPEVAWVGLTEKEAKEKGISYETATFPWAASGRAIASDC
                     ADGMTKLIFDKESHRVIGGAIVGTNGGELLGEIGLAIEMGCDAEDIALTIHAHPTLHE
                     SVGLAAEVFEGSITDLPNPKAKKK"
     gene            complement(21943..23823)
                     /gene="aceF"
                     /locus_tag="OO357_000844"
     CDS             complement(21943..23823)
                     /gene="aceF"
                     /locus_tag="OO357_000844"
                     /EC_number="2.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex
                     dihydrolipoyllysine-residue acetyltransferase"
                     /protein_id="HCR5885883.1"
                     /translation="MAIEIKVPDIGADEVEITEILVKVGDKVEAEQSLITVEGDKASM
                     EVPSPQAGIVKEIKVSVGDKTQTGALIMIFDSADGAADAAPAQAEEKKEAAPAAAPAA
                     AAAKDVNVPDIGSDEVEVTEILVKVGDKVEAEQSLITVEGDKASMEVPAPFAGTVKEI
                     KVNVGDKVSTGSLIMVFEVAGEAGAAAPAAKQEATPAATPAPAAGVKEVNVPDIGGDE
                     VEVTEVMVKVGDKVAAEQSLITVEGDKASMEVPAPFAGVVKELKVNVGDKVKTGSLIM
                     IFEVEGAAPAAAPAKQEAAAPAPAAKAEAPAAKAEGKSEFAENDAYVHATPLIRRLAR
                     EFGVNLAKVKGTGRKGRILREDVQAYVKEAIKRAEAAPAATGGGIPGMLPWPKVDFSK
                     FGEIEEVELGRIQKISGANLSRNWVMIPHVTHFDKTDITELEAFRKQQNEEAAKRKLD
                     VKITPVVFIMKAVAAALEQMPRFNSSLSEDGQRLTLKKYINIGVAVDTPNGLVVPVFK
                     DVNKKGIIELSRELMTISKKARDGKLTAGEMQGGCFTISSIGGLGTTHFAPIVNAPEV
                     AILGVSKSAMEPVWNGKEFVPRLMLPISLSFDHRVIDGADGARFITIINNTLSDIRRL
                     VM"
     gene            complement(23838..26501)
                     /gene="aceE"
                     /locus_tag="OO357_000845"
     CDS             complement(23838..26501)
                     /gene="aceE"
                     /locus_tag="OO357_000845"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008814576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HCR5885884.1"
                     /translation="MSERFPNDVDPIETRDWLQAIESVIREEGVERAQYLIDQLLAEA
                     RKGGVNVAAGTGISNYINTIPVEEQPEYPGNLELERRIRSAIRWNAIMTVLRASKKDL
                     ELGGHMASFQSSATIYDVCFNHFFRARNEQDGGDLVYFQGHISPGVYARAFLEGRLTQ
                     EQLDNFRQEVHGNGLSSYPHPKLMPEFWQFPTVSMGLGPIGAIYQAKFLKYLEHRGLK
                     DTSKQTVYAFLGDGEMDEPESKGAITIATREKLDNLVFVINCNLQRLDGPVTGNGKII
                     NELEGIFEGAGWNVIKVMWGSRWDELLRKDTSGKLIQLMNETVDGDYQTFKSKDGAYV
                     REHFFGKYPETAALVADWTDEQIWALNRGGHDPKKIYAAFKKAQETKGKATVILAHTI
                     KGYGMGDAAEGKNIAHQVKKMNMDGVRHIRDRFNVPVSDADIEKLPYITFPEGSEEHT
                     YLHAQRQKLHGYLPSRQPNFTEKLELPSLQDFGALLEEQSKELSTTIAFVRALNVMLK
                     NKSIKDRLVPIIADEARTFGMEGLFRQIGIYSPNGQQYTPQDREQVAYYKEDEKGQIL
                     QEGINELGAGCSWLAAATSYSTNNLPMIPFYIYYSMFGFQRIGDLCWAAGDQQARGFL
                     IGGTSGRTTLNGEGLQHEDGHSHIQSLTIPNCISYDPAYAYEVAVIMHDGLERMYGEK
                     QENVYYYITTLNENYHMPAMPEGAEEGIRKGIYKLETIEGSKGKVQLLGSGSILRHVR
                     EAAEILAKDYGVGSDVYSVTSFTELARDGQDCERWNMLHPLETPRVPYIAQVMNDAPA
                     VASTDYMKLFAEQVRTYVPADDYRVLGTDGFGRSDSRENLRHHFEVDASYVVVAALGE
                     LAKRGEIDKKVVADAIAKFNIDADKVNPRLA"
     gene            complement(26662..27426)
                     /gene="pdhR"
                     /locus_tag="OO357_000846"
     CDS             complement(26662..27426)
                     /gene="pdhR"
                     /locus_tag="OO357_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706067.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase complex transcriptional
                     repressor PdhR"
                     /protein_id="HCR5885885.1"
                     /translation="MAYSKIRQPKLSDVIEQQLEFLILEGTLRPGEKLPPERELAKQF
                     DVSRPSLREAIQRLEAKGLLLRRQGGGTFVQSSLWQSFSDPLVELLSDHPESQYDLLE
                     TRHALEGIAAYYAALRSTDEDKERIRELHHAIELAQQSGDLDAESNAVLQYQIAVTEA
                     AHNVVLLHLLRCMEPMLAQNVRQNFELLYSRREMLPLVSSHRTRIFEAIMVGKPEEAR
                     EASHRHLAFIEEILLDRSREESRRERSLRRLEQRKN"
     gene            27967..29340
                     /gene="aroP"
                     /locus_tag="OO357_000847"
     CDS             27967..29340
                     /gene="aroP"
                     /locus_tag="OO357_000847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transporter AroP"
                     /protein_id="HCR5885886.1"
                     /translation="MMEGQQHGEQLKRGLKNRHIQLIALGGAIGTGLFLGSASVIQSA
                     GPGIILGYAIAGFIAFLIMRQLGEMVVEEPVAGSFSHFAYKYWGSFAGFASGWNYWVL
                     YVLVAMAELTAVGKYIQFWYPEIPTWVSAAVFFVVINAINLTNVKVFGEMEFWFAIIK
                     VIAVVAMIIFGGWLLFSGNGGPQASVSNLWDQGGFLPHGFTGLVMMMAIIMFSFGGLE
                     LVGITAAEADNPEQSIPKATNQVIYRILIFYIGSLAVLLSLMPWTRVTADTSPFVLIF
                     HELGDTFVANALNIVVLTAALSVYNSCVYCNSRMLFGLAQQGNAPKALASVDKRGVPV
                     NTILVSALVTALCVLINYLAPESAFGLLMALVVSALVINWAMISLAHMKFRRAKQEQG
                     VVTRFPALLYPLGNWICLLFMAAVLVIMLMTPGMAISVYLIPVWLIVLGIGYLFKEKT
                     AKAVKAH"
     gene            complement(29383..30237)
                     /gene="ampE"
                     /locus_tag="OO357_000848"
     CDS             complement(29383..30237)
                     /gene="ampE"
                     /locus_tag="OO357_000848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase regulator AmpE"
                     /protein_id="HCR5885887.1"
                     /translation="MTLFTTLLVLIFERLFKLGEHWQLDHRLEAFFRRVKHFSLGRTL
                     GMTIIAMGVTFLLLRALQGVLFNVPTLLVWLLIGLLCIGAGKVRLHYHAYLTAASRND
                     SHARATMAGELTMIHGVPAGCDEREYLRELQNALLWINFRFYLAPLFWLIVGGTWGPV
                     TLMGYAFLRAWQYWLARYQTPHHRLQSGIDAVLHVLDWVPVRLAGVVYALIGHGEKAL
                     PAWFASLGDFHTSQYQVLTRLAQFSLAREPHVDKVETPKAAVSMAKKTSFVVVVVIAL
                     LTIYGALV"
     gene            complement(30234..30785)
                     /gene="ampD"
                     /locus_tag="OO357_000849"
     CDS             complement(30234..30785)
                     /gene="ampD"
                     /locus_tag="OO357_000849"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,6-anhydro-N-acetylmuramyl-L-alanine amidase
                     AmpD"
                     /protein_id="HCR5885888.1"
                     /translation="MLLEQGWLVGARRVPSPHYDCRLDDETPTLLVVHNISLPPGEFG
                     GPWIDALFTGTIDPQAHPFFAEIAHLRVSAHCLIRRDGEIVQYVPFDKRAWHAGVSQY
                     QGRERCNDFSIGIELEGTDTLAYTDAQYQQLAAVTRALIDRYPDIANNMTGHCDIAPD
                     RKTDPGPAFDWARFRALVSKETT"
     gene            30873..31766
                     /gene="nadC"
                     /locus_tag="OO357_000850"
     CDS             30873..31766
                     /gene="nadC"
                     /locus_tag="OO357_000850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HCR5885889.1"
                     /translation="MPPRRYNPDTRRDELLERINLDIPGAVAQALREDLGGTVDANND
                     ITAKLLPENSRSHATVITRENGVFCGKRWVEEVFIQLAGDDVTIIWHVDDGDVINANQ
                     PLFELEGPSRVLLTGERTALNFVQTLSGVASKVRHYVELLEGTNTQLLDTRKTLPGLR
                     SALKYAVLCGGGANHRLGLSDAFLIKENHIIVSGSVRQAVEKASWLHPDAPVEVEVEN
                     LEELDEALKAGADIIMLDNFETEQMREAVKRTNGKALLEVSGNVTDKTLREFAETGVD
                     FISVGALTKHVQALDLSMRFR"
     gene            31969..32409
                     /gene="ppdD"
                     /locus_tag="OO357_000851"
     CDS             31969..32409
                     /gene="ppdD"
                     /locus_tag="OO357_000851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent pilin"
                     /protein_id="HCR5885890.1"
                     /translation="MDKQRGFTLIELMVVIGIIAILSSIGIPAYQNYLRKAALTDMLQ
                     TFVPYRTAVELCALEHGGLDTCDGGSNGIPSPTTTRYVSAMSVAKGVVSLTGQESLNG
                     LSVVMTPGWDNANGVTGWARNCNIQSDSALQQACEDVFRFDDAN"
     gene            32419..33566
                     /gene="tadA"
                     /locus_tag="OO357_000852"
                     /pseudo
     CDS             32419..33566
                     /gene="tadA"
                     /locus_tag="OO357_000852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
     gene            33556..34758
                     /gene="hofC"
                     /locus_tag="OO357_000853"
     CDS             33556..34758
                     /gene="hofC"
                     /locus_tag="OO357_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein transport protein HofC"
                     /protein_id="HCR5885891.1"
                     /translation="MASKQLWRWHGITGDGNAQDGMLWAESRTLLLMALQQQMVTPLS
                     LKRIAINSAQWRGDKSAEVIHQLATLLKAGLTLSEGLALLAEQHPSKQWQALLQSLAH
                     DLEQGIAFSNALLPWSEAFPPLYQAMIRTGELTGKLDECCFELARQQKSQRQLTDKVK
                     SALRYPIIILAMAIMVVVAMLHFVLPEFAAIYKTFNTPLPALTQGIMTLADFSGEWGW
                     LLVLFGFLLAIANKLLMRRPTWLIARQKLLLRIPIMGSLMRGQKLTQIFTILALTQSA
                     GITFLQGVESVRETMRCPYWVQLLTQIQHDISNGHPIWLALKNAGEFSPLCLQLVRTG
                     EASGSLDLMLDNLAHHHRDNTMALADNLAALLEPALLIITGGIIGTLVVAMYLPIFHL
                     GDAMSGMG"
     gene            complement(34793..35836)
                     /gene="guaC"
                     /locus_tag="OO357_000854"
     CDS             complement(34793..35836)
                     /gene="guaC"
                     /locus_tag="OO357_000854"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008461816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HCR5885892.1"
                     /translation="MRIEEDLKLGFKDVLIRPKRSTLKSRSDVELERQFTFKHSGQSW
                     SGVPIIAANMDTVGTFSMASALASFDILTAVHKHFSVEEWQAFINNSSADVLKHVMVS
                     TGTSDADFEKTKQILDLNPALNFVCIDVANGYSEHFVQFVAKAREAWPTKTICAGNVV
                     TGEMCEELILSGADIVKVGIGPGSVCTTRVKTGVGYPQLSAVIECADAAHGLGGMIVS
                     DGGCTTPGDVAKAFGGGADFVMLGGMLAGHEESGGRIVEENGEKFMLFYGMSSESAMK
                     RHVGGVAEYRAAEGKTVKLPLRGQVENTARDILGGLRSACTYVGASRLKELTKRTTFI
                     RVLEQENRIFNNL"
     gene            complement(35998..36036)
                     /locus_tag="OO357_000855"
     CDS             complement(35998..36036)
                     /locus_tag="OO357_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885893.1"
                     /translation="MIIKTLKMSARK"
     gene            36061..36681
                     /gene="coaE"
                     /locus_tag="OO357_000856"
     CDS             36061..36681
                     /gene="coaE"
                     /locus_tag="OO357_000856"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HCR5885894.1"
                     /translation="MGYIVALTGGIGSGKSTVANAFADLGINVIDADIIARQVVEPGA
                     PALHAIADHFGENMIAADGTLQRRALRERIFANPEEKNWLNDLLHPLIQQETQHQIQQ
                     ATSPYVLWVVPLLVENSLYKKANRVLVVDVSPETQLKRTMQRDDVTREHVEQILAAQA
                     TREARLAVADDVIDNNGAPDAIASDVARLHAHYLQLASQFVSQEKP"
     gene            36681..37424
                     /gene="zapD"
                     /locus_tag="OO357_000857"
     CDS             36681..37424
                     /gene="zapD"
                     /locus_tag="OO357_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapD"
                     /protein_id="HCR5885895.1"
                     /translation="MQTQVLFEHPLNEKMRTWLRIEFLIQQLTVNLPIVDHAGALHFF
                     RNVSELLDVFERGEVRTELLKELNRQQRKLQTWIGVPGVDQSRIEALIQQLKAAVSVL
                     ISAPRIGQFLREDRLIALVRQRLSIPGGCCSFDLPTLHIWLHLPQAQRDSQVETWIAS
                     LNPLTQALTMVLDLIRQSAPFRKQTSLNGFYQDNGGDADLLRLNLSLDSQLYPQISGH
                     KSRFAIRFMPLDTENGQVPERLDFELACC"
     gene            37434..37631
                     /gene="yacG"
                     /locus_tag="OO357_000858"
     CDS             37434..37631
                     /gene="yacG"
                     /locus_tag="OO357_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HCR5885896.1"
                     /translation="MSETITVNCPTCGKTVVWGEISPFRPFCSKRCQLIDLGEWAAEE
                     KRIPSSGDLSESDDWSEEPKQ"
     gene            complement(37731..38120)
                     /gene="mutT"
                     /locus_tag="OO357_000859"
     CDS             complement(37731..38120)
                     /gene="mutT"
                     /locus_tag="OO357_000859"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxo-dGTP diphosphatase MutT"
                     /protein_id="HCR5885897.1"
                     /translation="MKKLQIAVGIIRNENNEIFITRRAADAHMANKLEFPGGKIEMGE
                     TPEQAVVRELQEEVGITPQHFSLFEKLEYEFPDRHITLWFWLVESWEGGPWGKEGQPG
                     EWMSLVGLNADDFPPANEPVIAKLKRL"
     gene            complement(38180..40885)
                     /gene="secA"
                     /locus_tag="OO357_000860"
     CDS             complement(38180..40885)
                     /gene="secA"
                     /locus_tag="OO357_000860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HCR5885898.1"
                     /translation="MLIKLLTKVFGSRNDRTLRRMRKVVNIINAMEPEMEKLSDEELK
                     GKTAEFRARLEKGEVLENLIPEAFAVVREASKRVFGMRHFDVQLLGGMVLNERCIAEM
                     RTGEGKTLTATLPAYLNALTGKGVHVVTVNDYLAQRDAENNRPLFEFLGLTVGINLPG
                     MPAPAKREAYAADITYGTNNEYGFDYLRDNMAFSPEERVQRKLHYALVDEVDSILIDE
                     ARTPLIISGPAEDSSEMYKRVNKIIPHLIRQEKEDSETFQGEGHFSVDEKSRQVNLTE
                     RGLVLIEELLVKEGIMDEGESLYSPANIMLMHHVTAALRAHALFTRDVDYIVKDGEVI
                     IVDEHTGRTMQGRRWSDGLHQAVEAKEGVQIQNENQTLASITFQNYFRLYEKLAGMTG
                     TADTEAFEFSSIYKLDTVVVPTNRPMIRKDLPDLVYMTEAEKIQAIIEDIKERTAKGQ
                     PVLVGTISIEKSELVSNELTKAGIKHNVLNAKFHANEAAIVAQAGYPAAVTIATNMAG
                     RGTDIVLGGSWQAEVAALENPTAEQIEKIKADWQVRHDAVLEAGGLHIIGTERHESRR
                     IDNQLRGRSGRQGDAGSSRFYLSMEDALMRIFASDRVSGMMRKLGMKPGEAIEHPWVT
                     KAIANAQRKVESRNFDIRKQLLEYDDVANDQRRAIYSQRNELLDVSDVSETINSIRED
                     VFKATIDAYIPPQSLEEMWDIPGLQERLKNDFDLDLPITEWLDKEPELHEETLRERIL
                     AQSIEVYQRKEEVVGAEMMRHFEKGVMLQTLDSLWKEHLAAMDYLRQGIHLRGYAQKD
                     PKQEYKRESFSMFAAMLESLKYEVISTLSKVQVRMPEEVEELEQQRRMEAERLAQMQQ
                     LSHQDDDSAAAAALAAQTGERKVGRNDPCPCGSGKKYKQCHGRLQ"
     gene            complement(40947..41459)
                     /gene="secM"
                     /locus_tag="OO357_000861"
     CDS             complement(40947..41459)
                     /gene="secM"
                     /locus_tag="OO357_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414639.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secA regulator SecM"
                     /protein_id="HCR5885899.1"
                     /translation="MSGILTRWRQFGKRYFWPHLLLGMVAASLGLPALSNAAEPNAPA
                     KATTRNHEPSAKVNFGQLALLEANTRRPNSNYSVDYWHQHAIRTVIRHLSFAMAPQTL
                     PVAEESLPLQAQHLALLDTLSALLTQEGTPSEKGYRIDYAHFTPQAKFSTPVWISQAQ
                     GIRAGPQRLT"
     gene            complement(41690..42607)
                     /gene="lpxC"
                     /locus_tag="OO357_000862"
     CDS             complement(41690..42607)
                     /gene="lpxC"
                     /locus_tag="OO357_000862"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HCR5885900.1"
                     /translation="MIKQRTLKRIVQATGVGLHTGKKVTLTLRPAPANTGVIYRRTDL
                     NPPVDFPADAKSVRDTMLCTCLVNEHDVRISTVEHLNAALAGLGIDNIVIEVNAPEIP
                     IMDGSAAPFVYLLLDAGIDELNCAKKFVRIKETVRVEDGDKWAEFKPYNGFSLDFTID
                     FNHPAIDSSNQRYAMNFSADAFMRQISRARTFGFMRDIEYLQSRGLCLGGSFDCAIVV
                     DDYRVLNEDGLRFEDEFVRHKMLDAIGDLFMCGHNIIGAFTAYKSGHALNNKLLQAVL
                     AKQEAWEYVTFQDDAELPLAFKAPSAVLA"
     gene            complement(42708..43859)
                     /gene="ftsZ"
                     /locus_tag="OO357_000863"
     CDS             complement(42708..43859)
                     /gene="ftsZ"
                     /locus_tag="OO357_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015965916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HCR5885901.1"
                     /translation="MFEPMELTNDAVIKVIGVGGGGGNAVEHMVRERIEGVEFFAVNT
                     DAQALRKTAVGQTIQIGSGITKGLGAGANPEVGRNAADEDRDALRAALEGADMVFIAA
                     GMGGGTGTGAAPVVAEVAKDLGILTVAVVTKPFNFEGKKRMAFAEQGITELSKHVDSL
                     ITIPNDKLLKVLGRGISLLDAFGAANDVLKGAVQGIAELITRPGLMNVDFADVRTVMS
                     EMGYAMMGSGVASGEDRAEEAAEMAISSPLLEDIDLSGARGVLVNITAGFDLRLDEFE
                     TVGNTIRAFASDNATVVIGTSLDPDMNDELRVTVVATGIGMDKRPEITLVTNKQVQQP
                     VMDRYQQHGMAPLTQEQKPVAKVVNDNAPQTAKEPDYLDIPAFLRKQAD"
     gene            complement(43920..45182)
                     /gene="ftsA"
                     /locus_tag="OO357_000864"
     CDS             complement(43920..45182)
                     /gene="ftsA"
                     /locus_tag="OO357_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HCR5885902.1"
                     /translation="MIKATDRKLVVGLEIGTAKVAALVGEVLPDGMVNIIGVGSCPSR
                     GMDKGGVNDLESVVKCVQRAIDQAELMADCQISSVYLALSGKHISCQNEIGMVPISEE
                     EVTQEDVENVVHTAKSVRVRDEHRVLHVIPQEYAIDYQEGIKNPVGLSGVRMQAKVHL
                     ITCHNDMAKNIVKAVERCGLKVDQLIFAGLASSYSVLTEDERELGVCVVDIGGGTMDI
                     AVYTGGALRHTKVIPYAGNVVTSDIAYAFGTPPSDAEAIKVRHGCALGSIVGKDESVE
                     VPSVGGRPPRSLQRQTLAEVIEPRYTELLNLVNEEILQLQEKLRQQGVKHHLAAGIVL
                     TGGAAQIEGLAACAQRVFHTQVRIGAPLNITGLTDYAQEPYYSTAVGLLHYGKESHLN
                     GEAEVEKRVTASVGSWIKRLNSWLRKEF"
     gene            complement(45179..46009)
                     /gene="ftsQ"
                     /locus_tag="OO357_000865"
     CDS             complement(45179..46009)
                     /gene="ftsQ"
                     /locus_tag="OO357_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ"
                     /protein_id="HCR5885903.1"
                     /translation="MSQAALNTRNSEEEVSSRRNNGTRLAGILFLLTVLTTVLVSGWV
                     VLSWMEDAQRLPLSKLVLTGERHYTRNDDIRQSILALGEPGTFMTQDVNIIQTQIEQR
                     LPWIKQVSVRKQWPDELKIHLVEYVPIARWNDQHMVDAEGNTFSVPPDRTSKQVLPML
                     YGPEGSANEVLQGYREMGQMLAKDRFTLKEAAMTARRSWQLTLNNDIKLNLGRGDTMK
                     RLVRFVELYPVLQQQAQTDGKRISYVDLRYDSGAAVGWAPLPPEESTQQQNQAQAEQQ
                     "
     gene            complement(46011..46931)
                     /gene="ddlB"
                     /locus_tag="OO357_000866"
     CDS             complement(46011..46931)
                     /gene="ddlB"
                     /locus_tag="OO357_000866"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5885904.1"
                     /translation="MTDKIAVLLGGTSAEREVSLNSGAAVLAGLREGGIDAYPVDPKE
                     VDVTQLKSMGFQKVFIALHGRGGEDGTLQGMLELMGLPYTGSGVMASALSIDKLRSKL
                     LWQGAGLPVAPWVALTRAEFEKGLSDKQLAEISALGLPVIVKPSREGSSVGMSKVVAE
                     NALQDALRLAFQHDEEVLIEKWLSGPEFTVAILGEEILPSIRIQPSGTFYDYEAKYLS
                     DETQYFCPAGLEASQEANLQALVLKAWTTLGCKGWGRIDVMLDSDGQFYLLEANTSPG
                     MTSHSLVPMAARQAGMSFSQLVVRILELAD"
     gene            complement(46924..48399)
                     /gene="murC"
                     /locus_tag="OO357_000867"
     CDS             complement(46924..48399)
                     /gene="murC"
                     /locus_tag="OO357_000867"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HCR5885905.1"
                     /translation="MNTQQLAKLRSIVPEMRRVRHIHFVGIGGAGMGGIAEVLANEGY
                     QISGSDLAPNPVTQQLLNLGATIYFNHRPENVRDASVVVVSSAISADNPEIVAAHEAR
                     IPVIRRAEMLAELMRFRHGIAIAGTHGKTTTTAMVSSIYAEAGLDPTFVNGGLVKAAG
                     VHARLGHGRYLIAEADESDASFLHLQPMVAIVTNIEADHMDTYQGDFENLKQTFINFL
                     HNLPFYGRAVMCVDDPVIRELLPRVGRQTTTYGFSEDADVRVEDYQQIGPQGHFTLLR
                     QDKEPMCVTLNAPGRHNALNAAAAVAVATEEGIDDEAILRALESFQGTGRRFDFLGEF
                     PLEPVNGKSGTAMLVDDYGHHPTEVDATIKAARAGWPDKNLVMLFQPHRFTRTRDLYD
                     DFANVLTQVDTLLMLEVYPAGEAPIPGADSRSLCRTIRGRGKIDPILVPDPAQVAEML
                     APVLTGNDLILVQGAGNIGKIARSLAEIKLKPQTPEEEQHD"
     gene            complement(48453..49520)
                     /gene="murG"
                     /locus_tag="OO357_000868"
     CDS             complement(48453..49520)
                     /gene="murG"
                     /locus_tag="OO357_000868"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HCR5885906.1"
                     /translation="MSGQGKRLMVMAGGTGGHVFPGLAVAHYLMAQGWQVRWLGTADR
                     MEADLVPKHGIEIDFIRISGLRGKGIKALIAAPLRIFNAWRQARAIMKAYKPDVVLGM
                     GGYVSGPGGLAAWSLGIPVVLHEQNGIAGLTNKWLAKIATKVMQAFPGAFPNAEVVGN
                     PVRTDVLALPLPQQRLAGREGPVRVLVVGGSQGARILNQTMPQVAAKLGDSVTIWHQS
                     GKGSQQSVEQAYAEAGQPQHKVTEFIDDMAAAYAWADVVVCRSGALTVSEIAAAGLPA
                     LFVPFQHKDRQQYWNALPLEKAGAAKIIEQPQLSVDAVANTLAGWSRETLLTMAERAR
                     AASIPDATERVANEVSRAARA"
     gene            complement(49517..50761)
                     /gene="ftsW"
                     /locus_tag="OO357_000869"
     CDS             complement(49517..50761)
                     /gene="ftsW"
                     /locus_tag="OO357_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsW"
                     /protein_id="HCR5885907.1"
                     /translation="MRLSLPRLKMPRLPGFSILVWISTALKGWVMGSREKDTDSLIMY
                     DRTLLWLTFGLAAIGFIMVTSASMPIGQRLTNDPFFFAKRDGVYLILAFILAIITLRL
                     PMEFWQRYSATMLLGSIILLMIVLVVGSAVKGASRWIDLGLLRIQPAELTKLSLFCYI
                     ANYLVRKGDEVRNNLRGFLKPMGVILVLAVLLLAQPDLGTVVVLFVTTLAMLFLAGAK
                     LWQFIAIIGMGISAVVLLILAEPYRIRRVTAFWNPWEDPFGSGYQLTQSLMAFGRGEL
                     WGQGLGNSVQKLEYLPEAHTDFIFAIIGEELGYVGVVLALLMVFFVAFRAMSIGRKAL
                     EIDHRFSGFLACSIGIWFSFQALVNVGAAAGMLPTKGLTLPLISYGGSSLLIMSTAIM
                     MLLRIDYETRLEKAQAFVRGSR"
     gene            complement(50761..52077)
                     /gene="murD"
                     /locus_tag="OO357_000870"
     CDS             complement(50761..52077)
                     /gene="murD"
                     /locus_tag="OO357_000870"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HCR5885908.1"
                     /translation="MADYQGKNVVIIGLGLTGLSCVDFFLARGVTPRVMDTRMTPPGL
                     DKLPEAVERHTGSLNDEWLMAADLIVASPGIALAHPSLSAAADAGIEIVGDIELFCRE
                     AQAPIVAITGSNGKSTVTTLVGEMAKAAGVNVGVGGNIGLPALMLLDDECELYVLELS
                     SFQLETTSSLQAVAATILNVTEDHMDRYPFGLQQYRAAKLRIYENAKVCVVNADDALT
                     MPIRGADERCVSFGVNMGDYHLNHQQGETWLRVKGEKVLNVKEMKLSGQHNYTNALVA
                     LALADAAGLPRASSLKALTTFTGLPHRFEVVLEHNGVRWINDSKATNVGSTEAALNGL
                     HVDGTLHLLLGGDGKSADFSPLVRYLNGDNVRLYCFGRDGAQLAALRPEVAEQTETME
                     QAMRLLAPRVQPGDMVLLSPACASLDQFKNFEQRGNEFARLAKELG"
     gene            complement(52080..53162)
                     /gene="mraY"
                     /locus_tag="OO357_000871"
     CDS             complement(52080..53162)
                     /gene="mraY"
                     /locus_tag="OO357_000871"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HCR5885909.1"
                     /translation="MLVWLAEHLVKYYSGFNVFSYLTFRAIVSLLTALFISLWMGPRM
                     IAHLQKLSFGQVVRNDGPESHFSKRGTPTMGGIMILTAIVISVLLWAYPSNPYVWCVL
                     VVLVGYGVIGFVDDYRKVVRKDTKGLIARWKYFWMSVIALGVAFALYLAGKDTPATQL
                     VVPFFKDVMPQLGLFYILLAYFVIVGTGNAVNLTDGLDGLAIMPTVFVAGGFALVAWA
                     TGNMNFASYLHIPYLRHAGELVIVCTAIVGAGLGFLWFNTYPAQVFMGDVGSLALGGA
                     LGIIAVLLRQEFLLVIMGGVFVVETLSVILQVGSFKLRGQRIFRMAPIHHHYELKGWP
                     EPRVIVRFWIISLMLVLIGLATLKVR"
     gene            complement(53156..54514)
                     /gene="murF"
                     /locus_tag="OO357_000872"
     CDS             complement(53156..54514)
                     /gene="murF"
                     /locus_tag="OO357_000872"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HCR5885910.1"
                     /translation="MISVTLSQLTDILNGELQGADITLDAVTTDTRKLTPGCLFVALK
                     GERFDAHDFADQAKAGGAGALLVSRPLDIDLPQLIVKDTRLAFGELAAWVRQQVPALV
                     VALTGSSGKTSVKEMTAAILSQCGNTLYTAGNLNNDIGVPMTLLRLTPEYDYAVIELG
                     ANHQGEIAWTVSLTRPEAALVNNLAAAHLEGFGSLAGVAKAKGEIFSGLPENGIAIMN
                     ADNNDWLNWQSVIGSRKVWRFSPNAANSDFTATNIHVTSHGMEFTLQTPTGSVDVLLP
                     LPGRHNIANALAAAALSMSVGATLDAIKAGLANLKAVPGRLFPIQLAENQLLLDDSYN
                     ANVGSMTAAVQVLAEMPGYRVLVVGDMAELGAESEACHVQVGEAAKAAGIDRVLSMGK
                     QSHAISTASGVGEHFADKTALITRLKSLIAEQQVITILVKGSRSAAMEEVVRALQENG
                     TC"
     gene            complement(54511..55998)
                     /gene="murE"
                     /locus_tag="OO357_000873"
     CDS             complement(54511..55998)
                     /gene="murE"
                     /locus_tag="OO357_000873"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HCR5885911.1"
                     /translation="MADRNLRDLLAPWVPDAPSRALREMTLDSRVAAAGDLFVAVVGH
                     QADGRRYIPQAIAQGVAAIIAEAKDEATDGEIREMHGVPVIYLSQLNERLSALAGRFY
                     HEPSDNLRLVGVTGTNGKTTTTQLLAQWSQLLGETSAVMGTVGNGLLGKVIPTENTTG
                     SAVDVQHELAGLVDQGATFCAMEVSSHGLVQHRVAALKFPASVFTNLSRDHLDYHGDM
                     EHYEAAKWLLYSEHHCGQAIINADDEVGRRWLAKLPDAVAVSMEDHINPNCHGRWLKA
                     TEVNYHDSGATIRFSSSWGDGEIESHLMGAFNVSNLLLALATLLALGYPLADLLKTAA
                     RLQPVCGRMEVFTAPGKPTVVVDYAHTPDALEKALQAARLHCAGKLWCVFGCGGDRDK
                     GKRPLMGAIAEEFADVAVVTDDNPRTEEPRAIINDILAGMLDAGHAKVMEGRAEAVTC
                     AVMQAKENDVVLVAGKGHEDYQIVGNQRLDYSDRVTVARLLGGIA"
     gene            complement(55985..57751)
                     /gene="ftsI"
                     /locus_tag="OO357_000874"
     CDS             complement(55985..57751)
                     /gene="ftsI"
                     /locus_tag="OO357_000874"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000642204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase FtsI"
                     /protein_id="HCR5885912.1"
                     /translation="MKAAAKTQKPKRQEEHANFISWRFALLCGCILLALAFLLGRVAW
                     LQVISPDMLVKEGDMRSLRVQQVSTSRGMITDRSGRPLAVSVPVKAIWADPKEVHDAG
                     GISVGDRWKALANALNIPLDQLSARINANPKGRFIYLARQVNSDMADYIKKLKLPGIH
                     LREESRRYYPSGEVTAHLIGFTNVDSQGIEGVEKSFDKWLTGQPGERIVRKDRYGRVI
                     EDISSTDSQAAHNLALSIDERLQALVYRELNNAVAFNKAESGSAVLVDVNTGEVLAMA
                     NSPSYNPNNLSGTPKEAMRNRTITDVFEPGSTVKPMVVMTALQRGVVRENSVLNTIPY
                     RINGHEIKDVARYSELTLTGVLQKSSNVGVSKLALTMPSSVLVDTYSRFGLGKATNLG
                     LVGERSGLYPQKQRWSDIERATFSFGYGLMVTPLQLARVYATIGSYGIYRPLSITKVD
                     PPVPGERVFPESIVRTVVHMMESVALPGGGGVKAAIKGYRIAIKTGTAKKVGPDGRYI
                     NKYIAYTAGVAPASQPRFALVVVINDPQAGKYYGGAVSAPVFGAIMGGVLRTMNIEPD
                     ALTTGDKNEFVINQGEGTGGRS"
     gene            complement(57767..58132)
                     /gene="ftsL"
                     /locus_tag="OO357_000875"
     CDS             complement(57767..58132)
                     /gene="ftsL"
                     /locus_tag="OO357_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HCR5885913.1"
                     /translation="MISRVTEALSKVKGSMGSHERHALPGVIGDDLLRFGKLPLCLFI
                     CIILTAVTVVTTAHHTRLLTAQREQLVLERDALDIEWRNLILEENALGDHSRVERIAT
                     EKLQMQHVDPSQENIVVQK"
     gene            complement(58129..59070)
                     /gene="rsmH"
                     /locus_tag="OO357_000876"
     CDS             complement(58129..59070)
                     /gene="rsmH"
                     /locus_tag="OO357_000876"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HCR5885914.1"
                     /translation="MMENYKHTTVLLDEAVNGLNIRPDGIYIDGTFGRGGHSRLILSQ
                     LGEEGRLLAIDRDPQAIAVAKTIDDPRFSIIHGPFSALGEYVAERDLIGKIDGILLDL
                     GVSSPQLDDAERGFSFMRDGPLDMRMDPTRGQSAAEWLQTAEEADIAWVLKTYGEEHF
                     AKRIARAIVERNREQPMTRTKELAEVVAAATPVKDKFKHPATRTFQAVRIWVNSELEE
                     IEQALKSSLNVLAPGGRLSIISFHSLEDRIVKRFMRENSRGPQVPAGLPMTEEQLKKL
                     GGRQLRALGKLMPGEEEVAENPRARSSVLRIAERTNA"
     gene            complement(59072..59530)
                     /gene="mraZ"
                     /locus_tag="OO357_000877"
     CDS             complement(59072..59530)
                     /gene="mraZ"
                     /locus_tag="OO357_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020325403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HCR5885915.1"
                     /translation="MFRGATLVNLDSKGRLSVPTRYREQLLENAAGQMVCTIDIHHPC
                     LLLYPLPEWEIIEQKLSRLLSMNPVERRVQRLLLGHASECQMDGAGRLLIAPVLRQHA
                     GLTKEVMLVGQFNKFELWDETTWHQQVKEDIDAEQLATGDLSERLQDLSL"
BASE COUNT        14355 a        16652 c        14777 g        14285 t
ORIGIN      
        1 ggtgcggtaa ttgataaatc attgccccca ccaatgtgac tgataaaatg ccaggcaaca
       61 cgacatcatt tatcaggata cgttatgaaa ccgaccacca ttgcttcact tcagaaatat
      121 aagcaggata aaaagcgttt cgcgaccatc accgcttacg actacagctt cgcgaaacta
      181 tttgctgatg aagggcttaa cgtcatgctg gtaggcgact cgctgggcat gaccgttcag
      241 ggccacgatt ccactctgcc agtaaccgtt gctgatatcg cctaccacac tgccgccgta
      301 cgtcgcggcg caccaaactg cctgctattg gctgacctgc cgtttatggc gtatgccacg
      361 ccggaacaag cctttgaaaa cgccgcaacg gttatgcgtg ccggtgccaa tatggtcaaa
      421 attgagggcg gtgagtggct ggtcgaaacc gtaaaaatgc tgaccgaacg tgccgttcct
      481 gtatgtggtc acttaggttt aaccccacag tcagtgaata ttttcggtgg ctacaaagta
      541 caggggcgcg gcaatgaagc gagcgatcga ctactcagcg atgcattagc cttagaagcc
      601 gcgggggcac agctgctggt gctggaatgc gtgccggttg agctggcaaa acgtattacc
      661 gaagcactgg cgatcccggt tattggtatt ggtgcaggca acgtcactga cgggcaaatc
      721 ctcgtgatgc acgacgcctt tggtattacc ggcggtcaca ttcctaaatt cgctaaaaat
      781 ttcctcgccg aaacgggcga catccgcgcg gctgtgcggc agtatatggc tgaagtggag
      841 tccggcgttt atccgggcga agaacacagt ttccattaag gagtcacgtt gtgttaatta
      901 tcgaaaccct gccgctgctg cgtcagcaaa ttcgccgcct gcgtatggaa ggcaagcgcg
      961 tggcgctggt gcctactatg ggtaatctgc acaatggcca tatgaagctg gtcgacgaag
     1021 ccaaagcccg cgccgatgtg gtcgtcgtca gtattttcgt taacccgatg cagttcgacc
     1081 gcccggaaga tctggcacgt tatccacgga ccttgcagga ggactgcgag aagctaaaca
     1141 aacgtaaagt ggatttagtt ttcgcccctt cggtaaaaga gatctacccg aacggtactg
     1201 aaacccacac ttacgttgac gttcctggcc tttcgaccat gctggaaggt gccagccgtc
     1261 cgggacattt tcgcggcgtt tcgactattg tcagcaagct gttcaacctg gtccagccgg
     1321 acatcgcctg cttcggtgaa aaagattttc agcaactggc gttgatccgc aaaatggttg
     1381 ccgatatggg cttcgatatt gagattgtcg gtgtgccaat tatgcgcgcc aaagacggtc
     1441 tggcgctaag ttcccgtaac ggttatctga cggcggaaca acgcaaaatt gcgcctggtc
     1501 tgtacaaagt tttaagttcg attgctgaca aattgcaggc tggggaacgg gatctcgatg
     1561 aaattattac cattgcgggg caagaactga atgaaaaagg cttccgcgcc gatgatattc
     1621 agattcgcga tgccgacaca ttgctggaag tttctgaaac cagcaaacgg gcagtaattc
     1681 tggtagccgc ctggcttggc gatgctcgcc tgatcgacaa caaaatggtc gagctggcgt
     1741 aatacttaac tggcgctacg gctgatggcg ccagttatta atttacccca cgtcatctgt
     1801 cataacacag gtaacttaaa taaggttaat taccttatca atctcagcaa gaggtaaatt
     1861 tgccatctct gcaacgtctt cccgagacat tcctttactt agaagacgca gggcgaattc
     1921 ttgcctttcc tcctgtcttc cctgctgaat ccccttctca atccctttct cttcaaacca
     1981 ctgcgccagc gtcatcatag acttccctcc cgtttccctg tctctcagca caccataaaa
     2041 caaatccgct tgttcagtat gaccgcgttg cagcatatag ttttgcatgg caactaactg
     2101 acttccgcta gtgtaccctt cgtcaatcag cgtgaccagt tgctccagca ataacattaa
     2161 gtcgcgctgg cgaatatgtt tttgcagtag ttcgagaatc gcaatccgcc gatgttgcat
     2221 gatttcgtca tccggtgtga tggtgatatc caccagcggg aaaggactgt tatagacgcg
     2281 tcgcgccagc tccggcgagt aaaacatatc aaaccagcac attgagagcg gataaggtgt
     2341 ggcctcgccc tgataaaaca gtatcggcac caccagcggc agcttatcgt ggccagcctc
     2401 cagatgccgg tgcatggcag caatagaata acgcatcatg cgaaaggcca ttttcttatc
     2461 cggcttgctt tggtgttcaa tcacaacatg cagataaccg ggattgccct gcatttgcat
     2521 ggaatagagc acgtccgtgc tgtgaccttt caggcactct tcaatgaaac tccccgactc
     2581 taaatgaagc gtgttgagat cacaaagttc gcgtaattcc actggcaaat gtatctccag
     2641 aaagtcgcga gccgtctccg catgcattaa aaattgttta aataccgcgt catgcggtgt
     2701 ggtactcggt gcatccatct tccctgtctt ccgtgtggcc ttgcgtgaag gcagtatcca
     2761 gtcaaggcgt gctatcgtca gccacctctt tattgtttac cgagcagcgt tcataaaaat
     2821 atcaattcgt tacaggcgtt acatggcacg cttcggcgcg gtacgcaaac tgctgacgca
     2881 aatgccatca gatgagtaga cactcatcaa aaatcgggca atactgcgtg agaatttcct
     2941 caaagcaggc accgcctgct tcgttaacga cagggtagaa aggtagaagt tatgattcgc
     3001 acgatgctgc agggcaaact ccaccgcgtg aaagtgactc atgcggacct gcactatgaa
     3061 ggttcttgcg ccattgacca ggattttctt gacgcagccg gtattctcga aaacgaagcc
     3121 atcgatatct ggaatgtcac caacggcaag cgtttctcca cttatgccat cgcggcagaa
     3181 cgcggttcga gaattatttc tgttaacggt gcggcggccc actgcgccag cgtcggcgat
     3241 attgtcatca tcgccagctt cgttaccatg ccagatgaag aagctcgcac ctggcgaccc
     3301 aatgtcgcct attttgaagg cgacaatgaa atgaaacgta ccgcgaaagc gattccggta
     3361 caggttgctt gattgttatc cctgcggctg gttactcacc agccgcgaca tcttctccag
     3421 cgaatccgtt cttaagatat aaagtcgttt tagtaacaac ggattatccc ccggttttac
     3481 tttgcctttc atggttgtca ccgccaggtg aaatccggca tcgtttgctg ccttcacggc
     3541 tttgtcatta aagccgccaa acggatacga aagataccag acatgtggat taaattgcgc
     3601 cagagcgcgg cgtgaacgtg caaaatcaaa cagaatattg tgctcactac ggctcagtaa
     3661 tatgggtcgg cgataaccat ctacccgatg caaaaaatgg gtatgtgact ggaaatcaaa
     3721 aacatcgcga atttcgttaa gctcagaaac gctcataaat tgcagcgatt ttgggttcca
     3781 cttctgcggg tgacgtttga tgcgtgaggt aacaataaac accgtcgcct tcatgccata
     3841 ttgtttcaac acaggatacg catagcggct caccgacttg aggccatcat caaaggtaat
     3901 caccaccgct cgcgcaggga gattgatcgt attcttcacg taaccttcca gctgagccat
     3961 gctcagtgtc gcgtatcccc tgtcgcgcag ccaggccatt tggttattga aagcgcgtac
     4021 cgaggtggtc gtcgaagtat ggcgaaaacg ggtgttttct tcgtcgcgca gaatatggtg
     4081 ataagttagc accggcaggc cattatcggg ttgggcatcc agcgcgctga tataggccag
     4141 tcgatcgcca atacggatct gataccaggt ttgatttaac ctgtctttca gtttatgcaa
     4201 aatcgggtag cgcaaattgt ccgccagtac cccaaatggc gcactgccca cactcggcgc
     4261 gttgtagacc ggcgtatctt tccaggtaat taagttctga ttactcagcg gcttgttgag
     4321 gtcacccaaa ccgtcttcaa ctttttgtcg tccctgaact ggctcgagat gccctttatc
     4381 gataaaccct ttaccaaagc caaaattaaa tgcgtaataa cttgcggcag tgggctccac
     4441 ggcaatgatt tgtccggccc gaatattccc cacggtcacc atcttgtcac taatttgcgc
     4501 ccagaccgca gcattttcga tggtttgcat ataacgggca ggtaacgcgg cactgacact
     4561 tgcggtaaac agcatcagca ggagaataac agcttgttta tacatgaaaa aataaccgag
     4621 ggtgaaacaa cgcttttatt gtagcaaaag cgccatcaga atcgcgggtt aatactcata
     4681 caaattatgt ttgaggacga aaggcggatt tttttgttgt tggtgccaaa gactactcac
     4741 ctccggccca cccagttcga caatacactc acgaaactct gaactggtca tggtttcacg
     4801 gcaagccatc atctgttcaa ttaacggtaa cgtgacacca gaaatcactt cgcaacggga
     4861 gtgtttgtgg cttaataatg aagccacgcg atacggtggc gctccggcta tatcggttaa
     4921 gaagatgaca ccctcaccag agtccgcttc atgcagagca tcgcacatca tgcggctgag
     4981 catattagag cttaatccgc gccagaaatt cacggcccgg cactgaagaa gtgccccatg
     5041 ttttttctcc agcgcatcca gtatctcttg cgcccggtca tcgtgacagg taattaccca
     5101 acctaacatc gccttcctcc ttagccgaaa atcagcctac cctgaccatt gttgataaag
     5161 ggtgatagcc gtcaaaactc ctctcccccg aatccagagg agaggaaata gccttagcta
     5221 cgcaaaccac gtccacgttg gatcagcgac caacagatca aataaaacgc cacaataaag
     5281 accaccagta cgccaaaggt agtgaccagc ggcacatcat ttataccgag gaagccgtag
     5341 cggaaaccac tgatcatata aacgattggg ttcaggtgcg acagcccttg ccagaacggc
     5401 ggcaacaaag tcagtgagta aaagaccccg cccaaatacg tgagtggcgt taacacaaag
     5461 gttggcacca ggctgatgtc atcgaacgtt ttggcaaaca cgccgttcag caaacccgca
     5521 agggagaata gcaccgccgt gagcaccagg gttaaggcaa cgaataccca cgaatgcacc
     5581 tgaaacggca caaaaaacag tgaaattgcc gtcacaagaa tgccaacaaa cagaccacgc
     5641 gccacgccac cgccgacata tccggcaata atgacgtgag tcggaaccgg cgctaccagc
     5701 agctcttcaa tattacgctg gaacttggca ccaaaaaatg atgacgcgac gttagcgtag
     5761 gcgttggtga tcaccgacat catgatcagc cccggtacga tgaactgcat atagctgaag
     5821 ccatgcatat cgccaatacg cgaacctatc aggttaccga agataataaa gtaaagggtc
     5881 atggtgatga ctggcggcac cagcgtctgc acccagatac gcataaagcg atggatctct
     5941 ttcgcccaga tgctttttag cgccacccag taaagatgca tcatgcgcga tctccttgtt
     6001 tttcattaac cagtgaaaca aacagctctt ccagacggtt agctttgtta cgcatactta
     6061 atacctgaat gccctgctca cttaactgcg taaatacgct gttgatcccc tgctcacgta
     6121 gcacttcaac ttccagcgtc gcggtatcga ccagtcgata ctgatagcca tcgagcttcg
     6181 gtaacggact tttcggtgcg agatcgagaa taaaggtttc cgatttcagc ttcgccagca
     6241 gcgccttcat cgaggtattt tccaccagct caccatgttg aataatgccg atattgcggc
     6301 acagcatttc tgcttcttcc aggtagtgtg tggtaagaat gatggtggtg cctttgtcgt
     6361 ttaaatcctt caaaaagccc cacattgagc ggcgaagttc aatatccacg cctgcggtcg
     6421 gttcgtcgag aatcagtagt ttaggttcat gcattaacgc acgggcaatc attaaacggc
     6481 gcttcatccc gccagataac atacgcgcac gttcgttgcg ttttccccat aaatcgagct
     6541 gcttaagata tttttcgctg cggatgtacg cctctttgcg ctccacaccg tagtaccctg
     6601 cctgattcac cacaatttgc tgcacggttt caaacgggtt gaagttaaat tcctgcggca
     6661 ccagtcccaa ctgacgttta gcgttcacga catctttctc gagatcgtaa ccaaatacgc
     6721 tgacccgccc ggaggtttta tttaccagag agctgataat accgatagtg gtcgatttcc
     6781 cggccccgtt cggcccgaga agcgcataaa aatcacccgc ttcgacctgc aaatctattc
     6841 cacgaagcgc ctgaacgccg cctggatagt tttttttaag ctgttgaagt tccagtgcaa
     6901 tggtcataaa tttttactta ccttacgttc ttacacttta tctgtggttt aaatcgtccc
     6961 ggagttgccc tatattagcc aaacgtaatt atttggttac aggtcgttaa cctccatgaa
     7021 agacatagat acactcatca gcaacaatgc actatggtca aaaatgctgg tggaagagga
     7081 tcccgggttt tttgagaaac tggcacaagc gcaaaaaccg cgctttctat ggattggatg
     7141 ttccgacagt cgcgttcctg cagaacgttt aaccggtctt gagccgggcg aactctttgt
     7201 tcaccgtaat gttgctaacc tagtcattca caccgacctg aactgccttt ccgtggttca
     7261 gtatgcagtg gatgtactcg aagttgaaca cattattatc tgtggccact acggttgcgg
     7321 cggcgtacaa gccgccgttg aaaacccgga actggggctt atcaacaact ggctgctgca
     7381 tatccgcgat atctggttca aacatagctc attgctcggc gaaatgccgc aagagcgccg
     7441 tctggatacc ttgtgtgaac tgaacgtcat ggaacaggtg tataacctgg gccactccac
     7501 cattatgcaa tcagcgtgga aacgcgggca gaaagttacc attcacggct gggcctacgg
     7561 cattcacgac ggcttgctgc gtgatctgga tgttaccgcc accaaccgcg aaacccttga
     7621 gcaacgttac cgtcacggga tttccaacct caagctgaaa cacgccaacc acaaataaaa
     7681 atgccatgcc ggatgcaaca catccggcaa cttcacactt actcgtccag cagaatcact
     7741 ttgccaatat acggcagatg acggtaacgc tgtgcgtaat caatgccgta acccaccaca
     7801 aactcatccg ggatcgagaa accgataaat tcaaccggga cgttcacttc acgacgggac
     7861 ggtttatcca gcagcgtaca aatcgccaac gacttcggtt cgcgcaggct taagatctca
     7921 cgcactttcg acagtgtatt ccccgagtcg atgatatctt caacaatcag cacgtccttg
     7981 ccacggatat cttcatccag atctttgagg attttcacat cacgggtggt ggacatgccg
     8041 ctaccgtagc tggaggcggt cataaagtcg acttcatgag atacctgaac ttcacggcac
     8101 aggtccgcca taaacataaa tgagccacgc agcagaccca ccagcaccat atcgctgccg
     8161 ctgtctttgt aacgctcagt aatctgacga cccagttcgg cgatacgcgc tttaatctcc
     8221 gcttcgggga tcattacttc tacagtatgt ttcatatctc taaccatatg atttaaaaac
     8281 aaatcacttc gcacgggcag gttttggcac cgcaacaaag cgcaagacgc gcagtatacc
     8341 agtaaaacta ttcattcgcg gaatctgtta ataaagctcg aattgtgatg acggtcacac
     8401 atgttaaaac ctataattaa ttgctactaa aatattaatg aattgaaatg gtgtctcttt
     8461 atggcaatta acaatacagc ctcgcgacga ttactcgtca cgctaacagc cctttttgca
     8521 gcgctttgcg ggctgtatct actcattggc ggaggctggc tggtcgcgat tggcggctcc
     8581 tggtactacc ctatcgctgg ccttgtgatg ctcggcgtcg cctggatgct gtggcgcagt
     8641 aaacgcgccg cgctttggct atacgcagcc ctgctgctcg gcaccatgat ttggggcgtc
     8701 tgggaagttg gtttcgactt ctgggcgctg actccgcgca gcgatattct ggtcttcttc
     8761 ggcatctggc tgatcctgcc gtttgtctgg cgtcgcctgg tcattcctgc cagcggcgca
     8821 gttgccgcac tggtggtcgc actgctgatt agcggtggta tcctgacctg ggccggattt
     8881 aacgatccgc aggagatcaa cggcacctta agcgccgatg ccacacctgc cgaagctatc
     8941 tcccccgttg ccgatcagga ctggcctgcg tatggccgca atcaggaagg tcaacgcttt
     9001 tcaccactga agcaaattca cgccgataac gtccacaagc tgaaagaagc ctaggtgttc
     9061 cgtactggcg atgtgaagca gccgaacgat ccgggtgaaa tcaccaatga agtgacgccg
     9121 attaaagtgg gcgataccct ttacctgtgt accgctcacc agcgcctgtt tgcgcttgat
     9181 gccgccagcg gcaaagagaa atggcattac gatcctgagc tgaaaaccaa cgagtctttc
     9241 cagcacgtaa cctgccgtgg cgtctcttat catgaagcca aagcagaaac ggcttcgccg
     9301 gaagtgatgg cggattgccc gcgtcgtatc attcttccag tgaatgatgg tcgcctgatt
     9361 gcgattaacg ctgaaaacgg caaactgtgc gaaaccttcg ccaataaagg cgtgctcaat
     9421 ctgcaaagca atatgccaga caccaaaccg ggtctgtatg agccgacttc tccgccaatt
     9481 atcaccgata aaaccatcgt gatggccggt tcagtcaccg ataacttctc aacccgcgaa
     9541 acgtctggcg tgatccgtgg ttttgatgtc aacaccgggg agctgctgtg ggcttttgat
     9601 ccgggcgcga aagatccgaa cgcaatcccg tcagacgaac acacctttac ctttaactca
     9661 ccaaactcgt gggcaccagc ggcctatgac gcgaagctgg atctggtcta tctgccgatg
     9721 ggcgtgacca cgccggatat ctggggcggt aaccgcacac cggaacagga acgttatgcc
     9781 agctcgattc tggcgctgaa tgccactacc gggaaactgg cgtggagcta ccagaccgtt
     9841 caccacgacc tgtgggacat ggatcttccg gcacagccga cgctggcgga catcaccgtt
     9901 aatggtcaga aagtgccgat tatttacgct ccggcgaaaa ccggtaacat ttttgtgctc
     9961 gatcgtcgta atggcgaact ggtggttccg gccccggaaa aaccggttcc ccaaggtgct
    10021 gccaaaggcg attacgttac ccctactcaa ccattctcgg aactgagctt ccgtccgacg
    10081 aaagatttga gcggtgcgga tatgtgggga gccaccatgt ttgaccaact ggtgtgccgc
    10141 gtgatgttcc accagatgcg ctatgaaggc attttcacgc cgccatctga acagggtacg
    10201 ctggtcttcc cgggtaacct ggggatgttc gaatggggcg ggatttccgt tgatccaaat
    10261 cgtgaagtgg caattgccaa cccaatggca ctgccgtttg tttcgaaact gctcccgcgt
    10321 ggtcctggca acccgatgga gcagccgaaa gatgccaaag gcacgggtac ggaatccggc
    10381 attcagccac agtacggtgt accgtatggt gtcacgctca acccgttcct ctcaccattt
    10441 ggtctgccat gtaaacagcc agcatggggt tatatctcgg cgctggatct gaaaactaat
    10501 gaagtggtgt ggaagaaacg tattggtacg ccgcaggaca gtatgccgtt cccgatgccg
    10561 gttccggtgc cgttcaatat gggtatgccg atgctgggcg ggccaatctc cacggcgggt
    10621 aacgtgctgt ttatcgccgc tacggcagat aactacctgc gcgcttacaa catgagcaac
    10681 ggtgaaaaac tgtggcaggg tcgtttaccg gcgggtggtc aggcaacacc gatgacctat
    10741 gaagtgaatg gcaagcagta cgtggtgatc tccgcaggcg gtcacggttc atttggtacg
    10801 aagatgggcg actatattgt ggcttatgcc ctgccggatg atgtgaaata accataatgc
    10861 ccggagagat ccgggcatat ttccgaatac ggtcttttta taccgtaaac cctaacatca
    10921 tccccgtatc ttcatgctcc agcagatggc agtgcgccat ataagcatgt tctttcggtg
    10981 cgttgtgatt aaacttcacc agcacttcac tgacattgcc ttctacctta acggtatctt
    11041 tccagcccgc gcgatgagcc gctggcggtt tgccattttc tgacaagata cggaactgcg
    11101 tgccgtgaat atggaacgga tgcagcatca tgtcgcccac gccagagata acccaacgtt
    11161 cgtactgccc tttcgccgcc gcaaacatcg gcttattcat atcaaacgcc tgaccgttga
    11221 ttttattggc atggtggaaa tcgaacttcc cgccgtggtt catatgattc atattgccgt
    11281 gccccatatg gcccatcatc tggctgtgat ccattccggc catcgcctga tcgccatatt
    11341 tctccattag catctgcatc cccatcatat cgagcatcgg gtccatagaa agttgcagct
    11401 tgcgtaccgt cagcccttcc agcgaaggta acgcaggcag gctacttaat gtgtcaggca
    11461 aagcaccgga ggcactaata gcaatcggct gaatccgcat taccggatgc ggcttgtcaa
    11521 acggtgcaat cgccatcccc atctggctga ccggcagcgt caccaggtca aagggtttgt
    11581 tgtcgttaac ctccaccagc acttcaaaac gctcgcccat cagcaccggc agctcgctca
    11641 ccttcaccgg ttcaggtagc agaccaccgt cgctggcaat cacatacagc gggcgattgt
    11701 cgctggtggc gaaattgagc gagcgggcat tacagccatt gagcaaacgc aggcgcagcc
    11761 aaccacgcgg ggcagcgtgt tgtgggtaga ttgcaccgtt ggtcagcagc gtatcgccaa
    11821 accagcccac ggcggcggtc atcacatcca gttgataatc aatctgcccg tcggcgctaa
    11881 atttcttatc ctgaacgatc accggaacat catcgatacc ccactgtttt ggcagcatta
    11941 atttcaggat ctcgtcatct tcaatcacca ccagaccagc cagccccatc gccacctgtc
    12001 gcccggtctt gccatgttga tgtggatgga accagcaggt agcggcaggt tgatcaacgt
    12061 tcaacgtcac cgagcgcttg ccacctggcg gaataattcc ctgtgggccg ccgtcgacgt
    12121 cacccggtac ttccagcccg tgccagtgca acgtcgtctc ttccgccagt tggttgtaga
    12181 tatcaaccgt taccgctttg ccgcgctgta atttcaccgc cggccccagc agattgccgt
    12241 tatagcccca ggtagttgca gttttctcgc caaaggtgga ctgacctgcg ccaatagtta
    12301 actgaatgcg attacgggca tcggtcgtga gcaaatcagg gatcggtaac gttgggcgtt
    12361 ccgccgcaaa gactgcacgg ctccacagtg gcaaggctga agccacaccc agtgcgacgg
    12421 aatatttcaa gaaatcacga cgttgcatag ttatttcctt attcttaagc aggcgaaaca
    12481 aaatcaaacg ttgagcatag tccttcccct tacgggaagg tcaagccgca gacacaattt
    12541 aatcgacaat aatggtcgtc gcctcgctcg cagtgtgcta acgtttatct tctttaagcc
    12601 cctggtagaa gcaatgaaga cgtttttcag aacagtgtta ttcggcagcc tgatcgccgt
    12661 ttgcgcaaac agttacgcgc tcagcgagtc tgaagccgaa gatatggccg atttaacggc
    12721 agtttttgtc tttctgaaga acgattgtgg ttaccagaac ttatctaacg ggcaaattcg
    12781 tcgcgcactg gtctttttcg cccagcaaaa ccagtgggat ctcagtaatt acgacacctt
    12841 cgacatgaaa gctctcggtg aagacagcta ccgcgatctc agcggcattg gcattcccgt
    12901 cgctaaaaaa tgcaaagccc tggcccgcga ttccttaagc ctgcttgcct acgtcaaata
    12961 atccctgata cctttttgta gaaatattga ccgtgcatcc gcggtcaatg ttagctatta
    13021 tgttgcgccc tttttttacg ggtgttaaca aaggaggtat caacccatgg ccgaaaaaaa
    13081 acagtggcat gaaacgctac acgaccagtt tgggcagtac tttgcggtag ataacgttct
    13141 gtatcatgaa aagaccgatc accaggatct gatcattttt gagaacgctg catttggtcg
    13201 cgtaatggcg ctggatggcg tagtacaaac caccgagcgc gacgagttta tctatcatga
    13261 gatgatgacc catgttccgc tactggccca cggtcacgcg aaacatgtgc tgattatcgg
    13321 cggcggcgac ggtgccatgc tgcgtgaagt aacccgacat aaaaacgttg agtcaatcac
    13381 gatggtggaa atcgatgcgg gtgtcgtgtc gttctgccgt cagtatctgc ccaaccataa
    13441 cgccggtagc tacgacgatc cgcgctttca actagtgatc gacgatggcg tcaatttcgt
    13501 taatcaaacc agccagacct ttgatgtcat tatctccgac tgcaccgatc ctatcggtcc
    13561 cggcgaaagc ctgttcacct cagcatttta tgaaggctgc aaacgttgcc tgaatcctgg
    13621 cggtatcttc gtcgcacaaa acggcgtctg ctttttacag caggaagaag ccatcgacag
    13681 ccatcgcaaa ctcagccatt acttcagcga cgttggcttt tatcaggcgg cgatcccgac
    13741 ctattacggc ggtatcatga cttttgcatg ggcgacagat aacgacgcct tacgccatct
    13801 ctcaaccgaa attattcagg cgcgttttct cgcttctggc ctgaaatgcc gttattacaa
    13861 tccggcaatc catacggcag cttttgcctt acctcagtat ctgcaagacg cactggcttc
    13921 acagccgtcc taaggagaag ataagaaatt gaaaaaactg aaactgcatg gctttaataa
    13981 tctgaccaaa agtctgagtt tttgtattta cgatatctgc tacgccaaaa ctgccgaaga
    14041 gcgcgacggt tatattgctt atatcgatga actctataat gccaaccgtc tgaccgaaat
    14101 cctgtcagaa acctgttcca ttatcggggc caatattctt aacatcgccc gccaggatta
    14161 cgaaccacag ggtgccagcg tcactattct ggtgagtgaa gaaccggttg acccgaaact
    14221 catcgacaaa acagaacacc ccggcccact gccagaaacg gtggttgctc atctcgataa
    14281 aagtcatatt tgcgtacata cctacccgga aagtcatcct gaaggcggtt tatgtacctt
    14341 ccgcgccgat attgaagtct ctacctgcgg cgtgatttct ccgctgaagg cgctgaatta
    14401 cctgatccac cagcttgagt ccgatatcgt aaccattgat tatcgcgtgc gcggttttac
    14461 tcgcgacatt aacggtatga agcactttat cgaccatgag attaattcga ttcagaactt
    14521 tatgtctgac gatatgaagg cgctgtatga catggtggat gtaaacgtct atcaggaaaa
    14581 tatcttccat accaagatgt tgcttaaaga gttcgacctt aagcactaca tgttccacac
    14641 caaaccggaa gatttaaccg acagcgagcg ccaggaaatt accgctgcgc tgtggaaaga
    14701 aatgcgcgag atttattacg ggcgcaatat gccagctgtt taacggctct ggcggagctc
    14761 ccaggctccg ccagatttat ttacttctgc tgcacgaaat tgcggtaagc cgccacgacc
    14821 tgcagaaaat cctcaacgcc gcatagcgac aggctttctt cgtcgtagta gttcatcccc
    14881 tcttccattt catcgccagc gaattccagt tgattggcgc gaaccatcac ctcttcagcg
    14941 tccatccaca gggtgtattc atgccctgcc cgttgccagg accgttcgct acctttcagt
    15001 gcgtgcgctg cttgttccac ttcatcaagc aaggccaggt tttctttcac ctcttcatta
    15061 aaccagtgcc cgaccacttc atgccccatg gacatacgca cctttaccac tccggtaata
    15121 tcgcgcagaa attcgtaatc catagtgtgt tcctctgctc ccggcaatgc gccgaaatcg
    15181 catcgcgcca ttgatgtaat tatcgcagcc ttaccggaga aaaacagcgg aacggcgata
    15241 gtttagaaat aaaaaatgcc cgggaatctc attcaccggg cattgtgtcg tttatgcgca
    15301 gcgcgtgcgc ttacttatta caccgcagtc tggaaaatca ccccatcggc tttttcggtg
    15361 tactgagaaa gctggttgaa gttcagataa cgataagtat caacggcagt tttatctact
    15421 tgcgcaacgt aggtctggta ctcttccggc gtcggcagtt tgccaatcag cgccgcaaca
    15481 gccgccagtt ctgcagaagc caggaagaca ttcgcgccag tacccaaacg gttcgggaag
    15541 ttacgggtag aggtggaaac caccgtcgca ccgtctgcta cacgcgcctg gttacccata
    15601 cacagggaac agcccgggat ctcgatacga gccccgctct taccgaagac gctgtagtag
    15661 ccttcttccg tcagttgcgc ggcgtccata cgggttggcg gtgccaccca caggcgggtc
    15721 ggcaactgac ctttgtgcgc atccagcagt ttaccagcgg cacggaagtg accgatgtta
    15781 gtcatgcaag aaccgataaa tacttcgtcg atcttctcgc cctgtaccgc agacagcgga
    15841 cgtgcgtcat ccggatcgtt cggagcacac aggattggct ctttaatctc cgccagatcg
    15901 atgtcgatca ctgccgcgta ttccgcatct gcatcggctt ccagcagctc aggattcgcc
    15961 agccattttt ccatgccctg aatacggcgt tccagggtac gacgatcgcc gtaaccttcc
    16021 gcgatcatcc acttcagcag gacgatgttg gagctcaggt attcgatgat cggttctttg
    16081 ttcagcttga tagtacaacc agcggcagaa cgctcggcgg acgcatcggt tagctcaaag
    16141 gcctgctcaa ctttcagatc cggcagacct tcaatttcca gaatgcgacc agagaagatg
    16201 tttttcttgc ctttcttctc aacggtcagc agaccttgtt tgatcgcata cagcggaatg
    16261 gcgtgtacca gatcgcgcag ggtgatgccc ggctgcattt tgcctttgaa gcgcaccaga
    16321 acggattccg gcatatccag cggcattacg ccagttgcgg cagcaaacgc caccagacca
    16381 gaacccgccg ggaaagagat accgatcggg aaacgggtat gggagtcacc accggtaccg
    16441 acggtatccg gcagcagcat acggttcagc caggagtgaa tgacgccgtc acccggacgc
    16501 agcgacacac cgccacggtt cataatgaag tccggcagcg tgtggtgcgt gttcacgtca
    16561 actggcttcg gatacgccgc ggtgtggcag aaagactgca tcaccaggtc agccgagaag
    16621 cccaggcacg ccaggtcttt cagttcatca cgggtcatcg ggccggtggt gtcctgagag
    16681 cctacagaag tcattttcgg ttcgcagtac gcgcccggac gaatgccttt cacgccacag
    16741 gcacggccta ccattttctg cgccagcgag aagccgcgat cgctctcagc gacatctttc
    16801 gcctgacgga acacatcact gtgcggcaga ccaagtgctt cacgcgcttt ggtggtcagg
    16861 ccacgcccga taatcagcgg aatacggccg ccagcacgca cttcatcaat cagcacgtcg
    16921 gttttcagtt cgaaggtcgc cagcagttcg cctgtttcgt ggttacgcac ttcacctttg
    16981 tacgggtaaa cgtcaatcac gtcgcccatg ttcaggttag agacgctgac ttcgattggc
    17041 agtgcacccg cgtcttccat cgtgttaaag aagatgggtg caattttacc gccgaggcac
    17101 aaaccaccgc cgcgtttgtt cggcacatgt ggaatatcat cgcccataaa ccacagaacg
    17161 gagttcgtgg cggatttacg cgatgaaccc gtacccacaa cgtcaccgac gtacgccagc
    17221 gggaaacctt tctgttgcag agcttcaatc tgtttgatcg ggccaacaac acctggctgg
    17281 tctggctcaa taccttcacg ggcgtttttc agcatcgcca gcgcgtgcag tgggatatcc
    17341 gggcgtgacc acgcatccgg tgccggagaa aggtcatcgg tgttggtttc gccagtgact
    17401 ttaaagacgg taacggtcag tttttcagcc agcgccgggc gattcaggaa ccattcggca
    17461 tccgcccagg actgcattac ctgcttcgca tattcgttgc ctgctttcgc tttctcttct
    17521 acgtcataga agttatcgaa catcagcagc gtgtgagaaa gtgctttggc agcaataggt
    17581 gccagtttgg catcatccag cgcgtcgatc agcggatgaa tgttgtaacc accctgcatg
    17641 gtgcccagca gttcgatagc tttttccgga gtcagcagag gggatttagc ttcgccttta
    17701 gcgatagcag ccaggaagcc tgcttttaca taggcggctt catcgacgcc tgggggaaca
    17761 cggttggtta acagatctaa caggaattct tcttcgcccg cgggcgggtt tttcagcagc
    17821 tctacaagcg cggccatttg gtttgcatcc aggggtttgg gcgcaatccc ctcagcggca
    17881 cgctcagcta cgtgcttacg gtattcttct agcacgacgg ttctcctcgc tctcattgtc
    17941 atagtgcggc aggcgattct cttcacgctc ctgtgagaca gcagtttgta gggtaaatgc
    18001 ccagtaccgc aacgggcaga atagcaggat tttgacgacg tgttaatctg tttacaaaaa
    18061 agcaacataa aaaagtggct gaatcgttaa ggatggtcta gagattgttt ccctccgtaa
    18121 aaacttcgtt aattcgcatg gtgataatca cctttctcaa cgaatcccaa gattagtcaa
    18181 aatttaaact accgcctctt tatactcgga ttcacagcac ctgcgggtgg cagttcgccg
    18241 accattgcga tttccttgag atccgaatta tgaaaatgac tttgccgttt aaaccccatg
    18301 tgctggcgct aatttgcagt gccgggcttt gtgctgcctc tgccgggcta tatataaaaa
    18361 gccgcacagt ggaagcgcct gtagaaccgc aatcgacaca actggctgtg tctgacgctg
    18421 ccgctgttac gcttcctgca acggtttccg cacctcccgt aatacccgca gtcgtcaaat
    18481 ccgcattcag cactgcacaa atagatcaat gggtcgcacc cgtcgcgctg tatcccgacg
    18541 ccctactttc gcaggtgctg atggcatcaa cctatccgac aaacgttgct caagcagtgc
    18601 aatggtcgca cgataatcca cttaaacaag gcgatgctgc tattcaggcg gtatctgacc
    18661 agccgtggga cgccagcgtt aaatcactgg tggcctttcc acaattgatg gcattgatgg
    18721 gcgaaaaccc gcaatgggtg caaaacctgg gcgatgcttt tctggcgcag ccgcaggacg
    18781 tgatggactc ggtacagcga ttgcggcaac tggcgcaaca aaccggctcg ctgaagtcat
    18841 caaccgaaca gaaagttatt accacaacga agaaagctgt accggtaaaa cagacagtca
    18901 cggcacccgt cataccatcc aataccgttt taactgccaa ccccgtcatt acagagccgg
    18961 caacaaccgt catttccatt gagcccgcca atcctgatgt ggtctatatt cccaactaca
    19021 acccaaccgt ggtttacggg aactgggcca atactgcgta tccgccggtt tatctgccac
    19081 caccagccgg agaaccgttt gttgacagct ttgtgcgcgg attcggctat agcatgggcg
    19141 ttgctaccac gtacgcacta ttcagcagca tcgactgaga tgacgacgat catgaccatc
    19201 atcatcatga cgatgatgat tatcatcacc acgatggcgg tcatcgtgac agtaatggct
    19261 ggcaacacaa cggcgacaac atcaatatcg acgtcaacaa tttcaaccgt atcaccggtg
    19321 agcatcttac tgataagaat atggcatggc ggcacaatcc aaactaccgt aatggtgtgc
    19381 cctatcatga tcaggatatg gcaaagcggt ttcatcaaac cgatgtcaac ggcggaatga
    19441 gtgccacgca gctacctgct ccaacacgcg acagccagcg tcaggcggca gcaagtcagt
    19501 ttcagcaacg aacacacgcc gcccccgtca ttacacgaga tacccaacgt caggcagcgg
    19561 cacagcggtt taatgaagct gaacactatg ggagctatga cgacttccgc gacttcagcc
    19621 gtcgccaacc actgacccag cagcaaaagg acgccgctcg tcagcgttat cagtcggcct
    19681 ctcctgagca gcgccaggca gtccgcgaga aaatgcagac taacccgcag atccagcagc
    19741 gaagggaggc ggcgcgccag cgtattcagt ccgcctcgcc tgagcagcgc caggcggttc
    19801 gcgagaaaat gcagactaac ccgcagaacc agcagcgaag agaggcagcg cgtgagcgta
    19861 ttcagtctgc aactcccgaa cagcgccagg tgtttaagga aaaagtacag cagcgcccac
    19921 tgaaccaaca gcaacgtgat aacgcccgcc agcgtataca atcggcatca cctgaacaac
    19981 gtcaggtttt tcgggagaaa gttcaggaga gccgcccaca acgtctaaac gacagtaacc
    20041 atactgtcag gctgaataac gagcaacggt cagcagtacg cgaacgtctc tctgaacgtg
    20101 gagcaagaag actggaaagg taaattgcag acgtaaaaaa agcggcgtgg ttagccgctt
    20161 ttttaattgc cggatgttcc ggcaaacgaa aaattacttc ttcttcgctt tcgggttcgg
    20221 caggtcggta atgctacctt cgaacacttc tgccgccagg cccacagact cgtgcagagt
    20281 cgggtgcgcg tggatggtca gtgcgatgtc ttcagcatca caacccattt cgattgccag
    20341 gccgatttca cccagcagct cgccgccgtt ggtaccaaca atcgcaccac cgatcacacg
    20401 gtgagattct ttgtcgaaaa tcagcttggt cataccgtct gcgcagtcag aagcgatagc
    20461 acgaccagaa gcagcccacg ggaaggtggc ggtttcatag ctgatgcctt tctctttcgc
    20521 ttctttctca gtcagaccta cccatgcaac ttctggttcg gtataggcga tggacgggat
    20581 aactttcgga tcgaagtagt gtttcttacc ggcgataact tcagcggcaa cgtgaccttc
    20641 gtgaacacct ttgtgtgcca gcatcggctg accgacgata tcgccgatag caaagatgtg
    20701 cggtacgttg gtacgcagct gtttgtcaac gcggatgaaa ccacggtcgt caacttccac
    20761 gccagctttg cctgcgtcga ggtttttacc gttcggcaca cgaccaatcg ctaccagcac
    20821 ggcgtcgtaa cgctgcggtt cagctggtgc ttttttgcct tccatcgtca cataaatacc
    20881 gtcttctttc gcttcaacgg cggtaacttt ggtttccagc atcaggttga atttcttgct
    20941 gatacgcttg gtgaagactt taacgatgtc tttgtcagct gccgggataa cctggtcgaa
    21001 catttcaacc acgtcaatct gtgaacccag cgcgtggtaa acggtgccca tttccagacc
    21061 gatgataccg ccacccatta ccagcaggcg ttctggtact tctttcagtt ccagcgcgtc
    21121 agtggagtcc cagatacgcg gatcttcatg cggaataaac ggcagttgga tcgggcgaga
    21181 acccgctgca atgatcgcat tgtcgaagtt gatcacggtt ttgccgttct caccttcaac
    21241 ttccagggtg ttagccccgg tgaatttacc cagaccgttg accactttga ctttgcggcc
    21301 tttcgccata ccagccagac caccggtcag ctgattgatc actttctctt tccaggtacg
    21361 aatcttgtca atatcggttt tcggttcgcc gaagacgata ccgtgttcag ccagcgcttt
    21421 ggcttcttcg ataacttttg ctacgtgcag cagtgcttta gaagggatac agccgacgtt
    21481 caggcaaaca ccgccaaggg tgttgtaacg ttctacgatt acggtttcca gacctaaatc
    21541 agcgcaacgg aaggcagcgg agtaacctgc ggggcctgcc ccaagtacca cgacctgagt
    21601 tttgatttca gtactcatca tgacctctat atatttatct ccggcggtca tacccgtcgt
    21661 ctttcaggcc gcagcggcgt tagcttcacg ccctcacccc agtcacttac ttatgtaagc
    21721 tcctggggat tctggcgctt gctaccttgc tgcacccaga aatccatagg gtatgtgaag
    21781 taatacccta accaccaccg ggtcgttcta tccgtcggta ttttacaaaa ttgttaacaa
    21841 tttttaaaca acaaacggca accgatttgt ctattcgcta ataacctcaa tacattcatg
    21901 agattaccag aaaaaagccg gccgttgggc cggctctttt acttacatca ccagacggcg
    21961 aatgtcagac agcgtgttgt taatgatggt aatgaaacgg gcaccatcag caccgtcgat
    22021 cacgcggtgg tcgaaggaga gagaaatcgg cagcatcaga cgcggcacga actctttacc
    22081 attccacacc ggctccatcg cggacttgga aacgccgagg atagccactt ccggcgcgtt
    22141 cacaatcggc gcgaagtggg tagtacccag gccgccgatg ctggagatgg tgaagcaacc
    22201 gccctgcatt tcgcccgcag tcagcttacc gtcacgcgct ttcttagaaa tagtcatcag
    22261 ctcgcgagac agctcgatga tgcctttctt gttgacgtct ttgaataccg gaacaaccag
    22321 accgttcggg gtatccaccg ccacaccgat gttgatgtat ttcttcaggg tcagacgctg
    22381 accgtcttcc gacagcgaac tattgaagcg aggcatctgc tcaagagctg cagcaacggc
    22441 tttcatgatg aagacaaccg gggtgatctt cacatccagc ttacgtttcg ccgcttcttc
    22501 gttctgctgt ttacggaacg cttccagctc ggtgatatcg gttttgtcga agtgggtaac
    22561 atgcgggatc atcacccagt tacggctcag gttagcacca gaaattttct ggatacggcc
    22621 cagttccact tcttcgattt cgccaaactt gctgaagtcc accttcggcc acggcagcat
    22681 gccagggata ccaccgccag tcgccgccgg agctgcttct gcacgtttga tagcttcttt
    22741 cacgtaagcc tgaacgtctt cgcgcaggat acgaccttta cggccagtgc ccttcacttt
    22801 cgccaggtta acgccaaact cgcgtgccag acggcggatc agcggggtcg cgtgaacgta
    22861 agcgtcgttt tcagcaaatt cagatttgcc ttccgctttc gcagccggag cttcagcttt
    22921 tgctgccgga gccggcgctg ccgcttcctg tttcgcagga gctgccgcag gcgctgcgcc
    22981 ttcaacttcg aagatcataa tcagcgaacc agttttcact ttatcgccaa cgttgacttt
    23041 cagttccttc acgacgcctg caaacggagc cggaacttcc atagaagctt tatcgccttc
    23101 tacggtaatc agtgactgtt cagcggcaac tttgtcgccc actttcacca tcacttcggt
    23161 cacttcaact tcgtcaccgc cgatatccgg aacgttaact tctttcacgc cagctgctgg
    23221 tgcaggggtc gctgccggag tcgcttcctg tttagccgcc ggagctgccg cgcctgcttc
    23281 acccgcgact tcgaagacca taatcagcga gccggtagac actttgtcac ccacgttcac
    23341 tttgatctct ttcacggtac ctgcaaacgg agccggaact tccatagaag ccttgtcgcc
    23401 ttctacggtg atcagcgact gttcagcttc aactttatcg ccaactttca ccaggatttc
    23461 ggtcacttca acttcgtcgc tgccgatatc cggaacgtta acgtcttttg ccgccgcagc
    23521 cgcgggtgct gctgccggag ctgcttcttt cttctcttct gcctgagcag gtgcagcgtc
    23581 tgctgcaccg tcggcggaat cgaaaatcat aatcagtgcg ccggtctggg ttttatcgcc
    23641 aacagagact ttgatctctt taacgatacc cgcctgcgga gatggaactt ccatagaggc
    23701 tttgtcgcct tctacggtga tcagcgactg ttcggcttca actttgtcgc ccactttgac
    23761 caggatctcg gtgatttcaa cttcatcagc cccgatgtcc ggtactttga tttcgatagc
    23821 cattattctt ttacctctta cgccagacgc gggttaactt tatctgcatc gatgttgaat
    23881 ttggcgattg cgtcagcaac cactttctta tcgatttcgc cacgtttagc cagttcgccc
    23941 agcgccgcaa ccacgacata agaagcatca acttcgaagt ggtgacgcag gttctcacgg
    24001 ctgtcggaac gaccgaagcc atcagtaccc agtacgcgat agtcgtcagc cggtacgtaa
    24061 gtacggacct gctcagcgaa cagtttcata tagtcggtag atgccactgc cggcgcgtcg
    24121 ttcatcacct gagcgatata cggaacgcgc ggagtttcca gcggatgcag catgttccag
    24181 cgttcacaat cctgaccatc acgcgccagc tcggtgaagg aggtcacgct ataaacgtca
    24241 gaacctacgc cgtaatcttt cgccaggatc tctgccgctt cacggacgtg acgcaggatg
    24301 gaaccggagc ccagcagctg aactttacct ttgctacctt caatggtttc gagtttgtag
    24361 atacctttac ggataccttc ctcagcacct tccggcattg ccggcatgtg gtagttttcg
    24421 ttcagcgtag tgatgtagta gtaaacgttc tcttgttttt caccgtacat acgctccaga
    24481 ccgtcatgca taatgacagc aacttcgtaa gcgtaagccg ggtcgtaaga gatacagttc
    24541 gggatagtca gcgactgaat gtggctgtga ccatcttcgt gctgcagacc ttcaccgttc
    24601 agggtggtac gaccggaagt accgccgatc aggaagccac gcgcttgctg gtctccagcc
    24661 gcccagcaca gatcgccgat acgctggaag ccgaacatcg agtaatagat gtagaatggg
    24721 atcatcggca gattgttcgt gctgtaagag gtcgccgctg ccagccagga acaccctgcg
    24781 cccagctcgt tgatcccttc ctgcagaatc tgacctttct cgtcttcttt atagtaagca
    24841 acctgctcgc ggtcctgcgg ggtgtactgc tgaccgttcg ggctgtaaat accaatctga
    24901 cggaacagac cttccatacc gaaagtacgc gcttcgtcgg cgatgatcgg taccagacga
    24961 tctttgatcg acttgttctt cagcatcacg ttcagagcac gaacgaaagc gatagtggta
    25021 gagagctctt tgctctgctc ttccagcagc gcgccgaagt cttgcaggct cggcagctca
    25081 agcttctcgg tgaagttcgg ctgacggctt ggcagataac cgtgcagttt ctgacgctga
    25141 gcgtgcagat aggtatgctc ttcagaacct tccgggaagg tgatgtacgg cagtttttcg
    25201 atatctgcat cagacaccgg cacattgaaa cggtcgcgga tgtgacgcac gccgtccatg
    25261 ttcattttct taacctggtg cgcgatgttt ttaccttcag ccgcgtcgcc catgccgtaa
    25321 cctttaatgg tatgagcaag gattactgtc gctttgcctt tggtttcctg cgctttcttg
    25381 aatgcagcgt agattttctt cggatcgtga ccaccacggt tcagtgccca gatctgctcg
    25441 tcagtccagt ctgcaaccag tgctgcggtt tcaggatatt taccgaagaa gtgttcacga
    25501 acgtacgcac catctttcga tttgaaggtc tggtagtcgc cgtcaacggt ttcgttcatc
    25561 agctggatca gtttaccgct ggtatcttta cgcagcagtt catcccaacg gctaccccac
    25621 atcactttga tcacgttcca gccagcacct tcgaagatgc cttccagttc gttgatgatc
    25681 ttgccgttac cggtgaccgg gccgtcaaga cgctgcaggt tacagttgat aacgaaaacc
    25741 aggttatcca gtttttcacg ggtagcgatg gtgatcgcac ctttggattc cggttcgtcc
    25801 atttcaccgt cgccgaggaa cgcgtaaacg gtctgtttag aggtatcttt caggccacgg
    25861 tgttccagat atttcaggaa tttagcctgg taaatagcac caatcggacc cagacccata
    25921 gatacggtcg ggaactgcca gaattccggc atcagtttcg ggtgcggata ggaagagagg
    25981 ccattgccgt gaacttcctg acggaagtta tccagctgct cctgagtcag acgaccttcc
    26041 aggaaagcac gggcgtaaac gcccggggag atgtggccct ggaagtaaac caggtcgccg
    26101 ccatcctgct cgttgcgtgc acggaagaag tggttaaagc acacatcata aatggttgcg
    26161 gaagactgga aggacgccat gtggccgccc agttcgaggt cttttttcga cgcacgcagc
    26221 accgtcatga tggcgttcca gcggatagct gaacgaatac ggcgttccag ttccagatta
    26281 cccggatact ccggttgttc ttcaacgggg atggtgttga tgtagttgct gatacctgtg
    26341 cctgcggcta cgtttacacc gcctttgcgg gcttcagcaa gcagttggtc gatcagatac
    26401 tgagcacgct caacaccttc ttcacggatg accgattcga tcgcctggag ccagtcgcga
    26461 gtttcgatcg gatccacgtc atttgggaaa cgttctgaca tgggttattc cttatctatc
    26521 taataacgtt gagttttctg gaacctgtcc cattgaactc tcgccagaaa gctcaataag
    26581 acaggttcta cgtttagtta ccgcgctttt atatgcgctt gatttacaac atcttctgga
    26641 taatttttac cagaaaaatc actaattctt tcgttgctcc agacgacgca gagaacgctc
    26701 acggcggctc tcttcacggc ttctgtcgag caaaatttct tcgataaagg ccagatggcg
    26761 atgcgatgct tcgcgcgctt cttccggctt accgaccata atcgcttcaa atatgcgggt
    26821 gcggtgacta ctcaccagcg gcagcatctc gcgacgcgaa tagagcaatt cgaagttctg
    26881 gcggacattc tgggccaaca tcggctccat acaccttagc agatgaagca gaaccacatt
    26941 gtgggccgct tcggtgacgg caatctgata ctggagtacg gcgtttgatt ccgcgtccag
    27001 atcgccagac tgctgcgcca gctctatggc gtggtggagt tcacggatgc gttccttgtc
    27061 ttcatcggta ctacgcagcg cggcgtaata agcggcgata ccttccaggg cgtgtcgtgt
    27121 ttcgagcaag tcatactgtg attcaggatg gtcggagagc agctccacca gcggatcgct
    27181 gaagctttgc cataggctgc tctggacaaa agtgccgcca ccctggcgac gaagcaacaa
    27241 gcccttcgct tcgagacgtt gaatcgcctc acgcaaggag ggacgggaga cgtcgaactg
    27301 ttttgccagt tcgcgttccg gtgggagttt ttcgcccggg cggagagtgc cttcgaggat
    27361 caaaaactcc agttgctgct caatcacatc ggagagtttt ggttggcgga ttttgctgta
    27421 ggccatgagt tcctgtctta agccacttgc cgaagtcaat tggtcttacc aatttcatgt
    27481 ctgtgacgct aaagtaacaa agtattcacc ttatgtccat acaggttttg attgaaatca
    27541 tgaaactgtg cacattttaa caacttgaca tatataacgt ttcaaagttg taactatgca
    27601 caaatgtaga ctttacgtag gaaagggatt ttttaacgat tattaaacta taaaaatccg
    27661 aattgaaccg attcacttac caattttttg atttttaagt caataaaaac gaatttaaat
    27721 tcactctgca tattgagagg ggttgaggct gagctttaca aacggtttct ttttaagcaa
    27781 ctcatcttca accatgcata aagcgggtgc attcgctgcc gcataccatt attcttgatc
    27841 tgacggaagt ctttttgtaa caattcaaac ctccttgatg taaacaaatc aatacaacaa
    27901 acggaattgc aaacttacac acgcatcact gcgtagatca aaaaaacaat caccgcacga
    27961 ggtttcatga tggaaggtca acagcacggc gagcagctaa agcgcggcct taaaaaccgc
    28021 catattcagc ttatcgcgct gggtggcgcg atagggacag ggctattcct aggtagcgcc
    28081 tccgtaatac agtccgcagg gccagggatt atcctgggtt acgccattgc tggttttatc
    28141 gcctttctga tcatgcgtca gctgggtgaa atggtggtcg aagaacctgt cgcaggctcc
    28201 tttagccact ttgcttataa atactggggc agttttgccg gtttcgcctc tggctggaac
    28261 tactgggtac tgtacgtttt agttgccatg gcagagctga ctgccgtggg taaatacatt
    28321 cagttctggt atccggaaat cccaacctgg gtttctgccg ccgtattctt tgtggtgatt
    28381 aacgccatca acctgaccaa cgttaaagtg tttggcgaga tggagttctg gtttgccatt
    28441 atcaaagtta ttgcggtagt agcgatgatc atcttcggcg gctggctgct gttcagtggc
    28501 aacggcggcc cgcaggcgag cgttagcaac ctgtgggatc aaggtggttt cctgccgcac
    28561 ggcttcaccg gactggtgat gatgatggcg attatcatgt tctcgttcgg tggtctggaa
    28621 ctggtgggga tcaccgcagc agaagctgat aacccggagc aaagtatccc gaaagcaact
    28681 aaccaggtta tctaccgcat cctgattttc tatattggtt cgttagccgt tctgctctca
    28741 ctgatgccgt ggacccgcgt taccgccgat accagtccgt ttgtgctgat cttccacgag
    28801 ttaggcgata cctttgtggc gaatgcgctg aacatcgtgg tactgactgc ggcgctctcc
    28861 gtgtacaaca gctgcgtata ttgcaacagc cgtatgctgt ttggtctggc acaacagggg
    28921 aacgcgccaa aagcgctggc gtctgtcgat aaacgcggcg taccggttaa caccattctg
    28981 gtgtctgcgc tggttacagc attgtgcgta ttgattaact atcttgctcc ggaatccgca
    29041 tttggcctgt taatggcact ggtggtatcc gcactggtga tcaactgggc gatgatcagt
    29101 ctggcgcata tgaagttccg tcgcgccaag caggaacaag gcgtggtaac tcgcttccct
    29161 gctctgcttt atccgctggg taactggatc tgcctgctgt ttatggcggc ggtactggtg
    29221 attatgctga tgaccccagg aatggcgatt tcggtatacc tgatcccggt atggctgatc
    29281 gtgttaggta tcggctatct gtttaaagag aaaaccgcca aagccgtaaa agcgcattaa
    29341 tctctctacg ccctcacccg tacagggtga gggcaataat ctctacacca acgccccgta
    29401 aatcgtcagt agcgcaatca ccaccacgac cacgaacgag gttttcttcg ccattgaaac
    29461 cgctgccttc ggcgtctcca ccttatcgac atgcggttca cgcgccagag agaactgcgc
    29521 cagacgcgtt aatacctgat attgcgaagt atggaaatca cccagcgaag caaaccaggc
    29581 cggtaacgct ttctcaccat gaccgatcaa ggcatatacc acacccgcaa gacgaaccgg
    29641 cacccaatcc agtacatgaa gcactgcatc aatgccggac tgtaaacgat gatgcggcgt
    29701 ctggtatcgc gccagccagt attgccatgc acgcaagaac gcatacccca tcagcgtaac
    29761 gggtccccag gttcccccca caatcagcca gaacagcggt gcaagataaa aacgaaagtt
    29821 aatccacagc aatgcatttt gcagctcacg caaatactca cgttcgtcgc agcctgccgg
    29881 gacgccgtga atcatggtga gttcgccagc catcgtggca cgggcatggc tatcattacg
    29941 tgaagcagct gtcagataag catgataatg aagacgaact ttacctgcgc caatacacag
    30001 caaaccaatc agcagccaca ccagcagcgt gggaacgttg aacaatactc cctgcaatgc
    30061 gcgtaacagt aaaaaagtca cacccatcgc aataatggtc atgcctaacg tgcgcccgag
    30121 agaaaaatgt ttcacccgcc gaaagaacgc ttcaagacga tgatcaagct gccagtgctc
    30181 gcccaactta aacaggcgct cgaaaattaa caccagtaag gttgtaaata gcgtcatgtt
    30241 gtctccttgc tgaccagcgc acgaaaccgt gcccaatcaa atgcaggacc gggatcggtt
    30301 ttccgatccg gcgcaatatc acaatggccc gtcatgttat tagcgatatc gggatagcga
    30361 tctatcagtg cccgcgtaac cgccgcaagc tgttgatact gcgcatcggt atacgccaga
    30421 gtatcggtgc cttcaagctc aatcccaata gaaaaatcat tgcagcgttc acgcccctga
    30481 tactgagaga ctcccgcatg ccaggcacgt ttatcgaaag gaacatactg gactatttca
    30541 ccatcacggc gaatcaaaca gtgagcggag acgcgcaaat gggcgatctc agcaaagaaa
    30601 ggatgtgcct gcggatcaat agttccagtg aataatgcgt cgatccacgg accgccaaac
    30661 tcgcctggcg gcaggctaat attgtgcacc accagcaggg tgggtgtttc gtcatccagg
    30721 cggcaatcgt aatgtggtga gggaacgcgg cgcgcgccaa ccagccaccc ctgttctaac
    30781 aacatgcagg atctccttat atgtggtgct aatacccggt tcagagtagc atgtttctac
    30841 cttatgattc gttagctatc tggagtttta acatgccgcc tcgccgctac aaccctgaca
    30901 cccgacgtga cgagctgctg gaacgcatta atctcgatat ccccggcgcg gtggcccagg
    30961 cgctgcggga agatttaggc ggaacagtcg atgccaacaa tgacattacg gcaaaacttt
    31021 taccggaaaa ttctcgctct catgccacgg tgatcacccg cgagaatggc gtattttgtg
    31081 gcaaacgctg ggttgaagag gtgtttattc aactggcagg cgacgatgtc accataatct
    31141 ggcatgtgga tgacggcgat gtcatcaacg ccaatcaacc cttgttcgaa cttgaaggcc
    31201 catcccgcgt gctgttaacg ggcgaacgca ctgcgcttaa ttttgtgcaa accctttcag
    31261 gagttgccag taaggtacgc cactatgtcg aattactgga aggcaccaac acgcagttgt
    31321 tggatacgcg caaaacctta cctggcctgc gttcagctct gaaatacgcg gtactttgcg
    31381 gcggcggagc gaatcaccgt ctggggcttt ctgatgcctt cctgatcaaa gaaaaccata
    31441 ttattgtctc cggctcagtg cgccaggcgg tcgaaaaagc gtcctggctg catccggatg
    31501 cgccagtaga agtcgaagta gagaatctgg aagaacttga tgaggccctg aaagccggag
    31561 ccgatatcat catgctggat aacttcgaaa cagaacagat gcgcgaagcc gtcaaacgca
    31621 ccaacggcaa ggcgctactg gaagtgtctg gcaacgtcac tgacaaaaca ctgcgcgaat
    31681 ttgccgaaac gggcgtggac tttatctccg tcggtgcgct aactaaacac gtacaagcac
    31741 tcgacctttc aatgcgtttt cgctaattgc tgacagattt atccgctcca tcgcgggcgg
    31801 ataatctccc cttccccaac tttcttcgta acgcctcgca aatttcttgt ctttcgctgc
    31861 aacgaaataa aaaacctcgg aatctgcttt cccgtttcgt tttttgcccc tcgcctgtgc
    31921 tgatggcgtt tgccaaagta gcaccaacca aatcaaggag cgaaacagat ggacaagcaa
    31981 cgcggtttta cacttatcga actgatggtg gttattggca tcattgccat tttaagctcc
    32041 attggtattc ccgcttatca aaactacctg cgcaaagccg cactcaccga catgctacaa
    32101 acctttgtgc cttaccgtac cgccgtagag ttgtgcgcgc tggaacatgg tggattagat
    32161 acctgcgacg gtggcagcaa tggcattccc tcgcctacca ccacccgcta tgtttcagcc
    32221 atgagtgtgg caaagggcgt ggtgtcgctg accgggcaag aaagtctcaa tgggctaagc
    32281 gtcgtcatga cgccaggttg ggataacgca aacggcgtca ccggctgggc gcgcaactgc
    32341 aatattcaaa gtgacagcgc attgcagcaa gcctgcgaag atgtcttccg ctttgatgac
    32401 gccaactaag gagcggcaat gaatattcca cagctcacgg ccctgtgcct gcgttatcag
    32461 ggagtcttgc tggatgccag cgaagaagtg gttcatgttg cggtggtcga tgccccctca
    32521 catgagttgc tggacgcatt gcatttcgct accaccaaac gtattgagat cacctgctgg
    32581 acgcgccaac aaatggaagg tcacgccagt cgcacacaac agacattacc cgtagctgtt
    32641 caggagaagc atcagcccaa agcagagttg ctgactcgaa cgttacaatc tgcgctggaa
    32701 caacgcgcgt ctgatattca tatcgaacca gcggacaatg cctaccgcat ccgcttgcgt
    32761 atcgacggcg tattgcatcc tttaccggat gtttcaccgg atgccggagt cgcattaaca
    32821 gccagattaa aagtgctggg aaacctggat attgcggaac atcgcctgcc gcaggacggg
    32881 caattcactg tcgaactggc aggaaacacc gtctcatttc gtattgcgac cttaccatgt
    32941 cggggtggtg aaaaggtggt attaaggttg ttacagcagg tgagccaggc actggatgtt
    33001 aacacgctgg gaatgcagcc gttacaactg gcggactttg ctcatgcctt gcaacaacca
    33061 cagggactgg tgctggtaac tggccctaca ggcagtggca aaacggtcac ctggtgttgt
    33121 ccacgctaca caccaattcc accactgaaa cgctggtacg tttacagcaa atgggggtcg
    33181 cccgctggat gctatcatcg gcgcttacgc tggtaatagc ccagcgtctg gtacgtaaac
    33241 tttgcccaca ttgtcgccga cagcaagggg agcccatcca tattccagac actgtatggc
    33301 cgtcgccgct gccccactgg caggcacccg gttgtgtaca ttgctaccac ggtttttatg
    33361 gtcgtaccgc cttgtttgaa gtcctgccca ttacaccagc catacgccag cttatttccg
    33421 ctaatactga agttgaatcg ctggaaacgc acgcccgaca ggcgggtatg cgtacgcttt
    33481 ttgaaaacgg ctgcctggcc gtggagcaag gcttaaccac ctttgaagag ttaatccgcg
    33541 tactggggat gccgcatggc aagtaagcaa ctctggcgct ggcatggcat aaccggcgac
    33601 ggcaatgcgc aagatgggat gctatgggca gagagccgta ctttactgct tatggcacta
    33661 cagcaacaga tggttacccc actaagcctg aagcgaatcg ccatcaattc tgcgcagtgg
    33721 cgaggagata aaagcgcgga agtcattcat caactggcga cgctacttaa agccgggtta
    33781 acgctttctg aagggctggc tctgctggcg gaacagcatc ccagtaagca atggcaagcg
    33841 ttgctgcaat cgctggcgca cgatctcgaa cagggcattg ctttttccaa tgccttatta
    33901 ccctggtcag aggcatttcc gccgctctat caggcgatga tccgcacggg tgaactgacc
    33961 ggtaagctgg atgaatgctg ctttgaactg gcgcgtcagc aaaaatccca gcgtcagttg
    34021 accgacaaag tgaaatcagc gttgcgttat cccatcatca ttttagcgat ggcaatcatg
    34081 gtggttgtgg caatgctgca ttttgttctg ccggagtttg ccgctatcta taagaccttc
    34141 aacaccccac taccggcact aacgcagggg atcatgacgc tggcagactt tagtggcgaa
    34201 tggggctggc tgctggtgtt gttcggcttt ctgctggcga tagccaataa gttgctgatg
    34261 cgccgcccga cctggcttat cgcgcggcag aaattgctgt tacgcatccc gattatgggt
    34321 tcactgatga ggggacaaaa actcacgcag atctttacga ttctggcgct gacacaaagt
    34381 gcaggcatta cttttttaca gggcgtagag agcgtcagag aaacaatgcg ctgcccgtac
    34441 tgggtgcaac ttctgacaca aatccagcac gatatcagta acggtcatcc catctggctg
    34501 gcgctaaaaa atgccggtga gtttagcccg ctctgtttgc aattagtgag aacaggagag
    34561 gcatccggct cgctggacct catgttagac aacctcgccc atcatcatcg ggataacaca
    34621 atggcgctgg cggataacct cgcagcctta ctggaaccgg cgttgctgat cataacggga
    34681 ggaattatcg gtacgctggt ggtggcaatg tatctgccaa ttttccattt aggcgatgcg
    34741 atgagtggga tgggataacg ctggcgtgtt gctccacgcc agcgttggga gattacaggt
    34801 tgttgaagat gcggttttct tgttccagca cacgaataaa cgtggtgcgc ttggtcagct
    34861 ctttcaggcg tgaagcccca acgtatgtac aagctgaacg caggccgccc aaaatatcac
    34921 gcgcggtatt ttcaacctgg cctcgcagcg gcagcttaac ggttttacct tctgctgcgc
    34981 ggtattccgc aacgccgcca acgtgacgtt tcatcgcaga ctcggagctc atgccgtaga
    35041 acagcataaa tttctcgccg ttctcctcaa cgatgcgacc gccgctctct tcgtggcccg
    35101 ccagcatgcc gccaagcatg acgaaatcgg caccgccgcc aaaggctttc gccacatcgc
    35161 ccggcgtggt acagccaccg tcgctgacga tcattccgcc cagaccgtgc gccgcatcgg
    35221 cacattcgat taccgcagaa agctgcggat aaccgacgcc tgttttgacg cgagttgtac
    35281 aaacagaacc tgggccaatg ccaactttaa cgatatcagc accggagagg ataagctcct
    35341 cacacatttc accagtcact acgttgccag cacaaatggt tttggtcggc cacgcttcac
    35401 gcgctttcgc aacgaactgc acgaagtgtt cggaataacc attcgccaca tcaatacaaa
    35461 cgaagtttaa tgccgggttc aggtcgagaa tctgtttagt tttttcgaaa tccgcatcag
    35521 acgtgccggt agaaaccatc acatgtttca gcacatcagc ggaagaattg ttgataaacg
    35581 cttgccactc ttcgacagaa aagtgtttat gcacagcagt cagaatatca aaagaagcca
    35641 gcgcagaggc catagaaaat gtgcctacgg tgtccatatt tgcggcgata atcggcacgc
    35701 cggaccagct ctgacctgaa tgtttgaagg tgaattgacg ttccagttca acatcggaac
    35761 ggcttttaag agtggagcgt ttagggcgga tgagaacgtc tttaaaacct aacttcagat
    35821 cttcttcaat acgcatgtgc ggattcctgg ggttaatggc tataagggta agagcgcaac
    35881 tccagtgacg ctatcatacg cactaatcaa tgcggcgcaa gactgcgaaa ttgtcttttt
    35941 ttacgctaca atcccataaa tttacctggt aaatagtgag cgaacgtttc atcaatttca
    36001 ctttcgcgcg ctcattttta acgttttgat aatcatgatg aattcccggg aatataaatt
    36061 atggggtata tagttgcctt aacgggaggc attggcagtg gtaagagtac cgttgccaat
    36121 gcgtttgctg atctcggaat taacgtcatt gatgccgata ttattgcgcg tcaggtggtt
    36181 gaaccaggtg cacctgcgct acatgccatt gctgatcact ttggcgaaaa catgattgct
    36241 gctgatggaa cattgcagcg ccgggccttg cgcgagcgga tcttcgccaa cccggaagag
    36301 aaaaactggc ttaacgacct gctgcatccg ctgattcaac aagagacgca acaccagatc
    36361 cagcaagcaa cttcccccta tgtactgtgg gttgtgccat tgctggtaga aaactcactg
    36421 tataaaaaag cgaatcgagt gctggtggtg gatgtcagcc cagaaacgca acttaagcgc
    36481 accatgcagc gcgatgatgt aactcgcgaa catgtcgaac aaatccttgc tgctcaggca
    36541 acgcgcgaag cccgccttgc cgtggcagat gacgtcattg ataataacgg cgcaccggat
    36601 gctatcgcat cggatgttgc ccgcctgcac gcacactatt tgcagcttgc gtcgcagttt
    36661 gtctcacagg aaaaaccgta atgcagaccc aggtcctttt tgaacatcca ctaaatgaaa
    36721 aaatgcgtac atggctgcgc attgagtttt tgattcagca actcaccgtt aatttaccca
    36781 tcgttgacca cgctggcgcg ctgcatttct tccgtaatgt cagtgaatta ctggatgttt
    36841 tcgagcgcgg cgaagtccgc actgagctgt tgaaagaact taaccggcag caacgtaaac
    36901 tccagacctg gattggcgtg cctggcgtgg accagagccg tattgaagca ttaattcagc
    36961 agttaaaagc ggcggtgagc gtattaattt ccgcgccgcg tatcgggcaa tttctgcgtg
    37021 aagatcgttt gattgctctg gtgcgtcagc gactgagcat cccaggcggc tgttgcagct
    37081 ttgatttacc tacattgcac atttggctgc atctacccca ggcgcagcgc gacagccagg
    37141 tagaaacctg gattgccagc ctgaacccgc tcacccaggc acttaccatg gtgctggatt
    37201 taattcgcca gtcggccccc ttccgtaaac aaaccagcct gaatggtttt tatcaggata
    37261 acggtggcga tgccgacttg ctgcgcctga atctgtcgct cgattcacag ctttatccgc
    37321 aaatttccgg acataagagc cgttttgcca ttcgttttat gccgctggac actgaaaacg
    37381 gacaggtacc ggaacgtctg gatttcgaac tggcctgttg ctaaggagta aaaatgtcag
    37441 aaactattac ggtgaattgc ccaacctgcg ggaaaacggt ggtgtggggt gaaatcagcc
    37501 cgtttcggcc attttgctcc aaacgttgtc agctgatcga cctcggagaa tgggctgctg
    37561 aagaaaaacg aatccccagc agtggcgatc tttccgaaag cgatgactgg agcgaagagc
    37621 caaagcagtg acatttgtct gatgcctcac gtaggcctga taagacgcgg acagcgtcgc
    37681 atcaggcatc ttgtgcaaat gtcggatgcg gcgtgaacgc cttatccgac ctacagacgc
    37741 ttaagcttcg caattaccgg ttcattggct ggcggaaaat catcggcatt aagaccgacc
    37801 agcgacatcc actcaccggg ttgcccttct ttaccccacg gccccccttc ccaactttcg
    37861 accagccaga accacagagt tatatgcctg tccgggaatt catattccag tttttcaaat
    37921 agcgaaaaat gttgtggggt aatcccgacc tcttcctgaa gttcacgcac caccgcctgt
    37981 tccggcgttt cgcccatttc aattttaccg ccgggaaact ccagtttatt cgccatgtgc
    38041 gcatctgctg cgcgacgtgt tataaagatt tcattgttct cgttgcgaat aatacctacc
    38101 gcaatttgca gctttttcat tgtcttaaac ctataaaaaa ggcgcagaat cctgcgcctt
    38161 ttacttcaac tgttagcttt tattgcaggc gaccatggca ctgcttgtat tttttaccag
    38221 aaccgcacgg gcaaggatcg ttacgtccta ctttgcgttc accggtttgc gccgccagtg
    38281 cagctgcggc tgcagagtcg tcatcctgat ggctaagctg ctgcatttgt gctaaacgct
    38341 cggcttccat acgacgctgt tgttccagct cctcaacctc ttcaggcata cgtacctgaa
    38401 ctttgctcag cgtactgata acttcatatt tcaacgactc cagcatcgct gcaaacatgg
    38461 agaacgattc acgtttgtat tcctgcttcg gatctttctg tgcatagcca cgcaggtgga
    38521 taccctgacg cagatagtcc atcgctgcca ggtgctcttt ccacagggag tcaagcgttt
    38581 gcagcatgac gcctttctcg aagtgacgca tcatctcagc accaaccact tcttctttac
    38641 gctgatacac ttcgatggac tgcgccagaa tgcgctcacg cagcgtctct tcatgcagtt
    38701 ctggttcttt atccagccac tcggtaattg gtaaatcgag gtcgaaatcg ttcttcagac
    38761 gttcctgcag ccccggaata tcccacattt cttccagcga ctgtggcgga atgtaggcat
    38821 caatggtggc tttgaacaca tcttcacgaa tgctgttgat ggtttcgctc acatcgctga
    38881 catccagcag ttcgttacgc tgggagtaaa tggcgcgacg ctgatcgtta gccacgtcat
    38941 catattccag cagttgctta cgaatgtcga agttacgact ttcaacttta cgctgggcgt
    39001 tggcaatcgc tttagtcacc cacgggtgtt caatggcttc gcctggcttc atacccagtt
    39061 tacgcatcat gccggatact cggtcggaag caaaaatacg catcagcgca tcttccatcg
    39121 acaggtagaa acgggaagaa ccagcatccc cctgacgacc agaacgaccg cgcaactggt
    39181 tatcgatacg acgggattcg tgacgctcgg taccgatgat atgcaggcca cctgcttcca
    39241 gtaccgcatc gtgacgtacc tgccagtcgg ctttaatttt ttcaatttgc tctgcggtcg
    39301 gattttccag cgcggcaact tctgcctgcc agctacctcc gagcacaata tctgtaccac
    39361 gacccgccat gttggtcgcg atagtcaccg cagccggata acctgcctga gcaacaatcg
    39421 ccgcttcgtt ggcgtggaat ttggcgttca ggacattgtg cttaataccg gctttggtca
    39481 gttcgtttga caccagctcc gatttttcga tggagatagt acccaccagc accggctggc
    39541 ctttcgcagt acgttctttg atatcttcaa tgatcgcctg aattttttcc gcttcagtca
    39601 tgtagaccag gtccggcaga tctttacgaa tcattggacg gttggtcgga acaacgacgg
    39661 tatccagttt gtagatggag ctaaattcga aagcttcggt atcagcagta ccggtcatcc
    39721 ccgccagttt ttcatacaga cggaagtagt tctggaaggt gatcgaagcc agcgtttggt
    39781 tttcgttctg gatctgcaca ccttctttcg cttccacagc ctggtgcaga ccatcggacc
    39841 agcgtcggcc ctgcatggta cgaccggtgt gttcgtcaac gatgataact tcaccatctt
    39901 taacgatgta gtcgacgtca cgggtaaaca gcgcatgagc gcgcagcgcc gccgttacgt
    39961 ggtgcatcag catgatgttg gccggagagt acagagactc cccttcatcc atgatgccct
    40021 ctttcaccag cagttcttca atcagcacca gaccacgttc ggtcaggttc acctggcgag
    40081 atttttcgtc caccgagaag tggccttcgc cctggaaggt ttcggagtct tctttttcct
    40141 gacggatcag gtgcggaata attttattca cgcgtttata catttccgag ctgtcttctg
    40201 ccgggccgga aatgatcagc ggtgtacgcg cttcatcgat caggatggag tccacttcgt
    40261 ccaccagcgc atagtgcagt ttacgctgta cacgttcttc agggctgaac gccatgttgt
    40321 cgcgcaggta gtcaaagccg tattcgttgt tcgtaccgta agtgatgtca gctgcgtaag
    40381 cttcgcgctt tgccggtgct ggcatgcccg gcaggttgat accaacagtc aggccaagga
    40441 attcaaacag cggacggttg ttttcggcgt cacgttgcgc caggtagtcg ttgacggtaa
    40501 ctacgtgcac gcctttaccg gttagtgcgt tcaggtaagc aggcagcgtt gcggtcaggg
    40561 ttttaccttc accggtacgc atttcggcga tgcagcgttc gttaagaacc ataccgccga
    40621 gtaactgaac gtcgaagtga cgcataccaa agacgcgctt acttgcctca cgcaccacgg
    40681 cgaaagcttc cgggatcaga ttttccagca cttcgccttt ttccagacgc gcacgaaact
    40741 ctgcggtttt ccctttcagt tcttcatcgg agagtttttc catctccggt tccatggcat
    40801 tgatgatgtt gaccactttg cgcatccggc gcagggtgcg atcgttacga ctaccgaaaa
    40861 ctttagttaa caatttgatt agcataataa aatctcaaac gccccgcgtt acggagttaa
    40921 taaaatgaag taaaggttta ttgttgttag gtgaggcgtt gagggccagc acggatgcct
    40981 tgcgcctggc ttatccagac gggcgtgctg aattttgctt gtggggtaaa atgcgcataa
    41041 tcaatgcgat aacccttttc agacggcgtg ccttcctggg tcagcagcgc gctgagcgta
    41101 tccagtaatg caagatgttg cgcctgaaga ggcaaagatt cttcagcaac gggcagtgtt
    41161 tgcggtgcca ttgcgaaaga aagatgacgg attaccgtac gaatagcatg ttgatgccag
    41221 taatcaacgg aatagttcga attcgggcgg cgcgtgttcg cttccagcaa ggccaattga
    41281 ccaaagttaa ctttggctga aggctcgtgg ttgcgggttg tcgcttttgc gggcgcgttt
    41341 ggttcggcgg cgttgctgag cgcaggcaaa cctaaactcg ccgcgaccat ccctaataag
    41401 agatgcggcc agaagtagcg tttaccaaac tgtcgccagc gcgtcagtat tccactcacg
    41461 ttattgccat cccgatcgaa ttcaaacgta tttagcgcac aaatcttatc attaaagcct
    41521 gaagtccaca gcacgaatga agataagttg tgcgtaaaag tgcttaagaa aacagcgttc
    41581 gcaccgtttt ttatagctct tataaaattc aaaaaaaaac gactggttca cctggccgga
    41641 gagagtgcca gatttgccag tcgaatttta tacgacagta taaatgtcgt tatgccagta
    41701 cagctgaagg cgctttgaag gccaacggca gttctgcgtc gtcctggaag gtcacatatt
    41761 cccaggcttc ctgtttcgcc aggacagcct gcagcagttt gttattcagt gcatgaccgg
    41821 atttataagc ggtaaatgca ccaataatat tgtgaccaca catgaacaag tcaccgatcg
    41881 catcgagcat tttgtgacgc acaaattcgt cttcaaaacg caggccgtct tcgttcagta
    41941 cgcgataatc gtcaacaacg atggcacaat cgaagctgcc gcccaggcac aaaccacggg
    42001 actgcagata ttcgatatca cgcatgaaac cgaacgtacg cgcacggctg atctggcgca
    42061 taaacgcatc agcggagaag ttcatcgcat agcgctggtt gctggaatca atagccggat
    42121 ggttaaaatc gatggtgaaa tccagcgaaa aaccattgta cggcttaaat tcagcccact
    42181 tatcgccatc ttcgacacga acagtctctt tgatgcgaac aaattttttg gcgcagttca
    42241 actcgtcgat accggcgtca agcagcaggt atacaaacgg agcggcgctg ccgtccatga
    42301 tcgggatttc cggcgcgtta acttcgataa caatgttatc gatgcccagg cccgcgagag
    42361 cagcattgag gtgctctacg gttgaaatcc gtacatcatg ctcgttgacc agacacgtac
    42421 agagcatggt atcacgcaca gatttggcat cggccgggaa atctaccggt ggattcaagt
    42481 cggtgcgacg atagatgacc ccggtgttgg ccggcgcagg gcgtaacgtc agggtgactt
    42541 tcttgccggt atgtaaaccg acacccgtcg cctgaacgat acgtttaagt gtcctttgtt
    42601 tgatcatcgt attatctcgc caaattacct atccaaccga agtgtactat acattcggcg
    42661 ggccagttta gcacaaagag cctcgaaacc caaattccag tcaattctta atcagcttgc
    42721 ttacgtagga atgctgggat atccagataa tccggctctt tcgcagtttg cggcgcattg
    42781 tcattcacta ctttagcaac cggcttctgc tcctgggtca gcggagccat cccatgctgc
    42841 tggtagcgat ccatcactgg ctgctgaacc tgcttattgg tcaccagagt gatttcagga
    42901 cgtttgtcca tgccgatacc tgtcgcaaca acggttacgc gcagctcgtc attcatatcc
    42961 gggtcaagag aagtaccgat aaccacagtc gcgttgtcgg aagcaaatgc acggatggtg
    43021 ttacctaccg tttcgaactc atccagacgc aggtcgaagc ccgccgtgat gttaaccagc
    43081 acgccgcgcg cgccagacag gtcgatatct tccagcagcg gagaagagat agccatttca
    43141 gcagcttctt ccgcacggtc ttcaccgctc gccacgccag aacccatcat tgcgtagccc
    43201 atttcagaca ttacggtgcg tacgtctgca aagtccacgt tcatcaaacc cggacgagta
    43261 atcagttcgg cgataccttg cacagcgcct ttcagtacat cgttcgctgc gccaaacgca
    43321 tccagcaggg agataccgcg gcccagaact ttcagcagtt tgtcgttcgg gatagtgatc
    43381 agagagtcca catgcttgga cagttcagtg atcccctgct cggcgaatgc catacgcttc
    43441 ttgccttcaa agttgaaagg cttagtgacg acagcaacgg tcaggatacc caaatctttt
    43501 gccacttcag cgacgactgg tgctgcacct gtaccggtac caccacccat acccgcagca
    43561 ataaagacca tgtctgcacc ttccagcgcc gcacgcaatg catcgcgatc ctcatcagcc
    43621 gcattgcggc caacttctgg attagcgcca gcgcccagtc ctttggtgat accgctaccg
    43681 atttgaatcg tctgtccaac cgctgtttta cgcagcgctt gtgcatcggt atttaccgcg
    43741 aagaattcaa caccttcaat acgctcgcgc accatgtgtt caacagcatt accgccgccg
    43801 ccgccgacgc cgatgacttt aatcaccgcg tcattggtaa gttccattgg ttcaaacata
    43861 gtttctctcc gatttgtgcc tgtcgcctga gtccgtaatc atcgtcggcc tcataaaaat
    43921 taaaactctt ttcgcagcca actattgagt cgcttgatcc acgagccaac tgatgctgta
    43981 acacgttttt ctacttcagc ttcaccgtta agatgtgact ctttcccata gtgaagcaat
    44041 cccaccgccg tcgaataata cggctcctga gcataatccg ttaaaccggt aatgttcagc
    44101 ggcgcgccga tacgcacttg cgtatgaaac acgcgctgag cacaggctgc aagaccttcg
    44161 atctgcgctg cgccaccggt taatacaatg cctgccgcca ggtgatgttt aaccccttgt
    44221 tggcgaagct tttcctgcaa ctgcaatatc tcttcgttga ccaggttgag caactcggta
    44281 tagcgcggct cgatcacctc tgccagggtc tgacgttgca gactccgcgg cggacgaccg
    44341 cctacgctcg gcacttccac gctctcatct tttccaacga tggaacccag cgcacaaccg
    44401 tggcgaactt taatcgcttc ggcgtcgctt ggcggcgtgc caaaggcgta agcgatatca
    44461 ctggtcacga cattgccagc ataaggaatt acctttgtgt ggcgcaatgc cccaccggta
    44521 taaacggcga tatccattgt accaccaccg atatcgacga cgcagacacc cagttcacgt
    44581 tcatcttccg tcaataccga ataacttgat gccagtccgg caaatatcag ttggtcaact
    44641 ttcagcccac aacgttcaac cgctttgacg atgtttttcg ccatatcgtt gtgacatgtg
    44701 atcaggtgca cttttgcctg catccgcaca cccgaaagtc ctaccggatt cttgatccct
    44761 tcctgatagt caatcgcata ctcttgcggg atcacatgca gcacacgatg ctcatcgcgc
    44821 acacgcaccg atttcgcggt atggacgacg ttttccacat cttcttgcgt cacttcttct
    44881 tcagaaatag gcaccatacc aatttcattc tggcagctga tgtgcttacc agaaagcgcc
    44941 agatataccg aagagatctg acaatctgcc atcaattctg cctggtcaat ggcgcgttgt
    45001 acgcacttga ccacggattc gaggtcgttc accccgcctt tatccatacc acgtgacggg
    45061 cagctgccca cgccaatgat attgaccata ccgtcgggca gaacttcccc tactaaagcg
    45121 gcaaccttcg cggtaccaat ttccagtcct actaccagtt ttctgtccgt cgccttgatc
    45181 attgttgttc tgcctgtgcc tgattttgtt gctgagtaga ttcctctggc ggcaagggcg
    45241 cccagcctac tgccgctcca gagtcataac gcaaatcaac gtagctaatc cgtttgccat
    45301 cggtttgcgc ctgctgctgt aaaaccggat aaagttctac aaagcgaacc aaacgtttca
    45361 tcgtatcgcc ccggccaaga ttgagcttaa tatcgttatt cagcgtcaac tgccaggaac
    45421 gccgcgcggt catcgccgct tccttcagag taaatctgtc ctttgccagc atctgcccca
    45481 tttcgcgata gccctgcaac acttcattag cgctgccttc cgggccatac agcattggaa
    45541 gcacctgctt gctggtgcga tctggcggca cgctgaaggt gtttccttcc gcgtctacca
    45601 tgtgttgatc attccaccgc gcaatcggca catattcaac cagatgaatc ttcaattcat
    45661 caggccactg ctttctgacg ctcacctgct taatccacgg caggcgttgt tctatttgcg
    45721 tctggatgat gttgacatcc tgggtcataa aggtacccgg ctcacccaat gccaggatcg
    45781 actgccggat atcgtcatta cgcgtgtaat ggcgttcacc ggtcaacacc agctttgaga
    45841 gcggcaggcg ttgcgcatct tccatccagc tcaacacgac ccagccgctc accaacactg
    45901 tcgttaaaac ggtcagcagg aaaaggatcc ccgccagacg cgttccatta ttgcggcgag
    45961 aagaaacctc ttcttcgctg tttcgcgtgt tcagagcagc ctgcgacata ttagtccgcc
    46021 agttccagaa ttcgtactac caactgcgag aagctcatac ctgcctgacg tgccgccatc
    46081 ggcaccaggc tgtggctggt catacccggt gaggtattgg cttccagcag ataaaactgt
    46141 ccatcactgt ccagcataac gtcaatacgt ccccatcctt tgcaacctaa cgtcgtccat
    46201 gctttcagca ctaatgcctg caaattggcc tcttgtgacg cttccagacc tgcggggcag
    46261 aaatactgtg tctcatcaga gagatacttc gcctcataat catagaaggt tccggacggt
    46321 tgaatacgta ttgacggtaa aatttcttca ccgagtatcg caaccgtgaa ctccggccca
    46381 cttagccatt tttcaatcaa tacttcttca tcgtgctgaa atgccaatct taatgcatct
    46441 tgtagagcat tttctgctac tacttttgac attcccacac tggaaccttc gcggctcggc
    46501 ttaacgataa ccggcaaacc cagagcagaa atttctgcta actgcttatc gctcaggcct
    46561 ttttcaaact ctgcgcgggt taacgctacc cacggcgcga ccggtaaacc ggcaccttgc
    46621 cacagaagtt tgctgcgtag tttatctatt gaaagcgcag atgccatcac tccgctgccg
    46681 gtataaggca agcccatcag ctcgagcatg ccctgcagcg taccatcttc accgccgcga
    46741 ccgtgtagcg cgataaacac tttctgaaaa cccatcgact tcagttgcgt cacgtcgact
    46801 tctttcgggt cgacaggata cgcatcaata ccgccttcac gcagcccggc taacaccgct
    46861 gcgccagaat tcagagaaac ttcccgctca gcggaagtcc cgcccaacag gaccgcgatt
    46921 ttatcagtca tgttgttctt cctccggagt ttgcggcttc agtttgattt cagctaaaga
    46981 acgggcaatt tttccgatat taccagcccc ctgaacgaga atcaggtcgt taccggttaa
    47041 taccggtgcc agcatctcgg ctacctgcgc agggtccggc accagaatgg gatcaatttt
    47101 cccacgtcca cgaattgtgc gacacagcga acggctgtcc gctcccggaa ttggcgcttc
    47161 gccagccgga tacacttcca gcatcaacag ggtatcaacc tgcgtcagca cattggcgaa
    47221 atcatcatac aggtcgcgcg tacgggtaaa acggtgcggc tgaaacagca ttaccaggtt
    47281 tttatccggc cagcctgcgc gcgccgcttt aatggtggcg tcaacttccg tcgggtggtg
    47341 gccgtagtca tcgaccagca ttgccgtacc gcttttacca ttcactggct ccagcgggaa
    47401 ttcaccgagg aaatcaaaac ggcgaccagt cccctggaag ctctccagcg cacgcaaaat
    47461 agcctcgtcg tcaattccct cttccgtagc aaccgcaacc gcagctgcgg cgttcagcgc
    47521 gttatgacgg cctggcgcat tcagggtgac gcacatcggc tctttgtcct gacgcagcag
    47581 cgtaaagtgc ccctgcgggc caatctgctg ataatcttct acacgcacgt cggcatcttc
    47641 gctgaagccg taagttgtgg tctgacgtcc cacacgcggt aacaattcgc ggatcaccgg
    47701 atcatcaaca cacatcaccg cacgaccgta aaaaggcagg ttgtgcagaa aattaataaa
    47761 ggtctgtttt aaattctcaa agtcgccctg gtaggtatcc atgtggtcgg cttcgatatt
    47821 ggtgacaatc gccaccatcg gttgcagatg caggaacgat gcatcactct catctgcttc
    47881 ggcaatcagg taccgaccat gccccaaacg cgcatgaacc cccgccgctt ttaccagccc
    47941 gccgttaacg aaggttgggt cgagccccgc ttctgcgtag atgctggaaa ccatcgcggt
    48001 agttgtcgtt ttgccgtgcg ttcctgcaat ggcgatgcca tgacgaaaac gcattaactc
    48061 agccagcatt tcggcacgac ggatcaccgg aatacgcgct tcatgagctg cgacaatttc
    48121 cgggttgtcg gcagaaatgg cgctggaaac aacgaccacg ctggcatcac gtacgttttc
    48181 cgggcgatgg ttgaaataaa tcgtcgcacc cagattcagt aactgctgcg tgaccggatt
    48241 tggcgctaaa tcggaaccac tgatctgata accttcattg gccagaactt cggcaatacc
    48301 gcccataccg gcaccaccaa tgccgacaaa atgtatgtgc cgaacgcgac gcatttcggg
    48361 cacgatggaa cgcagttttg ccaattgttg tgtattcatt ctttacgcca ttaacttctt
    48421 aaattcatac gatgcaaaag gcatcgctac aattacgccc gggcagcccg gctcacttca
    48481 tttgccactc gctcggtggc atccggaatg gatgcagcgc gggcgcgttc tgccatggtt
    48541 aataaggttt ctcgcgacca cccggccagg gtgttggcga cagcatccac gctaagctgt
    48601 ggctgctcga taattttggc tgcgcccgct ttttccagcg gtagcgcatt ccagtattgc
    48661 tggcggtctt tatgttgaaa cggcacaaac aacgccggta gtcctgccgc ggcgatttca
    48721 ctcacagtta aagcaccgga gcggcaaacg acgacatccg cccacgcata cgccgccgcc
    48781 atatcatcaa taaattcagt cactttatgc tgcggttgcc ccgcttcggc atacgcctgt
    48841 tcaacggatt gttgcgaacc tttgccgctc tgatgccaga tagtgactga atcacccagt
    48901 ttcgcagcaa cctgcggcat tgtctggtta agaatgcgtg cgccctggga accacccact
    48961 accaacacac gaaccggacc ttcacgtcca gccaaacgtt gctgcggcaa cggcagcgcc
    49021 agcacatcgg tacgcaccgg gttacccacc acttccgcat tagggaaagc gcctggaaac
    49081 gcctgcatca ctttggtagc aatcttcgcc agccatttat tggttaagcc cgcaataccg
    49141 ttttgttcat gaagtacaac cggaatgcct aacgaccacg cggccagacc acctggacct
    49201 gatacatagc cgcccatacc gagtaccacg tcaggtttgt acgctttcat aatcgcccgc
    49261 gcctgacgcc aggcgttgaa gatacgcagc ggcgcagcta tcagtgcttt tatacctttt
    49321 ccacgcaaac cagagatacg aatgaaatca atttcgatgc catgttttgg cactaagtcc
    49381 gcttccatac ggtcggcagt ccccagccag cgaacttgcc aaccctgagc cattagatag
    49441 tgcgcaaccg ccagtcccgg gaatacatgt ccaccggttc cgcctgccat caccattaat
    49501 cgctttcctt gaccactcat cgtgaacctc gtacaaacgc ctgcgctttc tccagacgcg
    49561 tttcataatc aatacgcaac agcatcatga tggctgtcga cataatcagt aagctcgaac
    49621 caccgtaact gattagcggc aatgtcagac ctttggtcgg taacatcccc gccgccgcgc
    49681 ctacgttaac cagcgcctgg aagctaaacc agatgccaat agaacaggcg agaaaaccgg
    49741 aaaaacggtg gtcaatttct aatgctttgc ggccaatcga catcgcacga aaagcgacga
    49801 agaataccat taaaagtgct agtaccacac cgacataacc taattcttcg ccgataatgg
    49861 cgaaaataaa gtcagtgtgc gcttccggca gatactccag tttttgtacc gaattaccta
    49921 aaccttgccc ccaaagttcg ccgcgaccaa acgccatcag cgattgcgtt aactgatagc
    49981 cgctgccaaa gggatcttcc cacgggttcc agaatgcggt aacacggcgg atacggtacg
    50041 gttcggcgag gatcagcaac acaaccgctg aaatgcccat accgataatg gcaatgaact
    50101 gccacaattt cgctcccgcc aggaacaaca tcgccagcgt agtcacaaac aacaccacca
    50161 ccgtaccaag gtctggctgt gccagcagta acactgccaa caccagaatc acgcccatcg
    50221 gtttcaggaa gccgcgcagg ttattacgta cttcgtcgcc tttacgcacc agatagttgg
    50281 cgatatagca aaacagcgac agttttgtca gctccgcagg ctggatacgc agcaaaccga
    50341 gatcgatcca acgcgatgcc cctttaacag cgctacccac taccagcacg atcatcagca
    50401 ggatgataga tccgagcagc atcgtggcac tgtagcgttg ccagaactcc atcggcagac
    50461 gcagcgtaat gatcgccaga ataaacgcca aaatcagata gacaccatca cgcttcgcga
    50521 agaagaacgg atcgttggtt aagcgttgcc ctatgggcat tgacgccgag gtcaccatga
    50581 taaagccaat cgccgcgagg ccgaaggtca gccacagtaa ggtgcgatcg tacatgatca
    50641 ggctgtcggt atctttttcc cgcgagccca tcacccagcc ctttagcgcc gtggagatcc
    50701 agaccaggat actgaatcct ggcaggcgcg gcattttcag gcgagggaga gataaacgca
    50761 tcaacctaac tccttcgcca gacgggcaaa ctcattgcct cgttgttcaa agttcttgaa
    50821 ctgatcaagg ctggcacagg ccggggagag cagaaccata tcgcccggct gaacacgcgg
    50881 agccagcaag cgcatagcct gttccatagt ttcggtttgt tctgccactt ccgggcgtag
    50941 cgccgccagc tgcgcgccgt cacgaccgaa acaatacaga cgtacgttat cgccattcag
    51001 gtaacgcacc agtgggctaa agtccgccga tttaccatcg ccgcccagca acaaatgcag
    51061 cgtgccgtct acgtgcaggc cattcagcgc cgcttccgta ctgccgacgt tggtcgcttt
    51121 tgaatcgtta atccagcgca caccgttatg ctccagcacc acttcaaagc gatgcggcag
    51181 accggtgaag gtggttaacg ctttcaggct gctggcgcgc ggtaaccctg cggcatctgc
    51241 cagcgccagc gccaccagcg cattggtgta gttatgctgc ccggaaagtt tcatctcttt
    51301 cacattcagc actttctcgc ctttaacccg cagccaggtt tcgccctgct gatgattcag
    51361 gtgatagtca cccatgttga cgccaaagct gacgcagcgt tcatccgcac cgcgaatcgg
    51421 cattgttaag gcatcatcag cattaaccac gcaaactttc gcgttttcgt aaatgcgcag
    51481 ttttgctgca cgatactgtt gtaaaccaaa cgggtagcga tccatatgat cttcagtcac
    51541 gttcagaatg gtcgctgcta ccgcctgtaa gctggaggtg gtttccagct ggaagctcga
    51601 cagttccagc acgtacagtt cacactcatc atccagtagc atcaacgcag gcaggccaat
    51661 attgccaccc acaccaacgt taacccccgc cgctttcgcc atttcaccca ctagcgtggt
    51721 gaccgtgctt ttgccgttag aaccggtaat cgccacaatc ggtgcttgtg cttcgcgaca
    51781 aaacagctcg atatcgccaa cgatttcgat tccggcatca gcggcagcgc ttaaggatgg
    51841 atgcgccagt gcaataccgg gactggcgac aatcagatct gccgccatca gccattcatc
    51901 attcagactg cccgtgtggc gttctacggc ttcgggtaat ttatccaggc caggcggtgt
    51961 catacgcgta tccataacgc gcggcgtcac accgcgagcg aggaaaaagt ccacgcagga
    52021 aagcccggtg aggcccaggc cgataatgac aacattttta ccctggtaat cagccatgat
    52081 taacgtacct tcagcgttgc cagaccaatc aggaccagca tcagcgaaat aatccagaaa
    52141 cgcacaatga cgcgcggttc cggccagcct ttcagttcat agtggtgatg aatcggtgcc
    52201 atgcggaaaa tacgttgtcc gcgcagttta aaggagccga cctgcaggat gacagaaagc
    52261 gtttctacca cgaacacgcc ccccataatc accagcagga attcctgacg tagcagtacg
    52321 gcgataatgc ctaacgcacc acctaacgcc agcgaaccga catcgcccat aaagacctgc
    52381 gccggatagg tgttaaacca caggaagccc agtcctgccc cgactatcgc ggtacagaca
    52441 ataaccagtt ccccggcgtg tcgcagatac ggtatgtgta aatagctggc aaagttcatg
    52501 ttgccggtcg cccacgccac cagcgcaaaa ccaccggcga caaatacggt cggcataatt
    52561 gccagaccgt caagaccatc ggtcaggttt accgcgttgc cagtacccac aatgacgaag
    52621 taagccagca gaatgtagaa cagccccagt tgcggcatca catctttaaa gaatgggacc
    52681 accagctgcg ttgcgggcgt gtctttgccg gcaaggtaca aagcgaaagc aacacccagc
    52741 gcaatgaccg acatccagaa atatttccaa cgagcgatca accctttggt gtctttacgc
    52801 accactttgc gataatcatc aacaaagcca ataacaccgt aacctaccag caccaccaac
    52861 acgcaccaga cgtacggatt ggacgggtaa gcccacagca gcacggagat cacaatcgct
    52921 gtcaggatca taatcccgcc catggtcggc gtaccgcgct tgctgaagtg tgattcagga
    52981 ccgtcgttac gcactacctg accaaaggaa agtttttgca aatgagcaat catgcgcggg
    53041 cccatccaca atgagatgaa cagcgcggtc agcaggctga cgatggcgcg aaacgtcaga
    53101 taggaaaaga cgttaaagcc ggaataatat ttgaccaaat gttcggccag ccaaactaac
    53161 atgtcccatt ctcctgtaaa gcgcgtacta cctcttccat ggcagcacta cgtgaaccct
    53221 taactaaaat cgtaattacc tgttgctcag caatcagtga tttaagacgc gtaattaacg
    53281 cagttttatc agcaaaatgt tcgccaacgc cgctggcggt gctgatagca tggctttgtt
    53341 tacccatgct taacacgcgg tcaataccag cagcttttgc cgcctcaccc acctgtacat
    53401 ggcaggcttc gctttcagcg cccagttccg ccatatcgcc caccaccagc acgcggtagc
    53461 ccggcatttc agccagtacc tgaacagctg cggtcattga accgacattg gcgttgtagg
    53521 agtcgtcgag cagcaactgg ttttctgcca gttggatggg gaacagacgg ccaggaacag
    53581 ctttcagatt tgccagcccc gctttgatag catcaagcgt tgcgcccacg gacatggaga
    53641 gcgcagcggc tgccagcgca ttcgcaatat tgtgacgccc cggcaacggc agcagaacat
    53701 cgacgctacc ggttggggtt tgtagggtaa attccatacc gtgcgaggtc acatggatat
    53761 tggtggcggt gaaatcgctg ttggcggcat tgggtgagaa acgccacact ttgcgtgagc
    53821 caattacgct ctgccagttc agccagtcgt tgttgtcggc gttcataatg gcgataccgt
    53881 tttccggcag gccgctaaag atttcacctt tcgctttcgc gacacccgca agcgagccaa
    53941 aaccttccag atgcgccgct gccaggttgt tgaccagcgc cgcttccggg cgagtcagac
    54001 tcacagtcca ggcaatctcg ccctgatggt tcgcgccaag ttcaataact gcgtaatcgt
    54061 attccggcgt taagcgcaac agcgtcatcg gtacaccgat gtcgttgttg agattgcctg
    54121 ccgtataaag cgtgttgccg cactggctta aaatcgccgc cgtcatctct ttaacggagg
    54181 ttttgccgga ggaccccgtc agagcaacca cgagcgccgg aacttgctgg cgaacccatg
    54241 cagccagttc accaaacgcc agacgcgtat ccttgacgat taactgcggc aggtcgatgt
    54301 ccagcggacg gctaaccagt agtgcgcctg cgccgccagc tttcgcctgg tcggcaaaat
    54361 cgtgggcatc aaaacgttca cctttcaggg caacaaacag gcagcccggc gtcagttttc
    54421 gggtatcagt ggttacagca tcaagggtga tatctgcacc ttgcagttca ccgttgagaa
    54481 tatcggtaag ttggctaagg gttacgctaa tcatgcaatc ccccccagca gacgcgccac
    54541 cgtgacgcga tcggagtagt ccagacgctg attgccaaca atctggtaat cctcatggcc
    54601 tttgcccgcg accagtacca catcattctc tttagcctgc ataacggcgc aagtcaccgc
    54661 ttcagcacgg ccttccatca ctttggcatg tccggcatct aacattcccg ccagaatatc
    54721 gttgatgatg gcacgcggtt cttcggtacg cgggttatcg tccgtcacca ccgccacgtc
    54781 agcaaactct tcggcaattg cgcccatcag tggacgctta cctttatcgc gatcgccacc
    54841 acagccaaag acgcaccaca gcttgcccgc acagtgcagg cgcgccgcct gtaaggcttt
    54901 ttccagtgca tccggcgtat gcgcgtaatc caccaccacc gtcggtttgc ctggcgcagt
    54961 gaacacttcc atacgtccgc aaaccggttg cagacgcgcg gcggttttca gcagatcagc
    55021 cagtggatag cccagcgcca gcagtgttgc cagcgcgagc aacaggttgc tgacgttaaa
    55081 agcgcccatc agatggcttt caatttcgcc atcgccccag cttgaactaa agcgaatggt
    55141 cgcgccgctg tcgtgatagt tcacttcggt cgctttcaac cagcgtccgt gacagttcgg
    55201 attgatatga tcttccattg ataccgcaac cgcgtccggc agttttgcca gccagcggcg
    55261 gcccacttca tcgtcggcgt taataatcgc ctgaccgcaa tgatgttcag aataaagcag
    55321 ccatttcgca gcttcgtagt gttccatatc accatgataa tcaaggtgat cgcggcttaa
    55381 gttggtaaag accgacgccg gaaatttcaa tgccgccaca cggtgctgta ccagcccgtg
    55441 ggaggaaact tccattgcgc aaaaagtcgc gccctgatcc accagccccg ccagctcatg
    55501 ctgaacatca actgccgaac cggtagtatt ttctgtcggg atcactttcc ccagcagacc
    55561 gttaccaacg gtgcccatta ccgcgctggt ttcgccaagc agttggctcc actgcgccag
    55621 aagctgggtc gtcgtggttt tgccgttggt gcccgttacg cctacgagac gtaaattgtc
    55681 agagggttca tggtaaaagc ggcccgccag tgcagataaa cgctcgttga gctggctgag
    55741 atagatgacc ggtacgccgt gcatttcacg gatttcaccg tcggtcgcct catctttcgc
    55801 ctctgcaata atggcagcca caccttgcgc tatcgcctgc gggatatatc gacgcccgtc
    55861 cgcctgatga cctactacag ctacaaagag atcgcccgcc gcagccacac ggctgtcgag
    55921 tgtcatctct cgcagtgctc gcgaaggtgc gtctggcacc cacggagcaa gaaggtcgcg
    55981 caaattacga tctgccacct gtcccctcgc cttgattaat cacaaattca tttttatcgc
    56041 ccgttgtcag cgcatccggc tcgatgttca tggtacgcaa tacgccgccc atgatggcac
    56101 caaagaccgg cgcggaaacg gcgccgccgt agtatttacc cgcctgcgga tcgttgataa
    56161 caacaaccag cgcgaagcgc ggctgactcg caggcgcaac gcctgcggta taagcaatat
    56221 atttattgat gtagcgaccg tccggcccga cctttttcgc ggtaccggtt ttaatggcga
    56281 tacgatagcc tttaatcgcc gccttcacgc cgccgccgcc tggtagcgcc acgctttcca
    56341 tcatatgcac cacggtgcga acaatggatt ccgggaagac acgttcaccg ggaaccgggg
    56401 ggtcaacttt ggtaatcgac agtgggcgat aaatgccgta gctgccgata gttgcgtaga
    56461 ctcgcgctaa ctgtaacggt gttaccatta gcccgtagcc gaaagagaag gtggccctct
    56521 ctatgtcaga ccaccgttgt ttttgaggat ataagccact gcgttctccg accaacccca
    56581 aattggtcgc ttttcccagt ccaaaacgtg agtaagtatc tactaacact gaggacggca
    56641 tcgttaacgc cagcttggaa acaccgacgt tactcgactt ctgtaatacc ccggtcaggg
    56701 ttaattcgct gtagcgtgcc acgtctttga tttcgtggcc gttaattcga taaggaatgg
    56761 tattgagtac cgagttttcc cgcaccacgc cacgttgcaa cgcggtcatc accaccatcg
    56821 gtttaaccgt tgagcccggt tcaaacacgt cggtgatggt acggttacgc atcgcctctt
    56881 tcggcgtgcc gctcagattg ttagggttgt atgacgggct gttagccatc gccagcactt
    56941 cacctgtgtt gacatccacc agcacggcgc taccagattc agccttgtta aaggccaccg
    57001 cgttgttcag ttcgcgataa accagcgcct gcaggcgttc atcaatactc agcgccaggt
    57061 tgtgcgctgc ctggctgtca gtagaagaaa tatcttcaat tacgcgacca tagcggtctt
    57121 tacgcacaat gcgctcaccc ggctgcccgg taagccattt atcgaaactc ttctcaacgc
    57181 cctcaatccc ttgactatcg acgttagtaa agccgatgag gtgagcagtc acttcgccgg
    57241 acggatagta acggcgagac tcttcacgca gatgaatccc cggcagtttc agttttttga
    57301 tgtagtccgc catgtcagag tttacctgac gcgccagata aataaagcgc cctttcgggt
    57361 tggcgttaat gcgggcagaa agctgatcca gcggaatatt gagcgcgtta gccagcgcct
    57421 tccagcggtc accgacgctg ataccgccag cgtcatgcac ttctttcggg tcagcccaaa
    57481 tcgcttttac cggcacgctc accgctaacg ggcgaccaga acggtcagta atcatgccgc
    57541 gggaggtgga aacttgctga acgcgaagag aacgcatgtc gccctctttc accagcatat
    57601 ccggggagat aacttgtaac cacgctacgc gtccgagcag aaaagccagc gccaggagaa
    57661 tacagccgca taacaacgca aaacgccaac tgataaagtt ggcatgttct tcctgacgtt
    57721 ttggtttctg cgttttcgcc gctgctttca tgcgtcgcgt ttatccttat ttttgcacta
    57781 cgatattttc ttgtgacgga tcaacatgct gcatttgcag cttttccgtg gcgatccttt
    57841 ccacccggct atggtcgccg agcgcattct cttcaaggat caggttgcgc cattcaatgt
    57901 ctaaagcatc tcgctccagc accagttgtt cgcgctgagc ggtcagtaaa cgggtatggt
    57961 gcgccgtggt taccacggtc accgccgtca aaataatgca aatgaacagg cagagtggca
    58021 gcttcccaaa tcgcaaaaga tcgtcaccga taacaccagg caatgcatgg cgctcgtggc
    58081 ttcccatcga tcctttaact ttgcttagag cttctgtcac tctgctgatc atgcgttcgt
    58141 cctctctgca atacgcagaa ctgaactacg ggcacgaggg ttctcagcca cctcttcttc
    58201 gcccggcatt aacttgccta gtgctcgcag ctgacggcca cccagttttt tgagctgctc
    58261 ttcagtcatc ggtaaccctg ccggaacttg cggaccgcgg ctgttttcac gcataaaacg
    58321 tttcacaata cggtcttcca gcgagtggaa gctgatgatc gaaagccgcc cacccggggc
    58381 cagcacgttg agcgagcttt ttagcgcctg ctctatctcc tccagttcac tgtttaccca
    58441 aatgcgcacc gcctggaagg tacgggtcgc gggatgttta aacttatctt tcaccggcgt
    58501 tgcagcagcc acgacttccg ccagttcttt ggtgcgggtc atcggctgtt cgcggttacg
    58561 ctcgacaatg gcgcgggcaa tgcgtttggc aaaatgctct tcaccatagg ttttcaatac
    58621 ccaggcgata tcggcttctt ctgcggtttg tagccattca gcggctgact gcccacgggt
    58681 tgggtccata cgcatgtcca gcggaccatc gcgcataaag gaaaagccgc gttcagcatc
    58741 atcaagttgc ggtgaagaga cgccaagatc gaggagaatg ccgtcgatct tgccaataag
    58801 atcgcgctcg gcaacgtatt cgcccagcgc ggagaaaggt ccgtggatga tggagaagcg
    58861 cggatcatca atagtcttcg caacggcgat agcctgcggg tcgcgatcga tcgccagcaa
    58921 acgcccctct tcgccaagct gcgagaggat cagacgtgag tgaccaccgc gaccaaaagt
    58981 cccatcaatg tagatgccat caggacggat attgaggcca ttaacggctt catccagcag
    59041 caccgtagta tgtttatagt tttccatcat tttatagaga caagtcctgc agtcgctccg
    59101 ataagtctcc ggtagccaac tgctctgcgt cgatatcttc cttgacctgt tgatgccagg
    59161 ttgtttcatc ccacagctca aacttgttga actgtccaac cagcatcact tcttttgtca
    59221 gcccggcatg ttgccgcagt actggcgcga ttaacaatcg acctgcgcca tccatctgac
    59281 attcgctggc atgacctaac agtaggcgct gcacacggcg ctcaaccggg ttcatgctca
    59341 acagacgcga taatttttgc tcgataattt cccattcagg cagggggtaa agcagcaggc
    59401 acgggtgatg aatgtcaatg gtgcaaacca tttgaccggc agcgttctca agcagctgtt
    59461 cccgataacg ggtaggcact gataagcgcc ctttgctgtc gagattgact aacgttgctc
    59521 cccggaacat gccagcctca ccccttattc ccactttgcc ccacaatttc ccactgaaag
    59581 gagtttacgg agcggaggaa aagcttgtca agccagtcac aaggcatacc gaggtaaaaa
    59641 acccgatgtt tacggctaat atcagcgttg tttattttac gtccaacgtg cgaagcaaat
    59701 taacgttatg aatagctgta agaaaaaatc cctcatcgtc atcactgttt taaaatgact
    59761 cactattttc cacagcaaat ataaagtgtc agtttgcgac gcgagcggca ttttaggaca
    59821 tatcttcccc ggttaacagt ccttgttacg tctgtgtggc gcttgatgcc agcgggtttg
    59881 cgcaaaagca aagaaaagtg tttgttaaat cggcaattca tcgccgcatg taacaaaata
    59941 atgcaaaaac gcgctgtcga tatcacttaa ttattgtgaa tttcagctgg tgagttaatt
    60001 aacgggcggg tttaaaacca tttatcaata atagtcagga atagtcttat ttactttaag
    60061 gatattgat
//
LOCUS       DAJTRC010000013        58069 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.013, whole genome shotgun sequence.
ACCESSION   DAJTRC010000013 DAJTRC010000000
VERSION     DAJTRC010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58069)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58069)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58069
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.013"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..1100)
                     /locus_tag="OO357_000878"
     CDS             complement(<1..1100)
                     /locus_tag="OO357_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose phosphorylase"
                     /protein_id="HCR5885916.1"
                     /translation="MKQKITDYMDEIYGGTFTATHLQKLVTRLESAKRLITQRRKKHW
                     DESDVVLITYADQFHSNDLKPLPIFNQFYHQWLQSIFSHVHLLPFYPWSSDDGFSVID
                     YHQVASEAGEWQDIQQLGECSHLMFDFVCNHMSAKSEWFKNYLQQLPGFEDFFIAVDP
                     KTDLSAVTLPRALPLLTPFQMDDNSTRHLWTTFSDDQIDLNYRSPEVLLAMVDVLLCY
                     LEKGAEYVRLDAVGFMWKEPGTSCIHLEKTHLIIKLLRSIIDDVAPGTVIITETNVPH
                     KDNIAYFGNGDDEAHMVYQFSLPPLVLHAVQKQNVEALCQWAQSLSLPSSKTTWFNFL
                     ASHDGIGLNPLRGLLPESEILALVEALQQEGA"
     gene            complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="OO357_000879"
     CDS             complement(1312..1659)
                     /gene="pspE"
                     /locus_tag="OO357_000879"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase PspE"
                     /protein_id="HCR5885917.1"
                     /translation="MAIFIHRRTLTCLKKGLLALALVFSLPVFAAEHWIDVRVPEQYQ
                     QEHVQGAINIPLKEVKERIATAVPDKNDTVKVYCNAGRQSGQAKELLSEMGYTHVENA
                     GGLKDIAMPKVKG"
     gene            complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="OO357_000880"
     CDS             complement(1702..1923)
                     /gene="pspD"
                     /locus_tag="OO357_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707216.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspD"
                     /protein_id="HCR5885918.1"
                     /translation="MNTRWQQAGQKVKLGFKLAGKLVLLTALRYGPAGVAGWAIKSVA
                     RRPLKMLLAVALEPLLSRAANKLAQRYKR"
     gene            complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="OO357_000881"
     CDS             complement(1932..2291)
                     /gene="pspC"
                     /locus_tag="OO357_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspC"
                     /protein_id="HCR5885919.1"
                     /translation="MAGINLNKKLWRIPQQGMVRGVCAGIANYFDVPVKLVRILVVLS
                     IFFGLALFTLVAYIILSFALDPMPDNMAFGEQLPSSSELLDEVDRELAASETRLREME
                     RYVTSDTFTLRSRFRQL"
     gene            complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="OO357_000882"
     CDS             complement(2291..2515)
                     /gene="pspB"
                     /locus_tag="OO357_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response membrane protein PspB"
                     /protein_id="HCR5885920.1"
                     /translation="MSALFLAIPLTIFVLFVLPIWLWLHYSNRSGRSELSQSEQQRLA
                     QLVDEAKRMRERIQALESILDAEHPNWRDR"
     gene            complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="OO357_000883"
     CDS             complement(2569..3237)
                     /gene="pspA"
                     /locus_tag="OO357_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein PspA"
                     /protein_id="HCR5885921.1"
                     /translation="MGIFSRFADIVNANINALLEKAEDPQKLVRLMIQEMEDTLVEVR
                     STSARALAEKKQLTRRIEQASAREVEWQEKAELALLKEREDLARAALIEKQKLTDLIK
                     SLEHEVTLVDDTLARMKKEIGELENKLSETRARQQALMLRHQAANSSRDVRRQLDSGK
                     LDEAMARFESFERRIDQMEAEAESHSFGKQKSLDDQFAELKADDAISEQLAQLKAKMK
                     QDNQ"
     gene            3404..4381
                     /gene="pspF"
                     /locus_tag="OO357_000884"
     CDS             3404..4381
                     /gene="pspF"
                     /locus_tag="OO357_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage shock protein operon transcriptional
                     activator"
                     /protein_id="HCR5885922.1"
                     /translation="MAEYKDNLLGEANSFLEVLEQVSHLAPLDKPVLIIGERGTGKEL
                     IASRLHYLSSRWQGPFISLNCAALNENLLDSELFGHEAGAFTGAQKRHPGRFERADGG
                     TLFLDELATAPMMVQEKLLRVIEYGELERVGGSQPLQVNVRLVCATNADLPAMVNEGT
                     FRADLLDRLAFDVVQLPPLRERESDIMLMAEHFAIQMCREIKLPLFPGFTERARETLL
                     NYRWPGNIRELKNVVERSVYRHGTSDYPLDDIIIDPFKRRPPEEAIAVSENTSLPTLP
                     LDLREFQMQQEKELLQLSLQQGKYNQKRAAELLGLTYHQFRALLKKHQI"
     gene            complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="OO357_000885"
     CDS             complement(4501..5766)
                     /gene="puuE"
                     /locus_tag="OO357_000885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate transaminase"
                     /protein_id="HCR5885923.1"
                     /translation="MSNNEFHQRRLSATPRGVGVMCNFFAQSAENATLKDVEGNEYID
                     FAAGIAVLNTGHRHPDLVAAVEQQLQQFTHTAYQIVPYESYVTLAEKINTLAPVSGQA
                     KTAFFTTGAEAVENAVKIARAHTGRPGVIAFSGGFHGRTYMTMALTGKVAPYKIGFGP
                     FPGSVYHVPYPSDLHGISTQDSLDAIERLFKSDIEAKQVAAIIFEPVQGECGFNVAPK
                     ELVAAIRRLCDEHGIVMIADEVQSGFARTGKLFAMDHYADKPDLMTMAKSLAGGMPLS
                     GVVGNANIMDAPAPGGLGGTYAGNPLAVAAAHAVLNIIDKESLCERANQLGQRLKNTL
                     IDAKESVPAIAAVRGLGSMIAAEFNDPQTGEPSAAIAQKIQQRALAQGLLLLTCGAYG
                     NVIRFLYPLTIPDAQFDAAMKILQDALSD"
     gene            complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="OO357_000886"
     CDS             complement(5804..7084)
                     /gene="puuB"
                     /locus_tag="OO357_000886"
                     /EC_number="1.4.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylputrescine oxidase"
                     /protein_id="HCR5885924.1"
                     /translation="MTEHTSSYYAASANKYAPFDTLNESITCDVCVVGGGYTGLSSAL
                     HLAEAGFDVVVLEASRIGFGASGRNGGQLVNSYSRDIDVIEKSYGMDTARMLGSMMFE
                     GGEIIRERIKRYQIDCDYRPGGLFVAMNDKQLATLEEQKENWVRYGNKQLELLDVNAI
                     RREVASDRYTGALLDHSGGHIHPLNLAIGEADAIRLNGGRVYELSAVTQIQHTTPAVV
                     RTANGQVTAKYVIVAGNAYLGDKVEPELAKRSMPCGTQVITTERLSEDLARSLIPKNY
                     CVEDCNYLLDYYRLTADNRLLYGGGVVYGARNPDDVERLVVPKLLKTFPQLKGVKIDY
                     RWTGNFLLTLSHMPQFGRLDTNIYYMQGYSGHGVTCTHLAGRLIAELLRGDAERFDAF
                     ANLPHYPFPGGRTLRVPFTAMGAAYYSLRDRLGV"
     gene            complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="OO357_000887"
     CDS             complement(7086..8573)
                     /gene="puuC"
                     /locus_tag="OO357_000887"
                     /EC_number="1.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase PuuC"
                     /protein_id="HCR5885925.1"
                     /translation="MNFHHLAYWQDKALSLAIENRLFINGEYTAAAENETFETVDPVT
                     QAPLAKIARGKSVDIDRAVSAARGVFERGDWSLSSPAKRKAVLNKLADLMEAHAEELA
                     LLETLDTGKPIRHSLRDDIPGAARAIRWYAEAIDKVYGEVATTSSHELAMIVREPVGV
                     IAAIVPWNFPLLLTCWKLGPALAAGNSVILKPSEKSPLSAIRLAGLAKEAGLPDGVLN
                     VVTGFGHEAGQALSRHNDIDAIAFTGSTRTGKQLLKDAGDSNMKRVWLEAGGKSANIV
                     FADCPDLQKAASATAAGIFYNQGQVRIAGTRLLLEESIADEFLALLKQQAQNWQPGHP
                     LDPATTMGTLIDSAHADSVHTFIREGEGKGQLLLDGRNAGLAAAIGPTIFVDVDPNAS
                     LSREEIFGPVLVVTRFTSEDQALQLANDSQYGLGAAVWTRDLSRAHRMSRRLKAGSVF
                     VNNYNDGDMTVPFGGYKQSGNGRDKSLHALEKFTELKTIWISLEA"
     gene            complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="OO357_000888"
     CDS             complement(8723..9280)
                     /gene="puuR"
                     /locus_tag="OO357_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001464354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator PuuR"
                     /protein_id="HCR5885926.1"
                     /translation="MSDEGLAPGKRLSEIRQQQGLSQRRAAELSGLTHSAISTIEQDK
                     VSPAISTLQKLLKVYGLSLSEFFSEPEKPDEPQVVINQDDLIEMGSQGVSMKLVHNGN
                     PNRTLAMIFETYQPGTTTGERIKHQGEEIGTVLEGEIVLTINGQDYHLVAGQSYAINT
                     GIPHSFSNTSAGICRIISAHTPTTF"
     gene            complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="OO357_000889"
     CDS             complement(9307..10071)
                     /gene="puuD"
                     /locus_tag="OO357_000889"
                     /EC_number="3.5.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415814.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase"
                     /protein_id="HCR5885927.1"
                     /translation="MENIMNNPVIGVVMCRNRLKGHATQTLQEKYLNAIIHAGGLPIA
                     LPHALAEPSLLEQLLPKLDGIYLPSSPSNVQPHLYGENGDEPDADPGRDLLSMAIINA
                     ALERRIPIFAICRGLQELVVATGGSLHRKLCEQPELLEHREDPELPVEQQYAPSHEVQ
                     VEEGGLLSALLPECSNFWVNSLHGQGAKVVSPRLRVEARSPDGLVEAVSVINHPFALG
                     VQWHPEWNSSEYALSRILFEGFITAWQHHIAEKQRL"
     gene            10283..11701
                     /gene="puuA"
                     /locus_tag="OO357_000890"
     CDS             10283..11701
                     /gene="puuA"
                     /locus_tag="OO357_000890"
                     /EC_number="6.3.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-putrescine ligase"
                     /protein_id="HCR5885928.1"
                     /translation="METNIVEVENFVQQSEERRGSAFTQEVKRYLERYPNTQYVDVLL
                     TDLNGCFRGKRIPVSSLKKLEKGCYFPASVFAMDILGNVVEEAGLGQEMGEPDRTCVP
                     VLGSLTPSAADPEFIGQMLLTMVDEDGAPFDVEPRNVLNRLWQQLRQRGLFPVVAVEL
                     EFYLLDRQRDAEGDLQPPCAPGTDDRNTQSQVYSVDNLNHFADVLNDIDELAQLQLIP
                     ADGAVAEASPGQFEINLYHTDNVLEACDDALALKRLVRLMAEKHKMHATFMAKPYEEH
                     AGSGMHIHISMQNNRGENVLSDTEGEDSPLLKKMLAGMIDLMPSSMALLAPNVNSYRR
                     FQPGMYVPTQASWGHNNRTVALRIPCGDRHNHRVEYHVAGADANPYLVMAAIFAGILH
                     GLDNELPLQEEVEGNGLEQEGLPFPIRQSDALGEFIENDHLRRYLGERFCHVYHACKN
                     DELLQFERLITETEIEWMLKNA"
     gene            11850..12014
                     /locus_tag="OO357_000891"
     CDS             11850..12014
                     /locus_tag="OO357_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885929.1"
                     /translation="MPMIKSPHGEGGCVCAPPATDWTPPPLLPLLNRFDFRSTRPQTL
                     LRRGGSNYGY"
     gene            12004..13389
                     /gene="puuP"
                     /locus_tag="OO357_000892"
     CDS             12004..13389
                     /gene="puuP"
                     /locus_tag="OO357_000892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707205.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PuuP"
                     /protein_id="HCR5885930.1"
                     /translation="MAINSPLNIAAQPGKTRLRKSLKLWQVVMMGLAYLTPMTVFDTF
                     GIVSGISDGHVPASYLLALAGVLFTAISYGKLVRQFPEAGSAYTYAQKSINPHVGFMV
                     GWSSLLDYLFLPMINVLLAKIYLSALFPEVPPWVWVVTFVAILTAANLKSVNLVANFN
                     TLFVLVQISIMVVFIFLVVQGLHKGEGVGTVWSLQPFISENAHLIPIITGATIVCFSF
                     LGFDAVTTLSEETPDAARVIPKAIFLTAVYGGVIFIAASFFMQLFFPDISRFKDPDAA
                     LPEIALYVGGKLFQSIFLCTTFVNTLASGLASHASVSRLLYVMGRDNVFPERVFGYVH
                     PKWRTPALNVIMVGIVALSALFFDLVTATALINFGALVAFTFVNLSVFNHFWRRKGMN
                     KSWKDHFHYLLMPLVGALTVGVLWVNLESTSLTLGLVWASLGGAYLWYLIRRYRKVPL
                     YEGDRTPVSET"
     gene            13523..13768
                     /locus_tag="OO357_000893"
     CDS             13523..13768
                     /locus_tag="OO357_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmjA family protein"
                     /protein_id="HCR5885931.1"
                     /translation="MNHDIPLKYFDIADEYATECAEPVAEAERTPLAHYFQLLLTRLM
                     NNEEISEEAQHEMAAEAGINPVRIDEIAEFLNQWGNE"
     gene            14081..15724
                     /gene="sapA"
                     /locus_tag="OO357_000894"
     CDS             14081..15724
                     /gene="sapA"
                     /locus_tag="OO357_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein SapA"
                     /protein_id="HCR5885932.1"
                     /translation="MRQVLSSLLVIAGLVSGQAIAAPESPPHADIRDSGFVYCVSGQV
                     NTFNPSKASSGLIVDTLAAQFYDRLLDVDPYTYRLMPELAESWEVLDNGATYRFHLRR
                     DVPFQKTDWFTPTRKMNADDVVFTFQRIFDRNNPWHNVNGSNFPYFDSLQFADNVKSV
                     RKLDNHTVEFRLAQPDASFLWHLATHYASVMSAEYARKLEKEDRQEQLDRQPVGTGPY
                     QLSEYRAGQFIRLQRHDDFWRGKPLMPQVVVDLGSGGTGRLSKLLTGECDVLAWPAAS
                     QLSILRDDPRLRLTLRPGMNVAYLAFNTAKPPLNNPAVRHALALAINNQRLMQSIYYG
                     TAETAASILPRASWAYDNEAKITEYNPAKSREQLKALGLENLTLKLWVPTRSQAWNPS
                     PLKTAELIQADMAQVGVKVVIVPVEGRFQEARLMDMSHDLTLSGWATDSNDPDSFFRP
                     LLSCAAIHSQTNLAHWCNPKFDSVLRKALSSQQLAARIEAYDEAQSILAQELPILPLA
                     SSLRLQAYRYDIKGLVLSPFGNASFAGVYREKQDEVKKP"
     gene            15721..16686
                     /gene="sapB"
                     /locus_tag="OO357_000895"
     CDS             15721..16686
                     /gene="sapB"
                     /locus_tag="OO357_000895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapB"
                     /protein_id="HCR5885933.1"
                     /translation="MIIFTLRRILLLIVTLFLLTFVGFSLSYFTPHAPLQGASLWNAW
                     VFWFNGLIHWDFGVSSINGQPIAEQLKELFPATIELCILAFGFALIVGIPVGMIAGIT
                     RHKWQDNLINAIALLGFSIPVFWLALLLTLFCSLTLGWLPVSGRFDLLYEVKPITGFA
                     LIDAWLSDSPWRDEMIMSAIRHMILPVITLSVAPTTEVIRLMRISTIEVYDQNYVKAA
                     ATRGLSRFTILRRHVLHNALPPVIPRLGLQFSTMLTLAMITEMVFSWPGLGRWLINAI
                     RQQDYAAISAGVMVCGSLVIIVNVISDILGAMANPLKHKEWYALR"
     gene            16673..17563
                     /gene="sapC"
                     /locus_tag="OO357_000896"
     CDS             16673..17563
                     /gene="sapC"
                     /locus_tag="OO357_000896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease SapC"
                     /protein_id="HCR5885934.1"
                     /translation="MPYDSVYSEKRPPGTLRTAWRKFYSDASAMVGLYGCAGLAVLCI
                     FGGWFAPYGIDQQFLGYQLLPPSWSRYGEVSFFLGTDDLGRDVLSRLLSGAAPTVGGA
                     FVVTLAATICGLVLGTFAGATHGLRSAVLNHILDTLLAIPSLLLAIIVVAFAGPSLSH
                     AMFAVWLALLPRMVRSIYSMVHDELEKEYVIAARLDGASTLNILWFAVMPNITAGLVT
                     EITRALSMAILDIAALGFLDLGAQLPSPEWGAMLGDALELIYVAPWTVMLPGAAIMIS
                     VLLVNLLGDGVRRAIIAGVE"
     gene            17563..18555
                     /gene="sapD"
                     /locus_tag="OO357_000897"
     CDS             17563..18555
                     /gene="sapD"
                     /locus_tag="OO357_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapD"
                     /protein_id="HCR5885935.1"
                     /translation="MPLLDIRNLTIEFKTGDEWVKAVDRVSMTLTEGEIRGLVGESGS
                     GKSLIAKAICGVNKDNWRVTADRMRFDDIDLLRLSARERRKLVGHNVSMIFQEPQSCL
                     DPSERVGRQLMQNIPAWTYKGRWWQRFGWRKRRAIELLHRVGIKDHKDAMRSFPYELT
                     EGECQKVMIAIALANQPRLLIADEPTNSMEPTTQAQIFRLLTRLNQNSNTTILLISHD
                     LQMLSQWADKINVLYCGQTVETAPSKELVTMPHHPYTQALIRAIPDFGSAMPHKSRLN
                     TLPGAIPLLEQLPIGCRLGPRCPYAQRECIVTPRLTGAKNHLYACHFPLNMEKE"
     gene            18557..19363
                     /gene="sapF"
                     /locus_tag="OO357_000898"
     CDS             18557..19363
                     /gene="sapF"
                     /locus_tag="OO357_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein
                     SapF"
                     /protein_id="HCR5885936.1"
                     /translation="MIETLLEVRNLSKTFRYRTGWFRRQTVEAVKPLSFTLREGQTLA
                     IIGENGSGKSTLAKMLAGMIEPTSGELLIDDHPLHFGDYSFRSQRIRMIFQDPSTSLN
                     PRQRISQILDFPLRLNTDLEPEQRRKQIIETMRMVGLLPDHVSYYPHMLAPGQKQRLG
                     LARALILRPKVIIADEALASLDMSMRSQLINLMLELQEKQGISYIYVTQHIGMMKHIS
                     DQVLVMHQGEVVERGSTADVLASPLHELTKRLIAGHFGEALTADAWRKDR"
     gene            19431..19784
                     /locus_tag="OO357_000899"
     CDS             19431..19784
                     /locus_tag="OO357_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HCR5885937.1"
                     /translation="MMDKIKSNARDLRRNLTLQERKLWRYLRSRRFGDFKFRRQHPVG
                     SYILDFACCSARVVVELDGGQHDLAVAYDTRRTSWLESQGWTVLRFWNNEIDCNEEAV
                     LEIILQELNRRSPSP"
     gene            20152..20940
                     /gene="fabI"
                     /locus_tag="OO357_000900"
     CDS             20152..20940
                     /gene="fabI"
                     /locus_tag="OO357_000900"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HCR5885938.1"
                     /translation="MGFLSGKRILVTGVASKLSIAYGIAQAMHREGAELAFTYQNDKL
                     KGRVEEFAAQLGSDIVLQCDVAEDASIDTMFAELGKVWPKFDGFVHSIGFAPGDQLDG
                     DYVNAVTREGFKIAHDISSYSFVAMAKACRSMLNPGSALLTLSYLGAERAIPNYNVMG
                     LAKASLEANVRYMANAMGPEGVRVNAISAGPIRTLAASGIKDFRKMLAHCEAVTPIRR
                     TVTIEDVGNSAAFLCSDLSAGISGEVVHVDGGFSIAAMNELELK"
     gene            21084..22211
                     /locus_tag="OO357_000901"
     CDS             21084..22211
                     /locus_tag="OO357_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707196.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CMD domain-containing protein"
                     /protein_id="HCR5885939.1"
                     /translation="MEQRHITGKSHWYHETQSSTTEYDVLPLVPEAAKVSDPFLLDVI
                     LDEETLAPFLSWLVPARVLAVELFPDQLTVTRSQTFTAYERLSTALTVAQVCGVQRLC
                     NYYSARLTPLPGPDSSRESNHRLAQITQYARQLASSPSIIDNRSRQHLNDVGLTVWDC
                     VIINQIIGFIGFQARTIATFQAYLGHPVRWLPGLAIQNYADASLFADESIRWRSSYEV
                     EKLPEEHTKSSTAELCQLAETLSLHPISLSLLEKLLNSTRVNAQPDNQLAALLCARIN
                     GSPACFSTCMDSSNEYKKISTLLRKGENEINRWADRHSVERATVQAIQWLTRAPDRFS
                     AAQFSPLLEHEKSSTQIINLLVWSGLCGWINRLKIALGETY"
     gene            22279..24213
                     /gene="rnb"
                     /locus_tag="OO357_000902"
     CDS             22279..24213
                     /gene="rnb"
                     /locus_tag="OO357_000902"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exoribonuclease II"
                     /protein_id="HCR5885940.1"
                     /translation="MFQDNPLLAQLKQQLHSQTPRAEGVVKATEKGFGFLEVDAQKSY
                     FIPPPQMKKVMHGDRIIAVIHSEKERESAEPEELVEPFLTRFVGKVQGKNDRLTIVPD
                     HPLLKDAIPCRAARGLNHEFKEGDWAVAEMRRHPLKGDRSFYAELTQYITFGDDHFVP
                     WWVTLARHNLEKEAPDGVATEMLDEGLVREDLTSLDFVTIDSASTEDMDDALFAKALP
                     DDKLQLIVAIADPTAWIAEGSKLDKAAKIRAFTNYLPGFNIPMLPRELSDDLCSLRAN
                     EVRPVLACRMTLSADGTIEDNIEFFAATIESKAKLVYDQVSDWLENTGDWKPESEAIA
                     EQVRLLAQICQRRGEWRHNHALVFKDRPDYRFILGEKGEVLDIVAEPRRIANRIVEEA
                     MIAANICAARVLRDKLGFGIYNVHMGFDPANADALAALLKTHGLHVDAEEVLTLDGFC
                     KLRRELDAQPTGFLDSRIRRFQSFAEISSEPGPHFGLGLEAYATWTSPIRKYGDMINH
                     RLLKAVIKGETATRPQDEITVQMAERRRLNRMAERDVGDWLYARFLKDKAGTGTRFAA
                     EIVDISRGGMRVRLVDNGAIAFIPAPFLHAVRDELVCSQENGTVQIKGETVYKVTDVI
                     DVTIAEVRMETRSIIARPVA"
     gene            complement(24291..24394)
                     /locus_tag="OO357_000903"
                     /pseudo
     CDS             complement(24291..24394)
                     /locus_tag="OO357_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791239.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            24448..26422
                     /gene="pdeR"
                     /locus_tag="OO357_000904"
                     /pseudo
     CDS             24448..26422
                     /gene="pdeR"
                     /locus_tag="OO357_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309885.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
     gene            26570..26743
                     /locus_tag="OO357_000905"
     CDS             26570..26743
                     /locus_tag="OO357_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885941.1"
                     /translation="MSEFDAQRVAERIDIVLDILVAGDYHSAIHNLEILKAELLRQVA
                     ESTPDIPKTPWEI"
     gene            26833..27582
                     /locus_tag="OO357_000906"
     CDS             26833..27582
                     /locus_tag="OO357_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5885942.1"
                     /translation="MNSRQQTILQMVIDQGQVSVTDLAKATGVSEVTIRQDLNTLEKL
                     SYLRRAHGFAVSLDSDDVETRMMSNYTLKRELAEFAASLVQPGETIFIENGSSNALLA
                     RTLGEQKKNVTIITVSSYIAHLLKDAPCEVILLGGVYQKKSESMVGPLTRQCIQQVHF
                     SKAFIGIDGWQPETGFTGRDMMRTDVVNAVLEKECEAIVLTDSSKFGAVHSYSIGPVE
                     RFNRVITDSKIRASDLMHLEHSKLTVHVVDI"
     gene            27851..28069
                     /gene="osmB"
                     /locus_tag="OO357_000907"
     CDS             27851..28069
                     /gene="osmB"
                     /locus_tag="OO357_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmB"
                     /protein_id="HCR5885943.1"
                     /translation="MFVTSKKMTAAVLAITLAMSLSACSNWSKRDRNTAIGAGAGALG
                     GAVLTDGSTLGTLGGAAVGGVIGHQVGK"
     gene            complement(28195..28521)
                     /gene="yciH"
                     /locus_tag="OO357_000908"
     CDS             complement(28195..28521)
                     /gene="yciH"
                     /locus_tag="OO357_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response translation initiation inhibitor
                     YciH"
                     /protein_id="HCR5885944.1"
                     /translation="MSDSNSRLVYSTETGRIDEPKAAPVRPKGDGAVRIQRQTSGRKG
                     KGVCLITGIDLDDAELTKLAAELKKKCGCGGAVKDGVIEIQGDKRDLLKSLLEAKGMK
                     VKLAGG"
     gene            complement(28521..29258)
                     /gene="pyrF"
                     /locus_tag="OO357_000909"
     CDS             complement(28521..29258)
                     /gene="pyrF"
                     /locus_tag="OO357_000909"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HCR5885945.1"
                     /translation="MTLTASSSSRAVTNSPVVVALDYHNCDDALSFVDKIDPRDCRLK
                     VGKEMFTLFGPQFVRELQQRGFDIFLDLKFHDIPNTAAHAVAAAADLGVWMVNVHASG
                     GARMMTAAREALVPFGKDAPLLIAVTVLTSMEASDLADLGVTLSPADYAERLAALTQK
                     CDLDGVVCSAQEAVRFKQVFGQEFKLVTPGIRPQGSEAGDQRRIMTPEQALAAGVDYM
                     VIGRPVTQSVDPAQTLKAINASLQRSA"
     gene            complement(29451..30620)
                     /gene="lapB"
                     /locus_tag="OO357_000910"
     CDS             complement(29451..30620)
                     /gene="lapB"
                     /locus_tag="OO357_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapB"
                     /protein_id="HCR5885946.1"
                     /translation="MLELLFLLLPVAAAYGWYMGRRSAQQNKQDEANRLSRDYVAGVN
                     FLLSNQQDKAVDLFLDMLKEDTGTVEAHLTLGNLFRSRGEVDRAIRIHQTLMESASLT
                     YEQRLLAIQQLGRDYMAAGLYDRAEDMFNQLTDETDFRIGALQQLLQIYQATSEWQKA
                     IDVAERLVKLGKDKQRVEIAHFYCELALQHMASDDLDRAMTLLKKGAAADKNSARVSI
                     MMGRVFMAKGEYAKAVESLQRVISQDRELVSETLEMLQTCYQQLGKTAEWAEFLQRAV
                     EENTGADAELMLADIIEARDGSEAAQVYITRQLQRHPTMRVFHKLMDYHLNEAEEGRA
                     KESLMVLRDMVGEKVRSKPRYSCQKCGFTAYTLYWHCPSCRAWSTIKPIRGLDGL"
     gene            complement(30627..30935)
                     /gene="lapA"
                     /locus_tag="OO357_000911"
     CDS             complement(30627..30935)
                     /gene="lapA"
                     /locus_tag="OO357_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide assembly protein LapA"
                     /protein_id="HCR5885947.1"
                     /translation="MKYLLIFLLVLAIFVISVTLGAQNDQQVTFNYLLAQGEYRISTL
                     LAVLFAAGFAIGWLICGLFWLRVRVSLARAERKIKRLENQLSPATDVAVVPHSSAAKE
                     "
     gene            complement(31084..31848)
                     /gene="pgpB"
                     /locus_tag="OO357_000912"
     CDS             complement(31084..31848)
                     /gene="pgpB"
                     /locus_tag="OO357_000912"
                     /EC_number="3.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase B"
                     /protein_id="HCR5885948.1"
                     /translation="MRSIARRTAVGAALLLVMPVAVWISGWRWQPGEQSWLLKAAFWV
                     TETVTQPWGVITHLILFGWFLWCLRFRIKAAFVLFAILAAAILVGQGVKSWIKDKVQE
                     PRPFVIWLEKTHHIPVDEFYTLKRAERGNLVKEQLAEEKNIPQYLRSHWQKETGFAFP
                     SGHTMFAASWALLAVGLLWPRRRTLTIAILLVWATGVMGSRLLLGMHWPRDLVVATLI
                     SWALVAVATWLAQRICGPLTPPAEENREIAQREQES"
     gene            32018..32608
                     /gene="ribA"
                     /locus_tag="OO357_000913"
     CDS             32018..32608
                     /gene="ribA"
                     /locus_tag="OO357_000913"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HCR5885949.1"
                     /translation="MQLKRVAEAKLPTPWGDFLMVGFEELATGHDHVALVYGDISGHT
                     PVLARVHSECLTGDALFSLRCDCGFQLEAALTQIAEEGRGILLYHRQEGRNIGLLNKI
                     RAYALQDQGYDTVEANHQLGFAADERDFTLCADMFKLLGVNEVRLLTNNPKKVEILTE
                     AGINIVERVPLIVGRNPNNEHYLDTKAEKMGHLLNK"
     gene            complement(32672..35347)
                     /gene="acnA"
                     /locus_tag="OO357_000914"
     CDS             complement(32672..35347)
                     /gene="acnA"
                     /locus_tag="OO357_000914"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HCR5885950.1"
                     /translation="MSSTLREASKDTLQAKDKTYHYYSLPLAAKSLGDITRLPKSLKV
                     LLENLLRWQDGNSVTEEDIHALAGWLKNAHADREIAYRPARVLMQDFTGVPAVVDLAA
                     MREAVKRLGGDTAKVNPLSPVDLVIDHSVTVDRFGDDEAFEENVRLEMERNHERYVFL
                     KWGKQAFSRFSVVPPGTGICHQVNLEYLGKAVWSELQDGEWIAYPDTLVGTDSHTTMI
                     NGLGVLGWGVGGIEAEAAMLGQPVSMLIPDVVGFKLTGKLREGITATDLVLTVTQMLR
                     KHGVVGKFVEFYGDGLDSLPLADRATIANMSPEYGATCGFFPIDAVTLDYMRLSGRSE
                     DQVELVEKYAKAQGMWRNPGDEPIFTSMLELDMNDVEASLAGPKRPQDRVALPDVPKA
                     FAASNELKVNATHKDRLPVDYVMNGHQYQLPDGAVVIAAITSCTNTSNPSVLMAAGLL
                     AKKAVTLGLKRQPWVKASLAPGSKVVSDYLAKAKLTPYLDELGFNLVGYGCTTCIGNS
                     GPLPDPIETAIKKGDLTVGAVLSGNRNFEGRIHPLVKTNWLASPPLVVAYALAGNMNI
                     NLASEPIGHDRKGEPVYLKDIWPSAQEIARAVEQVSTEMFRKEYAEVFEGTAEWKEIN
                     VTRSDTYGWQEDSTYIRLSPFFDEMQATPAPVEDIHGARILAMLGDSVTTDHISPAGS
                     IKPDSPAGRYLQGRGVERKDFNSYGSRRGNHEVMMRGTFANIRIRNEMVPGVEGGMTR
                     HLPDSDVVSIYDAAMRYKQEQTPLAVIAGKEYGSGSSRDWAAKGPRLLGIRVVIAESF
                     ERIHRSNLIGMGILPLEFPQGVTRKTLGLTGEEKIDIGDLQNLQPGATVPVTLTRADG
                     SQEVVPCRCRIDTATELTYYQNDGILHYVIRNMLK"
     gene            complement(35511..35606)
                     /locus_tag="OO357_000915"
     CDS             complement(35511..35606)
                     /locus_tag="OO357_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885951.1"
                     /translation="MINTNMKYWSWMGAFSLSMLFWAELLWIITH"
     gene            complement(35720..35887)
                     /locus_tag="OO357_000916"
     CDS             complement(35720..35887)
                     /locus_tag="OO357_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_304581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885952.1"
                     /translation="MVGQEQLESSPLCQHSDNEPETKRECSVVIPDDWQLTSQQQAFI
                     ELFAEDDQPKQ"
     gene            complement(35890..36054)
                     /locus_tag="OO357_000917"
     CDS             complement(35890..36054)
                     /locus_tag="OO357_000917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmiA family putative membrane protein"
                     /protein_id="HCR5885953.1"
                     /translation="MLISDGDYMRLAMPSGNQEPRRDPELKRKAWLAVFLGSALFWVV
                     VALLIWKVWG"
     gene            complement(36349..37323)
                     /gene="cysB"
                     /locus_tag="OO357_000918"
     CDS             complement(36349..37323)
                     /gene="cysB"
                     /locus_tag="OO357_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012017585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator CysB"
                     /protein_id="HCR5885954.1"
                     /translation="MKLQQLRYIVEVVNHNLNVSSTAEGLYTSQPGISKQVRMLEDEL
                     GIQIFSRSGKHLTQVTPAGQEIIRIAREVLSKVDAIKSVAGEHTWPDKGSLYIATTHT
                     QARYALPNVIKGFIERYPRVSLHMHQGPPTQIADAVSKGNADFAIATEALHLYEDLVM
                     LPCYHWNRAIVVTPDHPLAGKKAITIEELAQYPLVTYTFGFTGRSELDTAFNRAGLTP
                     RIVFTATDADVIKTYVRLGLGVGVIASMAVDPVADPDLVRVDAHDIFSHSTTKIGFRR
                     STFLRSYMYDFIQRFAPHLTRDVVDAAVALRSNEEIEVMFKDIKLPEK"
     gene            complement(37533..40130)
                     /gene="topA"
                     /locus_tag="OO357_000919"
     CDS             complement(37533..40130)
                     /gene="topA"
                     /locus_tag="OO357_000919"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000513601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HCR5885955.1"
                     /translation="MGKALVIVESPAKAKTINKYLGCDYVVKSSVGHIRDLPTSGSAA
                     KKSADSTSTKTAKKPKKDERGALVNRMGVDPWHNWEAHYEVLPGKEKVVSELKQLAEK
                     ADHIYLATDLDREGEAIAWHLREVIGGDDARYSRVVFNEITKNAIRQAFNKPGELNID
                     RVNAQQARRFMDRVVGYMVSPLLWKKIARGLSAGRVQSVAVRLVVEREREIKAFVPEE
                     FWEVDASTTTPSGEALELQVTHQNDKPFRPVNKEQTQAAVSLLEKARYSVLEREDKPT
                     TSKPGAPFITSTLQQAASTRLGFGVKKTMMMAQRLYEAGYITYMRTDSTNLSQDAVNM
                     VRGYISDNFGKKYLPESPNQYASKENSQEAHEAIRPSDVNVMAESLKDMEADAQKLYQ
                     LIWRQFVACQMTPAKYDSTTLTVGAGDFRLKARGRILRFDGWTKVMPALRKGDEDRIL
                     PAVDKGDALTLVELTPAQHFTKPPARFSEASLVKELEKRGIGRPSTYASIISTIQDRG
                     YVRVENRRFYAEKMGEIVTDRLEENFRELMNYDFTAQMENSLDQVANHEAEWKAVLDH
                     FFSDFTQQLDKAEKDPEEGGMRPNQMVLTSIDCPTCGRKMGIRTASTGVFLGCSGYAL
                     PPKERCKTTINLVPENEVLNVLEGEDAETNALRAKRRCPKCGTAMDSYLIDPKRKLHV
                     CGNNPTCDGYEIEEGEFRIKGYDGPIVECEKCGSEMHLKMGRFGKYMACTNEECKNTR
                     KILRNGEVAPPKEDPVPLPELPCEKSDAYFVLRDGAAGVFLAANTFPKSRETRAPLVE
                     ELYRFRDRLPEKLRYLADAPQQDPEGNKTMVRFSRKTKQQYVSSEKDGKATGWSAFYV
                     DGKWVEGKK"
     gene            40510..40761
                     /locus_tag="OO357_000920"
     CDS             40510..40761
                     /locus_tag="OO357_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciN family protein"
                     /protein_id="HCR5885956.1"
                     /translation="MNKETQPIDRETLLKEANKIIREHEDTLAGIEATGVTQRNGVLV
                     FTGDYFLDEQGLPTAKSTAVFNMFKHLAHVLSEKYHLVD"
     gene            complement(40797..41846)
                     /gene="sohB"
                     /locus_tag="OO357_000921"
     CDS             complement(40797..41846)
                     /gene="sohB"
                     /locus_tag="OO357_000921"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease SohB"
                     /protein_id="HCR5885957.1"
                     /translation="MELLSEYGLFLAKIVTVVLAIAAIAAIIVNVAQRNKRQRGELRV
                     NNLSEQYKEMKEELAAALMDSHQQKQWHKAQKKKHKQEAKAAKAKAKLGEVATDSKPR
                     VWVLDFKGSMDAHEVNSLREEITAVLAAFKPQDQVVLRLESPGGMVHGYGLAASQLQR
                     LRDKNIPLTVTVDKVAASGGYMMACVADKIVSAPFAIVGSIGVVAQMPNFNRFLKSKD
                     IDIELHTAGQYKRTLTLLGENTEEGREKFREELNETHQLFKDFVKRMRPSLDIEQVAT
                     GEHWYGQQAVEKGLVDEINTSDEVILSLMEGREVVNVRYMQRKRLIDRFTGSAAESAD
                     RLLLRWWQRGQKPLM"
     gene            42066..42824
                     /locus_tag="OO357_000922"
     CDS             42066..42824
                     /locus_tag="OO357_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciK family oxidoreductase"
                     /protein_id="HCR5885958.1"
                     /translation="MHYQPKQDLLNDRIILVTGASDGIGREAAMTYARYGATVILLGR
                     NEEKLRQVASHINEETGRQPQWFILDLLTCTSENCQQLAQRIVVNYPRLDGVLHNAGL
                     LGDVCPMSEQNPQVWQDVMQINVNATFMLTQALLPLLLKSDAGSLVFTSSSVGRQGRA
                     NWGAYAASKFATEGMMQVLADEYQQRLRVNCINPGGTRTAMRASAFPTEDPQKLKTPA
                     DIMPLYLWLMGDDSRRKTGMTFDAQPGRKPGISQ"
     gene            42821..43411
                     /gene="cobO"
                     /locus_tag="OO357_000923"
     CDS             42821..43411
                     /gene="cobO"
                     /locus_tag="OO357_000923"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HCR5885959.1"
                     /translation="MSDERYQQRQQRVKEKVDARVAQAQDERGIIIVFTGNGKGKTTA
                     AFGTATRAVGHGKKVGVVQFIKGTWPNGERNLLEPHGVEFQVMATGFTWDTQNRESDT
                     AACREVWQHAKRMLADSSLDMVLLDELTYMVAYDYLPLEEVVQALNERPHQQTVIITG
                     RGCHRDILELADTVSELRPIRHAFDAGVKAQIGIDY"
     gene            complement(43451..44323)
                     /gene="rluB"
                     /locus_tag="OO357_000924"
     CDS             complement(43451..44323)
                     /gene="rluB"
                     /locus_tag="OO357_000924"
                     /EC_number="5.4.99.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2605) synthase RluB"
                     /protein_id="HCR5885960.1"
                     /translation="MSEKLQKVLARAGHGSRREIESIIEAGRVSVDGKIAKLGDRVEV
                     TPGLKIRIDGHLISVRESAEQICRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGAR
                     WIAVGRLDVNTCGLLLFTTDGELANRLMHPSREVEREYAVRVFGQVDDAKLRDLSRGV
                     QLEDGPAAFKTIKFSGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDI
                     PLPKGLPRGGWTELDLAQTNYLRELVELPPETSSKVAVEKDRRRMKANQIRRAVKRHS
                     QVSGSRRSGGRNNG"
     gene            complement(44424..45044)
                     /locus_tag="OO357_000925"
     CDS             complement(44424..45044)
                     /locus_tag="OO357_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HCR5885961.1"
                     /translation="MSQFFYIHPDNPQQRLINQAVEIVRKGGVIVYPTDSGYALGCKI
                     EDKNAMERICRIRQLPDGHNFTLMCRDLSELSTYSFVDNVAFRLMKNNTPGNYTFILK
                     GTKEVPRRLLQEKRKTIGMRVPSNPIAQALLEALGEPMLSTSLMLPGSEFTESDPEEI
                     KDRLEKQVDLIIHGGYLGQKPTTVIDLTDDTPVVVREGVGDVKPFL"
     gene            complement(45041..45922)
                     /gene="yciV"
                     /locus_tag="OO357_000926"
     CDS             complement(45041..45922)
                     /gene="yciV"
                     /locus_tag="OO357_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-3' exoribonuclease"
                     /protein_id="HCR5885962.1"
                     /translation="MSDTNYAVIYDLHSHTTASDGCLTPEALVHRAVEMRVGTLAITD
                     HDTTAAIAPAREEISRSGLALNLIPGVEISTVWENHEIHIVGLNIDITHPLMCEFLAQ
                     QTERRNQRAQQIAERLEKAQIPGALEGAQRLAQGGAVTRGHFARFLVECGKASSMADV
                     FKKYLARGKTGYVPPQWCTIEQAIDVIHHSGGKAVLAHPGRYNLSAKWLKRLVAHFAE
                     HHGDAMEVAQCQQSPNERTQLATLARQHHLWASQGSDFHQPCPWIELGRKLWLPAGVE
                     GVWQLWEQPQNTTEREL"
     gene            46060..46104
                     /gene="trpL"
                     /locus_tag="OO357_000927"
     CDS             46060..46104
                     /gene="trpL"
                     /locus_tag="OO357_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon leader peptide"
                     /protein_id="HCR5885963.1"
                     /translation="MKAIFVLKGWWRTS"
     misc_feature    46081..46174
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00513"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Trp leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00513"
     gene            46196..47758
                     /gene="trpE"
                     /locus_tag="OO357_000928"
     CDS             46196..47758
                     /gene="trpE"
                     /locus_tag="OO357_000928"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HCR5885964.1"
                     /translation="MQTQKPTLELLTCEGAYRDNPTALFHQLCGNRPATLLLESADID
                     SKDDLKSLLLIDSALRITALGDTVTIQALSGNGEALLALLDNALPAGVESEQSPNCRV
                     LRFPPVSPLLDEDARLCSLSVFDAFRLLQNLLNVPKEEREAMFFGGLFSYDLVAGFED
                     LPQLSAENNCPDFCFYLAETLMVIDHQKKSTRIQASLFAPNEEEKQRLTARLNELRQQ
                     LTEAAPPLPVVSVPHMRCECNQSDEEFGGVVRLLQKAIRAGEIFQVVPSRRFSLPCPS
                     PLAAYYVLKKSNPSPYMFFMQDNDFTLFGASPESSLKYDATSRQIEIYPIAGTRPRGR
                     RADGSLDRDLDSRIELEMRTDHKELSEHLMLVDLARNDLARICTPGSRYIADLTKVDR
                     YSYVMHLVSRVVGELRHDLDALHAYRACMNMGTLSGAPKVRAMQLIAEAEGRRRGSYG
                     GAVGYFTAHGDLDTCIVIRSALVENGIATVQAGAGVVLDSVPQSEADETRNKARAVLR
                     AIATAHHAQETF"
     gene            47758..49353
                     /gene="trpD"
                     /locus_tag="OO357_000929"
     CDS             47758..49353
                     /gene="trpD"
                     /locus_tag="OO357_000929"
                     /EC_number="2.4.2.18"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008784940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional anthranilate synthase glutamate
                     amidotransferase component TrpG/anthranilate
                     phosphoribosyltransferase TrpD"
                     /protein_id="HCR5885965.1"
                     /translation="MADILLLDNIDSFTYNLADQLRSNGHNVVIYRNHIPAQTLIERL
                     ATMSNPVLMLSPGPGVPSEAGCMPELLTRLRGKLPIIGICLGHQAIVEAYGGYVGQAG
                     EILHGKASSIEHDGQAMFAGLTNPLPVARYHSLVGSNIPAGLTINAHFNGMVMAVRHD
                     ADRVCGFQFHPESILTTQGARLLEQTLAWAQQKLEPANTLQPILEKLYQAQTLSQQES
                     HQLFSAVVRGELKPEQLAAALVSMKIRGEHPNEIAGAATALLENAAPFPRPDYLFADI
                     VGTGGDGSNSINISTASAFVAAACGLKVAKHGNRSVSSKSGSSDLLAAFGINLDMNAN
                     KSRQALDELGVCFLFAPKYHTGFRHAMPVRQQLKTRTLFNVLGPLINPAHPPLALIGV
                     YSPELVLPIAETLRVLGYQRAAVVHSGGMDEVSLHAPTIVAELHDGKIKSYQLTAEDF
                     GLTPYHQEQLAGGTPEENRDILTRLLQGKGDAAHEAAVAANVAMLMRLHGHEDLQANA
                     QTVLEVLRSGSAYDRVTALAARG"
     gene            49354..50715
                     /gene="trpCF"
                     /locus_tag="OO357_000930"
     CDS             49354..50715
                     /gene="trpCF"
                     /locus_tag="OO357_000930"
                     /EC_number="4.1.1.48"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional indole-3-glycerol-phosphate
                     synthase TrpC/phosphoribosylanthranilate isomerase TrpF"
                     /protein_id="HCR5885966.1"
                     /translation="MMQTVLAKIVADKAIWVETRKQQQPLASFQNEVQPSTRHFYDAL
                     QGARTAFILECKKASPSKGVIRDDFDPARIAAIYKHYASAISVLTDEKYFQGSFDFLL
                     IVSQIAPQPILCKDFIIDPYQIYLARYYQADACLLMLSVLDDDQYRQLAAVAHSLEMG
                     VLTEVSNEEELERAIALGAKVVGINNRDLRDLSIDLNRTRELAPKLGHNVTVISESGI
                     NTYAQVRELSHFANGFLIGSALMAHDDLHAAVRRVLLGENKVCGLTRGQDAKAAYDAG
                     AIYGGLIFVATSPRCVNVEQAQEVMAAAPLQYVGVFRNHDIADVVDKAKVLSLAAVQL
                     HGNEDQLYIDTLREALPAHVAIWKALSVGETLPAREFQHVDKYVLDNGQGGSGQRFDW
                     SLLNGQSLGNVLLAGGLGADNCVEAAQTGCAGLDFNSAVESQPGIKDARLLASVFQTL
                     RAY"
     gene            50727..51920
                     /gene="trpB"
                     /locus_tag="OO357_000931"
     CDS             50727..51920
                     /gene="trpB"
                     /locus_tag="OO357_000931"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HCR5885967.1"
                     /translation="MTTLLNPYFGEFGGMYVPQILMPALRQLEEAFVSAQKDPEFQAQ
                     FNDLLKNYAGRPTALTKCQNITAGTNTTLYLKREDLLHGGAHKTNQVLGQALLAKRMG
                     KTEIIAETGAGQHGVASALASALLGLKCRIYMGAKDVERQSPNVFRMRLMGAEVIPVH
                     SGSATLKDACNEALRDWSGSYETAHYMLGTAAGPHPYPTIVREFQRMIGEETKAQILE
                     REGRLPDAVIACVGGGSNAIGMFADFINETNVGLIGVEPGGHGIETGEHGAPLKHGRV
                     GIYFGMKAPMMQTEDGQIEESYSISAGLDFPSVGPQHAYLNSTGRADYVSITDDEALE
                     AFKTLCLHEGIIPALESSHALAHALKMMRENPDKEQLLVVNLSGRGDKDIFTVHDILK
                     ARGEI"
     gene            51920..52726
                     /gene="trpA"
                     /locus_tag="OO357_000932"
     CDS             51920..52726
                     /gene="trpA"
                     /locus_tag="OO357_000932"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HCR5885968.1"
                     /translation="MERYESLFAQLKERKEGAFVPFVTLGDPGIEQSLKIIDTLIEAG
                     ADALELGIPFSDPLADGPTIQNATLRAFAAGVTPAQCFEMLALIRQKHPTIPIGLLMY
                     ANLVFNKGIDEFYAQCEKVGVDSVLVADVPVEESAPFRQAALRHNVAPIFICPPNADD
                     DLLRQIASYGRGYTYLLSRAGVTGAENRAALPLNHLVAKLKEYNAAPPLQGFGISAPD
                     QVKAAIDAGAAGAISGSAIVKIIEQHINEPEKMLAALKVFVQPMKAATRS"
     gene            53106..53285
                     /locus_tag="OO357_000933"
     CDS             53106..53285
                     /locus_tag="OO357_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="general stress protein"
                     /protein_id="HCR5885969.1"
                     /translation="MAEHRGSSGNFAEDREKASDAGRKGGQHSGGNFKNDPQRASEAG
                     KKGGQQSGGNKSGKS"
     gene            53371..53871
                     /locus_tag="OO357_000934"
     CDS             53371..53871
                     /locus_tag="OO357_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5885970.1"
                     /translation="MNMKTIEDVFIQLLSDTYSAEKQLTRALAKLARATSNEKLSQAF
                     HAHLEETHGQIERIDQVVESESNLKIKRMKCVAMEGLIEEANEVIESTEKNEVRDAAL
                     IAAAQKVEHYEIASYGTLATLAEQLGYRKAAKLLKETLEEEKATDIKLTDLALNNVNK
                     KAENKA"
     gene            53917..54423
                     /locus_tag="OO357_000935"
     CDS             53917..54423
                     /locus_tag="OO357_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HCR5885971.1"
                     /translation="MNRIEHYHDWLRDAHAMEKQAESMLESMASRIDNYPELRARIEQ
                     HLSETKNQIVQLETILDRNDISRSVIKDSMSKMAALGQSIGGIFPSDEIVKGSIGGYV
                     FEQFEITCYTSLLAAAKNAGDTVSIPIIEAILNEEKQMADWLIQHIPQTTEKFLIRSE
                     TDGVEAKK"
     gene            complement(54483..55121)
                     /gene="ompW"
                     /locus_tag="OO357_000936"
     CDS             complement(54483..55121)
                     /gene="ompW"
                     /locus_tag="OO357_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpW"
                     /protein_id="HCR5885972.1"
                     /translation="MKKLTVAALAVTTLLSGSAFAHEAGEFFMRAGSATVRPTEGAGG
                     TLGSLGGFSVTNNTQLGLTFTYMATDNIGVELLAATPFRHKIGTRATGDIATVHHLPP
                     TLMAQWYFGDASSKFRPYVGAGINYTTFFDNGFNDHGKEAGLSDLSLKDSWGAAGQVG
                     VDYLINRDWLVNMSVWYMDIDTTANYKLGGAQQHDSVRLDPWVFMFSAGYRF"
     gene            55361..55450
                     /locus_tag="OO357_000937"
                     /pseudo
     CDS             55361..55450
                     /locus_tag="OO357_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_460692.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
     gene            55478..56221
                     /locus_tag="OO357_000938"
     CDS             55478..56221
                     /locus_tag="OO357_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0259 family protein"
                     /protein_id="HCR5885973.1"
                     /translation="MSITAQSVYRDTGNFFRNQFMTILLVSLLCAFITVVLGHVFSPS
                     DAQLAQLNDGVPVSGSSGLFDLVQNMSPEQQQILLQASAASTFSELIGNAILAGGVIL
                     IIQLVSAGQRVSALRAIGASAPILPKLFILIFLTTLLVQIGIMLVVVPGIIMAILLAL
                     APVMLVQDKMGIFASMRSSMRLTWANMRLVAPAVLSWLLAKTLLLLFASSFAALTPEI
                     GAVLANTLSNLISAILLIYLFRLYMLIRQ"
     gene            56251..56790
                     /locus_tag="OO357_000939"
     CDS             56251..56790
                     /locus_tag="OO357_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation protein A"
                     /protein_id="HCR5885974.1"
                     /translation="MKQFLDFLPLVVFFAFYKIYDIYAATAALIVATAIVLIYSWVRF
                     RKVEKMALITFVLVVVFGGLTLFFHNDEFIKWKVTVIYALFAGALLVSQWVMKKPLIQ
                     RMLSKELTLPQPVWSKLNLAWAVFFILCGLANIYIAFWLPQNIWVNFKVFGLTALTLI
                     FTLLSGIYIYRHMPQEDKS"
     gene            56895..57293
                     /gene="yciA"
                     /locus_tag="OO357_000940"
     CDS             56895..57293
                     /gene="yciA"
                     /locus_tag="OO357_000940"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioester hydrolase YciA"
                     /protein_id="HCR5885975.1"
                     /translation="MSTTHNVPQGDLVLRTLAMPADTNANGDIFGGWLMSQMDIGGAI
                     LAKEIAHGRVVTVRVEGMTFLRPVAVGDVVCCYARCVQKGTTSVSINIEVWVKKVASE
                     PIGQRYKATEALFKYVAVDPEGKPRALPVE"
     gene            complement(57333..58061)
                     /gene="tonB"
                     /locus_tag="OO357_000941"
     CDS             complement(57333..58061)
                     /gene="tonB"
                     /locus_tag="OO357_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB system transport protein TonB"
                     /protein_id="HCR5885976.1"
                     /translation="MTSITLDLPRRFPWPTLLSVCIHGAVVAGLLYTSVHQVIELPAP
                     AQPISVTMVAPADLEPPQAVQPPPEPVVEPEPEPEPIPEPPKEAPVVIEKPKPKPKPK
                     PKPVKKVQEQPKRDVKPVESRPASPFENTAPARPTSSTATAATSKPVTSVASGPRALS
                     RNQPQYPARAQALRIEGQVKVKFDVTPDGRVDNVQILSAKPANMFEREVKNAMRRWRY
                     EPGKPGSGIVVNILFKINGTTEIQ"
BASE COUNT        13921 a        15032 c        14531 g        14585 t
ORIGIN      
        1 aatgcacctt cctgctgtaa tgcctcgacc agcgctaata tttcgctttc tggaagcaag
       61 ccacgcagtg ggttcaaccc gatgccatcg tgagaggcga ggaagttaaa ccaggtcgtt
      121 ttactggagg gcaagctcag actttgcgcc cactggcaca gcgcctcgac gttctgtttt
      181 tgcaccgcat gtaacaccag cggcggcagc gagaactggt acaccatatg cgcttcgtca
      241 tcaccgttgc caaagtaagc aatgttgtct ttgtgcggaa cattggtttc ggtaataatc
      301 actgtacctg gtgctacgtc atcaataatc gaccgtaaca gcttgataat cagatgtgtt
      361 ttttccagat ggatgcagct tgttcccggc tctttccaca taaagccaac ggcatccagg
      421 cggacatact cagcaccttt ctcaaggtaa cagagtaaaa catccaccat cgccagcaac
      481 acttcagggc tacggtaatt caggtcaatt tgatcgtcac taaaggtggt ccataaatgg
      541 cgcgtggaat tatcatccat ttgaaacggc gttaatagcg gtaacgcacg cggaagggtg
      601 acggcactga gatcggtttt cggatcaacc gcaataaaga aatcttcaaa acccggaagc
      661 tgttgtaaat agtttttaaa ccattcactt tttgcagaca tatggttgca gacaaaatca
      721 aacattaaat gactgcattc accgagttgc tgaatatcct gccactcccc cgcttcactg
      781 gcgacctgat gataatcaat taccgaaaat ccatcatcag atgaccatgg gtaaaaaggt
      841 aataaatgaa catgtgaaaa aatgctttgc agccattgat ggtagaactg attaaatatg
      901 ggtagtggtt ttaaatcatt actgtgaaat tgatcggcat aggtaattaa cacgacatca
      961 ctttcatccc agtgtttttt acgtcgctgt gtaattaatc gtttcgcact ctcaagacgc
     1021 gttacaagtt tctgtaaatg agttgcggta aatgttccac cgtagatttc gtccatgtaa
     1081 tccgtaattt tctgtttcat gttgcctttc cgtggtagcg gtcccatgag gcgagagact
     1141 actccgcaga aaataaactg tcaattgagc acaagggttt tagtcacgca ggaaatcatt
     1201 ttttaccagg gaaaaagcgt atttgtgagc tgacgcaaat aatctatagg gatgtaaata
     1261 aggtgtgaca gaaaaaaaac ggcgcataag cgccgctcat ggtgaattct tttaaccttt
     1321 gaccttcggc attgcgatgt ctttcaggcc accggcgttc tcaacgtggg tatatcccat
     1381 ctcgctaagg agctcttttg cctgcccgga ctggcgtccg gcattgcaat acactttcac
     1441 ggtgtcgttt ttatccggaa cggcggtggc aatgcgctct ttcacttcct tcaggggaat
     1501 attgatggcc ccctgaacgt gctcttgctg atactgctct ggaacacgaa catcgatcca
     1561 gtgttcagcg gcgaaaacgg gcagtgaaaa caccagtgcc agagcaagta agcctttttt
     1621 taaacatgta agcgtccttc tatggatgaa aattgccatc cattatacgc ctgagattta
     1681 acaatttttg cgaaactccc ctcacctttt ataacgctgt gccagtttat tagcagcccg
     1741 acttaacagc ggttccagcg ccacagccag caacattttc agcggtcggc gagcaactga
     1801 ttttatcgcc cagcccgcca cacccgccgg gccatagcgc agtgcggtaa gaagtaccag
     1861 cttgcctgct aatttgaaac caagcttcac cttttgcccg gcctgttgcc agcgagtatt
     1921 cataactttc ctcacagttg acggaaacgg ctacgtaacg tgaaagtatc ggaagtgaca
     1981 taacgttcca tctcgcgtaa acgcgtttca cttgccgcca gttcacggtc gacttcatcc
     2041 agcaattcgc tgctggaagg tagctgctca ccaaaggcca tgttgtccgg cattggatca
     2101 agcgcaaatg acaaaatgat gtaagcaacc agggtaaaca gcgccagacc gaaaaaaatc
     2161 gacagcacca ccaggatacg caccagtttt accggtacat caaaatagtt ggcaatcccg
     2221 gcgcagacgc cgcggaccat gccctgctgt ggaatacgcc ataacttttt attgagatta
     2281 atgcccgcca ttagcgatcc ctccagttcg gatgttctgc atcaagaata gattccagcg
     2341 cctgaatacg ttcgcgcatc cgttttgctt catcaaccag ttgcgctaat cgctgctgct
     2401 cactttgcga caattcactg cgaccagaac gattgctgta atgcagccat aaccagatcg
     2461 gtaaaacaaa cagcacaaaa atggttaacg gaatagccag aaatagcgcg ctcataagta
     2521 ctccttagac gatgagcggc ggcgcgttcg gacgccgcct ggatgttatt attgattgtc
     2581 ttgcttcatt ttggctttta attgtgccag ttgttcgctg attgcatcat cggctttcag
     2641 ttcggcaaac tgatcgtcca gcgatttttg tttaccgaag ctgtggcttt ctgcttccgc
     2701 ttccatctgg tcaatacgac gttcgaaaga ttcgaaacga gccattgctt catccagttt
     2761 gccactgtcc agctgacgac gcacatcgcg cgacgagttt gccgcctgat gacgtaacat
     2821 caatgcctgc tggcgagcgc gtgtttcgct caatttgttt tccagctcgc caatctcttt
     2881 cttcatgcgt gccagcgtat cgtccaccag cgtcacttca tgttccaggg acttaatcag
     2941 atcggtcagt ttctgttttt caattaacgc tgcacgtgcc agatcctctc tctctttcag
     3001 cagcgccagt tcggcttttt cctgccattc aacttcacgc gccgacgctt gttcaatacg
     3061 gcgagtcagc tgtttctttt ctgccaacgc acgcgccgaa gtagaacgta cttcaaccag
     3121 tgtatcttcc atctcctgga tcatcagacg caccagtttc tgtggatctt ccgctttctc
     3181 taacagagcg ttgatgttgg cattcacgat gtcggcaaag cgagaaaaaa tacccataat
     3241 gttgtcctct tgatttctgc gttcaggatt gtcctgctga actgttaata caatttgcgt
     3301 gccaattttt tatctttttg atttataaag atctgattga agaatcaaca gcaacatgcc
     3361 aggatgaatt agcgaattac actaacaagt ggcgaatttc atcatggccg aatacaaaga
     3421 taatttactt ggtgaggcga acagctttct cgaagtgctg gaacaggttt cgcatctcgc
     3481 accgctggac aaaccggtgc tcatcatcgg cgaacgcggc accggtaaag agttgattgc
     3541 cagccgcctg cattatctct cctcccgttg gcaagggccg tttatttccc ttaactgcgc
     3601 ggcgttaaat gaaaatctgc tggattccga actgtttggt cacgaagcgg gggcgtttac
     3661 cggtgcgcaa aaacgtcatc ctgggagatt tgaacgtgcc gacggcggta cgctatttct
     3721 tgatgaactc gctacggcac cgatgatggt gcaggaaaaa ttattgcgtg tgattgagta
     3781 cggtgaactg gagcgcgttg gcggtagcca gccgttgcag gtgaatgtgc ggttggtatg
     3841 cgcgacgaat gccgatctcc cggcgatggt caatgaaggt acttttcgcg ctgacctgct
     3901 cgaccggctg gctttcgatg tagtgcaact gccgcccctg cgcgagcgcg aaagcgacat
     3961 tatgctgatg gcagaacact ttgccattca gatgtgtcgg gaaatcaagc tgcctctgtt
     4021 cccggggttt acggagcgcg ccagagaaac attgctgaat tatcgttggc cgggaaatat
     4081 tcgtgaattg aaaaacgtgg tggaacgttc agtgtatcgc cacggcacca gcgattatcc
     4141 gcttgatgac atcattattg atccctttaa acggcgtcca cctgaagaag ccatcgccgt
     4201 ttcagaaaat acctcgcttc cgacgctgcc gctggattta cgcgagtttc agatgcagca
     4261 ggaaaaagag ttgctgcaac tcagtttgca acaggggaaa tataaccaga agcgcgcggc
     4321 tgaattactg gggttaactt atcatcagtt ccgcgcgttg ttgaaaaagc accagattta
     4381 gcgaagttga gcaatacaac ttgccggatg cggcgtgaat gctttattcg gcatacataa
     4441 tcaatacgtc gtttgcaggc ctgataagcg cagcgcatca gacattattg cgttgggcta
     4501 ttaatcgctc agcgcatcct gcaaaatttt cattgccgca tcgaattgcg catccgggat
     4561 ggtcagcgga tacaggaagc gaatcacgtt gccgtatgcg ccacaggtca gcaggagcag
     4621 cccctgcgcc agcgcgcgtt gctggatttt ctgtgcaatc gccgctgacg gctcgcccgt
     4681 ttgcggatcg ttaaactctg ccgcaatcat cgaccccagg ccgcgtaccg cagcaatggc
     4741 cggaacgctt tctttggcat caatcaacgt gtttttgaga cgctggccca gttgattcgc
     4801 gcgttcgcag agtgattctt tgtcgataat attgagcacc gcgtgcgcgg cagccaccgc
     4861 cagcgggttc ccggcgtagg tgccacccaa cccgcccggc gcgggcgcgt ccataatatt
     4921 cgcgttaccg accacgcccg aaagcggcat cccgcccgcg aggcttttcg ccatcgtcat
     4981 taagtccggc ttatcggcgt aatgatccat ggcaaacagt ttaccggtac gcgcaaagcc
     5041 gctttgcact tcatcggcaa tcatcacaat accgtgctcg tcgcacaggc ggcgaatggc
     5101 ggcaaccagc tcttttggcg caacgttgaa accgcactcg ccctgcaccg gttcgaaaat
     5161 aatggccgcg acctgcttcg cttcgatgtc tgatttaaac aagcgttcga tggcgtcgag
     5221 ggagtcctgt gttgaaatgc cgtgtaaatc tgacggataa ggtacgtgat acaccgaacc
     5281 agggaacggg ccgaagccga ttttgtacgg cgcaactttt ccggtcagcg ccatggtcat
     5341 atatgtacga ccgtggaagc caccgctaaa cgcaatcacg ccaggtcgtc cggtatgggc
     5401 gcgagcaatt ttcaccgcgt tttccaccgc ttccgcgccg gtagtgaaga atgcggtttt
     5461 agcctgcccg ctcactgggg caagggtgtt aattttctcc gccagcgtga cgtagctttc
     5521 gtacggcaca atctgatacg ccgtgtgggt aaactgttgc aattgctgct ccaccgccgc
     5581 gaccagatca gggtggcgat gcccggtatt cagcaccgca atgcctgcgg cgaaatcgat
     5641 gtattcgttg ccctctacgt ccttcagcgt agcgttttct gccgactggg cgaagaagtt
     5701 acacatcacg ccaaccccgc gtggggtagc agaaagacga cgctgatgga attcattgtt
     5761 gctcatgttt cacacctttt gactcttcac tgttaatcgg aaattaaacg cccagacgat
     5821 cgcgcaggct gtaataagcc gcgcccatcg cggtaaatgg cacgcgcagc gtgcgcccgc
     5881 cggggaacgg gtaatgcggc agattggcga aggcatcgaa acgctcggcg tcaccgcgca
     5941 gcagttcagc aatcaaacgc ccggcaagat gggtacaggt gacgccgtgg ccgctgtaac
     6001 cctgcatgta atagatgttg gtatcaaggc ggccgaactg cggcatgtgc gacaaggtca
     6061 gcaggaagtt gcccgtccag cggtaatcaa ttttcacgcc cttcaactga gggaaggttt
     6121 ttagtagttt cggtaccaca agacgctcaa cgtcatccgg gttgcgtgca ccatacacta
     6181 cacccccgcc gtacagcagg cggttgtcgg cggtaagacg gtagtaatca agcagatagt
     6241 tacagtcttc cacacagtag tttttcggga tcagcgaacg ggctaaatct tccgacagcc
     6301 gttcggtggt gatcacctgc gtgccgcacg gcatgctgcg tttcgctagt tccggctcta
     6361 ctttatcgcc cagatacgca ttcccggcga caatcacata cttcgccgtc acctgaccat
     6421 tggcagtccg aaccacggct ggcgtggtgt gctggatctg cgtcacggcg gaaagctcat
     6481 acacgcgccc gccgttcagg cggatggcat cggcttcacc aatggcgagg tttagcgggt
     6541 gaatatgccc accgctgtga tccagcagcg caccagtgta gcgatcgctg gcgacctcgc
     6601 ggcgaatagc gttgacgtcc agcagctcca gctgtttatt gccgtagcgt acccagttct
     6661 ctttctgctc ttcgagtgtg gcgagttgtt tatcattcat cgccacaaac agaccgccgg
     6721 ggcggtagtc gcagtcaatc tgataacgtt tgatacgttc gcggatgatc tcgccaccct
     6781 cgaacatcat gctgccgagc atacgggcgg tgtccatgcc gtagcttttt tcgatcacgt
     6841 cgatgtcgcg gctgtaggag ttcacaagct gcccgccatt gcgcccgctt gcgccaaagc
     6901 cgatgcgtga ggcttcgaga accactacgt caaagcccgc ttccgccaga tgcagcgcgg
     6961 aggagagccc ggtatagccg ccgccaacca cgcaaacgtc gcaggtgatt gactcattca
     7021 gtgtgtcgaa tggtgcatat ttattcgcac tggcggcgta gtaactgctg gtatgttcgg
     7081 tcatttcagg cctccaggct tatccagatg gttttcagtt cagtgaattt ttcaagggca
     7141 tgcagggatt tgtcgcgacc gttgccgctc tgcttatagc cgccaaacgg cacggtcata
     7201 tcgccgtcgt tgtagttatt gacgaagacg gaaccggctt tcaggcgtcg gctcatgcgg
     7261 tgcgcgcggg agaggtcgcg cgtccatacc gccgcgccaa ggccgtactg gctgtcgttg
     7321 gcaagctgca gcgcctgatc ttctgatgtg aaacgggtga ccaccagcac cggaccgaaa
     7381 atctcttcgc ggcttaagga cgcattcggg tccacatcca cgaagatggt cgggccgatg
     7441 gccgctgcca gcccggcgtt gcgaccatcc agcagcagtt gccccttgcc ttcgccttca
     7501 cgaataaagg tgtgtaccga gtcagcgtga gcgctgtcga ttaaggtgcc catggtggtt
     7561 gcgggatcaa gtggatggcc cggctgccag ttttgcgcct gctgttttaa cagggctaag
     7621 aattcatcgg cgatgctctc ttccagtaac aaacgcgttc cggcgatgcg cacctggccc
     7681 tggttgtaga agatgcctgc ggcggtggcg cttgccgctt tttgcagatc cggacaatcg
     7741 gcgaaaacga tgttggcgct tttgccgccc gcttccagcc agacacgttt catgttgcta
     7801 tcacccgcgt ctttcagcag ttgtttaccc gtacgcgtgg agccggtaaa ggcgatggca
     7861 tcgatatcgt tatgacgcga aagcgcctgc cccgcttcat gaccaaaacc cgtcaccacg
     7921 ttcaacacac catccggcaa gcctgcttct ttcgccagcc ccgcgagacg aatcgcactg
     7981 agcggtgatt tttcagacgg ttttagaatc acgctgtttc ccgccgccag cgccgggccg
     8041 agtttccagc aagtcagcaa cagcgggaag ttccacggca cgatggcggc aatcacgccg
     8101 accggttcac gcacgatcat cgccagctca tggctactgg tggtcgccac ttcgccatac
     8161 actttgtcga tcgcttcggc gtaccagcga atggcgcgcg ccgcgccggg aatatcatca
     8221 cgcagactgt gacgaatcgg tttgccggtg tcgagagttt ccagcagtgc cagctcttcg
     8281 gcgtgggctt ccattaaatc ggcgagttta ttcagtaccg ctttacgttt agccggagaa
     8341 gagagtgacc agtcgccgcg ttcaaatacg ccgcgtgctg cgctcaccgc acggtcgata
     8401 tcgacgctct tgccgcgggc aattttcgcc agcggtgcct gggtgaccgg atcaacggtt
     8461 tcaaaggttt cattttccgc cgcagcagta tattcaccgt taataaataa gcggttttca
     8521 atggcgagac ttaacgcttt atcctgccag taagccagat gatgaaaatt cattatgact
     8581 cctgtttcac gtctatcaga tatatgcagt ttgtgaacgt cagggcgcag agatccccgg
     8641 gctgcatgcc cgtttatttc tggcgacatt ctttatggat tgccgggcgc attgcgctta
     8701 cccggcttac agaacaaaaa gattaaaacg tggtgggcgt atgggcgctg ataattcggc
     8761 aaatacctgc cgacgtatta ctgaaactgt gcgggatgcc ggtattaatg gcataacttt
     8821 gccccgcgac gaggtggtaa tcctgaccat taatcgtcag aacaatttca ccttccagta
     8881 ctgtgcctat ttcctcaccc tgatgcttaa ttctttcccc agtggttgtg cccggctggt
     8941 acgtttcaaa gatcatcgcc agcgtgcgat tcgggttacc gttatgaacc agcttcattg
     9001 acacaccctg actgcccatc tcaattaagt cgtcctgatt aatgacgacc tgcggctcat
     9061 caggtttttc cggctcggaa aagaattccg agagtgacag accatacacc ttcagcagct
     9121 tttgcagcgt actgatggca gggctgactt tatcttgttc tatcgtactg atagcactgt
     9181 gagtcagccc ggagagttcg gcggcacgac gttgtgaaag cccctgctgc tggcggattt
     9241 ccgacaagcg ttttcctggc gccagtccct catcactcat atttgcattt ccttaaactg
     9301 tagtggtcag agtcgctgtt tttcagcgat atggtgctgc caagcggtga taaagccctc
     9361 gaacaatata cgcgaaagcg cgtactcgct actgttccat tccgggtgcc actgtacgcc
     9421 cagcgcaaaa ggatgattga tgacgctaac cgcctcaact aagccatccg gcgagcgagc
     9481 ttcaacacgc aaccgtgggc taacgacctt cgccccttgt ccatgtagag agtttaccca
     9541 aaagttgcta cattcaggta acaacgcaga cagtaatccc ccctcttcaa cctgaacttc
     9601 atgcgacggt gcatattgct gttccaccgg cagttcggga tcttcccgat gttccagcaa
     9661 ttcaggctgt tcgcacagct tgcgatgcaa cgaaccaccg gttgccacca ccagttcttg
     9721 taaaccccgg cagatggcga aaatggggat gcgcctttcg agtgcggcat taattatcgc
     9781 catgctcaga agatcacgcc cgggatcggc gtcaggctca tcgccgtttt caccatatag
     9841 gtgcggctgc acattgctgg gactactagg aagataaatg ccatcgagtt tcggcaaaag
     9901 ttgttcaagt aacgacggtt ccgccagcgc atgtggtagc gcaattggca agccgcctgc
     9961 gtggatgatg gcattcaggt acttttcttg cagagtctgg gtcgcatgac ccttaagcct
    10021 gttcctgcac attacgacac cgataaccgg attgttcatt atattttcca tgctcaatct
    10081 cacaaaatgg actaaattat cgccattact gcgctttaaa tgacctttat gttcaatatt
    10141 ttttcaatct agcagtggat tttcattttt gcaaactcaa tttaacattt gacaaacatt
    10201 tggtttgcat acagattcga atgatggtca ttatatttta cgctttgata acgagcggaa
    10261 aacaaaccaa aggcgaagaa tcatggaaac caatatcgtt gaagtagaga actttgttca
    10321 gcagtcagaa gagaggcggg gtagcgcctt tacgcaggaa gtgaaacgct acctggagcg
    10381 ctacccgaat acgcaatatg ttgatgtttt acttactgat ttgaatggtt gcttccgtgg
    10441 aaaacgtatc cctgtttcaa gcctgaagaa gctcgagaaa gggtgttatt tcccggcctc
    10501 ggtatttgca atggatattc tgggcaacgt agtagaagaa gcaggtctgg gtcaggaaat
    10561 gggcgagccg gatcgtacct gtgttcctgt tctcggttcc ttaactcctt ctgccgccga
    10621 tccagagttt atcggtcaga tgctcctgac catggtcgat gaagatggcg ctccctttga
    10681 cgttgagccg cggaacgttc ttaaccgcct gtggcagcag ctgcgccagc gcggattgtt
    10741 cccggttgta gcggtagagc tggagttcta tttactggat cgccagcgcg acgctgaagg
    10801 ggatctgcaa ccgccctgcg cgcctggcac cgatgaccgc aatacacaaa gccaggttta
    10861 ctccgttgat aacctcaacc acttcgccga cgtgctcaat gatattgatg aactggcgca
    10921 gttgcagctg attccggcag atggcgcggt cgctgaggcc tcgccgggtc agtttgaaat
    10981 caacctttac catactgata acgtgctgga agcctgcgat gatgcgctgg cactaaaacg
    11041 cctagtgcgt ctgatggcag aaaagcataa gatgcacgcc acttttatgg cgaagccgta
    11101 tgaagagcac gcgggcagcg ggatgcatat ccatatcagt atgcaaaata atcgtggcga
    11161 gaatgtgctt tctgacacag aaggcgaaga ttcgccgctg ctgaaaaaga tgctcgccgg
    11221 gatgattgac ctgatgccgt cgtcgatggc gttgctggca ccaaacgtga actcgtatcg
    11281 ccgcttccag ccgggaatgt atgtgccgac gcaggcgtcg tggggccata acaaccgcac
    11341 cgtcgccctg cgtattccgt gcggtgaccg tcataatcac cgcgtggaat atcacgtggc
    11401 gggtgccgat gccaacccat atctggtgat ggcagcgatt tttgccggta ttttgcatgg
    11461 ccttgataac gagctgccgt tgcaggaaga agtcgaaggc aacgggctgg aacaggaagg
    11521 cttacccttc ccgattcgcc agagcgatgc cctgggtgag tttatcgaga atgatcacct
    11581 gcgccgctat ttaggcgaac ggttttgcca tgtgtatcac gcctgtaaaa atgatgagct
    11641 gttgcagttt gagcgcctca tcacagaaac agaaattgag tggatgttga aaaacgcgta
    11701 attccccttg cggggttgtt tcatcggcct gagcggagcc aggcctgaca tttgctggta
    11761 atccagacat ttcccggatg tcgcgttatg gagcgcaggc ggcaacgggc ttccgttaac
    11821 gattaagact gtgacgagga aatgagatga tgccgatgat taaatccccg catggcgagg
    11881 gaggctgcgt atgcgcccct cctgctactg actggactcc gccccctttg cttcccttgc
    11941 taaacaggtt tgatttcagg tcgacacgac cgcaaacctt attacgcagg ggaggcagca
    12001 attatggcta ttaattcacc actgaatatt gctgcgcaac ccggcaaaac ccgtctgcga
    12061 aaatcactga aattgtggca ggtggtaatg atgggtctgg cctatctcac gccgatgacc
    12121 gtatttgata cttttggtat tgtctccggc attagcgacg gtcacgttcc ggcgtcctat
    12181 ttgctggcgc tggcgggcgt gctgtttacc gctatcagct acggcaaact ggttcgccag
    12241 tttccggagg ccggttcggc ctatacctac gcgcaaaagt cgattaaccc gcacgtcgga
    12301 tttatggtcg gctggtcatc actgctggat tatctctttt tgccaatgat caacgttttg
    12361 ttggcgaaaa tctatctctc cgccctcttc ccggaagtgc cgccgtgggt gtgggtggta
    12421 accttcgtcg ccattttaac cgccgcgaat ctgaagagcg tcaacctggt cgctaacttc
    12481 aataccctgt ttgtactggt gcaaatctcc atcatggtgg tgtttatctt cctggtggtt
    12541 cagggactgc ataaaggaga aggcgttggc accgtctggt cacttcagcc gtttatcagc
    12601 gagaacgcgc acctgatccc gattattacc ggtgcgacga ttgtctgttt ctcgttcctc
    12661 ggtttcgatg cggtgaccac gctttcggaa gagacgccgg acgccgcacg cgtgatcccg
    12721 aaagccatct tcctgacggc ggtatacggc ggcgttatct ttatcgcggc gtcgttcttt
    12781 atgcagctgt tctttcccga tatcagccgc tttaaagacc cggacgccgc actgcctgaa
    12841 attgcgctgt acgtcggcgg caagctcttt cagtcgattt tcctctgcac cacgtttgtg
    12901 aacacattag cgtctggcct ggcttcacat gccagcgtgt cgcgtctgct gtatgtgatg
    12961 gggcgtgaca atgtgtttcc ggagcgcgtg tttggctatg tgcacccaaa atggcggact
    13021 ccggcactca acgtcattat ggtcgggatt gtcgcgctgt cggcgctgtt ctttgattta
    13081 gtcaccgcga cagcactgat taacttcggt gctctggtgg cgtttacctt cgtgaatctg
    13141 tcggtgttta atcatttctg gcggcgtaaa ggaatgaata aaagctggaa ggatcacttc
    13201 cactatttgc tgatgccgct ggttggcgcg ctgacggtgg gtgtgctgtg ggttaaccta
    13261 gagtcaacgt cactgacact cggtctggta tgggcttcgt taggcggcgc atatttgtgg
    13321 tatctgatcc gccgctatcg caaagtgccg ctgtacgaag gtgacagaac gccggtgagc
    13381 gaaacgtaat agccggatgc gcggcgcatt atcctgcctg agggtaacgc gccgcgccat
    13441 taactataaa aagatgtatg acccggatca cgagtgctat catccgccct tatgcttagc
    13501 gcattaaccc caggggcaaa taatgaacca cgatatcccg ctaaaatatt ttgatattgc
    13561 cgatgagtac gcgaccgaat gcgcagagcc tgtcgcagag gctgaacgca caccgctggc
    13621 ccactacttc cagttactgc tcacccgttt gatgaacaat gaagagatca gcgaagaagc
    13681 ccaacatgaa atggctgctg aagcggggat taatcccgta cgcattgatg agatcgcaga
    13741 gtttttgaat caatggggta atgagtaatt atttaattgc agacgatcgg tcagtatcgt
    13801 tgtccggata aagcgttcac gccgcatccg gctaacaatg acacgcactg cagcgtgctt
    13861 taatgggttt ctcacgatcg taccgctctg ctcctcgcta agttgttctg tgagttatag
    13921 ttatagagaa atcacgggta cgaatgttga acagatggcg acgccacctg ttcaacgacc
    13981 aaccacacgg ccctcattac gacccctgaa cacaaacggt aatggcaaat gtcattaaat
    14041 attagtaaga tagcctcttt acataaaatc ccttaatatt atgcgccagg tattatcgtc
    14101 tcttttggtg attgctggac ttgtgagtgg tcaggcaatc gccgcgcctg aatccccccc
    14161 gcatgctgat atccgcgaca gcggttttgt ctattgcgtc agcgggcaag tcaacacctt
    14221 taacccatcc aaagcgagca gtgggttaat tgtcgatacc cttgccgccc agttttatga
    14281 tcgactgctg gatgtcgatc cctataccta tcgcctgatg ccggaacttg ccgaaagctg
    14341 ggaagtactc gacaacggcg cgacctatcg cttccacctg cgtcgcgatg ttccgtttca
    14401 aaaaaccgac tggtttactc ccactcgtaa aatgaatgcc gacgatgtgg tgtttacctt
    14461 ccagcgaatt tttgaccgca acaacccgtg gcataacgtc aacggcagca acttccccta
    14521 cttcgacagc ctgcaatttg ccgataacgt gaaaagcgtc cgcaaactgg ataatcatac
    14581 cgttgagttc cgactcgccc agccggatgc ttcttttttg tggcacctcg caacccatta
    14641 tgcttcggtc atgtcggcag aatatgcccg gaagttagag aaagaagatc gccaggagca
    14701 actcgaccgt caaccggtcg gcaccggtcc gtatcagttg tcggaatacc gcgccgggca
    14761 atttattcgc ctacaacgtc atgatgactt ctggcgcggt aaaccgttaa tgccgcaggt
    14821 agtggtggat ttaggctccg gcggcaccgg acgtctgtcg aaactcctga ccggggaatg
    14881 cgacgttctg gcctggcctg ctgccagcca gctatccatt ttgcgtgacg acccgcgctt
    14941 gcgtttaacg ctgcgtcctg ggatgaacgt cgcctatctg gcatttaaca ccgccaaacc
    15001 gccgctaaat aatcccgctg tccgccatgc actggcactg gcgattaata accagcgcct
    15061 gatgcaatcc atctattatg gtacggctga aacggcggcc tctattttac cgcgcgcctc
    15121 gtgggcctat gacaacgagg ctaaaattac tgaatacaat ccggcgaaat cgcgcgaaca
    15181 gttgaaggcg ttggggctgg aaaacttaac gctgaaactg tgggtgccca cacgttcgca
    15241 ggcgtggaac cccagtccac tgaaaactgc cgaactgatt caggcggata tggcgcaggt
    15301 tggcgtaaaa gtggtgattg tgccggtaga aggtcgcttt caggaggcgc ggttgatgga
    15361 tatgagccat gatctgacgt tatccggttg ggcgacggac agtaacgacc cggacagttt
    15421 cttccgtccg ttactgagct gcgcggcaat tcattcacag accaacctcg cccactggtg
    15481 caatccgaaa ttcgacagcg tgttgcgtaa ggcgctctcc tcgcagcagc tggcagcgcg
    15541 tattgaagcc tatgacgaag cgcagagtat tctggcgcag gaattaccta ttctgccgct
    15601 ggcgtcgtca ttgcgtttgc aggcctatcg gtacgatatc aaaggtctgg tacttagccc
    15661 gtttggtaac gcctcctttg ctggggtgta tcgcgagaaa caggatgagg tgaaaaaacc
    15721 atgattatct tcaccttacg ccgcattttg ttattgattg tcaccttgtt cctgctgacc
    15781 ttcgttggct tcagcttgag ctatttcact ccacacgcgc cgttacaagg cgcgtcactg
    15841 tggaatgcct gggtgttctg gtttaatggc ctgatccact gggattttgg tgtttccagc
    15901 attaacggtc aaccaattgc cgaacagtta aaagagctct tcccggcgac gatagaactg
    15961 tgcatcctcg ccttcggttt tgcgctgatt gttggcatcc ccgtggggat gattgccggg
    16021 attacgcgcc ataagtggca ggataacttg atcaacgcca tcgccttact gggcttttca
    16081 atcccggtgt tctggctggc gcttctgttg acactgtttt gttcactcac gctaggttgg
    16141 ttgcccgttt cagggcgttt cgatctgctc tacgaagtga aaccgattac cggttttgcg
    16201 ttgattgatg cctggctttc ggactcacca tggcgggatg aaatgatcat gagcgcaatc
    16261 cgccatatga tattgcccgt gatcaccctg tcggttgcgc caacaactga agtgatccgc
    16321 ctgatgcgta tcagcactat tgaagtgtat gaccagaact atgtaaaagc ggcggcgacc
    16381 cgcggtttgt cgcgctttac tattttgcgt cgccacgttc tgcataacgc gttaccgccg
    16441 gttattcctc gcctgggctt acagttttcc accatgctga cgctggcgat gatcaccgaa
    16501 atggttttta gctggccggg tttagggcgc tggttaatta acgccatccg ccagcaggat
    16561 tacgcagcca tttccgccgg agtgatggtg tgtggctcac tggtcattat tgttaacgtg
    16621 atttctgata ttttgggtgc catggctaac cctctgaaac ataaggaatg gtatgcctta
    16681 cgatagcgta tacagcgaaa agcgcccgcc gggcacgctg cgtaccgcct ggcgcaaatt
    16741 ttatagtgac gcctctgcga tggtcggcct gtacggctgc gcgggactgg ctgtactgtg
    16801 tatttttggc ggctggtttg cgccttacgg catcgaccag caatttctcg gttatcaatt
    16861 attgccgcca tcatggtcgc gctatggcga agtttctttc ttcctgggga ctgacgacct
    16921 cggacgtgat gttttaagcc gtttactgag cggagctgcg ccaaccgtgg gtggcgcatt
    16981 tgtggtgacg cttgccgcga cgatctgtgg cctggtactg ggcaccttcg ccggggcgac
    17041 acacggcctc cgctcagcgg tgcttaacca tattctggat accttactgg cgatcccttc
    17101 gctgctgttg gcaattatcg ttgtagcgtt tgccggaccg agtttgtccc acgctatgtt
    17161 tgctgtctgg ctggcattgc tgccgcgtat ggtacgttcg atttacagca tggtgcatga
    17221 cgaactggaa aaagagtacg ttatcgccgc ccgtctggat ggcgcatcaa cgctgaatat
    17281 tctctggttt gctgtgatgc caaacatcac cgccgggctg gtcaccgaga tcacccgcgc
    17341 actgtcgatg gcaattctcg atatcgccgc gctgggcttt ctcgatctcg gcgcacaact
    17401 cccctcgcct gaatggggag cgatgctcgg tgatgcgctg gaactgattt atgtcgcccc
    17461 gtggactgtc atgctgccag gtgcggcaat tatgattagc gtgttgttgg ttaacctcct
    17521 tggcgatggc gttcgtcgtg cgattattgc gggggtggaa taatgccatt actcgatatt
    17581 cgtaacctga ccattgaatt taaaaccggt gatgagtggg ttaaagccgt cgaccgggta
    17641 agtatgacct taaccgaagg tgaaatccgc ggtcttgttg gtgaatccgg ttccggcaag
    17701 agtttgattg cgaaagcgat ttgtggggtg aataaagata actggcgtgt tactgctgac
    17761 cgtatgcgtt ttgatgatat cgatttgctg cgtctctccg cacgcgaacg gcgcaaactg
    17821 gttggccata acgtgtcgat gattttccag gaaccgcagt cgtgtcttga cccttcagaa
    17881 cgtgtgggtc gccagttgat gcaaaacatc ccagcctgga cctataaagg ccgttggtgg
    17941 cagcgttttg gctggcgcaa acgccgtgcc attgaactgc tgcaccgcgt ggggattaaa
    18001 gatcacaaag atgcgatgcg cagttttccc tatgagttga ccgaaggtga atgtcagaaa
    18061 gtgatgatag ccattgcact ggcgaatcaa ccgcgtctgc tgattgctga cgaaccgaca
    18121 aactcaatgg agccaacaac ccaggcgcaa atctttcgcc tgctgacgcg tctcaaccaa
    18181 aacagcaata ccactatttt gcttatcagc catgacttac aaatgcttag ccaatgggcg
    18241 gataaaatta acgtgcttta ctgcggtcaa acagtggaaa ccgcgccgag taaggagttg
    18301 gtgacgatgc cacatcatcc ttatacccag gcgctgatcc gcgcgatacc agacttcggc
    18361 agcgcgatgc cgcataaaag tcgcctcaat actctacccg gcgctatccc attgctggaa
    18421 cagttaccga ttggttgtcg tctggggcca cgttgcccgt atgcacaacg agaatgcatt
    18481 gtgacgccac gtttgacggg ggcgaaaaat catctctatg cctgtcattt cccgctgaac
    18541 atggagaaag agtgagatga tcgaaacgct gcttgaagtg cgtaatttaa gtaaaacgtt
    18601 ccgctaccgg accggttggt ttcgtcgtca gaccgtagaa gcggtaaaac ccttgagctt
    18661 tacgctacgt gaaggccaga cactggcgat tattggcgag aatggttcgg gtaaatccac
    18721 actggcaaaa atgctggcgg gaatgataga acctaccagc ggcgagttat tgattgacga
    18781 tcatccgctg cattttggcg attattcctt ccgtagtcag cgtattcgca tgatttttca
    18841 ggatccttcg acctcgttga atccccgtca acgtatctca caaattctgg attttccact
    18901 gcgcctgaac accgacctgg aaccggaaca gcgtcgcaaa cagattattg aaacgatgcg
    18961 tatggttggg ctgctaccgg atcacgtcag ttactatccg catatgttgg cacccggaca
    19021 aaaacagcgt ctgggtctgg cgcgcgcgct gatattgcgc ccaaaagtca ttattgccga
    19081 tgaagccctc gcttcactgg atatgtcaat gcgttcgcag ttgattaatc tgatgctgga
    19141 gttacaggaa aaacagggca tttcgtatat ttatgttacc cagcatatcg gaatgatgaa
    19201 gcacattagc gaccaggtgc tggtgatgca tcagggcgag gttgtcgagc gaggcagcac
    19261 cgcggatgtg ctggcatcgc cgctgcatga actcaccaaa cggctgattg ccgggcactt
    19321 tggcgaagca ttgacagcgg atgcgtggcg taaagatcgc taattttctg aatcctcctt
    19381 ccagattatt cacctcattc tgacaagaga ttaaatcttg tcagaatgag gtgatggata
    19441 aaataaaatc aaatgcgcgt gatttaagac gcaatctcac tttgcaggaa cgaaagctct
    19501 ggcgatatct tcgcagccga cgttttggtg atttcaaatt tcgccgtcaa catccggtgg
    19561 ggagctacat tctcgatttt gcttgctgct cggcgcgcgt agtcgttgag ctggatggtg
    19621 ggcagcatga tttggcagtt gcctatgata ccaggcgcac tagctggctt gagtcgcagg
    19681 gctggaccgt gctgcgtttc tggaataacg agattgattg taatgaggag gcggtgctgg
    19741 agattattct gcaggaactg aaccgccggt caccctcacc ctgaaagggc gagggggccg
    19801 accgagccga atagctgttg tggtgaaaac atggagacag tgctggagaa tattcggcaa
    19861 ggtctgaacc atcccagcca tcgccataaa agggttaggg gctgtatgag cctgtttgtt
    19921 gctggggtaa caatatttgc acaatatggt cccctcgccc ctctggggag agggttaggg
    19981 taaggggaaa agcgcccccc ctgccgcagc ctgctccggt cggacctggt aactatagct
    20041 actcacagcc aggttgatta taataaccgt ttatctgttc gtactgttta ctaaaacgac
    20101 gaatcgcctg attttcaggc acaacaagca tcaacaataa ggattaaagc tatgggtttt
    20161 ctttccggta agcgcattct ggtaaccggt gttgccagca aactgtccat cgcctacggt
    20221 atcgctcagg cgatgcaccg cgaaggagct gaactggcat tcacctacca gaacgacaaa
    20281 ctgaaaggcc gcgtagaaga atttgccgct caattgggtt ctgacatcgt tctgcagtgc
    20341 gatgttgcag aagatgccag catcgacacc atgttcgctg aactggggaa agtttggccg
    20401 aaatttgacg gtttcgtaca ctctattggt tttgcacctg gcgatcagct ggatggtgac
    20461 tatgttaacg ccgttacccg tgaaggcttc aaaattgccc acgacatcag ctcctacagc
    20521 ttcgttgcaa tggcaaaagc ttgccgctcc atgctgaatc cgggttctgc cctgctgacc
    20581 ctttcctacc ttggcgctga gcgcgctatc ccgaactaca acgttatggg tctggcaaaa
    20641 gcgtctctgg aagcgaacgt gcgctatatg gcgaacgcga tgggtccgga aggtgtgcgt
    20701 gttaacgcca tctctgctgg tccgatccgt actctggcgg cctccggtat caaagacttc
    20761 cgcaaaatgc tggctcattg cgaagccgtt accccgattc gccgtacagt tactattgaa
    20821 gatgtgggta actctgcggc attcctgtgc tccgatctct ctgccggtat ctccggtgaa
    20881 gtagtccacg ttgacggcgg tttcagcatc gctgcaatga acgaactcga actgaaataa
    20941 tcgttctgtt ggtaaagatg ggcggcgttc tgccgcccgt tatctctgtt atacctttct
    21001 gatatttgtt atcgccgatc cgtctttctc cccttcccgc cttgcgtcag gataacgatt
    21061 tcctttacga ccaaggagcg cccatggaac aacgccacat caccggcaaa agccactggt
    21121 atcatgaaac gcaatccagt actacggagt atgacgttct gcctctggtc ccggaagccg
    21181 caaaggtcag cgatcccttt ctgctcgacg tgatccttga tgaagagacg ctggccccct
    21241 tcctttcatg gctggtccct gcgcgcgttc ttgcagtgga gttgttccct gaccagctta
    21301 ccgtgacccg ttcacaaacc ttcaccgctt atgaacgctt gtcgacggcc ctgacggttg
    21361 ctcaggtttg cggcgtccag cggttatgta actactattc ggcgcgactt acgccgctcc
    21421 ccggacctga ttcctccagg gaaagtaatc atcggttggc acaaatcacg caatatgccc
    21481 gccaactggc tagctcgcct tctattatcg acaaccgatc gcgccagcat ctgaatgacg
    21541 tcggtcttac tgtctgggac tgtgtgatca ttaaccaaat cattggtttt attggctttc
    21601 aggcgcggac aattgcgaca tttcaggctt atctcgggca tccggtacgc tggttacccg
    21661 ggctggcgat acaaaactac gccgacgcgt cactgtttgc tgatgaatca atacgctggc
    21721 gaagcagcta tgaggtggaa aaactacctg aagagcacac aaaaagttca actgcagaac
    21781 tttgccaact ggccgaaaca ctctctctcc accctatttc actttccctt ctcgaaaagt
    21841 tgttaaacag cacacgggtc aatgcacagc cggataatca gcttgcggcg ttgttatgcg
    21901 cgcgtataaa tggcagtcct gcttgttttt ccacctgtat ggattcatca aatgaatata
    21961 aaaaaatcag cacccttctg cgcaagggcg aaaatgaaat taaccgatgg gctgaccgtc
    22021 attctgttga gcgcgctacc gttcaggcga tacaatggct gacccgagca cccgatcgct
    22081 ttagcgccgc ccagttcagc cctttactcg aacacgaaaa atcatcaacg cagattatta
    22141 atctgctggt atggagcggg ctgtgtggct ggataaatcg cttaaaaatc gcgttgggtg
    22201 agacatatta accttgccgc gtcagacaga ttcgcgtaaa actgtcagcc gctctaatgg
    22261 ccaccaaaat agacaattat gtttcaggac aacccgctgc tagcgcagct taaacagcaa
    22321 ctgcattccc agacgccacg cgctgaaggg gtggtaaaag ccacagaaaa aggctttggc
    22381 ttcctggaag tcgacgcaca aaaaagttat ttcattccgc cgccgcagat gaaaaaagtc
    22441 atgcatggcg accgaattat cgcggtgatc cacagtgaaa aagaacgtga atccgcagag
    22501 ccagaagaac tggttgaacc gttcctgact cgtttcgtgg gtaaagttca gggcaaaaat
    22561 gaccgtctga ccatcgttcc tgatcatcca cttttaaaag acgccattcc ttgccgcgca
    22621 gcccgtggcc tgaaccacga gtttaaagaa ggcgactggg cggttgccga aatgcgccgt
    22681 catccgctga aaggcgatcg ttctttctat gctgaactga cacaatacat cacttttggt
    22741 gacgaccact ttgtaccgtg gtgggttacc cttgcgcgcc ataatctgga aaaagaagca
    22801 ccagacggcg tcgctaccga aatgctcgat gaaggtctgg ttcgtgaaga tctgacctcg
    22861 ctggattttg tcaccatcga cagtgccagc actgaagata tggatgacgc ccttttcgct
    22921 aaggcgctgc cggatgacaa acttcagctg attgtggcga ttgccgatcc aaccgcgtgg
    22981 attgctgaag gcagcaagct ggacaaagcc gcgaaaattc gcgcattcac caactatctg
    23041 cctggcttca acatccctat gctgcctcgc gagctttctg acgatctctg ctcactgcgc
    23101 gccaatgaag tccgcccggt actggcatgc cgcatgacgc tctccgctga tggcaccatt
    23161 gaagataata tcgaattctt tgccgccacc atcgaatcca aagcgaagct ggtgtatgac
    23221 caggtttctg actggctgga aaataccggt gactggaagc ctgaaagtga agcaattgcc
    23281 gaacaagtcc gtttgctagc gcaaatttgc caacgccgcg gcgagtggcg tcataaccac
    23341 gcactggtgt ttaaagatcg cccggattac cgctttattc tcggtgaaaa aggtgaagtg
    23401 ctggatatcg tcgccgagcc tcgtcgcatt gccaaccgta tcgtcgaaga agcgatgatt
    23461 gccgctaaca tttgtgcggc ccgcgtactg cgcgataagc tcggttttgg catctataac
    23521 gtgcatatgg gctttgatcc ggcgaatgcc gacgcgctgg cagcgttgct gaaaacgcac
    23581 ggtctgcatg tcgatgccga agaagtgctc acgctggacg gtttctgcaa actgcgtcgt
    23641 gaactggatg cgcaaccaac tggtttcctc gacagccgca ttcgtcgctt ccagtcattt
    23701 gctgaaatta gctctgaacc cggtcctcac tttggcctcg gtctggaagc atacgccacc
    23761 tggacttcgc cgatccgtaa atatggcgac atgatcaacc accgtctgct gaaagcggtt
    23821 atcaaaggcg aaactgcgac gcgtccgcag gatgaaatca ctgtccaaat ggccgaacgt
    23881 cgccgtctca accggatggc agaacgtgat gttggcgact ggttatacgc acgcttcctg
    23941 aaagataaag ccgggaccgg cacccgtttc gcggcggaaa ttgtcgatat cagccgtggc
    24001 ggcatgcgtg tgcgtttggt tgataacggc gctatcgcct ttattccggc acctttctta
    24061 cacgctgtgc gcgatgaact ggtttgcagc caggaaaacg gcactgtaca aattaaaggt
    24121 gaaacggttt acaaagtaac tgacgttatt gacgtcacca ttgccgaagt ccgcatggaa
    24181 acccgcagca ttattgcgcg cccggtcgcg taatctcctt tcacggccca ttcctcatgg
    24241 atgggccgtt tatttccccg ctctaccttc gttatatcca ggcagatttt ttaactgact
    24301 ttcgtttgaa aactggcgtt tttccaaaac tggattacaa ataaatatat aaaaaaacaa
    24361 tgcgtttagc cgccgttaat ttcatgtaag tcatgttgtt ccttttattt aaccgcggac
    24421 tccgctgtta accggaggat atgcatcatg aaaaccgtta gggagtccac aacgttgtac
    24481 aactttctcg gatcgcacaa tccatactgg cggttgacgg aaagcagcga tgctttgcgc
    24541 ttttctacca ccaaaaccac agaacctgat cgtacattgc agttatctgc cgaacaggct
    24601 gctcgcatca gggaaatgac ggtcatcacc tccagcctga tgatgagtct gaccgtcgat
    24661 gaaagcgatc tttctgtgca tctggtagga cgaaaaatca ataaacggga atgggctggc
    24721 aacgcgtctg catggcatga cacaccggcg gttgctcgtg atttatcaca cgggctttcc
    24781 tttgctgagc aggtagtttc tgaagcacat tccgcaatag tgattctcga cagccggggg
    24841 aatatccaac gcttcaatcg gttatgtgaa gattacacag ggttgaaaga acacgacgtc
    24901 attgggtaaa gcgtgtttaa actgtttatg agccgtcgtg aagctgcggc atccaggcgc
    24961 aataaccgtg tattttttcg aagcggcaat gcatatgaag tcgaactgtg gataccaaca
    25021 cgtaaaggcc agcggctgtt tctgtttcgc aataaatttg tccacagcgg cagtggcaaa
    25081 aacgagattt ttttaatctg ttccggtacc gacattaccg aagagcgccg cactcaggag
    25141 cgactgcgta ttctggcaaa taccgacagt atcaccggac tgccgaatcg taacgcaatg
    25201 caggagttaa tcgatcacgc tattaatcag gcagataaca ataaagttgg ggttgtgtat
    25261 cttgatttgg ataattttaa aaaggtcaac gacgcctatg ggcatttgtt tggtgaccag
    25321 ttattacgcg acgtgtcatt ggctatttta agctgtctcg aacatgacca ggtgttggcg
    25381 cgtccaggtg gcgatgagtt tctggtactg gcatccaata cctcacaaag cgcgctggaa
    25441 gcaatggcat cacgaatttt gacccgctta cgcctcccct ttcgtattgg tttaattgaa
    25501 gtttatacca gctgttcagt aggtatttcg ctctctcccg aacatggttc ggacagcgcg
    25561 actattattc gtcacgccga cacagcaatg tacacagcga aggaaggcgg acgaggacaa
    25621 ttttgcgttt ttactccaga aatgaatcag cgggtatttg aatatctctg gctggatacc
    25681 aacttgcgta aagcactgga aaacgatcag ttggttatcg aaaatcacct agcgtgtcga
    25741 agtgcgcagt ctggaagcac tagtacgttg gcagtcacct gaacgtgggt tgattccacc
    25801 gttggacttc atttcctacg ccgaagagtc agggctaatt gtgcctttag gccgttgggt
    25861 gattctcgat gtcgtacgcc aggtggcaaa gtggcgggat aaaggcataa acctgcgagt
    25921 ggcggtaaat atttctgcac gtcagctcgc cgatcaaacc attttcaccg ccctgaaaca
    25981 ggttctccag gaactcaatt ttgaatactg ccctatagat gttgaactga cagagagttg
    26041 tctgattgag aatgatgaac tggcactgtc tgttattcaa caatttagcc aactaggtgc
    26101 gcaagtgcat ctggatgatt ttggtaccgg ctactcttca ctttcgcaac tggcgcgctt
    26161 tccgatcgat gccatcaaac ttgaccaggt ttttgttcga gatattcaca aacaacctgt
    26221 ctcgcagtca ctggtccggg cgatcgtcgc tgtggcccag gcattgaatc ttcaggtgat
    26281 cgccgaaggt gtagagagtg caaaggaaga tgctttttta accaagaacg ggatcaatga
    26341 gcggcaagga tttttgtttg ccaaaccgat gcccgccgtc gccttcgaac gctggtataa
    26401 acgctatctg aagcgcgcat aagtagatga atatatatac ttagcgctat atcccgtcat
    26461 tatatatcgt attcctggat gataattagc cgttgctgtt aaacacttct cttttagcgg
    26521 caatttttgc attattcatt ttcccccttt tgctataagg atgtccttga tgtctgaatt
    26581 tgatgcccag cgcgttgccg aacgtattga tattgtgtta gatatccttg tggcaggcga
    26641 ttaccactct gcgatccata atctcgagat acttaaagca gaactcttgc gccaggttgc
    26701 tgaatctacg ccagacatcc cgaagacacc gtgggagatt taacaatcgt gccttcaggt
    26761 tttcctattt gaggatgccc ggcgcgtcag tattttttta tttagtatta taacgttata
    26821 agaattacag cgatgaattc ccgacaacaa actattctac agatggtcat tgaccagggt
    26881 caggttagcg taaccgatct ggcaaaagcc actggagttt ctgaagttac cattcgccag
    26941 gatctcaaca ccctcgaaaa actgagttac ctccgccgtg cacatggctt tgcagtttcg
    27001 cttgatagtg atgacgtcga aacccgtatg atgagcaact atacgctgaa gcgtgaactc
    27061 gccgagtttg ccgcgtcact ggttcaaccg ggcgaaacca tctttatcga aaatggcagc
    27121 agcaatgccc tgctcgcccg gactctgggg gagcagaaga aaaatgtcac tatcatcacg
    27181 gtcagcagct acatcgcgca tttgctgaaa gacgcgcctt gtgaagtcat tttgctcggt
    27241 ggcgtgtacc agaaaaaaag cgaaagtatg gttggccctt tgacacgcca gtgcatccag
    27301 caggtacatt tcagcaaagc atttattggt attgatggct ggcaacctga aactggattc
    27361 accggtcgcg atatgatgcg caccgatgtg gtcaatgccg tgctggaaaa agagtgcgaa
    27421 gcgatagtcc tgactgacag ctcgaaattt ggtgctgtac attcatactc catcggtccc
    27481 gttgagcgat tcaatcgcgt gattaccgat tcgaaaatac gcgccagcga tctgatgcat
    27541 cttgagcaca gtaaactcac cgttcacgtc gttgacattt aaccatttcc cttctctacg
    27601 gatgatttgc agtttggcaa atcatccgct ctaagatgat tcctggttga taattaagac
    27661 tatttacctg ttattaacac tctcaagata taaaattatt atcagcgata taacaggaag
    27721 taattatcac ctgcgtgata taaccctgcg cgcgagcaga tttcacggaa taatttcacc
    27781 aggcttattc ttagctatta tagttataga gagcttactt ccgtgaatca taaattcagg
    27841 agagagtatt atgtttgtaa cgagcaaaaa aatgaccgcg gctgttctgg caattacttt
    27901 ggcaatgtct ctgagtgcct gttctaactg gtctaaacgg gaccgcaaca ccgcaatcgg
    27961 cgcgggtgca ggggcattag gcggtgcagt actgaccgat ggcagtacgt tgggtacatt
    28021 aggtggtgca gctgtcggtg gtgttattgg tcaccaggtc ggtaaataat cgtaaataaa
    28081 tgtcgattat cgactcgcaa ttataatacg cttgattctg ttcagtacga aataactgaa
    28141 taatacacac agtaaaatat tcgaaagcca cggatatatc cgtggctttt tatgttaacc
    28201 gcctgcgagt tttaccttca tccctttcgc ttcaagtagt gattttaata aatcacgctt
    28261 atcgccctgg atttcaataa ctccatcttt aactgctccg ccgcagccgc attttttctt
    28321 cagttccgct gcaagttttg tcagttcggc atcatcgaga tcgataccgg taatcaggca
    28381 aacgccctta cctttacgtc cactggtctg acgctgaata cgcaccgctc catcgccttt
    28441 agggcgtact ggggccgctt tgggctcatc aatacgcccg gtttccgttg agtagaccag
    28501 acggctgttg gaatcactca tcatgcactc cgctgtaaag aggcgttgat cgctttcagt
    28561 gtctgcgctg gatctaccga ttgcgttacc gggcgaccaa tcaccatata atcaacacca
    28621 gccgccaacg cctgttctgg cgtcataatg cggcgctggt caccagcttc actcccctgc
    28681 ggacgaatgc ccggtgtaac cagtttgaac tcctgaccga atacctgttt aaagcgcaca
    28741 gcttcctgag cagaacacac cacaccatca aggtcacatt tttgcgtcag tgctgccaga
    28801 cgttctgcat aatctgcagg tgacagtgtc acgccaagat cggccaggtc gctggcttcc
    28861 atgctggtca acactgtcac agcaatcaaa agcggtgcat ctttgccaaa cggaaccagt
    28921 gcctcacgcg ctgcggtcat catacgcgcc ccaccggagg catgaacatt caccatccac
    28981 acgcctaagt cagctgcagc agcgacagcg tgcgctgcag tgttggggat atcgtggaat
    29041 ttcaggtcaa gaaagatatc aaaaccacgc tgttgaagtt cgcgcacaaa ctgtggccca
    29101 aacaatgtaa acatctcttt accgaccttc agacgacaat cgcgtgggtc gatcttgtcg
    29161 acaaaggaca gcgcgtcatc acaattatga taatcaaggg caacaaccac aggagaattc
    29221 gtaacggcgc gggaagaaga tgaagcagtt aacgtcatga ccagaccttc ttgatgatgg
    29281 gcaccgggag tggcgcgaaa caggtaaacg gcgagcattc tacccgtgaa aataaaaaat
    29341 taacagttgc gatttttccc ctagcaggct ttttctgctc cgtcgttgcc ggagcacaat
    29401 gggacatcaa taactagtat gttgtaacta aagtcggatt tttttaaaaa ttacaggcca
    29461 tcaagaccgc gaatcggttt aatagttgac caggcacgac aagacggaca atgccagtag
    29521 agggtgtatg cggtaaaacc acatttctgg cagctataac gaggcttact acgcaccttc
    29581 tcgccaacca tgtcacgcag caccatcaga ctctctttgg cgcgcccttc ttccgcttca
    29641 tttaagtggt aatccattag cttatggaat acacgcatgg tcggatgacg ctgaagctgg
    29701 cgtgtaatat agacctgtgc agcctcacta ccgtcgcgcg cttcgatgat atcagcaagc
    29761 atcaattcag catcggcacc ggtgttctct tccaccgcgc gttgcaggaa ttctgcccat
    29821 tcggcagttt tacccaactg ctgatagcag gtttgcaaca tttccagcgt ttcgctgacc
    29881 agttctctgt cctgggatat gacgcgttgc aaactttcga cggctttggc gtattctcct
    29941 ttcgccataa acacgcgtcc catcatgatg gatacgcggg cgctgttttt atctgccgcc
    30001 gccccttttt ttagcaaggt catggcacga tcgagatcgt cgctggccat atgctgcagg
    30061 gctaactcac agtagaaatg ggcaatttcg acgcgctgtt tatctttacc cagcttcacc
    30121 aggcgttcgg caacatcaat tgctttctgc cactcactgg tagcctggta gatttgtagc
    30181 aactgttgca gcgcgccaat gcggaagtca gtttcatcgg tcagctgatt gaacatgtct
    30241 tccgcgcggt cgtataaccc ggcggccatg taatcacgcc ccagttgttg aatcgccaac
    30301 agacgctgtt cataggtcag cgaggcgctt tccattaggg tctgatggat gcgaatagcg
    30361 cgatcaactt cgccacgcga acggaacagg tttccgagcg taaggtgggc ttcaacggtg
    30421 cctgtatcct ctttaagcat atcgagaaac aggtctaccg ctttatcctg ttgattacta
    30481 agcaggaagt taacccccgc tacgtaatca cgcgacaagc ggttggcttc atcttgcttg
    30541 ttttgttgcg cacttctgcg gcccatatac cagccatagg cagcggctac aggcaaaagc
    30601 agaaacaaca actccagcat agaaagttat tccttcgccg ctgacgagtg cggcactaca
    30661 gccacgtcag tcgcgggtga aagctggttt tccagtcgct ttattttacg ttcagcgcgc
    30721 gccagggaaa cacgaactcg cagccagaac aggccacaaa tcaaccaacc gatagcaaac
    30781 cccgcagcaa acaataccgc cagcaatgtg gaaatacggt actccccttg cgctaacaga
    30841 taattaaacg tcacctgttg atcgttctgc gcacccaacg tgaccgaaat cacgaagatc
    30901 gctaacacca gtaagaaaat gagtaaatat ttcacattac ttcccgttat gtggttagag
    30961 cgaataaatt atggtcaact taccgcaata gccctttaaa gtaccatttt cgcgccgtct
    31021 gcgaaatggc atccacgcgc attgttgatg atttaaggcc taaattcgga aaatcaacca
    31081 gcgttaactt tcttgttctc gttgtgcaat ttcgcgattt tcttccgcag gtggtgttaa
    31141 tggcccacaa attcgttgcg caagccacgt tgccaccgcc accagcgccc acgaaatcaa
    31201 cgtagctacc accagatcgc gtggccaatg catcccgagc agcaggcggc ttcccatgac
    31261 tcccgttgcc cagaccaaca agatagcaat ggttaacgtt cgccgacgcg gccacagcaa
    31321 accaacggcc agcagtgccc aactggcagc aaacatcgtg tgaccggaag gaaaggcaaa
    31381 ccccgtctct ttctgccagt gtgaacgcaa atattgtgga atatttttct cttcagccaa
    31441 ctgttctttt actagatttc cgcgttctgc tcgctttaaa gtgtagaact catcaaccgg
    31501 aatatgatgt gttttttcca accagataac aaaaggtcgt ggttcctgta ctttgtcttt
    31561 gatccaggat ttaacgcctt gtccaacaag gattgccgcc gccagaatgg caaataatac
    31621 aaaggcagcc ttaatgcgaa aacgcagaca ccagagaaac cagccgaaca aaatcaaatg
    31681 tgtaatgacg ccccagggct gggtgacagt ttcagtaacc caaaaagccg cttttagtag
    31741 ccaactttgt tctccaggtt gccaacgcca gccagaaatc catacggcta ctggcatgac
    31801 aagcaatagt gcagctccca ctgcggtacg tcgggcaatc gaacgcatgg cctctccttt
    31861 ttgataagtc ccacaatcat aactgaaaat gccagttcca agaaaaattg acagatttgt
    31921 gccattccgt gaacgatcga cgcgtcgtga ttaggtgaac cccttctcgt tatggcaaaa
    31981 taagccaata cagaaccagc attatctgga gaatttcatg cagcttaaac gtgtggcaga
    32041 agccaaactg ccaaccccat ggggcgattt cctgatggtg ggatttgaag aactggcaac
    32101 cggacacgat catgttgcgc tagtctatgg cgatatttcc gggcataccc cggtacttgc
    32161 gcgcgtccat tccgaatgtc tgaccggtga cgccctgttc agcttgcgct gcgattgtgg
    32221 cttccagctc gaagcggcat tgacgcaaat tgccgaggaa ggccgtggta ttttgctgta
    32281 tcaccgtcag gaaggtcgta acattggtct gctgaataaa atccgcgctt acgcactgca
    32341 ggatcaaggt tacgataccg tagaggctaa ccaccagtta ggcttcgccg ctgatgagcg
    32401 cgacttcact ctttgcgctg atatgttcaa actccttggc gtcaatgaag tccgcttgtt
    32461 aaccaataac ccgaaaaaag tcgaaattct gaccgaagca gggattaata ttgttgaacg
    32521 cgtaccattg attgtaggtc gtaaccccaa taacgaacat tatctcgata ccaaagccga
    32581 gaaaatgggc catttgctga acaaataacc ctcttgcatc gtgtaattca tttgcttgcc
    32641 ggaagcaaaa taaccggcaa gcaaatactt gttacttcaa catattacga atgacataat
    32701 gcaaaatgcc gtcgttctgg tagtaggtca actccgtcgc ggtgtcgata cgacaacggc
    32761 agggtacgac ttcctggcta ccatccgcgc gcgtaagcgt caccggaacc gtcgcgccag
    32821 gttgtagatt ttgcagatcg ccaatatcaa tcttctcttc cccggttagc cctaacgttt
    32881 tacgcgtcac gccttgcgga aattccagcg gcaggatgcc catgccaatt aaattcgaac
    32941 ggtgaattcg ttcaaacgat tcggcaatca ccacacgaat tccaagcaga cgaggtcctt
    33001 ttgccgccca gtcacggctt gagcctgatc catactcttt cccggcaatc accgccagcg
    33061 gcgtttgctc ctgcttatag cgcatcgcag catcataaat agagactacg tcgctgtcag
    33121 gtaaatgccg cgtcatcccc ccttcaacgc caggcaccat ttcattacgg atgcgaatat
    33181 tggcgaaggt gccgcgcatc atcacttcat ggttaccacg ccgcgaaccg taggagttaa
    33241 agtcttttcg ctcaacaccc cgaccttgta gatatcgacc cgctgggctg tcgggcttaa
    33301 tactgcccgc cggagagata tggtcagtgg tgactgaatc ccccagcatt gcgaggatcc
    33361 gcgcaccgtg aatatcttcc actggtgctg gtgttgcctg catttcatca aagaaaggcg
    33421 ataagcgaat ataggttgag tcctcctgcc aaccgtaggt atcggatcgt gtgacgttaa
    33481 tttccttcca ctctgctgtg ccttcaaaaa cttctgcgta ctctttgcgg aacatttctg
    33541 tggagacttg ttctaccgca cgggcaattt cttgtgccga tggccagata tctttcagat
    33601 aaaccggctc gcctttgcga tcatggccga taggctcaga agccaggttg atattcatat
    33661 ttcccgccag cgcataggca accaccagcg gtggcgaggc cagccagtta gttttaacca
    33721 gcggatggat acggccttca aagttacggt tgccggacag caccgcaccg acggttaaat
    33781 cgcctttttt gattgccgtt tcgataggat cgggcagcgg cccagagtta ccaatacagg
    33841 tggtacaacc gtatcccaca aggttaaacc ccagttcgtc gagatacggt gtcagtttcg
    33901 cttttgccag ataatcagaa acgactttcg aacccggtgc caacgacgct ttgacccacg
    33961 gttgccgctt gaggccaaga gttacggctt ttttcgccag caagcctgcg gccatcagca
    34021 cacttgggtt agaggtgttg gtgcacgagg ttatcgcagc aatgaccaca gcgccatcag
    34081 gtaactgata ctgatgtccg ttcataacat aatcgactgg caggcgatct ttatgcgtgg
    34141 cattcacttt cagttcgtta ctggcggcaa atgcttttgg tacatcgggc agtgcaacgc
    34201 gatcctgtgg gcgttttggc cctgccaggc tcgcttcaac gtcattcata tccagttcta
    34261 acatactggt aaaaatcggt tcatcgcccg ggttacgcca catgccctgc gctttggcat
    34321 atttttcgac caactcgacc tgatcttcgc tgcgcccgct taaacgcatg taatcgaggg
    34381 ttactgcatc gattgggaag aagccacagg tggcaccata ttctggcgac atattggcaa
    34441 tggtggcgcg atccgccaac ggtagtgaat ccagaccatc accataaaat tcgacgaatt
    34501 tccccaccac gccatgtttg cgcagcattt gggtaacagt gagaaccagg tctgtggcgg
    34561 taataccttc acgtaatttt ccggtaagtt taaagcccac tacgtccggg ataagcatgg
    34621 aaaccggctg gcctaacatg gcggcttctg cttcgatccc acccacgccc caccccagca
    34681 cgccaaggcc gttgatcata gtggtgtgcg agtcagtacc aacgagtgta tccggataag
    34741 caatccattc accgtcctgc aattcgctcc acactgcttt gccgagatat tcgaggttaa
    34801 cctgatggca aatgcctgtg cctggcggca cgacgctaaa ccgactgaac gcttgctttc
    34861 cccatttcag gaacacataa cgttcgtggt tgcgctccat ttccaggcgt acgttttctt
    34921 caaatgcctc atcatcacca aaacgatcga cggtcaccga gtggtcaatg accaggtcga
    34981 ccggtgagag cgggttaacc tttgcagtat cgccaccgag gcgtttaacc gcttcgcgca
    35041 ttgccgccag atcaacaacg gcaggtacgc cggtaaagtc ctgcatcagc acccttgccg
    35101 ggcggtaggc aatttcacgg tcagcatggg catttttcag ccatcccgcc agtgcgtgga
    35161 tatcctcttc ggtaactgag ttaccatcct gccagcgcag caggttttcg agcaaaactt
    35221 tgagtgactt gggtagacgg gtgatatcgc ccagtgattt agcagcaagc ggcaggctgt
    35281 agtagtggta agttttatct ttggcctgca acgtgtcctt actggcttct cgtagggttg
    35341 acgacatagc tcctccttaa tgacagggtt gcgaagccct gattctcttc agggtgaata
    35401 ttaaagataa cacaaacatc gcgtaacgat ttgataacaa cccaaattga taaaagagga
    35461 gaaaccttgt ggcaaagcaa atgacaaccc cgccgcagcg gggtcaagga tcagtgagta
    35521 atgatccaga ggagttcggc ccagaagagc atcgacagag aaaacgcgcc catccaggac
    35581 caatatttca tatttgtgtt gatcataggc tacaccctta acggttatta ttttgtgttg
    35641 agataactca agctgttatt gctgcggtgt attaaaagcc ctttaattca ttaaaaatgg
    35701 gtaataagaa tgatgataat tattgtttcg gctgatcatc ttcagcaaac agttctataa
    35761 aggcttgctg ctgcgatgtt agttgccagt cgtctggaat aacgacggaa cattcccgtt
    35821 tcgtttctgg ttcattgtca ctatgctggc ataatggtga cgactccagt tgctcctgac
    35881 cgaccatagt tatccccaca ctttccaaat cagcagtgcg acaaccaccc agaaaagtgc
    35941 agaaccaaga aaaaccgcca gccaggcttt acgttttaat tcaggatcac ggcggggttc
    36001 ctgatttcca gaaggcattg ctaacctcat ataatcgcca tcacttatca gcaagacgtt
    36061 gaatgattgg tgtaattaat catacgatga gacaaatcct aaagaaactt tagtcaaaaa
    36121 tccagcgaat ttacgaatcg tctccagtga ataattcaat cttttgacct gaaatagata
    36181 tttgaaaact ggaatttgcg aggcgggtaa ttagacacta ctttacaggt aatttaccct
    36241 ataaatatga gggataaaaa tattgtgcac ggtatagaaa taagtaacaa aagagaatat
    36301 attccggcac cttcgctaca taaaaggtgc cgaaaataac gcaagaaatt atttttccgg
    36361 cagttttata tctttaaaca tgacctcaat ttcttcattg gagcgcaatg cgacagccgc
    36421 atcaacgaca tcacgcgtta aatgcggtgc aaaacgctga atgaaatcat acatataact
    36481 gcgcaaaaaa gtactacggc gaaaaccaat tttggttgta ctgtggctga agatatcgtg
    36541 agcatcaacg cgcacaaggt cgggatcggc gaccggatcc accgccatgc tggcaatgac
    36601 ccctaccccc agccctaacc ggacgtaagt tttaatgacg tcagcatccg ttgccgtgaa
    36661 aacgatacgc ggcgttaacc ctgcgcggtt aaaggcagta tccagttctg aacgtccggt
    36721 aaagccgaag gtatatgtca ccaacggata ttgcgccagt tcttcaatgg taatggcttt
    36781 tttgcctgcc agcgggtgat ccggagtgac tacaatagcc cgattccagt ggtagcacgg
    36841 taacatcact aaatcttcat acagatgcag cgcttctgtg gcgatagcga aatcggcatt
    36901 gcctttagag acggcatcag caatttgtgt cggcgggccc tggtgcatat gcaaagaaac
    36961 gcggggataa cgctcaataa agcctttgat aacgtttggt aacgcgtagc gtgcctgggt
    37021 atgcgtggtg gcgatataca gcgagccttt atccggccag gtgtgctcac cggcaaccga
    37081 ttttatggca tcgactttcg acaggacttc gcgagcgata cgaattattt cttgtcctgc
    37141 tggcgttacc tgcgtcaggt gcttgccgct tcgggaaaaa atttgaatgc ctagctcgtc
    37201 ttccagcatt ctgacttgtt tactgatccc gggttgtgat gtgtaaagtc cttccgctgt
    37261 tgatgagaca ttcaggttat gattgaccac ctcaacaata tagcgaagtt gttgtaattt
    37321 catgttaaac catccactta gacgccatca gaacatcgtt ttttcgtcac acctggcggg
    37381 tgactaatac gatttaataa taaaaaaggt gctaactata accactatat catttatagc
    37441 cttagtgtat aggtgtgaag aaaaaataat atgcaataaa aaagggccgc tttcgcgacc
    37501 ctttgtttat aaaaacctga cagaattaaa ggttattttt ttccttcaac ccatttgcca
    37561 tcaacataaa atgctgacca gccagtcgcc tttccgtctt tttccgaaga gacatattgc
    37621 tgtttggttt tacggctaaa gcgaaccatg gtcttattac cttccggatc ctgctgtggc
    37681 gcatcggcca gataacgcag tttttccggc agacggtcgc ggaagcgata aagctcttcc
    37741 accagtggcg cacgcgtttc acgcgatttc gggaaagtgt tggcagccag gaacacaccg
    37801 gcagcaccgt cacgcagcac gaaataagca tctgattttt cgcacggcag ctcaggtaat
    37861 ggcaccggat cttctttcgg tggtgccact tcaccgttac gtaaaatctt acgtgtgttt
    37921 ttacactctt cgttggtgca ggccatgtat ttaccgaaac gccccatttt caggtgcatt
    37981 tcagagccac atttttcaca ctcaacgatc gggccgtcat aacctttaat gcggaattcg
    38041 ccctcttcga tctcgtaacc gtcgcaggtt gggttattac cacagacatg caacttacgt
    38101 ttcggatcga tgagatagct gtccatcgcc gtgccgcatt tcgggcaacg acgttttgcg
    38161 cgcagcgcgt tggtttcagc atcttcgcct tccagcacgt tcagcacttc gttttccggc
    38221 accaggttaa tggtggtttt gcaacgctct ttcggcggca gcgcatagcc agaacagcca
    38281 aggaataccc cggtactcgc tgtgcgaatc cccattttgc gaccacaagt cgggcagtca
    38341 atgctggtca gaaccatctg gttcgggcgc ataccaccct cttccggatc tttttcagct
    38401 ttatctaact gctgggtgaa atccgagaag aagtgatcca gtacagcttt ccactctgct
    38461 tcgtgatttg ccacctggtc gaggctgttt tccatctgcg cggtaaagtc gtagttcatt
    38521 aactcgcgga aattttcttc aaggcgatcg gtgacgattt cgcccatttt ttccgcatag
    38581 aaacgacgat tttctactcg cacatagcca cgatcctgaa tggtcgaaat gatcgacgca
    38641 taggtagacg gacgaccgat accacgtttt tccagttctt taaccagcga tgcttcactg
    38701 aaacgggctg gcggcttggt aaagtgctgg gctggtgtta gttcaacgag cgtcagagca
    38761 tcgcctttat cgactgccgg taagatacga tcttcatcgc ctttacgcag tgcaggcatc
    38821 acttttgtcc agccatcaaa gcgcaaaata cgaccgcgtg ctttcaggcg gaaatcgccc
    38881 gcaccaacgg tcagcgtcgt ggagtcatat ttcgctgggg tcatctggca ggcaacgaac
    38941 tgacgccaga ttaactggta cagtttctgc gcatctgctt ccatatcctt cagcgattcc
    39001 gccatcacat tgacgtcaga aggacgaatc gcttcgtgcg cttcctgtga gttttctttg
    39061 ctggcgtact gattcggact ttccggcaga tatttcttac caaaattatc gctgatataa
    39121 ccgcgaacca tatttaccgc gtcctgactc aggttagtgg agtcggtacg catgtaagtg
    39181 atatagcctg cttcatacaa acgctgcgcc atcatcatgg tttttttcac gccaaaacca
    39241 agacgggtgc tggcagcttg ttgcagcgta gaggtaataa aaggagcgcc aggtttactg
    39301 gttgtcggtt tgtcttcacg ttccagcacg ctgtagcgcg ctttttccag cagacttacc
    39361 gcagcctgag tttgttcttt gttgaccgga cggaacggtt tgtcattctg atgagtcacc
    39421 tgcaactcca acgcttcacc agatggcgta gtcgtgctgg catcgacttc ccagaactct
    39481 tccggtacga acgctttaat ttcacgctca cgctctacca ccaggcggac cgccaccgac
    39541 tgcacacgac cggcagacag accacgagcg atctttttcc acagcagcgg cgaaaccata
    39601 taccccacca cgcggtccat aaagcgacgc gcctgctggg cattaacacg atcaatattc
    39661 agctcacccg gtttgttaaa tgcctggcgg atcgcgtttt tagtaatttc gttaaacacc
    39721 actcggctat agcgcgcatc atcaccacca atcacttccc gcaggtgcca tgcaatggct
    39781 tccccttcgc ggtcaaggtc ggttgcgaga tagatgtggt cggctttttc agccagttgt
    39841 ttcagttcag agacgacctt ctctttacca ggcaacactt catagtgcgc ctcccaattg
    39901 tgccacgggt caacccccat acggttgacg agagcgccac gttcatcctt tttaggcttt
    39961 ttagccgtct tggtggaggt agagtcggca ctctttttgg cagctgagcc actggtcggc
    40021 aaatcgcgga tgtgaccgac gctggatttc accacgtagt cacaacccag atacttgttg
    40081 atcgttttgg cttttgccgg ggactcaacg atgacaagag ctttacccat attcacctta
    40141 cctaatttga ttcttccagg aatgcgtcgc acgttgataa accttccact ggcaacgagt
    40201 taccgatatg gactcgctct ctgcggatat caaccccttg tcgtgactct gttcacgaaa
    40261 attcaggtga ctgagttaac aggtcttggc ctacaggcgc tatagcacga cggatttttg
    40321 gtcgaatgtc aagcaaatct attgccagat tgacgaaagc gtcacactgt acctgataaa
    40381 atccgatacg caactttatt agcatgcaaa atccaaagtt gccagtaagg cgctcttgcc
    40441 accagcgtgg tatatttgtc tgcaagccat gcctggcgta gactaatgtc cttgtttaag
    40501 gagtaaataa tgaacaaaga gacacaaccc atcgaccgcg aaacgctgtt gaaagaggcc
    40561 aacaaaatca ttcgtgagca tgaggatacg ctcgcaggga ttgaagccac cggcgtcacg
    40621 cagcgtaatg gcgtgctggt atttaccggc gactactttt tagatgaaca aggattgcca
    40681 acagccaaaa gcaccgcggt gtttaatatg ttcaaacacc tggcccatgt gctttcagaa
    40741 aaatatcatc tggttgatta aagcaaaacg cgaggtctta gcctcgcgtt tgtcttttac
    40801 atcaatggct tttgaccccg ctgccaccag cgtagcaaca atcgatcggc gctctctgcc
    40861 gcgctgccgg tgaatcggtc aatgagtcgt ttacgctgca tatagcgtac attaaccact
    40921 tcacggcctt ccatcaggct aagaataact tcatcactgg tgttgatttc atcaaccagg
    40981 cctttctcta ccgcctgttg tccgtaccag tgttcacccg ttgccacctg ttcaatatcc
    41041 agagacggac gcatacgctt cacaaaatct ttaaataact gatgcgtttc gttcagctct
    41101 tcgcggaatt tctcccgccc ttcttcggta ttttcaccca gcaaggtcag cgtgcgctta
    41161 tactgcccgg cggtgtgcag ttcgatatca atatctttgc ttttcaggaa gcggttaaag
    41221 ttgggcattt gcgccaccac cccaatggaa cccacaatag caaacggtgc ggaaacaatt
    41281 ttgtccgcca cacaggccat catgtaaccg ccgctggcag cgactttgtc taccgtaaca
    41341 gttaaaggaa tgtttttatc acgcagacgc tgcagctgcg aagccgccaa cccgtaacca
    41401 tgcaccatgc caccagggct ttccagacgg agcacaacct gatcctgcgg tttgaatgct
    41461 gcgagtaccg ccgttatctc ttcacgtagc gagttcactt catgggcgtc catgctgcct
    41521 ttaaaatcca gcacccagac gcgaggttta ctgtcagttg ccacctcgcc cagtttggct
    41581 ttcgcttttg ctgctttcgc ttcttgcttg tgctttttct tctgcgcttt gtgccactgt
    41641 ttttgctgat gtgagtccat cagcgccgcg gccagttctt ctttcatctc cttatactgt
    41701 tcgctgagat tgttgacccg taactcgcca cgctggcgtt tattacgttg agcaacattg
    41761 acaataatgg cggcaatcgc cgcaatcgct agcacaacgg taacgatttt agccaaaaac
    41821 aaaccatatt cagacaacaa ttccacgagt ccccaccttg gttaaacaac gctttggctc
    41881 acagtttaca acaggcatgt taagccgtct cgcagagatg ttattgcagc gaaagtatga
    41941 caaagtatcc attttgcggt gttatcgccc caggctgatt gaaatgccct tctgtttcag
    42001 gcataaagcc ccaaagtcat aaagtacact ggcagcgcga cagacaacgt cgttgaggag
    42061 tagccatgca ttaccagcca aaacaagatt tactcaatga tcgcattatc ctggtgacgg
    42121 gagccagcga tgggattggt cgtgaagccg cgatgacgta tgcacgctat ggtgcgacag
    42181 tgattctgtt gggccgtaat gaagaaaaat tacgtcaggt agccagccac ataaacgaag
    42241 aaactgggcg tcagccacag tggtttattc tcgatttgct gacctgcacg tccgaaaatt
    42301 gccaacaact ggcacagcgc attgtcgtta attatccgcg tctggatggt gttttgcata
    42361 atgccggatt gctcggcgat gtttgcccaa tgagcgaaca aaatccgcag gtctggcagg
    42421 acgtcatgca gatcaacgtt aatgccacct ttatgctcac ccaggcactg cttcctttat
    42481 tactcaaatc ggacgccggt tcactggtct ttacttcatc aagcgttgga cgtcagggac
    42541 gagccaactg gggtgcatat gcagcgtcga aatttgccac cgaagggatg atgcaggtac
    42601 tggccgatga atatcagcag cgcctgcgcg tcaactgcat taacccaggc ggtacgcgca
    42661 ccgcaatgcg cgcaagcgcc ttcccgaccg aagatccaca gaaacttaaa acacccgctg
    42721 atatcatgcc gctctacctc tggctgatgg gcgatgacag ccgccgtaaa accggcatga
    42781 cctttgacgc ccaaccgggc cgtaaaccag gaatttccca atgagtgatg aacgctacca
    42841 acagcgtcag cagcgagtga aagaaaaagt agatgctcgt gtggcccagg cccaggatga
    42901 acgcggtatt atcatcgtct ttaccggcaa tggaaaaggc aaaaccaccg cggcatttgg
    42961 tacggcaaca cgcgcagttg gtcacggaaa aaaagtgggc gtcgtgcagt ttattaaagg
    43021 cacctggcct aatggcgaac gcaatctgct ggagccacat ggcgttgagt ttcaggtgat
    43081 ggcaacgggc tttacctggg atacacaaaa ccgcgagtct gataccgccg cctgccgcga
    43141 agtctggcaa catgcaaagc ggatgcttgc tgattcctca ctggatatgg ttttgcttga
    43201 tgaactgacg tatatggtgg cgtatgacta tttgccactg gaagaagtgg tgcaggcgtt
    43261 aaatgaacgt ccacatcaac agacggtgat tatcacgggt cgaggctgtc atcgggatat
    43321 tcttgaactg gcggatacgg taagtgagct gcgccccatc agacatgcgt ttgatgccgg
    43381 tgttaaggcg caaataggga ttgattatta atctgtaaaa atgccggata tgacgtccgg
    43441 catactttga ttaaccgtta ttacgcccgc cagaacgacg actgccgctc acctgactgt
    43501 gacgtttcac cgcacgacga atctgattag ccttcatgcg acgacggtct ttttctacag
    43561 cgactttaga gctggtttct ggcggaagct ccaccagttc gcgcagatag ttagtctggg
    43621 cgaggtccag ctccgtccag ccaccgcgtg gcagaccttt tggcagtggg atatcaccgt
    43681 aacgaacacg gatcaggcgg ctcacctgca caccaaccgc ttcccacaga cgacgaactt
    43741 cacggttacg gccttcggtc agagtcacgt tgtaccactg gttgatccct tcgccgccgc
    43801 tgaacttgat ggttttaaaa gctgccggac catcttccag ttgcacgcca cgactcaaat
    43861 cacgcagttt cgcgtcgtca acctgaccaa atacacgcac ggcgtattca cgttcaactt
    43921 cacggcttgg atgcattaaa cggtttgcca gttcaccatc ggtggtgaac agcagcagac
    43981 cacaggtgtt aacgtccaga cgacctacgg caatccagcg tgcgccacgc agctttggca
    44041 gacggtcaaa caccgtcgga cgcccttccg gatcgttacg ggtacacaac tcaccttccg
    44101 gtttgtaata ggccagaacg cgacaaattt gttcagcgga ctcacgtacc gaaatcagat
    44161 gaccatcgat acgaattttc agcccagggg taacttcaac gcgatcgccg agtttagcaa
    44221 ttttgccatc aacactcaca cgaccggctt caataataga ttcgatttca cggcgggagc
    44281 cgtggccagc gcgggccagc actttctgta atttttcgct catagagctt ccttttggtg
    44341 tcgccttcac aggcgtcgaa caggagaatc aatggcgccg tatagcgcca ttttaaggcc
    44401 gcgtagtata cagagttacg cacttataag aaaggcttca catcacctac gccttcacgc
    44461 accacgaccg gcgtatcatc ggtaagatca ataaccgttg ttggtttctg cccgagatag
    44521 ccgccgtgaa taatcaaatc tacctgtttt tccagacggt ctttaatttc ttcgggatcg
    44581 gattcggtaa attcgctgcc tggcagcatt agcgaagtgg aaagcatcgg ttcgcccagt
    44641 gcttcaagca acgcctgcgc aatcgggtta gaaggcacac gcataccgat ggttttgcgt
    44701 ttctcctgca acagacggcg tggcacttcc ttcgtcccct tcaggatgaa ggtatagttg
    44761 cccggcgtgt tgtttttcat caaacgaaac gcaacgttat ccacgaatga ataggtcgag
    44821 agttcagaaa gatcgcgaca catcagggta aagttgtgac cgtccggcag ctggcgaata
    44881 cgacaaatac gctccatggc gtttttatct tcaattttac agccgagcgc atagccggaa
    44941 tcagttggat agacaatcac cccgccttta cgcacgatct ccaccgcctg gttgatcaga
    45001 cgttgctgcg ggttatcagg atgaatataa aaaaactggc tcataattcc ctctctgtgg
    45061 tgttctgcgg ctgttcccat agctgccaga cgccttcaac gcctgcgggc agccagagtt
    45121 tacgacccag ttcgatccac gggcatggct gatgaaaatc agatccttgt gatgcccata
    45181 aatgatgctg acgcgcaagt gtggccagct gggtgcgttc attgggcgat tgctggcact
    45241 gcgcgacttc catcgcgtcg ccgtggtgtt cggcaaaatg cgcaaccaat cttttcagcc
    45301 atttagcact aagattgtac cgcccaggat gagctagcac cgccttaccg ccagaatgat
    45361 gaatgacatc aatagcttgt tctattgtac accactgtgg cggaacgtat ccggttttcc
    45421 cgcgcgccag atacttttta aagacatctg ccattgaact ggctttgccg cactccacaa
    45481 ggaaacgcgc aaaatgaccg cgcgtcactg cgccgccctg cgccagtcgt tgtgcgcctt
    45541 ccagcgcccc aggtatttgc gctttttcaa gtctttcggc aatttgctgc gcccgctgat
    45601 tgcgccgttc tgtctgctgc gcgaggaact cacacattag cggatgagta atatcaatat
    45661 tcagcccgac aatatgaatt tcatgatttt cccagaccgt ggaaatttcc acgccgggaa
    45721 taagattcag cgccagtccc gaacgtgaaa tttcctctct tgctggcgcg attgcagctg
    45781 tggtgtcatg gtcggtgatc gccagggtgc cgacgcgcat ctcgactgca cggtgcacca
    45841 atgcttctgg cgtcaggcag ccatcggaag ctgtggtatg gctgtgcagg tcgtaaatca
    45901 ctgcataatt cgtgtcgctc aaggcgcact cccgttctgg ataatgtttt ttgcgccgac
    45961 atcataacgg gtctggcaaa tattctgaaa tgagctgttg acaattaatc atcgaaccag
    46021 ttaactagta cgcaagttca cgtaaaaagg gtatcgacaa tgaaagcaat tttcgtactg
    46081 aaaggttggt ggcgcacttc ctgaaacggg cagtgtattc accatgcgta aagcaatcag
    46141 atacccagcc cgcctaatga gcgggctttt ttttgaacaa aattagagaa taacaatgca
    46201 aacacaaaaa ccgactctcg aactgctaac ctgcgaaggc gcttatcgcg acaacccgac
    46261 tgcgcttttt caccagttgt gtgggaatcg tccggcaacg ctgctgctgg aatccgcaga
    46321 tatcgacagc aaagatgatt taaaaagcct gctgctgata gacagtgcgc tgcgcattac
    46381 agcattaggt gacaccgtca caattcaggc actttccggc aatggcgaag ccctgctggc
    46441 actactggat aacgccctgc ctgcgggtgt ggaaagtgaa caatcaccaa actgccgtgt
    46501 gctgcgcttc ccccctgtca gtccactgct ggatgaagac gcccgcttat gctccctttc
    46561 ggtttttgac gctttccgtt tattgcagaa tctgttgaat gtaccgaagg aagaacgaga
    46621 agcaatgttc ttcggcggcc tgttctctta tgaccttgtg gcgggatttg aagatttacc
    46681 gcaactgtca gcggaaaata actgccctga tttctgtttt tatctcgctg aaacgctgat
    46741 ggtgattgac catcagaaaa aaagcacccg tattcaggcc agcctgtttg ctccgaatga
    46801 agaagaaaaa caacgtctca ctgctcgcct gaacgaacta cgtcagcaac tgaccgaagc
    46861 cgcgccgccg ctgccagtgg tttccgtgcc gcatatgcgt tgtgaatgta atcagagcga
    46921 tgaagagttc ggtggcgtag tgcgtttgtt gcaaaaagcg attcgcgctg gagaaatttt
    46981 ccaggtggtg ccatctcgcc gtttctctct gccctgcccg tcaccgctgg cggcctatta
    47041 cgtgctgaaa aagagtaatc ccagcccgta catgtttttt atgcaggata atgatttcac
    47101 cctatttggc gcgtcgccgg aaagctcgct caagtatgat gccaccagcc gccagattga
    47161 gatctacccg attgccggaa cacgcccacg cggtcgtcgc gccgatggtt cactggacag
    47221 agatctcgac agccgtattg aactggaaat gcgtactgac cataaagagc tgtctgaaca
    47281 tctgatgctg gttgatctcg cccgtaatga tctggcacgc atttgcaccc ctggcagccg
    47341 ctacatcgcc gatctcacca aagttgaccg ttattcctat gtgatgcacc tcgtctctcg
    47401 cgtagtcggc gaactgcgtc acgatcttga cgccctgcac gcttatcgcg cctgtatgaa
    47461 tatggggacg ttaagcggtg cgccgaaagt acgcgctatg cagttaattg ccgaggcgga
    47521 aggtcgtcgc cgcggcagct acggcggcgc ggtaggttat ttcaccgcgc atggcgatct
    47581 cgacacctgc attgtgatcc gctcggcgct ggtggaaaac ggtatcgcca ccgtgcaagc
    47641 gggtgctggt gtagtccttg attctgttcc gcagtcggaa gccgacgaaa cccgtaacaa
    47701 agcccgcgct gtactgcgcg ctattgccac cgcgcatcat gcacaggaga ctttctgatg
    47761 gctgacattc tgctgctcga taatatcgac tcttttacgt acaacctggc agatcagttg
    47821 cgcagcaatg gtcataacgt ggtgatttac cgcaaccata ttccggcgca gaccttaatt
    47881 gaacgcctgg cgacgatgag caatccggta ctgatgcttt ctcctggccc cggtgtgccg
    47941 agcgaagccg gttgtatgcc ggaactcctc acccgcttgc gtggcaagct gcccattatt
    48001 ggcatttgcc tcggacatca ggcaattgtc gaagcttacg ggggctatgt cggtcaggcg
    48061 ggcgaaattc ttcacggtaa agcgtcgagc attgaacatg acggtcaggc gatgtttgcc
    48121 ggattaacaa acccgctgcc ggtggcgcgt tatcactcgc tggttggcag taacattccg
    48181 gccggtttaa ccatcaacgc ccattttaat ggcatggtga tggcagtacg tcacgatgcg
    48241 gatcgcgttt gtggattcca gttccatccg gaatccattc tcaccaccca gggcgctcgc
    48301 ctgctggaac aaacgctggc ctgggcgcag cagaaactag agccagccaa cacgctgcaa
    48361 ccgattctgg aaaaactgta tcaggcgcag acgcttagcc aacaagaaag ccaccagctg
    48421 ttttcagcgg tggtgcgtgg cgagctgaag ccggaacaac tggcggcggc gctggtgagc
    48481 atgaaaattc gcggtgagca cccgaacgag atcgccgggg cagcaaccgc gctactggaa
    48541 aacgcagcgc cgttcccgcg cccggattat ctgtttgctg atatcgtcgg tactggcggt
    48601 gacggcagca acagtatcaa tatttctacc gccagtgcgt ttgtcgccgc ggcctgtggg
    48661 ctgaaagtgg cgaaacacgg taaccgcagc gtctccagta aatccggctc gtcggatctg
    48721 ctggcggcat tcggtattaa tcttgatatg aacgccaata aatcgcgcca ggcgctggac
    48781 gagttaggcg tatgtttcct ctttgcgccg aaatatcaca ccggattccg ccacgcgatg
    48841 ccggttcgcc agcaactgaa aacccgcacc ctgttcaatg tgctggggcc attgattaac
    48901 ccggcgcatc cgccgctggc gttaattggt gtttatagtc cggaactggt gctgccgatt
    48961 gccgaaacct tgcgcgtgct ggggtatcaa cgcgcggcgg tggtgcacag cggcgggatg
    49021 gatgaagttt cattacacgc gccgacaatc gttgccgagc tgcatgacgg caaaattaag
    49081 agctatcaat tgaccgctga agattttggc ctgactccct accaccagga gcaactggca
    49141 ggcggaacac ctgaagaaaa ccgtgacatt ttaacacgct tgttacaagg taaaggcgac
    49201 gccgcccatg aagcagccgt cgctgcgaac gtcgccatgt taatgcgcct gcatggccat
    49261 gaagatctgc aagccaatgc gcaaaccgtt cttgaggtac tgcgcagtgg ttccgcttac
    49321 gacagagtta ccgcgctggc ggcacgaggg taaatgatgc aaaccgtttt agcgaaaatc
    49381 gtcgcagaca aggcgatttg ggtagaaacc cgcaaacagc agcaaccgct ggccagtttt
    49441 cagaatgagg ttcagccgag cacacgacat ttttatgatg cgctacaggg tgcacgcacg
    49501 gcgtttattc tggagtgtaa aaaagcgtcg ccgtcaaaag gcgtgatccg tgatgatttc
    49561 gatccggcac gcattgccgc catttataaa cattacgctt cggcaatttc ggtgctgact
    49621 gatgagaaat attttcaggg gagctttgat ttcctcctca tcgtcagcca aatcgccccg
    49681 cagccgattt tatgtaaaga cttcattatc gacccttacc agatctatct ggcgcgctat
    49741 taccaggccg atgcctgctt attaatgctt tcagtactgg atgacgacca atatcgccag
    49801 cttgcagccg tcgctcacag tctggagatg ggggtgctga ccgaagtcag taatgaagag
    49861 gaactggagc gcgccattgc attaggggca aaggtcgttg gcatcaacaa ccgcgatctg
    49921 cgtgatttgt cgattgatct caaccgtacc cgcgagcttg cgccgaaact ggggcacaac
    49981 gtaacggtaa tcagcgaatc cggcatcaat acttacgctc aggtgcgcga gttaagccac
    50041 ttcgctaacg gttttctgat tggttcggcg ttgatggccc atgacgattt gcacgccgcc
    50101 gtgcgccggg tgttgctggg tgagaataaa gtatgtggcc tgacgcgtgg gcaagatgct
    50161 aaagcagctt atgacgcggg cgcgatttac ggtgggttga tttttgttgc gacatcaccg
    50221 cgttgcgtca acgttgaaca ggcgcaggaa gtgatggctg cggcaccgtt gcagtatgtt
    50281 ggcgtgttcc gcaatcacga tattgccgat gtggtggaca aagctaaggt gttatcgctg
    50341 gcggcagtgc aactgcatgg caatgaagat cagctgtata tcgatacgct gcgtgaagct
    50401 ctgccagcac atgttgccat ctggaaagca ttaagcgtcg gtgaaaccct gcccgcccgc
    50461 gagtttcagc acgttgataa atatgtttta gacaacggcc agggtggaag cgggcaacgt
    50521 tttgactggt cactattaaa tggtcaatcg cttggcaacg ttctgctggc ggggggctta
    50581 ggcgcagata actgcgtgga agcggcacaa accggctgcg ccggacttga ttttaattct
    50641 gctgtagagt cgcaaccggg catcaaagac gcacgtcttt tggcctcggt tttccagacg
    50701 ctgcgcgcat attaaggaaa ggaacaatga caacattact taacccctat tttggtgagt
    50761 ttggcggcat gtacgtgcca caaatcctga tgcctgctct gcgccagctg gaagaagctt
    50821 ttgtcagcgc gcaaaaagat cctgaatttc aagcccagtt caacgacctg ctgaaaaact
    50881 atgccgggcg tccaaccgca ctgaccaaat gccagaacat tacagccggg acgaacacca
    50941 cgctgtatct caagcgtgaa gatttgctgc acggcggcgc acataaaacc aaccaggtgc
    51001 tggggcaggc gttgctggcg aagcggatgg gtaaaaccga aatcatcgcc gaaaccgggg
    51061 ccggtcagca tggcgtggcg tcggcccttg ccagcgccct gctcggcctg aaatgccgta
    51121 tttatatggg tgccaaagac gttgaacgcc agtcgcctaa cgtttttcgt atgcgcttaa
    51181 tgggtgcgga agtgatcccg gtacatagcg gttccgccac gctgaaagat gcctgtaatg
    51241 aggcgctacg cgactggtcc ggcagttatg aaaccgcgca ctatatgctg ggcaccgcgg
    51301 ctggcccgca tccttatccg accattgtcc gtgagtttca gcggatgatt ggcgaagaaa
    51361 ccaaagcgca gattctggaa agagaaggtc gcctgccgga tgccgttatc gcctgtgttg
    51421 gcggcggttc gaatgccatc ggcatgtttg ctgatttcat caatgaaacc aacgtcggcc
    51481 tgattggtgt ggagcctggt ggtcacggta tcgaaactgg cgagcacggc gcaccgctaa
    51541 aacatggtcg cgtgggcatt tatttcggta tgaaagcgcc gatgatgcaa accgaagacg
    51601 ggcagattga agaatcttac tccatctccg ccggactgga tttcccgtct gtcggtccac
    51661 aacacgcgta tcttaacagc actggacgcg ctgattacgt gtctattacc gatgatgaag
    51721 cccttgaagc cttcaaaacg ctgtgcctgc acgaagggat catcccggcg ctggaatcct
    51781 cccacgccct ggcccatgcg ctgaaaatga tgcgcgaaaa cccggataaa gagcagctac
    51841 tggtggttaa cctttccggt cgcggcgata aagacatctt caccgttcac gatattttga
    51901 aagcacgagg ggaaatctga tggaacgcta cgaatctctg tttgcccagt tgaaggagcg
    51961 caaagaaggc gcattcgttc ctttcgtcac gctcggtgat ccgggcattg agcagtcatt
    52021 gaaaattatc gatacgctaa ttgaagccgg tgctgacgcg ctggagttag gtatcccctt
    52081 ctccgaccca ctggcggatg gcccgaccat tcaaaacgcc actctgcgcg cctttgcggc
    52141 aggtgtgact ccggcacaat gttttgaaat gctggcactg attcgccaga aacacccgac
    52201 cattcccatt ggcctgttga tgtatgccaa tctggtgttt aacaaaggca ttgatgagtt
    52261 ttatgcccag tgcgagaaag tcggcgtcga ttcggtgctg gttgccgatg tgccagttga
    52321 agagtccgcg cccttccgcc aggccgcgtt gcgccataat gtcgcaccta tcttcatctg
    52381 cccgccaaat gccgatgacg acctgctgcg ccagatagcc tcttacggtc gtggttacac
    52441 atatttgctg tcacgagcgg gcgtgaccgg cgcagaaaac cgcgccgcgt tacccctcaa
    52501 tcatctggtt gcgaagctga aagagtacaa cgctgcacct ccattgcagg ggtttggtat
    52561 ttccgccccg gatcaggtaa aagcagcgat tgatgcagga gctgcgggcg cgatttctgg
    52621 ttcggccatt gttaaaatca tcgagcaaca tattaatgag ccagagaaaa tgctggcggc
    52681 actgaaagtt tttgtacaac cgatgaaagc ggcgacgcgc agttaatccc acagccgcca
    52741 gttccgctgg cggcatttta actttcttta atgaagccgg aaaaatccta aattcattta
    52801 atatttatct ttttaccgtt tcgcttaccc cggtcgaacg tcaacttacg tcatttttcc
    52861 gcccaacagt aatataatca aacaaattaa tcccgcaaca taacaccagt aaaatcaata
    52921 attttctcta agtcacatat tcctcaggta attgttaata tatccagaat gttcctcaaa
    52981 atatatttcc ctctatcttc tcgttgcgct taatttgact aattctcatt agtgactaat
    53041 tttaatgagt gtcgacacac aacactcata ttaatgaaac aatgcaacgc aacgggagaa
    53101 ataacatggc cgaacatcgt ggtagttcag gaaatttcgc cgaagaccgt gagaaggcat
    53161 ccgacgcagg ccgtaaaggc ggtcagcata gcggcggtaa ttttaaaaat gatccgcaac
    53221 gcgcatctga agcgggtaaa aaaggcggtc aacaaagcgg tggtaataaa tcaggcaaat
    53281 cctgattcac cgttaattat taatatcagc tgataaagat aattgcatct gcgggccgta
    53341 tggctcgcag tgatttcact ggagaaaaat atgaatatga agaccattga agatgtattt
    53401 attcaactgc tttcagatac ctacagcgca gaaaaacaat taacccgggc actggcaaaa
    53461 ctcgcaagag caacatcaaa tgaaaaatta agtcaggctt ttcatgcgca cctcgaggaa
    53521 actcatggac agattgaacg aattgatcaa gttgtggaat cagaatcaaa tctgaaaatt
    53581 aagcgcatga aatgtgtggc aatggaaggt cttattgaag aagctaatga ggtcatcgaa
    53641 agtaccgaga aaaacgaagt gcgtgatgcc gcactgattg ccgcagcaca gaaagtcgag
    53701 cattatgaga ttgccagtta cgggacatta gcgacgctgg ctgaacaatt aggttaccgt
    53761 aaagcagcga agcttctgaa agaaaccctg gaagaagaaa aggccaccga catcaaactg
    53821 actgatctgg cccttaataa cgtaaataag aaagccgaaa ataaagcctg aaatatgaat
    53881 tttaactttt agtcatttta taaagaggac attttcatga atcgtattga acattatcat
    53941 gactggttac gtgacgccca cgcaatggaa aagcaagccg aatctatgct tgaatccatg
    54001 gccagccgta tagataatta tcctgaacta cgcgctcgta ttgaacaaca tcttagtgaa
    54061 accaaaaacc agattgttca actggaaact attcttgatc gtaatgacat ttcacgttca
    54121 gtcattaaag attccatgag taaaatggct gcgcttgggc agtcaatcgg tggtatattc
    54181 ccttctgatg aaatagttaa aggctctatt ggcggatatg tcttcgagca atttgaaatc
    54241 acctgttaca cctcactatt agcagcagca aaaaatgccg gtgatacagt ctcaattccg
    54301 atcatcgaag cgattttaaa tgaggaaaag caaatggccg actggctgat tcagcatatt
    54361 ccgcaaacaa ctgagaaatt tttaattcgt tctgaaactg atggcgtaga agcgaagaaa
    54421 taataccctt tataccatgt ccttattgac cccgtatatt acggggtcgt ttttgtgcgg
    54481 aattaaaaac gatatcctgc tgagaacata aacacccacg gatcgaggcg tacgctatcg
    54541 tgttgctgtg caccgcctag cttataattg gcggtggtat cgatatccat gtaccacact
    54601 gacatgttaa ccaaccagtc acggttaatc agataatcaa cccccacctg cccggcagct
    54661 ccccaggaat ctttcagact gagatcggaa agccccgcct ctttgccatg atcgttaaat
    54721 ccattatcaa agaaggtggt gtagttaata ccggccccaa cgtaaggacg gaatttgctg
    54781 ctggcatcac caaaatacca ctgcgccatc agtgttggcg gcagatggtg aacggttgca
    54841 atatcgccgg tcgcccgggt gccgatttta tggcggaacg gcgtcgctgc cagtaattcc
    54901 acaccaatgt tgtcggtcgc catataagta aacgtcaagc ccagttgagt gttattggtc
    54961 acgctgaatc cacccagact tcctaacgta ccaccagcac cttctgttgg acgtacggtt
    55021 gcagaacctg cacgcataaa aaattcgcct gcttcatgcg caaaggcact gccagaaaga
    55081 agagttgtta ctgccaaagc cgccactgtt aactttttca tatccgctcc gtcgttatgg
    55141 ttataaaaac gtgacgaata tacctacatt ggagtaacaa atgatcctac atagatcaca
    55201 ttacatatga taattcacca tttattgatc tggattaatt tagaagttgc attgagaatc
    55261 ctggttaatg tgatttaaat caatttttaa atattttctc tacgcaaata ttaactcgat
    55321 cgccacttgc cgcgctttgc ccacgcctga aggccagcaa ttacctccgc tttacaaaga
    55381 tatgcttttc catacaagac ctgatgctgc cacccgaggc ttaaccaggg tacaattgcc
    55441 cgctaattaa cacctgcata aaactcaagg agagtgcatg tctatcacgg cgcagtccgt
    55501 ataccgtgac accggaaatt tcttccgtaa tcaatttatg accattctgt tggtatcgtt
    55561 gctatgtgcg tttatcacag tggtgttagg gcatgttttc tcacccagtg atgcacagct
    55621 tgcgcagctc aatgacggcg tgcccgttag cggcagtagt gggttgttcg acctggttca
    55681 gaatatgtca ccggaacagc aacaaatttt gctgcaggct tcagcggcgt ccactttttc
    55741 agaattaatc ggtaacgcca ttctcgccgg aggcgtaata ttaattatcc agctggtgtc
    55801 tgcgggtcag agagtcagtg cgctgcgagc tattggtgcc agtgcgccga tattgcccaa
    55861 gttatttatt ctgatttttc tgactaccct tttagtacag attggcatca tgctggtggt
    55921 cgttccggga attatcatgg ccattttact ggccctggca ccggtgatgc tggtacagga
    55981 caaaatgggc atttttgcct cgatgcgtag cagtatgcgg ctgacttggg cgaatatgcg
    56041 tctggtggca cccgcagtac tgagctggtt gctggcaaaa acactgttgc tgctttttgc
    56101 ctcttctttt gccgcattaa ccccggaaat tggtgccgta ctggcgaaca ccttgagcaa
    56161 cctgatttct gccatattgc tcatctatct gttccgcctg tatatgttga ttcgccaata
    56221 accttaagca tttgattacg gaatcgtaaa atgaagcagt ttcttgattt tttaccgctg
    56281 gttgtctttt tcgcgtttta caagatttat gacatctatg cggctactgc agcgctgatc
    56341 gtcgccacgg cgattgtgct tatctatagc tgggttcgct ttcgtaaggt tgagaagatg
    56401 gccctgatca cttttgttct ggtggtcgtc ttcggtggct tgacgctgtt cttccacaat
    56461 gatgagttta ttaaatggaa ggttacagtc atttatgctc tgtttgcggg agccctgtta
    56521 gtcagccaat gggtgatgaa aaagccgcta attcagcgga tgctgagtaa agaactcacg
    56581 ctgccgcaac cggtatggtc gaagctaaat ctggcctggg ctgttttctt tatcctttgc
    56641 ggtctggcaa acatctacat cgcattctgg ctgccgcaaa atatttgggt caactttaaa
    56701 gtctttggcc tgaccgccct taccttaatc ttcacattgt taagcggtat ctatatctac
    56761 cgccacatgc cgcaggaaga taaatcctaa ccagactgcc agtccttcgg ggctggcgct
    56821 gtatctcctc tgttctcctc aggaaaatca tagtagcatc gcgcctgtga ttttcctttt
    56881 aagtcggttt taccatgtct acaacacata acgtccctca gggcgatctt gttttacgta
    56941 ctttagccat gcccgccgat accaatgcca atggtgacat ctttggtggt tggttaatgt
    57001 cacaaatgga tattggcggc gctattctgg caaaagaaat tgcccacggt cgcgtagtga
    57061 ctgtgcgggt tgaaggaatg actttcttac ggccggttgc ggtcggcgat gtggtgtgct
    57121 gctatgcacg ctgtgtccag aaagggacga catcggtcag cattaatatt gaagtgtggg
    57181 tgaaaaaagt agcgtctgaa ccaatcgggc aacgctataa agcgacagaa gcattattta
    57241 agtatgtagc ggttgatcct gaaggaaaac ctcgcgcctt acctgttgag taatagtcaa
    57301 aagcctccgg tcggaggctt ttgactttct gcttactgaa tttcggtggt gccgttaatt
    57361 ttaaacagga tattcaccac aatcccactg cctggcttac ccggctcata acgccatctg
    57421 cgcatcgcat ttttcacctc acgctcaaac atgttcgcag gcttggctga gaggatttgt
    57481 acgttatcca cgcgaccatc cggcgtgacg tcaaatttaa ctttaacctg cccttcaatg
    57541 cgcaatgcct gtgctcgtgc cggatactgc ggctgattac ggcttaatgc gcgtggtcct
    57601 gaagccacac tggtaaccgg cttgctggtt gcagccgttg ccgtacttga tgtggggcgc
    57661 gcgggtgccg tattttcaaa cggtgatgcc ggacgcgact ctacgggttt gacatcgcgt
    57721 tttggctgct cctgtacctt tttcaccggc ttcggttttg gcttaggttt cggcttcggc
    57781 ttttcaatga ccaccggtgc ttcttttggc ggttcgggga tcggctcagg ttccggttct
    57841 ggctctacca ccggctccgg tggcggctga acggcttgtg gcggttcgag atcagcaggc
    57901 gcaaccatcg tgacagaaat tggttgcgca ggcgcaggta gttcaataac ctgatgtacc
    57961 gaggtataga gcagccccgc cacaacagca ccatgaatgc agaccgaaag taacgtcggc
    58021 caggggaagc ggcgaggtaa atcaagggtt attgaagtca taatcattt
//
LOCUS       DAJTRC010000014        58035 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.014, whole genome shotgun sequence.
ACCESSION   DAJTRC010000014 DAJTRC010000000
VERSION     DAJTRC010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 58035)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 58035)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..58035
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.014"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(26..280)
                     /locus_tag="OO357_000942"
     CDS             complement(26..280)
                     /locus_tag="OO357_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkgJ family cysteine cluster protein"
                     /protein_id="HCR5885977.1"
                     /translation="MECRPGCGACCTAPSISSPIPGMPDGKPANTPCIQLDEQQRCKI
                     FTSPLRPKVCAGLQASAEMCGNSRQQAMTWLIDLEMLTAP"
     gene            435..1007
                     /gene="yeiP"
                     /locus_tag="OO357_000943"
     CDS             435..1007
                     /gene="yeiP"
                     /locus_tag="OO357_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007750416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P-like protein YeiP"
                     /protein_id="HCR5885978.1"
                     /translation="MPRANEIKKGMVLNYNGKLLLAKDIDIQSPTARGAATLYKMRFS
                     DVRTGLKVEERFKGDDIVDTVTLTRRYVDFSYVDGNEYVFMDKEDYTPYTFTKDQIEE
                     ELLFMPEGGMPDMQVLTWDGQLLALELPQTVDLEIVETAPGIKGASASARNKPATLST
                     GLVIQVPEYLSPGEKIRIHIEERRYMGRAD"
     gene            1230..2696
                     /locus_tag="OO357_000944"
     CDS             1230..2696
                     /locus_tag="OO357_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708071.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5885979.1"
                     /translation="MKTIASVTLPHHVHAPRYDRQQLQSRIVHFGFGAFHRAHQALLT
                     DRVLNAQGGDWGICEISLFSGDQLMSQLRAQNHLYTVLEKGADGNQVIIVGAVHECLN
                     AKLDSLAAIIEKFCEPQVAIVSLTITEKGYCIDPATGALDTSNPRIIHDLQTPEEPHS
                     APGILVEALKRRRERSLTPFTVLSCDNIPDNGHVVKNAVLRMAEKRSPELAGWIKEHV
                     SFPGTMVDRIVPAATDESLAEISQHLGVNDPCAISCEPFIQWVVEDNFIAGRPAWEVA
                     GVQMVNDVLPWEEMKLRMLNGSHSFLAYLGYLSGFAYISDCMQDRVFRYAARTLMLDE
                     QAPTLQIKDVDLTQYADKLIARFANPSLKHKTWQIAMDGSQKLPQRMLAGIRIHLGRE
                     TDWSLLALGVAGWMRYVSGVDDAGNAIDVRDPLSDKIRELVAGSSSEQRVTALLSLRE
                     VFGDDLPDNPHFVQAIEQAWQQIVQFGAHQALLNTLKI"
     gene            2814..3800
                     /gene="yeiR"
                     /locus_tag="OO357_000945"
     CDS             2814..3800
                     /gene="yeiR"
                     /locus_tag="OO357_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding GTPase YeiR"
                     /protein_id="HCR5885980.1"
                     /translation="MTRTNLITGFLGSGKTTSILHLLAHKDPNEKWAVLVNEFGEVGI
                     DGALLADSGALLKEIPGGCMCCVNGLPMQVGLNTLLRQGKPDRLLIEPTGLGHPKQIL
                     DLLTAPVYEPWIDLRATLCILDPRLLLDEKSASNENFRDQLAAADIIVANKSDRATPE
                     SEQALQRWWQQNGGDRQLIHSEHGKVDGHLLDLPRRNLAELPASAAHSHQHVVKKGLA
                     ALSLPEHQRWRRSLNSGQGYQACGWIFDADTVFDTIGILEWARLAPVERVKGVLRIPE
                     GLVRINRQGDDLHIETQNVAPPDSRIELISSSEADWNALQSALLKLRLATTA"
     gene            3839..4552
                     /gene="lpxT"
                     /locus_tag="OO357_000946"
     CDS             3839..4552
                     /gene="lpxT"
                     /locus_tag="OO357_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kdo(2)-lipid A phosphotransferase"
                     /protein_id="HCR5885981.1"
                     /translation="MIKNLPQIVLLNIVGPALFLSWYIPVNHGFWLPIDADIFYFFNQ
                     KLVESKAFLWLVALTNNRAFDGCSLLAMGMLMLSFWLKENAPGRRRIVIMGLVMLLTA
                     VVLNQLGQALIPVKRASPTLTFTDINRVSELLSVPTKDASRDSFPGDHGMMLLIFSAF
                     MWRYFGKVAGLIALIIFMVFAFPRVMIGAHWFTDIIVGSMTVILIGLPWVLLTPLSDR
                     LITFFDKSLPGKNKHFQNK"
     gene            4964..5530
                     /gene="mepS"
                     /locus_tag="OO357_000947"
     CDS             4964..5530
                     /gene="mepS"
                     /locus_tag="OO357_000947"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional murein DD-endopeptidase/murein
                     LD-carboxypeptidase"
                     /protein_id="HCR5885982.1"
                     /translation="MVKSQPILRYILRGIPAIAVAVLLSACSANNTAKNMHPETRAVG
                     SETSSLQASQDEFENLVRNVDVKSRIMDQYADWKGVRYRLGGSTKKGIDCSGFVQRTF
                     REQFGLELPRSTYEQQEMGKSVSRSNLRTGDLVLFRAGSTGRHVGIYIGNNQFVHAST
                     SSGVIISSMNEPYWKKRYNEARRVLSRS"
     gene            5711..7267
                     /locus_tag="OO357_000948"
     CDS             5711..7267
                     /locus_tag="OO357_000948"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic di-GMP phosphodiesterase"
                     /protein_id="HCR5885983.1"
                     /translation="MFIRAPNSGRKLLLTCIVAGVMIAILVSCLQFLVAWHKHEVKYD
                     TLIIDVQKYLDTYFADLKSTTDRLQPLTLDTCQQANPELTARAAFSMNVRTFVLVKDK
                     KTFCSSATGEMDIPLNELIPALDINKNVDMAILPGTPMVPNKPAIVIWYRNPLLKNSG
                     VFAALNLNLTPSLFYSSRQEDYDGVALIIGNTALSTFSSRLMNVNELTDMPVHETKIA
                     GIPLTVRLYADDWTWNDVWYAFLLGGMSGTVVGLLCYYLMSVRMRPDREIMTAIKREQ
                     FYVAYQPVVDTQALRVTGLEVLLRWRHPVAGEIPPDAFINFAESQKMIVPLTQHLFEL
                     IARDAAELEKVLPVGVKFGINIAPDHLHSESFKADIQKLLTSLPAHHFQIVLEITERD
                     MLKEQEATQLFAWLHSVGVEIAIDDFGTGHSALIYLERFTLDYLKIDRGFINAIGTET
                     ITSPVLDAVLTLAKRLNMLTVTEGVETPEQARWLSERGVNFMQGYWISHPLPLDDFVR
                     WLKKPYTPQW"
     gene            7349..9163
                     /locus_tag="OO357_000949"
     CDS             7349..9163
                     /locus_tag="OO357_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5885984.1"
                     /translation="MIVRILLLFIALFTFGAQAQAIKESYTFAVLGEPRYAFNFNHFD
                     YVNPAAPKGGQITLSALGTFDNFNRYALRGNPGARTEQLYDTLFTTSDDEPGSYYPLI
                     AESARYADDYSWVEVAINPRARFHDGSPITARDVEFTFQKFLTEGVPQFRLVYKGTTV
                     KAIAPLTVRIELAKPGKEDMLSLFSLPVFPEKYWKDHKLSDPLATPPLASGPYHVTSW
                     KMGQNIVYSRVKDYWAANLPVNRGRWNFDTIRYDYYLDDNVAFEAFKAGAFDLRMEND
                     AKNWATRYTGKNFDKKYIIKDEQKNESAQDTRWLAFNIQRAVFSDRRVREAITLAFDF
                     EWMNKALFYNAWSRTNSYFQNTEYAARNYPDAAELVLLAPMKKDLPPEVFTQIYQPPV
                     SKGDGYDRDNLLKADKLLNEAGWVLKGQQRVNATTGQPLSFELLLPASSNSQWVLPFQ
                     HSLQRLGINMDIRKVDNSQITNRMRNRDYDMMPRLWRAMPWPSSDLQISWSSEYINST
                     YNAPGVQSPVIDSLINQIIAAQGNKEKLLPLGRALDRVLTWNYYMLPMWYMAEDRLAW
                     WDKFSQPAVRPVYSLGIDTWWYDVNKAAKLPSARQQGE"
     gene            9164..10258
                     /locus_tag="OO357_000950"
     CDS             9164..10258
                     /locus_tag="OO357_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter permease YejB"
                     /protein_id="HCR5885985.1"
                     /translation="MGAYLIRRLLLVIPTLWAIITINFFIVQIAPGGPVDQAIAAIEF
                     GNAGVLPGAGGEGVRASHAQTGVGNISDSNYRGGRGLDPEVIAEITHRYGFDKPIHER
                     YFKMLWDYIRFDFGDSLFRSASVLTLIKDSLPVSITLGLWSTLIIYLVSIPLGIRKAV
                     YNGSRFDVWSSAFIIIGYAIPAFLFAILLIVFFAGGSYFDLFPLRGLVSANFDSLPWY
                     QKITDYLWHITLPVLATVIGGFAALTMLTKNSFLDEVRKQYVVTARAKGVSEKNILWK
                     HMFRNAMLLVIAGFPATFISMFFTGSLLIEVMFSLNGLGLLGYEATVSRDYPVMFGTL
                     YIFTLIGLLLNIVSDISYTLVDPRIDFEGR"
     gene            10258..11283
                     /locus_tag="OO357_000951"
     CDS             10258..11283
                     /locus_tag="OO357_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5885986.1"
                     /translation="MSRLSPVNQARWARFRHNRRGYWSLWIFLVLFGLSLCSELIAND
                     KPLLVRYDGSWYFPLLKNYSESDFGGPLASQADYQDPWLKQRLENNGWVLWAPIRFGA
                     TSINFATDKPFPSPPSRQNWLGTDANGGDVLARILYGTRISVLFGLMLTLCSSVMGVL
                     AGALQGYYGGKVDLWGQRFIEVWSGMPTLFLIILLSSVVQPNFWWLLAITVLFGWMSL
                     VGVVRAEFLRTRNFDYIRAAQALGVSDRSIILRHMLPNAMVATLTFLPFILCSSITTL
                     TSLDFLGFGLPLGSPSLGELLLQGKNNLQAPWLGITAFLSVAILLSLLIFIGEAVRDA
                     FDPNKAV"
     gene            11285..12874
                     /gene="yejF"
                     /locus_tag="OO357_000952"
     CDS             11285..12874
                     /gene="yejF"
                     /locus_tag="OO357_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708079.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin C ABC transporter ATP-binding protein
                     YejF"
                     /protein_id="HCR5885987.1"
                     /translation="MTQTLLAIENLSVGFRHQQTVRTVVNDVSLQIEAGETLALVGES
                     GSGKSVTALSILRLLPSPPVEYLSGDIRFHGESLLHASDQTLRGVRGNKIAMIFQEPM
                     VSLNPLHTLEKQLYEVLSLHRGMRREAARGEILNCLDRVGIRQAAKRLTDYPHQLSGG
                     ERQRVMIAMALLTRPELLIADEPTTALDVSVQAQILQLLRELQGELNMGMLFITHNLS
                     IVRKLAHRVAVMQNGRCVEQNNAATLFASPTHPYTQKLLNSEPSGDPVPLPEPASTLL
                     DVEQLQVAFPIRKGILKRIVDHNVVVKNISFTLRAGETLGLVGESGSGKSTTGLALLR
                     LITSQGSIVFDGQPLQDLNRRQLLPTRHRIQVVFQDPNSSLNPRLNVLQIIEEGLRVH
                     QPTLSAAQREQQVIAVMHEVGLDPETRHRYPAEFSGGQRQRIAIARALILKPSLIILD
                     EPTSSLDKTVQAQILTLLKSLQQKHQLAYLFISHDLHVVRALCHQVIVLRQGEVVEQG
                     PCARVFATPQQEYTRQLLALS"
     gene            complement(12878..13222)
                     /locus_tag="OO357_000953"
     CDS             complement(12878..13222)
                     /locus_tag="OO357_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5885988.1"
                     /translation="MTSLQLSIVHRLPQNYRWSAGFAGSKVEPIPQNGPCGDNSLVAL
                     KLLSPDGDNAWSVMYKLSQALSDIEVPCSVLECEGEPCLFVNRQDEFAATCRLKNFGV
                     AIAEPFSNYNPF"
     gene            complement(13555..14745)
                     /gene="bcr"
                     /locus_tag="OO357_000954"
     CDS             complement(13555..14745)
                     /gene="bcr"
                     /locus_tag="OO357_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter Bcr"
                     /protein_id="HCR5885989.1"
                     /translation="MTTRQHSSFAIVFILGLLAMLMPLSIDMYLPALPVISAQFGVSA
                     GSTQMTLSTYILGFALGQLIYGPMADSFGRKPVVLGGTLVFAAAAVACALAQTIDQLI
                     VMRFFHGLAAAAASVVINALMRDIYPKEEFSRMMSFVMLVTTIALLMAPIVGGWVLVW
                     LSWHYIFWILALAAILASAMIFFLIKETLPPERRQPFHIRTTIGNFAALFRHKRVLSY
                     MLASGFSFAGMFSFLSAGPFVYIEINHVAPENFGYYFALNIVFLFVMTIFNSRFVRRI
                     GALNMFRSGLWIQFIMAAWMVISAPLGLGFWSLVVGVAAFVGCVSMVSSNAMAVILDE
                     FPHMAGTASSLAGTFRFGIGAIVGALLSLATFNSAWPMIWSIAFCATSSILFCLYASR
                     PKKR"
     gene            complement(14773..15468)
                     /gene="rsuA"
                     /locus_tag="OO357_000955"
     CDS             complement(14773..15468)
                     /gene="rsuA"
                     /locus_tag="OO357_000955"
                     /EC_number="5.4.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA pseudouridine(516) synthase RsuA"
                     /protein_id="HCR5885990.1"
                     /translation="MRLDKFIAQQLGVSRAIAGREIRGNRVTVDGEIVRNAAFKLLPE
                     HDVAYDGNPLAQQHGPRYFMLNKPQGYVCSTDDPDHPTVLYFLDEPVAWKLHAAGRLD
                     IDTTGLVLMTDDGQWSHRITSPRHHCEKTYLVTLESPVADDTAEQFAKGVQLHNEKDL
                     TKPAVLEVITPTQVRLTISEGRYHQVKRMFAAVGNHVVELHRERIGGITLDADLAPGE
                     YRPLTEEEIASVV"
     gene            15618..17378
                     /locus_tag="OO357_000956"
     CDS             15618..17378
                     /locus_tag="OO357_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5885991.1"
                     /translation="MIFTLRPYQQEAVDATLNHFRRHKTPAVIVLPTGAGKSLVIAEL
                     ARLARGRVLVLAHVKELVAQNHAKYQALGLEADIFAAGLKRKESHGKVVFGSVQSVAR
                     NLDAFQGEFSLLIVDECHRIGDDEESQYQQILTHLTKVNPHLRLLGLTATPFRLGKGW
                     IYQFHYHGMVRGDEKALFRDCIYELPLRYMIKHGYLTPPERLDMPVVQYDFSRLQAQS
                     NGLFSEADLNRELKKQQRITPHIISQIMEFAEKRKGVMIFAATVEHAKEIVGLLPAED
                     AALITGDNPGAERDVLIEDFKAQRFRYLVNVAVLTTGFDAPHVDLIAILRPTESVSLY
                     QQIVGRGLRLAPGKTDCLILDYAGNPHDLYAPEVGTPKGKSDNVPVQVFCPACGFANT
                     FWGKTTADGTLIEHFGRRCQGWFEDDDGHREQCDFRFRFKNCPQCNAENDIAARRCRE
                     CDTVLVDPDDMLKAALRLKDALVLRCSGMSLQHGHDEKGEWLKITYYDEDGADVSERF
                     RLQTPAQRTAFEQLFIRPHTRTPGIPLRWITAADILAQQALLRHPDFVVARMKGQYWQ
                     VREKVFDYEGRFRRAHELRG"
     gene            17503..17787
                     /gene="rplY"
                     /locus_tag="OO357_000957"
     CDS             17503..17787
                     /gene="rplY"
                     /locus_tag="OO357_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HCR5885992.1"
                     /translation="MFTINAEVRKEQGKGASRRLRAANKFPAIIYGGKEAPLAIELDH
                     DKVMNMQAKAEFYSEVLTIVVDGKEIKVKAQDVQRHPYKPKLQHIDFVRA"
     gene            complement(17926..18933)
                     /gene="yejK"
                     /locus_tag="OO357_000958"
     CDS             complement(17926..18933)
                     /gene="yejK"
                     /locus_tag="OO357_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein YejK"
                     /protein_id="HCR5885993.1"
                     /translation="MSLDINQIALHQLIKRDEQNLELVLRDSLLEPTETVVEMVAELH
                     RVYSAKNKAYGLFSEESELAQTLRLQRQGEEDFLAFSRAATGRLRDELAKYPFADGGF
                     VLFCHYRYLAVEYLLVAVLSNLSSMRVNENLDINPTHYLDINHADIVARIDLTEWETN
                     PESTRYLTFLKGRVGRKVADFFMDFLGASEGLNAKAQNRGLLQAVDDFTAEAQLDKAE
                     RQNVRQQVYSYCNEQLQAGEEIELESLSKELAGVSEVSFTEFAAEKGYELEESFPADR
                     STLRQLTKFAGSGGGLTINFDAMLLGERIFWDPATDTLTIKGTPPNLRDQLQRRTSGG
                     N"
     gene            19115..19342
                     /locus_tag="OO357_000959"
     CDS             19115..19342
                     /locus_tag="OO357_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YejL family protein"
                     /protein_id="HCR5885994.1"
                     /translation="MPQISRYSNEQVEQLLAELLNVLEKHKAPTDLSLMVLGNMVTNL
                     INTSIAPAQRQAIANSFASALQSSINEDKAH"
     gene            19362..21122
                     /gene="pbgA"
                     /locus_tag="OO357_000960"
     CDS             19362..21122
                     /gene="pbgA"
                     /locus_tag="OO357_000960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin transport protein PbgA"
                     /protein_id="HCR5885995.1"
                     /translation="MVTHRQRYREKVSQMVSWGHWFALFNILLSLVIGSRYLFIADWP
                     TTLAGRIYSYVSIIGHFSFLVFATYLLILFPLTFIVGSQRLMRFLSVILATAGMTLLL
                     IDSEVFTRFHLHLNPIVWQLVINPDENEMARDWQLMFISVPVILLLELVFATWSWQKL
                     RSLTRRRRFARPLAAFLFIAFIASHVVYIWADANFYRPITMQRANLPLSYPMTARRFL
                     EKHGLLDAQEYQRRLIEQGNPDAVSVQYPLSELRYRDMGTGQNVLLITVDGLNYSRFE
                     KQMPALAGFAEQNISFTRHMSSGNTTDNGIFGLFYGISPSYMDGILSTRTPAALITAL
                     NQQGYQLGLFSSDGFTSPLYRQALLSDFSMPSVRTQSDDQTATQWINWLGRYAQEDNR
                     WFSWVSFNGTNIDDSNQQAFARKYSRAAGNVDDQIKRVLNALRDSGKLDNTVVIITAG
                     RGIPLSEEEETFDWSHGHLQVPLVIHWPGTPAQRINALTDHTDLMTTLMQRLLHVSTP
                     ASEYSQGQDLFNPQRRHYWVTAADNDTLAITTPKKTLVLNNNGKYRTYNLRGERVKDE
                     KPQLSLLLQVLTDEKRFIAN"
     gene            21197..21273
                     /locus_tag="OO357_000961"
     tRNA            21197..21273
                     /locus_tag="OO357_000961"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21231..21233,aa:Pro,seq:ggg)
     gene            complement(21376..23967)
                     /gene="yejO"
                     /locus_tag="OO357_000962"
     CDS             complement(21376..23967)
                     /gene="yejO"
                     /locus_tag="OO357_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter YejO"
                     /protein_id="HCR5885996.1"
                     /translation="MHQSGSVSLCRSAISVLVATALYSPIALASTVEYGETVDGVILE
                     KDIQLVYGTANNTKINPGGEQHIKEFGVSSNTEINGGYQYIEMNGTAEYSVLNDGYQI
                     VQMGGAANQTTLNNGVLQVYGPANDTTIKGGRLIVEKDGGAVFAAIEKGGLLEVKEGG
                     FALAVDQKTGGAIKTTTRAMEVFGTNRLGQFDIKNGIANNMLLENGGSLRVEENDFAY
                     NTTVDSGGLLEVMDGGTATGVDKKAGGKLIVSTNALEVSGTNSKGQFSIKDGVSKNYE
                     LDDGSGLIVMEDTQAIDTILDEHATMQSLGKDTGTKVQANAVYDLGRSDQNGSITYSS
                     KAISENMVINNGRANVWAGTMVNVSVRGNDGILEVMKPQINYAPAMLVGKVVVSEGAS
                     FRTHGAVDTSKADVSLENSAWTIIADITTTNQNTRLNLANLAMSGANVIMMAEPVTRS
                     SVTASAENFITLTTNTLSGNGNFYMRTDMANHQSDQLNVTGQATGDFKIFVTDTGASP
                     AAGDSLTLVTTGGGDAAFTLGNAGGVVDIGTYEYTLLDNGNHSWSLAENRAQITPSTT
                     DVLNMAAAQPLVFDAELDTVRERLGSVKGVSYDTVMWSSAINTRNNVTTDAGAGFEQT
                     LTGLTLGIDSRFSREESSTIRGLFFGYSHSDIGFDRGGKGNIDSYTLGAYAGWEHQNG
                     AYVDGVVKVDRFANTIHGKMSNGATAFGDYNSNGAGAHVESGFRWVDGLWSVRPYLAF
                     TGFTTDGQDYTLSNGMRADVGNTRILRAEAGTAVSYHMDLQNGTTLEPWLKAAVRQEY
                     ADSNQVKVNDDGKFNNDVAGTSGVYQAGIRSSFTPTLSGHLSVSYGNGAGVESPWNTQ
                     AGVVWTF"
     gene            24287..24934
                     /gene="narP"
                     /locus_tag="OO357_000963"
     CDS             24287..24934
                     /gene="narP"
                     /locus_tag="OO357_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite response regulator protein NarP"
                     /protein_id="HCR5885997.1"
                     /translation="MPEATPFQVMIVDDHPLMRRGVRQLLELDPGFEVVAEAGEGASA
                     IDLANRLDIDVILLDLNMKGMSGLDTLNALRRDGVTAQIIILTVSDASSDVFALIDAG
                     ADGYLLKDSDPEVLLEAIRTGAKGSKVFSERVNQYLREREMFGAEEDPFSVLTERELD
                     VLHELAQGLSNKQIASVLNISEQTVKVHIRNLLRKLNVRSRVAATILFLQQRGAQ"
     gene            complement(25145..26197)
                     /gene="ccmH"
                     /locus_tag="OO357_000964"
     CDS             complement(25145..26197)
                     /gene="ccmH"
                     /locus_tag="OO357_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis thiol:disulfide
                     oxidoreductase CcmH"
                     /protein_id="HCR5885998.1"
                     /translation="MRFLLGVLMLMISGSALATIDVLQFKDEAQEQQFRQLTEELRCP
                     KCQNNSIADSNSMIATDLRQKVYELMQEGKSKKEIVDYMVARYGNFVTYDPPLTPLTV
                     LLWVLPVVAIGIGGWVIYARSRRRVRVVPEAFPEQSVPEGKRAGYVVYLPGIVVALIV
                     AGVSYYQTGNYQQVKIWQQATAQAPALLDRALDPKADPLNEEEMSRLALGMRTQLQKN
                     PGDIEGWIMLGRVGMALGNASIATDAYATAYRLDPKNSDAALGYAEALTRSSDPNDNR
                     LGGELLRQLVRTDHSNIRVLSMYAFNAFEQQRFGEAVAAWEMMLKLLPANDTRRAVIE
                     RSIAQAMQHLSPQESK"
     gene            complement(26194..26751)
                     /gene="dsbE"
                     /locus_tag="OO357_000965"
     CDS             complement(26194..26751)
                     /gene="dsbE"
                     /locus_tag="OO357_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbE"
                     /protein_id="HCR5885999.1"
                     /translation="MKRKVLLIPLIIFLAIAAALLWQLARNAEGDDPTNLESALIGKP
                     VPKFRLESLDNPGQFYQADVLTQGKPVLLNVWATWCPTCRAEHQYLNQLSAQGIRVVG
                     MNYKDDRQKAISWLKELGNPYALSLFDGDGMLGLDLGVYGAPETFLIDGNGIIRYRHA
                     GDLNPRVWEEEIKPLWEKYSKEAAQ"
     gene            complement(26748..28691)
                     /gene="ccmF"
                     /locus_tag="OO357_000966"
     CDS             complement(26748..28691)
                     /gene="ccmF"
                     /locus_tag="OO357_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis heme lyase CcmF"
                     /protein_id="HCR5886000.1"
                     /translation="MMPEIGNGLLCLALGIALLLSVYPLWGVARGDARMMASSRLFAW
                     LLFMSVAGAFLVLVNAFVVNDFTVTYVASNSNTQLPVWYRVAATWGAHEGSLLLWVLL
                     MSGWTFAVAIFSQRIPLDIVARVLAIMGMVSVGFLLFILFTSNPFSRTLPNFPIEGRD
                     LNPLLQDPGLIFHPPLLYMGYVGFSVAFAFAIASLLSGRLDSTYARFTRPWTLAAWIF
                     LTLGIVLGSAWAYYELGWGGWWFWDPVENASFMPWLVGTALMHSLAVTEQRASFKAWT
                     LLLAISAFSLCLLGTFLVRSGVLVSVHAFASDPARGMFILAFMVLVIGGSLLLFAARG
                     HKVRSRVNNALWSRESLLLANNVLLVAAMLVVLLGTLLPLVHKQLGLGSISIGEPFFN
                     TMFTWLMVPFALLLGVGPLVRWGRDRPRKIRNLLIIAFISTLVLSLLLPWLFESKVVA
                     MTVLGLAMACWIAVLATAEAALRISRGTKTTFSYWGMVAAHLGLAVTIVGIAFSQNYS
                     VERDVRMKSGDSVDIHEYRFTFRDVKEVTGPNWRGGVATIGVTREGKPETVLYAEKRY
                     YNTAGSMMTEAAIDGGITRDLYAALGEELENGAWAVRLYYKPFVRWIWAGGLMMALGG
                     LLCLFDPRYRKRVSPQKTAPEAV"
     gene            complement(28688..29167)
                     /gene="ccmE"
                     /locus_tag="OO357_000967"
     CDS             complement(28688..29167)
                     /gene="ccmE"
                     /locus_tag="OO357_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HCR5886001.1"
                     /translation="MNIRRKNRLWIACAVLAGLALTIGLVLYALRSNIDLFYTPGEIL
                     YGKRETQQMPEVGQRLRVGGMVMPGSVQRDPNSLKVTFTIYDAEGSVDVSYEGILPDL
                     FREGQGVVVQGELEKGNHILAKEVLAKHDENYTPPEVEKAMEANHRRPASVYKDPAS"
     gene            complement(29164..29373)
                     /gene="ccmD"
                     /locus_tag="OO357_000968"
     CDS             complement(29164..29373)
                     /gene="ccmD"
                     /locus_tag="OO357_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HCR5886002.1"
                     /translation="MTPAFASWNEFFAMGGYAFFVWLAVVMTVIPLVVLVVHSVMQHR
                     AILRGVAQQRAREARLRAAQQQEAA"
     gene            complement(29370..30107)
                     /gene="ccmC"
                     /locus_tag="OO357_000969"
     CDS             complement(29370..30107)
                     /gene="ccmC"
                     /locus_tag="OO357_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmC"
                     /protein_id="HCR5886003.1"
                     /translation="MWKTLHQLAIPPRLYQICGWFIPWLAIASVVVLTVGWIWGFGFA
                     PADYQQGNSYRIIYLHVPAAIWSMGIYASMAVAAFIGLVWQMKMANLAVAAMAPIGAV
                     FTFIALVTGSAWGKPMWGTWWVWDARLTSELVLLFLYVGVIALWHAFDDRRLAGRAAG
                     IMVLIGVVNLPIIHYSVEWWNTLHQGSTRMQQSIDPAMRSPLRWSIFGFLLLSATLTL
                     MRMRNLILLMEKRRPWVSELILKRGRK"
     gene            complement(30149..30811)
                     /gene="ccmB"
                     /locus_tag="OO357_000970"
     CDS             complement(30149..30811)
                     /gene="ccmB"
                     /locus_tag="OO357_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HCR5886004.1"
                     /translation="MMFWRIFRLELRVAFRHSAEIANPLWFFLIVITLFPLSIGPEPQ
                     LLARIAPGIIWVAALLSSLLALERLFRDDLQDGSLEQLMLLPLPLPAVVLAKVMAHWM
                     VTGLPLLILSPLVAMLLGMDVYGWQVMALTLLLGTPTLGFLGAPGVALTVGLKRGGVL
                     LSILVLPLTIPLLIFATAAMDAASMHLPVDGYLAILGALLAGTATLSPFATAAALRIS
                     IQ"
     gene            complement(30808..31425)
                     /gene="ccmA"
                     /locus_tag="OO357_000971"
     CDS             complement(30808..31425)
                     /gene="ccmA"
                     /locus_tag="OO357_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis heme-transporting ATPase
                     CcmA"
                     /protein_id="HCR5886005.1"
                     /translation="MLEARELLCERDERTLFSGLSFTLNAGEWVQITGSNGAGKTTLL
                     RLLTGLSRPDAGEVLWQGQPLHQVRDSYHQTLLWIGHQPGIKTRLTALENLHFYHRDG
                     DAAQCLEALAQAGLAGFEDIPVNQLSAGQQRRVALARLWLTRATLWILDEPFTAIDVN
                     GVDRLTQRMAQHTEQGGIVILTTHQPLNVAESKIRRISLTQTRAA"
     gene            complement(31444..32046)
                     /gene="napC"
                     /locus_tag="OO357_000972"
     CDS             complement(31444..32046)
                     /gene="napC"
                     /locus_tag="OO357_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type protein NapC"
                     /protein_id="HCR5886006.1"
                     /translation="MGNSDRKPGLIKRLWKWWRTPSHLALGTLLLIGFVGGIVFWGGF
                     NTGMEKANTEEFCISCHEMRNTVYQEYMDSVHYNNRSGVRATCPDCHVPHEFVPKMIR
                     KLKASKELYGKIFGVIDTPQKFEAHRLTMAQNEWRRMKDNNSQECRNCHNFEYMDTTA
                     QKSVAAKMHDQAVKDGQTCIDCHKGIAHKLPDMREVEPGF"
     gene            complement(32056..32505)
                     /gene="napB"
                     /locus_tag="OO357_000973"
     CDS             complement(32056..32505)
                     /gene="napB"
                     /locus_tag="OO357_000973"
                     /EC_number="1.9.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase cytochrome c-type subunit"
                     /protein_id="HCR5886007.1"
                     /translation="MKSHDLKKALCQWTAMLALVVSGAVWAANGVDFSQSPEVSGTQE
                     GAIRMPKEQDRMPLNYVNQPPMIPHSVEGYQVTTNTNRCLQCHGVESYRTTGAPRISP
                     THFMDSDGKVGAEVAPRRYFCLQCHVPQADTAPIVGNTFTPSKGYGK"
     gene            complement(32502..33365)
                     /gene="napH"
                     /locus_tag="OO357_000974"
     CDS             complement(32502..33365)
                     /gene="napH"
                     /locus_tag="OO357_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol dehydrogenase ferredoxin subunit NapH"
                     /protein_id="HCR5886008.1"
                     /translation="MANRKRDAGREALEKKGWWRSHRWLVLRRLCQFFVLGMFLSGPW
                     FGVWILHGNYSSSLLFDTVPLTDPLMTLQSLASGHLPATVALTGAVIITVLYALAGKR
                     LFCSWVCPLNPITDLANWLRRRFDLNQSATIPRHIRYVLLVVILVGSALTGTLIWEWI
                     NPVSLMGRSLVMGFGSGALLILALFLFDLLVVEHGWCGHICPVGALYGVLGSKGVITV
                     AASDRQKCNRCMDCFHVCPEPHVLRAPVLDEQSPVQVTSRDCMTCGRCVDVCSEDVFT
                     ITTRWSSGAKS"
     gene            complement(33352..34047)
                     /gene="napG"
                     /locus_tag="OO357_000975"
     CDS             complement(33352..34047)
                     /gene="napG"
                     /locus_tag="OO357_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapG"
                     /protein_id="HCR5886009.1"
                     /translation="MSRSAKPQNGRRRFLRDVVRTAGGLAAVGVALGLQQQTARASGV
                     RLRPPGAINENAFASACVRCGQCVQACPYDTLKLATLASGLSAGTPYFVARDIPCEMC
                     EDIPCAKVCPSGALDREIESIDDARMGLAVLVDQENCLNFQGLRCDVCYRECPKIDEA
                     ITLELERNTRTGKHARFLPTVHSDACTGCGKCEKVCVLEQPAIKVLPLSLAKGELGHH
                     YRFGWLEGNNGKS"
     gene            complement(34054..36540)
                     /gene="napA"
                     /locus_tag="OO357_000976"
     CDS             complement(34054..36540)
                     /gene="napA"
                     /locus_tag="OO357_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="periplasmic nitrate reductase subunit alpha"
                     /protein_id="HCR5886010.1"
                     /translation="MKLSRRSFMKANAVAAAAAAAGLSVPGVARAVVGQQEAIKWDKA
                     PCRFCGTGCGVLVGTQQGRVVACQGDPDAPVNRGLNCIKGYFLPKIMYGKDRLTQPLL
                     RMKNGKYDKEGEFTPITWDQAFDVMEEKFKTALKEKGPESIGMFGSGQWTIWEGYAAS
                     KLFKAGFRSNNIDPNARHCMASAVVGFMRTFGMDEPMGCYDDIEQADAFVLWGANMAE
                     MHPILWSRITNSRLSNQNVTVAVLSTYQHRSFELADNGIIFTPQSDLVILNYIANYII
                     QNNAINQDFFSKHVNLRKGATDIGYGLRPTHPLEKAAKNPGSDASEPMSFEDYKAFVA
                     EYTLEKTAEMTGVPKDQLEQLAQLYADPNKKVISYWTMGFNQHTRGVWANNLVYNLHL
                     LTGKISQPGCGPFSLTGQPSACGTAREVGTFAHRLPADMVVTNEKHRDICEKKWNIPS
                     GTIPAKIGLHAVAQDRALKDGKLNVYWTMCTNNMQAGPNINEERMPGWRDPRNFIIVS
                     DPYPTVSALAADLILPTAMWVEKEGAYGNAERRTQFWRQQVQAPGEAKSDLWQLVQFS
                     RRFKTEEVWPEELLAKKPELRGKTLYEVLYATPEVSKFPVSELAEDQLNDESRELGFY
                     LQKGLFEEYAWFGRGHGHDLAPFDDYHKARGLRWPVVNGKETQWRYSEGNDPYVKAGE
                     GYKFYGKPDGKAVIFALPFEPAAEAPDEEYDLWLSTGRVLEHWHTGSMTRRVPELHRA
                     FPEAVLFIHPLDAKARDLRRGDKVKVVSRRGEVISIVETRGRNRPPQGLVYMPFFDAA
                     QLVNKLTLDATDPLSKETDFKKCAVKLEKV"
     gene            complement(36537..36800)
                     /gene="napD"
                     /locus_tag="OO357_000977"
     CDS             complement(36537..36800)
                     /gene="napD"
                     /locus_tag="OO357_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone NapD"
                     /protein_id="HCR5886011.1"
                     /translation="MHTNWQVCSLVVQAKSERISDISTQLNAFPGCEVAVSDAPSGQL
                     IVVVEAEDSETLIQTIESVRNVEGVLAVSLVYHQQEEQGEETP"
     gene            complement(36790..37284)
                     /gene="napF"
                     /locus_tag="OO357_000978"
     CDS             complement(36790..37284)
                     /gene="napF"
                     /locus_tag="OO357_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-type protein NapF"
                     /protein_id="HCR5886012.1"
                     /translation="MKIDASRRGILTGCWRKASNGIRPPWSGDESHFLTHCTRCDACI
                     NACENNILQRGTGGYPSVNFKNNECSFCYACAQACPESLFSPRHTRAWDLQFTIGDAC
                     LAYQSVECRRCQDSCEPMAIIFRPTLSGIYQAQLNSQLCNGCGACAASCPVSAITAEY
                     LHAH"
     gene            37393..37557
                     /locus_tag="OO357_000979"
     CDS             37393..37557
                     /locus_tag="OO357_000979"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886013.1"
                     /translation="MHTPIGVKPVAGSKEWREAWQKRAFAHISNGYKHIYIAINSPEI
                     FLLVCSLIRI"
     gene            37692..38180
                     /gene="eco"
                     /locus_tag="OO357_000980"
     CDS             37692..38180
                     /gene="eco"
                     /locus_tag="OO357_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708105.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease inhibitor ecotin"
                     /protein_id="HCR5886014.1"
                     /translation="MKTILPEVLFAAFATTSAWAAESVQPLEKIAPYPQAETGMKRQV
                     IQLTPQEDESTLKVELLIGQTLEVDCNLHRLGGKLESKTLEGWGYDYYVFDKVSSPVS
                     TMMACPDGKKEKKFVTAYLGDAGMLRYNSKLPIVVYTPDNVDVKYRVWKAEEKIDNAE
                     VR"
     gene            complement(38302..39333)
                     /locus_tag="OO357_000981"
     CDS             complement(38302..39333)
                     /locus_tag="OO357_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
                     /protein_id="HCR5886015.1"
                     /translation="MNYGEITRQLIASLQKKSNFSLQLSSEVRALKRNDDNTWTVTVA
                     DLKNGTAQNIRAKFVFIGAGGAALKLLQESGIPEAKDYAGFPVGGQFLVSENPDVVNR
                     HLAKVYGKASVGAPPMSVPHIDTRVLDGKRVVLFGPFATFSTKFLKNGSLWDLMSSTT
                     TSNVMPMMHVGLDNFDLVKYLVSQVMLSEEDRFEALKEYYPQAKKEDWRLWQAGQRVQ
                     IIKRDAEKGGVLRLGTEVVSDQQGTIAALLGASPGASTAAPIMLNLLEKVFGDRVSSP
                     QWQATLKAIVPSYGRKLNGDVAATERELQYTSEVLGLKYDKPQAADSTPKPQLKPQPV
                     QKEVADIAL"
     gene            complement(39567..39926)
                     /locus_tag="OO357_000982"
                     /pseudo
     CDS             complement(39567..39926)
                     /locus_tag="OO357_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012906537.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate:quinone oxidoreductase"
     gene            complement(40144..41787)
                     /gene="yojI"
                     /locus_tag="OO357_000983"
     CDS             complement(40144..41787)
                     /gene="yojI"
                     /locus_tag="OO357_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="microcin J25 efflux ABC transporter YojI"
                     /protein_id="HCR5886016.1"
                     /translation="MELLVLVWRQYRWPFISVMALSLASAALGIGLIAFINQRLIETA
                     DTSLLVLPEFLGLLLLLMAVTLGSQLALTTLGHHFVYRLRSEFIKRILDTHVERIEQL
                     GSASLLAGLTSDVRNITIAFVRLPELGQGIILTIGSSAYLWMLSGKMLLVTAIWMAIT
                     IWGGFVLVARVYKHMATLRETEDKLYTDFQTVLEGRKELTLNRERAEYVFNNLYIPDA
                     QEYRHHIIRADTFHLSAVNWSNIMMLGAIGLVFWMANSLGWADTNVAATYSLTLLFLR
                     TPLLSAVGALPTLLTAQVAFNKLNKFALAPFKAEFPRPQAFPNWQTLELRNVTFAYQD
                     NAFSVGPINLTIKRGELLFLIGGNGSGKSTLAMLLTGLYQPQSGEILLDGKPVSGEQP
                     EDYRKLFSAVFTDVWLFDQLLGPEGQPANPQLVEKWLAQLKMAHKLELSNGRIVNLKL
                     SKGQKKRVALLLALAEERDIILLDEWAVDQDPHFRREFYQVLLPLMQEMGKTIFAISH
                     DDHYFIHADRLLEMRNGQLSELTGEERDAASRDAVARTA"
     gene            complement(41863..42513)
                     /gene="alkB"
                     /locus_tag="OO357_000984"
     CDS             complement(41863..42513)
                     /gene="alkB"
                     /locus_tag="OO357_000984"
                     /EC_number="1.14.11.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA oxidative demethylase AlkB"
                     /protein_id="HCR5886017.1"
                     /translation="MLDLFADAEPWQEPLAAGAVILRRFAFNAAEQLIRDINDVASQS
                     PFRQMVAPGGYTMSVAMTNCGHLGWTTHRQGYLYSPIDPQTNKPWPAMPQSFHDLCQR
                     AATAAGYPDFQPDACLINRYAPGAKLSLHQDKDEPDLRAPIVSVSLGLPAIFQFGGLK
                     RNDPLKRLLLEHGDVVVWGGESRLFYHGIQPLKAGFHPLTTDCRYNLTFRQAGKKE"
     gene            complement(42513..43577)
                     /gene="ada"
                     /locus_tag="OO357_000985"
     CDS             complement(42513..43577)
                     /gene="ada"
                     /locus_tag="OO357_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HCR5886018.1"
                     /translation="MKNATCLTDDQRWQSVLARDPNADGEFVFAVRTTGIFCRPSCRA
                     RHALRENVSFYANASEALAAGFRPCKRCQPDKANPRQHRLDKITHACRLLEQETPVTL
                     EALADQVAMSPFHLHRLFKATTGMTPKAWQQAWRARRLRESLAKGESVTTSILNAGFP
                     DSSSYYRKADETLGMTAKQFRHGGENLAVRYALADCELGRCLVAESERGICAILLGDD
                     DATLISELQQMFPAADNAPADLTFQQHVREVIASLNQRDTPLTLPLDIRGTAFQQQVW
                     QALRTIPCGETVSYQQLANAIGKPKAVRAVASACAANKLAIVIPCHRVVRGDGTLSGY
                     RWGVSRKAQLLRREAENEER"
     gene            complement(43651..44706)
                     /gene="apbE"
                     /locus_tag="OO357_000986"
     CDS             complement(43651..44706)
                     /gene="apbE"
                     /locus_tag="OO357_000986"
                     /EC_number="2.7.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase ApbE"
                     /protein_id="HCR5886019.1"
                     /translation="MEISFTRVALLVAALFFVGCDQKPQPAKTHATEVTVLEGKTMGT
                     FWRASIPGIDAKRSAELKEKIQTQLDADDQLLSTYKKDSALMRFNDSQSLSPWPVSEA
                     MADIVTTSLRIGARTDGAMDITVGPLVNLWGFGPEQQPVQIPSQEQIDAMKAKTGLQH
                     LTVINQSHQQYLQKDLPDLYVDLSTVGEGYAADHLARLMEQEGISRYLVSVGGALNSR
                     GMNGEGQPWRVAIQKPTDKENAVQAVVDINGHGISTSGSYRNYYELDGKRLSHVIDPQ
                     TGRPIEHNLVSVTVIAPTALEADAWDTGLMVLGPEKAKEVVRREGLAVYMITKEGDSF
                     KTWMSPQFKSFLVSEKN"
     gene            complement(44818..45939)
                     /gene="ompC"
                     /locus_tag="OO357_000987"
     CDS             complement(44818..45939)
                     /gene="ompC"
                     /locus_tag="OO357_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5886020.1"
                     /translation="MKVKVLSLLVPALLVAGAANAAEVYNKDGNKLDLYGKVDGLHYF
                     SDDKSVDGDQTYMRLGFKGETQVTDQLTGYGQWEYQIQGNSAENENNSWTRVAFAGLK
                     FQDVGSFDYGRNYGVVYDVTSWTDVLPEFGGDTYGSDNFMQQRGNGFATYRSTDFFGL
                     VDGLNFAVQYQGKNGSPEGEGMTNNGREALRQNGDGVGGSITYDYEGFGIGAAVSSSK
                     RTDDQNFGLNRYDERYIGNGDRAETYTGGLKYDANNIYLAAQYTQTYNATRVGNLGWA
                     NKAQNFEAVAQYQFDFGLRPSLAYLQSKGKNLGVINGRNYDDEDILKYVDVGATYYFN
                     KNMSTYVDYKINLLDDNQFTRDAGINTDNIVALGLVYQF"
     gene            46677..49349
                     /gene="rcsD"
                     /locus_tag="OO357_000988"
     CDS             46677..49349
                     /gene="rcsD"
                     /locus_tag="OO357_000988"
                     /EC_number="2.7.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase RcsD"
                     /protein_id="HCR5886021.1"
                     /translation="MRQKETTATTRFSLLPGSITRFFLLLIIVLLVTMGVMVQSAVNA
                     WLKDKSYQIVDITHAIQKRVDTWRYVTRQIYDNIAATTSPSSGEGLQETRLKQDVYYL
                     EKPRRKTEALIFGSHDNSTLEMTQRMSTYLDTLWGAENVPWSMYYLNGQDNSLVLIST
                     LPLKDLTSGFKESTVSDIVDSRRAEMLQQANALDERESFSNMRRLAWQNGHYFTLRTT
                     FNQPGHLATVVAFDLPINDLIPPGMPLDSFRLEPDATATGNNDNEKEGTDSVSIHFNS
                     TKIEISSALNSTDMRLVWQVPYGTLLLDTLQNILLPLLLNIGLLALALFGYTTFRHFS
                     SRSTESVPSTAVNNELRILRAINEEIVSLLPLGLLVHDQESNRTVISNKIADHLLPHL
                     NLQNITTMAEQHQGIIQATINNELYEIRMFRSQVAPRTQIFIIRDQDREVLVNKKLKQ
                     AQRLYEKNQQGRMTFMKNIGDALKEPAQSLAESAAKLNAPESKQLANQADVLVRLVEE
                     IQLANMLADDSWKSETVLFSVQDLIDEVVPSVLPAIKRKGLQLLINNHLKAHDMRRGD
                     RDALRRILLLLMQYAVTSTQLGKITLEVDQDESSEDRLTFRILDTGEGVSIHEMDNLH
                     FPFINQTQNDRYGKADPLAFWLSDQLARKLGGHLNIKTRDGLGTRYSVHIKMLAADPE
                     VEEEEERLLDDVCVMVDVTSAEIRNIVTRLLENWGATCITPDERLISQDYDIFLTDNP
                     SNLTASGLLLSDDESGVREIGPGQLCVNFNMSNAMQEAVLQLIEVQLAQEEVTESPLG
                     GDENAQLHASGYYALFVDTVPDDVKRLYTEAATSDFAALAQTAHRLKGVFAMLNLVPG
                     KQLCETLEHLIREKDVPGIEKYISDIDSYVKSLL"
     gene            49366..50016
                     /gene="rcsB"
                     /locus_tag="OO357_000989"
     CDS             49366..50016
                     /gene="rcsB"
                     /locus_tag="OO357_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsB"
                     /protein_id="HCR5886022.1"
                     /translation="MNNMNVIIADDHPIVLFGIRKSLEQIEWVNVVGEFEDSTALINN
                     LPKLDAHVLITDLSMPGDKYGDGITLIKYIKRHFPSLSIIVLTMNNNPAILSAVLDLD
                     IEGIVLKQGAPTDLPKALAALQKGKKFTPESVSRLLEKISAGGYGDKRLSPKESEVLR
                     LFAEGFLVTEIAKKLNRSIKTISSQKKSAMMKLGVENDIALLNYLSSVTLSPADKD"
     gene            complement(50216..53065)
                     /gene="rcsC"
                     /locus_tag="OO357_000990"
     CDS             complement(50216..53065)
                     /gene="rcsC"
                     /locus_tag="OO357_000990"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RcsC"
                     /protein_id="HCR5886023.1"
                     /translation="MKYLASFRTTLKASRYMFRALALVLWLLIAFSSVFYIVNALHQR
                     ESEIRQEFNLSSDQAQRFIQRTSDVMKELKYIAENRLSAENGVLSPRGRETQADVPAF
                     EPLFADSDCSAMSNTWRGSLESLAWFMRYWRDNFSAAYDLNRVFLIGSDNLCMANFGL
                     RDMPVERDTALKALHERINKYRNAPQDDSGSNLYWISEGPRPCVGYFYALTPVYLANR
                     LQALLGVEQTIRMENFFLPGTLPMGVTILDENGHTLISLTGPESKIKGDPRWMQERSW
                     FGYTEGFRELVLKKNLPPSSLSIVYSVPVDKVLERIRMLILNAILLNVLAGAALFTLA
                     RMYERRIFIPAESDALRLEEHEQFNRKIVASAPVGICILRTADGVNILSNELAHTYLN
                     MLTHEDRQRLTQIICGQQVNFVDVLTSNNTNLQISFVHSRYRNENVAICVLVDVSSRV
                     KMEESLQEMAQAAEQASQSKSMFLATVSHELRTPLYGIIGNLDLLQTKELPKGVDRLV
                     TAMNNSSSLLLKIISDILDFSKIESEQLKIEPREFSPREVMNHITANYLPLVVRKQLG
                     LYCFIEPDVPVALNGDPMRLQQVISNLLSNAIKFTDTGCIVLHVRADGDYLSIRVRDT
                     GVGIPAKEVVRLFDPFFQVGTGVQRNFQGTGLGLAICEKLISMMDGDISVDSEPGMGS
                     QFTVRIPLYGAQYPQKKGVEGLSGKRCWLAVRNASLCQFLETSLQRSGIVVTTYEGQE
                     PTPEDVLITDEVVSKKWQGRAVVTFCRRHIGIPLEKAPGEWVHSVAAPHELPALLARI
                     YLIEMESDDPANALPSTDKAVSDNDDMMILVVDDHPINRRLLADQLGSLGYQCKTAND
                     GVDALNVLSKNHIDIVLSDVNMPNMDGYRLTQRIRQLGLTLPVIGVTANALAEEKQRC
                     LESGMDSCLSKPVTLDVIKQTLTVYAERVRKSRDS"
     gene            complement(53340..54116)
                     /locus_tag="OO357_000991"
     CDS             complement(53340..54116)
                     /locus_tag="OO357_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaP family protein"
                     /protein_id="HCR5886024.1"
                     /translation="MRKIFLPLLLVALSPVAHSEGVQEVEIDAPLSGWHPAEGEDASF
                     SQSINYPASSVNMADDQNISAQIRGKIKNYAAAGKVQQGRLVVNGASMPQRIESDGSF
                     ARPYIFTEGSNSVQVISPDGQSRQKMQFYSTPGTGTIRARLRLVLSWDTDNTDLDLHV
                     VTPDGEHAWYGNTVLKNSGALDMDVTTGYGPEIFAMPAPVHGRYQVYINYYGGRSETE
                     LTTAQLTLITDEGSVNEKQETFIVPMRNAGELTLVKSFDW"
     gene            complement(54121..55770)
                     /locus_tag="OO357_000992"
     CDS             complement(54121..55770)
                     /locus_tag="OO357_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfaQ family protein"
                     /protein_id="HCR5886025.1"
                     /translation="MNWRRIVWLLALVTLPTLAEETPLQLVLRGAQHDQLYQLSSSGV
                     TKVSALPDSLTTPLGSLWKLYVYAWLEDTHQPEQPYQCRGNSPEEVYCCQAGESITRD
                     TALVRSCGLYFAPQRLHIGADVWGQYWQQRQAPAWLASLTTLKPETSVTVKSLLDSLA
                     TLPAQNKAQEVLLDVVLDEAKIGVASMLGSRVRVKTWSWFADDKQEIRQGGFAGWLTD
                     GTPLWVTGSGTSKTVLTRYATVLNRVLPVPTQVASGQCVEVELFARYPLKKITAEKST
                     TAVKPSVLNGRYRVTFTNGNHITFVSHGETTLLSEKGKLKLQSHLDREEYVARVLDRE
                     AKSTPPEAAKAMTVAIRTFLQQNANREGDCLTIPDSSATQRVSASPATTGARTMTAWT
                     QELIYAGDPVHYHGSRATEGTLSWRQATAQAGQGERYDQILAFAYPDNSLSRWGAPRS
                     TCQLLPKAKAWLAKKMPQWRRILQGETGYNEPDVFAVCRLVSGFPYTDRQQKRLFIRN
                     FFTLQDRLDLTHEYLHLAFDGYPTGLDENYIETLTRQLLMD"
     gene            complement(55771..56330)
                     /locus_tag="OO357_000993"
                     /pseudo
     CDS             complement(55771..56330)
                     /locus_tag="OO357_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112876.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
     gene            complement(56489..>58035)
                     /locus_tag="OO357_000994"
     CDS             complement(56489..>58035)
                     /locus_tag="OO357_000994"
                     /inference="COORDINATES: protein motif:HMM:NF012433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5886026.1"
                     /translation="LTSSLKGKPVSAQLTVGVVDEMIYALQPEIAPNIGKFFYPLGRN
                     NVRTSSSLSFISYDQALSSEPVAPGATNRSERRVKMLERPRREEVDTAAWMPSLTTDK
                     QGKAYFTFLMPDSLTRWRITARGMNGDGLVGQGRAYLRSEKNLYMKWSMPTVYRVGDK
                     PAAGLFIFSQQDNEPVALVTKFAGAEMRQTLTLHKGANYISLTQNIQQSGLLSAELQQ
                     NGQVQDSISTKLSFVDNSWPVEQQKNVMLGGGENALMLPEQASNIRLQSSETPQEIFR
                     NNLDALVDEPWGGVINTGSRLIPLSLAWRSLADHQSAAANDIRQMIQDNRLRLMQLAG
                     PGARFTWWGEDGNGDAFLTAWAWYADWQASQALGVTQQPEYWQHMLDSYAEQADNMPL
                     LHRALVLAWAQEMNLPCKTLLKGGDEAIARRGTKDEDFSEEDIRDINDSLILDTPESP
                     LADAVANVLTMTLLKKAQLKSTVMPQVQQYAWDKAANSNQPLAHTVVLLNSGGDATQT
                     AAILMV"
BASE COUNT        14198 a        15655 c        14257 g        13925 t
ORIGIN      
        1 tttgcttact gcggattaaa gatgtttaag gcgcggtcag catttccaga tcgataagcc
       61 aggtcatggc ctgctgacgc gagttaccgc acatttcagc gctggcttgt aagcctgcac
      121 agactttcgg tcgcagtggc gaggtgaaaa ttttacagcg ttgctgttca tcaagctgaa
      181 tgcagggcgt attggcgggc ttgccatctg gcataccggg aatagggctg gaaattgaag
      241 gggcagtgca acacgccccg caacccggac ggcattccat acgttgttct cttaaggtta
      301 accaccggat tgatgagcgc gcacagtacc accttttttg caccagcaaa agtgcgaata
      361 ccacttgccc gaaaggcccg tcgcgagtac tttgtcgcga tatttttgac attttcgact
      421 acaggaattt ttcgatgcca agagcgaacg aaattaaaaa aggtatggta ctgaattaca
      481 acggcaaact gctgctggct aaggatattg atattcagtc gcccactgcc cgcggtgccg
      541 ctacgctgta caaaatgcgt ttttctgatg tccgtaccgg gctgaaagta gaagagcgtt
      601 tcaaaggtga tgatatcgtt gacaccgtga cgctgacccg ccgttacgtt gacttctcct
      661 atgtcgatgg caacgaatat gtctttatgg ataaagaaga ctataccccg tataccttca
      721 ccaaagatca gattgaagaa gagttgctgt ttatgccaga gggcggcatg ccggacatgc
      781 aggtgctgac ctgggacggt caactgctgg cgcttgagct gccgcagacc gttgatctgg
      841 aaatcgttga aaccgcacca ggcatcaaag gggcatctgc cagcgcccgt aacaaaccgg
      901 cgacattgag cactggtctg gtgattcagg taccagaata cttaagcccg ggcgaaaaaa
      961 ttcgtatcca tatcgaagaa cgccgttata tggggcgtgc tgactaactt cagccgcatg
     1021 cagaaaaggg atagctcagg ctgtcccttt tttaatttat tataccaatc ttctattttg
     1081 cgcttcctga acaccttacg ccctcaattt tcactcgttg attgatcgcc ctcacacttc
     1141 atcgcattaa caatccagac caatttcaat tgctgtcata taactttaca ctgtcattgt
     1201 taatttatcg ttactaagac gtgactccta tgaagacaat tgcctccgtt acgctcccgc
     1261 atcatgtaca cgctccacgc tacgatcgcc agcagttgca atcacgtatc gttcattttg
     1321 gctttggcgc ctttcaccgc gctcatcagg cgttactgac cgatcgtgtg ctgaatgccc
     1381 agggcggcga ctgggggatc tgtgaaatca gcttgttcag cggtgatcaa ctgatgagcc
     1441 agctccgcgc acagaaccat ttatataccg tgctggagaa aggtgcggac ggcaatcagg
     1501 tgataattgt cggtgccgtt cacgaatgcc ttaatgcaaa actggattcc ttagcggcaa
     1561 ttattgagaa attttgcgag ccacaggtgg caattgtttc cctgacgatt accgaaaaag
     1621 gctattgtat tgacccggcc accggtgcac tcgacaccag taatccgcgg attattcacg
     1681 atctacaaac ccctgaagaa cctcactccg caccgggtat tctcgtcgaa gcactgaaac
     1741 gccgccgtga gcgcagcctt acaccgttta ccgtgctctc ctgcgacaat attcccgaca
     1801 atggccatgt ggtgaaaaac gcggtgctga gaatggcaga aaaacgttcg ccagaactcg
     1861 ccgggtggat aaaagaacac gtcagttttc cgggaaccat ggtcgaccgc attgttccgg
     1921 ctgcaaccga cgaatcactg gcagaaatca gccagcatct gggggtaaat gatccctgcg
     1981 cgattagctg cgaaccgttt atccagtggg tggttgaaga taacttcatc gctgggcgtc
     2041 ctgcctggga agtcgcaggt gtacaaatgg tgaatgatgt cctgccatgg gaagagatga
     2101 aactgcgtat gcttaatggc agccactctt ttctcgctta tctgggttac ctctcaggat
     2161 tcgcctatat cagtgattgc atgcaggatc gcgtatttcg ctatgctgcc agaacattaa
     2221 tgctggatga gcaagcgccg acactgcaaa ttaaagatgt cgatttaaca caatatgcgg
     2281 ataagttaat tgcacgtttt gctaatccgt cgctgaaaca taagacctgg caaatcgcga
     2341 tggatggcag ccagaaatta ccgcaacgta tgctggcagg tattcgcata catctggggc
     2401 gtgaaacgga ctggtcgttg ctggcattag gcgttgctgg ctggatgcgt tacgtcagcg
     2461 gcgttgatga tgccggaaat gccattgatg ttcgcgatcc gcttagcgat aaaattcgcg
     2521 aacttgttgc gggcagcagc agtgaacaac gcgtaaccgc cctgctttcc ctgcgtgaag
     2581 ttttcggtga tgatctgcca gataacccgc attttgtgca ggccatcgaa caagcctggc
     2641 aacaaatcgt acaattcggc gcacatcagg cgctattaaa caccctcaaa atttaacgat
     2701 ttctgcggtt aaagcggatg aagctcacct tcgtccgctc tccccttctc ttttctgcct
     2761 tttttagcca ggattaacgc tcagttaact taccagagta ttgcggagcc gttgtgacca
     2821 ggaccaacct catcaccggt tttctcggca gcgggaaaac cacgtcgatt cttcatctgt
     2881 tagcccataa agatcccaac gaaaaatggg cggtactggt taatgaattt ggggaagtcg
     2941 gaattgatgg tgctttgctc gccgatagcg gcgcattgct gaaagagatc cccggcggct
     3001 gcatgtgctg cgttaatggt ttacccatgc aggtagggtt gaatacctta ctgcgtcagg
     3061 gaaaaccaga ccgcttgttg atagagccga ccgggctggg ccatccgaaa cagatcctcg
     3121 atctgttaac cgcaccagtc tatgaaccgt ggatagatct gcgcgccacc ttgtgcattc
     3181 tcgatccacg cctgctgctg gacgaaaaaa gcgccagcaa tgaaaacttc cgtgaccagc
     3241 tggctgccgc agacatcatt gtcgccaata aatccgaccg tgcgacgccc gaaagtgagc
     3301 aagcgctaca gcgttggtgg cagcaaaatg gtggcgatcg acaattaatt cacagtgagc
     3361 atgggaaagt tgacggtcat cttctggatt tgccgcgtcg caatttagcc gagttgcccg
     3421 ccagcgccgc gcattctcat cagcatgtcg tgaaaaaagg gttagcagcg ttaagcctgc
     3481 cagagcatca acgctggcgt cgcagtctga acagcgggca aggatatcag gcctgcggct
     3541 ggatattcga cgctgatacg gtattcgaca ccattggcat tctggaatgg gcgcgacttg
     3601 caccggtgga acgcgtcaaa ggcgtgctgc gtattcccga agggctggta cgaatcaacc
     3661 gtcagggcga tgacctgcac attgaaacgc aaaacgttgc gccaccggac agccgtattg
     3721 agctgatttc cagcagcgaa gctgactgga atgccttgca gagcgcgctg ttgaagcttc
     3781 gtttagcgac taccgcgtaa ggttgcctgc gtttttcagt aagataatta gagaaaatat
     3841 gattaaaaat ttgccgcaaa tagtgttgtt gaatattgtc ggccccgcgc tgtttctttc
     3901 ctggtatatt cccgttaatc atggattctg gttgccgatt gatgcggata ttttttattt
     3961 ctttaatcag aaactggtcg aaagtaaggc ctttttatgg ctggttgcat tgactaacaa
     4021 tcgcgccttc gacggttgtt cactgctggc gatgggtatg ttgatgctga gtttctggct
     4081 gaaagaaaac gcccctggca gacgacgtat cgtgattatg ggtctggtca tgctattaac
     4141 tgcagtggta ttaaaccagc tgggtcaggc attaattcct gtaaaacggg ccagcccaac
     4201 attgactttt accgatatta accgcgtcag cgaactgctc tctgttccca cgaaagatgc
     4261 ctcacgagat agctttcccg gcgatcacgg catgatgctg cttatttttt cggcattcat
     4321 gtggcgttat ttcggcaaag ttgcaggcct tatcgccctt attattttta tggtttttgc
     4381 atttcccaga gtaatgattg gcgcacactg gtttactgac atcattgtcg gttcgatgac
     4441 cgtgatattg atcggtttgc cctgggtgtt gctgacgcca ttaagtgatc gattaatcac
     4501 cttttttgac aaatcactac caggaaaaaa caaacatttc caaaacaaat aactcacagt
     4561 aattaacatc atcagggtta tttttatagt gaggataatc ctgatgatgc gcaccgtgct
     4621 ttcatctatc gaacgcaaaa atcattctct aagtaaatga atggattgca tgcgtttcac
     4681 tcaattgtac tttaattgac caaccccgct tattaacttt ctgtatcact ttttcttata
     4741 aaaaatcatg taaaaccgct cgccaagacc gcaccaatcg ggtaatctcg aactcgtttt
     4801 gcctcggcgg tagattatcc tcacagcata taattttgtg cgttagtcca cagatttggc
     4861 cttaaggaat tgtttcaaca tgcccaggta attagtctcg tgtcgcttgg cattttttta
     4921 taacgatatt tgtcgttaag gacttcaagg gaaaacaaac aacatggtca aatctcaacc
     4981 gattttgaga tatatcttgc gcgggattcc cgcgattgca gtagcggttc tgctttctgc
     5041 atgtagtgca aataacaccg caaagaatat gcatcctgag acacgtgcag tgggtagtga
     5101 aacatcatca ctgcaagctt ctcaggatga atttgaaaac ctggttcgta atgtcgacgt
     5161 aaaatcgcga attatggatc agtatgctga ctggaaaggc gtacgttatc gtctgggcgg
     5221 cagcactaaa aaaggtatcg attgttctgg tttcgtacag cgtacattcc gtgagcaatt
     5281 tggcttagaa cttccgcgtt cgacttacga acagcaggaa atgggtaaat ctgtttcccg
     5341 cagtaatttg cgtacgggtg atttagttct gttccgtgcc ggttcaacgg gacgccatgt
     5401 cggtatttat atcggcaaca atcagtttgt ccatgcttcc accagcagtg gtgttattat
     5461 ttccagcatg aatgaaccgt actggaagaa gcgttacaac gaagcacgcc gggttctcag
     5521 ccgcagctaa taaaccgttt ggatgcaatc ccttggctat cctgacgagt taactgaaag
     5581 cactgcttag gcagtgcttt tttgttttca ttcatcagag aaaatgatgt ttccgcgtct
     5641 tgatccaggc tatagtccgg tcattgttat cttttaaatg ttgtcgtaat ttcaggaaat
     5701 taacggaatc atgttcatac gcgctcccaa ttctggacgt aagctcctgc ttacctgcat
     5761 tgtagcaggc gtgatgattg cgatactggt tagctgcctt cagtttttag tggcctggca
     5821 taagcacgaa gtcaaatacg acacactgat tatcgacgta caaaagtatc tcgataccta
     5881 ttttgccgac ctgaaatcca ctactgaccg gcttcagccg ctgaccttag atacctgcca
     5941 gcaggctaac cccgaactga ccgctcgcgc ggcgtttagc atgaatgtcc gtacgtttgt
     6001 gctggtgaaa gataaaaaaa cattctgttc atctgcgact ggtgagatgg acattccact
     6061 caatgaattg attccggcgc tcgacattaa taaaaacgtc gatatggcga tcttacccgg
     6121 cacgccgatg gtgccgaaca aacccgcaat cgtcatctgg tatcgcaacc ctttgctgaa
     6181 aaatagcggt gtgtttgccg ctctgaatct caacctgacg ccttctctct tttatagttc
     6241 acggcaggaa gattacgatg gcgtcgccct cattattggc aatactgcgc tatctacctt
     6301 ttcttcacgt ttgatgaacg ttaatgaatt aaccgacatg ccggtccatg aaactaaaat
     6361 tgcgggcatt cctctgaccg ttcggcttta tgcggatgac tggacatgga acgatgtgtg
     6421 gtacgcattt ttactgggcg gcatgagtgg aactgtcgtt ggcctgcttt gctattacct
     6481 gatgagcgta cgtatgcgcc ccgacagaga aatcatgacc gccatcaagc gcgaacaatt
     6541 ttacgtggcg tatcaaccgg tggtggatac acaagctttg cgggtaacgg gcctggaagt
     6601 actgctacgc tggcggcatc ctgtagcggg agaaattccc ccggatgcct tcattaactt
     6661 tgccgaatcg caaaagatga ttgtgccgct gactcagcac ctgtttgagt taattgcccg
     6721 cgatgccgca gaattagaaa aagtactgcc ggtaggcgtc aaatttggca ttaacattgc
     6781 gccggaccat ctgcacagcg aaagctttaa agcagatatc cagaaactgc tcacttccct
     6841 gcccgcacac catttccaga ttgtgctgga aattaccgag cgcgatatgc tgaaagagca
     6901 agaagccaca caactcttcg cctggctgca ctcggtcggc gtagaaattg ctattgatga
     6961 cttcggcacc gggcacagcg cgcttatcta tcttgagcgt tttacgctcg attatctgaa
     7021 aattgaccgt ggatttatca acgccatcgg tacggaaacg atcacttcac ccgtacttga
     7081 cgcggtgctg acgctggcga aacgtctcaa tatgctgaca gttactgaag gggtcgaaac
     7141 gccggaacaa gcgcgatggc taagcgaacg cggcgttaat ttcatgcaag gctactggat
     7201 tagtcacccg ttaccgctgg acgattttgt tcgctggctg aagaaaccgt atacgccgca
     7261 gtggtaaggt gtgcttacgt cccttattat tcatagtgaa agcatgccgg attgcggcta
     7321 atgatgagta aaaggaaatc cgttgcagat gattgtgcgc atactgctgc tgtttatcgc
     7381 tctgttcacc ttcggtgcgc aggcgcaggc tatcaaggaa agctatacct ttgccgtgct
     7441 gggcgaaccc cggtacgcgt ttaatttcaa ccattttgat tatgtgaacc ccgccgcgcc
     7501 aaaaggtggg cagataacgt tgtcagccct cggtaccttc gataatttca accgctatgc
     7561 actgcgcggc aacccgggcg cacgcaccga gcagctgtac gacacgttat ttacgacttc
     7621 cgatgacgaa ccaggcagtt attacccgct gattgctgaa agtgcacgct atgctgacga
     7681 ttattcctgg gtggaggtcg ctattaatcc gcgcgcccgt tttcatgatg gttctcccat
     7741 tactgcccgc gatgtagagt ttacttttca aaaatttctg accgaaggcg tgccgcaatt
     7801 tcgtctggtc tacaaaggca ccaccgtcaa agccattgcg ccgttaaccg tgcgcattga
     7861 gttagctaaa cccggcaaag aagatatgtt gagtctgttt tcgctgccgg tatttccaga
     7921 aaagtactgg aaggatcaca aacttagcga cccgctcgcc acgcctccgc ttgccagtgg
     7981 tccgtaccac gttacgtcct ggaaaatggg gcaaaatatt gtctattccc gcgtgaaaga
     8041 ttactgggca gcaaacttac cggtaaaccg tggacgctgg aatttcgaca ccattcgcta
     8101 cgattattac ctcgatgata atgtcgcctt tgaagcgttt aaagcaggtg cctttgattt
     8161 gcgtatggaa aacgacgcta aaaactgggc cacgcgttat accggtaaaa atttcgataa
     8221 aaaatacatc atcaaagatg agcaaaagaa cgaatcagcc caggatacgc gctggctggc
     8281 gtttaatatc caacgtgcgg tattcagcga tcgccgggtg cgggaagcaa tcactctcgc
     8341 ctttgacttt gaatggatga acaaggcgtt gttttacaac gcctggagtc gcacgaacag
     8401 ttattttcaa aataccgaat acgcggccag aaattacccc gacgccgcgg agctggtgct
     8461 tctggcacca atgaaaaaag atctaccgcc agaagtcttc acacaaatct accagccgcc
     8521 ggtatctaaa ggcgatggct acgatcgtga caacctgtta aaagccgaca aacttctcaa
     8581 cgaagcgggc tgggtgctga agggtcagca acgcgttaat gccacaacgg gtcagccgct
     8641 cagctttgaa ttattgcttc ccgcaagcag caatagtcag tgggtattgc cgttccagca
     8701 cagcctgcaa cggctgggta tcaatatgga cattcgcaag gtggataact cgcaaatcac
     8761 taaccgcatg cgcaatcgcg actatgacat gatgccgcga ctatggcggg cgatgccgtg
     8821 gcccagttcc gatttacaga tttcctggtc atcggaatat atcaattcca cttataatgc
     8881 ccccggcgtg caaagcccgg ttatcgactc actgatcaac caaattattg ccgcgcaggg
     8941 aaataaagaa aaattactgc cgttggggcg agcactggat cgcgtattaa cgtggaatta
     9001 ttacatgctg ccaatgtggt acatggcgga agaccgtctc gcctggtggg ataaattctc
     9061 ccaacccgct gtacgccctg tttacagcct gggtatcgat acctggtggt atgacgttaa
     9121 taaagcggcc aaactgccgt cagccagaca acagggagag tagatgggcg cttacctgat
     9181 tcgccgtctg ttgctggtga tcccaacgtt atgggctatt atcactatca actttttcat
     9241 cgtgcaaatt gcgcctggcg gtccggtcga tcaggccatc gccgccattg agtttggtaa
     9301 tgccggagta ttacccggcg caggcggtga aggtgttcgt gccagccatg cgcaaacggg
     9361 tgtcggcaat atcagcgaca gtaattaccg tggcggacgc ggattagatc cagaagtgat
     9421 cgctgagatc actcatcgct acggtttcga taagccgatc cacgaacgtt acttcaaaat
     9481 gctctgggac tacatccgct ttgattttgg tgatagcctg tttcgcagcg cctcggtgct
     9541 gacgctgatt aaagacagtc tgccggtttc catcaccctc ggattgtgga gcacgctgat
     9601 tatctatctg gtgtcgattc cgttaggcat tcgcaaagct gtttataatg ggagtcgctt
     9661 tgacgtctgg agtagcgcat ttatcatcat cggctacgcc attccggcct ttttgtttgc
     9721 catcctgctg attgtcttct tcgcgggcgg cagctatttc gacctgttcc ctctacgcgg
     9781 cctggtttcc gctaactttg attcgctgcc gtggtatcag aaaatcaccg attatctgtg
     9841 gcatatcacg ctaccggtgc tggcgacagt gattggtggc tttgcggcgc tgaccatgct
     9901 gaccaaaaac tcattccttg atgaagtgcg caagcaatac gtggtgaccg cgcgtgcgaa
     9961 aggggtaagt gagaaaaaca ttctctggaa acatatgttc cgcaacgcca tgctgctggt
    10021 gattgccggt tttccggcga cgtttatcag catgtttttt accggctcgc tgctgattga
    10081 ggtgatgttt tcactcaatg gtcttggctt actgggctac gaagcgaccg tctcgcgcga
    10141 ttatcctgta atgtttggta ccttgtatat tttcaccctg attggcctgc tgctgaatat
    10201 tgtcagtgat atcagctata cgctggttga tccgcgtata gattttgagg gacgttaatg
    10261 tcgcgactca gccccgtcaa tcaggcccgt tgggcgcgtt ttcgtcataa ccgtcgcggc
    10321 tactggtcgt tatggatttt cctcgttttg tttggtttga gtttgtgttc tgaacttatc
    10381 gctaacgata aaccgttgct ggtgcgttat gacggcagtt ggtatttccc gttgttgaaa
    10441 aactacagcg aaagcgattt tggcggcccg ctggcaagtc aggctgatta tcaggacccg
    10501 tggctgaaac aacggctgga aaataacggc tgggtactgt gggcaccgat tcgctttggt
    10561 gctaccagta tcaactttgc taccgataag cccttccctt ctccaccctc ccggcaaaac
    10621 tggctgggaa cggatgcaaa cggcggcgat gtgctggcac gcattctcta tggcacgcgg
    10681 atctcggttc tgtttggcct gatgctgact ctctgttcca gcgtgatggg cgtgctggcg
    10741 ggggcgctac aaggctatta cggcggtaaa gtcgatctct gggggcaacg ctttattgaa
    10801 gtatggtcgg ggatgccgac gctgtttttg attattttac tttccagcgt cgtacagcct
    10861 aacttctggt ggctgctggc aattactgtc ttgtttggct ggatgagtct ggtcggcgtg
    10921 gtgcgggcgg agtttttacg tactcgtaat ttcgactaca ttcgtgcggc acaggcgctt
    10981 ggcgtcagcg atcgcagtat catcctgcgt catatgttgc ctaatgccat ggtcgcgacc
    11041 ctcacctttt taccgtttat tttatgtagt tcgataacca ccctgacctc gctcgatttc
    11101 ctcggcttcg gtctaccgct cggttcaccg tcactcggtg aactgctgtt acaagggaaa
    11161 aataaccttc aggccccgtg gcttgggatc accgccttct tgtcggtggc gatattattg
    11221 tctttgctga tctttattgg tgaagccgtc cgcgacgcat ttgatcctaa taaggcggtg
    11281 tagcatgacg caaactctgt tagcgattga aaatttgtcg gtgggttttc gccatcagca
    11341 aaccgtacgt acagtagtca atgatgtttc actacagatt gaggctggcg aaacgctggc
    11401 gctggtgggt gagtcaggtt caggcaaaag cgttaccgcg ctgtcaattt tacgcctgct
    11461 cccttccccg ccggttgaat atctctccgg cgatattcgt tttcatggcg aatcgctgct
    11521 tcatgccagc gatcaaacgt tgcgcggtgt acgcggtaat aagatcgcca tgatttttca
    11581 ggaaccgatg gtgtcgttaa atccattgca taccctggaa aaacagcttt atgaagtgct
    11641 ttcactccac cgcgggatgc gtcgggaagc ggctcgtggc gaaattctta actgccttga
    11701 tcgcgtcggt atccgccagg cggcaaaacg gctgacagat tatccgcatc agctctccgg
    11761 cggcgaacgc cagcgggtga tgattgcgat ggcgctgtta acgcgaccgg aattattaat
    11821 tgccgatgaa ccgaccaccg cactggacgt ctctgtccag gcgcagattt tacagctgtt
    11881 gcgcgaactg caaggcgagc tgaatatggg catgctgttt attactcata acctcagcat
    11941 tgtcagaaaa ctggcccacc gcgtggcggt aatgcaaaac ggtcgctgtg tcgagcaaaa
    12001 taacgccgct acgctatttg cctcacccac tcatccttac acacaaaagc tactcaacag
    12061 tgaaccgtca ggcgatccgg taccgttgcc agaacctgcc tcaacgttgc tggatgttga
    12121 acagcttcag gttgccttcc ccattcgcaa agggattttg aagcgcattg tggatcataa
    12181 tgtggtggtg aaaaacatca gttttacgct acgggcgggt gaaacactgg gtttagtggg
    12241 cgagtctggt tccgggaaaa gcacgacggg actggcgctg ctgcgactga ttacctctca
    12301 gggcagcatc gtctttgacg gtcagccact gcaagattta aatcgccgcc agctgttacc
    12361 tactcgtcat cgtattcagg tggtatttca ggatccaaac tcctcgctca acccacgact
    12421 caacgttttg cagattattg aggaaggctt acgggttcac cagccgacgc tttctgccgc
    12481 acaacgcgaa caacaagtga tagccgtgat gcatgaagtg ggattagatc ctgaaacacg
    12541 ccaccgttat ccggcggagt tctctggtgg tcagcgacaa cgtattgcga ttgccagggc
    12601 attaattctt aagccctcgc tgatcatact tgatgaaccg acatcatcac tcgacaaaac
    12661 ggtacaggcg caaatattga cgcttttgaa atcattgcaa caaaagcatc aactggccta
    12721 tttgtttatc agccacgatt tgcacgttgt ccgcgcgtta tgtcatcagg ttatcgtact
    12781 gcgacaaggg gaagtagtgg aacaaggacc gtgcgcgcgc gtgtttgcca caccgcagca
    12841 ggagtatacg cgtcagttac tggcgttgag ctgacgctta aaaaggattg tagtttgaaa
    12901 agggttcggc aattgccaca ccaaaatttt tcaatcggca tgttgcagca aactcgtcct
    12961 ggcgatttac aaacaggcac ggctcacctt cacactccaa caccgaacat ggaacttcga
    13021 tgtcgcttaa cgcctggctt agtttataca tcaccgacca tgcattatca ccatccgggc
    13081 taagcaattt aagcgccacc aggctgttgt caccgcacgg tccattttga ggaatcggtt
    13141 caaccttcga acctgcgaaa ccagcagacc agcgatagtt ctgcggcagt cgatggacga
    13201 ttgagagttg taatgaagtc acttattttc cccggaagta cattcacttc acaatttgtt
    13261 tacatcaatt ttaacacatc atcaacaatc cgtctttata cagatcgtaa agattgatgc
    13321 ctcgcctgcg tggcctctat ggctctgatt taagtaatat ctgcgggcgt ggtcacgttt
    13381 gcggggctat atgtacccgt ttctgcgatc taactcaacc tttttaacta caatgatgtg
    13441 actttttaca taaattgatt ttacataaaa taaacatata tcggggaaat gatagatttg
    13501 tggttgacat gcatcaacaa aggaagcctt ttaccttcct cgttgtgcaa tagatcaccg
    13561 ttttttcggc cgactggcgt acagacagaa gagaatggag ctggttgcgc agaatgcaat
    13621 tgaccaaatc atcggccatg cagagttaaa ggtcgcaaga gaaagcaatg cgccaacaat
    13681 tgcccctatg ccaaaacgga aggttcctgc cagcgaagat gccgttcccg ccatatgggg
    13741 aaactcatca agaatgaccg ccatcgcatt ggatgacacc atcgacacgc agcccacaaa
    13801 cgccgcaacg ccaaccacca gcgaccagaa tcccagcccc agcggcgcac tgatgaccat
    13861 ccacgcagcc ataataaatt gtatccacaa ccccgagcgg aacatattta acgcgccaat
    13921 gcggcggacg aagcggctgt taaagatggt catcacgaac agaaaaacaa tgtttagcgc
    13981 aaagtaataa ccaaagtttt ccggcgcgac gtggttaatt tcaatataaa caaacggtcc
    14041 ggcgcttaag aatgagaaca tcccggcaaa gctgaaacca ctggcaagca tgtagctcag
    14101 gacacgttta tggcggaaca gcgccgcaaa gttaccaata gtggtacgaa tgtgaaatgg
    14161 ctgacgacgc tccggtggta aggtttcttt aatcaggaag aaaatcattg ccgaagccag
    14221 aatcgccgct aatgccagga tccagaagat gtaatgccag ctcagccaca ccagcaccca
    14281 gccgccaact atcggtgcca tcagcagtgc aatggtcgtc accagcatga caaacgacat
    14341 catccgcgag aactcttctt tcgggtaaat atcacgcatc aaagcgttga taaccacgct
    14401 ggccgcagcc gcagccagcc cgtggaagaa acgcatcaca atcagctgat cgatggtttg
    14461 tgccaacgca cacgccaccg cggcggcggc aaacaccagc gtcccgccaa gaaccaccgg
    14521 cttacgcccg aagctgtctg ccatcggccc gtagattaac tgccccaacg caaagcccag
    14581 aatataagta ctgagggtca tctgcgtact gcccgccgat acgccaaact gcgctgaaat
    14641 taccggtagc gcgggcagat acatatcaat cgacagcggc atcaacatgg ccagcaggcc
    14701 aaggataaaa acaatagcaa acgacgaatg ctgtcgggtg gtcacaacgg gctcctgaaa
    14761 gtcattgaaa agttagacga cgctggcaat ttcttcttca gttaacggac gatattcacc
    14821 gggggctaaa tcagcatcca gcgtaatacc gccaatacgt tcacgatgca gctcaaccac
    14881 gtggttaccc acggcggcga acatgcgttt cacctgatga taacgccctt cgctgatggt
    14941 cagacgaacc tgcgttgggg taatcacttc aagcaccgcc ggcttagtga gatctttttc
    15001 gttatgcagc tgcacgcctt tagcaaattg ctctgccgta tcgtcagcta caggtgattc
    15061 cagtgtcacc agataggtct tctcgcaatg atggcgcgga gaagtaatgc ggtgcgacca
    15121 ctgaccatca tcagtcatca gcaccagacc ggtggtatcg atatccagac gtcctgcggc
    15181 gtgcagtttc cacgctaccg gttcatccag aaaatagagc accgtcgggt gatccgggtc
    15241 gtccgtggag caaacatagc cctgaggctt attgagcatg aagtaacgtg gaccgtgttg
    15301 ctgcgccagc gggttgccat cgtaagcgac atcatgttca gggagcagtt tgaacgctgc
    15361 attacgaacg atttcgccat cgacggtgac acgattgccg cggatttcac gcccggcaat
    15421 agcacggcta acgccgagtt gctgtgcgat aaatttatca agtcgcatgt gtgtgatttt
    15481 gcctgtaaaa aacggaggtc gggtaatgac ccgaaaatct gaactgttgt ctgcccagta
    15541 tagcggtcta ataacttccc tcaagggaaa aagaaccgtg gcatactatt agcagaataa
    15601 tctacctacg cgaaaccatg atttttacac tccgcccata tcagcaagaa gccgtggatg
    15661 ccacgctcaa ccattttcgt cgtcataaaa cccctgccgt tatcgtgctg cccaccggcg
    15721 caggtaaaag cctggtgata gcggaactgg cacggctggc gcgtggtcgc gtactggtgc
    15781 tggcacacgt taaagaactg gtggcgcaaa accatgcaaa gtatcaggcg ctggggctgg
    15841 aagccgatat ttttgccgcc gggctaaagc gcaaagagag ccacggtaaa gtggtatttg
    15901 gcagcgttca gtcggtcgcc cgtaatcttg atgcctttca gggtgaattt tcgctgttga
    15961 ttgtcgatga atgccaccgt attggtgacg atgaagagag ccagtatcag caaatcctca
    16021 ctcacctgac taaagtgaat ccccacttac gcctgctggg gctgactgcc acgccttttc
    16081 gattgggcaa aggctggatc taccagttcc attatcacgg catggtacgc ggcgatgaga
    16141 aagccctttt ccgtgactgc atttatgagc tgccgctgcg ttatatgatt aaacacggct
    16201 atctgacgcc gccagaacga ctggatatgc cagtagtgca atacgatttc agccgcttgc
    16261 aggcacagag taacgggctg ttcagcgaag ctgatctcaa ccgtgagctg aaaaaacagc
    16321 aacgcattac gccacatatc atcagccaga tcatggagtt cgccgaaaag cgcaaagggg
    16381 taatgatttt cgccgccacc gttgaacacg caaaagagat tgtgggatta ctgcctgccg
    16441 aagatgctgc actgattact ggcgacaacc ccggcgctga gcgcgatgtg ttaattgaag
    16501 attttaaagc ccagcgtttt cgctatctgg tcaacgtcgc agtactgacc accggatttg
    16561 acgccccgca cgtcgatctt atcgccattc tgcgccctac cgaatcagtg agtctttacc
    16621 aacaaattgt cgggcgcggt ctgcgtctcg ctccgggcaa gactgattgt ttaattcttg
    16681 attatgcggg taatcctcac gatctctacg cgccggaagt tggtacaccg aaaggcaaaa
    16741 gtgacaacgt tccggtacag gttttctgcc ctgcctgcgg ttttgccaac accttttggg
    16801 ggaaaacgac cgccgacggg acattgattg aacactttgg tcgccgctgt cagggatggt
    16861 ttgaagatga cgacggtcat cgcgaacagt gtgacttccg tttccgtttt aaaaattgcc
    16921 cgcaatgtaa cgcagaaaat gatattgccg ctcgccgctg ccgcgaatgt gacacggtac
    16981 tggttgatcc ggatgatatg ttaaaagcgg cgctacgact gaaagatgcg ctggtattac
    17041 gctgtagcgg catgtctttg cagcatgggc acgacgagaa aggcgaatgg ttgaaaatca
    17101 cctattacga tgaagacggc gcggatgtga gtgagcgttt ccgtctgcaa acgcccgccc
    17161 agcgtaccgc cttcgagcag ctttttatcc gcccgcatac gcgcacaccg ggcatccctc
    17221 tgcgctggat caccgccgcc gatatcctcg cccagcaagc cttactgcga catccggatt
    17281 ttgtcgtcgc ccgcatgaaa ggtcagtact ggcaagtgcg tgaaaaagtg ttcgattacg
    17341 aaggtcgttt tcgtcgggcg cacgaattac gcggttaatg gtagttttga ttgatgtata
    17401 aggcgattga gtatagaatc tcgcccgctt ttgcatacgc aaagcagatc acttacctgt
    17461 tgctgggtcg cctgtagcag gattaattta agagagaaag aaatgtttac tatcaacgca
    17521 gaagtacgta aagagcaggg taagggtgcg agccgccgcc tgcgtgccgc taacaagttc
    17581 ccggcaatca tctacggtgg caaagaagca ccgctggcta tcgagctgga tcacgacaaa
    17641 gtcatgaaca tgcaagctaa agctgaattc tacagcgaag ttctaaccat cgttgttgac
    17701 ggtaaagaaa tcaaagttaa agctcaggac gtacagcgtc acccgtacaa accgaagctg
    17761 cagcacatcg acttcgttcg cgcttaattg ctgaataagt tgtaaaaaac cccgctccgg
    17821 cggggttttt tgtatctgca gattatgcct gattacggta ttgctatttt ttgcaggcca
    17881 gataaggcgt tcacgccgca tctggcattc agaacacaaa accgattaat tgccgccaga
    17941 cgtccggcgc tgcaattggt cgcgcaaatt cggcggcgtg cctttaatgg tcaaggtatc
    18001 ggttgctgga tcccagaaga tgcgttcacc taacagcatc gcatcaaagt taatagtcaa
    18061 gccaccgccg ctaccggcaa acttcgtcaa ctgacgcagt gtgctacggt ctgccgggaa
    18121 gctctcttcc agttcgtagc ctttttcagc cgcaaactcc gtgaagctca cttcgcttac
    18181 gcctgccagc tctttcgaca gcgattccag ttcgatctct tcgcctgcct gcagctgctc
    18241 attacagtag ctataaacct gctggcgcac gttctgtcgc tctgctttat ccagttgtgc
    18301 ttcggcagtg aaatcatcaa ccgcttgcaa cagcccacgg ttttgcgctt tcgcgttcaa
    18361 tccttcgctg gccccgagga aatccataaa gaaatccgcc actttgcgcc ctactcgccc
    18421 tttcaggaaa gtgagatagc gggtggactc tggattggtt tcccattcgg ttaaatcgat
    18481 acgcgcaaca atatccgcat gattgatatc aagataatgg gttgggttga tgtcgagatt
    18541 ttcgttaacg cgcatactgc tcaggttgct cagaactgcc accagcaaat actccaccgc
    18601 cagataacgg tagtggcaaa acagcacgaa tccaccgtca gcaaaaggat atttcgctaa
    18661 ttcgtcacgc aggcgaccgg ttgccgcgcg gctaaatgcc agaaagtcct cttcgccctg
    18721 acgctgtaag cgcagcgtct gcgccagttc actctcttcg ctaaacaagc cgtaagcttt
    18781 atttttggcg ctatagaccc gatgcagttc tgccaccatc tcaacgacgg tttctgtcgg
    18841 ttccagcaat gaatcgcgca acaccagctc aaggttctgc tcatcacgct tgataagctg
    18901 gtgcagagca atctggttga tatccagact catgataaac tctcctttaa gaccgggcgg
    18961 tattcaacca ccgccagtta agcgacgcaa taaaagatgg cggtcatttt acagaaggca
    19021 gcgtaacgag aatgatttaa cgcataaaaa agcagaaaaa aaaccgttgc tacggtaata
    19081 tgttgccctt tcatcaacta actgatttcg atttatgcca caaatttccc gctacagtaa
    19141 tgaacaggtt gaacaattgc tcgcagagct gctcaacgta ctggaaaaac ataaggctcc
    19201 gaccgacctt tccctgatgg tgttaggcaa catggtcact aaccttatca acaccagcat
    19261 tgccccggcc caacgccagg caattgccaa ctcttttgcc agcgccttac agtcctctat
    19321 caacgaagac aaagcgcact aagggaaaca gataacaggt tatggtaact catcgtcagc
    19381 gctaccgtga aaaagtctcc cagatggtca gttgggggca ctggtttgca ctgttcaata
    19441 ttctgctttc gctcgtcatt ggcagccgtt acctgtttat cgccgactgg ccgacaacgc
    19501 ttgctggtcg catttattcc tacgtaagca ttatcggcca tttcagcttc ctagtgttcg
    19561 ccacctactt gctgatcctc ttcccgctga cctttatcgt cggctcccag aggctgatga
    19621 ggtttttgtc cgtcattctg gcgacggcgg gaatgacgct attactgatc gatagcgaag
    19681 tctttactcg tttccatctc catcttaatc ccatcgtctg gcagttggtt atcaacccag
    19741 acgaaaatga gatggcgcgc gactggcagc tgatgttcat cagcgtgccg gttattttat
    19801 tgcttgaact ggtgtttgcg acgtggagct ggcaaaagct gcgcagcctg acgcgtcgtc
    19861 gacgcttcgc gcgcccgctg gccgcattct tatttatcgc ctttatcgcc tcgcatgtgg
    19921 tgtatatctg ggccgatgcc aacttctatc gcccgatcac catgcagcgc gctaacctgc
    19981 cgctttcgta cccgatgacg gcgcgacgtt tccttgagaa gcatggcctg cttgatgcgc
    20041 aggagtatca acgccgtctt attgagcaag gtaatccaga cgccgtttcc gtacagtatc
    20101 cgttaagcga actgcgctat cgcgatatgg gcaccgggca gaatgtgctg ttgattactg
    20161 tcgatggcct gaactactca cgcttcgaga agcagatgcc tgcgctggca ggttttgctg
    20221 agcaaaatat ttcgttcacg cgccatatga gctccggcaa cactacagac aacggcatct
    20281 ttggcctgtt ctatggcatc tcgccgagct atatggacgg cattctgtcg acccgtacgc
    20341 ctgcggcatt aattactgcg cttaatcagc aaggctatca gctggggtta ttctcatcag
    20401 atggctttac cagcccgctg tatcgccagg cattgttgtc agatttctcg atgccgagcg
    20461 tacgcaccca atctgacgat cagaccgcca cgcagtggat caactggctg ggccgctacg
    20521 cacaagaaga taaccgctgg ttctcgtggg tttctttcaa tggcactaac attgacgaca
    20581 gcaatcagca ggcatttgca cggaaatata gccgggcggc aggcaatgtc gatgaccaga
    20641 tcaaacgcgt gctcaatgca ctgcgtgatt ctggcaaact ggacaatacg gtggtgatta
    20701 tcactgccgg tcggggtatt ccactgagcg aagaggaaga aacctttgac tggtcccacg
    20761 gtcatctgca ggtgccatta gtgattcact ggccaggcac gccggcgcag cgtattaatg
    20821 ctctgactga tcataccgat ctgatgacga cgctgatgca acgcctgctg catgtcagca
    20881 cacctgccag cgaatattcg caaggtcagg atttgttcaa ccctcaacgc cgtcattact
    20941 gggttactgc tgcggataac gatacgctgg caattaccac cccgaaaaag acgctggtgc
    21001 tgaacaataa cggtaaatac cgcacttaca acttacgtgg tgaaagagtg aaagatgaaa
    21061 aaccacagtt aagtttgtta ttgcaagtgc tgacagacga gaagcgtttt atcgctaact
    21121 gattaattat aaatcagtta gcgaaatatc ttacttgcaa tcggtgtgga aaacggtagt
    21181 attagcagcc acgagtcggc acgtagcgca gcctggtagc gcaccgtcat ggggtgtcgg
    21241 gggtcggagg ttcaaatcct ctcgtgccga ccaaaaatcc caagaaaaaa ccaaccctta
    21301 cggttggttt ttttatatct gcaattaatt cgataaacag accgtgacac atcacagcct
    21361 gtttattttc tgttatcaga acgtccagac cacacccgcc tgagtattcc acggcgattc
    21421 tacccctgcg ccattgccat agctgactga caaatgaccg cttaacgtcg gggtaaacga
    21481 tgaccttatc ccagcctgat aaacgccact ggttccagcc acatcattat taaatttgcc
    21541 atcgtcatta actttcacct ggttagaatc ggcgtattcc tgacgcacgg cggctttcag
    21601 ccagggttcc agcgtcgtac cgttttgcag gtccatgtga tagcttaccg ccgttcccgc
    21661 ttcagcgcgt aatatccggg tatttcccac atccgcgcgc atgccgtttg ataacgtgta
    21721 gtcctgacca tctgtggtaa agccggtaaa ggccagatag ggtctaacac tccacaatcc
    21781 gtcaacccaa cggaacccgc tctcaacatg agcacccgcg ccgttactat tgtaatcgcc
    21841 aaacgctgtt gccccattac tcatcttgcc atggatggtg ttggcaaaac ggtcaacttt
    21901 caccacccca tcaacatagg caccgttctg atgctcccaa ccggcataag cccccagggt
    21961 atagctatcg atattacctt ttccgccgcg atcaaaacca atatcagaat gagagtaacc
    22021 aaagaacaag ccgcgaattg tactgctttc ttcacgggag aaacggctat cgataccgag
    22081 cgtcaggccc gtcaatgttt gctcaaaacc agctcccgca tcagtggtca cgttgttgcg
    22141 ggtgttaatt gccgaactcc acatcaccgt atcgtaacta acgcctttta cgctaccaag
    22201 acgctcacgc acggtgtcca gttctgcatc aaataccagc ggttgtgcgg ctgccatgtt
    22261 cagcacatca gtggttgaag gggtaatttg cgcgcgattc tctgcgaggc tccagctatg
    22321 gttgccatta tccagcaagg tatattcata cgtaccgata tcaacaacgc ctccggcatt
    22381 gcccaacgta aatgcagcat caccgccgcc cgttgttacc agtgtaaggc tatctcctgc
    22441 tgccgggctg gcaccggtgt ccgtcacgaa tattttgaaa tcacctgttg cctgaccggt
    22501 gacgttgagc tgatcgctct gatgattggc catatcggta cgcatataaa aattgccgtt
    22561 tcccgacagg gtattggtgg tcaacgtaat gaaattttcc gcacttgccg tcacagatga
    22621 acgagtcact ggctcagcca tcataatcac atttgcgccg gacatcgcaa ggttggctaa
    22681 gttgaggcga gtgttttggt tcgtcgtagt gatatcggca atgatggtcc atgcgctatt
    22741 ttcgagcgaa acgtccgctt tgctggtatc cacggcacca tgcgttctaa aagaagcgcc
    22801 ctcagaaacc actaccttac ccaccaacat agcgggtgca taatttattt gcggcttcat
    22861 gacctcaaga atgccatcat tccctctgac tgacacgtta accattgtgc cagcccagac
    22921 attagcgcgg ccattgttga ttaccatatt ttcagagatg gcttttgagg aatacgtgat
    22981 acttccattc tggtctgatc gaccgagatc atataccgca tttgcctgca ctttcgtacc
    23041 agtatccttt cccagcgatt gcatagtggc atgctcatca aggatagtgt caatggcctg
    23101 cgtgtcctcc ataacaatga gcccggaacc atcatccagt tcataatttt ttgacacacc
    23161 atcctttata ctaaattggc ctttactgtt tgtaccactc acttccagcg cattcgttga
    23221 gacaattaat ttcccgcctg cttttttatc aacgccagtt gcagtcccgc catccataac
    23281 ctccagtaag ccgccactat ccacagtagt attataagcg aagtcatttt cttcaactcg
    23341 caaacttccg ccgttttcca acaacatatt attagcaata ccattcttga tatcgaactg
    23401 accgagacgg tttgttccga atacctccat ggcccgcgtg gttgttttaa tagcaccgcc
    23461 tgttttctga tctaccgcaa gcgcaaatcc cccctcttta acctccagta gtcctccctt
    23521 ttcgatagcg gcaaaaacgg cccccccatc tttttcaacg attaagcgcc cgcctttaat
    23581 cgtggtatca ttcgctgggc cataaacctg tagcacacca ttattgaggg tagtctggtt
    23641 tgccgcgcca cccatttgaa caatttgata accatcattt aatactgagt attctgcggt
    23701 accattcatt tcaatgtatt gataccctcc gttaatttca gtattactac ttacaccaaa
    23761 ttcttttata tgctgttctc cgccaggatt gattttcgta ttattggcgg tcccataaac
    23821 cagctggata tctttttcca ggataacacc atcaactgtc tcaccatact caactgttga
    23881 tgccaatgct atgggtgaat ataacgctgt agccaccaga acagatattg cggaacgaca
    23941 aagagaaaca gaaccagatt gatgcattga gctttcatcc tatgaaatta attgcagtta
    24001 aaagcattgg gtacagaaaa tacccatagc tccatacccg gagtcagttt ttaaaaactg
    24061 tttaaagaaa tgcacaagta ttgtgattga ttttttagtt gtttttcttg atgagaagct
    24121 gatgcaaaat tccgtcttta taataaaaat gatgccaaag cgaacgacaa ggttgtagtt
    24181 ttcactacat gtccatacat aaaatggggt aacattcacg cgcctggtag cgttaccaac
    24241 gctacgctca aacataatga ttctaataaa acctcaggag actactatgc ctgaagcaac
    24301 gccttttcag gtgatgattg tggatgatca tccacttatg cgacgcggtg ttcgtcagtt
    24361 actggagctt gatcctggct ttgaagtggt cgccgaagcg ggcgaaggcg cgagcgcgat
    24421 cgatctggcg aatagactgg atatcgacgt gatcttgctg gatctcaata tgaaaggtat
    24481 gagtggcctg gataccctca atgccttgcg cagggatggc gttaccgcgc aaattattat
    24541 cctgaccgta tccgatgcct ccagcgatgt ctttgcgctg atagacgcag gcgcagacgg
    24601 ttatctgttg aaagacagcg acccggaagt attgctggaa gcgattcgta ccggagcgaa
    24661 aggcagcaaa gtctttagcg aacgcgtcaa tcagtactta cgtgaacgtg aaatgtttgg
    24721 cgcggaagaa gatcccttca gcgtgctgac ggagcgcgag ctggatgttc tgcacgagct
    24781 ggcacagggg ctgtcaaata aacagattgc atcggtgttg aatatttccg agcagacagt
    24841 aaaagtacat attcgcaatc tgctgcgtaa actcaatgtc cgctcacgcg tggcggccac
    24901 cattctgttc ctgcaacaac gcggggcaca ataaaaatag cccgatggat ttatcatcgg
    24961 gctgagattt atgacaaacg caaaactgcc tgatgcgcta cgcttatcag gcctacgtgg
    25021 atcgatcaat ttattgaatt tacacaattt tgtaggccgg ataaggcgtt cacgccgcat
    25081 ccggcataaa ctaagcgcac tttgtcatca gtctagcccg atggcatcac catcgggcct
    25141 ctttttattt actctcctgc ggcgacaaat gttgcatcgc ctgcgcgata ctacgttcaa
    25201 tcaccgcacg gcgagtatcg ttggcaggta agagtttcaa catcatctcc cacgcggcaa
    25261 cggcttcgcc aaatcgctgc tgctcaaagg cattaaacgc atacatgctt agcacacgga
    25321 tattgctatg gtccgttctc accagctgac gtagcagttc accgccgagg cggttgtcgt
    25381 tgggatcaga tgaacgagtc aacgcttcag cgtatcccag tgcggcatcg ctgttcttcg
    25441 gatcgaggcg atacgcagta gcgtatgcat cggtggcgat actggcgtta cccagcgcca
    25501 tgccaacgcg gcccaacata atccagcctt ctatatctcc cggatttttt tgcagttgag
    25561 tacgcatccc cagcgcaagg cgcgacatct cttcttcgtt gagcggatcg gctttcgggt
    25621 ccagcgccct gtccagcaac gccggagcct gtgccgtggc ctgctgccag attttcacct
    25681 gctgataatt gccagtctgg tagtagctga cgccagccac aattaacgcc accacaatac
    25741 ccggcagata aacaacatat ccggcacgct taccttccgg cacgctttgt tcaggaaacg
    25801 cttccggcac cacgcgtacc cgacgccgcg agcgggcgta tatgacccaa ccgccaatgc
    25861 caatagccac tactggcagc acccacagca gcacggtcag cggcgttaac ggcggatcgt
    25921 aagtgacgaa gttgccgtaa cgcgccacca tataatcgac aatctctttt ttacttttac
    25981 cttcctgcat cagttcatac actttctgac gcaggtcggt ggcaatcatc gagttggaat
    26041 cggcaatgct gttgttctgg catttcgggc agcgcagttc ttcagtgagc tgacggaact
    26101 gttgttcctg tgcttcatct ttaaactgca acacgtcgat ggtcgccagc gctgagccgg
    26161 agatcatcag catcagcacg cccaataaaa acctcattgt gcggcctcct tactgtattt
    26221 ctcccacagc ggcttgatct cttcttccca gacgcgagga ttgagatcgc ccgcatggcg
    26281 atagcgaatg ataccgttgc cgtcaataag gaaggtttct ggcgcgccat agacaccgag
    26341 atccagccct aacatgccgt cgccatcaaa caggcttagc gcgtaaggat tgcccagctc
    26401 tttcagccag ctgattgcct tctggcgatc gtctttatag ttcatgccga ccacgcgaat
    26461 gccctgcgct gaaagctgat tcagatattg atgttccgca cggcaggtcg gacaccaagt
    26521 cgcccagacg ttaagcagta ctggtttgcc ctgagtcagc acatccgcct gataaaactg
    26581 ccccggattg tccagtgatt cgagacgaaa cttcggcaca ggcttgccaa tgagcgccga
    26641 ttccagattg gtcggatcat ccccttcggc attacgcgcc agctgccaca gcagcgccgc
    26701 ggcaatcgcc aggaagataa tcaacggaat taacaatact ttgcgcttca tacggcctcc
    26761 ggcgcagttt tttgcggact cacgcgctta cgatagcgag gatcaaacag acacagcagt
    26821 ccgcccaacg ccatcatcag cccgcccgcc caaatccagc gaacaaatgg tttgtagtaa
    26881 agacgcacgg cccacgcgcc gttttccagc tcttcaccga gcgcggcgta caggtcacgc
    26941 gtgatgccgc cgtcaattgc cgcttcggtc atcatcgacc cggcagtgtt gtaataacgt
    27001 ttttccgcat acagcaccgt ttccggttta ccctctcgcg ttacgccaat agtcgccaca
    27061 ccgccgcgcc agttcgggcc agtcacctct ttgacatcac ggaaggtgaa gcgatattca
    27121 tgaatatcga cgctatcgcc ggacttcatg cgcacatcac gctcaacgct ataattctgg
    27181 ctaaaggcaa tgccaacaat tgtcactgcc agccccaggt gagccgccac catcccccaa
    27241 taactgaagg tggttttcgt gccgcgtgaa atacgcagcg cagcttccgc agttgccagc
    27301 accgcaatcc agcaggccat tgccaggccg agcaccgtca tcgccacaac tttgctttcg
    27361 aacagccacg gcaacagcag agacagcacc agcgtagaga tgaaggcgat aatcaataaa
    27421 ttgcggattt tacgtgggcg atcgcgcccc cagcgcacca gaggaccgac accaagcagt
    27481 agcgcaaacg gcaccatcag ccaggtaaac atggtgttga agaacggttc gccaatcgaa
    27541 atactgccca gtccaagttg cttgtgcacc agcggcagca gcgtccccag caacaccacc
    27601 agcatcgcgg cgaccagcaa aacgttgttc gctaacagca aagattcccg cgaccacagc
    27661 gcattgttta cgcgtgagcg aactttgtgt ccacgcgcgg caaacagcag cagcgaaccg
    27721 ccaatcacca gcaccataaa ggcgaggata aacataccgc gcgccggatc agacgcaaac
    27781 gcgtgtaccg ataccagcac gccggaacgc acgaggaaag tccccaacag acacaacgag
    27841 aaggcactga ttgccagcag taatgtccac gctttgaagc tggcgcgttg ttcagtgacc
    27901 gccagtgagt gcatcagtgc agtccccacc agccacggca taaacgaggc gttttctacc
    27961 ggatcccaga accaccagcc accccagccg agttcgtaat aggcccatgc ggaaccgagc
    28021 acaatgccga gcgttaggaa aatccacgcc gccagcgtcc acgggcgagt aaaacgcgca
    28081 taagtgctgt ccagacgccc gctcagcaaa gaagcaatgg caaaagcaaa cgccaccgag
    28141 aaacccacgt accccatata aagcagaggc ggatggaaga tcagccccgg atcctgtaac
    28201 agcgggttaa gatcgcgccc ttcaatcggg aagttcggca gcgtgcgtga gaacgggtta
    28261 gaggtaaaga gaatgaacag caaaaagccg acgctgacca tccccattat cgccagtaca
    28321 cgggccacaa tatccagcgg aatacgctga ctaaaaatcg ccaccgcaaa ggtccagccg
    28381 ctcatcagca gcacccacag cagaagcgaa ccttcatgcg cgccccaagt agccgccacg
    28441 cgataccaca ccggaagctg ggtattggag ttgctggcaa cataggtgac ggtaaagtcg
    28501 ttgaccacga aagcattgac cagcaccaga aatgcgccag ccacagacat aaacagcagc
    28561 caggcaaaca agcgggaaga cgccatcatg cgcgcatctc cgcgcgccac gccccatagc
    28621 ggatacacgg acagcagcag cgcaatccct aacgccaagc acagcagccc gtttccaatt
    28681 tccggcatca tgatgctggg tccttataaa cactcgccgg gcgacggtgg ttagcttcca
    28741 tcgctttctc aacttctggc ggcgtgtagt tttcgtcgtg tttcgccagc acttctttcg
    28801 cgaggatatg attgcctttt tccagctcgc cctgcaccac aacgccctgc ccttcacgga
    28861 acagatccgg caaaatgcct tcgtaagaga catccactga gccttcagca tcgtaaatgg
    28921 tgaaggtcac tttcagcgaa ttgggatcgc gctgcacgct acccggcatc accatcccgc
    28981 caacgcgcag acgctgaccg acttccggca tctgctgagt ttcacgcttg ccgtagagaa
    29041 tttcccccgg cgtataaaag agatcgatat tcgagcgcag cgcatatagc accagaccga
    29101 tagtcagcgc tagccctgcc aacacggcac aggcaatcca caagcggttt ttacggcgaa
    29161 tattcatgca gcctcctgct gttgcgcagc acgtaaacgc gcctcacgcg cccgctgttg
    29221 cgccacacca cgcagaattg cgcgatgttg catcaccgag tgcacgacca aaaccaccag
    29281 cggaataacg gtcatcacca ccgccagcca gacaaaaaag gcgtaaccgc ccattgcgaa
    29341 aaattcattc caggaagcaa atgcaggggt catttacggc ctcttttcag tatcagttca
    29401 ctcacccacg gacggcgttt ttccatcagc aaaatcaaat tacgcatccg catcagcgtc
    29461 agcgtggcag acaggagcag gaagccaaaa atcgaccagc gcagcggcga acgcatcgcc
    29521 ggatcgatac tttgctgcat ccgcgttgat ccctgatgca gggtgttcca ccactccacg
    29581 gagtaatgaa taatcggcag attcaccacg ccaatcagca ccatgatacc tgccgcacgg
    29641 cccgccagac ggcggtcgtc gaaggcgtgc cacagggcaa tcacacccac atacaaaaac
    29701 agcagcacca gttcagaagt cagacgtgca tcccataccc accaggtgcc ccacatcggt
    29761 tttccccatg cagagccggt aaccagggca ataaaggtaa acacggcacc aatgggggcc
    29821 atcgccgcca ccgccaggtt ggccattttc atctgccaga caaggccaat aaacgccgcc
    29881 accgccattg atgcataaat gcccatcgac cagatcgccg caggcacatg caggtagata
    29941 atgcggtagc tatttccctg ctgataatca gccggagcaa agccgaatcc ccagatccag
    30001 ccgacggtaa gcacgaccac actggcaatt gccagccacg gtataaacca gccacagatt
    30061 tgatacagcc gtggtgggat cgccagttga tgcagtgttt tccacatagt ttcgatacca
    30121 gactcgaaca aaaatcagta atccagcgtt attgaatgct gattcgtaac gctgccgccg
    30181 tcgcaaaagg acttaatgtc gcggtgcctg ccagcaacgc gcctaaaatt gccagatacc
    30241 catcaacggg caaatgcata gaagccgcgt ccatcgcggc ggtggcaaag atgagtaatg
    30301 ggatagtcag cggtaacacc agtatgctga gcagcacacc accgcgctta agtcccactg
    30361 tcagcgccac gcccggtgca ccgagaaagc caagcgtagg cgttcccagc agcagcgtca
    30421 gcgccatcac ttgccagcca taaacatcca ttcccagtag cattgctacc agtggcgaaa
    30481 ggatgagtaa cggcagaccg gttaccatcc agtgcgccat caccttcgcc agcacaacgg
    30541 cgggcaaggg taacggcaac aacatcaatt gttcaagact gccgtcctgc aaatcgtcac
    30601 ggaacagtcg ttccagcgcc agcaaggatg aaagcagcgc agcaacccag ataatgcccg
    30661 gtgcaatacg cgccagcagt tgcggctccg gaccgatact gagcggaaaa agggtaatta
    30721 caatcaggaa gaaccacagc gggttggcga tttcggcgct atggcgaaac gctacgcgca
    30781 gttcaagacg gaaaatacgc cagaacatca tgcggccctc gtttgcgtca gtgaaatgcg
    30841 gcgaatttta ctttcagcaa cgttgagcgg ctggtgggta gtcagaatca caatcccccc
    30901 ctgctccgta tgctgcgcca tacgctgggt cagacgatcg acgccgttaa cgtcaatcgc
    30961 ggtaaaaggc tcgtcgagga tccataatgt ggcacgggtc aaccacagac gcgctaaagc
    31021 gacgcggcgt tgttgcccgg cagaaagttg atttaccgga atatcttcga atccggcaag
    31081 cccggcctgc gccaaggctt ccagacattg tgcggcatcg ccatcgcgat gataaaagtg
    31141 cagattttct aacgccgtca gccgggtttt gatccccggc tgatggccta tccataacag
    31201 ggtttgatgg tagctgtcgc gtacctgatg caagggctgc ccttgccaga gaacctcgcc
    31261 agcgtcaggg cgagacaacc ccgtcagcaa acggagaagc gttgtcttcc ccgcgccgtt
    31321 gctaccggtg atttgtaccc actctcctgc gttcagcgta aatgacaagc cactaaataa
    31381 ggttcgttca tcccgctcac aaagtaactc tctggcttca agcataccca cgcaataacc
    31441 ctgttaaaaa cctggctcga cttcacgcat atcgggcagc ttgtgcgcga tccctttatg
    31501 gcaatcaata caggtttgcc catctttcac cgcctggtca tgcatcttcg cggcaaccga
    31561 tttctgggcg gttgtatcca tatactcgaa gttgtgacag ttacggcact cctgcgagtt
    31621 attgtccttc atgcgccgcc actcattctg tgccatcgtc agacgatgag cttcaaattt
    31681 ctgcggcgtg tcaataacgc caaaaatttt accatacagc tctttacttg ctttgagctt
    31741 gcgtatcatc ttcggcacaa actcgtgcgg aacgtgacaa tccggacagg tcgcacggac
    31801 gccgctacgg ttgttgtagt gcacggaatc catgtattcc tgatacaccg tgttgcgcat
    31861 ttcgtggcag ctaatgcaga actcttcggt attggctttt tccatcccgg tgttaaagcc
    31921 cccccagaag acgatgccgc caacaaaacc gatcaacagc agcgtcccca gcgccagatg
    31981 gctgggggta cgccaccatt tccacaggcg cttaatcaga ccaggcttac ggtcagaatt
    32041 tcccataata acctcttatt tcccgtaacc ttttgatggg gtaaaggtat tccccacgat
    32101 tggcgcggta tcggcctgcg gtacgtgaca ttgcagacag aaataacgac gcggagccac
    32161 ttccgcgccc actttgccgt cgctgtccat aaagtgagta ggactgatac gcggcgcgcc
    32221 agtggtgcga tagctttcga caccgtggca ctgcaagcag cgattggtat tggtcgttac
    32281 ctgataacct tcaacgctat gcgggatcat tggcggctga ttcacatagt tcagcggcat
    32341 ccgatcctgc tcttttggca tccgaatggc cccttcctgc gtcccggata cttccggcga
    32401 ctggctaaaa tcgacgccat tagccgccca aaccgcgccg cttaccacca gggccagcat
    32461 cgccgtccat tgacacagcg ctttcttcag gtcatggctt ttcatgattt cgctcccgaa
    32521 ctccatcgtg tagttattgt aaatacatcc tcagaacaga catccacgca gcgaccgcac
    32581 gtcatgcaat cgcggctggt gacctgcacc gggctttgtt catccagcac cggggcacgt
    32641 agcacatgcg gttccgggca aacatgaaaa caatccatac agcggttaca tttctgccga
    32701 tcgctggccg caacggtaat cacacctttg ctacccagca cgccatacag cgcgccaacc
    32761 ggacaaatgt gcccgcacca gccgtgttca acgaccagta gatcaaataa aaacagcgcg
    32821 agaataagca gcgcgccgct gccgaagccc attaccaggc tacgccccat cagagaaacg
    32881 gggttgatcc attcccaaat gagcgtgcca gttaaggctg atcccaccag aatgaccacc
    32941 aacagcacgt agcgaatgtg gcgagggatc gtcgcagact gattgaggtc aaagcgcctg
    33001 cgtaaccagt tcgctaagtc ggtaatcggg ttcagcgggc agacccagct gcaaaataat
    33061 cgctttcccg ccagggcgta gagcacggtg ataatcaccg caccggtcaa cgccaccgta
    33121 gcaggcagat gaccactggc aagactttgc agcgtcatca gcggatcggt cagcggaacg
    33181 gtgtcgaata acaggctact gctgtagttg ccgtgcaaga tccacacacc aaaccatgga
    33241 ccgctcaaaa acatccccag cacgaagaac tgacaaaggc gacgtaacac cagccaacgg
    33301 tgactgcgcc accagccttt tttctccagc gcctcgcgcc cggcgtcacg tttacgattt
    33361 gccattgttc ccctccagcc agccgaagcg gtaatggtga cctaactccc ctttcgccag
    33421 tgacagcggt aacaccttga ttgccggttg ttccagcacg catacttttt cgcacttacc
    33481 gcaaccggta caggcgtcgc tgtgaaccgt cggcagaaag cgggcgtgct taccggtacg
    33541 cgtgttgcgc tccagctcca aggtgatggc ctcatcaatt ttcgggcatt cgcgataaca
    33601 aacatcgcag cgcagcccct gaaagttgag acagttttcc tggtccacca gtaccgccag
    33661 ccccatccgc gcgtcgtcga tcgattcaat ctcacgatcc agcgcaccgc ttgggcacac
    33721 tttggcgcac ggaatgtcct cacacatttc gcaaggaata tcccgtgcga caaaatatgg
    33781 cgtgcccgcc gacagaccag aggccagcgt cgccagtttt aaggtgtcgt aagggcaagc
    33841 ctgaacacac tgaccgcaac gcacacaggc actggcaaag gcgttctcgt ttatggctcc
    33901 gggcgggcgc aaccgcacgc cagatgcgcg tgcggtttgc tgttgtaacc ccagcgccac
    33961 acccacggca gccagcccgc ctgctgtgcg aacgacatcg cgcagaaagc ggcggcgacc
    34021 attttgaggt ttcgctgacc gggacataat ggcttacacc ttctccagtt tgaccgcgca
    34081 cttcttgaag tccgtctctt tcgagagcgg atcggtcgca tccagcgtca gtttgttaac
    34141 cagctgtgcg gcgtcgaaga acggcatgta caccaggccc tgtggcggac ggttacgacc
    34201 gcgcgtttca acaatcgaga tcacttcgcc acggcgagaa accactttca ctttgtcgcc
    34261 acggcgcaga tcgcgcgctt tcgcatccag cgggtgaata aacaagaccg cttccgggaa
    34321 ggcgcggtgc agttccggca cacggcgagt catactgccg gtgtgccagt gctccagaac
    34381 gcgtccggta gagagccaca ggtcgtactc ttcatccggt gcttccgccg ccggttcgaa
    34441 cggcagtgcg aagatcaccg ctttgccatc cggtttaccg tagaacttat agccttcgcc
    34501 cgctttcacg tacgggtcgt taccttcgct gtaacgccac tgtgtttctt taccattcac
    34561 caccggccag cgcagaccgc gcgctttgtg gtagtcatcg aacggtgcca gatcgtgacc
    34621 gtgaccgcga ccaaaccatg cgtactcttc gaacagccct ttttgcagat agaagcccag
    34681 ctcgcgggat tcatcgttca gctgatcttc cgccagttcg gataccggga atttgctcac
    34741 ttcgggggtg gcatacagaa cttcgtacag cgttttgcca cgcagttccg gtttcttcgc
    34801 cagcagctct tccggccata cttcttcagt tttgaagcgg cgggagaact ggactaactg
    34861 ccagagatcc gatttcgctt cgcctggcgc ctgtacctgc tgacgccaga actgagtacg
    34921 gcgttcggcg ttaccgtaag cgccctcttt ctctacccac attgcggtcg gcaggatcaa
    34981 gtcggcggcc agcgcactga ctgtcggata cggatcggag acgatgatga agttgcgcgg
    35041 atcgcgccag cccggcatac gctcttcatt gatgttcggc ccggcctgca tgttgttggt
    35101 acacatggtc cagtaaacat tcagcttgcc gtctttcagc gcacggtctt gtgccaccgc
    35161 atgaagaccg attttcgccg gaatggtgcc gctcgggata ttccacttct tctcacagat
    35221 atcacgatgt ttctcgttag ttaccaccat gtccgcaggc agacggtgag caaaggtgcc
    35281 cacttcacgc gcagtaccac acgcagaagg ttgcccggtc agggagaacg gaccgcaacc
    35341 cggctgggaa attttgccgg tcagcaggtg caggttgtag accaggttgt tagcccacac
    35401 accacgggta tgctggttga agcccatcgt ccagtaggag atgactttct tgttcggatc
    35461 ggcatacagc tgcgccagtt gttctaactg gtctttcggc acgccggtca tttcagcggt
    35521 tttttccagc gtatactcag caacgaaggc tttgtaatct tcaaagctca tcggttcgga
    35581 ggcgtcagaa cccggattct tcgctgcttt ttccagcgga tgggtcggac gtaaaccgta
    35641 gccgatgtcc gtcgcccctt tgcgcaggtt aacgtgcttg ctgaagaagt cctgatttat
    35701 cgcattgttt tgaatgatat agttggcgat gtagttcagg atcaccaggt cagattgcgg
    35761 cgtaaagatg atgccgttat ccgccagctc gaagctacga tgctggtagg tagaaagcac
    35821 cgccacggtg acgttctggt tagagagacg actgttagtg atgcgtgacc agaggatcgg
    35881 gtgcatctcc gccatgttag cgccccacag cacaaacgcg tcagcctgct cgatgtcgtc
    35941 atagcagccc atcggctcat ccataccaaa ggtacgcata aagccaacta ctgccgacgc
    36001 catacagtga cgtgcgttcg ggtcgatgtt gttcgaacgg aagcccgctt tgaacagctt
    36061 ggacgcggca taaccttccc agatagtcca ctgaccagaa ccgaacatac cgatcgattc
    36121 cggccctttt tctttcaggg cggttttgaa tttctcttcc atcacatcga aggcctgatc
    36181 ccaggtgatt ggggtaaatt cgccttcttt gtcatattta ccgtttttca tacgcagcag
    36241 cggctgcgtc aaacggtctt taccgtacat gatcttgggc aggaaatagc ccttaatgca
    36301 gttcaggcca cggttaaccg gtgcgtccgg gtcgccctga caggccacca cacgcccctg
    36361 ctgcgttccg accagaacgc cgcaaccagt accgcagaaa cggcacggcg ctttatccca
    36421 tttaatggct tcctgctgac caacaacggc gcgggcaacg cccggcacgc tgagaccggc
    36481 agccgccgca gcggccgcaa cggcgttagc tttcataaag ctacgacgac tgagtttcat
    36541 ggtgtttcct caccttgctc ttcctgctgg tgataaacca gcgacaccgc cagcacgccc
    36601 tctacgttgc gtactgactc aatggtttgg atcagcgttt cgctgtcttc tgcttccacc
    36661 accacaatca actgaccgct cggcgcgtcg ctgacagcaa cttcacagcc gggaaaggcg
    36721 ttcagttggg tgctgatgtc tgaaattcgt tcgcttttgg cctgcacgac caggctgcaa
    36781 acttgccagt tagtgtgcat ggagatactc cgcagttatg gctgataccg gacagctggc
    36841 tgcacatgct ccacatccgt tgcaaagttg gctattaagt tgcgcctggt agatcccgga
    36901 gagcgtcggg cgaaagataa ttgccattgg ctcacagcta tcctgacagc ggcggcattc
    36961 aactgactga tacgccaggc acgcgtcccc gatggtgaac tgcaaatccc aggccctggt
    37021 gtggcgcgga gaaaatagcg attcggggca agcctgcgcg caggcgtaac agaagctgca
    37081 ctcgttattt ttgaaattaa cgctcggata gccacccgtg ccgcgttgca gaatgttgtt
    37141 ttcacaagca ttaatacagg cgtcgcagcg ggtgcaatgg gtcagaaaat gagattcatc
    37201 accggaccag ggcggacgga taccgttact ggctttgcgc cagcaaccag tgagtatgcc
    37261 ccgacgggat gcatcaatct tcacattgac cttccatcat taacgcgctc tgaaaattga
    37321 gagcgaccaa atagaccgca taattaataa gccattttta tagccgctaa gatattaaag
    37381 gatgtgtcaa agatgcatac cccgatcggg gtaaaacctg tagcaggatc aaaagagtgg
    37441 cgggaagcgt ggcaaaaacg ggcttttgct cacatttcaa atggttataa acatatttat
    37501 atagcgatta attcaccaga gatatttctg ctggtttgct ccctcatcag aatttaacac
    37561 taaaagagca ggtaaaattg tctgaatgtt ctttaagtta ttcataaagc aaattaataa
    37621 atctgatgaa tatgttaacc ttcagcgaca tcatcggtga aaacctataa atgaagaagg
    37681 aaagcaaaaa aatgaagacc attctacctg aagtattgtt tgccgctttc gctaccactt
    37741 ccgcctgggc ggcagaaagc gtccagccac tggaaaaaat cgcgccttat ccacaagctg
    37801 aaacaggcat gaagcgtcag gtgattcagt taaccccgca agaagatgaa tctaccctga
    37861 aagtagaact gttaatcggt cagacgctgg aagtcgattg caatttgcat cgtctcggcg
    37921 ggaagctgga aagcaaaacg ctggaaggct ggggctatga ttattatgtc tttgataaag
    37981 ttagctcgcc ggtttccacg atgatggcct gcccggatgg caagaaagag aagaaatttg
    38041 tcaccgcgta tctgggcgat gctggaatgc tgcgttacaa cagcaagctg ccgatcgtgg
    38101 tgtatacgcc agacaatgta gatgtgaaat accgcgtctg gaaggcggaa gagaaaattg
    38161 acaacgcgga agttcgctaa actgttgtga agtacggcac cccgttggtt ggataaggcg
    38221 gttacgccgc atccaacatc taacgcccga gccggttgcc tgatgcgacg ctggcgcgtc
    38281 ttatcagtat acgccacatc attacaacgc aatatccgcc acttcttttt gaacgggttg
    38341 cggtttcaac tgcggtttcg gcgtactatc tgctgcttgc ggtttgtcgt atttcagccc
    38401 cagcacttcg ctggtgtact gcaactcgcg ttctgttgcc gctacatcac cgttcagctt
    38461 gcgtccataa gacggaacga tcgctttcaa cgtagcctgc cattgcgggc tggaaacgcg
    38521 atcgccaaat actttttcca gtagattcaa cataatcggc gcggcggttg acgcccctgg
    38581 cgatgccccc agcagcgccg caatggtccc ttgctggtca ctgaccactt cggtacccag
    38641 acgcagtacg ccacctttct cggcatcacg cttgataatc tgcacacgct gccccgcttg
    38701 ccacaaacgc cagtcctctt ttttcgcttg cggatagtac tctttcaacg cttcaaaacg
    38761 atcctcttca ctcaacatca cctgactcac cagatatttc accagatcga aattatccag
    38821 cccgacgtgc atcatcggca tcacgttaga ggtggtggtg gaactcatca gatcccacaa
    38881 tgaaccgttt ttgaggaatt tggttgagaa ggtggcaaat ggcccaaaca gcactacgcg
    38941 tttaccgtcc agaacacggg tatcgatatg cggaaccgac atcggtggtg cgccaacgga
    39001 tgctttaccg taaactttcg ccagatggcg attaaccacg tctggatttt ccgaaacaag
    39061 gaactgtccg cccaccggga aaccggcgta gtctttcgct tccggaatcc ccgattcctg
    39121 taacagcttc agcgccgcac cgcccgcgcc gataaagaca aatttggcgc ggatgttctg
    39181 tgcagtgccg tttttcagat cggcaacggt aacggtccag gtgttatcgt cattacgctt
    39241 tagggcgcgg acttcgctgc tgagttgcag cgagaagtta gatttcttct gtaaggaagc
    39301 aattaactgg cgggtgatct caccatagtt cacatcagtg ccaatttctg ttgcggatcg
    39361 cgcccttcca tcactaacgg tgcccactct ttgatctgcg cgtgatcttc agagtaacgc
    39421 ataccgcgaa acagcgagct ttgttgcaac gcggcgtaac gggcgcgcag gaaattaacg
    39481 ttatcctcgc cccagacaaa gctcatatgc ggaacggtat tgataaatga acgcggagta
    39541 cgcagcacgc cgcgctcgac ctggtgcgcc cagaactggc gggaaatctg aaatgcttcg
    39601 ttaatggcga ctgctttttc aatactgatg ctgccatcgg cgttttgcgg ggtgtagttc
    39661 agttccatca gtgcagaatg cccggttccg gcgttgttct agccgttcga actctcctgc
    39721 gcgcccccct ccaggcgctc caccatggtc atcgaccatt caggctccag ctcgcgtcaa
    39781 taggtcccca acgtggcgct cataatgccg ccgccaatca acagtacatc agtttcctgc
    39841 tcctcggacg ctttcgcttt tgccgccatc gaaacggcat taagccccac ggccatcgag
    39901 aagagcatgg cagtcacttt tttcatcttg ttaatgcctt acttttagtc gctttattgc
    39961 aggtgagatt tgcgcggcat caacggtaac acttaagtaa caacatttaa ataatgttta
    40021 taaattatat tctaatttta gaaacattat aatttcgttg attaattata gggttattag
    40081 aaatgaaggg atttttagca tattgacctg acggcagcag tccgccaggt cagcgtcgta
    40141 atattatgcc gtccgggcaa cggcatcacg cgaagcggca tcgcgctctt cgcccgtcag
    40201 ctcgctaagt tgcccattgc gcatttccag caggcggtcg gcgtggataa agtaatgatc
    40261 atcatgactg atagcgaaaa tagttttacc catctcctgc atcagcggca gcaacacctg
    40321 ataaaactca cgacggaagt gtggatcctg atccaccgcc cattcatcca gcaggataat
    40381 atcgcgttct tctgccagcg ccagcaacag cgccacgcgt tttttctgcc cttttgataa
    40441 cttcaggtta acaatacgcc cgttgcttaa ctcaagctta tgagccattt tcagctgcgc
    40501 cagccacttc tcaaccagtt gcgggttagc gggttgcccc tccggcccca gcagttgatc
    40561 aaacagccag acatcggtaa acactgccga aaacagtttg cgataatctt ccggttgttc
    40621 gccgctgaca ggtttgccat ccagcaagat ttcgccgctt tgtggctgat acaagcccgt
    40681 caacaacatc gccagcgtcg attttccgct accgttaccg ccaatcagaa acagcagctc
    40741 gccacgtttg atggtgagat taatcggacc aacggaaaac gcgttatcct gataagcaaa
    40801 cgtcacgtta cgcagctcca gcgtttgcca gttgggaaac gcctgcgggc gcggaaactc
    40861 agctttgaaa ggcgcgagcg cgaatttgtt cagcttgtta aacgccacct gcgccgtcag
    40921 cagcgtcggc aatgcgccaa ccgccgaaag cagcggcgta cgcaggaata acagcgtcaa
    40981 cgaataggtc gcagcaacgt tggtatcagc ccaaccgaga ctgttcgcca tccagaacac
    41041 caggccgata gcgcccagca tcatgatgtt tgaccagttc acggcactaa gatggaaggt
    41101 gtctgcgcga ataatatgat ggcgatactc ttgcgcatcg gggatgtaga ggttgttaaa
    41161 cacatactcg gcgcgttccc ggttcagagt cagctctttg cgcccttcaa gtacagtttg
    41221 aaaatccgtg tacagcttgt cttcggtttc acgtagggtc gccatatgtt tgtacacccg
    41281 cgccaccagc acaaaaccgc cccagatggt gatcgccatc cagatagccg ttaccagcaa
    41341 cattttgccc gacagcatcc acagatacgc cgatgaaccg atagtgagaa tgatcccctg
    41401 ccccagttcc ggcagacgca caaaagcaat ggtgatattg cgcacatcgc tggttaatcc
    41461 cgccagcaac gaggcgctac cgagttgttc aatgcgctcg acgtgggtat ccagaatccg
    41521 cttgataaat tcactacgca gtcggtaaac gaagtgatgc cccaaagtgg tgagcgccag
    41581 ttgcgatccg agagtgactg ccatcaacag cagcaataat cccagaaact ccggtaacac
    41641 cagcagactg gtatccgccg tttcgataag gcgctgattg ataaaagcaa ttaagccaat
    41701 gcctaatgcc gcactggcga ggcttagcgc catcacactg ataaatggcc agcgatactg
    41761 ccgccagaca agtacaagaa gttccatgca gaaaacccgg acaatgaatt acagcccgca
    41821 gtttaaacat cttcgcgcgc acagcaataa taattcttat ttttattctt ttttacctgc
    41881 ctgacggaat gtcaggttgt agcggcagtc ggtggtgagt ggatgaaacc ccgctttcaa
    41941 cggttgaata ccgtgataaa acagccgcga ttcaccgccc cataccacca catcgccatg
    42001 ttccaacaac aaacgtttga gcggatcatt acgtttcagg ccaccaaatt gaaaaatcgc
    42061 gggtaagccc agagaaacag aaacaattgg cgcgcgcaga tccggttcgt ctttatcctg
    42121 atgcagcgac agtttcgcgc caggagcgta gcggttgata agacaagcat ctggctggaa
    42181 atctggatag cccgccgccg tagccgcacg ttgacataaa tcatgaaagc tttgtggcat
    42241 agcgggccac ggtttatttg tttgcggatc aatgggcgaa tagagataac cttgccgatg
    42301 ggtcgtccag cccagatgcc cacagttggt catcgccacc gacatggtat atccgcctgg
    42361 agcgaccatc tggcgaaacg gcgactggct ggcaacgtca ttaatatcgc ggattagttg
    42421 ctccgcagca ttaaaagcaa aacgccgtaa aattaccgca ccagccgcca gtggctcttg
    42481 ccacggttca gcatcggcaa acagatccaa cattacctct cctcattttc agcttcgcgg
    42541 cgcagcagtt gcgctttacg cgacacgccc cagcggtaac cggaaagtgt gccatcacca
    42601 cggaccaccc gatgacaggg tattacgata gccagcttgt tggcggcaca ggcgctggca
    42661 acggcccgta ccgctttcgg tttgccgatg gcgttagcca gttgctgata actgacggtt
    42721 tcaccacaag gtatcgtgcg cagtgcctgc cagacttgtt gctgaaaagc agtgccgcga
    42781 atatccagcg gtaacgtcag cggcgtatcg cgttgattga ggctggcgat cacttcacgc
    42841 acatgttgct gaaacgtcag atcggcaggc gcgttgtcgg cagcgggaaa catctgctgc
    42901 aactcgctga ttaatgtcgc gtcatcatcg cccagcaata tcgcgcaaat cccccgctcg
    42961 ctttctgcca ccaggcaacg acccagctca caatcagcca gcgcgtaacg caccgccaga
    43021 ttttcgccac cgtgacggaa ttgtttagcc gtcatgccca gcgtttcatc ggctttgcgg
    43081 tagtaactgc tgctgtcggg gaatccggcg ttaagaatag acgtcgtcac gctctcccct
    43141 ttcgccagcg attcgcgcaa acggcgagcg cgccaggcct gttgccaggc tttaggcgtc
    43201 attccggtag tagctttaaa caaccgatgt agatgaaatg gactcatcgc cacctggtcg
    43261 gctaaggctt ccagcgttac aggcgtttcc tgttccagca gtcgacacgc gtgggtgatt
    43321 ttatccaacc gatgttgccg gggattggct ttgtctggct gacaacgttt gcaggggcga
    43381 aatccggcgg cgagtgcctc gctggcattt gcgtagaagg agacattttc ccgcaaggca
    43441 tgtctggcgc ggcaagaagg acggcaaaag atacccgtgg tgcgcacggc gaaaacgaat
    43501 tcgccgtcgg cattcgggtc gcgggctaag acagattgcc agcgttgatc gtcagttaag
    43561 catgtggcgt ttttcataat cagctccctg gttaaggata gcctttagtc tgcccggtca
    43621 ccatcacgca aaaaccaaca atcttgcgct ttaatttttt tcgctgacaa ggaagctttt
    43681 aaactgtggt gacatccagg ttttaaagct atcgccttct ttggtgatca tataaaccgc
    43741 cagcccttcc cggcgaacaa cctctttggc tttctccggc ccgagtacca tcaagccagt
    43801 atcccaggca tcggcttcca gcgccgtcgg agcaatcacc gtcacggata ccagattgtg
    43861 ttcgatggga cgcccggttt gcggatcgat aacatgggaa agacgtttgc cgtccagttc
    43921 gtaatagtta cggtagctgc cagaggtgct aatcccatga ccgttgatat ccaccacagc
    43981 ctgaaccgcg ttttctttat cggttggttt ttgaatcgct acccgccacg gctggccttc
    44041 accgttcata ccacggctgt tcagcgcgcc gcccaccgac accagatagc gggaaatccc
    44101 ttcctgctcc atcaagcgtg ccaggtgatc cgccgcataa ccttcaccga cggtggagag
    44161 atcgacatat aaatccggca ggtctttttg cagatattgc tgatgcgact gattgatgac
    44221 cgtcaggtgc tgtaagccgg ttttggcttt catcgcatcg atctgttcct ggctcggaat
    44281 ttgaaccggc tgttgttccg ggccaaagcc ccacagattc accagcggcc cgacggtaat
    44341 atccatcgca ccatcggtcc ttgccccaat gcgcagcgag gtggtgacga tatcggccat
    44401 cgcttcactt accggccacg gcgacaaact ttgcgagtcg ttaaagcgca tcagcgcgga
    44461 atctttttta taggtcgaaa gcagctgatc gtcggcgtcc agctgggtct gaatcttttc
    44521 tttaagttcg gcactgcgtt tggcatcaat gcccgggatg ctggcacgcc agaaggtacc
    44581 catggttttg ccttcaagaa cggtaacttc agtagcgtgg gttttggcgg gttgtggttt
    44641 ttgatcgcaa ccaacaaaga agagcgcggc aaccagcagt gccacgcggg taaagcttat
    44701 ttccatacgt gattatcctc atgcgaacgg tcgcaagagt acaccaaaaa actgtgtttg
    44761 tacgctgaaa acaatgaaaa aagggcccgc aggccctttg ttcgatatcc atcgagatta
    44821 gaactggtaa accagaccca gagctacgat gttatcagtg ttgatgccag cgtcacgagt
    44881 gaactggttg tcgtccagca ggttgatttt gtagtcaacg taggtggaca tgtttttgtt
    44941 gaagtagtag gtcgcaccaa catcaacata tttcaggata tcttcgtcgt cgtagttacg
    45001 accattgatg acacccaggt ttttaccttt agactgcagg taagccaggg acggacgcag
    45061 accgaagtcg aactggtact gagcaacagc ttcgaagttc tgtgctttgt tcgcccaacc
    45121 caagttgcct acgcgagttg cgttgtaggt ctgggtgtac tgagcagcta ggtagatgtt
    45181 gttcgcgtcg tatttcagac caccggtgta ggtttcagcg cggtcgccat taccgatgta
    45241 acgctcatca taacggttca gaccgaagtt ctggtcatca gtacgtttgg agctggaaac
    45301 tgcagcaccg ataccgaagc cttcgtaatc ataagtaata gaaccgccaa cgccgtcacc
    45361 gttctgacgc agagcttcac gaccgttgtt ggtcatgcct tcaccttccg ggctaccgtt
    45421 tttgccctga tactgaacag cgaagttcag accgtcaacc agaccaaaga agtcggtgct
    45481 acggtaggtt gcgaagccgt taccacgctg ctgcatgaag ttgtcagaac cgtaagtgtc
    45541 accaccgaat tctggcagta cgtcggtcca ggaagttacg tcgtaaacta cgccgtagtt
    45601 acgaccgtag tcgaaagaac ctacatcctg gaatttcaga cctgcgaatg ccacacgggt
    45661 ccaggagttg ttttcgtttt cagcgctgtt gccctggatc tgatattccc actggccgta
    45721 accggtcagc tggtcagtaa cctgagtttc acctttgaag ccaagacgca tgtaggtctg
    45781 gtcgccatct acagacttgt cgtcagagaa atagtgcagg ccgtctactt taccgtacag
    45841 atctaatttg ttgccgtctt tgttgtaaac ttcagcagcg tttgctgcgc ctgctaccag
    45901 cagagctggg accaggaggg acagtacttt aactttcatg ttattaaccc tctgttatat
    45961 gcctttattt gcttttttat gccactgcat actgattaac cctcattaat cagtcggcaa
    46021 gtccattctc cccaaaaatg cagaataatc caacacgaat atgatactaa aacttttaag
    46081 atgtttcatt tatcgctata gacgtttcaa aatgtaaata caagggaact ttttaagatt
    46141 attgcggaat ggcgaaataa gcacctaaca tcaagcaata ataattcaag gttaaaatca
    46201 ataacttatt cttaagtatt tgacagcact gaatgtcaaa acaaaacctt cactcgcaac
    46261 tagaataact cccgctatca tcattaactt tatttattac cgtcattcat ttctgaatgt
    46321 ctgtttaccc ctatttcaac cggatgcctc gcattcggtt tttttaccct tctttacaca
    46381 cttttcatta ttctgtgcta ccacagaaaa actataacgc ttgttaacta tttcacaaat
    46441 aattaacatc agcataattt ccagcaatct ttgtttattt gcaattaatt tcgttgggct
    46501 ttttgtaggt tatttgtaca gcaaaatggc gcttgtacat ctatttcccc ccatacagga
    46561 tgataaatat cacgggagaa tagagaatca tcaatcaggt aagagtctgg aatttcacac
    46621 tgtacccttt atactgccct atcacttcgc gaagttttaa caggtcataa acacgaatgc
    46681 gtcagaaaga gacaacggcc acgacccgct tttcactcct accggggagc attacccgct
    46741 tctttttact gttgatcatt gtgttactgg tgacgatggg ggtaatggta caaagtgccg
    46801 ttaacgcttg gctgaaagat aaaagttacc agattgtcga cattacccac gcaatccaaa
    46861 agcgcgtcga tacctggcgt tacgtgacca ggcagatcta cgacaacatt gccgcgacga
    46921 cctccccctc ctccggcgaa ggtttacaag agacgcgcct gaaacaggat gtctactatc
    46981 tggagaaacc acgccgcaaa acggaagcgt taatctttgg ctctcacgac aactcaacgc
    47041 ttgagatgac tcagcggatg tccacttatc tggatacatt gtggggtgca gaaaatgtac
    47101 cgtggtcgat gtattacctg aatggtcagg ataacagtct ggtgctgatc tcaaccctac
    47161 cccttaaaga tctcacctcc ggatttaaag aatcgaccgt cagtgacatt gttgattcac
    47221 gtcgtgcaga aatgttacaa caggccaacg ccctcgatga acgcgaaagt ttttctaaca
    47281 tgcgccgcct agcctggcag aacggtcatt actttacctt gcgtaccacg ttcaaccagc
    47341 cgggacatct ggcaacggtc gtggcttttg atctgccgat taatgatttg atcccaccgg
    47401 gtatgccgct ggacagtttc cgccttgagc cagacgcgac ggcaacggga aacaatgata
    47461 atgagaaaga agggacggat agcgtcagta tccactttaa cagtacgaag attgaaatct
    47521 cctcggcact caactctacc gatatgcgcc tggtctggca ggttccttat ggcaccttat
    47581 tgctggatac gttgcaaaac attctgctgc cactgctgct gaacatcggt ttgctggcgc
    47641 tggcgttatt tggctatacc acattccgcc atttctccag ccgcagtaca gaaagcgtcc
    47701 ccagcacggc ggtcaataac gaattgcgca ttttacgggc aatcaatgaa gagatagtct
    47761 cactgttgcc gctcggcctg ctggttcacg atcaggaatc gaaccgcact gtcataagta
    47821 acaaaattgc cgatcatttg ctgccgcatc tgaatctgca aaacatcacc accatggcgg
    47881 aacagcatca ggggattatt caggcgacga tcaataacga gctgtatgag atccgcatgt
    47941 tccgcagcca ggtcgcgccg cgcacacaaa ttttcattat tcgcgatcag gatcgcgaag
    48001 tgctggtaaa caagaaactc aagcaggcgc agcgtctgta tgagaaaaac cagcaggggc
    48061 ggatgacctt tatgaaaaac attggcgatg cgctgaaaga acccgcacag tccctggcgg
    48121 agagtgcggc taaactcaac gccccggaaa gcaaacaact ggcgaatcag gcagatgtgc
    48181 tggtgcggct ggtcgaagaa atacagttag cgaacatgct tgcggatgat agctggaaaa
    48241 gtgagacggt gctgttctcc gtgcaggatt taattgatga agttgtgcct tcagtgttgc
    48301 ctgccatcaa gcgtaaaggt ctgcaactgc tgattaacaa tcatctgaaa gcacacgata
    48361 tgcgccgcgg cgatcgcgat gccttacgac gtattttgct gctactgatg caatatgccg
    48421 tgacctcaac gcaattggga aaaatcaccc ttgaggttga tcaggatgag tcctctgaag
    48481 accgcctgac attccgcatt ctggacacgg gagaaggcgt aagtattcat gaaatggata
    48541 atttgcactt cccgtttatc aaccagaccc aaaacgatcg ttatggaaag gcggacccgc
    48601 tggcattctg gctgagcgat caactggcac gtaaactggg cggtcattta aacatcaaaa
    48661 cgcgggatgg gcttggtaca cgctactctg tgcatatcaa aatgctcgcc gctgacccgg
    48721 aagttgaaga ggaagaagag cgtttactgg atgatgtctg cgtaatggtg gatgttactt
    48781 cggcagaaat tcggaatatt gtcactcgcc tgttagaaaa ttggggtgca acctgtatca
    48841 cacccgatga aagattaatt agtcaagatt atgatatctt tttaacggat aatccgtcta
    48901 atcttactgc ctctggcttg cttttaagcg atgatgagtc tggcgtacga gaaattgggc
    48961 ctggtcaatt gtgcgtcaac ttcaatatga gcaacgctat gcaggaagcg gtcttacaat
    49021 taattgaagt gcaactggca caggaagagg tgacagaatc gcctctgggc ggagatgaaa
    49081 atgcgcaact ccatgccagc ggctattatg cgctctttgt agacacagta ccggatgatg
    49141 ttaagaggct gtatactgaa gcagcaacca gtgactttgc tgcgttagcc caaacggctc
    49201 atcgtcttaa aggcgtattt gccatgctaa atctggtacc cggcaagcag ttatgtgaaa
    49261 cgctggaaca tctgattcgt gagaaggatg ttccaggaat agaaaaatac atcagcgaca
    49321 ttgacagtta tgtcaagagc ttgctgtagc aaggtagcct attacatgaa caatatgaac
    49381 gtaattattg ccgatgacca tccgatagtc ttgttcggta ttcgcaaatc acttgagcaa
    49441 attgagtggg tgaatgttgt cggcgaattt gaagactcta cagcactgat caacaacctg
    49501 ccgaaactgg atgcgcatgt gttgattacc gatctctcca tgcctggcga taagtacggc
    49561 gatggcatta ccttaatcaa gtacatcaag cgccatttcc caagcctgtc gatcattgtt
    49621 ctgactatga acaacaaccc ggcgattctt agtgcggtat tggatctgga tatcgaaggg
    49681 atcgtgctga aacaaggtgc accgaccgat ctgccgaaag ctctcgccgc gctgcagaaa
    49741 gggaagaaat ttaccccgga aagcgtttct cgcctgttgg aaaaaatcag tgctggtggt
    49801 tacggtgaca agcgtctctc gccaaaagag agtgaagttc tgcgcctgtt tgcggaaggc
    49861 ttcctagtga ccgagatcgc taaaaagctg aaccgcagta ttaaaaccat cagtagccag
    49921 aagaaatctg cgatgatgaa gctgggcgtc gagaacgata tcgccctgct gaattatctc
    49981 tcttctgtga ccttaagtcc ggcagataaa gactaatcat ctgtaggcca gataagacgc
    50041 gttagtgtct tatctggcat ttgcaccgat tgccggatgc ggcgtaaacg ccttatccgg
    50101 cctacgattc ccattatttc aacagattac attaaagtag gccagataag acgcgtcagt
    50161 gtcttatctg gcatttgcac tgaatgccgg atgcggcgta aacgccttat ccgtcctacg
    50221 aatcccgcga tttcctgacc ctctcggcat ataccgtcag cgtctgtttt atcacatcca
    50281 gcgttaccgg cttcgagagg cagctgtcca taccggactc cagacaccgc tgcttctctt
    50341 cagccaacgc attagcagtt actccgatta ccggcaacgt cagtcccaac tgacgaatgc
    50401 gttgcgtcaa gcggtaacca tccatatttg gcatgttgac gtcgctaagc acgatatcaa
    50461 tatgattctt actaagtaca ttaagcgcat cgacgccatc attcgcggtt ttacattgat
    50521 agcccaacga tcccaactga tctgccagca gacgtcggtt aatcggatga tcatccacga
    50581 ccagaatcat catatcgtcg ttatcgctga ccgctttgtc cgtcgacggc agagcgttag
    50641 caggatcgtc gctctccatc tcgatcaaat aaatacgcgc caacaatgcc ggtagctcat
    50701 gcggagcagc cacactgtgt acccactccc ctggcgcttt ctccagcgga ataccaatat
    50761 ggcgacgaca gaaggtcact accgctctgc cctgccattt tttactcact acctcgtcag
    50821 tgattaacac atcttcggga gtcggttcct gcccttcgta tgttgtaacg acgatgccgc
    50881 tgcgctgcaa actggtttcc aggaactgac agagcgacgc attgcggacc gccagccagc
    50941 agcgtttacc actcaaccct tccacgcctt ttttctgcgg gtactgagcg ccgtacaacg
    51001 gaatacgcac ggtaaactgg ctgcccattc ccggttctga atctaccgag atatcgccgt
    51061 ccatcatgct gatcagtttt tcacaaatcg ccagacccag accggtcccc tggaagtttc
    51121 gctgtacgcc cgttccaacc tggaagaagg gatcaaacaa gcgcaccact tctttcgccg
    51181 gaatccccac gccggtatcg cgaacacgga tagagagata atcgccatca gcgcgaacat
    51241 gcaaaactat acagccggta tcggtgaatt ttatggcgtt actcaacagg ttggagatga
    51301 cctgctgtaa acgcatcggg tcgccattta aggccactgg cacatccggt tcaataaagc
    51361 agtacaagcc taactgcttg cgtaccacca gcggtaaata gttggcggtg atgtggttca
    51421 tcacttcacg cggtgaaaac tcacgcggtt cgatcttcaa ctgttccgat tcaatcttcg
    51481 agaaatcgag aatatcgctg ataattttca acaacaggct ggaagagttg ttcattgccg
    51541 tcaccagacg atcgacgcct ttcggtaact ctttggtttg caacagatcc aggttaccga
    51601 taatgccata cagcggcgtt cgcagctcat gactgacggt ggcaaggaac atcgattttg
    51661 actggctcgc ctgttccgct gcttgtgcca tctcctgcaa cgactcttcc atcttcacgc
    51721 gcgaagaaac atccaccagc acacaaatgg ccacgttttc attacgatag cgcgaatgga
    51781 cgaagctgat ttgcagattg gtattgttgc tggtcaggac atcaacaaaa ttgacctgct
    51841 gcccacagat aatttgcgtc agtcgttggc ggtcctcatg cgtaagcata ttgagatagg
    51901 tatgcgccag ttcgttactt aaaatattga cgccatcagc ggtacgcaaa atgcagatac
    51961 ccactggcgc ggaggcgaca atcttgcgat tgaactgctc atgttcttcc agtcgcaggg
    52021 cgtcgctttc cgccggaatg aaaatacgtc gctcgtacat ccgcgcgaga gtaaacaatg
    52081 cagctccggc aagcacattc agcaaaattg cgttaaggat caacatgcga atgcgttcca
    52141 gcaccttatc aaccggcacc gaatacacga tgcttagcga tgagggtggc agatttttct
    52201 tcagcaccag ctcccggaac ccttccgtat agccaaacca ggagcgttcc tgcatccagc
    52261 gaggatcgcc cttaatttta ctttctggtc cggtaagcga aatcagggta tgaccatttt
    52321 catcaagaat ggtaaccccc atcggcaacg tacccggtaa gaaaaagttc tccatccgga
    52381 tggtctgctc gacacccaaa agcgcctgca accggttcgc cagataaact ggcgtcaacg
    52441 cgtaaaaata cccgacgcaa gggcgcggac cttcgctgat ccagtagagg ttactgccgc
    52501 tatcatcttg tggtgcattt cgatatttat tgatgcgttc atgcaacgct ttcaacgcgg
    52561 tatcgcgttc cactggcata tcacgcagac cgaaattggc catgcagagg ttatcactgc
    52621 cgattaaaaa tacccggttg agatcgtaag ccgcagaaaa attatcgcgc cagtagcgca
    52681 taaaccacgc caatgactcc agagaacctc gccaggtgtt actcattgcg gaacaatcgg
    52741 agtcggcaaa cagcggttca aacgcaggca catccgcctg cgtttctcgt ccacgcgggg
    52801 aaagcacacc gttttctgcc gataagcgat tttcggcgat gtacttaagc tctttcatca
    52861 catcagaggt gcgttgaata aagcgctgag cctgatcgga actcagatta aattcctgac
    52921 gaatttccga ttctcgctga tgtaacgcat taacgatgta aaaaacggat gaaaaagcaa
    52981 tcaacagcca gagcactaac gccaatgctc tgaacatgta gcgcgaggct ttcagggttg
    53041 tacgaaaaga agcaaggtat ttcaaagggg agaagctccg cctcaggtga ccgatggagt
    53101 gtggttaagg tagcggtaaa agcgtgttac cgcaatgttc tgtcaccatt gcaataatgc
    53161 gtatcgctta gaaaccattg ccttaaccag gaaagccgga gtagatggac agttctcagt
    53221 acgaaaaata agaaatgacc aatctgccga cgcttgttaa tggttatatc ctggcgtcgg
    53281 cagatcgccc agcagtctta tctgaaaaaa gagagagcca ggttgtgtga agggatggat
    53341 caccagtcaa aacttttcac cagcgtcagc tcgccagcat tacgcatcgg tacaataaat
    53401 gtttcctgtt tctcattgac cgatccttca tcggtgatca gcgtcagttg ggcggtcgtc
    53461 aattccgttt cgctgcgccc accatagtag ttgatataca cctgatagcg cccgtgaact
    53521 ggtgctggca tggcgaaaat ctcgggtccg taccccgtcg tgacatccat atccagtgca
    53581 ccactgtttt tcagcacggt gttaccgtac caggcgtgtt cgccatcggg cgtgacaaca
    53641 tggagatcga gatcggtatt gtccgtatcc cacgagagaa ccagccgtaa acgtgcacga
    53701 atagttcctg tacccggcgt tgagtaaaac tgcatttttt gccggctttg cccatccggg
    53761 ctgatgacct gcacgctgtt gctgccttca gtgaaaatat aagggcgtgc aaatgaacca
    53821 tcggattcaa ttctctgcgg catactggca ccgttgacca ccagccggcc ctgctgaact
    53881 ttacccgccg cagcataatt ctttattttg ccgcggatct gcgctgagat attttgatca
    53941 tctgccatgt tgacggacga cgccgggtaa ttgatcgatt gtgaaaaact ggcatcttcg
    54001 ccctctgccg gatgccagcc agagagcggt gcgtcaatct cgacttcctg cacaccttca
    54061 ctgtgagcga caggcgaaag tgccaccagt aacaacggaa gaaaaatctt tcgcatagca
    54121 ttagtccatt aataattgtc gggtcagcgt ttcgatatag ttctcatcaa gcccggtggg
    54181 ataaccatca aaggccaagt gcagatactc atgggttaaa tccagccgat cctgaagcgt
    54241 aaagaagtta cgaataaaca gccgtttctg ctgccgatcg gtatagggga aacctgagac
    54301 taaacgacag accgcaaaca cgtctggttc gttgtacccc gtctcgcctt gtaatatacg
    54361 ccgccactgc ggcatttttt tcgccagcca ggctttcgct ttgggtaata actggcaggt
    54421 tgagcgtgga gctccccagc ggctaaggct gttgtcggga taagcaaacg cgagtatctg
    54481 gtcgtaacgc tccccctgcc ccgcctgggc cgtggcttgc cgccaggaaa gtgtcccttc
    54541 ggtagcgcga ctgccatgat aatgaaccgg atcgcctgca tagatgagct cctgcgtcca
    54601 ggcggtcatc gttcgcgccc ccgtggtcgc tggcgaagca gaaacacgct gcgtggcgct
    54661 gctgtcgggg atggtcaggc aatcaccttc ccgattggcg ttttgctgta aaaaagtacg
    54721 tatcgccaca gtcatggctt tcgctgcctc aggtggcgta cttttcgcct cgcgatccag
    54781 aactctggca acgtactctt cacgatcgag atgcgactgc aatttcagtt tgcccttttc
    54841 gctcagtaag gtcgtctcac cgtggctgac aaacgtaatg tggttaccat tagtaaacgt
    54901 cacacgatag cgcccattaa gtacgctggg ttttactgcc gtcgtactct tttccgctgt
    54961 gattttcttc agcggatagc gggcaaacag ctccacctct acacattgcc cactggcaac
    55021 ctgcgtcggc actggcaaaa cccgattaag caccgtcgca taacgggtta gtaccgtttt
    55081 gctggtgcca ctgccagtaa cccacagcgg tgtaccatcg gttaaccagc cagcaaaacc
    55141 accctgacgg atttcctgtt tatcatcggc aaaccagctc caggttttca cccgtacccg
    55201 actaccgagc atggatgcga caccgatttt cgcctcgtcc agcaccacat ccagtaagac
    55261 ttcctgagct ttgttttgcg caggtaacgt cgctaatgaa tcaagcagcg atttaaccgt
    55321 taccgaggtt tctggcttca gcgttgtaag agacgctaac cacgcaggtg cctgacgctg
    55381 ttgccagtat tgcccccaca catctgcgcc aatatgcaac cgctgcggcg caaaatagag
    55441 cccacaggaa cgtaccagcg cggtatcgcg cgttatgctc tccccagcct gacaacaata
    55501 aacctcttcc ggggagtttc cgcgacactg gtagggctgc tccggttgat gggtatcctc
    55561 cagccaggcg taaacataca gtttccacaa actccctaac ggcgtggtga gcgagtcagg
    55621 taatgctgag actttggtca cgcctgatga cgacagctga tacagttgat cgtgctgcgc
    55681 accgcgcagc accagttgta acggcgtctc ctccgcaagc gttggcagag taaccaacgc
    55741 caatagccag acgattctgc gccagttcac ttatttcacc tgcatcccgg tccattcact
    55801 ccctgccgcg acactttgct gcgcaggtgc ataggaacgc acataacgag caggaggcag
    55861 aacgaattgc cctttttgcg agaagcgcag caaatggcgg aaagtgaccg ttcccgtcag
    55921 ttctttcacc ggcaccatat aggccagttc gcccatttca ttacgcgctt tttccagcaa
    55981 ttgcccctgc tgtttcgcgg cgttgggttt attgactgaa atgccccatg ttgtgcgctc
    56041 aacgtcagct cccggcggga gcggtacttc cacctgacca tagcgcagaa ctgcatcctg
    56101 ctcgctggta agcgtaattt catcgagata cagcgcatcg ctgtcaatct cattgctggt
    56161 caccggctgc agagtaaagc tcatctcttc ttcaccgggg ataagccgat acaactggcg
    56221 ttcaacggtc accggaatgt tactttgttg agccgttttt gccggttcac gccagcggac
    56281 ctgcacattt gcggcgataa ttcgtcacca aaagagagaa tgtccggcac accctaacca
    56341 acccaacgcc agtcttcgcc ccctccagtt aacttatgtt tagcccatgc gcccgcaggc
    56401 gcaggcaaaa caactggagg cattgtcgcc atatatttcg ccagccagtt catggccagc
    56461 gcgcgctcaa tagtggattg ctcagcggtc aaaccattaa aatagcggcc gtctgggtag
    56521 cgtcaccacc gctattgagc agcacaaccg tgtgcgccag cggctgattg ctgtttgccg
    56581 ctttatccca cgcatattgc tgaacctgtg gcatcaccgt ggacttcaac tgcgcttttt
    56641 tcagcaacgt catggttaag acgtttgcca ccgcatctgc cagtggagat tccggtgtat
    56701 cgaggatcag gctgtcattg atatcgcgga tgtcttcctc agagaaatct tcatctttag
    56761 ttccgcgccg ggcgatagct tcatcccccc ctttcaacaa cgttttgcac ggcagattca
    56821 tctcctgtgc ccacgccagc accagcgccc gatgcaataa cggcatatta tccgcctgct
    56881 cggcataact gtcgagcata tgctgccagt attccggttg ttgcgttacg ccgagcgcct
    56941 ggctggcctg ccagtcggcg taccatgccc atgccgtaag gaaggcgtca ccattgccat
    57001 cttcacccca ccaggtaaag cgcgctccgg gccccgccag ttgcatcagc cgcagacggt
    57061 tatcctgaat catctgacga atgtcgttag cggcggcact ttgatgatcg gcaagcgaac
    57121 gccaggcgag actgagcggg atcagacggc taccggtgtt gatcacccca ccccacggtt
    57181 catcgactaa cgcatcaaga ttgttgcgga aaatctcctg cggcgtttca ctactttgta
    57241 gccggatatt actcgcctgc tcgggcaaca tcagcgcgtt ctcgccaccg ccgagcatga
    57301 catttttctg ctgttcaacg ggccagctgt tgtccacaaa agacagtttt gtgctaatgc
    57361 tgtcctgcac ttgcccattt tgttgcagtt ctgcacttaa caagccagat tgctgaatat
    57421 tctgcgtcag cgaaatataa ttcgcccctt tgtgcagcgt cagcgtctgg cgcatctcag
    57481 cgcctgcaaa tttagtcacc agcgccaccg gttcgttatc ctgctgactg aagataaaca
    57541 gtcctgccgc cggtttgtcg cccacgcgat acaccgttgg catactccac ttcatgtaga
    57601 gatttttttc cgaacgcaga taagcacgcc cctgcccgac cagcccgtcg ccgttcatcc
    57661 cacgcgcggt gatacgccag cgggttaacg aatcaggcat caggaacgtg aaatacgctt
    57721 tgccttgttt atcggttgtg agtgacggca tccatgccgc ggtatccacc tcttcacgcc
    57781 gtggacgttc aagcattttt actcgccgct cgctgcggtt agtcgcgcca ggcgcaaccg
    57841 gctcgctgga gagcgcctgg tcatagctga taaacgacag actggagctg gtacgcacat
    57901 tgttacgccc cagcggatag aaaaatttgc cgatattcgg cgcgatttct ggttgcagcg
    57961 cgtagatcat ttcatcgacc acgcctaccg ttagctgcgc agaaacaggt ttacctttca
    58021 gcgacgaggt taatt
//
LOCUS       DAJTRC010000015        55420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.015, whole genome shotgun sequence.
ACCESSION   DAJTRC010000015 DAJTRC010000000
VERSION     DAJTRC010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 55420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 55420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.015"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO357_000995"
     CDS             complement(113..1486)
                     /gene="argH"
                     /locus_tag="OO357_000995"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HCR5886027.1"
                     /translation="MALWGGRFTQAADQRFKQFNDSLRFDYRLAEQDIVGSVAWSKAL
                     VTVGVLTAEEQAQLEEALNVLLEDVRARPQQILESDAEDIHSWVEGKLIDKVGQLGKK
                     LHTGRSRNDQVATDLKLWCKDTVSELLTANRQLQSALVETAQNNQDAVMPGYTHLQRA
                     QPVTFAHWCLAYVEMLARDESRLQDALKRLDVSPLGCGALAGTAYEIDREQLAGWLGF
                     ASATRNSLDSVSDRDHVLELLSAAAIGMVHLSRFAEDLIFFNTGEAGFVELSDRVTSG
                     SSLMPQKKNPDALELIRGKCGRVQGALTGMMMTLKGLPLAYNKDMQEDKEGLFDALDT
                     WLDCLHMAALVLDGIQVKRPRCQEAAQQGYANATELADYLVAKGVPFREAHHIVGEAV
                     VEAIRQGKPLEDLPLSELQKFSQVIGEDVYPILSLQSCLEKRAAKGGVSPQQVAQAIA
                     FAQARLE"
     gene            complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO357_000996"
     CDS             complement(1547..2323)
                     /gene="argB"
                     /locus_tag="OO357_000996"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418394.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HCR5886028.1"
                     /translation="MMNPLIIKLGGVLLDSEEALERLFSALVNYRESHQRPLVIVHGG
                     GCVVDELMKGLNLPVKKKNGLRVTPADQIDIITGALAGTANKTLLAWAKKHQIAAVGL
                     FLGDGDSVKVTQLDEELGHVGLAQPGSPKLINSLLENGYLPVVSSIGVTDEGQLMNVN
                     ADQAATALAATLGADLILLSDVSGILDGKGQRIAEMTAAKAEQLIEQGIITDGMIVKV
                     NAALDAARTLGRPVDIASWRYAEQLPALFNGMPMGTRILA"
     gene            complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO357_000997"
     CDS             complement(2331..3335)
                     /gene="argC"
                     /locus_tag="OO357_000997"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HCR5886029.1"
                     /translation="MLNTLIVGASGYAGAELVTYVNRHPHMNITALTVSAQSNDAGKL
                     ISDLHPQLKGIVELPLQPMSDISEFSPGVDVVFLATAHEVSHDLAPQFLEAGCVVFDL
                     SGAFRVNDATFYEKYYGFTHQYPELLEQAAYGLAEWCGNKLKEANLIAVPGCYPTAAQ
                     LALKPLIDADLLDLNQWPVINATSGVSGAGRKAAISNSFCEVSLQPYGVFTHRHQPEI
                     ATHLGADVIFTPHLGNFPRGILETITCRLKSGVTQAQVAQALQQAYAHKPLVRLYDKG
                     VPALKNVVGLPFCDIGFAVQGEHLIIVATEDNLLKGAAAQAVQCANIRFGYAETQSLI
                     "
     gene            3489..4640
                     /gene="argE"
                     /locus_tag="OO357_000998"
     CDS             3489..4640
                     /gene="argE"
                     /locus_tag="OO357_000998"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HCR5886030.1"
                     /translation="MKNKLPPFIEIYRALIATPSISATEEALDQSNADLITLLADWFK
                     DLGFNVEVQPVPGTRNKFNMLASYGQGAGGLLLAGHTDTVPFDDGRWTRDPFTLTEHD
                     GKLYGLGTADMKGFFAFILDALRDVDVTKLKKPLYILATADEETSMAGARYFAETTAL
                     RPDCAIIGEPTSLQPVRAHKGHISNAIRIQGQSGHSSDPARGINAIELMHDAIGHILQ
                     LRDNLKERYHYEAFTVPYPTLNLGHIHGGDASNRICACCELHMDIRPQPGMTLNELNG
                     LLNDALAPVSERWPGRLTVDELHPPIPGYECPPNHQLVEVVEKLLGAKTEVVNYCTEA
                     PFIQTLCPTLVLGPGSINQAHQPDEYLETRFIKPTRELIIQVIHHFCWH"
     gene            4992..7643
                     /gene="ppc"
                     /locus_tag="OO357_000999"
     CDS             4992..7643
                     /gene="ppc"
                     /locus_tag="OO357_000999"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004203687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HCR5886031.1"
                     /translation="MNEQYSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKS
                     SRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPE
                     VIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQ
                     LDNKDIADYERNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVP
                     NYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKAT
                     DLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARL
                     KGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVR
                     IDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAE
                     TREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETL
                     DDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIK
                     TCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLP
                     EITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDLYRGYVRENKDFVPYF
                     RSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQK
                     VVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKEL
                     RNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKE
                     GQEPDPRVEQALMVTIAGIAAGMRNTG"
     gene            7826..9559
                     /locus_tag="OO357_001000"
     CDS             7826..9559
                     /locus_tag="OO357_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase CptA"
                     /protein_id="HCR5886032.1"
                     /translation="MHSTEVQAKPLSSWKALGWALLYFWFFSTLLQAIIYISGYSGTN
                     GIRDSLLFSSLWLIPVFLFPKRIKIIAAVIGVVLWTASLAALCYYVIYGQEFSQSVLF
                     VMFETNTNEASEYLSQYFSLKIVLIALAYTAVAVLLWTRLRPVYIPKPWRYVVSFALL
                     YGLILHPIAMNTFIKNKPFEKTLDNLASRMEPAAPWQFLTGYYQYRQQLNSLTKLLNE
                     NNALPPLANFKDESGNEPRTLVLVIGESTQRGRMSLYGYPRETTPELDALHKTDPNLT
                     VFNNVVTSRPYTIEILQQALTFANEKNPDLYLTQPSLMNMMKQAGYKTFWITNQQTMT
                     ARNTMLTVFSRQTDKQYYMNQQRTQSAREYDTNVLKPFQDVLNDPAPKKLIIVHLLGT
                     HIKYKYRYPENQGKFDGNTDHVPPGLSAEELESYNDYDNANLYNDHVVASLIKDFKAA
                     DPNGFLVYFSDHGEEVYDTPPHKTQGRNEDNPTRHMYTIPFLLWTSEKWQATHPRDFS
                     QDVDRKYSLAELIHTWSDLAGLSYDGYDPTRSVVNPQFKETTRWIGNPYKKNALIDYD
                     TLPYGDQVGNQ"
     gene            9708..10559
                     /locus_tag="OO357_001001"
     CDS             9708..10559
                     /locus_tag="OO357_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5886033.1"
                     /translation="MYHDVSYLLSRLINGPLSLRQIYFASSNGPVPDLAYQVDFPRLE
                     IVLEGEFVDTGAGATLVPGDVLYVAAGGWNFPQWKTPATTFSVLFGKQQLGFSVVQWD
                     GKQYQNLAKQHVARRGPRIGSFLLQTLNEMQMQPQEQQTARLIVASLLSHCRDLLGSQ
                     IQTASRSQALFEAIRDYIDERYASALTRESVAQAFYISPNYLSHLFQKTGAIGFNEYL
                     NHTRLEHAKTLLKGYDLKVKEVAHACGFVDSNYFCRLFRKNTEHSPSEYRRQYHSQLT
                     EKPTTPE"
     gene            complement(10546..10887)
                     /locus_tag="OO357_001002"
     CDS             complement(10546..10887)
                     /locus_tag="OO357_001002"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5886034.1"
                     /translation="MAYLVAVTACVSGVAHTYMAAERLEKLCQLEKWGVSIETQGALG
                     TENRLADEDIRRADVALLITDIELAGAERFEHCRYVQCSIYAFLREPQRVMSAVRKVL
                     SAPQQTHLILE"
     gene            complement(10889..11767)
                     /locus_tag="OO357_001003"
     CDS             complement(10889..11767)
                     /locus_tag="OO357_001003"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709752.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[formate-C-acetyltransferase]-activating enzyme"
                     /protein_id="HCR5886035.1"
                     /translation="MTSSAGQRISCNVVETRRDDVARIFNIQRYSLNDGEGIRTVVFF
                     KGCPHLCPWCANPESISGKIQTVRREAKCLHCAKCLRDADECPSGAFERIGRDISLDA
                     LEREVMKDDIFFRTSGGGVTLSGGEVLMQAEFATRFLQRLRLWGVSCAIETAGDAPAS
                     KLLPLAKLCDEVLFDLKIMDATQARDVVKMNLPRVLENLRLLVSEGVNVIPRLPLIPG
                     FTLSRENMQQALDVLTPLKIRQIHLLPFHQYGEPKYRLLGKTWSMKEVPAPSSADVTT
                     MREMAERAGFQVTVGG"
     gene            complement(11733..14030)
                     /locus_tag="OO357_001004"
     CDS             complement(11733..14030)
                     /locus_tag="OO357_001004"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5886036.1"
                     /translation="MTNRISRLKTALFANTREISLERALLYTASHRQTEGEPVILRRA
                     KATAYILEHVEISIRDEELIAGNRTVKPRAGIMSPEMDPYWLLKELDQFPTRPQDRFA
                     ISEEDKRIYREELFPYWEKRSMKDFINGQMTDEVKAATNTQIFSINQTDKGQGHIIID
                     YPRLLNHGLGELVAQMQQHCQQQPENHFYQAALLLLEASQKHILRYAELAETMAANCT
                     DAQRREELLTIAEISRHNAQHKPQTFWQACQLFWYMNIILQYESNASSLSLGRFDQYM
                     LPFYQASLTQGEDPAFLKELLESLWVKCNDIVLLRSTSSARYFAGFPTGYTALLGGLT
                     ENGRSAVNVLSFLCLDAYQSVQLPQPNLGVRTNALIDTPFLMKTAETIRLGTGIPQIF
                     NDEVVVPAFLNRGVSLEDARDYSVVGCVELSIPGRTYGLHDIAMFNLLKVMEICLHEN
                     EGNAALTYEGLLEQIRAKISHYITLMVEGSNICDIGHRDWAPVPLLSSFISDCLEKGR
                     DITDGGARYNFSGVQGIGIANLSDSLHALKGMVFEQQRLSFDELLSVLKANFATPEGE
                     KVRARLINRFEKYGNDIDEVDNISAELLRHYCKEVEKYQNPRGGYFTPGSYTVSAHVP
                     LGSVVGATPDGRFAGEQLADGGLSPMLGQDAQGPTAVLKSVSKLDNTLLSNGTLLNVK
                     FTPATLEGEAGLRKLADFLRVFTQLKLQHIQFNVVNADTLREAQQRPQDYAGLVVRVA
                     GYSAFFVELSKEIQDDIIRRTAHQL"
     gene            complement(14081..14401)
                     /locus_tag="OO357_001005"
     CDS             complement(14081..14401)
                     /locus_tag="OO357_001005"
                     /EC_number="2.7.1.202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIB"
                     /protein_id="HCR5886037.1"
                     /translation="MTKIIAVTACPSGVAHTYMAAEALESAAKAKGWEVKVETQGSIG
                     LENELTAEDVASADMVILTKDIGIKFEERFAGKTIVRVNISDAVKRADAIMSKIEAHL
                     AQTA"
     gene            complement(14416..15495)
                     /locus_tag="OO357_001006"
     CDS             complement(14416..15495)
                     /locus_tag="OO357_001006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit EIIC"
                     /protein_id="HCR5886038.1"
                     /translation="MNELVQILKNTRQHLMTGVSHMIPFVVSGGILLAVSVMLYGKGA
                     VPDAVADPNLKKLFDIGVAGLTLMVPFLAAYIGYSIAERSALAPCAIGAWVGNSFGAG
                     FFGALIAGIIGGIVVHYLKKIPVHKVLRSVMPIFIIPIIGTLITAGIMMWGLGEPVGA
                     LTNSLTQWLQGMQQGSIVMLAVIMGLMLAFDMGGPVNKVAYAFMLICVAQGVYTVVAI
                     AAVGICIPPLGMGLATLIGRKNFSAEERETGKAALVMGCVGVTEGAIPFAAADPLRVI
                     PSIMVGSVCGAVTAALVGAQCYAGWGGLIVLPVVEGKLGYIAAVAVGAVVTAVCVNVL
                     KSLARKNGSSTDEKEDDLDLDFEIN"
     gene            15804..16907
                     /locus_tag="OO357_001007"
     CDS             15804..16907
                     /locus_tag="OO357_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001185158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HCR5886039.1"
                     /translation="MALIVEFICELPNGVHARPASHVETLCNTFSSQIEWHNLRTDRK
                     GNAKSALALIGTDTLVGDNCQLLISGADEQEGHQRLSQWLRDEFPHCDAPLAEVKSDE
                     LEPLPVSLTNLNPQIIRARTVCSGSAGGILTPISSLDLNALSNLPAAKDVDAEQSALE
                     NGLTLVLKNIEFRLLDSDGATSAILEAHRSLAGDTSLHEHLLAGVSAGLSCAEAIVAS
                     ANHFCEEFARSSSRYLQERALDVRDVCFQLLQQIYGEQRFPAPGKLTQSAICMADELT
                     PSQFLELDKNHLKGLLLKSGGTTSHTVILARSFNIPTLVGVDIDALTPWQHQTIYIDG
                     NAGAIVVEPGEAVARYYQQEARVQDALREQQRV"
     gene            16908..18305
                     /gene="ptsP"
                     /locus_tag="OO357_001008"
     CDS             16908..18305
                     /gene="ptsP"
                     /locus_tag="OO357_001008"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5886040.1"
                     /translation="MTQQARTADGIRIEIAANIAHSVEAQAAFGNGAEGVGLFRTEML
                     YMDRTSAPGESELYNIFCQALESANGRSIIVRTMDIGGDKPVDYLNIPAEANPFLGYR
                     AVRIYEEYASLFTTQLRSILRASAHGSLKIMIPMISSMEEILWVKEKLAEAKQQLRNE
                     HIPFDEKIQLGIMLEVPSVMFIIDQCCEEIDFFSIGSNDLTQYLLAVDRDNAKVTRHY
                     NSLNPAFLRALDYDVQAVHRQGKWIGLCGELGAKGSVLPLLVGLGLDELSMSAPSIPA
                     AKARMAQLDSRECRKLLNQAMACRTSLEVEHLLAQFRMTQQDAPLVTAECITLESDWR
                     SKEEVLKGMTDNLLLAGRCRYPRKLEADLWAREAVFSTGLGFSFAIPHSKSEHIEQST
                     ISVARLQAPVRWGDDEAQFIIMLTLNKHAAGDQHMRIFSRLARRIMHEEFRNALVNAA
                     SADAIASLLQHELEL"
     gene            18317..18979
                     /gene="fsa"
                     /locus_tag="OO357_001009"
                     /pseudo
     CDS             18317..18979
                     /gene="fsa"
                     /locus_tag="OO357_001009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418381.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            18990..20091
                     /gene="gldA"
                     /locus_tag="OO357_001010"
                     /pseudo
     CDS             18990..20091
                     /gene="gldA"
                     /locus_tag="OO357_001010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418380.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional L-1,2-propanediol
                     dehydrogenase/glycerol dehydrogenase"
     gene            20366..20973
                     /locus_tag="OO357_001011"
                     /pseudo
     CDS             20366..20973
                     /locus_tag="OO357_001011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418379.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1287 domain-containing protein"
     gene            complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO357_001012"
     CDS             complement(21000..21905)
                     /gene="yijE"
                     /locus_tag="OO357_001012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine transporter YijE"
                     /protein_id="HCR5886041.1"
                     /translation="MSAAGKSNPLAISGLVVLTLIWSYSWIFMKQVTSYIGAFDFTAL
                     RCIFGALVLFIVLLLRGRGMRPTPFKYTLAIALLQTCGMVGLAQWALVSGGAGKVAIL
                     SYTMPFWVVIFAALFLGERLRRGQYFAILIAAFGLFLVLQPWQLDFSSMKSAMLAILS
                     GVSWGASAIVAKRLYARHPRVDLLSLTSWQMLYAALVMSVVALLVPQREIDWQPTVFW
                     ALAYSAILATALAWSLWLFVLKNLPASIASLSTLAVPVCGVLFSWWLLGENPGVVEGS
                     GIVLIVLALALVSRKKKEAVSVKSI"
     gene            complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO357_001013"
     CDS             complement(21999..24179)
                     /gene="katG"
                     /locus_tag="OO357_001013"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HCR5886042.1"
                     /translation="MSTSDDIHNTTATGKCPFHQGGHDQSAGAGTTTRDWWPNQLRVD
                     LLNQHSNRSNPLGEDFDYRKEFSKLDYYGLKKDLKALLTESQPWWPADWGSYAGLFIR
                     MAWHGAGTYRSIDGRGGAGRGQQRFAPLNSWPDNVSLDKARRLLWPIKQKYGQKISWA
                     DLFILAGNVALENSGFRTFGFGAGREDVWEPDLDVNWGDEKAWLTHRHPEALAKAPLG
                     ATEMGLIYVNPEGPDHSGEPLSAAAAIRATFGNMGMNDEETVALIAGGHTLGKTHGAG
                     PTSNVGPDPEAAPIEEQGLGWASTYGSGVGADAITSGLEVVWTQTPIQWSNYFFENLF
                     KYEWVQTRSPAGAIQFEAVDAPEIIPDPFDPSKKRKPTMLVTDLTLRFDPEFEKISRR
                     FLNDPQAFNEAFARAWFKLTHRDMGPKSRYIGPEVPKEDLIWQDPLPQPIYNPTEQDI
                     IDLKFAIADSGLSVSELVSVAWASASTFRGGDKRGGANGARLALMPQRDWDVNAAAVR
                     ALPVLEKIQKESGKASLADIIVLAGVVGVEKAASAAGLSIHVPFAPGRVDARQDQTDI
                     EMFELLEPIADGFRNYRARLDVSTTESLLIDKAQQLTLTAPEMTALVGGMRVLGANFD
                     GSKNGVFTDRVGVLSNDFFVNLLDMRYEWKATDESKELFEGRDRETGEVKYTASRADL
                     VFGSNSVLRAVAEVYASSDAHEKFVKDFVAAWVKVMNLDRFDLL"
     gene            complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO357_001014"
     CDS             complement(24508..25398)
                     /gene="metF"
                     /locus_tag="OO357_001014"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HCR5886043.1"
                     /translation="MSFFHASQRDALNQSLAEVQGQINVSFEFFPPRTSEMEQTLWNS
                     IDRLSSLKPKFVSVTYGANSGERDRTHSIIKGIKDRTGLEAAPHLTCIDATPDELRTI
                     ARDYWNNGIRHIVALRGDLPPGSGKPEMYASDLVTLLKEVADFDISVAAYPEVHPEAK
                     SAQADLLNLKRKVDAGANRAITQFFFDVESYLRFRDRCVSAGIDVEIIPGILPVSNFK
                     QAKKFADMTNVRIPAWMAQMFDGLDDDAETRKLVGANIAMDMVKILSREGVKDFHFYT
                     LNRAEMSYAICHTLGVRPGL"
     gene            complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO357_001015"
     CDS             complement(25747..28179)
                     /gene="metL"
                     /locus_tag="OO357_001015"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000110794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase II"
                     /protein_id="HCR5886044.1"
                     /translation="MSVIAQAGAKGRQLHKFGGSSLADVKCYLRVAGIMAEFSQPDDM
                     MVVSAAGSTTNQLINWLKLSQTDRLSAHQVQQTLRRYQCDLISGLLPAEEADSLISAF
                     VSDLERLAALLDSGINDAVYAEVVGHGEVWSARLMSAVLNQQGLPAAWLDAREFLRAE
                     RAAQPQVDEGLSYPLLQQLLVQHPSKRLVVTGFISRNNAGETVLLGRNGSDYSATQIG
                     ALAGVSRVTIWSDVAGVYSADPRKVKDACLLPLLRLDEASELARLAAPVLHARTLQPV
                     SGSEIDLQLRCSYTPDQGSTRIERVLASGTGARIVTSHDDVCLIEFQVPASQDFKLAH
                     KEIDQILKRAQVRPLAVGVHNDRQLLQFCYTSEVADSALKILDEAGLPGELRLRQGLA
                     LVAMVGAGVTRNPLHCHRFWQQLKGQPVEFTWQSDDGISLVAVLRTGPTESLIQGLHQ
                     SVFRAEKRIGLVLFGKGNIGSRWLELFAREQSTLSARTGFEFVLAGVVDSRRSLLSYD
                     GLDASRALAFFNDEAVEQDEESLFLWMRAHPYDDLVVLDVTASQQLADQYLDFASHGF
                     HVISANKLAGASDSNKYRQIHDAFEKTGRHWLYNATVGAGLPINHTVRDLIDSGDTIL
                     SISGIFSGTLSWLFLQFDGSVPFTELVDQAWQQGLTEPDPRDDLSGKDVMRKLVILAR
                     EAGYNIEPDQVRVESLVPAHCEGGSIDHFFENGDELNEQMVQRLEAAREMGLVLRYVA
                     RFDANGKARVGVEAVREDYPLASLLPCDNVFAIESRWYRDNPLVIRGPGAGRDVTAGA
                     IQSDINRLAQLL"
     gene            complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO357_001016"
     CDS             complement(28182..29342)
                     /gene="metB"
                     /locus_tag="OO357_001016"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HCR5886045.1"
                     /translation="MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFTGFNEPRAH
                     DYSRRGNPTRDVVQRALAELEGGAGAVLTNTGMSAIHLVTTVFLKPGDLLVAPHDCYG
                     GSYRLFDSLAKRGCYRVLFVDQGDEQALRAALAEKPKLVLVESPSNPLLRVVDIAKIC
                     HLAREVGAVSVVDNTFLSPALQNPLALGADLVLHSCTKYLNGHSDVVAGVVIAKDPDV
                     VTELAWWANNIGVTGGAFDSYLLLRGLRTLVPRMELAQRNAQAIVKYLQTQPLVKKLY
                     HPSLPENQGHEIAARQQKGFGAMLSFELDGDEQTLCRFLGGLSLFTLAESLGGVESLI
                     SHAATMTHAGMAPEARAAAGISETLLRISTGIEDGEDLIADLENGFRAANKG"
     gene            29619..29936
                     /gene="metJ"
                     /locus_tag="OO357_001017"
     CDS             29619..29936
                     /gene="metJ"
                     /locus_tag="OO357_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015855437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="met regulon transcriptional regulator MetJ"
                     /protein_id="HCR5886046.1"
                     /translation="MAEWSGEYISPYAEHGKKSEQVKKITVSIPLKVLKILTDERTRR
                     QVNNLRHATNSELLCEAFLHAFTGQPLPDDADLRKERSDEIPEAAKEIMREMGINPET
                     WEY"
     gene            30120..30728
                     /locus_tag="OO357_001018"
     CDS             30120..30728
                     /locus_tag="OO357_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiX family permuted papain-like enzyme"
                     /protein_id="HCR5886047.1"
                     /translation="MKNRLLILSLLVSVPAFAWQPQTGDIIFQISRSSQSKAIQLATH
                     SDYSHTGMLVMRNKKPYVFEAVGPVKYTPLKQWIAHGEKGNYVVRRVEGGLSVEQQQK
                     LTQTAKRYLGKPYDFSFSWSDDRQYCSEVVWKVYQNALGMRVGEQQKLKEFDLSNPLV
                     QAKLKERYGKNIPLEETVVSPQAVFDAPQLTTVAKEWPLFSW"
     gene            complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO357_001019"
     CDS             complement(30789..31001)
                     /gene="rpmE"
                     /locus_tag="OO357_001019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HCR5886048.1"
                     /translation="MKKDIHPKYEEITASCSCGNVIKIRSTVGHDLNLDVCSKCHPFF
                     TGKQRDVATGGRVDRFNKRFNIPGSK"
     gene            31204..33402
                     /gene="priA"
                     /locus_tag="OO357_001020"
     CDS             31204..33402
                     /gene="priA"
                     /locus_tag="OO357_001020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HCR5886049.1"
                     /translation="MPVAHVALPVPLPRTFDYLLPEGMTVKAGCRVRVPFGKQQERIG
                     IVVSVSDASELPLTELKAVVEVLDGEPVFTHSVWRLLLWAADYYHHPIGDVLFHALPI
                     LLRQGRPAANAPMWYWFATEQGQAVDLNSLKRSPKQQQALAALRQGKIWRDQVATLEF
                     NDAALQALRKKGLCDLASETPEFSDWRTNYAVSGERLRLNTEQATAVGAIHSAADTFS
                     AWLLAGVTGSGKTEVYLSVLENVLAQGKQALVMVPEIGLTPQTIARFRERFNAPVEVL
                     HSGLNDSERLSAWLKAKNGEAAIVIGTRSALFTPFKNLGVIVIDEEHDSSYKQQEGWR
                     YHARDLAVYRAHSEQIPIILGSATPALETLCNVQQKKYRLLRLTRRAGNARPAIQHVL
                     DLKGQKVQAGLAPALITRMRQHLQANNQVILFLNRRGFAPALLCHDCGWIAECPRCDH
                     YYTLHQAQQHLRCHHCDSQRPVPRQCPSCGSTHLVPVGLGTEQLEQTLAPLFPDVPIS
                     RIDRDTTSRKGALEQQLAEVHRGGARILIGTQMLAKGHHFPDVTLVALLDVDGALFSA
                     DFRSAERFAQLYTQVAGRAGRAGKQGEVVLQTHHPEHPLLQTLLYKGYDAFAEQALAE
                     RRMMQLPPWTSHVIVRAEDHNNQHAPLFLQQLRNLILSSPLADDKLWVLGPVPALAPK
                     RGGRWRWQILLQHPSRVRLQHIISGTLALINTIPDSRKVKWVLDVDPIEG"
     gene            33558..34583
                     /gene="cytR"
                     /locus_tag="OO357_001021"
     CDS             33558..34583
                     /gene="cytR"
                     /locus_tag="OO357_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CytR"
                     /protein_id="HCR5886050.1"
                     /translation="MKAKKQETAATMKDVALKAKVSTATVSRALMNPDKVSQATRNRV
                     EKAAREVGYLPQPMGRNVKRNESRTILVIVPDICDPFFSEIIRGIEVTAANHGYLVLI
                     GDCAHQNQQEKTFIDLIITKQIDGMLLLGSRLPFDASIEEQRNLPPMVMANEFAPELE
                     LPTVHIDNLTAAFDAVNYLYEQGHKRIGCIAGPEEMPLCHYRLQGYVQALRRCGIMVD
                     PQYIARGDFTFEAGSKAMQQLLDLPQPPTAVFCHSDVMALGALSQAKRQGLKVPEDLS
                     IIGFDNIDLTQFCDPPLTTIAQPRYEIGREAMLLLLDQMQGQHVGSGSRLMDCELIIR
                     GSTRALP"
     gene            34675..35634
                     /gene="ftsN"
                     /locus_tag="OO357_001022"
     CDS             34675..35634
                     /gene="ftsN"
                     /locus_tag="OO357_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsN"
                     /protein_id="HCR5886051.1"
                     /translation="MAQRDYVRRSQPAPSRRKKSTSRKKQRNLPAVSPAMVAIAAAVL
                     VTFIGGLYFITHHKKEESETLQSQKVTGNGLPPKPEERWRYIKELESRQPGVRAPTEP
                     SAGGEVKTPEQLTPEQRQLLEQMQADMRQQPTQLVEVPWNEQTPEQRQQTLQRQRQAQ
                     QLAEQQRLAQQSRTTEQSWQQQTRTSQAAPVQAQPRQSKPASTQQPYQDLLQTPAHTT
                     AQSKPQQAAPVARAADAPKPTAEKKDERRWMVQCGSFRGAEQAETVRAQLAFEGFDSK
                     ITTNNGWNRVVIGPVKGKENADSTLNRLKMAGHTNCIRLAAGG"
     gene            35727..36257
                     /gene="hslV"
                     /locus_tag="OO357_001023"
     CDS             35727..36257
                     /gene="hslV"
                     /locus_tag="OO357_001023"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HCR5886052.1"
                     /translation="MTTIVSVRRNGHVVIAGDGQATLGNTVMKGNVKKVRRLYNDKVI
                     AGFAGGTADAFTLFELFERKLEMHQGHLVKAAVELAKDWRTDRMLRKLEALLAVADET
                     ASLIITGNGDVVQPENDLIAIGSGGPYAQAAARALLENTELSAREIAEKALDIAGDIC
                     IYTNHFHTIEELSYKA"
     gene            36267..37598
                     /gene="hslU"
                     /locus_tag="OO357_001024"
     CDS             36267..37598
                     /gene="hslU"
                     /locus_tag="OO357_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019841241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase ATPase subunit"
                     /protein_id="HCR5886053.1"
                     /translation="MSEMTPREIVSELDKHIIGQDNAKRSVAIALRNRWRRMQLNEEL
                     RHEVTPKNILMIGPTGVGKTEIARRLAKLANAPFIKVEATKFTEVGYVGKEVDSIIRD
                     LTDAAVKMVRVQAIEKNRYRAEELAEERILDVLIPPAKNNWGQTEQQQEPSAARQAFR
                     KKLREGQLDDKEIEIDLAAAPMGVEIMAPPGMEEMTSQLQSMFQNLGGQKQKARKLKI
                     KDAMKLLIEEEAAKLVNPEELKQDAIDAVEQHGIVFIDEIDKICKRGESSGPDVSREG
                     VQRDLLPLVEGCTVSTKHGMVKTDHILFIASGAFQIAKPSDLIPELQGRLPIRVELQA
                     LTTSDFERILTEPNASITVQYKALMATEGVNIEFTDSGIKRIAEAAWQVNESTENIGA
                     RRLHTVLERLMEEISYDASDLSGQNITIDADYVSKHLDALVADEDLSRFIL"
     gene            37665..38591
                     /gene="menA"
                     /locus_tag="OO357_001025"
     CDS             37665..38591
                     /gene="menA"
                     /locus_tag="OO357_001025"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HCR5886054.1"
                     /translation="MTEQQISRTQAWLESLRPKTLPLAFAAIIVGTALAWWQGHFDPL
                     VALLALITAGLLQILSNLANDYGDAVKGSDKPDRIGPLRGMQKGVITQQKMKRALIIT
                     VVLICLSGLALVAVACHTLADFVGFLILGGLSIIAAITYTVGNRPYGYIGLGDISVLV
                     FFGWLSVMGSWYLQAHTLIPALILPATACGLLATAVLNINNLRDINSDRENGKNTLVV
                     RLGEVNARRYHACLLMGSLVCLALFNLFSLHSLWGWLFLLAAPLLVKQARYVMREMDP
                     VAMRPMLERTVKGALLTNLLFVLGIFLSQWAA"
     gene            38684..39169
                     /gene="rraA"
                     /locus_tag="OO357_001026"
     CDS             38684..39169
                     /gene="rraA"
                     /locus_tag="OO357_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001392924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E activity regulator RraA"
                     /protein_id="HCR5886055.1"
                     /translation="MKYDTSELCDIYQEDVNVVEPLFSNFGGRASFGGQIITVKCFED
                     NGLLYDLLEQNGRGRVLVVDGGGSVRRALVDAELARLAVQNEWEGLVIYGAVRQVDDL
                     EELDIGIQAMAAIPVGAAGEGIGESDVRVNFGGVTFFSGDHLYADNTGIILSEDPLDI
                     E"
     gene            complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO357_001027"
     CDS             complement(39254..39499)
                     /gene="zapB"
                     /locus_tag="OO357_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring assembly protein ZapB"
                     /protein_id="HCR5886056.1"
                     /translation="MTMSLEVFEKLEAKVQQAIDTITLLQMEIEELKEKNNSLSQEVQ
                     NAQHQREELERENNHLKEQQNGWQERLQALLGRMEEV"
     gene            39925..40770
                     /gene="glpF"
                     /locus_tag="OO357_001028"
                     /pseudo
     CDS             39925..40770
                     /gene="glpF"
                     /locus_tag="OO357_001028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312879.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol uptake facilitator protein GlpF"
     gene            40810..42300
                     /gene="glpK"
                     /locus_tag="OO357_001029"
     CDS             40810..42300
                     /gene="glpK"
                     /locus_tag="OO357_001029"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HCR5886057.1"
                     /translation="MVALDQGTTSSRAVVMDHDANIISVSQREFEQIYPKPGWVEHDP
                     MEIWATQSSTLVEVLAKADISSDQIAAIGITNQRETTIVWEKETGKPIYNAIVWQCRR
                     TAEICEHLKRDGLEDYIRSNTGLVIDPYFSGTKVKWILDHVEGSHERARRGELLFGTV
                     DTWLIWKMTQGRVHVTDYTNASRTMLFNIHTLDWDDKMLEVLDIPREMLPEVRRSSEV
                     YGQTNIGGKGGTRIPISGIAGDQQAALFGQLCVKEGMAKNTYGTGCFMLMNTGEKAVK
                     SENGLLTTIACGPTGEVNYALEGAVFMAGASIQWLRDEMKLINDAYDSEYFATKVQNT
                     NGVYVVPAFTGLGAPYWDPYARGAIFGLTRGVNANHIIRATLESIAYQTRDVLEAMQA
                     DSGIRLHALRVDGGAVANNFLMQFQSDILGTRVERPEVREVTALGAAYLAGLAVGFWQ
                     NLDELQEKAVIEREFRPGIETTERNYRYAGWKKAVKRAMAWEEHDE"
     gene            42530..43540
                     /gene="glpX"
                     /locus_tag="OO357_001030"
     CDS             42530..43540
                     /gene="glpX"
                     /locus_tag="OO357_001030"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5886058.1"
                     /translation="MRRELAIEFSRVTESAALAGYKWLGRGDKNTADGAAVNAMRIML
                     NQVNIDGTIVIGEGEIDEAPMLYIGEKVGTGRGDAVDIAVDPIEGTRMTAMGQANALA
                     VLAVGDKGCFLNAPDMYMEKLIVGPGAKGTIDLNLPLADNLRNVAAALGKPLSELTVT
                     ILAKPRHDAVIAEMQQLGVRVFAIPDGDVAASILTCMPDSEVDVLYGIGGAPEGVVSA
                     AVIRALDGDMNGRLLARHDVKGDNEENRRIGEQELARCKAMGIEAGKVLRLGDMARSD
                     NVIFSATGITKGDLLEGISRKGNIATTETLLIRGKSRTIRRIQSIHYLDRKDPEMQVH
                     IL"
     gene            43637..44383
                     /gene="fpr"
                     /locus_tag="OO357_001031"
     CDS             43637..44383
                     /gene="fpr"
                     /locus_tag="OO357_001031"
                     /EC_number="1.18.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin--NADP(+) reductase"
                     /protein_id="HCR5886059.1"
                     /translation="MADWVTGKVTKVQNWTDALFSLTVHAPVLPFTAGQFTKLGLEID
                     GERVQRAYSYVNSPDNPDLEFYLVTVPDGKLSPRLAALKPGDEVQVVSEAAGFFVLDE
                     VPDCETLWMLATGTAIGPYLSILQLGKDLDRFKNLVLVHAARYAADLSYLPLMQELEK
                     RYEGKLRIQTVVSRETAAGSLTGRIPALIESGELESAIGLPMNKETSHVMLCGNPQMV
                     RDTQQLLKETRQMTKHLRRRPGHMTAEHYW"
     gene            complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO357_001032"
     CDS             complement(44388..44816)
                     /gene="uspD"
                     /locus_tag="OO357_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspD"
                     /protein_id="HCR5886060.1"
                     /translation="MAYKHIGVAISGNEEDALLVNKALELARHNDAHLTLIHIDDGLS
                     ELYPGIYFPATEDILQLLKNKSDNKLYKLTKNIQWPKTKLRIERGEMPETLLEIMQKE
                     QCDLLVCGHHHSFINRLMPAYRGMINKMSADLLIVPFIDK"
     gene            complement(44843..45142)
                     /locus_tag="OO357_001033"
     CDS             complement(44843..45142)
                     /locus_tag="OO357_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF406 domain-containing protein"
                     /protein_id="HCR5886061.1"
                     /translation="MKDVVDKCSTKGCAIDIGTVIDNDNCTSKFSRFFATREEAESFM
                     TKLKELAAAASSADEGASVAYKIKDLEGQVELDAAFTFSCQAEMIIFELSLRSLA"
     gene            complement(45354..45794)
                     /locus_tag="OO357_001034"
                     /pseudo
     CDS             complement(45354..45794)
                     /locus_tag="OO357_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312873.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
     gene            45895..46494
                     /locus_tag="OO357_001035"
     CDS             45895..46494
                     /locus_tag="OO357_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiiQ family protein"
                     /protein_id="HCR5886062.1"
                     /translation="MKPGCTLFFLLCSALTVTTTAHAQTPDTATTAPYLLAGAPTFDL
                     SISQFREDFNSQNPSLPLNEFRAIDSSPDKANLTRAASKINENLYASTALERGTLKIK
                     SIQMTWLPIQGPEQKAAKAKAQEYMAAVIRTLTPLMTKTQSQKKLQSLLTAGKNKRYY
                     TETEGALRYVVADNGEKGLTFAVEPIKLALSESLEGLNK"
     gene            46602..47369
                     /gene="tpiA"
                     /locus_tag="OO357_001036"
     CDS             46602..47369
                     /gene="tpiA"
                     /locus_tag="OO357_001036"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HCR5886063.1"
                     /translation="MRHPLVMGNWKLNGSRHMVHELVSNLRKELAGVAGCAVAIAPPE
                     MYIDMAKREAEGSHIMLGAQNVDLNLSGAFTGETSAAMLKDIGAQYIIIGHSERRTYH
                     KESDELIAKKFAVLKEQGLTPVLCIGETEAENEAGKTEEVCARQIDAVLKTQGAAAFE
                     GAVIAYEPVWAIGTGKSATPAQAQAVHKFIRDHIAKVDANIAEQVIIQYGGSVNASNA
                     AELFAQPDIDGALVGGASLKADAFAVIVKAAEAAKQA"
     gene            complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO357_001037"
     CDS             complement(47424..48179)
                     /gene="cdh"
                     /locus_tag="OO357_001037"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HCR5886064.1"
                     /translation="MKKAGLLFLVMIVIAVVAAGIGYWKLTGEESDTLRKIVLEECLP
                     NQQQNQNPSPCAEVKPNAGYVVLKDLNGPLQYLLMPTYRINGTESPLLTDPSTPNFFW
                     LAWQARDFMSKKYGQPVPDRAVSLAINSRTGHTQNHFHIHISCIRPDVREQLDKNLAN
                     ISSRWLPLPGGLRGHEYLARRVTESELVQRSPFMMLAEEVPEAREHMGSYGLAMVRQS
                     DNSFVLLATQRNLLTLNRASAEEIQDHECEILR"
     gene            complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO357_001038"
     CDS             complement(48286..49275)
                     /gene="sbp"
                     /locus_tag="OO357_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/thiosulfate ABC transporter
                     substrate-binding protein Sbp"
                     /protein_id="HCR5886065.1"
                     /translation="MNKWGVGLTFLLAATSVMAKDIQLLNVSYDPTRELYEQYNKAFS
                     AHWKQQTGDNVVIRQSHGGSGKQATSVINGIEADVVTLALAYDVDAIAERGRIDKEWI
                     KRLPDNSAPYTSTIVFLVRKGNPKQIHDWNDLIKPGVSVITPNPKSSGGARWNYLAAW
                     GYALHHNNNDQAKAQDFVRALYKNVEVLDSGARGSTNTFVERGIGDVLIAWENEALLA
                     ANELGKDKFEIVTPSESILAEPTVSVVDKVVEKKGTKEVAEAYLKYLYSPEGQEIAAK
                     NYYRPRDAEVAKKYENAFPKLKLFTIDEEFGGWTKAQKEHFANGGTFDQISKR"
     gene            complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO357_001039"
     CDS             complement(49595..50557)
                     /gene="pfkA"
                     /locus_tag="OO357_001039"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014072359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HCR5886066.1"
                     /translation="MIKKIGVLTSGGDAPGMNAAIRGVVRSALTEGLEVMGIYDGYLG
                     LYEDRMVQLDRYSVSDMINRGGTFLGSARFPEFRDENIRAVAIENLKKRGIDALVVIG
                     GDGSYMGAMRLTEMGFPCIGLPGTIDNDIKGTDYTIGFFTALSTVVEAIDRLRDTSSS
                     HQRISVVEVMGRYCGDLTLAAAIAGGCEFVVVPEVEFSREDLVNEIKAGIAKGKKHAI
                     VAITEHMCDVDELAHFIEKETGRETRATVLGHIQRGGSPVPYDRILASRMGAYAIDLL
                     LAGYGGRCVGIQNEQLVHHDIIDAIENMKRPFKGDWLDCAKKLY"
     gene            complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO357_001040"
     CDS             complement(50738..51640)
                     /gene="fieF"
                     /locus_tag="OO357_001040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709720.2"
                     /note="FieF, a metal efflux transporter, is a member of
                     the CDF (cation diffusion facilitator) family of
                     transporters; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family cation-efflux transporter FieF"
                     /protein_id="HCR5886067.1"
                     /translation="MNQSYGRLVSRAAIAATAMASLLLLIKIFAWWYTGSVSILAALV
                     DSLVDIGASLTNLLVVRYSLQPADDNHSFGHGKAESLAALAQSMFISGSALFLFLTGI
                     QHLVSPTPMTDPGVGVIVTIVALICTIILVSFQRWVVRRTQSQAVRADMLHYQSDVMM
                     NGAILLALGLSWYGWHRADALFALGIGIYILYSALRMGYEAVQSLLDRALPDEERQEI
                     IDIVTSWPGVSGAHDLRTRQSGPTRFIQIHLEMEDSLPLVQAHMVADQVEQAILRRFP
                     GSDVIIHQDPCSVVPREGKRSMLS"
     gene            complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO357_001041"
     CDS             complement(51788..52288)
                     /gene="cpxP"
                     /locus_tag="OO357_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell-envelope stress modulator CpxP"
                     /protein_id="HCR5886068.1"
                     /translation="MRIVTAAVMASTLAVSSLSHAAEVGSGDNWHPGEELTQRSTQSH
                     MFDGISLTEHQRQQMRDLMQQARHEQPPVNVSELETMHRLVTAENFDENAVRAQAEKM
                     ANEQIARQVEMAKVRNQMYRLLTPEQQAVLNEKHQQRMEQLRDVTQWQKSSSLKLLSS
                     SNSRSQ"
     gene            52438..53136
                     /gene="cpxR"
                     /locus_tag="OO357_001042"
     CDS             52438..53136
                     /gene="cpxR"
                     /locus_tag="OO357_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001033718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response regulator transcription
                     factor CpxR"
                     /protein_id="HCR5886069.1"
                     /translation="MNKILLVDDDRELTSLLKELLEMEGFNVIVAHDGEQALDLLDDS
                     IDLLLLDVMMPKKNGIDTLKALRQTHQTPVIMLTARGSELDRVLGLELGADDYLPKPF
                     NDRELVARIRAILRRSHWSEQQQNNDNGSPTLEVDALVLNPGRQEASFDGQTLELTGT
                     EFTLLYLLAQHLGQVVSREHLSQEVLGKRLTPFDRAIDMHISNLRRKLPDRKDGHPWF
                     KTLRGRGYLMVSAS"
     gene            53133..54506
                     /gene="cpxA"
                     /locus_tag="OO357_001043"
     CDS             53133..54506
                     /gene="cpxA"
                     /locus_tag="OO357_001043"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress sensor histidine kinase CpxA"
                     /protein_id="HCR5886070.1"
                     /translation="MIGSLTARIFAIFWLTLVLVLMLVLMLPKLDSRQMTELLDSEQR
                     QGLMIEQHVEAELANDPPNDLMWWRRLFRAIDKWAPPGQRLLLVTTEGRVIGAERSEM
                     QIIRNFIGQADNADHPQKKKYGRVELVGPFSVRDGEDNYQLYLIRPASSSQSDFINLL
                     FDRPLLLLIVTMLVSTPLLLWLAWSLAKPARKLKNAADEVAQGNLRQHPELEAGPQEF
                     LAAGASFNQMVTALERMMTSQQRLLSDISHELRTPLTRLQLGTALLRRRSGESKELER
                     IETEAQRLDSMINDLLVMSRNQQKNALVSETIKANQLWSEVLDNAAFEAEQMGKSLTV
                     NFPPGPWPLYGNPNALESALENIVRNALRYSHTKIEVGFAVDKDGITITVDDDGPGVS
                     PEDREQIFRPFYRTDEARDRESGGTGLGLAIVETAIQQHRGWVKAEDSPLGGLRLVIW
                     LPLYKRS"
     gene            complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="OO357_001044"
     CDS             complement(54612..55286)
                     /gene="yiiM"
                     /locus_tag="OO357_001044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein"
                     /protein_id="HCR5886071.1"
                     /translation="MRYPVDVYTGKIQAYPEGKPSAIAKIQVDGELMLTELGLEGDEQ
                     AEKKVHGGPDRALCHYPREHYLYWVREFPEQAELFVAPAFGENLSTDGLTESNVHMGD
                     IFRWGEALIQVSQPRSPCYKLNYHFDISDIAQLMQNTGKVGWLYSVIAPGKVSADAPL
                     ELVSRVSDVTVQEAAAIAWHMPFDDDQYHRLLSAAGLSKSWTRTMQKRRLSGKIEDFS
                     RRLWGK"
BASE COUNT        13495 a        15017 c        14165 g        12743 t
ORIGIN      
        1 gaatgctgac aaatttctgc ctgatgcggc gtaaacgcct tatccgccta cgaagtgtcg
       61 cactttgtag gcgtggtaaa ctccccattt tcaggggagc aatataaaca aattactcta
      121 accgagcctg cgcaaaagca atcgcctgcg ccacctgctg cggtgagacg ccgccttttg
      181 ccgcacgctt ttcgaggcac gattgtagcg acagaatcgg atagacatct tcgccaatca
      241 cctgactgaa tttctgcaac tcactgagtg gcagatcttc cagcggtttg ccctgacgaa
      301 tggcttccac caccgcttca cccacaatat ggtgcgcctc gcggaaaggt acgcctttcg
      361 ccaccaggta atccgccagt tcagtggcgt tcgcataccc ctgctgcgcc gcttcctgac
      421 aacgcggacg tttcacctga atgccgtcca gtaccagcgc cgccatatgc aggcagtcca
      481 gccaggtatc gagcgcgtcg aacagacctt ctttgtcttc ctgcatatct ttgttgtaag
      541 ccagcggcaa acctttcagc gtcatcatca tgccggttaa cgcaccctgc acccggccgc
      601 atttaccgcg aatcagctcc agcgcatccg ggtttttctt ctgcggcatt aatgatgaac
      661 cggaagtcac gcggtccgaa agctccacaa accccgcttc gccggtgtta aagaaaatca
      721 gatcttcagc aaaacgcgac agatgcacca tgccgatagc ggcagcagaa agcagttcca
      781 acacatggtc acggtcagaa acgctgtcca gactgttacg ggtcgccgaa gcaaagccca
      841 gccagcctgc taactgttca cggtcgattt cataggccgt tcccgccagc gcgccacagc
      901 ctaatgggct gacatccaga cgcttaagcg catcctgcaa acggctttca tcacgcgcca
      961 gcatctcaac ataggccagg caccagtgcg cgaacgtcac cggctgggcg cgttgcaggt
     1021 gagtgtaacc tggcattacc gcgtcctgat tgttttgtgc ggtttccacc agcgccgatt
     1081 gcagctgccg gttagccgtc agtaactcgc taacggtatc tttgcaccac agtttcaggt
     1141 cagtcgctac ctgatcatta cggctacgcc cggtatgcag ctttttgcct aactggccca
     1201 ctttgtcgat cagtttgcct tccacccagc tatggatatc ttcggcgtcg ctttcaagga
     1261 tttgttgtgg cctggcgcga acatcttcca gcaacacgtt cagcgcctct tccagttgcg
     1321 cctgctcttc tgcggttaac acgccgaccg ttaccaaggc tttggaccag gccacagagc
     1381 caacaatatc ctgctccgcc agacggtaat caaagcgcag tgagtcgttg aattgtttga
     1441 accgttgatc tgctgcctgg gtaaaacgcc cgccccaaag tgccataact ctgtttcctt
     1501 attttgaaat tcaatgccgg aaagctgcgc ctccggctaa caaaacttaa gctaaaatcc
     1561 gcgtacccat cggcataccg ttaaacagtg ccggaagctg ctccgcataa cgccaagaag
     1621 cgatatctac cggacggccc agcgtgcggg ccgcatccag cgccgcgttc actttcacta
     1681 tcatgccgtc agtaataatg ccctgctcaa tcagttgttc tgctttcgcg gcggtcattt
     1741 cggcaatgcg ttgccctttg ccgtcgagaa tgccgctgac gtcggagagc aaaatcagat
     1801 ccgcgcccag cgttgccgcc agcgccgttg ccgcctggtc ggcattgacg ttcatcagtt
     1861 gcccttcgtc tgttacgcca atggagctga ccaccggcag ataaccgttc tccagcaagg
     1921 agttgataag cttaggcgaa cctggctgcg ccagtccaac atgacctaac tcttcatcaa
     1981 gctgggtcac tttgacgctg tcgccgtcac cgagaaacaa acctacggcc gcaatctgat
     2041 gtttcttcgc ccatgccaac agggttttat ttgccgttcc cgccagtgct ccggtgataa
     2101 tgtctatctg atcagcaggc gtcacccgca ggccgttttt ctttttcacc ggcagattca
     2161 gccctttcat cagctcatcc accacgcaac cgccgccgtg cacaatcacc agcggacgct
     2221 gatgtgactc acgataattc accaatgcgc taaacagacg ttccagcgcc tcttcactat
     2281 ccagcagtac gccgcccagt ttgataatta atggattcat cattgcaccc ttaaataaga
     2341 gactgcgttt ccgcatagcc gaaacgaata ttggcgcact gtaccgcttg tgccgccgcg
     2401 cctttcagta agttgtcttc ggtcgccaca ataatcagat gctcgccctg aacggcaaac
     2461 ccgatatcgc aaaatggcag cccaacgaca tttttcagcg ccggaacgcc tttgtcatac
     2521 agccgcacca gcggtttatg ggcatacgcc tgctgtaacg cttgcgcgac ttgcgcctgg
     2581 gtcacacccg atttcaggcg gcaggtaatg gtttcgagaa tgccgcgcgg gaaattgccc
     2641 agatgtgggg tgaagataac gtcagcaccg aggtgtgtgg cgatctctgg ttgatggcga
     2701 tgagtaaaga cgccatacgg ttgcaggcta acttcacaaa aactatttga aatcgctgcc
     2761 ttacgtcctg caccgctcac gccgctggtg gcgttgatca ccggccactg attgaggtca
     2821 agaagatcgg catcaatcaa cggtttcagc gccagctgtg ccgccgtcgg ataacagcct
     2881 ggcaccgcaa tcaaattcgc ttcttttaat ttattaccgc accactccgc cagaccgtag
     2941 gctgcctgtt ccagcagttc cgggtattga tgggtaaagc cgtaatattt ttcatagaag
     3001 gtggcgtcgt taacacgaaa cgcgccggaa aggtcgaaca ccacgcagcc cgcttcaaga
     3061 aactgcggcg ctaaatcgtg gctaacttca tgggcggtgg cgagaaacac tacgtccacc
     3121 cctgggctaa actcgctgat atccgacatc ggctgcaacg gcagctcaac gatgcctttt
     3181 agctgcggat gcaaatcgga gattaacttt cccgcatcat tgctttgcgc tgaaacagtc
     3241 aaagcggtta tgttcatatg cggatggcga tttacatagg tcactagctc tgcgccagcg
     3301 tagccgctgg cacccacaat cagcgtattc aacatcgggg ctattcacct tcttatgtct
     3361 ggttgccagg ttaaacgtaa aacattcacc ttacggctgg tgggttttat tacgctcaac
     3421 gttagtgtat ttttattcat aaatactgca tgaatattga tactatcatg accagaggtg
     3481 tgtcaacaat gaaaaacaaa ttaccgccat ttatcgagat ttaccgcgct ctgattgcca
     3541 caccttcaat aagcgctacg gaagaggcac tcgatcaaag caatgcagat ttaatcactc
     3601 tgctggcgga ctggtttaaa gatttgggct tcaatgtgga agtgcagcct gttccaggaa
     3661 ctcgcaacaa atttaatatg ctggccagtt acggacaggg ggctggcggc ttgttgctgg
     3721 cggggcatac cgatacggtg ccatttgatg acggtcgctg gacgcgcgat ccgtttacac
     3781 tgacggagca tgacggcaag ctttacggct taggcaccgc cgacatgaaa ggcttttttg
     3841 cgtttatcct tgatgcgcta cgcgatgtcg acgtcacgaa actgaaaaaa ccgctctaca
     3901 ttctggcgac tgctgatgaa gaaaccagta tggccggagc gcgttatttt gccgaaacta
     3961 ccgccctgcg cccggattgc gccatcattg gcgaaccgac gtcactacaa ccggtacgcg
     4021 cacataaagg tcatatctct aacgccatcc gtattcaggg ccagtcgggg cactccagcg
     4081 atccagcacg cggaattaac gctatcgaac taatgcacga cgccatcggg catattttgc
     4141 aattgcgcga taacctgaaa gaacgttatc actacgaagc atttaccgtg ccatacccta
     4201 cgctcaacct cgggcatatt cacggtggcg acgcttctaa ccgtatttgc gcttgctgtg
     4261 agttgcatat ggatattcgt ccgcagcctg gcatgacact caatgaactt aatggtttgc
     4321 tcaacgatgc attggctccg gtgagcgaac gctggccggg tcgtctgacg gtcgacgagc
     4381 tgcatccgcc gatccctggc tatgaatgcc caccgaatca tcaactggtt gaagtggttg
     4441 agaaattgct cggagcaaaa accgaagtgg tgaattactg taccgaagcg ccgtttattc
     4501 aaacgttatg cccgacgctg gtgctcggtc cgggttccat caatcaagct catcagcctg
     4561 atgaatatct ggagacgcgg tttattaaac ccacccgtga gttgattatc caggtaattc
     4621 accatttttg ctggcattaa aacgtaggcc ggataaggcg ctcgcgccgc atccggcacc
     4681 gttgccaaac tccggtgccg caataatgtc ggatgcgatg cttgcgcatc ttatccgacc
     4741 tacacctttg gtgttatgta tggcgatttt ttaacatttc cataagttac gcttatttaa
     4801 agcgtcgtga atttaatggc gtaaattcct gctatttatt cgtttgctga agcgatttcg
     4861 cagcatttga cgtcaccgct tttacgtggc tttataaaag acgacgaaaa gcaaagcccg
     4921 agcatattcg cgccaatgcg acgtgaagga tacagggcta tcaaacgata agatggggtg
     4981 tctggggtaa tatgaacgaa caatattccg cattgcgtag taatgtcagt atgctcggca
     5041 aagtgctggg agaaaccatc aaggatgcgt tgggagaaca cattcttgaa cgcgtagaaa
     5101 ctatccgtaa gttgtcgaaa tcttcacgcg ctggcaatga tgctaaccgc caggagttgc
     5161 tcaccacctt acaaaatttg tcgaacgacg agctgctgcc cgttgcgcgt gcgtttagtc
     5221 agttcctgaa cctggccaac accgccgagc aataccacag catttcgccg aaaggcgaag
     5281 ctgccagcaa cccggaagtg atcgcccgca ccctgcgtaa actgaaaaac cagccggaac
     5341 tgagcgaaga caccatcaaa aaagcagtgg aatcgctgtc gctggaactg gtcctcacgg
     5401 ctcacccaac cgaaattacc cgtcgtacac tgatccacaa aatggtggaa gtgaacgcct
     5461 gtttaaaaca gctcgataac aaagatatcg ctgactacga acgcaaccag ctgatgcgtc
     5521 gcctgcgcca gttgatcgcc cagtcatggc ataccgatga aatccgtaag ctgcgtccaa
     5581 gcccggtaga tgaagccaaa tggggctttg ccgtagtaga aaacagcctg tggcaaggcg
     5641 taccgaatta cctgcgcgaa ctgaacgaac aactggaaga gaatctcggc tacaaactgc
     5701 ccgtcgaatt tgttccggta cgttttacct cgtggatggg cggtgaccgc gacggcaacc
     5761 cgaacgtcac tgccgatatc acccgccacg tcctgctgct cagccgctgg aaagccaccg
     5821 atctgttcct gaaagatatt caggttctgg tttctgaact gtcgatggtt gaagcgaccc
     5881 ctgaactgct ggcgctggtt ggcgaagaag gtgccgcaga accgtatcgc tatctgatga
     5941 aaaacctgcg ttctcgcctg atggcgacac aggcatggct ggaagcgcgc ctgaaaggcg
     6001 aagaactgcc aaaaccagaa gggctgctga cacaaaacga agaactgtgg gaaccgctct
     6061 acgcttgcta ccagtcactt caggcgtgtg gcatgggtat tatcgccaac ggcgatctgc
     6121 tcgacaccct gcgccgcgtg aaatgtttcg gcgtaccgct ggtccgtatt gatatccgtc
     6181 aggagagcac ccgccatacc gaagcgctgg gcgagctgac ccgctacctc ggtatcggcg
     6241 actacgaaag ctggtcagag gccgacaaac aggcgttcct gatccgcgaa ctgaactcca
     6301 aacgtccgct tctgccgcgc aactggcaac caagcgccga aacgcgcgaa gtgctcgata
     6361 cctgccaggt gattgccgaa gcgccgcaag gctccattgc cgcctacgtg atctcgatgg
     6421 cgaaaacgcc gtccgacgta ctggctgtcc acctgctgct gaaagaagcg ggtatcgggt
     6481 ttgcgatgcc ggttgctccg ctgtttgaaa ccctcgatga cctgaacaac gccaacgatg
     6541 tcatgaccca gctgctcaat atcgactggt atcgcggcct gattcagggc aaacagatgg
     6601 tgatgattgg ctattccgac tcagcaaaag atgcgggcgt gatggcagct tcctgggcgc
     6661 aatatcaggc acaggatgca ttaatcaaaa cctgcgaaaa agcgggtatt gagctgacgt
     6721 tgttccacgg tcgcggcggt tccattggtc gcggcggcgc acctgcccat gcggcgctgc
     6781 tgtcacaacc gccaggaagc ctgaaaggtg gcctgcgcgt gactgaacag ggcgagatga
     6841 tccgctttaa atatggtctg ccagaaatca ccgtcagcag cctgtcgctt tacaccgggg
     6901 cgattctgga agccaacctg ctgccaccac ctgagccgaa agagagctgg cgtcgcatta
     6961 tggatgaact gtcagtcatc tcctgcgatc tctaccgtgg ctacgtacgt gaaaacaaag
     7021 attttgtgcc ttacttccgc tccgctacgc cggaacaaga attgggcaaa ctgccgttgg
     7081 gttcacgtcc ggcgaaacgt cgcccaaccg gcggcgtcga gtcgctgcgc gccataccgt
     7141 ggatttttgc ctggacgcaa aaccgcctga tgctccccgc ctggctgggt gcaggtacgg
     7201 cgctgcaaaa agtggtcgaa gatggcaaac agagcgagct ggaagccatg tgccgcgatt
     7261 ggccattctt ctcgacgcgt ctcggcatgc tggagatggt cttcgccaaa gcagacctgt
     7321 ggttggcgga atactatgat caacgtctgg tagacaaagc actgtggccg ttaggtaaag
     7381 agttacgcaa cctgcaagaa gaagacatca aagtggtgct ggcgattgcc aacgattccc
     7441 atctgatggc cgatctgccg tggattgcag agtctattca gctacggaat atttacaccg
     7501 acccgctgaa cgtattgcag gccgagttgc tgcaccgctc ccgccaggca gaaaaagaag
     7561 gccaggaacc ggatcctcgc gtcgaacaag cgttaatggt cactattgcc gggattgcgg
     7621 caggtatgcg taataccggc taatcttcct cttctgcaaa ccctcgtgct tttgcgcgag
     7681 ggttttctga aatacttctg ttctaacacc ctcgttttca atatatttct gtctgcattt
     7741 tattcaaatt ctgaatatac cttcagatat ccttaaggaa ttgtcgttat attcggcgat
     7801 atttttttca agacaggttc ttactatgca ttccacagaa gtccaggcta aacccctttc
     7861 tagctggaaa gccctgggtt gggcactgct ctacttttgg tttttctcta ctctgctaca
     7921 ggccattatt tacatcagtg gttatagtgg caccaacggc attcgcgact cgctgttatt
     7981 cagctcgctg tggttgatcc cggtattcct ctttccgaag cggattaaaa ttattgccgc
     8041 agtgatcggc gtggtgctat ggacggcctc tctggcggcg ctgtgctact acgtcatcta
     8101 cgggcaggag ttctcgcaga gcgttctgtt tgtgatgttc gaaaccaaca ccaacgaagc
     8161 cagcgagtat ttaagccagt atttcagcct gaaaattgtg cttatcgcgc tggcctatac
     8221 ggcggtggca gttctgctgt ggacacgcct gcgcccggtc tatattccaa agccgtggcg
     8281 ttatgttgtc tcttttgccc tgctttatgg cttgattctg catccgatcg ccatgaatac
     8341 gtttatcaaa aacaagccgt ttgagaaaac gttggataac ctagcctcgc gtatggagcc
     8401 tgccgcaccg tggcaattcc tgaccggcta ttatcagtat cgtcagcaac taaactcgct
     8461 aacaaagtta ctgaatgaaa ataatgcctt gccgccactg gctaatttca aagatgaatc
     8521 gggtaacgaa ccgcgcactt tagtgctggt gattggcgag tcgacccagc gcggacgcat
     8581 gagtctgtac ggttatccgc gtgaaaccac gccggagctg gatgcgctgc ataaaaccga
     8641 tccgaatctg accgtgttta ataacgtagt tacgtctcgt ccgtacacca ttgaaatcct
     8701 gcaacaggcg ctgacctttg ccaatgaaaa gaacccggat ctgtatctga cgcagccgtc
     8761 gctgatgaac atgatgaaac aggcgggtta taaaaccttc tggatcacca accagcagac
     8821 gatgaccgcc cgcaatacca tgctgacggt attttcgcgc cagaccgaca agcagtacta
     8881 catgaaccag caacgtacgc agagtgcgcg tgaatacgac accaacgtgc tgaagccgtt
     8941 ccaggatgtg ctgaatgacc ctgcgccgaa gaaactgatc atcgttcatc tgctgggtac
     9001 gcatatcaaa tacaaatacc gctacccgga aaatcagggc aagtttgatg gcaataccga
     9061 tcatgttccg ccaggattaa gcgcagaaga gctggaatca tataacgatt atgacaacgc
     9121 taacctgtat aacgatcatg tggttgccag cctgattaaa gactttaaag cggcagaccc
     9181 gaacggattc ctggtttact tctctgacca cggtgaagag gtttacgaca cgccgccgca
     9241 caaaactcag gggcgtaacg aagacaaccc gacgcgccac atgtacacca ttccgttcct
     9301 gctgtggacg tcggaaaaat ggcaagcgac tcatccccgt gatttctcac aggatgtcga
     9361 tcgtaaatac agcctggcgg aactgatcca cacctggtca gatttggcgg gcttatctta
     9421 cgacggttac gatccaaccc gttcagtggt gaatccgcag ttcaaagaaa ctacccgctg
     9481 gattggtaac ccgtacaaga aaaacgcgct gatcgattac gacactctgc cgtatggcga
     9541 tcaggtgggt aatcagtaga tgacctttgt atcgcgtctg gcgctatttg ccggatgcga
     9601 tactgccgca atgtttacat cgaactcccc tcaggccaca cttttcaggt ggcctttttt
     9661 aataatgcca gttactgttt tcccgtctac atacctaagg gaataacatg tatcacgacg
     9721 tcagctatct gctctcccgc ctgatcaacg gcccgctgtc cctgcgccag atttactttg
     9781 ccagcagtaa cggcccggtg cccgatcttg cgtatcaggt cgattttcca cggctggaaa
     9841 ttgtgctgga aggtgagttt gttgataccg gcgctggagc aacgttagtt cccggcgatg
     9901 tgctgtacgt tgccgctggt ggctggaatt tcccacaatg gaaaacaccc gctaccacct
     9961 ttagcgtgct gtttggcaaa cagcaactcg gcttcagcgt cgtgcaatgg gatggcaaac
    10021 aatatcaaaa tctggcgaag caacacgtcg cccgacgcgg cccacgcata ggttcttttc
    10081 tgctacaaac gctcaatgaa atgcaaatgc agccgcagga gcagcaaacg gcaaggctta
    10141 tcgtagccag cctgcttagc cactgccgcg atttgctcgg cagccaaatc cagaccgcct
    10201 cacgcagcca ggcactattt gaagctattc gcgattatat cgacgaacgc tatgcctccg
    10261 cgcttacccg cgaatctgtc gcacaggcat tctatatttc accaaactat ctctcgcacc
    10321 tgtttcaaaa aacgggggcc attggtttta acgaatacct gaatcacacg cgactggagc
    10381 acgctaagac gttattgaaa ggctatgacc tgaaggtaaa agaagtggcg cacgcctgcg
    10441 gctttgtcga cagcaactac ttctgccgtc tgtttcgtaa aaacaccgaa cactcgccgt
    10501 cggaatatcg ccggcagtac cacagccaac tgacagaaaa accgactact ccagaataag
    10561 atgggtttgc tgcggcgcag aaagcacttt gcgcaccgcg ctcattaccc gctgcggctc
    10621 acgcaggaat gcgtagatgc tgcattgcac atagcggcaa tgttcaaatc gctcggcacc
    10681 ggcaagctcg atatccgtaa tcaacagagc aacatccgcc cgacggatat cctcgtccgc
    10741 taaacgattc tccgttccca gcgcgccctg agtttcaatg ctgacgcccc acttctctaa
    10801 ctggcacaac ttttccagcc gttccgccgc catataagta tgcgccacgc cgcttacgca
    10861 ggcggttact gccaccaggt atgccatttt aacctcccac ggtaacctga aatccggccc
    10921 gttctgccat ttcgcgcatc gttgtcacat cggctgacga cggcgcaggc acctctttca
    10981 tcgaccatgt tttccccagc aggcggtatt tcggttcgcc gtactgatga aacggtaaca
    11041 gatggatctg cctgattttc agcggggtca gtacatccag cgcctgctgc atattctccc
    11101 ggctgagcgt gaaaccaggg atcaacggta aacgcgggat cacgttgaca ccctcactca
    11161 ccagcaaacg cagattctcc agcacgcgtg gcaggttcat cttcaccaca tcccgcgcct
    11221 gagtcgcgtc cataattttt aaatcgaaca gcacttcatc gcacaatttc gccagcggta
    11281 acagcttgct tgctggcgcg tctccggcag tttcaatggc gcatgagaca ccccacagcc
    11341 gcagtcgctg taaaaaacgg gtagcaaact ccgcctgcat taacacttcg ccgccagaaa
    11401 gcgtgacgcc gccgccggac gtgcgaaaga aaatgtcatc tttcatcact tcccgttcca
    11461 gagcgtcaag gctgatatcg cgaccaatcc gttcaaacgc cccggagggg cattcatccg
    11521 catcacgcaa acatttcgca cagtgcagac atttcgcctc tctgcgtacc gtctggattt
    11581 tgccggagat cgactccgga ttagcacacc acgggcaaag atgcggacag cctttaaaaa
    11641 agaccaccgt acgaatgccc tcgccgtcat tcagtgaata acgctgaatg ttgaaaatgc
    11701 gcgccacatc atcgcggcgc gtttccacaa cgttacagct gatgcgctgt ccggcggatg
    11761 atgtcatcct ggatctcctt cgacagttcg acaaagaagg cgctgtatcc ggcaacgcgc
    11821 accaccagcc cggcataatc ttgtgggcgc tgttgcgctt cccgcaacgt gtcggcgttc
    11881 accacgttaa actgaatatg ctgcaactta agctgggtaa acacccgtaa gaagtcggcc
    11941 agtttgcgta atcccgcttc accttccagg gtcgccggag tgaatttcac gttcagcaac
    12001 gtaccgttag acagcagcgt attatcgagc ttactgactg acttcagtac cgccgttggc
    12061 ccttgtgcgt cctgacccag cataggtgac aagccgccgt ctgccagttg ttccccggca
    12121 aaacgaccgt ctggcgtcgc gccaaccacc gatcccaacg gaacgtgagc agaaacggta
    12181 tacgatcccg gcgtgaagta accgccgcgc gggttctggt atttttccac ttctttgcag
    12241 tagtggcgca acagttcggc gctaatgtta tccacctcgt cgatatcgtt accgtatttc
    12301 tcaaagcggt taattaagcg agcgcggact ttttcgcctt ctggcgttgc aaagttggct
    12361 tttaataccg acagcaattc gtcaaaactt aaacgctgtt gctcaaaaac catccctttc
    12421 aacgcatgga gagaatcgct caggttggcg ataccaattc cctgtacgcc ggagaagtta
    12481 taacgcgcgc cgccatcggt aatatcgcga cctttttcca gacaatcgct gataaaagac
    12541 gagagcagcg gtacaggtgc ccagtcgcga tggccgatat cacaaatatt gctgccctca
    12601 accatcaggg tgatgtagtg gctgatcttg gcgcggatct gctccagtaa accttcataa
    12661 gtcagcgcag cattgccttc attttcatgc aggcagattt ccatcacttt cagcagatta
    12721 aacatcgcga tgtcatgcaa gccgtaggtt ctgccgggaa tagataattc cacacagccc
    12781 actacggaat agtcgcgcgc atcttccagc gaaacgccac ggttgaggaa cgctggcacc
    12841 accacttcat cgttaaagat ttgcggaata ccggtgccga ggcgaatggt ttcggcggtt
    12901 ttcatcagga acggcgtgtc gatcaaggcg ttagtgcgca cgccgaggtt cggttgcggt
    12961 aattgcacgc tttgataggc gtcaaggcaa aggaacgaaa gcacgttcac cgcgctacgt
    13021 ccgttctcgg ttaacccgcc gagcagtgcg gtatagccgg tcgggaaacc ggcgaaataa
    13081 cgcgcgctac tggtggagcg caacagcacg atgtcgttgc atttcaccca taaagattcg
    13141 agcagttctt tcaggaacgc cggatcttcg ccctgggtta aagatgcctg atagaacggc
    13201 aacatatact ggtcgaagcg ccctaaagac agcgaactgg cgttggattc gtattgcaga
    13261 atgatgttca tgtaccagaa taactggcac gcctgccaga acgtctgcgg cttatgttgc
    13321 gcgttatggc gggagatctc cgcaatagtc agcagctctt cgcgacgctg ggcatctgtg
    13381 cagtttgccg ccatcgtttc cgccagttcg gcgtaacgca aaatgtgttt ctgcgaggct
    13441 tccagcagta acaacgcggc ctgataaaag tgattctccg gctgttgctg acaatgttgc
    13501 tgcatctgtg ccaccagctc acccagaccg tgattcaaca ggcgtgggta atcaataata
    13561 atgtgcccct ggcctttatc cgtctggttg atgctgaaaa tctgcgtgtt ggtcgcggct
    13621 tttacttcat ctgtcatctg cccgttgatg aaatctttca tcgaacgttt ttcccagtac
    13681 gggaacaact cttcacggta gatacgttta tcttcttcgc tgatagcaaa gcggtcctgc
    13741 ggacgcgtcg ggaattgatc cagctctttc agcagccagt aagggtccat ttccggcgac
    13801 ataatcccgg cgcgcggttt tacggtgcgg ttaccggcaa tcagttcttc atcacgaatc
    13861 gaaatttcaa catgttcaag gatatacgct gttgctttcg cccggcgcaa tatcaccggt
    13921 tcgccttcgg tttgccgatg gctggcggta taaagcagcg cccgctccag cgagatttca
    13981 cgggtattgg caaacagtgc agttttgagg cgagagatac gattcgtcat gaggaactcc
    14041 tgtgggaaag cgaacccgcc cttgcgagcg ggagaagaac ttaagcggtt tgcgccaggt
    14101 gagcttcaat tttgctcata atggcgtcag cacgttttac tgcatcgctg atgttgacgc
    14161 gcacgatggt cttgccagca aaacgctctt caaacttgat gccgatatct ttggtcagaa
    14221 tcaccatatc agcgctcgcc acgtcttccg cagtcagttc attttccaga ccaattgacc
    14281 cctgggtttc tactttcact tcccagcctt tcgctttcgc ggcactttcc agcgcctctg
    14341 ctgccatata ggtatgggca acacctgaag gacatgcggt tactgcaata attttcgtca
    14401 tggctcgttc ctcaattaat taatttcaaa atccaaatcc aggtcgtctt ctttttcatc
    14461 agtcgaagac ccatttttac gcgccagact tttcagcacg ttcacacaaa cagccgtcac
    14521 cactgctccg acagccactg ctgcgatata acccaactta ccttcaacca ccggcagcac
    14581 aatcagacca ccccagcctg catagcactg cgcaccgacc agcgccgcag ttactgcacc
    14641 acaaactgaa ccgaccataa tggaaggaat gacacgcagc ggatcggcag cggcgaaagg
    14701 aatcgcccct tcagtaacgc caacacagcc catcaccagt gccgctttgc cggtttcgcg
    14761 ctcttctgcg gagaaatttt tacgaccaat cagcgtcgcc agccccatcc ccagcggcgg
    14821 gatacaaata cccacggcag caatggccac cacggtataa acaccctgag caacgcaaat
    14881 cagcatgaag gcataggcca ctttgttaac cggaccgccc atatcgaacg ccagcatcag
    14941 ccccatgatc accgccagca taacaatgct gccctgctgc atcccctgaa gccactgagt
    15001 caggctgttg gtcaacgccc ctacaggctc gcccaagccc cacatcatga tgcctgcggt
    15061 aatcagtgtg ccgatgatag gaataatgaa gatgggcatc accgagcgca gaactttatg
    15121 caccggaatt ttcttcaggt aatgcaccac gatgccgccg ataatcccgg cgatcagcgc
    15181 accaaagaag cccgcaccaa agctgttacc aacccaggca ccgatagcgc acggagccag
    15241 cgcagaacgc tctgcaatgg agtaaccaat gtaagcggcg aggaaaggca ccatcagcgt
    15301 caaacctgca acgccgatat caaacagttt tttcaaattc ggatcggcta cggcatccgg
    15361 cactgcgcct ttgccataca acatgacgga aaccgccagc aaaataccgc ccgataccac
    15421 gaagggaatc atgtgtgaaa cgcccgtcat taaatgctga cgggtatttt tcaggatctg
    15481 caccaactca ttcataagct gctcccgggc ttgcaaaagc ccatgtccat atttttgatg
    15541 gaggaaacaa taggcaatcg caggcggagc aaacagtgga taaaaaggcc atttactgga
    15601 cttttgtaat atcagtacaa aaatgcgatc cgcctcataa cttgcgataa tccggaagca
    15661 tgccgcaaaa cggcgtcttg tgagacgcgt cgcaaattga cggttacatt acatttgtcc
    15721 agcttttggc agatttgtca catggcaggc cgttaccgtg cgctcttatc ctgtaaacag
    15781 aaaaccgtac cgggagaaag gcaatggccc tgattgtgga atttatttgt gagctaccta
    15841 acggcgtaca tgcgcgtccg gcaagccacg ttgaaacgct gtgtaatact ttttcatctc
    15901 aaattgagtg gcataacctg cgtactgacc gtaaaggcaa cgccaaaagc gctctggcgc
    15961 tgattggcac cgatacgctg gtgggtgata actgccagct actgatttcc ggggccgacg
    16021 aacaggaagg gcaccagcgt ttaagccaat ggctgcgcga tgaattcccc cactgcgatg
    16081 cgccgctggc ggaagttaaa tctgacgaac tggaaccact gccggtttca ctgaccaatc
    16141 tgaatccgca aattatccgc gcccgcaccg tgtgcagcgg tagtgcaggc ggcattctga
    16201 cgccgatctc ttctttagat ctcaatgcgc tgagtaatct tcccgcagcc aaagacgttg
    16261 acgccgagca atccgcactg gaaaacggcc tgacgctggt actgaaaaac attgagtttc
    16321 gtctgctgga tagcgacggt gctaccagcg cgattctgga agcgcaccga tccctggctg
    16381 gcgatacttc cctgcacgaa catttactgg caggcgtcag cgccggatta agctgcgccg
    16441 aagcgattgt cgccagtgcg aatcactttt gcgaagagtt tgcccgttcc agcagcagat
    16501 acctgcaaga acgtgccctg gacgtacgcg acgtctgctt ccagttactc cagcaaatct
    16561 acggtgagca acgcttcccg gcaccgggca aactgacgca gtccgccatt tgtatggctg
    16621 atgaactgac ccccagccag ttcctcgaac tggataaaaa tcacctcaaa ggattgttac
    16681 tgaaaagcgg cggcaccacc tcacatacgg tgatccttgc ccgttcgttc aacattccga
    16741 cgctggttgg tgtggatatt gatgccctca ctccgtggca gcatcaaacg atttatatcg
    16801 acggcaacgc cggggcgatt gtggttgagc caggagaagc cgtagctcgt tattatcagc
    16861 aagaagcccg cgtacaggac gccctgcgtg agcaacagcg tgtctagctg acccaacaag
    16921 cccgtaccgc tgacggtatc cgcattgaaa ttgccgctaa catcgctcac tccgtggaag
    16981 cgcaggccgc attcggcaat ggtgcggaag gcgttggttt gttccgcact gaaatgctct
    17041 atatggatcg caccagtgca ccgggcgaaa gcgagctgta caacattttt tgtcaggcgc
    17101 tggagtctgc caacggacgc agcattattg tgcgcactat ggacattggc ggcgacaaac
    17161 ccgttgatta tctaaacatt cccgcagagg caaacccgtt cctcggttat cgcgccgtgc
    17221 gtatttatga agaatacgcg tcgttattta ccacacaact acgctcgatc ctgcgcgcct
    17281 ccgctcacgg cagcctgaaa atcatgatcc cgatgatctc ctcaatggaa gagatcttat
    17341 gggtgaaaga aaaactggca gaagccaaac agcaactacg taacgaacac attccgtttg
    17401 atgagaagat ccagctcggc attatgctgg aagtgccgtc ggtgatgttc atcatcgatc
    17461 aatgctgcga agagattgat ttctttagta ttggtagtaa tgacctgacg caatatctgc
    17521 tggcagtgga tcgcgataac gctaaggtta ctcgtcacta caacagcctg aatccggcat
    17581 tcttgcgggc gctcgattac gacgtgcagg cggtgcatcg ccagggtaaa tggattggtc
    17641 tgtgcggtga gctgggagcg aaaggttccg tgctgccgtt gctggtcggc ttagggctgg
    17701 atgaactcag catgagcgca ccatcaattc cggcggcgaa agcgcggatg gcgcaacttg
    17761 atagccgtga gtgccgcaag ttgctcaacc aggcaatggc ctgccgtact tcgctggaag
    17821 tggaacacct gctggcgcaa ttccgcatga cccaacagga cgcaccgctg gtcaccgccg
    17881 agtgcatcac gctggaaagt gactggcgca gcaaagaaga agtgctcaaa ggcatgaccg
    17941 ataacctgct gctggcgggc cgctgccgct atccgcgtaa actggaagcc gacttatggg
    18001 cgcgcgaggc cgttttctct accggtctgg gctttagttt tgccattcca cacagcaaat
    18061 cagaacacat tgagcaatcc accatcagcg tggcgcgtct gcaagcgccg gtgcgctggg
    18121 gcgatgatga agcgcaattc atcattatgt taaccctgaa caaacacgct gcgggcgatc
    18181 agcacatgcg cattttctcg cgcctcgctc gccgcatcat gcacgaagaa ttccgtaacg
    18241 cgctggttaa cgccgcctct gccgacgcta tcgccagcct gctgcaacat gaactggaac
    18301 tgtaaaagga aacatcatgg aactgtatct ggacaccgct aacgtcgcag aagtcgaacg
    18361 tctggcacgc atattcccga ttgccggggt gacaactaac ccgagcatta tcgctgccag
    18421 caaggagtcc atctgggaag tgctgccgcg tctgcaaaaa gcgattggtg atgagggcat
    18481 tctgtttgct cagaccatga gccgcgacgc gcaggggatg gtggaagaag cgaagcgcct
    18541 gcgcgacgct attccgggta ttgtggtgaa aatcccggtg acttccgaag gtctggcagc
    18601 aattaaaata ctgaaaaaat agggtattac tacacttggc accgctgtat atagcgccgc
    18661 agaagggtta ttagccgcac tggcaggggc aaaatacgtt gctccgtatg ttaaccgcgt
    18721 agatgcccag ggcggggacg gcattcgtac ggttcaggag ctgcaaacgc tgttagaaat
    18781 gcacgcgcca gaaagcatgg tgctggcagc cagctttaaa acgccgcgtc aggcgctgga
    18841 ctgtttactg gcaggatgtg aatccatcac cctgccctta gatgtagcgc aacaaatgct
    18901 caacacccct gcggtagagt cagctataga gaagttcgaa cacgactgga atgccgcatt
    18961 tggcactact catctctaaa ggagcaatta tggaccgcat tattcaatca ccgggtaaat
    19021 acatccaggg cgctgatgtg attaatcgtc tgggcgaata cctgaagccg ctggcagaac
    19081 gctggttagt ggtgggtgac aaatttgttt tagtttttgc tcaatccact gtcgagaaaa
    19141 gctttaaaga tgctggactg gtagtagaaa ttacgccgtt tggcggtgaa tgttcgcaaa
    19201 atgagatcga ccgtctgcgt ggcatcgcgg agactgcgca gtgtggcgca attctcggta
    19261 tcggtggcgg aaaaaccctc gatactgcca aagcactggc acatttcatg ggtgttccgg
    19321 tagcgatcgc accgactatc gcctcgaccg atgcaccgtg cagcgcattg tctgttatct
    19381 acaccgatga gggtgagttt gaccgctatc tgctgttgcc aaataacccg aatatggtca
    19441 ttgtcgacac caaaatcgtc gcgggcgcac ctgcacgtct gttagcagcg ggtatcggcg
    19501 atgcgctggc aacctggttt gaagcgcgtg cttgctctcg tagcggcgcg accaccatgg
    19561 cgggcggcaa gtgcacccag gcggcgctgg cactggctga actgtgctac aacaccctgc
    19621 tggaagaagg cgaaaaagcg atgcttgccg ccgaacagca tgtagtaacc ccggcgctgg
    19681 agcgcgtgat tgaagcgaac acctatttga gcggcgttgg ttttgaaagc ggcggctggc
    19741 ggcggctcac gcggtgcata acggcctgac cgctatcccg gacgcgcatc actattatca
    19801 cggtgaaaag tggcatttgg tacgctgacg cagctggtac tggaaaacgc gccggtggag
    19861 gaaatcgaaa ccgtagctgc acttagccat gcggtaggtt tgccaataac tctcgctcaa
    19921 ctggatatta aagaagatgt cccggcgaaa atgcgcattg ttgcaaaagc atcttgtgca
    19981 gaaggtgaaa ccatccacaa catgcctggc ggcgcgacgc cagatcaggt ttacgccgca
    20041 ctgctggtag ctgaccagta cggtcagcgt ttcctgcaag agtgggaata acctactcca
    20101 aactcccggc ttgtccggga gtttgaacgc aaaattgcct gatgcgctac gcttatcagg
    20161 cctacgcaat ctctgcaata tattgaattt gcgtgctttt gtaggccgga taaggcgttc
    20221 actccgcatc cggcactctg tgccagtaat ctgaaactcc cacgttgttc aggagttttt
    20281 tatccgcttc tggacatttt ctccacagaa caatcgttag cccggaaggt cgaatctgtg
    20341 actcttattt tcacggacga accagatgaa agcttcactg gcgctgctca gcctgctaac
    20401 ggcatttacc agccactcct taaaatctcc cgccgttcca ccaaccgtgg tacaaatcca
    20461 ggccaatacc aaccttgcca tcgcagacgg tgccagacag caaattggca gcacgctatt
    20521 ttacgatcgc agcttaccta tcctggcggt gatgttccgc aagaacgcgg tgtatgttcc
    20581 gatatggtga tccgcgcatt gcgcagccag aaggtcgatt tgcagaaact ggttcacgaa
    20641 gatatggcga agaattttgc tgagtacccg caaaaatgga agttaaagcg cccggacagc
    20701 aacatcgatc accgtcgggt gcctaatctg gaaacctggt ttagccgcca caacaaaaca
    20761 cgccccatca gtaaaaatgc cggtgactat caagcgggcg atattgtctc ctggcgatta
    20821 gataacgggc tggcgcatat tggggtggcg tcagatggct tcgcccgcga cggtacgccg
    20881 ctggtgatac ataacattgg cgcaggcgcg caggaagaag atgtgctgtt tagctggcgg
    20941 atggtggggc attatcgtta ttttgttaaa taagagatcg ccccacatga agaaaaaatt
    21001 cagatgcttt ttacactgac agcttctttt ttcttacggc tcaccagcgc cagtgccagc
    21061 acaatcagca caataccgct accttcaacg acccccggat tctcaccgag cagccaccag
    21121 gaaaagagta cgccacaaac gggaacggcc agtgtgctta agctggcaat actggcaggc
    21181 aagtttttca gcacaaacag ccataagctc cacgctagcg ccgtcgccag aatcgcactg
    21241 taggccagcg cccagaacac ggtgggctgc cagtcaattt cacgttgcgg caccagtaaa
    21301 gcgaccacac tcatcaccag cgccgcatac agcatctgcc aggatgttaa cgataataaa
    21361 tccacgcgcg gatgacgggc gtataggcgt ttagcaacaa tcgcgctcgc cccccaacta
    21421 acaccggaga ggattgccag catggcactt ttcatcgaag agaaatcgag ttgccacggc
    21481 tgcaacacca aaaataagcc gaaagcggca atcagaatcg cgaaatattg cccacgtcgc
    21541 aggcgttcac cgagaaacaa cgcggcgaaa atcaccaccc agaacggcat ggtatagctc
    21601 aggatcgcca ccttccccgc acctccgctg accaacgccc actgcgccag accaaccatc
    21661 ccgcaggttt gtaacagggc aatggctaag gtgtatttaa acggcgtcgg gcgcattccg
    21721 cgaccacgta ataaaaggac gatgaataaa acgagagcgc cgaaaatgca gcgtaaggcg
    21781 gtaaagtcga aggcaccgat gtaactggtg acttgcttca tgaaaatcca gctataactc
    21841 cagataagtg tgagcacaac caagccactg attgccagtg ggttgctctt tcctgcggca
    21901 gacatgagag atccagtgtg tagttttcct gtggacacta tacgccggta aagaaacttc
    21961 agcgactgcc tgaaagacag tcgctgaaga aggtcagatt acagcaggtc gaaacggtcg
    22021 aggttcatca ctttcaccca tgccgctacg aagtctttaa caaacttctc gtgggcatcg
    22081 ctgctggcgt aaacttccgc tactgcacgc aggacagagt tagaaccaaa taccagatcc
    22141 gcacggctgg ctgtgtattt cacttcgccg gtttcgcggt cacgtccttc gaacagctct
    22201 ttcgattcgt cagtcgcttt ccactcgtaa cgcatatcca gcaagttcac gaagaagtca
    22261 ttgctcaata cgccaacgcg gtcagtgaag acgccgtttt tgctgccatc gaagttggca
    22321 cccagtacac gcatcccgcc caccagcgca gtcatttccg gcgcggtcag cgtcagttgc
    22381 tgtgctttgt cgatcagcag tgactcggtg gtggaaacgt ccagacgagc gcgatagtta
    22441 cggaaaccgt cagcaattgg ctccagcagc tcaaacatct caatgtcagt ctgatcctga
    22501 cgcgcatcaa cgcgacccgg cgcaaacggt acatgaatgc tcaaacctgc ggcgcttgcg
    22561 gctttctcaa caccaaccac accagccagc acgatgatat ccgccagcga ggctttacca
    22621 gactctttct ggattttctc cagaacaggc agagcacgaa cggctgcggc gttcacatcc
    22681 cagtcgcgct gcggcattaa tgccagacgc gcaccgttgg caccaccgcg tttgtcgcca
    22741 ccacggaagg tagaagcaga tgcccaggcc accgatacca gctcactaac agacagacca
    22801 gaatccgcaa tcgcgaattt cagatcgata atgtcctgct cggtcgggtt gtagatcggc
    22861 tgcggcagcg gatcttgcca gatcagatct tctttcggca cttccggccc gatgtagcga
    22921 gatttcggcc ccatatccct gtgcgtcagt ttgaaccagg cacgggcaaa ggcttcgttg
    22981 aacgcctgcg gatcgttgag gaaacgacga gagatcttct cgaactcagg atcaaaacgc
    23041 agcgtcaggt cggtcaccag cattgtcggt ttacgtttct tcgacggatc gaacggatcc
    23101 gggataattt ccggtgcgtc taccgcttcg aactggattg ctccagccgg actgcgggtc
    23161 tgtacccact catacttgaa cagattctcg aagaaatagt tgctccactg gatcggcgtc
    23221 tgggtccaga ctacttccag accagaggta atggcatctg cgccaacgcc gctgccgtaa
    23281 gtgctcgccc agcctaaacc ttgttcttca atcggtgcag cttctggatc aggacctacg
    23341 tttgatgtcg gaccggcacc gtgggtttta cccagcgtat gaccacccgc aatcagcgcc
    23401 acggtttctt cgtcgttcat gcccatgttg ccgaaggtcg cgcggatagc tgctgccgca
    23461 gaaagcggtt cgccgctgtg atccgggcct tccgggttaa cgtaaatcag gcccatctcg
    23521 gttgcaccca gcggtgcttt cgccagcgct tccggatgac ggtgagtcag ccaggctttt
    23581 tcatcacccc agttaacatc cagatccggt tcccagacgt cttcacgacc ggcaccaaaa
    23641 ccgaaggtac ggaagccgga gttttccagc gccacgttac ctgcgaggat aaacaggtcg
    23701 gcccaggaga ttttctgacc atatttctgt ttgattggcc acaacaggcg acgcgcttta
    23761 tcgaggctta cgttatccgg ccaggagttc agcggtgcaa aacgttgctg accacgaccc
    23821 gcgccgccgc gtccatcgat tgaacggtaa gtacctgcgc cgtgccaagc catacgaata
    23881 aacagaccgg cgtagctgcc ccagtcggct ggccaccacg gctgagattc tgtcagcagg
    23941 gctttcagat cttttttcag gccgtagtaa tctaatttgc tgaattcttt gcggtagtca
    24001 aagtcctcac ccagcgggtt agaacgatta gaatgttggt ttaacaggtc aacacggagt
    24061 tgatttggcc accagtcgcg agtggttgtg cccgcccccg cgctctggtc gtgaccgccc
    24121 tgatggaacg ggcatttgcc agtggctgtg gtgttatgga tatcgtctga cgtgctcatc
    24181 aatgtgctcc cctctacagt gttaccgtta cgatacacag cgttagagag aagttataat
    24241 tgaattaatc cacggatgcg atagttgaga tcttacatat tgttggttaa agagatgtag
    24301 atcaaattga tcgtaattaa gcttttttga ttatgtccga attcggacaa atgctttata
    24361 aaaaagggtt ttagcttcat atccttcaag taatgaggga gaaaacaagg ctgacaacct
    24421 tattttcccg ccctcatttc gaggcagcat tttgtgctct gtttaaaatt tgtgatcact
    24481 gtgtgatttt cacaaaagcc acactattta taaaccaggt cgaacaccca gcgtatggca
    24541 aatcgcgtaa ctcatttcag cgcggttaag cgtatagaag tggaaatctt tcactccttc
    24601 acggcttaaa atcttcacca tatccatggc aatattcgcg cccaccagtt tgcgggtttc
    24661 ggcatcgtca tccagcccgt caaacatttg cgccatccac gccggaatac gcacgttggt
    24721 catgtcggca aatttcttcg cctgtttaaa gttagatacc ggcaaaatgc ccgggatgat
    24781 ttccacatca atgcccgccg atacacagcg gtcacgaaaa cgcaggtagc tttcgacatc
    24841 gaagaagaac tgagtaatcg cgcggttggc tccggcatcc actttgcgtt tcagattaag
    24901 caaatccgcc tgagcgcttt ttgcttccgg gtgaacttcc ggatacgccg ccacggatat
    24961 atcgaaatct gccacttctt ttaacagcgt caccaggtca gaagcataca tttctggctt
    25021 accacttccc ggcggcagat cgccacgcag tgccacgata tgacgaatac cgttattcca
    25081 gtagtcgcgt gcaatggtgc gcagctcgtc gggcgtcgca tcaatgcaag taagatgcgg
    25141 tgccgcttcc agaccagtgc gatctttaat gcctttaata atgctgtgcg tacggtcgcg
    25201 ctcgccggag ttcgcgccat aggtcaccga tacaaacttc ggtttcaggc tgctaaggcg
    25261 atcgatggag ttccacaggg tctgctccat ttcactggta cgcggcggga aaaactcgaa
    25321 cgaaacgtta atctgcccct ggacttctgc caggctctga ttcagggcat cccgctggct
    25381 ggcgtgaaaa aagctcatac cttacctcat caatcgcttg tagttatatg ttgtgttgtg
    25441 cacatctatc catttagacg tccagatgta aagatgaagg aaaagccgaa gggcgtcaac
    25501 cgaaaaatga ccaatgatgg tgaggaaaaa tcagggaaga tgaaaaaact tcaggatggt
    25561 aagaaaaaga aaatccgata tgcaaagcaa accggactgc aaatataaac taaccatgta
    25621 cttattgctt cttgccggat tcgtcgtaaa cgcttcatcc ggcctaattc tatgataatt
    25681 caatatattt cattagaaac catagacccg aaaacatgag taccgggcat tattaaattt
    25741 ctgaaattac aacaactgtg ccagccggtt gatatccgac tgaatcgccc cggcggtgac
    25801 gtcgcgccca gcgccaggcc cacggatcac cagcggatta tcgcggtacc agcggctttc
    25861 gatggcaaag acgttatcgc atggcagcag tgatgccagc ggatagtctt cacgcaccgc
    25921 ctccacgccc acacgcgctt tgccgttggc atcgaaacgc gccacgtagc gcagcaccag
    25981 ccccatttcg cgggccgctt ccagccgttg caccatctgc tcgttaagtt catcgccatt
    26041 ttcaaagaaa tggtcgatgc tgccgccttc gcaatgagca ggcaccagcg attccacacg
    26101 tacctggtcc ggttcaatgt tgtaacccgc ttcacgcgcc agaatcacca gcttgcgcat
    26161 tacgtctttg ccggagaggt cgtcacgcgg atcaggctcg gttaagccct gctgccacgc
    26221 ctgatccacc agctcggtaa acggcacgct accgtcgaat tgcaggaaca gccaggagag
    26281 cgtgccggag aagatcccac tgatcgacaa aatggtatcg ccgctgtcga tcagatcgcg
    26341 cacggtatgg ttgatcggca agcccgcacc aacggtggcg ttgtatagcc agtgacgccc
    26401 ggttttttcg aaggcgtcgt ggatctggcg atatttattg ctgtcgcttg ctcccgccag
    26461 tttgttggca ctgatgacgt ggaaaccgtg gctggcgaaa tcgagatact gatcagcaag
    26521 ctgctggctg gcggtaacgt ccaacaccac taaatcatca tacggatggg cgcgcatcca
    26581 caggaaaagc gactcttcat cctgctcgac cgcttcatcg ttgaagaagg ctaacgcgcg
    26641 gctggcgtcc agcccgtcat aactcaacag actgcggcgg ctgtccacca cgcctgccag
    26701 cacaaattca aagccggtac gcgccgaaag cgtgctctgc tcgcgggcga acagttccag
    26761 ccagcgagag ccgatattgc ctttgccgaa caataccagg ccgatgcgtt tttctgcgcg
    26821 gaagacggac tgatgcagcc cctgaatcag gctttcggtc gggccggtgc gcagtactgc
    26881 caccagactg atgccgtcat cggactgcca cgtaaactcg accggctggc ctttcagttg
    26941 ctgccagaag cggtggcaat gcagcgggtt acgggtgaca cccgccccga ccatcgccac
    27001 cagcgccagc ccctgacgca ggcgcagttc tccaggtaat cccgcttcgt cgaggatttt
    27061 cagcgcactg tcggccactt ctgaggtgta gcaaaattgc aacaactggc gatcgttatg
    27121 cacgccaacc gccagtggac gcacctgggc acgtttcagg atctggtcga tctctttatg
    27181 cgccagtttg aaatcctgac tggcgggcac ctgaaactca atcaaacaga catcatcgtg
    27241 gctggtgaca atacgcgcac cagtaccgga ggccagcacg cgttcaatgc gcgtggaacc
    27301 ttgatccggc gtgtagctac agcgcagctg caggtcgatt tcgctgccag aaactggctg
    27361 taaagtacgg gcgtgaagaa cgggagccgc cagacgcgcc agttcgctgg cctcatccag
    27421 acgcagcaac ggcagcaggc aggcatcttt cactttacgc gggtcggcac tgtatacccc
    27481 ggcgacgtcg ctccagatgg ttacacgaga aacacccgcc agcgcaccga tttgtgtcgc
    27541 ggaatagtcg gaaccgttac gccccagcaa caccgtttca ccggcgttgt tacggctgat
    27601 aaacccggtc accaccagac gtttgctcgg atgttgtacc agtagctgtt gcagcaacgg
    27661 gtaagaaagc ccttcatcaa cctgcggttg cgcagcgcgt tcagcgcgta aaaactcacg
    27721 ggcatcaagc caggccgctg gcagcccttg ttgattaagt accgcagaca tcaggcgcgc
    27781 cgaccacact tcaccgtggc ccaccacttc cgcatacacc gcatcgttaa taccactgtc
    27841 gagcaacgcc gccaggcgct caagatcgct gacaaaagcg ctaatgaggc tatcggcttc
    27901 ttcagcgggt agcagaccgc taatcagatc gcactgatag cgacgcagcg tttgttgaac
    27961 ctgatgcgca gagagacgat cggtctggct tagtttcaac cagttaatca actggttagt
    28021 ggtgctaccg gcggcggaaa ccaccatcat atcgtcaggc tgagagaact ccgccataat
    28081 gcccgcgaca cgcaaataac acttcacatc agccagacta ctgccaccaa atttatgcag
    28141 ctgacgacct ttcgcccctg cctgcgcaat cacactcatt tttacccctt gtttgcagcc
    28201 cggaagccat tttccaggtc ggcaattaaa tcttcgccat cttcaatacc ggtggagata
    28261 cgcagcagcg tctcggagat cccggcggca gcacgcgctt ctggtgccat gcctgcatgt
    28321 gtcatggttg cggcgtgaga gattaaactt tccactcccc ctaatgattc cgccagcgta
    28381 aacaacgaca gcccgcccag gaaacgacac agcgtctgct catcgccatc cagttcaaaa
    28441 ctcaacattg cgccaaagcc tttttgctga cgcgcggcaa tttcatgccc ctgattttcc
    28501 ggcagcgacg ggtgatacag ttttttcacc aacggctggg tttgcagata tttcacaatc
    28561 gcctgcgcgt tgcgctgcgc cagctccata cgcggcacca gcgttcgcaa cccgcgtagc
    28621 agcagatagc tgtcaaacgc gccgcccgtc acgccaatat tgtttgccca ccaggcaagt
    28681 tcagtgacaa cgtccgggtc tttagcaatc accacgccag ccaccacgtc agagtgaccg
    28741 ttcagatatt tcgtacatga atgcaacacc agatcggcac ccaatgccag cggattttgt
    28801 aatgccgggc ttaagaaggt gttatccacc acgctcaccg ccccgacttc ccttgccaga
    28861 tggcagattt tcgcaatatc cacgacgcgt aacaatggat tacttgggct ttctaccagt
    28921 accagtttgg gtttttctgc cagcgctgcc cgtaatgcct gttcatcgcc ttgatcaaca
    28981 aacaacacgc gatagcaacc gcgtttcgcc agactgtcga acaggcgata gctaccgccg
    29041 tagcagtcgt gcggcgcaac cagcagatcg ccaggtttca aaaagacggt cgttaccagg
    29101 tgaatcgcgg acatgccggt attagtaagt actgcgccag caccgccttc cagttctgcc
    29161 agcgcacgct gaaccacatc gcgcgttggg ttgccgcgac gcgagtaatc atgcgcgcgc
    29221 ggttcattaa atccggtaaa gttataggtg ctggaaagat ggatgggagg gacaacgcaa
    29281 ccatactgtt cgtcgtcatt taacccgcta cgcactgcga tggtggcctg tttacgcgtc
    29341 atgtgatgaa gtttcctggg ctttgtcggt gaaatgtcag gcaccagagt aaacattgtg
    29401 ttaatggacg tcaatacatc tggacatcta aacttctttg cgtatagatt gagcaaatcc
    29461 caaatagccg ttaaaattat atgcattatc acgccgacag gtgcattaca cgatgtcacg
    29521 gtaacgcctg tacggtaaac tatgcgggtt tacggtcagt acccacataa attgtggggt
    29581 ctggtctcaa tttattgacg aagaggatta agtatctcat ggctgaatgg agcggcgaat
    29641 atatcagccc atacgctgag cacggcaaga agagtgaaca agtcaaaaag attacggttt
    29701 ccattcctct taaggtgtta aaaatcctca ccgatgaacg cacgcgtcgt caggtgaaca
    29761 acctgcgtca cgctaccaac agcgagctgc tgtgcgaagc gtttctgcat gcctttaccg
    29821 ggcaaccttt gccggatgat gccgatctgc gtaaagagcg cagcgacgaa atcccggaag
    29881 cggcaaaaga gatcatgcgt gagatgggga ttaacccgga gacgtgggaa tactaaagcg
    29941 gaaaagagcg gcgcggagtg gaatcgcctg atgcactacg cttatcaggc ctacgtcata
    30001 ttgcaattta ttgaatttgc acgaacttgt aggccggata aggcgttcac gccgcatccg
    30061 gcataaacaa cgagcacgtt gtctgcgacc caccgctttt tatacatgga cgtttaacta
    30121 tgaaaaacag gctgctgatc ctcagcctgc tggtttctgt acctgccttt gcctggcagc
    30181 cgcaaactgg cgacatcatc tttcaaatct ctcgctcatc gcaaagtaaa gcgatccaac
    30241 tggcaaccca ttccgattac agccacaccg gtatgctggt gatgcgtaac aaaaagccct
    30301 acgtttttga agcggtcggc ccggtgaaat acaccccgct caagcagtgg attgcccatg
    30361 gtgaaaaggg caactacgtt gttcgccgcg ttgaaggcgg actgagtgtt gaacaacagc
    30421 aaaaactgac gcaaacggca aaacgttatc tcggtaaacc gtatgatttc agcttctcat
    30481 ggagcgacga tcgccagtac tgttcggaag tggtgtggaa ggtttaccag aacgcgctgg
    30541 gaatgcgcgt gggcgaacag cagaagttga aagagtttga tctcagcaac ccgctggtcc
    30601 aggcgaagct caaagaacgc tacggtaaaa atattccgct tgaggaaacg gtagtttcac
    30661 cacaagccgt tttcgacgcg ccacaactca ccacggttgc caaagagtgg ccgctgtttt
    30721 cgtggtgata cgaccaaaat tcaggcacaa aaaaagcgcc gtgcggcgct tttttcggaa
    30781 atccggtctt atttgctgcc cgggatgttg aaacgcttgt tgaagcggtc aacacggcca
    30841 ccggtagcaa catcacgctg tttgccagtg aagaacgggt ggcacttgct gcacacgtcg
    30901 aggttcaggt catgaccaac ggtggagcgg atttttatta cgttaccgca agagcagcta
    30961 gcagtaattt cttcgtattt cgggtgaata tcttttttca tgggaaaacc tcggtttaag
    31021 gccgcgtcgc tcttccagcc ctaacgccag acaccacgcg atgttaaaag tatagcttca
    31081 atacgatcat ttcgtacgaa ggcgcgaaat catacagaaa ttaaccagcg tatgcaaact
    31141 gatccgcact cttctacggc aatgtgtata ctaacccacc gaatttcaag tcaggatgat
    31201 gctatgcccg ttgcccacgt tgccttgccc gttccgcttc ctcgtacctt tgactatctg
    31261 ctgccagaag gcatgacggt taaagctggg tgtcgcgtgc gcgtgccgtt tggcaaacag
    31321 caggagcgca tcgggattgt ggtatcagtt agcgatgcca gcgaactgcc gctcactgag
    31381 ctaaaagcgg tagtcgaagt gctggatggt gagccggtgt ttactcactc cgtctggcga
    31441 ttgctgctat gggcggcaga ttactatcat catccgattg gcgatgtgct gtttcatgcc
    31501 ttgccgattt tactacgcca ggggcggcct gcggcgaacg cgccgatgtg gtactggttt
    31561 gccactgaac aaggtcaggc ggtggatctg aacagcctga aacgctcacc caagcaacaa
    31621 caggcgctgg cagcgttacg gcaaggcaaa atctggcgcg accaggttgc cacgctcgaa
    31681 tttaatgatg ccgcgttgca ggcgctgcgc aaaaaaggtc tgtgtgattt agcaagtgaa
    31741 acaccagagt ttagcgactg gcgaacgaac tatgccgttt ctggtgagcg gttgcgattg
    31801 aataccgaac aggccaccgc cgttggcgca attcatagcg cggcagatac tttttctgcc
    31861 tggctgctgg cgggcgttac cggttccggt aaaacggagg tttatctcag tgtactggaa
    31921 aacgtgctcg ctcagggcaa acaggcgctg gtgatggtgc cggaaatcgg cctgacaccg
    31981 caaactatcg cccgttttcg tgaacggttt aatgcgcccg tggaagttct gcattccggt
    32041 ctgaacgaca gcgagcgtct ttcggcgtgg ctgaaagcga aaaatggcga ggcggcgatt
    32101 gtgatcggca cccgctccgc gctgtttacg ccgtttaaaa atctcggcgt gattgtcatt
    32161 gacgaagagc acgacagttc ctacaagcag caggaaggct ggcgctatca tgcccgcgac
    32221 ctggcggtgt atcgtgcgca tagcgagcaa atcccgatta ttcttggctc cgcaacgccc
    32281 gcgctggaaa cgttgtgcaa cgtccagcag aaaaaatacc gcctgctgcg cctgacgcgc
    32341 cgggcgggga atgcgcgtcc ggcaattcaa cacgtgctgg atttaaaagg tcagaaggtg
    32401 caggcaggtc tggctccggc gttaatcacc cgtatgcgcc agcatttaca ggccaacaat
    32461 caggttatcc tctttcttaa ccgccgtggc tttgcgcctg cgctactgtg ccacgactgc
    32521 ggctggattg ccgaatgccc acgttgcgat cactactaca cgctgcatca ggcgcaacag
    32581 catttgcgct gtcaccactg tgacagtcag cgtccggtgc cgcgccagtg cccttcctgc
    32641 ggttccacgc acctggtacc cgtggggctg ggcaccgaac agcttgaaca gacgcttgcg
    32701 ccgctgttcc ctgatgtgcc catttctcgt atcgaccgcg ataccaccag ccgcaaaggg
    32761 gcgctggaac agcaactggc ggaagtacat cgcggcggtg cgaggatttt gatcggcaca
    32821 caaatgctgg cgaaaggtca ccactttccg gatgtgacgc tggtggcgtt actggatgtg
    32881 gacggcgcgc tgttttctgc cgattttcgc tcggcagagc gtttcgctca gctttacacc
    32941 caggtcgctg gtcgtgccgg gcgtgcgggt aaacaaggcg aagtggtgct gcaaacgcac
    33001 catccggaac atcctctgtt gcaaacgttg ctctataaag gctacgatgc ctttgccgaa
    33061 caggcgctgg ctgagcgacg gatgatgcaa ctgccgccat ggaccagcca tgtgattgtg
    33121 cgtgcggaag atcataacaa tcagcatgcg ccgctgttcc tgcaacaact gcgtaatctg
    33181 atcctctcca gcccgctggc agacgacaaa ctgtgggttc tcggtccggt tccggcactg
    33241 gcacctaaac gtggcggtcg ctggcgctgg cagatattgt tgcagcaccc ttcccgcgtg
    33301 cgcttgcaac acatcattag tggtacgctg gcgctcatca atacaatacc ggattcccgc
    33361 aaggtgaaat gggtactgga tgttgatccg attgagggtt aaaccgctta cgatgcgagg
    33421 cggatcgaaa aattcaatat tcatcacact tttcatgaaa attctgtaac cgttttcacg
    33481 cgctatctgc taaaaatgtt gccgatgtga agtaaacatg gatgtagtac gcctgacgtg
    33541 ccaggcgagg agtgagtgtg aaagcgaaga agcaggaaac tgccgcgacc atgaaagacg
    33601 ttgccctcaa ggcaaaagtc tctacagcga ctgtctcccg agcattaatg aatcccgata
    33661 aagtctccca ggccacccgt aatcgggttg aaaaagcggc ccgggaagtg ggttatttac
    33721 cgcagcctat ggggcgcaac gtcaagcgta atgaatcccg caccattctg gtgattgtcc
    33781 cggatatctg cgatcccttc tttagcgaaa ttattcgcgg tatcgaagtt acggcggcaa
    33841 atcacggata tctggtgctg attggcgact gtgcgcatca aaatcagcag gaaaaaacct
    33901 ttatcgattt gatcatcacc aagcaaattg atggcatgtt gctgctgggt tcaaggctgc
    33961 cgtttgatgc cagcattgag gaacagcgta atctgccgcc gatggtgatg gcgaacgaat
    34021 ttgcaccgga actggagctg cctaccgttc atatcgacaa tctgaccgcc gcatttgatg
    34081 cagtaaatta tttatatgag caagggcata aacggattgg ctgtatagcc ggtcccgaag
    34141 agatgccact gtgtcactac cgcctgcaag gctatgttca ggcgctgcgt cgctgcggaa
    34201 ttatggttga tccacaatac atcgcccgtg gcgacttcac cttcgaagcc ggaagcaaag
    34261 caatgcaaca gctgcttgat cttccacaac cgcctactgc agtcttctgc catagcgatg
    34321 tgatggcgct cggcgcactt tctcaggcaa aacgccaggg gctgaaagtc ccggaagacc
    34381 tttccataat cggttttgat aacatcgacc tgacgcaatt ttgtgatccg ccgctgacaa
    34441 ccatcgcgca gccgcgttac gaaatcggtc gggaagctat gctgttattg cttgatcaaa
    34501 tgcaggggca acacgttggc agtggctctc gtttaatgga ctgcgaactt atcatccggg
    34561 gatcaacacg cgcgttacct taaagtaaac ctttaagact gccgtgactg gtcaaaggcc
    34621 cgccgcttaa gtaacatggc gggctgacga acgaataaat acagcgaaac gatagtggca
    34681 caacgagatt atgtacgccg cagccaaccg gcaccttcgc ggcgaaaaaa gagcacctca
    34741 cggaaaaagc aacgaaatct gcctgcggtt tctcccgcta tggtcgctat tgctgccgcc
    34801 gttcttgtga cctttatcgg tggtctgtac ttcattacgc atcacaagaa agaagagtcc
    34861 gagacgctgc aaagccagaa agtgaccgga aacggactac caccaaaacc agaagaacgc
    34921 tggcgctaca ttaaagagct ggaaagtcgc cagccgggtg tgcgtgcgcc cacagaacct
    34981 tctgccggtg gtgaagtgaa aacgccggag caactgacac cagaacaacg tcagcttctt
    35041 gaacaaatgc aggctgatat gcgccagcag ccaacgcagc tggttgaggt tccgtggaat
    35101 gaacagacgc cggaacagcg tcagcaaacg ctacaacgcc agcgtcaggc gcagcaatta
    35161 gcggagcagc aacgcctggc tcagcagtcg cgtacgactg aacaaagctg gcagcagcag
    35221 acgcgtacgt cgcaagccgc gccggtacag gcgcaaccgc gccagtctaa accagcttct
    35281 actcagcagc cgtaccagga tctgctgcaa acccctgcgc acacgaccgc gcagtcgaag
    35341 ccgcagcaag ctgcgccggt tgctcgtgcc gctgacgcgc caaaaccgac ggcggagaaa
    35401 aaagacgaac gccgctggat ggtgcagtgc ggttcgttca gaggcgcgga acaggcagag
    35461 acggtacgtg ctcagctggc gttcgaagga tttgactcga aaatcaccac caacaatggc
    35521 tggaatcgtg tggtcattgg cccggtgaaa ggcaaagaga acgcagacag caccctcaat
    35581 cggttgaaga tggcgggtca tacaaactgc attcggctcg ccgccggggg ttgaaaccct
    35641 caaaatcccc cccatctata attgcattat gccccgtact tttgtacggg gtttgtactc
    35701 tgtattcgta accaaggggt cagctcgtga caactatagt aagcgtacgc cgtaacggcc
    35761 atgtggtcat cgctggtgat ggtcaggcca cgttgggcaa taccgtaatg aaaggcaacg
    35821 tgaaaaaggt ccgccgtctg tacaacgaca aagtcatcgc gggctttgcg ggcggtactg
    35881 cggatgcttt tacgctgttc gaactgtttg aacgtaaact ggaaatgcat cagggccatc
    35941 tggttaaagc cgccgttgag ctggcaaaag actggcgtac cgatcgcatg ctgcgcaaac
    36001 ttgaagcact gctggcagtc gcggatgaaa ctgcatcgct tatcatcacc ggtaacggtg
    36061 acgtggtgca gccagaaaac gatcttattg ctatcggctc cggcggccct tacgcccagg
    36121 ctgcggcgcg cgcgctgtta gaaaacactg aacttagcgc ccgtgaaatt gctgaaaagg
    36181 cgttggatat tgcaggcgac atttgcatct ataccaacca tttccacacc atcgaagaat
    36241 taagctacaa agcgtaagga tctcccatgt ctgaaatgac cccacgcgaa atcgtcagcg
    36301 aactggataa gcacatcatc ggccaggaca acgccaagcg ttctgtggcg attgctctgc
    36361 gtaaccgctg gcgtcgcatg cagctcaacg aagagctgcg ccatgaagtg accccgaaaa
    36421 atatcctgat gatcggcccg accggtgtcg gtaaaactga aatcgcccgt cgtctggcta
    36481 agctggcgaa tgcgccgttc atcaaagttg aagcgaccaa atttaccgaa gtgggctacg
    36541 tcggtaagga agttgattct attattcgcg atctgaccga tgccgccgtg aaaatggtac
    36601 gcgtccaggc tatcgagaaa aaccgttatc gcgctgaaga actggcagaa gaacgtattc
    36661 tcgacgtgct gatcccacct gctaaaaaca actggggaca gactgaacag cagcaggaac
    36721 cttccgctgc tcgtcaggca ttccgcaaaa aactgcgtga aggccagctt gatgacaaag
    36781 aaatcgagat cgatcttgcc gcagcaccga tgggcgttga aattatggct cctccgggca
    36841 tggaagagat gaccagccag ctgcagtcca tgttccagaa cctgggcggc cagaagcaaa
    36901 aagcgcgtaa gctgaaaatc aaagacgcca tgaagctgct gattgaagaa gaagcggcga
    36961 aactggtgaa cccggaagag ctgaagcaag acgctatcga cgctgttgag cagcacggga
    37021 tcgtgtttat cgacgaaatc gacaaaatct gtaagcgcgg cgagtcttcc ggtccggatg
    37081 tttctcgtga aggcgttcag cgtgacctgc tgccgctggt agaaggttgc accgtttcca
    37141 ccaaacacgg gatggtcaaa actgaccaca ttctgtttat cgcttctggc gcgttccaga
    37201 ttgcgaaacc gtctgacctg atcccggaac tgcaaggtcg gctgccaatc cgcgttgaac
    37261 tgcaggcact gaccaccagc gacttcgagc gtattctgac cgagccgaat gcctctatca
    37321 ccgtgcagta caaagcactg atggcgactg aaggcgtaaa tatcgagttt accgactccg
    37381 gtattaaacg catcgcggaa gcggcatggc aggtgaacga atctaccgaa aacatcggtg
    37441 ctcgtcgttt acacactgtt ctggagcgtt taatggaaga gatttcctac gacgccagcg
    37501 atttaagcgg tcaaaatatc actattgacg cagattatgt gagcaaacat ctggatgcgt
    37561 tggtggcaga tgaagatctg agccgtttta tcctataatc gcgttcaatc attttcatca
    37621 ttgtttgatg gggctgaaag gccccatttt tattggcgcg tattatgact gaacaacaaa
    37681 ttagccgaac tcaggcgtgg ctggaaagtt tacgacctaa aaccctcccc ctcgcctttg
    37741 ctgcaattat cgtcgggacg gcgctggcat ggtggcaagg tcacttcgat ccgctggtcg
    37801 ccctgctggc actaattacc gccgggctat tacagatcct ttctaacctc gccaatgatt
    37861 acggcgatgc ggtaaaaggc agcgataaac ctgaccgcat tgggccgcta cgcggcatgc
    37921 aaaaaggggt cattacccag caaaagatga aacgggcgct cattattacc gtcgtgctca
    37981 tctgtctctc cgggctggca ctggttgcag tggcatgcca tacgctggcc gattttgtcg
    38041 gtttcctgat tcttggcggg ttgtcgatca ttgccgctat cacctacacc gtgggcaatc
    38101 gtccttatgg ttatatcggt ctgggtgata tttccgtact ggttttcttt ggctggttga
    38161 gtgtcatggg gagctggtat ttacaggctc atacattgat tccggcactg atccttccgg
    38221 cgaccgcatg cggcctgctg gcaacggcag tactgaatat taataacctg cgtgatatca
    38281 atagcgaccg cgaaaatggc aaaaacacgc tggtggtgcg cttaggtgaa gtgaacgcgc
    38341 gtcgttatca tgcctgcctg ctgatgggct cgctggtgtg tctggcgctg tttaatctct
    38401 tttcgctgca tagcctgtgg ggctggctgt tcctgctggc ggcaccatta ctggtgaagc
    38461 aagcccgtta tgtgatgcgg gaaatggacc cggtggcgat gcgaccaatg ctggaacgta
    38521 ctgtcaaggg agcgttactg actaacctgc tgtttgtttt agggatattc ctaagccagt
    38581 gggcagcata actgacaaat atcaattaac aattgatgat tttgccaaca gcccacatag
    38641 cgcgatatac tgaaaattct cgcagcaact gaatgttaag cctatgaaat acgatacttc
    38701 cgagctttgt gacatctatc aagaagatgt taacgtcgtg gaaccgctgt tctccaactt
    38761 tggcggacgg gcgtcgtttg gcggacaaat aatcacggta aaatgtttcg aggacaacgg
    38821 gttgctgtac gatctgctcg aacagaatgg ccgtggtcgt gttcttgtcg tcgatggcgg
    38881 tggttctgtt cgtcgcgcac tggtcgatgc tgaactggcg cgtctggcag tacaaaatga
    38941 atgggaaggt ctggtcattt acggcgcggt gcgtcaggta gatgacctgg aagagttgga
    39001 tatcggcatc caggcgatgg cggcaattcc ggttggtgcc gctggcgaag gcattggcga
    39061 aagcgatgtc cgcgtcaatt ttggcggtgt caccttcttc tccggcgacc atctttatgc
    39121 cgacaatacc gggattattc tttcagaaga tccgctggat attgaatgat aagaaaggca
    39181 ccgcaaggtg ccttttttct gcgttacctg ttggcctaca cagtaaagaa attacgcgaa
    39241 agatgaagcg taatcagacc tcttccatgc gacccagcag ggcctgcaga cgttcctgcc
    39301 agccgttctg ctgttctttc agatggttgt tctcacgctc cagctcttcg cgctgatgct
    39361 gggcattttg aacttcctgc gacagtgagt tgtttttttc tttcagctct tcgatttcca
    39421 tctgcaacag agtgatggta tcaatcgcct gctgtacttt tgcttccagt ttctcaaaca
    39481 cttctaatga cattgtcata cctctcctga attgcaaggc gttgatggat aaaaaatcct
    39541 cgtcccgatt accggtgacg ccttaataaa tacgagcgca ctttagttag ctccgattgt
    39601 atgaagccgc gccatcgctg tccagcggca cgccttgcag attacggttt gccacacttt
    39661 tcatccttct cctagtgaca taatccacat caatcgaaaa tgttaataaa tttgttgcgc
    39721 gaatgatcta acaaacatgc atcatgtaca atcagatgga ataaatggcg cgataacgct
    39781 cattttatga cgaagcacac acattttaag ttcgatattt ctcgtttttg ctcgttaacg
    39841 ataagttaac agcatgccta caagcatcgt ggaggtccgt gactttcacg catacaacaa
    39901 acattaactc ttcaggatcc gattatgagt caaacatcaa ccttgaaagg ccagtgcatt
    39961 gctgaattcc tcggtaccgg gttgttgatt tttttcggtg tgggttgcgt tgcagcacta
    40021 aaagtcgctg gtgcgtcttt tggtcagtgg gaaatcagtg tcatttgggg actgggggtg
    40081 gcaatggcca tctacctgac cgcaggggtt tccggcgcgc atcttaatcc cgctgttacc
    40141 attgcattgt ggctgtttgc ctgtttcgac aagcgcaaag ttattccttt tatcgtttca
    40201 caagttgccg gcgctttctg cgctgcggct ttagtttacg ggctttacta caatttattt
    40261 ttcgacttcg agcagactca tcacattgtt cgcggcagcg ttgaaagtgt tgatctggct
    40321 ggcactttct ctacttaccc taatcctcat atcaattttg tgcaggcttt cgcagttgag
    40381 atggtgatta ccgctattct gatggggctg atcctggcgt taacggacga tggcaacggt
    40441 gtaccacgcg gccctttggc tcccttgctg attggtctac tgattgcggt cattggcgca
    40501 tctatgggcc cattgacagg ttttgccatg aacccagcgc gtgacttcgg tccgaaagtc
    40561 tttgcctagc tggcgggctg gggcaatgtc gcctttaccg gcggcagaga cattccttac
    40621 ttcctggtgc cgcttttcag ccctatcgtt ggcgcgattg taggtgcatt tgcctaccgc
    40681 aaactgattg gtcgccattt gccttgcgat atctgtgttg tggaagaaaa ggaaaccaca
    40741 actccttcag aacaaaaagc ttcgctgtaa tatgactacg ggacaattaa acatgactga
    40801 aaaaaaaata tcgttgcgct cgaccagggc accaccagct cccgcgcggt cgtaatggat
    40861 cacgatgcca atatcattag cgtgtcgcag cgcgaatttg agcaaatcta cccaaaacca
    40921 ggttgggtag aacacgaccc aatggaaatc tgggccaccc aaagctccac gctggtagaa
    40981 gtgctggcga aagccgatat cagttccgat caaattgcag ctatcggtat tacgaaccag
    41041 cgtgaaacca ctattgtctg ggaaaaagaa accggcaagc ctatctataa cgccattgtc
    41101 tggcagtgcc gtcgtaccgc agaaatctgc gagcatttaa aacgtgacgg tttagaagat
    41161 tatatccgca gcaataccgg tctggtgatt gacccgtact tttctggcac caaagtgaag
    41221 tggatcctcg accatgtgga aggctctcac gagcgtgcac gtcgtggtga attgctgttt
    41281 ggtacggttg atacgtggct tatctggaaa atgactcagg gccgtgtcca tgtgaccgat
    41341 tacaccaacg cctctcgtac catgttgttc aacatccata ccctggactg ggacgacaaa
    41401 atgctggaag tgctggatat tccgcgcgag atgctgccag aagtgcgtcg ttcttccgaa
    41461 gtatacggtc agactaacat tggcggcaaa ggcggcacgc gtattccaat ctccgggatc
    41521 gccggtgacc agcaggccgc gctgtttggt cagttgtgcg tgaaagaagg gatggcgaag
    41581 aacacctatg gcactggctg ctttatgctg atgaacactg gcgagaaagc ggtgaaatca
    41641 gaaaacggcc tgctgaccac catcgcctgc ggcccgactg gcgaagtgaa ctatgcgttg
    41701 gaaggtgcgg tgtttatggc aggcgcatcc attcagtggc tgcgcgatga gatgaagttg
    41761 attaacgacg cctacgattc cgaatatttc gccaccaaag tgcaaaacac caatggtgtg
    41821 tatgtggttc cggcatttac cgggctgggt gcgccgtact gggacccgta tgcgcgcggg
    41881 gcgattttcg gtctgactcg tggggtgaac gctaaccaca ttatacgcgc gacgctggag
    41941 tctattgctt atcagacgcg tgacgtgctg gaagcgatgc aggccgactc tggtatccgt
    42001 ctgcacgccc tgcgcgtgga tggtggcgca gtagcaaaca atttcctgat gcagttccag
    42061 tccgatattc tcggcacccg cgttgagcgc ccggaagtgc gcgaagtcac cgcattgggt
    42121 gcggcctatc tcgcaggcct ggcggttggc ttctggcaga acctcgacga gctgcaagag
    42181 aaagcggtga ttgagcgcga gttccgtcca ggcatcgaaa ccactgagcg taattaccgt
    42241 tacgcaggct ggaaaaaagc ggtcaaacgc gcgatggcgt gggaagaaca cgacgagtaa
    42301 tgtcaggcac ctgaatcgat tgcctgatgc tgatcgcctg atgcgacgct tacgcgtctt
    42361 atcaggccta cgagccaatg ccattttgtg taggcgggat aaggcgttca cgccgcatcc
    42421 ggcatcacgc agactctgaa accgtgcggg ggcaaccccg cacacatcaa taatcccacc
    42481 cttcccctgt gctacacttc gcgccattcc ttactgctta gagtttgcta tgagacgaga
    42541 acttgccatc gaattttccc gcgtcaccga atctgcggcg ctggctggct acaaatggtt
    42601 aggacgcggc gataaaaaca ccgcggacgg cgcggcggta aacgccatgc gtattatgct
    42661 caaccaggtc aacattgacg gcaccatcgt cattggtgaa ggtgaaatcg acgaagcacc
    42721 gatgctctac attggtgaaa aagtcggtac tggtcgcggc gacgcggtag atattgctgt
    42781 tgatccgatt gaaggcacgc gcatgacggc gatgggccag gctaacgcgc tggcggtgct
    42841 ggcagtaggc gataaaggct gcttcctcaa tgcgccggat atgtatatgg agaagctgat
    42901 tgtcgggccg ggagccaaag gcaccattga tctgaacctg ccgctggcgg ataacctgcg
    42961 caatgtagcg gcggcgctcg gcaaaccgtt gagcgaactg acggtaacga ttctggctaa
    43021 accacgccac gatgccgtta tcgctgaaat gcagcaactc ggcgtacgcg tatttgctat
    43081 tccggacggc gacgttgcgg cctcaattct cacctgtatg ccagacagcg aagttgacgt
    43141 gctgtacggt attggtggcg cgccggaagg cgtggtttct gcggcggtga tccgcgcatt
    43201 agatggcgac atgaacggtc gtctgctggc gcgtcatgac gtcaaaggcg acaacgaaga
    43261 gaatcgtcgc attggcgagc aggagctggc acgctgcaaa gcgatgggca tcgaagccgg
    43321 taaagtattg cgtctgggcg atatggcgcg cagcgataac gtcatcttct ctgccaccgg
    43381 tattaccaaa ggcgatctgc tggaaggtat tagccgcaaa ggcaatatcg cgactaccga
    43441 aacgctgctg atccgcggca agtcacgcac cattcgccgc attcagtcca tccactatct
    43501 ggatcgcaaa gacccggaaa tgcaggtgca cattctctga gtgatttgat cgattgagcc
    43561 ttccagtcct tcgggactgg aatttttttg ttcggagaac gaagataagg caagtcaatc
    43621 aaaacaggag aaaaacatgg ctgattgggt aacaggcaaa gtcactaaag tgcagaactg
    43681 gaccgacgcc ctgtttagtc tcaccgttca cgcccccgtg cttccgttta ccgccgggca
    43741 atttaccaag cttggccttg aaatcgacgg cgaacgcgtc cagcgcgcct actcctatgt
    43801 taactcgccc gataatcccg atctggagtt ttacctggtc accgtccccg atggcaaatt
    43861 aagcccacga ttggcggcac tgaaaccggg cgatgaagtg caggtggtta gcgaagcagc
    43921 tggcttcttt gttctggatg aagtaccgga ctgcgaaacg ctatggatgc tggcaaccgg
    43981 tacagcgatt ggcccttatt tatcgattct gcaactaggc aaagatttag atcgcttcaa
    44041 aaatctggtc ctggttcacg ccgcacgtta tgccgccgac ttaagctatt tgccactgat
    44101 gcaagaactg gaaaaacgct acgaaggcaa actgcgcatt cagacggtgg tcagtcggga
    44161 aacggcagcg gggtcgctca ccggacggat accggcatta attgaaagtg gggaactgga
    44221 aagcgcgatt ggcctgccga tgaataaaga aaccagccat gtgatgctgt gcggcaatcc
    44281 acagatggtg cgcgatacac aacagttgct gaaagagacc cggcagatga cgaaacattt
    44341 acgtcgccga ccgggccata tgacagcgga gcattactgg taagcggtta cttatcgata
    44401 aacggcacga tgagcaaatc cgcactcatc ttattgatca tcccgcgata tgccggcatc
    44461 aaacggttga taaatgagtg atgatgacca cagacaagga ggtcgcactg ctctttttgc
    44521 ataatttcca gcagtgtttc cggcatttct ccgcgttcaa tacgcagttt tgtcttcggc
    44581 cattgaatat ttttcgtcag tttatacagc ttgttatccg acttattctt caacaattga
    44641 agaatatctt ctgttgcagg gaagtagata cccggataca actcgcttaa gccatcatca
    44701 atatgaatta acgtcaggtg agcgtcatta tgtctggcga gctccagggc tttattcacc
    44761 agtaaggcat cttcttcatt cccggaaatt gccacgccaa tgtgtttata agccatgttt
    44821 aactccttct aaagcaactc catcaagcta acgaacgcag tgataactca aaaataatca
    44881 tctcggcctg gcatgagaaa gtgaaggccg catcaagctc aacttgcccc tccagatcct
    44941 taatcttata ggccacgctg gccccttcat ctgcagagga tgcagcggcg gcaagctctt
    45001 tcagtttggt cataaaagac tctgcttctt cgcgggtggc aaaaaagcgc gaaaacttac
    45061 tggtacagtt gtcgttatca atcaccgtac cgatatcaat cgcacatcct ttagtactgc
    45121 atttatctac gacatctttc atagggcacc tctgtatatt cgcctctctg tcgattacga
    45181 cttaagaata atgcgccgtt aagggaagaa caaacaagta atgatcttat ggattaccat
    45241 tttttttgat attgcttaag gtgaagagtt cagaaggatt ttccgggtat tgtcatttgc
    45301 atcgtggaaa taacaatccc ggaaaaaaat agcagtaaga gatatattaa aggttaatta
    45361 cttgatttat tgtcggcttt atacttcaca tcctgagtat ctttaccata tttattttcg
    45421 ccttgtgtgc caacaaacgc accaagatcg ataagcatca tcaccagaat caacgtcggg
    45481 acaaaacgcc ccaccgccca ttgccagaca cccggtaaaa tcgctcagtt acccgccagc
    45541 agcatccacg ccacaatcat cagaaatgcc catgcgccgg aacgcccgcg atcatgcaag
    45601 cgcttaacag ttactgccgc tgttggccag agcaagcaca caaggcaaaa cgccgcggtc
    45661 tgaatatcga gtagattctt acccgccagt gagaaaagca ccagcatgcc tgcgaaccac
    45721 aggcctatcc aaatccagaa atcacggcgt ccaatacgcc ctttaaatga gaataaccat
    45781 tgctgtatgg tcatgtaagt tccttgatgg ttgtcttttc caggattcta cccttttgac
    45841 aagggcgaca gatatcgttt taatcggagc cagtcataac aaaaggtact gtcaatgaag
    45901 ccagggtgta cgctgttttt tctcttatgt tctgcattaa ccgttacaac aacggcgcat
    45961 gcacaaacac cagatacggc aacgaccgcg ccttatctgc tggctggagc ccctactttc
    46021 gatctctcca tcagccagtt tcgagaagac tttaacagcc agaatcccag cctgccactg
    46081 aacgaatttc gtgccatcga cagcagtccc gacaaagcca atctcactcg tgctgcaagt
    46141 aaaattaacg agaacttgta tgcttctaca gcgctggagc gcggtacctt aaaaatcaaa
    46201 agcattcaaa tgacctggct acccatccag gggccagagc aaaaagcagc gaaagcgaaa
    46261 gctcaggaat acatggcagc agtgatccgc acactcaccc cattaatgac caaaacacaa
    46321 agccagaaaa aactgcagtc gctactaacg gcggggaaaa acaaacgtta ttacaccgag
    46381 acagaaggtg cactgcgtta tgttgtcgcg gacaacggcg aaaaggggct gaccttcgct
    46441 gttgaaccga ttaagctggc gctatctgaa tcgcttgaag gtttgaataa atgacaaaaa
    46501 gcaaagcctt tgtgccgatg aatctctata ctgtttcaca gacctgctgc cctgcggggc
    46561 ggccatcttc ctttattcgc ttataagcgt ggagaattaa aatgcgacat cctttagtga
    46621 tgggtaactg gaaactgaac ggcagccgcc acatggttca cgagctggtt tctaacctgc
    46681 gtaaagagct ggcaggtgtt gctggctgtg cggttgcaat cgcaccaccg gaaatgtaca
    46741 tcgatatggc gaagcgcgaa gctgaaggca gccacatcat gctgggtgcg caaaacgtgg
    46801 acctgaacct gtccggcgca ttcaccggtg aaacttctgc cgctatgctg aaagacatcg
    46861 gcgctcagta catcatcatc ggccactctg aacgtcgtac ttaccacaaa gagtctgacg
    46921 aactgattgc gaaaaaattc gcggtgctga aagagcaggg tctgactccg gttctgtgca
    46981 tcggtgaaac cgaagctgaa aacgaagcgg gaaaaactga agaagtttgc gcacgtcaga
    47041 tcgacgcggt actgaaaact cagggtgctg cggcattcga aggtgcggtt atcgcttacg
    47101 aacctgtatg ggcaatcggt actggcaaat ctgcaactcc ggctcaggca caggctgttc
    47161 acaaattcat ccgtgaccac atcgctaaag ttgacgctaa catcgctgaa caagtgatca
    47221 ttcagtacgg cggctctgta aacgcgtcta acgctgcaga actgtttgct cagccagaca
    47281 tcgacggcgc gctggttggc ggtgcttctc tgaaagctga cgctttcgca gtgatcgtta
    47341 aagctgcaga agcggctaaa caggcttaag tctgacaggt gccggatttc atatccggca
    47401 cttactttcc ttaactcctc gccttaacgc aaaatctcac actcatgatc ctggatttcc
    47461 tcggctgaag cacggttcag cgtcagtaga tttcgttgtg tcgccagcaa cacaaacgaa
    47521 ttatcactct gccgtaccat cgccagcccg tagcttccca tatgttcccg cgcctcaggc
    47581 acttcttctg caagcatcat aaatgggctg cgttgtacca gttcgctttc cgttacccga
    47641 cgcgccaggt attcatgccc gcgcaaacca cctggcagtg gcaaccagcg gctgctgatg
    47701 ttcgccagat ttttatccag ttgttcgcgc acatcaggac gaatacaaga gatatgaata
    47761 tgaaaatggt tttgcgtatg cccggtgcgg gagttgatcg ccaaagaaac cgcgcgatcg
    47821 ggaaccggct ggccgtattt tttgctcata aaatcacgcg cctgccaggc caaccaaaag
    47881 aagttcggcg ttgaaggatc ggtcaacaaa ggactttcag taccgttaat acgatacgtt
    47941 ggcatcaaca gatattgcag tggaccatta agatctttta aaaccacgta tccggcattg
    48001 ggtttgactt ccgcacatgg cgaaggattt tgattttgct gctgattggg caaacattcc
    48061 tcaaggacaa tcttacgtaa tgtatccgac tcttcaccgg ttaatttcca gtaaccaata
    48121 ccggcagcca caacggcgat aactatcatc accaaaaaaa gaagacccgc ttttttcatc
    48181 tttttttccc tgtacctcaa agagatgcaa gggtaacgca aaatcgtgac aaataaaaaa
    48241 cccggtcgcg agaaaccacc gggtttgata attatcctag ggaaatcagc gtttgctgat
    48301 ctgatcgaac gtaccgccgt tagcaaaatg ctctttttgc gctttcgtcc agccgccgaa
    48361 ctcttcatca atggtgaata acttcagctt tggaaacgca ttttcgtact ttttcgctac
    48421 ttcagcgtcg cgcggacggt agtagttttt cgcggcaatt tcctgacctt ctggcgagta
    48481 gagatatttc aggtaggctt ccgccacctc tttggtgcct tttttctcga ccactttatc
    48541 gaccaccgac accgttggct ctgcgaggat agactcactc ggcgtgacga tttcgaattt
    48601 atctttcccc agttcattcg ctgccagcag agcttcgttt tcccaggcga tcagtacatc
    48661 gccaatcccg cgctcgacaa aggtgttagt ggagccgcgc gcgccagaat ccagaacttc
    48721 gacgttttta tacagtgccc gaacaaaatc ctgtgctttt gcctgatcgt tgttgttgtg
    48781 atgcagcgcg tagccccagg cagccaggta gttccagcgt gcgccaccag agcttttcgg
    48841 attaggcgtg atcaccgaaa cacccggttt aatcagatcg ttccagtcat ggatctgctt
    48901 cggattgccc ttacgcacca ggaaaacaat ggtggaagtg tacggtgcgg agttatccgg
    48961 cagacgtttg atccactctt tatcaatccg cccgcgttcc gcaatcgcgt ccacgtcata
    49021 ggccagagcc agcgtgacaa catcagcttc aataccgttg attaccgacg tcgcttgttt
    49081 acctgaacca ccgtgtgact gacgaatcac cacgttatca ccagtttgct gtttccagtg
    49141 ggcgctgaat gccttgttgt actgttcgta caattcgcgc gttggatcat atgaaacgtt
    49201 aagaagctga atatcctttg ccataacgct ggttgccgcc agcaaaaatg ttaaccctac
    49261 gccccacttg ttcatcgccc gactctctta tgttgtgttg tgatgagcaa agcgtgccag
    49321 aaggttaacc aaacattaaa gaataaaaaa agattggcta taacttgcgg gtatatgttg
    49381 agggattaaa aaggcggaag aaatccgcct catattgctg acaaagtgcg ctttgtccat
    49441 gccggatgcg gcgcgaacgc cttatccggc ctacaaaagt ttgcaaattc aataaattgc
    49501 agaattcatg taggcctgat aagcgaagcg catcaggcat ttttgcttct gtcatcggtt
    49561 tcaggctaaa ggaatctgcc tttttccgaa atcattaata cagttttttc gcgcagtcca
    49621 gccagtcacc tttgaacgga cgcttcatgt tttcgatagc gtcgatgatg tcgtggtgaa
    49681 ccagctgttc gttctggata cctacacaac gaccgccgta acctgccagc agcagatcga
    49741 tagcgtaagc gcccatacgg gaagccagaa tacggtcgta aggcaccgga gaaccaccgc
    49801 gctggatgtg gcccagcaca gttgcgcggg tttcacgacc ggtttctttc tcgatgaaat
    49861 gcgccagttc gtcaacatca cacatatgtt cggtaatcgc cacgatcgcg tgttttttac
    49921 ctttcgcgat acccgctttg atttcgttta ccaggtcttc acggctgaat tcaacttccg
    49981 gaactacaac gaattcacag ccaccggcaa tggccgcagc cagggtcaga tcgccacaat
    50041 aacggcccat cacttccacc acggaaatac gctggtgaga agaagaggtg tcacgcagac
    50101 ggtcgatcgc ttctacaacg gtgctcagcg cagtgaagaa accgatagtg tagtcagtgc
    50161 ctttgatgtc gttgtcgata gtgcccggca gaccgatgca cgggaagccc atttcggtca
    50221 gacgcattgc acccatgtag gaaccgtcac cgccgataac caccagcgcg tcgataccac
    50281 gttttttcag gttttcgata gccacggcgc ggatgttctc gtcgcggaat tccgggaaac
    50341 gcgcagaacc gaggaacgta ccgccacggt tgatcatgtc agacacgctg taacggtcta
    50401 gctgtaccat acggtcttca tacagaccca gatagccgtc ataaataccc attacttcca
    50461 gaccttctgt cagcgcagaa cgaacaaccc cgcgaattgc ggcgttcatg cctggcgcat
    50521 caccgccgct tgtcaacaca cctattttct taatcatgac tacctctgaa ctttggaatg
    50581 caaaatgaaa tctgttgccg gaagtcttct tgcacatcga agtgatccaa cgaatgtgca
    50641 aatagtataa caatcacttc ctgctgaatt gattcaggtc aggccaaatg gcggtatttt
    50701 atacacaaaa tgcgggtctg gctctctttt atactgatta tgaaagcata gaccgtttac
    50761 cctccctggg tacgacggaa caggggtcct gatggataat cacatccgat cccggaaaac
    50821 gccgtaaaat agcctgctct acctgatccg ccaccatatg tgcctgaacc aaaggcagag
    50881 agtcttccat ttccaaatga atctgaataa agcgggtcgg ccctgactgc cgcgtgcgaa
    50941 gatcgtgagc gccgctaaca cccggccagg aagtcacgat atcaataatt tcttgccgtt
    51001 cttcatcagg caatgcgcga tccagtaatg actgtaccgc ctcatatccc atgcgtaacg
    51061 cgctatataa aatatagatg ccgattccca atgcaaacag agcatcggcg cgatgccagc
    51121 cgtaccaaga caaccccagc gccagcagaa ttgcgccgtt catcataaca tcagactggt
    51181 aatgtagcat atcagcccgc accgcctggc tttgcgtccg gcgcaccacc caccgctgaa
    51241 acgagacaag gataatcgta caaattagcg ccacaattgt cacgataacc ccgacgcctg
    51301 gatctgtcat cggtgttgga gataccagat gttgaatacc cgtcaaaaac aggaatagtg
    51361 ccgaaccgga gataaacata ctttgcgcca gcgccgcgag ggactcagct ttaccgtgac
    51421 caaacgagtg attatcgtcg gcaggttgca gggaatatcg caccaccagt aaattcgtca
    51481 acgacgcgcc gatatccacc agcgaatcca ccagcgcggc gagaatactc accgacccgg
    51541 tataccacca tgcaaaaatt ttaatcagca atagcaacga agccatcgcc gtcgcagcaa
    51601 tcgccgcccg actgaccagc cgtccataag attgattcat aaatactccc gctatcaact
    51661 gacgctagta taacggaagc aaatcatctg caatgcatta agcagcaggc aaattgagga
    51721 taaaaaaaac cccacatcat gtgggggaag acagggatgg tgtctatggc aaggaaaaca
    51781 gggtttacta ctgggaacgt gagttgctac tactcaatag cttcaacgat gaactttttt
    51841 gccattgcgt cacgtcacgc aactgctcca ttcgttgttg atgtttctcg tttaaaaccg
    51901 cttgctgctc cggcgttaac aggcgataca tttggttgcg gacttttgcc atctcaacct
    51961 gacgagcaat ttgctcattc gccatttttt ctgcctgtgc gcgcacagcg ttttcatcaa
    52021 aattttctgc ggtgacaagg cgatgcattg tctccagttc gctaacatta acaggaggct
    52081 gttcgtgccg ggcctgttgc ataagatctc gcatctgctg acgctgatgt tcggttaaac
    52141 ttatgccgtc gaacatatgg ctctgcgtac tgcgctgcgt aagttcttca cccggatgcc
    52201 agttatcgcc tgaaccgact tcagcagcgt ggcttaatga actgactgcc agcgttgagg
    52261 ccatgacggc agcggtaact atgcgcatca tttgctccca aaatctttct gtcgcgattc
    52321 aacgatagag agtttacgat tcaggctgca aacatgcgtc agggggtgta aaacaacgta
    52381 aagtcatgga ttagcgacgt ctgatgacgt aatttctgcc tcggaggtat ttaaacaatg
    52441 aataaaatcc tgttagttga tgatgaccga gagctgactt ccctattaaa ggagctgctc
    52501 gagatggaag gcttcaacgt gattgttgcc cacgatgggg aacaggcgct tgatcttctg
    52561 gacgacagca ttgatttact tttgcttgac gtaatgatgc cgaagaaaaa tggtatcgac
    52621 acattaaaag cacttcgcca gacacaccag acgcctgtca ttatgttgac ggcgcgcggc
    52681 agtgaacttg atcgcgttct cggccttgag ctgggcgcag atgactatct cccgaaaccg
    52741 tttaatgatc gtgagctggt ggcacgtatt cgcgcaatcc tgcgccgttc gcactggagc
    52801 gagcaacagc aaaacaacga caacggttca ccgacactgg aagttgatgc cttagtgctg
    52861 aatccaggcc gtcaggaagc cagcttcgac gggcaaacgc tggagttaac cggtactgag
    52921 tttaccctgc tctatttgct ggcacagcat ctgggtcagg tggtttcccg tgaacattta
    52981 agccaggaag tgttgggcaa acgcctgacg cctttcgacc gcgctattga tatgcacatt
    53041 tccaacctgc gtcgtaaact gccggatcgt aaagatggtc acccgtggtt taaaaccttg
    53101 cgtggtcgcg gctatctgat ggtttctgct tcatgatagg cagcttaacc gcgcgcatct
    53161 tcgccatctt ctggctgacg ctggtgctgg tgttgatgtt ggttttgatg ttacccaagc
    53221 tcgattcacg ccagatgacc gagcttctgg atagcgaaca gcgtcagggg ctgatgattg
    53281 agcagcatgt cgaagcggag ctggcgaacg atccgcccaa cgatttaatg tggtggcggc
    53341 gtctgttccg ggcgattgat aagtgggcac cgccaggaca gcgtttgtta ttggtgacca
    53401 ccgaaggccg cgtgatcggc gctgaacgca gcgaaatgca gatcattcgt aactttattg
    53461 gtcaggccga taacgccgat catccgcaga agaaaaagta tggccgcgtg gaactggtcg
    53521 gtccgttctc cgtgcgtgat ggcgaagata attaccaact ttatctgatt cgtccggcca
    53581 gtagttctca atccgatttc attaacttat tgtttgaccg cccgctatta ctgctgattg
    53641 tcaccatgtt ggtcagtacg ccgctgctgt tgtggttggc ctggagtctg gcaaaaccgg
    53701 cgcgtaagct gaaaaacgct gccgatgaag ttgcccaggg aaacttacgc cagcacccgg
    53761 aactggaagc ggggccacag gaattccttg ccgcaggtgc cagttttaac cagatggtca
    53821 ccgcgctgga gcgcatgatg acctctcagc agcgtctgct ttctgatatc tctcacgagc
    53881 tgcgcacccc gctgacgcgt ctgcaactgg gtacggcgtt actgcgccgt cgtagcggtg
    53941 aaagcaagga actcgagcgt attgaaaccg aagcgcaacg tctggacagc atgatcaacg
    54001 atctgttggt gatgtcacgt aatcagcaaa aaaacgcgct ggttagcgaa accatcaaag
    54061 ccaaccagtt gtggagtgaa gtgctggata acgcggcgtt cgaagccgag caaatgggca
    54121 agtcgttgac agttaacttc ccgcctgggc cgtggccgct gtacggcaat ccgaacgccc
    54181 tggaaagtgc gctggaaaac attgttcgta atgctctgcg ttattcccat acgaagattg
    54241 aagtgggctt tgcggtagat aaagacggta tcaccattac ggtggacgac gatggtcctg
    54301 gcgttagccc ggaagatcgc gaacagattt tccgtccgtt ctatcggacc gatgaagcac
    54361 gcgatcgtga atctggcggt acaggattgg ggctggcgat tgttgaaacc gccattcagc
    54421 agcatcgtgg ctgggtgaag gcagaagaca gcccgctggg cggtttacgg ctggtgattt
    54481 ggttgccgct gtataagcgg agttaaactc cgcatttgta ggcaggataa ggcgtttacg
    54541 ccgcatccgg catttgagca ggatgcctga tgcgacgctg atagcgtctt atcaggccta
    54601 cactccgggg gttattttcc ccacaaccgg cgggaaaaat cttcgatctt gccgctcagt
    54661 cggcgctttt gcatcgtccg cgtccagctt ttcgataacc cggcggcgga gagtaaacgg
    54721 tgatactgat cgtcatcaaa cggcatatgc catgcgatgg cggcagcctc ctgcacggtc
    54781 acatcgctga cacgggaaac caactccagc ggcgcgtccg cagacacttt ccctggcgct
    54841 atcacgctat acaaccaccc caccttacct gtgttttgca tcaactgcgc tatatcgctg
    54901 atatcaaaat ggtaattgag tttatagcaa ggcgagcgcg gctggctgac ctgaattaat
    54961 gcctctcccc agcggaaaat atcgcccata tgaacattac tttccgtgag accgtcggtt
    55021 gagagatttt cgccaaacgc aggcgcaaca aacaactccg cctgttccgg aaattcccgc
    55081 acccagtaga gatagtgctc acgaggataa tgacacagcg ctctgtctgg cccgccgtga
    55141 acctttttct ccgcctgctc gtcaccttcc agccccagct ccgtcagcat caactcacca
    55201 tcaacctgga ttttggcaat tgcgctgggt ttgccttcgg gataagcctg aatcttgcct
    55261 gtgtatacat caaccggata tcgcatctgt tccctctcca cgagaaattt ccccattaga
    55321 ccatatttat tttatgtcag ccgtgctgtt ttcgcggtaa gtatccaggc aggaactcaa
    55381 tagcaaaccg gacgaacgtt agttcatccg gctgtgggga
//
LOCUS       DAJTRC010000016        53211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.016, whole genome shotgun sequence.
ACCESSION   DAJTRC010000016 DAJTRC010000000
VERSION     DAJTRC010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 53211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 53211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..53211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.016"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(11..322)
                     /locus_tag="OO357_001045"
     CDS             complement(11..322)
                     /locus_tag="OO357_001045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HCR5886072.1"
                     /translation="MEKEDSFPQRVWQIVAAIPEGYVTTYGDVAKLAGSPRAARQVGG
                     VLKRLPEGSTLPWHRVVNRHGTISLTGPDLQRQRQALLAEGVMVSGSGQIDLQRYRWN
                     Y"
     gene            485..581
                     /gene="ffs"
                     /locus_tag="OO357_001046"
     ncRNA           485..581
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="OO357_001046"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            701..1054
                     /locus_tag="OO357_001047"
     CDS             701..1054
                     /locus_tag="OO357_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1428 domain-containing protein"
                     /protein_id="HCR5886073.1"
                     /translation="MKYVDGFVVAVPADKKDAYREMAAKAAPLFKEFGALRIVECWAS
                     DVPDGKVTDFRMAVKAEENEEVVFSWIEYPSKEVRDAANQKMMSDPRMKEFGESMPFD
                     GKRMIYGGFESIIDE"
     gene            complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OO357_001048"
     CDS             complement(1096..2646)
                     /gene="pdeB"
                     /locus_tag="OO357_001048"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeB"
                     /protein_id="HCR5886074.1"
                     /translation="MRTRHLVGLISGVLILSVLLPVGLSIWLAHQQVETSFIEELDTY
                     SSRVAIRANKVATQGKDALQELERWQGAACSEAHLMEMRRVSYSYRYIQEVVYIDNNV
                     PQCSSLEHESPPDTFPELGKISKDGYRVWLTSHNDLGIIRYMVAMGTAHYVVMIDPAS
                     FIDVIPYSSWQIDAAIIGNAHNVVITSSDEIAQGIITRLQKTPGEHIENNGIIYDILP
                     FLEMNISIITWASTKMLQKGWHRQVFIWLPLGLVIGLLAAMFVLRILRRIQSPHHRLQ
                     DAIENRDICVHYQPIVSLANGKIVGAEALARWPQTDGSWLSPDSFIPLAQQTGLSEPL
                     TLLIIRSVFEDMGDCLRQHPQQHISINLESTVLTSEKIPQLLREMINHYQVNPRQIAL
                     ELTEREFADPKTSAPIISRYREAGHEIYLDDFGTGYSSLSYLQDLDVDILKIDKSFVD
                     ALEYKNVTPHIIEMAKTLKLKMVAEGIETSKQEEWLRQHGVHYGQGWLYSKALPKEDF
                     LRWAEQHL"
     gene            complement(2810..3280)
                     /locus_tag="OO357_001049"
     CDS             complement(2810..3280)
                     /locus_tag="OO357_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaC family protein"
                     /protein_id="HCR5886075.1"
                     /translation="MTEIQRLLTETIESLNTREKRDNKPRFSISFIRKHPGLFIGMYV
                     AFFATLAVMLQSETLSGSVWLLVVLFILLNGFFFFDVYPRYRYEDIDVLDFRVCYNGE
                     WYNTRFVPAALVEAILNSPRVADVHKEQLQKMIVRKGELSFYDIFTLARAESTS"
     gene            complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OO357_001050"
                     /pseudo
     CDS             complement(3396..3947)
                     /gene="maa"
                     /locus_tag="OO357_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414992.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="maltose O-acetyltransferase"
     gene            complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OO357_001051"
     CDS             complement(4119..4337)
                     /gene="hha"
                     /locus_tag="OO357_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5886076.1"
                     /translation="MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFY
                     SAADHRLAELTMNKLYDKIPSSVWKFIR"
     gene            complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OO357_001052"
     CDS             complement(4363..4737)
                     /gene="tomB"
                     /locus_tag="OO357_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hha toxicity modulator TomB"
                     /protein_id="HCR5886077.1"
                     /translation="MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAI
                     NLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYLDDTFMLFSSYGINMQALQKWRK
                     SGNRLFRCFVNATKENPASLSC"
     gene            complement(5283..8432)
                     /locus_tag="OO357_001053"
     CDS             complement(5283..8432)
                     /locus_tag="OO357_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit"
                     /protein_id="HCR5886078.1"
                     /translation="MPNFFIDRPIFAWVIAIIIMLAGGLAILKLPVAQYPTIAPPAVT
                     ISASYPGADAKTVQDTVTQVIEQNMNGIDNLMYMSSNSDSTGTVQITLTFESGTDADI
                     AQVQVQNKLQLAMPLLPQEVQQQGVSVEKSSSSFLMVVGVINTDGTMTQEDISDYVAA
                     NMKDAISRTSGVGDVQLFGSQYAMRIWMNPNELNKFQLTPVDVITAIKAQNAQVAAGQ
                     LGGTPPVKGQQLNASIIAQTRLTSTEEFGKILLKVNQDGSRVLLRDVAKIELGGENYD
                     IIAEFNGQPASGLGIKLATGANALDTAAAIRAELAKMEPFFPSGLKIVYPYDTTPFVK
                     ISIHEVVKTLVEAIILVFLVMYLFLQNFRATLIPTIAVPVVLLGTFAVLAAFGFSINT
                     LTMFGMVLAIGLLVDDAIVVVENVERVMAEEGLPPKEATRKSMGQIQGALVGIAMVLS
                     AVFVPMAFFGGSTGAIYRQFSITIVSAMALSVLVALILTPALCATMLKPIAKGDHGEG
                     KKGFFGWLNRMFEKSTHHYTDSVGGILRSTGRYLVLYLIIVVGMAYLFVRLPSSFLPD
                     EDQGVFMTMVQLPAGATQERTQKVLNEVTHYYLTKEKNNVESVFAVNGFGFAGRGQNT
                     GIAFVSLKDWADRPGEENKVEAITMRATRAFSQIKDAMVFAFNLPAIVELGTATGFDF
                     ELIDQAGLGHEKLTQARNQLLAEAAKHPDMLTSVRPNGLEDTPQFKIDIDQEKAQALG
                     VSINDINTTLGAAWGGSYVNDFIDRGRVKKVYVMSEAKYRMLPDDIGDWYVRAADGQM
                     VPFSAFSSSRWEYGSPRLERYNGLPSMEILGQAAPGKSTGEAMELMEQLASKLPTGVG
                     YDWTGMSYQERLSGNQAPSLYAISLIVVFLCLAALYESWSTPFSVMLVVPLGVIGALL
                     AATFRGLTNDVYFQVGLLTTIGLSAKNAILIVEFAKDLMDKEGKGLIEATLDAVRMRL
                     RPILMTSLAFILGVMPLVISTGAGSGAQNAVGTGVMGGMVTATVLAIFFVPVFFVVVR
                     RRFSRKNEDIEHSHTVDHH"
     gene            complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="OO357_001054"
     CDS             complement(8455..9648)
                     /gene="acrA"
                     /locus_tag="OO357_001054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter periplasmic
                     adaptor subunit AcrA"
                     /protein_id="HCR5886079.1"
                     /translation="MNKNRGFTPLAVVLMLSGSLALTGCDDKQAQQGGQQMPAVGVVT
                     VKTEPLQITTELPGRTSAYRIAEVRPQVSGIILKRNFKEGSDIEAGVSLYQIDPATYQ
                     ATYDSAKGDLAKAQAAANIAQLTVNRYQKLLGTQYISKQGYDQALADAQQANAAVTAA
                     KAAVETARINLAYTKVTSPISGRIGKSNVTEGALVQNGQATVLATVQQLDPIYVDVTQ
                     SSNDFLRLKQELANGTLKQENGKAKVSLITSDGIKFPQDGTLEFSDVTVDQTTGSITL
                     RAIFPNPDHTLLPGMFVRARLEEGLNPNAILVPQQGVTRTPRGDATVLVVGADDKVET
                     RPIVASQAIGDKWLVTEGLKAGDRVVISGLQKVRPGVQVKAQEVTADNNQQAASGAQP
                     EQSKS"
     gene            9790..10437
                     /gene="acrR"
                     /locus_tag="OO357_001055"
     CDS             9790..10437
                     /gene="acrR"
                     /locus_tag="OO357_001055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter transcriptional
                     repressor AcrR"
                     /protein_id="HCR5886080.1"
                     /translation="MARKTKQEAQETRQHILDVALRLFSQQGVSSTSLGEIAKAAGVT
                     RGAIYWHFKDKSDLFSEIWELSESNIGELELEYQAKFPGDPLSVLREILIHVLESTVT
                     EERRRLLMEIIFHKCEFVGEMAVVQQAQRNLCLESYDRIEQTLKHCIEAKMLPADLMT
                     RRAAIIMRGYISGLMENWLFAPQSFDLKKEARDYVAILLEMYLLCPTLRNPATNE"
     gene            10565..13927
                     /gene="mscK"
                     /locus_tag="OO357_001056"
     CDS             10565..13927
                     /gene="mscK"
                     /locus_tag="OO357_001056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel MscK"
                     /protein_id="HCR5886081.1"
                     /translation="MTMFQYYKRSRHFVFSAFIAFVFVLLCQNTAFARASSNGDLPTK
                     ADLQAQLDSLNKQKDLSAQDKLVQQDLTDTLATLDKIDRIKEETVQLRQKVAEAPEKM
                     RQATAALTALSDVDNDEETRKILSTLSLRQLETRVAQALDDLQNAQNDLASYNSQLVS
                     LQTQPERVQNAMYNASQQLQQIRSRLDGTDVGETALRPSQKVLMQAQQALLNAEIDQQ
                     RKSLEGNTVLQDTLQKQRDYVTANSARLEHQLQLLQEAVNSKRLTLTEKTAQEAVSPD
                     EAARIQANPLVKQELEINQQLSQRLITATENGNQLMQQNIKVKNWLERALQSERNIKE
                     QIAVLKGSLLLSRILYQQQQTLPSADELENMTNRIADLRLEQFEVNQQRDALFQSDAF
                     VNKLEEGHTNEVNSEVHDALLQVVDMRRELLDQLNKQLGNQLMMAINLQINQQQLMSV
                     SKNLKSILTQQIFWVNSNRPMDWDWIKAFPQSLKDEFKSMKITVNWEKAWPAVFIAFL
                     AGLPLLLIAGLIHWRLGWLKAYQQKLASAVGSLRNDSQLNTPKAILIDLIRALPVCLI
                     ILAVGLILLTMQLNISELLWSFSKKLAIFWLVFGLCWKVLEKNGVAVRHFGMPEQQTS
                     HWRRQIVRISLALLPIHFWSVVAELSPLHLMDDVLGQAMIFFNLLLIAFLVWPMCRES
                     WRDKESHTMRLVTITVLSIIPIALMVLTATGYFYTTLRLSGRWIETVYLVIIWNLLYQ
                     TVLRGLSVAARRIAWRRALARRQNLVKEGAEGAEPPEEPTIALEQVNQQTLRITMLLM
                     FALFGVMFWAIWSDLITVFSYLDSITLWHYNGTEAGAAVVKNVTMGSLLFAIIASMVA
                     WALIRNLPGLLEVLVLSRLNMRQGASYAITTILNYIIIAVGAMTVFGSLGVSWDKLQW
                     LAAALSVGLGFGLQEIFGNFVSGLIILFERPVRIGDTVTIGSFSGTVSKIRIRATTIT
                     DFDRKEVIIPNKAFVTERLINWSLTDTTTRLVIRLGVAYGSDLEKVRKVLLKAATEHP
                     RVMHEPMPEVFFTAFGASTLDHELRLYVRELRDRSRTVDELNRTIDQLCRENDINIAF
                     NQLEVHLHNEKGDEVTEVKRDYKGDDPTPAVG"
     gene            complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="OO357_001057"
     CDS             complement(14139..14300)
                     /gene="rsmS"
                     /locus_tag="OO357_001057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pleiotropic regulatory protein RsmS"
                     /protein_id="HCR5886082.1"
                     /translation="MSLENAPDDVKLAVDLIVLLEENQIPASTVLRALDIVKRDYEKK
                     LTHDDEAEK"
     gene            complement(14314..14841)
                     /gene="priC"
                     /locus_tag="OO357_001058"
     CDS             complement(14314..14841)
                     /gene="priC"
                     /locus_tag="OO357_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N''"
                     /protein_id="HCR5886083.1"
                     /translation="MKTALLLEKLEGQLATLRQRCAPVSQFATLSARFDRHLFQTRAT
                     TLQSCLDEAGDNLAALRHAVEQQQLPQVAWLAEHLAAQLEAIAREASAWSLREWDSAP
                     PKISRWQRKRIQHQDFERRLREMVAERRARLARVTDLVEQQTLHREVEAYEARLARCR
                     HALEKIENRLARLTR"
     gene            14911..15288
                     /locus_tag="OO357_001059"
     CDS             14911..15288
                     /locus_tag="OO357_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF454 family protein"
                     /protein_id="HCR5886084.1"
                     /translation="MQRIILIIIGWLAVVLGTLGVVLPVLPTTPFILLAAWCFARSSP
                     RFHAWLLYRSWFGSYLRFWQKHHAMPRGVKPRAILLILLTFAISLWFVQMPWVRIMLL
                     VILACLLFYMWRIPVIDEKQEKH"
     gene            15441..15992
                     /gene="apt"
                     /locus_tag="OO357_001060"
     CDS             15441..15992
                     /gene="apt"
                     /locus_tag="OO357_001060"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HCR5886085.1"
                     /translation="MTATAQQLEYLKNSIKSIQDYPKPGILFRDVTSLLEDPKAYALS
                     IDLLVERYKNAGITKVVGTEARGFLFGAPVALGLGVGFVPVRKPGKLPRETISENYDL
                     EYGTDQLEIHVDAIKPGDKVLVVDDLLATGGTIEATVKLIRRLGGEVADAAFIINLFD
                     LGGEQRLEKQGITSYSLVPFPGH"
     gene            16121..18046
                     /gene="dnaX"
                     /locus_tag="OO357_001061"
     CDS             16121..18046
                     /gene="dnaX"
                     /locus_tag="OO357_001061"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HCR5886086.1"
                     /translation="MSYQVLARKWRPQTFADVVGQEHVLTALANGLSLGRIHHAYLFS
                     GTRGVGKTSIARLLAKGLNCETGITATPCGVCDNCREIEQGRFVDLIEIDAASRTKVE
                     DTRDLLDNVQYAPARGRFKVYLIDEVHMLSRHSFNALLKTLEEPPEHVKFLLATTDPQ
                     KLPVTILSRCLQFHLKALDVEQIRHQLEHILNEEHIAHEPRALQLLARAAEGSLRDAL
                     SLTDQAIASGDGQVSTQAVSAMLGTLDDDQALSLVEAMVEGNGERVMALINEAAARGI
                     EWEALLVEMLGLLHRIAMVQLSPAALGNDMAAIELRMRELARTIPPTDIQLYYQTLLI
                     GRKELPYAPDRRMGVEMTLLRALAFHPRMPLPEPEVPRQSFAPVAPTAVMTPTLVPPQ
                     SAPQQAPTVPLPETTSQVLAARQQLQRVQGATKAKKSEPAAATRARPVNNAALERLAS
                     VTDRVQARPVPSALEKAPAKKEAYRWKATTPVMQQKEVVATPKALKKALEHEKTPELA
                     VKLAAEAIERDPWAAQVSQLSLPKLVEQVALNAWKEESDNAVCLHLRSSQRHLNNRGA
                     QQKLAEALSMLKGSTVELTIVEDDNPAVRTPLEWRQAIYEEKLAQARESIIADNNIQT
                     LRRFFDAELDEESIRPI"
     misc_feature    17379..17443
                     /gene="dnaX"
                     /locus_tag="OO357_001061"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00382"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="DnaX frameshifting element; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00382"
     gene            18099..18428
                     /locus_tag="OO357_001062"
     CDS             18099..18428
                     /locus_tag="OO357_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001364632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HCR5886087.1"
                     /translation="MFGKGGLGNLMKQAQQMQEKMQKMQEEIAQLEVTGESGAGLVKV
                     TINGAHNCRRVEIDPSLLEDDKEMLEDLVAAAFNDAARRIEETQKEKMASVSSGMQLP
                     PGFKMPF"
     gene            18428..19033
                     /gene="recR"
                     /locus_tag="OO357_001063"
     CDS             18428..19033
                     /gene="recR"
                     /locus_tag="OO357_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HCR5886088.1"
                     /translation="MQTSPLLTQLMEALRCLPGVGPKSAQRMAFTLLQRDRSGGMRLA
                     QALTRAMSEIGHCADCRTFTEQEVCNICSNPRRQENGQICVVESPADIYAIEQTGQFS
                     GRYFVLMGHLSPLDGIGPDDIGLDRLEQRLAEEKITEVILATNPTVEGEATANYIAEL
                     CAQYDVEASRIAHGVPVGGELEMVDGTTLSHSLAGRHKIRF"
     gene            19143..21017
                     /gene="htpG"
                     /locus_tag="OO357_001064"
     CDS             19143..21017
                     /gene="htpG"
                     /locus_tag="OO357_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003903109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HCR5886089.1"
                     /translation="MKGQETRGFQSEVKQLLHLMIHSLYSNKEIFLRELISNASDAAD
                     KLRFRALSNPDLYEGDGELRVRVSFDKDKRTLTISDNGVGMTRDEVIDHLGTIAKSGT
                     KSFLESLGSDQAKDSQLIGQFGVGFYSAFIVADKVTVRTRAAGEKPENGVFWESAGEG
                     EYTVADITKEDRGTEITLHLREGEDEFLDDWRVRSIISKYSDHIALPVEIEKREEKDG
                     ETVISWAKINKAQALWTRNKSEITDEEYKEFYKHIAHDFNDPLTWSHNRVEGKQEYTS
                     LLYIPSQAPWDMWNRDHKHGLKLYVQRVFIMDDAEQFMPNYLRFVRGLIDSSDLPLNV
                     SREILQDSTVTRNLRNALTKRVLQMLEKLAKDDAEKYQTFWQQFGLVLKEGPAEDFAN
                     QEAIAKLLRFASTYTDSSAQTVSLEDYVSRMKEGQEKIYYITADSYAAAKSSPHLELL
                     RKKGIEVLLLSDRIDEWMMNYLTEFDGKPFQSVSKVDESLEKLADEVDESAKEAEKAL
                     TPFIDRVKALLGERVKDVRLTHRLTDTPAIVSTDADEMSTQMAKLFAAAGQKVPEVKY
                     IFELNPDHVLVKRAADTEDEAKFSEWVELLLDQALLAERGTLEDPNLFIRRMNQLLVS
                     "
     gene            21198..21842
                     /gene="adk"
                     /locus_tag="OO357_001065"
     CDS             21198..21842
                     /gene="adk"
                     /locus_tag="OO357_001065"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007706188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HCR5886090.1"
                     /translation="MRIILLGAPGAGKGTQAQFIMEKYGIPQISTGDMLRAAVKSGSE
                     LGKQAKDIMDAGKLVTDELVIALVKERIAQEDCRNGFLLDGFPRTIPQADAMKEAGIN
                     VDYVLEFDVPDELIVDRIVGRRVHAPSGRVYHVKFNPPKVEGKDDVTGEELTTRKDDQ
                     EETVRKRLVEYHQMTAPLIGYYSKEAEAGNTKYAKVDGTKQVAEVRADLEKILG"
     gene            21974..22936
                     /gene="hemH"
                     /locus_tag="OO357_001066"
     CDS             21974..22936
                     /gene="hemH"
                     /locus_tag="OO357_001066"
                     /EC_number="4.98.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HCR5886091.1"
                     /translation="MRQTKTGILLANLGTPDAPTPEAVKRYLKQFLSDRRVVDTSRLL
                     WWPLLRGVILPLRSPRVAKLYASVWMEDGSPLMVYSRQQQQALAQRLPDTPVALGMSY
                     GSPSLESSVDELLAEHVDHIVVLPLYPQFSCSTVGAVWDELARILARKRSIPGISFIR
                     DYADNHDYINALANSVRASFAKHGEPDLLLLSYHGIPQRYADEGDDYPQRCRTTTREL
                     ASALGMVPEKVMMTFQSRFGREPWLMPYTDETLKMLGEKGVGHIQVMCPGFAADCLET
                     LEEIAEQNREVFLGAGGKKYEYIPALNATPEHIEMMANLVAAYR"
     gene            complement(22933..23892)
                     /gene="aes"
                     /locus_tag="OO357_001067"
                     /pseudo
     CDS             complement(22933..23892)
                     /gene="aes"
                     /locus_tag="OO357_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415009.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl esterase"
     gene            24044..25348
                     /gene="gsk"
                     /locus_tag="OO357_001068"
     CDS             24044..25348
                     /gene="gsk"
                     /locus_tag="OO357_001068"
                     /EC_number="2.7.1.73"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/guanosine kinase"
                     /protein_id="HCR5886092.1"
                     /translation="MKFPGKRKSKHYFPVNARDPLLQQFQPENETSAAWVVGIDQTLV
                     DIEAKVDDEFIERYGLSAGHSLVIEDDVAEVLYQELKQKNLITHQFAGGTIGNTMHNY
                     SVLADDRSVLLGVMCSNIEIGSYAYRYLCNTSSRTDLNYLQGVDGPIGRCFTLIGESG
                     ERTFAISPGHMNQLRAESIPEDVIAGASALVLTSYLVRCKPGEPMPEATMKAIEYAKK
                     YNVPVVLTLGTKFVIAENPQWWQQFLKDHVSILAMNEDEAEALTGESDPLLASDKALD
                     WVELVLCTAGPIGLYMAGFTEDEAKRKTQHPLLPGAIAEFNQYEFSRAMRHKDCQNPL
                     RVYSHIAPYMGGPEKIMNTNGAGDGALAALLHDITANSYHRSNVPNSSKHKFTWLTYS
                     SLAQVCKYANRVSYQVLNQHSPRLTRGLPEREDSQEESYWDR"
     gene            complement(25481..27157)
                     /locus_tag="OO357_001069"
     CDS             complement(25481..27157)
                     /locus_tag="OO357_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Kef family K(+) transporter"
                     /protein_id="HCR5886093.1"
                     /translation="MHHATPLITTIVGGLVLAFILGMLANKLRISPLVGYLLAGVLAG
                     PFTPGFVADTKLAPELAELGVILLMFGVGLHFSLKDLMAVKSIAIPGAIAQIAVATLL
                     GMALSAVLGWSLMTGIVFGLCLSTASTVVLLRALEERQLIDSQRGQIAIGWLIVEDLV
                     MVLTLVLLPAVAGMMEQGDVGFATLAVDMGITIGKVIAFIAIMMLVGRRLVPWIMARS
                     AATGSRELFTLSVLALALGIAFGAVELFDVSFALGAFFAGMVLNESELSHRAAHDTLP
                     LRDAFAVLFFVSVGMLFDPLILIQQPLAVLATLAIILFGKSVAALFLVRLFGHSQRTA
                     LTIAASLAQIGEFAFILAGLGMALNLLPQAGQNLVLAGAILSIMLNPVLFALLEKYLA
                     KTETLEEQTLEEAIEEEKQIPVDICNHALLVGYGRVGSLLGEKLLASDIPLVVIETSR
                     TRVDELRERGVRAVLGNAANEEIMQLAHLECAKWLILTIPNGYEAGEIVASARAKNPD
                     IEIIARAHYDDEVTYITERGANQVVMGEREIARTMLELLETPPAGEVVTG"
     gene            complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="OO357_001070"
     CDS             complement(27395..28615)
                     /gene="fsr"
                     /locus_tag="OO357_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fosmidomycin MFS transporter"
                     /protein_id="HCR5886094.1"
                     /translation="MAMSEQTQPVAGAAASTTKARTSFGILGAISLSHLLNDMIQSLI
                     LAIYPLLQSEFSLTFMQIGMITLTFQLASSLLQPVVGYWTDKYPMPWSLPIGMCFTLS
                     GLVLLALAGSFGAVLLAAALVGTGSSVFHPESSRVARMASGGRHGLAQSIFQVGGNFG
                     SSLGPLLAAVIIEPYGKGNVAWFVLAALLAIVVLAQISRWYSAQHRMNKGKPKATIIN
                     PLPRNKVVLAVSILLILIFSKYFYMASISSYYTFYLMQKFGLSIQNAQLHLFAFLFAV
                     AAGTVIGGPVGDKIGRKYVIWGSILGVAPFTLILPYARLHWTGVLTVIIGFILASAFS
                     AILVYAQELLPGRIGMVSGLFFGFAFGMGGLGAAVLGLIADHTSIELVYKICAFLPLL
                     GMLTIFLPDNRHKD"
     gene            28833..30485
                     /gene="ushA"
                     /locus_tag="OO357_001071"
     CDS             28833..30485
                     /gene="ushA"
                     /locus_tag="OO357_001071"
                     /EC_number="3.1.3.5"
                     /EC_number="3.6.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5886095.1"
                     /translation="MKLLQRGVALALLTTFTLASETALAYEQDKTYKITVLHTNDHHG
                     HFWRNEYGEYGLAAQKTLVDGIRKEVAAEGGSVLLLSGGDINTGVPESDLQDAEPDFR
                     GMNLVGYDAMAIGNHEFDNPLTVLRQQEKWAKFPLLSANIYQKSTGERLFKPWALFKR
                     QDLKIAVIGLTTDDTAKIGNLEYFTDIEFRKPADEAKLVIQELQQTEKPDIIIAATHM
                     GHYDNGEHGSNAPGDVEMARALPAGSLAMIVGGHSQDPVCMAAENKKQVDYVPGTPCK
                     PDQQNGIWIVQAHEWGKYVGRADFEFRNGEMKMVNYQLIPVNLKKKVTWEDGKSERVL
                     YTPEIAENQQMISLLSPFQNKGKAQLEVKIGKTNGRLEGDRDKVRFVQTNMGRLILAA
                     QMDRTGADFAVMSGGGIRDSIEAGDISYKNVLKVQPFGNVVVYADMTGKEVIDYLTAV
                     AQMKPDSGAYPQFANVSFVAKDGKLNDLKIKGEPVDPAKTYRMATLNFNATGGDGYPR
                     LDNKPGYVNTGFIDAEVLKAYIQKSSPLDVSVYEPKGEVSWQ"
     gene            complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="OO357_001072"
     CDS             complement(30522..31001)
                     /gene="ybaK"
                     /locus_tag="OO357_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK"
                     /protein_id="HCR5886096.1"
                     /translation="MTPAVKLLEKNKISFQIHTYEHDPAETNFGDEVVKKLGLNPDQV
                     YKTLLVAVNGDMKHLAVAVTPVAGQLDLKKVAKALGAKKVEMADPMVAQRSTGYLVGG
                     ISPLGQKKRLPTIIDAPAQEFATIYVSGGKRGLDIELAAGDLAKILDAKFADIARRD"
     gene            complement(31205..31999)
                     /locus_tag="OO357_001073"
     CDS             complement(31205..31999)
                     /locus_tag="OO357_001073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HCR5886097.1"
                     /translation="MDLLYRVKTLWAALRGNHYTWPAIDITLPGNRHFHLIGSIHMGS
                     HDMAPLPTRLLKKLKNADALIVEADVSTSDTPFANLPACEALEERISEEQLQNLQHIS
                     QEMGISPSLFSTQPLWQIAMVLQATQAQKLGLRAEYGIDYQLLQAAKQQHKPVIELEG
                     AENQIAMLLQLPDKGLALLDDTLTHWHTNARLLQQMMSWWLNAPPQNNDITLPNTFSQ
                     SLYDVLMHQRNLAWRDKLRAMPPGRYVVAVGALHLYGEGNLPQMLR"
     gene            32137..32478
                     /locus_tag="OO357_001074"
     CDS             32137..32478
                     /locus_tag="OO357_001074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5886098.1"
                     /translation="MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNRVSAL
                     INNNRKLTTEMAFRLAKVFDTTVDFRLNLQAAVDLWEVENNMRTQEELGRIETVAEYL
                     ARREERAKKVA"
     gene            complement(32693..35197)
                     /gene="copA"
                     /locus_tag="OO357_001075"
     CDS             complement(32693..35197)
                     /gene="copA"
                     /locus_tag="OO357_001075"
                     /EC_number="7.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-exporting P-type ATPase CopA"
                     /protein_id="HCR5886099.1"
                     /translation="MSQTIDLNLDGLSCGHCVKRVKESLEQRPDVEQADVSITEAHVT
                     GTASAEQLIETIKQAGYDASVSHPKAKPLAESSIPSEALTAVSEALPAATADDDDSQQ
                     LLLSGMSCASCVTRVQNALQSVPGVTQARVNLAERTALVMGSASPQDLVQAVEKAGYG
                     AEAIEDDAKRRERQQETAVATMKRFRWQAIVALAVGIPVMVWGMIGDNMIVTADNRSL
                     WLVIGLITLAVMVFASGHFYRSAWKSLLNGAATMDTLVALGTGVAWLYSMSVNLWPQW
                     FPMEARHLYYEASAMIIGLINLGHMLEARARQRSSKALEKLLDLTPPTARLVTDEGEK
                     SVPLAEVQPGMLLRLTTGDRVPVDGEITQGEAWLDEAMLTGEPIPQQKGEGDSVHAGT
                     VVQDGSVLFRASAVGSHTTLSRIIRMVRQAQSSKPEIGQLADKISAIFVPVVVVIALV
                     SAAIWYFFGPAPQIVYTLVIATTVLIIACPCALGLATPMSIISGVGRAAEFGVLVRDA
                     DALQRASTLDTVVFDKTGTLTEGKPQVVAVKTFADVDEAQALRLAAALEQGSSHPLAR
                     AILDKAGDMQLPQVNGFRTLRGLGVSGEAEGHALLLGNQALLNDQQVDTKAIEADISA
                     QASQGATPVLLAIDGKAVALLAVRDPLRSDSVAALQRLHKAGYRLVMLTGDNPTTANA
                     IAKEAGIDEVIAGVLPDGKAEAIKRLQSEGRQVAMVGDGINDAPALAQADVGIAMGGG
                     SDVAIETAAITLMRHSLMGVADALAISRATLRNMKQNLLGAFIYNSIGIPVAAGILWP
                     FTGTLLNPVVAGAAMALSSITVVSNANRLLRFKPKE"
     gene            35459..36391
                     /gene="glsA"
                     /locus_tag="OO357_001076"
     CDS             35459..36391
                     /gene="glsA"
                     /locus_tag="OO357_001076"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HCR5886100.1"
                     /translation="MLDANKLQQAVDQAYTQFHSLNGGQNADYIPFLANVPGQLAAVA
                     IVTCDGNVYSAGDSDYRFALESISKVCTLALALEDVGPQAVQDKIGADPTGLPFNSVI
                     ALELHGGKPLSPLVNAGAIATTSLINAENAEQRWQRILHIQQQLAGEQVALSDEVNQS
                     EQTTNFHNRAIAWLLYSAGYLYCDAMEACDVYTRQCSTLINTVELATLGATLAAGGVN
                     PLTHKRVLQADNVPYILAEMMMEGLYGRSGDWAYRVGLPGKSGVGGGILAVVPGVMGI
                     AAFSPPLDEEGNSVRGQKMVASVAKQLGYNVFKG"
     gene            36394..37686
                     /locus_tag="OO357_001077"
     CDS             36394..37686
                     /locus_tag="OO357_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5886101.1"
                     /translation="MMNTEGNNGNKPLGLWNVVSIGIGAMVGAGIFALLGQAALLMEA
                     STWGAFAFGGIVAMFSGYAYARLGASYPSNGGIIDFFRRGLGNGVFSLALSLLYLLTL
                     AVSIAMVARAFGAYAVQFLHEGSQEEHLILLYALGIIAVMTLFNSLSNHAVGRLEVIL
                     VGIKMMILLLLIIAGVWSLQPAHISVSAPPSSGAFFSCIGITFLAYAGFGMMANAADK
                     VKDPQVIMPRAFLVAIGVTTLLYISLALVLLSDVSALELEKYADTAVAQAASPLLGHV
                     GYVIVVIGALLATASGINANLFAVFNIMDNMGSERELPKLMNKSLWRQSTWGNIIVVV
                     LIMLMTAALNLDSLASVASATFLICYLAVFVVAIRLRHDIHASLPILIVGTLVMLLVI
                     VGFIYSLWSQGSRALIWIIGAILLSLIVAMVMKRNKTV"
     gene            37811..38218
                     /gene="cueR"
                     /locus_tag="OO357_001078"
     CDS             37811..38218
                     /gene="cueR"
                     /locus_tag="OO357_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HCR5886102.1"
                     /translation="MNISDVAKITGLTSKAIRFYEEKGLVTPPMRSENGYRTYTQQHL
                     NELTLLRQARQVGFNLEESGELVNLFNDPQRHSADVKRRTLEKVAEIERHIEELQSMR
                     DQLLALANACPGDDSADCPIIENLSGCCHHRAG"
     gene            complement(38219..38677)
                     /locus_tag="OO357_001079"
     CDS             complement(38219..38677)
                     /locus_tag="OO357_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415021.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HCR5886103.1"
                     /translation="MMELMVVHPHIFWLSLGGLLLAAEMLGGNGYLLWSGVAAVITGL
                     VVWLVPLGWEWQGVMFAILTLLAAWLWWKWLSRRVREQKHSDSHLNQRGQQLIGRRFV
                     LESPLVNGRGHMRVGDSSWPVSASEDLGAGTHVEVIAIEGITLHIRAVSS"
     gene            complement(38674..39591)
                     /locus_tag="OO357_001080"
     CDS             complement(38674..39591)
                     /locus_tag="OO357_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5886104.1"
                     /translation="MLIFIPILIFVALVIVGAGVKIVPQGYQWTVERFGRYTKTLQPG
                     LSLVVPFMDRIGRKINMMEQVLDIPSQEVISKDNANVTIDAVCFIQVIDAPRAAYEVS
                     NLELAIINLTMTNIRTVLGSMELDEMLSQRDSINSRLLRIVDEATNPWGIKVTRIEIR
                     DVRPPAELISSMNAQMKAERTKRAYILEAEGIRQAEILKAEGEKQSQILKAEGERQSA
                     FLQAEARERSAEAEARATKMVSEAIASGDIQAVNYFVAQKYTEALQQIGSSSNSKVVM
                     MPLEASSLMGSIAGIAELVKDSANKRTQP"
     gene            39737..40414
                     /gene="fetA"
                     /locus_tag="OO357_001081"
     CDS             39737..40414
                     /gene="fetA"
                     /locus_tag="OO357_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter ATP-binding protein FetA"
                     /protein_id="HCR5886105.1"
                     /translation="MQENSPLLQLQNVGYLAGDTKILNNINFSLRAGEFKLITGPSGC
                     GKSTLLKIVASLISPTSGTLLFEGEDVSTLKPEIYRQQVSYCAQTPTLFGDTVYDNLI
                     FPWQIRDQQPDPAIFLDFLERFALPDSILTKNIAELSGGEKQRISLIRNLQFMPKVLL
                     LDEITSALDESNKHNVNEMIHRYVREQNIAVLWVTHDKDEINHADKVITLQPHAGEMQ
                     EARYELA"
     gene            40401..41180
                     /gene="fetB"
                     /locus_tag="OO357_001082"
     CDS             40401..41180
                     /gene="fetB"
                     /locus_tag="OO357_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron export ABC transporter permease subunit
                     FetB"
                     /protein_id="HCR5886106.1"
                     /translation="MNSHNITNESLALALMLVVVAILISHKEKLALEKDILWSVGRAI
                     IQLIIVGYVLKYIFSVDDASLTLLMVLFICFNAAWNAQKRSKYIAKAFISSFIAITVG
                     AGITLAVLILSGAIEFIPMQVIPVAGMIAGNAMVAVGLCYNNLGQRVISEQQQIQEKL
                     SLGATPKQASAILIRDSIRAALIPTVDSAKTVGLVSLPGMMSGLIFAGIDPVKAIKYQ
                     IMVTFMLLSTASLSTIIACYLTYRKFYNSRHQLVVTQLKKK"
     gene            complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="OO357_001083"
     CDS             complement(41243..42097)
                     /gene="cnoX"
                     /locus_tag="OO357_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperedoxin"
                     /protein_id="HCR5886107.1"
                     /translation="MSVENIVNINESNLQQVLEQSMTTPVLFYFWSERSQHCLQLTPI
                     LESLAAQYNGQFILAKLDCDAEQMIAAQFGLRAIPTVYLFQNGQPVDGFQGPQPEEAI
                     RALLDKVLPREEELKAQQAMQLMQEGNYTDALPLLKDAWQLSNQNGEIGLLLAETLIA
                     LNRSEDAEAVLKTIPLQDQDTRYQGLVAQIELLKQAADKPEIQQLQQQVAENPEDAAL
                     ATQLALQLHQVGRNEEALELLFGHLRKDLTAADGQTRKTFQEILAALGTGDALASKYR
                     RQLYALLY"
     gene            complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="OO357_001084"
     CDS             complement(42158..42967)
                     /gene="ybbO"
                     /locus_tag="OO357_001084"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(+)-dependent aldehyde reductase"
                     /protein_id="HCR5886108.1"
                     /translation="MTHKATEILTGKVMQKSVLITGCSSGIGLESALELKRQGFHVLA
                     GCRKPNDVERMNNMGFTGVLIDLDSPESVNRAADEVIALTDNCLYGIFNNAGFGMYGP
                     LSTISRAQMEQQFSANFFGAHQLTMRLLPAMLPHGEGRIVMTSSVMGLISTPGRGAYA
                     ASKYALEAWSDALRMELRHSGIKVSLIEPGPIRTRFTDNVNQTQSDKPVENPGIAARF
                     TLGPEAVVDKVRHAFISEKPKMRYPVTLVTWAVMVLERLLPGRVMDKILQG"
     gene            complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="OO357_001085"
                     /pseudo
     CDS             complement(42957..43579)
                     /gene="tesA"
                     /locus_tag="OO357_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005225520.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional acyl-CoA thioesterase I/protease
                     I/lysophospholipase L1"
     gene            43550..44236
                     /locus_tag="OO357_001086"
     CDS             43550..44236
                     /locus_tag="OO357_001086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5886109.1"
                     /translation="MPAENIVEVHHLKKSVGQGEHELSILTGVELVVKRGETIALVGE
                     SGSGKSTLLAILAGLDDGSSGEVSLVGQPLHNMDEEARAKLRAKHVGFVFQSFMLIPT
                     LNALENVELPALLRGESSAESRNGAKALLEQLGLGKRLDHLPAQLSGGEQQRVALARA
                     FNGRPDVLFADEPTGNLDRQTGDKIADLLFSLNREHGTTLIMVTHDLQLAARCDRCLR
                     LVNGQLQEEA"
     gene            44233..46648
                     /locus_tag="OO357_001087"
                     /pseudo
     CDS             44233..46648
                     /locus_tag="OO357_001087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="OO357_001088"
     CDS             complement(47361..48455)
                     /gene="mnmH"
                     /locus_tag="OO357_001088"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-selenouridine(34) synthase MnmH"
                     /protein_id="HCR5886110.1"
                     /translation="MQERHTEQDYRALLIADTPIIDVRAPIEFEQGAMPAAINLPLMD
                     NDERASVGTCYKQQGSDAALALGHKLVAGEIRQQRMYAWRAACLQNPQGILCCARGGQ
                     RSHIVQRWLHEAGIDYPLVEGGYKALRQTAIQATIELAQKPIVLIGGCTGSGKTLLVQ
                     QQPNGVDLEGLARHRGSAFGRTLQPQLSQASFENLLAAEMLKTDARQDLRLWVLEDES
                     RMIGSNHLPECLRERMTQAAIAVVEDPFEIRLERLNEEYFLRMHHDFTHAYGDEQGWQ
                     EYCEYLHHGLSAIKRRLGLQRYNELAAQLDTALTTQLTTGSTDGHLAWLVPLLKEYYD
                     PMYRYQLEKKAEKVVFRGEWAEVAVWVKAQ"
     gene            complement(48524..49450)
                     /gene="allS"
                     /locus_tag="OO357_001089"
     CDS             complement(48524..49450)
                     /gene="allS"
                     /locus_tag="OO357_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AllS"
                     /protein_id="HCR5886111.1"
                     /translation="MFDPETLRTFIAVAETGSFSKAAERLCKTTATISYRIKLLEENT
                     GVALFFRTTRSVTLTAAGEHLLSQARDWLSWLESMPSELQQVNDGVERQVNIVINNLL
                     YNPQAVAQLLAWLNERYPFTQFHISRQIYMGVWDSLLYEGFSLAIGVTGTEALANTFS
                     LDPLGSVQWRFVMAADHPLANVEEPLTEAQLRRFPAVNIEDSAGTLTKRVAWRLPGQK
                     EIIVPDMETKIAAHLAGVGIGFLPKSLCQSMIDNQQLVSRVIPTMRPPSPLSLAWRKF
                     GSCKAVEDIVTLFTQHRPEISGFLEIFGTPRS"
     gene            49680..50162
                     /gene="allA"
                     /locus_tag="OO357_001090"
     CDS             49680..50162
                     /gene="allA"
                     /locus_tag="OO357_001090"
                     /EC_number="4.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ureidoglycolate lyase"
                     /protein_id="HCR5886112.1"
                     /translation="MKLQVLPLSQEAFSAYGDVIETQQRDFFHINNGLVERYHDLALV
                     EILEQDRTLISINRAQPANLPLTIHELERHPLGIQAFIPMKGEVFVVVVALGDDKPDL
                     STLRAFITNGEQGVNYHRNVWHHPLFAWQRVTDFLTIDRGGSDNCDVESIPEQELCFA
                     "
     gene            50240..51055
                     /gene="allR"
                     /locus_tag="OO357_001091"
     CDS             50240..51055
                     /gene="allR"
                     /locus_tag="OO357_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor AllR"
                     /protein_id="HCR5886113.1"
                     /translation="MTEVRRRGRPGQAEPVAQKGAQALERGIAILQYLEKSGGSSSVS
                     DISLNLDLPLSTTFRLLKVLQAADFVYQDSQLGWWHIGLGVFNVGAAYIHNRDVLSVA
                     GPFMRRLMLLSGETVNVAIRNGNEAVLIGQLECKSMVRMCAPLGSRLPLHASGAGKAL
                     LYPLAEEELMSIILQTGLQQFTPTTLVDMPTLLKDLEQARELGYTVDKEEHVVGLNCI
                     ASAIYDDVGRVVAAISISGPSSRLTEDRFVSQGELVRDTARDISTALGLKAHP"
     gene            51145..52926
                     /gene="gcl"
                     /locus_tag="OO357_001092"
     CDS             51145..52926
                     /gene="gcl"
                     /locus_tag="OO357_001092"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HCR5886114.1"
                     /translation="MAKMRAVDAAMYVLEKEGITTAFGVPGAAINPFYSAMRKHGGIR
                     HILARHVEGVSHMAEGYTRATAGNIGVCLGTSGPAGTDMITALYSASADSIPILCITG
                     QAPRARLHKEDFQAVDIEAIAKPVSKMAVTVREAALVPRVLQQAFHLMRSGRPGPVLV
                     DLPFDVQVAEIEFDPDMYEPLPVYKPAASRMQIEKAVEMLIQAERPVIVAGGGVINAD
                     AAALLQQFAELTSVPVIPTLMGWGCIPDDHELMAGMVGLQTAHRYGNATLLASDMVFG
                     IGNRFANRHTGSVEKYTEGRKIVHIDIEPTQIGRVLCPDLGIVSDAKAALTLLVEVAQ
                     EMQKAGRLPCRKEWVADCQQRKRTLLRKTHFDNVPVKPQRVYEEMNKAFGRDVCYVTT
                     IGLSQIAAAQMLHVFKDRHWINCGQAGPLGWTIPAALGGCAADPKRNVVAISGDFDFQ
                     FLIEELAVGAQFNIPYIHVLVNNAYLGLIRQSQRAFDMDYCVQLAFENINSSEVNGYG
                     VDHVKVAEGLGCKAIRVFKPEDIAPAFEQAKALMAQYRVPVVVEVILERVTNISMGSE
                     LDNVMEFEDIADNAADAPTETCFMHYE"
     gene            52939..>53211
                     /locus_tag="OO357_001093"
     CDS             52939..>53211
                     /locus_tag="OO357_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HCR5886115.1"
                     /translation="MLRFSANLSMLFGEYDFLARFEKAAQCGFRGVEFMFPYDYDIEE
                     LKQVLASNKLEHTLHNLPAGDWAAGERGIACIPGREEEFRDGVAAAI"
BASE COUNT        12830 a        13690 c        14016 g        12675 t
ORIGIN      
        1 ccggagaggg ttagtagttc cagcgataac gctgcaagtc gatttgcccg cttcccgata
       61 ccatcacacc ttctgccagt aatgcctgtc gctgacgctg taaatccggc ccggttagcg
      121 aaattgtgcc atggcgatta accacccggt gccagggtaa ggtgctgcct tcagggagac
      181 gctttaacac accgcccacc tggcgcgcgg cgcggggcga tcccgccagt ttcgccacat
      241 caccgtaagt ggtgacatag ccttcgggaa tagcggcgac gatttgccag acgcgttggg
      301 gaaatgaatc ttctttttcc atcttttctt cctgaggtaa tttttcagca taatctggaa
      361 aaacgcccga gtgaagtcgc attgcgcaag aaaccagcat ctggcacgca atgggttgca
      421 attagccggg gcagcagtga taatgcgcct gcgcgttggt tttcaacgct ctcaatgggg
      481 gctctgttgg ttctcccgca atgctactct gtttaccagg tcaggtccgg aaggaagcag
      541 ccaaggcaga tgacgcgtgt gccgggatgt agctggcagg gcccccaccc atttctgcct
      601 cccaccgttt cgtcaaaaaa tcccagcatg gctaaacttt aaccacaact gacgtcgcaa
      661 gaattgtctg gctgcgcagt acgcttcgga ggtatgtctg atgaagtatg ttgatggttt
      721 tgtggttgcc gttcctgccg ataaaaagga tgcctatcgg gaaatggccg ctaaggccgc
      781 gccattgttt aaagagtttg gcgcgcttcg tattgtcgaa tgctgggcca gcgatgtgcc
      841 ggatggcaaa gtgaccgatt ttcgtatggc ggtgaaagcg gaagagaatg aagaggttgt
      901 ctttagctgg attgaatacc cttcaaaaga ggtccgcgac gcagctaatc aaaagatgat
      961 gtcggaccca cggatgaaag agttcggcga gtccatgccg tttgacggca agcgaatgat
     1021 ctatggcgga ttcgagtcaa tcatcgacga ataggtagcg tgacgggctg tgcggcgtgc
     1081 cgtcagccca aaaattcaca aatgttgctc ggcccagcgt aagaaatctt ctttcggtaa
     1141 tgccttgctg tagagccagc cctgaccgta gtgcacgcca tgctggcgta gccactcttc
     1201 ttgtttactg gtttcgattc cctccgctac cattttcagt ttcagtgttt ttgccatttc
     1261 gatgatatgc ggcgtgacat ttttatattc cagcgcatca acgaaagatt tatcgatctt
     1321 cagaatgtcg acatccagat cctgtaaata acttaaactt gaataccccg taccaaaatc
     1381 atcaagataa atttcatggc ccgcctcccg gtagcgagaa attatcgggg cgctggtttt
     1441 cggatcggca aactcgcgtt cagtgagttc aagcgcgatc tgtctgggat taacctgata
     1501 gtgattgatc atttcacgca gcaattgcgg gattttttcc gaggtgagca cggtggattc
     1561 aagattgatc gaaatatgct gctgtggatg ctgacgcaag cagtcgccca tatcttcaaa
     1621 gacgcttctt ataatcagta gcgtcaatgg ctcagaaagg cccgtttgct gtgccagcgg
     1681 aataaaacta tctggtgaca accaactacc gtctgtctgc ggccagcgcg ccagtgcctc
     1741 agcaccgaca attttgccat tggctaagga gacaatcggc tgatagtgca cgcaaatatc
     1801 acgattttcg atagcatcct gcagccgatg atgcggtgat tggatacggc gcaaaatacg
     1861 cagcacaaac atcgctgcca gcaggccaat caccaacccg agcggtaacc aaataaagac
     1921 ttgccgatgc cagcctttct gcaacatttt cgttgaagcc caggtgatga tcgaaatatt
     1981 catctccagg aagggcagta tatcgtaaat gatcccatta ttttcgatat gctcaccggg
     2041 tgttttttgt agcctggtaa taattccctg agcaatttca tcgctgctgg ttatgacaac
     2101 gttatgggca ttgccaataa tggcggcatc aatttgccat gaactatagg gaatgacatc
     2161 aataaaggaa gcggggtcga tcatgacgac ataatgtgcc gttcccatgg cgaccatgta
     2221 acggataatg cctaaatcgt tatgcgatgt taaccagaca cgataaccat ctttcgaaat
     2281 tttacctagc tcgggaaagg tatcgggcgg actttcatgc tccagagacg aacactgggg
     2341 aacgttgtta tcgatataaa ccacttcctg aatatagcga taactgtaag atacccgacg
     2401 catttccatg agatgggctt cgctacaggc agcgccttgc catctttcca gctcctgcag
     2461 cgcatctttc ccttgtgtcg ccaccttatt ggctcgaata gcgacgcggg aggaataggt
     2521 atccagctct tcaataaacg atgtttctac ctgctgatgg gccagccaga tgcttaagcc
     2581 gacaggcagc aatactgaaa gaatcagtac tcccgaaata aggccgacca gatgtcgtgt
     2641 tctcaccagc atatccttat taacacagtg tagggaaagt atatctgatt gtggaaaaag
     2701 gctggcagga gataaggagg tagagctggt atttcatgaa gtgatgggtg gaaacgatca
     2761 ttgtcatttg accaggcgca gagctttacg cctggtatgt aaccctaact taagatgttg
     2821 attcggcgcg agcgagggta aaaatatcgt aaaaagacag ttcaccttta cggacgatca
     2881 ttttttgcag ttgttcctta tgaacatccg cgacacgcgg agagttcaag atggcttcaa
     2941 ccagcgcggc aggtacaaag cgcgtgttgt accattcgcc gttatagcaa acgcggaaat
     3001 ccagcacgtc gatatcttca tagcggtagc gtgggtagac atcgaaaaag aagaaaccat
     3061 taagcaggat aaataataca accagtagcc agacagagcc tgacagcgtt tcggactgca
     3121 acatcaccgc cagggtggca aaaaaagcaa cgtacatacc gataaacagc cccggatgtt
     3181 tacggataaa actgatacta aagcggggtt tgttgtcgcg tttttcgcgg gtattcagag
     3241 actcaatcgt ttcggtcagc aggcgttgta tttcggtcat cgtttgcctt cgtggtatct
     3301 gcaactttcc agggaacacc ctattttaac gtgggaatag tagaggggaa gtaacagatc
     3361 taccacaatt ttgcataaca gttgcgaaaa accgattaca attttttaat tattctggct
     3421 ggattaccgc ccacgacaac gttgtccggg acaccttttg tgacaactgc acctgaagct
     3481 accacgacgt tatcaccaat ggtcacacca gggttaatga ccgcgcgtcc gccaatccag
     3541 acgttattac cgatggtgac gggtttcccc agttcagcac cgctattacg tgctacaggg
     3601 tcgatgggat gtgttgccgt gtagatatga acgcctggtg ccaacataca gttatcaccg
     3661 atgcgaatag ggcagacatc aagcatcacg caatcgaagt tggcgaaaaa attattgccg
     3721 agaaaaatgt tatagccata atcacagcga aacgttggct caatataagc ctctgtcacc
     3781 tgaccgaata aattagcgag aatttactgg cgtaatgtgt gctcttccgc cagggaatga
     3841 ttgtatcggt gaataagctg acgagcgcgc aggcgatcgc gagataacgt ctcatctgcc
     3901 gagcgataca actcaccagc aatcatcttt tctttttctg tgctcattac tgacctccgg
     3961 ttgctgaaaa tgcggcaaca cagtaagacg aaaagtcagt gaagagaacg ttctcattac
     4021 atctgtgtga taaagatcac atagggtatg tcttcatggc gaaaaagtat aaaattctta
     4081 ataaacagcc ggttatagca ccgaaagcga attatttatt agcgaataaa tttccatact
     4141 gaggaaggga tcttgtcgta cagtttattc atggtcaatt cggcgaggcg gtgatctgcg
     4201 gctgagtaaa ataccgccag ttcattatct gataattcgt atttattttt ctcgataaca
     4261 cgctccagcg tgtcaattgt ctggcaacga cgtaaacgca ttaaataatc ggtttttgtt
     4321 aaaggttttt cggacatact tctacccatg gttgtaataa ttctaacaag ataaactcgc
     4381 aggattctct ttcgtcgcgt tgacaaaaca acggaatagt cgattacctg acttccgcca
     4441 tttctgaaga gcctgcatat taataccata actgctgaac aacataaagg tgtcatccag
     4501 atattcgtcg atctgctcaa tgagcttatt gtcttcatta tacttaattt tgtaattaag
     4561 tgcgaaggtc gcaatatgct caatcagttc attcaactgg aggttgatcg ccgaggttgg
     4621 gtcgttaacc caaccatgat tgctttcttc aaggtttgca agacagtcat gatacagggt
     4681 ttcacagaga aacttaagct gtgcgatatc atgtcttttg ggtgagtatt catccataac
     4741 gcgtcccctt cttagtggtt gaactaacgg acacctttcg ggatggaaaa aacttactga
     4801 cctagacttg ccctctcgtt tgttagctta actataagcc actctttgca ggttttcatc
     4861 gcattattac gaaacattac aattaggaag gaatcgggac aggactcact ttttcatact
     4921 agcactaacc cgcagcaggt aaaagcagtt tgctcgaact gttcataaca tttcaggatt
     4981 atcgccagat gaaattggtg acactaatac caggattgct ctgaatatga cgtaataacc
     5041 gaggaatgaa taaagaatta ccgcaaataa ttaagaatag cctctaaatg attatggatt
     5101 attaaatcta ttagcgctac ttaatataaa ttaactaaca atcagtattt tttatgaatt
     5161 ttatacgtgg ttaatactgg ttttcgtatg agatcctgag ttggtggttc aattactcct
     5221 taatgttcgt aggttatgca taaaaaaggc cgcttacgcg gccttagtga ttacatgttg
     5281 tatcaatgat gatcgacagt atggctgtgc tcgatatctt cattcttgcg gctaaagcgg
     5341 cggcgaacca ccacaaagaa taccggaacg aagaagattg ccagtaccgt tgcggtcacc
     5401 atcccgccca ttacaccggt acctactgcg ttctgcgcgc cggaaccagc accagtactg
     5461 ataaccagcg gcataacgcc gaggataaac gccagcgagg tcatcaggat cggacgtaaa
     5521 cgcatccgca ccgcatcaag cgtcgcttca atcagacctt taccttcttt atccatcaag
     5581 tctttggcga attcgacgat aagtatcgcg ttcttcgccg acaacccaat ggttgtgagc
     5641 agtcctactt ggaagtaaac gtcattggtc aggccacgga aggtggcagc cagcaacgca
     5701 ccgataaccc ccagcggaac gaccagcata acggagaacg gagtcgacca gctctcgtac
     5761 agcgccgcca gacacaggaa tacgacaatc aacgaaatcg cgtacagtga aggtgcctgg
     5821 ttgccggaga gacgttcctg ataggacatc cccgtccagt catagccaac accggtaggc
     5881 agtttgctcg ccagttgttc catcagctcc attgcttcac cggtactttt acccggtgcc
     5941 gcctggccta agatttccat ggatggcagg ccgttgtaac gttccagacg cggcgaaccg
     6001 tactcccaac gagaagagga gaacgccgag aatggcacca tctgaccatc agcagcacga
     6061 acataccagt cgccgatatc atccggcagc atacggtatt tcgcttctga catgacgtaa
     6121 actttcttca cacgaccgcg gtcgataaag tcgttcacat agctaccgcc ccatgcagcg
     6181 cccagagtgg tgttaatgtc gttgatagaa acacccagcg cctgcgcttt ttcctggtcg
     6241 atatcaatct taaactgcgg ggtatcttcc agaccgtttg gacgtacgct ggtcagcata
     6301 tcagggtgct tcgctgcttc tgcaagcagc tggttacgcg cctgagtcag tttttcgtga
     6361 ccaaggccag cctggtcaat cagctcaaag tcaaagccgg ttgcagtacc cagttccacg
     6421 attgcgggca ggttaaaggc gaaaaccatc gcatctttga tttgcgagaa agcgcgtgtt
     6481 gcacgcatgg taatcgcttc aactttgttt tcttcgcccg gacgatcggc ccagtccttc
     6541 aaggaaacga acgcaatacc ggtattctga ccacgtcccg caaagccgaa gccgttaacg
     6601 gcgaacaccg actcaacgtt gttcttttct ttggtcagat agtaatgcgt tacctcattg
     6661 agcactttct gcgtacgttc ctgcgttgca cctgctggca gctgaaccat ggtcataaat
     6721 acgccctggt cctcatctgg caagaaggag cttggcagac gcacgaacag ataggccatg
     6781 ccgaccacga tgatcagata cagcactagg taacgccccg tactgcgcag aataccgcct
     6841 acgctgtcgg tgtagtggtg cgtgctcttc tcgaacatgc ggttcaacca gccgaagaag
     6901 ccttttttac cttccccgtg atcgcctttg gcaatcggtt tcagcatggt ggcacaaaga
     6961 gccggagtca ggatcaacgc taccagtacc gacagcgcca ttgctgaaac aatggtaata
     7021 gagaactgac gatagatagc accagtagaa ccgccaaaga aggccatcgg tacgaatacc
     7081 gccgacagta ccatcgcgat accgaccaga gcgccctgaa tctgccccat cgacttacgg
     7141 gtagcttctt ttggcggcaa accttcttcc gccataacac gctccacgtt ttctaccaca
     7201 acgatagcgt catccaccaa caagccgatg gcgagcacca tcccgaacat tgttagcgtg
     7261 tttatcgaga agccaaaggc ggcaaggacg gcaaaggtcc cgagcaatac caccggtacg
     7321 gcaatggtcg gaatcaacgt cgcgcggaag ttctgcagga acagatacat aaccaggaac
     7381 acgaggatga tcgcttcgac cagcgtttta accacttcgt gaatagagat tttcacgaac
     7441 ggcgtggtgt cgtacgggta aacaattttc agacccgacg ggaagaacgg ttccatcttc
     7501 gccagttcag cacggattgc cgcagcggta tccagcgcgt ttgcaccggt cgccagcttg
     7561 atccccagac cggaagcagg ttggccgtta aactctgcga tgatgtcgta gttctcacca
     7621 cccagctcaa tcttcgccac atcacgcagc agtacgcggg aaccatcctg attcactttc
     7681 agcaggattt tgccgaactc ttcagtagag gtcagacgcg tctgagcaat aatagaggcg
     7741 ttaagctgtt ggcctttcac cggcggcgta ccaccgagct gacccgccgc aacctgggcg
     7801 ttctgcgctt tgatggcggt aatgacatca accggcgtta gctggaattt gttcagctca
     7861 ttcgggttca tccagatacg catcgcgtac tgtgaaccga acaactgaac atcacccacg
     7921 cccgacgtac ggctgatggc atctttcata ttcgccgcta cgtagtcaga gatatcctcc
     7981 tgcgtcatgg tgccatcggt gttgataacg ccgacaacca tcaggaagct gctggatgat
     8041 ttctcaacgc tcaccccttg ctgctgaact tcttgcggca gcaacggcat cgccagctgc
     8101 agtttgttct gcacctgaac ctgcgcgata tccgcatcag taccagactc aaaggtcagg
     8161 gtgatctgca cggtacccgt ggagtcactg ttagaggaca tgtacatcag gttatcgata
     8221 ccgttcatat tctgttcgat aacctgtgtc accgtgtcct gcactgtttt cgcatcagcg
     8281 ccggggtagg aggcggagat cgttactgcc ggcggtgcaa tcgtaggata ttgcgccacc
     8341 ggcagtttga ggatcgccag cccccctgcc aacatgatga taatggcgat cacccacgca
     8401 aaaatcgggc gatcgataaa gaaattaggc atgtcttaac ggctcctgtt taagttaaga
     8461 cttggactgt tcaggctgag caccgcttgc ggcttgctgg ttattatcag cggtaacttc
     8521 ttgtgctttt acctggacac caggacgcac tttctgcagc ccacttatta ctacgcgatc
     8581 gcctgctttc agaccttctg tcaccagcca cttatcgcca atagcctggc ttgcaacgat
     8641 cggacgggtt tccactttgt catccgcgcc aactaccagt acggtggcat caccacgcgg
     8701 cgtacgggtt acgccctgtt gcgggactaa aatagcgttt ggattaagcc cttcttccag
     8761 acgtgcgcgc acgaacatac ccggcagcag agtgtgatcc gggttcggga agatagcgcg
     8821 tagggtgata gacccagtgg tctgatcaac ggtaacgtca gagaattcca gcgtaccgtc
     8881 ctgcgggaac ttaatgccgt cactggtgat cagcgacact ttggctttgc cgttctcttg
     8941 tttcagcgtg ccattcgcca gttcctgttt caggcgaagg aagtcgttgc tggactgggt
     9001 cacatcaacg tagatcggat caagttgctg cacggttgcc agcacagtcg cctgaccgtt
     9061 ctgtaccaat gcgccttccg tcacgttcga cttaccaatg cgaccgctaa tcggagaggt
     9121 gactttggtg taagccagat tgatccgcgc agtttcaacg gcagctttcg ccgcagttac
     9181 cgcagcattc gcctgttgcg catcagccag agcctgatcg tacccttgct tactgatgta
     9241 ctgagtaccg agcagtttct gataacgatt caccgtcaat tgcgcgatat tggctgcagc
     9301 ctgggctttc gccagatcac ctttcgcact gtcgtatgtc gcctgatagg tcgcaggatc
     9361 aatctgatag agagagacac cagcttcgat gtcgctacct tctttgaaat tacgcttcag
     9421 gataatcccg ctaacttgag gacgaacttc tgcgatccgg taggcactgg tgcgacctgg
     9481 aagctcggtt gtgatctgca gaggttcagt tttgactgtt actacgccaa cggcgggcat
     9541 ctgctggcca ccttgttggg cctgtttgtc gtcacatcct gttagggcta agctgcctga
     9601 gagcatcaga acgaccgcca gaggcgtaaa ccctctgttt ttgttcatat gtaaacctcg
     9661 agtgtccgat ttcaaattgg tcaatggtca aaagttaata aacccattgc tgcgtttata
     9721 ttatcgtcgt gctatggtac atacattcac aaatgtatgt aaatctaacg cctgtaaatt
     9781 cacgaacata tggcacgaaa aaccaaacaa gaagcgcaag aaacgcgcca acacatcctc
     9841 gatgtggctc tacgtctttt ctcacagcag ggggtatcat ccacctcgct gggcgagatt
     9901 gcaaaagcag ctggcgttac gcgcggtgca atctactggc attttaaaga caagtcggat
     9961 ttgttcagtg agatctggga actgtcagaa tccaatattg gtgaactaga gcttgagtat
    10021 caggcaaaat tccctggcga tccactctca gtattaagag agatattaat tcatgttctt
    10081 gaatccacgg tgacagaaga acgacgtcga ttattgatgg agattatatt ccacaaatgc
    10141 gaatttgtcg gagaaatggc tgttgtgcaa caggcacaac gtaatctctg tctggaaagt
    10201 tatgaccgta tagaacaaac gttaaaacat tgtattgaag cgaaaatgtt gcctgcggat
    10261 ttaatgacgc gtcgggcagc aattattatg cgcggctata tttccggcct gatggaaaac
    10321 tggctctttg ccccgcaatc ttttgatctt aaaaaagaag cccgcgatta cgtcgccatc
    10381 ttactggaga tgtatctcct gtgccccacg cttcgtaatc ctgccactaa cgaataaccc
    10441 tgaatctgac tccaggattt ttcctggaca ttttcgtcgt tgctattctg gttcactgcg
    10501 tcgtgatatt cttgcggttt gactttttca ggtcgttctt caggttcaga aaccttcatt
    10561 catcatgact atgttccagt attacaaacg atcacggcat tttgtttttt cagcatttat
    10621 tgcttttgtt tttgtcttgt tatgccagaa cacggcgttt gcgcgggcgt catcgaatgg
    10681 tgatctgccg acaaaagcgg acctgcaggc gcaacttgac tcactaaata aacaaaaaga
    10741 tctttctgct caggacaaac tggtgcagca ggatctgaca gatacattag ccaccctcga
    10801 taaaatcgat cgcataaaag aagagacagt tcagctacgg caaaaagtcg ctgaagcgcc
    10861 ggaaaaaatg cgccaggcga ccgcggcgtt aacagcactt agcgatgtcg ataacgacga
    10921 agaaacgcgc aaaattctga gtacgctttc gttgcgccag ctggaaactc gcgttgccca
    10981 ggcgctggac gatttgcaaa acgcacaaaa cgatctggcg tcttataaca gccagctggt
    11041 ttcgttacag acgcagcccg aacgcgtgca aaatgcgatg tataacgctt cgcagcagct
    11101 gcaacaaatt cgcagtcgtc tggatgggac tgatgtcggc gagactgcct tacgtcccag
    11161 ccagaaagtg ttaatgcagg ctcagcaggc gttgctgaat gcggagattg accagcagcg
    11221 taaaagcctg gaagggaaca ccgtcttgca ggataccttg caaaagcaac gtgattacgt
    11281 gacggcgaac agcgcccgtc tggagcacca gttgcagctg ttgcaagaag cggtaaacag
    11341 caagcgcctg actttaactg aaaaaacggc gcaggaagcc gtctccccgg atgaagccgc
    11401 gcgtattcag gctaatccgc tggtgaaaca ggaactggaa attaaccagc agttaagtca
    11461 gcgtctgatt accgcgactg aaaacggtaa tcagttgatg cagcaaaaca ttaaagtcaa
    11521 aaactggctg gagcgggcgc tgcaatcgga acgcaatatt aaagagcaga ttgccgtcct
    11581 gaagggcagc ctgctgttgt ctcgtatcct ttaccagcaa caacaaacgc tgccctcggc
    11641 ggatgaactg gaaaacatga ccaaccgcat cgcggatttg cgtctcgaac agtttgaagt
    11701 taaccagcag cgtgatgcac tcttccagag cgatgcgttc gtcaacaaac tggaagaagg
    11761 tcacaccaac gaagtcaaca gcgaagttca cgatgcgtta ttgcaagtgg ttgatatgcg
    11821 tcgcgaattg ctggatcaac tcaacaaaca gttgggtaac cagctgatga tggccattaa
    11881 cctgcaaatc aaccagcagc agttaatgag cgtatcgaaa aacctgaaat ccatcctgac
    11941 tcagcaaatc ttttgggtga acagtaaccg tccaatggac tgggactgga tcaaagcgtt
    12001 cccgcaaagc ctgaaagatg aatttaagtc gatgaaaatc acggtgaact gggaaaaagc
    12061 ctggcctgcc gtttttatcg ctttcctcgc cggtttgccg ctgctgttga ttgccgggct
    12121 gatccactgg cgtctgggct ggctgaaagc gtatcaacag aaactggctt ccgctgtggg
    12181 ttccctgcgt aacgatagcc agctcaacac accaaaagcg atccttatcg acctgatccg
    12241 cgcgctgccg gtgtgcctga ttattctcgc ggttggcctg attctgttga ccatgcagct
    12301 caacatcagc gaactgctat ggtcgttcag taaaaaactg gcgattttct ggctggtgtt
    12361 tggcctgtgc tggaaggtac tggagaaaaa tggcgttgcc gtgcgtcact tcggcatgcc
    12421 ggagcagcag accagccact ggcgtcggca aattgtccgc atcagtctcg cgttgctgcc
    12481 tatccatttc tggtctgtgg tggcagaact ttccccgctg catctgatgg atgatgtgct
    12541 ggggcaggcg atgattttct tcaacctgct gctgattgcc ttcctggtat ggccaatgtg
    12601 ccgcgaaagc tggcgtgata aagagtcgca caccatgcga ctagtcacca ttaccgtgct
    12661 gtcgattatc ccgattgcat tgatggtgct gactgccacg ggttatttct acaccacgct
    12721 gcgcctctcc gggcgttgga ttgaaaccgt ttatctggtg atcatctgga acttgctcta
    12781 ccagacggta ttgcgcggct taagcgtagc ggcgcggcgt atcgcctggc gtcgtgcgct
    12841 ggcgcgtcgg cagaatctgg tgaaagaggg cgcagaaggt gccgaaccgc cggaagaacc
    12901 caccattgca ctggagcaag ttaaccagca gacgctgcgt attaccatgt tgctgatgtt
    12961 tgcgctgttc ggtgtcatgt tctgggcaat ttggtccgat ttgatcaccg tgttcagcta
    13021 tctcgacagc atcacgctct ggcattacaa cggcactgaa gctggcgctg cggtggtgaa
    13081 aaacgtcacc atgggcagtc tgttgtttgc gattatcgcc tcaatggtgg cctgggcgtt
    13141 gattcgcaac ctgcctggtt tactggaagt gctggtgctc tcgcgactga atatgcgcca
    13201 gggcgcgtcg tatgccatta ctaccatcct taactacatc attattgccg ttggtgcgat
    13261 gacggtgttc ggatcgctgg gcgtctcttg ggataaactc cagtggctgg ccgcagcatt
    13321 atccgtaggt cttggttttg gtttacaaga aattttcggt aacttcgtct ccggtttgat
    13381 cattctgttc gaacgtccgg tgcgtattgg cgatacggta accattggta gcttctcggg
    13441 gacggtaagt aagatccgta ttcgtgcgac aacgattacc gatttcgatc gcaaagaagt
    13501 gatcatcccg aacaaagcgt ttgttaccga gcgtctgatc aactggtcgt tgactgacac
    13561 tactacgcgt ctggtgatcc gtctcggcgt ggcctatggc tccgatctgg aaaaagtgcg
    13621 taaagtgtta ctgaaggcgg cgactgagca cccaagggtg atgcacgaac caatgccgga
    13681 agtcttcttt acggcatttg gtgccagcac gttggatcat gagctgcgtc tgtatgtgcg
    13741 tgaactgcgt gaccgtagcc gtactgtcga tgagctgaac cgtactatcg atcagctgtg
    13801 ccgtgaaaac gacatcaaca ttgcctttaa ccagcttgaa gtgcatctgc acaacgagaa
    13861 gggcgatgag gtgacggaag taaaacgcga ctacaaaggc gacgatccga caccagcggt
    13921 tgggtaataa ccaaaggggg cgacatttag ttgccccgag attactgaca aagtgcgcgt
    13981 tgttcatgcc ggatgcggcg tgaccgcctt atccggccta cgaaaccgca agaattcaat
    14041 atattgcagg agcggtgtag gcctgataag cgtagcgcat caggcagttt tgcgtttgcc
    14101 cgcaatctta ggggacattt agcggcccca tttatttctc acttttccgc ctcatcatcg
    14161 tgcgttaatt tcttttcata atcacgcttt acaatatcca gcgcgcgcag cacggtactg
    14221 gcagggatct gattttcctc cagcagcaca atcaaatcga cagccagttt gacatcgtca
    14281 ggggcatttt caagtgacat actctttcca ttgctagcgg gttaagcgcg ctagcctgtt
    14341 ttcgattttt tccagcgcat ggcggcagcg tgccaggcgc gcttcatagg cttccacttc
    14401 acgatgcagc gtttgctgtt ccacgagatc ggtcacccgc gccaggcggg ctctgcgttc
    14461 ggcaaccatc tcacgtagcc gccgctcaaa atcctgatgc tgaatacgtt tacgctgcca
    14521 gcgggaaatt ttcggtggcg cactgtccca ctcgcgcaat gaccaggcgg aggcttcacg
    14581 cgcgatggct tccagttgtg ccgccagatg ttccgccagc caggccactt gcggcagctg
    14641 ttgctgctca actgcatgac gaagcgcagc cagattatcg cccgcctcgt cgagacaaga
    14701 ctgtagtgtt gtcgcacgag tctgaaaaag atgcctgtcg aaacgagcac ttagcgtggc
    14761 gaactgtgac accggggcac aacgctgacg cagcgtagcg agctgtcctt ccagtttttc
    14821 cagcagcagg gcggttttca cgataagacc tcaatgaaaa tgataattgt tatgctaaag
    14881 tagccactat gtaagctgac aacactcaat atgcaacgaa tcattttaat catcattggc
    14941 tggctggcgg tagtgctggg tacgctgggc gtggtattac cggtattacc gacgacgccg
    15001 tttatcctgc tggcggcctg gtgctttgcc cgttcttccc cgcgctttca cgcctggttg
    15061 ctgtaccgct catggtttgg cagctatcta cgtttctggc agaaacatca tgcgatgccg
    15121 cgcggcgtca aaccgcgggc gattttgctt attttgctca cgtttgccat ttctctgtgg
    15181 ttcgtccaga tgccatgggt gcgcatcatg ttgctggtaa ttctcgcctg tttgcttttc
    15241 tatatgtggc gaattccggt gattgatgaa aagcaagaaa agcactgaag cacaacaatc
    15301 gcagttgcaa ttattgcgta cagccagtac attctggcgt tttcgagcac aggcgcaggc
    15361 ggtcaaaggt taaacaacag ttacttttga tacgtttaaa acgcgccgtg agtaccaccg
    15421 taacaagcag gcatacactt atgaccgcga ctgcacagca gcttgagtat ctcaaaaata
    15481 gcatcaaaag cattcaggac tacccaaaac ccggcattct tttccgcgat gtcaccagct
    15541 tactggaaga cccgaaagct tacgctctca gcatcgactt gctggttgag cgttacaaaa
    15601 atgcgggcat taccaaagtt gtcggcaccg aagcgcgtgg cttcttgttt ggcgctccgg
    15661 tagctctggg tctgggcgtt ggctttgtac cggtccgtaa accgggcaaa ctgccgcgtg
    15721 aaaccatcag tgaaaattac gacctggaat acggcaccga tcagctggaa atccacgttg
    15781 atgccatcaa accgggcgac aaagttctgg tggtggacga tctgctggca accggcggca
    15841 ctatcgaagc gaccgttaaa ctgatccgtc gtctgggtgg tgaagtggct gacgctgcgt
    15901 tcattatcaa cctgtttgat ctcggcggcg aacagcgtct cgaaaaacag ggcattacca
    15961 gctacagcct tgtcccgttc ccgggccatt aattatcgcc agtcttgtgc tgcccacgct
    16021 acggacagca caagatgtgc attcagcctc gccgttctga cggggctgtg ttagcattac
    16081 cccttcgtga atccaccttc cagcgtttca gagcctgcca atgagttatc aggttttagc
    16141 ccgaaaatgg cgcccacaaa cctttgctga cgtcgtcggc caggaacatg tgctgaccgc
    16201 actggcgaac ggcttgtcgt tagggcgtat tcatcatgct tatctttttt ccggcacccg
    16261 tggcgtcgga aaaacctcta tcgcccgact gctggcgaag gggctaaact gcgaaaccgg
    16321 cattaccgcg acgccgtgcg gcgtgtgcga taactgtcgt gaaatcgagc aggggcgctt
    16381 tgtcgatctg attgaaatcg acgccgcctc gcgcaccaaa gttgaagata cccgcgacct
    16441 gctggataac gtccagtacg ctccggcgcg tggtcgtttc aaagtttatc tgatcgacga
    16501 agtgcatatg ctgtcgcgcc acagctttaa cgcactgtta aaaacccttg aagagccgcc
    16561 ggagcacgtt aagtttctgc tggcgacgac cgatccacag aaattgccgg tgacgatttt
    16621 gtcacgctgt ctgcaatttc atctcaaggc gctggatgtc gagcaaattc gccatcagct
    16681 tgagcacatc ctcaatgaag aacatatcgc tcacgagccg cgggcgctgc aattgttggc
    16741 gcgcgccgct gaaggcagcc tgcgagatgc cttaagtctg accgaccagg cgattgccag
    16801 cggtgacggc caggtttcaa cccaggcggt cagtgcgatg ctgggtacgc ttgacgacga
    16861 tcaggcgctg tcgctggttg aagcgatggt cgagggcaac ggcgagcgcg taatggcact
    16921 gattaatgaa gccgctgccc gtggtatcga gtgggaagcg ttgctggtgg aaatgctcgg
    16981 cttgttgcat cgtattgcga tggtacaact ttcgcctgct gcacttggca acgacatggc
    17041 cgccatcgag ctgcggatgc gtgaactggc gcgcaccata ccgccgacgg atattcagct
    17101 ttactatcag acgctgttga ttggtcgcaa agaattaccg tatgcgccgg accgccgcat
    17161 gggcgttgag atgacgctgc tgcgcgcgct ggcgttccat ccgcgtatgc cgctgcctga
    17221 gccagaagtg ccacgccagt cctttgcacc cgtcgcgcca acggcagtaa tgacgccaac
    17281 cctggtgccg ccgcaatcag cgccgcagca ggcaccgact gtaccgctcc cggaaaccac
    17341 cagccaggtg ctggcggcgc gccagcagtt gcagcgcgtg cagggagcaa ccaaagcaaa
    17401 aaagagtgaa ccggcagccg ctacccgcgc gcggccggtg aataacgctg cgctggaaag
    17461 actggcttcg gtcaccgatc gcgttcaggc gcgtccggtg ccatcggcgc tggaaaaagc
    17521 gccagccaaa aaagaagcgt atcgctggaa ggcgaccact ccggtgatgc agcaaaaaga
    17581 agtggtcgcc acgccgaagg cgctgaaaaa agcgctggaa catgaaaaaa cgccggaact
    17641 ggcggtgaag ctagcggcag aagccattga gcgcgacccg tgggcggcac aggtgagcca
    17701 actttcgcta ccaaaactgg tcgaacaggt ggcgttaaat gcctggaaag aggagagcga
    17761 caacgcagta tgtctgcatt tgcgctcctc tcagcggcat ttgaacaacc gcggtgcaca
    17821 gcaaaaactg gctgaagcgt tgagcatgtt aaaaggttca acggttgaac tgactatcgt
    17881 tgaagatgat aatcccgcgg tgcgtacgcc gctggagtgg cgtcaggcga tatacgaaga
    17941 aaaacttgcg caggcgcgcg agtccattat tgcggataat aatattcaga ccctgcgtcg
    18001 gtttttcgat gcggagctgg atgaagaaag tatccgcccc atttgatcgt aagcacagct
    18061 tacgttcgtc atccttaacg tgattgagag agaaacctat gtttggtaaa ggcggtctgg
    18121 gtaacctgat gaagcaagcc cagcagatgc aagaaaaaat gcagaaaatg caggaagaga
    18181 tcgcgcagct ggaagttacc ggcgaatctg gcgcaggtct ggtaaaagtg accatcaacg
    18241 gtgcacacaa ctgccgtcgc gtagagatcg acccgagcct gctggaagac gacaaagaga
    18301 tgctggaaga cctggtggct gcagcattca acgacgcagc acgtcgtatt gaagaaacgc
    18361 agaaagaaaa aatggcctct gtatcctccg gaatgcagct gccgcctggc tttaagatgc
    18421 cgttctgatg caaaccagcc cgctgttaac acagcttatg gaagcactgc gctgtctgcc
    18481 gggcgttggc ccgaagtcgg cgcagcgtat ggcgttcacg ctgcttcagc gcgatcgtag
    18541 cggcgggatg cgtctggcgc aggcgctcac ccgggcgatg tcggaaatcg gccactgcgc
    18601 cgattgccgc actttcaccg aacaggaagt ctgtaacatc tgttcgaatc cgcgtcgtca
    18661 ggaaaacggt caaatctgtg tggtggagag tccggcggac atctacgcca ttgagcagac
    18721 ggggcagttt tctggtcgtt attttgtgtt gatggggcat ctgtcaccgc tggacggcat
    18781 cggtccggat gatatcgggc ttgatcgtct ggaacagcgt ctggcagagg aaaaaatcac
    18841 tgaagtgatc ctcgccacca atcccacggt tgaaggtgaa gctaccgcta actacattgc
    18901 cgagctttgc gcgcaatatg acgtggaagc cagccgaatc gctcatggcg taccggttgg
    18961 cggcgaactg gaaatggtcg atggcaccac gctgtcacac tccctcgccg ggcgtcataa
    19021 gattcgtttt taagcaaacg agagcaggag cacctgctct cgcttgaaat tattctccct
    19081 tgtccccatc tctccctcat cctgttttta accttaaaat ggcattattg aggtagacct
    19141 acatgaaagg acaagaaact cgtggttttc agtcagaagt gaaacagctt ctgcacctga
    19201 tgatccattc tctctattcc aataaagaaa tcttcctgcg tgagcttatc tctaacgcct
    19261 ccgatgcggc ggacaagctg cgtttccgtg cgctatctaa cccggacctg tacgaaggtg
    19321 atggcgaact gcgcgttcgt gtctctttcg ataaagacaa gcgcacgctg accatctccg
    19381 ataacggcgt ggggatgacc cgcgacgaag tgattgacca tctggggact atcgctaaat
    19441 ccggtaccaa atcattcctc gaatccctgg gttctgacca agcgaaagac agccagctga
    19501 tcggtcagtt tggtgttggt ttctactctg cgtttatcgt ggccgacaaa gtgaccgtgc
    19561 gtactcgcgc ggcaggcgaa aaaccagaaa acggcgtctt ctgggaatcg gctggcgaag
    19621 gtgaatatac cgttgccgat atcaccaaag aagatcgtgg tactgaaatc accctgcatc
    19681 tgcgtgaagg cgaagacgag ttcctcgatg actggcgcgt gcgttccatc atcagcaaat
    19741 actccgacca tatcgcgctg ccggtagaga tcgaaaaacg cgaagagaaa gatggcgaaa
    19801 ccgttatctc ctgggcaaaa atcaacaaag cgcaggcgct gtggactcgt aacaagtcgg
    19861 aaatcaccga tgaagagtat aaagagttct acaaacacat cgcccacgac tttaacgatc
    19921 cgctgacctg gagccacaac cgcgttgaag gtaagcagga gtacaccagc ctgctgtaca
    19981 tcccgtccca ggctccgtgg gatatgtgga accgtgatca taaacacggc ctgaaactgt
    20041 acgttcaacg cgtgtttatc atggacgacg ctgaacagtt catgccgaac tatctgcgct
    20101 tcgtgcgtgg tctgattgac tccagcgatc tgccgctgaa cgtttcccgt gaaatcctcc
    20161 aggacagcac ggtaacgcgt aacctgcgca atgcgctgac caagcgtgtg ctgcaaatgc
    20221 tggaaaaact ggcgaaagac gacgcggaaa aataccagac cttctggcaa cagtttggcc
    20281 tggtactgaa agaaggtccg gcggaagatt tcgctaacca ggaagcgatc gccaaactgc
    20341 tgcgttttgc ttctacttat accgattctt ctgcgcagac cgtgtctctg gaagactacg
    20401 tttcccgcat gaaagaaggg caggagaaaa tctactacat cactgcagac agctatgcgg
    20461 cagcgaagag cagcccgcac ctggaactgc tgcgtaagaa aggcatcgaa gttctgctgc
    20521 tttccgaccg catcgatgag tggatgatga actatctgac tgagttcgac ggtaaaccgt
    20581 tccagtcggt gtctaaagtt gacgagtcgc tggaaaaact ggctgacgaa gttgatgaga
    20641 gcgcgaaaga agcggagaaa gcactgactc cgttcatcga ccgcgtgaaa gctctgctcg
    20701 gcgagcgcgt gaaagatgtc cgtctgactc accgtctgac cgatacgcca gcgatcgttt
    20761 cgaccgacgc ggacgaaatg agcactcaga tggcgaaact gttcgctgcg gcgggccaga
    20821 aagtgccaga agtgaaatac atcttcgaac tgaacccgga tcacgtactg gtgaaacgtg
    20881 cggcagatac tgaagatgaa gccaagttca gcgagtgggt agaactgctg ctggatcagg
    20941 cgctgctggc agaacgcggc acgctggaag atccgaacct gtttattcgt cgtatgaatc
    21001 agctgctggt ttcctgatgt catgccggat gacctttgtg tcatccggca tttttctttt
    21061 catcatctgt actttccgca aattatctcg ccattaaccg tttcagcctc aggtgccttt
    21121 cttgaggcaa tcgcctgttg gtggtatcgt ttatcgcttt ttcaaaaaaa tcgacacatt
    21181 ttaaggggat tttcgcaatg cgtatcattc tgcttggcgc tccgggcgcg gggaaaggga
    21241 ctcaggctca gttcatcatg gagaaatatg gtattccgca aatctccact ggcgatatgc
    21301 tgcgtgctgc ggtcaaatct ggctccgagc tgggtaaaca agcaaaagac attatggatg
    21361 ctggcaaact ggtcaccgac gaactggtga tcgcgctggt taaagagcgc attgctcagg
    21421 aagactgccg taatggtttc ctgttggacg gcttcccgcg taccattccg caggcagacg
    21481 cgatgaaaga agcgggcatc aatgttgatt acgttctgga attcgacgta ccggacgaac
    21541 tgattgttga tcgtatcgta ggccgccgcg ttcatgcgcc gtctggtcgt gtttatcacg
    21601 ttaaattcaa tccgccgaaa gtagaaggca aagacgacgt taccggtgaa gaactgacta
    21661 cccgtaaaga cgatcaggaa gaaaccgtgc gtaaacgtct ggttgaatac catcagatga
    21721 ctgcaccgct gatcggctac tactccaaag aagcggaagc gggtaacacc aaatacgcga
    21781 aagttgacgg taccaagcag gttgctgaag ttcgcgctga tctggaaaaa atcctcggct
    21841 aatcaagccg cagcagatct gctttcgggc aggtctgctt atccacctca ggcaatacct
    21901 ctcacagcaa ttagttcttc ttcctcactt ttccgctaca attatcaaca agttgaatcg
    21961 ataagaggcg gtaatgcgtc agactaaaac cggtatcctg ctggcaaacc tgggtacgcc
    22021 cgatgccccc acacctgaag cggtaaaacg ctatctgaaa caatttttaa gcgacagacg
    22081 cgtggttgat acctcacggt tgttatggtg gccgttgctg cgcggcgtga ttttgccgct
    22141 gcgctcgccg cgtgtggcga agctgtacgc ctctgtctgg atggaagacg gctcgccgct
    22201 gatggtttac agccgccagc aacagcaggc gttggcacaa cgtttaccgg atacgccagt
    22261 ggcgctgggc atgagctacg gctcgccatc actggaaagc tccgtcgatg aactcctggc
    22321 agagcatgta gatcatattg tggtgctgcc gctttatccg caattctcct gttctacggt
    22381 cggtgcggta tgggatgaac tggcacgcat tctggcgcgc aaacgtagca ttccggggat
    22441 atcgtttatt cgtgattacg ccgataacca cgattacatt aatgcactgg cgaacagcgt
    22501 acgcgcttct tttgccaaac atggcgaacc ggatctgctg ctgctctctt atcatggcat
    22561 tccccagcgt tatgcagatg aaggcgatga ttacccgcaa cgttgccgca caacgactcg
    22621 cgaactggct tccgcactgg ggatggtacc ggaaaaagtg atgatgacct ttcagtcgcg
    22681 ctttggccgg gaaccctggc tgatgcctta taccgacgaa acgctgaaaa tgctcggaga
    22741 aaaaggcgta ggtcatatac aggtgatgtg cccgggcttt gctgcggatt gtctggagac
    22801 gctggaagag attgccgagc aaaaccgtga ggtcttcctc ggtgcgggcg ggaaaaaata
    22861 tgaatatatt ccagcgctta atgccacgcc ggaacatatc gaaatgatgg ctaatcttgt
    22921 tgccgcgtat cgctaaagct gagcggtaaa gaactgagcg ccgtcgcgaa gagcctcgtc
    22981 ggcggttttc atcatccgtg aataatgcaa aaaggcatgc agcgtacctg ggtagagttt
    23041 gaactcacag ggctgctgat gcgccgctaa cgtctagtaa agcagacggc tgtcatccag
    23101 cagcggatcg aactccgccc cggcaataaa acagggcgga acttcgcgag tgagatcatt
    23161 attaaacaga cagtaatacg gtgactcgcg gtccgcgtcg ttgcttaaat acgcctcttc
    23221 atacatctgc aaatcctgtt gcgttaagcc atcccagaca ccgcccaaca gacgacgagt
    23281 cacggaatcc cgtaatccgt aaagcccata ccacagcaaa acacccgcaa ttttaccgca
    23341 atcgatctgt ttatcacgca accacaacgc actggcgagc gccagcatgg cacctgcgga
    23401 atcaccggca aagccaatgc gggacatatt gatttgataa tcctccgcct gctggtggaa
    23461 ataacaacaa gcagccacaa tttcctctat cgcttgtgga aaacgcgctt caggtgaaag
    23521 ggtgtaatta ataccaatca ccgtacattg gctgtagctt gccagcaggc gcatgatgcg
    23581 atcgtgggta tcgagattgc cgagaataaa accgcctcca tgcaaataaa atagcgtcgc
    23641 cgggctatct ggctgcggac aaaagagacg tgtttccacc tgcccatatt ttgttggaac
    23701 catgtaagct ctggttgcca tttctggagc gcccgcattc cagaatcggc gctcaagcgt
    23761 gtaatactgt cgttgctcag caatcgttcc cgttgcgggc caggacggta aatccggctg
    23821 aagagtattc acaacggtct tcatttcagc agaaataagg tccagaacag gtagtttgtt
    23881 ttccggcttc atacaaaaac tcctttcaaa ttacgtcatt gtaaggaacc actgccatga
    23941 aaatgcgatc ccgcctgctg atattgaaac tggctgcgtc tcgcgcgctc ccgtcagatt
    24001 gtgttaacat tcgccgctca gttaaccacc cgtaaaaaca accatgaaat ttcccggtaa
    24061 acgtaaatcc aaacattact tccctgtaaa tgcacgcgat ccgctgcttc agcaattcca
    24121 gccagaaaac gaaaccagcg ccgcctgggt cgtaggtatt gaccagacgc tggtcgatat
    24181 tgaagcgaaa gtggatgatg aattcattga gcgttatgga ttaagcgccg ggcattcact
    24241 ggtgattgag gatgacgtag ccgaagtgct ttatcaggag ctaaaacaga aaaacctgat
    24301 tacccatcag tttgcgggtg gcaccattgg taacaccatg cacaactact cggtgctcgc
    24361 ggacgaccgt tcggtgctgc tgggcgtcat gtgcagcaat attgaaattg gcagttatgc
    24421 ctatcgttac ctgtgtaaca cttccagccg taccgatctt aactatctac aaggcgtgga
    24481 tggtccgatt ggtcgttgct ttacgctgat tggcgagtcc ggggaacgta cctttgctat
    24541 cagtccaggc cacatgaacc agctgcgggc tgaaagcatt ccggaagatg tgattgccgg
    24601 agcctcggca ctggttctca cctcatatct ggtgcgttgc aagccgggtg aacccatgcc
    24661 ggaagcaacc atgaaagcca ttgagtacgc gaagaaatat aacgtaccgg tggtgctgac
    24721 gctgggcacc aagtttgtca ttgccgagaa tccgcagtgg tggcagcaat tcctcaaaga
    24781 ccacgtttct atccttgcga tgaacgaaga tgaagccgaa gcgttgaccg gagaaagcga
    24841 tccgttgttg gcatctgaca aggcgctgga ctgggtagag ctggtgctgt gcaccgccgg
    24901 gccaatcggc ttgtatatgg cgggctttac cgaagacgaa gcgaaacgta aaacccagca
    24961 tccgctgctg ccgggcgcta tagcggaatt caaccagtat gagtttagcc gcgccatgcg
    25021 ccacaaggat tgccagaatc cgctgcgtgt ctattcgcac attgcgccgt acatgggcgg
    25081 gccggaaaaa atcatgaaca ctaatggagc gggggatggc gcattggcag cgttgctgca
    25141 tgacattacc gccaacagct accatcgtag caacgtacca aactccagca aacataaatt
    25201 cacctggtta acttattcat cgttagcgca ggtgtgtaaa tatgctaacc gtgtgagcta
    25261 tcaggtactg aaccagcatt cacctcgttt aacgcgcggc ttgccggagc gtgaagacag
    25321 ccaggaagag tcttactggg atcgttaagt tatcgtcggt tcgtaggcca gataaggcgt
    25381 tcacgccgca tctggcattt ggctctcgat gcctgatgcg acgctggcgc gtcttatcat
    25441 gcctacatat ttttcatatt ttacatccgg caaccaccgt ttaccccgtc accacttcac
    25501 ccgccggtgg cgtttccagc agttccagca tagtacgggc aatttcacgt tcacccatca
    25561 ctacctgatt cgcgccacgt tcggtgatat acgtcacttc atcgtcataa tgggcgcggg
    25621 cgataatctc aatatccggg tttttcgcgc gggcagacgc cacaatctca cccgcttcat
    25681 aaccgttggg gatcgtcagg atcagccatt ttgcacattc cagatgtgcc agttgcataa
    25741 tttcttcgtt cgctgcattg cccaatacag cgcggacccc acgctctcgc agctcatcaa
    25801 cacgggttcg tgacgtctca atcaccacca gcggaatatc agaggcgagc aatttctccc
    25861 ccagcaggct gcctacacga ccgtaaccca ccaatagcgc atggttgcaa atatccactg
    25921 ggatctgttt ctcttcttcg attgcctctt ccagcgtctg ctcttccagc gtttcggtct
    25981 tcgccagata tttctccagt agtgcgaaca gtaccgggtt gagcataatc gacaggatcg
    26041 cccctgccag taccaggttt tgtccggcct gcggcagtaa attcaatgcc attcccagtc
    26101 ccgccaggat aaacgcgaac tcaccaatct gcgccaggct ggcggcgatg gttaatgccg
    26161 tacgttggga gtgaccaaac agacggacaa ggaacagggc cgcgaccgat ttaccaaaca
    26221 gaataattgc cagcgtcgcc agcactgcca gcggttgctg aatcagaatt aacggatcaa
    26281 acaacatccc gactgagaca aaaaacagca ccgcaaacgc gtcgcgcaat ggcaacgtat
    26341 cgtgggcggc acggtgactc aattcagact cgttcagtac catcccggca aagaacgcac
    26401 cgagtgcaaa ggagacatca aacagctcta ccgcaccaaa ggcaatccct aacgccagcg
    26461 ccagcactga cagggtaaac agctcgcgag aaccggttgc cgcgctgcgt gccataatcc
    26521 acggcaccag acggcgacct accagcatca taatggcgat aaatgcgatc accttgccga
    26581 tggtgatccc catatcgact gcaagagtgg caaagcccac atcgccctgt tccatcattc
    26641 ctgccactgc gggcagcaac accagcgtca gaaccatcac caggtcttcc acaatcaacc
    26701 aaccgatggc gatttgccca cgctgactgt ctattaattg ccgttcttca agtgcgcgca
    26761 gtaacaccac ggtactggcg gtggaaagac ataagccgaa cacgataccg gtcattaacg
    26821 accagcccag cacggcggag agcgccatac ccagcagcgt cgccacggct atctgggcga
    26881 tcgcgccggg aatggcgatg gactttaccg ccatcaaatc cttgagcgaa aaatgcaaac
    26941 cgacgccgaa catcagcaaa ataacgccca gttcagccag ttccggcgca agcttggtat
    27001 cggcaacaaa gcccggagtg aatggtcctg ccagcacacc cgctaacaga tatcccacca
    27061 gaggagaaat acgtagttta ttggccagca tgccgaggat aaaggcgagc acaaggccgc
    27121 caacaatggt ggtgataagc ggggtggcgt gatgcattcc gtctcctttt cctggtggtt
    27181 attgtccatt tttggctggg aaaaccaaaa ttacaggtaa tagtttatga caatttcatt
    27241 gatgatgttt atgaataatt gttgaatttt gcagaaaaat ggaattagct gcaaaaaaag
    27301 cacggttcgg aaaacagaag gcggtttaac agaggaaagg cttatggcga ctgtaaaacg
    27361 atgcagccaa agtttgcctt tggctgcaat gaaatcagtc tttatgccgg ttatcaggca
    27421 ggaatatggt caacatcccc aatagtggca ggaaagcaca gattttatag actaactcga
    27481 tgctggtgtg atcggcgata agccccagaa ctgccgctcc cagacctccc atgccgaaag
    27541 caaaaccgaa aaagagtcca gaaaccatac cgatacgtcc cggaagtagc tcctgagcgt
    27601 agaccagaat ggcagagaat gccgaagcga ggataaatcc aataatcacc gttaaaaccc
    27661 ccgtccagtg caggcgggcg tagggtaaaa tcagcgtaaa cggcgcaacg ccgaggatag
    27721 agccccaaat cacatatttc cgtccaattt tatcccctac aggcccgccg atcaccgtac
    27781 ctgccgcaac ggcaaacagg aaggcaaaca gatgaagctg ggcattctgg atagataatc
    27841 cgaatttttg catcagataa aaggtgtaat agctgctgat gctcgccata tagaaatatt
    27901 tcgagaaaat gaggattaac agaatgctga ctgccagtac cactttgttg cgcggcagtg
    27961 gattgattat cgtcgctttg ggttttcctt tattcattcg gtgctgtgcc gagtaccaac
    28021 ggctaatttg cgccaacacc acgatcgcca gcagtgccgc aagcacaaac caggcaacgt
    28081 tgcctttgcc ataaggctcg ataatcaccg ccgccagcaa gggtcccagg gaactgccaa
    28141 agttgccgcc gacctgaaag atagattgcg ccaggccatg ccgcccgccg gaagccatac
    28201 gggctacgcg agaagattcc ggatgaaaga ccgatgaacc ggtaccgacc agcgccgctg
    28261 ccagcagaac tgcgccaaaa ctgcccgcca gcgcaagcag caccagacca cttaaggtaa
    28321 agcacatgcc aattggcaac gaccacggca tcggatattt atcggtccag tagccgacca
    28381 ctggttgcag tagcgaagag gcgagctgga aggtgagggt tatcatgcca atctgcataa
    28441 atgtcagaga aaattctgac tgaagcagcg gataaatcgc cagaatcagc gattggatca
    28501 tgtcgttcag cagatgtgag aggctgatag cacctaaaat accaaacgat gttcgggcct
    28561 tggtcgttga cgcagccgcg cccgccacag gctgggtttg ttcactcatt gccataggaa
    28621 agtcactttt tcagggttgc gatgtaaaga atgatcttat ttgtgattat tacccgacta
    28681 acatacctgt atgcgtcgtc tgaaggaagt ctcaacgccg aatacagaat ttctaatctg
    28741 gatgcagatt tatcttcacc ggacgcagac ttgtctatga tgttgcgtca tactattttt
    28801 caacacgttg aaatcaggtc agggagagaa gtatgaaatt attgcagcgg ggcgtggcgt
    28861 tagcgctgtt aaccacattt acactggcga gtgaaactgc tctggcgtat gagcaggata
    28921 aaacctacaa aattacagtt ctgcatacca atgatcatca tgggcatttt tggcgcaatg
    28981 aatatggcga atatggtctg gcggcgcaaa aaacgctggt ggatggtatc cgcaaagagg
    29041 ttgcggctga aggcggtagc gtgctgctac tttccggtgg cgacattaac actggcgtgc
    29101 ccgagtctga cttacaggat gccgaacctg attttcgcgg tatgaatctg gtgggctatg
    29161 acgcgatggc gatcggtaat catgaatttg ataatccgct caccgtatta cgccagcagg
    29221 aaaagtgggc caagttcccg ttgctttccg cgaatatcta ccagaaaagt actggcgagc
    29281 gcctgtttaa accgtgggcg ctgtttaagc gtcaggatct gaaaattgcc gttattgggc
    29341 tgacaaccga tgacacagca aaaattggta acctggaata cttcactgat atcgaatttc
    29401 gtaagcccgc cgatgaagcg aagctggtga ttcaggagct gcaacagaca gaaaagccag
    29461 acattattat cgcggcgacc catatggggc attacgataa tggtgagcac ggctctaacg
    29521 caccgggcga tgtggagatg gcacgcgcgc tgcctgccgg atcgctggcg atgatcgtcg
    29581 gtggtcactc gcaagatccg gtctgcatgg cggcagaaaa caaaaaacag gtcgattacg
    29641 tgccgggtac gccatgcaaa ccagatcaac aaaacggcat ctggattgtg caggcgcatg
    29701 agtggggcaa atacgtggga cgggctgatt ttgagtttcg taatggcgaa atgaaaatgg
    29761 ttaactacca gctgattccg gtgaacctga agaagaaagt gacctgggaa gacgggaaaa
    29821 gcgagcgcgt gctttacact cctgaaatcg ctgaaaacca gcaaatgatc tcgctgttat
    29881 caccgttcca gaacaaaggc aaagcgcagc tggaagtgaa aataggcaaa accaatggtc
    29941 gtctggaagg cgatcgtgac aaagtgcgtt ttgtacagac caatatgggg cggttgattc
    30001 tggcagccca aatggatcgc actggtgccg actttgcggt gatgagcgga ggcggaattc
    30061 gtgattctat cgaagcaggc gatatcagct ataaaaacgt gctgaaagtg cagccattcg
    30121 gcaatgtggt ggtgtatgcc gacatgaccg gtaaagaggt gattgattac ctgaccgccg
    30181 tcgcgcagat gaagccagat tcaggtgcct acccgcaatt tgccaacgtt agctttgtgg
    30241 cgaaagacgg caaactgaac gaccttaaaa tcaaaggcga accggtcgat ccggcgaaaa
    30301 cttaccgtat ggcgacatta aacttcaatg ccaccggcgg tgatggatat ccgcgccttg
    30361 ataacaaacc gggctatgtg aataccggct ttattgatgc cgaagtgctg aaagcgtata
    30421 tccagaaaag ctcgccgctg gatgtgagtg tttatgaacc gaaaggtgag gtgagctggc
    30481 agtaatccga aagtgccgga tgtttgcatc cggcacaatg cttaatcgcg gcgggcgata
    30541 tcagcaaatt tggcatcgag gatctttgcc agatcgcctg ccgccagttc gatatccagt
    30601 ccgcgcttgc cgccggaaac ataaatagtg gcaaattctt gtgcgggggc gtcgataatc
    30661 gttggcagac gttttttctg ccccagtggg ctaatccccc caaccaggta tcccgtcgaa
    30721 cgctgcgcga ccatcggatc ggccatctca actttcttgg cacccagcgc ttttgctact
    30781 tttttaagat ccagttgacc tgcgaccggc gtaacggcca cggcaaggtg tttcatatca
    30841 ccgttcactg ccaccagcag cgttttgtag acctgatccg gattcaaacc taattttttg
    30901 acgacttcat cgccaaaatt ggtttcagcc ggatcgtgct cgtaggtatg gatttgaaac
    30961 gaaatcttgt ttttttcgag taatttaact gcgggtgtca tggtaatttt cctgccttaa
    31021 acaaaaaaac gaagcaagca tacgcctgat ggcggtccaa aaaaaagagt catcttgcct
    31081 aagagtattg gcaggatggt gagattgagc gacaatcgag ttacaccgtc gcttaaagtg
    31141 acggcataat aataaaaaaa tgaaattcct ctttgacggg ccaatagcga tattggccat
    31201 ttttttagcg caacatttgc ggcaaattcc cttctccata caggtgtagt gcaccgaccg
    31261 cgaccacata tcgccccggc ggcatggcgc gtaatttatc ccgccaggcg agatttcgct
    31321 gatgcatcag cacatcgtac agcgactgac tgaacgtatt gggcagcgtt atatcattat
    31381 tttgcggcgg tgcattcagc caccagctca tcatttgttg cagcaaccgt gcgttggtat
    31441 gccagtgggt cagcgtatcg tccagcagcg ccagtccttt gtcagggagc tggagcaaca
    31501 tggcaatctg gttttcagcc ccttccagtt caatcacggg tttatgttgt tgcttcgccg
    31561 cctgcaatag ctggtaatcg ataccgtatt ctgcccgcag ccccagtttt tgtgcctgcg
    31621 tcgcctgaag aaccatcgcg atttgccaca gcggttgggt agaaaagagt gagggagaaa
    31681 tgcccatctc ctggctaatg tgctgcaggt tttggagttg ctcctcgcta atgcgctctt
    31741 ccagcgcctc gcaggcaggc aaattagcaa aaggcgtatc gctggtggaa acatccgcct
    31801 cgacgatcag cgcatcggcg tttttgagct ttttgagcaa acgggtgggc aggggagcca
    31861 tatcgtggct acccatatga atactgccaa tcagatgaaa atggcgattg ccggggaggg
    31921 tgatatcgat ggcaggccag gtgtaatgat taccgcgcag cgcggcccaa agcgttttta
    31981 cccggtacaa cagatccata cgacctccct ttgtgaaata tcatgctagc gcgcggtgag
    32041 ggatggcgca aatggagttg gggacttgtt taatgtgttt gtatgattca gtatgttctt
    32101 gcatcgctat tcacagggaa gcaacagtta aaaaccatga aacaggcaac aagaaaaccg
    32161 acgacacccg gagatattct tctctatgaa tatctggaac cgctcgattt gaaaatcaat
    32221 gagttagcag agttgctgca tgttcatcgt aatcgcgtca gtgcactgat caataacaat
    32281 cgtaaactca ccactgagat ggcattccgt ctggcgaaag tttttgatac cacagtcgat
    32341 tttaggctaa acctccaggc ggcagttgat ctttgggaag ttgaaaacaa catgcgcacc
    32401 caggaagaat tgggacggat tgaaacagtg gctgaatatt tggcacgccg tgaagagcgt
    32461 gcaaaaaagg tcgcgtaagg ccaaaacatc gcttcatctc gtaggccgga taaggcgttc
    32521 acgccgcatc cggcatccga gcaccattgc ctgatgcgac gcttacgcgt cttatcaggc
    32581 ctacaaacct gtgcctgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcagtca
    32641 tgcgtcgatg ccaaatgcgc cgccctaaag cagcgcatcc gcaatgacgt acttattcct
    32701 tcggtttaaa ccgcagcaac cggttggcgt tacttactac ggtaattgac gagagcgcca
    32761 ttgccgctcc ggcaactacc gggttaagca gtgttccagt aaacggccac aaaataccgg
    32821 cggcgaccgg aataccgatg ctgttgtaga taaaggcacc gagcaggttc tgcttcatgt
    32881 tgcgcagcgt tgcgcgggaa atcgcgagcg catccgcaac gcccatcagg ctatggcgca
    32941 tcagggtaat cgccgcggtt tcaatggcaa catcactgcc gccacccatc gcaatgccga
    33001 catccgcctg ggccagcgct ggcgcgtcgt taatgccgtc gcccaccatt gccacctgac
    33061 gtccttcact ttgcagacgt ttgatcgctt cggctttacc gtccggcagc actccggcaa
    33121 tcacttcatc aattccagct tctttggcga tcgcattggc ggtggttggg ttatccccgg
    33181 tcaacatcac cagacgatat cccgctttat gcaggcgttg cagcgccgcc acgctatcac
    33241 tacgcaacgg atcgcgtaca gccagcaggg ctactgcttt cccgtcaatc gccagcagca
    33301 caggcgttgc tccttgcgat gcctgagctg aaatatcggc ttcaatcgct ttagtatcga
    33361 cttgctgatc atttaacaac gcctgattac ccagcaataa cgcatgacct tctgcttcac
    33421 cgctcacgcc cagcccgcgc aatgtgcgga aaccgttgac ctgcggtagc tgcatatcgc
    33481 ctgctttatc aaggatcgct cgtgccaatg gatggctgga accttgctcc agcgccgccg
    33541 ccagacgcag cgcctgcgct tcgtcaacat cagcaaatgt tttcactgcg acaacctgcg
    33601 gcttcccttc ggtcagcgtc ccggttttat cgaacacgac ggtatcgagt gtactggcgc
    33661 gttgcagcgc gtcggcgtcc cgcaccagca cgccaaactc agccgcccgc ccgacgccgg
    33721 aaataatcga catcggcgtc gccagtccca gcgcacacgg gcaggcgata atcagcaccg
    33781 tagtggcaat caccagggta tagacaatct gcggtgccgg accaaagaaa taccagattg
    33841 ccgcactgac aagcgcaata accactacta ccggcacaaa tatggctgag attttatccg
    33901 ccagctgacc gatttctggc ttgctgctct gggcctggcg caccatgcga atgatccttg
    33961 atagcgtggt atggctgcca accgcactgg cacgaaacag cacactgccg tcctgtacca
    34021 ctgtcccggc atggacgcta tcgccttcgc ctttttgctg cgggattggt tcgcccgtca
    34081 gcatcgcttc atccagccat gcttcgccct gggtaatctc gccatctacc ggcacgcgat
    34141 cgccggtcgt caggcgcagc aacatacctg gctgcacttc tgccagaggc acgctttttt
    34201 caccttcgtc agtaaccagg cgcgccgtcg gtggggttaa atcgagtaat ttctccagtg
    34261 ccttagaaga acgctgacgt gcgcgtgctt ccagcatatg gccgagattg atcagaccga
    34321 taatcatcgc actggcttca tagtaaagat gtcgcgcttc catcgggaac cactgcggcc
    34381 acaggttgac gctcatcgaa tagagccacg ccacgcccgt tcccagcgcc accagcgtat
    34441 ccatcgtcgc tgcaccgttc agcaggcttt tccatgcact gcggtaaaaa tggccgctgg
    34501 cgaaaaccat caccgccagg gttatcaggc cgataaccaa ccacaggctg cggttgtcgg
    34561 cggtgactat catgttatcg ccgatcatcc cccagaccat caccgggata cccaccgcca
    34621 gtgcgacaat tgcctgccag cggaagcgct tcatcgtagc gacggcggtt tcttgctggc
    34681 gttcgcggcg tttcgcgtca tcttcaatcg cttccgcgcc gtagcccgct ttttccaccg
    34741 cctgcactaa atcttgtggg gaggcactgc ccatcaccag cgcagtacgc tccgccaggt
    34801 ttacccgtgc ctgagtgacg cccggtacgc tttgcagcgc attttgtacg cgggtgacac
    34861 agctggcgca gctcatgccg ctcagcagca actgctggct gtcatcgtca tcggcggtcg
    34921 ctgccggaag cgcctcagaa accgctgtca gtgcttccga cgggattgat gactccgcca
    34981 gcggtttagc ctttgggtgg cttacagatg cgtcataacc cgcttgtttg atggtttcga
    35041 tcagctgttc tgcactggca gtcccggtaa cgtgcgcttc agtgatagac acatccgctt
    35101 gttccacatc cgggcgctgt tcaagacttt ctttcacgcg tttaacgcag tgaccgcagg
    35161 acaggccgtc caggttcagg tcgatagttt gtgacataaa acactccttt aagacagttt
    35221 tgactggctg tgataaaggt taaaccttcc agcaagggga aggtcaagaa attaataaac
    35281 caggcgggta aaagtccgta aagattaaaa aatcggctcg atttgcatca ggattagaca
    35341 tttatttctt tgttttcctg tagttaagtt gcgggtgcta agttaaatca ggatgcctga
    35401 aaatcggcac tggggtgagg aattacctcc cgcatctata aaaaggagtt aacaaaagat
    35461 gttagatgca aacaaattac agcaggcagt ggatcaggct tacacccaat ttcactcact
    35521 taacggcgga caaaatgccg attacattcc ctttctggcg aatgtaccag gtcaactggc
    35581 ggcagtggct atcgtgacct gcgatggcaa cgtctatagt gcgggtgaca gtgattaccg
    35641 ctttgcactg gaatccatct ctaaagtctg tacgttagcc cttgcgttag aagatgtcgg
    35701 cccgcaggcg gtacaggaca aaattggcgc tgacccgacc ggattgccct ttaactcagt
    35761 tatcgcctta gagttgcatg gcggcaaacc gctttcgcca ctggtaaatg ctggcgctat
    35821 tgccaccacc agcctgatta acgctgaaaa cgccgaacaa cgctggcagc gaattttaca
    35881 tatccaacag caactggctg gtgagcaggt agcgctctct gacgaagtca accagtcgga
    35941 acaaacaacc aacttccata atcgggccat tgcctggctg ctgtactccg ccggatatct
    36001 ctattgtgat gcaatggaag cctgtgacgt gtacacccgt cagtgctcta cgctcatcaa
    36061 tactgttgaa ctggcaacgc ttggcgcgac gctggcggcg ggtggtgtga atccgttgac
    36121 gcataaacgc gttcttcagg ccgacaacgt gccgtacatt ctggccgaaa tgatgatgga
    36181 agggctgtat ggtcgctccg gtgactgggc gtatcgcgtt ggtttaccgg gcaaaagcgg
    36241 tgtaggtggc ggtattctgg cggtcgtccc tggcgtgatg ggaattgccg cgttctcacc
    36301 accgctggac gaagaaggca atagtgttcg cggtcaaaaa atggtggcat cggtcgctaa
    36361 gcaactcggc tataacgtgt ttaagggctg atcatgatga acacggaagg taataacggt
    36421 aacaaacccc tcggtctatg gaacgtcgtt tccatcggta ttggggcaat ggtgggggcg
    36481 gggatcttcg cgctgctggg gcaggctgcg ttgctaatgg aagcctcgac ctggggcgcc
    36541 tttgcttttg gcggtattgt ggctatgttt tccggttatg cctatgcgcg tctgggggcg
    36601 agctatccca gcaatggcgg tattatcgac ttttttcgtc gcggattagg caacggcgtc
    36661 ttttcgctag cgctctcgtt gctatacctg ttgacgctgg cggtgagcat cgccatggtt
    36721 gcccgtgctt ttggcgctta tgccgtgcag tttttgcatg aaggcagcca ggaggagcac
    36781 cttattttgc tctacgcgtt ggggatcatt gcggtgatga cgcttttcaa ctccttaagc
    36841 aaccatgcgg tagggcggct ggaagtgatc ctcgtcggca ttaaaatgat gatcctgtta
    36901 ttgctgatta ttgccggtgt ctggtcgctg caaccggcgc atatttccgt ctctgcgccc
    36961 cccagctccg gtgcgttctt ctcctgtatt gggataacct tccttgccta tgcgggcttt
    37021 ggcatgatgg cgaacgcggc ggataaagtg aaagatccgc aggtcattat gccacgggcg
    37081 tttctggtgg cgattggcgt taccacgttg ctttatatct cgctggcgct ggttttgctt
    37141 agcgatgtat cggcattaga gttagaaaaa tatgctgata ccgccgtagc gcaggctgct
    37201 tctccgctgc tcgggcatgt gggttatgtg atcgtcgtca tcggcgcttt actggcgacg
    37261 gcttcaggca ttaacgcgaa cctgttcgcc gtgtttaaca tcatggacaa catgggcagc
    37321 gaacgcgaac tgccgaagct aatgaataaa tccctgtggc ggcagagtac ctggggcaat
    37381 atcatcgtcg tggtgttgat aatgctgatg acggcggcgc taaatttaga ctcactcgcc
    37441 agcgttgcca gcgccacctt tttgatctgc tatctggcgg tgtttgtggt ggcgatccgc
    37501 ctgcgtcatg atattcacgc ctcgttgccg attcttatcg ttggtacgtt ggtgatgttg
    37561 ttggtgatcg ttggctttat ctacagcctg tggtcccagg gcagccgtgc gttgatatgg
    37621 attattggtg caatcttact cagccttatt gtggcaatgg tcatgaagcg taataaaacc
    37681 gtataacacc tctctgtgcg cagtacttcc tgtattattg tggtggcggt cgatattcgc
    37741 actggcaaaa aaacgtgctt gaatatctgt tgaaaccctt taacaaagca caggaggcgt
    37801 tgcgcgaacg atgaacatca gcgatgtagc aaaaattacc ggcctgacca gcaaagccat
    37861 tcgcttctat gaagagaagg ggctggtgac gccgccgatg cgcagcgaaa acggttatcg
    37921 cacctacacg cagcagcatc tcaacgaact gaccttactg cgccaggcac ggcaggtggg
    37981 ctttaacctg gaagagagcg gcgagctggt gaatctgttt aacgacccgc agcggcacag
    38041 cgccgacgtc aaacggcgca cgctggagaa ggtggcggag atcgaacgac acattgagga
    38101 gctgcaatcc atgcgcgacc agctgctggc actggcgaat gcctgccctg gcgatgacag
    38161 cgccgactgc ccgattatcg aaaatctctc cggctgctgt catcatcggg cagggtgatt
    38221 aagacgagac ggctcggata tgtagggtta tcccttctat cgcaatgact tcaacatgcg
    38281 tacctgcacc gagatcctcg ctggcgctga caggccatga actgtcaccg acgcgcatat
    38341 gaccgcgccc gttgaccagc ggagattcca gcacaaaacg tcggccaatc agctgctgcc
    38401 cgcgctggtt taaatgacta tcgctgtgct tttgttcgcg cacccgccgc gacaaccatt
    38461 tccaccacag ccaggcggcg agcagcgtca ggatggcaaa cattacccct tgccactccc
    38521 aacccagcgg caccagccag accaccaggc cggtaatcac cgctgccacg ccgctccaca
    38581 acaaataacc atttccgccc agcatctcgg ctgccagcag caacccgccg agactgagcc
    38641 agaaaatatg tggatgaacg accattaact ccatcatggc tgagtccgtt tgttggcgct
    38701 gtctttcacc agctcggcaa tcccggcaat cgaccccatc aggctgctgg cctctaatgg
    38761 catcatcact actttgctgt tactggagga accgatctgc tgtaacgctt cggtgtattt
    38821 ttgcgctacg aagtagttca ccgcctgaat atcaccggag gcgatagctt cagacaccat
    38881 tttggtggcg cgggcttctg cttcggcgga acgttcacgc gcttcagcct gtaaaaacgc
    38941 cgactgacgt tcgccttccg ctttcaggat ttgcgactgt ttttcacctt cggctttgag
    39001 gatttccgcc tgacggatcc cttccgcttc aagaatgtaa gcgcgtttgg tacgttccgc
    39061 tttcatctgc gcgttcattg aagagataag ctctgccggt gggcgcacgt cgcgaatttc
    39121 aatacgggtg actttaatcc cccacgggtt ggtggcttca tcgacaatac gcagcaggcg
    39181 tgagttgatg ctgtcgcgct gagagagcat ttcgtcaagt tccattgaac ccaacacggt
    39241 acggatgtta gtcatggtca ggttgatgat cgccagctcc agattgctga cttcataggc
    39301 agcgcgtggc gcgtcaatca cctgaataaa acagactgcg tcgatggtaa cgttggcgtt
    39361 atctttcgag ataacttcct gggaagggat atcgagcact tgctccatca tattgatctt
    39421 gcgaccaatg cgatccataa acggcaccac cagactgagc cccggctgta acgttttggt
    39481 atagcgacca aaacgttcta ccgtccattg atagccctgc ggcacgattt tgacgcccgc
    39541 gccgacaatg accagcgcga caaaaatgag aatcgggata aagataagca tcggaaaaac
    39601 ctcctgttgt accgtccata atcagcaaaa ttgctgcttg attaaacaaa ttatacctga
    39661 ttactgaaag agagttcccc cttattcctg cgaaggataa actgttttta gtaaaaatca
    39721 gaaaaaggga acagcgatgc aggaaaatag tcctttgctt cagctacaaa acgtaggata
    39781 tctggcgggt gatacgaaga ttcttaataa catcaatttt tcgctgcgtg ctggcgaatt
    39841 taagttaatt accggtcctt ctggttgtgg caaaagtacg ctgctaaaaa tagttgcttc
    39901 attgatcagc ccaaccagcg gaacgttact gtttgaaggt gaggatgtca gcacactaaa
    39961 accagaaatc taccgccagc aagtctctta ctgcgcccag acgccgacgc tgtttggcga
    40021 tacggtatac gataatctga tctttccctg gcagatccgt gaccagcagc ctgacccggc
    40081 catttttctc gattttctcg aacgcttcgc tttgccggac agcattttga cgaagaatat
    40141 cgccgagcta tctggtggtg aaaaacaacg catctcattg attcgtaacc tgcaatttat
    40201 gccgaaggtt ttattgctgg atgaaataac cagtgcgctg gatgaaagta ataaacataa
    40261 cgtcaatgag atgatccatc gttatgtgcg cgagcaaaat attgccgtgc tgtgggtgac
    40321 acacgataaa gacgaaatta atcatgcgga taaagtgatt acactgcaac cgcatgccgg
    40381 agaaatgcag gaagcacgct atgaactcgc ataatattac taacgaatca ttagcactgg
    40441 cattaatgct ggtggtggtg gcaatcttaa ttagccataa agaaaaactg gcgctggaga
    40501 aagatattct atggagcgtc gggcgagcga taattcagct gattattgtc ggctatgtgc
    40561 tgaagtatat tttcagcgtg gatgatgcca gcctgacatt attgatggtg ttatttatct
    40621 gctttaatgc ggcgtggaac gcgcaaaaac gcagtaaata tattgctaaa gcttttatct
    40681 catcgtttat tgccattacg gtcggggcgg gaattaccct ggcggtgctg attctctcag
    40741 gggcgattga atttatcccg atgcaggtga tccctgtcgc cgggatgatt gccggtaacg
    40801 ccatggtagc ggtggggttg tgttacaaca atttagggca acgggtcatt agcgaacagc
    40861 aacagattca ggagaaactg agtcttggcg cgacgccgaa gcaggcttca gcgatattga
    40921 ttcgcgacag tattcgcgcg gctttaattc cgacggtcga ttcagcaaaa acggttggct
    40981 tagtgagttt accaggaatg atgtccgggc tgatatttgc cgggattgat ccggtgaagg
    41041 cgattaaata tcagattatg gtgaccttta tgctgctctc aaccgccagc ttgtcgacca
    41101 ttattgcctg ctatttaacc tatcgtaagt tttataattc gcgccaccag ttggtggtga
    41161 ctcaattgaa gaagaaatga tgcgatgccg gatgcgcagc atcgcatccg gcgttgtggt
    41221 tcatgtgccg aacaacggaa tatcaataca acaatgcata cagctggcgg cgatacttcg
    41281 acgccagtgc atcacccgta cccagcgcag cgaggatctc ctggaacgtt ttacgcgtct
    41341 gaccgtctgc ggcggtgaga tctttacgca gatgcccgaa cagcaactcc agcgcctctt
    41401 cattgcgccc aacctgatgc agttgcagcg ccagttgcgt cgccagtgcg gcatcttctg
    41461 gattctccgc cacctgctgt tgcaactgtt gaatttccgg cttatcagcc gcctgcttca
    41521 gcagttcgat ttgcgctacc agcccctggt agcgggtgtc ctgatcctgc aacggaatgg
    41581 ttttcagcac cgcttccgca tcttcagaac ggttcagcgc aatcagcgtt tctgccagca
    41641 gcaggccgat ctccccgttc tgattcgaca actgccaggc gtctttcagc aatggcaggg
    41701 catcggtgta attgccttcc tgcatcagtt gcatcgcctg ctgcgctttc agctcttctt
    41761 cgcgcggcag cactttatcc agcagggcgc ggatcgcctc ttccggttgc ggcccctgga
    41821 agccatctac cggttgcccg ttctggaaca gatacacggt cggaatcgcg cgcagaccaa
    41881 actgggcggc aatcatctgc tccgcgtcgc agtccagctt cgccagaata aattgcccgt
    41941 tgtactgcgc cgcgaggctt tccaggatcg gggttaactg caaacagtgc tggctacgtt
    42001 cagaccaaaa atagaacagc accggagtgg tcatcgactg ttcaagaacc tgttgcaggt
    42061 tagattcgtt aatgttgaca atattttcta cggacatgga gtcgctctct gttgtcgata
    42121 ttttctttga catgggggct taagcgcgcg cttcaactca cccctgcaat attttgtcca
    42181 tcacgcgccc aggcagcagg cgctcgagca ccattactgc ccaggtcacc agcgttaccg
    42241 gatagcgcat cttcggcttc tcgctaataa aagcatggcg cactttgtcg accaccgctt
    42301 ccggtcccaa cgtaaagcgg gcggcgatgc cgggattttc gactggttta tcactttgcg
    42361 tctggttgac gttgtcggtg aagcgagtac gaatgggacc gggttcgatc aggctgactt
    42421 taattccgct gtggcgtagc tccatgcgca gcgcgtctga ccacgcctcc agcgcatatt
    42481 tactggccgc gtaagcgcca cgacccggcg tggagattaa tcccatcacc gatgatgtca
    42541 tcacaatacg cccttcaccg tgcggtaaca tcgcgggtaa caggcgcatg gtgagctggt
    42601 gtgcgccgaa aaagttggcg gaaaactgct gttccatctg cgcacggctg atggtggaaa
    42661 gggggccata catgccgaat ccggcattgt taaagatccc atacagacaa ttatcggtca
    42721 gggcgatcac ctcgtctgct gcgcgattaa cactttctgg tgaatccaga tcgatcaaca
    42781 cgccggtaaa tcccatgttg ttcatacgct caacatcatt cggttttcgg caacctgcca
    42841 gcacatgaaa accctggcgt tttaattcga gcgcgctttc caggccaatt ccactggaac
    42901 atccggtaat taagaccgat ttttgcataa ctttacctgt caggatctcc gttgctttat
    42961 gagtcatgat ttactaaagg ctgcaactgc ttcgccatcc agtcggcaat aaacggctgg
    43021 gcgtcgcggt tgggatgaat accgtcatcc tgcatccatt gtggcttgag gtagacctct
    43081 tccataaaaa agggcagcag cggaacatca aactctttgg cgagtttggg gtaaatggcg
    43141 ctaaaggctt cattataacg gcgaccatag tttgcaggca gacgtatttg cattaacaat
    43201 ggttcagcgt tggcggcttt gacatcctgc aaaatctggc gcagcgtttg ctcggtttgc
    43261 tgtggctgaa aaccacgcaa accatcattg cgcccagttc aaccagcacc caacgcggct
    43321 gatgctgttt gagcagagcc ggaaggcgcg ccagcccttg ttgcgaggtg tcgccgctga
    43381 tgctggcatt aactaccgac gttttactct gccacttatc attcaacaag gcaggccagg
    43441 ccgcgctggc agacattcga tacccggcgc tcaggctatc acccagaatc aataacgtgt
    43501 ccgctgcggc ggcacggaag gttaacagga ccaggaacag gaagggcaaa tgccagcgga
    43561 aaacattgtt gaagttcatc atcttaagaa gtccgtcggt cagggggagc atgaactctc
    43621 catcctcacc ggagttgagc tggttgtcaa acgtggcgag accatcgcac tggtgggcga
    43681 gtcgggatcg ggtaagtcaa ccttgctggc gatcctcgcc gggcttgatg acggcagcag
    43741 tggcgaagtg agtctggtgg gacaaccgct acataatatg gacgaagaag cgcgggcaaa
    43801 gttgcgcgcg aagcacgtcg gctttgtttt tcagtcattt atgttaattc ctacccttaa
    43861 cgcgctggaa aacgtcgagc ttccggctct gctgcgcggt gagagtagcg cggaaagtcg
    43921 taacggggcg aaagcgttgc tcgaacagtt agggctgggt aaacgtctgg atcatcttcc
    43981 ggcacagctt tccggcggtg aacagcaacg agtggcgctg gcacgagcct ttaatggtcg
    44041 acctgatgtg ctgtttgccg acgaacccac cggcaacctt gaccgccaga cgggcgataa
    44101 aattgccgac ctgctgtttt ccctcaaccg tgaacatggc accacgttga ttatggtgac
    44161 ccacgacctg caactggcgg cacgctgcga ccgctgctta cggctggtga acgggcagtt
    44221 gcaggaggaa gcatgattgc acgttggttc tggcgcgaat ggcgttcgcc gtcgctatta
    44281 attgtctggc tggcgctaag cctggcggtg gcctgcgtgc tggcgctggg caatatcagc
    44341 gatcgcatgg agaagggctt aagccagcaa agccgtgagt ttatggcggg cgatcgggcg
    44401 ttgcgcagtt cacgcgaagt gccgcaagcg tggctggagg aagcgcaaaa gcgcggcctg
    44461 aaagtcggca agcagctgac tttcgccaca atgacctttg caggcgacac accgcagctg
    44521 gcgaacgtca aagcggtgga tgatatctac ccgatgtatg gcggtctgca aactaatccc
    44581 cctggcctga aaccgcaggc gggcagcgta ttgctggccc cacgcctgat ggcactgctt
    44641 aacctgaaaa cgggcgacac cattgacgtg ggcgatgcca ccttgcggat tgccggagaa
    44701 gtgattcagg aaccggattc cggttttaac cccttccaga tggctccgcg tctgatgatg
    44761 aatctggcgg atgtcgataa aaccggagcc gtgcagccgg ggagtcgggt cacctggcgt
    44821 tataaattcg gcggcaacga gaaccagctc gacggctatg agaaatggtt gttacctcag
    44881 cttaaacccg aacaacgctg gtacggtctg gaacaggacg aaggcgcgct ggggcgatcg
    44941 atggaacgct cgcaacagtt cctgctgctt tcggcgcttc tgaccttgct gctggcagtg
    45001 gcagcggtgg cggtagcgat gaatcattac tgtcgcagtc gctacgatct ggtggcgatc
    45061 ctcaaaacgc tgggggcagg gcgagcgcaa ctgcgtaagc taatcgtcgg tcagtggttg
    45121 atggtgctga cgctttcagc cgttaccggt ggggccatag gcctgttgtt cgaaaacgtg
    45181 ttgatggtgc tgctcaagcc cgttctgcct gctgcactac cgccagccag cctctggccg
    45241 tggctgtggg cgcttggcac catgacggtc atctcgctgc tggtggggct acgaccatac
    45301 cgcttgttgc tggcaacgca gcctttacgc gtattacgta atgatgtggt cgcgaacgtc
    45361 tggccgctga agttttatct gccgattgtc agtgtggtgg ttgtgctgct gctcgccgga
    45421 ttaatgggtg gcagcatgct gctttgggcg gtgctggcgg gcgcggtagt actggctttg
    45481 ctgtgcggtg tgctgggctg gatgctgctg aatgtacttc gccgcatgac gctgaaatcg
    45541 ctgcctctgc gcctggcggt tagccgcctg ttacgtcagc cgtggtcaac gttaagccag
    45601 ctttcggcat tttcgctctc ctttatgctg ctggcactgc tgctggtgct gcgtggcgat
    45661 ctgctcgacc gctggcagca gcagctaccg ccagaaagcc cgaactactt tttaattaac
    45721 atcgccacag aacaggtagc accgctaaaa gcgttcctcg cggaacatca tatagtcccg
    45781 gaatcatttt atccggtagt gcgggcgcgg ctgacggcga ttaatgataa gccgacagaa
    45841 ggtaatgaag atgaggcgct taatcgcgaa ctcaatctta cctggcaaaa tacgcggccc
    45901 gatcataatc cgattgtcgc cggtaactgg ccgccaaaag ccgatgaagt gtcgatggaa
    45961 gaggggctgg cgaaacgctt aaacgttgcc ctcggtgata ccgtgacttt tatgggcgat
    46021 acccaggagt tccgcgctaa agtgaccagc ctgcgcaaag tggactggga aagtctgcgg
    46081 cctaatttct attttatttt ccctgaaggg gcattagacg ggcaaccgca gagctggctt
    46141 accagtttcc gctgggagaa tggcaacggc atgttgacgc aactcaaccg ccagttcccg
    46201 accattagcc tgttagacat tggcgcgatt ttaaaacagg tcggtcaggt gctggagcag
    46261 gtaagtcggg cgctggaggt gatggtggtg ctggtcaccg cctgcggtat gttgctgttg
    46321 ctggcacagg tgcaggtggg aatgcgccag cgtcatcagg agctggtggt atggcgcaca
    46381 ctcggtgcgg ggaaaaaact gctgcgcacg acattgtggt gtgatgttcg ccatgcttgg
    46441 gtttgtttcc ggcctggtgg ccgcaattgg tgcggaaacg gcactggcag tgttgcaggc
    46501 gaaagtgttt gatttcccgt gggagccaga ctggcgattg tggattgttc tgccgtgcag
    46561 cggtgcgctg ctgctgtcgc ttttcggcgg ctggctgggt gcgcgactgg ttaagggtaa
    46621 ggcgctgttc aggcagtttg tggggtgatg acaggtgtta gattgcctga tgcgctacgc
    46681 ttatcaggcc tacgaagagc gtgcaatata ttgattttgc aagattgtgt aggccggata
    46741 aggcgtttac gccgcatctg gcatgaacag tgtgcatttt ggcaataaat atgaaaccaa
    46801 ctgtcatgtc ggttttttta aaggccgaag gaggtaaacc ttcggcctta gctacgtttc
    46861 ctcagttatt caataaactg acggatcagt tccagcagcg cgattagcgc cttaagaacg
    46921 atgagaactt tatcgagaaa agttttcatc cgttgctccc ttaagggaga aagacaatca
    46981 cagcttaccc ttacgttgcc tataactatg attttcttca tgtgctagat gaggactgtc
    47041 tctctccaaa ttagagcacc agaatcagca ccacctgtat cctgaccagg acttaaaagt
    47101 acttttccgc actgcggtaa tcccttgagg cagtatcact actagcgtcg ggattgtatc
    47161 gccaacatag ctgtttgaat atgtaggcat cctgacttat aattcacttg ctagatgagg
    47221 actgtttaat ttctgcccgg aaccaccaag tcttgggcgg aaaaaataaa aaaaccgact
    47281 gtcatgtcgg tttttttata tctgtattct ggtgcaacca ttttgttctc acgacaatcg
    47341 ccgtttcaga ataccgccca tcactgcgcc ttaacccata ccgccacttc cgcccactca
    47401 ccgcgaaaga caactttttc cgcttttttc tcaagctgat agcgatacat tgggtcgtaa
    47461 tattctttaa gtaacggcac cagccaggcc agatgaccgt cggtgctgcc ggtggtgagt
    47521 tgcgttgtca gcgccgtatc cagctgggca gcgagttcgt tatagcgctg taaccccagc
    47581 cgacgcttaa tcgccgaaag tccgtgatgc aggtattcgc aatattcctg ccagccctgt
    47641 tcgtcgccat aagcgtgggt aaaatcatga tgcatacgca agaaatactc ttcattcagg
    47701 cgttcaagac ggatctcaaa cggatcttct accaccgcaa tcgccgcctg agtcattcgc
    47761 tcgcgcagac attccggcag gtgattcgaa ccgatcatcc ggctttcatc ttccagcacc
    47821 cacaggcgca aatcctgacg ggcgtcggtt tttagcattt cggcagccag caggttttca
    47881 aaactcgcct ggctaagttg tggttgtaac gtgcgaccaa acgccgaacc gcgatgacgc
    47941 gccagccctt ccagatcaac accgtttggc tgttgttgca ctaatagcgt tttaccgctg
    48001 ccggtacaac cgccaatcag cactatcggt ttttgtgcca gttcaatggt cgcctgaatc
    48061 gcggtctggc gcagtgcctt ataaccgcct tccaccagcg gataatcaat ccccgcttca
    48121 tgcagccagc gttgcacaat atgtgaacgt tgaccgccac gggcgcagca gagaatacct
    48181 tgcggatttt gcaggcacgc tgcccgccag gcgtacatac gctgctgacg aatttcaccc
    48241 gccaccagtt tatgccccag cgccagcgct gcgtctgagc cttgctgttt atagcaggtg
    48301 ccaacggagg cgcgttcatc gttatccatt aacggcagat tgatagcggc gggcattgcg
    48361 ccttgctcaa actcgatagg ggcgcgaaca tcaataatgg gcgtatcagc aatcagcagg
    48421 gcacgatagt cctgttccgt gtgtctctct tgcatagtta aaagtgaacc tcaaatcagc
    48481 ttgcgcgcta ttttacgcgc caacgcgcaa ggaaacttga tttttaactg cgtggggtgc
    48541 cgaaaatttc taaaaatccg ctgatttccg gcctgtgttg ggtaaacaag gtcacaatat
    48601 cttctaccgc tttgcagctg ccaaatttgc gccatgccag actcaatggc gaaggagggc
    48661 gcatcgttgg gattacccgg ctgaccagtt gttgattatc gatcattgac tggcaaagtg
    48721 attttggcaa aaaaccaatg ccaacacccg ccagatgggc ggcgattttc gtttccatat
    48781 cgggaacaat aatctctttt tgccccggca atcgccaggc gacgcgtttg gttaaggtgc
    48841 cggcgctgtc ttcaatattg accgccggaa agcgacgcaa ctgcgcttct gttagcggct
    48901 cttcaacgtt cgccagcgga tgatccgccg ccatgacaaa tcgccattgc accgatccta
    48961 agggatcaag actaaaggta tttgccagcg cctcagttcc cgtgaccccg atagccagcg
    49021 aaaaaccttc gtacaatagc gagtcccaga cgcccatata gatttgtcgg gagatgtgaa
    49081 actgggtaaa ggggtaacgc tcattcagcc acgccagcaa ctgggcgacg gcctgggggt
    49141 tatagagcag gttgttgatg acaatattca cctggcgttc cacgccatca ttcacctgtt
    49201 gcagctcgct gggcatactt tccagccagc tcagccagtc tctggcctgg gaaagtagat
    49261 gctcgccagc cgctgtcaac gttacgctac gagtcgtacg gaaaaacagc gctactccgg
    49321 tattctcttc cagaagttta atgcgataac tgatcgtcgc cgtggtttta cataatcgtt
    49381 ctgccgcttt tgaaaaactt cctgtttcag caaccgcaat gaaagtccgc aaggtttctg
    49441 gatcgaacat cttcaggtat ccccttttaa atccgcaagt tgcgtgattt tcttatcctc
    49501 tgatttatca gtatttttac atgataatcc tgttcaattt gtggactaaa tctagttttg
    49561 gaaaaacatt ccaacttttg tattaatgtt gttctcttaa ggttttagat tgcctgttat
    49621 tgaaaccaag ctgaccggtc ggcggtggtt gaacggaatt atgttacaag gacaaaaaga
    49681 tgaaacttca ggtattaccg ttaagtcagg aagcctttag tgcttatggc gacgtaatcg
    49741 aaacgcagca acgggatttt ttccatatta acaatggcct ggtggagcgt taccacgatt
    49801 tggcgctggt tgagattctt gagcaagacc gtacgcttat cagcattaac cgcgcgcaac
    49861 cggcgaatct gccgctgacc attcacgaac tcgaacgtca tccgctgggc attcaggcct
    49921 ttatcccaat gaaaggtgaa gtgtttgtgg tggtcgtggc gttaggcgac gacaaaccag
    49981 acctgtcaac gctgcgggcg tttatcacca acggtgaaca gggggtgaat taccatcgta
    50041 acgtctggca tcacccactt ttcgcctggc agcgcgtcac cgattttctg accatcgatc
    50101 gcggcggcag tgacaactgt gatgttgaaa gtattcctga acaggaactc tgttttgcgt
    50161 gacgcctgca accgacttgc ataagataaa ctaattgttc attgtttatg ctcacttgta
    50221 ggtcggagtt aacgtaggta tgacggaagt tagacggcgc ggcaggccag gacaggcgga
    50281 gcctgtggca cagaagggcg cacaggcgtt agagcgggga attgcgattc tgcaatatct
    50341 ggaaaaaagt gggggaagtt cgtcggttag cgatatttct ctcaatctgg atttgccgct
    50401 ctccacgacc tttcgcttgc tgaaggtttt acaagcagcg gattttgtct atcaggacag
    50461 tcaattaggc tggtggcata taggattagg tgtctttaac gtcggtgcgg cgtacatcca
    50521 taaccgcgat gtcctctccg tcgccgggcc gtttatgcgc cgcctgatgt tactttccgg
    50581 cgaaacggtc aatgtcgcga tccgtaacgg caatgaagcg gtattaattg gtcagttaga
    50641 gtgtaaatcg atggtcagga tgtgtgcgcc actgggcagt cgtctgccgc tgcatgcttc
    50701 cggtgcgggc aaagcgctgc tttatccgct ggcggaagag gagttgatga gcatcattct
    50761 gcaaaccggt ttgcagcagt ttacgccaac tacgcttgtg gatatgccca ccttgctgaa
    50821 ggacctggaa caagcgcgtg aactgggcta taccgtagat aaagaagagc atgttgtagg
    50881 tctgaattgc atagcttcag caatttacga tgatgtcggt agagttgttg ccgctatctc
    50941 catctccggg ccttcatcaa gactgacaga agatcgtttt gtcagtcagg gtgagctggt
    51001 cagagacacc gcccgcgata tcagcacggc gttgggactg aaagcacatc catagtgtct
    51061 gtcgcatccc gctctgcgga gcgggttttt ttgacaaaat ttgaaagttg gaaaattttt
    51121 ccaactaata gaggtaggaa taaaatggca aaaatgagag ccgttgacgc ggcaatgtat
    51181 gtgctggaga aagaaggtat caccaccgcc ttcggcgttc cgggagctgc aattaatccg
    51241 ttctactcag cgatgcgtaa gcacggcggt attcgtcaca ttctggcgcg tcatgtggaa
    51301 ggtgtttcgc acatggcgga aggttatacc cgcgcaacgg cagggaatat cggcgtatgt
    51361 ctggggactt ccggtcctgc gggcacggac atgatcaccg cgctctattc cgcttctgct
    51421 gattccattc ctattctgtg cattaccggc caggcaccgc gcgcccgtct gcataaagaa
    51481 gattttcagg ccgtagatat tgaagcaatt gctaaaccgg tcagcaaaat ggcggttaca
    51541 gttcgtgaag cggcgctggt gcctcgcgtg ctgcaacagg catttcacct gatgcgttct
    51601 ggtcgtccgg gtccggtact ggtggattta ccgttcgacg ttcaggttgc ggaaatcgag
    51661 tttgatcctg acatgtacga accgctgccg gtctacaaac ctgctgccag ccgtatgcag
    51721 atcgaaaaag ctgtagaaat gttaatccag gccgaacgtc cggtgattgt tgccgggggc
    51781 ggggtaatca atgctgacgc agctgcactg ttacaacagt ttgctgaact gaccagcgtt
    51841 ccggtgatcc caacgctgat gggctggggc tgtatcccgg acgatcatga actgatggcc
    51901 gggatggtcg gtctgcaaac cgcgcatcgt tacggtaacg caacgttgct ggcgtccgac
    51961 atggtgtttg gtatcggtaa ccgttttgct aaccgtcata ccggttcggt agagaaatac
    52021 accgaagggc gcaaaatcgt tcatattgat attgagccga cgcaaattgg tcgcgtgctg
    52081 tgtccggatc tcggcattgt ctctgatgct aaagcggcgc tgacactgct ggttgaagtg
    52141 gcgcaggaga tgcaaaaagc gggtcgtctg ccgtgtcgta aagaatgggt cgccgactgc
    52201 cagcagcgta aacgcacttt gctgcgcaaa acccacttcg acaacgtgcc ggtgaaaccg
    52261 cagcgcgtgt atgaagagat gaacaaagcc tttggtcgcg atgtttgtta tgtcaccact
    52321 attggtctgt cacaaatcgc tgcggcacaa atgctgcatg tctttaaaga ccgccactgg
    52381 atcaactgtg gtcaggctgg tccgttaggc tggacgattc cggcggcgct ggggggttgt
    52441 gccgctgatc cgaaacgcaa tgtggtggcg atttctggcg actttgactt ccagttcctg
    52501 attgaagagt tagccgttgg cgcgcagttc aacattccgt acatccatgt gctggtgaat
    52561 aacgcctatc tcggcctgat tcgtcagtca caacgcgctt ttgacatgga ctactgcgtg
    52621 caactcgctt tcgagaatat caactccagt gaagtgaatg gctacggtgt tgaccacgta
    52681 aaagtagcgg aaggtttagg ttgtaaagct attcgggtct tcaaaccgga agatattgcg
    52741 ccagcctttg aacaggcgaa agccttaatg gcgcaatatc gggtaccggt agtcgtggaa
    52801 gttattctcg agcgtgtgac caatatttcg atgggcagcg aactggataa cgtcatggaa
    52861 tttgaagata tcgccgataa cgcagcggat gcaccgactg aaacctgctt catgcactat
    52921 gaataaggga gataaatgat gttacgtttc tctgctaatt tatcgatgtt atttggtgaa
    52981 tatgattttc tcgcccgttt tgagaaagct gcgcagtgtg gttttcgcgg cgttgaattt
    53041 atgtttcctt atgactacga cattgaagaa ttaaaacagg tactggcgag taataaactc
    53101 gaacatacgc tgcacaattt accggcaggt gactgggcgg cgggcgagcg cggtattgcc
    53161 tgtattcctg gccgtgaaga agagtttcgg gatggcgtag cagcagcgat t
//
LOCUS       DAJTRC010000017        52597 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.017, whole genome shotgun sequence.
ACCESSION   DAJTRC010000017 DAJTRC010000000
VERSION     DAJTRC010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 52597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 52597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52597
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.017"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..354
                     /locus_tag="OO357_001094"
     CDS             <1..354
                     /locus_tag="OO357_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000871680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial family protein"
                     /protein_id="HCR5886116.1"
                     /translation="YQFHHIRFDCIQGEQGELVFEPSDVEYYFEPVTIQESGTSILKN
                     ELENSEDGAGQIGFQLSNDGTNEIQYGKSNYYSFQHPHEGSNQIPLFIRPRTYGNNVS
                     SGQIMSRVKIVVMYN"
     gene            complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="OO357_001095"
     CDS             complement(392..1291)
                     /gene="nhaR"
                     /locus_tag="OO357_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705976.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional activator NhaR"
                     /protein_id="HCR5886117.1"
                     /translation="MSHINYNHLYYFWHVYKEGSVVGAAEALYLTPQTITGQIRALEE
                     RLQGKLFKRKGRGLEPSELGELVYRYADKMFTLSQEMLDIVNYRKESNLLFDVGVADA
                     LSKRLVSSVLNAAVVEGEPIHLRCFESTHEMLLEQLSQHKLDMIISDCPIDSTQQEGL
                     FSVRIGECGVSFWCTNPPPEKPFPACLEERRLLIPGRRSMLGRKLLNWFNSQGLNVEI
                     LGEFDDAALMKAFGAMHNAIFVAPTLYAYDFYADKTVVEIGRVENVMEEYHAIFAERM
                     IQHPAVQRICNTDYSALFSPAVR"
     gene            complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="OO357_001096"
     CDS             complement(1357..2523)
                     /gene="nhaA"
                     /locus_tag="OO357_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HCR5886118.1"
                     /translation="MKHLHRFFSSDASGGIILIIAAILAMIMANSGATSGWYHDFLET
                     PVQLRVGSLEINKNMLLWINDALMAVFFLLVGLEVKRELMQGSLASLRQAAFPVIAAI
                     GGMIVPALLYLAFNYADPITREGWAIPAATDIAFALGVLALLGSRVPLVLKIFLMALA
                     IIDDLGAIIIIALFYTNDLSMASLGVAAVAIAVLAVLNLCGVRRTGVYILVGVVLWTA
                     VLKSGVHATLAGVIVGFFIPLKEKHGRSPAKRLEHVLHPWVAYLILPLFAFANAGVSL
                     QGVTLDGLTSILPLGIIAGLLIGKPLGISLFCWLALRLKLAHLPEGTTYQQIMVVGIL
                     CGIGFTMSIFIASLAFGSVDPELINWAKLGILVGSISSAVIGYSWLRVRLRPSV"
     gene            3109..3261
                     /gene="mokC"
                     /locus_tag="OO357_001097"
     CDS             3109..3261
                     /gene="mokC"
                     /locus_tag="OO357_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin MokC"
                     /protein_id="HCR5886119.1"
                     /translation="MKQHKAMIVALIVICITAVVAALVTRKDLCEVHIRTGQTEVAVF
                     TAYESE"
     gene            complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="OO357_001098"
     CDS             complement(3365..4495)
                     /gene="dnaJ"
                     /locus_tag="OO357_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HCR5886120.1"
                     /translation="MAKQDYYEILGVSKTAEEREIRKAYKRLAMKYHPDRNQGDKEAE
                     AKFKEIKEAYEVLTDSQKRAAYDQYGHAAFEQGGMGGGGFGGGADFSDIFGDVFGDIF
                     GGGRGRQRAARGADLRYNMELTLEEAVRGVTKEIRIPTLEECDVCHGSGAKPGTQPQT
                     CPTCHGSGQVQMRQGFFAVQQTCPHCQGRGTLIKDPCNKCHGHGRVERSKTLSVKIPA
                     GVDTGDRIRLAGEGEAGEHGAPAGDLYVQVQVKQHPIFEREGNNLYCEVPINFAMAAL
                     GGEIEVPTLDGRVKLKVPGETQTGKLFRMRGKGVKSVRGGAQGDLLCRVVVETPVGLN
                     EKQKQLLQELQESFGGPTGEHNSPRSKSFFDGVKKFFDDLTR"
     gene            complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="OO357_001099"
     CDS             complement(4584..6500)
                     /gene="dnaK"
                     /locus_tag="OO357_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001737966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HCR5886121.1"
                     /translation="MGKIIGIDLGTTNSCVAIMDGTIPRVLENAEGDRTTPSIIAYTQ
                     DGETLVGQPAKRQAVTNPQNTLFAIKRLIGRRFQDEEVQRDVSIMPFKIIAADNGDAW
                     VEVKGQKMAPPQISAEVLKKMKKTAEDYLGEPVTEAVITVPAYFNDAQRQATKDAGRI
                     AGLEVKRIINEPTAAALAYGLDKGTGNRTIAVYDLGGGTFDISIIEIDEVDGEKTFEV
                     LATNGDTHLGGEDFDSRLINYLVEEFKKDQGIDLRNDPLAMQRLKEAAEKAKIELSSA
                     QQTDVNLPYITADATGPKHMNIKVTRAKLESLVEDLVNRSIEPLKVALQDAGLSVSDI
                     DDVILVGGQTRMPMVQKKVAEFFGKEPRKDVNPDEAVAIGAAVQGGVLTGDVKDVLLL
                     DVTPLSLGIETMGGVMTTLIAKNTTIPTKHSQVFSTAEDNQSAVTIHVLQGERKRAAD
                     NKSLGQFNLDGINPAPRGMPQIEVTFDIDADGILHVSAKDKNSGKEQKITIKASSGLN
                     EDEIQKMVRDAEANAEADRKFEELVQTRNQGDHLLHSTRKQVEEAGDKLPADDKTAIE
                     SALTALETALKGEDKAAIEAKMQELAQVSQKLMEIAQQQHAQQQTAGADASANNAKDD
                     DVVDAEFEEVKDKK"
     gene            6877..7281
                     /locus_tag="OO357_001100"
     CDS             6877..7281
                     /locus_tag="OO357_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2541 family protein"
                     /protein_id="HCR5886122.1"
                     /translation="MKSVFTISASLAISLMLCCTAQANDHKLLGVIAMPRNETNDLAL
                     KLPVCRIVKRIQLSADHGDLQLSGASVYFKAARSASQSLNIPSEIKEGQTTDWININS
                     DNDNKRCVSKITFSGHTVNSSDMATLKIIGDD"
     gene            7307..8020
                     /gene="msyB"
                     /locus_tag="OO357_001101"
     CDS             7307..8020
                     /gene="msyB"
                     /locus_tag="OO357_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acidic protein MsyB"
                     /protein_id="HCR5886123.1"
                     /translation="MNVNYLNDSDLDFLQHCSEEQLANFARLLTHNEKGKTRLSSILM
                     RNELFKSMEGHPEQHRRNWQLIAGELQHFGGDSIANKLRGHGKLYRAILLDVSKRLKL
                     KADKEMSTFEIEQQLLEQFLRNTWKKMDEEHKQEFLHAVDARVNELEELLPLLMKDKL
                     LAKGVSHLLSSQLTRILRTHAAMSVLGHGLLRGAGLGGPVGAALNGVKAVSGSTYRVT
                     IPAVLQIACLRRMVSATQV"
     gene            8169..8735
                     /gene="satP"
                     /locus_tag="OO357_001102"
     CDS             8169..8735
                     /gene="satP"
                     /locus_tag="OO357_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate uptake transporter"
                     /protein_id="HCR5886124.1"
                     /translation="MGNTKLANPAPLGLMGFGMTTILLNLHNVGYFALDGIILAMGIF
                     YGGIAQIFAGLLEYKKGNTFGLTAFTSYGSFWLTLVAILLMPKLGLTDAPNAQFLGVY
                     LGLWGVFTLFMFFGTLKGARVLQFVFFSLTVLFALLAIGNIAGNAAIIHFAGWIGLIC
                     GASAIYLAMGEVLNEQFGRTVLPIGESH"
     gene            complement(8770..9357)
                     /gene="mog"
                     /locus_tag="OO357_001103"
     CDS             complement(8770..9357)
                     /gene="mog"
                     /locus_tag="OO357_001103"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HCR5886125.1"
                     /translation="MNTLRIGLVSISDRASSGVYQDKGIPALEEWLTSALTTPFELET
                     RLIPDEQAIIEQTLCELVDEMSCHLVLTTGGTGPARRDVTPDATLAVADREMPGFGEQ
                     MRQISLHFVPTAILSRQVGVIRKQALILNLPGQPKSIKETLEGVKDAEGNVVVHGIFA
                     SVPYCIQLLEGPYVETAPEVVAAFRPKSARRDVSE"
     gene            complement(9472..10425)
                     /gene="tal"
                     /locus_tag="OO357_001104"
     CDS             complement(9472..10425)
                     /gene="tal"
                     /locus_tag="OO357_001104"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HCR5886126.1"
                     /translation="MTDKLTSLRQYTTVVADTGDIAAMKLYQPQDATTNPSLILNAAQ
                     IPEYRKLIDDAVAWAKQQSNDRAQQIVDATDKLAVNIGLEILKLVPGRISTEVDARLS
                     YDTEASIAKAKRLIKLYNDAGISNDRILIKLASTWQGIRAAEQLEKEGINCNLTLLFS
                     FAQARACAEAGVFLISPFVGRILDWYKANTDKKEYAPAEDPGVVSVSEIYQYYKEHGY
                     ETVVMGASFRNIGEILELAGCDRLTIAPALLKELAESEGAIERKLSYTGEVKARPARI
                     TESEFLWQHNQDPMAVDKLAEGIRKFAIDQEKLEKMIGDLL"
     gene            10704..12134
                     /locus_tag="OO357_001105"
                     /pseudo
     CDS             10704..12134
                     /locus_tag="OO357_001105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308034.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:alanine symporter family protein"
     gene            12204..12980
                     /gene="yaaA"
                     /locus_tag="OO357_001106"
     CDS             12204..12980
                     /gene="yaaA"
                     /locus_tag="OO357_001106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide stress protein YaaA"
                     /protein_id="HCR5886127.1"
                     /translation="MLILISPAKTLDYQSPLTTTRYTLPELLDNSQQLIHEARKLTPP
                     QISTLMRISDKLAGINAARFHDWQPDFTPANARQAILAFKGDVYTGLQAETFSEDDFD
                     FAQQHLRMLSGLYGVLRPLDLMQPYRLEMGIRLENARGKDLYQFWGDIITNKLNEALA
                     AQGDNVVINLASDEYFKSVKPKKLNAEIIKPVFLDEKNGKFKIISFYAKKARGLMSRF
                     IIENRLTKPEQLTGFNSEGYFFDEDSSSNGELVFKRYEQR"
     gene            complement(13133..13429)
                     /locus_tag="OO357_001107"
     CDS             complement(13133..13429)
                     /locus_tag="OO357_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2502 domain-containing protein"
                     /protein_id="HCR5886128.1"
                     /translation="MKKMQSIVLALSLVLVAPMAAQAAEITLVPSVKLQIGDRDNRGY
                     YWDGGHWRDHGWWKQHYEWRGNRWHPHGPPPPPRHHKKAPHDHHGGHGPGKHHR"
     gene            complement(13643..14929)
                     /gene="thrC"
                     /locus_tag="OO357_001108"
     CDS             complement(13643..14929)
                     /gene="thrC"
                     /locus_tag="OO357_001108"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HCR5886129.1"
                     /translation="MKLYNLKDHNEQVSFAQAVTQGLGKNQGLFFPHDLPEFSLTEID
                     EMLKLDFVTRSAKILSAFIGDEIPQEILEERVRAAFAFPAPVANVESDVGCLELFHGP
                     TLAFKDFGGRFMAQMLTHIADDKPVTILTATSGDTGAAVAHAFYGLPNVKVVILYPRG
                     KISPLQEKLFCTLGGNIETVAIDGDFDACQALVKQAFDDEELKVALGLNSANSINISR
                     LLAQICYYFEAVAQLPQEARNQLVVSVPSGNFGDLTAGLLAKSFGLPVKRFIAATNVN
                     DTVPRFLHDGQWSPKATQATLSNAMDVSQPNNWPRVEELFRRKIWQLKELGYAAVDDE
                     TTQQTMRELKELGYTSEPHAAVAYRALRDQLNPGEYGLFLGTAHPAKFKESVEAILGE
                     TLDLPKELAERADLPLLSHNLPADFSALRKLMMNHQ"
     gene            complement(14930..15862)
                     /gene="thrB"
                     /locus_tag="OO357_001109"
     CDS             complement(14930..15862)
                     /gene="thrB"
                     /locus_tag="OO357_001109"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_705963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HCR5886130.1"
                     /translation="MVKVYAPASSANMSVGFDVLGAAVAPVDGALLGDVVTVEAAETF
                     SLNKLGRFADKLPSEPRENIVYQCWERFCQELGKQIPVAMTLEKNMPIGSGLGSSACS
                     VVAALMAMNEHCGKPLNDTRLLALMGELEGRISGSIHYDNVAPCFLGGMQLMIEENDI
                     ISQQVPGFDEWLWVLAYPGIKVSTAEARAILPAQYRRQDCIAHGRHLAGFIHACYSRQ
                     PELAAKLMKDVIAEPYRERLLPGFRQARQAVAEIGAVASGISGSGPTLFALCDKPDTA
                     QRVADWLGKNYLQNQEGFVHICRLDTAGARVLEN"
     gene            complement(15864..18326)
                     /gene="thrA"
                     /locus_tag="OO357_001110"
     CDS             complement(15864..18326)
                     /gene="thrA"
                     /locus_tag="OO357_001110"
                     /EC_number="1.1.1.3"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005124053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aspartate kinase/homoserine
                     dehydrogenase I"
                     /protein_id="HCR5886131.1"
                     /translation="MRVLKFGGTSVANAERFLRVADILESNARQGQVATVLSAPAKIT
                     NHLVAMIEKTISGQDALPNISDAERIFAELLTGLAAAQPGFPLAQLKTFVDQEFAQIK
                     HVLHGISLLGQCPDSINAALICRGEKMSIAIMAGVLEARGHNVTVIDPVEKLLAVGHY
                     LESTVDIAESTRRIAASRIPADHMVLMAGFTAGNEKGELVVLGRNGSDYSAAVLAACL
                     RADCCEIWTDVDGVYTCDPRQVPDARLLKSMSYQEAMELSYFGAKVLHPRTITPIAQF
                     QIPCLIKNTGNPQAPGTLIGASRDEDELPVKGISNLNNMAMFSVSGPGMKGMVGMAAR
                     VFAAMSRARISVVLITQSSSEYSISFCVPQSDCVRAERAMQEEFYLELKEGLLEPLAV
                     TERLAIISVVGDGMRTLRGISAKFFAALARANINIVAIAQGSSERSISVVVNNDDATT
                     GVRVTHQMLFNTDQVIEVFVIGVGGVGGALLEQLKRQQSWLKNKHIDLRVCGVANSKA
                     LLTSVHGLNLENWQEELAQAKEPFNLGRLIRLVKEYHLLNPVIVDCTSSQAVADQYAD
                     FLREGFHVVTPNKKANTSSMDYYHQLRYAAEKSRRKFLYDTNVGAGLPVIENLQNLLN
                     AGDELVKFSGILSGSLSYIFGKLDEGMSFSEATTLAREMGYTEPDPRDDLSGMDVARK
                     LLILARETGRELELADIEIEPVLPAEFNAEGDVAAFMANLSQLDDLFAARVAKARDEG
                     KVLRYVGNIDEDGVCRVKIAEVDGNDPLFKVKNGENALAFYSHYYQPLPLVLRGYGAG
                     NDVTAAGVFADLLRTLSWKLGV"
     misc_feature    complement(18352..18471)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00506"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Thr leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00506"
     gene            complement(18407..18472)
                     /gene="thrL"
                     /locus_tag="OO357_001111"
     CDS             complement(18407..18472)
                     /gene="thrL"
                     /locus_tag="OO357_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thr operon leader peptide"
                     /protein_id="HCR5886132.1"
                     /translation="MKRISTTITTTITITTGNGAG"
     gene            complement(18686..19372)
                     /locus_tag="OO357_001112"
     CDS             complement(18686..19372)
                     /locus_tag="OO357_001112"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5886133.1"
                     /translation="MRITIILVAPARAENIGAAARAMKTMGFSELRIVDSQAHLEPAT
                     RWVAHGSGDIIDNIKVFPTLAESLHDVDFTVATTARSRAKYHYYATPVELVPLLEEKS
                     SWMSHAALVFGREDSGLTNEELALADVLTGVPMVADYPSLNLGQAVMVYCYQLATLIQ
                     QPAKSDTTADQHQLQALRERVMALLTTLAVADDIKLVDWLQQRLGLLEQRDTAMLHRL
                     LHDIEKNITK"
     gene            complement(19772..19912)
                     /locus_tag="OO357_001113"
     CDS             complement(19772..19912)
                     /locus_tag="OO357_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886134.1"
                     /translation="MTKVRNCVLDALSINVNNIISLVVGTFPQDPTVSKTAVILTILT
                     AT"
     gene            20008..20724
                     /gene="arcA"
                     /locus_tag="OO357_001114"
     CDS             20008..20724
                     /gene="arcA"
                     /locus_tag="OO357_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003621071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator ArcA"
                     /protein_id="HCR5886135.1"
                     /translation="MQTPHILIVEDELVTRNTLKSIFEAEGYDVFEATDGAEMHQILS
                     EYDINLVIMDINLPGKNGLLLARELREQANVALMFLTGRDNEVDKILGLEIGADDYIT
                     KPFNPRELTIRARNLLSRTMNLGTVSEERRSVESYKFNGWELDINSRSLIGPDGEQYK
                     LPRSEFRAMLHFCENPGKIQSRAELLKKMTGRELKPHDRTVDVTIRRIRKHFESTPDT
                     PEIIATIHGEGYRFCGDLED"
     gene            complement(20783..22135)
                     /gene="creD"
                     /locus_tag="OO357_001115"
     CDS             complement(20783..22135)
                     /gene="creD"
                     /locus_tag="OO357_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell envelope integrity protein CreD"
                     /protein_id="HCR5886136.1"
                     /translation="MLKSPLFWKMTTLFGAVLLLLIPIMLIRQVIVERADYRSDVEDA
                     IRQSTSGPQKLVGPLIAIPVTELYTVQEEDKTVERKRSFIHFWLPESLMVDGNQNVEE
                     RKIGIYTGQVWHSDLTLKADFDVSRLSELNAPNIILGKPFIVISVGDARGIGVVKAPE
                     VNGTALTIEPGTGLEQGGQGVHIPLPEGDWRKQNLQLNMALNLSGTGDLSVVPAGRNS
                     EMTLTSNWPHPSFLGDFLPAKREASESGFQAQWQSSWFANNLGERFASGNDTGWENFP
                     AFSVAVTTPADQYQLTDRATKYAILLIALTFMAFFVFETLTAQRLHPMQYLLVGLSLV
                     MFYLLLLALSEHIGFTVAWIIASLIGALMNGIYLQAVLKGWRNSMLFTLALLLLDGVM
                     WGLLNSADSALLLGTSVLVVALAGMMFVTRNIDWYAFSLPKMKASKEVTTDDELRIWK
                     "
     gene            complement(22193..23617)
                     /gene="creC"
                     /locus_tag="OO357_001116"
     CDS             complement(22193..23617)
                     /gene="creC"
                     /locus_tag="OO357_001116"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     CreC"
                     /protein_id="HCR5886137.1"
                     /translation="MRIGMRLLLGYFLLVAVAAWFVLAIFVKEVKPGVRRATEGTLID
                     TATLLAELARPDLLSGDPTHGQLAQAFNQLQHRPFRANIGGINKVRNEYHVYMTDAHG
                     KVLFDSANKAVGQDYSRWNDVWLTLRGQYGARSTLQNPADPESSVMYVAAPIMGGSRL
                     IGVLSVGKPNAAMAPVIKRSERRILWASAILLGIALVIGAGMVWWINRSIARLTRYAD
                     SVTDNKPVPLPDLGSSELRKLAQALESMRVKLEGKNYIEQYVYALTHELKSPLAAIRG
                     AAEILREGPPPEVVARFTDNILTQNARMQALVETLLRQARLENRQEVVLTAVDVAALF
                     RRVSEARTVQLAEKNITLHVMPTEVNVAAEPALLDQALGNLLDNAIDFTPESGCITLS
                     AEVDQEHVTLKVLDTGSGIPDYALSRIFERFYSLPRANGQKSSGLGLAFVSEVARLFN
                     GEVTLRNVQEGGVLASLRLHRHFT"
     gene            complement(23617..24306)
                     /gene="creB"
                     /locus_tag="OO357_001117"
     CDS             complement(23617..24306)
                     /gene="creB"
                     /locus_tag="OO357_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator CreB"
                     /protein_id="HCR5886138.1"
                     /translation="MQRETVWLVEDEQGIADTLVYMLQQEGFAVEVFERGLPVLDKAR
                     QQVPDVMILDVGLPDISGFELCRQLLALYPALPVLFLTARSEEVDRLLGLEIGADDYV
                     AKPFSPREVCARVRTLLRRVKKFSTPSPVIRIGHFELNEPAAQISWFDTPLTLTRYEF
                     LLLKTLLKSPGRVWSRQQLMDSVWEDAQDTYDRTVDIHIKTLRAKLRAINPDLSPINT
                     HRGMGYSLRGL"
     gene            complement(24319..24792)
                     /gene="creA"
                     /locus_tag="OO357_001118"
     CDS             complement(24319..24792)
                     /gene="creA"
                     /locus_tag="OO357_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein CreA"
                     /protein_id="HCR5886139.1"
                     /translation="MKYKHLILSLSLIMLGPLAHAEEIGSVDTVFKMIGPDHKIVVEA
                     FDDPDVKNVTCYVSRAKTGGIKGGLGLAEDTSDAAISCQQVGPIELSDRIKNGKAQGE
                     VVFKKRTSLVFKSLQVVRFYDAKRNALAYLAYSDKVVEGSPKNAISAVPVMPWRQ"
     gene            25003..25872
                     /gene="robA"
                     /locus_tag="OO357_001119"
     CDS             25003..25872
                     /gene="robA"
                     /locus_tag="OO357_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     RobA"
                     /protein_id="HCR5886140.1"
                     /translation="MDQAGIIRDLLIWLEGHLDQPLSLDNVAAKAGYSKWHLQRMFKD
                     VTGHAIGAYIRARRLSKSAVALRLTARPILDIALQYRFDSQQTFTRAFKKQFAQTPAL
                     YRRSPEWSAFGIRPPLRLGEFTMPEHKFVTLEDTPLIGVTQSYSCSLEQISDFRHEMR
                     YQFWHDFLGNAPTIPPVLYGLNETRPSQDKDDEQEVFYTTALAQDQADGYVLTGHPVM
                     LQGGEYVMFTYEGLGTGVQEFILTVYGTCMPMLNLTRRKGQDIERYYPAEDAKAGDRP
                     INLRCELLIPIRR"
     gene            complement(25869..26516)
                     /gene="gpmB"
                     /locus_tag="OO357_001120"
     CDS             complement(25869..26516)
                     /gene="gpmB"
                     /locus_tag="OO357_001120"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase GpmB"
                     /protein_id="HCR5886141.1"
                     /translation="MLQVYLVRHGETQWNAERRIQGQSDSPLTAKGEQQAMQVATRAK
                     ELGITHIISSDLGRTRRTAEIIAQACGCDIIFDSRLRELNMGVLEKRHIDSLTEEEEN
                     WRRQLVNGTVDGRIPEGESMQELSDRVNAALESCRDLPQGSRPLLVSHGIALGCLVST
                     ILGLPAWAERRLRLRNCSISRVDYQESLWLASGWVVETAGDISHLDAPALDELQR"
     gene            26568..27088
                     /gene="yjjX"
                     /locus_tag="OO357_001121"
                     /pseudo
     CDS             26568..27088
                     /gene="yjjX"
                     /locus_tag="OO357_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001649724.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
     gene            complement(27173..27499)
                     /gene="trpR"
                     /locus_tag="OO357_001122"
     CDS             complement(27173..27499)
                     /gene="trpR"
                     /locus_tag="OO357_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trp operon repressor"
                     /protein_id="HCR5886142.1"
                     /translation="MAQQSPYSAAMAEQRHQEWLRFVDLLKNAYQNDLHLPLLNLMLT
                     PDEREALGTRVRIVEELLRGEMSQRELKNELGAGIATITRGSNSLKAAPVELRQWLEE
                     VLLKSD"
     gene            complement(27589..29526)
                     /gene="sltY"
                     /locus_tag="OO357_001123"
     CDS             complement(27589..29526)
                     /gene="sltY"
                     /locus_tag="OO357_001123"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase"
                     /protein_id="HCR5886143.1"
                     /translation="MEKAKQVTWRLLAAGVCLLTVSSVARADSLDEQRSRYAQIKQAW
                     DNRQMDVVEQMMPGLKDYPLYPYLEYRQITDDLMNQPAVTVTNFVRANPTLPPARTLQ
                     SRFVNELARREDWRGLLAFSPEKPGTTEAQCNYYYAKWNTGQSEEAWQGAKELWLTGK
                     SQPNACDKLFSVWRASGKQDPLAYLERIRLAMKAGNTGLVTVLAGQMPADYQTIASAI
                     ISLANNPNTVLTFARTTGATDFTRQMAAVAFASVARQDAENARLMIPSLAQAQQLNED
                     QIQELRDIVAWRLMGNDVTDEQAKWRDDAIMRSQSTSLIERRVRMALGTGDRRGLNTW
                     LARLPMEAKEKDEWRYWQADLLLERGREAEAKEILHQLIQQRGFYPMVAAQRIGEEYE
                     LKIDKAPQNVDSTLTQGPEMARVRELMYWNLDNTARSEWANLVKSKSKTEQAQLARYA
                     FNNQWWDLSVQATIAGKLWDHLEERFPLAYNDLFKRYTSGKEIPQSYAMAIARQESAW
                     NPKVKSPVGASGLMQIMPGTATHTVKMFSIPGYSSPGQLLDPETNINIGTSYLQYVYQ
                     QFGNNRIFSSAAYNAGPGRVRTWLGNSAGRIDAVAFVESIPFSETRGYVKNVLAYDAY
                     YRYFMGDKPTLMSATEWGRRY"
     gene            29737..31404
                     /gene="ettA"
                     /locus_tag="OO357_001124"
     CDS             29737..31404
                     /gene="ettA"
                     /locus_tag="OO357_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017145039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-dependent translational throttle protein
                     EttA"
                     /protein_id="HCR5886144.1"
                     /translation="MAQFVYTMHRVGKVVPPKRHILKNISLSFFPGAKIGVLGLNGAG
                     KSTLLRIMAGIDKDIEGEARPQPDIKIGYLPQEPQLNPEHTVRESIEEAVSEVVNALK
                     RLDEVYALYADPDADFDKLAAEQGRLEEIIQAHDGHNLNVQLERAADALRLPDWDAKI
                     ANLSGGERRRVALCRLLLEKPDMLLLDEPTNHLDAESVAWLERFLHDFEGTVVAITHD
                     RYFLDNVAGWILELDRGEGIPWEGNYSSWLEQKDQRLAQEASQEAARRKSIEKELEWV
                     RQGTKGRQSKGKARLARFEELNSTEYQKRNETNELFIPPGPRLGDKVLEVSNLRKSYG
                     DRLLIDDLSFSIPKGAIVGIIGPNGAGKSTLFRMISGQEPPDSGTITLGETVKLASVD
                     QFRDSMDNSKTVWEEVSGGLDIMKIGNTEMPSRAYVGRFNFKGVDQGKRVGELSGGER
                     GRLHLAKLLQVGGNMLLLDEPTNDLDIETLRALENALLEFPGCAMVISHDRWFLDRIA
                     THILDYQDEGKVEFFEGNFTEYEEYKKRTLGADALEPKRIKYKRIAK"
     gene            complement(31511..31678)
                     /locus_tag="OO357_001125"
     CDS             complement(31511..31678)
                     /locus_tag="OO357_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886145.1"
                     /translation="MSFFDELKTSLEEAVEIKQGLKKPARVTRHEIEDAKAVVDRKRC
                     SRRIRHSVLNA"
     gene            complement(31712..32944)
                     /gene="nadR"
                     /locus_tag="OO357_001126"
     CDS             complement(31712..32944)
                     /gene="nadR"
                     /locus_tag="OO357_001126"
                     /EC_number="2.7.1.22"
                     /EC_number="2.7.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_463436.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multifunctional transcriptional
                     regulator/nicotinamide-nucleotide
                     adenylyltransferase/ribosylnicotinamide kinase NadR"
                     /protein_id="HCR5886146.1"
                     /translation="MSSFDYLKTAIKQQGCTLQQVADASGMTKGYLSQLLNAKIKSPS
                     AQKLEALHRFLGLEFPRQKKTIGVVFGKFYPLHTGHIYLIQRACSQVDELHIIMGFDD
                     TRDRALFEDSAMSQQPTVPDRLRWLLQTFKYQKNIRIHAFNEEGMEPYPHGWDVWSNG
                     IKKFMAEKGIQPDLIYTSEEADAPQYMEHLGIETVLVDPKRTFMSISGAQIRENPFRY
                     WEYIPTEVKPFFVRTVAILGGESSGKSTLVNKLANIFNTTSAWEYGRDYVFSHLGGDE
                     IALQYSDYDKIALGHAQYIDFAVKYANKVAFIDTDFVTTQAFCKKYEGREHPFVQALI
                     DEYRFDLVILLENNTPWVADGLRSLGSSVDRKEFQNLLVEMLEENNIEFVRVEEDDYD
                     SRFLRCVELVREMMGEQG"
     gene            complement(32965..34347)
                     /gene="radA"
                     /locus_tag="OO357_001127"
     CDS             complement(32965..34347)
                     /gene="radA"
                     /locus_tag="OO357_001127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001399683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HCR5886147.1"
                     /translation="MAKAPKRAFVCNECGADYPRWQGQCSACHAWNTITEVRLAASPT
                     VARNERLSGYAGSAGVAKVQKLSDISLEELPRFSTGFKEFDRVLGGGVVPGSAILIGG
                     NPGAGKSTLLLQTLCKLAQQMKTLYVTGEESLQQVAMRAHRLGLPTDNLNMLSETSIE
                     QICLIAEEEQPKLMVIDSIQVMHMADVQSSPGSVAQVRETAAYLTRFAKTRGVAIVMV
                     GHVTKDGSLAGPKVLEHCIDCSVLLDGDADSRFRTLRSHKNRFGAVNELGVFAMTEQG
                     LREVSNPSAIFLSRGDEVTSGSSVMVVWEGTRPLLVEIQALVDHSIMANPRRVAVGLE
                     QNRLAILLAVLHRHGGLQMADQDVFVNVVGGVKVTETSADLALLLAMVSSLRDRPLPQ
                     DLVVFGEVGLAGEIRPVPSGQERISEAAKHGFRRAIVPAANVPKKAPEGMQIFGVKKL
                     SDALSVFDDL"
     gene            complement(34396..35364)
                     /gene="serB"
                     /locus_tag="OO357_001128"
     CDS             complement(34396..35364)
                     /gene="serB"
                     /locus_tag="OO357_001128"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase"
                     /protein_id="HCR5886148.1"
                     /translation="MPNITWCDLPEDVSLWPGLPLSLSGDEVMPLDYHAGRSGWLLYG
                     RGLDKQRLTQYQSKLGAAMVIVAAWCVEDYQVIRLAGSLTARATRLAHEAQLDVAPLG
                     KIPHLRTPGLLVMDMDSTAIQIECIDEIAKLAGTGEMVAEVTERAMRGELDFTASLRS
                     RVATLKGADANILQQVRENLPLMPGLTQLVLKLETLGWKVAIASGGFTFFAEYLRDKL
                     RLTAVVANELEIMDGKFTGNVIGDIVDAQYKAKTLTRLAQEYEIPLAQTVAIGDGAND
                     LPMIKAAGLGIAYHAKPKVNEKAEVTIRHADLMGVFCILSGSLNQK"
     gene            35470..36114
                     /locus_tag="OO357_001129"
     CDS             35470..36114
                     /locus_tag="OO357_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtjB family periplasmic protein"
                     /protein_id="HCR5886149.1"
                     /translation="MARTKLKFRLHRAVIVLFCLALLVALMQGASWFSQNHQRQRNPQ
                     LEELARTLARQVTLNVAPLMRTDSPDEKRIQAILDQLTDESRILDAGVYDEQGDLIAR
                     SGESVEVRDRLALDGKKAGGYFNQQIVEPIAGKNGPLGYLRLTLDTHTLATEAQQVDN
                     TTNILRLMLLLSLAIGVVLTRTLLQGKRTRWQQSPFLLTASKPVPEEEESEKKE"
     gene            36142..37157
                     /gene="lplA"
                     /locus_tag="OO357_001130"
                     /pseudo
     CDS             36142..37157
                     /gene="lplA"
                     /locus_tag="OO357_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418803.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase LplA"
     gene            37189..37452
                     /locus_tag="OO357_001131"
     CDS             37189..37452
                     /locus_tag="OO357_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5886150.1"
                     /translation="MIDLENQEREIINLMFSQRISWLAAVRIRHKLSLAEVSKMLGIS
                     INSLKQIEKTERLSSNIKSKMAEIYGCPPELLICPSWMTAEHK"
     gene            complement(37613..38332)
                     /gene="deoD"
                     /locus_tag="OO357_001132"
     CDS             complement(37613..38332)
                     /gene="deoD"
                     /locus_tag="OO357_001132"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HCR5886151.1"
                     /translation="MATPHINAEMGDFADVVLMPGDPLRAKYIAETFLEDAREVNNVR
                     GMLGFTGTYKGRKISVMGHGMGIPSCSIYTKELITDFGVKKIIRVGSCGAVLPHVKLR
                     DVVIGMGACTDSKVNRIRFKDHDFAAIADFDMVRNAVDAAKALGVDARVGNLFSADLF
                     YSPDGEMFDVMEKYGILGVEMEAAGIYGVAAEFGAKALTICTVSDHIRTHEQTTAAER
                     QTTFNDMIKIALESVLLGDKE"
     gene            complement(38389..39612)
                     /gene="deoB"
                     /locus_tag="OO357_001133"
     CDS             complement(38389..39612)
                     /gene="deoB"
                     /locus_tag="OO357_001133"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HCR5886152.1"
                     /translation="MKRAFIMVLDSFGIGATEDAERFGDVGADTLGHIAEACAKGEAD
                     NGRKGPLNLPNLTRLGLAKAHEGSTGFIPAGMDGNAEVIGAYAWAHEMSSGKDTPSGH
                     WEIAGVPVLFEWGYFSDHENSFPQELLDKLVERANLPGYLGNCHSSGTVILDQLGEEH
                     MKTGKPIFYTSADSVFQIACHEETFGLDKLYELCEIAREELTNGGYNIGRVIARPFIG
                     DKAGNFQRTGNRHDLAVEPPAPTVLQKLVDEKHGQVVSVGKIADIYANCGITKKVKAT
                     GLDALFDATIKEMKEAGDNTIVFTNFVDFDSSWGHRRDVAGYAAGLELFDRRLPELMS
                     LLRDDDILILTADHGCDPTWTGTDHTREHIPVLVYGPKVKPGSLGHRETFADIGQTLA
                     KYFGTSDMEYGKAMF"
     gene            complement(39664..40986)
                     /gene="deoA"
                     /locus_tag="OO357_001134"
     CDS             complement(39664..40986)
                     /gene="deoA"
                     /locus_tag="OO357_001134"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HCR5886153.1"
                     /translation="MFLAQEIIRKKRDGHALSDEEIRFFINGIRDNTISEGQIAALAM
                     TIFFHDMTMPERVSLTMAMRDSGTVLDWKSLHLNGPIVDKHSTGGVGDVTSLMLGPMV
                     AACGGYIPMISGRGLGHTGGTLDKLESIPGFDIFPDDNRFREIIKDVGVAIIGQTSSL
                     APADKRFYATRDITATVDSIPLITASILAKKLAEGLDALVMDVKVGSGAFMPTYELSE
                     ALAEAIVGVANGAGVRTTALLTDMNQVLASSAGNAVEVREAVQFLTGEYRNPRLFDVT
                     MALCVEMLISGKLAKDDAEARAKLQAVLDNGKAAEVFGRMVAAQKGPTDFVENYAKYL
                     PTAMLTKAVYADTEGFVSEMDTRALGMAVVAMGGGRRQASDTIDYSVGFTDMARLGDQ
                     VDGQRPLAVILAKDENSWQEAAKAVKAAIKLADNAPESTPTVYRRISE"
     gene            complement(41113..41892)
                     /gene="deoC"
                     /locus_tag="OO357_001135"
     CDS             complement(41113..41892)
                     /gene="deoC"
                     /locus_tag="OO357_001135"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005097947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HCR5886154.1"
                     /translation="MTDLKASSLRALKLMDLTTLNDDDTDEKVIALCHQAKTPVGNTA
                     AICIYPRFIPIARKTLKEQGTPEIRIATVTNFPHGNDDIEIALAETRAAIAYGADEVD
                     VVFPYRALMAGDEQVGFDLVKACKEACAAANVLLKVIIETGELKDEALIRKASEISIK
                     AGADFIKTSTGKVAVNATPESARIMMEVIRDMGVEKTVGFKPAGGVRTAEDAQKYLAI
                     ADELFGADWADARHYRFGASSLLASLLKALGHGDGKSASSY"
     gene            42150..43691
                     /locus_tag="OO357_001136"
     CDS             42150..43691
                     /locus_tag="OO357_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjI family glycine radical enzyme"
                     /protein_id="HCR5886155.1"
                     /translation="MPTSHENALQQRCQQIVTSPVLSPEQKRHFLALEAENNLPYPQL
                     PAEARRALDEGVICDMFEGHAPYKPRYVLPDYARFLANGSEWLELEGAKDLDDALSLL
                     TILYHHVPSVTSMPVYLGQLDALLQPYVRILTQNEIDVRIKRFWRYLDRTLPDAFMHA
                     NIGPSDSPITRAILRADAQVSPNLTFIYDPEITPDDLLLEVAKNICECSKPHIANGPV
                     HDKIFTKGGYGIVSCYNSLPLAGGGSTLVRLNLKAIAERSESLDDFFTRTLPHYCQQQ
                     IAIIDARCEFLYQQSHFFENSFLVKEGLINPERFVPMFGMYGLAEAVNLLCEKEGIAA
                     RYGKEAAANEVGYRISAQLAEFVANTPVKYGWQKRAMLHAQSGISSDIGTTPGARLPY
                     GDEPDPITHLQTVAPHHAYYYSGISDILTLDETIKRNPQALVQLCLGAFKAGMREFTA
                     NVSGNDLVRVTGYMVRLSDLEKYRAEGSRTNTTWLGEEAARNTRILERQPRVISHEQQ
                     MRFSQ"
     gene            43663..44526
                     /locus_tag="OO357_001137"
     CDS             43663..44526
                     /locus_tag="OO357_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjW family glycine radical enzyme activase"
                     /protein_id="HCR5886156.1"
                     /translation="MNSRCALVSKIIPFSCVDGPGSRLALFLQGCNLRCKNCHNPWTM
                     GRCNDCGECVPQCPHQALQIVDGKVVWNAVVCEQCDTCLKMCPQHATPMAQSMSVDEV
                     LSHVRKAVLFIEGITVSGGEATTQLPFVVALFTAIKNDPQLRHLTCLVDSNGMLSETG
                     WEKLLPVCDGAMLDLKAWGSECHQQLTGRDNQQIKRSIYLLAERGKLAELRLLMIPGQ
                     VDYLQHIEELAAFIKGLGDVPVRLNAFHAHGVYGEAQSWASATPEDVEPLADALKVRG
                     VSRLIFPALYL"
     gene            complement(44740..45522)
                     /locus_tag="OO357_001138"
     CDS             complement(44740..45522)
                     /locus_tag="OO357_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5886157.1"
                     /translation="MICRFIDTHCHFDFPPFIGDEEASLQRAAQAGVGKIIVPATEAE
                     NFARVQALAENYQPLYAVLGLHPGMLEKHSDVSLEQLQQALERRPAKVVAVGEIGLDL
                     FGDDPQFERQQWLLDEQLKLAKRYDLPVILHSRRTHDKLAMHLKRHDLPRAGVVHGFS
                     GSQQQAERFVQLGYKIGVGGTITYPRASKTRDVIAKLPLASLLLETDAPDMPLNGFQG
                     QPNRPEQAARVFAVLCELRPEPADEIAEVLLNNTYAVFNVRG"
     gene            complement(45519..46592)
                     /locus_tag="OO357_001139"
     CDS             complement(45519..46592)
                     /locus_tag="OO357_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin family protein"
                     /protein_id="HCR5886158.1"
                     /translation="MGQRIPVTLGNIAPLSLRPFQPGRIALVCEGGGQRGIFTAGVLD
                     EFMRAQFNPFDLYLGTSAGAQNLSAFICNQPGYARKVIMRYTTKREFFDPLRFVRGGN
                     LIDLDWLVEATASQMPLQMDTAARLFDSGKSFYMCACRQDDYAPNYFLPTKQNWLDVI
                     RASSAIPGFYRSGVSLEGINYLDGGISDAIPVKEAARQGAKTLVVIRTVPSQMYYTPQ
                     WFKRMERWLGDSSLQPLVNLVQHHETSYRDIQQFIEKPPGKLRISEIYPPKPLHSIAL
                     GSRIPALREDYKLGRLCGRYFLATVGKLLTEKAPLTRHLVPVVTPESIVIPPAPVAND
                     TLVAEVSDAPQANDPTFNNEDLA"
     gene            complement(46714..46875)
                     /locus_tag="OO357_001140"
     CDS             complement(46714..46875)
                     /locus_tag="OO357_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002887716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 domain-containing protein"
                     /protein_id="HCR5886159.1"
                     /translation="MFRWGIIFLVIALIAAALGFGGLAGTAAGAAKIVFVVGIILFLV
                     SLFMGRKRP"
     gene            complement(46992..47597)
                     /gene="osmY"
                     /locus_tag="OO357_001141"
     CDS             complement(46992..47597)
                     /gene="osmY"
                     /locus_tag="OO357_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone OsmY"
                     /protein_id="HCR5886160.1"
                     /translation="MTMTRLKISKTLLAVMLTSAVATGSAYAENNAQTTNESAGQKVD
                     SSMNKVGNFMDDSAITAKVKAALVDHDNIKSTDISVKTDQKVVTLSGFVESQAQAEEA
                     VKVAKGVEGVTSVSDKLHVRDAKEGSVKGYAGDTATTSEIKAKLLADDIVPSRKVKVE
                     TTDGVVQLSGTVDSQAQSDRAESIAKAVDGVKSVKNDLKTK"
     gene            complement(47990..49579)
                     /gene="prfC"
                     /locus_tag="OO357_001142"
     CDS             complement(47990..49579)
                     /gene="prfC"
                     /locus_tag="OO357_001142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HCR5886161.1"
                     /translation="MTLSPYLKEVAKRRTFAIISHPDAGKTTITEKVLLFGQAIQTAG
                     TVKGRGSNQHAKSDWMEMEKQRGISITTSVMQFPYHDCLVNLLDTPGHEDFSEDTYRT
                     LTAVDCCLMVIDAAKGVEDRTRKLMEVTRLRDTPILTFMNKLDRDIRDPMELLDEVEN
                     ELKIGCAPITWPIGCGKLFKGVYHLYKDETYLYQSGKGHTIQEVRIVKGLNNPDLDAA
                     VGEDLAQQLRDELELVKGASNEFDKELFLAGEITPVFFGTALGNFGVDHMLNGLVEWA
                     PAPMPRQTDTRTVEASEDKFTGFVFKIQANMDPKHRDRVAFMRVVSGKYEKGMKLRQV
                     RTAKDVVISDALTFMAGDRSHVEEAYPGDILGLHNHGTIQIGDTFTQGEMMKFTGIPN
                     FAPELFRRIRLKDPLKQKQLLKGLVQLSEEGAVQVFRPISNNDLIVGAVGVLQFDVVV
                     ARLKSEYNVEAVYESVNVATARWVECADAKKFEEFKRKNESQLALDGGDNLAYIATSM
                     VNLRLAQERYPDVQFHQTREH"
     gene            complement(49670..50347)
                     /gene="yjjG"
                     /locus_tag="OO357_001143"
     CDS             complement(49670..50347)
                     /gene="yjjG"
                     /locus_tag="OO357_001143"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HCR5886162.1"
                     /translation="MKWDWIFFDADETLFTFDSFTGLQRMFLDYSVTFTAEDFQDYQA
                     VNKPLWVDYQNGAITSLQLQHGRFESWAERLKVEAGLLNDAFINAMAEICTPLPGAVS
                     LLNAIRSNAKIGIITNGFSALQQVRLERTGLRDYFDLLVISEEVGVAKPNKKIFDYAL
                     EQAGNPDRSRVLMVGDTAESDILGGINAGLATCWLNAHNREQPEGIAPTWTVSSLHEL
                     EQLLCKH"
     gene            complement(50362..50808)
                     /gene="rimI"
                     /locus_tag="OO357_001144"
     CDS             complement(50362..50808)
                     /gene="rimI"
                     /locus_tag="OO357_001144"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HCR5886163.1"
                     /translation="MNTISSLETTDLPAAYHIEQRAHAFPWSEKTFASNQGERYLNFQ
                     LTQNGKMAAFAITQVVLDEATLFNIAVDPDYQRQGLGRALLEHLIDELEKRGVATLWL
                     EVRASNAAAIALYESLGFNEATIRRNYYPTTDGREDAIIMALPISM"
     gene            complement(50777..51190)
                     /gene="holD"
                     /locus_tag="OO357_001145"
     CDS             complement(50777..51190)
                     /gene="holD"
                     /locus_tag="OO357_001145"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710111.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit psi"
                     /protein_id="HCR5886164.1"
                     /translation="MTSRRDWQLQQLGITQWSLRRPGALQGEIAIASPAHVRLVMVAN
                     DLPALTDPLVSDVLRALTVSPDQVLQLTPEKIAMLPQGSRCNSWRLGTDEPLSLEGAQ
                     VASPALTELRANPTARAALWQQICTYEHDFFPRND"
     gene            51293..52324
                     /gene="rsmC"
                     /locus_tag="OO357_001146"
     CDS             51293..52324
                     /gene="rsmC"
                     /locus_tag="OO357_001146"
                     /EC_number="2.1.1.172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1207)-N(2))-methyltransferase
                     RsmC"
                     /protein_id="HCR5886165.1"
                     /translation="MSAFTPASEVLLRHSDDFEQSRILFAGDLQDDLPARLDTAASRA
                     HTQQFHHWQVLSCQMGDNARFSLVATANDVADCDTLIYYWPKNKPEAQFQLMNLLSLL
                     PVGTDIFVVGENRSGVRSAEQMLADYAPLNKVDSARRCGLYFGRLEKQPVFDADKFWG
                     EYSVDGLTVKTLPGVFSRDGLDVGSQLLLSTLTPHTKGKVLDVGCGAGVLSVAFARHS
                     PKIRLTLCDVSAPAVEASRATLAANGVEGEVFASNVFSEVKGCFDMIISNPPFHDGMQ
                     TSLDAAQTLIRGAVRHLNSGGELRIVANAFLPYPDVLDETFGFHEVIAQTGRFKVYRA
                     IMTRQAKKG"
BASE COUNT        12525 a        14235 c        13181 g        12656 t
ORIGIN      
        1 tatcagtttc atcatataag atttgactgc atccagggcg agcaaggtga attagttttt
       61 gaacccagtg acgttgaata ttactttgaa ccagtgacga tacaggagtc aggaaccagc
      121 atattaaaaa atgagttaga aaacagtgag gacggtgcgg gtcaaatagg ctttcaactc
      181 tccaatgatg gtacgaatga aatccagtat ggtaaaagca attactactc ttttcagcat
      241 ccccatgaag gaagtaatca gattcctcta tttattcgcc cgcgtacata tggaaacaat
      301 gtctcaagcg ggcaaataat gagccgagtc aaaatcgtgg taatgtacaa ctaatctatc
      361 tcccccacct taactttggg ggagctgccg attaacgcac cgctggacta aaaagcgcag
      421 aataatccgt attgcagatt cgctgtaccg ccgggtgctg aatcatccgc tcagcaaaaa
      481 tagcatggta ctcttccatc acattctcga cgcgaccaat ttctacgaca gttttatcgg
      541 cataaaagtc atatgcataa agcgttgggg caacgaagat tgcattgtgc atcgcaccaa
      601 aagctttcat caaagcggca tcatcaaact cgccgaggat ttctacgttt aatccctggg
      661 agttaaacca gttaagcaat ttgcgcccta acattgaacg tcgcccagga atcaaaagtc
      721 gccgttcttc cagacaagcc gggaacggtt tttctggtgg tggatttgta caccagaaac
      781 tcacgccaca ttcgccaatt ctcacggaga acaggccttc ctgctgcgta gagtctatcg
      841 gacagtcaga aatgatcata tccagtttat gctgacttaa ttgctccagc agcatttcgt
      901 gggtggattc gaagcagcga agatgaatgg gctcgccttc taccactgcg gcgttaagta
      961 cgctactgac caggcgtttg gaaagtgcat cagccacgcc aacgtcaaac aataaattgg
     1021 attctttgcg atagttcaca atatccagca tttcctggct taaggtgaac attttatcgg
     1081 catagcgata gaccagttct cccagctcgc tgggttcgag accacgtccc ttgcgtttaa
     1141 ataatttgcc ttgcaggcgc tcttccagcg ctcgaatctg tccggtaatg gtttgtggag
     1201 ttaaataaag cgcctccgct gcgccaacca cggaaccttc tttatagaca tgccagaagt
     1261 aatacaagtg gttgtaattg atatgagaca tgctcatttc tctccctgat aacaatgaaa
     1321 agggagccgt ttatggctcc ccggtaaacc gtcctgtcaa actgatggac gcaaacgaac
     1381 gcgtaaccag ctgtatccaa ttaccgccga agagatagaa ccgaccagga taccgagttt
     1441 cgcccagtta atcagttctg gatctacgct accaaaggcc aggctggcaa taaagataga
     1501 catagtaaaa ccgataccgc acaggatccc caccaccata atttgctgat aagtcgttcc
     1561 ctcaggcaga tgcgccagtt tcaaacgcag cgccaaccag cagaacagac taatccccag
     1621 cggtttgcca atcagcaagc cagcgatgat ccccaatggc agaatggagg tcaagccatc
     1681 cagcgtgacg ccttgcagtg aaacgccagc attagcaaat gcaaacagcg gcaaaatcag
     1741 atacgccacc cacgggtgca acacatgctc cagtcgcttc gctggagaac gcccatgctt
     1801 ctctttcaaa ggaataaaga agccgacaat tacccccgcc agagttgcgt gaacccccga
     1861 tttcaacacc gcagtccaca acaccacgcc aacaagaata tagacgcccg tgcggcgtac
     1921 accacacaga ttcaataccg cgagtaccgc aattgctaca gccgcgacgc caagggaggc
     1981 catcgataag tcattagtgt agaacaatgc gatgataatg atggccccaa gatcgtcgat
     2041 aatagccaga gccatcaaaa agatcttcag cactaacgga acacgacttc ccaacagcgc
     2101 cagtacacca agtgcaaaag caatgtcagt agccgccggg atcgcccacc cttcgcgggt
     2161 aatcggatcg gcatagttaa aagccagata gagtaatgcc ggcacaatca tcccaccaat
     2221 agcggcgata actggaaatg cggcctggcg taagctggct agcgatcctt gcatcagttc
     2281 acgtttaact tccagaccga ctaacaggaa aaataccgcc atcagcgcat catttatcca
     2341 taacagcatg tttttgttga tttcgagtga accaacccgg agctgaaccg gcgtctccag
     2401 aaagtcgtga taccatccac tggttgcgcc gctgttggcc ataatcatcg ccaggatagc
     2461 ggcaatgata agaataatgc ctcccgaggc atcactgcta aagaatcgat gcagatgttt
     2521 cactttttat ttctctttca ggtgaataga tcgtttttac tattttaccc cgcacgaatc
     2581 atcgttaaaa tcagattata gtgtaggaat aattcgtttt ttacgagcca ttgaagaatt
     2641 tgcgccatca gcggtgagat gcgtttttga cggctgtaag cgctcacgag tgaccctaag
     2701 cccagataat catagcttaa ggtgaggatt cctgagggat taattaggaa aagtaataat
     2761 taatttgttg tcagtcggcg atgcttgtta ggccaatatt tcaaaaataa tccagcttgt
     2821 cgtcaataat tgcgatgaag agataaatta aagttaaata attaaatcat taaaaaatta
     2881 cgccacaaaa atgcgaggcg tcttcaggat tagaatcgat aaaacaaaat gggttaaaga
     2941 gagcgatata atagcggcgg gtgcttgagg ctgtctgtct caggcattag ctgaacggca
     3001 gatagagaaa agccccgagt gatattttac catcaacccg aggcctccta tatgctgaac
     3061 acatgtagag tgcctcttac tgaccgtaag gtcaaggaga agagagcaat gaagcagcat
     3121 aaggcgatga ttgtcgccct gatcgtcatc tgtatcaccg ccgtagtggc ggcgctggta
     3181 acgagaaaag acctctgtga ggttcacatc cgaactggcc agacggaggt tgctgttttc
     3241 acggcttacg aatccgagta agagcaacgg cggggagtga tccccgccac tttacaggtg
     3301 ctcgcatatc ttcaacgcac cctattttta cccaggcctg cccacgggca ggcttttggg
     3361 gaggttagcg ggtcaggtcg tcaaaaaact tcttcacacc atcaaagaag ctctttgagc
     3421 gcgggctgtt gtgctcgccg gttgggccac cgaagctttc ttgcagctct tgcagcagct
     3481 gtttctgctt ttcgttcagg cctaccggtg tttcgacgac aacgcggcac agcaaatcac
     3541 cctgtgcgcc accgcggaca gacttgacgc ctttaccgcg catacggaat agcttaccgg
     3601 tctgggtttc gccaggcact ttcagtttga cgcgaccatc aagggtcggt acttcgattt
     3661 cgccacccag cgccgccata gcgaagttga tcgggacttc gcaatacagg ttgttgcctt
     3721 cacgctcgaa aatcgggtgc tgtttaacct gaacctgaac gtacagatcg cctgccggtg
     3781 cgccatgctc gcccgcttca ccttcgcccg caagacggat gcggtctcca gtgtccaccc
     3841 ctgccgggat tttaacggac agcgttttgc tgcgctcaac acgaccatga ccatgacatt
     3901 tgttgcacgg atctttgatc agcgtaccgc ggccctgaca gtgtggacag gtctgctgta
     3961 cagcgaagaa tccctggcgc atctgcacct gaccagaacc atgacaggtc ggacaggtct
     4021 gcggctgtgt acctggtttt gcaccgctac cgtggcaaac gtcacactct tccagagtcg
     4081 gaatgcggat ctctttggtc acgccacgta cagcttcttc gagggtgagc tccatgttat
     4141 agcgtaaatc agcaccgcgc gccgcacgtt gacgaccacg tccgccgcca aaaatatcgc
     4201 cgaaaacgtc accaaaaata tcgctgaagt ctgcgccgcc gccaaaacca ccgccgccca
     4261 tgcctccttg ctcaaacgca gcatgaccat actgatcgta tgccgcacgt ttttgcgagt
     4321 cggtcagaac ttcataagct tccttgatct ctttaaattt cgcctcggcc tctttgtcac
     4381 cctggttacg gtccgggtgg tatttcatgg ccaggcgttt gtaggccttt ctgatttcac
     4441 gctcttccgc tgttttggaa acgcctaaaa tctcgtaata atcttgctta gccatctttt
     4501 ttaaattgcc cctagatgaa tgcacgggcg gagaggaaat tccccttcgc ccgtgtcagt
     4561 ataattaccc gtttataggg cgattatttt ttgtctttga cttcttcaaa ttcagcgtcg
     4621 acaacatcgt catctttcgc gttgtttgca gaagcatcag caccggcagt ctgctgctgg
     4681 gcatgttgct gctgggcgat ttccatcagt ttctgggaaa cctgtgccag ttcctgcatt
     4741 ttcgcttcga tagcggcttt gtcttcacct ttcagagcag tttccagtgc agtcagcgca
     4801 gactcgatag cagttttgtc gtcagccggc agtttgtcgc ctgcttcttc aacctgctta
     4861 cgggtgctgt gcagcagatg gtcgccctgg ttgcgagtct gtaccagctc ttcaaactta
     4921 cggtcagctt cggcgttagc ttctgcgtcg cgtaccattt tctggatttc atcttcgttc
     4981 agaccagaag aagccttgat ggtgatcttc tgctctttac cgctgttttt atctttcgcg
     5041 gaaacgtgca ggataccgtc agcatcgata tcgaaggtaa cttcgatctg cggcatgccg
     5101 cgcggtgccg ggttgatacc atctaggttg aactgaccca gagatttgtt atcagccgca
     5161 cgtttacgtt caccctgcag cacatggatg gttaccgcag actggttgtc ttcagcggta
     5221 gagaacacct ggctgtgctt ggtcgggata gtggtgtttt tcgcgatcag cgtcgtcatc
     5281 acaccgccca tggtttcgat acccagagac agcggggtaa cgtccagcag cagtacgtct
     5341 tttacgtcac cagtcagaac accaccctga acagcagcac cgattgctac agcttcgtcc
     5401 gggttaacgt ctttacgcgg ctctttacca aagaactcag caactttctt ctgaaccatt
     5461 ggcatacgag tctgaccacc aacgaggata acgtcgtcga tatcagatac ggacaggcca
     5521 gcatcctgca gtgcaacttt cagcggctca atggaacggt ttaccagatc ttcaaccagg
     5581 ctttccagtt tcgcacgagt cactttgatg ttcatgtgtt tcggaccggt cgcgtctgca
     5641 gtgatgtatg gcaggttaac gtcggtctgc tgagcggaag acagttcgat tttcgctttt
     5701 tctgccgctt ctttcaggcg ctgcattgcc agcggatcgt tgcgcaggtc aatgccctga
     5761 tctttcttga attcttcaac cagatagttg atcagacggc tgtcgaagtc ttcaccaccc
     5821 aggtgggtat caccgttggt tgccagaact tcgaaggttt tttcgccgtc aacttcgtcg
     5881 atttcgataa tagaaatatc gaaagtacca ccccccaggt cataaaccgc gatagtacgg
     5941 ttgccagtgc ctttgtccag accgtaagcc agcgcagctg cggtcggttc gttgatgata
     6001 cgttttactt ccagaccagc gatacggcct gcgtctttgg ttgcctgacg ctgagcatcg
     6061 ttaaagtatg ccggtacggt gataacagct tcagttaccg gttcacccag gtaatcttca
     6121 gcggttttct tcattttttt cagcacttca gcagaaatct gcggcggtgc cattttctgg
     6181 cctttaactt cgacccatgc gtcgccgtta tcagcagcaa taattttgaa cggcatgatg
     6241 gaaacatcac gctgtacttc ttcgtcctgg aagcggcggc caatcaggcg tttaatcgca
     6301 aacagggtgt tttgcgggtt cgtcactgcc tgacgtttag ccggctgacc aaccagagtt
     6361 tcaccatcct gggtataggc aatgatagaa ggcgtggtgc gatcgccttc ggcgttctcc
     6421 agcacgcgag gaatggtgcc atccataatc gctacacaag agttggtagt acccaggtcg
     6481 ataccaatta ttttacccat ctaaacgtct ccactatata ttcggtcatc atgtggttgt
     6541 gagtctgtaa taggggcgaa acgtctggtt tcaactgccc aatttcattt ttttgcagac
     6601 tcactcactg cggttgacta ctaaatgggg tcgtaaccca cgtcatcaag ggggagatgc
     6661 aaaaattttt tgtgcttttg cgcagaaatt tcgcgatatg gcaggacaag acgtaaaatt
     6721 gacagcaatt ttattcatat aaagaatgaa tcgcagccaa ggcgttcatc aaaaaattgt
     6781 aataaaaaga aaagattacg tgcctgaatc ttctctttat cagcagtaaa ctagtgggta
     6841 ttcatccccc tacctcttcc cactaagaga atccttatga aatccgtttt tacgatttcc
     6901 gccagcctgg cgattagcct gatgctgtgc tgcacggcgc aggcaaacga ccataaactc
     6961 ctcggcgtca ttgcaatgcc gcgtaacgaa accaacgatc tggcgctgaa acttcctgtt
     7021 tgtcgcattg tgaaacgcat acaactctct gccgaccatg gcgatttaca gttaagtggt
     7081 gcatcggttt atttcaaagc cgcccgtagc gccagtcaga gcctgaatat tccttcagaa
     7141 ataaaagaag ggcaaaccac tgactggatc aacattaaca gcgataacga caataaacgc
     7201 tgcgtctcaa aaatcacctt ttccggtcat acggtgaact catcggatat ggccacgctg
     7261 aaaattatcg gcgacgacta acgccagaat atagaagcca caaaaaatga atgttaatta
     7321 cctgaatgat tcagatctgg attttcttca gcattgtagt gaggaacagt tggcaaattt
     7381 cgcccgattg ctcacccata atgaaaaagg caaaactcgc ctctccagca tactgatgcg
     7441 caacgaactg tttaaatcga tggaagggca tcccgagcaa catcgccgca actggcagct
     7501 gattgccgga gaattacagc attttggtgg cgatagtatc gccaacaaac tgcgcggaca
     7561 cggtaaattg tatcgggcca ttttgctcga tgtttcaaag cgattgaagc tgaaagccga
     7621 caaagagatg tctacgtttg aaattgagca acagttactg gaacaatttc tgcgtaatac
     7681 ctggaagaaa atggacgagg aacataagca ggagtttctg cacgcggtcg atgccagggt
     7741 gaatgagctg gaagagctgc tgccgctgct gatgaaagac aaattattgg caaaaggtgt
     7801 gtcgcatttg ctttccagcc aactgacccg cattttacgc acccacgcag caatgagcgt
     7861 acttgggcat ggtttgctgc gcggcgcggg gctgggaggc cctgtgggcg cagcattaaa
     7921 tggggttaaa gcggtcagcg gcagcaccta tcgcgtgacg attccagccg tactgcaaat
     7981 cgcctgcctg cgccggatgg ttagcgccac tcaggtctga taccggaaac cggggaaaat
     8041 tttcattttc ccctcaaaag atcgtagaca ctgccccact ggctgattat tatgccgcgc
     8101 cctgaaaaca ctacagttat tcagggaaat tatttcacca ttcattcgat gatgattttt
     8161 gaggaattat gggcaacact aagttggcta atccggcacc gctgggcctg atgggcttcg
     8221 gcatgaccac cattctgctt aacctgcaca acgtgggtta tttcgctctg gacggtatta
     8281 ttcttgccat gggcattttc tacggcggca tcgcgcaaat ttttgctggt ctgctggagt
     8341 acaaaaaagg caacactttc ggtttaaccg cattcacctc ttacggttct ttctggctga
     8401 cgctggttgc gattctgctg atgccgaaac tgggtctgac cgatgcgcca aatgcacagt
     8461 tccttggtgt ctacctgggt ctgtggggcg tatttacgct gtttatgttc ttcggcactc
     8521 tgaaaggcgc gcgcgtcctg caattcgttt tctttagcct gaccgtgctg tttgccctgc
     8581 tggcgatcgg taacattgcc ggtaatgccg cgatcatcca ctttgccggc tggattggtc
     8641 tgatctgcgg tgccagcgca atctatctgg cgatgggtga agtgctgaac gagcagtttg
     8701 gtcgcaccgt tctgccgatt ggtgaatccc actaattgtc ttgagatccc cccgctcggg
     8761 gggatttttt tattcgctaa cgtcgcgtct tgcactcttc ggtctgaatg cagcaaccac
     8821 ttctggtgcc gtttcaacgt atggcccttc cagcaactga atgcagtacg gtacgctggc
     8881 aaaaatacca tgcaccacaa cgttaccctc agcgtccttc acaccttcca gcgtctcttt
     8941 aatagacttc ggctgaccgg gtaagttaag gatcagcgcc tgtttgcgaa tcacgcccac
     9001 ctgacgcgaa aggatcgcag ttggtacaaa atgcaggctg atctggcgca tctgttcacc
     9061 aaagccaggc atctcgcggt ccgctactgc cagcgtcgca tcgggcgtta cgtcacgacg
     9121 cgccgggcca gttccgcccg tggtgagcac cagatggcaa ctcatttcat ccaccagctc
     9181 acacaacgtt tgctcgatga tcgcctgctc atcggggatt aagcgggttt ccagttcaaa
     9241 cggcgtggtt agcgccgatg tcagccattc ttccagcgct ggaatgcctt tatcctgata
     9301 aacgccgctg gatgcgcgat cagagatgga aactaagcca atacgtaaag tattcatcaa
     9361 gatttttccg tttaaacaat ctcgttaaac agaatgatac actgcgaagg gagtgacaga
     9421 caggcttcag aagaggtagc gtgaccgact tcccggtcac gctaagaatg attacagcag
     9481 atcgccgatc attttttcca gtttttcctg gtcaatagca aacttacgga taccttccgc
     9541 cagtttatct actgccattg gatcctggtt gtgctgccac aggaactcgg actcagtgat
     9601 acgcgccgga cgcgctttca cttcgccggt gtaagacagt ttacgttcga tagccccttc
     9661 gctctccgcc agctctttca gcagtgccgg tgcgatggtc agacggtcac agcctgccag
     9721 ttccagaatt tcgccgatgt tacggaagct tgcgcccata accacggttt cataaccgtg
     9781 ctctttgtag tactggtaga tttcagatac agaaaccacg cccggatctt ctgccggagc
     9841 gtactctttc ttatcggtat tcgctttgta ccagtcaaga atacggccaa caaacggcga
     9901 gatcaggaac acgcccgcct ccgcacaagc acgagcctga gcgaaggaga acagcagggt
     9961 caggttacag ttgatacctt ctttttccag ctgttctgca gcacggatac cctgccaggt
    10021 agaagccagt ttgatcagaa tacgatcgtt gctaatacct gcatcgttgt agagtttgat
    10081 caggcgtttt gctttcgcaa ttgacgcttc agtgtcatag gaaagacgcg catcaacttc
    10141 agttgagata cggcccggaa ccagtttcag gatttccaga ccaatattta ctgccagttt
    10201 gtcggtcgcg tccacgatct gctgcgcgcg atcgttgctc tgctgtttcg cccaggcgac
    10261 agcatcatca atcaacttac ggtattccgg aatctgcgct gcgttaagaa tgagagaagg
    10321 gttggttgtg gcatcctgcg gttgatacag cttcattgcc gcgatgtccc cagtgtcggc
    10381 cactacggtg gtgtactgac gaagggaggt caatttgtcc gtcatgatag tatttctctt
    10441 taaacagctt gttaggggga tgtaaccggt ctgccctgat gatatcacga cgcaacgtca
    10501 gcgcaaccgc cgacaagacg gttatcgcca ggcgagactg tttcggattt ctgacactgc
    10561 gttgatatcg cccaccattt ttatacaaaa cctcatgtat gctacgcaga agttatcaag
    10621 tacctcgtag cgtatatact tcttaaacaa ttggtaacgt ttacacagga aagtcatcgc
    10681 gaccggcaat aagagggata tgcatgccag attttttctc ctttattaac agcgtccttt
    10741 ggggatcggt aatgatttac ctgctcttcg gcgcaggttg ttggttcact tttcgcaccg
    10801 gatttgtgca gtttcgctac atccgccagt ttggcaaaag tcttaaaaat agcattcatc
    10861 cacagccagg cggtttaacc tcatttcagt cattgtgtac cagtcttgcg gcgcgcgtgg
    10921 gtagcggcaa cctggccggc gttgcgctgg caattaccgc cggtggacca ggtgccgtct
    10981 tttggatgtg ggttgctgcg tttatcggca tggcgacctc gtttgccgaa tgttcccttg
    11041 cacaacttta taaagaacgt gacgtcaatg ggcagtttcg tggcggaccg gcatggtata
    11101 tggcgcgcgg gctggggatg cgctggatgg gcgttctgtt cgccgtcttt ttgctcatcg
    11161 cctacggcat aattttcagc ggagttcagg cgaacgccgt tgcccgagcc ctgagttttt
    11221 cttttgattt tcctccgctg gtgacgggca ttattctcgc tgtctttgct ctgctggcaa
    11281 tcactcgtgg tcttcatggc gtcgcccggc tcatgcaggg gtttgtcccg ttgatggcga
    11341 taatctgggt actgaccagc ctggtgattt gcgtaataaa tatcgggcaa cttccccacg
    11401 tcatttggtc tatttttgag agtgcttttg gctggcagga agcggcaggc ggcgcggcgg
    11461 gatatacctt aagccaggcg attactaacg gttttcagcg cagtatgttt tccaatgagg
    11521 tgggaatggg atcgacgcca aacgcggcag cggcagctgc atcctggcct ccgcatccgg
    11581 cagcacaagg gattgtccag atgattggca tttttatcga caccctggtc atctgtacgg
    11641 caagcgccat gctgatatta ctggcgggta acggcacaac ctacatgccg ctggaaggta
    11701 ttcagcttat ccagaaggcg atgcgggtgc taatgggttc ctagggtgct gaatttgtta
    11761 ccctcgtggt tattctgttt gccttcagct ccatcgttgc caactacatt tatgccgaaa
    11821 acaatctctt ctttttacgc ctgaacaacc ctaaagcgat ctggtgtttg cggatctgca
    11881 ccttcgcaac ggtcatcggc ggcaccttgc taagccttcc gctgatgtgg caactggcag
    11941 atatcataat ggcctgcatg gctattacca atttgaccgc cattttactg ctctcgcctg
    12001 tggttcatac cattgccagt gattatctac gccagcgtaa actcggcgtg cgcccggtgt
    12061 ttgatccgtt gcgttatccg gatatcggtc gccagctttc tccggacgcg tgggatgatg
    12121 tttcgcagga gtaatcacaa ctatcgatca actcattctc tttttttgct aaagtcggca
    12181 taaatttcct gcaaggactg gatatgctga ttcttatttc acctgcgaaa acgcttgatt
    12241 accaaagccc gttgaccacc acgcgctata cgctgccgga gctgttagac aattcccagc
    12301 agttgatcca tgaagcgcgg aaactgacgc ctccgcagat tagcacgctg atgcgcatca
    12361 gcgacaaact ggcgggtatc aacgccgctc gctttcatga ctggcagcca gatttcacgc
    12421 cggcgaatgc ccgccaggcg attctggcgt ttaaaggtga tgtctacacc ggcttgcagg
    12481 ccgaaacctt cagcgaagac gatttcgatt ttgcccaaca gcatttgcga atgctttccg
    12541 gcttgtatgg cgtactgcgc ccgctcgatt taatgcagcc ttatcgtctg gaaatgggga
    12601 tccgtcttga gaatgcccga gggaaagatc tgtatcaatt ctggggagat atcatcacca
    12661 acaagctgaa cgaggcgctc gcagcacaag gcgataatgt ggtgattaat ctggcgtcag
    12721 atgagtattt taaatcggtg aagccgaaga aattgaatgc cgagattatc aagccggtgt
    12781 tcctcgatga gaagaacggc aaatttaaga tcatcagctt ctacgctaag aaagcacgcg
    12841 gtctgatgag tcgtttcatt attgaaaatc ggctgaccaa accagagcaa ctgactggtt
    12901 ttaatagcga aggttacttc tttgatgaag attcctccag caatggcgaa ctggtgttta
    12961 aacgctacga gcagcgttaa gtttgtagtc aatgccggat gcggcgtgaa cgccttatcc
    13021 ggcctacaaa agcatgcaga ttcaatatat tgcagaatta acgtaggcct gataagacgc
    13081 gccagcgtcg catcaggcgc tgaatgccgg attgttaccc ggcatttgtc atttagcggt
    13141 gatgtttgcc tggaccatga ccgccgtgat gatcatgagg agctttctta tggtggcgcg
    13201 gcggtggcgg cggtccgtgt gggtgccagc gattgcctcg ccattcataa tgttgtttcc
    13261 accagccgtg gtcgcgccag tgacctccat cccagtaata gccacgatta tcacgatcgc
    13321 ctatctgtaa ttttactgac gggactaacg taatttccgc agcctgtgct gccatgggag
    13381 cgaccagaac cagggaaagt gcgagtacga tagattgcat ctttttcaca ggttactcct
    13441 tttatcattg cccgttatgg gtcgatatcc ggagattacg cgatgagaac gacggttgtt
    13501 attctccgca atcctaatct ctaagttacc gcatttattg ataatttctc ctttttccct
    13561 gtcgtttttc tccataattt cttcataaaa aagccgggct gcataaaagc aaacccggcc
    13621 tgattgagat aatgaataga ttttactgat gattcatcat caatttacgc aacgcagaaa
    13681 aatcggcggg cagattatgt gaaagcaagg gtaaatcagc acgttctgcc agctcttttg
    13741 gcagatccaa cgtttcaccg agaatcgctt ccacgctctc tttaaatttc gccggatgcg
    13801 cggtgccgag gaacaagcca tattcgcctg gattcaactg gtcacgcagc gcacgatacg
    13861 ctacggcagc gtgcggctcc gaggtgtagc ccagttcttt taactcacgc attgtctgtt
    13921 gcgtggtttc atcatccacg gctgcataac ccagctcttt cagttgccag attttgcggc
    13981 ggaacaactc ttccacacgc ggccagttgt tcggctgact cacgtccatc gcgttggata
    14041 acgtcgcctg agtcgctttg ggtgaccact gaccgtcgtg caggaaacgt ggcacggtat
    14101 cgttcacgtt ggtcgcagca ataaaacgtt tcaccggcag accgaatgac ttcgccagca
    14161 gacccgccgt caaatcgccg aagtttccgc ttggcaccga gacaaccagc tggttgcgcg
    14221 cctcctgcgg cagctgcgca acagcttcaa agtagtagca aatctgcgcc agcaaacggc
    14281 tgatgttaat cgagttagcc gagtttaacc ccagcgccac tttcagttct tcatcatcaa
    14341 acgcctgctt caccagcgcc tgacaggcat cgaaatcgcc gtcgatggca acagtttcga
    14401 tattgccgcc caacgtacag aacagttttt cttgcagtgg actgattttg cctcgtggat
    14461 agaggataac cactttcaca ttcggcaaac cgtagaaagc atgagccact gcagctccgg
    14521 tatcaccgga ggtcgcggtc agaatggtca ctggcttatc atccgcaata tgggtcagca
    14581 tttgtgccat aaagcgaccg ccgaaatctt taaatgccag cgttggcccg tggaacaatt
    14641 ccagacaacc gacatcgctt tcaacattgg cgaccggagc cgggaaggca aacgccgcgc
    14701 gcacgcgctc ttccaggatt tcctgtggga tttcatcacc aataaacgcc gagaggatct
    14761 tcgcactgcg ggtgacaaaa tccagcttca gcatctcatc aatttcagtc aggctgaatt
    14821 ccggcaggtc gtgcggaaaa aacagcccct gatttttgcc caacccctgg gttacggctt
    14881 gcgcaaagct gacctgctcg ttgtgatctt tcagattgta gagtttcatt tagttttcca
    14941 gtactcgtgc gcccgccgta tccagccggc aaatatgaac aaaaccttcc tgattttgca
    15001 ggtagttctt acccaaccag tcggcaacgc gctgggcggt atccggcttg tcacatagag
    15061 cgaacaaggt cgggccggag ccggagatac cgctcgctac cgcgccgatt tccgcgaccg
    15121 cctgccgcgc ctgccggaag ccaggcagta accgttcacg gtagggttca gcgataacat
    15181 ctttcatcag cttcgcggca agctcaggct gacgggaata gcaggcgtga atgaagccag
    15241 ccagatgtcg cccgtgcgca atgcaatcct ggcggcgata ctgcgccggt aaaatagccc
    15301 gggcttctgc cgtcgagact ttaattcccg gatacgccag cacccacagc cactcatcaa
    15361 accctggcac ttgctggctg atgatgtcgt tttcttcgat catcaactgc ataccaccaa
    15421 gaaaacacgg tgccacgttg tcgtaatgaa tgctgccgga gatacgtcct tccagctcgc
    15481 ccatcaaagc cagcaaacga gtgtcattaa gcggcttgcc gcagtgttca ttcatcgcca
    15541 tcagcgccgc gaccaccgaa caggcgctgg agcctaagcc cgaaccgatc ggcatattct
    15601 tttccagagt catcgccact ggaatttgct tgccaagctc ctggcaaaaa cgctcccagc
    15661 actgataaac gatattttcc cgtggttctg acggcagctt atcggcaaag cgtccgagtt
    15721 tgttgagact gaatgtctct gccgcctcaa ccgtgactac atctccgagc aatgcaccat
    15781 caacgggtgc caccgccgcc ccgagcacat caaacccgac gctcatattg gcactggaag
    15841 ccggggcata aactttaacc atgtcagact cctaacttcc atgagagggt acgtagcaga
    15901 tcggcaaaga caccggcagc ggtaacgtca ttgcccgcac catatccgcg cagcaccaac
    15961 ggcagcggct gataatagtg gctataaaag gccagggcgt tttcgccatt tttcactttg
    16021 aacagcggat cattcccatc cacttcggca atcttcacgc ggcagacacc atcttcatca
    16081 atattgccaa catagcgcaa aacttttcct tcatcacggg ccttcgccac gcgcgcggca
    16141 aagagatcgt cgagctgtga cagattcgcc ataaaagcgg caacatcacc ctcggcgtta
    16201 aactctgcgg gcagcacagg ttcaatttca atatccgcca gctccagttc acgtcccgtt
    16261 tcacgagcga gaatcaatag tttacgcgcc acatccatac cagaaagatc atctcgcggg
    16321 tccggttcgg tataacccat ttcccgcgcc agcgtggtcg cctcggagaa actcatgcct
    16381 tcgtctaact tgccgaagat ataagaaagc gaacctgaaa gaatgccgga gaacttcacc
    16441 aattcatcac ctgcattgag cagattttgc aggttctcaa taaccggtaa tccagcccca
    16501 acgttggtgt catagaggaa tttacgccgc gatttttccg ccgcataacg caactgatga
    16561 tagtaatcca tcgacgaggt gttggccttt ttgttcggcg tgacaacgtg gaaaccttcg
    16621 cgcaggaagt cggcatattg atccgccact gcctggctgg aggtgcagtc aacaatgacc
    16681 gggttcagca gatgatattc tttcacgagg cgaattaagc gcccgagatt aaacggctct
    16741 ttggcttgtg caagttcttc ctgccagttt tccagattaa ggccatgcac actagtgagc
    16801 agagccttcg agttggcaac gccgcagaca cgtaagtcga tatgtttatt cttcagccag
    16861 ctttgctgac gcttcagttg ctccagcagc gcaccgccaa cgccaccgac gccaatcaca
    16921 aacacttcga taacctgatc ggtattgaac agcatctgat gagtaacgcg cacgccagtg
    16981 gtcgcatcat cgttatttac cacgacagag attgagcgtt cagaagatcc ctgagcaatg
    17041 gcgacaatgt tgatattggc gcgggccagc gcggcaaaga atttcgccga gatcccacgc
    17101 aaggtgcgca taccatcacc taccaccgag ataatggcca gccgttccgt caccgccagc
    17161 ggctccagta agccttcttt cagttccagg tagaactctt cctgcattgc ccgttcagct
    17221 cgcacacagt cgctttgtgg aacgcagaaa ctgatactgt attcggaaga tgattgcgta
    17281 atcagcacca cggaaatacg ggcgcgtgac attgcagcaa agacgcgcgc cgccatgcca
    17341 accatccctt tcatccccgg gccggaaacg ctgaacattg ccatgttatt cagattggaa
    17401 atgcccttga ccggtaattc gtcttcatca cggctggcac caatgagcgt acctggtgct
    17461 tgaggatttc cggtattttt aatcaggcaa gggatctgga actgggcgat gggggtaatg
    17521 gtgcgggggt gaagaacttt agcgccgaag taggaaagct ccatcgcttc ctggtaggac
    17581 atcgacttca acaacctcgc atcgggcacc tgacgcgggt cgcaggtata gaccccgtca
    17641 acgtccgtcc aaatctcgca acaatcggcg cgtaaacagg cagccagcac cgccgcggag
    17701 tagtcggaac cgttacgtcc aagcaccacc agttcgcctt tttcattacc ggcggtgaaa
    17761 cctgccatca acaccatgtg atcagccgga atgcggcttg ccgcaatacg gcgggtggac
    17821 tcagcaatat cgacggtaga ttcgaggtaa tgccccactg ccagcagttt ttcgaccgga
    17881 tcgataacgg taacgttgtg accacgcgct tctaacacgc cggccataat ggcgatcgac
    17941 attttctcgc cacggcaaat cagcgcagcg ttgatgctat ccgggcactg ccccaacaaa
    18001 ctaatgccat gcaggacatg ttttatttgg gcaaattcct gatcgacgaa agttttcaat
    18061 tgcgccagcg ggaaccccgg ctgggcggcg gcgagtcccg tcaaaagttc ggcaaaaata
    18121 cgttcggcat cgctgatatt gggtaaagca tcctggccgc taatggtttt ttcaatcatc
    18181 gccaccaggt ggttggtgat tttggcgggg gcagagagga cggtggccac ctgcccctgc
    18241 ctggcattgc tttccagaat atcggcaaca cgcagaaaac gttctgcatt tgccactgat
    18301 gtaccgccga acttcaacac tcgcatggtt gttacctcgt tacctttggt cgaaaaaaaa
    18361 gcccgcactg tcaggtgcgg gcttttttct gtgtttcctg tacgcgtcag cccgcaccgt
    18421 tacctgtggt aatggtgatg gtggtggtaa tggtggtgct aatgcgtttc atggatgttg
    18481 tgtactctgt aatttttatc tgtttgtgcg ctatgcctat attggttaaa gtatttagtg
    18541 acctaagtca ataaaatttt aatttactca cggcaggtaa ccagttcaga agctgctatc
    18601 agacactctt tttttaatcc acacagagac atattgcccg ttgcagtcag aatgaaaagc
    18661 tgaaaaatac ttactaaggc gttttttatt tggtgatatt tttttcaata tcatgcagca
    18721 aacggtgcaa cattgccgtg tctcgttgct ctaaaagccc caggcgttgc tgtaaccagt
    18781 cgaccagttt tatgtcatct gccactgcca gagtcgtcag caatgccatg actcgttcgc
    18841 gtaaagcttg cagttgatgt tggtctgccg ttgtgtcact tttcgccggt tgttgtatta
    18901 atgttgctaa ttgatagcaa tagaccatca ccgcctgccc cagattgagc gaaggataat
    18961 ccgccaccat cggcacacca gtaagcacgt cagccaacgc taactcttcg ttagtcagcc
    19021 cggaatcttc gcgaccaaac accagcgcgg catggctcat ccatgaagat ttttcctcta
    19081 acagcggcac cagttctact ggcgtggcgt agtaatgata tttcgcccga ctacgcgcag
    19141 tggtggcgac agtgaaatcg acatcgtgta acgattcagc cagtgtcggg aaaactttaa
    19201 tattatcaat aatatcacca gatccatgtg cgacccagcg ggtggctggc tccaggtgtg
    19261 cctgactatc gacaatccgc agttcgctaa aacccatcgt tttcattgcc cgcgccgctg
    19321 ccccaatatt ttctgctctg gcgggtgcga ccaggataat cgttatacgc atattgccac
    19381 tcttcttgac caaataaccg cgaacagggt gatcactgtc aacttattac gcggtgcgaa
    19441 tttacaaatt cttaacgtaa gccgcagaaa aagcccttta cttagcttaa aaaaggctaa
    19501 actattttct gactgcacta acggtttaat tgttaaaaaa tgctacatat ccttctgttt
    19561 acttaggata attttataaa aaataaatct cgacaattgg attcaccacg tttattagtt
    19621 gtatgatgca actagttgga ttattaaaat aatgtgacga aagctagcat ttagatacga
    19681 tgatttcatc aaactgttaa cgtgctacaa ttgaacttga tatatgtcaa cgaagcgtag
    19741 ttttattggg tgtccggccc ctcttagcct gttatgttgc tgttaaaatg gttaggatga
    19801 cagccgtttt tgacactgtc gggtcctgag ggaaagtacc cacgaccaag ctaatgatgt
    19861 tgttgacgtt gatggaaagt gcatcaagaa cgcaattacg tactttagtc atgttacgcc
    19921 gatcatgtta atttgcagca tgcatcaggc aggtcaggga cttttgtact tcctgtttcg
    19981 atttagttgg caatttaggt agcaaacatg cagaccccgc acattcttat cgttgaagac
    20041 gagttggtaa cacgcaacac gttgaaaagt attttcgaag cggaaggcta tgatgttttc
    20101 gaagcgacag atggcgcgga aatgcatcag atcctctctg aatatgacat caacctggtg
    20161 atcatggata tcaatctgcc gggtaagaac ggtcttctgt tagcgcgtga actgcgcgag
    20221 caggcgaatg ttgcgttgat gttcctgact ggccgtgaca acgaagtcga taaaattctc
    20281 ggcctcgaaa tcggtgcaga tgactacatc accaaaccgt tcaacccgcg tgaactgacg
    20341 attcgtgcac gcaacctgct gtcccgtacc atgaatctgg gtactgtcag cgaagaacgt
    20401 cgtagcgttg aaagctacaa gttcaatggt tgggaactgg acatcaacag ccgttcgttg
    20461 atcggccctg atggcgagca gtacaagctg ccgcgcagcg agttccgcgc catgcttcac
    20521 ttctgtgaaa acccaggcaa aattcagtct cgtgctgaac tgctgaagaa aatgaccggc
    20581 cgtgagctga aaccgcacga ccgtactgta gacgtgacga tccgccgtat tcgtaaacat
    20641 ttcgaatcta cgccggatac gccggaaatc atcgccacca ttcacggtga aggttatcgc
    20701 ttctgcggtg atctggaaga ttaatcggct ttaccaccgt caaaaaaacg gcgcttttta
    20761 gcgccgtttt tatttttcaa ccttatttcc agatacgtaa ttcatcgtcc gttgtaactt
    20821 ctttactggc tttcattttc ggcagtgaaa acgcatacca gtctatatta cgagtcacaa
    20881 acatcatgcc ggccagcgcc accaccagca cactggttcc caacaacagc gcgctatcgg
    20941 cagagttgag cagtccccac atcactccat ccagcaacaa cagcgcgagg gtaaacaaca
    21001 tgctattgcg ccagcctttc aataccgcct gcaaataaat accgttcatt aacgccccaa
    21061 tcagactggc gattatccat gccacggtaa aaccgatatg ttcagaaagc gccagcaaga
    21121 gcaaataaaa catcaccaat gaaagcccca ccagcaaata ttgcattggg tgtaaacgtt
    21181 gcgcggtgag cgtttcaaaa acaaagaacg ccataaaagt cagtgcaatc agcagaatgg
    21241 cgtacttagt cgcccggtcc gttaattggt attgatcggc tggcgtcgtc actgcgacgc
    21301 taaacgcggg gaagttttcc cagccggtat cattgcctga agcaaaacgc tcaccgagat
    21361 tattagcaaa ccagctgctt tgccattgcg cctgaaaacc tgactcgcta gcttcccgtt
    21421 tggctggtag aaaatcacct aaaaaactgg gatgcggcca gttgctggtt aaggtcattt
    21481 cgctattacg cccggcaggc accacagaaa gatcgccggt accgcttaaa ttcagtgcca
    21541 tattcagctg caggttctgc ttccgccagt ccccttcggg taaagggata tgcacgccct
    21601 gcccgccttg ctctaacccg gtaccgggtt caatggtcag tgccgttccg ttaacttcag
    21661 gcgctttcac cacaccaata ccacgcgcat ccccgacgct aatcacaata aagggcttgc
    21721 ctaagatgat atttggcgcg ttgagctcgc taagacgcga aacatcgaaa tcggctttta
    21781 gcgttaaatc actgtgccag acctgaccgg tataaatccc tatcttgcgt tcttccacgt
    21841 tctgattgcc atcaaccatc aatgactcag gtaaccaaaa atggataaaa cttcgtttcc
    21901 gctccacggt tttatcctct tcctgcaccg tataaagctc ggtcacagga atagcgataa
    21961 gcggcccaac gagtttttgc ggcccgctgg tactttggcg aatcgcatct tccacatcgc
    22021 tacggtaatc ggcacgttcg acaatcactt gccgaatcag cattatcgga atcaacaaca
    22081 gcaatactgc accaaacaag gtggtcattt tccagaacag gggggatttc aacatagtct
    22141 tctcctttgc aatggcagca ggatacgaag gctatgtggg aagaatttga agctatgtga
    22201 agtgacggtg aagtcgtagc gaggccagca cgccaccttc ctgcacgttg cgcagcgtga
    22261 cttcgccgtt aaacaaacgg gcgacctcac tgacgaacgc caaccccaga ccgctgcttt
    22321 tttgcccatt tgcacgcggc aaagagtaaa agcgttcaaa aatacgtgac agcgcgtagt
    22381 caggaatacc actaccggta tccagcacct taagcgtgac gtgttcctga tccacttcgg
    22441 cgcttagcgt tatgcaaccg ctctcgggag taaaatcgat ggcgttatcc agtaaattcc
    22501 ccagcgcctg atccagtaac gccggttcag cagcaacatt aacctcggtg ggcataacat
    22561 gcaaagtgat gtttttttct gccaactgca cggtgcgcgc ttcgctgacg cggcgaaata
    22621 atgccgccac atcaacagca gtcagaacga cttcctgacg attctccagt cttgcctggc
    22681 gtagtaacgt ttccaccagc gcctgcattc gcgcattttg tgtcagaatg ttgtcagtaa
    22741 aacgagccac tacttcaggc ggcggacctt cgcgtaaaat ttccgccgcg ccacgaatcg
    22801 ccgccagtgg gctttttagc tcatgggtta acgcataaac atactgctca atatagtttt
    22861 tcccttccag cttcacgcgc atactttcca gcgcctgcgc gagtttacgc agctcgctac
    22921 taccgagatc gggaagagga acgggcttat tgtcagtgac ggaatcagca tagcgagtga
    22981 gccgggcaat ggagcggttg atccaccaaa ccatgcctgc gccaatcacc agtgcaatcc
    23041 ccaacaaaat ggcgctggcc cataaaattc gccgctcgct gcgtttaatg accggagcca
    23101 tcgccgcgtt cggtttgcct acgctcaaaa cgccaataag ccgcgagccg cccataatcg
    23161 gcgcggcaac atacatcacc gaactttcgg gatcggcagg attttgcaac gtgctgcgcg
    23221 caccatactg accacgcaac gttagccaga cgtcattcca gcgcgaataa tcctgtccaa
    23281 cggctttatt tgccgaatcg aacaatactt tgccatgcgc atcggtcata tagacatgat
    23341 attcattgcg cactttgtta atgccaccaa tattggcgcg aaacgggcga tgttgtagct
    23401 gattaaacgc ctgcgccagt tgcccatgcg ttgggtcccc agagagcaaa tcgggacgcg
    23461 ccagctccgc aagtaacgtt gccgtgtcga tcaacgtccc ctccgttgct cttcgcacgc
    23521 ccggtttaac ttctttgaca aaaatagcca gtacgaacca ggccgccacc gccaccagta
    23581 aaaaatagcc cagcagcaac cgcatgccga tacgcattac aagcccctca ggctatatcc
    23641 catgccgcga tgagtattaa tcggtgaaag atcggggttg atggcgcgca gcttggcacg
    23701 cagcgtttta atgtggatat cgacggtgcg atcgtaggtg tcctgcgcat cttcccacac
    23761 gctatccatt agttgctggc gggaccagac gcggcctggc gacttgagta acgtcttcag
    23821 caataaaaac tcataccgcg tcagcgttaa tggcgtgtca aaccagctga tctgcgccgc
    23881 gggttcattc aattcaaaat gaccaatacg gatgacggga gacggcgtcg agaacttctt
    23941 cacccgacgc agtaaggtgc gcaccctggc gcacacttcg cggggtgaaa acggtttagc
    24001 cacatagtcg tcagcgccaa tttccagccc aagcaggcga tcgacctctt cacttcgggc
    24061 cgtcaggaac agtacaggta acgccggata gagcgccagt aactggcggc acaattcaaa
    24121 gccactgata tcaggcaaac caacgtcgag aatcatgacg tcgggaacct gctggcgagc
    24181 tttatccagc accggcaaac ctcgctcaaa gacctcgacg gcaaaacctt cctgctgcaa
    24241 catgtagacc agcgtgtcgg ctatcccttg ctcatcttcc actaaccaga ccgtttcccg
    24301 ttgcataaat cgcctctgtt attgccgcca cggcatgaca ggaaccgcgc taatcgcgtt
    24361 tttcggcgaa ccttctacaa ctttgtcgga gtaagccaga taagcgagcg cgttgcgttt
    24421 ggcgtcataa aagcgcacga cctgtaacga cttaaagacc agggacgtgc gttttttgaa
    24481 tactacctcg ccctgagctt tgccgttttt aatacgatcc gacagttcaa tcggcccgac
    24541 ttgctgacaa gaaatggccg catcggaggt atcttctgcc aggcccaatc cccctttaat
    24601 accaccggtt ttcgcccggc tcacataaca ggtgacattt ttcacatcgg gatcgtcaaa
    24661 ggcttccaca acaattttgt gatccgggcc gatcatttta aatacggtgt cgaccgaacc
    24721 aatctcttct gcatgagcca atggccccag cattatcagg cttaaagaca ggatcaaatg
    24781 cttgtatttc atattgttac catttttatc tattacacga gcaattagtt cgtcacggta
    24841 aataacatat tgattaagaa caaaatctca attcttttat ttccgtcaag ccctaaaaca
    24901 tactctacta agaaaaaaac actgaatgct aaaacagcaa aaaatgctat tatccaatta
    24961 cctgatgtca ggtgctcgtt gttgaaagga tgaggatatt ttatggatca ggccggcatt
    25021 attcgcgacc ttttaatctg gctggaaggt catctggatc agcccctgtc gctcgacaat
    25081 gtagcggcga aagcaggtta ttccaagtgg cacttacaga gaatgtttaa agatgtcact
    25141 ggccatgcta ttggcgcgta tattcgtgct cgtcgtttgt cgaaatcggc ggtcgcacta
    25201 cgcctgactg cgcgtccgat tctggacatc gcgctgcaat accgcttcga ctctcaacag
    25261 acatttaccc gcgcattcaa gaagcagttt gcccagactc ctgcacttta ccgccgttct
    25321 cctgaatgga gcgcctttgg tattcgcccg ccgctacgtc tgggtgaatt cacaatgcca
    25381 gagcacaaat ttgtcaccct ggaagatacg ccgctgattg gtgttaccca gagctactcc
    25441 tgttcgctgg agcaaatctc tgatttccgc catgaaatgc gttatcagtt ctggcacgat
    25501 tttctcggca acgcgccgac cattccgccg gtgctctacg gcctgaatga aacgcgtccg
    25561 agtcaggata aagacgacga acaagaggta ttctatacca ccgcgttagc ccaggatcag
    25621 gcagatggct atgtactgac ggggcatccg gtgatgctgc agggcggcga atatgtgatg
    25681 tttacctatg aaggtctggg aaccggcgtg caggagttta tcctgacggt atacggaacg
    25741 tgcatgccaa tgctcaatct gacgcgccgt aaaggtcagg atattgagcg atactacccg
    25801 gcagaagacg ccaaagcggg agatcgccca attaatctgc gctgtgaact gctgattcct
    25861 atccgccgtt aacgctgcag ctcatctaat gcaggggcgt ccagatgcga gatgtcccct
    25921 gcggtttcaa cgacccagcc agacgccagc cacaggcttt cctgataatc cacgcgcgaa
    25981 atagaacagt tacgcagacg taagcggcgc tctgcccatg ctggtaatcc gagaatcgta
    26041 ctcaccagac atcccagtgc aataccatgg ctcaccagca gcggtcggct tccctgcggt
    26101 aagtcccggc aagattccag tgccgcattg acgcgatcgc tgagctcctg cattgactcg
    26161 ccttcaggaa tacgcccgtc aacggtgcca ttgaccagct gccgacgcca gttctcttct
    26221 tcttcggtca gagaatcgat atgtcttttt tccagcacac ccatgtttaa ttcacgcagg
    26281 cgagaatcaa agatgatgtc acagccgcag gcctgggcga tgatttccgc cgtacgccgg
    26341 gtgcgtccta aatcgctact gataatatgc gtaatgccaa gctctttggc acgggttgcc
    26401 acctgcatcg cctgttgctc acctttggcg gtcagcgggc tgtcagactg gccctgaata
    26461 cgtcgctcgg cgttccactg cgtttcaccg tggcggacta ggtatacctg taacatgctt
    26521 tttttccgta tactgctgta acgttaattt tctgagtaat gctgattatg caccaagttg
    26581 tctgtgcgac caccaatccc gctaaaattc aggccattct gcaggcattt cacgagatct
    26641 tcggcgaagg atcctgccat attgcatccg ttgccgtcga gagcggtgta ccggaacaac
    26701 cctttggcag tgaggaaacg cgcgctggcg cacgaaatcg ggtagcaaat gcccgccgtt
    26761 tacttccaga ggctgatttt tgggtggcga ttgaagctgg cattgatggc gacagcactt
    26821 tcagttgggt ggtgattgaa aacgccagcc agcgcggcga ggcgcgttcg gcaactttgc
    26881 cgttacctgc ggtgattctg gaaaaagttc gtgaaggcga ggcgcttggt cccgtgagtc
    26941 gcgttatacc ggtattgatg agattggtcg taaggaaggg gcgatcggcg tgtttaccgc
    27001 cgggaaactc actcgcgcca gcgtctatca ccaggcggtg atcctggcgc tgagtccgtt
    27061 tcacaatgcc gtgtatcagg cattgtagga cggataaggc gttcacgccg catccgacat
    27121 tcggtgcacg atgcctgatg cgacgctgcc gcgtcttatc aggcctacaa aatcaatcgc
    27181 ttttcagcaa cacctcttcc agccactgac gcagctccac gggcgcggct ttcaggctgt
    27241 tagatccacg cgtaatcgtc gcgatgcccg cgccgagttc attttttaac tcacgctgac
    27301 tcatttcgcc acgcaacagc tcttcgacaa tacgcacgcg agtccccaac gcttcgcgct
    27361 catctggcgt cagcatcagg tttaacaacg gtaaatggag atcgttttgg taggcattct
    27421 taagcaggtc gacaaaacgt aaccactcct ggtgacgctg ttctgccatc gctgctgaat
    27481 agggtgattg ttgggccata atatgtcgcc attgttagcg ggggaagcaa aatgactccc
    27541 ccggttgtac tcgctaaaga gtacgatagc atatcataaa cgtgcggatc agtaacgacg
    27601 tccccattcc gtggcgctca tcaacgtcgg tttatccccc atgaaatagc ggtagtaagc
    27661 atcataagcc agcacgttct tcacataacc gcgcgtctcg gagaacggaa tactctcgac
    27721 aaatgccaca gcgtcgatac gcccggcgct gttgccaagc caggttcgca cccgtccagg
    27781 tccggcgtta taagctgctg aggagaaaat acggttattg ccaaactgct gataaacata
    27841 ttgcaggtaa ctggtgccaa tgttgatgtt cgtttccgga tccagcaatt gcccagggct
    27901 gctataacca ggaatagaga acatcttcac cgtatgggtc gctgtaccag gcataatctg
    27961 catcaggccg ctggccccta ccggtgattt cactttcgga ttccaggcgc tctcctgacg
    28021 ggcaatcgcc atcgcatagc tttgcgggat ctctttccca ctggtgtaac gtttgaaaag
    28081 atcgttgtaa gccagcggga atcgctcttc cagatgatcc caaagcttcc cggcgatcgt
    28141 tgcctgaacg ctaagatccc accattggtt attgaaagca taccgagcca gttgagcctg
    28201 ctctgttttt gacttgctct tcaccagatt ggcccactcg ctacgcgcgg tattatcgag
    28261 attccagtac atcaactcgc gcacgcgcgc catctccggc ccctgagtca gggtgctgtc
    28321 aacattctgc ggcgctttat caatcttcag ctcatactct tcaccgatgc gttgtgctgc
    28381 aaccatcggg tagaaaccac gctgttgtat gagttgatgc aaaatctctt tcgcttcagc
    28441 ttcacgtccg cgttccagca ataaatccgc ctgccagtaa cgccattcat ctttctcttt
    28501 tgcttccatc ggcagacgcg ccagccaggt attcaggccg cgacgatcgc cggtgccaag
    28561 cgccattcgc acacggcgtt caatcaacga cgtggattgc gagcgcataa tggcgtcatc
    28621 gcgccatttc gcctgctcat cggtgacatc attgcccatc aaacgccagg cgacgatatc
    28681 gcgcagctcc tgaatctgat cttcattaag ctgctgcgcc tgggcaagcg acgggatcat
    28741 cagccgtgca ttttcggcat cctgccgcgc cacactggca aacgccaccg ccgccatttg
    28801 acgggtaaaa tcggtcgcgc cggttgtacg cgcgaaggtc agtaccgtat tagggttgtt
    28861 cgccagtgaa atgattgccg aggcgatagt ctggtaatcg gcaggcatct gccctgccag
    28921 cactgttacc aggcctgtgt tacccgcttt catcgccaga cggatacgct ctaaatacgc
    28981 cagcggatct tgtttacctg acgcacgcca gacgctaaat aacttgtcac aggcgttagg
    29041 ctggctcttg ccggttagcc acagctcttt cgccccttgc caggcttctt cactctgccc
    29101 ggtgttccat ttcgcatagt agtaattaca ttgcgcttcg gtagttccgg gcttttccgg
    29161 gctaaaggct aacaagccac gccagtcttc acgccgcgcc agttcattga cgaaacgaga
    29221 ttgcagcgtg cgagcgggag gaagcgtggg gttagcgcga acaaagttag tgaccgtcac
    29281 cgccggttga ttcatcaggt catcggtgat ctggcggtat tccaggtagg gataaagcgg
    29341 ataatccttc agtccaggca tcatttgttc gaccacatcc atctgtcgat tatcccaggc
    29401 ctgcttgatt tgcgcgtaac ggctacgctg ctcatccagt gagtcggctc gcgccacgct
    29461 gctgaccgtc agcagacaga caccggcagc caacagccgc caggtaactt gtttggcttt
    29521 ttccacaagc gcatcctcta agtgtaaata catcaatgca gcgtcatgcc gcgtaataga
    29581 tttatgctaa ccagtcattg ccgtttacgc cacgttacgg acactttttt acttttactg
    29641 cgaaggtgat cgaccaactg ggattaggcg gggaaacagg ctacacttcg cctttgaaaa
    29701 ctcatcatca cgataaacat agaggcgaag tccaacgtgg ctcaattcgt ttataccatg
    29761 catcgtgtcg gcaaagttgt tccgccgaaa cgtcatattt tgaaaaacat ctctctgagt
    29821 ttcttccctg gggcaaaaat tggtgtcctg ggtctgaacg gcgcgggtaa gtccaccctg
    29881 ctgcgcatta tggcgggcat tgataaagac atcgaaggtg aagcgcgtcc gcagccagac
    29941 atcaagattg gttatctgcc gcaggaaccg cagctgaacc cggaacacac cgtgcgtgag
    30001 tccattgaag aagcggtttc agaagtggtt aacgccctga aacgcctgga tgaagtgtat
    30061 gcgctgtacg ccgatccgga tgccgatttt gacaagctgg ccgctgaaca aggccgtctg
    30121 gaagagatca ttcaggctca cgacggtcat aacctgaacg tacagctgga gcgtgcggcg
    30181 gatgcgctgc gtctgccaga ttgggacgcg aaaatcgcta acctctccgg tggtgagcgt
    30241 cgtcgcgtgg cgttgtgccg cctgctgctg gaaaaaccag acatgctgct gctcgacgaa
    30301 ccgaccaacc acctggatgc cgaatccgtg gcgtggctgg aacgcttcct gcacgacttc
    30361 gaaggcaccg tggtggcgat tactcacgac cgttacttcc tcgataacgt tgcaggctgg
    30421 atcctcgaac tggaccgcgg tgaaggaatt ccgtgggaag gtaactactc ctcctggctg
    30481 gagcagaaag atcagcgtct ggcgcaggaa gcttcacaag aagcggcgcg tcgtaagtcg
    30541 atcgagaaag agctggagtg ggtacgtcag ggaactaaag gccgccagtc gaaaggtaaa
    30601 gcacgtctgg cacgctttga agagctgaac agcaccgaat accagaaacg taacgaaacc
    30661 aacgaactgt ttattccacc tggaccgcgt ctgggcgata aagtgctgga agtcagcaac
    30721 ctgcgtaaat cctacggcga tcgcctgctg attgatgacc tgagcttctc gatcccgaaa
    30781 ggggcgatcg tcgggatcat cggtccgaac ggcgcgggta aatcgaccct gttccgtatg
    30841 atctccggtc aggaaccgcc ggacagcggc accatcactt tgggtgaaac ggtgaaactg
    30901 gcatcggttg atcagttccg tgactcaatg gataacagca aaaccgtttg ggaagaagtt
    30961 tccggcgggc tggatattat gaagatcggc aacaccgaga tgccaagccg cgcctacgtt
    31021 ggccgcttta actttaaagg ggttgatcag ggtaaacgtg ttggcgaact ttccggtggt
    31081 gagcgcggtc gtctgcatct ggcgaagctg ctgcaggttg gcggcaacat gctgctgctc
    31141 gacgaaccaa ccaacgacct ggatatcgaa accctgcgcg cgctggaaaa cgccctgctg
    31201 gagttcccgg gctgtgcgat ggttatctcg cacgaccgtt ggttcctcga ccgtatcgcc
    31261 acgcacatcc tggactacca ggatgaaggt aaagttgagt tcttcgaagg taactttact
    31321 gagtacgaag agtacaaaaa acgcacgctg ggcgcagacg cgctggagcc gaagcgtatc
    31381 aagtacaagc gtattgcgaa gtaatgcgta aaatgccgga tgcggcgcga acgccttatc
    31441 cggcctacaa aaccagcaat ttcaatacgt tgtaaaactg taggcctgat aagacgcggt
    31501 aagcgtcgca tcaggcattg agcaccgaat gccggatgcg gcgtgaacac cttttccggt
    31561 ctacaacagc cttagcatcc tcaatttcgt ggcgggtcac ccgtgcaggt tttttcaaac
    31621 cttgtttaat ctcgacagcc tcttccagag aggttttcaa ctcatcaaaa aaactcattt
    31681 tatacctcgc ctttgagcag tttcgtcacg gttatccttg ctcccccatc atctcccgca
    31741 ccagctccac gcagcgcagg aaacggctgt cgtaatcgtc ctcttcaacc cgcacgaatt
    31801 cgatattgtt ctcttccagc atctccacca gcaagttctg gaactctttg cgatccaccg
    31861 aactgccgag gctgcgtaaa ccatccgcca cccacggcgt gttgttctcc agcaggatca
    31921 ccagatcgaa acggtattca tcaatcagcg cctgcacgaa cggatgctca cgcccttcgt
    31981 actttttgca gaacgcctgg gtagtgacaa aatcggtgtc aataaacgcc actttattgg
    32041 cgtatttcac tgcaaaatca atgtattgtg cgtgacccaa cgcgatttta tcgtagtcgg
    32101 aatactgcaa tgcgatttca tcaccgccga ggtgtgaaaa gacataatcg cggccatatt
    32161 cccacgcact ggtggtgttg aagatattgg caagtttgtt taccagggtg gatttaccgc
    32221 tcgactcgcc gccaaggatc gccacggtac gcacaaaaaa cggcttcact tcggtaggaa
    32281 tatattccca gtagcggaac gggttttcgc ggatctgcgc accgctgata ctcataaagg
    32341 tacgtttcgg atcgaccagc accgtctcga tccccagatg ttccatatac tgtggcgcat
    32401 cggcttcttc cgaggtgtag atcaggtccg gctggatccc cttttcagcc ataaactttt
    32461 tgatgccgtt gctccacaca tcccagccat gcggatacgg ctccatgccc tcttcgttga
    32521 aagcatgaat acggatattt ttctgatatt taaaggtttg caatagccag cgcagacgat
    32581 ccggcaccgt gggctgctgc gacatggcgc tgtcttcaaa caacgcgcgg tcgcgggtat
    32641 cgtcaaaacc cataatgata tgcagctcgt caacctggct acaggcgcgc tggataaggt
    32701 agatatgtcc ggtatgcagc gggtagaact taccgaatac gacaccaatg gttttcttct
    32761 gccggggaaa ttcaagcccc aaaaaacggt gcaatgcctc cagcttttgc gcgctggggc
    32821 ttttaatttt ggcattcagt aactggctta aatacccttt ggtcataccg ctggcatcag
    32881 ctacctgctg tagcgtgcag ccctgttgct tgatggcagt tttcaggtaa tcaaatgacg
    32941 acatatctcc ctccgtatct ctcattataa gtcgtcgaac acgctaagcg cgtcggagag
    33001 ttttttaacg ccaaaaatct gcatcccttc cggcgctttt ttcggtacgt tagccgccgg
    33061 aacaatcgcc cggcgaaaac cgtgtttcgc cgcttcagaa atccgctcct ggccactggg
    33121 caccgggcgg atctcccctg ccagcccgac ttcaccaaac accaccagat cctgtggcag
    33181 cggtctgtca cgcaggctgg aaaccatcgc cagcagtaac gctaagtcgg cgctggtttc
    33241 ggttaccttc acgccgccga ccacgttcac aaacacatcc tgatcggcca tttgcagacc
    33301 accgtgacgg tgcaagacag ccagcaggat tgccagacgg ttttgttcca gccccactgc
    33361 cacgcggcgc ggattcgcca ttatcgagtg atcgaccagc gcctgaatct ccaccagcaa
    33421 cggacgcgtt ccttcccaca ccaccatcac ggagctgccg gaggtcactt catctccgcg
    33481 acttaagaaa attgccgaag ggttgctgac ttcacgcagc ccctgttcgg tcatcgcgaa
    33541 gacgcccagc tcattcaccg cgccgaagcg gtttttatgg ctgcgtaagg tgcgaaaacg
    33601 ggagtcggca tcgccatcca gaagcaccga acagtcgata cagtgttcca gcactttcgg
    33661 gccagccagc gaaccatctt tggttacgtg tccaaccatg acaatcgcca caccgcgcgt
    33721 tttggcgaaa cgcgtcagat aagccgccgt ttcacgcacc tgcgccacgc taccaggcga
    33781 tgattgtaca tccgccatat gcatcacctg gatcgagtca attaccatca gcttcggttg
    33841 ctcttcttcg gcaatcaggc agatctgctc gatgctggtt tccgacaaca tattgagatt
    33901 gtcagtcggc aggccaaggc gatgagcgcg cattgccacc tgttgcagcg actcttcgcc
    33961 ggtgacatac agcgttttca tctgctgggc cagtttgcac agcgtttgca gcaacagcgt
    34021 ggatttcccc gcaccagggt taccgccaat cagaatggca cttcctggca ccacgccgcc
    34081 tcctagtacg cggtcgaact ctttaaatcc ggtggaaaaa cgcggcagct cttcaaggct
    34141 gatatcggag agtttctgga cttttgccac cccggcgcta ccggcatagc cgctgagacg
    34201 ctcgttacgc gccaccgttg gcgacgcagc aagacgcacc tcggtgatgg tgttccaggc
    34261 atgacaggca ctgcactgcc cctgccagcg cggataatcg gccccgcatt cattacaaac
    34321 aaaggcgcgt tttggagctt ttgccacggt gtacctcgtt aatgctgtgc cgcccgcagg
    34381 atgacgggca ggcaattact tctgattcag gctgccagag aggatgcaga atacccccat
    34441 caggtcagcg tgacggatgg tgacttccgc cttttcattc acttttggct tggcatggta
    34501 ggcaatcccc agccctgctg ctttgatcat cggcaggtca ttagctccat cgccaatcgc
    34561 cacggtctgc gccagcggga tttcatactc ctgcgcgagg cgagtcagag ttttcgcttt
    34621 gtactgcgcg tctacgatgt cgccgatcac attgccggta aatttaccgt ccatgatctc
    34681 cagttcattg gctaccacgg ctgtcaggcg cagcttgtcg cgcaggtatt cagcaaagaa
    34741 agtaaagccg ccggaggcaa tcgccacttt ccagcccagc gtttccagct tgagcaccag
    34801 ctgcgttaag cctggcatca gcggcagatt ttcacgcacc tgttgcagaa tattggcgtc
    34861 agcgcctttc agcgtcgcca cgcggctgcg caggctggcg gtaaaatcga gttcgccgcg
    34921 catcgcccgt tcggttacct ccgccaccat ctcgcccgtt ccggccagct tggcaatttc
    34981 atcaatacat tcaatctgga tggcggtgga atccatgtcc atcaccagca aacccggcgt
    35041 gcgcaggtgc gggattttcc ccagcggggc gacatccagc tgcgcttcgt gggccaggcg
    35101 tgtagcccgt gcggtgagtg aacctgccag acgaatcacc tgataatctt ccacgcacca
    35161 ggcggcaaca atcaccatcg ccgcacccag tttgctctgg tattgggtca gacgctgttt
    35221 atccagccca cgaccataca gcagccagcc gctacgacct gcgtggtaat ccagtggcat
    35281 cacttcatca ccacttaatg aaagaggcag acccggccat aaagagacat cttcaggcag
    35341 gtcgcaccag gtaatgttag gcattaaggc tcctgtaaaa tcgttcgaag cagggaaaat
    35401 aacgcatgag gctaccttgt atccattgct tctggcaaca ttaagtctca aattttcaaa
    35461 gggtggaaga tggctcgcac aaaactgaaa ttccggctac atcgggcagt gattgtcctg
    35521 ttctgtcttg ccttgttagt ggcactgatg cagggggcgt catggtttag tcaaaaccac
    35581 cagcgacagc gtaatccaca gctggaagaa ctggcccgca ccctggcgcg tcaggtgacg
    35641 ctgaacgttg caccgctgat gcgtaccgac tcaccggatg aaaaacgcat tcaggcgatc
    35701 ctcgatcagt taacagatga aagccgtatc ctcgacgcgg gtgtgtatga cgaacaaggc
    35761 gatcttatcg cacgttctgg cgaaagcgtc gaagtgcgcg atcggctggc gctcgacggt
    35821 aaaaaagcag gcggctattt taaccagcag attgtcgagc caattgcggg taaaaacgga
    35881 ccgctcggct atctgcgcct gacactcgac acccatacgc tcgccaccga agcccaacag
    35941 gtggataaca ccactaacat tttacgcctg atgttgctgc tctcactggc aatcggcgta
    36001 gtgctgaccc gcacgctgct acagggtaaa cgcacccgct ggcagcaatc gcccttcctg
    36061 ttaaccgcca gcaaaccggt gccggaagag gaagaaagcg agaaaaaaga gtgacccatt
    36121 actacaagaa aggaaatcgt tatgtccaca ttacgcctgc tcatctctga ctcttacgac
    36181 ccgtggttta acctggcggt ggaagagtgt atttttcgcc aaatgcccgc cacgcagcgc
    36241 gttctgtttc tctggcgcaa tgccgacacg gtagtaattg gtcgcgcgca gaacccgtgg
    36301 aaagagtgta atacccggcg gatggaagaa gataacgtcc gcctggcgcg gcgcagtggc
    36361 ggtggcggcg cggtgttcca cgatctcggc aatacctgct ttacctttat ggctggcaag
    36421 ccggagtacg ataaaactat ctccacgtcg attgtgctca atgcgctgaa cgcgctcggc
    36481 gtcagcgccg aagcgtccgg acgtaacgat ctggtggtga aaaccgtcga aggcgaccgc
    36541 aaagtctcag gctcggccta tcgcgaaacc aaagatcgcg gcttccacca cggcaccttg
    36601 ctactcaatg ccgacctcag ccgcctggca aactatctca atccggataa aaagaaactg
    36661 gcggcgaaag gcattacttc agtgcgttcc cgcgtgacca acctcaccga gctgctgccg
    36721 gggctcacca tgagcaggtt tgcgaggcca taaccgaggc ctttttcgcc cattatggcg
    36781 agcacgtgga agcggaaatc atctccccgg acaaaacgcc agacttgcca aacttcgccg
    36841 aaacctttgc ccgtcagagt agctgagagt ggaacttcgg tcaggctccg gcattctcgc
    36901 atctgctgga tgaacgcttt agctggggcg gcgtggaact gcatttcgac gttgaaaaag
    36961 gccatatcac ccgcgcccag gtatttaccg acagcctcaa ccccgcgccg ctggaagccc
    37021 tcgccgggcg actgcaaggc tgcctgtacc gcgcggatat gctgcaacaa gagtgcgaag
    37081 cgctgttggt tgacttcccg gaccaggaaa aagagctacg ggagttgtcg acgtggatag
    37141 cgggggcggt aaggtaactc gctgattaaa atatccaagg aaggaaatat gatcgatctg
    37201 gaaaaccagg aacgggagat tatcaattta atgttcagcc agcgtatttc ctggctagcc
    37261 gccgtgcgta tcaggcataa attatcgctt gctgaagtaa gtaaaatgtt aggtatctcg
    37321 attaattctc taaaacagat cgaaaagacg gaacggctta gctcaaacat caaaagcaaa
    37381 atggcagaaa tttatggttg tccaccagag ttgctgatat gcccttcctg gatgacggct
    37441 gaacataaat gaatagattc gtgaatagat acttgaatgt tttttatttt ctatccatat
    37501 gaaaaataat agtaaatcta taaatcagtt gctattgatg aatagatact aaaaagccgg
    37561 agcagtcccc cactccggct taacacgaca atcgcctttc agcgaaacac aattactctt
    37621 tatcgcccag cagaacggat tccagtgcga ttttgatcat gtcgttgaag gtggtctgac
    37681 gctcagcggc agtggtctgc tcgtgagtgc ggatgtggtc agacacggtg cagatggtca
    37741 gggctttcgc gccaaactct gcagccacgc cgtagatacc agccgcttcc atttccacgc
    37801 cgaggatgcc gtatttttcc atcacgtcga acatttcgcc gtccggagag tagaacaggt
    37861 cagcggagaa caggttaccc acgcgagcat caacgcccag tgctttagct gcatctactg
    37921 cgttacgcac catgtcaaag tcagcgatag cggcaaagtc atggtcttta aaacggatgc
    37981 ggttaacttt ggaatcggtg caggccccca taccgataac gacgtcgcgc agttttacgt
    38041 gcggcagaac tgcgccacag gaaccaacgc ggataatttt cttcacgccg aaatcggtga
    38101 tcagttcttt ggtgtagatg gagcaggacg ggatacccat accgtgaccc attacggaaa
    38161 ttttgcggcc tttgtaagta ccagtgaagc ccagcatacc gcgaacgttg ttcacttcac
    38221 gggcatcttc aaggaaagtt tcagcaatat acttcgcacg cagcgggtcg cctggcatca
    38281 aaactacgtc agcgaaatcg cccatttctg cattaatgtg tggggtagcc attgttttat
    38341 cctttttgtg acataacaaa ggcggagtca acgctccgcc caaatccatc agaacatggc
    38401 tttgccatat tccatatcag aagtaccaaa atattttgcc agagtctggc cgatatccgc
    38461 gaaggtttca cggtgaccca gtgagcccgg ttttactttc gggccatata ccagtaccgg
    38521 aatgtgttca cgcgtgtggt cagtaccggt ccaggtcgga tcgcaaccgt ggtcagcggt
    38581 gaggatcagg atgtcgtcat cgcgcagcag agacatcagc tccggcagac ggcggtcgaa
    38641 cagttccaga cccgcagcat aaccggcgac gtcgcggcgg tggccccagg aagagtcgaa
    38701 gtcaacgaag ttggtgaaga cgatggtgtt atcacccgct tctttcatct ctttgatggt
    38761 ggcgtcaaac agcgcgtcca agccagtcgc tttcactttt ttggtgatac cgcagttggc
    38821 gtagatgtct gcaattttac cgacagaaac cacctggccg tgtttttcat caaccagttt
    38881 ctgcagcacg gtcggtgctg gcggctcaac agccaagtcg tgacggttac cggtacgctg
    38941 gaagttacca gctttgtcac cgataaacgg acgagcgata acacgaccga tattgtagcc
    39001 gccgttggtc agctcttcac gggcgatttc gcacagttcg tagagtttat ccagaccgaa
    39061 agtttcttca tggcaggcaa tctggaacac ggagtcagcg gaggtataga aaatcggctt
    39121 gccggttttc atgtgctctt cgcccagttg atccagaatg accgtaccgg aagagtggca
    39181 gttaccgagg taacccggca gattagcgcg ttcgaccagt ttatccagca gctcttgcgg
    39241 gaagctgttt tcgtgatcgg agaaatatcc ccactcaaac agaaccggga caccggcgat
    39301 ttcccagtga ccagacgggg tatctttacc ggatgacatt tcgtgcgccc atgcgtacgc
    39361 gccgataact tcagcgttgc cgtccattcc cgccggaatg aaaccggtag aaccttcgtg
    39421 tgctttcgcc agccccagac gggtcagatt tggcagattg agcggccctt tacgaccatt
    39481 atcagcttcg cctttggcac aagcttctgc gatatgaccc agggtgtcag ccccgacgtc
    39541 accaaagcgt tctgcatctt ctgtagcgcc gatgccgaat gagtccagca ccataataaa
    39601 tgcacgtttc atatgttctc cgtactcagt gcttcaaatt taaatgatca gatcagtata
    39661 ccgttattcg ctgatacggc gatagacagt tggtgtgctt tccggtgcgt tatcggcaag
    39721 tttaattgcc gctttcaccg ctttcgccgc ttcctgccag ctgttttcgt ctttcgcgag
    39781 gataaccgcc agcggacgct gaccgtctac ctggtcgccc agacgcgcca tatcagtaaa
    39841 gccgacgctg taatcgatgg tgtcagatgc ctgacgacga ccgccgccca ttgcaactac
    39901 tgccatcccc agcgcgcggg tatccatttc actgacaaaa ccttcggtat cagcatagac
    39961 tgctttcgtc agcatcgctg tcggcagata cttcgcgtag ttctcaacga agtcggtcgg
    40021 gcctttttgt gccgctacca tacgaccaaa gacttctgcc gctttaccgt tgtccagcac
    40081 cgcctgcaat ttcgcgcgcg cttcggcgtc atctttcgcc agtttgccgg agatcaacat
    40141 ctccacgcac agcgccatcg tgacatcaaa cagacgcggg ttacgatact cacccgtcag
    40201 gaactgcacc gcttcacgaa cttcaaccgc gttacctgca ctggaggcca gtacctgatt
    40261 catgtcggtg agcagcgcgg tggtgcgcac gccagcgccg ttagccacgc caacaatcgc
    40321 ttcggcaagg gcttcagaga gttcgtaggt cggcataaac gcgccgctac ccactttcac
    40381 gtccatcacc agcgcgtcca gaccttccgc cagtttcttc gccaggatcg aggcagtgat
    40441 cagcgggatg gagtccacgg ttgcggtaat atcacgggtc gcgtagaaac gtttatcagc
    40501 cggagccagt gaactggtct gaccgataat cgccacgccg acgtctttaa taatttcgcg
    40561 gaaacggttg tcatccggga aaatgtcgaa gccagggatg gattccagtt tgtcgagcgt
    40621 accgccagta tgaccgaggc cgcgaccgga gatcatcgga atatagccgc cgcaggctgc
    40681 gaccatcggc cccaacatca gcgaagtgac atcgccgacg ccgccggtcg agtgtttatc
    40741 aacaatcggg ccattcagat gcaggctttt ccagtcgaga acggttcctg aatctcgcat
    40801 cgccatggtc agcgagacac gctcaggcat tgtcatatcg tggaagaaaa tggtcatcgc
    40861 gagggcggca atctgccctt cggaaatagt gttgtcgcga ataccgttga taaagaaacg
    40921 aatttcttca tcgctcagcg catgaccatc acgtttttta cgaataattt cttgtgcgag
    40981 aaacaaggta acctcccgag gaaaagagta tgaggcggct tttgccggat gcgtctacgc
    41041 cttatccggc ctacgggtag atacgtctcg taggcctgat aagcgcagcg catcaggcgt
    41101 aaagcatctt acttagtagc tgctggcgct cttaccgtcg ccgtggccca gcgctttcag
    41161 caggcttgcc agcaggctgg aagcaccaaa gcggtagtga cgcgcatcag cccagtcagc
    41221 accgaacagt tcatctgcga tggcgagata tttctgcgca tcttccgcag tacgcacgcc
    41281 gcccgccggt ttgaaaccaa cggttttttc tacgcccata tcacggatca cttccatcat
    41341 gatgcgagcg ctttccggcg tcgcgttcac agccacttta ccggtagagg ttttgatgaa
    41401 gtccgcaccc gctttgatgg agatttcaga cgctttacgg atcagcgctt cgtctttcag
    41461 ttcgccggtt tcgatgatca ctttcagcag tacattcgct gccgcgcaag cctctttaca
    41521 ggctttcacc aggtcaaaac caacctgctc gtcacccgcc atcagcgcgc ggtacgggaa
    41581 caccacgtca acttcatcag caccgtaggc gattgccgca cgggtttctg ccagcgcgat
    41641 ttcgatgtcg tcgttaccgt gtgggaagtt ggttaccgta gcgatacgga tttccggggt
    41701 gccctgctct ttcagagttt tgcgagcaat cgggataaaa cgaggataga tacagatagc
    41761 ggcggtattg ccgaccggag ttttggcctg atgacacaga gcaattactt tctcgtcggt
    41821 gtcgtcgtca ttcagggtgg tcaggtccat caatttcagt gcacgcaggc tgcttgcttt
    41881 cagatcagtc atttcattct cctggcttgt cgccaataaa attcaccttg cgagtttgtt
    41941 agtattctaa catctactcc gcaacacact tcgatacaca tcacaattaa ggaaatctac
    42001 ttacaagttt gcatcactgt aatgcgatct ggttcaaata attcactttc aaatgaatgc
    42061 gtcagtggtg gcaaacgcat caggatcaaa gtgaacatca cgaaacttct tacaatggcg
    42121 catcaccttc ggcataaaag gaaaagaaga tgcccacttc tcatgaaaat gcactgcaac
    42181 aacgttgcca gcaaattgtc accagcccag tgcttagccc ggagcagaag cgccattttc
    42241 tggcactgga agcagaaaac aatctgcctt acccacagct tcctgccgaa gcccgccgcg
    42301 cgctggatga aggtgtaatc tgcgatatgt ttgaaggtca tgcgccgtac aaaccgcgct
    42361 atgtcttacc cgattacgct cgttttctgg cgaacggttc cgaatggctg gagctggaag
    42421 gcgcgaaaga tcttgatgac gcactctctc tgctgaccat tctttaccac cacgtaccgt
    42481 cggtcacatc gatgccggtc tacctggggc aactggatgc gttgttgcaa ccgtatgtta
    42541 gaattctaac acaaaacgag atcgatgttc gaataaaacg tttctggcgt tacctcgaca
    42601 gaaccctgcc agatgccttt atgcacgcca atatcggccc gtctgattcg cccattaccc
    42661 gcgcgatctt acgtgcagac gcgcaggttt caccgaacct gacctttatc tacgatcctg
    42721 aaatcacccc tgatgacctg ctgctggaag tggcgaagaa catctgtgaa tgtagcaaac
    42781 cgcacatcgc caacggtccg gtgcatgata aaattttcac aaaagggggc tacgggattg
    42841 tgagctgtta caactcactg ccgctggcgg gtggtggcag cacgctggta cgccttaacc
    42901 tgaaagccat tgccgagcgc agcgaatcgc tggatgactt ctttacgcgc actctaccgc
    42961 actactgcca gcagcagatc gccatcatcg atgcgcggtg tgaattcctc tatcaacaat
    43021 cacacttctt tgagaatagc ttcctggtga aagaagggct gattaaccct gaacgttttg
    43081 tgccaatgtt tggcatgtat gggctggcgg aagcggttaa cttgctgtgt gagaaagaag
    43141 ggattgccgc gcgctacggt aaagaagccg ccgcaaatga agtgggttat cgcatcagcg
    43201 cgcaactggc ggagtttgtc gccaataccc ccgtgaaata tggctggcaa aaacgcgcca
    43261 tgttacacgc acagtcgggg atcagttccg atatcggcac cacgccgggc gcgcgtttgc
    43321 cgtatggcga tgagccagat ccgatcaccc atctgcaaac tgtcgccccg catcatgctt
    43381 attattattc cggcatcagc gacattctga cgctcgacga aaccatcaaa cgtaacccgc
    43441 aggcactggt acagctttgc ctcggtgcct ttaaagccgg aatgcgtgaa tttaccgcca
    43501 atgtcagcgg taacgatctg gttcgcgtta ccggttatat ggtgcgtttg tcggatttag
    43561 aaaaatatcg cgccgaaggt tcacgcacca acaccacctg gctgggcgaa gaagccgcac
    43621 gcaacactcg tattctggaa cgccagccgc gcgtgataag ccatgaacag cagatgcgct
    43681 ttagtcagta agattatccc cttctcctgc gttgacgggc caggcagtcg tctggctctg
    43741 tttttgcagg gctgcaatct gcgctgcaaa aactgtcaca atccgtggac gatgggacgt
    43801 tgcaatgact gtggggagtg cgtgccacag tgtccgcatc aggcgttgca gattgttgac
    43861 ggcaaagtgg tgtggaacgc tgtggtctgc gagcaatgtg atacctgcct gaagatgtgt
    43921 ccgcaacatg ccacaccaat ggcgcaatcc atgagcgtgg acgaagtgct tagccatgtg
    43981 cgcaaagcgg tgctgtttat tgaagggata acggtaagcg gcggcgaagc cacgacccaa
    44041 ctgccgtttg tagtggcgct gtttactgct atcaaaaacg atccgcaact gcgccatctc
    44101 acctgtctgg tggacagtaa cggtatgttg agcgaaaccg gctgggaaaa attactcccg
    44161 gtgtgtgacg gcgcaatgct cgatctcaaa gcatggggta gcgaatgtca tcaacaatta
    44221 accggacgcg ataatcaaca gattaagcgc agcatctatt tgctggcaga gcgcggcaag
    44281 ctggcggaac tgcgtttgct gatgatccca ggccaggtgg attatttgca acacatcgaa
    44341 gaactggcgg cgtttatcaa gggacttggt gatgttccgg ttcgcctgaa cgcgtttcat
    44401 gcccacggcg tgtatggcga ggcgcaaagc tgggcgagcg ccacgccgga agacgttgag
    44461 ccgttggctg atgcattaaa ggtgcgcggg gtgagccggt tgatatttcc ggcgctctat
    44521 ttgtgacatg ccggatacgg cctacggttc ggcacagact tgtaggcctg ataagacgcg
    44581 tcaagcgtcg catcaggcat tgtgcgccaa ctgccggatg cggcgtgaac gccttatccg
    44641 gcctacggtt cggcacaaac ctgtaggcat gataagacgc gttaagcgtc gcatcaggca
    44701 ttatgcgccg actgccggat gctgcctacg aagggcaaac tacccacgaa cgttaaacac
    44761 cgcatacgtg ttattaagca acacttcggc aatctcatcc gcgggttccg ggcgtaactc
    44821 gcaaagcacg gcaaacacac gggcagcctg ttccgggcgg ttaggctgcc cctgaaaacc
    44881 gttgagcggc atatccggcg catcggtttc cagcaacaac gatgccagcg gtaattttgc
    44941 gatgacatcg cgggttttac tggcgcgtgg ataggtgata gtaccgccta cgccgatttt
    45001 gtagcccagc tgtacaaacc gttcagcctg ttgctggctg ccagaaaatc cgtggacaac
    45061 accagcacgc ggcaaatcgt ggcgtttaag atgcatcgcc agtttgtcgt gcgtgcgccg
    45121 tgaatgcagg atcaccggca gatcgtagcg tttcgccagt ttcagttgtt cgtcgagtaa
    45181 ccactgctgc ctctcaaatt gcggatcgtc gccgaagaga tccagaccga tctcccccac
    45241 cgccaccacc ttcgccggac gcctttccag cgcctgctgt agctgctcaa gagacacatc
    45301 gctatgtttt tccaacatac caggatgcaa gcccaatacg gcatacagcg gttgataatt
    45361 ttccgctaac gcctgcacac gggcaaaatt ttccgcctct gtcgccggaa caatgatctt
    45421 gcctacgccc gcttgtgccg cgcgttgcag gctggcctct tcatcgccaa taaaaggcgg
    45481 gaaatcaaaa tggcagtgag tgtcgataaa acggcaaatc aagccagatc ctcattgtta
    45541 aatgtcgggt cgttcgcctg cggagcgtcg ctcacttcgg caaccagcgt atcgttggcg
    45601 actggcgcag gcggaatgac aatcgattcc ggcgtcacca ctggcaccag atggcgggta
    45661 agcggcgctt tttcagttaa tagcttgcca accgtggcga ggaaataacg accgcataaa
    45721 cgcccaagtt tatagtcttc acgcagcgcc ggaatccgac tgccaagcgc gatactatgt
    45781 aatggcttcg gcggataaat ttcggatatc cgcagcttgc ccggtggttt ctcaataaat
    45841 tgctgaatgt cacgatagct ggtttcatga tgctgcacca gattgaccag cggctgcagg
    45901 ctactgtcac ccagccagcg ttccatgcgt ttgaaccact gcggcgtgta gtacatttgt
    45961 gacggcacag tgcggatgac gaccaacgtt ttagcgccct gccttgccgc ctctttaacc
    46021 ggaatcgcat cactgatccc gccatccagg tagttaatgc cttccagcga cactccgcta
    46081 cgataaaagc caggtatcgc actggaggcg cgaatcacat ccagccagtt ttgtttggtt
    46141 ggtaaaaagt aattcggcgc gtagtcatcc tgacgacagg cgcacatata aaacgatttg
    46201 ccgctgtcaa acaaccgcgc ggcggtatcc atttgcaacg gcatctggct tgcggtggcc
    46261 tccaccagcc agtcgagatc gataagattt cctccacgga caaagcgcaa tggatcgaaa
    46321 aattcgcgtt ttgtggtata gcgcatgatg actttgcgcg cgtaaccggg ctgattgcag
    46381 ataaacgccg agaggttctg cgccccggca gatgtgccga gataaagatc gaaaggatta
    46441 aactgcgcgc gcataaactc atccagcacg ccagccgtga aaattccacg ctgtccgccg
    46501 ccttcgcaca ccagagctat tcgtccaggc tggaacggcc ttagcgacaa cggcgcaata
    46561 ttaccaagcg ttacaggtat tcgctgcccc acttctgcct tcctgttttt gttgtgaaag
    46621 aaagacaaag taacgcaatt tattattcgc taaaaccgca aaagccagtc cgctggactg
    46681 gctttggata aaattttctt aatggtctga aatctagggt cgttttcggc ccatgaacaa
    46741 actcactagg aacagaataa tcccgacgac aaagacaatt ttagctgcgc ctgcagcggt
    46801 accggccaga ccaccaaacc caagtgcggc ggcgattaac gcgataacca gaaatatgat
    46861 gccccaacga aacataagcg tctcctttac catagttaat gtcaccgctt gatatgagcg
    46921 tgaaaatcac tcaatgcgat atttttagtg tggtgcacat cgcgcctccc gacaaagttc
    46981 gggaggacga attacttagt tttcagatca tttttaacgc ttttcacacc atctaccgct
    47041 ttggcgatac tttcagcacg gtcactttgt gcctgagaat ctacggtacc ggagagctga
    47101 accacgccat cggtggtttc aactttcact ttacgtgaag ggacgatatc gtccgccagc
    47161 agtttggctt tgatttcact ggtggtggcg gtgtcacccg cgtagccctt caccgagcct
    47221 tctttagcgt cgcgaacgtg cagtttgtcg ctgacagagg tcacgccttc aacgcctttc
    47281 gccactttca ctgcctcttc ggcctgggcc tgactttcaa cgaaaccgct cagggtcacg
    47341 actttttgat cggttttgac agagatatcg gtgctcttga tgttgtcatg atccaccagc
    47401 gccgccttca ctttcgcggt gatggcgctg tcatccatga aattaccgac tttattcata
    47461 gagctatcga ctttttgccc tgcgctttca ttggtagtct gcgcgttgtt ttccgcgtag
    47521 gcagagccgg tcgcgacggc agaggtcaac attacagcca gcagagtttt cgaaatcttc
    47581 agtcttgtca tagtcatcga tttattcctg tatgtttgct cgtaatttga gcctagcaac
    47641 acgaggttgc attgctgaat agggagagac ttcaccctct acagaagtca atggtcgcca
    47701 tcacaaaagc gatgggtgat gaataacgac cattacagcc tctgaatcag ttattaatat
    47761 cggtagaatg acaatcgacg gctttagata ctaatatcta cgcattgaac ggtatttaac
    47821 gccatcagaa atgtcatcac tttgttaaat atagatcaca attttgaaac cgctcgggat
    47881 atcagcgaaa acataagcaa aagtgaatgt tttaagaaca ttccgtaagc ggctaataag
    47941 gaagggaaat tgacagggcg cagcggctac cgcgccctgg aggcaagaat taatgctcgc
    48001 gggtctggtg gaactgaacg tccggataac gttcctgtgc caggcgcagg ttgaccatgc
    48061 tggtagcgat gtaagcgagg ttatcgccgc catcaagcgc cagttggctt tcgttcttac
    48121 gcttgaactc ttcgaatttc ttcgcgtctg cacattctac ccagcgggca gtggcaacgt
    48181 tgactgactc atacactgct tcaacgttgt attcgctctt caggcgcgct accaccacat
    48241 caaactgcag cacaccaacc gcaccaacga tcagatcgtt gttggagatt ggacggaaca
    48301 cctgcaccgc gccctcttcg gaaagctgta ccagcccttt gagcagctgt ttttgcttca
    48361 gcggatcttt caggcggata cgacggaaca gttctggtgc gaagttcgga ataccggtga
    48421 acttcatcat ctcaccctgg gtaaaggtgt cgccgatctg aatggtgccg tggttgtgca
    48481 ggccgaggat atcgcccgga tacgcttctt caacgtgcga acggtcaccc gccataaagg
    48541 tcagcgcgtc ggagatcacc acatctttcg cagtgcgcac ctggcgcagt ttcatgcctt
    48601 tttcatattt accggacacc acacgcataa acgccacgcg gtcgcggtgt ttcggatcca
    48661 tgttggcctg aattttaaat acgaagccgg taaatttatc ttcgctcgct tctacggtac
    48721 gggtatcggt ctgacgcggc atcggcgcag gcgcccactc caccaggcca ttcagcatat
    48781 gatcaacgcc gaagttaccc agcgcagtac cgaagaatac cggagtgatt tcgcccgcaa
    48841 ggaacagctc tttgtcgaac tcgttagacg cgcctttaac cagttccagt tcgtcacgca
    48901 gctgctgtgc cagatcttca ccaaccgcag catcgagatc cgggttattc agccctttaa
    48961 caatgcggac ttcctgaatg gtgtgccctt taccgctctg atagagatag gtttcatctt
    49021 tataaaggtg gtaaacgcct ttaaacagct tgccgcagcc aattggccaa gtgatcggtg
    49081 cgcagccaat tttcagctcg ttctcaactt catcgagcag ttccatcggg tcgcggatat
    49141 cacggtcaag tttgttcata aaggtgagga tcggcgtgtc gcgcagacgg gtaacttcca
    49201 tcagcttacg ggtacgatct tcaacacctt ttgcggcgtc gataaccatc aggcagcagt
    49261 ccaccgccgt cagggtacga taggtatctt ccgagaagtc ttcgtgcccc ggggtgtcga
    49321 gcaggttaac caggcaatcg tgatacggaa actgcatcac agacgtagta atggagatcc
    49381 cacgctgctt ttccatctcc atccagtccg acttagcgtg ctggttggaa ccacggcctt
    49441 ttactgtacc ggcggtctga atggcctgtc cgaacagcag caccttctcg gtgatggtag
    49501 ttttaccggc gtccgggtga gaaataatgg caaaagtgcg gcgcttcgcc acctctttca
    49561 aataaggaga caacgtcata attctttctt cttgagtaag cggcatcgcg ccgcgcttgt
    49621 tgaaaacgaa aaattgcggc tattttaccc atcaacgggg gggaggcaat cagtgtttac
    49681 acaggagctg ctccagttcg tgcaacgaag aaacggtcca ggtgggcgcg atgccttctg
    49741 gttgctcgcg attgtgcgca ttcagccagc aggtcgcaag cccggcgttg atgccaccga
    49801 gaatatcgga ctcggcagtg tcgccaacca tcagcacgcg tgaacggtca ggattgcccg
    49861 cctgttccag cgcataatcg aaaattttct tattcggttt ggcaacgcca acttcttcgg
    49921 aaatcaccag caaatcgaag taatcacgca ggcccgtccg ttccagacgc acttgctgca
    49981 aggcgctaaa accgttggtg atgatgccga ttttagcgtt gctacgaatg gcgttaagca
    50041 gagaaaccgc gcccggcaac ggcgtacaga tttccgccat cgcattaata aaggcatcgt
    50101 taagcaagcc tgcttcaact ttcagccgtt cggcccagct ctcgaaacgc ccgtgctgaa
    50161 gctgtaatga agtgatcgcg ccgttttgat aatccaccca cagtggcttg ttaacggcct
    50221 gatagtcctg aaaatcttca gcggtaaagg tgacgctgta atcaagaaac atccgctgca
    50281 ggccggtgaa tgagtcaaag gtaaacagcg tttcatcggc atcaaagaaa atccagtccc
    50341 acttcattat tccaccttgt cttacatact gattggcaac gccatgatga tggcgtcttc
    50401 gcgaccgtcc gtggtggggt agtaattgcg gcgaatcgtc gcctcgttaa agcctaaact
    50461 ttcgtacagg gcaatggcgg cagcatttga ggcacggact tccagccata gtgtcgccac
    50521 gccgcgtttt tccagttcgt cgatcagatg ttccagcagc gccctaccca gcccctgacg
    50581 ctgatagtcg ggatcgaccg caatattgaa caatgtagct tcatccagca ccacttgcgt
    50641 aatcgcaaac gccgccattt tgccgttttg cgttaactga aagttgagat aacgctcgcc
    50701 ctggttgctg gcaaaggttt tttcactcca cggaaaggcg tgggcgcgtt gttcaatgtg
    50761 gtaagccgct ggtaaatcag tcgtttcgag ggaagaaatc gtgttcatat gtgcaaattt
    50821 gttgccataa cgcggcgcgt gccgttgggt ttgcccgtaa ttcggtgagc gccggtgatg
    50881 ccacctgagc gccttccaat gatagcggtt cgtcagtacc caaccgccag ctgttgcagc
    50941 gactgccttg cggcagcatc gcgatttttt ctggcgtcag ttgcagcact tgatcggggc
    51001 tgacggttaa tgcgcgcaga acatcgctca ctaaaggatc agtcagggcg ggaagatcgt
    51061 ttgccaccat caccagacgg acgtgtgccg ggctcgcaat ggcaatctcc ccctgcaacg
    51121 cgccagggcg acgcagcgac cactgggtaa tgcccagttg ctgtaactgc cagtctcgtc
    51181 gggatgtcat agcgaatcgc tcctgttgtc aggggcgcaa atatagcaaa ttcgtcgata
    51241 ccgcgccaac atatggctat aatcgccgcc agtatcaatt gaggagcatt ccatgtctgc
    51301 atttaccccg gcaagtgaag tcttgctgcg tcacagtgat gatttcgaac aaagccgtat
    51361 tctgtttgcc ggagacttac aggatgacct gcccgcgcgt ttagataccg cggccagccg
    51421 tgctcatacc cagcaattcc accattggca ggtgttaagc tgccagatgg gggataacgc
    51481 ccgttttagt ctggtcgcca cggcgaatga tgtcgcagat tgcgatacgc tgatttacta
    51541 ctggccgaag aacaaaccgg aagcccagtt ccagttgatg aatttacttt ctctgctgcc
    51601 agtggggaca gatatttttg tcgttggcga gaaccgcagc ggcgtgcgca gcgctgagca
    51661 gatgctggca gattatgcgc cgttgaataa agtcgacagc gctcgtcgct gtggcctcta
    51721 ttttggtcgt ctggaaaaac agccagtatt tgatgcggat aaattctggg gcgaatacag
    51781 cgtcgatggc ctgacggtca aaacgctgcc tggcgtgttt agccgcgacg gtctggatgt
    51841 cggtagccag ttgctgctct cgacgttaac tccgcacacg aaaggtaaag tgctggatgt
    51901 cggctgtggc gcgggtgtac tttcagttgc ctttgcgcgc cattcgccga aaattcgtct
    51961 caccttgtgc gatgtctctg ccccggcggt agaagccagc cgcgcaacac ttgcagccaa
    52021 cggtgttgaa ggtgaagtct ttgccagcaa cgtcttttcc gaggtgaaag gttgttttga
    52081 tatgatcatc tccaacccgc cgttccacga tgggatgcaa accagcctgg atgcggcgca
    52141 aacgctgatt cgtggcgcgg tgcgtcatct taatagcggc ggcgagctgc gaattgtagc
    52201 gaacgccttc ctgccttacc cggacgtgct ggatgagaca tttggcttcc atgaagttat
    52261 cgcgcaaacc gggcgcttca aggtatatcg cgccattatg acccgccagg cgaagaaagg
    52321 ttgagtatct gatatctgtc gccgcatacc gtgtcggatg cggcgtgaac gccttatccg
    52381 acctacaaaa cctgcacgtt agcccttcgt aggccagata agacgcgcca gcgtcgcatc
    52441 tggcatctgc ccccgcatgc ctaccaaacc tgcacgctag tttcctgatg gacatttttc
    52501 cagcaattac acatcaggcg ataattaact attgacgaaa agctgaaaac cactagaatg
    52561 cgcctccgtg gtagcaattc tttttaagaa ttgatgg
//
LOCUS       DAJTRC010000018        49524 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.018, whole genome shotgun sequence.
ACCESSION   DAJTRC010000018 DAJTRC010000000
VERSION     DAJTRC010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.018"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..1224
                     /locus_tag="OO357_001147"
     CDS             <1..1224
                     /locus_tag="OO357_001147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5886166.1"
                     /translation="FELPEITDNELLVSVISDSVCLSTWKAALLGSEHKRVPDDLENH
                     PVITGHECAGVIVEVGKNLTGKYKKGQRFVLQPAMGLPSGYSAGYSYEYFGGNATYMI
                     IPEIAINLGCVLPYHGSYFAAASLAEPMCCIIGAYHANYHTTQYVYEHRMGVKPGGNI
                     ALLACAGPMGIGAIDYAINGGIQPSRVVVVDIDDKRLAQVQKLLPVDLAASKGIELVY
                     VNTKGMSDPIQMLRALTGDVGFDDIFVYAAVPAVVEMADELLAEDGCLNFFAGLTDKN
                     FKVPFNFYNVHYNSTHVVGTSGGSTDDMKEAIALSATGQLQPSFMVTHIGGLDAVPET
                     VLNLPDIPGGKKLIYNGVTMPLTAIADFAEKGKTDPLFKELARLVEETHGIWNEQAEK
                     YLLAQFGVDIGEAAQ"
     gene            1221..2186
                     /gene="yggF"
                     /locus_tag="OO357_001148"
     CDS             1221..2186
                     /gene="yggF"
                     /locus_tag="OO357_001148"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HCR5886167.1"
                     /translation="MMSLAWPLFRVTEQAALAAWPQTGCGDKNKIDGLAVTAMRQALN
                     DVAFRGRVVIGEGEIDHAPMLWIGEEVGKGDGPEVDIAVDPIEGTRMVAMGQSNALAV
                     MAFAPRDSLLHAPDMYMKKLVVNRLAAGAIDLSLPLADNLRNVARALGKPLDKLRMVT
                     LDKPRLSAAIEEATQLGVKVFALPDGDVAASVLTCWQDNPYDVMYTIGGAPEGVISAC
                     AVKALGGDMQAELIDFCQAKGDYTENRQIAEQERKRCKAMGVDVNRVYSLDELVRGND
                     ILFSATGVTGGELVNGIQQTANGVRTQTLLIGGADQTCNIIDSLH"
     gene            2208..2717
                     /gene="fumE"
                     /locus_tag="OO357_001149"
     CDS             2208..2717
                     /gene="fumE"
                     /locus_tag="OO357_001149"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarase E"
                     /protein_id="HCR5886168.1"
                     /translation="MATLTEDDVLEQLDAQDNLFSFMKTAHSILLQGIRQFLPSLFVD
                     NDEEIVEYAVKPLLAQSGPLDDIDVALRLIYALGKMDKWLYADITHFSQYWHYLNEQD
                     ETPGFADDITWDFISNVNSITRNATLYDALKAMKFADFAVWSEARFSGMVKTALTLAV
                     TTTLKELTP"
     gene            2714..3427
                     /locus_tag="OO357_001150"
     CDS             2714..3427
                     /locus_tag="OO357_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside/nucleotide kinase family protein"
                     /protein_id="HCR5886169.1"
                     /translation="MKIELTVNGLNVQAQYHDEEIERVHKLLLRMLAALQTVNPQRRT
                     VVFLCAPPGTGKSTLTTFWEYLAQQDPELPAIQALPMDGFHHYNSWLDAHQLRPFKGA
                     PETFDVAKLAENLRQVVEGDCTWPQYDRQKHDPVEDALHVTAPLVIVEGNWLLLDDEK
                     WCQLAQFCDFSIFINAPAAALRERLVGRKLAGGVSLADAEAFYDRTDGPNVRRGLEES
                     LPANLTLMMTATGEYRLVD"
     gene            complement(3399..4076)
                     /locus_tag="OO357_001151"
     CDS             complement(3399..4076)
                     /locus_tag="OO357_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate/molybdate ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5886170.1"
                     /translation="MLTLNKISYRWPGAATDCLCDISLQLKQGEWLALTGDNGAGKST
                     LLRVMAGLLTPTAGTVMLQQQAMKNLKNRQRAAKVGVLFQEAENQLFHSTVADEIAFG
                     LKLQKCPADEITQRTHAALQCCQLADTANSHPLDLHNAQRRMVAVACLEALSPPLLLL
                     DEPSRDFDENWLSVFESWLEKCRQRGTSVVAISHDAAFTRRHFSRVVRLEDGVIRNVN
                     PPDDIHP"
     gene            complement(4070..4747)
                     /locus_tag="OO357_001152"
     CDS             complement(4070..4747)
                     /locus_tag="OO357_001152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HCR5886171.1"
                     /translation="MVTLEQFRYCPTHSTHPPFCYDFHYVKPGMVAIFGDNGSGKSTL
                     AQLMAGWYPDFLPREITGTGTLLGTPIGHLPLNEQSATIQLVQQSPYLQLSGCTFSVE
                     EEVAFGPENLCLAEAEIMARIDAALTLTECQPLRHRHPATLSGGETQRVIIACAIAMQ
                     PKLLILDEAFSRLTPQASEMLLQRLQHWAFERGSLIILFERHRTPFLNYCQQAWQLQN
                     GALQPLC"
     gene            complement(4735..5118)
                     /locus_tag="OO357_001153"
     CDS             complement(4735..5118)
                     /locus_tag="OO357_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HCR5886172.1"
                     /translation="MQCFIRALFASRLPPGVAYLFAGPLLVVEQLKRQLAIIHEAQRA
                     RGVPLDEGWYQRLRAMPALIIPLTHNALNDLAVRGAALDMRAFRIHNRRTTLWAPADS
                     TLQRVARYTMILLMLTEFGAWIWLR"
     gene            complement(5134..5442)
                     /locus_tag="OO357_001154"
     CDS             complement(5134..5442)
                     /locus_tag="OO357_001154"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886173.1"
                     /translation="MHPFTSLTLWALAACTTLILPAQTILPIYSAATFFCLIALKATR
                     RRAKYVVWLMFSLGAGLWLVHGGWLPEWLSGTPRSPERWSHAITLWLLILAIVSTSQL
                     "
     gene            complement(5443..6021)
                     /locus_tag="OO357_001155"
     CDS             complement(5443..6021)
                     /locus_tag="OO357_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HCR5886174.1"
                     /translation="MARSHFSSQALVLIVISIAINMIGGQLASMVKLPIFLDSIGTLI
                     SAVLLGPVIGMLTGLLTNLLWGLLTDPIAAAFAPVAMVIGLVSGWLARAGWFRTLPKV
                     VVSGVIITLAVTVVAVPLRTALFGGVTGSGADLFVAWMHSMGQNLVESVAITVIGANL
                     VDKILTAVIVWLLLRQLPIRTTRHFPAMAAVR"
     gene            complement(6044..6475)
                     /locus_tag="OO357_001156"
                     /pseudo
     CDS             complement(6044..6475)
                     /locus_tag="OO357_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
     gene            6847..7866
                     /gene="epd"
                     /locus_tag="OO357_001157"
     CDS             6847..7866
                     /gene="epd"
                     /locus_tag="OO357_001157"
                     /EC_number="1.2.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="erythrose-4-phosphate dehydrogenase"
                     /protein_id="HCR5886175.1"
                     /translation="MTVRVAINGFGRIGRNVVRALYESGRRAEITVVAINELADAAGM
                     AHLLKYDTSHGRFAWEVRQERDQLFVGDDAIRVLHERSLQSLPWRELGVDVVLDCTGV
                     YGSREHGEAHIAAGAKKVLFSHPGSNDLDATVVYGVNQDQLRAEHRIVSNASCTTNCI
                     IPVIKLLDDAYGIESGTVTTIHSAMHDQQVIDAYHPDLRRTRAASQSIIPVDTKLAAG
                     ITRFFPQFNDRFEAIAVRVPTINVTAIDLSVTVKKPVKANEVNLLLQKAAQGAFHGIV
                     DYTELPLVSVDFNHDPHSAIVDGTQTRVSGAHLIKTLVWCDNEWGFANRMLDTTLAMA
                     TVAFR"
     gene            7916..9079
                     /gene="pgk"
                     /locus_tag="OO357_001158"
     CDS             7916..9079
                     /gene="pgk"
                     /locus_tag="OO357_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005761248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HCR5886176.1"
                     /translation="MSVIKMTDLDLAGKRVFIRADLNVPVKDGKVTSDARIRASLPTI
                     ELALKQGAKVMVTSHLGRPTEGEYNEEFSLLPVVNYLKDKLSNPVRLVKDYLDGVDVA
                     EGELVVLENVRFNKGEKKDDETLSKKYAALCDVFVMDAFGTAHRAQASTHGIGKFADV
                     ACAGPLLAAELDALGKALKEPARPMVAIVGGSKVSTKLTVLDSLSKIADQLIVGGGIA
                     NTFIAAQGHDVGKSLYEADLVDEAKRLLTTCNIPVPSDVRVATEFSETAPATLKSVND
                     VKADEQILDIGDASAQELAEILKNAKTILWNGPVGVFEFPNFRKGTEIVANAIADSEA
                     FSIAGGGDTLAAIDLFGIADKISYISTGGGAFLEFVEGKVLPAVAMLEERAKK"
     gene            9294..10373
                     /gene="fbaA"
                     /locus_tag="OO357_001159"
     CDS             9294..10373
                     /gene="fbaA"
                     /locus_tag="OO357_001159"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016505616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HCR5886177.1"
                     /translation="MSKIFDFVKPGVITGDDVQKVFQVAKENNFALPAVNCVGTDSIN
                     AVLETAAKVKAPVIVQFSNGGASFIAGKGVKSDVPQGAAILGAISGAHHVHQMAEHYG
                     VPVILHTDHCAKKLLPWIDGLLDAGEKHFAATGKPLFSSHMIDLSEESLQENIEICSK
                     YLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALYTQPEDVDYAYTELSKISPRFT
                     IAASFGNVHGVYKPGNVVLTPTILRDSQEYVSKKHNLPHNSLNFVFHGGSGSTAQEIK
                     DSVSYGVVKMNIDTDTQWATWEGVLNYYKANEAYLQGQLGNPKGEDQPNKKYYDPRVW
                     LRAGQTSMIARLEKAFQELNAIDVL"
     gene            10564..11424
                     /gene="mscS"
                     /locus_tag="OO357_001160"
     CDS             10564..11424
                     /gene="mscS"
                     /locus_tag="OO357_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small-conductance mechanosensitive channel MscS"
                     /protein_id="HCR5886178.1"
                     /translation="MEDLNVVDSINGAGSWLVANQALLLSYAVNIVAALAIIIVGLII
                     ARMISNAVNRLMISRKIDATVADFLSALVRYGIIAFTLIAALGRVGVQTASVIAVLGA
                     AGLAVGLALQGSLSNLAAGVLLVMFRPFRAGEYVDLGGVAGTVLSVQIFSTTMRTADG
                     KIIVIPNGKIIAGNIINFSREPVRRNEFIIGVAYDSDIDQVKQILTNIIQSEDRILKD
                     REMTVRLNELGASSINFVVRVWSNSGDLQNVYWDVLERIKREFDAAGISFPYPQMDVN
                     FKRVKEDKAA"
     gene            11563..12198
                     /gene="argO"
                     /locus_tag="OO357_001161"
     CDS             11563..12198
                     /gene="argO"
                     /locus_tag="OO357_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine exporter ArgO"
                     /protein_id="HCR5886179.1"
                     /translation="MFSYYFQGLALGAAMILPLGPQNAFVMNQGIRRQYHIMIALLCA
                     ISDLVLICAGIFGGSALLMQSPWLLALVTWGGVVFLLWYGFGAFKTAMSSNIELASAE
                     VLKQGRWKIIATMLAVTWLNPHVYLDTFVVLGSLGGQLDVEPKRWFALGTISASFLWF
                     FGLAILAAWLAPRLRTAKSQRIINLVVGCVMWFIALQLARDGIAHAQALFS"
     gene            12291..13031
                     /locus_tag="OO357_001162"
     CDS             12291..13031
                     /locus_tag="OO357_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative stress defense protein"
                     /protein_id="HCR5886180.1"
                     /translation="MKFKVIALAALMGISGMAAQANELPDGPHIVTSGTASVDAVPDI
                     ATLAIEVNVAAKDAATAKKQADERVAQYISFLELNQIAKKDISSANLRTQPDYDYQDG
                     KSILKGYRAVRTVEVTLRQLDKLNSLLDGALKAGLNEIRSVSLGVAQPDAYKDKARKA
                     AIDNAIHQAQELANGFHRKLGPVYSVRYHVSNYQPSPMVRMMKADAAPVSAQETYEQA
                     AIQFDDQVDVVFQLEPVDQQPAKTPAAQ"
     gene            13198..14148
                     /locus_tag="OO357_001163"
     CDS             13198..14148
                     /locus_tag="OO357_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5886181.1"
                     /translation="MDIFISKKMRNFILLAQTNNIARAAEKIHMTASPFGKSIAALEE
                     QIGYTLFTRKDNNISLNKAGQELYQKLFPVYQRLSAIDNEIHNSGRRSREIVIGIDNT
                     YPTIIFDQLISLGDKYEGVTAQPVEFSENGVIDNLFDRQLDFIISPQHVSARVQELEN
                     LTISELPPLRLGFLVSRRYEERQEQELLQELPWLQMRFQNRANFEAMIDANMRPCGIN
                     PTIIYRPYSFMAKISAVERGHFLTVIPHFAWRLVNPATLKYFDAPHRPMYMQEYLYSI
                     RNHRYTATIFSILLKIVTGQTINPASSRLQLNYGVSRRRG"
     gene            complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="OO357_001164"
                     /pseudo
     CDS             complement(14090..15568)
                     /gene="scpC"
                     /locus_tag="OO357_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311817.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="propionyl-CoA:succinate CoA transferase"
     gene            complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="OO357_001165"
     CDS             complement(15592..16377)
                     /gene="scpB"
                     /locus_tag="OO357_001165"
                     /EC_number="7.2.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311816.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA decarboxylase"
                     /protein_id="HCR5886182.1"
                     /translation="MSYQYVNVATINKVAVIEFNYGRKLNALSKVFIDDLMQALSDLN
                     RPEIRCIILRAPSGSKVFSAGHDIHELPSGGRDPLSYDDPLRQITRMIQKFPKPIISM
                     VEGSVWGGAFEMIMSSDLIIAASTSTFSMTPVNLGVPYNLVGIHNLTRDAGFHIVKEL
                     IFTASPITAQRALAVGILNHVVEVEELEDFTLQMAHHISEKAPLAIAVIKEELRVLGE
                     AHTMNSDEFERIQGMRRAVYDSEDYQEGMNAFLEKRKPNFVGH"
     gene            complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="OO357_001166"
     CDS             complement(16388..17383)
                     /gene="meaB"
                     /locus_tag="OO357_001166"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HCR5886183.1"
                     /translation="MINEATLAESIRRLRQGERATLAQAMTLVESRHPRHQALSTQLL
                     DAIMPFCGNALRLGVTGTPGAGKSTFLEAFGMLLIREGLKVAVIAVDPSSPVTGGSIL
                     GDKTRMNDLARAEAAFIRPVPSSGHLGGASQRARELMLLCEAAGYDVVIVETVGIAQS
                     ETEVARMVDCFISLQIAGGGDDLQGIKKGLMEVADLLVINKDDGDNHTNVAIARHMYE
                     SALHILRRKYDEWQPRGLTCSALEKRGIDEIWHAIIDFKTALTASGRLQQVRQQQSVE
                     WLRKQTEEEVLNHLFANEDFDRYYRQTLLAVKNNTLSPRTGLRQLSEFIQTQYFD"
     gene            complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="OO357_001167"
     CDS             complement(17376..19520)
                     /gene="scpA"
                     /locus_tag="OO357_001167"
                     /EC_number="5.4.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase"
                     /protein_id="HCR5886184.1"
                     /translation="MSNEQEWQQLANKELSRREKTVDSLVQQTAEGIAIKPLYTEADL
                     DNLEVTGTLPGLPPYVRGPRATMYTAQPWTIRQYAGFSTAKESNAFYRRNLAAGQKGL
                     SVAFDLATHRGYDSDNPRVAGDVGKAGVAIDTVEDMKVLFDQIPLDKMSVSMTMNGAV
                     LPVLAFYIVAAEEQGVTPDKLTGTIQNDILKEYLCRNTYIYPPKPSMRIIADIIAWCS
                     GNMPRFNTISISGYHMGEAGANCVQQVAFTLADGIEYIKAAISAGLKIDDFAPRLSLF
                     FGIGMDLFMNVAMLRAARYLWSEAVSGFGAQDPKSLALRTHCQTSGWSLTEQDPYNNV
                     IRTTIEALAATLGGTQSLHTNAFDEALGLPTDFSARIARNTQIIIQEESELCHTVDPL
                     AGSYYIESLTDQIVKQARAIIQQIDEAGGMAKAIEAGLPKRMIEEASAREQSLIDQGK
                     RVIVGVNKYKLDHEDETDVLEIDNVMVRNEQIASLERIRATRDDAAVTAALNALTHAA
                     QHNENLLAAAVNAARVRATLGEISDALEVAFDRYLVPSQCVTGVIAQSYHQSEKSASE
                     FDAIVAQTEQFLADNGRRPRILIAKMGQDGHDRGAKVIASAYSDLGFDVDLSPMFSTP
                     EEIARLAVENDVHVVGASSLAAGHKTLIPELVEALKKWGREDICVVAGGVIPPQDYAF
                     LQERGVAAIYGPGTPMLDSVRDVLNLISQHHD"
     gene            complement(19724..20617)
                     /gene="argP"
                     /locus_tag="OO357_001168"
     CDS             complement(19724..20617)
                     /gene="argP"
                     /locus_tag="OO357_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator ArgP"
                     /protein_id="HCR5886185.1"
                     /translation="MKRPDYRTLQALDAVIRERGFERAAQKLCITQSAVSQRIKQLEN
                     MFGQPLLVRTVPPRPTEQGQKLLALLRQVELLEEEWLGDEQTGSTPLLLSLAVNADSL
                     ATWLLPALAPVLADSPIRLNLQVEDETRTQERLRRGEVVGAVSIQHQALPSCLVDKLG
                     ALDYLFVSSKPFAEKYFPNGVTRSALLKAPVVAFDHLDDMHQAFLQQNFDLPPGSVPC
                     HIVNSSEAFVQLARQGTTCCMIPHLQIEKELASGELIDLTPGLFQRRMLYWHRFAPES
                     RMMRKVTDALLDYGHKVLRQD"
     gene            21000..21659
                     /gene="rpiA"
                     /locus_tag="OO357_001169"
     CDS             21000..21659
                     /gene="rpiA"
                     /locus_tag="OO357_001169"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HCR5886186.1"
                     /translation="MTQDELKKAVGWAALQYVQPGTIVGVGTGSTAAHFIDALGTMKG
                     QIEGAVSSSDASTEKLKSLGIHVFDLNEVDSLGIYVDGADEINGHMQMIKGGGAALTR
                     EKIIASVAEKFICIADASKQVDILGKFPLPVEVIPMARSAVARQLVKLGGRPEYRQGV
                     VTDNGNVILDVHGMEILDPIAMENAINAIPGVVTVGLFANRGADVALIGTPDGVKTIV
                     K"
     gene            21915..23147
                     /gene="serA"
                     /locus_tag="OO357_001170"
     CDS             21915..23147
                     /gene="serA"
                     /locus_tag="OO357_001170"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HCR5886187.1"
                     /translation="MAKVSLEKDKIKFLLVEGVHQKALESLRAAGYTNIEFHKGALDD
                     EQLKESIRDAHFIGLRSRTHLTEDVINAAEKLVAIGCFCIGTNQVDLDAAAKRGIPVF
                     NAPFSNTRSVAELVIGELLLLLRGVPEANAKAHRGVWNKLAAGSFEARGKKLGIIGYG
                     HIGTQLGILAESLGMYVYFYDIENKLPLGNATQVQHLSDLLNMSDVVSLHVPENPSTK
                     NMMGAKEISLMKPGSLLINASRGTVVDIPALCDALASKHLAGAAIDVFPTEPATNSDP
                     FTSPLCEFDNVLLTPHIGGSTQEAQENIGLEVAGKLIKYSDNGSTLSAVNFPEVSLPL
                     HGGRRLMHIHENRPGVLTALNKIFAEQGVNIAAQYLQTSAQMGYVVIDIEADEDVAEK
                     ALQAMKAIPGTIRARLLY"
     gene            23376..23435
                     /gene="ibsC"
                     /locus_tag="OO357_001171"
     CDS             23376..23435
                     /gene="ibsC"
                     /locus_tag="OO357_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin IbsC"
                     /protein_id="HCR5886188.1"
                     /translation="MMRLVIILIVLLLISFGAY"
     gene            23768..23827
                     /locus_tag="OO357_001172"
     CDS             23768..23827
                     /locus_tag="OO357_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5886189.1"
                     /translation="MMRLAIILIVLLLISFPAY"
     gene            complement(23928..24530)
                     /locus_tag="OO357_001173"
     CDS             complement(23928..24530)
                     /locus_tag="OO357_001173"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311809.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HCR5886190.1"
                     /translation="MTQLPELPLTLSRQEIRKMIRQRRRALTPEQQLEMGQQAATRMM
                     TYPPVVMAHTVAVFLSFDGELDTQPLIEQLWRAGKRVYLPVLHPFSAGNLLFLNYHPQ
                     SELVMNRLKIHEPKLDVRDVLPLSRLDVLITPLVAFDEYGQRLGMGGGFYDRTLQNWQ
                     HYKTQPVGYAHDCQLVEKLPVEEWDIPLPAVVTPSKVWEW"
     gene            complement(24551..24734)
                     /gene="ssrS"
                     /locus_tag="OO357_001174"
     ncRNA           complement(24551..24734)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="OO357_001174"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="OO357_001175"
     CDS             complement(24776..25105)
                     /gene="zapA"
                     /locus_tag="OO357_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HCR5886191.1"
                     /translation="MSAQPVDIQIFGRSLRVNCPPDQRDALNQAADDLNQRLQDLKER
                     TRVTNTEQLVFIAALNISYELAQEKAKTRDYAASMEQRIRMLQQTIEQALLEQGRITE
                     KTNQNFE"
     gene            25273..25851
                     /gene="ygfB"
                     /locus_tag="OO357_001176"
     CDS             25273..25851
                     /gene="ygfB"
                     /locus_tag="OO357_001176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YgfB"
                     /protein_id="HCR5886192.1"
                     /translation="MSIQNEMPGYNEMNQYLNQQGTGLTPAEMHGLISGMICGGNDDS
                     SWLPLLHDLTNEGMAFGHELAQALRKMHSATSDALQDDGFLFQLYLPDGDDVSVFDRA
                     DALAGWVNHFLLGLGVTQPKLDKVTGETGEAIDDLRNIAQLGYDEDEDQEELEMSLEE
                     IIEYVRVAALLCHDTFTHPQPTAPEVQKPTLH"
     gene            25877..27202
                     /gene="pepP"
                     /locus_tag="OO357_001177"
     CDS             25877..27202
                     /gene="pepP"
                     /locus_tag="OO357_001177"
                     /EC_number="3.4.11.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro aminopeptidase"
                     /protein_id="HCR5886193.1"
                     /translation="MSEISRQEFQRRRQALVEQMQPGSAALIFAAPEVTRSADSEYPY
                     RQNSDFWYFTGFNEPEAVLVLIKSDDTHNHSVLFNRVRDLTAEIWFGRRLGQDAAPEK
                     LGVDRALAFSEINQQLYQLLNGLDVVYHAQGEYAYADEIVNSALEKLRKGSRQNLTAP
                     ATMIDWRPVVHEMRLFKSPEEIAVLRRAGEITAMAHTRAMEKCRPGMFEYHLEGEIHH
                     EFNRHGARYPSYNTIVGSGENGCILHYTENESELRDGDLVLIDAGCEYKGYAGDITRT
                     FPVNGKFTQAQREIYDIVLESLETSLRLYRPGTSILEVTGEVVRIMVSGLVKLGILKG
                     NVDELIAQNAHRPFFMHGLSHWLGLDVHDVGVYGQDRSRILEPGMVLTVEPGLYIAPD
                     ADVPEQYRGIGIRIEDDIVITETGNENLTASVVKKPEEIEALMAAARKQ"
     gene            27199..28377
                     /gene="ubiH"
                     /locus_tag="OO357_001178"
                     /gene_synonym="visB"
     CDS             27199..28377
                     /gene="ubiH"
                     /locus_tag="OO357_001178"
                     /gene_synonym="visB"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-octaprenyl-6-methoxyphenyl hydroxylase"
                     /protein_id="HCR5886194.1"
                     /translation="MSVIIVGGGMAGATLALAISRLSHGALPVHLIEATAPESHAHPG
                     FDGRAIALAAGTCQQLARIGVWQSLADCATAITTVHVSDRGHAGFVTLAAEDYQLAAL
                     GQVVELHNVGQRLFALLRKAPGVMLHCPDRVANVARTQSHVEVTLESGEMLTGRVLIA
                     ADGTHSALATACGVDWQQEPYEQLAVIANVATSVAHEGRAFERFTQHGPLAMLPMSDG
                     RCSLVWCHPLERREEVLSWSDEKFCRELQSAFGWRLGQITHAGKRSAYPLALTRAARP
                     ITHRTVLVGNAAQTLHPIAGQGFNLGMRDVMSLAETLTQAHERGEDIGDYGILCRYQQ
                     RRQSDREATIGVTDSLVHLFANRWAPLVVGRNIGLMTMELFTPARDVLAQRTLGWVAR
                     "
     gene            28400..29602
                     /gene="ubiI"
                     /locus_tag="OO357_001179"
                     /gene_synonym="visC"
     CDS             28400..29602
                     /gene="ubiI"
                     /locus_tag="OO357_001179"
                     /gene_synonym="visC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent 2-octaprenylphenol hydroxylase"
                     /protein_id="HCR5886195.1"
                     /translation="MQSVDVAIVGGGMVGLAVACGLQGSGLRVAVLEQRVPEPLAVDA
                     LPQLRVSAINAASEKLLTRLGVWQDILARRASCYHGMEVWDKDSFGHISFDDQSMGYS
                     HLGHIVENSVIHYALWNKAQQSSDITLLAPTELQQVAWGENETFLTLKDGSMLTARLV
                     IGADGANSWLRNKADIPLTFWDYQHHALVATIRTEEPHDAVARQVFHGEGILAFLPLS
                     DPHLCSIVWSLSPEEAQRMQQASEDEFNRALNIAFDNRLGLCKVESARQVFPLTGRYA
                     RQFAAHRLALVGDAAHTIHPLAGQGVNLGFMDAAELIAELKRLHRQGKDIGQYIYLRR
                     YERSRKHSAALMLAGMQGFRDLFSGANPAKKLLRDIGLKLADTLPGVKPQLIRQAMGL
                     NDLPEWLR"
     gene            complement(29676..29759)
                     /locus_tag="OO357_001180"
     CDS             complement(29676..29759)
                     /locus_tag="OO357_001180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886196.1"
                     /translation="MSKNTKSKNHGIRKYNAKTEVKLVYFK"
     gene            30050..31144
                     /gene="gcvT"
                     /locus_tag="OO357_001181"
     CDS             30050..31144
                     /gene="gcvT"
                     /locus_tag="OO357_001181"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HCR5886197.1"
                     /translation="MAQQTPLYEQHTLCGARMVDFHGWMMPLHYGSQIDEHHAVRTDA
                     GMFDVSHMTIVDLRGSRTREFLRYLLANDVAKLTKSGKALYSGMLNASGGVIDDLIVY
                     YFTEDFFRLVVNSATREKDLSWITQHAEPFGIEITVRDDLSMIAVQGPNAQAKAATLF
                     NDAQRQAVEGMKPFFGVQVGDLFIATTGYTGEAGYEIALPNEKAADFWRALVEAGVKP
                     CGLGARDTLRLEAGMNLYGQEMDETISPLAANMGWTIAWEPTDRDFIGREALEVQREH
                     GTEKLVGLVMTEKGVLRNELPVRFTDAQGNQHEGIITSGTFSPTLGYSIALARVPEGI
                     GETAIVQIRNREMPVKVTKPVFVRNGKAVA"
     gene            31168..31557
                     /gene="gcvH"
                     /locus_tag="OO357_001182"
     CDS             31168..31557
                     /gene="gcvH"
                     /locus_tag="OO357_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HCR5886198.1"
                     /translation="MSNVPAELKYSKEHEWLRKEADGTYTVGITEHAQELLGDMVFVD
                     LPEVGATVSAGDDCAVAESVKAASDIYAPVSGEIVAVNDALSDSPELVNSEPYAGGWI
                     FKIKASDESELESLLDATAYEALLEDE"
     gene            31676..34549
                     /gene="gcvP"
                     /locus_tag="OO357_001183"
     CDS             31676..34549
                     /gene="gcvP"
                     /locus_tag="OO357_001183"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HCR5886199.1"
                     /translation="MTQTLSQLENSGAFIERHIGPDAAQQQEMLNAVGAQSLNALTGQ
                     IVPKDIQLATPPQVGAPATEYAALAELKAIASRNKRFTSYIGMGYTAVQLPPVILRNM
                     LENPGWYTAYTPYQPEVSQGRLEALLNFQQVTLDLTGLDMASASLLDEATAAAEAMAM
                     AKRVSKLKNANRFFVASNVHPQTLDVVRTRAETFGFEVIVDDAQKVLDHQDVFGVLLQ
                     QVGTTGEIHDYTALISELKSRKIVVSVAADIMALVLLTAPGKQGADIVFGSAQRFGVP
                     MGYGGPHAAFFAAKDEYKRSMPGRIIGVSKDAAGNTALRMAMQTREQHIRREKANSNI
                     CTSQVLLANIASLYAVYHGPVGLKRIANRIHRLTDILAAGLQQKGLKLRHAHYFDTLC
                     VEVADKAGVLTRAEAAEINLRSDILNAVGITLDETTTRENVMQLFSVLLGDNHGLEID
                     TLDKDVAHDSRSIQPAMLRDDEILTHPVFNRYHSETEMMRYMHSLERKDLALNQAMIP
                     LGSCTMKLNAAAEMIPITWTEFAELHPFCPPEQAEGYQQMIAQLADWLVKLTGYDAVC
                     MQPNSGAQGEYAGLLAIRHYHESRNEGHRDICLIPASAHGTNPASAHMAGMQVVVVAC
                     DKNGNIDLTDLRAKAEQAGDNLSCIMVTYPSTHGVYEETIREVCEVVHQFGGQVYLDG
                     ANMNAQVGITSPGFIGADVSHLNLHKTFCIPHGGGGPGMGPIGVKAHLAPFVPGHSVV
                     QIEGMLTRQGAVSAAPFGSASILPISWMYIRMMGAEGLKKASQVAILNANYIASRLQD
                     AFPVLYTGRDGRVAHECILDIRPLKEETGISELDIAKRLIDYGFHAPTMSFPVAGTLM
                     VEPTESESKVELDRFIDAMLAIRAEIDQVKAGVWPLEDNPLVNAPHIQSELVAEWAHP
                     YSREVAVFPAGVADKYWPTVKRLDDVYGDRNLFCSCVPISEYQ"
     gene            34816..35559
                     /locus_tag="OO357_001184"
     CDS             34816..35559
                     /locus_tag="OO357_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5886200.1"
                     /translation="MAIALVTGGSRGIGRATALLLAQEGYTVAVNYQQNLHAAQEVMN
                     LIMQAGGKAFVLQADISDENQVVAMFTAIDQHDEPLAALVNNAGILFTQCTVENLTAE
                     RINRVLSTNVTGYFLCCREAVKRMALKNGGSGGAIVNVSSVASRLGSPGEYVDYAASK
                     GAIDSLTTGLSLEVAAQGIRVNCVRPGFIYTEMHASGGEPGRVDRVKSNIPMQRGGQA
                     EEVAQAIVWLLSDKASYVTGSFIDLAGGK"
     gene            complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="OO357_001185"
     CDS             complement(35616..37049)
                     /gene="bglA"
                     /locus_tag="OO357_001185"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglA"
                     /protein_id="HCR5886201.1"
                     /translation="MKKLTLPKDFLWGGAVAAHQVEGGWNKGGKGPSICDVLTCGAHG
                     VPREITKEVLPGKYYPNHEAVDFYGHYKEDIKLFAEMGFKCFRTSIAWTRIFPKGDEA
                     QPNEEGLKFYDSLFDELLKYNIEPVITLSHFEMPLHLVQQYGSWTNRKFVDFFVRFAE
                     VVFERYKHKVKYWMTFNEINNQRNWRAPLFGYCCSGVVYTEHDNPEETMYQVLHHQFV
                     ASALAVKAARRINPEMKVGCMLAMVPLYPYSCNPDDVMFAQESMRERYVFTDVQLCGY
                     YPSYVLNEWERRGFNIKMEDGDLDVLREGTCDYLGFSYYMTNAVKAEGGTGDAISGFE
                     GSVPNPYVKASDWGWQIDPVGLRYALCELYERYQRPLFIVENGFGAYDKVEEDGSIND
                     DYRIDYLRAHIEEMKKAVTYDGVDLMGYTPWGCIDCVSFTTGQYSKRYGFIYVNKHDD
                     GTGDMSRSRKKSFNWYKEVIASNGEKL"
     gene            37094..37404
                     /locus_tag="OO357_001186"
                     /pseudo
     CDS             37094..37404
                     /locus_tag="OO357_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
     gene            37568..38227
                     /locus_tag="OO357_001187"
     CDS             37568..38227
                     /locus_tag="OO357_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HCR5886202.1"
                     /translation="MVQKPLIKQGYSLAEEIANSVSHGIGLVFGIVGLVLLLVQAVDL
                     NASATAITSYSLYGGSMILLFLASTLYHAIPHQRAKMWLKKFDHCAIYLLIAGTYTPF
                     LLVGLDSPLARGLMIVIWSLALLGILFKLTIAHRFKILSLVTYLAMGWLSLVVIYEMA
                     VKLAAGSVTLLAVGGVVYSLGVIFYVCKRIPYNHAIWHGFVLGGSVCHFLAIYLYIGQ
                     A"
     gene            complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="OO357_001188"
     CDS             complement(38423..39403)
                     /gene="ygfZ"
                     /locus_tag="OO357_001188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-modifying protein YgfZ"
                     /protein_id="HCR5886203.1"
                     /translation="MAFTPFPPRQPTASARLPLTLMTLDDWALATITGADSEKYMQGQ
                     VTADVSQMAEDQHLLAAHCDAKGKMWSNLRLFRDGDGFAWIERRSVREPQLTELKKYA
                     VFSKVTIAPDDERVLLGVAGFQARAALANLFSELPSKEKQVVKEGATTLLWFEHPAER
                     FLIVTDEATANMLTDKLRGEAELNNSQQWLALNIEAGFPVIDAANSGQFIPQATNLQA
                     LGGISFKKGCYTGQEMVARAKFRGANKRALWLLAGSASRLPEAGEDLELKMGENWRRT
                     GTVLAAVKLEDGQVVVQVVMNNDMEPDSIFRVRDDANTLHIEPLPYSLEE"
     gene            39646..39912
                     /gene="sdhE"
                     /locus_tag="OO357_001189"
     CDS             39646..39912
                     /gene="sdhE"
                     /locus_tag="OO357_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000354044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD assembly factor SdhE"
                     /protein_id="HCR5886204.1"
                     /translation="MDINNKARIHWACRRGMRELDISIMSFFEHEYDSLSDDEKRIFI
                     RLLECDDPDLFNWLMNHGKPADAELEIMVRLIQTRNRERGPVAI"
     gene            39893..40300
                     /locus_tag="OO357_001190"
     CDS             39893..40300
                     /locus_tag="OO357_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YgfX"
                     /protein_id="HCR5886205.1"
                     /translation="MVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMV
                     LLSLVVFDCVRSQRCINARQGEIRLLMDGRLRWQGQEWSIVKAPWMIKSGMMLRLRSD
                     GGKRQHLWLAADSMDEAEWRDLRRILLQQETQR"
     gene            complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="OO357_001191"
     CDS             complement(40340..40861)
                     /gene="fldB"
                     /locus_tag="OO357_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin FldB"
                     /protein_id="HCR5886206.1"
                     /translation="MNMGLFYGSSTCYTEMAAEKIRDIIGPELVTLHNLKDDSPKLME
                     QYDVLILGIPTWDFGEIQEDWEAVWDQLDDLNLEGKIIALYGLGDQLGYGEWFLDALG
                     MLHDKLSTKGVKFVGYWPTEGYEFTSPKPVIADGQLFVGLALDETNQYDLSDERIQSW
                     CEQILNEMAEHYA"
     gene            40973..41869
                     /gene="xerD"
                     /locus_tag="OO357_001192"
     CDS             40973..41869
                     /gene="xerD"
                     /locus_tag="OO357_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HCR5886207.1"
                     /translation="MKQELARIEQFLDALWLEKNLAENTLNAYRRDLSMMVEWLHHRG
                     LTLATAQSDDLQALLAERLEGGYKATSSARLLSAVRRLFQYLYREKFREDDPSAHLAS
                     PKLPQRLPKDLSEAQVERLLQAPLIDQPLELRDKAMLEVLYATGLRVSELVGLTMSDI
                     SLRQGVVRVIGKGNKERLVPLGEEAVYWLETYLEHGRPWLLNGVSIDVLFPSQRAQQM
                     TRQTFWHRIKHYAVLAGIDSEKLSPHVLRHAFATHLLNHGADLRVVQMLLGHSDLSTT
                     QIYTHVATERLRQLHQQHHPRA"
     gene            41894..42604
                     /gene="dsbC"
                     /locus_tag="OO357_001193"
     CDS             41894..42604
                     /gene="dsbC"
                     /locus_tag="OO357_001193"
                     /EC_number="5.3.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional protein-disulfide
                     isomerase/oxidoreductase DsbC"
                     /protein_id="HCR5886208.1"
                     /translation="MKKGFMLFTLLAAFSGFAQADDAAIQQTLAKMGIKSSDIQPAPV
                     AGMKTVLTNSGVLYITDDGKHIIQGPMYDVSGTAPVNVTNKMLLKQLNALEKEMIVYK
                     APQEKHVITVFTDITCGYCHKLHEQMADYNALGITVRYLAFPRQGLDSDAEKEMKAIW
                     CAKDKNKAFDDVMAGKSVAPASCDVDIADHYALGVQLGVSGTPAVVLSNGTLVPGYQP
                     PKDMKEFLDEHQKMTSGK"
     gene            42610..44343
                     /gene="recJ"
                     /locus_tag="OO357_001194"
     CDS             42610..44343
                     /gene="recJ"
                     /locus_tag="OO357_001194"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HCR5886209.1"
                     /translation="MKQQIQLRRREVDETADLPAELPPLLRRLYASRGVRSAQELERS
                     VKGMLPWQQLSGVEKAVEILYNAFREGTRIIVVGDFDADGATSTALSVLAMRSLGCSN
                     IDYLVPNRFEDGYGLSPEVVDQAHARGAQLIVTVDNGISSHAGVEHARSLGIPVIVTD
                     HHLPGDTLPAAEAIINPNLRDCNFPSKSLAGVGVAFYLMLALRTFLRDQGWFDERGIA
                     IPNLAELLDLVALGTVADVVPLDANNRILTWQGMSRIRAGKCRPGIKALLEVANRDAQ
                     KLAASDLGFALGPRLNAAGRLDDMSVGVALLLCDNIGEARVLANELDALNQTRKEIEQ
                     GMQVEALTLCEKLERSRDTLPGGLAMYHPEWHQGVVGILASRIKERFHRPVIAFAPAG
                     DGTLKGSGRSIQGLHMRDALERLDTLYPGMMLKFGGHAMAAGLSLEEDKFELFQQRFG
                     ELVTEWLAPSLLQGEVVSDGPLSPAEMTMEVAQLLRDAGPWGQMFPEPLFDGHFRLLQ
                     QRLVGERHLKVMVEPVGGGPLLDGIAFNVDTALWPDNGVREVQLAYKLDINEFRGNRS
                     LQIIIDNIWPI"
     gene            44434..45532
                     /gene="prfB"
                     /locus_tag="OO357_001195"
     CDS             join(44434..44508,44510..45532)
                     /gene="prfB"
                     /locus_tag="OO357_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311790.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HCR5886210.1"
                     /translation="MFEINPVNNRIQDLTERSDVLRGYLDYDAKKERLEEVNAELEQP
                     DVWNEPERAQALGKERSSLEAVVDTLDQMKQGLEDVSGLLELAVEADDEETFNEAVAE
                     LDALEEKLAQLEFRRMFSGEYDSADCYLDIQAGSGGTEAQDWASMLERMYLRWAESRG
                     FKTEIIEESEGEVAGIKSVTIKISGDYAYGWLRTETGVHRLVRKSPFDSGGRRHTSFS
                     SAFVYPEVDDDIDIEINPADLRIDVYRASGAGGQHVNRTESAVRITHIPTGIVTQCQN
                     DRSQHKNKDQAMKQMKAKLYELEMQKKNAEKQAMEDNKSDIGWGSQIRSYVLDDSRIK
                     DLRTGVETRNTQAVLDGSLDQFIEASLKAGL"
     gene            45542..47059
                     /gene="lysS"
                     /locus_tag="OO357_001196"
     CDS             45542..47059
                     /gene="lysS"
                     /locus_tag="OO357_001196"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001740376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HCR5886211.1"
                     /translation="MSEQHAQGADAVVDLNNELKTRREKLANLREQGIAFPNDFRRDH
                     TSDQLHAEFDGKENEELEALNIEVAVAGRMMTRRIMGKASFVTLQDVGGRIQLYVARD
                     DLPEGVYNEQFKKWDLGDILGAKGKLFKTKTGELSIHCTELRLLTKALRPLPDKFHGL
                     QDQEARYRQRYLDLISNDESRNTFKVRSQILSGIRQFMVNRGFMEVETPMMQVIPGGA
                     AARPFITHHNALDLDMYLRIAPELYLKRLVVGGFERVFEINRNFRNEGISVRHNPEFT
                     MMELYMAYADYKDLIELTESLFRTLAQDILGKTEVTYGDVTLDFGKPFEKLTMREAIK
                     KYRPETDMADLDNFDSAKAIAESIGIHVEKSWGLGRIVTEIFEEVAEAHLIQPTFITE
                     YPAEVSPLARRNDINPEITDRFEFFIGGREIGNGFSELNDAEDQAQRFLDQVAAKDAG
                     DDEAMFYDEDYVTALEHGLPPTAGLGIGIDRMVMLFTNSHTIRDVILFPAMRPVK"
     gene            complement(47102..47650)
                     /gene="idi"
                     /locus_tag="OO357_001197"
     CDS             complement(47102..47650)
                     /gene="idi"
                     /locus_tag="OO357_001197"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HCR5886212.1"
                     /translation="MQTEHVILLNAQGVPTGTLEKYAAHTADTLLHLAFSSWLFNAKG
                     QLLVTRRALSKKAWPGVWTNSVCGHPQLGESSEDAVIRRCRYELGVEITPPESIYPDF
                     RYRATDPRGIVENEVCPVFAARTTSALQINDDEVMDYQWCDLADVLRGIDATPWAFSP
                     WMVMQATNREARIRLSAFTQLK"
     gene            47705..47776
                     /locus_tag="OO357_001198"
     CDS             47705..47776
                     /locus_tag="OO357_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886213.1"
                     /translation="MINQESVYRQPPVLSGCRQVKTI"
     gene            complement(47773..47898)
                     /locus_tag="OO357_001199"
     CDS             complement(47773..47898)
                     /locus_tag="OO357_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886214.1"
                     /translation="MNFLMRAIFSLLLLFTLSIPVISDCVAMAIESRFKYMMLLF"
     gene            complement(47900..49348)
                     /gene="uacT"
                     /locus_tag="OO357_001200"
     CDS             complement(47900..49348)
                     /gene="uacT"
                     /locus_tag="OO357_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urate/proton symporter UacT"
                     /protein_id="HCR5886215.1"
                     /translation="MSAIDSQLPSSSGQDRPTDEVDRILSPGKLIILGLQHVLVMYAG
                     AVAVPLMIGDRLGLSKDAIAMLISSDLFCCGIVTLLQCIGIGRFMGIRLPVIMSVTFA
                     AVTPMIAIGMNPDIGLLGIFGATIAAGFITTLLAPLIGRLMPLFPPLVTGVVITSIGL
                     SIIQVGIDWAAGGKGNPQYGNPVYLGISFAVLIFILLITRYAKGFMSNVAVLLGIVFG
                     FLLSWMMNEVNLSGLHDASWFAIVTPMSFGMPIFDPVSILTMTAVLIIVFIESMGMFL
                     ALGEIVGRKLSSHDIICGLRVDGVGTMIGGTFNSFPHTSFSQNVGLVSVMRVHSRWVC
                     ISSGIILILFGMVPKMAVLVASIPQFVLGGAGLVMFGMVLATGIRILSRCNYTTNRYN
                     LYIVAISLGVGMTPTLSHDFFSKLPAVLQPLLHSGIMLATLSAVVLNVFFNGYQHHAD
                     LVKESVSDKDLEVRTVRMWLLMRKLKKNEHGE"
BASE COUNT        11958 a        12401 c        13344 g        11821 t
ORIGIN      
        1 tttgaactgc cagaaattac cgataatgaa ctgttagtga gtgtaatttc tgacagcgtc
       61 tgtttatcga cctggaaagc ggcgttactc ggtagtgaac ataaacgcgt acccgacgat
      121 ttagaaaatc atccggtcat taccgggcat gaatgtgccg gggttattgt cgaagtgggt
      181 aaaaatctca ctggcaaata taaaaaaggc cagcgttttg tattgcaacc ggcgatgggg
      241 ttaccaagcg gatattcagc gggctacagc tacgaatatt ttggcggcaa tgccacttat
      301 atgattattc ccgaaatcgc cattaatttg ggctgcgtat taccgtatca cggctcttat
      361 tttgctgcgg cgtcgctggc agagcctatg tgctgcatta ttggtgctta tcatgccaat
      421 tatcacacca cgcaatatgt ttatgagcat cgcatgggcg tcaaacctgg cggcaatatt
      481 gcactgctgg cgtgtgcggg tccgatgggc attggcgcta tcgattacgc cattaacggt
      541 ggcatacaac cgtcacgggt ggtggtggtc gatatcgacg acaaacgcct ggcgcaggtg
      601 cagaagctgc tgccggtgga tctggcggcc agtaaaggca ttgagctggt gtatgtgaat
      661 accaaaggga tgagcgatcc tatccagatg ctgcgggcgc tgacaggaga tgtcgggttc
      721 gatgacattt ttgtttatgc ggcggtgcct gctgtcgttg agatggctga cgaattactg
      781 gcggaagatg gctgtctgaa cttctttgcc gggctgacgg ataaaaactt caaagtgccg
      841 tttaatttct acaacgtcca ttacaacagc acgcacgtag tcggtacatc cggcggttca
      901 acggacgaca tgaaagaggc gattgccctt agcgccacag gacagttaca gccgtcgttt
      961 atggtgaccc atattggtgg cctggatgcg gtgccagaaa ccgtgctcaa tctgccggat
     1021 atccctggcg gtaaaaaact catttataac ggcgtgacca tgccgctcac tgccattgcc
     1081 gattttgccg aaaaaggcaa aaccgatccg ctgtttaaag agttggcgcg gctggttgag
     1141 gaaacgcacg gcatctggaa tgaacaggcc gagaaatatc tgctggcaca atttggcgtt
     1201 gatatcgggg aggccgcgca atgatgtccc tggcgtggcc attatttcgc gttacggaac
     1261 aggcagcgtt ggctgcctgg ccgcaaaccg gatgtggcga caaaaataaa attgatggcc
     1321 tggcggtcac cgcgatgcgc caggcattaa acgacgtcgc ttttcgtgga cgagtggtta
     1381 tcggcgaagg agaaattgac catgcgccga tgctgtggat tggcgaagag gtgggcaaag
     1441 gcgatgggcc agaagtcgat atcgcggttg atcccattga aggcacgcgg atggtggcga
     1501 tggggcagag caatgcgctg gcggtcatgg ctttcgctcc tcgagatagc ctgttacatg
     1561 cgcccgatat gtatatgaaa aagctggttg ttaatcgact ggctgccggg gcgattgatc
     1621 tgtctttgcc tctggcggac aacttgcgta acgtggcgcg ggcgttaggt aaaccgctgg
     1681 acaagctgcg catggttacg ctggataaac cgcgtctgag tgccgcgatt gaagaagcaa
     1741 cacagctggg cgtgaaggtt tttgccctgc cggatggcga tgtcgccgcc agcgtgctga
     1801 cctgctggca ggataatcct tacgatgtga tgtacaccat cggcggtgcg ccagaaggcg
     1861 tgatttctgc ctgtgccgtt aaagcgttag gcggggatat gcaggccgag ctgattgact
     1921 tttgtcaggc taaaggggat tacacggaaa atcggcagat tgcagagcag gagcgcaagc
     1981 gttgtaaggc aatgggcgtt gacgttaacc gtgtgtactc gctcgatgaa ctggtgaggg
     2041 ggaacgatat cctctttagc gctacgggcg tgacgggcgg cgagttggtg aacggtatcc
     2101 aacagacggc gaatggggtg cggacgcaga cattactgat cggcggcgcg gaccaaacgt
     2161 gtaatataat agactccctg cattgatggt gatctatcgg aagaccaatg gcgacgctga
     2221 cagaagatga tgtgcttgag caactggatg cacaggacaa tttattttca tttatgaaaa
     2281 ctgcgcattc tattttgctc caggggatac gccagtttct gccgtcgctg tttgtcgata
     2341 acgatgaaga gatcgtcgaa tatgcagtga agccgttact cgcccaaagc ggcccgcttg
     2401 acgatattga tgttgcgctg cgtttgattt atgcgctggg gaaaatggat aaatggctgt
     2461 atgccgatat cacgcacttt tcccagtact ggcattacct gaacgaacag gatgaaacgc
     2521 ccggatttgc cgatgatata acctgggatt ttatcagcaa tgtcaacagt ataactcgca
     2581 atgcgacgct ctacgatgcg ttaaaagcga tgaagttcgc cgatttcgcc gtctggtcag
     2641 aggcgcgttt tagcggaatg gtcaaaacgg cgctgacgct ggcagtaacg accaccttaa
     2701 aggaattaac gccgtgaaaa ttgaattaac ggtgaatggg ctgaacgtgc aagcgcagta
     2761 ccatgatgaa gagatagaac gcgtgcataa actgttgctg cgtatgctgg cggccttaca
     2821 gacggtaaat ccgcagcggc gtacggtggt ttttctttgc gctccgccgg ggacgggtaa
     2881 atctacccta accaccttct gggaatatct cgcgcaacaa gacccggagc tgcccgctat
     2941 tcaggcgctc ccgatggatg gttttcacca ttacaatagc tggctggacg cgcatcaatt
     3001 gcgccccttc aaaggcgcac cagagacatt tgacgttgcg aaactggcgg aaaatctgcg
     3061 ccaggtggtg gaaggggatt gtacgtggcc gcagtacgat cgacaaaagc atgatcctgt
     3121 tgaagatgcg ttgcacgtta ccgcaccact cgtcatcgtc gaaggaaact ggttgttgct
     3181 ggacgacgag aagtggtgcc agcttgcgca attttgtgat ttctcgatct ttatcaacgc
     3241 gccagctgct gcgttgcggg agcgtctggt ggggcgcaag ctggcgggag gggtatcgct
     3301 ggcagatgca gaagcctttt atgaccgtac cgacgggccg aatgttcgcc gggggctgga
     3361 agagagtctg cctgcaaatt tgaccttaat gatgaccgct acgggtgaat atcgtctggt
     3421 ggattaacgt tcctgattac accatcttcc agccgcacca cacgagagaa atgacgccgg
     3481 gtaaatgcgg cgtcatgact gattgctacc acgctcgtgc ctcgctggcg gcacttctcc
     3541 agccagcttt caaacacgct caaccaattt tcgtcaaaat cccgactcgg ctcgtccagc
     3601 agtaatagtg gcggagagag tgcttccagg caggcgacgg cgaccatccg tcgctgcgca
     3661 ttatgcagat ccaacgggtg cgagttggca gtgtctgcta actgacaaca ttgcagggca
     3721 gcatgggtac gttgagtgat ttcatccgcc gggcattttt gcagtttcaa accaaatgcg
     3781 atctcgtcag cgacggtgct gtgaaaaagt tggttttccg cttcctgaaa cagtacgcca
     3841 acctttgccg cgcgctggcg gtttttcagg tttttcatcg cctgttgttg cagcataacc
     3901 gtaccggctg tgggcgtaag aagacctgcc attactcgta acagcgtcga tttccctgcg
     3961 ccattgtcac cggtcagcgc caaccattca ccttgtttga gttgcagaga aatgtcgcaa
     4021 agacaatccg ttgccgcgcc aggccagcga taagatatct tatttaacgt taacatagtg
     4081 gctggagcgc tccgttttgc aattgccacg cttgctggca gtagttcagg aaaggtgtgc
     4141 gatgacgttc aaaaagaata atcagcgaac cacgttcaaa cgcccagtgt tgtaagcgct
     4201 gcaacagcat ttcgctggcc tgcggtgtca ggcggctaaa ggcttcatca agaattaaga
     4261 gctttggctg catcgcgatg gcgcaggcga tgatcacgcg ctgtgtctct ccaccggaga
     4321 gtgttgccgg gtgacgatgg cgcaacggct ggcactcggt cagagtcagg gcggcatcaa
     4381 tccgcgccat aatctctgct tcagcaagac acagattttc tggcccaaag gcaacttctt
     4441 cttccacgct aaaggtacag ccggagagct gcaaataggg cgattgctgg acgagctgga
     4501 tggtggcgga ttgttcatta agcgggagat gcccaatggg cgtaccgagg agcgtccccg
     4561 tgcctgttat ctctcttggc agaaagtccg gataccagcc cgccatcagc tgtgccagcg
     4621 tacttttgcc actgccgttg tcaccaaaga tggcgaccat ccccggtttg acatagtgaa
     4681 agtcataaca gaacggagga tgcgttgagt gtgtcgggca ataacggaac tgttctaacg
     4741 taaccatatc cacgctccga attctgtcag catcagcaag atcatggtgt aacgcgctac
     4801 gcgttgtagg gtgctatcgg ctggtgccca caacgttgtt cgtcggttgt ggatccgaaa
     4861 tgcccgcata tccagcgccg caccacggac agccagatcg ttaagcgcat tgtgtgttag
     4921 cggaataatt aacgcgggca tagcacgtaa acgctgatac cagccctcat ccagcggcac
     4981 gccgcgggca cgctgcgctt catgaataat cgctaattgc cgttttaact gctcaacgac
     5041 caataacggc ccggcaaaca gataagcgac gcctggcggc aggcgagaag caaacagagc
     5101 gcgaataaaa cattgcacgg ggacgtactg cattcacaac tgtgaggttg aaacaatcgc
     5161 cagaatcagc aaccagagcg taatagcatg cgaccaccgt tctggcgagc gtggcgtgcc
     5221 gcttagccat tctggtagcc agccaccgtg taccagccac agcccggctc ccagcgagaa
     5281 catcagccag acgacatatt ttgcccgccg ccgcgtggcc tttagcgcaa taaggcagaa
     5341 aaaggtcgcc gcgctataga tcggcaaaat ggtctgcgcg ggcaggataa gcgtggtgca
     5401 ggccgctaat gcccacagtg ttaatgaggt aaatggatgc atttagcgca cggcagccat
     5461 tgccggaaaa tggcgcgtgg tgcgaatcgg cagctggcgc agtaaaagcc agacaatcac
     5521 cgctgtaaga attttatcga ccaggttcgc gcctatcacc gtaatcgcca ccgactccac
     5581 caggttttgc cccatcgagt gcatccaggc aacgaacaaa tcggctccgc taccggtgac
     5641 accgccgaac agcgcagtac gcagcggtac agcgacgacc gttaccgcaa gcgtaataat
     5701 cacgccgctg acaacgactt ttggcagggt gcgaaaccaa cccgctcgcg ccagccaacc
     5761 ggagaccagg ccgatgacca tcgcgacggg tgcaaatgcg gcggcgatcg ggtcggtcag
     5821 caatccccag agtaaattgg tcagtaagcc ggtcagcatt ccgatcaccg gaccaagcag
     5881 taccgcgcta ataagtgtgc cgatggagtc gagaaatata ggtaatttga ccatgctggc
     5941 cagttgcccg ccgatcatat tgatggcgat agatataacg atcaacacca gtgcctgact
     6001 ggaaaagtgg ctacgcgcca taagaaaacc tctttgaaag tagttattta acgggggaaa
     6061 tatgcaactc gtcgtaacca gaaagcgcgc aaggttggcg gctaaacgcc agttcctcaa
     6121 ggctaccgat caccagttca agcgtggtcc gcacggtaca gccgggcatc atatgaccgc
     6181 caagcatcgt gccgtgtgga tcggaaacgc agagatgaag atgctcacca ctctgttcca
     6241 gcgtgccgtt tagtgagatc acttcgaatg taccgttcag gagcgtggtg ccttcttgcc
     6301 cggcatagcg caaggcgacg tcagtcaggc taccggtaca gcctgcaatc taggcggcat
     6361 gaagttgatg ttgctgtgca aaagcgtgta gctgggaaaa aacttcctgc ccggggagga
     6421 ggcgcagtgc gtaaaagcgt gcggaggaag aatatgagta gggcaatgtc atcattggag
     6481 tactccgaaa ctcggacgta ttgcggataa tatcgacgct gcatcagtca tattctgata
     6541 taagtcaggg tttgtgacgg cacaacgaaa agattttgct acgcttattc gtgattctgc
     6601 aaagtggaat aaattccaca ggggcggggc tgaaaacgtg acgtaagtca gataattgct
     6661 ttctttgact ggacaatcat tccttttatt ccacgtttcg cttatcctag ctgaagcgtt
     6721 tcagtcgatt aaatgttcga caattaacca atcagtcgca gtttgcgaca ggtaaggttt
     6781 ccccggacga tttgctggat tactctgtct ggcaaatttg ctcagggaaa accttgcagg
     6841 agatctatga ccgtacgcgt agcgataaat ggcttcggtc gcatcgggcg taatgtggtt
     6901 cgtgctttgt atgaatccgg acgccgggcg gaaattaccg tggtggcaat caacgaactg
     6961 gcggatgctg cgggcatggc gcatttgttg aaatatgaca ccagccatgg ccgttttgca
     7021 tgggaagtac gacaggaacg cgatcaactt tttgttggtg atgacgccat ccgcgtattg
     7081 catgaacgtt cactgcaatc gctcccctgg cgtgaacttg gcgttgatgt agtcctcgac
     7141 tgcaccggcg tatatggctc ccgcgagcat ggcgaagcgc atattgccgc cggggccaaa
     7201 aaagtgctct tttcacatcc tggcagtaac gatctcgacg cgaccgttgt ttacggcgtc
     7261 aatcaggatc aacttcgtgc ggaacaccgc atcgtttcta acgcttcctg taccacgaat
     7321 tgcataattc ccgtcatcaa attgttagat gatgcgtacg gtattgagtc cggcactgtg
     7381 accacaattc actccgccat gcacgatcaa caggttattg atgcatacca tcctgacctg
     7441 cgtcgcaccc gggcagccag ccagtcgatc attccggtcg atactaaact ggccgctggt
     7501 atcacacgat tttttccgca atttaacgat cgctttgaag cgattgcggt acgtgtgcca
     7561 accataaatg tgacggcaat cgatttaagc gtgacggtga agaaacctgt aaaagccaat
     7621 gaagtcaacc tgttgctgca aaaagcagca caaggtgcat ttcatggtat agttgactat
     7681 acggaattgc cgttggtctc tgtagatttt aaccacgatc cgcacagtgc cattgtcgat
     7741 ggcacccaaa cccgggtcag tggcgcacac ctgatcaaaa cgttggtctg gtgcgataac
     7801 gaatggggct ttgctaaccg aatgctcgac acgacgttag ctatggctac tgttgctttc
     7861 aggtaagacg caagcagcgt ctgcaaaact tttagaatca acgagaggat tcaccatgtc
     7921 tgtaattaag atgaccgatc tggatcttgc tgggaaacgt gtatttatcc gtgcggatct
     7981 gaacgtacca gtaaaagacg ggaaagtaac cagcgacgcg cgtatccgtg cttctctgcc
     8041 gaccatcgaa ctggccctga aacaaggcgc aaaagtgatg gtaacttccc acctgggtcg
     8101 tcctaccgaa ggcgagtaca acgaagaatt ctctctgctg ccggttgtta actacctgaa
     8161 agacaaactg tctaacccgg ttcgtctggt taaagattac ctcgacggcg ttgacgttgc
     8221 tgaaggtgaa ctggttgttc tggaaaacgt tcgcttcaac aaaggcgaga agaaagacga
     8281 cgaaaccctg tccaaaaaat acgctgcact gtgtgacgtg ttcgtaatgg acgcattcgg
     8341 tactgctcac cgcgcgcagg cttctactca cggtatcggt aaattcgctg acgttgcgtg
     8401 cgcaggcccg ctgctggcag ctgaactgga cgcgctgggt aaagcactga aagaacctgc
     8461 tcgcccgatg gtggctatcg ttggtggttc taaagtatct accaaactga ccgttctgga
     8521 ctccctgtct aaaatcgctg accagctgat tgttggtggt ggtatcgcta acacctttat
     8581 cgcggcacaa ggccacgatg tgggtaaatc cctgtacgaa gctgacctgg ttgacgaagc
     8641 taaacgtctg ctgaccacct gcaacatccc ggttccgtct gatgttcgcg tagcaaccga
     8701 gttctctgaa actgcaccgg ctaccctgaa atctgttaac gatgtgaaag ctgacgagca
     8761 gatcctggat atcggtgatg cttccgctca ggaactggct gaaatcctga agaatgcgaa
     8821 aaccattctg tggaacggtc cggttggcgt gttcgaattc ccgaacttcc gcaaaggtac
     8881 tgaaatcgtg gctaacgcta tcgcagacag cgaagcgttc tccatcgctg gcggcggcga
     8941 cactctggca gcaatcgacc tgttcggcat tgctgacaaa atctcctaca tctccactgg
     9001 cggcggcgca ttcctcgaat tcgtggaagg taaagtactg cctgcagtag cgatgctcga
     9061 agagcgcgct aagaagtaaa aaatcacagg gcagggaaac ctgcacttgt ttcagcgcgc
     9121 ttttagagca cgcatttttt catactctaa ataattcgag ttgcaggaag gcgacaagag
     9181 agtgaatccc caggagctta cataagtaag tgactggggt gagcgaacgc agacgcagca
     9241 catgcaactt gaagtattat gagtataagg cccgacgata caggacaaga gacatgtcta
     9301 agatttttga tttcgtaaaa cctggcgtaa tcactggtga tgacgtacag aaagttttcc
     9361 aggtagcaaa agaaaacaac ttcgcactgc cagcagtaaa ctgcgtcggt actgactcca
     9421 tcaacgccgt actggaaact gctgctaaag ttaaagcgcc ggttatcgtt cagttctcca
     9481 acggtggtgc ttcctttatc gctggtaaag gcgtgaaatc tgacgttccg cagggtgctg
     9541 ctatcctggg cgcgatctct ggtgcgcatc acgttcacca gatggctgaa cattatggtg
     9601 ttccggttat cctgcacact gaccactgcg cgaagaaact gctgccgtgg atcgacggtc
     9661 tgctggacgc gggtgaaaaa cacttcgcag ctaccggtaa gccgctgttc tcttctcaca
     9721 tgatcgacct gtctgaagaa tctctgcaag agaacatcga aatctgctct aaatacctgg
     9781 agcgtatgtc caaaatcggc atgactctgg aaatcgaact gggttgcacc ggtggtgaag
     9841 aagacggcgt ggacaacagc cacatggacg cttctgcact gtacacccag ccggaagacg
     9901 ttgattacgc atacaccgag ctgagcaaaa tcagcccgcg tttcaccatc gctgcgtcct
     9961 tcggtaacgt acacggtgtt tacaagccgg gtaacgtggt tctgactccg accatcctgc
    10021 gtgattctca ggaatatgtt tctaagaaac acaacctgcc gcacaacagc ctgaacttcg
    10081 tattccacgg tggttccggt tctactgctc aggaaatcaa agactccgta agctacggcg
    10141 tagtgaaaat gaacatcgat accgataccc aatgggcaac ctgggaaggc gttctgaact
    10201 actacaaagc gaacgaagct tatctgcagg gtcagctggg taacccgaaa ggcgaagatc
    10261 agccgaacaa gaaatactac gatccgcgcg tatggctgcg tgccggtcag acttcgatga
    10321 tcgctcgtct ggaaaaagca ttccaggaac tgaacgcgat cgacgttctg taagtcattc
    10381 ctttctgctt atctcaaggc ccgctctgcg ggtctttttt tcgccaaaag caaggtgatt
    10441 cagatgagaa atctgtgatc tatttggcaa aattatgctt tattgtttac ccttgtcaga
    10501 ctgcccgtca taaggcggcg gagtgtattt ctccattttg agtcagttga aaaggaatat
    10561 tgaatggaag atttgaatgt tgtcgatagc ataaacggcg cgggaagctg gctggtagct
    10621 aaccaggcgc tgctgctaag ttatgcagta aacatcgtgg cggcactcgc gatcatcatc
    10681 gttggtttga ttatcgcgcg gatgatttcc aacgcggtga atcgcctgat gatctcccgt
    10741 aaaatcgatg ccactgttgc tgattttctt tctgcattag tccgttacgg tattatcgcc
    10801 tttacgctaa tcgctgccct gggacgcgtg ggtgtacaaa cagcgtcagt cattgctgta
    10861 ctcggtgccg caggcttagc tgttggtctg gctttgcagg ggtcactttc taacctggcc
    10921 gctggcgtgt tacttgtcat gttccgcccg ttccgtgccg gagaatatgt tgacctcggc
    10981 ggcgtagccg gtactgtgct gagtgtgcag attttctcca ccaccatgcg tactgcagac
    11041 ggtaaaatta tcgttattcc gaacggtaaa attattgccg gaaatattat taacttctcc
    11101 cgcgagccag ttcgccgtaa cgaatttatt attggcgtgg cgtatgattc cgatatcgat
    11161 caggttaagc agatcctgac caatattatc cagtctgaag atcgcatttt gaaagatcgc
    11221 gaaatgactg tgcgcctgaa cgaacttggt gcatcgtcga ttaatttcgt ggtccgcgtc
    11281 tggagcaaca gcggcgatct gcaaaacgtg tactgggatg tgctggagcg tattaaacgt
    11341 gaatttgatg ccgccggtat cagcttcccg tacccgcaaa tggatgtgaa ctttaagcgg
    11401 gtgaaagaag acaaagctgc gtaatcaacg ctgaggccag ataatactct ctggcctctc
    11461 tcttattagt tattctgatt gccaattaat attatcaatt tccgctaata acaatcccgc
    11521 gatatagtct ctgcatcaga tacttaattc ggaatatcca acgtgttttc ttattacttt
    11581 caaggtcttg ctcttggggc agctatgatt ctgccgctcg gcccacaaaa tgcttttgtg
    11641 atgaatcagg gcattcgtcg tcagtaccac attatgattg ccttactttg cgctatcagc
    11701 gatttggtcc tgatttgcgc ggggattttt ggtggtagcg cgttattgat gcagtcgccg
    11761 tggttgctag cgctggtcac ctggggcggc gtagtcttct tgctctggta tggttttggc
    11821 gcttttaaaa cagcgatgag tagtaatatt gagttagcta gtgccgaagt cctgaagcaa
    11881 ggcagatgga aaattatcgc caccatgttg gcagtgacct ggctgaatcc gcatgtttac
    11941 ctggatactt ttgttgtact gggcagcctt ggcgggcagc ttgacgtcga accaaaacgt
    12001 tggtttgcgc tcgggacaat tagcgcctct ttcctgtggt tctttggtct ggctattctc
    12061 gcagcctggc tggcaccgcg cctgcgcacg gcaaaatcac agcgcattat caatctggta
    12121 gtgggatgtg tcatgtggtt tatagccttg cagctggcga gagacggtat tgctcatgca
    12181 caagccttgt tcagttaggc gctatctgat ggaaaaataa aacagaggcg ctaagcttgc
    12241 ctccagaggt cctgaatttt gggcaagtca attaacacgg agagactaac gtgaagttca
    12301 aagttatcgc cctggcggca ttaatgggta ttagcgggat ggcagcgcag gctaacgaat
    12361 tgccggatgg accgcatatt gtcacctccg gtacggcaag tgtggatgcg gtgccagaca
    12421 ttgccactct tgcgattgaa gttaacgtgg ccgcgaagga tgccgctaca gccaagaaac
    12481 aggcagatga gcgcgtcgca caatacattt ccttccttga actcaatcag atcgcgaaaa
    12541 aagatatcag ctcagcgaat ttacgcaccc agccagatta tgactatcag gatggtaaaa
    12601 gtatcctgaa aggctaccga gctgtgagaa cggtggaagt cacgctccgt cagttagaca
    12661 aactgaattc cttgctggat ggcgcgctga aggcgggtct taacgaaatt cgttctgtgt
    12721 cgctgggcgt ggcgcagccg gatgcctata aagacaaagc gcgtaaggca gcgattgata
    12781 acgcgattca tcaggcgcag gaactggcga acggctttca tcgtaaactg gggccggtat
    12841 atagcgtgcg ctaccatgtt tccaactatc agcccagccc aatggtgcgg atgatgaaag
    12901 ccgatgccgc gccggtgtct gcccaggaaa cttacgagca ggccgctatt cagtttgatg
    12961 atcaggtcga tgtggtcttc cagttagaac ctgtggatca acaacccgct aaaacacctg
    13021 cagcacaata atacttgtag cccgacgtat tcgtcgggct aatgccttta cccgatattg
    13081 cctccttttt ctgtaatttt ctgtaaaacc atgacgtaat cacctatctt tgctcgcggt
    13141 ttcatcaagg ataggctgac tgaaacggtt aagcggacaa ggatgcgggg aggggggatg
    13201 gacattttta tttcgaaaaa gatgcgcaat ttcattttat tagcgcaaac caataatatt
    13261 gcccgggcgg cagaaaaaat ccatatgacg gcttcgccat ttggtaaaag cattgccgcg
    13321 ctggaagaac aaattggcta tacgctattt acccgcaaag ataataatat cagcctcaat
    13381 aaagccggtc aggaactgta tcaaaaactg ttcccggttt atcaacgact ttctgcgatt
    13441 gataatgaaa tccataactc agggcgtcgt tcacgagaaa ttgtgattgg catagataat
    13501 acttatccga caattatttt cgatcagttg attagtctcg gagataagta tgaaggcgtg
    13561 accgcccagc cagttgagtt cagcgaaaac ggcgttatcg ataatctttt tgaccgtcag
    13621 ttagatttta ttatctcccc acagcatgta tctgcacgag ttcaggaact ggaaaacctg
    13681 actatcagtg aattaccacc gctgcgattg ggctttctcg tttctcgccg ttatgaagag
    13741 cgtcaagagc aagagctgct tcaggagctg ccctggctac aaatgcgctt tcaaaaccgg
    13801 gctaacttcg aagcaatgat tgatgcgaat atgcgacctt gtgggatcaa tcccaccatt
    13861 atttatcgtc cctacagttt tatggccaaa atcagcgccg tggagcgcgg acattttctg
    13921 acggtgatcc cgcattttgc ctggcgactg gtgaatccgg caacgctgaa atattttgat
    13981 gcgccgcaca ggccgatgta tatgcaggaa tatctttatt caatcagaaa tcatcgttat
    14041 accgccacga tcttcagcat attgctgaag atcgtgacgg gacaaaccat taacccagca
    14101 tcgagccggt tgcaattaaa ttacggtgta agtcgaagac gtggctaaga tcgtggtgaa
    14161 tatgtccgcc gggcgcattt tccagatagc gatgcagata atcccgatac ataggatgtg
    14221 cacaattatc aatgatagtg cgggcgcgtt gaagcgggga aagaccgcgc agatcggcga
    14281 tcccttgttc ggtgatgatc actttgacgc tgtgttcgct gtgatcaaca tggctgcaca
    14341 ttggcacgac ggttgagatc ttgccttctt tagcaatcga cggagccata aagatcgaca
    14401 gatacgcgtt gcgttcaaaa tcaccgctgc cgccgatgcc gttcatcaga tcgaccccgg
    14461 ctacgtgcgt tgaatttgca tgcccgtaaa tatcaaactc caggccgacg ttcagagcga
    14521 tgacgcccag acgacggatg atttccgggt tattggaaat ctcctgcgga cgcaacacaa
    14581 tgcggctggc aaagtaatcc atattgtcgt aaatcttgcg cagggaatcg gccgagattg
    14641 tcaggctgga ggcgctggcc ccgctgattt tgccggtttc cagtaaatgc accaccgatt
    14701 cctgtagcac ttccgaatac atcatgaacg gaggaatttc cgggttttcc cccagacgtg
    14761 ccattaccgc attattgata ttgcccacgc cactttgcag cggcagaaat tccggcggaa
    14821 tacgcccatg cgccatttcc tgcaataaga acgtgaccac gttatcggca atctgctggc
    14881 acatgggatt ttgcttatcc agcatattac cggcgtcggg caagttggtt tccacgacgg
    14941 cgacaatctt tttcggatcg atttgcacat agcgggtacc gacgcgatcc attgcatgga
    15001 agatcgacac gctattgcgc cgcggtggcg cgccaggaat cacaatatct gccagttctg
    15061 caacgcgcgg atcgtgatag tgattaagtt caatgatcac tttcttcgct cgcagcagct
    15121 aggtcggcgc attaccgatc ccgctggtta accagactcg accatccggt gccagtgccg
    15181 atgcttcaat gacggcaaca tcaatgtcgc cgaagaaacc gtaattgacc atttgcgcca
    15241 cttcgctcaa atgcaggtca acgaaactca ccgcgccctg attgatcttt ttacgtaaac
    15301 tggacgatgt ttgatatggc gcacgccagg aaacagcatc ggcgtcagaa agtacatcgt
    15361 cagcggcggc gctgattgac gcccccgtca gcaggcgaat ttgatacggc tttttggcct
    15421 catgctgttc gttagctctg cgggcaatcg cggcgggtag ggctttcggc gaacccgctg
    15481 gggtaaagcc gctaaatgcc accatgtcgt tatgctggat aatttctgcc gcttcatcgg
    15541 cggtcatcct tgtccactga gtttccattt ttactccttc gttcgcaggg attaatgacc
    15601 aacgaaatta ggtttacgtt tttcgaggaa agcgttcatc ccttcctggt aatcttcgct
    15661 gtcataaacc gcgcggcgca tcccctgaat acgttcaaat tcatcggagt tcatggtgtg
    15721 tgcttcgccc agtacacgca gctcttcttt gataacggca atggctaacg gcgctttctc
    15781 agagatgtgg tgcgccattt gtaaggtgaa atcttccagt tcttccactt ccacaacatg
    15841 gttgaggatg ccgacagcca gcgcgcgctg ggcggtgatt ggcgaagcgg taaaaatcag
    15901 ctctttgaca atgtggaagc ccgcgtcgcg ggtcaggttg tgaatgccga ccaggttata
    15961 cgggacgccg aggtttacag gcgtcattga gaaggttgag gtactggcgg cgatgatcag
    16021 atcggaactc atgatcattt caaatgcgcc accccaaaca ctaccttcca ccatcgaaat
    16081 gatcggtttc gggaattttt ggatcatgcg ggtgatttga cgcaatggat catcatagga
    16141 gagcggatcg cgaccgccag acggcagttc gtgaatatcg tgacctgcgg agaagacttt
    16201 ggatccactt ggcgcccgca aaatgataca gcgaatttcc ggccggttga gatcgcttaa
    16261 cgcctgcata agatcatcaa taaagacttt acttaaggca ttaagttttc gtccatagtt
    16321 aaactcaatg accgccactt tgttgatagt ggcaacgttc acatactgat aagacataaa
    16381 aattccttta atcaaaatat tgcgtctgga taaattcact gagctgccgc aggccggtgc
    16441 gcggtgagag cgtattgttt ttgaccgcta aaagcgtctg gcggtaatag cgatcgaaat
    16501 cttcattcgc gaacaggtga ttcagtactt cttcttcggt ctgcttacgc agccattcca
    16561 ccgattgttg ttgccgcact tgttgtaaac gaccactggc agttagcgcg gttttgaagt
    16621 cgatgatggc gtgccagatc tcatcgattc cacgtttttc cagtgcgcta caagtcagac
    16681 cccgtggctg ccattcgtcg tatttacgtc gcagaatatg cagggcactc tcgtacatat
    16741 gccgggcaat ggcgacattg gtatggttat cgccatcgtc tttgttgata acgagcagat
    16801 cagccacttc catcagccct tttttaatgc cctgcagatc atcgccgcca ccggcaattt
    16861 gcaacgagat aaaacagtcc accatgcggg cgacttccgt ttccgactgc gcgatgccaa
    16921 ccgtttcgac aatcactacg tcataacccg ccgcttcgca taacagcatt aattcccgtg
    16981 ctcgctgact ggcaccgccc agatgaccgg aggagggtac cgggcgaata aacgccgcct
    17041 cggcacgcgc caggtcattc atgcgggttt tatccccgag aatgctaccg ccagtgaccg
    17101 ggctgctggg atcgaccgca ataacagcaa cctttaatcc ctcacgaatc aacaacatgc
    17161 caaaggcttc aagaaaggta cttttccccg cgccgggtgt gccggtaacg cccagtcgca
    17221 gggcgttacc acagaacggc ataatagcgt caagcagctg tgtacttagc gcctgatgac
    17281 gtgggtgacg gctttccacc agcgtcatgg cttgggcgag cgtggcacgc tcaccctgac
    17341 gtaagcggcg aatactttct gccagcgtgg cttcattaat catgatgctg gcttatcaga
    17401 ttcagtacgt cgcgcacact gtcgagcata ggtgtacctg gaccataaat cgccgccacg
    17461 ccgcgctctt gcaggaaggc gtaatcctgc ggcggaatga cgccacccgc gaccacgcag
    17521 atatcttcgc gtccccattt tttcagcgct tcgaccagtt ccgggatcag cgttttatga
    17581 ccggcagcca gtgaggatgc gcccactacg tgaacgtcgt tttctacggc taggcgggcg
    17641 atctcttcag gtgtagagaa catcgggctt aaatctacgt cgaaaccgag atcggaatag
    17701 gcgctggcga tcactttcgc gccgcgatcg tgtccatcct ggcccatctt agcgatcaga
    17761 atgcgcgggc gacgaccatt gtcggcaagg aactgctccg tttgcgcaac aatggcatcg
    17821 aactcggagg ccgatttctc agactgatga tagctttgcg caatcacgcc ggtaacacac
    17881 tggcttggca ccagataacg gtcgaaagcg acttccagcg catcggaaat ttcacccagg
    17941 gtggcgcgaa cgcgagcggc attaacagcg gcagccagca ggttttcgtt atgctgtgcg
    18001 gcgtgagtca gggcgttcaa cgcggcggtt acggcggcat catcacgggt ggcgcgaatg
    18061 cgttccagcg aagcaatttg ctcgttacgc accatcacgt tgtcgatctc aagtacatcg
    18121 gtttcgtctt cgtgatccag tttgtacttg ttgacaccaa cgatgacacg cttgccctgg
    18181 tcgatcagcg actgttcgcg cgctgaggcc tcttcgatca ttcgttttgg cagacctgct
    18241 tcgatcgctt tcgccatgcc accggcttcg tcgatctgtt ggataatagc tctggcttgt
    18301 ttgacgattt gatcggtcag cgactcaatg taataggatc cggccagtgg atcgacggtg
    18361 tggcagagtt ctgattcttc ctggatgatg atctgggtgt tgcgggcaat gcgtgctgag
    18421 aaatcggtag gcaaaccaag cgcttcgtca aaggcgttgg tatgcagtga ctgagtaccg
    18481 cccagcgtcg cagccagtgc ttcaatggtg gtgcggataa cgttgttata cggatcctgt
    18541 tcagtcaggc tccagcctga ggtctggcag tgggtacgca gcgccagtga tttcggatcc
    18601 tgtgcgccaa atccactgac cgcttcgctc cataaataac gtgccgcacg caacatggcg
    18661 acgttcataa acagatccat gccgataccg aagaataacg acaggcgagg agcgaagtca
    18721 tcaattttca gtccggcaga gattgctgct ttgatgtact caatcccatc ggcgagcgta
    18781 aatgctacct gctgcacgca gttggcaccc gcttccccca tgtggtaacc gctgatactg
    18841 atggtattaa atcgcggcat gttgccggaa caccaggcga tgatgtcggc gataatgcgc
    18901 attgacggtt ttggtgggta aatataggtg ttgcggcaga ggtactcttt gagaatatcg
    18961 ttttgaatgg tgccggtcag tttatcaggt gtaacacctt gctcttctgc ggcgacgata
    19021 taaaacgcca gtactggtag cactgcgcca ttcatggtca tcgaaaccga cattttatcc
    19081 agcgggatct ggtcgaacag gactttcata tcttccacgg tgtcgatagc gacgcccgct
    19141 ttgccgacgt cacccgccac gcgtgggtta tcggagtcgt agccacggtg ggtggcaagg
    19201 tcaaacgcaa cggaaagacc tttttgcccg gcggccaggt tacggcgata aaaagcgttg
    19261 gactcttttg ctgttgaaaa accagcatac tgacggatgg tccacggttg ggcggtatac
    19321 atagtggcac gcgggccacg aacgtagggc ggcaaaccag gaagggtacc tgtcacctcc
    19381 agattatcga gatcggcttc ggtatacagc ggcttgatgg cgatcccttc cgcggtttgc
    19441 tgaaccagcg agtcgacagt tttctcccga cggctcaatt ccttgttggc aagctgttgc
    19501 cactcctgct cgttagacat tatttgctcc gtaaaacgca atcaaaatga gggtatttgc
    19561 gcttacggtg agctttttac gtgggtttgt cgccagcaaa aaaaatgcat tcagacgtcg
    19621 ttttttgccg cctgagattt gtctattttg tgatgtgatt caaggaatac aaaaagaagt
    19681 gaggacaacg cctgatatgt gctatcaggc attatttgat ggattaatcc tgacgaagga
    19741 ctttgtgacc ataatcgagt aacgcatcag tgactttacg catcatgcgg ctttcaggag
    19801 caaagcggtg ccagtagagc atccgtcgtt gaaatagccc aggcgttaag tcaatcagtt
    19861 caccgctggc cagctctttc tcgatttgca ggtgcgggat catacagcag gtggtgccct
    19921 ggcgagcaag ttgtacgaac gcttctgaag aattaacgat atggcagggt acgctgccag
    19981 gaggaagatc gaagttttgc tgcaaaaagg cctggtgcat atcgtcaaga tggtcaaacg
    20041 cgaccactgg cgctttcaat aatgccgaac gcgttacgcc gttaggaaaa tatttttcgg
    20101 caaacggttt tgagctgacg aacagatagt cgagcgcacc gagtttatcg acaagacaac
    20161 tcggcagcgc ctgatgttga atactcaccg cgccgaccac ttcgccgcgg cgcagacgtt
    20221 cctgagtacg ggtttcatct tctacctgca agttgaggcg gataggcgaa tcagccaaca
    20281 caggagccag tgccggaagc aaccacgtcg ccagactgtc ggcgttgacc gccagtgaaa
    20341 gcagcagcgg agtcgaaccg gtttgttcat cgcccagcca ctcttcttcc agcaactcca
    20401 cctggcgcag cagtgccagc agtttttgcc cttgttccgt cgggcgcggc ggtacggtac
    20461 gcaccaacag cggctgcccg aacatatttt ccagttgctt aatgcgctgt gagacggctg
    20521 attgtgtaat gcacagcttt tgtgccgcgc gctcaaatcc tcgttcacgt atcaccgcat
    20581 ccagtgcctg taatgttctg tagtccgggc gtttcattgc tgttgtctgc tcctggtaaa
    20641 tgctttttcc tgcactatga cacaattttg tgtcaggttg catataccga agccgtcggc
    20701 gggtaacatt tgtatcaatg gttaaacgcg cagcttaaaa gatcagggat ttgcgctatt
    20761 gcatttcgct tagcgacttc gcgccctggt tatgttgaat ggcgtggcgt tattgcctct
    20821 gttcgcaccg tggagcggca tacccagacc gttgtatgct ttatttgcgg ggcgaaaggg
    20881 gatgcccgcc attgcgcgat attttatgga tgagttaacc acccggcttg ccaacggggt
    20941 ctgaatcgct ttttttgtat ataatgcgtg tgaaatttca taccacaggc gaaacgatca
    21001 tgacgcagga tgaattgaaa aaagcagtag gatgggcggc acttcagtat gttcagcccg
    21061 gtaccattgt tggtgtaggt acaggttcca ccgccgcaca ctttattgac gcgctcggca
    21121 cgatgaaagg ccagattgaa ggggccgttt ccagttcaga tgcttccact gaaaaactga
    21181 aaagcctcgg cattcacgtt tttgatctca acgaagtcga cagccttggc atctacgttg
    21241 atggcgcaga tgaaatcaac ggccacatgc aaatgatcaa aggcggcggc gcggcgctga
    21301 cccgtgaaaa aatcattgct tcggttgcgg aaaaatttat ctgtattgcc gacgcctcca
    21361 agcaggttga tattctgggt aaattcccgc tgccagtaga agttatcccg atggcacgta
    21421 gtgcagtggc gcgccagctg gtgaaactgg gcggtcgtcc ggaataccgt cagggcgtgg
    21481 tgaccgataa tggcaacgtg atcctcgacg tccacggcat ggaaatcctt gacccgatag
    21541 cgatggaaaa cgccataaat gcgattcctg gcgtggtgac tgttggcttg tttgctaacc
    21601 gtggcgcgga cgttgcgctg attggcacac ctgacggtgt caaaaccatt gtgaaatgat
    21661 ctgacggggg aacctccccc gttaaaaaaa ttctcttcat taaatttggt gacatgtgtc
    21721 acgcttttac caagcaattg tcgattgctc taaataaatc ctctaaacca gcatattcat
    21781 ccaagaatta cctttgcgtg atatttcctc aacatcgcga cgcaaacgtt catattgccg
    21841 caatattatt ttttgatatg ttgaaaggcg gatgcaaatc cgcacacaac atttcaaaag
    21901 acaggattgg gtaaatggca aaggtatcgc tggagaaaga caagattaag tttctgctgg
    21961 tagaaggcgt gcaccaaaag gcgctggaaa gccttcgtgc agctggttac accaacatcg
    22021 aatttcacaa aggcgcgctg gatgatgaac aattaaaaga atccatccgc gatgcccact
    22081 tcatcggcct gcgatcccgt acccatctga ctgaagacgt gatcaacgcc gcagaaaaac
    22141 tggtcgctat tggctgtttc tgtatcggaa caaaccaggt tgatctggat gcggcggcaa
    22201 agcgcgggat cccggtattt aacgcaccgt tctcaaatac gcgctctgtt gcggagctgg
    22261 tgattggcga actgctgctg ctattgcgcg gcgtgccgga agccaatgct aaagcgcacc
    22321 gtggcgtgtg gaacaaactg gcggcgggtt cttttgaagc gcgcggcaaa aagctgggta
    22381 tcatcggcta cggtcatatt ggtacgcaat tgggcattct ggctgaatcg ctgggaatgt
    22441 atgtttactt ttatgatatt gaaaacaaac tgccgctggg caacgccact caggtacagc
    22501 atctttctga cctgctgaat atgagcgatg tggtgagtct gcatgtacca gagaatccgt
    22561 ccaccaaaaa tatgatgggc gcgaaagaga tttcgctaat gaagcccggc tcgctgctga
    22621 ttaatgcctc gcgcggtact gtggtggata ttccggcgct gtgtgatgcg ctggcgagca
    22681 aacatctggc gggggcggca atcgacgtat tcccgacgga accggcgacc aatagcgatc
    22741 catttacctc tccactgtgt gaattcgaca acgtccttct gacgccacac attggcggtt
    22801 cgactcagga agcgcaggag aatatcggct tggaagttgc gggtaaattg atcaagtatt
    22861 ctgacaatgg ctcaacgctc tctgcggtga acttcccgga agtctcgctg ccactgcatg
    22921 gtggtcgtcg tctgatgcac atccacgaaa accgtccggg cgtgctaact gcgctgaaca
    22981 aaatcttcgc cgagcagggc gtcaacatcg ccgcgcaata tctacaaact tccgcccaga
    23041 tgggttatgt agttattgat attgaagctg acgaagacgt tgccgaaaaa gcgctgcagg
    23101 caatgaaagc tattccgggt accattcgcg cccgtctgct gtactaattc cccttctctg
    23161 aaaattaacg ggcaggtcac tgacttgccc gtttttttat cccttctcca caccgttgcc
    23221 gcgttgcgca ttctactttg cgagtctgct ccagaaaata aatcaaagtc agatcaatgc
    23281 gttgtgttta tttaatgggg atttacgatg gcgggacagc atggggctgt aacggtaaag
    23341 ccctcaccga agcgagggct taaaggagaa gggttatgat gcgacttgtc atcatactga
    23401 ttgtactgtt actgataagt ttcggagctt attaacagtc agtctcaagg gaggagtcat
    23461 cctcccttac ccttactcac taaattaggt caaagaatca acgatgtcaa tcagggcgat
    23521 gcggggtgca tcgccctgaa ccgaactttt tgatagccac ttcatcgccc acgtttactg
    23581 ccgtaataga tcctttttcc caaaaattat ctctgttttg catggggttt ttcgagcacg
    23641 ctcgaagaaa agatttaaag gtagatcaat gcgttgtggt tatttaaaga gagcttacga
    23701 tagctgggca gcatggggct gtaacggtaa agccctcacc gaagcgaggg cttgaaggag
    23761 aagggttatg atgcgacttg ccatcatact gattgtactg ttactcataa gtttcccagc
    23821 ttattaacag tcagtcacag gggaggagca atcctccctt acccttactc actaaattag
    23881 gttaaagaat caacgatgtc aatcagggcg atgcaggtgt atcgccctta ccactcccag
    23941 actttcgacg gtgtaaccac cgcaggaaga gggatatccc actcttcaac ggggagtttt
    24001 tccaccaact gacaatcatg cgcataaccc accggttgcg ttttatagtg ctgccagttt
    24061 tgtaaggtcc gatcataaaa accaccgccc attcccagac gctgaccgta ctcatcaaag
    24121 gcgaccagcg gtgtgatcag cacgtctaat cgggaaaggg gtagcacgtc acgcacatcc
    24181 agttttggct catggatctt caacctgttc atcaccagtt cgctttgcgg atggtaattc
    24241 aggaacagca aattaccggc actaaaggga tgcaaaactg gaagatatac gcgcttaccg
    24301 gcgcgccaga gttgttctat tagcggctgg gtgtcgagtt caccatcaaa agagaggaat
    24361 acagcgaccg tatgcgccat caccaccggg ggataagtca tcatccgggt agcggcttgt
    24421 tgacccattt ccagctgttg ttccggcgtt aacgcacgac gacgttgccg aatcattttg
    24481 cggatttctt gtcgggataa tgtcagtggg agctctggta gttgcgtcat ggctggtgcc
    24541 agataagaag ggaatctccg agatgccgcc gcaggctgta acccttgaac ccttggttca
    24601 aggtgaatgt gtcgtcgcag ttttaaggct tctcggacga accgagcatg ctcaccaacc
    24661 gcggagcgcc acattcttgt ggtatgaaat atcggctcag gggactggcc cgcttgcgaa
    24721 catctcagag aaattttgtc ttcacggtta ctctaccaca gtaaaccgaa aagtgtcatt
    24781 caaagttttg gttagttttt tcggtgatgc gaccttgttc aagtaacgct tgttctatgg
    24841 tctgctgcag catccgaata cgctgttcca tacttgccgc gtagtcacga gtctttgctt
    24901 tttcttgcgc taactcatag ctgatattca atgcggcaat gaagaccaac tgttcagtat
    24961 ttgtgactct agtgcgttct ttcagatctt gcaaccgttg gttcagatcg tccgctgcct
    25021 gattcaacgc atccctttgg tcaggcgggc agttcacacg cagtgaacgg ccaaaaattt
    25081 ggatatcgac gggttgtgca gacatgccac cttcctgctg attgactgcg ctgccttcgt
    25141 ctttagaccc atggtctgcg aaggggcgac actatagcta ccctgatgag aagagacaag
    25201 cccttttctg gtccaccagg gcccaaagtg gtagcatatc atgaatattc ctccctttga
    25261 cgacgaatgc ttatgtctat acagaacgaa atgcctggtt acaacgaaat gaaccagtat
    25321 ctgaaccaac aagggacggg tctgacccct gccgagatgc atggtttaat cagtgggatg
    25381 atatgtggcg gtaacgatga cagctcatgg ctgccgctac ttcacgacct gacgaacgaa
    25441 ggcatggctt tcggtcatga gctggcacag gcactgcgta aaatgcactc tgccaccagc
    25501 gatgccctgc aggatgacgg cttccttttt cagctttatc tgcctgatgg cgatgatgtc
    25561 agcgttttcg atcgggctga tgcactggca ggttgggtca atcacttcct gcttggtctt
    25621 ggcgttacgc aaccgaagct ggacaaagtg accggcgaaa ccggtgaagc catcgacgat
    25681 ctgcgtaaca tcgcgcagct gggctacgac gaagacgaag atcaggaaga gcttgaaatg
    25741 tcgcttgaag agatcatcga atacgttcgt gttgccgcgc tgttatgcca cgacaccttt
    25801 actcatccgc aaccgaccgc gccagaagta caaaaaccga ctctacacta aaaacaaaaa
    25861 acgtaaggag agtgttatga gtgagatatc ccggcaagag tttcagcgtc gccgtcaggc
    25921 cctggtggag caaatgcaac ccggcagcgc cgcgctgatt tttgctgcac cagaagtaac
    25981 acgtagcgcc gacagcgaat acccctatcg tcagaacagt gacttctggt acttcaccgg
    26041 ctttaacgaa ccggaagcgg tgctggtgct gattaaaagc gatgacactc ataaccacag
    26101 cgttctgttt aaccgcgttc gcgacctgac ggcggagatc tggtttggcc gtcgcttagg
    26161 ccaggatgcc gcgccagaga aactgggcgt tgaccgcgca ctggcattca gcgaaatcaa
    26221 tcagcaactt tatcaactac ttaacggcct ggatgtggtt taccatgccc agggcgaata
    26281 tgcatatgct gatgaaatcg tgaacagtgc gctggaaaaa ctgcgtaaag gttcgcggca
    26341 aaatctcacc gcaccggcaa cgatgatcga ctggcgtcct gttgttcatg aaatgcgcct
    26401 gttcaaatcg ccagaagaga ttgccgtact ccgccgcgcg ggagaaatca ccgccatggc
    26461 acatacacgg gcgatggaaa aatgccgtcc gggaatgttc gagtaccatc tggaaggcga
    26521 aattcaccac gaatttaacc gccacggtgc gcgctatcct tcctataaca ccattgtagg
    26581 cagcggtgaa aacggctgca ttctgcacta caccgaaaac gagagtgaac tgcgcgacgg
    26641 tgacctggtg ttgattgacg cgggttgtga atacaaaggt tacgcgggcg atattacccg
    26701 caccttcccg gtcaacggca aattcaccca ggcccagcgt gaaatctacg acattgtgct
    26761 ggagtctctc gaaaccagcc tgcgcctgta tcgtccggga acttccattc tggaagtcac
    26821 tggtgaagtg gtgcgcatca tggttagcgg cctggtaaaa ctcggcatcc tgaaaggtaa
    26881 tgttgatgaa ctgatcgctc agaacgccca tcgtcctttc tttatgcatg gccttagcca
    26941 ctggttagga ctggatgtcc atgacgttgg cgtttatggt caggatcgct cgcgcattct
    27001 ggaaccgggg atggtactga ccgtagagcc tggtctgtat atcgcgccgg atgcggatgt
    27061 gccagaacaa tatcgcggta tcggcattcg tattgaagac gacattgtga ttaccgaaac
    27121 cggcaacgaa aacctcaccg ccagcgtggt gaaaaagccg gaagaaatcg aagcgttgat
    27181 ggctgctgcg agaaagcaat gagcgtaatc atcgtcggtg gcggcatggc gggcgcgacg
    27241 ctggcgctgg ctatttcccg gttaagtcac ggggcgctgc cggtacattt gattgaagcg
    27301 actgcgccag agtcacatgc tcatccgggc tttgatggac gagcgatagc gctggcggcg
    27361 ggtacctgtc agcaactggc gcgcatcggc gtctggcaat ctctggctga ttgcgcaacc
    27421 gccatcacta ccgtgcatgt cagcgatcgt ggtcatgctg gattcgtcac cctcgccgca
    27481 gaagattacc aactggcggc gctggggcag gttgtcgaat tgcataatgt cgggcaacgg
    27541 ctgtttgcat tgctgcgaaa agcacctggc gtaatgctgc attgccccga tcgcgtagct
    27601 aacgttgccc gtactcagag tcatgttgaa gtgacgctgg agagcggcga gatgctgacg
    27661 ggccgcgtgc tgatagccgc tgatggcacc cattcagcgt tagccaccgc gtgcggcgtt
    27721 gattggcagc aggagcctta cgaacaactg gcagtgattg ccaacgttgc tacttctgtt
    27781 gcgcacgaag gccgcgcttt tgaacgcttc acgcaacatg gcccgctggc gatgttgccg
    27841 atgtctgacg gacgctgttc gctggtctgg tgtcatccac tggaacggcg cgaagaggtg
    27901 ctgtcgtgga gcgacgagaa gttttgccgt gaactccagt ctgcctttgg ctggcgactg
    27961 gggcaaatta cccacgccgg taaacgcagt gcttatccgc tggcattaac ccgcgccgcc
    28021 agaccgatta cccatcgcac tgtgctggtg ggcaatgcgg cgcaaactct gcacccgatt
    28081 gccgggcaag ggtttaacct cggtatgcga gatgtcatga gccttgcgga aaccctgact
    28141 caggcgcatg agcgcggaga agacatcggt gattacggca tattgtgccg ctatcagcag
    28201 cgtcgacaga gcgatcgcga agcaaccatc ggcgtcacgg acagcctggt acatcttttt
    28261 gccaaccgtt gggcaccgct ggttgtcggg cgcaacatcg gactgatgac gatggaatta
    28321 ttcaccccgg cacgcgatgt gctggcgcag cgcaccctcg gttgggtggc gcgttgaagg
    28381 cggttttaag gaataaacaa tgcaaagtgt tgatgtagcc attgttggcg gcggcatggt
    28441 ggggctggcg gttgcctgcg gcttacaggg gagcggctta cgcgttgccg tactggagca
    28501 gcgcgtaccg gaacctctgg cggtagatgc actaccacaa ctgcgcgttt cggctatcaa
    28561 tgccgccagc gaaaaattac tcacccgtct tggcgtctgg caggacattc tcgctcgtag
    28621 ggccagttgt tatcacggca tggaagtgtg ggacaaagac agttttggtc acatctcgtt
    28681 tgacgatcaa agcatgggct atagccatct tgggcacatc gttgaaaact cagtgattca
    28741 ctacgcgctg tggaacaaag cgcagcagtc gtcagatatt actctgttgg cccccacaga
    28801 attacagcag gtcgcctggg gagaaaatga aaccttcctg acgctgaaag atggcagcat
    28861 gttaacggcg cgtctggtga ttggcgcgga cggtgctaat tcctggttgc gcaacaaagc
    28921 agatattccg ctgactttct gggattatca gcatcacgcg ctggtagcga ccattcgcac
    28981 ggaagaaccg catgatgcgg tggcgcggca ggttttccat ggcgaaggca ttctggcctt
    29041 tttaccgctt agcgatccgc atctttgctc gattgtctgg tcactgtcgc cagaggaagc
    29101 gcagcggatg cagcaggcaa gtgaagacga atttaatcgc gcgttaaata tcgcttttga
    29161 taatcgcctt ggcttatgca aggttgagag cgcgcgtcag gtgttcccgc tgacggggcg
    29221 ttatgcgcgc cagtttgccg cacaccgtct ggcgctggtg ggcgacgccg cgcataccat
    29281 tcacccactg gcggggcagg gcgttaatct cggatttatg gacgccgcag agctgattgc
    29341 tgaactgaaa cggttgcatc gtcagggtaa agacatcggg cagtacattt acctgcgccg
    29401 ctatgaacgt agccgcaagc acagcgcggc gctgatgctg gctggtatgc agggttttcg
    29461 cgatctcttt tccggcgcta atccggcgaa aaaactgctg cgtgatattg gtttgaaact
    29521 ggccgacacg cttcctggcg ttaagccgca acttatccgc caggcaatgg gattaaacga
    29581 tttgcctgaa tggctgcgtt aaaaatttct cctctgttgt ttatttgata cccatcacac
    29641 tttcatctcc cggttttttc gccgggagat tttcctcatt tgaaataaac taatttcacc
    29701 tccgttttcg cattatattt tctaatacca tgattttttg atttagtgtt ttttgacatt
    29761 tttttagctc ttaatattgt cttattcaaa ttgactttct catcacatca tctttgtata
    29821 gaaattggtg tattttttgg ttttttattc tgtcgcgatt tttgcatttt ttaaccataa
    29881 gctaatgtga tgatcaattt taccttatgg ttaacagtct gtttcggtgg taagttcagg
    29941 caaaagagaa cgattgcgtt ggggaccggg agtggctccg atgctgggtt ttgtggtgat
    30001 aatttcacca tgaaaaagtt gtcagccccg cttattcaat gaggacaaga tggcacaaca
    30061 gactcctttg tacgaacaac acacgctttg cggcgctcgc atggtggatt tccacggctg
    30121 gatgatgccg ctgcattacg gttcgcaaat cgacgaacat catgcggtac gtaccgatgc
    30181 cggaatgttt gatgtgtcgc atatgaccat cgtcgatctc cgcggcagcc gcacccggga
    30241 gtttctgcgt tatctgctgg cgaacgatgt ggcgaagctc accaaaagcg gcaaagccct
    30301 ttactcgggg atgttgaatg cctctggcgg tgtgatagat gatctcatcg tctactactt
    30361 tactgaagat ttcttccgcc tcgttgttaa ctccgccacc cgcgaaaaag acctctcctg
    30421 gattacccaa cacgctgaac ctttcggcat cgaaattacc gttcgtgatg acctttccat
    30481 gattgccgtg caagggccga atgctcaggc aaaagctgcc acactgttta atgatgctca
    30541 gcgtcaggcg gtggaaggga tgaaaccgtt ctttggcgtg caggtgggcg atctgtttat
    30601 tgccaccact ggttataccg gtgaagcggg ctatgaaatt gcgctgccca atgaaaaagc
    30661 ggccgatttc tggcgtgcgc tggtggaagc gggcgttaag ccctgcggtc tgggggcgcg
    30721 tgacacgctg cgtctggaag cgggcatgaa tctttatggt caggagatgg acgaaactat
    30781 ttctccttta gccgccaaca tgggctggac tatcgcctgg gaaccgacag atcgtgactt
    30841 tatcggtcgt gaagccctgg aagtgcagcg tgagcatggt acagaaaaac tggttggtct
    30901 ggtgatgacc gaaaaaggcg tgctgcgtaa tgaactgccg gtacgcttta ccgatgcgca
    30961 gggcaaccag catgaaggca ttatcaccag cggtactttc tccccgacgc tgggttacag
    31021 cattgcgctg gcgcgcgtgc cggaaggtat tggcgaaacg gcgattgtgc aaattcgcaa
    31081 ccgtgaaatg ccggttaaag tgacaaaacc tgtttttgtg cgtaacggca aagccgtcgc
    31141 gtgatttact tttttggaga ttgattgatg agcaacgtac cagcagaact gaaatacagc
    31201 aaagaacacg aatggctgcg taaagaagcc gacggcactt acaccgttgg tattaccgaa
    31261 catgctcagg agctgttagg cgatatggtg tttgttgacc tgccggaagt gggcgcaacg
    31321 gttagcgcgg gcgatgactg cgcggttgcc gaatcggtaa aagcggcgtc agacatttat
    31381 gcgccagtaa gcggtgaaat cgtggcggta aacgacgcac tgagcgattc cccggaactg
    31441 gtgaacagcg aaccgtatgc aggcggctgg atctttaaaa tcaaagccag cgatgaaagc
    31501 gaactggaat cactgctgga tgcgaccgca tacgaagcat tgttagaaga tgagtaacgg
    31561 ctttattcct cttctgcggg agaggatcag ggtgaggaaa atttatgcct caccctcact
    31621 ctcttcgtaa ggagagaggt tcacaattca ctgcacgttt caggaaccat cgctcatgac
    31681 acagacgtta agccagcttg aaaacagcgg cgcttttatt gaacgccata tcggaccgga
    31741 cgccgcgcaa cagcaagaaa tgctgaatgc cgttggtgca caatcgttaa acgcgctgac
    31801 cggccagatt gtgccgaaag atattcagct tgcgacacca ccgcaggttg gcgcaccggc
    31861 gaccgaatac gccgcactgg cagaactcaa ggctattgcc agtcgcaata aacgcttcac
    31921 gtcttacatc ggcatgggtt acaccgccgt gcagctaccg ccggttatcc tgcgtaacat
    31981 gctggaaaat ccgggctggt ataccgcgta cactccgtat cagcctgaag tctcccaggg
    32041 ccgccttgaa gcactgctca acttccagca ggtaacgctg gatttgactg gactggatat
    32101 ggcctctgct tctcttctgg acgaggccac cgctgccgcc gaagcaatgg cgatggcgaa
    32161 acgcgtcagc aaactgaaaa atgccaaccg cttcttcgtg gcttccaatg tgcatccgca
    32221 aacgctggat gtggtccgta ctcgtgctga aacctttggt tttgaagtga ttgtcgatga
    32281 cgcgcaaaaa gtgctcgacc atcaggacgt cttcggcgtg ctgttacagc aggtaggcac
    32341 taccggtgaa attcacgact acactgcgct tattagcgaa ctgaaatcac gcaaaattgt
    32401 ggtcagcgtt gccgccgata ttatggcgct ggtgctgtta actgcgccgg gtaaacaggg
    32461 cgcggatatt gtttttggtt cggcgcaacg cttcggcgtg ccgatgggct acggtggccc
    32521 acacgcggca ttctttgcgg cgaaagatga atacaaacgc tcaatgccgg gccgtattat
    32581 cggtgtatcg aaagatgcag ctggcaatac cgcgctgcgc atggcgatgc agactcgcga
    32641 gcaacatatt cgtcgtgaga aagcgaactc caacatttgt acttcccagg tactgctggc
    32701 aaacatcgcc agcctgtatg ccgtttatca cggcccggtt ggcctgaaac gtatcgctaa
    32761 ccgcattcac cgtctgaccg atattctggc ggcgggcctg caacaaaaag gtctgaaact
    32821 gcgccatgcg cactatttcg acaccttgtg tgtggaagtg gccgacaaag cgggcgtact
    32881 gacgcgtgcc gaagcggctg aaatcaacct gcgtagcgat atcctgaacg ccgtggggat
    32941 cacccttgat gaaaccacca cgcgtgaaaa cgtaatgcag cttttcagtg tgctgctggg
    33001 cgataaccac gggctggaga tcgacacgct ggacaaagac gtggctcacg acagccgctc
    33061 tatccagcct gcgatgctgc gcgacgacga aatcctcacc catccggtgt ttaatcgcta
    33121 tcacagcgaa accgaaatga tgcgctatat gcactcgctg gagcgtaaag atctggcgct
    33181 gaatcaggcg atgatcccgc tgggttcctg caccatgaaa ctgaacgccg ccgccgagat
    33241 gatcccaatc acttggacgg aatttgccga actgcacccg ttttgcccgc cggaacaggc
    33301 tgaaggttat cagcagatga ttgcgcagct ggctgactgg ctggtgaaac tgaccggtta
    33361 cgacgccgtt tgtatgcagc cgaactccgg cgcacagggc gaatacgcgg gcctgctggc
    33421 gattcgtcat tatcatgaaa gccgcaacga agggcatcgc gatatctgcc tgatcccggc
    33481 ttctgcgcac ggaactaacc ccgcttctgc acatatggca ggaatgcagg tggtggttgt
    33541 ggcgtgtgat aaaaacggca acatcgatct gactgatctg cgcgcgaaag cggaacaggc
    33601 gggcgataac ctctcctgta tcatggtgac ttatccttct acccacggcg tgtatgaaga
    33661 aacgatccgt gaagtgtgtg aagtcgtgca tcagttcggc ggtcaggttt accttgatgg
    33721 cgcgaacatg aacgcccagg ttggcatcac ctcgccgggc tttattggcg cggacgtttc
    33781 gcacctcaac ctgcataaaa ctttctgcat tccgcacggc ggtggtggtc cgggtatggg
    33841 accgatcggt gtgaaagcgc atctggcacc gtttgtaccg ggtcatagcg tggtgcaaat
    33901 cgaaggcatg ttaacccgtc agggcgcagt ttctgcggca ccgttcggta gcgcctctat
    33961 cctgccaatc agctggatgt acatccgcat gatgggcgca gaagggctga aaaaagcaag
    34021 ccaggtggca atcctcaacg ccaactatat tgccagccgc ctgcaggatg ccttcccggt
    34081 gctgtatacc ggtcgcgacg gtcgcgtggc gcacgaatgt attctcgata ttcgcccgct
    34141 gaaagaagaa accggcatca gcgagctgga cattgccaag cgcctgatcg actacggttt
    34201 ccacgcgccg acgatgtcgt tcccggtggc gggtacgctg atggttgaac cgactgaatc
    34261 tgaaagcaaa gtggaactgg atcgctttat cgacgcgatg ctggctatcc gcgcagaaat
    34321 tgaccaggtg aaagccggtg tctggccgct ggaagataac ccgctggtga acgcgccgca
    34381 cattcagagc gaactggtcg ccgagtgggc gcatccgtac agccgtgaag ttgcggtatt
    34441 cccggcaggc gtggcagaca aatactggcc gacggtgaaa cgtctggatg atgtttacgg
    34501 cgaccgtaac ctgttctgct cctgcgtacc gattagcgaa taccagtaat tcactgattc
    34561 gagtattttc taaaggcgct tcggcgcctt tttagtcaga tgacaaagta caaaagtgct
    34621 cagacagtcc cctcgcccct ttggggagag ggttagggtg aggggaacag gccggcactg
    34681 gcgcgaatat ttaccctcac cccgaccctc tccctgaaag ggcgaggggg aaaagcgtgc
    34741 caacattgaa gattgagcca gtttgttagc aatctcaaag atacgtcaac gaattaattt
    34801 ttctcggaaa aacaaatggc tatagcactt gtgaccggtg gcagtcgcgg catcgggcgg
    34861 gcaactgcat tactgttggc gcaagaaggg tatacggtgg cggttaatta tcagcaaaac
    34921 ctccacgcgg cgcaggaagt gatgaactta ataatgcaag ccggtggcaa agcattcgtg
    34981 ctccaggcgg atatcagcga cgaaaaccag gtcgttgcga tgtttacagc aatcgatcag
    35041 cacgatgaac cgctagcagc gctggtcaat aacgccggga tcttgtttac ccagtgcacc
    35101 gttgaaaacc ttaccgcaga gcgaatcaac cgagtacttt ccaccaacgt gacgggatat
    35161 tttctctgct gccgcgaggc ggtaaaacgc atggcgctta aaaatggtgg cagtggcggc
    35221 gctatcgtca atgtctcttc ggtggcctca cggttgggtt cgccagggga atatgttgat
    35281 tacgcggcat cgaaaggggc gattgattcg ttaaccaccg gactatcgct ggaagtcgcc
    35341 gcgcagggga tccgcgttaa ctgcgtgcgg ccagggttta tttataccga aatgcacgcc
    35401 agcggcggcg agcctggacg cgtcgatcgc gttaagtcga acatccccat gcagcgtggt
    35461 ggacaggcag aagaggtcgc gcaggccatt gtctggctac taagtgataa agcctcttac
    35521 gtcacgggaa gttttatcga tttggcgggc gggaaataaa acagggaagt tgtctgaccg
    35581 gatgcaacaa gtattgcatc cggtacttca tcgacttaaa gcttctcgcc gttgctggca
    35641 atcacctctt tgtaccagtt aaagctcttc ttacgtgaac gcgacatatc gccagtaccg
    35701 tcgtcatgtt tattcacata gataaagccg tagcgtttgc tgtactgccc ggtagtgaat
    35761 gacacgcagt cgatgcagcc ccacggtgtg tagcccatca gatccacacc atcgtaagtc
    35821 actgcttttt tcatctcttc aatatgggcg cgcaggtagt caatgcggta gtcgtcgttg
    35881 atgctgccat cttcttccac tttgtcgtaa gcgccaaaac cgttttcgac aataaacagc
    35941 ggcctctgat aacgctcata cagttcgcaa agggcatagc gcagacctac tggatcaatc
    36001 tgccagcccc agtcagacgc tttaacatac gggttcggta cgctgccttc aaaaccagag
    36061 atcgcatcgc cggtgccgcc ttcggccttc actgcgttgg tcatgtaata gctgaaacca
    36121 agataatcgc aggtgccttc gcgcagtaca tcgagatcgc cgtcttccat tttgatatta
    36181 aatccgcgac gttcccactc gttcaataca taggacgggt aatagccgca tagctgcaca
    36241 tcggtaaaga cgtagcgttc gcgcatcgac tcctgagcga acatcacatc gtccgggtta
    36301 catgagtagg gatagagcgg caccatcgcc agcatacagc cgactttcat ctccgggtta
    36361 atgcgacgcg cggctttcac tgccagggcg ctggcgacaa actggtgatg cagcacctgg
    36421 tacatcgtct cttccgggtt gtcatgctcg gtatacacca cgccggagca gcagtaacca
    36481 aacagcggcg cacgccagtt acgctggttg tttatttcgt tgaaggtcat ccagtatttg
    36541 actttatgct tgtagcgttc gaataccact tccgcgaaac ggacaaagaa atcaacgaat
    36601 ttacggttgg tccagctacc gtattgctgc accagatgca gcggcatttc aaagtgggag
    36661 agggttatca ccggttcgat gttgtatttc agcagttcat cgaacagaga gtcgtaaaac
    36721 ttcagccctt cttcgtttgg ctgagcttca tcaccttttg gaaaaatacg cgtccaggcg
    36781 atggaagtcc gaaaacattt gaagcccatt tcggcaaata gcttgatgtc ttccttgtag
    36841 tgaccataaa aatcaacggc ttcatggttt ggatagtatt ttcctggcaa gacttctttg
    36901 gtgatttcgc gcggcacgcc gtgtgcgcca caggtcagaa cgtcacaaat gctcggtcct
    36961 ttgccgcctt tgttccagcc gccttcgacc tgatgagcgg caacggcgcc accccataag
    37021 aaatctttcg gtaaggtgag ttttttcact atcataggct ccagatcagt caattcttac
    37081 aggaaatagt accatgcagc caaacgacat cacttttttc aacgtttcca ggatgacatt
    37141 ctggctgggc gtaaaaccat caccatccgc gacgagtctg aatcgcactt caaaacgggt
    37201 gatgtgcttc gtgtcggacg ttttgaagat gacggttatt tttgcacgat tgaagtcacc
    37261 gcaacctcaa ccgtaacgct ggatacactg acggaaaaac atgcagagca ggaaaatatg
    37321 accctgcctg aactgaaaaa ggtcattgct gacatctatc ccggtcagac acaattttat
    37381 atgattgaat ttaaatgtct ttaaattgaa ctcacgcctg ttagttgaaa tttcgcattt
    37441 aatttcatga tttttaggag attagtctca aatcagaatt tattttagct aacaggtgtt
    37501 cactggaact attctcagtt acgctggaga ggtatacatg tgcgtgtacg tttctctgga
    37561 gtaagttatg gttcagaagc ccctcattaa gcagggatat tcgctggcag aggaaattgc
    37621 caacagcgtc agtcacggca ttgggctggt gtttggtatc gttgggctgg tgttgctact
    37681 ggttcaggcg gtggatctca atgccagcgc cacagcgata accagttaca gcctctatgg
    37741 cggcagtatg atcctgctgt tcctcgcttc gacgctctat cacgccattc ctcatcaacg
    37801 ggcaaaaatg tggctgaaga aatttgacca ttgcgctatt tacctgttga ttgccggaac
    37861 ctacacgccg tttttgctgg tggggctgga ttctccgtta gcgcgcgggt tgatgattgt
    37921 tatctggagc ctagcattgc tggggattct gttcaaactg accatcgcgc accgattcaa
    37981 aattttatct ctggtgactt atctggcgat gggctggctg tcgctggtgg taatttatga
    38041 aatggcagtt aagctcgcgg cgggcagcgt taccttactg gcggtaggcg gcgtggttta
    38101 ttcgctcggg gtgattttct acgtctgcaa acgcattcca tacaaccatg ccatctggca
    38161 cggcttcgtg ctcggcggta gtgtgtgcca ctttctggcg atctatttgt atattgggca
    38221 ggcgtaatgg gtatgccttt gtaggcctga taagacgcgt cagcgtcgca tcaggcatcg
    38281 gagcgcaacc gccggatgcg gcgtgaacgc cttatccgat ctacgggcct cgtccctgtt
    38341 gtgataaagg catcaaatcc cagacatgat aagacgcgtc agcgtcgcat caggcgataa
    38401 atgcctgatg cgatacagag atttactctt cgagcgaata cggcagcggc tcgatatgca
    38461 gtgtattcgc atcgtcgcgt acgcggaaga tgctgtccgg ttccatatcg ttattcatga
    38521 cgacctgtac cacgacctga ccatcttcca gttttaccgc agccagcacc gtaccggtac
    38581 gacgccagtt ctcgcccatt ttcagctcta agtcttcacc agcttccggc agtcggctgg
    38641 cactacctgc cagcaaccag agcgcgcgtt tgttggcacc acggaatttt gctcgcgcca
    38701 ccatctcttg tccggtataa cagcctttct taaagctgat accgcccagc gcctggagat
    38761 tggtcgcctg tgggataaac tgcccgctgt tggcggcatc aatcaccggg aaaccagctt
    38821 caatgtttaa tgccagccac tgttggctat tgttcagttc cgcttcaccg cgcagtttat
    38881 cggtcagcat attagcagta gcttcatcgg ttacgatcag gaaacgttct gccgggtgtt
    38941 caaaccatag caaagtggtc gcgccttctt tgactacctg tttttctttc gaaggcagtt
    39001 cgctaaagag atttgccagc gcggcgcgcg cctgaaaacc ggcaacacca agcagcacac
    39061 gctcgtcgtc tggcgcgatg gtcactttag agaataccgc atattttttc agttcagtca
    39121 gctgcggttc acgcacgctg cgccgttcaa tccatgcaaa gccatcgccg tcgcggaaca
    39181 gacgtaaatt gctccacatt ttacctttgg cgtcgcaatg ggcggcgagc aggtgctgat
    39241 cttctgccat ctggctgaca tctgctgtca cctgaccctg catatatttt tcgctgtccg
    39301 cgccagtaat ggtggcaagc gcccagtcat caagcgtcat cagcgtcagc ggtaaacggg
    39361 cagaagccgt aggctgacgt ggaggaaaag gtgtaaaagc cataatagag tcctgagttg
    39421 cttaacggcg tattaatgac taatggtaaa agagctatga ggcaatgcaa gtgctttcac
    39481 tacgcgatct gtgccttctg cgaggcgact gccaaaaaaa tgaagccctt gaattgttgt
    39541 gaattaattt cgcgatcgcg atggtgttta ctgatattcc cgtaaaagaa gtcgaaaaac
    39601 acgttacaat agccgggtac tggattttcg tgagacagga agaagatgga cattaacaac
    39661 aaagcccgaa ttcattgggc atgccgccgt ggtatgcgcg aactcgatat ttcaatcatg
    39721 tcgtttttcg aacatgagta cgacagctta agcgatgacg aaaaacgcat ctttattcgt
    39781 ctgctggaat gtgacgatcc ggacctgttt aactggctga tgaatcacgg taaaccggcc
    39841 gatgcagaac tggaaataat ggtccgactc atccagacac ggaaccggga acgtggtcct
    39901 gtggcaatct gatttgcgcg tctcctggcg cgcacagtgg ctttccttgc tgattcatgg
    39961 gctggttgcc gctgttattt tactcatgcc ctggccgctc agttacaccc cgttatggat
    40021 ggtgttactt tcgctggtgg tatttgattg cgttcgcagc cagcggtgta ttaatgctcg
    40081 ccagggggaa attcgcttgt tgatggacgg gcgtttgcgt tggcaagggc aggagtggag
    40141 catcgtcaaa gcaccgtgga tgattaagag cggcatgatg ctgcgtttac gttctgatgg
    40201 cggtaaacgg caacatttat ggctggcagc cgacagcatg gacgaagccg aatggcggga
    40261 tttacggcgg attttgttgc aacaagagac gcaaagataa agatgacggc aaagcatatg
    40321 cgctaagccg tcagtgagat caggcgtaat gctctgccat ttcgttgagg atttgctcgc
    40381 accagctctg aatacgctcg tcgctaaggt catactggtt agtttcatcc agcgccagac
    40441 ccacgaacag ttgcccgtca gcaatcaccg gtttcgggct ggtaaattca tatccttccg
    40501 ttggccagta gccgacgaac ttcacgcctt tggtcgagag tttgtcatgc agcataccga
    40561 gcgcatcgag gaaccactcg ccgtatccca gttgatcgcc aagcccatac agcgcaataa
    40621 ttttaccttc aaggttcagg tcgtcgagct gatcccagac ggcttcccag tcttcctgga
    40681 tttcaccaaa atcccaggtc gggataccca gaatgagcac atcgtactgc tccattaatt
    40741 tcggggagtc gtccttgagg ttatgtaagg tcaccagttc tgggccgata atatcgcgga
    40801 ttttttctgc cgccatttcg gtgtaacagg tgctggaacc gtaaaaaaga cccatattca
    40861 taacgtaagc ttctcgattc tgctgtggct tttgcagcta gtgtatcaga atcgctttag
    40921 tcacggcaca ataaggcata atggatcaaa tgagtgacca taaggggcgc aagtgaaaca
    40981 ggagctggca cgcatcgagc agtttcttga tgctctgtgg ctggaaaaaa atctggctga
    41041 aaatacgttg aacgcttacc gtcgcgatct gtcaatgatg gtggagtggt tgcatcaccg
    41101 cgggttgacg ctggcgacgg cgcaaagtga cgatttgcag gcattactgg cagaacggct
    41161 ggagggcggg tataaagcca ccagctcagc gcgtttgctg agtgcagtgc gccgattgtt
    41221 ccagtatctt tatcgcgaaa agtttcgtga agacgatccc agtgcgcatc tcgcttcacc
    41281 gaaattgccc cagcgtttgc caaaagattt aagcgaagcg caggtcgaac gtttattaca
    41341 ggcgccatta attgatcagc cactggagct acgcgataaa gccatgcttg aagtgttgta
    41401 tgctaccggg ctgcgtgtct ctgaactggt cgggctgaca atgagtgata tcagcctgcg
    41461 tcagggcgta gtgcgggtca ttggtaaagg caacaaagag cgcctggtgc cgttagggga
    41521 agaggcggtt tactggctgg aaacctatct ggaacatggg cgtccgtggc tgctgaatgg
    41581 tgtgtcaatt gacgtgttgt ttcccagcca gcgtgcgcag cagatgacgc gacagacctt
    41641 ctggcaccgc attaaacatt atgccgtgct ggcgggtatc gacagcgaaa agctgtcacc
    41701 gcatgtgttg cgtcacgctt ttgccactca tttattaaat catggtgcgg atttacgcgt
    41761 ggtgcagatg ctactgggcc acagcgatct ttccaccacg caaatttata cgcatgtcgc
    41821 taccgagcgt ctgcggcaac ttcatcaaca gcatcacccg cgggcgtgat gtctgaaaag
    41881 aacgggaaga tttatgaaga aaggttttat gttgtttact ttgttagcgg cgttttcagg
    41941 ctttgctcag gctgatgacg cggcaattca acaaacgtta gccaaaatgg gcatcaaaag
    42001 cagcgatatt cagcccgcgc ccgtagctgg catgaagaca gttctgacta acagcggtgt
    42061 gttgtacatc accgatgatg gtaaacatat cattcagggg ccaatgtatg acgttagtgg
    42121 cacggctccg gtcaatgtca ccaataagat gctgttaaag cagttgaatg cgcttgaaaa
    42181 agagatgatc gtttataaag cgccgcagga aaaacacgtc atcaccgtgt ttactgatat
    42241 tacctgtggt tactgccaca aactgcatga gcaaatggca gactataacg cgctggggat
    42301 caccgtgcgt tatcttgctt tcccgcgcca ggggctggac agcgatgcag agaaagaaat
    42361 gaaagctatc tggtgtgcga aagataaaaa caaagcgttt gatgatgtga tggcaggtaa
    42421 aagcgtcgca ccagccagct gcgacgtgga tattgccgac cattacgcac ttggcgtcca
    42481 gcttggcgtt agcggtactc cggcagttgt gctgagcaat ggcacacttg ttccgggtta
    42541 ccagccgccg aaagacatga aagaattcct cgacgaacac caaaaaatga ccagcggtaa
    42601 ataattcgcg tgaaacaaca gatacaactt cgtcgccgtg aagtcgatga aacggcagac
    42661 ttgcccgctg aattgcctcc cttgctgcgc cgtttatacg ccagccgggg agtacgcagt
    42721 gcgcaagaac tggaacgcag tgttaaaggt atgttgccct ggcagcaact aagcggtgtc
    42781 gaaaaggccg ttgagatcct ttacaacgct tttcgcgaag gaacgcggat tattgtggtc
    42841 ggtgatttcg acgccgacgg cgcgaccagc acggctctaa gcgtgctggc gatgcgctcg
    42901 cttggttgca gcaatatcga ctacctggta ccaaaccgtt tcgaagacgg ttacggctta
    42961 agcccggaag tggtcgatca ggcccatgcc cgtggcgcgc agttaattgt cacggtggat
    43021 aacggtattt cctcccatgc gggagttgag cacgctcgct cgttgggcat cccggttatt
    43081 gttaccgatc accatttgcc gggcgacaca ttacccgcag cggaagcgat cattaaccct
    43141 aacttgcgcg actgtaattt cccgtcgaaa tcactggcag gcgtgggtgt ggcgttttat
    43201 ctgatgctgg cgctgcgcac ctttttgcgc gatcagggct ggtttgatga gcgcggcatc
    43261 gcaattccta acctggcaga actgctggat ctggtagcac tggggacagt agcggacgtc
    43321 gtgccgctgg acgctaataa tcgcattctg acctggcagg ggatgagtcg catccgtgcc
    43381 ggaaagtgcc gtccagggat taaagcgctg ctggaagtgg caaatcgcga tgcacaaaaa
    43441 ctagccgcca gcgatttagg ttttgcgctg ggaccgcgtc tcaatgctgc cgggcgactg
    43501 gatgatatgt ccgttggtgt ggcgctgttg ctgtgcgaca acatcggcga agcgcgcgtg
    43561 ctggcaaatg aactcgatgc gctaaaccag acgcgaaaag agatcgaaca aggaatgcag
    43621 gttgaagccc tgaccctgtg cgagaaactg gagcgcagcc gtgacacgct acccggcggg
    43681 ctggcaatgt atcaccccga atggcatcag ggcgttgtcg gtattctggc ttcgcgtatc
    43741 aaagagcgtt ttcaccgtcc ggttatcgcc tttgcgccag caggtgacgg tacgctgaaa
    43801 ggttccggtc gctccattca ggggctgcat atgcgtgatg cgctggagcg attagacaca
    43861 ctctaccccg gcatgatgct caagtttggc ggtcatgcga tggcggcggg tttgtcgctg
    43921 gaagaggata aattcgaact ctttcaacaa cggtttggcg aactggttac tgagtggctg
    43981 gccccttcgc tattgcaagg cgaagtggtg tcagacggtc cgttaagccc ggccgaaatg
    44041 accatggaag tggcgcagct gctgcgcgat gctggcccgt gggggcagat gttcccggag
    44101 ccgctgtttg acggtcattt ccgtctgctg caacagcggc tggtgggcga acgtcatttg
    44161 aaggtgatgg tcgaaccggt cggcggcggt ccactgctgg atggtattgc ttttaatgtc
    44221 gataccgctc tctggccgga taacggcgtg cgcgaagtgc aactggctta taagctcgat
    44281 atcaacgagt ttcgcggcaa ccgcagcctg caaattatca tcgacaatat ctggccaatt
    44341 tagcgtcatc ttctctataa aaaagagcgt ggattgggta caatcccgct cttatcaccg
    44401 cattttgact agctcaataa aagaaatcag accatgtttg aaattaatcc ggtaaataat
    44461 cgcattcagg acctcacgga acgctccgac gttcttaggg ggtatctttg actacgacgc
    44521 caagaaagag cgtctggaag aagtaaacgc cgagctggaa cagccggatg tctggaacga
    44581 acccgaacgc gcacaggcgc tgggtaaaga gcgttcctcc ctcgaagccg ttgtcgacac
    44641 cctcgaccaa atgaaacagg ggctggaaga tgtttctggt ctgctggaac tggctgtaga
    44701 agctgacgac gaagaaacct ttaacgaagc cgttgctgaa ctcgacgccc tggaagaaaa
    44761 actggcgcag cttgagttcc gccgtatgtt ctctggcgaa tatgacagcg ccgactgtta
    44821 cctcgatatt caggcggggt ctggcggtac ggaagcacag gactgggcga gcatgcttga
    44881 gcgtatgtat ctgcgctggg cagaatcgcg tggtttcaaa actgaaatca tcgaagagtc
    44941 ggaaggtgaa gtggcgggta ttaaatccgt gacgatcaaa atctccggcg attacgctta
    45001 cggctggctg cgtacagaaa ctggcgttca ccgcctggtg cgtaaaagcc cgtttgactc
    45061 cggcggtcgt cgccacacgt cgttcagctc tgcgtttgtt tatccggaag ttgatgatga
    45121 tattgatatc gaaatcaacc cggcggatct gcgcattgac gtttatcgcg cgtccggcgc
    45181 gggcggtcag cacgttaacc gtaccgaatc tgcggtgcgt attacccaca tcccgaccgg
    45241 gatcgtgacc cagtgccaga acgaccgttc ccagcacaag aacaaagatc aggccatgaa
    45301 gcagatgaaa gcgaagcttt atgaactgga gatgcagaag aaaaatgccg agaaacaggc
    45361 gatggaagat aacaaatctg acatcggctg gggcagccag attcgttctt atgtccttga
    45421 tgactcccgc attaaagatc tgcgcaccgg ggtagaaacc cgcaacacgc aggccgtgct
    45481 ggacggcagc ctggatcaat ttatcgaagc aagtttgaaa gcagggttat gaggaaccaa
    45541 catgtctgaa caacacgcac agggcgctga cgcggtagtc gatcttaaca atgaactgaa
    45601 aacgcgtcgt gagaagctgg cgaacctgcg tgagcaggga attgccttcc cgaacgattt
    45661 ccgtcgcgat catacctctg accaattgca cgcagaattc gacggtaaag agaacgaaga
    45721 actggaagcg ctgaacatcg aagtcgccgt tgctggccgc atgatgaccc gccgtattat
    45781 gggtaaagcg tctttcgtta ccctgcagga tgtgggtggc cgcattcagc tgtacgttgc
    45841 ccgtgacgat ctgccggaag gcgtttacaa cgagcagttc aaaaaatggg acctcggcga
    45901 catcctcggc gcgaaaggta aactgttcaa aaccaaaacc ggcgaactgt ctatccactg
    45961 taccgagctg cgtctgctga ccaaagcact gcgtccgctg ccggataaat tccacggctt
    46021 gcaggatcag gaagcgcgct atcgtcagcg ttatctggat ctcatctcta acgatgaatc
    46081 ccgtaacacc tttaaagtac gctcgcagat cctctctggt attcgccagt tcatggtgaa
    46141 ccgcggcttt atggaagttg aaacgccgat gatgcaggtg atcccaggcg gtgccgctgc
    46201 gcgtccgttt atcacccacc ataacgcgct ggatctcgac atgtacctgc gtatcgcgcc
    46261 ggaactgtac ctcaagcgtc tggtggtcgg tggcttcgag cgtgtattcg aaatcaaccg
    46321 taacttccgt aacgaaggta tttccgtacg tcataaccca gagttcacca tgatggaact
    46381 ctacatggct tacgcagatt acaaagatct gatcgagctg accgaatcgc tgttccgtac
    46441 tctggcacag gatattctcg gtaagacgga agtgacctac ggcgacgtga cgctggactt
    46501 cggtaaaccg ttcgaaaaac tgaccatgcg tgaagcgatc aagaaatatc gcccggaaac
    46561 cgacatggcg gatctggata acttcgactc tgcgaaagcg attgctgaat ctatcggtat
    46621 ccacgttgag aagagctggg gtctgggccg tatcgttacc gagatcttcg aagaagtggc
    46681 agaagcacat ctgattcagc cgaccttcat tactgaatat ccggcagaag tttctccgct
    46741 ggcgcgtcgt aacgacatta acccggaaat cacagaccgc tttgagttct tcatcggtgg
    46801 tcgtgaaatc ggtaacggct ttagcgagct gaatgacgcg gaagatcagg cgcaacgctt
    46861 cctggatcag gttgccgcga aagacgcagg tgacgacgaa gcgatgttct acgatgaaga
    46921 ttacgttacc gcactggaac atggcttacc gccgacagca ggtctgggga ttggcatcga
    46981 ccgtatggta atgctgttca ccaacagcca taccatccgc gacgttattc tgttcccggc
    47041 gatgcgtccg gtgaaataag cattacgtta tgctcacaac cccggcaaat gtcggggttt
    47101 tttatttaag ctgggtaaat gcagataatc gtattctggc ttcgcgattt gtcgcctgca
    47161 tcaccatcca cggactgaac gcccacggcg tggcatcaat accgcgtaat acatcagcta
    47221 aatcacacca ttgataatcc atcacttcat catcgttgat ctgtaacgca ctggtggtgc
    47281 gtgcggcaaa taccggacac acttcatttt ccacaatgcc tctcggatcg gtggcgcggt
    47341 agcgaaagtc aggatagata gattcaggag gcgtaatttc cacgccaagc tcataacggc
    47401 aacggcggat caccgcgtct tcgctgcttt ctcccagttg tgggtgccca caaaccgagt
    47461 tagtccacac gccaggccac gcttttttgc tcagtgcgcg gcgggtaact aataattgcc
    47521 ccttggcatt aaacagccaa ctggagaacg cgagatgtaa gagggtgtct gccgtgtgtg
    47581 cggcatactt ttccagcgta cccgtgggaa ctccctgtgc attcaataaa atgacgtgtt
    47641 ccgtttgcat aatttctcac atgtaattct gatcactcat cattatagat aattgaagag
    47701 tgaaatgatt aaccaagaga gcgtttatcg acaaccgccc gttttgagcg gatgccgaca
    47761 ggtaaaaacc atttaaaaaa gtagcatcat atatttgaag cgactttcaa tggccattgc
    47821 aacacagtca gaaatgacag gaatagagag agtaaaaagc aacaacagac tgaatatagc
    47881 gcgcattaaa aaattcatat tattctccat gctcattttt cttcagcttg cgcatcagaa
    47941 gccacatacg tactgtcctg acttctaaat ctttatcaga gacggattcc ttcaccaggt
    48001 cagcatgatg ctgatagcca ttaaagaaga cgttcagcac aacggcgcta agggttgcga
    48061 gcataatgcc gctatgtagc agcggttgca gtacggccgg taacttagaa aagaaatcgt
    48121 gagagagcgt cggagtcatg ccaacgccga gactgatcgc cacaatatag aggttgtaac
    48181 ggttggtggt gtagttacag cgcgacagaa ttcgaatccc tgtcgccagt accatgccga
    48241 acatcactag accagcgccg cccagcacaa attgcggaat ggaggctacc agcaccgcca
    48301 tttttggcac catgccaaac aggattaata taattcccga agaaatacac acccagcggc
    48361 tatgaacgcg catcacgcta accaggccaa cgttttgaga aaatgacgtg tgggggaagc
    48421 tgttaaacgt gccgcctatc attgtcccta cgccatcgac acgcagcccg caaataatat
    48481 cgtgtgaaga gagtttacga ccgactattt cacccagtgc caggaacatc cccatcgact
    48541 cgataaacac gatgattaac acggcagtca tggtcagaat ggaaacggga tcgaaaatcg
    48601 gcataccaaa cgacatcggg gtgacaatcg caaaccacga ggcatcatgt agcccggata
    48661 aattgacttc attcatcatc cacgaaagta aaaagccaaa tacaatcccc agtaatacgg
    48721 cgacgttgga cataaatcct ttcgcatagc gagtaatgag caagataaaa attaagacgg
    48781 caaaggagat acctaaataa acgggattac catattgcgg attcccttta cctccagcgg
    48841 cccagtcaat acccacctga atgatgctaa gcccaataga agtaataacc acaccggtaa
    48901 ccagtggcgg gaataaaggc atcaagcgac caataagtgg cgctaataat gtggtgataa
    48961 aacccgcggc gatagtggca ccaaatatcc ccagcaggcc gatatccggg ttcatcccaa
    49021 tggctatcat tggtgttaca gcagcaaagg tcaccgacat aatcaccggc aggcggatcc
    49081 ccataaagcg gccgataccg atacattgca ataatgtgac gatcccgcag caaaagagat
    49141 ccgagctaat gagcatcgca atagcatctt ttgagaggcc cagtcggtca ccaatcataa
    49201 gaggaacagc gactgcacct gcgtacatga caaggacgtg ttgcagaccg agtatgatca
    49261 gctttcctgg tgataatatg cggtcaacct catcagttgg gcggtcttgc ccagaagatg
    49321 agggaagttg ggaatctatg gcgctcatca gtgtttctcc tttcgcccgc cagaataaaa
    49381 agcaaccgtt tacgttaatg aaactgtcta aacacatgct cgtggagtgc cagtttttgc
    49441 gaggaagggg agttagagac aggaattgcg gaggcaatca cataatcgtg cttaaaccac
    49501 gaaaatgaca agagaaactg acaa
//
LOCUS       DAJTRC010000019        49088 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.019, whole genome shotgun sequence.
ACCESSION   DAJTRC010000019 DAJTRC010000000
VERSION     DAJTRC010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 49088)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49088)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49088
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.019"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..116
                     /locus_tag="OO357_001201"
     CDS             <1..116
                     /locus_tag="OO357_001201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5886216.1"
                     /translation="NLRQHLARLGRKSLSFSKSVELYDKVIGHYLNIKHYQ"
     gene            complement(110..1288)
                     /gene="setB"
                     /locus_tag="OO357_001202"
     CDS             complement(110..1288)
                     /gene="setB"
                     /locus_tag="OO357_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000551951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar efflux transporter SetB"
                     /protein_id="HCR5886217.1"
                     /translation="MHNSPAVSSAKSFDLTSTAFLIVAFLTGIAGALQTPTLSIFLTD
                     EVHARPAMVGFFFTGSAVIGILVSQFLAGRSDKRGDRKSLIVFCCLLGVLACTLFAWN
                     RNYFVLLFVGVFLSSFGSTANPQMFALAREHADKTGREAVMFSSFLRAQVSLAWVIGP
                     PLAYALAMGFSFTVMYLSAAVAFIVCGVMVWLFLPSMQKELPLATGTVEAPRRNRRDT
                     LLLFVICTLMWGSNSLYIINMPLFIINELHLPEKLAGVMMGTAAGLEIPTMLIAGYFA
                     KRLGKRFLMRVAAVGGVCFYAGMLMAHSPAILLGLQLLNAIFIGILGGIGMLYFQDLM
                     PGQAGSATTLYTNTSRVGWIIAGSVAGIVAEIWNYHAVFWFAMVMIIATLFWVMTPTY
                     "
     gene            1656..2786
                     /gene="fruB"
                     /locus_tag="OO357_001203"
     CDS             1656..2786
                     /gene="fruB"
                     /locus_tag="OO357_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused PTS fructose transporter subunit IIA/HPr
                     protein"
                     /protein_id="HCR5886218.1"
                     /translation="MFQLSVQDIHPGEKAGDKEEAIRQVAAALVQAGNVAEGYVNGML
                     AREQQTSTFLGNGIAIPHGTTDTRDQVLKTGVQVFQFPEGVTWGDGQVAYVAIGIAAS
                     SDKHLGLLRQLTHVLSDDSVAEQLKSATTAEELRALLMGEKQSEQLKLDNEMLTLDIV
                     ASDLLTLQALNAARLKEARAVDATFVTKAINEQPLNLGQGIWLSDSAEGNLRSAIAVS
                     RAANAFDVDGETAAMLVSVAMNDDQPIAVLKRLADLLLDNKADRLLKADAATLLALLT
                     SDDAPTDDVLSAEFVVRNEHGLHARPGTMLVNTIKQFNSDITVTNLDGTGKPANGRSL
                     MKVVALGVKKGHRLRFTAQGADAEQALKAIGDAIAAGLGEGA"
     gene            2786..3724
                     /gene="fruK"
                     /locus_tag="OO357_001204"
     CDS             2786..3724
                     /gene="fruK"
                     /locus_tag="OO357_001204"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HCR5886219.1"
                     /translation="MSRRVATITLNPAYDLVGFCPEIERGEVNLVKTTGLHAAGKGIN
                     VAKVLKDLGIDVTVGGFLGKDNQDGFQQLFSELGIANRFQVVQGRTRINVKLTEKDGE
                     VTDFNFSGFEVTPADWERFVTDSLSWLGQFDMVCVSGSLPSGVSPEAFTDWMTRLRSQ
                     CPCIIFDSSREALVAGLKAAPWLVKPNRRELEIWAGRKLPEMKDVIEAAHALREQGIA
                     HVVISLGAEGALWVNASGEWIAKPPSVDVVSTVGAGDSMVGGLIYGLLMRESSEHTLR
                     LATAVAALAVSQSNVGITDRPQLAAMMARVDLQPFN"
     gene            3741..5432
                     /gene="fruA"
                     /locus_tag="OO357_001205"
     CDS             3741..5432
                     /gene="fruA"
                     /locus_tag="OO357_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIBC"
                     /protein_id="HCR5886220.1"
                     /translation="MKTLLIIDANLGQARAYMAKTLLGAAARKAKLEIIDNPNDAEMA
                     IVLGDSIPNDSALNGKNVWLGDISRAVAHPELFLSEAKGHAKPYTAPVTATAPVAASG
                     PKRVVAVTACPTGVAHTFMAAEAIETEAKKRGWWVKVETRGSVGAGNAITPEEVAAAD
                     LVIVAADIEVDLAKFAGKPMYRTSTGLALKKTSQELDKAVAEATPYEPAGKAQTATTE
                     GKKESAGAYRHLLTGVSYMLPMVVAGGLCIALSFAFGIEAFKEPGTLAAALMQIGGGS
                     AFALMVPVLAGYIAFSIADRPGLTPGLIGGMLAVSTGSGFIGGIIAGFLAGYIAKLIS
                     TQLKLPQSMEALKPILIIPLISSLVVGLAMIYLIGKPVAGILEGLTHWLQTMGTANAV
                     LLGAILGGMMCTDMGGPVNKAAYAFGVGLLSTQTYGPMAAIMAAGMVPPLAMGLATMV
                     ARRKFDKAQQEGGKAALVLGLCFISEGAIPFAARDPMRVLPCCIVGGALTGAISMAIG
                     AKLMAPHGGLFVLLIPGAITPVLGYLVAIIAGTLVAGLAYAFLKRPEVDAVAKAA"
     gene            5856..6797
                     /locus_tag="OO357_001206"
     CDS             5856..6797
                     /locus_tag="OO357_001206"
                     /EC_number="2.7.1.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine kinase"
                     /protein_id="HCR5886221.1"
                     /translation="MREKDYVVIIGSANIDVAGYSHESLNYADSNPGKIKFTPGGVGR
                     NIAQNLALLGNKAWLLSAVGSDFYGQSLLTQTNQSGVYVDKCLIVPGENTSSYLSLLD
                     NTGEMLVAINDMNISNAITAEYLAQHREFIQRAKVIVADCNISEEALAWILDNAANVP
                     VFVDPVSAWKCVKVRDRLNQNHTLKPNRLEAETLSGIALSGRDDVAKVAAWFHQHGLN
                     RLVLSMGGDGVYYSDISGENGWSAPIKTNVINVTGAGDAMMAGLASCWVDGMPFAESV
                     RFAQGCSSMALSCEYTNNPDLSIANVISLVENAECLN"
     gene            6785..7723
                     /gene="psuG"
                     /locus_tag="OO357_001207"
     CDS             6785..7723
                     /gene="psuG"
                     /locus_tag="OO357_001207"
                     /EC_number="4.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HCR5886222.1"
                     /translation="MSELKISPELLQISPEVQDALKNKKPVVALESTIISHGMPFPQN
                     AQTAIEVEETIRKQGAVPATIAIIGGVMKVGLSKEKIELLGREGHNVTKVSRRDLPFV
                     VAAGKNGATTVASTMIIAALAGIKVFATGGIGGVHRGAEHTFDISADLQELANTNVTV
                     VCAGAKSILDLGLTTEYLETFGVPLIGYQTKALPAFFCRTSSFDVSIRLDSASEIARA
                     MAVKWQSGLNGGLVVANPIPEQFAMPEESINAAIDQAVAEAEEQGVIGKESTPFLLAR
                     VAELTSGDSLKSNIQLVFNNAILASEIAKEYQRLAG"
     gene            7817..9067
                     /locus_tag="OO357_001208"
     CDS             7817..9067
                     /locus_tag="OO357_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5886223.1"
                     /translation="MDIMRSVVGMVVLLAIAFLLSVNKKSISLRTVGAALLLQIAIGG
                     IMLYFPPGKWAVEQAALGVHKVMSYSDAGSAFIFGSLVGPKMDVLFDGAGFIFAFRVL
                     PAIIFVTALISLLYYIGVMGLLIRILGSIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRVNRNELFTAICSGMASIAGSVMIGYAGMGVPIDYLLAASLMAIPGGILFAR
                     ILSPATEPSQVTFENLSFSETPPKSFIEAAASGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGIGGWFGFANASLESIFGYVLAPLAWIMGVDWSDANLAGSLIGQKLAINEFVA
                     YLSFSPYLQTGGTLEVKTVAIISFALCGFANFGSIGVVVGAFSAISPKRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(9138..9832)
                     /gene="yeiL"
                     /locus_tag="OO357_001209"
                     /pseudo
     CDS             complement(9138..9832)
                     /gene="yeiL"
                     /locus_tag="OO357_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311082.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator YeiL"
     gene            9963..10904
                     /gene="rihB"
                     /locus_tag="OO357_001210"
     CDS             9963..10904
                     /gene="rihB"
                     /locus_tag="OO357_001210"
                     /EC_number="3.2.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosylpyrimidine nucleosidase"
                     /protein_id="HCR5886224.1"
                     /translation="MEKRKIILDCEPGHDDAIAMMMAAKHPAIDLLGITIVAGNQTLD
                     KTLINGLNVCQKLEINVPVYAGMPQPIMRKQIVADNIHGETGLDGPVFEPLTRQAEST
                     HAVKYIIDTLMASDGDITLVPVGPLSNIAVAMRMQPAILPKIREIVLMGGAYGTGNFT
                     PSAEFNIFADPEAARVVFTSGVPLVMMGLDLTNQTVCTPDVIARMERVGGPAGELFSD
                     IMNFTLKTQFEYYGLAGGPVHDATCIGYLINPDGIKTQDMYVEVDVNSGPCYGRTVCD
                     ELGVLGKPANTKVGITIDTDWFWGLVEECVRGYIKTH"
     gene            11004..12254
                     /locus_tag="OO357_001211"
     CDS             11004..12254
                     /locus_tag="OO357_001211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HCR5886225.1"
                     /translation="MDVMRSVLGMVVLLAIAFLLSVNKKKISLRTVGAALVLQVVIGG
                     IMLWLPPGRWVAEKVAFGVHKVMAYSDAGSAFIFGSLVGPKMDTLFDGAGFIFGFRVL
                     PAIIFVTALVSILYYIGVMGILIRILGGIFQKALNISKIESFVAVTTIFLGQNEIPAI
                     VKPFIDRLNRNELFTAICSGMASIAGSTMIGYAALGVPVEYLLAASLMAIPGGILFAR
                     LLSPATESSQVSFNNLSFTETPPKSIIEADATGAMTGLKIAAGVATVVMAFVAIIALI
                     NGIIGGVGGWFGFEHASLESILGYLLAPLAWVMGVDWSDANLAGSLIGQKLAINEFVA
                     YLNFSPYLQTAGTLDAKTVAIISFALCGFANFGSIGVVVGAFSAVAPHRAPEIAQLGL
                     RALAAATLSNLMSATIAGFFIGLA"
     gene            complement(12310..13398)
                     /locus_tag="OO357_001212"
     CDS             complement(12310..13398)
                     /locus_tag="OO357_001212"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HCR5886226.1"
                     /translation="MNNREKEILAILRRNPLIQQNEIADMLQISRSRVAAHIMDLMRK
                     GRIKDKGYILTEQEYCVVVGTINMDIRGMADIHYPQAASHPGTIHCSAGGVGRNIAHN
                     LALLGRDVHLLSVIGDDFYGEMLLEETRRAGVNVSGCVRLHGQSTSTYLAIANRDDQT
                     VLAINDTHLLEQLTPQLLNGSRDLLRHAGVVLADCNLTAEALEWVFTLADEIPVFVDT
                     VSEFKAGKIKHWLAHIHTLKPTLPELEILWGQAITSDADRNAAVNALHQQGVQQLFVY
                     LPDESVYCSEKDGEQFLLTVPAHTTVDSFGADDGFMAGLVYSFLEGNNFRDSARFAMA
                     CAAISRASGSLNNPTLSADNALSLVPMV"
     gene            complement(13401..14258)
                     /gene="nfo"
                     /locus_tag="OO357_001213"
     CDS             complement(13401..14258)
                     /gene="nfo"
                     /locus_tag="OO357_001213"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HCR5886227.1"
                     /translation="MKYIGAHVSAAGGLANAAIRAAEIDATAFALFTKNQRQWRAAPL
                     TTQTIDEFKAACEKYHYTSAQILPHDSYLINLGHPVTEALEKSRDAFIDEMQRCEQLG
                     LSLLNFHPGSHLMQISEEDCLARIAESINIALDKTQGVTAVIENTAGQGSNLGFKFEH
                     LAAIIDGVEDKSRVGVCIDTCHAFAAGYDLRTPAECEKTFADFARIVGFKYLRGMHLN
                     DAKSTFGSRVDRHHSLGEGNIGHDAFRWIMQNDRFDGIPLILETINPDIWAEEIAWLK
                     AQQTEKAVA"
     gene            complement(14332..15381)
                     /locus_tag="OO357_001214"
     CDS             complement(14332..15381)
                     /locus_tag="OO357_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeiH family protein"
                     /protein_id="HCR5886228.1"
                     /translation="MTNITLQKQHRTLWHFIPGLALSAVITGVALWGGSIPAVAGAGF
                     SALTLAILLGMVLGNTIYPHIWKSCDGGVLFAKQYLLRLGIILYGFRLTFSQIADVGI
                     SGIIIDVLTLSSTFLLACFLGQKVFGLDKHTSWLIGAGSSICGAAAVLATEPVVKAEA
                     SKVTVAVATVVIFGTVAIFLYPAIYPLMSQWFSPETFGIYIGSTVHEVAQVVAAGHAI
                     SPDAENAAVISKMLRVMMLAPFLILLAARVKQLSGANSGEKSKITIPWFAILFIVVAI
                     FNSFHLLPQSVVNMLVTLDTFLLAMAMAALGLTTHVSALKKAGAKPLLMALVLFAWLI
                     VGGGAINYVIQSVIA"
     gene            15480..16361
                     /locus_tag="OO357_001215"
     CDS             15480..16361
                     /locus_tag="OO357_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5886229.1"
                     /translation="MHITLRQLEVFAEVLKSGSTTQASVMLALSQSAVSAALTDLEGQ
                     LGVQLFDRVGKRLVVNEHGRLLYPRALALLEQAVEIEQLFREDNGAIRIYASSTIGNY
                     ILPAVIARYRHDYPQLPIELSVGNSQDVMQAVLDFRVDIGFIEGPCHSTEIISEPWLE
                     DELVVFAAPTSPLARGPVTLEQLAAAPWILRERGSGTRDIVDYLLLSHLPKFEMAMEL
                     GNSEAIKHAVRHGLGISCLSRRVIEDQLQAGTLSEVAVPLPRLMRTLWRIHHRQKHLS
                     NALRRFLDYCDPANVPR"
     gene            16567..18036
                     /gene="lysP"
                     /locus_tag="OO357_001216"
     CDS             16567..18036
                     /gene="lysP"
                     /locus_tag="OO357_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-specific permease"
                     /protein_id="HCR5886230.1"
                     /translation="MGSETKTTEAPGLRRELKARHLTMIAIGGSIGTGLFVASGATIS
                     QAGPGGALLSYMLIGLMVYFLMTSLGELAAYMPVSGSFATYGQNYVEEGFGFALGWNY
                     WYNWAVTIAVDLVAAQLVMSWWFPDTPGWIWSALFLGVIFLLNYISVRGFGEAEYWFS
                     LIKVTTVIVFIIVGVLMIIGIFKGAQPAGWSNWTIGEAPFAGGFAAMIGVAMIVGFSF
                     QGTELIGIAAGESEDPAKNIPRAVRQVFWRILLFYVFAILIISLIIPYTDPSLLRNDV
                     KDISVSPFTLVFQHAGLLSAAAVMNAVILTAVLSAGNSGMYASTRMLYTLACDGKAPR
                     IFAKLSRGGVPRNALYATTVIAGLCFLTSMFGNQTVYLWLLNTSGMTGFIAWLGIAIS
                     HYRFRRGYVLQGHDINDLPYRSGFFPLGPIFAFILCLIITLGQNYEAFLKDTIDWGGV
                     AATYIGIPLFLIIWFGYKLIKGTHFVRYSEMKFPQNDKK"
     gene            18328..20319
                     /gene="cirA"
                     /locus_tag="OO357_001217"
                     /pseudo
     CDS             18328..20319
                     /gene="cirA"
                     /locus_tag="OO357_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416660.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor CirA"
     gene            complement(20351..21186)
                     /gene="yeiG"
                     /locus_tag="OO357_001218"
                     /pseudo
     CDS             complement(20351..21186)
                     /gene="yeiG"
                     /locus_tag="OO357_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="S-formylglutathione hydrolase"
     gene            21444..22112
                     /gene="folE"
                     /locus_tag="OO357_001219"
     CDS             21444..22112
                     /gene="folE"
                     /locus_tag="OO357_001219"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HCR5886231.1"
                     /translation="MPSLSKEAALVHEALVARGLETPLRPPVHEMDNETRKSLIAGHM
                     TEIMQLLNLDLADDSLMETPHRIAKMYVDEIFSGLDYANFPKITLIENKMKVDEMVTV
                     RDITLTSTCEHHFVTIDGKATVAYIPKDSVIGLSKINRIVQFFAQRPQVQERLTQQIL
                     IALQTLLGTNNVAVSIDAVHYCVKARGIRDATSATTTTSLGGLFKSSQNTRHEFLRAV
                     RHHN"
     gene            22129..23205
                     /locus_tag="OO357_001220"
     CDS             22129..23205
                     /locus_tag="OO357_001220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF418 family protein"
                     /protein_id="HCR5886232.1"
                     /translation="MERNVTLDFVRGVAILGILLLNISAFGLPKAAYLNPAWYDAITP
                     QDAWTWAFLDLIGQVKFLTLFALLFGAGLQMLLPRGRRWIQSRLTLLVLLGFIHGLLF
                     WDGDILLAYGLVGLGVLLLLGLISDSQTSRAWTPDASAILYEKYWKLHGGVEAISNRA
                     DGVGNSLLALGAQYGWQLAGMMLIGAALMRSGWLKGQFSLRHYRRTGFVLVAIGVTIN
                     LPAIALQWQLDWAYRWCAFLLQMPRELSAPFQAIGYASLFYGFWPQLSRFKLVLAIAC
                     VGRMALTNYLLQTLICTTLFYHLGLFMHFDRLELLAFVIPVWLANILFSVIWLRYFRQ
                     GPVEWLWRQLTLRAAGPAISKTSR"
     gene            23347..24387
                     /gene="galS"
                     /locus_tag="OO357_001221"
     CDS             23347..24387
                     /gene="galS"
                     /locus_tag="OO357_001221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708050.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalS"
                     /protein_id="HCR5886233.1"
                     /translation="MITIRDVARQAGVSVATVSRVLNNSTLVSADTREAVMKAVSELD
                     YRPNANAQALATQVSDTIGVVVMDVSDAFFGALVKAVDLVAQQHQKYVLIGNSYHEAE
                     KERHAIEVLIRQRCNALIVHSKALSDDELAQFMDNIPGMVLINRVVPGYAHRCVCLDN
                     LSGARMATRMLLNNGHQRIGYLSSSHGIEDDAMRKAGWMSALKEQDIIPPESWIGTGT
                     PDMPGGEAAMVELLGRNLQLTAVFAYNDNMAAGALTALKDNGIAIPLHLSIIGFDDIP
                     IARYTDPQLTTVRYPIASMAKLATELALQGAAGNIDPRASHCFMPTLVRRHSVATRQN
                     AAAITNSTNQAM"
     gene            24667..25665
                     /gene="mglB"
                     /locus_tag="OO357_001222"
     CDS             24667..25665
                     /gene="mglB"
                     /locus_tag="OO357_001222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/glucose ABC transporter
                     substrate-binding protein MglB"
                     /protein_id="HCR5886234.1"
                     /translation="MNKKVLTLSAVMASMLFGAAAHAADTRIGVTIYKYDDNFMSVVR
                     KAIEQDAKAAPDVQLLMNDSQNDQSKQNDQIDVLLAKGVKALAINLVDPAAAGTVIEK
                     ARGQNVPVVFFNKEPSRKALDSYDKAYYVGTDSKESGIIQGDLIAKHWAANQGWDLNK
                     DGQIQFVLLKGEPGHPDAEARTTYVIKELNDKGIKTEQLQLDTAMWDTAQAKDKMDAW
                     LSGPNANKIEVVIANNDAMAMGAVEALKAHNKSSIPVFGVDALPEALALVKSGALAGT
                     VLNDANNQAKATFDLAKNLADGKGAADGTNWKIDNKVVRVPYVGVDKDNLAEFSKK"
     gene            25726..27246
                     /gene="mglA"
                     /locus_tag="OO357_001223"
     CDS             25726..27246
                     /gene="mglA"
                     /locus_tag="OO357_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020883197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     ATP-binding protein MglA"
                     /protein_id="HCR5886235.1"
                     /translation="MVSSTTPSSGEYLLEMSGINKSFPGVKALDNVNLKVRPHSIHAL
                     MGENGAGKSTLLKCLFGIYQKDSGTILFQGKEIDFHSAKEALENGISMVHQELNLVLQ
                     RSVMDNMWLGRYPTKGMFVDQDKMYRETKAIFDELDIDIDPRARVGTLSVSQMQMIEI
                     AKAFSYNAKIVIMDEPTSSLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDE
                     VTVLRDGQWIATEPLAGLTMDKIIAMMVGRSLNQRFPDKENKPGEVILEVRNLTSLRQ
                     PSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFGIREKSAGTITLHGKQINNHNANE
                     AINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYKNKVGLLDNSRMKSDTQWVIDS
                     MRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLDEPTRGIDVGAKFEIYQLIA
                     ELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTKTTTQNEILRLASLHL"
     gene            27262..28272
                     /gene="mglC"
                     /locus_tag="OO357_001224"
     CDS             27262..28272
                     /gene="mglC"
                     /locus_tag="OO357_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/methyl galactoside ABC transporter
                     permease MglC"
                     /protein_id="HCR5886236.1"
                     /translation="MSALNKKSFLTYLKEGGIYVVLLVLLAIIIFQDPTFLSLLNLSN
                     ILTQSSVRIIIALGVAGLIVTQGTDLSAGRQVGLAAVVAATLLQSMDNANKVFPEMAT
                     MPIALVILIVCAIGAVIGLINGLIIAYLNVTPFITTLGTMIIVYGINSLYYDFVGASP
                     ISGFDSGFSTFAQGFVALGSFRLSYITFYALIAVAFVWVLWNKTRFGKNIFAIGGNPE
                     AAKISGVNVGLNLLMIYALSGVFYAFGGMLEAGRIGSATNNLGFMYELDAIAACVVGG
                     VSFSGGVGTVIGVVTGVIIFTVINYGLTYIGVNPYWQYIIKGAIIIFAVALDSLKYAR
                     KK"
     gene            complement(28308..29521)
                     /gene="preA"
                     /locus_tag="OO357_001225"
                     /pseudo
     CDS             complement(28308..29521)
                     /gene="preA"
                     /locus_tag="OO357_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416652.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
     gene            complement(29515..30753)
                     /gene="preT"
                     /locus_tag="OO357_001226"
     CDS             complement(29515..30753)
                     /gene="preT"
                     /locus_tag="OO357_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreT"
                     /protein_id="HCR5886237.1"
                     /translation="MPQQNYLDELTPAFTPLLAIKEASRCLLCHDAPCSQACPAQTDP
                     GKFIRSIYFRNFKGAAETIRENNALGAVCARVCPTEKLCQSGCTRAGVDAPIDIGRLQ
                     RFVTDFEQQTGMEIYQPGTKTLGKVAIIGAGPAGLQASVTLTNQGYDVTIYEKEAHPG
                     GWLRNGIPQFRLPQSVLDAEIARIEKMGVTIKCNNEIGKTLTLEQLKAENRAVLVTVG
                     LSSGSGLPLFEHSDVEIAVDFLQRVRQAQGDISIPQSALIIGGGDVAMDVASTLKVLG
                     CQAVTCVAREELDEFPASEKEFASARELGVSIIDGFTPVAVEGNKVTFKHVRLSGELT
                     MAADKIILAVGQHARLDAFAELEQQRNTIKTQNYQIRDPQVFAAGDIVEGDKTVVYAV
                     KTGKEAAEAIHHYLEGACSC"
     gene            complement(30947..31186)
                     /locus_tag="OO357_001227"
     CDS             complement(30947..31186)
                     /locus_tag="OO357_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2542 family protein"
                     /protein_id="HCR5886238.1"
                     /translation="MDVQQFFVVAVFFLIPIFCFREAWKGWRAGAIDKRVKNAPEPVY
                     VWRAKNPGLFFAYMVAYIGFGILSIGMIVYLIFYR"
     gene            complement(31189..31908)
                     /gene="sanA"
                     /locus_tag="OO357_001228"
     CDS             complement(31189..31908)
                     /gene="sanA"
                     /locus_tag="OO357_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane permeability protein SanA"
                     /protein_id="HCR5886239.1"
                     /translation="MLKRVFLSLLVLIGLLLLTVLGLDRWMSWKTAPYIYDELQDLPY
                     RQVGVVLGTAKYYRTGVINQYYRYRIQGAINAYNSGKVNYLLLSGDNALQSYNEPMTM
                     RKDLIAAGVDPSDIVLDYAGFRTLDSIVRTRKVFDTNDFIIITQRFHCERALFIALHM
                     GIQAQCYAVPSPKDMLSVRIREFAARFGALADLYIFKREPRFLGPLVPIPAMHQVPED
                     AQGYPAVTPEQLLELQKKQGK"
     gene            complement(32058..32942)
                     /gene="cdd"
                     /locus_tag="OO357_001229"
     CDS             complement(32058..32942)
                     /gene="cdd"
                     /locus_tag="OO357_001229"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HCR5886240.1"
                     /translation="MHPRFQTAFAQLADNLQSALEPILADKYFPALLTGEQVSSLKSA
                     TGLDEDALAFALLPLAAACARTPLSNFNVGAIARGVSGTWYFGANMEFIGATMQQTVH
                     AEQSAISHAWLSGEKALAAITVNYTPCGHCRQFMNELNSGLDLRIHLPGREAHALRDY
                     LPDAFGPKDLEIKTLLMDEQDHGYALTGDALSQAAIAAANRSHMPYSKSPSGVALECK
                     DGRIFSGSYAENAAFNPTLPPLQGALILLNLKGYDYPDIQRAVLAEKADAPLIQWDAT
                     SATLKALGCHSIDRVLLA"
     gene            complement(33072..33767)
                     /locus_tag="OO357_001230"
     CDS             complement(33072..33767)
                     /locus_tag="OO357_001230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidB/LrgB family autolysis modulator"
                     /protein_id="HCR5886241.1"
                     /translation="MMANIWWSLPLTLIVFFAARKLAARYKFPLLNPLLVAMVVIIPF
                     LMLTGISYDSYFKGSEVLNDLLQPAVVALAYPLYEQLHQIRARWKSIITICFIGSVVA
                     MVTGTSVALLMGASPEIAASILPKSVTTPIAMAVGGSIGGIPAISAVCVIFVGILGAV
                     FGHTLLNAMRIRTKAARGLAMGTASHALGTARCAELDYQEGAFSSLALVLCGIITSLI
                     APFLFPIILAVMG"
     gene            complement(33764..34162)
                     /locus_tag="OO357_001231"
     CDS             complement(33764..34162)
                     /locus_tag="OO357_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HCR5886242.1"
                     /translation="MSKTLNIIWQYLRAFVLIYACLYAGIFIASLLPVTIPGSIIGML
                     ILFVLLALQILPAKWVNPGCYVLIRYMALLFVPIGVGVMQYFDLLRAQFGPVVVSCAV
                     STLVVFLVVSWSSQLVHGERKVVGQKGSEE"
     gene            34401..35348
                     /gene="dusC"
                     /locus_tag="OO357_001232"
     CDS             34401..35348
                     /gene="dusC"
                     /locus_tag="OO357_001232"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(16) synthase DusC"
                     /protein_id="HCR5886243.1"
                     /translation="MRVLLAPMEGVLDSLVRELLTEVNDYDLCITEFVRVVDQLLPVK
                     VFHRICPELQNASRTPSGTLVRVQLLGQFPQWLAENAARAVELGFWGVDLNCGCPSKT
                     VNGSGGGATLLKDPELIYQGAKAMREAVPAHLPVSVKVRLGWDSGEKKFEIADAVQQA
                     GATELVVHGRTKEQGYRAEHIDWQAIGEIRQRLNIPVIANGEIWDWQSAQQCMAISGC
                     DAVMIGRGALNIPNLSRVVKYNEPRMPWPEVVALLQKYTRLEKQGDTGLYHVARIKQW
                     LSYLRKEYDEATELFQHVRVLNNSPDIARAIQAIDIEKL"
     gene            complement(35719..35802)
                     /gene="yohP"
                     /locus_tag="OO357_001233"
     CDS             complement(35719..35802)
                     /gene="yohP"
                     /locus_tag="OO357_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YohP"
                     /protein_id="HCR5886244.1"
                     /translation="MKIILWAVLIIFLIGLLVVTGVFKMIF"
     gene            36026..37462
                     /gene="mdtQ"
                     /locus_tag="OO357_001234"
                     /pseudo
     CDS             36026..37462
                     /gene="mdtQ"
                     /locus_tag="OO357_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001583680.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug resistance outer membrane protein
                     MdtQ"
     gene            37515..38276
                     /locus_tag="OO357_001235"
                     /pseudo
     CDS             37515..38276
                     /locus_tag="OO357_001235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416641.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(38393..38971)
                     /locus_tag="OO357_001236"
     CDS             complement(38393..38971)
                     /locus_tag="OO357_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5886245.1"
                     /translation="MDLNTLISQYGYAALVIGSLAEGETVTLLGGVAAHQGLLKFPLV
                     VLSVALGGMIGDQVLYLCGRRFGGKLLRRFSKHQDKIERAQKLIQRHPYLFVIGTRFM
                     YGFRVIGPTLIGASQLPPKIFLPLNILGAFAWALIFTTIGYAGGQVIAPWLHNLDQHL
                     KHWVWLILVVVLVVGVRWWLKRRGKKKPDNQA"
     gene            39141..39728
                     /locus_tag="OO357_001237"
     CDS             39141..39728
                     /locus_tag="OO357_001237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014170891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HCR5886246.1"
                     /translation="MSHVWGLFSHPDREMQVINRENETISHHYTHHVLLMAAIPVICA
                     FIGTTQIGWNFGDGTILKLSWFTGLALAVLFYGVMLAGVAVMGRVIWWMARNYPQRPS
                     LAHCMVFAGYVATPLFLSGLVALYPLVWLCALVGTVALFYTGYLLYLGIPSFLNINKE
                     EGLSFSSSTLAIGVLVLEVLLTLTVILWGYGYRLF"
     gene            39902..40834
                     /gene="pbpG"
                     /locus_tag="OO357_001238"
     CDS             39902..40834
                     /gene="pbpG"
                     /locus_tag="OO357_001238"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine endopeptidase"
                     /protein_id="HCR5886247.1"
                     /translation="MPKFRVSLFSLALMLAVPFAPQAVAKTAAATTASQPEIASGSAM
                     IVDLNTNKVIYSNHPDLVRPIASISKLMTAMVVLDARLPLDEKLKVDISQTPEMKGVY
                     SRVRLNSEISRKDMLLLALMSSENRAAASLAHHYPGGYKAFIKAMNAKAKSLGMNNTR
                     FVEPTGLSVHNVSTARDLTKLLIASKQYPLIGQLSTTREDMATFSNPTYTLPFRNTNH
                     LVYRDNWNIQLTKTGFTNAAGHCLVMRTVINNKPVALVVMDAFGKYTHFADASRLRTW
                     IETGKVMPVPAAALSYKKQKAAQMAAAGQTAQND"
     gene            complement(40872..42587)
                     /gene="dld"
                     /locus_tag="OO357_001239"
     CDS             complement(40872..42587)
                     /gene="dld"
                     /locus_tag="OO357_001239"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5886248.1"
                     /translation="MSSMTTTDNKAFLNELARLVGHSHLLTDPAKTARYRKGFRSGQG
                     DALAVVFPGSLLELWRVLKACVTADKIILMQAANTGLTEGSTPNGNDYDRDIVIISTL
                     RLDKLHVLGKGEQVLAYPGTTLYSLEKALKPLGREPHSVIGSSCIGASVIGGICNNSG
                     GSLVQRGPAYTEMSLFARIDEDGKLTLVNHLGIDLGETPEQILSKLDDDRIKDDDVRH
                     DGRHAHDYDYVHRVRDIEADTPARYNADPDRLFESSGCAGKLAVFAVRLDTFEAEKNQ
                     QVFYIGTNQPEVLTEIRRHILANFENLPVAGEYMHRDIYDIAEKYGKDTFLMIDKLGT
                     DKMPFFFNLKGRTDAMLEKVKFFRPHFTDRAMQKFGHLFPSHLPPRMKNWRDKYEHHL
                     LLKMAGDGVGEAKSWLVDYFKQAEGDFFVCTPEEGSKAFLHRFAAAGAAIRYQAVHSD
                     EVEDILALDIALRRNDTEWYEHLPPEIDSQLVHKLYYGHFMCYVFHQDYIVKKGVDVH
                     ALKEQMLELLQQRGAQYPAEHNVGHLYKAPETLQKFYRENDPTNSMNPGIGKTSKRKN
                     WQEVE"
     gene            42783..45080
                     /gene="bglX"
                     /locus_tag="OO357_001240"
     CDS             42783..45080
                     /gene="bglX"
                     /locus_tag="OO357_001240"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucosidase BglX"
                     /protein_id="HCR5886249.1"
                     /translation="MKWLCSVGIAVSLVLQPALADDLFGNHPLTPEARDAFVTDLLKK
                     MTVDEKIGQLRLISVGPDNPKEAIREMIKDGQVGAIFNTVTRQDIRAMQDQVMELSRL
                     KIPLFFAYDVLHGQRTVFPISLGLASSFNLDAVKTVGRVSAYEAADDGLNMTWAPMVD
                     VSRDPRWGRASEGFGEDTYLTSIMGKTMVEAMQGKSPADRYSVMTSVKHFAAYGAVEG
                     GKEYNTVDMSPQRLFNDYMPPYKAGLDAGSGAVMVALNSLNGTPATSDSWLLKDVLRD
                     QWGFKGITVSDHGAIKELIKHGTAADPEDAVRVALKSGINMSMSDEYYSKYLPGLIKS
                     GKVTMAELDDAARHVLNVKYDMGLFNDPYSHLGPKESDPVDTNAESRLHRKEAREVAR
                     ESLVLLKNRLETLPLKKSATIAVVGPLADSKRDVMGSWSAAGVADQSVTVLTGIKNSV
                     GENGKVLYAKGANVTSDKGIIDFLNQYEEAVKVDPRSPQEMIDEAVQTAKQSDVVVAV
                     VGEAQGMAHEASSRTDITIPQGQRDLIAALKATGKPLVLVLMNGRPLALVKEDQQADA
                     ILETWFAGTEGGNAIADVLFGDYNPSGKLPISFPRSVGQIPVYYSHLNTGRPYNADKP
                     NKYTSRYFDEANGALYPFGYGLSYTTFTVSDVKLSAPTMKRDGKVTASVQVTNTGKRE
                     GATVVQMYLQDVTASMSRPVKQLKGFEKITLKPGETQTVSFPIDIEALKFWNQQMKYD
                     AEPGKFNVFIGTDSARVKKGEFELL"
     gene            45332..46249
                     /gene="osmF"
                     /locus_tag="OO357_001241"
     CDS             45332..46249
                     /gene="osmF"
                     /locus_tag="OO357_001241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter
                     substrate-binding protein OsmF"
                     /protein_id="HCR5886250.1"
                     /translation="MPLLKLWAGSLVMLAAVSLPLQAASPVKVGSKIDTEGALLGNII
                     LQVLESHGVPTVNKVQLGTTPVGRGAITSDELDIYPEYTGNGAFFFKDENDAAWKNAG
                     QGYEKVKKLDAEHNKLIWLTPAPANNTWTIAVRQDVAEKNKLTSLADLSRYLQEGGTF
                     KLAASAEFIERADALPTFEKAYGFKLGQDQLLSLAGGDTAVTIKAAAQQTSGVNAAMA
                     YGTDGPVAALGLQTLSDPQGVQPIYAPAPVVRESVLKEYPQMAQWLQPVFASLDAKTL
                     QQLNASIAVEGLDAKKVAADYLKQKGWAK"
     gene            46256..47413
                     /gene="yehY"
                     /locus_tag="OO357_001242"
     CDS             46256..47413
                     /gene="yehY"
                     /locus_tag="OO357_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehY"
                     /protein_id="HCR5886251.1"
                     /translation="MTYLRINPVLALLLLLTAIAAALPFISYAPNRLVSGEGLHLWQL
                     WPQTIWMLVGVGCAWLTACFVPAKKGSIFALILAQFVFVLLVWGAGKAATQLAQNGSA
                     LARTSLGRGFWLAAALALLACSDAIRRISTHPLWRWLLHMQIAIIPLWLLYSGTLNDL
                     SLMKEYANRQDVFDDALAQHLTLLFGAVLPALVIGVPLGIWCYFSTARQGAIFSLLNV
                     IQTVPSVALFGLLIAPLAALVTAFPWLGKLGIAGTGMTPALIALVLYALLPLVRGVVV
                     GLNQIPRDVLESARAMGMSGAQRFLHVQLPLALPVFLRSLRVVMVQTVGMAVIAALIG
                     AGGFGALVFQGLLSSAIDLVLLGVIPVIVLAVLTDALFDLLIALLKVKRND"
     gene            47406..48332
                     /gene="yehX"
                     /locus_tag="OO357_001243"
     CDS             47406..48332
                     /gene="yehX"
                     /locus_tag="OO357_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter ATP binding
                     protein YehX"
                     /protein_id="HCR5886252.1"
                     /translation="MIEFSHVSKLFGAQKAVNDLNLNFQEGSFSVLIGTSGSGKSTTL
                     KMINRLVEHDSGVIRFAGEEIRSLPVLELRRRMGYAIQSIGLFPHWSVAQNIATVPQL
                     QKWSRARIDDRIDELMALLGLESNLRERYPHQLSGGQQQRVGVARALAADPQVLLMDE
                     PFGALDPVTRGALQQEMTRIHRLLGRTIVLVTHDIDEALRLAEHLVLMDHGEVVQQGN
                     PLTMLTRPANDFVRQFFGRSELGVHLLSLRSVADYVRREERAEGEALAEEMTLRDALS
                     LFVARGCEVLPVVNTQGQPCGTLHFQDLLVEA"
     gene            48337..49068
                     /gene="yehW"
                     /locus_tag="OO357_001244"
     CDS             48337..49068
                     /gene="yehW"
                     /locus_tag="OO357_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine ABC transporter permease YehW"
                     /protein_id="HCR5886253.1"
                     /translation="MKMLRDPLFWLIALFVALIFWLPYSQPLFAALFPQLPRPVYQQE
                     SFAALALAHFWLVGISSLFAVIIGTGAGIAVTRPWGAEFRPLVETIAAVGQTFPPVAV
                     LAIAVPVIGFGLQPAIIALILYGVLPILQVTLAGLGAIDPSVTEVAKGMGMSRGQRLR
                     KVELPLAAPVILAGVRTSVIINIGTATIASTVGASTLGTPIIIGLSGFNTAYVIQGAL
                     LVALAAIIADRLFERLVQALSQHAK"
BASE COUNT        11859 a        12088 c        13187 g        11954 t
ORIGIN      
        1 tgaatctgag acaacatctg gcaaggctgg gacggaagtc actgtcgttc tcaaaatcgg
       61 tggagctgta tgacaaggtc atcgggcatt atctgaacat aaaacactat cagtaagttg
      121 gagtcattac ccaaaacaga gtggcgataa tcatcaccat cgcaaaccag aacacagcgt
      181 gataattcca gatctcggcg acgatgcccg ccactgatcc tgcgatgatc cagcccacgc
      241 gcgacgtgtt ggtatagagc gtggtggctg aacctgcctg accgggcatc aaatcctgaa
      301 aatagagcat cccgatgccg cccagaatgc caataaaaat agcatttagc agctgcaagc
      361 ccaacagaat ggcaggtgaa tgcgccatca gcattcctgc gtaaaaacag acgccaccca
      421 cggcagcaac gcgcattaag aaacgcttac ccagacgttt ggcgaaatat ccggcaatca
      481 acatcgtcgg gatttccagc ccggcggcgg tccccatcat cacaccggcc agtttctcgg
      541 gaagatgcag ttcgttgata ataaatagcg gcatgttgat gatgtacagg ctgttcgagc
      601 cccacatcaa tgtacaaatg acaaacagca gtagcgtatc gcgacggtta cggcgcggcg
      661 cttcaaccgt gccggtcgcc agcggaagct ctttttgcat cgacggtaaa aacagccaca
      721 ccatcacgcc gcaaacaata aacgctaccg ctgcgctcag atacattacc gtaaagctga
      781 aacccatcgc taacgcataa gccagcggtg ggccaatgac ccatgccagt gaaacctgag
      841 cgcgtaaaaa agagctgaac atcaccgcct cacgtccggt tttgtcggca tgttcacggg
      901 caagggcaaa catttgcggg ttagcggtcg agccaaagct gctaagaaag acgccaacga
      961 atagcaaaac aaagtagttg cgattccaag caaaaagggt gcaggccagc acgcctaaca
     1021 ggcagcaaaa gacaatcagc gatttgcgat cgccgcgctt atcagagcgc ccagcgagaa
     1081 actgacttac cagaatccca atgacagcgc tgccggtaaa gaagaatccc accatcgccg
     1141 gacgggcatg tacttcatcg gtaaggaaaa tactgagtgt cggggtttgc agagcacccg
     1201 caataccggt gagaaaggca acgattaaaa acgccgtcga ggtcaggtca aacgatttcg
     1261 cgctggagac tgcgggggag ttatgcatgc tggtcgatca ctggaagagg aagacgcgga
     1321 gtttacgctg cttgtcagaa aataaacaga cagcgaaatc aaaagcgcca cgaataatca
     1381 aaatgccatt tcactgttgc tgattggctg ctgacgctgc tgaaatgaaa ttgctgataa
     1441 cggattttcc catcagcaat taggaaaaat gacaaaaaat tgtgcagcac atcaaacttt
     1501 tgctcataac tttacggctt tccttgcgtg ctgaaacaga agtattatgc tttcttgaaa
     1561 cgtttcagcg cgatcttgtc tttaacccta agctaggttg gcgctttttt tctcatagag
     1621 gctgaatcgt ttcaattcag caagagagga gaactatgtt ccagttatcc gtacaggaca
     1681 tccatccggg cgaaaaggcc ggagacaaag aagaggcgat tcgccaggtc gctgcggcgc
     1741 tggtgcaggc cggtaatgta gcagaaggct acgtcaatgg catgctggcg cgcgaacagc
     1801 aaacctcaac gttcctcggc aatggtattg ctattccaca cggcactacc gacacccgcg
     1861 atcaggtgct gaaaaccggc gttcaggtat ttcagttccc ggaaggcgtc acctggggtg
     1921 acggtcaggt agcgtacgtg gcaatcggta ttgctgccag ctcggataaa catctgggcc
     1981 tgctacgcca gctgacccac gtactgagcg atgattccgt tgctgaacaa ctgaagtcag
     2041 caacaacagc agaagaactt cgcgcattac tgatgggcga aaagcagagt gagcagctga
     2101 agctcgacaa cgaaatgctg acgctggata tcgtcgccag cgatctgctg actcttcagg
     2161 cgctgaacgc tgcgcgtctg aaagaggcga gggcagttga cgccactttc gtcaccaaag
     2221 ccatcaatga acaaccgctg aatctcggac agggtatctg gctgagcgat agcgccgaag
     2281 gcaatctgcg tagtgcgatt gcggtaagcc gtgcggcaaa tgcttttgat gtggacggcg
     2341 aaacggcagc catgctggtg agtgtggcga tgaatgacga tcagcccatc gcggttctta
     2401 agcgtctcgc tgatttgttg ctcgacaata aagctgaccg cttgctgaaa gcggatgcgg
     2461 caacgttgct ggcgctgctg accagcgatg atgcgccgac cgacgacgtg ttaagcgcgg
     2521 agtttgtggt gcgcaatgaa cacggcctgc atgctcgtcc aggtaccatg ctggtcaata
     2581 ccattaaaca atttaacagt gatattaccg tgacaaacct tgatggcacc ggcaaaccgg
     2641 caaacggacg tagtctgatg aaagttgtgg cacttggcgt taagaaaggt catcgcctac
     2701 gctttaccgc ccagggtgca gatgctgaac aggcgctgaa agccattggc gacgctatcg
     2761 ctgctggtct tggggagggc gcataatgag cagacgtgtt gctacgatca cccttaatcc
     2821 ggcttatgac cttgttggtt tctgcccgga aattgaacgc ggcgaagtga acctggtgaa
     2881 aaccaccggt ctgcatgcgg cgggtaaagg catcaacgtg gccaaagtat taaaagacct
     2941 gggaattgat gtcaccgttg gcggcttcct gggtaaagac aatcaggatg gttttcagca
     3001 actgttcagc gagctgggca ttgccaaccg tttccaggtt gtacaggggc gcacccgaat
     3061 taacgttaag ctgacggaaa aagacggcga agtgaccgac ttcaacttct cgggtttcga
     3121 agtcaccccc gccgactggg aacgctttgt gactgattct ctgagctggc tcggtcagtt
     3181 cgatatggtc tgtgtcagcg gaagcttacc gtcaggcgtc agcccggaag cgttcaccga
     3241 ctggatgact cgcctgcgta gtcagtgtcc ttgcattatc tttgatagta gccgtgaagc
     3301 gttagtagca ggtttgaaag cggcaccgtg gctggtgaaa cctaaccgcc gcgagttgga
     3361 aatctgggca ggccgtaaac tgcctgaaat gaaagatgtg attgaagctg cacatgcgct
     3421 acgtgaacaa ggtattgcgc atgttgttat ttcactgggt gccgaaggcg cgctttgggt
     3481 taatgcctcc ggcgaatgga tcgctaaacc accgtcagtc gatgtcgtaa gcaccgttgg
     3541 cgcaggggat tctatggttg gtggcctgat ttatggcttg ctgatgcgtg aatccagtga
     3601 acacacactg cgtctggcga cagctgttgc agccctggcg gtaagtcaaa gcaatgtggg
     3661 tattaccgat cgtccgcagt tggccgcaat gatggcgcgc gtcgacttac aaccttttaa
     3721 ctgacagcag gagaggcata atgaaaacgc tgctgattat tgacgctaat ctcggtcagg
     3781 cacgcgccta tatggcgaag accctgctgg gcgcggcggc gcgaaaagca aaactggaaa
     3841 tcatcgacaa tccgaacgac gcagaaatgg cgattgttct cggtgattcc atcccgaatg
     3901 acagcgcgct gaacggtaaa aatgtctggc tgggtgatat ttcccgggca gttgcgcacc
     3961 ctgagctgtt cctgagtgaa gccaaaggcc atgcgaaacc ttacactgcg ccggtcactg
     4021 cgacagcacc ggttgccgcc agcggtccga aacgcgtagt tgcggtgact gcttgcccga
     4081 ctggcgtagc acacaccttt atggcggctg aagccattga aaccgaagcg aaaaaacgtg
     4141 gctggtgggt gaaagttgaa acccgtggtt ctgttggcgc gggtaatgca atcactcccg
     4201 aggaagtagc cgcagcggat ctggtgattg tggcggcaga tatcgaagtg gatctggcga
     4261 aatttgctgg taaaccgatg tatcgtacct ctaccggtct ggcgctgaag aaaacctcgc
     4321 aggaactgga taaagcggtt gctgaagcaa cgccgtatga accggcgggc aaagctcaaa
     4381 cggcgaccac tgaaggtaag aaagagagtg caggcgctta tcgtcacttg ctaacgggcg
     4441 tctcttacat gctgccgatg gtcgttgctg gtggtctgtg tatcgcgctt tcttttgctt
     4501 ttggtatcga agcgtttaaa gagccgggta cgttggcagc ggcgctgatg cagattggtg
     4561 gtggttcagc ctttgcgctg atggtgccgg tactggcagg ttatattgcc ttctccattg
     4621 ccgatcgtcc gggcctcacg ccgggtctga ttggcggtat gctggcggtc agcaccggtt
     4681 ctggcttcat tggcggtatt attgcgggct tcctggctgg ttacattgcg aagttaatca
     4741 gtacgcaact gaaactgccg cagagtatgg aggcgctgaa accgatcctg atcattccgc
     4801 taatttccag tctggtggtc ggtctggcga tgatctacct gatcggtaaa ccagttgctg
     4861 gcattctcga agggctgact cactggctgc agaccatggg gactgcgaat gcggttctgc
     4921 tgggggcgat cctcggtggc atgatgtgta ctgacatggg cggtccggta aacaaagcag
     4981 cgtacgcatt cggtgtgggt ctgctgagta ctcaaaccta tggcccgatg gcggcgatta
     5041 tggcggcagg tatggtgcca ccgctggcaa tgggcctggc aacaatggtg gcgcgtcgca
     5101 aattcgacaa agcgcagcag gaaggtggca aagccgctct ggtattggga ctgtgcttca
     5161 tttcggaagg tgcaattccg tttgctgctc gtgatccgat gcgtgtgctg ccgtgctgta
     5221 tcgtgggtgg ggcgctgact ggcgcaatct caatggcgat tggtgcgaaa ctgatggcac
     5281 cgcacggtgg tctgtttgtt ctgctgatcc ctggcgctat tacgccggta ttgggttacc
     5341 tggtagcaat tattgccggt acgctggtgg cgggtttggc ctatgccttc ctgaaacgtc
     5401 cggaagtgga cgcagtagcg aaagcagcgt aataaaaggt gtgttacagg gcagaaattt
     5461 cctgccctgt aacatttgat gagcgcaaag agaatgacca gcttgtgaat gtaattttat
     5521 gaatttaaat ggttttatct ttcagtagaa acttcaaacg ttcatcaaac actaaggtta
     5581 tttcctcaag ggcagattat tttctgccct tttttagtgt tcctgttctg tgcatcccgt
     5641 cacaaattcc tcgcttttgt aattcaggca tttaatcgca ttttgtgagc ctgctcaaat
     5701 ctgcccattt tgcctctctg gcggtaattt actttacgta aaacatgcat actatgagca
     5761 catgtttaaa tatgaacaca tgtttaaacc gtctttgcag tatctatatt cttgtggatt
     5821 gacagggttg gtaatttttt accagaggac gtgctatgcg cgaaaaggat tatgtcgtaa
     5881 ttataggttc ggcgaatatt gatgtcgccg gatattcaca tgaatcatta aattatgcgg
     5941 attcaaatcc aggtaaaata aaatttacgc ctggtggagt agggcgcaat attgcacaaa
     6001 acctggcgtt gctgggtaac aaagcctggc tactgagcgc cgtaggcagt gatttttatg
     6061 gtcaatcgct gctaacgcaa accaatcaat ctggtgttta tgtcgataaa tgcctgattg
     6121 tgccgggaga aaatacgtcg agttatttat cattactcga taataccggt gaaatgctgg
     6181 ttgctataaa tgacatgaat attagcaacg ctattacagc tgaatatctc gcacagcacc
     6241 gtgaatttat tcagagggca aaggtcattg tcgcggactg taatatcagt gaagaggcac
     6301 tggcatggat tctggataat gccgccaacg tacccgtatt tgtcgatccg gtttcagcgt
     6361 ggaaatgtgt caaagtgcgc gaccgtctaa atcagaacca cactctgaag ccaaaccgcc
     6421 ttgaagcgga aaccctgagt gggattgcgc tgtcagggcg tgacgatgtg gcaaaagttg
     6481 ctgcctggtt ccatcaacat ggcctgaacc gactggtatt gagcatgggc ggcgacggcg
     6541 tttattacag cgatatcagc ggtgaaaatg gctggtctgc gccgatcaaa accaatgtta
     6601 ttaatgttac cggagcgggc gatgccatga tggcgggact tgcttcgtgt tgggtagacg
     6661 gaatgccgtt tgccgaatct gttcgtttcg cacagggatg ttcgtcaatg gcgctctcct
     6721 gtgaatacac caataacccc gatttatcga ttgccaacgt tatatcgtta gtggagaacg
     6781 cagaatgtct gaattaaaaa tttcccctga attattacaa atttctccgg aagtgcagga
     6841 cgctttaaaa aacaaaaaac cggttgtggc gctggaatcg accattattt ctcacgggat
     6901 gccgttccca caaaatgccc agaccgcaat tgaagtagaa gaaactattc gtaaacaggg
     6961 cgcagtacct gccactatcg ccattattgg cggcgtgatg aaagtgggtt taagcaaaga
     7021 aaaaattgaa ttactgggtc gtgaagggca taacgtgacc aaagttagtc gtcgcgattt
     7081 accttttgtt gttgccgccg gaaaaaatgg cgcaaccact gtggcttcaa cgatgattat
     7141 tgcggcgctt gccggaatta aagtatttgc caccggggga attggtggtg tgcatcgcgg
     7201 ggcggaacat accttcgata tttctgccga tttgcaagaa ctggcaaata ctaatgtcac
     7261 cgttgtttgt gccggggcga aatctattct cgatttagga ttaaccactg agtatttaga
     7321 aaccttcggt gtgccgttaa ttggctatca gactaaagcg ctgcctgcgt ttttctgccg
     7381 taccagctcg tttgacgtca gcattcgtct cgacagcgcc agtgaaattg cccgtgcaat
     7441 ggcggtgaaa tggcaaagcg ggctgaacgg tggcctcgtg gtagcgaacc cgatcccgga
     7501 acagtttgcg atgccggagg aatctatcaa tgcagccata gatcaagccg tcgccgaagc
     7561 cgaagagcag ggcgttattg gtaaagaaag tacaccgttc ctgctggcgc gcgttgctga
     7621 gctgaccagc ggtgacagcc tgaaatccaa tatccagctg gtgttcaaca acgccattct
     7681 ggcgagtgaa attgccaaag aataccagcg tctcgcgggt taatttctga cgggcagaaa
     7741 gccgtcgccc gtactcatca agcataccaa aacctggcgc tgacgccggg tttactggca
     7801 tgaagggaaa atcattatgg atataatgag aagtgttgtg gggatggtgg tgttactggc
     7861 aatagcattt ctgttgtcag tgaataaaaa gagcatcagt ttgcgcacgg ttggagccgc
     7921 actgctgctg caaatcgcta ttggtggcat catgctctac ttcccaccgg gaaaatgggc
     7981 agtagaacag gcggcattag gcgttcataa agtgatgtct tacagtgatg ccggtagcgc
     8041 cttcattttt ggttcgctgg ttgggccgaa aatggatgtc ctgtttgacg gtgcgggttt
     8101 tatcttcgcc tttcgcgtac ttccggcgat tattttcgtt actgcgctca tcagtctgct
     8161 gtactacatt ggcgtgatgg ggctgctgat tcgcatcctt ggcagcattt tccagaaagc
     8221 cctcaacatc agcaaaatcg aatcttttgt tgcggttacc actattttcc tcgggcaaaa
     8281 tgagatcccg gcgatcgtta aaccgtttat cgatcgcgtg aatcgcaacg agttgtttac
     8341 cgcaatttgt agcgggatgg cgtccattgc tggttcggtg atgattggtt atgccggaat
     8401 gggcgtacca attgactacc tgttagcggc atcgctgatg gcgatccccg gcggtatttt
     8461 gtttgcacgt attcttagcc cggcaaccga gccttcgcag gtcacatttg aaaatctgtc
     8521 gttcagcgaa acgccgccaa aaagctttat cgaagcggcg gcgagcggtg cgatgaccgg
     8581 gctgaaaatc gccgctggtg tagcgacggt ggtaatggcg tttgtcgcaa ttattgcgct
     8641 gatcaacggc attatcggcg gaattggcgg ctggtttggt ttcgccaatg cctctctgga
     8701 aagtattttt ggctatgtgc tggcaccgct ggcgtggatc atgggtgtgg actggagtga
     8761 tgccaacctt gctggtagcc tgattgggca gaaactggcg attaacgaat tcgtcgctta
     8821 cctgagtttc tccccatacc tgcaaacggg cggcacgctg gaagtgaaaa ccgtggcgat
     8881 tatatccttt gcgctttgtg gttttgctaa ctttggttct atcggtgttg tcgttggcgc
     8941 attttcggct atttcgccaa aacgtgctcc agaaatcgcc cagcttggtt tacgggcgct
     9001 ggcagcagca acgctttcca acctgatgag tgcgactatt gccgggttct ttattggtct
     9061 ggcgtaatgc tcgatttgta gaccggataa aacgtattaa gcgttgcatc cggcaatcgc
     9121 gctattgcaa ataattttta ctgcatcatc ccggagaatt tattctccgg gtccatctcc
     9181 agcgccagtc ctgacaactg ctttctgttt ttaattagat atcctttctt ttaattaata
     9241 aaccgtcgtg aatgaattgc gcgagaacat ataaaagatg tcgataagaa acgcctaaat
     9301 actctgccgc ttgcgtatgc ttttcgtgat aaagatcacc ttcttgtgag agtaatataa
     9361 atgctgccag gcgattcact aacggaaatg attgattctg agttaaagaa acaatattac
     9421 gataattttt atgacttaag gtgacgcaaa gttttcgtaa aaatagggtg tcgtttaata
     9481 acagcggacg gtaatgtttc atagggagcg caaggcacca acactcttca atagcctgca
     9541 ccgcacgcgg ttcatggtct ttgtcgatta actcaatctc gccaataaaa cagggagcgg
     9601 caaagaaatc gatcagcgac acgcgaccat tggcaagcgt ggcgtaaagc ctggcgcggc
     9661 ctcgcgtcag ataaaacagc catgacggct gttgcccttc ctgcacaatg tagtcacgcg
     9721 ctaaaaaatg aaacagccgc gtatcggctg aaacatccgt tgaaaagcaa tccttaaacg
     9781 cagattcact catcaattgc tgcttaaggt cattattgtg gatttctttc acgatgttta
     9841 tccgctatga gatttctcat atttagcata cgtacgctgt tgttaagatt caatcattgg
     9901 cccggtcgaa ttttctttgc tgtagaaaaa aataacgcaa ctggaaacag aggaaataaa
     9961 caatggaaaa gagaaaaatt attctggatt gtgagccggg tcatgatgat gctattgcaa
    10021 tgatgatggc agcgaaacat ccggcaatag atttattagg catcactatt gtagcgggta
    10081 atcagacgct tgataaaaca ttaattaatg gcctgaatgt ttgccagaaa ctggagatta
    10141 atgttccggt ttatgcgggg atgccgcaac ccattatgcg taaacaaatc gttgccgata
    10201 atattcacgg tgaaaccgga ctggatggcc cggtattcga gccgctgacc cgccaggcag
    10261 aaagtaccca tgcggtgaaa tatatcatcg ataccctgat ggcaagcgat ggcgatatca
    10321 ctctggtgcc ggttggtccg ctttcaaata tcgcggtggc aatgcgtatg caacccgcaa
    10381 tcctgcccaa aatccgtgaa attgtgctaa tgggcggcgc ttacggtaca ggcaacttca
    10441 ccccatctgc cgagttcaat atctttgccg acccggaagc cgcccgcgtg gtgttcacct
    10501 ccggcgttcc attagtgatg atgggcctcg atcttaccaa ccagaccgtt tgcaccccgg
    10561 atgtaattgc tcggatggaa agggtaggcg gccctgccgg agagctgttc agcgacatca
    10621 tgaacttcac tctcaaaacg cagttcgaat actacggcct ggctggcggc ccggtgcatg
    10681 acgccacctg cattggttat ctgatcaacc ctgatggcat taaaacccag gatatgtacg
    10741 tcgaagtgga cgtcaacagc ggcccttgct acgggcgtac ggtctgcgac gaactgggcg
    10801 ttcttggcaa gcccgctaat accaaagtcg gcatcactat tgataccgac tggttctggg
    10861 gcttagtcga agagtgcgtg cgcggctaca tcaaaaccca ttaagtgttg ataacaagcc
    10921 gggacagtat cgcaagatgc aggcccggct atttctgatg ggcagaaagc cgtcgcccaa
    10981 aatgacatga agagataatc actatggatg tcatgagaag tgttctggga atggtggtac
    11041 tgctggcaat tgcgttttta ctgtcagtaa acaagaagaa gatcagcctg cgtaccgttg
    11101 gcgcggcgtt agtgttacag gtcgtgattg gcgggattat gctttggtta ccgccagggc
    11161 gttgggtcgc agaaaaagtc gcttttggcg tccacaaagt gatggcgtac agcgacgcag
    11221 gtagcgcatt tatcttcggt tctctggtcg gaccgaaaat ggatacctta tttgatggcg
    11281 caggatttat ctttggtttc agggtattac cggcaattat cttcgtcact gcactggtga
    11341 gtattctcta ctacatcggt gtgatgggga tcttaattcg cattctcggc ggtatcttcc
    11401 agaaagcttt aaatatcagc aagatcgagt cattcgtcgc ggtcaccacc attttcctcg
    11461 ggcaaaacga aattccggcg atcgtcaaac cctttatcga tcgtctgaat cgcaatgaat
    11521 tatttacagc gatttgtagt ggcatggcct cgattgctgg ttcgacaatg attggttacg
    11581 ccgcactggg cgtacctgtg gaatatttgc tggcggcatc gttaatggcg atccctggcg
    11641 ggatcttgtt tgcccgcctg ttaagcccgg ctacggaatc ttcgcaggtt tcttttaata
    11701 acctctcttt caccgaaaca ccgccaaaaa gcattattga agccgatgcg acaggggcaa
    11761 tgaccgggct gaaaatcgcc gccggtgtag cgacagttgt tatggcattt gtcgccatca
    11821 ttgcgttaat taacggtatt atcggcggcg ttggcggctg gtttggtttt gaacatgcct
    11881 cgctggagtc cattttaggt tacctgctgg ccccacttgc gtgggtaatg ggtgttgact
    11941 ggagtgatgc aaatcttgcc gggagtttga ttggacagaa actggcaata aatgaatttg
    12001 tcgcttatct caatttctca ccctatctgc aaacggctgg cactctggat gctaaaaccg
    12061 tggcgattat ttccttcgcg ttgtgcggtt tcgctaactt tggttctatc ggggtggtgg
    12121 tgggggcgtt ttctgcggtt gcgccacacc gtgcgccgga aatcgcccag cttggtttac
    12181 gcgcgctggc ggcggcgaca ctttctaatc tgatgagtgc caccattgcc gggttcttta
    12241 ttggtttagc gtagtgtgca atttatgagc cggattagat gcggtcaagc gtcgaatccg
    12301 gcaacattgt tacaccattg gcactaatga cagcgcgtta tcggcagaca gggtagggtt
    12361 gtttaagctg ccgctggcgc gtgaaattgc cgcgcaggcc atcgcaaaac gggcgctgtc
    12421 acggaaattg tttccctcca gaaagctata taccagaccc gccataaaac catcgtcagc
    12481 accaaaactg tctaccgtcg tatgcgctgg cacagtcagc aaaaattgtt caccatcctt
    12541 ttcgctgcaa taaactgact catcgggcaa ataaacaaac agttgctgaa caccttgctg
    12601 atgcaatgca ttcactgcgg cattacggtc agcatcgctg gtgatcgcct gtccccataa
    12661 aatttccagc tccggtaaag tgggtttcag ggtgtgaata tgcgccagcc agtgtttgat
    12721 tttgcccgct ttgaattctg aaacggtatc gacaaacacc gggatctcat cagcgagggt
    12781 aaagacccat tccagcgcct cggctgtcag gttgcaatca gccagtacca cgcccgcatg
    12841 acgaagtaaa tcgcgcgacc cgttcagtag ttgcggtgtc aactgttcca gcagatgggt
    12901 atcgttaatt gccagcacgg tttgatcgtc tcgattggca attgccagat acgtcgatgt
    12961 gctttgacca tgcaaacgaa cgcagccgga gacattcacg ccggcacggc gcgtttcttc
    13021 caggagcatt tcgccataaa agtcatcgcc aatcactgaa agcaaatgga cgtcacggcc
    13081 taacagcgcc agattgtggg cgatattgcg tccaacgccg cctgccgaac aatgaattgt
    13141 accgggatga gaagccgctt gcgggtaatg gatatccgcc atcccgcgaa tatccatatt
    13201 gattgtcccc accactacgc agtattcctg ctcggtgaga atgtaacctt tgtctttaat
    13261 ccggcctttg cgcattaaat ccataatatg cgccgcaacg cgcgaacgac tgatttgcag
    13321 catgtccgca atttcgttct gctgaatcag cgggttacgc cgtaaaattg caaggatctc
    13381 cttttcccgg ttatttatct tcaggctacc gctttttcag tttgttgcgc tttcagccag
    13441 gcgatctctt ctgcccagat atccgggttg atggtttcaa ggatcagcgg aatgccgtcg
    13501 aaacggtcgt tctgcataat ccagcggaac gcatcatgac cgatattgcc ttcaccgagg
    13561 ctatgatggc ggtcaacgcg gctgccaaag gtacttttcg catcgttaag gtgcatcccg
    13621 cgcaaatact taaagccgac aatacgggca aaatcagcga acgttttttc gcattcggct
    13681 ggagtacgca aatcataccc ggcagcgaaa gcatggcagg tatcaatgca gacgccgacg
    13741 cgggatttat cttccacgcc gtcgataatc gccgcgagat gttcgaattt aaaccctaag
    13801 ttactgccct gaccggcggt gttttcaatc accgctgtta caccttgagt tttatccagc
    13861 gcaatgttga tggattcggc aatacgcgca aggcaatcct cttctgaaat ctgcatcaga
    13921 tggctgccag ggtggaagtt gagcaaagaa agccccagtt gttcgcaacg ctgcatttca
    13981 tctataaagg catcgcgcga tttttccaga gcttcagtga ccggatgtcc gaggttgatc
    14041 agataactgt cgtggggaag aatttgcgcc gatgtgtagt gatatttttc acaggcggct
    14101 ttgaattcat cgatggtttg cgtcgtgagc ggtgcggcac gccactgacg ttggtttttg
    14161 gtgaacaagg caaacgcggt tgcgtcgatt tcggcggcgc gaattgcggc atttgccaga
    14221 ccgccagcag cactaacgtg cgctccaatg tatttcatgc gaggactcct gttaaacccg
    14281 ctggaggaaa acggtaatga tagcgggtta acaggagcaa gaagtagtgg tttatgcgat
    14341 gacgctttga atcacatagt taatcgcacc accaccaaca atcagccagg caaacagtac
    14401 cagtgccatc agcagaggtt tcgccccagc ttttttcagc gcgctgacgt gagtggtcag
    14461 acccagcgcc gccatcgcca ttgccagcag gaaggtatcc agcgttacca gcatgttcac
    14521 cacgctctgc ggtaacaggt ggaacgagtt aaagatggca actacgatga acaagatggc
    14581 aaaccacgga atagtgattt tgcttttctc gccgctgttc gccccagaca gctgtttaac
    14641 acgcgccgcc agcaggatga ggaacggagc cagcatcatc acgcgcagca ttttggaaat
    14701 aactgctgcg ttttctgcat ccgggctgat ggcatgacct gccgccacca cctgcgccac
    14761 ttcgtgcaca gtagaaccaa tgtagatacc gaaagtttcc ggactaaacc attgagacat
    14821 cagcggatat atcgccgggt agaggaaaat cgcgacggtc ccgaagataa caacggttgc
    14881 aacagccacg gttactttac tggcttccgc tttcactacc ggctcagtcg ccagtaccgc
    14941 ggcagcacca cagatactgc taccggcacc gatcaaccaa ctggtgtgct tatccagacc
    15001 aaacactttc tgccccagga agcaagccag caggaaggta ctggacagcg tcagcacgtc
    15061 aatgatgatc ccactgatac cgacatcggc aatttgcgag aacgtcagac ggaagccata
    15121 aagaatgata cccagacgta ataaatattg cttggcaaac agcacaccac cgtcacagct
    15181 tttccagatg tgcggataga tggtgttgcc taaaaccatc cccaacaaga ttgcgagggt
    15241 gagggcacta aacccggcac ccgcaaccgc gggaatggaa ccaccccaca gggcgacccc
    15301 ggtgataact gcactcaggg ctaaccccgg aataaaatgc cacagtgtac gatgttgttt
    15361 ctgtaaggtg atattcgtca taaccctctc ctttaaccgg gcataaggtt acgactaact
    15421 ggtttaaaaa taaaattgat tatatattta taattaatct ttataagtgg taagcgacta
    15481 tgcacatcac cctccggcag ttggaagttt ttgcagaagt attgaaaagt ggatcaacca
    15541 cccaggcgtc ggtgatgctg gcgttgtcgc aatcagcagt gagcgcagcc ttgactgacc
    15601 tggaagggca gcttggcgtt caactgtttg atcgcgtggg gaaaagactg gttgttaatg
    15661 aacacgggcg gctgctctat ccgcgagcgt tggcactgct tgaacaggcg gttgaaatcg
    15721 aacaactgtt tcgcgaagac aacggcgcga ttcgtatcta tgccagtagt accatcggta
    15781 actacattct gcctgcagtt atcgcccgtt atcgccatga ttatccgcag ttgccgattg
    15841 aacttagcgt tgggaacagc caggacgtga tgcaagcggt gctggatttc cgcgttgata
    15901 ttggctttat tgaaggaccg tgccacagca ctgaaatcat ttctgaaccg tggctggaag
    15961 acgagctggt ggttttcgcc gcgccgactt cgccgttggc tcgtggtccg gtcaccttag
    16021 aacagctggc cgctgcgccg tggatcctgc gtgaacgcgg ttccggcacg cgggatattg
    16081 tcgattatct gttgctgtca catttaccga agtttgagat ggcgatggaa ttaggtaact
    16141 ccgaggcaat caaacatgcg gtgcgtcatg ggttgggaat tagttgcctg tcgcgacgtg
    16201 tgattgaaga tcaattgcag gcaggcacat taagtgaagt tgcggtccct ctgccgcgcc
    16261 tgatgcgtac gttgtggcgt atacatcatc ggcaaaaaca cctttccaac gcgctacggc
    16321 gctttctgga ctattgcgat cccgcaaatg tgccgcgtta agttgctgta caagaacatg
    16381 ctggtgctgt gtcgatttcg tgacgcagcg ccttcagcat gcattcgcca gaaaagagat
    16441 tggctgcttt acttataatc cctgggcgat catgaaggtg tcttataacc gtgtatttct
    16501 gccggaagga ttgccaatcg tctgctacaa tcgcgccatc atttttaaga tggatagcat
    16561 ttttgtatgg gttccgaaac taaaactaca gaagcgccgg gcttacgccg tgaattaaag
    16621 gcgcgtcacc tgacgatgat tgccattggc ggttccatcg gtacaggtct ttttgttgcc
    16681 tctggcgcaa cgatttctca ggcaggtccg ggcggggcat tgctctcgta tatgctgatt
    16741 ggcctgatgg tttacttcct gatgaccagt ctcggtgaat tggctgcata tatgccggtt
    16801 tccggttcgt ttgccactta cggtcagaac tatgttgaag aaggctttgg cttcgcgctg
    16861 ggctggaact actggtacaa ctgggcggtg actattgccg ttgacctggt tgcagcacag
    16921 ctggtcatga gctggtggtt cccggataca ccgggctgga tctggagtgc gttgttcctc
    16981 ggcgttatct tcctgttgaa ctacatctct gttcgcggct ttggtgaagc ggaatactgg
    17041 ttctcactga tcaaagtcac gacagttatt gtctttatca tcgttggtgt gctgatgatt
    17101 atcggtatct tcaaaggcgc gcagcctgcg ggctggagca actggacaat cggcgaagcg
    17161 ccgtttgctg gtggttttgc ggcgatgatc ggcgtagcta tgattgtcgg cttctctttc
    17221 cagggaaccg agctgatcgg tattgctgca ggcgagtccg aagatccggc gaaaaacatt
    17281 ccacgcgcgg tacgtcaggt attctggcga atcctgttgt tctatgtgtt cgcgatcctg
    17341 attatcagcc tgattattcc gtataccgat ccgagcctgc tgcgtaacga tgttaaagac
    17401 atcagcgtca gtccgttcac cctggtgttc cagcacgcgg gtctgctctc tgcggcggcg
    17461 gtgatgaacg cagttattct gacggcggtg ctgtcagcag gtaactccgg tatgtatgcg
    17521 tctactcgta tgctgtacac cctggcgtgt gacggtaaag cgccgcgcat tttcgctaaa
    17581 ttgtcgcgtg gtggcgtgcc gcgtaatgcg ctgtatgcga cgacggtgat tgccggtttg
    17641 tgcttcctga cctccatgtt tggcaaccag acggtatacc tgtggctgct gaacacctcc
    17701 gggatgacgg gttttatcgc ctggctgggg attgccatta gccactatcg cttccgtcgc
    17761 ggttacgtat tgcagggaca cgacattaac gatctgccgt accgttcagg tttcttccca
    17821 ctggggccga tcttcgcatt cattctgtgt ctgattatca ctttgggcca gaactacgaa
    17881 gcgttcctga aagataccat tgactggggc ggcgtagcgg caacgtatat tggtatcccg
    17941 ctgttcctga ttatttggtt cggctacaag ctgattaaag gaactcactt cgtacgctac
    18001 agcgaaatga agttcccgca gaacgataag aaataagttt cctcccttcc ttgctaagcc
    18061 ctctcaaccg agagggcttt ttcaattcca tttccctgac aaatcatgcg gatataaaat
    18121 ttaacatttg gattgataat tgttatcgtt tgcattatcg ttacgacgca atcaaaaaag
    18181 gctgacaaat cagaggctgt tccggctttc tgggatgatc acctgcatac aaataagtcc
    18241 accgcgatgc tgccgtacgc aaggggacgt aaagaagatg tgagcgatac ccattttatt
    18301 ttcgtagtta cctcatggag atatggaatg tttaggttga accctttcgt acgggtcggg
    18361 ctgtgtttgt ccgctatttc ttgtgcatgg cctgtgttag cggtcgatga tgatggcgaa
    18421 acgatggttg tcactgcatc ttccgtggaa caaaatctta aagatgcacc cgccagtatc
    18481 agcgtcatta cccaggaaga cctgcagcga aaaccggtac agaatctgaa ggatgtcctc
    18541 aaagaagtgc ctggcgtaca actgacgaac gaaggggata accgtaaggg cgttagtatt
    18601 cgtggtctgg acagcagcta caccctgatt cttgtcgacg gtaaacgcgt taactcccgc
    18661 aatgccgtct tccgccacaa tgatttcgat ctgaactgga tcccggtcga ttccatcgaa
    18721 cgtattgaag tggttcgtgg cccgatgtcg tcgctatacg gctccgatgc gctcggcggt
    18781 gtagtgaata tcatcaccaa aaaaatcggt cagaaatggt cgggtaccgt taccgtcgat
    18841 accaccattc aggaacatcg cgatcgcggt gatacctata acggtcagtt ctttaccagt
    18901 ggaccattaa ttgatggtgt gctgggaatg aaagcttacg gcagcctggc aaaacgtgaa
    18961 aaggatgacc cgcaaaactc aacgaccacc gataccggag aaacgccgcg tattgaagga
    19021 ttctccagcc gcgacggcaa tgtcgaattt gcctagacac cgaatcaaaa tcacgatttt
    19081 actgccggat acggtttcga ccgtcaggat cgtgattccg attcgctgga caaaaaccgc
    19141 ctggaacgcc agaactactg cgtcagccat aatgggcgtt gggattacgg caccagcgaa
    19201 ctgaaatcct acggtgagaa agtcgagaac aaaaaccctg gcaacagcag cccgataact
    19261 tccgaaagca atacggtcga cggcaaatac acgttgccgc tgacggcgat taatcagttt
    19321 ctcacggttg gcggtgaatg gcgtcacgac aaacttagcg atgcggtgaa cctgaccggg
    19381 ggaaccagct ccaaaacgtc tgccagccag tacgcgctgt ttgtggaaga tgaatggcgg
    19441 atcttcgagc cgctggcgct gacgaccggc gtgcgtatgg acgatcacga aacctacggt
    19501 gaacactgga gtccgcgtgc ctacatggtt tataacgcca ccgacaccgt aacggtgaaa
    19561 gggggctggg cgacggcatt taaagcacct tctctgttgc aacttagccc tgactggacg
    19621 agcaattcct gccgtggcgc atgtaagatt gtgggtagcc cggatctgaa accagaaacc
    19681 agcgaaagtt gggagctggg gctttactac atgggtgaag aaggctggct ggaagggggt
    19741 gaatccagcg ttatcgtttt ccgtaacgat gtgaaagatc gtatcagcat cagccgtacg
    19801 tctgacgtca acgctgcacc gggctaccaa aactttgttg gttttgagac gggcgctaac
    19861 ggacggcgca taccggtatt tagctactac agcgttaaca aagctcgtat tcagggcgtg
    19921 gaaaccgaac tgaaaattcc gttcaacgat gaatggaaac tgtcgatcaa ctacacctac
    19981 aacgatggtc gtgatgtcag caacggcgaa aacaaaccgc tatccgatct gccgttccat
    20041 actgctaacg gtacgctgga ctggaaaccg ctggcgctgg aagactggtc attctatgtt
    20101 tctggtcact ataccgggca gaaacgcgcc gacagcgcga cggctaaaac accgggcggt
    20161 tataccatct ggaataccgg cgcggcctgg caggtgacta aagacgtcaa actgcgcgca
    20221 ggcgtgctga accttggcga caaggatctc agtcgtgacg actacagcta taacgaagac
    20281 ggacgtcgtt actttatggc agtggattat cgcttctgat gagaagatgc ccggcgaacc
    20341 gggcggactt tcacttcagt aaatactgcg catggaagcg caggtgatcc tctataaaag
    20401 aggcgatgaa gtagtaactg tgatcatatc ccggctgaat acgcagcgtc atcggccacg
    20461 ctttctggcg cgcggcttct gccaataccg caggttgcaa ctggtcggca agaaactgat
    20521 cattatcgcc ctgatcgata agcgtcggga tcgcatcctg cgcgttactg gcatacatca
    20581 gcgcgcaact atcccattcc agcatgcatt tttgtcctca cctaaatagg tgctaaacgc
    20641 tttgattccc cacgggacgc tgcacggatt cacaatgggc gcaaaggccg aaacgctggt
    20701 gtatttaccc ggatttttca gcgccataat cagcgcaccg tgaccaccca ttgagtgacc
    20761 gctaatggcg cagcggtcgc tgacattaaa ttgcgactga accagcgccg gtaattcatc
    20821 gcgcagataa tcatacatcc ggtaatgcgt cgcccacggc ggttgcgtgg cattaagata
    20881 aaagcctgcg ccctggccta aatcgtagcc atcgtcgttg gcaacctttt cgccgcgcgg
    20941 gctggtgtct ggcatcacca gtacaatccc cagttccgcc gctacccgct gggcacccgc
    21001 cttggtggtg aagttctcgt cattgcaggt taatccggaa agccagtaca gcactggtgg
    21061 cggggtgtga tcacgagggg gggggagaaa gatactgaac gtcatcgggc agtttaaggt
    21121 actggagtcg tgtcgccagc gttgctgcca gccttcaaaa cagcggtgct cttcgagcat
    21181 ttccatgcgt ggctccttgt tgtgttgttt gcaatttctt catcataccg tttattcaat
    21241 gtgctgtgag taactttcac ttccgtattt gcataacgat gttttaacat ctgctgatga
    21301 aaggcagcgg caattacaat aattatcgct gtgaatactg gattatgtgc gccgcctcac
    21361 gcacaataat caagctgtaa atcagcttaa taactttgcc cccacgcagg gtggaggcgt
    21421 cacacctgca ggagaaatca taaatgccat cactcagtaa agaagcggcc ctggttcatg
    21481 aagcgttagt tgcgagagga ctggaaacac cgctgcgccc gcccgtgcat gaaatggata
    21541 acgaaacgcg caaaagcctt attgctggtc atatgaccga aatcatgcag ctgctgaatc
    21601 tcgacctggc tgatgacagt ttgatggaaa cgccgcatcg catcgctaaa atgtatgtcg
    21661 atgaaatttt ctccggtctg gattacgcca atttcccgaa aatcaccctc attgaaaaca
    21721 aaatgaaggt cgatgaaatg gtcaccgtgc gcgatatcac tctgaccagt acctgtgaac
    21781 accattttgt taccatcgat ggcaaagcga cggtggctta tatcccgaaa gattcggtga
    21841 tcggtctgtc aaaaattaac cgtatcgtgc agttctttgc ccagcgtccg caggtgcagg
    21901 aacgtctgac gcagcaaatt cttattgcgc tacaaacgct gctgggcacc aataacgtgg
    21961 ctgtctcgat cgacgcggtg cattactgcg tgaaggcgcg tggcatccgc gatgcaacca
    22021 gtgccacgac aacgacctct cttggtggat tgttcaaatc cagtcagaat acgcgccacg
    22081 agtttctgcg cgctgtgcgc catcacaact gattaaaagg caggaaccat ggagcgcaac
    22141 gtcacgctcg attttgttcg cggcgtcgcc attctgggga tcctgctatt aaacatcagc
    22201 gcctttgggc taccaaaggc ggcttatctc aatcccgcct ggtacgatgc tattacgccg
    22261 caggatgcat ggacctgggc atttctcgat ctcatcggcc aggtgaaatt cctcacgctt
    22321 tttgcgctgc tgtttggtgc tggcctgcaa atgttgctgc cccgtggcag acgctggatc
    22381 cagtcgcggt taacgctgtt agtcttgttg ggctttattc acggtttatt gttctgggac
    22441 ggcgatattc tgctggctta cgggctggtg gggcttggcg ttttgctgtt attggggctg
    22501 atttccgaca gccagaccag ccgcgcctgg acgccggatg catcggctat tttgtatgaa
    22561 aaatactgga agcttcacgg cggcgttgaa gcgatcagta atcgtgccga tggtgttggc
    22621 aacagtttac tggcactggg cgcacagtat ggctggcaac tggcagggat gatgctcatt
    22681 ggtgcggcgc tgatgcgcag cggctggctg aaagggcagt tcagcttacg tcactatcgt
    22741 cgtactggtt ttgtgctggt ggcgattggg gtgaccatta accttcctgc catcgccctg
    22801 caatggcagc tggactgggc gtatcgctgg tgtgccttct tacttcaaat gccgcgggaa
    22861 ctgagtgcgc cgtttcaggc gattggctat gcgtcgctgt tttatggctt ctggccgcaa
    22921 ttgagccgct ttaagctggt gcttgcgatc gcctgcgtcg gacggatggc gctgaccaac
    22981 tatctattgc aaacgctgat ttgtaccacg cttttttacc acctcggttt gtttatgcat
    23041 tttgaccgcc tggagctgct ggcgtttgtt attccggtat ggctggcgaa tatcctcttc
    23101 tctgttatct ggctgcgtta cttccgccag gggccggtgg aatggctctg gcgtcagtta
    23161 actttgcgtg ctgccggacc ggcaatatct aaaacatcaa gataacgatc tggattacat
    23221 tcgttaacaa aacggctgta accgtttcca ttgctgtgac tcgattcacg aagtcctgta
    23281 ttcagtgctg acaaaatagc cgccagcaag cagtcattta ctgcaatctc ataacaggta
    23341 gtgaatatga tcaccattcg tgatgtagcg cgtcaggctg gcgtctctgt ggcaacggtt
    23401 tcccgggtgc tcaataacag cacgctggtc agtgccgaca cgcgtgaagc agtaatgaaa
    23461 gccgtgagtg agctggatta tcggccaaac gccaatgccc aggcgctggc aactcaggtt
    23521 agcgacacca ttggcgtggt ggtgatggac gtttctgatg cgtttttcgg cgcgctggta
    23581 aaagcggtgg atctggtcgc ccagcagcat cagaaatacg tgctaatcgg caatagctat
    23641 catgaagcgg aaaaagagcg tcacgccatt gaggtgttaa ttcgccagcg ttgtaatgcg
    23701 ttgattgttc actcaaaagc attgagtgac gatgaactgg cgcaatttat ggataacatt
    23761 cccggtatgg tgttaatcaa ccgcgttgtg ccggggtacg cccatcgttg cgtttgcctg
    23821 gataatctca gcggtgcccg aatggcgacg cgcatgttgc tgaataacgg tcatcaacgt
    23881 attggttatc tttcttccag ccacggcatt gaagatgacg ccatgcgtaa agcaggctgg
    23941 atgagtgcgt tgaaagagca ggatattatt ccgccggaaa gctggattgg cactggtacg
    24001 ccggacatgc cgggcggtga ggcggcgatg gttgaactgc tggggcgcaa tctacaactt
    24061 accgctgtat ttgcttataa cgacaatatg gccgctggcg cactgacagc attaaaagat
    24121 aatggcattg cgattccgtt acatctctca atcatcggtt tcgatgatat tcccatcgcc
    24181 cgttacaccg acccgcaatt aacgaccgtg cgttatccca ttgcttcaat ggctaaatta
    24241 gccaccgaac tggccttgca gggggcagca ggcaatattg atcctcgtgc cagccactgt
    24301 tttatgccga cgttagtgcg tcgccattct gtcgcaacgc gccagaatgc ggcggcgatc
    24361 actaactcaa caaatcaggc gatgtaaccg ctttcaatct gtgagtgatt tcacagtatc
    24421 ttaacaatgt gatagctatg attgcaccgt tttaatgttg taacccgtat gtaacagtga
    24481 ataatcactt tcaccgaggt aatagcgtca taacaacaat taaagccgtt ttctggagcg
    24541 ttaccgggca tggaagaacg aattttaaaa agtgagcttc ggcgttcagt aacacttcat
    24601 taactctact gccccgccga gcatttatct caagcactac cctgcataag aaaaaccgga
    24661 gataccatga ataagaaggt gttaaccctg tctgctgtga tggccagcat gttattcggt
    24721 gccgctgcac acgctgctga tactcgcatt ggtgtaacaa tctataagta cgacgataac
    24781 tttatgtctg tagtgcgcaa ggctattgag caagatgcga aagccgcgcc agatgttcag
    24841 ctgctgatga atgattctca gaatgaccag tccaagcaga acgatcagat cgacgtattg
    24901 ctggcgaaag gggtgaaggc actggcaatc aacctggttg acccggcagc tgcgggtacg
    24961 gtgattgaga aagcgcgtgg gcaaaacgtg ccggtggttt tcttcaacaa agaaccgtct
    25021 cgtaaggcgc tggatagcta cgacaaagcc tactacgttg gcactgactc caaagagtcc
    25081 ggcattattc agggcgattt gattgctaaa cactgggcgg cgaatcaggg ttgggatctg
    25141 aacaaagacg gtcagattca gttcgtactg ctgaaaggtg aaccgggcca tccggatgca
    25201 gaagcacgta ccacttacgt gattaaagaa ttgaacgata aaggcatcaa aactgaacag
    25261 ttacagttag ataccgcaat gtgggacacc gctcaggcga aagataagat ggacgcctgg
    25321 ctgtctggcc cgaacgccaa caaaatcgaa gtggttatcg ccaacaacga tgcgatggca
    25381 atgggcgcgg tagaagcact gaaagcacac aacaagtcca gcattccggt gtttggcgtc
    25441 gatgcgctgc cagaagcgct ggcgctggtg aaatccggtg cactggcggg cacagtactg
    25501 aacgatgcta acaaccaggc gaaagcgacc tttgatctgg cgaaaaacct ggccgatggc
    25561 aaaggtgcgg ctgatggcac caactggaaa atcgacaaca aagtggtccg cgtaccttat
    25621 gttggcgtag ataaagacaa cttggctgaa ttcagcaaga aataagactg attgggcgca
    25681 gtcgtttact gcgcccttat tgtatggcca acaaggtaaa attatatggt cagctcaacg
    25741 actccgtctt ccggggaata cttgttggaa atgagcggta tcaacaagtc ctttcctggt
    25801 gttaaggcac ttgataacgt taatttaaaa gtccggccac attctatcca tgcattaatg
    25861 ggggaaaacg gtgcaggaaa atcgacatta ttaaaatgcc tgtttggtat ttatcaaaaa
    25921 gactccggca ccattttatt ccagggtaaa gagatcgatt tccattccgc caaagaggca
    25981 ctggaaaatg gtatttcgat ggtacaccag gagttaaacc tggtattaca acgttcggtg
    26041 atggataaca tgtggctggg gcgatatccc accaaaggca tgtttgtcga tcaggacaaa
    26101 atgtaccgcg aaaccaaagc gatttttgat gaactggata ttgatatcga tccgcgtgcg
    26161 cgcgtcggca cattatccgt ttcgcaaatg cagatgatcg aaatcgccaa agcgttttcc
    26221 tataacgcga aaattgtgat tatggatgaa ccgacttctt cgttaaccga aaaagaggtc
    26281 aatcatctgt tcactattat tcgtaaatta aaagagcgcg gctgcggtat tgtttatatc
    26341 tcgcataaaa tggaagaaat cttccagtta tgtgatgaag ttaccgtatt gcgcgacggt
    26401 cagtggatcg ccaccgaacc gctggcagga ctgacgatgg acaagatcat cgccatgatg
    26461 gttgggcgtt ctcttaatca gcgttttcct gacaaagaaa acaagccggg cgaagtcatc
    26521 ctcgaggtgc gtaacctaac gtcactgcgc cagccgtcga ttcgcgatgt ctcgtttgat
    26581 ctgcataaag gggagatcct cggtattgcc gggctggtgg gggcgaaacg taccgatatt
    26641 gttgagacgt tatttggtat tcgcgagaaa tcggctggca ccattacgtt gcacggcaaa
    26701 cagatcaata accataatgc caacgaagcc ataaaccacg gatttgcact ggtaactgag
    26761 gagcgccgct caacgggaat ttatgcctat ctggatattg gttttaactc gttaatttcc
    26821 aatattcgca actacaaaaa taaagttggt ttactggata actcgcggat gaaaagcgat
    26881 acccagtggg tgattgattc gatgcgggta aaaacgccgg gtcatcggac gcaaattggt
    26941 tcgctctccg gtggtaatca gcaaaaggtg attattggtc gctggctact aacgcaacca
    27001 gaaatattaa tgctcgatga accgacgcgc ggtattgatg tcggggcgaa gtttgaaatt
    27061 tatcagttaa ttgccgaact ggcgaagaaa ggcaagggga ttattattat ctcctctgaa
    27121 atgcctgagt tgttagggat aacagaccgt attctggtca tgagcaatgg tctcgtttcc
    27181 ggaattgtcg atacaaaaac aacaacgcaa aacgaaattc tgcgtcttgc gtctttgcac
    27241 ctttaagatt aggggcttcc catgagtgcg ttaaataaga aaagttttct tacttacctg
    27301 aaagagggcg gtatttacgt cgttctttta gttttgctgg cgattattat tttccaggac
    27361 ccaacatttt taagtctgtt gaacttaagt aatattctca cccagtcatc ggtgcgtatt
    27421 attatcgcgc tcggtgtggc agggttaatt gtcacccagg ggaccgatct ttctgctggt
    27481 cgtcaggtag ggctggcggc agtggtggct gcgacattat tgcagtccat ggataacgcc
    27541 aacaaagtgt tcccggaaat ggcgacgatg ccgattgcgc tggttattct gattgtctgt
    27601 gccattggtg cggtgatcgg tttgatcaac ggtctgatta tcgcttatct caacgtgacg
    27661 ccgttcatta ccacgctcgg cacgatgatc atcgtctatg gcatcaactc gctctattac
    27721 gactttgtcg gggcgtcgcc aatttctggt tttgacagtg gcttctctac ctttgctcag
    27781 ggctttgtcg cgctggggag tttccgtctc tcttacatca ccttctacgc gttgattgcg
    27841 gtggcgttcg tctgggtgtt gtggaacaaa acccgcttcg gtaagaacat ttttgccatt
    27901 ggcggtaacc cggaagcggc aaaaatatct ggtgtcaacg tcggcctgaa cctgctgatg
    27961 atctacgcgt tgtctggcgt gttctatgcc tttggcggga tgttagaagc cggacgtatc
    28021 ggctctgcca ccaacaacct cggctttatg tatgagctgg atgctatcgc ggcgtgcgtg
    28081 gtaggcggtg tatcgttcag cggcggtgtg gggacggtga ttggcgtggt gaccggggta
    28141 attattttta ccgtcatcaa ctatggcctg acgtatatcg gcgtaaaccc atactggcag
    28201 tacatcatca aaggggcgat tattatcttc gccgtagcgc tggattcact gaaatacgcg
    28261 cgtaagaagt gatgtgattg cggatattgc cggatgcgtc tctggtttta caacgttacc
    28321 gggtgttctt tctcgccttt cttaaacttc acttccccga gctcaataca acccaccggg
    28381 cagacgtgac cacaaagcag acaacccaca catttctcgg tattacaatg cggtgtgcgg
    28441 gttttctcgc tccattccat cgcctggtga ccgccgtcgt aacaggaaat ataacagcgt
    28501 ccacagccaa cacatttgtc aagattgata cggggataga caatataact gcggtctaaa
    28561 tcttccgccg ggacaatatt gttattcgcc agacctacca tctcctgcaa cgaatcaaat
    28621 ccctgatcgg cgagataatg cgataacccg ctcgccatat cttctacgat gcgatagccg
    28681 tactgcataa tgccggtggt gacctgtaac gttgctgcgc cgagcaataa aaactccgcc
    28741 gcatcttccc aggtttcaat gccgccgata ccgctgattg ggaaatcgcg cagttctgga
    28801 tgggtgcgca tttgctgaat gaagcgcagg gcgatcggtt ttaccgcttt accggaatat
    28861 ccggaaatac tcgatttacc gttaacgatc ggcataccga ttttctgatt aagatcgata
    28921 ttggtgatgg atttaacggt gttaattgcc gcaatgccat ctgcgccgcc gcgcttcgcc
    28981 gccagcgcca cttcgcacat atcaccgata ttcggcgtca tcttcgccag cattggcagc
    29041 gtggaacccc gtttcactgc ccgacaatat ttttctacca gctccgggct ttgcccgaca
    29101 tcgctaccca tcgcatgaga agtcatttgc ggacaggaga agttacactc gatcatatcc
    29161 gcgccagctg ctcccattgc tgctcatttt cccccatgat cgaagcgatc aatactttgt
    29221 ccgggtaatc ttccttcagc cgacgcaggg cggtcagatt ctcttccaac ggatgttcag
    29281 caatctgctc catatttttg aagccgataa aaccggtatc ttctttcacc agatgatcaa
    29341 aacgcggcga gacttcgttg gcgataaaaa agccgatcgt tttaaacacc acaccgcccc
    29401 aacctgtgtc gtaggctttg gcacacatct catagcagtt gcctaccggc gaagaagaga
    29461 ggcagaacgg gttgggaaag ttcacgccgc aaaaagtaat cgaaagatct ttcgttaaca
    29521 tgagcaagct ccctctaaat agtgatgaat cgcctcggcg gcttctttcc cggttttcac
    29581 ggcatagacc acggttttgt caccctcaac aatatcgcca gcagcaaaga cttgcggatc
    29641 gcggatctga taattttgtg ttttgatggt gttacgctgc tgctctaact ccgcaaaggc
    29701 atccagtctg gcatgctgac cgacggcgag aataatttta tctgccgcca tcgtcagttc
    29761 gcctgataac cgtacatgct taaacgtgac tttattgcct tccacggcta ctggcgtgaa
    29821 tccatcgatg atcgaaacgc ccagctcccg ggcgctggca aactcttttt cgcttgccgg
    29881 aaactcatct aactcttcac gtgctacgca agttaccgcc tgacagccga gaactttcag
    29941 cgtgctggct acgtccatcg cgacatcacc gccgccgata attaatgcgc tttgtggaat
    30001 gctgatatcg ccttgcgcct gtcgtacacg ttgcaggaag tcgacggcaa tctcaacgtc
    30061 gctatgctca aacagcggta gcccggaacc gcttgataac cccacggtga ccagtaccgc
    30121 gcggttttcc gctttcagtt gctccagggt gagtgtttta ccgatttcgt tattgcactt
    30181 aatggtcacg cccatttttt caatacgggc gatctctgca tccagcactg attgcggtaa
    30241 ccggaattgc ggaataccgt tacgcaacca gccaccgggg tgcgcttctt tctcataaat
    30301 cgtgacgtca taaccctggt ttgtcagtgt cacactggcc tgcaatccgg caggacctgc
    30361 gccaataatc gcgactttgc cgagcgtttt agtaccgggc tgataaattt ccatcccggt
    30421 ttgttgttca aaatcagtaa caaaacgctg taagcggccg atatcaatgg gagcatcaac
    30481 accggcacgg gtacaaccgc tttggcataa tttctccgtc gggcacactc tggcacaaac
    30541 ggcaccgagg gcattatttt cgcgaattgt ctcggcagcg cctttaaaat tacgaaagta
    30601 gattgagcga ataaatttcc ccggatcggt ctgtgccggg caagcctgac tacagggagc
    30661 gtcgtgacat aataaacagc gagaggcttc tttaatcgcc agtaacggcg taaaagccgg
    30721 agtgagttca tccagataat tttgttgcgg catgtgatct tccttaatgc tatgagtaat
    30781 tatggcgaac gctaaataaa tattgcgtgc tgatattaag gctatgcgcg tggggaaatt
    30841 taagctgtga aaggattcac aaatagctgc ggttcagaat gttatatccg cagcttattg
    30901 agtgaagtaa taattattct caataattaa cagaaaagag aaaatattaa cgatagaaaa
    30961 taagataaac aatcatgccg atagataaaa ttccgaagcc aatatatgcc accatataag
    31021 cgaagaagag tccgggattt tttgctcgcc agacatacac cggttccggt gcatttttaa
    31081 cccgtttatc aatcgcgcct gcgcgccagc ctttccatgc ttcgcggaaa caaaatatcg
    31141 ggataaggaa aaaaacggca acgacaaaaa actgctgtac atccataact actttccttg
    31201 tttcttttgt aattcaagta actgttcggg tgtaacggcg ggatacccct gcgcatcttc
    31261 cggcacctgg tgcatagccg gaatagggac cagcggccct aaaaaacgcg gttcacgttt
    31321 aaaaatataa aggtcagcca gcgcaccgaa acgggcggca aattcgcgaa tacgtactga
    31381 cagcatatct ttcggtgacg gtacggcata acactgagcc tgaatcccca tatgcagcgc
    31441 aataaataat gctcgctcac agtggaaacg ttgggtgata ataatgaaat cattagtatc
    31501 gaaaactttg cgtgtacgca cgatggaatc cagcgtacga aagcctgcgt aatcgagaac
    31561 aatatctgat gggtcgacac cagcagcgat taaatctttg cgcatggtca tcggctcgtt
    31621 ataactttgc aatgcgttat cgccgctcag taatagataa tttaccttac cgctgttata
    31681 ggcattaatc gctccttgaa tgcggtagcg ataatactga ttaataacgc cggtacgata
    31741 atattttgct gtgccgagca ccacgccgac ctggcggtag gggagatcct gcaattcgtc
    31801 gtagatataa ggcgcggttt tccagctcat ccagcgatcg aggccgagca cagtcaacag
    31861 cagcaagccg atcaggacta acaggctgag gaacacgcgc tttaacatgc aggagactcg
    31921 agctaaggaa aatttccatc aggctacttt acccgctggt tttgcgcaag aaacggcaaa
    31981 ctgtttgccg ggaatttcga caccaggaga gggctggcaa gccggacaag gcgttcacgc
    32041 cgcatccggc accaggctta agcgagaagc acgcggtcga tactgtgaca gccgagagct
    32101 ttcagcgttg cggaggtggc atcccactga atcaacggcg catcggcttt ttctgccaga
    32161 accgcgcgct ggatatccgg gtaatcataa cccttgagat tcaacagaat taacgccccc
    32221 tgcaacggcg gcagagtcgg gttgaatgcg gcgttttcag cgtagctgcc gctgaaaata
    32281 cgaccgtctt tacattccag cgcgacaccg cttggtgact tactgtaagg catgtgcgaa
    32341 cggtttgccg ctgcaatcgc cgcctgagaa agcgcatcac cagtcagcgc atagccgtga
    32401 tcctgctcgt ccatcagcag cgttttaatc tccagatctt tcggcccaaa ggcatccggc
    32461 agatagtcac gcagcgcgtg tgcctcgcgg cccggcaaat gaatacgcag atccagaccg
    32521 ctgttcagtt cattcataaa ctgacggcag tgaccacaag gcgtatagtt aacggtgatg
    32581 gctgcaagcg ctttttcacc actcaaccaa gcgtggctga tcgcgctttg ttcggcatga
    32641 acggtttgct gcattgttgc accaataaat tccatattgg caccaaaata ccaggttccg
    32701 ctcacaccgc gcgcaattgc gccaacatta aaattcgaca atggcgtacg cgcacaggcc
    32761 gccgccagcg gaagtagtgc gaatgccagc gcgtcttcgt ccagtcccgt tgcgctcttc
    32821 agcgatgaga cttgctcccc ggtcaacaaa gcggggaagt acttgtctgc cagaataggt
    32881 tccagtgcag attgcaagtt atccgcaagt tgggcaaaag cggtttgaaa acgtggatgc
    32941 atggcgttgc ctcataatca tgtaatggga taacagttta cgaacccgtt atggctttat
    33001 atgtgatctg aatctcatta attatgcaac acgtgaaact tacatcaaaa atgcgcgacg
    33061 tatcgcaaat tttagcccat tactgccaga ataatcggga aaaggaacgg tgcgatcagc
    33121 gaagtaatta tcccgcataa caccagcgct agcgaactaa atgcaccttc ctgataatcc
    33181 agctcggcgc agcgcgccgt accgagggcg tgcgaggcag tccccattgc cagaccgcgc
    33241 gcagctttgg tacgaatacg catcgcatta agcaatgtat ggccaaatac cgcgccgagg
    33301 atgccgacga aaatcacgca aactgcgctg attgccggaa taccgccaat actgccgcca
    33361 accgccattg caataggcgt ggtgactgat ttcggcagga ttgacgcggc gatttccggt
    33421 gaagcgccca tcaacaatgc cacggaagtc cccgtcacca tcgcaaccac gctgccgata
    33481 aaacagatgg tgatgatcga tttccagcgc gcgcggatct ggtgtagctg ctcatataaa
    33541 ggataggcca gcgcgacgac cgccggttgc agcagatcgt ttagcacttc gctaccttta
    33601 aagtaactgt cgtaagagat gccggtcagc attaaaaaag gaatgatcac caccattgct
    33661 accagcaacg ggttaagcag gggaaactta taccgtgccg ccagtttgcg ggcggcaaaa
    33721 aagacaatca acgtcagcgg taatgaccac cagatattcg ccatcattct tctgatcctt
    33781 tctgacctac gactttacgt tcaccgtgta ccagttgtga actccagctc accaccagaa
    33841 aaaccaccag cgtactgaca gcacaggaaa ccactaccgg gccaaactgc gcgcggagca
    33901 aatcaaaata ttgcatgacg cctacgccaa tcggcacaaa caatagcgcc atatagcgaa
    33961 tcagtacgta gcaccccgga ttgacccatt tcgccggaag aatttgcaag gccagcagga
    34021 caaacaggat cagcatcccg atgatgctgc ccggaatggt taccggtagc agggaagcaa
    34081 tgaaaatgcc tgcatacagg caggcataaa tcaggacgaa agcgcgtaaa tattgccaga
    34141 taatgttcag tgtcttactc ataatcaaca ccccagatga gatggattca tcatacaatt
    34201 aattcgtaaa acgtgctacc gatcacatta agaaatcagc gcttattttt tcgctacccg
    34261 gtattacgca atgagcagcg aactcaggca tacatcaagc atagagaggg attgcagttt
    34321 gcagcaatcc ctctttgcag acctttaatc cgctgaccat aggcgctaac atagcgcctc
    34381 atttttttgc gggtgatgat atgcgtgtgt tactggcacc gatggaggga gtgcttgact
    34441 ctctggtgcg tgaattgctg accgaagtta acgactacga tctgtgcatc accgagtttg
    34501 tccgcgtggt ggatcaactg ctgccggtaa aagtctttca tcgcatttgc cctgagctac
    34561 aaaacgccag ccggacacca tctggtacgc tggtgcgcgt gcagttgtta ggtcagttcc
    34621 cacaatggct ggcagagaac gccgcccgtg cggtggagtt aggtttctgg ggcgtggatc
    34681 tcaattgcgg ctgcccgtcg aaaacggtta acggtagcgg cggcggggcg acgttactca
    34741 aagatcctga actcatctac cagggggcaa aagcgatgcg tgaagctgta ccggcgcatt
    34801 tgcctgtcag cgtgaaagtg cgtctgggct gggacagcgg tgagaagaaa tttgaaatcg
    34861 ccgatgcggt tcaacaggct ggcgctacgg agctggtggt gcatgggcgg acgaaagagc
    34921 agggttaccg cgcggagcat attgactggc aggcgattgg cgagattcgc cagcggctga
    34981 atattccggt gattgccaac ggtgaaatct gggactggca gagcgcgcaa caatgcatgg
    35041 cgatcagcgg ctgcgacgca gtgatgattg gtcgcggggc gctcaatatt cccaacctga
    35101 gccgggtggt aaaatataac gaaccgcgaa tgccgtggcc ggaggtggtt gctttgctgc
    35161 aaaaatatac ccgtctggaa aagcagggcg ataccgggtt atatcacgtt gcgcggatta
    35221 aacagtggtt gagttatttg cgtaaagaat acgatgaagc aacggaatta tttcagcatg
    35281 ttcgggtgtt gaataactcc cctgatattg caagggctat tcaggcaatt gatatcgaga
    35341 aactctaaaa accatagtga tgtttttgac aatgtgttag ctctttatca ataggccgca
    35401 cggttgtttg gtggcgtcaa aatcgccgag gcgttccctg aaggccgggg cagcccacat
    35461 ggatgtgggc tgagggcgcg ttttacaggg atgttacctc gcgcccgacc cggtagccgt
    35521 aagggataag ccgagggcac cgcgcagcgg cgattttgtt cgccagagcc cgggggggca
    35581 gggggcggcg gcgattggtc gccccctgcg cgctccttgc gccagtgatt atatgaagca
    35641 atgcacatga caggagcgca acaggatgag agactgaaat attctcgtta ttttcggatc
    35701 tgatgacatt aattaatttt aaaatatcat cttaaatacg ccagtcacca ccagtagccc
    35761 aatcaggaaa ataatcaata cagcccagag tataattttc attggcgttc ctttatttgt
    35821 gagtgctgaa taagtatagg aaaaaacgaa gccctttgcc gttatccata aacgattaag
    35881 ataaatcgga agatatcatt tacttagtct atatttttct gggtatgtcg ttttattgct
    35941 actgatgtat tgccacgcga aactaaaaaa tatatcgcaa cacacggtaa tctccgcgca
    36001 ccgtttctta cctgattatt cccccatgaa tcgtgattcc ttttatccag ccatcgcctg
    36061 ttttccgctg ttactgatgc tggccgggtg tgcgcctatg catgaaaccc gccaggcgtt
    36121 aagccagcaa acgcccgctg cacaagttga caccgcatta cccacggcgc tgaaaaatgg
    36181 ttggccagac agccaatggt ggctggagta tcacgataat cagctcactt cattaattaa
    36241 caatgcacta cagaacgcac cggatatgca ggtggctgaa cagcgcattt agctagctga
    36301 agcccaggcg aaggcagttg ccactcagga tggtccgcag atcgactttt cggcggatat
    36361 ggagcggcaa aaaatgtcgg cagaagggct aatggggccg tttgctctga acgatccggc
    36421 cgcaggtacg accggtccgt ggtacaccaa cggaactttt ggcttaacgg cgggctggca
    36481 tctcgatatc tggggaaaga atcgggcgga ggttactgcc cgcctgggta cggttaaagc
    36541 acgggcggcg gaacgcgagc aaacccgcca attgctggct ggcagcgtag cccgcctgta
    36601 ctgggagtgg caaacccagg cggcgttaaa cacggtcttg cagcaaatag aaaaagagca
    36661 gaacaccatt atcgcgaccg atcgccagct atatcagaac gggattactt cttcagttga
    36721 cggtgtggaa accgatatta atgccagcaa aacccggcag cagctcaacg atgtcgcggg
    36781 gaaaatgaaa attattgagg cacggttaaa tgcactcaca aatcaccaga ctaagtcatt
    36841 gaagcttaaa ccggtcgcgt tgccgaaagt ggcaagccag cttcctgatg aactggggta
    36901 ctccttactg gcccggcggg cagatttgca ggcggcgcac tggtacgttg agtcatcgtt
    36961 aagcactatt gatgcggcaa aagcggcatt ttatcctgac atcaacctga tggccttcct
    37021 gcaacaggat gcgttgcact taagcgatct gttccgtcat tccgcgcagc aaatgggcgt
    37081 tacggcaggc ctgacgctac ctattttcga tagtggtcgt cttaacgcca atctcgatat
    37141 cgcaaaagcc gaaagcaact tgtctatcgc cagctacaac aaagcggtgg ttgaagcagt
    37201 gaatgacgtg gcgcgggcag ccagtcaggt tcagacactg gcggagaaaa accagcatca
    37261 ggcgcaaatt gagcgcgatg ccttgcgtgt ggtaggtctt gcgcaggccc gctttaacgc
    37321 gggcatcatt gctggttccc gcgtcagcga agccagaatc ccggcgctgc gtgagcgggc
    37381 caatggcctg ttattgcaag ggcagtggct ggatgcctcc attcgactca ccggtgcgct
    37441 gggcgggggt tacaaacgct gatgatatgc gccttctata cttaacgttt attcagcgtt
    37501 aagtggagaa ctcgatggca taggtcgcga taattaccgc ttccgattcg gggatcggca
    37561 aagagtgcgc gttattactg gcgcagcagg ggtttgatat tggcattacc tggcactcag
    37621 atgaagaagg ggcaaaagat accgcgcgtg aggtggttac ccacggcgta cgtgcggaga
    37681 tcgtgcaact ggatctcggc aatctgccag aaggtgcgca ggcgctggag acactcatcc
    37741 agcgatttgg gcgtattgat gtgctggtga ataatgcggg tgcaatgacc aaagcgccgt
    37801 ttcttgatat ggcttttgat gagtggcgca agatttttac cgttgatgtt gatggtgcat
    37861 ttttatgctc gcaaattgcg gctcgtcaga tggtgaaata agggcagggc ggtcgcatca
    37921 tcaacattac gtcggtacat gaacatacgc cgctgccgga tgccagcgcc tacacagccg
    37981 ctaaacatgc gctcggcggg ttaaccaaaa cgatggcgct ggagttggtc aggcataaga
    38041 ttttggtgaa cgcagtcgcg cctggggcga tagccacgcc aatgaacagc atggatgata
    38101 gcgacgtgaa gcccgacgcg gagccttcga ttcctctacg gcgttttggc gcaacgcatg
    38161 agattgccag cctggtggtg tggctttgtt cggagggcgc aaattacacg acagggcagt
    38221 cgttgatagt ggatggcggc tttatgttgg cgaatccgca gttcaaccca gaatagcgca
    38281 gtgaggtgcg tggatatttc aatgggttag gccagataag acaccagcgt cgcatcaggc
    38341 atggaacacc cggtgccgga tgtggagtga atgcttatct gtggcaactg ctttacgcct
    38401 gattatccgg ctttttcttc ccgcgccgtt tcagccacca gcgcacgccc accaccagaa
    38461 ccacgaccag aatcaaccag acccagtgct tcaaatgctg gtcgagattg tgcaaccacg
    38521 gcgcaatcac ctgaccacca gcgtaaccaa tagtggtaaa aatcaacgcc caggcaaatg
    38581 cgccgagaat attcagcggc agaaagattt tcggcggcag ctggctggca ccaatcagcg
    38641 tcgggccaat cacccgaaag ccatacataa agcgcgtacc aatgacaaac agatacggat
    38701 ggcgttggat aagtttctgt gcccgctcaa ttttatcctg atgtttcgag aaacggcgta
    38761 acagcttgcc gccaaaccgc cgcccgcaca gatagagcac ctggtcgcca atcatgccac
    38821 caagcgccac agaaagtacc accagtggga actttaatag cccctgatgt gccgcaacgc
    38881 ctcccagcaa agtcacggtt tcaccttccg ccaggctacc gataaccagc gcggcataac
    38941 catattgtga gatgagtgta ttgagatcca taggccagtt tgtctccctt tggttgcctg
    39001 taagcataca ccctgtgaat cattcacgct gaaaaaggac atacatcaga cggtgcaaat
    39061 tacatggatg aacaaaaaat aatcacaagc tggcttatac ttgaactgtc aggttcatga
    39121 actcacaaca agggggcggt atgagccatg tctggggact gttttcccat cccgatcgtg
    39181 aaatgcaggt gattaatcgc gaaaacgaaa cgatttctca ccattacacc caccacgttt
    39241 tgctgatggc ggcgattccg gtgatttgcg ccttcattgg cactacacag attggctgga
    39301 attttggcga tggcactatc ctgaagttat cctggtttac cggactggcg ctggctgtct
    39361 tgttttacgg cgttatgctg gctggggttg cggtgatggg gcgggtcatc tggtggatgg
    39421 cgcgcaatta cccgcagcgt ccgtctcttg cgcactgcat ggtcttcgcg ggctacgttg
    39481 caactccgct gtttttaagc ggtctggtgg cgctttatcc actggtctgg ttgtgtgcgc
    39541 tggtcggcac ggtggcgctg ttttacaccg gctacctgct ttaccttggt attccgagct
    39601 tcctgaatat caacaaagag gaaggcctta gcttctccag ttcgacgctg gcgattggcg
    39661 tgctggtact ggaagtgctg ctcaccctaa ccgttatact atggggctac ggttatcgtt
    39721 tgttctagaa agttctttga cgttgcattg ctggcgtaaa tgtcagcaat gcagcattta
    39781 ctcacgattc tcctttgacg atcgcacttt ttgctcgtta tgatggcgtc gctagcctca
    39841 gtaaatcctt actccggcgg tgcgcaaccc gtgcgcgtga accactatct gaatgctcat
    39901 catgccgaaa tttcgagttt ctttatttag cttggccctg atgctggctg tgccttttgc
    39961 accgcaggcc gttgcgaaaa cggcagccgc taccaccgct tcacaaccgg aaattgcctc
    40021 cggtagcgcg atgattgttg atctgaatac caacaaagtg atctattcga accacccgga
    40081 tctggtgcgt ccgattgcgt ctatcagcaa attaatgacc gcgatggttg tgctggatgc
    40141 acgactgccg ctggatgaaa aactaaaagt ggatatcagc cagacgccgg agatgaaagg
    40201 ggtctattcg cgcgtacgac tgaatagcga aatcagccgt aaagatatgc tgttgctggc
    40261 gctgatgtct tcagaaaacc gcgcggcggc aagccttgcg caccattatc ccggtggtta
    40321 caaagccttt attaaggcaa tgaatgcgaa agcgaaatcg ctcggaatga acaacacgcg
    40381 ctttgttgaa cctaccggat tgtcggtgca taacgtttca actgcccgtg acttaaccaa
    40441 actgctcatt gccagcaaac aatatccgtt gatcgggcag ttaagtacta cccgggaaga
    40501 tatggcaacc ttctctaacc cgacgtatac gctgccgttc cgcaatacta atcatctggt
    40561 gtatcgcgat aactggaata ttcagttaac caaaaccggc tttaccaatg cggcgggcca
    40621 ttgtctggtg atgcgtacgg ttatcaataa taaaccggtg gcgctggtag tgatggacgc
    40681 gtttggcaaa tatacccatt ttgccgatgc cagccgcctg cgtacctgga ttgaaaccgg
    40741 taaagtgatg cctgtgccgg cagcagcgtt gagctataaa aaacaaaaag ccgcccaaat
    40801 ggcggcggcg gggcagacgg cacagaacga ttaagtgaat tcggatggcg atactctgcc
    40861 atccgtaatt tttactccac ttcctgccag tttttccgtt tactggtttt accgatcccc
    40921 ggattcatgc tattggtcgg atcattctcg cgatagaact tctgcaacgt ctccggtgct
    40981 ttatacaaat gaccgacgtt atgctcggca gggtactgcg cgccgcgctg ctgtagcagt
    41041 tccagcatct gttcttttaa cgcatgcaca tccacgcctt ttttcactat gtaatcctga
    41101 tggaagacat agcacataaa atggccgtaa tagagcttgt gcaccagctg gctgtcgatc
    41161 tccggcggta aatgctcata ccactcggtg tcgttacgcc gcagagcgat atccagtgcc
    41221 agaatgtctt cgacttcatc ggaatgcacc gcctgataac gaattgctgc gcccgcagcg
    41281 gcgaaacggt gtaaaaacgc tttgctacct tcctccggcg tacagacaaa gaaatcgccc
    41341 tcggcctgtt tgaaataatc caccagccac gatttggctt caccgacgcc atcgcctgcc
    41401 atttttaaca gcagatgatg ctcgtattta tcgcgccagt ttttcatgcg cggcggtaaa
    41461 tggctgggga acaggtgacc gaatttttgc attgcacggt cagtaaaatg cggacggaag
    41521 aatttcactt tctccagcat cgcatcggtg cgtcccttga gattaaagaa gaacggcatc
    41581 ttgtcggtgc ctaacttatc aatcatcagg aaggtgtctt tgccgtattt ttccgcaata
    41641 tcgtagatat cccggtgcat atattccccg gcaaccggca gattttcgaa gttagccaga
    41701 atatgacggc ggatttcggt cagcacttcc ggctggttgg tgccgatata aaacacctgc
    41761 tgattttttt ccgcttcgaa ggtatcaaga cgtaccgcaa agacggccag cttcccggcg
    41821 caaccagaag attcaaataa ccgatccgga tcggcgttat aacgtgcggg cgtgtcggct
    41881 tcaatatcac gaacgcggtg gacatagtca taatcgtggg cgtgacgacc atcgtgacgc
    41941 acatcgtcat ctttgatgcg atcgtcatcc agcttgctaa ggatctgctc cggcgtttcg
    42001 cccagatcaa tccccagatg gttcaccagc gtcagtttgc catcttcatc tatacgcgcg
    42061 aataacgaca tttcggtata cgccgggcca cgttgcacca gcgagccgcc ggagttgtta
    42121 caaataccgc cgatgaccga tgcgcctata cacgatgatc caatcaccga atgcggttcg
    42181 cgtcccagcg gtttgagggc tttttccagc gaatagagcg tggtgcccgg ataggccagc
    42241 acctgttcgc ccttgccgag aacgtgcagc ttgtcgagac gcagggtgct gatgatcacg
    42301 atatcgcgat catagtcgtt accgtttggc gtcgatcctt cggtcaggcc tgtattggca
    42361 gcctgcatca gaataatttt gtcggcggtg acgcaggctt tcagcacccg ccacaattcc
    42421 agtagtgagc cagggaaaac gacagccagc gcgtcgccct gaccagaacg gaagcccttg
    42481 cgatagcggg ccgtttttgc gggatcggtg agcaggtgtg aatgaccgac cagacgggca
    42541 agttcattca aaaaggcttt attatcagtt gttgtcatgg aagacatttt ccactccttg
    42601 tggtggcgaa aaaatatcac aaactagaat agcgccttgt atgacaattc agcgagtgaa
    42661 tattttgaaa attcagacaa taaactgagc attctttttc tctctccatc atgcttatgg
    42721 caaactacgg tttttgcacc attttcgctg ccacgtcggg caacaaagga agaaaaatcc
    42781 atatgaaatg gctatgttca gtaggaatcg cggtgagtct ggtcctgcag ccagcactgg
    42841 cggatgattt attcggtaac catccattaa cgcctgaagc gcgggatgcg ttcgtcaccg
    42901 atctgctcaa gaaaatgacg gttgatgaga aaattggtca gctgcgctta atcagcgtag
    42961 gcccggataa tccgaaagag gcgatccgcg agatgatcaa agacggtcag gttggggcga
    43021 ttttcaacac cgtaacccgt caggatatcc gcgccatgca ggatcaggtg atggaattaa
    43081 gccgcctgaa aattcctctt ttctttgctt acgacgtgct gcacggtcag cgcacggtgt
    43141 tcccgattag cctcggtctg gcctcgtctt ttaacctcga tgcggtgaaa acggtcgggc
    43201 gtgtctctgc ttatgaagcg gcagatgatg gcctgaatat gacctgggca ccgatggtcg
    43261 atgtctcgcg cgatccgcgc tggggacgtg cttccgaagg ttttggcgaa gatacgtatc
    43321 tcacctcaat aatgggtaaa accatggtgg aagcgatgca gggtaaaagc ccggcagatc
    43381 gttactcggt gatgaccagc gtcaaacact ttgccgcata cggcgcggta gaaggcggta
    43441 aagagtacaa caccgtcgat atgagtccgc agcgcctgtt taatgattat atgccgccgt
    43501 acaaagcggg gctggacgca ggcagcggcg cggtgatggt ggcgctgaac tcgctgaacg
    43561 gcacgccagc cacctccgat tcctggctgc tgaaagatgt tctgcgcgac cagtggggct
    43621 ttaaaggcat caccgtttcc gatcacggtg caatcaaaga gctgattaaa catggcacgg
    43681 cggcagaccc ggaagatgcg gtgcgcgtgg cgctgaaatc cggaatcaac atgagtatga
    43741 gcgacgagta ctactcgaag tatctgcctg ggttgattaa atccggcaaa gtgaccatgg
    43801 cagagctgga cgatgctgcc cgccatgtac tgaacgttaa atatgatatg gggttgttta
    43861 acgacccata cagccatttg gggccgaaag agtctgaccc ggtggatacc aatgccgaaa
    43921 gccgcctgca ccgtaaagaa gcgcgtgaag tggcgcgcga aagcctggtg ttgctgaaaa
    43981 accgtctcga aacgttaccg ctgaaaaaat cggccaccat tgcggtggtt gggccactgg
    44041 cggacagtaa acgtgacgtg atgggcagct ggtccgcagc cggtgttgcc gatcaatccg
    44101 tgaccgtact gaccgggatt aaaaattccg tcggtgaaaa cggtaaagtg ctgtatgcca
    44161 aaggggcgaa cgttaccagt gacaaaggca ttatcgattt cctgaatcag tatgaagaag
    44221 cggtcaaagt cgatccgcgt tcgccgcaag agatgattga tgaagcggtg cagacggcga
    44281 aacaatctga tgtggtggtg gctgtagtcg gtgaagcaca ggggatggcg cacgaagcct
    44341 ccagccggac cgatatcact attccgcaag gccaacgtga cttgattgcg gcgctgaaag
    44401 ccaccggtaa accgctggtg ctggtgctga tgaacgggcg tccgctggcg ctggtgaaag
    44461 aagatcagca ggctgatgcg attctggaaa cctggtttgc ggggactgaa ggcggtaatg
    44521 caattgccga tgtattgttt ggcgattaca acccgtccgg caagctgcca atttccttcc
    44581 cgcgttctgt cgggcagatc ccggtgtact acagccatct gaacaccggt cgtccgtata
    44641 atgccgacaa gccgaacaaa tatacttcgc gttattttga tgaagctaac ggagcgcttt
    44701 atccgttcgg ctatggtctg agctatacca ctttcaccgt ctctgatgtg aaactttctg
    44761 cgccgaccat gaagcgtgac ggcaaagtga cggccagcgt gcaggtgacg aacaccggta
    44821 agcgcgaggg ggccacggta gtgcagatgt acttgcagga tgtgacggct tccatgagtc
    44881 gccctgtgaa acagctgaaa ggctttgaga aaatcaccct gaaaccgggc gaaactcaga
    44941 ctgtcagctt cccgatcgat attgaggcgc tgaagttctg gaatcaacag atgaaatatg
    45001 acgccgagcc tggcaagttc aatgtattta tcggcactga ttccgcacgc gttaagaaag
    45061 gcgagtttga gttgctgtaa tgctttagta agggcgcgac gtttgcgccc tttgcctgat
    45121 gttcatatct taacttgctg aattttcgcc ctttgtaggc cggataaggc gctcgcgccg
    45181 catccggcag gaaacgccgg accgaatatg cctcttaagt atttatctga ttctgcaact
    45241 gaatccttcc gctcaggcta actctgtcat tatcaactat gcttttctct taattcgctg
    45301 aaaacaacag taaaagaatg aggaaagcat catgccactc ttaaagctct gggcaggttc
    45361 actggttatg ttggcagccg tgagcctgcc gctacaggcg gcttcccccg ttaaagtcgg
    45421 ttcaaaaatc gataccgaag gcgcgctgct cggcaacatc atcttgcagg tactcgaaag
    45481 ccacggcgta ccgacggtga ataaagtgca actcggaacg actcctgtgg ggcgcggggc
    45541 gattacttcc gatgaactgg atatctatcc ggaatatacc ggcaatggcg ctttcttctt
    45601 taaagatgaa aacgatgcag cgtggaaaaa tgccgggcag ggctacgaga aagtcaaaaa
    45661 gctcgatgcg gagcacaaca agttaatctg gctgacgccc gcgcctgcaa ataacacctg
    45721 gactatcgcc gtgcgtcagg atgtggcaga gaaaaacaaa ctcacttcgc ttgccgacct
    45781 gagtcgttat ctgcaagagg gcggcacctt caaactggca gcctcggcag agtttatcga
    45841 acgcgccgat gcgttaccca cgtttgaaaa agcctatggc tttaagctcg gtcaggatca
    45901 gttgctgtca ctggctggtg gcgacacggc ggtgacgatt aaagccgctg cccagcaaac
    45961 ctctggcgtt aatgccgcaa tggcttacgg cactgacggt ccggtcgcgg cgctggggct
    46021 gcaaacctta agcgatccgc aaggcgtgca acctatctac gcgcctgcac cagtggtgcg
    46081 tgaatcggtg ttgaaggagt atccgcaaat ggcacagtgg ctacagccag tcttcgccag
    46141 cctcgatgca aaaacattgc agcaactgaa tgccagcatt gcagtggaag gactggatgc
    46201 caaaaaagtg gctgccgact acctgaaaca aaaagggtgg gcgaagtaat ttcccgtgac
    46261 ttatctacgt attaatcctg ttctggcgct gctgttgttg ctgacggcaa tcgcagcggc
    46321 gctgccgttt atcagttacg cgcctaatcg tttagtctcg ggtgaagggc ttcatctctg
    46381 gcaactgtgg ccgcaaacga tctggatgct ggtgggcgtt ggttgcgcct ggctgacagc
    46441 ctgttttgtc cccgctaaaa aaggcagcat ttttgcactc attctggcgc aattcgtctt
    46501 cgtattgttg gtgtggggag ctggaaaggc ggcgacccaa ctggcgcaaa atggcagtgc
    46561 gctggcgcgt accagcctcg gcagaggttt ctggctggct gcggcgctgg cattgctggc
    46621 ctgtagcgat gccatccgcc gaatctccac gcatccgctg tggcgctggt tgttgcatat
    46681 gcagattgcc attattccgc tgtggttgct gtactccggc acgcttaacg atctctcact
    46741 aatgaaagaa tacgccaacc gtcaggatgt gtttgacgac gcgctggcac aacatctgac
    46801 gttgctgttt ggtgcggtgc tgcctgcgtt agtgattggt gtgccgttgg gcatctggtg
    46861 ctacttttcc actgcgcggc agggggcaat tttttctctg ctcaatgtca ttcagaccgt
    46921 gccttcggtg gcgctctttg gcctgttgat tgcgccgctt gccgcgctgg ttacggcctt
    46981 tccgtggctg gggaagctcg gcatagcagg aaccggaatg acacccgcac tgattgcgct
    47041 ggtgctctat gccttgctgc cgctggtgcg aggcgtggta gtcggtttga accagatccc
    47101 gcgcgatgtg ctggagagcg ccagagcgat ggggatgagc ggggcgcagc gtttcctgca
    47161 tgttcagtta ccgctggcgt taccggtatt tctgcgcagc ctgcgggtgg tgatggtgca
    47221 aactgtaggt atggcggtga ttgcggcgtt aatcggcgca ggcggttttg gtgcgctggt
    47281 tttccagggg ctgctaagca gcgccattga tttagtgttg ctgggggtga tcccggtaat
    47341 tgttctggcg gtgcttaccg acgcgctgtt cgatttgctt atcgcactgc tgaaggtgaa
    47401 acgtaatgat tgaatttagc catgtcagca aactgttcgg cgcacaaaaa gccgttaacg
    47461 atctcaatct caattttcag gaagggagtt tttcggtgct gattggcaca tctggctccg
    47521 gcaaatccac caccctgaaa atgattaacc gcctggtgga gcatgacagc ggagtgatcc
    47581 gctttgccgg agaagaaatt cgctcgctgc cagtactgga gttgcgccgc cggatgggct
    47641 atgccattca atctattggc ctgttccccc actggagcgt ggcgcaaaac atcgctaccg
    47701 tgccgcaatt acaaaaatgg tcgcgggcgc ggattgacga tcgtatcgac gaattaatgg
    47761 cgctactggg gctggagtca aatttgcgtg agcgttatcc gcatcagctt tccggtggtc
    47821 agcagcaacg tgtgggagtg gcgcgtgcac tggctgccga tccgcaagtc ttactgatgg
    47881 atgaaccttt tggcgcactg gacccggtaa cgcgcggcgc gttgcaacaa gagatgacgc
    47941 gcattcaccg tttgctgggg cgcactattg tgctggtcac tcatgatatt gatgaggcgc
    48001 tacggctggc agaacatctg gtattgatgg atcacggtga agtggtgcag caggggaatc
    48061 cgctgacgat gctgactcgt ccggcgaatg attttgtccg ccagtttttt ggacgtagtg
    48121 aactgggggt gcacctgctt tcgttacgta gcgtggcgga ttacgtgcgt cgcgaagaac
    48181 gggcagaagg tgaggcactg gcagaagaga tgacgctacg cgatgcgctc tccctgtttg
    48241 tcgcgcgggg atgcgaggtg ctgccggtgg tgaacacgca gggccagcct tgcggcacgc
    48301 tgcattttca ggatctgctg gtggaggcgt aagcgtatga agatgttgcg cgatccgctg
    48361 ttctggctca ttgctctttt tgtggcgctg attttctggc tgccttacag ccagccgctg
    48421 tttgctgcct tgttcccaca actgccacga cccgtttatc agcaagaaag ttttgcagct
    48481 ctggcactgg ctcatttctg gctggtggga atttcgagtt tgtttgcggt gatcattggc
    48541 acaggtgccg gaattgctgt tactcgcccg tggggcgcgg aatttcgccc actggtggaa
    48601 actattgccg ccgttggaca gacttttccg cctgtcgcag tgctggcgat tgccgttccg
    48661 gtgatcggct ttggtctgca accagcgatt atcgccttga tcctttacgg tgtgctgccc
    48721 atcctgcagg tgacacttgc cgggctggga gcgattgatc ccagcgtgac agaagttgcg
    48781 aaaggtatgg gaatgagtcg aggtcagcga ctgcgtaagg tcgagctacc gctggcggct
    48841 ccggtgattc tggcgggcgt gcgaacttcg gtgattatca acattggtac ggcgacgatc
    48901 gcctcaacgg taggggccag tacgctgggt acgcccatca tcatcgggct tagcggattt
    48961 aataccgcgt atgtgatcca gggggcgtta ctggtggcac tggcggcgat catcgcagac
    49021 cgcctgtttg aaaggctggt gcaggcgctt agccagcacg caaaataaag gtataacctg
    49081 cgagcatg
//
LOCUS       DAJTRC010000020        48311 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.020, whole genome shotgun sequence.
ACCESSION   DAJTRC010000020 DAJTRC010000000
VERSION     DAJTRC010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 48311)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48311)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48311
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.020"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            187..480
                     /locus_tag="OO357_001245"
     CDS             187..480
                     /locus_tag="OO357_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5886254.1"
                     /translation="MKPTTLLLIFTFFAMPGIVYAESPFSSLQSAKEKTTVLQDLRKI
                     CTPHASLSDEAWEKLMLSDENNKQHIREAIVAMERNNQSNYWEALGKVECPDM"
     gene            complement(521..1711)
                     /gene="nepI"
                     /locus_tag="OO357_001246"
     CDS             complement(521..1711)
                     /gene="nepI"
                     /locus_tag="OO357_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine ribonucleoside efflux pump NepI"
                     /protein_id="HCR5886255.1"
                     /translation="MSEFIAENRGADAITRPNWSAVFSVAFCVACLIIVEFLPVSLLT
                     PMAQDLGISEGVAGQSVTVTAFVAMFASLFITQTIQATDRRYVVILFAVLLTLSCLLV
                     SFANSFSLLLIGRACLGLALGGFWAMSASLTMRLVPPRTVPKALSVIFGAVSIALVIA
                     APLGSFLGELIGWRNVFNAAAVMGVLCIFWIIKSLPSLPGEPSHQKQNTFRLLQRPGV
                     MAGMIAIFMSFAGQFAFFTYIRPVYMNLAGFGVDGLTLVLLSFGIASFIGTSLSSFIL
                     KRSVKLALAGAPLILAVSALVLTLWGSDKIVATGVAIIWGLTFALVPVGWSTWITRSL
                     ADQAEKAGSIQVAVIQLANTCGAAIGGYALDNIGLTSPLMLSGTLMLLTALLVTAKVK
                     MKKS"
     gene            complement(1922..2374)
                     /locus_tag="OO357_001247"
     CDS             complement(1922..2374)
                     /locus_tag="OO357_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1198 domain-containing protein"
                     /protein_id="HCR5886256.1"
                     /translation="MIWIMLATLAVVFVVGFRVLTSGARKAIRRLSDRLNIDVVPVES
                     MVDQMGKSAGDEFLRYLHRPDESHLQNAAQVLLIWQIVIVDDSEQNLLQWHRILQKAR
                     LSAPITDAQVRLALGFLRETEPEMQDINAFQMRYNAFFQPAEGVHWLH"
     gene            complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="OO357_001248"
                     /gene_synonym="yicO"
     CDS             complement(2427..3761)
                     /gene="adeQ"
                     /locus_tag="OO357_001248"
                     /gene_synonym="yicO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418120.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeQ"
                     /protein_id="HCR5886257.1"
                     /translation="MNNDNTDYVSNESGTLSRLFKLPQHGTTVRTELIAGMTTFLTMV
                     YIVFVNPQILGAAQMDPKVVFVTTCLIAGIGSIAMGIFANLPVALAPAMGLNAFFAFV
                     VVGAMGISWQTGMGAIFWGAIGLFLLTLFRIRYWMISNIPLSLRIGITSGIGLFIALM
                     GLKNTGVIVANKDTLVMIGDLSSHGVLLGILGFFIITVLSSRHFHAAVLVSIVVTSCC
                     GLFFGDVHFSGVYSIPPDISGVIGEVDLSGALTLELAGIIFSFMLINLFDSSGTLIGV
                     TDKAGLIDSNGKFPNMNKALYVDSVSSVAGAFIGTSSVTAYIESTSGVAVGGRTGLTA
                     VVVGVMFLLVMFFSPLVAMVPPYATAGALIFVGVLMTSSLARVNWDDFTESVPAFITT
                     VMMPFTFSITEGIALGFMSYCIMKVCTGRWRDLNLCVVVVAALFALKIILVD"
     gene            3936..5702
                     /gene="adeD"
                     /locus_tag="OO357_001249"
     CDS             3936..5702
                     /gene="adeD"
                     /locus_tag="OO357_001249"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HCR5886258.1"
                     /translation="MNNSINHKFHHISRAEYQELLAVSRGDAVADYIIDNVSILDLIN
                     AGEISGPIVIKGRYIAGVGAEYADTPALQRIDARGATAVPGFIDAHLHIESSMMTPVT
                     FETATLPRGLTTVICDPHEIVNVMGEAGFAWFARCAEQARQNQYLQVSSCVPALEGCD
                     VNGASFTLEQMLAWRDHPQVTGLAEMMDYPGVINGQNALLDKLDAFRYLTLDGHCPGL
                     GGKELNAYIAAGIENCHESYQLEEGRRKLQLGMSLMIREGSAASNLNALAPLINEFNS
                     PQCMLCTDDRNPWEIAHEGHIDALIRRLIEQHNVPLHVAYRVASWSTARHFGLNHLGL
                     LAPGKQADIVLLSDARKVTVQQVLVKGEPIDAQTLQAEESARLALSAPPYGNTIARQP
                     VSASDFALQFTPGKRYRVIDVIHNELITHSRSSVYSENGFDRDDVCFIAVLERYGQRL
                     APACGLLGGFGLNEGALAATVSHDSHNIVVIGRSAEEMALAVNQVIQDGGGLCVVRNG
                     QVQSHLPLPIAGLMSTDTAQSLAEQIDALKAAARECGPLPDEPFIQMAFLSLPVIPAL
                     KLTSQGLFDGEKFAFTTLEVTE"
     gene            complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="OO357_001250"
     CDS             complement(5748..7139)
                     /gene="uhpT"
                     /locus_tag="OO357_001250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose-6-phosphate:phosphate antiporter"
                     /protein_id="HCR5886259.1"
                     /translation="MLAFLNQVRKPTLDLPLEVRRKMWFKPFMQSYLVVFIGYLTMYL
                     IRKNFNIAQNDMISTYGLSMTQLGMIGLGFSITYGVGKTLVSYYADGKNTKQFLPFML
                     ILSAICMLGFSASMGSGSVSLFLMIAFYALSGFFQSTGGSCSYSTITKWTPRRKRGTF
                     LGFWNISHNLGGAGAAGVALFGANYLFDGHVIGMFIFPSIIALIVGFIGLRYGSDSPE
                     SYGLGKAEELFGEEISEEDKETESTDMTKWQIFVEYVLKNKVIWLLCFANIFLYVVRI
                     GIDQWSTVYAFQELKLSKAVAIQGFTLFEAGALVGTLLWGWLSDLANGRRGLVACIAL
                     ALIIATLGVYQHASNEYIYLASLFALGFLVFGPQLLIGVAAVGFVPKKAIGAADGIKG
                     TFAYLIGDSFAKLGLGMIADGTPVFGLTGWAGTFAALDIAAIGCICLMAIVAVMEERK
                     IRREKKIQQLTVA"
     gene            complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="OO357_001251"
     CDS             complement(7277..8596)
                     /gene="uhpC"
                     /locus_tag="OO357_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709527.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter family glucose-6-phosphate
                     receptor UhpC"
                     /protein_id="HCR5886260.1"
                     /translation="MLPFLKAPADAPLMTDKHEIDARYRYWRRHILLTIWLGYALFYF
                     TRKSFNAAVPEILANGVLSRSDIGLLATLFYITYGVSKFVSGIVSDRSNARYFMGIGL
                     IATGIMNILFGFSTSLWAFAVLWVLNAFFQGWGSPVCARLLTAWYSRTERGGWWALWN
                     TAHNVGGALIPIVMAASALHYGWRAGMMIAGCMAIVVGIFLCWRLRDRPQALGLPAVG
                     EWRHDALEIAQQQEGAGLTRKEILTKYVLLNPYIWLLSFCYVLVYVVRAAINDWGNLY
                     MSETLGVDLVTANTAVTMFELGGFIGALVAGWGSDKLFNGNRGPMNLIFAAGILLSVG
                     SLWLMPFASYVMQATCFFTIGFFVFGPQMLIGMAAAECSHKEAAGAATGFVGLFAYLG
                     ASLAGWPLAKVLDTWHWSGFFVVISIAAGISALLLLPFLNAQTPREA"
     gene            complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="OO357_001252"
     CDS             complement(8606..10108)
                     /gene="uhpB"
                     /locus_tag="OO357_001252"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction histidine-protein
                     kinase/phosphatase UhpB"
                     /protein_id="HCR5886261.1"
                     /translation="MKTLFSRLITVIACFFIFSAAWFCLWSISLHLVERPDMAVLLFP
                     FGLRLGLMLQCPRGYWPVLLGAEWLLIYWLTQAVGLTHFPLLMIGSLLTLLPVALISR
                     YRHQRDWRTLLLQGAALTAAALLQSLPWLWHGKESWNALLLTLTGGLTLAPICLVFWH
                     YLANNTWLPLGPSLVSQPINWRGRHLVWYLLLFVISLWLQLGLPDELSRFTPFCLALP
                     IIALAWHYGWQGALIATLMNAIALIASQTWRDHLVDLLLSLLVQSLTGLLLGAGIQRL
                     RELNQSLQKELARNQHLAERLLETEESVRRDVARELHDDIGQTITAIRTQAGIVQRLA
                     ADNASVKQSGQLIEQLSLGVYDAVRRLLGRLRPRQLDDLTLEQAIRSLMREMELEGRG
                     IVSHLEWRIDESALSENQRVTLFRVCQEGLNNIVKHADASAVTLQGWQQDERLMLVIE
                     DDGSGLPPGSGQQGFGLTGMRERVTALGGTLHISCLHGTRVSVSLPQRYV"
     gene            complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="OO357_001253"
     CDS             complement(10108..10698)
                     /gene="uhpA"
                     /locus_tag="OO357_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator UhpA"
                     /protein_id="HCR5886262.1"
                     /translation="MITVALIDDHLIVRSGFAQLLGLEPDLQVVAEFGSGREALAGLP
                     GRGVQVCICDISMPDISGLELLSQLPKGMATIMLSVHDSPALVEQALNAGARGFLSKR
                     CSPDELIAAVHTVATGGCYLTPDIAIKLASGRQDPLTKRERQVAEKLAQGMAVKEIAA
                     ELGLSPKTVHVHRANLMEKLGVSNDVELARRMFDGW"
     gene            complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="OO357_001254"
     CDS             complement(10773..11063)
                     /gene="ilvN"
                     /locus_tag="OO357_001254"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5886263.1"
                     /translation="MQNTTHNNVILELTVRNHPGVMTHVCGLFARRAFNVEGILCLPI
                     QDSDKSHIWLLVNDDQRLEQMISQIAKLEDVVKVQRNQSDPTMFNKIAVFFQ"
     gene            complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="OO357_001255"
     CDS             complement(11067..12755)
                     /gene="ilvB"
                     /locus_tag="OO357_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase large subunit"
                     /protein_id="HCR5886264.1"
                     /translation="MASSGTTSTRKRFTGAEFIVHFLEQQGIKIVTGIPGGSILPVYD
                     ALSQSTQIRHILARHEQGAGFIAQGMARTDGKPAVCMACSGPGATNLVTAIADARLDS
                     IPLICITGQVPASMIGTDAFQEVDTYGISIPITKHNYLVRHIEELPQVMSDAFRIAQS
                     GRPSPVWIDIPKDVQTAVFEIEAQPAVAEKAVAPAFSEESIRDAATMINAAKRPVLYL
                     GGGVINAPARVRELAEKAQLPTTMTLMALGMLPKAHPLSLGMLGMHGVRSTNYILQEA
                     DLLIVLGARFDDRAIGKTEQFCPNAKIIHVDIDRAELGKIKQPHVAIQADVDDVLAQL
                     IPRVEAQPRAEWHQLVADLQREFPCPIPKACDPLSHYGLINAVAACVDDNAIITTDVG
                     QHQMWTAQAYPLNRPRQWLTSGGLGTMGFGLPAAIGAALANPDRKVLCFSGDGSLMMN
                     IQEMATASENQLDVKIILMNNEALGLVHQQQSLFYEQGVFAATYPGKINFMQIAAGFG
                     LETCDLNNEADPQAALQEIINRPGPALIHVRIDAEEKVYPMVPPGAANTEMVGE"
     gene            complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="OO357_001256"
     CDS             complement(12861..12959)
                     /gene="ivbL"
                     /locus_tag="OO357_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilvB operon leader peptide IvbL"
                     /protein_id="HCR5886265.1"
                     /translation="MTTSMFNAKLLPTAPSAAVVVVRVVVVVGNAP"
     gene            13524..13613
                     /gene="tisB"
                     /locus_tag="OO357_001257"
     CDS             13524..13613
                     /gene="tisB"
                     /locus_tag="OO357_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin TisB"
                     /protein_id="HCR5886266.1"
                     /translation="MSLVDIAILILKLIVAALQLLDAVLKYLK"
     gene            13893..15077
                     /gene="emrD"
                     /locus_tag="OO357_001258"
     CDS             13893..15077
                     /gene="emrD"
                     /locus_tag="OO357_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EmrD"
                     /protein_id="HCR5886267.1"
                     /translation="MKRQRNVNLLLMLVLLVAVGQMAQTIYIPAIADMARDLNVREGA
                     VQSVMGAYLLTYGVSQLFYGPISDRVGRRPVILVGMSIFMLATLVAVTTSSLTVLIAA
                     SAMQGMGTGVGGVMARTLPRDLYERTQLRHANSLLNMGILVSPLLAPLIGGLLDTMWN
                     WRACYLFLLVLCAGVTFSMARWMPETRPVDAPRTRLLTSYKTLFGNSGFNCYLLMLIG
                     GLAGIAAFEACSGVLMGAVLGLSSMTVSILFILPIPAAFFGAWFAGRPNKRFSTLMWQ
                     SVICCLLAGLLMWIPDWFGVMNVWTLLVPAALFFFGAGMLFPLATSGAMEPFPFLAGT
                     AGALVGGLQNIGSGVLASLSAMLPQTGQGSLGLLMTLMGLLIVLCWLPLATRMSHQGQ
                     PV"
     gene            complement(15085..15582)
                     /locus_tag="OO357_001259"
     CDS             complement(15085..15582)
                     /locus_tag="OO357_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HCR5886268.1"
                     /translation="MTGSQVIDAEEDRHKLVVEYKDALQPADFYHNFKQRGIRSVQLI
                     PYLEFDDRGDLTAASVTAELWGKFLIALFECWVRADISRISIELFDATLQKWCGSENP
                     QPRRDCQACDWHRLCPHARQETPDSVLCAGYQAFYSYSAPHMRVMRDLIKQHRSPMEL
                     MTMLR"
     gene            complement(15579..15941)
                     /locus_tag="OO357_001260"
     CDS             complement(15579..15941)
                     /locus_tag="OO357_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF202 domain-containing protein"
                     /protein_id="HCR5886269.1"
                     /translation="MPDSRKARRIADPGLQPERTSLAWFRTMLGYGALMALAIKHNWH
                     QAGMLFWISIGILAIVALILWHYTRNRNLMDVTNSDFSQFHVVRDKFLISLAVLSLAI
                     LFAVTHIHQLIVFIERVA"
     gene            complement(15931..16278)
                     /locus_tag="OO357_001261"
     CDS             complement(15931..16278)
                     /locus_tag="OO357_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidH family protein"
                     /protein_id="HCR5886270.1"
                     /translation="MKISRLGEAPDYRFSLANERTFLAWIRTALGFLAAGVGLDQLAP
                     DFATPVIRELLALLLCLFSGGLAMYGYLRWLRNEKAMRLKEDLPYTNSLLIISLILMV
                     VAVIVMGLVLYAG"
     gene            16387..16800
                     /locus_tag="OO357_001262"
     CDS             16387..16800
                     /locus_tag="OO357_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886271.1"
                     /translation="MGIIAQNKISSLGMLFGAIALMMGIIHFSFGPFSAPPPTLESIV
                     ADKTAEIKRGLLAGIKGEKITTVEKKEDMDIDKILDQSGIALAIAALLCAFIGGMRKE
                     NRWGIRGALVFGIGIVCSILLIFLIFSFLTGGSLV"
     gene            complement(16847..18289)
                     /locus_tag="OO357_001263"
                     /pseudo
     CDS             complement(16847..18289)
                     /locus_tag="OO357_001263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418134.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
     gene            complement(18324..20039)
                     /locus_tag="OO357_001264"
     CDS             complement(18324..20039)
                     /locus_tag="OO357_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="solute:sodium symporter family transporter"
                     /protein_id="HCR5886272.1"
                     /translation="MNSLQILSFVGFTLLVAVITWWKVRKTDTGSQQGYFLAGRSLKA
                     PVIAASLMLTNLSTEQLVGLSGQAYKSGMSVMGWEVTSAVTLIFLALIFLPRYLKRGI
                     ATIPDFLEERYDKTTRIIIDFCFLIATGVCFLPIVLYSGALALNSLFHVGESLQISHG
                     AAIWLLVILLGLAGILYAVIGGLRAMAVADSINGIGLVIGGLMVPVFGLIAIGKGSFM
                     QGIEQITTVHAEKLNSVGGPTDPLPIGAAFTGLILVNTFYWCTNQGIVQRTLASKSLA
                     EGQKGALLTAVLKMLDPLVLVLPGLIAFHLYQDLPKADMAYPTLVNNVLPVPLEGFFG
                     AVLFGAVISTFNGFLNSASTLFSMGIYRRIINQNAEPQQLVTVGRKFGFFIAIVSVLV
                     APWIANAPQGLYSWMKQLNGIYNVPLVTIIIMGFFFPRIPALAAKVAMGIGIISYITI
                     NYLVKFDFHFLYVLACTFCINVVVMLVIGFIKPRATPFTFKDAFAVDMKPWKNVKIAS
                     IGILFAMIGVYVGLAEFGGYGTRWLAMISYFIAAVVIVYLIFDSWWHRHDPAVTFTPD
                     AKDSL"
     gene            20206..21099
                     /locus_tag="OO357_001265"
     CDS             20206..21099
                     /locus_tag="OO357_001265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5886273.1"
                     /translation="MNGKLQSSDVKNETPYNIPLLINENVISSGISLISLWHTYADEH
                     YRVIWPRDKKKPLIANSWVAVYTVQGCGKILLKNGEQITLHSNCIIFLKPMDIHSYHC
                     EGLVWEQYWMEFTPTSMMDISVGQQSVIYNGEIYNQELTEVAELITSPEAIKNNLAVA
                     FLTKIIYQWICLMSADGKKDPQRRQIEKLIATLHASLQQRWSVADMAATIPCSEAWLR
                     RLFLRYTGKTPKEYYLDARLDLALSLLKQQGNSVGEVADTLNFFDSFHFSKAFKHKFG
                     YAPSAVLKNTDQHPTDASPHN"
     gene            complement(21096..21910)
                     /locus_tag="OO357_001266"
                     /pseudo
     CDS             complement(21096..21910)
                     /locus_tag="OO357_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312649.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(21910..23526)
                     /locus_tag="OO357_001267"
     CDS             complement(21910..23526)
                     /locus_tag="OO357_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HCR5886274.1"
                     /translation="MLSQIQRFGGAMFTPVLLFPFAGIVVGLAILLQNPMFVGESLTD
                     PNSLFAQIVHIIEEGGWTVFRNMPLIFAVGLPIGLAKQAQGRACLAVMVSFLTWNYFI
                     NAMGMTWGSYFGVDFTQDAVAGSGLTMMAGIKTLDTSIIGAIIISGIVTALHNRLFDK
                     KLPVFLGIFQGTSYVVIIAFLVMIPCAWLTLLGWPKVQMGIESLQAFLRSAGALGVWV
                     YTFLERILIPTGLHHFIYGPFIFGPAAVEGGIQMYWAQHLQEFSLSAEPLKSLFPEGG
                     FALHGNSKIFGAVGISLAMYFTAAPENRVKVAGLLIPATLTAMLVGITEPLEFTFLFI
                     SPLLFAVHAVLAASMSTVMYLFGVVGNMGGGLIDQVLPQNWIPMFSNHADMMLTQIAI
                     GLCFTLLYFVVFRTLILQFNMCTPGREDAEVKLYSKAEYKASRGQTTAAEPKKELDQA
                     AGILQALGGVGNISSINNCATRLRIALHDMSQTLDDEVFKKLGAHGVFRSGDAIQVII
                     GLHVSQLREQLDSLINSHQSAENVAITEAV"
     gene            23821..24495
                     /locus_tag="OO357_001268"
     CDS             23821..24495
                     /locus_tag="OO357_001268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5886275.1"
                     /translation="MIYKSIAERLRIRLNSADFTLNSLLPGEKKLAEEFAVSRMTIRK
                     AIDLLVAWGLVVRRHGSGTYLVRKDVLHQTASLTGLVEVLKRQGKTVTSQVLIFEIMP
                     APPAIASQLRIQINEQIYFSRRVRFVEGKPLMLEDSYMPVKLFRNLSLQHLEGSKFEY
                     IEQECGILIGGNYESLMPVLADRLLARQMKVAEHTPLLRITSLSYSESGEFLNYSVMF
                     RNASEY"
     gene            complement(24534..26195)
                     /locus_tag="OO357_001269"
     CDS             complement(24534..26195)
                     /locus_tag="OO357_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative transporter"
                     /protein_id="HCR5886276.1"
                     /translation="MSDIALTVSILALVAVVGLFIGNVKFRGIGLGIGGVLFGGIIVG
                     HFVSQAGMTLSSDMLHVIQEFGLILFVYTIGIQVGPGFFASLRVSGLRLNLFAVLIVI
                     IGGLVTAILHKLFDIPLPVVLGIFSGAVTNTPALGAGQQILRDLGTPMEMVDQMGMSY
                     AMAYPFGICGILFTMWMLRVIFRVNVETEAQQHESSRTNGGALIKTINIRVENPNLHD
                     LAIKDVPILNGDKIICSRLKREETLKVPSPDTIIQLGDLLHLVGQPADLHNAQLVIGQ
                     EVDTSLSTKGTDLRVERVVVTNENVLGKRIRDLHFKERYDVVISRLNRAGVELVASGD
                     ISLQFGDILNLVGRPSAIDAVANVLGNAQQKLQQVQMLPVFIGIGLGVLLGSIPVFVP
                     GFPAALKLGLAGGPLIMALILGRIGSIGKLYWFMPPSANLALRELGIVLFLSVVGLKS
                     GGDFVNTLVNGEGLSWIGYGALITAVPLITVGILARMLAKMNYLTMCGMLAGSMTDPP
                     ALAFANNLHPTSGAAALSYATVYPLVMFLRIITPQLLAVLFWSIG"
     gene            complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="OO357_001270"
     CDS             complement(26391..26819)
                     /gene="ibpB"
                     /locus_tag="OO357_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpB"
                     /protein_id="HCR5886277.1"
                     /translation="MRNFDLSPLMRQWIGFDKLANALQNAGESQSFPPYNIEKSDDNH
                     YRITLALAGFRQEDLEIQLEGTRLSVKGTPEQPKEEKKWLHQGLMNQPFSLSFTLAEN
                     MEVSGATFVNGLLHIDLIRNEPEPIAAQRIAISERPALNS"
     gene            complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="OO357_001271"
     CDS             complement(26931..27344)
                     /gene="ibpA"
                     /locus_tag="OO357_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006687722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock chaperone IbpA"
                     /protein_id="HCR5886278.1"
                     /translation="MRNFDLSPLYRSAIGFDRLFNHLENNQSQSNGGYPPYNVELVDE
                     NHYRIAIAVAGFAESELEITAQDNLLVVKGAHADEQKERTYLYQGIAERNFERKFQLA
                     ENIHVRGANLVNGLLYIDLERVIPEAKKPRRIEIN"
     misc_feature    complement(27338..27410)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01832"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ROSE (Repression Of Heat Shock gene Expression)
                     occurs in the 5'-region of heat shock genes and acts as an
                     RNA thermometer to modulate expression; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF01832"
     gene            27649..27981
                     /locus_tag="OO357_001272"
     CDS             27649..27981
                     /locus_tag="OO357_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceK/YidQ family lipoprotein"
                     /protein_id="HCR5886279.1"
                     /translation="MIRNVLLAFMICSGMTLLGGCSSVMSHTGGKEGTYPGTRASATM
                     IGDDETNWGTKSLAILDMPFTAVMDTLLLPWDVFRKDSSVRSRVEKSEANAQATNAVI
                     PPARMPDN"
     gene            complement(27983..29215)
                     /locus_tag="OO357_001273"
     CDS             complement(27983..29215)
                     /locus_tag="OO357_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3748 domain-containing protein"
                     /protein_id="HCR5886280.1"
                     /translation="MKQITFAPRNHLLTNTNTNTNTNTWTPDSQWLVFDVRPSGASFT
                     GETIERVNIHTGEVEVIYRASQGAHVGVVTVHPKSEKYVFIHGPENPDETWHYDFHHR
                     RGVIAEGGKVSNLDAMDITAPYTPGALRGGSHVHVFSPNGERGSFTYNDHVMHQLDPA
                     LDLRNVGVAAPFGPVNVQKQHPREYSGSHWCVLVSKTTPTPQPGSDEINRAYEEGWVG
                     NHALAFIGDTLSPKGEKVPELFIVELPQDEASWKSAGDAPLSGTETTLPAPPRGVVQR
                     RLTFTHHRAYPGLVNVPRHWVRCNPQGTQIAFLMRDDNGIVQLWLISPQGGEPRKLTH
                     NKTDIQSAFNWHPSGEWLGFVLDNRIVCAHAQSGEVEYLTENHANPPSADAVVFSPDG
                     QWLAWMEGGQLWITETDR"
     gene            29316..30416
                     /gene="cbrA"
                     /locus_tag="OO357_001274"
     CDS             29316..30416
                     /gene="cbrA"
                     /locus_tag="OO357_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colicin M resistance protein"
                     /protein_id="HCR5886281.1"
                     /translation="MEHFDVAIIGLGPAGSALARKLAGKMQVIALDKKHQCGTEGFSK
                     PCGGLLAPDAQRSFIRDGLTLPVDVIANPQIFSVKTVDVAASLTRNYQRSYININRHA
                     FDLWMKSLIPASVEVYHDSLCRKIWREDDKWHVIFRADGWEQHITARYLVGADGANSM
                     VRRHLYPDHQIRKYVAIQQWFAEKHPVPFYSCIFDNAITDCYSWSISKDGYFIFGGAY
                     PMKDGQTRFTTLKEKMSAFQFQFGKAVKSEKCTVLFPSRWQDFVCGKDNAFLIGEAAG
                     FISASSLEGISYALDSAEILRAVFLKQPEKSNAAYWRATHKLRLKLFGKIVKSRCLTA
                     PALRKWIMRSGVAHIPQLKDYPTRFTSPTSRM"
     gene            complement(30377..31714)
                     /locus_tag="OO357_001275"
     CDS             complement(30377..31714)
                     /locus_tag="OO357_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5886282.1"
                     /translation="MVSGFAMPKIWRKLAMDIPVNAAKPGRRRYLTLVMIFITVVICY
                     VDRANLAVASAHIQEEFGITKAEMGYVFSAFAWLYTLCQIPGGWFLDRVGSRVTYFIA
                     IFGWSVATLFQGFATGLMSLIGLRAITGIFEAPAFPTNNRMVTSWFPEHERASAVGFY
                     TSGQFVGLAFLTPLLIWIQEMLSWHWVFIVTGGIGIIWSLIWFKVYQPPRLTKGISKA
                     ELDYIRDGGGLVDGDAPVKKEARQPLTAKDWKLVFHRKLIGVYLGQFAVASALWFFLT
                     WFPNYLTQEKGITALKAGFMTTVPFLAAFVGVLLSGWVADLLVRKGFSLGFARKTPII
                     CGLLISTCIMGANYTNDPMMIMCLMALAFFGNGFASITWSLVSSLAPMRLIGLTGGVF
                     NFAGGLGGITVPLVVGYLAQGYGFAPALVYISAVALIGALSYILLVGDVKRVG"
     gene            complement(31789..32937)
                     /gene="dgoD"
                     /locus_tag="OO357_001276"
                     /pseudo
     CDS             complement(31789..32937)
                     /gene="dgoD"
                     /locus_tag="OO357_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077623.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactonate dehydratase"
     gene            complement(32934..33551)
                     /gene="dgoA"
                     /locus_tag="OO357_001277"
     CDS             complement(32934..33551)
                     /gene="dgoA"
                     /locus_tag="OO357_001277"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HCR5886283.1"
                     /translation="MQWQTKLPLIAILRGITPDEALAHVGAVIDAGFDAVEIPLNSPQ
                     WEQSIPAIVDAYGDKALIGAGTVLKPEQVDALARMGCQLIVTPNIHSEVIRRAVGYGM
                     TVCPGCATATEAFTALEAGAQALKIFPSSAFGPQYIKALKAVLPPDIAVFAVGGVTPE
                     NLAQWIDAGCVGAGLGSDLYRAGQSVERTAQQAAAFVKAYREAVQ"
     gene            complement(33535..34413)
                     /gene="dgoK"
                     /locus_tag="OO357_001278"
     CDS             complement(33535..34413)
                     /gene="dgoK"
                     /locus_tag="OO357_001278"
                     /EC_number="2.7.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HCR5886284.1"
                     /translation="MTARYIAIDWGSTNLRAWLYQGDHCLESRQSEAGVTRLNGKSPA
                     AVLAEVTTDWREENTPVVMAGMVGSNVGWKVAPYLSVPARFSSIGEQLTSVGDNIWII
                     PGLCVSHDDNHNVMRGEETQLIGARALAPSSLYVMPGTHCKWVQADSQQINDFRTVMT
                     GELHHLLLNHSLIGAGLPPQENSADAFAAGLERGLNAPAILPQLFEVRASHVLGTLPR
                     EQVSEFLSGLLIGAEVASMRDYVTHQHAITLVAGTSLTARYQQAFQAMGCDVAAVAGD
                     TAFQAGIRSIAHAVAN"
     gene            complement(34410..35099)
                     /gene="dgoR"
                     /locus_tag="OO357_001279"
     CDS             complement(34410..35099)
                     /gene="dgoR"
                     /locus_tag="OO357_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate utilization transcriptional
                     regulator DgoR"
                     /protein_id="HCR5886285.1"
                     /translation="MTLNKTDRIVITLGKQIVHGKYVPGSPLAAEAELCEEFATSRNI
                     IREVFRSLMAKRLIEMKRYRGAFVAPRNQWNYLDTDVLQWVLENDYDPRLISAMSEVR
                     NLVEPAIARWAAERATSSDLAQIESALNEMIANNQDREAFNEADIRYHEAVLQSVHNP
                     VLQQLSIAISSLQRAVFERTWMGDEANMPQTLQEHKALFDAIRHQDGDAAEQAALTMI
                     ASSTRRLKEIT"
     gene            35377..36033
                     /locus_tag="OO357_001280"
     CDS             35377..36033
                     /locus_tag="OO357_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886286.1"
                     /translation="MKLNFKGFFKAAGLFPLALMLSGCISYALVSHTAKGSSGKYQSQ
                     SDTITGLSQAKDSNGTKGYVFVGESLDYLITDGADDIVKMLNDPALNRHNIQVADDAR
                     FVLNAGKKKFTGTISLYYYWNNEEEKALATHYGFACGVQHCTRSLENLKGTIHEKNKN
                     MDYSKVMAFYHPFKVRFYEYYSPRGIPDGVSAALLPVTVTLDIITAPLQFLVVYAVNQ
                     "
     gene            complement(36079..36891)
                     /gene="yidA"
                     /locus_tag="OO357_001281"
     CDS             complement(36079..36891)
                     /gene="yidA"
                     /locus_tag="OO357_001281"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HCR5886287.1"
                     /translation="MAIKLIAIDMDGTLLLPDHTISPAVKNAIAAARARGVNVVLTTG
                     RPYAGVHNYLKELHMEQPGDYCITYNGALVQKAADGSTVAQTALSYDDYRFLEKLSRE
                     VGSHFHALDRTTLYTANRDISYYTVHESFVATIPLVFCEAEKMDPNTQFLKVMMIDEP
                     AILDQAIARIPQEVKEKYTVLKSAPYFLEILDKRVNKGTGVKSLADVLGIKPEEIMAI
                     GDQENDIAMIEYAGVGVAMDNAIPSVKEVANFVTKSNLEDGVAFAIEKYVLN"
     gene            complement(37005..37403)
                     /locus_tag="OO357_001282"
     CDS             complement(37005..37403)
                     /locus_tag="OO357_001282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HCR5886288.1"
                     /translation="MGLFDEVVGAFLKGDAGKYQAILSWVEEQGGIQVLLEKLQSGGL
                     GAILSTWLSNQQRNQSVSGEQLESALGTNAVSDLGQKLGVDTSTASSLLAEQLPKIID
                     ALSPQGEVSAQANNDLLSAGMELLKGKLFR"
     gene            complement(37643..40057)
                     /gene="gyrB"
                     /locus_tag="OO357_001283"
     CDS             complement(37643..40057)
                     /gene="gyrB"
                     /locus_tag="OO357_001283"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HCR5886289.1"
                     /translation="MSNSYDSSSIKVLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVV
                     DNAIDEALAGHCKEIIVTIHADNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGG
                     KFDDNSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYEHGVPQAPLAVTGET
                     EKTGTMVRFWPSLETFTNVTEFEYEILAKRLRELSFLNSGVSIRLRDKRDGKEDHFHY
                     EGGIKAFVEYLNKNKTPIHPNIFYFSTEKDGIGVEVALQWNDGFQENIYCFTNNIPQR
                     DGGTHLAGFRAAMTRTLNAYMDKEGYSKKAKVSATGDDAREGLIAVVSVKVPDPKFSS
                     QTKDKLVSSEVKSAVEQQMNELLAEYLLENPTDAKIVVGKIIDAARAREAARRAREMT
                     RRKGALDLAGLPGKLADCQERDPALSELYLVEGDSAGGSAKQGRNRKNQAILPLKGKI
                     LNVEKARFDKMLSSQEVATLITALGCGIGRDEYNPDKLRYHSIIIMTDADVDGSHIRT
                     LLLTFFYRQMPEIVERGHVYIAQPPLYKVKKGKQEQYIKDDEAMDQYQISIALDGATL
                     HTNASAPALAGEALEKLVSEYNATQKMINRMERRYPKAMLKELIYQPTLTEADLSDEQ
                     TVTRWVNALVSELNDKEQHGSQWKFDVHTNAEQNLFEPIVRVRTHGVDTDYPLDHEFI
                     TGGEYRRICTLGEKLRGLLEEDAFIERGERRQPVASFEQALDWLVKESRRGLSIQRYK
                     GLGEMNPEQLWETTMDPESRRMLRVTVKDAIAADQLFTTLMGDAVEPRRAFIEENALK
                     AANIDI"
     gene            complement(40086..41159)
                     /gene="recF"
                     /locus_tag="OO357_001284"
     CDS             complement(40086..41159)
                     /gene="recF"
                     /locus_tag="OO357_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HCR5886290.1"
                     /translation="MSLTRLLIRDFRNIETADLALSPGFNFLVGANGSGKTSVLEAIY
                     TLGHGRAFRSLQIGRVIRHEQEAFVLHGRLQGEERETAIGLTKDKQGDSKVRIDGTDG
                     HKVAELAHLMPMQLITPEGFTLLNGGPKYRRAFLDWGCFHNEPGFFTAWSNLKRLLKQ
                     RNAALRQVTRYEQLRPWDKELIPLAEQISTWRAEYSAGIAADMDDTCKQFLPEFSLTF
                     SFQRGWEKETEYAEVLERNFERDRQLTYTAHGPHKADLRIRADGAPVEDTLSRGQLKL
                     LMCALRLAQGEFLTRESGRRCLYLIDDFASELDDERRGLLASRLKATQSQVFVSAISA
                     EHVIDMSDENSKMFTVEKGKITD"
     gene            complement(41159..42259)
                     /gene="dnaN"
                     /locus_tag="OO357_001285"
     CDS             complement(41159..42259)
                     /gene="dnaN"
                     /locus_tag="OO357_001285"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006177590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HCR5886291.1"
                     /translation="MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSL
                     TGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSG
                     RSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLF
                     ETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNLLRVQI
                     GSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNE
                     KFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCE
                     NVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL"
     gene            complement(42264..43667)
                     /gene="dnaA"
                     /locus_tag="OO357_001286"
     CDS             complement(42264..43667)
                     /gene="dnaA"
                     /locus_tag="OO357_001286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HCR5886292.1"
                     /translation="MSLSLWQQCLARLQDELPATEFSMWIRPLQAELSDNTLALYAPN
                     RFVLDWVRDKYLNNINGLLTSFCGADAPQLRFEVGTKPVTQTPQAAVTSNVAAPAQVA
                     QTQPQRAAPSTRSGWDNVPAPAEPTYRSNVNVKHTFDNFVEGKSNQLARAAACQVADN
                     PGGAYNPLFLYGGTGLGKTHLLHAVGNGIMARKPNAKVVYMHSERFVQDMVKALQNNA
                     IEEFKRYYRSVDALLIDDIQFFANKERSQEEFFHTFNALLEGNQQIILTSDRYPKEIN
                     GVEDRLKSRFGWGLTVAIEPPELETRVAILMKKADENDIRLPGEVAFFIAKRLRSNVR
                     ELEGALNRVIANANFTGRAITIDFVREALRDLLALQEKLVTIDNIQKTVAEYYKIKVA
                     DLLSKRRSRSVARPRQMAMALAKELTNHSLPEIGDAFGGRDHTTVLHACRKIEQLREE
                     SHDIKEDFSNLIRTLSS"
     gene            43964..44044
                     /locus_tag="OO357_001287"
     CDS             43964..44044
                     /locus_tag="OO357_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886293.1"
                     /translation="MTIDKNWLNRSNKDPGRSLRFTHQPV"
     gene            44274..44414
                     /gene="rpmH"
                     /locus_tag="OO357_001288"
     CDS             44274..44414
                     /gene="rpmH"
                     /locus_tag="OO357_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HCR5886294.1"
                     /translation="MKRTFQPSVLKRNRSHGFRARMATKNGRQVLARRRAKGRARLTV
                     SK"
     gene            44431..44790
                     /gene="rnpA"
                     /locus_tag="OO357_001289"
     CDS             44431..44790
                     /gene="rnpA"
                     /locus_tag="OO357_001289"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014172301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HCR5886295.1"
                     /translation="MVKLAFPRELRLLTPSQFTFVFQQPQRAGTPQITILGRLNSLGH
                     PRIGLTVAKKNVRRAHERNRIKRLTRESFRLRQHELPAMDFVVVAKKGVADLDNRALS
                     EALEKLWRRHCRLARGS"
     gene            44754..45011
                     /gene="yidD"
                     /locus_tag="OO357_001290"
     CDS             44754..45011
                     /gene="yidD"
                     /locus_tag="OO357_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HCR5886296.1"
                     /translation="MAPPLSPGSRVLIALIRVYQRLISPLLGPHCRFTPTCSSYGIEA
                     LRRFGVIKGSWLTVKRVLKCHPLHPGGDDPVPPGPFDTREH"
     gene            45014..46660
                     /gene="yidC"
                     /locus_tag="OO357_001291"
     CDS             45014..46660
                     /gene="yidC"
                     /locus_tag="OO357_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709495.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HCR5886297.1"
                     /translation="MDSQRNLLVIALLFVSFMIWQAWEQDKNPQPQAQQTTQTTTTAA
                     GSAADQGVPASGQGKLISVKTDVLDLTINTRGGDVEQALLPAYPKELNSTQPFQLLET
                     SPQFIYQAQSGLTGRDGPDNPANGPRPLYNVEKDAYVLAEGQNELQVPMTYTDAAGNT
                     FTKTFVLKRGDYAVNVNYNVQNAGEKPLEISSFGQLKQSITLPPHLDTGSSNFALHTF
                     RGAAYSTPDEKYEKYKFDTIADNENLNISSKGGWVAMLQQYFATAWIPHNDGTNNFYT
                     ANLGNGIVAIGYKSQPVLVQPGQTGAMNSTLWVGPEIQDKMAAVAPHLDLTVDYGWLW
                     FISQPLFKLLKWIHSFVGNWGFSIIIITFIVRGIMYPLTKAQYTSMAKMRMLQPKIQA
                     MRERLGDDKQRISQEMMALYKAEKVNPLGGCFPLLIQMPIFLALYYMLMGSVELRQAP
                     FALWIHDLSAQDPYYILPILMGVTMFFIQKMSPTTVTDPMQQKIMTFMPVIFTVFFLW
                     FPSGLVLYYIVSNLVTIIQQQLIYRGLEKRGLHSREKKKS"
     gene            46766..48130
                     /gene="mnmE"
                     /locus_tag="OO357_001292"
     CDS             46766..48130
                     /gene="mnmE"
                     /locus_tag="OO357_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HCR5886298.1"
                     /translation="MSDNDTIVAQATPPGRGGVGILRISGFKAREVAETVLGKLPKPR
                     YADYLPFKDADGSVLDQGIALWFPGPNSFTGEDVLELQGHGGPVILDLLLKRILTIPG
                     LRIARPGEFSERAFLNDKLDLAQAEAIADLIDASSEQAARSALNSLQGAFSARVNHLV
                     EALTHLRIYVEAAIDFPDEEIDFLSDGKIEAQLNDVMADLDAVRAEARQGSLLREGMK
                     VVIAGRPNAGKSSLLNALAGREAAIVTDIAGTTRDVLREHIHIDGMPLHIIDTAGLRE
                     ASDEVERIGIERAWQEIEQADRVLFMVDGTTTDAVDPAEIWPEFIARLPAKLPITVVR
                     NKADITGETLGMSEVNGHALIRLSARTGEGVDVLRNHLKQSMGFDTNMEGGFLARRRH
                     LQALEQAAEHLQQGKAQLLGAWAGELLAEELRLAQQNLSEITGEFTSDDLLGRIFSSF
                     CIGK"
BASE COUNT        11973 a        13064 c        12218 g        11056 t
ORIGIN      
        1 gcggtaaggt cgctgcggtt taaaaaacga aacgctatcg ataagaataa aaaggaataa
       61 aagtggaata taaagtctgg cacttcttac tgaccacgca agcgcgtttc gtacagcatg
      121 atgagagcga tgaaagcaaa ctacacttat gcttcattcg atacacattt gtaaaaggtt
      181 gaatccatga aaccaacgac actactgctt attttcactt ttttcgctat gccaggcatc
      241 gtatacgccg agtcgccttt tagctcactg cagtccgcaa aagagaaaac cacggtatta
      301 caagatttac gtaaaatttg cacgccacat gcgtcattat cagatgaagc gtgggaaaag
      361 ttaatgttgt cagatgaaaa caataaacaa catatacgcg aggccatcgt ggcgatggaa
      421 cgtaataatc agagcaatta ttgggaagcg ttgggtaagg tagaatgccc ggatatgtaa
      481 gaacagcaac aactactgac caacgcttct ttacctgata tcaggatttc ttcattttca
      541 cctttgcagt aaccaacaat gcagtcagca acatcaatgt gccggacaac atcaacggcg
      601 aagtcagacc aatattatcc agcgcataac cgccgattgc cgcgccacag gtattagcaa
      661 gctgaataac cgccacctga atagacccgg ctttttctgc ctgatcggcc agcgagcggg
      721 tgatccacgt tgaccagccg acgggaacca atgcaaaagt tagcccccag ataatcgcca
      781 cgccggtagc aacgatttta tcgcttcccc acaacgtcag taccaacgca ctcacagcca
      841 gtattaacgg cgcgcctgct aaggccagtt ttaccgaacg tttaagaatg aacgacgaaa
      901 gcgacgtacc aataaagctg gcgataccaa aactcaacag caccagcgtt aagccatcca
      961 cgccgaatcc cgccaggttc atatacaccg ggcgaatata cgtgaagaaa gcaaactgcc
     1021 cggcgaaaga catgaagatg gcgatcatcc ctgccatcac acccggacgt tgtaataagc
     1081 ggaaagtatt ttgtttctga tgcgagggtt cgcctggcag tgaaggcaat gatttgataa
     1141 tccagaaaat acacagcacg cccatcaccg ccgccgcatt aaagacattg cgccaaccga
     1201 taagctcgcc taaaaaactg cccaacggcg cggcaatcac cagcgcaata gaaaccgcgc
     1261 cgaagatcac cgacagcgcc ttcggcaccg tacgcggcgg taccagacgc atggtcagcg
     1321 acgccgacat cgcccagaac ccgcccagcg ccagccccag acaggcacga ccgattaaaa
     1381 gcaaactgaa tgagttagca aaggaaacca gcaagcagga gagcgtcagc aaaacggcaa
     1441 acaaaataac aacgtagcgg cggtcagtgg cctgaattgt ctgggtaata aacaaactgg
     1501 caaacattgc cacaaaggcg gtcacggtca ccgattgccc ggcaacccct tccgaaatgc
     1561 ctaaatcctg ggccattggc gtcaacaaac tgacgggcaa aaactcaacg ataatcagac
     1621 aggcgacaca aaacgccacc gagaaaacgg ctgaccagtt cggtcgggtg atggcatccg
     1681 cgccgcggtt ttcggcaata aattcactca tggtgttacc cgtggcatgt ttcaggggaa
     1741 aagccgacca ctttaacatt gaaatgtgtg acgaatttaa cgtttttgca acttttacgg
     1801 tgttgcttga gctggagtta agatcgaaac ggaacagggc aaagattacc ggatgcggcg
     1861 tgaacgtctt atccacccga caggtcaggc gcggtctgta ggctgataag acgaaacggt
     1921 ttcaatgcaa ccagtgaacg ccctcggcag gctgaaagaa cgcgttatag cgcatctgaa
     1981 aagcattaat atcctgcatt tcaggttcgg tttcacgcag aaaacccagc gccagcctga
     2041 cctgagcatc ggtaatcggc gcggaaaggc gggctttttg taaaatccgg tgccattgca
     2101 gcaggttctg ttcgctatca tcgacaatga caatttgcca gataagcaac acctgcgcgg
     2161 cgttttgcag gtgcgactca tccgggcgat gcaaataacg taaaaattcg tcaccggctg
     2221 actttcccat ttgatcgacc atcgactcca cgggtacgac atcgatattc agccgatcgc
     2281 tgagacggcg aatcgctttt ctggccccgg atgtcagcac ccgaaaacca accacaaaca
     2341 ctaccgccag cgtggcgagc attatccaga tcatgagttc tcctgcgtaa tcagaactca
     2401 tgatatctgg aaaccctcgc cagttactaa tccaccagaa taatcttcaa tgcgaacaga
     2461 gctgcgacca ccaccacaca caggttcaga tcgcgccagc gcccggtgca cactttcatg
     2521 atgcagtacg acataaagcc gagtgcaatc ccttcggtga tcgagaaagt aaagggcatc
     2581 atcaccgtgg taataaacgc aggcaccgat tcggtaaaat catcccagtt aacgcgcgcc
     2641 aggctcgaag tcatcagcac gccaacaaag attaacgctc cagcggttgc gtaaggagga
     2701 accatcgcca ccagcggtga gaagaacata actaacagga acataacgcc gaccacgaca
     2761 gccgttagcc ccgtgcgacc accgactgcc acaccagaag tactttcaat ataggcagta
     2821 acagacgagg taccgataaa tgcgcctgcc accgaactga cgctatcaac atacagcgcc
     2881 ttattcatat tggggaattt accgttgcta tctattaagc ccgctttatc agttacacca
     2941 attaatgttc ctgatgaatc aaataggttg atcagcataa aggagaaaat gataccggcg
     3001 agttcaagtg ttaacgcgcc gctcaaatct acttcaccaa tgacgccgct aatatcaggc
     3061 ggaatggaat agacgccgct aaaatgaaca tcaccgaaaa ataatccaca gcaagacgtc
     3121 accacaatag aaaccagcac cgcggcatga aaatgacgtg atgacaacac ggttataata
     3181 aaaaacccta aaatacctaa caacacgccg tgagaactta aatcgccaat catcaccagc
     3241 gtgtctttat tggcgacaat aacaccagtg ttttttaatc ccattaaggc aataaataat
     3301 ccgattccac tggtgatacc aatacgtaaa cttaatggaa tgttggagat catccagtac
     3361 cggatacgaa acagcgtgag caaaaatagc ccaattgcgc cccagaatat cgcgcccatc
     3421 ccggtctgcc aggagatccc catcgccccc acgaccacga acgcaaagaa ggcgttcagc
     3481 cccattgccg gagccagcgc cacgggtaag ttagcaaata tccccatcgc aatactgccg
     3541 ataccggcaa tcaaacaggt ggtaacaaac accactttcg ggtccatttg tgccgcgccg
     3601 aggatttgcg ggttcacaaa aacaatgtac accatggtta aaaaagtggt catccccgca
     3661 atcaattctg tgcggacggt ggtcccatgc tgaggtagtt taaataatcg cgaaagcgtc
     3721 cctgattcat tactcacgta atcggtattg tcattattca tttttttgtc cattggagga
     3781 gatttaatcc cttgcttttg taaaacaaat gacaacaacg gttcagtgat aattatcaca
     3841 tttcaattgc acattaatag atattcttta ataatctcgc gacgtttctt tatgataaat
     3901 aataatcaaa ttgataaaat caaaatgaga aaaatatgaa taattccatt aaccataaat
     3961 ttcatcacat tagccgggct gaataccagg aattgttagc cgtttcccgt ggcgacgctg
     4021 ttgccgatta tattattgat aatgtctcta ttctcgacct gatcaatgcc ggagaaattt
     4081 ccggcccaat tgtgattaaa ggacgttaca ttgccggtgt tggcgcagaa tacgctgata
     4141 ctccggcttt gcagcggatt gatgctcgcg gcgcaacggc ggtgccaggg tttattgatg
     4201 ctcacctgca tattgaatcc agcatgatga cgccggtcac ttttgaaacc gctaccctgc
     4261 cgcgcggcct gacgaccgtt atttgcgacc ctcatgaaat cgtcaacgtg atgggagaag
     4321 ccggattcgc ctggtttgcc cgctgtgccg aacaggcaag gcaaaaccag tacttacagg
     4381 tcagctcttg cgtgcccgct ctggaaggct gcgatgttaa cggtgccagt tttacccttg
     4441 aacagatgct cgcctggcgg gaccatccgc aggttaccgg ccttgcagaa atgatggact
     4501 accctggcgt aattaacggg cagaatgcgc tgctcgataa actcgatgca tttcgctacc
     4561 tgacgctgga cggtcactgt ccgggtttgg gtggcaaaga acttaacgcc tatattgctg
     4621 cgggtattga aaactgccac gaaagttatc agctggaaga aggacgccgg aaattacaac
     4681 tcggcatgtc gttgatgatc cgcgaagggt ccgctgccag caatctcaac gcactggcac
     4741 cgttgatcaa cgaatttaac agcccgcaat gcatgctctg taccgatgac cgtaacccgt
     4801 gggagatcgc ccatgaaggg catatcgatg ccttaattcg ccgcctgatc gaacaacaca
     4861 atgtgccgct gcatgtggca tatcgcgtcg ccagctggtc gacggcgcgc cactttggtc
     4921 tgaatcacct cggcttactg gcacccggca agcaggccga tatcgtcctg ttgagcgatg
     4981 cgcgtaaggt cacggtgcag caggtactgg tgaaaggcga accgattgat gcgcaaacct
     5041 tacaggcgga agagtcggcg agactggcgc tgtctgcccc gccatatggc aataccattg
     5101 cccgccagcc agtttccgcc agcgactttg ccctgcaatt cacgcccgga aaacgctatc
     5161 gggtcattga cgtcatccat aacgaattga ttacgcactc ccgctccagc gtctacagcg
     5221 aaaatggttt tgatcgcgat gatgtgtgct ttattgccgt acttgagcgt tacgggcaac
     5281 ggctggctcc ggcctgtggt ttgctcggcg gctttggcct gaatgaaggc gcgctggctg
     5341 cgacagtcag ccatgacagc cataatattg tggtgatcgg tcgcagtgcc gaagagatgg
     5401 cgctggcggt caatcaggtg attcaggatg gcggcgggct gtgcgtggta cgtaacggcc
     5461 aagtacaaag tcatctgccg ttacccattg ccgggctgat gagcaccgac acggcgcagt
     5521 cgctggcgga acaaattgac gccttgaaag ccgccgcccg tgaatgcggt ccgttacccg
     5581 atgagccgtt tattcagatg gcgtttcttt ctctgccagt gatccccgcg ctaaaactaa
     5641 ccagtcaggg gctatttgat ggcgagaagt ttgccttcac tacgctggaa gtcacggaat
     5701 aataaaaaaa gcccggcgtc atgccgggca aaagtcacca gttacgttta tgccactgtc
     5761 aactgctgaa tttttttctc gcggcggatt ttgcgttctt ccattaccgc cactatcgcc
     5821 atcaggcaga tacaaccaat cgcggcgata tccagcgcgg cgaaggtgcc tgcccagccg
     5881 gtaaggccga ataccggcgt cccatcggca atcattccca gacctaactt ggcaaagctg
     5941 tcaccaatca ggtaagcaaa ggtgccttta ataccatcgg cagcgccaat cgctttttta
     6001 ggtacaaagc caacagcagc cacaccaatc aacaattgcg ggccaaagac caggaaaccc
     6061 aacgcaaaga gagaagccag atagatatat tcgttactgg catgttgata cacaccgagc
     6121 gtggcgataa tcagcgccag cgcgatgcag gccaccaggc cacggcgacc gttcgccagg
     6181 tcagagagcc agccccacag cagcgtaccg accagcgcac cagcttcaaa cagcgtaaag
     6241 ccctgaatcg ccaccgcttt agagagtttc agttcctgga acgcgtatac ggttgaccac
     6301 tggtcgatac caatacgtac cacatagagg aaaatgttgg cgaagcacag cagccagatc
     6361 actttgtttt tcagcacata ctcaacaaag atctgccact tggtcatatc ggtagattct
     6421 gtctctttgt cctcttcgct gatctcctcg ccgaacagtt cttcagcttt gccgaggcca
     6481 taagattccg gggagtcgct gccgtaacgc aggccgataa aaccgacaat cagcgcgata
     6541 atcgacggga agataaacat gccgatgaca tggccatcga acaggtaatt tgccccgaac
     6601 agcgccacac ctgctgcgcc tgcaccgcca aggttgtgag aaatattcca gaaaccgagg
     6661 aatgtcccgc gtttacgacg cggcgtccat ttggtgatgg tggagtaact gcacgaaccg
     6721 ccggtactct ggaaaaagcc gcttaaggcg tagaaggcaa tcatcaggaa caggctaacc
     6781 gagccgctgc ccatactggc actgaagccc agcatacaaa tggcagagag gataagcatg
     6841 aacggcagga attgtttggt gtttttgccg tcggcgtagt atgaaaccaa cgttttaccc
     6901 acgccataag tgatggagaa acccaggccg atcatcccca gctgcgtcat gctcaacccg
     6961 taggtcgaaa tcatatcgtt ctgcgcgatg ttaaagttct tgcgaatcag gtacatcgtc
     7021 aggtagccga taaagaccac caggtaggat tgcatgaacg gtttgaacca cattttgcgc
     7081 cgcacttcga gcggaaggtc cagggtcggc ttgcgaactt ggtttaggaa agccagcatg
     7141 ggttactcct gaaatgaata cctgcgtgag gcatgcattg taaaaatcag cagcaaatca
     7201 ggcctgagat agcgtccaag aaaaacctgg aaaatttctt agttttgccg gatatgaagt
     7261 gaaaaggtga gatgcatcac gcttcacgcg gtgtctgggc gttcaaaaag ggcagtaaca
     7321 gcagtgcgga aatcccggcg gcgatagaga taaccacaaa aaatccgctc cagtgccagg
     7381 tatcgagcac tttcgccagc ggccaaccag caagcgacgc ccccagataa gcaaacaagc
     7441 cgacaaaccc cgtcgccgcc cctgccgcct ctttgtggga acactctgcc gccgccatac
     7501 caattaacat ctgcgggcca aagacaaaaa aaccaatggt gaagaagcag gttgcctgca
     7561 tcacgtagct ggcaaatggc atcagccaca gcgagccgac tgaaagcaaa attccggcgg
     7621 cgaaaatcaa attcatcggc ccacggttgc cgttaaacaa tttgtccgag ccccaaccgg
     7681 ctaccagcgc accgataaat ccgcccagtt caaacatcgt cactgccgta ttcgccgtga
     7741 ccagatcgac gcccagcgtc tcggacatat acaaattgcc ccagtcgttg atcgccgccc
     7801 ggaccacata gaccagcaca tagcaaaacg aaagcagcca gatatacgga ttcagcaaca
     7861 catatttggt gaggatctct ttacgcgtca accctgcccc ttcttgttgt tgagcaattt
     7921 ccagcgcgtc gtgccgccat tcaccgaccg ccggtaaacc taacgcctgc gggcgatcgc
     7981 gtagccgcca gcagagaaaa atccccacga ctatcgccat acaaccagca atcatcatcc
     8041 cggcacgcca gccgtaatgc agcgcgctcg ctgccatcac aatgggaatg agtgcgccgc
     8101 cgacgttatg cgccgtgttc cataatgccc accaaccgcc gcgctcggta cgtgaatacc
     8161 aggccgttaa cagacgcgca cacaccggtg aaccccagcc ctggaaaaag gcgttcagca
     8221 cccagagcac ggcaaacgcc catagcgacg tcgagaagcc aaacagaatg ttcatgatgc
     8281 ccgtggcgat aagccctatc cccataaaat aacgggcatt tgagcgatcg ctgacaatgc
     8341 cggagacaaa cttcgacacg ccgtaagtaa tgtaaaacag ggtcgctaac aggccgatat
     8401 cgctacggct gagcacgccg ttagcaagga tttctggtac cgcggcgtta aaacttttcc
     8461 gcgtgaagta aaacagcgcg taacccagcc agatggtcag cagaatatgc cgacgccagt
     8521 agcgataacg ggcatcaatt tcatgtttat cagtcattaa tggcgcatcg gcaggcgctt
     8581 tcagaaacgg caacatcatc gaaccttaga cataacgttg aggtagagaa acgctgacac
     8641 gcgtgccgtg cagacaggaa atgtgtaatg tgccgcccag cgccgttacg cgctcgcgca
     8701 ttccggtgag gccaaaacct tgttgcccgg aacccggcgg caaaccgctg ccatcgtctt
     8761 caatcaccag catcaaccgt tcatcctgct gccagccttg cagcgtaacc gcgctggcat
     8821 cggcatgttt cacaatgttg ttcagccctt cctggcagac acgaaacagc gtcacgcgct
     8881 ggttttcgct taatgctgat tcatcgatac gccattcgag atggctgaca atgccgcgcc
     8941 cttccagctc catttcccgc atcagtgagc ggatggcctg ctccagggtg agatcatcca
     9001 gctggcgcgg acgtaaccgt cctaacaaac ggcgcaccgc gtcgtaaacg cccagcgaca
     9061 gttgttcgat gagctgcccg ctctgcttca cactggcgtt atctgccgcc agccgctgaa
     9121 caatgcccgc ctgagtacga atagcagtga tggtctgacc gatatcgtcg tgcagctcac
     9181 gcgccacgtc gcggcgcacg ctctcttcgg tttctaacaa acgttcagcc agatgctgat
     9241 tgcgcgccag ttccttttgt agcgactggt taagttcacg caaccgctgg atgcctgcgc
     9301 ccagtaacaa ccctgtcaga ctttgcacca gcaacgagag taataaatcc accagatgat
     9361 cgcgccaggt ttgactggcg atcagcgcga tggcgttcat caacgtcgca atcagcgctc
     9421 cttgccagcc atagtgccag gccagcgcga taatcggcag cgccagacag aatggcgtaa
     9481 agcgcgacag ttcgtccggc aatcccaact ggagccagag actgataaca aacagcagca
     9541 agtaccagac cagatgccgc ccgcgccagt tgatgggctg agaaaccagt gacgggccga
     9601 gcggcagcca ggtgttattg gcgagatagt gccagaacac cagacatatc ggggccagcg
     9661 tcagaccgcc agttaaagtc agcaacagcg cattccacga ctctttgccg tgccaaagcc
     9721 agggcagcga ctgcaacaac gccgccgccg ttaacgccgc accctgtaac agcaaggtgc
     9781 gccagtcacg ctgatggcga tagcgcgaga tcagcgctac gggcagtaac gtcagcaaac
     9841 taccgatcat caataacgga aaatgggtta aaccgaccgc ctgcgttagc cagtaaatca
     9901 gcagccactc cgcacccagc aatacgggcc agtatccgcg cgggcattgc agcattagcc
     9961 ccagacgcag accaaacgga aataacagca ccgccatatc agggcgctca accagatgca
    10021 ggctgatact ccataggcaa aaccatgcgg cagagaagat aaaaaagcag gcaataacgg
    10081 taattaagcg ggagaacaac gtcttcatca ccagccatca aacatgcggc gcgccagctc
    10141 tacgtcgtta ctgacgccca gtttttccat cagattggcg cgatggacgt gtaccgtttt
    10201 cggtgacaag cccagttcgg cggcaatctc tttcaccgcc attccttgcg ccagtttttc
    10261 cgccacctgg cgttcacgtt tggtcagcgg gtcctgacga ccggatgcca gtttaatggc
    10321 aatatccggc gtcagataac agccgcccgt ggcaaccgta tgcaccgcag caatgagttc
    10381 atccgggcta cagcgtttgg aaagaaagcc gcgtgccccc gcgttaagcg cctgctcaac
    10441 cagcgcagga ctgtcgtgaa cggagagcat aatcgtcgcc atacctttcg gcagctggct
    10501 tagcagctcc agaccggaga tatcgggcat ggagatatcg caaatacaca cctgcacacc
    10561 gcgccccggc agccccgcca gcgcctcgcg ccccgaacca aactcggcaa ctacctgcaa
    10621 atcaggttcc agccccagca gctgcgcaaa gccggagcgg acgatgaggt gatcgtctat
    10681 aagggcaacg gtgatcatgg tcttgtcctg gcgggtaaaa aaaaacgcgc ttaccttaac
    10741 gataagcgcg atgttgttca agccttgagc ggttactgaa aaaacaccgc gatcttgtta
    10801 aacatcgtcg gatcggactg attacgctgc actttcacga catcttccag cttagcgatt
    10861 tggcttatca tctgctccag acgctggtcg tcattgacca gtagccagat atggcttttg
    10921 tcgctgtcct gaatcggcag acaaagaatg ccttcaacgt taaaagcgcg gcgggcaaaa
    10981 aggccacaaa cgtgggtcat tacgcccgga tggttgcgaa cggtgagctc cagaattacg
    11041 ttgttatgag ttgtgttttg catggcttat tcccccacca tttcagtatt cgccgcacct
    11101 ggcggcacca tcgggtaaac tttttcttcg gcatcaatgc gcacatggat cagcgccggg
    11161 ccagggcgat tgatgatttc ctgcaatgca gcctgcggat cggcttcgtt attcaaatca
    11221 caggtttcga ggccgaatcc ggcggcaatc tgcataaagt tgattttgcc cggataggtg
    11281 gcggcaaaaa cgccttgctc gtagaacaga ctctgttgct gatgcaccag ccccagcgct
    11341 tcgttgttca tcagaatgat tttgacatcc agctgatttt cactggcggt cgccatctcc
    11401 tgaatattca tcatcaggct gccgtcgccg gagaaacaca acactttgcg atccgggttc
    11461 gccagcgccg cgccaatcgc cgcaggcagg ccaaaaccca tcgtgcccag cccaccggag
    11521 gtcagccact ggcgtgggcg attgagcgga taagcttgcg cggtccacat ctgatgctga
    11581 cccacatcgg tggtgataat cgcattgtca tcgacacagg cggcaacggc gttgatcagg
    11641 ccgtaatggc ttaatggatc gcacgctttc gggattggac acgggaattc gcgctgcaaa
    11701 tccgctacca actggtgcca ctctgcacgc ggttgcgctt ccacccgcgg gatcaattgc
    11761 gccagcacgt catcaacatc cgcctgaatc gccacatgcg gctgcttgat tttacccagc
    11821 tctgcgcgat cgatatcgac atgaataatt ttggcattcg gacagaactg ctcggttttg
    11881 ccaatcgccc ggtcatcaaa acgcgcaccg agcacaatca acaaatccgc ctcctgcaaa
    11941 atatagttgg tactgcgcac gccgtgcatc cccagcatac ccagcgacaa cggatgcgct
    12001 tttggcagca tgcccagcgc cattaaagtc atggtggtag gcagttgcgc tttctccgcc
    12061 agttcacgca cccgcgcagg cgcattgatc acaccaccac ccagataaag caccgggcgt
    12121 ttggcagcgt taatcattgt agctgcgtca cgaatgcttt cttcgctaaa ggcgggtgca
    12181 acggcttttt ctgccaccgc gggctgagct tcaatctcaa aaaccgccgt ttgtacatcc
    12241 ttaggaatgt ctatccacac cgggctaggg cggcctgatt gcgcaatgcg gaaggcatcg
    12301 ctcatgacct gtgggagctc ttcgatatgt ctgaccagat agttgtgttt ggtgatgggg
    12361 atagagatgc cgtaggtgtc cacttcttgg aaggcgtcgg tgccgatcat cgaggcggga
    12421 acctgaccag tgatgcaaat cagcgggatg gagtccagcc gcgcatcagc aatggcggtc
    12481 accaggttag tcgcacccgg cccgctacag gccatacaga ccgccggttt accgtcggtg
    12541 cgcgccattc cctgagcgat aaaccccgcg ccctgttcat ggcgagccag aatatggcgg
    12601 atttgcgtgc tttggcttaa ggcgtcgtaa acaggcagga tagaaccgcc cggaatgccc
    12661 gtcacaatct taatgccctg ctgttccagg aaatgaacga taaattctgc gccggtaaag
    12721 cgcttacgcg tcgatgttgt gcccgaactt gccatgctcc agtccttttc ttctgggccg
    12781 actttccggg aggtgcttaa acgaaaaacc ccgcccggtt tgcgccggcg gggttttgga
    12841 atcgtgtgtt gttccagtcc ctacggcgca ttgccgacga ccaccaccac acgcacgacg
    12901 accactgcgg cggatggcgc agttggtagt agttttgcgt tgaacatgga agtagtcatt
    12961 ggggaccttg ttggttttgt gtttaacaat atttatacaa gcacagcttt acaggggaga
    13021 caatggaaaa tttttcagca agggaaaatt gaggggttga tcacgttttg tactgaattg
    13081 cagataacaa aaaaccccgc cggagcgagg tttcgtcagc cgcctgcggc tggtaaccgc
    13141 aaagcacact gtattatgtc aacactgaaa gtatacgtgt tccgcgcaga acgcgcaatt
    13201 tcggcacgaa ttttgacgta tttagtgcat agttgagtat cgttcacagt ttgcgttttg
    13261 tccaaatatt actgtttatt tatacagtaa gcttctataa tatcactgta cgcaatgtgt
    13321 tatgcggggg ccgcatcgtt acccggcgca ctaagtcctg gctgaaacgg gtggtgccgt
    13381 cagcgcctta accccgcgtg agcacactgt gttatgtcaa caagcacaac gtttctcctt
    13441 gagataccgc gtgcacaaca gctggcaaca ggcagcggaa aggtacgtca gctggcagtg
    13501 ctcctgaacc acaggagacg cgtatgagcc tggtggatat cgccattctt atcctcaaac
    13561 tcattgttgc agcactgcaa ctgcttgatg ctgttctgaa atacctgaag taattcagat
    13621 tcaagtcgca ccaaagggga gcgggtaacc gctccccttt tatatttagc gtgcgggttg
    13681 gagtcggatg cgatgctgac gcatcttatc cgccctacca tccctcccgg caacatttat
    13741 tgccgctttt gtttacatat tctgccgcta aacaattccc cattcctggc gtatatctgg
    13801 ctaacattca tcaatgtgat agattcctct cccgcattta tgggaatgcg tagtgactta
    13861 ttctaattat ttttataaaa gcatccgtga taatgaaaag gcaaagaaac gtcaatttgt
    13921 tattgatgtt ggtattactc gtggccgtcg gtcagatggc gcaaaccatt tatattccag
    13981 ctattgccga tatggcgcgc gatctcaacg tccgtgaagg ggcggtgcag agcgtaatgg
    14041 gcgcttatct gctgacttac ggtgtctcac agctgtttta tggcccgata tccgaccgtg
    14101 tcggtcgccg accagtgatc ctcgtcggaa tgtccatttt tatgctggca acgctggtcg
    14161 cggtcacgac ctccagtttg acggtattga ttgccgccag cgcgatgcag gggatgggca
    14221 ccggcgttgg cggcgtaatg gcgcgtactt tgccgcgtga tttatatgaa cggacacagt
    14281 tgcgccatgc taacagcctg ttaaacatgg ggattctcgt cagtccgttg ctcgcaccgc
    14341 taatcggcgg tctgctggat acgatgtgga actggcgcgc ctgttatctc tttttgttgg
    14401 ttctttgtgc tggtgtgacc ttcagtatgg cccgctggat gccggaaacg cgtccggtcg
    14461 atgcaccgcg cacgcgcctg cttaccagtt ataaaacgct tttcggtaac agcggtttta
    14521 actgttattt gctgatgctg attggcggtc tggccgggat tgctgccttt gaagcctgct
    14581 ccggcgtgct gatgggcgcg gtgttagggc tgagcagtat gacggtcagt attttgttta
    14641 ttctgccgat tccggcggca ttttttgggg catggtttgc cgggcgtcct aataaacgct
    14701 tctcaacgtt gatgtggcag tcggttatct gctgcctgct ggctggcttg ctgatgtgga
    14761 tccccgactg gtttggcgtg atgaatgtct ggacgctgct cgttcccgcc gcgctgttct
    14821 ttttcggtgc cgggatgctg tttccgctgg cgaccagcgg cgcgatggag ccgttcccct
    14881 tcctggcggg cacggctggt gcgctggtcg gcggtctgca aaacattggt tccggcgtgc
    14941 tggcgtcgct ctctgcgatg ttgccgcaaa ccggtcaggg cagcctgggg ttgttgatga
    15001 ccttaatggg attgttgatc gtgctgtgct ggctgccgct ggcgacgcgg atgtcgcatc
    15061 aggggcagcc cgtttaagcg cacgtcaccg cagcatcgtc atcagctcca tgggagaacg
    15121 atgctgcttt atcagatcac gcatcacccg catatgcggt gcggagtaag aataaaacgc
    15181 ctgatagccc gcacaaagca cgctgtctgg cgtctcctgc cgagcgtgcg ggcacaggcg
    15241 atgccagtcg cacgcctggc agtcacggcg cggttgcggg ttttcgctgc cacaccattt
    15301 ttgtaaggtg gcgtcaaaaa gctcaatcga gatacgactg atatctgccc gcacccaaca
    15361 ctcaaacagg gcaattaaaa atttccccca cagctctgct gtcaccgaag cggccgtcag
    15421 atcgccccgg tcatcaaatt caagataggg aatgagctgt acggagcgga tgccccgctg
    15481 tttgaaattg tgataaaaat cggcaggttg cagagcatcc ttgtactcta caaccagctt
    15541 atgcctgtcc tcttcagcat caatgacctg acttcctgtc atgcgactct ctcaataaat
    15601 acgataagtt gatgtatatg cgttacagca aacagtattg cgagagataa caccgcgagg
    15661 gagatcaaaa atttgtcacg gactacgtga aattgggaaa aatcgctatt cgtgacatcc
    15721 attaaattgc gattacgggt gtagtgccag aggatcagcg ccacgatggc gaggatgcca
    15781 atcgaaatcc agaataacat gcccgcctgg tgccagttgt gtttgatagc caacgccatc
    15841 aacgcgccgt agcccagcat ggtacgaaac cacgccagtg atgtgcgctc cggctgcagc
    15901 cctgggtcgg caatgcgtct ggctttacgg ctatccggca tacaacacca gtcccataac
    15961 gatcaccgcc acaaccatca atattaagct gatgattaat aagctgttgg tgtagggcag
    16021 gtcctctttc agacgcatcg ctttttcatt gcgtaaccag cgcaggtagc catacattgc
    16081 taacccgccg gaaaacagac acaacaacaa cgccagcagt tcacgaatga cgggcgtggc
    16141 gaaatctggc gcaagttgat caagcccaac ccccgccgcc agaaatccta gtgcggtacg
    16201 gatccacgcc agaaaagtac gctcattagc cagcgagaag cggtaatcgg gcgcttctcc
    16261 aaggcgggaa attttcattg ttgctccttt gcaatttcac taacgctcta tctttagcgg
    16321 ttttttttaa gaatttcgtc cgcccgtttc ctgatatgct tttgccatcc gctacaagga
    16381 ggtcagatgg gtatcatagc gcaaaataaa atcagttcat tgggcatgct gttcggtgcc
    16441 atcgctttaa tgatggggat tatccacttt tcattcggcc ctttttccgc accaccgcca
    16501 acgcttgaaa gcatcgtggc ggacaaaacg gctgaaatta aaagaggtct tctcgccgga
    16561 ataaaaggcg aaaaaattac cactgtggag aaaaaagagg acatggatat cgataaaatc
    16621 ctcgatcaaa gcggtatcgc actggccatt gctgcgctac tttgcgcatt tattggcggt
    16681 atgcgtaagg aaaatcgctg gggcattcgc ggcgcgctgg tttttggtat cggcatcgtg
    16741 tgcagcattt tgctgatatt cctgattttt tccttcctga ccggcggttc gctggtttaa
    16801 gcagcctgaa taatgcccgc cgggtgatca tcagcgggca ttatcgtcag aatttctgtt
    16861 ttttctcctc caccttcact ccttgcgtcg gtaggccggt gtcatagtcg cgtaccactg
    16921 gcgaatagcc atcttgtggg cgtgggcgaa acgcccccat ccagcgcggt cgtgcatctt
    16981 tacgccacgg acgcagactc cattggtaac tgcggaacgg atcgcgaatt ttgtccatgt
    17041 aatccaataa ggcgtcatgc attttgctgc gaacgtctgc aaaacggata tcatcgatca
    17101 ggttatgcat ttcatttggg tcattacggc gatcgtaaag ttcatcactg gtgaagaggt
    17161 tgagcaccag tttaaagtca tccgtcaccc agcaacgcac cggaataaaa ccgccaaagc
    17221 tgtcatgctc aatctcgtag cggttaaatt ccaccatcac gccgcgtggc tctttcacgg
    17281 caaggatatt ttcccccggc agaatctctg gtttttcaat atctgccagc gccatcattg
    17341 tcggcagtaa atcaatatga ctgactggcg tatcgacctg tcgccgctcc ccttgcggcg
    17401 aacggatgat cagcggaata cgggtgatgt cgtcatacat cgccgctcct ttactgatca
    17461 gcttatgtgc gcccatcatt tcaccgtgat cggaggtata aatgacccac gtattttcac
    17521 gttgctctgg cgttaaggca ttgatgacac gtccgatttg gtcatcaaca aagtcattgc
    17581 aggcaaaata gagcggatgg tgataaagcc cgtcatcacc gactggcgat ggcatcgcct
    17641 gcgcccataa gcggtgatgt tccggtttgt tcgccaggtc atcctgtgat ttctcgccaa
    17701 gttcgtagta aaaatcagcg tatttctcta aatactccac cggacaggtg aacgggtgat
    17761 gcggctcatc atacgaaacc accatcagga agggttcctc ggcgcgcacg ggctattgca
    17821 gaaaatctac cgctcgattg ctgatgcggt gcgcccaggt gaaggtttcg tcaatatggt
    17881 tcgcctgtaa atcttcgacg ctgtttaggc cattgcgcca caggctgatc tctttttccg
    17941 tcagttcgct aagatagttc gccccatcaa accagtaatc agcatcccac tccggcggac
    18001 actcgccagt gccgaaatag tcatgaccgt cgagatgcca tttgccgatg taacaggtgt
    18061 gatagccggc atccttaaag tagcgcccca tagtggagat gtttttgcct ggcgcgacgt
    18121 tgttggtcca cgggccggac tggttagcgt agataccggt gaatagtccg gcccgtgcag
    18181 gcgtacaaac cggtgaacaa gtgtaggcgg aattaaagcg aataccttcc gccgccagac
    18241 tatcaatatt ttgcgtattc agcggtttac cgctatagca accgaccata ttggtggcct
    18301 gggtatcggt caattggggc gtttcatagg ctatccttcg cgtcgggagt aaaggttacg
    18361 gctgggtcgt gacgatgcca ccagctgtca aaaatcaggt agacaatcac tacggcagcg
    18421 atgaaataac tgatcatcgc taaccagcgc gtaccgtagc cgccgaattc agccagcccg
    18481 acatagacgc caatcatcgc gaacaggata ccaattgacg cgatcttgac gtttttccac
    18541 ggtttcatgt ccaccgcaaa cgcatctttg aaggtgaacg gcgtggcgcg cggtttgata
    18601 aaaccgatca ccagcatcac gaccacgttg atgcagaacg tacaggccag cacatagagg
    18661 aaatggaagt cgaacttcac cagatagttg atggtgatgt agctgattat gccaatcccc
    18721 atcgccactt ttgccgccag cgccgggatg cgcggaaaga aaaagcccat aatgatgatg
    18781 gtaaccagcg gcacgttgta aatgccgttg agctgtttca tccagctata cagcccctgc
    18841 ggcgcgttcg cgatccacgg cgctaccaaa accgaaacga tagcgataaa gaaaccaaat
    18901 ttgcgcccga cggtgaccag ctgctgcggc tcggcattct ggttaatgat gcgacggtaa
    18961 atacccatac tgaataatgt actggcgcta ttcagaaagc cgttgaaggt actgatcacc
    19021 gcaccaaata acaccgcgcc gaagaaaccc tccagtggca ctggcagaac gttattgacc
    19081 agcgtcgggt aggccatgtc ggctttcggt agatcctgat acagatgaaa cgcaatcaac
    19141 cctggcagca ccagtaccag cgggtcgagc attttcagca ccgccgttaa cagcgccccc
    19201 ttttgccctt ccgccaggct ttttgacgcc agcgtgcgtt gcacgatgcc ctgatttgta
    19261 caccagtaaa aggtgttcac cagaattaaa ccggtaaatg ccgcgccaat cggcaaggga
    19321 tcggtcgggc caccgactga gtttaatttc tcggcgtgaa cggtggtaat ttgctcaatg
    19381 ccctgcataa agctgccctt gcctatcgcg atcaggccaa ataccggcac catcaacccg
    19441 ccgataacca gcccaatacc gttgatggag tccgccactg ccattgcgcg cagtccgccg
    19501 atcaccgcat acaaaattcc cgccagacca agcaaaatta ccagtagcca gatagccgca
    19561 ccgtgagaaa tctgtagcga ttccccgacg tgaaacaggc tgttgagcgc caacgcgccg
    19621 gagtagagaa caatcggcag aaagcagacg ccggtggcaa tgaggaagca gaagtcgatg
    19681 ataatacgcg tcgttttatc ataacgttcc tccagaaaat cggggatggt ggcaatgccg
    19741 cgcttcagat agcgcggtaa aaagattagc gcgaggaaga tcagcgtcac cgctgaagtc
    19801 acttcccagc ccatcaccga catgccgctt ttgtaggcct gcccggaaag accaaccagt
    19861 tgttccgtgg aaaggttggt taacattaac gaagcggcaa taaccggcgc ttttagtgaa
    19921 cgtccggcaa gaaaatagcc ttgttgcgat ccggtatctg ttttgcgaac cttccaccag
    19981 gtgatcaccg ccaccagcag cgtaaaaccg acaaaactca agatttgtaa cgaattcatc
    20041 tcatagccct tatttatgtt tatgtcccgg acgcatgaat atagggagag gtaaaatgcg
    20101 tcgggtcagg cagtcagatt cttaaaatac aaacgtcgta tccctgaacg gattgttttc
    20161 tgttaagttc aggttgtgag cataattcgc cggagggata ttaaaatgaa tggaaaattg
    20221 caaagttcag atgtaaaaaa cgaaactccg tacaatattc ccttattgat taacgaaaat
    20281 gtgatctcca gcggaatttc tctgatctcg ctctggcata cctacgccga cgagcattac
    20341 cgggtgatct ggccgcggga caagaagaaa ccgcttatcg ccaactcatg ggttgcggtg
    20401 tataccgtac aaggatgtgg gaaaattctt ttaaagaatg gcgaacaaat aactctgcat
    20461 agcaactgta ttatattttt aaagccaatg gatattcact cttatcactg tgaaggttta
    20521 gtctgggaac agtactggat ggaatttacc cccaccagta tgatggatat ttccgttggt
    20581 cagcaaagcg ttatttataa tggcgaaatt tataatcagg aactcaccga agttgctgag
    20641 ttaataactt caccagaagc aataaaaaat aatctggcag tcgcttttct gacgaaaatt
    20701 atttatcagt ggatttgtct tatgtccgca gatggtaaaa aagatccaca acggcggcaa
    20761 attgaaaaat taattgccac tttacatgcc agtctgcaac aacgctggag cgtagctgat
    20821 atggctgcca cgatcccctg tagcgaagcc tggttgcgtc gtctgttttt acgctatacc
    20881 ggcaagacgc cgaaagaata ttacctcgat gcgcgtctgg atctggcgct atcgctatta
    20941 aagcaacaag gaaactcggt tggcgaagtc gctgatacgc tcaacttctt cgactccttt
    21001 catttcagca aagcctttaa acataaattt ggttatgcgc cgtcagccgt gctaaagaat
    21061 acggaccagc acccaacgga tgccagtcca cacaattaac gtaactctgg ccagtacgct
    21121 ttgttggcct ccagcaactc atccagaata gctttggcga ccgaggcgct cggtaccgtt
    21181 ttcgacaacg taatcgcctg ccacaggtgc tgatatgaac gctgttccca ggcgtccacg
    21241 accagttttt ccaccgccac ttgctgactc attaaccctt tctgaaactg cgggatatcg
    21301 ccgaccacca gtggttcagg ccgtagtagc gcacgcgcat ctgtttgcga tcttgcagcc
    21361 caacaatttg cgccatccgg ctttcaatac cgattggcat gtcacagatg ttgaggattt
    21421 tcgcattcgg gcgcagacga cgcgtggctt ctgcgacaat tgctgccggg ttggagtagt
    21481 tgagcatcta ggcgtttggt gaatattttt ccatataatc caccagttcc aggacgccgc
    21541 caatggaacg catgccgtac gctattccgc cgggtccgca agtttcctga ccaacaacac
    21601 cgtggcgcag tgggattttt tcatccagtt cgcgcatcgg gtacttgccg acgcggatgt
    21661 gcgccatgac aaaatcaacg tcgctgaacg ccacttcagg atcggtggtg taactaaacg
    21721 caatgtccgg cgctttttct ttaaggatga ctttacaggc ttcggcaatc acttcctgcc
    21781 gcgcgccatc gttatcataa aatttcagtg cacgaagcgg gaaacggtcc tgattcgcca
    21841 ggagcatcaa cacgatgcct ggcgtaaagg tgcttccacc gcctgcgaca accactgaga
    21901 atttggtcat tatactgcct ccgtaatggc aacattttct gctgattgat gagaattaat
    21961 taagctatcg agctgttcac gcaattggga tacatgcaga ccaatgatca cctgaatggc
    22021 atcgccacta cggaagacgc cgtgcgctcc cagcttttta aagacttcgt catccagcgt
    22081 ttgtgacatg tcatgcagtg caatacgtag acgcgtcgcg caattgttaa tgctggagat
    22141 attgccgacc ccgcccaggg cttgcaggat accggcagcc tgatccagct ctttttttgg
    22201 ctctgcagcg gtggtttggc ctcgcgaggc tttgtattcg gcttttgagt agagtttcac
    22261 ttccgcatct tcacgtcccg gcgtgcacat gttgaactgc agaatcaggg tgcggaaaac
    22321 cacgaagtac agcagggtaa agcacaaccc aatggcgatt tgggtcagca tcatatccgc
    22381 gtggttgctg aacatcggga tccagttttg cggtaaaacc tggtcaatca gacctccgcc
    22441 catgttgccc accacaccaa agagatacat tacggtcgac attgaggccg ccagcacggc
    22501 gtgtaccgca aacagcaacg gtgaaatgaa caggaaggta aattccagcg gttcggtaat
    22561 tcccaccagc atggcggtta aggttgcggg aatcagcaag cccgccactt ttacccgatt
    22621 ttccggtgcg gcagtgaagt acatcgctaa agaaatgccc acggcaccaa agatttttga
    22681 gttaccgtgc agggcaaaac cgccttccgg gaacaacgat ttcagtggct cggcgctcag
    22741 actgaactct tgcagatgct gcgcccagta catctgaatg ccgccttcaa cagctgccgg
    22801 accaaagata aacggtccgt agatgaagtg gtgtaaaccg gttgggatca gaatacgttc
    22861 gaggaaggtg taaacccaca ccccaagcgc acccgccgaa cgcaggaacg cttgcagaga
    22921 ttcaatcccc atttgtactt ttggccagcc gagcaatgtc agccaggcac aggggatcat
    22981 caccaggaag gcgataatca ccacataaga cgtcccctgg aaaatgccga gaaaaaccgg
    23041 cagtttttta tcgaacagac ggttatgcag cgccgtcaca atgccggaaa tgataattgc
    23101 gccgataatg ctggtatcga gggttttaat cccggccatc attgtcagac cgctacctgc
    23161 caccgcgtcc tgagtgaaat cgacgccgaa gtagcttccc caggtcattc ccatcgcatt
    23221 gatgaaatag ttccaggtca ggaaactcac catcaccgcc agacaagcac gtccctgcgc
    23281 ttgcttagca aggccaatgg gtaaaccgac agcaaaaatc agcggcatat tacggaatac
    23341 cgtccaaccg ccctcttcaa taatgtgtac gatttgcgcg aataaactgt tcggatcggt
    23401 cagtgattcc ccgacaaaca tcgggttttg cagcaagatg gcaagaccca ccacaatccc
    23461 ggcgaaggga aacagcagca ctggcgtgaa catcgcgccg ccaaagcgtt gaatttgact
    23521 gagcatttcc acatcctctt ttctcaattc tgtaagtagg aagcctgatt ggttatgcgg
    23581 cagacctgga gcgaggatag gaagatgcga tcggcagaac atgacgaccc aatgggattc
    23641 gtgatcgctt tcatgctttt cgttgggcgg tagagatcta cgttttcgcg aaaaagtaga
    23701 catctgtgag cacaggtaca cagcatcgaa cacatcttta aaaaaaagat ttttttcaat
    23761 cgattaagca gaacttgtgg gcgcattacc cgtgcttgca ggcaaaaaag agagctagag
    23821 atgatctaca aaagcattgc ggagcggtta agaattcgac ttaattccgc ggatttcacg
    23881 ctaaacagcc ttcttcccgg tgaaaaaaag ctggcggaag agtttgcggt atcgcggatg
    23941 actatccgta aagccattga cctgctggta gcgtgggggc tggtggtccg ccgccacggc
    24001 agcggcactt acctggtgcg caaagatgtg ctgcatcaaa ccgccagcct gaccggactg
    24061 gtggaggtgt taaaacggca gggaaaaacg gtcaccagcc aggtgctgat ttttgaaatc
    24121 atgcctgcgc ctccggccat tgccagccag ttacggattc aaatcaacga gcagatctac
    24181 ttctcccgtc gcgttcgttt tgtggaaggg aaaccgctaa tgctggaaga cagctatatg
    24241 ccggtaaaac tgttccgtaa cctttcgctg caacatctgg aagggtcgaa gtttgaatat
    24301 attgaacaag agtgcgggat tttgattggc ggtaattatg aaagcctgat gccggtgctc
    24361 gccgatagac tgctggcgcg gcaaatgaag gtagcggaac acacgccact gctgcggatc
    24421 acctcgttgt catatagcga gagcggggag tttttgaatt attcagtgat gttcagaaat
    24481 gccagcgaat actaggtgga gtaccattta cggcgactcc acccggaaaa aagttaaccg
    24541 atactccaga agagcaccgc cagtaattgg ggggtgataa tgcgcaggaa catcactaac
    24601 ggatagacag tggcgtaaga gagcgccgcc gcaccactgg ttggatgaag attattagca
    24661 aacgccagcg ccggaggatc ggtcatggaa cctgccagca tcccgcacat ggtcaggtaa
    24721 ttcattttgg ctaacatccg cgccagaatg ccaacagtaa tcagcggaac ggcggtgatc
    24781 agggcaccat aaccaatcca gcttagccct tcgccattga ccagggtatt cacaaaatcc
    24841 ccaccagatt tcagaccaac gaccgagagg aacagcacga tccccagctc ccgcagcgcg
    24901 aggttggcac ttggcggcat aaaccagtac agcttgccga tactgccgat acgcccgagg
    24961 atcaacgcca taatcagcgg accgcccgcc agccccagtt tcaacgcggc cgggaatcct
    25021 ggcacaaaga cgggaataga acctaacaat acgccaagcc cgatgccaat aaacactggc
    25081 agcatctgaa cctgttgcag tttttgctgc gcattcccca gcacattggc aacggcatca
    25141 attgcggacg gacgccccac caggttgagg atatcgccga actgcaggct gatatcgcca
    25201 ctggcgacca gttcgacccc ggcacggttc aggcgcgaga taacaacgtc atagcgttct
    25261 ttaaagtgca ggtcgcgaat acgttttccg agcacgtttt cattggtgac caccacacgc
    25321 tcgacgcgca aatcagtgcc tttcgtagac agcgaggtat cgacctcctg accaatcacc
    25381 agttgcgcat tatgtaaatc cgctggctgc cccaccagat gcagcaaatc gcccagttgg
    25441 ataatggtat ctggcgaagg tacttttagg gtttcttctc gtttcagacg cgagcagata
    25501 attttgtcgc cgttgagaat cggtacatct ttaatggcta aatcatgcag gttagggttc
    25561 tcaacgcgaa tattgatagt cttgatcagc gcgccgccat tggtgcgtga agactcgtgc
    25621 tgctgagctt ctgtctcgac attgacgcgg aaaataaccc gcaacatcca catggtgaac
    25681 aaaatcccgc aaatgccgaa tggatacgcc atcgcgtaac tcatccccat ctgatcgacc
    25741 atttccattg gtgtacccag gtcgcgcaag atctgctgcc ctgcccccag cgctggcgta
    25801 ttggttaccg caccggagaa aatccccagc actaccggca gtggaatatc aaacagttta
    25861 tgcaggatgg cggtaaccag accaccgatg atgacgatca gaacagcaaa caggttgagg
    25921 cgtaatccgg agacgcgcaa tgaggcaaag aagcccggcc ctacctgaat cccgatggta
    25981 taaacgaaca ggatcaggcc aaattcctga ataacatgca gcatatcgct acttaatgtc
    26041 atccccgcct gagaaacaaa atggccgacg atgatcccac caaacagcac gccgccaata
    26101 cctaatccta tgccgcgaaa tttgacgttg ccgataaaca aaccgacgac tgccaccaaa
    26161 gccagaatac tgaccgttaa tgctatatca ctcatcgtcc atttctcttg agataacatt
    26221 ttaattgcta cggattatgg ctgaatcggt gaagcagatt tgccttatag cgcacattat
    26281 cacgggaaag aacgtgccga aatatcttaa acagtcgaaa tttaacgcat tgttgaagaa
    26341 gaaagacaaa aaaagcccca ccagaatggc ggggcaaaga gaatagctag ttagctattt
    26401 aacgcgggac gttcgctgat agcgatacgc tgcgctgcga tgggttcagg ctcattacga
    26461 attaaatcaa tatgcagtaa accgtttacg aaggttgcgc cagagacttc catattttca
    26521 gccagcgtaa aactcaggct aaatggctga ttcataagcc cttgatgcag ccattttttc
    26581 tcttcttttg gctgctccgg cgtgcctttt acgctcaggc gcgtgccttc cagttgaatc
    26641 tctaaatctt cctgacggaa acctgccagc gcaagggtaa tgcggtagtg gttatcgtcg
    26701 cttttctcaa tgttgtacgg cgggaagctc tggctttcac cggcgttttg cagtgcgttg
    26761 gccagtttgt caaaaccgat ccattgacgc atcagtgggg ataaatcgaa gttacgcata
    26821 gtcatttctc cttctaagaa gcgagtaagt acctgcaaat ccgaagattc gcatatgctc
    26881 cctgacggcg agcatggaga tgtcaggccg cgccaggcgg ccttagggaa ttagttgatt
    26941 tcgatacggc gcggtttttt cgcttccgga atcacgcgtt cgagatcgat atacagcaaa
    27001 ccatttacca ggttagcccc acgaacatga atgttctcag ctaactggaa tttgcgttca
    27061 aagttgcgtt cagcgatgcc ctggtacaga taggtgcgct ctttttgttc gtcggcgtga
    27121 gcacctttca ccaccagcag attatcctgg gcggtaattt ccagttcgct ctcagcaaaa
    27181 ccagccacag cgatagcaat gcggtaatgg ttttcgtcta ccagttcaac gttatacgga
    27241 gggtagccgc cattactctg gctctggttg ttttctaagt ggttaaacaa acggtcaaat
    27301 ccaatagcag aacggtaaag cggggataaa tcaaagttac gcataatcaa tagctcctga
    27361 aatcagcgag aatgtaagac cttccacaat ggacaggtca ggtagccaga acacccatca
    27421 ggcgcgtcct catcgactac gatgtaaaat gggtctggaa atgaactttt caagccttat
    27481 cagcggactt ttttttgcag tttatggtct attgcataga ctgagggggc agcacgaaac
    27541 gttaaagtgc gatagccgcc acagtgcgac gtaatgatga tgttattttc agcaagggat
    27601 cgctataact catcaggcca acacccacga tcaacagatg aatttattat gataagaaat
    27661 gtgttgttag cgttcatgat atgcagcgga atgacattac tcggaggatg ctccagcgtg
    27721 atgtcccata ccggggggaa agaaggaacg tatcccggca cccgcgccag cgccaccatg
    27781 attggcgacg atgaaaccaa ctggggcact aagtcgctgg cgattctcga tatgccgttt
    27841 accgctgtaa tggacacgct tttactgccg tgggatgtgt tccgcaaaga cagctcggtg
    27901 cgctcgcgcg ttgaaaaaag cgaagccaac gcgcaagcca ccaatgcggt tattccaccg
    27961 gccagaatgc ctgataactg atttagcgat cagtttcggt gatccacagc tggccacctt
    28021 ccatccacgc cagccattga ccatccggcg agaagaccac ggcgtccgca gaaggtggat
    28081 tggcgtggtt ttcggttaaa tactcaacct cgccgctttg cgcatgggca caaacaattc
    28141 gattatccag cacaaagccc aaccattctc ctgacggatg ccagttaaat gcagactgaa
    28201 tatccgtttt gttatgggtt aacttgcgcg gttcgccgcc ctgtggcgag ataagccaca
    28261 gttgcacaat gccgttatca tcacgcatta aaaacgcgat ttgcgtaccc tgcggattac
    28321 agcgcaccca gtggcgcggg acgttgacta accccggata agcccgatga tgggtaaagg
    28381 ttaaacgtcg ctgcacgacg ccacgcggtg gcgcgggtag ggttgtttcc gttccactta
    28441 acggcgcatc acctgccgat ttccagctag cttcatcttg cggtaactca acgataaata
    28501 gctccggcac tttctcgccc tttggcgaaa gtgtgtcgcc aataaacgcc agcgcgtgat
    28561 ttcctaccca tccttcttca taagcacgat tgatttcatc actgccaggc tgcggcgtgg
    28621 gcgtggtttt actcaccagc acacaccagt ggctaccgct gtattcacgc ggatgttgct
    28681 tttgtacgtt gaccgggcca aacggtgcag caaccccgac gtttcgcaaa tccagcgccg
    28741 gatcgagttg atgcattaca tggtcgttat aggtaaagct ccccctttca ccgttcgggc
    28801 taaagacatg cacatggctg ccaccgcgca gcgctcctgg cgtatacggc gcggtaatat
    28861 ccattgcatc gagattgctc accttgccgc cttcagcaat gactccgcgt cgatgatgga
    28921 agtcgtaatg ccatgtttca tcaggatttt cagggccgtg aataaagaca tatttctctg
    28981 acttcggatg aacggtcacc acgccgacgt gtgcgccctg tgacgcgcga tagataacct
    29041 cgacctcgcc tgtatggata ttcacacgct caatggtctc gccggtaaac gacgccccag
    29101 aaggacgcac gtcaaatacc agccactggc tgtcgggcgt ccaggtattg gtatttgtat
    29161 tggtattggt attggtgagc aggtgattac ggggagcaaa ggttatctgt ttcatggcgc
    29221 ggtcctgata caaaacggtt cccgccatca tacttcacaa ggacttcact ttcagacaat
    29281 agtcttttgc catcgtgatg aatgtgagtg aaaagatgga acatttcgac gtggcgatta
    29341 ttggcctcgg cccggcaggg tcggcgttgg cacgaaagtt agccggcaaa atgcaggtga
    29401 tcgcgctgga taaaaagcac cagtgcggta ctgaaggttt cagcaaacct tgcggcggtc
    29461 tgctggcacc ggacgcgcag cgatctttta ttcgcgatgg actgacgctt cctgtcgatg
    29521 tgatcgccaa tccacagatt ttcagcgtca aaactgtcga cgtcgccgca tcgctcacgc
    29581 gtaactacca gcgaagctat atcaatatta atcgccatgc tttcgacttg tggatgaaat
    29641 cgctgatccc cgccagcgtt gaggtttatc acgatagcct gtgccgaaaa atctggcgtg
    29701 aggatgataa atggcatgtc atttttcgtg cagacggctg ggagcaacat attaccgccc
    29761 gctatctggt cggtgccgat ggcgcaaact cgatggtgcg gcgacatctc tacccggatc
    29821 atcaaatccg taaatatgtc gctatccagc agtggttcgc agagaaacat ccggtgccgt
    29881 tctactcctg catctttgat aatgcgataa ctgactgtta ctcatggagt atcagcaaag
    29941 atggttattt tatctttggc ggtgcctatc caatgaaaga cggtcagacg cgtttcacga
    30001 cgctgaaaga gaaaatgagc gcctttcagt tccagtttgg caaagcggtg aaaagtgaga
    30061 aatgcacggt gttgtttccc tcacgctggc aggattttgt ctgtggtaag gacaacgcct
    30121 ttctgattgg tgaagcggcg ggatttatca gcgccagctc gctggagggg attagctatg
    30181 cgctggatag cgcagagatt ctgcgtgcgg tgttcctgaa acagccggag aagagcaacg
    30241 ccgcttactg gcgcgccaca cacaaactgc gtttaaaact cttcggcaag atagtaaaaa
    30301 gccgatgcct gaccgcaccg gctttaagaa agtggattat gcgcagtggt gtggcgcata
    30361 ttccacagtt gaaagattat ccaacgcgct tcacatcacc caccagcagg atgtaagaga
    30421 gcgcgccaat caacgcgacg gcggagatat aaaccagtgc aggcgcgaaa ccgtaaccct
    30481 gcgccaggta ccccaccacc agcggaacgg tgatgccgcc cagaccaccg gcgaagttaa
    30541 acacgccgcc ggttaaacca atcaggcgca tcggtgccag agaagagacc agcgaccagg
    30601 taatcgaagc aaaaccgttg ccgaagaatg ccagcgccat caggcacata atcatcatcg
    30661 gatcgttggt gtagttagcg cccataatgc aggtggagat cagcaagccg cagataatcg
    30721 gcgttttacg cgcaaagccc agtgaaaagc ccttacgtac cagcagatcc gcgacccagc
    30781 cagagagcag gacgccgaca aacgccgcga ggaatggcac cgtggtcata aagcctgctt
    30841 tcagcgccgt gattcctttt tcctgggtta aatagttcgg gaaccaggtt aagaaaaacc
    30901 acagtgcaga agccaccgca aattgcccaa gataaacgcc gatcagttta cgatggaaca
    30961 ccagtttcca gtctttggct gttaacggct gacgcgcctc tttcttcacc ggcgcatcac
    31021 catccaccag accgccgcca tcacgaatgt aatccagttc agctttgctg atacctttgg
    31081 tcaggcgcgg cggctgataa accttaaacc aaatcagcga ccagataatg ccgataccac
    31141 cagtgacaat gaacacccag tgccagctca acatctcctg aatccagatc agcagcggag
    31201 tcaaaaacgc cagaccgaca aactgaccag acgtataaaa accaacggcg gaagcgcgtt
    31261 catgttccgg gaaccagctg gtcaccatcc ggttattggt cgggaaagcg ggcgcttcga
    31321 aaataccggt tatcgcacgc agaccaatta atgacattaa tcccgtggca aagccttgga
    31381 ataaagtcgc cactgaccaa ccaaatatcg caataaaata agtcacgcga gaacctacgc
    31441 gatctaaaaa ccaaccgccg gggatctggc atagcgtata aagccaggcg aaggccgaaa
    31501 atacatagcc catttccgct ttcgtaatgc cgaactcttc ctgaatatgg gcggaagcca
    31561 cggccaggtt agcgcggtca acataacaaa tgaccaccgt aataaagatc atcaccagcg
    31621 tcagataacg ccgacgcccc ggctttgctg cattaacggg aatatccata gcgagctttc
    31681 tccagatttt gggcatagcg aagccgctca ccatgccctg taatttacag agggttatat
    31741 ttttgtattg ctgttttagt gcccgatgag gggcttacgt ggcaggaatt accactctgc
    31801 tacgctgtta tcttcatgac gccagagcgg attacgccag tccggggcat ttttactgaa
    31861 ctcaataact ttagcttcgt cgatttccac acctaagccc ggtttcgtta acggtttaaa
    31921 gaagccgcca accatgctga agtcttcttt gtttttcaca aagtcgagta actccgcgcc
    31981 tttgttgtaa tgaatgccca tactttgtta ctgaagtacc gcgttatagg aaacgaagtc
    32041 gatatgcagg caagccgcca gtgcaatcgg tccgaacgga cagtgcggcg caagggtcac
    32101 atcataggct tctgccattc cagcaatttt gtagcattcg gtaataccgc ctgcatggga
    32161 gagatccggt tgcagaatcg aaataccgcc tgcctccagc acgcgtttaa aatcgaaacg
    32221 tgagaacatg cgctcacccg ccgccagtgg aatatgcgtt tgtgccgcca atttcgggta
    32281 gtattcggct tgttccgcca gcaccggctc ttcaataaac agcgggcgat acggctccag
    32341 ttctttaatc agcactttcg ccattggcgc gctgacgcga ccgtggaaat caagaccaaa
    32401 ctcaatctga ttgccaaaag cttcacgaat ttgtgccacg gtgttgactg ccgcatctac
    32461 cgcgcgggag ttatcaatta gccccagttc ttcacaaccg ttcagtttga aggtatcgaa
    32521 gccgatttcg cgcagggttt taatgccgtc gataacatcc gccggacgat cgccgccgac
    32581 ccaactgtag gctttaattt tgtcgcgaac caggccgccc atcagttgcc agactggcgc
    32641 attcagcact ttgcctttga tatcccataa cgcctggtcg atcccggcga tggcgctcat
    32701 caggattgga ccgccacgat aaaatccggc gcgatacatc acttgccata agtcgttgat
    32761 gcgcgaagga tcctgaccaa tcaaatagtc acccagctcg tgaactgccg cttccaccgt
    32821 acgggcgcgg ccttcaatca cgggctcgcc ccaaccgacc acgccttcat cggtttcaat
    32881 tttcaggaac atccagcggg gaggtaaacg atacgtggta attttggtga ttttcattgc
    32941 actgcctctc gatacgcctt aacaaatgct gctgcctgct gcgcggtgcg ttctacggac
    33001 tgcccggcgc gataaagatc gctgcctaag cccgccccaa cacaacctgc gtctatccac
    33061 tgcgccaggt tttctggcgt cacgccgcca acggcaaaga ctgcgatgtc cggtggtaat
    33121 accgctttta acgctttgat gtattgcgga ccaaaagccg atgacggaaa tattttcagc
    33181 gcctgcgcgc ccgcttcgag cgcggtaaag gcttcggtcg ccgtcgcgca gccggggcaa
    33241 acggtcatgc cgtagcccac cgcgcggcgg atcacttcac tatggatatt gggcgtaacg
    33301 atgagctgac agcccatcct ggcgagcgca tcgacctgtt caggtttcag taccgtacct
    33361 gcgccaatca acgccttgtc gccatacgca tcaacgatgg cgggaatgct ctgctcccat
    33421 tgtggggaat tcagcgggat ttcaaccgcg tcgaacccgg cgtcaatcac cgcgccaaca
    33481 tgcgccagcg cctcgtcggg cgtaatgccg cgcaaaatgg cgatcagcgg gagtttagtt
    33541 tgccactgca tgagcgatgc tccttatacc agcctgaaat gccgtgtcgc ccgccaccgc
    33601 cgccacgtcg caacccatcg cctgaaaggc ttgctggtag cgcgcggtca gcgatgttcc
    33661 ggcgacaagg gtgatggcgt gttgatgggt cacatagtcg cgcatactgg caacctctgc
    33721 gccaatcaac aaaccagaga gaaattcgct gacctgttcg cggggaagtg ttcccagcac
    33781 atgcgaggcg cgaacttcaa aaagctgcgg caatatggcg ggcgcattaa ggccgcgctc
    33841 aaggccagcc gcgaaggcat cggcagagtt ttcctgcggc ggcaaacctg cgccaatcaa
    33901 tgagtgattt aacagtaaat gatgtaattc accggtcatc acggtgcgaa aatcgttgat
    33961 ttgctggcta tcggcctgca cccatttgca atgcgtaccg ggcataacat aaagagagga
    34021 aggagccaga gctcgcgcgc cgatcaattg tgtttcttcg ccgcgcatca cattgtggtt
    34081 atcgtcatga gagacgcata atccgggaat aatccagata ttgtcgccaa cagacgttaa
    34141 ttgttcgcca atagacgaaa aacgggcagg aacagataaa tacggagcaa ctttccagcc
    34201 gacattgctg ccgaccattc ctgccatcac cactggcgta ttttcttcac gccagtcggt
    34261 cgtgacttct gctaacactg cagccggaga ttttccgttc aggcgcgtga cgcctgcttc
    34321 tgattgcctg ctctccaggc agtggtcgcc ctgataaagc caggcgcgca gattggtcga
    34381 tccccagtca attgcgatgt agcgagctgt catgtgattt cctttaacct tcgtgtcgag
    34441 ctggcaatca tggtaagcgc cgcctgctct gccgcatcgc cgtcctgatg ccgtatcgca
    34501 tcgaacagcg ccttatgttc ctggagcgtt tgcggcatgt tggcctcatc gcccatccag
    34561 gttcgttcaa aaaccgcccg ctgcagcgaa ctgatcgcaa tgctaagttg ctgtaacacc
    34621 gggttatgca ccgactgcag caccgcctcg tggtagcgaa tatccgcttc gttaaacgct
    34681 tcgcggtcct gattgttggc aatcatctcg ttcagcgccg attcaatctg cgccagatcg
    34741 ctggaggtcg cgcgctctgc tgcccaacgg gcaatcgccg gttccaccag atttcgcact
    34801 tcgctcatgg cactgataag ccgtgggtcg tagtcgtttt ccagcaccca ttgcagtacg
    34861 tcagtgtcga ggtaattcca ctggttacgc ggtgccacaa acgccccgcg ataacgtttc
    34921 atttcaatca gccgcttcgc catcagcgaa cggaacacct cacggatgat gttgcgcgag
    34981 gttgcaaact cttcacagag ctccgcctca gccgcaagtg gcgagccagg tacgtatttg
    35041 ccgtgaacga tctgtttacc cagcgtaatg acaatgcgat cggttttatt gagagtcatg
    35101 gagagtcctt gtgctctgta tgttcttctc tactttaccc cgatcgatgc ataacgcggc
    35161 aactttgtag taccagcgtg atgacgttcg cgtttgccgc gcgcgtaatg tagtacaaac
    35221 ttatattgtt gcgctacaat ttagatcaaa aaaagaacaa cacataaaaa aatgacatgc
    35281 gtcgggcaga aatctgaaaa gggatatcag gcgctaaaca ggagggaaag aagagtatgc
    35341 tttcaacggc ttagctactc gtttaaagga ttaatcatga agttgaattt taagggattt
    35401 tttaaggctg ccggtttatt cccactggcg ctgatgcttt caggctgtat ctcgtatgct
    35461 ctggtttccc ataccgcaaa gggtagttca ggaaagtatc aatcgcagtc agacaccatc
    35521 actgggctat cgcaggcaaa agatagtaat ggaacaaaag gctatgtttt tgtaggggaa
    35581 tcgctggatt accttatcac tgatggtgcc gatgacatcg ttaagatgct caatgatcca
    35641 gcacttaacc ggcacaatat tcaggttgcc gatgacgcaa gatttgtttt aaatgcgggg
    35701 aaaaagaaat ttaccggcac aatatcgctt tactactact ggaataacga agaagaaaag
    35761 gcactggcaa cgcattatgg ttttgcctgt ggtgttcaac actgtaccag gtcactggaa
    35821 aacctaaaag gcacaatcca tgagaaaaat aaaaacatgg attactcaaa ggtgatggcg
    35881 ttctaccatc catttaaagt gcgattttat gaatactatt cacccagagg cattccggat
    35941 ggtgtttccg cagcattact gccagtgact gttacactgg acatcattac tgcaccgctg
    36001 caatttctgg ttgtatatgc agtaaaccaa taatcagtaa gcgggcaaac gcgtttatgc
    36061 tgtttgcccg cccacagatt aattcagcac atacttctca atagcaaacg ccacgccatc
    36121 ttcaaggttg gattttgtga caaagttcgc cacttctttc accgaaggaa tagcgttatc
    36181 catcgccaca ccgacgcctg catattcaat cattgcgatg tcgttttcct gatcgccaat
    36241 cgccatgatt tcttccggtt taatacctaa cacgtcggcc agtgatttca cacccgtacc
    36301 tttgttaacg cgtttatcga ggatttcgag gaagtacggc gcacttttca gcacggtata
    36361 tttctctttc acttcctgcg gaatacgcgc gatagcctgg tcgaggatgg cgggttcatc
    36421 aatcatcatc actttcagga actgggtatt ggggtccatt ttctccgctt cgcagaacac
    36481 cagcggaatt gtggcaacga aggattcatg caccgtgtag tagctgatat cgcggttagc
    36541 ggtgtacagc gtggtgcggt ccagggcgtg gaaatgagaa ccgacttcgc gagagagttt
    36601 ttccaggaaa cgatagtcgt catagctgag agcggtttgc gccacggtgc taccatcagc
    36661 ggccttctgt accagcgcgc cgttataagt aatgcagtag tcgcccggct gttccatatg
    36721 cagctctttc aggtagttgt gcacacctgc atacggacga cccgtcgtta gcacgacatt
    36781 cacgccacgg gcgcgagctg cggcaatcgc atttttaacg gcgggtgaaa tggtgtgatc
    36841 gggcagcaga agggtgccat ccatatcgat agcaatgagt ttgatagcca tgagttccca
    36901 aggtagatta gttcctgacc catgctaacg cgattccgct caaaaatcag tacaacaccc
    36961 gagggaaaag gggatgcaac gcgggttgca tgctccccat ttgcttagcg gaagagtttc
    37021 cctttcagca gttccatgcc tgcggaaagc agatcgttat tggcttgtgc tgacacttca
    37081 ccttgcggtg agagcgcatc aataatcttc ggcaattgct ctgccagtaa actggaagcc
    37141 gtactggtat ccacgccaag tttttgcccg agatcggaca ccgcatttgt gccgagcgcc
    37201 gactccagtt gctcgccgct aactgattga tttctctgtt gattactcaa ccaggttgag
    37261 agaatggccc ctaagccgcc actttgcagt ttttccagca gcacctgaat gccaccctgc
    37321 tcctcaaccc aacttaaaat cgcctgatat ttccccgcat cgcctttcag aaaggcaccg
    37381 acaacttcat caaaaagccc catgataatc acctgtaaag cgttacgtgt tgacccaaaa
    37441 agtatagatc tgcgggatga taattgcgga ttgcagaaat aaaaagggcg gagatgatct
    37501 ccgccctttt cttatagctt cttgccggat gcggcgtgaa cgccttaccc ggcctacaaa
    37561 atcatgcaaa atcaatatat tgcaggagtc tcgtaggact aataagcgta gcgcatcagg
    37621 cacgctcgca tggttaacgc cattaaatat caatattcgc cgctttcagg gcgttctctt
    37681 caataaacgc acgacgcggt tctacggcgt cgcccatcag cgtggtgaac aactggtcag
    37741 cggcaatcgc atccttaacg gtaacgcgca gcatacgacg gctttccggg tccatggtgg
    37801 tttcccacag ctgttccggg ttcatctcgc ccagaccttt ataacgctgg atggagaggc
    37861 cacgacggga ctctttcacc agccagtcca gcgcctgctc gaagctggct accggctgac
    37921 gacgctcgcc acgttcgata aacgcatctt cttccagcaa gccacgcagt ttctcaccca
    37981 gcgtgcagat acgacgatat tcgccaccgg tgataaactc gtgatccagc ggatagtcag
    38041 tatccacacc gtgggtacgc acgcgaacaa tcggctcgaa caggttttgt tcggcattgg
    38101 tgtggacatc aaacttccac tggctgccgt gctgttcttt gtcgttcagt tcgctgacca
    38161 gcgcgttcac ccagcgggta acggtctgct catcagaaag gtcagcttcc gtcaacgtcg
    38221 gctgatagat aagctctttc agcattgctt tcggataacg acgctccatg cggttgatca
    38281 ttttctgcgt cgcgttgtac tcagatacca gtttctctaa cgcttcgcca gccaatgccg
    38341 gtgcactggc gttggtgtgc agcgttgcgc cgtccagcgc gatagagatc tggtactgat
    38401 ccatcgcttc gtcgtcttta atgtactgtt cctgcttgcc tttcttcact ttgtacagcg
    38461 gcggctgagc gatgtagacg tgaccgcgtt caacgatttc cggcatctga cgatagaaga
    38521 aggtcaacag cagcgtacga atgtgcgagc cgtcgacgtc cgcatcggtc atgatgatga
    38581 tgctgtgata acgcagtttg tccgggttgt actcgtcacg accgatacca cagccaagcg
    38641 cggtgataag cgtcgccact tcctgagaag agagcatctt atcgaagcgc gctttctcga
    38701 cgttaaggat tttacccttc agcggcagaa tcgcctggtt cttgcggtta cgcccctgct
    38761 tcgcagagcc gcccgcggag tccccttcca ccaggtacag ttcggaaagc gccggatcgc
    38821 gttcctggca gtctgccagt ttgcccggca ggccagctaa gtcgagcgca cctttacggc
    38881 gggtcatttc acgcgcgcga cgcgccgctt cacgggcacg ggcagcatcg ataattttgc
    38941 caaccacgat tttcgcatca gttgggtttt ccagcaggta ttccgccagc agttcgttca
    39001 tctgctgttc aaccgccgat ttcacctcag aagaaaccag tttgtctttg gtctgggagg
    39061 agaatttcgg gtccggcact tttacggaaa cgaccgcaat caggccttca cgcgcatcgt
    39121 caccggtggc gctgactttg gcttttttgc tgtagccttc tttgtccatg taggcattca
    39181 gggtacgggt catcgccgca cggaagcctg ccaggtgagt accgccgtca cgctgcggaa
    39241 tgttgttggt aaagcagtag atgttttcct ggaagccatc gttccactgc agcgccactt
    39301 caacgccaat accgtctttt tcggtggaga agtagaagat atttgggtgg atcggcgttt
    39361 tgttcttgtt cagatattca acgaacgctt tgatgccgcc ttcatagtgg aagtggtctt
    39421 ctttgccgtc acgcttgtcg cgcagacgaa tggaaacgcc ggagttgagg aacgacaact
    39481 cacgcagacg tttcgccaga atttcatatt cgaactcggt cacattggtg aaggtttcga
    39541 ggctgggcca gaaacgcacc atggtgccgg ttttttcagt ctcgccggta accgccagcg
    39601 gagcctgcgg tacaccgtgt tcgtagatct gacggtgaat tttaccctcg cgctgaataa
    39661 ccagctccag tttttgcgac agggcgttta ctaccgaaac accaacgccg tgcagaccgc
    39721 cggacacttt ataggagtta tcgtcaaatt taccgcctgc gtgcagaacg gtcatgatca
    39781 cttccgccgc cgatacgccc tcttccgggt gaataccggt cggaatgccg cgcccgtcat
    39841 cctgtacaga gacagagtta tcggcgtgaa tggtgacgat aatttcttta cagtgacccg
    39901 cgagcgcttc gtcgatagcg ttatctacca cctcgaatac catgtggtgc agaccggtgc
    39961 cgtcatccgt gtcgccgata tacatacccg ggcgcttacg caccgcatcc agccctttca
    40021 ggactttgat actggaggag tcataagaat tcgacatcaa cgtttctcgc tcatttatac
    40081 ttgggttaat ccgttatttt acccttttcc acggtaaaca tcttcgaatt ttcgtccgac
    40141 atgtctataa cgtgttcagc actgatcgcg ctgacaaaga cctgtgattg cgtcgctttt
    40201 aagcggctgg caagcaaccc gcgacgctca tcatcaagct cagaggcaaa atcatctatc
    40261 aggtagagac accgccgccc gctttcacga gtgaggaact ctccttgcgc cagacgtaag
    40321 gcgcacatca acagcttaag ctgcccacgc gataaggtat cttccaccgg cgcaccgtcg
    40381 gcgcgaatgc gtaagtccgc tttatgcggg ccatgcgcgg tgtaggttag ctggcgatcg
    40441 cgttcaaaat tacgttccag cacctcagcg tactctgtct ctttctccca gccgcgctgg
    40501 aaagagaaag tcagagaaaa ctcagggaga aattgcttac aggtatcatc catatcggcc
    40561 gcgataccgg cgctatactc cgcgcgccag gtgctgattt gctccgccag cgggatcagt
    40621 tctttatccc acgggcgtag ctgttcgtaa cgagtcacct ggcgcagcgc cgcattgcgc
    40681 tgcttgagca atcgcttgag attgctccag gcggtgaaaa atccaggttc gttgtgaaag
    40741 catccccagt cgaggaatgc tcttctgtat ttggggccgc cgttgagtaa agtaaaccct
    40801 tctggcgtta tcaactgcat cggcatcagg tgcgccagtt ccgcgacttt atgcccgtcc
    40861 gtaccgtcaa tacggacttt gctgtcgccc tgtttgtctt tggttaagcc aatcgccgtc
    40921 tcgcgctctt cgccctgtaa tcgcccgtgg agaacaaatg cctcctgctc atggcgaatg
    40981 acgcgaccaa tctgcaaact gcgaaacgcc cgaccatggc cgagcgtata gatggcttcc
    41041 agcacgctgg ttttgccact gccgttggca cctaccagaa agttaaagcc gggagataaa
    41101 gcgagatccg cggtttcaat attgcggaaa tcgcggatca acaagcgggt gagggacatt
    41161 acagtctcat tggcatgaca acataggcag ccgactgtga tgcggcatct tcaatctgca
    41221 cgctggaaac cgaatcggtc agcatcatgc ggacgttttc gcatttcagc gcgttcagaa
    41281 catccagcac atagctgacg ttgaagccga tttccatctc cgcaccgcta taggtaacgt
    41341 cgaggatctc ttccgcttct tcctgttccg ggttgttggc agtaattttc agctggtttt
    41401 cgctgacata caggcgcaca ccgcggaatt tctcgttaga gagaatcgcc gcgcgagcaa
    41461 acgcctgctt gagcagatcg cagcctgctt ccagatgttt gtccgggttc ttcggcagaa
    41521 cgcgacggta atccgggaag cgaccatcca ccagtttcga ggtgaaaata aagtcgccaa
    41581 cgtgggcacg aatgttattg ctgccaatct gcacacgcag cagattgtcg ccgccgtcga
    41641 gcatacgcat cagttcaatc acgcctttac gcggtacgat caccgaatgg cttggcaaag
    41701 attgaccaat tggcattgaa cagaccgcca gacggtggcc gtcggttgcc acggtgcgca
    41761 gttcttcacc ttcggtttca aacagcatac cgtttaagta atagcgaaca tcctgatgcg
    41821 ccatcgaaaa ctgggttgct tcaatcagac gcttcatcgt cgcctgcggc agggtaaatt
    41881 cgacttcgct ctgccagtca tcgaggttcg ggaaatccgc cgctggcagg gtagacagcg
    41941 aaaaacggct acgcccggag cgcaccagca tccgttcacc ttccagctgc acggcaattt
    42001 ccgcgccttc aggcagacca cggcagatat caaagaattt gcgcgccgga acggtcgtcg
    42061 ctcctggctc gtgtggctga accagcgcaa cgcgtgccac catttccatc tcgagatcag
    42121 taccggtcag cgacaacgta ccgtcagcaa cctgtaacag cagattaccg agaatcggta
    42181 gcgtaggacg accacctaac ggaccgctca cctgttgtag cggttttaat aaatgctcac
    42241 gttctacggt aaatttcata ggtttacgat gacaatgttc tgattaaatt tgaaaaatct
    42301 tctttgatat cgtggctctc ttcacgcaac tgctcgatct tacggcaggc atgaagcacc
    42361 gtcgtgtggt cacgcccacc aaacgcatcg ccaatctctg gcagactgtg gttagtcagc
    42421 tctttcgcta gcgccatcgc catctggcgc ggacgcgcca ccgagcggga tcgacgcttg
    42481 gaaaggagat ccgcgacttt gatcttgtag tactccgcca ccgtcttctg aatattgtcg
    42541 atggtgacca gtttttcctg caatgccagc aagtcgcgca gcgcctcacg cacgaagtcg
    42601 atggtgatcg cccgtccggt aaagttggcg ttagcaataa cgcggttcag cgccccttcc
    42661 agctcacgta cgttagatcg tagacgcttg gcgataaaga acgccacttc acccggcaaa
    42721 cgaatgtcat tttcgtcggc ctttttcatc aggatcgcca cacgggtttc cagctctggc
    42781 ggttcgatcg ccacagtcag tccccaaccg aagcgggatt tcaaacgatc ctcaacgccg
    42841 ttgatctctt tcggatagcg atccgaggtg agaatgatct gttgattacc ttccagcagg
    42901 gcgttgaagg tgtggaaaaa ctcttcctga gatcgttctt tattagcaaa aaactgaata
    42961 tcgtcgatca gcagtgcatc tacggaacgg tagtagcgtt taaactcttc gatcgcgttg
    43021 ttttgcaggg ctttaaccat gtcctgaaca aagcgctcgg agtgcatata aaccactttg
    43081 gcattcggct tgcgcgccat aatgccgtta cccaccgcat gcagcaggtg agttttaccc
    43141 agacccgtgc cgccataaag gaacaacggg ttataggcac cgccagggtt atccgccacc
    43201 tggcaagccg ccgcgcgcgc cagttggtta gatttacctt caacgaagtt atcaaacgtg
    43261 tgtttgacgt ttacgttaga acgataggtc ggttctgccg gggccgggac gttatcccaa
    43321 cctgagcgcg tagaaggcgc agcacgttgc ggctgcgttt gcgccacctg tgcaggggcc
    43381 gcgacgttgc tcgtcactgc cgcttgtggc gtttgcgtca ccggtttggt gccgacttca
    43441 aaacgcagct gtggggcatc cgctccgcag aaactggtta gcagtccatt gatattatta
    43501 aggtacttgt cccgtaccca atcgaggaca aaacggtttg gcgcgtacag ggccagcgtg
    43561 ttatcgctca gttccgcctg caatgggcgt atccacatac tgaattctgt ggctggtaac
    43621 tcatcctgca atcgggcaag acactgctgc caaagcgaaa gtgacacggc ggactccact
    43681 cgaacaaaag tcgataatga ctaaggctga aacattcatg attgttgacg tacgtcgaaa
    43741 agaccctgct tgagggtgac gcacgaaccg ctgtctgcgg ttatatgccc gatcaagatc
    43801 ctgcaaaacg atcgggaccg cggatcatag cctaaactgc gcaagagatc ttctgtttct
    43861 cacagatttt ccctatttat ccacaggact ttccagaact cgctaagtgt aaacgatcct
    43921 gccgcgaggc gggcacgatt tacgccgcat attggaaaat ttaatgacca tagacaaaaa
    43981 ttggcttaat cgatctaata aagatccagg acgatccttg cgctttaccc atcagcccgt
    44041 ataatcctcc acccggcgcg ccatgctggt ttccactggt gtgaggtcgt acattttccc
    44101 tgcgaaaagg tgcggaaaag cgcggtaaat aaggaaagag aattgactcc ggtgtgtaca
    44161 attattacaa tccggcctct ttaatcaccc atggcttcgg tgtccatcgt ttcatttttc
    44221 ggcggatatc caataaagcc attgaattta ttcaagttta ggtagaaatc gccatgaaac
    44281 gcacttttca accgtctgta ctgaagcgca accgttctca cggcttccgt gctcgtatgg
    44341 ctactaaaaa tggtcgtcag gttctggcac gtcgtcgtgc taaaggccgc gctcgtctga
    44401 ccgtttctaa gtaataaagc taacccctga gtggttaagc tcgcatttcc cagggagtta
    44461 cgcttgttaa ctcccagtca attcacattc gtcttccagc agccacaacg ggctggcacg
    44521 ccgcaaatta ccattctcgg ccgcctgaat tcgctggggc atccccgtat cggtcttaca
    44581 gtcgccaaga aaaacgttcg acgcgcccat gaacgcaatc ggattaaacg cctgacgcgt
    44641 gaaagcttcc gtctgcgcca acatgaactc ccggctatgg atttcgtggt ggtggcgaaa
    44701 aaaggggttg ccgacctcga taaccgtgct ctctcggaag cgttggaaaa attatggcgc
    44761 cgccactgtc gcctggctcg cgggtcctga tagccctcat tcgggtctat caacgcctga
    44821 ttagtccgct actcgggccg cattgtcgtt tcactccaac ctgttcaagc tacggaattg
    44881 aggcattgcg caggtttgga gtgataaaag gcagttggtt gacggtgaaa cgcgtattaa
    44941 aatgccaccc tttacaccct ggtggtgacg atcccgtccc gcccggacca tttgatacca
    45001 gagaacacta acgatggatt cgcaacgcaa tcttttagtc atcgctttgc tgttcgtgtc
    45061 tttcatgatc tggcaagcct gggagcagga taaaaacccg caacctcagg cccaacagac
    45121 cacgcagaca acgaccaccg cagcgggtag cgccgccgac cagggcgtac cggccagtgg
    45181 ccaggggaaa ctgatctcgg ttaagaccga cgtgcttgat ctgaccatca acacccgtgg
    45241 tggtgatgtt gagcaagctc tgctgcctgc ttacccgaaa gagctgaact ctacccagcc
    45301 gttccagctg ttggaaactt caccgcagtt tatttatcag gcacagagcg gtctgaccgg
    45361 tcgtgatggc ccggataacc cggctaacgg cccgcgtccg ctgtataacg ttgaaaaaga
    45421 cgcttatgtg ctggctgaag gtcaaaacga actgcaggtg ccgatgacgt ataccgacgc
    45481 ggcaggcaac acgtttacca aaacgtttgt cctgaaacgt ggtgattacg ctgtcaacgt
    45541 caactacaac gtgcagaacg ctggcgagaa accgctggaa atctcctcgt ttggtcagtt
    45601 gaagcaatcc atcactctgc caccgcatct cgataccgga agcagcaact tcgcactgca
    45661 caccttccgt ggcgcggcgt actccacgcc tgacgagaag tatgagaaat acaagttcga
    45721 taccattgcc gataacgaaa acctgaacat ctcttcgaaa ggtggttggg tggcgatgct
    45781 gcaacagtat ttcgcgacgg cgtggatccc gcataacgac ggtaccaaca acttctatac
    45841 cgctaatctg ggtaacggca tcgtcgctat cggctataaa tctcagccgg tactggttca
    45901 gcctggtcag actggcgcga tgaacagcac cctgtgggtt ggcccggaaa tccaggacaa
    45961 aatggcagct gttgctccgc acctggatct gaccgttgat tacggttggt tgtggttcat
    46021 ctctcagccg ctgttcaaac tgctgaaatg gatccatagc tttgtgggta actggggctt
    46081 ctccattatc atcatcacct ttatcgttcg tggcatcatg tacccgctga ccaaagcgca
    46141 gtacacctcc atggcgaaga tgcgtatgct gcagccgaag attcaggcaa tgcgtgagcg
    46201 tctgggcgat gacaaacagc gtatcagtca ggaaatgatg gcgctgtaca aagctgagaa
    46261 ggttaacccg ctgggcggct gcttcccgct gctgatccag atgccaatct tcctggcgtt
    46321 gtactacatg ctgatgggtt ccgttgaact gcgtcaggca ccgtttgcac tgtggatcca
    46381 cgacctgtcg gcacaggacc cgtactacat cctgccgatc ctgatgggcg taacgatgtt
    46441 cttcattcag aagatgtcgc cgaccactgt gaccgacccg atgcagcaga agatcatgac
    46501 ctttatgccg gtcatcttca ccgtgttctt cctgtggttc ccgtcaggtc tggtactgta
    46561 ctatatcgtc agcaacctgg taaccattat tcagcagcag ctgatttacc gtggtctgga
    46621 aaaacgtggc ctgcatagcc gcgagaagaa aaaatcctga ttcggtgagt tttcgctaaa
    46681 ataagggcgg tcagttgacc gccttttttc ttttcgtagg gcggataagc accgcgcatc
    46741 cgccacacaa agcaacagga acatcatgag cgataatgac actatcgtag cccaggccac
    46801 gcctccggga cgtggcggcg ttggcatcct gcgcatctcc ggcttcaaag cccgtgaagt
    46861 tgccgaaacc gtgctgggta aactgcctaa gccgcgctac gccgattatc ttccgtttaa
    46921 agacgccgac ggcagcgtgc tcgatcaggg gattgcgcta tggttccctg gcccgaactc
    46981 gttcaccggc gaagatgtgc tggaactgca aggtcatggc ggtccggtga tcctcgacct
    47041 gctgttaaaa cgcattctga ccattcccgg cctgcggatt gctcgccctg gtgagttttc
    47101 cgaacgcgcg tttcttaacg ataaacttga cttagcccag gccgaggcga ttgccgatct
    47161 tatcgacgcc agttcggaac aggcggcccg ttcggcactt aactcgctgc aaggcgcatt
    47221 ctccgcacgg gttaatcatc tggtagaagc cctcacccac ttgcgcattt acgtcgaagc
    47281 ggcaattgat ttccctgatg aagagatcga tttcctctcc gacggcaaaa ttgaagccca
    47341 gcttaatgac gtgatggccg atctcgatgc agtgcgtgct gaagcacgtc agggtagttt
    47401 gttgcgcgaa gggatgaaag tagtgattgc cggacgtcct aatgccggta aatcgagcct
    47461 gttaaacgcg ctggcgggcc gtgaagcggc aatcgtaacc gatatcgccg gaaccacgcg
    47521 tgacgtgctg cgtgagcata tccacattga cggaatgccg ctgcatatca tcgacactgc
    47581 cgggctacgt gaggccagtg acgaagtgga acgtattggt atcgagcgcg cgtggcagga
    47641 aattgaacag gccgaccgcg tgctgtttat ggtcgatggc accacgacag acgccgttga
    47701 tccggcagag atctggccgg aatttatcgc ccgtctgccc gcgaaactgc cgatcaccgt
    47761 ggtacgcaat aaagccgata tcaccggcga aacgctggga atgagtgaag tgaacggtca
    47821 cgcgttaatt cgtctctcgg caaggactgg tgaaggcgtg gacgtgctgc gtaaccatct
    47881 caaacagagc atgggctttg acaccaatat ggaaggcggc ttcctggcgc gccgtcgcca
    47941 cttacaggcg ctggaacagg cagcggagca tttgcagcaa ggtaaagcgc aactgttggg
    48001 agcctgggca ggtgaactgc tggcggaaga gttgcgcctg gcacagcaga acttaagcga
    48061 aatcaccggg gaatttactt cagacgacct gctggggcgg attttctcca gcttctgtat
    48121 tggtaagtaa ccgcgcttac gaagccgtat tctgactgtc agatgcggct tcgcttcatt
    48181 gttagcactc ctgttatgcc tcaacccttt tttaaacatt aaaattctta cgtaatttat
    48241 aatctttaaa aaaagcattt aatactgctc cccgaacgat tgtgattcga ttcacattta
    48301 aacaatttca g
//
LOCUS       DAJTRC010000021        47487 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.021, whole genome shotgun sequence.
ACCESSION   DAJTRC010000021 DAJTRC010000000
VERSION     DAJTRC010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47487)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47487)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47487
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.021"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO357_001293"
     CDS             complement(365..982)
                     /gene="tdk"
                     /locus_tag="OO357_001293"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HCR5886299.1"
                     /translation="MAQLYFYYSAMNAGKSTALLQSSYNYQERGMRTVVYTAEIDDRF
                     GAGKVSSRIGLSSPAKLFNQNSSLFDEIRAEHEQQAIHCVLVDECQFLTRQQVYELSE
                     VVDQLDIPVLCYGLRTDFRGELFIGSQYLLAWSDKLVELKTICFCGRKASMVLRLDQA
                     GRPYNEGEQVVIGGNERYVSVCRKHYKEALQVGSLTAIQERHRHD"
     gene            1587..2000
                     /gene="hns"
                     /locus_tag="OO357_001294"
     CDS             1587..2000
                     /gene="hns"
                     /locus_tag="OO357_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator H-NS"
                     /protein_id="HCR5886300.1"
                     /translation="MSEALKILNNIRTLRAQARECTLETLEEMLEKLEVVVNERREEE
                     SAAAAEVEERTRKLQQYREMLIADGIDPNELLNSLAAVKSGTKAKRAQRPAKYSYVDE
                     NGETKTWTGQGRTPAVIKKAMDEQGKSLDDFLIKQ"
     gene            complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO357_001295"
     CDS             complement(2144..3052)
                     /gene="galU"
                     /locus_tag="OO357_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HCR5886301.1"
                     /translation="MAAINTKVKKAVIPVAGLGTRMLPATKAIPKEMLPLVDKPLIQY
                     VVNECIAAGITEIVLVTHSSKNSIENHFDTSFELEAMLEKRVKRQLLDEVQSICPPHV
                     TIMQVRQGLAKGLGHAVLCAHPVVGDEPVAVILPDVILDEYESDLSQDNLAEMIRRFD
                     ETGHSQIMVEPVADVTAYGVVDCKGVELAPGESVPMVGVVEKPKADVAPSNLAIVGRY
                     VLSADIWPLLAKTPPGAGDEIQLTDAIDMLIEKETVEAYHMKGKSHDCGNKLGYMQAF
                     VEYGIRHNTLGTEFKAWLEEEMGIKK"
     gene            complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO357_001296"
     CDS             complement(3254..4267)
                     /gene="rssB"
                     /locus_tag="OO357_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RssB"
                     /protein_id="HCR5886302.1"
                     /translation="MTQPLVGKQILIVEDEQVFRSLLDSWFSSLGATTVLAADGVDAL
                     ELLGGFTPDLMICDIAMPRMNGLKLLEHIRNRGDQTPVLVISATENMADIAKALRLGV
                     EDVLLKPVKDLNRLREMVFACLYPSMFNSRVEEEERLFRDWDAMVDNPAAAAKLLQEL
                     QPPVQQVISHCRVNYRQLVAADKPGLVLDIAALSENDLAFYCLDVTRAGHNGVLAALL
                     LRALFNGLLQEQLAHQNQRLPELGALLKQVNHLLRQANLPGQFPLLVGYYHRELKNLI
                     LVSAGLNATLNTGEHQVQISNGVPLGTLGNAYLNQLSQRCDAWQCQIWGTGGRLRLML
                     SAE"
     gene            complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO357_001297"
     CDS             complement(4359..5264)
                     /gene="rssA"
                     /locus_tag="OO357_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707140.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase RssA"
                     /protein_id="HCR5886303.1"
                     /translation="MRKIKIGLALGSGAARGWSHIGVINALKKVGIEIDIVAGCSIGS
                     LVGAAYACDRLSALEDWVTSFSYWDVLRLMDLSWQRGGLLRGERVFNQYREIMPETEI
                     ENCSRRFAAVATNLSTGRELWFTEGDLHLAIRASCSIPGLMAPVAHNGYWLVDGAVVN
                     PIPISLTRALGADIVIAVDLQHDAHLMQQDLLSFNVSEENSENGDSLPWHARLKERLG
                     SITTRRAVTAPTATEIMTTSIQVLENRLKRNRMAGDPPDILIQPVCPQISTLDFHRAH
                     AAIAAGQLAVERKMDELLPLVRTNI"
     gene            5377..5835
                     /locus_tag="OO357_001298"
     CDS             5377..5835
                     /locus_tag="OO357_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707139.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YchJ family protein"
                     /protein_id="HCR5886304.1"
                     /translation="MSQLCPCGSAVEYSLCCHPYVSGEKVAPDPEHLMRSRYCAFVMQ
                     DADYLIKTWHPSCGAAALRAELMTGFAHTEWLGLTVFEHCWQDADNIGFVSFVARFTE
                     GGKTGAIIERSRFLKENGQWYYIDGTRPQFGRNDPCPCGSGKKFKKCCGQ"
     gene            5885..6727
                     /gene="purU"
                     /locus_tag="OO357_001299"
     CDS             5885..6727
                     /gene="purU"
                     /locus_tag="OO357_001299"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HCR5886305.1"
                     /translation="MHSLQRKVLRTICPDQKGLIARITNICYKHELNIVQNNEFVDHR
                     TGRFFMRTELEGIFNDSTLLADLDSALPEGSVRELNPAGRRRIVILVTKEAHCLGDLL
                     MKANYGGLDVEIAAVIGNHDTLRSLVERFDIPFELVSHEGLSRNEHDQKMADAIDAYQ
                     PDYVVLAKYMRVLTPEFVARFPNKIINIHHSFLPAFIGARPYHQAYERGVKIIGATAH
                     YVNDNLDEGPIIMQDVIHVDHTYTAEDMMRAGRDVEKNVLSRALYKVLAQRVFVYGNR
                     TIIL"
     gene            6887..6971
                     /locus_tag="OO357_001300"
     tRNA            6887..6971
                     /locus_tag="OO357_001300"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6921..6923,aa:Tyr,seq:gta)
     gene            7018..7149
                     /locus_tag="OO357_001301"
     ncRNA           7018..7149
                     /ncRNA_class="other"
                     /locus_tag="OO357_001301"
                     /product="RtT sRNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00391"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="rtT sRNA, processed from tyrT transcript; Derived
                     by automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00391"
     gene            complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO357_001302"
     CDS             complement(7333..8010)
                     /gene="narI"
                     /locus_tag="OO357_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HCR5886306.1"
                     /translation="MQFLNMFFFDIYPYIAGAVFLIGSWLRYDYGQYTWRAASSQMLD
                     RKGMNLASNLFHIGILGIFVGHFFGMLTPHWMYEAWLPIEVKQKMAMFAGGASGVLCL
                     IGGVLLLKRRMFSPRVRATTTGADILILSLLVIQCALGLLTIPFSAQHMDGSEMMKLV
                     GWAQSVVTFHGGASQHLDGVAFIFRLHLVLGMTLFLLFPFSRLVHIWSVPVEYLTRKY
                     QLVRARH"
     gene            complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO357_001303"
     CDS             complement(8010..8720)
                     /gene="narJ"
                     /locus_tag="OO357_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase molybdenum cofactor assembly
                     chaperone"
                     /protein_id="HCR5886307.1"
                     /translation="MIELVIVSRLLEYPDAALWQHQQEMFEAIAASKNLSKEDAHALG
                     IFLRDLTAMDPLDAQVQYSELFDRGRATSLLLFEHVHGESRDRGQAMVDLLAQYEQHG
                     LQLNSRELPDHLPLYLEYLSQLPQSEAVEGLKDIAPILALLSARLQQRESRYAVMFDL
                     LLKLANTAIDSDKVAEKIADEARDDTPQALDAVWEEEQVKFFADKGCGDSAITAHQRR
                     FAGAVAPQYLNITTGGQH"
     gene            complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO357_001304"
     CDS             complement(8717..10255)
                     /gene="narH"
                     /locus_tag="OO357_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5886308.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCKNVWTSREGVEYAWFNNV
                     ETKPGQGFPTDWENQEKYKGGWIRKINGKLQPRMGNRAMLLGKIFANPHLPGIDDYYE
                     PFDFDYQNLHTAPEGSKSQPIARPRSLITGERMAKIEKGPNWEDDLGGEFDKLAKDKN
                     FDNIQKAMYSQFENTFMMYLPRLCEHCLNPACVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRMCITGCPYKKIYFNWKSGKSEKCIFCYPRIEAGQPTVCSETCVGRIRYLGVLLYD
                     ADAIERAASTENEKDLYQRQLDVFLDPNDPKVIEQAIKDGIPLSVIEAAQQSPVYKMA
                     MEWKLALPLHPEYRTLPMVWYVPPLSPIQSAADAGELGSNGILPDVESLRIPVQYLAN
                     LLTAGDTKPVLRALKRMLAMRHYKRAETVDGKVDTRALEEVGLTEAQAQEMYRYLAIA
                     NYEDRFVVPSSHRELAREAFPEKNGCGFTFGDGCHGSDTKFNLFNSRRIDAIDVTSKT
                     EPHP"
     gene            complement(10252..13995)
                     /locus_tag="OO357_001305"
     CDS             complement(10252..13995)
                     /locus_tag="OO357_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit alpha"
                     /protein_id="HCR5886309.1"
                     /translation="MSKFLDRFRYFKQKGETFADGHGQLLNTNRDWEDGYRQRWQHDK
                     IVRSTHGVNCTGSCSWKIYVKNGLVTWETQQTDYPRTRPDLPNHEPRGCPRGASYSWY
                     LYSANRLKYPMMRKRLMKMWREAKALHSDPVEAWASIIEDADKAKSFKQARGRGGFVR
                     SSWQEVNELIAASNVYTIKNYGPDRVAGFSPIPAMSMVSYASGARYLSLIGGTCLSFY
                     DWYCDLPPASPQTWGEQTDVPESADWYNSSYIIAWGSNVPQTRTPDAHFFTEVRYKGT
                     KTVAVTPDYAEIAKLCDLWLAPKQGTDAAMALAMGHVMLREFHLDNPSQYFTDYVRRY
                     TDMPMLVMLEERDGYYAAGRMLRAADLVDALGQENNPEWKTVAFNTNGEMVAPNGSIG
                     FRWGEKGKWNLEQRDGKTGEETELQLSLLGSQDEIAEVGFPYFGGDGTEHFNKVELEN
                     VLLHKLPVKRLQLADGSTALVTTVYDLTLANYGLERGLNDVNCATSYDDVKAYTPAWA
                     EQITGVSRSQIIRIAREFADNADKTHGRSMIIVGAGLNHWYHLDMNYRGLINMLIFCG
                     CVGQSGGGWAHYVGQEKLRPQTGWQPLAFALDWQRPARHMNSTSYFYNHSSQWRYETV
                     TAEELLSPMADKSRYTGHLIDFNVRAERMGWLPSAPQLGTNPLTIAGEAEKAGMNPVD
                     YTVKSLKEGSIRFAAEQPENGKNHPRNLFIWRSNLLGSSGKGHEFMLKYLLGTEHGIQ
                     GKDLGQQGGVKPEEVDWQDNGLEGKLDLVVTLDFRLSSTCLYSDIILPTATWYEKDDM
                     NTSDMHPFIHPLSAAVDPAWEAKSDWEIYKAIAKKFSEVCVGYLGKETDIVTLPIQHD
                     SAAELAQPLDVKDWKKGECDLIPGKTAPHIMVVERDYPATYERFTSIGPLMEKIGNGG
                     KGIAWNTQSEMDLLRKLNYTKAEGPAKGQPMLNTAIDAAEMILTLAPETNGQVAVKAW
                     AALSEFTGRDHTHLALNKEDEKIRFRDIQAQPRKIISSPTWSGLEDEHVSYNAGYTNV
                     HELIPWRTLSGRQQLYQDHQWMRDFGESLLVYRPPIDTRSVKEVIGQKSNGNPEKALN
                     FLTPHQKWGIHSTYSDNLLMLTLGRGGPVVWLSEADAKDLGIADNDWIEVFNSNGALT
                     ARAVVSQRVPAGMTMMYHAQERIVNLPGSEITQQRGGIHNSVTRITPKPTHMIGGYAH
                     LAYGFNYYGTVGSNRDEFVVVRKMKNIDWLDGEGNDQVQESVK"
     gene            complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO357_001306"
     CDS             complement(14388..15779)
                     /gene="narK"
                     /locus_tag="OO357_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate transporter NarK"
                     /protein_id="HCR5886310.1"
                     /translation="MSHSSASERATGAVITDWRPEDPAFWQQRGQRIASRNLWISVPC
                     LLLAFCVWMLFSAVAVNLPKVGFNFTTDQLFMLTALPSVSGALLRVPYSFMVPIFGGR
                     RWTAFSTGILIIPCVWLGFAVQDTSTPYSVFIIISLLCGFAGANFASSMANISFFFPK
                     QKQGGALGLNGGLGNMGVSVMQLVAPLVVSLSIFAVFGSQGVKQPDGTELYLANASWI
                     WVPFLAIFTIAAWFGMNDLATSKASIKEQLPVLKRGHLWIMSLLYLATFGSFIGFSAG
                     FAMLSKTQFPDVQILQYAFFGPFIGALARSAGGALSDRLGGTRVTLVNFILMAIFSGL
                     LFLTLPTDGQGGSFMAFFAVFLALFLTAGLGSGSTFQMISVIFRKLTMDRVKAEGGSD
                     ERAMREAATGTAAALGFISAIGAIGGFFIPKAFGSSLALTGSPVGAMKVFLIFYIACV
                     VITWAVYGRHSKK"
     gene            16118..17914
                     /gene="narX"
                     /locus_tag="OO357_001307"
     CDS             16118..17914
                     /gene="narX"
                     /locus_tag="OO357_001307"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite two-component system sensor
                     histidine kinase NarX"
                     /protein_id="HCR5886311.1"
                     /translation="MLKRCLSPLTLVNQVALIVLLSTAIGLAGMAVSGWLVQGVQGSA
                     HAINKAGSLRMQSYRLLAAVPLSEKDKPLIKEMEQTAFSAELTRAAERDGQLAQLQGL
                     QDYWRNELIPALMRAQNRETVSADVSQFVAGLDQLVSGFDRTTEMRIETVVLVHRVMA
                     VFMALLLVFTIIWLRARLLQPWRQLLAMASAVSHRDFTQRANISGRNEMAMLGTALNN
                     MSAELAESYAVLEQRVQEKTAGLEHKNQILSFLWQANRRLHSRAPLCERLSPVLNGLQ
                     NLTLLRDIELRVYDTDDEENHQEFTCQPDMTCDDKGCQLCPRGVLPVGDRGTTLKWRL
                     ADSHTQYGILLATLPQGRHLSHDQQQLVDTLVEQLTATLALDRHQERQQQLIVMEERA
                     TIARELHDSIAQSLSCMKMQVSCLQMQGDALPESSRELLSQIRNELNASWAQLRELLT
                     TFRLQLTEPGLRPALEASCEEYSAKFGFPVKLDYQLPPRLVPSHQAIHLLQIAREALS
                     NALKHSQASEVVVTVAQNDNQVKLTVQDNGCGVPENAIRSNHYGMIIMRDRAQSLRGD
                     CRVRRRESGGTEVVVTFIPEKTFTDVQGDTHE"
     gene            17907..18557
                     /gene="narL"
                     /locus_tag="OO357_001308"
     CDS             17907..18557
                     /gene="narL"
                     /locus_tag="OO357_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator NarL"
                     /protein_id="HCR5886312.1"
                     /translation="MSNQEPATILLIDDHPMLRTGVKQLISMAPDITVVGEASNGEQG
                     IELAESLDPDLILLDLNMPGMNGLETLDKLREKSLSGRIVVFSVSNHEEDVVTALKRG
                     ADGYLLKDMEPEDLLKALHQAAAGEMVLSEALTPVLAASLRANRATTERDVNQLTPRE
                     RDILKLIAQGLPNKMIARRLDITESTVKVHVKHMLKKMKLKSRVEAAVWVHQERIF"
     gene            complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO357_001309"
     CDS             complement(18558..19952)
                     /gene="ychO"
                     /locus_tag="OO357_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inverse autotransporter invasin YchO"
                     /protein_id="HCR5886313.1"
                     /translation="MSRFVPRIIPFYLLLLVAGGTANAQSTFEQKAANPFDNNNDGLP
                     DLGMAPENHDGEKHFAEIVKDFGETSMNDNGLDTGEQAKAFALGKVRDALSQQVNQHV
                     ESWLSPWGNASVDVKVDNEGHFTGSRGSWFVPLQDNDRYLTWSQLGLTQQDNGLVSNV
                     GVGQRWARGNWLVGYNTFYDNLQDENLQRAGFGAEAWGEYLRLSANFYQPFAAWHEQT
                     ATQEQRMARGYDLTARMRMPFYQHLNTSVSLEQYFGDRVDLFNSGTGYHNPVALSLGL
                     NYTPVPLVTVTAQHKQGESGENQNNLGLNLNYRFGVPLKKQLSAGEVAESQSLRGSRY
                     DNPQRNNLPTLEYRQRKTLTVFLATPPWDLKPGETVPLKLQIRSRYGIRQLIWQGDTQ
                     ILSLTPGAQANSAEGWTLIMPDWQNGEGASNHWRLSVVVEDNQGQRVSSNEITLTLVE
                     PFDALSNDELRWEP"
     gene            20137..20490
                     /gene="ychN"
                     /locus_tag="OO357_001310"
     CDS             20137..20490
                     /gene="ychN"
                     /locus_tag="OO357_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/F sulfur relay family protein YchN"
                     /protein_id="HCR5886314.1"
                     /translation="MQKIVIVANGAPYGSESLFNSLRLAIALREQESNLDLRLFLMSD
                     AVTAGLRGQKPGEGYNIQQMLEILTAQNVPVKLCKTCTDGRGISTLPLIDGVEIGTLV
                     ELAQWTLSADKVLTF"
     gene            complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO357_001311"
     CDS             complement(20534..21229)
                     /gene="chaC"
                     /locus_tag="OO357_001311"
                     /EC_number="4.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-specific
                     gamma-glutamylcyclotransferase"
                     /protein_id="HCR5886315.1"
                     /translation="MITRDFLMNADCKTAFGAIEESLLWSAEQRAASLAATLACRPDE
                     GPVWIFGYGSLMWNPALEFTESCTGTLVGWHRAFCLRLTAGRGTAHQPGRMLALKEGG
                     RTTGVAYRLPEETLEQELTLLWKREMITGCYLPTWCQLDLDDGRTVNAIVFIMDPRHP
                     EYESDTRAQVIAPLIAAASGPLGTNAQYLFSLEQELIKLGMQDDGLNDLLVSVKKLLA
                     ENYPDGVLRPGFA"
     gene            complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO357_001312"
     CDS             complement(21387..21617)
                     /gene="chaB"
                     /locus_tag="OO357_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HCR5886316.1"
                     /translation="MPYKTKSDLPESVKHVLPSHAQDIYKEAFNSAWDQYKDKEDRRD
                     DASREETAHKVAWAAVKHEYAKGDDDKWHKKS"
     gene            21887..22987
                     /gene="chaA"
                     /locus_tag="OO357_001313"
     CDS             21887..22987
                     /gene="chaA"
                     /locus_tag="OO357_001313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-potassium/proton antiporter ChaA"
                     /protein_id="HCR5886317.1"
                     /translation="MSNAQEAVKTRHKETSLIFPVLVLVVLFLWGSSQTLPVVIAINL
                     LALIGILSSAFSVVRHADVLAHRLGEPYGSLILSLSVVILEVSLISALMATGDAAPTL
                     MRDTLYSIIMIVTGGLVGFSLLLGGRKFATQYMNLFGIKQYLIALFPLAIIVLVFPMA
                     LPAANFSTGQALLVALISAAMYGVFLLIQAKTHQSLFVYEHEDDSDDDDPHHGKTSAH
                     SSLWHAIWLIIHLIAVIAVTKMNASSLETLLDSMNAPVAFTGFLVALLILSPEGLGAL
                     KAVLNNQVQRAMNLFFGSVLATISLTVPVVTLIAFMTGNELQFALGAPEMVVMVASLV
                     LCHISFSTGRTNVLNGAAHLALFAAYLMTIFA"
     gene            23392..23499
                     /locus_tag="OO357_001314"
     CDS             23392..23499
                     /locus_tag="OO357_001314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin Ldr family
                     protein"
                     /protein_id="HCR5886318.1"
                     /translation="MTLAQFAMTFWHDLAAPILAGIIAAAIVSWWRNRK"
     gene            complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO357_001315"
     CDS             complement(23648..24502)
                     /gene="kdsA"
                     /locus_tag="OO357_001315"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HCR5886319.1"
                     /translation="MKQKVVSIGDINVANDLPFVLFGGMNVLESRDLAMRICEHYVTV
                     TQKLGIPYVFKASFDKANRSSIHSYRGPGLEEGMKIFQELKQTFGVKIITDVHEPSQA
                     QPVADVVDVIQLPAFLARQTDLVEAMAKTGAVINVKKPQFVSPGQMGNIVDKFKEGGN
                     EKVILCDRGANFGYDNLVVDMLGFSIMKKVSGNSPVIFDVTHALQCRDPFGAASGGRR
                     AQVAELARAGMAVGLAGLFIEAHPDPEHAKCDGPSALPLAKLEPFLKQMKAIDDLVKG
                     FEELDTSK"
     gene            complement(24538..25347)
                     /locus_tag="OO357_001316"
     CDS             complement(24538..25347)
                     /locus_tag="OO357_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing protein"
                     /protein_id="HCR5886320.1"
                     /translation="MRSLADFEFNKAPLCEGMILACEAIRRDFPSQDVYDELERLVSL
                     AKEEISQLLPLEEQLEKLIALFYGDWGFKASRGVYRLSDALWLDQVLKNRQGSAVSLG
                     AVLLWVANRLDLPLLPVIFPTQLILRIECPDGEIWLINPFNGESLSEHMLDGWLKGNI
                     SPSAELFYEDLDEADNIEVIRKLLDTLKASLMEENQMELALRTSEALLQFNPEDPYEI
                     RDRGLIYAQLDCEHVALNDLSYFVERCPEDPISEMIRAQINNIAHKHIVLH"
     gene            complement(25351..25743)
                     /locus_tag="OO357_001317"
     CDS             complement(25351..25743)
                     /locus_tag="OO357_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SirB family protein"
                     /protein_id="HCR5886321.1"
                     /translation="MTSFSTLLSVHLISIALSVGLLTLRFWLRYQKHPQAFARWTRIV
                     PPVVDTVLLLSGIALMAKAHILPFSGQAQWLTEKLFGVIIYIVLGFIALDYRRMHSQQ
                     ARIIAFPLALVVLYIIIKLATTKVPLLG"
     gene            complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO357_001318"
     CDS             complement(25740..26573)
                     /gene="prmC"
                     /locus_tag="OO357_001318"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HCR5886322.1"
                     /translation="MEYQHWLREAISQLQASESPRRDAEILLEHVTGRGRTFILAFGE
                     TQLTDEQCQQLDALLTRRRDGEPIAHLTGVREFWSLPLFVSPATLIPRPDTECLVEQA
                     LARLPEQPCRILDLGTGTGAIALALASERPDCEIIAVDRMPDAVSLAQRNAQHLAIKN
                     IHILQSDWFSALAGQQFAMIVSNPPYIDEQDPHLQQGDVRFEPLTALVAADSGMADIV
                     HIIEQSRNALVSGGFLLLEHGWQQGEAVRQAFILAGYHDVETCRDYGDNERVTLGRYY
                     Q"
     gene            complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO357_001319"
     CDS             complement(26573..27655)
                     /gene="prfA"
                     /locus_tag="OO357_001319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012132158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HCR5886323.1"
                     /translation="MKPSIVAKLEALHERHEEVQALLGDAQTIADQERFRALSREYAQ
                     LSDVSRCFTDWQQVQEDIETAQMMLDDPEMREMAQDELREAKEKSEQLEQQLQVLLLP
                     KDPDDERNAFLEVRAGTGGDEAALFAGDLFRMYSRYAEARRWRVEIMSASEGEHGGYK
                     EIIAKISGDGVYGRLKFESGGHRVQRVPATESQGRIHTSACTVAVMPELPDAELPDIN
                     PADLRIDTFRSSGAGGQHVNTTDSAIRITHLPTGIVVECQDERSQHKNKAKALSVLGA
                     RIHAAEMAKRQQAEASTRRNLLGSGDRSDRNRTYNFPQGRVTDHRINLTLYRLDEVME
                     GKLDMLIEPIIQEHQADQLAALSEQE"
     gene            complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO357_001320"
     CDS             complement(27697..28953)
                     /gene="hemA"
                     /locus_tag="OO357_001320"
                     /EC_number="1.2.1.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_041689438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl-tRNA reductase"
                     /protein_id="HCR5886324.1"
                     /translation="MTLLALGINHKTAPVSLRERVSFSPDKLDQALDSLLAQPMVQGG
                     VVLSTCNRTELYLSVEEQDNLQEALIRWLCDYHNLNEEDLRKSLYWHQDNDAVSHLMR
                     VASGLDSLVLGEPQILGQVKKAFVDSQKGHMKASELERMFQKSFSVAKRVRTETDIGA
                     SAVSVAFAACTLARQIFESLSTVTVLLVGAGETIELVARHLREHKVQKMIIANRTRER
                     AQILADEVGAEVIALSEIDERLREADIIISSTASPLPIIGKGMVERALKSRRNQPMLL
                     VDIAVPRDVEPEVGKLANAYLYSVDDLQSIISHNLAQRKAAAVEAETIVAQETSEFMA
                     WLRAQSASETIREYRSQAEHVRDELTAKALAALEQGGDAQAIMQDLAWKLTNRLIHAP
                     TKSLQQAARDGDNERLNILRDSLGLE"
     gene            29167..29790
                     /gene="lolB"
                     /locus_tag="OO357_001321"
     CDS             29167..29790
                     /gene="lolB"
                     /locus_tag="OO357_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein localization protein LolB"
                     /protein_id="HCR5886325.1"
                     /translation="MPLPDFRLIRLLPLAALVLTACSVTTPKGPGKSPDSPQWRQHQQ
                     DVRNLNQYQTRGAFAYISDQQKVYARFFWQQTGQDRYRLLLTNPLGSTELELNAQPGN
                     VQLVDNKGQRYTADDAEEMIGKLTGMPIPLNSLRQWILGLPGDATDYKLDDQYRLSEI
                     TYSQNGKNWKVVYGGYDTKTQPAMPANMELTDGGQRIKLKMDNWIVK"
     gene            29790..30641
                     /gene="ispE"
                     /locus_tag="OO357_001322"
     CDS             29790..30641
                     /gene="ispE"
                     /locus_tag="OO357_001322"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HCR5886326.1"
                     /translation="MRTQWPSPAKLNLFLYITGQRADGYHTLQTLFQFLDYGDTISIE
                     LRDDGDIRLLTPVEGVEHEDNLIVRAARLLMKTAADSGRLPTGSGANISIDKRLPMGG
                     GLGGGSSNAATVLVALNHLWQCGLSMDELAEMGLTLGADVPVFVRGHAAFAEGVGEIL
                     TPVDPPEKWYLVAHPGVSIPTPVIFKDPELPRNTPKRSIETLLKCEFSNDCEVIARKR
                     FREVDAVLSWLLEYAPSRLTGTGACVFAEFDTESEARQVLEQAPEWLNGFVAKGVNLS
                     PLHRAML"
     gene            30792..31739
                     /gene="prs"
                     /locus_tag="OO357_001323"
     CDS             30792..31739
                     /gene="prs"
                     /locus_tag="OO357_001323"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HCR5886327.1"
                     /translation="MPDMKLFAGNATPVLAQRIANRLYTSLGDAAVGRFSDGEVSVQI
                     NENVRGGDIFIIQSTCAPTNDNLMELVVMVDALRRASAGRITAVIPYFGYARQDRRVR
                     SARVPITAKVVADFLSSVGVDRVLTVDLHAEQIQGFFDVPVDNVFGSPILLEDMLQLN
                     LDNPIVVSPDIGGVVRARAIAKLLNDTDMAIIDKRRPRANVSQVMHIIGDVAGRDCVL
                     VDDMIDTGGTLCKAAEALKERGAKRVFAYATHPIFSGNAANNLRNSVIDEVVVCDTIP
                     LSDEIKSLPNVRTLTLSGMLAEAIRRISNEESISAMFEH"
     gene            31864..33543
                     /gene="dauA"
                     /locus_tag="OO357_001324"
     CDS             31864..33543
                     /gene="dauA"
                     /locus_tag="OO357_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylic acid transporter DauA"
                     /protein_id="HCR5886328.1"
                     /translation="MNKIFSSHVMPFRALIDVCWKEKYTAARFTRDLIAGITVGIIAI
                     PLAMALAIGSGVAPQYGLYTAAVAGIVIALTGGSRFSVSGPTAAFVVILYPVSQQFGL
                     AGLLVATLLSGIFLILMGLARFGRLIEYIPVSVTLGFTSGIGITIGTMQIKDFLGLQM
                     AHVPEHYLQKVGALFMALPTINVGDAAIGIVTLGILVFWPRLGIRLPGHLPALLAGCA
                     VMGIVNLLGGHVATIGSQFHYVLADGSQGNGIPQLLPQLVLPWDLPNSEFTLTWDSIR
                     TLLPAAFSMAMLGAIESLLCAVVLDGMTGTKHKANSELVGQGLGNIIAPFFGGITATA
                     AIARSAANVRAGATSPISAVIHSILVILALLVLAPLLSWLPLSAMAALLLMVAWNMSE
                     AHKVVDLLRHAPKDDIIVMLLCMSLTVLFDMVIAISVGIVLASLLFMRRIARMTRLAP
                     VVVDVPDDVLVLRVIGPLFFAAAEGLFTDLESRLEGKRIVILKWDAVPVLDAGGLDAF
                     QRFVKRLPEGCELRVCNVEFQPLRTMARAGIQPIPGRLAFFPNRRAAMADL"
     gene            complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO357_001325"
     CDS             complement(33598..33876)
                     /gene="ychH"
                     /locus_tag="OO357_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress-induced protein YchH"
                     /protein_id="HCR5886329.1"
                     /translation="MKRKNASILGNVLMGLGLVVMVVGVGYSILNQLPQFNMPQYFAH
                     GAVLSIFVGAILWLAGARVGGHEQVCDRYWWVRHYDKRCRRSDNRRHS"
     gene            34154..34738
                     /gene="pth"
                     /locus_tag="OO357_001326"
     CDS             34154..34738
                     /gene="pth"
                     /locus_tag="OO357_001326"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HCR5886330.1"
                     /translation="MTIKLIVGLANPGAEYAATRHNAGAWFVDLLAERLRAPLREEAK
                     FFGYTSRVTLGSEDVRLLVPTTFMNLSGKAVAAMASFFRINPDEILVAHDELDLPPGV
                     AKFKLGGGHGGHNGLKDIISKLGNNPNFHRLRIGIGHPGDKNKVVGFVLGKPPVSEQK
                     LIDEAIDEAARCTEMWFTDGLTKATNRLHAFKAQ"
     gene            34855..35946
                     /gene="ychF"
                     /locus_tag="OO357_001327"
     CDS             34855..35946
                     /gene="ychF"
                     /locus_tag="OO357_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HCR5886331.1"
                     /translation="MGFKCGIVGLPNVGKSTLFNALTKAGIEAANFPFCTIEPNTGVV
                     PMPDPRLDQLAEIVKPQRTLPTTMEFVDIAGLVKGASKGEGLGNQFLTNIRETEAIGH
                     VVRCFENDNIIHVSGKVNPADDIEVINTELALADLDTCERAIHRVQKKAKGGDKDAKA
                     ELAVLEKCLPQLENAGMLRALDLSAEEKAAIRYLSFLTLKPTMYIANVNEDGFENNPY
                     LDQVREIAAKEGSVVVPVCAAVEADIAELDDEERDEFMQELGLEEPGLNRVIRAGYKL
                     LNLQTYFTAGVKEVRAWTIPVGATAPQAAGKIHTDFEKGFIRAQTISFEDFITYKGEQ
                     GAKEAGKMRAEGKDYIVKDGDVMNFLFNV"
     gene            36790..39657
                     /locus_tag="OO357_001328"
     CDS             36790..39657
                     /locus_tag="OO357_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5886332.1"
                     /translation="MGIKQHNGNTKADRLAELKIRSPSIQLIKFGAIGLNAIIFSPLL
                     IAADTGSQYGTNITINDGDRITGDTADPSGNLYGVMTPAGNTPGNINLGNDVTVNVND
                     ASGYAKGIIIQGKNSSLTANRLTVDVVGQTSAIGINLIGDYTHADLGTGSTIKSNDDG
                     IIIGHSSTLTATQFTIENSNGIGLTINDYGTSVDLGSGSKIKTDGSTGVYIGGLNGNN
                     ANGAARFTATDLTIDVQGYSAMGINVQKNSVVDLGTNSTIKTNGDNAHGLWSFGQVSA
                     NALTVDVTGAAANGVEVRGGTTTIGADSHISSAQGGGLVTSSSDATINFSGTAAQRNS
                     IFSGGSYGASAQTATAVINMQNTDITVDRNGSLALGLWALSGGRITGDSLAITGAAGA
                     RGIYAMTNSQIDLTSDLVIDMSTPDQMAIATQHDDGYAASRINASGRMLINGSVLSKG
                     GLINLDMHPGSVWTGSSLSDNVNGGKLDVAMNNSVWNVTSNSNLDTLALSHSTVDFAS
                     HGSTAGTFTTLNVENLSGNSTFIMRADVVGEGNGVNNRGDLLNISGSSAGNHVLAIRN
                     QGSEATTGNEVLTVVKTTDGAASFSASSQVELGGYLYDVRKNGTNWELYASGTVPEPT
                     PNPEPTPAPAQPPIVNPDPTPEPAPTPKPTTTADAGGNYLNVGYLLNYVENRTLMQRM
                     GDLRNQSKDGNIWLRSYGGSLDSFASGKLSGFDMGYSGIQFGGDKRLSDVMPLYVGLY
                     IDSTHASPDYSGGDGTARSDYMGMYASYMAQNGFYSDLVIKASRQKNSFHVLDSQNNG
                     VNANGTANGMSISLEAGQRFNLSPTGYGFYIEPQTQLTYSHQNEMAMKASNGLNIHLN
                     HYESLLGRASMILGYDITAGNSQLNVYVKTGAIREFSGDTEYLLNDSREKYSFKGNGW
                     NNGVGVSAQYNKQHTFYLEADYTQGNLFDQKQVNGGYRFSF"
     gene            complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO357_001329"
     CDS             complement(39757..41676)
                     /gene="dhaR"
                     /locus_tag="OO357_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415719.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase operon
                     transcriptional regulator DhaR"
                     /protein_id="HCR5886333.1"
                     /translation="MSGAFNNDGRGISPLIATSWERCNKLMKRETWNVPHQAQGVTFA
                     SIYRRKKAMLTLGQAALEDAWEYMAPRECALFILDETACILSRNGDPQTLQQLSALGF
                     NDGTYCAEGIIGTCALSLAAISGQAVKTMADQHFKQALWNWAFCATPLFDSKGRLTGT
                     IALACPVEQTTAADLPLTLAIAREVGNLLLTDSLLAETNRHLNQLNALLESMDDGVIS
                     WDEQGNLQFINAQAARVLRLDATASQGRAITELLTLPAVLQQAIKLAHPLKHVEATFE
                     SQHQFIDAVITLKPIIETQGTSFILLLHPVEQMRQLMTSQLGKVSHTFAHMPQDDPQT
                     RRLIHFGRQAARSSFPVLLCGEEGVGKALLSQAIHNESERAAGPYIAVNCELYGDAAL
                     AEEFIGGDRTDNENGRLSRLELAHGGTLFLEKIEYLAVELQSALLQVIKQGVITRLDA
                     RRLIPIDVKVIATTTADLAMLVEQNRFSRQLYYALHAFEITIPPLRMRRGSIPALVNN
                     KLRSLEKRFSTRLKIDDDALARLVSCAWPGNDFELYSVIENLALSSDNGRIRVSDLPE
                     HLFTEQATDDVSATRLSTSLSFAEVEKEAIINAAQVTGGRIQEMSALLGIGRTTLWRK
                     MKQHGIDAGQFKRRV"
     gene            41904..42974
                     /gene="dhaK"
                     /locus_tag="OO357_001330"
     CDS             41904..42974
                     /gene="dhaK"
                     /locus_tag="OO357_001330"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HCR5886334.1"
                     /translation="MKKLINDVQDVLDEQLAGLAKAHPSLTLHQDPVYVTRADAPVAG
                     KVALLSGGGSGHEPMHCGYIGQGMLSGACPGEIFTSPTPDKIFECSMQIDGGEGVLLI
                     IKNYTGDILNFETATELLHDSGVKVTTVVIDDDVAVKDSLYTAGRRGVANTVLIEKLV
                     GAAAERGDSLDACAELGRKLNNQGHSIGIALGACTVPAAGKPSFTLADNEMEFGVGIH
                     GEPGIDRRPFSSLDQTVDEMFDTLLEHGSYHRTLRFWDYQQGSWQEEPQTKQPLQSGD
                     RVIALVNNLGATPLSELYGVYNRLTTRCQQAGLTIERNLIGAYCTSLDMTGFSITLLK
                     VDDETLALWDAPVHTPALNWGK"
     gene            42985..43617
                     /gene="dhaL"
                     /locus_tag="OO357_001331"
     CDS             42985..43617
                     /gene="dhaL"
                     /locus_tag="OO357_001331"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707108.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase ADP-binding subunit
                     DhaL"
                     /protein_id="HCR5886335.1"
                     /translation="MSLSRTQIVNWLTRCGDIFNTESEYLTGLDREIGDADHGLNMNR
                     GFSKVVEKLPAIADKDIGFILKNTGMTLLSSVGGASGPLFGTFFIRAAQATQARQSLT
                     LEELYQMFRDGADGVISRGKAEPGDKTMCDVWVPVVESLRQSSEQNLSVPVALEAASS
                     IAESAAQSTITMQARKGRASYLGERSIGHQDPGATSVMFMMQMLALAAKE"
     gene            43628..45046
                     /gene="dhaM"
                     /locus_tag="OO357_001332"
     CDS             43628..45046
                     /gene="dhaM"
                     /locus_tag="OO357_001332"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaM"
                     /protein_id="HCR5886336.1"
                     /translation="MVNLVIVSHSSRLGEGVGELARQMLMSDSCKIAIAAGIDDPQNP
                     IGTDAVKVMEAIESVADADHVLVMMDMGSALLSAETALELLAPEIAAKVRLCAAPLVE
                     GTLAATVSAASGADIDKVIFDAMHALEAKREQLGLPSSDTEISDTCPPYDEEARSLAV
                     VIKNRNGLHVRPASRLVYTLSTFNADMLLEKNGKCVTPESINQIALLQVRYNDTLRLI
                     AKGPEAEEALIAFRHLAEDNFGETEEVAPPTLRPVLPVSGKAFYYQPVLCTVQAKSTL
                     TAEEEQDRLRQAIDFTLLDLMTLTAKAEASGLDDIAAIFSGHHTLLGDPELLAAASEL
                     LQHEHCTAEYAWQQVLKELSQQYQQLDDEYLQARYIDVDDLLHRTLVHLTQTKEELPQ
                     FNSPTILLAENIYPSTVLQLDPAVVKGICLSAGSPVSHSALIARELGIGWICQQGEKL
                     YAIQPEETLTLDVKTQRFNRQG"
     gene            45366..47063
                     /gene="treA"
                     /locus_tag="OO357_001333"
     CDS             45366..47063
                     /gene="treA"
                     /locus_tag="OO357_001333"
                     /EC_number="3.2.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase"
                     /protein_id="HCR5886337.1"
                     /translation="MKSPAPSRPQKMALIPACIFLCFAALSVQAEETPVTPQPPDILL
                     GPLFNDVQNVKLFPDQKTFADAVPNSDPLMILADYRMQQNQSGFDLRHFVNVNFTLPK
                     EGEKYVPPEGQSLREHIDGLWPVLTRSTENTEKWDSLLPLPEPYVVPGGRFREVYYWD
                     NYFTMLGLAESGHWDKIADMVANFAHEINTYGHIPNGNRSYYLSRSQPPFFALMVELL
                     AQHEGDAALKQYLPQMQKEYAYWMDGVENLQAGQQEKRVVKLQDSTLLNRYWDDRDTP
                     RPESWVEDIATAKSNPNRPATEIYRDLRSAAASGWDFSSRWMDNPQQLNTLRTTSIVP
                     VDLNSLMFKMEKILARASKAAGDNAMANQYETLANARQKGIEKYLWNDQQGWYADYDL
                     KSHKVRNQLTAAALFPLYVNAAAKDRANKMATATKTHLLQPGGLNTTSVKSGQQWDAP
                     NGWAPLQWVATEGLQNYGQKEVAMDISWHFLTNVQHTYDREKKLVEKYDVSTTGTGGG
                     GGEYPLQDGFGWTNGVTLKMLDLICPKEQPCDNVPATHPTVKSATTQPSTKEAQPTP"
     gene            complement(47142..47437)
                     /locus_tag="OO357_001334"
                     /pseudo
     CDS             complement(47142..47437)
                     /locus_tag="OO357_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001367998.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT        11912 a        12240 c        11641 g        11694 t
ORIGIN      
        1 aagggccttc ccgagagtgg taaaggagag gccctggaaa taccccacag ctccgaaaaa
       61 gagccagtca gttgcttaac tgactggcat taccccttct ggggccgttt ttttgtttac
      121 ccaaagcaac ttttccataa accgacagca ttagccttca tcatatttgc gacgatgtat
      181 aacgcctaaa cacagggata ttgtacttta caggtcacaa gtcaacgtcg gtgcttaaga
      241 gccctgtgag gcgtatagcg gcgttaaaaa actgctgaga agggtatata gcccggaaga
      301 agtgcgtaaa acgaactgac aggataaaag tgccctgctc accctgtcag taaagaaatt
      361 cttattaatc gtggcgatgc ctttcctgaa tagccgttaa tgagccgact tgtaacgcct
      421 ctttatagtg tttacggcat acagaaacgt atcgttcatt accaccaatt accacctgct
      481 caccttcgtt ataaggtctg cctgcttgat caagacgcag caccatgctt gctttacggc
      541 cacaaaaaca gatggttttt aattcaacca gtttgtcgga ccatgccagt aagtattggc
      601 tgccaataaa taattcacct cgaaaatcgg tacgtaaacc ataacaaagt acgggtatat
      661 cgagttgatc gacaacctcc gataattcat atacttgttg tctggttaaa aactggcatt
      721 catcaaccag tacgcaatga attgcctgct gttcatgttc cgcacgaatc tcatcaaata
      781 atgatgaatt ttggttaaat aattttgcag gcgatgacaa acctatacgc gaactgactt
      841 tcccggcacc aaagcgatca tcaatttctg ccgtatatac gacagtgcgc atgccgcgtt
      901 cctggtaatt gtatgaagat tgcaacaatg ctgtagactt acccgcattc attgcggaat
      961 agtagaaata tagctgtgcc atcagccaca ggccctcaat gatatgaaat aaagttggct
     1021 ggagtttatc ataattcgtc agtttttcag ctcaacagta tgcacagaag tgggtatatg
     1081 cgttctccct tacgaagcct tgcataatcc ttctgagcta tcattacaac tgcctcgctt
     1141 gttataagcg ggtaaatgac tgctggtaac tattcacaat ctttaacctg ttgcgcaagt
     1201 aatagccctc tgttgacctc caggagatag tgcaatacta agtccatgct cttattgcga
     1261 ctgttctact tttcatcatt cgcttaatag ggaattctcg taaacacaac taatacagaa
     1321 gactgaaagg tcgtcagcct acgataatct ccccataaaa tgtgacatga atcaggaagt
     1381 tttaacctca cgtgctgcga aatcatcggt gtaaataggg ctatatgccg cgtcttttct
     1441 ggctaatttt atgaaaagat atttattggc ggcacaaaat aaagaacaat tttgaattcc
     1501 ttacattcct ggctattgca caactgaatt taaggctcta ttattacctc aacaaaccac
     1561 cccaatataa gtttgagatt actacaatga gcgaagcact taaaattctg aacaacatcc
     1621 gtactcttcg tgcgcaggca agagaatgta cacttgaaac gctggaagaa atgctggaaa
     1681 aattagaagt tgttgttaac gaacgtcgcg aagaagaaag cgcggctgct gctgaagttg
     1741 aagagcgcac tcgtaagctg cagcaatatc gcgaaatgct gatcgctgac ggtattgacc
     1801 cgaacgaact gctgaatagc cttgctgccg ttaaatctgg caccaaagct aaacgtgctc
     1861 agcgtccggc aaaatatagc tacgttgacg aaaacggcga aactaaaacc tggactggcc
     1921 aaggccgtac tccagctgta atcaaaaaag caatggatga gcaaggtaaa tccctcgacg
     1981 atttcctgat caagcaataa tcttttgtag attgcacttg cttaaaatcc cgccagcggc
     2041 gggatttttt attgtccggt ttaagacaat ttaataagaa taagctataa aaaaacggcg
     2101 tcgattgctc aacgccgttt cgtggataac accgatacgg atgttacttc ttaatgccca
     2161 tctcttcttc aagccaggct ttaaattccg tgccaagggt gttatgacga ataccgtatt
     2221 caacgaaggc ctgcatgtaa cctaatttat taccgcagtc atggctcttc cctttcatat
     2281 gataggcttc caccgtttct ttttcgatca gcatatcaat tgcgtcggtg agctgaattt
     2341 catcaccagc tcccggaggg gtttttgcca gcaacggcca aatatccgcg ctaagtacgt
     2401 aacgacccac aatagcgaga ttagacggcg caacatccgc tttcggtttt tctaccacac
     2461 caaccatcgg tacgctttca cccggcgcta attcaacgcc tttgcaatcc acaacgccat
     2521 atgcggtcac atcagcaacc ggttcaacca tgatctggct atgacccgtt tcatcaaagc
     2581 ggcggatcat ctctgccagg ttatcctgtg acaaatcgga ttcatattca tccagaataa
     2641 catcaggcaa aataacagct accggttcat cacccactac cgggtgagca cacaataccg
     2701 cgtgtcccag gcctttcgcc agaccctgac gaacttgcat aatagtcacg tgcggtggac
     2761 aaatagactg cacttcatca agcagttgac gttttacacg tttttccagc attgcttcca
     2821 gttcaaaact ggtatcaaag tggttttcaa tagagttttt agatgagtgt gtaaccagca
     2881 caatttcagt aatgccagcc gcaatacatt cattcacgac gtattgaatt aatggcttat
     2941 cgacaagtgg cagcatctct ttcgggatgg ctttcgtcgc cggcaacatc ctggttccta
     3001 atcccgcaac ggggataacg gcttttttga ctttcgtatt aatggcagcc atttaaattc
     3061 tcctggactg ttcgtgtttt gaacgtgttc atatttctgt atcgcatccc agtatatcag
     3121 caccccgaag gggctaagtt ctgaaaagga ggcgagatag cataattaag acaattactt
     3181 atacgaatgg cgacgatagt accaccagcc gacattagca ggtaatgcaa atttagcccg
     3241 cgttatcgtt tgctcattct gcagacaaca tcaagcgcag tcgaccaccg gttccccata
     3301 tttggcattg ccaggcatcg catcgctggc tcaattgatt caaataagcg ttacctaaag
     3361 tgcctaacgg aacaccatta ctgatttgca cctggtgttc gccggtattt aacgtcgcat
     3421 tcagacccgc agaaaccaga atgagatttt tcagttcgcg atgataatag ccaactaata
     3481 gcggaaactg ccccggcaga ttggcctgac gaagtaaatg gtttacctgc ttcaataacg
     3541 cgcccaactc tggcaaccgt tgattttggt gtgcaagctg ttcctgtaat aatccgttaa
     3601 acaatgcgcg taataacaag gcagcaagta cgccattatg tccagctcgg gtgacatcaa
     3661 ggcaataaaa tgccagatcg ttttccgaaa gtgcggcaat atcaagcacc aggccgggtt
     3721 tgtccgcggc aaccaattga cggtaattaa cccggcaatg ggaaatcacc tgctgaaccg
     3781 gcggttgtag ttcctgtaat aatttcgccg ctgcggcagg gttatcaacc attgcatccc
     3841 agtcgcgaaa aagcctttcc tcttcctcaa cgcgcgaatt aaacatgctg ggatagagac
     3901 aggcaaaaac catctcgcgc aagcgattca gatctttaac tggtttcagc aaaacatctt
     3961 caacgcccag acgtaacgct ttggcaatat ctgccatatt ttcagtggca gatatcacca
     4021 gaactggggt ctggtcgcct ctgttacgta tatgctccag cagtttaagc ccgttcattc
     4081 gtggcatcgc gatatcacat atcatcaggt ctggagtgaa acctcccagc aactcaaggg
     4141 catccacccc atcagccgcc agtaccgttg tcgctcccaa tgaggaaaac catgaatcca
     4201 gaagcgagcg aaatacctgc tcatcttcaa caatgagaat ctgttttccg accaatggct
     4261 gcgtcatgtt ctctcccctg actggcttta ctcaatagtg gcatgctatt gccacctgcg
     4321 cctgtcagaa ttaacttaag tgtagataaa aattctggtc aaatgttggt gcgtaccaac
     4381 ggcaaaagtt cgtccatttt cctttccact gccagctgtc cggccgcaat ggcagcgtgc
     4441 gcgcgatgga aatcaagcgt agatatttgc gggcaaacag gttgaatcag aatatcgggc
     4501 ggatcacctg ccatgcggtt ccttttaagg cggttctcca acacctggat agacgtggtc
     4561 ataatctctg ttgccgttgg cgctgtcaca gcccgacgtg tcgttatgct gcctaacctt
     4621 tctttcagac gcgcatgcca cggtagagaa tcaccattct cgctattttc ttcactgaca
     4681 ttaaaggaga gcagatcttg ttgcatcaaa tgagcatcgt gctgcaggtc gaccgctatc
     4741 acaatatcag cccccaatgc acgcgtaaga gaaataggaa ttgggttaac tactgcccca
     4801 tcaaccagcc agtagccgtt atgtgcaacg ggtgccatga gtcctggaat actgcatgat
     4861 gcgcgaatag caagatggag atcgccttca gtaaaccata attcacgccc cgtacttaaa
     4921 ttggtggcaa cagccgcaaa gcgacgggaa caattttcga tctctgtttc cggcattatt
     4981 tcgcgatatt gattgaagac acgctcgccg cgcagtaacc caccgcgctg ccaggagagg
     5041 tccatcaggc gtaaaacgtc ccaataactg aaagaggtta cccaatcttc cagcgcagat
     5101 aatcgatcgc atgcataggc cgcgcccacc agcgaaccaa ttgaacatcc tgcaacgata
     5161 tcaatttcaa tacccacttt ttttagcgca ttaataacgc caatatgcga ccaacctctc
     5221 gccgcgccag atcccagcgc cagccctatt tttatctttc tcattattcc cgcaagattc
     5281 ccctggaatg caatcgtagc cacatcgcta ctgctcggtt aacatagtgc tactcaggcg
     5341 gttaatacgc cgttattgtt tccagggaga ttatttgtgt ctcagctttg tccctgtggt
     5401 agtgctgtcg agtatagcct atgttgccac ccttatgtgt ctggtgaaaa ggttgcacct
     5461 gatccagaac atctcatgcg ttcgcgttac tgcgcttttg tgatgcaaga cgctgattat
     5521 ttaataaaga cctggcatcc ctcttgtgga gcagcggcgt tacgtgccga attgatgacc
     5581 ggatttgcgc atactgaatg gttgggattg accgtatttg aacattgttg gcaggacgcc
     5641 gacaatatcg gatttgtcag ttttgttgct cgatttaccg aaggtggcaa aacaggtgca
     5701 ataattgaac gttcgcgatt tttgaaagaa aacggtcaat ggtactatat tgacggtaca
     5761 cgtccgcagt ttggtcgcaa cgatccctgc ccttgtggtt caggtaaaaa atttaaaaag
     5821 tgctgcggcc aataatggtt gacggtacgg tttagcaaac actctcaaca aggtttttcc
     5881 agcaatgcat tcactccaac gtaaagttct gcgtactatt tgtccggacc aaaaaggtct
     5941 gatcgcacgt attaccaata tttgctacaa gcacgagtta aatatcgtac agaacaatga
     6001 atttgttgat caccgtaccg ggcgcttttt tatgcgcacg gaactggaag ggatttttaa
     6061 tgattccacc ctgctggcgg atctcgatag cgcattgcca gaaggctccg tgcgtgagct
     6121 gaatcctgcc ggtcgtcgcc ggatagtgat tctggtcact aaagaagcgc attgccttgg
     6181 cgatttgttg atgaaagcca attacggcgg tctggatgtc gaaatcgcgg cagttattgg
     6241 taaccacgat actttacgtt ctctggttga gcgttttgat attccgtttg agctggtaag
     6301 ccatgaaggg ttaagccgca acgagcacga tcaaaagatg gcggacgcca ttgatgctta
     6361 tcaacctgac tatgtggtgc tggcgaagta tatgcgggta ttaacgccgg aatttgtggc
     6421 acgcttcccg aataaaatca tcaatattca ccattcattc ctgcctgcgt ttatcggcgc
     6481 acgtccttat caccaggcct atgaacgtgg tgtgaagatt attggcgcaa ccgctcacta
     6541 tgtgaatgac aatctggacg aaggcccaat catcatgcag gacgttattc atgtcgatca
     6601 tacctacaca gctgaagata tgatgcgcgc aggtcgtgac gtcgagaaaa acgtcttaag
     6661 tcgtgcgcta tacaaagtac tggcgcaacg cgtctttgtt tacggtaatc ggacgattat
     6721 tctttaatcg ccagcaaaaa taactggtta cctttaatcc gttacggatg aaaattacgc
     6781 aaccagttca tttttctcaa cgtaacactt tacagcggcg cgtcatttga tatgatgcgc
     6841 cccgcttccc gataagggag caggctagta aaaagcatta ccccgtggtg gggttcccga
     6901 gcggccaaag ggagcagact gtaaatctgc cgtcatcgac ttcgaaggtt cgaatccttc
     6961 ccccaccacc atcactttca aaagccccgg aattctcaaa cgaatctgca atcaaatatt
     7021 ctgcccaagc ggggaaggat gagaagcttc gaccaaggtt cgacttgagc gccagcgaga
     7081 gagcgttgcc gcaggcaacg acccgaaggg cgaagcgcgc agcgctgagt aatccttccc
     7141 ccaccaccat cgcttccttg aataatcaaa acctgttact ccgagctaat tttgcccgat
     7201 ggcaccacgt ttaccgggcc tacaatatcc tcccctgtag gtcggataag cgaagcgcat
     7261 ccgacaactc accccgcacc gtgcccataa aaaaaccccg ccgaagcagg gtctatgtga
     7321 actaaaattc gtttagtgac gagcgcgcac cagctggtac ttacgtgtca gatactccac
     7381 cggtacgctc cagatgtgta ccagacgcga gaacgggaac agcaggaata acgtcatccc
     7441 cagcaccagg tgcagacgga agataaacgc cacaccatcg aggtgttgag aagcgccacc
     7501 gtggaaggtc accaccgact gcgcccagcc aaccagtttc atcatctcgc taccgtccat
     7561 atgctgagcg gagaacggaa tggtcagcag gcccagcgcg cactggataa cgagcagcga
     7621 caggatcagg atatccgctc cggtagtggt tgcacgcacg cgtgggctga acatacgacg
     7681 tttcagcaaa agcacgccgc caatcagaca cagcacgccg ctggcaccac cagcaaacat
     7741 cgccattttc tgtttcactt caatcggcag ccaggcttca tacatccagt gcggcgtcaa
     7801 cataccgaag aagtgaccga caaaaatccc cagaataccg atatggaaca ggttcgacgc
     7861 caggttcatc cctttgcgat ccagcatctg gctggacgcc gcgcgccagg tgtactgccc
     7921 gtagtcataa cgcagccagc taccaatcag gaagaccgcc ccggctatgt acgggtagat
     7981 atcaaagaag aacatattca ggaattgcat tagtgctgtc ctccggtggt gatattcaga
     8041 tattgcggcg cgacggcacc ggcaaagcga cgctgatgag cagtgattgc tgaatcaccg
     8101 cagcctttgt cagcaaagaa tttaacctgc tcttcttccc agacagcatc cagcgcctgc
     8161 ggcgtatcat cgcgcgcttc gtcggcaatt ttttccgcca ctttgtcgct gtcgatagcg
     8221 gtattggcca atttcagcag cagatcaaac atcacggcat aacggctttc acgctgttgc
     8281 agacgcgcgc tcagcaatgc cagaatcggc gcgatatctt tcaaaccttc cacggcttcg
     8341 ctttgcggca gctgcgacag gtactccaga tacagcggca gatggtccgg taattcgcgg
     8401 ctgtttaact gcaagccgtg ctgctcgtac tgcgccagca ggtccaccat cgcctggccg
     8461 cggtcacggg attcaccgtg aacatgttca aatagcaaca gtgacgtggc acggccacgg
     8521 tcgaacagtt cgctgtactg gacctgggca tcgagcggat ccatcgccgt taaatcgcgc
     8581 aggaaaatgc ccagcgcatg ggcatcttct ttcgacagat ttttcgacgc agcaatcgcc
     8641 tcaaacatct cttgttgatg ctgccataag gcagcatccg gatattcaag gagacgcgat
     8701 acaatcacga gttcgatcat ggatgcggct ccgttttgct ggtcacatcg atggcatcga
     8761 tacgacggct gttgaacaga ttgaatttgg tatctgagcc gtggcagcca tcaccaaagg
     8821 taaagccgca gccatttttc tccgggaagg cttcccgtgc cagttcacga tgactactcg
     8881 gcaccacaaa gcgatcttcg tagttagcaa tcgccagata acggtacatc tcctgtgcct
     8941 gggcttcggt cagaccgacc tcttccagcg cacgggtatc aactttaccg tcaacagttt
     9001 cagcacgttt gtaatgacgc atcgccagca tacgtttcag cgcacgcagt accggtttgg
     9061 tatcaccggc ggtcagcaga ttcgccagat actgtaccgg aatacgcaag ctttcgacgt
     9121 ctggcagaat tccgttgctg cccagctcgc ccgcgtctgc tgcagactga atcggagaca
     9181 gaggcggcac gtaccagacc atcggcagtg tgcgatattc tggatgcagc ggcagcgcca
     9241 gtttccattc catcgccatt ttataaaccg gcgactgctg tgcagcttca ataacgctca
     9301 gcggaatacc gtctttaatc gcctgctcga tgactttcgg atcgttcgga tcgaggaaca
     9361 cgtccagctg acgctggtag agatctttct cgttctcggt gctggctgca cgttcaatcg
     9421 cgtcggcatc gtacagcagc acgccaagat aacggatacg accgacacag gtttctgagc
     9481 acacggtcgg ctgacccgct tcaatacgcg gatagcagaa gatgcacttc tcagacttac
     9541 cgctcttcca gttgaagtag atttttttgt acgggcatcc ggtgatgcac atacgccagc
     9601 cacggcattt atcctggtcg atcaggacga tgccatcttc ttcacgcttg taaatcgcac
     9661 cgctcgggca ggtcgccaca catgccgggt tcaggcagtg ttcgcacagg cgcggcaaat
     9721 acatcataaa ggtgttttcg aactggctat acatcgcctt ctggatgttg tcgaagttct
     9781 tgtctttcgc cagtttatca aactcaccgc ccaggtcatc ttcccagttc ggcccttttt
     9841 cgattttcgc catccgttcc ccggtaatca gcgaacgcgg acgggcaatc ggctgcgatt
     9901 tgctgccttc cggcgcggta tgcaggttct gataatcaaa atcgaacggc tcgtaataat
     9961 cgtcgatccc cggcagatgc gggttagcaa agattttacc cagcagcatg gctcggttac
    10021 ccatgcgcgg ttgcagtttg ccattgattt tacggatcca gccgcctttg tatttttcct
    10081 ggttttccca gtcagtcggg aagccctggc ccggcttggt ttccacgttg ttgaaccacg
    10141 cgtattccac gccttcacgg ctggtccaga cgtttttaca ggtaactgaa caggtgtggc
    10201 agccgatgca cttatcgaga ttcagcacca tgccgacttg tgaacgaatt ttcattttac
    10261 gctctcctgt acctggtcat tgccttcgcc atctaaccag tcaatgttct tcatcttacg
    10321 cactacaaca aactcatcgc ggttagaacc tacggtgccg tagtagttaa agccgtatgc
    10381 cagatgggca tagccgccga tcatatgcgt cggtttcggc gtgatacggg tgaccgagtt
    10441 atggatacca ccacgctgtt gggtaatttc cgaaccaggc aggttaacga tacgttcctg
    10501 cgcgtggtac atcatggtca tccctgccgg aacacgctgg ctgacaaccg cacgggcagt
    10561 cagtgcaccg ttgctgttga agacttcaat ccagtcgtta tcggcgatac ccagatcttt
    10621 ggcatcggct tcactcaacc agaccaccgg accaccgcga cctaaagtca gcatcagcag
    10681 gttgtcgctg taggtggagt ggatacccca cttctggtgc ggcgtcagga agttgagcgc
    10741 tttttccggg ttgccgttgg atttctggcc tatcacttct ttcaccgaac gggtgtcgat
    10801 cggcggacga taaaccagca ggctttcacc gaaatcacgc atccactggt gatcctgata
    10861 cagttgctga cgaccagaga gcgtacgcca tgggatcagc tcgtgaacgt tggtgtaacc
    10921 ggcgttgtaa gaaacgtgtt catcttccag accagaccag gtcgggctgg agataatttt
    10981 gcgcggctgt gcctgaatat cgcggaagcg gatcttctcg tcttctttat tcagcgccag
    11041 atgcgtatgg tcacgaccgg taaattcgct cagggcagcc caggctttca cggctacctg
    11101 accgttggtt tccggtgcca gcgtcaggat catctctgcc gcatcaattg cggtgttcag
    11161 catcggctgg ccttttgccg gaccttccgc tttggtgtag ttgagcttac gcagcagatc
    11221 catctcgctc tgggtgttcc aggcaatccc tttaccgccg ttaccgattt tctccatcag
    11281 cgggccgata gaggtaaagc gttcgtaagt tgccggataa tcgcgctcta cgaccataat
    11341 gtgcggtgca gttttgcctg ggatcaggtc gcattcgcct tttttccagt ctttcacatc
    11401 cagcggctgc gccagttcag cggcagagtc gtgctggata ggcagcgtga cgatgtcggt
    11461 ttctttaccc agatagccga cgcacacttc ggagaatttc ttcgcgatgg ctttgtagat
    11521 ttcccagtcg cttttcgctt cccaggccgg atcgaccgcc gcagacagcg ggtgaataaa
    11581 cggatgcata tccgaagtat tcatgtcgtc tttttcgtac caggtcgccg tcggcaaaat
    11641 gatgtcggaa tagagacagg tgctcgacag acggaagtcc agcgtaacca ccagatccag
    11701 cttgccttcc agaccattgt cctgccagtc cacttcttcc ggcttcacgc cgccctgttg
    11761 ccccagatct ttaccctgga taccgtgctc cgtccccagc aggtacttga gcataaactc
    11821 atgaccttta ccggaagaac cgagcaggtt agaacgccag atgaacaggt tgcgcgggtg
    11881 gtttttaccg ttttctggtt gttctgccgc aaaacggatg gaaccctctt tcagggattt
    11941 caccgtatag tccaccggat tcatcccggc tttttccgct tcgccagcga tagtcagcgg
    12001 gttagtgcct aactgcggtg cagacggcag ccagcccatg cgttccgcac ggacgttaaa
    12061 gtcgatcaag tgtccggtat agcgggattt gtccgccatc ggtgacagca actcttccgc
    12121 cgtgacggtt tcataacgcc actggctgga gtggttatag aaataagaag tgctgttcat
    12181 gtgacgcgcc ggacgctgcc agtcaagggc aaacgccagc ggctgccagc cggtttgcgg
    12241 acgcagtttt tcctgaccta catagtgcgc ccagccgccc ccgctctgac cgacacagcc
    12301 gcagaaaatc agcatgttga tcagaccacg atagttcata tcgaggtgat accagtggtt
    12361 cagccccgca ccgacgataa tcatcgaacg accgtgcgtt ttatcagcgt tatcggcaaa
    12421 ttcacgggcg atgcgaataa tctggctgcg agaaacgccg gtaatctgct cggcccaggc
    12481 cggggtataa gctttcacat cgtcatagct ggttgcacag ttaacgtcgt tcaggccacg
    12541 ttccagaccg tagtttgcca gcgtcagatc ataaacggtg gtcaccaggg cggtgctgcc
    12601 atcagccagt tgcaggcgtt tcaccggcag tttgtgcagc agcacgtttt ccagttccac
    12661 tttgttgaag tgttccgtgc cgtcgccacc aaagtacggg aagcctacct cagcgatctc
    12721 atcctggcta cccagcaggc tcagttgcag ctcggtttct tcgccagttt tgccgtcgcg
    12781 ctgctcaaga ttccatttgc ccttctcgcc ccagcggaag ccaatagaac cgttcggcgc
    12841 aaccatttcg ccattggtat taaaggcgac agttttccat tccggattgt tttcctggcc
    12901 cagcgcatca accagatcag cagcgcgcag catacgacct gcagcgtagt aaccgtcgcg
    12961 ttcttccagc atcaccagca tcggcatgtc ggtgtagcga cgcacatagt cggtgaaata
    13021 ctggcttggg ttgtcgaggt ggaattcacg cagcattacg tggcccatcg ccagcgccat
    13081 tgccgcatcg gtgccctgtt tcggtgccag ccacagatcg cacagtttgg cgatttcagc
    13141 gtagtctggt gtgacggcaa cagttttggt gcctttgtaa cgcacttcag taaagaagtg
    13201 agcatccggg gtacgcgtct gcggcacgtt tgaaccccag gcgatgatgt agctggagtt
    13261 ataccagtca gcagattccg gtacgtcagt ttgctcgccc caggtttgcg gagacgcagg
    13321 aggcaggtcg cagtaccagt cgtagaagct taagcaagta ccgccaatca gcgagagata
    13381 gcgtgcaccc gatgcgtaag aaaccatcga cattgccgga attggcgaga aaccagcaac
    13441 acggtccggg ccgtagtttt tgatggtgta aacgttagat gcggcgatca gttcgttcac
    13501 ctcctgccaa gaagaacgaa caaatccacc gcgtccacgc gcctgcttaa agcttttcgc
    13561 tttatcggcg tcttcaatga tagaagccca tgcctcaacc ggatcgctat gcagcgcctt
    13621 cgcttcacgc cacattttca tcaggcgttt gcgcatcatc gggtatttca ggcggttggc
    13681 actgtaaaga taccaggagt agctggcacc gcgcgggcag ccgcgaggtt catggtttgg
    13741 cagatccgga cgggtacgcg gatagtcagt ctgctgggtt tcccaggtga ccagaccgtt
    13801 tttgacgtag attttccagc tgcaggagcc ggtgcagttt accccgtggg tagagcggac
    13861 gattttgtca tgctgccaac gctggcgata tccatcctcc cagtcacggt tggtattgag
    13921 aagctggcca tgcccatcgg caaaggtttc acccttctgt ttgaagtagc gaaaccggtc
    13981 caggaattta ctcatcggtt ttctcctgtg ggagcctgtc ggccctctga taaatcgaca
    14041 ttgctaatgt ttaagggcaa ggtaacgctc tgaaacagcg tgggaattga taacgatcaa
    14101 gagtgatggg gatgaaaaat aaagtaaatt cctaagatac ccctttagga taattaacag
    14161 ttacagcata acatgctgta aattaagacg ttttatacct atagttattt aaactattaa
    14221 gatttttatg caaatgggta ttaaggagta ttccccatgg gtaacatatt aatggctctt
    14281 aatgaaatcc ggcgaaataa gccatataac actaatatgt tgtaactaaa tgacgcataa
    14341 aaaaagcgcg gcatcatgcc gcgcaaagga taatcaaata ttaagattta ttttttagaa
    14401 tgccgaccat ataccgccca agtaatcact acgcaggcga tatagaaaat caaaaatact
    14461 ttcattgcac ctactggcga acccgttaat gccagcgagc taccaaacgc tttcgggata
    14521 aagaagccac caatcgcgcc aatcgcagag atgaaaccca gcgccgctgc cgtgccggtt
    14581 gccgcttcac gcatcgcacg ttcgtcagaa cccccttctg ctttcacgcg atccattgtc
    14641 agtttacgga agatcactga aatcatctgg aaagtggaac cactacccag cccagctgtc
    14701 aggaacagcg ccaggaagac cgcgaagaac gccatgaagc ttccgccctg cccgtcagtc
    14761 ggtaaggtca ggaacagcag gccgctgaaa atcgccatca gaataaagtt caccagcgtg
    14821 acacgagttc cgcccagacg gtcagataat gcaccacctg cagaacgcgc cagcgcacca
    14881 ataaacggcc cgaagaaagc gtattgcaga atctgaacat ccgggaactg cgtttttgac
    14941 agcatcgcaa agcccgcgga gaagccgatg aaggagccga aggttgccag atacagcagg
    15001 ctcataatcc acagatgacc ccgtttgagt accggcaact gctccttgat ggaggctttc
    15061 gaggtagcaa gatcgttcat gccaaaccac gccgcaatgg tgaagatggc aaggaacggc
    15121 acccatatcc aggacgcatt cgccagatac agctcagtcc catccggctg tttgacgccc
    15181 tggctaccaa atactgcgaa aatcgacagt gataccacca gcggagcaac caactgcatg
    15241 acgctgacgc ccatgtttcc cagaccacca ttcagaccca gcgcgccacc ctgcttctgt
    15301 ttcggaaaga agaagctgat gtttgccata ctggatgcga agttcgcgcc agcaaagccg
    15361 catagcagag agatgatgat gaagacgcta taaggcgtgg aggtatcctg cacggcaaaa
    15421 cccagccaga cgcaaggaat aatcagaata ccggtgctga acgccgtcca gcgacgacca
    15481 ccgaagatag gaaccataaa ggagtatgga acacgtaata acgcgccaga aaccgaaggc
    15541 agcgcagtca acataaatag ctgatcggtc gtaaaattaa aaccgacttt cggtaagttc
    15601 accgcaacag cgctgaacaa catccatacg caaaacgcca gcagcagaca gggaacggaa
    15661 atccacaggt tgcggctggc aatacgttga ccgcgttgtt gccagaacgc aggatcttcc
    15721 ggtcgccaat ctgttatgac agctccagta gccctttcgg aggcagatga gtgactcata
    15781 gacacctctg atactcgttt cgatgtctgc caccttagtg tctggagcta aaggcaattt
    15841 gatgtaaatc aaacgataag ataactttat ctatcattga tatttatcat tacccatagt
    15901 gagtacagtg atttcataaa aattatgaga tttttcacgg tgctgtagaa tccctaccct
    15961 taccgatgta aagcgactaa ccacacggca aataaggagt aactctttcc ggctatgggt
    16021 atacttcagc caatagccga gaatactgcc attccagaat atatcgtcac attcattaag
    16081 gttattgctc atttaaagcc tgaaggaagg ggtttacatg cttaaacgtt gtctctctcc
    16141 gctcaccctg gttaatcagg ttgcgcttat tgtgttgctt tctactgcta ttggactggc
    16201 agggatggcg gtttctggct ggctggtgca aggcgttcag ggcagcgccc atgcgatcaa
    16261 caaagcggga tcgctgcgca tgcaaagtta ccgtctgttg gcggcagtgc cattaagcga
    16321 gaaagacaag cccttaatta aagagatgga acaaacggca tttagcgccg agttgactcg
    16381 agcagcagaa cgagacggac aactggcgca attacagggc ttacaagatt actggcgtaa
    16441 tgaactgatc cctgcgctga tgcgtgcaca aaaccgagaa acggtgtcag cggatgtcag
    16501 ccagtttgtt gccgggcttg atcagctggt atctggtttt gaccgcacca cggaaatgcg
    16561 tattgagaca gtggtactgg tccatcgggt aatggcggta tttatggcac ttttactggt
    16621 gttcactatt atctggttgc gggcgcgact gctacaaccg tggcggcaac tgctggcaat
    16681 ggcgagtgcc gtcagccatc gcgattttac ccaacgcgca aacatcagcg ggcgcaacga
    16741 aatggcgatg cttggaactg cgttgaacaa tatgtctgca gaactggccg aaagttatgc
    16801 cgtacttgag cagcgggttc aggagaaaac cgccgggctg gagcataaaa atcagatcct
    16861 ctctttttta tggcaggcta accgccgttt gcattcccgc gccccgctgt gtgaacgcct
    16921 gtcaccggta ctcaacggct tacagaattt aaccctgcta cgtgatatcg aactgcgggt
    16981 gtatgacact gatgatgaag agaatcatca ggagtttacc tgccagccag atatgacttg
    17041 tgatgataaa ggctgccagc tctgcccgcg cggcgtatta cccgttggtg atcgcggcac
    17101 gaccctgaag tggcggctgg ctgactctca tacgcagtac ggtattttgc tggcgaccct
    17161 gccgcagggg cgtcatctta gccatgacca acaacaactg gtggataccc tggttgaaca
    17221 acttaccgcc acgctggcgc tggatcgcca tcaggaacgt cagcaacagt tgatcgtgat
    17281 ggaagagcgt gccaccattg cgcgcgaact gcatgattct attgcccaat ctctctcttg
    17341 catgaagatg caggtgagtt gtttacagat gcagggcgat gcgctgccag aaagcagccg
    17401 cgaactgtta agtcagatcc gtaacgaact gaatgcatcc tgggcgcagt tgcgtgaatt
    17461 gctcaccaca ttccgcttgc agctcaccga gcctggatta cgtccggcgc tggaagcgag
    17521 ttgcgaagag tacagcgcca aatttggctt cccggtgaag ctggattatc aattgccgcc
    17581 tcgtctggtg ccttcacatc aggcaatcca cttgttgcaa attgcccgtg aggcattaag
    17641 taacgccctc aaacattcgc aagcgagtga ggtcgtggtg acggtggcgc aaaacgataa
    17701 tcaggtcaaa ctgaccgtcc aggataacgg ctgtggcgtg cctgaaaatg ccatccgcag
    17761 caatcactac ggcatgataa taatgcgcga ccgtgcgcaa agtttacgag gcgattgccg
    17821 cgtccgccgt cgtgaatcag gtggcaccga agtggtggtc acctttattc ccgaaaaaac
    17881 tttcacagac gtccaaggag atacccatga gtaatcagga accggctact atcctgctga
    17941 ttgacgatca cccgatgctg cgaactggcg taaaacagct tatcagtatg gcaccagata
    18001 tcaccgtggt tggcgaagcg agtaatggcg aacagggtat tgaactggcg gaatctcttg
    18061 atcccgatct gatcctgtta gatctcaata tgcccggcat gaacggtctg gaaacgctgg
    18121 ataaactgcg cgaaaagtcc ctctcagggc gcattgtggt attcagcgtc tctaaccatg
    18181 aagaagatgt ggtcaccgca ctgaaacgcg gcgcggatgg ctatctgtta aaagatatgg
    18241 aaccggaaga tctgctgaaa gcattgcatc aggcagctgc tggcgaaatg gtattaagcg
    18301 aagcattaac gcctgttctg gccgccagct tgcgcgctaa ccgtgccact actgagcgcg
    18361 atgttaacca gttaacccca cgcgagcgcg atattctcaa gctgattgcc cagggtttgc
    18421 cgaacaagat gattgcccgc cgcctggata tcaccgaaag cacagtaaaa gtgcacgtca
    18481 agcacatgct gaagaaaatg aagctcaagt ctcgcgtgga agcagcggta tgggtgcatc
    18541 aggagcgcat tttctgatta cggttcccag cgcagttcgt cgtttgacaa tgcatcgaac
    18601 ggttcgacaa gcgttagcgt gatctcattg gaggagacac gctgcccctg gttatcttcc
    18661 accaccacag acaatcgcca gtgattgctt gccccttccc cgttctgcca gtcaggcatg
    18721 atcagcgtcc agccctccgc gctgttggct tgtgcgcccg gcgtcaaact taatatctgc
    18781 gtatcgccct gccaaatcag ttgccgaata ccgtaacgac tgcggatttg taatttcagc
    18841 ggcactgttt cgccaggttt tagatcccac ggcggtgtcg ccagaaacac cgttaacgtt
    18901 tttcgctgtc ggtactcaag agtcggtaga ttatttcgct gcggattgtc atagcgacta
    18961 ccacgtaacg actgactttc ggcaacctcg cccgcagaaa gttgtttttt gagcggtaca
    19021 ccaaagcggt aattaagatt cagcccgagg ttattttgat tttcgccact ttccccctgt
    19081 ttatgctggg ccgtcacagt gactaatggc acaggggtgt aatttaatcc cagactcaac
    19141 gcgacgggat tgtgataacc cgtaccagag ttaaacaaat caacacgatc accaaaatac
    19201 tgttctaggc tgacactggt attgaggtgt tgatagaacg gcatgcgcat ccgagctgtc
    19261 aggtcgtacc cgcgcgccat ccgttgttcc tgcgtggctg tctgttcatg ccatgcagca
    19321 aacggctgat aaaagtttgc cgataatcgc aaatattcgc cccacgcttc ggcaccaaag
    19381 cccgctcgct gaagattttc gtcctgcaag ttgtcataaa aagtgttata acccaccagc
    19441 cagttgccgc gcgcccagcg ttgcccaacg cccacattgc tcaccaaccc attatcctgc
    19501 tgagtaagac caagctggct ccaggtgaga taacgatcat tatcttgtaa cggcacaaac
    19561 cagcttccac gactgccggt gaaatgtcct tcgttatcca ctttgacgtc aacactggca
    19621 tttccccacg gtgatagcca ggactctacg tgctgattaa cctgttgact aagcgcgtcg
    19681 cggacttttc ccaatgcgaa agcttttgcc tgctcgccag tatccagccc gttatcattc
    19741 atactggttt cgccgaaatc tttcacaatt tcagcaaagt gtttttcccc atcatgattt
    19801 tcgggagcca tgcctaaatc cggcagacca tcattgttat tatcaaaggg atttgccgct
    19861 ttttgctcga aggtagattg tgcgttagct gtaccgcctg ctacaagcaa gagtaaataa
    19921 aacgggataa tgcgaggaac gaaacggctc aaaacgtcgg taacttccgg taataatgaa
    19981 aaaccatagc cttaaagaat aacgattact ggcaactttt gtcgtatttt ctcgcgcttt
    20041 caggaaaatc cttaaaaatg tgaccgattg gtcagacgct gcagaagcag taagatagaa
    20101 gatgagtgtc taattccctg aatacaggag taaataatgc aaaaaatagt gatcgttgcc
    20161 aatggcgcac cttacgggag cgaatccttg tttaacagct tgcggctggc cattgcgtta
    20221 cgagagcagg agagcaatct ggatctgcgt ctgttcctga tgtctgatgc ggtcacagcc
    20281 gggttgcgcg ggcaaaaacc aggggaaggc tacaacattc agcaaatgct ggagatcctt
    20341 accgctcaga atgtaccggt gaaattgtgc aaaacctgta ccgacgggcg cgggattagc
    20401 acacttcctc tgattgatgg ggtggaaatc ggtactcttg tggaactggc gcaatggacg
    20461 ctgtcagccg ataaagtgct cacattttaa tgtttaaggg ggcgtctgac gccccactat
    20521 gagggaagtt tactcaggcg aatcccggcc ttaacacacc atccggataa ttctccgcca
    20581 gtagtttttt taccgatacc agcaaatcat tcagcccatc gtcctgcatt cccagtttga
    20641 taagctcctg ttccagtgaa aacaggtatt gtgcattggt tcccagcgga ccgctcgccg
    20701 ccgcaatcaa cggcgcgatg acctgagcgc gagtgtcaga ttcatattct ggatgtcgcg
    20761 ggtccataat aaacacaatg gcgtttactg tgcgtccatc atcaagatca agctgacacc
    20821 aggttggcag ataacagccg gtaatcatct cgcgcttcca caacagggtt agttcctgct
    20881 ccagcgtctc ttctggtagt cgataggcga cgcctgtggt gcgtccgccc tctttcagtg
    20941 caagcatccg tcccggctgg tgcgcagtcc cgcgcccggc ggtcaggcgc aggcaaaacg
    21001 cgcgatgcca tccaaccagt gtaccggtgc atgattcggt aaactccagt gccggattcc
    21061 acatcaacga tccataaccg aagatccaca ccggtccctc atcaggtcga caagccagcg
    21121 tcgccgccag cgaagccgcc cgttgttctg ctgaccataa gagtgattct tcaatggcac
    21181 caaatgccgt tttacaatcg gcattcatca agaaatcacg cgttatcacc ttacacctcc
    21241 gccactgcat acttccctgc gacaactttt tggtgtcttc cggacatttt taccgctcat
    21301 tttgaaacca ctaacggaac aatatgcaat ccacggacca cttgcaaggc aacaccgcac
    21361 gaatagcttt aactaagcta ccggttttac gattttttat gccatttatc atcatcccct
    21421 ttggcatatt catgcttcac agcagcccag gccactttat gcgcggtttc ttcgcgactg
    21481 gcgtcatcac gccgatcttc tttatcttta tattgatccc atgcgctgtt gaacgcttct
    21541 ttatagatat cctgggcatg agacggtaga acgtgcttta cgctttccgg cagatcgctt
    21601 ttcgttttat acggcatcgt tgactccttt tacaaagttg aacattaagt gtggtcaaca
    21661 atatctactc ttgccaaaac ataagaattt tcatgtcaga ctgttcttaa gaaacgctaa
    21721 aacaaacatt gtctaaacgt aatacttatt tttatgcccc aatgagtaaa aaatcgacag
    21781 cggggtaaaa attcgtaaaa tgttaccgat atccagacac tttctgttat ttttcctacc
    21841 tattttttac attttcgata aattcatctg caaagggata accataatgt caaatgctca
    21901 agaggcggta aaaacccgcc acaaggagac ttcgcttatt ttcccggttc tggtgctggt
    21961 agtgctgttc ctgtggggaa gtagccagac actaccagtg gtcattgcca tcaatcttct
    22021 tgcgcttatt ggtattttaa gtagcgcctt tagtgttgtc cgtcatgcgg acgtattagc
    22081 ccatcgcctg ggagaacctt acggttcgct tattcttagc ctttcagtgg ttattcttga
    22141 agtcagtttg atttcagctt taatggcaac cggcgacgcc gcgccaacgc taatgcgtga
    22201 tacgctctat tcaatcatta tgattgttac cggtgggctg gttggctttt cattattgtt
    22261 gggcggtcgt aagtttgcca cccaatatat gaatctgttt ggtatcaagc agtatttaat
    22321 tgcgctgttc cccctggcga taatcgtact ggtatttcca atggctctgc ctgcggcgaa
    22381 tttttcaacc ggtcaggcgt tactggtagc attaatttct gcggcaatgt atggcgtatt
    22441 tttgctgatc caggccaaaa cgcatcaaag tttgtttgtc tacgagcacg aagatgacag
    22501 tgatgacgac gacccgcacc acggtaaaac gtctgcccat agcagcctgt ggcatgctat
    22561 ctggttgatt atccatctga ttgccgttat tgcagtcacc aaaatgaacg ccagctcgct
    22621 ggagacattg ctcgacagca tgaatgcccc tgttgccttt actggcttcc tggtggcact
    22681 gttgattctg tcgccggaag gtttaggtgc gttaaaagca gtgttgaaca accaggttca
    22741 gcgcgcgatg aatctgttct ttggttcagt gttagcaaca atttcgctta ccgtacctgt
    22801 cgtcacgcta attgccttta tgacgggtaa cgaattgcag tttgcacttg gtgcgccaga
    22861 aatggtggtg atggtggcct ctttagtgct gtgccatatc tccttctcca ccggacgtac
    22921 taacgtgctc aatggcgcag cgcatctggc actgtttgcc gcctatttga tgacgatatt
    22981 tgcctgacag ttatatctcc tccgatgacg gaaatcggga gagataagat aaattcctgc
    23041 ttattgtatt tcttcatact acctggacag ctcgttaatc agaattgcga gcctgtacca
    23101 caacctgcac gaaaccactg caacagctgt tgaaaagccg gaatgtactc tttattgcaa
    23161 acatttcagg gatgaagtga agatgaattg agacgtagta tccacaccaa gccggaatta
    23221 tccggtgagg cgcaatgttg cgggggcttt atccctggtg gcattggttg ctggaaagag
    23281 aaaacccccg cacgttgaga ggtaaaacct gacaacaaaa cgggggctaa tcttgaaacc
    23341 agacacttca agaatagccg caaaatgttg tcattacaac aaggcggcta tatgacgctc
    23401 gcgcagtttg ccatgacttt ctggcacgac ctggcagcac cgatcctggc ggggattatt
    23461 gccgcagcga ttgtcagctg gtggcgtaac cggaagtaag gtgaaaacgg gcgtcaggct
    23521 gccgtgatgg caatatgcgc ccgaaaccgg ccgcaggggt aacagcctgc ggcctttttt
    23581 gtatctggca cccatgaaaa aagtcttaac gcagaacgct aagactttat ttttcaagca
    23641 aaaaagatta cttgctggta tccagttctt cgaaaccttt caccagatca tcaatcgctt
    23701 tcatctgctt gaggaacggt tccagtttag ccagcggcag cgcggatgga ccatcacatt
    23761 tcgcatgttc cggatccgga tgcgcttcaa taaacagccc cgccagacct accgccatac
    23821 cggctcgtgc cagctcagcc acctgagcac gacgaccacc ggaagctgcg ccaaacggat
    23881 cgcggcattg cagtgcgtgg gtcacgtcga aaatcaccgg cgagttacca gacactttct
    23941 tcataatgct gaagcccagc atatcgacaa ccaggttgtc atagccgaag ttagcaccac
    24001 gatcgcaaag aatcactttt tcgttgccgc cttctttgaa tttatcaacg atattaccca
    24061 tctgtcccgg gctgacaaac tgtggtttct tgacgttaat taccgcaccg gttttcgcca
    24121 tggcttcaac caggtcagtc tggcgagcaa gaaacgccgg caactgaatc acatccacga
    24181 catcagcaac gggctgtgcc tgacttggtt cgtgaacgtc ggtgataatt ttcacgccaa
    24241 aagtctgctt caactcctgg aagattttca tcccttcttc caggcccggt ccacgataag
    24301 agtggatgga ggagcggttg gctttgtcaa aagaggcttt gaacacgtaa gggataccca
    24361 gtttctgggt cacagttacg tagtgctcgc aaatgcgcat cgccagatcg cgagattcca
    24421 gcacgttcat accgccaaac agtacgaacg gcaggtcatt tgctacgttg atgtcgccaa
    24481 tgctaaccac tttttgtttc ataggatcgc cttaatcttg agtaaaatgt cgattaatta
    24541 atgcagcaca atatgtttat gcgcgatgtt atttatttgc gcacggatca tttcgctgat
    24601 cgggtcttcc ggacaccgtt caacgaaata acttaaatcg ttcaacgcaa cgtgttcgca
    24661 atccagttgc gcataaatca acccacgatc gcgaatttca tagggatctt cagggttgaa
    24721 ttgtaataaa gcttcgctgg tgcgtaacgc cagctccatc tgattttctt ccatcaacga
    24781 ggctttgagt gtatccagca atttgcggat tacctcaatg ttatcagctt catcaaggtc
    24841 ttcataaaac agttccgccg acgggctgat atttcccttt aaccatccgt ccagcatatg
    24901 ttcgcttaac gattcaccgt taaaaggatt aatcagccaa atttcgccat ccggacattc
    24961 aatgcgcaat atcagctgcg tagggaaaat caccggcagc agcggcaaat cgagacgatt
    25021 cgcgacccat aataaaaccg cacctaatga taccgcactg ccctgtcgat tctttaacac
    25081 ctggtccagc cataatgcat cggaaagacg ataaacgccg cgtgaggctt taaatcccca
    25141 gtcgccgtaa aacagcgcga tcagtttttc cagctgctct tctaaaggca gaagctggct
    25201 gatttcttcc ttcgccagac taacgagacg ctccagttcg tcgtaaacat cttgcgaggg
    25261 aaaatcgcgg cggattgctt cgcaagccag gatcatgcct tcgcacaatg gcgctttatt
    25321 aaattcgaaa tcagctaacg atctcatgac ttaccccagt aacggtactt ttgtggtggc
    25381 gagtttaatg atgatgtaca gcaccaccaa cgccagcggg aaggcaataa tgcgcgcctg
    25441 ctgactgtgc atacgacgat aatcgagtgc aataaaaccc aaaacgatat aaatgataac
    25501 tccaaacagc ttttcagtca gccactgtgc ctgcccggaa aatggcagga tgtgcgcttt
    25561 agccatcaac gcaatgccgc ttaacaataa caccgtatcg acaaccggcg gcacaatgcg
    25621 cgtccagcga gcaaatgcct gtggatgctt ctgataacgt agccagaaac gtaaggttaa
    25681 tagcccaaca gaaagcgcga tactaataag atgaacacta agcagtgtag aaaaacttgt
    25741 cattgataat agcggccgag cgttacgcgc tcgttatcac catagtcacg gcaggtttcg
    25801 acgtcatgat accccgcgag gataaatgct tgtcgcaccg cttcgccctg ctgccagcca
    25861 tgttccagaa gcagaaagcc gccggatacc agcgcgttac gcgactgttc gatgatatgc
    25921 acgatgtctg ccattccact gtctgccgca accagcgcag tgagcggctc aaagcggaca
    25981 tcgccttgtt gaagatgtgg gtcctgctcg tcaatatacg gcggattgct gacaatcatc
    26041 gcaaactgct gcccggctag cgcgctaaac cagtcgcttt gcagaatgtg gatatttttg
    26101 atcgccagat gctgggcatt acgttgtgcc agggagacag catcaggcat acgatctaca
    26161 gcgataattt cgcagtccgg gcgctcgcta gccagcgcaa gcgcaatcgc cccggtaccc
    26221 gtcccgagat cgagaatacg gcaaggttgt tcaggcaacc gcgccagtgc ctgctccacc
    26281 agacactccg tatccgggcg cggaattaag gtcgctggcg aaacaaataa cggcaacgac
    26341 cagaattctc gcaccccggt taaatgagca atgggttcac catcgcgacg acgtgtcagt
    26401 agcgcatcaa gttgctgaca ttgttcgtca gtcagctgcg tttcaccaaa ggcgagaata
    26461 aaagtacgcc ctctgccggt aacatgctcc agcaggattt cagcatcacg ccgcgggctt
    26521 tcgctcgcct gaagttggct tattgcttca cgtaaccagt gttgatattc cattattcct
    26581 gctcggacaa cgccgccagt tggtcggcct gatgttcctg gataatcggt tcaatcagca
    26641 tatccagctt accttccatc acttcatcca ggcggtagag cgtcaggttg atgcggtgat
    26701 cggtaacgcg cccctgcggg aaattgtaag tacggttacg gtcgctgcga tcgccactcc
    26761 ccagcaggtt acgacgggta gacgcttcgg cctgttggcg ttttgccatt tcagcagcgt
    26821 ggatgcgagc accgagcact gaaagcgcct tcgctttgtt tttatgttgt gaacgttcgt
    26881 cctgacattc aacaacaatc ccggtcggca agtgagtaat acgaattgcc gaatcggtgg
    26941 tgttaacgtg ctgaccaccc gcccctgacg agcggaaagt atcaatgcgt aaatctgctg
    27001 ggttgatgtc cggcagttct gcgtcaggca gttctggcat taccgcaacg gtacaagcag
    27061 aagtatgaat acgaccctgc gattccgtag caggaacacg ttgcacgcga tgaccgccgg
    27121 attcaaattt cagacgacca tacacaccat caccgctaat tttggcgatg atctctttat
    27181 aaccaccatg ttcaccctcg ctggcgctca tgatttctac ccgccagcgg cgggcttcgg
    27241 cgtaacggct gtacatacgg aacagatcgc ctgcaaacag cgctgcttcg tcgccgccgg
    27301 ttccggcacg gacttcgagg aaggcgttac gttcgtcatc aggatctttt ggtagtaata
    27361 gaacctgtaa ttgctgttcc agttgctcgc ttttttcttt agcttcgcgc agttcatcct
    27421 gcgccatctc acgcatttca ggatcgtcga gcatcatctg tgcggtttcg atatcttcct
    27481 gaacctgttg ccagtcggta aaacagcgcg aaacatcact taactgcgca tattcgcgtg
    27541 ataatgcgcg aaaacgttcc tggtcggcga tagtttgcgc gtcacccagc aacgcctgaa
    27601 cttcttcatg gcgttcatgc agggcttcca gtttggcaac gatagaaggc ttcataggcg
    27661 taaatgcacc ctgtaaaaaa agaaaatgat gtactgctac tccagcccga ggctgtcgcg
    27721 cagaatattc aggcgttcgt tatccccgtc acgggcggcc tgttgaagtg atttcgttgg
    27781 cgcatggatc aagcggttag tcagtttcca tgccagatcc tgcataatgg cttgcgcgtc
    27841 gccgccctgc tcaagggccg ctaacgcttt ggcggttaac tcatcgcgaa cgtgctctgc
    27901 ctggctgcga tactcgcgaa tggtttcgct ggcgctttgt gctcgcagcc acgccataaa
    27961 ttcgctggtt tcctgagcga caatagtttc cgcctcaacc gctgcggctt tacgctgcgc
    28021 caggttgtgc gaaatgatgc tttgcagatc gtccacacta taaagataag cattcgccag
    28081 tttgccaact tccggctcaa catcgcgcgg aacggcaata tccaccaaca gcattggttg
    28141 gttacgacga ctttttaatg cgcgctccac catgcctttc ccgataatcg gtaacgggct
    28201 ggcggtggaa ctgatgatga tatcggcttc gcgcagacgt tcgtcgatct cactcagggc
    28261 aatcacttcc gcgccaactt catctgccag aatttgggca cgttcgcgag tgcggttggc
    28321 gataatcatc ttctgtactt tatgttcgcg cagatgacgc gctaccagct cgatggtttc
    28381 gcccgcgcct accagcaaca ctgtgaccgt agagagcgat tcaaagatct gccgcgccag
    28441 cgtacaagcc gcaaaagcga cagacacagc actggcaccg atatctgttt cagtgcgaac
    28501 gcgtttcgct acagagaaag atttctggaa catgcgttcc agttcgctgg ccttcatatg
    28561 acctttttgc gaatcgacaa acgctttttt aacctgaccg aggatctgcg gctccccaag
    28621 aaccagtgaa tccaggccgc tggcaacacg cattaaatgg ctaaccgcgt cgttatcctg
    28681 atgccagtag aggcttttac gcagatcttc ttcattaaga ttgtgataat cgcaaagcca
    28741 gcggattaac gcctcttgca ggttatcctg ctcttcaacg ctaagataaa gttccgtgcg
    28801 gttgcacgtc gacagcacca cgccgccctg caccatcggc tgcgcaagca ggctgtcaag
    28861 cgcctgatcg agcttatccg gcgaaaacga tacacgttct cgcagcgata caggtgccgt
    28921 tttatggttg ataccgagtg ctaaaagggt catgtctgcg ggaaataata ccaacgttga
    28981 tagggttagt ctgcttgcat catacaggat gcgtaagatc aataaaagag agcgccccct
    29041 tttggagtaa ttgccgaaag ccgttagatt ctggcaatta agacaacttg aacatagacg
    29101 atagcggacg gtaacgctag cattaagggt tataactgca acgtatctca aggacttgtc
    29161 atcactatgc ccctgcccga ttttcgtctt atccgcctgc taccgctggc tgctcttgtg
    29221 ctcactgcct gttccgttac cacgcccaaa ggtcctggca aaagcccgga ttcgccacaa
    29281 tggcgtcagc atcagcaaga cgtgcgcaat cttaatcagt atcagactcg cggcgcgttc
    29341 gcttatattt ctgaccaaca aaaagtgtac gcccgctttt tctggcagca aaccggccag
    29401 gatcgctacc gtctgctgct cactaaccca ttgggcagca cggaactgga gctgaatgct
    29461 caaccgggta acgtgcagtt agtcgacaat aaaggtcagc gttataccgc cgatgacgcc
    29521 gaagagatga ttggcaaatt gaccggaatg ccaattccgc tcaacagctt gcgccagtgg
    29581 attttaggtt taccgggtga tgcaaccgac tacaaactgg acgaccagta ccgcctgagc
    29641 gaaattacct acagccagaa tggcaaaaac tggaaggttg tttatggtgg ttatgacacc
    29701 aaaacgcaac ctgcgatgcc agccaatatg gaactcaccg acggtggtca acgcatcaag
    29761 ttaaaaatgg ataactggat agtgaaataa tgcggacaca gtggccctct ccggcaaaac
    29821 ttaatctgtt tttatacatt accggtcagc gtgcggatgg ttaccacacg ctgcaaacgc
    29881 tgtttcagtt tcttgattac ggcgacacca tcagcattga gcttcgtgac gatggggata
    29941 ttcgtctgtt aacgcccgtt gaaggcgtgg aacatgaaga taacctgatc gttcgcgcag
    30001 cgcggttgtt gatgaaaact gcggcagaca gcgggcgtct tccgacggga agcggtgcga
    30061 atatcagcat tgacaagcgt ttgccgatgg gcggcggtct cggcggtggt tcatccaatg
    30121 ccgcgacggt cctggtggca ttaaatcatc tctggcaatg cgggctaagc atggatgagc
    30181 tggcggaaat ggggctgacg ctgggcgcag atgttcctgt ctttgttcgg gggcatgccg
    30241 cgtttgccga aggcgttggt gaaatactaa cgccggtgga tccgccagag aagtggtatc
    30301 tggtggcgca ccctggtgta agtattccga ctccggtgat ttttaaagat cctgaactcc
    30361 cgcgcaatac gccaaaaagg tcaatagaaa cgttgctaaa atgtgaattc agcaatgatt
    30421 gcgaggttat cgcaagaaaa cgttttcgcg aggttgatgc ggtgctttcc tggctgttag
    30481 aatacgcccc gtcacgcctg actgggacag gggcctgtgt ctttgctgaa tttgatacag
    30541 agtctgaagc ccgccaggtg ctagagcaag ccccggaatg gctcaatggc tttgtggcga
    30601 aaggcgttaa tctttcccca ttgcacagag ccatgcttta agccgggcaa gctgagtttc
    30661 ggtgacaacg tcaccttgtt ccagacgttg catcgcgctc tttaatacac cgcctggaaa
    30721 ggatcatgcc tggcccgcac agttttcggc agattctttc caccaatgga cgcatgcctg
    30781 aggttcttct cgtgcctgat atgaagcttt ttgctggtaa cgccaccccg gtactagcac
    30841 aacgtattgc caaccgcctg tacacttcac tcggcgacgc cgctgtaggt cgctttagcg
    30901 acggcgaagt cagcgtacaa attaacgaaa atgtacgcgg tggtgatatt ttcatcatcc
    30961 agtccacttg tgcccctact aacgacaacc tgatggaatt agtcgttatg gttgatgccc
    31021 tgcgtcgtgc ttccgcaggt cgtatcaccg ctgttatccc ctactttggc tatgcgcgcc
    31081 aggaccgtcg cgtccgttcc gctcgtgtac caatcactgc gaaagtggtt gcagacttcc
    31141 tctccagcgt cggtgttgac cgtgtgctga cagtggatct gcacgctgaa cagattcagg
    31201 gtttcttcga cgttccggtt gataacgtat ttggtagccc gatcctgctg gaagacatgc
    31261 tgcagctgaa tctggataac ccaattgtgg tttctccgga catcggcggc gttgtgcgtg
    31321 cccgcgctat cgctaagctg ctgaacgata ccgatatggc aatcatcgac aaacgtcgtc
    31381 cgcgtgcgaa cgtttcccag gtgatgcata tcatcggtga cgttgcaggt cgtgactgcg
    31441 tactggtcga tgatatgatc gacactggcg gtacgctgtg taaagctgct gaagcgctga
    31501 aagaacgtgg tgctaaacgt gtatttgcgt acgcgactca cccgatcttc tctggcaacg
    31561 cggcgaacaa cctgcgtaac tctgtaattg atgaagtcgt tgtctgcgat accattccgc
    31621 tgagcgatga aatcaaatca ctgccgaacg tgcgtactct gaccctgtca ggtatgctgg
    31681 ccgaagcgat tcgtcgtatc agcaacgaag aatcgatctc tgccatgttc gaacactaat
    31741 cgaacccggc tcaaagaccc gctgcggcgg gtttttttgt ctgtaatatc catttgtatg
    31801 acctatgcct ccttcacctg ccatttagtt gacagatgat gcgctcacgg atgaaacatt
    31861 attgtgaaca aaatattttc ctcacatgtg atgcctttcc gcgctctgat cgacgtttgc
    31921 tggaaagaaa aatatactgc cgcacggttt acccgtgacc tgattgccgg gataaccgtc
    31981 gggattattg ctatcccgct ggcgatggcg ttggctattg gtagtggtgt ggccccccag
    32041 tacggtttat ataccgcagc tgttgcgggg attgtcattg ctctaacggg tgggtcacgc
    32101 tttagcgttt ccggtccgac tgcggcattt gtggtaattc tctatcccgt ttcgcaacag
    32161 tttggactgg caggactgct ggttgcgacc ttgttgtcgg gaatcttttt gattctgatg
    32221 gggctggcac gctttggtcg tctgattgag tatattccgg tttccgtcac cttaggtttc
    32281 acctcgggta tcgggatcac catcggtacc atgcagatta aagattttct cggtctgcaa
    32341 atggcccatg tcccggaaca ttatctacaa aaagtcggcg cattatttat ggcgctgccg
    32401 accattaatg tgggtgatgc tgccattggc attgtgacgc ttggcattct ggttttctgg
    32461 ccgcgtctgg gcattcgttt acccggtcac cttccggcct tgctggctgg ttgcgcggtg
    32521 atggggattg ttaacctgct cggcggacat gttgctacca tcggttcgca attccactac
    32581 gtcctggccg atggttctca gggtaacggt attccgcaac tgctgccgca actggtgctg
    32641 ccgtgggatc tgcctaattc agaattcacg ctaacctggg attctattcg caccctgctg
    32701 cctgcggcat tctcaatggc aatgctcggg gcgatcgaat ctctgctctg cgctgtggta
    32761 ctggatggta tgaccgggac taagcacaag gcgaatagtg aactggtcgg tcaggggctg
    32821 gggaatatca tcgctccatt ctttggtggt attaccgcta ctgctgccat cgcgcgttct
    32881 gccgctaacg tccgtgccgg ggcaacttcc cctatctcgg cggtgatcca ctctattctg
    32941 gttattctcg ccctgctggt actggcacca ctgctctcct ggctgccgct ttccgccatg
    33001 gcagccctgc tgttgatggt ggcgtggaac atgagtgaag cgcacaaagt ggtcgacttg
    33061 ctgcgtcatg cgccgaaaga tgacatcatc gtcatgctgc tgtgtatgtc actgaccgtg
    33121 ctgtttgata tggttattgc catcagcgtg gggatcgtgc tggcatcgct gctgtttatg
    33181 cgtcgtatcg cacgtatgac tcgcctggca ccggtagtcg tagatgttcc agacgatgtc
    33241 ctggttctgc gcgttattgg cccgctgttt tttgctgctg ctgaaggctt attcacggac
    33301 ctggagtcac gtcttgaagg caaacggatt gtgattctga agtgggatgc cgttccggta
    33361 cttgatgctg gtggtcttga tgcgttccag cgttttgtga agcgtctgcc cgaaggatgt
    33421 gaactgcgcg tgtgcaacgt ggaattccag ccactgcgaa ctatggctcg cgcaggcatt
    33481 caaccgatcc cgggacgcct cgcgttcttc ccgaatcgtc gcgcggcgat ggcggattta
    33541 taagtacgag attgaccagt cagcgcaaac tgactggtca gcaaactgca ttatttgtta
    33601 gctatgacgg cgattatcgc tacggcggca acgtttgtca tagtgacgaa cccaccagta
    33661 acggtcgcac acctgttcat gcccgccaac acgggcaccc gccagccaga gaatggcacc
    33721 gacgaaaata cttagcactg caccatgtgc gaaatactgg ggcatattaa actgtggtaa
    33781 ctggttgagg attgaatacc ccacgccgac caccattacc accagaccca accccatgag
    33841 cacgttaccg agtatcgaag cgtttttgcg tttcatatgt cacctccgga actttctggg
    33901 ttgtaacagg gaatacccct cttccttatg tgtaaagtat agacaacacc ctgtgggtta
    33961 tgtgcgggcg taatcacaat tacaaccctt atttcaacaa aactttacaa ataaacgcct
    34021 gaacaccttg actttctaat agtccactca ggcaattatt acgttatctg catttgatac
    34081 gcagtttttt gtcaagcggg gccgcaacca gtaaactacg cgccagttat gtacacactc
    34141 aggacaaaaa aacgtgacga ttaaattgat tgtcggcctg gcgaaccccg gtgctgaata
    34201 cgccgcaacg cgacataatg ctggtgcctg gttcgttgac ttactggcag agcgtttgcg
    34261 cgctccgctg cgcgaagagg ctaaattctt tggttatact tcgcgagtca cccttggaag
    34321 cgaagatgtc cgcctgttag tcccgactac atttatgaat ctcagcggca aagccgttgc
    34381 ggcgatggcc agttttttcc gcattaatcc ggacgaaatt ctggtggccc acgacgaact
    34441 ggatctgcct cctggcgtcg ccaaatttaa gttgggcggt ggccatggtg gtcacaatgg
    34501 actgaaagac atcatcagta aattgggtaa taaccctaac tttcaccgtt tacgcatcgg
    34561 aatcggtcat ccgggcgata aaaataaagt tgtcggtttt gtgttaggca aaccgcctgt
    34621 tagtgaacag aagttaattg atgaagccat cgacgaagcg gcgcgttgta ctgaaatgtg
    34681 gtttacagat ggcttgacca aagcaacgaa ccgattgcac gcctttaaag cgcaataagt
    34741 cgttgtctgc ggcatttttg ccgagtgccg tgtataatag gcaaagttat ttccatttct
    34801 gcaatctgtt agcaataaca ggttgattat taagatatta aggtgattta aatcatggga
    34861 ttcaaatgcg gtatcgtcgg tttgcccaac gtcgggaaat ctaccctgtt caacgcgctg
    34921 accaaagccg gtattgaagc ggccaacttt ccattctgca ccattgagcc gaacacaggc
    34981 gtcgtaccaa tgcctgaccc tcgcctggat caactggctg aaatcgtaaa accgcaacgt
    35041 acgcttccca cgaccatgga atttgtcgat atcgccggtc tggtaaaagg cgcatcgaaa
    35101 ggcgaaggtc tgggtaacca gttcctgacc aacatccgtg aaaccgaagc gatcggtcac
    35161 gttgttcgct gctttgaaaa tgacaacatc attcacgttt ccggcaaagt taacccggct
    35221 gacgatattg aagttatcaa caccgaactg gcgctggcag acctcgacac ctgcgaacgt
    35281 gcgattcatc gcgtacagaa gaaagccaaa ggtggcgata aagacgcgaa agctgagctg
    35341 gcggtcctgg aaaaatgcct gccccagctg gaaaacgcag gtatgctgcg cgcgctggat
    35401 ttaagcgctg aagagaaagc ggctattcgt tacctgagct tcctgacgct gaaaccaaca
    35461 atgtacatag ccaacgtcaa cgaagatggt tttgaaaaca acccatatct tgaccaggtg
    35521 cgtgaaatcg cggcgaaaga aggttctgtt gtggttccgg tttgtgctgc tgttgaagca
    35581 gacattgccg aactggacga cgaagaacgt gacgagttta tgcaggagct tgggctggaa
    35641 gaaccgggcc tgaaccgtgt gatccgtgcc ggttataagc tgctgaacct gcaaacttac
    35701 ttcaccgctg gggtgaaaga agtgcgtgca tggaccattc cggttggagc aaccgcgccg
    35761 caggcagcgg gcaaaatcca tactgatttt gaaaaaggct ttatccgtgc acaaaccatc
    35821 tcgtttgaag atttcatcac ttacaaaggt gaacaaggcg cgaaagaagc aggcaaaatg
    35881 cgtgcagaag gtaaagatta catcgtgaaa gatggcgatg tgatgaactt ccttttcaac
    35941 gtctaattaa tttattgtct catgaggttt cgctaaatct catgaagagc gcaaaagtat
    36001 ataaaatcca cgcaattgcg tggatttttc atttcaccga gtcctaatca ttcttgtaac
    36061 atcctacact tttgtgctta ttgcctacag tgaaccacga gaataattaa agacataagt
    36121 gttcagatga ctgatatctc attagcagtg atataacgtc agtttttgtt ccactacaca
    36181 tcagccctct tcacagctga tcaatattga tctcgctcca atatgctggc gagaaaagga
    36241 cttacctgaa gggtaatatc ccgcagataa agtccccgaa taacgtgatt acgtgcgatc
    36301 ctggcaagat gaaaatgata accagtagca caataataac gataggccag aaaatccatt
    36361 gaacaaaaat gaatatcgat gttttttgct ttttcttatt agtaatgcaa aaatggcgca
    36421 tattgttttt gttatgcaga tacatcaagc gaaaatccca taactaatta ggattattac
    36481 aaaaaatctt caccaaattt tttaaccgct gtttgttgta atatttttgc catattcata
    36541 gtaacgaatt ggaataagta acgacaactt agggaataac tcgatataaa ctcacctttg
    36601 taaggggggg gttaaatata tattaattcc tgtgttcctg attttgcccg gtaatagaga
    36661 attattctca gagaagctca tcatgattgc accatacaga tatcatgatg ataagaggat
    36721 attattcatt gacttaaaca aaatgacaaa atccatagac ccatagcctg ggtactatat
    36781 aggagtggta tgggcatcaa acaacacaat gggaatacca aagccgatcg tctcgctgaa
    36841 ttaaaaatcc gttcgccctc aattcaactg ataaaatttg gcgctattgg tttgaatgca
    36901 attatctttt cccccctgct gatagctgct gatacaggaa gtcaatatgg caccaatatt
    36961 actattaatg atggtgacag aattacagga gataccgccg atccatcagg aaacctctat
    37021 ggtgtaatga ccccagcagg aaacacgcct ggcaatatca acctgggtaa tgatgtcacc
    37081 gtcaatgtca acgacgcctc tggatatgca aaaggaatca ttattcaggg caaaaacagc
    37141 tccctgacag ctaaccgact cacagtagat gttgttggtc aaacctctgc catcggcatt
    37201 aacttaattg gtgactatac ccatgctgac ttaggcacag gcagcaccat taagagtaac
    37261 gatgacggca tcattattgg gcatagctca acactaacag ccactcaatt caccattgaa
    37321 aactcgaacg gtataggcct aaccatcaat gactatggca ccagtgtcga tcttggaagc
    37381 ggaagtaaaa tcaagaccga tggaagtaca ggtgtttata tcggtggtct caacggcaat
    37441 aacgccaatg gtgctgcgcg ttttacggcg acagacctga caatcgatgt tcagggctac
    37501 agcgccatgg ggataaacgt acagaaaaac tctgttgtcg atctcggaac aaacagtacc
    37561 attaaaacca atggcgataa tgctcacggc ctctggagct ttggccaggt tagcgcgaat
    37621 gcactcactg ttgatgtaac tggagccgcg gccaatggcg tcgaagttcg tggtggtaca
    37681 accactatcg gtgcagatag ccatatttct tccgcgcagg gcggtggcct cgtcaccagt
    37741 agttcagacg cgacaatcaa tttttctggc acggcagcgc aacgaaacag catcttttcc
    37801 ggcggttctt atggtgcctc ggcccagacg gcaacggctg ttatcaacat gcaaaatacc
    37861 gatattacgg ttgatcgtaa tggcagtctg gcgctgggtt tgtgggcgct cagcggcggt
    37921 agaataaccg gagacagttt ggctatcacc ggcgcggcag gagccagagg gatttatgcc
    37981 atgaccaaca gccagatcga cctcacgagc gatctggtca ttgatatgag tacacccgac
    38041 cagatggcca tcgcaacgca acatgacgat ggttatgccg ccagccgcat caacgcctcg
    38101 ggtcgtatgc ttatcaacgg tagcgttctt tccaaaggtg ggctaatcaa tctggatatg
    38161 caccctgggt cggtttggac aggttcctcc ctcagcgata atgtcaatgg cgggaaactg
    38221 gacgttgcaa tgaataacag cgtctggaac gtaacaagta attctaatct cgacacgctg
    38281 gcgctgagcc attcaactgt cgattttgcc agccacgggt caactgccgg cacatttacc
    38341 acattaaacg tagagaacct gagcggtaac agtaccttta ttatgcgtgc tgatgttgtt
    38401 ggcgagggta atggcgttaa taatagaggg gatttattga atatcagcgg gagtagtgct
    38461 ggtaatcacg tattggctat ccgcaaccag ggcagcgagg ccacaacggg aaatgaagtt
    38521 ctgacagtgg taaaaaccac tgacggcgcg gcctcgttca gcgcgtcttc tcaggttgag
    38581 ttggggggat atctgtacga tgtgcgtaaa aatggcacta actgggagct ttacgcttcc
    38641 gggacagttc cggaaccgac tcctaatcct gaacccacac cagctcccgc tcagcctccc
    38701 atagtcaacc ccgatcctac gcctgaaccc gctcccacgc ctaaacccac cacgaccgca
    38761 gatgctggcg gcaattatct caatgtcggt tacttattga actatgttga aaaccgtacg
    38821 ctgatgcaac ggatgggtga cttgcgaaat cagagtaaag acggtaatat ctggttgcgc
    38881 agttatgggg gaagcctgga ctcctttgcc agtggcaaac tgagcggctt tgacatgggt
    38941 tacagcggta tccagtttgg tggggataaa cgtctctctg atgtaatgcc gttgtatgtc
    39001 ggtctgtata ttgactcaac acatgcatcg ccggactata gcggaggcga cggtaccgca
    39061 cgttcagact acatgggaat gtacgccagt tacatggcac aaaacggttt ttacagcgat
    39121 ctcgttataa aagcatcgcg ccagaaaaat agtttccacg tactggacag tcagaacaac
    39181 ggcgttaacg ccaacggcac tgcgaatgga atgagcatct ccctggaagc cgggcagagg
    39241 ttcaacctgt cccctactgg ttatgggttc tatatagagc cgcaaaccca gcttacatac
    39301 agccaccaga atgagatggc tatgaaggcg agtaatggcc tcaatataca tctgaatcac
    39361 tacgaatcgc tgctggggcg tgccagcatg atactggggt atgacatcac cgcaggcaac
    39421 agccagctga atgtctatgt gaagactggc gctatccgcg agttttcagg ggataccgaa
    39481 tatctgttga acgactcccg ggagaagtac agtttcaaag gtaatggctg gaataacggc
    39541 gtgggagtca gtgcacagta taacaaacag cacacattct atctcgaagc ggattacacg
    39601 cagggtaacc tctttgatca gaagcaagtc aacggaggat atcgcttcag cttttaagtg
    39661 cgggatagac aagcccttaa acgatgctga aaattgttag cgtgcgccgg aatgaaatac
    39721 agatcactct gatgtatcag aaatcgtttc tgtctttcat acccggcgct taaactgccc
    39781 tgcatcaatg ccatgttgct tcattttccg ccacagcgta gtgcggccga tcccaagtaa
    39841 agccgacatt tcctgaatgc gaccgcctgt gacctgggct gcgttaataa ttgcctcttt
    39901 ttcaacttcc gcaaatgaca gactggtgga aaggcgggtg gcgctgacat catctgtcgc
    39961 ctgctcggta aacagatgtt caggtaaatc actgacgcga atgcgcccgt tatcactact
    40021 cagagcaaga ttctcgatga cgctgtaaag ttcaaaatcg ttgcctggcc atgcacaaga
    40081 aactaggcga gcgagggcat cgtcatcaat tttcagccgc gtagagaagc gtttttcaag
    40141 actgcgtaat ttgttattca ccagcgccgg aatgctgcca cgccgcatac gcagaggcgg
    40201 gatggtaatt tcaaatgcat gcagcgcgta atacagctgg cgactaaaac gattttgttc
    40261 caccagcatt gcgaggtccg cggtcgttgt tgcaatcact ttgacatcaa ttggtattaa
    40321 acgccgcgca tccagtcgcg tgataacccc ctgcttgata acctgcagca aagcagactg
    40381 taactccacc gccagatatt caatcttttc aagaaacagc gtgccgccgt gtgccagttc
    40441 cagccgactc agacggccat tttcattgtc cgtgcgatcg ccaccaataa attcttccgc
    40501 cagcgcagca tcaccatata actcacaatt gacggcgata taaggacctg cagcacgctc
    40561 gctttcatta tgaattgcct gacttagcag tgccttgccc acgccctctt ctccacaaag
    40621 caggacagga aagctactgc gcgccgcctg gcgaccaaaa tgaatcaagc ggcgggtttg
    40681 cggatcgtcc tgtggcatat gagcgaaggt atggctgact tttcctaatt gactggtcat
    40741 taactgccgc atctgttcca caggatggag caacaaaata aagctggttc cctgcgtttc
    40801 tattatcggt ttaagggtta tcaccgcatc aataaactgg tgctggcttt caaaggttgc
    40861 ttctacgtgt ttgagcggat gtgccagttt tattgcttgt tgcaatacgg cgggtaacgt
    40921 taagagttca gtgattgccc gtccctgact tgccgtcgcg tcaaggcgca agacccgcgc
    40981 cgcctgggca ttaataaatt gcaaattacc ctgctcgtcc cagctaatca cgccatcatc
    41041 catactttct aacagggcat taagttgatt taaatgacgg ttagtttcag cgagcaaact
    41101 gtccgtcagc agtaaattac cgacctcgcg ggcgattgcc aacgtcaacg gcaaatcagc
    41161 tgcggtagtt tgctcaaccg gacacgccag cgctattgtt cccgtcaatc ggcccttgct
    41221 gtcaaacaac ggcgttgcac aaaaggccca gttccagagt gcctgtttga aatgttgatc
    41281 ggccatcgtt ttcacggcct gaccagagat agccgctaac gatagcgcac aagtaccaat
    41341 aattccctcg gcgcaatacg tgccgtcatt gaatcccagt gcacttagct gctgcaaggt
    41401 ttgcggatcg ccattacggc tgagaatgca ggcggtttca tcgaggataa acagcgcaca
    41461 ctctcgcggt gccatatatt cccaggcatc ttccagcgca gcctgcccga gcgtcagcat
    41521 cgctttctta cgccgataaa tagaagcaaa tgtcacgccc tgggcctgat gtggtacgtt
    41581 ccatgtctcc cgtttcatca gcttattgca tcgctcccag gaggttgcaa ttaagggaga
    41641 tatgccccga ccatcgttgt taaaagcgcc actcatatcc cgcatccctt atgacaaaag
    41701 gtgttccggc tcgcaatttt aagtattaat acacgtaaaa gtttcaatat ggaacattca
    41761 ttaaagcata tgtttcacta tgaaacataa tttgggctaa ttttcttttc ccttgcctga
    41821 tgcacaatgg attcatcggc agtcagtggt cgccgtgtcg ttgaacatca tccatgccct
    41881 accgtaattg ctggagcaaa ataatgaaaa aattgatcaa tgatgtgcaa gacgtactgg
    41941 acgaacaact ggcaggactg gcgaaagcgc atccatcgct gacactgcat caggatccgg
    42001 tgtatgtcac ccgagctgat gcccctgttg caggaaaagt cgccctgctg tcgggtggcg
    42061 gcagcggaca cgagccgatg cactgtggtt atatcggtca ggggatgctt tcgggggcct
    42121 gtccgggcga aattttcacc tcaccgacgc ccgataaaat ctttgaatgc tccatgcaaa
    42181 ttgatggcgg cgaaggtgta ctgttgatta tcaaaaatta caccggcgat attcttaact
    42241 ttgaaacagc gaccgagtta ctgcacgata gcggcgtaaa agtgaccact gtggtcattg
    42301 atgacgacgt tgcggtaaaa gacagtcttt ataccgccgg gcgacgcggc gttgccaaca
    42361 ccgtattaat tgaaaaactc gtaggcgcag cggcggagcg tggcgactca ctggacgcct
    42421 gtgcggaact ggggcgtaag ttgaataatc aaggccactc aataggtatc gctctcggtg
    42481 cctgtaccgt tcctgccgcg ggcaaacctt cttttaccct tgcggataat gagatggagt
    42541 ttggcgtagg cattcatggt gagccaggta ttgaccgccg ccctttctct tcccttgatc
    42601 aaaccgtcga tgaaatgttc gacaccctgc tggaacatgg ctcataccat cgcactttgc
    42661 gtttctggga ttatcaacaa ggcagctggc aggaagaacc acaaaccaaa caaccgctcc
    42721 agtctggcga tcgggtgatt gcactggtta acaatcttgg cgcaactccg ctttctgagc
    42781 tgtacggcgt ctataaccgc ctgaccacac gttgccagca agcgggattg actatcgaac
    42841 gtaatttaat tggcgcgtac tgcacctcac tggatatgac cggtttctca atcaccttac
    42901 tgaaagttga tgacgaaacg ctggctctct gggacgcccc ggtccacacc ccggccctta
    42961 actggggtaa ataaggagaa agcaatgtca ctgagcagaa ctcaaattgt taactggctc
    43021 actcgttgtg gcgatatttt caacaccgag agcgagtatc ttaccgggct ggatcgcgag
    43081 attggcgacg ctgaccacgg gctaaatatg aaccgaggct ttagcaaagt ggtggaaaaa
    43141 ctccctgcta tcgcagataa agatatcggt ttcattctca aaaataccgg tatgacgctg
    43201 ctttccagcg tcggtggtgc cagtggtccg ctgttcggta ccttctttat ccgcgccgca
    43261 caggcgaccc aggcacggca aagcctgaca ctggaagagc tttatcagat gttccgcgat
    43321 ggcgcggacg gcgtaatcag tcgcgggaaa gccgaacctg gcgataaaac catgtgtgat
    43381 gtgtgggtgc cggtggtgga atcgttacgt cagtccagcg agcaaaatct ctctgttccg
    43441 gtggcgctcg aagctgccag tagcatcgcc gaatccgctg cacaaagtac gattacgatg
    43501 caagcccgca aaggccgcgc cagttatctc ggtgaacgca gtattggtca ccaggatccc
    43561 ggcgcgacct cggtgatgtt tatgatgcaa atgttggcgt tagccgcaaa agagtaagga
    43621 attggtgatg gtaaacctgg tcatagtttc acatagcagc cgactgggag aaggtgtcgg
    43681 tgaattagcc cgtcagatgt taatgagtga tagttgtaaa atcgccattg ccgcgggaat
    43741 tgacgatcca caaaatccca ttggtaccga tgccgtcaaa gtgatggagg ccatcgaatc
    43801 tgttgctgat gccgaccatg tgctggtcat gatggatatg ggtagcgcat tattgagtgc
    43861 tgaaactgcg ctggaattgc tggctcccga gatcgccgca aaagtacgtt tgtgtgctgc
    43921 gccgttggtc gaaggtacac tggcagcaac ggtcagcgcg gcctcggggg cggatatcga
    43981 caaagttatc tttgacgcta tgcatgcgct ggaagccaaa cgtgaacaac tgggtttacc
    44041 gtcctccgac actgaaatct ctgacacatg tcctccgtac gatgaagaag cccgttctct
    44101 ggcggtggtc ataaaaaacc gtaacggcct gcatgtacgt ccggcctccc ggctggttta
    44161 taccttatcg acatttaatg ccgatatgtt gctggaaaaa aacggcaaat gcgtcacacc
    44221 agagagtatt aaccagattg cgttactaca agttcgctat aacgatacgc tgcgcctgat
    44281 tgcgaaaggg ccagaagctg aagaggcact gatcgctttc cgtcacctgg ctgaagataa
    44341 ctttggtgaa acggaggaag tcgctccacc tactctgcgt cccgttctgc ctgtttcggg
    44401 taaagccttt tattatcaac cagttttatg tacggtacag gcaaaatcaa ccctgaccgc
    44461 ggaagaagaa caagatcgat tacgccaggc tattgacttc acgttattag atctgatgac
    44521 gttaacagcg aaagcagaag ccagcgggct tgacgatatt gccgcaatct tttctggtca
    44581 ccatacactg ttaggtgatc cggaactgct ggcggcggca agcgaactcc ttcagcatga
    44641 acattgcacg gcagaatatg cctggcagca agttcttaaa gaacttagcc agcaatacca
    44701 gcaactggat gatgaatatc tacaagctcg ctatattgat gtggacgatc ttctgcatcg
    44761 caccctggtc cacctgaccc aaacgaaaga agaactcccg cagtttaact cgccaactat
    44821 tctactggcg gagaacattt atccttccac agtactgcaa ctggatccgg cggttgtaaa
    44881 aggtatctgc cttagcgccg gaagtccggt atcccacagc gccctaatcg cccgtgaact
    44941 ggggattggc tggatttgcc agcagggtga gaaactgtat gcgatacaac cagaagaaac
    45001 gctaacgctg gacgttaaaa cgcaacgttt caaccgtcag ggttaagcca ccgttcggat
    45061 ggcatcgttc tgatgtcatc cggcaattta cccagcactg atgtttttta actacctgtt
    45121 atatttttta ccaatttatg atgatttttc ttcgctaatc gcgaaccaaa tgtcgctggc
    45181 tggctaataa acagacgtta tttgatcact ctatttcgcc gttatttatc cctttaattc
    45241 ccttttctaa aatgcctgac cgttcgcaga atgagatttc gatcatgcag ctagtgcgat
    45301 cctgaactaa ggttttctga tacttgaata ccgttttatt cggtttcgcc aaaggagaat
    45361 gattgatgaa atcccccgca ccttctcgcc cgcaaaaaat ggcgttaatt ccagcctgta
    45421 tctttttgtg tttcgctgcg ctatcggtgc aggcagaaga aacaccggta acaccacagc
    45481 cgcctgatat tttattaggg ccgctgttta atgatgtgca aaacgtcaaa ctttttccgg
    45541 accaaaaaac ctttgccgat gccgtgccta acagcgatcc gctgatgatc cttgctgatt
    45601 atcggatgca gcaaaaccag agcggatttg atctgcgcca tttcgttaac gtcaatttca
    45661 ccctgccgaa agaaggcgag aaatatgttc cgccagaggg gcagtcactg cgcgaacata
    45721 ttgacggact ttggccggta ttaacgcgtt ctaccgaaaa caccgaaaaa tgggattctc
    45781 tgttaccgct gccggaacct tatgtcgtgc cgggcggacg ttttcgcgag gtatattact
    45841 gggacaatta cttcaccatg ttaggacttg ccgaaagtgg tcactgggat aaaatcgcgg
    45901 atatggtggc caattttgct catgaaataa acacttacgg tcatattccc aacggcaacc
    45961 gcagttacta tttaagccgc tcgcaaccgc ccttctttgc cctgatggta gagttactgg
    46021 cgcagcatga aggcgatgcc gcgttgaagc aatacctgcc gcaaatgcaa aaagaatatg
    46081 cttactggat ggacggtgtt gaaaacctgc aagccggaca acaggaaaaa cgcgttgtaa
    46141 aacttcagga tagtaccctt ctcaaccgct actgggacga tcgcgatacg ccacgaccag
    46201 agtcatgggt ggaagatatt gccaccgcca aaagcaatcc gaatcgacct gccactgaaa
    46261 tttaccgcga cctgcgctct gccgctgcgt ctggctggga tttcagctcg cgctggatgg
    46321 acaacccgca gcagttaaat accttacgca ccaccagcat cgtaccggtc gatctgaaca
    46381 gcctgatgtt taaaatggaa aaaatcctcg cccgcgccag caaagctgca ggagataacg
    46441 cgatggcaaa ccagtacgaa acgctggcga atgcccgtca aaaagggatc gaaaaatacc
    46501 tgtggaacga tcaacaaggc tggtatgccg attacgacct gaaaagtcat aaagtgcgca
    46561 atcagttaac cgcggccgcc ctgttcccgc tgtacgtcaa tgcggcagcg aaagatcgcg
    46621 ccaacaaaat ggcgacggcg acgaaaacac atctgctgca acccggcggc ctgaacacca
    46681 cgtcggtgaa aagtgggcaa caatgggatg cgccaaatgg ctgggcaccg ttacagtggg
    46741 tcgcgacaga aggattacaa aactacgggc aaaaagaggt ggcgatggac attagctggc
    46801 acttcctgac caatgttcag cacacctatg accgggagaa aaagctggtg gaaaaatatg
    46861 atgtcagcac caccggaacg gggggcggcg gtggcgaata tccattacag gatggctttg
    46921 gctggaccaa tggcgtgacg ctgaaaatgc tggatttgat ctgcccgaaa gagcaaccgt
    46981 gtgacaatgt tccggcgacg catccgaccg ttaagtcagc aacgacgcaa ccctcaacca
    47041 aagaggcaca acccacacct taaccagcgc ttactccgac aacgtcattc ggcttctccg
    47101 gatggcgttg tcggcagata acaccatatc tatggttttt tctacacaga cggtttttca
    47161 agtcctggcg ggaagtattg cgcaaatgtt tgccatacgt ggttgccgaa gatagcgtca
    47221 tttctacggg taagtgtttc actttcatca cccggcgatt tacgccgacg ttggctatat
    47281 aaagcatcaa ttgctaccga atattgttac ggtgttcact catcaacgtt tttttgcttt
    47341 ctggagttaa attaataatc acataatcca gacactccag agaatttagc aaccaaaaaa
    47401 agaattcaga ccattcctct tcgcgtaact gaagcattgc cgaacgggta ttttggctta
    47461 atctgccaga aggattgatc aattcta
//
LOCUS       DAJTRC010000022        47289 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.022, whole genome shotgun sequence.
ACCESSION   DAJTRC010000022 DAJTRC010000000
VERSION     DAJTRC010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 47289)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47289)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47289
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.022"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..480
                     /gene="sseA"
                     /locus_tag="OO357_001335"
     CDS             <1..480
                     /gene="sseA"
                     /locus_tag="OO357_001335"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417016.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HCR5886338.1"
                     /translation="LAGWQRDDLLLEEGAVELPEGEFNAAFNPEAVVKVTDVLLASHE
                     NTAQIIDARPAARFNAEVDEPRPGLRRGHIPGALNVPWTELVREGELKTTDELDAIFF
                     GRGVSYDKPIIVSCGSGVTAAVVLLALATLDVPNVKLYDGAWSEWGARADLPVEPVK"
     gene            complement(1299..2075)
                     /gene="sseB"
                     /locus_tag="OO357_001336"
     CDS             complement(1299..2075)
                     /gene="sseB"
                     /locus_tag="OO357_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708361.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enhanced serine sensitivity protein SseB"
                     /protein_id="HCR5886339.1"
                     /translation="MSETKNELEDLLEKAATEPAHRPAFFRTLLESTVWVPGTAAQGE
                     AVVEDSALDLQHWEKEDGTSVIPFFTSLEALQQAVEDEQAFVVMPVRTLFEMTLGETL
                     FLNAKLPTGKEFMPREISLLIGEEGNPLSSQEILEGGESLILSEVAEPPAQMIDSLTT
                     LFKTIKPVKRAFICSIKENEEAQPNLLIGIEADGDIEEIIQATGSVATDTLPGDEPID
                     ICQVKKGEKGISHFITEHIAPFYERRWGGFLRDFKQNRII"
     gene            complement(2217..3500)
                     /gene="pepB"
                     /locus_tag="OO357_001337"
     CDS             complement(2217..3500)
                     /gene="pepB"
                     /locus_tag="OO357_001337"
                     /EC_number="3.4.11.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase PepB"
                     /protein_id="HCR5886340.1"
                     /translation="MTEAMKITLSTQPADARWGEKATYSINNDGITLHLNGADDLGLI
                     QRAARKIDGLGIKHVQLSGEGWDADRCWAFWQGYKGPKGTRKVEWPDLDDAQRQELDN
                     RLMIIDWVRDTINAPAEELGPSQLAQRAVDLISNVAGDRVTYRITKGEDLREQGYMGL
                     HTVGRGSERSPVLLALDYNPTGDKEAPVYACLVGKGITFDSGGYSIKQTAFMDSMKSD
                     MGGAATVTGALAFAIMRGLNKRVKLFLCCADNLISGNAFKLGDIITYRNGKKVEVMNT
                     DAEGRLVLADGLIDASAQKPEMIIDAATLTGAAKTALGNDYHALFSFDDALAGRLLAS
                     ASQENEPFWRLPLAEFHRSQLPSNFAELNNTGSAAYPAGASTAAGFLSHFVENYQQGW
                     LHIDCSATYRKAPVEQWSAGATGLGVRTIANLLTA"
     gene            complement(3559..3759)
                     /gene="iscX"
                     /locus_tag="OO357_001338"
     CDS             complement(3559..3759)
                     /gene="iscX"
                     /locus_tag="OO357_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004104502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein IscX"
                     /protein_id="HCR5886341.1"
                     /translation="MGLKWTDSREIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDD
                     DPQASNEKILEAILLVWLDEAE"
     gene            complement(3771..4106)
                     /gene="fdx"
                     /locus_tag="OO357_001339"
     CDS             complement(3771..4106)
                     /gene="fdx"
                     /locus_tag="OO357_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015683702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISC system 2Fe-2S type ferredoxin"
                     /protein_id="HCR5886342.1"
                     /translation="MPKIVILPHQDLCPDGAVLEANSGETILDAALRNGIEIEHACEK
                     SCACTTCHCIVREGFDSLPESSEQEDDMLDKAWGLEPESRLSCQARVTDEDLVVEIPR
                     YTINHAREH"
     gene            complement(4108..5958)
                     /gene="hscA"
                     /locus_tag="OO357_001340"
     CDS             complement(4108..5958)
                     /gene="hscA"
                     /locus_tag="OO357_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001559634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S protein assembly chaperone HscA"
                     /protein_id="HCR5886343.1"
                     /translation="MALLQISEPGLSAAPHQRRLAAGIDLGTTNSLVATVRSGQAETL
                     ADHEGRHLLPSVVHYQQQGHSVGYDARTNAALDTANTISSVKRLMGRSLADIQQRYPH
                     LPYQFQASENGLPMIETAAGLLNPVRVSADILKALAARATEALAGELDGVVITVPAYF
                     DDAQRQGTKDAARLAGLHVLRLLNEPTAAAIAYGLDSGQEGVIAVYDLGGGTFDISIL
                     RLSRGVFEVLATGGDSALGGDDFDHLLADYIREQADIPDRSDNRVQRELLDATIAAKI
                     ALSDADSVTVNVAGWQGEISREQFNELIAPLVKRTLLACRRALKDAGVEADEVLEVVM
                     VGGSTRVPLVRERVGEFFGRPPLTSIDPDKVVAIGAAIQADILVGNKPDSEMLLLDVI
                     PLSLGLETMGGLVEKVIPRNTTIPVARAQDFTTFKDGQTAMSIHVMQGERELVQDCRS
                     LARFALRGIPALPAGGAHIRVTFQVDADGLLSVTAMEKSTGVEASIQVKPSYGLTDSE
                     IASMIKDSMSYAEQDVKARMLAEQKVEAARVLESLHGALAADAALLSAAERQVIDNAA
                     AHLSEVAQGDDVDAIEQAIKNVDKQTQDFAARRMDQSVRRALKGHSVDEV"
     gene            complement(5975..6490)
                     /gene="hscB"
                     /locus_tag="OO357_001341"
     CDS             complement(5975..6490)
                     /gene="hscB"
                     /locus_tag="OO357_001341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone HscB"
                     /protein_id="HCR5886344.1"
                     /translation="MDYFTLFGLPARYQLDTQALSLRFQDLQRQYHPDKFASGSLAEQ
                     LAAVQQSATINQAWQTLRHPLMRAEYLLSLHGFDLASEQHTVRDTAFLMEQLELREEL
                     DEIEQAKDEARLESFIKRVKKMFDTRHQLMVEQLDNEAWDAAADTVRKLRFLDKLRSS
                     AEQLEEKLLDF"
     gene            complement(6586..6909)
                     /gene="iscA"
                     /locus_tag="OO357_001342"
     CDS             complement(6586..6909)
                     /gene="iscA"
                     /locus_tag="OO357_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly protein IscA"
                     /protein_id="HCR5886345.1"
                     /translation="MSITLSDSAAARVNTFLANRGKGFGLRLGVRTSGCSGMAYVLEF
                     VDEPTPEDIVFEDKGVKVVVDGKSLQFLDGTQLDFVKEGLNEGFKFTNPNVKDECGCG
                     ESFHV"
     gene            complement(6926..7312)
                     /gene="iscU"
                     /locus_tag="OO357_001343"
     CDS             complement(6926..7312)
                     /gene="iscU"
                     /locus_tag="OO357_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold IscU"
                     /protein_id="HCR5886346.1"
                     /translation="MAYSEKVIDHYENPRNVGSFDNNDENVGSGMVGAPACGDVMKLQ
                     IKVNDEGIIEDARFKTYGCGSAIASSSLVTEWVKGKSLDEAQAIKNTDIAEELELPPV
                     KIHCSILAEDAIKAAIADYKSKREAK"
     gene            complement(7340..8554)
                     /gene="iscS"
                     /locus_tag="OO357_001344"
     CDS             complement(7340..8554)
                     /gene="iscS"
                     /locus_tag="OO357_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017459011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HCR5886347.1"
                     /translation="MKLPIYLDYSATTPVDPRVAEKMMQFMTMDGTFGNPASRSHRFG
                     WQAEEAVDIARNQIADLVGADPREIVFTSGATESDNLAIKGAANFYQKKGKHIITSKT
                     EHKAVLDTCRQLEREGFEVTYLAPQRNGIIDLKELEAAMRDDTILVSIMHVNNEIGVV
                     QDIAAIGEMCRARGIIYHVDATQSVGKLPIDLSQLKVDLMSFSGHKIYGPKGIGALYV
                     RRKPRVRIEAQMHGGGHERGMRSGTLPVHQIVGMGEAYRIAKEEMATEMERLRGLRNR
                     LWNGIKDIEEVYLNGDLEHGAPNILNVSFNYVEGESLIMALKDLAVSSGSACTSASLE
                     PSYVLRALGLNDELAHSSIRFSLGRFTTEEEIDYTIELVRKSIGRLRDLSPLWEMYKQ
                     GVDLNSIEWAHH"
     gene            complement(8666..9154)
                     /gene="iscR"
                     /locus_tag="OO357_001345"
     CDS             complement(8666..9154)
                     /gene="iscR"
                     /locus_tag="OO357_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002913993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly transcriptional regulator
                     IscR"
                     /protein_id="HCR5886348.1"
                     /translation="MRLTSKGRYAVTAMLDVALNSEAGPVPLADISERQGISLSYLEQ
                     LFSRLRKNGLVSSVRGPGGGYLLGKDASSIAVGEVISAVDESVDATRCQGKGGCQGGD
                     KCLTHALWRDLSDRLTGFLNNITLGELVNNQEVLDVSGRQHTHDAPRTRTQDAIDVKL
                     RA"
     gene            complement(9424..10164)
                     /gene="trmJ"
                     /locus_tag="OO357_001346"
     CDS             complement(9424..10164)
                     /gene="trmJ"
                     /locus_tag="OO357_001346"
                     /EC_number="2.1.1.200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (cytosine(32)/uridine(32)-2'-O)-methyltransferase TrmJ"
                     /protein_id="HCR5886349.1"
                     /translation="MLQNIRIVLVETSHTGNMGSVARAMKTMGLTNLWLVNPLVKPDS
                     QAIALAAGASDVIGNAHIVDTLDEALAGCSLVVGTSARSRTLPWPMLDPRECGLKSVA
                     EAANTPVALVFGRERVGLTNEELQKCHYHVAIAANPEYSSLNLAMAVQVIAYEVRMAW
                     LATQENGEQVEHEETPYPLVDDLERFYGHLEQTLLATGFIRENHPGQVMNKLRRLFTR
                     ARPESQELNILRGILASIEQQNKGNKAE"
     gene            10283..11086
                     /gene="suhB"
                     /locus_tag="OO357_001347"
     CDS             10283..11086
                     /gene="suhB"
                     /locus_tag="OO357_001347"
                     /EC_number="3.1.3.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-1-monophosphatase"
                     /protein_id="HCR5886350.1"
                     /translation="MHPMLNIAVRAARKAGNLIAKNYETPDAVEASQKGSNDFVTNVD
                     KAAEAVIIDTIRKSYPQHTIITEESGELEGTDQDVQWVIDPLDGTTNFIKRLPHFAVS
                     IAVRIKGRTEVAVVYDPMRNELFTATRGQGAQLNGYRLRGSTARDLDGTILATGFPFK
                     AKQYATTYINIVGKLFNECADFRRTGSAALDLAYVAAGRVDGFFEIGLRPWDFAAGEL
                     LVREAGGIVSDFTGGHNYMLTGNIVAGNPRVVKAILANMRDELSDALKH"
     gene            11231..12085
                     /locus_tag="OO357_001348"
     CDS             11231..12085
                     /locus_tag="OO357_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5886351.1"
                     /translation="MALPVNKRVPKILFILFVVAFCVYLVPRVAINFFYYPDDKIYGP
                     DPWSAESVEFTAKDGTRLQGWFIPSSTGPADNAIATIIHAHGNAGNMSAHWPLVSWLP
                     ERNFNVFMFDYRGFGKSKGTPSQAGLLDDTQSAINVVRHRSDVNPQQLVLFGQSIGGA
                     NILAVIGQGDREGIRAVILDSTFASYATIANQMIPGSGYLLDESYSGENYIASVSPIP
                     LLLIHGKADHVIPWQHSEKLYSLAKEPKRLILIPDGEHIDAFSDRHGNVYREQMVDFI
                     LSALNPQN"
     gene            12276..13556
                     /gene="csiE"
                     /locus_tag="OO357_001349"
     CDS             12276..13556
                     /gene="csiE"
                     /locus_tag="OO357_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stationary phase inducible protein CsiE"
                     /protein_id="HCR5886352.1"
                     /translation="MMPTLAPPSVLSAPQRRCQILLTLFQPGLTATMATFSELNGVDD
                     DIASLDISETGREILRYHQLTLTTGYDGSYRVEGTVLNQRLCLFHWLRRGFRLCPSFI
                     TSQFTPALKSELKRRGIARNFYDDTNLQALVNLCSRRLQKRFESRDIHFLCLYLQYCL
                     LQHHAGITPQFNPLQRRWAESCLEFQVAQEIGRHWQRRALQHVPPDEPLFMALLFSML
                     RVPDPLRDAHQRDRQLRQSIKRLVNHFRELGNVRFYDEQGLCDQLYIHLAQALNRSLF
                     AIGIDNTLPEEFARLYPRLVRTTRAALAGFESEYGVHLSDEESGLVAVIFGAWLMQEN
                     DLHEKQIILLTGNDSEREAQIEQQLRELTLLPLNIKHMSVKAFLQTGAPRGAALIIAP
                     YTMPLPLFSPPLIYTDLTLTTHQQEQIRKMLESA"
     gene            complement(13548..14687)
                     /locus_tag="OO357_001350"
     CDS             complement(13548..14687)
                     /locus_tag="OO357_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate MFS transporter"
                     /protein_id="HCR5886353.1"
                     /translation="MVLQSTRWLALGYFTYFFSYGIFLPFWSVWLKGIGLTPETIGLL
                     LGAGLVARFLGSLLIAPRVSDPSRLISALRVLALLPLLFAVAFWAGAHVAWLMLVMIG
                     FNLFFSPLVPLTDALANTWQKQFPLDYGKVRLWGSVAFVIGSALTGKLVTMFDYRVIL
                     VLLTLGVASMLLGFLIRPTIQPQGASRQQESTGWSAWLALVRQNWRFLACVCLLQGAH
                     AAYYGFSAIYWQAAGYSASAVGYLWSLGVVAEVIIFALSNKLFRRCSARDMLLISAIC
                     GVVRWGIMGATTALPWLIVVQILHCGTFTVCHLAAMRYIAARQVSEVIRLQAVYSAVA
                     MGGSIAIMTVFAGFLYQYLGHGVFWVMALVALPAMFLRPKVVPSC"
     gene            complement(14847..15737)
                     /gene="hcaR"
                     /locus_tag="OO357_001351"
     CDS             complement(14847..15737)
                     /gene="hcaR"
                     /locus_tag="OO357_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HcaR"
                     /protein_id="HCR5886354.1"
                     /translation="MELRHLRYFVAVAQALNFTRAAEKLHTSQPSLSSQIRDLENCVG
                     VPLLVRDKRKVALTAAGECFLQDALAILEQAENAKLRARKIVQEDRQLTIGFVPSAEV
                     NLLPKVLPMFRLRQPDTLIELVSLITTQQEEKIRRGELDVGLMRHPVYSPEIDYLELF
                     DEPLVVVLPVDHPLAHEKEITAAQLDGVNFVSTDPAYSGSLAPIVKAWFAQENSQPNI
                     VQVATNILVTMNLVGMGLGVTLIPGYMNNFNTGQVVFRPIAGNVPSIALLMAWKKGEM
                     KLALRDFIAIVQERLASVTA"
     gene            15873..17234
                     /locus_tag="OO357_001352"
     CDS             15873..17234
                     /locus_tag="OO357_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HCR5886355.1"
                     /translation="MTTPSDLNIYQLIDTQNGRVTPRIYTDPDIYQLELERIFGRCWL
                     FLAHESQIPKPGDFFNTYMGEDAVVVVRQKDGSIKAFLNQCRHRAMRVSYADCGNSRA
                     FTCPYHGWSYGINGELIDVPLEPRAYPQGLCKSHWGLNEVPCVESYKGLIFGNWDTSA
                     PGLHDYLGDIAWYLDGMLDRREGGTEIVGGVQKWVINCNWKFPAEQFASDQYHALFSH
                     ASAVQVLGAKDDGSDKRLGDGQTARPVWETAKDALQFGQDGHGSGFFFTEKPDANVWV
                     DGAVSSYYRETYAEAEQRLGEVRALRLAGHNNIFPTLSWLNGTATLRVWHPRGPDQVE
                     VWAFCITDKAASDEVKAAFENSATRAFGPAGFLEQDDSENWCEIQKLLKGHRARNSKL
                     CLEMGLGQEKRRDDGIPGITNYIFSETAARGMYQRWADLLSSESWQEVLDKTAAYQQE
                     VMK"
     gene            17231..17749
                     /locus_tag="OO357_001353"
     CDS             17231..17749
                     /locus_tag="OO357_001353"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate/cinnamic acid dioxygenase
                     subunit beta"
                     /protein_id="HCR5886356.1"
                     /translation="MSAQVSLELHHRISQFLFHEASLLDDWKFRDWLAQLDEEIRYTM
                     RTTVNAQTRDRRKGVQPPTTWIFNDTKDQLERRIARLETGMAWAEEPPSRTRHLISNC
                     QVSETDIPNVFAVRVNYLLYRAQKERDETFYVGTRFDKVRRLEDDNWRLLERDIVLDQ
                     AVITSHNLSVLF"
     gene            17749..18069
                     /locus_tag="OO357_001354"
     CDS             17749..18069
                     /locus_tag="OO357_001354"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-phenylpropionate/cinnamic acid
                     dioxygenase ferredoxin subunit"
                     /protein_id="HCR5886357.1"
                     /translation="MNRIYACPVADVPEGEALRIDTSPVIALFNVGGEFYAINDRCSH
                     GNASMSEGYLEDDATVECPLHAASFCLKTGKALCLPATDPLTTYPVHVEGGDIFIDLP
                     EAQP"
     gene            18066..18878
                     /gene="hcaB"
                     /locus_tag="OO357_001355"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
     CDS             18066..18878
                     /gene="hcaB"
                     /locus_tag="OO357_001355"
                     /gene_synonym="phdD"
                     /gene_synonym="yfhX"
                     /EC_number="1.3.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phenylpropionate-dihydrodiol/cinnamic
                     acid-dihydrodiol dehydrogenase"
                     /protein_id="HCR5886358.1"
                     /translation="MSDLHNESIFITGGGSGLGLALVERFIEEGAQVATLELSAAKVA
                     SLRQRFGEHILAVEGNVTCYADYQRAVDQILTRSGKLDCFIGNAGIWDHNASLVNTPA
                     ETLETGFHELFNVNVLGYLLGAKACAPALIASEGSMIFTLSNAAWYPGGGGPLYTTSK
                     HAATGLIRQLAYELAPKVRVNGVGPCGMASDLRGPQALGQSETSIMQSLTPEKIAAIL
                     PLQFFPQPADFTGPYVMLASRRNNRALSGVMINADAGLAIRGIRHVAAGLDL"
     gene            18888..20105
                     /locus_tag="OO357_001356"
     CDS             18888..20105
                     /locus_tag="OO357_001356"
                     /EC_number="1.14.12.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylpropionate dioxygenase ferredoxin
                     reductase subunit"
                     /protein_id="HCR5886359.1"
                     /translation="MKEKTIIIVGGGQAAAMAAASLRQQGFTGELHLFSDERHLPYER
                     PPLSKSMLLEDSPQLQQVLPANWWQENNVHLHSGVTIKTLGRDTRELVLTNGESWHWD
                     QLFIATGAAARPLPLLDALGERCFTLHHAGDAARLREVLQPERSVVIVGAGTIGLELA
                     ASATQRSAAQRRCKVTVIELAATVMGRNAPPPVQRYLLQRHQQAGVRILLNNAIEHVV
                     DGEKVELTLQSGETLQADVVIYGIGISANEQLAREANLDTANGIVIDEACRTCDPAIF
                     AGGDVAITRLDNGALHRCESWENANNQAQIAAAAMLGLPLPLLPPPWFWSDQYSDNLQ
                     FIGDMRGDDWLCRGNPETQKAIWFNLQNGVLIGAVTLNQGREIRPIRKWIQSGKTFDA
                     KLLIDENIALKSL"
     gene            20202..20624
                     /locus_tag="OO357_001357"
     CDS             20202..20624
                     /locus_tag="OO357_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417038.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5886360.1"
                     /translation="MNTLRYFDFGAARPVLLLIARIAVVLIFIIFGFPKMMGFDGTVQ
                     YMASLGAPMPMLAAIIAVVMEVPAAILIVLGFFTRPLAVLFIFYTLGTAVIGHHYWDM
                     TGDAVGPNMINFWKNVSIAGAFLLLAITGPGAISLDRR"
     gene            complement(20672..21544)
                     /locus_tag="OO357_001358"
     CDS             complement(20672..21544)
                     /locus_tag="OO357_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HCR5886361.1"
                     /translation="MTIYTLSHGSLKLDVSDQGGVIEGFWRDTTPLLRPGKKSGVATD
                     ASCFPLVPFANRVSGNRFVWQGREYQLQPNVEWDAHYLHGDGWLGEWQCVSHSDDSLC
                     LVYEHRSGVYHYRVSQAFHLTADTLTVTLSVTNQGAETLPFGTGWHPYFPLSPQTRIQ
                     AQASGYWLEREQWLAGEFCEQLPQELDFNQLAPLPRQWVNNGFAGWNGQARIEQPQEG
                     YAIIMETTPPAPCYFIFVSDPAFDKGYAFDFFCLEPMSHAPDEHHRPEGGDLIALAPG
                     ESTTSEMSLRVEWL"
     gene            complement(21556..22650)
                     /locus_tag="OO357_001359"
     CDS             complement(21556..22650)
                     /locus_tag="OO357_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5886362.1"
                     /translation="MKINRIVNEGFMKTMLAAYLLGNSTVDLREVAVPTPGINQVLIK
                     MKSSGICGSDVHYIYHQHRATAAAPDKPLYQGFINGHEPCGQIVAMGQGCRHFKEGDR
                     VLVYHISGCGFCPNCRRGFPISCTGKGKAAYGWQRDGGHAEYLLAEEKDLILLPDALS
                     YEDGAFISCGVGTAYEGILRGEVSGSDNVLVVGLGPVGMMAMMLAKGRGAKRIIGVDM
                     LPERLAMAKQLGGMDHGYLATTEGLPQIIAELTHGGADVALDCSGNAAGRLLALQSTA
                     DWGRVVYIGETGKVEFEVSADLMHHQRRIIGSWVTSLFHMEKCAHDLTDWKLWPRNAI
                     THRFSLEQAGDAYVLMASGKCGKVVINFPD"
     gene            complement(22683..23681)
                     /locus_tag="OO357_001360"
     CDS             complement(22683..23681)
                     /locus_tag="OO357_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5886363.1"
                     /translation="MYASSLPLPQGKSVSLKQFVSRHINEIGLLVVIAILYLVFSLNA
                     PGFISLNNQMNVLRDAATIGIAAWAMTLIIISGEIDVSVGPMVAFVSVCLAFLLQFEV
                     PLAIACLLVLLLGALMGTLAGVLRGVFNVPSFVATLGLWSALRGMGLFMTNALPVPID
                     ENEVLDWLGGQFLGVPVSALIMMVLFALFVFISRKTAFGRSVFAVGGNATAAQLCGIN
                     VRRVRILIFTLSGLLAAVTGILLAARLGSGNAGAANGLEFDVIAAVVVGGTALSGGRG
                     SLFGTLLGVLVITLIGNGLVLLGINSFFQQVVRGVIIVVAVLANILLTQRSSKAKR"
     gene            complement(23706..25217)
                     /locus_tag="OO357_001361"
     CDS             complement(23706..25217)
                     /locus_tag="OO357_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5886364.1"
                     /translation="MFTATEAVPVAKVVAGNKRYPGVVALDNVNFTLNKGEVRALLGK
                     NGAGKSTLIRMLTGSERPDSGDIWIGETRLEGDEATLTRRAAELGVRAVYQELSLVEG
                     LTVAENLCLGQWPRRNGMIDYLQMAQDAQRCLQALGVDVSPEQLVSTLSPAQKQLVEI
                     ARVMKGEPRVVILDEPTSSLASAEVELVISAVKKMSALGVAVIYVSHRMEEIRRIASY
                     ATVMRDGQVAGDVMLENTSTHHIVSLMLGRDHVDIAPVAPQEIVDQAVLEVRALRHKP
                     KLEDISFTLRRGEVLGIAGLLGAGRSELLKAIVGLETYEQGEIVINGEKIMRPDYGDM
                     LKRGIGYTPENRKEAGIIPWLGVDENTVLTNRQKISTNGVLQWSTIRRLTEEVMQRMT
                     VKAASSETPIGTLSGGNQQKVVIGRWVYAASQILLLDEPTRGVDIEAKQQIYRIVREL
                     AAEGKSVVFISSEVEELPLVCDRILLLQHGTFSQEFHSPVNVDELMSAILSVH"
     gene            complement(25240..26222)
                     /locus_tag="OO357_001362"
                     /pseudo
     CDS             complement(25240..26222)
                     /locus_tag="OO357_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            complement(26319..29420)
                     /locus_tag="OO357_001363"
     CDS             complement(26319..29420)
                     /locus_tag="OO357_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000266164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5107 domain-containing protein"
                     /protein_id="HCR5886365.1"
                     /translation="MWLENDYIKVMILPELGGRVHRAWDKVKQRDFVYHNEVIKPALV
                     GMLGPWISGGIEFNWPQHHRPTTFMPVDFTLEAHEDGAQTVWVGETEPMHGLQVMTGF
                     TLRPDRAALEIASRVYNGNATPRHFLWWVNPAVKGGEGHQSVFPPDVTAVFDHGKRAV
                     SAFPIATGTYYKVDYSAGVDISRYKNVPVPTSYMAEKSQYDFVGAWCHDEDGGLLHVA
                     NHHIAPGKKQWSWGHSEFGQAWDKSLTDNNGPYIELMTGIFADNQPDFTWLDAYEEKR
                     FEQYFLPYHSLGMVQNASRDAVIKLQRSERGIEWGLYAISPLNGYRLAIREIGKCNAL
                     LDDAVALTPATAIQGVLHGINPDRLTIELSDADGNIVLSYQEHQPQELPLPDVAKAPL
                     AAQDITSTDEAWFIGQHLEQYHHASRSPFDYYLRGVALDPLDYRCNLALAMLEYNRAD
                     FPQAVAYASQALKRAHALNKKPQCGQASLIRASAYERQGQYQQAEEDFWRAVWSGNSK
                     AGGYYGLARLAARNDNFDAGLDFCQQSLRACPTNQEVLCLHNLLLVLSGRQDNARLQR
                     EKLLRDYPLNATLWWLNWFDGRSESALAQWRGLCQGRDVNALMTAGQLINWGMPTLAA
                     EMLNALDCQRTLPLYLQASLLPKAERGELVAKAIDVFPQFVRFPNTLEEVAALESIEE
                     CWFARHLLACFYYNKRSYNKAIALWQRCVEMSPEFADGWRGLAIHAWNKKHDYELAAR
                     YLDNAYQLAPQDARLLFERDLLDKLSGATPEKRLARLENNLEIALKRDDMTAELLNLW
                     HLTGQADKAADILATRKFHPWEGGEGKVTSQFILNQLLRAWQHLDAREPQQASELLHA
                     ALYYPENLSEGRLPGQTDNDIWFWQAICANAQGDETEAMRCLRLAATGDRTINIHSYY
                     NDQPVDYLFWQGMALRLLGEQQTAQQLFSEMKQWAQEMAKTSIEADFFAVSQPDLLSL
                     YGDLQQQHKEKCLMVAMLASAGLGEVAQYESARAELTAINPAWPKAALFTTVMPFIFN
                     YVH"
     gene            complement(29427..29600)
                     /locus_tag="OO357_001364"
     CDS             complement(29427..29600)
                     /locus_tag="OO357_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000189282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886366.1"
                     /translation="MTPVKVWQERVEIPTYETGPQDIHPMFLENRVYQGSSGAVYPYG
                     VTDTLSEQKTLKS"
     gene            29718..30911
                     /locus_tag="OO357_001365"
     CDS             29718..30911
                     /locus_tag="OO357_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HCR5886367.1"
                     /translation="MRACINNQQIRHHNKCVILELLYRQKRANKSTLARLAQISIPAV
                     SNILQELESEKRVVNIDDESQTRGHSSGTWLIAPEGDWTLCLNVTPTSIECQVANACL
                     SPKGEFEYLQIDVPTPQALLSEIEKCWHRHRKLWPDRTINLALAIHGQVDPVTGVSQT
                     MPQAPWTTPVEVKYLLEEKLGIRVLVDNDCVMLALAEKWQNNSQERDFCVINVDYGIG
                     SSFVINEQIYRGSLYGSGQIGHTIVNPDGVVCDCGRYGSLETVASLSALKKQARVWLK
                     SQPVNTQLEPEKLTTAQLIAAWQSGEPWITSWVDRSANAIGLSLYNFLNILNINQIWL
                     YGRSCAFGENWLNTIIRQTGFNPFDRDEGPSVKATQIGFGQLSRAQQVLGIGYLYVEA
                     QLRQI"
     gene            complement(31109..32362)
                     /gene="glyA"
                     /locus_tag="OO357_001366"
     CDS             complement(31109..32362)
                     /gene="glyA"
                     /locus_tag="OO357_001366"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HCR5886368.1"
                     /translation="MLKREMNIADYDAELWQAMEQEKVRQEEHIELIASENYTSPRVM
                     QAQGSQLTNKYAEGYPGKRYYGGCEYVDIVEQLAIDRAKELFGADYANVQPHSGSQAN
                     FAVYTALLEPGDTVLGMNLAHGGHLTHGSPVNFSGKLYNIVPYGIDATGHIDYADLEK
                     QAKEHKPKMIIGGFSAYSGVVDWAKMREIADSIGAYLFVDMAHVAGLVAAGVYPNPVP
                     HAHVVTTTTHKTLAGPRGGLILAKGGSEELYKKLNSAVFPGGQGGPLMHVIAGKAVAL
                     KEAMEPEFKTYQQQVAKNAKAMVEVFLERGYKVVSGGTDNHLFLVDLVDKNLTGKEAD
                     AALGRANITVNKNSVPNDPKSPFVTSGIRVGTPAITRRGFKEAEAKELAGWMCDVLDS
                     INDEAVIERIKGKVLDICARYPVYA"
     gene            32689..33879
                     /gene="hmpA"
                     /locus_tag="OO357_001367"
     CDS             32689..33879
                     /gene="hmpA"
                     /locus_tag="OO357_001367"
                     /EC_number="1.14.12.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NO-inducible flavohemoprotein"
                     /protein_id="HCR5886369.1"
                     /translation="MLDAQTIATVKATIPLLVETGPKLTAHFYDRMFTHNPELKEIFN
                     MSNQRNGDQREALFNAIAAYASNIENLPALLPAVEKIAQKHTSFQIKPEQYNIVGEHL
                     LATLDEMFSPGQEVLDAWGKAYGVLANVFINREAEIYNENASKAGGWEGTRDFRIVAK
                     TPRSALITSFELEPVDGGAVAEYRPGQYLGVWLKPEGFPHQEIRQYSLTRKPDGKGYR
                     IAVKREEGGQVSNWLHNHANVGDVVKLVAPAGDFFMAVADDTPVTLISTGVGQTPMLA
                     MLDTLAKAGHTAQVNWFHAAENGEVHAFADEVKELGQSLPRFTAHTWYRQPSEADRAK
                     GQFDSEGLMDLSKLEGAFSDPTMQFYLCGPVGFMQFTAKQLVDLGVKQENIHYECFGP
                     HKVL"
     gene            complement(33924..34262)
                     /gene="glnB"
                     /locus_tag="OO357_001368"
     CDS             complement(33924..34262)
                     /gene="glnB"
                     /locus_tag="OO357_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017373484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulatory protein P-II"
                     /protein_id="HCR5886370.1"
                     /translation="MKKIDAIIKPFKLDDVREALAEVGITGMTVTEVKGFGRQKGHTE
                     LYRGAEYMVDFLPKVKIEIVVPDDIVDTCVDTIIRTAQTGKIGDGKIFVFDVARVIRI
                     RTGEEDDAAI"
     gene            complement(34323..35657)
                     /gene="glrR"
                     /locus_tag="OO357_001369"
     CDS             complement(34323..35657)
                     /gene="glrR"
                     /locus_tag="OO357_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator GlrR"
                     /protein_id="HCR5886371.1"
                     /translation="MSHKPAHLLLVDDDPGLLKLLGLRLTSEGYSVVTAESGAEGLRV
                     LNREKVDLVISDLRMDEMDGMQLFAEIQKVQPGMPVIILTAHGSIPDAVAATQQGVFS
                     FLTKPVDKDALYQAIDDALEQSAPATDERWREAIVTRSPLMLRLLEQARLVAQSDVSV
                     LINGQSGTGKEIFAQAIHNASPRNSKPFIAINCGALPEQLLESELFGHARGAFTGAVS
                     NREGLFQAAEGGTLFLDEIGDMPAPLQVKLLRVLQERKVRPLGSNRDIDINVRIISAT
                     HRDLSKAMARGEFREDLYYRLNVVSLKIPALAERTEDIPLLANHLLRQAAERHKPFVR
                     AFSTDAMKRLMTASWPGNVRQLVNVIEQCVALTSSPVISDALVEQALEGENTALPTFV
                     EARNHFELNYLRKLLQITKGNVTHAARMAGRNRTEFYKLLSRHELDANDFKE"
     gene            complement(35647..36360)
                     /gene="qseG"
                     /locus_tag="OO357_001370"
     CDS             complement(35647..36360)
                     /gene="qseG"
                     /locus_tag="OO357_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system QseEF-associated
                     lipoprotein QseG"
                     /protein_id="HCR5886372.1"
                     /translation="MRHIFQRLLPRRLWLAGLPCLALLGCVQNHNKPAIDTPAEEKIP
                     VYQLADYLSTECSDIWALQGKSTETNPLYWLRAMDCADRLMPAQSRQQARQYDDGSWQ
                     NTFKQGILLADAKITPYERRQLVARIEALSTEIPAQVRPLYQLWRDGQALQLQLAEER
                     QRYSKLQQSSDSELDTLRQQHHVLQQQLELTTRKLENLTDIERQLSTRKPAGNFSPDT
                     PHESEKPAPSTHEVTPDEP"
     gene            complement(36525..37952)
                     /gene="qseE"
                     /locus_tag="OO357_001371"
     CDS             complement(36525..37952)
                     /gene="qseE"
                     /locus_tag="OO357_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two component system sensor histidine kinase
                     QseE/GlrK"
                     /protein_id="HCR5886373.1"
                     /translation="MKRWPVFPRSLRQLVMLAFLLILLPLLVLAWQAWQSLNALSDQA
                     ALVNRTTLIDARRSEAMTNAALEMERSYRQYCVLDDPTLAKVYQSQRKRYSEMLDAHA
                     GVLPDDKLYQALRQDLNNLAQLQCNNSGPDAAAAARLEAFASANTEMVQATRTVVFSR
                     GQQLQREIAERGQYFGWQSLVLFLVSLVMVLLFTRMIIGPVKNIERMINRLGEGRSLG
                     NSVSFCGPSELRSVGQRILWLSERLSWLESQRHQFLRHLSHELKTPLASMREGTELLA
                     DQVVGPLTPEQKEVVSILDSSSCNLQKLIEQLLDYNRKQADSAVELENVELAPLVETV
                     VSAHSLPARAKMMHTDVDLKATACLAEPMLLMSVLDNLYSNAVHYGAESGNICLRSSL
                     HGARVYIDVINTGTPIPQEERAMIFEPFFQGSHQRKGAVKGSGLGLSIARDCIRRMQG
                     ELYLVDESGQDVCFRIELPSSKNTK"
     gene            complement(38510..42397)
                     /gene="purL"
                     /locus_tag="OO357_001372"
                     /gene_synonym="purI"
     CDS             complement(38510..42397)
                     /gene="purL"
                     /locus_tag="OO357_001372"
                     /gene_synonym="purI"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708394.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase"
                     /protein_id="HCR5886374.1"
                     /translation="MMEILRGSPALSAFRINKLLARFQAARLPVHNIYAEYVHFADLN
                     APLNDDEHAQLERLLKYGPALASHAPQGKLLLVTPRPGTISPWSSKATDIAHNCGLQQ
                     VNRLERGVAYYIEAGTLTNEQWQQVTAELHDRMMETVFFDLDDAEQLFAHHQPTPVTS
                     VDLLGQGRQALIDANLRLGLALAEDEIDYLQDAFTKLGRNPNDIELYMFAQANSEHCR
                     HKIFNADWIIDGEQQPKSLFRMIKNTFETTPDHVLSAYKDNAAVMEGSEVGRYFADHE
                     TGRYNFHQEPAHILMKVETHNHPTAISPWPGAATGSGGEIRDEGATGRGAKPKAGLVG
                     FSVSNLRIPGFEQPWEEDFGKPERIVTALDIMTEGPLGGAAFNNEFGRPALNGYFRTY
                     EEKVNSHNGEELRGYHKPIMLAGGIGNIRADHVQKGEINVGAKLVVLGGPAMNIGLGG
                     GAASSMASGQSDADLDFASVQRDNPEMERRCQEVIDRCWQLGDANPILFIHDVGAGGL
                     SNAMPELVSDGGRGGKFELRDILSDEPGMSPLEIWCNESQERYVLAVAADQLPLFDEL
                     CKRERAPYAVIGEATEELHLSLHDRHFDNQPIDLPLDVLLGKTPKMTRDVQTLKAKGD
                     ALVREGITIADAVKRVLHLPTVAEKTFLVTIGDRSVTGMVARDQMVGPWQVPVANCAV
                     TTASLDSYYGEAMAIGERAPVALLDFAASARLAVGEALTNIAATQIGDIKRIKLSANW
                     MAAAGHPGEDAGLYEAVKAVGEELCPALGLTIPVGKDSMSMKTRWQEGNEEREMTSPL
                     SLVISAFARVEDVRHTITPQLCTEDNALLLIDLGKGNNALGATALAQVYRQLGDKPAD
                     VRDVVQLKGFYDAIQALVAQRKLLAYHDRSDGGLLVTLAEMAFAGHCGIDADIASLGD
                     DRLAALFNEELGAVIQVRAADREAVESVLAQHGLADCVHYVGQAVSGDRFVITANGQT
                     VFSESRTTLRVWWAETTWQMQRLRDNPECADQEHQAKSNDADPGLNVKLSFDINEDVA
                     APYIATGARPKVAVLREQGVNSHVEMAAAFQRGGFDAIDVHMSDLLTGRTGLEDFHAL
                     VACGGFSYGDVLGAGEGWAKSILFNDRVRDEFATFFHRPQTLALGVCNGCQMMSNLRE
                     LIPGSELWPRFVRNTSDRFEARFSLVEVTQSPSLLLQGMVGSQMPIAVSHGEGRVEVR
                     DAAHLAALESKGLVALRYVDNFGKVTETYPANPNGSPNGITAVTTESGRVTIMMPHPE
                     RVFRTVSNSWHPENWGEDGPWMRIFRNARKQLG"
     gene            42656..44212
                     /gene="mltF"
                     /locus_tag="OO357_001373"
     CDS             42656..44212
                     /gene="mltF"
                     /locus_tag="OO357_001373"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltF"
                     /protein_id="HCR5886375.1"
                     /translation="MKKLKINYLFIGILALLLAVALWPSIPWFGKADNRIAAIQARGE
                     LRVSTIHTPLTYNEINGKPFGLDYELAKQFADYLGVKLKVTVRQNISQLFDDLDNGNA
                     DLLAAGLVYNSERVKNYQPGPTYYSVSQQLVYKVGQYRPRTLGNLTAEQLTVAPGHVV
                     VNDLQTLKETKFPELSWKVDDKKGSAELMEDVIEGKLDYTIADSVAISLFQRVHPELA
                     VALDITDEQPVTWFSPLDGDNTLSAALLDFFNEMNEDGTLARIEEKYLGHGDDFDYVD
                     TRTFLRAVDAVLPQLKPLFEKYAEEIDWRLLAAIAYQESHWDAQATSPTGVRGMMMLT
                     KNTAQSLGITDRTDAEQSISGGVRYLQDMMSKVPESVPENERIWFALAAYNMGYAHML
                     DARALTAKTKGNPDSWADVKQRLPLLSQKPYYSKLTYGYARGHEAYAYVENIRKYQIS
                     LVGYLQEKEKQATEAAMQLAQDYPAVSPTELGKEKFPFLSFLSQSSSNYLTHSPSLLF
                     SRKGSEEKQN"
     gene            complement(44209..44745)
                     /gene="tadA"
                     /locus_tag="OO357_001374"
     CDS             complement(44209..44745)
                     /gene="tadA"
                     /locus_tag="OO357_001374"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HCR5886376.1"
                     /translation="MRRAFITGVFFLSEVEFSHEYWMRHALTLAKRAWDEREVPVGAV
                     LVHNNRVIGEGWNRPIGRHDPTAHAEIMALRQGGLVMQNYRLIDATLYVTLEPCVMCA
                     GAMIHSRIGRVVFGARDAKTGAAGSLMDVLHHPGMNHRVEITEGILADECAALLSDFF
                     RMRRQEIKAQKKAQSSTD"
     gene            complement(44770..45405)
                     /gene="yfhb"
                     /locus_tag="OO357_001375"
     CDS             complement(44770..45405)
                     /gene="yfhb"
                     /locus_tag="OO357_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417055.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase C"
                     /protein_id="HCR5886377.1"
                     /translation="MATHERRVVFFDLDGTLHQQDMFGSFLRYLLRRQPLNALLVLPL
                     LPIIAIALLIKGRAARWPMSLLLWGCTFGHSEARLQTLQADFVRWFRDNVTAFPLVQE
                     RLTTYLLSSDADIWLITGSPQPLVEAVYFDTPWLPRVNLIASQIQRGYGGWVLTMRCL
                     GHEKVAQLERKIGTPLRLYSGYSDSNQDNPLLYFCQHRWRVTPRGELQQLE"
     gene            45614..46462
                     /locus_tag="OO357_001376"
     CDS             45614..46462
                     /locus_tag="OO357_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HCR5886378.1"
                     /translation="MNGLLRIRQRYQGLAQSDKKLADYLLLQPDTARHLSSQQLANEA
                     GVSQSSVVKFAQKLGYKGFPALKLALSEALASQPESPSVPIHNQIRGDDPLRLVGEKL
                     IKENTAAMYATLNVNSEEKLHECVTMLRSARRIILTGIGASGLVAQNFAWKLMKIGFN
                     AAALRDMHALLATVQASSPDDLLLAISYTGVRRELNLAADEMLRVGGKVLAITGFTPN
                     ALQQRASHCLYTIAEEQATNSASISACHAQGMLTDLLFIALIQQDLELAPERIRHSEA
                     LVKKLV"
     gene            complement(46801..47289)
                     /locus_tag="OO357_001377"
                     /pseudo
     CDS             complement(46801..47289)
                     /locus_tag="OO357_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015364430.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
BASE COUNT        11272 a        12958 c        11895 g        11164 t
ORIGIN      
        1 cttgcaggct ggcagcgcga tgacctgcta ttagaagaag gtgcagtaga gctgccggaa
       61 ggagagttta acgccgcgtt taatcctgaa gcagtggtga aagtaaccga tgtgttattg
      121 gcaagccatg aaaatacggc gcaaattatt gatgcccgcc cggctgcacg ttttaacgca
      181 gaagttgatg aacctcgccc aggtttacgt cgcggacata ttcccggtgc attgaacgtt
      241 ccgtggacgg aactggtgcg cgaaggcgaa ctaaaaacga ccgatgaact ggatgcgata
      301 ttttttggtc gcggcgtcag ctatgacaaa ccgattatcg tcagctgcgg ctctggtgta
      361 acggcagccg tggttttgtt agcactcgcg acgctggatg tgccaaacgt gaaactgtac
      421 gacggcgcat ggagtgaatg gggcgcgcgg gcagatttac cggttgagcc agtgaaataa
      481 gtatattaca ggcaataaaa aaccgccgaa tttggcgttt ttttattgct ggtctggttc
      541 gcggcctttc cagcaggttg acttgtgtta catgagcaac gcaggtgctt cacagcaaaa
      601 caatactcac cagtaactct ctttttgtca agcaaaagag agtaattatt gtttatttag
      661 cgtattatcg acaccggccc tttccgccgt gttcggtaat aaaataacct ggcttattag
      721 tccgaattca gacaaatata aacaaatcct gctcaaaatt aaaaattcta accggtaaaa
      781 gatattactt aaacatgtaa atttactttc ctttaaaaaa caaaaaaccg ccaaaatcag
      841 gcggttattt gttgctggtc cggttcgcgg cctttccagc aggttgtatt accgtagtaa
      901 tgcaagcgcg tctcagcgga gacaatactc gccagtaact ctctttttgt caagcaaaag
      961 agagttatta ttgttctgtt agtgtattat ccactgcggc cctttccgcc gtctcgcaag
     1021 cgggcgctgg cttaaggaaa ggatgttccg cgaccgttag agcaagcgcg tctcagcgct
     1081 tgctatcgcg gcaatatcgc cagtggtgct gtcgtgatgc ggtcttcgca tggaccgcac
     1141 aatgaagata cggtgcttct gtatcgtact tattgtttct ggtgcgctgt tttcccaagt
     1201 aaataataac cggagtctct ccggcgacaa tttactggtg gttaacaacc ttcagagcag
     1261 caagtaagcc cgaatgccgc cctttagggc ggcatatttt agattatccg gttttgttta
     1321 aagtcacgca aaaaaccacc ccagcgacgt tcatagaatg gcgcaatatg ttcggtaata
     1381 aagtggctaa ttcctttttc cccttttttc acctgacaaa tatcgattgg ttcatcgcca
     1441 ggtaatgtat cggtcgctac acttcccgtc gcctgaataa tttcttcgat atcaccatcg
     1501 gcttcaatgc caataagtaa attaggctgt gcctcttcgt tctctttaat tgaacaaata
     1561 aaagcacgct tcaccggctt aatggtttta aataaggtgg tgagtgaatc aatcatttgt
     1621 gctggcggct ctgcgacttc cgataatatc agcgattcac cgccttccag gatttcctgg
     1681 ctgctcagcg gatttccctc ttcaccaatc aacaaactga tttcacgcgg cataaattct
     1741 ttaccggtcg gcagtttggc attaaggaag agcgtttcgc caagggtcat ctcaaacaat
     1801 gtgcgaacgg gcattacgac aaatgcctgt tcgtcttcaa ccgcctgttg aagcgcttct
     1861 aacgaggtga aaaaaggaat gacgctggtg ccgtcttctt tttcccagtg ctgtaaatca
     1921 agcgcactat cttcaaccac agcctcgccc tgcgccgccg taccaggcac ccagacggtg
     1981 gattccagta gagtacggaa aaaggccggg cggtgcgccg gttcggttgc tgctttttcc
     2041 agcaggtctt caagttcgtt ttttgtttcg gacataagaa ccacaattca ttcaacgttc
     2101 ggggcgcaaa tgcctgatgc gctacgctta tcaggcctac aagggattcg caatttgttg
     2161 aatttgcaga atttgtaggc cggataaggc gtttacgccg catccggcat attagtttac
     2221 gccgttaaca gattagctat cgtgcgcaca ccaagtcccg tagcgcccgc agaccactgt
     2281 tcaaccggcg ctttacggta agtcgccgag cagtcgatat gcagccagcc ttgctgatag
     2341 ttctcaacaa agtgcgacag gaagcccgcc gccgtgctcg cgcctgccgg atacgccgcg
     2401 cttccggtat tgttcagttc ggcaaagtta gacggcagct ggctgcggtg gaattccgcc
     2461 agcggcagac gccagaatgg ttcgttctct tgtgaggcac tcgccagtaa acgaccggca
     2521 agcgcatcgt caaaactgaa cagcgcgtga taatcattac ccagcgcagt tttcgccgcc
     2581 ccggtgaggg tcgccgcatc aatgatcatt tccggtttct gcgcactggc atcaatcaga
     2641 ccatcggcaa gcaccagacg cccttcggca tcagtgttca tcacttcaac ttttttaccg
     2701 ttgcgatagg tgatgatatc gcccagcttg aacgcattgc cgctaatcag gttatccgca
     2761 cagcagagga acagcttcac gcgcttgttc agtccgcgca taatggcaaa tgccagcgcc
     2821 ccggtaaccg ttgccgcgcc gcccatgtcc gacttcatcg agtccataaa cgcagtctgt
     2881 ttgatgctgt agccgccgga gtcaaaagtg atacctttac ctaccaggca cgcgtacacc
     2941 ggtgcttctt tatcgccagt tgggttgtaa tccagcgcca gcaataccgg agaacgttct
     3001 gaaccgcgcc cgacagtgtg cagccccata taaccttgct cacgcagatc ttcgcctttg
     3061 gtgatccgat aagtcacacg atcgcccgcg acgttgctga tcagatcaac agcacgctgt
     3121 gccagttgcg atggtcccaa ttcttctgcc ggtgcgttga tggtgtcacg cacccagtcg
     3181 atgatcatca ggcggttatc cagttcctgg cgctgggcat cgtccagatc cggccactcc
     3241 actttacgcg tgcctttcgg gcctttataa ccttgccaga atgcccagca gcgatccgca
     3301 tcccagcctt caccgcttaa ctgaacatgc ttgatgccca gaccgtcaat cttgcgggcc
     3361 gcacgctgga tcagccccag atcgtctgcc ccgttcaaat gcagggtaat gccgtcatta
     3421 ttaatgctgt aagttgcttt ttctccccag cgcgcgtcgg caggttgggt agagagggta
     3481 atcttcatcg cttctgtcat tttagttatc cttattcgtt taacagcaaa acgggctacc
     3541 cccaggcagc ccgttcattc actcggcttc gtccagccag actaacaaaa tcgcttcgag
     3601 gattttctcg ttggatgcct gcgggtcgtc gtcgaaatct tccagatcgc aaatccactg
     3661 atgcatatcg gtgaatcgaa ccgttttcgg atcaagatcg ggatacgcat cgtacagtgc
     3721 ttcgccaatt tcgcggctat cggtccactt aagtcccata ctaacctctg ttaatgctca
     3781 cgcgcatggt tgatagtgta acgcgggatt tcgaccacta aatcttcgtc ggtgacgcgc
     3841 gcctggcagc ttaaacggct ttccggctcc agtccccagg ctttgtccag catgtcgtct
     3901 tcctgctctg agctttccgg cagtgagtca aaaccttcac gaacgatgca gtggcaggtg
     3961 gtgcaagcac aggatttttc acaggcgtgt tcaatctcaa taccgttacg cagcgctgcg
     4021 tcgagaatgg tttcaccgct attagcttcc agaacagcgc catcagggca gaggtcctga
     4081 tgaggcaaaa taacaatctt tggcatatta aacctcgtcc acggaatggc ctttcagcgc
     4141 acgacgaacc gactggtcca tgcggcgagc ggcgaaatcc tgggtttgtt tgtctacgtt
     4201 tttaatcgct tgttcgatgg cgtcaacatc atcgccctgc gccacttcac tcaggtgagc
     4261 ggcagcattg tcaatgacct gacgctctgc ggcgcttaac agcgcggcat cagcagccag
     4321 cgcgccgtgc agactttcca gcacacgcgc cgcttctact ttttgttctg ccagcatacg
     4381 ggcttttacg tcctgctcgg catagctcat tgagtctttg atcatcgaag cgatttcgct
     4441 atcggtcaga ccgtaagacg gtttgacctg aatagacgcc tcaacgccgg tggatttctc
     4501 catcgccgtc acgctcaaaa gaccgtcggc atcgacctgg aacgtcacgc gaatatgcgc
     4561 accgccagcc ggtagcgccg gaataccacg cagcgcaaaa cgcgccagtg agcggcagtc
     4621 ctgcaccagt tcgcgctcac cctgcattac atggatagac atcgccgtct gaccatcttt
     4681 aaaggtggtg aaatcctgag cgcgggccac cggaatagtg gtattacgcg gaatcacttt
     4741 ctccaccagg ccgcccatcg tttcgaggcc cagcgacagt gggatcacat caagcagcag
     4801 catttcgctg tctggcttgt tacccaccag aatatccgcc tgaatcgccg cgccaatagc
     4861 gacgacttta tccgggtcga tggaagtcag cggtggacga ccgaaaaatt cgcctacccg
     4921 ttcacgcacc agcggcacgc gagtagaacc gcccaccatc accacttcca gcacttcgtc
     4981 agcttctaca cccgcgtctt tcagcgcgcg acgacaagcc agtaaggttc gttttaccag
     5041 tggcgcgatc agttcattga attgttcacg gctgatttcg ccctgccagc ccgcaacgtt
     5101 aacggtcacg gagtccgcat cgctcagcgc gattttggct gcaatggtgg catccagcag
     5161 ttcacgctga acgcggttat cgctacgatc aggaatgtcc gcctgctcgc gaatgtaatc
     5221 cgccagcaga tggtcgaaat catcgccgcc gagcgcggaa tcaccgccgg ttgccagcac
     5281 ttcaaacacg ccgcgactta agcgcagaat ggaaatatca aacgtcccgc caccgaggtc
     5341 ataaacagcg atcacgcctt cctgaccgga atccagcccg taggcgatag ccgcagcagt
     5401 cggttcgtta agtaagcgca gaacatgcaa ccccgccaga cgcgccgcgt ctttggtgcc
     5461 ctgacgctgg gcatcgtcaa agtacgccgg aacggtgata actacaccat ccagctcgcc
     5521 tgccagggct tcagttgccc gcgccgccag tgctttgagg atgtccgcag aaacgcgcac
     5581 cgggttcagt agccccgccg ccgtttcaat catcggcagg ccgttttcgc tggcctggaa
     5641 ttgataaggc agatgcggat agcgttgctg gatatcagcc agcgagcgcc ccatcaggcg
     5701 tttaacagaa ctaatggtgt tggcggtgtc aagcgctgcg ttagtacgcg cgtcataacc
     5761 caccgaatgc ccttgctgtt gatagtgaac aacagatggc agcaggtgac ggccttcatg
     5821 gtcggctaac gtttcggcct gaccgctgcg cactgtcgcc accagcgagt tggttgtgcc
     5881 caggtcaata ccagccgcca gacgacgctg atgcggcgca gcactcaaac caggttcact
     5941 aatttgtaat aaggccatgt ttagcttcca gaaattaaaa atcgagcagt ttttcttcga
     6001 gttgttcggc actgctccgc agtttatcga gaaaacgcag cttacgcacc gtatccgccg
     6061 ccgcgtccca cgcctcgttg tctaactgtt caaccatcaa ctgatggcgg gtatcaaaca
     6121 tctttttcac acgtttgata aagctttcca gccgcgcttc atctttcgcc tgttcgatct
     6181 cgtccagctc ttcgcgcagc tccaactgtt ccatcaggaa cgcggtgtcg cgcacagtat
     6241 gctgctcgct ggcgagatca aaaccgtgta aagaaagcaa atattccgcg cgcattaacg
     6301 gatgacgcag cgtttgccag gcctggttaa tggttgcaga ttgctgtacg gcggcgagtt
     6361 gttccgccag gcttccgctg gcgaatttat caggatgata ctgacgttgt agatcctgaa
     6421 aacgcaggct cagtgcctgg gtatcgagtt gataacgggc aggcaagcca aagagggtga
     6481 agtaatccat aacattctca gggctgcgat ccgccgcgat aacggcgaat caggtaaaac
     6541 aaaccccacg cgcaggcgac cacggtgggg ttatcggtat gcgcatcaaa cgtggaagct
     6601 ttcgccgcaa ccacactcat ctttaacgtt cgggttggtg aatttaaacc cttcattcag
     6661 gccttctttt acgaagtcca gctgcgtacc gtccagaaat tgcaggcttt tgccatcgac
     6721 cacgactttc acgcctttgt cttcaaacac gatgtcttcc ggcgtcggtt cgtcaacaaa
     6781 ttccagtaca taagccatac ctgaacatcc ggaggttctc acgcccagac gcaggccaaa
     6841 ccctttgccg cggttagcca gaaaggtatt tactcgcgct gctgcactgt cgctcagtgt
     6901 aatcgacata accaaacctc aactcttatt ttgcttcacg tttgctttta tagtccgcaa
     6961 tggcggcttt gatcgcgtct tctgccagaa tagaacagtg aattttcacc ggcggcagtt
     7021 caagttcttc agcaatatcg gtgtttttga tcgcctgcgc ttcgtcgaga gacttccctt
     7081 tcacccattc ggtgaccagg gagctggaag cgatagcgga accgcagccg taagttttaa
     7141 aacgcgcgtc ttcaatgata ccttcatcgt tgactttaat ctgcaacttc attacgtcgc
     7201 cacaggccgg tgcacccacc atgccgctgc cgacgttctc gtcgttgtta tcaaaggaac
     7261 ccacgttacg cggattctcg taatggtcga taactttttc gctgtaagcc attataaatt
     7321 ctcctgattc cgataccgat taatgatgag cccattcgat gctgttcaga tccacgccct
     7381 gcttgtacat ttcccacagc ggagaaaggt cacgcagacg accgatggat ttacgaacta
     7441 actcgatggt gtagtcgatc tcttcttcag tagtaaaacg acctaaagag aaacggatag
     7501 agctatgtgc cagctcgtcg ttcagcccca gcgcgcgcag cacgtaggac ggttcgaggc
     7561 ttgctgacgt acaggcggaa cctgaagaaa ctgcgaggtc tttcagcgcc ataatcagcg
     7621 actcaccttc aacgtagttg aagctgacgt tgagaatgtt cggcgcaccg tgttccaggt
     7681 caccgttcag gtaaacttct tcgatatctt tgatgccgtt ccacagacgg ttacgcaggc
     7741 cgcgcagacg ttccatctcg gtcgccatct cttcttttgc gatgcgatag gcttcgccca
     7801 ttccgacgat ctggtgaaca ggcagagtgc cggaacgcat accgcgctcg tgaccgccgc
     7861 cgtgcatttg cgcttcgatg cgtacacgcg gtttacgacg cacatacagc gcaccgatac
     7921 ctttcgggcc atagattttg tgaccggaga aagacatcag gtcaactttc aactggctca
     7981 ggtcgattgg cagtttgccc acgctctggg ttgcatcaac gtgatagata atgccacgag
     8041 cacggcacat ttcgccgata gccgcgatat cctgcaccac gccgatttcg ttatttacgt
     8101 gcatgatgga aacaaggatg gtgtcgtcac gcatcgccgc ttcgagttct ttcaggtcga
     8161 taataccgtt acgctgcggt gccaggtagg tgacttcgaa accttcgcgc tccagctgac
     8221 ggcaagtatc cagtaccgct ttgtgttcgg ttttgctggt gatgatgtgc ttgccttttt
     8281 tctgataaaa gttggctgca cctttgatcg ccaggttgtc agattcggtt gcaccagagg
     8341 taaagacgat ttcacgcgga tcagcgccga ccagatcggc aatctgatta cgggcgatat
     8401 ctaccgcttc ttcagcctgc cagccgaaac ggtgagaacg agaggccggg ttaccaaagg
     8461 ttccgtccat cgtcatgaac tgcatcattt tctcggcaac acgcgggtcc accggcgtgg
     8521 ttgcggagta gtcgagataa atcggtaatt tcattgctct ataaactccg tacatcactc
     8581 aatgcaagga atcaggctac cggctggatg tacgaccgtg tttacggagt atttagcact
     8641 ccggcctgat tctgaattct ttttattaag cgcgtaactt aacgtcgatc gcgtcttgtg
     8701 tgcgggtgcg tggcgcgtcg tgagtatgct gacgaccaga cacatccagc acttcctggt
     8761 tattaaccag ttcgcctaaa gtaatgttgt tgagaaaacc ggtgagacgg tcgctcaaat
     8821 cacgccacag cgcgtgggtc aggcatttat cgccgccctg gcagccgcct ttaccctgac
     8881 aacgggtggc atctacagat tcgtcaacgg cgctaatcac ttcgccaacg gcgatgctgc
     8941 tggcatcttt gcctaacaga taaccaccgc ctggtccacg tacgctggaa accagaccat
     9001 ttttacgcag acgggaaaac agttgttcca gataagaaag ggaaattccc tgacgttcgg
     9061 aaatatcagc caacggtacc gggcccgctt cagagttgag cgcaacgtca agcattgcgg
     9121 tcacggcata gcgcccttta gatgtcagtc tcatgtctta cttcacctca aactcgcccc
     9181 tgcccggggt tttttattgt aaaagtgggg gtattgcata gcagggtcaa gtctgacatt
     9241 cccgagtaaa ttggtcaact atttacttga ctgatttagt cgggtattta accttcagtg
     9301 ccattttttt atcgtggcgt ttgtacctgt tgtcggatgc ggtgtgaaca ccttatccga
     9361 cctacgattc tgtacattgt aggcctgata agacgcgtta agcgtcgcat caggcaacca
     9421 ctgttattcg gccttgttac ctttattctg ctgctcaata gaagccagaa tcccgcgcag
     9481 gatattcaat tcctggcttt ccgggcgcgc acgggtaaac agacggcgca atttattcat
     9541 cacctgcccc ggatggtttt cacggataaa accggttgcc agcagcgttt gttccagatg
     9601 accataaaaa cgctccagat catcgaccag cggatacggc gtctcttcat gctcgacctg
     9661 ctcgccattt tcctgagttg ccagccaggc catacgcact tcataggcaa taacctgaac
     9721 cgccattgcc aggttcagcg aactgtattc cgggtttgcc gcaatcgcga catgataatg
     9781 gcatttctgc aactcttcat tggtcaagcc gacgcgctca cgaccaaaca ccagcgccac
     9841 cggggtattt gccgcttcag cgacgctttt cagaccgcat tcgcgcgggt cgagcatcgg
     9901 ccacggcagc gtgcgggagc gtgcgctggt gcccaccacc aggctacaac cggctaacgc
     9961 ttcgtcgagc gtatcgacga tgtgagcatt accaatcaca tcgctggccc ctgcagccag
    10021 ggcaatcgcc tgggagtcgg gtttcaccag tggattaacc agccacagat tggttaatcc
    10081 cattgttttc atggcacggg caacagaacc catattgccg gtgtgtgacg tctccaccag
    10141 cacaattcga atattttgca gcattgtctt tcttcatcta aagattattc acgcatctta
    10201 tcataaaacg aagacagatg ccgatctcgc tgctatactc tgcgccgttt tcccgttctt
    10261 taacatccag tgagagagac cgatgcatcc gatgctgaac atcgccgtgc gcgcagcgcg
    10321 caaggcgggt aatttaattg ccaaaaacta tgaaaccccg gacgctgtag aagcgagcca
    10381 gaaaggcagt aacgatttcg tgaccaacgt agataaagct gccgaagcgg tgattatcga
    10441 cacgattcgt aaatcttacc cacagcacac catcatcacc gaagaaagcg gtgaacttga
    10501 aggtactgat caggatgttc aatgggttat cgatccactg gatggtacta ccaactttat
    10561 caaacgtctg ccgcacttcg cggtatctat cgccgttcgt atcaaaggcc gcaccgaagt
    10621 tgctgtggta tacgatccta tgcgtaacga actgttcacc gccactcgcg gtcaaggcgc
    10681 acagctgaac ggctaccgtc tgcgcggtag caccgctcgc gatctcgacg gtaccattct
    10741 ggcgaccggc ttcccgttca aagcaaaaca gtacgccact acctacatca acatcgtcgg
    10801 caagctgttc aacgaatgtg cagacttccg tcgtaccggt tctgcggcgc tggatctggc
    10861 ttacgtcgct gcgggtcgtg ttgacggttt ctttgaaatc ggtctgcgtc cgtgggattt
    10921 cgcggcaggc gagctgctgg ttcgtgaagc gggcggcatc gtcagcgact tcaccggtgg
    10981 tcataactat atgctgaccg gtaacatcgt tgctggtaac ccgcgtgttg tgaaagccat
    11041 actggcgaac atgcgtgacg agttaagcga cgctctgaag cattaatgac tcaggcgggt
    11101 gatatcactc acccgccctc gcctttcagg cgctattccg aaatacttcc tcaccgcttt
    11161 actttctttc gtcactctcc caccatcttt ccccgattaa tggatgataa agaagtcgca
    11221 aggaaaggtt atggcactgc cagtgaacaa acgcgttccc aaaattctgt ttattctctt
    11281 tgttgttgcc ttctgcgttt atttagtgcc gcgcgttgcc atcaacttct tctattatcc
    11341 tgacgataaa atttacggtc ccgacccctg gtcggcggaa tccgtcgaat ttacggctaa
    11401 ggacggtact cgtctgcaag gctggtttat tccttcttcg acgggccctg ctgacaacgc
    11461 catcgcaacc atcattcatg ctcacggcaa tgccggaaat atgtccgccc actggccgct
    11521 ggtcagttgg ttacctgagc gtaatttcaa cgtttttatg tttgattatc gcgggtttgg
    11581 taaatcaaaa ggcactccgt cccaggccgg attgctggac gatacgcaaa gcgccatcaa
    11641 tgtggtgcgc catcgcagtg atgtaaaccc acaacagctg gtgctgttcg ggcagagtat
    11701 tggcggggcg aatattcttg ctgttattgg tcagggtgat cgtgaaggca tacgtgcggt
    11761 gatcctcgac tccacatttg cctcttatgc aaccatcgcc aaccagatga tccccggcag
    11821 tggctactta cttgatgaga gttacagcgg cgaaaattac atcgccagcg tcagcccgat
    11881 cccgctttta ctcattcacg gtaaagccga tcacgttatc ccatggcagc acagcgaaaa
    11941 gttgtatagc ctggcaaaag agccaaaacg gttgatccta atcccggatg gcgaacacat
    12001 tgatgctttt tccgatcgtc acggcaatgt ttatcgcgaa cagatggtgg actttatcct
    12061 tagcgcgttg aatccgcaga actaacccat gatcgctagc acgataatca ttcacaaaac
    12121 caccttaaga catgctaatc cactggtcag aacagtttaa aatgagaaaa attctgtgac
    12181 gcttgccaac atttctgatg attagcattc ccttcgccat ttccctgagc aaactttagc
    12241 tattcttatc aattatgctt atgggagatc tacagatgat gcctacgctt gctccaccat
    12301 ctgtcctttc ggctccccag cgccgctgcc agatcttgct gacgctcttt cagccggggt
    12361 tgaccgccac catggcaacc ttcagcgagc ttaatggtgt ggatgatgat attgccagtc
    12421 ttgatatcag cgaaacagga cgggagatcc tgcgctatca tcaactcaca ctgacgactg
    12481 gttatgacgg tagctaccgg gttgaaggta cagtgcttaa ccaacgtttg tgtttatttc
    12541 actggctacg acgtggtttc cgtctgtgtc cgtcatttat taccagccaa ttcacccccg
    12601 ccctgaagag tgaactgaag cggcgcggaa ttgcgcgtaa cttttacgac gataccaatc
    12661 tacaagcgtt agtgaatctc tgctcccgac ggctgcaaaa acgctttgaa tcgcgcgata
    12721 ttcatttcct gtgtctgtat ctgcaatatt gtttgctgca gcaccacgct ggaattacgc
    12781 ctcagtttaa tccgctccaa cgtcgctggg cggaatcctg ccttgaattt caggtagcgc
    12841 aggaaattgg acgccactgg cagcgtcggg cgctccagca tgtaccgcct gatgagccac
    12901 tgtttatggc attacttttt tccatgttgc gggttcccga tccactgcgg gatgcgcatc
    12961 agcgggacag acaattgcgt cagtctatca aacgtctggt aaaccatttt cgtgagctgg
    13021 gaaatgttcg tttttatgat gaacaggggt tatgtgatca gctttatatc cacctcgccc
    13081 aggcgttaaa tcgcagtttg tttgccatcg gtattgataa taccctgccg gaagagttcg
    13141 ccagactgta tccacgcctg gtacgcacca cccgcgcggc gctggccgga tttgaaagtg
    13201 aatacggcgt ccatctttct gatgaggaaa gtggtctggt cgcggtgatt ttcggtgcct
    13261 ggctaatgca ggaaaacgat ctgcatgaaa aacagattat tctactgacc gggaatgata
    13321 gcgagcgaga agcgcagatt gagcagcagt tacgcgaact aacgttactg ccgctcaaca
    13381 ttaagcatat gtcggtaaag gcatttttgc agacaggcgc tccgcgtggc gcggcactga
    13441 ttattgcgcc ttataccatg ccgttaccgc tcttttcacc accgctgatc tatacggacc
    13501 tgacgctgac aacacatcaa caggagcaga tccgcaaaat gctcgaatca gcatgaggga
    13561 acaactttcg ggcgcaaaaa cattgccgga agcgccacca gcgccattac ccagaacacg
    13621 ccgtggccca gatattgata caggaaaccg gcgaaaacgg tcatgatagc gatactgccg
    13681 cccatcgcga cggcagagta aaccgcctgt aaacggatga cttcgctaac ctggcgagca
    13741 gcaatataac gcatggcggc caggtggcag accgtgaagg tgccgcaatg cagaatttgc
    13801 accactatca accacggcaa cgccgtagtt gctcccataa tgccccagcg cactacgccg
    13861 caaatcgccg agatcaacag catatcgcga gcactacaac ggcggaaaag tttattactc
    13921 agcgcaaaga taatgacttc cgccaccacg cccagcgacc acaaataccc caccgccgag
    13981 gccgagtagc cagctgcctg ccagtaaatg gcgctaaaac cgtaataggc cgcatgtgcc
    14041 ccctgcaata aacaaacgca ggccagaaag cgccagttct ggcgaaccag cgccaaccac
    14101 gcagaccaac cggtgctctc ctgctggcgg cttgcccctt gtggctgaat cgtcggacgg
    14161 atgagaaagc cgagcagcat ggatgccacg cccaacgtca acagcacgag gatcacccga
    14221 taatcaaaca tagtgaccag tttgcccgtc agcgccgagc caatgacaaa cgccaccgag
    14281 ccccacagtc gcactttgcc gtaatcaagc gggaactgct tttgccacgt attcgccagt
    14341 gcatcggtca acggtaccag cggtgagaaa aagaggttaa agccaatcat caccagcatc
    14401 agccacgcta cgtgcgcccc cgcccagaag gcgacagcaa agagaagtgg cagcagtgcc
    14461 agcacgcgca aggcggaaat caggcgggaa ggatcgctga cgcggggcgc gatgagcaaa
    14521 ctcccgagaa aacgggcaac cagacctgcc cccaataaca ggccgatggt ttctggcgtt
    14581 aaaccaatcc ctttaagcca gacgctccag aaaggtaaaa aaatgccgta actaaaaaag
    14641 tatgtgaaat agccgagcgc caaccagcgc gtggattgca aaaccatgag tccctcccgt
    14701 caggaggcgt tagtctgggg ttaatcgaat gttggtgcaa gttgaaaaat aagaatattc
    14761 gagcctgctt cccgatgtct gtaacgccag ttcacaagca ctaaaaaact gcgatccgcg
    14821 acgcatgttg caaaaataat gcgtaattat gccgttacgc ttgccaaacg ttcctgcaca
    14881 atggcgatga aatcgcgtaa tgccagcttc atctcccctt tcttccacgc catcagtaaa
    14941 gcaatggaag gcacatttcc ggcaatggga cgaaaaacaa cctgcccggt attaaaatta
    15001 ttcatataac cgggtatcaa agtgacgccc agccccatgc ccaccagatt catggtcacc
    15061 agaatattcg ttgccacctg gacgatattt ggctggctgt tttcttgcgc aaaccacgct
    15121 ttaacgatcg gcgcaagcga accggaatac gccggatcgg tactgacgaa attcacgcca
    15181 tccagttgcg cggcggtgat ctctttttca tgtgctaaag ggtgatcaac cggtaacacc
    15241 accactaatg gttcgtcaaa aagctccaga taatcaatct ccgggctata aacaggatgg
    15301 cgcattaagc cgacgtcgag ttcaccacgg cgaatttttt cctcctgttg cgtggtgatt
    15361 aaactcacca gctcaatcaa ggtgtctggc tgtctgagac gaaacatcgg taatactttt
    15421 ggcaataaat tcacttccgc cgatggcaca aagccaatgg ttaattgtct gtcttcctga
    15481 acaattttcc gcgcccgtaa tttggcattt tccgcttgtt cgaggattgc cagcgcgtcc
    15541 tggagaaaac attctcccgc cgccgtcaac gcgactttgc gcttatccct caccagtaac
    15601 ggaacaccga cacagttttc aagatcgcgg atctggctgc ttaacgaagg ctgtgaggta
    15661 tgcagttttt ccgccgcacg ggtaaagttc agtgcctgcg ccactgcgac gaaatagcgt
    15721 aaatgccgta gttccatcac cttccccttg ttatcgaaaa aacgtctcag ctggtagaaa
    15781 ttaactattt cacatattag caaccaacca gcaacatcct tatggcacaa aaatagaagg
    15841 tcaatacatc ttatctttca ggattaaaaa atatgaccac accctcagat ttgaacattt
    15901 accaactgat tgacacccaa aacggtcggg tcactccgcg tatttatacc gacccggaca
    15961 tttaccaact ggagcttgag cgtattttcg gtcgttgctg gctatttctc gcccacgaaa
    16021 gccagatccc aaaacccggt gatttcttta acacctacat gggagaagat gcagttgtcg
    16081 tggtgcgcca gaaagacggc agcatcaagg cgtttctcaa ccaatgccgc caccgggcca
    16141 tgcgtgtgag ttatgcagat tgtggcaaca gtcgcgcctt tacctgcccg tatcacggct
    16201 ggtcttatgg cattaacggc gagttgatcg atgtaccgct ggaacctcgc gcctacccac
    16261 aagggttgtg taaatcccac tgggggctaa acgaagttcc ttgtgtggag agttataaag
    16321 gactgatttt tggcaactgg gataccagcg caccgggcct gcatgattac ctgggtgaca
    16381 ttgcctggta tctggatggc atgctggatc gtcgcgaagg cggcaccgaa attgtcggcg
    16441 gcgtacaaaa gtgggtgatc aactgtaact ggaaattccc ggcagagcag ttcgccagtg
    16501 accagtatca tgctctgttc agccatgctt ctgccgttca ggtattaggg gcgaaagatg
    16561 atggcagcga taagcgcctc ggtgatggac aaaccgcccg cccggtgtgg gaaaccgcca
    16621 aagatgcgct gcaatttggt caggacggtc acggtagcgg tttcttcttt actgaaaaac
    16681 cggatgctaa tgtctgggtc gatggcgcag tttcaagcta ttaccgcgaa acctatgccg
    16741 aagcagaaca acgtttaggt gaagttcgcg ccctgcgcct ggcgggtcat aacaatatat
    16801 tccccacgct ttcatggcta aacggcactg ccacactccg cgtctggcat ccgcgcggcc
    16861 ctgatcaagt tgaagtgtgg gcgttctgta ttactgacaa agccgcctcc gatgaagtta
    16921 aagccgcttt tgaaaacagc gccactcgtg cttttggtcc tgctggtttt ctcgagcagg
    16981 atgactcgga gaactggtgt gaaatccaga aattgcttaa aggccaccgc gcccgcaaca
    17041 gcaaactgtg tctggaaatg gggcttggtc aggaaaagcg ccgcgacgac ggcattcctg
    17101 gcattactaa ctatatcttt tcagaaactg ccgctcgtgg aatgtaccaa cgctgggccg
    17161 atcttctgag tagcgaaagc tggcaggaag tgctcgataa aaccgccgct taccagcagg
    17221 aggtgatgaa atgagtgcgc aagtttcact ggagttacat caccgcatta gccagtttct
    17281 ctttcacgaa gccagtctgc tggatgactg gaaatttcgt gactggttgg cacagctcga
    17341 cgaagagatt cgttacacca tgcgcaccac ggttaacgcg caaacacgcg accgtcgcaa
    17401 aggcgtccag ccgccgacga cctggatttt caatgacacc aaagaccagc tggagcggcg
    17461 aatcgcccgt ctggaaacgg gcatggcctg ggcagaagag ccgccgtcac gcactcgtca
    17521 cttaatcagc aactgccagg taagcgaaac cgacatccca aacgtatttg ccgtgagggt
    17581 gaattatcta ctttatcggg cacaaaaaga gcgcgatgaa acattctatg ttggaacgcg
    17641 tttcgacaaa gttcgccgtc tggaagatga caactggcgc ttgctggaac gggatatcgt
    17701 cctggatcaa gcggtgatca cttcccataa cctgagtgta ctgttctgat gaatcgaatt
    17761 tatgcgtgtc ccgttgcgga tgtgccggag ggtgaggctc tccggataga tacctcgccc
    17821 gtcatcgcct tgttcaacgt gggcggcgag ttttatgcca ttaacgatcg ttgcagccat
    17881 ggtaatgcgt cgatgtcaga agggtatctg gaagatgacg ccacggtgga gtgcccgcta
    17941 cacgccgcca gtttttgcct gaaaacgggg aaagcgttat gcctgcccgc caccgatccg
    18001 ctcaccactt atccagtaca cgttgaaggt ggtgacattt tcatcgactt accggaggcg
    18061 cagccatgag cgatctgcat aacgagtcca tttttattac cggcggcgga tcgggattag
    18121 ggctggcgct ggtcgagcga tttatcgaag aaggcgcgca agttgccacg ctggaactgt
    18181 cggcggcaaa agtcgccagt ctgcgtcagc gatttggcga acatattctg gcggtggaag
    18241 gtaacgtgac ctgttatgcc gattatcaac gcgcggtcga tcagatcctg actcgttccg
    18301 gcaagctgga ttgttttatc ggcaatgcag gcatctggga tcacaatgcc tcactggtta
    18361 atactcccgc agagacgctc gaaaccggct tccacgagct gtttaacgtc aacgtactcg
    18421 gttacctgct gggcgcaaaa gcctgcgctc cggcgttaat cgccagtgaa ggcagcatga
    18481 ttttcacact gtcaaatgcc gcctggtatc ctggcggcgg tggcccgctg tacaccacca
    18541 gtaaacatgc cgcaaccgga cttattcgcc aactggctta tgaactggca ccgaaagtgc
    18601 gggtgaatgg cgtcggcccg tgtggtatgg ccagcgacct gcgcggccca caggcgctcg
    18661 ggcaaagtga aacctcgata atgcagtctc tgacgccgga gaaaattgcc gccattttac
    18721 cgctgcaatt tttcccgcaa ccggcggatt ttacggggcc gtatgtgatg ttggcatcgc
    18781 ggcgcaataa tcgcgcatta agcggtgtga tgatcaacgc tgatgcgggt ttagcgattc
    18841 gcggcattcg ccacgtagcg gctgggctgg atctttaagg aagcacgatg aaagaaaaaa
    18901 cgatcattat tgtcggtggc gggcaagcgg cggcaatggc tgcggcctcg ctacgccagc
    18961 aagggttcac cggtgagctg catctgtttt ccgatgagcg acatcttcct tatgaacgac
    19021 ctccactctc gaaatccatg ttgctggaag attccccgca gttacagcag gtgttacccg
    19081 ctaactggtg gcaggaaaac aatgttcatc tgcattccgg tgtaaccatc aaaacgctgg
    19141 gtcgcgacac acgagagtta gtgttaacca acggcgaaag ctggcactgg gatcagcttt
    19201 ttatagcaac cggcgcggca gctcgaccgc tgccgttgct tgatgcactg ggagaacgct
    19261 gctttaccct acaccatgcc ggtgatgccg ccagactgcg agaagttctg cagcccgaac
    19321 ggtcagtcgt gattgtcggt gccggaacta ttggtctgga actggctgcc agcgccacgc
    19381 agcgcagcgc agcgcagcgc agatgtaagg tgacagtgat tgaactggcg gcaaccgtca
    19441 tgggccgtaa tgcaccaccg cccgtgcaac gctatctttt acagcgccat cagcaggctg
    19501 gtgtgcgcat tctgctcaat aatgccattg aacatgtggt cgatggtgaa aaagtagaac
    19561 tgacgctgca aagtggggaa acgcttcagg ctgatgtggt gatttacggt attggtatca
    19621 gcgccaacga gcaactggct cgcgaggcca accttgatac tgccaatggc attgtcattg
    19681 atgaggcttg ccgcacctgc gatcccgcga tctttgccgg tggcgatgtg gcaatcactc
    19741 gtcttgataa tggtgcacta caccgctgcg aaagctggga aaacgccaat aaccaggcgc
    19801 aaattgccgc tgccgcaatg ttagggctac cgctaccgct actgccgccg ccgtggttct
    19861 ggagcgatca gtacagtgat aacttacagt ttattggcga tatgcgtggc gatgactggc
    19921 tttgtcgtgg caacccggaa actcagaagg cgatttggtt taatctgcaa aacggcgtgc
    19981 ttatcggtgc ggtcacgctg aatcagggcc gtgagattcg cccaattcgc aaatggatcc
    20041 agagcggcaa aacgtttgat gcgaaactgc tgatagatga gaacatcgcg cttaaatcac
    20101 tgtaatcagg ataattagcg aatatctcaa tgcctggggc gtggcgaggt gcaagagtgt
    20161 gtattacgtt taaatcacat tatcttgcaa agggattggt tatgaacaca ctacgttatt
    20221 ttgattttgg agctgcccgc cccgttttgt tattaattgc ccgtatcgcc gtggtcttaa
    20281 ttttcattat ttttggtttt cccaaaatga tgggctttga cggtacggtc caatatatgg
    20341 cctcgttggg cgcgccaatg ccgatgctgg cagcgattat tgcggtagtt atggaagtgc
    20401 ccgccgcgat attaatcgtg cttggctttt tcacccgtcc gctggcggtg ctgtttattt
    20461 tctacacgct gggtacggcg gtgattggtc accattactg ggatatgacc ggcgatgcgg
    20521 ttgggccaaa tatgattaat ttctggaaga atgtcagtat cgctggcgcg ttcttgctat
    20581 tggcaattac cggaccgggg gcaatttctc tcgatcggcg ttaggaaaga tgccggatgc
    20641 ggcgtgaacg ccttatccgg cattttataa attacagcca ctctacccgc aacgacatct
    20701 ctgaggttgt tgattcccct ggtgctagcg caatgagatc acccccttcc gggcgatgat
    20761 gctcatccgg cgcatggctc atcggttcca gacagaagaa atcaaacgca tatcctttat
    20821 caaacgcagg gtcggaaaca aagatgaaat aacacggtgc aggtggcgtc gtttccatga
    20881 tgatggcata accttcctgc ggctgctcga tgcgggcctg accattccat ccggcaaaac
    20941 cattgttcac ccactgacgc ggcaacggcg cgagctggtt aaaatccagt tcctgcggta
    21001 gctgctcgca aaactcaccc gccagccact gctcccgttc cagccagtaa ccgctcgcct
    21061 gcgcctgaat ccgcgtttgt ggtgacaacg gaaaataagg atgccagccg gtaccaaatg
    21121 gcagcgtctc tgccccttga ttggtgacag agagcgtcac cgtcagcgta tccgccgtta
    21181 aatgaaacgc ctgacttacc cgatagtgat agacaccgct gcgatgttca tacaccagac
    21241 acaaactatc atcgctgtgc gagacacatt gccactcgcc cagccagcca tcgccgtgta
    21301 gatagtgcgc atcccactca acattcggtt gcagttgata ctcacgcccc tgccagacaa
    21361 aacggttgcc gctcacccgg tttgcaaatg gcaccagcgg aaaacatgag gcatcggttg
    21421 ccacaccgct ttttttacca ggacgtaaga gcggcgtcgt atcgcgccag aagccttcga
    21481 ttacgccgcc ctggtcagaa acgtccagct ttagcgaccc gtgggataag gtatagatgg
    21541 tcatccgcta actccttaat ccgggaagtt aatcacaact ttcccgcatt tgccgctcgc
    21601 catcagcaca taggcatcac ctgcctgttc tagcgagaag cgatgggtaa tggcgttacg
    21661 cggccacagc ttccagtcag tcaaatcgtg ggcgcatttt tccatatgga acagactggt
    21721 cacccaggag ccgataatcc gccgttgatg gtgcatcaga tcagcgctga cctcgaattc
    21781 cacttttccg gtttcaccaa tgtaaaccac ccgcccccag tcagcagtgg attgcagtgc
    21841 cagcaagcga cctgcggcat taccggaaca atcgagcgca acatccgcgc caccgtgggt
    21901 aagttcagca attatctgcg gcagaccttc ggtggttgct aaatagccgt gatccatccc
    21961 ccctaactgt tttgccatcg ccagacgttc cggcagcata tcaacgccga tgatccgttt
    22021 tgcaccgcga cctttcgcca gcatcatcgc catcatgccg accggcccca gaccgaccac
    22081 cagcacgtta tcgctgccgg aaacttcgcc gcgcaaaatc ccttcatagg ccgtgccaac
    22141 gccgcaactg ataaacgcac catcttcgta gctcagcgca tccggcagga ggatcagatc
    22201 tttttcttcc gccagcagat attcggcatg accgccgtca cgctgccagc cgtaagccgc
    22261 ttttcctttg ccagtacaag agataggaaa gccgcggcgg cagttcgggc aaaaaccaca
    22321 gccagaaatg tgatacacca gcacgcggtc gccctcttta aaatggcggc agccttgccc
    22381 catcgccaca atctgcccgc acggttcatg accgttgata aatccctggt ataacggttt
    22441 atcgggtgcc gccgccgtgg cacggtgttg atgatagata tagtggacat cgcttccgca
    22501 aatcccggag gatttcattt tgatcagtac ctggttaatc cccggcgtcg gcaccgcaac
    22561 ttcccgcaga tcgacggttg aatttcctag taaataagca gccagcatcg ttttcataaa
    22621 tccctcatta acaatacgat taattttcat ccctgcagca cccgcgccag gggcgttaag
    22681 gtttagcgtt tcgctttact gcttcgctgg gtcagcaaga tattcgccag caccgccacc
    22741 acgatgatga cgccgcgtac cacctgctgg aaaaaggagt taataccgag cagcaccaga
    22801 ccgttaccga ttagcgtaat caccagcaca ccaagcaatg taccgaacag ggaaccgcga
    22861 ccgccggaaa gtgccgtacc gccgaccacg accgcggcga tgacgtcaaa ctccagaccg
    22921 tttgcggcac ctgcgttacc ggaaccgagg cgtgccgcca acaaaatgcc ggtcaccgcc
    22981 gctaataatc ccgaaagggt aaagataaga atgcgcactc gacgaacgtt aataccgcac
    23041 aactgcgccg ccgtggcatt accgccaacg gcaaaaaccg agcgcccgaa ggcggttttt
    23101 cggctaatga acacaaacag cgcaaacaac accatcatga tcagcgcgga tactggcaca
    23161 ccgagaaatt gtccgcccag ccagtccagc acctcgtttt cgtcaatcgg cactggcagt
    23221 gcgttggtca taaacagccc cattccgcgc agagcgctcc acagccccag tgtggcaacg
    23281 aaacttggta cgttaaacac gccgcgcagc accccggcga gcgttcccat cagcgcgcct
    23341 aacagcaaca ccagcagaca agcaatcgcc aacggaactt caaattgcag caaaaatgcc
    23401 aggcacaccg agacaaaagc caccatcggc ccaacgctga catcaatttc accggagata
    23461 ataatcagcg tcatcgccca ggcggcaatc ccaatggtgg cggcatcgcg cagcacgttc
    23521 atctggttat tcaatgagat aaagccaggc gcgttcaggg agaagaccag ataaagaatg
    23581 gcaatcacca ccagcaaacc gatctcatta atatggcgac tgacaaattg tttgagcgag
    23641 acgctcttgc cctgcggcaa tggtaatgac gaagcataca tcttcaggtt cctcggtaaa
    23701 attcatcagt gcacagacag aatggcggac atcagctcat ccacattgac cggcgagtga
    23761 aactcctgcg agaacgtgcc gtgctgtaat aacaggatgc ggtcacacac cagcggcaat
    23821 tcctccactt cactggagat aaacaccacg ctttttcctt cggcagccag ctcacggaca
    23881 atacggtaaa tctgctgttt ggcttcgata tcgacgccgc gcgttggctc gtcgagcaac
    23941 aaaatctggc tggcggcata gacccaacga ccgatcacca ctttttgctg attgccacca
    24001 gaaagcgtgc cgatgggtgt ttcgctactg gcagccttga ccgtcatccg ctgcatcacc
    24061 tcttcggtca ggcggcggat ggtggaccat tgcagcacac cgttggtgct gattttttgc
    24121 cgattggtca gcactgtatt ttcgtcaacg cctagccagg gaatgatccc cgcttctttg
    24181 cggttttctg gcgtatagcc aatgccgcgt ttcagcatgt cgccgtaatc ggggcgcatg
    24241 attttctcgc cgttgataac aatttcgccc tgttcatacg tctccagccc aacaatcgcc
    24301 ttcagcaatt cactgcgccc tgcccccagc aggccagcaa tgccaagcac ttcgccacga
    24361 cgtagcgtaa agctgatatc ctccagcttg ggcttatggc gtaacgcacg gacttccagt
    24421 acggcctgat ccacaatttc ctgaggggca accggcgcaa tatcaacgtg atcacgccct
    24481 agcattagcg acacaatatg atgcgtggag gtgttttcga gcatcacatc gcccgccacc
    24541 tgaccatcgc gcataacggt ggcataggag gcaatgcggc ggatttcttc catccggtgg
    24601 ctgacataaa tcaccgccac gcccagtgcc gacatctttt tcaccgcact gatcaccagt
    24661 tcaacttctg cactggcaag cgagctggtg ggttcatcaa gaatgaccac gcgcggctcg
    24721 cccttcatca cccgtgcaat ttccaccagt tgcttttgcg ccgggcttag cgttgaaaca
    24781 agttgttcag gactaacgtc aacgcccagc gcctgtaagc aacgttgggc atcctgcgcc
    24841 atttgcaggt aatcaatcat gccgttgcgg cgaggccatt gaccaaggca gaggttttct
    24901 gccaccgtca gcccttccac cagacttaat tcctgataaa ccgcacgaac ccccagttca
    24961 gcggcacggc gagtcaacgt agcttcgtca ccttccagtc gcgtctcgcc aatccagata
    25021 tcaccgctat ccgggcgttc gctgccggta agcattcgaa tgagagtcga tttgcccgcg
    25081 ccgtttttac ctaacagcgc acgaacttcg cctttattga gcgtgaagtt aacgttatcc
    25141 agcgcaacga cgccggggta acgcttattt cctgccacca cttttgctac cgggactgcc
    25201 tctgttgccg tgaacatagg gacctctgcg aatcagcgat tagggcagac catcaacgtg
    25261 cgttgccagc cactgtttac catcttccgt tttggtatag agatcgattg gcacctgaat
    25321 cactttttca ccgtcggctt gtttattgat aaccttcaat gtttgcgcga aaacagcatt
    25381 gcccattttc ttaccggaaa tatccactac cgctttcagc acctgattgt tttccagctc
    25441 ctgagcaatt tcggttgtca tatccgaacc gaaaacagca atttttccgg cctgattttg
    25501 attacgtacc gcttttaccg cgccgagtgt cgcaccgccc gactccccca taatggcgtt
    25561 gagatccggc gtggagataa tcagtttttc accaacggaa atcgctttat ctaaaacagt
    25621 cccttcctgg ttagcgacaa tttgcgcgcc gggaacgcgg gattttaata cttcttcaaa
    25681 tcctttacgt cgctgcacac aaacttcaaa ggcttcgcaa ttgatgacgg caattttcgg
    25741 ctggtcaatt ttattggcaa taaaataatc ggcggcagcg ttacccagtt tttaccaaat
    25801 tccagtggat cgccgaccag atacgccgag acatatttat cgacaccctt ttgattaata
    25861 caggtgttgt agcaaatcac cggaataccc gcttcactgg cgcgacgaac ggtacggcta
    25921 ctgccatttt cagacactgc cgataaaata atggcatcga cattacgcgc cacgagggta
    25981 tcaacaaagg tactttcttt cgaaatatcc ccctgggcgt tagtttcaat taactgtacc
    26041 tgtactgaag aatcttttgc cgcatcctga acgccctgac gcactccagc gtaatatccc
    26101 tgggtatcaa ggtatattgc accaatggtc atttctttat ccgcagccct ggcaaatagt
    26161 gcgctaccta atagcgtagc cattaataat agattacggg ttgttctcat ttttttaggc
    26221 atagtgcctc ctgtagggtt tttattaaca acggcttatt ctaattattt tgtgatgagc
    26281 ggcagcgcgt gccgctcaaa tattacagtt aatagggttt agtgaacgta gttaaaaata
    26341 aaaggcatca cagtggtgaa taatgccgct ttcggccagg ccggattaat cgccgtcaat
    26401 tcagcgcgag cagactcata ttgcgcaacc tccccgagtc ccgcggacgc cagcatcgcc
    26461 accatcaggc atttttcttt atgctgctgt tgtaaatcgc catacagcga taacaggtca
    26521 ggttgtgaaa cagcaaagaa atccgcctca atactggttt tcgccatctc ttgcgcccac
    26581 tgtttcattt cactaaacag ttgctgtgcg gtttgctgtt cacccagcag tcgcagcgcc
    26641 attccttgcc agaagagata atcaaccggc tgatcgttgt aataactgtg gatgttaatg
    26701 gtgcgatcgc cggtcgccgc cagacgtaaa caacgcatcg cttcagtttc atcgccctgc
    26761 gcgttggcgc atatcgcctg ccagaaccag atgtcgttat cagtttgccc cggtaaacgg
    26821 ccttcgctta aattctccgg ataatacagc gcggcatgaa gcagttcgct ggcctgctgc
    26881 ggctctctgg catcaagatg ctgccaggcg cgtaataact ggttgaggat aaactgactg
    26941 gtgaccttcc cttccccgcc ttcccacggg tggaatttac gcgtggcgag aatgtccgcc
    27001 gctttgtctg cctgacccgt gagatgccac aaattgagca gttctgcggt catgtcgtcg
    27061 cgtttcagcg caatttccag attattttcc agacgcgcca gtcgtttctc cggtgtggct
    27121 ccacttaact tatccagcaa atcccgttcg aaaagcagac gtgcatcctg cggcgcaagc
    27181 tgataagcat tatcaagata acgcgcggcc agctcataat cgtgtttctt attccacgca
    27241 tggatcgcta acccgcgcca gccgtcggca aactccggcg acatctctac gcaacgttgc
    27301 cataaggcaa tggctttgtt gtagctacgt ttgttgtagt agaagcaggc cagtaaatgg
    27361 cgagcaaacc aacactcttc aatactctcc agcgccgcca cttcttccag cgtattcggg
    27421 aaacggacaa actgcgggaa gacatcaatg gcttttgcga ccagttcgcc acgttcggct
    27481 ttcggcagca agctggcttg caggtaaagc ggcagcgtgc gctggcagtc cagtgcgttc
    27541 agcatctctg ccgccagggt gggcattccc cagttaatca gttgcccggc ggtcatcaga
    27601 gcgttaacgt cgcgtccctg acacagaccg cgccactgcg cgagggctga ttcgctacga
    27661 ccatcgaacc agttcagcca ccacagagtg gcgttcagcg gataatcacg cagcagtttc
    27721 tcgcgctgca aacgcgcgtt gtcctgacga ccacttaaca ccagcagcag attatgcaag
    27781 caaagcactt cctgattggt tgggcaggca cgaagacttt gttggcaaaa atccagaccc
    27841 gcgtcgaagt tatcattacg cgccgccagt cgtgccagac cataatagcc gcctgcttta
    27901 ctattgccgc tccagaccgc acgccagaaa tcctcttcgg cttgttgata ttgtccctga
    27961 cgttcgtaag cactggcgcg aatcaaactt gcttgtccac actgcggttt tttgttcagc
    28021 gcatgtgcac gtttcagcgc ctgactggca tacgccaccg cttgcgggaa atctgcgcgg
    28081 ttatattcca gcatcgccag cgccaggtta cagcgataat ccagcgggtc cagcgccacg
    28141 ccgcgcaggt agtaatcgaa cggtgaacgg ctggcgtgat gatattgctc cagatgctga
    28201 ccgataaacc aggcttcatc tgtactggta atgtcttgtg ctgccagtgg cgctttggcg
    28261 acgtccggca acggcaactc ctgcggctga tgttcctgat aactcagtac aatattgccg
    28321 tcggcatcgg agagttcaat ggtcagtctg tcaggattga taccgtgcaa cacgccctgg
    28381 atggcggtcg ctggtgtcag ggccacggca tcatcgagca acgcgttgca tttgccgatt
    28441 tcgcggatcg ccagacggta tccgttcaac ggagagatgg catacagccc ccactcaatc
    28501 ccccgctcac tacgctggag ttttatcacc gcatcgcggg aggcattttg caccatgccc
    28561 agagaatgat aaggcaggaa atactgctcg aaacgcttct cttcgtaagc atcaagccag
    28621 gtaaaatcag gctggttatc ggcaaaaata ccggtcatca gttcgatata cgggccgtta
    28681 ttgtcggtca gactcttatc ccacgcctgg ccaaattcac tgtgtcccca actccactgt
    28741 tttttacctg gcgcaatatg gtggttggca acgtgcagca aaccgccatc ttcatcgtga
    28801 caccacgcgc cgacaaaatc gtactgcgat ttttcggcca tatatgaggt tggaacaggc
    28861 acatttttat agcgagaaat gtccactccg gcggagtagt ccactttgta gtaagtgccg
    28921 gtggcgatgg ggaaagcgga gacggcccgt ttgccgtgat caaacaccgc agtcacatcc
    28981 ggtgggaaaa cgctctgatg cccttcaccc cctttcactg ccgggttgac ccaccacaag
    29041 aaatgacgcg gcgtggcgtt gccgttatag acgcggctgg cgatttccag tgccgcccgg
    29101 tcagggcgca gggtgaaacc tgtcatcacc tgtaaaccat gcatcggctc cgtttcgccg
    29161 acccacaccg tctgtgcacc gtcttcatgg gcttcgaggg tgaaatcaac gggcataaag
    29221 gtggtcgggc gatggtgttg cggccagtta aactcaatcc cgccggagat ccacggtccc
    29281 agcatcccca ccagcgcagg tttaatgact tcattgtgat agacaaaatc gcgctgtttc
    29341 actttatccc atgcgcgatg cacccgaccg cccagttccg gcaggatcat cactttgatg
    29401 tagtcgtttt ccagccacac cgcctgctag gatttcaggg ttttctgctc gctcagcgta
    29461 tcggtcacgc cgtagggata aaccgcgccg gacgatccct gataaacgcg attttccagg
    29521 aacatgggat gtatatcctg cggcccggtt tcataggtcg ggatctcaac gcgctcttgc
    29581 cacactttta ctggagtcat ggtgccctca atattaacaa gacatactga attaaaagat
    29641 ttgtggcagt gtattgaaca atctggcaat gttttcgcgg aataatcacg caattaacta
    29701 aacaaggttt agtgaagatg agagcctgca ttaacaatca acagattcgc caccataaca
    29761 aatgcgtgat tctggaactg ctgtaccggc aaaagcgcgc caataaatca acgctggccc
    29821 ggctggcgca aatttcgatt ccggcagtca gtaatatttt gcaggaactg gaaagcgaaa
    29881 aacgggtggt gaatattgac gatgaaagcc agacgcgcgg gcatagtagc ggtacatggc
    29941 tgattgcgcc ggaaggtgac tggacgctgt gcctgaacgt gacgcccacc agtattgagt
    30001 gtcaggttgc taatgcttgt ttaagtccga aaggcgaatt tgagtattta cagattgatg
    30061 taccgacgcc gcaggcgctg ctgtccgaaa tcgaaaaatg ctggcatcgc caccgtaaat
    30121 tgtggccgga ccgcaccatc aatctggcgc tggcaatcca cgggcaggtt gatccggtga
    30181 ccggcgtatc gcaaaccatg ccgcaagcgc cgtggacaac gccagttgag gtgaagtatc
    30241 tgctggaaga gaagctgggc attcgggtgc tggtcgataa tgactgcgtg atgctggcgc
    30301 tggcggagaa atggcaaaat aattcgcagg aacgggattt ctgcgtgatc aacgttgatt
    30361 acggcattgg ctcgtcgttc gtgattaacg agcaaattta tcgcggcagt ttgtatggta
    30421 gcggacagat tggtcacacc atcgttaatc cggatggagt cgtctgcgac tgtggacgtt
    30481 acggcagcct ggaaaccgtc gcctcgttaa gtgcattaaa aaaacaggcg cgggtatggc
    30541 taaaatcaca accggttaat actcagcttg agcctgaaaa actgacaacc gcgcagttaa
    30601 tcgctgcctg gcagagtgga gaaccgtgga tcaccagctg ggttgatcgc tctgccaatg
    30661 ccattggttt gagtctgtat aacttcctca acatcctcaa tattaatcag atttggttgt
    30721 acggtcgcag ttgtgccttt ggtgagaact ggcttaatac tattattcgc cagacaggat
    30781 ttaacccgtt cgaccgcgac gaaggaccga gcgtgaaagc gacgcaaatt ggcttcgggc
    30841 aattaagccg cgcacaacag gtgctgggaa ttggctattt gtatgttgag gcgcagttac
    30901 gacagatttg atggcgcgat aacgtagaaa ggcttcccga aggaagcctt gatgatcata
    30961 aacgaaaaat tgcctgatgc gctacgctta tcaggcctac acggagattg caatatattg
    31021 aatttgcaag attttgtagg cctgagaagg tgttcatgcc gcatccaaca tgaacaacga
    31081 gcacattgtc agcaaatcac cgtttcgctt atgcgtaaac cgggtaacgt gcgcagatgt
    31141 cgagaacttt acctttgatg cgctcgataa cggcttcatc attgatgctg tccagcacgt
    31201 cacacatcca gccagccagt tctttcgctt cggcttcttt aaagccgcga cgggtaatcg
    31261 ccggagtacc tacacgaata ccggaggtca caaacgggct cttcggatcg ttcggtacgc
    31321 tgtttttgtt gacggtgatg ttagcacggc ccagagcggc gtctgcttct ttaccggtca
    31381 ggtttttatc aaccagatca accaggaaca ggtggttatc agtgccgccg gaaaccactt
    31441 tgtagccgcg ctcgaggaac acttctacca tcgctttagc gtttttcgcg acctgctgct
    31501 ggtaagtttt gaactcaggc tccatcgctt ctttcagagc aaccgcttta ccggcgatta
    31561 cgtgcatcaa cggaccgccc tgaccaccag ggaaaacggc agagttcagt tttttgtaca
    31621 gctcttcgct accacctttc gccaagatca ggccgccgcg cggacccgcc agggttttgt
    31681 gagtggtggt agtaacaacg tgagcatgag gaaccgggtt cgggtagacg ccagcagcaa
    31741 ccaggcccgc aacgtgcgcc atatcaacga acagataagc accgatgctg tcagcgattt
    31801 cacgcatttt cgcccagtca accacgccgg aatatgcaga gaaaccaccg ataatcattt
    31861 tcggcttgtg ttctttggct tgtttttcca gatcggcgta atcgatatga ccggtagcat
    31921 cgataccgta aggaacgatg ttgtacagtt taccggagaa gttaaccgga gaaccgtgag
    31981 tcaggtgacc gccatgcgcc aggttcatac ccagaacggt atcacctggt tccagcagcg
    32041 cggtgtagac cgcaaagtta gcctgggagc cggagtgcgg ctggacgtta gcgtagtcag
    32101 cgccgaacag ttctttcgca cgatcgatcg ccagttgttc aacgatatca acatactcgc
    32161 aaccgccgta gtagcgtttg cccggataac cttcagcata tttgttggtc agctgagaac
    32221 cctgcgcctg cattacgcgc gggctggtgt agttttcgga ggcgatcagt tcgatgtgct
    32281 cttcctgacg tactttttcc tgctccatag cctgccacag ttcggcatca taatcggcaa
    32341 tgttcatttc acgctttaac atccgcatct cctgactcag ctaacaataa aatttttggc
    32401 ctttataggc ggtcctgttg gacaacggcg aacagtataa ccgaatcatt gtgcgataac
    32461 aggtcttgac aaaggaattt acgcaaacga ttaccttcag gctacgcaag gctttggaga
    32521 ataaagagct tgcaaccgga aacggatttc ttttcaggtt tgtgatgcaa atttttcact
    32581 tcatcacatt ctttctgaaa aacaccaaag aaccatttac attgcagggc tattttttat
    32641 aagatgcatt tgagatacat caattaagat gcaaaaaagg aagaccatat gcttgacgct
    32701 caaaccatcg ctacagtaaa agccaccatc cctttactgg tggaaacggg gccaaagtta
    32761 accgcccatt tctacgaccg tatgtttact cataacccag aactcaaaga aatttttaac
    32821 atgagtaacc agcgtaatgg cgatcaacgt gaagccctgt ttaacgctat tgccgcctac
    32881 gccagtaata ttgaaaacct gcctgcgctg ctgccagcgg tagaaaaaat cgcgcagaag
    32941 cacaccagct tccagatcaa accggaacag tacaacatcg tcggtgaaca cctgttggca
    33001 acgctggacg aaatgttcag cccggggcag gaagtgctgg acgcgtgggg taaagcctat
    33061 ggtgtactgg ctaatgtatt tatcaatcgc gaggcggaaa tctataacga aaacgccagc
    33121 aaagccggtg gttgggaagg tactcgcgat ttccgcattg tggctaaaac accgcgcagc
    33181 gcgcttatca ccagcttcga actggagccg gtcgacggtg gcgcagtggc agaataccgt
    33241 ccggggcaat atctcggcgt ctggctgaag ccggaaggtt tcccgcatca ggaaattcgt
    33301 cagtactctt tgactcgcaa accggatggc aaaggctatc gtatcgcggt gaaacgcgaa
    33361 gagggtgggc aggtatccaa ctggttgcac aatcacgcca atgttggcga tgtcgtgaaa
    33421 ctggtcgctc cggcaggtga tttctttatg gctgtcgcag atgacacacc agtgacgtta
    33481 atctctaccg gtgttggtca aacgccaatg ctggcaatgc tcgacacgct ggcaaaagca
    33541 ggccacacag cacaagtgaa ctggttccat gcggcagaaa atggcgaagt tcacgccttt
    33601 gctgatgaag ttaaggaact ggggcagtca ctgccgcgct ttaccgcgca cacctggtat
    33661 cgtcagccga gcgaagccga tcgcgctaaa ggtcagtttg atagcgaagg tctgatggat
    33721 ttgagcaaac tggaaggtgc gttcagcgat ccgacaatgc agttctatct ctgcggcccg
    33781 gttggcttca tgcagtttac cgcgaaacag ttagtggatc tgggcgtgaa gcaggaaaac
    33841 attcattacg aatgctttgg cccgcataag gtgctgtaat ttgatgttgc cggatggaaa
    33901 catccggcaa cccttgacgc ggtttaaatt gccgcgtcgt cctcttcacc ggtacggatg
    33961 cgaatgaccc gagccacgtc aaagacgaag attttaccgt caccgatttt gccggtttgc
    34021 gccgtgcgaa taatggtatc gacacaggta tcgacaatgt cgtccggtac gacaatctca
    34081 attttcactt tcggcagaaa atccaccata tactccgcgc cgcggtacag ctcggtatgg
    34141 cctttctggc gaccaaagcc tttcacttcg gtcaccgtca tgccggtaat accgacttcg
    34201 gccagtgctt cgcggacatc gtccagcttg aagggtttta taatcgcatc aatctttttc
    34261 atgctattcc ttgaaaaggt tgcctgtctt ttgatctgct aaacgtaaca cataacgcca
    34321 attcattcct tgaaatcgtt tgcatccagc tcgtgtcggg aaagcagttt ataaaattct
    34381 gtccggttgc gccccgccat tctcgccgcg tgggtgacgt tgcctttggt gatttgcagc
    34441 agcttacgca agtagttgag ttcaaaatga ttacgtgcct caacaaaggt tggcagcgcc
    34501 gtattttcac cctccagcgc ctgctccacc agcgcatcac taatcaccgg agatgaggtc
    34561 agcgccacgc actgttcaat cacgttgacc aactggcgca cattacccgg ccagctcgcg
    34621 gtcatcaggc gtttcatcgc atcggtagag aacgcgcgga caaacggttt atgtcgctct
    34681 gccgcctggc gcaacaggtg atttgccagt agcggaatgt cttctgtgcg ctccgccagt
    34741 gccggaattt tcaggctgac aacgttgagg cggtaataga gatcttcgcg gaattccccg
    34801 cgcgccatcg cttttgacag atcacggtga gtggcagaaa taatccgcac attgatatca
    34861 atatcgcggt tactgcccag cgggcgcact ttacgctcct gcaacacgcg cagcagtttg
    34921 acctgtaacg gcgcaggcat atcgccaatt tcatcgagaa atagcgttcc gccttccgcc
    34981 gcctggaata aaccttcgcg attgctgaca gcgccagtaa acgcgccacg cgcatgacca
    35041 aacagctccg actccagcaa ttgctcgggc aatgcgccac agttaatagc aataaatggt
    35101 ttgctgttgc gcgggctggc gttgtggata gcctgggcga aaatctcttt cccggtgccg
    35161 ctctgaccgt taatcaaaac gctgacgtct gattgcgcca ccagccgcgc ctgttccagc
    35221 aaacgcagca tcagcgggct gcgggtgaca attgcctcgc gccagcgttc atcggtggct
    35281 ggcgcggatt gctccagcgc atcgtcaatt gcctgatata gcgcatcttt atcgacaggc
    35341 ttggtgagga aactaaaaac gccctgctgt gttgcagcaa cggcatcggg gatagaacca
    35401 tgcgcagtaa gaataattac tggcattccc ggctgcactt tctggatttc agcaaatagc
    35461 tgcataccgt ccatttcatc catccgcagg tcgctgataa ctaaatctac tttttcgcga
    35521 ttcagtaccc gtaatccttc agcgccactt tccgccgtga ccacactgta gccttcgctg
    35581 gtcaggcgca ggccaagcag tttcagcaat cccggatcgt catcgaccaa taataaatgc
    35641 gcaggtttat ggctcatcag gagtgacctc atgggtggat ggcgcgggct tttcactctc
    35701 gtgcggcgta tccggcgaga aatttccagc cggtttgcgg gtcgagagct ggcgttcaat
    35761 atcggtcagg ttttccagct tgcgggtggt gagttccagt tgctgctgta gaacgtgatg
    35821 ttgctggcgc aatgtatcca gctcgctgtc gctggactgc tggagtttac tgtaacgttg
    35881 gcgctcttcc gccagttgca gttgcagcgc ctgaccgtcg cgccagagtt gatacagtgg
    35941 gcgaacctgt gccgggatct cggtacttaa cgcttcaata cgcgcgacca gctggcggcg
    36001 ctcatacggc gtaattttgg cgtcggcgag caaaatccct tgtttaaagg tattttgcca
    36061 gctgccgtcg tcatattggc gagcttgctg acgcgactgc gcaggcatta aacgatcagc
    36121 acaatccatc gcccgcagcc agtaaagcgg attggtttcg gttgatttac cttgcagcgc
    36181 ccagatgtcg ctacattcag tagaaagata gtcagccagt tgataaaccg gaattttttc
    36241 ttctgctggc gtatcaatgg ctggcttatt gtgattctgc acgcaaccca gcaatgccag
    36301 acatggcagc cctgccagcc acagccgtcg gggcaataat cgttgaaaaa tgtgtcgcat
    36361 attcaccaga cttaaagcct atcccagtgg gcgtaattgt tgcagacagt ctggacatgg
    36421 actgcgcgga gaaaccgggg cgtacatatc gtacgtgaag atttcgagca ctacccgggg
    36481 ccgaactgac aaataaaata gcctgatagg ataggctgtt agcattattt cgtgtttttc
    36541 gacgacggta attcaatgcg gaaacaaacg tcttgcccgc tctcgtcgac cagatacagt
    36601 tccccttgca tacggcgaat acaatccctg gcaatgctta atcccagacc gctgcccttc
    36661 accgcccctt ttcgctggtg gcttccctga aaaaagggtt cgaagatcat ggcgcgttcc
    36721 tcttgcggaa tgggcgtgcc tgtattgatg acatcaatat aaacccgcgc accatgtaaa
    36781 ctgctgcgaa ggcaaatgtt accggattca gccccgtagt gcaccgcatt ggagtaaaga
    36841 ttatccagta cgctcatcag cagcattggc tccgccaggc aagctgttgc tttgagatcg
    36901 acgtcggtat gcatcatttt agcccgtgcg ggcaggctat gagcagaaac cactgtctcc
    36961 accagcggtg ctaattcaac attctccagt tccaccgcac tgtccgcctg tttacggttg
    37021 taatcaagca gttgttcgat cagtttttgc aaattgcagc tgctgctatc aagaatgctc
    37081 accacctctt tttgctctgg cgtaagcggc ccgacaacct gatcagccag taattcagtg
    37141 ccctcgcgca tactcgccag tggcgttttt aattcatgag ataaatgtct taaaaattga
    37201 tggcgttggg attccagcca tgacaggcgc tcacttaacc aaagaatacg ttgcccaacc
    37261 gagcgtaact cgctcggtcc acagaacgaa acgctattgc ccagagaacg cccttccccc
    37321 agacggttga tcatgcgctc gatatttttc accggcccga taatcatccg cgtgaaaagt
    37381 agcaccatca ccagactcac cagaaatagc accagcgatt gccaaccaaa atattgccca
    37441 cgttcggcga tttcacgctg aagttgctgc ccacgagaga acaccactgt gcgcgtggcc
    37501 tgcaccattt cggtattggc actggcaaag gcttcaagac gcgcggcggc ggcagcatcg
    37561 ggaccactgt tgttacactg aagttgggcc agattgttca aatcctgacg caatgcctgg
    37621 taaagtttat cgtctggcag tacgcctgcg tgggcatcga gcatttcgct gtaacgcttg
    37681 cgctggcttt gataaacctt cgcaagcgta gggtcgtcca gcacgcaata ctgacggtag
    37741 ctacgctcca tctccagcgc cgcgttggtc atcgcttcac tacgccgggc atcaataagc
    37801 gtagtgcggt taaccagcgc cgcctgatcg ctaagcgcat tcaggctttg ccaggcttgc
    37861 catgccagca ccaacagggg tagtagaatt agcaaaaatg ccagcattac cagttgtcgt
    37921 aatgagcggg gaaaaacggg ccagcgtttc aaggtgttac tctcgtcaga cgcgaatagc
    37981 ctgatgctaa ccgaggggaa gttcagatac aacaaagccg ggaattaccc ggctttgtta
    38041 tggaataagg cggtgcctaa ctcgacgttt cgcccgatgg ttgatatagc tacgctgata
    38101 tcagaagttg gacggcaggc accttgttgt gcgtcattcg aattttatgt agcacgtccc
    38161 gaaggggctg acataagtcg gtgaatgagc cactgcttac tattatgcag taactgtgcc
    38221 aataaagaaa atagtttggt aacgtactga ttatacgttg ctttgaggga tttatggctt
    38281 cttcgctatt tgaatgtaaa tcagccactg tgtcgctaaa aagagacaac ttaagataaa
    38341 cttattagat tgcaataaaa tcaatcattt aagtgtcgcc agaaagcgac acggtaaacc
    38401 acccattgtc gtgatttaca gacacaaaaa agctcccgga gttgggagct tatgatagtg
    38461 gttggtgctt aatgccgcat ccgggctgca aaaccaatga actgatgact taccccaact
    38521 gcttacgcgc attgcggaaa atgcgcatcc atgggccatc ctcgccccag ttttccggat
    38581 gccaggagtt gctgacagta cggaaaacac gttccgggtg cggcatcata atggtgactc
    38641 gaccactttc agtcgtaacc gccgtaatac cgttcggtga accgttcggg ttagccgggt
    38701 aggtttcagt gactttgccg aagttatcga catagcgcag tgccaccagc cctttgcttt
    38761 ccagtgccgc cagatgcgcc gcatcacgca cttccacgcg cccttcacca tgagagacag
    38821 caatcggcat ttgcgagccc accatcccct gcaacagcag agacgggctt tgggttactt
    38881 caaccaggct gaaacgcgct tcaaagcgat cggaggtgtt acgcacaaaa cgtggccaca
    38941 actcactccc cgggatcagt tcacgcaggt tagacatcat ctggcaaccg ttacataccc
    39001 ccagggccag cgtttgcgga cgatggaaga aggttgcaaa ctcatcgcgt acacggtcat
    39061 tgaacaggat agacttcgcc caaccttcac cggcacccag cacatcaccg taggagaaac
    39121 ctccgcacgc gaccagggcg tgaaaatcct ccagtcccgt acgtccagtc agcaggtcgc
    39181 tcatatgcac gtcgatagca tcaaagcccc cacgttggaa agctgccgcc atttcaacat
    39241 gcgagttcac gccctgctca cgcagtacgg caactttcgg acgtgcgcca gtggcaatat
    39301 acggtgctgc cacatcttcg ttgatatcga acgacagttt tacattcagg cccggatcgg
    39361 cgtcgttaga tttcgcctga tgctcctgat cggcacactc cgggttgtca cgcaggcgct
    39421 gcatctgcca ggtagtttct gcccaccaga cacgcaacgt agtgcggctt tcgctgaata
    39481 cagtctgccc gttggcggta atcacaaaac ggtcaccgga aaccgcctgc ccaacataat
    39541 ggacgcaatc cgcaagccca tgctgtgcca gtacggactc gaccgcttca cggtcagcgg
    39601 cacgaacctg aatcaccgca cccagttctt cgttaaacaa cgccgccagg cgatcgtcac
    39661 ccagagaggc gatatccgcg tcaatgccac aatgacctgc aaaggccatt tccgccagcg
    39721 ttaccagcag gcctccatca gagcggtcgt gatacgccag cagcttacgc tgtgcaacca
    39781 gcgcctgaat cgcgtcatag aagcctttca gttggacaac atcgcgtaca tctgccggtt
    39841 tgtcgccaag ctgacgataa acctgcgcca gcgccgttgc gcccagcgcg ttattgcctt
    39901 tgcccaaatc aatcagcagc agtgcgttat cttcggtaca aagctgcggc gtgatggtgt
    39961 gacgtacatc ttccacgcgg gcaaatgcag aaatcaccag cgacagcggc gacgtcattt
    40021 cgcgctcttc gttaccttcc tgccagcggg ttttcatcga catagagtct ttacccaccg
    40081 ggatcgtcag gcccagcgcc ggacaaagtt cttcacccac ggctttaacg gcttcataca
    40141 ggcccgcatc ttcaccaggg tggcctgccg ccgccatcca gttggcggaa agtttaatgc
    40201 gtttgatatc gccaatctgt gttgcggcaa tattggttaa cgcttcaccg accgccagac
    40261 gggcagaggc ggcgaaatcc agcagcgcaa ccggcgcacg ctcgccaatc gccatcgctt
    40321 caccgtagta gctgtcgagg ctggcggtag tgaccgcgca gttagcgacc ggcacctgcc
    40381 acggccccac catctgatcg cgcgccacca tgccggttac gctgcggtca ccgatggtca
    40441 ccaggaaagt tttctccgcc acggtcggca gatgcagtac gcgtttcacc gcgtctgcaa
    40501 tggtgatccc ttcacggacc agcgcatcgc ccttcgcttt cagcgtttgt acatcgcggg
    40561 tcatcttcgg cgttttacca agcaggacgt ccagcggcag atcgatcggc tgattatcaa
    40621 aatgacgatc gtgcagagaa agatgcagtt cttcggtcgc ttcaccaatc accgcgtagg
    40681 gtgcgcgctc acgcttacac agttcgtcaa acagcggtaa ttgatcggca gcaaccgcca
    40741 gcacgtagcg ttcctgggat tcgttacacc agatttccag cgggctcatg cccggttcgt
    40801 cgcttagaat atcgcgcagt tcaaatttac cgccgcgtcc gccgtcgctc accagctccg
    40861 gcatcgcgtt agaaagaccg cccgcaccga cgtcgtggat aaacagaatt gggttagcat
    40921 caccaagctg ccagcaacgg tcgatcactt cctggcaacg acgctccatc tccgggttgt
    40981 cgcgctgtac ggaagcaaag tcgaggtcgg catcagactg accagacgcc atggaagacg
    41041 ccgcgccgcc gccaagaccg atgttcattg ccgggccgcc gagaacgacc agcttcgcac
    41101 cgacgttgat ctcgcctttt tgtacgtgat cggcgcgaat gttgccgatc ccgcccgcca
    41161 gcatgatcgg tttgtgataa ccgcgcagct cttcgccatt gtggctgttc actttttctt
    41221 cataagtacg gaagtagccg ttcagtgccg gacgaccaaa ttcgttgtta aacgccgcac
    41281 cgcccagcgg gccttcggtc atgatgtcca gcgcggtaac aatgcgctca ggcttaccga
    41341 aatcttcttc ccacggctgt tcgaagccag gaattcgcag gttggatacg gagaaaccaa
    41401 ccaaaccagc tttcggcttc gcgccgcgcc cggtggcacc ttcatcgcgg atttcaccgc
    41461 cggaaccggt cgccgcgccc ggccacggag aaatcgccgt cgggtggttg tgagtttcga
    41521 ctttcatcag gatgtgcgcc ggttcctgat ggaaattgta gcggcccgtt tcgtggtcag
    41581 caaagtagcg gcccacttca gaaccttcca ttacggcggc gttatcttta taagcagaga
    41641 gaacgtgatc tggcgtggtt tcgaaagtat ttttgatcat cctgaacagc gatttcggct
    41701 gttgttcacc gtcgataatc cagtcggcgt taaaaatttt gtggcggcag tgctcagagt
    41761 tcgcctgagc gaacatatac agctcgatgt cgttcgggtt acgaccaagc tttgtgaaag
    41821 catcctgcag atagtcaatt tcatcttccg ccagagccaa gccaagacgc aggttagcgt
    41881 cgatcagcgc ctgacggccc tgccccagca aatcaacgct tgtgaccgga gtcggttgat
    41941 ggtgggcaaa caactgctct gcatcatcta aatcaaaaaa gaccgtttcc atcatgcggt
    42001 cgtgcagttc agcggtaacc tgctgccatt gttcattggt cagcgtaccg gcttctatat
    42061 agtaagcaac gccgcgctca aggcggttta cctgttgtag cccgcagtta tgggcaatat
    42121 cggtcgcttt cgaagaccag ggagagatgg tgccaggacg cggggtcacc agcaggagtt
    42181 tgccttgcgg ggcgtggctg gcgagtgccg gaccatattt cagcaggcgt tcaagttgtg
    42241 cgtgctcatc atcgtttaac ggcgcattga ggtcagcaaa atggacatac tcggcgtaaa
    42301 tattgtgaac cgggagcctg gcagcctgaa aacgtgccag cagtttgttg attcggaatg
    42361 ccgacagtgc aggcgaacca cgcagaattt ccatcataag tctctcgtct tctaagcttt
    42421 cggtgtaccc aagggggggg aaacgggcgt cattataaag aatctgatgc gctgacgaaa
    42481 ccgtttgcgt ggaaataaaa tcaccatcgt gaattagcaa cgcgtgccgc caatggctgt
    42541 aataagttgc catctggcgc aggtttacgc aaaatgccgc tcatttatga gtaaaccttt
    42601 cactattatt acgttttttc aagctgggac gcgcacgaca cagagaatta actaattgaa
    42661 aaaattaaag attaattatc tgttcatcgg cattctggca ctgctgctcg cggtcgctct
    42721 ctggccatcc attccctggt ttggtaaagc cgacaaccgt atcgccgcca ttcaagcgcg
    42781 gggagagttg cgtgtgagca ccattcatac tcccctgact tataacgaaa tcaacgggaa
    42841 accttttggc ctggattacg aactggcgaa acagtttgcc gattacctcg gcgtaaaact
    42901 gaaagtgacc gtgcggcaga atatcagcca gctgtttgac gaccttgata atggtaacgc
    42961 cgacctgctg gcggcaggac tggtctataa cagtgagcgg gtaaaaaatt atcagcctgg
    43021 ccctacctat tattccgtgt cacaacaact ggtttataaa gtgggtcagt atcgcccacg
    43081 tacgctgggc aacctgacgg cggagcaact caccgttgca ccgggtcatg tggtagttaa
    43141 cgatctccag accctgaaag aaacaaaatt cccggaatta agctggaagg tagacgacaa
    43201 aaaaggctct gcggaattaa tggaagatgt catcgaagga aaactcgatt acaccattgc
    43261 tgattctgtc gccatcagcc tgtttcagcg cgttcacccg gagctcgccg tagcgctcga
    43321 tatcaccgat gaacaaccgg tgacttggtt tagcccgtta gatggcgata ataccctttc
    43381 cgccgccctg ctcgacttct tcaacgaaat gaatgaagac ggtacgctgg cacgcattga
    43441 agagaaatac ctggggcatg gcgatgattt tgattacgtc gatacgcgca catttttacg
    43501 cgccgtcgat gcggtactgc cgcagttaaa gcccctgttt gagaaatacg ccgaagaaat
    43561 tgactggcgt ttgctggccg ctattgctta tcaggaatcg cactgggatg cacaggccac
    43621 ttcaccgacg ggtgtgcgcg gcatgatgat gttaaccaaa aataccgcgc aaagcctcgg
    43681 cattacggat cgtaccgatg ccgaacagag catcagcggt ggcgtgcgtt atttgcagga
    43741 tatgatgagt aaagtgccgg aaagtgtgcc ggagaacgaa cggatctggt ttgccctcgc
    43801 tgcgtacaat atgggctatg cgcatatgct ggatgcccgc gccctgacgg caaaaaccaa
    43861 agggaatcct gacagttggg ctgacgtaaa acagcgtctg cctttactta gccagaaacc
    43921 ctattacagc aagctgactt acggctacgc tcgtgggcat gaagcctacg cttatgtcga
    43981 aaatattcgt aagtatcaga ttagcctggt gggttatctg caagagaaag agaagcaggc
    44041 tacagaagcg gcgatgcaac tggcgcagga ttatccggcg gtatcgccta cggagttggg
    44101 caaagagaaa tttccttttc tctcgtttct ttcccagtcg tcatcaaact atttgaccca
    44161 ttctccctct ctgctgtttt ccaggaaagg gagtgaagag aaacaaaatt aatccgtcga
    44221 ggattgcgct tttttctgcg ctttaatttc ctggcggcgc atgcgaaaga agtcactgag
    44281 caacgccgcg cactcatccg ccagtattcc ttccgtaatt tccactcggt gattcatacc
    44341 cggatgatgc agcacatcca ttaaagatcc cgcagcgcca gttttcgcgt cacgcgcacc
    44401 aaagaccacg cgaccaatgc gactgtggat catcgctccg gcacacatta cacatggttc
    44461 aagcgtgaca tacaacgtgg cgtcgatcag acgataattt tgcatcacca gaccaccctg
    44521 ccgcagggcc atgatttctg catgtgcggt gggatcatgg cgaccaatcg ggcggttcca
    44581 gccttcgccg attacccgat tgttatgcac taataccgcg ccgaccggca cttcccgctc
    44641 atcccaggca cgtttcgcca gcgtcagcgc gtgacgcatc caatattcgt ggctaaattc
    44701 gacttcagac aaaaagaaaa ctccggttat aaaagcgcgg cgcattatac acggacgcta
    44761 tgctttactc tattccagtt gctggagttc accgcgcggg gttactcgcc aacgatgctg
    44821 acagaaataa agcagcggat tgtcctgatt actgtcgcta tagccactgt acagccgcag
    44881 cggcgtgccg attttgcgct ccagttgtgc gaccttttca tgtcccagac aacgcatcgt
    44941 caatacccaa ccaccatagc cacgctgaat ttggctggcg ataagattaa cccgcggcag
    45001 ccagggcgta tcgaaataaa ccgcttcaac cagcggctgc ggagagccgg taatcaacca
    45061 gatatcagca tcggaactta acaggtaggt ggttaatcgc tcctgaacca gcggaaaggc
    45121 ggtaacattg tcgcgaaacc agcgcacgaa atcggcctgc aacgtctgta aacgtgcttc
    45181 gctgtgacca aaagtgcacc cccacagaag cagactcatc ggccagcgtg ccgcacgacc
    45241 ttttatcaat aacgcaatgg ctataatcgg taacaacggc aggacaagta acgcattcag
    45301 cggttggcga cgcagtaaat agcgcagaaa actgccgaac atatcctgct gatgcaatgt
    45361 tccatctaag tcaaaaaaca ccacacgacg ctcgtgagtt gccaaaccat actcctctgg
    45421 actgaaacat cctgattaat tttcgctact acatagccta acagatagat catcactttt
    45481 ccggcaacaa tcgaccgcat catggctaac tggaattttt aattcatggc aattagcggc
    45541 aatggaatat aaaattcact cgcgtgtgtc tcatattgcc tgttgtcgcc actatttcat
    45601 cgcgaggaaa gatatgaacg gcttacttcg tatccgtcag cgttaccaag ggcttgccca
    45661 gagtgataaa aaactggcgg attatctgct gctacaacct gatacggcgc gccatttaag
    45721 ctctcagcaa ctggccaacg aagccggagt cagtcagtcc agcgtcgtga agttcgcgca
    45781 aaaactcggc tataaaggtt ttccggcgct taagttggcg ttgagtgaag cgctggcaag
    45841 ccagccggaa tcgccctccg tgcccattca taaccaaatc cgcggtgatg atccgttacg
    45901 gctggtcggc gaaaaactga ttaaagaaaa taccgccgct atgtacgcaa cgctaaacgt
    45961 taatagtgaa gagaaactgc atgaatgcgt aacaatgttg cgctctgcgc ggcggataat
    46021 tctgaccggt attggcgctt cgggtctggt ggcgcaaaac tttgcctgga agctgatgaa
    46081 gattggcttc aatgctgccg cactgcgcga tatgcatgcg ctactcgcaa cagtacaggc
    46141 gtcgtcccct gacgatctgt tattagccat ttcctacacc ggtgtacgac gcgagttaaa
    46201 cctggcggca gatgagatgc tgcgagtggg cggaaaagtg ctggcgatta ccggctttac
    46261 tccgaatgcc ctgcaacagc gtgcttctca ttgcctgtat accattgccg aagaacaagc
    46321 gacaaacagt gcttcaatct ctgcttgtca cgctcaggga atgttaacgg atttgctgtt
    46381 cattgcgctg attcagcagg atctggaact ggcaccagaa cgtattcgtc atagtgaagc
    46441 gctggtgaaa aaactggtct gagtaaagaa tgcgcgtata atgcccgccc ggtttgtgtt
    46501 gttttgagag tttccttatg gcgctgttaa tcactaaaaa atgcatcaat tgtgatatgt
    46561 gtgaaatcaa ataaaaacac atttattttc atagtgatac agcaatgtgt tattgaatgt
    46621 gttgttggat ttatggtgca aaaaaatttt gcataagttc agcaacacaa agaacaccac
    46681 tttctttaac gtcctccgcg ccctaaagga cggggcttta cggcgcaaaa ggcctgtgac
    46741 atacaacatt aacttttcag tcaaccgtag cacgttcttg tatacgccgt gtctgcggtt
    46801 ttatataacc ctggctggaa acctcttata caaagtcgat tcgccaacat catagatgat
    46861 cgccaccttc tggcgagaaa ctcctgatgc aattagtcgc ccggcctgcg cccattattc
    46921 tggtgaaagt ttgggacggc gaccgccaat tcgtccctgt gcgcgagcag cttccagtcc
    46981 tgcttttgtt cgttcaacaa tcagttcacg ttccatttca gccagggcac ccatcacatg
    47041 gaagaaaaag cgtcccattg gtgtgctggt atcaattgaa tcagtcagac tacgaaagtt
    47101 gatgcctcgt tcgcgcaacg cctccaccag cacgacaaga tgccgcatac tgcgccccag
    47161 ccgatccagc ttccagacaa ccagtgtgtc gcctgccgat aatgtcttga gcagtttttt
    47221 cagccccggt ctttcggact ttgtaccact tatcttgtct tcaaaaatca gctcgcatcc
    47281 tgcacagtt
//
LOCUS       DAJTRC010000023        45454 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.023, whole genome shotgun sequence.
ACCESSION   DAJTRC010000023 DAJTRC010000000
VERSION     DAJTRC010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 45454)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45454)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45454
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.023"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            95..2248
                     /locus_tag="OO357_001378"
     CDS             95..2248
                     /locus_tag="OO357_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SEL1-like repeat protein"
                     /protein_id="HCR5886379.1"
                     /translation="MYKSLFTIIILLYSASSYAKSESPPASTITFIAQIDHVELNKTA
                     LSHDMKLVLADRFYFKTSNPCETNSISARPESMDITDKAYADKLKKLETPFLDDRYFF
                     MTINQCDGNGTPMLTDIKVCTTELCGAEYTKKMSTVWLDETLKPTAKRLAIYFIQSPL
                     PYDEEKKQWKVTGWYISSTDNQSSTKQIAFEGYTDTDNLSDSKFISSFKSWFESGKIK
                     NIANYNPKGERDGKYESYYNSNGNVYESLSFSNGLINGEFITYYENGAIESKRHFNND
                     KIKDGECPHFYEDGKIKQQHSYLNSKLDGPAYEYYPDGKLMQENFYQQSVLIGNDTSY
                     YQSGKISSIHNRNSRGQYDGANERYSEEGKLLSKSVYKDGKQISVQTWYENGQKEEEK
                     HFDEQGQLNGLVKQWYKNGNLAKSQNYKHDILDGNSEEWYENGIPESLYPYKNGKTDG
                     IAKSWNKYGKLTYSIEYKNGVENGAYRNWSKNTGKLTKETLYVNGIRQGVEKEFNDRT
                     GKLLTSTQYVNNKRHGTEETYDQNGIKYITCYQNDQKLSSLDNPTQIKDNATTGDSSA
                     QFALGKYEFICANIDEGIKWLTKSAEQKNTDAIYFLATAYKGNGIPANNEKYLAYLQQ
                     AATLGSSNAQAEIGYLYLIGKELPQNLPDAGVWFKKAAAQGNFAAHFYLGRMYQNGDG
                     VERNMEKARFHLSNAAEGGIEPALKALNELEHQTK"
     gene            2301..4031
                     /locus_tag="OO357_001379"
     CDS             2301..4031
                     /locus_tag="OO357_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886380.1"
                     /translation="MKAKSTLLLMILSVFYSHQVLSSEPTQPDVYSVVEKKLNSALPL
                     EENTTFRSQAEWFNLQYELLVSGYPERAYNLLSKLETESKNAKYYLDLTASRVAEEEA
                     PETALTFLSKIGLNKPATLTSFESYINSWIKSKQPEKAMQLLSLDENALNYYLPTVLV
                     AYQIVPDKAISIYNSVYGDNVVIPYQQVTMLLTVAEGYQTKGDIKNAIHYADKALNMF
                     DKAIAVDSSRESHYSDEYLKLMNIYAANGNKEKAVVLSQRLHKAITESQSNYLSTLEG
                     ILLFYRNNDMQQDYQNSLSNYIVFIDKIFAFSPSTRSELSLINLLNSLNEVELMRKRL
                     AFLMTAPEYACYNSQYCYEDKVSALKILYRHHDNELMDKYFTTLLKEISNQPLEDWDI
                     IISNTGEKFSEVGLTKYAQHLASFAEEFYIKEQKSHSESEMRGLFSSLAELYSFGNDT
                     ASANRVFHQHVPAFNTDHMIQYFINAKEWNNARELLIKEEMLGMHNLILLENICMQKN
                     AECMAHITFTLNKLTTQPAITKIDSVGNEQLYQIGKIYHKLEIKPEPEQQVLIQSLYD
                     RASGSASATQ"
     gene            complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="OO357_001380"
     CDS             complement(4091..4582)
                     /gene="yajQ"
                     /locus_tag="OO357_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414960.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide binding protein YajQ"
                     /protein_id="HCR5886381.1"
                     /translation="MPSFDIVSEVDLQEARNAVDNASREVESRFDFRNVEASFELNDA
                     SKTIKVLSESDFQVNQLLDILRAKLLKRGIEGSSLDVPENIVHSGKTWFVEAKLKQGI
                     ESATQKKIVKMIKDSKLKVQAQIQGDEIRVTGKSRDDLQAVMAMVRGGDLGQPFQFKN
                     FRD"
     gene            4750..5661
                     /gene="panE"
                     /locus_tag="OO357_001381"
     CDS             4750..5661
                     /gene="panE"
                     /locus_tag="OO357_001381"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HCR5886382.1"
                     /translation="MKITVLGCGALGQLWLTALCKQGHEVQGWLRVPQPYCSVNLVET
                     DGSIFNESLTANDPDFLATSDLLLVTLKAWQVSDAVKSLASTLPVTTPILLIHNGMGT
                     IEELQNIQQPLLMGTTTHAARRDGNVIIHVANGITHIGPARQQDGDYSYLADILQTVL
                     PDVAWHNNIRAELWRKLAVNCVINPLTAIWNCPNGELRHHPQEIMQICEEVAAVIERE
                     GHHTSAEDLRDYVMQVIDATAENISSMLQDIRALRHTEIDYINGFLLRRARAHGIAVP
                     ENTRLFEMVKRKESEYERIGTGLPRPW"
     gene            5624..6214
                     /gene="yajL"
                     /locus_tag="OO357_001382"
     CDS             5624..6214
                     /gene="yajL"
                     /locus_tag="OO357_001382"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase YajL"
                     /protein_id="HCR5886383.1"
                     /translation="MSASALVCLAPGSEETEAVTTINLLVRGGIKVTTASVDSDGNLA
                     ITCSRGVKLLADAPLVEVADGEYDVIVLPGGIKGTECFRDSTLLVETVKQFHRSGRIV
                     AAICAAPATVLVPHDIFPIGNMTGFPTLKDKIPAEQWQDKRVVWDARVKLLTSQGPGT
                     AIDFGLKIIDLLVGREKAHEVASQLVMAAGIYNYYE"
     gene            complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="OO357_001383"
     CDS             complement(6268..7716)
                     /gene="thiI"
                     /locus_tag="OO357_001383"
                     /EC_number="2.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014168857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HCR5886384.1"
                     /translation="MKFIIKLFPEITIKSQSVRLRFIKILTGNIRNVLKHYDETLAVV
                     RHWDNIEVRAKDENQRLTIRDALTRIPGIHHILEVEDVPFTDMHDIFEKALVQYRDQL
                     EGKTFCVRVKRRGKHDFSSIDVERYVGGGLNQHIESARVKLTNPDVTVHLEVEDDRLL
                     LIKGRYEGIGGFPIGTQEDVLSLISGGFDSGVSSYMLMRRGCRVHYCFFNLGGAAHEI
                     GVRQVAHYLWNRFGSSHRVRFVAINFEPVVGEILEKIDDGQMGVILKRMMVRAASKVA
                     ERYGVQALVTGEALGQVSSQTLTNLRLIDNVSDTLILRPLISYDKEHIINLARQIGTE
                     DFARTMPEYCGVISKSPTVKAVKSKIEAEEEKFDFSILDKVVEEANNVDIREIAQQTE
                     QEVVEVETVNGFGPNDVILDIRSVDEQEDKPLKVEGIDVVSLPFYKLSTKFGDLDQNK
                     TWLLWCERGVMSRLQALYLREQGFNNVKVYRP"
     gene            7922..8164
                     /gene="xseB"
                     /locus_tag="OO357_001384"
     CDS             7922..8164
                     /gene="xseB"
                     /locus_tag="OO357_001384"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HCR5886385.1"
                     /translation="MPKKNEAPASFEKALSELEQIVTRLESGDLPLEEALNEFERGVQ
                     LARQGQAKLQQAEQRVQILLSDNEDASLTPFTPDNE"
     gene            8164..9063
                     /gene="ispA"
                     /locus_tag="OO357_001385"
     CDS             8164..9063
                     /gene="ispA"
                     /locus_tag="OO357_001385"
                     /EC_number="2.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2E,6E)-farnesyl diphosphate synthase"
                     /protein_id="HCR5886386.1"
                     /translation="MDFPQQLEACVKQANQALSRFIAPLPFQNTPVVETMQYGALLGG
                     KRLRPFLVYATGHMFGVSTNTLDAPAAAVECIHAYSLIHDDLPAMDDDDLRRGLPTCH
                     VKFGEANAILAGDALQTLAFSILSDANMPEVSDRDRISMISELASASGIAGMCGGQAL
                     DLDAEGKHVPLDALERIHRHKTGALIRAAVRLGALSAGDKGRRALPVLDKYAESIGLA
                     FQVQDDILDVVGDTATLGKRQGADQQLGKSTYPALLGLEQARKKARDLIDDARQALKQ
                     LAEQSLDTSALEALADYIIQRNK"
     gene            9088..10950
                     /gene="dxs"
                     /locus_tag="OO357_001386"
     CDS             9088..10950
                     /gene="dxs"
                     /locus_tag="OO357_001386"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HCR5886387.1"
                     /translation="MSFDIAKYPTLALVDSTQELRLLPKESLPKLCDELRRYLLDSVS
                     RSSGHFASGLGTVELTVALHYVYNTPFDQLIWDVGHQAYPHKILTGRRDKIGTIRQKG
                     GLHPFPWRGESEYDVLSVGHSSTSISAGIGIAVAAEKEGKNRRTVCVIGDGAITAGMA
                     FEAMNHAGDIRPDMLVVLNDNEMSISENVGALNNHLAQLLSGKLYSSLREGGKKVFSG
                     VPPIKELLKRTEEHIKGMVVPGTLFEELGFNYIGPVDGHDVLGLITTLKNMRDLKGPQ
                     FLHIMTKKGRGYEPAEKDPITFHAVPKFDPSSGCLPKSSGGLPSYSKIFGDWLCETAA
                     KDNKLMAITPAMREGSGMVEFSRKFPDRYFDVAIAEQHAVTFAAGLAIGGYKPIVAIY
                     STFLQRAYDQVLHDVAIQKLPVLFAIDRAGIVGADGQTHQGAFDLSYLRCIPEMVIMT
                     PSDENECRQMLYTGYHYNDGPSAVRYPRGNAVGVELTPLEKLPIGKGIVKRRGEKLVI
                     LNFGTLMPETAKVAESLNATLVDMRFVKPLDEALILEMAASHEALVTVEENAIMGGAG
                     SGVNEVLMAHRKPVPVLNIGLPDFFIPQGTQEEMRAELGLDAAGMEAKIKAWLA"
     gene            11005..11922
                     /gene="yajO"
                     /locus_tag="OO357_001387"
     CDS             11005..11922
                     /gene="yajO"
                     /locus_tag="OO357_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxyxylulose-5-phosphate synthase YajO"
                     /protein_id="HCR5886388.1"
                     /translation="MQYNPLGKTDLRVSRLCLGCMTFGEPDRGNHAWTLPEESSRPII
                     KRALEGGINFFDTANSYSDGSSEEIVGRALRDFARREDVVVATKVFHRVGDLPEGLSR
                     AQILRSINDSLRRLGMDYVDILQIHRWDYNTPIEETLEALNDVVKAGKARYIGASSMH
                     ASQFAQALELQKQHCWAQFVSMQDHYNLIYREEEREMLPLCYQEGVAVIPWSPLARGR
                     LTRPWGETTARLVSDEVGKNLYKESDENDAQIAERLTGVSEELGATRAQVALAWLLSK
                     PGIAAPIIGTSREEQLDELLNAVDITLKP"
     gene            complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="OO357_001388"
     CDS             complement(12033..12551)
                     /gene="pgpA"
                     /locus_tag="OO357_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706306.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HCR5886389.1"
                     /translation="MTILSRHKDVAKSRLKMSNPWHLLAVGFGSGLSPIVPGSMGSLA
                     AIPFWYLMTFLPWQLYSLVVMLGICIGVYLCHQTAKDMGVHDHGSIVWDEFIGMWITL
                     MALPTNDWQWVAAGFVIFRILDMWKPWPIRWFDRNVHGGMGIMIDDIVAGVISAGILY
                     FIGHHWPLGILS"
     gene            complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="OO357_001389"
     CDS             complement(12529..13506)
                     /gene="thiL"
                     /locus_tag="OO357_001389"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HCR5886390.1"
                     /translation="MACGEFSLIARYFDRVRSSRLDVELGIGDDCALLNIPEKQTLAI
                     STDTLVAGNHFLPDIDPADLAYKALAVNLSDLAAMGADPAWLTLALTLPDVDEAWLES
                     FSDSLFDLLNYYDMQLIGGDTTRGPLSMTLGIHGFVPMGRALTRSGAKPGDWIYVTGT
                     PGDSAAGLAILQNRLQVADAKDADYLIKRHLRPSPRILQGQALRDLANSAIDLSDGLI
                     SDLGHIVKASDCGARIDLALLPFSDALSRHVEPEQALRWALSGGEDYELCFTVPELNR
                     GALDVALGHLGVPFTCIGQMTADIEGLCFIRDGEPVTFDWKGYDHFVTP"
     gene            complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="OO357_001390"
     CDS             complement(13584..14003)
                     /gene="nusB"
                     /locus_tag="OO357_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HCR5886391.1"
                     /translation="MKPAARRRARECAVQALYSWQLSQNDIADVEYQFLAEQDVKDVD
                     VLYFRELLAGVATNTAYLDGLMKPYLSRLLEELGQVEKAVLRIALYELSKRSDVPYKV
                     AINEAIELAKSFGAEDSHKFVNGVLDKAAPVIRPNKK"
     gene            complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="OO357_001391"
     CDS             complement(14023..14493)
                     /gene="ribE"
                     /locus_tag="OO357_001391"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HCR5886392.1"
                     /translation="MNIIEANVATPDARVAITIARFNNFINDSLLEGAIDALKRIGQV
                     KDENITVVWVPGAYELPLAAGALAKTGKYDAVIALGTVIRGGTAHFEYVAGGASNGLA
                     HVAQDSEIPVAFGVLTTESIEQAIERAGTKAGNKGAEAALTALEMINVLKAIKA"
     gene            complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="OO357_001392"
     CDS             complement(14582..15685)
                     /gene="ribD"
                     /locus_tag="OO357_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HCR5886393.1"
                     /translation="MQDEYYMARALKLAQRGRFTTHPNPNVGCVIVKDGEIVGEGYHQ
                     RAGEPHAEVHALRMAGEKAKGATAYVTLEPCSHHGRTPPCCDALIAAGVARVVAAMQD
                     PNPQVAGRGLYRLQQAGIDVSHGLMMSEAEQLNKGFLKRMRTGFPYIQLKLGASLDGR
                     TAMASGESQWITSPQARRDVQRQRAQSHAILTSSATVLADDPALTVRWSELDEQTQAL
                     YPQQNLRQPVRIVIDSQNRVTPEHRIVQQPGETWFARTQEDSREWPETVRTLLIPEHK
                     GHLDLVVLMMQLGKQQINSIWVEAGPTLAGVLLQAGLVDELIVYIAPKLLGSDARGLC
                     SLPGLEKLADAPQFKFKEIRHVGPDVCLHLVGA"
     gene            complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="OO357_001393"
     CDS             complement(15689..16138)
                     /gene="nrdR"
                     /locus_tag="OO357_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HCR5886394.1"
                     /translation="MHCPFCFAVDTKVIDSRLVGEGSSVRRRRQCLVCNERFTTFEVA
                     ELVMPRVVKSNDVREPFNEEKLRSGMLRALEKRPVSSDYVEMAINHIKSQLRATGERE
                     VPSKMIGNLVMEQLKKLDKVAYIRFASVYRSFEDIKEFGEEIARLED"
     gene            16289..16828
                     /locus_tag="OO357_001394"
     CDS             16289..16828
                     /locus_tag="OO357_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5886395.1"
                     /translation="MNTNVFRLLLLGSLFSLSACVQQSEVRQMKHSVSTLNQEMTQLN
                     QETVKITQQNRLNAKSSSGVYLLPGAKTPARLESQIGTLRMSLVNITPDTDGTTLTLR
                     IQGESNDPLPAFSGTVEYGQIQGTIDNFQEINVQNQLINAPASVLAPSDVDIPLQLKG
                     ISVDQLGFVRIHDIQPVMQ"
     gene            17126..18010
                     /gene="tsx"
                     /locus_tag="OO357_001395"
     CDS             17126..18010
                     /gene="tsx"
                     /locus_tag="OO357_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-specific channel-forming protein Tsx"
                     /protein_id="HCR5886396.1"
                     /translation="MKKTLLAAGAVLALSSSFTVNAAENDKPQYLSDWWHQSVNVVGS
                     YHTRFGPQIRNDTYLEYEAFAKKDWFDFYGYADAPVFFGGNSDAKGIWNHGSPLFMEI
                     EPRFSIDKLTNTDLSFGPFKEWYFANNYIYDMGRNKDGRQSTWYMGLGTDIDTGLPMS
                     LSMNVYAKYQWQNYGAANENEWDGYRFKIKYFVPITDLWGGQLSYIGFTNFDWGSDLG
                     DDSGNAINGIKTRTNNSIASSHILALNYDHWHYSVVARYWHDGGQWNDDAELNFGNGN
                     FNVRSTGWGGYLVVGYNF"
     gene            complement(18187..18534)
                     /locus_tag="OO357_001396"
     CDS             complement(18187..18534)
                     /locus_tag="OO357_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH nuclease family protein"
                     /protein_id="HCR5886397.1"
                     /translation="MAIIPKNYARLESGYREKALKIYPWVCGRCSREFVYSNLRELTV
                     HHIDHDHTNNPEDGSNWELLCLYCHDHEHSKYTEADQYGTTVIAGEDAQKDVGEAKYN
                     PFADLKAMMNKKK"
     gene            complement(18663..19634)
                     /gene="secF"
                     /locus_tag="OO357_001397"
     CDS             complement(18663..19634)
                     /gene="secF"
                     /locus_tag="OO357_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HCR5886398.1"
                     /translation="MAQEYTVEQLNHGRKVYDFMRWDYWAFGISGLLLIAAIVIMGVR
                     GFNWGLDFTGGTVIEITLEKPAEIDVMRDALQKAGFEEPMLQNFGSSHDIMVRMPPAE
                     GETGGQVLGSQVLKVINESTNQNAAVKRIEFVGPSVGADLAQTGAMALMAALLSILVY
                     VGFRFEWRLAAGVVIALTHDVIITLGILSLFHIEIDLTIVASLMSVIGYSLNDSIVVS
                     DRIRENFRKIRRGTPYEIFNVSLTQTLHRTLITSGTTLMVILMLYLFGGPVLEGFSLT
                     MLIGVSIGTASSIYVASALALKLGMKREHMLQQKVEKEGADQPSILP"
     gene            complement(19645..21492)
                     /gene="secD"
                     /locus_tag="OO357_001398"
     CDS             complement(19645..21492)
                     /gene="secD"
                     /locus_tag="OO357_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HCR5886399.1"
                     /translation="MLNRYPLWKYVMLIVVIVIGLLYALPNLFGEDPAVQITGARGVA
                     ASEQTLIQVQKTLQEEKITAKSVALEEGAILARFDSTDTQLRAREALMGVMGDKYVVA
                     LNLAPATPRWLAAIHAEPMKLGLDLRGGVHFLMEVDMDTALGKLQEQNIDSLRSDLRE
                     KGIPYTTVRKENNYGLSITFRDAKARDEAIAYLSKRHPDLVISSQGSNQLRAVMSDAR
                     LSEAREYAVQQNINILRNRVNQLGVAEPVVQRQGADRIVVELPGIQDTARAKEILGAT
                     ATLEFRLVNTNVDQAAAASGRVPGDSEVKQTREGQPVVLYKRVILTGDHITDSTSSQD
                     EYNQPQVNISLDSAGGNIMSNFTKDNIGKPMATLFVEYKDSGKKDANGRAVLVKQEEV
                     INIANIQSRLGNSFRITGINNPNEARQLSLLLRAGALIAPIQIVEERTIGPTLGMQNI
                     EQGLEACLAGLLVSILFMIIFYKKFGLIATSALIANLILIVGIMSLLPGATLSMPGIA
                     GIVLTLAVAVDANVLINERIKEELSNGRTVQQAIDEGYRGAFSSIFDANITTLIKVII
                     LYAVGTGAIKGFAITTGIGVATSMFTAIVGTRAIVNLLYGGKRVKKLSI"
     gene            complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="OO357_001399"
     CDS             complement(21520..21852)
                     /gene="yajC"
                     /locus_tag="OO357_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000007628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HCR5886400.1"
                     /translation="MSFFISDAVAATGAPAQGSPMSLILMLVVFGLIFYFMILRPQQK
                     RTKEHKKLMDSIAKGDEVLTNGGLVGRVTKVAENGYIAIALNDTTEVVIKRDFVAAVL
                     PKGTMKAL"
     gene            complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="OO357_001400"
     CDS             complement(21875..23002)
                     /gene="tgt"
                     /locus_tag="OO357_001400"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HCR5886401.1"
                     /translation="MKFELDTTDGRARRGRLVFDRGVVETPCFMPVGTYGTVKGMTPE
                     EVEATGAQIILGNTFHLWLRPGQEIMKLHGDLHDFMQWKGPILTDSGGFQVFSLGDIR
                     KITEQGVHFRNPINGDPIFLDPEKSMEIQYDLGSDIVMIFDECTPYPADWDYAKRSME
                     MSLRWAKRSRERFDSLGNKNALFGIIQGSIYEDLRDISVKGLVDIGFDGYAVGGLAVG
                     EPKADMHRILEHVCPQIPADKPRYLMGVGKPEDLVEGVRRGIDMFDCVMPTRNARNGH
                     LFVTDGVVKIRNAKYKSDTGPLDPECDCYTCRNYSRAYLHHLDRCNEILGARLNTIHN
                     LRYYQRLMAGLRKAIEEGKLESFVTDFYQRQGREVPPLNVD"
     gene            complement(23057..24127)
                     /gene="queA"
                     /locus_tag="OO357_001401"
     CDS             complement(23057..24127)
                     /gene="queA"
                     /locus_tag="OO357_001401"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HCR5886402.1"
                     /translation="MRVTDFSFELPESLIAHYPMPERSSCRLLSLDGPTGALTHGTFT
                     DLLDKLNPGDLLVFNNTRVIPARLFGRKASGGKIEVLVERMLDDKRILAHIRASKAPK
                     PGAELLLGDDESINATMTARHGALFEVEFNDERSVLDILNSIGHMPLPPYIDRPDEDA
                     DRELYQTVYSEKPGAVAAPTAGLHFDEPLLEKLRAKGVEMAFVTLHVGAGTFQPVRVD
                     TIEDHIMHSEYAEVPQDVVDAVLAAKARGNRVIAVGTTSVRSLESAAQAAKNDLIEPF
                     FDDTQIFIYPGFQYKVVDALVTNFHLPESTLIMLVSAFAGYQHTMNAYKAAVEEKYRF
                     FSYGDAMFITYNPQAINERVGE"
     gene            24220..24793
                     /gene="acpH"
                     /locus_tag="OO357_001402"
                     /pseudo
     CDS             24220..24793
                     /gene="acpH"
                     /locus_tag="OO357_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414938.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP phosphodiesterase"
     gene            complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="OO357_001403"
     CDS             complement(24798..26615)
                     /gene="malZ"
                     /locus_tag="OO357_001403"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin glucosidase"
                     /protein_id="HCR5886403.1"
                     /translation="MMLNAWHLPVPPFVKQSKDQLLITLWLTGEDPPQRIMLRTEHDN
                     EEMSVPMHKQRSQPQPGVTAWRAAIDLSSGQPRRRYSFKLLWHDRQRWFTPQGFSRMP
                     PARLEQFAVDVPDIGPQWAADQIFYQIFPDRFARSLPREAEQDHVYYHHAAGQEIILR
                     DWDEPVTAQAGGSTFYGGDLDGISEKLPYLKKLGVTALYLNPVFKAPSVHKYDTEDYR
                     HVDPQFGGDGALLRLRHNTQQLGMRLVLDGVFNHSGDSHAWFDRHNLGTGGACHNSES
                     PWRDWYSFSDDGTALDWLGYASLTKLDYQSESLVNEIYRGEDSIVRHWLKAPWNMDGW
                     RLDVVHMLGEAGGARNNMQHVAGITEAAKETQPEAYIVGEHFGDARQWLQADVEDAAM
                     NYRGFTFPLWGFLANTDISYDPQQIDAQTCMAWMDNYRAGLSHQQQLRMFNQLDSHDT
                     ARFKTLLGRDIARLPLAVVWLFTWPGVPCIYYGDEVGLDGKNDPFCRKPFPWQVEKQD
                     TALFALYRRMIVLRKKSQALRRGGCQVLYAEDNVVVFVRVLNQQRVLVAINRGEASEV
                     VLPASPFLNAVQWQCKEGHGQLTDGILALPAISATVWMN"
     gene            complement(26771..28144)
                     /gene="proY"
                     /locus_tag="OO357_001404"
     CDS             complement(26771..28144)
                     /gene="proY"
                     /locus_tag="OO357_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline-specific permease ProY"
                     /protein_id="HCR5886404.1"
                     /translation="MESKNKLKRGLSTRHIRFMALGSAIGTGLFYGSADAIKMAGPSV
                     LLAYIIGGIAAYIIMRALGEMSVHNPAASSFSRYAQENLGPLAGYITGWTYCFEILIV
                     AIADVTAFGIYMGVLFPTVPHWIWVLSVVLIICAVNLMSVKVFGELEFWFSFFKVATI
                     IIMIVAGFGIIIWGIGNGGQPTGIHNLWSNGGFFSNGWLGMVMSLQMVMFAYGGIEII
                     GITAGEAKDPEKSIPRAINSVPMRILVFYVGTLFVIMSIYPWNQVGTAGSPFVLTFQH
                     MGITFAASILNFVVLTASLSAINSDVFGVGRMLHGMAEQGSAPKIFSKTSRRGIPWVT
                     VLVMTTALLFAVYLNYIMPENVFLVIASLATFATVWVWIMILLSQIAFRRRLPPEEVK
                     ALKFKVPGGVATTIGGLIFLLFIIGLIGYHPDTRISLYVGFAWIVVLLIGWMFKRRHD
                     RQLAENQ"
     gene            complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="OO357_001405"
     CDS             complement(28220..29539)
                     /gene="brnQ"
                     /locus_tag="OO357_001405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transporter carrier
                     protein BrnQ"
                     /protein_id="HCR5886405.1"
                     /translation="MTHQLRSRDIIALGFMTFALFVGAGNIIFPPMVGLQAGEHVWTA
                     AFGFLITAVGLPVLTVVALAKVGGGVDSLSTPIGKVAGVLLATVCYLAVGPLFATPRT
                     ATVSFEVGIAPLTGDSALPLFIYSLVYFAIVILVSLYPGKLLDTVGNFLAPLKIIALV
                     ILSVVAIVWPAGSISTATEAYQNAAFSNGFVNGYLTMDTLGAMVFGIVIVNAARSRGV
                     TEARLLTRYTVWAGLMAGVGLTLLYLALFCLGSDSASLVDQSANGAAILHAYVQHTFG
                     GGGSFLLAALIFIACLVTAVGLTCACAEFFAQYVPLSYRTLVFILGGFSMVVSNLGLS
                     QLIQISVPVLTAIYPPCIALVVLSFTRSWWHNSSRVIAPPMFISLLFGILDGIKASAF
                     SDILPSWAQRLPLAEQGLAWLMPTVVMVVLAIIWDRAAGRQVTSSAH"
     gene            complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="OO357_001406"
     CDS             complement(29946..31241)
                     /gene="phoR"
                     /locus_tag="OO357_001406"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate regulon sensor histidine kinase PhoR"
                     /protein_id="HCR5886406.1"
                     /translation="MLERLSWKRLVLELLLCCLPAFILGAFFGYLPWFLLASVTGLLI
                     WHFWNLLRLSWWLWVDRSMTPPPGRGSWEPLLYGLHQMQLRNKKRRRELGNLIKRFRS
                     GAESLPDAVVLTTEEGGIFWCNGLAQQILGLRWPEDNGQNILNLLRYPEFTQYLKTRD
                     FSRPLNLVLNTGRHLEIRVMPYTHKQLLMVARDVTQMHQLEGARRNFFANVSHELRTP
                     LTVLQGYLEMMDEQPLEGAVREKALHTMREQTQRMEGLVKQLLTLSKIEAAPTQLLNE
                     KVDVPMMLRVVEREAQTLSQKKQTFTFEIDNGLKVSGNEDQLRSAISNLVYNAVNHTP
                     EGTHITVRWQRVPHGAEFSVEDNGPGIAPEHIPRLTERFYRVDKARSRQTGGSGLGLA
                     IVKHAVNHHESRLNIESTVGKGTRFSFVIPERLIAKNSD"
     gene            complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="OO357_001407"
     CDS             complement(31299..31988)
                     /gene="phoB"
                     /locus_tag="OO357_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000113934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate response regulator transcription
                     factor PhoB"
                     /protein_id="HCR5886407.1"
                     /translation="MARRILVVEDEAPIREMVCFVLEQNGFQPVEAEDYDSAVNQLNE
                     PWPDLILLDWMLPGGSGIQFIKHLKRESMTRDIPVVMLTARGEEEDRVRGLETGADDY
                     ITKPFSPKELVARIKAVMRRISPMAVEEVIKMQGLSLNPTSHRVMAGEEPLEMGPTEF
                     KLLHFFMTHPERVYSREQLLNHVWGTNVYVEDRTVDVHIRRLRKALEPGGHDRMVQTV
                     RGTGYRFSTRF"
     gene            32178..33380
                     /gene="sbcD"
                     /locus_tag="OO357_001408"
     CDS             32178..33380
                     /gene="sbcD"
                     /locus_tag="OO357_001408"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcD"
                     /protein_id="HCR5886408.1"
                     /translation="MRILHTSDWHLGQNFYSKSREAEHQAFLDWLLETAQTHQVDAII
                     VAGDVFDTGSPPSYARTLYNRFVVNLQQTGCHLVVLAGNHDSVATLNESRDIMAFLNT
                     TVVASAGHAPQILPRRDGTPGAVLCPIPFLRPRDIISSQAGLNGIEKQQHLLAAITDY
                     YQQHYADACKLRGDQPLPIIATGHLTTVGSSKSDAVRDIYIGTLDAFPAQNFPPADYI
                     ALGHIHRAQIIGGMEHVRYCGSPIPLSFDECGKSKYVHLVTFSNGKLESVENLNVPVT
                     QPMAVLKGDLASITAQLEQWRDVSQEPPVWLDIEITTDEYLHDIQRKIQALTESLPVE
                     VLLVRRSREQRERVLASQQRETLSELSVEEVFNRRLALEDLDESQQQRLQHLFTTTLR
                     TLAGEHEA"
     gene            33377..36520
                     /gene="sbcC"
                     /locus_tag="OO357_001409"
     CDS             33377..36520
                     /gene="sbcC"
                     /locus_tag="OO357_001409"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease subunit SbcC"
                     /protein_id="HCR5886409.1"
                     /translation="MKILSLRLKNLNSLKGEWKIDFTREPFASNGLFAITGPTGAGKT
                     TLLDAICLALYHETPRLSNVSQSQNDLMTRDTAECLAEVEFEVKGEAYRAFWSQNRAR
                     NQPDGNLQVPRVELARCADGKILADKVKDKLELTATLTGLDYGRFTRSMLLSQGQFAA
                     FLNAKPKERAELLEELTGTEIYGQISAMVFEQHKSARTELEKLQAQASGVTLLTPEQV
                     QSLTASLQVLTDEEKQLITAQQQEQQSLNWLTRQDELQQEASRRQQALQQALAEEEKA
                     QPQLAALSLAQPARNLRPHWERIAEHSAALAHIRQQIEEVNTRLQSTMALRASIRHHA
                     AKQSAELQQQQQSLNTWLQEHDRFRQWNNELAGWRAQFSQQTSDREHLRQWQQQLTHA
                     EQKLNALAAITLTLTADEVATALAQHAEQRPLRQRLVALHGQIVPQQKRLAQLMVTIQ
                     NVTLEQTQRNVALNEMRQRYKEKTQQLADVKTICEQEARIKTLEAQRAQLQAGQPCPL
                     CGSTSHPAVEAYQALEPGVNQSRLLALENEVKKLGEEGAALRGQLDALTKQLQRDENE
                     AQSLRQDEQALTQQWQAVTASLNITLQPQDDIQPWLDAQDEHERQLRLLSQRHELQGQ
                     IAAHNQQIIQYQQQIEQRQQQLLTALAGYALTLPQEDEEESWLATRQQEAQSWQQRQN
                     ELTALQNRIQQLTPILETLPQSDDLPHSEETVALDNWRQVHEQCLALHSQQQTLQQQD
                     VLAAQSLQKAQAQFDTALQASVFDDQQAFLAALMDEQTLTQLEQLKQNLENQRRQAQT
                     LVTQTAETLAQHQQHRPDGLALTVTVEQIQQELAQTHQKLRENTTSQGEIRQQLKQDA
                     DNRQQQQTLMQQIAQMTQQVEDWGYLNSLIGSKEGDKFRKFAQGLTLDNLVHLANQQL
                     TRLHVRYLLQRKASEALEVEVVDTWQADAVRDTRTLSGGESFLVSLALALALSDLVSH
                     KTRIDSLFLDEGFGTLDSETLDTALDALDALNASGKTIGVISHVEAMKERIPVQIKVK
                     KINGLGYSKLESTFAVK"
     gene            36646..37827
                     /gene="araJ"
                     /locus_tag="OO357_001410"
     CDS             36646..37827
                     /gene="araJ"
                     /locus_tag="OO357_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter AraJ"
                     /protein_id="HCR5886410.1"
                     /translation="MKKVILSLALGTFGLGMAEFGIMSVLTELAHNVGISIPAAGHMI
                     SYYALVVVVGAPIIALFSSRYSLKHILLFLVALCVIGNAMFTLSSSYLMLAIGRLVSG
                     FPHGAFFGVGAIVLSKIIKPGKVTAAVAGMVSGMTVANLLGIPLGTYLSQECWRYTFL
                     LIAVFNIAVMASVYFWVPDIRDEAKGNLREQFHFLRSPAPWLIFAATMFGNAGVFAWF
                     SYVKPYMMFISGFSETAMTFIMMLVGLGMVLGNMLSGRISGRYSPLRIAAVTDFIIVL
                     ALLMLFFCGGMKTTSLIFAFICCAGLFALSAPLQILLLQNAKGGELLGAAGGQIAFNL
                     GSAVGAYCGGMMLTLGLAYNYVALPAALLSFAAMSSLLLYGRYKRQQAADSPVLAKPL
                     G"
     gene            complement(37970..38878)
                     /gene="mak"
                     /locus_tag="OO357_001411"
     CDS             complement(37970..38878)
                     /gene="mak"
                     /locus_tag="OO357_001411"
                     /EC_number="2.7.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414928.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructokinase"
                     /protein_id="HCR5886411.1"
                     /translation="MRIGIDLGGTKTEVIALGDAGEQLYRHRLPTPRDDYRQTIETIA
                     TLVDMAEQATGQRGTVGMGIPGSISPYTGVVKNVNSTWLNGQPFDKDLSARLQREVRL
                     ANDANCLAVSEAVDGAAAGAQTVFAVIIGTGCGAGVAFNGRTHIGGNGTAGEWGHNPL
                     PWMDEDELRYREEVPCYCGKQGCIETFISGTGFATDYRRLSGHALKGSEIIRLVEESD
                     PVAELALRRYELRLAKSLAHVVNILDPDVIVLGGGMSNVDRLYQTVGQLIKQFVFGGE
                     CETPVSKAKHGDSSGVRGAAWLWPQE"
     gene            39003..39914
                     /gene="rdgC"
                     /locus_tag="OO357_001412"
     CDS             39003..39914
                     /gene="rdgC"
                     /locus_tag="OO357_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination-associated protein RdgC"
                     /protein_id="HCR5886412.1"
                     /translation="MLWFKNLMVYRLSREISLRAEEMEKQLASMAFTPCGSQDMAKMG
                     WVPPMGSHSDALTHVANGQIVICARKEEKILPSPVIKQALEAKIAKLEAEQARKLKKT
                     EKDSLKDEVLHSLLPRAFSRFSQTMMWLDTVNGLIMVDCASAKKAEDTLALLRKSLGS
                     LPVVPLSMENPIELTLTEWVRSGSAAQGFQLLDEAELKSLLEDGGVIRAKKQDLTSEE
                     ITNHIEAGKVVTKLALDWQQRIQFVMCDDGSLKRLKFCDELRDQNEDIDREDFAQRFD
                     ADFILMTGELAALIQNLIEGLGGEAQR"
     gene            complement(39992..40447)
                     /locus_tag="OO357_001413"
     CDS             complement(39992..40447)
                     /locus_tag="OO357_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886413.1"
                     /translation="MTQRPWSKLQREIYDLLTPTINLQIHCTRYPMRSQNGGSTDLPR
                     YWITLDKNVIWDYPKDFIAGNAGVRNFHGETCWYPYLTDICSISDLLREYIDTPKAEL
                     LTKQFTSDKWGLVNILRAADRRIGMRRLDQLRRKTHNIAALKIIARRSE"
     gene            complement(40444..40779)
                     /locus_tag="OO357_001414"
                     /pseudo
     CDS             complement(40444..40779)
                     /locus_tag="OO357_001414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308469.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing protein"
     gene            complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="OO357_001415"
     CDS             complement(40934..41218)
                     /gene="ppnP"
                     /locus_tag="OO357_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine/purine nucleoside phosphorylase"
                     /protein_id="HCR5886414.1"
                     /translation="MLQSNEYFSGKVKSIGFSSSSTGRASVGVMVEGEYTFSTAEPEE
                     MTVISGALNVLLPDATDWQVYEAGSVFNVPGHSEFHLQVAEPTSYLCRYL"
     gene            complement(41290..41967)
                     /locus_tag="OO357_001416"
     CDS             complement(41290..41967)
                     /locus_tag="OO357_001416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AroM family protein"
                     /protein_id="HCR5886415.1"
                     /translation="MSASLAILTIGIVPMQEVLPLLTEYIDEDNISHHSLLGKLSREE
                     VMAEYAPEAGEDTILTLLNDNQLAHVSRRKVERDLQGVVEVLDNQGYDVILLMSTANI
                     SSMTARNTIFLEPSRILPPLVSSIVEDHQVGVIVPVEEMLPVQAQKWQILQKSPVFSL
                     GNPIHDSEQKIIDAGKELLAKGADVIMLDCLGFHQRHRDLLQKQLDVPVLLSNVLIAR
                     LAAELLV"
     gene            complement(42225..42416)
                     /gene="yaiA"
                     /locus_tag="OO357_001417"
     CDS             complement(42225..42416)
                     /gene="yaiA"
                     /locus_tag="OO357_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YaiA"
                     /protein_id="HCR5886416.1"
                     /translation="MPTKPPYPREAYIVTIEKGKPGQTVTWYQLRADHPKPDSLISEH
                     PTAQEAMDAKKRYEDPDKE"
     gene            complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="OO357_001418"
     CDS             complement(42466..42990)
                     /gene="aroL"
                     /locus_tag="OO357_001418"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase AroL"
                     /protein_id="HCR5886417.1"
                     /translation="MTQPLFLIGPRGCGKTTVGMALADSLNRRFVDTDQWLQSQLNMT
                     VAEIVEREEWAGFRARETAALEAVTAASTVIATGGGIILTEFNRHFMQNNGIVVYLCA
                     PVSVLVNRLQAAPEEDLRPTLTGKPLSEEVQEVLEERDALYREVAHIIIDATNEPSQV
                     ISEIRSALAQTINC"
     gene            complement(43173..43631)
                     /locus_tag="OO357_001419"
     CDS             complement(43173..43631)
                     /locus_tag="OO357_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706275.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HCR5886418.1"
                     /translation="MTIWVDADACPNVIKEILYRAAERMQMPLVLVANQSLRVPPSRF
                     IRTLRVAAGFDVADNEIVRQCEAGDLVITADIPLAAEAIEKGAAALNSRGERYTPATI
                     RERLTMRDFMDTLRASGIQTGGPDSLSQRDRQAFAAELEKWWLEVQRSRG"
     gene            43751..44560
                     /gene="proC"
                     /locus_tag="OO357_001420"
     CDS             43751..44560
                     /gene="proC"
                     /locus_tag="OO357_001420"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HCR5886419.1"
                     /translation="MEKKIGFIGCGNMGKAILGGLIASGQVLPGQIWVYTPSPDKVAA
                     LHDQFGINAAESAQEVAQIADIIFAAVKPGIMIKVLSEITSSLNKDSLVVSIAAGVTL
                     DQLARALGHDRKIIRAMPNTPALVNAGMTSVTPNALVTPEDTADVLNIFRCFGEAEVI
                     AEPMIHPVVGVSGSSPAYVFMFIEAMADAAVLGGMPRAQAYKFAAQAVMGSAKMVLET
                     GEHPGALKDMVCSPGGTTIEAVRVLEEKGFRAAVIEAMTKCMEKSEKLSKS"
     gene            complement(44577..>45454)
                     /gene="adrA"
                     /locus_tag="OO357_001421"
                     /gene_synonym="dgcC"
     CDS             complement(44577..>45454)
                     /gene="adrA"
                     /locus_tag="OO357_001421"
                     /gene_synonym="dgcC"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="diguanylate cyclase AdrA"
                     /protein_id="HCR5886420.1"
                     /translation="PHLAWQIASRAVDPLSREIYNLKTDAVLAGMWVGVMGVNVLPST
                     AMLMILCLNLMGAGGPRLFVAGLVLMVVSCLVTLELTGITVSFNSAPLEWWLSLPIIV
                     VYPLMFGWVSYQTATKLAEHKRRLQVMSTRDGMTGVYNRRHWETMLRNEFDNCRRHNR
                     DATLLIIDIDHFKSINDTWGHDVGDEAIVALTRQLQITLRGSDVIGRFGGDEFAVIMS
                     GTPAESAITAMLRVREGLNTLRLPNTPQVTLRISVGVAPLNPQMSHYREWLKSADLAL
                     YKAKKAGRNRTEVAA"
BASE COUNT        11813 a        11854 c        11115 g        10672 t
ORIGIN      
        1 tcgcttaatt ccatataact ggacgattac attagaagta aactatactt tttatgaaat
       61 atcagggata aattattcac atggaaaata catcatgtac aaatcattat ttactattat
      121 cattctactg tatagcgcca gttcatatgc aaaaagcgaa tcccctcccg caagcactat
      181 aactttcatt gcacaaatcg atcatgttga gctaaataaa acagcattat ctcatgatat
      241 gaaactcgtt cttgccgatc gtttttattt caaaaccagt aatccttgcg aaacaaattc
      301 aatttctgct cgaccagaat caatggatat tactgacaaa gcctatgccg acaaattaaa
      361 aaaattagaa actccttttc ttgatgatcg ctatttcttt atgaccatca atcagtgtga
      421 cggtaatggc actcccatgc tgacagacat aaaagtatgt acaacagaat tatgtggtgc
      481 tgaatataca aagaaaatgt ctactgtatg gctcgatgaa actttaaaac caactgctaa
      541 acgtctggca atttatttta ttcaatctcc tttaccctat gacgaggaga aaaaacaatg
      601 gaaagtcacc ggatggtata tctcatcaac tgataatcag tcttcaacta aacagattgc
      661 atttgaagga tatacagaca cagataacct ctccgattct aaatttattt cttcatttaa
      721 atcctggttt gaaagtggaa agataaaaaa cattgctaac tataatccaa aaggagaaag
      781 agatggaaaa tacgaaagtt attacaatag caatggtaac gtctatgaat ctctaagttt
      841 ttctaatggt cttatcaacg gtgagtttat tacatattat gaaaatgggg ccattgaaag
      901 taaacgtcat tttaataacg acaaaattaa agatggtgaa tgccctcatt tttatgaaga
      961 tgggaaaatc aaacagcaac atagctatct gaatagtaaa ctcgatggtc ctgcatatga
     1021 atattatcct gatggcaaac tcatgcagga aaatttctat cagcaaagtg tgttgattgg
     1081 aaacgatacc agttactacc aatccggaaa aataagctct atacacaata gaaattcccg
     1141 tggtcagtat gatggagcta acgaacgtta tagtgaagag ggtaagttac tgtctaaatc
     1201 cgtctataaa gacggaaaac aaatttctgt tcaaacctgg tatgaaaatg ggcagaaaga
     1261 agaggagaaa cattttgatg agcaaggcca attaaatggt ctggttaagc aatggtataa
     1321 aaatggtaat cttgcaaaat cacaaaacta taaacatgac atactcgatg gtaacagtga
     1381 agagtggtac gaaaatggca tccctgaaag tttgtaccct tataagaatg gaaaaaccga
     1441 cggcattgcc aaatcatgga ataagtatgg taaattaacc tattcaatcg aatacaaaaa
     1501 tggcgtagag aatggggcat atcgtaactg gagcaaaaac acaggtaaac taaccaaaga
     1561 aaccctgtac gtcaatggta tcagacaggg agtggaaaaa gagtttaacg atcgcacagg
     1621 taaactatta acctccacgc aatatgttaa taataaaaga catggtacgg aggaaactta
     1681 tgatcaaaac ggaataaaat atattacatg ctatcaaaat gatcagaaat tatcgtcttt
     1741 agacaatcca acacaaataa aagataatgc aacaacaggc gattccagtg ctcaatttgc
     1801 attagggaaa tacgaattca tctgtgcaaa tatagatgaa gggattaaat ggttaaccaa
     1861 atctgcagag caaaaaaaca ctgatgcgat atacttttta gctacagcct acaaaggcaa
     1921 tggtatacct gcaaacaatg aaaaatacct agcatatctg caacaagctg caacactggg
     1981 cagtagtaat gcccaggcgg aaattggcta tttatatctc atcggaaaag aactaccaca
     2041 gaatcttcct gatgctggtg tctggttcaa aaaagcagct gcacaaggta attttgccgc
     2101 acatttctat ctgggaagga tgtatcagaa tggtgacggc gttgagagaa atatggaaaa
     2161 agccaggttt catctatcta atgcggcaga aggtggaata gaacctgcgc ttaaagcatt
     2221 aaatgaactt gaacatcaaa caaaataaag cgctcaggga ccacgtaagg ttcatcaaac
     2281 agaaaaggat atagtttgcg atgaaagcga aatccacttt gttattaatg attttatctg
     2341 ttttttattc tcatcaggtt ttatccagcg aacccactca gccggatgtt tattctgtcg
     2401 tcgagaaaaa actaaactct gctttacctc tggaagaaaa taccaccttc agatcgcagg
     2461 ctgaatggtt taatttgcaa tatgaattat tagtatctgg ctaccctgaa cgtgcttaca
     2521 atttactgag caaacttgag acagaatcga aaaacgctaa atattatctg gatttaactg
     2581 cctccagagt cgctgaagaa gaagcaccag agacagcctt aactttttta tcaaaaattg
     2641 ggttaaataa accggctact cttacatcct ttgagagcta tatcaatagc tggataaaaa
     2701 gcaaacaacc cgaaaaggct atgcagcttc tcagcctcga tgagaatgcc ctgaattatt
     2761 atttaccgac tgtgttagtc gcatatcaga tcgttcctga taaggcaata agtatttata
     2821 attctgtata tggagataat gttgttatac cctatcagca agtcaccatg ttacttacag
     2881 ttgcggaggg ttatcaaaca aaaggcgata taaaaaacgc cattcattat gccgataagg
     2941 cgctgaatat gtttgataaa gctattgctg tagatagcag tcgtgaatcc cattatagtg
     3001 atgagtattt gaaactcatg aatatctacg ccgccaatgg caacaaagaa aaagcggtgg
     3061 ttttatctca acgtctacac aaagccatta ccgaatctca atcgaattac ttaagtactc
     3121 tggaaggaat tttattgttt tatagaaata atgatatgca gcaagactat caaaacagcc
     3181 tttcaaatta catagtattt atcgataaaa tttttgcctt ttccccatca acaagaagtg
     3241 aattaagcct cattaattta ttaaattcat taaatgaggt ggaattgatg agaaaacgtc
     3301 tggcgtttct gatgacagca ccagaatacg cttgttataa tagtcagtat tgttatgaag
     3361 acaaagtcag cgcattaaaa atcctgtatc gtcatcatga caatgagcta atggacaaat
     3421 atttcacgac tcttcttaaa gagatcagta atcaaccatt agaagactgg gatataataa
     3481 taagtaatac tggtgaaaaa tttagtgaag tgggtctaac caaatatgct caacatctcg
     3541 catctttcgc tgaagaattt tatataaaag agcaaaaatc acattctgaa agtgaaatga
     3601 gaggactatt ttcatcactt gccgaattat atagttttgg taatgatact gccagcgcaa
     3661 accgagtgtt ccatcagcat gtccctgcct tcaatacaga tcatatgatt caatatttta
     3721 taaatgccaa agaatggaac aatgccaggg aattattgat aaaagaggaa atgttgggta
     3781 tgcataatct gatattgttg gagaatattt gtatgcaaaa aaatgcagaa tgcatggcac
     3841 acattacctt tacgcttaat aaattaacaa cacaaccagc aataacaaaa atagatagcg
     3901 tgggtaatga gcaactttat cagattggca aaatctacca taagttggaa attaaacctg
     3961 agccagagca acaggtttta attcagtcgc tttatgaccg ggcatcagga agcgcctctg
     4021 caactcaata aatgcatggt caaaagggcc accacggtgg ccctgacaat cagtagctat
     4081 atccagaaag ttaatcgcgg aagtttttga actggaacgg ctgaccgaga tcgccaccgc
     4141 gcaccatcgc catgacagcc tgcaaatcat cacgagattt gccagtcacg cggatctcat
     4201 cgccctgaat ttgcgcctgc actttcagtt tgctgtcttt gatcatcttg acgatttttt
     4261 tctgagtggc gctctcaatg ccctgtttca gtttcgcttc cacaaaccag gttttaccgc
     4321 tatgaacgat attttccggt acatccagtg aactgccttc aatgccgcgc ttcagcagct
     4381 tggcacgcag aatatccagc aactgattga cctggaagtc ggactcgctc aacactttga
     4441 tggttttgct ggcgtcgttc agctcaaatg aggcttcaac gttacggaag tcaaaacggg
     4501 actccacttc gcgactcgcg ttatcgaccg cgttacgtgc ttcctgaaga tcaacttcag
     4561 agacaatatc gaaagatggc atcttttcct ctcccttcat ttttgatgcg aagcataata
     4621 cctgcaaagt taaataacca acagtcctgc accaggacgt atcccttaat tgtgcatagt
     4681 cgcaacagca tgcacaatta agggatagcc ttattgaaca cccggagtgg ttgcgggtga
     4741 ggaggaacaa tgaaaattac cgtattggga tgcggtgcct tagggcaatt atggcttaca
     4801 gcactttgta aacagggtca tgaagttcag ggctggctgc gtgtaccgca accttattgt
     4861 agtgtgaatc tggttgagac agatggttcg atatttaacg aatcgctgac cgccaacgat
     4921 cccgattttc tcgccaccag cgatctgctc ctggtgacgc tgaaagcatg gcaggtttcc
     4981 gatgccgtca aaagcctcgc atccacactg cctgtaacta cgccaatact gttaattcac
     5041 aacggcatgg gcaccatcga agaattgcaa aacattcagc agccattact gatgggcacc
     5101 accacccatg ccgcccgccg cgacggcaat gtcattattc atgtggcaaa cggaatcacg
     5161 catatcggtc cggcacggca acaggatggc gattacagtt atctggcgga tattttgcaa
     5221 accgtgttgc ctgacgttgc ctggcataac aatattcgcg ccgagctgtg gcgcaagctg
     5281 gccgtcaact gcgtgattaa tccactgact gccatctgga attgcccgaa cggtgaatta
     5341 cgtcatcatc cgcaagaaat tatgcaaata tgcgaagaag tcgcggcagt gatcgaacgc
     5401 gaagggcatc atacttcagc ggaagatttg cgtgattacg tgatgcaggt gattgatgcc
     5461 acagcagaaa atatctcgtc gatgttgcag gatatccgcg cgctgcgcca caccgaaatc
     5521 gactatatca atggttttct cttacgccgc gcccgcgcgc atgggattgc cgtaccggaa
     5581 aacacccgcc tgtttgaaat ggtaaaaaga aaggagagtg aatatgagcg catcggcact
     5641 ggtttgcctc gcccctggta gtgaagagac tgaagccgtc accacgatca acctcttggt
     5701 tcgcggcggt atcaaagtca ccactgccag cgtcgacagc gatggcaacc tggcaattac
     5761 ctgctcgcgc ggcgtgaagc tgctggcgga tgcgccgctg gtcgaagtgg ctgatggcga
     5821 atatgacgtg atcgtgctgc ctggtggcat taaaggcacg gagtgttttc gcgatagcac
     5881 tctgctggtt gaaaccgtta aacagttcca ccgttccggg cgtatcgtcg cggctatttg
     5941 cgccgcgcca gccaccgtgc tggtgccgca cgatatcttt ccgattggca atatgaccgg
     6001 cttcccgacg ctgaaagaca aaattcccgc tgaacaatgg caggacaagc gcgtcgtctg
     6061 ggatgcacgg gtaaaattgc tgaccagcca ggggccgggt acagctatcg actttggtct
     6121 gaaaattatc gacctgctgg ttgggcgcga gaaagcccat gaagtggcat cacaactggt
     6181 gatggcggca gggatttata attattacga gtagtgtcgg atgcggcaaa cgtcgcatct
     6241 gaccagatgc gacgtaaaaa ctacaaatta cgggcgatat accttcacat tgttaaagcc
     6301 ttgctcgcgc agatagagcg cctgcagacg gctcatcacc ccgcgctcac accacagcag
     6361 ccaggttttg ttctggtcga gatcgccaaa tttggtgctc agtttataga acggcagaga
     6421 aaccacatca atcccttcga ctttcagtgg cttatcttcc tgttcatcga cagaacggat
     6481 atcgagaatc acgtcgttcg ggccgaagcc gttgacggtt tccacttcca ccacttcctg
     6541 ctcggtctgc tgggcgattt cgcggatatc aacgttattc gcttcctcaa ccactttatc
     6601 gagaatactg aagtcgaact tctcttcttc cgcttcaatc ttcgatttaa ccgctttcac
     6661 cgtcgggctt ttggagatca cgccacaata ttccggcatc gtgcgagcaa agtcttcggt
     6721 gccaatctgg cgagccaggt tgatgatgtg ctctttgtcg taagaaatca gcggacgcag
     6781 gatcagcgtg tcagagacgt tatcaatcag gcgcaggttg gtcagcgtct gactggacac
     6841 ctggccgagc gcttcgccgg tgaccagcgc ctgtacgccg taacgttcag ccactttaga
     6901 tgcggcacgc accatcatac gtttgaggat aacgcccatc tgaccgtcgt cgattttctc
     6961 gagaatttcc ccgacaaccg gttcgaaatt aatggcgaca aaacgcacgc ggtgggagct
     7021 gccaaagcgg ttccacagat aatgcgccac ctgacgcacg ccaatttcat gcgctgcgcc
     7081 gccgaggtta aagaagcagt aatgcacgcg gcagccgcga cgcatcaaca tataactgga
     7141 aacaccggag tcgaaaccac cggaaatgag cgacagcaca tcttcctggg tgccaatcgg
     7201 gaaaccacca ataccttcgt agcgaccttt aatcagcagg aggcgatcgt cttccacttc
     7261 cagatgaaca gttacatccg ggttagtcag cttcacgcgc gcggattcaa tatgctgatt
     7321 taaaccgccg ccgacgtaac gttccacatc aatcgagcta aaatcatgtt tgccacggcg
     7381 cttcacgcgt acgcagaagg ttttgccttc cagctgatcg cgatactgaa ccaacgcttt
     7441 ctcaaaaata tcgtgcatgt cggtaaacgg cacgtcttcg acttcgagaa tatggtggat
     7501 acccggaata cgggtcaggg cgtcgcgaat agtcagacgc tggttttcat ctttcgcgcg
     7561 aacttcgatg ttatcccagt ggcggacgac agcgagcgtc tcatcatagt gctttaaaac
     7621 gttacgaatg ttcccggtaa ggatttttat aaagcgcaag cgcacagatt ggcttttgat
     7681 ggtgatttcc gggaacaatt taatgataaa cttcatggcg gcaatggttc gttggcaagc
     7741 cttaagcgac ttgtataggg aaaaatacag cagcccacac ctgcggctgc atccaggcgc
     7801 ggaagtatac cactaacatc gctttgctgc gcacatcacc ttaccattgc gcgttatttg
     7861 ctatttgccc tgagtccgtt accatgacgg ggcgaaaaat attgagagtc agacattcat
     7921 tatgccgaag aaaaatgagg cgcccgccag ctttgaaaag gcgctgagcg agctggaaca
     7981 gattgtaacc cgtctggaaa gtggcgacct gccgctggaa gaggcgctga acgagttcga
     8041 acgcggcgtg cagctggcac gtcaggggca ggccaaatta caacaagccg aacagcgcgt
     8101 acaaattctg ctgtctgaca atgaagacgc ctctctaacc ccttttacac cggacaatga
     8161 gtaatggact ttccgcagca actcgaagcc tgcgttaagc aggccaacca ggcgctgagc
     8221 cgttttatcg ccccactgcc ctttcagaac actcccgtgg tcgaaaccat gcagtatggc
     8281 gcattattag gtggtaagcg cctgcgacct ttcctggttt atgccaccgg tcatatgttt
     8341 ggcgttagca caaacacgct ggacgcaccc gctgctgccg tagagtgtat ccacgcttac
     8401 tcattaattc atgatgattt accggcgatg gatgatgacg atctgcgtcg cggtttgccg
     8461 acctgccatg tgaagtttgg cgaagcaaac gcgattctcg ctggcgacgc tttacaaacg
     8521 ctggcgttct cgattctaag cgatgccaat atgccggaag tgtcggatcg cgacagaatt
     8581 tcgatgattt ctgaactggc gagcgccagc ggtattgccg gaatgtgcgg tggtcaggca
     8641 ttagatttag acgcggaagg caaacacgta cctctggacg cgcttgagcg tattcatcgt
     8701 cataaaaccg gcgcattgat tcgcgccgcc gttcgccttg gtgcattaag cgccggagat
     8761 aaagggcgtc gtgctctgcc agtactcgac aagtacgcag agagcatcgg ccttgccttc
     8821 caggttcagg atgacatcct ggatgtggta ggagatactg caacgttggg aaaacgccag
     8881 ggtgccgacc agcaacttgg taaaagtacc taccctgcac ttctgggtct tgagcaagcc
     8941 cggaagaaag cccgggatct gatcgacgat gcccgtcagg cgctgaaaca actggctgaa
     9001 cagtcactcg atacctcggc actggaagcg ctagcggact acatcatcca gcgtaataaa
     9061 taaacaataa gtattaatag gcccctgatg agttttgata ttgccaaata cccgaccctg
     9121 gcactggtcg actccaccca ggagttacga ctgttgccga aagagagttt accgaaactc
     9181 tgcgacgaac tgcgccgcta tttactcgac agcgtgagcc gttccagcgg gcacttcgcc
     9241 tccgggctgg gcacggtcga actgaccgtg gcgctgcact atgtctacaa caccccattt
     9301 gaccaattga tttgggatgt gggacatcag gcttatccgc ataaaatttt gaccggacgc
     9361 cgcgacaaaa tcggcaccat ccgtcagaaa ggcggtctgc acccgttccc gtggcgcggc
     9421 gaaagcgaat atgacgtatt aagcgtcggg cattcatcaa cctccatcag tgccggaatt
     9481 ggtattgcgg ttgctgccga gaaagaaggc aaaaatcgcc gcaccgtctg tgtgattggc
     9541 gacggcgcga ttaccgctgg catggcgttt gaagcgatga atcacgcggg cgatattcgt
     9601 cctgatatgc tggtggtcct caacgacaat gaaatgtcga tttccgaaaa tgttggcgcg
     9661 cttaacaacc atctggctca gctgctttcc ggtaagcttt actcttcact gcgcgaaggc
     9721 gggaaaaaag ttttctctgg cgtgccgccc attaaagagc tgctcaaacg taccgaagaa
     9781 catattaaag gcatggtggt gcctggcacg ttgtttgaag agctgggatt taactacatc
     9841 ggcccggtgg acggtcacga tgtgctgggg cttatcacca cgctaaagaa catgcgcgac
     9901 ctgaaaggcc cgcagttcct gcatatcatg accaaaaaag gtcgtggtta tgaaccggca
     9961 gaaaaagacc cgatcacttt ccacgccgtg cctaaatttg atccctccag cggttgtttg
    10021 ccgaaaagta gcggcggttt gccgagctat tcaaaaatct ttggcgactg gttgtgcgaa
    10081 acggcagcga aagacaacaa gctgatggcg attactccgg cgatgcgtga aggttccggc
    10141 atggtcgagt tttcacgtaa attcccggat cgttacttcg acgtggctat tgccgagcaa
    10201 cacgcagtga cctttgccgc cggtctggcg attggtggat acaaacccat tgtcgcgatc
    10261 tactccacct tcctgcaacg cgcctatgat caggtgctgc atgacgtggc aattcaaaag
    10321 ctaccggtcc tgttcgccat tgaccgcgcg ggcattgttg gtgctgacgg tcaaactcac
    10381 cagggcgctt ttgatctctc ttacctgcgc tgcataccag aaatggtcat tatgaccccg
    10441 agcgatgaaa acgaatgtcg ccagatgctc tataccggct atcactataa cgatggcccg
    10501 tccgcggtgc gctacccgcg cggcaacgcg gttggcgtgg aactgacgcc gctggaaaaa
    10561 ctaccaattg gcaaaggcat tgtgaagcgt cgtggcgaga aactggtgat ccttaacttt
    10621 ggtacgctga tgccagaaac ggccaaagtc gccgaatcgc tgaacgctac gctggtcgat
    10681 atgcgttttg tgaaaccact tgatgaagcg ttaattctgg aaatggccgc cagccatgaa
    10741 gcgctggtca ccgtagaaga aaacgccatt atgggcggcg caggtagcgg agtgaacgaa
    10801 gtgctgatgg cccatcgtaa accagtaccc gtgctgaaca ttggcctgcc ggacttcttt
    10861 attccgcaag gaactcagga agaaatgcgc gccgaactcg gcctcgatgc cgccggtatg
    10921 gaagccaaaa tcaaggcctg gctggcataa tccctactcc actcctgcta tgcttaagaa
    10981 attattcatg gcaggagtgg cagcatgcaa tacaacccct taggaaaaac cgaccttcgc
    11041 gtttcccgac tttgcctcgg ctgtatgacc tttggcgagc cagatcgcgg taatcacgca
    11101 tggacactgc cggaagagag cagccgtccc ataattaaac gcgcgctgga aggtggcata
    11161 aatttctttg ataccgccaa tagctattcc gatggtagca gcgaagagat cgttggtcgc
    11221 gcactgcggg atttcgcccg tcgtgaagac gtagtcgttg ccaccaaagt gttccatcgc
    11281 gttggtgatt taccggaagg attatcccgt gcacaaattt tgcgctctat caacgacagc
    11341 ctgcgacgtc tcggcatgga ttatgtcgat atcctgcaaa ttcatcgctg ggattacaac
    11401 acgccgatcg aagagacgct ggaagccctg aacgacgtgg taaaagccgg gaaagcgcgt
    11461 tatatcggcg cgtcatccat gcacgcttcg cagtttgctc aggcgctgga actacaaaaa
    11521 cagcactgct gggcgcagtt tgtcagtatg caggatcact ataatctgat ttatcgcgaa
    11581 gaagagcgcg agatgctgcc tctgtgttat caggagggcg tggcggtgat tccgtggagc
    11641 ccgctggcgc gggggcgact gacgcgtcca tggggagaaa ctaccgcacg actggtgtct
    11701 gatgaggtgg ggaaaaatct ctataaagaa agcgatgaaa atgacgcgca gatcgcagag
    11761 cggttaacgg gcgtcagtga agaactcggt gcaacacgag cacaagttgc gctggcctgg
    11821 ttgttaagta aaccgggcat tgccgcaccg attatcggaa cttcgcggga agaacagctt
    11881 gatgagctgc tgaacgcggt ggatatcact ttgaagccat aacagattgc cgagctggaa
    11941 acgccgtata aaccgcatgc ggtcgtagga tttaaataag agtccaggcc tgatgagacg
    12001 tgacaagcgt cacatcaggc atcggtgcac aactacgaca gaatacccag cggccagtga
    12061 tgaccgataa agtacaggat gcctgcggaa atcaccccgg cgacaatatc gtcgatcatg
    12121 atccccatgc cgccatgtac attgcgatca aaccagcgga tcggccacgg cttccacata
    12181 tccagaatac ggaaaatcac aaacccggcg gcaacccact gccagtcatt ggtcggcagc
    12241 gccatgagcg tgatccacat accaataaat tcgtcccaga caatgctgcc gtgatcgtgc
    12301 acgcccatat ctttcgccgt ttgatgacaa agatagacgc cgatacagat ccccaacatc
    12361 accaccagcg agtagagctg ccagggtaaa aaggtcatca gataccagaa agggatcgcc
    12421 gccagcgatc ccatcgagcc aggaacgatt gggcttaatc cacttccgaa tccgacagca
    12481 agcagatgcc acggattact catcttcagg cgacttttcg cgacatcttt atggcgtgac
    12541 aaaatggtca tatcctttcc agtcaaacgt gacaggttcg ccgtcacgaa taaaacaaag
    12601 cccttcgata tcggcggtca tttgcccgat acaggtaaac ggtacgccaa ggtgtccgag
    12661 agcaacatcc agcgcgccac ggttcagttc cggcaccgtg aaacacaact cgtaatcttc
    12721 accgccagag agcgcccagc gcagcgcctg ttccggttca acatggcgag aaagcgcatc
    12781 agaaaacggc agcaatgcca ggtcaatacg tgcgccgcag tcgctggctt tcacgatatg
    12841 cccgagatcg gaaatcagac cgtcagagag atcgatagct gaatttgcca gatcgcgcag
    12901 tgcctgcccc tgtaaaatac gcggcgatgg acggagatga cgtttgatca agtagtcagc
    12961 atctttagca tcggcaacct gcaaacggtt ttgcaaaatc gccagcccgg cggcgctatc
    13021 gcccggtgta ccggtcacat agatccagtc acccggtttc gccccagagc gcgttaaggc
    13081 tcgtcccatc ggaacaaagc cgtggatacc caacgtcatt gataatggcc cacgcgtggt
    13141 atcgccgcca atgagttgca tatcgtaata attgagaaga tcaaacaaac tgtcgctgaa
    13201 ggactcaagc cacgcttcgt ctacgtccgg taaggttaat gccagcgtca gccaggccgg
    13261 atcggccccc atcgctgcca gatcgcttag gttcaccgcc agtgctttat aagccagatc
    13321 agcaggatcg atatcaggga ggaaatggtt gcccgccacc agcgtatcag tgctgatcgc
    13381 cagggtctgt ttctcgggga tattgagaag agcgcaatcg tcgccgatgc ccagttcgac
    13441 atcaagacga gaacttctta cacggtcaaa ataacgggca atcagggaga actcgccaca
    13501 tgccatacgt tatgcctcag caggtaaaaa agaaaaggcc ggcgatcatg gaacagtctt
    13561 ccgtgaatct accggcctgg atattacttt ttgttagggc gaatcacagg tgctgcttta
    13621 tcgagtacgc cgttgacgaa cttatggctg tcttctgcgc cgaacgattt cgccagttcg
    13681 atcgcttcgt taatggccac tttgtatggc acatcactac gtttagacag ttcgtacagc
    13741 gcaatgcgca gtactgcttt ttctacctgg cccagttctt ccagcaggcg ggacaggtag
    13801 ggcttcatca gtccgtcgag gtatgcggtg ttagtcgcca ccccggccag cagctcacgg
    13861 aagtacagga cgtcaacgtc ttttacatcc tgttcagcca ggaactggta ttcaacatca
    13921 gcgatgtcgt tctgggacaa ctgccaggag tagagcgcct ggacggcaca ctcacgagcg
    13981 cggcgacgag cagcaggttt cacggatttc cccttactaa tttcaggcct tgatggcttt
    14041 caatacatta atcatttcaa gcgcggtcag tgcagcttct gcacctttgt tgccagcttt
    14101 ggtgccagca cgttcgatcg cttgttcaat gctttcagtg gtcagaaccc caaaagcaac
    14161 cggaatttcg ctgtcctggg caacatgcgc caggccgttg cttgcaccac cagcgacata
    14221 ttcaaagtgg gcagtgccac cacgaataac cgtacccagc gcaatcaccg cgtcgtattt
    14281 accggtttta gccagtgcac ccgccgccag cggcagctca taggcaccag gcacccaaac
    14341 aacggtaatg ttttcatctt ttacctgacc gatacgtttc agtgcgtcaa ttgcaccttc
    14401 cagcaggctg tcattgataa agttgttgaa acgcgcgatg gtgatggcga cgcgagcgtc
    14461 cggggtagca acgttagctt caataatgtt cattttcttc cttcgggttc gagtatggcc
    14521 ccgcaggggg gcggatttta gcataatatt tcgtgcgctg cttccctttc gagccgggag
    14581 atcatgcacc cactaaatgc aggcaaacat ccgggcctac atgacgtatc tctttgaatt
    14641 taaattgggg ggcgtcggct aatttctcaa gccctggcag cgagcataat ccacgggcgt
    14701 cgctgcctaa tagtttaggt gcgatataga caatcagctc atcgactaaa cccgcctgta
    14761 gcaatacgcc agcgagcgtt ggccccgctt ccacccagat gctgttaatc tgctgtttac
    14821 ccagttgcat catcagtaca accagatcca gatgaccttt atgctctgga atcagcaagg
    14881 tacgcaccgt ttccggccac tcacgagaat cttcctgcgt acgcgcgaac caggtttcgc
    14941 cgggctgctg cacaatgcga tgttccggcg tcacgcgatt ttggctatca atcacaatac
    15001 gtactggctg gcggagattt tgttgcgggt agagcgcctg agtttgttca tccagttcag
    15061 accaacgcac tgttaaggct ggatcatccg ccagcaccgt ggcgctgctg gttaaaatgg
    15121 catgactttg tgcgcgctgt cgttgtacgt cgcgccgcgc ctgaggcgaa gtgatccact
    15181 ggctttcgcc gctcgccatc gccgtgcggc catcaagcga tgcgccaagt tttaactgaa
    15241 tataaggaaa gccggtgcgc atccgcttga gaaagccttt attcaattgc tcggcttcac
    15301 tcatcatcag cccgtggctg acgtcaatgc cagcctgttg cagacggtaa agtccacgcc
    15361 cagcgacctg cgggttagga tcttgcatcg ctgcgaccac gcgcgctacg ccagcggcga
    15421 tgagtgcgtc acagcacggt ggcgtacggc catgatggct acagggttcg agtgtcacat
    15481 acgccgttgc gcctttggct ttttcacccg ccatacgcaa cgcgtgtact tcggcatgtg
    15541 gttcacccgc tcgttggtgg taaccttcac cgacaatttc gccatctttg acaatgacgc
    15601 acccgacatt cgggttgggg tgggtggtaa aacgtcctcg ttgcgccagc tttagcgccc
    15661 gcgccatgta atactcgtcc tgcacggctt agtcctccag gcgcgcgatc tcttcgccaa
    15721 attctttgat atcttcgaaa ctgcggtaga cagaggcaaa acggatatag gcgactttat
    15781 cgagcttttt caattgctcc atcaccagat tgccaatcat cttgctcggt acttcgcgct
    15841 caccggtggc gcgcagctgc gatttaatat gattgattgc catttcaacg taatcggaac
    15901 tcaccggacg tttttccagc gcccgcagca ttccgctacg caatttctct tcattaaacg
    15961 gttcacgcac gtcgttgctt tttacaacac gcggcataac cagctccgcc acttcaaagg
    16021 tggtgaaacg ttcattacac accagacact gccgacggcg gcgtacggat gaaccctcgc
    16081 ccacgagacg agagtcaatt accttagtgt ccacggcgaa acagaatggg caatgcatac
    16141 ggcgtcctga ccaggtggtt aacggaaatc tattttaccc tgaactgacg atacaacaaa
    16201 ggcgcaacgc ttttgagata aaggatcgat gctttcgctg cttttaccct ctaccattag
    16261 gctaattgcg acgagaaagg gacgacctat gaatactaac gtttttcgac tgctcctgct
    16321 gggaagcctg ttcagcctta gcgcctgtgt gcagcaaagt gaagtgcgac agatgaaaca
    16381 cagcgtcagc acgctgaacc aggagatgac gcaactcaat caagaaacag tcaaaatcac
    16441 tcagcaaaac aggctgaatg caaaatccag cagtggggtt taccttctgc ctggagcgaa
    16501 aacaccggca agactggaaa gccagatcgg tactttacgt atgtcgctgg tgaatattac
    16561 gcctgataca gatggcacca cccttacact acgtattcag ggtgagtcga atgatccctt
    16621 gcccgcattc agcggaactg ttgaatatgg gcaaattcag ggaacaatag acaactttca
    16681 ggaaatcaat gtgcagaatc aattgattaa tgcccctgcc agcgtcctcg cccccagcga
    16741 tgttgatatt ccgttacaat taaaggggat ttctgtggat cagttaggct ttgtacgtat
    16801 ccacgacatt caacctgtta tgcagtaaac gtatttcggg acgattttgt gcgtcccgca
    16861 acatcttccc cgtcattttg ttactctgct tacatcacct ggattgatag taaaagtttg
    16921 caacaagggc gaaagtcagt acaatccccg cccgaatgtg tgtaaacgtg aacgcaatcg
    16981 attacgtaaa tgatagaact gtgaaacgaa acatattttt gtgagcaatg atttttataa
    17041 taggctcctc tgtatacgaa atatttagaa acgcaatttg cgcctttttc actcccgcaa
    17101 gggattttca aacagtggca tacatatgaa aaaaacatta ctggcagccg gtgcggtact
    17161 ggcgctctct tcgtctttta ctgtcaacgc agctgaaaac gacaaaccgc agtatctttc
    17221 cgactggtgg caccagagcg ttaacgttgt cggaagctat cacacccgtt tcggaccgca
    17281 gatccgcaac gatacctacc ttgagtacga agcattcgct aaaaaagact ggttcgactt
    17341 ctatggttat gcggatgcgc cggtattctt cggcggtaac tccgatgcta aaggtatctg
    17401 gaaccacggt tctccgctgt ttatggaaat cgaaccacgt ttctccatcg acaagctgac
    17461 caatactgac cttagcttcg gtccgttcaa agagtggtac ttcgcgaaca actacattta
    17521 cgacatgggt cgtaataaag atggtcgcca gagcacctgg tacatgggtc tgggtaccga
    17581 tatcgacact ggcctgccga tgagcctgtc catgaacgtc tatgcgaaat accagtggca
    17641 gaactatggc gcagcgaacg aaaacgagtg ggacggttac cgtttcaaaa ttaaatactt
    17701 tgtgccgatt accgatctgt ggggcggtca gctgagctac atcggcttca ccaacttcga
    17761 ctggggttcc gatttagggg atgacagcgg taacgcaatc aacggtatta agacccgtac
    17821 taataactct atcgcttcca gccatattct ggctctgaac tacgatcact ggcactactc
    17881 tgtcgtagct cgttactggc acgacggtgg tcagtggaac gacgatgcag aactgaactt
    17941 cggcaacggc aacttcaacg ttcgctctac cggctggggt ggttacctgg tagtaggtta
    18001 caacttctga ttatgaaaat gccgggattt attcccggca tttctgattg ttaacaacgt
    18061 gtgctttgtt catgccggat gcggcgtaaa cgctttgtcc ggcccacaaa agcacgtaaa
    18121 ttcaatatat tgctgaaata acgtaggcct gataagcgta gcgcatcagg caattttacg
    18181 ttttaatcac ttcttcttgt tcatcatcgc tttcaggtca gcgaatgggt tgtacttcgc
    18241 ttcaccgaca tctttctgcg catcttcccc tgcgataacg gtcgtaccat actgatccgc
    18301 ttcggtatat ttcgaatgct catgatcgtg gcaatagaga cacaacaatt cccagttact
    18361 gccatcttcc gggttattgg tatggtcgtg atcaatgtgg tgaacggtaa gttcacgcag
    18421 gttagaataa acaaactcgc gggaacagcg accgcagacc cacggataga tttttaatgc
    18481 tttttcgcga tagccgcttt ctaaccgtgc atagtttttt gggatgatag ccatgaaaag
    18541 ttacctggat gaaatgtagg gttacgcgca gtttcccaag atgcggggat tttcgcaatc
    18601 tccatacatc aaaaaaaatc ccgatcttct gaccgggatt ttcaacatca acgggaactt
    18661 gattaaggca gaattgacgg ctgatccgcc ccttcttttt ccactttctg ctgcaacatg
    18721 tgttcgcgct tcatacccag tttcagagcc aacgcagatg ctacgtagat ggaagatgca
    18781 gtgccgatgg aaacaccgat aagcatggtc agcgagaagc cttccagtac cggaccaccg
    18841 aagagataca gcatcaggat aaccatcaag gtagtaccgg atgtgatcaa ggtacggtgc
    18901 agcgtctggg tcaaggacac gttaaagatt tcgtaaggcg taccgcgacg gatcttgcgg
    18961 aagttttcac gaattcggtc cgataccacg atactgtcgt taagcgagta accgataacc
    19021 gacatcaacg atgccacaat ggtcaggtca atctcgatat ggaataacga caaaataccc
    19081 agcgtaatga taacgtcgtg cgtcagcgca ataaccaccc ctgccgccag tcgccactca
    19141 aagcggaaac ctacgtacac gaggatagac agcagcgctg ccatcaacgc catcgcaccg
    19201 gtttgcgcaa ggtcagcccc cacgctcgga ccgacgaact caatacgctt cactgctgca
    19261 ttctgattgg tggattcgtt aatcaccttc agaacttggc tgcccagcac ctgaccgccg
    19321 gtttcgcctt cagcaggcgg catacggacc atgatgtcgt ggctgctacc aaagttttgc
    19381 agcattggct cttcaaaacc ggctttttgc aatgcatcac gcattacgtc aatttcagcc
    19441 ggtttttcga gcgtaatttc gataaccgta ccaccggtga aatccagccc ccagttaaag
    19501 ccgcgcacgc ccataataac gatagcagcg attaacagca gaccagagat gccgaaagcc
    19561 cagtagtccc agcgcataaa gtcatagact ttacggccgt ggtttagttg ttcaacagta
    19621 tattcctgtg ccacatcgca ctcctcagat tgacagcttc ttgacgcgct tgccgccata
    19681 tagcaggttt acgatggcac gcgtaccgac aatcgcggta aacatcgacg tcgccacacc
    19741 gataccggta gtaatcgcga accctttaat tgccccggta cccactgcgt acaggatgat
    19801 gactttaatc agcgtggtga tgttcgcatc gaagatagaa ctgaatgcgc cacgataacc
    19861 ttcatcaatt gcctgttgaa cagtacgtcc gttgctcaac tcttctttaa tacgttcgtt
    19921 gatcagtacg ttcgcatcga ccgctaccgc aagggttaag acgatacccg cgatacctgg
    19981 catactcagt gttgcgcctg gcaacagcga cataataccg acgatcaaga tcaagttggc
    20041 aatcagagca ctggtcgcaa tcagaccaaa cttcttatag aagatgatca tgaacagaat
    20101 agaaaccagc agaccggcaa ggcaagcctc cagcccctgc tcaatgttct gcatacccag
    20161 ggtcgggcca attgtacgtt cttcaacaat ctgaatcggc gcgatcaacg caccggcacg
    20221 cagcagcagt gacagctgac gggcttcgtt cgggttgttg atgccggtga tacggaagct
    20281 gttacccaga cgagactgga tgttggcgat gttaatcacc tcttcctgtt tcaccagaac
    20341 cgcacgacca tttgcatctt tcttaccgct gtctttgtac tccacaaaca gggttgccat
    20401 cggtttgccg atgttgtcct tggtgaagtt agacataatg ttgccaccag cgctatcgag
    20461 cgagatgtta acctgcggct ggttgtattc gtcctggctg gaagtggagt cggtgatatg
    20521 gtcaccagtc agaattacgc gtttgtacag cacaaccggc tgaccttcgc gggtctgttt
    20581 cacttcagag tcgcccggta cgcgaccgga tgccgcagcg gcctgatcaa cattggtgtt
    20641 taccagacgg aattccagcg ttgccgtcgc gcccagaatc tctttcgcac gcgcagtgtc
    20701 ctgaataccg ggcagttcaa caacgatacg gtcagcaccc tgacgctgaa ccaccggctc
    20761 cgccacgcca agttggttta cacggttacg caggatatta atgttctgct gcaccgcata
    20821 ttcacgcgct tcactcagac gagcatcgct cattaccgca cgcagctggt tgctgccctg
    20881 gctgctaatc accaggtccg gatggcgctt gctcagatac gcaatggctt catcacgagc
    20941 tttagcatcg cggaaagtga tgctcaggcc gtagttgttt tctttacgaa cagtggtata
    21001 cgggatgccc ttttcgcgca ggtcactgcg taggctatcg atattttgtt cctggagttt
    21061 gccaagcgcg gtgtccatat ccacttccat caggaagtga acgccgccac gcaggtcaag
    21121 gccgagcttc atcggctcag catgaatagc tgccagccag cgcggcgttg ccggggcaag
    21181 gttaagcgcc acgacgtatt tgtcacccat aacgcccatt aatgcttcac gagcgcgcaa
    21241 ctgggtgtca gtggagtcga agcgcgcaag aatagcgccc tcttccagtg ccacagactt
    21301 agcagttatt ttttcttctt gtaacgtttt ctggacctgg atcagcgttt gctcactggc
    21361 ggcgactccg cgcgcaccag tgatctgaac agccggatcc tcaccaaaca ggttgggaag
    21421 cgcatacagc agaccgatga caatcaccac gatcagcatg acgtacttcc acaaaggata
    21481 acggtttaac acggcaattc ccttagggaa aaattttaat tacagcgcct tcatggtgcc
    21541 tttcggcagg acggcagcta cgaagtcacg tttaataact acttcagtgg tgtcattcag
    21601 cgcgatagca atgtagccgt tttccgctac tttggttacg cgaccaacca ggccaccgtt
    21661 cgtcagaact tcatcacctt tggcaatgga gtccatcagc tttttgtgtt ctttggtgcg
    21721 cttctgctgt ggacgcagga tcatgaaata gaaaatcaga ccgaatacca ccagcatcaa
    21781 aatcaaagac atcgggctac cttgcgccgg tgcacccgtt gccgctaccg catcagaaat
    21841 aaaaaagctc attaaatttc cctcattatt aatattaatc aacgttcaaa ggtggtactt
    21901 ctcgcccctg acgctggtaa aaatcagtta cgaagctctc taatttaccc tcttcaatag
    21961 ccttgcgtaa acccgccatc aaacgctggt agtaacgaag gttatgaatg gtgttgagtc
    22021 gcgcgcctaa tatttcgttg caacggtcga gatgatgcaa gtaagcgcgt gaataattgc
    22081 gacaggtgta gcaatcacac tcaggatcga gtgggccagt atcgctctta tatttcgcat
    22141 tgcggatttt caccacgcca tcggtcacga acaaatgacc atttcgggcg ttgcgggttg
    22201 gcattacgca gtcaaacata tcgataccgc gacgtacgcc ttcaaccagg tcttccggtt
    22261 taccaacgcc catcaggtaa cgcggtttgt ctgccggaat ttgcggacac acatgctcca
    22321 gaatacggtg catatctgct ttcggctcac ccacagccag accgccgaca gcgtagccat
    22381 caaagccgat atctaccaga cctttaacag atatatcacg taaatcttcg taaatactgc
    22441 cctgaattat accaaataac gcatttttgt taccaagact gtcaaaacgc tcacggctac
    22501 gcttcgccca acgcagagac atctccatag agcgttttgc gtaatcccag tcagcaggat
    22561 acggcgtaca ctcatcaaag atcatgacga tatccgaacc aagatcgtac tgaatctcca
    22621 tcgacttttc cgggtcgagg aaaatcgggt cgccgttgat cgggttacgg aagtgaacgc
    22681 cctgttcggt gattttacga atatcaccaa ggctgaagac ctggaagccg ccggagtcgg
    22741 taagaatcgg tcctttccac tgcataaaat cgtgcagatc gccgtgcagt ttcatgattt
    22801 cctggcccgg gcgcagccac aggtggaagg tgttgccgag gataatttgc gcgccagtgg
    22861 cttcaacttc ttccggcgtc atccctttta cggtgccgta ggtgccaaca ggcataaaac
    22921 aaggcgtttc cactacgcca cgatcaaaga ccaggcggcc acggcgtgcg cgaccgtcgg
    22981 tggtgtccag ttcaaatttc attttttctc cactacgtca gaaaaacagt ccaacgttta
    23041 aaccagcgcc gcggaattac tccccgacgc gctcattaat tgcctgcgga ttgtacgtga
    23101 taaacatcgc atcaccgtaa ctaaaaaagc gatatttctc ttctaccgcc gctttatagg
    23161 cattcatggt gtgttgataa ccggcaaagg ccgaaaccag cataatcagc gtcgactctg
    23221 gcaagtggaa gttcgtcacc agcgcatcga ccactttgta ctggaagccc ggatagataa
    23281 agatttgggt atcgtcaaag aacggttcga taagatcgtt ttttgccgcc tgtgccgcgc
    23341 tttccagcga acgtactgaa gtggtgccaa ccgcaatcac ccggttaccg cgcgctttcg
    23401 ccgccagtac cgcgtctacc acatcctgcg gtacttcagc gtattccgag tgcatgatgt
    23461 gatcttcaat ggtgtcgacg cgcaccggct ggaaggtgcc cgcaccaacg tgcaacgtca
    23521 caaacgccat ctccacgcct ttggcgcgta atttttccag caaaggctcg tcaaaatgca
    23581 gaccagcggt cggggctgca accgcgcccg gtttttcgct ataaacggtt tgataaagtt
    23641 cgcggtcagc gtcttcgtcc ggacgatcga tatacggcgg cagcggcata tggccgatgc
    23701 tgttgaggat atccagtacc gagcgttcat cattaaattc gacttcaaac agtgcgccgt
    23761 ggcgcgcggt cattgttgcg ttgatacttt cgtcatcgcc cagcaacagt tctgcgccag
    23821 gttttggcgc tttcgaggcg cgaatatgcg caagaatgcg tttgtcgtcg agcatccgct
    23881 caaccagcac ttcaatcttg ccgccgctgg ctttacgccc aaacagacgc gccgggatca
    23941 cgcgggtatt attaaaaacc agaagatcgc cggggttgag cttatcgagt aaatcggtga
    24001 aagtaccgtg cgtcagcgcg cccgtcggcc cgtccagcga cagtaaacga cagctactgc
    24061 gttcaggcat gggatagtgg gcaatcaggg attcgggcaa ttcaaaggag aaatcggtaa
    24121 cgcgcatgac actgactcag actaaaataa gagtcgggta gtctagtgcc ggggcgctct
    24181 ccctgcaacc tttacccccc tttggataga atataatcga tgaatttttt agctcacctg
    24241 catttagccc atctcgcgga aagctcgctt tctggcaatt tactggctga tttcgtacgc
    24301 ggaaatcctg aagaaagttt tccgcccgac gtcgtggctg gcattcatat gcatcgacgt
    24361 attgactgac aatctgccgg aagtccgcga agcacaggag tggtttcgta gtgaaacgcg
    24421 ccgcgttgcg cctattacgc tggatgtcat gtgggatcac tttctttccc gccactggtc
    24481 gcagctgtcg ccggactttc cgctacagga atttgtctgt tatgcccgcg agcaagtgat
    24541 gacgattttg ccggactcac cgccacgttt tatcaatctg aacaattact tgtggtcaga
    24601 gcagtggctg gtgcgctatc gcgatatgga tttcatccag aacgtgttaa acggcatggc
    24661 aagccgccgc ccacgtctgg atgccctgcg tgactcctgg tacgatttag acgctcatta
    24721 tgacgccctc gaaacccgct tctggcagtt ttatccgcgg atgatggcgc aggcgtcaca
    24781 caaggcgtta taaaacgtta gttcatccat accgtagccg aaatggcagg caaagccaga
    24841 atcccgtcag tcagttgccc atgcccttct ttgcattgcc attgcacggc attgagaaac
    24901 ggtgacgcgg gtagcaccac ttcactggcc tcgccacggt tgattgccac cagtacacgt
    24961 tgctgattca gcacgcggac aaataccact acgttatctt ccgcatacag cacctgacag
    25021 ccgccacgac gcagcgcctg acttttctta cgcagcacaa tcattcgccg gtacagcgcg
    25081 aataacgccg tatcctgctt ttccacctgc caggggaacg gtttacggca aaacggatcg
    25141 tttttaccat ccagtcccac ttcatcaccg taataaatgc acggtacacc aggccaggtg
    25201 aacagccaga ccaccgccag cggcaggcgc gcaatatccc gaccgagcag cgttttaaat
    25261 cgcgcagtat cgtggctatc gagctgatta aacatacgta attgttgttg atgagaaagc
    25321 cctgcgcggt aattatccat ccaggccata caggtttggg cgtcaatttg ctgcggatca
    25381 taagagatgt cggtattggc aagaaatccc cacaacggga atgtgaagcc acgatagttc
    25441 atggcagcat cttccacatc ggcctgtaac cattgccgtg catcgccaaa atgttcgccg
    25501 acaatatacg cttccggctg ggtttctttc gccgcttcgg tgatcccggc aacgtgctgc
    25561 atattattgc gcgccccacc cgcctccccc agcatatgca ccacatccag ccgccagccg
    25621 tccatattcc acggcgcttt cagccagtgg cggacaatac tgtcttcccc gcgataaatt
    25681 tcattcacca gactttccga ctgataatcc agcttcgtca agctggcata gccaagccag
    25741 tcgagcgccg tgccatcatc actaaacgag taccagtcgc gccagggcga ttcagagttg
    25801 tgacaagctc cccccgtgcc aagattgtgc ctgtcaaacc aggcatggga atcgccactg
    25861 tggttaaaca cgccgtccag caccagccgc attcccagct gctgcgtatt gtgtcgcaaa
    25921 cgcagcaacg ccccatcacc gccaaactgc ggatcgacat ggcgataatc ctcggtatcg
    25981 tatttatgta cgctgggagc tttaaacacc ggattgagat acagcgctgt cacgccaagc
    26041 tttttcagat acggcagttt ttcgcttatc ccgtccagat cgccgccata gaacgttgat
    26101 ccgcccgcct gcgccgtgac cggttcatcc cagtcacgca agatgatctc ttgtccggct
    26161 gcatgatggt aatagacatg atcctgttca gcttcacgag gaagactacg cgcaaaacga
    26221 tcagggaaga tctgataaaa aatctgatcc gcagcccatt gtgggccgat atcaggtaca
    26281 tcgacggcaa actgctccag tcgtgccggc ggcattcggc tgaagccctg cggtgtaaac
    26341 caacgctggc gatcgtgcca cagcagtttg aaactgtaac gccgccgggg ttgtccgctg
    26401 gagagatcaa tcgccgcacg ccatgcggtg acgcctggct gcggctgact gcgctgctta
    26461 tgcattggta ctgacatttc ttcgttatcg tgttctgtac gcagcataat gcgctgcggt
    26521 gggtcttcgc ccgtcagcca cagtgtaatg agcagttgat ctttgctttg tttaacaaat
    26581 gggggcaccg gcaggtgcca tgcatttaac atcataattc ccctgtgatg aaaatctgat
    26641 caccttgcca tagcctaatg caggatcact catcatctga aggatatttg ggggaggagg
    26701 acgggggcgg ataactttcg gaatagccgg atcaggattt cctcctgaac cggcttacag
    26761 aacgcaagta ttattggttt tcagccagct gacgatcgtg gcggcgttta aacatccagc
    26821 caatcaacag cacaacaatc cacgcgaaac cgacatacag cgagatacgc gtatccgggt
    26881 gataaccaat caacccgata ataaagagca ggaaaatcag cccgccgatg gtcgttgcta
    26941 ccccacccgg cactttaaat ttcagcgcct taacttcttc tggcggcaaa cggcgacgga
    27001 aggcgatttg cgacagcagg atcataatcc acacccatac cgtggcgaag gttgccagcg
    27061 aagcgatcac caggaagacg ttttccggca tgatgtagtt cagatacacc gcaaacagca
    27121 gcgcggtagt catcaccagc accgtaaccc acggaatacc gcgacgcgac gttttgctga
    27181 aaattttcgg tgcgctgccc tgctctgcca taccgtggag catacggcct acgccaaata
    27241 cgtcactgtt aattgccgac agcgaagcgg tcagcacaac aaagttaaga atgctggcgg
    27301 caaaggtaat gcccatatgc tggaacgtca gtacgaacgg gctaccggca gtgccaacct
    27361 gattccacgg gtagatagac ataatgacga acagcgtacc gacgtagaac accagaatac
    27421 gcatcggcac ggagttaatc gcacgcggaa tcgatttctc aggatctttc gcttcaccgg
    27481 cggtaatccc gataatttcg atcccaccgt aagcaaacat caccatttgc aacgacatta
    27541 ccatgccaag ccagccgtta ctgaagaagc cgccgttgct ccacaggtta tgaataccgg
    27601 tcggttgccc gccgttgcca atcccccaga tgatgatgcc gaaaccggcg acaatcatga
    27661 tgatgatggt ggcgacttta aagaacgaga accagaattc cagctcaccg aagaccttca
    27721 cgctcatcag gtttacggcg caaatgatca gcaccacgct cagtacccaa atccagtgcg
    27781 gcaccgtcgg gaacaagaca cccatataga taccaaaagc ggtcacatcg gcgatggcga
    27841 caataaggat ttcaaaacag taggtccatc cggtaatgta acctgccagc gggccaaggt
    27901 tttcctgcgc ataacgcgag aaagagctgg cggccgggtt atgtaccgac atttccccca
    27961 gcgcacgcat aatgatatac gccgcgatac caccgataat ataggccaac aacacgctcg
    28021 gaccggccat tttgatggcg tctgccgaac cgtaaaacag cccggtgcca attgctgaac
    28081 ccagtgccat aaagcgtatg tggcgggtac ttagcccacg ctttagctta ttcttacttt
    28141 ccatcaattc ctaacccatc aacacaataa aaaaccacgg ggcatcgcag ccccgtggtt
    28201 aaaacaaatg ttcagtgatt tagtgagcgc tggaggtcac ctgacgacct gccgcgcgat
    28261 cccagataat ggccagaacc accatcacca ctgttggcat taaccacgcc agaccttgtt
    28321 cggccagcgg taaacgctgc gcccaggacg gtaagatatc gctgaatgca gatgctttga
    28381 tcccgtcgag aataccaaaa agcaggctga taaacatcgg cggagcaatc acgcgggacg
    28441 aattatgcca ccatgagcgt gtaaaactta atacaaccag tgcgatacac ggcggataaa
    28501 tagcggtcag caccggtacg gagatctgga tcagctggct taagccgagg ttagacacca
    28561 ccatcgagaa gccgccgagg ataaacacca gcgtacgata agagagcggt acgtactggg
    28621 caaagaattc tgcacaagca caggtcaggc caaccgccgt gaccagacag gcgatgaaga
    28681 ttaacgccgc cagcaggaag ctaccgccgc cgccaaaggt gtgctgaacg taagcatgca
    28741 gaatagcagc gccgtttgca gactgatcga ccagcgacgc gctgtctgaa cccagacaga
    28801 acagcgccag gtacagcaaa gtcagaccaa cacccgccat caggccagcc cagacggtat
    28861 aacgggtcag cagacgcgct tcggtaacgc cacgagaacg cgccgcgtta acaataacga
    28921 taccaaacac cattgcgccc agcgtatcca tggtcagata gccgttaacg aagccgttag
    28981 aaaatgcagc gttttgataa gcctcagtcg ccgtgctgat agaacccgcc ggccagacaa
    29041 tcgcgacaac agacaggatg accagcgcga taattttcag cggcgcaagg aagttgccca
    29101 cggtatccag cagcttgccc ggatagagcg aaaccagaat aacgatagcg aaatagacca
    29161 ggctgtagat aaacagcggc agcgcggaat cacccgtcag cggcgcaatc cccacttcaa
    29221 aggaaacggt agctgtacgc ggcgtagcga aaagcggccc caccgccagg taacaaaccg
    29281 ttgccagcag tacgccagcg actttaccga ttggcgtgct gaggctgtca acaccgccgc
    29341 caacttttgc cagcgccact accgttaata ccggcaggcc aacggcagta atgaggaagc
    29401 cgaatgccgc agtccagacg tgttcgcctg cctgtaagcc gaccattgga gggaaaataa
    29461 tgttacctgc gccgacgaac aacgcaaatg tcataaagcc cagagcgatg atatcgcgcg
    29521 atcttaattg atgggtcata aatcatactg cctgtggatg tggtgttgta acgttaaatt
    29581 tttgtgccaa tccctgggga tgatacagcg gtatatttaa acaattttag cgggaaatac
    29641 tcccaaccag gcgcggcgaa gaatagtttt tcgatttacc gcgttaatgc agtcagtatg
    29701 acagcacctg aagcgcaatt taaacgctta taacgtttta aagcaaggag ggatctaaaa
    29761 accagaggta tatagcgata agaaatcacc aaccagtata aaacactatt tccgtgaaaa
    29821 agacatggct aatcatgcga acaaaaaatc accaaccgtt gtaaaatcta acgcccgtat
    29881 tttgactgca aagaaaataa gccagcatcg gctggcttat ggcaataaaa gatgacaaag
    29941 gcggattaat cgctgttttt ggcaattaaa cgttccggga taacaaaact gaaacgtgtt
    30001 ccttttccta ctgtactctc aatattcagg cgactttcgt gatgattcac cgcatgtttc
    30061 acgatcgcta accctaatcc gctaccgccg gtttgccggg aacgcgcttt atcaacgcga
    30121 taaaaacgct cggtcaggcg cggaatatgc tccggtgcaa tgcccggtcc gttatcttca
    30181 acgctaaatt cggcaccgtg cggcactcgc tgccagcgta cggtgatatg cgtgccttcc
    30241 ggcgtatgat tcacggcgtt atagaccagg ttcgaaatcg cactgcgtag ctgatcttcg
    30301 ttgccagaca ccttgaggcc gttatctatc tcaaaggtaa atgtctgttt tttctgactc
    30361 agagtctgag cctcgcgctc aacaacgcgc agcatcatcg gcacatcaac cttttcattg
    30421 agcaattgcg tcggcgcggc ttcaattttc gacagcgtta gcaactgctt caccagccct
    30481 tccatccgct gggtctgctc gcgcatggtg tgcaacgctt tttcgcgtac cgcgccttcc
    30541 agcggctgct catccatcat ctccaggtaa ccctgtaaca cggtcaatgg cgtacgtaac
    30601 tcatggctca cgttggcaaa aaagttacgc cgcgcccctt ccagttgatg catttgcgtg
    30661 acatcacgcg ccaccatcag caactgtttg tgggtataag gcatgacgcg aatttccaga
    30721 tgccgcccgg tgttgagcac cagattgagc gggcgagaaa aatcacgcgt tttcagatat
    30781 tgcgtaaact ccgggtaacg cagtaggtta aggatgttct gcccgttatc ttccggccag
    30841 cgcaaaccaa gaatttgttg cgccagaccg ttacaccaga aaataccgcc ctcttccgtg
    30901 gtcagcacca ccgcgtcggg cagcgactcc gcgccgctac gaaagcgttt aatcagattg
    30961 cccagttcac ggcggcgttt tttatttcgc agctgcatct ggtgtaagcc gtatagtagc
    31021 ggttcccagc taccacgccc cggtggcggg gtcatactgc gatccaccca cagccaccat
    31081 gaaaggcgca ataaattcca gaaatgccag ataagcagtc ctgttaccga tgccagcaaa
    31141 aaccagggca ggtaaccaaa aaatgcaccc aggatgaaag ccgggaggca gcaaagtagc
    31201 agctccagca ccagcctttt ccacgacagc cgttccagca cgtaagatac tccagttaag
    31261 aaatcataag ccctgctctg cgtccgatga gcaaggcgtt aaaagcgggt tgaaaaacga
    31321 tatcctgtac cgcgcacggt ctgcaccatg cggtcatgcc cgccgggctc cagtgcttta
    31381 cgcaggcgac gaatgtggac atcgaccgtg cggtcttcca catacacgtt agttccccag
    31441 acgtggttta acagctgctc gcggctgtac acgcgctcag gatgcgtcat aaaaaagtgc
    31501 agcagtttaa attctgtcgg ccccatctcc agcggctctt cgcccgccat cactcggtga
    31561 gatgtcgggt tgagacttaa tccctgcatc ttaatcacct cttccaccgc cattggcgaa
    31621 atacggcgca ttaccgcttt gattcgcgcc accagctcct tcggcgaaaa tggcttggtg
    31681 atatagtcat ccgcgccggt ttcaaggccg cgcacgcgat cttcttcttc ccctctggcg
    31741 gtcaacatca ccactggaat atcccgggtc atcgactcgc gcttgaggtg tttgatgaac
    31801 tggataccgg agccgccagg taacatccag tcaaggagaa ttaaatccgg ccagggttca
    31861 ttcagttgat tcacagcact gtcataatct tccgcttcga ccggctgaaa gccattttgt
    31921 tcgagcacga agcagaccat ttcgcgaatt ggagcttcat cttctacgac cagaatacgt
    31981 ctcgccatga tttgccctgt tgtaataaat aggttgcgat cattaatgcg acgtcattat
    32041 gcgtcagatt tatgacagat ttatgaaaag ctcgtcgcac atatcttcag gttattgatt
    32101 tccgtggcgc agaaaaaagc aaatggcaca tctgtttggg tataatcgcg accatgcttt
    32161 ttcgccaggg aaccgttatg cgcatccttc acacctcaga ctggcatctc ggccagaact
    32221 tctacagtaa aagccgcgaa gctgaacatc aggcttttct tgactggctg ctggagacag
    32281 cacaaaccca tcaggtggat gcgattattg ttgctggtga tgttttcgat accggctcgc
    32341 cgcccagtta cgcccgcacg ttatacaacc gttttgttgt caatttacag caaactggct
    32401 gtcatctggt ggtactggca ggaaaccatg actcggtcgc cacgctgaat gaatcgcgcg
    32461 atatcatggc gttcctcaat acaaccgtgg tcgccagcgc cggacatgcg ccgcaaatct
    32521 tgcctcgtcg cgacgggacg ccaggcgcag tgctgtgccc cattccgttt ttacgcccgc
    32581 gtgacattat ttccagccag gcggggctta acggtattga aaaacagcag catttactgg
    32641 cagcgattac cgattattac caacaacact atgccgatgc ctgcaaactg cgcggcgatc
    32701 agcctctgcc catcatcgcc acgggacatt taacgaccgt gggttccagt aaaagtgacg
    32761 ccgtgcgtga catttatatt ggcacgctgg acgcgtttcc ggcacaaaac tttccaccag
    32821 ccgactacat cgcgctcggg catattcacc gcgcacagat tattggcggc atggaacatg
    32881 ttcgctattg cggttccccc attccactga gttttgatga atgcggtaag agtaaatatg
    32941 tccatctggt gacattttca aacggcaaat tagagagcgt ggaaaacctg aacgtaccgg
    33001 taactcaacc catggcggtg ctgaaaggcg atctggcgtc gattaccgca cagctggaac
    33061 agtggcgcga tgtatcgcag gagccacctg tctggctgga tatcgaaatc actactgatg
    33121 agtatctgca tgatattcag cgcaaaatcc aggcattaac cgaatcattg cctgtcgaag
    33181 tattgctggt acgtcggagt cgtgaacagc gcgagcgtgt gttagccagc caacagcgtg
    33241 aaaccctcag cgaactcagc gtcgaagagg tgttcaatcg ccgtctggca ctggaagacc
    33301 tggatgaatc gcagcagcaa cgtctgcagc atcttttcac cacgacgttg cgtaccctcg
    33361 ccggagaaca cgaagcatga aaattctcag cctgcgcctg aaaaacctga actcattaaa
    33421 aggcgaatgg aagattgatt tcacccgcga gccgttcgcc agcaacgggc tgtttgccat
    33481 taccggccca accggtgcgg ggaaaaccac cctgctggac gccatttgtc tggcgctgta
    33541 tcacgaaact ccgcgtctct ctaacgtttc acaatcgcaa aatgatctca tgacccgcga
    33601 taccgctgaa tgtctggcgg aggtggagtt tgaagtgaaa ggtgaagcgt atcgcgcgtt
    33661 ctggagccag aatcgggcgc gtaaccagcc ggacggtaat ttgcaggtgc ctcgcgtaga
    33721 gctggcgcgc tgcgccgacg gcaaaattct cgccgacaaa gtgaaagata agctggaact
    33781 gacagcgacg ttaaccgggc tggattacgg gcgcttcacc cgttcgatgc tgctttcgca
    33841 ggggcaattt gctgccttcc tgaatgccaa acccaaagaa cgcgcggaat tgctcgagga
    33901 gttaaccggc actgaaatct acgggcaaat ctcggcgatg gtttttgagc agcacaaatc
    33961 ggcccgcact gagctggaga agctgcaagc gcaggccagc ggcgtcacgt tgctcacgcc
    34021 ggaacaagtg caatcgctga cagcgagttt gcaggtactt actgacgaag aaaaacagtt
    34081 aattaccgcg cagcagcaag aacaacaatc gctaaactgg ttaacgcgtc aggacgaatt
    34141 gcagcaagaa gccagccgcc gtcaacaggc cttgcaacag gcgttagccg aagaagaaaa
    34201 agcgcaacct caactggcgg cgcttagtct ggcacaaccg gcacgaaatc ttcgtccaca
    34261 ctgggaacgc atcgcagaac acagcgcggc gctggcgcat attcgccagc agattgaaga
    34321 agtaaatact cgcttacaga gcacaatggc gcttcgcgcg agcattcgcc accacgcggc
    34381 gaagcagtca gcagaattac agcagcagca acaaagcctg aatacctggt tacaggaaca
    34441 cgaccgcttc cgtcagtgga acaacgaact ggcgggttgg cgtgcgcagt tctcccaaca
    34501 aaccagcgat cgcgagcatc tgcggcaatg gcagcaacag ttaacccatg ctgagcaaaa
    34561 acttaatgcg cttgcggcga tcacgttgac gttaaccgcc gatgaagttg ctaccgccct
    34621 ggcgcaacat gccgagcaac gcccactgcg tcagcgcctg gtcgcgctgc atgggcagat
    34681 tgttccccaa caaaaacgtc tggcgcagtt aatggtcact atccagaatg tcactctgga
    34741 acagacgcaa cgtaatgtcg cacttaacga aatgcgccag cgttataaag aaaagacgca
    34801 gcaacttgcc gatgtgaaaa ccatttgcga gcaggaagcg cgcatcaaaa cgctggaagc
    34861 ccagcgtgca cagttacagg cgggtcagcc ttgtccactt tgtggttcca ccagccaccc
    34921 ggcggtcgag gcgtatcagg cgctggagcc tggtgttaat cagtctcgat tactggcgct
    34981 ggaaaacgaa gttaaaaagc tcggcgaaga aggtgcggcg ctgcgcgggc aactggatgc
    35041 attaacgaag cagcttcagc gcgatgaaaa cgaagcgcaa agcctccgac aagatgagca
    35101 agcacttact caacaatggc aagccgtcac ggccagcctc aatatcacct tgcagccaca
    35161 ggacgatatt caaccgtggc tggatgcaca agatgagcac gaacgccagc tgcggttact
    35221 cagccaacga catgaattac aagggcagat tgccgcgcat aatcagcaaa ttatccagta
    35281 tcaacagcaa attgaacaac gtcagcagca gctcttaacg gcattggcgg gttatgcact
    35341 gacattgcca caggaagatg aagaagagag ctggttggcg acacgtcagc aagaagcgca
    35401 gagctggcag caacgccaga acgaattaac cgcgctgcaa aaccgtattc agcagctgac
    35461 accgattctg gaaacgttgc cgcaaagtga tgatctcccg cacagcgaag aaactgtggc
    35521 gctggataac tggcggcagg ttcatgaaca atgtctcgca ttacacagcc agcagcagac
    35581 gttacagcaa caggatgttc tggcggcgca aagtctgcaa aaagcccaag cgcagtttga
    35641 caccgcgcta caggccagcg tctttgacga tcagcaggcg ttccttgcgg cgctaatgga
    35701 tgaacaaaca ctaacgcagc tggaacagct caagcagaat ctggaaaacc agcgccgtca
    35761 ggcgcaaacg ctggtcactc agacagcaga aacgctggca cagcatcaac aacaccgacc
    35821 tgacgggttg gctctcactg tgacggtgga gcagattcag caagagttag cgcaaactca
    35881 ccaaaagttg cgtgaaaaca ccacgagtca aggcgagatt cgccagcagc tgaagcagga
    35941 tgcagataac cgtcagcaac aacaaacctt aatgcagcaa attgctcaaa tgacgcagca
    36001 ggttgaggac tggggatatc tgaattcgct aataggttcc aaagagggcg ataaattccg
    36061 caagtttgcc caggggctga cgctggataa tttagtccat ctcgctaatc agcaacttac
    36121 ccggctgcac gtgcgctatc tgttacagcg caaagccagc gaggcgctgg aagtcgaggt
    36181 tgttgatacc tggcaggcag atgcggtacg cgatacccgt accctttccg gcggcgaaag
    36241 tttcctcgtt agtctggcgc tggcgctggc gctttcggat ctggtcagcc ataaaacacg
    36301 tattgactcg ctgttccttg atgaaggttt tggcacgctg gatagcgaaa cgctggatac
    36361 cgcccttgat gcgctggatg ccctgaacgc cagtggcaaa accatcggtg tgattagcca
    36421 cgtagaagcg atgaaagagc gtattccggt gcagatcaaa gtgaaaaaga tcaacggcct
    36481 gggctacagc aaactggaaa gtacgtttgc agtgaaataa ctattcagca ggataatgaa
    36541 tacagagggg tgaattatct cttggccttg ctggtcgtta tcctgcaagc tatcacttta
    36601 ttggctacgg tgattggtag ccgttctggt ggttgtgatg gtggtatgaa aaaagtcatt
    36661 ttatctttgg ctctgggcac gtttggtttg gggatggccg aatttggcat tatgagcgtg
    36721 ctcacggagc tggcgcataa cgtaggaatt tcgattcctg ctgccgggca tatgatctcg
    36781 tattatgcac tggtggtggt ggtcggtgcg ccaatcatcg cactcttttc cagccgctac
    36841 tcactcaaac atatcttgtt gtttctggtg gcgttgtgcg tcattggcaa cgccatgttc
    36901 acgctctctt cgtcttacct gatgctcgcc attggtcggc tggtatccgg ctttccgcat
    36961 ggcgcatttt ttggcgtcgg agcgatcgtg ttatcaaaaa ttatcaaacc cggaaaagtc
    37021 accgccgccg tggcggggat ggtttccggg atgacagtcg ccaatttgct gggcattccg
    37081 ctgggaacgt atttaagtca ggaatgctgg cgttacacct ttttattgat cgctgttttt
    37141 aatattgcgg tgatggcatc ggtctatttt tgggtgccag atattcgcga cgaggcgaaa
    37201 ggaaatctgc gcgaacaatt tcactttttg cgcagcccgg ccccgtggtt aattttcgcc
    37261 gccacgatgt ttggcaacgc aggtgtgttt gcctggttca gctacgtaaa gccatacatg
    37321 atgtttattt ccggtttttc ggaaacggcg atgaccttta ttatgatgtt agttgggcta
    37381 gggatggtgc tgggaaatat gctaagtggc aggatttcag gacgttattc accactgcgc
    37441 attgcagcag tgactgactt tatcattgta ctggcactgc tgatgctctt tttctgcggc
    37501 ggcatgaaaa caacgtcgct tatttttgct tttatttgtt gcgcgggatt atttgccctt
    37561 tcagcacctc tgcaaatatt gttactgcaa aatgccaaag gaggagagtt attaggtgcc
    37621 gcaggtgggc aaatagcgtt taacctcggt agcgccgtcg gcgcatattg cggaggtatg
    37681 atgctgacgc tggggctggc atataattac gtggcgctgc ctgccgccct gctttcgttt
    37741 gctgcgatgt catcgttgct gctgtatggt cgctataagc gccagcaagc ggcggatagt
    37801 ccggtgctgg cgaaaccact ggggtagatt actgtcacgg tgcgcgttgc ttattgccgg
    37861 atgcggcgtg aacgccttat ccgccctacg cggttctggc acattttgta ggcctgataa
    37921 gacgcggcaa gcgtcgcatc aggcatgatg cgccaattgc ctacgttttt tactcttgtg
    37981 gccataacca cgcagcgccg cgtacgccgc tggaatcacc gtgcttcgcc ttactcaccg
    38041 gcgtttcaca ttcgccgccg aagacaaatt gtttaatcaa ctgcccaacc gtttgatata
    38101 aacggtctac attgctcatc ccgcccccca ggacaatcac atccggatcg agaatattca
    38161 cgacatgtgc cagcgatttt gccagccgca gctcgtagcg acgcaatgcc agttccgcta
    38221 ccggatcgct ttcttcaacc aggcggataa tttcactgcc tttcagcgca tgtccgctca
    38281 aacgacgata atccgtcgcg aatcccgtgc ccgaaataaa ggtttcaata caaccttgtt
    38341 taccgcaata acaagggact tcctcgcgat aacgcagttc gtcttcgtcc atccacggta
    38401 gcggattgtg tccccactca cctgccgtgc cattgccgcc gatatgcgtc cgcccattga
    38461 atgccacgcc cgcgccgcat cccgtgccga taatcacggc aaataccgtc tgcgctcccg
    38521 ctgccgcgcc atctactgct tctgaaaccg ccagacagtt ggcatcattt gccagccgca
    38581 cttcccgctg caacctcgcg cttaagtctt tatcgaatgg ctgaccgttg agccaggttg
    38641 aattgacatt cttcaccaca ccggtgtagg gcgaaatcga acctggaatg cccataccta
    38701 ccgttccgcg ctgccctgtc gcctgctccg ccatatcaac caacgtggcg atcgtttcaa
    38761 tagtctgccg gtaatcatca cgcggcgtgg gcagacgatg gcggtacaac tgctcccctg
    38821 catcgcccag tgcaatcact tcagttttgg tgccgcctaa atcgatacct atacgcactg
    38881 tactctcctt atttttttca atatcaatag cgtagagacg gacaaccgga ttggcaatgc
    38941 aaggccgccg acaattcgtt atcatgcccg ctaaatttaa cgacaaggcc gtggaaatta
    39001 tcatgctgtg gttcaaaaat ttaatggttt accgtcttag ccgcgagatt tcgctgcgtg
    39061 cagaagagat ggaaaaacag ctagcctcga tggcatttac cccatgcggc agccaggaca
    39121 tggcgaagat gggctgggtt cctccgatgg gatcgcacag cgatgcgtta acgcacgttg
    39181 ccaatggtca aattgttatc tgcgcgcgca aagaagaaaa aatcctcccg tctccggtga
    39241 ttaaacaggc gctggaagcg aaaatcgcca aactggaagc ggaacaggcg cgtaagctga
    39301 agaaaaccga aaaagattcg ctgaaagacg aagtgctgca ctctctgctg ccgcgtgctt
    39361 tcagccgttt tagccagaca atgatgtggc tcgacacggt taacggtttg attatggtgg
    39421 actgcgccag tgccaaaaaa gcggaagata cgctggcatt actgcgtaaa agcctggggt
    39481 cgttaccggt tgtaccattg agcatggaaa acccgattga actgacgctg accgaatggg
    39541 ttcgctccgg tagtgcggca cagggcttcc agctgcttga tgaagccgag ctgaaatcgt
    39601 tgctggaaga tggcggcgtg atccgcgcga agaaacaaga tctgaccagc gaagagatca
    39661 ccaatcacat tgaagccgga aaagtggtga ctaaactggc gctcgactgg cagcagcgca
    39721 ttcagtttgt gatgtgcgac gatggttcgc tcaagcgtct taagttctgc gacgagctgc
    39781 gcgatcaaaa cgaagatatc gaccgtgaag atttcgccca gcgttttgat gccgatttca
    39841 tcctgatgac tggtgaactg gcagcgttaa ttcaaaacct gattgaagga ttaggtggcg
    39901 aagcacaacg ttaattgctg attttccttt aatgccggat gcgacgcctg ccgcgtctta
    39961 tccggcgtac gaagccacac caggcatata attattcgct acggcgagca ataattttta
    40021 gcgcagcaat attatgcgtt ttacgccgta actgatctaa tcgcctcatc cctattcgcc
    40081 tgtcagcagc acgcagaata ttcactaacc cccatttatc gctggtaaat tgttttgtta
    40141 acagctccgc ttttggggta tcaatatatt cacgcaataa atcagaaatt gaacaaatat
    40201 ccgtcaggta aggataccag caagtttcac cgtgaaaatt acgcactcca gcgttacctg
    40261 caataaagtc tttcgggtaa tcccagatca cgtttttatc caacgtgatc caataacgcg
    40321 gtaaatcggt acttccacca ttttggctgc gcatgggata acgggtacag tgaatttgca
    40381 ggttgatcgt tggcgtcagt aagtcataga tctcccgctg taacttgctc catggacgtt
    40441 gtgtcattgt ttttcctcaa gccggtgacg cgcttcactg cgaattttcc gcgttgcccc
    40501 ggttttcact aaatcacgta actgcgaaag atcgggttgt tcgacaaata agagtaatga
    40561 tggatcctct tttaaccacg ccgttttcac atcggcagcc agctgtggat tattaatagc
    40621 cagcgaccga tcctgaggct cagtcaatgt cgttaacaac gacgccgggg tatgtgcgtt
    40681 gcgtaatgcc gtttggcgca aatgatcatt gcgactttgc agcaatgcac gccaccattc
    40741 atcatctgat ttatctttta tcgtagccag ttccgcaaaa aatacctgga gatcttcact
    40801 gatatttata tcaaccttga tgaattatga atatatcgga aattgaaaaa atcgcagcaa
    40861 aggaaatatt attatgggtt aaagagagga tatttcagaa aaatgccctc ccgttcaagg
    40921 ggaaggcgag gaattacaga tagcggcaca gataagaggt aggttcggca acttgaagat
    40981 gaaactcact gtgaccggga acattaaaca ccgaaccggc ttcatacact tgccagtcgg
    41041 tcgcgtcagg cagtaacaca ttcagcgcgc cactgattac cgtcatctct tccggctcag
    41101 cggtgctgaa ggtgtattcg ccttcaacca taacacccac gctggcgcga ccagtgctgc
    41161 tgctggaaaa gccgatcgat ttcactttgc cggaaaagta ctcattactt tgaagcataa
    41221 actggccctg tctggtagtt gaacatctac ttcactatag gggccagagg cgctggctgt
    41281 cacgcaaaat tacaccagta attccgcagc cagccgtgca atcaatacgt tagagagcaa
    41341 gacaggaaca tcgagctgtt tttgcagtaa atcgcgatga cgttggtgaa atcctaaaca
    41401 atccagcatg atgacatcag ccccttttgc cagtaattct ttcccagcat caatgatttt
    41461 ttgttctgaa tcatgaatgg ggttacccaa tgaaaatacc ggcgatttct gcaaaatttg
    41521 ccatttttgc gcctgaacgg gcagcatctc ctcaaccgga acgataaccc ccacctgatg
    41581 atcttcaaca atagaggaaa ccagtggagg caatattcgc gacggctcaa gaaagatcgt
    41641 attacgcgca gtcatactac taatgtttgc tgtactcatt aataaaatga cgtcataacc
    41701 ctgattatcg agcacttcaa ccacaccttg cagatctcgc tccactttac gacgcgaaac
    41761 atgggccagc tggttgtcat ttaataatgt gagaatggtg tcttcgcctg cttctggcgc
    41821 gtactccgcc atcacttctt cacgacttaa cttccccagc aggctatgat gggaaatatt
    41881 atcttcgtca atgtattccg tcaggagcgg caaaacctcc tgcataggta caatgccgat
    41941 ggtcaggatc gccaacgacg cactcattgt tcatccacct tacttttctt tcgtcgttaa
    42001 ttaccgggca agtgtgaagc accactgctg acacttactt gtgctacaaa tgacaaaaag
    42061 cgtagcagca gacgtgcacg gcataatgta aagattccaa atgattccag taatggaatg
    42121 ttattgttta atattctaat tattagaaaa acataaatta tgaaaaatgt gacgcagatc
    42181 ccttgtagtg gcactcagaa tcccttccga gcagtctgat gcggtcactc tttgtcaggg
    42241 tcctcatagc gttttttcgc atccatcgct tcctgagccg tcggatgttc actgatcaac
    42301 gagtctggtt taggatgatc ggctctgagt tggtaccagg ttaccgtctg tcctggcttt
    42361 cctttttcaa tcgtcactat atatgcttca cgaggataag gcggtttcgt tggcatcgtt
    42421 cttccttatt tcacgggatg aacgttaagt ataggcgctc taaaatcaac aattgatcgt
    42481 ctgtgccagg gcgctacgaa tttcagaaat cacctggctg ggttcgtttg ttgcgtcgat
    42541 gataatatgc gcaacttcgc gatatagcgc atcgcgttct tccagcactt cctgaacttc
    42601 ttcgctcagc ggttttcccg ttaaggttgg ccgtaaatct tcttccggtg cagcttgcag
    42661 tcggttaacc aggactgata ctggcgcaca caaataaacc acgatcccgt tattttgcat
    42721 gaagtgacga ttaaattccg tcagaataat gccgccgcct gtagcgataa cggtggatgc
    42781 cgcagttacc gcttccagcg ccgccgtttc tctggcgcga aatcccgccc actcttccct
    42841 ttcgacgatc tccgcgaccg tcatattgag ctgtgattgc aaccactgat cggtatcgac
    42901 aaaccgacgg ttaagcgaat cggcaagggc cattccgacc gttgttttac cacagccccg
    42961 aggcccgatc agaaaaagag gttgtgtcat cgtgggtttt ccccaatagg tcgcaatgcg
    43021 gcgaaagccg gtgtcatgag aatagcgatc ataccatcaa actagtacaa tttcgattgt
    43081 aaagaaaaaa ttccacttaa agtaaaaatc tcaatacatc ccttactata ccaataaata
    43141 ttcaagaatg aagtgtaaat aataaattac atttagccac gactacgttg cacttccagc
    43201 caccacttct ccagctccgc ggcaaaggcc tggcggtcac gttgtgaaag gctatctggt
    43261 ccgccggtct ggatcccact ggcacgtaag gtatccataa aatcgcgcat cgtcaggcgc
    43321 tcacgaatgg tcgctggcgt gtaacgttcg ccgcgcgaat taagcgccgc tgcgcctttc
    43381 tcgatggctt cagcagccaa aggtatatct gcggtgatca ccaaatcgcc cgcttcacac
    43441 tgccggacaa tttcgttatc ggcaacgtcg aaacctgccg cgacgcgcag cgtacgaata
    43501 aatcgcgatg gcggcacgcg taaactctgg tttgctacca gtaccagcgg catctgcata
    43561 cgttccgccg cgcgatacaa aatctcttta attacattgg gacacgcgtc ggcatccacc
    43621 caaattgtca taaagtcatc ctttgttagg taatcctcta ttgtgtcgcg cttttgcctt
    43681 ccggcatagt tctgtttatg cttctgccag cgattatcaa aacaatgaat ttcacggcag
    43741 gagtgaggca atggaaaaga aaatcggttt tattggctgc ggcaatatgg gaaaagccat
    43801 tctcggcggt ctgattgcca gcggtcaggt gcttccaggg caaatctggg tatacacccc
    43861 ctccccggat aaagtcgccg ccctgcatga ccagttcggc atcaacgccg cagaatcggc
    43921 gcaagaagtg gcgcaaatcg ccgacatcat ttttgctgcc gttaaacctg gcatcatgat
    43981 taaagtgctt agcgaaatca cctccagcct gaataaagac tctctggtcg tttctattgc
    44041 tgcaggtgtc acgctcgacc agcttgcccg cgcgctgggc catgaccgga aaattatccg
    44101 cgccatgccg aacactcccg cgctggttaa tgccgggatg acctccgtaa cgccaaacgc
    44161 gctggtaacc ccagaagata ccgctgatgt gctgaatatt ttccgctgct ttggcgaagc
    44221 ggaagtaatt gctgagccga tgatccaccc ggtggtcggt gtgagcggtt cttcgccagc
    44281 ctacgtattt atgtttatcg aagcgatggc cgacgccgcc gtgctgggcg ggatgccacg
    44341 cgcccaggcg tataaatttg ccgctcaggc ggtaatgggt tccgcaaaaa tggtgctgga
    44401 aacgggagaa catccggggg cactgaaaga tatggtctgc tcaccgggag gcaccaccat
    44461 tgaagcggta cgcgtactgg aagagaaagg cttccgtgcc gcagtgatcg aagcgatgac
    44521 gaagtgtatg gaaaaatcag aaaaactcag caaatcctga tgactttcgc cggacgtcag
    44581 gccgccactt cggtgcggtt acgtccggct ttctttgctt tgtaaagcgc caaatctgcc
    44641 gatttcaacc actcacgata gtgactcatt tgtgggttca gcggcgcaac ccccacacta
    44701 atccgtaaag ttacctgtgg cgtattcggc aagcgtaacg tgtttagccc ttcacgcacc
    44761 cgtaacatgg cggtaatggc gctctcagct ggcgtaccgg acatgattac tgcaaactca
    44821 tcgccgccaa accgaccaat cacatcgcta ccgcgcaggg taatttgtaa ctgtcgggta
    44881 agcgccacaa tcgcttcatc gcccacatca tgaccccagg tatcgttgat gctcttgaaa
    44941 tggtcgatat cgataatcag taacgttgca tcgcgattat gccgccgaca gttatcaaat
    45001 tcattgcgta acatagtttc ccaatgacgt cggttataca cgcccgtcat gccgtcgcga
    45061 gtactcatga cctgcaacct gcgtttatgt tccgccagtt tggttgccgt ctggtagctg
    45121 acccagccaa acatcagagg ataaacgaca ataatgggaa gggagagcca ccattccagc
    45181 ggagcactat tgaacgacac ggtaatgccc gtaagctcga gggtgacaag gcaggaaacc
    45241 accatcaaca ccagacccgc gacaaacaga cgggggccgc ctgcccccat caaattcaga
    45301 cacagaatca tcaacatagc ggtggaaggc agcacgttta cgcccattac gcctacccac
    45361 attcccgcta atactgcatc ggtttttaag ttgtaaattt cccggctaag cggatcgacg
    45421 gccctgctcg ctatctgcca ggctaaatgc ggcc
//
LOCUS       DAJTRC010000024        42657 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.024, whole genome shotgun sequence.
ACCESSION   DAJTRC010000024 DAJTRC010000000
VERSION     DAJTRC010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 42657)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42657)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42657
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.024"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO357_001422"
     CDS             complement(89..625)
                     /gene="ssb1"
                     /locus_tag="OO357_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein SSB1"
                     /protein_id="HCR5886421.1"
                     /translation="MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDK
                     ATGEMKEQTEWHRVVLFGKLAEVASEYLRKGSQVYIEGQLRTRKWTDQSGQDRYTTEV
                     VVNVGGTMQMLGGRQGGGAPAGGNIGGGQPQGGWGQPQQPQGGNQFSGGAQSRPQQSA
                     PAAPSNEPPMDFDDDIPF"
     gene            879..3701
                     /gene="uvrA"
                     /locus_tag="OO357_001423"
     CDS             879..3701
                     /gene="uvrA"
                     /locus_tag="OO357_001423"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HCR5886422.1"
                     /translation="MDKIEVRGARTHNLKNINLVIPRDKLIVVTGLSGSGKSSLAFDT
                     LYAEGQRRYVESLSAYARQFLSLMEKPDVDHIEGLSPAISIEQKSTSHNPRSTVGTIT
                     EIHDYLRLLFARVGEPRCPDHDVPLAAQTVSQMVDNVLSQPEGKRLMLLAPIIKERKG
                     EHTKTLENLASQGYIRARIDGEVCDLSDPPKLELQKKHTIEVVVDRFKVRDDLTQRLA
                     ESFETALELSGGTAVVADMDDPKAEELLFSANFACPICGYSMRELEPRLFSFNNPAGA
                     CPTCDGLGVQQYFDPDRVIQNPELSLAGGAIRGWDRRNFYYFQMLKSLADHYKFDVEA
                     PWGSLSANVHKVVLYGSGKENIEFKYMNDRGDTSIRRHPFEGVLHNMERRYKETESSA
                     VREELAKFISNRPCASCEGTRLRREARHVYVENTPLPAISDMSIGHAMEFFNNLKLAG
                     QRAKIAEKILKEIGDRLKFLVNVGLNYLTLSRSAETLSGGEAQRIRLASQIGAGLVGV
                     MYVLDEPSIGLHQRDNERLLGTLIHLRDLGNTVIVVEHDEDAIRAADHVIDIGPGAGV
                     HGGEVVAEGPLEAIMAVPESLTGQYMSGKRKIEVPKKRVPANPEKVLKLTGARGNNLK
                     DVTLTLPVGLFTCITGVSGSGKSTLINDTLFPIAQRQLNGATIAEPAPYRDIQGLEHF
                     DKVIDIDQSPIGRTPRSNPATYTGVFTPVRELFAGVPESRARGYTPGRFSFNVRGGRC
                     EACQGDGVIKVEMHFLPDIYVPCDQCKGKRYNRETLEIKYKGKTIHEVLDMTIEEARE
                     FFDAVPALARKLQTLMDVGLTYIRLGQSATTLSGGEAQRVKLARELSKRGTGQTLYIL
                     DEPTTGLHFADIQQLLDVLHKLRDQGNTIVVIEHNLDVIKTADWIVDLGPEGGSGGGE
                     ILVSGTPETVAECEASHTARFLKPML"
     gene            complement(3736..4092)
                     /locus_tag="OO357_001424"
     CDS             complement(3736..4092)
                     /locus_tag="OO357_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5886423.1"
                     /translation="MTISELLQYCMAKPSAEQSVHNDWKATQIKVEDVLFAMVKEVEN
                     RPAVSLKTSPELAELLRQQHSDVRPSRHLNKAHWSTVYLDGSLPDSQIYYLVDASYQQ
                     AVNLLPEEKRKLLVQL"
     gene            complement(4096..4512)
                     /locus_tag="OO357_001425"
     CDS             complement(4096..4512)
                     /locus_tag="OO357_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbQ family protein"
                     /protein_id="HCR5886424.1"
                     /translation="MWYQKTLTLSAKSRGFHLVTDEILNQLADMPRVNIGLLHLLLQH
                     TSASLTLNENCDPTVRHDMECFFLRTVPDNGNYEHDYEGADDMPSHIKSSMLGTSLVL
                     PVHKGRIQTGTWQGIWLGEHRIHGGSRRIIATLQGE"
     gene            complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO357_001426"
     CDS             complement(4623..5336)
                     /gene="aphA"
                     /locus_tag="OO357_001426"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid phosphatase AphA"
                     /protein_id="HCR5886425.1"
                     /translation="MRKITQAISAVCLLFALNSSAVALASSPSPLNPGTNVARLAEQA
                     PIHWVSVAQIENSLAGRPPMAVGFDIDDTVLFSSPGFWRGKKTFSPESEDYLKNPVFW
                     EKMNNGWDEFSIPKEVARQLIDMHVRRGDAIFFVTGRSPTKTETVSKTLADNFHIPAT
                     NMNPVIFAGDKPGQNTKSQWLQDKNIRIFYGDSDNDITAARDVGARGIRILRASNSTY
                     KPLPQAGAFGEEVIVNSEY"
     gene            5738..5942
                     /locus_tag="OO357_001427"
                     /pseudo
     CDS             5738..5942
                     /locus_tag="OO357_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165333.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OO357_001428"
     CDS             complement(6445..7638)
                     /gene="tyrB"
                     /locus_tag="OO357_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid transaminase"
                     /protein_id="HCR5886426.1"
                     /translation="MFQKVDAYAGDPILTLMERFKEDPRSDKVNLSIGLYYNEDGIIP
                     QLKAVAEAEARLNAQPHGASLYLPMEGLNSYRHAIAPLLFGADHPVLQQQRVATIQTL
                     GGSGALKVGADFLKRYFPESGVWVSDPTWENHVAIFAGAGFEVSTYPWYDEVTNSVRF
                     NDLLATLKTLPARSIVLLHPCCHNPTGADLTNDQWDAVIEILKARELIPFLDIAYQGF
                     SAGMEEDAYAIRAIASAGLPALVSNSFSKIFSLYGERVGGLSVLCEDAEAAGRVLGQL
                     KATVRRNYSSPPNFGAQVVAAVLNDEALKASWLAEVEEMRTRILAMRQELVKVLSTEM
                     PERNFDYLLNQRGMFSYTGLSAAQVDRLREEFGVYLIASGRMCVAGLNTANVQRVAKA
                     FAAVM"
     gene            complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OO357_001429"
     CDS             complement(7891..8970)
                     /gene="alr"
                     /locus_tag="OO357_001429"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HCR5886427.1"
                     /translation="MQAATVVINRRALRHNLQRLRELAPASKMVAVVKANAYGHGLLE
                     TARTLPDADAFGVARLEEALRLRAGGITKPVLLLEGFFDARDLPTISAQHFHTAVHNE
                     EQLAALEEASLDEPVTVWMKLDTGMHRLGVRPEQAEAFYHRLTQCKNVRQPVNIVSHF
                     ARADEPKCGATEKQLAIFNTFCEGKPGQRSIAASGGILLWPQSHFDWVRPGIILYGVS
                     PLEDRSTGADFGCQPVMSLTSSLIAVREHKAGEPVGYGGTWVSERDTRLGVVAMGYGD
                     GYPRAAPSGTPVLVNGREVPIVGRVAMDMICVDLGPQAQDKAGDPVILWGEGLPVERI
                     AEMTKVSAYELITRLTSRVAMKYVD"
     gene            complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OO357_001430"
     CDS             complement(9023..10438)
                     /gene="dnaB"
                     /locus_tag="OO357_001430"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5886428.1"
                     /translation="MAGNKPFNKQQAEPRERDPQVAGLKVPPHSIEAEQSVLGGLMLD
                     NERWDDVAERVVADDFYTRPHRHIFTEMARLQESGSPIDLITLAESLERQGQLDSVGG
                     FAYLAELSKNTPSAANISAYADIVRERAVVREMISVANEIAEAGFDPQGRTSEDLLDL
                     AESRVFKIAESRANKDEGPKNIADVLDATVARIEQLFQQPHDGVTGVNTGYDDLNKKT
                     AGLQPSDLIIVAARPSMGKTTFAMNLVENAAMLQDKPVLIFSLEMPSEQIMMRSLASL
                     SRVDQTKIRTGQLDDEDWARISGTMGILLEKRNIYIDDSSGLTPTEVRSRARRIAREH
                     GGIGLIMIDYLQLMRVPALSDNRTLEIAEISRSLKALAKELNVPVVALSQLNRSLEQR
                     ADKRPVNSDLRESGSIEQDADLIMFIYRDEVYHENSDLKGIAEIIIGKQRNGPIGTVR
                     LTFNGQWSRFDNYAGPQYDDE"
     gene            10521..11504
                     /locus_tag="OO357_001431"
     CDS             10521..11504
                     /locus_tag="OO357_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HCR5886429.1"
                     /translation="MATRIEFHKHGGPEVLQAVEFTPADPAENEIQVENKAIGINFID
                     TYIRSGLYPPPSLPSGLGTEAAGIVSKVGSGVKHIKAGDHVVYAQSALGAYSSVHNIN
                     ADKAAILPAAISFEQAAASFLKGLTVYYLLRKTYEIKPDEQFLFHAAAGGIGLIACQW
                     AKALGAKLIGTVGTAQKAQSALKAGAWQVINYREENLVERLKEITGGKKVRVVYDSVG
                     RDTWERSLDCLQRRGLMVSFGNSSGAVTGVNLGILNQKGSLYVTRPSLQGYITTREEL
                     TEASNELFSLIASGVIKVDVAEQQKYPLKDAQRAHEILESRATQGSSLLIP"
     gene            complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OO357_001432"
     CDS             complement(11670..11912)
                     /gene="pspG"
                     /locus_tag="OO357_001432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709870.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope stress response protein PspG"
                     /protein_id="HCR5886430.1"
                     /translation="MLELLFVIGFFVMLMVTGVSLLGIIAALVVATAIMFLGGMLALM
                     IKLLPWLLLAIAVVWVIKAIKAPKMPKYQRYDRWRY"
     gene            complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OO357_001433"
     CDS             complement(12046..13083)
                     /gene="dusA"
                     /locus_tag="OO357_001433"
                     /EC_number="1.3.1.-"
                     /EC_number="1.3.1.91"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine(20/20a) synthase DusA"
                     /protein_id="HCR5886431.1"
                     /translation="MHGNSEMQKINQTSAMPEKTDVHWSGRFSVAPMLDWTDRHCRYF
                     LRLLSRNTLLYTEMVTTGAIIHGKGDYLAYSEEEHPVALQLGGSDPAALAQCAKLAEA
                     RGYDEINLNVGCPSDRVQNGMFGACLMGNAQLVADCVKAMRDVVSIPVTVKTRIGIDD
                     QDSYEFLCDFINTVSGKGECEMFIIHARKAWLSGLSPKENREIPPLDYPRVYQLKRDF
                     PHLTMSINGGIKSLEEAKAHLQHMDGVMVGREAYQNPGILAAVDREIFGSSDTDADPV
                     AVVRAMYPYIEHELSQGTYLGHIIRHMLGLFQGIPGARQWRRYLSENAHKAGADINVL
                     EHALKLVADKR"
     gene            complement(13446..14431)
                     /locus_tag="OO357_001434"
                     /pseudo
     CDS             complement(13446..14431)
                     /locus_tag="OO357_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001560258.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2713 domain-containing protein"
     gene            14749..15264
                     /gene="zur"
                     /locus_tag="OO357_001435"
     CDS             14749..15264
                     /gene="zur"
                     /locus_tag="OO357_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc uptake transcriptional repressor Zur"
                     /protein_id="HCR5886432.1"
                     /translation="MEKTTTQELLAQAEKICAQRNVRLTPQRLEVLRLMSLQDGAISA
                     YDLLDLLREAEPQAKPPTVYRALDFLLEQGFVHKVESTNSYVLCHLFDQPTHTSAMFI
                     CDRCGAVKEECAEGVEDIMHTLAAKMGFALRHNVIEAHGLCAACVEVEACRHPEQCQH
                     DHSVQVKKKPR"
     gene            complement(15306..15515)
                     /locus_tag="OO357_001436"
     CDS             complement(15306..15515)
                     /locus_tag="OO357_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HCR5886433.1"
                     /translation="MNKDEAGGNWKQFKGKVKEQWGKLTDDDMTIIEGKRDQLVGKIQ
                     ERYGYQKDQAEKEVVDWETRNEYRW"
     gene            complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OO357_001437"
     CDS             complement(15631..16956)
                     /gene="dinF"
                     /locus_tag="OO357_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter DinF"
                     /protein_id="HCR5886434.1"
                     /translation="MAFLTSSDKALWHLALPMIFSNITVPLLGLVDTAVIGHLDSPVY
                     LGGVAVGATATSFLFMLLLFLRMSTTGLTAQAYGAKNPQALARALVQPLLLALGAGAL
                     IALLRTPIIDLALHIVGGSEAVLEQARRFLEIRWLSAPASLANLVLLGWLLGVQYARA
                     PVILLVVGNILNIVLDVWLVMGLHMNVQGAALATVIAEYATLLIGLLMVRKILKLRGI
                     SGEMLKTAWRGNFRRLLALNRDIMLRSLLLQLCFGAITVLGARLGSDIIAVNAVLMTL
                     LTFTAYALDGFAYAVEAHSGQAYGARDGSQLLDVWRAACRQSGIVALLFSVVYLLAGE
                     HIIALLTSLTQIQQLADRYLIWQVILPLVGVWCYLLDGMFIGATRAAEMRNSMAVAAA
                     GFALTLLTLPWLGNHGLWLALTVFLALRGLSLAAIWRRHWRNGTWFAAT"
     gene            complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OO357_001438"
     CDS             complement(17029..17637)
                     /gene="lexA"
                     /locus_tag="OO357_001438"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000646075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repressor LexA"
                     /protein_id="HCR5886435.1"
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAE
                     EHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDP
                     SLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKK
                     QGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
     gene            complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OO357_001439"
     CDS             complement(17747..18115)
                     /gene="dgkA"
                     /locus_tag="OO357_001439"
                     /EC_number="2.7.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HCR5886436.1"
                     /translation="MANNTTGFTRIIKAAGYSWKGLRAAWINEAAFRQEGVAVLLAVV
                     IACWLDVDAITRVLLISSVMLVMIVEILNSAIEAVVDRIGSEYHELSGRAKDMGSAAV
                     LIAIIVAVITWCILLWSHFG"
     gene            18286..20709
                     /gene="plsB"
                     /locus_tag="OO357_001440"
     CDS             18286..20709
                     /gene="plsB"
                     /locus_tag="OO357_001440"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsB"
                     /protein_id="HCR5886437.1"
                     /translation="MSGWPRIYYKLLNLPLSILVKSKSIPADPAPELGLDTSRPIMYV
                     LPYNSKADLLTLRAQCLAHDLPDPLEPLEIDGTLLPRYVFIHGGPRVFTYYTPKEESI
                     KLFHDYLDLHRSNPNLDVQMVPVSVMFGRAPGREKGEVNPPLRMLNGVQKFFAVLWLG
                     RDSFVRFSPSVSLRRMADEHGTDKTIAQKLARVARMHFARQRLAAVGPRLPARQDLFN
                     KLLASRAIAKAVEDEARSKKISHEKAQQNAIALMEEIAANFSYEMIRLTDRILGFTWN
                     RFYQGINVHNAERVRQLAHDGHELVYVPCHRSHMDYLLLSYVLYHQGLVPPHIAAGIN
                     LNFWPAGPIFRRLGAFFIRRTFKGNKLYSTVFREYLGELFSRGYSVEYFVEGGRSRTG
                     RLLDPKTGTLSMTIQAMLRGGTRPITLIPIYIGYEHVMEVGTYAKELRGATKEKESLP
                     QMLRGLSKLRNLGQGYVNFGEPMPLMTYLNQHVPDWRESIDPIEAVRPAWLTPTVNNI
                     AADLMVRINNAGAANAMNLCCTALLASRQRSLTREQLTEQLNCYLDLMRNVPYSTDST
                     VPSASASELIDHALQMNKFEVEKDTIGDIIILPREQAVLMTYYRNNIAHMLVLPSLMA
                     AIVTQHRHISRDVLMEHVNVLYPMLKAELFLRWDRDELPDVIDALANEMQRQGLITLQ
                     DDELHINPAHSRTLQLLAAGARETLQRYAITFWLLSANPSINRGTLEKESRTVAQRLS
                     VLHGINAPEFFDKAVFSSLVLTLRDEGYISDSGDAEPAETMKVYQLLAELITSDVRLT
                     IESATQGEG"
     gene            complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OO357_001441"
     CDS             complement(20864..21736)
                     /gene="ubiA"
                     /locus_tag="OO357_001441"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HCR5886438.1"
                     /translation="MEWSLTQNKLLAFHRLMRTDKPIGALLLLWPTLWALWVATPGVP
                     QLWIMAVFVAGVWLMRAAGCVVNDYADRKFDGHVKRTANRPLPSGAVTEKEARALFVV
                     LVLISFLLVLTLNTMTILLSIAALALAWVYPFMKRYTHLPQVVLGAAFGWSIPMAFAA
                     VSESVPLSCWLMFLANILWAVAYDTQYAMVDRDDDVKIGIKSTAILFGQYDKLIIGIL
                     QIGVLALMAIIGELNGLGWGYYWSIVVAGALFVYQQKLIANREREACFKAFMNNNYVG
                     LVLFLGLAMSYWHF"
     gene            complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OO357_001442"
     CDS             complement(21749..22246)
                     /gene="ubiC"
                     /locus_tag="OO357_001442"
                     /EC_number="4.1.3.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate lyase"
                     /protein_id="HCR5886439.1"
                     /translation="MSHPALTQLRALRYFKEIPALDPQLLDWLLLEDSMTKRFEQQGK
                     TVSVTMIREGFVEQNEIPEELPLLPKESRYWLREILLCADGEPWLAGRTVVPVSTLSG
                     PELALQKLGKTPLGRYLFTSSTLTRDFIEIGRDAGLWGRRSRLRLSGKPLLLTELFLP
                     ASPLY"
     gene            complement(22469..24049)
                     /locus_tag="OO357_001443"
                     /pseudo
     CDS             complement(22469..24049)
                     /locus_tag="OO357_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313048.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SopA family protein"
     gene            complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OO357_001444"
     CDS             complement(24278..25198)
                     /gene="malM"
                     /locus_tag="OO357_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose operon protein MalM"
                     /protein_id="HCR5886440.1"
                     /translation="MKMNKSLIALCLSAGLLASAPGISLADVNYVPQNTSDAPAIPSA
                     ALQQLTWTPVDQSKTQTTQLATGGQQLNVPGISGPVAAYSVPANIGELTLTLTSEVNK
                     QTSVFAPNVLILDQNMTPSAFFPSSYFTYQELGVMSADRLEGVMRLTPALGQQKLYVL
                     VFTTEKDLQQTTQLLDPAKAYAKGVGNSIPDIPDPVARHTSDGLLKLKVKTNSSSSVL
                     VGPLFGSSAPAPVTVGNTAAPAVAAPAPVPVKKSEPMLNDTESYFNTAIKNAVAKGDV
                     DKALKLLDEAERLGSTSARSTFISSVKGKG"
     gene            complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OO357_001445"
     CDS             complement(25350..26690)
                     /gene="lamB"
                     /locus_tag="OO357_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltoporin LamB"
                     /protein_id="HCR5886441.1"
                     /translation="MMITLRKLPLAVAVAAGVMSAQAMAVDFHGYARSGIGWTGSGGE
                     QQCFQTTGAQSKYRLGNECETYAELKLGQEVWKEGDKSFYFDTNVAYSVAQQNDWEAT
                     DPAFREANVQGKNLIEWLPGSTIWAGKRFYQRHDVHMIDFYYWDISGPGAGLENIDVG
                     FGKLSLAATRSSEAGGSSSFASNNIYDYTNETANDVFDVRLAQMEINPGGTLELGVDY
                     GRANLRDNYRLVDGASKDGWLFTAEHTQSVLKGFNKFVVQYATDSMTSQGKGLSQGSG
                     VAFDNEKFAYNINNNGHMLRILDHGAISMGDNWDMMYVGMYQDINWDNDNGTKWWTVG
                     IRPMYKWTPIMSTVMEIGYDNVESQRTGDKNNQYKITLAQQWQAGDSIWSRPAIRVFA
                     TYAKWDEKWGYDYNGDSKVNPNYGKAVPADFNGGSFGRGDSDEWTFGAQMEIWW"
     gene            complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OO357_001446"
     CDS             complement(26762..27877)
                     /gene="malK"
                     /locus_tag="OO357_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011915502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter ATP-binding
                     protein MalK"
                     /protein_id="HCR5886442.1"
                     /translation="MASVQLQNVTKAWGEVVVSKDINLDIHEGEFVVFVGPSGCGKST
                     LLRMIAGLETITSGDLFIGEKRMNDTPPAERGVGMVFQSYALYPHLSVAENMSFGLKL
                     AGAKKEVINQRVNQVAEVLQLAHLLDRKPKALSGGQRQRVAIGRTLVAEPSVFLLDEP
                     LSNLDAALRVQMRIEISRLHKRLGRTMIYVTHDQVEAMTLADKIVVLDAGRVAQVGKP
                     LELYHYPADRFVAGFIGSPKMNFLPVKVTATAIDQVQVELPMPNRQQVWLPVESRDVQ
                     VGANMSLGIRPEHLLPSDIADVILEGEVQVVEQLGNETQIYIQIPSIRQNLVYRQNDV
                     VLVEEGATFAIGLPPERCHLFREDGTACRRLHKEPGV"
     gene            28242..29432
                     /gene="malE"
                     /locus_tag="OO357_001447"
     CDS             28242..29432
                     /gene="malE"
                     /locus_tag="OO357_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000695396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose/maltodextrin ABC transporter
                     substrate-binding protein MalE"
                     /protein_id="HCR5886443.1"
                     /translation="MKIKTGARILALSALTTMMFSASALAKIEEGKLVIWINGDKGYN
                     GLAEVGKKFEKDTGIKVTVEHPDKLEEKFPQVAATGDGPDIIFWAHDRFGGYAQSGLL
                     AEITPDKAFQDKLYPFTWDAVRYNGKLIAYPIAVEALSLIYNKDLLPNPPKTWEEIPA
                     LDKELKAKGKSALMFNLQEPYFTWPLIAADGGYAFKYENGKYDIKDVGVDNAGAKAGL
                     TFLVDLIKNKHMNADTDYSIAEAAFNKGETAMTINGPWAWSNIDTSKVNYGVTVLPTF
                     KGQPSKPFVGVLSTGINAASPNKELAKEFLENYLLTDEGLEAVNKDKPLGAVALKSYE
                     EELAKDPRIAATMENAQKGEIMPNIPQMSAFWYAVRTAVINAASGRQTVDEALKDAQT
                     RITK"
     gene            29586..31130
                     /gene="malF"
                     /locus_tag="OO357_001448"
     CDS             29586..31130
                     /gene="malF"
                     /locus_tag="OO357_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalF"
                     /protein_id="HCR5886444.1"
                     /translation="MDVIKKKHWWQSDALKWSVLGLLGLLVGYLVVLMYAQGEYLFAI
                     TTLILSSAGLYIFANRKAYAWRYVYPGMAGMGLFVLFPLVCTIAIAFTNYSSTNQLTF
                     ERAQEVLLDRSWQAGKTYNFGLYPAGDEWQLALSDGETGKNYLSDAFKFGGEQKLQLK
                     ETTAQPEGERANLRVITQNRQALSDITAILPDGNKVMMSSLRQFSGTQPLYTLDGDGT
                     LTNNQSGVKYRPNNQIGFYQSITADGNWGDEKLSPGYTVTTGWKNFTRVFTDEGIQKP
                     FLAIFVWTVVFSLITVFLTVAVGMVLACLVQWEALRGKAVYRVLLILPYAVPSFISIL
                     IFKGLFNQSFGEINMMLSALFGVKPAWFSDPTTARTMLIIVNTWLGYPYMMILCMGLL
                     KAIPDDLYEASAMDGAGPFQNFFKITLPLLIKPLTPLMIASFAFNFNNFVLIQLLTNG
                     GPDRLGTTTPAGYTDLLVNYTYRIAFEGGGGQDFGLAAAIATLIFLLVGALAIVNLKA
                     TRMKFD"
     gene            31145..32035
                     /gene="malG"
                     /locus_tag="OO357_001449"
     CDS             31145..32035
                     /gene="malG"
                     /locus_tag="OO357_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001252048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose ABC transporter permease MalG"
                     /protein_id="HCR5886445.1"
                     /translation="MAMVQPKSQKARLFITHLLLLLFIAAIMFPLLMVVAISLRQGNF
                     ATGSLIPEQISWDHWKLALGFSVEQADGRITPPPFPVLLWLWNSVKVAGISAIGIVAL
                     STTCAYAFARMRFPGKATLLKGMLIFQMFPAVLSLVALYALFDRLGEYIPFIGLNTHG
                     GVIFAYLGGIALHVWTIKGYFETIDSSLEEAAALDGATPWQAFRLVLLPLSVPILAVV
                     FILSFIAAITEVPVASLLLRDVNSYTLAVGMQQYLNPQNYLWGDFAAAAVMSALPITI
                     VFLLAQRWLVNGLTAGGVKG"
     gene            32406..33881
                     /gene="xylE"
                     /locus_tag="OO357_001450"
     CDS             32406..33881
                     /gene="xylE"
                     /locus_tag="OO357_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose transporter XylE"
                     /protein_id="HCR5886446.1"
                     /translation="MNTQYNSSYIFSITLVATLGGLLFGYDTAVISGTVESLNTVFVA
                     PQNLSESAANSLLGFCVASALIGCIIGGALGGYCSNRFGRRDSLKISAVLFFISGVGS
                     AWPELGFTSINPDNTVPIYLAGYVPEFVIYRIIGGIGVGLASMLSPMYIAELAPAHIR
                     GKLVSFNQFAIIFGQLLVYCVNYFIARSGDASWLNTDGRRYMFASECIPALLFLMLLY
                     TVPESPRWLMSRGKQEQTESILRKIMGNTLATQAVQEIKHSLDHGRKTGGRLLMFGVG
                     VIVIGVMLSIFQQFVGINVVLYYAPEVFKTLGASTDIALLQTIIVGVINLTFTVLAIM
                     TVDKFGRKPLQIIGALGMAIGMFSLGTAFYTQASGIVALLSMLFYVAAFAMSWGPVCW
                     VLLSEIFPNAIRGKALAIAVAAQWLANYFVSWTFPMMDKNSWLVAHFHNGFSYWIYGC
                     MGVLAALFMWKFVPETKGKTLEELEALWEPETKKTQQTATL"
     gene            complement(33925..34335)
                     /gene="psiE"
                     /locus_tag="OO357_001451"
     CDS             complement(33925..34335)
                     /gene="psiE"
                     /locus_tag="OO357_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate-starvation-inducible protein PsiE"
                     /protein_id="HCR5886447.1"
                     /translation="MTSLSRPRVEFISTILQTVLNLGLLCLGLILVVFLGKETVHLAD
                     VLFAPEQTSKYELVEGLVVYFLYFEFIALIVKYFQSGFHFPLRYFVYIGITAIVRLII
                     VDHKSPLDVLIYSAAILLLVITLWLCNSKRLKRE"
     gene            complement(34461..34604)
                     /locus_tag="OO357_001452"
     CDS             complement(34461..34604)
                     /locus_tag="OO357_001452"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886448.1"
                     /translation="MFQRNIVDFSRIAGGWESPEWVIFELPALVRFVLPDAPPGAAYP
                     AYG"
     gene            34605..34883
                     /locus_tag="OO357_001453"
     CDS             34605..34883
                     /locus_tag="OO357_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886449.1"
                     /translation="MKRNLIKVVKMKPYFAALMLSVSVLPAYAGPLGTADKADLPQSN
                     VSSPMMAQSLRQPDLQPISTDRKTECFRLYTPDRKPGVNCVPDGSTGH"
     gene            complement(34930..37026)
                     /locus_tag="OO357_001454"
     CDS             complement(34930..37026)
                     /locus_tag="OO357_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5886450.1"
                     /translation="MKKRHLLSLLALGISTACYGETYPAPIGPSQSDFGGVGLLQTPT
                     ARMAREGELSLNYRDNDQYRYYSASVQLFPWLETTLRYTDVRTRQYSSVEAFSGDQTY
                     KDKAFDLKLRLWEESYWLPQVAVGARDIGGTGLFDAEYLVASKAWGPFDFTLGLGWGY
                     LGTSGNVKNPLCSASDKYCYRDNSYKQAGSIDGSQMFHGPASLFGGVEYQTPWQPLRL
                     KLEYEGNNYQQDFAGKLEQKSKFNVGAIYRVTDWADVNLSYERGNTFMFGVTLRTNFN
                     DLRPSYIDNARPQYQPQPQDAILQHSVVANQLTLLKYNAGLADPQIQAKGDTLYVTGE
                     QVKYRDSREGIIRANRIVMNDLPDGIKTIRITENRLNMPQVTTETDVASLKNHLAGEP
                     LGHETTLAHKRVEPVVPKSTEQGWYIDKSRFDFHIDPVLNQSVGGPENFYMYQLGVMG
                     TADLWLTDHLLTTGSLFANLANNYDKFNYTNPPQDSHLPRVRTHVREYVQNDVYVNNL
                     QANYFQHLGNGFYGQVYGGYLETMFGGAGAEVLYRPLDSNWAFGLDANYVKQRDWRSA
                     KDMMKFTDYSVKTGHLTAYWTPSFAQDVLVKASVGQYLAGDKGGTLEIAKRFDSGVVV
                     GGYATITNVSKEEYGEGDFTKGVYVSVPLDLFSSGPTRSRAAISWTPLTRDGGQQLGR
                     KFQLYDMTSDRSVNFR"
     gene            complement(37026..37763)
                     /locus_tag="OO357_001455"
     CDS             complement(37026..37763)
                     /locus_tag="OO357_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5886451.1"
                     /translation="MIKQTIVALLLSVGASSVFAAGTVKVFSNGSSEAKTLTGAEHLI
                     DLVGQPRLANSWWPGAVISEELATAAALRQQQALLTRLAEQGADSSTDDAAAINALRQ
                     QIQALKVTGRQKINLDPDIVRVAERGNPPLQGNYTLWVGPPPSTVTLFGLISHPGNQP
                     FTPGRDVASYLSDQSLLSGADRSYAWVVYPDGRTQKAPVAYWNKRHVEPMPGSIIYVG
                     LADSVWSETPDALNADILQTLTQRIPQ"
     gene            complement(37760..38398)
                     /locus_tag="OO357_001456"
     CDS             complement(37760..38398)
                     /locus_tag="OO357_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418451.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5886452.1"
                     /translation="MKRPALILICLLLQACSATTKELGNSLWDSLFGTPGVQLTDDDI
                     QNMPYASQYMQLNGGPQLFVVLAFAEDGQQKWVTQDQATLVTQHGRLVKTLLGGDNLI
                     EVNNLAADPLIKPAQIVDGATWTRTMGWTEYQQVRYATARSVFKWDGTDTVKVGSDET
                     LVRVLDEEVSTDQARWHNRYWIDSEGQIRQSEQYLGADYFPVKTTLIKAAKQ"
     gene            complement(38512..38754)
                     /locus_tag="OO357_001457"
     CDS             complement(38512..38754)
                     /locus_tag="OO357_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886453.1"
                     /translation="MKKVLYGIFAISALAATSAWAAPVQVGEAAESAATSVSAGSSSA
                     TSVSTVSSAVGVALAATGGGDGSNTGTTTTTTTSTQ"
     gene            complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="OO357_001458"
     CDS             complement(39217..40866)
                     /gene="pgi"
                     /locus_tag="OO357_001458"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HCR5886454.1"
                     /translation="MKNINPTQTAAWQALQKHFDEMKDVTIADLFAKDGDRFSKFSAT
                     FDDQMLVDYSKNRITEETLAKLQDLAKECDLAGAIKSMFSGEKINRTENRAVLHVALR
                     NRSNTPILVDGKDVMPEVNAVLEKMKTFSEAIISGEWKGYTGKAITDVVNIGIGGSDL
                     GPYMVTEALRPYKNHLNMHFVSNVDGTHISEVLKKVNPETTLFLVASKTFTTQETMTN
                     AHSARDWFLKAAGDEKHVAKHFAALSTNAKAVGEFGIDTANMFEFWDWVGGRYSLWSA
                     IGLSIVLSIGFDNFVELLSGAHAMDKHFSTTPAEKNLPVLLALIGIWYNNFFGAETEA
                     ILPYDQYMHRFAAYFQQGNMESNGKYVDRNGNVVDYQTGPIIWGEPGTNGQHAFYQLI
                     HQGTKMVPCDFIAPAITHNPLSDHHQKLLSNFFAQTEALAFGKSREVVEQEYRDQGKD
                     PATLDYVVPFKVFEGNRPTNSILLREITPFSLGALIALYEHKIFTQGVILNIFTFDQW
                     GVELGKQLANRILPELKDDKEISSHDSSTNGLINRYKAWRG"
     gene            41391..>42657
                     /gene="lysC"
                     /locus_tag="OO357_001459"
     CDS             41391..>42657
                     /gene="lysC"
                     /locus_tag="OO357_001459"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-sensitive aspartokinase 3"
                     /protein_id="HCR5886455.1"
                     /translation="MSEIVVSKFGGTSVADFDAMNRSADIVLSDANVRLVVLSASAGI
                     TNLLVALAEGLEPGERFEKLDAIRNIQFAILERLRYPNVIREEIERLLENITVLAEAA
                     ALATSPALTDELVSHGELMSTLLFVEILRERDVQAQWFDVRKVMRTNDRFGRAEPDVA
                     ALAELAALQLLPRLNEGLVITQGFIGSENKGRTTTLGRGGSDYTAALLAEALHASRVD
                     IWTDVPGIYTTDPRVVSAAKRIDEIAFAEAAEMATFGAKVLHPATLLPAVRSDIPVFV
                     GSSKDPRAGGTLVCNKTENPPLFRALALRRNQTLLTLHSLNMLHSRGFLAEVFGILAR
                     HNISVDLITTSEVSVALTLDTTGSTSTGDTLLTQSLLMELSALCRVEVEEGLALVALI
                     GNDLSKACGVGKEVFGVLEPFNIRMICYGA"
BASE COUNT        10613 a        10986 c        10468 g        10590 t
ORIGIN      
        1 cctccataac ggaggcttct tttttatggc tggcagatgc tttaatcatc caccttaaaa
       61 caatataacc tattgtttta atgacaaatc agaacggaat gtcatcatca aagtccatcg
      121 gcggctcgtt agacggcgct gccggagcgg actgctgcgg gcgagactgc gcgccgccgc
      181 tgaactgatt gccaccctgc ggctgctgag gctgacccca accgccctgc ggctgaccac
      241 caccgatatt gccacctgcc ggagcgccac caccctgacg accacccagc atctgcatgg
      301 tgccgccaac gttcaccacg acttctgtgg tgtagcgatc ctgaccggat tgatcggtcc
      361 atttacgggt acgcagctga ccttcgatat aaacctgaga acctttacgc agatattcgc
      421 tcgccacttc tgccagtttg ccgaacagca caacgcggtg ccattcagtc tgttctttca
      481 tctcgccggt cgctttatca cgccaggatt cggaagtagc cagcgtaatg ttggcaactg
      541 cgccaccatt tggcatgtag cgtacttccg ggtcctgacc cagattacca acgagaataa
      601 ccttgtttac gcctctgctg gccatgttcg tgtctcctga aaaaaatcgt tctgaataag
      661 tgtaaacgcg cgattgtacc attaccaata gcgcttttac tatgttgtga cctcggttcc
      721 gggaaacaaa cctggccaga cattgttaca caacactccg gataatgcat tccaatactg
      781 tatattcatt caggtcaatt tgtgtcataa ttaaccgttt gtgatcgccg gtagcaccat
      841 gccaccgggc aaaaaagcgt ttaatccggg aaaggtgaat ggataagatc gaagttcggg
      901 gcgcccgcac ccataatctc aaaaacatca acctcgttat cccccgcgac aagctcattg
      961 tcgtgaccgg gctttcgggt tctggcaaat cctcgctcgc tttcgacacc ttatatgccg
     1021 aagggcagcg ccgttacgtt gaatcccttt ccgcctacgc gcggcagttt ctgtcactga
     1081 tggaaaagcc ggacgtcgat catattgagg ggctttctcc tgccatctca attgagcaga
     1141 aatcgacgtc tcataacccg cgttctacgg tggggacaat caccgaaatc cacgactatt
     1201 tgcgtttgtt gttcgcccgc gtcggcgaac cgcgctgtcc ggaccacgac gtcccgctgg
     1261 cggcgcaaac cgtcagccag atggtggata acgtgctgtc gcagccggaa ggcaagcgtc
     1321 tgatgctgct cgcgccaatc attaaagagc gcaaaggcga acacaccaaa acgctggaga
     1381 acctggcaag ccagggttac atccgtgctc gtattgatgg cgaagtctgc gatctttccg
     1441 atccgccgaa actggaactg caaaagaaac ataccattga agtggtggtt gatcgcttca
     1501 aggtgcgtga cgatcttacc caacgtcttg cggagtcgtt tgaaaccgcg ctggaacttt
     1561 ccggtggtac agcggtagtg gcggatatgg acgacccgaa agcggaagag ctgctgttct
     1621 ccgccaactt cgcctgccca atttgcggct acagtatgcg tgaactggag ccgcgactgt
     1681 tttcgtttaa caacccggca ggtgcctgcc cgacctgtga cggccttggc gtacagcaat
     1741 atttcgatcc tgaccgcgtg atccaaaacc ccgagctgtc actggctggc ggtgcgatcc
     1801 gtggctggga tcgccgcaac ttctattact tccagatgct gaaatcgctg gcagatcact
     1861 ataagttcga cgtcgaagcg ccgtggggca gcctgagcgc gaacgtgcat aaagtggtgt
     1921 tgtacggttc tggcaaagaa aacattgaat tcaaatacat gaacgatcgt ggcgatacct
     1981 ccatccgtcg tcatccgttc gaaggcgtgc tgcacaatat ggagcgccgt tataaagaga
     2041 cagaatccag tgcggtacgt gaagaattag ccaagtttat cagcaatcgc ccatgcgcca
     2101 gctgcgaagg gacgcgtctg cgtcgggaag cgcgccacgt ttatgtcgag aatacgccgc
     2161 tgcctgctat ctccgacatg agcatcggtc atgcgatgga attcttcaac aatctcaaac
     2221 tcgctggtca gcgggcgaag attgcggaaa aaattcttaa agagatcggc gatcgcctga
     2281 aattcctcgt aaacgtcggc ctgaattacc tgacactttc ccgctcggca gaaacacttt
     2341 ccggcggtga agcccagcgt atccgtctgg cgagccagat tggtgcgggc ctggttggcg
     2401 ttatgtacgt gctggacgag ccgtctatcg gcctgcacca gcgcgataac gagcgcctgt
     2461 tgggtacgct tatccatctg cgcgatctcg gtaataccgt gattgtggtg gagcatgacg
     2521 aagacgcaat tcgcgccgct gaccatgtga tcgatatcgg tcctggtgcg ggtgttcacg
     2581 gcggtgaagt ggtcgcagaa ggtccgctgg aagcgattat ggcggtgcct gaatcgttga
     2641 ccgggcagta catgagcggt aaacgcaaga ttgaagtgcc gaagaaacgc gttccggcga
     2701 atccagaaaa agtgctgaag ctgacaggcg cacgcggcaa caacctgaag gacgtgacgc
     2761 tcacgctgcc agtcggtctg tttacctgca tcacaggggt ttcaggttcc ggtaaatcga
     2821 cgctgattaa cgacacactg ttcccgattg cccaacgcca gttgaatggt gcgaccatcg
     2881 ccgaaccggc accgtatcgc gatattcagg gactggagca tttcgataaa gtgatcgata
     2941 tcgaccaaag cccaattggt cgtactccac gttctaaccc ggcgacctat accggcgtgt
     3001 ttacgcctgt gcgcgaactg tttgcgggcg taccggaatc ccgtgcgcgt ggttatacgc
     3061 caggacgttt cagctttaac gtccgtggcg ggcgctgcga agcctgtcag ggcgacggtg
     3121 tgatcaaagt ggagatgcac ttcctgccgg atatctacgt gccgtgcgat cagtgcaaag
     3181 gtaaacgcta taaccgtgaa acgctggaaa ttaagtacaa aggcaaaacc atccacgaag
     3241 tgctggatat gaccatcgaa gaggcgcgtg agttctttga tgcggtgcca gctctggcgc
     3301 gtaagctgca aacgttgatg gacgttggtc tgacgtacat tcgcctgggg cagtccgcaa
     3361 ccacactttc tggtggtgaa gcccagcgcg tgaagctggc gcgtgagctg tcaaaacgcg
     3421 gcaccgggca gacgctgtat attctcgacg agccgaccac cggtctgcac ttcgccgata
     3481 ttcagcaact cctcgacgtg ctgcataaac tgcgcgatca gggcaatacc attgtggtga
     3541 ttgagcacaa tctcgacgtg atcaaaaccg ctgactggat tgtcgacctg ggaccagaag
     3601 gcggcagtgg cggcggcgaa atcctcgtct ccggtacgcc agaaaccgtc gcggagtgcg
     3661 aagcttcgca tacggcacgc ttcctcaagc cgatgctgta atcgttaagg ccgctttctg
     3721 agcggccttt tcctttcaga gttgtaccag caatttacgc ttttcttccg gtagtaaatt
     3781 cactgcctgc tgataagacg catccaccag ataatagatt tgcgaatccg gcagcgaacc
     3841 gtcgagatac acggtgctcc agtgcgcttt attcagatgg cggctaggac gcacatcgct
     3901 gtgctgctga cgtagcagct ccgccagctc cgggctggtt ttcagcgaaa cagctgggcg
     3961 attttctact tctttcacca tcgcaaacag tacatcttcc actttgatct gcgtcgcttt
     4021 ccagtcgtta tgcacgctct gttctgcgct tggttttgcc atgcaatatt gtagcaactc
     4081 cgaaatggtc attttttact ccccttgtag tgtcgcgatg atgcgacgcg atccgccgtg
     4141 gatgcgatgt tcccccagcc agatgccttg ccaagtgccg gtctgaatac gccctttatg
     4201 taccggcaat acaagcgatg ttcccagcat tgaggatttg atatgagaag gcatatcgtc
     4261 tgctccctca tagtcatgct cataatttcc gttgtcggga acggtgcgga ggaaaaaaca
     4321 ctccatgtcg tggcgtacgg tgggatcgca gttctcatta agtgtcagag aggcggaggt
     4381 atgttgcagc aacagatgca gtaagccgat gttaacgcgc ggcatatcag ccagctgatt
     4441 cagaatttca tccgttacca gatgaaaccc acgagatttg gcgctaagcg tgagcgtctt
     4501 ttgataccac atgtgctgct ccttgataaa actctcttaa tcagtttgca gcaagacagc
     4561 gaaaggataa aggtgtgatt aaaaaaacag cattcgggag agcatcactc tctcccgctc
     4621 tgtcagtatt ctgaattgac gatcacctct tcaccaaacg cacccgcttg tggcaagggt
     4681 ttgtaggtag agttggaggc gcgcagaatg cggataccac gagcgccgac atcgcgtgcg
     4741 gcggtaatat cgttatcgga atcgccataa aaaattcgga tatttttatc ctgcagccat
     4801 tgcgattttg tattttgccc tggtttatcg cccgcaaaga ttaccggatt catgttggtg
     4861 gcaggaatat gaaaattatc cgccagcgtt tttgaaaccg tttctgtttt cgtcgggcta
     4921 cgaccagtca caaagaagat cgcgtcaccg cggcgtacat gcatatcaat cagctggcga
     4981 gcgacctctt ttggaatgct gaattcatcc cagccattat tcattttttc ccagaacaca
     5041 ggatttttca gataatcttc gctttctggc gagaaggttt ttttgccgcg ccagaacccc
     5101 ggactggaaa aaagtaccgt gtcatcgata tcaaacccca ccgccattgg cggacgccct
     5161 gcgaggctat tttcaatttg tgcgaccgaa acccaatgaa ttggtgcctg ttcagcaagc
     5221 ctggcaacgt tagtcccagg gttaagcggt gaaggagatg aggccagggc aacagcggaa
     5281 ctgtttagag cgaacaataa gcaaacggca ctgattgcct gtgtgatctt gcgcatattt
     5341 ttccctaaat agtcagtatg ttgaaacttt ttattgtgag atttgttgca aaaaccatct
     5401 gaccataacg ccagctgccg gggaaaggaa ggattatctg cggtttttgt gatgaaaagc
     5461 aggatgatta tatttaaccc agtaaatacg ttcctgtctt tttttgtata aaaaatgttt
     5521 attactcgat gtaaggatac attaatttta cgtttattat gatgaatcct cggcaaatga
     5581 gcgaaatttg atagaacatt tatctgctgt acgatttaat gaacttaatg atacagcatt
     5641 acaaaaacaa tcgaagttta taaagatgat ttctgattga ccagccccct accctacaat
     5701 ggtgattaat tttattactc gcgatggaga tgatgatatg aatatatcat atgtgaatag
     5761 caacaaaacg acatctttac ctgttgagct agatgcacta aataataaag atatctctta
     5821 tgccaaagat ttctttttat acatagaaac acagttaaaa attgcaaaag atttttgtag
     5881 acctggagaa gaagtatcaa gttctattgc aagtaaagtt tttcacgcat ttattgattt
     5941 agttaacaaa atcaggggta agaaagactt tatgtatatt tgcacgcttt gctgttttgc
     6001 cgaggaggtt aaaggtgatt attctcatta caggaccttt ttatttgata ttggtaatca
     6061 atataaggtt aaacttacac aaagcggaaa aaagagttct ctttaacatt agaatttaac
     6121 gatactataa ttgaatctca gaaagtcaca ggcaataagg caaagcatat tcttgaggat
     6181 atagaaatat tctatcgtaa caaacccgat acttattatt aaatttaata atggttagat
     6241 gtgcagaaat tattactcca cacgaaaaaa tagggaaagg aattccagat aattgggata
     6301 aacttaacaa taaaagaact gacaacgttt tattatctct cacgtagaac aacggcatca
     6361 aaaaaatcat ctacaaagaa atagtgaaag cgacgacagt ttaatttcac tgcaggctgg
     6421 gtaactccag cctgctttcc tgcattacat caccgcagca aacgcctttg ccacacgttg
     6481 tacatttgcc gtatttaacc cggcgacaca catgcgaccg ctggcgatga gatagacacc
     6541 aaattcttca cgtagtcggt caacctgagc ggcacttaaa ccggtataac tgaacatgcc
     6601 gcgctgatta agcagataat cgaaattgcg ttctggcatc tctgtgctta ataccttcac
     6661 caattcctga cgcattgcca gaatgcgagt acgcatctct tctacttccg ccagccagct
     6721 ggctttcaat gcctcgtcat tcagcactgc agccaccacc tgcgcaccaa aattcggcgg
     6781 gctggagtag ttgcggcgaa ctgttgcttt caattgcccc agtacgcggc cagcggcttc
     6841 ggcatcttca cacagaacag aaagtccgcc gacgcgctcg ccgtaaaggg agaaaatttt
     6901 cgagaacgaa ttgctcacca gagcgggtaa tccagcactg gcaatggcgc gaatagcgta
     6961 ggcatcctct tccataccgg cactaaatcc ttgataggca atatcgagga atgggataag
     7021 ctcgcgggct ttgagaattt caatcaccgc atcccactga tcattagtga gatcggcacc
     7081 cgttgggttg tggcaacatg gatgcagcaa cacaatactg cgggcaggta atgttttcag
     7141 cgtcgccaac aggtcattaa agcgcacgct gttagtcact tcgtcatacc aggggtaagt
     7201 actcacttcg aatccagccc cggcgaatat tgctacgtgg ttttcccaag taggatcgct
     7261 gacccagacg cctgattccg ggaagtagcg tttcaggaaa tccgcgccca ctttcaatgc
     7321 ccctgagccg ccaagggttt gaatggttgc tacgcgctgt tgttgcagta ccggatggtc
     7381 cgcaccaaac agcagcggcg caatggcatg gcgatagctg ttaagccctt ccatcggtaa
     7441 ataaagcgaa gcgccatggg gctgcgcatt caggcgcgct tccgcctccg ccacggcttt
     7501 caattgtgga ataattccgt cttcgttgta gtacagaccg atacttaaat tcactttgtc
     7561 gctgcgaggg tcttctttaa aacgctccat aagcgtaaga atcgggtcgc cagcgtaggc
     7621 gtcaactttt tgaaacacgc gatggttctc caggtttacg ggcaggtggt taaaacacaa
     7681 taaaccggaa gaaggcgaag atcgagtgga tgttcaggag cgaacggcaa ttagcaacag
     7741 agtgagacta atgataaacg tacatccgcc agaggcacga cctttataag agcatgaaag
     7801 aacatcaact tattgaattt ttaggatttt attggccgga taaggcattc acgccgcatc
     7861 cggcacagac aatcaaatat tacagaacga ttaatccacg tatttcatcg cgacccttga
     7921 agtcaggcgc gtaataagtt cgtaagcgct tactttcgtc atttcagcga tacgttctac
     7981 gggcaaacct tcgccccata aaatgaccgg atccccggct ttgtcctgcg cctgtggacc
     8041 taagtctacg cagatcatat ccatcgccac gcgcccgaca atcggtactt cgcgaccgtt
     8101 caccagcact ggcgtaccgg acggcgcggc gcgcggataa ccatcgccat agcccatcgc
     8161 gactacgcca agacgggtat cacgttcgct tacccaggtt ccaccataac caacaggctc
     8221 tccggcttta tgctcacgca cggcaatcag gctggaggtt agtgacatca ctggctgaca
     8281 gccaaaatcg gcaccggtgg agcgatcttc cagcggcgag acgccataaa gaatgatgcc
     8341 cgggcgcacc cagtcaaaat gcgactgtgg ccacagcaga atgccacccg acgcggcaat
     8401 ggaacgttga ccaggtttgc cttcgcaaaa ggtattaaag atagcgagtt gtttctcggt
     8461 tgcgccacat tttggttcat ccgcgcgcgc aaaatggctg acgatattca ccggctgacg
     8521 aacgtttttg cactgggtca ggcgatgata aaacgcctca gcctgttccg gccttacgcc
     8581 cagacggtgc ataccggtat cgagtttcat ccagacggta accggctcgt ccaagctagc
     8641 ctcttccagc gcagccagct gttcttcgtt atgcacggcg gtatgaaaat gttgcgcaga
     8701 aatcgtcggc agatctctgg catcaaaaaa gccttcgagt aacagtacag gtttggtgat
     8761 tccccccgca cgcagtcgca gagcttcttc gagacgggct acgccaaagg cgtcagcatc
     8821 ggggagcgtt cgcgcggtct caagaagacc gtgaccataa gcgttcgctt tcaccaccgc
     8881 aaccatttta ctggcaggtg ccagttcacg aagacgttgc aggttgtgtc gcagagcgcg
     8941 gcggttaatc acaacagttg ccgcttgcat ttgtgttcct tgataattgt ttgctttaat
     9001 tacctaattc ataaaataat tattattcgt cgtcgtactg cggccccgca tagttgtcga
     9061 agcgcgacca ttgaccgtta aaggtcaggc gtaccgtccc gattgggccg ttacgttgtt
     9121 taccgataat aatttccgcg atgcctttta aatcactgtt ttcgtgatac acctcatcac
     9181 gatagataaa catgatcaag tccgcatcct gctcgataga gccagattca cgcaggtcgg
     9241 agttgaccgg acgtttgtcg gcacgttgtt ccagagaacg gttcaactgg gacagcgcca
     9301 ccaccggcac gttcagttct ttcgccagtg ctttcagcga gcgggagatt tctgcaattt
     9361 ccagcgtacg gttatcggaa agcgccggta cgcgcatcag ttgcaggtag tcgatcatga
     9421 taagcccgat gccgccgtgt tcacgggcaa tacggcgtgc gcgggaacgc acttccgttg
     9481 gcgtcaggcc ggaagagtca tcgatataga tattgcgttt ttcgagcaaa atacccatgg
     9541 tgccggaaat gcgcgcccag tcttcatcat cgagctgccc ggtacggatt ttagtctggt
     9601 caacgcgcga cagcgacgcc agagaacgca tcatgatctg ttctgatggc atctccagcg
     9661 agaagataag tactggttta tcctgcaaca ttgccgcgtt ttcgacgagg ttcatcgcaa
     9721 atgttgtttt acccatcgac ggacgcgcgg cgacgatgat caaatccgac ggctgcaagc
     9781 cagcggtttt tttgttgaga tcgtcataac cggtgtttac cccggtaacg ccatcgtgtg
     9841 gctgctgaaa caactgctca atacgcgcca cggttgcgtc gagcacatcg gcgatgttct
     9901 tcggcccttc gtctttgttt gcacgacttt cggcaatttt aaagacgcgg gattcagcaa
     9961 ggtccagcag atcttcgctg gtacgcccct gcggatcaaa accggcttca gcaatctcat
    10021 tcgcaaccga gatcatctca cggacaacgg cacgttcacg cacgatgtcc gcataagcac
    10081 tgatgttcgc cgcacttggc gtattttttg acagctctgc cagataagca aaaccaccga
    10141 cgctatcgag ttgcccctgg cgttccaacg attccgcaag ggtaatcaga tcgataggac
    10201 taccgctttc ctgcaaacgc gccatttcag taaagatatg acggtgtggg cgggtgtaaa
    10261 aatcgtctgc taccacacgc tcggctacat catcccagcg ttcgttatcc agcattaaac
    10321 cgcccaacac cgactgctcc gcttcgatcg agtgcggagg cactttcagc ccggcaactt
    10381 gtggatcgcg ttcgcggggt tcagcctgct gtttgttgaa gggtttattt cctgccatag
    10441 tgaatggagt taccgaaata aagaatgggt cgaaacttta ccatatgaag cagaccctga
    10501 cgatacgttc tggaggacac atggcaacac gaattgaatt tcacaagcac ggtggcccgg
    10561 aagtacttca agccgtagag ttcactcctg ccgatccggc ggagaatgaa atccaggtcg
    10621 aaaataaagc catcggcatc aattttatcg acacgtatat ccgcagcggc ctttacccgc
    10681 cgccatcgct acccagcgga ttaggtaccg aagcggcagg catcgtgagt aaagtcggca
    10741 gtggtgtaaa gcatattaag gctggcgatc atgtagtcta tgcgcagtcg gcgttaggcg
    10801 cttacagctc tgtgcataac attaatgcgg ataaagcggc gattctgcct gcggcaattt
    10861 cttttgagca agctgcggca tccttcctga aaggcttaac ggtttattat ctgctgcgca
    10921 aaacctatga aattaaaccc gatgaacagt tcctgttcca cgcagcggct ggcggcattg
    10981 gcttaattgc ctgccagtgg gcaaaagccc tgggcgcgaa actcatcggc accgtaggaa
    11041 ccgcgcaaaa agcgcagagc gcgctaaaag ccggcgcgtg gcaggttatt aactatcgtg
    11101 aagagaatct ggtcgagcga ttgaaagaga tcaccggcgg taagaaagtg cgcgtagtgt
    11161 acgattccgt gggcagagac acctgggaac ggtcgctgga ttgcctgcaa cgccgcggct
    11221 taatggtcag ttttggcaac tcatcaggtg cggttaccgg tgtgaactta ggcattctca
    11281 atcaaaaagg ctcgttgtat gtgacacgcc cttccctgca aggctatatc accacgcggg
    11341 aggaattaac cgaggccagt aatgaactgt tctctttgat tgccagcggt gtgattaagg
    11401 tcgatgtcgc cgagcagcag aaatatccgc tgaaggatgc gcagcgtgcg catgagattc
    11461 tggaaagccg ggcgacgcaa ggttccagcc tgctgattcc ataaaagaaa tagggcttcc
    11521 acctgggaag ccctttcttt ttatagttcg gctgtatgta gggtacagca cgatgaatct
    11581 gttagaggcg caatagtgac agatttgatt atcaattcct attttgttct aaggataaaa
    11641 ccttaggttg tgatcatccg cacaatccct tagtaacgcc agcggtcata acgctgatat
    11701 ttcggcattt ttggtgcttt aatcgcctta ataacccaca ccaccgcaat cgccagtagt
    11761 aaccacggca gcaacttaat catcaatgcc agcataccgc cgaggaacat aatggccgtc
    11821 gccacaacca gcgcggcgat aatgcccagc aacgaaacgc cggtgaccat cagcatgaca
    11881 aaaaagccaa tcacaaaaag tagttccagc atgatgctct cccaaatatg aaatctcttg
    11941 ctggcattac aagaatcatg ccaaaaataa tctattgatt taacagcaaa acgccccgcg
    12001 acggtgcgca gggcgtggtg aatttgacta ctttttggtg aaaagttaac gcttatccgc
    12061 caccagtttg agcgcgtgtt ccagcacgtt aatgtcagca cccgctttat gggcattttc
    12121 acttaaataa cgccgccact gccgcgcgcc aggaataccc tggaacaaac ccagcatatg
    12181 ccggataata tggccgagat acgtcccctg gctgagttca tgctcaatgt acggatacat
    12241 ggcacgcact actgccaccg gatcggcatc ggtatccgag gaaccaaaga tctcccggtc
    12301 taccgccgcc agaatacccg gattctgata cgcctcgcgc ccgaccatca cgccatccat
    12361 atgttgcaaa tgcgctttag cttcttccag cgacttgata ccaccgttaa tcgacattgt
    12421 cagatgcggg aagtcacgct tcagttgata cacacgcgga taatcgagcg gcgggatttc
    12481 acggttttct ttcgggctta accccgaaag ccaggcttta cgtgcgtgga tgatgaacat
    12541 ctcacactcg cctttgccgg aaacggtatt gatgaaatcg cagagaaatt catagctgtc
    12601 ctggtcgtcg atgccaatac gcgttttcac cgtcaccgga atcgacacca catcgcgcat
    12661 cgctttcacg cagtcggcaa ccagctgcgc attacccatc agacacgcac caaacatgcc
    12721 gttctgcacc cggtcagacg ggcagccgac attcaggttg atctcatcat atccgcgcgc
    12781 ttctgccagc tttgcacact gcgccagcgc cgccggatcg ctaccgccca gttgcaacgc
    12841 taccggatgt tcttcttcac tgtacgccag ataatcacct ttaccgtgaa ttatcgcccc
    12901 tgtggtcacc atttcggtat aaagcaacgt attgcgggaa agcagacgca agaaatagcg
    12961 gcaatgtctg tccgtccagt cgagcattgg tgcaacacta aaccgaccac tccagtgaac
    13021 gtcagttttt tcaggcattg cgctggtttg gttgattttt tgcatttcag aattaccgtg
    13081 cattttcaaa tgtagagatt attttatcga tatatcatgg ggttatgtta ttcagcttca
    13141 ctgttcagga ggctcaatag cggggtacta taccataaca acaggaagcg cctgtctcat
    13201 tgcaaaagaa aattgagatc ctctcaaggc atgaagctct cacgaagtga tggaaataat
    13261 cttattagcc gttaactttg ttaaggccaa tgataaacaa tccaggttcg acgataaata
    13321 aaaaatcaca cattaaactc tggtgatata tctccctgct aatagcattg atagagaaaa
    13381 aagaacccaa taaatattgg gtccttttat ataatgcctt ccatactatc gaagaacttc
    13441 acatattatt tctccgattt aaccccgaac aagtcataaa ttaatttaga agtgtctgta
    13501 agtattttaa tctcttcctt tgaggttggg tcaaacgact tcgcgaagct aattaattgt
    13561 ggtgcagcat ctcgcatttt gcttaaaata tcaggatcga gcgttccttc attcaccaga
    13621 tgtgacatct tatccagata gccatcaaaa ttcatttctc ccccatctgc cagacgcttc
    13681 atctctccca ggtacttctt catatcacgt tgatataatt tttcaaactg agctttcagg
    13741 tagctctcat tattttcatt aatatacact gtttctgata aatctgcgta ggcactagaa
    13801 tatgaaagtg aaattaacag cgaagcaaat aattttaagc tgttgtcatc atgttcctgg
    13861 caggcattaa caaacgttaa aaaaccagaa cctattgctt gaaaatgtat attcgctaaa
    13921 ggttcattat ccttagattc ctcataaaat agctgaaccg tggagggaag gccattctta
    13981 gtttctacat caaaagtaca ttgttcaatg ggcattgcat ctttattatc cccaggatta
    14041 catatactat taatcgcgaa atgaaaaata gcccggtcaa caactgatgc aataaagtca
    14101 cgatcgttta aatcctcatc agtgcaatcg tcagtatact tattgaccca cattttgaga
    14161 tcatttatgt gctcaattaa aaacacattt ttttacgtgc atgccataaa ttatgcgtga
    14221 ttcgttgaat aaacaaatct atttttgcaa taattatatc ccgatgacgc agcgctgcat
    14281 aatcaataga tttaccatat atattattta atttaccttt gattgaacct ctattatcca
    14341 atttatcttt ggttgcatct ttattttcga attgatttga attgagtgtt ttattaaagt
    14401 atccagttgc accgggtatt atcttcaaca tgattcatct accttttttg tcgtaccgtg
    14461 tcctccttat aattcttttg ccatctggtc actatcaaaa atcgctcata atttctgaac
    14521 tacaggtatg cgtttaaacc cagttaaata aatagcgagt taacttgaag caatttattt
    14581 aaaaatgaat tattaattat tatcgtgttg ataatggtac gataaggcaa cacaacacct
    14641 tacctacaaa acagaaaatc aacattacgg caacaataag ggttctcggt gtttttcctg
    14701 gaacatggta aagtaaggac attcttaacc cccactttga ggtgcccgat ggaaaagacc
    14761 acaacgcagg agttattagc gcaggctgaa aaaatctgcg cgcagcgtaa tgtgcgcctg
    14821 accccacagc gcctggaagt gttgcgcctg atgagtctgc aggatggcgc tatcagcgct
    14881 tatgatctgc ttgatttact gcgcgaagct gaaccgcaag ccaagccgcc aacggtttat
    14941 cgcgcgctgg attttctgct tgagcaaggt tttgtgcata aggtggaatc caccaacagt
    15001 tatgtgctct gtcatctgtt cgaccagccc acgcatacgt cagccatgtt tatttgcgat
    15061 cgctgcggcg cggtgaaaga agagtgtgca gaaggcgtgg aagacattat gcatacgctg
    15121 gcggcaaaaa tggggtttgc cctgcggcat aatgtgattg aagcacatgg gctctgtgcg
    15181 gcatgcgtgg aagtggaagc gtgtcgtcat cctgaacagt gccagcatga tcactctgtg
    15241 caggtgaaaa agaaaccgcg ttaagagggc gtacatcctt gtacacgtcg ggcaggaggg
    15301 attaattacc agcgatattc attgcgggtt tcccaatcca cgacctcttt ttctgcctga
    15361 tctttctgat aaccataacg ttcctggatt ttaccgacca gttgatcacg tttaccttca
    15421 atgatcgtca tatcatcatc ggtcagtttg ccccattgct ctttcacttt acctttaaac
    15481 tgtttccagt taccgccggc ttcatcttta ttcataatca agacctcatc gttaggttgt
    15541 gaatgagagt acgttcactt ttcttctgaa cgtgagatta agtgtagtca gcaatttggc
    15601 ttaggataat ttattcagaa tttttaaccg tcacgttgcg gcaaaccagg taccgttgcg
    15661 ccagtgacgc cgccagatag ccgccagaga aagcccgcgc aacgccagaa agacggttaa
    15721 tgccagccac aaaccatgat tacccagcca cggcagcgta aggagcgtca gcgcaaaacc
    15781 tgcggcggcc accgccatac tgttacgcat ttcggcggca cgcgttgcgc ctataaacat
    15841 gccgtccagc agataacacc agacgccaac caacggcaaa atcacctgcc agataagata
    15901 gcggtcagcc agctgctgaa tctgggttaa cgacgtcagc aacgcaatga tgtgttcccc
    15961 tgccagcaaa taaaccaccg aaaatagtaa cgctacaatc cccgactggc ggcacgctgc
    16021 ccgccataca tccagcaact ggctaccgtc gcgcgcaccg tatgcctgac cggagtgcgc
    16081 ttcaaccgcg taggcaaaac catcaagcgc ataggcggta aaggtgagta gcgtcatcag
    16141 aaccgcgtta acagcgataa tgtcactccc cagtcgcgca ccaagtacgg tgatcgcgcc
    16201 gaaacagagt tgcaacaaca gcgaacgcag catgatatcg cggttaagcg ccagcaagcg
    16261 acggaagttt cctcgccagg cagttttcag catttcgccg gaaattccgc gtagtttgag
    16321 gattttacgc accattagca gaccaatcag caatgttgca tattccgcaa taaccgtcgc
    16381 cagcgccgcg ccctgcacgt tcatatgcag ccccatcacc agccagacat ccagcacaat
    16441 gttgaggata ttgccgacca ctaacaaaat tactggcgca cgggcatatt gcacgccgag
    16501 taaccaaccg agtaatacca gattcgccag cgacgccgga gcgcttaacc agcggatttc
    16561 aagaaagcgc cgcgcctgtt ccaggaccgc ttcactaccg ccaacaatat gcagcgccag
    16621 atcgataatc ggcgtacgca acagcgcaat taacgccccg gcccccaacg ccaacagcaa
    16681 cggttgcacc agcgcacggg ctaatgcctg aggatttttg gcaccataag cctgcgcagt
    16741 cagcccggtg gtgctcatgc gtaaaaacag caacagcata aagagaaagc tggtcgccgt
    16801 tgcaccaacc gccacgccgc ccaaataaac cggactatca agatgaccaa ttaccgccgt
    16861 atcgaccagt cccagcaacg gaacggtgat attggagaaa atcatgggta aggcgagatg
    16921 ccagagtgct ttatcagatg aagtgaggaa tgccatgcag acaagcctga tgaagagaga
    16981 tgaaaaacaa accgcgacgc caagcggcat cgcggtctca gagatatgtt acagccagtc
    17041 gccgttgcga ataaccccaa ccgccagccc ttcaatggtg aagctctgct gacgaaggtc
    17101 aacgacaatt ggtttaaact cgctattttc tggcaacagt tcgactttat tgccctgttt
    17161 tttcaggcgc ttaacggtaa cttcgtcatc aatacgtgcg acaacgacct gaccgttacg
    17221 tacatcctga gttttatgca ctgccagcaa gtcgccatcc ataatgccga tatctttcat
    17281 cgacatcccg ctgacgcgca gcaggaaatc agcattcggc ttgaacaagg aaggatcgac
    17341 ctgataatga ccttcaatat gctgttgcgc cagaagcggt tcaccggcag ccacacgacc
    17401 taccagcggc aacccttctt cctcttcctg caacagacga atcccgcgtg atgcgccgga
    17461 aacaatttca ataacgcctt tgcgtgccag cgccttcaga tgttcttcag ccgcgtttgg
    17521 ggaacggaac cccaaacgct gcgcgatttc cgcacgcgtc ggcggcatac ctgtctggct
    17581 gatgtgatca cggatgagat caaacacctc ttgttgcctg gccgttaacg ctttcattcc
    17641 gccccctggg tgtatataca gttatactgt gagtatatac agcaaaaggc gattttggaa
    17701 ccataaactg cacaataaac cagagattta tcgaaatctg gaagggttat ccaaaatgcg
    17761 accataacag gatgcaccag gtaatcacgg cgacgataat ggcaatcagc accgcggcgg
    17821 atcccatatc ttttgcgcgt ccggaaagct catggtattc agagccaatt cggtcaacca
    17881 ctgcttcgat ggcgctattg aggatttcca caatcatcac cagcatcacg gagctgataa
    17941 gcagcacgcg ggtaattgcg tccacatcca gccagcaggc gatgaccacc gccaacaata
    18001 ccgctacgcc ttcctgacgg aatgccgctt cgttgatcca tgcagcgcgt aaacctttcc
    18061 aggaatagcc agcagctttg ataattcggg tgaatccagt tgtattattg gccattgaaa
    18121 gaaccttttt acattatgag cgtcaatatc agtgtaccgt taaatccgat gattcgggtt
    18181 tgcgtaacgc cgcgcgaaac atgagcggat accacagaat ttcccatgac tttctgctat
    18241 ccttgccgcg catttgcatt attaaccaga ggctttacat cgtttatgtc cggctggcca
    18301 cgaatttact acaaattact gaatttacca ttaagcatcc tggtaaaaag caagtctatt
    18361 ccggcagatc ctgccccgga actggggctg gatacctctc gtccaattat gtacgtttta
    18421 ccgtacaact cgaaagcaga tttgctgacg ttgcgcgccc agtgtctggc acatgatttg
    18481 cctgacccgt tagagccgct ggaaatcgac ggcacgctac tgccgcgcta tgtgttcatt
    18541 cacggcgggc cgcgtgtatt cacctattac acgccgaaag aagagtctat taagcttttc
    18601 cacgactatc tcgatttgca ccgtagcaac ccaaatctgg atgtgcagat ggtgccagta
    18661 tcggtgatgt ttggtcgcgc gccggggcgt gaaaaaggcg aagtgaaccc gccgctgcgt
    18721 atgcttaacg gcgtacagaa atttttcgct gtactgtggc tcggtcgcga cagttttgtg
    18781 cgtttctcgc cgtcagtgtc gctgcgccgt atggcggatg aacacggcac ggataaaact
    18841 atcgctcaga aactggcgcg cgtggcgcgt atgcactttg cccgtcaacg tctggctgcc
    18901 gtaggcccac gtctgcctgc tcgtcaggat ctgtttaata agctgctcgc ctcccgcgcc
    18961 attgccaaag cggtagaaga tgaagcgcgc agcaaaaaaa tctcccatga aaaagcgcag
    19021 cagaacgcga ttgcgctgat ggaagagatt gcggcgaatt tctcttacga gatgatccgc
    19081 ctgactgacc gcattctggg cttcacctgg aaccgatttt atcagggcat caacgtccat
    19141 aacgccgagc gcgttcgcca gctggcccac gacggtcatg agctggtgta tgtgccttgc
    19201 caccgcagtc acatggacta cctgctactt tcttacgtgc tgtaccacca gggactggtg
    19261 ccgccgcata tcgccgccgg gatcaacctg aacttctggc cggccgggcc gattttccgc
    19321 cgtctggggg cgttctttat tcgccgtact tttaaaggca ataaacttta ttccaccgtt
    19381 ttccgtgagt atctcggcga actgttcagc cgtggttatt ccgtcgagta cttcgtggaa
    19441 ggcggtcgtt cccgcacggg gcgtttgctg gatccgaaaa ccggtacgct gtcgatgacc
    19501 attcaggcga tgctgcgtgg cggcacgcgt ccgattacgc tgattccgat ctatatcggt
    19561 tatgagcacg tcatggaagt gggtacttac gccaaagaac tgcgcggcgc gacgaaagag
    19621 aaagagagcc tgccgcagat gctgcgcggt ttaagcaagc tgcgtaatct cggtcagggt
    19681 tacgtcaact tcggtgaacc aatgccgttg atgacctacc ttaaccagca cgtaccagac
    19741 tggcgtgaat ctatcgatcc catcgaagcg gtgcgtccgg cctggttaac gccgacggtc
    19801 aataatattg ctgccgatct gatggtacgc attaacaacg caggcgcggc aaacgccatg
    19861 aacctgtgct gtactgcgct actggcatca cgtcagcgct cactcacccg cgagcagtta
    19921 accgagcaac tcaactgcta cctggatctg atgcgcaacg taccttactc cacggactct
    19981 accgttcctt cagccagcgc cagcgagctt atcgatcacg cgctgcaaat gaacaagttt
    20041 gaagtcgaga aagacactat cggcgacatc atcattctgc cacgcgagca agcggtgctg
    20101 atgacctact atcgcaacaa cattgcgcat atgttggtgc tgccttcgct gatggcggca
    20161 attgtcaccc agcatcgcca catctcccgc gacgtattga tggagcacgt caatgtgctt
    20221 tacccaatgc tgaaagcgga gctgttcctg cgctgggatc gcgacgagtt gccggacgtt
    20281 attgatgcgc tggcaaatga gatgcaacgt caggggctga ttaccctgca agatgatgag
    20341 ttgcatatca acccggcgca ttctcgcacc ctacagctgc tggccgcagg cgcgcgcgaa
    20401 acgctgcaac gttatgccat caccttctgg ttgttgagtg ccaacccgtc gatcaaccgc
    20461 ggtacgctgg agaaagagag ccgcaccgtt gcgcaacgtc tctccgtgct gcacggcatc
    20521 aacgcgccgg agttcttcga caaggcggtg ttcagttctc tggtgctgac gctgcgtgat
    20581 gaagggtata tcagcgatag cggcgatgcc gaaccagcag aaacgatgaa ggtttatcag
    20641 ttgctggcgg agttgattac atcagacgtg cgtttgacga ttgagagtgc gacgcagggc
    20701 gaagggtaat cagagagaat tgccggatgc ggcgaaaacg ccttatccgg cctaccatga
    20761 cctgcaaatt caataaattg cgattcaccg ggtaggcctg agaagcgcaa cgcatcaggc
    20821 aacccagaag aaagccggat gatcatccgg cttttttaca tcatcagaaa tgccagtaac
    20881 tcattgccag tcctaaaaac agtaccagac caacatagtt attattcata aatgctttaa
    20941 agcaggcttc acgctcgcgg ttggcaatca gtttttgttg ataaacaaac agcgcgccag
    21001 ccaccacaat tgaccagtaa tatccccagc ctaagccatt taactcaccg atgatcgcca
    21061 tcagtgccag tacgccaatc tgcaaaatac caataatcaa tttatcgtat tggccgaaca
    21121 ggattgccgt ggatttaatg ccaatcttca catcatcatc gcggtcaacc atcgcatact
    21181 gcgtgtcgta agccaccgcc cagagaatat tggcgaggaa cattaaccag caactcaatg
    21241 gcaccgactc actcacagcg gcaaaagcca ttggaatcga ccagccaaac gccgcgccca
    21301 gcaccacttg cgggagatgg gtataccgct tcataaacgg gtacacccac gccaatgcca
    21361 gagcggcaat cgacaacaga atggtcatcg tattcagcgt cagaaccagt aaaaacgaaa
    21421 tcagtaccag cactacaaac agcgcgcgcg cctctttctc tgttaccgcg ccgctgggaa
    21481 gtggtcggtt cgccgtgcgc ttaacatgac catcaaactt gcgatcagca taatcattca
    21541 ccacgcaacc agcagcgcgc atcagccaga cacccgcgac aaacaccgcc atgatccaga
    21601 gctgaggaac gcccggtgtc gccacccaca acgcccataa tgttggccag agcagcagta
    21661 acgcgccaat tggcttatcc gtacgcatta agcgatgaaa cgccagcagc ttattctgcg
    21721 tcagactcca ctccatattt ttttcctctt agtacaacgg tgacgccggt aaaaacagtt
    21781 ctgttagcaa cagcggttta ccgcttaatc gcaggcggga acgtcgcccc cacagtccgg
    21841 catcacggcc tatctcgata aaatcccggg ttagtgtcga tgatgtgaac agatagcgtc
    21901 ctaacggcgt tttacccaat ttttgtaacg ctagttccgg cccgcttaat gttgatacag
    21961 gaacgacggt acgaccggca agccacggtt caccatcggc acataacaaa atttcacgta
    22021 accagtaacg agactctttc ggcagcagcg gcagttcttc ggggatttca ttctgctcga
    22081 caaacccctc gcggatcatc gtcacactca ccgtttttcc ctgctgttca aaacgttttg
    22141 tcatggaatc ctccagcaac agccagtcga gcagttgcgg atccagggca gggatctctt
    22201 taaaatagcg cagcgcacgc agttgcgtta acgcggggtg tgacatgccg aactctccgt
    22261 tacataacgt aaaggcattg tatcgcagaa aagtgtttac gggagatgta aagcaaatta
    22321 agtgatctaa tgcgcaacaa aaacgcaaca atcgcatgaa gtggtaaaac gctgttgaac
    22381 cttacgcttt gccgatgccc gtgctggcga gggcaaccat aggggcatca ccgcccatga
    22441 tgctaggaac ggtgatgttt tcctttactt aaataccttt attctttata cgagtagctc
    22501 gccaactcgc agtaaaaatt gggaaaagcg catatttacc atgagatttg gagctgaaaa
    22561 gtgtttcctg agtaagatta ataggcaaca tcttatgtct ggatttatag atacacgtgg
    22621 aattccaatt ggtatctttt gtaggattca acatatcatg taagcgctgg gagcttacca
    22681 ccataacagg cccatcatcg acagaggaga acaggataaa tatattatcg tttcgtgtta
    22741 atttattagt gttaaaggtt cttaactttt cttcagtttc tataaatata ggtgaaacat
    22801 catcactacg aatccactct ttataaagat accaggcatt tgctttgatc atatcatccg
    22861 ttgatcgtaa ggcctgatcg ataagttgga taaaaaaatt gttattttta tcaaccagcc
    22921 ctttattcat attaaatctg ttaataaaat ggatcatctg ttgggtatcg aaattcacct
    22981 tactgaatcg atgttgacgc ataaaattat cgaacttgtc atcttcccca gaatgtatat
    23041 aactacgtat aattggatct tttatataag gacaatggct aaatgttttc ttcatattct
    23101 caataaagtt tttattaaca atattgagaa tttttaattg catcaccagt tttactgcca
    23161 gaacggattt caatatcatt gcattatctg gcaatgtatc aatcattttt ataatgttat
    23221 tgatataacc atcgagatct tcattcccaa atatatctga ataatcaata gttaattcca
    23281 attgttctgt aatgcaatct taaaaattcc ctgtcgacat tcgcacatga tcaaacataa
    23341 cagctgtcaa attcgattta ataaatgata tttctttggc agacagaaac tctgctctgg
    23401 tgaagtgcga agcgtaaaga taacaattat cgaagtttga atttctaatc tgacattcgc
    23461 gaaaattact atgaccaagt tgactctgcg agaagtcaac tgtatcaaga atattttggc
    23521 taaaatcaac acattcgcca ctcgttttga gaaaaatcac attgtgtaaa cgtcgacatt
    23581 taaatatgac attatcaact gttgccttag taaaattgca attctcaatt tctttacatt
    23641 cgaaagttac atgttgtaga tttgatgata aaaaagacac cttagaaata acgtcacctt
    23701 taaaatgaca atttgttaaa tcaaaatcag acaggtcgat atacccctgc gcatctttat
    23761 ccatcagaaa attacaagcg cattctattt tttcatttct gtagaatttt acacttatag
    23821 tatcattact aattgagact tcacgtaaca tatttattgc atgatttttt tcgttccgtt
    23881 gttttatatt taaaaatata tcactgatat aaataaagta atctctgttt aaacctgtca
    23941 ccccattgaa attacgtagt aaatcaagat ctactatatt atttccttgc accacatcat
    24001 gtttcatcaa agagttattg ccgccagaat ttgtgatatg acctatcata aaacctctgt
    24061 aatacagaaa taaaataacc tggaaaagta gcctgagcgt ccagacacta ccggcgaaag
    24121 gaaaagaatc tctgataatg cattgaaata atggacattc ttagtgaaga agaagtgaag
    24181 tactataaaa aatcgctcat atcccaggaa ggaagcataa aaaaaggtgc gccaggagac
    24241 gcaccagttg ttgcaaaatc agcactgtgg ggcgtaatta ccccttgcct tttacactgc
    24301 tgataaaggt ggaacgggca gatgtcgatc ccaggcgttc agcttcatca agcagtttta
    24361 acgccttatc aacatcacct ttcgcgacag cgtttttgat cgcggtatta aaataacttt
    24421 ccgtgtcgtt gagcatcggc tcgcttttct tcaccggcac cggagcaggt gcagccacag
    24481 ctggtgccgc cgtgttgcct accgtaaccg gagctggagc ggaagaacca aatagcggtc
    24541 ctaccaacac gctggagctg gagttcgttt tgactttcag tttcagtaag ccatcgctgg
    24601 tatgacgagc aaccggatcg ggaatatccg ggatcgagtt accgacgcct ttggcatagg
    24661 ctttagccgg gtcgagcagt tgggtcgtct gctggagatc tttttccgtg gtaaagacca
    24721 gaacataaag tttttgctgc cccaacgccg gtgtcaggcg cataacgcct tccagccgat
    24781 ctgcactcat cacgcctagt tcctggtagg tgaaataact gctggggaag aaggctgatg
    24841 gggtcatgtt ctgatcaaga atcagcacgt tcggcgcaaa aacgctggtt tgtttgttca
    24901 cttcgctggt cagcgtcagg gtcagttcgc caatgtttgc cgggacgctg tacgcagcaa
    24961 ccggaccact gatgccggga acgttcagtt gttggccgcc ggtcgccagt tgggtggtct
    25021 gggttttaga ttgatcgacc ggtgtccagg tgagttgttg cagcgcggca gatggaatgg
    25081 ctggcgcgtc gctggtgttt tgcggtacgt agttaacatc ggcaaggcta attccaggcg
    25141 cgcttgccag taaccctgct gataaacaga gggcgatgag acttttattc attttcattg
    25201 ttttcacctc aaaatctgga gctcagcggt agccaggcaa tagcgcgcta aacccgataa
    25261 tcagaggggc tttcgcccct ttcagataat gacaacctgt ttttatgccg gatgcggcgt
    25321 aaacgcctta tccggcccag gttttgctat taccaccaga tttccatctg ggcaccgaag
    25381 gtccactcgt cgctgtcgcc acgaccgaag ctgccgccgt tgaaatcagc aggaacggct
    25441 ttgccgtagt tcgggttaac cttgctatcg ccgttgtagt cgtaacccca tttctcatcc
    25501 cacttggcgt aggttgcgaa gacacgaata gccgggcgtg accagatgct gtcgccagcc
    25561 tgccattgtt gtgcgagggt aattttgtac tgattgttct tgtcgccggt gcgctgggat
    25621 tcgacgttgt cgtagccgat ttccatcacg gtgctcatga ttggcgtcca cttgtacatc
    25681 gggcgaatac caacggtcca ccacttggtg ccgttgtcgt tatcccagtt gatatcctgg
    25741 tacataccca cgtacatcat gtcccagttg tcgcccatgg agatcgcacc gtggtcgagg
    25801 atacgcagca tgtgaccgtt gttgttgata ttgtaggcaa atttttcgtt atcaaacgcg
    25861 acgccagaac cctgcgacag acctttaccc tgcgaggtca tcgagtcagt agcgtactga
    25921 acaacaaact tgttaaagcc cttcaggaca ctctgagtat gttcagcagt gaataaccag
    25981 ccgtctttcg atgcgccatc aaccagacga tagttatcac gcaggttggc acgaccgtag
    26041 tcgacaccca gttctaatgt gccgcccggg ttgatttcca tctgcgctaa acgcacatcg
    26101 aaaacgtcgt tcgcggtttc gttggtatag tcataaatat tgttgctggc aaaagaggaa
    26161 gaaccaccag cttcagagga gcgggttgct gccagagaga gtttaccgaa gccaacatcg
    26221 atgttttcca gaccggcacc aggaccagaa atatcccagt agtagaagtc gatcatatga
    26281 acgtcatgac gttggtagaa gcgcttacct gcccagatgg tggaacctgg cagccattcg
    26341 atcaggtttt ttccctgcac gtttgcttca cggaaggccg gatcggtagc ttcccagtca
    26401 ttctgttgcg cgacggaata ggccacgtta gtgtcgaaat agaagctctt atcgccctct
    26461 ttccacactt cctgacccaa ttttaattca gcataagttt cacattcgtt gccaagacgg
    26521 tatttacttt gagcaccggt agtctggaaa cactgttgtt caccgccgct acctgtccag
    26581 ccaataccgg aacgtgcata gccgtggaaa tcaacagcca ttgcctgagc agacattacg
    26641 cccgctgcga cggcaaccgc cagaggaagt ttgcgcagag taatcatcat tctatctcct
    26701 gagtcattgc ttttcttttt tcacatcacc tgtgacaggc tttgtgtgtt ttgtggggtg
    26761 cttaaacgcc cggctcctta tgcagtcgac gacatgcagt gccatcctca cggaacagat
    26821 ggcaacgctc tggcggcagg ccgatagcga atgtggcacc ttcttctacc aacaccacgt
    26881 cgttctggcg gtacaccagg ttttgacgaa tggaagggat ctggatatag atttgggttt
    26941 cgttgccgag ttgctcgacg acctgaactt caccctcaag gatgacgtca gcgatatcac
    27001 tcggcagtag atgttccggg cgaataccca gcgacatatt ggctccaacc tggacatcac
    27061 ggctttcaac aggcagccag acttgttgac gatttggcat cggcagctcc acctgcactt
    27121 gatcgattgc ggtggcggtc acttttaccg gcaggaagtt catctttggc gaaccgataa
    27181 atccggcgac aaaacggtct gccggatagt ggtacagttc cagcggtttc ccaacctgtg
    27241 ccacgcgacc ggcgtccagc accacgattt tgtcggccag cgtcatcgct tcgacctgat
    27301 cgtgggtgac gtaaatcatt gtgcggccca ggcgtttatg cagacgggag atttcgatac
    27361 gcatttgcac acgcagtgca gcatcgaggt tggagagcgg ttcatcgagc aaaaatacgc
    27421 ttggctcggc caccagcgta cggccaatcg ccacgcgctg acgctgacca ccggagagcg
    27481 ctttcggttt gcgatccagc aaatgcgcca gttgcagcac ttccgccacc tggttgacgc
    27541 gttggttaat cacctctttt tttgcgccag ccagtttcag gccaaatgac atgttttctg
    27601 ctactgacag gtggggatag agcgcgtaag actgaaacac cataccaacg ccgcgttctg
    27661 ctggcggagt gtcattcatc cgtttctcac cgatgaacag gtcgccgctg gtgatcgtct
    27721 caagcccggc aatcatgcgc agtaaagtcg atttaccgca gccagacggt ccgacaaaca
    27781 ccacgaattc accttcatgg atatcgagat tgatatcttt cgataccacg acctcgcccc
    27841 aggctttcgt tacattttgc agctgtacgc tcgccatgcc cttctccctt tgtaacaacc
    27901 tgtcatcgac agcaacattc atgatgggct gactatgcgt catcaggaga tggcttaaat
    27961 cctccacccc ctggcttttt tatgggggag gaggcgggag gatgagaacg cggcttctgt
    28021 gaactaaacc gaggtcatgt aaggaatttc gtgatgttgc ttgcaaaaat cgtggcgatt
    28081 ttatgtgcgc atccccacat taccgccaat tctgtaacag agatcacaca aagcaacggt
    28141 ggggcgtagg ggcaaggagg atggaaagag gttgccgtat aaagaaacta gagtccgttt
    28201 aggtgttttc acgagcactt caccaacaag gaccatagat tatgaaaata aaaacaggtg
    28261 cacgcatcct cgcattatcc gcattaacga cgatgatgtt ttccgcctcg gctctcgcca
    28321 aaatcgaaga aggtaaactg gtaatctgga ttaacggcga taaaggctat aacggtctcg
    28381 ctgaagtcgg taagaaattc gagaaagata ccggaattaa agtcaccgtt gagcatccgg
    28441 ataaactgga agagaaattc cctcaggttg cggcaactgg cgatggccct gacattatct
    28501 tctgggcgca cgaccgcttt ggtggctacg ctcaatctgg cctgttggct gaaatcaccc
    28561 cggacaaagc gttccaggac aagctgtatc cgtttacatg ggatgccgta cgttacaacg
    28621 gcaagctgat tgcttaccca atcgctgttg aagcgttatc gctgatttat aacaaagacc
    28681 tgctgccgaa cccgccaaaa acctgggaag agatcccggc gctggataaa gaactgaaag
    28741 cgaaaggtaa gagcgcgctg atgttcaacc tgcaagaacc gtacttcacc tggccgctga
    28801 ttgctgctga cgggggttat gcgttcaagt atgaaaacgg caagtacgac attaaagacg
    28861 tgggcgtgga taacgctggc gcgaaagcgg gtctgacctt cctggttgac ctgattaaaa
    28921 acaaacacat gaatgcagac accgattact ccatcgcaga agctgccttt aataaaggcg
    28981 aaacagcgat gaccatcaac ggcccgtggg catggtccaa catcgacacc agcaaagtga
    29041 attatggtgt aacggtactg ccgaccttca agggtcaacc atctaaaccg ttcgttggcg
    29101 tgctgagcac cggtattaac gccgccagtc cgaacaaaga gctggcgaaa gagttcctcg
    29161 aaaactatct gctgactgat gaaggtctgg aagcggttaa taaagacaaa ccgctgggtg
    29221 ccgtagcgct gaagtcttac gaggaagagt tggcgaaaga tccacgtatt gccgccacca
    29281 tggaaaacgc ccagaaaggt gaaatcatgc cgaacatccc gcagatgtcc gctttctggt
    29341 atgccgtacg tactgcggtg atcaacgccg ccagcggtcg tcagactgtc gatgaagccc
    29401 tgaaagacgc gcagactcgt atcaccaagt aatgctgtga aatgccggat gcggcgtgaa
    29461 cgccttgtcc ggcctacaaa actgaaacgc atgtaggcct gataagacgc atcagtgtcg
    29521 catcaggcag ttgttgtcgg ataaggcgtg aaagccttat ccgtcctgga atgaggaaga
    29581 accccatgga tgtcattaaa aagaaacatt ggtggcaaag cgacgcgctg aaatggtcag
    29641 tgctaggtct gctcggcctg ctggtgggtt accttgttgt tttaatgtac gcacaagggg
    29701 aatacctgtt cgccattacc acgctgatat tgagttcagc ggggctgtat attttcgcca
    29761 atcgtaaagc ctacgcctgg cgctatgttt acccgggaat ggctggaatg ggattattcg
    29821 tcctcttccc tctggtctgc accatcgcca ttgccttcac caactacagc agcactaacc
    29881 agctgacttt tgaacgtgcg caggaagtgt tgttagatcg ctcctggcaa gcaggcaaaa
    29941 cctataactt tggtctttac ccggcgggcg atgagtggca actggcgctc agcgacggcg
    30001 aaaccggcaa aaattacctc tccgacgcgt ttaaatttgg cggcgagcaa aaactgcaac
    30061 tgaaagaaac gaccgcccag cccgaaggcg aacgcgcgaa tctgcgcgtg attacccaga
    30121 atcgtcaggc gctgagtgac attaccgcca ttctgccgga tggcaacaaa gtgatgatga
    30181 gctccctgcg ccagttttct ggcacgcagc cgctctacac actcgacggt gacggcacgt
    30241 tgacgaataa tcagagtggc gtgaaatatc gtccgaataa ccaaattggc ttttaccagt
    30301 ccattaccgc cgacggcaac tggggggatg aaaagctaag ccccggttac accgtgacca
    30361 ccggctggaa aaactttacc cgcgtcttta ccgacgaagg cattcagaaa ccgttcctcg
    30421 ccattttcgt ctggaccgtg gtgttctcgc tgatcactgt ctttttaacg gtggcggtcg
    30481 gcatggttct ggcatgtctg gtgcagtggg aagcgttgcg cggcaaagcg gtctatcgcg
    30541 tcctgctgat tctgccctac gcggtgccat cgttcatttc aatcttgatt ttcaaaggat
    30601 tgtttaacca gagcttcggt gaaatcaaca tgatgttgag cgcgctgttt ggcgtgaagc
    30661 ccgcctggtt cagcgatccg accaccgctc gcacgatgct gattatcgtc aatacctggc
    30721 tgggttatcc gtacatgatg atcctctgca tgggcttgct gaaagcgatt ccggacgatt
    30781 tgtatgaagc ctcagcaatg gatggcgcag gtccgttcca gaacttcttt aagattacgc
    30841 tgccgctgct gattaaaccg ctgacgccgc tgatgatcgc cagcttcgcc tttaacttta
    30901 acaacttcgt gctgattcag ctgttaacca acggcggccc ggatcgtctt ggcacgacca
    30961 cgccagccgg ttataccgac ctgcttgtta actacaccta ccgtatcgct tttgaaggcg
    31021 gcgggggtca ggacttcggt ctggcggcag caattgccac gctgatcttc ctgctggtgg
    31081 gtgcgctggc gatagtgaac ctgaaagcca cgcgaatgaa gtttgattaa gggagataac
    31141 aaaaatggca atggtccaac cgaaatcgca aaaagcacgt ttgtttatta ctcacctgct
    31201 actgctactt tttatcgcgg cgattatgtt cccgctgctg atggtcgtcg ctatctcgct
    31261 gcgtcaggga aacttcgcga ccggtagcct gatcccggag caaatctcct gggatcactg
    31321 gaaactggcg ttaggtttta gcgttgaaca ggctgatggt cgcattacgc caccgccatt
    31381 cccggtactg ctgtggctgt ggaactcggt aaaggtcgct gggatttccg cgattggcat
    31441 tgtggcgctc tccaccacct gcgcctacgc tttcgcccgt atgcgctttc caggcaaagc
    31501 gacgctgctg aaaggaatgc tgattttcca gatgttcccg gcagtacttt cactggtcgc
    31561 gttgtatgcg ttgtttgatc gtctgggtga gtacattcca ttcattggcc tgaatactca
    31621 cggcggcgta attttcgcgt atctgggtgg gattgcgctg catgtctgga ccatcaaagg
    31681 ctatttcgaa accatcgaca gttcgctgga agaagcggct gcgctggatg gtgcgacacc
    31741 gtggcaggcc ttccgccttg tcctgttgcc gctgtcagta ccgattctgg cggtggtatt
    31801 catcctgtcg tttatcgctg ccattactga agttccggtc gcgtcgctgt tactgcgtga
    31861 cgtaaacagc tacaccctgg ccgtggggat gcagcaatac ctcaacccgc aaaactacct
    31921 gtggggtgac tttgccgccg ctgccgtgat gtctgcatta ccgatcacca tcgtcttctt
    31981 gctggctcaa cgctggctgg tcaacggcct gacggcaggt ggtgtgaaag gttaaagatg
    32041 ttgttctgcc aatgttatgc cgctgcaccc tcaacttacg ttatcccaac ttgtgactat
    32101 tattcggcgc tccacggagc gccttttttt ctttcgtctg caatctgaat cgttcgccgg
    32161 ttaatattcc catcatagaa cttattattt ttacgttatt tgttttccca cttacgataa
    32221 ttctctttcg tgctctgagt cacggcaata gtattgtttc atcaattttt gataattatc
    32281 acaattaaga tcacagaaaa gacattacgt aaacgcattg taaaaaatga taattgcctt
    32341 aactgcctga caattccaac atcaatgcac tgataaaaga tcagaatggt ctaaggcagg
    32401 tctgaatgaa tacccagtat aattccagtt atatattttc gattacctta gtcgctacat
    32461 taggtggttt attatttggc tacgacaccg ccgttatttc cggtactgtt gagtcactca
    32521 ataccgtctt tgttgctcca caaaacttaa gtgaatccgc tgccaactcc ctgttaggat
    32581 tttgcgtagc cagcgctctg attggttgca tcatcggcgg tgccctcggt ggttattgca
    32641 gtaaccgctt cggccgtcgt gattcactta agatttctgc tgtcctgttt tttatttctg
    32701 gtgtaggttc tgcctggcca gaacttggtt ttacctctat aaacccggac aacacagtac
    32761 ctatttatct ggcaggttat gtcccggaat ttgttattta tcgtattatt ggcggtattg
    32821 gcgttggttt agcctcaatg ctttcaccaa tgtatattgc ggaactggct ccagctcata
    32881 ttcgcggaaa actggtttca tttaaccagt ttgcgattat tttcgggcaa cttttagttt
    32941 actgcgtaaa ctattttatt gcccgttccg gtgatgccag ctggctgaat actgacggca
    33001 ggcgttatat gtttgcctcg gaatgtatcc ctgcactgct gttcttaatg ctgctgtata
    33061 ccgtgccaga aagtcctcgc tggctgatgt cacgcggcaa gcaagaacag acggaaagta
    33121 tcctgcgcaa aattatgggc aacacgcttg caactcaggc agtacaggaa attaaacact
    33181 ccctggatca tggccgcaaa accggtggtc gtctgctgat gtttggcgtg ggcgtgattg
    33241 taatcggcgt tatgctctcc atcttccagc aatttgtcgg catcaatgtg gtgctgtact
    33301 acgcgccgga agtgttcaaa acgctggggg ccagcacgga tatcgcgctg ttgcagacca
    33361 ttattgtcgg agttatcaac ctcaccttta ccgtactggc aattatgact gtggataaat
    33421 ttggtcgtaa gccactgcaa attatcggcg cactcggaat ggcaatcggt atgtttagcc
    33481 tcggtaccgc gttttacact caggcatcgg gtattgtggc gctactgtcg atgctgttct
    33541 atgttgccgc ctttgccatg tcctggggtc cggtatgctg ggtactgctg tcggaaatct
    33601 tcccgaatgc tattcgtggt aaagcgctgg caatcgcggt ggcggcccag tggctggcga
    33661 actacttcgt ctcctggacc ttcccgatga tggacaaaaa ctcctggctg gtggcccatt
    33721 tccacaacgg tttctcctac tggatttacg gttgtatggg cgttctggca gcactgttta
    33781 tgtggaaatt tgtcccggaa accaaaggta aaacccttga ggagctggaa gcgctctggg
    33841 aaccggaaac gaagaaaaca caacaaactg ctacgctgta atcttcctgt ccagcacgcc
    33901 gcgccatttc ggcgtgctgt ctttttactc ccgcttcagc cgtttcgaat tacacagcca
    33961 cagggtgatc accagtaaca ggatcgcagc cgagtagatc aacacatcca gtggcgattt
    34021 atgatcgacg atgatcaagc gcacaatggc ggtgatccca atatagacaa agtaacgtag
    34081 cgggaagtga aaaccggact gaaagtactt cacaatcagc gcgataaatt cgaaatagag
    34141 aaagtaaacc accagtcctt ctaccagctc atatttgctg gtttgttctg gcgcgaacag
    34201 cacatcagcc agatgcaccg tttctttgcc gaggaagaca accaaaatca ggccgaggca
    34261 cagcaggcca agattgagta cggtctgcaa aatggtggag ataaactcca cgcgcggacg
    34321 agagagtgac gtcatagcag tacctccttc acagtggcga gcttcctgta taacgcaaaa
    34381 atgtgacgga gatctatatt ttttgtgtat gttttgttca acctggattt ggagcgcgcc
    34441 tccaccaaag ccggatatac ctacccgtag gccggataag ccgcgcctgg cggcgcatcc
    34501 ggcaagacaa accgcaccaa agccggtaat tcaaaaatca cccattcagg tgattcccag
    34561 ccaccagcaa tgcgcgaaaa atcgacaatg ttcctctgga acaaatgaaa cgtaacctga
    34621 taaaggtggt aaaaatgaag ccttattttg ctgctttgat gttatcagtc tctgttcttc
    34681 ctgcttatgc tggtccgctc ggtactgctg ataaagccga cctaccgcaa agcaatgtct
    34741 ccagcccaat gatggcccag tcgctcaggc aaccagacct gcaaccgata tcgacggaca
    34801 ggaagacaga atgttttcgc ctttacacac cagatcgtaa accgggggtg aactgcgttc
    34861 ctgatggctc aacaggtcat taagaatgag ggtgccagtg agtgcgaaaa ctggcacctt
    34921 tcccatgact tagcggaaat ttacgctacg gtcgctggtc atgtcataca actggaactt
    34981 acgcccaagt tgctgaccac cgtcacgcgt cagcggcgtc cagctaatcg ccgcacggct
    35041 gcgtgtcggg ccagacgaga agagatccaa cggtaccgag acatacacgc ctttggtgaa
    35101 gtccccttcg ccgtactcct ctttcgaaac gttggtgatc gtggcatagc cacccaccac
    35161 cacgccgctg tcaaagcgtt tagcgatctc cagcgtgccg cctttatccc ctgccagata
    35221 ctgcccgacg ctggctttca ccaacacgtc ctgggcgaaa gatggcgtcc agtaggcggt
    35281 cagatgtccg gttttcacgc tgtagtcggt gaatttcatc atatcttttg cactacgcca
    35341 gtcgcgctgt ttaacgtagt tggcatccag accaaacgcc cagttactgt ccagcggacg
    35401 atacaacact tcggcccccg caccgccaaa catggtttcg agataacctc catagacctg
    35461 accgtagaag ccgttgccca gatgctggaa gtagttggct tgcaggttat tcacatagac
    35521 atcgttctgc acatactcgc gcacatgggt acgaacgcgc ggtaagtgcg agtcctgcgg
    35581 tggattggtg tagttaaact tgtcgtagtt gttggcaaga tttgcaaaca ggctgccggt
    35641 ggtcagtaga tggtccgtca gccacaaatc tgccgttccc atcacgccca gctgatacat
    35701 gtaaaagttt tccgggccac cgaccgactg gttcagcacc ggatcgatat ggaaatcaaa
    35761 gcgtgatttg tcgatatacc agccctgctc ggtggacttc ggaaccactg gctcgacgcg
    35821 tttgtgcgcc agcgtcgttt cgtggcccaa cggctctccg gcgagatgat ttttcaggct
    35881 ggcgacatcg gtttccgtcg tcacttgcgg catgttaagg cgattttccg taatgcggat
    35941 cgttttgatc ccatccggca gatcgttcat cacgatccga ttagcacgga tgatcccttc
    36001 gcgcgaatca cgatatttca cctgctcgcc ggtaacatac agcgtatcgc ctttcgcctg
    36061 gatctgcgga tcggcaagtc cggcgttgta tttcagcaac gttaactgat tcgccaccac
    36121 cgaatgctgc aaaatggcat cctgcggctg cggttgatat tgcgggcggg cgttatcgat
    36181 gtaggacggg cgcagatcgt taaagttggt gcgcagcgtg acgccaaaca taaaggtgtt
    36241 gccacgttca tagctaaggt taacgtcggc ccaatcggta acgcgataaa tcgccccgac
    36301 gttaaactta cttttttgct ccagcttccc ggcaaaatcc tgctgataat tattaccttc
    36361 atactccagt ttcagacgca gcggttgcca gggcgtctgg tattccacgc cgccaaacag
    36421 tgaggcagga ccgtggaaca tctggctacc gtcgatagat cccgcctgtt tgtagctgtt
    36481 atcgcgatag caatatttat cactggctga acagagcgga ttttttacat taccgctggt
    36541 gcccagatat ccccagccca ggccgagcgt aaaatcgaac ggcccccagg ctttgctggc
    36601 aacaagatat tccgcatcaa acagccccgt accgccgata tcccgcgcac caaccgccac
    36661 ttgcggcagc cagtaactct cttcccacaa acgcagtttg agatcgaagg ctttatcttt
    36721 atacgtttga tcgccagaga acgcttcgac gctgctgtac tgccgggtgc gcacgtcggt
    36781 gtagcgcagc gttgtttcca gccacgggaa gagttgcact gaagctgagt aataacggta
    36841 ctgatcgtta tcgcgatagt tcagactcaa ctccccttcc cgtgccatac gcgcggtggg
    36901 agtttgtaat aatcctacgc caccgaaatc cgactgcgac ggaccaatgg gcgcaggata
    36961 tgtttcgccg tagcaagctg tgctaatgcc cagcgccagt aagctaagca gatgtctttt
    37021 tttcattatt ggggtatccg ctgcgtcaga gtctgaagaa tgtcggcgtt aagggcatca
    37081 ggcgtctcac tccagacgga atctgcgagg ccaacataaa taatgctgcc gggcatcggc
    37141 tctacgtgac gcttgttcca gtaagccacc ggcgcttttt gcgtgcgtcc gtccgggtaa
    37201 acgacccacg cgtagctgcg atccgcaccg ctgagcaggc tctggtcaga gagatagctc
    37261 gccacgtcgc gacctggagt gaatggctga ttgccaggat ggctgataag cccgaacaac
    37321 gtgaccgtgg acggcggtgg tccgacccac agcgtgtagt tgccctgcaa cggcgggtta
    37381 ccgcgttcgg caacgcggac gatatcggga tcaagattga ttttttgcct gcctgtcact
    37441 ttcaatgcct ggatttgctg gcgtaaggcg ttaatggcgg cggcatcgtc ggtgctggaa
    37501 tctgcgccct gttctgccag ccgtgtcagc aacgcctgct gctgacgcaa tgctgccgcc
    37561 gttgccagct cttcgctaat caccgcacca ggccaccagc tgtttgccag tcgaggttgc
    37621 cctaccagat cgattaaatg ctctgcgccc gttagcgttt tggcctcact gctgccattg
    37681 ctgaacacct tgacggttcc tgccgcgaag accgatgacg ctcccacact caaaagcaac
    37741 gcgacaatag tttgtttaat cattgttttg ccgccttgat gagagtggtt ttcaccggga
    37801 aataatccgc gccgagatac tgttccgact ggcgaatttg cccttcgctg tcgatccagt
    37861 agcggttatg ccagcgcgcc tggtcggtgg agacttcttc gtccagcacg cgaaccaggg
    37921 tttcatcgct gccgaccttg acggtatcgg tgccatccca tttgaagact gagcgtgcgg
    37981 tggcgtagcg tacctgctgg tactcggtcc agcccatcgt gcgcgtccat gttgcaccat
    38041 caacaatttg tgcgggttta atcagcgggt cggcggcaag gttattcact tcaatcaggt
    38101 tgtcgccgcc aagcaaagtc ttcaccagac gaccatgttg tgtgacaaga gtggcttgat
    38161 cctgagtgac ccatttttgt tgtccgtctt cagcgaaggc cagcaccaca aacaattgcg
    38221 gcccgccatt aagctgcatg tactggctgg cgtagggcat attttgaata tcatcgtccg
    38281 tcagctgtac gcctggcgtg ccgaacagac tgtcccacag tgaattgccc agctctttag
    38341 tcgtggctga acaggcctgt aatagcaggc agataagaat gagtgcaggt cgcttcacga
    38401 ctcttctcca gaggggcaga ataaccacac cggagtgtgg ttatggtaaa tcccttcata
    38461 cttcaagcca cagatgcaat gactcaaatt attttgggga tacatacttt attactgggt
    38521 actggtggtc gtggttgtgg tggtcccggt attagaacca tcaccgccac cggttgccgc
    38581 gagcgcgaca cccaccgccg agcttacggt gctgacgctg gtcgcggagg aactccccgc
    38641 cgaaaccgac gttgctgccg actctgccgc ttcgcccacc tgtacaggtg cagcccacgc
    38701 agaagtcgcc gcaagcgcag atatggcaaa aatgccatac agaacttttt tcataacaat
    38761 ttcccttcat tgaatgaatg gagatttacc caaaaacatt tcgggcggga tcgagtatac
    38821 acaactaaag catgcgaatt acggcgcggg gaattagcga tggagtttta ggataattgg
    38881 aatgaatatc aaatataagt ttcaacttat atttaaataa caataaaaat cataagctta
    38941 aaataaaaaa caaaagtaat taatctgcgt attctccgaa ataaacaacg attaaaaatc
    39001 tattttagga ttaacctcac ggtatgattt ccgttaaatc acagacaagg ggatttatct
    39061 gataaaacaa tgccgactta ttgtcggcat tgtttttaag gttatttaac tcctcatact
    39121 atgcattaat gtcgatattg gcatcaggct ccggttgccg gatgcggcgt aaacgcctta
    39181 tccggcctac atatcggcat caggcacgca tgatgattaa ccgcgccacg ctttatagcg
    39241 gttaatcaga ccattggtcg agctatcgtg gctgctgatt tctttatcat ctttcagctc
    39301 tggcagaata cggtttgcca gttgtttacc cagctccacg ccccactggt cgaaggtgaa
    39361 gatgttcagg atcacgccct gagtaaagat tttgtgctca tacagcgcaa tcaacgcacc
    39421 caggctgaac ggagtgattt cgcgcagcag gatggagttg gtcgggcggt taccttcgaa
    39481 cactttgaac ggcaccacgt agtcaagcgt tgccggatct ttaccctgat cgcgatattc
    39541 ctgctcaacc acttcgcggg atttaccaaa cgccagcgct tcggtctggg cgaagaagtt
    39601 agacagcagt ttctggtggt gatcagagag cgggttatgg gtgatagccg gggcgatgaa
    39661 atcgcacggt accattttgg ttccctggtg gatcagctgg tagaacgcgt gctgaccgtt
    39721 agtgcctggt tcaccccaga taatcgggcc agtctggtaa tccacaacgt taccgttacg
    39781 gtcaacatac ttaccgttgg actccatatt gccctgctgg aagtacgccg cgaaacggtg
    39841 catatactgg tcatacggca gaatcgcttc agtttccgca ccaaagaaat tgttgtacca
    39901 gatgccaatc agcgccagca ggacaggcag gtttttctcg gcaggcgtgg tggagaaatg
    39961 cttgtccatt gcgtgtgcgc cagaaagcag ttcaacgaag ttatcaaagc cgatggagag
    40021 aacaatcgac aggccaatcg ctgaccacaa agagtaacgg ccgccgaccc agtcccagaa
    40081 ctcgaacatg ttggcagtat caataccaaa ctcgccaacg gctttggcat tggttgagag
    40141 cgccgcaaaa tgtttcgcaa cgtgtttttc atcacctgcc gctttcagga accagtcacg
    40201 cgcgctatgg gcgttggtca tggtttcctg agtggtgaag gttttagatg ctaccaggaa
    40261 cagcgtggtt tccgggttta cttttttcag cacttccgag atgtgagtcc catcgacgtt
    40321 agaaacaaag tgcatgttca ggtggttttt gtacggacgc agtgcttcgg tcaccatgta
    40381 cgggccgagg tcagaaccgc cgatcccgat gttcactacg tcagtgattg ctttgccggt
    40441 ataacccttc cactcaccgg agataatcgc ttccgagaag gttttcatct tctccagcac
    40501 cgcgttgact tctggcataa catctttgcc atcaaccaga atcggggtat tgctacgatt
    40561 acgcagcgct acgtgcagca cggcgcggtt ttcagtgcgg ttgatcttct cgccagagaa
    40621 catcgactta atcgcgcccg ccagatcgca ctctttcgcc agatcctgta atttcgccag
    40681 cgtctcttca gtgatgcggt ttttggagta atccaccagc atctgatcgt cgaaggttgc
    40741 ggagaactta gaaaaacgat cgccgtcttt agcaaaaaga tcggcgatcg taacgtcttt
    40801 catttcatcg aagtgtttct gtagtgcctg ccaggcagcg gtctgcgttg gattgatgtt
    40861 tttcattagc aatactcttc tgattttgag aattgtgact ttggaagatt gtagcgccag
    40921 tcacagaaaa atgtgatggt tttagtgccg ttagtgtaat gttgagtgta aacccttagc
    40981 gcagtgaagc atttattagc tgaactactg accgccagga gtggatgaaa aatccgcatg
    41041 accccgtcgt tgacaaccgc cccgctcacc ctttatttat aaatgtacta cctgcgctag
    41101 cgcaggccag aagaggcgcg ttgcccaagt aacggtgttg gaggagccag tcctgtgata
    41161 acacctgagg gggtgcatcg ccgaggtgat tgaacggctg gccacgttca tcatcggcta
    41221 caggggctga atcccctggg ttgtcaccag aagcgttcgc agtcgggcgt ttcgcaagtg
    41281 gtggagcact tctgggtgaa aatagtagcg aagtatcgct ctgcgcccac ccgtcttccg
    41341 ctcttccctt gtgccaaggc tgaaaatgga tcccctgaca cgaggtagtt atgtctgaaa
    41401 ttgttgtctc caaatttggc ggtaccagcg tagctgattt tgacgccatg aaccgcagcg
    41461 ctgatattgt gctttctgat gccaacgtgc gtttagtagt cctctcggct tctgctggta
    41521 tcactaatct gctggtcgct ttagcggaag ggctggaacc tggcgagcga ttcgaaaaac
    41581 tcgacgctat tcgcaacatc cagtttgcca ttctggaacg tctgcgttac ccgaacgtta
    41641 tccgtgaaga gattgaacgt ctgctggaga acattactgt tctggcagaa gcggcggcgc
    41701 tggcaacgtc tccggcgctg acagatgagc tggtcagcca cggcgagctg atgtcgaccc
    41761 tgctgtttgt cgaaatcctg cgcgaacgcg atgttcaggc acagtggttt gatgtacgta
    41821 aagtgatgcg taccaacgac cgatttggtc gtgcagagcc agatgtagcc gcgctggcgg
    41881 aactggccgc gctgcagctg ctcccacgcc tcaatgaagg cttagtgatc acccagggat
    41941 ttatcggtag cgaaaataaa ggtcgtacaa cgacgcttgg ccgtggaggc agcgattata
    42001 cggcagcctt gctggcggag gctttacacg catctcgtgt tgatatctgg accgacgtcc
    42061 cgggcatcta caccaccgat ccacgcgtag tttccgcagc aaaacgcatt gatgaaatcg
    42121 cgtttgccga agcggcagag atggcaactt ttggtgcaaa agtactgcat ccggcaacgt
    42181 tgctacccgc agtacgcagc gatatcccgg tctttgtcgg ctccagcaaa gacccacgcg
    42241 caggtggtac gctggtgtgc aataaaactg aaaatccgcc gctgttccgc gcgctggcgc
    42301 ttcgtcgcaa tcagactctg ctcactttgc acagcctgaa tatgctgcat tctcgcggct
    42361 tcctcgcgga agttttcggc atcctcgcgc ggcataatat ttcggtagac ttaatcacca
    42421 cgtcagaagt gagcgtggca ttaacccttg ataccaccgg ttcaacctcc actggcgata
    42481 cgttgctgac gcaatctctg ctgatggagc tttccgcact gtgccgggtg gaggtggaag
    42541 aaggtctggc gctggtcgcg ttgattggca atgacctgtc aaaagcctgc ggcgttggca
    42601 aagaggtatt cggcgtactg gaaccgttca acattcgcat gatttgttac ggcgcat
//
LOCUS       DAJTRC010000025        39990 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.025, whole genome shotgun sequence.
ACCESSION   DAJTRC010000025 DAJTRC010000000
VERSION     DAJTRC010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39990)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39990)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39990
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.025"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..155
                     /locus_tag="OO357_001460"
     CDS             <1..155
                     /locus_tag="OO357_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5886456.1"
                     /translation="EQKMLQHTPIRRLGQPQDIANAALFLCSPAASWVSGQILTVSGG
                     GVQELN"
     gene            384..974
                     /gene="uidR"
                     /locus_tag="OO357_001461"
     CDS             384..974
                     /gene="uidR"
                     /locus_tag="OO357_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator UidR"
                     /protein_id="HCR5886457.1"
                     /translation="MMDNMQTEAQPTRTRILNAAREIFSENGFHSASMKAICKSCAIS
                     PGTLYHHFISKEALIQAIILQDQERALARFREPIEGIHFVDYMVESIVSLTHEAFGQR
                     ALVVEIMAEGMRNPQVAAMLKNKHMTITEFVAQRMRDAQQKGEISPDINTAMTSRLLL
                     DLTYGVLADIEAEDLAREASFAQGLRAMIGGILTAS"
     gene            1362..3065
                     /gene="uidA"
                     /locus_tag="OO357_001462"
     CDS             1362..3065
                     /gene="uidA"
                     /locus_tag="OO357_001462"
                     /EC_number="3.2.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-glucuronidase"
                     /protein_id="HCR5886458.1"
                     /translation="MLRPVETPTREIKKLDGLWAFSLDRENCGIDQRWCESALQESRA
                     IAVPGSFNDQFADADIRNYVGNVWYQREVFIPKGWAGQRIVLRFDAVTHYGKVWVNNQ
                     EVMEHQGGYTPFEADVTPYVIAGKSVRITVCVNNELNWQTIPPGMVITDENGKKKQSY
                     FHDFFNYAGIHRSVMLYTTPNTWVDDITVVTHVAQDCNHASVDWQVVANGDISVELRD
                     ADQQVVATGQGTSGTLQVVNPHLWQPGEGYLYELCVTAKSQTECDIYPLRVGIRSVAV
                     KGEQFLINHKPFYFTGFGRHEDADLRGKGFDNVLMVHDHALMDWIGANSYRTSHYPYA
                     EEMLDWADEHGIVVIDETAAVGFNLSLGIGFEAGNKPKELYSEEAVNGETQQAHLQAI
                     KELIARDKNHPSVVMWSIANEPDTRPQGAREYFAPLAEATRKLDPTRPITCVNVMFCD
                     AHTDTISDLFDVLCLNRYYGWYVQSGDLETAEKVLEKELLAWQEKLHQPIIITEYGVD
                     TLAGLHSMYTDMWSEEYQCAWLDMYHRVFDRVSAVVGEQVWNFADFATSQGILRVGGN
                     N"
     gene            3071..4096
                     /gene="uidB"
                     /locus_tag="OO357_001463"
                     /pseudo
     CDS             3071..4096
                     /gene="uidB"
                     /locus_tag="OO357_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002431055.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide transporter"
     gene            4135..5400
                     /gene="uidC"
                     /locus_tag="OO357_001464"
     CDS             4135..5400
                     /gene="uidC"
                     /locus_tag="OO357_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronide uptake porin UidC"
                     /protein_id="HCR5886459.1"
                     /translation="MRKIVAMAVICLTAASGLTSAYAAQLADDEAGLRIRLKNELRRA
                     DKPSAGAGRDIYAWVQGGLLDFNSGYYSNIVGVEGGAYYVYKLGARADMSTRWYLDGD
                     KSFGFALGAVKIKPSENSLLKLGRFGTDYSYGSLPYRIPLMVGSSQRTLPTVSEGALG
                     YWALTPNIDLWGMWRSRVFLWTDSTTGIRDEGVYNSQTGKYDKHRARSFLAASWYDDT
                     SRYSLGASVQKDVSNQIQSILEKSIPLDPNYTLKGELLGFYAQLEGLSRNTSQPNETA
                     LVSGQLTWNAPWGSVFGSGGYLRHAMNGAVVDTDIGYPFSLSLDRNREGMQSWQLGAN
                     YRVTPQFTLTFAPIVTRGYESSKRDVRIEGAGILGGMNYRVSEGPLQGMNFFLAADKG
                     REKRDGSTLGDRLNYWDVKMSIQYDFMLK"
     gene            complement(5626..7134)
                     /locus_tag="OO357_001465"
     CDS             complement(5626..7134)
                     /locus_tag="OO357_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5886460.1"
                     /translation="MNKSLVAVGVIVALGVVWTGGAWYTGKKIETHLEDMVAQANAQL
                     KLTAPESNLEVSYQNYHRGVFSSQLQLLVKPIAGKENPWIKSGQSVIFNESVDHGPFP
                     LAQLKKLNLIPSMASIQTTLVNNEVSKPLFDMAKGETPFEINSRIGYSGDSSSDISLN
                     PLNYEQKDEKVAFSGGEFQLNADRDGKAISLSGEAQSGRIDAVNEYNQKVQLTFNNLK
                     TDGSSTLGSFGERVGNQKLSLEKMTISVEGKELALLEGMEISGKSDLVNDGKTINSQL
                     DYSLNSLKVQNQDLGSGKLTLKVGQIDGEAWHQFSQQYNAQTQALLAQPEIANNPELY
                     QEKVTEAFFSALPLMLKGDPVITIAPLSWKNSQGESALNLSLFLKDPATTKEAPQTLA
                     QEVDRSVKSLDAKLTIPVDMATELMTQVAKLEGYQEDQAKKLAKQQVEGASAMGQMFR
                     LTTLQDNTITTSLQYANGQITLNGQKMSLEDFVGMFAMPALNVPAVPAIPQQ"
     gene            complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OO357_001466"
     CDS             complement(7235..8410)
                     /gene="manA"
                     /locus_tag="OO357_001466"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HCR5886461.1"
                     /translation="MQKLINSVQNYAWGSKTALTELYGMENPSSQPMAELWMGAHPKS
                     SSRVQNAAGDIVSLRDVIESDKSTLLGEAVAKRFGELPFLFKVLCAAQPLSIQVHPNK
                     HNSEIGFAKENAAGIPMDAAERNYKDPNHKPELVFALTPFLAMNAFREFSEIVSLLQP
                     VAGAHPAIAHFLQQPHAERLSELFANLLNMQGEEKSRALAILKSALDSQQGEPWQTIR
                     LISEFYPEDSGLFSPLLLNVVKLNPGEAMFLFAETPHAYLQGVALEVMANSDNVLRAG
                     LTPKYIDIPELVANVKFEAKPANQLLTQPVKQGAELDFPIPVDDFAFSLHDLSDKETT
                     ISQQSAAILFCVEGDATLWKGSQQLQLKPGESAFIAANESPVTVKGHGRLARVYNKL"
     gene            8609..10255
                     /gene="fumB"
                     /locus_tag="OO357_001467"
     CDS             8609..10255
                     /gene="fumB"
                     /locus_tag="OO357_001467"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000066594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5886462.1"
                     /translation="MSNKPFHYQSPFPLKKNDTEYYLLTSEHVSVSEFEGQEILKVAP
                     EALTLLARQAFHDASFMLRPAHQQQVADILRDPEASENDKYVALQFLRNSDIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEAALARGVYNTYIEDNLRYSQNAPLDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCIAKGGGSANKTYLYQETKALLTPGKLKNYLVEKMRT
                     LGTAACPPYHIAFVIGGTSAETNLKTVKLASAKYYDELPTEGNEHGQAFRDVELEKEL
                     LIEAQNLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINRQGIW
                     IEKLEHNPGKYIPEELRKAGEGEAVRVDLNRPMKEILAQLSQYPVSTRLSLNGTIIVG
                     RDIAHAKLKERMDNGEGLPQYIKDHPIYYAGPAKTPEGYASGSLGPTTAGRMDSYVDQ
                     LQAQGGSMIMLAKGNRSQQVTDACKKHGGFYLGSIGGPAAVLAQGSIKSLECVEYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIQLTQCTRCVK"
     gene            10398..11801
                     /gene="fumC"
                     /locus_tag="OO357_001468"
     CDS             10398..11801
                     /gene="fumC"
                     /locus_tag="OO357_001468"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HCR5886463.1"
                     /translation="MNTVRSEKDSMGAIDVPADKLWGAQTQRSLEHFRISTEKMPTSL
                     IHALALTKRAAAKVNEDLDLLSEEKASAIRQAADEVLAGQHDDEFPLAIWQTGSGTQS
                     NMNMNEVLANRASELLGGVRGMERKVHPNDDVNKSQSSNDVFPTAMHVAALLALRKQL
                     IPQLKTLTQTLSEKSRAFADIVKIGRTHLQDATPLTLGQEISGWVAMLEHNLKHIEYS
                     LPHVAELALGGTAVGTGLNTHPEYARRVADELAVITCAPFVTAPNKFEALATCDALVQ
                     AHGALKGLAASLMKIANDVRWLASGPRCGIGEISIPENEPGSSIMPGKVNPTQCEALT
                     MLCCQVMGNDVAINMGGASGNFELNVFRPMVIHNFLQSVRLLADGMESFNKHCAVGIE
                     PNRERINQLLNESLMLVTALNTHIGYDKAAEIAKKAHKEGLTLKAAALALGYLSEAEF
                     DSWVRPEQMVGSMKAGR"
     gene            complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OO357_001469"
     CDS             complement(11798..12727)
                     /gene="tus"
                     /locus_tag="OO357_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication terminus site-binding protein"
                     /protein_id="HCR5886464.1"
                     /translation="MARYDLVDRLNTTFRQMEQELATFAAHLEQHKLLVARVFSLPEV
                     KKEDEHNPLNRIEVKQHLGNDAQSLALRHFRHLFIQQQSENRSSKAAVRLPGVLCYQV
                     DNLSQAALVSHIQHINKLKTTFEHIVTVESELPTAARFEWVHRHLPGLITLNAYRTLT
                     VLHDPATLRFGWANKHIIKNLHRDEVLAQLEKSLKSPRSVAPWTREEWQRKLEREYQD
                     IAALPQNAKLKIKRPVKVQPIARVWYKGDQKQVQHACPTPLIALINRDNGAGVPDVGE
                     LLNYDADNVQHRYKPQAQPLRLIIPRLHLYVAD"
     gene            complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OO357_001470"
     CDS             complement(12803..14104)
                     /gene="rstB"
                     /locus_tag="OO357_001470"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     RstB"
                     /protein_id="HCR5886465.1"
                     /translation="MKKLFIQFYLLLFVCFLVMSLLVGLVYKFTAERAGKQSLDDLMN
                     SSLYLMRSELREIPPHDWGKTLKEMDLNLSFDLRVEPLSKYHLDDISMHRLRGGEIVA
                     LDDQYTFLQRIPRSHYVLAVGPVPYLYYLHQMRLLDIALIAFIAISLAFPVFIWMRPH
                     WQDMLKLEAAAQRFGDGHLNERIHFDEGSSFERLGIAFNQMADNINALIASKKQLIDG
                     IAHELRTPLVRLRYRLEMSDNLSAAESQALNRDISQLEALIEELLTYARLDRPQNELH
                     LSEPDLPLWLSTHLADIQAVTPDKTVRIKTLMQGHYAALDMRLMERVLDNLLNNALRY
                     CHSTVETSLLLSGNRATLIVEDDGPGIAPENREHIFEPFVRLDPSRDRSTGGCGLGLA
                     IVHSIALTMGGTVNCDISELGGARFSFSWPLWHNIPQFTSA"
     gene            complement(14283..15602)
                     /locus_tag="OO357_001471"
     CDS             complement(14283..15602)
                     /locus_tag="OO357_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707507.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:dicarboxylase symporter family
                     transporter"
                     /protein_id="HCR5886466.1"
                     /translation="MLFLICSWGIFKTDQKSESLSKSVFIAIFLGAIIGISLHFIPET
                     SSETVIEWYNIAGNGYVNLLKLVAVPLIFISILSAINKLENSAGIGKMSLTIVACMLC
                     LVMIAGLVGLAIASAIGLDASAFGHMQSSLTSDDVSKTAAVSLPRLITSLIPTNLFLD
                     LTGARSVSVIGIVIFTILAGIALLKVKKDAPAEGEKLSAGINAIQIWVMKMVRIVIAL
                     TPYGVMALMAKVFSTYQLSQFTNLLGFIVACYLAVFVMFIVHSGILLLIGKNPVNYYK
                     TVWQVLTFAFVSRSSAASIPLAISALEKLGVPNTIANIAASFGSSMGQNGCAGIYPAV
                     MVAMIAPTMGIDPFSLQFLATLLPAIALGSIGVAGVGSGGTFAALIVLSTLNFPVALV
                     VIFIAIEPIVDMARTALNVNGSIMSGVISARLLKDRETKEQQSVTLN"
     gene            complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OO357_001472"
     CDS             complement(15827..16570)
                     /gene="rstA"
                     /locus_tag="OO357_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator RstA"
                     /protein_id="HCR5886467.1"
                     /translation="MNTIVFVEDDAEVGSLIAAYLAKHDMQVTVEPRGDQAEETILRE
                     NPDLVLLDIMLPGKDGMTICRDLRAKWSGPIVLLTSLDSDMNHILALEMGACDYILKT
                     TPPAVLLARLRLHLRQNEQATLTKGLQETSLTPYKALHFGTLTIDPINRVVTLANTEI
                     SLSTADFELLWELATHAGQIMDRDALLKNLRGVSYDGLDRSVDVAISRLRKKLLDNAA
                     EPYRIKTVRNKGYLFAPHAWDSDNKQSDD"
     gene            16699..17034
                     /locus_tag="OO357_001473"
     CDS             16699..17034
                     /locus_tag="OO357_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlpM family protein"
                     /protein_id="HCR5886468.1"
                     /translation="MGLVIKAALGALVVLLIGVLAKTKNYYIAGLIPLFPTFALIAHY
                     IVASERGIEALRATIIFSMWSIIPYFVYLVSLWYFTGMMRLPAAFVGSVACWGISAWV
                     LIICWIKLH"
     gene            complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OO357_001474"
     CDS             complement(17031..17753)
                     /gene="folM"
                     /locus_tag="OO357_001474"
                     /EC_number="1.5.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydromonapterin reductase"
                     /protein_id="HCR5886469.1"
                     /translation="MGKTQPLPILITGGGRRIGLALAWHFINQKQPVIVSYRTHYPAI
                     DGLINAGAQCIQADFSTNDGVMAFADEVLKTTHGLRAILHNASAWIAEKPGAPLADVL
                     ACMMQIHVNTPYLLNHALERLLRGHGHAASDIIHFTDYVVERGSDKHVAYAASKAALD
                     NMTRSFARKLAPEVKVNSIAPSLILFNEHDDAEYRQQALNKSLMKTAPGEKEVIDLVD
                     YLLTSCFVTGRSFPLDGGRHLR"
     gene            complement(17790..19172)
                     /locus_tag="OO357_001475"
     CDS             complement(17790..19172)
                     /locus_tag="OO357_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5886470.1"
                     /translation="MEKKLGLSALTALVLSSMLGAGVFSLPQNMAAVASPAALLIGWG
                     ITGAGILLLAFAMLILTRIRPELDGGIFTYAREGFGELIGFCSAWGYWLCAVIANVSY
                     LVIVFSALSFFTDTPELRLFGDGNTWQSIVGASALLWIVHFLILRGVQTAASINLVAT
                     LAKLLPLGLFVVLAMMMFKLDTFKLDFTGLALGVPVWEQVKNTMLITLWVFIGVEGAV
                     VVSARARNKRDVGKATLLAVLSALGVYLLVTLLSLGVVARPELAEIRNPSMAGLMVEM
                     MGPWGEIIIAAGLIVSVCGAYLSWTIMAAEVPFLAATHKAFPRIFARQNAQAAPSASL
                     WLTNICVQICLVLIWLTGSDYNTLLTIASEMILVPYFLVGAFLLKIATRPLHKAVGVG
                     ACIYGLWLLYASGPMHLLLSVVLYAPGLLVFLYARKTHTHDNVLNRQEMVLIGMLLIA
                     SVPATWMLVG"
     gene            complement(19358..20302)
                     /locus_tag="OO357_001476"
     CDS             complement(19358..20302)
                     /locus_tag="OO357_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5886471.1"
                     /translation="MKLKNTLLASALLSATAFSVNAATELTPEQAAAVKPFDRVVVTG
                     RFNAIGEAVKAVSRRADKEGAASFYVVDTSDFGNSGNWRVVADLYKADAEKAEETSNR
                     VINGVVELPKDQAVLIEPFDTVTVQGFYRSQPEVNDAITKAAKAKGAYSFYIVRQIDA
                     NQGGNQRITAFIYKKDAKKRIVQSPDVIPADSEAGRAALAAGGEAAKKVEIPGVATTA
                     SPSSEVGRFFETQSSKGGRYTVTLPDGTKVEELNKATAAMMVPFDSIKFSGNYGNMTE
                     VSYQVAKRAAKKGAKYYHITRQWQKRGNNLTVSADLYK"
     gene            20826..22358
                     /gene="pntA"
                     /locus_tag="OO357_001477"
     CDS             20826..22358
                     /gene="pntA"
                     /locus_tag="OO357_001477"
                     /EC_number="1.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit alpha"
                     /protein_id="HCR5886472.1"
                     /translation="MRIGIPRERLTNETRVAATPKTVEQLLKLGFTVAVESGAGQLAS
                     FDDKAFVQAGAEIVEGNSVWQSEIILKVNAPLDDEIALLNPGTTLVSFIWPAQNPELM
                     QKLAERNVTVMAMDSVPRISRAQSLDALSSMANIAGYRAIVEAAHEFGRFFTGQITAA
                     GKVPPAKVMVIGAGVAGLAAIGAANSLGAIVRAFDTRPEVKEQVQSMGAEFLELDFKE
                     EAGSGDGYAKVMSDAFIKAEMELFAAQAKEVDIIVTTALIPGKPAPKLITREMVDSMK
                     AGSVIVDLAAQNGGNCEYIVPGEIFSTENGVKVIGYTDLPGRLPTQSSQLYGTNLVNL
                     LKLLCKEKDGNITVDFDDVVIRGVTVIRAGEITWPAPPIQVSAQPQAAQKAAPEVKTE
                     EKCACSPWRKYALMALAIILFGWLSSVAPKEFLGHFTVFALACVVGYYVVWNVSHALH
                     TPLMSVTNAISGIIVVGALLQIGQGGWVSFLSFIAVLIASINIFGGFTVTQRMLKMFR
                     KN"
     gene            22369..23757
                     /gene="pntB"
                     /locus_tag="OO357_001478"
     CDS             22369..23757
                     /gene="pntB"
                     /locus_tag="OO357_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000014033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Re/Si-specific NAD(P)(+) transhydrogenase
                     subunit beta"
                     /protein_id="HCR5886473.1"
                     /translation="MSGGLVTAAYIVAAILFIFSLAGLSKHETSRQGNNFGIAGMAIA
                     LIATIFGPDTGNVGWILLAMVIGGAIGIRLAKKVEMTEMPELVAILHSFVGLAAVLVG
                     FNSYLHHDAGMAPILVNIHLTEVFLGIFIGAVTFTGSVVAFGKLCGKISSKPLMLPNR
                     HKMNLAALVVSFLLLIVFVRTDSVGLQVLALLIMTAIALVFGWHLVASIGGADMPVVV
                     SMLNSYSGWAAAAAGFMLSNDLLIVTGALVGSSGAILSYIMCKAMNRSFISVIAGGFG
                     TDGSSTGDDQEVGEHREITAEETAELLKNSHSVIITPGYGMAVAQAQYPVAEITEKLR
                     ARGINVRFGIHPVAGRLPGHMNVLLAEAKVPYDIVLEMDEINDDFADTDTVLVIGAND
                     TVNPAAQDDPKSPIAGMPVLEVWKAQNVIVFKRSMNTGYAGVQNPLFFKENTHMLFGD
                     AKASVDAILKAL"
     gene            complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OO357_001479"
     CDS             complement(23782..24816)
                     /gene="tqsA"
                     /locus_tag="OO357_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2 transporter TqsA"
                     /protein_id="HCR5886474.1"
                     /translation="MAKPIITLNGLKIVIMLGMLVIILCGIRFAAEIIVPFILALFIA
                     VILNPLVQHMVRWRVPRVLAVSILMTIIVMAMVLLLAYLGSALNELTRTLPQYRNSIM
                     TPLQALEPLLQRVGIDVSVDQLAHYIDPNAAMTLLTNLLTQLSNAMSSIFLLLLTVLF
                     MLLEVPQLPGKFQQMMARPVEGMAAIQRAIDSVSHYLVLKTAISIITGLVAWAMLAAL
                     DVRFAFVWGLLAFALNYIPNIGSVLAAIPPIAQVLVFNGFYEALLVLAGYLLINLVFG
                     NILEPRIMGRGLGLSTLVVFLSLIFWGWLLGPVGMLLSVPLTIIVKIALEQTAGGQSI
                     AVLLSDLNKE"
     gene            25227..25592
                     /gene="mdtJ"
                     /locus_tag="OO357_001480"
     CDS             25227..25592
                     /gene="mdtJ"
                     /locus_tag="OO357_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtJ"
                     /protein_id="HCR5886475.1"
                     /translation="MYIYWILLGLAIATEITGTLSMKWASVSEGNGGFILMLVMISLS
                     YIFLSFAVKKIALGVAYALWEGIGILFITLFSVLLFDESLSLMKIAGLTTLVAGIVLI
                     KSGTRKARKPELEVNHGAV"
     gene            25579..25908
                     /gene="mdtI"
                     /locus_tag="OO357_001481"
     CDS             25579..25908
                     /gene="mdtI"
                     /locus_tag="OO357_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug/spermidine efflux SMR transporter
                     subunit MdtI"
                     /protein_id="HCR5886476.1"
                     /translation="MAQFEWVHAAWLALAIVLEIVANVFLKFSDGFRRKIFGLLSLAA
                     VLAAFSALSQAVKGIDLSVVYALWGGFGIAATLAAGWILFGQRLNRKGWIGLVLLLAG
                     MIMVKLA"
     gene            complement(25947..26768)
                     /locus_tag="OO357_001482"
     CDS             complement(25947..26768)
                     /locus_tag="OO357_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease"
                     /protein_id="HCR5886477.1"
                     /translation="MRTTIAVVLGAISLTSAFVFADKPDVAKSANDEVSTLFFGHDDR
                     VPVNDTTQSPWDAVGQLETASGNLCTATLIAPNLALTAGHCLLTPPKGKADKAVALRF
                     VSNKGLWRYEIHDIEGRVDPTLGKRLKADGDGWIVPPAAAPWDFGLIVLRNPPSGITP
                     LPLFEGDKAALTAALKSAGRKVTQAGYPEDHLDTLYSHQNCEVTGWAQTSVMSHQCDT
                     LPGDSGSPLMLHTDDGWQLIGVQSSAPAAKDRWRADNRAISVTGFRDKLDQLSQK"
     gene            complement(26868..26951)
                     /locus_tag="OO357_001483"
     CDS             complement(26868..26951)
                     /locus_tag="OO357_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886478.1"
                     /translation="MVGRYRFEFILIILILCALIAARFYLS"
     gene            complement(27044..27379)
                     /locus_tag="OO357_001484"
     CDS             complement(27044..27379)
                     /locus_tag="OO357_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid shock protein"
                     /protein_id="HCR5886479.1"
                     /translation="MKKQIEGMTMKKVLALVVAAAMGLSSAAFAAETATTPAPTATTT
                     KAAPAKTTHHKKQHKAAPAQKAQAAKKHHKNTKAEQKAPEQKAQAAKKHAGKHGHQQP
                     AKPAAQPAA"
     gene            complement(27776..29029)
                     /locus_tag="OO357_001485"
                     /pseudo
     CDS             complement(27776..29029)
                     /locus_tag="OO357_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416113.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            29136..30047
                     /locus_tag="OO357_001486"
     CDS             29136..30047
                     /locus_tag="OO357_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5886480.1"
                     /translation="MNIELRHLRYFVAVAEELHFGRAAARLNISQPPLSQQIQALEQQ
                     IGARLLARTNRSVLLTAAGKQFLADSRQILSMVDDAAARAERLHQGEAGELRIGFTSS
                     APFIRAVSDTLSLFRRDYPDVHLQTREMNTREQIAPLIEGTLDMGLLRNTALPETLEH
                     AVIVHEPLMAMIPYDHPLANNPSVTLAELAELAELAKEPFVFFDPHVGTGLYDDILGL
                     MRRYHLTPVITQEVGEAMTIIGLVSAGLGVSILPASFKRVQLNEMRWVPIAEEDAVSE
                     MWLVWPKHHEQSPAARNFRIHLLNALR"
     gene            30182..31402
                     /gene="mlc"
                     /locus_tag="OO357_001487"
                     /gene_synonym="dgsA"
     CDS             30182..31402
                     /gene="mlc"
                     /locus_tag="OO357_001487"
                     /gene_synonym="dgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor Mlc"
                     /protein_id="HCR5886481.1"
                     /translation="MVAENQPGHIDQIKQTNAGAVYRLIDQLGPVSRIDLSRLAQLAP
                     ASITKIVREMLEAHLVRELEIKEAGNRGRPAVGLVVETEAWHYLSLRISRGEIFLALR
                     DLSSKLVVEESQELALKDDLPLLDRIISHIDQFFIRHQKKLERLTSIAITLPGIIDTE
                     NGIVHRMPFYEDVKEMPLGEALEQHTGVPVYIQHDISAWTMAEALFGASRGARDVIQV
                     VIDHNVGVGVITDGHLLHAGSSSLVEIGHTQVDPYGKRCYCGNHGCLETIASVDSILE
                     LAQLRLNQSMSSMLHGQPLTVDSLCQAALRGDLLAKDIIIGVGAHVGRILAIMVNLFN
                     PQKILIGSPLSKAADILFPVISDSIRQQALPAYSQHISVESTQFSNQGTMAGAALVKD
                     AMYNGSLLIRLLQG"
     gene            31527..32222
                     /gene="bioD"
                     /locus_tag="OO357_001488"
     CDS             31527..32222
                     /gene="bioD"
                     /locus_tag="OO357_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5886482.1"
                     /translation="MLKRFFITGTDTSVGKTVVSRALLQALASQGKRVAGYKPVAKGS
                     KETPEGLRNKDALVLQSVSTIELPYEAVNPIALSEEESSVAHSCPINYTLISNGLANL
                     TDKVDHVVVEGTGGWRSLMNDLRPLSEWVVQEQLPVLMVVGIQEGCINHALLTAQAIA
                     NDGLPLIGWVANRINPGLAHYAEIIDVLGKKLPAPLIGELPYLPRAEQRELGQYIRLA
                     MLRSVLAVDRVTV"
     gene            complement(32175..33467)
                     /gene="clcB"
                     /locus_tag="OO357_001489"
     CDS             complement(32175..33467)
                     /gene="clcB"
                     /locus_tag="OO357_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001296088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated ClC-type chloride channel ClcB"
                     /protein_id="HCR5886483.1"
                     /translation="MHHLHIYPDLRTMFRRLLIATVVGILAVFAVAGFRHAMLLLEWL
                     FLNNDSGSLVNAATNLSSWRRLLTPALGGLAAGLLLMGWQKFTQQRPHAPTDYMEALQ
                     TDGQFDYAASLVKSLASLLVVTSGSAIGREGAMILLAALAASCFAQRFTPRQEWKLWI
                     ACGAAAGMAAAYRAPLAGSLFIAEVLFGTMMLASLGPVIISAIVAWLVSNLINHSDAL
                     LYNVQLSVTVQARDYALIISTGVLAGLCGPLLLTLMNACHRGFVSLKLAPPWQLALGG
                     LIVGLLSLFTPAVWGNGYSTVQSFLTAPPLLMIIAGIFLCKLCAVLASSGSGAPGGVF
                     TPTLFIGLAIGMLYGRSLGLWFPDGEEITLLLGLTGMATLLAATTHAPIMSTLMICEM
                     TGEYQLLPGLLIACVIASVISRTLHRDSIYRQHTAQHS"
     gene            complement(33626..34240)
                     /gene="dmsD"
                     /locus_tag="OO357_001490"
     CDS             complement(33626..34240)
                     /gene="dmsD"
                     /locus_tag="OO357_001490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707489.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tat proofreading chaperone DmsD"
                     /protein_id="HCR5886484.1"
                     /translation="MTHFSQQDNFSVAARVLGALFYYAPESAEAAPLVAVLTSDGWET
                     QWPLPEASLAPLVTAFQTQCEETHAQAWQRLFVGPWALPSPPWGSVWLDRESVLFGDS
                     TLALRQWMREKGIQFEMKQNEPEDHFGSLLLMAAWLAENGRQTECEELLAWHLFPWST
                     RFLDVFIEKAEHPFYRALGELARLTLAQWQSQLLIPVAVKPLFR"
     gene            complement(34283..35137)
                     /locus_tag="OO357_001491"
     CDS             complement(34283..35137)
                     /locus_tag="OO357_001491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5886485.1"
                     /translation="MGNGWHEWPLVIFTVLGQCVVGALIVSGIGWFAAKNDADRQHIV
                     RGMFFLWLLMGIGFIASVMHLGSPLRAFNSLNRIGASGLSNEIAAGSIFFAVGGLWWL
                     VAVIGKMPQALGKLWLLVSMALGVIFVWMMTCVYQIDTVPTWHNGYTTLAFFLTVLLS
                     GPILAAAILRAARVTFNTTPFAIISVLALIACAEVIVLQGLSLASIHSSVQQASALVP
                     DYASLQVWRVVLLCAGLGCWLCPLIRRREPHVAGLVLGLILILVGEMIGRVLFYGLHM
                     TVGMAIAG"
     gene            complement(35139..35756)
                     /gene="dmsB"
                     /locus_tag="OO357_001492"
     CDS             complement(35139..35756)
                     /gene="dmsB"
                     /locus_tag="OO357_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5886486.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDFKDLGPEVSFRRIYEYAG
                     GDWQEDNGIWHQNVFAYYLSISCNHCDDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNAEKGHMTKCDGCYSRVAEGKQPICVESCPLRALEFGPIEELRQ
                     KHGTLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPEEV"
     gene            complement(35767..37805)
                     /locus_tag="OO357_001493"
                     /pseudo
     CDS             complement(35767..37805)
                     /locus_tag="OO357_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416105.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(37866..38975)
                     /locus_tag="OO357_001494"
     CDS             complement(37866..38975)
                     /locus_tag="OO357_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000248656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5886487.1"
                     /translation="MINVSHIRTLSSIESTTSSPSIRLAMESIWNYAGNTLVNQHSDI
                     NKTHEILQDESKCEMIVVIENFMTSSAKYADILLEDLMTVEQEDIIPNDYAGNMGYLI
                     FLQPVTSEKFERKPIYWILSEVAKRLGPDVYQKFTEGRTQEQWLQHLYAKMLAKDPAL
                     PSYDELKKMGIYKRKDPNGHFVAYKAFRDDPEANPLKTPSGKIEIYSSKLAEIARTWE
                     LEKDEVISPLPVYASTFEGWDSPERRTFPLQLFGFHYKSRTHSTYGNIDVLKAACRQE
                     VWINPIDAQKRGIANGDMVRVFNHRGEVRLPAKVTPRILPGVSAMGQGAWHEPNMSGD
                     KIDHGGCVNTLTTLRPSPLAKGNPQHTNLVEIEKI"
     gene            38958..>39990
                     /locus_tag="OO357_001495"
     CDS             38958..>39990
                     /locus_tag="OO357_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5886488.1"
                     /translation="MTDINHDNTVYPSSSFSGSPYRYQQVYTSSPDTRLTQINLLSVA
                     KPQLVRRANGRYTIRFRLKGQTTPFLSVSTRSTDRRVATMRQRELAATAKAFMLDRPE
                     VSLQELTEHLRSMAEQFLTDASDDYWNGLEVATLVDEKSNLKELAATQALSLDQQKGI
                     RLALEVLTAAQQRVDTGDTSGLIKLIDDNNLTDDSTIGDSTSILNNEQGDRPAVFTQE
                     RQSSVVFSSLVSSLLAEKVQTLKTSSYKDLSSSLNTVSRFLPEDMDLMSRSGWLAVRD
                     SMLASEVRPSTINKLLTKAKMCLDYGLMNGQLEGRNPIERMKLTKDIDSKRRAFTDEE
                     LERLLVRVES"
BASE COUNT        10165 a         9700 c        10241 g         9884 t
ORIGIN      
        1 ttgaacaaaa aatgttacag cacacgccga tcagacgtct gggccaaccg caagatattg
       61 ctaacgcggc gctgttcctt tgctcgcctg cagccagctg ggtaagcgga caaattctca
      121 ccgtctccgg tggtggggta caggagctca attaatacac taacgaaccg gtaaacggtc
      181 gtatgtgttg tttaccggga tagcgtgtgc aattcatcaa cgtctctgct aaacaactgg
      241 cagccaaatc acggctattg gttaaccaat tgctgggtga aaagtatacg aatagagtgt
      301 accttcgcac tattcaacag caatgatagg cgctcacctg acaacgcggt aaactagtta
      361 ttcacgctaa ctataatggt ttaatgatgg ataacatgca gactgaagca caaccgacac
      421 ggacccggat cctcaatgct gccagagaga ttttttcaga aaatggattt cacagtgcct
      481 cgatgaaagc catctgtaaa tcttgcgcca ttagtcctgg gacgctctat caccatttca
      541 tctccaaaga agccttgatt caggcgatta tcttacagga ccaggagagg gcgctggctc
      601 gtttccggga accgattgaa gggattcatt ttgttgacta tatggtcgag tctattgtct
      661 ctctcaccca tgaagccttt gggcaacggg cgctggtggt cgaaattatg gcggaaggga
      721 tgcgtaaccc acaggtcgcc gccatgctta aaaataagca tatgacgatc actgaatttg
      781 ttgcccagcg gatgcgtgat gcccagcaaa aaggcgagat aagcccagac atcaacacgg
      841 caatgacttc acgtttactg ctggatctga cctacggtgt actggccgat atcgaagcgg
      901 aagacctggc gcgtgaagcg tcgtttgctc agggattacg cgcgatgatt ggcggtatat
      961 taaccgcatc ctgatcctct catttcgcgg gctggtgata actgtgcccg cgtttcatat
     1021 cgtaatttct ctgtgcaaaa attatcctcc ccgtgttcgg agaattcccc cccaaaatat
     1081 tcaccgtagc catatgtcat gagagtttat cgttcccaac actctcgaac gaacgttcga
     1141 ttgcttattt tatggcttct gtcaacgctg ttttaaagat taatgcgatc catatcacgc
     1201 tgtgggtatt gcagtttttg gttttttgat cgcggtgtca gttcttttta tttccatttc
     1261 tcttccatgg gtttctcaca gataactgtg tgcaacacag aattggttaa ctaatcagat
     1321 taaaggttga ccagtatttt tatcttaatg aggagtccct tatgttacgt cctgtagaaa
     1381 ccccaacccg tgaaatcaaa aaactcgacg gcctgtgggc attcagtctg gatcgcgaaa
     1441 actgtggaat tgatcagcgt tggtgcgaaa gcgcgttaca agaaagccgg gcaattgcag
     1501 tgccaggcag ttttaacgat cagttcgccg atgcagatat tcgtaattat gtgggcaacg
     1561 tctggtatca gcgcgaagtc tttataccga aaggttgggc aggccagcgt atcgtgctgc
     1621 gtttcgatgc ggtcactcat tacggcaaag tgtgggtcaa taatcaggaa gtgatggagc
     1681 atcagggcgg ctatacgcca tttgaagccg atgtcacgcc gtatgttatt gccgggaaaa
     1741 gtgtacgtat caccgtttgt gtgaacaacg aactgaactg gcagactatc ccgccgggaa
     1801 tggtgattac cgacgaaaac ggcaagaaaa agcagtctta cttccatgat ttctttaact
     1861 atgccgggat ccatcgcagc gtaatgctct acaccacgcc gaacacctgg gtggacgata
     1921 tcaccgtggt gacgcatgtc gcgcaagact gtaaccacgc gtctgttgac tggcaggtgg
     1981 tggccaatgg tgatatcagc gttgaactgc gtgatgcgga tcaacaggtg gttgcaactg
     2041 gacaaggcac cagcgggact ttgcaagtgg tgaatccgca cctctggcaa ccgggtgaag
     2101 gttatctcta tgaactgtgc gtcacagcca aaagccagac agagtgtgat atctacccgc
     2161 tgcgcgtcgg catccggtca gtggcagtga agggcgaaca gttcctgatt aaccacaaac
     2221 cgttctactt tactggcttt ggtcgtcatg aagatgcgga tttgcgaggc aaaggattcg
     2281 ataacgtgct gatggtgcac gatcacgcat taatggactg gattggggcc aactcctacc
     2341 gtacctcgca ttacccttac gctgaagaga tgctcgactg ggcagatgaa catggcatcg
     2401 tggtgattga tgaaactgca gctgtcggct ttaacctctc tttaggcatt ggtttcgaag
     2461 cgggcaacaa gccgaaagaa ctgtacagcg aagaggctgt caacggggaa actcagcagg
     2521 cgcacttaca ggcgattaaa gagctgatag cgcgtgacaa aaaccaccca agcgtggtga
     2581 tgtggagtat tgccaacgaa ccggataccc gtccgcaagg tgcacgggaa tatttcgcgc
     2641 cactggcgga agcaacgcgt aaactcgacc cgacgcgtcc gatcacctgc gtcaatgtaa
     2701 tgttctgcga cgctcacacc gataccatca gcgatctctt tgatgtgctg tgcctgaacc
     2761 gttattacgg ttggtatgtc caaagcggcg atttggaaac ggcagagaag gtactggaaa
     2821 aagaactgct ggcctggcag gagaaactgc atcagccgat tatcatcacc gaatacggcg
     2881 tggatacgtt agccggcctg cactcaatgt acaccgacat gtggagtgaa gagtatcagt
     2941 gtgcatggct ggatatgtat caccgcgtct ttgatcgcgt cagcgccgtc gtcggtgaac
     3001 aggtatggaa tttcgccgat tttgcgacct cgcaaggcat attgcgcgtt ggcggtaaca
     3061 attgacctac atgggcctcg ggctttgcta cagcctggtg aatattcctt atggttcact
     3121 tgctaccgcg atgacccaac aaccacaatc tcgcgcccgt ctgggcgcgg ctcgtgggat
     3181 tgccgcttca ttgacctttg tatgcctggc atttctgata ggaccgagca ttaagaactc
     3241 cagcccggaa gagatggtgt cggtatacca tttctggacg attgtgctgg tgattgccgg
     3301 aatggtgctt tacttcatct gcttcaaatc gacgcgtgaa aatgtggtac gtatcgtggc
     3361 gcagccgtca ttgaagatca gtttgcaaac cctgaaacgg aatcgcccgc tgtttatgtt
     3421 gtgcatcggt gcgctgtgtg tgctgatttc gaccttcgcg gtcagcgcct cgtcgttgtt
     3481 ctacgtgcgc tatgtgttaa atgataccgg gctgttcact gtgctggtac tggtgcaaaa
     3541 cctggttggt actgtggcat cggcaccgct tgtgccgggg atggtcgcga ggatcggtaa
     3601 aaagaatacc ttcctgattg gcgctttgct gggaacctgc ggttatctgc tattcttctg
     3661 ggtttccgtc tggtcactgc cggtggcgtt ggttgcgttg gccatcgctt caattggtca
     3721 gggcgttact atgactgtga tgtgggcact ggaagctgat accgtagaat acggtgaata
     3781 cctgaccggc gtgcgaattg aagggctcac ctattcacta ttctcattta cccgtaaatg
     3841 cggtcaggca atcggaggtt caattcctgc ctttattttg ggattaagcg gatatatcgc
     3901 caatcaggtg taaacgccgg aagttattat gggcatccgc acatcaattg ccttagtacc
     3961 ttgcggattt atgctactgg cattcgttat tatctggttt tatccgctca cggataaaaa
     4021 attcaaagaa atcgtggttg aaattgataa tcgtaaaaaa gtgcagcagc aattaatcag
     4081 cgatatcact aattaatatt caataaaaat aatcagaaca tcaaaggtgc aactatgaga
     4141 aaaatagtgg ccatggccgt tatttgcctg acggctgcct ctggccttac ctctgcttac
     4201 gcggcgcaac tggctgacga tgaagcggga ctacgcatca gactgaaaaa cgaattgcgc
     4261 agggctgata agcccagtgc tggcgcggga agagatattt acgcatgggt acagggagga
     4321 ttgctcgatt tcaatagtgg ttattattcc aatattgttg gcgttgaagg cggagcgtat
     4381 tatgtttata aattaggtgc tcgtgctgat atgagtaccc ggtggtatct tgatggtgat
     4441 aaaagttttg gctttgccct gggggcagta aaaataaaac ccagtgaaaa tagcctgctt
     4501 aaattaggtc gcttcgggac ggattatagt tatggtagct taccttatcg tattccgtta
     4561 atggttggca gttcgcaacg tacattaccg acagtttctg aaggagcatt aggttattgg
     4621 gctttaacgc caaatattga tctgtgggga atgtggcgtt cacgagtatt tttatggact
     4681 gattcaacaa ccggtattcg tgatgaaggg gtgtataaca gccagacggg aaaatacgat
     4741 aaacatcgcg cacgttcttt tttagccgcc agttggtatg atgataccag tcgctattct
     4801 ctgggggcat cggtacagaa agatgtttcc aatcagatac aaagtattct cgagaaaagc
     4861 ataccgctcg acccgaatta tacgttgaaa ggggagttgc tcggctttta cgcgcagctc
     4921 gaaggtttaa gtcgtaatac cagccagccc aatgaaacgg cgttggttag tggacaattg
     4981 acctggaatg cgccgtgggg aagcgtattt ggcagtggtg gttatttgcg ccatgcaatg
     5041 aatggtgccg tggtggatac cgacattggc tatccctttt cattaagtct tgatcgtaac
     5101 cgtgaaggaa tgcagtcctg gcaattaggt gccaactacc gtgtaactcc gcaatttacg
     5161 ctgacatttg caccgattgt gactcgcggc tatgaatcca gtaaacgaga tgtgcggatt
     5221 gaaggcgcgg gtatcttagg tggtatgaac tatcgggtca gcgaagggcc gttacaaggg
     5281 atgaatttct ttcttgctgc cgataaaggg cgggaaaagc gcgatggcag tacgctgggc
     5341 gatcgcctga attactggga tgtgaaaatg agtattcagt atgactttat gctgaagtaa
     5401 gaaataacgc cggagagaaa aacctccggc gtttcagatt gttgacaaag tgcgcgtttt
     5461 ttatgccgga tgcggcgtaa acgccttatc cagcctacaa aaactcataa attcaaagtg
     5521 ttgcaggaaa aggtaggcct gataagcgta gcgcatcagg caatctctgg tttgttttca
     5581 gatgaaaacg ccggagtgaa aattctccgg cgttttggcc gtgaattact gctgcggaat
     5641 tgccggtaca gccggaacgt taagagctgg catcgcaaac atgccaacaa aatcttctaa
     5701 cgacattttc tgcccattta acgttatctg accgttagca tattgcaggc tggtggtgat
     5761 ggtattgtcc tgcaaggtgg tcagacggaa catctgcccc attgctgatg caccttcaac
     5821 ttgctgtttc gccagttttt tcgcttgatc ttcctgataa ccttccagct tcgctacctg
     5881 agtcatcaac tcagttgcca tatccaccgg aatggtcagt ttcgcatcca gagatttaac
     5941 cgaacgatct acttcctgcg ccagcgtttg cggcgcttct ttagtcgttg ccggatcttt
     6001 caggaacagc gacagattca gcgcactttc accctgactg tttttccagc ttagcggcgc
     6061 gatagtaatc accggatcgc ctttcaacat cagcggcagg gcgctaaaga aggcttccgt
     6121 cactttctcc tgataaagtt cgggattgtt ggcaatttct ggctgtgcca gcagcgcctg
     6181 agtttgcgcg ttatattgct ggctaaactg atgccaggct tcaccatcaa tctggccgac
     6241 ttttaaagtc agcttgccgc tgcccagatc ctgattctgt accttcaggc tgtttagcga
     6301 gtaatccagt tggctattga tcgttttacc gtcattgacc agatccgatt taccgctgat
     6361 ctccatgcct tccagcagtg ccagttcttt gccttccact gaaatggtca ttttttccag
     6421 tgacagtttt tggtttccta cgcgctcacc aaaacttccc agcgtgctgg aaccgtcggt
     6481 tttcagatta ttaaaggtca actgcacttt ctggttgtat tcgttaactg cgtctatccg
     6541 accactttgc gcctctccgg aaagggagat ggctttgccg tctctgtcag catttaactg
     6601 gaactcgccg ccgctaaagg cgactttttc atccttttgc tcgtaattca gtggattgag
     6661 cgaaatatcg gaactggaat caccgctgta accaatgcgc gagttaatct caaaaggcgt
     6721 ttcacctttt gccatatcaa acagtggttt gcttacttcg ttattaacca gcgtggtttg
     6781 aattgatgcc atcgacggga tcaggttcag ttttttaagc tgggcaagcg ggaagggacc
     6841 atgatcaacc gattcgttga agatgacgct ctgaccgctt ttaatccacg gattttcttt
     6901 cccggcaatg ggtttcacca acagttgcaa ctggctgctg aatacgccgc gatgatagtt
     6961 ttgataactc acttccaggt tggattcagg agctgtcagt ttgagttgcg cgttcgcctg
     7021 cgcgaccatg tcttcgagat gggtttcaat cttcttgcct gtataccatg cgccgcctgt
     7081 ccagactacg cctagcgcaa caatgacgcc taccgctacc agcgatttat tcataatgat
     7141 tatccataaa atgaaatcag gcggactggc cgcctgaagg tgttataagc ctttaataag
     7201 cttagcaaga gatgttaatt ttttcagtaa gctcttacag cttgttgtaa acacgcgcta
     7261 aacggccgtg gcctttgaca gtcaccggtg attcgttggc ggcaataaac gctgattcac
     7321 ccggtttaag ctgtaactgc tgagaacctt tccacaacgt tgcatcgcct tcgacgcaga
     7381 acaaaatggc ggcactctgc tggctgatgg tggtttcttt atcactaagg tcatgcagcg
     7441 agaaggcaaa atcatccaca ggaatcggga agtccagttc tgcaccttgt ttcaccggct
     7501 gggtcaacaa ctggttagcc ggtttggctt cgaatttcac attggcaacc agttccggaa
     7561 tatcaatgta tttaggcgtc agacctgcac gcagcacgtt atcggagttc gccatcactt
     7621 ccagcgccac gccttgcagg taagcgtgcg gtgtttcagc gaacaggaac atcgcttcgc
     7681 caggattcaa tttcaccaca ttcagtaaca gcggggagaa caggccgctg tcttccgggt
     7741 aaaattcaga aattaaacga atcgtttgcc acggttcacc ctgctgactg tcgagcgccg
     7801 attttaaaat cgccagcgcg cgggattttt cttcaccctg catattcaac aggttggcga
     7861 acagttcgct taaacgttcg gcatgtggct gttgtaaaaa gtgagcaatc gccggatgtg
     7921 caccggcgac cggctggagt agggagacaa tctcggaaaa ttcacgaaac gcgttcatcg
     7981 caaggaaagg cgtcagcgca aaaaccagct ccggcttgtg gttaggatct ttatagttac
     8041 gctcggcggc atccatcggg atacctgcgg cattttcttt ggcaaaaccg atttcagaat
     8101 tgtgtttgtt tggatgaacc tgaatggaga gtggctgtgc tgcgcataat actttgaaca
     8161 ggaaaggcag ttcgccaaag cgtttggcaa cggcctctcc gagcagagtc gatttatcac
     8221 tctcaatcac atcacgcagt gaaacgatat ctccggcggc attctgcact cgtgaactgc
     8281 ttttcggatg tgcgcccatc cacagctcgg ccatcggctg gctggacgga ttttccatac
     8341 cataaagttc agtcaacgcc gttttgctac cccaggcata gttttgcact gagttaatga
     8401 gtttttgcat gatcaatccc tgttttaatg tggaaattaa tcccgctatt aaagcaagaa
     8461 tcctacggga agtaacctgg agccgcaaaa agtcgtacta gtctcagttt ttgttaaaaa
     8521 actgtgtagg atattgttac tcgcttttaa cagggcaacg gaacacccgc ccagagcata
     8581 accaaaccag gcagtaagtg agagaacaat gtcaaacaaa ccctttcatt atcagtctcc
     8641 ttttccactc aaaaaaaatg atactgagta ttacctgcta accagtgaac acgttagcgt
     8701 atctgaattt gaagggcagg agattttgaa agtcgcaccc gaagcgttaa ctctgttggc
     8761 gcgtcaggcg tttcatgatg cgtcgtttat gctgcgtccg gctcaccaac aacaggtggc
     8821 cgacattctg cgtgacccgg aggccagcga aaatgataaa tatgtggcgc tgcaattcct
     8881 gcgtaactcc gacatcgcgg cgaaaggcgt tctgccaacc tgtcaggata ccggcaccgc
     8941 gattattgtt ggtaaaaaag ggcagcgtgt atggaccggt ggtggtgatg aagcggcgct
     9001 ggcgcgcggt gtctataaca cttatatcga agataatctg cgctactcgc aaaacgcgcc
     9061 gctggatatg tataaagaag tgaataccgg caccaatctg ccagcgcaga tcgatcttta
     9121 tgccgttgat ggcgacgaat acaaattcct ctgtatcgca aaaggtggtg gttcggcaaa
     9181 caagacgtat ctctatcagg aaaccaaagc gttactgacg ccggggaaac tgaaaaatta
     9241 cctggttgag aagatgcgca cgctgggtac cgcggcctgt cctccgtatc atattgcgtt
     9301 cgttattggt ggaacttctg cagaaacgaa ccttaaaacg gtgaaactgg cttccgcgaa
     9361 atactatgat gaactgccaa cggaagggaa tgagcacggt caggcgttcc gcgatgtgga
     9421 actggaaaaa gaattactga tcgaagcgca aaatctcggt ctgggtgcgc agtttggtgg
     9481 taaatacttc gctcacgaca tccgcgtgat tcgcctgcca cgtcacggcg catcctgtcc
     9541 ggttggtatg ggtgtttcct gctctgctga ccgtaatatc aaagcgaaga tcaaccgtca
     9601 ggggatctgg atcgaaaaac tggaacataa tccaggtaaa tatatccctg aagagctgcg
     9661 taaagcggga gaaggcgaag cggtgcgcgt tgaccttaac cgtccgatga aagagatcct
     9721 cgcacagttg tcgcagtatc ccgtttctac acgcttatcg cttaacggca cgattatcgt
     9781 tggtcgtgat attgctcacg ccaaactgaa agagcggatg gataacggtg aagggctgcc
     9841 gcaatacatt aaagatcatc cgatttacta cgcgggtccg gccaaaacgc cggaaggtta
     9901 tgcctccggt tctcttggcc caacgaccgc cggacggatg gattcttatg tcgatcaact
     9961 gcaagcgcag ggcggaagta tgatcatgct ggcgaaaggc aaccgcagcc agcaggtgac
    10021 ggatgcctgt aaaaaacacg gcggcttcta ccttggcagt atcggtggtc cggccgctgt
    10081 attggcgcag ggaagtatta agagcctgga atgtgttgaa tatccggaac tgggaatgga
    10141 agccatctgg aaaattgaag tggaagattt cccggcgttt atccttgtgg atgataaagg
    10201 aaatgacttc ttccagcaga tacaactcac acagtgcact cgctgtgtga aataaacaga
    10261 gccgcccttc ggggcggttt ttttacatgg cacgaaagac caaacatttg ttatcaaata
    10321 gtaaataatg agtgagctaa aagttgctta acgaaagcaa aacagaaaga aaaaattaat
    10381 caggtgagga gcaggccatg aatacagtac gcagcgaaaa agattcgatg ggggcgattg
    10441 atgtcccggc agataagctg tggggcgcac aaactcaacg ctcgctggag catttccgca
    10501 tttcgacgga gaaaatgccc acctcactga ttcatgcgct ggcgctaacc aagcgcgcag
    10561 cggcaaaagt taatgaagat ttagacttgt tgtctgaaga gaaagcgagc gccattcggc
    10621 aggcggcgga tgaagtactg gcaggacagc atgacgacga attcccgctg gctatctggc
    10681 agaccggctc cggcacgcaa agtaacatga acatgaacga agtgctggct aaccgggcca
    10741 gtgaattact cggcggtgtg cgcgggatgg aacgtaaagt tcaccctaac gacgacgtga
    10801 acaaaagcca aagttccaac gatgtctttc cgacggcgat gcacgttgcg gcgctgctgg
    10861 cactgcgcaa gcaactcatt ccgcagctta aaaccctgac acagacgctg agtgaaaaat
    10921 cgcgtgcatt tgccgatatc gtcaaaatcg gtcgaaccca cttgcaggac gccacgccgc
    10981 taacactagg gcaggagatt tccggctggg tagcgatgct cgagcataat ctcaaacata
    11041 tcgaatacag cctgcctcac gtagcggaac tggctcttgg cggtacagcg gtgggtactg
    11101 gactaaatac ccatccggag tatgcgcgtc gcgtagcaga tgaactggca gtcattacct
    11161 gtgcaccgtt tgttaccgcg ccgaacaaat ttgaagcgct tgcgacctgt gatgccctgg
    11221 ttcaggcgca cggcgcgttg aaagggttgg ctgcgtcact gatgaaaatc gccaatgatg
    11281 tccgctggct ggcctctggc ccgcgctgcg gaattggtga aatctcaatc ccggaaaatg
    11341 agccgggcag ctcaatcatg ccggggaaag tgaacccaac ccagtgtgaa gcattaacca
    11401 tgctctgctg tcaggtaatg gggaacgacg tggcgatcaa catgggtggc gcttccggta
    11461 actttgaact gaacgtcttc cgtccgatgg tgatccataa tttcctgcaa tcggtgcgct
    11521 tgctggcaga tggcatggaa agttttaaca aacactgcgc agtgggtatt gaaccgaatc
    11581 gtgagcgaat caatcaatta ctcaatgaat cgctgatgct ggtgactgcg cttaacaccc
    11641 acattggtta tgacaaagcc gcggagatcg ccaaaaaagc gcataaagaa gggctgacct
    11701 taaaagctgc ggcccttgcg ctggggtatc ttagcgaagc cgagtttgac agctgggtac
    11761 ggccagaaca gatggtcggc agtatgaaag ccgggcgtta atctgcaaca tacaggtgca
    11821 gccgtggaat gatcaaacga agcggctgcg cctgaggttt ataacggtgc tgcacattgt
    11881 cggcatcgta atttaacaac tcaccaacgt ccggcacgcc cgcgccatta tcccgattaa
    11941 tcagtgcaat cagtggtgta gggcaggcgt gttggacttg tttttgatct cctttgtacc
    12001 agacgcgggc aatcggctgc accttcactg gacgtttgat ttttaacttc gcgttctgtg
    12061 gcagggcagc gatatcctga tactctcgct ccagttttct ttgccactcc tcgcgcgtcc
    12121 acggtgcgac actgcgtggt gatttcaggc ttttttccag ctgtgccagg acttcatcac
    12181 gatgcaaatt cttaatgata tgtttattag cccaaccaaa gcgtaaagtg gcggggtcgt
    12241 gcagaacggt gagcgtgcgg taagcattaa gggtgatcag ccccggcaaa tgacgatgca
    12301 cccattcaaa acgtgccgcg gtggggagtt ctgattcaac cgtgacgata tgctcgaacg
    12361 tggtcttgag tttattgatg tgctgaatat gactgaccaa cgctgcttgc gaaaggttat
    12421 cgacctggta acacaacacg ccaggcagac ggacagcggc cttgctgctg cgattttcgg
    12481 actgttgttg aataaataaa tggcggaaat gacgcaacgc cagcgactgc gcgtcgttgc
    12541 cgagatgttg ttttacctca atacgattaa gcggattatg ctcatcctct ttttttacct
    12601 ccggcaaaga gaacacgcgg gcaaccaata gcttgtgttg ctcaagatga gcggcaaatg
    12661 tagccagctc ttgttccatc tggcgaaagg tagtgttgag tcggtctacg agatcgtaac
    12721 gcgccataat attaaccact ttagttacaa catacttatt ttatagcaca gtcgtggtga
    12781 cttgaccatc gtgcgcgtag tgtcaggcag aggtaaattg cgggatgtta tgccataacg
    12841 gccagctaaa cgagaagcga gcaccaccca gttcgctgat gtcacaatta accgtaccgc
    12901 ccattgtcag tgctattgag tggacaattg ccagccccag cccgcagccg ccggttgagc
    12961 gatcccggct gggatcgagg cgaacaaaag gttcaaagat atgttcgcgg ttttctgggg
    13021 caatccctgg gccatcatcc tcaacaatta atgtcgctct attccccgac agtagcaggc
    13081 tggtttcaac cgttgaatgg cagtagcgca gggcgttatt gagcaaatta tccagcacgc
    13141 gctccattaa gcgcatatcc aacgccgcat aatggccttg catgagcgtt tttatccgta
    13201 ccgttttatc gggcgttact gcctgaatat ctgccagatg cgttgacagc cacaacggca
    13261 ggtctggttc gctaagatga agctcgtttt gtgggcgatc gagtcgggca taagtcagca
    13321 gctcttcaat taaagcttca agttgactga tatcacgatt caacgcctgg gattcggcgg
    13381 cgctcaggtt atcgctcatc tccagtcgat aacgcaggcg cactaacggt gttcgcagtt
    13441 cgtgagcgat accgtcaata agctgttttt tgctggcaat taaggcgttg atattgtccg
    13501 ccatctggtt aaatgcgata ccaagtcgtt caaagctcga accctcatca aagtggatac
    13561 gttcattgag atgcccatcg ccaaatcgtt gcgccgctgc ttccagtttt aacatatcct
    13621 gccagtgcgg acgcatccag ataaacaccg gaaaggcgag ggaaatagca ataaaagcga
    13681 tcagggcgat atccagcaat cgcatttgat ggaggtaata aagataagga acaggaccaa
    13741 ctgccagcac gtagtggctg cgcgggatac gctgcaaaaa cgtgtactga tcgtccaggg
    13801 cgacaatttc gccgccacgc agtcggtgca tggaaatatc atcaagatgg tatttactca
    13861 gtggctcgac acgcagatcg aaagagagat ttaaatccat ctctttcagc gtcttacccc
    13921 agtcgtgtgg ggggatctct cgcaattcgc tgcgcatcag atacagcgaa ctgttcatca
    13981 aatcatccag cgactgtttg cccgcgcgtt cggcggtaaa tttgtacacc agcccaacca
    14041 gcagagacat cacaaggaag cagacaaaca ataacaggta aaactggata aacaattttt
    14101 tcattggcat cgcccgcaac agaatactat tcgtattaat tcacgactgt ggagcaatgt
    14161 aaaccgccaa ctggataaga ccttgtttgg aagataatgt ccggaacaga cggatgtatc
    14221 cccggtgtat tcatattcag taaattcaat tttccaccgg ggcttgttta ttatgctaag
    14281 gtttaattta acgtgacgct ttgttgttct ttagtctctc tatccttaag aagacgggct
    14341 gatattacac cggacattat tgaaccattc acattaagcg ctgttcttgc catatcaacg
    14401 atcggctcta tggcaataaa aataactact aatgcgacag ggaaattaag tgttgaaagg
    14461 acaatcagcg ccgcgaatgt cccaccacta cctacgcctg ctacccctat tgagccaagc
    14521 gcgatggcag gtaaaagagt tgcgaggaat tgcaatgaga agggatcgat acccattgtt
    14581 ggagcaatca tcgccaccat cacggcagga taaatcccag cgcagccatt ttgtcccatg
    14641 ctggaaccaa aagacgctgc aatattagca atcgtatttg gaacccccaa tttttccaat
    14701 gcagaaatag ccagaggaat tgaagccgca ctactgcgag aaacaaacgc aaaggtaagt
    14761 acttgccaga cggtcttgta atagttaacg gggtttttac ctattaacag caaaatgccc
    14821 gagtgaacaa taaacatcac aaaaacggcc agataacagg cgacgatgaa cccgagcaaa
    14881 ttggtaaact gtgaaagctg atacgttgag aagactttcg ccattagtgc cattacccca
    14941 taaggcgtca gcgcaatgac aatacggacc attttcatta cccatatttg aatcgcgttg
    15001 atccccgcgc tcaatttttc gccctctgct ggtgcatctt tctttacttt taacagcgca
    15061 ataccagcaa gaattgtaaa gatgacgatg ccaataaccg aaacacttct tgccccggtc
    15121 agatcaagga agaggttggt tgggatcaat gacgtaatga gtcgcggtaa agataccgca
    15181 gctgttttcg agacgtcatc gctggttaaa gaggattgca tatgaccaaa ggcgctggca
    15241 tcaaggccaa ttgcactggc aatagccagg ccaactaaac ctgcaatcat gactaaacac
    15301 aacatacagg caacaatagt taaagacatc ttgccaatac cagcgctatt ttcgagctta
    15361 ttaatcgctg aaagaatcga aatgaatatg agtggaacgg caacaagttt taataaatta
    15421 acataaccgt taccagcaat attataccat tcgataacgg tttcactgct tgtctcagga
    15481 atgaaatgca gggagatacc aatgatagcc cccaggaaaa tcgcgataaa aacacttttg
    15541 gaaagggatt cgctcttttg atccgttttg aaaatccccc atgagcagat caaaaaaagc
    15601 acaatatatg tgaaagttgt aaccgttttt taagcctctt gcctcattca gcacctgaaa
    15661 tgccggaaag agtttgttgt aaaaaatctg cgccataaga cgcagggacg aggcgattat
    15721 agagaaattt tttctcattt tcatatcaaa tgtagggggt ttggaatttg aataagctct
    15781 tattaattga attcgttaat tgttctcttt ttcagatgaa caaatattag tcatctgatt
    15841 gtttattgtc actatcccat gcatgaggcg caaaaagata gcctttgtta cgcacagttt
    15901 taatgcgata aggttctgcg gcgttatcga gcagtttttt tcttaaccgc gaaatagcca
    15961 cgtccacgct acgatccagt ccgtcataac tgacgccgcg taaatttttc agcaatgcat
    16021 cgcggtccat gatttgcccg gcatgcgtag ctagttccca caataattcg aaatcagctg
    16081 tcgagagcga gatttcagtg ttagccaggg tgactacgcg gttgatgggg tcgatagtta
    16141 acgtgccgaa atgcagggct ttgtagggag taagagacgt ttcctgaaga cctttggtca
    16201 gtgtggcttg ctcattctga cgcaaatgca aacgtaaacg cgctagcaaa acagcagggg
    16261 gcgtcgtttt gagaatatag tcgcaggcac ccatttccag tgccaggatg tggttcatat
    16321 cgctatcgag agaggttaga agaacaatcg gtccagacca ctttgcgcgt aaatcacgac
    16381 aaatggtcat gccgtccttg cctggtagca tgatgtcgag taacaccaaa tccggatttt
    16441 ctcgcaaaat ggtttcttcg gcctggtcgc cgcgcggttc tacggtaacc tgcatatcat
    16501 gttttgccag gtacgcggca atcagtgaac cgacttccgc atcatcttcc acaaatacga
    16561 tagtgttcat aacattcacg gtagatataa aaacgtcaca atacaccgcg ctgtttttac
    16621 ttactatcaa tcgtttctaa acaagttttc atcaggtatt ctactgatga cttgttatgg
    16681 aagtgttaag gtaaaaagat ggggctggta atcaaagccg cgctcggtgc gctggtagtg
    16741 ctgttgattg gtgttttagc aaaaacgaaa aattattata tcgccgggct gattccactt
    16801 ttcccgacct ttgcgcttat cgcgcattat attgttgcca gcgaacgcgg cattgaagcc
    16861 ttacgcgcaa ccatcatttt tagtatgtgg tcgattattc cctattttgt ttacctggtg
    16921 tcgctgtggt atttcaccgg aatgatgcgc ttgcccgccg cttttgttgg ttctgttgcg
    16981 tgttggggga taagcgcatg ggtgttgatt atatgctgga taaagctgca ttaacgcaga
    17041 tgacgaccgc catcaagtgg gaaactgcgt ccggtgacaa agcaactggt aagtaagtaa
    17101 tcgaccaggt cgatcacttc tttctcgcca ggcgcggttt tcatcagtga tttattcagc
    17161 gcctgttgtc gatattcggc atcatcatgt tcattaaaca ggatcagcga tggcgcaata
    17221 gaattcactt tcacttccgg tgccagcttg cgggcaaacg agcgggtcat attatccagt
    17281 gccgctttgc ttgcagcata cgcaacatgt ttgtcgctac cgcgctccac cacataatcg
    17341 gtaaagtgaa tgatatcgct ggcggcgtgt ccgtgcccac gcagtaatct ttccagcgca
    17401 tggttgagca ggtatggggt attaacgtgg atctgcatca tgcaagccag tacgtcggcc
    17461 agtggcgcac ccggtttttc cgcaatccac gcactggcgt tatgcaaaat agcacgcaga
    17521 ccatgggtgg tttttagtac ttcatcggca aacgccatca caccgtcgtt ggtcgaaaaa
    17581 tcagcctgaa tacactgcgc acctgcatta atcagtccat caatggctgg atagtgtgtc
    17641 cgatagctga caatcaccgg ttgcttttga ttaatgaaat gccatgcgag ggcgaggccg
    17701 atgcgacgac ctccgccagt aattaatatt ggcaagggct gggttttacc catcgttatc
    17761 tcctttgcta tccaacgatg ggatgaacgt tatcccacca gcatccaggt cgccggaact
    17821 gaggcaatta acagcatgcc aatcagcacc atttcctgac ggttcagtac attatcatgt
    17881 gtatgcgttt tgcgcgcata caggaaaacc agcagccccg gtgcatacag aacaacggat
    17941 aacagcaagt gcattggacc agaggcgtac aataaccata agccataaat gcaggcaccg
    18001 acacctaccg ctttatgcag tggacgggtt gcgattttca gcaagaacgc gccgaccagg
    18061 aaatagggca ccagaatcat ttctgaggcg atggtcagca acgtgttgta atcggaaccg
    18121 gtcagccaga tgagtaccag acaaatttgc acgcagatgt tggtcaacca cagtgaggcg
    18181 gatggcgcag cttgtgcgtt ctggcgcgca aaaatgcgtg ggaatgcttt atgagtggca
    18241 gccaggaacg gaacttctgc cgccatgatg gtccagctta agtacgcgcc gcaaacggaa
    18301 acgatcaaac cggcagcgat gatgatttcg ccccatggtc ccatcatttc caccatcaga
    18361 ccggccattg acgggttacg aatttcagcc agttcaggac gtgccaccac acccagcgaa
    18421 agcagcgtta ccagcaagta aacgcccaga gcggagagaa ccgccagcag tgtcgctttg
    18481 ccaacatcac gtttattacg cgcacgcgca gaaacaacca cggcaccttc cacaccaatg
    18541 aaaacccaca gggtgatcag catggtgttt ttcacctgtt cccaaacagg tacgccaagt
    18601 gcaagtccgg tgaagtcgag cttgaaggta tccagtttga acatcatcat cgccagcaca
    18661 acaaacagac ccagcggcaa caattttgcc agtgtcgcca ccaggttaat gctggcagcg
    18721 gtttgcaccc cgcgcaggat caaaaagtga acaatccaca ataaagccga tgcaccgact
    18781 atcgattgcc aggtattacc atcgccaaac aggcgcaatt ccggcgtgtc cgtaaaaaag
    18841 cttaacgcgg aaaaaacgat caccagatag gagacgttgg cgatgaccgc gcacagccag
    18901 tatccccatg cggaacaaaa gccgattagc tcgccaaacc cttcgcgggc ataggtaaag
    18961 ataccgccat caagttcggg acgaatgcgc gtgaggatca gcatggcaaa ggccagcaat
    19021 aaaatgccag cgccagtaat accccagccg atgagcagtg ctgccgggct ggcaactgcc
    19081 gccatatttt gcggcagact gaaaacaccc gcgcccagca ttgagcttaa taccagcgcg
    19141 gtgagtgcgc tcagtcccag tttcttttcc atcgatatcc agtgattaaa acgtgataag
    19201 aagaataaat attagggcaa aacgcataaa aatggagttt gccactgaag cgcgggattt
    19261 tacgaagaga tgtgtctgca tgcaatggtc gcggggaaat ttgtgactaa aaatgcgaaa
    19321 ggcggcatca agccgcctta taggagtaac tgaatagtta tttatacaga tctgcgctga
    19381 cggtcaggtt attaccacgt ttctgccact ggcgggtgat gtggtaatac ttagcacctt
    19441 tcttcgcggc acgtttcgca acctgatagg agacttcggt catgttgccg tagttgccag
    19501 agaatttgat gctgtcgaac gggaccatca tcgctgctgt cgctttgttc agttcttcga
    19561 ctttagtgcc atccgggagc gtgacggtgt aacgcccgcc ttttgatgac tgggtttcaa
    19621 agaagcgacc gacttcagaa cttggtgatg cggtagtcgc aacacccggg atctcaactt
    19681 tcttcgcggc ttcgccaccg gcagccagag ctgcacgtcc tgcttcggaa tctgccggga
    19741 tcacatccgg gctctggacg atacgtttct tagcatcttt tttatagatg aatgcagtaa
    19801 tacgctggtt gccgccctgg ttggcatcga tttgacgaac gatgtagaaa gagtaggcac
    19861 ctttcgcttt tgccgctttg gtgatggcat cattgacttc tggctggcta cgatagaagc
    19921 cctggacggt gaccgtgtca aacggttcaa tcagaacagc ctgatctttc ggcagttcga
    19981 caacaccgtt aattacgcga ttacttgttt cttctgcttt ttcagcatcg gctttataga
    20041 ggtcagcgac cacacgccag ttaccgctgt taccaaaatc agaagtgtcg acaacataaa
    20101 aagaggcggc accttcttta tctgcgcgac gagaaacggc tttcaccgct tcgccaatag
    20161 cattaaaacg accggtaacc actacacggt caaaaggttt aaccgctgcc gcttgctccg
    20221 gtgtcagttc tgttgctgcg ttaacggaga atgccgtagc agaaagcagt gccgacgcca
    20281 ggagggtgtt cttaagcttc ataaaaataa tccttcgcct tgcgcaaacc aggtactggt
    20341 attgttatta acgagaaacg tggctgatta ttgcatttaa acggtgtaac tgtctgcgtc
    20401 atttttcata tcacattcct taaaccaatt ttaatcctgc tcaaatgacc gtctatgctt
    20461 aaaaaacagc cgtatcagca tcattactac tgaagcaact gaattgtata agttaattta
    20521 atgttaagta gtgattcgtg ccggggcgat gtctcgtttt acccgaccgt cgaagacaat
    20581 tatcagtctt tatccggcgt tctaaggtgt ttatcccact atcacggctg aatcgttaat
    20641 attttgcgag ttcacgccga aatactgatt tttggcgcta gatcacaggc ataattttca
    20701 gtacgttata gggcgtttgt tactaattta ttttaacgga gtaacattta gctcgtacat
    20761 gagcagcttg tgtggctcct gacacaggca aaccatcatc aataaaaccg atggaaggga
    20821 atatcatgcg aattggcata ccaagagaac ggttaaccaa tgaaacccgt gttgcagcaa
    20881 cgccaaaaac agtggaacag ctgctgaaac tgggttttac cgtcgcggta gagagcggcg
    20941 cgggtcaact ggcaagtttt gacgataaag cgtttgtgca agcgggcgct gaaattgtag
    21001 aagggaatag cgtctggcag tcagagatca ttctgaaggt caatgcgccg ttagatgatg
    21061 aaattgcgtt actgaatccc gggacaacgc tggtgagttt tatctggcct gcgcagaatc
    21121 cggaattaat gcaaaaactt gcggaacgta acgtgaccgt gatggcgatg gactctgtgc
    21181 cgcgtatctc acgcgcacaa tcgctggacg cactaagctc gatggcgaat atcgccggtt
    21241 atcgcgccat tgttgaagcg gcacatgaat ttgggcgctt ctttaccggg caaatcactg
    21301 cggccgggaa agtgccaccg gcaaaagtga tggtgattgg tgccggtgtt gcaggtttgg
    21361 ccgccattgg cgcagcaaac agtctcggcg cgattgtgcg tgcattcgac acccgcccgg
    21421 aagtgaaaga acaagttcaa agtatgggcg cggaattcct cgagctggat tttaaagagg
    21481 aagcgggcag cggcgatggc tatgccaaag tgatgtcgga cgcgttcatc aaagcggaaa
    21541 tggaactctt tgccgcccag gcaaaagagg tcgatatcat tgtcaccacc gcgcttattc
    21601 caggcaaacc agcgccgaag ctaattaccc gtgaaatggt tgactccatg aaggcgggca
    21661 gtgtgattgt cgacctggca gcccaaaacg gtggcaactg tgaatacatc gtgccgggtg
    21721 aaatcttcag tacggaaaat ggtgtcaaag tgattggtta taccgatctt ccgggccgtc
    21781 tgccgacgca atcctcacag ctttacggta ctaacctcgt taatctgctg aaactgttgt
    21841 gcaaagagaa agacggcaat atcactgttg attttgatga tgtggtgatt cgcggcgtga
    21901 ccgtgatccg tgcgggcgaa attacctggc cggcaccgcc gattcaggta tcagctcagc
    21961 cgcaggcggc acaaaaagcg gcaccggaag tgaaaactga ggaaaaatgt gcctgctcac
    22021 cgtggcgtaa atacgcgttg atggcgctgg caatcatcct gttcggttgg ctgtcaagcg
    22081 ttgcgccgaa agagtttctt gggcacttca ccgttttcgc gctggcctgc gttgtcggtt
    22141 attacgtggt gtggaatgta tcgcacgcgc tgcatacacc gttgatgtcg gttaccaacg
    22201 cgatttcagg gattattgtt gtcggagcac tgttgcagat tggccaggga ggctgggtta
    22261 gcttccttag ttttatcgcg gtgcttatag ccagcattaa tattttcggt ggcttcaccg
    22321 tgactcagcg catgctgaaa atgttccgca aaaattaagg ggtaacatat gtctggagga
    22381 ttagttacag ctgcatacat tgttgccgcg atcctgttta tcttcagtct ggccggtctt
    22441 tcgaaacatg aaacgtctcg ccagggtaac aacttcggta tcgccgggat ggcgattgcg
    22501 ttaatcgcaa ccatttttgg accggatacg ggtaatgttg gctggatctt gctggcgatg
    22561 gtcattggtg gggcaattgg tatccggctg gcgaagaaag ttgaaatgac cgaaatgcca
    22621 gaactggtgg cgatcctgca cagcttcgtg ggtctggcgg cagtgctggt tggctttaac
    22681 agttatctgc atcatgacgc gggaatggca ccgattctgg tcaatattca cctgacggaa
    22741 gtgttcctcg gtatcttcat cggggcggta acgttcacgg gctcggtggt ggcgttcggc
    22801 aaactgtgtg gcaagatttc gtctaaaccg ttgatgctgc caaaccgtca caaaatgaac
    22861 ctggcggctc tggtcgtttc cttcttgctg ctgattgtat ttgttcgcac ggacagcgtc
    22921 ggcctgcaag tgctggcatt gctgataatg accgccattg cgctggtatt cggctggcat
    22981 ttagtcgcct ccatcggtgg tgcagatatg ccggtggtgg tgtcgatgct gaactcgtac
    23041 tccggctggg cggctgcggc tgcgggcttt atgctcagca acgacctgct gattgtgacc
    23101 ggtgcgctgg tcggttcttc gggggctatc ctttcttaca ttatgtgtaa ggcgatgaac
    23161 cgttccttta tcagcgttat tgcgggtggt ttcggcaccg acggctcttc tactggcgat
    23221 gatcaggaag tgggtgagca ccgcgaaatc accgcagaag agacagcgga actgctgaaa
    23281 aactcccatt cagtgatcat tactccgggg tacggcatgg cagtcgcgca ggcgcaatat
    23341 cctgtcgctg aaattaccga gaaactccgc gctcgtggta ttaatgtgcg tttcggtatc
    23401 cacccggttg cggggcgttt gcctggacat atgaacgtat tgctggctga agcaaaagta
    23461 ccgtatgaca tcgtgctgga aatggacgag atcaacgatg actttgctga taccgatacc
    23521 gtactggtga ttggtgctaa cgatacggtt aacccggcgg cgcaggatga tccgaagagt
    23581 ccgattgctg gtatgcctgt gctggaagtg tggaaagcgc agaacgtgat tgtctttaaa
    23641 cgttcgatga acactggcta tgctggtgtg caaaacccgc tgttcttcaa ggaaaacacc
    23701 cacatgctgt ttggtgacgc caaagccagc gtggatgcaa tcctgaaagc tctgtaaccc
    23761 tgacggcctc tgcggaggcc gtcactcttt attgagatcg cttaacagaa cggcgatgct
    23821 ttgacctccc gctgtttgtt caagcgcaat tttgacaata attgtcaacg gcacggaaag
    23881 cagcataccc accggtccta acaaccatcc ccaaaaaatc aacgacaaaa ataccaccaa
    23941 tgtcgaaagc cccagcccac gtcccatgat gcgcggctcc agaatattgc cgaagaccag
    24001 attaatcagc agatatcccg ccagcaccag caacgcttcg tagaagccat taaacaccag
    24061 tacctgagcg atagggggga ttgccgcgag aactgaacca atattaggga tgtaattaag
    24121 cgcaaaggcc agcaatcccc agacaaaagc gaagcgaaca tcgagtgcgg cgagcatcgc
    24181 ccaggcgacc aggccggtga tgatgctgat ggctgttttc agcaccagat aatgagaaac
    24241 actgtcaatc gcacgttgaa tcgccgccat cccttcaacc ggacgcgcca tcatttgctg
    24301 aaattttccg ggcaattgtg gcacttcgag cagcataaac agcaccgtca gcagcaataa
    24361 aaatattgat gacatggcat tagataactg cgtcaataag ttggtgagca acgtcatcgc
    24421 cgcgttcgga tcaatgtaat gcgccagttg gtcaactgag acgtcaatcc ctacgcgttg
    24481 caacaacggc tcaagagctt gcagcggcgt cataatagag ttgcgatatt gcggtaacgt
    24541 ccgcgtcaac tcgttgagcg cggaacccag ataagctaat agcaacacca tcgccatcac
    24601 gatgattgtc atcaaaatcg acactgccag tacacgcggc acacgccagc ggaccatatg
    24661 ttgcaccagc gggttaagaa taacagcaat aaataatgcg agaataaacg gcacgatgat
    24721 ctcggcggca aaacggatac cgcagagaat aatgaccagc attcccaaca taatgacgat
    24781 ttttaggcca ttgagcgtga tgatcggctt tgccatgctt gtccccgttt ttcttccttt
    24841 gctaataata atgtcttttt cgctccgtca tcataacgaa aatcaaaccg ctcaggtaaa
    24901 gaagtgaaaa tattttgagt taattcttaa gctatgatac aaatcaggcg tgttcaacta
    24961 ccgaggacaa ttatcatctg cgatgacgag aagcaacact gcggataatt gtaatattat
    25021 ggacaatttg ttcaggacgt tattttctac atttacccat cttcgtacct catccactat
    25081 cttgctcgta ggtgagcagc actggcgcaa cgcgctttag tcctttcttc tgcctaagct
    25141 tgccctatgc gcagggcttg accactttac tttggtttcg ctgaattaag cgaaaattaa
    25201 aatagttctc ttgcaggaga aggacaatgt atatttattg gattttatta ggtctggcta
    25261 ttgctacaga aattaccggt acgctgtcaa tgaaatgggc gagcgtcagt gagggaaatg
    25321 gcggctttat tttaatgctg gtgatgattt ctctgtcgta tatatttctc tctttcgccg
    25381 ttaaaaaaat cgccttaggc gtagcttatg cgctgtggga aggtatcggt attttattta
    25441 ttaccttgtt tagcgttttg ttattcgacg aaagtttatc gctgatgaaa attgccgggt
    25501 taaccaccct ggtcgccggg atagtgttga tcaaatcagg tacccgtaaa gcgcgtaaac
    25561 ctgaactgga ggtgaaccat ggcgcagttt gaatgggttc acgccgcctg gctggcattg
    25621 gcaatcgtgc tggaaatcgt tgctaacgtc tttttgaaat tttctgacgg ctttcgtcgc
    25681 aaaatatttg gcttgctctc cctggcggcg gtgctggcgg cctttagtgc gctttctcag
    25741 gccgttaaag ggatcgactt gtctgtcgtt tatgcattgt ggggcgggtt tggtattgcc
    25801 gccacgttag ccgcaggttg gatcttgttt ggtcaacggt taaatcgtaa aggctggatt
    25861 ggcctggtct tgctgttggc tggaatgatc atggtgaaac ttgcctgatg aagacgctgc
    25921 ccgcgctgtc gggcagcgtt tgaacattat ttttgcgaca gttgatccag cttgtcgcga
    25981 aaaccggtaa cagaaatggc ccggttatcg gcgcgccagc gatctttcgc ggcaggagcc
    26041 gaactttgca ccccaattaa ttgccagccg tcatcggtat gcaacatcag aggcgaaccg
    26101 ctgtcacccg gcaaggtatc gcactgatgt gacatcaccg acgtttgcgc ccagccagtc
    26161 acttcacagt tttgatgact gtataacgta tcgagatgat cttcagggta tcccgcctga
    26221 gtcactttac gaccagccga ttttaatgcg gcagtaagcg cggctttatc tccctcaaat
    26281 aacggcaacg gtgtaatgcc agaaggggga ttacgtagca caatcaatcc gaagtcccac
    26341 ggtgcagctg cgggaggaac aatccaacca tccccatctg cttttaaccg ctttcccagt
    26401 gtcggatcaa cgcggccttc tatgtcgtgg atctcatagc gccaaagacc tttatttgac
    26461 acaaaacgca gcgctaccgc cttatccgct ttacccttag gcggcgtcaa taaacagtgt
    26521 cctgccgtta atgccagatt gggtgcaatc agcgtcgccg tacataaatt gccgctggcc
    26581 gtttccagtt gcccaaccgc atcccacggt gattgggtcg tgtcattcac tggcacacga
    26641 tcatcatgac caaaaaacag ggtgctgacc tcatcgtttg ccgatttggc aacgtctggt
    26701 ttatctgcaa acacaaaagc agacgtcaaa ctaattgcac ccaacactac agcaatggtt
    26761 gtacgcatat cacactctgg tgggtaatta tgattattaa aagcaaaccc tcataaatac
    26821 tatagacggg acgggatgaa agtgggagta aaatcagata actacagtca ggaaagataa
    26881 aaacgggcgg caatcagtgc gcataaaata aggatgataa gaatgaactc aaagcgataa
    26941 cggcccacca tactaccctc cgctaaaggc ggtgctaaac gttacccgct tagcaccgta
    27001 attttcaggc gcgagggggc gcgccagcat tactgttgaa aacttacgct gcgggttgtg
    27061 cagcaggttt tgccggttgc tgatggccgt gtttcccggc gtgtttcttc gctgcctgcg
    27121 ctttttgttc aggggctttc tgttcagctt tcgtattttt atgatgcttt ttagccgcct
    27181 gcgctttctg ggcaggtgct gctttatgct gttttttatg atgtgtagtt ttcgctggcg
    27241 ctgctttggt ggtcgtcgca gtcggagcag gtgtggtcgc agtctctgca gcaaaggcgg
    27301 cagaagacag acccatagca gcggcaacaa ccagagctaa tacttttttc attgtcatac
    27361 cctcaatttg ttttttcatt taaccccact gcggggccgt tgaaataact atatccctgg
    27421 gaatggcaga cttccgtgag tggttggttt cagcgtgtaa cgatatgtac aaacgctgaa
    27481 taaattacag cgttgataat aacgtattgt gacttaagga aaatttagct accaataata
    27541 gtagtcttga tcgggttaac tattatacta cttacaaagc ggtagaataa ctgcgcatca
    27601 gtaataaatg acacacagca aaatgaatcc gtttatttgg gtacttataa tcctgatgac
    27661 gctagatgcg ctgcgggaat tggctggcgc ttcgtctatt ttaggatggc tattaacgtt
    27721 ggtttgagca ggcaataagt ccggacgggt atttaccgca gtccggactt atttttcagg
    27781 cgtgcagacg acgatgcaaa cgcgtcccga ccagcagagc aatgaccagc atcagagcaa
    27841 taaatgcgcc gacgccgttc cagccatagt tatgccagaa aacaccaccc agcgtcccgg
    27901 caatactcga ccccagatag taactgaaca gatacagcga ggaggcctgg cctttagcgc
    27961 gttttgcgcg ggggccgatc cagctgctgg ctactgagtg ggctgcgaag aatccggctg
    28021 agaagagtaa cattccggca aagatcagcc acagcgagct aaataaggtc atcagtaaac
    28081 caaacggcat aactcccgtc gaaaacaaca tcaccggacc gcgcccatag cgggtggtca
    28141 tggttccggc tttgggtgag ctccatgtac cggtcaaata agccagcgat aataagccaa
    28201 ccacggcctg actgacatgc tagggggaaa gcatcaaccg atagccgatg taattaaaca
    28261 gcgtgacgaa cgaccccatc agcaaaaagc cttctgcgaa caataacggt aatccccggt
    28321 cacgccagtg cagacgaaag ttgataaata acgtcttagg gcgcagcgaa gtcgggcaaa
    28381 aatggcgtga ttcagggagg attttccaga acatcaacgc cgaggccagc gcgaaacaac
    28441 cgattgccgc cagagcaatt cgccagttga aaaagtccgt gaagacacca ctaattaagc
    28501 gtccgctcat gccgccaatt gagttgccgc tgatatacaa ccccatcgaa aaggccacga
    28561 aactggggtg gatttcctcg ctaagataag tcatgccaac agctgccacg ccacttaacg
    28621 aaagcccaat caaggcgcgc ataatcaaaa tgccgtgcca gctggtcatc attgtcgaaa
    28681 gtaacgtaca aatggaggcc aacagtagcg ccgtgaccat cactggtttg cgaccaatgg
    28741 catcggatag cgggccagta aatagcaaac cgatagccaa catcgccgtg gaaatggaca
    28801 gtgaaatact actgttcgcg ggcgttaagc caaactcctg cgaaagcacc ggaaggatag
    28861 gctgcacaca atagagaagt gcaaaggttg ccagtccggc agagaacagc gccagggtga
    28921 cgcgcataaa ttgcggcgta ccgcgtttaa taaattgatt tggctgagaa atgctttgct
    28981 tgtcagtgtc gcttgccgga gcgccatcaa cagttgtagt acggctcact tgaaatcctt
    29041 gctaaatatg cctgtggatc aggcttatac atagggtagg aaaatcgaat tgttctgtct
    29101 aatatattaa taatctcaaa taagatgttt taaatatgaa tattgaactt cgtcatctgc
    29161 gttactttgt tgctgttgcg gaagagctgc attttgggcg cgcggctgcc cgcctgaata
    29221 tttcgcaacc gccgctaagt cagcagattc aggcactgga gcaacaaatc ggtgcccgac
    29281 tgctggcacg aactaatcgc agtgtattgc tgacggcagc aggaaaacag tttcttgcag
    29341 atagtcggca aatcctgtct atggtggatg acgctgccgc tcgcgctgaa agactgcatc
    29401 agggtgaagc gggggagttg cgtattggtt ttacttcgtc cgctcctttt attcgggcgg
    29461 tgtccgatac gttatcgctg tttcgccgtg attatcctga tgtccattta caaacccgcg
    29521 aaatgaacac tcgcgagcaa atcgctccgc tcattgaagg aacgctggat atgggattgc
    29581 tgcgtaacac agcgttaccg gagacgcttg aacacgcagt catcgtccat gaaccgctta
    29641 tggcgatgat cccgtacgat catcccctgg caaataatcc gagtgtaaca ctggctgaac
    29701 tggctgaact ggctgaactg gcgaaagaac cctttgtctt ttttgatccg cacgtcggga
    29761 ctgggctgta tgacgatatt ctcggactga tgcgacgtta ccatttgacg cccgtcatca
    29821 ctcaggaggt gggcgaggca atgaccatca tcggtctggt ttccgccggt ctgggtgttt
    29881 caattttgcc tgcgtcattt aaacgtgttc agctcaacga aatgcgctgg gtgccgattg
    29941 ctgaagagga tgcggtttct gaaatgtggt tggtctggcc gaaacatcat gaacaaagtc
    30001 cagctgcgcg taactttcgt attcatctgc tgaatgctct caggtgaggg aaatttcagc
    30061 gaaaaagccc gaaaaatgtg ctgttaatca catgcctaag taaaaatttg acgacacgta
    30121 ttgaagtgct tcaccatagc ctacagatta tttcggagcg cgaaaatata gggagtatgc
    30181 ggtggttgct gaaaaccagc ctgggcacat tgatcaaata aagcagacca acgcgggcgc
    30241 ggtttatcgc ctgattgatc agcttggtcc agtctcgcgt atcgatcttt cccgtctggc
    30301 gcagctggct cctgccagta tcactaaaat tgtccgtgag atgctcgaag cacacctggt
    30361 gcgagagctg gaaatcaaag aagcggggaa ccgtggccgt ccggcggtgg ggctggtggt
    30421 tgaaactgaa gcctggcact atctttctct gcgcattagt cgcggggaga ttttccttgc
    30481 tctgcgcgat ctgagcagca aactggtggt ggaagagtcg caggaactgg cgttaaaaga
    30541 tgacttgcca ttgctggatc gtatcatttc ccatatcgat cagtttttta tccgccacca
    30601 gaaaaaactt gagcgtctaa cttcgattgc cataaccctg ccgggaatta ttgatacgga
    30661 aaatggtatt gtacatcgca tgccgttcta cgaggatgta aaagagatgc cgctcggcga
    30721 ggcgctggag cagcataccg gcgtaccggt ttatattcag catgatatca gcgcatggac
    30781 gatggcagag gccttgtttg gtgcctcacg cggggcgcgc gatgtgattc aggtggttat
    30841 cgatcacaac gtgggggtgg gcgtcattac cgatggtcat ctgctacacg caggcagcag
    30901 tagtctcgtg gaaataggcc acacacaggt cgacccatat ggtaaacgct gttattgcgg
    30961 gaaccacggc tgcctcgaaa ccatcgccag tgtggacagt attcttgagc tggcacagct
    31021 gcgtctcaat caatccatga gctcgatgtt acatgggcaa ccgttaaccg tggactcatt
    31081 gtgtcaggcg gcattgcgcg gcgatctact ggcaaaagac atcattatcg gggtgggcgc
    31141 gcatgtaggg cgcattcttg ccatcatggt gaatttattt aacccacaaa aaatactgat
    31201 tggctcaccg ttaagtaaag cggcagatat cctcttcccg gtcatctcag acagcatccg
    31261 tcagcaggcc cttcctgcgt atagtcagca cattagcgtt gagagtactc agttttctaa
    31321 ccagggcacg atggcaggcg ctgcactggt aaaagacgcg atgtataacg gttctttgtt
    31381 gattcgtctg ttgcagggtt aacatttttt aactgttcta ccaaaatttg cgctatctca
    31441 atttgggcca ggaaagcata acttagactt tcaaggttaa ttattttcct agtttatatt
    31501 tgtgaagcat aacggtggag ttagtgatgc tgaagcgttt ctttattacc ggtacagaca
    31561 cttctgtagg gaaaacggtg gtttcccgcg cattgctaca agcgttagcc tcccagggaa
    31621 aaagggttgc gggatataaa cccgtagcga aggggagcaa agagacaccc gaagggctgc
    31681 gtaataaaga tgccttggtg ttgcagagtg tttcaaccat cgaactgcct tatgaagcag
    31741 ttaatcctat cgcgttaagc gaagaagaaa gtagcgtggc gcacagttgc ccaatcaatt
    31801 acaccctcat ttcaaacggc ctggcaaacc tgaccgataa agtcgatcat gtcgtggtag
    31861 aagggactgg cggctggcgc agtctgatga atgatttgcg tccactctct gaatgggtag
    31921 tgcaggaaca actgccggtg ttgatggttg tcggtattca ggaaggttgc attaaccatg
    31981 cactgctaac agctcaggcg atcgccaacg acgggctgcc gctcattggc tgggtggcta
    32041 accgaatcaa cccaggactg gcgcattatg cggaaatcat tgatgtgctg ggtaaaaaac
    32101 ttccggcacc gctcattggt gaactgcctt atctgccgcg cgccgaacag cgtgaactgg
    32161 ggcaatacat ccgcttagct atgctgcgca gtgtgctggc ggtagataga gtcacggtgt
    32221 aacgtccgcg aaattaccga cgcaattacg caggcaatca ataaaccggg gagtagctga
    32281 tactccccgg tcatttcaca tatcatcaac gtcgacataa tcggcgcgtg cgtggttgcc
    32341 gccaacagtg tcgccatccc ggtcaatccg agtaaaagcg taatttcttc gccatcaggg
    32401 aaccataatc ccaggctacg accatacaac atgccaatgg caagaccgat aaatagcgtc
    32461 ggtgtaaaga caccaccggg tgcgccggaa ccgctactcg ccagcacggc acacagttta
    32521 caaaggaaga tcccggcaat gatcattaac agtggtgggg cggttaaaaa ggattgtacg
    32581 gtgctatagc cgttgcccca cactgcaggt gtgaacaggg aaagcagacc cacgatcaac
    32641 ccgcctagtg ccagttgcca gggcggcgca agtttgagac tcacaaatcc acgatgacag
    32701 gcgttcatta acgttaacaa cagtggtccg cacagacctg ccagcacacc tgtactgata
    32761 atcagcgcat agtcacgagc ctgaaccgtc actgagagtt gtacgttgta gagtaacgcg
    32821 tcgctatgat taatcagatt gctaaccagc catgccacga tggcggaaat aatcaccggg
    32881 ccgagagagg ccaacatcat agtgccaaac agcacttcgg ctataaataa actgccagca
    32941 agcggggcac gataggccgc agccattccc gccgcggccc cacaggcgat ccataatttc
    33001 cattcctggc gtggcgtaaa acgttgggca aaacaggagg cggcaagggc agctaaaaga
    33061 atcatcgcac cttcgcgacc aattgcactg ccgctggtta ctaccagcag cgaggcaagc
    33121 gatttaacca ggcttgctgc gtaatcgaac tgtccatcgg tttgcaacgc ttccatgtaa
    33181 tcggtcggcg catgagggcg ttgttgggta aatttctgcc agcccatcag caacaaaccc
    33241 gccgccagtc cgccgagcgc cggagttagc agccgtcgcc aggaggaaag gtttgtcgct
    33301 gcattgacca gactgccgga gtcattattg aggaacaacc actccagtag cagcatcgca
    33361 tgacgaaacc cggcaacggc aaagacagcg agaataccga cgactgttgc gataagcaga
    33421 cggcggaaca tagtgcgcag atccggatag atgtgtagat gatgcatgag cagaaatggg
    33481 caatgaaaca cgggcgacta ttttgcggca aaagaggcca accgcaaaag cagtgcgtgc
    33541 tttaagagat aaaaaaacgt gacattgtaa cgtattattg ccgaatcgtg cgtaatcgtc
    33601 accatccggc aatattacgg tgatcctatc gaaagagtgg tttaaccgcg acaggaatca
    33661 acagttgtga ttgccattgc gccagcgtta agcgagccag ttcacccagt gcacggtaga
    33721 aagggtgttc tgctttttcg ataaaaacat caagaaaacg tgttgaccac ggaaaaaggt
    33781 gccatgccag caattcttcg cattctgtct ggcgaccatt ctccgccaac cacgcagcca
    33841 tcaacagcag tgaaccaaaa tgatcttccg gttcgttttg cttcatttca aactgaatgc
    33901 ctttctcgcg catccactga cgaagtgcca atgttgaatc gccaaacagc acagattcgc
    33961 gatccagcca gaccgacccc cacggcggag acggcagtgc ccacgggccg acaaacaaac
    34021 gctgccaggc ctgggcgtga gtctcttcac actgtgtctg aaaagccgtc accagcggcg
    34081 ctaatgacgc ttctggtaga ggccactgag tttcccagcc gtcactggtg agtaccgcaa
    34141 ccagaggtgc ggcttccgca ctctctggag cgtaataaaa cagcgcgccc aacacgcgcg
    34201 ccgcgacaga aaaattatct tgctgtgaaa aatgggtcat tcctaacatc ctgaaatgtg
    34261 cgggttgccc cgcaccaatt tgttaacctg caatagccat tcctactgtc atgtgcaggc
    34321 cataaaagag cacacgcccg atcatctcac caacaagtat gaggattagc cccagaacaa
    34381 gtccggcaac gtgaggttca cggcgacgaa tgagcggaca aagccagcat ccaagacctg
    34441 cgcataacaa taccacacgc cagacctgta aggaggcgta atctggtacc agtgcgctgg
    34501 cttgttgcac ggatgagtga atagacgcca gagacaaacc ttgcagcaca atcacctcgg
    34561 cacaagcgat cagcgccaga acgctaatga tggcaaatgg tgtggtatta aatgtaacgc
    34621 gggctgcccg cagaattgcg gcagcaagta ttggaccgct caacaatacc gtcaggaaga
    34681 aagccagcgt agtgtaaccg ttatgccagg ttggcacggt gtcgatttga tacacgcagg
    34741 tcatcatcca gacgaaaatg acgccgagcg ccatgctgac aagtaaccag agtttcccca
    34801 acgcttgcgg cattttaccg atgaccgcca ccagccacca tagtccgcca acggcaaaaa
    34861 atatggaacc ggcagcaatc tcgttactca gaccagaagc tccgattcga ttcagcgagt
    34921 taaacgctcg cagtggcgat cccaggtgca tgacagaggc aataaacccg atgcccatca
    34981 acagccagag gaaaaacatg ccacgaacaa tgtgctgacg gtctgcgtca tttttggcgg
    35041 cgaaccagcc aattccgcta acaatcagcg cgcctactac gcattgaccc aatacggtaa
    35101 agataaccag tggccattca tgccatccat ttcccatttt acacttcctc cggattagcc
    35161 agataaccag tggtatcgcc ggtcgggcgg ctgttggcgt taggtttgat aacgatattg
    35221 ggttttgtga aatgcgcgcg cggcagtggc gcgacggcag ccagagtgcc gtgcttctgg
    35281 cgcagctctt caattggacc gaactcaagt gcacgcagcg ggcaggattc gacgcatatg
    35341 ggttgtttcc cctcggcgac gcgcgaataa caaccatcgc acttcgtcat gtgccctttt
    35401 tcagcattgt actgtggtgc gccgtacgga caagccatgt ggcagtagcg gcagccgata
    35461 caaacatctt catcaaccac cacaaagcca tcttcacgct tatgcattgc gccgctgggg
    35521 cagacttttg tacatgcggg gtcatcgcaa tggttacagg agatggagag ataataagcg
    35581 aacacgttct ggtgccagat gccattatct tcctgccagt cgccgccagc atattcataa
    35641 atacggcgga aactgacttc cgggccaaga tctttaaaat ctttgcacgc cagttcgcag
    35701 gttttacagc cagtacagcg gctggaatcg ataaaaaatc catattgtgt ggtcatgggc
    35761 tactccttaa accttttcga tctggacaag attgctgtgc gacgggtttc cctttgccag
    35821 cggtgaaggg cggtgagagg tcagaatatt gatactgccg ccgtgatcga cccggtcacc
    35881 aaacatatcc gctttaagcc acgcaccttg cccgatggcg gtaacgccag gcagaatacg
    35941 cggagtcact tttgcggcaa tcagcatttc tccattattg ttaaataccc gcacggtatc
    36001 gccatgacgg ataccgcgtg cctgagcatc aatggggttg atccacacct cttgtgggca
    36061 ggcctgctgt aacacatcaa tattgccgta gctggagtgg gtacgcgctt tggtttggcg
    36121 acttcactca gcatccagta gatgggtttg cgttcaaatt ttgctgaggt tgcgggttgg
    36181 gcgaggatca catagcccat attccctgca gattcatgag agataaggtc ttcttgctct
    36241 gttggcatca ggtcaggcaa caggatatcg caatacttag ccgaggccgt catgaagtgg
    36301 tcaatgccaa caatcatctc gcacttgctg tcatcctgaa gcacctcatg ggtgtgattg
    36361 atatcgccat gttgattgat caatgtgtta ctggcgtagc accataaaaa cttgatgggg
    36421 acatccagtt tttcttttcc acgaacacca tcacgggtcg cggtcatttt cgtaccatgg
    36481 tcgatggcat ctgtccatgt aaaaacggaa atctgcgttt taacaggatt ctcgagcatc
    36541 gggaaccatt ctacccccag atcccagcta ccttcgcgta cacctgagtt gccgccgttt
    36601 atgccgacgt taccggtaag aacggaaagc atggcaatag cgcgggacgt ttgttcgccg
    36661 ttggagtgtc gttgtggccc ccaaccctga caaatataag caggttttgc tgaaccgatc
    36721 tctcgtgcca actggataat tttttctgcc gggatgctgg tgatttttgc tgcccattcc
    36781 ggcgttttag ctatgccgtc aggcccttcg cccagaatat aggctttata atgcgcgtta
    36841 cgtggtgcgt tggcgggcag cgttttttca tcgtaaccaa cacaatattt gtcgagaaat
    36901 ggctgatcga ccatgttttc agtaatcagt acccaggcaa tcgcacaggc cagtgcgcca
    36961 tcggtgccag ggcgaatggg cagccattca tcttcacgcc cggcagctgt gtcgttatag
    37021 cgtggatcga tgacgatcat gcgtgcgttt gaacgttcgc gggcttgctc gacgtagtaa
    37081 gtgacaccac cgccgctcat ccgcgtttct gccgggttat ttccgaacat aacgaccagt
    37141 ttcgtattgg cgatatcatc cgggctgttg ccatcattgg caccgaacat ataactcatt
    37201 gcggcactga tctgtgcggt actgtagctg ccatagcgac tgagaaaacc cccgcaagag
    37261 ttcatcagac ggtacgggac gttcgagttg gtgatatttc cgccatctac gcctgttccg
    37321 tacaggacat gtacagcctc attgccgtaa tctttcagga tccgccgaag attatcactg
    37381 atggtatcca gggcttcgtc ccaacttatc cgttcaaatt taccttcacc gcgctcgccg
    37441 acgcgcttca tgggatattt caacctgtca ggatgattca tccgtcggcg gatagagcgc
    37501 ccgcgtaaac atgcccgaac ctgatgatta ccgtaaacgt cgtcacctgt cgtatcagac
    37561 tccacccagt acacggtgtc atctttcaca tgcaaacgta gcagacagcg gctcccgcag
    37621 ttaacggtgc aggaactcca gaccgttttc tcttctaccg gagcctctgc cgcccggacc
    37681 atttgggaaa atggcagagt gaaagcactg cttgccagcg cgagactgcc aagtgcggag
    37741 gttttcatca gacttctatg gctgatttca gccttcatga gcgcctctgt ggtatggatt
    37801 ttcatcatta ctcacttatt gcttttcaaa caaaatgtca tgccagaatt tatggttgta
    37861 gtgggttata ttttttcgat ctcgaccaga ttagtgtgct gcgggtttcc cttcgccagt
    37921 ggtgaagggc gcagagtggt tagcgtattc acacatccgc catggtcgat tttatcgcca
    37981 gacatattgg gctcgtgcca ggctccctgg cccatagcgc taactccagg gagaatgcgt
    38041 ggtgttactt tggctggtag ccgaacttcg ccacgatggt taaacacccg caccatatcg
    38101 ccgttggcaa tcccacgttt ctgtgcatct atagggttga tccacacctc ctgacggcag
    38161 gctgccttca ggacatcaat gttgccgtag gtcgagtgag tacgggattt gtaatggaaa
    38221 ccaaacagtt gcagtgggaa ggttctacgt tcaggggagt cccagccttc aaaggttgag
    38281 gcataaactg gcagtgggct tatcacttca tctttttcca gttcccaggt acgggcaatt
    38341 tccgccagct tgctggaata aatttcaatc ttaccggaag gcgttttaag tggatttgcc
    38401 tcggggtcgt cacgaaatgc tttgtaggcg acaaaatggc cattgggatc tttacgctta
    38461 tagataccca tttttttcag ttcgtcgtaa gacggtaacg ccggatcttt ggcaagcatt
    38521 ttggcgtaca gatgttgtaa ccattgttcc tgcgtgcgac cttctgtgaa cttttgatag
    38581 acgtcaggtc caagacgttt cgcgacttca ctcaggatcc agtaaatcgg tttacgttcg
    38641 aatttttcgc tggtgactgg ctggaggaaa atgagatatc ccatgttacc ggcgtagtcg
    38701 ttaggaataa tatcttcctg ctcaacggtc atcaggtctt ccagcagaat gtcggcatat
    38761 tttgccgatg aggtcataaa gttttcgatg accacaatca tttcgcattt cgattcgtcc
    38821 tgcagaattt catgcgtttt gttgatgtca gaatgctgat taacgagggt atttcccgcg
    38881 tagttccaga tggactccat tgccagacgg atggatggac ttgaagtagt tgactcaatg
    38941 ctacttaagg tcctgatatg actgacatta atcatgataa cactgtatat ccatccagtt
    39001 ctttctctgg gtctccctat cggtatcagc aagtctacac ctcaagccct gacacaaggc
    39061 tgacacaaat caatctactc agcgtagcta agcctcagtt agtccgcaga gccaatggtc
    39121 gatatactat ccgcttcaga ctcaagggac agaccacacc gtttctttca gtatcgaccc
    39181 gcagcacaga caggagagta gccacaatgc gtcagagaga gcttgcagcc accgctaagg
    39241 ccttcatgtt ggataggcct gaagtctcac tacaggagct tacagagcat ctccggtcta
    39301 tggctgagca gttcctgaca gacgctagtg acgattactg gaatggactt gaggtcgcta
    39361 ctctggtcga cgagaagtcc aacctgaagg agctagccgc tacgcaagcc ttgagtctgg
    39421 accagcagaa gggcataagg ttagcgctgg aagtcctgac cgcagcacag cagagagtcg
    39481 acactggtga tacctctggt ctgattaagc tgattgatga taataacctt actgatgact
    39541 caactatagg cgactctacg tcaattctga ataatgagca gggggataga ccggcagttt
    39601 tcacacagga gcgccagtca tctgtagttt tttcaagcct agtgtcctcg ctgctggctg
    39661 agaaggtcca gaccctgaag acatccagct acaaggacct ctcgtcctca ctcaatactg
    39721 tctcccggtt cctgcctgaa gacatggacc tgatgtcccg ctctgggtgg ctggctgtca
    39781 gggactctat gctggcttct gaggtgagac catcgacaat caacaagctg ctgaccaaag
    39841 cgaagatgtg tctcgattat ggcctgatga atgggcaact tgagggccgc aaccctattg
    39901 aacggatgaa gctgaccaag gatatcgact ccaagcgcag agcgttcaca gacgaggagc
    39961 tggagagact tctggtccgt gttgagtctg
//
LOCUS       DAJTRC010000026        39985 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.026, whole genome shotgun sequence.
ACCESSION   DAJTRC010000026 DAJTRC010000000
VERSION     DAJTRC010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39985
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.026"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(72..1325)
                     /gene="amiC"
                     /locus_tag="OO357_001496"
     CDS             complement(72..1325)
                     /gene="amiC"
                     /locus_tag="OO357_001496"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiC"
                     /protein_id="HCR5886489.1"
                     /translation="MSGSNTAISRRRLLQGAGAMWLLSVSQVSLAAVSQVVAVRVWPA
                     SSYTRVTVESNRQLKYKQFALSNPERVVVDIEDVNLNSVLKGMAAQIRADDPFIKSAR
                     VGQFDPQTVRMVFELKQNVKPQLFALAPVAGFKERLVMDLYPANAQDMQDPLLALLED
                     YNKGDLEKQVPPAQSGPQPGKAGRDRPIVIMLDPGHGGEDSGAVGKYKTREKDVVLQI
                     ARRLRSLIEEEGNMKVYMTRNEDIFIPLQVRVAKAQKQRADLFVSIHADAFTSRQPSG
                     SSVFALSTKGATSTAAKYLAQTQNASDLIGGVSKSGDRYVDHTMFDMVQSLTIADSLK
                     FGKAVLNKLGKINKLHKNQVEQAGFAVLKAPDIPSILVETAFISNVEEERKLKTATFQ
                     QEVAESILAGIKAYFADGATLARRG"
     gene            1557..2888
                     /gene="argA"
                     /locus_tag="OO357_001497"
     CDS             1557..2888
                     /gene="argA"
                     /locus_tag="OO357_001497"
                     /EC_number="2.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708609.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino-acid N-acetyltransferase"
                     /protein_id="HCR5886490.1"
                     /translation="MVKERKTELVEGFRHSVPYINTHRGKTFVIMLGGEAIEHENFSS
                     IVNDIGLLHSLGIRLVVVYGARPQIDANLAAHHHEPLYHKNIRVTDAKTLELVKQAAG
                     TLQLDITARLSMSLNNTPLQGAHINVVSGNFIIAQPLGVDDGVDYCHSGRIRRIDEDA
                     LHRQLESGAIVLMGPVAVSVTGESFNLTSEEIATQLAIKLKAEKMIGFCSSQGVTNDD
                     GDIVSELFPNEAQARVEAQEEKGDYNSGTVRFLRGAVKACRSGVRRCHLISYQEDGAL
                     LQELFSRDGIGTQIVMESAEQIRRATINDIGGILELIRPLEQQGILVRRSREQLEMEI
                     DKFTIIQRDNTTIACAALYPFPEEKIGEMACVAVHPDYRSSSRGEVLLERIAAQAKQS
                     GLSKLFVLTTRSIHWFQERGFTPVDIDLLPESKKQLYNYQRKSKVLMADLG"
     gene            complement(2950..4776)
                     /gene="recD"
                     /locus_tag="OO357_001498"
     CDS             complement(2950..4776)
                     /gene="recD"
                     /locus_tag="OO357_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit alpha"
                     /protein_id="HCR5886491.1"
                     /translation="MKLQKQLLEAVEHKQLRPLDVQFALTVAGDEHPAVTLAAALLSH
                     DAGEGHVCLPLSRLENNEASNPLLATCVSEIGELQNWEECLLASQAVSRGDEPTPMIL
                     CGDRLYLNRMWCNERTVARFFNEVNHTIEVDEALLAQTLDKLFPVSDEINWQKVAAAV
                     ALTRRISVISGGPGTGKTTTVAKLLAALIQMADGERCRIRLAAPTGKAAARLTESLGK
                     ALRQLPLTDEQKKRIPEDASTLHRLLGAQPGSQRLRHHAGNPLHLDVLVVDEASMIDL
                     PMMSRLIDALPDHARVIFLGDRDQLASVEAGAVLGDICAYANAGFTAERARQLSRLTG
                     THVPAGTGTEAASLRDSLCLLQKSYRFGSDSGIGQLAAAINRGDKTAVKTVFQQDFTD
                     IEKRLLQSGEDYIAMLEEALAGYGRYLDLLQARAEPDLIIQAFNEYQLLCALREGPFG
                     VAGLNERIEQFMQQKRKIHRHPHSRWYEGRPVMIARNDSALGLFNGDIGIALDRGQGT
                     RVWFAMPDGNIKSVQPSRLPEHETTWAMTVHKSQGSEFDHAALILPSQRTPVVTRELV
                     YTAVTRARRRLSLYADERILSAAIATRTERRSGLAALFSSRE"
     gene            complement(4776..8318)
                     /gene="recB"
                     /locus_tag="OO357_001499"
     CDS             complement(4776..8318)
                     /gene="recB"
                     /locus_tag="OO357_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit beta"
                     /protein_id="HCR5886492.1"
                     /translation="MSDVAETLDPLRLPLQGERLIEASAGTGKTFTIAALYLRLLLGL
                     GGSAAFPRPLIVEELLVVTFTEAATAELRGRIRSNIHELRIACLRETTDNPLYERLLE
                     EIDDKAQAAQWLLLAERQMDEAAVFTIHGFCQRMLNLNAFESGMLFEQQLIEDESLLR
                     YQACADFWRRHCYPLPREIAQVVFETWKGPQALLRDINRYLQGEPPVIKAPPPDDETL
                     ASRHAQIVARIDTVKQQWRDAVGELDALIESSGIDRRKFNRSNQAKWIEKISAWAEEE
                     TNSYQLPESLEKFSQRFLEDRTKAGGETPRHPLFEAIDQLLAEPLSIRDLLITRALAE
                     IRETVAREKRRRGELGFDDMLSRLDSALRSESGEVLAAAIRTRFPVAMIDEFQDTDPQ
                     QYRIFRRIWHHQPETALLLIGDPKQAIYAFRGADIFTYMKARSEVHAHYTLDTNWRSA
                     PGMVNSVNKLFSQTDDTFMFREIPFIPVKSAGKNQALRFVFKGETQPAMKMWLMEGES
                     CGVGDYQSTMAQVCAAQIRDWLQAGQRGEALLMNGDDARPVRASDISVLVRSRQEAAQ
                     VRDALTLLEIPSVYLSNRDSVFETLEAQEMLWLLQAVMTPERENTLRSALATSMMGLN
                     ALDIETLNNDEHAWDAVVEEFDGYRQIWRKRGVMPMLRALMSARNIAENLLATAGGER
                     RLTDILHISELLQEAGTQLESEHALVRWLSQHILEPDSNASSQQMRLESDKHLVQIVT
                     IHKSKGLEYPLVWLPFITNFRVQDQAFYHDRHSFEAVLDLNAAPESVDLAEVERLAED
                     LRLLYVALTRSVWHCSLGVAPLVRRRGDKKGDTDVHQSALGRLLQKGEPQDAAGLRTC
                     IEALCDDDIAWQTAQTGDNQPWQVNDALTAELNARTLQRLPGDNWRVTSYSGLQQRGH
                     GIAQDLMPRLDVDAAGVVSVVEEPTLTPHQFPRGASPGTFLHSLFEDLDFTQPVDPNW
                     VQEKLELGGFESQWEPVLTEWITAVLQAPLNETGVSLSQLSDRDKQVEMEFYLPISEP
                     LIASQLDALIRQFDPLSAGCPPLEFMQVRGMLKGFIDLVFRHEGRYYLLDYKSNWLGE
                     DSSAYTQQAMAAAMQAHRYDLQYQLYTLALHRYLRHRIADYDYDLHFGGVIYLFLRGV
                     DKEHPQQGIYTTRPNAGLIALMDEMFAGMTLEEA"
     gene            complement(8311..11199)
                     /gene="ptrA"
                     /locus_tag="OO357_001500"
     CDS             complement(8311..11199)
                     /gene="ptrA"
                     /locus_tag="OO357_001500"
                     /EC_number="3.4.24.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin"
                     /protein_id="HCR5886493.1"
                     /translation="MPRSTWFKALLLFVALWAPLSQAETGWQPIQETIRKSDKDNRQY
                     QAIRLDNGMVVLLVSDPQAVKSLSALVVPVGSLEDPEAYQGLAHYLEHMSLMGSKKYP
                     QADSLAEYLKMHGGSHNASTAPYRTAFYLEVENDALPGAVDRLADAIAEPLLDKKYAE
                     RERNAVNAELTMARTRDGMRMAQVSAETINPAHPGSKFSGGNLETLSDKPGNPVQQAL
                     KDFHEKYYSANLMKAVIYSNKPLPELAKMAADTFGRVPNKESKKPEITVPVVTDAQKG
                     IIIHYVPALPRKVLRVEFRIDNNSAKFRSKTDELITYLIGNRSPGTLSDWLQKQGLVE
                     GISANSDPIVNGNSGVLAISASLTDKGLANRDQVVAAIFSYLNLLREKGIDKQYFDEL
                     ANVLDIDFRYPSITRDMDYVEWLADTMIRVPVEHTLDAVNIADRYDAKAVKERLAMMT
                     PQNARIWYISPKEPHNKTAYFVDAPYQVDKISAQTFADWQKKAADIALSLPELNPYIP
                     DDFSLIKSEKKYDHPELIVDESNLRVVYAPSRYFASEPKADVSLILRNPKAMDSARNQ
                     VMFALNDYLAGLALDQLSNQASVGGISFSTNANNGLMINANGYTQRLPQLFQALLEGY
                     FSYTATEEQLEQAKSWYNQMMDSAEKGKAFEQAIMPAQMLSQVPYFSRDERRKILPSI
                     TLKEVLAYRDALKSGARPEFMVIGNMTEAQATTLARDVQKQLGADGSEWCRNKDVVVD
                     KKQSVIFEKAGNSTDSALAAVFVPTGYDEYTSSAYSSLLGQIVQPWFYNQLRTEEQLG
                     YAVFAFPMSVGRQWGMGFLLQSNDKQPSFLWERYKAFFPTAEAKLRAMKPDEFAQIQQ
                     AVITQMLQAPQTLGEEASKLSKDFDRGNMRFDSRDKIVAQIKLLTPQKLADFFHQAVV
                     EPQGMAILSQISGSQNGKAEYVHPEGWKVWENVSALQQTMPLMSEKNE"
     gene            complement(11375..14743)
                     /gene="recC"
                     /locus_tag="OO357_001501"
     CDS             complement(11375..14743)
                     /gene="recC"
                     /locus_tag="OO357_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease V subunit gamma"
                     /protein_id="HCR5886494.1"
                     /translation="MLRVYHSNRLDVLEALMEFIVERERLDDPFEPEMILVQSTGMAQ
                     WLQMTLSQKFGIAANIDFPLPASFIWDMFVRVLPEIPKESAFNKQSMSWKLMTLLPQL
                     LEREDFTLLRHYLTDDSDKRKLFQLSSKAADLFDQYLVYRPDWLAQWETGHLVEGLGE
                     AQAWQAPLWKALVEYTHELGQPRWHRANLYQRFIETLESATTCPPGLPSRVFICGISA
                     LPPVYLQALQALGKHIEIHLLFTNPCRYYWGDIKDPAYLAKLLTRQRRHSFEDRELPL
                     FRDSENAGQLFNSDGEQDVGNSLLASWGKLGRDYIYLLSDLESSQELDAFVDVTPDNL
                     LHNIQSDILELENRAVAGVNIEEFSRSDNKRPLDPLDSSITFHVCHSPQREVEVLHDR
                     LLAMLEEAPTLTPRDIIVMVADIDSYSPFIQAVFGSAPADRYLPYAISDRRARQSHPV
                     LEAFISLLSLPDSRFVSEDVLALLDVPVLAARFDITEEGLRYLRQWVNESGIRWGIDD
                     DNVRELELPATGQHTWRFGLTRMLLGYAMESAQGEWQSVLPYDESSGLIAELVGHLAS
                     LLMQLNIWRRGLAQERPLEEWLPVCRDMLNAFFLPDAETEAAMTLIEQQWQAIIAEGL
                     GAQYGDAVPLSLLRDELAQRLDQERISQRFLAGPVNICTLMPMRSIPFKVVCLLGMND
                     GVYPRQLAPLGFDLMSQKPKRGDRSRRDDDRYLFLEALISAQQKLYISYIGRSIQDNS
                     ERFPSVLVQELIDYIGQSHYLPGDEALNCDESEARVKAHLTCLHTRMPFDPQNYQPGE
                     RQSYAREWLPAASQAGKAHSEFVQPLPFTLPETVPLETLQRFWAHPVRAFFQMRLQVN
                     FHTEDSEIPDTEPFILEGLSRYQINQQLLNALVEQDDAERLFRRFRAAGDLPYGAFGE
                     IFWETQCQEMQQLADRVIACRQPGQSMEIDLACNGVQITGWLPQVQPDGLLRWRPSLL
                     SVAQGMQLWLEHLVYCASGGNGESRLFLRKDGEWRFPPLAAEQALHYLSQLIEGYREG
                     MSAPLLVLPESGGAWLKTCYDAQNDAMLDDDSTLQKARTKFLQAYEGNMMVRGEGDDI
                     WYQRLWRQLTPETMEAIVEQSQRFLLPLFRFNQS"
     gene            complement(14756..15106)
                     /locus_tag="OO357_001502"
     CDS             complement(14756..15106)
                     /locus_tag="OO357_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708614.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HCR5886495.1"
                     /translation="MSASLKNQQGFSLPEVMVAMVLMVLMVLMVLMVMIVTALSGIQR
                     TSMNSLASRNQYQKLWRHGWQQTQLRAISPPANWQVNRMQTSQAGCVSISVTLVSPGG
                     REGEMTRLHCPNCQ"
     gene            complement(15091..15498)
                     /locus_tag="OO357_001503"
     CDS             complement(15091..15498)
                     /locus_tag="OO357_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2509 family protein"
                     /protein_id="HCR5886496.1"
                     /translation="MNREKGVSSLALVLMLLVLGSLLLQGMSQQDRSFASRVSMESQS
                     LRRQAIVQSALAWGKMHSWQTQPAVQCSQYAGTDAQVCLRLLADNEALLIAGYEGVSL
                     WRTGEVIDGKIVFSPRGWSDFCPLKEGALCQLP"
     gene            complement(15495..16058)
                     /locus_tag="OO357_001504"
     CDS             complement(15495..16058)
                     /locus_tag="OO357_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
                     /protein_id="HCR5886497.1"
                     /translation="MPVKEQGFSLLEVLIAMAISSVLLLGAARFLPALQRESLTSTRK
                     LALEDEIWLRVFTVAKHLQRAGYCHGSCTGEGLEIVGQGDCIIVQWDANSNGIWDREP
                     VKESDQIGFRLKEHVLETLRGATSCEGKGWDKVTNPDAIIIDTFQVVRQDVSGFSPVL
                     TVNMRAASKSEPQTVVDASYSVTGFNL"
     gene            complement(16049..16519)
                     /locus_tag="OO357_001505"
                     /pseudo
     CDS             complement(16049..16519)
                     /locus_tag="OO357_001505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417303.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase-dependent protein"
     gene            complement(16703..17497)
                     /gene="thyA"
                     /locus_tag="OO357_001506"
     CDS             complement(16703..17497)
                     /gene="thyA"
                     /locus_tag="OO357_001506"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HCR5886498.1"
                     /translation="MKQYLELMQKVLDEGTQKNDRTGTGTLSIFGHQMRFNLQDGFPL
                     VTTKRCHLRSIIHELLWFLQGDTNIAYLHENNVTIWDEWADENGDLGPVYGKQWRAWP
                     TPDGRHIDQITTVLNQLKNDPDSRRIIVSAWNVGELDKMALAPCHAFFQFYVADGKLS
                     CQLYQRSCDVFLGLPFNIASYALLVHMMAQQCDLEVGDFVWTGGDTHLYSNHMDQTHL
                     QLSREPRPLPKLIIKRKPESIFDYRFEDFEIEGYDPHPGVKAPVAI"
     gene            complement(17504..18379)
                     /gene="lgt"
                     /locus_tag="OO357_001507"
     CDS             complement(17504..18379)
                     /gene="lgt"
                     /locus_tag="OO357_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HCR5886499.1"
                     /translation="MTSSYLHFPEFDPVIFSIGPVALHWYGLMYLVGFIFAMWLATRR
                     ANRPGSGWTKNEVENLLYAGFLGVFLGGRIGYVLFYNFPQFMADPLYLFRVWDGGMSF
                     HGGLIGVIVVMIIFARRTKRSFFQVSDFIAPLIPFGLGAGRLGNFINGELWGRVDPNF
                     PFAMLFPGSRTEDILLLQTNPQWQSIFDTYGVLPRHPSQLYELLLEGVVLFIILNLYI
                     RKPRPMGAVSGLFLIGYGAFRIIVEFFRQPDAQFTGAWVQYISMGQILSIPMIVAGVI
                     MMVWAYRRSPQQHVS"
     gene            complement(18530..20776)
                     /gene="ptsP"
                     /locus_tag="OO357_001508"
     CDS             complement(18530..20776)
                     /gene="ptsP"
                     /locus_tag="OO357_001508"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HCR5886500.1"
                     /translation="MLTRLREIVEKVASAPRLNEALNILVTDICLAMDTEVCSVYLAD
                     HDRRCYYLMATRGLKKPRGRTVTLAFDEGIVGLVGRLAEPINLADAQKHPSFKYIPSV
                     KEERFRAFLGVPIIQRRQLLGVLVVQQRELRQYDESEESFLVTLATQMAAILSQSQLT
                     ALFGQYRQTRIRALPAAPGVAIAEGWQDATLPLMEQVYQASTLDPALERERLTGALEE
                     AANEFRRYSKRFAAGAQKETAAIFDLYSHLLSDTRLRRELFAEVDKGSVAEWAVKTVI
                     EKFAEQFAALSDNYLKERAGDLRALGQRLLFHLDDANQGPNAWPERFILVADELSATT
                     LAELPQDRLVGVVVRDGAANSHAAIMVRALGIPTVMGADIQPSVLHRRTLIVDGYRGE
                     LLVDPEPVLLQEYQRLISEEIELSRLAEDDVNLPAQLKSGERIKVMLNAGLSPEHEEK
                     LGSCIDGIGLYRTEIPFMLQSGFPSEEEQVAQYQGMLQMFNDKPVTLRTLDVGADKQL
                     PYMPISEENPCLGWRGIRITLDQPEIFLIQVRAMLRANAATGNLNILLPMVTSLDEVD
                     EARRLIERAGREVEEMIGYEIPKPRIGIMLEVPSMVFMLPHLAKRVDFISVGTNDLTQ
                     YILAVDRNNTRVANIYDSLHPAMLRALAMIAREAEIHGIDLRLCGEMAGDPMCVAILI
                     GLGYRHLSMNGRSVARVKYLLRRIDFAEAENLAQRSLEAQLATEVRHQVAAFMERRGM
                     GGLIRGGL"
     gene            complement(20789..21319)
                     /gene="rppH"
                     /locus_tag="OO357_001509"
     CDS             complement(20789..21319)
                     /gene="rppH"
                     /locus_tag="OO357_001509"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001655775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HCR5886501.1"
                     /translation="MIDDDGYRPNVGIVICNRQGQVMWARRFGQHSWQFPQGGINPGE
                     SAEQAMYRELFEEVGLSRKDVRILASTRNWLRYKLPKRLVRWDTKPVCIGQKQKWFLL
                     QLVSGDAEINMQTSSTPEFDGWRWVSYWYPVRQVVSFKRDVYRRVMKEFASVVMSLQE
                     NTPKPQNASAYRRKRG"
     gene            22004..22693
                     /gene="mutH"
                     /locus_tag="OO357_001510"
     CDS             22004..22693
                     /gene="mutH"
                     /locus_tag="OO357_001510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutH"
                     /protein_id="HCR5886502.1"
                     /translation="MSQPRPLLSPPETEEQLLAQAQQLSGYTLGELAALDGLVTPENL
                     KRDKGWIGVLLEIWLGASAGSKPEQDFAALGVELKTIPVDSLGRPLETTFVCVARLTG
                     NSGVTWETSHVRHKLKRVLWIPVEGERSIPLAQRRVGSPLLWSPNEEEDRQLREDWEE
                     LMDMIVLGQVERITARHGEYLQIRPKAANAKALTEAIGARGERILTLPRGFYLKKNFT
                     SALLARHFLIQ"
     gene            22762..23475
                     /locus_tag="OO357_001511"
     CDS             22762..23475
                     /locus_tag="OO357_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5886503.1"
                     /translation="MLFAWITDPNAWLALGTLTLLEIVLGIDNIIFLSLVVAKLPTAQ
                     RAHARRLGLAGAMVMRLALLASISWVTRLTNPLFTIFSQEISARDLILLLGGLFLIWK
                     ASKEIHESIEGEEEGLKTRVSSFLGAIVQIMLLDIIFSLDSVITAVGLSDHLFIMMAA
                     VVIAVGVMMFAARSIGDFVERHPSVKMLALSFLILVGFTLILESFDIHVPKGYIYFAM
                     FFSIAVESLNLIRNKKNPL"
     gene            23613..23831
                     /gene="ygdR"
                     /locus_tag="OO357_001512"
     CDS             23613..23831
                     /gene="ygdR"
                     /locus_tag="OO357_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein YgdR"
                     /protein_id="HCR5886504.1"
                     /translation="MKKWAVIISAVGLAFAVSGCSSDYVMATKDGRMILTDGKPEIDD
                     DTGLVSYHDQQGNAMQINRDDVSQIIER"
     gene            23939..24979
                     /locus_tag="OO357_001513"
     CDS             23939..24979
                     /locus_tag="OO357_001513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP(H)-dependent aldo-keto reductase"
                     /protein_id="HCR5886505.1"
                     /translation="MQYHRIPHSSLEVSTLGLGTMTFGEQNSEADAHAQLDYAVAQGI
                     NLIDVAEMYPVPPRPETQGLTETYVGNWLAKHGSREKLIIASKVSGPSRNNDKGIRPD
                     QALDRKNIREALHDSLKRLQTDYLDLYQVHWPQRPTNCFGKLGYSWTDSAPAVSLLDT
                     LDALAEYQRAGKIRYIGVSNETAFGVMRYLHLADKHDLPRIVTIQNPYSLLNRSFEVG
                     LAEVSQYEGVELLAYSCLGFGTLTGKYLNGAKPAGARNTLFSRFTRYSGEQTQKAVAA
                     YVDIARRHGLDPAQMALAFVRRQPFVASTLLGATTMDQLKTNIESLHLELSEDVLAEI
                     EAVHQVYTYPAP"
     gene            complement(25012..26202)
                     /gene="lplT"
                     /locus_tag="OO357_001514"
     CDS             complement(25012..26202)
                     /gene="lplT"
                     /locus_tag="OO357_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid transporter LplT"
                     /protein_id="HCR5886506.1"
                     /translation="MSESVHTNTSLWSKGMKAVIVAQFLSAFGDNALLFATLALLKAQ
                     FYPEWSQPILQMVFVGAYILLAPFVGQVADSFAKGRVMMFANGLKLLGAASICFGINP
                     FLGYTLVGVGAAAYSPAKYGILGELTTGSKLVKANGLMEASAIAAILLGSVAGGVLAD
                     WHVLVALAACALAYGGAVVANIYIPKLAARPGQSWNLINMTRSFLNACTSLWCNGETR
                     FSLVGTSLFWGAGVTLRFLLVLWVPVALGITDNATPTYLNAMVAIGIVVGAGAAAKLV
                     TLETVSRCMPAGILIGVVVPIFSLQHELLPAYALLMLIGVLGGFFVVPLNALLQERGK
                     KSVGAGNAIAVQNLGENSAMLLMLGIYSLAVMVGIPVVPIGIGFGALFALAITALWIW
                     QRRH"
     gene            complement(26195..28354)
                     /gene="aas"
                     /locus_tag="OO357_001515"
     CDS             complement(26195..28354)
                     /gene="aas"
                     /locus_tag="OO357_001515"
                     /EC_number="2.3.1.40"
                     /EC_number="6.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acyl-ACP--phospholipid
                     O-acyltransferase/long-chain-fatty-acid--ACP ligase"
                     /protein_id="HCR5886507.1"
                     /translation="MLFSFFRNLCRVLYRVRVTGDTQALKGERVLITPNHVSFIDGIL
                     LGLFLPVRPVFAVYTSISQQWYMRWLKSFIDFVPLDPTQPMAIKHLVRLVEQGRPVVI
                     FPEGRITTTGSLMKIYDGAGFVAAKSGATVIPVRIEGAELTHFSRLKGLVKRRLFPQI
                     TLHILPPTQVAMPDAPRARDRRKIAGEMLHQIMMEARMAVRPRETLYESLLSAMYRFG
                     AGKKCVEDVNFTPDSYRKLLTKTLFVGRILEKYSVEGERIGLMLPNAGISAAVIFGAI
                     ARRRIPAMMNYTAGVKGLTSAITAAEIKTIFTSRQFLDKGKLWHLPEQLTQVRWVYLE
                     DLKADVTTADKVWIFAHLLMPRLAQVKQQPEEEALILFTSGSEGHPKGVVHSHKSILA
                     NVEQIKTIADFTTNDRFMSALPLFHSFGLTVGLFTPLLTGAEVFLYPSPLHYRIVPDL
                     VYDRSCTVLFGTSTFLGHYARFANPYDFYRLRYVVAGAEKLQESTKQLWQDKFGLRIL
                     EGYGVTECAPVVSINVPMAAKPGTVGRILPGMDARLLSVPGIEEGGRLQLKGPNIMNG
                     YLRVEKPGVLEVPTAENVRGEMERGWYDTGDIVRFDEQGFVQIQGRAKRFAKIAGEMV
                     SLEMVEQLALGVSPDKVHATAIKSDASKGEALVLFTTDNELTRDKLQQYAREHGVPEL
                     AVPRDIRYLKQMPLLGSGKPDFVTLKSWVDEAEQHDE"
     gene            28940..29971
                     /gene="galR"
                     /locus_tag="OO357_001516"
     CDS             28940..29971
                     /gene="galR"
                     /locus_tag="OO357_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator GalR"
                     /protein_id="HCR5886508.1"
                     /translation="MATIKDVARLAGVSVATVSRVINNSPKASEASRLAVHSAMESLS
                     YHPNANARALAQQTTETVGLIVGDVSDPFFGAMVKAVEQVAYHTGNFLLIGNGYHNEQ
                     KERQAIEQLIRHRCAALVVHAKMIPDADLASLMKQMPGMVLINRILPGFENRCIALDD
                     RYGAWLATRHLIQQGHTRIGYLCSNHSISDAEDRLQGYYDALAESGIPANDRLVTFGE
                     PDESGGEQAMTELLGRGRNFTAVACYNDSMAAGAMGVLNDNGIDVPGEISLIGFDDVL
                     VSRYVRPRLTTVRYPIVTMATQAAELALALADNRPLPEITNVFSPTLVRRHSVSTPSL
                     EASHHATSD"
     gene            complement(29978..31240)
                     /gene="lysA"
                     /locus_tag="OO357_001517"
     CDS             complement(29978..31240)
                     /gene="lysA"
                     /locus_tag="OO357_001517"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HCR5886509.1"
                     /translation="MPHSLFSTDTDLTAENLLRLPAEFGCPVWVYDAQIIRRQIAALK
                     QFEVVRFAQKACSNIHILRLMREQGVKVDSVSLGEIERALAAGYNPQTHPDDIVFTAD
                     VIDQATLERVSELQIPVNAGSVDTLDQLGQVSPGHRVWLRVNPGFGHGHSQKTNTGGE
                     NSKHGIWYTDLPAALDVIQRHHLQLVGIHMHIGSGVDYAHLEQVCGAMVRQVIEFGQD
                     LQAISAGGGLSIPYQQGEEAVDTEHYYGLWNAAREQIARHLGHPVKLEIEPGRFLVAQ
                     SGVLITQVRSVKQMGSRHFVLVDAGFNDLMRPAMYGSYHHISALAADGRSLEHAPTVE
                     TVVAGPLCESGDVFTQQEGGNVETRALPEVKAGDYLVLHDTGAYGASMSSNYNSRPLL
                     PEVLFDNGQARLIRRRQTIEELLALELL"
     gene            31362..32297
                     /gene="lysR"
                     /locus_tag="OO357_001518"
     CDS             31362..32297
                     /gene="lysR"
                     /locus_tag="OO357_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator LysR"
                     /protein_id="HCR5886510.1"
                     /translation="MAAVNLRHIEIFHAVMTAGSLTEAAHLLHTSQPTVSRELARFEK
                     VIGLKLFERVRGRLHPTVQGLRLFEEVQRSWYGLDRIVSAAESLREFRQGELSIACLP
                     VFSQSFLPQLLQPFLARYPDVSLNIVPQESPLLEEWLSAQRHDLGLTETLHTPTGTER
                     TELLSLDEVCVLPPGHPLAVKKVLTPDDFQGENYISLSRTDSYRQLLDQLFTEHQVKR
                     RMIVETHSAASVCAMVRAGVGISVVNPLTALDYAASGLVVRRFSIAVPFTVSLIRPLH
                     RPSSALVQAFSGHLQAGLPKLVTSLDAILSSATTA"
     gene            complement(32284..32976)
                     /gene="ygeA"
                     /locus_tag="OO357_001519"
     CDS             complement(32284..32976)
                     /gene="ygeA"
                     /locus_tag="OO357_001519"
                     /EC_number="5.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HCR5886511.1"
                     /translation="MKTIGLLGGMSWESTIPYYRLINEGIKQRLGGLHSAQMLLHSVD
                     FHEIEECQRRGEWDKTGDILAEAALGLQRAGAEGIVLCTNTMHKVADAIESRCTLPFL
                     HIADATGRAITGAGMTRVALLGTRYTMEQDFYRGRLTEQFSINCLIPEADERAKINQI
                     IFEELCLGQFTEASRAYYAQVIARLAEQGAQGVIFGCTEIGLLVPEERSVLPVFDTAA
                     IHAEDAVAFMLS"
     gene            complement(33105..34523)
                     /gene="araE"
                     /locus_tag="OO357_001520"
     CDS             complement(33105..34523)
                     /gene="araE"
                     /locus_tag="OO357_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-proton symporter AraE"
                     /protein_id="HCR5886512.1"
                     /translation="MVTINTESALTPRSLRDTRRMNMFVSVAAAVAGLLFGLDIGVIA
                     GALPFITDHFVLTSRLQEWVVSSMMLGAAIGALFNGWLSFRLGRKYSLMAGAILFVLG
                     SIGSAFATSVEMLIAARVVLGIAVGIASYTAPLYLSEMASENVRGKMISMYQLMVTLG
                     IVLAFLSDTAFSYSGNWRAMLGVLALPAVLLIILVVFLPNSPRWLAEKGRHIEAEEVL
                     RMLRDTSEKAREELNEIRESLKLKQGGWALFKINRNVRRAVFLGMLLQAMQQFTGMNI
                     IMYYAPRIFKMAGFTTTEQQMIATLVVGLTFMFATFIAVFTVDKAGRKPALKIGFSVM
                     ALGTLVLGYCLMQFDNGTASSGLSWLSVGMTMMCIAGYAMSAAPVVWILCSEIQPLKC
                     RDFGITCSTTTNWVSNMIIGAAFLTLLDSIGAAGTFWLYTALNIAFVGITFWLIPETK
                     NVTLEHIERKLMAGEKLRNIGV"
     gene            complement(34838..35599)
                     /gene="kduD"
                     /locus_tag="OO357_001521"
     CDS             complement(34838..35599)
                     /gene="kduD"
                     /locus_tag="OO357_001521"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HCR5886513.1"
                     /translation="MILSAFSLQGKVAVVTGCDTGLGQGMALGLAQAGCDIVGINIVE
                     PTETIEQVTALGRRFLSLTADLRKIDGIPGLLDRAVAEFGHIDILVNNAGLIRREDAL
                     EFSEKDWDDVMNLNIKSVFFMSQAAAKHFIAQGNGGKIINIASMLSFQGGIRVPSYTA
                     SKSGVMGVTRLMANEWAKHNINVNAIAPGYMATNNTQQLRADEQRSAEILDRIPAGRW
                     GLPSDLMGPVVFLASSASDYVNGYTIAVDGGWLAR"
     gene            complement(35629..36465)
                     /gene="kduI"
                     /locus_tag="OO357_001522"
     CDS             complement(35629..36465)
                     /gene="kduI"
                     /locus_tag="OO357_001522"
                     /EC_number="5.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
                     /protein_id="HCR5886514.1"
                     /translation="MDVRQSIHGAHAKTLDTQGLRNEFLVEKVFVADEYTMVYSHIDR
                     IIVGGIMPITKTVSVGGEVGKQLGVSYFLERRELGVINIGGAGTITVDGQCYEIGHRD
                     ALYVGKGAKEVVFASIDTATPAKFYYNCAPAHTTYPTKKVTPDEVSPVTLGDNLTSNR
                     RTINKYFVPDVLETCQLSMGLTELAPGNLWNTMPCHTHERRMEVYFYFNMDDDACVFH
                     MMGQPQETRHIVMHNEQAVISPSWSIHSGVGTKAYTFIWGMVGENQVFNDMDHVGVKD
                     LR"
     gene            complement(36752..37933)
                     /locus_tag="OO357_001523"
     CDS             complement(36752..37933)
                     /locus_tag="OO357_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HCR5886515.1"
                     /translation="MKDVVIVGALRTPIGCFRGALAGHSAVELGSLVVKALIERTGVP
                     AYAVDEVILGQVLTAGAGQNPARQSAIKGGLPNSVSAITINDVCGSGLKALHLATQAI
                     QCGEADIVIAGGQENMSRAPHVLTDSRTGEQLGNSQLVDSLVHDGLWDAFNDYHIGVT
                     AENLAREYGISRQLQDAYALSSQQKARAAIDAGRFKDEIVPVMIQSNGQTLVVDTDEQ
                     PRTDASAEGLARLNPSFDSLGSVTAGNASSINDGAAAVMMMSEAKARALNLPVLARIR
                     AFASVGVDPALMGIAPVYATRRCLERVGWQLADVDLIEANEAFAAQALSVGKMLEWDE
                     RRVNVNGGAIALGHPIGASGCRILVSLVHEMVKRNARKGLATLCIGGGQGVALTIERD
                     E"
     gene            38188..39417
                     /locus_tag="OO357_001524"
                     /pseudo
     CDS             38188..39417
                     /locus_tag="OO357_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311729.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
BASE COUNT         9923 a        10813 c         9701 g         9548 t
ORIGIN      
        1 attgaacacc ctaacgggtg tttttttgtt tctgatctcc cataaaaaaa gcgccattca
       61 gcgccttttt atcatcccct tctcgccagc gttgccccat cggcaaaata cgctttaatc
      121 cccgcaagaa tagactccgc aacttcctgc tggaaagtcg ccgttttcag tttacgctct
      181 tcctcaacgt tactgataaa cgccgtttcg accagaatgg agggaatatc tggtgccttt
      241 agtacggcaa acccggcctg ttcaacttga tttttatgca gcttgttgat ttttccgagc
      301 ttattcagta ccgctttacc aaacttaagg ctgtcagcaa tagtcagcga ctgcaccata
      361 tcgaacatgg tgtggtcgac atagcggtca ccgcttttgc tcacgccacc aatcaagtcc
      421 gaggcgttct gggtttgtgc cagatatttt gccgcagtac tggttgcgcc tttggttgag
      481 agcgcaaaca ccgaggaacc gctcggctga cgactggtaa aggcgtcggc atggatagag
      541 acaaacaggt cagcacgctg tttctgggct tttgctacgc gcacttgcaa cggaatgaag
      601 atgtcttcat tgcgcgtcat gtacaccttc atattgccct cttcctcgat cagagagcgc
      661 aaacggcgag ctatttgcaa taccacatct ttttcgcgcg ttttgtattt ccccaccgca
      721 ccggagtctt cgccaccgtg gccagggtca agcataatga caatcggacg atcgcgccct
      781 gctttacccg gttgtggacc actttgcgct ggcggcacct gcttttcgag gtcgcctttg
      841 ttgtaatcct ccagcagcgc cagcagcggg tcctgcatat cctgtgcatt ggccggatag
      901 aggtccatca ccagacgctc tttaaacccg gcgaccggcg caagggcaaa cagctgcggt
      961 tttacgtttt gctttaattc aaaaaccatg cgtacggttt gcgggtcaaa ttgcccgacg
     1021 cgcgccgact tgatgaacgg gtcgtctgca cgaatttgtg ccgccatccc cttgagtacc
     1081 gagttcaggt ttacatcttc gatatccacc accacgcgtt cagggttact caacgcgaac
     1141 tgcttatatt tcagctgacg atttgattct accgtcacgc gggtgtagct ggacgcaggc
     1201 cagacgcgca ccgccacgac ctggctaacc gcagccaggc tgacctgact tacgctcaat
     1261 agccacatag cgcccgcgcc ttgcagtaaa cgacgacggc tgattgcagt gttggatcct
     1321 gacatgcctc tcccgagcaa aagaaatcta agctgtgtaa caagtaaacg actaatttga
     1381 ccggaaactt tagcgaaaga cgcataatct gtcatctaat aaacggtaaa cattcttttt
     1441 atattcacgg cattactgat aaaaaagtcg ctctcgcata aaatttacac ttgcaccctg
     1501 cgaaaaaaca gaataaaaat acaataattt caaataatca tgcaaagggg tgtgccgtgg
     1561 taaaggaacg taaaaccgag ctggtcgagg gattccgcca ttcggttccc tatatcaata
     1621 cccaccgggg aaaaacgttt gtcatcatgc tcggcggtga agccattgag catgagaatt
     1681 tctccagtat cgttaatgat atcgggttgt tgcacagcct cggcatccgt ctggtggtgg
     1741 tctatggcgc acgtccgcag atcgacgcaa atctggctgc gcatcaccac gaaccgctgt
     1801 atcacaagaa tatacgtgtg accgacgcca aaacactgga actggtgaag caggctgcgg
     1861 gaacattgca actggatatt actgctcgcc tgtcaatgag tctcaataac acgccgctgc
     1921 agggcgcgca tatcaacgtc gttagtggca attttattat tgcccagccg ctgggcgtcg
     1981 atgacggcgt ggattactgc catagcgggc gtatccggcg gattgatgaa gacgcgctcc
     2041 atcgtcaact ggaaagcggt gcaatagtgc taatggggcc ggtcgctgtt tcagtcactg
     2101 gcgagagctt taatctgacc tcggaagaga ttgccactca actagccatc aaactgaaag
     2161 ctgaaaagat gattggtttt tgctcttccc agggcgttac taatgacgac ggtgatattg
     2221 tctccgaact tttccctaac gaagcgcaag cacgggtaga agcccaggaa gagaaaggcg
     2281 attacaactc cggtacggtg cgctttttgc gtggcgcagt gaaagcctgc cgcagcggcg
     2341 tgcgccgctg tcatttaatc agttatcagg aagatggcgc gctgttgcaa gagttgttct
     2401 cacgcgacgg tatcggtacg cagattgtga tggaaagcgc cgagcagatt cgtcgcgcaa
     2461 caatcaacga tattggcggt attctggagt tgattcgccc gctggagcag caaggtattc
     2521 tggtgcgccg ttctcgcgag cagctggaga tggaaatcga caaattcacc attattcagc
     2581 gcgataacac gactattgcc tgcgccgcgc tctatccatt cccggaagag aagattgggg
     2641 aaatggcctg tgtggcagtt cacccggatt accgcagttc atcacggggc gaggttctgc
     2701 tggaacgcat tgccgctcag gcgaagcaga gcggcttaag caaattgttt gtgctgacca
     2761 cgcgcagtat tcactggttc caggaacgtg gatttacccc ggtggatatt gatttactgc
     2821 ccgagagcaa aaagcagttg tacaactacc agcgtaaatc caaagtgttg atggcggatt
     2881 tagggtaagc gatgaaaatc accggatgcg gcaggcgtaa cactcgtgcg tcgcatccgg
     2941 caattacgtt tattcccgcg aactaaacaa cgccgccaga ccactgcgcc gctcagtacg
     3001 agtggcgatt gccgcactta atatacgctc atcggcatac agcgacagac gacgacgcgc
     3061 gcgggtcacc gcggtgtata ccagctctcg cgttactacc ggcgtgcgtt ggctcggtaa
     3121 aatcaacgcc gcatggtcga actccgatcc ctgcgattta tgtaccgtca tcgcccacgt
     3181 agtttcgtgc tctggcaagc gactcggttg cacagactta atattgccgt ccggcatcgc
     3241 aaaccagacg cgcgtcccct gcccgcgatc cagcgcaata ccgatatcgc cattaaacaa
     3301 cccaagcgcg ctgtcattac gggcaatcat caccggtcgg ccttcgtacc aacgagagtg
     3361 cggatgacga tgaattttgc gcttctgttg cataaactgt tcaattcgct cattcagtcc
     3421 agccacgcca aacggccctt cccgcagggc gcacaaaagc tggtactcat tgaacgcctg
     3481 aatgattaaa tccggctcgg cacgcgcttg cagcagatcc agataacgtc cgtaacccgc
     3541 aagagcttcc tcaagcatcg caatataatc ttcgccgctc tgtaaaagtc gtttttcgat
     3601 atcagtaaaa tcctgctgaa aaacggtttt cactgccgtt ttatcaccac ggttgatcgc
     3661 cgcagctaac tgaccaatgc cagaatcgct gccgaaacga tagctttttt gcagcaggca
     3721 gagactgtcg cgcaaagatg ccgcttctgt gccggttcct gccggaacgt gagtccccgt
     3781 caggcggctt agctgcctgg cacgctcggc ggtaaagccc gcgttggcat aagcgcagat
     3841 atcgcccagc acagccccag cctcaaccga ggccagttga tcacgatcgc cgagaaagat
     3901 cactcgcgca tgatctggca aggcgtcgat cagtctcgac atcataggca gatcgatcat
     3961 tgacgcttca tctaccacca gcacatcaag atgcagcggg ttaccggcat gatgacgtaa
     4021 acgctggcta cccggctgcg cgcccagcaa tcggtgcaaa gtgctggcat cttccggaat
     4081 gcgtttcttt tgttcatcgg tcagcggtaa ctgtcgcaaa gccttgccga gagattcggt
     4141 taagcgcgcg gcagctttac ccgttggtgc agccagacgg atacggcagc gttcgccgtc
     4201 ggccatttga attaacgctg ccagcaactt cgccacggtg gtcgttttac cggtgcctgg
     4261 gccgccggaa atcaccgaga tccgccgcgt cagcgccact gccgctgcaa ctttttgcca
     4321 gttaatttca tcgcttactg gaaaaagttt gtccagggtt tgcgccagta gagcttcatc
     4381 aacctcaatg gtatgattca cctcattgaa gaagcgcgct accgtccgct cgttacacca
     4441 catgcgattc aaataaagac gatcgccaca gaggatcatc ggcgtgggtt catctcccct
     4501 gctgaccgct tgagaagcca gcaagcattc ttcccaattt tgtagctcac cgatttcact
     4561 gacacaggtc gccaacagcg gattcgacgc ctcgttattt tccagtcgtg aaagcggcaa
     4621 acagacgtgt ccctctccgg catcatgact taacagtgcc gccgcgaggg tgacggcagg
     4681 atgttcatct cccgccacgg tcagggcaaa ttgcacatcc agcgggcgta gctgtttgtg
     4741 ctccacagct tccagtaatt gcttttgcaa tttcattacg cctcctccag ggtcataccg
     4801 gcaaacatct catccatcag ggcaatcaac ccggcgttgg gtcgggttgt gtaaatccct
     4861 tgttgcggat gttctttatc aacgccacgc aggaacagat agataacgcc gccaaagtga
     4921 agatcatagt cgtaatcagc aatgcgatgg cgcagataac gatgcagcgc cagggtataa
     4981 agctgatatt gcagatcata gcggtgtgcc tgcatcgctg ctgccatagc ctgttgggtg
     5041 taagccgaac tgtcttcacc cagccagttg gatttatagt cgagcaggta ataacgccct
     5101 tcgtggcgga ataccaggtc gataaagcct tttaacatgc cgcgtacctg catgaactcc
     5161 agcggcgggc agccagcgga tagcgggtca aactggcgaa ttagtgcatc aagctggctg
     5221 gcgatgagcg gttcgctaat cggcaggtaa aactccatct ccacctgttt atcgcggtcg
     5281 gaaagctgac tcaggctaac acccgtttca ttgagaggtg cctggaggac agccgtgatc
     5341 cactcggtca acaccggttc ccactgcgat tcaaagccgc cgagttccag tttttcctgt
     5401 acccagttcg ggtcaaccgg ctgggtaaaa tcgaggtctt caaacaaact gtgcaagaat
     5461 gtccccggtg acgcaccgcg cgggaactga tgcggtgtta acgtcggttc ttcaacgacg
     5521 ctgaccacgc ctgcggcatc aacatccagc cgtggcatca gatcctgagc gataccgtga
     5581 ccacgctgct gcaagccgga gtagctggtg acacgccagt tatcgccggg caatcgttgt
     5641 agtgtcctcg cattcagttc tgcagttaaa gcatcattaa cctgccaggg ctggttatca
     5701 ccagtttgtg ccgtttgcca ggcaatatca tcatcgcata acgcttcaat acaggtgcga
     5761 agccctgccg catcttgcgg ttcccctttt tgcagcaaac gcccgagcgc actttggtgg
     5821 acgtcggtgt cacctttttt atcgccacga cggcgcacca gcggtgcaac gccgagactg
     5881 caatgccaaa ccgaacgcgt cagcgccacg taaagcaaac gcagatcttc cgccagacgt
     5941 tcgacctccg cgaggtcgac gctttctggc gcagcattaa gatccaaaac tgcctcaaac
     6001 gagtggcgat cgtgataaaa cgcctgatcc tggacgcgga aattggtaat aaatggcaac
     6061 cagaccagcg gatattccag ccctttcgat ttatggatcg tgacaatctg caccagatgt
     6121 ttatcacttt cgaggcgcat ttgttggctg gaggcattac tgtctggctc gaggatatgt
     6181 tgcgataacc agcgtaccag cgcatgttca ctttccagct gcgttccggc ttcttgaagc
     6241 agttcgctga tatgcaagat atcggtaaga cgccgctcac cgcctgccgt tgccagcaag
     6301 ttttctgcaa tgttacgcgc cgacatcagc gcccgcagca tcggcataac gccacgtttg
     6361 cgccagattt gccgataacc atcgaactct tcgactaccg catcccacgc atgttcgtca
     6421 ttattcagcg tttcaatatc cagcgcattc agccccatca ttgacgttgc cagcgcactg
     6481 cgcagggtgt tctcacgttc gggcgtcatc accgcctgca acaaccaaag catttcctgc
     6541 gcttccagag tttcaaaaac actgtcgcgg ttcgaaaggt aaacggaagg gatttccagc
     6601 aacgttaagg catcgcgcac ctgggcggcc tcctggcggc tgcgcaccag cacgctgatg
     6661 tccgaagcac gcaccggacg cgcgtcgtcg ccgttcatca gcaacgcttc gccccgctgt
     6721 ccggcttgta gccagtcgcg gatttgcgca gcacatacct gcgccatggt actttgatag
     6781 tcgccaacgc cgcagctttc gccttccatc agccacattt tcatcgcagg ctgcgtttca
     6841 cctttaaata caaaacgtaa cgcctgattt ttcccggctg atttcactgg aataaacggt
     6901 atttcgcgaa acatgaacgt gtcatcagtc tggctgaaaa gcttgttcac actgttcacc
     6961 attccaggtg cggagcgcca gttggtgtct aaagtgtagt gggcatgaac ttcactacgc
     7021 gccttcatat aagtgaagat atccgcaccc cggaatgcat atatggcctg cttcgggtcg
     7081 ccaatcagca acaatgcggt ttccggctga tggtgccaga tacggcgaaa aattcggtac
     7141 tgctgggggt cggtatcctg aaattcatcg atcattgcca ccgggaatcg cgtacggatc
     7201 gccgctgcca acacctcgcc gctttcgcta cgcagcgcgg aatcgagccg acttaacatg
     7261 tcatcaaaac ccaattcgcc acggcggcgt ttttcacgcg ctactgtttc gcggatctca
     7321 gccaatgcgc gggtgatcaa cagatcgcgg atcgacaatg gttctgcaag cagttgatcg
     7381 atcgcctcaa acagtggatg tcgcggggtt tcccccccgg ccttcgtgcg atcttctaag
     7441 aaacgctggg agaatttttc cagcgactcc ggcaactgat aactgtttgt ctcttcttct
     7501 gcccaggcgc tgatcttttc gatccattta gcctgattgc tacggttaaa cttgcgtcga
     7561 tcaataccag aagattcgat cagcgcatcc agttcaccca ctgcgtcgcg ccactgctgt
     7621 tttaccgtat caatacgcgc cacaatttgc gcgtggcggg aagccagcgt ttcatcatcg
     7681 ggcggcggtg ctttgataac cggcggttcg ccttgcagat aacgattaat atcgcgcagc
     7741 aacgcctgcg gccctttcca ggtttcaaag acgacctggg ctatttcacg cggcagcggg
     7801 tagcagtggc gacgccagaa atcggcgcag gcctggtagc gtagcagaga ctcatcttca
     7861 atcagctgct gctcaaacag catgccggat tcaaaggcat tcaggttgag catgcgctgg
     7921 caaaagccgt gaatagtaaa gactgccgct tcatccatct gccgttcggc taacaacaac
     7981 cactgcgcgg cttgcgcttt atcgtcgatc tcttccagca ggcgttcgta cagtggattg
     8041 tcggtggttt cacgcagaca ggcgatgcgc aactcgtgga tattgctacg gatacgaccg
     8101 cgcaattctg ccgtggcagc ctcggtaaag gtgaccacca gcagttcttc aacgatcagc
     8161 gggcggggaa aggcggcgga accgcctagt ccaagtaaca ggcgcaaata gagcgccgca
     8221 atcgtaaagg ttttgcctgt gccggcagag gcttcaatca ggcgctcgcc ctgtaagggc
     8281 aagcgcaaag gatctagtgt ctcggcgaca tcactcattc ttttcactca tcaggggcat
     8341 tgtttgctgc aacgcgctga cgttctccca cactttccag ccttcaggat gcacatattc
     8401 ggctttcccg ttctggctgc cggaaatctg cgacagaata gccatgcctt gcggctcgac
     8461 caccgcctga tggaagaaat cagcaagttt ttgcggcgtc agcagtttta tctgggccac
     8521 gattttatca cgcgaatcga agcgcatatt gccgcgatcg aaatctttac ttaacttcga
     8581 tgcttcttcg ccgagcgttt gcggtgcctg cagcatctgg gtaattaccg cctgctggat
     8641 ttgcgcaaac tcatctggct tcatcgctcg caattttgcc tctgcggttg ggaaaaacgc
     8701 cttgtaacgc tcccacaaga atgaaggctg tttatcattg ctttgcaaaa ggaagcccat
     8761 gccccactga cgccccacgc tcattggaaa cgcaaacacg gcatagccca attgttcttc
     8821 ggtacgcaac tgattgtaga accacggctg tacgatctgc cccaacagag agctataggc
     8881 tgagctggtg tattcatcgt agccagtcgg tacaaatacc gctgccagtg cggagtcggt
     8941 gctgttaccg gctttttcaa agatgacgga ttgtttttta tcgaccacta catctttgtt
     9001 tcgacaccac tctgaaccat cagcgcccaa ctgtttttgc acatcgcgtg ccagcgttgt
     9061 tgcctgggcc tcggtcatgt tgccgataac cataaactct ggtcgagccc ctgattttaa
     9121 ggcgtcgcga taggccagca cctctttcaa cgtaatggag ggcaaaattt tacgccgttc
     9181 atctcgcgag aagtacggca cttgcgagag catctgcgcg ggcataatcg cctgctcaaa
     9241 cgctttaccc ttttctgcgg aatccatcat ctggttatac caggacttcg cctgctcaag
     9301 ctgctcttcc gtagcggtat agctaaagta cccctcgagc aatgcctgga acagctgcgg
     9361 cagacgctgg gtgtaaccat tagcattaat cataaggccg ttgttagcgt tggtggaaaa
     9421 acttatgcca ccaaccgacg cctggttgct taactgatca agcgccagcc ctgcgagata
     9481 atcattgagc gcaaacatca cctgattgcg ggcgctgtcc atggctttcg gattacgcaa
     9541 aatcaggctg acatcagctt tgggctcgct ggcaaaataa cggcttggcg catacaccac
     9601 gcgcagattc gactcatcaa caatcagctc tggatggtcg tatttcttct ctgacttaat
     9661 cagcgagaaa tcatcaggaa tataagggtt aagctctggc aaagagagcg caatgtcggc
     9721 ggcttttttc tgccagtcgg cgaaagtttg tgcgctgatt ttatcgacct gatacggcgc
     9781 atcgacaaag taagccgttt tgttgtgcgg ctctttcggg ctgatatacc agatacgcgc
     9841 attctgcggc gtcatcatcg ccagacgttc ctttactgct ttagcatcgt accgatcggc
     9901 aatattgact gcatccagcg tatgctcaac aggaacgcga atcatggtat ctgccagcca
     9961 ttcgacgtaa tccatatcac gggtgatcga cggataacgg aagtcgatat ccagcacatt
    10021 cgccagttca tcgaagtatt gtttatcaat gcctttttca cgtaacagat tgagatagct
    10081 aaaaattgcc gccacaacct gatcgcgatt agccaggcct ttatcagtta aagacgcaga
    10141 gatcgctaat acgccgctgt tgccgttgac gataggatcg gagttggcgc taatgccctc
    10201 aactaatccc tgcttttgca gccagtcaga aagtgtacct gggctgcgat tgccaatcag
    10261 ataggtaatc aattcatcgg ttttactacg gaacttcgct gagttgttat cgatgcgaaa
    10321 ctcaacgcgc aacactttac gcggcagcgc agggacgtaa tgaatgataa tgcccttttg
    10381 cgcgtcggtg actaccggca cggtgatttc cggttttttg ctctctttgt tcggcacgcg
    10441 accaaaggtg tccgccgcca tttttgccaa ctccggcagc ggtttattac tgtaaataac
    10501 cgccttcatc aaattggcgg aatagtactt ctcgtggaaa tctttcagcg cctgctgcac
    10561 cggattacca ggtttgtcgc ttaaagtttc gaggttacca ccagaaaact ttgaaccggg
    10621 gtgtgccggg ttaatggttt ctgcgctgac ctgtgccatg cgcatcccgt cacgcgtacg
    10681 cgccatggtt aattcagcgt tcactgcatt acgctcacgt tcggcgtatt tcttgtcgag
    10741 caagggttct gcaatagcat cggccaggcg gtctaccgca ccgggcaagg cgtcgttctc
    10801 aacttccaga tagaaagccg tgcgatacgg cgcagtgctg gcattgtgac taccgccgtg
    10861 cattttgaga tattcggcca gactgtcagc ctgcgggtac tttttcgacc ccatcagact
    10921 catatgttca aggtaatgtg ccagcccctg gtacgcctcg ggatcttcca gcgacccaac
    10981 gggcactacc agcgccgaga gcgatttaac tgcctgcgga tcagaaacca gcaagaccac
    11041 cataccgtta tccagacgta tagcctgata ctggcggtta tctttatcac ttttacggat
    11101 ggtttcctga atcggctgcc atcccgtttc tgcctgactt aatggtgccc agagggcaac
    11161 aaacaacaat aatgctttga accaggtgct gcggggcatt cacggacctc ataatcaact
    11221 ttattttctg gacagattca acaggttaac gcaaatcatc tgccagaatt taatcttgtg
    11281 ctgcacgagc cagcctatgt ttatataacc atcagtccgt gactggtgcg catcataaag
    11341 taagcagata gattgcgcaa tttttataca acactcatga ctgattaaag cgaaacagcg
    11401 gtaacaggaa acgttgcgac tgttcaacga tggcctccat tgtctctggt gttaattgcc
    11461 gccagagccg ttgataccag atatcatcac cttcgccacg caccatcatg ttgccttcgt
    11521 aagcctgaag gaatttcgta cgggcttttt gcaacgtgga atcgtcatcc agcatggcat
    11581 cgttttgcgc gtcataacag gtttttagcc acgcgccgcc actttcaggt aacaccagca
    11641 atggcgcgga cattccttca cgatacccct caatcagttg tgagaggtaa tgcaaagcct
    11701 gttcggctgc aagcggcgga aaacgccact caccgtcttt gcgtagaaaa aggcgacttt
    11761 cgccattacc accgctggca cagtagacaa ggtgttccag ccaaagttgc attccctgcg
    11821 ccacacttaa taaagaggga cgccagcgca acaggccatc cggctgcacc tgcggcaacc
    11881 agccagttat ctgcacaccg ttgcaggcga gatcaatttc catactctgc cccggctggc
    11941 gacaggcaat gactctgtcg gcaagctgct gcatctcctg gcactgtgtt tcccagaaaa
    12001 tttcaccaaa agcgccatac ggtaaatccc ctgccgcccg gaagcggcgg aacaagcgtt
    12061 cggcatcatc ctgctcaacc agtgcattca ataactgctg attgatttga taacggctaa
    12121 gtccttccag aataaatggc tcggtgtctg ggatttcgct gtcttcagta tggaagttca
    12181 cctgcaaacg catctggaag aacgcccgca ccggatgtgc ccagaatcgt tgtagcgttt
    12241 ccagcggcac ggtttccggt aaggtaaacg gcagcggctg aacaaattca gaatgtgctt
    12301 taccagcctg gctggccgca ggtagccatt cacgagcata gctttgtcgt tcgcctggct
    12361 ggtagttttg cggatcaaac ggcatccggg tatggaggca agtaagatgc gcttttaccc
    12421 ttgcctcgct ttcatcacag ttgagcgctt catcgcccgg tagataatga ctttgcccga
    12481 tgtagtcgat cagttcctgc accagtaccg acgggaaacg ttcactgtta tcctgaatgg
    12541 aacgcccgat atagctgata tagagttttt gctgcgcgga aattaacgct tccaggaaca
    12601 ggtagcggtc gtcatcgcga cggctacggt cgccacgctt cggtttctgg ctcatcaggt
    12661 caaagcccaa tggcgcaagc tgacgtggat aaacgccgtc gttcattccc agcaggcaaa
    12721 ccactttgaa cggaattgaa cgcattggca tcagagtaca aatgttaacc ggtccggcga
    12781 gaaaacgctg gctgatacgt tcttgatcca gacgctgtgc cagttcatca cgcaatagtg
    12841 acagcggcac cgcgtcgcca tactgcgcac ctaaaccttc ggcgataatc gcctgccatt
    12901 gttgttcgat cagcgtcatc gccgcttcgg tttccgcatc cggcaggaag aaggcgttga
    12961 gcatatcgcg acaaaccggc aaccactctt ctagcggacg ctcctgtgcc agcccgcgac
    13021 gccagatgtt tagctgcatt agcagtgaag ccagatgccc caccagttct gcaattaagc
    13081 cgctcgattc atcataaggt agaaccgatt gccactcgcc ctgcgcgctc tccatcgcgt
    13141 agcccagcaa catacgcgtc aggccaaatc gccaggtgtg ttgtccggtg gcggggagtt
    13201 ccagctcgcg aacgttgtcg tcatctatcc cccaacgaat gccggattcg ttgacccact
    13261 ggcgtaaata acgcagccct tcttcggtga tgtcaaaccg cgccgccagc accggcacat
    13321 ccagcagcgc cagcacatcc tctgacacaa agcgactgtc aggcagtgat aacaggctga
    13381 taaacgcttc cagtaccgga tgtgactgcc gcgcacgacg gtcggaaatg gcgtaaggca
    13441 ggtaacgatc cgcaggtgcg ctaccaaaca ctgcctgaat aaacggactg tagctgtcga
    13501 tatcagccac catcacgatg atgtcgcgcg gagtaagtgt cggggcttcc tccagcatcg
    13561 ccagcaagcg atcgtgtaaa acttcaactt cacgctgcgg gctatggcaa acgtggaatg
    13621 tgatactgct atccagtgga tcaagcgggc gtttgttatc gctacgggaa aactcttcga
    13681 tgttcacacc cgcaacggcg cggttttcca gttccagaat gtcagactga atgttatgca
    13741 gcaggttatc tggcgtcaca tcgacaaaag cgtccagctc ctggctgctc tccaggtcag
    13801 aaaggagata aatgtagtcg cgcccaagct tcccccatga ggccagcagc gagttgccga
    13861 catcctgttc accatcgctg ttaaagagct gtccggcatt ttcgctatcg cgaaatagcg
    13921 gcaattcgcg atcttcaaaa ctgtgtcgac gctgacgagt cagcagtttc gccagataag
    13981 caggatcttt aatatcgccc cagtaataac ggcaggggtt ggtaaacagg agatggattt
    14041 caatatgttt acccagcgcc tggagcgcct ggagataaac aggcggtaac gcggaaatac
    14101 cgcatataaa gacgcgcgaa ggtaaccccg gcgggcaggt cgtcgcggac tccagcgttt
    14161 cgataaagcg ctgatagaga ttcgcgcggt gccagcgcgg ttgtccgagt tcatgggtat
    14221 attccaccag cgccttccac aacggggctt gccaggcctg tgcttctccc agcccttcaa
    14281 ccaaatgtcc tgtttcccac tgtgccagcc agtccggacg atagaccaga tactggtcaa
    14341 acaggtccgc cgcttttgag gaaagctgga acagttttcg cttgtcgcta tcgtcagtca
    14401 gataatgccg caacagggta aagtcttcgc gctccagcaa ttgcggcagc agagtcatca
    14461 gtttccagct catgctctgt ttgttaaagg cgctctcttt ggggatttcc ggtaacaccc
    14521 ggacgaacat atcccagata aagctcgctg gcagcggaaa atcaatgttt gccgcaatac
    14581 caaacttttg cgacagggtc atttgcagcc actgcgccat accggtactt tgcaccagaa
    14641 tcatctctgg ttcgaaagga tcgtccagcc gttcgcgttc gacaataaac tccatcaacg
    14701 cttccagcac gtccagacga ttggaatggt agacccttaa catagcggct cctgactact
    14761 gacaattcgg gcaatgcagg cgggtcatct cgccttctct gcccccgggt gaaactagcg
    14821 taacgctgat gctgacacat cccgcctgcg atgtctgcat tcggttgacc tgccagttgg
    14881 caggtggcga aatcgcgcgc agttgcgttt gctgccaacc atgccgccag agcttttggt
    14941 actggtttct gctggcgaga ctgttcattg atgttcgctg gatacccgat aacgcagtga
    15001 caatcatcac catcagcacc atcagcacca tcagcaccat cagcaccatc gcaaccatta
    15061 cctccggcag gctaaagcct tgttgattct tcagggaagc tgacataatg ccccctcttt
    15121 cagcggacaa aaatcgctcc agccgcgtgg cgaaaaaaca atttttccat caatgacttc
    15181 gcctgttcgc cacaacgaaa cgccttcata accggcaatc aataaggctt cattatctgc
    15241 cagtaaacgc aaacaaacct gggcatcggt tccagcgtac tgcgagcact gaactgctgg
    15301 ctgcgtctgc caggagtgca tttttcccca cgccagcgcc gactgaacga tggcctggcg
    15361 gcgcaatgac tgactttcca tgctcacgcg agaggcaaaa ctgcgatcct gctgactcat
    15421 tccttgcaat agcaagctac ccaaaaccag cagcatcagg accagagcca gtgacgaaac
    15481 acctttttcg cggttcacag gttgaatcct gtcacgctat agctggcatc caccacggtt
    15541 tgcggttcag acttactggc agcacgcata ttaaccgtca acaccggcga gaagccgctg
    15601 acatcctgac gtacgacctg aaaagtgtcg ataatgatgg catccggatt agtgacttta
    15661 tcccagccct taccttcaca ggatgtcgca ccgcgtagcg tttccagcac atgctccttc
    15721 agacgaaatc caatctggtc ggactctttt accggttcgc gatcccagat accgttactg
    15781 ttcgcatccc actgcacaat gatacagtca ccctgtccga caatttccag cccttcgccg
    15841 gtacagctgc catgacaata acccgccctc tggagatgct tcgcgacggt aaatacccgc
    15901 agccagattt catcttccag tgccagctta cgagtgctcg ttaaactttc acgctgtaac
    15961 gcaggcagaa agcgtgcagc ccccagcaac aatacgctac tgatcgccat agcaatcaac
    16021 acttccagca gagaaaaacc ttgctctttt acaggcatcc ttctgtttct ccttgctgat
    16081 aaagccggag tcttccccac ggcgaaacca ccagccacca ctcgcccgtt gagtttttga
    16141 agcgaatatg cacggcccat gcggtattgc gcaggccaaa gaaagcaagc gaaggtgtca
    16201 ggtcgctcat ttcgacttcg ggccagcgtg gcacaaagac caatggtgaa ctgccatgac
    16261 aggtattggc cccagcagcg gaactcacaa ggcaccataa cgtcccctcc ctgataacgc
    16321 tgatactgtg gtcgcggtta tgccagttgg catcttcacg taaatagagc aaatagtccc
    16381 gcgcctagct ggcggtttgc caaagccgtt gcgactgctg ccagtattgc cagccataga
    16441 gtccacttgc gcttagcatg accaaaatca gcatcgcgac cagcgtttca atcagcgtat
    16501 aaccacgttg tgttttcatg ccggcagtat ggagcgagga gaaaaaaaga cgagggccag
    16561 tttctatttc ttcggcgcat cttccggact atttacgccg ttgcaggacg ttgcaaaatt
    16621 tcgggaaggc gtctcgaaga atttaacgga gggtaaaaaa accgacgcac actggcgtcg
    16681 gctctggcag gatgtttcgt aattagatag ccaccggcgc tttaacgccc ggatgtggat
    16741 cgtagccttc aatctcaaag tcttcgaaac ggtagtcgaa gatggattcg ggtttacgtt
    16801 tgataatcaa cttcggcagc ggacgcggtt cgcggcttaa ttgcagatga gtttgatcca
    16861 tatggttgct gtacaggtgc gtatcgccac cggtccagac aaaatcaccc acttccagat
    16921 cgcactgctg cgccatcata tgcaccagta acgcgtagct ggcaatgttg aacggcaggc
    16981 cgaggaagac gtcacaggag cgctgataaa gctggcaaga gagtttgccg tctgccacat
    17041 agaactggaa gaatgcatgg cacggtgcca gcgccatttt atccagttcg cctacgttcc
    17101 acgctgaaac aataatgcgg cgcgaatccg ggtcgttttt cagctggttc agtaccgtag
    17161 taatctggtc aatatgacga ccatctggcg ttggccaggc gcgccactgt ttaccataca
    17221 ctggcccgag gtcgccattt tcatcggccc attcgtccca gatggtgaca ttgttttcgt
    17281 gtagataagc aatgttagta tcgccctgca ggaaccacag cagttcgtgg atgatggaac
    17341 gcaggtggca acgtttggtt gtcaccagcg ggaatccatc ctgcaggtta aaacgcatct
    17401 gatgaccaaa aatggaaagc gttccggttc cggtacggtc gtttttctgt gtgccttcgt
    17461 cgagcacttt ttgcatcagt tctaaatact gtttcatggt tcctcaggaa acgtgttgct
    17521 gtgggctgcg acgatatgcc cagaccatca tgatcacacc cgcgacaatc atcgggatgg
    17581 aaagaatttg ccccatgctg atgtactgca cccaggcacc ggtaaactgc gcgtcgggct
    17641 ggcggaaaaa ctcaacaatg atgcgaaacg cgccgtagcc aatcaggaac aagcctgaga
    17701 cagctcccat tgggcgcggt ttacgaatat acaggttgag gataataaac agcaccacac
    17761 cttccagcag cagttcgtaa agctgtgatg ggtggcgcgg cagcacaccg taagtgtcga
    17821 aaatggattg ccactgcggg ttggtttgca gcagcaaaat atcttctgta cgggagcctg
    17881 ggaacagcat ggcaaacggg aagttcgggt caacgcggcc ccacaattca ccgttaataa
    17941 agttgcccag acgcccggca ccaagaccaa acggaatgag tggtgcgata aaatcagaga
    18001 cctggaagaa ggaacgttta gtacggcggg cgaagataat catcaccacg ataacgccaa
    18061 tcaggccgcc gtggaaagac atgccgccgt cccagacacg gaacagatac agcggatcgg
    18121 ccataaactg cgggaaattg tagaacagaa cataaccaat acgtcccccg aggaagacgc
    18181 cgaggaagcc cgcatagagt aagttttcaa cttcattttt ggtccagcca ctgcccggac
    18241 gattcgcccg acgtgttgcc agccacattg caaaaatgaa ccccaccaga tacatcaggc
    18301 cgtaccagtg aagcgccacg ggtcctattg agaaaatgac cggatcaaac tccggaaaat
    18361 gcagatagct actggtcatc tgtcaccaca agttcttgtt atttcgctga aagagaacag
    18421 cgattgaaat gcgcgccgca ggtttcaggc gctccaaagg tgcgaataat agcacaaggg
    18481 gacctggctg gttgccggat accgttaaaa gatatgtata tgatccgcgc tataaccctc
    18541 cgcgaatcag cccgcccatg ccgcgacgct ccataaaggc tgcaacctga tgacgaactt
    18601 cggtcgccag ttgcgcttcc agactacgct gcgcaagatt ttctgcttcg gcaaaatcaa
    18661 tgcgccgcag caggtatttt acccgcgcta cagaacgtcc gttcatagac agatggcgat
    18721 acccaagccc aatgaggatt gccacgcaca tgggatcgcc cgccatttca ccgcacaaac
    18781 gaagatcgat accatgtatt tccgcttccc gggcgatcat cgccagagct cgtaacattg
    18841 caggatgaag actgtcgtaa atgttcgcca cccgggtatt gttgcgatca acggccagaa
    18901 tgtattgagt cagatcgttg gtgccaacag agatgaaatc gaccctcttt gccagatgcg
    18961 gcagcataaa taccattgac ggcacttcca gcatgatgcc gatacgtggt ttgggaattt
    19021 cgtaaccgat catctcctcg acttcacgtc cggcacgttc aatcaggcgg cgtgcttcgt
    19081 caacttcatc gaggctggtg accatcggca acagaatatt caggttgccc gtagcggcat
    19141 tagcacgcag catcgcccgc acctggatca agaagatctc cggctgatcg agcgtaatgc
    19201 gaatcccacg ccaacccagg catggattct cttcgctgat cggcatgtaa ggcagctgct
    19261 tatctgctcc gacatccagc gtacgcaagg tgacgggttt atcattgaac atttgcagca
    19321 tcccctgata ctgcgccacc tgttcttctt ccgacgggaa accactttgc agcatgaatg
    19381 ggatttcagt gcgataaaga ccgatgccat caatacagct gcccagtttt tcttcatgtt
    19441 ccgggcttaa accagcattg agcatgactt ttatacgctc accgcttttt aactgcgcgg
    19501 gtaaattgac gtcatcttcc gccagacggc taagctcaat ctcttcacta attagccgct
    19561 gatattcttg cagcagtacc ggctccggat cgaccagcaa ttcaccgcga tagccatcaa
    19621 cgatcagcgt cctacgatgc agcaccgaag gctgaatatc cgcgcccatc acggtaggga
    19681 tccccagcgc acgtaccatg atcgcagcat gggagttggc tgcgccatct cgcacgacaa
    19741 caccgactaa gcgatcctgg ggcagctcag caagagttgt cgctgacagt tcatctgcca
    19801 ccagaatgaa acgttccggc caggcgttcg gcccttgatt agcgtcatca agatgaaaca
    19861 gcaatcgctg acccagcgca cgtaaatcac cagcccgctc tttgagatag ttatcgctta
    19921 gcgcggcaaa ctgttcggca aatttttcaa tgaccgtttt taccgcccac tctgccaccg
    19981 agcctttatc aacctcggca aacaattcgc gacgcagccg ggtgtccgaa agcaggtgcg
    20041 agtaaagatc gaaaatagcc gccgtttctt tttgtgctcc ggcggcaaag cgtttgctgt
    20101 agcggcgaaa ctcgtttgcc gcttcttcca gcgccccggt cagtcgttcg cgttccagag
    20161 ccggatccag cgttgatgcc tgatacacct gttccattaa aggtaacgtg gcatcctgcc
    20221 agccttcggc aatcgccaca ccaggtgctg ccggtaatgc gcggattcgc gtctggcgat
    20281 attgcccaaa cagggcagtc aactgcgact gagaaagaat agctgccatc tgggtggcaa
    20341 gcgtcaccag gaaggattct tcactttcgt catactggcg caactctcgt tgctgtacca
    20401 ccagtacacc aagcaactgg cgacgttgaa taattggtac gcctaaaaac gcgcggaaac
    20461 gttcttcttt tacggagggg atgtatttga agctggggtg cttttgcgca tctgcaaggt
    20521 ttatcggttc cgccagcctg ccaaccaggc cgacgatccc ttcatcaaac gcgagcgtta
    20581 cagtgcgacc gcgtggtttt ttcagccccc gggtcgccat caggtagtaa caacgtcgat
    20641 catgatcggc caggtagacc gaacagacct cggtatccat cgcaagacag atgtcggtaa
    20701 ccagaatatt taacgcctca ttcaggcgtg gtgcgctggc taccttttcg actatttcgc
    20761 gcaggcgagt gagcataatt ggcgtgactt aacctctttt acgtcgataa gcagatgcgt
    20821 tttgtggttt tggcgtattt tcctgcagtg acatcaccac actcgcgaac tctttcatta
    20881 ccctacggta gacatcacgt ttaaatgaca ccacctgtct gaccggatac cagtaactta
    20941 cccatcgcca gccgtcaaac tctggtgtac tgctggtttg catattgatt tctgcatcgc
    21001 cgcttaccag ctgcaagaga aaccattttt gtttttggcc gatacaaacc ggcttcgtgt
    21061 cccaacgcac caaacgtttc ggtaatttgt agcgcaacca gttacgcgtt gaagcaagga
    21121 ttcgaacgtc tttgcggctt aatcctactt cttcaaacaa ttcacggtac atcgcctgct
    21181 ctgcggattc tccggggttg attccgcctt gcggaaattg ccaggagtgc tgaccaaatc
    21241 gccgggccca cattacctgc ccctggcgat tacaaatcac gatacctacg tttgggcggt
    21301 agccatcgtc atcaatcacc ggactacctc aaaataaagc tttatatacg aatgattgtt
    21361 tcatactcca ggaagacggt aaaccactct ctgcagggca ttacacacta ataacaattg
    21421 aataactcac agttatgtgc agagttataa acagaggaag gggtggatag ccgtttttat
    21481 tcaccttttc tgtggataga gttgtgaaga actacggaat tactacggga aaacccggag
    21541 cattctgaat aagcccgata tagacatttt aaaatattca ttattagtca atgcattaca
    21601 tgtaatttga gtaatgaaaa agagtaattc gtgacccagg tcacacctct catttacggg
    21661 ttggcgaaag atcaaccaat gccgtgttta tccacagaat gtgccactaa gttaagcact
    21721 gaaccactaa aaactggagt ttcgtcgcac gtcaaggctg taaatgaaaa cagtagtgga
    21781 ggtttttcac agttatccca gctttctgtg gataacatgg tgtaagatcc tgtttatttt
    21841 cagtgaccag atttggaaaa cctattgcag tgttgcgcaa ctcgattacc ggcagcctaa
    21901 aaagcaatat aaatcagaga attgaacaac acatgtggaa aaagttacac tgcgaatatt
    21961 cggcacataa ttgctgtttg ttttttaacc aaggtatcat gacatgtccc aacctcgccc
    22021 actgctctct cctcccgaaa ctgaagaaca gttgttagcg caagcacagc aactttctgg
    22081 ttatacattg ggagaactgg cggcacttga cgggctggtt acgccagaga atttaaaacg
    22141 cgataagggc tggattggcg tgttactgga gatctggcta ggtgccagcg cagggagtaa
    22201 acctgagcaa gattttgctg ctctgggcgt ggaacttaaa actatccctg tggatagtct
    22261 tggtcgtccg ctggaaacaa cattcgtttg tgttgcccgg ttaacgggta atagcggggt
    22321 gacctgggaa accagccatg tgcgccacaa gctcaaacgc gtactgtgga taccggttga
    22381 aggcgagcgc agcatcccgc tggcgcagcg tcgcgtagga tcaccgttgc tgtggagccc
    22441 gaatgaagag gaagaccgac agctacgcga agactgggaa gaattaatgg atatgattgt
    22501 tctcggtcag gttgagcgga tcaccgctcg tcacggggaa tatttacaga taagaccgaa
    22561 agcagcgaat gcgaaagcgc ttaccgaagc cattggtgcc cggggcgaac ggattctgac
    22621 gctgccacgc ggcttttatt tgaagaagaa tttcaccagt gcactactgg cccgtcattt
    22681 tctgatccag tagccatcgc tttgacctgc cgctttccgg gcatataatt accgcttcat
    22741 ttttttggca gggcttttta gatgttattt gcatggataa ccgatcctaa cgcttggctt
    22801 gcgctcggta cgctgacgct gctggagatc gttcttggga tcgacaatat tattttcctt
    22861 tctctggtgg tggcaaagct tcccacagca caacgtgctc atgcgcgccg tctggggttg
    22921 gcgggagcca tggttatgcg tctggcgctg ctggcatcaa tctcttgggt tacgcgcctg
    22981 acaaatccgc tttttacaat attcagtcag gaaatttctg cccgtgattt gattctgctt
    23041 ctgggtggct tgttccttat ctggaaagcc agcaaggaaa tccacgaatc cattgaaggt
    23101 gaagaagaag ggctgaaaac acgcgtttca tcattcctcg gcgctatcgt gcagattatg
    23161 ctgctggata ttatcttcag cctcgactcg gtgattaccg ctgtgggtct gtcagatcac
    23221 ctgtttatta tgatggcagc cgtggtgatt gccgtaggcg tgatgatgtt cgccgcgcgc
    23281 tcgattggtg attttgtcga acgccatcct tcggtaaaaa tgctggcgct ctctttcctg
    23341 attctggtgg gctttaccct gattctggaa agtttcgaca tccacgtacc gaaaggttac
    23401 atctacttcg cgatgttctt ctctattgcg gttgaaagcc tcaacttgat tcgcaacaaa
    23461 aagaatccgc tctgatactc cgtacgctct cctgcgggag ggcgtactca ctccttttac
    23521 cctcacagtt taagattttc ctgctttcaa gatatataac gtcggtttat aaacagacta
    23581 ttatcatagg taggccaaat gaggataacc ggatgaaaaa atgggcagta ataatttctg
    23641 cagtcggact ggcgtttgct gtttccgggt gttccagtga ttacgtcatg gcgaccaaag
    23701 atggccgtat gattttgacc gatggaaaac ctgaaattga tgatgatacc gggctggtga
    23761 gttatcacga tcagcaaggt aacgcgatgc aaattaaccg tgatgatgtt tcgcaaatta
    23821 ttgaacgtta acaaataagg tcagcatccg gctggcctta agatttttct ctttcccttt
    23881 tcccttccct ctgccatttt tatattcctt atgtcgtgat tataaaaagg aaacggctat
    23941 gcaatatcac cgtatacccc acagttcgct ggaagtcagc acgctggggc ttggcacgat
    24001 gacgtttggt gaacagaaca gcgaagccga cgcccacgca caactcgact atgccgtcgc
    24061 tcagggcatt aaccttatcg acgttgccga aatgtaccca gtacctccgc gccccgaaac
    24121 gcaagggtta accgaaacct acgtcggcaa ctggctggcg aaacatggca gccgcgaaaa
    24181 gttaattatc gcctccaaag tgagcggacc gtcgcgcaat aatgacaagg gcatccgccc
    24241 ggatcaggcg ctggatcgga agaatatccg cgaagcgctg catgacagcc tcaagcgcct
    24301 acagactgat tacctcgatc tttatcaggt gcactggccg cagcgcccga ccaactgctt
    24361 cggcaaactc ggttatagct ggacggattc tgcgcctgcg gtttcgctgc tggatacgct
    24421 ggacgcactg gcagagtacc aacgcgcggg aaaaattcgt tatatcggcg tgtcgaacga
    24481 aactgcattt ggcgtaatgc gctacctgca tctggcggac aaacacgatc tgccgcgtat
    24541 tgtcaccatt cagaaccctt acagtctgtt aaaccgcagt tttgaagtag gtctggcaga
    24601 agtcagccag tatgaagggg tcgaactgct ggcctattcg tgcctgggtt tcggcacgct
    24661 gaccgggaaa tatctcaatg gtgcaaaacc cgctggcgca cgtaatacgc tctttagtcg
    24721 gttcacccgc tatagcggtg agcaaacgca aaaagccgtc gcggcgtatg ttgatatcgc
    24781 cagacgtcat ggccttgatc ctgctcagat ggcgctcgcg tttgtacgcc gtcaaccgtt
    24841 tgttgccagc actctgctgg gcgcaaccac gatggatcag ctgaaaacta acatcgaaag
    24901 tttgcatctg gagttaagcg aagacgtatt agctgaaatt gaagcggtac atcaggttta
    24961 tacttatcct gcaccataaa gattaacgcc ggttaaaacc ggcgttaaat attaatgacg
    25021 gcgctgccag atccacagcg ccgttattgc cagcgcaaat agcgcaccga agccaatgcc
    25081 aatgggcacg accgggatgc ctaccattac cgccagcgag taaatgccca gcatcaacaa
    25141 catggcgctg ttttcgccaa ggttttgtac tgcaatcgca ttccccgccc cgacgctttt
    25201 tttaccccgc tcctgtagca acgcattgag cggaacgaca aaaaagcccc ccagcacgcc
    25261 aatcagcatc aacaaggcat aggctggcag cagctcgtgt tgcagggaga aaatcggtac
    25321 caccacgcca atcaaaatcc cggctggcat acagcgtgac acggtttcca gcgtaaccag
    25381 cttcgctgcc gcacctgcgc caaccacgat accaatcgct accatcgcgt tgagataggt
    25441 cggcgtagcg ttatcggtaa tgcccagcgc caccggtacc cacagcacca acaggaaacg
    25501 cagcgtgaca cccgctcccc agaataaact ggtgcccacc agcgaaaaac gcgtttcacc
    25561 attgcaccat agcgaggtgc aggcattcag gaaactgcgg gtcatgttga tgagattcca
    25621 ggactgcccc ggacgcgccg ccagtttggg aatgtagata ttggcaacga ccgcaccacc
    25681 gtaggccagt gcgcatgcgg ccagagcgac caggacatgc cagtcagcca gcacaccacc
    25741 ggctacggaa ccgagtaaaa tcgccgctat ggcagaagct tccattaaac cgttagcttt
    25801 tactaactta ctacccgtgg ttaattcgcc gagaataccg tatttcgccg gtgaataggc
    25861 tgcagcacca acacccacca gcgtatagcc gagaaacgga ttgataccaa agcagatact
    25921 ggctgcgccc agcagcttca ggccgttggc aaacatcatc acccggcctt tggcgaagct
    25981 atccgccacc tgcccgacaa acggcgccaa aagaatgtaa gcacctacaa acaccatttg
    26041 caggatgggc tggctccact ccggatagaa ctgcgctttc agtaacgcca gagtggcaaa
    26101 cagtagcgcg ttatcgccaa acgcagagag aaactgcgcc acgataaccg ctttcatccc
    26161 cttcgaccac aacgaagtgt tagtgtgcac tgactcactc atcgtgttgt tccgcttcgt
    26221 ctacccagct tttcaacgtg acaaagtcag gtttgccgct gccaagtaat ggcatctgtt
    26281 tcagatagcg aatatcgcgc ggtacagcaa gctccggcac gccgtgctcg cgggcatact
    26341 gttgcaactt atcgcgcgtc agttcgttat ctgtggtgaa aagcaccagt gcctcgcctt
    26401 tgctggcatc gctcttaatc gcagtggcat ggactttatc tggcgaaaca ccaagtgcca
    26461 gttgttccac catttccagc gacaccattt cgcctgcaat tttggcaaag cgttttgcgc
    26521 ggccctgaat ctgcacaaag ccctgctcgt caaaacgcac aatatcgcca gtgtcatacc
    26581 agccgcgctc catttcgccg cgaacattct cggcggtggg cacttccagt acacctggct
    26641 tctccacccg cagatagccg ttcattatgt tcggcccttt cagttgcagg cgtccgccct
    26701 cttcgatacc agggaccgac aacaggcgtg catccattcc tggtagaata cgccctaccg
    26761 taccgggttt cgccgccatc ggtacgttga tagaaacgac aggcgcgcat tcggtcacac
    26821 cgtagccttc aaggatgcgc aggccaaatt tatcctgcca aagctgtttg gtactttctt
    26881 gtagtttttc tgcgcctgcc accacatagc gtagacgata gaagtcatac gggttggcga
    26941 aacgcgcgta gtgaccgagg aaagtcgagg taccgaacaa cacggtgcaa ctgcggtcat
    27001 acaccaagtc cggcacaatg cggtaatgca gcgggcttgg ataaaggaac acttctgcac
    27061 ctgtaagcag tggcgtaaac aggcctaccg tcagcccaaa ggagtgaaac agcggtaatg
    27121 ccgacataaa gcgatcgttg gtggtgaagt cggcaatcgt tttaatctgc tcgacgttcg
    27181 ccagaatgct tttatggcta tggacgacgc cttttggatg gccttcagaa ccggaggtaa
    27241 agaggatcag cgcctcttct tccggctgct gttttacctg cgccaggcgc ggcatcagca
    27301 aatgagcgaa gatccatact ttgtcggcag tggtgacatc tgcttttaaa tcttccagat
    27361 agacccagcg cacctgagta agttgctccg gcagatgcca gagtttgcct ttatcgagaa
    27421 actggcggga agtgaagatg gttttgattt cagccgccgt aatagcactg gtcagccctt
    27481 ttaccccggc agtgtagttc atcattgcgg gaatgcggcg acgcgcgatg gccccaaaaa
    27541 tcactgccgc actgatgcct gcattgggca gcattaagcc gatgcgttcg ccttcaacac
    27601 tgtatttttc aaggatgcgt ccaacaaaca gcgttttcgt aagcaatttg cgataggagt
    27661 ctggggtaaa gttgacgtct tcgacacatt tcttcccggc tccgaagcgg tacattgcac
    27721 tcagtaaaga ttcgtacaga gtttcacgcg ggcgcaccgc catacgcgct tccatcatta
    27781 tttgatgcag catttcgcca gcgattttgc gacggtcacg ggcacgcggc gcatccggca
    27841 tcgccacctg cgttggaggc aaaatatgca gagtaatttg cgggaacaag cgacgtttaa
    27901 ccagaccttt caggcggctg aagtgcgtaa gttccgcccc ttcaatacgc acaggaataa
    27961 ccgttgcacc agacttcgcc gcgacaaaac ccgcgccatc gtagattttc atcagcgagc
    28021 ctgtagtggt gatgcgtcct tcagggaaaa tcaccactgg tcggccctgt tccaccagac
    28081 gtaccagatg tttaatagcc ataggttgcg tcgggtcgag aggaacaaag tcgataaatg
    28141 atttcagcca acgcatatac cactgttggc ttattgaggt gtaaacggca aacactggac
    28201 gcacaggtaa aaacagtcca agcaaaatgc catcaataaa agagacgtga ttaggcgtaa
    28261 ttagtacgcg ctcgcccttc agtgcctggg tgtcacccgt aacgcgaacg cgatacaaaa
    28321 cacggcacaa atttcgaaaa aagctaaaaa gcattccaac tccctttgct ctgattcagt
    28381 aaaagcgaat ggagggagat tacacgagat aaagaacgcg agcgacagta aattaggtgc
    28441 gaaaaaaaac ctgcgcatcc gcgcaggttg gtgcaagaga cagggtacga agagcgtacc
    28501 gaataatctc accaatcaat acctctggga tcttgattgt ggtctgcacg acgactcttc
    28561 gccagcgaga aaacgcaaag gaatgaaggg aaatgcaacg aggtgtgtaa attgtcggtt
    28621 actgttacag attgatgacc ggcaaaaaaa aacctgcgca tctgcgcagg ctggtgtaat
    28681 tcatgtgctc aacacgaagt tgacttcacc tatcaatacc tctgggatca ccactttagc
    28741 aacctgaagc caaacgccac cagcggtcaa tcgcaacagc gtttcgcaaa gtgtaaccaa
    28801 aggtttgcaa tctctttttt tgtcttactt atttgcgttt ggctcacatt cccacgatga
    28861 aaacacgcca ccccttgaac caacgggcgt tttccgtaac actgaaacaa tgtaagcgtt
    28921 tacccactaa ggtattttca tggcgaccat aaaggatgta gcccgactgg caggcgtttc
    28981 agtcgccacc gtttcccgcg tcattaataa ttcacccaaa gccagcgaag cttcccggct
    29041 ggctgtgcat agtgcaatgg agtctcttag ctatcacccg aacgccaacg cccgtgcgct
    29101 ggcgcagcag accactgaaa cggtcggtct gatcgttggt gatgtttccg atccgttttt
    29161 cggtgcaatg gtgaaagcgg tcgaacaggt ggcttatcac accggtaatt tcttattgat
    29221 tggcaacggt taccacaacg aacaaaaaga gcgtcaggct attgagcaac tgatccgcca
    29281 tcgctgtgct gcgttggtcg tccatgccaa aatgatccca gatgccgatt tagcctcatt
    29341 aatgaaacaa atgcccggta tggtgctgat caaccgtatc ctgcctggct ttgaaaaccg
    29401 ttgtattgct cttgacgatc gttacggtgc ctggctggca acgcgtcatt taattcagca
    29461 aggtcatacc cgcattggtt atctgtgctc taaccactct atttctgacg ccgaagatcg
    29521 tctgcaaggg tattacgatg cccttgctga aagtggtatt ccggccaatg atcggctggt
    29581 gacatttggc gaaccagacg aaagcggcgg cgaacaggca atgaccgagc ttttgggacg
    29641 aggaagaaat ttcactgcgg tagcctgtta taacgattca atggcggcgg gcgcgatggg
    29701 cgttctcaat gataatggta ttgatgtacc gggtgagatt tcgttaattg gctttgatga
    29761 tgtgctggtg tcacgctatg tgcgtccgcg cctgaccacc gtgcgttacc caatcgtaac
    29821 gatggcgacc caggctgccg aactggcttt ggcactggcg gataatcgcc ctctcccgga
    29881 aattactaat gtatttagtc cgacgctggt acgtcgtcat tcagtgtcaa ctccgtcgct
    29941 ggaggcaagt catcatgcaa ccagcgacta accgcagtta aagcaattcc agcgccagta
    30001 attcttcgat ggtctggcga cggcgaatca accgcgcctg accattatca aacagaactt
    30061 ctggtaacag cggacggcta ttgtagttgg atgacattga tgcgccatat gcccctgtat
    30121 catgcagtac cagataatca cctgccttca cttccggcaa ggcgcgggtt tcaacatttc
    30181 ccccttcctg ctgggtaaag acatcgcccg attcacataa cggcccggcg acgacggttt
    30241 ccaccgttgg cgcgtgttcc agagaacgac catcagctgc cagggcactg atatggtggt
    30301 aactaccgta cattgccggg cgcatcagat cgttgaaccc ggcatcaacc agcacaaagt
    30361 ggcggctacc catttgtttg acgctccgca cctgagtaat taatacgcca gactgcgcta
    30421 ccaggaagcg acccggttca atttccagtt tcacagggtg gcccaaatgg cgagcgattt
    30481 gctcacgcgc ggcattccac agaccataat aatgttcggt atcaaccgcc tcttcaccct
    30541 gttgataagg aatagaaagc ccaccgcccg cagaaatagc ctgtaaatcc tgaccgaatt
    30601 cgataacctg acgcaccata gcaccacaca cctgctccag atgggcataa tcaacgccag
    30661 aaccaatgtg catgtgaatg ccgactagct gcagatgatg acgttgtatc acgtccagtg
    30721 cggcgggcag atcggtgtac cagataccgt gcttgctgtt ttcgccaccg gtattggttt
    30781 tttggctatg tccgtgacca aaccccggat taacgcgcag ccatacccga tgccctggcg
    30841 aaacctggcc cagttggtcg agcgtatcaa cagaacccgc attcaccgga atttgcaatt
    30901 cactgacgcg ttcaagcgtc gcctgatcga taacatctgc cgtaaaaaca atatcatcgg
    30961 ggtgcgtttg cggattgtaa cccgccgcca acgcacgctc tatttcgcct aacgagacgg
    31021 aatccacttt cacgccctgc tcacgcatta agcgcaaaat atgaatattg gaacaggctt
    31081 tctgtgcaaa gcgcaccact tcaaactgtt tcagcgctgc aatctgccga cgaataattt
    31141 gcgcatcgta gacccacacc gggcagccaa attcagcggg caaacgcagc agattttcgg
    31201 cggtgagatc ggtatcggtg ctgaacagtg aatgtggcat aacaaactcc agatgagtgc
    31261 ttttttatga ttacgccaca tcataaaaag aataaaaaat atcgatttat gtcgagtcta
    31321 tgcaaaaatg atatggatta ccggattgcg agagagcgct aatggccgcc gttaacttac
    31381 gtcatattga aatttttcat gcggtaatga ccgccggaag cctgactgag gcagcacacc
    31441 tgctacacac ctcacagcca accgtcagcc gcgaactggc gcgctttgag aaggtgatcg
    31501 ggctgaaatt gtttgagcgc gtacgtggac gattacatcc taccgtgcaa ggactgcgtc
    31561 tgtttgaaga agtgcaacga tcctggtacg gactggatcg cattgtcagc gccgcagaaa
    31621 gtctgcgcga gtttcgccag ggagaactgt ctattgcctg cctgccggtc ttttcgcaat
    31681 cttttttacc gcagctcctg caaccctttc tggcacgtta tcccgatgtc agcttaaata
    31741 tcgtgcccca ggaatcaccg ctacttgaag agtggctctc ggcccagcgt catgatttag
    31801 gactcactga gacactccat acgcctacgg gaacagaacg taccgaatta ctctctttag
    31861 atgaagtgtg tgtgttacct ccgggtcatc cgctggcggt aaaaaaggta ttaacgccgg
    31921 atgattttca gggtgagaat tacatcagcc tttcccgtac tgacagctat cgccagttgc
    31981 tggatcagct atttactgaa catcaggtta aacgacgcat gatcgtagaa acccacagcg
    32041 ccgcgtcagt ctgcgcaatg gtacgggcgg gggtaggtat ttcggtggtt aacccgctca
    32101 ccgcactaga ttatgcggca agcggtttag tggtgcggcg gttcagtatt gcggttccgt
    32161 tcaccgtcag cctgatccgc cccctgcacc gcccgtcatc agcgctggtt caggcgttta
    32221 gtgggcattt acaagcgggg ttaccgaaac tggtcacttc tcttgacgct attttgtcgt
    32281 cagctacgac agcataaaag cgacagcatc ctcggcatgg atcgccgcgg tatcaaacac
    32341 aggcaaaaca ctgcgctctt ctggcaccag taaaccaatt tctgtgcagc caaaaatgac
    32401 gccctgtgcg ccctgttctg caaggcgagc aatcacttgc gcataataag cgcgtgacgc
    32461 ttcggtaaat tgccccagac acagttcttc aaaaataatc tgattaattt tcgcccgttc
    32521 atccgcttca ggaataagac aattgatgga aaattgttcc gtcagccgcc cgcgataaaa
    32581 atcctgttcc atggtgtaac gcgtacccag cagcgccaca cgagtcattc cggccccggt
    32641 aattgcacgt ccggtggcat ccgcaatgtg taagaaaggc agagtgcaac gtgactcaat
    32701 ggcatccgcc actttatgca tcgtattggt acataacaca ataccttctg cgcccgcccg
    32761 ctgtaagcca agcgccgcct cagccagaat gtccccggtt ttatcccatt ccccgcgacg
    32821 ctggcactct tctatttcat gaaaatcgac gctatgtagc agcatttgcg cagagtgaag
    32881 cccaccaagc cgctgtttaa tgccttcatt tatcagacga tagtaaggaa tggtggattc
    32941 ccagctcatt cctcccagca aaccaattgt tttcattcct ctctcctgat gtgtgttacc
    33001 gcaacaaggc tgaataagga acgagacaaa cgcctcaacg gccaggtgcc caatctctat
    33061 taacgaaaaa agggccggat gtacagcaca tccggcccgt gaaatcagac gccgatattt
    33121 ctcaacttct cgcctgccat cagtttgcgt tcgatatgtt ccagcgtgac atttttggtt
    33181 tccggaatga gccagaaagt aatacccaca aacgcaatgt tcagcgcagt gtagagccag
    33241 aacgtaccgg cagcgccaat gctatcaagc agtgtcagga aggccgcgcc gataatcata
    33301 ttcgacaccc agtttgtcgt cgtcgaacag gtaataccga aatcgcggca tttcagcggc
    33361 tgaatttcag agcacaggat ccacaccact ggcgcggcgc tcatcgcata accggcaata
    33421 cacatcatcg tcatgccaac agagagccag gacaagccac tggaagccgt accgttatca
    33481 aactgcatca ggcaatagcc cagcaccaga gtgcctaacg ccatcacgct gaaaccaatt
    33541 ttcagagccg gtttacgacc tgctttatct accgtaaaga ccgcaatgaa ggtcgcgaac
    33601 ataaaggtca gtcccacgac cagagtcgca atcatctgtt gttctgtggt cgtaaagccc
    33661 gccattttga agatacgcgg cgcgtagtac atgatgatgt tcataccggt aaactgctgc
    33721 atcgcctgca acaacatacc gagaaacaca gcacgacgga cgttacggtt gatcttaaac
    33781 agtgcccaac cgccctgttt taacttcagg ctttcacgaa tttcgttgag ttcttctcgc
    33841 gctttttccg acgtatcgcg cagcatacgc aacacttctt ccgcctcaat atgacgcccc
    33901 ttttccgcca gccagcgcgg gctatttggc aggaagacca ccagaataat cagcagaact
    33961 gctggtaaag caagaacccc caacattgcg cgccagttac cgctataact gaacgctgta
    34021 tcggataaaa acgccagcac gatgccgagt gtgaccatca actggtacat actgatcatc
    34081 ttaccgcgaa cgttttcact tgccatttca gaaagataca gaggagcggt gtaagacgcg
    34141 atcccgacag caatgcccag caccacacga gcggcgatta acatctctac gctggtcgca
    34201 aaagcggacc ctatagaacc gagtacaaac aggatggccc ccgccatcag gctgtattta
    34261 cgccccaggc ggaacgacag ccaaccatta aacagcgcac caattgctgc gccgagcatc
    34321 atgctactaa ccacccattc ctgcaaacga ctggtcagca caaagtgatc ggtaatgaac
    34381 ggcaacgctc cggcgattac gccgatatca agaccaaata acaatcctgc gaccgcagca
    34441 gctaccgaaa caaacatatt catacgccgc gtatcacgca aagaacgtgg cgttaaagca
    34501 gattccgtat tgatagtaac cattttttcc tgccagcaga gagtaagaca ttgtgaaaaa
    34561 atacgtgaac aattcacaca ggtgtcaggt cggaaacagc ataaatatgg attaaattgc
    34621 tgcgacatgc cgttatgtga tggatattcc aattttaaaa ttaagttgaa ttattgagat
    34681 tattattaac ctcctaattt taaagcagat aaaattcata aagttcatta attgataatt
    34741 gatatggatt atttcataac catgatatgg attatgatga tctagagata taaaaaaccc
    34801 tgccatgcgg cagggtcata aaagtaagag gaatgaatta acgcgccagc caaccgccat
    34861 ccacagcaat ggtataacca tttacataat ctgaagcgct ggaggcaaga aacactaccg
    34921 gccccatcag gtcactcggc agtccccaac ggccagctgg aatgcgatcg agaatttccg
    34981 cgctacgttg ttcatctgcc cgcagttgtt gggtattgtt ggtcgccatg taacccggag
    35041 ctatcgcatt aacattaatg ttgtgtttag cccattcgtt tgccatcaat cgcgtcacac
    35101 ccatcacgcc gctttttgat gcggtataag aaggcacacg gatcccgccc tggaaggaga
    35161 gcattgacgc gatattgata atcttgccgc cattgccttg cgcgataaag tgtttcgccg
    35221 ctgcctgaga catgaagaat acgctcttga tattcaggtt catgacatcg tcccagtctt
    35281 tttcgctgaa ctcgagagcg tcttcgcggc gaatcaatcc ggcgttattc accaggatat
    35341 caatatgacc aaattcagct accgcgcgat ccagcagtcc aggaatgcca tcaatctttc
    35401 gcagatcggc ggtcaggctt aaaaaacgac gccccagcgc cgtgacctgc tcgatggttt
    35461 cagtcggttc aacgatgtta atgccaacaa tgtcacagcc cgcttgcgcc agccccaacg
    35521 ccatcccctg gcccagccca gtatcacaac cagtgacgac cgcaacttta ccttggagag
    35581 aaaatgcact taaaatcata acaacacctt attcgtttat gcccacaact agcgcaaatc
    35641 tttaacgccc acatggtcca tatcattaaa gacctggttt tcaccgacca tgccccagat
    35701 aaaggtataa gctttggttc cgacgccgga atggatcgac cagctcgggg agatcaccgc
    35761 ctgctcgtta tgcatcacaa tgtgacgcgt ttcttgcggc tgccccatca tgtggaaaac
    35821 gcaggcgtca tcatccatat tgaaatagaa ataaacttcc atccggcgct cgtgggtgtg
    35881 acacggcatg gtgttccaca agttacctgg agccagctcc gtcagcccca tactcaattg
    35941 acaggtttcc agtacatccg ggacaaaata tttgttaatc gtgcgacggt tactggtgag
    36001 gttatcgcct aacgtgactg gagatacttc gtccggtgtg acttttttgg tgggatacgt
    36061 cgtatgcgcg ggtgcgcaat tgtaataaaa cttcgccgga gtggcggtat caatactggc
    36121 aaagacaact tcttttgcac ctttaccaac atacagggcg tcgcggtgac cgatttcata
    36181 gcattgacca tcgacagtaa tcgtaccggc accgccaata ttgataacac ctaactcgcg
    36241 acgttcaagg aaatagctta cgccgagttg tttaccaact tccccgccaa cggaaaccgt
    36301 tttagttatc ggcataatgc cgccaacaat aattcggtca atgtggctgt aaaccatggt
    36361 gtactcatcg gcgacaaata ccttttcaac caaaaattca ttgcgcagcc cttgggtatc
    36421 cagcgttttt gcgtgcgcac cgtggatgct ctgtcttacg tccacatcaa cctccgataa
    36481 tcagtgaagc gtgaaaatag aaaacgatag tgaaacactg tttcgttttc tctataaagg
    36541 gatgatatgc ccatgggcta gggttttcaa ttaaaaataa aacaatgttt tatttttaat
    36601 ttaaggatta aaaaaagtgc agtgtcgatc acgaatatgt catgagaatg gaaaaatgag
    36661 ggagcccaga aaatgtatgt cgacttgcca gccatcatta ttcaacagat taatgacggc
    36721 agatatttat cggtaaggag gaaatgcaga gctactcgtc acgttcaatg gtcaatgcca
    36781 cgccctggcc cccgccgata caaagcgttg ccagcccttt gcgggcatta cgtttcacca
    36841 tttcatgaac cagagaaacc aggattcggc aaccggaagc gcctatcggg tgaccgagtg
    36901 cgatcgcgcc accattgaca ttgacccgac gctcatccca ctcaagcatc ttgccaaccg
    36961 aaagcgcctg tgcagcaaac gcttcattag cctcgataag atcgacatca gccaactgcc
    37021 agcctacacg ctccaggcaa cggcgggtcg catacaccgg cgcaattccc atcaatgccg
    37081 gatctacacc aacgctggca aatgcgcgga tgcgggccag cacgggtaaa ttcaacgctc
    37141 gtgctttggc ttcgctcatc atcattaccg cagctgcgcc atcgtttatg gatgatgcat
    37201 tccccgcggt cacagaaccg agactatcaa atgaaggatt taaacgggct aaaccttctg
    37261 cactggcgtc agtgcgtggc tgttcatcgg tatcaacaac caacgtctga ccgttacttt
    37321 ggatcattac cgggacgatc tcatctttaa atcgtccggc gtcaatcgcc gctcgcgctt
    37381 tttgttgcga gctaagtgcg taagcatcct gcaactgacg gctgatgcca tattcgcgag
    37441 ccagattttc ggcggtgaca ccaatatgat aatcattgaa ggcatcccac aacccatcat
    37501 gcaccagact gtcaaccaac tggctattgc caagctgttc accggtgcgg ctatcagtca
    37561 gaacatgtgg tgcgcggctc atgttttcct ggccacctgc gatgacaata tcagcctcgc
    37621 cacactgtat cgcctgagta gccagatgca gtgctttaag accggaaccg caaacgtcat
    37681 taatcgtgat tgcagaaacg ctgttaggaa gaccaccttt aatagccgat tgccttgccg
    37741 gattctgccc tgcccctgca gtcaacacct gaccaagaat tacttcatcc accgcatatg
    37801 caggaacgcc ggtacgttct attaacgctt tcacgaccag actaccaagt tccacggcgg
    37861 aatgacccgc taacgcacca cgaaagcagc cgataggtgt ccgtaacgcc ccgacaatca
    37921 caacgtcttt catcacaacc tcgcaccaat taacagtgca atagtaaatg attgttaatt
    37981 acagttatct taattgttta aaaaaagtga tttttatcac aaaggaaata tgcctgagca
    38041 gtagtcagag tcataactgg cacgtaaggt ttgcaaccac taacccacca atagatgggt
    38101 agatagggcg ttaatctccc atacttaact tggtttatgg taaattgccc tccattttgt
    38161 ttaatttgta gatgatacgt tcagataatg tctaatattt ggtctaaaga agaaactctg
    38221 tgaagtttcg cgctctatgg cacagccgtt gatgcaggca cgctattcct tcctattcag
    38281 ttaggttcgg caggggctgt ggtcctgttt attactgctc tggtcgcctg gcctttaaca
    38341 tattggccac ataaagcctt atgccagttc atcctctcat caaaaacatc agcaggtgaa
    38401 gggataacgg gcgcggtaac acactactat ggcaagaaga ttggtaatct gattaccacg
    38461 ctgtacttca tcgccttttt tgtcgtcgtg ttgatatatg cagtggcaat taccaactca
    38521 cttacggaac agctggcaaa gcatatggtt attgatcttc gcatccgtat gttggtgagt
    38581 ctgggtgttg tattaattct gaatctcatt tttctgatgg gacgtcatgc cactattcgg
    38641 gtaatgggat ttttggtatt cccattgatt gcctatttct tatttctttc catttacctt
    38701 gtcggtagtt ggcaacctga tctattaaca acccaggtag agttcaatca gaataccctt
    38761 caccagatat ggatatcgat tcccgtgatg gttttcgcct ttagccatac gcccattatt
    38821 tctacgtttg ccatagacag acgtgaaaaa tatggcgaac acgctatgga taaatgcaaa
    38881 aaaattatga aagtcgctta tctcatcatc tgcataagtg tactgttctt tgtctttagc
    38941 tgcctgcttt ctattccatc ttcgtatatt gaagcggcta aagaagaagg ggtcaccatt
    39001 ttatcggcgc tttctatgct gccgaacgcc ccagcatggt tgtcaatttc cgggattatt
    39061 gtcgcagtag ttgcgatgtc gaaatcattc ctgggtacgt actttggcgt tattgaaggt
    39121 gccacagagg tcgtcaaaac aacactacag caggttggtg taaagaaaag tcgtgcattt
    39181 aaccacgcac tatcaattat gttggtatcg ctgattacct tcattgtttg ttgcattaac
    39241 ccgaacgcga tttcgatgat ttacgcgatc agcggcccgc tcattgccat gatacttttc
    39301 atcatgccta cgctgtcaac gtatctcatc ccggcgctta aaccctggcg ttccatcgga
    39361 aatctgatta cgctgatcgt gggtatcctg tgcgtatcgg taatgttctt tagctaatat
    39421 ccgttgtgga tggttcaaaa ttgctacaat tttgaaccat tccatttttc ataatattca
    39481 tttgttatta ttccgcccta tcacttctct cgtcatatcc tagaattatc agttagttaa
    39541 acacacaaag agatctgaac atcttaaata agactattta agatgcataa cttagattcg
    39601 caagatatct tctggtcatt caaaaacaat ttgcgatata gatattctca taccatgaga
    39661 tagttatctg gaccacgttc gaccatgcaa gattaaatca gtgaaacata atcatattta
    39721 ataacgagaa atgcattttt aaatatctta atcagaatta tagcaaatac agattaatcg
    39781 caatttttcc cagcaaaatt acaaaatcaa ggtgtattga catcatcaat caccaatgaa
    39841 attatgcaat tatatacgga tagggaggtt cttaacatgg ggctttgtag tcgttataaa
    39901 agtcttacat gcaatagttg ctcaatgcat tgccaaataa tgccagaaga gtcaccgcgt
    39961 ttgcagtact gcgcaaactc gtgtt
//
LOCUS       DAJTRC010000027        39882 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.027, whole genome shotgun sequence.
ACCESSION   DAJTRC010000027 DAJTRC010000000
VERSION     DAJTRC010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 39882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 39882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..39882
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.027"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..292)
                     /locus_tag="OO357_001525"
     CDS             complement(<1..292)
                     /locus_tag="OO357_001525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5886516.1"
                     /translation="MTQYSSLLRGLAAGSAFLFLFAPTAFAAEQTVEAPSVDARAWIL
                     MDYASGKVLAEGNADEKLDPASLTKIMTSYVVGQALKADKIKLTDMVTVGKDA"
     gene            538..1164
                     /gene="gstB"
                     /locus_tag="OO357_001526"
     CDS             538..1164
                     /gene="gstB"
                     /locus_tag="OO357_001526"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308945.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase GstB"
                     /protein_id="HCR5886517.1"
                     /translation="MITLWGRNNSTNVKKVLLTLEELELPYEQILAGREFGINHDADF
                     LAMNPNGLVPLLRDDESDLILWESNAIVRYLAAQYGQKRLWIDSPARRAEAEKWMDWA
                     NQTLSNAHRGILMGLVRTPPEERDQAAIDASCKECDALFALLDAELAKVKWFSGDEFG
                     VGDIAIAPFIYNLFNVGLTWTPRPNLQRWYQQLTERPAVRKVVMIPVS"
     gene            complement(1161..2275)
                     /gene="yliI"
                     /locus_tag="OO357_001527"
                     /pseudo
     CDS             complement(1161..2275)
                     /gene="yliI"
                     /locus_tag="OO357_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415358.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldose sugar dehydrogenase YliI"
     gene            complement(2386..2769)
                     /gene="bssR"
                     /locus_tag="OO357_001528"
     CDS             complement(2386..2769)
                     /gene="bssR"
                     /locus_tag="OO357_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator BssR"
                     /protein_id="HCR5886518.1"
                     /translation="MFVDRQRIDLLNRLIDARVDLAAYVQLRKAKGYMSVSESNHLRD
                     NFFKLNRELHDKSLRLNLHLDQEEWSALHHAEEALATAAVCLMSGHHDCPTVITVNAD
                     KLENCLMSLTLSIQSLQKHAMLEKA"
     gene            2982..4307
                     /gene="rimO"
                     /locus_tag="OO357_001529"
     CDS             2982..4307
                     /gene="rimO"
                     /locus_tag="OO357_001529"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HCR5886519.1"
                     /translation="MSKVTPQPKIGFVSLGCPKNLVDSERILTELRTEGYDVVPSYDD
                     ANMVIVNTCGFIDSAVQESLEAIGEALNENGKVIVTGCLGAKEDQIREVHPKVLEITG
                     PHSYEQVLEHVHHYVPKPKHNPFLSLVPEQGVKLTPRHYAYLKISEGCNHRCTFCIIP
                     SMRGDLMSRPIGEVLSEAKRLVDAGVKEILVISQDTSAYGVDVKHRTGFHNGEPVKTS
                     MVSLCEQLSKLGIWTRLHYVYPYPHVDDVIPLMAEGKILPYLDIPLQHASPRILKLMK
                     RPGSVDRQLARIKQWREICPELTLRSTFIVGFPGETEEDFQMLLDFLKEARLDRVGCF
                     KYSPVEGADANALPDQVPEEVKEERWNRFMQLQQQISAERLQEKVGREILVIIDEVDE
                     EGAIGRSMADAPEIDGAVYLNGETNVKPGDILRVKVEHADEYDLWGSRV"
     gene            complement(4354..5682)
                     /locus_tag="OO357_001530"
     CDS             complement(4354..5682)
                     /locus_tag="OO357_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5886520.1"
                     /translation="MSRINKFVLTVSLLIFIMISAVACGIYTQMVKERVYSLKQSVID
                     TAFAVANIAEYRRSVAIDLINTLNPTEEQLLVGLRTAYADSVSPSYLYDVGPYLISSD
                     ECIQVKEFEKNYCADIMQVVKYRHVKNTGFISFDGKTFVYYLYPVTHNRSLIFLLGLE
                     RFSLLSKSLVMDSENLMFSLFKNGKPVTGDEYNAKNAIFTVSEAMEHFAYLPTGLYVF
                     AYKKDVYLRVCTLIIFFAALVAVILGASCLYLVRRVINRGIVEKEAIINNHFERVLDG
                     GLFFSAADVKKLYSMYNSAFLDDLTKAMGRKSFDEDLKALPEKGGYLCLFDVDKFKNI
                     NDTFGHLLGDEVLMKVVKILKSQIPVDKGKVYRFGGDEFAVIYTGGTLEELLSILKEI
                     VHFQVGSINLSTSIGVAHSNECTTVERLKMLADERLYKSKKNGRAQISWQ"
     gene            complement(5690..8032)
                     /locus_tag="OO357_001531"
                     /pseudo
     CDS             complement(5690..8032)
                     /locus_tag="OO357_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415354.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            complement(8210..9121)
                     /gene="gsiD"
                     /locus_tag="OO357_001532"
     CDS             complement(8210..9121)
                     /gene="gsiD"
                     /locus_tag="OO357_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiD"
                     /protein_id="HCR5886521.1"
                     /translation="MRLFNWRRQAVLNAMPLVKPDQVRTPWHEFWRRFRRQHMAMTAA
                     LFVILLIVVAIFARWIAPYDAENYFDYDNLNNGPSLQHWFGVDSLGRDIFSRVLVGAQ
                     ISLAAGVFAVFIGVAIGTLLGLLAGYYEGWWDRLIMRICDVLFAFPGILLAIAVVAVL
                     GSGIANVIIAVAIFSIPAFARLVRGNTLVLKQQTFIESARSIGASDMTILLRHILPGT
                     VSSIVVFFTMRIGTSIISAASLSFLGLGAQPPTPEWGAMLNEARADMVIAPHVAVFPA
                     LAIFLTVLAFNLLGDGLRDALDPKIKG"
     gene            complement(9124..10044)
                     /gene="gsiC"
                     /locus_tag="OO357_001533"
     CDS             complement(9124..10044)
                     /gene="gsiC"
                     /locus_tag="OO357_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter permease GsiC"
                     /protein_id="HCR5886522.1"
                     /translation="MLYYVIKRLLGLIPTLFIVSVLVFLFVHMLPGDPARLIAGPEAD
                     AQVIELVRQQLGLDQPLYHQFWHYISNAVQGDFGLSMVSRRPVADEIASRFMPTLWLT
                     ITSMVWAVIFGMAAGIIAAVWRNRWPDRLSMTIAVSGISFPAFALGMFLIQVFSVELG
                     WLPTVGADSWQHYILPSLTLGAAVAAVMARFTRASFVDVLSEDYMRTARAKGVSETWV
                     VLKHGLRNAMIPVVTMMGLQFGFLLGGSIVVEKVFNWPGLGRLLVDSVEMRDYPVIQA
                     EILLFSLEFILINLVVDVLYAAINPAIRYK"
     gene            complement(10062..11600)
                     /gene="gsiB"
                     /locus_tag="OO357_001534"
     CDS             complement(10062..11600)
                     /gene="gsiB"
                     /locus_tag="OO357_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter substrate-binding
                     protein GsiB"
                     /protein_id="HCR5886523.1"
                     /translation="MARAVHRSGLVALGIATALMASCAFAAKDVVVAVGSNFTTLDPY
                     DANDTLSQAVAKSFYQGLFGLDKEMKLKNVLAESYTVSDDGITYTVKLREGIKFQDGT
                     DFNAVAVKANLDRASDPANHLKRYNLYKNIAKTEAIDPTTVKITLKQPFSAFINILAH
                     PATAMISPTALEKYGKEIGFHPVGTGPYELDTWNQTDFVKVKKFAGYWQPGLPKLDSI
                     TWRPVADNNTRAAMLQTGEAQFAFPIPYEQATLLEKNKNIELMASPSIMQRYISMNVT
                     QKPFDNPKVREALNYAINRPALVKVAFAGYATPATGVVPPSIAYAQSYKPWPYDPVKA
                     RELLKEAGYPNGFSTTLWSSHNHSTAQKVLQFTQQQLAQVGIKAQVTAMDAGQRAAEV
                     EGKGQKESGVRMFYTGWSASTGEADWALSPLFASQNWPPTLFNTAFYSNKQVDDFLAQ
                     ALKTNDPAEKTRLYKAAQDIIWQESPWIPLVVEKLVSAHSKNLTGFWIMPDTGFSFED
                     ADLQ"
     gene            complement(11620..13491)
                     /gene="gsiA"
                     /locus_tag="OO357_001535"
     CDS             complement(11620..13491)
                     /gene="gsiA"
                     /locus_tag="OO357_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione ABC transporter ATP-binding protein
                     GsiA"
                     /protein_id="HCR5886524.1"
                     /translation="MPHSDELDAGNVLAVENLNIAFMQDQQKIAAVRNLSFSLQRGET
                     LAIVGESGSGKSVTALALMRLLEQAGGLVQCDKMLLRRRSRDVIELSEQSAAQMRHVR
                     GADMAMIFQEPMTSLNPVFTVGEQIAESIRLHQNASREEAMVEAKRMLDQVRIPEAQT
                     ILSRYSHQLSGGMRQRVMIAMALSCRPAVLIADEPTTALDVTIQAQILQLIKVLQKEM
                     SMGVIFITHDMGVVAEIADRVLVMYQGEAVETGTVEQIFHAPQHPYTRALLAAVPQLG
                     AMKGLDYPRRFPLISLEHPAKQEPPIEQKTVVDGEPVLRVRNLVTRFPLRSGLLNRVT
                     REVHAVEKVSFDLWPGETLSLVGESGSGKSTTGRALLRLVESQGGEIIFNGQRIDTLS
                     PGKLQALRRDIQFIFQDPYASLDPRQTIGDSIIEPLRVHGLLPGKDAAARVAWLLERV
                     GLLPEHAWRYPHEFSGGQRQRICIARALALNPKVIIADEAVSALDVSIRGQIINLLLD
                     LQRDFGIAYLFISHDMAVVERISHRVAVMYLGQIVEIGSRCAVFENPQHPYTRKLLAA
                     VPVAEPSRQRPQRVLLSDDLPSNIHLRGEEVAAVSLQCVGPGHYVAQPQSEYAFMRR"
     gene            complement(13478..14443)
                     /gene="iaaA"
                     /locus_tag="OO357_001536"
     CDS             complement(13478..14443)
                     /gene="iaaA"
                     /locus_tag="OO357_001536"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5886525.1"
                     /translation="MGKAVIAIHGGAGAISRAQMSLQQELRYIEALSAIVETGQKMLE
                     AGESALDVVTEAVRLLEECPLFNAGIGAVFTRDETHELDACVMDGNTLKAGAVAGVSH
                     LRNPVLAARLVMEQSPHVMMIGEGAENFAFAHGMERVSPEIFSTPLRYEQLLAAREEG
                     ATVLDHSGAPLDEKQKMGTVGAVALDLDGNLAAATSTGGMTNKLPGRVGDSPLVGAGC
                     YANNASVAVSCTGTGEVFIRALAAYDIAALMDYGGLSLAEACERVVMEKLPALGGSGG
                     LIAIDHEGNVALPFNTEGMYRAWGYAGDTPTTGIYREKGDTVATQ"
     gene            14647..15882
                     /gene="moeA"
                     /locus_tag="OO357_001537"
     CDS             14647..15882
                     /gene="moeA"
                     /locus_tag="OO357_001537"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HCR5886526.1"
                     /translation="MEFTTGLMSLDTALNEMLSRVTPLTTQETLPLVQCFGRILASDV
                     VSPLDVPGFDNSAMDGYAVRLADIASGQPLPVAGKSFAGQPYHGEWPAGTCIRIMTGA
                     PVPEGCEAVVMQEQTEQTDNGVRFTAEVRSGQNIRRRGEDISAGAVVFPAGTRLTTAE
                     LPVIASLGIAEVPVIRKVRVALFSTGDELQLPGQPLGDGQIYDTNRLAVHLMLEQLGC
                     EVINLGIIRDDPHALRAAFIEADSQADVVISSGGVSVGEADYTKMILEELGEIAFWKL
                     AIKPGKPFAFGKLSNSWFCGLPGNPVSATLTFYQLVQPLLAKLSGNTASGLPARQRVR
                     TASRLKKTPGRLDFQRGVLQRNADGELEVTTTGHQGSHIFSSFSLGNCFIVLERDRGN
                     VDVREWVEVEPFNALFGGL"
     gene            15882..16631
                     /gene="moeB"
                     /locus_tag="OO357_001538"
     CDS             15882..16631
                     /gene="moeB"
                     /locus_tag="OO357_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HCR5886527.1"
                     /translation="MAELSDQEMLRYNRQIILRGFDFDGQEALKDSRVLVVGLGGLGC
                     AASQYLASAGVGNLTLLDFDTVSLSNLQRQTLHSDATVGQPKVESARDALTRINPHIA
                     ITPVNALLDDAELAALIAEHDLVLDCTDNVAVRNQLNAGCFAAKVPLVSGAAIRMEGQ
                     ITVFTYQDGEPCYRCLSRLFGENALTCVEAGVMAPLIGVIGSLQAMEAIKLLAGYGKP
                     ASGKIVMYDAMTCQFREMKLMRNPGCEVCGQ"
     gene            complement(16810..17472)
                     /gene="fsa"
                     /locus_tag="OO357_001539"
     CDS             complement(16810..17472)
                     /gene="fsa"
                     /locus_tag="OO357_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
                     /protein_id="HCR5886528.1"
                     /translation="MELYLDTSDVVAVKALSRIFPLAGVTTNPSIIAAGKKPLDVVLP
                     QLHEAMGGQGRLFAQVMATTAEGMVNDALKLRSIIADIVVKVPVTAEGLAAIKMLKAE
                     GIPTLGTAVYGAAQGLLSALAGAEYVAPYVNRIDAQGGSGIQTVTDLHQLLKMHAPQA
                     KVLAASFKTPRQALDCLLAGCESITLPLDVAQQMISYPAVEAAVTKFEQDWQGAFGRT
                     SI"
     gene            17603..18502
                     /locus_tag="OO357_001540"
     CDS             17603..18502
                     /locus_tag="OO357_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HCR5886529.1"
                     /translation="MIFNIQRYSTHDGPGIRTVVFLKGCSLGCRWCQNPESRARTQDL
                     LYDARLCLEGCELCAKAAPEVIERALNGLLIHREKLTPEHLTALTDCCPTQALTVCGE
                     VKSVEEIMATVLRDKPFYDRSGGGLTLSGGEPFMQPEMATALLQACHEAGIHTAVETC
                     LHVPWKYIAPSLPYIDLFLADLKHVADAPFKQWTDGNAARVLDNLKKLAAAGKKIIIR
                     VPLIQGFNADETSVKAITDFAADELHVGEIHFLPYHTLGINKYHLLNLPYDAPEKPLD
                     APELLDFAQQYACQKGLTATLRG"
     gene            18508..20940
                     /locus_tag="OO357_001541"
     CDS             18508..20940
                     /locus_tag="OO357_001541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl radical protein"
                     /protein_id="HCR5886530.1"
                     /translation="MTTLKLDTLSDRIKAHKNALVHIVKPPVCTERAQHYTEMYQQHL
                     DKPIPVRRALALAHHLANRTIWIKHDELIIGNQASEVRAAPIFPEYTVSWIEKEIDDL
                     ADRPGAGFAVSEENKRVLHEVCPWWRGQTVQDRCYGMFTDEQKGLLATGIIKAEGNMT
                     SGDAHLAVNFPLLLEKGLDGLREKVAERRSRINLTVLEDLHGEQFLKAIDIMLVAVSE
                     HIERFAALARAMAATETRESRRDELLAMAENCDLIAHQPPQTFWQALQLCYFIQLILQ
                     IESNGHSVSFGRMDQYLYPYYRRDVELNQTLDREHAIELLHSCWLKLLEVNKIRSGSH
                     SKASAGSPLYQNVTIGGQNLVDGQPMDAVNPLSYAILESCGRLRSTQPNLSVRYHAGM
                     SNDFLDACVHVIRCGFGMPAFNNDEIVIPEFIKLGIEPQDAYDYAAIGCIETAVGGKW
                     GYRCTGMSFINFARVMLAALEGGRDATSGKVFLPQEKALSAGNFNNFDEVMDAWDTQI
                     RYYTRKSIEIEYVVDTMLEENVHDILCSALVDDCIERAKSIKQGGAKYDWVSGLQVGI
                     ANLGNSLAAVKKLVFEQGAIGQQQLAAALADDFDGLTHEQLRQRLINGAPKYGNDDDT
                     VDTLLARAYQTYIDELKQYHNPRYGRGPVGGNYYAGTSSISANVPFGAQTMATPDGRK
                     AHTPLAEGASPASGTDHLGPTAVIGSVGKLPTAAILGGVLLNQKLNPATLENESDKQK
                     LMILLRTFFEVHKGWHIQYNIVSRETLLEAKKHPDQYRDLVVRVAGYSAFFTALSPDA
                     QDDIIARTEHML"
     gene            21086..21901
                     /gene="ybiV"
                     /locus_tag="OO357_001542"
     CDS             21086..21901
                     /gene="ybiV"
                     /locus_tag="OO357_001542"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase YbiV"
                     /protein_id="HCR5886531.1"
                     /translation="MSVKVIVTDMDGTFLNDAKTYNQPRFMAQYQELKKRGIKFVVAS
                     GNQYYQLISFFPELKDEISFVAENGALVYEHGKQLFHGELTRHESRIVIGELLKDKQL
                     NFVACGLQSAYVSENAPEAFVALMAKHYHRLKPVKDYQEIDDVLFKFSLNLPDEQIPL
                     VIDKLHIALDGIMKPVTSGFGFIDLIIPGLHKANGISRLLKRWDLSPQNVVAIGDSGN
                     DAEMLKMARYSFAMGNAAENIKQIARYATDDNNHEGALNVIQAVLDNTSPFNS"
     gene            22053..23318
                     /locus_tag="OO357_001543"
     CDS             22053..23318
                     /locus_tag="OO357_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1479 domain-containing protein"
                     /protein_id="HCR5886532.1"
                     /translation="MASTFTSDTLPADHKAAIRQMKHALRAQLGDVQQIFNQLSDDIA
                     TRVAEINALKAQGDAVWPVLSYADIKAGHVTAEQREQIKRRGCAVIKGHFPREQALGW
                     DQSMLDYLNRNRFDEVYKGPGDNFFGTLSASRPEIYPIYWSQAQMQARQSEEMANAQS
                     FLNRLWTFESDGKQWFNPDVSVIYPDRIRRLPPGTTSKGLGAHTDSGALERWLLPAYQ
                     HVFANVFNGNLAKYDPWHAAHRTEVEEYTVDNTTKCSVFRTFQGWTALSDMLPGQGLL
                     HVVPIPEAMAYVLLRPLLDDVPEDELCGVAPGRVLPVSEQWHPLLIEALTSIPKLEAG
                     DSVWWHCDVIHSVAPVENQQGWGNVMYIPAAPMCEKNLAYAHKVKAALEKGASPGDFP
                     REDYETNWEGRFTLADLNIHGKRALGMDV"
     gene            complement(23559..25151)
                     /locus_tag="OO357_001544"
     CDS             complement(23559..25151)
                     /locus_tag="OO357_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATPase"
                     /protein_id="HCR5886533.1"
                     /translation="MLVSSNVTMQFGSKPLFENISVKFGGGNRYGLIGANGSGKSTFM
                     KILGGDLEPTLGNVSLDPNERIGKLRQDQFAFEEFTVLDTVIMGHKELWEVKQERDRI
                     YALPEMSEEDGYKVADLEVKYGEMDGYSAEARAGELLLGVGIPVEQHYGPMSEVAPGW
                     KLRVLLAQALFAEPDILLLDEPTNNLDIDTIRWLEQVLNERDSTMIIISHDRHFLNMV
                     CTHMADLDYGELRVYPGNYDEYMTAATQARERLLADNAKKKAQIAELQSFVSRFSANA
                     SKSRQATSRARQIDKIKLEEVKASSRQNPFIRFEQDKKLFRNALEVEGLTKGFDNGPL
                     FKNLNLLLEVGEKLAVLGTNGVGKSTLLKTLVGDLQPDSGTVKWSENARIGYYAQDHE
                     YEFENDLTVFEWMSQWKQEGDDEQAVRSILGRLLFSQDDIKKPAKVLSGGEKGRMLFG
                     KLMMQKPNILIMDEPTNHLDMESIESLNMALELYQGTLIFVSHDREFVSSLATRILEI
                     TPERVIDFSGNYEDYLRSKGIE"
     gene            25370..26290
                     /gene="ldtB"
                     /locus_tag="OO357_001545"
     CDS             25370..26290
                     /gene="ldtB"
                     /locus_tag="OO357_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5886534.1"
                     /translation="MNMKLKTLFAAAFAVVGFCSTASAVTYPLPTDGSRLVGQNQVIT
                     IPEGNTQPLEYFAAEYQMGLSNMMEANPGVDTFLPKGGTVLNIPQQLILPDTVHEGIV
                     INSAEMRLYYYPKGTNTVIVLPIGIGQLGKDTPINWTTKVEHKKAGPTWTPTAKMHAE
                     YRAAGEPLPAVVPAGPDNPMGLYALYIGRLYAIHGTNANFGIGLRVSHGCVRLRNEDI
                     KFLFEKVPVGTRVQFIDEPVKATTEPDGSRYIEVHNPLSTTEAQFEGQEIVPITLTKS
                     VQTVTGQPDVDQVVLDEAIKNRSGMPVRLN"
     gene            complement(26348..27466)
                     /locus_tag="OO357_001546"
     CDS             complement(26348..27466)
                     /locus_tag="OO357_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion transporter"
                     /protein_id="HCR5886535.1"
                     /translation="MSLPFLRTLQGDRFFQLLILVGIGLSFFVPFAPKSWPAAIDWHT
                     IITLSGLMLLTKGVELSGYFDVLGRKMVRRFATERRLAMFMVLAAALLSTFLTNDVAL
                     FIVVPLTITLKRLCEIPVNRLIIFEALAVNAGSLLTPIGNPQNILIWGRSGLSFAGFI
                     AQMAPMAGAMMLTLLLLCWCCFPGKALQYHTGVQTPEWKPRLVWNCLGLYIVFLTALE
                     FKQELWGLVIVAAGFVLLARRVVLSVDWTLLLVFMAMFIDVHLLTQLPALQGVLGNVS
                     HLSEPGLWLTAVGLSQVISNVPSTILLLNYVPPSLLLAWAVNVGGFGLLPGSLANLIA
                     LRMANDRRIWWRFHLYSIPMLLWAALVGYVLLVMIPAW"
     gene            complement(27463..27930)
                     /gene="mntR"
                     /locus_tag="OO357_001547"
     CDS             complement(27463..27930)
                     /gene="mntR"
                     /locus_tag="OO357_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-binding transcriptional regulator
                     MntR"
                     /protein_id="HCR5886536.1"
                     /translation="MSRRAGTPIAKKVTQLVNVEEHVEGFRQVREAHRRELIDDYVEL
                     ISDLIREVGEARQVDMAARLGVSQPTVAKMLKRLATMGLIEMIPWRGVFLTAEGEKLA
                     QESRERHQIVENFLLVLGVSPEIARRDAEGMEHHVSEETLDAFRLFTQKHGAK"
     gene            28116..28244
                     /gene="mntS"
                     /locus_tag="OO357_001548"
     CDS             28116..28244
                     /gene="mntS"
                     /locus_tag="OO357_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganase accumulation protein MntS"
                     /protein_id="HCR5886537.1"
                     /translation="MNEFKRCMRVFSHSPFKVRLMLLSMLCDMVNNKPQQDKPSDK"
     gene            28516..30099
                     /gene="opgE"
                     /locus_tag="OO357_001549"
     CDS             28516..30099
                     /gene="opgE"
                     /locus_tag="OO357_001549"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase"
                     /protein_id="HCR5886538.1"
                     /translation="MNLTLKESLVTRSRVFSPWTAFYFLQSLLINLGLGYPFSLLYTA
                     AFTAILLLLWRTLPRVQKVLVGVSSLVAACYFPFAQAYGAPNFNTLLALHSTNMEEST
                     EILTIFPWYSYLVGLFIFALGVIAIRRKKENEKARWNTFDSLCLVFSVATFFVAPVQN
                     LAWGGVFKLKDTGYPVFRFAKDVIVNNNEVIEEQERMAKLSGMKDTWTVTAVKPKYQT
                     YVVVIGESARRDALGAFGGHWDNTPFASSVNGLIFADYIAASGSTQKSLGLTLNRVVD
                     GKPQFQDNFVTLANRAGFQTWWFSNQGQIGEYDTAIASIAKRADEVYFLKEGNFEADK
                     NTKDEALLDMTAQVLAQEHSQPQLIVLHLMGSHPQACDRTQGKYETFVQSKETSCYLY
                     TMTQTDDLLRKLYDQLRNSGSSFSLVYFSDHGLAFKERGKDVQYLAHDDKYQQNFQVP
                     FMVISSGDKAHRVIKARRSANDFLGFFSQWTGIKAKEINIKYPFISEKKAGPIYITNF
                     QLQKVDYNHLGTDIFDPKP"
     gene            complement(30147..30662)
                     /gene="ompX"
                     /locus_tag="OO357_001550"
     CDS             complement(30147..30662)
                     /gene="ompX"
                     /locus_tag="OO357_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein OmpX"
                     /protein_id="HCR5886539.1"
                     /translation="MKKIACLSALAAVLAFTAGTSVAATSTVTGGYAQSDAQGQMNKM
                     GGFNLKYRYEEDNSPLGVIGSFTYTEKSRTASSGDYNKNQYYGITAGPAYRINDWASI
                     YGVVGVGYGKFQTTEYPTYKHDTSDYGFSYGAGLQFNPMENVALDFSYEQSRIRSVDV
                     GTWIAGVGYRF"
     gene            complement(30715..30780)
                     /locus_tag="OO357_001551"
     CDS             complement(30715..30780)
                     /locus_tag="OO357_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886540.1"
                     /translation="METFCYMKWPVRHHKSRRVSH"
     gene            31015..31902
                     /gene="rhtA"
                     /locus_tag="OO357_001552"
     CDS             31015..31902
                     /gene="rhtA"
                     /locus_tag="OO357_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/homoserine exporter RhtA"
                     /protein_id="HCR5886541.1"
                     /translation="MPGSLRKMPVWLPIVILLVAMASIQGGASLAKSLFPLVSAPGVT
                     ALRLALGTLILIAFFKPWRLRFAKEQRLPLLFYGVSLGGMNYLFYLSIQTVPLGIAVA
                     LEFTGPLAVALFSSRRPVDFVWVVLAVLGLWFLLPLGQDVSHVDLTGCALALGAGACW
                     AIYILSGQRAGAEHGPATVAIGSLIAALIFVPIGALQAGEALWHWSVIPLGLAVVILS
                     TALPYSLEMIALTRLPTRTFGTLMSMEPALAAVSGMIFLGETLTPIQLLALGAIIAAS
                     MGSTLTVRKESKIKELDIN"
     gene            32201..32704
                     /gene="dps"
                     /locus_tag="OO357_001553"
                     /gene_synonym="pexB"
     CDS             32201..32704
                     /gene="dps"
                     /locus_tag="OO357_001553"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001730644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HCR5886542.1"
                     /translation="MSTAKLVKSKATNLLYTRNDVSDSEKKATVELLNRQVIQFIDLS
                     LITKQAHWNMRGANFIAVHEMLDGFRTALIDHLDTMAERAVQLGGVALGTTQVINSKT
                     PLKSYPLDIHNVQDHLKELADRYAIVANDVRKAIGEAKDDDTADILTAASRDLDKFLW
                     FIESNIE"
     gene            33108..33854
                     /gene="glnH"
                     /locus_tag="OO357_001554"
     CDS             33108..33854
                     /gene="glnH"
                     /locus_tag="OO357_001554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter substrate-binding
                     protein GlnH"
                     /protein_id="HCR5886543.1"
                     /translation="MKSVLKVSLAALTLAFAVSSHAADKKLVVATDTAFVPFEFKQGD
                     KYVGFDVDLWAAIAKELKLDYELKPMDFSGIIPALQTKNVDLALAGITITDERKKAID
                     FSDGYYKSGLLVMVKANNNDVKSVKDLDGKVVAVKSGTGSVDYAKANIKTKDLRQFPN
                     IDNAYMELGTNRADAVLHDTPNILYFIKTAGNGQFKAVGDSLEAQQYGIAFPKGSDEL
                     RDKVNGALKTLRENGTYNEIYKKWFSTEPK"
     gene            33993..34652
                     /gene="glnP"
                     /locus_tag="OO357_001555"
     CDS             33993..34652
                     /gene="glnP"
                     /locus_tag="OO357_001555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015367581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter permease GlnP"
                     /protein_id="HCR5886544.1"
                     /translation="MQFDWSAIWPAIPLLIEGAKMTLWISVLGLAGGLVIGLLAGFAR
                     TFGGWIANHVALVFIEVIRGTPIVVQVMFIYFALPMAFNDLRIDPFTAAVVTIMINSG
                     AYIAEITRGAVLSIHKGFREAGLALGLSRWETIRYVILPLALRRMLPPLGNQWIISIK
                     DTSLFIVIGVAELTRQGQEIIAGNFRALEIWSAVAVFYLIITLVLSFILRRLERRMKI
                     L"
     gene            34649..35371
                     /gene="glnQ"
                     /locus_tag="OO357_001556"
     CDS             34649..35371
                     /gene="glnQ"
                     /locus_tag="OO357_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000569103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine ABC transporter ATP-binding protein
                     GlnQ"
                     /protein_id="HCR5886545.1"
                     /translation="MIEFKNVSKHFGPTQVLHNIDLNIAQGEVVVIIGPSGSGKSTLL
                     RCINKLEEITSGDLIVDGLKVNDPKVDERLIRQEAGMVFQQFYLFPHLTALGNVMFGP
                     LRVRGANKEEAEKLARELLAKVGLAERAHHYPSELSGGQQQRVAIARALAVKPKMMLF
                     DEPTSALDPELRHEVLKVMQDLAEEGMTMVIVTHEIGFAEKVASRLIFIDKGRIAEDG
                     NPQVLIKNPPSQRLQEFLQHVS"
     gene            35632..37856
                     /gene="ybiO"
                     /locus_tag="OO357_001557"
                     /pseudo
     CDS             35632..37856
                     /gene="ybiO"
                     /locus_tag="OO357_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415329.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive channel protein"
     gene            complement(37853..38779)
                     /gene="rlmF"
                     /locus_tag="OO357_001558"
     CDS             complement(37853..38779)
                     /gene="rlmF"
                     /locus_tag="OO357_001558"
                     /EC_number="2.1.1.181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415328.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(1618)-N(6))-methyltransferase
                     RlmF"
                     /protein_id="HCR5886546.1"
                     /translation="MSAQKPGLHPRNRHHSRYDLATLCQVNPELRQFLTLTPAGEQSV
                     DFANPLAVKALNKALLAHFYAVANWDIPDGFLCPPVPGRADYIHHLADLLAEASGTIP
                     ANASILDIGVGANCIYPLIGVHEYGWRFTGSETSSQALSSAQAIISANPGLNRAIRLR
                     RQKESGAIFNGIIHKNEQYDATLCNPPFHDSAAAARAGSERKRRNLGLNKDDALNFGG
                     QQQELWCEGGEVNFIKKMIEESKGFAKQVMWFTSLVSRGENLPPLYRALTDVGAVKVV
                     KKEMAQGQKQSRFIAWTFMNDEQRRRFVNRQR"
     gene            39055..39315
                     /locus_tag="OO357_001559"
     CDS             39055..39315
                     /locus_tag="OO357_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5886547.1"
                     /translation="MKKCLTLLIATVLSGISLTAYAAQPMSNLDSGQLRPAGTVSATG
                     ASNLSDLEDKLAEKAREQGAKGYVINSAGGNDQMLGTATIYK"
     gene            39580..>39882
                     /locus_tag="OO357_001560"
     CDS             39580..>39882
                     /locus_tag="OO357_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5886548.1"
                     /translation="MENNRNFPARQFHSLTFFAGLCIGITPVAQALAAEGQANADDTL
                     VVEASTPSLYAPQQSADPKFSRPVADTTRTMTVISEQVIKDQGATNLTDALKNVPGV"
BASE COUNT         9937 a        10524 c         9716 g         9705 t
ORIGIN      
        1 aggcatcttt accgaccgtc accatatcgg tgagtttaat cttatcggcc ttaagcgcct
       61 gcccaaccac atagctggtc atgattttag tcaggctcgc aggatccagt ttctcatccg
      121 cattgccttc tgccagcact ttaccgctgg cgtaatccat taaaatccat gcacgcgcat
      181 ccacgctcgg cgcttcaacg gtttgttcgg ccgcgaatgc cgttggggca aaaaggaata
      241 aaaatgcaga acccgctgca agaccacgaa ggagagagga gtattgcgtc ataaatgcca
      301 cccgagtatc cattcaaaaa aatacgttat atcgccgtat gataagttcg gtgattaata
      361 acgcacacac aaacttaaag aaaccgtaag ttggtaaagt ttttaaagtt tatgcaatat
      421 cctgccgctt cgctgcttct ggtgtcattt ttttgccagc tgttgcttaa aagttaactt
      481 tacttcacca tcggagtcct gttcagtctg tggctgcctg tcggaaaagg ggtgagtatg
      541 attacgctgt ggggtcggaa taattcaact aacgtaaaaa aagtattgct gacgctcgaa
      601 gaactggaac taccttatga gcaaattctc gcgggccgtg agtttgggat aaatcacgat
      661 gctgattttc tggcgatgaa ccctaacggg ctggtgccgt tgttgcgtga cgacgaaagt
      721 gatctcattc tttgggaatc aaacgccatt gtccgctatc tggcggcaca gtacgggcaa
      781 aaacgcctgt ggatcgactc accggcacgt cgtgcggaag cggaaaaatg gatggactgg
      841 gcaaaccaga cgctcagtaa tgctcatcgc gggatcctga tgggattagt cagaacacca
      901 ccggaagagc gcgatcaggc cgccattgat gccagttgca aagagtgcga cgccctgttt
      961 gccctgctcg atgcggaact ggcaaaagta aaatggttct ctggcgacga gtttggtgtg
     1021 ggcgatatcg ctatcgcacc gttcatctac aatttgttta acgttggcct gacctggaca
     1081 ccgcgtccaa atctgcaacg ctggtatcag caactcactg aacgccccgc agtgcgcaaa
     1141 gtggtgatga ttcccgttag ctaattgcgt gggctaactt taagtaattc cccactggac
     1201 tcgtcggtga gaacgtataa ataaccgtcg ggtccagtgc gaacatcacg aattcgctgc
     1261 cctctgtccg ttaaaatacg gccatcttct gtcactttgt cgccgttgac gctcatcaca
     1321 atgacatctt tatctttcag ctcccaataa ataatttttg ctgccactgg gggaatttat
     1381 cgctgttata gaaggccatg ccgctcacag cgggcgaatc tttccagtaa aaaacaggtt
     1441 gctcggtccc ggcgacgatc tcccctttcg cttccggtat cttaaagcct gaatagttga
     1501 ttccccaggt tgccagcggc cagccgtagt ttttgccttt ttgcgggata ttaatttcat
     1561 caccaccgcg cgggccatgt tcattcagcc acagtgcatt actccacgga ttcatcgcca
     1621 ttccttgcgg attacgaatg ccataagacc agatctcggc gcgcgcaccg gattccttta
     1681 taaaaggatt atcatccggg atttcgccct ggtcggtcag acgcaccagt ttgccctgta
     1741 atttatccag atcctgcgcc gtcgggcgct gattgttttc gcccagagca ataaaaagat
     1801 aacctttacc gtcgaatacc agccgcccgc caaaatggtt gccggtagac agttttggca
     1861 tctggcggaa gacggtgcgg aagtcggtca cttttgagag atcatcactt aagcggccat
     1921 aacccacagc agttccggct ttgccatcat cgccaacttc ggaataactt aaccagatgc
     1981 ggcgagactg agcaaaatca ggcgctaaaa ccacgtccag caggccgccc tgcccgtgcg
     2041 cccaaacgtc cggaactccg gaaagcggcg cagataatcc ttttcctgct tgccagtgac
     2101 gcaactcgcc gccgcgcaga gtgattaaca taccgtgatt atcgggtaaa aaggccagtg
     2161 cccagggatg gtcgagtttg tcttgcagta cttcgacatt taccgttgca ggagccgccc
     2221 agagagcgga agaaagacaa ataaggggca caaggaaaaa ggattgtcga tgcataacgc
     2281 ctccggattg actctggctt aaagcgtagt cagtggagga gataacaaat tcatttttac
     2341 aaaaacttaa acatgaaggg ggagacgctt tctccccctt agttttcagg ccttctcaag
     2401 catggcgtgc ttctgcaggc tctggatact cagcgttaag ctcatcagac aattttcaag
     2461 cttatcggcg ttgacggtaa taacagtcgg gcaatcatgg tgcccactca tcaaacatac
     2521 tgcggctgtc gctaatgctt cttcagcatg atgaagagca ctccactctt cctgatccag
     2581 atgaagattc aaccgcagcg atttatcgtg cagttcgcga ttcagtttaa aaaagttatc
     2641 tcgtagatga ttgctttcgc tgacggacat gtatcctttt gccttcctca gttgcacgta
     2701 tgcagcgagg tcaacgcgtg cgtcgatcaa ccggttcagc agatcgattc gctgtctgtc
     2761 aacgaacata cttcgatcct cctctttcca gccttgttgc aattaaatag tatggctatt
     2821 ttttgagcaa aatgctgtcc aggatcaatt cttcacgccg aattaacaaa atttacattt
     2881 ccttcgcctc cgccagcgaa tgagattgcg atacaaattt tatccagaac tctgtagaat
     2941 ccctgccctg acctttcctg taactgaata ttttttaagc tatgagcaaa gtaactcccc
     3001 agccgaaaat cggctttgtt tcccttggct gtccgaaaaa ccttgtcgat tcagagcgta
     3061 ttctcaccga actccgcact gaaggttatg acgtggtacc gagctatgac gatgcgaaca
     3121 tggtgatcgt caacacctgc ggctttattg acagcgcggt acaagaatca ctggaagcca
     3181 ttggtgaagc gttgaatgaa aacggcaagg taattgtgac cggttgtctg ggggcaaaag
     3241 aagatcagat ccgcgaagtc cacccgaaag tgctggaaat caccgggcct catagctatg
     3301 agcaggttct ggagcacgtt catcactacg tgccaaaacc gaaacacaac ccattcctga
     3361 gcctggtgcc agaacaaggt gtgaaactga cgccgcgtca ttatgcctat ctgaaaattt
     3421 cggaaggctg taatcaccgc tgcaccttct gcattattcc gtctatgcgc ggcgacctga
     3481 tgagccgtcc gattggcgaa gtgttaagtg aagcgaaacg tctggtagat gcgggcgtta
     3541 aagagattct ggtgatctcg caggatactt ccgcctatgg cgttgatgtt aaacatcgta
     3601 ctggcttcca caacggcgag ccggtaaaaa ccagcatggt cagcctgtgc gaacagttat
     3661 cgaaactggg gatctggaca cgtctgcact acgtttaccc ttatccgcat gtggacgacg
     3721 tcatcccact gatggcagaa ggcaaaatcc tgccgtatct ggacattccg ttgcagcacg
     3781 ccagcccgcg cattctcaaa ctgatgaagc gtccgggttc tgtagatcgc caactggcgc
     3841 gcatcaaaca gtggcgcgaa atctgcccgg aactgaccct acgctcaacc tttattgtcg
     3901 gcttccctgg cgagacggaa gaagatttcc agatgctact cgacttcctg aaagaagcac
     3961 gtctggatcg cgttggctgc tttaaataca gcccggttga aggtgcagac gccaatgccc
     4021 tgcctgacca ggttccggaa gaagtgaaag aagaacgctg gaaccgtttc atgcagctgc
     4081 agcagcagat ttccgccgag cgcctgcaag agaaagtggg ccgtgaaatt ctggtgatta
     4141 tcgacgaagt ggacgaagaa ggcgcgattg gtcgcagcat ggcagatgca ccggaaatcg
     4201 acggcgcggt ttatctcaat ggtgaaacca acgttaagcc gggtgatatc ctgcgtgtga
     4261 aagtcgagca cgccgatgag tacgatttgt ggggtagccg ggtttaatta attcaggccg
     4321 ggcaaaatca tttgtccggc ctgcgaataa tgattactgc cagctaatct gtgccctgcc
     4381 gtttttctta ctcttataca gccgctcatc cgccagcatt ttcaagcgtt cgacggtagt
     4441 acattcattt gaatgcgcta caccgatact ggtgcttaaa ttaatgcttc ccacctggaa
     4501 atgaacgatt tcttttagta tcgatagcaa ctcttccagc gtgccgccag tataaatcac
     4561 cgcaaattcg tcacctccga agcggtagac tttaccttta tctaccggga tctgtgattt
     4621 aaggattttc accactttca tcaacacttc atcgcccagc aaatgaccga aggtgtcgtt
     4681 aatgtttttg aatttatcga cgtcaaacag acacaaataa ccgccttttt ccggcagcgc
     4741 ttttaaatct tcatcaaagg attttctgcc cattgctttg gtcaggtcgt ccaggaacgc
     4801 cgagttatac atactgtaga gttttttgac atcggcagcc gaaaagaaaa gcccgccatc
     4861 cagtacgcgt tcaaaatggt tattaatgat ggcttctttc tccacaatac cacgattaat
     4921 cactctgcgt accagataga ggcaactggc acccaatatc actgccacca atgcggcaaa
     4981 gaaaataatc aatgtacaaa cccgcaaata aacatctttt ttatacgcaa atacgtacaa
     5041 tccggtcggc aaataggcga agtgctccat cgcttccgaa acggtgaaga tggcgttttt
     5101 agcattatat tcatcaccgg tcaccggttt accgttctta aatagagaga acatcaggtt
     5161 ctcgctgtcc atcaccagcg attttgacag taaagaaaaa cgctccagac caagcaaaaa
     5221 tatcagacta cgattgtgag ttaccggata gaggtaatag acgaaggttt taccgtcaaa
     5281 agagataaac cctgtatttt tgacatgtcg atacttcaca acctgcataa tatctgcaca
     5341 ataatttttc tcgaactcct ttacctgaat acattcgtca ctggaaatca gataaggacc
     5401 gacatcgtac aaataagagg gggaaaccga gtcggcgtaa gctgtgcgca aaccaaccaa
     5461 cagctgttcc tccgtgggat ttagcgtgtt gataagatca attgccacgc tacgccgata
     5521 ttcagcaata tttgccaccg caaaagcagt atcaataacg gactgtttca ggctatacac
     5581 ccgttccttt accatttgag tgtagatccc gcaggcaact gctgaaatca tgataaaaat
     5641 cagcagactg actgtaagta cgaacttatt gattctggac atacgtaaac tactctttta
     5701 ctaattttcc acttttatcc caagcggaga atggcatggg tttctggtac agatacccct
     5761 gagcctgaac acatcctaat ttaattaatt cgcgcgcaac gtcttcgctc tctacgcctt
     5821 ccgcaatcac cgggatccgg ttatagcgtg ccagatcgaa aataatgcgc acaatttttc
     5881 ccgcagtacc gaaaatatcg ccggtaaatt gcgagtccac ctttatgctg tctggattaa
     5941 tttcataaaa acgtttcagg tttgacaaac cggtgccaaa gtcatcaatc gatatccgta
     6001 ccccttgttt acgcagttgt tcaatattga gataaaaatg ttcattaatt cgctcgatca
     6061 cggtttcagt gatctcaaca cagaggctgt tcgccttcag gccattttct ttcatgatgg
     6121 tggtaaaacg ctcatgaaag tgcggttcgt ttaactgcca gggcgaaaca ttaatatgca
     6181 gtaagaaatc atccttcagc gacgcacgtt gactaaaatg tctgaattaa cccattgccg
     6241 ttttaataat ctgataaccc agctcattga taaacccgat atcttcagca atggtaatga
     6301 acttcagcgg tgaaatgatc ccccgctccg cagaaaccca acggcacaac gcctccgcgc
     6361 tcagaatggc gcgggtgtta atatcaacga tcggttgata gacgaggaat agttcgccaa
     6421 tttccattgc atggcgaata ttacgcgcaa tccagatatt ctcttctatt tcgttgagtt
     6481 tcgcgtcatc acagaaaata aactcggaga acatatggtc acggatactg gaaatcgcca
     6541 gtttcagttt ttctgcgtaa ctccaggcgt tttccccctg caaaggttca caaatggcag
     6601 acttatggat gcgcagattg cggtagccaa agctgctgtc aaaatcgttt accatcgcta
     6661 ttttacgggt aaatgtctgt acattttccc gacacagaag taaatagaga tcggcattga
     6721 atgtcacgat atcgcagcat tgcgcatatt tctcggcaat tcgctgacac aatttcctga
     6781 cgattttatt gcgttcatca acaccaaaca ccgcctccac ggcttccaga ccacacagtt
     6841 tgatgatccc cacagccaga tactgattat tgttggactc aataatcgcc atatttttaa
     6901 taaaaccgct ttcctgatcg taagagatca gtgagagcaa tttatttgac cgttcaattt
     6961 cggcatcttt catttccgcg atcgaatttt tgatgctgat aatctctttc gcatcgcctt
     7021 cgcgaatttt cagagcctcg atctgatccg ttgagtcggg caaaaacgcc agcgtattaa
     7081 ccagctcggt catttcagaa aattgccgac ggaacacacg agtggtcatg aaatatacca
     7141 ccagcaacac gataataatg aacgcaaacg gtagataaaa aagtttatct gatgcgttca
     7201 ataaattacc gccaggataa taaaaataga tttcgaacaa tgcattattt ggcactgctg
     7261 atgtctttgc aatatactcc gtatcgacag aatcaataat attataatta ccttcgctat
     7321 tataaataat cagtttttca cgagcgagtt gattcttggc aatgatctca tgaccttgtt
     7381 tcaaaaatag aacttcatca ttagcgctga tttgatcaag atactcgttc agataccgcc
     7441 agtttaaatc gacatgaatt ttatctaccg ctaacaaatc gtgatctttg ccgttgatac
     7501 gtagcgtaaa agagatacat tttttacccg gacgatcaaa gcaattgtat gccggattcc
     7561 agctgttgtt agagtcatct gaagccaccg accacggtcg tttcgatggg ttaaaatcgg
     7621 caaaaataac ccgccctgac tcgtcgacaa gcggtccatt tacttgttcc tgatgataaa
     7681 caacgatttt atcattcgcc ctccgcgaaa agagataata tttattatca tcaagtacca
     7741 gtcccaccga gtctaaaaat ggcatcattt ccattttcag aatcacggcg cgttgaatct
     7801 cctctgtatt ttttaataaa tacagtgagt cctttaatgt cagggccgaa cgttcaatat
     7861 ctttaaagaa atagccaacg tttttctgaa taagttcaaa gcggctatcg gcacgtttta
     7921 ccgcgcgttc cgataccagt tgatagttaa tcatgaaaaa aagaccaata aatgacagtg
     7981 ctgccaataa aaataaaatt atcagcctta tcttttcgta taaactcagc atgttaatac
     8041 atttaacaaa ataactatct gaacgctgtg ccgctgcaaa aatagtccct tgctgcggct
     8101 ttttaaccaa attacccgat tctacaaaat cagcagaaag ttacaacacc tgcttagggc
     8161 aataatttcc tgtgaggtga ttaccctttc aagcaatatt caaacgtaac tatcctttaa
     8221 ttttcggatc cagcgcatcg cgtaaaccat cgcccaacaa attgaacgcc agtacggtca
     8281 gaaaaatagc cagggccgga aaaacagcga catgcggcgc gataaccata tccgctcgtg
     8341 cctcattgag cattgctccc cactctggtg tcggcggctg cgcaccgagg ccgagaaatg
     8401 acaggctggc ggcagagata atcgaggtgc caatgcgcat ggtgaaaaac accacgatag
     8461 aagagacggt cccaggcagg atatgacgca ataaaatggt catatcgctg gcaccaatac
     8521 tgcgtgctga ctcaataaag gtttgctgtt tcaacaccag cgtgttgccg cgcaccaggc
     8581 gggcaaacgc ggggatggaa aaaatggcga ctgcgataat cacgttagca atgccgcttc
     8641 ccaacaccgc aacaacagcg atcgccagta aaatacccgg gaaggcaaac agcacatcgc
     8701 aaatgcgcat gatcagccga tcccaccagc cttcataata tccagccaac aagcccagca
     8761 acgtccctat cgccacaccg ataaacacgg caaacacgcc cgccgccagc gagatttgcg
     8821 caccaaccag gacacggctg aaaatgtcgc gccccagtga atcgacgcca aaccagtgct
     8881 gcaaagaagg tccgttattc agattgtcat aatcaaaata attttcggcg tcatagggag
     8941 cgatccagcg tgcaaaaatg gccaccacaa tcaataaaat aacgaataat gcggcggtca
     9001 tcgccatatg ctgacggcga aatcgtcgcc agaattcatg ccacggtgta cgtacctggt
     9061 caggtttgac cagtggcatg gcgtttaaca ccgcctgacg tcgccagtta aatagtcgca
     9121 tccttacttg tacctgatag ccgggttaat ggcggcgtaa agcacatcca ccactaagtt
     9181 gataagaata aattccagcg agaaaagcag aatttccgcc tgaatcaccg ggtaatcgcg
     9241 catttctaca gagtcaacga gtaagcgtcc aagtccgggc cagttgaaaa ctttctccac
     9301 cacgatagaa ccaccgagca aaaagccaaa ctgtaagccc atcatggtca ctaccgggat
     9361 catcgcgttg cgtagcccgt gtttgaggac aacccaggtt tcgctcaccc ctttcgccct
     9421 cgcggtacgc atataatctt cgcttaaaac gtcgacaaat gacgcacggg taaagcgcgc
     9481 catcacggcg gccactgccg cgccgagcgt cagggagggt aaaatgtagt gctgccaact
     9541 gtctgctccc acagtaggca gccagcccag ttcaacggag aatacctgaa ttaaaaacat
     9601 ccccagcgca aatgccggaa acgagatccc cgacaccgca atggtcatac tcaatcgatc
     9661 cggccaacga ttacgccaga cggcggcgat aattcccgcc gccataccaa atatcaccgc
     9721 ccagaccata ctggttatgg tcagccatag cgttggcata aagcggctgg caatctcatc
     9781 ggcaaccgga cgacgcgaca ccatcgacag gccaaaatcc ccctgcacag cattgctgat
     9841 atagtgccag aactggtgat acagcggctg atccaacccc agctgctgac gcaccagttc
     9901 tataacctgc gcatcagctt cgggcccggc aatcaatcgc gccggatcgc cgggcagcat
     9961 gtggacaaat aaaaacacca gcaccgagac gataaacagc gtcggaatca accccagtaa
    10021 tcgtttgata acgtaataaa gcattccact ccctgcgttg cttattgcaa atccgcgtct
    10081 tcaaagctga agccggtatc tggcatgatc caaaaaccgg tcaggttttt actgtgtgcc
    10141 gacaccagtt tttctaccac cagcgggatc cacggcgatt cttgccagat gatatcctgc
    10201 gccgccttat ataagcgggt cttttccgcc ggatcattcg ttttcagtgc ctgagccagg
    10261 aagtcatcca cctgtttatt gctgtaaaac gcggtattaa acagcgtcgg tggccagttt
    10321 tgcgaggcaa acagcggcga tagtgcccag tcggcttcgc cggttgaagc cgaccagcca
    10381 gtgtagaaca tccgcacgcc actctctttt tgccctttac cttcaacttc tgccgcccgc
    10441 tgtccggcat ccatcgcggt cacctgggct ttaatcccga cctgcgctaa ctgctgctgg
    10501 gtaaattgca gcactttctg cgcggtgctg tggttatgtg acgaccacag cgtggtactg
    10561 aaaccgttgg gatatcccgc ctctttcagt aattcgcgcg ctttcactgg atcgtaaggc
    10621 cacggtttat aactttgcgc gtaggcgata cttggcggta ccacaccagt agctggagtt
    10681 gcatagcccg caaaggcaac tttcaccagc gccggacggt taatggcgta attcagcgcc
    10741 tcacggacct tcgggttatc gaacggcttt tgcgtcacgt tcatactgat ataacgctgc
    10801 ataattgacg gactggccat caactcgata tttttgtttt tctccagcag tgtggcctgc
    10861 tcgtagggaa tggggaaagc aaactgcgct tcaccggttt gcagcattgc cgcgcgggtg
    10921 ttgttatccg ccaccggacg ccaggttatg ctgtccagtt tgggcaatcc tggctgccag
    10981 taacccgcga attttttcac cttcacaaaa tcggtctgat tccaggtatc cagttcatac
    11041 ggtccggttc ccaccggatg aaaaccaatc tccttgccat atttttccag cgctgtcggt
    11101 gaaatcatcg cggtcgccgg atgggcaaga atattaataa acgctgagaa cggctgtttg
    11161 agggtaatct ttaccgttgt cggatcgatc gcctccgttt tggcaatatt cttatacagg
    11221 ttatagcgtt taagatgatt cgccggatcg ctggcccggt ccagattcgc ttttaccgcc
    11281 actgcgttga aatcggtgcc atcctggaat ttaattcctt cccgcaattt cacggtgtaa
    11341 gtaatgccgt catcggaaac ggtataactc tccgccagca cgtttttcag tttcatctct
    11401 ttatccagac cgaacagccc ctggtaaaac gatttcgcta cggcctgaga taacgtgtca
    11461 ttcgcgtcat acggatcgag cgtggtgaaa ttcgatccta ccgccaccac cacatctttg
    11521 gcagcgaatg cacaagatgc catcaacgct gtcgcaatgc ccagcgccac taacccacta
    11581 cggtgtacag ctcttgccat tttattctcc gcctgaatgt tatctacgca tgaatgcgta
    11641 ttctgattgt ggttgtgcga cgtaatgccc cggcccgacg cattgcaacg agacggctgc
    11701 cacctcttcg ccacgcagat gaatattgct gggaagatcg tccgacagca gtacacgctg
    11761 cggtcgttgt cgggacggtt cagcgaccgg aactgccgcc agtaatttac gcgtataagg
    11821 atgctgcggg ttttcgaaga ccgcgcaccg tgaaccaatt tcaacaattt gcccgagata
    11881 catcaccgcc acacgatgac taatccgctc taccacagcc atatcgtggg agataaacag
    11941 atacgcaatg ccgaaatcac gctggagatc gagcaacaag ttgataatct gcccgcgaat
    12001 agaaacatcc agcgccgaaa cggcttcgtc ggcaatgatc acttttggat tcaatgccaa
    12061 cgcgcgagca atgcagatgc gctggcgctg accgccggaa aactcatgcg ggtaacgcca
    12121 ggcatgttca ggtaacaggc ccacgcgctc cagcaaccac gcaacgcgtg cagccgcgtc
    12181 tttacctggc aataaaccgt gtacacgcag cggttcgata atcgaatcac cgatggtctg
    12241 acgtgggtcc agcgaagcgt aagggtcctg aaaaataaac tgaatatccc ggcgcaatgc
    12301 ctgaagtttg ccaggtgaca aggtatcgat tcgctgaccg ttaaagataa tttcgccgcc
    12361 ctgcgattcg accaggcgca gcaacgcccg cccggtagtg gatttaccgc tgccagactc
    12421 gcccaccagc gatagcgttt cgccaggcca gagatcaaaa ctgactttct caacggcatg
    12481 cacttcccgc gttacgcgat tcaacaaacc gctgcgcaaa gggaaacggg tgacaagatt
    12541 acgcacccgt aaaacaggtt cgccatccac caccgttttc tgctcgatgg ggggttcctg
    12601 tttcgctgga tgttcaagcg atatcaatgg gaaacgtcgg ggataatcta accctttcat
    12661 cgcaccaagt tgcggaacag cagctaacag cgcacgggtg taaggatgtt gcggtgcatg
    12721 aaaaatctgt tcgacggtac ccgtttccac cgcctcgccc tgatacatca ccagtacccg
    12781 atcggcaatc tctgccacca cgcccatatc gtgagtgata aagataacgc ccatcgacat
    12841 ctctttttgc aatactttga ttaattgcag gatctgcgcc tgaatagtga catccagcgc
    12901 ggtggttggc tcatcggcaa tcagcaccgc cgggcggcat gacagcgcca tcgcaatcat
    12961 cactcgctgg cgcatcccgc cagagagttg atgcgaataa cgtgaaagaa tggtttgtgc
    13021 ctccggaatg cgtacctgat ccagcatccg cttcgcctcg accatcgctt cttcacgact
    13081 ggcgttctga tgcagacgaa ttgattcggc aatctgttca cccacagtaa atactgggtt
    13141 cagcgatgtc atcggctcct gaaatatcat cgccatatcc gcaccgcgca catggcgcat
    13201 ttgtgcagcg ctctgctcgc taagttcaat cacatcacga ctgcgccgcc gcaacagcat
    13261 tttatcgcac tgtactaaac cgcccgcctg ttccaacaga cgcatcaatg ccagcgcagt
    13321 cactgactta ccggagccgg attcgcctac aattgccagc gtctcaccgc gttgcagact
    13381 aaaagagaga ttgcggaccg cagctatttt ctgctggtcc tgcataaagg caatattaag
    13441 attttcaacc gccagcacat taccggcatc aagttcatca ctgtgtggca acggtgtccc
    13501 ctttttcacg gtagataccg gtggttggcg tatcgcctgc gtagccccag gcgcgataca
    13561 ttccttcggt gttaaacggt agcgcgacat tcccttcatg gtcgatagcg attaagccac
    13621 cgctaccgcc aagcgcaggg agtttttcca ttactacccg ctcgcaggct tccgcgagac
    13681 ttaatccgcc gtaatccatt aacgcggcga tgtcatatgc cgccagcgcg cggatgaaga
    13741 cttcgcctgt gccggtacaa gaaaccgcca cactggcgtt attggcgtag catccggcac
    13801 ccactaaggg actatcgcca actcgtccgg gtaatttatt ggtcattccg cctgtggacg
    13861 tggctgccgc cagattgccg tctaaatcca acgccacggc ccccacggtg cccattttct
    13921 gtttttcatc cagtggcgca ccgctatggt cgaggactgt tgccccttcc tcgcgcgctg
    13981 ccagtagttg ttcataacgc aaaggcgtgg agaaaatctc cggtgagacg cgctccatgc
    14041 catgagcaaa cgcaaaattt tctgcccctt cgccaatcat catcacatgc gggctttgct
    14101 ccatcaccag ccgggcggca agaaccggat tacgcagatg actaacgccc gccaccgcac
    14161 cggctttcag ggtgttacca tccatcacac aggcgtccag ttcatgggtt tcatcacgcg
    14221 taaagacagc gccaattccg gcgttaaaca gtggacactc ttccagcaga cgcaccgctt
    14281 ccgtcaccac atccagcgca ctttcgccgg cttccagcat tttctgcccg gtttcaacaa
    14341 tggcagacaa cgcctcgatg tagcgtaatt cctgttgcag actcatctgc gcgcggctaa
    14401 ttgcacctgc gccaccatga attgcaatga ctgctttgcc catagtattc gtccattaag
    14461 ggtggctcgc cacttttgct ataaatatca gaatttttat gaattactca ttcaattttt
    14521 gaatatagaa agatggatat ggcatgtaaa ggcaggagcc aacgctacgc ccgaggtggc
    14581 atacagtcta tggcgttttt ctgacataat aggcaaattc gattttgcct ccgcaggagt
    14641 gttttcatgg aatttaccac cggattgatg tcgctcgaca ccgcgcttaa tgagatgctt
    14701 tctcgcgtta ccccactgac cacccaggaa acgctgccac tggttcagtg ttttggtcgt
    14761 attctggcga gcgatgtcgt ttcgccactg gatgtcccgg ggtttgataa ctccgctatg
    14821 gacggctacg cggtgcgttt agccgatatt gcctccgggc aaccgctgcc cgttgccggt
    14881 aaatcctttg ccggtcagcc ataccatggt gaatggcctg cgggtacctg cattcgtatt
    14941 atgaccggtg cgccggtgcc ggaaggctgc gaagcggtgg tgatgcagga gcagactgaa
    15001 caaacggaca atggcgtgcg ttttactgct gaagtgcgta gcgggcaaaa tattcgccgt
    15061 cgcggtgaag atatctctgc aggtgcggtt gttttcccgg cgggaactcg cctgactacc
    15121 gcagagctgc cagtaattgc ttcactgggg attgccgaag ttccggtgat tcgtaaagtg
    15181 cgtgtagcgc ttttttctac cggtgatgaa ctccagttgc ccggtcagcc gctgggcgac
    15241 ggccaaatct acgacaccaa ccgtctcgcc gtacacctga tgttagaaca gctgggttgc
    15301 gaggtaatca acttagggat tatccgcgac gatccacatg ccctgcgcgc cgcatttatt
    15361 gaagccgaca gccaggcgga tgtagtgatc agttccggcg gtgtttcagt gggtgaagcg
    15421 gattacacca aaatgattct tgaagagctg ggggagatcg ccttctggaa gctggcgatt
    15481 aaaccaggta aaccgttcgc gttcggtaaa ctcagcaata gctggttctg cggcctgccg
    15541 ggcaacccgg tttcagcgac gctgaccttc tatcaactgg tacagccttt gctggcaaaa
    15601 ctaagcggca acaccgccag cggcctgccc gcgcgccagc gcgtacgcac agcgtcccgc
    15661 ctgaaaaaaa cgccaggacg tcttgatttc cagcgcggcg tgctgcaacg caacgccgac
    15721 ggtgaacttg aagtgacgac caccggacat cagggttcac atatatttag ctcctttagt
    15781 ctcggcaact gctttatcgt gctggaacgc gatcgcggca atgtggacgt gcgcgaatgg
    15841 gtggaagtag aaccgtttaa cgcgttgttc ggaggcctgt aatggcggaa ctcagcgatc
    15901 aggagatgct gcgctacaac cggcaaatca ttctgcgcgg ttttgatttt gacggtcagg
    15961 aggcgctaaa agattctcgt gtgctggtag tcggcctcgg tggactcggc tgcgcagctt
    16021 cgcagtatct ggcaagcgcc ggtgtcggca acctgacgct gctcgacttc gacacggttt
    16081 cgctctcgaa tctgcaacgc cagacactgc acagtgatgc cacggtcggg caaccgaagg
    16141 tggaatccgc ccgtgacgcc ctgacgcgga tcaacccaca tatcgcgatt acgccagtca
    16201 atgcactgct agatgacgca gaacttgcag cattgattgc tgaacacgat ctggtgctcg
    16261 actgtacgga taacgttgcg gtacgtaatc aactgaacgc aggctgtttt gccgcgaagg
    16321 tgccgctggt ttccggcgcg gcaattcgta tggaagggca aatcacggtc ttcacttatc
    16381 aggacggcga accgtgctat cgctgcctta gccgtttgtt tggcgagaac gcgttaacct
    16441 gcgtagaagc aggcgtgatg gcaccgttga taggcgtaat tggttcgttg caggcaatgg
    16501 aagcgatcaa actgctggca ggttatggca aaccggccag cgggaaaatc gtcatgtacg
    16561 atgcgatgac ctgtcagttt cgcgaaatga aactgatgcg caatccgggg tgtgaggtgt
    16621 gcgggcagta attgccacct ccaagcctga taagacgcga caagcgtcgc atcaggcatt
    16681 tgctccagac gccagatgcg gcgtgaacgc cttatctggc ctacggttcg ggcacagatt
    16741 tccaagcgcg gcaggcatcg gtgcactccg ccacgtcatc agcaatttat gaggatgcag
    16801 aacgtccggt taaatcgacg ttctgccaaa cgctccctgc cagtcctgct caaacttcgt
    16861 cacagcggct tccaccgccg gatagctaat catctgttgt gccacatcca gtggcagagt
    16921 aattgattca catccggcca gtaagcagtc cagcgcctga cgcggggttt tgaaactcgc
    16981 tgccagcact ttcgcctgcg gcgcatgcat tttcaataac tggtgtaagt cggtcacagt
    17041 ctgaatgccg ctaccgccct gagcatcaat acgattaacg taaggcgcaa catattccgc
    17101 acctgccagc gccgacagca gcccttgtgc cgcgccatat accgcggttc ccagcgtcgg
    17161 aatcccttcc gcttttaaca tcttaatcgc tgccagcccc tcggctgtca ccggaacttt
    17221 caccacgata tccgcaataa tagaacgcag cttaagcgcg tcattcacca tcccttcagc
    17281 agtggtagcc attacctggg caaacagacg cccctgaccg cccatcgctt catgaagttg
    17341 cggaagcaca acatccagcg gttttttacc cgcggcgata atgcttgggt tagtggtcac
    17401 acccgccagc ggaaaaatac gtgacagcgc cttcaccgca acaacgtctg aagtatccag
    17461 atacagttcc ataaccatcc tcaaaatgta ctttttgtct gaatgtagac tatcacggga
    17521 aaactctctg aacagttgac gcacatcaat ctaactttca ttcgaaagta ttttaatgtt
    17581 tgaacgaaac agagaggcaa ctatgatttt caatattcag cgctactcga cccatgatgg
    17641 ccccggtatc cgcacggtcg tatttcttaa aggctgttcg ctgggctgcc gctggtgtca
    17701 gaacccggaa agccgcgccc gtacgcagga tctgctgtat gacgcacgac tgtgtctgga
    17761 aggctgcgag ctgtgcgcta aggccgcgcc ggaagtgatt gagcgcgcgc tgaatggttt
    17821 gcttattcat cgggaaaagt taaccccgga gcatctgacg gcgttaaccg actgctgtcc
    17881 gacacaggcg ttaaccgtgt gtggtgaagt gaaaagcgtt gaggagatca tggcgaccgt
    17941 tctgcgcgat aaaccgtttt acgatcgcag cggcggcggc ttaacgcttt cgggtggtga
    18001 acccttcatg cagccggaaa tggcgacggc gctgctgcaa gcctgccacg aggcaggcat
    18061 tcatactgcg gtagaaacct gtctgcatgt gccgtggaaa tatatcgccc cttctctgcc
    18121 ctatatcgat ctgtttcttg ccgatttaaa acacgttgcc gacgcgccgt ttaaacagtg
    18181 gaccgacggt aacgccgcca gggtgctgga taacctgaaa aaactcgccg cagcgggcaa
    18241 aaaaatcatt atccgcgtgc cgctgattca gggctttaat gccgacgaaa cctctgtaaa
    18301 agccattacc gattttgccg ccgacgagct gcacgttggc gaaattcatt ttctgcccta
    18361 ccacacgctg ggcatcaaca aatatcactt acttaatctg ccctatgacg ccccggaaaa
    18421 accgcttgat gcgccagagc tgctcgattt tgcccagcag tatgcctgcc agaaagggtt
    18481 aaccgcgacc ttacgaggat aacaaccatg accacactga aactggacac gctcagcgac
    18541 cgcattaaag cgcacaaaaa tgcgctggtg catattgtga agccgccagt ctgtaccgag
    18601 cgcgcgcagc actataccga gatgtatcaa caacatctcg ataagccgat cccggtacgt
    18661 cgcgcgctgg cactggcgca tcacctggcg aatcgcacca tctggatcaa acacgatgag
    18721 ttgatcattg gcaaccaggc aagcgaagtt cgcgccgcgc cgatcttccc ggaatatact
    18781 gtctcgtgga tcgaaaaaga gattgatgat ctggcagatc gtcccggtgc tggctttgcg
    18841 gtgagcgaag agaacaaacg tgttctgcat gaagtgtgcc cgtggtggcg cggtcagacg
    18901 gtacaggatc gctgctatgg catgtttacc gatgagcaaa aaggtctgct ggcgaccggc
    18961 atcattaaag cggaaggcaa tatgacctcc ggcgatgcgc acctggcggt caatttcccg
    19021 ctgctgctgg aaaaagggct tgatggtctg cgtgagaaag tagcggaacg tcgctcgcgc
    19081 atcaatctga cggtgctgga agatttgcac ggcgaacagt tcctgaaagc gattgatatc
    19141 atgctggtgg cagtcagtga acacattgaa cgtttcgctg ccctggcgcg tgcaatggcc
    19201 gcgaccgaaa cccgcgaaag ccgtcgcgat gaactgctgg cgatggcaga aaactgcgat
    19261 cttatcgccc accagccgcc gcagactttc tggcaggcgc tgcaactgtg ttacttcatc
    19321 cagttgattt tgcagattga atctaacggt cactcggtgt cgtttggtcg tatggatcag
    19381 tatctctacc cgtactatcg ccgcgacgtt gaactgaacc agacgctgga tcgcgaacac
    19441 gccatcgaat tgctgcatag ctgctggttg aaattgctgg aagtgaacaa aatccgctcc
    19501 ggctcccact caaaagcctc tgcgggaagt ccgctgtatc agaacgtcac cattggcgga
    19561 caaaatctgg ttgatggtca accaatggac gcggtgaatc cactctctta cgcgatcctc
    19621 gaatcctgcg gtcgcctgcg ttccactcag cctaacctca gcgtgcgtta ccatgcagga
    19681 atgagtaacg atttcctcga cgcctgcgta catgtgatcc gctgcggctt cgggatgccg
    19741 gcattcaaca acgacgaaat cgtgatcccg gaatttatta aactcggtat tgaaccgcag
    19801 gacgcttatg actacgcagc gattggttgt atagaaaccg ccgtcggtgg caaatggggc
    19861 tatcgctgta ccggcatgag ctttatcaac ttcgcccgcg tgatgctggc ggcgctggaa
    19921 ggcgggcgtg atgccaccag cggcaaagtg ttcctgccac aagaaaaagc gttgtcggca
    19981 ggtaacttca acaacttcga tgaagtgatg gacgcgtggg atacgcaaat ccgttactac
    20041 acgcgtaaat caatcgaaat cgaatatgtc gtcgacacca tgctggaaga gaacgtgcac
    20101 gatattctct gctcggcgct ggtggatgac tgtattgagc gagcgaaaag tatcaagcaa
    20161 ggcggcgcga aatatgactg ggtttctggc ctgcaggtcg gtattgccaa cctcggcaac
    20221 agcctggcgg cagtgaagaa actggtgttt gaacagggcg cgattggtca gcaacagctg
    20281 gctgccgcac tggcggatga cttcgatggc ctgactcatg agcaactgcg tcagcggctg
    20341 attaacggtg cgccgaagta cggcaacgac gatgataccg tcgatacgct gctggctcgc
    20401 gcttatcaga cctatatcga cgaactgaaa cagtaccata acccacgcta cggtcgtggt
    20461 ccggttggcg gcaactatta cgcgggtacg tcgtctatct ccgctaacgt accgtttggc
    20521 gcgcaaacta tggcaacgcc ggacgggcgt aaagcccaca ccccgctggc agaaggcgct
    20581 agtccggcct ctggtactga ccatctcggc cctactgcgg tcattggctc agtgggtaaa
    20641 ctgcctacgg cagcgattct cggcggcgtg ttgctcaacc agaaactgaa tccggcaacg
    20701 ctggagaacg aatctgacaa gcagaaactg atgatcctgc tgcgtacctt ctttgaagtg
    20761 cataaaggct ggcatattca gtacaacatc gtttcccgcg aaacgctgct ggaggcgaaa
    20821 aaacatccgg atcagtaccg cgatctggta gtgcgtgtcg cgggttattc cgccttcttc
    20881 accgcgctct ctccagacgc tcaggacgat atcatcgccc gtactgaaca tatgctgtaa
    20941 tctcttcggc ccggctgcgg cgtcgccggg ctaaatcatt cacatcatca atttcatcct
    21001 tactttcatt cgaaatataa tttgtgctct gcgtcacatt gttattacac tgttacggtt
    21061 tcattttcga gccaggagca cacctatgag cgtaaaagtt atcgtcacag atatggacgg
    21121 tacttttctt aacgacgcca aaacgtacaa ccaaccacgt tttatggcgc aatatcagga
    21181 actgaaaaag cgcggcatta agttcgttgt cgccagcggt aatcagtatt accagcttat
    21241 ttcgttcttt cctgagctaa aggatgagat ctcttttgtc gcggaaaacg gcgcactggt
    21301 ttacgaacat ggcaagcaac tgttccacgg cgaactgacc cgacatgaat cgcggattgt
    21361 tattggcgag ttgctaaaag ataagcaact caattttgtc gcctgcggtc tgcaaagtgc
    21421 atatgtcagc gaaaatgccc ccgaagcatt tgtcgcactg atggcaaaac actaccatcg
    21481 cctgaaacct gtaaaagatt atcaggagat tgacgacgta ctgttcaagt tttcgctcaa
    21541 cctgccggat gaacaaatcc cgttagtgat cgacaaacta cacatagcgc tggatggcat
    21601 tatgaaaccc gtcaccagtg gttttggctt tatcgacctg attattcccg gtctacataa
    21661 agcaaacggt atttcgcggt tactgaaacg ctgggatctg tcaccgcaaa atgtggtagc
    21721 gattggcgac agcggtaacg atgcggagat gctgaaaatg gcgcgttatt cctttgcgat
    21781 gggcaatgct gcggaaaaca ttaaacaaat cgcccgttac gctaccgatg ataataatca
    21841 tgaaggcgcg ctgaatgtga ttcaggctgt gctggataac acatcccctt ttaacagctg
    21901 acctctctac cggagccatg cgctccggtt ttcccttctt tttacatcct gtacatcaca
    21961 tcaaatcatt aaacttgcat taacttattt ataacttgta ttgtcacatg caaattagca
    22021 tagtttcgaa tgaaagttaa tgcaaggtgg ttatggcttc tacttttacc agcgacacat
    22081 tgcctgccga tcacaaagca gctatccgtc agatgaagca cgcgctgcgg gcgcagcttg
    22141 gcgatgtcca gcagatcttt aatcagctaa gcgatgacat tgccacgcga gtggctgaaa
    22201 tcaacgcact caaagcacag ggcgatgccg tctggccggt gctgtcttat gccgatatca
    22261 aagcaggtca tgttactgca gagcagcgcg aacagattaa acgtcgcggt tgtgcggtga
    22321 taaaaggcca tttcccccgc gaacaagcgc taggctggga tcagtcgatg ctggactatc
    22381 tgaaccgcaa ccgctttgac gaggtctaca aaggccccgg cgataatttc ttcgggacgc
    22441 tcagcgcttc acgtcccgag atttacccca tctactggtc gcaggcgcaa atgcaggccc
    22501 gccagagtga agaaatggcg aatgcgcagt cgtttctcaa tcgtctgtgg acatttgaaa
    22561 gtgatggaaa gcaatggttt aacccggatg tgagcgtcat ctaccctgac cgtatccgcc
    22621 gccttccgcc cggaacgacc tccaaaggtc ttggagcgca taccgactcc ggggcgctgg
    22681 aacgctggct gcttccagcg tatcagcacg ttttcgccaa cgtctttaat ggcaatctgg
    22741 cgaaatacga tccctggcat gcggcacatc gtacggaagt tgaagagtac acggtggaca
    22801 acaccaccaa atgttccgtg tttcggacat tccagggctg gactgcgctc tcggatatgc
    22861 tgcctggtca ggggttgctg cacgtcgtgc ccattcctga agccatggcg tacgtactgt
    22921 tacgtccgct gcttgatgat gtgccggagg atgaactgtg cggcgtagcg cccggaagag
    22981 tgttgccggt atcagagcaa tggcatccac tgttaattga ggcgttaacc agcattccaa
    23041 aactcgaggc cggagactcc gtctggtggc actgcgacgt catccattcc gttgcccccg
    23101 ttgaaaatca acagggttgg ggcaacgtga tgtacattcc tgcggcaccg atgtgcgaga
    23161 aaaatcttgc ctacgcgcac aaggtgaagg ccgcactgga aaaaggcgca tcgccgggcg
    23221 acttcccgcg cgaggactat gaaacaaact gggaaggacg ctttacgctt gccgacctca
    23281 acattcacgg taagcgagcg ttgggcatgg atgtttgaga gttaatggaa gtgaattgat
    23341 gtgggattaa gaaaatcgat tcgacgttac tcagattgct gacaagtgcg ctttcttaat
    23401 gcctgatgcg gcgttaacgc cttatccggc ctacaaagtc ctgcagattc aatatattac
    23461 aggtactgcg taggcctgat aagcgtagcg catcaggcta ttttgcattt gtcatcagcc
    23521 gtcgtcaccc cgacagtatc ggggtgacgg ataatcactt actcgatccc tttactacgc
    23581 aggtaatctt cgtaattacc gctaaagtcg atcacgcgtt ccggggtgat ttccagaatg
    23641 cgggtcgcca gggagcttac gaactcacgg tcgtgtgaaa caaagatcag cgtgccctga
    23701 tacagttcca gcgccatgtt cagtgactcg atggattcca tatccaggtg gttggtcggt
    23761 tcgtccatga tcagaatgtt cggcttctgc atcattaact taccaaacag catccgccct
    23821 ttttccccac cggaaagcac tttagctggc tttttgatgt cgtcctggct gaacagcaaa
    23881 cgaccgagaa tgctgcgtac cgcctgctcg tcatcgcctt cctgcttcca ctgactcatc
    23941 cattcgaaca cggtcagatc attttcaaac tcatattcgt ggtcctgggc gtagtaacca
    24001 atgcgcgcgt tttcagacca ttttacggtg ccgctgtccg gttgcagatc gcccaccagc
    24061 gttttcagca gcgttgattt accgacgccg ttggtaccca gtaccgcaag tttttcaccg
    24121 acctccagca gcaggttgag atttttaaac agtggaccgt tatcaaaccc tttggtcaga
    24181 ccttccactt ccagcgcgtt acggaacagt ttcttatcct gttcaaaacg gataaacggg
    24241 ttctgacggc tggaggcttt cacctcttcc agtttgattt tatcaatctg gcgcgcgcgc
    24301 gaagttgcct ggcgagattt cgaggcgttg gcgctaaagc ggctaacgaa agattgcaac
    24361 tcagcaatct gcgctttctt cttggcgtta tcggccagca gacgttcacg cgcttgggtc
    24421 gccgccgtca tgtactcatc gtagttaccc ggataaacgc gcagctcgcc gtaatccaga
    24481 tccgccatgt gggtacagac catgttaagg aagtgacggt cgtgcgagat gatgatcatg
    24541 gtgctgtcac gctcgttcag cacctgttcc agccagcgaa tggtgtcgat gtcgaggttg
    24601 ttggtcggtt cgtcgagcag gagaatatcc ggctcagcaa acagcgcctg cgccagaagc
    24661 acacgcagct tccagccagg agcaacttca ctcatcgggc cgtagtgctg ttccactgga
    24721 attcccacgc caagcaacag ttcaccggcg cgagcttccg cagagtaacc gtccatttcg
    24781 ccgtatttca cttccagatc ggccacttta tagccgtctt cttcactcat ttccggcaaa
    24841 gcatagatgc ggtcgcgctc ctgcttcact tcccacaact ctttatgccc catgatcacc
    24901 gtatccagca cagtgaactc ttcaaaggca aactgatcct gacgcagctt accaatgcgc
    24961 tcgttgggat cgagggaaac gttacccagc gtcggctcaa ggtcgccgcc gagaatcttc
    25021 ataaaggtgg atttaccact accgttcgcg ccaatcaggc cgtaacggtt gccgccgccg
    25081 aatttgacgg aaatgttttc aaacaacggc ttactgccga actgcatggt gacgttactg
    25141 gaaactaaca caggcgtatc ctgaaaagag atatgacaaa ccgcgcatta tgccataagt
    25201 ctgaaagaag atcctgctcc tcgcttaagg tctacactca agccaaaggc aaaaaatgtg
    25261 atttcgtaca catctgattt cactgtgagc tggaatgaac ttataatgcg cttccaatac
    25321 tctaatattc tcaacccaat ggcctgccag gcacaaaatc tcgcttaaca tgaatatgaa
    25381 attgaaaaca ttattcgcag cggccttcgc tgttgtcggc ttttgcagta ccgcctctgc
    25441 ggtaacttat cctctgccaa ccgacgggag tcgcctggtt ggtcagaatc aggtgatcac
    25501 cattcctgaa ggtaacactc agccgctgga gtattttgcc gcggagtacc agatggggct
    25561 ttccaatatg atggaagcga acccgggtgt ggataccttc ctgccgaaag gcggtactgt
    25621 actgaacatt ccgcagcagc tgatcctgcc ggataccgtt catgaaggca tcgtcattaa
    25681 cagtgcagag atgcgtctgt attactatcc gaaagggacc aacaccgtta tcgtgctgcc
    25741 gatcggcatt ggtcagttag gcaaagatac gcctatcaac tggaccacca aagttgagca
    25801 taagaaagca ggcccgacct ggacgccgac cgccaaaatg cacgcagagt accgcgctgc
    25861 gggcgaaccg ctaccggctg tcgttccggc aggtccggat aacccgatgg gtctgtatgc
    25921 actctacatc ggtcgcctgt atgctatcca tggcaccaac gccaacttcg gtatcggcct
    25981 gcgtgtaagt catggttgtg tgcgtctgcg taacgaagac atcaaattcc tgttcgagaa
    26041 agtaccggtc ggtacccgcg tacagtttat tgatgagccg gtaaaagcga ccaccgagcc
    26101 agacggcagc cgttatattg aagtccataa cccgctgtct accaccgaag cccagtttga
    26161 aggtcaggaa atcgtgccaa ttaccctgac gaagagcgtg cagacagtga ccggtcagcc
    26221 agatgttgac caggttgttc ttgatgaagc gatcaaaaat cgttccggga tgccggttcg
    26281 cctgaattaa tcgtcaaaac gtaaaacaaa ggcggactca taatccgcct tttttatttg
    26341 ccagacctta ccaggccggg atcataacta acaaaacgta tcccaccagc gccgcccaca
    26401 acagcatcgg tattgaatag agatggaaac gccaccagat gcggcgatcg ttcgccatac
    26461 gtagcgcaat caaatttgcc agcgatcctg gtaataaacc aaagcccccc acgtttaccg
    26521 cccatgccag taataaagac ggaggtacat aattgagcag caaaatggta ctcggcacgt
    26581 tactgatcac ctgcgataaa ccgactgccg ttaaccataa cccgggttca gataaatgac
    26641 tcacgttacc caacacgcct tgcaacgctg gaagctgggt cagtaaatgg acgtcgataa
    26701 acatcgccat aaacaccagc agcaacgtcc agtccacact caacaccacg cgacgcgcca
    26761 gcagcacaaa gcccgccgcc acaatcacca gtccccacag ctcttgtttg aactccagcg
    26821 ccgtcagaaa gacgatatac agccccagac aattccacac cagccgcggt ttccactccg
    26881 gtgtttgcac ccccgtatgg tattgcagcg cctttccagg gaaacaacac cagcacaaca
    26941 gcaggagtgt cagcatcatt gcgccagcca tcggtgccat ttgggcaata aatccggcaa
    27001 acgaaagacc agaacgtccc cagataagaa tattttgcgg gttgccaatt ggcgtcagta
    27061 gcgaaccagc gttgactgcc agcgcctcaa aaataatcag ccgattaacc gggatctcac
    27121 acagtctttt tagcgtgata gtcagcggaa caacaataaa cagcgcgaca tcgttggtca
    27181 gaaaggtaga aagcagcgcc gccgccagca ccataaacat cgccagccga cgctccgtag
    27241 caaagcggcg caccattttg cgccccagca catcaaaata accgcttaac tccacacctt
    27301 tggtcagcag catcaggccg cttaaggtga tgatggtgtg ccagtcgata gcagcaggcc
    27361 aggatttcgg tgcaaagggc acgaaaaagc ttaatccgat accaacaaga attaataact
    27421 gaaaaaaacg atcgccttgc agcgtgcgta aaaaaggcag gctcatttgg caccgtgttt
    27481 ctgggtaaac aaacgaaaag cgtcgagcgt ctcttcacta acatggtgct ccatgccttc
    27541 cgcgtcgcga cgggcgattt ccggactgac gcccaacacc aacaagaaat tttcgactat
    27601 ctgatgtcgc tcgcggcttt cctgcgccag cttctctcct tctgccgtta aaaacacgcc
    27661 acgccagggg atcatttcaa tcagccccat ggttgccagc cgcttaagca ttttagccac
    27721 cgtcggttgc gaaactccca gacgagcagc catgtccacc tgacgagctt cccccacttc
    27781 cctgatcaag tcagaaatca gctcaacgta atcatcaata agctcgcgcc gatgcgcctc
    27841 tctgacctgg cggaaccctt caacgtgctc ttccacgttc actaactgcg tcactttttt
    27901 tgctattggc gtacctgcgc gacgactcat tgtgcttcct cattatggtg acgcatacat
    27961 agcgttctca tttcgtgcgc acattgtaaa ccagagttgc gaaggtacaa aaaattaacg
    28021 ttttagcaat agctatataa tatagcctgt actatatctg tatgtaatgc aatcatccct
    28081 caaggatcga cgggattagc aagtcaggag gtcttatgaa tgagttcaag aggtgtatgc
    28141 gcgtgtttag tcattctccc tttaaagtac ggttaatgct gctctctatg ttgtgcgata
    28201 tggtcaacaa caaaccgcag caagataaac cttcagataa atagcggcgt cgcggtacgc
    28261 cgcttcactc ctgcttttat gcaggcataa cgcgttttgg tctgaaaaaa accacttttt
    28321 gtcggatttg caatcccctt cgcaaaagat ttgttcgtca gtagttgacc tgaacggcgg
    28381 ctcgctctat cttcttgcag ccctgcgtat attgcggctc gcggatgcgg acccctttcc
    28441 cctcttcacg cactcttgca ggtattgacc cttgacgcca gggtaagcac atggcgtttg
    28501 ttacgatagt ggcatatgaa tttaaccctc aaagaatcgc ttgttacccg tagccgggta
    28561 tttagcccgt ggactgcgtt ctacttttta cagtcgctat taattaacct cggcttaggt
    28621 taccccttca gtttgctcta caccgctgcg tttacggcta ttttgctttt gctatggcga
    28681 acattgcctc gcgtacaaaa agttctggtc ggtgtcagtt cgctggtggc ggcttgttat
    28741 ttcccttttg ctcaggccta cggcgcgcct aatttcaata cattgctggc attgcactcc
    28801 accaatatgg aagagtcgac cgaaatcctg acgatttttc cgtggtacag ctacctggtc
    28861 ggcttattta tttttgcgct cggcgtaata gcaatcaggc gaaaaaaaga gaatgaaaaa
    28921 gcgcgctgga ataccttcga cagcctgtgt ctggtattca gtgtggcgac attttttgtt
    28981 gctcccgtgc aaaacctggc ctggggtggc gtatttaaac tgaaagatac tggctatccg
    29041 gtatttcgtt ttgctaagga tgtcatcgtc aataataacg aggtgattga agagcaagaa
    29101 cggatggcaa aactttccgg aatgaaagat acctggacgg tcactgccgt taagccgaag
    29161 tatcagacct atgtggtagt gatcggtgaa agcgcgcgtc gcgatgccct cggtgccttt
    29221 ggcggtcact gggacaatac cccgtttgcc agcagcgtta acggtttgat atttgctgac
    29281 tacattgctg ccagtggctc cacgcagaaa tcgcttggct taacgctcaa tcgcgtagtc
    29341 gatggcaaac cacagtttca ggataacttt gtcaccctgg caaatcgcgc gggcttccag
    29401 acctggtggt tttccaacca gggccaaatc ggcgaatacg atactgctat cgccagcatc
    29461 gccaaacgtg cagatgaagt gtacttcctg aaagaaggta attttgaagc agataaaaac
    29521 acgaaagacg aagcgttact ggatatgacc gctcaagtgc tggcgcaaga gcactcgcaa
    29581 ccgcagctga ttgttctgca tctgatgggc tcgcatccgc aggcctgcga caggacacaa
    29641 ggcaaatacg aaacctttgt gcaatcgaaa gaaacgtcgt gctatctcta taccatgacg
    29701 caaacggacg atttactgcg caagctgtac gatcagttac gcaacagcgg cagcagcttc
    29761 tctctggttt acttttctga ccacggtctg gcctttaaag agcgcggcaa agacgtgcaa
    29821 taccttgccc atgatgataa atatcagcaa aatttccagg tgccttttat ggtcatttcc
    29881 agcggcgata aagcgcatcg ggtgattaaa gcccgccgct cagccaatga cttcttaggc
    29941 tttttctcgc agtggacggg aattaaagcg aaggaaataa atatcaaata cccgtttata
    30001 tctgagaaga aagccgggcc gatatacatc accaacttcc agttacagaa ggtggattac
    30061 aaccatctcg gaaccgatat tttcgacccg aaaccttaaa aacaaaaatc cgccccgaga
    30121 ggcggatttt tatatcacca aagtgattag aagcggtaac caacaccggc aatccaggtg
    30181 cctacgtcaa cgctacgaat acggctctgc tcgtaagaga agtccagagc aacgttttcc
    30241 atcgggttga actgcagacc agcaccgtag gagaaaccgt agtcgctggt gtcgtgtttg
    30301 taggtcgggt attcagtggt ctggaattta ccataaccca cacccactac accgtagatg
    30361 cttgcccagt cgttaatgcg gtaagccgga ccagcagtga tgccgtagta ctggtttttg
    30421 ttgtagtcac cagagcttgc agtacggctt ttctcggtgt aagtgaaaga accgatcaca
    30481 cccagcgggc tgttgtcttc ttcatagcgg tatttcaggt tgaaaccgcc cattttgttc
    30541 atttggccct gagcgtcgct ctgtgcgtaa ccgccagtta cggtagaagt cgcagctacg
    30601 gaagtacctg cggtgaaagc cagaactgcg gccagtgctg aaagacatgc aatttttttc
    30661 ataaccacct caaatgtgat tcaaataagt cctaagtttt aaatatatca aaaattaatg
    30721 ggaaactctt cgcgatttgt gatgtctaac gggccatttc atgtaacaga acgtttccat
    30781 acaccgctat ccatctaaat ttaaatcact ttttcagaga actgcgtaag tattacgcat
    30841 gttttccctg tcattcatcc agattattcc taatcaccag actaatgatt ccatcaatcc
    30901 tggcgcattt tagtcaaaac gggggaaaat tttttcaaca aatgctcaac cagcattggg
    30961 tatatccagt acactccacg ctttacttaa gtctagatat ttgtgggaga aaggatgcct
    31021 ggttcattac gtaaaatgcc ggtctggtta ccaatagtca tattgctcgt tgccatggcg
    31081 tctattcagg gtggagcctc gttagctaag tcactttttc ctctggtgag cgcaccgggt
    31141 gtcactgcgc tgcgtctggc attaggaacg ctgatcctca tcgcgttctt taagccatgg
    31201 cgactgcgct ttgccaaaga gcaacggtta ccgctgttgt tttacggcgt ttcgctgggt
    31261 gggatgaatt atctttttta tctttctatt cagacagtac cgctgggtat tgcggtggcg
    31321 ctggagttca ccggaccact ggcggtggcg ctgttctctt ctcgtcgccc ggtagatttc
    31381 gtctgggttg tgctggcggt tcttggtctg tggttcctgc taccgctggg gcaagacgtt
    31441 tcccatgtcg atttaaccgg ctgtgcgctg gcactggggg ccggggcttg ttgggctatt
    31501 tacattttaa gtgggcaacg cgcaggagcg gaacatggcc ctgcgacggt agcaattggt
    31561 tcgttgattg cagcgttaat tttcgtgcca attggagcgc ttcaggctgg tgaagcactc
    31621 tggcactggt cggttattcc attgggtctg gctgtcgtta ttctctcgac cgctctgcct
    31681 tattcgctgg aaatgattgc cctcacccgt ttgccaacac ggacatttgg tacgctgatg
    31741 agcatggaac cggcgctggc tgccgtttcc gggatgattt tcctcggaga aacactgaca
    31801 cccatacagc tgctggcgct cggcgctatc atcgccgctt caatggggtc tacgctgaca
    31861 gtacgcaaag agagcaaaat aaaagaatta gacattaatt aaatttacat ttctgtatgg
    31921 ttatgcataa ccatgcagaa tttctcgcta cttttcctct acaccgtatt tatatatcga
    31981 attatgcaaa agcatattta ttccgaaaat tcctggcgag cagataaata agaattgttc
    32041 ttatcaatat atctaactca ttgaatcttt attagttttg tttttcatgc ttgttaccac
    32101 tattagtgtg ataggaacag ccagaatagc ggaacacata gccggtgcta tacttagtct
    32161 cgttaattac tgggacataa catcaagagg atatgaaatt atgagtaccg ctaaattagt
    32221 taaatcaaaa gcgaccaatc tgctttatac ccgcaacgat gtctccgaca gcgagaaaaa
    32281 agcaacagta gagttgctga atcgccaggt tatccagttt attgatcttt ctttgattac
    32341 caaacaagcg cactggaaca tgcgcggcgc taacttcatt gccgtacatg aaatgctgga
    32401 tggcttccgc accgcactga tcgatcatct ggataccatg gcagaacgtg ccgtgcagct
    32461 gggcggtgta gctctgggga ccactcaagt tatcaacagc aaaaccccgc tgaaaagtta
    32521 cccgctggac atccacaacg ttcaggatca cctgaaagaa ctggctgacc gttacgcaat
    32581 cgtcgctaat gacgtacgca aagcgattgg cgaagcgaaa gatgacgaca ccgcagatat
    32641 cctgaccgcc gcgtctcgcg acctggataa attcctgtgg tttatcgagt ctaacatcga
    32701 ataaatccat cgctgatggt gcagaacttt agtacccgat aaaagcggct tcctgacagg
    32761 aggccgtttt gttttgcagc ccacctcaac gcacttattt agtgcatcca tctgctatcc
    32821 ccagctgatt aagtaaattt tttgtatcca catcatcaca caatcgttac ataaagattg
    32881 ttttttcatc aggttttacg ctaaataatc actgtgttga gtgcacaatt ttagcgcacc
    32941 agattggtgc cccagaatgg tgcatcttca gggtattgcc ctataaatcg tgcatcacgt
    33001 ttttgccgca tctcgaaaaa tcaatgagtt gcaaaattgg cacgattttt tcatatatgt
    33061 gaatgtcacg caggggatcg tcccgtggat agaaaaaagg aaatgctatg aagtctgtat
    33121 taaaagtttc actggctgca ctgaccctgg cttttgcggt ttcttctcat gccgcggata
    33181 aaaaattagt tgtcgcgacg gataccgcct tcgttccgtt tgaatttaaa cagggcgata
    33241 aatatgtggg ctttgacgtt gatctgtggg ctgccatcgc taaagagctg aagctggatt
    33301 acgaactgaa gccgatggat ttcagtggga tcattccggc actgcaaacc aaaaacgtcg
    33361 atctggcgct ggcgggcatt accatcaccg acgagcgtaa aaaagcgatc gatttctctg
    33421 acggctacta caaaagcggc ctgttagtga tggtgaaagc taacaataac gatgtgaaaa
    33481 gcgtgaaaga tctcgacggg aaagtggttg ctgtgaagag cggtactggc tccgttgatt
    33541 acgcgaaagc aaacatcaaa actaaagatc tgcgtcagtt cccgaacatc gataacgcct
    33601 atatggaact gggcaccaac cgcgcagacg ccgttctgca cgatacgcca aacattctgt
    33661 acttcatcaa aaccgccggt aacggtcagt tcaaagcggt aggtgactct ctggaagcgc
    33721 agcaatacgg tattgcgttc ccgaaaggta gcgacgagct gcgtgacaaa gtcaacggcg
    33781 cgttgaaaac cctgcgcgag aacggaactt acaacgaaat ctacaaaaaa tggttcagta
    33841 ctgaaccgaa ataataacgc tacacctgta aagcgcactg gcagttccct ctcccccctg
    33901 gggagaggat tagggtgagg ggcgcaaacc cgctccgggg ccattaatta ccctgaattt
    33961 gattatttac accacggtaa caggaacaac atatgcagtt tgactggagt gccatctggc
    34021 ctgccattcc gcttctgatt gaaggtgcca aaatgaccct gtggatttcg gtcctcggtc
    34081 tggctggcgg tctggtaatc ggattgctgg caggttttgc acgcaccttc ggtggttgga
    34141 tagccaacca cgtcgcgctg gtctttattg aagtgatccg cggcacacct atcgtcgtcc
    34201 aggtgatgtt tatctatttc gccctgccga tggcgtttaa cgacttacgc atcgacccat
    34261 ttactgcggc ggtggtcacc atcatgatca actccggcgc gtacattgcg gaaatcacgc
    34321 gtggtgcggt gctgtctatc cacaaaggtt ttcgtgaagc aggactggcg ctcggtcttt
    34381 cacgttggga aaccattcgc tacgtcattt taccgctggc actgcgtcgt atgctgccgc
    34441 cgctgggtaa ccagtggatc atcagcatta aagacacctc gctgtttatt gttatcggcg
    34501 tggcggaact gacccgtcag gggcaagaaa ttattgccgg taacttccgc gcccttgaga
    34561 tctggagcgc cgtggcggtg ttctatctga ttattaccct ggtgctgagc tttattctgc
    34621 gtcgtctgga aagaaggatg aaaatcctgt gattgaattt aaaaacgtct ccaagcactt
    34681 tggcccaacc caggtgctgc acaatatcga tttgaacatt gcccagggcg aagtcgtggt
    34741 gattatcggg ccgtccggtt ccggtaaatc gaccctgctg cgctgcatca acaaactgga
    34801 agaaatcacc tccggcgatc tgattgtcga tggcctgaag gtcaacgatc cgaaagttga
    34861 cgagcgcctg attcgccagg aagcaggtat ggtgttccag cagttttacc tcttcccgca
    34921 tctgacggcg ctgggaaacg tcatgtttgg tccgctacgc gtgcgtggcg cgaacaaaga
    34981 agaggcggaa aaactggcgc gtgagctgct ggcgaaagtc ggtctggcag aacgtgcaca
    35041 tcactaccct tccgaacttt ctggtggtca acagcagcgt gtggcgattg cccgcgcgct
    35101 ggcggtgaag ccgaaaatga tgctgtttga tgaaccgact tccgctcttg acccggaact
    35161 gcgccatgaa gtgctgaagg ttatgcagga tctggctgaa gaagggatga cgatggtgat
    35221 cgtgacccac gaaatcggtt ttgccgagaa agtagcttcg cggctgatct ttatcgacaa
    35281 aggccggatt gcggaagatg gcaatccgca ggtgttgatc aagaacccgc cgagccagcg
    35341 cttgcaggaa tttttgcagc acgtctctta ataagacaca ttgcctgatg cgctacgctt
    35401 atcaggccta caggatatct ggcaacttat taaaattgca tgaacttgta ggacggataa
    35461 ggcgttcacg ccgcatccgg caaaaaagcc cgcacgttgt cagcaacctg cttaatatcc
    35521 cttcctccct ttcacccgaa agggaggcac accagattcc tctcatttaa aatcgcccct
    35581 cccccagcat ctatacttat ctttttgctc tgttttctca ctggaggagt catgcggtgg
    35641 atcctgttca tcctcttctg cctgctgggc gcacctgccc acgcggtatc catacccggc
    35701 gttacaacca caacgacaac ggactcaacg actgaaccgg ccccggaacc ggatatcgaa
    35761 caaaaaaagc ggcctatggc gcactggcgg atgtgctgga taacgacacc tcgcgtaaag
    35821 agttgatcga ccagttgcgt actgtcgccg cgacgccccc tgcggaaccg gtcccgaaga
    35881 tcgtgccgcc gacgctggtt gaagagcaaa ccgtgctgca aaaggtcacc gaagtcagcc
    35941 gccattatgg tgaagccctt tccgcccgct tcgggcaact ttatcgcaat atcaccggct
    36001 ccccgcataa gccatttaat ccacaaacct tcagcaatgc gctgagccat ttttcaatgt
    36061 tagcggtatt agtgtttggt ttttactggc tgattcgcct gtgcgcactg ccgctatatc
    36121 gcaaaatggg tcagtgggcg aggcaaaaaa atcgtgagcg cagcaactgg ttgcagcttc
    36181 cggcgatgat tattggggcg tttattatcg acctgctgtt actggccctg acattatttg
    36241 tcggccaggt attaagcgac aacctgaatg caggcagtcg caccatcgct ttccaacaaa
    36301 gtttgtttct caacgccttt gccctgattg aatttttcaa agccgtacta cgcctgattt
    36361 tttgcccaaa cgtggcggag ctgcgcccgt tcacgattca ggacgagagc gcccgttact
    36421 ggggtcgtcg cctgagctgg ttaagcagcc tgattggcta tggcctgatt gtggccgtgc
    36481 cgattatctc taatcaggtg aatgtacaga taggtgcgct ggcgaacgtc atcattatgc
    36541 tgtgcatgac cgtctgggcg ttgtacctga tcttccgaaa taaaaaagag attacccagc
    36601 atatgctcaa ctttgcggag cattcgctgg cttttttcag cctgtttatc cgcgcctttg
    36661 cgctggtgtg gcactggctg gcaagcgcct attttatcat gctgtttttc ttttcgttgt
    36721 tcgatccggg caacagtctg aaatttatga tgggtgcaac ggtgcgcagc ctggcgatta
    36781 ttggtatcgc agcgtttgtt tccggtatgt tttcccgctg gctggcgaaa accatcaccc
    36841 tctcgccaca tactcagcgt aactatccgg agctacaaaa acggctgaat ggctggcttt
    36901 cggcggcgct gaaaacggcg cgtattctga ccgtctgcgt ggcagtaatg ttgctgttga
    36961 gcgcatgggg attgttcgat ttctggaact ggctgcaaaa cggcgcgggg cagaaaaccg
    37021 tagatatcct gatccgcatc gcactcattc ttttcttctc ggcggttggc tggacggtgc
    37081 tcgccagttt gatcgaaaac cggctggctt cggatattca tggccgcccg ctacccagcg
    37141 cccgtacgcg taccctgctg acgctgttcc gtaacgcgct ggcggtgatt atcagtacca
    37201 tcaccatcat gattgtgttg tcggaaatcg gcgtcaatat cgcgccattg ctggcaggtg
    37261 ccggggcatt aggtctggct atctcgtttg gttcgcaaac gctggtaaaa gatattatca
    37321 ccggggtatt tattcagttt gaaaacggca tgaacactgg agatttggtg actatcgggc
    37381 cgttgaccgg cacagtggaa cggatgtcga ttcgctccgt gggcgtgcgc caggataccg
    37441 gggcgtatca catcattccg tggtcttcga taaccacctt tgctaacttc gtccgcggta
    37501 ttggttcggt agtggcaaac tacgatgttg atcgccatga agatgctgat aaagccaatc
    37561 aggcactgaa agatgcggta acggaattga tggaaaacga agaaattcgc gggctgatta
    37621 ttggtgaacc gaactttgcc gggattgtcg gcttaagcaa taccgcgttt acactgcgtg
    37681 tttcgttcac cacgctgcca ctcaaacagt ggacggtacg ctttgccctc gacagccagg
    37741 tgaaaaaaca tttcgacctg gcgggcgttc gcgcgccagt gcagacttat caggtgctgc
    37801 ctgctccggg cgcgaccccg gctgaaccgc tgccgccggg ggagccaacg ctttaacgtt
    37861 ggcgattaac aaaacggcgg cgctgctcgt cgttcataaa ggtccaggca ataaagcgac
    37921 tctgcttttg cccctgggcc atctcttttt ttaccacctt caccgcgccc acgtcggtca
    37981 gagcacgata caacggcggt aagttttcac cacgagatac cagcgaggta aaccacatca
    38041 cctgcttcgc gaagcctttg ctctcttcaa tcatcttttt gataaagttg acttcaccgc
    38101 cttcacacca caactcctgt tgctgaccac caaagttcag tgcatcgtct ttgttcagcc
    38161 ccaggttacg gcgtttacgc tcactacctg cccgcgccgc agcggcggaa tcgtggaatg
    38221 gcgggttaca caaggtcgcg tcgtattgct cgtttttgtg gatgatgccg ttaaaaatcg
    38281 ccccactctc tttttgccga cgcagacgaa tggcgcggtt aagccccgga ttagcactga
    38341 taatcgcctg cgcactgctt aacgcctggc tgctggtttc actaccggta aaacgccagc
    38401 catattcatg tacgccaatc agcggataaa tacagttcgc accaacgccg atatccagaa
    38461 tgctggcatt cgctggaatt gttccgcttg cttcagccag taaatcggca aggtgatgaa
    38521 tataatccgc ccggcccggt actggtgggc agagaaaacc gtcggggata tcccagttcg
    38581 ctacggcgta aaaatgggcc agcaacgcct tattgagcgc cttcaccgcc agcggattgg
    38641 caaagtctac gctttgctcc cctgcgggtg taagcgtgag gaattgcctg agttcaggat
    38701 tgacctgaca aagcgtggcg agatcgtagc ggctgtgatg acggttgcgc ggatgcaacc
    38761 ccggtttctg ggcggacata gcattctcct ttaagcagcg gcgtaagata cccgcataac
    38821 gcgacagggt aaatatctgg ctgcgtttat cattccccaa aaccttaaca tggctatggt
    38881 ttatggcaat tcatcataaa aatcagcatt atgcatgtca ttgatttgtc ttattttgta
    38941 aaaatatatc gttcaaaaaa taagcatttc tctgtcaatt ttttgcgtca tttgcatcgc
    39001 gcaggcgacc tacacttatc aggcactacc cgttgtattt atgaggatgt cattatgaaa
    39061 aagtgcctca cactactgat tgccaccgtc ctgagcggaa tctctctcac ggcttatgcc
    39121 gcgcaaccga tgagtaacct ggacagcggt cagctacggc ccgccggtac cgtttcggca
    39181 actggcgcat caaacctaag cgatctggag gataaactgg cagaaaaagc gcgcgaacaa
    39241 ggcgcgaaag gttatgtcat taattccgca ggcggaaatg accagatgtt aggtactgca
    39301 accatctaca aataacccca ccaacgtaac tgcattcgcc tctggatgca gttttcgcct
    39361 gttttgtaat caaaaaacat caaatatgaa cgcaatgtaa ataaatgtat ttctttttcg
    39421 cgcaatgggt gatagaaaat cgcatcaagt gataatgctt atcaaaatta ttatcacttg
    39481 cacgaacact atcacgggat taacagtggc atcgcatccg cagagatgct ttctcgtggc
    39541 agtgaaaatt tcaacatata agaaaaagtc acctgcaaaa tggaaaacaa tcgcaatttc
    39601 cctgccagac aatttcattc gctcacgttc tttgccggtc tttgtattgg tattacgcct
    39661 gtggcacagg cactcgccgc cgaagggcaa gctaacgcgg atgacacgct ggttgtcgaa
    39721 gcatcaacgc cttcgcttta tgcgccacaa caatctgccg atccgaaatt ctcgcgtccg
    39781 gtagcggata ctacccgcac gatgacggtg atttctgaac aagtgattaa agatcagggc
    39841 gcaaccaacc ttaccgacgc gctcaaaaac gtccccggcg tg
//
LOCUS       DAJTRC010000028        38363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.028, whole genome shotgun sequence.
ACCESSION   DAJTRC010000028 DAJTRC010000000
VERSION     DAJTRC010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.028"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..248)
                     /gene="yefM"
                     /locus_tag="OO357_001561"
     CDS             complement(<1..248)
                     /gene="yefM"
                     /locus_tag="OO357_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoeB-YefM toxin-antitoxin system antitoxin YefM"
                     /protein_id="HCR5886549.1"
                     /translation="MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEE
                     YNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKDII"
     gene            531..572
                     /gene="hisL"
                     /locus_tag="OO357_001562"
     CDS             531..572
                     /gene="hisL"
                     /locus_tag="OO357_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002231208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="his operon leader peptide"
                     /protein_id="HCR5886550.1"
                     /translation="MTRVQFKHHHHPD"
     misc_feature    548..671
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00514"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="His leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF00514"
     gene            718..1617
                     /gene="hisG"
                     /locus_tag="OO357_001563"
     CDS             718..1617
                     /gene="hisG"
                     /locus_tag="OO357_001563"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HCR5886551.1"
                     /translation="MTDNTRLRIAMQKSGRLSDDSRELLARCGIKINLHTQRLIAMAE
                     NMPIDILRVRDDDIPCLVMDGVVDLGIIGENVLEEELLNRRAQGEDPRYFTLRRLDFG
                     GCRLSLATPVDEAWDGPLSLNGKRIATSYPHLLKRYLDQKGISFKSCLLNGSVEVAPR
                     AGLADAICDLVSTGATLEANGLREVEVIYRSKACLIQRDGEMEESKQQLIDKLLTRIQ
                     GVIQARESKYIMMHAPTERLDEVIALLPGAERPTILPLAGDQQRVAMHMVSSETLFWE
                     TMEKLKALGASSILVLPIEKMME"
     gene            1623..2927
                     /gene="hisD"
                     /locus_tag="OO357_001564"
     CDS             1623..2927
                     /gene="hisD"
                     /locus_tag="OO357_001564"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HCR5886552.1"
                     /translation="MSFNTIIDWNSCTAEQQRQLLMRPAISASESITRTVNDILDNVK
                     TRGDEALREYSAKFDKTTVTALKVSADEIAAASERLSDELKQAMAVAVKSIETFHTAQ
                     KLPPVDVETQLGVRCQQVTRPVASVGLYIPGGSAPLFSTVLMLATPARIAGCKKVVLC
                     SPPPIADEILYAAQLCDVQDVFNVGGAQAIAALAFGTESVPKVDKIFGPGNAFVTEAK
                     RQVSQRLDGAAIDMPAGPSEVLVIADSGATPDFVASDLLSQAEHGPDSQVILLTPDAD
                     MARRVAEAVERQLAELPRAETTRQALNASRLIVTKDLAQCVEISNQYGPEHLIIQTRN
                     ARELVDGITSAGSVFLGDWSPESAGDYASGTNHVLPTYGYTATCSSLGLADFQKRMTV
                     QELSKEGFSALASTIETLAAAERLTAHKNAVTLRVNVLKEQA"
     gene            2924..3994
                     /gene="hisC"
                     /locus_tag="OO357_001565"
     CDS             2924..3994
                     /gene="hisC"
                     /locus_tag="OO357_001565"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HCR5886553.1"
                     /translation="MSTVTITDLARENVRNLTPYQSARRLGGNGDVWLNANEYPTAVE
                     FKLTQQTLNRYPECQPKAVIENYAQYAGVKPEQVLVSRGADEGIELLIRAFCEPGKDA
                     ILYCPPTYGMYSVSAETIGVECRTVPTLENWQLDLQGISDKLDGVKVVYVCSPNNPTG
                     QLINPQDFRTLLELTRGKAIVVADEAYIEFCPQASLAGWLAEYPHLAILRTLSKAFAL
                     AGLRCGFTLANEEVINLLMKVIPPYPLSTPVADIAAQALSPQGIVAMSERVAQIITER
                     EYLIAALKEIPCVEQVFDSETNYILARFKASSAVFKSLWDQGIILRDQNKQPSLSGCL
                     RITVGTREESQRVIDALRAEQV"
     gene            3994..5061
                     /gene="hisB"
                     /locus_tag="OO357_001566"
     CDS             3994..5061
                     /gene="hisB"
                     /locus_tag="OO357_001566"
                     /EC_number="3.1.3.15"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     histidinol-phosphatase/imidazoleglycerol-phosphate
                     dehydratase HisB"
                     /protein_id="HCR5886554.1"
                     /translation="MSQKYLFIDRDGTLISEPPSDFQVDRFDKLAFEPGVIPELLKLQ
                     KAGYKLVMITNQDGLGTQSFPQADFDGPHNLMMQIFTSQGVQFDEVLICPHLPADECD
                     CRKPKVKLVEGYLAEQAMDRANSYVIGDRATDIQLAENMGINGLRYDRETLNWPMIGE
                     QLTKRDRYAHVVRNTKETQIDVQVWLDREGGSKINTGVGFFDHMLDQIATHGGFRMEI
                     NVKGDLYIDDHHTVEDTGLALGEALKIALGDKRGICRFGFVLPMDECLARCALDISGR
                     PHLEYKAEFTYQRVGDLSTEMIEHFFRSLSYTMGVTLHLKTKGKNDHHRVESLFKAFG
                     RTLRQAIRVEGDTLPSSKGVL"
     gene            5061..5651
                     /gene="hisH"
                     /locus_tag="OO357_001567"
     CDS             5061..5651
                     /gene="hisH"
                     /locus_tag="OO357_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HCR5886555.1"
                     /translation="MNVVILDTGCANLNSVKSAIARHGYEPKVSRDPDIVLLADKLFL
                     PGVGTAQAAMDQVRERELFDLIKACTQPVLGICLGMQLLGRRSEESNGVDLLGIIDED
                     VPKMTDFGLPLPHMGWNRVYPQAGNRLFQGIEDGAYFYFVHSYAMPVNPWTIAQCNYG
                     EPFTAAVQKDNFYGVQFHPERSGAAGAKLLKNFLEM"
     gene            5651..6388
                     /gene="hisA"
                     /locus_tag="OO357_001568"
     CDS             5651..6388
                     /gene="hisA"
                     /locus_tag="OO357_001568"
                     /EC_number="5.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-(5-phosphoribosyl)-5-[(5-
                     phosphoribosylamino)methylideneamino]imidazole-4-
                     carboxamide isomerase"
                     /protein_id="HCR5886556.1"
                     /translation="MIIPALDLIDGTVVRLHQGDYGKQRDYGNDPLPRLQDYAAQGAE
                     VLHLVDLTGAKDPAKRQIPLIKTLVAGVNVPVQVGGGVRSEEDVAALLEAGVARVVVG
                     STAVKSPDVVKGWFERFGADALVLALDVRIDEQGNKQVAVSGWQENSGVSLEQLVETY
                     LPVGLKHVLCTDISRDGTLAGSNVSLYEEVCARYPQVAFQSSGGIGDINDVAALRGTG
                     VRGVIVGRALLEGKFTVKEAIACWQNA"
     gene            6370..7146
                     /gene="hisF"
                     /locus_tag="OO357_001569"
     CDS             6370..7146
                     /gene="hisF"
                     /locus_tag="OO357_001569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HCR5886557.1"
                     /translation="MLAKRIIPCLDVRDGQVVKGVQFRNHEIIGDIVPLAKRYAEEGA
                     DELVFYDITASSDGRVVDKSWVSRVAEVIDIPFCVAGGIKSLEDAAKILSFGADKISI
                     NSPALADPTLITRLADRFGVQCIVVGIDTWYDAETGKYHVNQYTGDESRTRVTQWETL
                     DWVEEVQKRGAGEIVLNMMNQDGVCNGYDLKQLKKVREVCHVPLIASGGAGTMEHFLE
                     AFRDADVDGALAASVFHKQIINIGELKAYLATQGVEIRIC"
     gene            7140..7751
                     /locus_tag="OO357_001570"
     CDS             7140..7751
                     /locus_tag="OO357_001570"
                     /EC_number="3.5.4.19"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoribosyl-AMP
                     cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE"
                     /protein_id="HCR5886558.1"
                     /translation="MLTEQQRRELDWEKTDGLMPVIVQHAVSGEVLMLGYMNPEALDK
                     TIESGKVTFFSRTKQRLWIKGETSGNFLNVVSIAPDCDNDTLLVLANPIGPTCHKGTS
                     SCFGNTAHQWLFLYQLEQLLAERKYADPETSYTAKLYASGTKRIAQKVGEEGVETALA
                     ATVHDRFELTNEASDLMYHLLVLLQDQDLDLTTVIENLHKRHQ"
     gene            complement(7937..8914)
                     /gene="wzzB"
                     /locus_tag="OO357_001571"
     CDS             complement(7937..8914)
                     /gene="wzzB"
                     /locus_tag="OO357_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310855.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen chain length determinant protein
                     WzzB"
                     /protein_id="HCR5886559.1"
                     /translation="MRVENNNVSGQNHDPEQIDLIDLLVQLWRGKMTIIISVIVAIAL
                     AIGYLAVAKEKWTSTAIITQPDVGQIAGYNNAMNVIYGQAAPKVSDLQETLIGRFSSA
                     FSALAETLDNQEEPEKLTIEPSVKNQQLPLTVSYVGQTAEGAQMKLAQYIQQVDDKVN
                     QELEKDLKDNIALGRKNLQDSLRTQEVVAQEQKDLRIRQIQEALQYANQAQVTKPQVQ
                     QTEDVTQDTLFLLGSEALESMIKHEATRPLVFSPNYYQTRQNLLDIEKLKFDDLDIHA
                     YRYVMKPTLPIRRDSPKKAITLILAVLLGGMVGAGIVLGRNALRNYNAK"
     gene            complement(9060..10180)
                     /gene="ugd"
                     /locus_tag="OO357_001572"
                     /pseudo
     CDS             complement(9060..10180)
                     /gene="ugd"
                     /locus_tag="OO357_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 6-dehydrogenase"
     gene            complement(10430..11836)
                     /gene="gndA"
                     /locus_tag="OO357_001573"
     CDS             complement(10430..11836)
                     /gene="gndA"
                     /locus_tag="OO357_001573"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HCR5886560.1"
                     /translation="MSKQQIGVVGMAVMGRNLALNIESRGYTVSIFNRSREKTEEVIA
                     ENPGKKLAPYYTVKEFVESLETPRRILLMVKAGAGTDAAIDSLKPYLDKGDIIIDGGN
                     TFFQDTIRRNRELSAEGFNFIGTGVSGGEEGALKGPSIMPGGQKEAYELVAPILTKIA
                     AVAEDGEPCVTYIGADGAGHYVKMVHNGIEYGDMQLIAEAYSLLKGGLNLSNEELAQT
                     FTEWNNGELSSYLIDITKDIFTKKDEDGNYLVDVILDEAANKGTGKWTSQSALDLGEP
                     LSLITESVFARYISSLKDQRVAASKVLSGPQAQSAGDKAEFIEKVRSALYLGKIVSYA
                     QGFSQLRAASEEYNWDLNYGEIAKIFRAGCIIRAQFLQKITDAYAENPQIANLLLAPY
                     FKQIADDYQQALRDVVAYAVQNGIPVPTFAAAVAYYDSYRAAVLPANLIQAQRDYFGA
                     HTYKRIDKEGVFHTEWLD"
     gene            complement(11945..12304)
                     /locus_tag="OO357_001574"
     CDS             complement(11945..12304)
                     /locus_tag="OO357_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001313975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886561.1"
                     /translation="MNFNSRLESRYSYELMKKASEYSELYGDNLIQLGLEDGIYFYKG
                     MAIGDVFGLARYSDWTISNPECEVIPQDDLIEKMKSFNSSFIVISKRSYANFNPEKYP
                     KFKVLMDTPNGILIAIK"
     gene            complement(12301..12807)
                     /locus_tag="OO357_001575"
     CDS             complement(12301..12807)
                     /locus_tag="OO357_001575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886562.1"
                     /translation="MATILGGIYWYVRSFWISGDPFSPAGGNIFGHYLWNEIDLQVQT
                     AEQARHGISPATLDLQQAINKVGSDVVFYAILSVFTLRNNKILWVFFSIVLTYVLFWF
                     SVTQGDRYLSPIYPLSVLQVAISIASLIKDINITNYKLGRYFILVCIIFFRGDRCILN
                     SNIFILNT"
     gene            complement(12953..13516)
                     /locus_tag="OO357_001576"
     CDS             complement(12953..13516)
                     /locus_tag="OO357_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886563.1"
                     /translation="MVGQFNKIAIFLIFTVCWVLSIIKRQQFRWLAINNIEFSTLFVI
                     LFLVLIFVVTLLSSLRAPGDWDDTMYHLPLARSLVEHHAIVVEQYLRFPLFPQNADLL
                     MALGLQLGDVRLAQFLANICFFVIACGLVGCSWEITKTYYPGIIATILLFTINPLKDH
                     LGYAYIDLTLSLFCCSQYSYIYSLRKQ"
     gene            complement(13723..14661)
                     /locus_tag="OO357_001577"
                     /pseudo
     CDS             complement(13723..14661)
                     /locus_tag="OO357_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000274881.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
     gene            complement(14724..15098)
                     /locus_tag="OO357_001578"
     CDS             complement(14724..15098)
                     /locus_tag="OO357_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bactoprenol-linked glucose translocase"
                     /protein_id="HCR5886564.1"
                     /translation="MLKIGKLLTSSFFSYFLIGIVNTALHWGVFYACYNNLAFGQGRS
                     NIVGFICAATFSFFANARCSFKVSATKARYFIFIFFMGAMSYLFGVLFDLLALSPIFT
                     LFTFSLFSLVLGYCASKYFIFR"
     gene            complement(15102..16250)
                     /gene="wzy"
                     /locus_tag="OO357_001579"
     CDS             complement(15102..16250)
                     /gene="wzy"
                     /locus_tag="OO357_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004016722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen polymerase"
                     /protein_id="HCR5886565.1"
                     /translation="MNNINKIFITFLCIELIIGGGGRLLEPLGIFPLRYLLFVFSFIL
                     LIFNLVTFNFSITQKCVSLFIWLLLFPFYGFFVGLLAGNKINDILFDVQPYLFMLSLI
                     YLFTLRYTLKVFSCEIFIKIVNAFALYGSLLYISYIILLNFGLLNFNLIYEHLSLTSE
                     FFFRPDGAFFSKSFYFFGVGAIISFVDKKYLKCLIIVLAILLTESRGVLLFTTLSLLL
                     ASFKLHKLYLNTIIIILGSVLFIIMLYMVGSRSEDSDSVRFNDLYFYYKNVDLATFLF
                     GRGFGSFILDRLRIEIVPLEILQKTGVIGVFISLVPMLLIFLKGYFLNSTKTSLMMSL
                     ILFFSITVSITNPFLFTPMGIFIIGVVVLWVFSIENIQISNNLTSGAK"
     gene            complement(16270..17175)
                     /gene="rfbG"
                     /locus_tag="OO357_001580"
     CDS             complement(16270..17175)
                     /gene="rfbG"
                     /locus_tag="OO357_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-rhamnosyl transferase RfbG"
                     /protein_id="HCR5886566.1"
                     /translation="MHSSDQKRVAVLMATYNGECWIEEQLKSIIEQKDVDISIFISDD
                     LSTDNTLNICEEFQLSYPSIINILPSVNKFGGAGKNFYRLIKDVDLENYDYICFSDQD
                     DIWYKDKIKNAIDCLVFNNANCYSSNVIAYYPSGRKNLVDKAQSQTQFDYFFEAAGPG
                     CTYVIKKETLIEFKKFIINNKNAAQDICLHDWFLYSFARTRNYSWYIDRKPTMLYRQH
                     ENNQVGANISFKAKYKRLGLVRNKWYRKEVTKIANALADDSFVNNQLGKGYIGNLILA
                     LSFWKLRRKKADKIYILLMLILNIF"
     gene            complement(17168..18058)
                     /locus_tag="OO357_001581"
     CDS             complement(17168..18058)
                     /locus_tag="OO357_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnosyltransferase"
                     /protein_id="HCR5886567.1"
                     /translation="MNSNIYAVIVTYNPELKNLNALITELKEQNCYVVVVDNRTNFTL
                     KDKLADIEKVHLICLGRNEGIAKAQNIGIRYSLEKGAEKIIFFDQDSRIRNEFIKKLS
                     CYMDNENAKIAGPVFIDRDKSHYYPICNIKKNGLREKIHVTEGQTPFKSSVTISSGTM
                     VSKEVFEIVGMMDEELFIDYVDTEWCLRCLNYGILVHIIPDIEMVHAIGDKSVKICGI
                     NIPIHSPVRRYYRVRNAFLLLRKNHVPLLLSIREVVFSLIHTTLIIATQKNKIEYMKK
                     HILATLDGIRGITGGGRYNA"
     gene            complement(18088..19344)
                     /gene="wzx"
                     /locus_tag="OO357_001582"
     CDS             complement(18088..19344)
                     /gene="wzx"
                     /locus_tag="OO357_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O13/O129/O135 family O-antigen flippase"
                     /protein_id="HCR5886568.1"
                     /translation="MSIIKNSVWNLFGYAIPTLIAIPSLGFLARGLGPEGFGVYTIAI
                     ALVGYAGIFDVGLTRSVIREIAIHRDNHHERTKVISTSTSFLVLFSCFGAFLLLIFSD
                     GIVNYLKISGVEHSDIQLAFKLLAICIPLFILNQLWSAILEGDEKFGIVNIQKSISSS
                     CIAGIPAIFVFYSATLSAAVAGLIFARVISILVSAYYVRNDIKISGVHFCYKTFKRLF
                     FFGGWMTVSNIISPVMVYFDRFIVSNIMGADKVAFYSAPAEVILKLGIIPAAIGRAVF
                     PRLSNIKDFKEFKRNVNKSLLLMFLICLPVIIIGLLYSGLVLKIWFGENYQINSFNIL
                     NVLLIGFFFNALAMIPFSAIQALGKSKITALIHCAELVPYLALLYFMVEKYGLLGAAI
                     SWSIRVILDALLLQWLYTRMCSVYEN"
     gene            complement(19360..19905)
                     /gene="rfbC"
                     /locus_tag="OO357_001583"
     CDS             complement(19360..19905)
                     /gene="rfbC"
                     /locus_tag="OO357_001583"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HCR5886569.1"
                     /translation="MNVIKTEIPDVLIFEPKVFGDERGFFMESFNQKVFEEAVGRKVE
                     FVQDNHSKSTKGVLRGLHYQLEPYAQGKLVRCVVGEVFDVAVDIRKSSPTFGKWVGVN
                     LSAENKRQLWIPEGFAHGFCVLSDEAEFVYKTNNFYSKMQERGILWSDKSINIEWPVQ
                     NPLLSDKDINGQKFVDADYFI"
     gene            complement(19910..20788)
                     /gene="rfbA"
                     /locus_tag="OO357_001584"
     CDS             complement(19910..20788)
                     /gene="rfbA"
                     /locus_tag="OO357_001584"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5886570.1"
                     /translation="MKTRKGIILAGGSGTRLYPVTMAVSKQLLPIYDKPMIYYPLSTL
                     MLAGIRDILIISTPQDTPRFQQLLGDGSQWGLNLQYKVQPSPDGLAQAFIIGEEFIGG
                     DDCALVLGDNIFYGHDLPKLMDTAVNRESGATVFAYHVNDPERYGVVEFDDNGTAISL
                     EEKPQEPKSNYAVTGLYFYDNDVVEMAKNLKPSARGELEITDINRIYMDQGRLSVAMM
                     GRGYAWLDTGTHQSLIEASNFIATIEERQGLKVSCPEEIAHRKGFIDAEQVKVLAEPL
                     KKNAYGQYLLKMIKGY"
     gene            complement(20847..21746)
                     /gene="rfbD"
                     /locus_tag="OO357_001585"
     CDS             complement(20847..21746)
                     /gene="rfbD"
                     /locus_tag="OO357_001585"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HCR5886571.1"
                     /translation="MNILLFGKTGQVGWELQRALAPLGNLIALDVHSTDYCGDFSNPE
                     GVAETVKKIRPDVIVNAAAHTAVDKAESEPNFAQLLNATCVEAIAKAANEVGAWVIHY
                     STDYVFPGNGDTPWLETDATAPLNVYGETKLAGEKALQEHCAKHLIFRTSWVYAGKGN
                     NFAKTMLRLAKEREELAVINDQFGAPTGAELLADCTAHAIRVAANKPEVAGLYHLVAG
                     GTTTWHDYAALVFEEARRAGINLALNKLNAVPTTAYPTPARRPHNSRLNTEKFQQNFA
                     LVLPDWQVGVKRMLNELFTTTAI"
     gene            complement(21746..22831)
                     /gene="rfbB"
                     /locus_tag="OO357_001586"
     CDS             complement(21746..22831)
                     /gene="rfbB"
                     /locus_tag="OO357_001586"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5886572.1"
                     /translation="MKILVTGGAGFIGSAVVRHIINNTQDSVVNVDKLTYAGNLESLA
                     DVSDSERYAFEHADICDAVAMSRIFAQHQPDAVMHLAAESHVDRSITGPAAFIETNIV
                     GTYVLLEAARNYWSALNDEKKKSFRFHHISTDEVYGDLPHPDEANNNEALPLFTETTA
                     YAPSSPYSASKASSDHLVRAWKRTYGLPTIVTNCSNNYGPYHFPEKLIPLVILNALEG
                     KALPIYGKGDQIRDWLYVEDHARALYTVVTEGKAGETYNIGGHNEKKNIDVVLTICDL
                     LDEIVPKEKSYREQITYVADRPGHDRRYAIDADKISRELGWKPQETFESGIRKTVEWY
                     LANTNWVENVKSGTYQSWIEQNYEGRQ"
     gene            complement(23204..24097)
                     /gene="galF"
                     /locus_tag="OO357_001587"
     CDS             complement(23204..24097)
                     /gene="galF"
                     /locus_tag="OO357_001587"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalF"
                     /protein_id="HCR5886573.1"
                     /translation="MTNLKAVIPVAGLGMHMLPATKAIPKEMLPIVDKPMIQYIVDEI
                     VAAGIKEILLVTHASKNAVENHFDTSYELESLLEQRVKRQLLAEVQSICPPGVIIMNV
                     RQGEPLGLGHSILCARPAIGDNPFVVVLPDVVIDDASADPLRYNLAAMIARFNETGRS
                     QVLAKRMPGDLSEYSVIQTKEPLDREGKVSRIVEFIEKPDQPQTLDSDIMAVGRYVLS
                     ADIWPELERTQPGAWGRIQLTDAIAELAKKQSVDAMLMTGDSYDCGKKMGYMQAFVKY
                     GLRNLKEGAKFRKGIEKLLSE"
     gene            complement(24272..25666)
                     /gene="wcaM"
                     /locus_tag="OO357_001588"
     CDS             complement(24272..25666)
                     /gene="wcaM"
                     /locus_tag="OO357_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis protein WcaM"
                     /protein_id="HCR5886574.1"
                     /translation="MPFKTLSRRTFLTASSALAFLHTPFARALPARQSVNINDYNPHD
                     WIASFKQAFSEGQTVVVPAGFVCDNINTGIFIPPGKTLHILGSLRGNGRGRFVLQDGS
                     QVTGGEGGGMHNITLDVRGSDCTIKGLAMSGFGPVMQIYIGGKNKRVMRNLTIDNLTV
                     SHANYAILRQGFHNQIIGANITNCKFSDLQGDAIEWNVAINDSDILISDHVIERINCT
                     NGKINWGIGIGLAGSTYDNNYPEDQAVKNFVVANITGSDCRQLIHVENGKHFVIRNIN
                     ARNITPDFSKKAGIDNATVAIYGCDNFVIDNIEMINSAGMLIGYGVIKGKYLSIPQNF
                     RVNNIQLDNTHLAYKLRGIQISAGNAVSFVSLTNIEMKRASLELHNKPQHLFMRNINV
                     MQESSVGPALSMNFDMRKDVRGVFMAKKETLLSLANVHAVNERGQSSVDIDRINHHIV
                     NVEKINFRLPERRE"
     gene            complement(25677..26897)
                     /gene="wcaL"
                     /locus_tag="OO357_001589"
     CDS             complement(25677..26897)
                     /gene="wcaL"
                     /locus_tag="OO357_001589"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaL"
                     /protein_id="HCR5886575.1"
                     /translation="MKVGFFLLKFPLSSETFVLNQITAFIDMGFEVEIVALQKGDTEN
                     THAAWTKYNLVARTRWLQDEPAGKVAKLRHRASQTLRGIHRKNTWHALNLKRYGAESR
                     NLILSAICGQVATPFRADVFIAHFGPAGVTAAKLRELGIIRGKIATIFHGIDISSREV
                     LNHYTPEYQQLFRRGDLMLPISDLWAGRLQKMGCPREKIAVSRMGVDMTRFSPRPVKA
                     PATPLEIISVARLTEKKGLHVAIEACRQLKEQGVAFRYRILGIGLWERRLRTIIEQYQ
                     LEDVVEMPGFKPSHEVKEMLDDADVFLLPSVTGADGDMEGIPVALMEAMAVGIPVVST
                     LHSGIPELVEADKSGWLLPENDARALAQRLAAFSQLDTDELAPVVKRACEKVEHDFNQ
                     QVINRELASLLQAL"
     gene            complement(26894..28174)
                     /gene="wcaK"
                     /locus_tag="OO357_001590"
     CDS             complement(26894..28174)
                     /gene="wcaK"
                     /locus_tag="OO357_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis pyruvyl transferase
                     WcaK"
                     /protein_id="HCR5886576.1"
                     /translation="MKLLILGNHTCGNRGDSAILRGLLDAINILNPHTEVDVMSRYPV
                     SSSWLLNRPVMGDPLFLQMTQHNSAAGVVGRIKKVLRRRYQHQVLLSRVTDTGKLRNI
                     AIAQGFTDFVRLLSGYDAIIQVGGSFFVDLYGVPQFEHALCTFMAKKPLFMIGHSVGP
                     FQDEQFNQLANYVFGHCDALILRESVSLDLMKRRNITTAKVEHGVDTAWLVDHHTEDF
                     TASYAVQHWLDVAAQQKTVAITLRELAPFDKRLGTTQQAYEKAFAGVVNRILDEGYQV
                     IALSTCTGIDSYNKDDRMVALNLRQHISDPARYHVVMDELNDLEMGKILGACELTVGT
                     RLHSAIISMNFATPAIAINYEHKSAGIMQQLGLPEMAIDIRHLLDGSLQAMVADTLGQ
                     LPVLNARFNEAVSRERQTGMQMVQSVLERIGEVK"
     gene            complement(28354..29832)
                     /gene="wzxC"
                     /locus_tag="OO357_001591"
     CDS             complement(28354..29832)
                     /gene="wzxC"
                     /locus_tag="OO357_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid undecaprenyl disphosphate flippase
                     WzxC"
                     /protein_id="HCR5886577.1"
                     /translation="MSLREKTISGAKWSAIATVIIIGLGLVQMTVLARIIDNHQFGLL
                     TVSLVIIALADTLSDFGIANSIIQRKEISHLELTTLYWLNVGLGLVVCVAVFLLSDVI
                     GDVLNNPDLAPLIKTLSLAFVVIPHGQQFRALMQKELEFNKIGMIETSAVLAGFTFTV
                     VSAHFWPLAMTAILGYLVNSAVRTLLFGYFGRKIYCPGLHFSLASVAPNLRFGAWLTA
                     DSIINYLNTNLSTLVLARILGAGVAGGYNLAYNVAVVPPMKLNPIITRVLFPAFAKIQ
                     DDTEKLRVNFYKLLSIVGIINFPALLGLMVVSNNFVPLVFGEKWNSIIPVLQLLCIVG
                     LLRSVGNPIGSLLMAKARVDISFKFNVFKTFLFIPAIVIGGQMAGAIGVTLGFLLVQI
                     INTILSYFVMIKPVLGSSYRQYILSLWLPFYLSLPTLVVSYALGIVLKGQLALGMLLA
                     VQIAAGVLAFVVMIVLSRHPLVVEVKRQFCRSEKMKMLLRAG"
     gene            complement(29834..31228)
                     /gene="wcaJ"
                     /locus_tag="OO357_001592"
     CDS             complement(29834..31228)
                     /gene="wcaJ"
                     /locus_tag="OO357_001592"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HCR5886578.1"
                     /translation="MTNLKKRERAKTNASLISMVQRFSDITIMFAGLWLVCEVSGLSF
                     LYMHLLVALITLVVFQMLGGITDFYRSWRGVRAATEFALLLQNWTLSVIFSAGLVAFN
                     NDFDTQLKIWLAWYGLTSIGLVVCRSCIRIGAGWLRNHGYNKRMVAVAGDLAAGQMLM
                     ESFRNQPWLGFEVVGVYHDPKPGGVSNDWAGNLQQLVEDAKAGKIHNVYIAMQMCDGA
                     RVKKLVHQLADTTCSVLLIPDVFTFNILHSRLEEMNGVPVVPLYDTPLSGVNRLLKRA
                     EDIVLATLILLLISPVLCCIALAVKLSSPGPVIFRQTRYGMDGKPIKVWKFRSMKVME
                     NDKVVTQATQNDPRVTKVGNFLRRTSLDELPQFINVLTGGMSIVGPRPHAVAHNEQYR
                     QLIEGYMLRHKVKPGITGWAQINGWRGETDTLEKMEKRVEFDLEYIREWSVWFDIKIV
                     FLTVFKGFVNKAAY"
     gene            complement(31283..32653)
                     /gene="cpsG"
                     /locus_tag="OO357_001593"
                     /gene_synonym="manB"
     CDS             complement(31283..32653)
                     /gene="cpsG"
                     /locus_tag="OO357_001593"
                     /gene_synonym="manB"
                     /EC_number="5.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707943.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis phosphomannomutase
                     CpsG"
                     /protein_id="HCR5886579.1"
                     /translation="MKKLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLG
                     GDVRLTSETLKLALAKGLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPM
                     DYNGMKLVREGARTISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
                     GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIP
                     NPLLPECRDDTRNAVIKHGADMGIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLE
                     KNPGAKIIHDPRLSWNTIDVVTAAGGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHY
                     FRDFAYCDSGMIPWLLVAELVCLKGKTLGELVRDRMAAFPASGEINSKLAQPVEAINR
                     VEQHFSREALAVDRSDGISMTFADWRFNLRTSNTEPVVRLNVESRGDVPLMEARTRTL
                     LTLLNE"
     gene            complement(32846..34282)
                     /gene="cpsB"
                     /locus_tag="OO357_001594"
     CDS             complement(32846..34282)
                     /gene="cpsB"
                     /locus_tag="OO357_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanyltransferase"
                     /protein_id="HCR5886580.1"
                     /translation="MAQSKLYPVVMAGGSGSRLWPLSRVLYPKQFLCLKGDLTMLQTT
                     ICRLNGVECESPVVICNEQHRFIVAEQLRQLNKLTENIILEPAGRNTAPAIALAALAA
                     KRHSPESDPLMLVLAADHVIADEDAFRAAVRNAMPYAEAGKLVTFGIVPDLPETGYGY
                     IRRGEVSVGEQDTVAFEVAQFVEKPNLETAQAYVASGEYYWNSGMFLFRAGRYLEELK
                     KYRPDILDACEKAMSAVDPDLDFIRVDEEAFLACPEESVDYAVMEPTADAVVVPMDAG
                     WSDVGSWSSLWEISAHTAEGNVCHGDVINHKTENSYVYAESGLVTTVGVKDLVVVQTK
                     DAVLIADRNAVQDVKKVVEQIKADGRHEHRVHREVYRPWGKYDSIDAGDRYQVKRITV
                     KPGEGLSVQMHHHRAEHWVVVAGTAKVTIDGDIKLLGENESIYIPLGATHCLENPGKI
                     PLDLIEVRSGSYLEEDDVVRFADRYGRV"
     gene            complement(34285..35508)
                     /gene="wcaI"
                     /locus_tag="OO357_001595"
     CDS             complement(34285..35508)
                     /gene="wcaI"
                     /locus_tag="OO357_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis fucosyltransferase
                     WcaI"
                     /protein_id="HCR5886581.1"
                     /translation="MKILVYGINYSPELTGIGKYTGEMVEWLAAQGHEVRVITAPPYY
                     PQWQVGENYSAWRYKREEGAATVWRCPLYVPKQPSTLKRLLHLGSFAVSSFFPLMAQR
                     RWKPDRIIGVVPTLFCTPGMRLLAKLSGARTVLHIQDYEVDAMLGLGLAGKGKGGKVA
                     QLATAFERSGLHNVDNVSTISRSMMNKAIEKGVAADNVIFFPNWSEIARFQHVADADV
                     DALRNQLGLPDNKKIILYSGNIGEKQGLENVIEAADLLRDEPLIFAIVGQGGGKARLE
                     KMAQQRGLRNMQFFPLQSYDALPALLKMGDCHLVVQKRGAADAVLPSKLTNILAVGGN
                     AVITAEAHTELGQLCETFPGIAVCVEPESVEALVAGIRQALLLPKHNTVAREYAERTL
                     DKENVLRQFINDIRG"
     gene            complement(35505..35984)
                     /gene="gmm"
                     /locus_tag="OO357_001596"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
     CDS             complement(35505..35984)
                     /gene="gmm"
                     /locus_tag="OO357_001596"
                     /gene_synonym="nudD"
                     /gene_synonym="wcaH"
                     /gene_synonym="yefC"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707946.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose mannosyl hydrolase"
                     /protein_id="HCR5886582.1"
                     /translation="MFLRQEDFATVVRSTPLVSLDFIVENSRGEFLLGKRTNRPAQGY
                     WFVPGGRVQKDETLEAAFERLTMAELGLRLPITAGQFYGVWQHFYDDNFSGTDFTTHY
                     VVLGFRFRVAEEELLLPDEQHDDYRWLTPDALLASNDVHANSRAYFLAEKRAGVPGL"
     gene            complement(35984..36952)
                     /gene="fcl"
                     /locus_tag="OO357_001597"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
     CDS             complement(35984..36952)
                     /gene="fcl"
                     /locus_tag="OO357_001597"
                     /gene_synonym="wcaG"
                     /gene_synonym="yefB"
                     /EC_number="1.1.1.271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HCR5886583.1"
                     /translation="MSKQRIFIAGHCGMVGSAIKRQLEQRGDVELVLRTRDELNLLDS
                     RAVHDFFASERIDQVYLAAAKVGGIVANNTYPADFIYQNMMIESNIIHAAHQNDVNKL
                     MFLGSSCIYPKLAKQPMAESELLQGTLEPTNEPYAIAKIAGIKLCESYNRQYGRDYRT
                     VMPTNLYGPHDNFHPSNSHVIPALLRRFHEATAQNAPDVVVWGSGTPMREFLHVDDMA
                     AASIHVMELAHEVWLENTQPMLSHINVGTGVDCTIRELAQTIAKVVGYKGRVVFDASK
                     PDGTPRKLLDVTRLHQLGWYHEISLEAGLASTYQWFLENQDRFRGE"
     gene            complement(36955..38076)
                     /gene="gmd"
                     /locus_tag="OO357_001598"
     CDS             complement(36955..38076)
                     /gene="gmd"
                     /locus_tag="OO357_001598"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HCR5886584.1"
                     /translation="MSKVALITGVTGQDGSYLAEFLLEKGYEVHGIKRRASSFNTERV
                     DHIYQDPHTCNPKFHLHYGDLSDTSNLTRILREVQPDEVYNLGAMSHVAVSFESPEYT
                     ADVDAMGTLRLLEAIRFLGLEKKTRFYQASTSELYGLVQEIPQKETTPFYPRSPYAVA
                     KLYAYWITVNYRESYGMYACNGILFNHESPRRGETFVTRKITRAIANIAQGLESCLYL
                     GNMDSLRDWGHAKDYVKMQWMMLQQEQPEDFVIATGVQYSVRQFVEMAAAQLGIKLRF
                     EGTGVEEKGIVVSVTGHDAPGVKPGDVIIAVDPRYFRPAEVETLLGDPTKAHEKLGWK
                     PEITLREMVSEMVANDLEAAKKHSLLKSHGYDVAIALES"
     gene            complement(38102..>38363)
                     /locus_tag="OO357_001599"
     CDS             complement(38102..>38363)
                     /locus_tag="OO357_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaF"
                     /protein_id="HCR5886585.1"
                     /translation="HSVISQKSYLCTGSHDHASQHFTINATPIVIGEKCWLATDVFVA
                     PGVTIGDGTVVGARSSVFKSLPANVVCRGNPAVVIRERVETE"
BASE COUNT        10243 a         9611 c         8645 g         9864 t
ORIGIN      
        1 tcaataatgt ccttttccgt tcctttgcct gatttcaggc tatcgattga gtccatcaat
       61 ctccgggcgt tagcggggga acgcagtaga taagccgtct cttccagcga attgtattct
      121 tcgagtgaca tcagaacaca agcctctcca ttctgacgag taatgaggat cggggcatga
      181 tcttcaacgg ctttcatcat tgttgccgac aaattctgac gcgcttcgct gtagctaatt
      241 gtacgcatgt aaatctccta ttttgtacag ttcattgtac aatgataggt gttaattaac
      301 tatttattaa ttagtttgta gatcaagaca ttgccagcga gacgaaaaat cagactttgc
      361 tctttttggt gctgtcaagt tagaggacag tcctcttagc cccctccttt ccccgctcat
      421 tcattaaaca aatccatcgc cataaaatat ataaaaaagc ccttgctttc taacgtgaaa
      481 gtggtttagg ttaaaagaca tcagttgaat aaacattcac agagattttt atgacacgcg
      541 ttcaatttaa acaccaccat catcctgact agtctttcag gcgatgtgtg ctggaagaca
      601 ttcagatctt ccagtggtgc atgaacgcat gagaaagccc ccggaagatc accttccggg
      661 ggctttttta ttgcgcggtt gttaacggtt cagacaggtt taaagaggaa taagaaaatg
      721 acagacaaca ctcgtttacg catagctatg cagaaatccg gccgtttaag tgatgactca
      781 cgcgaattac tggcgcgctg tggcattaaa atcaatcttc acacccagcg cctgatcgcg
      841 atggcagaaa acatgccgat tgatattctg cgcgtgcgtg acgatgacat tccctgtctg
      901 gtaatggacg gcgtggtaga ccttgggatt atcggcgaaa acgtgctgga agaagagctg
      961 cttaaccgcc gcgcccaggg tgaagatccg cgctacttta ccctgcgtcg tctggatttc
     1021 ggcggctgcc gcctttcgct ggcaacgccg gttgatgaag cctgggacgg tccgctctcc
     1081 ttaaacggta aacgtatcgc cacctcttat cctcacctgc tcaagcgtta tctcgaccag
     1141 aaaggtatct cttttaaatc ctgcttactg aacggttctg tcgaagttgc gccgcgtgcc
     1201 gggctggcgg atgcgatttg cgatctggtt tccaccggtg ccacgctgga agctaacggc
     1261 ctgcgcgaag tcgaagttat ctaccgctcg aaagcctgcc tgatccagcg cgatggcgaa
     1321 atggaagaat ctaaacagca actgatcgac aagctactga cccgtattca gggtgtgatc
     1381 caggcgcgcg aatcaaaata catcatgatg cacgcaccga ccgaacgcct ggatgaagtc
     1441 atcgccctgc tgccaggtgc cgaacgccca accattctgc cgctggcggg tgatcaacag
     1501 cgcgtagcga tgcacatggt gagtagcgaa accctgttct gggaaacgat ggaaaaactg
     1561 aaagcactgg gtgccagttc aatcctggtc ctgccgattg agaagatgat ggagtgatcg
     1621 ccatgagctt taacacaatc attgactgga atagctgtac tgcggagcaa caacgccagc
     1681 tgttaatgcg cccggcgatc tccgcttctg aaagcattac ccgcaccgtt aacgatattc
     1741 tcgataacgt gaaaacgcgt ggcgatgagg ccctgcgtga atacagcgcg aagtttgata
     1801 aaaccacggt taccgcgctg aaggtgtctg ctgatgagat cgccgccgcc agcgaacgcc
     1861 tgagcgacga gctaaaacag gcgatggcgg tggcagtaaa gagtattgaa accttccaca
     1921 ctgcgcaaaa actgccgccg gtagatgtag aaacgcagct aggcgtgcgt tgccagcagg
     1981 tcacgcgccc ggtggcttca gtgggtttgt atattccggg cggctccgcc ccgctcttct
     2041 caacggtatt aatgctggca actccggcgc gtattgcggg ctgtaaaaaa gtggtgctgt
     2101 gctcaccgcc gccgattgcc gatgaaattc tttatgcggc gcagctgtgc gatgtgcagg
     2161 acgtgtttaa cgtcggcggc gcacaggcca ttgccgcgct ggcgtttggt acggaatctg
     2221 tgccgaaagt ggacaaaatc ttcgggccgg gtaacgcctt tgtcaccgaa gcaaaacgcc
     2281 aggtaagcca gcgtctggac ggtgcggcga tcgatatgcc cgcaggcccg tcggaagtgc
     2341 tggtgattgc tgacagcggc gctacgccgg attttgtggc ttctgatttg ctttctcagg
     2401 ctgaacacgg ccctgactca caggtaattt tactgacccc cgatgctgat atggcgcgtc
     2461 gcgttgccga ggccgtcgaa cgccaactgg cagaactgcc gcgtgccgaa accacccgcc
     2521 aggcactgaa cgccagccgc ctgatcgtga ctaaagattt agcgcagtgc gtggagatct
     2581 ccaaccagta cggcccggag cacctgatca ttcagacccg caacgcccgc gaactggtcg
     2641 atggcatcac cagcgccggt tcggtatttc ttggtgactg gtcaccggaa tcggcaggcg
     2701 actacgcctc cggcaccaac cacgtgctgc cgacttacgg ttacaccgcc acctgttcca
     2761 gcctcgggct ggcagatttc cagaaacgca tgaccgtaca ggaactgtcg aaagaaggtt
     2821 tctccgcgct ggcttcgacc attgaaacac tggccgccgc cgagcgcctg accgcccaca
     2881 aaaatgccgt tactttgcgt gttaacgtcc ttaaggagca agcatgagca ccgtgactat
     2941 tactgattta gcgcgtgaaa acgtccgcaa cctgacaccg tatcagtcgg cgcgtcgtct
     3001 gggcggtaac ggcgacgtct ggctgaacgc caacgaatat cccacagccg tggagtttaa
     3061 gcttactcag caaacgctca accgctaccc ggaatgccag ccaaaagcgg tgattgaaaa
     3121 ttacgcgcaa tatgcaggcg taaaaccgga acaggtgctg gtcagccgtg gcgcggacga
     3181 aggtattgaa ctgctgatcc gcgctttttg cgaaccgggt aaagacgcca tcctctactg
     3241 cccgccaacg tacggcatgt acagcgtcag cgctgaaacc attggtgtcg aatgccgcac
     3301 agtgccgacg ctggaaaact ggcaactgga cttacagggc atttccgaca agctggacgg
     3361 cgtaaaagtg gtctatgttt gcagccccaa caaccccacc gggcaactga tcaatccgca
     3421 ggattttcgc accctgctgg agctaacccg cggtaaggcg attgtggttg ccgatgaagc
     3481 ttatatcgag ttttgcccgc aggcatcgtt agctggctgg ctggcggaat atccgcacct
     3541 ggcgattttg cgtacccttt cgaaagcctt cgcgttggcg gggcttcgtt gcggatttac
     3601 gctggcaaac gaagaagtca tcaacctgct gatgaaagtg atccccccct acccgctctc
     3661 gacgccggtt gccgacattg ccgcacaggc gttaagcccg cagggaatcg tcgccatgag
     3721 cgaacgggtg gcgcaaatta ttacagaacg cgaatacctg attgccgcac tgaaagagat
     3781 cccctgcgtg gagcaggttt tcgactctga aaccaactac attctggcgc gctttaaagc
     3841 ctccagtgca gtgtttaaat ctttgtggga tcagggcatt atcttacgtg atcagaataa
     3901 acaaccctct ttaagcggct gcctgcgaat taccgtcgga acccgtgaag aaagccagcg
     3961 cgtcattgac gccttacgtg cggagcaagt ttgatgagtc agaagtatct ttttatcgat
     4021 cgcgatggaa ccctgattag cgaaccgccg agtgattttc aggtggaccg ttttgacaaa
     4081 ctcgcctttg aaccgggcgt gatcccggaa ttgctgaagc tgcaaaaagc gggctacaag
     4141 ctggtgatga tcactaatca ggatggtctg ggaacacaaa gtttcccgca agcggatttt
     4201 gatggcccgc acaacctgat gatgcagatc ttcacctcgc aaggcgtgca gtttgatgaa
     4261 gtgctgattt gtccgcacct gcccgccgat gagtgcgact gccgtaagcc gaaagtaaaa
     4321 ctggtggagg gttatctggc tgagcaagcg atggatcgtg ccaacagtta tgtaattggc
     4381 gatcgcgcga ccgacattca actggcggaa aacatgggca ttaatggttt acgctacgac
     4441 cgcgaaaccc tgaactggcc aatgattggc gagcaactca ctaaacgaga ccgttacgcc
     4501 catgtagtgc gcaacaccaa agagacgcag attgacgtcc aggtgtggct ggatcgcgaa
     4561 ggtggcagca agattaacac cggcgttggc ttctttgatc acatgctgga tcagatcgcc
     4621 acccacggcg gtttccgcat ggaaatcaat gtcaaaggcg acctctatat cgacgatcac
     4681 cacaccgtcg aagataccgg cctggcgctg ggcgaagcgt taaaaattgc cctcggcgac
     4741 aaacgcggta tttgccgctt tggttttgtg ctgccgatgg acgaatgcct tgcccgctgc
     4801 gcactggata tctctggtcg cccgcacctg gaatataaag ccgagtttac ctaccagcgt
     4861 gtgggcgatc tcagcactga aatgatcgaa cacttcttcc gttcgctctc ttacaccatg
     4921 ggcgtgacac tacacctgaa aaccaaaggt aaaaacgatc atcaccgtgt agaaagcctg
     4981 ttcaaagcct ttggtcgtac tctgcgccag gccatccgcg tcgaaggcga caccctgccc
     5041 tcgtcgaaag gagtgctgta atgaacgtgg tgatccttga tactggctgc gccaacctga
     5101 actctgtgaa gtctgccatt gcgcgccacg gttatgaacc caaagtcagc cgtgacccgg
     5161 acattgtgtt gctggccgat aaactgtttt tacccggcgt cggcaccgca caagcagcga
     5221 tggatcaggt gcgtgagcgc gagctgtttg atctcatcaa agcctgtacc caaccggtgc
     5281 tgggcatctg cttagggatg caactcctgg gacgacgcag cgaagagagc aatggcgtcg
     5341 acttgctggg catcatcgac gaagacgtgc cgaaaatgac cgactttggt ctgccactgc
     5401 cacatatggg ctggaaccgc gtttatccgc aggcaggcaa ccgcctgttt caggggattg
     5461 aagacggcgc gtacttttac tttgttcaca gctacgcaat gccagtcaat ccgtggacca
     5521 tcgcccagtg taattacggc gaaccgttca ccgcggcggt acaaaaagat aacttctacg
     5581 gcgtgcagtt ccacccggag cgttctggtg ccgctggcgc taagttgctg aaaaacttcc
     5641 tggagatgtg atgattattc cggcattaga tttaatcgac ggcaccgtgg tgcgtctcca
     5701 tcagggcgat tacggcaaac aacgcgatta cggtaacgac ccgctgccgc gcttacagga
     5761 ttacgctgcg caaggtgccg aagtgttgca cctggtggat ctgaccgggg caaaagatcc
     5821 ggctaagcgt caaatcccgc tgattaaaac cctggtcgcg ggcgttaacg tcccggtgca
     5881 ggttggcggc ggtgttcgta gtgaagaaga cgtggcggcg ttactggaag ctggcgttgc
     5941 gcgcgtggtg gtcggttcca ccgcagtgaa atcaccggac gtagtgaaag gctggtttga
     6001 acgtttcggt gccgatgcgc tggtgctggc actggatgtc cgtattgacg agcaaggcaa
     6061 caagcaagtg gcagtcagcg gctggcaaga gaactcgggc gtttcactgg aacaactggt
     6121 ggaaacctat ctgcccgtcg gcctgaaaca tgtgttgtgt accgatatct cgcgcgacgg
     6181 cacgctggcg ggatctaacg tctctttata tgaagaagtg tgtgccagat atccgcaagt
     6241 ggcgtttcag tcctccggcg gcattggcga cattaacgat gttgctgccc tacgtggcac
     6301 tggcgtgcgc ggcgtaatag ttggtcgtgc attactggaa ggtaaattca ccgtgaagga
     6361 ggccatcgca tgctggcaaa acgcataatc ccgtgtctcg acgttcgtga tggtcaggtg
     6421 gtgaaaggcg tacagtttcg caaccatgaa atcattggcg atatcgtgcc actggcaaaa
     6481 cgctacgccg aagaaggtgc agatgaactg gtgttctacg atatcaccgc ttccagcgat
     6541 ggccgtgtgg tagataaaag ctgggtatct cgcgtggcgg aagtgatcga cattccgttt
     6601 tgtgtggcgg gtgggattaa gtctctggaa gatgccgcga aaattctttc ctttggcgcg
     6661 gataaaattt ccatcaactc ccctgcgctg gcagacccaa cattaattac tcgcctggcc
     6721 gatcgctttg gcgtgcagtg tattgtggtt ggtattgata cctggtacga cgccgaaacc
     6781 ggtaaatatc atgtaaatca atataccggc gatgaaagcc gcacccgcgt cactcaatgg
     6841 gaaacgctcg actgggtaga ggaggtgcaa aaacgcggcg ccggagaaat cgtcctcaac
     6901 atgatgaatc aggacggcgt gtgtaacggt tacgacctca agcagctgaa aaaagtgcgt
     6961 gaagtttgcc acgtcccgct gattgcctcc ggtggcgcgg gcaccatgga acacttcctg
     7021 gaagccttcc gcgatgccga cgttgacggt gcgctggcag cttccgtatt ccacaaacaa
     7081 ataatcaata ttggcgaatt aaaagcgtac ctggcaacac agggcgtgga gatcaggata
     7141 tgttaacaga acaacaacgt cgcgaactgg actgggaaaa aaccgacggt ctgatgccgg
     7201 tgattgtgca acacgcggta tctggcgaag tgttaatgct gggctacatg aacccggaag
     7261 ccttagacaa aaccatcgaa agcggtaaag tcaccttctt ctcgcgcact aaacagcgac
     7321 tgtggatcaa aggcgaaacc tcgggcaatt ttctcaatgt ggtaagtatt gccccggact
     7381 gcgacaacga cacattactg gtgctggcga atcccatcgg gccaacctgc cacaaaggca
     7441 ccagtagctg cttcggcaac accgctcacc agtggctgtt cctgtatcaa ctggaacaac
     7501 tgctcgccga acgtaaatat gccgacccgg aaacctccta caccgccaaa ctgtatgcca
     7561 gcggcactaa acgcattgcg cagaaagtgg gcgaagaagg cgtggaaacc gcactggcag
     7621 caacggtaca tgatcgcttt gagctgacca acgaggcatc tgacttgatg tatcacctgc
     7681 tggtgctgtt gcaggatcag gatctggatt taacgacagt aattgagaac ctgcataaac
     7741 ggcatcagtg agttacgggc tgagcaggtg tgatgttgtt gtcacacccg gagccattgc
     7801 ccgatgcgca aacttatcgt gcctacacat tatctcaaca cacgtaggcc ggataagatg
     7861 cgccagcatc gcatccggca aaaaaaacgg gcatggtgtc accaccctgc ccgtttctct
     7921 taaatgcaca ataatattac ttcgcgttgt aattacgcag agcgttacgc cccaacacaa
     7981 tccccgcgcc aaccatgccg cccagaagca ctgccagaat caaggtgatt gcctttttcg
     8041 gactatcgcg acgaataggt aacgtcggtt tcatcacata gcggtaagca tgaatatcaa
     8101 gatcatcaaa ctttaatttt tcaatatcca acaggttttg acgtgtctga tagtagtttg
     8161 gtgagaacac caacggacga gtcgcttcat gcttaatcat cgactccagc gcttcgctcc
     8221 ctagaaggaa caacgtatct tgcgtcacat cttcagtctg ctgaacctgt ggctttgtca
     8281 cctgcgcctg attcgcatac tgcaacgctt cctgaatctg acggatacgc agatctttct
     8341 gctcctgcgc gaccacttcc tgggttctta aagagtcctg caagtttttc cgtcccagag
     8401 caatgttgtc cttgagatcc ttttctagct cttgattcac tttatcatca acttgctgaa
     8461 tgtattgggc caacttcatt tgtgcgccct ctgcagtttg cccaacataa gaaacagtca
     8521 atggtaattg ctggttctta acagaaggtt cgatggtaag tttttctggc tcttcctgat
     8581 tatccagcgt ttctgctaat gcagagaagg cagaactgaa gcgaccaatt aacgtctcct
     8641 gcaaatccga tactttcggt gcagcctgac catagataac attcatggca ttgttatagc
     8701 cagcaatttg ccccacgtcg ggctgagtga taattgctgt tgacgtccat ttctccttcg
     8761 ctactgccaa ataaccaata gccagggcaa tagccacaat gacggaaatg ataattgtca
     8821 tcttgccacg ccacaactgc actagtaaat caatcaaatc aatctgttcc gggtcatggt
     8881 tttgcccaga aacattatta ttttctactc tcatcattac cctaactgac ggaaatttcc
     8941 aagtccgcat agtttagcga taatccccgt gaatgctata ggataagtga taaaaataat
     9001 gaattattgc ggaggtaaca aaagacaatc agggcgtaaa tagccctgat aacaagatgt
     9061 tagtcgctgc caaagagatc gcgggtgtag accttatctg ccacatcctt aagctcttct
     9121 gccatacggt tggagataat gacgtcggct tgttgtttga aggtggcgag atcacgttcc
     9181 aggcgagagt tgaagaatga gtcttctttc atcaccggct cgtagatgat cacttcaacg
     9241 cctttcgcct tgatacgctt cataatcccc tgaatagaag acgcacggaa gttatctgac
     9301 ccgctcttca taatcagacg ataaataccc accacttgcg gtttgcatcg acaattgccg
     9361 agatcaggtt attcggcaca gactggtagt tcgccagtaa ctgcttggta tctttcggca
     9421 gacaataacc accataacca aacgacggat tgttgtaatg gttgccaata cgcggatcga
     9481 gacaaacgcc ttcgataatc tggcgagtat tcagacctaa actttctgca taactatcca
     9541 gttcattaaa gtatgctacg cgcatcgcca gataggtatt agcgaaaagt ttaatcgctt
     9601 ctgcttcagt ggagtcggta aacagggtcg ggatattttg cttaatcgcg ccttcctgta
     9661 acaacgcagc aaaacgttct gcgcgttctg aacgctcacc gatgacaata cgtgacggat
     9721 ggagattatc gtaaagggct ttaccttcgc ggaggaattc cggagagaaa ataatatttt
     9781 cagtacgata tttcttatgc atcgctgcgg taaaaccaac gggaaccgtt gatttgatga
     9841 ccataaccgc ataaggattt atctcaacta cgtctttaat tactgattct acactggatg
     9901 tattgaaata attagtttta ggatcatagt cggttggagt ggcgatgatg acataatcag
     9961 catcccggta ggcttcattt ttatctaatg tggcattaaa gtgtatttta tctgattgca
    10021 aaaactgctg aatttcctta tcaacaatag gagatatccg atcattcagc atagcaacgc
    10081 gtgacggtaa aatatctaat gccacaacct catgattttg tgcgattaga agcccgtttg
    10141 acaagcctac atagccagta ccggaaatgg tgattttcat ttcatgctct cagaattaac
    10201 ttaactgtga atcatgatgt ttttagcatc gtgataagat gctaaaagtt ttaacgctac
    10261 gttgtcagat ggtcaacgca acgaaaaaca attcagattt tctttataag aatagctgat
    10321 gtttattaag ttaatattaa gcagtgaaat ttagtctata agatatttgg caaaaaaaag
    10381 cccggtgcaa tatacgccgg gcctcaattt tattgttggt taaatcagat taatccagcc
    10441 attcagtatg gaacacacct tctttatcaa tgcgcttata agtatgcgcc ccgaaatagt
    10501 cacgctgtgc ctggatcagg ttcgcaggca gaaccgcggc acggtagcta tcgtaatagg
    10561 caaccgcagc ggcgaaggtc ggaaccggga taccgttctg tactgcataa gcgacgacat
    10621 cgcgcagcgc ctgctggtag tcatcggcaa tttgcttgaa gtacggggcc agcagcaggt
    10681 tagcgatctg cggattttcg gcataagcat cggtgatttt ctgcaggaac tgcgcacgga
    10741 tgatgcagcc agcacggaaa atcttcgcga tttcgccgta gttcagatcc caattgtatt
    10801 cttcagacgc agcacgcagc tgagagaagc cctgagcgta agaaacgatt ttgcccagat
    10861 acagcgcact gcgaactttt tcgatgaact cagccttgtc gcctgctgac tgagcttgcg
    10921 gaccagagag aactttagat gcggcaacgc gctgatcttt cagagaagag atataacgtg
    10981 caaacacaga ctcggtaatc agcgacagcg gttcgccgag atccagcgcg ctctggctgg
    11041 tccatttacc ggtgcctttg tttgctgctt catccagaat cacatcaacc aggtagttac
    11101 cgtcttcatc ttttttggtg aagatatctt tagtgatgtc gatcaggtag ctgctaagtt
    11161 caccgttatt ccactcggtg aaagtctggg ccagttcttc attagataga ttcaggccac
    11221 ctttgagcag agaatacgct tcagcaatga gctgcatatc tccgtattca ataccattgt
    11281 gaaccatctt cacataatgg cctgcgccgt cggcaccaat ataagtaacg catggctcgc
    11341 cgtcttctgc cacggcagcg attttagtca ggataggcgc aaccagctca taagcctctt
    11401 tctgcccgcc aggcataata gaaggacctt tcagcgcgcc ctcttcaccg ccggaaacac
    11461 cggtaccgat gaagttaaag ccctctgctg aaagctcacg attacgacga atggtgtcct
    11521 ggaagaaggt attaccacca tcaatgatga tgtcaccttt atcgagatat ggtttcaggg
    11581 aatcaatcgc agcatccgtg cctgcacctg ctttcaccat taacaggatg cgacgaggcg
    11641 tttccagaga ttcaacaaac tctttcaccg tatagtaagg agccagtttc ttgcctggat
    11701 tttcagcaat cacttcttcc gtcttttcgc gggagcggtt gaaaatagag acggtataac
    11761 cacgactttc gatgttgagc gcaaggttgc gccccatcac tgccataccg actacgccga
    11821 tctgttgctt tgacattgtt tactcctgtc ttctatatta ttaaccgtct attaaccgtt
    11881 atattattaa ccgtcgtgat tttcatcttc taatcaagga atacatatgg ttacagcatg
    11941 cttcttactt tatagcgata agtatgccat tgggtgtatc cataagaact ttgaattttg
    12001 gatacttttc tggattaaaa ttagcatagc ttcgtttaga tataacaata aacgaactat
    12061 taaaagattt catcttctct attaaatcat cctgtgggat tacctcacac tcgggattac
    12121 taatagtcca atcactatat cttgctaatc caaagacgtc accaattgcc atccctttat
    12181 agaagtaaat accatcctcc aagcctaatt ggataagatt atctccatat aactcactat
    12241 attcacttgc tttcttcatc agttcatagc tatagcgact ttctaagcgt gaattaaaat
    12301 tcaagtattc agaataaaaa tatttgaatt caaaatacaa cgatcgcccc gaaaaaaaat
    12361 aatacacaca aggataaaat atcgtcccaa cttgtaattt gtaatgttga tatcttttat
    12421 taaacttgca attgagatcg caacctgcaa tactgacaat gggtaaatgg gtgataaata
    12481 cctgtcacct tgcgtaacag aaaaccaaaa taagacatat gttaaaacaa tcgagaaaaa
    12541 tacccaaagt attttattat tcctaagtgt aaagacagac agtatagcat aaaaaacaac
    12601 atcactgcca actttattaa ttgcttgttg caaatctaat gtggcaggac ttataccatg
    12661 tcgagcttgt tctgctgttt gaacttgtag atcaatttca ttccataggt agtgaccaaa
    12721 tatatttcct ccagcaggac taaaaggatc accagagatc caaaaactac ggacatacca
    12781 ataaatgcca ccaagaattg ttgccaagct aaaaataaga aatgtctgta tgtttctctt
    12841 tttcgctatc aatataacaa atatgatggc tccatatatg gcaccaaata gttttacgcc
    12901 acaagcaatt ccaataaaaa aaccacataa aaaaattaat tcattttgtg ttttattgtt
    12961 ttctgagcga atatatataa gaatactggc tgcagcaaaa caaagatagc gttaaatcaa
    13021 tatatgcata accaagatga tctttaagtg gattgatagt aaatagtagg attgtagcaa
    13081 taatgcctgg ataataagtt ttcgtaattt cccatgaaca gccaacaagt ccacaggcta
    13141 ttacaaaaaa acagatattt gctaaaaact gagctaacct tacatctcct aactgtaaac
    13201 ctaaagccat taaaagatct gcattttgcg ggaacagagg aaaacgcaaa tattgttcaa
    13261 caacaattgc atggtgctca actaatgaac gagccaaagg taagtgatac atagtatcat
    13321 cccaatcacc gggagctcgc agggacgata acaatgtaac cacaaaaatc aaaacaagaa
    13381 ataaaataac aaacagcgta ctgaactcaa tattatttat tgccagccat ctaaattgct
    13441 gacgtttaat aatgcttaaa acccaacaca ccgtaaaaat taaaaagata gcaatcttat
    13501 taaattgccc gaccatgccc agaaagtaaa ttatcaaaat atttaaagac atccctatgc
    13561 ataggcaata aaaaataaag aaaatttgtt aagtcctgcc accccctcct taaaaaaaga
    13621 tgacaataca atattaccaa tgacaaaaaa tgtcattcca aaagcaaaac aatacacaac
    13681 atacaaatac aacggcaatt caatcatagt aaaatactct atttacatta tcagtttata
    13741 gtttttttca gaaagataaa ataatgcctt catttcacgt ctagttgttg tatttgaatg
    13801 caaaataatg ccacatacaa atgccattat tgacgataac atgatggagg cagttaaaaa
    13861 ggcagtcgga aatcttctca ccaaacctgt atcaatataa tcaacaatga tgggcaatcc
    13921 taaaattata gacaaagcag caagtaaaac gccgataata ctaaaaaagt atagtggccg
    13981 ttcaagcgag taaagtttaa ttattgtttt cagaatcttg aacccatcac tccaagttga
    14041 tagtttgctc acactccctt cagatcgctc gccatatttt gtattaacct caccatactt
    14101 catacgtaat tctaaagcat gaattgtaag ctcagtttcg atttcaaatc cccttgatag
    14161 acatggaaaa gatttaacat agcgtctgga aaatacacga taccccgaca acatatcagt
    14221 aaaattccct ttaaagatag tctgaacagt accagtcaac aatttatttc ctcagcgatg
    14281 cccttttctg tatgtatttt gatctccatt ctcttgacga catcctacaa ccatatccaa
    14341 ttgatttttt atgagatggt taatcatttc tggagcagaa cttgcatcat aagtgtcatc
    14401 tccatcaacc attagataaa tatccgcatc tacatctgag aacatacgtc ttacgacatt
    14461 ccccttgcct ttcagaggaa cagagtgaac tgttgctcca gcatcctctg ccacttgtgc
    14521 tgtactgtca gtcgaattat tatcaaatac atatatagaa gcagttggaa tattcgtttt
    14581 gaaatcattt atgacttttg ctattgcctt ctgctcatta taacaaggaa taattacagc
    14641 gatattaaaa gagtgataca taagcccctc atataaacta tttagctaat gtgcaagtaa
    14701 ttgtgttttt aacaaatcgt ttactaacga aaaatgaaat attttgatgc gcaatatcct
    14761 aaaaccaaac taaaaagaga aaaggtgaac aatgtaaata ttggagataa agctaagaga
    14821 tcaaataaaa cgccgaataa ataactcatc gcccccataa aaaatataaa tataaaatat
    14881 ctagccttgg ttgcagatac tttaaatgag catcttgcat ttgcaaaaaa agaaaaggtt
    14941 gctgcacata taaaaccaac aatgttactt cggccttgac caaaagctaa attattataa
    15001 cacgcataaa atacccccca atgtaaagca gtattgacaa ttccaattaa aaaataacta
    15061 aaaaaagaac tggtcaataa cttccctatt tttaacatcc tttattttgc tccagaagtg
    15121 aggttattac taatttggat attttctata gaaaataccc ataaaactac aacgcctata
    15181 ataaaaattc ccatgggtgt aaaaagaaat ggattagtta tagaaacggt aatactgaaa
    15241 aaaagtatta acgacatcat taatgatgtt tttgtactat ttaaaaaata gcctttcaaa
    15301 aagataagca acataggaac taatgatata aatacaccaa taacgcctgt tttctgaagt
    15361 atctcaagag gtactatttc aatccttaat cgatctaata taaatgaacc aaatcctctt
    15421 ccaaacaaga acgtcgctaa atcaacattt ttataataaa aatataaatc attaaatcta
    15481 acagagtcag aatcttcact gcgtgatccg accatgtaaa gcataattat aaatagaacg
    15541 ctgcccaata ttattataat agtatttaaa tatagcttat gtaatttaaa actggctaat
    15601 aacagtgata atgttgtaaa aagtaataca cctcttgatt ctgtcaataa tatcgcaagc
    15661 actattatga gacattttaa atattttttg tcgacaaaac tgataatcgc accgacacca
    15721 aaaaagtaga aggatttgga aaaaaaagcc ccatcgggac gaaaaaagaa ctcgctagtc
    15781 aatgataagt gttcataaat taaattaaaa tttaacaaac cgaaattcaa caaaattatg
    15841 tatgaaatat ataacagtga tccatataat gcaaatgcat taactatttt aataaaaatc
    15901 tcacatgaaa atacttttaa agtatatctt agtgtaaata gatatataag tgacagcata
    15961 aaaaggtatg gttgcacatc aaacagtata tcatttattt tattaccagc taataagccg
    16021 acaaagaagc cataaaaagg aaataaaagc aaccatataa aaagactgac acatttttgg
    16081 gtgattgaaa aattgaatgt aactaaatta aaaattaaaa gtataaaact aaatacaaat
    16141 aataaatatc gcaaagggaa tattcccaat ggctccagta atcttccacc accaccaata
    16201 atcagttcaa tacataaaaa tgttataaaa attttattta tattattcat attcgtaagg
    16261 tgatgttttt taaaatatgt ttaaaatcaa cattaataaa atataaattt tatcagcctt
    16321 tttccgcctt aacttccaaa aacttaaagc caagatcaaa ttgcctatat aacctttacc
    16381 caattgatta ttcacaaagc tatcatcagc taaggcatta gctattttag ttacttcttt
    16441 tcgataccac ttgtttctca ccaaaccaag ccgtttgtat tttgctttaa aactaatgtt
    16501 tgctcccact tggttgtttt catgctgtct gtacagcatt gttggttttc tatcaatata
    16561 ccacgaataa tttctcgtcc gcgcaaatga atataaaaac caatcatgca gacagatatc
    16621 ttgtgcagca tttttattat ttatgatgaa cttcttaaat tcaataagcg tttctttttt
    16681 tataacatat gtacaccccg gacctgctgc ttcaaaaaaa taatcgaatt gagtttgcga
    16741 ctgagcctta tcaactaaat tttttcgccc tgatggataa tatgcaatta cattgctgga
    16801 ataacaattc gcattattaa ataccaagca gtcaattgca ttttttattt tatctttata
    16861 ccaaatatcg tcctgatcac taaaacagat ataatcatag ttttcaagat cgacatcttt
    16921 aattaatcta taaaaatttt tacctgcccc cccaaactta ttgacgcttg gaagtatatt
    16981 tataattgag ggatatgata attgaaattc ttcacaaata tttaatgtat tatcagttga
    17041 taaatcatct gatataaaaa ttgagatgtc tacatctttt tgttcaataa tactttttaa
    17101 ttgttcctca atccagcatt cgccattata cgtagccata agtacagcta ctcttttttg
    17161 atcagaacta tgcattataa cgaccgcccc cagtaattcc tcttattcca tccagcgtag
    17221 ccaaaatatg ttttttcata tattcaattt tatttttttg agttgcgata attaaagtcg
    17281 tatgaattaa agaaaaaaca acttccctaa tagagagtaa aagaggcaca tgattttttc
    17341 taagcaaaag aaatgcattt cttactcgat aataacgacg tactggcgag tgaattggta
    17401 tgttaatccc acagattttt actgacttat ccccaatagc atgaaccatt tctatatcag
    17461 gaatgatatg aactaatatg ccatagttta agcatctaag acaccattct gtatcgacat
    17521 aatcaataaa aagttcctca tccatcattc caacaatctc aaaaacttct tttgaaacca
    17581 tggttccgga tgagattgtt actgaacttt taaacggtgt ctgtccttca gtgacatgaa
    17641 ttttctctcg aagaccattt tttttgatat tacaaatagg ataataatgt gacttatctc
    17701 tatcaataaa aactgggcca gctatttttg cattttcatt atccatatag caggaaagtt
    17761 ttttgataaa ctcattgcga atacggctat cttgatcaaa aaaaattatc ttctctgccc
    17821 ctttttcaag agaatatcga atcccaatat tttgtgcttt tgctatacct tcattcctac
    17881 caagacaaat taaatgaact ttttcaatgt ctgctaattt atctttcaag gtgaaattag
    17941 tcctattatc gactactaca acgtaacaat tttgctcctt tagttcagtg atcagtgcat
    18001 tcagattttt aagttcggga ttatatgtca caatgacagc gtaaatatta ctattcatta
    18061 tccaagtgac tcagtaattg gttaatttta gttttcatat acagaacaca ttctagtata
    18121 aagccattgt aataacagag catctaaaat tacacgtatg ctccaggata ttgcggcacc
    18181 cagtaaccca tatttttcga ccataaaata caaaagggct aaataaggaa ccaattcagc
    18241 acaatgaatc aaagcagtaa ttttagattt tcctaatgcc tggattgcag agaatggtat
    18301 cattgccagc gcattgaaga aaaaaccgat caataacaca tttaatatat taaaggaatt
    18361 aatttgataa ttctcaccaa accatatttt caatacaagc cctgaatata acaagcctat
    18421 gattatcacc ggcaaacaga ttagaaacat taaaagcaat gatttattta cattacgttt
    18481 aaattcttta aagtctttga tgttacttaa ccttggaaac actgccctcc cgattgctgc
    18541 aggtattatt cctaatttta gaataacctc cgctggtgca gaataaaatg caactttatc
    18601 tgcccccata atatttgaca ctataaatcg atcaaaatac accatgaccg gacttatgat
    18661 attacttact gtcatccaac cgccaaagaa aaagagtcgt ttaaaggttt tataacaaaa
    18721 atgaaccccc gaaattttaa tatcattcct gacataatag gcagagacta aaatcgaaat
    18781 aactcttgca aatattaaac cagcaaccgc tgccgacaat gtagcactat aaaaaacaaa
    18841 tatggccgga attcccgcga tgcaagagct tgatatagat ttttgaatat ttacaatgcc
    18901 aaatttttca tccccctcaa gaatggctga ccataattga tttagaataa ataatggaat
    18961 gcaaatagcc aacagtttaa atgctagttg tatatcacta tgttcaacac cagaaatttt
    19021 tagataatta acaattccat cagagaaaat caataataaa aaagcaccaa agcatgaaaa
    19081 tagcaccaaa aaagatgtac ttgttgaaat taccttggtt ctttcatgat gattatcacg
    19141 atgaattgca atttctctaa taacagagcg agtcaggcct acatcaaaaa ttccagcata
    19201 cccaacaagt gcaattgcaa ttgtataaac accgaaacct tcaggcccca aacctcgagc
    19261 gagaaatcct agcgagggga tagcaattaa agttggtatt gcatagccaa aaaggttcca
    19321 gacactattt tttattatgc tcattattac atttctttat catataaaat aatcagcatc
    19381 tacaaatttt tgaccattaa tatctttatc agaaagcaat ggattttgaa ccggccattc
    19441 tatatttata cttttgtcgc tccataaaat cccacgctct tgcatttttg aatagaaatt
    19501 atttgttttg tagacaaatt ctgcttcatc actaagaacg caaaacccat gagcgaatcc
    19561 ttccggtatc cataattgac gcttattctc cgccgacaaa tttacaccaa cccacttgcc
    19621 aaatgttggt gatgatttcc gaatatcaac agctacgtca aaaacttcac cgacaacaca
    19681 gcgtaccagt tttccttgag cataaggttc cagctgatag tgcagtccgc gtaacacacc
    19741 cttagttgat ttagaatggt tatcctgaac aaactcaacc tttcgtccta cagcctcttc
    19801 gaaaactttc tgattgaagc tttccataaa aaagccccgc tcatcaccaa atacttttgg
    19861 ttcaaaaatt agcacatcag gaatttcagt tttaattacg ttcattttat taataacctt
    19921 taatcatttt cagcagatac tgaccataag catttttctt cagcggctcg gctaatacct
    19981 tcacttgctc agcatcaata aagcctttac ggtgagcaat ttcttccggg caggaaactt
    20041 tcagcccctg acgctcttca attgttgcaa tgaagttgct tgcttcaata agactttgat
    20101 gtgtccccgt gtccagccat gcataaccac gccccatcat agcgacagac aaacgtcctt
    20161 ggtccatata aatacggtta atatcggtaa tttccagttc gccacgtgca gatggtttaa
    20221 ggtttttcgc catttctaca acgtcattgt catagaaata aagcccagta accgcatagt
    20281 tactttttgg ttcctgcggt ttttcttcca gactaattgc cgtaccgtta tcatcaaatt
    20341 ccaccacacc gtagcgctca ggatcattta cgtgataggc aaaaaccgtt gcgccactct
    20401 ctctgttaac agcggtatcc attaatttcg gcaggtcgtg accatagaag atattatcac
    20461 caagtaccag cgcacaatca tcaccaccta taaattcttc accaatgata aatgcttgcg
    20521 ccagaccatc cggactcggt tgtactttat attgcagatt aagcccccac tggcttccat
    20581 caccaagcaa ttgttgaaaa cgtggtgtat cctgcggcgt actgataatc agaatatctc
    20641 gaatacccgc caacataagc gttgaaagcg gataataaat catcggttta tcataaatcg
    20701 gtagcagttg tttgctcaca gccattgtca caggataaag acgagtgccg gaaccaccag
    20761 ccagaataat acctttacgc gttttcattt caccattcct tttaattcat ctcgctctag
    20821 catcatgagc gagatgcaat aatttgttaa attgccgtgg tcgtaaataa ttcgttgagc
    20881 atacgtttca cgcccacctg ccagtcaggc aagacaagcg caaagttctg ctgaaatttt
    20941 tcggtattga ggcgagaatt atggggacga cgtgctggtg tgggataggc cgttgtcggc
    21001 acggcattaa gtttgttaag agcaaggttg atccccgctc tgcgcgcttc ttcaaatacc
    21061 agagcggcat aatcgtgcca ggttgttgta ccaccagcaa ccagatggta caaaccagca
    21121 acttctggtt tatttgcggc cacgcgaatg gcatgagcgg tgcaatcagc cagtaattca
    21181 gcccccgttg gtgcgccgaa ttgatcgttt attacagcca actcttcgcg ttcttttgcc
    21241 aggcgcaaca tcgttttggc aaagttattt cctttaccag cgtataccca gctggtacgg
    21301 aaaataagat gctttgcgca atgttcttgt aacgcttttt ccccagctag cttggtttca
    21361 ccgtagacat ttagcggtgc tgttgcatcc gtttccagcc atggcgtgtc accgttgcct
    21421 gggaaaacat aatcagtgga gtagtgtata acccaggctc cgacttcatt agctgctttt
    21481 gcaatcgctt cgacgcatgt cgcgttaagt aattgcgcga aattcggttc tgattccgct
    21541 ttgtctactg cagtgtgagc agcagcatta acaataacat cggggcgaat ttttttgacg
    21601 gtttcagcca caccttcggg gttgctgaaa tctccacaat aatcagtgga gtgaacatcg
    21661 agcgcaatca agttacccag aggagccaga gcacgctgca gttcccaacc cacctgccct
    21721 gttttgccga aaagcaggat attcattact gacggccctc atagttttgt tcaatccacg
    21781 actgataagt accgcttttc acattctcaa cccaatttgt attagccaga taccattcca
    21841 ccgttttacg aatcccgctc tcaaacgttt cttgcggttt ccagcccaat tcgcggctaa
    21901 ttttatcggc atcaatggcg taacggcggt catgccccgg gcgatcggca acataagtaa
    21961 tttgctcacg ataagatttc tctttcggta caatctcatc caacaaatca caaatagtga
    22021 gcactacgtc gatgtttttc ttttcgttat gtccaccaat gttataagtt tcacccgctt
    22081 taccttcggt tacgacggta tataacgcac gagcatgatc ctctacatac aaccagtcgc
    22141 gaatttgatc ccctttacca taaataggta atgccttacc ttccagagca ttcagaataa
    22201 ccaatggaat aagtttttcc gggaagtgat aaggaccata attgttcgag caattagtca
    22261 caatggttgg taaaccataa gtccgcttcc aggcacgtac caaatgatcg ctggaagctt
    22321 tagacgcaga atacgggcta cttggcgcgt aagctgtcgt ttccgtaaat agcggtaacg
    22381 cttcgttatt atttgcttca tccggatggg gtaagtcacc ataaacttca tcagtagaaa
    22441 tatgatgaaa gcggaaactt tttttctttt catcattcag agcagaccag taattgcgcg
    22501 ccgcttctaa aagaacataa gtccccacaa tattggtttc aataaatgcc gcagggcccg
    22561 ttattgaacg gtcaacgtgg ctctctgccg ccaggtgcat caccgcgtct ggctggtgct
    22621 gtgcgaaaat acgcgacatg gctacagcat cgcagatatc tgcatgctca aaagcataac
    22681 gttcagaatc agaaacatca gcgagtgatt ccaggttccc ggcgtatgtt aatttatcga
    22741 cattaacaac actatcttgc gtattattta ttatgtgacg aacaacagca gaaccaataa
    22801 accctgcgcc accagtaaca agtatcttca cttttttatt ccatatagct atagagcatg
    22861 ctgtgaaata gactgctcca gatttgatta atagaggtat taatacacgc taccgcccct
    22921 ggcttaacag ctaccagtgc actgcatgca tctcaacgat gtggcgagcg ttagccactc
    22981 gcgccaaatc cgaaaaattc aaacgttaat tgtcttacca aacagccgta gaaacaagga
    23041 aaatcctgga aaaatttgaa taatatgagc tatgtaactt cttgttttta taggctttat
    23101 aaaaacggca acggcaggaa aattcaatga gcaaaactta tgccacgaat cttcactgcc
    23161 gttataattt tcttatcaac cgttacatcc ggtcagattt tcattattcg cttaacagct
    23221 tctcaatacc tttacggaac ttcgcccctt ctttcaggtt gcgtaggcca tacttcacaa
    23281 acgcctgcat atagcccatt tttttgccgc agtcgtaact gtcgccggtc atcagcattg
    23341 catcaacgga ttgttttttc gccagctcgg caatagcatc agtcagctga atacgtcccc
    23401 atgcaccagg ctgagtacgt tccagttccg gccaaatatc ggcagaaagc acatagcgac
    23461 ctacggccat gatgtctgag tccagcgtct gcggctgatc cggtttttcg ataaattcaa
    23521 caatgcggct gactttaccc tcacggtcca gcggctcttt agtctggatg acggagtatt
    23581 cagagaggtc acccggcata cgttttgcca gcacctggct gcggcccgtt tcgttgaaac
    23641 gtgcaatcat ggcagcaagg ttgtaacgta gcgggtcggc gctggcatcg tcgatcacaa
    23701 cgtctggcag taccacgaca aatgggttgt caccaatggc aggtcgcgca cacaaaatgg
    23761 agtggcctaa acctaaaggt tcgccctgac gcacgttcat aatgatcacg cccggcggac
    23821 agatggactg tacttccgcc agcagttgac gcttcacgcg ctgctcaagg agtgattcta
    23881 actcataaga ggtgtcgaag tggttttcga ccgcgttctt ggacgcgtga gttaccagga
    23941 ggatttcttt gatccctgca gccacaatct cgtcaacaat gtactgaatc attggcttgt
    24001 cgacgattgg tagcatctct ttgggtatcg ccttagtggc aggcaacata tgcatcccga
    24061 gacccgctac aggaataact gcttttaaat tcgtcattat ttcatccacc tgtaaaatgg
    24121 ttgctgaatt atagcttgtt cgattttttt cgccagcatc aattaccctg aattgattac
    24181 tgaattactt gtgatgttac gccgcttcgt tgtggattgc agtagcattg ttcctaagta
    24241 tggctccatt tttccaggaa tggtcgcaaa tctactccct ccgttccggc aatctaaagt
    24301 taatcttttc cacattaaca atatggtgat taatcctgtc gatatcgacg gagctttgcc
    24361 ctctttcatt caccgcatga acatttgcaa gagacagcag tgtttctttt ttcgccataa
    24421 agacgccgcg aacgtctttg cgcatgtcga agttcatgct caatgctggt ccaactgagg
    24481 attcctgcat cacattgata tttcgcataa aaagatgttg cggcttgttg tgtaactcca
    24541 gcgaggcacg cttcatctca atgttagtta gcgacacaaa ggagacggca ttcccggcgg
    24601 agatttggat gccgcgcaat ttataagcaa gatgggtatt atccagttga atattattca
    24661 ctcggaaatt ttgcggtatc gagagatatt tgcctttaat taccccatag ccgattaaca
    24721 tcccggcgct attaatcatt tcaatattat caatcacgaa attgtcacaa ccgtaaatag
    24781 cgactgtcgc gttatcaatg cccgctttct tactgaaatc cggcgtgata ttgcgggcat
    24841 tgatattacg aataacaaaa tgtttaccat tttcaacatg gatcaactgc cgacaatccg
    24901 atcccgtgat attcgccacg acaaagtttt tcactgcctg gtcttccggg tagttgttat
    24961 cataagtgct tcccgcaagg cctatgccga tgccccagtt gattttgccg ttggtacagt
    25021 tgatacgctc gatgacatgg tcagatatca aaatatcact gtcgttaatt gccacgttcc
    25081 attcaatggc gtcgccctgt aagtcgctga acttacagtt ggtaatgttg gcaccgataa
    25141 tctggttatg aaatccctgg cgtaagatgg cgtaattagc gtggctgaca gtgaggttat
    25201 cgatggtcag gttgcgcatg acccgtttgt ttttgccgcc aatataaatc tgcattaccg
    25261 ggccaaagcc gctcatcgcc agccctttga tggtgcagtc agaaccacgc acatccaggg
    25321 taatgttatg cataccgccg ccctctcccc ctgtcacctg gctgccgtcc tgtaagacaa
    25381 atcgccctct gccgttgccg cgcaggcttc caaggatgtg taacgttttg ccgggaggga
    25441 tgaagatgcc ggtgttgata ttgtcacaaa cgaatccggc aggcacgacg accgtttgcc
    25501 cttcgctgaa ggcttgttta aatgaggcga tccagtcatg tgggttgtag tcgttaatgt
    25561 taacgctttg tcgggcggga agtgcgcgag cgaaaggggt atggaggaag gcaagcgccg
    25621 agcttgccgt caggaaggtg cgtcgggaga gtgttttaaa tggcatacgt tctcctctat
    25681 aaagcctgca gcaagctggc gagttctcga ttaatcacct gctggttaaa atcgtgttcg
    25741 actttttcgc acgcacgttt gacgaccgga gccagttcgt cggtatccag ttggctaaac
    25801 gccgccagac gttgtgccag tgcgcgagca tcgttctcag gcagcagcca gccggattta
    25861 tcggcctcca ccagttcagg aattccgcta tgcagagtag aaaccaccgg aataccgacc
    25921 gccatcgctt ccattagcgc caccggaatg ccttccatat cgccatccgc gcccgttacc
    25981 gatggcaaca ggaagacatc cgcgtcgtcg agcatctcct tcacttcatg gctcggttta
    26041 aagcccggca tctctaccac atcttccagt tgatattgtt cgatgatggt gcgcaggcgt
    26101 cgttcccaca ggccaatgcc gaggatgcga tagcgaaatg ccacgccctg ctctttcaac
    26161 tgacggcagg cttcaatcgc cacatgcagg ccttttttct cggttaagcg tgcgacagat
    26221 atgatttcca gcggcgttgc gggcgctttc accggacgcg ggctaaagcg cgtcatgtcc
    26281 acgcccatac gcgatacggc gattttttct ctggggcagc ccattttttg cagccttccg
    26341 gcccacagat cgcttatcgg taacatcagg tcgccacggc gaaacagttg ttgatattcg
    26401 ggagtgtagt ggttgagcac ttcccgactg gagatatcaa taccgtggaa gatggtggca
    26461 attttgccgc gaatgatacc cagttcgcgt agttttgctg cggttacccc cgccgggcca
    26521 aaatgagcga tgaacacatc ggcgcgaaac ggtgttgcga cctgaccaca aatggcagac
    26581 aaaatcaggt tccgcgactc ggcaccatag cgtttgaggt tgagcgcatg ccaggtattt
    26641 ttacgatgaa tgccacgtaa ggtctggctg gcgcggtggc gcagtttcgc tactttgccc
    26701 gcaggttcgt cctgtaacca gcgggttctg gcgacaaggt tgtatttcgt ccatgccgcg
    26761 tgggtgttct ctgtatcgcc tttttgcaaa gcgacaatct ccacttcaaa gcccatatca
    26821 ataaacgcgg taatttgatt gaggacgaag gtttccgacg acagcgggaa tttcaataag
    26881 aagaagccga ccttcatttc acctccccga tgcgctcaag cacagactgc accatctgca
    26941 ttcccgtctg acgttcgcga ctgacggctt cgttaaatcg agcgttcagc accggaagct
    27001 ggcctaaggt atccgcaacc atcgcttgca ggctgccgtc cagtaaatga cgaatatcaa
    27061 tcgccatctc tggcagtccc agctgctgca taatcccggc ggatttatgt tcatagttga
    27121 tggcaatcgc cggagtagca aagttcattg agataatggc ggagtgcagg cgcgtaccga
    27181 cggtgagttc acaggctccg agaattttgc ccatttccag atcgttgagt tcatccatca
    27241 ctacgtggta acgggcagga tcgctgatgt gctggcgcag attgagcgcc accatgcggt
    27301 cgtctttgtt atagctgtcg atacccgtac aggtggagag cgcaatcacc tgatagcctt
    27361 catcgagaat gcgattgacc accccggcaa aggctttttc atacgcttgt tgagtggtgc
    27421 cgagacgttt gtcgaacggt gccagttcgc gcagggtaat tgccactgtt ttctgttgtg
    27481 cggcaacgtc cagccagtgt tgaacggcat agctggcggt gaagtcttcg gtgtggtgat
    27541 cgaccagcca cgcggtatcg acgccatgtt ccacttttgc ggtggtgata tttctgcgtt
    27601 tcatcaaatc aaggctgacc gattcgcgca ggatcagcgc gtcgcagtga ccaaagacat
    27661 agttcgccag ttggttaaat tgctcatcct ggaaggggcc gacgctgtga ccaatcataa
    27721 acagcggctt tttcgccata aatgtgcaaa gcgcatgttc aaactgcggc acgccgtaga
    27781 gatcgacaaa aaacgatccg ccgacctgaa taatggcgtc gtaacctgac agcaggcgca
    27841 cgaagtcggt gaatccctgg gcgatggcga tattgcgcag cttgccagtg tcggtgacgc
    27901 gtgagagcaa tacctgatgc tggtagcggc gacggaggac ttttttaatg cgcccgacaa
    27961 cgcccgccgc gctattgtgt tgtgtcatct gcaggaacag cggatcgccc attaccgggc
    28021 ggttgagcag ccaggaagaa ctgaccggat agcggctcat cacgtccact tcggtatgtg
    28081 gattgaggat gttgatggca tcaagtaagc cgcgcaagat ggcgctgtcg ccacgattgc
    28141 cgcaagtgtg gttgcccaga ataagtaatt tcattttttc ctcataaatt tgatgccagg
    28201 tgaggctgtg tttgttgtgt tcgttgccgt tttttgcctg atgcgacgct ggcgcgtctt
    28261 atcaggccta caggtcccga gcacagaacc gtaggacgga taaggcgttt tacgccgcat
    28321 ccggcaaccg ttgtcggaac cgaaaacagc aattcacccc gcccgtaaaa gcattttcat
    28381 tttttcgctg cgacaaaact gacgcttcac ttccaccacc agcggatggc gtgacagcac
    28441 aatcatcacc acaaacgcca gcacgcccgc ggctatttgc accgccagca gcatccccag
    28501 cgccagttgc cctttcagca caatgcccag cgcataactg accaccagcg tcggcaacga
    28561 gagataaaac ggcagccaca aactcaggat gtactggcga taactggaac caagaaccgg
    28621 tttaatcatc acgaagtaac tcagaatggt gttgataatc tgcaccagca ggaaaccgag
    28681 cgtgacgccg attgcgcccg ccatctgccc accaataaca atcgccggaa taaacagaaa
    28741 tgttttgaat acgttgaatt taaagctgat atcgacccgc gctttcgcca tcagcagcga
    28801 accaatcggg ttacccacgg agcgcagcag acccacaata cacagcaatt gcagcaccgg
    28861 aataatgctg ttccactttt cgccgaatac cagcggtacg aagttattcg acaccaccat
    28921 cagccccagc agcgccggaa agttgataat ccccactatc gacagcagct tgtagaagtt
    28981 aacacgcagc ttttcggtgt cgtcctgaat tttggcaaac gccggaaaca acacgcgggt
    29041 gatgattggg ttcagcttca tcggcggtac gacggccacg ttgtacgcca ggttgtatcc
    29101 ccctgccacg cccgcgccaa gaatacgcgc cagcaccagc gttgaaaggt tggtgttgag
    29161 atagttgatg atgctgtccg ccgtcagcca ggcaccaaaa cgtaagttcg gtgccacaga
    29221 cgccagcgag aagtgcagac cggggcaata aattttgcgt ccaaagtagc caaacagcag
    29281 tgttctcacc gcactattga ccagataacc gaggatcgcg gtcatcgcca gcggccagaa
    29341 atgggcgcta actaccgtaa aagtgaagcc cgccagcacc gcgctggttt cgatcatgcc
    29401 gattttgttg aactccagct ccttctgcat caacgcgcgg aactgttgcc cgtgggggat
    29461 caccacaaac gccagcgaca atgttttaat caacggtgcc aggtccgggt tattcagcac
    29521 gtcgccgatg acatcactca acaaaaacac cgccacacat accacaagcc ccagcccgac
    29581 gttcagccag tacagcgtgg tgagttcaag gtgactgatt tcttttcgct gaataatcga
    29641 gttagcgata ccgaagtcag aaagcgtatc tgccagcgcg ataatcacca gcgacacagt
    29701 cagcaggccg aactggtggt tgtcgataat ccgcgccagc acggtcatct gcaccagccc
    29761 gaggccgatg atgatcaccg tggcaatagc cgaccacttc gcgccgctga tggttttttc
    29821 acgtaagctc atatcaatat gccgctttgt taacgaagcc cttgaatacc gtcaggaaaa
    29881 cgattttgat atcgaaccag acgctccatt cgcggatgta ctcaaggtcg aactcgacgc
    29941 gtttttccat tttctccagc gtgtcggttt cgccgcgcca gccattaatc tgcgcccagc
    30001 cggtaatgcc cggtttcact ttatggcgca gcatgtagcc ttcaatgagc tggcgatact
    30061 gttcgttatg cgctactgcg tgcggacgtg gaccgacaat cgacatcccc ccggtcagca
    30121 cattgataaa ctgcggcaat tcgtccagcg aggtgcggcg cagaaagttc cccactttag
    30181 tgacgcgcgg atcgttctgc gtcgcctggg tcaccacttt gtcgttctcc atcactttca
    30241 tggaacggaa cttccacact ttgatcggct tgccatccat accatagcga gtctggcgga
    30301 aaataaccgg ccctggtgaa ctgagtttca ccgccagcgc aatacagcac agcaccgggg
    30361 agatcagcag cagaataagc gtcgccagca caatgtcttc cgcacgtttt agcaggcggt
    30421 taacccccga aagcggcgtg tcatacagcg gcaccaccgg aacgccgttc atctcttcga
    30481 gacgtgaatg gagaatgttg aaggtaaaga cgtcggggat cagcagcacc gaacaggtgg
    30541 tatccgccag ttgatggacc agttttttca ctcgtgcgcc gtcgcacatt tgcatcgcga
    30601 tatagacgtt atgaatcttg cccgcttttg catcctcgac cagctgttgc aggttacccg
    30661 cccagtcgtt agaaacgccg cccggtttcg ggtcgtggta aacgcccacc acttcaaacc
    30721 ctaaccacgg ctgattacgg aaactctcca tcagcatttg tccggcggct aaatcccccg
    30781 ctacggcgac catccgcttg ttgtagccat gattacgcag ccagcccgcc ccaatgcgaa
    30841 tacatgaacg gcaaaccacc agtccgatgc tggtcagccc ataccacgcc agccagattt
    30901 tcagttgcgt gtcgaaatca ttgttgaacg ccaccagtcc ggcgctgaaa atcacgctta
    30961 aggtccagtt ttgtagcaac agggcaaatt ctgtcgctgc ccgaacaccg cgccatgagc
    31021 gataaaaatc ggtgatgccg cccagcatct ggaacaccac cagcgtaatc agcgccacca
    31081 acaggtgcat gtagaggaat gacagtccgc tgacttcgca aaccagccat agcccggcaa
    31141 acatgatggt gatatctgaa aagcgttgca ccatagagat taacgatgca ttggttttcg
    31201 ctcgctcgcg cttttttaga tttgtcatcg ttgttcctgt tattagcccc ttacccgcag
    31261 tggggtaagg gaagatccga cattactcgt tcagcaacgt cagcagagtt cgcgttcgcg
    31321 cttccatcag cggcacatca ccgcgcgatt ccacattcag gcgcaccacc ggttcggtat
    31381 tggaggtgcg caggttaaag cgccagtcgg caaaggtcat gctgatgcca tcgctgcgat
    31441 ccaccgccag cgcctcacgg ctaaaatgct gttccacgcg gttaatcgcc tcaacgggtt
    31501 gcgccagttt gctgttgatc tcaccgcttg ccggaaacgc cgccatccgg tcgcgcacca
    31561 gttcgcccag cgtttttcct ttcaggcaca ccagttcggc gaccagcagc cacgggatca
    31621 tgccgctgtc gcagtaagcg aaatcacgga agtaatggtg ggcgctcatt tcgccaccgt
    31681 agatggcgtc ttccttgcgc atccgttctt taataaaggc gtgtccggtt ttcgacatta
    31741 ccggcgtgcc gcctgcagca gtcaccacat caatagtgtt ccaagagaga cgcggatcat
    31801 ggatgatctt cgcgccggga tttttttcga ggaacgcttc tgccagcagg ccgacaatgt
    31861 agtagccctc gataaactgc cctttttcgt caaacagaaa acagcggtca aaatcgccat
    31921 caaaggcaat gcccatatcc gcgccgtgtt tgatgaccgc attgcgggtg tcgtcgcgac
    31981 attccggcag cagcgggtta ggaataccgt tggggaaatt gccgtccggt gtgttgtgta
    32041 ctttgattaa ttccacgggc gcgccgaggg ctttaaagcg ggcttcaatg gcgtccacca
    32101 ccggacccgc tgcgccattc ccggagttga tcaccagctt gagcggcgtg agatttttga
    32161 cattgatata accgaacagg tgatcaacgt aagcgtcacg caggttgatt tgctgatagc
    32221 gaccgcgttt ggtttcatcg acgggaggaa agtcgttggc ttcagccaga cgctggacgt
    32281 cgcgcagtcc ggtatcaccg ctgatcgtgc gagccccctc gcgcaccagc ttcatgccgt
    32341 tgtaatccat cggattatgg ctggcagtaa cttcaatgcc gccatccacg ccgagatgga
    32401 acgtggcgaa atagatctct tcggtgccgg acataccgat atccagcaca tcgacgcccg
    32461 catcctgtaa accttttgcc agcgccagtt ttaaggtttc gctggtgaga cggacatcac
    32521 cgcctaacac aatggttttc ggtttgagaa attcgccata ggcgcgacca atgcgccagg
    32581 cgatatcttc attcagttct tcgcctaatt tcccgcgaat atcataggct ttaaagcagg
    32641 ttaatttttt catgtcgtta ccctttttca ggcaatagtt ggccctgacc gaaacggcca
    32701 attttatttt tgtcgttatt tacaccgcgg tttcgcattc attgcctgat gcgacgctgc
    32761 cgcgtcttat caggcctaca aaaccgaacc gtaggtcgga taaggcgttc acgccgcatc
    32821 cgacaaacag cgcctgatgc gacgtttaca cccgtccgta gcgatccgcg aagcgcacca
    32881 catcatcctc ttcgagataa gagccggagc gcacttcaat taaatcgagc ggaattttcc
    32941 ccgggttttc caggcagtgc gtcgccccca gcggaatata aatggactcg ttttcaccaa
    33001 gcagtttgat atcaccatca atggtgactt ttgccgttcc cgcgaccact acccagtgtt
    33061 ccgcgcggtg atggtgcatc tgtaccgaca aaccctcgcc cggtttcacg gtgatgcgtt
    33121 tcacctggta gcggtcgccc gcgtcgatag agtcatattt gccccacgga cgatacactt
    33181 cgcgatgcac ccgatgctca tggcgaccat cggctttgat ctgctcgacc acttttttca
    33241 catcctgtac cgcgttacgg tcggcaatca gcaccgcatc tttggtctgc accactacca
    33301 gatctttcac cccgacggtg gtgaccaggc ctgattcggc atacacatag ctgttttcag
    33361 ttttgtgatt aatcacatcg ccgtggcaaa cgttgccctc ggcggtgtgg gcgctgatct
    33421 cccataatga agaccaggaa ccaacatcgc tccagcccgc atccatcggc accacaacag
    33481 catctgccgt aggttccatg accgcgtaat ccaccgactc ttccgggcag gcgagaaacg
    33541 cttcttcatc cacacgaata aaatcgagat ccggatcgac ggcgctcatc gctttttcgc
    33601 aggcatcgag gatatccggg cgatattttt tcagttcttc gagatagcgt ccggcgcgga
    33661 acaggaacat gccgctgttc cagtaatatt cgccgcttgc tacataggcc tgggcggttt
    33721 ccagattcgg tttttcgaca aactgcgcca cttcaaaggc caccgtatcc tgctcaccca
    33781 cagacacttc accgcgacga atatagccat aaccggtttc tggtagatcc ggcacaatgc
    33841 caaaggtcac cagcttgccc gcttcggcat aaggcatggc attacgcacg gcggcacgga
    33901 acgcgtcttc atcggcaatc acatgatccg ccgccaatac cagcattaac gggtcgctct
    33961 ccgggctatg acgttttgcc gccagcgccg ccagtgcaat ggcaggtgca gtgttacgcc
    34021 ctgccggttc gagaataatg ttctcggtga gtttgttcag ttgacgcagc tgttccgcga
    34081 caataaagcg gtgctgctca ttgcaaatca ccaccgggct ttcgcactcc acgccgttca
    34141 ggcggcagat ggtggtttgc agcatggtga gatcgccttt caggcataaa aactgcttgg
    34201 ggtaaagtac gcgggaaagc ggccataaac ggctaccgga gccacctgcc atcacaactg
    34261 gatagagttt cgactgcgcc ataattatcc ccgaatatca tttataaatt gacgtaacac
    34321 gttctcttta tcgagcgtgc gttcggcata ttcacgtgcc accgtgttat gtttgggcag
    34381 caggagcgcc tgacgaatcc ccgccaccag cgcctcgacc gattccggtt ctacgcaaac
    34441 cgcaataccc ggaaaggttt cgcaaagctg ccccagttct gtgtgggcct ctgcggtaat
    34501 caccgcgtta ccgcctaccg ccagaatatt ggtcagtttc gacggcaata cggcatctgc
    34561 cgcgccgcgt ttttgcacca ccagatggca atcgcccatc ttcagcagtg caggtaaagc
    34621 gtcatacgat tgcagcggga aaaattgcat gttgcgcagt ccacgctgct gcgccatttt
    34681 ttccagccgc gctttgccgc cgccctgccc gacaatggca aaaatcagcg gttcatcgcg
    34741 caggagatcg gcagcttcaa taacgttttc cagcccctgc ttttcaccaa tattgccgga
    34801 gtaaagaatg atttttttgt tatccggcag gccaagctgg ttacgaaggg catcaacatc
    34861 ggcgtctgca acatgctgaa aacgggcaat ttccgaccag ttggggaaga agatgacgtt
    34921 atccgccgcc acgccttttt cgatggcttt attcatcatc gaacgcgaaa tcgtggaaac
    34981 gttatcgacg ttatgcagtc cgctacgttc gaacgccgtt gccagctgtg ccactttgcc
    35041 gcctttgcct tttccggcaa ggcccagccc cagcatggcg tccacttcgt aatcctgaat
    35101 atgcagcacg gtacgcgcac cagagagctt cgccagcagg cgcattcccg gcgtgcaaaa
    35161 cagcgttggc actacgccaa taatgcgatc cggcttccag cgacgttgcg ccatcagtgg
    35221 aaagaaactg ctgacggcaa aactgccgag atgcaacaag cgtttcaggg tgctcggctg
    35281 ttttggcacg tacagcgggc agcgccacac cgtggcggcc ccttcttctc gtttgtagcg
    35341 ccaggcggaa tagttctcac ccacctgcca ttgcgggtag taaggcggtg cggtaatgac
    35401 ccgcacctca tgaccttgtg ccgccaacca ttccaccatc tcgccggtgt atttgccgat
    35461 gccggttaac tccggcgagt agttaatgcc gtagaccagt attttcataa tccgggtact
    35521 ccggcacgct tctcagcgag aaaataggct cggctgttag cgtgaacatc gttactggcg
    35581 agcagcgcgt ccggcgtcag ccagcggtaa tcgtcatgct gctcatccgg cagtaacagc
    35641 tcttcttccg ctactctgaa gcgaaaaccg agcaccacat agtgagtggt gaaatccgtg
    35701 ccggagaagt tatcgtcata aaaatgctgc cagacaccgt aaaactggcc tgctgttatc
    35761 ggcagacgca gccccagttc cgccatcgtc agccgctcaa atgcggcttc cagcgtttcg
    35821 tctttctgca cgcgccctcc cggcacaaac cagtaaccct gcgccgggcg gttggttctt
    35881 ttgccaagca gaaactcgcc gcgactgttc tcgacaataa agtcgagaga gacaagcggt
    35941 gtggagcgca ctacagtggc aaagtcttcc tgacgtaaaa acatcattcc ccccgaaagc
    36001 ggtcttgatt ctcaaggaac cactggtaag tgctggcaag ccccgcttcc agtgagattt
    36061 cgtgatacca gccaagctga tgcaggcgcg tcacatccag cagtttgcgc ggcgtaccat
    36121 ccggtttgct ggcatcaaaa accacccgac ctttgtaacc caccactttg gcgatggttt
    36181 gcgccagctc gcggatagtg cagtcaacgc ccgtgccgac gttaatgtgc gacagcatcg
    36241 gttgggtatt ctccagccag acttcatgcg ccagctccat gacatgaatg ctcgccgccg
    36301 ccatatcatc gacgtgcagg aattcacgca tcggtgtacc gctgccccat accaccacgt
    36361 ccggcgcatt ctgtgccgtc gcctcgtgga agcgacgcag caatgctggt atcacatggg
    36421 agttactcgg gtggaagttg tcatgcggcc cgtacaggtt ggtcggcatg acagtgcggt
    36481 aatcgcgtcc atactggcgg ttgtaagatt cgcacagttt gatcccggcg attttggcaa
    36541 tggcgtaagg ctcgttggtc ggctccagcg tgccctgcaa taactcgctt tctgccatcg
    36601 gctgttttgc cagtttcggg tagatgcagg acgatccgag aaacatcagt ttgttcacgt
    36661 cgttctgatg cgcggcgtga atgatgttgc tctcaatcat catgttctgg tagatgaaat
    36721 ccgccggata ggtgttgttg gcaacaatgc cgcccacttt cgccgccgcc agatagacct
    36781 ggtcaatgcg ttcgctggca aagaaatcat gcacggcgcg gctgtccagc aggttcagtt
    36841 catcgcgggt acgtaatacc agctccacat caccgcgctg ttcgagctgc cgcttgatgg
    36901 cagaaccgac catcccacaa tggccagcga taaaaatgcg ttgtttactc atgcttatga
    36961 ctccagcgcg atcgccacgt cgtagccgtg agatttcagc agagagtgtt ttttcgccgc
    37021 ttcgaggtca ttagccacca tttcagacac catctctctg agggtgattt ccggtttcca
    37081 gcccagtttt tcgtgcgctt tggtcgggtc gccgagcagc gtttcaactt ctgccggacg
    37141 gaagtaacgc ggatcaacgg cgataatcac atcacccggt ttaacgcccg gcgcgtcatg
    37201 cccggtgacg gaaaccacaa tgcccttctc ttcaacaccc gtgccttcaa agcgcagttt
    37261 gatgcccagc tgtgctgccg ccatttccac aaactgacgt acggagtact gaacgccggt
    37321 cgcgataacg aaatcttccg gctgttcctg ttgcagcatc atccactgca tttttacgta
    37381 gtctttggcg tggccccagt cacgcaggga atccatattg ccgaggtaca ggcacgactc
    37441 cagcccctgg gcgatgttgg cgattgcgcg ggtgattttg cgggtaacga aggtttcgcc
    37501 gcggcgcggg gattcatggt tgaagagaat tccgttacaa gcgtacatgc cgtaggattc
    37561 gcggtagtta acggtgatcc agtaggcgta cagtttggcg accgcatacg gagatcgcgg
    37621 gtagaacggc gtggtttctt tctgcggaat ttcctgcacc agaccataca gttcagaggt
    37681 ggaagcctga tagaaacgag ttttcttttc cagaccgagg aagcggatcg cctccagcag
    37741 gcgcagcgta cccatcgcgt cgacgtcagc ggtatattct ggtgactcaa aagagaccgc
    37801 aacgtggctc attgcgccta ggttgtacac ttcatccggc tgtacttcac gcaaaatgcg
    37861 cgtcaggtta gaggtatcac tcaggtcgcc ataatgcaga tggaatttcg ggttgcaggt
    37921 gtgcggatcc tgataaatgt gatccacgcg ctcggtgttg aatgacgatg cgcgacgctt
    37981 aataccatgc acctcgtaac ctttttccag cagaaactct gccaggtaag aaccgtcttg
    38041 tccggttaca ccggtgatga gagcgacttt tgacatgtct tattcctctg tatttttgaa
    38101 tttattcagt ttcaacgcgt tcgcgtatca ccactgcggg attcccccgg caaaccacat
    38161 ttgccggaag cgatttaaaa acactgcttc gtgcacccac gacggtgccg tcgccgattg
    38221 tgacgcctgg ggcaacaaag acatcggttg ccagccagca tttctcgcca atcacaatag
    38281 gcgtggcgtt aatggtgaaa tgttgacttg catggtcgtg gctaccggtg cataaataac
    38341 ttttttgcga tatcaccgaa tgt
//
LOCUS       DAJTRC010000029        38089 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.029, whole genome shotgun sequence.
ACCESSION   DAJTRC010000029 DAJTRC010000000
VERSION     DAJTRC010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 38089)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38089)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38089
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.029"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(33..1142)
                     /gene="anmK"
                     /locus_tag="OO357_001600"
     CDS             complement(33..1142)
                     /gene="anmK"
                     /locus_tag="OO357_001600"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HCR5886586.1"
                     /translation="MKSGRFIGVMSGTSLDGVDVVLATIDEHRVAQLASLSWPIPVSL
                     KQAVLDICQGQQLTLSQFGQLDTQLGRLFADAVNALLKEQNLQARDIVAIGCHGQTVW
                     HEPTGVAPHTLQIGDNNQIVARTGITVVGDFRRRDIALGGQGAPLVPAFHHALLAHPT
                     ERRMVLNIGGIANLSLLIPGQLVGGYDTGPGNMLMDAWIWRQAGKPYDKDAEWARAGK
                     VILPLLQNMLSDPYFSQPAPKSTGREYFNYGWLERHLRHFPGVDPRDVQATLAELTAV
                     TISEQVLLSGGCERLMVCGGGSRNPLLMARLAVLLLGTEVTTTDAVGISGDDMEALAF
                     AWLAWRTLAGLPGNLPSVTGASQETVLGAIFPANS"
     gene            1416..1883
                     /gene="slyB"
                     /locus_tag="OO357_001601"
     CDS             1416..1883
                     /gene="slyB"
                     /locus_tag="OO357_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein SlyB"
                     /protein_id="HCR5886587.1"
                     /translation="MIKRVLVVSMVGLSLVGCVNNDTLSGDVYTASEAKQVQNVSYGT
                     IVNVRPVQIQGGDDSNVIGAIGGAVLGGFLGNTVGGGTGRSLATAAGAVAGGVAGQGV
                     QSAMNKTQGVELEIRKDDGNTIMVVQKQGNTRFSPGQRVVLASNGSQVTVSPR"
     gene            complement(1930..2364)
                     /gene="slyA"
                     /locus_tag="OO357_001602"
     CDS             complement(1930..2364)
                     /gene="slyA"
                     /locus_tag="OO357_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416159.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SlyA"
                     /protein_id="HCR5886588.1"
                     /translation="MESPLGSDLARLVRIWRALIDHRLKPLELTQTHWVTLHNIHQLP
                     PDQSQIQLAKAIGIEQPSLVRTLDQLEEKGLISRQTCASDRRAKRIKLTEKAEPLISE
                     MEAVINKTRAEILHGISAEELEQLITLIAKLEHNIIELQAKG"
     gene            2565..2801
                     /locus_tag="OO357_001603"
     CDS             2565..2801
                     /locus_tag="OO357_001603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1656 domain-containing protein"
                     /protein_id="HCR5886589.1"
                     /translation="MKFMLNATGLPLQDLVFGASVYFPPFFKAFAFGFVIWLVVHRLL
                     RGWIYAGDIWHPLLMDLSLFAICVCLALAILIAW"
     gene            2804..3661
                     /locus_tag="OO357_001604"
     CDS             2804..3661
                     /locus_tag="OO357_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5886590.1"
                     /translation="MSIKTIKYFSTIIVAIVAVLAGWWLWNYYMQSPWTRDGKIRAEQ
                     VSITPQVSGRIVELNIKDNQLVNAGELLLTIDKTPFQIAELNAQAQLAKAQSDLAKAN
                     NEANRRRHLSQNFIFAEELDTANLNVKAMQASVNAAQATLKQTQWQLAQTEIRAPVSG
                     WVTNLTTRIGDYADTGKPLFALVDSHSFYVIGYFEETKLRHIREGAPAQITLYSDNKT
                     LQGHVSSIGRAIYDQSVESDSSLIPDVKPNVPWVRLAQLVPVRFALDKVPGDVTLVSG
                     TTCSIAVGQ"
     gene            3661..5673
                     /locus_tag="OO357_001605"
                     /pseudo
     CDS             3661..5673
                     /locus_tag="OO357_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416162.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
     gene            complement(5674..6195)
                     /gene="sodC"
                     /locus_tag="OO357_001606"
     CDS             complement(5674..6195)
                     /gene="sodC"
                     /locus_tag="OO357_001606"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Cu-Zn] SodC2"
                     /protein_id="HCR5886591.1"
                     /translation="MKRFSLAILALVVATGAQAASEKVEMNLVTSQGVGQSIGSVTIT
                     ETDKGLEFSPDLKALPPGEHGFHIHAKGSCQPATKDGKASAAESAGGHLDPQNTGKHE
                     GPEGTGHLGDLPALVVNNDGKATDAVIAPRLKSLDEIKDKALMVHVGGDNMSDQPKPL
                     GGGGERYACGVIK"
     gene            complement(6276..7172)
                     /locus_tag="OO357_001607"
     CDS             complement(6276..7172)
                     /locus_tag="OO357_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase family oxidoreductase"
                     /protein_id="HCR5886592.1"
                     /translation="MVQRITIAPEGPEFSRFVMGYWRLMDWNMSARQLVSFIEEHLDL
                     GVTTVDHADIYGGYQCEAAFGEALKLAPHLRERMEIVSKCGIATTAREENVIGHYITD
                     RNHIIKSAEQSLINLATDHLDLLLIHRPDPLMDADEVADAFKHLHQSGKVRHFGVSNF
                     TPAQFALLQSRLPFTLATNQVEISPVHQPLLLDGTLDQLQQLRVRPMAWSCLGGGRLF
                     NDDYFQPLRDELAVVAEELNAGSIEQVVYAWVLRLPSQPLPIIGSGKIERVRAAVEAE
                     TLKMTRQQWFRIRKAALGYDVP"
     gene            complement(7221..7460)
                     /locus_tag="OO357_001608"
     CDS             complement(7221..7460)
                     /locus_tag="OO357_001608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HCR5886593.1"
                     /translation="MAEQLEFFPVQSPCRGICQSDERGFCRGCFRSRDERFNWNKMSD
                     GEKQEVLRLCRQRLMRKLRANKPASSDEPEQPSLF"
     gene            7563..8162
                     /gene="nemR"
                     /locus_tag="OO357_001609"
     CDS             7563..8162
                     /gene="nemR"
                     /locus_tag="OO357_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707550.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NemR"
                     /protein_id="HCR5886594.1"
                     /translation="MNKHTEHDTREHLLATGEQLCLQRGFTGMGLSELLKTAEVPKGS
                     FYHYFRSKEAFGVAMLERHYAAYHQRLAELLQSGEGNYRDRILAYYQQTLNQFCQHGT
                     ISGCLTVKLSAEVCDLSEDMRSAMDKGARGVIALLSQALENGRENHCLTFCGEPLQQA
                     QVLYALWLGANLQAKISRSFEPLENALAHVKNIIATPAV"
     gene            8199..9296
                     /gene="nemA"
                     /locus_tag="OO357_001610"
     CDS             8199..9296
                     /gene="nemA"
                     /locus_tag="OO357_001610"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-ethylmaleimide reductase"
                     /protein_id="HCR5886595.1"
                     /translation="MSSEKLYSPLKVGAITAANRIFMAPLTRLRSIEPGDIPTPLMAE
                     YYRQRASAGLIISEATQISAQAKGYAGAPGIHSPEQIAAWKKITAGVHAENGHMALQL
                     WHTGRISHASLQPGGQAPVAPSALSAGTRTSLRDENGQAIRVETSMPRALELEEIPGI
                     VNDFRQAIANAREAAFDLVELHSAHGYLLHQFLSPSSNHRTDQYGGSVENRARLVLEV
                     VDAGIEEWGADRIGIRVSPIGTFQNTDNGPNEEADALYLIEQLGKRGIAYLHMSEPDW
                     AGGEPYTDAFREKVRARFHGPIIGAGAYTVEKAETLIGKGLIDAVAFGRDWIANPDLV
                     ARLQLKAELNPQRAESFYGGGAEGYTDYPTL"
     gene            9377..9784
                     /gene="gloA"
                     /locus_tag="OO357_001611"
     CDS             9377..9784
                     /gene="gloA"
                     /locus_tag="OO357_001611"
                     /EC_number="4.4.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HCR5886596.1"
                     /translation="MRLLHTMLRVGDLQRSIDFYTKVLGMKLLRTSENPEYKYSLAFV
                     GYGPETEEAVIELTYNWGVDKYELGTAYGHIALSVDNAAEACEKIRQNGGNVTREAGP
                     VKGGTTVIAFVEDPDGYKIELIEEKDAGRGLGN"
     gene            9887..10534
                     /gene="rnt"
                     /locus_tag="OO357_001612"
     CDS             9887..10534
                     /gene="rnt"
                     /locus_tag="OO357_001612"
                     /EC_number="3.1.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HCR5886597.1"
                     /translation="MSDNAQLTGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITL
                     KMDEQGWLMPDTTLHFHVEPFVGANLQPEALAFNGIDPNDPDRGAVSEYEALHEIFKV
                     VRKGIKASGCNRAIMVAHNANFDHSFMMAAAERASLKRNPFHPFATFDTAALAGLALG
                     QTVLSKACQTAGMDFDSTQAHSALYDTERTAVLFCEIVNRWKRLGGWPLPAAEEV"
     gene            10627..12741
                     /locus_tag="OO357_001613"
     CDS             10627..12741
                     /locus_tag="OO357_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000774812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HCR5886598.1"
                     /translation="MADNPDPSSLLPDVFSPATRDWFLRAFKQPTAVQSQTWHVAARG
                     EHALVIAPTGSGKTLAAFLYALDRLFREGGEDTREAHKRKTSRILYISPIKALGTDVQ
                     RNLQIPLKGIADERRRRGETVIIDEVHAVAGSKRGAHLALSLERLDALLHTSAQRIGL
                     SATVRSASDVAAFLGGDRPVTVVNPPTMRHPQIRIVVPVANMDDVSSVASGTGEDSHA
                     GREGSIWPYIETGILDEVLRHRSTIVFTNSRGLAEKLTARLNELYAARLQRSPSIAVD
                     AAHFESTSGATSNRVQSSDVFIARSHHGSVSKEQRAITEQALKSGELRCVVATSSLEL
                     GIDMGAVDLVIQVATPLSVASGLQRIGRAGHQVGGVSKGLFFPRTRRDLVDSAVIVEC
                     MFAGRLENLTPPHNPLDVLAQQTVAAAAMDALQVDEWYSRVRRTAPWKDLPRRVFDAT
                     LDMLSGRYPSGDFSAFRPKLVWNRETGILTARPGAQLLAVTSGGTIPDRGMYSVLLPE
                     GEEKAGSRRVGELDEEMVYESRVNDIITLGATSWRIQQITRDQVIVTPAPGRSAQLPF
                     WRGEGNGRPAELGEMIGDFLHLLADGAFFSGTIPPWLAEENTNANIQGLIDEQRNATG
                     IVPGSRHLVLERCRDEIGDWRIILHSPYGRRVHEPWALAIAGRIHALWGADASVVASD
                     DSIVARIPDTDGKLPDAAIFLF"
     gene            12751..14043
                     /locus_tag="OO357_001614"
     CDS             12751..14043
                     /locus_tag="OO357_001614"
                     /inference="COORDINATES: protein motif:HMM:NF020083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886599.1"
                     /translation="MLQIVREAVGSSALFAARFRECAARALLMPGRTPGHRTPLWQQR
                     LRASQLLEIAQGYPDFPVILETLRECLQDVYDLPALERLMRRLNGGEIQISDVTTTTP
                     SPFATSLLFGYVAEFMYQSDAPLAERRASVLSLDSELLRNLLGQVDPGELLDPQVIRQ
                     VEEELQRLAPGRRAKGEEGLFDLLRELGPMTVEDLAQRHTGSSEEVASYLENLLAVKR
                     IFPAMISGQERLACMDDAARLRDALGVRLPESLPEIYLHRVSYPLRDLFLRYLRAHAL
                     VTAEQLAHEFSLGIAIVEEQLQQLREQGLVMNLQQDIWVSDEVFRRLRLRSLQAAREA
                     TRPVAATTYARLLLERQGVLPATDGSPALFASTSPGVYEGVDGVMRVIEQLAGVGLPA
                     SLWESQILPARVRDYSPEMLDELLATGAVIWSGQKKAG"
     gene            14993..15085
                     /locus_tag="OO357_001615"
     CDS             14993..15085
                     /locus_tag="OO357_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001441976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HCR5886600.1"
                     /translation="MNDLPVRQTPMFTLLREAGFSSSPQGLDWG"
     gene            complement(15129..16337)
                     /locus_tag="OO357_001616"
     CDS             complement(15129..16337)
                     /locus_tag="OO357_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5886601.1"
                     /translation="MKRLQAFKFQLRPGGQQEREMRRFAGACRFVFNRALALQNENHE
                     AGNKYIPYGKMASWLVEWKNATETQWLKDSPSQPLQQSLKDLERAYKNFFQKRAAFPR
                     FKKRGQNAAFRYPQDVKLDQENSRIFLPKLGWMRYRNSRQVTGVVKNVTVSQSCGTWY
                     ISIQTESEVSTPVHPSASMIGLDAGVAKLATLSDGTVFEPVNSFQKNQKTLARLQRQL
                     SRKVKFSNNWQKQKRKIQRLHSCIANIRRDYLHKVTTTVSKNHAMIVIEDLKVSNMSK
                     SAAGTVSQPGRNVRAKSGLNRSILDQGWYEMRRQLEYKQLWSGGQVLAVPPAYTSQRC
                     ACCGHTAKENRLSQSKFRCQVCGYTANADVNGARNILAAGHAVLACGEMVQSGRSLKQ
                     EPTEMIQATA"
     gene            16345..16845
                     /gene="tnpA"
                     /locus_tag="OO357_001617"
     CDS             16345..16845
                     /gene="tnpA"
                     /locus_tag="OO357_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HCR5886602.1"
                     /translation="MILWSVKKETDIRRGRHCVFLMHVHLVFVTKYRRQIFDHDTTEK
                     LRTYFSNVCADFAAELVEMDGEPDHVHLLINYPPKLAISSLVNSLKGISNRLLRRDRP
                     DIAVRYYYKGVLWSPGYFASSCEGAPISVIRQYIEQQQTPGQVENRALYPRPEGRGFT
                     AHRIKD"
     gene            complement(16884..17231)
                     /gene="grxD"
                     /locus_tag="OO357_001618"
     CDS             complement(16884..17231)
                     /gene="grxD"
                     /locus_tag="OO357_001618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monothiol glutaredoxin 4"
                     /protein_id="HCR5886603.1"
                     /translation="MSTTIEKIQRQIAENPILLYMKGSPKLPSCGFSAQAVQALAACG
                     ERFAYVDILQNPDIRAELPKYANWPTFPQLWVDGELVGGCDIVIEMYQRGELQQLIKE
                     TAAKYKSEEPDAE"
     gene            17566..18393
                     /gene="mepH"
                     /locus_tag="OO357_001619"
     CDS             17566..18393
                     /gene="mepH"
                     /locus_tag="OO357_001619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707555.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-endopeptidase MepH"
                     /protein_id="HCR5886604.1"
                     /translation="MARINRISITLCALLFTTLPLTPMAHASKQARESSATTHITKKA
                     DKKKSTATTKKTQKASQKTAKKAASKSMTKSKTASSVKKSSITASKNAKTRSKHAVNK
                     TASASFTEKCTKRKGYKSHCVKVKNAASGTLADAHKAKVQKATKVAMNKLMQQIGKPY
                     RWGGSSPRTGFDCSGLVYYAYKDLVKIRIPRTANEMYHLRDAGPIERSELKNGDLVFF
                     RTQGRGTADHVGVYVGNGKFIQSPRTGQEIQITSLSEDYWQRHYVGARRVMTPKTLR"
     gene            18521..19102
                     /gene="sodB"
                     /locus_tag="OO357_001620"
     CDS             18521..19102
                     /gene="sodB"
                     /locus_tag="OO357_001620"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Fe]"
                     /protein_id="HCR5886605.1"
                     /translation="MSFELPALPYAKDALAPHISAETIEYHYGKHHQTYVTNLNNLIK
                     GTAFEGKSLEEIIRSSEGGVFNNAAQVWNHTFYWNCLAPNAGGEPTGKVAEAIAASFG
                     SFADFKAQFTDAAIKNFGSGWTWLVKNSDGKLAIVSTSNAGTPLTTDATPLLTVDVWE
                     HAYYIDYRNARPGYLEHFWALVNWEFVAKNLAA"
     gene            complement(19248..20417)
                     /locus_tag="OO357_001621"
     CDS             complement(19248..20417)
                     /locus_tag="OO357_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5886606.1"
                     /translation="MKINYPLLALAIGAFGIGTTEFSPMGLLPVIARGVDVSIPAAGM
                     LISAYAVGVMVGAPLMTLLLSHRARRSALIFLMAIFTLGNVLSAIAPDYMTLMLSRIL
                     TSLNHGAFFGLGSVVAASVVPKHKQASAVATMFMGLTLANIGGVPAATWLGETIGWRM
                     SFLATAGLGVISMVSLFFSLPKGGTGARPEVKKELAVLMRPQVLSALLTTVLGAGAMF
                     TLYTYISPVLQSITHATPVFVTAMLVLIGVGFSIGNYLGGKLADRSVNGTLKGFLLLL
                     MVIMLAIPFLARNEFGAAISMVVWGAATFAVVPPLQMRVMHVASEAPGLSSSVNIGAF
                     NLGNALGAAAGGAVISAGLGYSFVPVTGAIVAGLALLLVFMSARKQPETVCVANS"
     gene            complement(20583..20672)
                     /gene="ynhF"
                     /locus_tag="OO357_001622"
     CDS             complement(20583..20672)
                     /gene="ynhF"
                     /locus_tag="OO357_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein YnhF"
                     /protein_id="HCR5886607.1"
                     /translation="MSTDLKFSLVTTVIVLGLIVAVGLTAALH"
     gene            20971..21996
                     /gene="purR"
                     /locus_tag="OO357_001623"
     CDS             20971..21996
                     /gene="purR"
                     /locus_tag="OO357_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor PurR"
                     /protein_id="HCR5886608.1"
                     /translation="MATIKDVAKRANVSTTTVSHVINKTRFVAEETRNAVWAAIKELH
                     YSPSAVARSLKVNHTKSIGLLATSSEAAYFAEIIEAVEKNCFQKGYTLILGNAWNNLE
                     KQRAYLSMMAQKRVDGLLVMCSEYPEPLLAMLEEYRHIPMVVMDWGEAKADFTDAVID
                     NAFEGGYMAGRYLIERGHREIGVIPGPLERNTGAGRLAGFMKAMEEAMIKVPESWIVQ
                     GDFEPESGYRAMQQILSQPHRPTAVFCGGDIMAMGALCAADEMGLRVPQDVSLIGYDN
                     VRNARYFTPALTTIHQPKDSLGETAFNMLLDRIVNKREEPQSIEVHPRLIERRSVADG
                     PFRDYRR"
     gene            complement(21993..22925)
                     /locus_tag="OO357_001624"
     CDS             complement(21993..22925)
                     /locus_tag="OO357_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5886609.1"
                     /translation="MWSEYSLEVVDAVARNGSFSAAAQELHRVPSAVSYTVRQLEEWL
                     AVPLFERRHRDVELTAAGAWFLKEGRSVVKKMQITRQQCQQIANGWRGQLAIAVDNIV
                     RPERTRQMIVDFYRHFDDVELLVFQEVFNGVWDALSDGRVELAIGATRAIPVGGRYAF
                     RDMGMLSWSCVVASHHPLALMDGPFSDDTLRNWPSLVREDTSRTLPKRITWLLDNQKR
                     LVVPDWESSATCISAGLCIGMVPTHFAKPWLNEGKWVALELENPFPDSACCLTWQQND
                     MSPALTWLLEYLSDSETLNKEWLREPEETPATGD"
     gene            23038..24249
                     /locus_tag="OO357_001625"
     CDS             23038..24249
                     /locus_tag="OO357_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bcr/CflA family multidrug efflux MFS
                     transporter"
                     /protein_id="HCR5886610.1"
                     /translation="MQPGKRFLVWLAGLSVLGFLATDMYLPAFAAIQADLQTPASAVS
                     ASLSLFLAGFAAAQLLWGPLSDRYGRKPVLLIGLTIFALGSLGMLWVENAATLLVLRF
                     VQAVGVCAAAVIWQALVTDYYPSQKVNRIFATIMPLVGLSPALAPLLGSWLLVHFSWQ
                     AIFATLFAITVVLILPIFWLKPTTKARNNSQDGLTFTDLLRSKTYRGNVLIYAACSAS
                     FFAWLTGSPFILSEMGYSPAVIGLSYVPQTIAFLIGGYGCRAALQKWQGKQLLPWLLV
                     LFAVSVIATWAAGFISHVSLVEILIPFCVMAIANGAIYPIVVAQALRPFPHATGRAAA
                     LQNTLQLGLCFLASLVVSWLISISTPLLTTTSVMLSTVVLVALGYMMQRCEEVDCPNH
                     GNAEVAHSESH"
     gene            24540..25688
                     /gene="cfa"
                     /locus_tag="OO357_001626"
     CDS             24540..25688
                     /gene="cfa"
                     /locus_tag="OO357_001626"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HCR5886611.1"
                     /translation="MSSSCIEEVSVPDDNWYRIANELLSRAGIAINGSAPADIRVKNP
                     DFFKRVLQEGSLGLGESYMDGWWECDRLDMFFSKVLRAGLENQLPHHFKDTLRIAGAR
                     LFNLQSKKRAWIVGKEHYDLGNDLFSRMLDPFMQYSCAYWKDADNLESAQQAKLKMIC
                     EKLQLKPGMRVLDIGCGWGGLAHYMASNYDVSVVGVTISAEQQKMAQERCKGLDVTIL
                     LQDYRDLNDQFDRIVSVGMFEHVGPKNYDTYFAVVDRNLKPEGIFLLHTIGSKKTDLN
                     VDPWINKYIFPNGCLPSVRQIAQSSEPHFVMEDWHNFGADYDTTLMAWYERFLAAWPE
                     IADNYSERFKRMFTYYLNACAGAFRARDIQLWQVVFSRGVENGLRVAR"
     gene            complement(25728..26369)
                     /gene="ribE"
                     /locus_tag="OO357_001627"
     CDS             complement(25728..26369)
                     /gene="ribE"
                     /locus_tag="OO357_001627"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HCR5886612.1"
                     /translation="MFTGIVQGTAKLVSIDEKPNFRTHVVELPDHMLDGLETGASVAH
                     NGCCLTVTEINGNHVSFDLMKETLRITNLGDLKVGDWVNVERAAKFSDEIGGHLMSGH
                     IMTTAEVAKILTSENNRQIWFKVQDSQLMKYILYKGFIGIDGISLTVGEVTPTRFCVH
                     LIPETLERTTLGKKKLGARVNIEIDPQTQAVVDTVERVLAARENAMNQPGTEA"
     gene            26584..27957
                     /gene="mdtK"
                     /locus_tag="OO357_001628"
     CDS             26584..27957
                     /gene="mdtK"
                     /locus_tag="OO357_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MATE transporter MdtK"
                     /protein_id="HCR5886613.1"
                     /translation="MQKYISEARLLLALAIPVILAQIAQTAMGFVDTVMAGGYSATDM
                     AAVAIGTSIWLPAILFGHGLLLALTPVIAQLNGSGRRERIAHQVRQGFWLAGFVSVLI
                     MLVLWNAGYIIRYMENIDPALADKAVGYLRALLWGAPGYLFFQVARNQCEGLAKTKPG
                     MVMGFIGLLVNIPVNYIFIYGHFGMPELGGVGCGVATAAVYWVMFLAMVSYIKRARSM
                     RDIRNEKGTAKPEPAVMKRLIQLGLPIALALFFEVTLFAVVALLVSPLGIVDVAGHQI
                     ALNFSSLMFVLPMSLAAAVTIRVGYRLGQGSTLDAQTAARTGLMVGVCMATLTAIFTV
                     SLREQIALLYNDNPEVVTLAAHLMLLAAVYQISDSIQVIGSGILRGYKDTRSIFYITF
                     TAYWVLGLPSGYILALTDLVVEPMGPAGFWIGFIIGLTSAAIMMMLRMRFLQRMPSAI
                     ILQRASR"
     gene            complement(27998..29254)
                     /locus_tag="OO357_001629"
     CDS             complement(27998..29254)
                     /locus_tag="OO357_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AIDA repeat-containing protein"
                     /protein_id="HCR5886614.1"
                     /translation="MGSDAKNLMSDGNVQIVKTGEVLGATQLTEGELIVEAGGRAENT
                     VVTGAGWLKVATGGIAKCTQYGNNGTLSVSDGAIATDIVQSEGGAISLSTLATVNGRH
                     PEGEFSVDQGYACGLLLENGGNLRVLEGHRAEKIILDQEGGLLVNGTTSAVVVDEGGE
                     LLVYPSGEASNCEINQGGVFMLAGKASDTLLAGGTMNNLGGEDSDTIVENGSIYRLGT
                     DGLQLYSSGKTQNLSVNVGGRAEVHAGTLENAVIQGGTVILLSPTSADENFVVEEDRA
                     PVELTGSVALLDGASMIIGYGAELQQSTITVQQGGVLILDGSTVKGDSVTFIVGNINL
                     NGGKLWLITGAATHVQLKVKRLRGEGAICLQTSAKEISPDFINVKGEVNGDIRVEITD
                     ASRQTLCNALKLQPDEDGIGATLQPA"
     gene            29562..29638
                     /locus_tag="OO357_001630"
     tRNA            29562..29638
                     /locus_tag="OO357_001630"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29596..29598,aa:Val,seq:gac)
     gene            29643..29719
                     /locus_tag="OO357_001631"
     tRNA            29643..29719
                     /locus_tag="OO357_001631"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:29677..29679,aa:Val,seq:gac)
     gene            29827..30132
                     /locus_tag="OO357_001632"
     CDS             29827..30132
                     /locus_tag="OO357_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="monooxygenase"
                     /protein_id="HCR5886615.1"
                     /translation="MATLLQLHFAFNGPFGDAMAEQLKPLAESINQEPGFLWKVWTES
                     EKNHEAGGIYLFTDEKSALAYLEKHTARLKNLGVEEVVAKVFDVNEPLSQINQAKLA"
     gene            30258..31680
                     /locus_tag="OO357_001633"
                     /pseudo
     CDS             30258..31680
                     /locus_tag="OO357_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-NAD(P)-binding protein"
     gene            complement(31692..32504)
                     /locus_tag="OO357_001634"
     CDS             complement(31692..32504)
                     /locus_tag="OO357_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886616.1"
                     /translation="MIITRADLREWRIGAVMYRWFLRHFPRGGSYADIRHALIEEGYT
                     DWAESLVEYAWKKWLADENFAHQEVSSMQKLATDPGEIPFCSQFARSDDHARIGCCED
                     NARIATAGYAAQIASMGYSVRIGSVGFNSHIGSSGERARVAVTGNSSRISSAGDSSRI
                     ANTGMRVRVCTLGERCHVASNGDLVQIASFGANARIANSGDNVHIIASGENSTVVSTG
                     VVDSIILGLGGSAALAYHDGERVRFAVAIEGENNIRTGVRYRLNEQHQFVEC"
     gene            complement(32508..33293)
                     /locus_tag="OO357_001635"
     CDS             complement(32508..33293)
                     /locus_tag="OO357_001635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate reductase cytochrome B subunit"
                     /protein_id="HCR5886617.1"
                     /translation="MNPSQHAEQFQSQLANYVPQFTPEFWPVWLIIAGVLLVGMWLVL
                     GLHALLRARGVKKSATDHGEKVYLYSKAVRLWHWSNALLFVLLLASGLINHFAMVGAT
                     AVKSLVAVHEVCGFLLLACWLGFVLINAVGDNGHHYRIRRQGWLERAAKQTRFYLFGI
                     MQGEEHPFPATTQSKFNPLQQVAYVGVMYGLLPLLLLTGLLCLYPQAVGDVFPGVRYW
                     LLQAHFALAFISLFFIFGHLYLCTTGRTPHETFKSMVDGYHRH"
     gene            complement(33290..33958)
                     /locus_tag="OO357_001636"
     CDS             complement(33290..33958)
                     /locus_tag="OO357_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416186.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HCR5886618.1"
                     /translation="MSFTRRKFVLGMGTVIFFTGSASSLLANTRQEKEVRYAMIHDES
                     RCNGCNICARACRKTNHVPAQGSRLSIAHIPVTDNDNETQYHFFRQSCQHCEDAPCID
                     VCPTGASWRDEQGIVRVEKSQCIGCSYCIGACPYLVRYLNPVTKVADKCDFCAESRLA
                     KGFPPICVSACPEHALIFGREDSPEIQAWLQDNKYYQYQLPGAGKPHLYRRFGQHLIK
                     KENV"
     gene            complement(34022..34660)
                     /locus_tag="OO357_001637"
     CDS             complement(34022..34660)
                     /locus_tag="OO357_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707570.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdhW family putative oxidoreductase system
                     protein"
                     /protein_id="HCR5886619.1"
                     /translation="MNHQDELPLAKVSEVDEAKRQWLQGMRHPVDTVTEPEPAEILAE
                     FIRQHSAAGQLVARTVFLSPPYSVAEEELSVLLESIKQNGDYADIACMTGSQDDYYYS
                     TQAMSENYAAMSLQVVEQDICRAIAHAVRFECQTYPRPYKVAMLMQAPYYFQEAQIEA
                     AIAAMDVAPEYADIRQVESSTAVLYLFSERFMTYGKAYGLCEWFEVEQFQNP"
     gene            complement(34673..36752)
                     /locus_tag="OO357_001638"
                     /pseudo
     CDS             complement(34673..36752)
                     /locus_tag="OO357_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416188.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase"
     gene            complement(36773..37399)
                     /locus_tag="OO357_001639"
     CDS             complement(36773..37399)
                     /locus_tag="OO357_001639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like protein"
                     /protein_id="HCR5886620.1"
                     /translation="MNPVDRPLLDIGLTRLEFLRISGKGLAGLTIAPALLSLLGCKQE
                     DIDSGTVGLINTPKGVLVTQRARCTGCHRCEISCTNFNDGSVGTFFSRIKIHRNYFFG
                     DNGVGSGGGLYGDLNYTADTCRQCKEPQCMNVCPIGAITWQQKEGCITVDHKRCIGCS
                     ACTTACPWMMATVNTESKKSSKCVLCGECANACPTGALKIIEWKDITV"
     gene            complement(37855..38064)
                     /gene="fumD"
                     /locus_tag="OO357_001640"
     CDS             complement(37855..38064)
                     /gene="fumD"
                     /locus_tag="OO357_001640"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase FumD"
                     /protein_id="HCR5886621.1"
                     /translation="MGNRTKEDELYREMCRVVGKVVLEMRDLGQEPKHIVIAGVLRTA
                     LANKRIQRSELEKQAMETVINALVK"
BASE COUNT         9159 a         9954 c         9332 g         9644 t
ORIGIN      
        1 cacgataaga aaaaattcag ttaattctga tatcacgagt tggcggggaa aatagccccc
       61 agtaccgtct cctggcttgc gccagtgacg gaaggcagat ttcctggtaa tcccgccagc
      121 gtccgccagg caagccaggc gaaagccaat gcttccatgt catcgccact aatgccaacg
      181 gcatcggtgg tggtgacttc tgtgcctagc agtaataccg ccagacgcgc catgagtagc
      241 ggattacgac tacctccacc acataccatc aatcgttcgc agccaccgct caacaaaact
      301 tgttcagaaa tggtcacggc ggtgagttct gccagtgtgg cctgaacatc acggggatca
      361 acacccggaa aatggcgtaa atggcgctcc agccagccgt agttaaagta ttcgcgtccg
      421 gtgcttttcg gtgcaggttg cgagaaatac gggtcgctga gcatattttg cagcagtggg
      481 agaataactt tacccgcccg tgcccactcg gcatctttat cgtaaggttt acctgcctga
      541 cgccagatcc aggcatccat cagcatgtta ccaggaccgg tatcgtagcc gccaaccagc
      601 tgcccaggaa tgagcagtga cagattggcg atgccgccaa tattgagcac cattcgtcgc
      661 tcggttgggt gagccagcaa ggcatgatgg aacgcaggta ccagcggtgc gccttgtcct
      721 cccaaggcaa tatcgcgacg gcgaaaatca ccgacaaccg taattccggt gcgtgccaca
      781 atttgattgt tatcgccaat ctgtaaagtg tgtggtgcca cgcccgtcgg ttcatgccag
      841 acggtttgac cgtgacagcc gatcgcaact atatctctcg cctgcaggtt ttgttcctta
      901 agcaaggcat tgaccgcatc agcaaaaagt cgcccgagtt gagtatcaag ctgtccaaac
      961 tgcgaaagtg taagctgctg gccctggcaa atatccagta cagcctgttt cagagacacc
     1021 gggatcggcc agctcaagct tgccagctgt gcgacccggt gttcatcaat tgtcgccaac
     1081 acaacatcaa caccatcaag gctggtgcct gacataacgc caataaagcg gcccgatttc
     1141 atagttcatc ctttttcaat ctgacgtttg cgcaccactc aaacataaac ttttcgtgaa
     1201 taccatgcgg aatgaccgat ttttaccgtt ggtagtaaaa cattatcttc aaatcaataa
     1261 tcatcatgaa tgttttgttt ataattggtt gatcctactt tcattatgat ttgctcatat
     1321 ttggtagaac atgtaaccat ggattcacat atgccatata ctttgaccat gagggatgct
     1381 tgcgtggcgt ttcatggtga acaggagatt tttcaatgat taaacgcgta ttggttgttt
     1441 caatggtagg tctgtctctt gtcggttgtg ttaataacga caccctgtca ggggatgttt
     1501 ataccgcttc tgaagcgaaa caagtacaga atgtcagcta tggcaccatc gttaacgtac
     1561 gtccggtaca gattcagggc ggtgatgatt ccaacgttat cggtgcaatt ggcggtgctg
     1621 ttcttggtgg tttcctgggg aatactgttg gtggcggaac cgggcgttct ctggctactg
     1681 cagcaggcgc tgttgcaggt ggcgtagctg gtcagggcgt acagagtgca atgaacaaaa
     1741 cgcagggtgt cgagctggaa attcgtaaag acgatggtaa taccatcatg gtggtacaga
     1801 aacaaggcaa cactcgtttc tctccgggcc aacgtgtcgt actggccagc aatggcagtc
     1861 aggtgaccgt ttctccgcgc taaataagtt tgcgtgtggt caggttactg accacacgcc
     1921 cccttcattt caccctttgg cctgtaactc aatgatatta tgctcaagtt ttgcgatgag
     1981 cgtaatcagt tgctccagtt cctctgcgga gatgccatgt aatatttccg cgcgggtttt
     2041 gttaataaca gcttccattt cgctgatcag tggctctgcc ttttccgtca gtttaatacg
     2101 tttagcccga cgatcgctgg cacaagtttg acgcgaaatt aacccttttt cttccagttg
     2161 gtccagagta cggaccagtg atggctgctc gatgccaatc gcttttgcca gttgaatttg
     2221 cgactggtct ggaggtaact gatggatatt gtgtaacgta acccaatggg tttgtgttaa
     2281 ctccagcggt ttcaggcgat ggtctatcag agcacgccat atgcgcacca accgtgccag
     2341 atcagaacct agtggcgatt ccaatttcat ctccttataa ttagcttgct aagatattat
     2401 gcggctttta gaatagtgtg cagcaattgt attgctaaaa caaatgtatt gctgcatttg
     2461 gttaccgtca gacatatttt tcagaaattg cgcgtaaatt tttcgcactt aaagaatatt
     2521 tattaatcta acgcaatata ttcggtcgta aaggaatcta ctttgtgaag tttatgctca
     2581 atgcaacagg attgccctta caagatctgg tgttcggtgc gtccgtctat tttcctccgt
     2641 ttttcaaagc attcgcgttt ggattcgtca tctggcttgt cgtacaccgc ctgcttcgtg
     2701 gctggatcta cgccggcgat atctggcatc ccttgttaat ggatttatcg ctgtttgcga
     2761 tttgcgtttg ccttgctctg gcaatactga ttgcgtggta actatgtcaa ttaaaacaat
     2821 taagtatttc tcaacaatca ttgtagcgat agttgcggtt cttgccggat ggtggctgtg
     2881 gaattattac atgcaatctc cgtggacacg cgacgggaaa atacgcgcag aacaggtttc
     2941 tatcacccca caggtgtcgg gacgtattgt tgagctgaat ataaaagaca accaactggt
     3001 taacgcgggg gaacttctgc tcacgatcga taaaacgccc tttcagatcg ccgagctgaa
     3061 cgcacaggct cagctggcga aagcgcaatc tgacctcgca aaagccaata acgaagcgaa
     3121 tcgtcgtcgt catctctcac aaaattttat ttttgccgaa gaactcgata ccgctaacct
     3181 caatgttaaa gcgatgcagg ccagtgttaa tgccgcacag gcgacgctga aacagacaca
     3241 atggcaactg gcgcaaacgg aaattcgcgc cccggtgagt ggatgggtga ctaacctcac
     3301 tacccgcatc ggcgattacg ctgacaccgg gaaaccgctg tttgcccttg tcgacagcca
     3361 ctcgttttac gtcattggtt attttgaaga aaccaagttg cgccatatcc gcgaaggtgc
     3421 accagcacaa attaccctct atagtgataa caaaacgtta cagggtcacg tttccagtat
     3481 cggtcgggcg atttatgatc agagcgttga aagtgactca agccttattc cggatgtgaa
     3541 acctaacgtc ccctgggttc gtctcgccca actcgttccc gtccgctttg cgctggataa
     3601 agttccaggc gatgtcacgc tggtgtcggg caccacctgt agcattgccg taggtcaata
     3661 atgaacgcat cgtcatggtc cttgcgcaat ttgccctggt tcagggccac gctggcgcaa
     3721 tggcgttatg cgttacgcaa taccattgcc atgtgtctgg cgctgacggt tgcctattat
     3781 ttgaatctgg atgaacccta ttgggcgatg acctcggctg cagtggttag ctttcccacc
     3841 gttggcggtg tcatcagcaa aagcctcgga cgcatggctg gcagtttact gggagccatt
     3901 gcggcactgc ttcttgccgg acatacgctc aatgagccgt ggttttttct attgagcatg
     3961 tcggcgtggc ttggcttttg tacctaggcc tgtgcgcact tcacgaataa cgtcgcgtat
     4021 gcatttcaac tggcgggcta cacggctgcc atcatcgcct ttccgatggt taatattact
     4081 gaggccagcc agctgtggga tatcgctcag gcgcgcgttt gcgaggtgat agtcggtatt
     4141 ttgtgcggcg gcatgatgat gatgatcctg cctagcagtt ccgatgctac tgccctttta
     4201 actgcattga aaaacatgca cgcccgatta ctggaacatg ccagtttact ctggcagcct
     4261 gaaacaaacg atgccattcg tgcagcacat gagggggtga ttgggcagat actgaccatg
     4321 aatttgctgc gtatccaggc tttctggagc cactatcgtt ttcgccagca aaacgcgcgc
     4381 cttaatgcgc tactccacca gcaattacgt atgaccagtg tcatctccag cctgcgacgt
     4441 atgttgctca actggccctc accgccaggt gccacgcgag aaattctcga acaattgctg
     4501 acggcgctcg ccagttcgca aacagatgct tacaccgtcg cacgtattat cgccccgcta
     4561 cgcccgacca acgtcgccga ctaccggcac gtcgccttct ggcagcgact acgttatttt
     4621 tgccgccttt atctgcaaag tagtcaggaa ttacatcgtc tgcaaagcgg tgtagatgat
     4681 catgccagac tcgcacggac atccgctctg gctcgtcata ccgacaacgc cgaagctatg
     4741 tggagcgggc tgcgtacatt ttgtacgttg atgatgattg gcgcatggag tattgcatcg
     4801 caatgggatg ccggtgccaa tgcattaacg ctggcagcaa ttagctgcgt actctactcc
     4861 gccgtcgcag caccgtttaa gtcgttgtca ctgctgatgc gcacgctggt gttactttcg
     4921 ctattcagct ttgtggtcaa atttggcctg atggtccaga ttagcgatct gtggcaattt
     4981 ttactgtttc tctttccact gctggcgaca atgcagcttc ttaaattgca gatgcccaaa
     5041 tttgccgcac tgtgggggca actgattgtt tttatgggtt cgtttatcgc tgtcactaat
     5101 cccccggtgt atgattttgc tgattttctt aactataatc tggcaaaaat cgttggcgtt
     5161 gcgctggctt ggctagcgtt cgccattctg cgtccaggat cggatgctcg taaaagccgc
     5221 cgccatattc gcgcgctgcg ccgggatttt gtcgatcagc taagccgcca tccaacactg
     5281 agtgaaagcg aatttgaatc gctcacttat catcacgtca gtcagttgag taacagccag
     5341 gatgcgctgg ctcgccgttg gttattacgc tggggtgtag tgctgctgaa ctgttctcat
     5401 gttgtctggc aattgcgcga ctgggaatcg cgttccgatc cgttatcgcg agtacgggat
     5461 aactgtattt cactgttgcg gggagtgatg agtgagcgtg gcgttcagca aaaatcactg
     5521 gcggccacac ttgaagaatt acagcggatt tgcgacagcc ttgcccgtca tcatcaacct
     5581 gccgcccgtg agctggcggc aattgtctgg cggctgtact gctcgctttc gcaacttgag
     5641 caagcaccac cgcaaggtac gctggcctcg taattactta attacaccac aggcatagcg
     5701 ttcaccgcca ccgcccagcg gtttaggttg atcggacata ttatcgccgc caacgtggac
     5761 catcagcgcc ttgtctttga tttcatccag tgatttcaga cgaggcgcga tgacggcatc
     5821 ggtagctttg ccgtcattat tgacgaccag tgcaggcaga tcgcctaaat gcccggtacc
     5881 ttctggccct tcatgtttac cggtattttg tggatcaaga tgcccgcctg cggattccgc
     5941 ggcgctggct ttgccatctt tggtggctgg ctggcagctt cctttggcat gaatatggaa
     6001 gccatgttca ccggggggta atgctttcag atcgggtgaa aactccagac ctttatcggt
     6061 ttcagtaatg gtgacgctac caattgactg cccaacccct tgcgacgtga cgaggttcat
     6121 ctcgactttt tcactggcag cttgtgcgcc ggtcgcaaca accagcgcca gaatggccag
     6181 actaaaacgt ttcataggac ctccgttcat tgtgcattcc tgaataagtg taaaccagtg
     6241 acacattttt gaacggcggc tattcctaaa agtgcttacg gtacgtcgta ccccagtgca
     6301 gctttacgga tacgaaacca ttgttgacgg gtcattttca gtgtttctgc ttcgacagct
     6361 gcccgtacgc gctcaatttt acctgagccg ataattggca gcggctgcga cggtaaacgt
     6421 aatacccagg cgtaaaccac ctgttcaatc gagcccgcgt ttaactcctc cgccaccaca
     6481 gccagttcat cacgcagcgg ctggaaataa tcatcattaa acagacgacc accaccaagg
     6541 caggaccacg ccatcggacg aacacgcagt tgttgtagtt ggtcgagcgt gccatccagc
     6601 agtaacggct gatgcaccgg ggatatttcc acctgattag tggcaagggt aaacggcaga
     6661 cgtgattgca acagggcaaa ttgcgcaggc gtaaagttcg atacgccaaa atgacgcact
     6721 ttgccgctct gatgcagatg tttgaacgcg tccgccactt catcggcatc cattaacggg
     6781 tctggtcggt ggattaacag cagatccaga tgatcggtcg cgagattaat tagcgactgt
     6841 tcggcgctct taatgatgtg attgcggtca gtgatgtaat gaccaatgac gttttcttca
     6901 cgcgcggtcg tcgcgatacc gcatttactg acgatttcca tccgttcacg caggtgaggt
     6961 gccagtttca gtgcctcgcc aaacgccgct tcgcactgat agccaccata aatatcagca
     7021 tggtccacgg tggtcacgcc gagatccaga tgctcttcaa taaaactgac cagctggcgg
     7081 gcggacatat tccagtccat caatcgccag tagcccatca caaaacggga aaactccggg
     7141 ccttccggcg caatagtaat acgctgaacc ataatcgctt cctcttatca gatataagag
     7201 gagtatacgc aagattaggt tcaaaagagt gatggttgct ccggttcgtc tgatgacgct
     7261 ggcttatttg cgcgtaattt gcgcattaat cgctgccgac aaaggcgcag cacctcttgt
     7321 ttttcgccat cgctcatttt attccagtta aaacgctcat cccgactacg aaaacagccg
     7381 cgacaaaacc cgcgttcgtc agactggcaa attccccggc acgggctctg gacgggaaag
     7441 aactctaatt gctccgccac ctcgccctcc tcagataaga ttattaccat tattgaagct
     7501 gttaatgtcc aaagtagcaa ctttgcttgc actagaccaa ctggtctact acactccaac
     7561 gcatgaacaa acacaccgaa catgatactc gcgaacatct cttggcgacg ggcgagcaac
     7621 tttgcctgca acgtggattc accgggatgg ggctaagcga attactaaaa accgctgaag
     7681 tgccgaaagg gtccttctat cactactttc gctctaaaga agcgtttggc gttgccatgc
     7741 ttgagcgtca ttacgccgca tatcaccagc gactggctga gttgctgcaa tccggcgaag
     7801 gtaactaccg cgaccgcata ctggcttatt accagcaaac actgaaccag ttttgccaac
     7861 atggaaccat cagtggttgc ctgacagtaa aactctctgc cgaagtgtgc gatctgtcag
     7921 aagatatgcg cagcgcgatg gataaaggtg ctcgcggcgt gatcgccctg ctctcgcagg
     7981 ctctggaaaa tggccgtgag aaccattgtt taaccttttg tggcgaaccg ctacaacagg
     8041 cacaagtgct ttacgcacta tggcttggcg cgaatctgca ggccaaaatt tcgcgcagtt
     8101 tcgagccact ggaaaacgcg ctggcccatg taaaaaacat tattgcgacg cctgccgttt
     8161 agcaggcatt ttttatcacc agacgaccgg gagcctttat gtcatctgaa aaactgtatt
     8221 ccccactgaa agtgggcgcg atcacggcgg caaaccgtat ttttatggca ccgctgacgc
     8281 gtctgcgcag tattgaaccg ggtgacattc ctaccccgtt gatggcggaa tactatcgcc
     8341 aacgtgccag tgccggtttg attattagtg aagccacgca aatttctgcc caggcaaaag
     8401 gatatgcagg tgcgcctggc atccatagtc cggagcaaat tgccgcatgg aaaaaaatca
     8461 ccgctggcgt tcatgctgaa aatggtcata tggccttgca gctgtggcac accggacgca
     8521 tttctcacgc cagcctgcaa cctggcggtc aggcaccggt agcgccttca gcacttagcg
     8581 cgggaacacg tacttctctg cgcgatgaaa atggtcaggc gatccgcgtt gaaacatcca
     8641 tgccgcgtgc gcttgaactg gaagagattc cgggtatcgt caatgatttc cgtcaggcca
     8701 ttgctaacgc gcgtgaagcc gcttttgatc tggtagagct ccactctgct cacggttatt
     8761 tgctgcatca gttcctttcc ccttcttcaa accatcgtac cgatcagtac ggcggcagcg
     8821 tggaaaatcg cgcacgtttg gtactggaag tggtcgatgc cgggattgaa gaatggggtg
     8881 ccgatcgcat tggcattcgc gtttcgccaa tcggtacttt ccagaacaca gataacggcc
     8941 cgaatgaaga agccgatgca ctgtatctga ttgaacaact gggtaaacgc ggcattgctt
     9001 atctgcatat gtcagaacca gattgggcgg ggggtgaacc gtatactgat gcgttccgcg
     9061 aaaaagtacg cgcccgtttc cacggtccga ttatcggcgc aggtgcatac acagtagaaa
     9121 aagctgaaac gctgatcggc aaagggttaa ttgatgcggt ggcatttggt cgtgactgga
     9181 ttgcgaaccc ggatctggtc gcccgcttgc agctcaaagc tgagcttaac ccacagcgtg
     9241 ccgaaagttt ctacggtggc ggcgcggaag gctataccga ttacccgacg ttgtaatcca
     9301 acattgcgag cggcgtaaag ccgccgctat actaaaacaa cattttgaat ctgttagcca
     9361 ttttgaggat aaaaagatgc gtcttcttca taccatgctg cgcgttggcg atttgcaacg
     9421 ctccatcgat ttttatacca aagtgctggg catgaaactg ctgcgtacca gcgaaaaccc
     9481 ggaatacaaa tactcactgg cgtttgttgg ctacggcccg gaaaccgaag aagcggtgat
     9541 tgaactgacc tacaactggg gcgtggataa atacgaactc ggcactgctt atggtcacat
     9601 cgcgcttagc gtagataacg ccgctgaagc gtgcgaaaaa atccgtcaaa acgggggtaa
     9661 cgtgacccgt gaagcgggtc cggtaaaagg cggtactacg gttatcgcgt ttgtggaaga
     9721 tccggacggt tacaaaattg agttaatcga agagaaagac gccggtcgcg gtctaggcaa
     9781 ctaatctcct gccgggcgtg aactcatcgc gcccgcatct ttactgcatc gacaagtaat
     9841 atttgtcata atgcgcgctg caatttatcc gtattaagag aatcagatgt ccgataacgc
     9901 tcaacttacc ggtctgtgcg accgttttcg tggtttttat cctgttgtga tcgatgttga
     9961 aacagccgga tttaacgcca aaaccgatgc gctgcttgag attgccgcca tcaccctgaa
    10021 aatggatgaa caaggctggc tgatgccgga caccacatta catttccacg tcgaaccatt
    10081 tgtcggcgca aatttgcaac cagaagccct cgccttcaac ggcattgacc cgaacgatcc
    10141 cgatcgcggc gcggtcagcg aatacgaggc gctgcacgaa atttttaaag ttgtacgtaa
    10201 aggtattaaa gcgagcggct gtaaccgcgc cattatggtg gcgcacaacg ccaattttga
    10261 tcacagcttt atgatggccg ccgcagaacg cgcctcgctg aaacgtaacc cgttccaccc
    10321 tttcgccact tttgacactg ctgcactggc cgggctggca ctcggacaaa ccgtattgtc
    10381 aaaggcttgc cagaccgctg gcatggactt cgacagcacc caggcgcact ccgcgctgta
    10441 cgacaccgaa cgcactgctg tgctgttttg tgaaatcgtc aaccgctgga aacgtctggg
    10501 aggctggccg ctacctgccg ccgaagaggt gtaatcgagt cgatgcctga tgacatgcaa
    10561 tgattcaggc atctatagtg aggctattcc acgcatcaag catgatattc acggggaata
    10621 gcgttaatgg cagataatcc agacccttca tcgctcctgc cggacgtgtt ttcaccggcg
    10681 acccgcgact ggtttcttcg cgcctttaaa cagccgaccg ctgtccagtc gcaaacctgg
    10741 catgtggcgg cgcgaggcga acatgcgctg gtgattgcac cgactggctc cgggaaaacg
    10801 ctggcagcat ttctctacgc cctcgatcgg ctcttccgcg aaggcggcga agatacccgc
    10861 gaggcgcata agcgtaaaac ctcacgcatc ctctatattt caccgataaa agccctgggc
    10921 accgacgttc agcgcaactt gcagatcccg ttgaagggta ttgccgatga acggcggcgg
    10981 cgcggcgaaa cggtaattat tgatgaagtc cacgcggtag cgggcagtaa acgtggtgcg
    11041 catctggcgt taagtctgga gcggctcgat gcgctgctcc acacctcagc acagcgaatt
    11101 ggcctttctg ccactgtgcg ctcagccagc gatgtggcag catttcttgg tggcgatcgc
    11161 ccggttacgg tagtcaaccc gcccacaatg cgccatccgc agatacgaat tgtcgtaccg
    11221 gtcgccaata tggatgatgt ctcatcggtc gccagcggca ccggcgaaga cagccatgcc
    11281 ggccgggaag gctccatctg gccatatatt gaaacgggta tccttgatga agtgttgcgc
    11341 catcgctcga ccattgtctt tactaattca cgcgggctgg cggaaaaact gacggcacga
    11401 ttaaatgagc tttacgccgc acgcttacag cgttccccgt ctatcgccgt tgatgcggcc
    11461 catttcgagt cgacctccgg cgcaacctct aaccgtgtac aaagtagcga cgtttttatt
    11521 gcccgctcac accacggctc cgtctctaaa gaacaacgag caatcaccga acaggcgctg
    11581 aaatcgggtg aattacgctg cgtggtcgca acctccagtc ttgaactggg gattgatatg
    11641 ggcgcggtgg atctggtgat tcaggtggca acgccgcttt ctgttgccag tgggttacaa
    11701 cgcattggtc gcgccggaca tcaggttggc ggtgtatcta aagggctgtt tttcccccgt
    11761 acccggcgtg atttagtcga ttccgcagtc attgtagagt gtatgttcgc aggcaggctg
    11821 gaaaacctga caccaccgca taatcctctc gacgtccttg cgcagcaaac cgttgccgcc
    11881 gcggcgatgg atgcattaca ggtagacgaa tggtactccc gcgtacgccg taccgcaccg
    11941 tggaaagatc tgccaagacg tgtttttgac gccacgctgg atatgctttc cgggcgctat
    12001 ccctctggcg atttttctgc ttttcgcccc aaactggtct ggaacaggga gaccgggata
    12061 ttgaccgccc gacctggcgc tcaattgttg gcggttacca gcggcggcac cattccggat
    12121 cgtggcatgt atagcgtgtt attacccgaa ggtgaagaaa aggccggttc gcggcgggtg
    12181 ggtgaactgg atgaggagat ggtatatgag tcgcgggtga acgacattat cactctcggc
    12241 gctacctcat ggcggatcca gcaaatcacc cgcgatcagg tgattgtgac tcctgctccg
    12301 ggtcgttctg cccagctccc cttctggcgt ggtgaaggta acggacgtcc ggctgaatta
    12361 ggcgagatga tcggcgattt tcttcattta ctggcggatg gcgcgttctt ttccgggact
    12421 attcccccgt ggctggcaga agaaaatacg aacgccaata ttcaggggtt gattgacgag
    12481 caacgcaacg cgacgggcat cgttccgggg agtcgccatc tggtcctcga acggtgccgt
    12541 gatgaaattg gcgactggcg tattattttg cactctccct atggaagacg ggtgcatgaa
    12601 ccctgggcgc tggcgattgc cgggcgaata catgccctat ggggcgctga cgcgtctgtg
    12661 gtcgccagtg atgacagcat tgttgcgcgc attcctgaca ctgatggtaa attgcccgac
    12721 gccgcgattt ttttgtttta accagaaaag ttgctgcaaa ttgtccgcga ggcggtaggc
    12781 agctcggcac ttttcgccgc ccgttttcgc gaatgcgccg cgcgggcatt attaatgccg
    12841 ggccgcacgc cgggccatcg caccccgctt tggcaacaac ggctgcgcgc cagtcagttg
    12901 ctggaaatcg ctcagggata tccggatttt ccggtcattc tcgaaaccct acgcgaatgt
    12961 ctgcaagatg tttatgatct tcccgcactg gaacgtttga tgcgtcgcct gaacggtggc
    13021 gaaattcaaa tatccgatgt aacgaccact acgccctcgc ctttcgccac aagtttattg
    13081 ttcggctatg tcgcggaatt tatgtaccag agcgacgccc cgctggcaga gcgccgggca
    13141 tccgtactgt cgctggacag cgagttactg cgcaatctac tcggacaggt cgatccgggg
    13201 gaattactcg acccgcaggt cattcgccag gtggaagaag agttgcaacg actggctcct
    13261 ggtagaagag cgaaaggtga agaaggattg ttcgacctgc tgcgcgaact ggggccaatg
    13321 accgttgaag acctggcgca acggcataca ggcagcagtg aagaggttgc gtcgtatctg
    13381 gaaaatcttc ttgcagtaaa acgaatcttc ccagcgatga ttagcggaca ggagcgtctt
    13441 gcctgtatgg atgatgccgc caggctgcgt gatgccctcg gcgtacggct accagagtca
    13501 ttgccagaga tttatttaca tagagtcagt tacccgcttc gcgacctctt tctgcgctat
    13561 ctccgggctc atgctctggt cacggctgaa caactggctc atgagtttag tctcggtatt
    13621 gccattgtcg aagagcagct tcagcaactg cgtgaacagg gtctggtgat gaatctgcaa
    13681 caagacatct gggtgagcga tgaagtattt cgtcgtctgc gtttgcgctc gctgcaagcc
    13741 gccagagaag cgacgcgtcc cgttgcagcc acgacctatg cgcgattgct gctggaacgt
    13801 cagggcgtat tacccgccac cgatggtagc ccggcgctct ttgcctcaac atctccaggc
    13861 gtttatgagg gcgtagatgg agtgatgcgg gtgatcgaac agcttgccgg agtcggttta
    13921 cccgcctcac tctgggaaag ccagatcctg cctgcccgcg tacgcgacta ttcgccagaa
    13981 atgctcgatg aattactggc aaccggagcg gttatctggt cgggacaaaa aaaagctggg
    14041 tgaagatgac ggcctggtgg cactgcatct acagaaatat gccgcagaat cgttcactcc
    14101 cgccgaagcg gatcaggcga atcgttcggc gctgcagcaa gcgataatcg ctgttctggc
    14161 tgacggagga gcctggtttg cacaacaaat cagccaacgg atacgcgata aaatcgtcga
    14221 atcggttgat tcctcggcac tgcaagaggc gttatgggcg ctggtctggc aaggcgtcat
    14281 taccagcgat atttgggcac cgttacgcgc cctcacccgc agcagttcca acgcacgcac
    14341 ctcaactcgc cgcagtcacc gggctcgtcg tgggcgtcct gtctatgcac aacccgtctc
    14401 gccgcgggta tcttacaaca caccaaatct ggctggacgc tggtcgttat tgcaggtgga
    14461 gccactaaac gataccgaaa ggatgctggc gctggcggaa aatatgctcg accgctacgg
    14521 catcatcagt cgtcaggcgg tgatagccga aaatatccct ggcgggtttc catcgatgca
    14581 aacactttgt cgaagtatgg aagactccgg gcgaattatg cgaggtcgtt ttgtagaagg
    14641 tctgggtggc gcgcaattcg ctgaacgtct gactattgac cgattgcgcg atctggcgac
    14701 acaagccacg caaacacgcc actatacacc agtggcgctc tccgccaacg atccggctaa
    14761 tgtgtgggga aatcttctgc cctgggctgc acatccggca acgctggtac caacgcgtcg
    14821 ggcgggtgcg ctggtggtcg tttctggcgg caaattgtta ctctatctgg cgcaaggcgg
    14881 caaaaaaatg ctggtctggc aggaaaagga ggaattactc gccccagaga ttttccaggc
    14941 gctgaccacc gcactgcgtc gcgaaccaag gctgcgcttt acgctaacag aagtgaatga
    15001 tcttccggtc cggcaaacgc cgatgtttac gctgctgcgt gaggcgggat tttcaagttc
    15061 gccacaaggg ctggattggg gatagttgac atcctccacg ccctgaaaga cgtggttccc
    15121 tgctacgttc aagctgtcgc ctgaatcatt tcggtgggtt cctgcttcaa cgagcggcct
    15181 gactgcacca tctctccaca ggcaagaacg gcgtgccccg ccgctaaaat gttacgagcg
    15241 ccatttacat cggcgttcgc tgtatatcca catacctggc atctgaattt actttgtgac
    15301 aggcggtttt ctttcgctgt atgaccacag cacgcgcaac gctggcttgt gtacgctggc
    15361 ggaacagcaa gcacctgacc gccactccag agctgcttgt actcaagctg gcggcgcatt
    15421 tcataccagc cctgatccag tatcgaacgg tttaaacctg attttgcccg gacattgcgc
    15481 cccggctggc tgaccgtacc cgctgctgac tttgacatgt tgctgacctt caaatcctca
    15541 atgactatca ttgcgtggtt tttgctgacg gtcgttgtga ctttgtgaag gtagtccctg
    15601 cggatatttg cgatacagga atgcagtcgc tgtattttgc gtttctgctt ctgccagttg
    15661 ttgctgaatt tgaccttccg gcttaactgt cgctgaagtc tcgccagcgt cttctggttt
    15721 ttttgaaaac tgtttacagg ctcaaagact gtgccatctg acagcgtggc gagtttagcc
    15781 acgccagcat ccagcccgat cattgatgct gaagggtgaa ccggagttga tacttcactt
    15841 tctgtctgaa tactgatgta ccacgtaccg caggactggc tgacagtgac atttttcaca
    15901 actcccgtga cctgacggct attccggtag cgcatccagc caagtttcgg cagaaaaata
    15961 cggctgtttt cctgatcgag cttaacgtcc tgcgggtagc ggaatgcagc attctgtccc
    16021 cgctttttga atcggggaaa agcagcccgc ttctggaaga agtttttgta agcccgctca
    16081 aggtctttca gtgactgttg caatggctgt gagggcgaat ctttaagcca ttgcgtttca
    16141 gtggcgtttt tccactcaac cagccaggaa gccattttcc cgtaagggat gtatttatta
    16201 ccggcctcat gattctcatt ctgaagcgcc agagcacgat tgaaaacgaa acgacatgcg
    16261 cctgcgaagc gcctcatttc acgctcctgt tgaccaccgg gtcttaactg gaatttaaat
    16321 gcctgtaatc gtttcacagg tcaaatgata ctttggtctg tgaaaaaaga aaccgatatc
    16381 cggcgtggaa ggcattgtgt tttcctgatg catgttcacc tggtctttgt caccaaatac
    16441 cgacgccaga tttttgacca tgacacgaca gaaaaactac gcacttactt ttcaaatgta
    16501 tgtgctgatt ttgcagctga actggttgaa atggatggcg aaccagatca cgtccatttg
    16561 ttaattaact atcctcccaa actggcgata tccagtctgg taaacagcct caaaggcata
    16621 tcgaataggt tactgcgacg agatcgacca gatattgcag tcaggtatta ctacaaaggc
    16681 gttttgtgga gtcctggcta ttttgccagt agctgcgaag gtgcgccaat atccgtcatc
    16741 cgccagtaca ttgaacaaca gcaaacacct ggtcaggtgg aaaaccgcgc cttatatccc
    16801 cgccctgaag gacgaggttt tacggcgcac cggataaagg actgacggat gcccgttcgc
    16861 atccgtcagt attgcaggac ggattattcc gcgtccggct cttcagactt gtatttagcg
    16921 gcagtttctt tgatcagctg ctgcagttcg ccacgctgat acatttcgat cacgatatca
    16981 caaccgccga ccagctcgcc gtcaacccac agttgcggga aggtcggcca gttagcatat
    17041 ttcggcagtt ccgcacgaat gtccggattc tgcagaatat caacataggc aaaacgttcg
    17101 ccacatgcgg caagcgcctg gactgcctgg gcagagaaac cgcagctcgg cagtttcggt
    17161 gaacctttca tgtacagcag gatcgggttt tcagcaatct ggcgttggat tttttcgata
    17221 gtggtgctca ttgtcttgct tcctcaaact tctttacggc agtaatctga cattgtagcg
    17281 ggtcagtgcg gcatcggaaa ataacatttt catcacgctt ttgctatttt atccctttgc
    17341 tctatttgtt gcatttcaaa tattcgtttc ttctctgatg cgcggcaaac tggcgtattt
    17401 ataaccattt ttagctttca ctgctgcatt tttttgcgct cgccaacgaa acgtattttt
    17461 taacaataaa agctattaac tttctcttct tctatgcatt agaatcatca agttttgtaa
    17521 atcagacgca ggcatgatag acctgcctta acagagggac gctcagtggc gcggataaac
    17581 cgtatttcga tcacgctctg tgctttactt tttaccaccc tgcctttaac gcctatggcc
    17641 catgcttcaa agcaagccag ggagagttct gctaccactc atatcaccaa aaaagcagat
    17701 aaaaagaaaa gcacggcaac caccaaaaag acccagaaag ccagccagaa aacagcgaaa
    17761 aaagccgcca gtaaaagtat gaccaaaagc aaaaccgctt cttctgttaa aaaatcttcc
    17821 attaccgctt ctaaaaacgc caaaactcgc agcaaacacg ccgtcaataa aacggcctca
    17881 gccagcttta ccgaaaagtg taccaaacgt aagggctata aatcgcattg tgtgaaagtc
    17941 aaaaatgccg cgtcaggaac ccttgccgac gcgcacaaag cgaaggtgca aaaagccacg
    18001 aaagtggcaa tgaataaact gatgcagcaa attggtaagc catatcgttg gggcggcagc
    18061 tcaccgcgta ccggttttga ttgcagcggc ctggtttatt acgcttataa agatttggtg
    18121 aaaattcgta ttccgcgtac ggcgaatgaa atgtatcacc tgcgtgatgc aggacctatc
    18181 gaacgtagtg agctgaaaaa cggcgacctg gtctttttcc gtactcaggg acgcggtaca
    18241 gccgatcatg tcggcgtgta tgtcggcaac ggcaaattta ttcagtcacc gcgcacaggt
    18301 caggaaattc aaatcacttc tctcagtgaa gactactggc agcgccacta tgttggcgct
    18361 cgtcgggtaa tgaccccaaa aacacttcgc taaaacttta ccctgttgtt acggcaacag
    18421 ggtaagttca tcttttgtct caccttttaa tttgctaccc tatccatacg cacaataagg
    18481 ctattgtacg tatgcaaatt aataataaag gagagtagca atgtcattcg aattacctgc
    18541 actaccatat gctaaagatg ctctggcacc gcacatttct gcggaaacta tcgagtatca
    18601 ctacggcaag catcatcaga cttatgtcac taacctgaac aacctgatta aaggtaccgc
    18661 gtttgaaggt aaatcactgg aagagattat tcgcagctct gaaggtggcg tattcaacaa
    18721 cgcagctcag gtctggaacc atactttcta ctggaactgc ctggcaccga acgccggtgg
    18781 cgaaccgact ggaaaagtcg ctgaagctat cgccgcatct tttggcagct ttgccgattt
    18841 caaagcgcag tttactgatg cagcgatcaa aaactttggt tctggctgga cctggctggt
    18901 gaaaaacagc gatggcaaac tggctatcgt ttcaacctct aacgcgggta ctccgctgac
    18961 caccgatgcg actccgctgc tgaccgttga tgtctgggaa cacgcttatt acatcgacta
    19021 tcgcaatgca cgtcctggct atctggagca cttctgggcg ctggtgaact gggaattcgt
    19081 agcgaaaaat ctcgctgcat aataactgat ggcaaatgca gcattgcctg atgcgcttcg
    19141 cttaccatgc caacgcggat gaggcgttaa cagcgcattc ggcaagataa atcgcacgtt
    19201 gtcagcaact gtaacgcaga aggttatcct tctgcgtttt tgtttaatta gctgttagca
    19261 acgcaaactg tttcaggttg ttttctggct gacataaaca ccagcaataa tgccagtccc
    19321 gcgacaatcg cccccgtcac cggcacaaag ctgtatccca gcccagcgga aattaccgca
    19381 ccaccagcag ctgctcccag cgcatttcca agattaaagg caccaatatt gactgatgaa
    19441 gacagacctg gcgcttcact ggcgacatgc atcacgcgca tctgtaacgg aggtacaacc
    19501 gcaaaggttg ctgcgcccca caccaccatg ctaatagctg cgccgaactc attgcgggcc
    19561 aggaacggga ttgccagcat aatcaccatc agcagcaaca aaaagccttt caacgtgccg
    19621 ttaactgaac gatctgccag tttgccgccg agatagttac cgatagagaa tccgacacca
    19681 atcagcacca gcattgccgt gacgaacacc ggtgttgcgt gggtaatact ttgcagtacc
    19741 ggagagatat aggtgtagag ggtaaacatt gcaccagctc ccagtaccgt cgtcagcaat
    19801 gcagacagca cctgcggacg cattaatacc gccagctctt ttttcacttc aggtcgtgcc
    19861 cctgtaccac ctttaggtaa tgagaagaac agacttacca ttgaaatcac tcccagcccc
    19921 gccgttgcca gaaatgacat ccgccagccg atggtttcac ccaaccaggt cgccgccggc
    19981 acgccaccga tatttgccag ggttaacccc ataaacatag tggcaactgc gctggcctgt
    20041 ttatgttttg gcaccacgct tgcggccacg actgaaccca aaccaaaaaa tgctccgtga
    20101 ttcaggctgg tcaaaatacg tgaaagcatc agggtcatat aatccggcgc gatggcggaa
    20161 agaacgttgc ctagcgtgaa aattgccatc aggaaaatca acgcactgcg gcgggcacga
    20221 tgagaaagta gaagcgtcat cagcggcgcg cccaccatta cgccaactgc ataggcactg
    20281 attaacattc cggcagcggg aatcgagaca tccacaccgc gcgcaatgac gggcaacaag
    20341 cccattggcg agaactccgt tgtcccgata ccaaacgcgc caatcgccag cgccagcaac
    20401 ggatagttaa ttttcatgcc ttatctccac ctcttcgcgt cattacgcga tattcattaa
    20461 agtggcgaaa gcatgacagc aatcacaaaa aaatgaaaat aacaaaaaga gaaaacactt
    20521 ttgccatttt gctaacaaac aggaaggaga tgcgagggag aacgcgctcc ctcgagagga
    20581 aatcagtgca gcgcggcagt caaacccacg gctacgatca aaccgaggac gataaccgtt
    20641 gttaccagtg aaaatttaag gtcggtgctc atcaagtttt ctcctttttt attaccacac
    20701 aaaaagtgat attacgcatt tttacacact gtgatgaaaa aatctcccgt catttataat
    20761 gataagtgtt tttaccactt ccccttttcg tcaagatcgg ccaaaattcc acgcttacac
    20821 tatttgcgta ctggccattg accccttcct gacgctccgt gtcgtttttc cggcgtaccg
    20881 caacactttt gttgtgcgta aggtgtgtaa aggcaaacgt ttaccttgcg attttgcagg
    20941 agctgaagtt agggtctgga gtgaaatgga atggcaacaa taaaagatgt agcgaaacga
    21001 gcaaacgttt ccactacaac tgtgtcacac gtgatcaaca aaacacgttt cgtcgctgaa
    21061 gaaacgcgca acgccgtgtg ggcagcgatt aaagaattac actactcccc tagcgcggtg
    21121 gcgcgtagcc tgaaggttaa ccacaccaag tctatcggtt tgctggcgac cagcagcgaa
    21181 gcggcctatt ttgccgagat cattgaagca gttgaaaaaa attgcttcca gaaaggttac
    21241 accctgattc tgggtaatgc gtggaacaat cttgagaaac agcgggctta tctgtcgatg
    21301 atggcgcaaa aacgcgtcga tggtctgctg gtgatgtgtt ctgagtaccc agagccgttg
    21361 ctggcgatgc tggaagagta tcgccatatc ccaatggtgg tgatggactg gggtgaagca
    21421 aaagctgact tcaccgatgc ggtcattgat aacgcgttcg aaggcggcta catggccggg
    21481 cgttatctga ttgaacgcgg tcaccgcgaa atcggcgtca tccccggccc gctggaacgt
    21541 aacaccggcg caggccgcct tgccggtttt atgaaggcga tggaagaagc gatgatcaag
    21601 gtgccggaaa gctggattgt gcagggtgac tttgaacctg aatccggtta tcgcgccatg
    21661 cagcaaatcc tgtcgcagcc gcatcgccct actgccgtct tctgtggtgg cgatatcatg
    21721 gcaatgggcg cactttgtgc tgctgatgaa atgggcctgc gcgtcccgca ggatgtttcg
    21781 ctgatcggtt atgataacgt gcgcaacgcg cgctatttta cgccggcgct gaccacgatc
    21841 catcagccaa aagattcgct gggtgaaaca gcgttcaaca tgctgttgga tcgtatcgtc
    21901 aacaaacgtg aagaaccgca gtccattgaa gtgcatccgc gtttgattga acgccgctcc
    21961 gtggctgacg gcccgttccg cgactatcgt cgttaatcac ccgttgcggg agtctcttcc
    22021 ggctcccgca gccactcctt attcagcgtc tcactatcgc tgagatactc aagcagccag
    22081 gttaacgcag gcgacatatc attttgctgc catgtcagac aacatgccga atccgggaag
    22141 gggttttcca gttcaagtgc tacccacttc ccctcattaa gccacggttt ggcgaaatgt
    22201 gttggcacca tccctatgca taatcctgcc gagatacagg ttgccgatga ttcccagtca
    22261 ggtacgacaa gtcttttttg gttatccagc aaccaggtaa tacgtttagg tagcgttcgc
    22321 gaggtgtctt cgcgcaccaa cgacggccag ttgcgcaacg tatcatcgct gaacgggcca
    22381 tccatcaacg ccagcgggtg gtgactggca acaacgcaac tccagcttag catccccata
    22441 tcccggaagg cataacgacc gcctaccgga atcgcgcgtg ttgcgccaat tgccagttcc
    22501 acgcgcccgt cggaaagcgc atcccagaca ccgttgaaca cttcctgaaa gacaagaagt
    22561 tcgacatcat caaaatggcg ataaaaatca acgatcatct gccgtgtacg ttctggcctg
    22621 acaatattat ccactgcgat agctaactga ccgcgccagc cgttcgctat ctgctgacat
    22681 tgctggcggg tgatctgcat ttttttgaca acagagcgcc cttctttgag aaaccacgct
    22741 ccagcagcgg tcagctccac atcacggtgc cgtcgttcaa agagcggtac cgccagccac
    22801 tcttccagct gacgcacggt atagctgacc gcagaaggaa cacgatgcag ctcctgtgcc
    22861 gcagcgctaa aactaccatt acgcgctacc gcatcaacaa cttcgagtga atattctgac
    22921 cacatagtct gcctgcaaaa tttttgaaac cagtcatcaa atattaccgt ttcacaacac
    22981 taatttcact ccctacactt tgcggcggtg tttaattgag agatttagag aatatacatg
    23041 caacctggga aaagattttt agtctggctg gcgggtttga gcgtactcgg ttttctggca
    23101 accgatatgt atctgcctgc tttcgccgcc atacaggccg acctgcaaac gcctgcgtct
    23161 gctgtcagtg ccagccttag tctgttcctt gccggttttg ccgcagccca gcttctgtgg
    23221 gggccactct ccgaccgtta tggtcgtaaa ccggtgttat taatcggcct gacaattttt
    23281 gcgttaggta gtctggggat gctgtgggta gaaaacgccg ccacgctgct ggtattgcgt
    23341 tttgtacagg ctgtgggggt ctgcgccgcg gcggttatct ggcaagcatt agtgacagat
    23401 tattatcctt cacagaaagt caaccgtatt tttgcgacca tcatgccgct ggtgggtctg
    23461 tctccggcac tggctcccct gttaggaagc tggctgctgg tccatttttc ctggcaggcg
    23521 attttcgcca ccctgtttgc cattaccgtg gtgctgattc tgcctatttt ctggctcaaa
    23581 cccacgacga aggcccgtaa caatagtcag gatggtctga cctttaccga cctgctacgt
    23641 tctaaaacct atcgcggcaa cgtgctgata tacgcagcct gttcagccag tttttttgca
    23701 tggctgaccg gttcaccgtt catccttagt gaaatgggct acagcccggc agttattggt
    23761 ttaagttatg tcccgcaaac tatcgcgttt ctgattggtg gttatggctg tcgcgccgca
    23821 ctgcagaaat ggcaaggcaa gcagttatta ccgtggttgc tggtgctgtt tgctgtcagc
    23881 gtcattgcga cctgggctgc gggcttcatt agccatgtgt cgctggtcga aatcctgatc
    23941 ccattctgtg tgatggcgat tgccaatggc gcgatctacc ctattgttgt cgcccaggcg
    24001 ctgcgtccct tcccacatgc aaccggtcgc gccgcagcgt tgcagaacac tctgcaactg
    24061 ggtctgtgct tcctcgcaag tctggtagtt tcctggctta ttagtatcag cacgccattg
    24121 ctcaccacca ccagcgtgat gttatcaaca gtagtgctgg tcgcgctggg ttacatgatg
    24181 caacgttgtg aagaagttga ctgcccgaat catggcaatg ccgaagtcgc tcatagcgaa
    24241 tcacactgat ctatatcgat atacttatac ttaggctgct aacaaaattt tgttgtatga
    24301 ttgaaattag cggcctatac taatttcgag ttgttaaagc tacgataaat attatgtttt
    24361 tacggggaca ggatcgttcc cgactcacta tggatggtca tttcggcaag ggttcctcct
    24421 ttccctctgt tctacgtcgg attatagact cgcggttttt tctgcgagat ttctcacaaa
    24481 gcccaaaaag cgtctacgct gttttaaggt tctgatcacc gaccagtgat ggagaaacta
    24541 tgagttcatc gtgtatagaa gaagtcagtg taccggatga caactggtac cgtatcgcca
    24601 acgaattact tagccgtgcc ggtatagcca ttaacggttc tgccccggcg gatattcgtg
    24661 tgaaaaaccc cgattttttt aaacgcgttc tgcaggaagg ctctttgggg ttaggcgaaa
    24721 gttatatgga tggctggtgg gaatgtgacc gactggatat gttttttagc aaagtcttac
    24781 gcgcaggtct cgagaaccaa ctcccccatc atttcaaaga cacgctgcgt attgccggcg
    24841 ctcgtctctt caatctgcag agtaaaaaac gtgcctggat agtcggcaag gagcattacg
    24901 atttgggtaa tgacttgttc agccgcatgc ttgatccctt catgcaatat tcctgcgctt
    24961 actggaaaga tgccgataat ctggaatctg cccagcaggc gaagctcaaa atgatttgtg
    25021 aaaaattgca gttaaaacca gggatgcgcg tactggatat tggctgcggc tggggcggac
    25081 tagcgcacta catggcatct aactatgacg taagcgtggt cggcgtcacc atttctgccg
    25141 aacagcaaaa aatggctcag gaacgctgta aaggcctgga tgtcaccatt ttgctgcaag
    25201 attatcgtga cctgaacgac cagtttgatc gtattgtttc tgtggggatg ttcgagcacg
    25261 tcggaccgaa aaattacgat acctattttg cggtggtgga tcgtaatttg aaaccggaag
    25321 gtatattcct gctccatact atcggttcga aaaaaaccga tctgaatgtt gatccctgga
    25381 ttaataaata tatttttccg aacggttgcc tgccctctgt acgccagatt gctcaatcca
    25441 gcgaacccca ctttgtgatg gaagactggc ataacttcgg tgctgattac gatactacgt
    25501 tgatggcgtg gtatgaacga ttcctcgccg catggccaga aattgcggat aactacagtg
    25561 aacgctttaa acggatgttt acctattatc tgaatgcctg cgcaggtgct ttccgcgccc
    25621 gtgatattca gctctggcag gtggtgttct cacgcggtgt tgaaaacggc cttcgagtcg
    25681 ctcgctaaag gctattctat cgccccctct tcgggggcga gttcagatca ggcttctgta
    25741 cctggttgat tcatggcatt ttctcgtgcc gccagcacac gttctaccgt atctaccact
    25801 gcctgagttt gtggatcgat ttcaatgttg acgcgtgcgc caagtttttt cttcccaaga
    25861 gtcgtgcgtt ccagtgtttc cggaattaaa tggacgcaaa aacgcgttgg cgtgacttcg
    25921 ccgacggtca ggctaatacc gtcgatgcca ataaatcctt tgtacaggat atatttcatc
    25981 aactgactat cctggacttt aaaccagatc tggcgattat tttccgaggt taatattttc
    26041 gccacttcag cggtggtcat aatatgacct gacattaagt gtccgccaat ttcatcactg
    26101 aatttcgccg cacgctcaac gtttacccaa tcccccactt ttaaatcgcc aagattggta
    26161 atgcgtaacg tttctttcat caggtcaaaa ctgacatggt tgccgttaat ttccgtcacg
    26221 gtcaggcagc aaccgttatg cgccacggaa gcaccggttt ccaggccgtc cagcatgtgg
    26281 tcgggtaact ccaccacatg cgtacgaaaa tttggtttct cgtcaatcga caccagtttt
    26341 gcggtgccct gtacaatccc cgtaaacata cttacaactc ctgaaatcag ttaagacatt
    26401 ctgttcagca caatagcagg tggaaaacgc ccttaccagt gaaggggtaa gaatggctat
    26461 tttttcactg gagaattaat aaatcctcgc tacaatagac tgaatttccc ctgcttcttc
    26521 tttttgctgc ccattcaggc ggctttttag tctctcatat aactacaaat aaaaggtgtt
    26581 cacgtgcaga agtatatcag tgaagcgcgt ctgttattag cattagcaat cccggtgatt
    26641 ctcgcgcaaa tcgcccaaac tgcgatgggt tttgtcgata ccgtgatggc gggcggctat
    26701 agtgccaccg acatggcggc ggtcgctatc ggtacttcta tctggcttcc ggcgatcctc
    26761 tttggtcacg gactgctgct ggcattaacg ccggttatcg cgcaattaaa tggttccggt
    26821 cgacgtgagc gcattgcgca tcaggtgcga caaggtttct ggctggcagg ttttgtttcc
    26881 gttctcatta tgctggtgct gtggaatgca ggttacatta tccgctacat ggaaaacatc
    26941 gatccggctc tggcggacaa agccgtgggt tatctgcgtg cgttgttgtg gggcgcgccg
    27001 ggatatctgt tcttccaggt tgcccgtaac caatgtgaag gtctggcaaa aaccaagccg
    27061 ggtatggtaa tgggctttat cggcctgctg gtgaacatcc cggtgaacta tatctttatt
    27121 tatggtcatt tcggtatgcc tgagctcggt ggcgttggtt gtggcgtggc tactgcggcg
    27181 gtgtattggg tcatgttcct tgccatggtt tcttacatta aacgcgcccg ctccatgcgc
    27241 gatattcgta acgaaaaagg caccgctaag cccgagcctg cggttatgaa acgactgatt
    27301 caactcggtt tgccgattgc gctggcactg ttctttgaag tgacactgtt tgccgtcgtg
    27361 gctctgttag tgtctccgct cggtattgtt gatgtcgcag gacaccagat tgccctgaac
    27421 tttagttcac tgatgtttgt gcttccaatg tcgctggcgg cagcggtaac tatccgcgta
    27481 ggttatcgtc tgggtcaggg ctcaacgctg gatgcgcaaa ccgctgcgcg gaccgggctt
    27541 atggtgggtg tctgtatggc aaccctgacg gccattttca cggtttcact gcgggagcaa
    27601 atcgccctgt tgtacaacga caatcccgag gttgtaacgc tggctgcgca tttgatgttg
    27661 ctggcggcgg tatatcagat ttctgactca atccaggtga ttggcagtgg gattttgcgt
    27721 ggttataaag atacgcgttc cattttctat atcaccttta cagcttactg ggtgctgggc
    27781 ttgccaagcg gctatattct ggcactgacc gatctggtcg ttgaacctat ggggccagca
    27841 ggcttctgga taggctttat tatcggcctg acatcggcag ccattatgat gatgctgcgt
    27901 atgcggttcc tgcaacgtat gccgtcagcc atcattctgc aacgagcgtc ccgctaataa
    27961 agacaaggcg caaccttcac gggttgcgcc tgtgttttta cgcaggctgg agcgttgcgc
    28021 caatcccgtc ttcgtctggc tgtaatttca gagcgttaca cagagtttgc cgactggcat
    28081 ctgttatctc aacgcgtata tccccgttaa cttccccttt cacattgatg aagtcaggtg
    28141 aaatttcttt cgcactggtt tgcaggcaaa tcgctccctc tccgcgcagg cgtttcactt
    28201 tcagttgcac atgcgttgcc gcaccagtga tcagccacag ttttccgcca ttcagattga
    28261 tgttaccaac aatgaaagtg acgctgtcac cttttaccgt actgccgtcg agaatcaaca
    28321 caccgccctg ctgtacagta atcgttgatt gttgcagctc tgcgccataa ccaataatca
    28381 ttgaagcgcc gtccagtaat gcaacactcc cggtcagttc aaccggtgcg cgatcttcct
    28441 ctacgacaaa attttcgtcc gcgctggtgg gtgacaacag gatcactgtt ccaccttgta
    28501 ttaccgcatt ttccagcgta ccggcatgca cttcagcccg cccccccaca ttcaccgaca
    28561 ggttttgcgt cttaccggaa ctgtagagct gaaggccatc cgtccccaga cgataaatgg
    28621 atccattctc aacaatagtg tcagagtctt caccaccgag attattcatg gtgccaccag
    28681 caagcaacgt atcactggct ttcccggcca gcataaaaac gccgccctga ttaatctcac
    28741 aattgctggc ttctccactt ggatacacca acaattcacc accttcatct accacgaccg
    28801 ctgaggttgt cccattaacc aacaggccgc cctcttgatc gagaatgatt ttttccgcgc
    28861 gatgtccttc cagtacacgc aggttaccgc cattttccag caacaaaccg caggcataac
    28921 cctgatcaac gctgaattca ccttcgggat ggcggccatt aaccgtagcg agcgtagaga
    28981 gactaattgc gcctccctcg gactgaacaa tatctgtggc aatggcaccg tcgctgaccg
    29041 atagcgtgcc attgttaccg tactgtgtgc atttggcgat cccaccggtt gccactttca
    29101 accagccagc ccccgtgacc acggtatttt cggctcttcc gccagcttca acaatcaact
    29161 cgccttcagt aagttgcgtc gcgccaagga cctcgccggt cttaacaatt tgcacattcc
    29221 cgtcgctcat caagtttttc gcatcagatc ccatgattta ttcctttgct gcatctgtgt
    29281 gcctttattg ctacctaagt gtaaaggcta cggaggattt atccacgaca gatttgagat
    29341 ggtggcaaac aactctgttt aaactctgat acacgaatta ttgggttgta tcagatgtaa
    29401 atgcgatcct gaataaaaat cgcccttgca aatcaacaaa atatagccaa tttgcttaaa
    29461 agctctgcaa acggtcgaaa tagcgcagaa aattacgttt tgcctcttgc cacctttcca
    29521 ctctgccgct aatattcgtc cccgttgtca cctacaacgt tgcgttcata gctcagttgg
    29581 ttagagcacc accttgacat ggtgggggtc gttggttcga gtccaattga acgcaccatc
    29641 ctgcgtccgt agctcagttg gttagagcac caccttgaca tggtgggggt cggtggttcg
    29701 agtccactcg gacgcaccag attttcttaa tctggtcttt tcctttttcc ctctgtttct
    29761 tctctgtatc caatacgtta aaagatttac actgtcttcg tatgcgttat cagaaggaga
    29821 atcgctatgg caactttgtt acaacttcat tttgctttta atggcccatt tggcgacgca
    29881 atggctgagc agcttaaacc acttgctgag tcgattaatc aggaacctgg ttttctgtgg
    29941 aaggtatgga cagaaagtga aaagaaccac gaagccggtg ggatctacct tttcactgat
    30001 gaaaaaagcg cccttgccta tctggaaaaa catactgccc gactgaaaaa tctcggcgtt
    30061 gaggaagttg tcgccaaagt tttcgatgtc aatgagccac ttagtcaaat caatcaggca
    30121 aaactcgcct gacagaattt aatcaagggc ggttagcgcc cttttcatcc ctgtctgaaa
    30181 tttctcaaat tctaaaaatc tcaaccaaac ttatctgata acactaaatt cgaaagaatg
    30241 cgtacaggta agtaacaatg aaaaaaattg ctattgtggg tgccgggcct acggggatct
    30301 acaccttatt ctcgcttcta cagcaacaaa ctccactttc tatttctatc ttcgagcagg
    30361 ctgacgaggc cggtgtcggg atgccataca gtgatgagga aaactcaaaa atgatgctgg
    30421 caaatattgc cagtattgaa ataccgccga ttaattgtac gtatctcgaa tggctacaaa
    30481 agcaagaagc cagccatctc cagcgttatg gcgttaaaaa agaaaccttg cacgatcgtc
    30541 agtttttacc gcgaattctg ctgggcgaat atttccgcga tcaattttta cgattagtag
    30601 accaggcacg acagcaaaaa tttgcagtgg ctgtttatga atcatgccag gttaccgatc
    30661 tgcaaattac aaatgctggc gtcatgctcg ctacaaatca ggatttaccc agcgagacgt
    30721 ttgatttagc ggtgattgcc acgggtcacg tctggcctga tgaagaagaa gcaaccagaa
    30781 cgtattttcc cagcccgtgg tcaggcctga tggaagcaaa ggtcgatgcg tgtaacgtgg
    30841 gtattatggg aacatccttg agcggactgg atgcggcaat ggcagtggct attcagcatg
    30901 gttcgttcat tgaagatgat aaacaacacg tcgtttttca ccgcgataac gcaagtgaaa
    30961 agctaaatat tacgttgatg tcgcgcacgg gtattttacc cgaagccgat ttctattgcc
    31021 ctattcccta cgagccctta cacatcgtca ccgatcaggc attaaatgct gagattcaaa
    31081 aaggtgaaga gggccttttg gatcgggtat ttagattgat agtagaggaa atcaagtttg
    31141 ctgatccaga ctggagccaa cgcatagcct tagagagcct gaatgtcgat tcctttgctc
    31201 aagcctggtt tgccgagcgc aaacaacgcg acccatttga ctgggcagaa aaaaatctcc
    31261 aggaagtcga acgcaataaa cgagaaaaac atactgttcc ctggcgttat gtcattctgc
    31321 gcctgcatga agccgtacag gaaattgttc ctcatctgaa tgaacacgac cataaacggt
    31381 tcagtaaagg ccttgccagg gttttcatcg ataattatgc ggcaatccct tcagagtcta
    31441 ttcgtcgact actggcctta cgtgaagcgg ggatctgaaa tccctgatgt tggcgaagat
    31501 tatacgttac agcaacccga agatattcgt ggacgcgtag cgttcggcgc gttgccctgg
    31561 ttgatgcacg accagccttt cgttcaggga cttacggcat gtgcagaaat tggtgaggcg
    31621 atggctcggg cggtcgtaaa gcctgcatcc cgtgctcgtc ggcgtctttc gtttgattaa
    31681 aggtaaagct atcagcactc aacaaactga tgctgttcat taagacgata gcggactccg
    31741 gttcgaatat tgttttcacc ttcaatggcg acggcgaagc gaactctttc gccgtcatga
    31801 tatgccagtg cggcgcttcc ccctagcccg agaataattg agtcgaccac gcccgtgctc
    31861 actaccgttg aattttcacc gctggcaatg atgtgtacat tgtcgccgct gttggcaatt
    31921 ctggcgtttg cgccaaagct ggcgatttgc accaggtcac cattactggc gacatgacac
    31981 cgttcaccca atgtacaaac gcgtactcgc attccggtat tcgcaatccg actactatct
    32041 ccagcgctgc ttatccgcga agaattaccg gttacagcaa cccgtgcgcg ttcaccggag
    32101 ctgccaatgt gactgttaaa gccgacactt ccaatccgca cgctataacc catactggca
    32161 atttgcgcgg catacccagc ggttgcgatt cgggcgttat cctcacaaca gccgatgcga
    32221 gcatgatcgt cgctgcgggc aaactgactg caaaatggta tctctccggg gtctgtggcc
    32281 agtttttgca tagaggaaac ttcctgatgc gcgaagtttt catccgccag ccattttttc
    32341 cacgcatact caaccagact ctccgcccag tccgtatatc cttcctcaat cagtgcatga
    32401 cgaatatcgg cataactacc gccgcgtggg aaatggcgca ggaaccagcg atacatcacc
    32461 gctccgatac gccattctcg caggtctgct cgtgtaatga tcattcgtta gtgccggtga
    32521 tagccatcga ccatgctttt aaaggtttcg tgtggcgtac gccccgtggt gcaaagataa
    32581 agatgaccga agataaaaaa gaggcttata aatgccagag caaaatgcgc ctgcaataac
    32641 cagtatctta cgccaggaaa cacatctccc acggcttgcg gatagagaca caacagcccc
    32701 gtcaatagta acaacggcag caatccatac atgacaccaa cataggcgac ctgctgtaag
    32761 ggattaaatt tagactgtgt tgttgccggg aaaggatgtt cttccccctg cataatgcca
    32821 aacaaataaa atcgcgtttg ttttgccgct cgttccagcc acccctgacg acgaatgcga
    32881 tagtggtgac cattatcccc aacggcattg atcagcacaa agccgagcca gcatgccagt
    32941 aacaaaaatc cgcaaacttc atgcaccgca accagacttt taaccgcagt tgcgcccacc
    33001 atcgcaaagt gatttatcag cccacttgcc agcaacaata caaagagtaa cgcattcgac
    33061 cagtgccata atctgaccgc tttgctgtaa agataaacct tctcaccatg atcggtggct
    33121 gatttcttca cgccacgagc acgaagcaag gcgtgcagcc ccagcaccaa ccacatccca
    33181 accagcaata ctccggcaat gatcaaccac accggccaaa attcgggagt gaactgtggc
    33241 acatagttcg ctaactggct ctgaaactgt tcagcatgtt gcgacgggtt catacatttt
    33301 cctttttaat caaatgttga ccgaaccggc gatacagatg cggttttccg gcaccaggta
    33361 gctgatattg atagtattta ttatcctgca accacgcctg aatttctggg ctatcttcgc
    33421 gaccaaagat caatgcgtgt tccggacagg cgctgacgca aatgggcgga aagccttttg
    33481 ccagtcggga ctcagcgcag aaatcacatt tatctgccac tttagtgaca gggttaaggt
    33541 agcgaaccag gtacggacaa gcgccaatgc aataactgca gccgatgcac tgcgattttt
    33601 ctacccgcac gatcccctgc tcatcacgcc acgatgcccc cgtcgggcat acgtcgatgc
    33661 acggagcatc ttcgcagtgc tgacatgact ggcgaaaaaa gtgatactgc gtttcgttgt
    33721 cgttatcggt gactggaatg tgtgctatcg ataaacggct tccctgagcg gggacgtggt
    33781 tagttttacg gcatgcgcgg gcgcaaatat tacagccatt gcaccgtgac tcgtcatgaa
    33841 tcatggcata ccgaacttcc ttttcttgcc tcgtgttcgc taacagagaa gacgcagaac
    33901 cagtaaaaaa gattactgtt cccatcccca gaacaaattt gcgtcgagtg aaggacatct
    33961 gattatcccc taatgcagtg gctgcaactg tccaccctat ccatgacatc tccctgaaat
    34021 ttcaggggtt ctgaaactgc tctacctcga accattcgca taagccataa gccttgccat
    34081 aggtcataaa tcgctcgcta aacaagtaca acaccgccgt cgatgactcc acctgccgta
    34141 tatcggcata ttcaggtgca acatccatcg cggcgatcgc ggcttcaatc tgcgcttcct
    34201 ggaagtaata tggcgcttgc atcagcatgg cgactttgta aggacgagga tacgtctggc
    34261 actcaaaacg aacggcgtgt gcaatggcgc gacaaatgtc ctgctccaca acttgcaaag
    34321 acatcgccgc gtagttttca ctcatggcct gcgttgagta atagtaatca tcctgacttc
    34381 ccgtcataca agcgatatcc gcgtagtctc cgttctgctt aatgctttcc agcaaaactg
    34441 acagttcctc ttctgcaacc gaatagggtg gcgagagaaa cacggtgcgc gccaccagct
    34501 gcccagccgc agaatgctgg cgaatgaact cagccaggat ttcagcaggt tctggttcgg
    34561 ttaccgtatc aacggggtga cgcatcccct gcaaccattg ccgcttcgcc tcgtcaactt
    34621 cgctgacttt cgctaacggc aattcatcct gatggttcat cttacccatt ccttacgcag
    34681 gcagtagatt gtgtgctgcc agatcggcgg caatatcttc cagccccaga cgctgcaatg
    34741 tttcgcgggt tggacaacca agctgtgggt cccagcccat ttctttgtag aacatcgtga
    34801 gcgaagcatg catatcgtca cgatccattt tgtcagtacc ttcagtaaag accgggatct
    34861 gcggatcctt gtcgaaaacc caggaacaga taagatcgtg ttcattacgc atatctttgg
    34921 tttgcatcgg ttttaccgtg taggcacgat gcaacgtaaa gatacgctct gcagctaaat
    34981 ctaatttttc ctgagtcatc tcttcgccgg tgatcgcttt gaagaatttg gcttcaagcg
    35041 ccagatcgcc ccggtaatta cggcttttca gcggcgaaac ggtcattggc cagacccagt
    35101 tgcacagagt gacggcgtta tgcaaacaga cccgcaacag cgaccacttt gcatatttaa
    35161 tttttgcgtc attgattggc gtgtagtttt tggtttcatc gtaagcatct tcagaaccaa
    35221 aaagttcttt cgccacttca cgttgcagtt tcaatggcaa gccggaacca ataaagttga
    35281 tatgggtatg cgtcatgcag tcacggttga acatacactt aacaatggaa ccgacctgcg
    35341 ctgacgcttc attggcatgg tgaaccggat agccaaacgg cgaccagagt ttatttttcg
    35401 cgtagcccca gtactcttta cccaaattcc agcgttctgc gatggcatat gaaccatcag
    35461 ccaggtgact cagctcaccc acacgatgcg ccagacggta gtaaaaatct ttaatgaagt
    35521 taacgtcacc cgcttccagt tgatcccagc gaatttctgc atactcttca gctggcagaa
    35581 cacgcttgaa cacacctttg ctgtaacaat aagtaaagtc gcgatgcaac tgcccgtagt
    35641 tacaccatag gccgtagtca tcgaacaaat tcagaccaac caggttacca atcacacgac
    35701 catcgtcttt atcttcaaaa tctttcggcc cgttcgggaa gatggtggta tggacaaagt
    35761 ttgctacaca agtgttacca cctgtgctgg gcacgccaaa ctctttcacc cgaggaatat
    35821 tcatttgggt catacaacgg atcgggcaag agtggcagcc gctcattttt actgtgtatt
    35881 tctctgccgc cggtcctaag tcaaaaacgg atttataggt acgaaagccg accgtattct
    35941 gattgcctgg cggaatttca cccgtttcaa tcggaccacc ttcagccgcg ccccaaaaca
    36001 gccctttacg tgctgtccag cgtgacttgg gatctgcgta cttggcacga catggttgtt
    36061 attcgcacca ataagttcag tcatcatgta atcattgaga cgcttcatct cctgacgatc
    36121 ggcaatgttg acccctttcg tcccttcaac cgcaatcgct ttcaggtttt tcgaacccat
    36181 tattgcgcca gttcccgcac cgccgctgtg gttacggcta ttcaacatgc cagagagagg
    36241 aataaggttt tccccagcct gaccaatagc cgccacacag gtttccgggc tggtcaatcg
    36301 acaaatttct tccgtcgttg cgcgcgtccc ttttccccat aagaaatcgg ctttttccag
    36361 gctaactttg tcatctttaa tcttcagcca taccggtgat ttcgccttcc cttcgataat
    36421 aatgacgtcg tatccagcga atttcatttg cgctgcaaaa aagccaccca tatgggcatc
    36481 gcgactaaat ttcctttggt aaaagtagaa agtgaggtga tatttacgcg agaactacag
    36541 ggggcaccag atccaggtaa tgggccggta gcaaagacta atttattcgc ttcatcgaaa
    36601 ggtttcgtgc ctagcggtac ttcgtcatac ataattttgt agccgaagcc catgccaccg
    36661 acaaaacttt taaacttact ggaatcttcg agggtaatat ttcctgtcgt gagattgact
    36721 cttaatatat tacctgtcca accgttagcc atgatttttt cctttgcaag attcacacag
    36781 taatatcttt ccactcgata atttttaacg cccctgttgg gcaggcgttt gcgcattcac
    36841 cgcataacac acatttcgag gattttttac tttcggtatt tacggtggcc atcatccacg
    36901 ggcatgccgt agtacaggcg ctacagccaa tacaacgttt atgatcgacg gtaatacagc
    36961 cttctttctg ctgccaggta atcgcaccaa tcgggcagac gttcatgcat tgcggctctt
    37021 tgcattgacg gcaggtgtcc gcggtatagt tgagatcgcc atacaggccg ccgccagagc
    37081 caaccccgtt gtcgccaaag aaataattgc gatggatttt gatacgggag aagaatgtcc
    37141 ctactgagcc atcgttgaag ttggtacaag agatttcaca gcggtggcaa cccgtgcatc
    37201 gcgctcgctg ggtcaccagc acccctttgg gtgtatttat caaccctact gtgccgctat
    37261 caatatcttc ctgtttgcaa cccaaaagtg acagtaacgc aggggcaatt gttagaccag
    37321 caaggccttt tcctgatata cgcaagaatt ctaatcgcgt taagccaata tctaatagtg
    37381 gacgatcaac cgggttcatt tatttcgcat cctcttcgca aacacagtgc tgacaattca
    37441 gcagcctgat attaagcatt caactaatac gctgctgact ctgtccccaa caccagatat
    37501 tttccctaac ccgatagtgt atgagaaaag atttaacgcc gataaactaa tcctttatgg
    37561 ttaggagaaa aaataatgtg attattctga gcccttaaca ttgatcgtta tcaattaaaa
    37621 ttacaaagca agaaattata agcgaactga tatttattat catttgaaat aaatttaact
    37681 taagcgacaa tcatttgtga cgttcattgc agaaaataaa aataacatta tcgctataaa
    37741 ttaaaataca taccgaaatt cgtataattt cagcagttgt tatttagaat gattaactgg
    37801 accgcaactg aagcaagaat atcaggatga agcttaccgc ctcatcctga atggtcattt
    37861 taccagtgcg ttaatgacgg tttccatcgc ctgcttttcc agctcactgc gctgaatgcg
    37921 tttattggct aatgctgtac gtaatacgcc tgcgataaca atatgctttg gttcctgccc
    37981 cagatcgcgc atttccagca caactttacc gacaactctg cacatttctc ggtacagttc
    38041 atcctctttt gtccgatttc ccattcttta tacctattta tcatgccaa
//
LOCUS       DAJTRC010000030        36893 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.030, whole genome shotgun sequence.
ACCESSION   DAJTRC010000030 DAJTRC010000000
VERSION     DAJTRC010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36893
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.030"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..622)
                     /gene="yhdJ"
                     /locus_tag="OO357_001641"
     CDS             complement(<1..622)
                     /gene="yhdJ"
                     /locus_tag="OO357_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5886622.1"
                     /translation="MRTGCEPTRFGNEAKTIIHGDAFAELKKLPTESVDLLFADPPYN
                     IGKNFDGLIEAWKEDLFIDWLFEVIAECHRVLKKQGSMYIMNSTENMPFIDLQCRKLF
                     TIKSRIVWSYDSSGVQAKKHYGSMYEPILMMVKDAKNYTFNGDAILVEAKTGSQRALI
                     DYRKNPPQPYNHQKVPGNVWDFPRVRYLMDEYENHPTQKPKALLKRI"
     gene            complement(708..1004)
                     /gene="fis"
                     /locus_tag="OO357_001642"
     CDS             complement(708..1004)
                     /gene="fis"
                     /locus_tag="OO357_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001465625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator Fis"
                     /protein_id="HCR5886623.1"
                     /translation="MFEQRVNSDVLTVSTVNSQDQVTQKPLRDSVKQALKNYFAQLNG
                     QDVNDLYELVLAEVEQPLLDMVMQYTRGNQTRAALMMGINRGTLRKKLKKYGMN"
     gene            complement(1030..1995)
                     /gene="dusB"
                     /locus_tag="OO357_001643"
     CDS             complement(1030..1995)
                     /gene="dusB"
                     /locus_tag="OO357_001643"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HCR5886624.1"
                     /translation="MRIGQYQLRNRLIAAPMAGITDRPFRTLCYEMGAGLTVSEMMSS
                     NPQVWESDKSRLRMVHIDEPGIRTVQIAGSAPKEMADAARINVESGAQIIDINMGCPA
                     KKVNRKLAGSALLQYPDVVKSILTEVVNAVDVPVTLKIRTGWAPEHRNCEEIAQLAED
                     CGIQALTIHGRTRACLFNGEAEYDSIRAVKQKVSIPVIANGDITDPLKARAVLDYTGA
                     DALMIGRAAQGRPWIFREIQHYLDTGELLPPLPLAEVKRLLCAHVRELHDFYGPAKGY
                     RIARKHVSWYLQEHAPNDQFRRTFNAIEDASEQLEALEAYFENFA"
     gene            complement(2324..3205)
                     /gene="prmA"
                     /locus_tag="OO357_001644"
     CDS             complement(2324..3205)
                     /gene="prmA"
                     /locus_tag="OO357_001644"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HCR5886625.1"
                     /translation="MPWIQLKLNTTGANAEDLSDALMEAGAVSITFQDTHDTPVFEPL
                     PGETRLWGDTDVIGLFDAETDMNDVVAILENHPLLGAGFAHKIEQLEDKDWEREWMDN
                     FHPMRFGERLWICPSWRDVPDENAVNVMLDPGLAFGTGTHPTTSLCLQWLDSLDLTGK
                     TVIDFGCGSGILAIAALKLGAAKAIGIDIDPQAIQASRDNAERNGVSDRLELYLPKDQ
                     PEEMKADVVVANILAGPLRELAPLISVLPVSGGLLGLSGILASQAESVCEAYADSFAL
                     DPVVEKEEWCRITGRKN"
     gene            complement(3217..4668)
                     /gene="panF"
                     /locus_tag="OO357_001645"
     CDS             complement(3217..4668)
                     /gene="panF"
                     /locus_tag="OO357_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709053.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/pantothenate symporter"
                     /protein_id="HCR5886626.1"
                     /translation="MQLEVILPLVAYLVVVFGISVYAMRKRSTGTFLNEYFLGSRSMG
                     GIVLAMTLTATYISASSFIGGPGAAYKYGLGWVLLAMIQLPAVWLSLGILGKKFAILA
                     RRYNAVTLNDMLFARYQSRLLVWLASLSLLVAFVGAMTVQFIGGARLLETAAGIPYET
                     GLLIFGISIALYTAFGGFRASVLNDTMQGLVMLIGTVVLLIGVVHAAGGLSNAVQTLQ
                     TIDPQLVTPQGADDILSPAFMTSFWVLVCFGVIGLPHTAVRCISYKDSKAVHRGIIIG
                     TIIVAILMFGMHLAGALGRAVIPDLTVPDLVIPTLMVKVLPPFAAGIFLAAPMAAIMS
                     TINAQLLQSSATIIKDLYLNIRPDQMQNETRLKRMSAVITLVLGALLLLAAWKPPEMI
                     IWLNLLAFGGLEAVFLWPLVLGLYWERANAKGALSAMIVGGVLYAVLATLNIQYLGFH
                     PIVPSLLLSLLAFLVGNRFGTSVPQATVLTTDK"
     gene            complement(4658..4900)
                     /locus_tag="OO357_001646"
     CDS             complement(4658..4900)
                     /locus_tag="OO357_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhdT family protein"
                     /protein_id="HCR5886627.1"
                     /translation="MDTRFVQAHKEARWALGLTLLYLAVWLVDAYLPGVAPGFTGFPR
                     WFEMACILTPLLFIGLCWAMVKFIYRDIPLEDDDAA"
     gene            complement(5009..6358)
                     /gene="accC"
                     /locus_tag="OO357_001647"
     CDS             complement(5009..6358)
                     /gene="accC"
                     /locus_tag="OO357_001647"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009483995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HCR5886628.1"
                     /translation="MLDKIVIANRGEIALRILRACKELGIKTVAVHSSTDRDLKHVLL
                     ADETVCIGPAPSVKSYLNIPAIISAAEITGAVAIHPGYGFLSENANFAEQVERSGFIF
                     IGPKAETIRLMGDKVSAIAAMKKAGVPCVPGSDGPLGDDMDKNRAIAKRIGYPVIIKA
                     SGGGGGRGMRVVRGDAELAQSISMTRAEAKAAFSNDMVYMEKYLENPRHVEIQVLADG
                     QGNAIYLAERDCSMQRRHQKVVEEAPAPGITPELRRYIGERCAKACVDIGYRGAGTFE
                     FLFENGEFYFIEMNTRIQVEHPVTEMITGVDLIKEQLRIAAGQPLSIKQEEVHVRGHA
                     VECRINAEDPNTFLPSPGKITRFHAPGGFGVRWESHIYAGYTVPPYYDSMIGKLICYG
                     ENRDVAIARMKNALQELIIDGIKTNVDLQIRIMNDENFQHGGTNIHYLEKKLGLQEK"
     gene            complement(6369..6839)
                     /gene="accB"
                     /locus_tag="OO357_001648"
                     /gene_synonym="fabE"
     CDS             complement(6369..6839)
                     /gene="accB"
                     /locus_tag="OO357_001648"
                     /gene_synonym="fabE"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HCR5886629.1"
                     /translation="MDIRKIKKLIELVEESGISELEISEGEESVRISRAAPAASFPVM
                     QQAYAAPMMQQPAQSNAAAPATVPSMEAPAAAEISGHIVRSPMVGTFYRTPSPDAKAF
                     IEVGQKVNVGDTLCIVEAMKMMNQIEADKSGTVKAILVESGQPVEFDEPLVVIE"
     gene            complement(7817..8791)
                     /locus_tag="OO357_001649"
     CDS             complement(7817..8791)
                     /locus_tag="OO357_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5886630.1"
                     /translation="MQALLLEQQDGKTLASVQTLDERRLPEGDVTVDVHWSSLNYKDA
                     LAITGKGKIIRNFPMIPGIDFAGTVRTSEDPRFHAGQEVLLTGWGVGENHWGGLAEQA
                     RVKGDWLVAMPQGLDARKAMIIGTAGFTAMLCVMALEDAGVRPQDGEIVVTGASGGVG
                     STAVALLHKLGYQVVAVSGRESTHEYLKSLGASGILPRDEFAESRPLEKQVWAGAIDT
                     VGDKVLAKVLAQMNYGGCVAACGLAGGFTLPTTVMPFILRNVRLQGVDSVMTPPERRA
                     QAWQRLVADLPESFYTQAAKEISLSEAPNFAEAIINNQIQGRTLVKVN"
     gene            8943..10883
                     /gene="csrD"
                     /locus_tag="OO357_001650"
     CDS             8943..10883
                     /gene="csrD"
                     /locus_tag="OO357_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase E specificity factor CsrD"
                     /protein_id="HCR5886631.1"
                     /translation="MRLTTKFSAFVTLLTGLTIFVTLLGCSLSFYNAIQYKFSHRVQA
                     VATAIDTHLVSNDFSALRPQITELMVSADIVRVDLLHGDKQVYTLARNGSYRPVGTSD
                     LFRELSVPLIKHPGMSLRLVYQDPMGNYFHSLMTTAPLTGAIGFIILMLFLAVRWLQR
                     QLAGQELLETRATRILNGERGSNVLGTIYEWPPRTSSALDTLLREIQNAREQHSRLDT
                     LIRSYAAQDVKTGLNNRLFFDNQLATLLEDQEKVGTHGIVMMIRLPDFNMLSDTWGHS
                     QVEEQFFTLTNLLSTFMMRYPGALLARYHRSDFAALLPHRTLKEAESIASQLIKAVDT
                     LPNNKMLDRDDMIHIGICAWRSGQDTEQVMEHAESATRNAGLQGGNSWAIYDDSLPEK
                     GRGNVRWRTLIEQMLSRGGPRLYQKPAVTREGQVHHRELMCRIFDGNEEVSSAEYMPM
                     VLQFGLSEEYDRLQISRLIPLLRYWPEENLAIQVTVESLIRPRFQRWLRDTLMQCEKS
                     QRKRIIIELAEADVGQHISRLQPVIRLVNALGVRVAVNQAGLTLVSTSWIKELNVELL
                     KLHPGLVRNIEKRTENQLLVQSLVEACSGTSTHVYATGVRSRSEWQTLIQRGVTGGQG
                     DFFASSQPLDTNVKKYSQRYSV"
     gene            11188..12231
                     /gene="mreB"
                     /locus_tag="OO357_001651"
     CDS             11188..12231
                     /gene="mreB"
                     /locus_tag="OO357_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000913396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreB"
                     /protein_id="HCR5886632.1"
                     /translation="MLKKFRGMFSNDLSIDLGTANTLIYVKGQGIVLNEPSVVAIRQD
                     RAGSPKSVAAVGHDAKQMLGRTPGNIAAIRPMKDGVIADFFVTEKMLQHFIKQVHSNS
                     FMRPSPRVLVCVPVGATQVERRAIRESAQGAGAREVFLIEEPMAAAIGAGLPVSEATG
                     SMVVDIGGGTTEVAVISLNGVVYSSSVRIGGDRFDEAIINYVRRNYGSLIGEATAERI
                     KHEIGSAYPGDEVREIEVRGRNLAEGVPRGFTLNSNEILEALQEPLTGIVSAVMVALE
                     QCPPELASDISERGMVLTGGGALLRNLDRLLMEETGIPVVVAEDPLTCVARGGGKALE
                     MIDMHGGDLFSEE"
     gene            12297..13400
                     /gene="mreC"
                     /locus_tag="OO357_001652"
     CDS             12297..13400
                     /gene="mreC"
                     /locus_tag="OO357_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HCR5886633.1"
                     /translation="MKPIFSRGPSLQIRLILAVLVALGIIIADSRLGTFSQIRTYMDT
                     AVSPFYFVSNAPRELLDGISQTLASRDQLELENRALRQELLLKNSELLMLGQYKQENA
                     RLRELLGSPLRQDEQKMVTQVISTVNDPYSDQVVIDKGSVNGVYEGQPVISDKGVVGQ
                     VVAVAKLTSRVLLICDATHALPIQVLRNDIRVIAAGNGCTDDLQLEHLPANTDIRVGD
                     VLVTSGLGGRFPEGYPVAVVSSVKLDTQRAYTVIQARPTAGLQRLRYLLLLWGADRNG
                     ANPMTPEEVHRVANERLMQMMPQVLPSPDAMGPKLPEPATGIAQPTPQQPATGNAATA
                     PVAPTQPAANRSPQRATPPQSGAQPPARAPGGQ"
     gene            13400..13888
                     /gene="mreD"
                     /locus_tag="OO357_001653"
     CDS             13400..13888
                     /gene="mreD"
                     /locus_tag="OO357_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001487551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HCR5886634.1"
                     /translation="MASYRSQGRWVIWLSFLIALLLQIMPWPDNLIVFRPNWVLLILL
                     YWILALPHRVNVGTGFVMGAILDLISGSMLGVRVLAMSIIAYLVALKYQLFRNLALWQ
                     QALVVMLLSLVVDIIVFWAEFLVINVSFRPEVFWSSVVNGVLWPWIFLLMRKVRQQFA
                     VQ"
     gene            13897..14490
                     /gene="yhdE"
                     /locus_tag="OO357_001654"
     CDS             13897..14490
                     /gene="yhdE"
                     /locus_tag="OO357_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphatase YhdE"
                     /protein_id="HCR5886635.1"
                     /translation="MTSLYLASGSPRRQELLAQLGVTFERIVTGIEEQRQPQESAQQY
                     VVRLAREKAQAGVAQTAQDLPVLGADIIVILNGEVLEKPRDAEHAAQMLRKLSGQTHQ
                     VMTAVALADSQHILDCLVVTDVTFRTLTDEDIAGYVASGEPLDKAGAYGIQGLGGCFV
                     RKINGSYHAVVGLPLVETYELLSNFNALREKRDKHDG"
     gene            14480..15949
                     /gene="rng"
                     /locus_tag="OO357_001655"
     CDS             14480..15949
                     /gene="rng"
                     /locus_tag="OO357_001655"
                     /EC_number="3.1.26.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006818051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease G"
                     /protein_id="HCR5886636.1"
                     /translation="MTAELLVNVTPSETRVAYIDGGILQEIHIEREARRGIVGNIYKG
                     RVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVAGEEQKQFTVRDISELVRQGQD
                     LMVQVVKDPLGTKGARLTTDITLPSRYLVFMPGASHVGVSQRIESESERERLKKVVAE
                     YCDEQGGFIIRTAAEGVGEAELASDAAYLKRVWTKVMERKKRPQTRYQLYGELALAQR
                     VLRDFADAELDRIRVDSRLTYEALLEFTSEYIPEMTSKLEHYTGRQPIFDLFDVENEI
                     QRALERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLDDTIFNTNIEATQAIARQ
                     LRLRNLGGIIIIDFIDMNNEDHRRRVLHSLEQALSKDRVKTSVNGFSALGLVEMTRKR
                     TRESIEHVLCNECPTCHGRGTVKTVETVCYEIMREIVRVHHAYDSDRFLVYASPAVAE
                     ALKGEESHSLAEVEIFVGKQVKVQIEPLYNQEQFDVVMM"
     gene            16017..19817
                     /gene="yhdP"
                     /locus_tag="OO357_001656"
     CDS             16017..19817
                     /gene="yhdP"
                     /locus_tag="OO357_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709041.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA2 domain-containing protein YhdP"
                     /protein_id="HCR5886637.1"
                     /translation="MRRLPGILLLTGAALVVIAALLVSGLRIALPHLNAWRPEILNKI
                     ESATGMPVEASQLSASWQNFGPTLEAHDIRAELKDGGEFSVKRVTLALDVWQSLLHMR
                     WQFRDLTFWQLRFRTNTPITSGGSDDSLEASHISDLFLRQFDHFDLRDSEVSFLTPSG
                     QRAELAIPQLTWLNDPRRHRAEGLVSLSSLTGQHGVMQVRMDLRDDEGLLSNGRVWLQ
                     ADDIDLKPWLGKWMQDNIALETAQFSLEGWMTIDKGDVTGGDVWLKQGGASWLGEKQT
                     HTLSVDNLTAHITRENPGWQFSIPDTRITMDGKPWPSGALTLAWIPEQDVGGKDNKRS
                     DELRIRASNLELAGLEGIRPLAAKLSPALGDVWRSTQPSGKINTLALDIPLQAADKTR
                     FQASWSDLAWKQWKLLPGAEHFSGTLSGSVENGLLTASMKQAKMPYETVFRAPLEIAD
                     GQATISWLNNDKGFQLDGRNIDVKAKAVHARGGFRYLQPANDEPWLGILAGISTDDGS
                     QAWRYFPENLMGKDLVDYLSGAIQGGEADNATLVYGGNPQLFPYKHNEGQFEVLVPLR
                     NAKFAFQPDWPALTNLDIELDFINDGLWMKTDGVNLGGVRASNLTAVIPDYSKEKLLI
                     DADIKGPGKAVGPYFDETPLKDSLGATLQELQLDGDVNARLHLDIPLNGELVTAKGEV
                     TLRNNSLFIKPLDSILKNLSGKFSFINGDLQSEPLTASWFNQPLNVDFSTKEGAKAYQ
                     VAVNLNGNWQPAKTGVLPEAVNEALSGSVAWDGKVGIDLPYHAGATYNVELNGDLNNV
                     SSHLPSPLAKPAGEPLAVNVKVDGNLNSFELTGQAGADNHFNSRWLLGQKLTLDRAIW
                     AADSKTLPPLPEQSGVELNMPPMNGAEWLALFQKGAAESVGGAASFPQHITLRTPMLS
                     LGNQQWNNLSIVSQPTANGTLVEAQGREINATLAMRNNAPWLANIKYLYYNPSVAKTR
                     GDSTPSSPFPTTERINFRGWPDAQIRCTECWFWGQKFGRIDSDITISGDTLTLTNGLI
                     DTGFSRLTADGEWVNNPGNERTSLKGKLRGQKIDAAAEFFGVTTPIRQSSFNVDYDLH
                     WRKAPWQPDEATLNGIIHTQLGKGEITEINTGHAGQLLRLLSVDALMRKLRFDFRDTF
                     GEGFYFDSIRSTAWIKDGVMHTDDTLVDGLEADIAMKGSVNLVRRDLNMEAVVAPEIS
                     ATVGVAAAFAVNPIVGAAVFAASKVLGPLWSKVSILRYHISGPLDDPQINEVLRQPRK
                     EKAQ"
     gene            19973..21418
                     /gene="tldD"
                     /locus_tag="OO357_001657"
     CDS             19973..21418
                     /gene="tldD"
                     /locus_tag="OO357_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease TldD"
                     /protein_id="HCR5886638.1"
                     /translation="MSLNLVSEQLLAANGLKHQDLFAILGQLAERRLDYGDLYFQSSY
                     HESWVLEDRIIKDGSYNIDQGVGVRAISGEKTGFAYADQISLLALEQSAQAARTIVRD
                     SGDGKVQTLGAVEHSPLYTSVDPLQSMSREEKLDILRRVDKVAREADKRVQEVTASLS
                     GVYELILVAATDGTLAADVRPLVRLSVSVLVEEDGKRERGASGGGGRFGYEFFLADLD
                     GEVRADAWAKEAVRMALVNLSAVAAPAGTMPVVLGAGWPGVLLHEAVGHGLEGDFNRR
                     GTSVFSGQVGELVASELCTVVDDGTMVDRRGSVAIDDEGTPGQYNVLIENGILKGYMQ
                     DKLNARLMGMTPTGNGRRESYAHLPMPRMTNTYMLPGKSTPQEIIESVEYGIYAPNFG
                     GGQVDITSGKFVFSTSEAYLIENGKVTKPVKGATLIGSGIETMQQISMVGNDLKLDNG
                     VGVCGKEGQSLPVGVGQPTLKVDNLTVGGTA"
     gene            complement(21546..22475)
                     /gene="aaeR"
                     /locus_tag="OO357_001658"
     CDS             complement(21546..22475)
                     /gene="aaeR"
                     /locus_tag="OO357_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator AaeR"
                     /protein_id="HCR5886639.1"
                     /translation="MERLKRMSVFAKVVEFGSFTAAARQLQMSVSSISQTVSKLEDEL
                     QVKLLNRSTRSIGLTEAGRIYYQGCRRMLHEVQDVHEQLYAFNNTPIGTLRIGCSSTM
                     AQNVLAGLTAKMLKEYPGLSANLVTGIPAPDLIADGLDVVIRVGALQDSSLFSRRLGA
                     MPMVVCAAKSYLTQYGIPEKPADLSSHSWLEYSVRPDNEFELIAPEGISTRLIPQGRF
                     VTNDPMTLVRWLTAGAGIAYVPLMWVINEINRGELEILLPRYQSDPRPVYALYTEKDK
                     LPLKVQVVINSLTDYFVEVGKLFQEMHGRGKEK"
     gene            22658..22861
                     /locus_tag="OO357_001659"
     CDS             22658..22861
                     /locus_tag="OO357_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AaeX family protein"
                     /protein_id="HCR5886640.1"
                     /translation="MSLFPVIVVFGLSFPPIFFELLLSLAIFWLVRRVLVPTGIYDFV
                     WHPALFNTALYCCLFYLISRLFV"
     gene            22869..23801
                     /gene="aaeA"
                     /locus_tag="OO357_001660"
     CDS             22869..23801
                     /gene="aaeA"
                     /locus_tag="OO357_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeA"
                     /protein_id="HCR5886641.1"
                     /translation="MKTLIRKFSRTAITVVLVILAFIAIFNAWVYYTESPWTRDARFS
                     ADVVAIAPDVSGLITQVNVHDNQLVKKGQILFTIDQPRYQKALEEAQADVAYYQVLAQ
                     EKRQEAGRRNRLGVQAMSREEIDQANNVLQTVLHQLAKAQATRDLAKLDLERTVIRAP
                     ADGWVTNLNVYTGEFITRGSTAVALVKQNSFYVLAYMEETKLEGVRPGYRAEITPLGS
                     NKVLKGTVDSVAAGVTNASSTRDDKGMATIDSDLEWVRLAQRVPVRIRLDNQQENIWP
                     AGTTATVVVTGKQDRDESQDSFFRKMAHRLREFG"
     gene            23807..25774
                     /gene="aaeB"
                     /locus_tag="OO357_001661"
     CDS             23807..25774
                     /gene="aaeB"
                     /locus_tag="OO357_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="p-hydroxybenzoic acid efflux pump subunit AaeB"
                     /protein_id="HCR5886642.1"
                     /translation="MGIFSIANQHIRFAVKLATAIVLALFVGFHFQLETPRWAVLTAA
                     IVAAGPAFAAGGEPYSGAIRYRGFLRIIGTFIGCIAGLVIIIAMIRAPLLMILVCCIW
                     AGFCTWISSLVRIENSYAWGLAGYTALIIVITIQPEPLLTPQFAVERCSEIVIGIVCA
                     IMADLLFSPRSIKQEVDRELESLLVAQYQLMQLCIKHGDGEVVDKAWGDLVRRTTALQ
                     GMRSNLNMESSRWARANRRLKAINTLSLTLITQSCETYLIQNTRPELITDTFREFFDT
                     PVETAQDVHKQLKRLRRVIAWTGERETPVTIYSWVAAATRYQLLKRGVISNTKINATE
                     EEILQGEPEVKVESAERHHAMVNFWRTTLSCILGTLFWLWTGWTSGSGAMVMIAVVTS
                     LAMRLPNPRMVAIDFIYGTLAALPLGLLYFLVIIPNTQQSMLLLCISLAVLGFFLGIE
                     VQKRRLGSMGALASTINIIVLDNPMTFHFSQFLDSALGQIVGCVLAFTVILLVRDKSR
                     DRTGRVLLNQFVSAAVSAMTTNVARRKENHLPALYQQLFLLMNKFPGDLPKFRLALTM
                     IIAHQRLRDAPIPVNEDLSAFHRQMRRTADHVISARSDDKRRRYFGQLLEELEIYQEK
                     LRIWQAPPQVTEPVHRLAGMLHKYQHALTDS"
     gene            25866..26138
                     /locus_tag="OO357_001662"
     CDS             25866..26138
                     /locus_tag="OO357_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="barstar family protein"
                     /protein_id="HCR5886643.1"
                     /translation="MNIYAFDFDEIESQEDFYRDFSQTFGLAKDKVRDLDSLWDVLMN
                     DVLPLPLEIEFVHLGEKTRRRFGALILLFDEAEEELEGHLRFNVRH"
     gene            complement(26194..26457)
                     /gene="yhcN"
                     /locus_tag="OO357_001663"
     CDS             complement(26194..26457)
                     /gene="yhcN"
                     /locus_tag="OO357_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid stress response protein YhcN"
                     /protein_id="HCR5886644.1"
                     /translation="MKIKTTVAALSVLSVLSFGAFAADSIDAAQAQNREAIGTVSVSG
                     VASSPMDIREMLNKKAEEKGATAYQITEARSGDTWHATAELYK"
     gene            complement(26822..27292)
                     /gene="argR"
                     /locus_tag="OO357_001664"
     CDS             complement(26822..27292)
                     /gene="argR"
                     /locus_tag="OO357_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001257852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ArgR"
                     /protein_id="HCR5886645.1"
                     /translation="MRSSAKQEELVKAFKALLKEEKFSSQGEIVAALQEQGFDNINQS
                     KVSRMLTKFGAVRTRNAKMEMVYCLPAELGVPTTSSPLKNLVLDIDYNDAVVVIHTSP
                     GAAQLIARLLDSLGKAEGILGTIAGDDTIFTTPANGFTVKDLYEAILELFDQEL"
     gene            27727..28665
                     /gene="mdh"
                     /locus_tag="OO357_001665"
     CDS             27727..28665
                     /gene="mdh"
                     /locus_tag="OO357_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000861594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5886646.1"
                     /translation="MKVAVLGAAGGIGQALALLLKTQLPSGSELSLYDIAPVTPGVAV
                     DLSHIPTAVKIKGFSGEDATPALEGADVVLISAGVARKPGMDRSDLFNVNAGIVKNLV
                     QQVAKNCPKACIGIITNPVNTTVAIAAEVLKKAGVYDKNKLFGVTTLDIIRSNTFVAE
                     LKGKQPGEVEVPVIGGHSGVTILPLLSQVPGVSFTEQEVADLTKRIQNAGTEVVEAKA
                     GGGSATLSMGQAAARFGLSLVRALQGEQGVVECAYVEGDGQYARFFSQPLLLGKNGVE
                     ERKSIGTLSAFEQNALEGMLDTLKKDIALGKEFVNK"
     gene            complement(28727..29794)
                     /gene="degS"
                     /locus_tag="OO357_001666"
     CDS             complement(28727..29794)
                     /gene="degS"
                     /locus_tag="OO357_001666"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane-stress sensor serine
                     endopeptidase DegS"
                     /protein_id="HCR5886647.1"
                     /translation="MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETP
                     ASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDAD
                     QIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGN
                     PYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTL
                     SFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQSGGI
                     DQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVV
                     VMRDDKQLTLQVTIQEYPATN"
     gene            complement(29884..31231)
                     /gene="degQ"
                     /locus_tag="OO357_001667"
                     /pseudo
     CDS             complement(29884..31231)
                     /gene="degQ"
                     /locus_tag="OO357_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417701.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine endoprotease DegQ"
     gene            complement(31385..31783)
                     /locus_tag="OO357_001668"
     CDS             complement(31385..31783)
                     /locus_tag="OO357_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1043 family protein"
                     /protein_id="HCR5886648.1"
                     /translation="MTWEYALIGLVVGIIIGAVAMRFGNRKLRQQQALQYELEKNKAE
                     LDEYREELVSHFARSAELLDTMAHDYRQLYQHMAKSSSSLLPELSAEANPFRNRLAES
                     EASNDQAPVQVPRDYSEGASGLLRTGAKRD"
     gene            31977..33104
                     /gene="zapE"
                     /locus_tag="OO357_001669"
     CDS             31977..33104
                     /gene="zapE"
                     /locus_tag="OO357_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HCR5886649.1"
                     /translation="MQSVTPTSQYLKALNEGSHQPDDVQKEAVSRLEIIYQELINSRP
                     PAPRTSGLMARVGKLWGKREDTKHTPVRGLYMWGGVGRGKTWLMDLFYQSLPGERKQR
                     LHFHRFMLRVHEELTALQGQTDPLEIIADRFKAETDVLCFDEFFVSDITDAMLLGGLM
                     KALFTRGITLVATSNIPPDELYRNGLQRARFLPAIDAIKQHCDVMNVDAGVDYRLRTL
                     TQAHLWLSPLNDETRAQMDKLWLALAGVKRENSPTLEINHRPLATMGVENQTLAVSFT
                     TLCVDARSQHDYIALSRLFHTVMLFDVPVMTRLMESEARRFIALVDEFYERHVKLVVS
                     AEVPLYEIYQGERLKFEFQRCLSRLQEMQSEEYLKREHLAG"
     gene            33323..33751
                     /gene="rplM"
                     /locus_tag="OO357_001670"
     CDS             33323..33751
                     /gene="rplM"
                     /locus_tag="OO357_001670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HCR5886650.1"
                     /translation="MKTFTAKPETVKRDWYVVDATGKTLGRLATELARRLRGKHKAEY
                     TPHVDTGDYIIVLNADKVAVTGNKRTDKVYYHHTGHIGGIKQATFEEMIARRPERVIE
                     IAVKGMLPKGPLGRAMFRKLKVYAGNEHNHAAQQPQVLDI"
     gene            33767..34159
                     /gene="rpsI"
                     /locus_tag="OO357_001671"
     CDS             33767..34159
                     /gene="rpsI"
                     /locus_tag="OO357_001671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000829824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HCR5886651.1"
                     /translation="MAENQYYGTGRRKSSAARVFIKPGNGKIVINQRSLEQYFGRETA
                     RMVVRQPLELVDMVEKLDLYITVKGGGISGQAGAIRHGITRALMEYDESLRSELRKAG
                     FVTRDARQVERKKVGLRKARRRPQFSKR"
     gene            34554..35192
                     /gene="sspA"
                     /locus_tag="OO357_001672"
     CDS             34554..35192
                     /gene="sspA"
                     /locus_tag="OO357_001672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stringent starvation protein A"
                     /protein_id="HCR5886652.1"
                     /translation="MAVAANKRSVMTLFSGPTDIYSHQVRIVLAEKGVSFEIEHVEKD
                     NPPQDLIDLNPNQSVPTLVDRELTLWESRIIMEYLDERFPHPPLMPVYPVARGESRLY
                     MHRIEKDWYTLMNTIINGSASEADAARKQLREELLAIAPVFGQKPYFLSDEFSLVDCY
                     LAPLLWRLPQLGIEFSGPGAKELKGYMTRVFERDSFLASLTEAEREMRLGRS"
     gene            35198..35695
                     /gene="sspB"
                     /locus_tag="OO357_001673"
     CDS             35198..35695
                     /gene="sspB"
                     /locus_tag="OO357_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor"
                     /protein_id="HCR5886653.1"
                     /translation="MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPME
                     YARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGT
                     MFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPA
                     LRVVK"
     gene            complement(35738..>36893)
                     /gene="dcuC"
                     /locus_tag="OO357_001674"
     CDS             complement(35738..>36893)
                     /gene="dcuC"
                     /locus_tag="OO357_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuC"
                     /protein_id="HCR5886654.1"
                     /translation="RIAGLGLSIMAVGGYARYMERIGASCAMVSLLSRPLKLIRSPYI
                     ILSATYVIGQIMAQFITSASGLGMLLMVTLFPTLVSLGVSRLSAVAVIATTMSIEWGI
                     LETNSIFAAQVAGMKIATYFFHYQLPVASCVIISVAISHFFVQRAFDKKDKNINHEQA
                     EQKTLDNVPPLYYAILPVMPLILMLGSLFLAHVGLMQSELHLVVVMLLSLTVTMFVEF
                     FRKHNLRETMDDVQAFFDGMGTQFVNVVTLVVAGEIFAKGLTTIGTVDAVIRGAEHSG
                     LGGIGVMIIMALVIAICAIVMGSGNAPFMSFASLIPNIAAGLHVPAVVMIMPMHFATT
                     LARAVSPITAVVVVTSGIAGVSPFAVVKRTAIPMAVGFVVNMIATITLFY"
BASE COUNT         8913 a         9239 c         9738 g         9003 t
ORIGIN      
        1 taatgcgttt cagtaaggct ttcggttttt gcgtcgggtg gttctcatat tcatccatca
       61 aataacgcac gcgcggaaaa tcccagacgt tacccggtac tttttgatga ttgtatggct
      121 gtggaggatt tttgcgataa tcgatcaacg cgcgctgcga tccggttttg gcttctacca
      181 gaatagcatc accgttgaat gtgtagttct ttgcgtcttt caccatcatc aggatgggtt
      241 cgtacatgga gccgtagtgt tttttcgcct gtactccaga actgtcatat gaccagacga
      301 tgcgactttt gatggtaaaa agcttgcggc actggagatc gataaagggc atgttttccg
      361 tactgttcat aatgtacatg ctgccctgct ttttcagaac gcggtggcac tctgcaatca
      421 cttcaaacag ccagtcgata aacagatctt ctttccaggc ttcgatcaga ccatcaaaat
      481 ttttaccgat gttatacggt gggtcggcaa agagcagatc gacgctttca gtaggtagct
      541 ttttaagttc ggcaaaggca tcaccgtgaa taatggtctt agcttcatta ccaaaccggg
      601 tcggttcaca tcctgttctc atggtcactc cctttgtgac acctataaaa aaggcgcttc
      661 cccatgccga gtagcgcctt tttaatcaag catttagcta acctgaatta gttcatgccg
      721 tattttttca attttttacg cagcgtacca cggttgatgc ccatcatcag cgcagcacgg
      781 gtctggttac cacgggtgta ttgcatcacc atgtccaaca ggggctgttc tacttcagcc
      841 agtaccagct catagaggtc attcacatcc tgaccattca gttgagcaaa atagttcttc
      901 agtgcctgtt taaccgagtc acgcaggggt ttttgggtta cctgatcctg agagttaacg
      961 gtagaaacgg tcagtacgtc agaatttacg cgttgttcga acatagttct gtcagctctt
     1021 tatttctgtt tacgcaaaat tttcgaagta tgcctccaac gcctccagct gttcgctggc
     1081 atcctcaatg gcgttgaatg tgcgccgaaa ctggtcattt ggagcgtgtt cctggagata
     1141 ccaggaaacg tgtttacgtg caattcggta cccttttgcc ggaccataaa agtcatgcag
     1201 ttcccgaacg tgcgcgcaaa gcaagcgctt aacctctgcc aaaggcagtg ggggcagcaa
     1261 ctccccagtg tccagataat gctggatttc ccgaaagatc cagggtcttc cctgagctgc
     1321 gcggcctatc atcagggcat ccgcccctgt atagtcgagc acagctctgg ctttaagcgg
     1381 gtcagtaatg tcgccattcg cgataaccgg aatggaaact ttctgcttaa ctgcccgaat
     1441 actgtcgtac tcagcttctc cattgaacaa acaggcgcgt gtacggccat gaatggtcag
     1501 agcctgaatg ccacagtctt cagccagttg ggcaatctct tcgcagttac ggtgttccgg
     1561 tgcccagccg gtgcgaatct tcagggtaac aggaacgtcc actgcattga cgacctcggt
     1621 aaggatcgat ttaacgacat ccgggtactg caagagggct gaacctgcga gcttgcgatt
     1681 cactttttta gccgggcaac ccatattgat atcaataatc tgggcaccgc tttccacgtt
     1741 aatacgtgct gcatctgcca tttctttcgg agcgctacca gcaatttgca cggtgcgaat
     1801 accgggttca tcaatgtgca ccatccgtaa acgagatttg tcgctttccc aaacctgtgg
     1861 gttagaagac atcatctcgg atactgtcaa tccggctccc atctcgtagc acaacgtccg
     1921 aaaaggtctg tctgtaatgc cagccatggg cgctgcgatc aggcgatttc tgagctgata
     1981 ttgtccgatg cgcatgagtt aagaaatgac catactgtga ctgcaaggcg gcgtatatta
     2041 cgcatttttt gcacgagatg aaaggccaaa ctttgaccaa tcctctgaga tggatcaaag
     2101 aattgcattt aaaatgagcg tggtgcgata attactcata aaaatcatca tattagaaaa
     2161 tagtgactaa aatttacact caaagaaatt tgagtaagtt ctcaattttt ctttatgaat
     2221 gaaaattttg gcacgcaaat ttgcgtaaat aatcggcaaa tttacgtgcc ttttgtgagc
     2281 ttgctcgcac ttcgccccgc gtcaccctac ggcgatgcga aggtcaattc ttacgaccgg
     2341 taatacggca ccactcttct ttttccacga ccgggtccag tgcgaagcta tcggcataag
     2401 cttcacaaac gctctctgcc tggcttgcca gaataccgga aaggcccagc aaaccgcctg
     2461 aaactggcag gacgctgatt aacggtgcca gttcacgtaa tgggcctgca aggatgttag
     2521 cgaccaccac gtcggctttc atttcttctg gctgatcttt cggtaagtag agttccagac
     2581 ggtcagaaac gccattacgt tcggcgttat cgcggctggc ctgaatcgcc tgcggatcga
     2641 tatcaatacc aatggctttt gctgcaccca gtttcagcgc cgcgatcgcc agaatgccgg
     2701 aaccacagcc aaagtcgatg actgttttac cggttaaatc gaggctgtcg agccattgca
     2761 ggcacagaga ggtggttgga tgggtacccg taccaaacgc cagccctgga tctaacatca
     2821 cgttgacggc gttttcgtcc ggcacatcac gccagctagg gcagatccac agtcgttcac
     2881 caaagcgcat cgggtggaaa ttatccatcc attcgcgctc ccagtcttta tcttctagtt
     2941 gttcgatttt atgcgcgaag cctgcgccga gcagcggatg gttttccaga atcgccacca
     3001 cgtcgttcat atcggtttca gcgtcgaaca gaccaatcac atcggtgtcg ccccacaggc
     3061 gcgtttcgcc cggcagcggc tcaaatactg gcgtatcgtg ggtatcctga aaagtgatag
     3121 aaacggcacc cgcttccatc agcgcatcgc taagatcttc cgcgttcgcg ccggtggtgt
     3181 tcagtttcag ttggatccaa ggcatggcaa aactctttat ttatcagtag tcaaaacggt
     3241 agcttgcggg acggatgtac cgaaacggtt tccgaccagg aaagccagca aacttagtag
     3301 taacgagggc acgatagggt ggaagcccag gtactgaata ttcagcgtcg cgagtacggc
     3361 atacagcacg ccgccaacga tcatcgcact tagcgcgcct ttggcgttgg cgcgttccca
     3421 gtaaagaccc agcaccagcg gccacaggaa aacggcttcc agcccaccga aggccagcaa
     3481 attcagccag atgatcattt ctggcggctt ccaggcggca agcagcagca acgcgccgag
     3541 aactaacgta attaccgccg acatccgctt cagacgcgtc tcgttttgca tttgatccgg
     3601 acggatattc agatagagat ctttaatgat cgtagcggaa ctttgcagca gttgggcgtt
     3661 aattgtcgac atgatcgcag ccataggtgc agccagaaag atcccggcag caaaaggtgg
     3721 cagcactttt accattaacg tcgggatcac cagatctggt acggtgagat cggggatcac
     3781 tgcacgacct aacgctccgg ccaggtgcat accgaacatc aggattgcga ctataatcgt
     3841 accgatgatg atcccccggt gtactgcttt gctgtcttta taagagatac agcgcaccgc
     3901 agtatgcggc aggccaatca cgccaaagca caccagtacc cagaacgacg tcataaaggc
     3961 aggcgacaga atatcgtcag cgccttgtgg cgtaaccagt tgcggatcga tggtttgcaa
     4021 ggtctgtact gcgttgctta agccgccagc ggcatgtacc acgccaataa gcagcacaac
     4081 ggtgccaatc agcatcacaa gcccttgcat ggtgtcgttc agcacgctgg cgcgaaagcc
     4141 accaaaggcg gtatataacg caatgctgat accaaaaatc agcagcccgg tttcataagg
     4201 aatacccgcc gcggtttcca gcaggcgcgc accgccgata aactgcacgg tcatcgcacc
     4261 aacgaacgca accagcaaac tcaaactcgc cagccacacc agaagacgac tctggtagcg
     4321 ggcaaacagc atatcgttca gcgtcactgc attgtagcgg cgcgcaagaa tcgcaaactt
     4381 cttgccgaga ataccgagtg aaagccagac agcaggaagc tgaatcatcg ccagcaatac
     4441 ccagcccagc ccgtatttat aagcagctcc tggcccgccg ataaacgaac tggcactgat
     4501 ataggtcgcg gtgagagtca tcgccagcac aataccgccc atagagcggc tgccgaggaa
     4561 atactcatta aggaaggtgc cggtgctccg tttacgcatc gcataaaccg agataccgaa
     4621 caccaccacc agataggcga ccagcggtag aattacttca agctgcatcg tcatcctcca
     4681 gtgggatatc gcgatagata aatttcacca tcgcccagca cagtccaata aacagtagcg
     4741 gcgtcaggat gcaggccatc tcaaaccagc gcggaaagcc ggtaaaaccg ggggcaacgc
     4801 caggtaagta agcgtctact aaccaaactg ccagatacaa aagggtcagc cccagcgccc
     4861 agcgggcctc tttatgggcc tgaacaaaac gagtgtccat tttttgtccc tgatgggtga
     4921 agaaagcgag gattgtacct tatgggggtt gtcgatcccc agtaataaaa aaggccggaa
     4981 aatccggcct tttgacgctt ttgcagtctt atttttcctg aagaccgagt tttttctcca
     5041 gatagtggat gttagtgcca ccatgctgga agttctcgtc attcatgatg cggatctgca
     5101 gatcaacgtt ggttttgata ccgtcgatga tcagctcctg cagcgcattc ttcatgcggg
     5161 caatcgccac gtcacggttt tcaccgtagc aaatcagctt accgatcatt gagtcatagt
     5221 acggcggtac ggtgtagccc gcgtagatat gagactccca acgtacgcca aaaccgccag
     5281 gtgcgtggaa acgggtgatt ttgcctgggc ttggcaggaa ggtgttcgga tcttcggcgt
     5341 tgatacgaca ttccaccgca tggccgcgaa cgtgaacttc ttcttgcttg atcgacagcg
     5401 gttgaccggc agcgatacgc agctgttctt tgatcaggtc aacgccggtg atcatttctg
     5461 taaccgggtg ttctacctga atacgggtgt tcatttcgat gaaatagaac tcgccgtttt
     5521 cgaacaggaa ctcgaaagta cctgcaccgc gatagccgat atcaacacac gctttagcgc
     5581 aacgttcgcc gatgtagcga cgcagttccg gggtaatgcc cggtgctggt gcttcttcga
     5641 ccactttctg gtggcggcgc tgcatggagc agtcacgttc cgccagatag atagcgttgc
     5701 cctgaccgtc agccagtacc tgaatctcga cgtggcgagg attctccagg tatttctcca
     5761 tgtaaaccat atcgttgctg aaagcagctt tcgcttccgc acgggtcatg gagatggatt
     5821 gtgccagttc agcgtcgccg cgcactacgc gcataccgcg accgccgccg ccgccggagg
     5881 ctttgataat caccggataa ccaatgcgtt tagcaatggc gcggttttta tccatatcgt
     5941 cgcccagcgg gccgtcagaa ccaggtacgc aaggaacgcc cgcttttttc atcgccgcga
     6001 ttgcggatac tttgtcgccc atcaggcgaa tggtttctgc tttcgggccg atgaagataa
     6061 agccggagcg ttcaacctgc tcggcaaagt tggcgttctc ggagaggaag ccgtaacccg
     6121 gatggattgc tactgcgccg gtgatttcag cggcgctgat gattgccggg atgttcagat
     6181 aactttttac tgacggagca gggccaatac agaccgtttc atctgccagt aatacgtgtt
     6241 ttagatcgcg atccgtgctg gagtgcacag cgacagtctt gatgcccagt tctttacagg
     6301 cacgaagaat acgcaatgca atctcgccgc ggttggcaat aacaatttta tccagcatgt
     6361 tcgcctcgtt actcgatgac gaccagcggc tcgtcaaatt ctaccggttg tccactttcg
     6421 accagaattg ctttcacggt accggatttg tccgcttcga tctggttcat cattttcatg
     6481 gcttcaacga tgcacagggt atcgcccacg ttgactttct gacccacttc gatgaacgct
     6541 tttgcgtccg ggcttggggt gcggtagaaa gtaccaacca tcggggaacg tacgatgtga
     6601 ccactgattt ccgctgctgc tggcgcttcc atggaaggaa cggtcgccgg agcggctgcg
     6661 ttagattgag ctggctgctg catcattggt gcagcgtaag cttgttgcat cacagggaaa
     6721 cttgcggcag gagctgcacg gctaatgcgt actgactctt cgccttcaga aatttccagt
     6781 tcggagatgc ctgattcttc aaccagctcg atcagttttt taatcttacg aatatccatg
     6841 agtgggttcc gtactctttg tttagtgtga ttgtgacagg cgtttcaccg ccgtctgtaa
     6901 agcgtatgcc ccgacagctg tatgcatagc gataaattcc agcaggccgg gacgccggtc
     6961 tattttgccc cagagtgtca atgttagact tgacggacat tgtgcagcga tctgctgcta
     7021 tcctccggca aaaaacaaaa tataccttcg ttgcgccaat gtcacctttt ccgcagcgca
     7081 aaaactgcgt cagggaggac gaggtcgcac attataacga tttcgtagca attggcagct
     7141 aaatactggt cttatcaggg aatataatca acagctaaca tgtaaataac cttcaacacc
     7201 gtgtaatttg caacaagccg cacaattcac gaaattagcg ccatcatcga cggaacttct
     7261 tataacgtaa ggtgaaaagc tgaaaagcca gccctgcgaa gatgaggggc tgcaatgaga
     7321 taatcttcac agaccacaaa taatgtaggg atgccaggat cgcgacaaga tagacgacat
     7381 tacgcagcgg ttgctgggga ttacccaatt ttcactgcat cgtctggata gatgtgaatg
     7441 ctaaagcacg caaaatcaac caactgacaa ttctcagcat taaataggtt cgagtggtta
     7501 atttgtagtc aaataaaatc agattattca ccccatggta ccagaaatca gtaacaggtt
     7561 aagcgtagtg tcgcccaggc aaagcaccat aaacctaaca ggcgacggag gcgtatcaat
     7621 aatggctgtt tagaggagta tcaatgcgca tcaatttgtg gtcagtcgaa atcatgactt
     7681 tccagtttgg atgatgttgt acaacttcct gaccaaggtg ttcgacgaaa gctgtccggc
     7741 taaccgcttt tcccgttaac cgattgaata atgatgtcaa aatgagttcc ctctcttatt
     7801 attccttgct aaatggttag ttaaccttca ccagcgtgcg accctggatc tggttattaa
     7861 tgatggcctc ggcaaagttc ggtgcctctg acagagatat ctcttttgcc gcctgggtat
     7921 agaatgattc cggtaaatcg gcgaccagtc gctgccaggc ttgtgcgcgg cgttctggtg
     7981 gcgtcattac tgaatccacc ccttgcaaac ggacattacg cagaataaat ggcatgaccg
     8041 tggttggcag agtaaaacca cccgccagac cacaggccgc cacgcagccg ccgtaattca
     8101 tttgcgccag cacttttgcc agcactttgt cgccaacagt gtcaattgcc ccagcccaga
     8161 cttgtttttc cagaggacgg gattcggcaa actcatcacg agggagaata ccgctggcac
     8221 ctaaactttt cagatattca tgggtacttt cgcgaccgga aacggcaacg acctgataac
     8281 ccaacttatg cagcagcgcc acggcggtac tgccgacgcc accactggca cccgtcacga
     8341 caatctcacc gtcctgcggg cgaacaccgg catcttccag cgccatcaca cacagcatgg
     8401 cggtaaaacc ggcagtaccg ataatcattg ctttacgcgc gtccagccct tgcggcatgg
     8461 caaccagcca gtcacctttc actcgcgcct gctccgccag cccaccccag tggttttcac
     8521 caacgcccca gccagtgagt aacacctcct gaccggcatg aaaacgcgga tcttcgctgg
     8581 tgcgtacagt tccggcaaaa tcgatcccag gaatcatcgg aaaattacgg atgatttttc
     8641 ccttaccggt aatcgccagc gcatctttat agttcaggct cgaccagtga acatcgaccg
     8701 tgacatcgcc ctccggcagg cgacgttcgt ccagagtctg tactgatgcg agagttttgc
     8761 cgtcctgctg ttctaaaagt aacgcctgca taagtggtcc tcatgtgcat gatggattgg
     8821 aaaataattt ctgaagacta tactcgctaa tggaaaagca aagccgatga agcgcaataa
     8881 attgcgagat aaatctgatt tgctagtatg cccgcttcct cactatcgga gttaacacaa
     8941 ggatgagatt aacgacgaaa ttttcggcct ttgttacgct gctcaccggg ttaacaattt
     9001 ttgtgacttt gctgggctgt tcgctaagtt tctacaacgc cattcagtat aagtttagtc
     9061 atcgcgttca ggcggtggcg acggcgattg atacccacct tgtgtcgaat gacttcagcg
     9121 cattaaggcc acaaattacc gaattaatgg tgtcagcaga tatcgttcgt gtagacctgc
     9181 tgcatggtga taaacaggtt tataccctgg ccagaaatgg tagttatcgt ccggttggca
     9241 ccagcgatct gtttcgtgaa ctgagcgttc cgttgataaa gcatccgggg atgtcgttgc
     9301 gtctggttta tcaggatccg atgggcaact atttccattc gttgatgacc accgcgccgc
     9361 tcacgggggc gattggcttt atcattctta tgctctttct ggcggtacgc tggttacaac
     9421 ggcaacttgc cgggcaagaa ttgctggaaa cccgggctac tcgtatctta aacggtgagc
     9481 gtggctctaa tgtgttggga accatctatg aatggccgcc cagaaccagc agtgcgctgg
     9541 atacgctgct tcgtgaaatt cagaacgcac gcgaacaaca cagccgtctt gatacgctga
     9601 tccgctctta tgccgcacag gacgtgaaaa ccggcctcaa taaccgactc tttttcgata
     9661 atcagttagc aacgttactg gaagatcagg agaaagtagg tacccacggg atcgtgatga
     9721 tgattcgtct gccggatttc aatatgttga gcgatacctg ggggcacagc caggttgaag
     9781 aacagttctt cactctgacg aatctgctgt cgacatttat gatgcgctac cctggcgcac
     9841 tgctggcgcg ttaccaccgc agtgattttg ctgcgctgtt accgcaccgg acgttaaaag
     9901 aggcagagag catagccagt cagttaatca aagccgtcga taccttgccg aacaataaaa
     9961 tgctcgatcg cgacgatatg atccacattg gtatctgtgc ctggcgtagt ggtcaggata
    10021 ccgagcaggt aatggaacat gcagagtctg ccacgcgtaa tgcgggattg cagggtggca
    10081 atagctgggc tatttatgat gactcgttgc ctgaaaaagg acgcggtaat gttcgctggc
    10141 gtacgcttat cgagcaaatg ctcagtcgcg gcggcccgcg cctttatcaa aaaccggcgg
    10201 ttactcgcga aggtcaggtt catcatcgcg aactcatgtg ccgcatcttc gatggtaatg
    10261 aagaggttag ctcggcggag tatatgccga tggtcttgca gtttggctta tcggaagagt
    10321 atgaccgtct gcaaatcagc cgtcttattc cactattgcg ttactggcca gaggaaaatc
    10381 tggcgattca ggttaccgtt gagtcgctga ttcgtccgcg ttttcagcgt tggctgcgcg
    10441 atacgttaat gcaatgtgaa aaatcacaac gaaaacgcat aattattgaa cttgcagagg
    10501 ccgatgtagg tcaacatatc agtcgtttac aacctgttat tcgtttagtg aatgctttag
    10561 gggtacgggt agccgtcaac caggctggtt tgacgctggt aagcaccagt tggatcaaag
    10621 aacttaatgt tgagttactc aagctccatc cggggctggt caggaacatt gagaagcgaa
    10681 cggagaacca gctgctggtt caaagcctgg tggaagcctg ctccgggacc agcacccacg
    10741 tttacgccac cggcgtgcgt tcgcgaagcg agtggcagac cctgattcag cgcggtgtta
    10801 caggcgggca aggggatttt ttcgcgtcct cacagccact tgatactaac gtgaaaaaat
    10861 attcacaaag atactcggtt taacctgccg tttaatccgt tttcacgtag aataacgcgc
    10921 gctgcgtctc atgggagtgt gcttgtctgc tcgccagatt gttgcagcac atatgcagat
    10981 gaatgacctt acgcggttgc aaacaggcga ggaatgctgc tgatgcatta agcctttctg
    11041 gactcaggca gagatttgta acaaaggaaa cgaactgcac taattttcac cgtagcagat
    11101 gatttttgcg ccttgtcgct gctgcgtgtg gttggtaaag taagcggatt ttcttttccg
    11161 ccccagcttt caggattatc ccttagtatg ttgaaaaaat ttcgtggcat gttttccaat
    11221 gacttgtcca ttgacctggg tactgcgaat accctcattt atgtaaaagg acaaggcatc
    11281 gtattgaatg agccttccgt ggtggccatt cgtcaggatc gtgccggttc accgaaaagc
    11341 gtagctgcag taggtcatga cgcgaagcag atgctgggcc gtacgccggg caatattgct
    11401 gccattcgcc caatgaaaga cggcgttatc gccgacttct tcgtgactga aaaaatgctc
    11461 cagcacttca tcaaacaagt gcacagcaac agctttatgc gtccaagccc gcgcgttctg
    11521 gtttgtgtac cggttggcgc gacccaggtt gaacgccgcg caattcgtga atccgcgcag
    11581 ggcgctggtg cccgtgaagt cttcctgatt gaagaaccga tggctgccgc aattggtgct
    11641 ggcctgccgg tttctgaagc gaccggttct atggtggttg atatcggtgg tggtaccact
    11701 gaagttgctg ttatctcctt gaacggcgtg gtttactcct cttccgtgcg cattggtggt
    11761 gatcgtttcg acgaagctat catcaactat gtgcgtcgta attacggttc tctgatcggt
    11821 gaagccaccg cagaacgtat caagcacgaa atcggttcgg cttacccggg cgatgaagtc
    11881 cgtgaaatcg aagttcgtgg ccgtaacctg gcagaaggtg ttccacgcgg ttttaccctg
    11941 aactccaacg aaatcctcga agcactgcag gaaccgctga ccggtattgt gagcgcggta
    12001 atggttgcac tggaacagtg cccgccggaa ctggcttccg acatctccga gcgcggcatg
    12061 gtgctcaccg gtggtggcgc actgctgcgt aaccttgacc gtttgttaat ggaagaaacc
    12121 ggcattccag tcgttgttgc tgaagacccg ctgacctgtg tggcgcgcgg tggcggcaaa
    12181 gcgctggaaa tgatcgacat gcacggcggc gacctgttca gcgaagagta atcggatgca
    12241 ggcaggggaa gcgtctgttt accctgcctg gtctgatacg agaatacgca taacttatga
    12301 agccaatttt tagccgtggc ccgtcgctac agattcgcct tattctggcg gtgctggtgg
    12361 cgctcggcat tattattgcc gacagccgcc tggggacgtt cagtcaaatc cgtacttata
    12421 tggataccgc cgtcagtcct ttctactttg tttccaatgc tcctcgtgaa ttgctggatg
    12481 gcatatcgca gacgctggcc tcgcgtgacc aactagaact tgaaaaccgg gcgttacgtc
    12541 aggaactgtt gctgaaaaac agtgaactgc tgatgcttgg acaatacaaa caggagaacg
    12601 cgcgtctgcg cgagctgctg ggttccccgc tgcgtcagga tgagcagaaa atggtgactc
    12661 aggttatctc cacggttaac gatccttata gcgatcaagt tgttatcgat aaaggtagcg
    12721 ttaatggcgt ttatgaaggc cagccggtca tcagcgacaa aggtgttgtt ggtcaggtgg
    12781 tggccgtcgc taaactgacc agtcgcgtgc tgctgatttg tgatgcgacc cacgcgctgc
    12841 caatccaggt gctgcgcaac gatatccgcg taattgcagc cggtaacggt tgtacggatg
    12901 atttgcagct tgagcatctg ccagcgaata cggatattcg tgttggtgat gtgctggtga
    12961 cctccggtct gggcggtcgt ttcccggaag gctatccggt cgcggttgtc tcttccgtaa
    13021 aactcgatac ccagcgcgct tatactgtga tccaggcgcg tccgactgca ggactgcaac
    13081 gtttgcgtta tctgctgctg ctgtggggcg cggatcgtaa cggcgctaac ccgatgacgc
    13141 cggaagaggt gcatcgtgtt gctaatgaac gtctgatgca gatgatgccg caggtattgc
    13201 cttcgccaga cgcgatgggg ccaaagttac ctgaaccggc aacggggatc gctcagccga
    13261 ctccgcagca accggcgaca ggaaatgcag ctactgcgcc tgttgcgccg acacagcccg
    13321 ctgctaatcg ctctccacaa agggctacgc cgccgcaaag tggtgctcaa ccgcctgcgc
    13381 gtgcgccggg agggcaatag tggcgagcta tcgtagccag ggacgctggg taatctggct
    13441 ctctttcctc attgcgctgt tgctgcaaat catgccctgg ccggataacc tgattgtttt
    13501 ccggccaaac tgggtgttac ttatcttgtt gtattggatc ctggccttgc cccatcgcgt
    13561 aaatgtgggc acaggttttg tgatgggtgc catactggat ctgatcagcg gctcgatgct
    13621 tggcgtacgc gtattggcga tgagcatcat tgcttacctg gtggcgctga aataccagct
    13681 tttccgcaac ctcgcattat ggcagcaggc gctggtcgtc atgttgcttt cgctggtggt
    13741 ggatattatt gttttctggg cagagttttt agtgattaac gtctctttca gaccggaagt
    13801 gttctggagt agtgtagtca atggggtgct ctggccgtgg attttcttgc tgatgcgtaa
    13861 agtccgtcag cagtttgcag tgcaataaag gtttctatga cttctctgta tttagcttcc
    13921 ggttctccgc gtcgtcagga gttacttgcg caacttggcg tgacctttga acgtattgtt
    13981 acgggcattg aggagcagcg tcagccgcag gagagcgcgc agcagtatgt tgtgcgtctg
    14041 gcacgcgaga aagcacaggc gggtgtcgcg caaacggcac aggatctccc ggtgctgggt
    14101 gcggatatta tcgttatcct gaacggagaa gtgctcgaga aaccgcgcga cgcagagcat
    14161 gcggcgcaga tgttgcgcaa attatcgggt cagacccatc aggtgatgac ggcagtggcg
    14221 ttggccgaca gccagcacat tctcgattgc ctggtggtca ccgatgtgac tttcagaacg
    14281 ttaacagacg aagacatcgc gggctatgtc gccagcggtg aaccgttaga taaagcaggt
    14341 gcatacggta ttcaggggct gggtggctgt tttgtcagga agataaatgg cagctatcac
    14401 gccgtagtcg gcttaccgct ggttgaaacg tatgaattat taagtaattt taacgcactg
    14461 cgtgagaaaa gggataaaca tgacggctga attgttagta aacgtaacgc cttcggaaac
    14521 gcgagtggcg tatattgatg gcggtattct gcaggaaatt catattgaac gtgaggcgcg
    14581 acgcggaata gtaggcaata tctacaaggg tcgtgtaagt cgtgtacttc cgggtatgca
    14641 ggcggctttt gtagatattg ggctggataa agccgcgttt cttcatgcat ccgacatcat
    14701 gccgcatacc gaatgtgtgg cgggtgaaga acaaaagcaa ttcacggtgc gcgacatctc
    14761 ggaactggtt cgtcaggggc aagatctgat ggtgcaggtg gtgaaagatc cgcttggcac
    14821 taaaggtgcg cgcctgacca ccgatatcac gctgccttct cgctatctgg tatttatgcc
    14881 aggggcttct cacgttgggg tttcccaacg aattgaaagc gaatcagaac gtgaacgtct
    14941 gaaaaaagtg gtcgcagagt attgcgacga gcagggcggg tttatcatcc gcaccgcagc
    15001 ggaaggggtt ggcgaggctg aactggcctc cgatgccgct tatctgaaac gcgtctggac
    15061 caaagttatg gagcgtaaaa agcgcccgca gacccgttat cagctgtacg gcgaactggc
    15121 gctggcgcag cgtgttctgc gtgattttgc cgacgccgaa ctggaccgca ttcgcgttga
    15181 ctcacgcctg acttacgaag cattgctgga gtttacctcg gagtacattc ccgagatgac
    15241 cagcaagctg gagcattaca ccggacgcca gccgattttc gatctctttg atgtcgaaaa
    15301 cgaaatccag cgagcgttgg aacgcaaagt agaactgaaa tccggtggtt atctgattat
    15361 cgaccagacc gaagcgatga ccaccgtgga catcaatacc ggagcgtttg tcggtcatcg
    15421 caatctggac gacactattt tcaataccaa tattgaagcg acgcaggcta tcgctcgcca
    15481 gttacggttg cgtaatctgg gcgggattat cattattgat ttcatcgata tgaataatga
    15541 agatcaccgc cgccgcgtgc tgcattcgct ggagcaggcg ttgagcaaag accgggtgaa
    15601 aaccagcgtt aatggttttt cggcgctggg gctggtggag atgacgcgta aacgcacccg
    15661 cgaaagcatt gagcacgtac tgtgtaacga atgcccaacc tgccacggtc gcggaacggt
    15721 gaaaaccgtg gaaacggtat gctatgaaat catgcgcgag attgttcgtg tccaccatgc
    15781 ttacgactcc gaccgtttcc tggtctatgc ttctccggca gtagccgaag ccttgaaagg
    15841 cgaagagtca cactcgctgg cggaagtgga aattttcgtt ggcaaacagg ttaaagtaca
    15901 aattgaaccg ctctataacc aggagcagtt tgacgtcgta atgatgtaaa cagatgctgg
    15961 gccgccatcc ggcaaagggt ttttgagtca catttttagc agacaaggag tgacgggtga
    16021 ggcgattgcc ggggatttta ctgcttactg gagccgcgct cgttgtgatc gctgccctgc
    16081 tggttagcgg cctgcgtatt gctttaccgc atcttaacgc ctggcgtccg gaaatcctca
    16141 acaaaataga atccgcgact ggcatgccgg tagaagccag tcagctctca gccagctggc
    16201 agaattttgg cccgacgctt gaagcacacg acatccgtgc agaactaaaa gatggcggcg
    16261 aattttcggt taaacgcgtt actctggcgc tggatgtctg gcagagcctg ttacatatgc
    16321 gctggcagtt tcgcgacctc actttctggc agctgcgctt tcgcaccaac actcctatca
    16381 ccagcggtgg tagcgatgac agtctggaag ccagtcacat cagcgatctg tttcttcgtc
    16441 aatttgacca tttcgatctc cgcgacagtg aagtcagttt tctgacgcca tccggtcagc
    16501 gcgccgagct ggcgatccca caactcacct ggctgaacga tccacgtcga caccgtgcgg
    16561 aaggcctggt aagcctctcc agccttaccg gacagcacgg cgtgatgcag gtgcgcatgg
    16621 atttgcgcga tgatgagggg ttgttaagca atggtcgcgt ctggctacag gcggatgaca
    16681 tcgacctgaa gccgtggctc ggtaaatgga tgcaggacaa tattgctctg gaaacggcac
    16741 agttctccct tgaaggctgg atgacgatcg acaaaggcga tgtaaccggc ggtgacgtct
    16801 ggctgaaaca gggcggtgcc agctggttgg gcgagaagca aacgcatacg ctgtcggtgg
    16861 ataatctgac cgcgcatatt acgcgtgaaa atccgggctg gcagttctct attcccgata
    16921 cacggatcac gatggacggc aaaccctggc cgagcggagc attgacgctg gcctggatac
    16981 cggaacagga cgttggcggc aaagacaata aacgcagtga cgaactccgg attcgcgcta
    17041 gtaatctgga gctggcaggc ctggagggca tacgcccgct ggccgcgaaa ctttcacctg
    17101 cactgggtga tgtttggcgc tccacacaac cgagcggcaa gattaacact ctggcgctgg
    17161 atatcccgct tcaggcggca gacaagaccc gttttcaggc atcgtggagc gatctggcct
    17221 ggaagcaatg gaaattatta ccgggtgcgg aacacttctc cgggacgctt tccggcagcg
    17281 ttgaaaatgg tttgcttacc gcgtcgatga agcaggcaaa gatgccttac gaaacggtat
    17341 tccgtgcgcc actggaaatc gccgacggcc aggcaactat aagctggctg aacaatgaca
    17401 aaggtttcca gctggatggg cgtaatattg acgttaaagc caaagccgtc catgcgcgcg
    17461 gcggttttcg ttacctgcaa cctgctaacg atgaaccctg gctgggtatt ctggctggca
    17521 tcagtaccga tgatggttca caagcctggc gctatttccc ggaaaacttg atgggtaaag
    17581 acctggttga ttatttaagt ggtgcgattc agggcggtga agcggataac gcgacgctgg
    17641 tttatggtgg caatccgcaa ctcttcccct ataaacacaa cgaaggtcag tttgaagtgc
    17701 tggtgccgct gcgcaacgcg aagtttgcct tccagccgga ctggcctgca ttaactaacc
    17761 ttgatattga actggacttt attaacgacg gtttatggat gaaaaccgat ggcgttaatc
    17821 tgggcggcgt gcgcgcgagt aatctcaccg cagtgatccc tgactactca aaagaaaaac
    17881 tgctgattga cgctgacatt aaaggtccgg gtaaagccgt tggcccttac tttgatgaga
    17941 caccgctgaa agattctctg ggtgcgaccc tgcaagaact ccagctcgac ggcgatgtga
    18001 atgctcgctt acatcttgat atcccgctga acggcgaact ggtaaccgcg aaaggtgaag
    18061 tgacgctgcg taataacagt ctgtttatca aaccactcga cagcatcctg aaaaatttga
    18121 gcggtaaatt cagctttatc aatggcgatc tgcaaagtga accactgaca gcaagctggt
    18181 ttaatcagcc gttgaacgtg gatttttcca ccaaagaagg ggcaaaagcc taccaggttg
    18241 cggtgaatct caacggtaac tggcaaccgg cgaaaaccgg cgttctgcct gaagcggtga
    18301 acgaagcatt gagtggcagc gtggcgtggg atggtaaagt gggcattgat ctgccttatc
    18361 atgctggcgc gacctataac gtagagctga acggcgatct gaataatgtg agtagtcact
    18421 taccttcacc gttagccaaa cctgcgggtg aaccactagc ggtaaacgtt aaggttgatg
    18481 gcaatctcaa cagctttgaa ttaaccggac aggctggtgc ggataaccat ttcaatagcc
    18541 gctggttgct tggtcaaaag ctgacgctcg atcgtgctat ttgggcggca gacagtaaaa
    18601 cgctcccgcc gttgccggaa caaagtggcg ttgaacttaa tatgccgccg atgaatggtg
    18661 ccgagtggct ggccctgttc cagaaaggcg ctgcggagag tgtcggtggt gcagcgagtt
    18721 tcccacaaca cataacgtta cgtacgccta tgttgtcact gggaaatcag caatggaata
    18781 acctgagtat tgtttcgcaa ccgacggcaa atggcaccct ggttgaggcg caagggcgtg
    18841 aaatcaacgc cacgctagcg atgcgtaata acgcgccgtg gctggcgaat atcaaatatc
    18901 tttattacaa cccgagcgtg gcgaaaactc gtggtgattc aacgccgtca tcacctttcc
    18961 cgacaacgga gcgcattaac ttccgtggct ggccggacgc acaaatacga tgcacagagt
    19021 gctggttctg ggggcaaaaa ttcggtcgca ttgacagtga tatcaccatt tctggcgata
    19081 cgttaacgct gaccaatgga ctgattgata ctggtttctc gcggcttact gccgatggtg
    19141 aatgggttaa taatccgggg aatgaacgta cctcgctgaa aggaaaactg cgtgggcaga
    19201 aaattgatgc cgccgcagaa ttttttggtg tcacgacgcc catacgccag tcgtcattta
    19261 atgtggatta cgatttacac tggcgcaaag caccctggca gccagatgaa gcgacgttga
    19321 atggcatcat tcatactcaa ctgggtaaag gcgaaattac cgaaatcaat accggacatg
    19381 ccgggcaatt gctgcgctta ttgagcgtag atgccctgat gcgtaagctg cgttttgatt
    19441 tcagagacac ttttggcgaa gggttctatt ttgactccat tcgcagcacc gcgtggatta
    19501 aagacggcgt tatgcacacc gacgacacgc tggtggatgg cctggaggcg gatatcgcca
    19561 tgaaagggtc ggtaaatctg gtacgccgcg acctgaatat ggaagcggtt gtcgcaccag
    19621 agatttctgc gacggtgggc gtggctgcgg cttttgcggt taaccccatt gttggcgcgg
    19681 cagtgtttgc cgccagtaaa gtgctggggc cgctgtggag caaagtctcc attttgcgct
    19741 atcacatttc gggtccgctg gacgatccgc aaatcaacga agtgttgcgc caaccgcgta
    19801 aagaaaaagc gcaatgattt gacgagggcg cgtaattgcc ccaatctcat atgataatcg
    19861 ttgccaaagg ccaacgagcc ggaacataac tgtaggccgg atagggcgtt cacgccgcat
    19921 ccggcagccg taaaaaatcc tctactgcag taactaacga gtagcaaaaa cgatgagtct
    19981 gaacctggta agtgaacaat tgctagcggc gaacggcctg aaacatcagg acttgttcgc
    20041 gatcctcggt caactggccg aacgtcgcct tgattatggc gatctctatt ttcagtcgag
    20101 ctatcacgaa tcctgggttt tagaagaccg cattattaaa gatggttctt acaacatcga
    20161 tcagggcgtt ggcgtgcgtg caatcagcgg tgaaaaaacc ggatttgctt acgctgacca
    20221 aatcagcctg ctggcgctgg aacagagtgc gcaagcggcg cgcaccatcg tccgtgatag
    20281 tggtgatggc aaagtacaga cgctgggcgc ggtagagcat agcccgttgt atacctcggt
    20341 agatccgctg caaagcatga gccgtgaaga gaagctggat atcctgcgtc gcgtcgataa
    20401 ggttgctcgc gaagcggaca agcgcgtaca ggaagtgact gccagcctca gtggtgtcta
    20461 tgaattaatt ctggttgcgg ccaccgacgg cacgctagcg gcggatgtcc gtccgctggt
    20521 gcgtctttcc gtgagcgttc tggtcgaaga agatggcaaa cgcgaacgcg gtgccagtgg
    20581 cggcggcggt cgttttggtt atgagttctt ccttgccgat cttgacggcg aagtccgtgc
    20641 ggatgcatgg gcaaaagaag cagtgcgtat ggcgctggtc aatctttctg ccgttgctgc
    20701 accagcgggc accatgccgg tagtacttgg cgcaggttgg ccgggcgtgc tgttgcatga
    20761 agcggtaggt cacggtctgg aaggcgactt caaccgccgt ggcacttcag tatttagtgg
    20821 acaggtcggg gagctggtgg cttcagaact gtgtaccgtg gttgatgacg gcacgatggt
    20881 cgatcgccgt ggttcggtgg cgattgatga tgaaggtacg ccaggccagt acaacgtgct
    20941 gattgagaac ggcattctga aaggctacat gcaggataaa ctcaacgcgc gtttgatggg
    21001 gatgacgccg actggcaacg gtcgccgtga atcctacgcc catctgccca tgccgcgtat
    21061 gaccaacacc tatatgctgc cgggtaaatc gaccccgcaa gaaattattg aatccgttga
    21121 gtacggtatc tatgcaccga actttggtgg cggtcaggtg gatatcacct ccggcaaatt
    21181 cgttttctcc acttcagaag catatctgat tgaaaacggt aaagtaacga agccggtgaa
    21241 aggcgcaacg ttgattggtt ccggtatcga aaccatgcag cagatttcga tggttggcaa
    21301 cgacctgaaa ctggataacg gcgtgggtgt ctgcggtaaa gaagggcaaa gtttgccggt
    21361 tggcgtgggc cagccaacgt tgaaagtcga taacctgact gttggcggta ctgcgtaata
    21421 atttcattat tttcagaagg ataattaatc tttctacgtg cacgaacggt cccctcgctc
    21481 ctctggggtt agggtgaggg gaacccgttg gcacaggttt gtagaacgta acagtacaat
    21541 atgaattact tctctttccc gcgcccgtgc atctcctgaa acaatttacc gacctcaaca
    21601 aaataatccg tcagcgagtt gatcacgacc tgtaccttca gcggcagctt atctttttcg
    21661 gtatataacg cataaaccgg gcgtggatct gactgataac gcggcagcag gatctccagc
    21721 tccccacgat tgatctcgtt gatcacccac atcagcggca cgtaggcgat cccggcaccc
    21781 gccgttagcc agcgcaccag cgtcatcgga tcattagtca caaatctccc ttgcgggatc
    21841 aggcgagtcg agatcccttc cggtgcaatc agttcaaatt cattgtcggg ccgcacgctg
    21901 tattcaagcc atgaatggct acttaaatcg gcgggttttt ccggtatgcc gtattgtgtg
    21961 agatagcttt tcgcggcgca caccaccatt ggcatcgcgc caagacggcg ggaaaacagg
    22021 ctggaatcct gcaacgcgcc gacgcggatc accacatcca gaccgtcggc aatcaggtcg
    22081 ggggctggaa ttccggtaac cagattggcg ctcaaacctg ggtattcttt cagcattttg
    22141 gctgtcagcc cggcgagaac attttgtgcc atagttgaag aacagccaat gcgtagcgtc
    22201 ccgatggggg tgttattgaa ggcatacagt tgctcatgaa catcctgcac ttcatgaagc
    22261 atacgacggc agccctggta gtaaattcta ccggcttcgg tcaggccaat gctgcgtgtg
    22321 ctacggttta acagctttac ctgcaactca tcttccagtt ttgacaccgt ctgactgatg
    22381 gacgaaacgc tcatctgtag ctgtctggcg gcggcggtaa aagagccaaa ttcaactact
    22441 ttggcaaaca ccgacatgcg ttttagtcgt tccattattc actctgactt aaaagtgatt
    22501 tagatcacat aatatagata acagcataac agttacgcta atatattaaa tatcaatcta
    22561 cagcaatgtt gctctcgccc ggctttccat gcccttctct gcggcgacag atgctgaaaa
    22621 taataacgcc tgctctctct atacaaccaa ggtcaacatg agtctgtttc ccgttatcgt
    22681 ggtgtttggg ctgtccttcc caccgatatt ttttgaattg cttttatcac tggcgatttt
    22741 ctggctggtg cgccgggtac ttgtgccaac aggtatctac gactttgtct ggcatccggc
    22801 gttgttcaac accgcgctct attgctgctt gttttatttg atatcgcgac tgttcgtttg
    22861 aggttgaagt gaaaacacta ataagaaaat tctcccgtac ggccatcacg gtcgtattag
    22921 tcattctggc cttcatcgca atttttaatg cctgggtcta ttacaccgaa tccccctgga
    22981 cgcgtgacgc gcgctttagc gctgacgtcg ttgcgatcgc gccggacgtt tctggactca
    23041 ttacccaggt gaatgttcat gataaccagc tggtgaaaaa aggacagata ctgttcacca
    23101 tcgaccagcc gcgctatcaa aaggcgcttg aggaagcgca agccgatgtt gcttattatc
    23161 aggtactggc acaggagaaa cgccaggagg ccggacgtcg taaccgtctc ggtgtgcagg
    23221 cgatgtctcg cgaagagatc gaccaggcta acaacgtact acaaacggtt ctgcatcagt
    23281 tagcgaaagc gcaggcgacc cgcgatctgg caaaactgga tcttgaacgc acggtgatcc
    23341 gagcgccagc agatggctgg gtgaccaacc tcaacgtcta taccggtgag tttattactc
    23401 gaggatcaac ggcggttgcg ctggtgaaac agaactcctt ctatgtactg gcctatatgg
    23461 aagaaactaa gctggaaggg gtgcgtccgg ggtatcgtgc agagatcacg ccgcttggca
    23521 gtaacaaagt gctgaaaggg actgttgata gtgttgccgc tggggtcacc aacgccagca
    23581 gcacgcgtga cgacaaaggg atggcgacca tcgactctga ccttgaatgg gtgcgtttgg
    23641 cgcaacgtgt tccggttcgt attcgtctcg acaaccagca agagaacatc tggcctgcgg
    23701 gcaccactgc tacagtggtg gtcactggca aacaagatcg cgacgaaagc caggattcgt
    23761 tcttccgtaa aatggcccat cgcctgcgtg agtttggtta atcacgatgg gtattttctc
    23821 cattgctaac caacatattc gctttgcggt aaaactggcg accgccattg tactggcgct
    23881 gtttgttggc tttcacttcc agctggaaac gccacgctgg gcggtactga cagcggcgat
    23941 tgttgccgcc ggtccggcct ttgctgcggg aggtgaaccg tattctggcg ctattcgcta
    24001 tcgtggcttt ttgcgcatca tcggcacatt tattggctgt attgccggac tggtgatcat
    24061 cattgcgatg atccgcgcac cattattgat gattctggtg tgctgtatct gggccggttt
    24121 ttgtacctgg atatcctcgc tggtacgaat agaaaactcg tacgcgtggg ggctggccgg
    24181 ttataccgcg ctgatcattg tgatcaccat tcagccggaa ccattgctta cgccgcagtt
    24241 tgccgtcgaa cgttgtagcg agatcgttat cggtattgtg tgtgcgatta tggcggattt
    24301 gctcttttct ccgcgatcga tcaaacaaga agtagatcga gagctggaaa gtttgctggt
    24361 cgcgcaatat caattaatgc aactctgtat caagcatggc gatggtgaag ttgtcgataa
    24421 agcctggggc gacctggtgc gacgcaccac ggcactacaa ggcatgcgca gcaacctgaa
    24481 tatggaatct tcccgctggg cgcgggccaa tcgacgttta aaagcgatca atacgctatc
    24541 gctgacgctg attacccaat cctgcgaaac ttatcttatt cagaatacgc gcccggaatt
    24601 gatcactgat actttccgcg aattttttga cacgccggta gaaaccgcgc aggacgtcca
    24661 caagcagctc aaacgcctgc ggagagttat cgcctggact ggggaacggg aaacgcctgt
    24721 caccatttat agctgggtcg cggcggcaac gcgttatcag cttctcaagc gcggcgttat
    24781 cagtaacaca aaaatcaacg ccaccgaaga agagatcctg caaggcgaac cggaagtaaa
    24841 agtagagtca gccgaacgtc atcatgcaat ggttaacttc tggcgaacca cactttcctg
    24901 cattctgggc acgcttttct ggctgtggac gggctggact tccggcagtg gtgcaatggt
    24961 gatgattgcg gtcgtgacgt cactggcaat gcgtttgccg aatccacgca tggtggcgat
    25021 cgactttatc tatgggacgc tggccgcgct gccgttaggg ctgctctact ttttggtgat
    25081 tatccctaat acccaacaga gcatgttgct gctgtgtatt agcctggcag tgctgggatt
    25141 cttcctcggt atagaagtac agaaacggcg actgggctcg atgggggcac tggccagcac
    25201 cataaatatt atcgtgctgg ataacccgat gactttccat ttcagtcagt ttctcgacag
    25261 cgcattagga caaatcgtcg gctgtgtgct cgcgttcacc gttattttgc tggtgcggga
    25321 taaatcgcgc gacaggactg gacgtgtact gcttaatcag tttgtttctg ccgctgtttc
    25381 cgcgatgact accaatgtgg cacgtcgtaa agagaaccac ctcccggcac tttatcagca
    25441 gctgtttttg ctgatgaata agttcccagg ggatttgccg aaatttcgcc tggcgctgac
    25501 gatgattatc gcgcaccagc gcctgcgtga tgcgccgatc ccggttaacg aggatttatc
    25561 ggcgtttcac cgacaaatgc gccgcacagc agaccatgtg atatctgccc gtagcgatga
    25621 taaacgtcgt cggtactttg gccagttgct ggaagaactg gaaatctacc aggaaaagct
    25681 acgcatctgg caagcgccac cgcaggtgac ggaaccggtt catcggctgg cggggatgct
    25741 ccataagtat caacatgcgt tgaccgatag ttaagtcaaa accgacgcca aaagcgtcgg
    25801 ttttttcatg gctatactta gcgatgcacg gcagaactcg ccgcgaaacg tgacggtggc
    25861 aacagatgaa tatttatgcc tttgattttg atgagattga gagtcaggag gatttttatc
    25921 gtgactttag ccaaaccttt ggtctggcta aagataaggt acgcgatctc gactcactat
    25981 gggatgtgtt aatgaacgat gtcctgccgc taccacttga gattgaattt gttcatctgg
    26041 gagagaaaac gcgtcgccgt tttggcgcgt taatattgct gtttgatgag gcagaggaag
    26101 agctggaagg gcatttgcgt tttaatgttc gtcattagcg caaaaaaaag cccacgaacc
    26161 gggggcaata tcgtcggaca aaacgatgag ggtttatttg tacagttcag ccgtagcgtg
    26221 ccaggtgtca ccgctacgag cttcagtaat ctggtaggcc gttgcgcctt tctcttccgc
    26281 ttttttgttc agcatttcac gtatatccat tggcgaagac gccacaccac ttacggatac
    26341 ggtcccgatt gcttcacgat tttgtgcttg tgcagcatca atggagtcgg cagcgaatgc
    26401 accgaaagag agaacagaaa gtacgcttaa tgcagctaca gtggttttga ttttcatgat
    26461 ttttacctcg acataatctt ttagctgggc ctttgtttcg tgacccatat cacaaaatca
    26521 agtatacact aattattgaa ctaattaata ccacactaac tgtttcagtt gatactaagt
    26581 gttaaaaatg tgaattttaa taacaaaaaa gtataaaaaa cacggttatt tattattaat
    26641 tatagtattt tcaattagat aatgaatttt gcataatgtg ctttttttac cgtatgtatt
    26701 cattttgtga atgatgtgtc gcggtaaaac gcactattcg ttaaatgtat cgcaggagaa
    26761 agaaggggaa tgagagggat aagcaacatt ttccccgccg ccagaagcga cggggcagag
    26821 attaaagctc ctggtcgaac agctctaaaa tcgcttcgta caggtctttg acggtgaaac
    26881 cgttagcagg ggtggtaaag atggtgtcat cgccagcgat ggtgcccaga ataccttctg
    26941 ctttgcccag tgagtccagc aggcgagcaa ttaactgcgc cgcgccaggg ctggtatgaa
    27001 tcacgacaac tgcatcgttg tagtcgatat ccagcaccag attcttcaat ggactggagg
    27061 tggttggtac acccagttca gctggcaggc agtaaaccat ttccattttg gcattgcgtg
    27121 tacgtacagc accaaacttg gtcaacatcc gcgagacttt agactgatta atattgtcaa
    27181 agccttgctc ctgcaacgcg gcgacgattt cgccctggga gctaaatttc tcttctttaa
    27241 gtaatgcttt aaatgcttta actagttctt cttgcttagc cgagcttcgc ataagtcacc
    27301 cgatatggtg gttgatacaa cattattgtg catacagatg aatttttatg caaacagtca
    27361 gccctgaaga aggctgaaat aatgttatga aagagcggga ttttatcaaa tttcgttatt
    27421 gagaaacatg cctgcgtcac ggcatgcaaa ttctgtttaa aagtaaatta attgttatca
    27481 aattgatgtt gttttggctg aacggtaggg tatattgtca ccacctgttg gaatgttgcg
    27541 ctaatgcata agcgactgtt aattacgtaa gttaggttcc tgattacggc aattaaatgc
    27601 ataaacgcta aacttgcgtg actacacatt cttgagatgt ggtcattgta aacggcaatt
    27661 ttgtggatta aggtcgcggc agcggagcaa catatcttag tttatcaata taataaggag
    27721 tttaggatga aagtcgcagt cctcggcgct gctggcggta ttggccaggc gcttgcacta
    27781 ctgttaaaaa cccaactgcc ttcaggttca gaactctctc tgtatgatat cgctccagtg
    27841 actcccggtg tggccgtcga tctgagccat atccctactg ctgtgaaaat caaaggtttt
    27901 tctggtgaag atgcgactcc ggcgctggaa ggcgcagatg tcgttcttat ctctgcaggc
    27961 gtagcgcgta aaccgggtat ggatcgttcc gacctgttta acgttaacgc cggcatcgtg
    28021 aaaaacctgg tacagcaagt tgcgaaaaac tgcccgaaag cgtgcattgg tattatcact
    28081 aacccggtta acaccacagt tgcaattgct gctgaagtgc tgaaaaaagc cggtgtttat
    28141 gacaaaaaca aactgttcgg cgttaccacg ctggatatca ttcgttccaa cacctttgtt
    28201 gcggaactga aaggcaaaca gccaggcgaa gttgaagtgc cggttattgg cggtcactct
    28261 ggtgttacca ttctgccgct gctgtcacag gttcctggcg ttagttttac cgagcaggaa
    28321 gtggctgatc tgaccaaacg tatccagaac gcgggtactg aggtggttga agcgaaagcc
    28381 ggtggcgggt ctgcaaccct gtctatgggc caggcagctg cacgttttgg tctgtctctg
    28441 gttcgtgcac tgcagggcga acaaggcgtt gtcgaatgtg cctacgttga aggcgacggt
    28501 cagtacgccc gtttcttctc tcaaccgctg ctgctgggta aaaacggcgt ggaagagcgt
    28561 aaatctatcg gtaccctgag cgcatttgaa cagaacgcgc tggaaggtat gctggatacg
    28621 ctcaagaaag atatcgccct gggcaaagag ttcgttaata agtaattgat tagcgaataa
    28681 taaaaaaccg gagcacagac tccggttttt gttttgagca ctcgacttaa ttggttgccg
    28741 gatattcctg aatggtgacc tgcagcgtta actgcttatc atcacgcatc actacaaccg
    28801 ggatcaccga accagggcga atttccgcca cctgatccat cgtctccaga gcagagatgg
    28861 ccggtttgtt atccaccgaa ataatcagat cgttgacctg aatacccgca ttcgccgctg
    28921 ggccgtcagg tgacacttca ttaaccacga tcccttgcag ttgatctata ccaccgctct
    28981 gcgcgtgcag tggtgcgatc tcgcgtccgc cgataccaat gtagccgcgg atcacgcgac
    29041 catcgcggat cagcttatcc ataattttgg ttgctaactg gaaaggaatc gcaaagccga
    29101 taccttccgg cgtttcgcca tcgttactct tatcaaacga cagcgtgtta atgcccatca
    29161 gttctcccag cgagttcacc agcgcgccgc cagagttacc gtggttaatg gaagcatcgg
    29221 tttgtaggaa gttttgccgc ccggtcgggt tcagaccgat tcgacccgtg gcactaataa
    29281 tcccttgggt aatggtctgc ccgaggttgt acgggttacc gatcgccagt actacgtcgc
    29341 caatgtgcgg tacgcgacgt gcattaattg gaatggtagg taaaccgcca gtggcattaa
    29401 ttttaagtac cgccagatcg gttagagagt cagatcccac cagcaatgct tcaaatacac
    29461 gtccatcctg taaggcgacg atgatctgat cggcgtcgtt gatgacgtgt ttattggtga
    29521 tgatataacc gcgttgatcc atgattacac cggatcccag ggtgcggatc tcaagctggt
    29581 tgtgagagtt ggtgttcaaa ccacggttgt aaacgttaac caccgctggc gcggcgcggc
    29641 gaaccgccag attatagctg gcaggcgtct catcggtact gtcaaattgc ggagtggaaa
    29701 gcgggttaag gctgcgcagc gaaggcatgg caaccagcag aatagcgccg acaattaatc
    29761 caatcgcaac ggaacgtaag agcttcacaa acatgatgga ggcgtcatta aaaaagggaa
    29821 cggcagcagc ataccacgag ttaaccggac atcacacgtc agcctgatgc ccggtttacg
    29881 acattaacgc atcagcagat agatgctttc attgccgcgt acaatttgca gggcgatgat
    29941 ggccggtttt gccgccagca ctttacgcat ttcagcaatc gagttcaccc gatcgcggtt
    30001 gacgccaatg atcacatcgt ctttttgcaa gccagcctga gcagctgggc ttcctttgac
    30061 aacttcatct atcttaatac ctttgccgcc atcttttagc tgaccatcgc tcaacgttgc
    30121 accttccagc gctggccgac gaagaggtgc tggtatcaag cgtcacttct acttccagtg
    30181 gtttgccgtt acgcagcagg ccaagcttca ctttcgtgcc cggctctgta gtcgcgatac
    30241 gagagcgcaa ctcagcaaag ctattcagcg gtttgccgtt gaggctggta ataatatcgc
    30301 ccgctttgac gcccgctttc gctgagccag aacttggcaa cacttcgctg acaaacgcgc
    30361 cacgctgcac gtcaaggttg aaggctttgg cgatatcggc actcatctcg gtgcctttga
    30421 tgcctaacaa accgcgtttg atttcaccaa agtcgataag ctgctgcgcc agtgttcgcg
    30481 ccatattact ggggatggca aatccaatcc cgacgctccc gccgccaggc gcaaggattg
    30541 cagtgttgat gccaattaac tcaccgttaa ggtttaatag tgcaccgccg gagttaccgc
    30601 ggttaatgga agcatctgtc tggataaagt tttccagacc ttcaagattc aacccgctgc
    30661 ggcctaatgc ggaaacaatg ccagaggtgg cggtttgccc aaggccaaat gggttaccga
    30721 ccgctacggc aaaatcaccg acgcgcaatt tatcggagtc ggcaatagcg atttgcgtta
    30781 atttgctcgg gttttgaatt tgtaacaggg cgatatcgct ctggtcatcg ctaccaatca
    30841 gttttgcatc aaactcgcgc ccatcattga gctgaatact gattttctgt gcctgattaa
    30901 tcacatggtt gttggtcagc acatagcctt tactggcgtt gatgatgaca ccggaaccta
    30961 aaccttcgaa gggttgtgca ggttgatccg gtaaatcatc accaaaaaac tttttgaatt
    31021 cttccgggat tttctgtccc tgactggccg ttccttccac ccgtacgctc accactgccg
    31081 gaagcacttt ttccagcatt ggagccagac tggggagagg ggcctgatcg gcaacctggc
    31141 ctggaatcga cgcgacggcc tgaaatgacg ccgagagagt taacccggca cttaacgcta
    31201 atgcactcaa cagctgggtt tgttttttca ttattcctgc tctcgtacct gaatgataag
    31261 aaaagagatt caaaacagtt tgatgttatt gaattttcag acggctaaca atgagacacc
    31321 agattaaatg atagtcgggg agggagagaa gaggagggcg caatggctgc gcccgaaaaa
    31381 taaattagtc gcgcttcgcg ccagtacgca gcaggccgga tgcgccttca gaatagtcgc
    31441 gaggcacctg caccggtgcc tgatcgttgc tggcttcaga ctctgccaga cgattacgga
    31501 acgggtttgc ttcagcagac agttccggca gcaggctgct ggagcttttt gccatgtgct
    31561 gatacagctg gcgatagtcg tgcgccatgg tatccagtaa ttccgcgctg cgggcaaagt
    31621 ggctaaccag ctcttcgcga tactcgtcca gttcagcttt attcttttcc agttcgtact
    31681 gcaacgcctg ttgctggcgt agtttacgat taccaaaacg catggccaca gcaccaataa
    31741 tgatgccgac gactaaccca attagcgcat attcccaggt catgaacatc tcccgttgtc
    31801 ttttgtttcc gtagggtgtt ggcttcaggc tccatgcctg cggctgatta tgccactata
    31861 accgttaatt ccacagaagt ggaatcccga ctgcatatcg cgtagtgtag aacggccttt
    31921 ttttcgtcaa ccgtgaacaa cggcgcaccg attattcaag gaataacaat aagatcatgc
    31981 aaagcgttac cccaacatcg caatacctga aggcgctcaa tgaaggcagc catcaacccg
    32041 acgacgttca aaaagaggcc gtcagccgcc tggaaattat ttatcaagaa ctcatcaata
    32101 gcaggccacc agcccccagg acgagtgggc taatggcgcg ggtcggtaag ctgtggggta
    32161 aacgcgaaga cacaaagcat acgccagtgc gtggcttata tatgtggggc ggtgtaggac
    32221 gcgggaaaac ctggctgatg gaccttttct atcaaagcct gccgggagag cggaaacagc
    32281 gcctgcactt tcaccgtttt atgctgcggg tgcacgaaga gctaactgcc ttacaggggc
    32341 agaccgatcc gctggaaatt attgccgatc gctttaaagc cgaaactgac gtgctctgtt
    32401 ttgacgaatt ttttgtttct gatattaccg acgccatgct acttggcggt ctgatgaaag
    32461 ccctgttcac ccgcggtatt accctggtag cgacgtcaaa tattccgccg gacgaacttt
    32521 atcgaaatgg cctgcaacgt gcgcgttttc tgcctgcaat cgatgccatt aaacagcatt
    32581 gtgatgtaat gaacgtggac gctggtgttg attatcgact gcgtacactc actcaggcgc
    32641 atctgtggct ttcgccactc aacgatgaaa cccgggcgca gatggataaa ctatggttgg
    32701 cgctggcggg ggtgaaacga gaaaattcac cgacgttaga aatcaaccat cggccattgg
    32761 cgacaatggg cgtcgagaac cagacgctgg cggtttcttt tactacgctg tgcgtcgacg
    32821 cccgcagtca gcatgactat attgcgctct cacgcctgtt tcacacagta atgttgtttg
    32881 atgtgccagt catgacgcgg ttgatggaga gcgaagcgcg gcgctttatt gcgctggtgg
    32941 atgagtttta cgagcgccat gtcaaattag tggtgagtgc agaagtgccg ctgtatgaaa
    33001 tttatcaggg tgagcggctg aagtttgagt tccagcgttg cctgtcacgt ctgcaagaga
    33061 tgcaaagcga agagtatctg aagcgcgagc atttggcggg ttaaaacctg ccacaaatca
    33121 caaaaagggg tcgatctttg accccgactt ctctataatc ctgcgacccc acgttacaag
    33181 aaagtttttt tcccaaaact ttttgtgtgc tggcataggc tattcgaagg ggtaggtttg
    33241 ccggactttg tcgtgtgaac ctcaacaatt gaagacgttt gggtgttcac caacgtgtaa
    33301 ctatttattg ggtaagcttt taatgaaaac ttttacagct aaaccagaaa ccgtaaaacg
    33361 cgactggtat gttgttgacg cgaccggtaa aactctgggc cgtctggcta ctgaactggc
    33421 tcgtcgcctg cgcggtaagc acaaagcgga atatactccg cacgtagata ccggtgatta
    33481 catcatcgtt ctgaacgctg acaaagttgc tgtaaccggc aacaagcgta ctgacaaagt
    33541 gtactatcac cacaccggcc acatcggtgg tatcaaacaa gcgacctttg aagagatgat
    33601 tgctcgccgt cctgagcgtg tgattgaaat cgcggttaaa ggcatgttgc caaaaggccc
    33661 gctgggtcgt gctatgttcc gtaaactgaa agtttacgcg ggtaacgagc acaaccacgc
    33721 ggcacagcaa ccgcaagttc ttgacatcta atcgggatta taggcaatgg ctgaaaatca
    33781 atactacggc actggtcgcc gcaaaagttc cgcagctcgc gttttcatca aaccgggcaa
    33841 cggtaaaatc gtaatcaacc aacgttctct ggaacagtac ttcggtcgtg aaactgcccg
    33901 catggtagtt cgtcagccgc tggaactggt cgacatggtt gagaaactgg acctgtacat
    33961 caccgttaaa ggtggtggta tctctggtca ggctggtgcg atccgtcacg gtatcacccg
    34021 cgctctgatg gaatacgacg agtccctgcg ttctgaactg cgtaaagctg gcttcgttac
    34081 tcgtgacgct cgtcaggttg aacgtaagaa agtcggtctg cgtaaagcac gtcgtcgtcc
    34141 gcagttctcc aaacgttaat tggcttctgc tacggcagaa aacaatttgc gaaaaaaccc
    34201 gcttcggcgg gtttttttat ggctaaattc tgaatcagcg taaaaaatgg gaagttgctt
    34261 tttgctgccg tctgaaagac aggtaaaaca aaaaccacat cgccaataag ggtctaagtc
    34321 aactatttca gactaaagcg catctctttt tccccatttc cggcatccac tcaccacaaa
    34381 ggtcgcaaaa tctggtaaac tatcatccaa ttttctgccc aaatgtcggg tattgctcat
    34441 tttttgtttg attttcgaac aaagagagtc gttccttatt gggtaacaca acttctgact
    34501 ggccacctgg tggctggtag cagtaaaaat tctgactata cctggaggtt ttcatggctg
    34561 tcgctgccaa caaacgttcg gtaatgacgc tgttttccgg tcctactgac atctatagcc
    34621 atcaggtccg cattgtgctg gctgagaaag gtgtaagttt cgagatcgaa cacgtggaaa
    34681 aggacaatcc gcctcaggat ctgattgacc tcaacccgaa tcagagcgtt ccgaccctgg
    34741 tggatcgtga gctgaccctg tgggaatctc gcatcattat ggaatatctg gatgagcgtt
    34801 tcccgcatcc gccactgatg cctgtttacc cggtagctcg cggtgaaagc cgtctgtaca
    34861 tgcatcgcat cgaaaaagac tggtacacgc tgatgaacac tatcatcaac ggttcagctt
    34921 ctgaagcaga tgccgcacgt aagcaactgc gcgaagaact gctggcgatt gcgccggtct
    34981 tcggtcagaa gccgtacttc ctgagcgatg agttcagcct ggtcgattgc taccttgctc
    35041 cgctgctgtg gcgtctgccg caactgggca tcgagttcag cggcccgggt gcgaaagagc
    35101 tgaaaggcta tatgacccgc gtctttgagc gtgactcttt ccttgcttct ttaactgaag
    35161 cagaacgtga aatgcgtctg ggccggagtt aatctgtatg gatttgtcac agctaacacc
    35221 acgtcgtccc tatctgctgc gtgcattcta tgagtggttg ctggataacc agctcacgcc
    35281 gcacctggtg gtggatgtga cgctccctgg cgtgcaggtt cctatggaat atgcgcgtga
    35341 cgggcaaatc gtactcaaca ttgcgccgcg tgctgtcggc aatctggaac tggcgaatga
    35401 tgaggtgcgc tttaacgcgc gctttggtgg cattccgcgt caggtttctg tgccgctggc
    35461 tgccgtgctg gctatctacg cccgtgaaaa tggcgcaggc acgatgtttg agcctgaagc
    35521 tgcctacgat gaagatacca gcatcatgaa tgatgaagag gcatcggcag acaacgaaac
    35581 cgttatgtcg gttattgatg gcgacaagcc agatcacgat gatgacactc atcctgacga
    35641 tgaacctccg cagccaccac gcggtggtcg accggcatta cgcgttgtga agtaatacaa
    35701 aacaggccca ggcggcctgt tttgtctttt taatgactta ataaaatagc gtgattgtgg
    35761 caatcatatt caccacgaaa ccgactgcca tggggatcgc tgtccgcttc accaccgcaa
    35821 aaggcgaaac gcctgcaatt cctgacgtaa cgaccaccac cgcagtaatc ggcgaaaccg
    35881 cgcgcgctag cgtcgtggca aaatgcatcg gcataatcat tacaaccgct ggtacatgta
    35941 gtccggctgc gatattcgga ataagactgg caaatgacat aaacggcgca ttgccagagc
    36001 ccatcacaat ggcacaaatg gcaatgacta gcgccataat aatcatcacg ccaataccgc
    36061 ccagaccaga atgctccgca cccctgataa ccgcatcaac agtgccaatc gtcgttaagc
    36121 ctttcgcaaa tatttcaccc gcgaccacca gcgttaccac gttgacaaac tgcgtaccca
    36181 tgccgtcaaa aaacgcctgc acatcgtcca ttgtttcgcg caagttatgc ttgcggaaga
    36241 actcaacaaa catcgtcacc gtcaaactta gtaacatcac caccaccaga tgcagttctg
    36301 actgcatcag cccgacgtgg gcgaggaaca gcgagccgag catcaggatt aacggcatca
    36361 caggtaaaat ggcgtaatag agcggcggga cattatcgag agttttttgc tctgcctgtt
    36421 cgtgattgat atttttatct tttttgtcaa aagcgcgttg cacgaaaaag tgggagatcg
    36481 ccaccgagat aatgacgcaa gaggcgaccg gaagctggta gtggaagaag tatgtggcaa
    36541 ttttcattcc cgcgacctgg gctgcaaaaa tggagttcgt ttccagaatc ccccactcaa
    36601 tggacatcgt ggttgcgata accgccaccg cagagaggcg acttactccc agactcacca
    36661 gcgtcggaaa taaggtgacc atcagcaaca tacccagacc ggaggcgctg gtaataaact
    36721 gcgccatgat ttggccgatg acgtaagttg ccgacagaat aatatacggc gagcgaatga
    36781 gttttaacgg gcggcttagc aagctcacca tcgcgcaact ggctcctatg cgctccatgt
    36841 agcgggcata accgcccacc gccataatcg acagccccaa tccggcaatg cgg
//
LOCUS       DAJTRC010000031        36596 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.031, whole genome shotgun sequence.
ACCESSION   DAJTRC010000031 DAJTRC010000000
VERSION     DAJTRC010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 36596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.031"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(156..1391)
                     /locus_tag="OO357_001675"
     CDS             complement(156..1391)
                     /locus_tag="OO357_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709601.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5886655.1"
                     /translation="MLQQVPTRAFHVIAKPSGSDCNLNCDYCFYLEKQSLYREKPVTH
                     MDDDTLEAYIRHYIAASELQNEVAFTWQGGEPTLLGLEFYRRAVALQAKYGAGRKISN
                     SFQTNGVLLDDEWCAFLAENNFLVGLSLDGPAEIHNQYRVTKGGRPTHKLVMRALTLL
                     QKHHVDYNVLVCVNRTSAQQPLQVYDFLCDAGVEFIQFIPVVERLADETAVRDGLKLH
                     APGDIQGELTEWSVRPDEFGEFLVAIFDHWIKRDVGKIFVMNIEWAFANFVGAPGAVC
                     HHQPTCGRSVIVEHNGDVYACDHYVYPQYRLGNMHQQTIAEMIDSPQQQVFGEDKFKQ
                     LPAQCRSCNVLKACWGGCPKHRFMLDASGKPGLNYLCAGYQRYFRHLPPYLKAMADLL
                     AHGRPASDIMQAHLMVVNK"
     gene            complement(1538..1614)
                     /locus_tag="OO357_001676"
     tRNA            complement(1538..1614)
                     /locus_tag="OO357_001676"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1578..1580),aa:Pro,seq:tgg)
     gene            complement(1657..1743)
                     /locus_tag="OO357_001677"
     tRNA            complement(1657..1743)
                     /locus_tag="OO357_001677"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1707..1709),aa:Leu,seq:cag)
     gene            complement(1764..1839)
                     /locus_tag="OO357_001678"
     tRNA            complement(1764..1839)
                     /locus_tag="OO357_001678"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1804..1806),aa:His,seq:gtg)
     gene            complement(1898..1974)
                     /locus_tag="OO357_001679"
     tRNA            complement(1898..1974)
                     /locus_tag="OO357_001679"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1938..1940),aa:Arg,seq:ccg)
     gene            complement(2077..3462)
                     /locus_tag="OO357_001680"
     CDS             complement(2077..3462)
                     /locus_tag="OO357_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5886656.1"
                     /translation="MADNKPELQRGLEARHIELIALGGTIGVGLFMGAASTLKWAGPS
                     VLLAYIIAGLFVFFIMRSMGEMLFLEPVTGSFAVYAHRYMSPFFGYLTAWSYWFMWMA
                     VGISEITAIGVYVQFWSPEMAQWIPALIAVALVALANLAAVRLYGEIEFWFAMIKVTT
                     IIVMIVIGLGVIFFGFGNGGQSIGFSNLTEHGGFFAGGWKGFLTALCIVVASYQGVEL
                     IGITAGEAKNPQVTLRSAVGKVLWRILIFYVGAIFVIVTIFPWNEIGSNGSPFVLTFA
                     KIGITAAAGIINFVVLTAALSGCNSGMYSCGRMLYALAKNRQLPAAMAKVSRHGVPVA
                     GVAVSIAILLIGSCLNYIIPNPQRVFVYVYSASVLPGMVPWFVILISQLRFRRAHKAA
                     IASHPFRSILFPWANYVTMAFLICVLIGMYFNEDTRMSLFVGIIFMLAVTAIYKVFGL
                     NRHGKAHKLEE"
     gene            complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="OO357_001681"
     CDS             complement(3653..4393)
                     /gene="rffM"
                     /locus_tag="OO357_001681"
                     /EC_number="2.4.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylmannosaminouronosyltransferase"
                     /protein_id="HCR5886657.1"
                     /translation="MNNNTTAPTYTLRGLQLIGWRDMQHALDYLFADGQLKQGTLVAI
                     NAEKMLTIEDNAEVRELINAAEFKYADGISIVRSVRKKYPQAQVSRVAGADLWEELMA
                     RAGKEGTPVFLVGGKPEVLAQTEAKLRNQWNVNIVGSQDGYFKPEQRQALFERIHASG
                     AQIVTVAMGSPKQEIFMRDCRLVHPDALYMGVGGTYDVFTGHVKRAPKIWQTLGLEWL
                     YRLLSQPSRIKRQLRLLRYLRWHYTGNL"
     gene            complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="OO357_001682"
     CDS             complement(4396..5748)
                     /gene="wzyE"
                     /locus_tag="OO357_001682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA oligosaccharide polymerase"
                     /protein_id="HCR5886658.1"
                     /translation="MSLLQFSGLFVVWLLCTLFIATLTWFEFRRVRFNFNVFFSLLFL
                     LTFFFGFPLTSVLVFRFDVGVAPPEILLQVLLSAGCFYAVYYVTYKTRLRKRVADVPR
                     RPLFTMNRVETNLTWVILMGIALVSVGIFFMHNGFLLFRLNSYSQIFSSEVSGVALKR
                     FFYFFIPAMLVVYFLRQDSKAWLFFLVSTVAFGLLTYMIVGGTRANIIIAFAIFLFIG
                     IIRGWISLWMLAAAGVLGIVGMFWLALKRYGMNVSGDEAFYTFLYLTRDTFSPWENLA
                     LLLQNYDNIDFQGLAPIVRDFYVFIPSWLWPGRPSMVLNSANYFTWEVLNNHSGLAIS
                     PTLIGSLVVMGGALFIPLGAIVVGLIIKWFDWLYELGNRETNRYKAAILHSFCFGAIF
                     NMIVLAREGLDSFVSRVVFFIVVFGACLMIAKLLYWLFESAGLIHKRTKSSLRTQVEG
                     "
     gene            complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="OO357_001683"
                     /gene_synonym="wecF"
     CDS             complement(5745..6824)
                     /gene="rffT"
                     /locus_tag="OO357_001683"
                     /gene_synonym="wecF"
                     /EC_number="2.4.1.325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDP-N-acetylfucosamine:lipid II
                     N-acetylfucosaminyltransferase"
                     /protein_id="HCR5886659.1"
                     /translation="MTVLIHVLGSDIPHHNRTVLRFFNDALAATSEHAREFMVVGKDD
                     GLSDSCPALSVQFFPGKKSLAEAVIAKAKANRQQRFFFHGQFNPTLWLALLSGGIKPS
                     QFFWHIWGADLYELSSGLRYKLFYPLRRLAQKRVGCVFATRGDLSFFAKTHPKVRGEL
                     LYFPTRMDPSLNTMANDRQREGKMTILVGNSGDRSNEHVAALRAVHQQFGDTVKVVVP
                     MGYPPNNEAYIEEVRQAGLELFSEENLQILSEKLEFDAYLALLRQCDLGYFIFARQQG
                     IGTLCLLIQAGIPCVLNRENPFWQDMTEQHLPVLFTTDDLNEDIVREAQRQLASADKN
                     TIAFFSPNYLQGWQRALAIAAGEVA"
     gene            complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="OO357_001684"
     CDS             complement(6821..8071)
                     /gene="wzxE"
                     /locus_tag="OO357_001684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid III flippase WzxE"
                     /protein_id="HCR5886660.1"
                     /translation="MSLAKASLWTAASTLVKIGAGLLVGKLLAVSFGPAGLGLAANFR
                     QLITVLGVLAGAGIFNGVTKYVAQYHDNPQQLRRVVGTSSAMVLGFSTLMALVFVLAA
                     APISQGLFGNTDYQGLVRLVALVQMGIAWGNLLLALMKGFRDAAGNALSLIVGSLIGV
                     LAYYVSYRLGGYEGALLGLALIPALVVIPAAIMLIKRGVIPLSYLKPSWDNGLAGQLS
                     KFTLMALITSVTLPVAYIMMRKLLAAQYSWDEVGIWQGVSSISDAYLQFITASFSVYL
                     LPTLSRLTEKRDITREVVKSLKFVLPAVAAASFTVWLLRDFAIWLLLSNKFTAMRDLF
                     AWQLVGDVLKVGAYVFGYLVIAKASLRFYILAEVSQFTLLMVFAHWLIPAHGALGAAQ
                     AYMATYIVYFSLCCGVFLLWRRRA"
     gene            complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="OO357_001685"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
     CDS             complement(8073..9203)
                     /gene="rffA"
                     /locus_tag="OO357_001685"
                     /gene_synonym="fcnA"
                     /gene_synonym="wecE"
                     /EC_number="2.6.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxygalactose transaminase"
                     /protein_id="HCR5886661.1"
                     /translation="MIPFNAPPVVGTELDYMQSAMGSGKLCGDGGFTRRCQQWLEQRF
                     GSAKVLLTPSCTASLEMAALLLDIQPGDEVIMPSYTFVSTANAFVLRGAKIVFVDVRP
                     DTMNIDETLIEAAITDKTRVIVPVHYAGVACEMDTIMALAKKHNLFVVEDAAQGVMST
                     YKGRALGTIGHIGCFSFHETKNYTAGGEGGATLINDKALIERAEIIREKGTNRSQFFR
                     GQVDKYTWRDIGSSYLMSDLQAAYLWAQLEAADRINQQRLALWQNYYDTLAPLAKAGR
                     IELPSIPDGCVQNAHMFYIKLRDIDDRSALINFLKEAEIMAVFHYIPLHGCPAGEHFG
                     EFHGEDRYTTKESERLLRLPLFYNLSPVNQRTVIATLLNYFS"
     gene            complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="OO357_001686"
                     /gene_synonym="wecD"
     CDS             complement(9208..9882)
                     /gene="rffC"
                     /locus_tag="OO357_001686"
                     /gene_synonym="wecD"
                     /EC_number="2.3.1.210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026256.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-amino-4,6-dideoxy-D-galactose
                     acyltransferase"
                     /protein_id="HCR5886662.1"
                     /translation="MPVRASIEPLTWENAFFGVNSAIVRITSEAPLLTSDALAPWSRV
                     QAKIAASNTGELDALQQLGFSLVEGEVDLALPVNNVSDSGAVVAQETDIPALRQLASA
                     AFAQSRFRAPWYAPDASGRFYAQWIENAVRGTFDHQCLILRAASGDIRGYVSLRELNA
                     TDARIGLLAGRGAGAELMQTALNWAYARGKTTLRVATQMGNTAALKRYIQSGANVEST
                     AYWLYR"
     gene            complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="OO357_001687"
     CDS             complement(9860..10741)
                     /gene="rfbA"
                     /locus_tag="OO357_001687"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HCR5886663.1"
                     /translation="MKGIILAGGSGTRLHPITRGVSKQLLPIYDKPMIYYPLSVLMLA
                     GIREILIITTPEDKGYFQRLLGDGSEFGIQLEYAEQPSPDGLAQAFIIGETFLNGEPS
                     CLVLGDNIFFGQGFSPKLRHVAARTEGATVFGYQVMDPERFGVVEFDDNFRAISLEEK
                     PKQPKSNWAVTGLYFYDSKVVEYAKQVKPSERGELEITSINQMYLEAGNLTVELLGRG
                     FAWLDTGTHDSLIEASTFVQTVEKRQGFKIACLEEIAWRNGWLDDEGVKRAASSLAKT
                     GYGQYLLELLRARPRQY"
     gene            complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="OO357_001688"
     CDS             complement(10760..11827)
                     /gene="rffG"
                     /locus_tag="OO357_001688"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HCR5886664.1"
                     /translation="MRKILITGGAGFIGSALVRYIINETSDAVVVVDKLTYAGNLMSL
                     APVAQSERFAFEKVDICDRAELARVFTEHQPDCVMHLAAESHVDRSIDGPAAFIETNI
                     VGTYTLLEAARAYWNTLTEDKKSAFRFHHISTDEVYGDLHSTDDFFTETTPYAPSSPY
                     SASKASSDHLVRAWLRTYGLPTLITNCSNNYGPYHFPEKLIPLMILNALAGKPLPVYG
                     NGQQIRDWLYVEDHARALYCVATTGKVGETYNIGGHNERKNLDVVETICELLEELASN
                     KPHGVAYYRDLITFVADRPGHDLRYAIDASKIARELGWLPQETFESGMRKTVQWYLAN
                     ESWWKQVQDGSYQGERLGLKG"
     gene            complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="OO357_001689"
     CDS             complement(11827..13089)
                     /gene="wecC"
                     /locus_tag="OO357_001689"
                     /EC_number="1.1.1.336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetyl-D-mannosamine dehydrogenase"
                     /protein_id="HCR5886665.1"
                     /translation="MSFATISVIGLGYIGLPTAAAFASRQKQVIGVDINQHAVDTINR
                     GEIHIVEPDLASVVKTAVEGGFLRASTTPVEADAWLIAVPTPFKGDHEPDMTYVESAA
                     RSIAPVLKKGALVILESTSPVGSTEKMAEWLAEMRPDLTFPQQVGEQADVNIAYCPER
                     VLPGQVMVELIKNDRVIGGMTPVCSARASELYKIFLEGECVVTNSRTAEMCKLTENSF
                     RDVNIAFANELSLICADQGINVWELIRLANRHPRVNILQPGPGVGGHCIAVDPWFIVA
                     QNPQQARLIRTAREVNDHKPFWVIDQVKAAVADCLAATDKRASELKIACFGLAFKPNI
                     DDLRESPAMEIAELIAQWHSGETLVVEPNIHQLPKKLTGLCTLAQLDEALATADVLVM
                     LVDHSQFKVINGDNVHQQYVVDAKGVWR"
     gene            complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="OO357_001690"
     CDS             complement(13086..14216)
                     /gene="wecB"
                     /locus_tag="OO357_001690"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HCR5886666.1"
                     /translation="MKVLTVFGTRPEAIKMAPLVHALAKDPFFEAKVCVTAQHREMLD
                     QVLKLFSIVPDYDLNIMQPGQGLTEITCRILEGLKPILAEFKPDVVLVHGDTTTTLAT
                     SLAAFYQRIPVGHVEAGLRTGALYSPWPEEANRTLTGHLAMYHFSPTETSRQNLLREN
                     VADSRIFITGNTVIDALLWVRDQVMSSDTLRSELAANYPFIAPDKKMILVTGHRRESF
                     GRGFEEICHALADIATTHQDIQIVYPVHLNPNVREPVNRILGHVKNVILIDPQEYLPF
                     VWLMNHAWLILTDSGGIQEEAPSLGKPVLVMRDTTERPEAVTAGTVRLVGTDKQRIVE
                     EVTRLLKDENEYQAMSRAHNPYGDGQACSRILEALKNNRISL"
     gene            complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="OO357_001691"
     CDS             complement(14272..15318)
                     /gene="wzzE"
                     /locus_tag="OO357_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECA polysaccharide chain length modulation
                     protein"
                     /protein_id="HCR5886667.1"
                     /translation="MTQPMPGKPAEDAENELDIRGLFRTLWAGKLWIIGMGLAFALIA
                     LAYTFFARQEWSSTAITDRPTVNMLGGYYSQQQFLRNLDVRSNMASADQPSVMDEAYK
                     EFVMQLASWDTRREFWLQTDYYKQRMVGNSKADAALLDEMINNIQFIPGDFTRAVNDS
                     VKLIAETAPDANNLLRQYVAFASQRAASHLNDELKGAWAARTIQMKAQVKRQEEVAKA
                     IYDRRMNSIEQALKIAEQHNISRSATDVPAEELPDSEMFLLGRPMLQARLENLQAVGP
                     AFDLDYDQNRAMLNTLNVGPTLDPRFQTYRYLRTPEEPVKRDSPRRAFLMIMWGIVGG
                     LIGAGVALTRRCSK"
     gene            complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="OO357_001692"
     CDS             complement(15330..16433)
                     /gene="wecA"
                     /locus_tag="OO357_001692"
                     /EC_number="2.7.8.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--undecaprenyl-phosphate
                     N-acetylglucosaminephosphotransferase"
                     /protein_id="HCR5886668.1"
                     /translation="MNLLTVSTDLISIFLFTTLFLFFARKVAKKVGLVDKPNFRKRHQ
                     GLIPLVGGISVYAGICFTFGIVDYYIPHASLYLACAGVLVFIGALDDRFDISVKIRAT
                     IQAAVGIVMMVFGKLYLSSLGYIFGSWEMVLGPFGYFLTLFAVWAAINAFNMVDGIDG
                     LLGGLSCVSFAAIGMILWFDGQTSLAIWCFAMIAAILPYIMLNLGILGRRYKVFMGDA
                     GSTLIGFTVIWILLETTQGKTHPISPVTALWIIAIPLMDMVAIMYRRLRKGMSPFSPD
                     RQHIHHLIMRAGFTSRQAFVLITLAAALLASIGVLAEYSHFVPEWVMLVLFLLAFFLY
                     GYCIKRAWKVARFIKRVKRRLRRNRGGSPNLTK"
     gene            complement(16673..17932)
                     /gene="rho"
                     /locus_tag="OO357_001693"
     CDS             complement(16673..17932)
                     /gene="rho"
                     /locus_tag="OO357_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017492510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HCR5886669.1"
                     /translation="MNLTELKNTPVSELITLGENMGLENLARMRKQDIIFAILKQHAK
                     SGEDIFGDGVLEILQDGFGFLRSADSSYLAGPDDIYVSPSQIRRFNLRTGDTISGKIR
                     PPKEGERYFALLKVNEVNFDKPENARNKILFENLTPLHANSRLRMERGNGSTEDLTAR
                     VLDLASPIGRGQRGLIVAPPKAGKTMLLQNIAQSIAYNHPDCVLMVLLIDERPEEVTE
                     MQRLVKGEVVASTFDEPASRHVQVAEMVIEKAKRLVEHKKDVIILLDSITRLARAYNT
                     VVPASGKVLTGGVDANALHRPKRFFGAARNVEEGGSLTIIATALIDTGSKMDEVIYEE
                     FKGTGNMELHLSRKIAEKRVFPAIDYNRSGTRKEELLTTQEELQKMWILRKIIHPMGE
                     IDAMEFLINKLAMTKTNDDFFEMMKRS"
     gene            complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="OO357_001694"
     CDS             complement(18017..18118)
                     /gene="rhoL"
                     /locus_tag="OO357_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rho operon leader peptide RhoL"
                     /protein_id="HCR5886670.1"
                     /translation="MRSEQISGSSLNPSCRFSSAYSPVTRQRKDMSR"
     gene            complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="OO357_001695"
     CDS             complement(18259..18588)
                     /gene="trxA"
                     /locus_tag="OO357_001695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017375425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxA"
                     /protein_id="HCR5886671.1"
                     /translation="MSDKIIHLTDDSFDTDVLKADGAILVDFWAEWCGPCKMIAPILD
                     EIADEYQGKLTVAKLNIDQNPGTAPKYGIRGIPTLLLFKNGEVAATKVGALSKGQLKE
                     FLDANLA"
     gene            18719..19984
                     /gene="rhlB"
                     /locus_tag="OO357_001696"
     CDS             18719..19984
                     /gene="rhlB"
                     /locus_tag="OO357_001696"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlB"
                     /protein_id="HCR5886672.1"
                     /translation="MSKTHLTEQKFSDFALHPKVVEALEKKGFHNCTPIQALALPLTL
                     AGRDVAGQAQTGTGKTMAFLTSTFHYLLSHPAIADRKVNQPRALIMAPTRELAVQIHA
                     DAEPLAEATGLKLGLAYGGDGYDKQLKVLESGVDILIGTTGRLIDYAKQNHINLGAIQ
                     VVVLDEADRMYDLGFIKDIRWLFRRMPPANQRLNMLFSATLSYRVRELAFEQMNNAEY
                     IEVEPEQKTGHRIKEELFYPSNEEKMRLLQTLIEEEWPDRAIIFANTKHRCEEIWGHL
                     AADGHRVGLLTGDVAQKKRLRILDEFTRGDLDILVATDVAARGLHIPAVTHVFNYDLP
                     DDCEDYVHRIGRTGRAGASGHSISLACEEYALNLPAIETYIGHSIPVSKYNPDALMTD
                     LPKPLRLTRPRTGNGPRRTGAPRNRRRSG"
     gene            20118..21602
                     /gene="gppA"
                     /locus_tag="OO357_001697"
     CDS             20118..21602
                     /gene="gppA"
                     /locus_tag="OO357_001697"
                     /EC_number="3.6.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanosine-5'-triphosphate,3'-diphosphate
                     diphosphatase"
                     /protein_id="HCR5886673.1"
                     /translation="MGSTSSLYAAIDLGSNSFHMLVVREVAGSIQTLTRIKRKVRLAA
                     GLNSENDLSNEAMERGWQCLRLFAERLQDIPPSQIRVVATATLRLAVNAGDFIAKAQE
                     ILGCPVQVISGEEEARLIYQGVAHTTGGADQRLVVDIGGASTELVTGTGAQTTSLFSL
                     SMGCVTWLERYFADRNLGQENFDAAEKAAREVLRPVADELRYHGWKVCVGASGTVQAL
                     QEIMMAQGMDERITLEKLQQLKQRAIHCGRLEELEIDGLTLERALVFPSGLAILIAIF
                     TELNIQCMTLAGGALREGLVYGMLHLAVEQDIRSRTLRNIQRRFMIDIDQAQRVAKVA
                     ANFFDQVENEWHLEAISRDLLISACQLHEIGLSVDFKQAPQHAAYLVRNLDLPGFTPA
                     QKKLLATLLLNQTNPVDLSSLHQQNAVPPRVAEQLCRLLRLAIIFASRRRDDLVPEMT
                     LQANHELLTLTLPQGWLTQHPLGKEIIAQENQWQSYVHWPLEVH"
     gene            complement(21649..23670)
                     /gene="rep"
                     /locus_tag="OO357_001698"
     CDS             complement(21649..23670)
                     /gene="rep"
                     /locus_tag="OO357_001698"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase Rep"
                     /protein_id="HCR5886674.1"
                     /translation="MRLNPGQQQAVEFVTGPCLVLAGAGSGKTRVITNKIAHLIRGCG
                     YQARHIAAVTFTNKAAREMKERVGQTLGRKEAHGLMISTFHTLGLDIIKREYAALGMK
                     ANFSLFDDTDQLALLKELTEGLIEDDKVLLQQLISTISNWKNDLKTPSQAAASAIGER
                     DRIFAHCYGLYDAHLKACNVLDFDDLILLPTLLLQRNEEVRERWQNKIRYLLVDEYQD
                     TNTSQYELVKLLVGSRARFTVVGDDDQSIYSWRGARPQNLVLLSQDFPALKVIKLEQN
                     YRSSGRILKAANILIANNPHVFEKRLFSELGYGTELKVLSANNEEHEAERVTGELIAH
                     HFVNKTQYKDYAILYRGNYQSRVFEKFLMQNRIPYKISGGTSFFSRPEIKDLLAYLRV
                     LTNPDDDSAFLRIVNTPKREIGPATLKKLGEWAMTRNKSMFTASFDMGLSQTLSGRGY
                     EALTRFTHWLAEIQRLAEREPIAAVRDLIHGMDYESWLYETSPSTKAAEMRMKNVNQL
                     FSWMTEMLEGSELDEPMTLTQVVTRFTLRDMMERGESEEELDQVQLMTLHASKGLEFP
                     YVYMVGMEEGFLPHQSSIDEDNIDEERRLAYVGITRAQKELTFTLCKERRQYGELVRP
                     EPSRFLLELPQDDLIWEQERKVVSAEERMQKGQSHLANLKAMMAAKRGK"
     gene            23757..24038
                     /gene="ppiC"
                     /locus_tag="OO357_001699"
     CDS             23757..24038
                     /gene="ppiC"
                     /locus_tag="OO357_001699"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004097481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase PpiC"
                     /protein_id="HCR5886675.1"
                     /translation="MAKTAAALHILVKEEKLALDLLVQIKNGADFGKLAKKHSICPSG
                     KRGGDLGEFRQGQMVPAFDKVVFSCPVLEPTGPLHTQFGYHIIKVLYRN"
     gene            complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="OO357_001700"
     CDS             complement(24084..25559)
                     /gene="ilvC"
                     /locus_tag="OO357_001700"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HCR5886676.1"
                     /translation="MANYFNTLNLRQQLAQLGKCRFMGRDEFADGASYLQGKKVVIVG
                     CGAQGLNQGLNMRDSGLDISYALRKEAIAEKRASWRKATENGFKVGTYEELIPQADLV
                     VNLTPDKQHSDVVRTVQPLMKDGAALGYSHGFNIVEVGEQIRKDITVVMVAPKCPGTE
                     VREEYKRGFGVPTLIAVHPENDPKGEGMAIAKAWAAATGGHRAGVLESSFVAEVKSDL
                     MGEQTILCGMLQAGSLLCFDKLVEEGTDPAYAEKLIQFGWETITEALKQGGITLMMDR
                     LSNPAKLRAYALSEQLKEIMAPLFQKHMDDIISGEFSSGMMADWANDDKKLLTWREET
                     GKTAFETAPQYEGKIGEQEYFDKGVLMIAMVKAGVELAFETMVDSGIIEESAYYESLH
                     ELPLIANTIVRKRLYEMNVVISDTAEYGNYLFSYACVPLLKPFMAELQPGDLGKAIPE
                     GAVDNAQLRDVNEAIRSHAIEQVGKKLRGYMTDMKRIAVAG"
     gene            25709..26599
                     /gene="ilvY"
                     /locus_tag="OO357_001701"
     CDS             25709..26599
                     /gene="ilvY"
                     /locus_tag="OO357_001701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709578.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator IlvY"
                     /protein_id="HCR5886677.1"
                     /translation="MDLRDLKTFLHLAESRHFGRSARAMHVSPSTLSRQIQRLEEDLG
                     QPLFVRDNRTVTLTEAGEELRVFAQQTLLQYQQLRHTIDQQGPLLYGELHIFCSVTAA
                     YSHLPPILDRFRAEHPSVEIKLTTGDAADAMEKVVTGEADLAIAGKPETLPGAVAFSM
                     LENLAVVLIAPALPCPVRNQVSVEKPDWSTVPFIMANQGPVRRRIELWFRRNKISNPM
                     IYATVGGHEAMVSMVALGCGVALLPEVVLENSPEPVRNRVMILERSDEKTPFELGVCA
                     QKKRLHEPLIEAFWKILPNH"
     gene            complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="OO357_001702"
     CDS             complement(26596..28140)
                     /gene="ilvA"
                     /locus_tag="OO357_001702"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase, biosynthetic"
                     /protein_id="HCR5886678.1"
                     /translation="MADSQPLSGAPEGAEYLRAVLRAPVYEAAQVTPLQKMEKLSSRL
                     DNVILVKREDRQPVHSFKLRGAYAMMAGLTEEQKAHGVITASAGNHAQGVAFSSARLG
                     VKALIVMPTATADIKVDAVRGFGGEVLLHGANFDEAKAKAIELSQQQGFTWVPPFDHP
                     MVIAGQGTLALELLQQDAHLDRVFVPVGGGGLAAGVAVLIKQLMPQIKVIAVEAEDSA
                     CLKAALDAGHPVDLPRVGLFAEGVAVKRIGDETFRLCQEYLDDIITVDSDAICAAMKD
                     LFEDVRAVAEPSGALALAGMKKYIAQHNIRGERLAHILSGANVNFHGLRYVSERCELG
                     EQREALLAVTIPEEKGSFLKFCQLLGGRSVTEFNYRFADAKNACIFVGVRLSRGLEER
                     KEILQMLNDGGYSVVDLSDDEMAKLHVRYMVGGRPSHPLQERLYSFEFPESPGALLRF
                     LNTLGTHWNISLFHYRSHGTDYGRVLAAFELGDHEPDFETRLNELGYDCHDETNNPAF
                     RFFLAG"
     gene            complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="OO357_001703"
     CDS             complement(28143..29993)
                     /gene="ilvD"
                     /locus_tag="OO357_001703"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HCR5886679.1"
                     /translation="MPKYRSATTTHGRNMAGARALWRATGMTDADFGKPIIAVVNSFT
                     QFVPGHVHLRDLGKLVAEQIEAAGGVAKEFNTIAVDDGIAMGHGGMLYSLPSRELIAD
                     SVEYMVNAHCADAMVCISNCDKITPGMLMASLRLNIPVIFVSGGPMEAGKTKLSDQII
                     KLDLVDAMIQGADPKVSDSQSDQVERSACPTCGSCSGMFTANSMNCLTEALGLSQPGN
                     GSLLATHADRKQLFLNAGKRIVELTKRYYEQNDESALPRNIASKAAFENAMTLDIAMG
                     GSTNTVLHLLAAAQEAEIDFTMSDIDKLSRKVPQLCKVAPSTQKYHMEDVHRAGGVIG
                     ILGELDRAGLLNRDVKNVLGLTLPQTLEQYDVMLTQDDAVKNMFRAGPAGIRTTQAFS
                     QDCRWDSLDDDRANGCIRSLEHAYSKDGGLAVLYGNFAENGCIVKTAGVDDSILKFTG
                     PAKVYESQDDAVEAILGGKVVAGDVVVIRYEGPKGGPGMQEMLYPTSFLKSMGLGKAC
                     ALITDGRFSGGTSGLSIGHVSPEAASGGSIGLIEDGDLIAIDIPNRGIQLQVSDAELA
                     ARREAQEARGDKAWTPKNRERQVSFALCAYASLATSADKGAVRDKSKLGG"
     gene            complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="OO357_001704"
     CDS             complement(30058..30987)
                     /gene="ilvE"
                     /locus_tag="OO357_001704"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain-amino-acid transaminase"
                     /protein_id="HCR5886680.1"
                     /translation="MTTKKADYIWFNGEMVRWEDAKVHVMSHALHYGTSVFEGIRCYD
                     SHKGPVVFRHREHMQRLHDSAKIYRFPVSQSIDELMEACRDVIRKNNLTSAYIRPLIF
                     VGDVGMGVNPPAGYSTDVIIAAFPWGAYLGAEALEQGIDAMVSSWNRAAPNTIPTAAK
                     AGGNYLSSLLVGSEARRHGYQEGIALDVNGYISEGAGENLFEVKDGVLFTPPFTSSAL
                     PGITRDAIIKLAKELGIEVREQVLSRESLYLADEVFMSGTAAEITPVRSVDGIQIGEG
                     RCGPVTKRIQQAFFGLFTGETEDKWGWLDQVNQ"
     gene            complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="OO357_001705"
     CDS             complement(31007..31270)
                     /gene="ilvM"
                     /locus_tag="OO357_001705"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 small subunit"
                     /protein_id="HCR5886681.1"
                     /translation="MMQHQVNVSARFNPETLERVLRVVRHRGFHVCSMNMAAASDAQN
                     INIELTVASPRSVDLLFSQLNKLVDVAHVAICQSTTTSQQIRA"
     gene            complement(31267..32913)
                     /locus_tag="OO357_001706"
     CDS             complement(31267..32913)
                     /locus_tag="OO357_001706"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001012636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase 2 catalytic subunit"
                     /protein_id="HCR5886682.1"
                     /translation="MNGAQWVVHALRAQGVNTVFGYPGGAIMPVYDALYDGGVEHLLC
                     RHEQGAAMAAIGYARATGKTGVCIATSGPGATNLITGLADALLDSIPVVAITGQVSAP
                     FIGTDAFQEVDVLGLSLACTKHSFLVQSLEELPRIMAEAFDVASSGRPGPVLVDIPKD
                     IQLASGDLEPWFTTVENEVTFPHAEVEQARQMLAKAQKPMLYVGGGVGMAQAVPALRE
                     FLAATKMPATCTLKGLGAVEADYPYYLGMLGMHGTKAANFAVQECDLLIAVGARFDDR
                     VTGKLNTFAPHASVIHMDIDPAEMNKLRQAHVALQGDLNALLPALQQPLNINDWQQHC
                     AQLRDEHSWRYDHPGDAIYAPLLLKQLSDRKPADCVVTTDVGQHQMWAAQHIAHTRPE
                     NFITSSGLGTMGFGLPAAVGAQVARPNDTVVCISGDGSFMMNVQELGTVKRKQLPLKI
                     VLLDNQRLGMVRQWQQLFFQERYSETTLTDNPDFLMLASAFGIPGQHITRKDQVEAAL
                     DTMLNSDGPYLLHVSIDELENVWPLVPPGASNSEMLEKLS"
     gene            complement(32916..32966)
                     /locus_tag="OO357_001707"
     CDS             complement(32916..32966)
                     /locus_tag="OO357_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886683.1"
                     /translation="MNNSTKFCFSRFRTGN"
     gene            complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="OO357_001708"
     CDS             complement(33052..33150)
                     /gene="ilvL"
                     /locus_tag="OO357_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ilv operon leader peptide"
                     /protein_id="HCR5886684.1"
                     /translation="MTALLRVISLVVISVVVIIIPPCGAALGRGKA"
     gene            33502..34981
                     /locus_tag="OO357_001709"
                     /pseudo
     CDS             33502..34981
                     /locus_tag="OO357_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418214.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
     gene            complement(35005..35343)
                     /gene="maoP"
                     /locus_tag="OO357_001710"
     CDS             complement(35005..35343)
                     /gene="maoP"
                     /locus_tag="OO357_001710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ori organization protein MaoP"
                     /protein_id="HCR5886685.1"
                     /translation="MAESFTTTNRYFDNKHYPRGFSRHGDFTIKEAQLLERHGYAFNE
                     LDLGKREPVTEEEKLFVAVCRGEREPVTEAERVWSKYMTRIKRPKRFHTLSGGKPQVE
                     GAEDYTDSDD"
     gene            35462..36301
                     /gene="hdfR"
                     /locus_tag="OO357_001711"
     CDS             35462..36301
                     /gene="hdfR"
                     /locus_tag="OO357_001711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator HdfR"
                     /protein_id="HCR5886686.1"
                     /translation="MDTELLKTFLEVSRTRHFGRAAESLYLTQSAVSFRIRQLENQLG
                     VNLFTRHRNNIRLTAAGEKLLPYAETLMSTWQAARKEVAHTSRHNEFSIGASASLWEC
                     MLNQWLGRLYQNQDAHTGLQFEARIAQRQSLVKQLHERQLDLLITTEAPKMDEFSSQL
                     LGYFTLALYTSAPSKLKGDLNYLRLEWGPDFQQHEAGLIGADEVPILTTSSAELAQQQ
                     IAMLNGCTWLPVSWARKKGGLHTVVDSTTLSRPLYAIWLQNSDKNALIRDLLKINVLD
                     EVY"
     gene            complement(36397..36472)
                     /locus_tag="OO357_001712"
     tRNA            complement(36397..36472)
                     /locus_tag="OO357_001712"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36437..36439),aa:Trp,seq:cca)
     gene            complement(36481..36557)
                     /locus_tag="OO357_001713"
     tRNA            complement(36481..36557)
                     /locus_tag="OO357_001713"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(36521..36523),aa:Asp,seq:gtc)
BASE COUNT         8940 a        10016 c         9220 g         8420 t
ORIGIN      
        1 gccaccgcac gtaaaggctt tgccggatac gatcacccaa accagtatcc acagaacgct
       61 gcaatttatt gaatttgcac gatcatgtag gccggataag gcgtttacgc cgcatccggc
      121 aatcaaccgc aggcggcttc caaatggccg cttttttact tattcaccac catcaaatgt
      181 gcctgcataa tgtcactggc cgggcgaccg tgcgccagca aatcagccat tgctttaaga
      241 tatggcggta gatggcggaa ataacgctga tacccggcac acaaataatt cagccccggt
      301 ttgccgctgg catcgagcat gaagcggtgt ttcgggcagc ctccccaaca tgcttttaac
      361 acgttacaac tgcgacactg cgccggtaat tgcttaaatt tatcttcacc aaacacctgc
      421 tgttgcgggg aatcgatcat ttctgcaatt gtttgctgat gcatattccc cagccgatat
      481 tgcggataaa catagtgatc gcaggcgtaa acgtcgccgt tgtgctcaac aatcaccgag
      541 cgcccacagg ttggctgatg atggcaaacc gcacccggcg caccgacaaa attggcaaac
      601 gcccattcga tattcatcac gaaaatcttg ccgacgtcgc gtttgatcca gtggtcaaaa
      661 atcgccacca gaaattcacc aaattcatcg gggcgcacag accattccgt cagttccccc
      721 tgaatatcac caggcgcatg tagtttcagt ccatcgcgga cagctgtttc atcagccagg
      781 cgctcgacca ccggaataaa ctggatgaat tcgactcccg catcgcacaa aaaatcataa
      841 acctgtaacg gttgctgcgc gctggtgcgg ttgacgcaga ccagcacgtt atagtcgaca
      901 tgatgttttt gcaggagcgt cagggcacgc atcaccagtt tatgcgtggg tctgccgcct
      961 ttggtcacgc gatattgatt gtggatctca gccggaccat ccagcgataa cccaacaaga
     1021 aaattatttt ctgccagaaa tgcacaccat tcgtcatcga gcagcacgcc gttagtctgg
     1081 aaactgttac ttatcttcct gccagcacca tatttcgcct gtagcgccac ggcacggcgg
     1141 taaaactcca gcccgagtag cgttggttcg ccgccctgcc aggtaaaagc cacttcgttt
     1201 tgcagttcgc tggcggcgat atagtgacgg atatacgctt ccagcgtgtc atcgtccata
     1261 tgcgtgactg gcttttcgcg gtaaagggat tgtttttcga gataaaaaca gtagtcacag
     1321 ttcagattac aatcggaacc gctcggtttc gctatcacat gaaaagcacg cgttggaacc
     1381 tgttgcagca tggtcgctcc ttgcctgaag tcatccttca atacgttaag ggcgaaatca
     1441 taccgtgggg caatgagtta ttacgggcgt ttattgcgaa gggagggtga cgaaatgcac
     1501 agaaaacaaa aaaccccgcc gaagcggggt tcaaaattgg tcggcgagag aggattcgaa
     1561 cctccgaccc actggtccca aaccagttgc gctaccaagc tgcgctactc gccgaaatac
     1621 tgctttttga atttttagtt caattcttta aagtcgtggt gcgagggggg ggacttgaac
     1681 ccccacgtcc gtaaggacac taacacctga agctagcgcg tctaccaatt ccgccacctt
     1741 cgcattgtca caacttctaa taatggggtg gctaatggga ttcgaaccca cgacaactgg
     1801 aatcacaatc cagggctcta ccaactgagc tatagccacc actacaaatc ttgttacgcg
     1861 gtattactac caccgcagct caagcgctgg gactaaatgg cgcgcccgac aggattcgaa
     1921 cctgagacct ctgcctccgg agggcagcgc tctatccagc tgagctacgg gcgcttagcg
     1981 ccgttgcggg ggtggataat acggacttcc cacccctctg tctagtccct ttttaaataa
     2041 attgctcgtt tggttacggt ttgtgcgttt tgctgcttat tcctccagtt tatgcgcttt
     2101 accgtggcga ttaagcccaa aaactttata aatcgccgtc accgccagca taaagatgat
     2161 gccgacaaac agcgacatac gcgtatcttc attaaagtac atgccgatca aaacgcaaat
     2221 caggaatgcc attgttacgt agttggccca cgggaacagg attgagcgga acggatggct
     2281 ggcaatcgcc gctttatgtg cacgccgaaa acgcagctgg cttatcaata tcacaaacca
     2341 tggcaccatc cccggaagca cgctggcact gtagacgtag acaaacacac gctgcggatt
     2401 gggaatgatg tagttcaggc atgagccaat tagcagaata gcaatagata ctgccacacc
     2461 cgcaaccggt acgccgtgac gggaaacttt tgccattgcc gccggtaact gacggttttt
     2521 cgccagtgcg tagagcatac gtccgcaact gtacatgccg ctgttacagc cagagagcgc
     2581 agccgtcagc accacaaagt tgataatgcc cgccgctgcg gtaataccga ttttggcaaa
     2641 agtcagtacg aacgggctgc cgttgctgcc aatttcattc cacgggaaga tggtgacgat
     2701 aacgaaaatc gcgcctacgt agaaaatcag gatccgccac agcactttgc ctacggcact
     2761 gcgcagcgtc acctgcggat tcttcgcttc accggcagta atgccaatca gctccacgcc
     2821 ctggtaggac gccaccacaa tacacagagc ggtcaggaac cctttccagc cacccgcaaa
     2881 gaaaccgcca tgctctgtga gattgctaaa accaatcgac tgcccgccat tgccaaagcc
     2941 aaagaaaatc acgcccaggc caatgacaat catcacgata atcgtggtga ctttgatcat
     3001 cgcgaaccag aactcgattt cgccgtacaa ccgtaccgcc gccagattcg ccaacgccac
     3061 cagcgccacc gcgatcaatg cgggtatcca ctgcgccatc tccggggacc aaaactggac
     3121 ataaacgcca atggcggtga tttctgagat ccccaccgcc atccacataa accagtaaga
     3181 ccaggcggtg agatagccaa aaaacgggct catataacga tgcgcataaa cggcgaacga
     3241 accggtaacc ggttcgagga acaacatttc gcccattgaa cgcatgatga aaaaaacgaa
     3301 cagcccggcg atgatatagg ccaacaatac ggatggcccg gcccatttca gggtactggc
     3361 ggcccccata aacaggccga cgccaatggt gccccccagg gcgatgagtt cgatatgtcg
     3421 agcttccagc ccacgctgta gctctggttt gttatctgcc ataaatcctc gtgttgtgtt
     3481 tgcatgcttt ccggtgttac cggttatcgt tatgggtaca tcgagtgttg cgaatgtttt
     3541 cgtaattcag gagaaatggc aaataaagca ttaaaaattt gaatgctttg tgtaataaaa
     3601 aagcagacag gcgacgaagt gaccactccg tcgctttaca aagagaggaa aatcagaggt
     3661 tgccggtgta gtgccagcgt aaataacgca gcaaacgaag ctgacgctta atgcggctcg
     3721 gctgcgaaag caggcggtag agccactcca gtcccagcgt ttgccagatt ttcggtgcgc
     3781 gttttacgtg accggtgaaa acatcgtaag tcccgccaac gcccatatac agcgcatctg
     3841 gatgcaccag acggcagtcg cgcatgaaga tctcctgctt tggcgatccc atcgcaacgg
     3901 tgacgatttg cgctccgctg gcatgaatgc gttcaaacag cgcctgacgc tgctcgggtt
     3961 taaaataacc atcctgactg ccaacgatat tcacattcca ttggttgcgc agtttagctt
     4021 cagtttgcgc cagcacttca ggtttaccgc ccacaagaaa taccggcgtc ccttctttgc
     4081 ctgcgcgcgc catcagctct tcccagagat cggcaccggc aacgcgggaa acctgcgcct
     4141 gcgggtactt tttacgtact gaacgtacaa tgctgatgcc atccgcatat ttaaattcgg
     4201 cagcgttaat taactccctg acctcggcgt tatcttcaat ggtcagcatt ttttcggcat
     4261 taatggcaac cagcgttccc tgcttaagct gcccgtcagc aaacagataa tcgagggcat
     4321 gctgcatatc acgccaacca atcaactgta agccacgcag cgtataggtc ggtgccgtgg
     4381 tgttgttatt cattgttatc cttcaacctg cgtccggagc gatgattttg tacgtttatg
     4441 aatgagtccg gcgctttcaa aaagccagta caacagtttt gcgatcatca gacatgcgcc
     4501 gaagaccacg ataaaaaaga ctacgcgtga gacaaacgaa tccagccctt cacgtgccag
     4561 cacgatcata ttgaagatcg ccccaaagca gaaactgtgc aatatcgcag ccttatagcg
     4621 attagtctcg cggttgccca gctcatacag ccagtcgaac catttgatga tcagtccaac
     4681 cacaatcgcc ccgagtggga tgaacaacgc gccgcccatc accaccagtg agcctataag
     4741 cgtaggcgag atcgccagtc cggagtggtt attcagtact tcccaggtaa agtaattggc
     4801 tgagttcagc accatactcg ggcgccccgg ccacagccag gaagggataa agacatagaa
     4861 atcgcggaca attggagcca ggccctggaa gtcgatgttg tcgtagttct gcaacagcaa
     4921 cgccagattc tcccacggtg aaaaggtgtc gcgagtgaga tagagaaacg tatagaacgc
     4981 ttcatcgccg ctcacattca ttccatagcg ttttaacgcc agccagaaca tgccaacaat
     5041 ccccagcacg cccgccgccg ccagcatcca caacgaaatc cagccgcgaa taatgccaat
     5101 aaacaggaag atagcgaatg cgatgatgat attggcgcga gtgccgccga caatcatata
     5161 agtcagcaag ccaaaggcga ccgtgctgac gaggaaaaac agccacgctt tgctgtcctg
     5221 gcgcagaaag tagaccacca gcatcgccgg gatgaaaaag taaaagaagc gttttaacgc
     5281 cacgccggag acttcactgg aaaagatctg actgtaggag ttaagccgga acagcaaaaa
     5341 gccgttgtgc atgaagaaga tgccgacgct taccagcgcg atacccatca ggatcaccca
     5401 cgtaagattg gtctccacgc ggttcatggt aaacagcgga cggcgcggta catcagcaac
     5461 gcgtttgcgt aggcgggttt tgtaggtgac atagtaaacc gcgtagaagc agcccgcaga
     5521 aagcaacacc tgcaacaaga tttctggcgg cgcgacacca acatcaaagc gaaataccag
     5581 cacgctggtc agcgggaagc cgaagaaaaa ggtgagcaaa aacagcaatg aaaagaagac
     5641 attgaagtta aagcgcacac ggcgaaactc aaaccaggtc agcgtggcaa taaacagcgt
     5701 gcagagcagc cagaccacaa acaggccact gaattgcagc agactcatgc gacctccccg
     5761 gcggcaatcg ccaacgcccg ctgccagcct tgtagatagt tagggctaaa gaaggcaatg
     5821 gtgtttttat ccgccgacgc caactggcgc tgcgcttcac gcacaatatc ctcgttgaga
     5881 tcgtcggtag taaacagcac tggcaaatgc tgttccgtca tatcctgcca gaacggattt
     5941 tccctgttaa gcacacaagg aatgcccgcc tgaatcagta agcaaagcgt accaatgccc
     6001 tgctggcggg caaaaataaa gtaaccaaga tcgcactgac gaagtagcgc cagataggcg
     6061 tcaaattcca gtttttcgct caaaatttgc agattttctt cgctgaataa ctccagcccc
     6121 gcctgacgaa cttcctcaat gtacgcttcg ttattaggcg gatatcccat cggcaccacc
     6181 acttttaccg tatcgccaaa ttgctgatga acggcacgta aagcggcgac atgctcattg
     6241 ctgcggtcgc cggagttccc caccagaatg gtcatttttc cttcacgttg ccgatcgttc
     6301 gccatcgtat tgagagaagg gtccatccgc gtcgggaagt acagcagttc tccccgcacc
     6361 tttgggtgtg ttttggcaaa aaagctcaaa tcgccgcggg tggcaaatac acagccgact
     6421 cgcttttgcg ccaggcgacg tagtgggtaa aaaagcttat atctcaagcc actggaaagc
     6481 tcatacaggt ctgcccccca gatatgccag aaaaactggc tgggcttaat gccaccactc
     6541 agcagagcca gccacagtgt gggattgaac tgaccgtgga aaaaaaatcg ctgctgacgg
     6601 ttagcttttg ctttcgcgat gaccgcttcc gccagcgatt ttttcccagg gaaaaattgc
     6661 acagaaagcg ccggacagct atcacttaag ccgtcgtcct tgccaacaac cataaactcg
     6721 cgcgcgtgct cgctcgtcgc ggccagcgcg tcattgaaaa accgcaaaac ggttcggtta
     6781 tggtgaggga tatccgatcc cagtacgtga atcagtacag tcatgcccgc ctacgccaga
     6841 gtaaaaacac gccacaacaa agagaaaaat agacgatata agttgccata tatgcctgcg
     6901 ccgcgcccag tgcaccatgc gcagggatta gccagtgggc aaataccatc aataaagtga
     6961 actggctgac ttccgccaga atataaaacc gcagtgacgc tttggcgatc accagataac
     7021 caaagacata agcgcccact tttaacacat cacccactaa ctgccaggca aagagatcgc
     7081 gcatagcggt aaatttattc gacaacagca accagatagc aaaatcacgc agcaaccaga
     7141 cggtaaaact cgctgccgcc actgccggta agacgaattt cagcgattta accacttccc
     7201 gggtgatatc gcgcttttcc gttagccgcg acaacgtggg cagcaaatat acgctgaacg
     7261 atgccgtaat aaattgcagg taggcatcgg aaatactgct caccccttgc cagatcccca
     7321 cttcatccca gctatactgc gccgccagca gtttacgcat catgatgtaa gcaacaggca
     7381 aggtcaccga cgtaatcaac gccatgagcg taaatttgct caactgccct gccagaccgt
     7441 tatcccagct gggtttcaga tagcttaacg ggatgacacc acgtttaatc aacatgatgg
     7501 cggcaggaat taccaccagc gcgggaatca gcgccagacc cagcaacgcc ccttcataac
     7561 cgcccaaacg gtaactgacg tagtacgcga gaacgccaat caagctgccg acaatcagcg
     7621 ataacgcatt acctgcggca tcgcgaaagc ctttcatcag cgccagtaac aggttgcccc
     7681 aggcgatccc catttgcacc agcgccacta aacgcaccag cccctgatag tcggtattac
     7741 caaacaatcc ctggctgatt ggcgcagctg ccagcacaaa aaccagcgcc atcagcgtag
     7801 agaaaccaag taccatcgct gatgaagtgc cgaccacgcg gcgcagctgt tgcggattat
     7861 catggtactg ggcaacgtat ttggttacac cgttaaagat gccagccccg gcaagcacgc
     7921 cgagcacggt aatcaactgg cggaaatttg ccgccagccc aagccccgcc ggaccaaatg
     7981 acaccgccag caacttaccg accagtaacc cggcaccaat cttgaccagt gtactggccg
     8041 ccgtccacaa ggacgctttt gccaacgaca tatcaggaga agtagttcaa caaagtcgca
     8101 attaccgtac gctgattgac gggcgacagg ttgtagaaca gcggcaggcg cagcaggcgc
     8161 tcgctctctt tggtggtgta gcgatcttca ccgtggaact caccaaagtg ttcccccgca
     8221 gggcaaccgt gcagcggaat gtaatgaaac accgccatga tttccgcttc tttcagaaag
     8281 ttaatcaacg cgctccggtc atcaatatcc cgcagtttaa tgtagaacat atgcgcgttc
     8341 tgcacgcagc catcgggaat cgacggcagc tcgatacgcc cggctttcgc cagaggcgct
     8401 aacgtatcgt agtagttttg ccacagcgcc agacgttgct ggttgatacg atccgctgct
     8461 tccagttgcg cccacaggta cgcagcttgc agatcggaca tcaaatagct ggagccaata
     8521 tcgcgccagg tatatttatc gacctgacca cggaagaact ggctgcggtt agtgcccttt
     8581 tcacggatga tctcggctcg ttcgattaac gctttatcgt taatcagcgt cgcgccgcct
     8641 tcaccacccg ccgtgtagtt tttggtttca tggaagctaa agcagccaat atgaccaatg
     8701 gttcccagtg cacgcccttt gtaagtggac atcacgccct gagcggcatc ttccaccaca
     8761 aacaaattat gctttttcgc caacgccata atggtgtcca tttcgcaggc cacacccgcg
     8821 taatggaccg gcacgataac gcgcgttttg tcggtgatcg ctgcttcaat caacgtttcg
     8881 tcgatgttca tggtgtccgg gcgaacatcc acaaagacga ttttcgcgcc acgcagcaca
     8941 aaggcattgg cggtggagac aaaggtgtag ctcggcatga tcacttcatc gccaggctgg
     9001 atatcgagca gcagcgccgc catctccagc gaagcggtgc aggacggcgt cagtaacact
     9061 ttggcgctgc caaaacgttg ctccagccac tgctggcagc gacgggtaaa accgccatcg
     9121 ccacacagtt tgccgctacc cattgccgac tgcatatagt cgagttcggt tcccaccacc
     9181 ggcggtgcgt taaatggaat catgtgatca cctgtataac cagtacgcgg tgctttctac
     9241 attcgcacca ctttgtatgt atcgtttaag cgcggcggtg ttgcccattt gggtcgccac
     9301 ccgcaaagtt gttttaccgc gagcatacgc ccagtttagc gccgtttgca tcagctcagc
     9361 acctgcaccg cgtccagcca gcaggccaat tcgcgcatct gtcgcattga gttcccgtaa
     9421 agagacatag ccgcgaatat cgccggacgc cgcacgtaga atcagacatt gatgatcaaa
     9481 ggtgccgcgc acggcatttt caatccactg tgcataaaag cgaccgctgg cgtcaggcgc
     9541 ataccacggc gcacgaaaac ggctttgcgc aaatgcggcg ctagctaact gacgtaatgc
     9601 gggaatatcg gtctcttgtg ccacgacagc accgctatca ctgacattgt tcacgggtag
     9661 cgccaaatca acttcacctt ctaccaggga gaatcccagc tgttgcaggg catccagttc
     9721 ccccgtattt gatgccgcaa ttttggcctg cacccgtgac catggcgcta aggcgtctga
     9781 cgtcaggagc ggtgcttcag acgtaatgcg cacgatggcg ctgttaacac caaagaaggc
     9841 gttttcccag gttagtggct caatactggc gcggacgggc acgaagtaac tccagcagat
     9901 attggccgta gccagttttc gctaatgaac tggcagcacg cttcacaccc tcgtcatcga
     9961 gccagccgtt acgccaggcg atctcttcca ggcaggcaat cttaaagccc tggcgttttt
    10021 ctactgtctg cacaaaagtg ctggcctcaa tcaggctgtc gtgagtgccg gtatccagcc
    10081 aggcaaatcc gcgcccgagc agttcaacgg tcaggttgcc cgcctcgagg tacatctggt
    10141 tgatggaggt aatctccagt tcaccgcgct ccgacggctt cacctgcttt gcgtactcca
    10201 cgactttact gtcgtagaaa taaagcccgg tcaccgccca gtttgacttc ggctgttttg
    10261 gcttttcttc aagcgagata gcgcggaaat tgtcgtcaaa ttccaccacg ccaaagcgtt
    10321 ccgggtccat cacctgatag ccaaaaaccg tcgccccttc cgtgcgcgcc gcaacatgac
    10381 gcagcttcgg actgaagccc tgaccgaaga agatgttatc gcccagcacc agacaagaag
    10441 gttcaccatt aaggaaggtt tcaccgatga taaaggcctg cgccagaccg tccgggctgg
    10501 gctgttcggc atattccagc tggataccga actcactacc atcgcccagc aggcgctgga
    10561 aataaccttt atcttccggc gtagtgatga tgagaatttc gcggataccg gccagcatca
    10621 gcaccgacag cggatagtaa atcattggct tatcgtaaat cggcaacagt tgcttcgata
    10681 cgccgcgcgt aatcggatgc aatcgggtac cggaaccgcc cgccaggata atacccttca
    10741 tgtttgcctc cgctgaaaat tagcctttca gacctaaacg ctcaccctga tagctgccgt
    10801 cctgcacctg cttccaccag ctttcattag ccagatacca ctgaaccgtt ttacgcattc
    10861 cactttcaaa ggtttcctgc ggcagccagc caagttcacg ggcaattttc gaagcatcaa
    10921 tggcataacg cagatcatgc cccggacggt cagcgacaaa ggtgatcagg tcacgatagt
    10981 acgccacgcc gtgcggcttg ttcgaagcca gttcttccag cagctcgcaa atggtttcca
    11041 caacatcgag attcttacgc tcgttgtgac caccaatatt ataggtttca ccgactttcc
    11101 cggtggtcgc cacgcaatac agcgcgcggg cgtgatcttc cacatacagc cagtcacgga
    11161 tttgctgccc gttgccatat accggcagcg gtttacccgc cagcgcgttg aggatcatca
    11221 gcgggatcag tttttccgga aagtggtaag gaccgtagtt attcgagcag ttggtgatca
    11281 gcgtcggcag accgtaggtc cgcagccagg cgcgcaccag atggtcgctg ctggcttttg
    11341 acgcggaata agggctgctc ggcgcatacg gcgtggtttc ggtgaagaaa tcatccgtcg
    11401 agtgcaggtc accatatact tcgtcggtgg agatatgatg aaaacggaac gctgattttt
    11461 tatcttccgt cagcgtattc cagtaagccc gcgccgcttc aagcaatgta taagtcccga
    11521 caatgttggt ttcaataaac gctgccgggc cgtcaataga acggtcaaca tggctttcgg
    11581 ctgccagatg catgacacag tctggctgat gctcagtgaa tacgcgtgcc agttctgccc
    11641 gatcgcagat atcaactttc tcaaaggcaa agcgctcgct ttgcgcgacc ggtgccagcg
    11701 acatcaggtt tccggcgtag gtcagcttat cgaccactac caccgcatcg ctcgtttcgt
    11761 tgatgatata acgcaccagc gccgagccaa taaacccggc accacctgtt atcagaattt
    11821 ttctcatcag cgccagactc ctttggcatc gacgacatac tgctgatgga cattgtcgcc
    11881 attgataact ttgaactgac tatgatcgac cagcatcacc agcacgtctg ccgttgccag
    11941 cgcctcgtca agctgcgcca gagtacaaag cccggtcagt tttttcggca actggtggat
    12001 gttaggctca acaaccagcg tttcgccgct gtgccactgg gcgatcagtt cagcgatttc
    12061 catcgccggg ctttcgcgca ggtcatcaat attcggttta aacgccagac caaagcaggc
    12121 gattttcagt tcactggctc gtttatcggt agcagcaagg cagtcggcaa ccgccgcttt
    12181 cacctgatcg ataacccaga acggtttgtg atcgttaact tcgcgcgcgg tacggataag
    12241 ccgcgcctgc tgggggttct gtgccacgat aaaccacgga tcaacagcaa tgcagtgacc
    12301 gcccacgcca gggccaggct gaagaatatt aacgcgaggg tgacgattcg ccaggcgaat
    12361 cagttcccag acgttaatcc cctgatcggc acaaatcagc gacaattcat tagcaaaagc
    12421 gatattcaca tcgcggaagc tgttttcggt gagcttacac atttccgccg tccgcgagtt
    12481 agtgacgaca cactcacctt cgaggaaaat tttgtacaat tcgctggcgc gggccgaaca
    12541 aaccggcgtc ataccaccaa tcacgcgatc gtttttaatc agctcgacca taacctggcc
    12601 cggtaataca cgttccgggc agtaagcaat gttgacgtcc gcctgctcgc ccacctgctg
    12661 tgggaaagtc agatccgggc gcatctctgc taaccattct gccatcttct cggttgaccc
    12721 caccggcgag gtggattcaa ggatcaccag cgcgcctttt ttcagcactg gcgcaatgga
    12781 gcgagcagcc gattcaacgt aggtcatatc tggctcatga tcgcctttaa acggcgtagg
    12841 tacagcaatc agccaggcat ccgcttcaac tggcgtcgtg ctcgctcgta aaaaaccgcc
    12901 ttctacggca gtttttacta cgctcgccaa atcaggctcg acgatatgga tttcgccacg
    12961 attgatggta tcaaccgcgt gttggttgat atcgacacca atcacctgtt tttgccgtga
    13021 ggcaaacgct gctgctgtag gcagcccgat gtaacccagt ccgataacag aaatggtcgc
    13081 aaaactcata gtgatatccg attatttttt aacgcttcca gaatgcgaga gcatgcctga
    13141 ccatcaccat acgggttatg ggcgcggctc atagcttgat attcgttttc gtcttttaaa
    13201 agacgcgtca cttcctcgac aattcgctgc ttatccgtgc ctaccagacg caccgtaccc
    13261 gccgtcaccg cttccggacg ctcagtggta tcgcgcatca ccagcaccgg tttccccagc
    13321 gaaggcgctt cttcctgaat gccgcctgag tcagtcaaaa tcagccaggc gtggttcatc
    13381 agccagacaa acggtaaata ctcctgggga tcgatcagaa tgacattttt cacatgcccc
    13441 agaatgcgat tgaccggctc tctgacgttc gggttgagat gcaccggata gacaatctgg
    13501 atgtcctggt gcgtggtggc gatgtctgcc agcgcgtggc agatttcttc aaagccacga
    13561 ccgaaactct cacgcctatg accggtcacc agaatcatct ttttatcggg ggcgataaac
    13621 gggtaatttg ccgccagttc tgaacgcagc gtgtcgctgc tcatcacctg gtcacgcacc
    13681 cataacagtg catcaatgac tgtattaccg gtaatgaaga ttcggctatc cgcaacgttt
    13741 tcacgcagca agttttgccg ggaagtttcg gttggagaga agtgatacat cgccagatgc
    13801 ccggtcaatg tacggttagc ctcttccggc cacggcgaat agagagcgcc cgtgcgcaga
    13861 ccagcctcaa cgtgaccaac aggaatacgc tgataaaacg ccgccaggct ggttgccagc
    13921 gtcgtcgtcg tatcgccgtg aaccagcacg acgtctggtt tgaactcggc aagaataggt
    13981 tttagccctt ccagaatccg acaggttatc tctgtcaggc cctgtcctgg ctgcattatg
    14041 ttgagatcgt agtcaggtac aatggaaaag agtttcagta cctgatcaag catctcccga
    14101 tgctgcgcag tgacgcaaac tttagcctca aaaaaaggat cttttgccaa cgcatgcacc
    14161 aacggagcca tcttgatggc ttccgggcgc gtaccaaata cagtcagtac tttcacatcg
    14221 attctcttcg aataagcggc gagcgccttt gcgctcaccg cagcagtgtt gctatttcga
    14281 gcaacggcgg gttaatgcga caccagcccc gatcagcccc ccgacaatgc cccacataat
    14341 catcaggaag gcacgacgtg ggctatcgcg ttttaccggt tcttccggcg tacgcaaata
    14401 gcgataggtc tgaaaacgcg gatccagggt tggaccaaca ttcagggtgt ttaacatggc
    14461 tcgattctga tcatagtcga gatcaaaggc cggaccgacg gcctgtaaat tttccagtcg
    14521 agcctggagc attggacgcc caagcaggaa catttctgaa tcaggtaatt cctcggcagg
    14581 cacatctgtc gcactgcgcg aaatattatg ctgctcagca attttcagcg cctgctcaat
    14641 actgttcatc cggcggtcgt agatggcttt cgccacctct tcctgacgct tcacctgagc
    14701 tttcatctgg atggtgcgtg ccgcccatgc gcctttcagc tcatcattca gatggctggc
    14761 tgcacgctgg ctggcaaaag caacatactg acgtaacagg ttattagcgt caggcgcggt
    14821 ttcggcaata agcttcacgc tgtcattgac cgcgcgagta aagtctccgg ggataaactg
    14881 gatgttgtta atcatttcat ccagcaacgc cgcatcggct ttgctgttgc ccaccatccg
    14941 ctgtttgtaa tagtcggttt gcagccagaa ctctctgcgg gtatcccacg aggccagttg
    15001 cataacaaac tctttatagg cttcgtccat gaccgatggt tgatcggcag aagccatgtt
    15061 tgaacggaca tccaggttac gcaaaaattg ctgctgcgag taatatcccc ccagcatatt
    15121 caccgttgga cgatcggtaa tcgccgtcga gctccactcc tgacgagcaa aaaaagtata
    15181 cgccagcgcg attaacgcaa acgccagccc catgccaata atccatagct tcccagccca
    15241 caaggtacga aacaacccac gaatatccag ttcattttca gcgtcttcgg ccggtttccc
    15301 aggcattggt tgtgtcatca catcctcatt tatttggtta aattggggct gccaccacga
    15361 tttctacgca gtctgcgttt tacgcgctta ataaagcgag caactttcca ggcacgctta
    15421 atgcaatatc catagaggaa gaatgctagc aaaaagagca ccagcatgac ccactccggg
    15481 acaaaatgag aatattctgc cagcacgcca atggaagcga gcagtgctgc ggcaagggta
    15541 atcagcacaa acgcctgacg ggaagtaaac ccggcacgca tgatcaaatg gtgaatatgc
    15601 tgacggtcag gagagaatgg gctcatgcct ttacgcaggc gacggtacat aatcgccacc
    15661 atatccatta gcggaatggc gattatccac aaagcggtaa ccgggctgat gggatgggtt
    15721 ttgccctggg tcgtttcgag caggatccag ataacggtaa aaccaatcag cgtactgccc
    15781 gcatcaccca taaagacttt gtagcggcga cccaggatac caaggttaag catgatgtat
    15841 ggcaggatgg cggcgatcat cgcaaagcac cagattgcga ggctggtttg cccgtcgaac
    15901 cacaaaatca taccgattgc tgcaaacgag acgcaggaca acccgcccag caagccatca
    15961 atgccatcaa ccatgttgaa cgcattaatg gccgcccaga cggcaaatag cgtcaggaag
    16021 taaccaaacg gtccgagcac catctcccag gagccaaaga tataacccag gctactgaga
    16081 taaagtttgc cgaacaccat cataacaatg ccaacagcgg cctgtatggt ggcacggatt
    16141 tttacgctga tatcaaaacg atcatccagc gcgccaatga aaacaagcac accggcacaa
    16201 gcgagataga gagatgcatg cggaatatag taatcgacaa ttccgaacgt gaagcaaatc
    16261 cctgcgtaaa ccgaaatccc cccaacgaga ggtatcaatc cctggtgacg tttgcggaag
    16321 tttggtttat ccactaaacc gacttttttt gccaccttac gggcaaaaaa cagaaacagt
    16381 gtcgtgaata aaaaaatact gatgagatca gtactcactg tcagtaaatt cacaatgcat
    16441 gctctcagag aaaattatta gcagaagtat aaccacgaag acctttattc agaagggaaa
    16501 gtccgttccg aacccaccag ccctgtgcaa aaatcaacca tttgatatgc aaattgcata
    16561 acatgccagc aaattccagg aattttccta acatcgccag cgcgacatta agattacaga
    16621 tataaaagca aaacgccacg taaacacgtg gcgtttttgg cataagacaa atttatgagc
    16681 gtttcatcat ttcgaagaaa tcgtcattgg tcttggtcat ggccagttta ttaatgagga
    16741 attccattgc atcgatttcg cccatcgggt gaatgatttt gcgcaggatc cacattttct
    16801 gcagttcttc ctgagtcgtg agcagctctt ctttacgggt accggaacgg ttgtagtcga
    16861 tagccgggaa gacgcgtttt tcagcgatct tacgagagag gtgcagttcc atgttgcctg
    16921 tacctttaaa ctcttcgtaa ataacttcgt ccattttaga accggtatcg ataagcgccg
    16981 tcgcgataat ggtcaggctg ccgccctctt ccacgttacg cgccgcacca aagaagcgtt
    17041 tcggacgatg aagggcattg gcgtccacac caccggtcaa cactttacct gacgccggaa
    17101 caacagtgtt gtaagcgcgc gccagacgag tgatggagtc gagcagaatg ataacgtctt
    17161 tcttgtgctc aaccaggcgt ttggccttct cgatcaccat ttccgcaacc tgaacgtggc
    17221 gagatgcggg ttcgtcaaag gtagaagcaa caacttcacc ttttaccaga cgctgcatct
    17281 cggttacttc ttccggacgt tcgtcgatca gcagaaccat cagcacacaa tccgggtggt
    17341 tgtaagcaat gctctgagca atgttctgca gcagcatagt tttaccggct ttcggcggtg
    17401 ccacaatcag accacgctga ccacgaccga taggtgatgc cagatccagt acgcgagcag
    17461 ttaaatcttc agtagaaccg ttaccacgtt ccatacgcag acgagagttt gcgtgcagcg
    17521 gggttaagtt ctcaaagagg attttgttgc gggcgttttc aggtttgtcg aagttaactt
    17581 cgttaacttt cagcagcgca aaatagcgtt caccttcttt cggcgggcga atcttaccag
    17641 agatggtatc accagtgcgg aggttgaaac ggcggatttg gctaggggaa acgtagatgt
    17701 catcaggacc ggcgaggtag gagctgtctg cggaacggag gaaaccaaat ccatcctgca
    17761 atatctccag tacgccatca ccaaagatat cttcgccact ctttgcgtgc tgcttcagga
    17821 tggcaaaaat aatgtcctgc ttacgcatgc gagccaggtt ttccagcccc atattttcgc
    17881 cgagagtgat cagctcagaa accggcgtat tctttaattc ggtaagattc atagtggtgt
    17941 gagttcttaa acttggggta aatctcgaac ttaatgttgt gaatggtatg gcaggatcat
    18001 ccatgcctgt ttacggtcat cgactcatgt cttttcgctg cctggtcaca ggagagtacg
    18061 cagaactgaa acgacaagac ggattgagtg acgagccaga aatctgttca cttcgcattt
    18121 aagataaaac gggaagcgtt ggaaattaca agattcaaac ttaataaggt atgtttaata
    18181 cgaagtcaac actaagttag catgactcac gccgggcgtc cagtttttag cgacggggca
    18241 cccgaacatg aaattccctt acgccaggtt agcgtcgagg aactctttca actgaccttt
    18301 agacagtgcg cccactttgg ttgccgccac ttcaccgttt ttgaacagca gcagagtcgg
    18361 gataccacgg atgccatatt tcggcgcagt gccagggttt tgatcgatgt tcagttttgc
    18421 aacggtcagt ttgccctgat attcgtcagc gatttcatcc agaatcgggg cgatcatttt
    18481 gcacggaccg caccactctg cccagaaatc gacgaggatc gccccgtccg ctttgagtac
    18541 atccgtgtca aaactgtcgt cagtcaggtg aataatttta tcgctcatat ataactccac
    18601 aggaataagc ctggcgtgtt ggtttcgttg ttggtgtaac attaaccaac taaaggttga
    18661 ctttatttca ccggatacgc tttcgtaaag caatagtaag ctgatattct accacactat
    18721 gagcaaaaca catttaacag aacagaagtt ttccgacttc gccctgcatc cgaaggttgt
    18781 agaagccctt gaaaaaaaag ggtttcataa ctgtacgccc attcaggcac tggcccttcc
    18841 gctgacgctg gcgggtcgtg acgtagccgg gcaggcgcaa accggtaccg ggaaaacgat
    18901 ggcgtttctt acgtcaacgt ttcattatct tctctctcat cctgcgattg ccgatcgcaa
    18961 ggtgaatcaa ccgcgtgcct taattatggc accgacgcgt gaacttgccg tgcagatcca
    19021 cgccgacgcg gaaccgctgg cggaagctac cggcctgaag ctgggcctgg cttacggtgg
    19081 tgatggctat gacaaacagc tgaaagtgct ggaaagcggc gttgacattc tgattggcac
    19141 cacgggtcgt ttaattgact acgccaagca gaaccacatt aacctcggtg ccattcaggt
    19201 agtggtactg gacgaagccg atcgcatgta cgatctaggc tttattaaag atatccgctg
    19261 gctgttccgc cgtatgccgc ctgcaaatca gcgcctcaac atgctgttct ccgccacgct
    19321 ttcgtaccgg gtacgtgaac tggcgttcga gcagatgaac aatgccgaat atattgaagt
    19381 ggaaccggaa cagaaaacgg gccaccgtat taaagaagag cttttctacc cttctaacga
    19441 agaaaaaatg cgtttgctgc aaacgctgat cgaagaagag tggccagaca gagcgattat
    19501 tttcgccaat accaaacacc gttgtgaaga gatctggggc cacctggcgg cagatggtca
    19561 tcgtgtcggt ttgttgacag gcgatgtcgc gcagaaaaaa cgtctgcgta ttcttgatga
    19621 atttacccgt ggcgatctgg atattctggt tgccaccgac gttgccgcgc gtggtttgca
    19681 tattccggca gtgacgcacg tctttaacta cgatttaccc gatgactgtg aagattacgt
    19741 tcaccgtatt ggtcgtacag gtcgcgcagg cgcaagcggt cactctatca gcctggcgtg
    19801 tgaagagtat gcattgaatt tgcctgctat tgagacctat attggtcact caattccggt
    19861 aagcaaatac aatccggacg cattgatgac cgatctgcca aaaccgctgc gcctcacgcg
    19921 cccgcgcaca ggcaatggtc cgcgtcgtac tggcgctccg cgtaatcgtc gtcgttcagg
    19981 ttaaataaaa tgtcatactc aaaatcattc aaactgtatc aagatggcaa gagaatgaat
    20041 cccgatgagc ttactgtagt aagtgattcg ggtgaaagaa tgcagccaac acagagacag
    20101 attgaaggat gaagagtatg ggttccacct cgtcgctgta tgcagccatt gatctcggtt
    20161 cgaatagttt tcatatgctg gttgtgcgcg aggtggctgg aagcatccag acgctgacgc
    20221 gaattaaacg caaagtgcgt ctggctgctg gcctgaatag cgaaaatgac ctctctaatg
    20281 aagcaatgga gcgcggttgg caatgtctgc gcctgtttgc tgaacgtctg caagatatcc
    20341 ctccctcgca aattcgcgtt gtcgctacgg cgacattacg acttgccgtc aatgcgggtg
    20401 attttattgc caaagcgcag gaaatcctcg gttgtccggt acaggtgatc agcggtgaag
    20461 aggaagcgcg tctgatttat cagggcgttg ctcacaccac tggtggtgcc gatcagcgcc
    20521 tggtggtgga tataggcggt gccagtactg aactggtaac cggcacgggt gcacaaacca
    20581 cctcgttgtt cagcctgtcg atgggctgcg tcacctggct ggaacgctat tttgccgatc
    20641 gtaatctggg gcaagaaaat tttgatgctg cagaaaaagc ggcacgcgaa gtgttacgtc
    20701 cggttgccga tgaattacgg tatcacggct ggaaagtgtg cgttggtgct tccggcaccg
    20761 tgcaggcgtt acaggaaatc atgatggcac aggggatgga tgaacgcatt accctggaaa
    20821 agttgcagca attgaaacag cgagccattc attgcggtcg gctggaggaa ctggagattg
    20881 acgggctgac gctggaacgt gcattagtgt tcccgagtgg tctggcgatc ctgatcgcca
    20941 tttttaccga actgaatatt cagtgtatga ccctggcggg cggtgcgctg cgtgaaggcc
    21001 tggtctacgg catgttgcat ctggccgtcg agcaggatat tcgcagccgt acactgcgta
    21061 atattcaacg ccgctttatg atcgatattg atcaggcaca gcgcgtagcc aaagttgcgg
    21121 ctaacttctt cgatcaggtg gaaaacgaat ggcatcttga agcaataagc cgcgatttgc
    21181 tcatcagcgc ctgtcagctt catgaaatcg gcctgagcgt tgacttcaaa caagctccgc
    21241 aacacgctgc ttatctggtg cgtaacctgg atcttcccgg ttttaccccc gcacagaaga
    21301 aactgctggc gacgctactg ctcaaccaga ctaacccggt cgatctctca tcgctgcatc
    21361 agcaaaatgc cgtaccaccg cgcgtcgcag aacaactctg ccgtttactc cgcctggcta
    21421 tcatttttgc cagccgtcgc cgtgacgatc tcgtgccaga gatgacatta caggctaacc
    21481 atgaactgtt gaccttgacg cttccgcaag gttggctaac ccaacatccg ctgggtaaag
    21541 agattattgc tcaggaaaac cagtggcaga gctatgtcca ctggccgctg gaagtgcatt
    21601 aatgagtaag tgccggatgc gatgctgacg catcttctcc ggccttgatt atttccctcg
    21661 ttttgccgcc atcatcgctt tcagattcgc cagatggctt tgccctttct gcatccgttc
    21721 ttcggcactg accactttgc gctcctgttc ccaaatcaga tcatcctgcg gcagctccag
    21781 caaaaagcgg ctcggctccg ggcgcaccag ttcgccgtac tgacggcgtt ctttacacag
    21841 cgtaaaggtc aattccttct gggcgcgggt aatgccgaca taggccagcc gccgctcctc
    21901 atcgatatta tcttcatcga tactgctctg gtgcggcaaa aacccttctt ccataccgac
    21961 catgtagaca taaggaaact ccagcccttt tgacgcgtgg agagtcatca gttgcacctg
    22021 atccagctct tcttcactct caccacgctc catcatgtcg cgtaaagtaa agcgcgtcac
    22081 cacctgggtg agcgtcatcg gctcatccag ttcgctgcct tccagcatct ccgtcatcca
    22141 gctaaacagt tggttgacgt tcttcatgcg catttcggca gctttcgtgc tgggcgatgt
    22201 ttcgtacagc caggattcat aatccatgcc gtggatcaga tcgcgcaccg cggcaatcgg
    22261 ctcccgctcc gccagacgct ggatttctgc caaccagtga gtgaagcggg tcaatgcttc
    22321 ataaccacgt ccgctaagcg tctgactcag gcccatatca aagctggcgg taaacatgct
    22381 tttattgcgc gtcatcgccc actcacccag ctttttcagc gtcgccgggc caatctctcg
    22441 cttcggcgtg ttaacgatac gcagaaatgc gctgtcatcg tccgggttag tcagcacgcg
    22501 cagataagcc agcaagtcct tgatttcagg acgagagaaa aacgacgtac caccagaaat
    22561 tttgtacggg atgcggtttt gcatcaggaa tttttcaaac acccgcgact gatagttacc
    22621 gcgataaaga atagcgtaat ctttgtactg cgttttattg acgaagtgat gcgcaatcag
    22681 ttcaccagtg acgcgctcgg cttcatgttc ttcgttattc gcgcttaaaa cttttagctc
    22741 tgtgccgtaa cccagttcgg agaacagacg cttttcaaag acgtgcgggt tattggcgat
    22801 caggatgttc gccgctttca aaatacgtcc ggaagagcgg tagttctgct caagcttaat
    22861 caccttcagc gccggaaaat cctggctcag cagcaccagg ttttgcggac gtgcgccgcg
    22921 ccaggagtag atcgactggt catcgtcacc caccacggta aagcgcgcgc ggctgcccac
    22981 cagcagtttc accagttcat actggctggt gttggtgtcc tgatattcgt ccaccagcag
    23041 gtagcgaatt ttgttctgcc agcgctcgcg gacttcttca ttgcgttgca gaagtagcgt
    23101 cggcaataaa atcagatcat cgaagtcgag cacattacag gctttcaggt gtgcatcata
    23161 cagcccataa caatgggcaa aaatacggtc ccgctcgcca atcgcactgg ctgccgcctg
    23221 ggacggtgtt ttgagatcgt tcttccagtt agagatggtc gaaatcaact gttgcaggag
    23281 aactttgtca tcttcaatca gcccttcggt caactctttg agcaacgcca gctgatcggt
    23341 atcgtcaaac agcgaaaagt tcgccttcat ccccagcgcc gcatactcac gcttgatgat
    23401 atccagcccc agcgtgtgga aggttgagat catcagccca tgcgcctctt tgcgacccag
    23461 cgtctgtccg acacgctctt tcatctcgcg cgccgcctta ttggtaaagg tcaccgccgc
    23521 gatatgtcgc gcctgataac cgcacccgcg gatcagatgg gcgattttat tggtgataac
    23581 acgagtttta ccggaacccg cacccgccag caccaggcag gggccggtaa cgaattcgac
    23641 agcttgttgt tggccggggt ttagacgcat aggaaaatcg ctcaatgaaa gtcgggaggg
    23701 gaaaataaca gcgtggtagt atagccagcc tcatccttac cactcaaggc acgatcatgg
    23761 caaaaacagc agcagcactg catatccttg taaaagaaga gaaactggct ctggatcttc
    23821 tcgtgcagat taagaacggt gccgatttcg gcaagctggc gaagaaacac tccatttgcc
    23881 catcaggcaa acgcggcggt gatttaggtg aattccgcca gggtcagatg gttccggcgt
    23941 tcgataaagt tgtgttctct tgtccggtac tggagccaac cggcccgctg cacacccagt
    24001 tcggttacca catcattaag gtgctgtacc gtaactaata gcaaggcctt ctccgggaga
    24061 aggccttgaa ctctgaatcg ctattaaccc gcaacagcaa tacgtttcat atccgtcata
    24121 tagccgcgca gtttcttacc tacttgctca atcgcatggc tgcgaatcgc ttcatttaca
    24181 tcacgcagct gcgcgttatc taccgcacct tccggaatag ctttacccaa atcgcccggt
    24241 tgcagttctg ccataaacgg tttcagcagc ggcacacaag cgtaagagaa cagatagtta
    24301 ccgtactcgg cagtatcaga gataaccacg ttcatttcat acagacgctt acggacgatg
    24361 gtgttggcaa tcagcggcag ttcgtgcagt gattcatagt aagcagattc ttcgatgatg
    24421 ccggaatcaa ccatggtttc gaacgccagt tcaacgcccg ctttcaccat cgcgatcatc
    24481 agtacgcctt tatcgaagta ctcctgctcg ccgattttgc cttcatactg cggcgcggtt
    24541 tcgaaggcgg ttttgccggt ctcttcacgc caggtcagca gtttcttatc atcgttggcc
    24601 cagtccgcca tcataccgga ggagaattcg ccggagatga tgtcgtccat atgtttctgg
    24661 aacagcggtg ccatgatctc tttcagctgt tcagacaacg cgtaagcacg cagtttcgcc
    24721 gggttagaga gacggtccat catcagtgtg ataccgccct gcttcagcgc ttcggtgatg
    24781 gtttcccaac cgaactgaat tagtttttct gcgtatgccg gatcggtgcc ctcttccacc
    24841 agcttgtcga agcacagcag cgagccagcc tgcaacatac cgcacaggat ggtttgctca
    24901 cccatcaggt cagatttcac ttccgcaacg aaggacgatt ccagcacgcc cgcacggtga
    24961 ccaccggttg ctgccgccca tgctttagcg atcgccatgc cttcgccttt cggatcgttt
    25021 tccgggtgaa cggcaatcag cgtcggtacg ccgaatccac gtttgtactc ttcacgcact
    25081 tcggtgccag ggcatttcgg cgcaaccatg actacggtga tgtctttacg gatctgctcg
    25141 cccacttcga cgatgttgaa accgtgcgag taacccagcg ccgcgccgtc tttcatcagt
    25201 ggctgtacgg tgcgcactac atcagagtgc tgcttgtccg gcgtcaggtt aaccaccaga
    25261 tccgcctgtg ggatcagttc ttcgtaagta cccactttaa aaccattttc ggtcgcttta
    25321 cgccaggacg cgcgcttctc ggcaatcgct tctttacgca gagcgtagga gatatcgaga
    25381 ccagaatcac gcatgttcag accctggttc agaccttgtg cgccacagcc gacgatgact
    25441 acttttttac cctgaaggta gctcgcgcca tcggcgaatt catcgcgccc cataaagcga
    25501 catttgccca gctgtgccag ctgctgacgc agattcagtg tattgaagta gttagccatg
    25561 gtgattcctc gtgatgttgt gcttcttatt gttcggttcg cgtttgcgag attgaattca
    25621 ctatatgaca ggaaatttat tgcggaaatt gatatattca caacgtcaca ttgcaatttc
    25681 tgcaacgtca acattgaggg ctgcgccatt ggatttacgc gatctgaaaa ccttcctgca
    25741 tctggcggaa agccgccatt ttggccgcag cgcgcgggcg atgcacgtta gcccgtccac
    25801 gctctcacgg cagattcagc gcctggaaga agatctcggc cagccgctgt ttgtgcgcga
    25861 taaccgcacg gtgacgctga ctgaagcggg cgaagaactg cgcgttttcg cccagcaaac
    25921 gctgttgcag tatcagcagc tgcgccacac catcgaccag caagggccgt tgctctatgg
    25981 cgaattacat atcttctgct cggtgaccgc cgcttacagc catctgccgc cgattctgga
    26041 tcgcttccgc gcggaacacc cgtcggtgga gatcaaactc actaccggtg atgcggcaga
    26101 tgcaatggaa aaagtcgtca caggtgaagc tgatctggcg attgccggga agccagagac
    26161 tctgcccggc gcggtggcgt tttcgatgtt ggagaatctg gcagtggtgt tgattgcacc
    26221 cgcattgccc tgcccggtgc gtaatcaggt ttctgtcgaa aagccggact ggtcaacggt
    26281 accgtttatc atggccaatc aggggccggt gcgccgccgc attgagttgt ggttccgacg
    26341 caataaaatc agtaacccga tgatttacgc cacggttggc gggcatgaag cgatggtatc
    26401 gatggtggcg ctcggctgtg gcgtggcatt gttgccggaa gtggtgctgg aaaacagccc
    26461 cgaaccggtg cgtaaccgcg tgatgatttt agagcgcagc gatgagaaaa cgccgtttga
    26521 gctgggcgtg tgtgcgcaga aaaagcggct acatgagccg ctaattgagg cattctggaa
    26581 gattttgccg aaccattaac ccgccaaaaa gaacctgaac gccgggttat tggtttcgtc
    26641 gtggcaatcg tagcccagct cattcagccg ggtttcgaaa tccggttcat ggtcgccaag
    26701 ttcgaacgcc gccagtacgc gcccgtagtc ggtgccatgg ctgcgatagt ggaacaaaga
    26761 aatgttccag tgcgtaccca gcgtgttgag gaagcgcagc agcgcgcccg gtgattccgg
    26821 gaattcgaag ctgtagaggc gttcctgcaa cggatgtgac ggacgcccgc cgaccatata
    26881 gcgcacatgc aacttcgcca tttcgtcgtc ggagagatcc accacgctgt agccgccgtc
    26941 gttgagcatc tgcaaaattt ctttgcgctc ttcgaggccg cggctcaggc gcacgccgac
    27001 aaagatgcag gcgtttttgg catcggcaaa acggtagttg aactcggtga ccgatcgccc
    27061 gccaagcagt tggcagaatt tgaggaagct gcctttttct tccggaatgg tcaccgccaa
    27121 caacgcttca cgctgttcgc ccagttcgca gcgttccgag acgtagcgca ggccgtggaa
    27181 gttcacgttg gcaccggaaa gaatatgcgc cagccgttcg ccgcgaatgt tgtgctgggc
    27241 gatatatttt ttcattcccg ccagcgccag cgcgccagag ggttccgcca ccgcacgcac
    27301 atcttcgaat aaatccttca tcgccgcaca gatcgcatcg ctatcgacgg tgatgatgtc
    27361 gtcgagatac tcctggcata aacggaaggt ttcgtcaccg atgcgtttta ccgcgacacc
    27421 ttcggcgaac agtcccacgc gtggcagatc aaccggatga cccgcatcca gcgctgcttt
    27481 caggcaggcg gagtcttccg cttctacggc gatcactttg atttgcggca tcagttgttt
    27541 gatcagcacc gctacgcccg ccgccagacc gccgccgccg actggcacaa atacgcggtc
    27601 gagatgggcg tcctgctgga gcagttccag cgccagcgtg ccttgcccgg caatcaccat
    27661 cggatggtcg aacggcggca cccaggtgaa cccctgctgc tgtgacagtt cgatcgcttt
    27721 agctttcgct tcgtcaaagt tcgcgccatg aagcagcact tcgccgccga atccacgtac
    27781 cgcatcgact ttgatgtcgg cggtggcggt tggcataacg atcagggcct tcacgcctaa
    27841 ccgcgcagaa gaaaacgcga cgccctgcgc gtggttaccc gcagaagcag tgatcacgcc
    27901 gtgcgctttt tgttcttccg tcaggcccgc catcatggcg tatgcgccgc gcagcttaaa
    27961 gctgtgcact ggctggcgat cttcgcgctt caccagaatg acgttatcaa gacgcgacga
    28021 cagtttttcc attttttgta gcggcgtaac ctgcgccgcc tcgtaaaccg gcgcgcgcag
    28081 cactgctctt aaatattcgg caccttccgg agcaccggac aggggttgcg agtcagccat
    28141 tattaacccc ccagtttcga tttatcgcgc accgcacctt tgtcggcgct ggttgccagg
    28201 ctggcgtagg cgcacagggc aaaggaaacc tgacgttcac ggtttttcgg cgtccaggct
    28261 ttgtcacccc gagcttcctg cgcttcacga cgcgccgcca gttcggcatc gcttacctgt
    28321 aactgaatgc cacggttcgg gatgtcgata gcgatcaggt caccatcttc aatcagacca
    28381 atgctgccgc cgcttgccgc ttccggtgag acgtggccga tggaaagacc agaggtgccg
    28441 ccagagaaac gaccgtcggt gatcagcgca caggctttac cgagacccat tgatttcagg
    28501 aagctggttg ggtagagcat ttcctgcatg ccagggccgc ctttcgggcc ttcatagcgg
    28561 atcaccacca catcgcccgc gacaactttg ccaccgagga tcgcttctac cgcgtcgtcc
    28621 tggctttcgt acactttcgc cgggccggtg aatttgagga tgctgtcatc gacgcccgcg
    28681 gttttcacga tgcagccgtt ttcagcaaaa ttaccgtaga gcaccgccag gccgccgtct
    28741 ttgctgtagg cgtgttccag cgagcggata cagccgtttg cgcgatcgtc atcgagagaa
    28801 tcccaacggc aatcctgcga gaatgcctgt gtagtacgaa tgcccgcagg gcctgcgcgg
    28861 aacatatttt ttaccgcgtc atcctgggtc agcataacgt cgtattgttc cagagtttgc
    28921 ggcaacgtca ggccaagtac gtttttcaca tcacggttca gtaaccccgc gcgatccagt
    28981 tcaccgagaa taccgataac accaccagca cggtgaacat cttccatatg gtatttttgg
    29041 gtgctcggcg caactttgca taactgcggg actttacgcg aaagtttgtc gatatcactc
    29101 atggtgaagt cgatttccgc ttcctgcgcc gccgccagca ggtgaagtac cgtgttggta
    29161 gagccgccca tcgcgatatc cagcgtcatg gcattttcaa acgccgcttt gctagcaata
    29221 ttgcgcggca gtgcactttc gtcgttttgc tcgtagtaac gtttggtcaa ttcaacaatg
    29281 cgtttaccgg cattgaggaa caactgctta cggtccgcgt gggttgccag cagcgagccg
    29341 ttgcccggct gcgacaggcc cagcgcttcg gtcaggcagt tcattgagtt agcggtaaac
    29401 atcccggagc aggagccgca ggtcggacac gcggaacgtt caacctgatc gctctgggag
    29461 tcagaaactt tcgggtctgc gccctggatc atcgcatcaa ccagatcgag tttgattatc
    29521 tgatcggaca gtttggtttt cccggcctcc atcgggccgc cggaaacaaa gatcaccgga
    29581 atattcaggc gcagggaagc catcagcatc cccggggtga ttttgtcgca gttagagatg
    29641 cagaccatgg cgtcggcgca gtgggcgttg accatatact caacggaatc agcgatcagt
    29701 tcgcgagatg gaagtgaata aagcatcccc ccgtggccca tggcaatccc atcatccacc
    29761 gcaatggtgt tgaactcttt ggcaacgccg ccagccgctt caatttgttc ggcgaccagt
    29821 ttaccgagat cgcgcagatg gacgtgaccc ggtacaaatt gggtgaacga gttcacaacc
    29881 gcgataatcg gcttgccaaa atcggcgtcg gtcattccgg tggcgcgcca cagcgcacga
    29941 gcacccgcca tattacgacc atgagtggtg gtggcggaac ggtacttagg catactttat
    30001 ttactcccag tgtctgtctc gtaaatggga cggtgcgtgc cgtcccattt tttgtattta
    30061 ttgattaact tgatctaacc agccccattt atcttcggtt tcgccagtga agaggccgaa
    30121 gaaggcttgc tgaatgcgtt tggtaaccgg gccacaacgg ccttcgccaa tctgaatacc
    30181 gtctacgctg cgcactggcg tgatttctgc cgccgtaccg gacataaaga cttcatccgc
    30241 caagtacagg gattcgcgcg acagcacctg ctcacgcact tcaattccca gctctttcgc
    30301 cagtttgatg atggcatcac gggtaatacc cggcagcgcg gaggaggtga acggtggggt
    30361 gaacagcaca ccatctttca cttcaaacag gttttcgcct gcgccttcag agatataacc
    30421 gttcacatcc agcgcgatac cttcctgata accgtggcgg cgcgcttcgc tacccaccag
    30481 cagggaagag agatagttac caccggcttt tgccgcagtt gggatggtgt ttggtgctgc
    30541 gcggttccag gaggaaacca tcgcatcgat cccctgctcc agcgcttctg cgcccagata
    30601 cgctccccac gggaaagcgg cgataatcac atcggttgag tatcccgctg gcgggtttac
    30661 tcccatgcca acatcaccga cgaagatcag cggacggata taggcgctgg tgagattgtt
    30721 tttgcggatc acgtcacgac aagcttccat cagctcatca atgctctgtg aaaccgggaa
    30781 gcgatagatt ttggcggagt catgcagacg ctgcatatgc tcacgatggc ggaatacaac
    30841 cggtcctttg tgtgagtcgt agcaacggat gccttcaaaa accgaggtgc catagtgcag
    30901 cgcgtgcgac atcacatgca ccttcgcgtc ttcccagcga accatctccc cattgaacca
    30961 aatgtaatca gctttcttcg tggtcatttt tatattcctt ttgcgctcag gcgcggattt
    31021 gttgtgatgt ggttgtgctc tggcagatgg caacgtgtgc gacgtccacc agtttattta
    31081 actgactaaa cagtaagtcg accgaccgtg ggctggcaac ggtcaattcg atatttatat
    31141 tttgtgcatc gctggcggca gccatattca ttgagcagac gtggaaacca cgatgacgca
    31201 ccacgcgtaa aacacgttct aaggtttccg gattgaagcg agccgataca ttgacctgat
    31261 gttgcatcat gataatttct ccaacatttc tgaattactg gcacctggcg gcaccagcgg
    31321 ccagacgttc tcaagttcgt cgattgagac atgaagcagg tatggcccat cactgttcag
    31381 catggtgtcg agtgccgctt caacctggtc tttacgggtg atgtgttggc cagggatgcc
    31441 gaaggcgctg gctaacatga ggaaatcggg gttatcggta agggtggttt cgctgtatcg
    31501 ttcctgaaaa aacagttgct gccattgtcg aaccatccct aaccgttggt tatcgagtaa
    31561 gacgattttc aacggtaact gcttgcgttt tacggtgccc agctcttgca cattcatcat
    31621 gaaagagccg tcaccggaga tacagacaac ggtatcgttc ggtcgcgcga cttgtgcgcc
    31681 aactgccgcc ggtaaaccaa aacccatggt acctaagccg ctggaggtga tgaaattttc
    31741 cgggcgagtg tgggcgatgt gctgcgcagc ccacatctgg tgctgcccca catctgtggt
    31801 cacgacgcaa tccgcaggtt tacgatccga cagttgtttt aacaacaacg gagcgtagat
    31861 agcgtcaccg ggatggtcgt aacgccagga atgttcatca cgcagctgcg cgcagtgttg
    31921 ctgccagtca ttgatattta acggctgctg taatgctggt aacagagcat ttaaatcacc
    31981 ttgtaatgcc acatgtgcct gacgcagctt gttcatttct gccgggtcga tatccatatg
    32041 gataacactg gcgtgtggtg cgaaggtgtt cagtttgccg gtcacccggt catcaaaacg
    32101 tgcacccacg gcgatcagca ggtcacactc ctgcaccgcg aagtttgccg ctttggttcc
    32161 gtgcatcccc agcatgccca gatagtacgg ataatctgct tctactgcgc ccagcccttt
    32221 cagcgtacag gtggcaggca tttttgtggc agcgagaaat tcacgcaaag ccggaactgc
    32281 ctgcgccatc cccacgccac cgccaacgta cagcatcggt ttttgcgctt ttgccagcat
    32341 ctggcgcgct tgctcgactt cggcatgtgg gaaagtcact tcgttttcaa cggtggtgaa
    32401 ccacggttcc agatcgccgc tggctaactg gatatctttt gggatatcga ccagaaccgg
    32461 accaggacga cctgagctgg caacgtcgaa tgcttcagcc atgatgcgcg gcaactcttc
    32521 cagagactgc accagaaagc tgtgcttggt acaggctaac gacaatccca ggacatccac
    32581 ttcctgaaat gcgtcggtgc cgataaacgg tgcggacacc tgaccagtga tggcaacgac
    32641 agggatggaa tctaacagtg cgtccgcaag cccggttatc aggttggttg cgcccggacc
    32701 agacgtggcg atacatacgc cagttttacc ggtagcacgg gcataaccga tagccgccat
    32761 tgccgcaccc tgctcatgtc ggcacagcaa gtgctccacg ccgccgtcat acaatgcatc
    32821 gtaaaccggc ataattgcgc cacccggata accgaaaacg gtgttcacac cctgtgcccg
    32881 caacgcatgt accacccact gtgcgccatt catagttagt tccccgtcct gaatcttgag
    32941 aaacagaatt ttgtgctgtt attcattgtc tgctcctcgg ttatgttttt aaggtcaaaa
    33001 aaacccccgg acctttcggt gcgggggtct tagttcgtta aggcttgatc tctaagcctt
    33061 tcctcgtcca agtgcagccc cgcacggtgg gataataatc accaccacgc taatcacgac
    33121 caggctaatc actcgtagaa gggctgtcat tttgtctttt cttgcatctt gttcgaagga
    33181 atgcctaaag agttaccata gattttgcga atagcacaag atatttttgg gcctattttt
    33241 atgatggctt taataatttc aatagaaatt gttgtttttt aaaaagaaaa tagtgagtga
    33301 aaaaattcaa tttcagtata aatgttgttc ccttcctcgt agttcatcct ttgtccgctg
    33361 catttttgcg agcatcattc caccgccaga acaactcaac ttcacttgca caaataaaca
    33421 ggaatcagcc actaaaattc acgaattagc gggctacagc agcgatatct tatgcacata
    33481 atggtgactc aaggagggtt tatgtcactg tcaattgttc atacccgcgc agccctggaa
    33541 gtaaatgcgc ccccaatcac tgttgaggta catatcagta aaggtctacc cggcttaacg
    33601 atggtgggct taacagaaac aacggtgaaa gaagctcgcg atcgcgtgcg cagcgccatt
    33661 atcaatagcg gatatgaata tccggcgaaa aaaatcacca tcaaccttgc tccagccgat
    33721 ctgccaaaag aagggggacg atatgattta cctatcgcca ttgcgttgct ggcggcctca
    33781 gaacagctta cagccaataa gttagatgaa tatgaattag tcggagaact agcgcttaca
    33841 ggcgctctgc gtggcgttcc cggtgcaatc tccagtgcaa ctgaagctat taagtcgggc
    33901 agaaaaatta tcgtcgcgaa agataacgaa gatgaagtgg ggctaattaa cggtgaagga
    33961 tgcctgatag ccgatcatct gcaggctgtc tgtgcgtttc tggaaggtaa gaacactctc
    34021 gaacgcccga aaccaactga tgcagtatcc cgggcgctac aacatgatct cagtgatgtt
    34081 gtcggtcagg agcaaggaaa acgaggactg gaaattaccg ccgctggtgg gcacaacctt
    34141 ttactgattg ggccgccggg aacaggtaaa acaatgctcg ccagccgtat taatggcctg
    34201 ttgccagatt taagcaatga agaggcactg gagagcgctg cgatattaag tctggtaaat
    34261 gctgaatcag tacaaaaaca atggcggcag cgcccgttcc gctcacctca tcacagtgcg
    34321 tcgttaactg cgatggtggg cggtggtgca attccagggc caggtgaaat ttcgttggcg
    34381 cataacggcg tgctttttct tgatgagcta cctgaatttg aacggcgtac actggatgcc
    34441 ttgcgagagc cgattgaatc cgggcagatc catctttcac gcacacgagc aaaaataacc
    34501 tatccagccc gtttccagct tgttgcggcg atgaatccca gccctaccgg acattatcag
    34561 ggaaaccata accgctgcac gccagaacag acattacgtt atctcactgg agatcccatt
    34621 accacccccc ggcattttga gtaaaacggt agtgccggga gaaagcagcg ccaccgttaa
    34681 acaacgcgta atggccgcca gagagcgcca atttaagcgg cagaataaac tgaatgcctg
    34741 gctggatagt ccggaaatac gccaattctg caaacttgag agcgaagatg cgatgtggtt
    34801 ggaagaaacg ctgatccatc tggggttatc gattcgtgcc tggcagcggt tactgaaagt
    34861 tgcacgaacc attgctgata ttgatcagtc tgacattatc acacgtcagc atttgcagga
    34921 ggcagttagc tatcgtgcga ttgaccgttt gctcatccat ctgcaaaaac tactgacata
    34981 aaaaagggca tttcgccctt tttattaatc gtcagaatcg gtgtagtctt cagcaccttc
    35041 aacctgcggt ttaccgccgg aaagggtgtg aaaacgtttt ggacgcttaa tacgcgtcat
    35101 atacttggac cacacgcgtt ctgcttctgt cactggctca cgttcgccac ggcatactgc
    35161 tacgaagagt ttctcttcct cggtaaccgg ctcgcgtttg ccaagatcca actcattgaa
    35221 ggcataacca tgacgctcaa gcagttgtgc ctctttgatg gtgaaatcac catgacgaga
    35281 gaatccacgt ggataatgtt tattgtcgaa atatcgatta gtcgtcgtaa agctttccgc
    35341 catcctgcac gctcctaatt ctttgaccga gctagttatg gcgcggagta ttagttacgc
    35401 ttgacagagt gtaaaacaaa acatttaaat cataacgaca aataattttg cggagagcac
    35461 tgtggatacg gaattgttaa aaactttcct ggaagttagc cgaacgcgtc actttggtcg
    35521 agcggctgaa tcgctctatc tgacccagtc agcagtgagc tttcgaatca gacaactgga
    35581 aaatcaactg ggtgtgaacc ttttcacccg ccacagaaac aatatccgtt taaccgctgc
    35641 cggtgaaaaa ctactgcctt atgcagaaac gctcatgagc acctggcagg ccgcccgcaa
    35701 ggaggtggcg catacctcac gacataacga gttttctatc ggtgccagcg cctcgttgtg
    35761 ggaatgtatg cttaatcagt ggttgggacg cttgtatcaa aatcaggatg cccatacagg
    35821 cttacagttc gaagcgcgaa ttgcccaacg gcagtctctg gtaaaacagc tgcatgaacg
    35881 ccagcttgat cttcttatta ccactgaagc gcccaaaatg gacgaattta gtagtcagtt
    35941 gctggggtat ttcactttag cgctttatac cagtgcccct tctaaactaa agggagatct
    36001 taactatctg cgactggagt gggggccaga ttttcaacag catgaggcag gtttgatcgg
    36061 tgctgacgaa gttcccattc tgacaaccag ttctgctgaa ctggcacagc aacagattgc
    36121 gatgcttaat ggttgcacct ggctacccgt cagctgggcg cgtaaaaaag gcggcctgca
    36181 taccgttgtc gatagcacaa cactttcacg gccgctttat gccatatggc tgcaaaatag
    36241 cgataaaaat gcgttgattc gcgatctttt gaaaattaac gtgctggatg aagtgtatta
    36301 atctgaatgg ctggcaagga tgccggtaga aggatttact tcggagagga ttatttcaga
    36361 taaaaaaaat ccttagcttt cgctaaggat gatttctggc aggggcggag agactcgaac
    36421 tcccaacacc cggttttgga gaccggtgct ctaccaattg aactacgccc ctaattaggg
    36481 tggcggaacg gacgggactc gaacccgcga ccccctgcgt gacaggcagg tattctaacc
    36541 gactgaacta ccgctccacc gaattctttt acaaccaccg gttttacgac cggctt
//
LOCUS       DAJTRC010000032        35761 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.032, whole genome shotgun sequence.
ACCESSION   DAJTRC010000032 DAJTRC010000000
VERSION     DAJTRC010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 35761)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 35761)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35761
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.032"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..247
                     /locus_tag="OO357_001714"
     CDS             <1..247
                     /locus_tag="OO357_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5886687.1"
                     /translation="VTIPRGQLKNLKASYTLTEPQLTAPLKKGQVVGTIDFQLNGKSI
                     EQRPLIVMENVEEGGFFGRMWDFVMMKFHQWFGSWFS"
     gene            complement(294..1052)
                     /gene="deoR"
                     /locus_tag="OO357_001715"
     CDS             complement(294..1052)
                     /gene="deoR"
                     /locus_tag="OO357_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional repressor DeoR"
                     /protein_id="HCR5886688.1"
                     /translation="METRREERIGQLLQELKRSDKLHLKDAAALLGVSEMTIRRDLNN
                     HSAPVVLLGGYIVLEPRSASHYLLSDQKSRLVEEKRRAAKLAATLVEPDQTLFFDCGT
                     TTPWIIEAIDNEIPFTAVCYSLNTFLALKEKPHCRAFLCGGEFHASNAIFKPIDFQQT
                     LNNFCPDIAFYSAAGVHVSKGATCFNLEELPVKHWAMSMAQKHVLVVDHSKFGKVRPA
                     RMGDLKRFDIVVSDCCPEDEYVKYAQTHRIKLMY"
     gene            complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="OO357_001716"
     CDS             complement(1110..1706)
                     /gene="ybjG"
                     /locus_tag="OO357_001716"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706720.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5886689.1"
                     /translation="MLENLNLSLFSLINATPDSAPWMISLAIFIAKDLITVVPLLAAV
                     LWLWGLTAQRQLVIKIAIALAVSLFVSWTMGHLFPHDRPFVENIGYNFLHHAADDSFP
                     SDHGTVIFTFALAFLCWHRLWSGSLLMVLAVVIAWSRVYLGVHWPLDMLGGLLAGMIG
                     CLSAQIIWQAMGHKLYQRLQSWYRVCFALPIRKGWVRD"
     gene            1991..3217
                     /gene="mdfA"
                     /locus_tag="OO357_001717"
     CDS             1991..3217
                     /gene="mdfA"
                     /locus_tag="OO357_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdfA"
                     /protein_id="HCR5886690.1"
                     /translation="MQNKLASGARLGRQALLFPLCLVLYEFSTYIGNDMIQPGMLAVV
                     EQYQAGIDWVPTSMTAYLAGGMFLQWLLGPLSDRIGRRPVMLAGVVWFIVTCLAILLA
                     QNIEQFTLLRFLQGISFCFIGAVGYAAIQESFEEAVCIKITALMANVALIAPLLGPLV
                     GAAWIHVLPWEGMFVLFAALAAISFFGLQRAMPETAMRIGEKLSLKELGRDYKLVLKN
                     GRFVAGALGFVSLPLLAWIAQSPIIIITGEQLSSYEYGLLQVPIFGALIAGNLLLARL
                     TSRRTVRSLIIMGGWPIMIGLLVAAAATVISSHAYLWMTAGLSIYAFGIGLANAGLVR
                     LTLFASDMSKGTVSAAMGMLQMLIFTVGIEISKHAWLNGGNGLFNLFNLVNGILWLSL
                     MVIFLKDKQMGNSHEG"
     gene            complement(3258..3542)
                     /locus_tag="OO357_001718"
     CDS             complement(3258..3542)
                     /locus_tag="OO357_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886691.1"
                     /translation="MIMKNCLLLGALLMGFTGVAMAQSVTVDVPSGYKVVVVPDSVSV
                     PQAVSVATVPQTVYVAPAPAPAYRPHPYVRHLASVGEGMVIEHQIDDHHH"
     gene            complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="OO357_001719"
     CDS             complement(3628..4443)
                     /gene="ybjI"
                     /locus_tag="OO357_001719"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase"
                     /protein_id="HCR5886692.1"
                     /translation="MSIKLIAVDMDGTFLSDQKTYNRERFMAQYQQMKAQGIRFVVAS
                     GNQYYQLISFFPEIANEIAFVAENGGWVMSEGKDVFNGELSKDAFATVVEHLLTRPEV
                     EIIACGKNSAYTLKKYDDAMKTVAEMYYHRLEYVDNFDNLEDIFFKFGLNLSDELIPQ
                     VQKALHEAIGDIMVPVHTGNGSIDLIIPGVHKANGLRQLQKLWGIDDSEVVVFGDGGN
                     DIEMLRQAGFSFAMENAGSAVVAAAKYRAGYNNREGVLDVIDKVLKHEAPFDQ"
     gene            complement(4443..5651)
                     /locus_tag="OO357_001720"
     CDS             complement(4443..5651)
                     /locus_tag="OO357_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5886693.1"
                     /translation="MTVNSSRNALKRRTWALFMFFFLPGLLMASWATRTPAIRDILSV
                     SIAEMGGVLFGLSIGSMSGILCSAWLVKRFGTRNVILVTMSCALIGMMILSLALWLTS
                     PLLFAVGLGVFGASFGSAEVAINVEGAAVEREMNKTVLPMMHGFYSLGTLAGAGVGMA
                     LTAFGVPATVHILLAALVGIAPIYIAIQAIPDGTGKNAADGTQHGEKGIPFYRDIQLL
                     LIGVVVLAMAFAEGSANDWLPLLMVDGHGFSPTSGSLIYAGFTLGMTVGRFTGGWFID
                     SYSRVAVVRASALMGALGIGLIIFVDSAWVAGVSVVLWGLGASLGFPLTISAASDTGP
                     DAPTRVSVVATTGYLAFLVGPPLLGYLGEHYGLRSAMLVVLALVILAAIVAKAVAKPD
                     TKTQTAMENS"
     gene            5735..6271
                     /gene="rcdA"
                     /locus_tag="OO357_001721"
     CDS             5735..6271
                     /gene="rcdA"
                     /locus_tag="OO357_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RcdA"
                     /protein_id="HCR5886694.1"
                     /translation="MRRANDPQRREKIIQATLEAVKLYGIHAVTHRKIATLAGVPLGS
                     MTYYFSGIDELLLEAFSSFTEIMSRQYQAFFSDVSDAQGACQAITDMIYSSQVATPDN
                     MELMYQLYALASRKPLLKTVMQNWMQRSQQTLEQWFEPGTARALDAFIEGMTLHFVTD
                     RKPLSREEILRMVERVAG"
     gene            complement(6446..8131)
                     /locus_tag="OO357_001722"
     CDS             complement(6446..8131)
                     /locus_tag="OO357_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001024888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate:alanine antiporter"
                     /protein_id="HCR5886695.1"
                     /translation="MNINVAELLNGNYILLLFVVLALGLCLGKLRLGSIQLGNSIGVL
                     VVSLLLGQQHFSINTDALNLGFMLFIFCVGVEAGPNFFSIFFRDGKNYLMLALVMVGS
                     ALVIALGLGKLFGWDIGLTAGMLAGSMTSTPVLVGAGDTLHHSGMESRQLSLALDNLS
                     LGYALTYLIGLVSLIVGARYLPKLQHQDLQTSAQQIARERGLDTDANRKVYLPVIRAY
                     RVGPELVAWTDGKNLRELGIYRQTGCYIERIRRNGILANPDGDAVLQMGDEIALVGYP
                     DAHARLDPSFRNGKEVFDRDLLDMRIVTEEVVVKNHNAVGKRLAQLKLTDHGCFLNRV
                     IRSQIEMPIDDNVVLNKGDVLQVSGDARRVKTIADRIGFISIHSQVTDLLAFCAFFVI
                     GLMIGMITFQFSTFSFGMGNAAGLLFAGIMLGFMRANHPTFGYIPQGALSMVKEFGLM
                     VFMAGVGLSAGSGINNGLGAIGGQMLIAGLIVSLVPVVICFLFGAYVLRMNRALLFGA
                     MMGARTCAPAMEIISDTARSNIPALGYAGTYAIANVLLTLAGTIIVMVWPGLG"
     gene            8401..8778
                     /locus_tag="OO357_001723"
     CDS             8401..8778
                     /locus_tag="OO357_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YbjM"
                     /protein_id="HCR5886696.1"
                     /translation="MKHKQRWAGAICCFVLFIVVCLFLATHMKGAFRAAGHPEIGLLF
                     FILPGAVASFFSQRREVLKPLFGAMLAAPCSMLIMRLFFSPTRSFWQELAWLLSAVFW
                     CALGALCFLFISSLFKPQHRKNQ"
     gene            complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="OO357_001724"
     CDS             complement(8808..9065)
                     /gene="grxA"
                     /locus_tag="OO357_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 1"
                     /protein_id="HCR5886697.1"
                     /translation="MQTVIFGRSGCPYCVRAKDLAEKLSNERDDFQYQYVDIRAEGIT
                     KEDLQQKAGKPVETVPQIFVDQQHIGGYTDFAAWVKENLDA"
     gene            9225..9512
                     /locus_tag="OO357_001725"
     CDS             9225..9512
                     /locus_tag="OO357_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1418 family protein"
                     /protein_id="HCR5886698.1"
                     /translation="MRAIGKLPKGVLILEFIGMMLLAVALLSVSDSLSLPEPFSRPEV
                     QILMIFLGVLLMLPAAVVVILQVAKRLAPQLMNCPPQYSRSEREKDNDANH"
     gene            9496..10218
                     /gene="nfsA"
                     /locus_tag="OO357_001726"
     CDS             9496..10218
                     /gene="nfsA"
                     /locus_tag="OO357_001726"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase NfsA"
                     /protein_id="HCR5886699.1"
                     /translation="MTPTIELICGHRAIRHFTDEPISEAQCEAIINSARATSSSSFLQ
                     CSSIIRITDKALREELVTLTGGQKHVAQAAEFWVFCADFNRHLQICPDAQLGLAEQLL
                     LGVVDTAMMAQNALTAAESLGLGGVYIGGLRNNIEAVTKLLKLPQHVLPLFGLCLGWP
                     ADNPDLKPRLPASILVHENSYQPLDKDALAQYDEQLAEYYLTRGSNNRWDTWSDHIRR
                     TIIKESRPFILDYLHKQGWATR"
     gene            10279..11181
                     /gene="rimK"
                     /locus_tag="OO357_001727"
     CDS             10279..11181
                     /gene="rimK"
                     /locus_tag="OO357_001727"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HCR5886700.1"
                     /translation="MKIAILSRDGTLYSCKRLREAAIQRGHLVEILDPLSCYMNINPA
                     ASSIYYKGRKLPHFDAVIPRIGTAITFYGTAALRQFEMLGSYPLNESVAIARARDKLR
                     SMQLLARQGIDLPVTGIAHSPDDTSDLIDMVGGAPLVVKLVEGTQGIGVVLAETRQAA
                     ESVIDAFRGLNAHILVQEYIKEAQGCDIRCLVVGDEVVAAIERRAKEGYFRSNLHRGG
                     AASVASITPQEREIAIKAARTMALDVAGVDILRANRGPLVMEVNASPGLEGIEKTTGI
                     DIAGKMIRWIERHATTEYCLKTGG"
     gene            11269..11745
                     /locus_tag="OO357_001728"
     CDS             11269..11745
                     /locus_tag="OO357_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HCR5886701.1"
                     /translation="MTSLVVPGLDTLRQWLDDLGMSFFECDNCQALHLPHMQNFDGVF
                     DAKIDLIDNTILFSAMAEVRPSAVLPLAADLSAINASSLTVKAFLDMQDDNLPKLVVC
                     QSLSVMQGVTYEQFAWFVRQSEEQISMVILEANAHQLLLPTDDEGQNNVTENYFLH"
     gene            12097..13209
                     /gene="potF"
                     /locus_tag="OO357_001729"
     CDS             12097..13209
                     /gene="potF"
                     /locus_tag="OO357_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine/putrescine ABC transporter
                     substrate-binding protein PotF"
                     /protein_id="HCR5886702.1"
                     /translation="MTALNKKWLSGLVAGALMAVSVGTLAAEQKTLHIYNWSDYIAPD
                     TVANFEKETGIKVVYDVFDSNEVLEGKLMAGSTGFDLVVPSASFLERQLTAGVFQPLD
                     KSKLPEWKNLDPELLKLVAKHDPDNKFAMPYMWATTGIGYNVDKVKAVLGENAPVDSW
                     DLILKPENLEKLKSCGVSFLDAPEEVFATVLNYLGKDPNSTKADDYTGPATDLLLKLR
                     PNIRYFHSSQYINDLANGDICVAIGWAGDVWQASNRAKEAKNGVNVSFSIPKEGAMAF
                     FDVFAMPADAKNKDEAYQFLNYLLRPDVVAHISDHVFYVNANKAATPLVSAEVHENPG
                     IYPPADVRAKLFTLKVQDPKIDRVRTRAWTKVKSGK"
     gene            13304..14437
                     /gene="potG"
                     /locus_tag="OO357_001730"
     CDS             13304..14437
                     /gene="potG"
                     /locus_tag="OO357_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000996010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter ATP-binding subunit
                     PotG"
                     /protein_id="HCR5886703.1"
                     /translation="MNDAIPRPQAKTRKALTPLLEIRNLTKSYDGQHAVDDVSLTIYK
                     GEIFALLGASGCGKSTLLRMLAGFEQPSAGQIMLDGVDLSQVPPYLRPINMMFQSYAL
                     FPHMTVEQNIAFGLKQDKLPKAEISSRVNEMLGLVHMQEFAKRKPHQLSGGQRQRVAL
                     ARSLAKRPKLLLLDEPMGALDKKLRDRMQLEVVDILERVGVTCVMVTHDQEEAMTMAG
                     RIAIMNRGKFVQIGEPEEIYEHPTTRYSAEFIGSVNVFEGVLKERQEDGLVLDSPGLV
                     HPLKVDADASVVDNVPVHVALRPEKIMLCEEPPANGCNFAVGEVIHIAYLGDLSVYHV
                     RLKSGQMISAQLQNAHRHRKGLPTWGDEVRLCWEVDSCVVLTV"
     gene            14447..15400
                     /gene="potH"
                     /locus_tag="OO357_001731"
     CDS             14447..15400
                     /gene="potH"
                     /locus_tag="OO357_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotH"
                     /protein_id="HCR5886704.1"
                     /translation="MNTLEPAAQSKPPGGFKLWLSQLQMKHGRKLVIALPYIWLILLF
                     LLPFLIVFKISLAEMARAIPPYTELMEWADGQLSITLNLGNFLQLTDDPLYFDAYLQS
                     LQVAAISTFCCLLIGYPLAWAVAHSKPSTRNILLLLVILPSWTSFLIRVYAWMGILKN
                     NGVLNNFLLWLGVIDQPLTILHTNLAVYIGIVYAYVPFMVLPIYTALIRIDYSLVEAA
                     LDLGARPLKTFFTVIVPLTKGGIIAGSMLVFIPAVGEFVIPELLGGPDSIMIGRVLWQ
                     EFFNNRDWPVASAVAIIMLLLLIVPIMWFHKHQQKSVGEHG"
     gene            15397..16242
                     /gene="potI"
                     /locus_tag="OO357_001732"
     CDS             15397..16242
                     /gene="potI"
                     /locus_tag="OO357_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706737.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine ABC transporter permease PotI"
                     /protein_id="HCR5886705.1"
                     /translation="MNNLPVVRSPWRIVILLLGFTFLYAPMLMLVIYSFNSSKLVTVW
                     GGWSTRWYGELLRDDAMMSAVGSSLTIAACAATAAAILGTIAAVVLVRFGRFRGSNGF
                     AFMITAPLVMPDVITGLSLLLLFVALAHAIGWPADRGMLTIWLAHVTFCTAYVAVVIS
                     SRLRELDRSIEEAAMDLGATPLKVFFVITLPMIMPAIISGWLLAFTLSLDDLVIASFV
                     SGPGATTLPMLVFSSVRMGVNPEINALATLILGAVGIVGFIAWYLMARAEKQRIRDIQ
                     RARRG"
     gene            16302..16790
                     /locus_tag="OO357_001733"
     CDS             16302..16790
                     /locus_tag="OO357_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjO family protein"
                     /protein_id="HCR5886706.1"
                     /translation="MEDETLGFFKKTSSSHARLNVPALVQVAALAIIMIRGLDVLMIF
                     NTLGVRGIGEFIHRSVQTWSLTLVFLSSLVLVFIEIWCAFSLVKGRRWARWLYLLTQI
                     TAASYLWAASLGYGYPELFSIPGESKREIFHSLMLQKLPDMLILMLLFVPSTSRRFFQ
                     LQ"
     gene            16831..17958
                     /gene="rlmC"
                     /locus_tag="OO357_001734"
     CDS             16831..17958
                     /gene="rlmC"
                     /locus_tag="OO357_001734"
                     /EC_number="2.1.1.189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(747)-C(5))-methyltransferase
                     RlmC"
                     /protein_id="HCR5886707.1"
                     /translation="MQCALYDAGRCRSCQWITQPIPEQLSAKTADLKNLLADFPVEEW
                     CAPVSGPEQGFRNKAKMVVSGSVEKTLLGMLHRDGTPEDLCDCPLYPASFAPVFAALK
                     PFIARAGLTPYNVARKRGELKYILLTESQSDGGMMLRFVLRSDTKLAQLRKALPWLHE
                     QLPQLKVITVNIQPVHMAIMEGETEIYLTEQQALAERFNDVPLWIRPQSFFQTNPAVA
                     SQLYATARDWVRQLPVKHMWDLFCGVGGFGLHCATPDMQLTGIEIAPEAIACAKQSAA
                     ELGLTRLQFQALDSTQFATAQGEVPELVLVNPPRRGIGKPLCDYLSTMAPRFIIYSSC
                     NAQTMAKDIRELPGYRIERVQLFDMFPHTAHYEVLTLLVKQ"
     gene            complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="OO357_001735"
     CDS             complement(18133..18864)
                     /gene="artJ"
                     /locus_tag="OO357_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein ArtJ"
                     /protein_id="HCR5886708.1"
                     /translation="MKKLVLAALLASFTFGASAAEKINFGVSATYPPFESIGANNEIV
                     GFDIDLAKALCKQMQAECTFTNHAFDSLIPSLKFRKYDAVISGMDITPERSKQVSFTT
                     PYYENSAVVISKKDTYKTFADLKGKRIGMENGTTHQKYIQDQHPEVKTVSYDSYQNAF
                     IDLKNGRIDGVFGDTAVVNEWLKTNPQLGVATEKVTDPQYFGTGLGIAVRPDNKALLE
                     KLNNALAAIKADGTYQKINDQWFPQ"
     gene            complement(19156..19824)
                     /gene="artM"
                     /locus_tag="OO357_001736"
     CDS             complement(19156..19824)
                     /gene="artM"
                     /locus_tag="OO357_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtM"
                     /protein_id="HCR5886709.1"
                     /translation="MFEYLPELMKGLHTSLTLTVASLIVALILALIFTIILTLKTPVL
                     VWLVRGYITLFTGTPLLVQIFLIYYGPGQFPTLQEYPALWHLLSEPWLCALIALSLNS
                     AAYTTQLFYGAIRAIPEGQWQSCSALGMSKKDTLAILLPYAFKRSLSSYSNEVVLVFK
                     STSLAYTITLMEVMGYSQLLYGRTYDVMVFGAAGIIYLVVNGLLTLMMRLIERKALAF
                     ERRN"
     gene            complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="OO357_001737"
     CDS             complement(19824..20540)
                     /gene="artQ"
                     /locus_tag="OO357_001737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter permease ArtQ"
                     /protein_id="HCR5886710.1"
                     /translation="MNEFFPLASAAGMTVGLAVCALIVGLALAMFFAVWESAKWRPVA
                     WAGSALVTILRGLPEILVVLFIYFGSSQLLLTLSDGFTINLGFVQIPVQMDIENFDVS
                     PFLCGVIALSLLYAAYASQTLRGALKAVPVGQWESGQALGLSKSAIFFRLVMPQMWRH
                     ALPGLGNQWLVLLKDTALVSLISVNDLMLQTKSIATRTQEPFTWYIVAAAIYLVITLL
                     SQYILKRIDLRATRFERRPS"
     gene            complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="OO357_001738"
     CDS             complement(20547..21278)
                     /gene="artJ"
                     /locus_tag="OO357_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5886711.1"
                     /translation="MKKVLIAALIAGFSLSATAAETIRFATEAPYPPFESIDANNQIV
                     GFDVDLAQALCKEIDATCTFSNQAFDSLIPSLKFRRVEAVMAGMDITPEREKQVLFTT
                     PYYDNSALFVGQQGKYTSVDQLKGKKVGVQNGTTHQKFIMDKHPEITTVPYDSYQNAK
                     LDLQNGRIDSVFGDTAVVTEWLKDNPKLAAVGDKVTDKDYFGTGLGIAVRQGNTELQQ
                     KLNTALEKVKKDGTYETIYNKWFQK"
     gene            complement(21296..22024)
                     /gene="artP"
                     /locus_tag="OO357_001739"
     CDS             complement(21296..22024)
                     /gene="artP"
                     /locus_tag="OO357_001739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine ABC transporter ATP-binding protein
                     ArtP"
                     /protein_id="HCR5886712.1"
                     /translation="MSIQLNGINCFYGAHQALFDITLDCPQGETLVLLGPSGAGKSSL
                     LRVLNLLEMPRSGTLNIAGNHFDFTKTPSDKAIRDLRRNVGMVFQQYNLWPHLTVQQN
                     LIEAPCRVLGLSKDQALARAEKLLERLRLKPYSDRYPLHLSGGQQQRVAIARALMMEP
                     QVLLFDEPTAALDPEITAQIVSIIRELAETNITQVIVTHEVEVARKTASRVVYMENGH
                     IVEQGDASCFTEPQTEAFKNYLSH"
     gene            complement(22242..22757)
                     /locus_tag="OO357_001740"
     CDS             complement(22242..22757)
                     /locus_tag="OO357_001740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5886713.1"
                     /translation="MRYRSLSLLIPCALLLSACTTVTPAYKDNGPRTGSCVQGGPDSV
                     AQQFYDYRIQHRSNDITALRPYLSDKLATQLSDASRDNSHRELLSSDPFSSRTTLPDS
                     AHVASASTIPNRDARNIPLRVDLKQGDQGWQDEVLMIQEGQCWVIDDVRYLGGSVHAT
                     AGTLRQSIENR"
     gene            23406..25175
                     /locus_tag="OO357_001741"
     CDS             23406..25175
                     /locus_tag="OO357_001741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5886714.1"
                     /translation="MINNISDQASSFPGTQLNQSDNFLDSLREFFAILNPSRKGELST
                     WDTIYLHLILAINADSDLIKNDVLLAENIPSANYQFNTFFSNTFEIDVKKYLGKSEDN
                     EVEIKAGNERISIGIRNVSNGKLERQQFLFPLDYENKLQEQLDKYFTIESHPLLYRYT
                     IGSKIANVIFEKLYSRIDFNKEQYISFIKDAFIHFYDYSRRYAISENIDKDAVTNNIA
                     LMSTFYDSDNTSGEVLNNDFTEEESFETALDVEHAIVLGFADDNFETKPVHYQDLLTR
                     FSAFQDTVFNLFPEMHSSHYHDICSVSVDMTKGTQCMIHLMVNEEVFMSLPVPVATMV
                     REDASNLVNLKTLLNDGCFIKYSHFNDVALIKQNISNLYLSHTVVNESILKKCCFENG
                     SLGDVKITNSNVINSAFKNISFRSVKINNVNTHSLKFINCNFFNVDMIRVNLSKCLFH
                     ECSMHGVKIKPWLPVKWTKELISDYLYGCLLSLYSICARDIYNMNAGNNVKVAADAFL
                     EIIFSLKNKYCIKLLSAQDRAFIYEFARMIFAYINDKSIEILLLSCFAAADQKAIQRY
                     RPQSQDGEDFRSHLQYKLPLSAH"
     gene            25386..25709
                     /locus_tag="OO357_001742"
     CDS             25386..25709
                     /locus_tag="OO357_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HCR5886715.1"
                     /translation="MQFSTTPTLEGLTIVEYCGVVTGEAILGANIFRDFFAGIRDIVG
                     GRSGAYEKELRKAREIAFEELGSQARALGADAVVGIDIDYETVGQNGSMLMVSVSGTA
                     VKTRR"
     gene            25706..26536
                     /gene="amiD"
                     /locus_tag="OO357_001743"
     CDS             25706..26536
                     /gene="amiD"
                     /locus_tag="OO357_001743"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiD"
                     /protein_id="HCR5886716.1"
                     /translation="MRRVFWLVAAALLLAGCAGEKGIVEKEGYQLDTRHQAQAAYPRI
                     KVLVIHYTADDFDSSLATLTDKQVSSHYLIPAVPPRYNGKPRIWQLVPEQELAWHAGI
                     SAWRGATRLNDTSIGIELENRGWQKSSGVKYFAPFEQAQIQALIPLAKDIIARYHIKP
                     ENVVAHADIAPQRKDDPGPLFPWQQLAQQGIGAWPDAQRVNFYLAGRAPHTPVDTASL
                     LELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIAEALLEKYGQD
                     "
     gene            complement(26533..27546)
                     /locus_tag="OO357_001744"
     CDS             complement(26533..27546)
                     /locus_tag="OO357_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415389.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HCR5886717.1"
                     /translation="MKVLVTGATSGLGRNAVEFLCQKGISVRASGRNEAMGKLLEKMG
                     AEFVPTDLTELVSSQAKVMLAGIDTLWHCSSFTSPWGTQQAFDLANVRATRRLGEWAV
                     AWGVRNFIHISSPSLYFDYHHHRNIKEDFRPHRFANEFARSKAASEEVINMLSQANPQ
                     TRFTILRPQSLFGPHDKVFIPRLAHMMHHYGSILLPHGGSALVDMTYYENAVHAMWLA
                     SQEACDKLPSGRVYNITNGEHRTLRSIVQKLIDELNIDCRIRSVPYPMLDMIARSMER
                     LGRKSAKEPPLTHYGASKLNFDFTLDITRAQEELGYQPVITLDEGIEKTAAWLRDHGK
                     LPR"
     gene            complement(27645..29075)
                     /locus_tag="OO357_001745"
     CDS             complement(27645..29075)
                     /locus_tag="OO357_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2867 domain-containing protein"
                     /protein_id="HCR5886718.1"
                     /translation="MPQRILVLGASGYIGQHLVRTLSQQGHQILAAARHVDRLAKLQL
                     ANVSCHKVDLSWPDNLPALLQDIDTVYFLVHSMGEGGDFIAQERQLALNVRDALREVP
                     VKQLIFLSSLQAPPHEQSDHLRARQATADILREANVPVTELRAGIIVGAGSAAFEVMR
                     DMVYNLPVLTPPRWVRSRTTPIALENLLHYLVALLDHPANEHRIFEAAGPEVLSYQQQ
                     FEHFMAVSGKRRWLIPIPLPTRWISVWFLNVITSVPPTTARALIQGLKHDLLADDTAL
                     RALIPQRLIAFDDAVRSTLKEEEKLVNSSDWGYDAQAFARWRPEYGYFAKQAGFTVKT
                     SASLAALWLVVNQIGGKERYFFGNILWQTRALMDRAIGHNLAKGRPEREYLQTGDAVD
                     SWKVIVVEPKKQLTLLFGMKAPGLGRLCFTLEDKGDYRTIDVRAFWHPHGMPGLFYWL
                     LMIPAHLFIFRGMAKQIARLAEQSTD"
     gene            complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="OO357_001746"
     CDS             complement(29086..30087)
                     /gene="ltaE"
                     /locus_tag="OO357_001746"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HCR5886719.1"
                     /translation="MIDLRSDTVTRPSRAMLEAMMAAPVGDDVYGDDPTVNALQDYAA
                     ELSGKEAAIFLPTGTQANLVALLSHCERGEEYIVGQAAHNYLFEAGGAAVLGSIQPQP
                     IDAAADGTLPLDKVAMKIKPDDIHFARTKLLSLENTHNGKVLPREYLKEAWEFTRERN
                     LALHVDGARIFNAVVAYGCELKEITQYCDSFTICLSKGLGTPVGSLLVGNRDYIKRAI
                     RWRKMAGGGMRQSGILAAAGMYALENNVARLQEDHDNAAWMAEQLREAGADVMRQDTN
                     MLFVRVGEENAAALGEYMKARNVLINASPIVRLVTHLDVSREQLAEVAAHWRAFLAR"
     gene            complement(30124..31872)
                     /gene="poxB"
                     /locus_tag="OO357_001747"
     CDS             complement(30124..31872)
                     /gene="poxB"
                     /locus_tag="OO357_001747"
                     /EC_number="1.2.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone-dependent pyruvate dehydrogenase"
                     /protein_id="HCR5886720.1"
                     /translation="MKQTVAAYIAKTLESAGVKRIWGVTGDSLNGLSDSLNRMGTIEW
                     MSTRHEEVAAFAAGAEAQLSGELAVCAGSCGPGNLHLINGLFDCHRNNVPVLAIAAHI
                     PSSEIGSGYFQETHPQELFRECSHYCELVSSPEQIPQVLAIAMRKAVLNRGVSVVVLP
                     GDVALKPAPEGATTHWYHAPQPVVMPEEEELRKLAQLLRYSSNIALMCGSGCAGAHKE
                     LVEFAGKIKAPIVHALRGKEHVEYDNPYDVGMTGLIGFSSGFHTMMNADTLVLLGTQF
                     PYRAFYPTDAKIIQIDINPASIGAHSKVDMALVGDIKSTLRALLPLVEEKTDRKFLDK
                     ALEDYRDARKGLEDYRDARKGLDDLAKPSEKAIHPQYLAQQISHFAADDAIFTCDVGT
                     PTVWAARYLKMNGKRRLLGSFNHGSMANAMPQALGAQATEPERQVVAMCGDGGFSMLM
                     GDFLSVVQMKLPVKIIVFNNSVLGFVAMEMKAGGYLTDGTELHDTNFARIAEACGITG
                     IRVEKASEVDEALQRAFSIDGPVLVDVVVAKEELAIPPQIKLEQAKGFSLYMLRAIIS
                     GRGDEVIELAKTNWLR"
     gene            complement(32005..32973)
                     /gene="hcr"
                     /locus_tag="OO357_001748"
     CDS             complement(32005..32973)
                     /gene="hcr"
                     /locus_tag="OO357_001748"
                     /EC_number="1.6.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidoreductase"
                     /protein_id="HCR5886721.1"
                     /translation="MTMPTNQCPWRIQVHHITQETPDVWTISLICHDHYPYRAGQYAL
                     VSVRNSAETLRAYTISSTPGVSEYITLTVRRIDDGVGSQWLTRDVKRGDYLWLSDAMG
                     EFTCDDKAEDKFLLLAAGCGVTPIMSMRRWLAKNRPQADVRVIYNVRTPQDVIFADEW
                     RNYPVTLVAENNVTEGFIAGRLTRELLADVPDLASRTVMTCGPAPYMDWVEQEVKALG
                     VTRFFKEKFFTPVAEAATSGLKFTKLQPAREFYAPVGTTLLEALESNNVPVVAACRAG
                     VCGCCKTKVVSGEYTVSSTMTLTDAEIAEGYVLACSCHPQGDLVLA"
     gene            complement(32985..34637)
                     /gene="hcp"
                     /locus_tag="OO357_001749"
                     /gene_synonym="priS"
     CDS             complement(32985..34637)
                     /gene="hcp"
                     /locus_tag="OO357_001749"
                     /gene_synonym="priS"
                     /EC_number="1.7.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxylamine reductase"
                     /protein_id="HCR5886722.1"
                     /translation="MFCVQCEQTIRTPAENGCSYAQGMCGKTAETSDLQDLLIAALQG
                     LSAWAVKAREYGIINHDVDSFAPRAFFSTLTNVNFDSPRIVGYAREAIALREALKAQC
                     LAVDANARVDNPMADLQLVSDDLGELQRQAAEFTPNKDKAAIGENILGLRLLCLYGLK
                     GAAAYMEHAHVLGQYDNDIYAQYHKIMAWLGTWPADMNALLECSMEIGQMNFKVMSIL
                     DAGETGKYGHPTPTQVNVKATAGKCILISGHDLKDLYNLLEQTEGTGVNVYTHGEMLP
                     AHGYPELRKFKHLVGNYGSGWQNQQVEFARFPGPIVMTSNCIIDPTVGAYDDRIWTRS
                     IVGWPGVRHLDGEDFSAVIAQAQQMAGFPYSEIPHLITVGFGRQTLLGAADTLIDLVS
                     REKLRHIFLLGGCDGARGERHYFTDFATSVPDDCLILTLACGKYRFNKLEFGDIEGLP
                     RLVDAGQCNDAYSAIILAVTLAEKLGCGVNDLPLSLVLSWFEQKAIVILLTLLSLGVK
                     NIVTGPTAPGFLTPDLLAVLNEKFGLRSITTVEEDMKQLLNA"
     gene            complement(34781..35680)
                     /gene="lysO"
                     /locus_tag="OO357_001750"
     CDS             complement(34781..35680)
                     /gene="lysO"
                     /locus_tag="OO357_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lysine exporter LysO"
                     /protein_id="HCR5886723.1"
                     /translation="MFSGLLIILVPLIVGYLIPLRQQAALKAINQLLSWMVYLILFFM
                     GISLAFLDNLASNLLAILHYSAVSITVILLCNIAALMWLERGLPWRNHHQQEKLPSRI
                     AMALESLKLCGVVVIGFAIGLSGLAFLQHATEASEYTLILLLFLVGIQLRNNGMTLKQ
                     IVLNRRGMIVAVVVVVSSLIGGLINAFILDLPINTALAMASGFGWYSLSGILLTESFG
                     PVIGSAAFFNDLARELIAIMLIPGLIRRSRSTALGLCGATSMDFTLPVLQRTGGLDMV
                     PAAIVHGFILSLLVPILIAFFSA"
BASE COUNT         8907 a         8847 c         9189 g         8818 t
ORIGIN      
        1 cgtgaccata ccgcgtgggc agctgaaaaa cctgaaggcg agctacacgt taacggaacc
       61 gcagcttacc gcaccgctga aaaaaggtca ggttgtcggg accattgatt tccagcttaa
      121 cggtaaatcc attgagcagc gtccgctgat cgtgatggaa aatgtggaag agggcggatt
      181 ctttggtagg atgtgggatt tcgtgatgat gaaattccat cagtggttcg gcagctggtt
      241 ctcttaatct tctgataacc ggatggcgcg aaacgtcatc cggttatacg tcattaatac
      301 atcaacttaa tacggtgcgt ctgcgcgtac ttcacatact catcttccgg gcaacaatca
      361 ctcaccacaa tatcaaagcg tttcaggtca cccatgcgcg ccggacgcac cttgccaaat
      421 ttactgtggt cgacaaccag cacatgcttt tgcgccatcg acatggccca gtgttttacc
      481 ggcaactctt caagattaaa acaggtagcg cctttactga catgcacgcc cgccgcggaa
      541 taaaaagcga tatccgggca aaaattattc aacgtttgct gaaaatcgat gggtttgaaa
      601 atggcgttgc tggcgtgaaa ttcaccaccg cacagaaatg cgcggcaatg gggtttctct
      661 ttcagcgcca gaaaggtatt tagcgaataa caaacggcgg taaaagggat ttcattatca
      721 atcgcttcaa taatccacgg cgtggtggtg ccacagtcaa aaaagagggt ctgatcgggt
      781 tctaccagtg tcgcagccag ttttgccgcc cggcgttttt cttccaccag gcgggatttt
      841 tgatcgctta acaggtaatg gctggcactg cgcggttcca gaacaatata gccgccgagc
      901 aaaacgacgg gcgcactgtg gttgttcaga tcgcgacgaa tcgtcatctc cgaaacccca
      961 agcagggcgg cggcgtcttt aagatgtaac ttatcgctgc gttttaattc ttgcagcagc
     1021 tgcccgatac gctcttcgcg acgtgtttcc ataatccctc tgaatagtta ttgaagcgag
     1081 ccgctcaata ctacactttt tagcagagat cagtcacgca cccagccttt gcggatcggt
     1141 aatgcaaaac agacgcgata ccacgattgc agacgttgat agagtttatg ccccatcgct
     1201 tgccagataa tctgggcact aaggcagcca atcatacctg ccagcaatcc accgagcata
     1261 tccagcggcc agtggacgcc aagataaacg cgcgaccagg caatgacgac ggccagcacc
     1321 attaaaagtg agccggacca caggcgatgc cagcataaaa atgccagtgc aaaggtgaaa
     1381 atcaccgtac cgtgatcgct cgggaatgag tcatccgccg catgatgcag gaagttatag
     1441 ccgatatttt cgacaaaggg tcggtcgtgc ggaaaaagat gtcccatcgt ccaggacaca
     1501 aacaggctga ccgccagcgc gatagcgatt tttatcacca gttgccgttg tgctgtaagc
     1561 ccccacaacc aaagtaccgc ggccagcaac ggcaccacgg taatcaaatc tttagcaata
     1621 aaaatcgcca acgagatcat ccacggagcc gagtctggcg tcgcgttaat aagagagaat
     1681 agagagagat tcaaattttc cagcataact tcccgacgca aagtgattaa aagggagcca
     1741 atacaggcaa gtcgttgaga ataaagtgca ggttaaactg ggtaaagcgg catcgtctta
     1801 tttccctcaa gcggcctgtt tacggtgggt gattgtaacg ggcataggtt aaataaaact
     1861 taaagaaagc gtagctatac tcgcaataat gtaagaatgt gcttaaccgt ggtttcagct
     1921 acaaaattcg ctttctcgtt agctgcgctt ttattaaact ctgcgcgatt attattggcg
     1981 aagaaattgc atgcaaaata aattagcttc cggtgccagg cttggacgtc aggcgttact
     2041 tttccctctc tgtctggtgc tttacgaatt ttcaacctat atcggcaacg atatgatcca
     2101 acccggtatg ttggccgtgg tggaacaata tcaggcgggc attgattggg ttcctacttc
     2161 gatgaccgcg tatctggcgg gcgggatgtt tttacaatgg ctcctggggc cgctgtcgga
     2221 tcgtattggt cgccgtccgg tgatgctggc gggtgtggtg tggtttatcg tcacctgtct
     2281 ggcaatatta ctggcgcaaa acattgaaca attcaccctg ttgcgcttct tgcagggcat
     2341 aagcttctgt ttcattggcg ctgtgggata cgccgcaatt caggaatcct tcgaagaggc
     2401 ggtttgtatc aagatcaccg cgctgatggc gaacgtggcg ctgattgctc cgctacttgg
     2461 tccgctggtg ggcgcggcgt ggatccatgt gctgccttgg gaggggatgt ttgttttgtt
     2521 tgccgcattg gcagcgatct cctttttcgg tctgcaacga gctatgcctg aaaccgccat
     2581 gcgtataggc gagaaactgt cactgaaaga actcggtcgt gactataagc tggtgctgaa
     2641 gaacggccgc tttgtggcgg gggcgctggg attcgttagc ctgccattgc tggcgtggat
     2701 cgcccagtcg ccgattatca tcattaccgg ggagcagttg agcagctatg aatatggctt
     2761 gctgcaagtg cctattttcg gggcgttaat tgcgggtaac ttgctgttag cgcgtctgac
     2821 ctcgcgccgc accgtacgtt cgctgattat tatgggcggc tggccgatta tgattggtct
     2881 gttggtcgct gctgcggcaa cggttatctc atcgcacgcg tatttatgga tgaccgccgg
     2941 gttaagtatt tatgctttcg gtattggtct ggcgaatgcg ggactggtgc gattaaccct
     3001 gtttgccagc gatatgagta aaggtacggt ttctgcggcg atgggaatgc tgcaaatgct
     3061 gatctttacc gtcggtattg aaatcagcaa acatgcctgg ctgaacgggg gcaacggact
     3121 gtttaatctc ttcaaccttg tcaacggaat tttgtggctg tcgctgatgg ttatcttttt
     3181 aaaagataaa cagatgggaa attctcacga agggtaaaaa aatgcctgat tgatttgtgc
     3241 gatcaggcat tctcgaatta atggtgatgg tcgtcaatct ggtgttcgat aaccatccct
     3301 tcacctacgc tggcaagatg gcgaacataa ggatgcgggc ggtaagccgg agctggcgca
     3361 ggagccacat agacggtttg cggcacagtc gcgacgctga ccgcttgtgg aacgctgact
     3421 gaatcaggaa ccacaaccac tttgtatcca ctcggcacat cgacggtgac actttgcgcc
     3481 atcgccacac cagtaaagcc cattaaaagc gcgcccaaca gtagacaatt tttcataatc
     3541 atttcgcctg aaataatgca gcgggttaat ttccgcgaat tatgcagcct tcattcaggc
     3601 attgtgttgc cgggcgtgct gccgttttta ttggtcaaat ggcgcttcgt gtttaagaac
     3661 tttatcgatc acatccagta cgccttcacg gttattgtag cctgcccggt attttgccgc
     3721 tgcgacgact gcgctgccgg cattttccat tgcaaaacta aagcctgcct gacgcagcat
     3781 ctcaatatcg ttaccgccat cgccaaagac caccacttcg ctgtcgtcta ttccccataa
     3841 tttctgcagt tggcgaaggc cattggcttt atgtacgccg gggataatca gatcgatgct
     3901 gccgttgccg gtgtggactg gcaccataat atcgccgatg gcctcatgta atgctttttg
     3961 tacttgtgga atcagttcat cggaaagatt caggccaaac ttaaagaaaa tatcttctaa
     4021 gttgtcaaag ttatcgacgt attccagacg gtgataatac atttccgcca ccgttttcat
     4081 ggcatcgtca tattttttga gtgtataggc gctatttttt ccgcaggcaa taatttccac
     4141 ttccgggcgc gtcagcaaat gttccacgac agtagcaaac gcatccttcg acagctcgcc
     4201 attaaaaaca tctttgcctt cactcattac ccagccgccg ttttcagcca caaaggcaat
     4261 ttcattcgca atttcaggga agaaagagat caactgataa tattgattcc cgctggcgac
     4321 cacaaagcga atgccttgtg ctttcatttg ctgatactga gccataaacc gctcacggtt
     4381 ataggttttt tgatcgctta agaaagtacc atccatgtct accgcaatta atttaatgct
     4441 catcaactat tctccatcgc cgtctgcgtt ttggtatcgg gtttggcgac ggctttcgcg
     4501 acaatagccg cgagaataac cagcgccagt acaaccagca ttgcactacg taatccataa
     4561 tgttcgccga gatagcccag cagcggcggc ccgacgagga aagccagata accggtcgtt
     4621 gctaccacac tgacgcgggt tggtgcatcg gggccggtat cgctggcggc agaaatggtc
     4681 agcgggaagc ccagcgaggc acccagtccc caaagtacaa cagacactcc tgcgacccag
     4741 gcgctatcga caaaaataat cagcccaata cccaacgccc ccattagtgc actggcccga
     4801 accacggcaa cgcgactgta actgtcgatg aaccaaccgc cggtgaagcg tccaacggtc
     4861 atccccaggg taaaaccggc ataaatcagt gagccggaag tggggctaaa accgtgacca
     4921 tcaaccatta ataagggtaa ccagtcgttg gcagaacctt cggcaaaggc catcgccagc
     4981 actacaacac caatcaacag caactggata tcgcgataaa aaggaatgcc tttttcgcca
     5041 tgctgggtgc catcggcagc atttttgccc gtaccgtcag ggattgcctg aatggcgata
     5101 taaataggcg cgatacctac cagcgccgcc aataaaatgt gcaccgttgc cggaacgcca
     5161 aaggccgtca gtgccatccc gacaccagcg cctgccagcg tgcccaggct ataaaaaccg
     5221 tgcatcatcg gcaaaaccgt tttattcatt tctcgctcaa cggcggcacc ttcaacgttt
     5281 atcgccactt ccgcagaacc aaaacttgcc ccaaagacgc cgagaccaac ggcaaagagc
     5341 aggggcgatg tcagccagag tgccagactt aatatcatca tcccgatcaa tgcgcaggac
     5401 atcgtgacca ggatgacatt acgtgtccca aagcgtttca ctaaccacgc cgagcagaga
     5461 ataccgctca tcgaaccgat cgacagacca aagagaacac cgcccatttc agcgatcgag
     5521 acagagagaa tatcgcggat agcaggcgta cgggttgccc aggacgccat taacaggcct
     5581 ggcaaaaaga agaacataaa cagcgcccag gttcggcgtt tcaatgcatt acgtgaagaa
     5641 tttacggtca tagatcacgt caaaataaga agagggaaga caacattagc aaggttgtgt
     5701 acatttgtac acaattgcag acagaggaaa tgacatgcgt cgcgctaacg atccgcaacg
     5761 gcgagaaaaa attatccagg ccacgctgga ggcggtgaaa ctttacggaa tacatgctgt
     5821 tacgcaccgc aaaattgcta cccttgccgg ggtaccgttg ggatcgatga cctactattt
     5881 ttcaggaatt gatgagttgt tgctggaggc gttcagcagt tttactgaga ttatgtcccg
     5941 gcaatatcag gcatttttta gcgatgttag tgatgctcaa ggcgcatgcc aggctatcac
     6001 cgatatgatc tacagctcac aggttgcaac gccggataat atggagctga tgtaccagct
     6061 ctacgcgctg gctagccgaa aaccgctatt aaaaacggta atgcaaaact ggatgcagcg
     6121 cagtcagcaa acgctcgaac aatggtttga acccggaacc gcccgcgcgc ttgatgcgtt
     6181 tattgagggg atgacgctgc attttgtcac cgaccgtaag ccgctatcgc gcgaggagat
     6241 tttgaggatg gttgagaggg ttgcagggta gtagataaat tttagataac aaaaaaccca
     6301 tcaaccttga accgaaatgg cggggttgat gggctccaca aaatggggac atcaaagaaa
     6361 agcagtggca ctaattaaga ctgatgccct gcggaaaagt tctgcggttg tgcaaaaaaa
     6421 tttcattttc agggcaactt cagttttatc ctaatcctgg ccataccatg acgatgattg
     6481 tccctgccag cgtcagcagg acgttggcga ttgcgtaggt gcccgcatag cccagcgcag
     6541 ggatgttact gcgagctgta tcactgatga tctccattgc cggcgcgcag gtgcgtgcgc
     6601 ccatcattgc gccgaacaac agtgcgcggt tcattcgcaa tacataagca ccgaacaaga
     6661 aacagataac cacgggcaca agactgacaa ttaatccggc aatcaacatc tgaccgccaa
     6721 tcgcgcccag gccgttatta ataccgctac cggcgctcag accaacgcct gccataaaca
     6781 ccatcaagcc gaactctttc accatgctta atgcaccttg cggaatgtaa ccgaaggtcg
     6841 ggtggttagc acgcataaag cccagcataa ttccggcgaa taacaacccg gcagcgttcc
     6901 ccatgccgaa actgaatgtg ctgaactgga aggtgatcat cccgatcatc agcccaataa
     6961 caaagaaggc gcaaaatgcc agcaggtcag tgacctggct gtgaatcgag ataaagccga
     7021 tgcgatcggc gatggttttt acgcgacggg catcaccgct gacttgtaaa acgtcacctt
     7081 tgttaagcac gacgttgtca tctatcggca tctcaatctg gctacgaatg acgcggttaa
     7141 ggaagcaacc gtgatcggtc aacttcagtt gtgcgagacg tttacctaca gcgttatggt
     7201 ttttaacgac cacttcttca gtgacgatac gcatgtcgag aaggtcacga tcgaaaactt
     7261 ctttaccgtt acggaagctg ggatcgagtc gggcatgggc gtcgggatag cctaccaacg
     7321 ctatttcatc gcccatttgt agcacggcat caccgtctgg atttgccaga atcccgttac
     7381 gtcgaatacg ttcaatgtag cagccggttt gtcgataaat acccagttca cgcagatttt
     7441 tgccgtcggt ccaggccacc agctccgggc cgacgcgata ggcgcggatc accggtaaat
     7501 aaaccttacg gttggcatca gtgtccaggc cacgttcgcg ggcgatttgc tgggcgctgg
     7561 tctgtaagtc ctgatgctgc aatttcggca agtaacgcgc accaacaatc aaactcacca
     7621 gaccgattaa ataggttaag gcatacccga ggctcagatt atccagtgcc agtgagagct
     7681 gcctgctttc catgccggaa tgatgcagtg tatcgccagc accgaccaga accggtgtcg
     7741 acgtcataga gcctgctaac ataccggccg tcaggccaat atcccagcca aacagcttac
     7801 ctaaccctaa ggcgatcacc agcgcactgc caaccatcac cagtgctaac attaggtaat
     7861 ttttcccatc gcgaaaaaaa atggaaaaaa agttcggtcc ggcttcgacc ccgacgcaga
     7921 aaataaacag cataaagcca agattaagcg catcggtgtt aatgctgaaa tgttgttggc
     7981 ctaataacag cgatacgact aaaacgccaa tggaattacc cagttggatc gaaccaagtc
     8041 gtaactttcc gagacatagc ccaagcgcga ggaccacaaa taataacaga atgtaattcc
     8101 catttaacaa ttcggcgacg tttatattca cggaggctaa cttcttgttt actagtaagc
     8161 tgttgaaaga aatgctaatt tacgataatg tgttttacca gaattcaggg cgcagattca
     8221 ttcagcgcac ctaaacgata gtaaagtaac aatatatttt actagtgtaa tcacattagg
     8281 tatcaacggc tatatgaatt gcgttggcct atattagcat ggaatgcgaa gcggctttat
     8341 cttactgaac gccacactgg cgaaaaatgt gttcgataga cgcagtgtca ggaggaacga
     8401 gtgaaacata aacaacgttg ggcgggggca atctgctgtt ttgtcctctt cattgtggtg
     8461 tgcctttttc tggcgacgca catgaaaggc gcttttcggg ctgccgggca tcctgaaatc
     8521 ggcttgctgt ttttcattct tcctggagca gttgccagtt tcttttcaca gcgtagagaa
     8581 gtcctgaaac ctctatttgg cgcaatgctg gcggcaccct gttcaatgct cattatgcgg
     8641 ctgttttttt caccgacgcg ctcattctgg caagagctgg catggttact aagcgcggtg
     8701 ttctggtgtg cgctgggggc actgtgtttc ttatttatca gtagtttgtt taaaccacag
     8761 cacagaaaaa atcagtaaag ccctcaacgc gagggcttgt cagacgatca ggcgtccaga
     8821 ttttctttca cccatgcagc aaaatcggta tagccgccga tatgttgctg atcgacaaaa
     8881 atctgcggca cggtttctac gggtttacct gccttttgtt gtagatcttc tttagtgatc
     8941 ccttccgcac gaatatctac atactgatac tgaaaatcat cgcgttcatt gctcaatttc
     9001 tcagccagat cttttgcacg cacacagtaa gggcaacccg aacgaccaaa aataacggtt
     9061 tgcattattt ctctcctcat agatttatgc ctgtaatgat cacgctaaaa tgtattcgct
     9121 gaaagtaggt ttaacctgtt gcattaattg ctaaaagcta taactgttaa acacaataca
     9181 gtgaaaagtt ttagactgaa ggctcacttt gcagagggaa gcgtatgcgc gcgatcggta
     9241 aattgcctaa aggcgtgttg atactggaat ttatcggaat gatgctactg gcggtggcgc
     9301 tgctgtcggt aagcgactcc ctgtcgctgc ctgagccatt ttctcggcca gaagtgcaga
     9361 ttctgatgat ttttctcggt gttttgctca tgcttcccgc tgcggtggtg gttattcttc
     9421 aggtggcaaa acgtcttgcc ccacagctga tgaactgtcc accgcaatat tcacgttcag
     9481 aaagagaaaa agataatgac gccaaccatt gaacttattt gtggccatcg cgccattcgc
     9541 catttcactg atgaacccat ttccgaagcg cagtgtgagg cgattattaa cagcgcccgt
     9601 gcgacgtcca gttccagttt tttgcagtgt agtagcatta ttcgcattac cgacaaagca
     9661 ttacgtgaag aactggtgac gctgaccggc gggcaaaaac acgtagcgca agcggcggag
     9721 ttctgggtgt tctgtgccga ctttaaccgc catttacaga tctgtccgga tgctcagctc
     9781 ggcctggcgg aacaactgtt gctcggtgtc gttgatacgg caatgatggc gcagaatgca
     9841 ttaaccgcag cggaatcgct gggattgggc ggggtatata tcggcggcct gcgcaataat
     9901 attgaagcgg tgacgaaact gctgaaatta ccgcagcatg ttctgccgct gtttgggctg
     9961 tgccttggct ggcctgcgga taatccggat ctcaagccgc gtttaccggc ctccattttg
    10021 gtgcatgaaa acagctatca accgctggat aaagacgcac tggcgcagta tgacgagcaa
    10081 ctggcggaat attacctcac ccgtggcagc aataatcgct gggatacctg gagcgatcat
    10141 atccgccgaa caatcattaa agaaagccgc ccatttattc tggattattt gcacaaacag
    10201 ggttgggcga cgcgctaaaa ccgccacgtc gatgtatgat acgcgggctt ttgaccaggt
    10261 ctgacagaga ggtgcagggt gaaaattgcc atattgtccc gggatggaac gctctattcg
    10321 tgtaagcggc tgcgtgaagc cgctatacag cgcggtcacc tggttgaaat tcttgatccg
    10381 ctttcttgct acatgaacat aaatcctgcg gcgtcttcta tttactacaa aggccgcaag
    10441 ttaccccatt ttgacgcagt gatcccgcgt attggcaccg ccattacctt ttatgggacg
    10501 gcggcactgc gccagttcga gatgctgggg agctatccgc tcaatgagtc ggtcgccatt
    10561 gcccgggcgc gtgacaaatt gcgttccatg caactgctgg cgcgtcaggg catcgacctg
    10621 cctgtcacgg gcattgcgca ttcgccggat gataccagcg atttaatcga catggtcggt
    10681 ggtgcgccgc tggtggtcaa gttggttgaa ggcacgcagg gaattggcgt cgtgctggcg
    10741 gagactcgtc aggcggcgga aagcgtgatt gacgctttcc gcggtctgaa cgcgcatatt
    10801 ctggtgcagg aatatatcaa agaggcgcaa gggtgcgata tccgctgtct ggttgttggt
    10861 gatgaagtgg tcgctgcgat tgaacggcgg gcgaaagagg gctattttcg ttccaatttg
    10921 catcgtggcg gcgcggcaag cgtcgccagt atcacaccac aggagcgtga aatcgcgata
    10981 aaagccgcgc gaacgatggc gctggacgtt gctggtgtgg atattctgcg tgctaatcgc
    11041 gggccgttgg tgatggaagt gaatgcgtcg ccggggctgg aaggaataga aaaaaccacc
    11101 ggtatcgata tcgcgggtaa aatgatccgc tggatcgaac gccacgctac gacagaatat
    11161 tgcctgaaaa cgggtggtta gtcgcaatca cattactgat catggttttg cctgcgcttt
    11221 ttgcgtaagc tgtgccggtc tttttatcga aagaggttgt acaaaattat gacatcgctg
    11281 gtcgttcctg gtctggatac gctgcgtcaa tggctcgatg acctggggat gagttttttt
    11341 gaatgtgata actgtcaggc tctgcatctg ccccatatgc agaatttcga cggtgtcttt
    11401 gatgccaaaa tcgatctgat cgataacacg atcctgtttt ctgccatggc ggaagtccga
    11461 ccttcagccg tattgccgct ggcggcggat ttatctgcca tcaatgccag ttcgctgacc
    11521 gtgaaagcat ttcttgatat gcaggatgat aatctgccaa agctggtggt ttgccagtct
    11581 ttatccgtta tgcagggcgt aacctatgag cagtttgcat ggttcgtgcg tcagagcgaa
    11641 gagcagattt cgatggtcat tcttgaagct aatgcccatc aactgctgtt accgactgac
    11701 gatgaagggc aaaacaacgt taccgaaaac tattttctcc actgataatt cctttcgagc
    11761 acgcagtcgc tgccgcagtg actgcgcgct gcaaaattat ctgctgtttt taaccttttc
    11821 ttaaagatta tttcacttct cttgtgtcga tttggcttta tcacatagag caaatacgca
    11881 taaaaatttg ttaaataccg ttttttaatc cgagctatag tctcaaaccc tggctaaagt
    11941 tattcttgcg atgcttctat atagcgagca gtgctggccg ggagagagtt ctcttttctt
    12001 acaccgcgcc gataaaaaaa tatgcacgtt tattgcatat ctttcagtgt gacaactttt
    12061 gttcgtttgt taacgaactt tcagaaggaa agagatgtga ccgccttaaa taaaaaatgg
    12121 ctatcgggtc tggttgcggg tgctctgatg gccgtctctg tcggcacgct cgcggctgaa
    12181 caaaaaacac tccacattta taactggtct gattatatcg cccctgacac ggtggccaat
    12241 tttgaaaaag aaaccggtat taaagtcgtc tacgatgttt tcgactctaa cgaagtactg
    12301 gaaggcaaat taatggcggg tagcaccggc tttgacttgg tggttccatc tgccagcttt
    12361 ctagaacgcc agttgactgc gggagttttc cagccgctgg acaaaagcaa attgccggag
    12421 tggaagaatc tcgacccgga gctgctgaag ctggtcgcca aacacgatcc cgacaataaa
    12481 tttgctatgc cctatatgtg ggcgacgacc gggattggct ataacgttga taaagttaaa
    12541 gctgtgctgg gcgaaaacgc gcccgtcgat agctgggact tgatcctcaa acctgaaaat
    12601 ctggaaaaac tgaaaagctg cggtgtctct ttcctggatg cgccagaaga agtttttgct
    12661 accgtgttga attatctcgg caaagatccc aacagcacta aagcggatga ttacaccgga
    12721 ccggcaaccg atctgctgtt aaagctgcgc ccgaacattc gttatttcca ttcatctcaa
    12781 tacattaacg acctggcaaa cggcgatatt tgcgtcgcta tcggctgggc aggtgatgtc
    12841 tggcaggcgt caaaccgcgc gaaggaagcg aagaatggag tgaatgtctc gttctcgatt
    12901 ccaaaagaag gggcgatggc gttctttgat gtattcgcca tgccagcgga tgccaaaaac
    12961 aaagacgaag cctatcagtt cctgaattac ctgctgcgcc cggatgtagt agcgcatatt
    13021 tccgaccatg tgttctatgt caacgccaat aaagcagcca cgccgctggt gagtgcggaa
    13081 gtccatgaga acccgggtat ttatccgcct gcggatgttc gtgcgaagct gttcactctg
    13141 aaagtgcagg acccgaaaat cgaccgtgtg cgcacccgcg cgtggaccaa agtgaagagc
    13201 ggaaaataat ccgcagcctt agatgccggg cgggcgcacc acacccgccg gcaattcgca
    13261 ccatcatggt gcgcttgcac acattcaatg ccggagagca gccgtgaatg acgctatccc
    13321 tcgcccgcag gcgaaaaccc gtaaggcgct gacgccgcta ttagaaatcc gcaacctcac
    13381 caaatcctac gatggtcaac atgcggtgga tgatgtcagc ctgaccatct acaaaggtga
    13441 aatcttcgcg ctgctgggcg catccggctg tggcaagtcc acgctgctgc gtatgctggc
    13501 aggtttcgaa caaccttctg ccggacagat aatgcttgat ggcgtcgatt tgtcacaggt
    13561 tccgccttac ctgcgcccca tcaatatgat gtttcagtct tacgcgctgt ttccgcatat
    13621 gaccgtggaa cagaacatcg cttttggcct gaaacaggac aaactaccga aagcggaaat
    13681 ttccagtcgg gtcaatgaga tgctcggcct ggtgcacatg caggagttcg ccaaacgtaa
    13741 accgcatcag ctttctggtg gtcagcgaca gcgtgtggcc ctggcccgaa gccttgccaa
    13801 gcgcccgaaa ctattactgc tcgacgagcc gatgggcgcg ctggataaaa agctgcgtga
    13861 ccggatgcag cttgaagtgg tggatattct ggagcgcgtc ggtgtgactt gtgtgatggt
    13921 cacccacgat caggaagagg cgatgaccat ggcggggcgc atcgccatta tgaatcgtgg
    13981 gaaatttgtc cagattggcg aaccggaaga gatctacgag catccgacta cccgctatag
    14041 cgccgaattt attggctcgg taaacgtctt tgaaggcgta ctcaaagagc gtcaggaaga
    14101 tggcttggtg cttgattcgc cggggctggt gcatccactg aaagtcgacg cggatgcctc
    14161 ggtggtcgat aacgtgccgg tacatgtggc gctgcgcccg gaaaaaatca tgctttgcga
    14221 agagccgccc gccaatggtt gtaacttcgc ggtgggggag gtgatacaca ttgcctatct
    14281 cggcgatctt tcggtgtatc acgttcgtct gaaaagtggg cagatgatta gtgcccagct
    14341 acaaaacgcc catcgtcatc gtaaagggtt accgacctgg ggcgacgaag tgcgtttgtg
    14401 ctgggaagtg gacagctgtg tggtgctgac ggtttaagga gcaaagatga atacacttga
    14461 acctgctgcc cagtcgaaac cgccgggcgg atttaagctg tggttgtcgc agctgcaaat
    14521 gaagcatggg cgcaaactgg tcattgcgtt gccatatatc tggttgatct tgctgtttct
    14581 gctgccattt ctgattgtct ttaaaataag cctggcggag atggcgcgcg ctattccacc
    14641 ttataccgaa ctgatggagt gggctgacgg gcaactgtcc atcactctta atctcggtaa
    14701 ttttctgcaa ctgaccgacg atccgctcta tttcgatgct tatctccagt cgctacaggt
    14761 ggctgcgatt tcgacatttt gctgtttact gatcggctat ccgctggcgt gggcggtggc
    14821 gcacagtaag ccttcgaccc gtaatatttt attactactg gtgatcctgc cgtcgtggac
    14881 ctcgtttctg atccgcgttt atgcttggat gggaatatta aaaaacaacg gtgtgctgaa
    14941 taattttctg ctgtggctgg gggttatcga tcaaccgctg accattcttc ataccaatct
    15001 ggcagtttat attggcattg tttacgccta cgtgccgttt atggtgctgc cgatttatac
    15061 cgcgttgatt cgtattgatt attcgctggt ggaagcagcg ctggatctcg gtgcacgacc
    15121 gctgaaaacg ttctttactg tgatcgtgcc gctgactaaa ggtgggatta ttgccggatc
    15181 gatgctggtg tttatcccgg ctgtgggcga gtttgtgatc ccggaactgc tcggtggccc
    15241 ggacagcatc atgatcgggc gcgtgctatg gcaagagttt ttcaataacc gcgactggcc
    15301 ggtggcctcg gcggtagcga tcatcatgtt gctgctgcta attgtgccga taatgtggtt
    15361 ccacaaacac cagcaaaaaa gcgtgggaga acacggatga ataatttacc ggtagttcgt
    15421 tcgccctggc ggattgtgat tttgctgctg ggctttacct ttctctacgc gccaatgctg
    15481 atgctggtta tctattcgtt taacagctcg aagctggtga cggtgtgggg cggctggtca
    15541 acgcgctggt atggtgagtt attgcgcgat gacgcgatga tgagtgcggt tggttcaagc
    15601 ctgacaattg ctgcctgtgc ggcaacggcg gcggcgatcc tcgggactat tgcggcggtg
    15661 gtgctggtgc gctttggcag gtttcgcgga tcaaatggct ttgcctttat gatcaccgcg
    15721 ccgctagtaa tgccagatgt catcacgggc ttgtcgctgt tgttattatt cgtcgcgctt
    15781 gctcatgcca ttggctggcc tgcggaccgc ggtatgctta ccatctggct ggcgcatgtc
    15841 actttctgta cggcttatgt ggcggtcgtt atttcgtcgc gtctgcggga actggatcgc
    15901 tcgatagaag aagccgcgat ggatctcggt gcgacgccgc tgaaagtgtt tttcgtcatt
    15961 acgctaccga tgatcatgcc cgcgatcatt tctggctggt tactggcttt tactttgtcg
    16021 cttgatgatc tggtgatcgc cagctttgtt tccgggccgg gagccaccac gttaccgatg
    16081 ctggtctttt ccagcgtgcg gatgggggtg aatccggaaa tcaacgccct ggcaacattg
    16141 atcctcggcg cggtcggaat tgtcggattt atcgcctggt atctgatggc gcgcgcagaa
    16201 aaacagcgga tacgcgacat ccaacgtgca agacgtggct gaagacacta aaatttgcca
    16261 acctggctac ataatgccgc gcatgtcgcg gcattgtttt catggaagac gaaacgttgg
    16321 gattttttaa gaaaacatct tcatctcatg ctcgcctgaa tgtgcctgcg ctggtgcagg
    16381 tggcggcgct cgccattatt atgatccgtg gcctcgacgt gctgatgatt ttcaatacgc
    16441 tgggcgtgcg cggtattggc gagttcattc atcgcagcgt acaaacctgg agtttaacgc
    16501 tggtcttttt aagcagtctg gtgctggttt tcattgagat ctggtgtgcg ttttcactgg
    16561 tgaaagggcg tcgctgggcg cgctggctat atctgctgac acaaatcacc gccgcaagtt
    16621 acttgtgggc ggcttcgctg gggtacggtt atccggagct gttcagcatt cccggtgaat
    16681 caaaacgtga aatcttccat agcctgatgc tgcagaagct gccggatatg ctcatcctga
    16741 tgctgctgtt cgttccctcg accagtcggc ggttcttcca gttgcaataa tgtgtataat
    16801 cgtcgcccct gatgatgtga aggtcaatgt atgcagtgcg cactttacga cgcgggtcgc
    16861 tgtcgttcct gtcagtggat aacgcagccg attccagagc aactctccgc taaaaccgcc
    16921 gatcttaaaa atctgctcgc cgattttccg gttgaggaat ggtgcgcgcc ggtgtcaggc
    16981 ccggaacaag ggtttcgtaa taaagccaaa atggtggtga gtggtagcgt tgaaaaaaca
    17041 ctgctcggta tgctgcatcg cgatggcacg ccggaagacc tttgtgactg cccgctttat
    17101 ccagcctcat ttgcgcccgt ttttgcggcg ctaaaaccgt ttatcgcccg agcggggtta
    17161 acgccctaca acgtggcgcg taagcgtggc gaactgaaat acattctgct gactgaaagc
    17221 cagagtgatg gcggcatgat gctgcgcttt gtactgcgtt ctgataccaa gctggcgcaa
    17281 ctgcgtaagg cgctgccgtg gttacacgaa caactaccgc agctgaaagt tattaccgtc
    17341 aatattcagc cggtacatat ggcgattatg gaaggggaga cggagatcta cctgaccgaa
    17401 caacaggcac tggcggagcg ttttaatgac gtaccgctgt ggatccgtcc gcaaagtttc
    17461 ttccagacta atccggcggt cgccagccag ttgtacgcca ccgcgcgcga ctgggtacga
    17521 cagctgccgg ttaaacatat gtgggatctg ttctgcggtg tggggggctt tggtttacac
    17581 tgcgcgacgc ctgacatgca gttaaccggg atcgaaattg cgccagaggc cattgcctgt
    17641 gcaaagcagt cagccgctga actgggctta actcgtttac aatttcaggc gctggactcc
    17701 actcagtttg ccactgctca gggggaggtg ccggagctgg tgctggttaa cccgccgcgc
    17761 cgcggcattg gtaaaccgct gtgtgattat ctttcaacga tggcaccgcg ttttattatc
    17821 tactccagct gtaacgccca aaccatggcg aaagatatcc gcgaactgcc aggttaccgc
    17881 atcgaacggg tacagctttt tgatatgttc ccgcacaccg cgcactatga agtgttgacg
    17941 ctgctggtga agcaataaaa aagccacagg tgcggcttca gattgctgat caggtgcgct
    18001 ttgttcatgc cggatgcggc gtgaacgcct tatccggcct acaaaatcat gctaaatcaa
    18061 tacattgcag agttgatgtg ggccggataa gcgcagcgca tcaggcaatc ttacttttgt
    18121 catcagtctc aattactgcg gaaaccactg gtcattgatt ttctgatatg taccgtcagc
    18181 tttaattgct gccagcgcgt tattcagttt ttccagcaga gctttgttat ccggacgtac
    18241 agcgataccc aggccggtgc cgaagtattg cggatcggtc actttttcag tcgcaacgcc
    18301 cagttgcgga ttggttttca gccattcgtt taccaccgcg gtgtcaccaa ataccccatc
    18361 aatacgacca tttttcagat cgataaaggc attctgataa ctgtcatagg agacagtttt
    18421 cacttccggg tgctgatcct gaatatattt ctggtgcgtc gtaccgtttt ccatcccaat
    18481 gcgtttgcct ttcaggtcgg caaacgtttt gtaggtatct tttttggaaa tcacgacggc
    18541 tgagttttca tagtagggtg tggtaaacga aacctgcttg ctacgctccg gggtgatatc
    18601 cataccggag attacggcgt catattttct gaatttcagt gacgggatca ggctgtcgaa
    18661 cgcgtgatta gtaaaagtac attctgcctg catttgtttg cacaaggctt ttgccagatc
    18721 gatatcaaag ccgacaatct cattattagc acctatagat tcaaagggtg gataggtggc
    18781 tgaaacgcca aaattgattt tctctgcggc agaagcaccg aaagtaaagg aagcaagtaa
    18841 agcggcaaga actaactttt tcatgatgga actcccgtct gtcaatctta tgatttttgg
    18901 ccgtgtctgc ggcatgggat aacaatgcca tcaagtgaat ttatatgcaa taaacatgat
    18961 taaataattt aaatgaaata aaaaaagacg gataacttag tgagttgtcc gtcttcatta
    19021 taagaattta tgcactatgt aggccggata aggcgtccac gccgcatccg gcacaggcac
    19081 cgtgctgatg tctgatgcga cgctggcgcg tcttatcaga cctacaaacc cccccggcga
    19141 atgtacgcag ctacattaat tccgccgttc gaatgccagt gctttgcgct cgatcagacg
    19201 catcatcagc gtcagcaggc cgttgacgac caagtaaata atccctgccg caccgaacac
    19261 cattacatcg taggtgcgtc cgtacaacaa ctggctgtat cccatcactt ccatcagcgt
    19321 aatggtgtaa gccagagagg tacttttgaa caccagcacc acttcgttgg aataagaaga
    19381 gagcgagcgt ttaaaggcat acggcagcag gatcgccagc gtatcttttt tgctcattcc
    19441 cagggcgcta caggactgcc actgaccttc tgggatcgca cgaattgcac cgtaaaacag
    19501 ctgcgtggta tacgccgcac tgttcagcga taacgcaatc agcgcacata accacggttc
    19561 tgacaacaaa tgccacagtg ccggatactc ctgcaacgtc ggaaactggc ccggcccgta
    19621 ataaatcagg aagatctgca ccagcagcgg cgtaccggta aacagcgtga tataaccccg
    19681 caccagccac accagcaccg gcgttttcag cgtcaggatg atggtaaaaa tcaatgccag
    19741 aatcagtgcc acaatcagcg aggcaacggt tagcgtcagg ctggtgtgca gccctttcat
    19801 cagctcgggt aaatactcaa acattagctg ggcctccgct caaaacgtgt cgcgcgcagg
    19861 tcaatgcgtt tgagaatgta ctgactgagc agggtgatca ccaggtaaat cgccgccgcc
    19921 acaatgtacc aggtaaatgg ttcctgggta cgagtagcaa tgcttttggt ttgtaacatt
    19981 aaatcattca cactaatcaa actgaccagc gcggtatctt tcagcagcac cagccactgg
    20041 ttaccgaggc caggcagcgc atgacgccac atctgcggca tcaccagacg gaaaaagata
    20101 gccgatttcg acagccccag cgcctgacca gattcccact ggcccaccgg tacagctttc
    20161 aacgcgccgc gcagcgtttg cgaggcatag gcggcataca gcagtgacag agcgatgaca
    20221 ccacaaagga acggactcac gtcgaagttc tcaatgtcca tctgcaccgg gatctgcacg
    20281 aacccaagat tgatagtgaa gccatccgaa agcgtcagca gtaactgcga ggagccaaaa
    20341 tagataaaca gcactaccag aatttctggc aggccacgca gaatggttac cagcgctgaa
    20401 cctgcccacg cgacaggacg ccatttcgct gactcccata ccgcaaagaa catcgccagc
    20461 gccagcccga caatcaatgc acaaacggca aggccgacgg tcatcccggc ggcgcttgct
    20521 aaaggaaaaa attcattcat caggaattac ttctggaacc atttgttgta gatggtttcg
    20581 taagtgccat ctttcttcac tttttccagc gcagtgttga gtttctgctg cagttcagtg
    20641 tttccctgac gtaccgcgat gccgaggcca gtaccgaaat aatctttatc ggtcacttta
    20701 tcgcccactg ccgccagttt tgggttatct ttcagccact cagtgaccac tgcggtgtca
    20761 ccaaatacgc tgtcgatacg cccgttttgc agatccagtt ttgcgttctg gtagctgtca
    20821 tacggaacgg tggtgatttc cgggtgctta tccataatga acttctggtg tgtcgtcccg
    20881 ttctgaacgc cgactttttt gcctttcagc tgatcaacac tggtgtattt gccttgctga
    20941 cccacaaaca gggcagagtt gtcatagtac ggggtagtaa acagtacctg cttttcacgc
    21001 tccggagtga tatccatgcc cgccatcact gcttctacgc gacggaattt caggcttggg
    21061 atcaggctgt caaacgcctg gttagagaaa gtacaggttg catcaatctc tttacacagt
    21121 gcctgcgcca ggtcgacgtc aaaaccaacg atctggttgt ttgcatcaat cgattcaaac
    21181 ggaggatagg gggcttcggt agcaaaacga atggtttcgg cagctgtggc ggaaagacta
    21241 aaacctgcaa ttaacgcggc aatcagaact tttttcattg ttgttatccc gaatcttagt
    21301 gagagagata gtttttaaat gcttcggttt gcggctcggt aaagcagctc gcgtcgcctt
    21361 gttctacgat atgaccattt tccatataca ccactcggct ggcggtttta cgcgccactt
    21421 caacttcgtg ggtgacgatc acctgggtaa tattcgtttc tgccagctca cgaatgatgc
    21481 tgacgatttg tgccgtaatt tccgggtcca gtgcggcggt cggttcatcg aacagcagta
    21541 cctgcggttc catcatcaac gcacgggcaa tagcaacacg ctgctgctga ccaccagaaa
    21601 gatgcagcgg gtaacgatcg ctataaggtt tgagacgcag acgttccagc agtttttctg
    21661 cacgggccag cgcctgatct ttactcaacc ccagtacacg gcagggcgct tcaatcaggt
    21721 tttgctgcac ggtcagatgc ggccacaggt tgtattgctg aaacaccatg ccaacgttac
    21781 gacgcaaatc gcgaatcgct ttgtcagagg gtgttttggt gaaatcgaaa tggttgcctg
    21841 caatgttgag cgtaccggag cgcggcatct caagcagatt gagtacacgc agcagcgagc
    21901 ttttacccgc gccgctgggg ccaagtaaca ccagcgtttc gccctgtggg caatccagcg
    21961 tgatatcgaa cagcgcctga tgcgcgccgt agaagcaatt aatgccgttt aattgaatac
    22021 tcattgacac tcgtatactg gcagtctgat agctattgag gtcgaagata gtacctttaa
    22081 cagaataatt atgcaatatt tatgctttaa aagttaaaag caaagcgcat tattcaataa
    22141 acatagcaca aaataacggg ggcggtggtc ggtgagcata aatgtcggca ttcctcacga
    22201 aatgccggat aatttacggg gtttattggt tgatcaaggc gttagcgatt ctcgatggac
    22261 tgacggagcg tacccgccgt ggcatgaacg ctaccgccta agtagcgcac atcgtcgatg
    22321 acccaacact ggccttcctg aatcattaac acttcatcct gccagccctg gtcaccctgt
    22381 ttgagatcca cccgcaatgg aatgttacgg gcatcacgat tagggatagt cgatgcactg
    22441 gcaacgtggg cgctgtctgg caaggtggtt cgactggaga acggatcgct gcttaacaat
    22501 tcacgatgac tattatcgcg gctggcatcg ctaagttggg ttgccagttt gtcactcaaa
    22561 taggggcgca gggcggtaat gtcattgctg cgatgttgaa tgcggtagtc gtagaattgt
    22621 tgagccacac tgtcaggccc tccctggaca caggaaccgg tgcgggggcc gttatcttta
    22681 taagctggcg tgacggtggt acaggcactc agcagcagtg cacatggaat aagtagagat
    22741 agtgaacggt agcgcataat catttcctta ttagcttgct gtctgcgtca cactacgacc
    22801 aaacgctatc agattattta atacattgtt tttaatacaa ttctgaaaca atctgaacac
    22861 ctgattgggt ttgagtccat tgctggcgac ccactcaagg ttgctcgtag ctgacttact
    22921 taaattaccc aaacaataga cagtgtaaac catttgtgag gttgagcgtg ttatatggct
    22981 gaaatgatca tttaaggtaa atatgatagt tctgaaatgg tggtataaat cctcctgtag
    23041 aggttaagag catctgcaag aagccagttt cacatttata acgcgtgata aatgaatgcg
    23101 gttattgtct aatcattaca aaatttttca catgcgataa gagatcctgc gattaagatg
    23161 attgccaaaa ttacgttgta tttttgaaaa tgagcgaaat ttgatagtcc cagctttgac
    23221 tctcgataat attatttatg ctacaattgt gggtaaagaa tttaagctaa tgaatttgag
    23281 tgaagaataa attaaattgc ttcagtattt cgttaattaa tataaataca aaaggcgttg
    23341 tgcttttata attataaagc gcgaggagtt cggtggatta tcgtttttct tgggtgattt
    23401 ttaatatgat aaacaatata tctgatcaag catcatcatt tccaggcaca caactaaatc
    23461 aaagtgataa tttcctggat tcactgaggg aatttttcgc gatattaaat cccagtcgta
    23521 agggtgagtt gtctacatgg gataccatct acttgcatct gatattagca ataaatgctg
    23581 acagtgatct cataaaaaat gatgtgttgc tcgcggaaaa tattccatca gcgaattacc
    23641 aatttaatac attttttagc aatactttcg agattgatgt taaaaaatat ctgggtaaat
    23701 cagaagataa cgaagttgaa ataaaagcgg gtaatgaaag aatctctatt ggaatacgta
    23761 atgtatcgaa tggaaaactt gaaagacaac agtttctttt cccactggac tatgagaata
    23821 aattacaaga acaattagat aagtatttta cgattgaatc gcacccatta ttatacaggt
    23881 ataccattgg tagtaagatt gcgaatgtga tttttgaaaa gttatacagt cgtattgact
    23941 ttaacaaaga acaatatata tcctttataa aggacgcatt tattcatttt tatgattata
    24001 gcaggcgata tgctattagt gagaatatcg ataaagacgc tgtaacaaac aatatcgcat
    24061 tgatgagcac tttttatgac agtgataata cgtcaggtga ggttttaaat aatgatttta
    24121 cagaagaaga gtcatttgaa actgcgttag atgtagagca tgcaatagtg cttggatttg
    24181 ctgatgataa ttttgaaaca aaacctgttc attatcagga tttattaaca cgatttagtg
    24241 cctttcaaga taccgtgttt aatctgtttc ctgaaatgca ctcttcgcat taccacgata
    24301 tttgtagcgt aagtgtggat atgacaaagg gaacgcaatg catgattcac ttgatggtga
    24361 atgaagaagt tttcatgtct ctgcctgttc cagttgccac gatggtacgt gaagatgcaa
    24421 gtaatttagt caatcttaaa acactactca atgatggttg tttcattaaa tatagtcatt
    24481 ttaatgacgt ggcattaata aagcaaaaca taagtaatct ctatttatca cacactgttg
    24541 tgaatgaaag catattgaaa aaatgttgtt ttgaaaacgg cagtttaggt gatgttaaaa
    24601 taacaaatag caatgtaata aacagtgcat tcaaaaatat ttcgtttaga tctgttaaaa
    24661 ttaataacgt aaatactcat tctttaaaat ttattaactg caattttttc aatgttgata
    24721 tgataagagt taatctcagc aaatgtcttt ttcatgaatg tagtatgcat ggtgtcaaga
    24781 taaaaccctg gttgcctgta aaatggacaa aagaattaat aagtgattat ttgtatggct
    24841 gtttactatc tctgtactct atctgtgcaa gggatatata taatatgaac gccgggaata
    24901 acgtgaaggt tgctgcggat gctttccttg aaattatttt ctctcttaaa aataaatatt
    24961 gtattaaact attaagcgct caggatcggg cgtttatata cgagtttgca agaatgatct
    25021 tcgcttatat taatgacaaa agcattgaga tcttattact ttcttgtttt gcggcggctg
    25081 accaaaaagc aatccagcgg tatagacctc agtcacaaga tggggaagac tttaggagtc
    25141 atttacaata taagttgcct ctttccgctc attaatgttg ttggtgcaaa ataataagac
    25201 aggtaagcgg taccctataa tgaaaccaat atcgggttat gcaacggata atcgtaaaga
    25261 cttaagtggg tgtttttgac acaatgagat gatttcctaa actgatcaaa accttcttcc
    25321 caacatggca tatcagtccg ataatgcttt agagatcgaa ggcttaacaa acaaggagat
    25381 cgatcatgca attttctaca accccaactc tggaaggcct gaccatcgtt gaatattgcg
    25441 gtgtggtgac cggcgaagcg attttaggtg ccaatatttt ccgtgatttc tttgccggta
    25501 tccgcgatat cgttggcgga cgttccggtg cgtatgaaaa agaactgcgt aaagcacggg
    25561 agatcgcctt tgaggaatta ggctcccagg cgcgggcgct gggggccgat gccgtagttg
    25621 gtattgatat cgactacgaa acggtcgggc aaaacggcag tatgctgatg gttagcgtca
    25681 gcggtacggc ggtgaaaacg cgtcgatgag aagagtcttc tggctggtcg ctgccgctct
    25741 gttactggca gggtgtgcag gcgaaaaagg cattgtcgag aaagagggat atcagcttga
    25801 tacccgacac caggcgcagg cggcgtatcc gcgcattaaa gtgctggtga tccactacac
    25861 cgcagatgat tttgatagct cgctggcgac actgactgat aagcaggtca gctcgcatta
    25921 tctgatccct gcggtgccac cgcgatacaa cggtaaaccg cgcatctggc aactggtgcc
    25981 ggaacaagaa ctggcctggc atgcggggat tagcgcctgg cgcggggcaa cgcgccttaa
    26041 cgacacctct attggcattg agctggaaaa ccgtggctgg caaaaatcat ccggagtgaa
    26101 atattttgcc ccgtttgaac aggcacagat tcaggcgctt attccgctgg cgaaagatat
    26161 tattgcccgt tatcacatca agccggaaaa cgtagtggca catgcggata tcgcaccgca
    26221 gcgcaaagac gatccggggc cattatttcc ctggcagcaa ctggcgcagc aggggattgg
    26281 tgcctggccg gatgcgcagc gggtgaactt ttaccttgcc gggcgcgcgc cgcacacacc
    26341 ggtagatacc gcgtcattgc tggagctttt ggcgcgctac ggttatgacg taaaacctga
    26401 tatgacaccg cgcgagcagc ggcgcgtgat tatggcattc cagatgcatt tccgcccgac
    26461 gttatataac ggcgaagcgg atgcagaaac tcaggcgatt gccgaagcat tgctggagaa
    26521 atacgggcag gattagcgcg gcagttttcc gtggtcgcgt agccaggcgg cagttttctc
    26581 gatgccttca tccagggtga tgaccggctg ataacctaac tcttcctgcg cacgcgtaat
    26641 atccagcgta aagtcaaaat tcaacttgga ggcgccgtag tgggtcagcg gcggctcttt
    26701 tgctgacttg cggcctaaac gctccatgct gcgggcgatc atatccagca tcgggtaagg
    26761 gacggaacga atacgacagt caatattcaa ctcgtcgatc agcttctgca cgatgctgcg
    26821 cagtgtgcga tgctcgccgt tggtgatgtt gtacacacgc ccggaaggta gcttatcgca
    26881 ggcttcctgg cttgccagcc acattgcgtg cacggcattt tcatagtagg tcatatcgac
    26941 cagcgcactg ccgccatgcg gtaacagaat actgccgtag tggtgcatca tatgcgccag
    27001 acggggaata aagactttat cgtgcggtcc gaacagactt tgtgggcgca gaatagtaaa
    27061 gcgcgtttgt ggattcgcct gcgaaagcat attgatcact tcttcgctgg ctgctttgct
    27121 gcgggcaaac tcgttggcga agcggtgagg gcgaaaatct tctttaatat tgcgatggtg
    27181 gtgataatcg aagtacaggg agggggaaga gatatgaata aagttacgca caccccaggc
    27241 gacagcccat tcacccaggc ggcgagtggc gcgaacgtta gccagatcga aagcctgttg
    27301 tgtcccccag ggtgaggtaa agctggagca gtgccacagc gtatcaatgc ccgcgagcat
    27361 cactttagct tgtgatgaaa ccagctcggt cagatccgtc ggaacaaact ctgcgcccat
    27421 tttttccagc aatttaccca ttgcttcgtt gcgaccgctc gcacgcacgc tgatgccttt
    27481 ctggcataaa aactctaccg cgtttcgacc taagccgctg gtggcgccgg taaccagtac
    27541 cttcatatca atccactgtt gttgagaaaa tgacgtgcgc attcttccgt gatttccccc
    27601 atgatgcaat gggaaacatg aaagaataac gcaggttttg tcgattaatc tgtgctttgt
    27661 tctgccagtc tggcgatttg ttttgccatt ccgcgaaaaa taaacagatg cgcggggatc
    27721 atcaataacc agtaaaacag ccccggcata ccgtgcggat gccagaaagc gcggacatcg
    27781 atagtacgat agtcaccttt atcttccagg gtaaaacaca gtcgtcccag ccccggcgct
    27841 ttcatgccaa ataacaacgt aagttgcttt ttcggttcaa cgacaatcac tttccagcta
    27901 tccaccgcat cgccggtctg taaatattcg cgctccgggc ggcctttcgc cagattatga
    27961 ccgatcgcgc ggtccatcaa cgcccgtgtc tgccacaaaa tattgccaaa gaaataacgc
    28021 tctttaccgc cgatttggtt caccaccagc cataaagccg caaggctggc ggacgtttta
    28081 acggtaaacc ccgcctgttt ggcgaaataa ccgtactccg gtcgccagcg ggcaaaggcc
    28141 tgagcgtcgt agccccagtc gctggagttg accagttttt cttcctcttt caacgtgcta
    28201 cgtaccgcgt catcgaaagc gatcagccgt tgtgggatga gtgcacgtag cgcggtatca
    28261 tctgccagca gatcgtgttt cagcccctga atcaacgccc tggcggtggt gggcggtacg
    28321 gaagtaatca cattgagaaa ccacaccgaa atccagcggg tggggagggg gatggggatc
    28381 aaccagcggc gcttaccgct caccgccata aaatgttcaa actgttgctg ataactgagc
    28441 acctctggtc cggcggcttc gaagatgcgg tgttcgttgg ctggatggtc taacagcgcc
    28501 accagatagt gcagcaagtt ttccagcgcg atgggcgtgg tgcgtgaacg tacccagcgt
    28561 ggcggcgtta acactggcag gttgtagacc atatcgcgca tgacttcgaa cgccgctgaa
    28621 cctgcgccaa cgataattcc ggcccgaagt tcggtcactg gtacattcgc ttcacgaaga
    28681 atgtccgccg tagcctgacg agctcgcaga tgatccgact gctcatgtgg cggggcctgc
    28741 aacgaactga gaaagattaa ttgcttaact ggtacttcac gtagcgcatc gcggacgttg
    28801 agagccagct ggcgctcctg agcgataaaa tcgccgcctt cgcccatgct gtgcaccaga
    28861 aaatagacgg tatcgatatc ctgcaacagg gccggaaggt tatccggcca gctgagatcg
    28921 actttatggc aactgacgtt tgccagttgc agctttgcaa gcctgtcgac atgacgtgcc
    28981 gccgctagga tctgatgccc ttgctggctg agtgtgcgca ccagatgctg accaatgtag
    29041 ccactggcac cgagaactaa aatgcgttgc ggcacgtctc tctccttaac gcgccaggaa
    29101 tgcgcgccag tgggcggcga cttccgccag ttgttcgcgc gagacgtcaa gatgcgtcac
    29161 caggcggaca atcggcgagg cgttaatcag cacgtttctc gctttcatgt attcgcctaa
    29221 cgcggcagca ttttcttccc cgacgcgaac aaacagcata ttagtgtcct gacgcatcac
    29281 atccgcgcct gcttcacgca gttgctccgc catccaggcg gcgttgtcgt gatcttcctg
    29341 caaccgtgcg acgttatttt ccagggcata catcccggcg gcagccagaa tcccggactg
    29401 acgcatcccg ccaccggcca ttttccgcca gcggatggca cgtttaatgt aatcacgatt
    29461 accgacgagt aatgaaccga ctggcgtccc aagacctttc gacaggcaaa tggtgaacga
    29521 atcacaatat tgcgtgatct ctttcagttc gcagccgtaa gccaccacgg cattaaagat
    29581 gcgcgcaccg tcaacatgca gcgccagatt gcgctcgcgg gtaaattccc atgcttcttt
    29641 caggtattca cgcggcagca ctttgccgtt gtgggtgttt tccagactga gtaatttggt
    29701 gcgggcgaaa tggatatcgt cgggtttgat tttcatcgcc actttatcca gcggtagcgt
    29761 gccgtcggca gccgcgtcga tgggttgcgg ctgaatactg cccagcaccg ctgcgccacc
    29821 ggcttcaaac agatagttat gcgcggcctg accgacaata tactcttcgc cacgttcgca
    29881 gtgactgagc agagcgacca ggttggcctg agtgccggta ggcagaaaaa tggcggcttc
    29941 tttaccggaa agctctgctg catagtcctg cagagcatta acggtagggt cgtctccgta
    30001 aacgtcgtcc ccaaccgggg cggccatcat cgcttcgagc atggcgcggc tcggtcgggt
    30061 aacggtatca ctgcgtaaat caatcatggc atgtccttat tatgacggga aatgccaccc
    30121 tttttacctt agccagttcg ttttcgccag ttcgatcact tcatcgccgc gtccgctgat
    30181 aattgcgcgc aacatataca ggctaaaacc tttggcctgt tcgagtttga tctgcggtgg
    30241 aatggctaat tcttctttgg cgaccaccac atccaccaac accggaccgt cgatagagaa
    30301 ggcgcgttgc agggcttcat caacttcaga ggctttttct acacggatac ccgtaatgcc
    30361 gcacgcttcg gcaatgcggg caaagttggt gtcgtgcagc tcagtaccgt ctgtcaggta
    30421 acctccggcc ttcatctcca tcgccacaaa gcccagcacg ctgttattaa agacgataat
    30481 tttcaccggc agtttcatct gcactactga gaggaaatcg cccatcaaca tgctaaaacc
    30541 gccatcgccg cacatggcga ccacctgacg ttccggctcg gttgcctgcg cacccaacgc
    30601 ctgcggcatg gcgttagcca tcgaaccgtg gttaaacgaa cctaacaggc gacgcttgcc
    30661 gttcattttc agataacgtg ccgcccatac cgttggcgtg ccgacgtcac aggtaaagat
    30721 ggcgtcatcg gcggcaaaat gactaatttg ctgcgccaga tattgcgggt gaatggcttt
    30781 ctcgctcggt ttagctaaat cgtccagccc tttgcgggca tcgcggtaat cttccagccc
    30841 tttgcgggca tcgcggtaat cttccagcgc tttatccaga aacttgcgat cggttttttc
    30901 ttccaccagt ggcagcaatg cgcgcagggt tgatttgata tcgccgacca gcgccatatc
    30961 taccttgcta tgcgcgccga tgctggctgg attaatatca atctgaataa ttttggcatc
    31021 ggtcgggtag aacgcacggt aggggaactg agtaccgagt agcactaacg tgtcggcgtt
    31081 catcatggta tggaaacctg acgagaatcc gattaacccg gtcattccaa catcatacgg
    31141 attatcgtat tcgacatgct ctttaccgcg tagggcatga acaataggtg ctttaatttt
    31201 cccggcaaac tcaactaact ctttatgcgc ccccgcgcag ccgctgccac acatcagggc
    31261 gatattgctg gaataacgca gcagttgcgc cagtttgcgt aactcttctt cttccggcat
    31321 cacgactggc tgtggcgcat gataccagtg cgtggttgcc ccttctggcg caggttttaa
    31381 cgccacgtcg cccggtaata caacaacgga aacgccacgg ttaagcaccg ctttgcgcat
    31441 agcaattgcc agcacttgtg ggatctgctc cgggctggaa accagctcgc aatagtgact
    31501 acattcgcgg aatagctctt gtgggtgggt ttcctggaaa tagccgctgc caatttcgct
    31561 ggagggaata tgagcggcaa tcgccagtac cggaacgtta ttgcggtggc aatcgaatag
    31621 gccgttgatc aagtgcaggt tgcctgggcc gcacgatccg gcacagaccg ccagttctcc
    31681 tgaaagttgt gcttcagcgc cagcggcaaa cgccgccact tcttcatggc gggtggacat
    31741 ccactcgatg gtgcccatgc gattaagact gtcgctaaga ccgttcagag agtcgcccgt
    31801 gactccccag atgcgtttca cgcctgccga ttcgagtgtt ttggcgatat aagctgcaac
    31861 cgtttgtttc atggttctcc atctcctgaa tgtgataacg gtaacaagtt tagttcatct
    31921 gacggagggg gaagggatgg gagagaaagg aggcactaac ggttaaatag cccgatgaaa
    31981 ggaatatcat cgggcataag gcgattatgc gagaaccaaa tccccctgcg gatggcagga
    32041 gcaggccagt acgtaacctt cagcgatttc ggcgtcggtc agcgtcattg tactgctcac
    32101 cgtatattca ccggaaacca cttttgtctt acagcagccg caaacacccg cacggcaggc
    32161 agcgacaacc ggaacgttat tgctttccag cgcctccagt agcgtggtgc caaccggggc
    32221 gtaaaattct cgtgccggtt gcagtttggt gaatttcaga ccgctggtcg ccgcttctgc
    32281 tactggggtg aagaatttct ctttaaagaa acgcgtcacg ccgagcgctt tcacttcctg
    32341 ctctacccaa tccatatacg gagccgggcc gcaggtcatc acggtacgtg aagctaagtc
    32401 aggtacatct gccagcagtt cgcgagtgag gcgaccagcg ataaagcctt cggtaacgtt
    32461 attttctgcc accagcgtta ccggatagtt acgccactca tcggcgaaaa taacatcctg
    32521 cggcgtacgc acgttgtaga tcacccgcac atcggcctgt ggacggttct tcgcaagcca
    32581 gcgacgcatc gacataatcg gcgtgacgcc gcagcctgcc gccagcaaca ggaatttatc
    32641 ttctgcttta tcgtcgcagg taaattcccc catcgcgtcc gaaagccaga gataatcacc
    32701 gcgttttaca tcgcgcgtca gccactggga gccgacaccg tcatcaatcc gccgcacggt
    32761 cagggtgata tattcactca cgcctggcgt ggaggaaatg gtgtaagcac gcagcgtttc
    32821 cgctgagttt cgcacgctga ccagtgcata ttgcccggcg cgatatgggt agtgatcgtg
    32881 gcaaatcagg gaaatcgtcc acacatccgg cgtttcttgc gtaatgtgat gaacctgtat
    32941 ccgccacggg cattgattcg ttggcatcgt catcgacaaa ctccttacgc gttcaacagt
    33001 tgcttcatgt cttcttcaac agtggtgata gaacgcaggc cgaatttctc gttcagcacc
    33061 gccagcaggt ctggtgtcag gaaaccaggt gcagtcgggc cggtgacgat atttttcacg
    33121 cccagagaaa gcagcgtcag cagaatgaca atcgctttct gttcaaacca ggagagcacc
    33181 agcgacagcg gcagatcgtt cacaccacag cccagtttct ctgccagagt gacagccaga
    33241 ataattgctg agtaagcatc gttacattga cctgcatcta ccagacgcgg cagaccttcg
    33301 atatcgccaa actcaagttt gttaaagcga tatttaccac aggcgagggt caggatcagg
    33361 cagtcatccg gcacgctggt ggcgaaatcg gtgaagtagt ggcgctcgcc gcgtgcgccg
    33421 tcacagccac caagcaggaa gatatgacgc agtttttcac ggctaaccag atcaatcagc
    33481 gtatcggctg caccaagcag cgtctggcga ccaaagccca cggtgatcag gtgaggaatt
    33541 tcgctgtacg ggaagcccgc catttgttgc gcctgggcga tgaccgcaga gaaatcttcg
    33601 ccatccagat gacgcacgcc aggccagcca acgatgctgc gggtccagat acgatcgtca
    33661 taagcgccta cggttgggtc gatgatgcag ttcgaggtca tcacgatggg gccagggaaa
    33721 cgagcgaact ccacttgctg attctgccag ccgctgccgt agttaccgac cagatgcttg
    33781 aatttacgca gctccgggta gccatgcgca ggcagcattt cgccgtgggt gtagacatta
    33841 acgcccgtgc cttcggtctg ttccagcagg ttgtagagat ctttgagatc gtgaccggaa
    33901 atcagaatgc atttacccgc cgtcgctttg acgttgacct gggttggcgt cgggtgaccg
    33961 tatttaccgg tttcgcctgc atccagaatg ctcatcactt tgaagttcat ctggccgatt
    34021 tccattgaac actcaagaag cgcgttcata tcggcaggcc aggtccccag ccacgccatg
    34081 attttatggt actgggcata aatatcgttg tcgtattgac cgagaacatg cgcgtgttcc
    34141 atataggccg ccgcaccttt caggccatac aggcacagca gacgcaggcc gagaatgttt
    34201 tcgccaatcg ccgctttatc tttgttaggg gtaaattctg ctgcctgacg ttgcagctcg
    34261 ccgagatcat cgctcaccag ttgcaggtca gccatcgggt tatcgacgcg cgcgttggca
    34321 tctacagcca ggcattgtgc tttcagcgcc tcgcgcaagg caatcgcttc acgagcgtag
    34381 ccgacaatac gcggagaatc gaagttaacg ttggtcaggg ttgagaaaaa ggcacgtggc
    34441 gcgaagctgt caacatcgtg gttgatgatg ccgtattcac gcgcttttac cgcccaggca
    34501 gaaagccctt gcagcgccgc gatgagtaaa tcctgaaggt cagaagtttc cgccgtttta
    34561 ccacacatcc cctgcgcgta tgagcagccg ttttctgccg gagtacggat agtttgttca
    34621 cattgcacac aaaacatgat cacacctttt aaagttatat ttaatataca tgtttaaggg
    34681 taagacgctt aacgcgggga taaaagggat ttttcatgca actttaaggg agattgattt
    34741 agcgcaattt tggcggcagg gaactaccgc cagagaggta ttacgcagag aaaaaggcga
    34801 tgaggatcgg cactaacagg ctaagaataa aaccgtgaac aattgccgcc gggaccatat
    34861 ccagcccgcc agtacgttga agaacgggca gggtgaaatc cattgatgtg gcaccgcata
    34921 agcccagtgc agtagagcgg ctgcggcgaa tcagcccagg gatcaacata atagcaatca
    34981 gttcacgggc cagatcatta aaaaacgccg cgctcccgat taccggacca aaagattcgg
    35041 tcaataaaat accggaaaga gaataccagc cgaaaccgga ggccattgcc agcgcggtat
    35101 tgatggggag atcaagaata aaggcgttaa ttaaaccacc aattaatgaa ctgacaacca
    35161 ccaccacggc gacaatcatt ccccggcgat taaggacaat ctgctttaag gtcatgccat
    35221 tattgcgcaa ctgaatacca acgaggaaaa gtagcaaaat taacgtgtat tcactggctt
    35281 cggtcgcgtg ttgtaagaaa gccagtccac tcagaccaat ggcaaaacca atcactacta
    35341 cgccgcacag ttttagcgac tccagcgcca tcgcaatacg cgacgggagt ttttcttgct
    35401 gatggtggtt gcgccacggc aggcctcgct ccagccacat cagggcggca atattacaca
    35461 gtaaaataac ggtaatactg acggcagaat aatgcagaat cgccaacagg ttactggcga
    35521 ggttatcgag aaacgccaga ctgataccca taaaaaagag aataaggtaa accatccagc
    35581 ttaatagctg attaatagct tttaacgcag cttgttggcg aagcggaatg aggtaaccca
    35641 caatcagggg aaccagaatg attaacagcc cagaaaacat gaaaatccag tccttgcaaa
    35701 gaagaagtcg aaatgcgcga tgacacacta ctgaaagcgg aagggcgagt aaagttgcaa
    35761 t
//
LOCUS       DAJTRC010000033        34801 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.033, whole genome shotgun sequence.
ACCESSION   DAJTRC010000033 DAJTRC010000000
VERSION     DAJTRC010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.033"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..2099
                     /locus_tag="OO357_001751"
     CDS             <1..2099
                     /locus_tag="OO357_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="TcfC E-set like domain-containing protein"
                     /protein_id="HCR5886724.1"
                     /translation="FNGYDADDVSDSNTDVSDLYYRYDFLRRYYVQAGRMDNRTLFNA
                     QGGNFTFNFLPLGAIDGMRIGSTLSYLNQAQSQQGTPVMVLLSRNSRVDAYRNEQLLG
                     SFYLNSGSQFIDTSSFPPGSYSVALKVYENNQLTRTELVPFTKTGGLTDGNAQWFLQA
                     GKTTSQVSDDESSAYQLGVRLPLHPQYELYAGLANADDVSAFELGNNWTADLGRVGNL
                     AISASVFRNDDGGKGDMQQANWSNPGWPTLGFYRTNSDGDACTTDSRESYNALSCYES
                     ISATVSLNFVGWNMMLGYTCTQNNTDDSLRWDKQQSFENNYLRQTTAQSISETVQLSA
                     SRAIVMRDWILSTSVGVFHRNDNGGDNDDNGLYLSFSLSDTPTMDSNNNSHSTNVPTD
                     YRYSEQDGDQTSWQLSHTFYNDSFSHKELGVTVGGLNTDTINSAVNGRWDGQYGNVYA
                     TVSDSYDRKNHDHLSAFTGTYSSTLAVSRYGVNLGASGTDDLLGAVLVDVKGFSEQDE
                     ESQDLQLEARVAGSRTLQLGQSDSVLFPYPGFQSGFVEVNDSSQGNQQGTTNIINGAG
                     NRELMLLPGKLRYREVSASFNYNYIGRLLLPAAVKKFPIVGLNSAMLLVAEDGGFTLE
                     INGSEKELYLLSGQQFLKCPLSVVKKRASIRYSGDVTCSVVTYSQLPESIQVQAQLKQ
                     PKLRGNVQTAQREVAP"
     gene            2096..3184
                     /locus_tag="OO357_001752"
     CDS             2096..3184
                     /locus_tag="OO357_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein CblD"
                     /protein_id="HCR5886725.1"
                     /translation="MRNRLIAAILGLFGTLTGVQAAPDVTSEITYDLASGRADYYFWK
                     DEASAGNNGYMWYECSYPDLQQTCTANGNISTVQIYLTEQRSGMRWPVKLKGFKTAIV
                     SSDEAPLGCKGGKGLQTNLKDSNRSSCTEDGQHYYIYDTKFLTLYLEQTEMKNLPIGG
                     VWKGKVKLHSNSPAQDYFANITLNTLDPNHIDVFFPEFAHATPRVQLDLHPTGSVNGS
                     NYAQDLTMLDMCLYDGFNGNAISYEIMLKDEGRPAAGRRDGYFSIYRQGGTTTDEGER
                     IDYRVKMYNPETGGQIDVRNNENMVWNSINLKRVRPVVLPGIRYAVMCVPTPLTLAVD
                     KFSVMDKQAGYYMGKLSVIFTPSLPTIN"
     gene            complement(3272..4570)
                     /gene="exuT"
                     /locus_tag="OO357_001753"
     CDS             complement(3272..4570)
                     /gene="exuT"
                     /locus_tag="OO357_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexuronate transporter ExuT"
                     /protein_id="HCR5886726.1"
                     /translation="MRKIKGLRWYMIALVTLGTVLGYLTRNTVAAAAPTLMEELNIST
                     QQYSYIIAAYSAAYTVMQPVAGYVLDVLGTKIGYAMFAVLWAVFCGATALAGSWGGLA
                     VARGAVGAAEAAMIPAGLKASSEWFPAKERSIAVGYFNVGSSIGAMIAPPLVVWAIVM
                     HSWQMAFIISGALSFIWAMAWLIFYKHPRDQKHLTDEERDYIINGQEAQHQVSTAKKM
                     SVGQILRNRQFWGIALPRFLAEPAWGTFNAWIPLFMFKVYGFNLKEIAMFAWMPMLFA
                     DLGCILGGYLPPLFQRWFGVNLIVSRKMVVTLGAVLMIGPGMIGLFTNPYVAIMLLCI
                     GGFAHQALSGALITLSSDVFGRNEVATANGLTGMSAWLASTLFALVVGALADTIGFSP
                     LFAVLAVFDLLGALVIWTVLQNKPAIEVAQETHNDPAPQH"
     gene            5055..6467
                     /gene="uxaC"
                     /locus_tag="OO357_001754"
     CDS             5055..6467
                     /gene="uxaC"
                     /locus_tag="OO357_001754"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019077755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HCR5886727.1"
                     /translation="MTPFMTEDFLLDTEFARRLYHDYAKDQPIFDYHCHLPPQQIAED
                     YRFKNLYDIWLKGDHYKWRAMRTNGVAERLCTGDASDREKFDAWAATVPHTIGNPLYH
                     WTHLELRRPFGITGKLLSPSTADEIWNECNELLAQDNFSARGIMQQMNVKMVGTTDDP
                     IDSLEHHAEIAKDGSFTIKVLPSWRPDKAFNIEQATFNDYMAKLGEVSDTDIRRFADL
                     QTALTKRLDHFAAHGCKVSDHALDVVMFAEANEAELDSILARRLAGETLSEHEVAQFK
                     TAVLVFLGAEYARRGWVQQYHIGALRNNNLRQFKLLGPDVGFDSINDRPMAEELSKLL
                     SKQNEENLLPKTILYCLNPRDNEVLGTMIGNFQGEGMPGKMQFGSGWWFNDQKDGMER
                     QMTQLAQLGLLSRFVGMLTDSRSFLSYTRHEYFRRILCQMIGRWVEAGEAPADINLLG
                     EMVKNICFNNARDYFAIELN"
     gene            6482..7969
                     /gene="uxaA"
                     /locus_tag="OO357_001755"
     CDS             6482..7969
                     /gene="uxaA"
                     /locus_tag="OO357_001755"
                     /EC_number="4.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001199350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HCR5886728.1"
                     /translation="MQYIKIHALDNVAVALADLAEGTEVSVDNQTVTLRQDVARGHKF
                     ALTDIAKGANVIKYGLPIGYALADIAAGEHVHAHNTRTNLSDLDQYRYQLDFQDLPAQ
                     AADPEVQIYRRANGDVGVRNELWILPTVGCVNGIARQIQNRFLKETNNAEGTDGVFLF
                     SHTYGCSQLGDDHINTRTMLQNMVRHPNAGAVLVIGLGCENNQVAAFRETLGDIDPER
                     VHFMICQQQDDEIEAGIEHLHQLYNVMRNDKREPGKLSELKFGLECGGSDGLSGITAN
                     PMLGRFSDYVIANGGTTVLTEVPEMFGAEQLLMDHCRDEATFEKLVTMVNDFKQYFIA
                     HDQPIYENPSPGNKAGGITTLEDKSLGCTQKAGSSVVVDVLRYGERLKTPGLNLLSAP
                     GNDAVATSALAGAGCHMVLFSTGRGTPYGGFVPTVKIATNSELAAKKKHWIDFDAGQL
                     IHGKAMPQLLEEFIDTIVEFANGKQTCNERNDFRELAIFKSGVTL"
     gene            8052..8603
                     /locus_tag="OO357_001756"
     CDS             8052..8603
                     /locus_tag="OO357_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgjV family protein"
                     /protein_id="HCR5886729.1"
                     /translation="MTAYWLAQGVGVIAFLIGITTFFNRDERRFKKQLSVYSAVIGVH
                     FFLLGTYPAGASAILNAIRTLITLRTRSLWVMAIFIVLTGGIGLAKFHHPVELLPVIG
                     TIVSTWALFRCKGLTMRCVMWFSTCCWVIHNFWAGSIGGTMIEGSFLLMNGLNIIRFW
                     RMQKRGIDPFKVEKTPPAIDERG"
     gene            complement(8608..9852)
                     /gene="sstT"
                     /locus_tag="OO357_001757"
     CDS             complement(8608..9852)
                     /gene="sstT"
                     /locus_tag="OO357_001757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine transporter SstT"
                     /protein_id="HCR5886730.1"
                     /translation="MTTQRSPGLFRRLAHGSLVKQILVGLVLGILLAWISKPAAEAVG
                     LLGTLFVGALKAVAPILVLMLVMASIANHQHGQKTNIRPILFLYLLGTFSAALAAVVF
                     SFAFPSTLHLSSSAGDISPPSGIVEVMRGLVMSMVSNPIDALLKGNYIGILVWAIGLG
                     FALRHGNETTKNLVNDMSNAVTFMVKLVIRFAPFGIFGLVSSTLATTGFSTLWGYAQL
                     LVVLVGCMLLVALVVNPLLVWWKIRRNPFPLVLLCLRESGVYAFFTRSSAANIPVNMA
                     LCEKLNLDRDTYSVSIPLGATINMAGAAITITVLTLAAVNTLGIPVDLPTALLLSVVA
                     SLCACGASGVAGGSLLLIPLACNMFGISNDIAMQVVAVGFIIGVLQDSCETALNSSTD
                     VLFTAAACQAEDDRLANSALRN"
     gene            complement(10448..11413)
                     /gene="alx"
                     /locus_tag="OO357_001758"
     CDS             complement(10448..11413)
                     /gene="alx"
                     /locus_tag="OO357_001758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001098790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family membrane protein Alx"
                     /protein_id="HCR5886731.1"
                     /translation="MNTVGTPLLWGGFAVVVAIMLAIDLLLQGRRGAHAMTMKQAAAW
                     SLVWVTLSLLFNAAFWWYLVQTEGRAVADPQALAFLTGYLIEKSLAVDNVFVWLMLFS
                     YFSVPAALQRRVLVYGVLGAIVLRTIMIFTGSWLISQFDWILYIFGAFLLFTGVKMAL
                     AHEDESGIGDKPLVRWLRGHLRMTDTIDNEHFFVRKNGLLYATPLMLVLILVELSDVI
                     FAVDSIPAIFAVTTDPFIVLTSNLFAILGLRAMYFLLAGVAERFSMLKYGLAVILVFI
                     GIKMLIVDFYHIPIAVSLGVVFGILVMTFIINAWVNYRHDKQRGE"
     gene            complement(11684..12682)
                     /locus_tag="OO357_001759"
     CDS             complement(11684..12682)
                     /locus_tag="OO357_001759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005050984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5886732.1"
                     /translation="MIRFAVIGTNWITRQFVEAAHESGKYKLTAVYSRSLEQAQHFAN
                     DFSVEHLFTSLEAMAESDAIDAVYIASPNSLHFSQTQLFLSHKIHVICEKPLASNLAE
                     VDAAIACARENQVVLFEAFKTACLPNFHLLRQALPKAGKLRKVFFNYCQYSSRYQRYL
                     DGENPNTFNPAFSNGSIMDIGFYCLASAVALFGEPKSVQATASLLASGVDAHGVVVMD
                     YGDFSVTLQHSKVSDSVLGSEIQGEAGSLVIEKLSECQKVCFVPRGSQMQDLTQPQHI
                     NTMLYEAELFATLVDEHLVDHPGLAVSRITAKLLTEIRRQTGVIFPADSVKLYLPK"
     gene            complement(12761..13453)
                     /locus_tag="OO357_001760"
     CDS             complement(12761..13453)
                     /locus_tag="OO357_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vancomycin high temperature exclusion protein"
                     /protein_id="HCR5886733.1"
                     /translation="MLRAFARLLLRICFSRRTLKIACLLLLVAGATIFIADRVMVNAS
                     KQLTWGDVNAVPARNVGLLLGARPGNRYFTRRIDTAAALYHAGKVKWLLVSGDNGRKN
                     YDEASGMQQALIAKGVPAKVIFCDYAGFSTLDSVVRAKKVFGENHITIISQEFHNQRT
                     IWLAKQYGIDAIGFNAPDLNMKHGFYTQLREKLARVSAVIDAKILHRQPKYLGPSVMI
                     GPFSEHGCPAKE"
     gene            complement(13530..14033)
                     /locus_tag="OO357_001761"
     CDS             complement(13530..14033)
                     /locus_tag="OO357_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HCR5886734.1"
                     /translation="MSNLTYLQGYPEQLLSQVRTLINEQRLGDVLAKRYPGTHDYATD
                     KALWQYTQDLKNQFLRNAPPINKVMYDNKIHVLKNALGLHTAVSRVQGSKLKAKAEIR
                     VATVFRNAPEPFLRMIVVHELAHLKEKEHNKAFYQLCCHMEPQYHQLEFDTRLWLTQL
                     SLGQDKI"
     gene            14118..15254
                     /gene="rlmG"
                     /locus_tag="OO357_001762"
     CDS             14118..15254
                     /gene="rlmG"
                     /locus_tag="OO357_001762"
                     /EC_number="2.1.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(1835)-N(2))-methyltransferase
                     RlmG"
                     /protein_id="HCR5886735.1"
                     /translation="MSHLDNGFRSLTLQRFPATDDVNPLQAWEAADEYLLQQLDDTEI
                     LGPVLILNDAFGALSCALAEHKPYSIGDSYISELATRENLRLNGIDESSVKFLDSTAD
                     YPQQPGVVLIKVPKTLALLEQQLRALRKVVTSDTRIIAGAKARDIHTSTLELFEKVLG
                     PTTTTLAWKKARLINCTFNEPPLADAPQTVSWKLEGTDWTIHNHANVFSRTGLDIGAR
                     FFMQHLPENLEGEIVDLGCGNGVIGLTLLDKNPQAKVVFVDESPMAVASSRLNVETNM
                     PEALDRSEFMINNALSGVEPFRFNAVLCNPPFHQQHALTDNVAWEMFHYARRCLKING
                     ELYIVANRHLDYFHKLKKIFGNCTTIATNNKFVVLKAVKLGRRR"
     gene            15539..15853
                     /gene="higB"
                     /locus_tag="OO357_001763"
     CDS             15539..15853
                     /gene="higB"
                     /locus_tag="OO357_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin HigB"
                     /protein_id="HCR5886736.1"
                     /translation="MHLITQKALKDAAEKYPQHKTELVALGNTIAKGYFKKPESLKAV
                     FPSLDNFKYLDKHYVFNVGGNELRVVAMVFFESQKCYIREVMTHKEYDFFTAVHRTKG
                     KK"
     gene            15850..16266
                     /gene="higA"
                     /locus_tag="OO357_001764"
     CDS             15850..16266
                     /gene="higA"
                     /locus_tag="OO357_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin HigA"
                     /protein_id="HCR5886737.1"
                     /translation="MIAIADILQAGEKLTAVAPFLAGIQNEEQYTQALELVDHLLLND
                     PENPLLDLVCAKITAWEESAPEFAEFNAMAQAMPGGIAVIRTLMDQYGLTLSDLPEIG
                     SKSMVSRVLSGKRKLTLEHAKKLATRFGISPALFID"
     gene            complement(16311..18329)
                     /gene="fadH"
                     /locus_tag="OO357_001765"
     CDS             complement(16311..18329)
                     /gene="fadH"
                     /locus_tag="OO357_001765"
                     /EC_number="1.3.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 2,4-dienoyl-CoA reductase"
                     /protein_id="HCR5886738.1"
                     /translation="MSYPSLFAPLDLGFTTLKNRVLMGSMHTGLEEYPDGAERLAAFY
                     AERARHGVALIVSGGIAPDLTGVGMEGGAMLNDASQIPHHRTITEAVHQEGGKIALQI
                     LHTGRYSYQPHLVAPSALQAPINRFVPHELTHEEILQLIDDFARCAQLAREAGYDGVE
                     VMGSEGYLINEFLTLRTNQRSDQWGGDYRNRMRFAVEVVRTVRERVGNDFIIIYRLSM
                     LDLVEGGGTFAETVELAQAIEAAGATIINTGIGWHEARIPTIATPVPRGAFSWVTRKL
                     KGHVSLPLVTTNRINDPQVADDILSRGDADIVSMARPFLADAELLSKAQSGRADEINT
                     CIGCNQGCLDQIFVGKVTSCLVNPRACHETKMPILPAVQKKNLAVVGAGPAGLAFAIN
                     AAARGHQVTLFDAHSEIGGQFNIAKQIPGKEEFYETLRYYRRMIEVTGVTLKLNHTVT
                     ADQLQAFDETILASGIVPRVPPIDGIDHPKVLSYLDVLRDKAPVGNKVAIIGCGGIGF
                     DTAMYLSQPGESTSQNIARFCNEWGIDSSLQQAGGLSPQGMQIPRSPRQIVMLQRKAS
                     KPGQGLGKTTGWIHRTTLLSRGVKMIPGVSYQKIDDDGLHVVINGETQVLAVDNVVIC
                     AGQEPNRALAQPLIDSGKTVHLIGGCDVAMELDARRAIAQGTRLALEI"
     gene            complement(18555..20906)
                     /gene="ygjK"
                     /locus_tag="OO357_001766"
     CDS             complement(18555..20906)
                     /gene="ygjK"
                     /locus_tag="OO357_001766"
                     /EC_number="3.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HCR5886739.1"
                     /translation="MKIKTILTPVTCALLISFSAHAANADNYKNVINRTGAPQYMKDY
                     DYDDHQRFNPFFDLGAWHGHLLPDGPNTMGGFPGVALLTEEYINFMASNFDRLTVWQD
                     GKKVNFTLEAYSIPGALVQKLTAKDVQVEMTLRFATPRTSLLETKIISNKPLDLVWDG
                     ELLEKLEAKEGKPLSDKTIAGEYPDYQRKISATRDGLKVTFGKVRATWDLLTSGESEY
                     QVHKSLPVQTEINGNRFTSKAHINGSTTLYTTYSHLLTAQEVSKEQMQIRDILARPAF
                     YLTASQQRWEEYLKKGLTNPDATPEQTRVAVKAIETLNGNWRSPGGAVNYNTVTPSVT
                     GRWFSGNQTWPWDTWKQAFAMAHFNPDIAKENIRAVFSWQIQPGDSVRPQDVGFVPDL
                     IAWNLSPERGGDGGNWNERNTKPSLAAWSVMEVYNVTQDKAWLAEMYPKLVAYHDWWL
                     RNRDHNGNGVPEYGATRDKAHNTESGEMLFTVKKGDKEETQSGLNNYARVVEKGQYDS
                     LEIPAQVAASWESGRDDAAVFGFIDKEQLDKYVANGGKRSDWTVKFAENRSQDGTLLG
                     YSLLQESVDQASYMYSDNHYLAEMATILGKPEEAKRYRQLAQQLADYINTCMFDPTTQ
                     FYYDVRIEDKPLANGCAGKPIVERGKGPEGWSPLFNGAATQANADAVVKVMLDPKEFN
                     TFVPLGTAALTNPAFGADIYWRGRVWVDQFWFGLKGMERYGYRDDALKLADTFFRHAK
                     GLTADGPIQENYNPLTGAQQGAPNFSWSAAHLYMLYNDFFRKQ"
     gene            complement(20923..21342)
                     /locus_tag="OO357_001767"
     CDS             complement(20923..21342)
                     /locus_tag="OO357_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886740.1"
                     /translation="MNGYIQYDLAEGITWMNGLEITDGTGQLYLTGLFTPNFAARAWH
                     HTGRADGLDVPGSESGMMVSAMYEALKGVYLSTAYTYAKHRPDHADDETTSFMQFGIW
                     YEYGGGRFATAFDSRFYMKNASNDPSDQIFLMQYFYW"
     gene            complement(21320..21673)
                     /locus_tag="OO357_001768"
     CDS             complement(21320..21673)
                     /locus_tag="OO357_001768"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886741.1"
                     /translation="MPDHSWGGFEGGINRFYGGYRTNDGTEIMLSLRQDSSLDDLQWW
                     GDFTPDLGYVIPNTRDIMTALKVQNLSGNFRYSVTATPAGHHDESKAWLHFGKYDRYD
                     DKYTYPAMDERLHPV"
     gene            complement(21674..21994)
                     /locus_tag="OO357_001769"
     CDS             complement(21674..21994)
                     /locus_tag="OO357_001769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886742.1"
                     /translation="MKLITAPCRALLALPFCYAFSAAGEEARPAEHDDTKTPAITSTS
                     SPSFRFYGELGLGGYMDLEGENKHKYSDGTYIEGGLEMKYGSWFGLIYGEGWTVQADH
                     DGNA"
     gene            complement(22128..23561)
                     /locus_tag="OO357_001770"
     CDS             complement(22128..23561)
                     /locus_tag="OO357_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5886743.1"
                     /translation="MSDTKRNTIGKFGLLSLTFAAVYSFNNVINNNIELGLASAPMFF
                     LATIFYFIPFCLIIAEFVSLNKNSEAGVYAWVKSSLGGRWAFITAYTYWFVNLFFFTS
                     LLPRVIAYASYAFLGYEYIMTPVATTVISMVLFAFSTWVSTNGAKMLGPITSVTSTLM
                     LLLTLSYILLAGTALVGGVQPADPITVDAMIPNFNWAFLGVTTWIFMAAGGAESVAVY
                     VNDVKGGSKSFVKVIILAGIFIGVLYSVSSVLINVFVSSKELKFTGGSVQVFHGMAAY
                     FGLPEALMNRFVGLVSFTAMFGSLLMWTATPVKIFFSEIPEGIFGKKTVELNENGVPA
                     RAAWIQFLIVIPLMIIPMLGSNTVQDLMNTIINMTAAASMLPPLFIMLAYLNLRAKLD
                     HLPRDFRMGSRRTGIIVVSMLIAIFAVGFVASTFPTGANILTIIFYNVGGIVIFLGFA
                     WWKYSKYIKGLTAEERHIEATPASNVD"
     gene            complement(23624..24073)
                     /locus_tag="OO357_001771"
     CDS             complement(23624..24073)
                     /locus_tag="OO357_001771"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit beta"
                     /protein_id="HCR5886744.1"
                     /translation="MRIIDNLEQFRQIYASGKKWQRCVEAIENIDNIQPGVAHSIGDS
                     LTYRVENDSATDALFTGHRRYFEVHYYLQGQQKIEYAPKETLQVVEYYRDETDREYLK
                     GCGETVEVHEGQIVICDIHEAYRFICNNAVKKVVLKVTIEDGYFHNK"
     gene            complement(24070..27156)
                     /gene="ebgA"
                     /locus_tag="OO357_001772"
     CDS             complement(24070..27156)
                     /gene="ebgA"
                     /locus_tag="OO357_001772"
                     /EC_number="3.2.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase subunit alpha"
                     /protein_id="HCR5886745.1"
                     /translation="MNRWENIQLTHENRLAPRAYFFSYDSVAQARTFARETSSLFLPL
                     SSQWNFHFFDHPLQVPEAFTSELMADWGHITVPAMWQMEGHGKLQYTDEGFPFPIDVP
                     FVPSDNPTGAYQRIFTLSDGWQGKQTLIKFDGVETYFEVYVNGQYVGFSKGSRLTAEF
                     DISAMVKTGDNLLCVRVMQWADSTYVEDQDMWWSAGIFRDVYLVGKHLTHINDFTVRT
                     DFDEAYCDATLSCEVVLENLAASPVVTTLEYTLFDGERVVHSSAIDHLAIEKLTSASF
                     AFTVEQPQQWSAESPYLYHLVMTLKDANGNVLEVVPQRVGFRDIKVRDGLFWINNRYV
                     MLHGVNRHDNDHRKGRAVGMDRVEKDLQLMKQHNINSVRTAHYPNDPRFYELCDIYGL
                     FVMAETDVESHGFANVGDISRITDDPQWEKVYVERIVRHIHAQKNHPSIIIWSLGNES
                     GYGCNIRAMYHAAKALDDTRLVHYEEDRDAEVVDIISTMYTRVPLMNEFGEYPHPKPR
                     IICEYAHAMGNGPGGLTEYQNVFYKHDCIQGHYVWEWCDHGIQAQDDHGNVWYKFGGD
                     YGDYPNNYNFCLDGLIYSDQTPGSGLKEYKQVIAPVKIHALTRGELKVENKLWFTTLD
                     DYTLHAEVRAEGETLATQQIKLRDVAPNSEAPLQITLPQLDAREAFLNITVTKDSRTR
                     YSEAGHSIATYQFPLKENTAQPVPFAPNNARPLTLEDDRLSCTVRGYNFAITFSKMSG
                     KPTSWQVNGESLLTREPKINFFKPMIDNHKQEYEGLWQPNHLQIMQEHLRDFAVEQSD
                     GEVLIISRTVIAPPVFDFGMRCTYIWRIAADGQVNVALSGERYGDYPHIIPCIGFTMG
                     INGEYDQVAYYGRGPGENYADSQQANIIDIWRSTVDAMFENYPFPQNNGNRQHVRWTA
                     LTNRHGNGLLVVPQRPINFSAWHYTQENIHAAQHCNELQRSDDITLNLDHQLLGLGSN
                     SWGSEVLDSWRVWFRDFSYGFTLLPVSGGEATAQSLASYEFGAGFFSTNLHSENKQ"
     gene            complement(27339..28322)
                     /gene="ebgR"
                     /locus_tag="OO357_001773"
     CDS             complement(27339..28322)
                     /gene="ebgR"
                     /locus_tag="OO357_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708881.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator EbgR"
                     /protein_id="HCR5886746.1"
                     /translation="MATLKDIAIEAGVSLATVSRVLNDDPTLNVKEETKHRILEIAEK
                     LEYKTSSARKLQTGAVNQHHILAIYSYQQELEINDPYYLAIRHGIETQCEKLAIELTN
                     CYEHNGLPDIKNVTGILIVGKPTPALRAAASALTDNICFIDFHEPGSGYDAVDIDLAR
                     ISKEIIDFYINQGVNRIGFIGGEDEPGKADIREVAFAEYGRLKQVVREEDIWRGGFSS
                     SSGYELAKQMLAREDYPKALFVASDSIAIGVLRAIHERGLNIPQDISLISVNDIPTAR
                     FTFPPLSTVRIHSEMMGSQGVNLVYEKARDGRALPLLVFVPSKLKLRGTTR"
     gene            28540..28871
                     /locus_tag="OO357_001774"
                     /pseudo
     CDS             28540..28871
                     /locus_tag="OO357_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417545.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-binding protein"
     gene            complement(28913..30292)
                     /gene="ygjG"
                     /locus_tag="OO357_001775"
     CDS             complement(28913..30292)
                     /gene="ygjG"
                     /locus_tag="OO357_001775"
                     /EC_number="2.6.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417544.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine aminotransferase"
                     /protein_id="HCR5886747.1"
                     /translation="MNRLPSSASALACSAHALNLIEKRTLDHEEMKALNREVIEYFKE
                     HVNPGFLEYRKSVTAGGDYGAVEWQAGGLNTLVDTQGQEFIDCLGGFGIFNVGHRNPV
                     VVSAVQNQLAKQPLHSQELLDPLRAMLAKTLAALTPGKLKYSFFCNSGTESVEAALKL
                     AKAYQSPRGKFTFIATSGVFHGKSLGALSATAKSTFRKPFMPLLPGFRHVPFGNIEAM
                     RTALNECKKTGDDVAAVILEPIQGEGGVILPPPGYLTAVRKLCDEFGALMILDEVQTG
                     MGRTGKMFACEHENVQPDILCLAKALGGGVMPIGATIATEEVFSVLFDNPFLHTTTFG
                     GNPLACAAALATINVLLEQNLPAQAEQKGDMLLDGFRQLAREYPDLVQEARGKGMLMA
                     IEFVDNEIGYNFASEMFRQRVLVAGTLNNAKTIRIEPPLTLTIEQCELVIKAARKALA
                     AMRVSVEEA"
     gene            30710..32230
                     /gene="aer"
                     /locus_tag="OO357_001776"
                     /pseudo
     CDS             30710..32230
                     /gene="aer"
                     /locus_tag="OO357_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311981.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="aerotaxis sensor receptor Aer"
     gene            complement(32384..33007)
                     /gene="nfeR"
                     /locus_tag="OO357_001777"
                     /gene_synonym="yqjI"
     CDS             complement(32384..33007)
                     /gene="nfeR"
                     /locus_tag="OO357_001777"
                     /gene_synonym="yqjI"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NfeR"
                     /protein_id="HCR5886748.1"
                     /translation="MSHHHEGCCKHEGQPRHEGCCKGEKSEHEHCGHGHQHEHGQCCG
                     GRHGRGGGRRQRFFGHGELRLVILDILSRDDSHGYELIKAIENLTQGNYTPSPGVIYP
                     TLDFLQEQSLITIREEEGGKKQIALTEQGAQWLEENREQVEMIEERIKARCVGAALRQ
                     NPQMKRALDNFKAVLDLRVNQSDISDAQIKKIIAVIDRAAFDITQLD"
     gene            33284..34029
                     /gene="nfeF"
                     /locus_tag="OO357_001778"
                     /pseudo
     CDS             33284..34029
                     /gene="nfeF"
                     /locus_tag="OO357_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417541.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent ferric chelate reductase"
     gene            complement(34083..34158)
                     /locus_tag="OO357_001779"
     tRNA            complement(34083..34158)
                     /locus_tag="OO357_001779"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(34123..34125),aa:Ile,seq:cat)
     gene            34283..34789
                     /gene="mug"
                     /locus_tag="OO357_001780"
                     /pseudo
     CDS             34283..34789
                     /gene="mug"
                     /locus_tag="OO357_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417540.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="G/U mismatch-specific DNA glycosylase"
BASE COUNT         8490 a         8949 c         9143 g         8219 t
ORIGIN      
        1 atttcaacgg ttatgatgca gatgatgtca gtgacagtaa tactgacgtc agcgatctct
       61 attatcgtta tgatttttta cgtcgttatt atgtgcaggc gggtcgcatg gacaaccgca
      121 cactatttaa tgcacaaggc gggaacttta cctttaactt tctgccactc ggtgcaatcg
      181 atgggatgcg tatcgggtcg acactgagct atttaaacca ggcgcaaagc cagcaaggaa
      241 ccccagtaat ggttctgctt tcgcgcaatt ctcgtgttga cgcttatcgt aatgagcaac
      301 ttttgggatc gttttatctc aatagtggtt cgcaatttat tgataccagt tcctttccgc
      361 caggcagcta cagcgtggca ttaaaagtct acgaaaataa ccaactcacc cgcaccgagc
      421 ttgtgccgtt taccaaaacc ggcggtctga ctgacggaaa tgcgcaatgg ttcttacagg
      481 caggtaaaac tacatcacag gtttctgatg atgaaagttc agcttatcag ctaggagtac
      541 gcctgccatt acatccgcaa tatgagctct acgcagggct ggcgaatgct gatgatgtga
      601 gtgctttcga gttaggtaat aactggacgg cagatttagg cagggtgggg aatcttgcaa
      661 tcagcgccag cgtgttccgt aacgatgacg gcggcaaagg tgatatgcaa caggccaact
      721 ggagtaatcc gggatggccg acgttgggct tttatcggac caactctgac ggcgatgctt
      781 gtacaaccga cagcagagag agctataacg ccttaagctg ttatgaaagt atttccgcga
      841 cagtttcact gaattttgtc ggctggaata tgatgctggg ttatacctgc acacaaaata
      901 acactgatga tagtttgcgt tgggataaac agcagagctt tgaaaataac tatcttcgcc
      961 agacaacagc gcaaagtatc tctgaaactg tacaacttag cgcttcccgc gctattgtga
     1021 tgcgtgactg gattttgagt acgtcagtcg gtgttttcca tcgtaatgac aacggtggcg
     1081 ataacgacga caacggcttg tacttatcgt tttcgttatc tgacacgcca acgatggata
     1141 gcaataacaa cagccattca accaatgtgc ctacggatta tcgttatagc gaacaggatg
     1201 gcgatcaaac gtcatggcag ttatcgcata ccttttataa cgattcattc agccataaag
     1261 aacttggcgt aaccgtcgga ggcctgaaca ccgataccat aaacagcgcg gttaacgggc
     1321 gttgggatgg gcaatacgga aatgtctacg ctaccgtatc tgatagttat gaccgtaaga
     1381 atcatgatca tctttcggcc tttacgggca cttatagctc tacgctggct gtcagtcgct
     1441 atggcgttaa tttgggagcc agtggtacag acgatttgct gggtgcggta ctggtggatg
     1501 tgaaaggctt ctctgaacag gatgaagaga gtcaggatct gcaactcgaa gcgcgggtgg
     1561 caggcagccg aactttgcag cttggtcaaa gtgacagtgt gttgttccct tatcctggat
     1621 ttcagtctgg ttttgttgag gttaacgaca gtagccaggg caatcagcag gggacaacaa
     1681 acatcattaa cggtgcaggg aatcgtgaat taatgttgtt gcctggcaag ctgcgctatc
     1741 gcgaagtgtc tgccagcttt aactacaact atatcggtcg tttattactg ccggcagcgg
     1801 tgaaaaaatt cccgattgtg gggctgaaca gcgccatgtt actggtagct gaagatggcg
     1861 gatttacact tgaaattaac ggcagcgaaa aagagctcta tctgctttcc gggcagcaat
     1921 tccttaagtg tccactgagt gttgtaaaga aacgcgccag cattcgttac agcggagatg
     1981 ttacttgtag tgtggtgact tattcacaat taccggagtc gattcaggtt caggcacagt
     2041 tgaaacagcc taaattacgt ggaaacgttc agacggcgca aagggaggtt gcaccatgag
     2101 aaatcgattg attgcggcga tattgggttt gtttggcacg ctcactggcg ttcaggcagc
     2161 tcctgacgtg accagtgaaa ttacgtatga tttggcatct ggtagagcgg attattactt
     2221 ctggaaggat gaagcgtcgg caggaaataa tggatatatg tggtatgaat gttcgtatcc
     2281 tgacctccaa caaacctgta cagctaatgg aaatatatcg acagtacaaa tctatttaac
     2341 tgaacaacgc agtgggatgc gttggccggt aaaactcaaa ggatttaaaa cagccattgt
     2401 aagtagcgat gaagcgccgc taggatgcaa ggggggcaaa gggcttcaga cgaatcttaa
     2461 ggattctaat agatcttcat gtacagaaga tggtcaacat tattatatat acgatacaaa
     2521 gtttcttacg ctctaccttg agcagacaga gatgaagaat ttgccgattg gtggcgtctg
     2581 gaaggggaaa gttaaattac attcgaacag cccggcccag gactatttcg caaatattac
     2641 cctgaatacg ctcgacccca accatattga cgtgttcttc ccggagttcg cccacgccac
     2701 gccaagggtg cagttagact tgcatccaac aggaagcgtt aacggcagca actacgcgca
     2761 agatctgacc atgttggaca tgtgcctgta cgatggtttt aacggtaatg ccatcagtta
     2821 tgaaatcatg ctcaaagatg aagggcgacc agctgcaggg cgcagagacg gttacttctc
     2881 tatctatcgt cagggaggga ccaccaccga cgagggagaa cgcattgatt accgggtcaa
     2941 aatgtacaac ccggaaaccg gtgggcaaat tgatgtgcgc aataatgaaa atatggtctg
     3001 gaacagcatt aacctgaaac gtgtgcgtcc ggtggtactg cccggtattc gctatgccgt
     3061 gatgtgtgtg ccgacaccct tgactctggc agtcgataaa ttcagcgtga tggataaaca
     3121 ggccgggtac tacatgggca aattgtcagt aatctttacg ccttccttgc caaccatcaa
     3181 ttaaccctgc aaaccggaca aacgcttttt tctatataaa cgacgaagcc gcctgataag
     3241 gcggcttcgt cgttataact tacgttaaac gttaatgttg cggtgcggga tcgttatgtg
     3301 tttcctgcgc cacctcaatc gccggtttgt tctgcaatac ggtccagata accagcgcac
     3361 ccagcaggtc aaatactgcc agaactgcga acagcgggct gaagccgatg gtgtcagcca
     3421 gtgcaccgac aaccagcgca aacagcgtac ttgccagcca tgcggacatc ccggttaaac
     3481 cgttcgccgt cgccacttcg ttacgaccga acacgtcaga agagagcgta atcagcgcgc
     3541 cagacagtgc ctggtgggca aaaccaccga tacacagcag cataattgcg acatacgggt
     3601 tggtgaacag accgatcata cccgggccaa tcatcagcac agcacccagc gttacgacca
     3661 tcttacggga aacgatcagg ttcacaccaa accaacgctg gaacagcggc ggcaggtaac
     3721 caccgaggat acaaccgagg tcagcaaaca gcatcggcat ccaggcgaac atcgcgatct
     3781 ctttcaggtt aaagccgtaa actttaaaca tgaacagcgg gatccacgcg ttaaaagtac
     3841 cccaggccgg ttctgcaagg aaacgcggca gtgcgatacc ccagaactga cggttacgca
     3901 ggatctgacc aacggacatt ttcttcgccg tgctcacctg gtgctgggct tcctgaccat
     3961 taataatata gtcgcgttct tcatcggtca gatgcttctg gtcgcgcgga tgtttataga
     4021 aaatcagcca tgccatcgcc cagataaagc tcaatgcacc ggagatgata aatgccatct
     4081 gccagctgtg catcacgatt gcccatacca ccagcggcgg cgcaatcatc gcaccaatcg
     4141 aagaacctac gttaaagtag cctactgcga tggaacgctc tttcgccggg aaccattcgg
     4201 agctggcttt cagacccgcc gggatcatcg ctgcttccgc ggcaccgacc gcaccacgag
     4261 caacagctag gccaccccag ctacctgcca gcgcggttgc accacagaac acggcccaca
     4321 gcacagcaaa cattgcataa ccgattttcg tacccagcac atccagtaca taacctgcta
     4381 ccggttgcat gaccgtataa gcagcagaat aggctgcgat gatataggaa tactgttggg
     4441 tggagatgtt taactcttcc atcagagttg gcgcagctgc cgccacagtg ttacgcgtca
     4501 ggtaaccaag cacggtgccg agcgtcacca gtgcgatcat ataccaacgt aaccctttaa
     4561 ttttacgcat gtaaaacctc atcgttttgt tatatccgca ggaagcgcgg ccatcatccc
     4621 gccagaggct gaagatgtta gttacgaccg ggtaaccgaa aaaaaaaacc ggcacgcccc
     4681 gaacgttgcc atgaaagttt cgtgctaatt cggcttccgt accggttaac ccgattcatc
     4741 taacaaatga atgaatcggc aatttgcatt ccgtcgactt atgatttgcg acggcagaaa
     4801 gataacttgt catacaacct taaaaggtga gagccatcac aaatgtgcga atatttgtag
     4861 ggacattacc tgacgacagc gaggccagta ctggcgcggc ctgcagcgag atttaccact
     4921 ttgagagtaa tttttttaac tacgtttatt gatctaactc acgaaaatat cttcggactc
     4981 tggaaattgg tgtgataact ttgtcagcat cgcaccataa gcaagctagc tcactcgttg
     5041 agaggaagac gaaaatgact ccgtttatga ctgaagattt cctgttagat accgaatttg
     5101 cccgccgtct gtatcacgac tacgcaaaag accagccgat tttcgattac cattgccatt
     5161 tgccgccgca gcagattgcg gaagactatc gttttaaaaa cctgtatgac atctggttga
     5221 agggcgatca ctacaaatgg cgcgctatgc gtaccaacgg tgtggctgag cgtctgtgta
     5281 ctggtgatgc gtctgaccgt gaaaaatttg acgcctgggc ggcgactgtt ccgcacacta
     5341 tcggcaaccc gttataccac tggacgcacc tcgaactgcg tcgtccgttc ggtatcactg
     5401 gcaaattgct ttctccgtca actgccgatg aaatctggaa cgaatgtaac gaactgctgg
     5461 cgcaggataa cttctccgcg cgcggcatca tgcagcagat gaacgtgaaa atggtcggca
     5521 ccaccgatga cccgatcgat tctctggagc atcacgcaga gatcgccaaa gacggctctt
     5581 tcaccatcaa agtgctgccg agctggcgtc cggacaaagc cttcaatatc gaacaggcga
     5641 cctttaacga ctacatggcg aagctgggcg aagtttccga taccgacatt cgccgctttg
     5701 ctgacctgca aactgccttg accaaacgtc tggatcactt cgccgctcac ggctgtaaag
     5761 tgtctgacca tgcgctggac gtggtgatgt ttgctgaagc gaacgaagcg gaactggaca
     5821 gcattctggc gcgccgtctg gctggcgaaa ccctgagcga gcacgaagtg gcacagttca
     5881 aaactgcggt actggtgttc cttggtgccg aatatgcacg tcgcggctgg gtacagcagt
     5941 accacattgg cgcgctgcgt aataacaacc tgcgtcagtt caaactgctg gggccggatg
     6001 taggctttga ctccatcaac gaccgtccga tggcggaaga gttgtctaag ctgctgagca
     6061 agcagaacga agaaaacctg ctgccgaaaa ccattctcta ctgcctgaac ccgcgcgata
     6121 acgaagtgct gggcaccatg atcggtaact tccagggcga aggtatgccg ggcaaaatgc
     6181 agttcggttc cggctggtgg tttaacgatc agaaagacgg tatggaacgt cagatgaccc
     6241 aactggcgca gctcggtctg ctgagccgct ttgtcggtat gctgactgac agccgtagct
     6301 tcctgtcata cacccgtcac gaatacttcc gccgcattct gtgccagatg atcggtcgct
     6361 gggtggaagc gggcgaagca ccggcggaca tcaacctgct gggcgagatg gtgaaaaata
     6421 tttgctttaa caatgcgcgt gactacttcg ccattgaact gaactaaggt ctggggttga
     6481 tatgcaatac atcaagatcc atgcgctgga taacgtcgcg gtcgctttag cagatttggc
     6541 tgaaggcaca gaagtcagtg tcgataacca gactgttacg ctgcgccagg atgttgctcg
     6601 tggacataaa tttgcgttaa cggatatcgc aaaaggggcc aacgtcatca aatatggcct
     6661 gccgattggt tatgcattgg cggatattgc ggcgggggaa cacgtccacg cccacaatac
     6721 gcgcacgaat ctgagcgatc tggatcagta tcgctatcaa cttgattttc aggatctgcc
     6781 tgcgcaagcg gcagatcctg aagtgcagat ctatcgtcgc gctaacggcg atgtcggggt
     6841 gcgtaacgag ctgtggatcc tgccaaccgt gggctgtgtc aacggcatcg cgcggcaaat
     6901 ccagaaccgt ttcctgaaag agaccaacaa cgccgaaggt accgacggcg tgttcctctt
     6961 cagccacacc tatggctgct cacaactggg cgacgatcac atcaacaccc gcaccatgct
     7021 gcaaaacatg gtgcgccacc cgaacgcggg cgcagtgctg gtgattggtc tgggctgtga
     7081 aaacaaccag gttgccgcat tccgtgaaac gttgggcgat atcgatcctg aacgcgttca
     7141 tttcatgatc tgccaacagc aggatgatga gatcgaagcc gggatcgagc atttgcatca
     7201 gctgtataac gtgatgcgca acgataaacg cgagccaggc aaactcagcg aactgaagtt
     7261 tggtctggag tgcggtggtt ctgacggtct ttctggtatt actgctaacc cgatgctggg
     7321 gcgtttctct gactacgtga ttgctaacgg cggtactacc gtactgaccg aagtgccgga
     7381 gatgtttggc gcagagcagt tgctgatgga ccattgccgc gacgaagcaa cgtttgaaaa
     7441 actggtcacc atggtcaacg acttcaaaca gtactttatt gcccatgatc agccgatcta
     7501 cgaaaaccca tcaccgggga acaaagcggg cggtatcacc acgctggaag acaaatcact
     7561 tggctgtacc cagaaagcgg gttccagtgt cgtggttgac gtgctgcgtt acggcgagcg
     7621 tctgaaaacg ccagggctga acttgttaag tgcgccgggt aacgatgccg tagcgaccag
     7681 cgccctggcg ggtgcgggct gccatatggt gctgttcagt actggtcgcg gcacgccata
     7741 cggtggattt gtgccgacgg tgaaaatcgc caccaacagt gaactggcgg cgaagaaaaa
     7801 acactggatc gactttgacg cgggtcagct gatccacggt aaagcgatgc cgcagttgct
     7861 ggaagaattt atcgacacca tcgttgagtt tgccaacggt aagcaaacct gcaacgagcg
     7921 taacgacttc cgcgaactgg cgatcttcaa aagcggcgta acgctataaa gtcgaaatga
     7981 gttgttaata aaaaacggcg tttcataatg tgaagcgccg tttgcttttc catacctgct
     8041 aaggatttat catgaccgcg tattggctgg cccagggcgt gggtgtcatc gcctttctga
     8101 ttggtatcac aacatttttc aatcgtgacg aacgtcgctt caaaaagcag ctttcggtct
     8161 atagcgccgt tattggcgta catttttttc ttctgggcac ctatcccgct ggtgccagtg
     8221 ccatccttaa tgccattcgt acattgatta ccttacgcac gcgcagctta tgggtaatgg
     8281 cgatttttat tgtgctgact ggcggaattg gcctcgcgaa gttccatcat cctgtcgaac
     8341 tattgccggt tatcggtacg attgtcagta cctgggcgct gttccgctgt aaagggctca
     8401 ccatgcgctg cgtgatgtgg ttttcaacgt gttgctgggt gattcacaac ttctgggcgg
     8461 ggtcgatagg cggcacgatg attgagggta gttttctgct tatgaatggc ctgaatatca
     8521 ttcgtttctg gcggatgcag aaaaggggaa ttgatccgtt taaagtagag aaaacccctc
     8581 ccgccataga cgaaaggggt taaacaatta attacgcagg gcgctatttg ccagacgatc
     8641 gtcttccgcc tggcaagctg ccgcagtgaa cagcacgtca gttgaagagt tcagcgcggt
     8701 ttcgcaagag tcctgcaata cgccgataat aaagccgacg gcaaccacct gcatggcgat
     8761 atcgttcgaa ataccgaaca tattacaggc cagtgggatc agcagcaaag aaccccccgc
     8821 cacgccggat gcgccacagg cacacagaga agccactacg ctcaacagca gcgctgtggg
     8881 cagatcgacc ggaataccca gcgtattaac cgcagccagc gtcaacacgg taatggtgat
     8941 tgctgcgccc gccatattga tggtggctcc cagcggaata gaaactgaat aggtatcgcg
     9001 atccagattc agcttttcac acagcgccat attcaccgga atgttcgctg cagagctgcg
     9061 ggtgaagaag gcatacacac cgctttcgcg caggcacagc agcaccagcg ggaacgggtt
     9121 acgacgaatt ttccaccaca ccagcaatgg gttaaccacc agcgccacca gtaacataca
     9181 gccaaccagc acgaccagca gttgcgcgta gccccacagt gtggagaaac cggtggttgc
     9241 cagggtagaa gaaaccagcc caaaaatacc gaacggtgcg aagcgaatga ccagtttcac
     9301 cataaaggta acggcattcg acatatcatt gaccaggttt ttggtggtct cgttaccgtg
     9361 acgcagtgcg aagccgaggc cgatcgccca caccagaatc ccgatgtagt tacctttcag
     9421 cagggcgtcg atggggttgg aaaccatgct cattaccagt ccgcgcatca cttcgacaat
     9481 gcctgacggc ggcgaaatat cacccgcgct actggacaag tgcagggtag aagggaaggc
     9541 aaagctgaag actactgcgg ccagagctgc agagaaggtg cccagcagat agaggaacaa
     9601 aataggacgg atattggttt tctgcccgtg ctggtggtta gcaatagatg ccattaccag
     9661 catcaacacc aggatggggg caacagcttt cagtgcgccg acgaacaaag tacctaacag
     9721 accaacagct tccgccgcgg gttttgagat ccatgccaga agaatcccca gaacaaggcc
     9781 gaccaggatt tgttttacca ggctgccatg agccagacgc cggaatagcc ccggtgaacg
     9841 ttgcgtagtc atttttcgat cctttcattg tgttgtcgca catccctggt gcgttctgtc
     9901 gacgcatctt tcaggatgta acaaagtgtt tgcaaggtcg agtataagga aagttacagc
     9961 ttcgggaagc gaaaaatgct ggattttaaa gagaggtcat gttttgcaac ttaatggttg
    10021 catttattta acataaacag agggaaggac tggctactaa ggctgattag ataacatatg
    10081 ggcaaaacta tttggaatat gagaacggtt gcgctccttt aacgtgttat gccatataaa
    10141 gtcactggcg cagggagcgc gcagggggcg gccaatcgcc gccgccccct gcacccccag
    10201 gctctggcga acaaaatcgc cgctgcgcgg ttccctcagc ctatcccttg cggctaccgg
    10261 gtcgggcgca aggtaacgtc cctgtaagat gcgccctcgc cccggcctcc agggaacgct
    10321 tcggcgattt tgacgccacc aacacccgtg cggtcttttt gtaaagagct ataagccgat
    10381 tacataatta atagctcacc tttgtctgga atgagttatc tctaggaatg gcttaatata
    10441 cagcgaatca ttctccccgc tgcttatcat gccgataatt cacccaggcg ttgataataa
    10501 acgtcatcac cagaatgcca aacaccacgc ccagcgagac ggcgattgga atatggtaga
    10561 agtcgacaat cagcatcttg ataccgataa acaccagaat caccgccagg ccatatttga
    10621 gcatcgagaa acgctctgct acgcccgcca gcaggaaata catcgcacgc aggccgagga
    10681 ttgcaaacag gtttgaggtc agcacaatga acgggtcagt ggtcacggcg aagatagccg
    10741 gaatgctatc cacggcaaaa atcacgtcgc tcaattccac cagaatcagt accagcatca
    10801 gcggtgtggc gtacagcaat ccattcttac gcacaaagaa atgctcgttg tcgatggtgt
    10861 cggtcatgcg caaatgaccg cgtagccagc gcaccagcgg cttgtcgcca atgcctgatt
    10921 catcttcatg ggcgagcgcc atcttcacgc cggtaaacag caggaaggca ccgaagatat
    10981 acaggatcca gtcgaactgc gaaatcagcc agctgcctgt gaagatcatg atggtacgca
    11041 gaacaatcgc cccgagcacg ccatacacca gcacgcgacg ttgtaatgcc gccggaacgg
    11101 agaaatagct gaacaacatc agccagacaa agacgttatc gaccgccagc gatttctcaa
    11161 tcagataacc cgtgagaaag gccagggcct gtggatcggc aacggcgcga ccttcggttt
    11221 gcaccagata ccaccagaaa gcggcgttaa acagtaacga cagcgtcacc cagaccagcg
    11281 accaggccgc agcctgtttc atggtcatgg catgtgcccc acgacgcccc tgcaacaaca
    11341 ggtcgatagc cagcataatg gcgacaacaa cagcgaatcc gccccatagc aacggcgtgc
    11401 cgacagtatt catagaagtt ccttacacat aaaaaaacgg ccaacgtcgg aagacgtcag
    11461 ccgctgcttt ttatgcatag acctcgcctt ccggcaaggt ctcacttaca acaaaaaagg
    11521 aatacgtctg cgtattcctt tcgggttgcc cggtgaccgg atgtggtttt tcactcatcg
    11581 taatgacgat cgaccggcaa tgaagttact cccctttgca ggtaacaaag tatgacagac
    11641 cattcagtca gtcaatggtc tgttacatac cttttacact gttttacttt ggcaagtata
    11701 gttttacgct atctgccgga aaaatcaccc cagtttggcg gcggatctcg gtcagcagtt
    11761 tggctgtgat gcgactgacc gccagccctg gatgatccac cagatgctca tccaccaggg
    11821 tagcgaacag ctctgcttcg tagagcatgg tattaatatg ctgcggctgg gtgagatcct
    11881 gcatctggct gccacgcggc acaaagcaga ctttctggca ttcagacagt ttttcaatca
    11941 ccagcgatcc cgcttcgccc tgaatctcgc ttcccaggac agaatcactg acctttgagt
    12001 gctgcaaggt gacgctgaaa tcaccgtaat ccatcaccac cacgccgtgg gcgtcaacgc
    12061 cgcttgccag caaactcgcc gttgcctgca cgcttttcgg ctcaccaaat aacgccaccg
    12121 ccgacgccag gcagtaaaag ccgatatcca taattgaacc gttagagaat gccggattaa
    12181 aggtgttggg attctcaccg tccaggtaac gttgataccg cgaggaatac tggcaatagt
    12241 tgaaaaagac tttacgcagt ttgcctgctt tcggcagcgc ctggcgcaac aaatgaaagt
    12301 tcggcaggca ggcggtttta aatgcctcaa acagcaccac ctgattttcg cgcgcacagg
    12361 caatggcggc atccacttcc gccagattcg acgccagcgg tttttcgcaa atcacatgaa
    12421 ttttatggct aaggaaaagt tgtgtctggg aaaagtgcag ggaattcggg ctggcaatat
    12481 acaccgcgtc aatggcatcg ctttccgcca tcgcttccag cgacgtaaac agatgctcga
    12541 cagaaaaatc attggcgaag tgctgagcct gctcaaggct gcgggaatat acggcggtta
    12601 acttgtattt accgctctca tgggcggcct cgacgaactg gcgagtgatc cagttcgtac
    12661 caatcacagc gaaacgtatc ataacgcgtg gatactccat aaagggattc tgtcgtcaat
    12721 gtagcacgcc tttttcatct tcgacgtgcg cagatacgca ttattctttc gccggacagc
    12781 catgctcact aaacggccca atcatgaccg atggcccgag atatttcggc tggcgatgga
    12841 ggatttttgc gtcaatcacc gcgctgacac gagcgagttt ctcccgcagt tgagtataaa
    12901 aaccgtgctt catattgagg tctggcgcgt taaacccgat agcgtcgatg ccgtactgtt
    12961 ttgccagcca gatggtgcgt tggttatgaa attcttgcga gataatggtg atatggtttt
    13021 ccccaaacac ctttttggca cgcactaccg aatccagcgt tgagaatccg gcatagtcgc
    13081 aaaagatcac tttcgccggt acgcctttag cgatcaaagc ctgctgcata cctgatgctt
    13141 catcgtaatt tttacgtccg ttatcgccac tcaccagtag ccatttcact tttccggcgt
    13201 ggtacaacgc tgctgcggta tcaatacgtc gggtaaagta gcgattaccc ggcctcgccc
    13261 ccagcaataa acccacgtta cgcgccggga cggcgttgac atcgccccag gtcagctgct
    13321 tgctggcgtt gaccataacg cgatcggcga taaaaatggt cgctcctgcc acaagtaaca
    13381 gtaagcaggc aatctttaga gtccggcgag aaaaacaaat acggagaaga aggcgggcaa
    13441 atgcgcgcag catggtcgtt atccttaacg aaagggggga ggtcgatttt aggcgctaaa
    13501 gaaagaaaag ataacgcctg acatcatttt cagattttgt cctgcccaag cgacaactgc
    13561 gttagccaca gccgggtgtc gaactcaagc tggtggtact gcggctccat atggcagcac
    13621 aattgataga acgctttgtt atgctctttc tccttcaggt gcgccagctc gtgcaccacg
    13681 atcatgcgca aaaacggttc cggcgcgttg cgaaacacgg tagcgacgcg gatctctgct
    13741 tttgctttca gcttgctgcc ctgcacacga gaaacagcgg tatgtaaccc gagcgcgttt
    13801 tttagcacat ggatcttatt gtcatacatc actttattga tcggcggggc attacgcaga
    13861 aactgatttt tcagatcctg agtatattgc cagagagctt tatcggtggc gtagtcgtgt
    13921 gttcccggat agcgttttgc cagcacatcg cccagacgct gttcgttaat cagtgttcgc
    13981 acctgcgaaa gcagctgctc gggataaccc tgaagataag taagattgct cataaccgcc
    14041 cacgaaaaag agaaaaaggg tatactcacg cacccttttc aggggcaacg tcgaaatttt
    14101 atcattcagg agggccgatg agccacttag acaacggttt ccgttcactg acactacaac
    14161 gttttccggc gacggatgac gttaacccgc tacaggcgtg ggaagcggca gatgaatatt
    14221 tgctgcaaca gttggacgac acagaaatcc tcggcccggt gttgatcctg aatgatgcct
    14281 ttggtgcgtt aagctgcgcg ctggcggaac ataagccgta cagcattggc gactcataca
    14341 tcagtgaact ggcgacgcgc gagaatttac gcctaaacgg gattgatgag tcgagcgtga
    14401 agtttctcga cagcaccgcc gactacccgc aacagccggg tgtggtgctg atcaaagtgc
    14461 cgaaaacact ggcattgctg gaacagcaac tgcgtgcgct gcgcaaagtg gtcacgtcgg
    14521 atacacgtat tattgccggt gccaaagccc gtgacattca cacttccacg ctggaactgt
    14581 tcgaaaaagt gctcggcccg accaccacca cgctggcatg gaaaaaagcg cgcctgatta
    14641 attgcacgtt caatgaaccg ccgctggccg atgcgccgca gaccgttagc tggaagctgg
    14701 aaggtactga ctggactatc cataaccatg cgaatgtctt ctcccgcacc gggcttgata
    14761 tcggcgcgcg cttctttatg caacatctgc cagagaatct cgaaggggag attgtcgatc
    14821 tcggttgcgg taatggcgtt attggtctga cgctgcttga taaaaacccg caggcgaaag
    14881 tggtgtttgt cgatgaatcg ccgatggcgg ttgcttccag ccgtttgaac gttgaaacca
    14941 acatgccaga ggcgttggat cgcagcgagt ttatgatcaa caacgcgctc tccggcgtgg
    15001 agcctttccg ctttaatgct gtgctctgca accctccgtt tcaccaacaa catgcgctga
    15061 ccgataacgt cgcctgggag atgttccact acgcccgccg ctgcctgaaa atcaacggcg
    15121 agctgtatat cgttgccaac cgccatctgg attacttcca taaactgaag aaaattttcg
    15181 gcaactgcac cactattgcg acgaataata aatttgtggt gctgaaagcg gtgaagctgg
    15241 gacgtcgtcg gtaagattgg gagttacctg ccggatgcgg cgtaaacgcc ttatccggcc
    15301 tacgttcagc acgttacccc caggccggat aagacgcggc aagcgtcgca tccggcaatg
    15361 tctgctcagt tctggaatcg cgcttccata aaacaaaaaa tccctgtcac catcctctac
    15421 attctcttgt ttagcgtttt tctacgttta ttcttccgtc acacagataa attcatccgt
    15481 tgccaatctg tcaacggatg ttatcatatt gccaatttgt caacggacgt gatgaagaat
    15541 gcacctgata actcaaaaag cattgaaaga tgctgcggaa aaatacccgc aacataaaac
    15601 ggagttggtg gctctgggga acacgattgc taagggatat ttcaaaaaac ctgagtcatt
    15661 aaaagcagta ttcccatctc tggataactt caaatatctg gataagcatt atgttttcaa
    15721 tgttgggggc aatgaattac gtgttgtagc aatggtcttt tttgaatcgc aaaagtgcta
    15781 catacgtgaa gttatgacgc ataaagaata cgatttcttt accgctgttc atcgtactaa
    15841 ggggaaaaaa tgattgcgat tgccgacatc ttgcaagcag gagaaaagct aactgctgtg
    15901 gcaccttttc tggcgggtat tcagaacgag gaacaataca cccaggcgct ggaactggta
    15961 gatcatctgc tgctcaacga tcctgaaaac cccttgctgg atctggtgtg tgccaaaata
    16021 accgcgtggg aagaatcagc gcccgaattt gcggaattta atgccatggc tcaagccatg
    16081 cctggcggta tagccgtgat tcgtaccctt atggatcaat atggtttaac cctttccgat
    16141 ctgccggaaa ttggcagtaa atctatggtg tcacgcgttt tgagcgggaa gaggaaatta
    16201 acgctggaac acgctaaaaa attggcaacg cgattcggca tttctcccgc cttgtttatt
    16261 gattaacgcg acgtgccgaa tgcgttgcat ccggcgcttc aaataacgat ttaaatctcc
    16321 agcgccagcc gtgttccctg ggcaattgcc cgtcgtgcgt ccagctccat agccacatcg
    16381 cagccgccaa ttaaatgcac agttttcccg ctatcaatca gcggttgcgc cagcgcgcgg
    16441 tttggctctt gccctgcgca gatcaccaca ttgtccactg ctaatacctg cgtttcgccg
    16501 ttgatcacca catgcagccc gtcatcgtca atcttctgat aacttacgcc tgggatcatt
    16561 ttcacacccc gcgagagcag ggtggtgcga tggatccagc cggtggtttt gcctaacccc
    16621 tgtcccggtt tgctggcttt gcgctggagc atcacaattt gccgtgggct acgcgggatc
    16681 tgcattccct gcgggcttaa gccaccagcc tgttgtaggc tgctgtcgat cccccactca
    16741 ttacagaacc tggcgatatt ctggctggtg gattcgcccg gctgacttaa atacatcgcc
    16801 gtatcaaaac caatcccgcc acaaccgatg atggcaacct tgttgcctac cggcgctttg
    16861 tcgcgcagta catcgagata actcaatacc ttcggatgat cgatcccgtc gataggcgga
    16921 acacgcggca cgatcccact ggcgaggatc gtttcatcga acgcctgtaa ctgatccgcc
    16981 gtcacggtgt gattgagttt cagcgtcacg cccgtcactt cgatcatccg gcggtagtag
    17041 cgcagcgttt cgtaaaactc ctctttgccg gggatctgtt tggcgatatt aaactgcccg
    17101 ccgatctcgc tgtgcgcatc aaacaatgtt acctgatgcc cacgcgccgc cgcgttaatg
    17161 gcaaacgcca gcccagcagg tccagcgccg accaccgcca gatttttttt ctgcacggcg
    17221 ggaaggattg gcattttggt ttcgtggcag gcgcgaggat tcaccaggca cgaggtgact
    17281 ttgccaacga agatctgatc gaggcagccc tggttgcagc caatacaagt gttgatctca
    17341 tcggctcgcc ctgattgcgc ttttgacagc agttccgcat ccgcgagaaa cggtcgcgcc
    17401 atcgatacta tatcggcatc gccgcgcgag agaatatcgt cggcaacctg cggatcgtta
    17461 atccggttgg tggttaccag cggcagcgag acgtggcctt tcagtttgcg cgtgacccag
    17521 ctaaatgcgc cgcgcggcac gggcgtggcg atggtcggaa tccgcgcttc gtgccagcct
    17581 attccggtgt tgataatggt cgcgcccgcc gcttcaatgg cctgcgccag ttctaccgtt
    17641 tcggcaaaag tgccgccgcc ttcgactagg tcgagcatcg acaaccggta aataataatg
    17701 aagtcgttgc cgacgcgttc gcgcaccgta cgcactactt ctacggcaaa tcgcatccgg
    17761 ttgcggtaat cgccgcccca ctggtcacta cgctgattgg tgcgcagcgt cagaaattcg
    17821 ttgatcaaat acccttcaga gcccatcact tcgacgccat cgtatccggc ctcccgcgcc
    17881 agttgcgcgc agcgggcgaa atcgtcgatc agttgcagga tctcttcatg ggtgagttcg
    17941 tgaggaacga aacggttgat gggggcctgc aatgcggacg gtgcgaccag atgcggttgg
    18001 tagctgtagc gcccggtatg caaaatttgc agggcgattt tgccgccttc ctgatgtacg
    18061 gcttcggtaa tggtgcgatg gtgtgggatc tggctggcgt cgttgagcat tgcgccacct
    18121 tccatgccaa cgcctgttaa atctggtgca atgccgccgc tgacaatcag cgccacgccg
    18181 tgacgggcgc gttcggcata aaacgcagcc agccgttcag caccgtccgg gtattcctcc
    18241 agcccggtgt gcattgaacc catcaacacg cggtttttta acgtggtaaa acctaaatcc
    18301 agcggggcga acagcgacgg gtagctcata aaattgtcca gtatgtaaaa taattgttat
    18361 gtagtcggat gagttctaat ttagccgtcg atggagaaaa gggaaaaggg gagtgcgggg
    18421 tttgtgatgg gattcaaaaa gaatacgccc ggtggtaacg ccggatgcga cgcttgccgt
    18481 gttttatccg gcttggggga ttgcacatgt agggcggata aggcgttcac gccgcatccg
    18541 ccagccgttg caaattactg cttacggaaa aaatcgttat acaacatata caaatgagcg
    18601 gcactccagg agaaatttgg tgcgccttgc tgcgcgcctg tcagcgggtt gtaattctcc
    18661 tgaattgggc catcggcggt taatcctttg gcgtgcctga agaacgtatc cgccagcttc
    18721 agggcatcat cacgataacc gtaacgctcc atccctttca gaccaaacca gaactgatcc
    18781 acccatacgc gcccgcgcca gtagatatca gcgccaaaag ccggattggt taacgccgcc
    18841 gttcccagcg gaacaaatgt gttgaactct ttaggatcga gcatcacctt caccaccgcg
    18901 tcggcattgg cctgcgttgc cgcaccgtta aacagcggcg accagccttc cggcccttta
    18961 ccgcgctcaa caatcggttt gcccgcgcag ccgttcgcca gtggtttatc ttcaatacgt
    19021 acgtcatagt agaactgggt agtcgggtcg aacatacagg tgttgatgta gtccgcgagc
    19081 tgctgtgcca actggcgata gcgtttggcc tcttctggct taccgaggat ggttgccatc
    19141 tccgccagat aatggttatc gctgtacata tagctggcct gatccaccga ctcctgcaat
    19201 agcgagtagc ccagcaacgt tccgtcctga ctgcggtttt cggcgaattt caccgtccag
    19261 tcgctacgtt tgccgccatt agcgacatat ttatccaact gttctttgtc gataaaacca
    19321 aagacggcgg catcatcacg ccccgattcc cacgacgcag cgacctgtgc cggaatttcc
    19381 agactgtcgt actggccttt ctccaccacg cgggcgtagt tgttcagccc agactgcgtc
    19441 tcttctttat cgcctttttt caccgtaaac agcatctcgc cgctctcggt gttgtgggct
    19501 ttgtcgcggg tcgcgccata ttccggcacc ccgttgccgt tatgatcgcg gttacgtaac
    19561 caccagtcgt gataggccac cagtttcggg tacatctctg ccaaccaggc tttatcctgg
    19621 gtgacgttgt atacttccat caccgaccag gcggcaaggc tgggtttggt attacgttcg
    19681 ttccagttgc cgccatcgcc accacgctcg gggctaaggt tccacgctat caggtcgggg
    19741 acaaagccca catcctgcgg acgcacgctg tcgccaggct ggatctgcca ggagaagacc
    19801 gcgcggatat tctctttggc gatatccgga ttgaaatgcg ccatcgcaaa cgcctgcttc
    19861 caggtatccc acggccaggt ctgattgccg gagaaccagc gtccggtcac cgacggcgtg
    19921 acggtgttat aattcaccgc accgcccggt gagcgccagt taccgttgag cgtttcgatc
    19981 gctttcaccg cgacgcgcgt ctgttccggc gtcgcatccg gattggttaa ccctttcttc
    20041 aggtattctt cccagcgttg ctgcgaggcg gtgagataaa acgccggacg cgccagaata
    20101 tcgcggatct gcatttgctc tttgctaact tcctgagcgg tcagcagatg ggaataggtg
    20161 gtgtagagcg tggtcgaacc gttgatatgc gccttactgg taaagcgatt accgttgatt
    20221 tcagtctgca ccggcaggga tttatgcacc tgatattccg attcgccgga ggtcagcaga
    20281 tcccaggtgg cgcgcacttt gccaaaggtg actttcaggc catcacgggt ggcgctgatt
    20341 ttgcgctgat agtcagggta ttcgccagca atggttttat cggaaagcgg tttcccttct
    20401 ttcgcttcca gtttttccag cagttcgcca tcccacacca gatccagcgg tttattgctg
    20461 atgattttag tttccagtag tgacgtgcgc ggcgtggcga agcgcagagt catttcgacc
    20521 tgcacatctt ttgctgtcag tttttgcacc agcgcgccgg gaatactgta tgcctccagc
    20581 gtgaagttga ctttcttgcc gtcttgccag acagtcaggc ggtcgaaatt gctggccata
    20641 aagttgatgt attcttccgt cagcagcgca acgcccggaa agccgcccat ggtgttcggg
    20701 ccgtctggca acagatgacc atgccaggct ccgagatcga aaaacggatt aaaacgctgg
    20761 tgatcgtcgt aatcgtaatc cttcatgtac tgcggcgcac cagtacggtt aatcacgttt
    20821 ttataattgt cggcgttagc ggcatgagcg gaaaaactta tcagcagagc gcaggttact
    20881 ggcgttaaaa tagttttgat tttcatgaga ttacagtcct tattaccagt agaaatattg
    20941 catcaggaag atttggtcgc tgggatcgtt agaggcattt ttcatgtaga agcggctatc
    21001 aaaagccgtg gcgaaacgtc cgccgccata ttcgtaccag ataccaaact gcatgaaaga
    21061 ggtggtttca tcgtcagcgt ggtcagggcg atgtttggcg taggtgtaag cggtggagag
    21121 ataaacgcct tttaacgctt catacatggc gctcaccatc atcccgcttt cacttcccgg
    21181 tacgtccagc ccgtcggcac gtccggtatg gtgccaggcg cgagcggcaa agttaggggt
    21241 aaacaggccc gtgagataga gttgtcctgt gccgtcggta atttccagac cattcatcca
    21301 ggtgatgcct tcggcaaggt catactggat gtaaccgttc atccattgcc ggataggtgt
    21361 atttgtcgtc gtagcgatcg tacttgccaa aatgtagcca ggctttgctt tcgtcatgat
    21421 gtccggcagg tgtggcggtg acgctataac gaaagttgcc gcttaagttc tgtaccttca
    21481 gcgcggtcat aatgtcgcgg gtattgggaa tgacgtagcc caaatcgggt gtgaagtcgc
    21541 cccaccattg caggtcatcc agcgaggaat cctgacgcag actgagcatg atttcggtgc
    21601 catcattggt acgataaccg ccatagaaac ggttaatccc accttcgaaa ccaccccagc
    21661 tatggtcagg cacctaggca ttaccgtcgt ggtcggcctg cacggtccag ccttcgccgt
    21721 aaatcaggcc gaaccaggag ccgtacttca tctccaggcc accttcaata taggtgccgt
    21781 cgctgtattt atgtttattc tcaccctcta aatccatata tccaccaagc cctaattcgc
    21841 cgtaaaaacg aaatgaagga gaagatgtcg aggtaattgc gggtgttttt gtgtcgtcat
    21901 gttctgccgg acgtgcttct tctcctgccg cagaaaaggc gtagcaaaac ggcagagcaa
    21961 gtaatgctct gcatggtgca gtgattaatt tcattgtgtt aatccatgag ctaaagaaaa
    22021 agtacagcct tttatcgttg taattataaa aatataaagg cagaaactat tttccctctc
    22081 ctcatccagg aagagggaaa atgcctgctc attttttatt agtctgctta atcaacattg
    22141 ctggctggcg tcgcttcaat atggcgctct tcagccgtta atccctttat atatttgctg
    22201 tatttccacc acgcaaagcc gaggaagata acaataccgc cgacgttata aaaaatgatg
    22261 gtcagaatat tcgcgccagt cgggaatgtc gaagcgacaa accctacggc aaatatcgca
    22321 atcagcattg aaacaacgat aataccggtg cggcgggagc ccatacggaa atcgcgtggc
    22381 aggtgatcta atttggcgcg taaattcagg taagccagca tgatgaataa cggcggaagc
    22441 atggacgctg cggcggtcat attaataatg gtattcatca gatcctgcac ggtattggaa
    22501 ccgagcatcg ggataatcat cagcgggatg acgatcagaa actggatcca tgctgcgcgc
    22561 gccggaacgc cgttttcgtt aagttcgacg gttttcttac caaagatgcc ttccgggatt
    22621 tcggagaaga aaattttcac cggcgttgcg gtccacatca gcagggaacc gaacattgcg
    22681 gtaaaggaca ccagaccgac aaagcgattc atcaacgctt ccggcagacc aaaatacgcc
    22741 gccatgccgt ggaatacctg caccgatccg ccggtaaatt tcaactcttt gctgctgacg
    22801 aagacgttaa tcagcaccga ggagacggaa tacagtacgc cgataaaaat cccggcgagg
    22861 atgatcactt taacgaacga tttcgaaccg cctttgacgt cgttaacgta caccgcgacg
    22921 gactccgcgc cacctgcggc cataaagatc caggtggtaa cgccgaggaa cgcccagttg
    22981 aagttcggga tcatcgcgtc aacggtgatc ggatcggcag gctgtacgcc gccaaccagt
    23041 gccgtacctg ccagtaaaat gtaggagagc gttaacagca gcatcagcgt tgaagtgacg
    23101 gaggtaattg gccccagcat tttcgccccg ttggtggaaa cccaggtgga gaaggcgaac
    23161 agcaccatac tgataacggt ggtggcaacc ggcgtcataa tatattcgta gccgaggaag
    23221 gcatacgaag cataagcaat aacgcgcggc aacagcgagg tgaaaaagaa cagatttacg
    23281 aaccagtaag tgtaggcagt aataaatgcc caacgtccgc ccagcgaact ttttacccac
    23341 gcgtagacac cggcttctga gtttttattt aacgaaacaa attctgcgat gatcagacag
    23401 aagggaataa aataaaaaat cgtcgcgagg aaaaacatcg gtgccgaggc cagtccaagc
    23461 tcaatattat tattgataac gttgttaaag ctgtaaacgg cggcaaaagt cagcgagagc
    23521 aagccgaatt tgccgattgt attacgtttg gtatcagaca taacacatct ccgaaaatgt
    23581 gtagggtagc ggtggcggca aactcctttt gccgccgtag ttgttatttg ttatggaaat
    23641 aaccatcttc gatggtgact ttgagaacca cttttttgac cgcgttattg cagataaacc
    23701 gatacgcttc atggatatcg caaataacga tttgcccttc gtggacctct acggtttctc
    23761 cgcagccttt taaatattca cggtcagttt catcacgata atattccact acctgtaatg
    23821 tctctttcgg cgcatattca attttttgct gcccttgcag gtagtaatgc acttcaaaat
    23881 agcgtcgatg cccggtaaat agcgcatcgg tcgcggagtc attttccaca cggtaggtca
    23941 atgaatcacc gatggagtgg gcgacgccag gctgaatgtt gtcgatattt tcaatcgctt
    24001 caacgcagcg ttgccacttc ttgccagagg cgtaaatctg gcggaactgt tctaagttat
    24061 cgatgatcct cattgcttat tctcgctgtg caaattcgtg gaaaagaacc ctgcgccgaa
    24121 ctcatacgac gccaggcttt gcgcggtagc ttctccgcca gaaaccggca gcaacgtaaa
    24181 gccgtagctg aagtcacgga accagacgcg ccaggagtcc agcacctcgc tgccccagga
    24241 gttggagccg aggccaagta gctggtggtc gaggttcagg gtgatgtcat cactgcgctg
    24301 cagctcgtta cagtgctggg cagcgtggat gttttcctgg gtatagtgcc aggcgctgaa
    24361 gttaattggg cgctgcggaa ccaccagcag accgttgccg tggcggttag tcagtgccgt
    24421 ccagcggaca tgctgacggt tgccgttgtt ctgcgggaag ggatagttct cgaacatggc
    24481 atcgacggtg ctgcgccaga tatcgatgat gttagcctgc tggctgtcgg cgtagttttc
    24541 gcccggtcca cgaccgtaat acgccacctg atcgtattcg ccgttaatcc ccatggtgaa
    24601 accgatgcac ggaatgatgt gcggatagtc gccgtaacgc tcgccggaaa gcgccacgtt
    24661 aacctggcca tcggcagcga tgcgccagat gtaggtacag cgcatcccga agtcaaacac
    24721 cggcggtgca ataactgtgc ggctgatgat caacacttca ccatcgctct gttctacggc
    24781 aaagtcgcgc agatgttcct gcatgatctg caaatgattc ggctgccaca acccttcgta
    24841 ctcctgcttg tggttgtcga tcatcggctt gaagaagttg atctttggct cgcgggtcag
    24901 cagcgactcg ccatttacct gccaggatgt cggtttgcca ctcatttttg agaaggtgat
    24961 cgcgaagttg tagccgcgaa cggtgcagct caaacgatcg tcttccagcg tcagcggacg
    25021 cgcattattt ggtgcgaaag gcactggctg cgcggtgttt tccttcagcg ggaactgata
    25081 agtggcgata gaatgcccgg cttcgctgta gcgggtgcgg gaatctttgg tcaccgtaat
    25141 gttgaggaac gcttcgcggg cgtccagctg cggcagcgtg atctgcaagg gggcttcgct
    25201 gttcggcgca acgtcgcgca gtttaatctg ctgcgtcgcg agcgtttcac cttcggcgcg
    25261 cacctctgcg tgcagggtgt agtcatcaag cgtggtaaac cacagtttat tttcgacttt
    25321 caactcgccg cgagtcagcg cgtggatttt taccggcgcg ataacctgtt tgtactcttt
    25381 caggcccgat cccggcgtct gatcggaata gatcaaacca tcaagacaga agttatagtt
    25441 gttgggatag tcgccgtagt cgccgccgaa tttataccag acattgccgt ggtcgtcctg
    25501 tgcctggatc ccgtggtcgc accactccca gacataatga ccctgaatgc aatcgtgctt
    25561 atagaagacg ttctggtact ccgtcagccc gcccggtccg ttccccatcg catgagcata
    25621 ttcacagatg atgcgcggct tcggatgcgg gtattcacca aactcattca tcagcggcac
    25681 gcgggtgtac atggtggaaa taatatcgac cacttcagca tcgcgatctt cttcgtaatg
    25741 aaccagtcgc gtgtcatcca gcgctttcgc cgcatggtac atcgcgcgga tgttacagcc
    25801 atagccggat tcattgccca gcgaccagat gatgatcgac ggatggtttt tctgtgcgtg
    25861 gatatggcga acaatgcgct cgacgtagac tttttcccac tgcggatcgt cggtaatacg
    25921 gctgatatcg ccgacattag caaagccgtg cgattcgacg tcggtttccg ccatcacaaa
    25981 caggccgtag atatcacaca gttcgtaaaa acgcggatcg ttcgggtagt gagcggtacg
    26041 cacggagttg atattgtgct gcttcatcaa ctggagatct ttctcgacgc gatccattcc
    26101 aacggcgcgg cctttgcgat gatcgttgtc gtgacggttg acgccgtgca gcatcacata
    26161 acggttattg atccagaaca gaccgtcgcg cactttgata tcacggaagc caacgcgttg
    26221 tggcaccact tccagaacgt tgccgttggc gtctttcagc gtcatgacca gatggtaaag
    26281 ataaggggat tctgctgacc attgctgcgg ctgttcgaca gtaaaagcaa agctggcgct
    26341 ggtcagtttt tcaattgcca aatgatcaat ggcgctgctg tgcaccacgc gttcgccatc
    26401 aaacagggta tattccagcg tcgtgacgac aggggaggcg gcgagatttt ccagcaccac
    26461 ttcgcaggaa agcgtggcat cgcaataggc ttcgtcaaag tcggtacgca cagtgaaatc
    26521 gttaatatgc gttaggtgtt ttccgaccag ataaacatcg cggaagatcc ccgccgacca
    26581 ccacatatcc tggtcttcca cgtaggtaga gtccgcccac tgcatcacgc gcacacacaa
    26641 caggttgtcg ccggttttaa ccatcgcgct gatgtcaaac tctgcggtca ggcgactgcc
    26701 cttgctgaaa cccacatact gaccgttaac gtagacttca aaataggttt cgacgccgtc
    26761 aaatttaatc agcgtctgtt taccctgcca gccgtcgctg agggtgaaaa tacgttgata
    26821 ggcaccggtt gggttatcgc tggggacaaa cggcacatcg atggggaacg gaaaaccttc
    26881 gtcggtatat tgcagtttgc cgtgaccttc catttgccac atggcgggga cggtaatatg
    26941 cccccagtca gccattaact cagaggtgaa ggcttctggt acttgcagcg gatggtcaaa
    27001 aaagtggaaa ttccactgac tgcttaaggg cagaaacagg ctgctggttt cgcgggcaaa
    27061 ggtacgcgct tgcgcaacag aatcatatga aaaaaagtac gcacgcggcg caagtcggtt
    27121 ttcgtgggtg agctgaatgt tttcccagcg attcataggg cctcccggta gagagaaatc
    27181 ggaaccattc atggtgatgg tagtttgtgt aattgctggg ggtttagtaa aatttttact
    27241 aaaaagcagc aggtaaatgc atttacttta actggatcac gaaaataaac ggcagcactg
    27301 tccggcaaaa atgattgccg aacagtgtgt aaggggattt aacgggtcgt gccgcgcagt
    27361 tttaatttgc tgggaacgaa gactagcagc ggcagcgcgc gaccatcgcg ggctttttca
    27421 tacaccaggt taacgccctg acttcccatc atctcggaat ggatgcgcac ggtggagagc
    27481 ggcggaaagg taaatcgcgc ggtggggata tcgttaacgc tgataagcga aatatcttgt
    27541 gggatgttca ggccacgttc atgaattgcc cgcagtacgc cgatagcaat ggaatcggaa
    27601 gcaacaaaca gtgccttcgg atagtcttcc cgcgccagca tttgttttgc cagttcataa
    27661 cccgacgaac tggaaaaacc gccgcgccag atatcctctt cgcgtaccac ttgtttcagt
    27721 cggccatatt ccgcaaaggc gacctcacga atatccgcct tgccaggctc atcttcaccg
    27781 ccaataaaac caatacgatt aacgccctgg ttgatataga agtcgatgat ttctttactg
    27841 atgcgtgcca gatcgatatc caccgcatcg taaccgctgc cgggttcgtg aaagtcgata
    27901 aaacagatat tgtcggtcaa cgcgctggca gcggcgcgca gggcgggcgt gggtttgccg
    27961 acaattaaaa taccggtgac gtttttaatg tctggtaatc cgttgtgttc ataacagttg
    28021 gtcagttcga tcgccagctt ttcgcactga gtttcaatgc cgtggcggat cgccagatag
    28081 taaggatcgt tgatctccag ctcctgctgg tagctgtaga tagccagaat atggtgttgg
    28141 ttgactgcgc ctgtctgtag tttacgggcg ctactggtct tgtactccag cttttcggcg
    28201 atctcgagaa tgcgatgttt cgtctcttct ttcacattca atgtcggatc gtcatttaag
    28261 accctggata ctgtcgccag ggatacgcca gcttcgattg cgatgtcttt taatgttgcc
    28321 atgtttacct tttttacttt ccctgatgtg cgcccccatt gtagaggaag tgcttatcgg
    28381 gtcacctgtt ttcagtaaat aaagcgcgtt tctttgacgc tgtaacggta acacagcggc
    28441 ttaggaatgt gatcacgcct gcattctcca tttttagcct acaggattga gaaaaacttt
    28501 tctatagttt agtaaaattt tactcgaagg gaggaagcaa tggaaaccgt ggcttacgct
    28561 gattttgcac gtctggaaat gcgcgtcgga aagattgtgg aagtgaaacg ccatgaaaac
    28621 gccgacaagc tgtacatcgt acaggttgat gtggggaaaa aacactgcaa accgtgacca
    28681 gcctggtgcc gtactacagc gaagaggagc tgatggggaa aacggtggtg gtcttatgca
    28741 atctgcaaaa agcgaaaatg cgtggtgaaa cgtcggaatg catgctgttg tgtgcggaaa
    28801 ccgatgacgg cagtgaaagc gtgttgttaa cgccggagcg gatgatgcct gcgggcgtac
    28861 gcgtcgtata aaaagatcgg atggcgacgt cgtatcgcca tccgatttga tattacgctt
    28921 cttcgacact tactcgcatg gccgccagcg ccttacgcgc cgctttgatc accagttcac
    28981 actgttcaat ggtcagtgtc agtggcggtt caatgcggat cgttttggcg ttattgagcg
    29041 ttccggccac cagtacgcgc tggcggaaca tctcgctggc aaagttatag ccgatttcgt
    29101 tatcaacaaa ctcaatcgcc atcaacatcc ctttaccacg cgcttcctgt accagatcgg
    29161 gatattcccg cgccagttga cggaaaccgt ccagcaacat atcgcctttt tgctcagcct
    29221 gagccggtaa gttctgctcc agcaacacat tgatggtcgc cagcgccgcc gcacaggcca
    29281 gcgggttgcc gccaaaggtg gtggtatgca ggaatgggtt gtcgaacaga actgaaaaca
    29341 cctcttcagt ggcgatggtc gcgccaatcg gcatcacgcc gccgccgagg gcttttgcca
    29401 gacacagaat gtccggctga acgttctcat gttcacaggc aaacatcttg cccgtgcgcc
    29461 ccatgcccgt ttgtacttca tcgaggatca tcagtgcgcc gaactcatcg cacagcttac
    29521 gcacggcggt gagatagccc ggcggcggca gaattacgcc accttcgccc tgaatcggtt
    29581 cgaggatcac cgcagccaca tcatcaccgg tttttttgca ctcattaaga gccgtgcgca
    29641 tggcttcgat attgccaaac ggcacatgac ggaaccctgg cagcagtggc ataaacggtt
    29701 tgcggaaggt cgatttcgcc gtggccgaca gcgcgccaag tgatttaccg tggaacacgc
    29761 cgctggtggc aataaaagta aatttgccgc gcggcgactg ataagccttc gccagcttca
    29821 gcgccgcttc aacggactcg gtgccgctat tacagaagaa gctgtatttc agtttaccgg
    29881 gcgttagcgc agcaagggtt ttcgccagca tagcccgtaa cggatcgagt agctcctggc
    29941 tgtgcagtgg ttgtttcgca agttgattct gtacggcgga aaccacaact ggattacggt
    30001 gccccacgtt gaaaattcca aaacctccca ggcagtcgat aaactcctgt ccctgggtgt
    30061 cgacaagcgt atttaaacct cccgcttgcc actctacggc tccgtaatcc ccgccggcgg
    30121 taacagattt gcgatactct aaaaaccccg gattgacatg ctctttgaag tattcaatca
    30181 cctctcggtt aagtgctttc atctcctcat gatccagcgt tcgcttctca atgagattca
    30241 gggcgtgggc gctgcacgct aaagccgatg cgctcgaagg taacctgttc aaaatatgct
    30301 ccggaggctc gcgtatcaca tgataccgat ttaagtattg cagggattgc gccactccgg
    30361 ctgcgatcgc gaaaatcggg ataaacacaa atgcggttat catttgtgca atatttaatg
    30421 ctatccgtta acatttgttg ttattttgca cggtttttgt cgggtatgga tttgatgatt
    30481 gctgcgcaaa tgcacgtttt cagggcaata attgcctgat ttttgggcaa gataattaac
    30541 gttttcgtat agtaacagag tgaaagtatt ttaaatcaaa gaattagatt tgtgctttaa
    30601 tcgcaaattg cgatctaaat caaattaatc ggttaaagat aaccgcagcg gggccgacat
    30661 aaactctgac aagaagttaa caaccatata acctgcacag gacgcgaaca tgtcttctca
    30721 tccgtatgtc acccagcaaa ataccccgct ggcggacgat accactctga tgtccactac
    30781 cgatctgcaa agctatatca ctcatgctaa tgacactttt gtgcaggtga gcggctttac
    30841 cttgcaagag ttacaagggc agccgcacaa catggtgcgt cacccggata tgccaaaagc
    30901 ggcgtttgcg gatatgtggt tcaccctgaa aaaaggggag ccctggagcg gcatcgtgaa
    30961 aaatcgccgc aaaaatggcg accattattg ggtgcgggcc aatgcggtac cgatggtgcg
    31021 cgagggaaaa atcagtggct atatgtcgat tcgtacccgg gcgacggatg aagagattgc
    31081 ggcggtggag ccgctgtaca aagcgttgaa ctccggacgt accagtaagc gtattcataa
    31141 aggcctggtg gtgcgtaaag gctggctggg taaactgcct tcattaccgc ttcgctggcg
    31201 gacgcgtgga gtgatgaccc tgatgtttat cttgctggcg gccatgcttt ggtttgttgc
    31261 tgccccggtg gtgacgtatt tcctctgtgt gttagtggta ttgttggcaa gcgcttgttt
    31321 tgaatggcag attgtgcgcc cgatagaaaa tgtcgcccgt caggcactga aggtggcgac
    31381 cggagagcgt aatagtgttg agcacctgaa tcgcagcgat gagctggggc tgacattacg
    31441 cgcggtaggg cagcttggcc tgatgtgccg ttggctaatt aacgatgtct caagccaggt
    31501 gtccagtgtc agaaatggca gtgagacgct ggcgaaaggc accgatgaac tgaacgaaca
    31561 tacccagcag acagttgata acgttcagca aacggtggcg accatgaacc aaatggcggc
    31621 gtcggtgaaa cagaactctg ccacggcgtc ggctgccgat aaactttcta tcaccgccag
    31681 taatgcggca gtgcagggtg gggaggcgat gaccacggtg atcaagacaa tggacgatat
    31741 cgccgacagt acctagcgca ttggcaccat tacttcgctg attaacgata ttgcgtttca
    31801 gaccaatatt ctggccctga atgcggcggt ggaagcggcg cgtgccggcg aacagggcaa
    31861 aggttttgca gtggtggcag gggaagtgcg tcatttagcc agccgcagcg ctaatgctgc
    31921 caacgatatt cgcaagctga ttgatgccag tgctgataag gtgcaatccg gttcgcagca
    31981 ggtacacgcc gccggacgga cgatggaaga tattgtggca caggtgaaaa acgtcaccca
    32041 gttgatcgcc cagattagcc attcaacgct ggaacaggcc gatgggcttt ccagcctgac
    32101 ccgtgcagtg gatgagctta acctgatcac ccagaaaaat gccgagctgg tggaagagag
    32161 tgcgcaggtg tcggcgatgg tgaaacaccg cgccagccga ctggaagacg cggtgacggt
    32221 gctgcattaa tcggtatgcc ggatccggcg gttggagcac aatgcctgat gcgatgctgg
    32281 cgcatcttat caggcctgcg gggtgtgtag cgggtgtagg ccggataagg cgcgtcaagc
    32341 gtcgcatccg gcaattgcac cgcgccactg gcggatgcgg cgattaatcc agttgcgtaa
    32401 tatcaaaagc ggcgcggtcg atcaccgcaa tgatcttttt tatttgtgca tcactgatat
    32461 cgctctggtt gacgcgtaaa tccagcaccg ctttaaaatt atccagcgcc cgcttcattt
    32521 gcgggttctg gcgcagcgcc gcgccaacgc aacgcgcttt gatgcgttct tcaatcatct
    32581 ccacttgttc gcggttttct tccagccact gcgcgccttg ttcggtcagc gcaatctgct
    32641 tcttaccacc ttcctcttcg cggatggtaa tcagcgactg ctcctgcaga aaatccagcg
    32701 tcgggtagat gacgcccggg cttggggtgt aattcccctg ggttagattc tcaatcgctt
    32761 taatcaattc gtaaccgtgg ctgtcatcgc gcgagagaat atccagaatc accagacgca
    32821 attcaccgtg accaaagaaa cgttgccgac gaccgccgcc gcgaccgtgg cgaccaccgc
    32881 aacattgacc gtgttcatgc tggtgaccgt gtccgcagtg ctcgtgttct gacttctcac
    32941 ctttgcagca gccctcatgg cgtggctggc cttcatgttt acaacaccct tcgtgatgat
    33001 ggctcatatc agcctccttt tgcttgatta gatatatcta atttatatct gatgtgagca
    33061 ttactgcaag tacaaaaatg atttttaaac gtggaagcac ctcacatcta ggccggataa
    33121 gacgttcacg ccgcatccgg cagcattgct atggcactga atgccttcac atacttcaga
    33181 catatcagtt ctacaaatcg cttgcattta tcatgattaa caatcattat catttgcgaa
    33241 ttttatttag atatatctga ttaccatcac gaaggcgata acaatgaata acaccccccg
    33301 ctacccgcag cgcgttcgca atgatctgcg cttccgtgaa ctgactgtgc tacgcgttga
    33361 gcgcatcagc gccggttttc agcgcattgt ccttggcggc gaggcgttgg acggttttac
    33421 atcgcgcggc tttgacgatc acagcaaact cttctttcct caacctgacg ctcactttgt
    33481 gccgccaacg gtaacggaag agagcatcgt ctggccggaa ggaccacgcc caccgtcgcg
    33541 tgactatacg ccactgtatg acgaactacg ccatgaactg gcgatcgatt tctttattca
    33601 cgacggtggg gtcgccagcg gctgggcgat aaacttacgg tggcaggtcc gcgcggttcg
    33661 ctggtggtgc cggaagatta cgcgtatcag ctgtatgtct gcgatgaatc cggaatgccc
    33721 gcactgcgcc gccgcctgga aacgttgagc aaacttgccg ttaaaccgca agttagtgcg
    33781 ctggttagcg tgcgggataa cgcctgtcag gattatctcg cgcaccttga tggttttaat
    33841 atcgaatggc tggcacatga tgagcaggcg gtagatgcgc gtctggcgca gatgcaaatc
    33901 cctgccgatg attacttcat ctggataacc ggcgaaggta aagtcgttaa gaatttaagc
    33961 cgccgctttg aagcggaaca gtatgaccca cagcgggtac gtgcagcggc ttactggcac
    34021 gcaaagtaac tgatatataa aaagaaaaag gctgacgatt tctcgtcagc ctttgctata
    34081 tctggtggcc cctgctggac ttgaaccagc gaccaagcga ttatgagtcg cctgctctaa
    34141 ccactgagct aaggggccat ggtagcggat tataaagtaa ctccgtgccg caatccagcc
    34201 attaccgcgc gcctgctgtt tttataaaca atgtattttc aatccgttat acttttcctg
    34261 gtgatgttag aaaggagtaa atatggttga ggatattttg gctccagggt tacgggtcgt
    34321 gttttgcggt atcaaccctg ggctttcatc cgccgggact ggttttccct ttgctcatcc
    34381 ggcaaatcgc ttctggaagg tgatatatca ggccgggttt accgaccgtc agttgaagcc
    34441 gcaggaggca cagcatctgc tggattatcg ttgtggcatc accaaactgg tagaccgtcc
    34501 aacggtgcaa gccaatgaag tttcaaagca ggagctgcac gcaggtgggc gtaagctgat
    34561 tgaaaaaatt gaagattatc agccgcaggc gttggctatt ctgggcaaac aagcatatga
    34621 acagggattc agctagcgcg gtgcacagtg ggggaaacaa acgctcacca ttggttcgac
    34681 gcagatttgg gtgctgccaa atcccagcgg tttaagtcgc gtttcactgg agaaactggt
    34741 tgaagcgtat cgcgagctgg accaggcgct ggtagtgcgt gggcgataaa aaacgtcacc
    34801 g
//
LOCUS       DAJTRC010000034        34583 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.034, whole genome shotgun sequence.
ACCESSION   DAJTRC010000034 DAJTRC010000000
VERSION     DAJTRC010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34583)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34583)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34583
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.034"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            857..3142
                     /gene="nrdA"
                     /locus_tag="OO357_001781"
     CDS             857..3142
                     /gene="nrdA"
                     /locus_tag="OO357_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase subunit
                     alpha"
                     /protein_id="HCR5886749.1"
                     /translation="MNQNLLVTKRDGSTERINLDKIHRVLDWAAEGLHNVSISQVELR
                     SHIQFYDGIKTSDIHETIIKAAADLISRDAPDYQYLAARLAIFHLRKKAYGQFEPPAL
                     YDHVVKMVEMGKYDNHLLEDYTEEEFKQMDTFIDHDRDMTFSYAAVKQLEGKYLVQNR
                     VTGEIYESAQFLYILVAACLFSNYPRETRLQYVKRFYDAVSTFKISLPTPIMSGVRTP
                     TRQFSSCVLIECGDSLDSINATSSAIVKYVSQRAGIGINAGRIRALGSPIRGGEAFHT
                     GCIPFYKHFQTAVKSCSQGGVRGGAATLFYPMWHLEVESLLVLKNNRGVEGNRVRHMD
                     YGVQINKLMYTRLLKGEDITLFSPSDVPGLYDAFFADQEEFERLYTKYEKDDSIRKQR
                     VKAVELFSLMMQERASTGRIYIQNVDHCNTHSPFDPTIAPVRQSNLCLEIALPTKPLN
                     DVNDENGEIALCTLSAFNLGAINSLDELEELAILAVRALDALLDYQDYPIPAAKRGAM
                     GRRTLGIGVINFAYYLAKHGKRYSDGSANNLTHKTFEAIQYYLLKASNELAKEQGACP
                     WFNETTYAKGILPIDTYKKDLDAIANEPLHYDWEALRESIKTHGLRNSTLSALMPSET
                     SSQISNATNGIEPPRGYVSIKASKDGILRQVVPDYEHLHDAYELLWEMPGNDGYLQLV
                     GIMQKFIDQSISANTNYDPSRFPSGKVPMQQLLKDLLTAYKFGVKTLYYQNTRDGAED
                     AQDDLVPSIQDDGCESGACKI"
     gene            3376..4506
                     /gene="nrdB"
                     /locus_tag="OO357_001782"
     CDS             3376..4506
                     /gene="nrdB"
                     /locus_tag="OO357_001782"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006683700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleotide-diphosphate reductase subunit
                     beta"
                     /protein_id="HCR5886750.1"
                     /translation="MAYTTFSQTKNDQLKEPMFFGQPVNVARYDQQKYDIFEKLIEKQ
                     LSFFWRPEEVDVSRDRIDYQALPEHEKHIFISNLKYQTLLDSIQGRSPNVALLPLISI
                     PELETWVETWAFSETIHSRSYTHIIRNIVNDPSVVFDDIVTNEQIQKRAEGISSYYDE
                     LIEMTSYWHLLGEGTHTVNGKTVTVSLRELKKKLYLCLMSVNALEAIRFYVSFACSFA
                     FAERELMEGNAKIIRLIARDEALHLTGTQHMLNLLRSGADDPEMAEIAEECKQECYDL
                     FVQAAQQEKDWADYLFRDGSMIGLNKDILCQYVEYITNIRMQAVGLDLPFQTRSNPIP
                     WINTWLVSDNVQVAPQEVEVSSYLVGQIDAEVDTDDLSNFQL"
     gene            4506..4760
                     /gene="yfaE"
                     /locus_tag="OO357_001783"
     CDS             4506..4760
                     /gene="yfaE"
                     /locus_tag="OO357_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin-like diferric-tyrosyl radical
                     cofactor maintenance protein YfaE"
                     /protein_id="HCR5886751.1"
                     /translation="MARVTLRITGTQLLCQDEHPSLLAALESHNVAVEYQCREGYCGS
                     CRTRLVAGQVDWIAEPLAFIQPGEILPCCCRAKGDIEIEL"
     gene            complement(4814..5464)
                     /gene="inaA"
                     /locus_tag="OO357_001784"
     CDS             complement(4814..5464)
                     /gene="inaA"
                     /locus_tag="OO357_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide kinase InaA"
                     /protein_id="HCR5886752.1"
                     /translation="MAVSAKYDEFNHWWATEGDWVEEPNYRRNGMSGVQCVERNGKKL
                     YVKRMTHHLFHSVRYPFGRPTIVREVAVIKELERAGVIVPKIVFGEAVKIEGEWRALL
                     VTEDMAGFISIADWYARHAVSPYSDEVRQAMLKAVALAFKKMHSVNRQHGCCYVRHIY
                     VKTEGKAEAGFLDLEKSRRRLRRDKAINHDFRQLEKYLEPIPKADWEQVKAYYYAM"
     gene            5643..5885
                     /locus_tag="OO357_001785"
                     /pseudo
     CDS             5643..5885
                     /locus_tag="OO357_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311149.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            complement(5927..7003)
                     /gene="glpQ"
                     /locus_tag="OO357_001786"
     CDS             complement(5927..7003)
                     /gene="glpQ"
                     /locus_tag="OO357_001786"
                     /EC_number="3.1.4.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708129.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HCR5886753.1"
                     /translation="MKLKLKNLSMAIMMSTIVMGSSAMAADSNEKIVIAHRGASGYLP
                     EHTLPAKAMAYAQGADYLEQDLVMTKDDHLVVLHDHYLDRVTDVADRFPDRARKDGRY
                     YAIDFTLDEIKSLKFTEGFDIENGKKVQTYPGRFPMGKSDFRVHTFEEEIEFVQGLNH
                     STGKNIGIYPEIKAPWFHHQEGKDIAAKTLEVLKKYGYTGKDDKVYLQCFDADELKRI
                     KNELEPKMGMELNLVQLIAYTDWNETQQKQPDGSWVNYNYDWMFKPGAMKQVAEYADG
                     IGPDYHMLIEETSQPGNIKLTGMVQDAQQNKLVVHPYTVRSDKLPEYTPDVNQLYDAL
                     YNKAGVNGLFTDFPDKAVKFLNKE"
     gene            complement(7008..8366)
                     /gene="glpT"
                     /locus_tag="OO357_001787"
     CDS             complement(7008..8366)
                     /gene="glpT"
                     /locus_tag="OO357_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate transporter"
                     /protein_id="HCR5886754.1"
                     /translation="MLSIFKPAPHKARLPAAEIDPTYRRLRWQIFLGIFFGYAAYYLV
                     RKNFALAMPYLVEQGFSRGDLGFALSGISIAYGFSKFIMGSVSDRSNPRVFLPAGLIL
                     AAAVMLFMGFVPWATSSIAVMFVLLFLCGWFQGMGWPPCGRTMVHWWSQKERGGIVSV
                     WNCAHNVGGGIPPLLFLLGMAWFNDWHAALYMPAFCAILVALFAFAMMRDTPQSCGLP
                     PIEEYKNDYPDDYNEKAEQELTAKQIFMQYVLPNKLLWYIAIANVFVYLLRYGILDWS
                     PTYLKEVKHFALDKSSWAYFFYEYAGIPGTLLCGWMSDKVFRGNRGATGVFFMTLVTI
                     ATIVYWMNPAGNPTVDMICMIVIGFLIYGPVMLIGLHALELAPKKAAGTAAGFTGLFG
                     YLGGSVAASAIVGYTVDFFGWDGGFMVMIGGSILAVILLIVVMIGEKRRHEQLLQKRN
                     GG"
     gene            8639..10267
                     /gene="glpA"
                     /locus_tag="OO357_001788"
     CDS             8639..10267
                     /gene="glpA"
                     /locus_tag="OO357_001788"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit A"
                     /protein_id="HCR5886755.1"
                     /translation="MKTRDSQSSDVIIIGGGATGAGIARDCALRGLRVILVERHDIAT
                     GATGRNHGLLHSGARYAVTDAESARECISENQILKRIARHCVEPTNGLFITLPEDDLS
                     FQATFIRACEEAGISAEAIDPQQARIIEPAVNPALIGAVKVPDGTVDPFRLTAANMLD
                     AKEHGAVILTAHEVTGLIREGATVCGVRVRNHLTGETQALHAPVVVNAAGIWGQHIAE
                     YADLRIRMFPAKGSLLIMDHRINQHVINRCRKPSDADILVPGDTISLIGTTSLRIDYN
                     EIDDNRVTAEEVDILLREGEKLAPVMAKTRILRAYSGVRPLVASDDDPSGRNVSRGIV
                     LLDHAERDGLDGFITITGGKLMTYRLMAEWATDAVCRKLGNTRPCTTADLALPGSQEP
                     AEVTLRKVISLPAPLRGSAVYRHGDRTPAWLSEGRLHRSLVCECEAVTAGEVQYAVEN
                     LNVNSLLDLRRRTRVGMGTCQGELCACRAAGLLQRFNVTTSAQSIEQLSTFLNERWKG
                     VQPIAWGDALRESEFTRWVYQGLCGLEKEQKDAL"
     gene            10257..11516
                     /gene="glpB"
                     /locus_tag="OO357_001789"
     CDS             10257..11516
                     /gene="glpB"
                     /locus_tag="OO357_001789"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase subunit GlpB"
                     /protein_id="HCR5886756.1"
                     /translation="MRFDTVIMGGGLAGLLCGLQLQKHGLRCAIVTRGQSALHFSSGS
                     LDLLSHLPDGQPVADIHSGLESLRQQAPAHPYSLLGPQRVLDLACQAQALIAESGAQL
                     QGSVELAHQRITPLGTLRATWLSSPEVPVWPLPAKKICVVGISGLMDFQAHLAAASLR
                     ELDLSVETAEIELPELDVLRNNATEFRAVNIARFLDNEENWPLLLDALIPVANTCEMI
                     LMPACFGLADDKLWRWLNEKLPCSLMLLPTLPPSVLGIRLQNQLQRQFVRQGGVWMPG
                     DEVKKVTCKNGVVNEIWTRNHADIPLRPRFAVLASGSFFSGGLVAERNGIREPILGLD
                     VLQTATRGEWYKGDFFAPQPWQQFGVTTDETLRPSQAGQTIENLFAIGSVLGGFDPIA
                     QGCGGGVCAVSALHAAQQIAQRAGGQQ"
     gene            11513..12703
                     /gene="glpC"
                     /locus_tag="OO357_001790"
     CDS             11513..12703
                     /gene="glpC"
                     /locus_tag="OO357_001790"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic glycerol-3-phosphate dehydrogenase
                     subunit C"
                     /protein_id="HCR5886757.1"
                     /translation="MNDTSFENCIKCTVCTTACPVSRVNPGYPGPKQAGPDGERLRLK
                     DGALYDEALKYCINCKRCEVACPSDVKIGDIIQRARAKYDTTRPSLRNFVLSHTDLMG
                     SVSTPFAPIVNTATSLKPVRQLLDAALKIDHRRTLPKYSFGTFRRWYRSIAAQQAQYK
                     DQVAFFHGCFVNYNHPQLGKDLIKVLNAMGTGVQLLSKEKCCGVPLIANGFTDKARKQ
                     AITNVESIREAVGVKGIPVIATSSTCTFALRDEYPEVLNVDNKGLRDHIELATRWLWR
                     KLDEGKTLPLKPLPLKVVYHTPCHMEKMGWTLYTLELLRNIPGLELTVLDSQCCGIAG
                     TYGFKKENYPTSQAIGAPLFRQIEESGADLVVTDCETCKWQIEMSTSLRCEHPITLLA
                     QALA"
     gene            12896..13846
                     /locus_tag="OO357_001791"
     CDS             12896..13846
                     /locus_tag="OO357_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
                     /protein_id="HCR5886758.1"
                     /translation="MTESTTSSPHDAVFKTFIFTPETARDFLEIHLPEPLRKLCNLQT
                     LRLEPTSFIEKSLRAYYSDVLWSVETSDGDGYIYCVIEHQSSAEKNMAFRPMRYATAA
                     MQSHLDKGYDRVPLVVPLLFYHGETSPYPYSLNWLDEFDDPQLARQLYTEAFPLVDIT
                     IVPDDEIMQHRRIALLELIQKHIRDHDLIGMVDRITTLLVRGFTNDSQLQTLFNYLLQ
                     CGDTSRFTRFIQEIAERSPLQKERLMTIAERLRQEGHQIGWQEGKLVGLQQGKLEGLQ
                     EGMHEQAIKIALRMLEQGIDRDQVLAATQLSEADLAANNH"
     gene            complement(13887..14357)
                     /locus_tag="OO357_001792"
     CDS             complement(13887..14357)
                     /locus_tag="OO357_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001342598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase"
                     /protein_id="HCR5886759.1"
                     /translation="MSNPPYGERGVGASVARAARWGRIENYMAQVNDSLCLLVQVESK
                     TALDNLDEILDVEGIDGVFIGPADLSASLGYPDNAGHPEVQRIIETSIRRIRAAGKAA
                     GFLAVAPDMAQQCLAWGANFVAVGVDTMLYSDALDQRLAMFKSGKNGPRIKGSY"
     gene            complement(14412..15614)
                     /locus_tag="OO357_001793"
     CDS             complement(14412..15614)
                     /locus_tag="OO357_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide deamidase-related
                     protein YfaY"
                     /protein_id="HCR5886760.1"
                     /translation="MLKVEMLSTGDEVLYGQIVDTNAAWLADFFFHQGLPLSRRNTVG
                     DNLDDLVTILRERSQHADVLIVNGGLGPTSDDLSALAAATAKGEGLVLHEAWLKEMER
                     YFHERGRVMAPSNRKQAELPASAEFINNPVGTACGFAVQLNRCLMFFTPGVPSEFKVM
                     VEHEILPRLRERFSLPQPPVCLRLTTFGRSESDLAQSLDTLQLPPGVTMGYRSSMPII
                     ELKLTGPASEQQAMEKLWLDVKRVAGQSVIFEGTEGLPAQISRELQNRQFSLTLSEQF
                     TGGLLALQLSRAGAPLLACEVIPSQEETLAQTAHWITERRANHFAGLALAVSGFENEH
                     LNFALATPDGTFALRVRFSTTRYSLAIRQEVCAMMALNMLRRWLNGQDIASEHGWIEV
                     VESMTLSV"
     gene            complement(15701..16243)
                     /locus_tag="OO357_001794"
     CDS             complement(15701..16243)
                     /locus_tag="OO357_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708136.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886761.1"
                     /translation="MKKIALAGLAGMLLVSASVNAMSISGQAGKEYTNIGVGFGTEST
                     GLALSGNWTHNDDDGDVAGVGLGLNLPLGPLMATVGGKGVYTNPNYGDEGYAAAVGGG
                     LQWKIGNSFRLFGEYYYSPDSLSSGIQSYEEANAGARYTIMRPVSIEAGYRYLNLSGK
                     DGNRDNAVADGLYVGVNASF"
     gene            16522..16947
                     /gene="nudI"
                     /locus_tag="OO357_001795"
     CDS             16522..16947
                     /gene="nudI"
                     /locus_tag="OO357_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphatase NudI"
                     /protein_id="HCR5886762.1"
                     /translation="MRQRTIVCPLIQNDGAYLLCKMADDRGVFPGQWALSGGGVESGE
                     RIEEALRREIREELGEQLLLTEITPWTFSDDIRTKTYADGRKEEIYMIYLIFDCVSAN
                     REVKINEEFQDYAWVKPEDLVHYDLNVATRKTLRLKGLL"
     gene            complement(16986..17588)
                     /locus_tag="OO357_001796"
     CDS             complement(16986..17588)
                     /locus_tag="OO357_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HCR5886763.1"
                     /translation="MLAFCRSSLKSKKYFIILLALAAIAGLGTHAAWSSNGLPRIDNK
                     TLARLAQQHPVVVLFRHAERCDRSTNQCLSDKTGITVKGTQDARELGNAFSADISDFD
                     LYSSNTVRTIQSATWFSAGKKLTVDKRLLQCGNEIYSAIKDLQSKAPDKNIVIFTHNH
                     CLTYIAKNKRDAIFKPDYLDGLVMHVEKGKVYLDGEFVNH"
     gene            17896..19035
                     /gene="arnB"
                     /locus_tag="OO357_001797"
     CDS             17896..19035
                     /gene="arnB"
                     /locus_tag="OO357_001797"
                     /EC_number="2.6.1.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_024188371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-4-amino-4-deoxy-L-arabinose
                     aminotransferase"
                     /protein_id="HCR5886764.1"
                     /translation="MSEFLPFSRPAMGVEELAAVKEVLESGWITTGPKNQALEQAFCQ
                     LTGNQHAIAVSSATAGMHITLMALEIGKGDEVITPSLTWVSTLNMISLLGATPVMVDV
                     DRDTLMVTPEAIESAITPRTKAIIPVHYAGAPADIDAIRAIGERYGIAVIEDAAHAVG
                     TYYKGRHIGAKGTAIFSFHAIKNITCAEGGLIVTDNENLARQLRMLKFHGLGVDAYDR
                     QTWGRAPQAEVLTPGYKYNLTDINAAIALTQLVKLEHLNTRRREIAQQYQQALAALPF
                     QPLSLPAWPHVHAWHLFIIRVDEQRCGISRDALMEALKERGIGTGLHFRAAHTQKYYR
                     ERFPTLSLPNTEWNSERICSLPLFPDMTTADADRVITALQQLAGQ"
     gene            19039..20007
                     /gene="arnC"
                     /locus_tag="OO357_001798"
     CDS             19039..20007
                     /gene="arnC"
                     /locus_tag="OO357_001798"
                     /EC_number="2.4.2.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate
                     4-deoxy-4-formamido-L-arabinose transferase"
                     /protein_id="HCR5886765.1"
                     /translation="MFEIHPVKKVSVVIPVYNEQESLPELIRRTTTACESLGKEYEIL
                     LIDDGSSDNSAHMLVEASQAENSHIVSILINRNYGQHSAIMAGFSHVTGDLIITLDAD
                     LQNPPEEIPRLVAKADEGYDVVGTVRQNRQDSWFRKTASKMINRLIQRTTGKAMGDYG
                     CMLRAYRRHIVDAMLHCHERSTFIPILANIFARRAIEIPVHHAEREFGESKYSFMRLI
                     NLMYDLVTCLTTTPLRMLSLLGSIIAIGGFSIAVLLVILRLTFGPQWAAEGVFMLFAV
                     LFTFIGAQFIGMGLLGEYIGRIYTDVRARPRYFVQQVIRPSSKENE"
     gene            20007..21988
                     /gene="arnA"
                     /locus_tag="OO357_001799"
                     /pseudo
     CDS             20007..21988
                     /gene="arnA"
                     /locus_tag="OO357_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311170.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-4-amino-4-deoxy-L-arabinose
                     formyltransferase/UDP-glucuronic acid oxidase ArnA"
     gene            21985..22875
                     /locus_tag="OO357_001800"
     CDS             21985..22875
                     /locus_tag="OO357_001800"
                     /EC_number="3.5.1.n3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-deoxy-4-formamido-L-arabinose-
                     phosphoundecaprenol deformylase"
                     /protein_id="HCR5886766.1"
                     /translation="MTKVGLRIDVDAFRGTREGVPRLLEILSKHNIQASIFFSVGPDN
                     MGRHLWRLVKPQFLWKMLRSNAASLYGWDILLAGTAWPGKEIGHANADIIREAAKHHE
                     VGLHAWDHHAWQARSGNWDRQTMIDDIARGLRTLEEIIGQPVTCSAAAGWRADQKVIE
                     AKEAFHLRYNSDCRGAIPFRPLLESGNPGTAQIPVTLPTWDEVIGRDVKAEDFNGWLL
                     NRILRDKGTPVYTIHAEVEGCAYQHNFVDLLKRAAQEGVTFCPLSELLSETLPLGQVV
                     RGNIAGREGWLGCQQIAGSR"
     gene            22875..24527
                     /gene="arnT"
                     /locus_tag="OO357_001801"
     CDS             22875..24527
                     /gene="arnT"
                     /locus_tag="OO357_001801"
                     /EC_number="2.4.2.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A)
                     4-amino-4-deoxy-L-arabinosyltransferase"
                     /protein_id="HCR5886767.1"
                     /translation="MKSVRYLIGIFAFIACYYLLPISTRLLWQPDETRYAEISREMLA
                     SGDWIVPHLLGLRYFEKPIAGYWINSIGQWLFGANNFGVRAGVIFATLLTAALVTWFT
                     LRLWRDKRLALLATVIYLSLFIVYAIGTYAVLDPFIAFWLVAGMCSFWLAMQAQTWKG
                     KSAGFLLLGITCGMGVMTKGFLALAVPVLSVLPWVATQKRWKDLFIYGWLAVISCVLT
                     VLPWGLAIAQREPDFWHYFFWVEHIQRFALDDAQHRAPFWYYVPVIIAGSLPWLGLLP
                     GALYTGWKNRKHSATVYLLSWTIMPLLFFSVAKGKLPTYILSCFAPLAMLLAHYALLA
                     AKNNPLALRINGWINIAFGVTGIIATFVISPWGPMNTPVWQTFESYKVFCAWSIFSLW
                     AFFGWYTLTNVEKTWSFAALCPLGLALLVGFSIPDRVMEGKHPQFFVEMTQESLQPSR
                     YILTDSVGVAAGLAWSLQRDDIIMYRQTGELKYGLNYPDAKGRFVSGDEFANWLNQHR
                     QEGIITLVLSVDRDEDINSLAIPPADAIVRQERLVLIQYRPK"
     gene            24524..24859
                     /gene="arnE"
                     /locus_tag="OO357_001802"
     CDS             24524..24859
                     /gene="arnE"
                     /locus_tag="OO357_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnE"
                     /protein_id="HCR5886768.1"
                     /translation="MIWLTLVFASLLSVAGQLCQKQATCFVAINKRRKHIVLWLGLAL
                     ACLGLAMMLWLLVLQNVPVGIAYPMLSLNFVWVTLAAVKLWHEPVSPRHWCGVAFIIG
                     GIVILGSTV"
     gene            24859..25245
                     /gene="arnF"
                     /locus_tag="OO357_001803"
     CDS             24859..25245
                     /gene="arnF"
                     /locus_tag="OO357_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-amino-4-deoxy-L-arabinose-phosphoundecaprenol
                     flippase subunit ArnF"
                     /protein_id="HCR5886769.1"
                     /translation="MCLIWGLFSVIIASVAQLSLGFAASHLPPMTHLWDFIAALLAFG
                     LDARILLLGLLGYLLSVFCWYKTLHKLALSKAYALLSMSYVLVWIASMVLPGWEGTFS
                     LKALLGVACIMSGLMLIFLPTTKQRY"
     gene            complement(25239..25511)
                     /gene="pmrD"
                     /locus_tag="OO357_001804"
     CDS             complement(25239..25511)
                     /gene="pmrD"
                     /locus_tag="OO357_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal transduction protein PmrD"
                     /protein_id="HCR5886770.1"
                     /translation="MQWNGWHKKSCCNKQDNRHVLMLCDAGGAIKMIAEVKSDFAVKV
                     GDLLSPLQNALYCINREKLHTVKVLSASSYSPDEWERQCTAAGKTQ"
     gene            complement(25616..26971)
                     /gene="menE"
                     /locus_tag="OO357_001805"
     CDS             complement(25616..26971)
                     /gene="menE"
                     /locus_tag="OO357_001805"
                     /EC_number="6.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate--CoA ligase"
                     /protein_id="HCR5886771.1"
                     /translation="MIFSDWPWRHWRQVRGEAIALRLNDEQLNWRELCARVDELATGF
                     AAQGVVEGSGVMLRAWNTPQTLLAWLALLQCGARVLPVNPQLPQPLLEELLPNLTLQF
                     ALVPDGENTFPALTSLHIQLVEGVHAAAWQPTRLCSMTLTSGSTGLPKAAVHTYQAHL
                     ASAQGVLSLIPFGDHDDWLLSLPLFHVSGQGIMWRWLYAGARMTVRDKQPLEQMLAGC
                     THASLVPTQLWRLLVNRSSVSLKAVLLGGAAIPVELTEQAREQGIRCFCGYGLTEFAS
                     TVCAKEADGLADVGLPLPGREVKIVNNEVWLRAASMAEGYWRNGQLVSLVNDEGWYAT
                     RDRGEMHNGKLTIVGRLDNLFFSGGEGIQPEEVERVIAAHPAVLQVFIVSVADKEFGH
                     RPVAVVEYDQQTVDLGEWVKDKLARFQQPVRWLTLPPELKNGGIKISRQALKEWVQRQ
                     Q"
     gene            complement(26968..27930)
                     /gene="menC"
                     /locus_tag="OO357_001806"
     CDS             complement(26968..27930)
                     /gene="menC"
                     /locus_tag="OO357_001806"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HCR5886772.1"
                     /translation="MRSAQVYRWQIPMDAGVVLRDRRLKTRDGLYVCLREGEREGWGE
                     ISPLPGFSQENWEEAQSVLLAWVNNWLAGDCELPQMPSVAFGVSCALAELTDTLPQAA
                     NYRAAPLCNGDPDDLILKLADMPGEKVAKVKVGLYEAVRDGMVVNLLLEAIPDLHLRL
                     DANRAWTPLKGQQFAKYVNPDYRHRIAFLEEPCKTRDDSRAFARETGIAIAWDESLRE
                     PDFAFVAEEGVRAVVIKPTLTGSLEKVREQVQAAHALGLTAVISSSIESSLGLTQLAR
                     IAAWLTPDTIPGLDTLDLMQAQQVRRWPGSPLPLVDADVQEQLL"
     gene            complement(27930..28787)
                     /gene="menB"
                     /locus_tag="OO357_001807"
     CDS             complement(27930..28787)
                     /gene="menB"
                     /locus_tag="OO357_001807"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001641973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HCR5886773.1"
                     /translation="MIYPDEAMPYAPVEWHDCSEGFEDIRYEKSTDGIAKITINRPQV
                     RNAFRPLTVKEMIQALADARYDDNIGVIILTGAGDKAFCSGGDQKVRGDYGGYKDDSG
                     VHHLNVLDFQRQIRTCPKPVVAMVAGYSIGGGHVLHMMCDLTIAADNAIFGQTGPKVG
                     SFDGGWGASYMARIVGQKKAREIWFLCRQYDAKQALDMGLVNTVVPLADLEKETVRWC
                     REMLQNSPMALRCLKAALNADCDGQAGLQELAGNATMLFYMTEEGQEGRNAFNQKRQP
                     DFSKFKRNP"
     gene            complement(28802..29560)
                     /gene="menH"
                     /locus_tag="OO357_001808"
     CDS             complement(28802..29560)
                     /gene="menH"
                     /locus_tag="OO357_001808"
                     /EC_number="4.2.99.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-6-hydroxy-2,
                     4-cyclohexadiene-1-carboxylate synthase"
                     /protein_id="HCR5886774.1"
                     /translation="MILHAQAKHGKLGLPWLVFLHGFSGDCHEWQEVGEAFADYSRLY
                     VDLLGHGGSAAISVDGFDDVTDLLRKTLVSYNILDFWLVGYSLGGRVAMMAACQGLAG
                     LCGVIVEGGHPGLQNAEQRAERQRSDRQWAQRFRTEPLTAVFADWYQQPVFASLNDDQ
                     RRELVALRSNNNGATLAAMLEATSLAVQPDLRANLSARTFAFYYLCGERDSKFRALAA
                     ELAADCHVIPRAGHNAHRENPAGVIASLAQILRF"
     gene            complement(29557..31227)
                     /gene="menD"
                     /locus_tag="OO357_001809"
     CDS             complement(29557..31227)
                     /gene="menD"
                     /locus_tag="OO357_001809"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HCR5886775.1"
                     /translation="MSVSAFNRRWAAVILEALTRHGVRHICIAPGSRSTPLTLAAAEN
                     SAFIHHTHFDERGLGHLALGLAKVSKQPVAVIVTSGTAVANLYPALIEAGLTGEKLIL
                     LTADRPPELIDCGANQAIRQPGMFASHPTHSISLPRPTQDIPARWLVSTIDHALGTLH
                     AGGVHINCPFAEPLYGEMDDTGLSWQQRLGDWWQDDKPWLREAPRLESEKQRDWFFWR
                     QKRGVVVAGRMSAEEGKKVALWAQTLGWPLIGDVLSQTGQPLPCADLWLGNAKATSEL
                     QQAQIVVQLGSSLTGKRLLQWQASCEPEEYWIVDDIEGRLDPAHHRGRRLIANIADWL
                     ELHPAEKRQPWCVEIPRLAEQAMQAVIARRDAFGEAQLAHRISDYLPEQGQLFVGNSL
                     VVRLIDALSQLPAGYPVYSNRGASGIDGLLSTAAGVQRASGKPTLAIVGDLSALYDLN
                     ALALLRQVSAPLVLIVVNNNGGQIFSLLPTPQSERERFYLMPQNVHFEHAAAMFELKY
                     HHPQNWQELETAFADAWRTPTTTVIEMVVNDTDGAQTLQQLLAQVSHL"
     gene            complement(31316..32611)
                     /gene="menF"
                     /locus_tag="OO357_001810"
     CDS             complement(31316..32611)
                     /gene="menF"
                     /locus_tag="OO357_001810"
                     /EC_number="5.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416768.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase MenF"
                     /protein_id="HCR5886776.1"
                     /translation="MQSLTTALENLLRHLSQEIPATPGIRVIDIPFPLKDAFDALSWL
                     ASQQTYPQFYWQQRNGDEEAAVLGAITRFTSLDQAQRFLRQHPEHADLRIWGLNAFDP
                     SQGNLLLPRLEWRRCGGKATLRLTLFSESSLQHDAIQAKEFIATLVSIKPLPGLHLTT
                     TREQHWPDKTGWTQLIELATKTIAEGELDKVVLARATDLHFASPVNAAAMMAASRRLN
                     LNCYHFYMAFDGENAFLGSSPERLWRRRDKALRTEALAGTVANHPDDKQAQQLGEWLM
                     ADDKNQRENMLVVEDICQRLQADTQTLDVLPPQVLRLRKVQHLRRCIWTSLNKADDVI
                     CLHQLLPTAAVAGLPRDLARQFIARHEPFTREWYAGSAGYLSLQQSEFCVSLRSAKIS
                     GNVVRLYAGAGIVRGSDPEQEWQEIDNKAAGLRTLLQME"
     gene            complement(32690..32995)
                     /gene="elaB"
                     /locus_tag="OO357_001811"
     CDS             complement(32690..32995)
                     /gene="elaB"
                     /locus_tag="OO357_001811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein ElaB"
                     /protein_id="HCR5886777.1"
                     /translation="MSNQFGDTRIDDDLTLLSETLEEVLRSSGDPADQKYVELKAHAE
                     KALDDVKKRVSQASDSYYYRAKQAVYRADDYVHEKPWQGIGVGAAVGLVLGLLLARR"
     gene            complement(33050..33511)
                     /locus_tag="OO357_001812"
     CDS             complement(33050..33511)
                     /locus_tag="OO357_001812"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5886778.1"
                     /translation="MIEWQDLHHSELSVSQLYALLQLRCAVFVVEQNCPYQDIDGDDL
                     TGDNRHILGWKNDELVAYARILKSDDDLEPVVIGRVIVSEALRGEKVGQQLMSKTLET
                     CTHHWPDKPVYLGAQAHLQNFYQSFGFIPVTEVYEEDGIPHIGMAREVIQA"
     gene            33576..34493
                     /gene="rbn"
                     /locus_tag="OO357_001813"
     CDS             33576..34493
                     /gene="rbn"
                     /locus_tag="OO357_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease BN"
                     /protein_id="HCR5886779.1"
                     /translation="MELIFLGTSAGVPTRTRNVTAILLNLQHPTQSGLWLFDCGEGTQ
                     HQLLHTAFNPGKLDKIFISHLHGDHLFGLPGLLCSRSMSGIIQPLTIYGPQGIREFVE
                     TALRISGSWTDYPLEIVEIGAGEILDDGLRKVTAYPLEHPLECYGYRIEEHDKPGALN
                     AQALKAAGVPPGPLFQELKAGKTITLEDGRQINGADYLAAPVPGKALAIFGDTGPCDA
                     ALDLAKGVDVMVHEATLDITMEAKANSRGHSSTRQAATLAREAGVGKLIITHVSSRYD
                     DKGCQHLLRECRSIFPATELANDFTVFNV"
BASE COUNT         8435 a         9282 c         8590 g         8276 t
ORIGIN      
        1 gtaatgcctg aaaggctttg cctgctggcg tgacaactcg ctttgatatc atatccctgg
       61 cataaatcaa tggccgccgc gacaccgtta gcagagaaaa gagatgcaat cattgacggg
      121 agtagagata aatactcctt gcgtagaaag ataatccgca tattaatctg accatcattt
      181 tatataagac ggcatattta aacgcattat acaatatggg ttatctttct tttgtaaatt
      241 gtccaacccg atgcaatggc tattttgtta ttatttgatg aataatatca gtgcgtcata
      301 attcaagtta ataaccttaa gggataccag ttatatttaa actaaattaa agtaatgaat
      361 aattttctta taatataagg taaattaaca aaatggcttc gcatttaaca ataaccgaat
      421 agaaaacaac catttcgaca tcaacaatcg cttacattcg cttatatatt gaccacaact
      481 gatacatcag attatgtgat gactcgtgct tagatcaatt tttgcaatca ttagcaaaaa
      541 gattaataaa ccatctatat caatttatcc aacctattct gccgttcaag aaatcgccga
      601 acagttattt ttaacaaatt tttctcttcc cattgacttt cccggacacc ttgtctgacc
      661 taaggtgcgc gaaagccact ttttccttcc tgagttatcc acaaagttat gcacttgcaa
      721 gagggtcatt ttcacactat cttgcagtga atcccaaaca taccccctat atatagtgtt
      781 ctaagcagct tcccttacta caggtagtct gcatgaaact attgcggaaa gaattccaaa
      841 aacaggtacg acatacatga atcagaatct gctggtgaca aagcgcgacg gtagcacaga
      901 gcgcatcaat ctcgacaaaa tccatcgcgt tctggattgg gcggcagaag gactgcataa
      961 cgtttcgatt tcccaggtcg agctgcgctc ccacattcag ttttatgacg gtatcaagac
     1021 ctctgacatc cacgaaacta ttatcaaggc tgccgcagac ctgatctccc gtgatgcgcc
     1081 ggattatcag tatctcgccg cgcgcctggc gatcttccac ctgcgtaaaa aagcctacgg
     1141 ccagtttgag ccgcctgcgc tgtacgacca cgtggtgaaa atggtcgaga tgggcaaata
     1201 cgataatcat ctgctggaag actacacgga agaagagttc aagcagatgg acacgtttat
     1261 cgatcacgac cgtgatatga ccttctctta tgctgccgtt aagcagctgg aaggcaaata
     1321 tctagtacag aaccgcgtga ccggcgaaat ctacgaaagc gcccagttcc tttatattct
     1381 tgttgccgcg tgcctgttct cgaactaccc gcgtgaaacg cgcctgcaat acgtgaagcg
     1441 tttttatgac gcggtttcca catttaaaat ttcgctgccg acgccaatca tgtccggcgt
     1501 gcgtaccccg actcgtcagt tcagctcctg cgtactgatc gagtgcggtg acagcctgga
     1561 ttccatcaac gccacctcca gcgcgattgt gaaatatgtt tcccagcgtg ccgggatcgg
     1621 catcaacgcc ggacgtattc gtgcgctggg cagcccgatt cgcggtggcg aagcgttcca
     1681 taccggctgt atcccgttct acaaacattt ccagacagcg gtgaaatcct gctctcaggg
     1741 cggtgtgcgc ggcggtgcgg caacgctgtt ctacccgatg tggcatctgg aagtggaaag
     1801 cctgctggtg ttgaaaaaca accgtggggt tgaaggcaac cgcgtgcgtc atatggacta
     1861 cggggtacaa atcaacaaac tgatgtatac ccgcctgctg aaaggtgaag atatcaccct
     1921 gttcagcccg tccgacgtac cggggctgta cgacgcgttc ttcgccgatc aggaagagtt
     1981 tgaacgcctg tataccaaat atgagaaaga cgacagcatc cgcaagcagc gtgtgaaagc
     2041 cgttgagctg ttctcgctga tgatgcagga acgtgcgtct accggtcgta tctatattca
     2101 gaacgttgac cactgtaata cccatagtcc gtttgatccg accatcgcgc cagttcgcca
     2161 gtctaacctg tgcctagaaa ttgccctgcc gaccaaaccg ctgaacgacg tcaacgacga
     2221 gaacggtgaa atcgcgctgt gtacgctgtc tgctttcaac ctgggcgcaa ttaatagcct
     2281 ggatgaactg gaagagctgg cgattctggc ggttcgtgca cttgacgcgc tactggatta
     2341 tcaggattac ccgatcccgg ccgccaaacg tggagcgatg gggcgtcgta cgctgggtat
     2401 tggtgtgatt aacttcgctt actatctggc gaagcacggt aaacgctact ccgacggcag
     2461 cgccaacaac ctgacgcata aaaccttcga agcgattcag tattacctgc tgaaagcgtc
     2521 taatgagctg gctaaagagc aaggcgcgtg cccgtggttt aacgaaacca cttacgcgaa
     2581 agggatcctg ccgatcgata cctataagaa agatctggat gccatcgcta atgagccgct
     2641 gcattacgac tgggaagcat tgcgtgaatc aatcaaaacg cacggcctgc gtaactccac
     2701 gctttctgcc ctgatgccgt ccgagacctc ttcgcagatc tctaacgcca ctaacggtat
     2761 tgaaccgccg cgcggttacg tcagcatcaa agcgtcgaaa gacggtattt tgcgccaggt
     2821 ggtgccggac tacgagcacc tgcacgacgc ctatgagctg ctgtgggaaa tgccgggtaa
     2881 cgatggctac ctgcaactgg taggtatcat gcagaaattt atcgatcagt cgatctctgc
     2941 taacaccaac tacgatccgt cacgtttccc gtcaggaaaa gtgccgatgc agcagttgct
     3001 gaaagacctg ctcaccgcct acaaattcgg ggtcaaaacg ctgtattatc agaacacccg
     3061 tgacggcgct gaagacgcgc aggacgatct ggtaccgtca atccaggacg atggctgcga
     3121 aagcggcgca tgtaagatct gatattgaga tgccggatgc ggcgtaaacg ccttatccgg
     3181 cctacggctc ggtttgtagg cctgataaga cgcgccagcg tcgcatcagg ctccgggtgc
     3241 cggatgcagc gtgaacgcct tatccggcct acggctcgga tttgtaggcc tgataagacg
     3301 cgccagcgtc gcatcaggca caggatgcgg cgtaaaatgc cttatccggc attaaactcc
     3361 caacaggaca cactcatggc atataccacc ttttcacaga cgaaaaatga tcagctcaaa
     3421 gaaccgatgt tctttggtca gccggtcaac gtggctcgct acgatcagca aaaatatgac
     3481 atcttcgaaa agctgatcga aaagcagctc tctttcttct ggcgtccgga agaagttgac
     3541 gtctcccgcg accgtataga ttaccaggcg ctgccggagc acgaaaaaca catctttatc
     3601 agcaacctga aatatcagac gctgctggat tccattcagg gtcgtagccc gaacgtggcg
     3661 ctattgccgc ttatttctat tccggaactg gaaacctggg tcgaaacctg ggcgttctca
     3721 gaaacgattc attcccgttc ctatactcat atcattcgta atatcgttaa cgatccgtct
     3781 gttgtgtttg acgatatcgt caccaacgag cagatccaga aacgtgcgga agggatctcc
     3841 agctattacg atgaactgat cgaaatgacc agctactggc accttctggg cgaaggcacc
     3901 cacaccgtta acggtaaaac cgtaaccgtt agcctgcgcg agctgaagaa aaaactgtat
     3961 ctctgcctga tgagcgttaa cgcgctggaa gcgatccgct tctacgtcag ctttgcttgt
     4021 tcctttgcct ttgcagaacg cgaactgatg gaaggtaacg ccaagattat tcgcctgatt
     4081 gcccgcgatg aagccctgca cctgaccggt actcagcata tgctgaatct gctgcgcagc
     4141 ggcgcggatg atccggagat ggcggaaatt gccgaagaat gtaagcagga gtgctatgac
     4201 ctgtttgtcc aggcggcaca gcaagagaaa gactgggcgg attatctgtt ccgtgacggt
     4261 tcgatgattg gtctgaataa agatattctc tgccagtacg ttgaatacat caccaatatc
     4321 cgcatgcagg cagtcggttt ggatctgccg ttccagacgc gctccaaccc gatcccgtgg
     4381 atcaacacct ggctggtgtc tgataacgtg caggttgctc cgcaggaagt ggaagtcagt
     4441 tcttatctgg tcgggcagat tgacgcggaa gtggacaccg acgatttgag taacttccag
     4501 ctctgatggc ccgcgttacc ctgcgcatca ctggcacaca actgctgtgc caggatgaac
     4561 acccttccct tctggcggcg ctggaatccc acaatgtggc ggttgagtac cagtgccgcg
     4621 aaggttactg cggctcctgt cgcacccgac tggtcgcagg tcaggtggac tggattgccg
     4681 aaccgttagc ctttattcag ccgggggaaa ttttgccctg ttgttgccgg gcaaaaggcg
     4741 atattgaaat cgagttgtga tcgggtacta gcgccagata cgacgcttac gcgtattatc
     4801 tggcctacaa cgattacatg gcgtagtaat acgctttcac ctgctcccag tccgctttag
     4861 ggatcggctc cagatatttt tccagctggc ggaagtcatg attaatcgct ttatcgcgac
     4921 gcaagcggcg acgacttttc tccagatcaa gaaaaccagc ttctgcctta ccttctgttt
     4981 tcacatagat atggcgaaca tagcaacagc catgctgacg attaacgcta tgcattttct
     5041 taaacgccag tgccactgct ttcaacatgg cttgccgtac ttcgtcagaa taaggcgata
     5101 ctgcatgccg ggcataccag tcagcaatgc tgatgaaccc cgccatatct tcagtcacca
     5161 gcaacgcgcg ccattcaccc tcaattttca ccgcttcacc aaaaacgatc ttcggcacga
     5221 tgacaccggc ccgttccagt tctttaatta ccgcaacctc acggacaatc gttggtcggc
     5281 cgaacggata acgtactgaa tgaaacagat gatgcgtcat gcgctttaca tacagctttt
     5341 tgccgttgcg ctcgacgcat tgcaccccgc tcataccatt acggcgatag ttaggctctt
     5401 caacccagtc gccctctgtt gcccaccagt gattaaattc gtcgtacttt gctgaaactg
     5461 ccatacccta tcgcctgtca tttttattaa cgacaatgac tataggtggt tacctgagga
     5521 aaatcttaat gaaacgtgtc gtattaatga atttcttttg ccgctacagg tgtgacaccg
     5581 cttttgccct tcatttgcag cgcatagatt ccaccagcca ctaccactaa tagtgcgccc
     5641 agcagataaa atgtgaattt aatcagtcgg atacagcgat gaattttatt cgacagggat
     5701 taggcatcgc cttacaacca gagttaacgc tgaaaagcat tgcaggtgaa ttgtgttccg
     5761 ttcctcacga accaaccttc tatcgacaga tttcgttgct gactaaagaa aagccggtag
     5821 aaggcagtcc actgttttta ctacaaatgt gcatggaaca attagtggcg attggaaaaa
     5881 tttgatatga gacaggatgg cgcatgaacg ccatcctgca gtaacattac tctttattaa
     5941 gaaattttac tgccttatca gggaaatcag taaacagccc atttacaccc gctttgttat
     6001 acagagcatc atataactga ttcacatcag gagtgtattc aggcagttta tctgaccgca
     6061 cggtataagg atgcactacc agtttattct gctgggcatc ttgcaccatg ccagtgagtt
     6121 tgatattacc cggctgcgat gtctcctcaa tcaacatatg gtaatccgga ccaataccat
     6181 ctgcatattc cgccacctgt ttcatggctc ccggcttaaa catccagtcg tagttgtaat
     6241 taacccagct tccatccggc tgtttctgct gcgtttcatt ccagtcggta taggcaatca
     6301 gctgtaccag attgagctcc atgcccattt tgggttccag ctcattctta atacgcttca
     6361 gctcatcagc atcaaaacat tgcaaataaa ctttatcgtc tttaccggtg taaccatatt
     6421 tcttcagcac ttccagcgtt tttgccgcaa tatccttccc ttcctgatga tggaaccacg
     6481 gcgctttgat ttctggatag ataccgatat ttttcccggt agagtgattt aacccctgaa
     6541 caaattcaat ctcttcttca aaggtgtgca cccggaagtc ggacttaccc attgggaaac
     6601 gccccggata agtctgtact tttttaccgt tttcaatatc gaaaccttcg gtaaatttca
     6661 gcgacttaat ttcatccagc gtgaaatcta tcgcgtagta acgaccgtct ttgcgcgccc
     6721 gatccgggaa acgatcggca acatcagtaa cacgatcgag ataatggtca tgcagaacaa
     6781 ccagatggtc gtctttggtc atcaccaaat cctgttccag ataatccgct ccctgcgcat
     6841 acgccatcgc ttttgctggc agcgtatgct ccggcaaata tccactggca ccgcgatggg
     6901 cgatgactat tttttcgttg ctgtccgccg ccattgcact gcttcccatg actatagtgc
     6961 tcatcatgat cgccatgcta aggtttttca gcttcaattt catgccatta gcctccgttg
     7021 cgtttttgca gtaattgttc atggcgacgt ttttcgccaa tcatcacaac aatcaacaag
     7081 ataaccgcca gaatgctgcc gccaatcatt accataaagc cgccatccca gccgaagaag
     7141 tccacggtgt agccaacaat cgcgctcgcc gccaccgaac cgcccaggta accaaacagc
     7201 ccggtaaagc ccgctgccgt acctgccgct tttttcggtg ccagttccag cgcatgcaga
     7261 ccgatcagca tcacaggacc gtagatcagg aagccgataa caatcataca aatcatatcg
     7321 acggttgggt tacctgccgg gttcatccag taaacgatag tcgcgatggt caccagtgtc
     7381 ataaagaaga cgccggttgc cccacggtta ccacggaaga ctttgtccga catccagccg
     7441 cacagcaggg tgcccggaat acccgcatac tcatagaaga agtaggccca ggaggattta
     7501 tccagcgcga aatgcttaac ctctttcaga taagtcggtg accagtcgag gatgccgtaa
     7561 cgcagcagat aaacgaacac gttggcgatg gcgatgtacc acagcagttt gttcggcagc
     7621 acgtactgca tgaagatttg cttcgcggtc agctcctgtt ccgctttttc gttatagtcg
     7681 tccggataat catttttgta ctcttcgatc ggcggcaagc cacaggattg cggggtatcg
     7741 cgcatcatcg caaaggcgaa taatgccacc agaatggcgc agaaagcagg catatagagc
     7801 gccgcatgcc agtcattgaa ccaggccatc cccagcagga acagcagcgg cggaatacca
     7861 ccaccgacgt tgtgcgcaca gttccacact gacacaatgc cgccacgttc tttctgcgac
     7921 caccagtgca ccatagtacg accacacggc ggccacccca tcccctggaa ccaaccgcag
     7981 aggaacaaca gtacaaacat caccgcaatg ctcgacgtcg cccatggcac aaagcccata
     8041 aacaacatca ctgccgccgc cagaatcaaa cctgcgggca ggaaaacgcg cggattcgag
     8101 cgatccgata ccgaacccat gatgaatttc gaaaatccat aagcaatcga gatccccgaa
     8161 agggcaaaac ctaaatcacc gcgtgagaat ccctgctcaa ccagataagg catagcaagc
     8221 gcaaagttct tacgaaccaa atagtaagcc gcatagccaa agaatatccc caggaaaatt
     8281 tgccagcgca atcgacgata agtcggatcg atctccgcgg caggtaagcg cgctttgtgt
     8341 ggcgctggtt taaaaatact caacattgat agcctccgtg gcccgtggtc ttatttatga
     8401 ttaacagcct gattcagtga gagaacctgc cgtttcttga gttgccgcga tgttaagaaa
     8461 acattcataa attaaatgtg aattgccgca cacattatta aataagattt acaaaatgtt
     8521 caaaatgacg catgaaatca cgttttactt tcgaatcatg agcgaatatg cgcgaaatca
     8581 aacaattcat gtttttacta tggctaaatg gtaaaaaacg aacttcagag ggataacaat
     8641 gaaaactcgc gactcgcaat caagtgacgt gattatcatt ggcggcggcg caacgggagc
     8701 cgggattgcc cgcgactgtg ccctgcgcgg gctgcgcgtg attttggttg agcgccacga
     8761 catcgcaacc ggtgccaccg ggcgtaacca cggcctgctg cacagcggtg cgcgctatgc
     8821 agtaaccgat gcggaatcgg cccgcgaatg cattagtgaa aaccagatcc tgaaacgcat
     8881 tgcacgtcac tgcgttgaac caaccaacgg cctgtttatc accctgccgg aagatgacct
     8941 ctcattccag gccactttta ttcgcgcctg cgaagaagca gggatcagcg cagaagctat
     9001 agacccgcag caagcgcgca ttatcgaacc tgccgttaac ccggcactga ttggcgcggt
     9061 gaaagttccg gatggcaccg ttgatccatt tcgtctgacc gcagcaaaca tgctggatgc
     9121 caaagaacac ggtgccgtta tccttaccgc tcatgaagtc acggggctga ttcgtgaagg
     9181 cgcgacggtg tgcggtgttc gtgtacgtaa ccatctcacc ggcgaaactc aggcccttca
     9241 tgcacctgtc gtggttaatg ccgctgggat ctgggggcaa cacattgccg aatatgccga
     9301 tctgcgcatt cgcatgttcc cggcgaaagg atcgctgctg atcatggatc accgcattaa
     9361 ccagcatgtg atcaaccgct gccgtaaacc ttccgacgcc gatattctgg tgcctggcga
     9421 taccatttcg ctgattggta ccacctcttt acgtattgat tacaacgaga ttgacgataa
     9481 tcgagtgacg gcagaagagg ttgatattct gctgcgtgaa ggggaaaaac tggcccccgt
     9541 gatggcgaaa acgcgcattt tgcgggccta ttctggcgtg cgcccgctgg ttgccagcga
     9601 tgacgacccg agcggacgta acgtcagccg tggcatcgtg ctgctcgacc atgctgaacg
     9661 cgatggtctg gacggattta tcaccatcac cggtggcaaa ctgatgacct atcggctgat
     9721 ggctgaatgg gctaccgacg cggtatgccg caaactgggc aacacgcgcc cctgtacgac
     9781 tgccgatctg gcactgcctg gttcacaaga acccgctgaa gttaccttgc gtaaagtcat
     9841 ctccctgcct gccccgctgc gcggttctgc ggtttatcgt catggcgatc gcacgcctgc
     9901 ctggctgagc gaaggccgtc tacaccgtag cctggtatgt gagtgcgaag cggtaactgc
     9961 gggtgaagtg cagtacgcgg tagaaaattt aaacgttaat agcctgctgg atttacgccg
    10021 tcgtacccgt gtggggatgg gcacctgcca gggcgaactc tgcgcctgcc gcgctgccgg
    10081 actgctgcaa cggtttaacg tcacgacgtc cgcgcaatct atcgagcaac tttccacctt
    10141 ccttaacgaa cgctggaaag gcgtgcaacc catcgcctgg ggagacgcac tgcgcgaaag
    10201 cgaatttacc cgctgggttt atcagggact gtgtggtctg gagaaggagc agaaagatgc
    10261 gctttgatac tgtcattatg ggcggcggcc tcgccggatt actctgtggc ctgcaactgc
    10321 aaaaacacgg cctgcgctgt gccattgtca cgcgtggtca aagcgcgttg catttctcat
    10381 ccggttcgct ggatttgctg agccatctgc cagatggtca acccgtggca gacattcaca
    10441 gtggactgga atctttgcgt cagcaggcac ctgcacatcc ttactctctt ctcgggccac
    10501 aacgcgtgct cgatctcgct tgccaggcgc aggcattaat cgctgaaagc ggtgcgcaat
    10561 tgcagggcag cgtagaactg gctcaccagc ggattacgcc gctcggcact ttgcgtgcaa
    10621 cctggttaag ttcgccagaa gttcccgtct ggccgctacc cgcgaagaag atatgtgtag
    10681 tggggataag cgggctgatg gatttccagg cgcatcttgc ggcggcttcg ttgcgcgaac
    10741 tggatctttc ggttgaaacc gcagaaatag agctgccgga actggatgtc ctgcgcaata
    10801 acgccaccga atttcgcgcg gtgaatatcg cccgtttcct tgataatgaa gaaaactggc
    10861 cgctgttact tgatgcgctt attcctgtcg ccaatacctg cgaaatgatc ctgatgcccg
    10921 cctgcttcgg cctggccgat gacaaactgt ggcgttggtt gaatgaaaaa ctaccttgtt
    10981 cactgatgct tttgccaacg ctgccgcctt ccgtgctggg cattcgtctg caaaaccagt
    11041 tacagcgcca gtttgtgcgc caaggtggag tgtggatgcc gggcgatgaa gtgaaaaaag
    11101 tgacctgtaa aaatggcgta gtgaatgaaa tctggacccg caatcacgcc gatattccgc
    11161 tacgtccacg tttcgcggtt ctcgccagcg gcagtttctt tagtggcgga ctagtagcgg
    11221 aacgtaacgg cattcgcgag ccgattctcg gccttgatgt gctacaaacc gccacgcggg
    11281 gtgaatggta taagggcgat ttttttgcgc cgcaaccgtg gcagcagttc ggtgtaacca
    11341 ctgatgagac gctacgcccg tcgcaggcag ggcaaaccat tgagaacctg tttgccatcg
    11401 gttcggtgct gggcggattt gatcccatcg cccagggatg cggcggcggt gtttgtgccg
    11461 tcagtgcttt acatgccgct caacagattg cccaacgcgc aggaggccaa caatgaacga
    11521 caccagcttc gaaaactgca ttaagtgcac cgtctgcacc accgcctgcc cggtgagccg
    11581 ggtgaatccc ggttatccag ggccaaaaca agccgggccg gatggcgagc gtctgcgttt
    11641 gaaagatggc gcactgtatg acgaggcgct gaaatattgc atcaactgca aacgttgtga
    11701 agtcgcctgc ccgtccgatg tgaagattgg cgatattatc cagcgcgcgc gggcgaaata
    11761 tgacaccacg cgcccgtcgc tgcgtaattt tgtgttgagt cataccgacc tgatgggtag
    11821 cgtttccacg ccgttcgcac caatcgtcaa caccgctacc tcgctgaaac cggtgcggca
    11881 gctgcttgat gcggcgttaa aaatcgatca tcgtcgcacg ctaccgaaat actccttcgg
    11941 cacgttccgt cgctggtatc gcagcatagc ggctcagcaa gcacaatata aagaccaggt
    12001 cgctttcttt cacggctgct tcgttaacta caaccatccg cagttaggta aagatttaat
    12061 taaagtgctc aacgcaatgg gtaccggtgt acaactgctc agcaaagaaa aatgctgcgg
    12121 cgtaccgcta atcgccaacg gctttaccga taaagcacgc aaacaggcaa ttacgaatgt
    12181 agagtcgatc cgcgaagctg tgggagtaaa aggcattccg gtgattgcca cctcctcaac
    12241 ctgtacattt gccctgcgcg acgaataccc ggaagtgctg aatgtcgaca acaaaggctt
    12301 gcgcgatcat atcgaactgg caacccgctg gctgtggcgc aagctggacg aaggcaaaac
    12361 gttaccgctg aaaccgctgc cgctgaaagt ggtttatcac actccgtgcc atatggaaaa
    12421 aatgggctgg acgctctaca ccctggagct gttgcgtaac atcccggggc ttgagttaac
    12481 ggtgctggat tcccagtgct gcggtattgc gggtacttac ggtttcaaaa aagagaacta
    12541 ccccacctca caagccatcg gcgcaccact gttccgccag atagaagaaa gcggcgcaga
    12601 tctggtggtc accgactgcg aaacctgtaa atggcagata gagatgtcca caagtcttcg
    12661 ctgcgaacat ccgattacgc tactggccca ggcgctggct taaactccct tctgatgccc
    12721 ggtaagcatg tggttaccgg gcatttttgc gtacacgatt ccgtgcccaa tgtatgcgtt
    12781 gcaacgcagt gaaaattcct ctgaaaacgt ctcgcaaagg ctgaaactgg cagatgtcaa
    12841 aggcctggga taaccgtaat gtcgcgtcat cataaatatc aggtgacgga caaccatgac
    12901 cgaatcaaca acctcctccc cgcatgatgc ggtatttaaa acctttatat tcacacccga
    12961 aaccgcacgg gattttctcg aaatacattt accagaacca ctgcgcaagc tttgcaacct
    13021 gcaaacctta cgcctggaac ccactagttt tattgaaaaa agtttacgcg cttactactc
    13081 ggatgttttg tggtccgtgg aaaccagcga cggtgacggc tatatctact gcgtgattga
    13141 acatcaaagc tctgcagaaa agaatatggc ttttcggcca atgcgctatg ccactgccgc
    13201 catgcagagt cacctagata aaggctatga cagagttccg ctggtggtgc cattgctgtt
    13261 ttatcatggc gaaacatcgc cctacccgta ctcacttaac tggctggatg agtttgacga
    13321 tccgcaactt gcccggcagt tgtacaccga agcttttccg ttggtggata tcaccatcgt
    13381 acctgacgat gagatcatgc aacatcggcg tatagctctg ctggaactga ttcaaaagca
    13441 tattcgcgac cacgatttaa tcggcatggt cgacaggatc accacgcttt tggttagagg
    13501 cttcactaat gacagccagc tacaaacact gtttaattat ctgctgcaat gcggcgatac
    13561 ctcccgtttc acccgtttta ttcaggagat tgccgaacgt tcaccactac aaaaggagag
    13621 attaatgact attgctgaac ggctacggca ggaaggacat caaattggct ggcaagaagg
    13681 taaattagta ggtttgcagc aaggtaaatt agaaggtttg caggaaggta tgcatgaaca
    13741 agccattaaa attgctttgc gcatgctgga acagggcatt gatcgtgacc aagtgctcgc
    13801 ggccacccag ctaagcgaag ccgatctggc agcgaataac cactaattaa cacaggccca
    13861 cagccgatcc ccatgggcct ttgatatcaa taactacctt ttatgcgtgg cccatttttg
    13921 cctgatttaa acatcgccag tcgttgatcc agggcatcgc tgtagagcat cgtgtcaacg
    13981 ccaacagcga caaagttcgc tccccacgcc aggcattgct gcgccatatc aggagccaca
    14041 gccagaaaac cagccgcttt acccgcagca cggatccgcc gaatgctggt ttcaataatt
    14101 cgctgcactt ccgggtgccc ggcgttatcc gggtagccca acgacgcaga aagatccgct
    14161 ggtccaataa acacgccatc aatcccttcg acgtcgagga tctcgtccag gttatccagt
    14221 gccgttttac tttccacctg cactaacaga caaagcgaat cgttgacttg cgccatgtaa
    14281 ttctcaatgc gtccccagcg cgcagcccgt gccacactgg ccccaacacc acgctcaccg
    14341 tagggagggt tggacaaatt ttgccggagg gggaatgtgc cggtgcagta aaccggcaca
    14401 gagtggcgaa atcagacgga taaagtcatg gactcaacaa cctcaatcca gccatgctca
    14461 ctagcgatgt cctggccgtt taaccaacgg cgtagcatat tcagtgccat cattgcgcac
    14521 acttcctgac ggatagccag gctgtagcgt gtagtgctga aacgcacacg cagagcgaaa
    14581 gtgccgtctg gcgtggctag cgcaaagttg agatgctcgt tctcgaaacc cgaaacagcc
    14641 agtgccagcc cggcaaaatg gttggcccgc cgttctgtaa tccagtgcgc agtttgcgcc
    14701 agggtttctt cctgtgaagg aatcacttca cacgccagca atggagcacc tgcgcgagaa
    14761 agttgcaaag ccaataaacc accggtgaat tgctcgctca acgtcaggct gaactggcgg
    14821 ttttgcaatt cgcgactgat ctgcgcgggc agtccttcgg tgccttcgaa aatcacgctc
    14881 tgtccggcaa cacgtttaac gtccagccac agtttttcca tcgcctgttg ctcgcttgcc
    14941 ggtccggtga gtttcagttc gatgatcggc attgaggagc gatagcccat tgttacgccc
    15001 ggcggcagtt gtagagtgtc caggctttgt gccagatcgc tttccgaacg accaaaggta
    15061 gttaaacgca gacaaaccgg cggctgtggt aaagaaaaac gctcgcgcag gcgcggcagg
    15121 atttcgtgct cgaccatcac cttaaattct gacggtacgc cgggagtaaa gaacatcagg
    15181 caacgattaa gctgcacggc aaaaccacag gcggtgccta ccgggttatt gataaattca
    15241 gcactggcag gcagctccgc ttgtttacgg ttgctcggtg ccattactcg tccacgttcg
    15301 tggaaatagc gttccatctc tttgagccag gcttcatgca gcaccaggcc ttcacctttt
    15361 gctgtcgcag cggcgagtgc gcttaaatca tcgctggtcg gtcccagccc gccgttaacg
    15421 atcagcacat cggcgtgctg actacgttcg cgaagaatgg tgactaagtc atcaaggtta
    15481 tcccccaccg tattgcggcg agataatggc agcccctgat gaaagaaaaa atcggccagc
    15541 caggcagcgt tagtgtcaac gatttgcccg tataacactt catccccggt ggatagcatt
    15601 tccactttta acatggttat ctcctgcttt tgttatcgaa tcactataac gcaaagggag
    15661 ataaggcgta gtaagaaact gaatgccgcg ccggaaggga tcagaaactg gcgttaaccc
    15721 caacatacag gccgtcagcc acagcgttgt cgcggttacc gtctttaccc gacagattca
    15781 ggtagcgata acccgcctca atactgactg gacgcataat ggtgtaacgc gcgccagcat
    15841 tcgcttcctc ataactttga ataccgctgg agagcgaatc cggagagtag taatactcgc
    15901 caaacaaacg gaagctgttg ccaattttcc actgcaaacc acctcctacc gctgcggcat
    15961 aaccttcatc gccgtaattc gggttggtgt acacgccttt tccgccaacg gtcgccatta
    16021 acggcccgag aggcagattc aaccccagcc ccacgcccgc gacgtcaccg tcgtcgtcgt
    16081 tatgtgtcca gttaccgctt aaagccaggc ccgtcgattc agtcccaaaa ccgacaccaa
    16141 tattggtgta ttctttaccc gcctggccgc tgatgctcat tgcattgacc gatgcagaaa
    16201 ccaacagcat tccggccaga cctgctagcg caattttttt cattatcccc tttcccacaa
    16261 gcaattaatc aaaattccca aagctgcggg attgtactgc ctggcacaga ggattcaatg
    16321 agcgaaaaaa gggatctagt cacttattgt aactaaatac ttcttaaata attaaacgcc
    16381 gaaaaaataa acacacttta ccgatacaca caaaaataat aataagtgca gttatcattg
    16441 caaaagagat tactgtcact ttcacatatt tctgagttac cgtattctca tggtttcttt
    16501 ttatttataa ggaatacccc tgtgcgacaa cggactattg tatgcccttt gattcaaaat
    16561 gatggtgctt atttgctgtg caaaatggcc gacgatcgcg gcgttttccc cggtcaatgg
    16621 gcgctttcgg gtggcggcgt ggagtctggc gaacgaattg aagaggcact acgccgcgaa
    16681 attcgcgaag aactgggaga acagctgctt ttgacagaaa tcacgccgtg gaccttcagc
    16741 gatgatattc gcaccaagac atatgcagat ggtcgcaagg aagagattta tatgatttac
    16801 ctgatttttg actgcgtttc tgccaaccga gaagtgaaaa taaacgaaga gtttcaggac
    16861 tacgcgtggg taaaacctga agatctggtg cattatgatt taaatgttgc cacccgaaaa
    16921 acgttacgtt tgaaaggtct tctttaacaa tgatgacagc catcactggc tgtcagattg
    16981 aaaatttagt ggttaacgaa ttccccatcc agataaactt tgcctttttc cacatgcatg
    17041 actaaaccat ccagataatc aggtttaaat atcgcatcac gcttattttt agcaatatat
    17101 gtcaggcaat gattatgggt gaaaataacg atatttttat caggcgcttt gctttgtaag
    17161 tccttaattg cactataaat ctcattaccg cactgaagaa gtcgtttatc taccgtcagc
    17221 tttttacccg cggaaaacca ggtcgccgac tgaatggtcc ggacggtatt actggaataa
    17281 agatcgaaat cagagatgtc agcgctaaaa gcgttgccca gttcacgggc atcctgggta
    17341 cctttaaccg taatacctgt tttatctgac aagcattgat tggttgaacg atcgcaacgt
    17401 tcagcatggc gaaacaaaac gacaaccggg tgctgctgtg ccagtctggc caatgtttta
    17461 ttgtcgatac gtggcaaacc attgctactc caggcggcat gagtacccag tccggcaatt
    17521 gcagcgagcg ccagtaaaat gatgaaatat ttttttgact tcaatgatga gcggcaaaaa
    17581 gctaacacgt aaactccacc tatagacaag cgcaaccaga caattaccgt gaaattgagc
    17641 gacatttctg gcgataatta gcagttggtg taatattaaa aatcctacga tgtcggcaaa
    17701 atgcctcaaa tttttgccaa atgcaaagcc taaataagaa aaaatataaa aatttcaata
    17761 tttacgtcta atattagttt cttaaggtta agttaatatt ctatccttaa aatttcgctc
    17821 caaatggcaa aatatacaca acactcttta tagcaaatat aagtggacag gtattcaatg
    17881 gcggaaggaa aagcaatgtc agaatttttg cctttttcgc gaccagcaat gggcgtggag
    17941 gaactcgctg cagttaaaga ggttctcgaa tccggttgga tcacaaccgg tccgaagaat
    18001 caggcgcttg agcaagcctt ttgccagttg acgggaaatc agcatgccat cgcggtcagt
    18061 tcagccaccg ccggaatgca tatcacgcta atggcgttgg aaattggcaa gggcgatgaa
    18121 gtgattacgc cttccctgac ctgggtttca accctcaata tgatttcctt gctgggtgca
    18181 acgccggtaa tggtggatgt cgaccgcgat acgctgatgg tcacgcctga agcgatcgaa
    18241 tcagccatta cgccacgcac taaagccatc attccggtgc attatgccgg tgcgccagca
    18301 gatattgacg ccattcgcgc cattggcgaa cgttacggca tcgcagttat cgaagatgct
    18361 gcccatgccg tcggtacgta ttacaaaggg cgacatattg gcgcaaaagg taccgctatt
    18421 ttttcatttc atgccattaa aaatattacc tgtgctgaag gtggcctgat tgtaactgat
    18481 aatgaaaacc ttgcccgcca gctacggatg ctgaaatttc acggtctggg tgtcgatgcc
    18541 tatgacagac aaacctgggg ccgtgcaccg caggctgaag tcttaacacc gggctataag
    18601 tacaatctga ccgatattaa cgccgcgatt gccctgacac agttagtcaa attagagcac
    18661 ctcaacaccc gtcggcgcga aattgcccag caatatcagc aagcactggc agctctcccc
    18721 tttcagccat taagccttcc cgcctggccg cacgttcacg cctggcatct gtttattatt
    18781 cgtgtcgatg aacaacgttg tggtatcagt cgcgatgcgt tgatggaagc gttaaaagaa
    18841 agaggcattg gtaccgggtt acatttccgc gccgctcaca cacaaaaata ttatcgcgag
    18901 cgttttccca cgctgtcgtt accgaatacc gaatggaata gcgaacgcat ctgttcgttg
    18961 ccgctgttcc cggatatgac taccgccgat gccgaccgcg tcatcacagc ccttcagcaa
    19021 ctcgcaggac aataagccat gtttgaaatc caccctgtta agaaagtctc ggtggttatt
    19081 cccgtttata acgagcagga aagcttaccg gaattaatca ggcgcaccac cacagcctgt
    19141 gaatcgttgg ggaaagagta tgagatcctg ctgattgatg acggcagtag cgataattcc
    19201 gcgcatatgc tggtcgaagc ctcacaagcg gagaacagcc atattgtgtc tattttgatt
    19261 aaccgcaatt acgggcaaca ttcagcgatt atggcgggat tcagtcacgt tactggcgac
    19321 ttaattatta cccttgatgc cgatctccag aatccgccag aagaaatccc ccgcctggtg
    19381 gcaaaagccg atgaaggtta cgacgtggta gggactgtac gccagaaccg ccaggacagc
    19441 tggtttcgta aaacagcttc gaagatgatt aaccggctta ttcagcgcac cactggcaaa
    19501 gcgatgggtg actacggttg tatgctgcgc gcctatcgcc gtcatattgt cgatgcgatg
    19561 ttgcactgcc atgaacgcag cacctttatc ccgattctgg cgaatatctt cgcccgccgt
    19621 gcaattgaaa ttccagtaca tcatgccgag cgtgagtttg gtgaatccaa atacagtttt
    19681 atgcgcctga ttaatttgat gtacgacctg gtgacctgcc ttaccacaac gccgctacgt
    19741 atgctgagtc tgctcggcag cattattgcg attggaggtt ttagcattgc ggtgctgctg
    19801 gtgattttac gcctgacctt cggaccacaa tgggcggcag aaggcgtctt tatgctattt
    19861 gccgtgctgt ttacttttat tggcgctcag tttatcggca tgggattact cggtgaatat
    19921 atcggcagga tctacaccga tgtccgcgcc cgcccccgct attttgttca gcaagttatc
    19981 cgtccatcca gcaaggaaaa tgaataatga aaaccgtcgt ttttgcctac cacgatatgg
    20041 gatgcctcgg tattgaagct ctgctggctg ccggttacga aattagcgcc atttttaccc
    20101 ataccgataa tcccggtgaa aaagcctttt atggttcggt ggctcatctg gcggcggaaa
    20161 gagacattcc ggtttatgcg ccggataacg ttaatcatcc gctgtgggtg gaacgcattg
    20221 cccaactgtc accagaggtg attttctctt tttattatcg ccatcttatt tgcgacgaga
    20281 tttttcagct cgctcccgca ggtgcattta atctgcacgg ttcgctgtta ccaaaatatc
    20341 gtggtcgcgc gccgctgaac tgggtgctgg tgaacggtga aacggaaacg ggcgttacat
    20401 tgcaccgaat ggtaaacgtg ccgatgctgg ggccattgtg gcgcaactgc gcgttgccat
    20461 tgcgccagac gatatcgcta ttacgctgca tcataaattg tgccatgccg cgcgccagct
    20521 actggaacag acattacccg ccattaaaca cggtaatatt ctggaaatcg cccagcgcga
    20581 aaacgaagcc acctgttttg gtcgcagaac gccggatgac agtttccttg aatggcataa
    20641 accggcatcc gtactgcaca acatggtacg tgccgttgcc gatccgtggc cgggtgcctt
    20701 cagctatgtt ggcaatcaga aattcaccgt ctggtcgtcg cgtgttcatc ctcatgccag
    20761 caaagcacag ccggggagcg tgatttctgt tgcgccactg ctgattgcct gtggcgatgg
    20821 cgcgctggaa atcgtcaccg gacaggcggg cgacggcatt actatgcagg gctcgcaatt
    20881 agcgcagatg ctgggcctgg tgcaaggttc acgcttgaat agccagcctg cctgcaccgc
    20941 ccgacgccgt acccgggtac tcatcctcgg ggtgaatggc tttattggca accatctgac
    21001 agaacgcctg ctgcgcgaag atcattatga agtttacggt ctggatattg gcagcgatgc
    21061 gataagccgt tttctgaatc atccacattt tcactttgtc gaaggcgata tcagtattca
    21121 ttccgaatgg attgagtatc acgtcaaaaa atgtgatgtc gtcttgccgc tggtggcgat
    21181 agccacgccg attgaatata cccgcaaccc gctgcgcgta tttgaactcg attttgaaga
    21241 gaatctgcgc attatccgct actgcgttaa gtaccgtaag cgaatcatct tcccgtcaac
    21301 ttcagaagtt tatgggatgt gtagcgataa atacttcgat gaggaccatt ctaatttaat
    21361 cgtcggcccg gtgaataaac cacgctggat ttattcggtg tcaaaacaat tacttgatcg
    21421 ggtgatctgg gcctatggcg aaaaagaggg tttacagttc accctcttcc tcccgtttaa
    21481 ctggatggga ccacgactgg ataaccttaa tgcagcgcga attggcagct cccgcgctat
    21541 tacgcaactc attctcaatc tggtagaagg ttcaccaatt aagctaattg atggcggaaa
    21601 acaaaaacgc tgctttactg atatccggga cggtatcgag gcgttgtacc acattatcga
    21661 aaatgcggga aatcgctgcg acggcgaaat tatcaacatt ggcaatcctg agaacgaagc
    21721 gagcattgag gaactgggcg agatgctgct ggcgagcttc gaaaaacatc cgctgcgcca
    21781 tcatttccca ccgtttgcgg gctttcgcgt tgtcgaaagt agctgctact acggcaaagg
    21841 atatcaggac gtagagcatc gtaaaccgag catccgcaat gcccaccgct gcctggactg
    21901 ggagccgaaa attgatatgc aggaaaccat cgacgaaacg ctggatttct tcctgcgcac
    21961 cgttgatctt acggataaac catcatgacc aaagtaggct tacgcattga tgtcgatgcc
    22021 tttcgtggca cccgtgaagg cgtgccgcgt ctgctggaaa tcttgagtaa gcataatatt
    22081 caggccagca tttttttcag cgtcggcccg gacaatatgg gccgccatct ctggcgactg
    22141 gtgaagccac agtttttgtg gaagatgctg cgctcaaacg cggcatcgct ttatggctgg
    22201 gatattttac tggcaggtac ggcctggcca ggtaaagaga ttggtcatgc caatgccgat
    22261 atcattcgtg aagcggctaa acatcacgaa gtcggtctgc acgcctggga tcaccatgcc
    22321 tggcaagccc gtagcggtaa ctgggatcgg caaacaatga tcgacgatat tgcacgcggt
    22381 cttcgcactc tggaagagat tatcggtcaa ccggtaacct gttctgccgc tgcgggctgg
    22441 cgtgccgacc agaaggtgat cgaagcaaaa gaagcgttcc atttgcgcta caacagcgat
    22501 tgtcgtgggg ccataccgtt ccgtccactg cttgaatcag gaaaccctgg cactgcgcaa
    22561 attccggtga ccttacccac ctgggatgaa gtgattggtc gggatgtgaa agcagaagat
    22621 tttaacggtt ggttactcaa ccgcatcctg cgagataaag gcacgccggt ttataccatt
    22681 catgcagaag ttgaaggctg cgcttatcag cataattttg tggatctcct caaacgcgca
    22741 gctcaggaag gcgtgacatt ttgcccttta agcgaactgt tatcagagac gttgccgctc
    22801 ggacaagttg ttcgcggaaa tattgccgga cgtgaaggct ggctgggttg ccaacaaatt
    22861 gcgggtagtc gctgatgaaa tcggtacgtt accttatcgg catcttcgcg tttattgcct
    22921 gctattacct gttaccgatc agcacgcgtc tgctctggca acccgatgaa acgcgttatg
    22981 cggaaatcag tcgagaaatg ctggcatccg gcgactggat tgtgccccat ctgttagggc
    23041 tacgttattt cgaaaaaccc attgccggat actggattaa cagcattggg caatggctat
    23101 ttggcgcgaa taactttggt gtgcgggcag gcgttatctt tgcgaccctg ttaactgccg
    23161 cgctggtgac ctggtttact ctgcgcttat ggcgcgataa acgtctggct ctactcgcca
    23221 cagtaattta tctctcattg tttattgtct atgccatcgg cacttatgcc gtgctcgatc
    23281 cgtttattgc attctggctg gtggcgggaa tgtgcagctt ctggctggca atgcaggcac
    23341 agacgtggaa aggcaaaagc gcaggatttt tactgctggg aatcacctgc ggcatggggg
    23401 tgatgaccaa aggttttctc gcccttgccg tgccggtatt aagcgtgctg ccatgggtag
    23461 cgacgcaaaa acgctggaaa gatctcttta tttacggctg gctggcggtt atcagttgcg
    23521 tactgacggt tctcccctgg ggactggcga tagcgcagcg ggagcctgac ttctggcact
    23581 attttttctg ggttgagcat attcaacgct ttgcactaga tgatgcccaa catagagctc
    23641 cgttctggta ctacgtgccg gtcatcattg ccggtagcct gccgtggctg ggattactcc
    23701 ccggtgcact gtacacaggc tggaaaaacc gcaagcattc cgcaaccgtc tatttgttga
    23761 gctggacgat aatgccgctg ctgtttttct ccgtcgctaa aggcaaattg cccacctata
    23821 ttctttcttg ctttgcacct ctggcaatgc tgttggcgca ttacgcgttg ctggcagcaa
    23881 aaaataatcc tctggcgctg cggattaatg gctggattaa catcgctttt ggcgtcactg
    23941 gcattattgc cacatttgtg atctccccct ggggaccaat gaacacgccg gtgtggcaaa
    24001 ccttcgagag ctataaagtc ttttgtgcct ggtcgatttt ttcgctatgg gcatttttcg
    24061 gctggtacac cttaacaaac gtcgaaaaga cctggtcttt cgccgcgctt tgcccgctgg
    24121 ggctggcgtt gctggtagga ttttcaattc ctgacagagt catggaagga aaacatccgc
    24181 aattttttgt cgagatgaca caagaatcac ttcagccaag ccgctatatt cttactgata
    24241 gcgtcggtgt tgccgcaggt ctggcatgga gcctgcaacg cgatgacatc atcatgtatc
    24301 gccagacagg tgagttgaaa tacggcctta attatccgga tgcgaaaggg agatttgtca
    24361 gcggtgatga gttcgcaaac tggcttaatc aacatcgtca ggaggggatt attacactcg
    24421 tgctttcggt tgaccgcgat gaagatatca acagtctcgc cattccgccc gcagatgcca
    24481 tcgttcgtca ggagcgtctg gtgctgattc agtatcgtcc caaatgatct ggctaacatt
    24541 agtctttgcc agcttgctta gcgttgccgg gcagttgtgt cagaaacagg caacctgctt
    24601 tgtggcgata aacaaacggc gcaaacatat cgtgctgtgg ctgggactgg cgctggcttg
    24661 tcttggtctt gccatgatgc tctggctgct ggtcttgcag aacgtaccgg taggcattgc
    24721 ctacccgatg ttaagtctga attttgtctg ggtgacgctg gctgcagtaa aactgtggca
    24781 cgaaccggta tcgccgcgtc actggtgtgg ggtggcgttc attattggtg gcattgtgat
    24841 cctcgggagt acggtgtaat gtgcctgata tggggattat tcagcgtcat tattgcctca
    24901 gttgcgcaat taagcctggg ttttgcggca agtcatctgc cgccgatgac gcacttatgg
    24961 gattttatcg cggctctgtt agcttttggc ctggatgccc ggatactgtt actcgggctg
    25021 ctgggttatc tgctttccgt tttttgttgg tataaaacgc tacataaact tgcgctaagc
    25081 aaagcctatg ccttgctcag tatgagttat gtgctggtat ggattgcttc aatggtactt
    25141 cccggctggg aagggacttt ttcgctgaaa gcactactgg gagtagcctg tattatgagc
    25201 gggttgatgc tgattttcct gcccacgaca aaacaacgtt actgagtttt ccctgccgct
    25261 gtacactgcc gttcccactc atcggggcta taactgcttg ccgagaggac tttcaccgta
    25321 tgcagctttt cacgattaat acaataaagc gcattctgca aaggcgagag taaatccccc
    25381 actttcacgg cgaagtcact cttcacttcg gcgatcattt ttatcgcgcc gccagcatcg
    25441 cagagcataa gtacatgtct gttatcttgt ttattgcagc acgatttttt gtgccagcca
    25501 ttccattgca ttatcctgtt tgctaagagt tttcacatca atttacagat taacagataa
    25561 actctttatc aacggcaatg gaatggcaga aatgagaagt gaaacggagg gttaattatt
    25621 gctgacgttg cacccactct tttagcgcct gacgtgaaat tttaataccg ccgtttttca
    25681 gctccggcgg taaagtcagc cagcgcaccg gttgttgaaa acgagccagc ttatctttca
    25741 cccattcgcc aagatcaacg gtttgctggt catactccac caccgctacc ggccgatgac
    25801 caaactcctt gtcggcaacg gaaacgataa acacctgcaa aaccgcagga tgtgcagcaa
    25861 taacacgctc gacttcttcc ggctgaatac cctcaccgcc actgaagaac agattgtcta
    25921 aacgtccgac aatggtcagc ttgccattat gcatctcacc gcgatcgcgc gtagcgtacc
    25981 agccttcgtc attaaccagt gaaaccagtt gcccgttacg ccagtaacct tctgccatac
    26041 tggcagcccg cagccacact tcattattaa cgattttcac ttcccgaccc ggcagcggca
    26101 aaccaacgtc tgccaggccg tcggcttctt tcgcacatac cgtggaggca aactcggtca
    26161 gaccatagcc gcaaaagcaa cgaatcccct gctcgcgcgc ctgttccgtc aactcgaccg
    26221 ggatagccgc gccgccaagt aacaccgctt tcagggaaac ggaactgcgg ttaaccagca
    26281 aacgccagag ttgtgttggc accagtgaag cgtgagtaca gcctgccagc atttgctcca
    26341 atggctgttt atcacgcacc gtcatccgcg caccagcgta taaccagcgc cacataattc
    26401 cctgaccgga gacgtgaaac agcggtaaag agagcaacca gtcatcgtga tcgccaaacg
    26461 gaatcagcga taacacacct tgcgcactgg caagatgggc ctgataagta tggacagcgg
    26521 ctttcggcaa accggtagaa cccgaggtca acgtcattga gcacagacgc gtcggctgcc
    26581 acgcagcggc atgtacgcct tcaaccagct gaatgtgcag cgacgttaat gccggaaacg
    26641 tgttttcccc atccggcacc agcgcaaatt gcagcgtcag attgggcagc aattcttcaa
    26701 gcaacggttg cggcagctga gggttcacgg gcaacacccg cgccccgcat tgcagtaacg
    26761 ccagccaggc gagcagcgtt tgcggcgtat tccacgcccg caacatcacg ccgctgccct
    26821 caaccacccc ctgcgccgca aagcccgtag ctaactcatc gacgcgagca caaagctcgc
    26881 gccagttgag ttgctcgtca ttaagacgta aggcgatggc ttctccccgc acttgccgcc
    26941 agtgacgcca cggccagtca gagaagatca taacaactgc tcctgtacat cagcgtccac
    27001 taacggcagt gggctccccg gccagcgacg aacctgctgc gcctgcatca gatccagcgt
    27061 gtccagtccc ggaatggtgt ctggcgttaa ccaggcagca atccgcgcca gttgcgttag
    27121 acctaagctc gactcaatgg aagaactgat caccgccgtc agccccagcg catgcgccgc
    27181 ctgtacctgc tcgcgtactt tttccagact gcccgtgagc gtgggtttga taactaccgc
    27241 gcgcacgccc tcttcagcca caaaggcaaa atccggctcg cgcaggcttt catcccaggc
    27301 aatggcaatg ccggtttcac gggcaaacgc tcgcgaatca tcgcgggttt tgcacggctc
    27361 ttcgagaaac gcgatgcggt ggcgataatc cgggttaaca tatttggcaa actgctgacc
    27421 tttcagcggt gtccaggcgc gatttgcgtc aagacgcaaa tgcagatccg gaattgcctc
    27481 cagcaacaga ttcaccacca tgccgtcgcg caccgcttcg tacaatccca ctttgacctt
    27541 cgccactttc tcgcccggca tatcggcaag tttgaggatc agatcgtccg gatcgccatt
    27601 acacagcggt gccgcacggt agttggctgc ttgcggcaac gtatctgtca gttctgccaa
    27661 tgcacagctt acgccaaagg ccacggaagg catctgcggt agctcgcaat cgcctgccag
    27721 ccagttattt acccaggcaa gcagcacgct ttgcgcctct tcccagtttt cctggctgaa
    27781 gcccggcagt ggggagatct ccccccaccc ttcgcgctcg ccctcacgca ggcaaacgta
    27841 cagcccgtcg cgggttttta accgcctgtc gcgcagaacc acccccgcgt ccatggggat
    27901 ctgccagcgg tatacctgcg cgctacgcat tacggattcc gtttaaattt gctgaaatca
    27961 ggctgacgtt tctggttgaa agcgttgcga ccttcctgac cttcttccgt catgtagaac
    28021 agcatggtgg cgttgcccgc cagctcctgc agccccgcct gcccgtcaca gtcggcgttc
    28081 agcgcagctt tcaggcagcg cagcgccatc gggctgtttt gcagcatttc gcggcaccaa
    28141 cggacggttt ctttttccag atccgccagc ggtaccacgg tgttgacaag gcccatatcc
    28201 agcgcctgtt ttgcgtcgta ctgacggcac aggaaccaga tttctcgcgc ttttttctgc
    28261 ccgacgatac gagccatgta ggaagcgccc cagccgccgt cgaaggaacc gactttcggg
    28321 ccagtctgac cgaagatggc attatctgct gcgatagtca ggtcgcacat catgtgcaga
    28381 acgtgaccac cgccgatgga gtagccagcc accatcgcga caaccggttt cggacaggta
    28441 cggatctgac gctggaagtc cagcacgttc aggtgatgta cgccggaatc atctttatag
    28501 ccgccgtaat caccacgcac tttctggtca ccaccggagc agaacgcttt atcgcctgcg
    28561 ccagtcagaa tgatcacgcc gatattgtcg tcataacgcg catctgccag tgcctggatc
    28621 atctctttta ccgtcagagg acggaaggca ttgcgcacct gcggacgatt aatggtgatt
    28681 tttgcgatac cgtcggtgga tttttcataa cgaatgtcct cgaaaccttc ggagcagtcg
    28741 tgccattcaa ccggtgcgta aggcattgct tcatcaggat aaatcatatt gtgtccttta
    28801 gtcagaaacg caagatctgc gccagacttg cgattacgcc agcgggattt tcccgatgcg
    28861 cgttatgtcc ggcgcgagga atgacatggc agtcggcagc cagttccgcc gccagggcgc
    28921 ggaatttgct gtcacgttca ccacataaat aataaaacgc aaatgtgcgg gcgctaaggt
    28981 tagcacgtaa atcaggctgg acggcgagag aagtcgcctc cagcatggcg gcaagggttg
    29041 cgccattatt gttgctgcgc agcgccacca gctcccggcg ttgatcgtca ttgagcgagg
    29101 caaaaacagg ctgttgatac cagtcggcaa ataccgccgt taacggttct gtgcgaaaac
    29161 gctgcgccca ttggcgatcg gaacgctgac gttccgcacg ttgttcagca ttttgcagcc
    29221 ccggatgccc gccttcgaca ataaccccac aaagccccgc cagcccctgg caagccgcca
    29281 tcatcgccac ccgtccgcca agcgagtacc ccaccagcca gaagtcaagg atgttgtaac
    29341 taaccaaggt tttacgcagt aagtcggtga catcatcaaa tccatcgacg ctaatcgccg
    29401 ccgaaccacc atgacctagg agatcaacat acaaccgtga gtagtcggca aacgcctcgc
    29461 ccacttcttg ccattcgtgg caatcgccgg aaaaaccgtg caaaaacacc agccagggta
    29521 aacctagttt tccgtgtttt gcctgcgcgt gcaggatcat aaatggctta cctgcgccag
    29581 aagttgctgg agcgtttgcg caccatcggt gtcgttaacc accatttcaa tcaccgtggt
    29641 ggttggcgtg cgccaggcgt cggcaaatgc cgtttcaagt tcctgccagt tttgcggatg
    29701 atgatatttc agctcgaaca tcgcggcggc gtgctcaaaa tggacgtttt gcggcatcag
    29761 atagaaacgc tcacgctcgc tttgcggcgt tggcaacagc gagaaaattt gcccgccgtt
    29821 gttgttcacc acaattaata ccagcggcgc agaaacctga cgcaataacg ccagcgcgtt
    29881 gagatcgtaa agcgcggaga gatcgcccac aatcgccagc gtcggtttgc cgcttgcccg
    29941 ctgaacgcct gcggcggtcg aaagcagtcc gtcgataccg ctggcccctc ggttgctgta
    30001 caccgggtaa cctgccggaa gttgcgaaag cgcatcaatc agacgtacca ccaggctgtt
    30061 accaacaaac aattgcccct gttcaggcag atagtcgctg atgcgatgcg ccagttgcgc
    30121 ttcgccaaac gcatcacggc gggcaataac cgcctgcatt gcctgttccg ccaggcgcgg
    30181 gatttcaacg caccagggct ggcgtttttc tgccggatgc agctccagcc agtcggcaat
    30241 attggcaatt aagcgacgtc cgcgatggtg tgccggatca agtcgccctt caatgtcatc
    30301 aacaatccag tactcttctg gttcacagct tgcctgccat tgcaggagcc gtttccccgt
    30361 caggctactt cccagttgca ccacaatttg cgcctgctgc agctcgctgg tcgctttggc
    30421 attgcctaac caaagatcgg cacacggcag cggctgcccg gtttgtgaca gcacatcgcc
    30481 aatcagcggc cagccaagag tttgcgccca cagggcaact tttttgccct cttccgcact
    30541 catgcgcccg gcaaccacca cgccgcgctt ttgtcgccag aagaaccagt cgcgctgttt
    30601 ttcactttcc agacgaggcg cttcacgcag ccacggtttg tcgtcctgcc accagtcacc
    30661 cagacgctgt tgccagctaa gcccggtatc gtccatttcg ccatacagcg gttcagcaaa
    30721 cgggcagttg atatggactc cccccgcatg aagcgtaccg agagcgtggt cgatggtaga
    30781 aaccagccaa cgtgcgggga tatcctgggt cgggcgcggc aatgaaatac tgtgcgtggg
    30841 gtgagaggcg aacattcccg gctggcgaat tgcctgattc gcgccgcagt caattagctc
    30901 cggcgggcga tcggcggtta agagaatcag tttttctccg gttaacccgg cttcaatcag
    30961 tgccggatag agatttgcca ccgccgtgcc ggaggtcaca atcaccgcca ccggctgctt
    31021 gctgactttc gccagcccca gcgccagatg ccccaaccca cgctcatcga aatgggtgtg
    31081 gtgaatgaat gcggaattct ccgccgccgc taacgttaac ggtgtagaac gcgagcctgg
    31141 ggcgatacag atgtgtctga cgccgtgacg cgttaatgct tccagaatga ccgccgccca
    31201 gcgtcggtta aatgcgctta ctgacatgag tttgtccggt atcaatattg cggctaagta
    31261 taaggagcta caaaaataga ttattgatat gaatcggtaa tgatgcgact cattactatt
    31321 ccatttgtaa taaagtacgc agccctgccg ctttgttgtc gatttcctgc cactcttgct
    31381 cggggtcgga accacggaca atgcccgcgc cagcatataa tcgcacgaca ttgccgctaa
    31441 tttttgctga gcgcagggaa acgcagaatt cgctttgttg taatgagaga tagcccgcag
    31501 aaccggcgta ccattctcgg gtgaacggtt cgtgacgggc gataaactgt cgcgccagat
    31561 cgcgcggtaa gccagcaact gctgccgttg gcagcaactg atgtaaacag atcacatcat
    31621 ccgctttgtt gagtgaagtc cagatacagc ggcgaagatg ctgcacttta cgcagacgca
    31681 gtacctgcgg cggtaaaaca tccagcgtct gggtatcggc ctgtaatcgt tggcagatat
    31741 cttccaccac cagcatgttc tcgcgctggt ttttatcatc cgccatcagc cactctccta
    31801 actgctgcgc ctgcttatca tcaggatgat ttgctactgt tcccgccagc gcttcagtac
    31861 gcagcgcttt gtcacgccgc cgccataacc gttccggtga agagccaaga aaagcatttt
    31921 cgccatcaaa ggccatgtaa aaatggtagc aattcagatt cagtcgacga ctggcagcca
    31981 tcatcgccgc cgcgttgacc ggacttgcga aatgcaggtc agttgcccga gcgagcacca
    32041 ctttgtcgag ctcaccttcg gcgatggttt tcgttgccag ttcgattaat tgcgtccagc
    32101 ccgttttgtc cggccagtgt tgttctcgcg tggtggttaa atgtaaccca ggcaagggct
    32161 tgatactcac cagtgtggcg ataaattctt ttgcctgaat cgcatcgtgc tgaagggagc
    32221 tttcgctgaa tagcgtcagc cgcagcgtgg ctttaccgcc acagcgtcgc cattccaggc
    32281 ggggtaaaag taaattgccc tgcgacgggt caaaagcatt cagcccccaa atgcgtaagt
    32341 cagcgtgttc cgggtgctgg cgaagaaaac gttgtgcctg gtccaacgac gtaaaacggg
    32401 taatcgcgcc caggacggca gcttcttcat caccattacg ttgttgccag tagaattgcg
    32461 ggtacgtttg ctgactggcc agccagctca aggcatcaaa agcgtctttg agagggaaag
    32521 gaatatcgat aacccgaatg ccgggtgtcg ccggaatctc ttgcgacaaa tggcgcagta
    32581 gattttccag cgccgtagta agtgattgca cgcggacctc tccctgttaa aagcctcaca
    32641 ttatacgggg tactacaaaa aaaatgcagt accccggtgt agggaggttt taacggcgtg
    32701 ccagcaacag tcctagtacc agcccgacgg ccgcgcccac accaattcct tgccagggtt
    32761 tttcgtggac gtagtcatca gcacgataaa cagcctgctt cgcccgatag taataactgt
    32821 ctgaagcctg gctaacccgt tttttcacat catccagcgc tttttctgca tgcgctttca
    32881 gctcaacata tttctgatcg gcgggatcgc cagaggagcg gagcacctct tccagtgttt
    32941 cactaagcag cgtcaggtcg tcatcgatac gtgtatcacc aaactgatta gacattctcg
    33001 ttctccattt gcgtaaaacc tgtcactaac tatagacaag agttgttgat tacgcctgaa
    33061 ttacctcgcg tgccatccca atgtgtggga taccatcttc ttcatagacc tccgtcacag
    33121 ggataaaacc gaaactctgg tagaagtttt gcagatgcgc ctgcgccccg aggtaaacag
    33181 gcttatcagg ccagtgatgc gtacatgttt cgagtgtttt actcatcagc tgctgcccca
    33241 ctttttcacc acgcaacgct tcactgacaa tcacccgacc tataacgacc ggctcaagat
    33301 catcatcact tttcagaatc ctcgcatacg ccaccagttc atcatttttc caaccgagaa
    33361 tatggcgatt atcccccgtc aaatcatcac catcgatatc ctgataaggg caattctgct
    33421 cgacaacaaa caccgcgcaa cgcagttgta ataaggcata aagctgggaa accgataatt
    33481 cagagtgatg cagatcttgc cattcaatca taatgctctc cttttagatg tatgccttat
    33541 actagccgga atattttttg aaacgtgatg aactcatgga attaattttt ttaggtactt
    33601 cagccggtgt gccaacccgc acgcgcaatg tcacggcaat attgcttaac ctgcaacatc
    33661 cgacccagag cggactttgg ttgtttgact gcggtgaagg cacccagcat cagctactgc
    33721 ataccgcctt taaccctgga aaactggaca agatttttat cagtcacctt catggcgatc
    33781 atctttttgg tttacccggc ttgctgtgca gtcgttctat gtcaggcatt atccaaccct
    33841 taacgattta tggtccgcaa ggtatccgtg aatttgtgga aaccgcgctg cggattagcg
    33901 gctcatggac cgattatccg ctggaaattg tcgaaattgg cgctggcgaa attctcgatg
    33961 atggcctgcg caaagtaacc gcttatccgc tggaacaccc actggaatgt tatggctatc
    34021 gtattgaaga acatgataaa ccgggtgcat taaatgccca ggcattaaaa gctgctggcg
    34081 tgccgcctgg cccactgttt caggaattaa aagcgggcaa aacaatcacg ctggaagatg
    34141 gaaggcagat taacggcgca gattacttag ctgctccagt gccaggtaaa gcgctcgcta
    34201 ttttcggcga taccggcccc tgcgatgccg cacttgacct ggctaaaggt gtcgatgtca
    34261 tggtgcacga agcgacgctg gatataacca tggaagccaa agccaatagt cgcggccata
    34321 gctctacacg ccaggctgcg acactagccc gtgaggctgg agtcggcaag ctaatcatta
    34381 cccacgtcag ctcgcgctat gatgacaaag gttgtcaaca cctgttacgt gaatgcaggt
    34441 caattttccc ggcgactgaa ctggcgaatg atttcaccgt gtttaacgtt taactctgct
    34501 tattattaaa aacagggtga aacttgccct gttatagcaa cccgcgctac atatatccac
    34561 ccacacaggc atattgagcg att
//
LOCUS       DAJTRC010000035        34447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.035, whole genome shotgun sequence.
ACCESSION   DAJTRC010000035 DAJTRC010000000
VERSION     DAJTRC010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.035"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..334)
                     /locus_tag="OO357_001814"
     CDS             complement(<1..334)
                     /locus_tag="OO357_001814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886780.1"
                     /translation="MAHRLLIGKGMITLNLKRIFLTLTLLPLFAVAADDCALSDPTLT
                     VQAYTVNPQTERVKMYWQKANGEAWGTLHALLADINSQGQVQMAMNGGIYDESYAPLG
                     LYIENGQQK"
     gene            704..1654
                     /gene="corA"
                     /locus_tag="OO357_001815"
     CDS             704..1654
                     /gene="corA"
                     /locus_tag="OO357_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HCR5886781.1"
                     /translation="MLSAFQLENNRLTRLEVEESQPLVNAVWIDLVEPDDDERLRVQS
                     ELGQSLATRPELEDIEASARFFEDDDGLHIHSFFFFEDAEDHAGNSTVAFTIRDGRLF
                     TLRERELPAFRLYRMRARSQSMVDGNAYELLLDLFETKIEQLADEIENIYSDLEQLSR
                     VIMEGHQGDEYDEALSTLAELEDIGWKVRLCLMDTQRALNFLVRKARLPGGQLEQARE
                     ILRDIESLLPHNESLFQKVNFLMQAAMGFINIEQNRIIKIFSVVSVVFLPPTLVASSY
                     GMNFEFMPELKWSFGYPGAIIFMILAGLAPYLYFKRKNWL"
     gene            complement(1695..2075)
                     /locus_tag="OO357_001816"
     CDS             complement(1695..2075)
                     /locus_tag="OO357_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709623.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2628 domain-containing protein"
                     /protein_id="HCR5886782.1"
                     /translation="MSKEYMNDDSLSEKWKYRFNFYDQHGFPGFWGATPEYKAAFKAL
                     KVRQRLTIQMNFIAFFCSWIYLFVLGLWKKAIIVLLLGILSLFVGALIGVNILGIAVA
                     AYVAVNTNKWFYEKEVKGLNTWSL"
     gene            complement(2089..2505)
                     /locus_tag="OO357_001817"
     CDS             complement(2089..2505)
                     /locus_tag="OO357_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886783.1"
                     /translation="MLTGDSHKDVKFMLRMFIPTSNGKISRHRYIFLFILINFIFAFL
                     IIFFNDGEAGFLVIVSTIALHYLVINMNCQRLRDSGFIYIKIYVFGTLAVYIISIITM
                     IAEDFACSGNGSMIFLICYFSTFSMLMLAPTDSSKQ"
     gene            complement(2564..3454)
                     /gene="rarD"
                     /locus_tag="OO357_001818"
     CDS             complement(2564..3454)
                     /gene="rarD"
                     /locus_tag="OO357_001818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter RarD"
                     /protein_id="HCR5886784.1"
                     /translation="MDAKQTRQGVLLALAAYFIWGIAPAYFKLIYYVPADEILTHRVI
                     WSFFFMVVLMSICRQWSYLKTLIQTPQKIFMLAVSAVLIGGNWLLFIWAVNNHHMLEA
                     SLGYFINPLVNIVLGMIFLGERFRRMQWLAVILAICGVLVQLWTFGSLPIIALGLAFS
                     FAFYGLVRKKIAVEAQTGMLIETMWLLPVAAIYLFAIADSSTSHMGQNPMSLNLLLIA
                     AGIVTTVPLLCFTAAATRLRLSTLGFFQYIGPTLMFLLAVTFYGEKPGADKMVTFTFI
                     WVALAIFVMDAIYTQRRTSK"
     gene            complement(3506..3973)
                     /locus_tag="OO357_001819"
     CDS             complement(3506..3973)
                     /locus_tag="OO357_001819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312777.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HCR5886785.1"
                     /translation="MSAVLTAEQALKLVGEMFVYHMPFNRALGMELERYEKEFAQLAF
                     KNQPMMVGNWAQSILHGGVIASALDVAAGLVCVGSTLTRHETISEDELRQRLSRMGTI
                     DLRVDYLRPGRGERFTATSSLLRAGNKVAVARVELHNEEQLYIASATATYMVG"
     gene            4138..5007
                     /gene="pldA"
                     /locus_tag="OO357_001820"
     CDS             4138..5007
                     /gene="pldA"
                     /locus_tag="OO357_001820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase A"
                     /protein_id="HCR5886786.1"
                     /translation="MRTLQGWLLPVFMLPMAVYAQEATVKEVHDAPAVRGSIIANMLQ
                     EHDNPFTLYPYDTNYLIYTQTSDLNKEAIASYDWAENARKDEVKFQLSLAFPLWRGIL
                     GPNSVLGASYTQKSWWQLSNSEESSPFRETNYEPQLFLGFATDYRFAGWTLRDVEMGY
                     NHDSNGRSDPTSRSWNRLYTRLMAENGNWLVEVKPWYVVGNTDDNPDITKYMGYYQLK
                     IGYHLGDAVLSAKGQYNWNTGYGGAELGLSYPITKHVRLYTQVYSGYGESLIDYNFNQ
                     TRVGVGVMLNDLF"
     gene            5140..6969
                     /gene="recQ"
                     /locus_tag="OO357_001821"
     CDS             5140..6969
                     /gene="recQ"
                     /locus_tag="OO357_001821"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026263.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HCR5886787.1"
                     /translation="MAQAEVLNLESGAKQVLQETFGYQQFRPGQEEIIDTVLSGRDCL
                     VVMPTGGGKSLCYQIPALLLNGLTVVVSPLISLMKDQVDQLQANGVAAACLNSTQTRE
                     QQLEVMTGCRTGQIRLLYIAPERLMLDNFLEHLAHWNPVLLAVDEAHCISQWGHDFRP
                     EYAALGQLRQRFPTLPFMALTATADDTTRQDIVRLLGLNDPLIQISSFDRPNIRYMLM
                     EKFKPLDQLMRYVQEQRGKSGIIYCNSRAKVEDTAARLQSRGISAAAYHAGLENNVRA
                     DVQEKFQRDDLQIVVATVAFGMGINKPNVRFVVHFDIPRNIESYYQETGRAGRDGLPA
                     EAMLFYDPADMAWLRRCLEEKPQGQLQDIERHKLNAMGAFAEAQTCRRLVLLNYFGEG
                     RQEPCGNCDICLDPPKQYDGSTDAQIALSTIGRVNQRFGMGYVVEVIRGANNQRIRDY
                     GHDKLKVYGMGRDKSHEHWVSVIRQLIHLGLVTQNIAQHSALQLTEAARPVLRGESSL
                     QLAVPRIVALKPKAMQKSFGGNYDRKLFAKLRKLRKSIADESNVPPYVVFNDATLIEM
                     AEQMPITASEMLSVNGVGMRKLERFGKPFMALIRAHVDGDDEE"
     gene            7033..7653
                     /gene="rhtC"
                     /locus_tag="OO357_001822"
     CDS             7033..7653
                     /gene="rhtC"
                     /locus_tag="OO357_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709629.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine export protein RhtC"
                     /protein_id="HCR5886788.1"
                     /translation="MLMLFLTVAMVHIVALMSPGPDFFFVSQTAVSRSRKEAMMGVLG
                     ITCGVMVWAGIALLGLHLIIEKMAWLHTLIMVGGGLYLCWMGYQMLRGALKKEAVSAP
                     APQVELAKSGRSFLKGLLTNLANPKAIIYFGSVFSLFVGDNVGTTERWGIFALIIVET
                     LAWFTVVASLFALPQMRRGYQRLAKWIDGFAGALFAGFGIHLIISR"
     gene            complement(7715..8335)
                     /gene="rhtB"
                     /locus_tag="OO357_001823"
     CDS             complement(7715..8335)
                     /gene="rhtB"
                     /locus_tag="OO357_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine/homoserine lactone efflux protein"
                     /protein_id="HCR5886789.1"
                     /translation="MTLEWWFAYLLTSIILSLSPGSGAINTMTTSLNHGYRGAVASIA
                     GLQTGLAIHIVLVGVGLGTLFSRSVIAFEVLKWAGAAYLIWLGIQQWRAAGAIDLKSL
                     ASTQSRRHLFQRAVFVNLTNPKSIVFLAALFPQFIMPQQPQLMQYIVLGVTTIVVDII
                     VMIGYATLAQRIALWIKGPKQMKALNKIFGSLFMLVGALLASARHA"
     gene            8446..9468
                     /gene="pldB"
                     /locus_tag="OO357_001824"
     CDS             8446..9468
                     /gene="pldB"
                     /locus_tag="OO357_001824"
                     /EC_number="3.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase L2"
                     /protein_id="HCR5886790.1"
                     /translation="MFQQQKDWETRENAFAAFTMGPLTDFWRQRDEAEFTGVDDIPVR
                     FVRFRAQHHDRVVVICPGRIESYVKYAELAYDLFHLGFDVLIIDHRGQGRSGRLLADP
                     HLGHVNRFNDYVDDLAAFWQQEVQPGPWRKRYILAHSMGGAISTLFLQRHPGVCDAIA
                     LTAPMFGIVIRMPSFMARQILNWAEAHPRFRDGYAIGTGRWRALPFAINVLTHSRQRY
                     RRNLRFYADDPTIRVGGPTYHWVRESILAGEQVLAGAGDDATQTLLLQAEEERVVDNR
                     MHDRFCELRTAAGHPVEGGRPLVIKGAYHEILFEKDAMRSVALHAIVDFFNRHNSPSG
                     NRSTEV"
     gene            9476..10276
                     /gene="yigL"
                     /locus_tag="OO357_001825"
     CDS             9476..10276
                     /gene="yigL"
                     /locus_tag="OO357_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709632.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar/pyridoxal phosphate phosphatase YigL"
                     /protein_id="HCR5886791.1"
                     /translation="MYQVVASDLDGTLLSPDHTLSPYAKETLKLLTARGINFVFATGR
                     HHVDVGQIRDNLEIKSYMITSNGARVHDLDGNLIFAHNLDRDIASDLFGVVNDNPDII
                     TNVYRDDEWFMNRHRPEEMRFFKEAVFKYALYEPGLLEPEGISKVFFTCDSHEQLLPL
                     EQAINARWGDRVNVSFSTLTCLEVMAGGVSKGHALEAVAKKLGYSLKDCIAFGDGMND
                     AEMLSMAGKGCIMGSAHQRLKDLHPELEVIGTNADDAVPHYLRKLYLS"
     gene            10352..11251
                     /gene="bioP"
                     /locus_tag="OO357_001826"
     CDS             10352..11251
                     /gene="bioP"
                     /locus_tag="OO357_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter"
                     /protein_id="HCR5886792.1"
                     /translation="MALLIITTILWAFSFSFYGEYLAGHVDSYFAVLVRVGLAALVFL
                     PFLRTRSNSLKTVGLYMLVGAMQLGVMYMLSFRAYLYLTVSELLLFTVLTPLYITLIY
                     DIMSKRRLRWGYAFSALLAVIGAGIIRYDQVTDHFWTGLLLVQLSNITFAIGMVGYKR
                     LMETRPMPQYNAFAWFYLGAFLVAVIAWFLLGNAQKMPQTTLQWGILVFLGVVASGIG
                     YFMWNYGATQVDAGTLGIMNNMHVPAGLLVNLAIWHQQPHWPTFITGALVILASLWVH
                     RKWVAPRSSQTADDRRRDCALSE"
     gene            complement(11139..12092)
                     /gene="metR"
                     /locus_tag="OO357_001827"
     CDS             complement(11139..12092)
                     /gene="metR"
                     /locus_tag="OO357_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MetR"
                     /protein_id="HCR5886793.1"
                     /translation="MIEVKHLKTLQALRNCGSLAAAAATLHQTQSALSHQFSDLEQRL
                     GFRLFVRKSQPLRFTPQGEILLQLANQVLPQISQALQACNEPQQTRLRIAIECHSCIQ
                     WLTPALGNFHKNWPQVEMDFKSGVTFDPQPALQQGELDLVMTSDILPRSGLHYSPMFD
                     YEVRLVLAPDHPLAAKTRITPEDLASETLLIYPVQRSRLDVWRHFLQPAGVSPSLKSV
                     DNTLLLIQMVAARMGIAALPHWVVESFERQGLVVTKTLGEGLWSRLYAAVRDGEQRQP
                     VTEAFIRSARNHACDHLPFVKSAERPTYDAPTVRPGSPARL"
     gene            12328..14589
                     /gene="metE"
                     /locus_tag="OO357_001828"
     CDS             12328..14589
                     /gene="metE"
                     /locus_tag="OO357_001828"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HCR5886794.1"
                     /translation="MTILNHTLGFPRVGLRRELKKAQESYWAGNSTREELLTVGRELR
                     ARHWDQQKQAGIDLLPVGDFAWYDHVLTTSLLLGNVPPRHQNKDGSVDIDTLFRIGRG
                     RAPTGEPAAAAEMTKWFNTNYHYMVPEFVKGQQFKLTWTQLLEEVDEALALSHNVKPV
                     LLGPVTYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELP
                     QAWLDAYKPAYDALQGQVKLLLTTYFEGVTPNLDTITALPVQGLHVDLVHGKDNVVEL
                     HKRLPSDWLLSAGLINGRNVWRADLTEKYAQIKDIVGKRDLWVASSCSLLHSPIDLSV
                     ETRLDAEVKSWFAFALQKCHELALLRDALNSGDTAALAEWSAPIQARRHSTRVHNPAV
                     EKRLAAITAQDSQRANVYEVRAEAQRARFKLPAWPTTTIGSFPQTTEIRTLRLDFKKG
                     NLDANNYRTGIAEHIKQAIVEQERLGLDVLVHGEAERNDMVEYFGEHLDGFVFTQNGW
                     VQSYGSRCVKPPIVIGDISRPAPITVEWAKYAQSLTDKPVKGMLTGPVTILCWSFPRE
                     DVSRETIAKQIALALRDEVADLEAAGIGIIQIDEPALREGLPLRRSDWDAYLQWGVEA
                     FRINAAVAKDDTQIHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEEFDY
                     PNEIGPGVYDIHSPNVPSVEWIEALLKKAAKRIPAERLWVNPDCGLKTRGWPETRAAL
                     ANMVQAAQNLRRG"
     gene            complement(14628..15443)
                     /locus_tag="OO357_001829"
     CDS             complement(14628..15443)
                     /locus_tag="OO357_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HCR5886795.1"
                     /translation="MATTQQSGFAPAASPLASTIVQTPDDAIVAGFTSIPSQGDNMPA
                     YHARPKQSDGPLPVVIVVQEIFGVHEHIRDICRRLALEGYLAIAPELYFREGDPNDFA
                     DIPTLLSGLVAKVPDSQVLADLDHVASWASRNGGDVHRLMITGFCWGGRITWLYAAHN
                     PQLKAAVAWYGKLTGDKSLNSPKQPVDIATDLNAPVLGLYGGQDNSIPQESVETMRQA
                     LRAANAKAEIIVYPDAGHAFNADYRPSYHAESAKDGWQRMLEWFKQYGGKKSL"
     gene            15705..16466
                     /gene="udp"
                     /locus_tag="OO357_001830"
     CDS             15705..16466
                     /gene="udp"
                     /locus_tag="OO357_001830"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709637.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HCR5886796.1"
                     /translation="MSKSDVFHLGLTKNDLQGATLAIVPGDPDRVEKIAALMDKPVKL
                     ASHREFTTWRAELDGKPVIVCSTGIGGPSTSIAVEELAQLGIRTFLRIGTTGAIQPHI
                     NVGDVLVTTASVRLDGASLHFAPLEFPAVADFECTTALVEAAKSIGATTHVGVTVSSD
                     TFYPGQERYDTYSGRVVRHFKGSMEEWQAMGVMNYEMESATLLTMCASQGLRAGMVAG
                     VIVNRTQQEIPNAETMKQTESHAVKIVVEAARRLL"
     gene            16607..18034
                     /gene="rmuC"
                     /locus_tag="OO357_001831"
     CDS             16607..18034
                     /gene="rmuC"
                     /locus_tag="OO357_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombination protein RmuC"
                     /protein_id="HCR5886797.1"
                     /translation="MDFSIMVYAVIALVGVAIGWLFASYQHAQQKAEQLAEREEMVAE
                     LSAAKQQITQSEHWRAECELLNNEVRSLQSINTSLEADLREVTTRMEAAQQHADDKIR
                     QMINSEQRLSEQFENLANRIFEHSNRRVDEQNRQSLNSLLSPLREQLDGFRRQVQDSF
                     GKEAQERHTLTHEIRNLQQLNAQMAQEAINLTRALKGDNKTQGNWGEVVLTRVLEASG
                     LREGYEYETQVSIENDARSRMQPDVIVRLPQGKDVVIDAKMTLVAYERYFNAEDDYTR
                     ESALQEHIASVRNHIRLLGRKDYQQLPGLRTLDYVLMFIPVEPAFLLALDRQPELITE
                     ALKNNIMLVSPTTLLVALRTIANLWRYEHQSRNAQQIADRASKLYDKMRLFIDDMSAI
                     GQSLDKAQDNYRQAMKKLSSGRGNVLAQAEAFRGLGVEIKREINPDLAEQAVSQDEEY
                     RLRSVPEQPNDEAYQRDDEYNQQSR"
     gene            18129..18884
                     /gene="ubiE"
                     /locus_tag="OO357_001832"
     CDS             18129..18884
                     /gene="ubiE"
                     /locus_tag="OO357_001832"
                     /EC_number="2.1.1.163"
                     /EC_number="2.1.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional demethylmenaquinone
                     methyltransferase/2-methoxy-6-polyprenyl-1,4-benzoquinol
                     methylase UbiE"
                     /protein_id="HCR5886798.1"
                     /translation="MVDKSQETTHFGFQTVAKEQKADMVAHVFHSVASKYDVMNDLMS
                     FGIHRLWKRFTIDCSGVRRGQTVLDLAGGTGDLTAKFSRLVGETGKVVLADINESMLK
                     MGREKLRNIGVIGNVEYVQANAEALPFPDNTFDCITISFGLRNVTDKDKALRSMYRVL
                     KPGGRLLVLEFSKPIIEPLSKAYDAYSFHVLPRIGSLVANDADSYRYLAESIRMHPDQ
                     DTLKAMMQDAGFESVDYYNLTAGVVALHRGYKF"
     gene            18898..19503
                     /gene="ubiJ"
                     /locus_tag="OO357_001833"
     CDS             18898..19503
                     /gene="ubiJ"
                     /locus_tag="OO357_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis protein UbiJ"
                     /protein_id="HCR5886799.1"
                     /translation="MPFKPLVTAGIESLLNTFLYRSPALKTARSRLLGKVLRVEVKGF
                     STSLILVFSERQVDVLGEWAGDADCTVIAYASVLPKLRDRQQLTALIRSGELEVQGDI
                     QVVQNFVALADLAEFDPAELLAPYTGDIAAEGISKAMRGGAKFLHHGIKRQQRYVAEA
                     ITEEWRMAPGPLEVAWFAEETAAVERAVDALTKRLEKLEAK"
     gene            19500..21140
                     /gene="ubiB"
                     /locus_tag="OO357_001834"
     CDS             19500..21140
                     /gene="ubiB"
                     /locus_tag="OO357_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone biosynthesis regulatory protein
                     kinase UbiB"
                     /protein_id="HCR5886800.1"
                     /translation="MTPGEVRRLYFIIRTFLSYGLDELIPKMRITLPLRLWRYSLFWM
                     PNRHKDKLLGERLRLALQELGPVWIKFGQMLSTRRDLFPPHIADQLALLQDKVAPFDG
                     KLAKQQIEAAMGGLPVEAWFDDFEIKPLASASIAQVHTARLKSNGKEVVIKVIRPDIL
                     PVIKADLKLIYRLARWVPRLLPDGRRLRPTEVVREYEKTLIDELNLLRESANAIQLRR
                     NFEDSPMLYIPEVYPDYCSEGMMVMERIYGIPVSDVAALEKNGTNMKLLAERGVQVFF
                     TQVFRDSFFHADMHPGNIFVSYEHPENPKYIGIDCGIVGSLNKEDKRYLAENFIAFFN
                     RDYRKVAELHVDSGWVPPDTNVEEFEFAIRTVCEPIFEKPLAEISFGHVLLNLFNTAR
                     RFNMEVQPQLVLLQKTLLYVEGVGRQLYPQLDLWKTAKPFLESWIKDQVGIPALVRAF
                     KEKAPFWVEKMPELPELVYDSLRQGKYLQHSVDKIARELQSNHVRQGQSRYFLGIGAT
                     LVLSGTFLLVSRPEWGLMPGWLMAGGLIAWFVGWRKTR"
     gene            21219..21488
                     /gene="tatA"
                     /locus_tag="OO357_001835"
     CDS             21219..21488
                     /gene="tatA"
                     /locus_tag="OO357_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatA"
                     /protein_id="HCR5886801.1"
                     /translation="MGGISIWQLLIIAVIVVLLFGTKKLGSIGSDLGASIKGFKKAMS
                     DDEPKQDKTSQDADFTAKTIADKQADTNQEQAKTEDAKRHDKEQV"
     gene            21492..22007
                     /gene="tatB"
                     /locus_tag="OO357_001836"
     CDS             21492..22007
                     /gene="tatB"
                     /locus_tag="OO357_001836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatB"
                     /protein_id="HCR5886802.1"
                     /translation="MFDIGFSELLLVFIIGLVVLGPQRLPVAVKTVAGWIRALRSLAT
                     TVQNELTQELKLQEFQDSLKKVEKASLTNLTPELKASMDELRQAAESMKRSYVANDPE
                     KASDEAHTIHNPVVKDNEAAHEGVTPAAAQTQASSPEQKPETTPEPVVKPAADAEPKT
                     AAPSPSSSDKP"
     gene            22010..22786
                     /gene="tatC"
                     /locus_tag="OO357_001837"
     CDS             22010..22786
                     /gene="tatC"
                     /locus_tag="OO357_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase subunit
                     TatC"
                     /protein_id="HCR5886803.1"
                     /translation="MSVEDTQPLITHLIELRKRLLNCIIAVIVIFLCLVYFANDIYHL
                     VSAPLIKQLPQGSTMIATDVASPFFTPIKLTFMVSLILSAPVILYQVWAFIAPALYKH
                     ERRLVVPLLVSSSLLFYIGMAFAYFVVFPLAFGFLANTAPEGVQVSTDIASYLSFVMA
                     LFMAFGVSFEVPVAIVLLCWMGITSSEDLRKKRPYVLVGAFVVGMLLTPPDVFSQTLL
                     AIPMYCLFEIGVFFSRFYVGKGRNREEENDAEAESEKTEE"
     gene            22828..23610
                     /gene="tatD"
                     /locus_tag="OO357_001838"
     CDS             22828..23610
                     /gene="tatD"
                     /locus_tag="OO357_001838"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' ssDNA/RNA exonuclease TatD"
                     /protein_id="HCR5886804.1"
                     /translation="MFDIGVNLTSSQFAKDRDDVVARAFDAGVNGLLITGTNLRESQQ
                     AQKLARQYSSCWSTAGVHPHDSSQWQAATEEAIIELAAQPEVVAIGECGLDFNRNFST
                     PEEQERAFVAQLRIAAELNMPVFMHCRDAHERFMTLLEPWLDKLPGAVLHCFTGTREE
                     MQACVARGIYIGITGWVCDERRGLELRELLPLIPAEKLLIETDAPYLLPRDLTPKPSS
                     RRNEPAHLPHILQRIAHWRGEDAAWLAATTDANVKTLFGIAF"
     gene            complement(23607..24095)
                     /gene="rfaH"
                     /locus_tag="OO357_001839"
     CDS             complement(23607..24095)
                     /gene="rfaH"
                     /locus_tag="OO357_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription/translation regulatory transformer
                     protein RfaH"
                     /protein_id="HCR5886805.1"
                     /translation="MQSWYLLYCKRGQLQRAQEHLERQAVNCLAPMITLEKIVRGKRT
                     AVSEPLFPNYLFVEFDPEVIHTTTISATRGVSHFVRFGASPAIVPSAVIHQLSVYKPK
                     DIVDPAPPYPGDKVIITEGAFEGFQAIFTEPDGEARSMLLLNLINKEIKHSVKNTEFR
                     KL"
     gene            24262..25755
                     /gene="ubiD"
                     /locus_tag="OO357_001840"
     CDS             24262..25755
                     /gene="ubiD"
                     /locus_tag="OO357_001840"
                     /EC_number="4.1.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-polyprenylbenzoate decarboxylase"
                     /protein_id="HCR5886806.1"
                     /translation="MDAMKYNDLRDFLTLLEQQGELKRITLPVDPHLEITEIADRTLR
                     AGGPALLFENPKGYSMPVLCNLFGTPKRVAMGMGQEDVSALREVGKLLAFLKEPEPPK
                     GFRDLFDKLPQFKQVLNMPTKRLRGAPCQQKIVSGDDVDLNRIPIMTCWPEDAAPLIT
                     WGLTVTRGPHKERQNLGIYRQQLIGKNKLIMRWLSHRGGALDYQEWCAAHPGERFPVS
                     VALGADPATILGAVTPVPDTLSEYAFAGLLRGTKTEVVKCISNDLEVPASAEIVLEGY
                     IDPGEMAPEGPYGDHTGYYNEVDNFPVFTVTHITQREDAIYHSTYTGRPPDEPAVLGV
                     ALNEVFVPILQKQFPEIVDFYLPPEGCSYRLAVVTIKKQYAGHAKRVMMGVWSFLRQF
                     MYTKFVIVCDDDVNARDWNDVIWAITTRMDPARDTVLVENTPIDYLDFASPVSGLGSK
                     MGLDATNKWPGETQREWGRPIKKDPDVVAHIDAIWDELAIFNNGKSA"
     gene            25801..26502
                     /gene="fre"
                     /locus_tag="OO357_001841"
     CDS             25801..26502
                     /gene="fre"
                     /locus_tag="OO357_001841"
                     /EC_number="1.5.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-flavin reductase"
                     /protein_id="HCR5886807.1"
                     /translation="MTTLSCKVTSVAAITDTVYRVRIVPDAAFSFRAGQYLMVVMDER
                     DKRPFSMASTPDEKGFIELHIGASEINLYAKAVMDRILKDHQIVVDLPHGEAWLRDDE
                     ERPMILIAGGTGFSYARSILLTALARNPNRDITIYWGGREEQHLYDLCELEALSLKHL
                     GLQVVPVVEQPEAGWRGRTGTVLTAVLQDHGTLAEHDIYIAGRFEMAKIARDLFCSER
                     NAREDRLFGDAFAFI"
     gene            complement(26694..27857)
                     /gene="fadA"
                     /locus_tag="OO357_001842"
     CDS             complement(26694..27857)
                     /gene="fadA"
                     /locus_tag="OO357_001842"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadA"
                     /protein_id="HCR5886808.1"
                     /translation="MEQVVIVDAIRTPMGRSKGGAFRNVRAEDLSAHLMRSLLARNPA
                     LEAAALDDIYWGCVQQTLEQGFNIARNAALLAEVPHSVPAVTVNRLCGSSMQALHDAA
                     RMIMTGDTQACLVGGVEHIGHVPMSHGVDFHPGLSRNVAKAAGMMGLTAEMLARMHGI
                     SREMQDAFAARSHARAWAATQSAAFKNEIIPTGGHDADGVLKQFNYDEVIRPETTVEA
                     LATLRPAFDPVNGTVTAGTSSALSDGAAAMLVMSESRAHELGLKPRARVRSMAVVGCD
                     PSIMGYGPVPASKLALKKAGLSASDIGVFEMNEAFAAQILPCIKDLGLMEQIDEKINL
                     NGGAIALGHPLGCSGARISTTLLNLMERKDVQFGLATMCIGLGQGIATVFERV"
     gene            complement(27867..30056)
                     /gene="fadB"
                     /locus_tag="OO357_001843"
     CDS             complement(27867..30056)
                     /gene="fadB"
                     /locus_tag="OO357_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadB"
                     /protein_id="HCR5886809.1"
                     /translation="MLYKGDTLYLDWLEDGIAELVFDAPGSVNKLDTATVASLGEAIG
                     VLEQQSDLKGLLLRSNKAAFIVGADITEFLSLFLVPEEQLSQWLHFANSVFNRLEDLP
                     VPTIAAVNGYALGGGCECVLATDYRLATPDLRIGLPETKLGIMPGFGGSVRMPRMLGA
                     DSALEIIAAGKDVGADQALKIGLVDGVVKAEKLIEGAMAILRQAINGDLDWKAKRQPK
                     QEPLKLSKIEATMSFTIAKGMVAQTAGKHYPAPITAVKTIEAAARFGREEALNLENKS
                     FVPLAHTNEARALVGIFLNDQYVKGKAKKLTKDVETPKQAAVLGAGIMGGGIAYQSAW
                     KGVPVVMKDINDKSLTLGMTEAAKLLNKQLERGKIDGLKLAGVISTIHPTLDYAGFDR
                     VDIVVEAVVENPKVKKAVLAETEQKVRQDTVLASNTSTIPISELANALERPENFCGMH
                     FFNPVHRMPLVEIIRGEKSSDETIAKVVAWASKMGKTPIVVNDCPGFFVNRVLFPYFA
                     GFSQLLRDGADFRKIDKVMEKQFGWPMGPAYLLDVVGIDTAHHAQAVMAAGFPQRMQK
                     DYRDAIDALFDANRFGQKNGLGFWRYKEDSKGKPKKEEDAAVEDLLAEVSQPKRDFSE
                     EEIIARMMIPMVNEVVRCLEEGIIATPAEADMALVYGLGFPPFHGGAFRWLDTLGSAK
                     YLDMAQQYQHLGPLYEVPEGLRNKARHNEPYYPPVEPARPVGDLKTA"
     gene            30246..31577
                     /gene="pepQ"
                     /locus_tag="OO357_001844"
     CDS             30246..31577
                     /gene="pepQ"
                     /locus_tag="OO357_001844"
                     /EC_number="3.4.13.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro dipeptidase"
                     /protein_id="HCR5886810.1"
                     /translation="MESLASLYKNHIATLQERTRDALARFKLDALLIHSGELFNVFLD
                     DHPYPFKVNPQFKAWVPVTQVPNCWLLVDGVNKPKLWFYLPVDYWHNVEPLPTSFWTE
                     DVEVIALPKADGIGSLLPAARGNIGYIGPVPERALQLGIEASNINPKGVIDYLHYYRS
                     FKTEYELACMREAQKMAVNGHRAAEEAFRSGMSEFDINIAYLTATGHRDTDVPYSNIV
                     ALNEHAAVLHYTKLDHQAPEEMRSFLLDAGAEYNGYAADLTRTWSAKSDNDYAQLVKD
                     VNDEQLALIATMKAGVSYVDYHIQFHQRIAKLLRKHQIITDMSEEAMVENDLTGPFMP
                     HGIGHPLGLQVHDVAGFMQDDSGTHLAAPAKYPYLRYTRILQPGMVLTIEPGIYFIES
                     LLAPWREGQFSKHFNWQKIEALKPFGGIRIEDNVVIHENNVENMTRDLKLA"
     gene            31577..32191
                     /locus_tag="OO357_001845"
     CDS             31577..32191
                     /locus_tag="OO357_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709652.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMPACT family protein"
                     /protein_id="HCR5886811.1"
                     /translation="MESWLIPAAPVTVVEEIKKSRFITMLAHTDGVEAAKAFVESVRA
                     EHPDARHHCVAWVAGAPDDSQQLGFSDDGEPAGTAGKPMLAQLMGSGVGEITAVVVRY
                     YGGILLGTGGLVKAYGGGVNQALRQLTTQRKTPLTEYTLQCEYSQLTGIEALLGQCDG
                     KIINSDYQAFVLLRVALPAAKVAEFSAKLADFSRGSLQLLAIEE"
     gene            32230..33681
                     /gene="trkH"
                     /locus_tag="OO357_001846"
     CDS             32230..33681
                     /gene="trkH"
                     /locus_tag="OO357_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004146450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkH"
                     /protein_id="HCR5886812.1"
                     /translation="MHFRAITRIVGLLVILFSGTMIIPGLVALIYRDGAGRAFTQTFF
                     VALAIGSMLWWPNRKEKGELKSREGFLIVVLFWTVLGSVGALPFIFSESPNLTITDAF
                     FESFSGLTTTGATTLVGLDSLPHAILFYRQMLQWFGGMGIIVLAVAILPILGVGGMQL
                     YRAEMPGPLKDNKMRPRIAETAKTLWLIYVLLTVACALALWFAGMDAFDAIGHSFATI
                     AIGGFSTHDASIGYFDSPTINTIIAIFLLISGCNYGLHFSLLSGRSLKVYWRDPEFRM
                     FIGVQFTLVVICTLVLWFHNVYSSALMTINQAFFQVVSMATTAGFTTDSIARWPLFLP
                     VLLLCSAFIGGCAGSTGGGLKVIRILLLFKQGNRELKRLVHPNAVYSIKLGNRALPER
                     ILEAVWGFFSAYALVFIVSMLAIIATGVDDFSAFASVVATLNNLGPGLGVVADNFTSM
                     NPVAKWILIANMLFGRLEVFTLLVLFTPTFWRE"
     gene            33693..34238
                     /gene="hemG"
                     /locus_tag="OO357_001847"
     CDS             33693..34238
                     /gene="hemG"
                     /locus_tag="OO357_001847"
                     /EC_number="1.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="menaquinone-dependent protoporphyrinogen IX
                     dehydrogenase"
                     /protein_id="HCR5886813.1"
                     /translation="MKTLILFSTRDGQTREIASYLASELKELGIQTDVANVHRIEEPQ
                     WENYDRVVIGASIRYGHYHSAFQEFVKKHATRLNSMPSAFYSVNLVARKPEKRTPQTN
                     SYARKFLMNSQWRPDRCAVIAGALRYPRYRWYDRFMLKLIMKMSGGETDTRKEVVYTD
                     WEQVANFAREIAHLTDKPTLK"
BASE COUNT         8108 a         8699 c         9302 g         8338 t
ORIGIN      
        1 ccttctgctg accgttttcg atgtacaaac cgagcggcgc atagctttca tcatagatgc
       61 cgccgttcat cgccatctgc acctgaccct gactattaat atccgccagc agagcatgta
      121 acgttcccca cgcttcgcca ttggcttttt gccagtacat tttcacccgc tctgtttgag
      181 gattaacggt atacgcctgt acggtcagcg tcggatctga gagtgcgcaa tcatcagcgg
      241 caacagcaaa caagggaagt aaggtgaggg tgagaaaaat ccgtttgaga ttcaaggtga
      301 tcattccttt accaatgagt agccgatgcg ccattatagg tcctagatgt gggatttttt
      361 tatcctgtta gcgaccttga cgagtaccaa aaagcgcgaa gttcaactat tgttctgtgg
      421 tgttctgttg cgtgttgacg gcaaaatttt gctggcgtaa catgcgcgca cgatcactct
      481 aagaggacat tcgccttgga cacacccagt agatactggc tcactatcct gtcatccagg
      541 atcaactcct aaggctatcc ctttttgctg atagccttag cggttgtcag cgacctcaat
      601 ttttcccgtc gcgctgagtc aggctgttta atggtctgaa acccaatttg tttctgtgtg
      661 cccaccgaac tgtccgatat tttaagcatt gggagtcccg gtcatgctga gcgcatttca
      721 actggaaaat aaccgactga cccggctgga agtcgaagag tcacaacccc ttgtaaatgc
      781 agtatggatt gatcttgtcg aaccggacga cgacgagcga ctgcgcgtac aatctgaact
      841 tggccagagc ctggcaaccc gcccggaact ggaagacatc gaagcatcgg cacgtttctt
      901 tgaagacgac gacggcctgc atattcactc cttcttcttc tttgaagatg cggaagatca
      961 cgccggtaac tccactgtgg catttaccat ccgtgatggt cgtctgttta ctctgcgtga
     1021 gcgtgaactg cccgcttttc gtctgtatcg tatgcgtgcc cgtagccagt cgatggtaga
     1081 cggtaacgcc tacgagttgc tgctggatct gttcgaaacc aaaatcgaac agttggcaga
     1141 tgaaattgaa aatatctata gcgacctgga gcagttgagc cgggtgatta tggaagggca
     1201 tcagggcgat gagtacgacg aggcgctctc cacgctggcg gaactggaag atatcggctg
     1261 gaaagttcgc ctgtgtctga tggataccca gcgcgctctc aacttcctgg tgcgtaaagc
     1321 gcgtttaccg ggtgggcaac tggagcaggc gcgtgaaatc ctgcgcgata tcgaatccct
     1381 gctgccgcat aacgaatccc tgttccagaa ggtgaacttc ctgatgcagg cggcaatggg
     1441 ttttatcaac atcgagcaga accgcatcat caaaattttc tcggtggtat ccgtcgtgtt
     1501 cctgccgcca acgctggttg cttccagcta cgggatgaac tttgagttta tgccagaact
     1561 gaagtggagt ttcggctacc ctggcgcgat tatctttatg atcctcgcgg gcctggcacc
     1621 gtatctgtac tttaagcgga agaactggtt gtaaaaagaa ggcggtggct tagcctggct
     1681 aagccacctg ttattcaaag gctccaggta tttaaccctt ttacctcttt ctcataaaac
     1741 catttgttcg tgttaacagc aacataggct gctacggcaa ttcccagaat gttaacgcca
     1801 attaatgcac caacaaatag agatagaatg ccaagtaata gaactataat tgcttttttc
     1861 cataagccca gaacaaataa atagatccag gaacagaaga aagcaatgaa attcatttga
     1921 atagttaatc gttgtcttac ttttaatgct ttaaatgctg ctttatattc tggtgttgct
     1981 ccccagaatc caggaaagcc atgttgatca taaaaattaa atcggtattt ccatttttca
     2041 cttaatgaat catcgttcat atattcctta ctcataaata ctccataact attgttttga
     2101 tgaatcagta ggtgcaagca ttagcatact gaaagtggaa aaataacaaa tcaaaaaaat
     2161 catcgaacca ttgcctgaac aggcaaaatc ttcggctatc attgtgatga tagagatgat
     2221 atatactgct aatgtaccaa aaacataaat ttttatatat atgaaaccac tatcacggag
     2281 tcgttggcaa ttcatgttga tgacgagata atggagtgcg atggtagaga ctataacaag
     2341 aaaacctgct tccccatcgt taaaaaagat aataagaaag gcaaaaatga aatttattaa
     2401 aataaataaa aatatatatc ggtgtctgga aatcttaccg ttagatgttg ggataaacat
     2461 acgtaacata aattttacat ccttgtgtga gtctcctgtc agcatggcaa tatgcccact
     2521 ctcatacaag agtcggcata ttttttcaga atatatttat tttttatttg gacgttctac
     2581 gctgcgtata aatcgcgtcc atcacaaaaa ttgccagcgc cacccaaata aaggtgaaag
     2641 tcaccatctt atcggcaccc ggtttttcac cataaaacgt cacagccagc aggaacatca
     2701 gcgtcgggcc aatgtactga aaaaagccta acgttgagag acgcaggcgc gtggcagcgg
     2761 cagtaaaaca caacagcggt acggtagtga caataccggc ggcgatcagc agtaaattca
     2821 gcgacatcgg gttttgcccc atatggctgg ttgaactgtc ggcaatagca aacaggtaaa
     2881 tcgccgccac gggcagcagc cacatggttt caattaacat gccggtttgt gcttctaccg
     2941 caatcttctt gcgcaccagg ccgtagaagg caaaactaaa tgccagcccc agcgcgataa
     3001 taggtagcga accaaaagtc cacagctgga ccaacacgcc acatatcgcc agaatcaccg
     3061 ccagccattg catccggcgg aatcgctcgc cgaggaaaat catccccagc acaatgttca
     3121 ccagcgggtt aataaagtaa ccaaggctcg cttccagcat atggtgattg ttcaccgccc
     3181 agataaacag tagccagttg ccaccaatca gtacggcaga gactgccagc ataaaaattt
     3241 tctgtggcgt ctgaatcagc gtttttaaat aggaccactg gcggcaaatg ctcatcagca
     3301 ccaccataaa gaaaaacgac cagatcacgc gatgcgtcag gatctcatcg gcgggcacgt
     3361 agtaaatcag cttgaagtat gctggcgcaa taccccaaat aaaataagcg gcaagagcga
     3421 gtaatacgcc ctgccgcgtt tgttttgcat ccatcgggaa tactcatttt taattggtaa
     3481 cagcagttta cctgctttta tgtcttcaac ctaccatata ggtggcggtg gcactggcaa
     3541 tataaagctg ttcttcattg tgtaattcaa cgcgggcgac ggcgacttta ttgcctgcac
     3601 gcaacaggct actggtagca gtaaaacgct cgcccctgcc tgggcgcaga taatcaacgc
     3661 gaagatcaat ggtccccatc cgcgatagcc gctggcgtag ttcatcttca ctgatggttt
     3721 cgtggcgggt taaggtactt cccacgcaca ccagaccggc ggcgacatcc agcgccgacg
     3781 caatgactcc gccgtgcaaa atgctttgcg cccagttacc caccatcatc ggctgatttt
     3841 taaaggccag ctgtgcgaac tctttttcgt aacgctccag ttccatcccc aatgcgcggt
     3901 taaatggcat gtgataaaca aacatctcac ccactaattt cagggcttgt tcagcggtca
     3961 gtacggcaga catatcatcc ttacacttca ttggttaatg aaatgttgat tttatgcttc
     4021 tttgttgttg gtttctactt taggaaggga taactaacgg caatggagtt aagtatgtaa
     4081 aatagcccgc agaaaaacat tcaccttatc aataattcgt tacggagaac acgaccgatg
     4141 cggactctgc agggctggtt gttgccggtg tttatgttgc ctatggcagt atatgcacaa
     4201 gaggcaacgg tgaaagaggt gcatgacgcg ccagcggtgc gtggcagtat tatcgccaat
     4261 atgctgcagg agcatgacaa tccgttcacg ctctatcctt atgacaccaa ctacctcatc
     4321 tacacccaaa ccagcgatct gaataaagaa gcgattgcca gttacgactg ggcggaaaat
     4381 gcgcgtaagg atgaagtaaa gtttcagttg agcctggcat ttccgctgtg gcgtgggatt
     4441 ttaggcccaa actcggtgtt gggtgcgtct tatacgcaaa aatcctggtg gcaactgtcc
     4501 aatagcgaag agtcttcacc gtttcgtgaa accaactacg aaccgcaatt gttcctcggt
     4561 tttgccaccg attaccgttt tgcaggttgg acgctgcgcg atgtggagat ggggtataac
     4621 cacgactcta acgggcgttc agacccgacc tcccgcagct ggaaccgcct ttatactcgc
     4681 ctgatggcag aaaacggtaa ctggctggta gaagtgaagc cgtggtatgt ggtgggtaat
     4741 actgacgata acccggatat caccaaatat atgggttact accagcttaa aatcggctat
     4801 cacctcggtg atgcggtgct cagtgcgaaa ggacagtaca actggaacac cggctacggc
     4861 ggcgcggagt taggcttaag ttacccgatc accaaacatg tgcgccttta tactcaggtt
     4921 tacagcggct atggcgaatc gctcatcgac tataacttca accagacccg tgtcggtgta
     4981 ggggttatgc taaacgactt gttttgatga acggttgagt ggttggcaaa tctggaatcc
     5041 agcatccagg attaccctct cagagactaa aagcattgca gtttctcgcg caggcgctga
     5101 aaatagcgcc tgtttttatt tcaggcaatc ggggtgaatg tggcgcaggc ggaagtgttg
     5161 aatctggagt ccggagctaa acaggtttta caagaaacct ttggctacca acagtttcgc
     5221 cccggccagg aagaaattat cgacactgtg ctttccggtc gcgattgcct ggttgttatg
     5281 cccactggtg gcggaaaatc cctttgctat caaattcctg ccttattgct aaacggcctt
     5341 accgtggttg tttcaccgct gatttcgttg atgaaagatc aggtggatca actacaggcc
     5401 aacggcgtgg cggcggcgtg ccttaactcg acgcaaaccc gcgaacagca acttgaagtg
     5461 atgacaggct gccgcaccgg gcaaattcgc ttactgtata tcgccccgga acgcctgatg
     5521 ctggataact ttcttgagca tctggcgcac tggaatccgg tgttattagc cgtcgatgaa
     5581 gcgcactgta tctcccaatg gggtcacgat ttccgcccgg aatatgccgc gctcggtcag
     5641 ttgcgccagc ggttcccgac gctgccgttt atggcgctga ccgccacagc cgacgacacc
     5701 acgcgccagg atatcgtgcg cctgctgggg ctgaacgatc cgctgattca aatcagcagt
     5761 tttgaccgtc cgaatattcg ctacatgctg atggagaaat tcaaaccgct cgatcagttg
     5821 atgcgctacg tgcaggaaca gcgcggtaag tctggcatta tctactgcaa cagccgggcg
     5881 aaagtagaag acaccgctgc gcgcctgcaa agcaggggta ttagcgcggc ggcctatcat
     5941 gccgggctgg aaaataatgt ccgcgccgac gtgcaggaga aattccagcg cgatgatctg
     6001 caaattgtgg tggcgacggt ggcgttcggc atgggcatca ataaaccaaa cgttcgcttc
     6061 gtggtccact ttgatattcc gcgcaatatc gaatcctatt atcaggaaac cggtcgcgcc
     6121 gggcgtgatg gcctgcctgc ggaagcgatg ctgttttacg atccggctga tatggcgtgg
     6181 ctgcgccgtt gtctggaaga gaagccgcag gggcagttgc aggatatcga gcgccacaaa
     6241 ctcaatgcga tgggcgcgtt tgccgaagcg caaacttgcc gtcgtctggt attgctgaac
     6301 tattttggcg aagggcgtca ggagccgtgc gggaactgcg atatctgcct cgatccgccg
     6361 aaacagtacg acggttcaac cgatgctcag attgcccttt ccaccattgg tcgtgtgaat
     6421 cagcggtttg ggatgggtta tgtggtggaa gtgattcgtg gtgctaataa ccagcgtatc
     6481 cgcgactatg gtcatgacaa actgaaagtc tatggtatgg gccgtgataa aagccatgaa
     6541 cattgggtga gcgtgatccg ccagctgatt catctcggcc tggtgacgca aaatattgcc
     6601 cagcattctg ccctacaact gacagaggcc gcgcgcccgg tgctgcgcgg cgaatcctct
     6661 ttgcaacttg ccgtgccgcg tatcgtggcg ctcaaaccga aagcgatgca gaaatcgttc
     6721 ggcggcaact atgatcgcaa actgttcgcc aaattacgca aactgcgtaa atcgattgcc
     6781 gatgaaagta atgtcccgcc gtacgtggtg tttaacgacg caaccttgat tgagatggct
     6841 gaacagatgc cgatcaccgc cagcgaaatg ctcagcgtta acggcgttgg gatgcgcaag
     6901 ctggaacgct ttggtaaacc gtttatggcg ctgattcgtg cgcatgtcga tggcgacgac
     6961 gaagagtagt cagcagcata aaaaagtgcc agtatgaaga ctccgtaaac gtttcccccg
     7021 cgagtcaaat gtatgttgat gttatttctc accgtcgcca tggtgcacat tgtggcgctt
     7081 atgagccccg gtcccgattt cttttttgtc tctcagaccg ctgtcagtcg ttcccgtaaa
     7141 gaagcgatga tgggcgtgct gggcattacc tgcggcgtaa tggtttgggc tgggattgcg
     7201 ttgcttggcc tgcatttgat tatcgaaaaa atggcctggc tgcatacgct gattatggta
     7261 ggcggtggcc tgtatctctg ctggatgggt taccagatgc tacgcggtgc actgaaaaaa
     7321 gaggctgtat ctgcgcctgc accacaggtt gagctggcga aaagcgggcg cagtttcctg
     7381 aaaggcttac tgaccaatct cgccaatccg aaagcgatta tctattttgg ttcggtattc
     7441 tcattgtttg tcggcgataa cgttggcact accgagcgct ggggcatttt cgcgttgatt
     7501 attgtcgaaa cactggcgtg gtttaccgtc gttgccagtc tgtttgccct gccgcaaatg
     7561 cgccgtggtt atcaacgtct ggcgaagtgg attgatggtt ttgccggggc gttatttgcc
     7621 ggatttggca ttcatttgat tatttcgcgg tgatgccaga cgcgtcttca gagtaagtcg
     7681 gataaggcgt ttacgccgca tccgacatta tttttcacgc atgcctcgcc gatgctaaca
     7741 gcgctcccac cagcataaac aacgagccga aaatcttatt cagcgccttc atctgctttg
     7801 gtcctttaat ccatagagca atcctttgag caagggtggc gtaaccgatc atcacaataa
     7861 tatcgaccac aatagtggtg acgccgagca cgatatactg catcagttgc ggctgttgcg
     7921 gcatgatgaa ttgcggaaat agcgccgcca gaaacacaat acttttggga ttggtgagat
     7981 tcacaaaaac tgcgcgctgg aacaaatgtc gacgcgattg agtagaggcc agcgatttaa
     8041 gatcaattgc gccagcggcg cgccactgct ggattcccag ccaaatcaag taagccgcgc
     8101 ctgcccactt taacacttca aacgcaatca ctgagcggga aaatagcgtc cccaacccca
     8161 cgccaaccag cacaatatga atcgccagtc cggtctgaag cccagcaata gacgccaccg
     8221 cgccgcgata accgtggttg agcgaggtgg tcatagtgtt gattgcacca gagcctggcg
     8281 acaggcttaa aatgatcgat gtcagcaggt aggcaaacca ccattctaag gtcatgatga
     8341 actcccggtg tgtctatttt tgtgccacaa tacgctactg tcgcagcgtt gtgtcaggca
     8401 cgcttaaaaa aacgatttta cgtggtttaa gaggcagatt acccgatgtt tcagcagcaa
     8461 aaagactggg aaacaagaga aaacgcgttt gctgctttta ccatgggacc gctgactgat
     8521 ttctggcgtc agcgtgatga agcagagttt actggtgtgg atgacattcc ggtgcgcttt
     8581 gtccgttttc gcgcacagca ccatgaccgg gtggtagtca tctgcccggg gcgtattgag
     8641 agctacgtaa aatatgcgga actggcctac gacctgttcc atttggggtt tgatgtctta
     8701 atcatcgacc atcgcgggca gggacgttcc ggtcgcctgt tagccgatcc gcatctcggg
     8761 catgttaatc gctttaatga ttatgttgat gatctggcgg cattctggca gcaggaggtt
     8821 cagcccggtc cgtggcgtaa acgctatata ctggcacatt cgatgggcgg tgcgatctcc
     8881 acattatttc tgcaacgcca tccaggtgta tgtgacgcca ttgcgctaac tgcgccaatg
     8941 tttgggatcg tgattcgtat gccgtcattt atggcacggc agatcctcaa ctgggccgaa
     9001 gcgcatccac gtttccgtga tggctatgca ataggcaccg ggcgctggcg cgcgttgccg
     9061 tttgctatca acgtactgac ccacagcaga cagcgatatc gacgtaactt acgcttctat
     9121 gctgatgacc caacgattcg cgtcggtggg ccgacctacc attgggtacg cgaaagtatt
     9181 ctggctggcg aacaggtgtt agccggtgcg ggtgatgacg ccacgcaaac gcttctctta
     9241 caagctgaag aggaacgcgt ggtggataac cgcatgcacg accgtttttg tgaactccgc
     9301 accgccgcgg gccatcctgt cgaaggagga cggccgttgg taataaaagg tgcttaccat
     9361 gagatccttt ttgaaaagga cgcaatgcgc tcagtcgcgc tccacgccat cgttgatttt
     9421 ttcaacaggc ataactcacc cagcggaaac cgctctacag aggtttaaat ttcttatgta
     9481 ccaggttgtt gcgtctgatt tagatggcac gttactttct cccgaccata cgttatcccc
     9541 ttacgccaaa gaaactctga agctgctcac cgcgcgcggc atcaactttg tgtttgcgac
     9601 cggtcgtcac cacgttgatg tggggcaaat tcgcgataat ctggagatta agtcttacat
     9661 gattacctcc aatggtgcgc gcgttcacga tctggatggc aatctgattt ttgctcataa
     9721 cctggatcgc gacattgcca gcgatctgtt tggcgtagtc aacgacaatc cggacatcat
     9781 tactaacgtt tatcgcgacg acgaatggtt tatgaatcgc catcgcccgg aagagatgcg
     9841 cttttttaaa gaagcggtgt tcaaatatgc gctgtatgag ccgggattac tggagccgga
     9901 aggcatcagc aaagtgtttt tcacctgcga ttcccatgaa caactgctgc cgctggagca
     9961 ggcgattaac gctcgctggg gcgatcgcgt caacgtcagt ttctctacct taacctgtct
    10021 ggaagtgatg gcgggcggcg tgtcgaaagg ccatgcgctg gaagcagtgg cgaagaaact
    10081 gggctacagc ctgaaggatt gtattgcgtt tggtgacggg atgaacgacg ccgaaatgct
    10141 gtcgatggcg gggaaaggct gcattatggg cagtgcgcac cagcgtctga aagatctgca
    10201 tccggagctg gaagttattg gtactaatgc cgacgacgcg gtgccgcatt atctgcgtaa
    10261 actctattta tcgtaatcgt tctttatttg gtcagttgtc aacctgatac ttcgctacaa
    10321 tggatactcg ttaatcaaag agttttccat tgtggcgtta cttatcatca ccacgattct
    10381 gtgggccttc tcctttagct tttatggcga gtaccttgcg gggcacgtcg atagctattt
    10441 tgcggtgctg gtgcgcgttg gcttggcggc actcgttttt ctgccgtttc tgcgtacccg
    10501 tagcaatagc ctgaaaacgg tcggcctgta tatgctggtg ggcgcgatgc agctcggcgt
    10561 gatgtatatg ctgagtttcc gcgcttatct ctacctgacg gtctccgaat tgttgctgtt
    10621 caccgtactg acgccgctct acatcacgct gatttatgac atcatgagta agcgccgtct
    10681 gcgctggggc tatgctttta gcgccttgct ggcggtgatt ggtgccggga ttattcgcta
    10741 tgatcaggtc accgaccatt tctggactgg cttgttgctg gtgcaactct ccaatatcac
    10801 ttttgccatc ggcatggtgg gttacaaacg cctgatggaa actcgcccga tgccgcagta
    10861 taacgccttt gcgtggttct atcttggcgc gttcctggtg gcggtaattg catggttctt
    10921 gctgggaaat gcgcagaaaa tgccgcaaac cacgttgcaa tggggcattc tggtgtttct
    10981 tggcgtggtg gcttccggga ttggctactt tatgtggaac tacggcgcga cgcaggtgga
    11041 cgccggaacg ctgggcatta tgaataatat gcacgttccg gcagggctgc tggtaaacct
    11101 ggctatctgg caccaacagc cgcactggcc aacgtttatt acaggcgcgc tggtgatcct
    11161 ggcctcactg tgggtgcatc gtaagtgggt cgctccgcgc tcttcacaaa cggcagatga
    11221 tcgcaggcgt gattgcgcgc tgagcgaata aacgcttccg taactggctg acgctgctcg
    11281 ccatcgcgca cggcggcgta cagtcggctc cacaagcctt cgcccagggt tttggtcacc
    11341 accagaccct ggcgctcaaa actctctact acccaatgcg gtagcgcggc aatacccatc
    11401 cgcgcggcaa ccatttgaat caacaataag gtgttatcga cgcttttcag tgacgggctg
    11461 acgcctgccg gctgaagaaa atgccgccag acatccagtc gactacgctg caccggataa
    11521 attaatagcg tctcgctggc gagatcttcc ggtgtaatac gcgtttttgc cgccagtgga
    11581 tggtcaggtg ctaacaccag gcgtacttca taatcgaaca tcggcgaata atgcaggcca
    11641 ctgcgcggca ggatatcgga ggtcatcacc agatccagct cgccctgttg caacgcgggc
    11701 tgcgggtcaa acgtcacgcc cgatttaaaa tccatctcta cctgcggcca gttcttatgg
    11761 aaatttccta aagcgggtgt cagccactga atacagctat ggcactcaat ggcaatgcgc
    11821 agacgcgtct gctgcggttc attgcaggct tgcagtgcct ggctgatttg cggcagcacc
    11881 tggtttgcca gttgcaacag gatttctccc tgcggtgtaa agcgtagcgg ctggctctta
    11941 cgcacaaata gccggaagcc aaggcgttgt tccagatcgc taaactggtg agacagggcg
    12001 gattgcgtct gatgcaacgt tgccgcagcg gctgcgagcg agccgcagtt ccgcaacgct
    12061 tgtagcgttt tcaggtgttt tacttcgatc atgaaagtcc ttcacttcgg catgaataat
    12121 ttgcgcttga ggaatataca gtaaccgcca attatggatg tgtaaacatc tggacggcta
    12181 aaatcattcg tcttttaaat ttatggtgcg ttggctgcgt ttctccaccc cggtcactta
    12241 cttcagtaag ctcccggcga tgaataaact tgccgccttc cctaaattca aaatccatag
    12301 gatttacata taattagagg aagaaaaatg acaatattga atcacaccct cggtttccct
    12361 cgcgttggcc tgcgtcgcga gctgaaaaaa gcgcaagaaa gttattgggc ggggaactcc
    12421 acgcgtgaag aactgctgac agtagggcgt gaactgcgtg ctcgtcactg ggatcaacaa
    12481 aagcaagcgg gtatcgacct gctgccggtg ggcgattttg cctggtacga tcatgtactg
    12541 accaccagtt tgctgctggg taatgttccg ccacgtcatc agaacaaaga cggttcggta
    12601 gatatcgaca ccctgttccg tattggtcgt ggacgtgcgc cgactggcga acctgcggcg
    12661 gcagcggaaa tgaccaaatg gtttaacacc aactatcact acatggtgcc ggagttcgtt
    12721 aaaggccaac agttcaaact gacttggacg cagctgctgg aggaagtgga cgaggcgctg
    12781 gcgctgagcc acaacgttaa gcctgtgctg ctggggccgg ttacttacct gtggctgggg
    12841 aaagtgaaag gtgaacagtt tgaccgcctt agcctgctga acgacattct gccggtttat
    12901 cagcaagtgc tggcagaact ggcgaaacgc ggcatcgagt gggtacagat tgatgaaccc
    12961 gcgctggtac tggaactacc acaggcgtgg ctggacgcat acaaacccgc ttacgacgcg
    13021 ctccagggac aggtgaaact gctgctgacc acctattttg aaggcgtaac gccaaatctc
    13081 gacacgatta ctgcgctgcc tgttcagggg ctgcatgtgg accttgtaca tggtaaagat
    13141 aacgttgttg aactgcacaa gcgcctgcct tctgactggc tgctgtctgc gggtctgatc
    13201 aatggtcgta acgtctggcg cgccgatctt actgagaaat atgcgcaaat taaggacatt
    13261 gtcggcaaac gtgatttgtg ggtggcatct tcctgctcgt tgctgcacag ccccatcgac
    13321 ctgagcgtgg aaacgcgtct tgatgcagaa gtgaaaagct ggtttgcctt cgccctacaa
    13381 aaatgccatg aactggcact gctgcgcgat gcgctgaaca gtggtgacac ggcagctctg
    13441 gcagagtgga gcgccccaat tcaggcgcgt cgtcactcta ctcgtgtaca taatccggcg
    13501 gtagaaaagc gtctggcggc gatcaccgcc caggacagcc agcgtgcgaa tgtctatgaa
    13561 gtgcgtgctg aagcccagcg tgcgcgtttt aaactgccag cgtggccgac caccacgatt
    13621 ggttccttcc cgcaaaccac ggaaattcgt accctgcgtc tggatttcaa aaagggcaat
    13681 ctcgacgcca acaactaccg cacgggcatt gcggaacata tcaagcaggc cattgttgag
    13741 caggaacgtt tgggactgga tgtgctggta catggcgagg ccgagcgtaa tgacatggtg
    13801 gaatactttg gcgagcacct cgacggattt gtctttacgc aaaacggttg ggtacagagc
    13861 tacggttccc gctgcgtgaa gccaccgatt gtcattggtg acattagccg cccggcaccg
    13921 attaccgtgg agtgggcgaa gtatgcgcaa tcgctgaccg acaaaccggt gaaagggatg
    13981 ctgacggggc cggtgaccat actctgctgg tcgttcccgc gtgaagatgt cagccgtgaa
    14041 accatcgcca aacagattgc gctggcgctg cgtgatgaag tggccgatct ggaagccgct
    14101 ggaattggca tcatccagat tgacgaaccg gcgctgcgcg aaggtttacc gctgcgtcgt
    14161 agcgactggg atgcgtatct ccagtggggc gtagaggcct tccgtatcaa cgccgccgtg
    14221 gcgaaagatg acacacaaat ccacactcac atgtgttatt gcgagttcaa cgacatcatg
    14281 gattcgattg cggcgctgga cgcagacgtc atcaccatcg aaacctcgcg ttccgacatg
    14341 gagttgctgg agtcgtttga agagtttgat tatccaaatg aaatcggtcc tggcgtctat
    14401 gacattcact cgccaaacgt accgagcgtg gaatggattg aagccttgct gaagaaagcg
    14461 gcaaaacgca ttccggcaga gcgcctgtgg gtcaacccgg actgtggcct gaaaacgcgc
    14521 ggctggccag aaacccgcgc ggcactggcg aacatggtgc aggcggcgca gaatttgcgt
    14581 cgggggtaaa tccaaaccgg gtggtaatac cacccggtct tttctcatta cagcgacttc
    14641 ttcccaccat actgcttaaa ccattccagc atacgctgcc agccatcttt cgcagattcg
    14701 gcatgatagc tcgggcgata atcagcgttg aatgcatgcc cggcatccgg gtacacgata
    14761 atctctgctt tcgcattagc agcccgcagc gcctggcgca tcgtttcaac gctctcctgc
    14821 ggaatgctgt tatcctgacc accatataag ccgagaaccg gcgcgttaag atcggttgcg
    14881 atatcaacag gttgtttcgg tgaattcagc gacttgtcgc ccgtcagttt gccgtaccac
    14941 gccactgcgg cttttagctg tggattatgc gcggcataca gccaggtgat acgtccaccc
    15001 cagcagaatc cggtgatcat taaacgatga acatcgccgc cgttgcggga agcccaactg
    15061 gcgacatgat cgagatcggc cagcacctgc gagtcaggca cttttgctac cagaccgcta
    15121 agcaacgtgg ggatatcggc aaaatcattc ggatcgcctt cgcggaagta aagttcaggt
    15181 gcgatagcca gatacccctc cagcgccaga cggcgacaaa tgtcgcggat atgttcatgc
    15241 acgccaaaaa tttcctgcac tacaatgacc actggcagtg ggccatcgct ttgctttggt
    15301 ctggcatggt aagcaggcat gttatcccct tgtgaaggga tagaggtgaa gcccgccaca
    15361 atcgcgtcgt ccggggtctg aacgatggtc gaagcgagag gcgatgcagc aggtgcaaat
    15421 ccagattgtt gtgttgttgc catggtattc tccgtaccct tataaaaatg ttgcgcaatg
    15481 ttaactatag tcagcatgca acaaatcaca ttgcctgaat cggctcatct tttatgcagt
    15541 cctgcagaat gaagagtgat ttatgtgata tgaatcactt ttggtgggta aatttatgca
    15601 acgcatttgc gtcatggtga tgagtatcac gaaaaaatgt taaacccttc ggtaaagtgt
    15661 ctttttgctt cttctgacta aaccgattca cagaggagtt gtatatgtcc aagtctgatg
    15721 tttttcatct cggcctcact aaaaacgatt tacaaggggc tacgcttgcc atcgtccctg
    15781 gcgacccgga tcgtgtggaa aagatcgccg cgctgatgga taagccggtt aagctggcat
    15841 ctcaccgcga attcactacc tggcgtgcag agctggatgg taaacctgtt atcgtctgct
    15901 ctaccggtat cggcggcccg tctacctcta ttgctgttga agagctggca cagctgggca
    15961 ttcgcacctt cctgcgtatc ggtacaacgg gcgctattca gccgcatatt aatgtgggtg
    16021 atgtcctggt taccacggcg tctgtccgtc tggatggcgc gagcctgcac ttcgcaccgc
    16081 tggaattccc ggctgtcgct gatttcgaat gtacgactgc gctggttgaa gctgcgaaat
    16141 ctattggcgc gacaactcac gttggcgtga cagtttcttc tgataccttc tacccaggtc
    16201 aggaacgtta cgatacttac tctggtcgcg tagttcgtca ctttaaaggt tctatggaag
    16261 agtggcaggc gatgggcgta atgaactatg aaatggaatc tgcaactctg ctgaccatgt
    16321 gtgcaagtca gggcctgcgt gccggtatgg tagcgggtgt tatcgttaac cgcacccagc
    16381 aagagatccc gaatgctgag acgatgaaac aaaccgaaag ccatgcggtg aaaatcgtgg
    16441 tggaagcggc gcgtcgtctg ctgtaattct cttctcctgt ctgaaggccg acgcgttcgg
    16501 ccttttgtat ttttgcgtag cgcctcgcag gaaatgcctt tccaactgga cgtttgtaca
    16561 gcacaattct attttgtgcg ggtaagttgt tgcgtcagga ggcgttgtgg atttctcaat
    16621 catggtttac gcagttattg cgttggtggg tgtggcaatt ggctggctgt ttgccagtta
    16681 tcaacatgcg cagcaaaaag ccgagcaatt agctgaacgt gaagagatgg tcgcggagtt
    16741 aagcgcggca aaacaacaaa ttacccaaag cgagcactgg cgtgcagagt gtgaattgct
    16801 caataacgaa gtgcgcagcc tgcaaagtat taacacctct ctggaggccg atctgcgtga
    16861 agtaaccacg cggatggaag ccgcacagca acatgctgac gataaaattc gccagatgat
    16921 taacagcgag cagcgcctca gtgagcagtt tgaaaacctc gccaaccgta tttttgagca
    16981 cagcaatcgc cgggttgatg agcaaaaccg tcagagtctg aacagcctgt tgtcgccgct
    17041 acgtgaacaa ctggacggtt tccgccgtca ggttcaggac agcttcggta aagaagcaca
    17101 agaacgccat accctgaccc acgaaattcg caatctccag caactcaacg cgcaaatggc
    17161 ccaggaagcg atcaacctga cgcgcgcgct gaaaggcgac aataaaaccc agggcaactg
    17221 gggcgaggta gtattgacgc gggtgctgga ggcttccggt ctgcgtgaag ggtatgaata
    17281 tgaaacccag gtcagcatcg aaaatgacgc ccgctcgcgg atgcagccgg atgtcatcgt
    17341 gcggctgccg cagggaaaag atgtggtgat cgacgccaaa atgacgctgg tcgcctatga
    17401 acgctatttt aacgccgaag acgactacac ccgcgaaagc gcgctacagg aacatatcgc
    17461 gtcggtgcgt aaccatatcc gtttgctggg acgcaaagat tatcaacagc tgccggggct
    17521 gcgaactctg gattacgtgc tgatgtttat tcccgttgaa cccgcttttt tactggcgct
    17581 tgaccgccag ccggagctga tcaccgaagc gttgaaaaac aatatcatgc tggttagccc
    17641 gactacgctg ctggtggcgt tgcgtactat tgccaacctg tggcgttatg agcatcaaag
    17701 ccgtaacgcc cagcaaatcg ccgatcgtgc cagcaagctg tacgacaaga tgcgtttgtt
    17761 catcgatgac atgtccgcga ttggtcaaag tctcgacaaa gcgcaggata actaccgcca
    17821 ggcaatgaaa aaactctctt caggtcgcgg aaatgtgctg gcgcaggcag aagcgtttcg
    17881 cggtttagga gtagaaatta aacgcgagat taatccggat ttggctgaac aggcggtgag
    17941 ccaggatgaa gagtatcgac ttcggtcggt tccggagcag ccgaatgatg aagcttatca
    18001 acgcgatgat gaatataatc agcagtcgcg ctagcccatt aggggtagtt aagccgggta
    18061 gaaatctagg gcatcgacgc ccaatctgtt acacttctgg aacaattttt tgatgagcag
    18121 gcattgagat ggtggataag tcacaagaaa cgacgcactt tggttttcag accgtcgcga
    18181 aggaacaaaa agcggatatg gtcgcccacg ttttccattc cgtggcatca aaatacgatg
    18241 tcatgaatga tttgatgtca tttggtattc atcgtttgtg gaagcgattc acgattgatt
    18301 gcagcggcgt acgccgtggg cagaccgtgc tggatctggc tggtggcacc ggcgacctga
    18361 cagcgaaatt ctcccgcctg gtcggagaaa ctggcaaagt ggtccttgct gatatcaatg
    18421 aatccatgct caaaatggga cgcgagaagc tgcgtaatat cggtgtgatt ggcaacgttg
    18481 agtatgttca ggcgaacgct gaggcgctgc cgttcccgga taacaccttt gattgcatca
    18541 ccatttcgtt tggtctgcgt aacgtcaccg acaaagataa agcactgcgt tcaatgtatc
    18601 gcgtgctgaa acccggcggc cgcctgctgg tgcttgagtt ctcgaagcca attatcgagc
    18661 cgctgagcaa agcctatgat gcatactcct tccatgtgct gccgcgtatt ggctcactgg
    18721 tcgcgaacga cgccgacagc taccgttatc tggcagaatc catccgtatg catcccgatc
    18781 aggataccct gaaagccatg atgcaggatg ccggattcga aagtgtcgac tactacaatc
    18841 tgacggcagg ggttgtggcg ctgcatcgtg gttataagtt ctgacaggag accggaaatg
    18901 ccttttaaac ctttagtgac ggcaggaatt gaaagtctgc tcaacacctt cctgtatcgc
    18961 tcacccgcgc tgaaaacagc ccgctcgcgt ctgctgggta aagtattgcg cgtggaggta
    19021 aaaggctttt cgacgtcatt gattctggtg ttcagcgaac gccaggttga tgtactgggc
    19081 gaatgggcag gcgatgctga ctgcaccgtt atcgcctacg ccagtgtgtt gccgaaactt
    19141 cgcgatcgcc agcagcttac cgcactgatt cgcagtggtg agctggaagt gcagggcgat
    19201 attcaggtgg tgcaaaactt cgttgcgctg gcagatctgg cagagttcga ccctgcggaa
    19261 ctgctggccc cttataccgg tgatatcgcc gctgaaggaa tcagcaaagc catgcgcgga
    19321 ggcgcaaagt tcctgcatca cggcattaag cgccagcaac gttatgtggc ggaagccatt
    19381 actgaagagt ggcgtatggc acccggtccg cttgaagtgg cctggtttgc ggaagagacg
    19441 gctgccgtcg agcgtgctgt tgatgccctg accaaacggc tggaaaaact ggaggctaaa
    19501 tgacgccagg tgaagtacgg cgcctatatt tcatcattcg cactttttta agctacggac
    19561 ttgatgaact gatccccaaa atgcgtatca ccctgccgct acggctatgg cgatactcat
    19621 tattctggat gccaaatcgg cataaagaca aacttttagg tgagcgacta cgactggccc
    19681 tgcaagaact ggggccggtt tggatcaagt tcgggcaaat gttatcaacc cgccgcgatc
    19741 tttttccacc gcatattgcc gatcagctgg cgttattgca ggacaaagtt gctccgtttg
    19801 atggcaagct ggcgaagcag cagattgaag ctgcaatggg cggcttgccg gtagaagcgt
    19861 ggtttgacga ttttgaaatc aagccgctgg cttctgcttc tatcgcccag gttcataccg
    19921 cgcgattgaa atcgaatggt aaagaggtgg tgattaaagt catccgcccg gatattttgc
    19981 cggtcattaa agcggatctg aaacttatct accgtctggc tcgctgggtg ccgcgtttgc
    20041 tgccggatgg tcgccgtctg cgcccaaccg aagtggtgcg cgagtacgaa aagaccttga
    20101 ttgatgaact gaatttgctg cgggaatctg ccaacgccat tcagcttcgg cgcaattttg
    20161 aagacagccc gatgctctac atcccggaag tttaccctga ctattgtagt gaagggatga
    20221 tggtgatgga gcgcatttac ggcattccgg tgtctgatgt tgcggcgctg gagaaaaacg
    20281 gcactaacat gaaattgctg gcggaacgcg gcgtgcaggt gttcttcact caggtctttc
    20341 gcgacagctt tttccatgcc gatatgcacc ctggcaacat cttcgtaagc tatgaacacc
    20401 cggaaaaccc gaaatatatc ggcattgatt gcgggattgt tggctcgcta aacaaagaag
    20461 ataaacgcta tctggcagaa aactttatcg ccttctttaa tcgcgactat cgcaaagtgg
    20521 cagagctaca cgtcgattct ggctgggtgc caccagatac caacgttgaa gagttcgaat
    20581 ttgccattcg tacggtctgt gaacctatct ttgagaaacc gctggccgaa atttcgtttg
    20641 gacatgtact gttaaatctg tttaatacgg cgcgtcgctt caatatggaa gtgcagccgc
    20701 aactggtgtt actccagaaa accctgctct atgtcgaagg ggtaggacgc cagctttatc
    20761 cgcagctcga tttatggaaa acagcgaagc ctttcctgga gtcgtggatt aaagatcagg
    20821 tcggtattcc tgctctggtg agagcattta aagaaaaagc gccgttctgg gtcgaaaaaa
    20881 tgccagaact gcctgaactg gtttacgaca gtttgcgcca gggcaagtat ttacagcaca
    20941 gtgttgataa gattgcccgc gagcttcagt caaatcatgt acgtcaggga caatcgcgtt
    21001 attttctcgg aattggcgct acgttagtat taagtggcac attcttgttg gtcagccgac
    21061 ctgagtgggg gctgatgccc ggctggttaa tggcaggtgg tctgatcgcc tggtttgtcg
    21121 gttggcgcaa aacacgctga ttttttcatc gctcaaggcg ggccgtgtaa cgtataatgc
    21181 ggctttgttt aatcatcatc taccacagag gaacatgtat gggtggtatc agtatttggc
    21241 agttattgat tattgccgtc atcgttgtac tgctttttgg caccaaaaag ctcggctcca
    21301 tcggttccga tcttggtgcg tcgatcaaag gctttaaaaa agcaatgagc gatgatgaac
    21361 caaagcagga taaaaccagt caggatgctg attttactgc gaaaactatc gccgataagc
    21421 aggcggatac gaatcaggaa caggctaaaa cagaagacgc gaagcgccac gataaagagc
    21481 aggtgtaatc cgtgtttgat atcggtttta gcgaactgct attggtgttc atcatcggcc
    21541 tcgtcgttct ggggccgcaa cgactgcctg tggcggtaaa aacggtagcg ggctggattc
    21601 gcgcgttgcg ttcactggcg acaacggtgc agaacgaact gacccaggag ttaaaactcc
    21661 aggagtttca ggacagtctg aaaaaggttg aaaaggcgag cctcactaac ctgacgcccg
    21721 aactgaaagc gtcgatggat gaactacgcc aggccgcgga gtcgatgaag cgttcctacg
    21781 ttgcaaacga tcctgaaaag gcgagcgatg aagcgcacac catccataac ccggtggtga
    21841 aagataatga agctgcgcat gagggcgtaa cgcctgccgc tgcacaaacg caggccagtt
    21901 cgccggaaca gaagccagaa accacgccag agccggtggt aaaacctgct gcggacgctg
    21961 aaccgaaaac cgctgcacct tccccttcgt cgagtgataa accgtaaaca tgtctgtaga
    22021 agatactcaa ccgcttatca cgcatctgat tgagctgcgt aagcgtctgc tgaactgcat
    22081 tatcgcggtg atcgtgatat tcctgtgtct ggtctatttc gccaatgaca tctatcactt
    22141 ggtatccgcg ccattgatca agcagttgcc gcaaggttca acgatgatcg ccaccgacgt
    22201 ggcctcgccg ttctttacgc cgatcaagct gacctttatg gtgtcgctga ttctgtcagc
    22261 gccggtgatt ctctatcagg tgtgggcatt tatcgcccca gcgctgtata agcatgaacg
    22321 tcgcctggtg gtgccgctgc tggtttccag ctctctgctg ttttatatcg gcatggcatt
    22381 cgcctacttt gtggtctttc cgctggcatt tggcttcctt gccaataccg cgccggaagg
    22441 ggtgcaggta tccaccgaca tcgccagcta tttaagcttc gttatggcgc tgtttatggc
    22501 gtttggtgtc tcctttgaag tgccggtggc aattgtgctg ctgtgctgga tggggattac
    22561 ctcgtcagaa gacttacgca aaaaacgccc gtatgtgctg gttggtgcat tcgttgtcgg
    22621 gatgttgctg acgccgccgg atgtcttctc gcaaacgcta ttggcgatcc cgatgtactg
    22681 tctgtttgaa atcggtgtct tcttctcacg cttttacgtt ggtaaagggc gaaatcggga
    22741 agaggaaaac gacgctgaag cagaaagcga aaaaactgaa gaataaattc aaccgcccgt
    22801 cagggcggtt gtcatatgga gtacaggatg tttgatatcg gcgttaattt gaccagttcg
    22861 caatttgcga aagaccgtga tgatgttgta gcgcgcgctt ttgacgcggg agttaatggg
    22921 ctactcatca ccggcactaa cctgcgtgaa agccagcagg cgcaaaagct ggcgcgtcag
    22981 tattcgtcct gttggtcaac ggcgggcgta catcctcacg acagcagcca gtggcaagct
    23041 gcgactgaag aagcgattat tgagctggcc gcgcagccag aagtggtggc gattggtgaa
    23101 tgtggtctcg actttaaccg taacttttcg acgccggaag agcaggaacg cgcttttgtt
    23161 gctcagctac gcattgccgc agaattaaac atgccggtat ttatgcactg tcgcgatgcc
    23221 cacgagcggt ttatgacatt gctggagccg tggctggata aactgcctgg tgcggttctt
    23281 cattgcttta ccggcacacg cgaagagatg caggcgtgcg tggcgcgtgg aatttatatc
    23341 ggcattaccg gttgggtttg cgatgaacga cgcgggctgg agctgcggga attgttgccg
    23401 ttgattccgg cggagaaatt gctgatcgaa acagatgcgc cgtatctgct cccgcgcgat
    23461 ctcacgccaa agccatcatc ccggcgcaac gagccagccc atctgcccca tattttgcaa
    23521 cgtattgcgc actggcgtgg agaagatgcc gcatggctgg ctgccaccac ggatgccaat
    23581 gtcaaaacac tgtttgggat tgcgttttag agtttgcgga actcggtgtt cttcacactg
    23641 tgcttaatct ctttattaat aagattaagc aatagcatgg agcgagcctc accatcgggt
    23701 tcggtgaaaa tggcctgaaa gccttcgaac gcgccttcgg taataatcac cttatcaccc
    23761 ggataagggg gggccggatc gacaatgtct ttcggtttat ataccgatag ctgatgaatt
    23821 accgccgatg ggactatcgc tggcgacgcg ccaaagcgca caaagtggct gacgccacgc
    23881 gtagcgctga tggtcgtggt atgaatcact tctggatcaa attccacaaa caggtagtta
    23941 gggaataacg gttcactgac tgcggtacgt ttaccccgta cgattttttc cagggtgatc
    24001 atcggagcga ggcaattaac cgcctgtctc tcaaggtgtt cctgggcacg ttgaagttgc
    24061 ccgcgcttgc agtacagtaa ataccaggat tgcataatga ctcttatccg tttaatcggg
    24121 gcgcaaggat agcaaaagct ttacgctaag ttaattatat tccccggttt gcgttatacc
    24181 gtcagagttc acgctaattt aacaaattta cagcattgca aagatgaacg ccgtataatg
    24241 ggcgcagatt aagaggctac aatggacgcc atgaaatata acgatttacg cgacttcctg
    24301 acgctgcttg aacagcaggg tgagctaaaa cgtatcacgc tcccggtgga tccgcatctg
    24361 gaaatcactg aaattgctga ccgcactctg cgtgctggtg ggcctgcgct gttgttcgaa
    24421 aaccctaaag gctactcaat gccggtgctg tgcaacctgt tcggtacgcc aaagcgcgtg
    24481 gcgatgggca tggggcagga agatgtttcg gcgctgcgtg aagttggtaa attattggcg
    24541 tttctgaaag agccggagcc gccaaaaggt ttccgcgacc tgtttgataa actgccgcag
    24601 tttaagcagg tgttaaacat gccgacaaag cgactgcgtg gtgcgccctg ccaacaaaaa
    24661 atcgtctctg gcgatgacgt cgatctcaat cgcattccca ttatgacctg ctggccggaa
    24721 gatgccgcgc cgctgattac ctgggggctg accgtaacgc gcggcccgca taaagagcgg
    24781 cagaatctgg gcatttatcg ccagcagctg attggtaaaa acaaactgat tatgcgctgg
    24841 ctgtcgcatc gcggcggcgc gctggattat caggagtggt gtgcggcgca tccgggcgaa
    24901 cgtttcccgg tttctgtggc gctgggtgcc gatcctgcca cgattctcgg tgcagtcacc
    24961 cccgttccgg atacgctttc agagtatgcg tttgccggat tgctacgcgg caccaaaacc
    25021 gaagtagtaa agtgtatttc caatgacctc gaagtgccag ccagtgccga aatcgtcctg
    25081 gaagggtata tcgatcctgg tgagatggcg ccggaagggc cgtatggtga ccacacaggt
    25141 tactataatg aagtcgataa tttcccggtg tttaccgtga cgcatattac ccagcgtgaa
    25201 gatgcgattt accattccac ctataccggg cgtccgccag atgagcccgc ggtactgggc
    25261 gtggcgttga acgaagtgtt tgtaccgatt ctgcaaaaac agttcccgga aattgtcgat
    25321 ttttacctgc cgccggaagg ctgttcttat cgtctggcgg tagtgacgat caaaaaacag
    25381 tacgccggac acgcgaagcg cgtcatgatg ggcgtctggt cgttcttacg ccagtttatg
    25441 tacactaaat ttgtgatcgt ttgcgatgat gacgtcaacg cacgcgactg gaacgatgtg
    25501 atttgggcga ttaccacccg tatggacccg gcgcgggata ctgttctggt agaaaatacg
    25561 cctattgatt atctggattt tgcctcgcct gtctctgggc tgggttcaaa aatggggctg
    25621 gatgccacga ataaatggcc gggggaaacc cagcgtgaat ggggacgtcc catcaaaaaa
    25681 gatccagatg ttgtcgcgca tattgacgcc atctgggatg aactggctat ttttaacaac
    25741 ggtaaaagcg cctgatgcgc gtttgttttg ccctatttat cgatccgaca gagaaagcgc
    25801 atgacaacct taagctgtaa agtgacctcg gtagcagcta tcacggatac cgtatatcgt
    25861 gtccgcatcg tgccagacgc ggccttttct tttcgtgctg gtcagtattt gatggtagtg
    25921 atggatgagc gcgacaaacg tccgttctca atggcctcga cgccggatga aaaagggttt
    25981 atcgagctgc atattggcgc ttctgaaatc aacctttacg cgaaagcagt catggaccgc
    26041 atcctcaaag atcatcaaat cgtggtcgac cttccccacg gagaagcgtg gctgcgcgat
    26101 gatgaagagc gtccgatgat tttgattgcg ggcggcaccg ggttctctta tgcccgctcg
    26161 attttgctga cagcgttggc gcgtaaccca aaccgtgata tcaccattta ctggggcggg
    26221 cgtgaagagc agcatctgta tgatctctgc gagcttgagg cgctttcgtt aaagcacctt
    26281 ggtctgcaag tggtgccggt ggttgaacaa ccggaagcag gctggcgtgg gcgtactggt
    26341 accgtgttaa cggcggtatt gcaggatcat ggtacgctgg cagagcatga tatctatatt
    26401 gccggacgtt ttgagatggc gaaaattgcc cgtgacctgt tttgcagtga gcgtaatgcg
    26461 cgggaagatc gcctgtttgg cgatgcgttt gcatttatct gagatataaa aaaacccgcc
    26521 cctgacaggc gggaagaacg gcaactaaaa ctttattcag tggcatttag atctatgtcg
    26581 catccggcaa ttggtgcacg atgcctgatg cgacgctggc gcgtcttatc aggcctacat
    26641 tggtgccgga tcggtagacc ggataaggcg ttcacgccgc atccggcaag tggttaaacc
    26701 cgctcaaaca ccgtcgcaat accctgaccc agaccgatac acatcgtcgc cagaccaaac
    26761 tgaacgtctt tgcgttccat cagattcagc agcgtggtgc tgatacgcgc accggaacaa
    26821 cccagcggat gacccagcgc gatcgcacca ccattgaggt tgatcttctc gtcaatctgc
    26881 tccattagcc cgagatcttt aatgcacggc aggatctgcg cggcaaaggc ttcgttcatc
    26941 tcaaacacgc cgatatcgct ggcagaaagc cccgcttttt tcagcgccag cttcgaggcc
    27001 ggaaccgggc cgtaacccat aatcgatggg tcacaaccaa cgaccgccat cgaacgcaca
    27061 cgagcgcgcg gcttaagacc taattcatgg gcgcggcttt cactcatcac cagcatggca
    27121 gctgcgccat cggaaagtgc agaagatgtg cccgccgtta ccgtaccgtt tactggatca
    27181 aacgccggac gcagcgtggc gagggcttcc acggtggttt ccgggcgaat cacttcgtcg
    27241 taattaaact gcttcaggac gccgtcggca tcgtgaccac cagttgggat gatttcattt
    27301 ttaaatgcgg ccgactgcgt ggcggcccag gcgcgggcgt gtgaccgcgc ggcaaaggca
    27361 tcctgcattt cacggctgat accgtgcata cgcgccagca tttctgccgt taagcccatc
    27421 atgcccgccg ctttggcgac attgcggctc aggccggggt gaaaatcgac gccgtgactc
    27481 atcggcacat ggcctatatg ctccacgccg ccaaccagac atgcctgcgt atcgccagtc
    27541 atgatcattc gtgctgcgtc atgcagtgcc tgcatggatg aaccacacaa gcgattgacg
    27601 gtaaccgccg ggacagagtg cggcacttct gccagcagcg ccgcgttacg ggcgatgtta
    27661 aaaccctgct ccagcgtctg ttgcacacaa ccccagtaaa tatcgtcgag ggccgccgct
    27721 tccagcgccg ggttacgcgc cagcaggcta cgcattaaat gagcggagag atcttccgca
    27781 cgcacgttac gaaaagcacc gcccttcgaa cggcccatcg gggtgcgaat tgcatcgaca
    27841 atgacaacct gttccattgt gactccttaa gccgttttca ggtcgccaac cggacgggct
    27901 ggctcaaccg gaggatagta cggttcgtta tgacgcgctt tattacgcag accttccggc
    27961 acttcataca gcgggccgag gtgctgatat tgctgtgcca tatcgaggta ttttgcgcta
    28021 ccgagggtgt ccagccagcg gaacgcgccg ccgtggaacg gagggaagcc caggccgtag
    28081 accagcgcca tatccgcttc cgccggagtg gcgataatgc cctcttccag acagcgcacc
    28141 acttcgttga ccatcgggat catcatgcgg gcgataatct cttcttcgct gaaatcgcgc
    28201 ttcggttggc tcacttctgc cagcaggtct tcaacggcgg cgtcttcttc tttcttcggc
    28261 ttacctttgc tgtcttcttt ataacgccag aaaccgaggc cgttcttctg accaaagcgg
    28321 ttggcatcaa acagcgcgtc gatggcatcg cggtaatctt tctgcatccg ctgcgggaag
    28381 cctgctgcca tgacagcctg agcgtgatgc gcggtatcaa tgcccacaac gtccagcaga
    28441 tatgccgggc ccatcggcca gccaaactgt ttttccatca ctttgtcgat cttgcggaaa
    28501 tccgcgccgt cgcgcagcag ctggctgaaa ccggcgaaat acgggaacag cacgcggtta
    28561 acaaagaagc cggggcagtc gttaaccaca atcggcgtct tgcccatctt gctcgcccag
    28621 gcgacaactt tcgcgatggt ttcgtcggag cttttctcgc cgcgaataat ttctaccaac
    28681 ggcattcggt ggaccgggtt aaagaagtgc atcccgcaga agttttccgg gcgttccagc
    28741 gcgttggcca gttcgctgat aggaatggtt gaagtgttag acgccagcac ggtatcctgg
    28801 cgtacttttt gttcggtttc tgccagtacg gcttttttca ctttcgggtt ttcaacaacc
    28861 gcttctacca caatatccac gcggtcaaat ccggcgtagt cgagcgttgg gtggatcgtg
    28921 gagatcacgc cagccagttt cagaccatcg attttgccgc gctcaagctg cttgttcagc
    28981 agtttcgcgg cttcggtcat gccgagggtt aacgacttgt cgttgatatc tttcatgaca
    29041 accggcacgc ctttccacgc ggactggtaa gcgataccgc cacccatgat gcctgcgccc
    29101 agcacagcag cctgtttcgg ggtttcaacg tctttggtga gtttcttcgc tttgcctttt
    29161 acatattgat cgttaaggaa aatgccgacc agtgcgcgag cttcgttggt atgcgccagc
    29221 gggacaaaac ttttgttttc cagatttaag gcttcttcac gaccaaaacg ggccgcagct
    29281 tcaatggttt ttactgcggt gatgggggcc ggataatgtt tccccgccgt ttgcgcgacc
    29341 attcctttag cgatggtgaa gctcatggtg gcttcaatct tgctcagttt tagcggttcc
    29401 tgcttcggct gacgtttcgc tttccagtcg aggtcgccgt taatggcctg gcgtaaaatc
    29461 gccatcgccc cttcaatcag tttttctgct ttgactacgc catccaccag accgattttc
    29521 agcgcctgat ccgcgccgac atctttaccg gcggcaatga tttccagtgc actgtcagcg
    29581 cccagcatac gtggcatacg tacagaaccg ccaaagcccg gcatgatgcc cagtttggtt
    29641 tccggcagac cgatgcgcag atccggcgtc gccagacgat aatcggtcgc cagcacgcat
    29701 tcgcagccac cacccagcgc atagccgttg acggcagcaa tggtcggcac cggcagatct
    29761 tccaggcgat taaacacgct attggcaaag tgcagccact gacttaactg ttcttcagga
    29821 acgaggaaca gggacaaaaa ttcggtgata tcagcaccga cgataaaggc tgctttgttc
    29881 gaacgcagca gcagcccttt tagatctgat tgctgttcca gcacgccgat ggcctcgccg
    29941 aggctggcga cggtcgcagt gtcgagttta ttaactgaac ctggggcatc aaataccagt
    30001 tcggcaatgc catcttccag ccagtcaagg tacagggtgt cgcctttgta aagcatgtca
    30061 gtctcctgaa tccgcaaggt aatctggtcg taccagatgc atcggagtgt gtgttttgtg
    30121 ttaaaaatat gcaaataaaa gattaaagaa atgccgatct gatcacgctc ggcagaaatc
    30181 acgctctgga tgaacgatgt gctaagatgc ggagacttaa ggtcaaaaaa acagaagggt
    30241 aaaaaatgga atcactggcc tcgctctata aaaatcatat agctacctta caagaacgga
    30301 ctcgcgatgc gctggcgcgc ttcaagctgg atgcattact tattcactcc ggcgagctgt
    30361 tcaacgtttt tctcgacgat catccctatc cgtttaaagt gaacccgcaa ttcaaagcgt
    30421 gggtgccggt aactcaggtg ccaaactgct ggttgctggt ggatggcgtg aacaagccga
    30481 aactgtggtt ctatctgccg gttgattact ggcacaacgt cgaaccgctg ccgacctcct
    30541 tctggactga agatgtggaa gtgatcgcgc tgccgaaagc cgatggcatt ggtagcctgc
    30601 tgcctgctgc gcgcggcaat atcggttata tcggtccggt gccggaacgt gcgctgcaac
    30661 tgggtattga ggccagcaac atcaacccga aaggggtgat cgactacctg cattattacc
    30721 gctccttcaa aaccgagtac gaactggcct gtatgcgtga agcgcagaaa atggcggtca
    30781 atggtcatcg tgcggcagaa gaagcgttcc gttctggcat gagcgagttc gatatcaata
    30841 ttgcctatct gaccgcgacc ggtcatcgtg ataccgacgt accttacagc aacattgtgg
    30901 cgcttaacga acacgctgcg gtgttgcatt acaccaaact ggaccaccag gcaccggaag
    30961 agatgcgcag cttcctgctg gatgccgggg ccgaatataa cggctatgcg gctgacctga
    31021 cccgtacctg gtcggcaaaa agcgacaacg actacgcaca gctggtgaaa gacgtaaatg
    31081 atgaacaact ggcgctgatc gccaccatga aagcaggcgt cagctatgtg gattaccaca
    31141 tccagttcca tcagcgcatc gccaaactgc tgcgtaaaca tcaaatcatc accgatatga
    31201 gtgaagaagc gatggtcgaa aacgatctca ccggaccgtt tatgccgcac ggtattggcc
    31261 atccgctggg cctgcaggtg catgacgtcg ccggttttat gcaggatgat agcggtacac
    31321 acctcgcggc accggcaaaa tatccgtacc tgcgctacac ccgtattctc cagccgggca
    31381 tggtgttaac catcgaaccg ggtatctact tcattgaatc gcttctggca ccgtggcgtg
    31441 aagggcagtt cagcaagcac ttcaactggc agaaaattga agcactgaaa ccgttcggcg
    31501 gcattcgtat cgaagacaac gtggtgatcc acgaaaacaa cgtggaaaac atgacccggg
    31561 atctgaaact ggcgtgatgg aaagctggtt aattcctgcg gcaccggtca cggtcgttga
    31621 agagatcaaa aagagccgct tcataacgat gttggcgcat accgatggcg ttgaggcggc
    31681 gaaagcgttt gttgaatcgg tgcgggcaga acaccccgat gcccgccacc attgcgtggc
    31741 gtgggtggcg ggtgcgccgg atgattctca acagctgggt ttctctgacg acggggagcc
    31801 ggcgggaacg gcaggtaaac cgatgctcgc ccagctaatg ggcagcggcg tcggggaaat
    31861 taccgctgtg gtagtgcgct actacggcgg catattgctt ggcaccggtg ggttagtgaa
    31921 agcgtatggc ggcggcgtga atcaggcgct gcgccagcta acgacccaac gcaagacgcc
    31981 attaaccgaa tatactttgc aatgtgaata tagtcagtta accggcattg aagcgttgct
    32041 ggggcagtgt gacggcaaaa ttatcaacag tgattatcag gcattcgttc tgctgcgggt
    32101 ggcgcttccg gcggcgaaag tggctgaatt ttccgcaaag ctggcggatt ttagccgtgg
    32161 ttcattgcaa ttgttagcga ttgaagaata atccccactt cgttttgcag aactaaggaa
    32221 gcggcagaga tgcattttcg cgccattacc cgaatcgttg gactactggt catcttattt
    32281 tcagggacca tgattatccc tgggctggta gcactcatct accgggatgg agcgggccgc
    32341 gcttttaccc agaccttttt tgtcgccctc gccattggct ctatgctgtg gtggccgaac
    32401 cgcaaagaga agggcgaact gaaatcccgt gaggggtttc tgatagtggt gctgttttgg
    32461 accgtgctgg gtagtgtcgg tgcgctccct tttatcttct cggaaagccc gaacctcacg
    32521 attaccgatg cgttttttga atctttctct ggcctgacca ccaccggggc cactacgctg
    32581 gtggggctgg attcgctccc tcatgccatc ctcttttatc gccagatgct gcaatggttt
    32641 ggcgggatgg ggatcatcgt gttagcggtt gcgatactgc ctatcctcgg cgtgggtggg
    32701 atgcagctct atcgcgcaga aatgcccggc ccgctgaaag ataacaaaat gcgcccgcga
    32761 attgcggaaa cggcgaaaac tctgtggttg atttatgtct tgctgaccgt cgcctgtgcg
    32821 ctggcgttgt ggtttgccgg aatggatgcc tttgatgcca tcggccatag ctttgcgact
    32881 atcgctattg gcggcttctc gacacatgat gccagtatcg gttatttcga cagtccgact
    32941 attaacacta tcattgctat cttcctgctg atctccggct gtaactacgg tctgcacttt
    33001 tcactgttaa gtgggcgtag tctgaaggtt tattggcgcg atccggaatt tcgcatgttt
    33061 atcggcgtac agtttacgct ggtggttatt tgtacactcg tactgtggtt tcataatgtc
    33121 tacagttcgg cgctgatgac aattaaccag gcgtttttcc aggtggtatc gatggcgaca
    33181 accgccgggt ttacgactga cagcattgcc cgctggccgc tctttttgcc ggtactgctt
    33241 ttatgttcag cttttatcgg cggttgcgcc gggtcaacgg gcggtggcct gaaagtgatc
    33301 cgcatcctgc tgctgtttaa gcaggggaac cgtgagctga aacgactggt gcatccgaac
    33361 gccgtctata gcattaagct ggggaatcgc gcactgccgg aacgtatcct cgaagccgtt
    33421 tggggatttt tctccgccta tgcattggtg tttattgtca gtatgctggc gattatcgcc
    33481 acgggcgtgg atgatttttc tgcctttgcc tcagttgttg cgacattgaa taacctgggg
    33541 ccagggcttg gcgtggttgc tgataacttt accagtatga acccggtggc taaatggatc
    33601 ctgattgcca acatgctgtt tggtcgtctc gaggtcttta cattactggt gctctttacc
    33661 ccgactttct ggcgtgaatg atggagtaat acgtgaaaac attaattctt ttctcaacaa
    33721 gggacggaca aacgcgcgag attgcctcct acctggcttc ggaactgaaa gaactgggga
    33781 tccagacgga tgtcgccaat gtgcaccgca ttgaagaacc acaatgggaa aactatgacc
    33841 gtgtggtcat tggtgcttct attcgctatg gtcactacca ctctgcgttc caggaatttg
    33901 tcaaaaaaca tgcgacgcga ctgaattcga tgccgagcgc cttttattcc gtaaacctgg
    33961 tggcgcgcaa accggagaag cgtactccac agaccaacag ctacgcgcgg aagtttctga
    34021 tgaactcgca atggcgtccc gatcgctgcg cggtcattgc cggggcgctg cgttacccac
    34081 gttatcgctg gtacgaccgt tttatgctca agctgattat gaagatgtca ggcggtgaaa
    34141 cggatacgcg caaagaagtt gtctataccg attgggagca ggtggcgaat ttcgcccgag
    34201 aaatcgccca tttaaccgac aaaccgacgc tgaaataagc ataaagaata aaaaatgcgc
    34261 ggtcagaaaa ttattttaaa tttcctcttg tcaggccgga ataactccct ataatgcgcc
    34321 accactgaca cggaacaacg gcaaacacgc agccgggtca gcggggttct cctgagaatt
    34381 ccgacagaga aagcaaaaat aaatgcttga ctttgtagcg ggaaagcgta ttatgaacac
    34441 cctgcgc
//
LOCUS       DAJTRC010000036        34337 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.036, whole genome shotgun sequence.
ACCESSION   DAJTRC010000036 DAJTRC010000000
VERSION     DAJTRC010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 34337)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34337)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34337
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.036"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..397
                     /locus_tag="OO357_001848"
     CDS             <1..397
                     /locus_tag="OO357_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HCR5886814.1"
                     /translation="QEQARGEQCFRDSEWDLQFHIQVALATQNSALAAIVEKMWTQRS
                     HNPYWKKLHEHIDSRTVDNWCDDHDQILKALIRKDPHAAKLAMWQHLENTKIMLFNET
                     SDDFEFNADRYLFAENPVVHLDTATSGSK"
     gene            742..1404
                     /locus_tag="OO357_001849"
     CDS             742..1404
                     /locus_tag="OO357_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5886815.1"
                     /translation="MELLTQLLQALWAQDFETLANPSMIGMLYFVLFVILFLENGLLP
                     AAFLPGDSLLVLVGVLIAKGAMGYPQTILLLTVAASLGCWVSYIQGRWLGNTRTVQNW
                     LSHLPAHYHQRAHHLFHKHGLSALLIGRFIAFVRTLLPTIAGLSGLNNARFQFFNWMS
                     GLLWVLILTTLGYMLGKTPVFLKYEDQLMSCLMLLPVVLLVFGLAGSLVVLWKKKYGN
                     RG"
     gene            1408..1791
                     /gene="mzrA"
                     /locus_tag="OO357_001850"
     CDS             1408..1791
                     /gene="mzrA"
                     /locus_tag="OO357_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EnvZ/OmpR regulon moderator MzrA"
                     /protein_id="HCR5886816.1"
                     /translation="MQIPRMSLRQLAWSGTVLLLVGTLLLAWSAVRQQESTLAIRAVH
                     QGTTMPDGFSIWHHLDAHGIPFKSITPKNDTLLITFDSSDQSAAAKAVLDRTLPHGYI
                     IAQQDNNSQAMQWLTRLRDNSHRFG"
     gene            1938..2292
                     /locus_tag="OO357_001851"
                     /pseudo
     CDS             1938..2292
                     /locus_tag="OO357_001851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417568.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1090 domain-containing protein"
     gene            2330..2635
                     /locus_tag="OO357_001852"
     CDS             2330..2635
                     /locus_tag="OO357_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF883 domain-containing protein"
                     /protein_id="HCR5886817.1"
                     /translation="MSKEHTTEHLRAELKSLSDTLEEVLSSSGEKSKEELSKIRSKAE
                     QALKQSRYRLGETGDAIAKQTRVAAARADEYVRENPWTGVGIGAAIGVVLGVLLSRR"
     gene            2638..3042
                     /locus_tag="OO357_001853"
     CDS             2638..3042
                     /locus_tag="OO357_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HCR5886818.1"
                     /translation="MADTHHAQGPGKSVLGIGQRIVSIMVEMVETRLRLAVVELEEEK
                     ANLFQLLLMLGLTMLFAAFGLMSLMVLIIWAVDPQYRLNAMIATTVVLLLLALIGGIW
                     TLRKSRKSTLLRHTRHELANDRQLLEEESREQ"
     gene            3032..3331
                     /locus_tag="OO357_001854"
     CDS             3032..3331
                     /locus_tag="OO357_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqjK-like family protein"
                     /protein_id="HCR5886819.1"
                     /translation="MSSKVERERRKAQLLSQIQQQRLDISASSREWLEATGAYDRRWN
                     MLLSLRSWALVGSRVMAIWTIRHPNMLVRWARRGFGVWSAWRLVKTTLKQQQLRG"
     gene            3517..3813
                     /locus_tag="OO357_001855"
     CDS             3517..3813
                     /locus_tag="OO357_001855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008503138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HCR5886820.1"
                     /translation="MKKLEDVGVLVAHILMPILFITAGWGKITAYSGTQQYMEAMGVP
                     GFMLLTAFLFHSNFAEGVNSLMFMKNLTISGGFLLLAITGPGAYSIGRLLNKKW"
     gene            3883..4869
                     /gene="yqjG"
                     /locus_tag="OO357_001856"
     CDS             3883..4869
                     /gene="yqjG"
                     /locus_tag="OO357_001856"
                     /EC_number="1.8.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionyl-hydroquinone reductase YqjG"
                     /protein_id="HCR5886821.1"
                     /translation="MGQLIDGVWHDTWYDTKSTGGKFQRSASAFRNWLTADGAPGPTG
                     TGGFIAEKDRYHLYVSLACPWAHRTLIMRKLKGLEPFISVSVVNPLMLENGWTFDDSF
                     PGATGDTLYQHEFLYQLYLHADPHYSGRVTVPVLWDKKNHTIVSNESAEIIRMFNTAF
                     DALGAKAGDYYPPALQTKIDELNGWIYDTVNNGVYKAGFATSQQAYDEAVAKVFESLA
                     RLEQILGQHRYLTGNQLTEADIRLWTTLVRFDPVYVTHFKCDKHRISDYLNLYGFLRD
                     IYQMPGIAETVNFDHIRNHYFRSHKTINPTGIISIGPWQDLDEPHGRDVRFG"
     gene            5162..5530
                     /locus_tag="OO357_001857"
     CDS             5162..5530
                     /locus_tag="OO357_001857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF805 domain-containing protein"
                     /protein_id="HCR5886822.1"
                     /translation="MDWYLKVLKNYVGFRGRARRKEYWMFILVNIIFTFVLGLLDKML
                     GWQRAGGEGILTIIYGILVFLPWWAVQFRRLHDTDRSAWWALLFLIPFIGWLIIIVFN
                     CQAGTPGENRFGPDPKLEQE"
     gene            complement(5737..6633)
                     /gene="yhaJ"
                     /locus_tag="OO357_001858"
     CDS             complement(5737..6633)
                     /gene="yhaJ"
                     /locus_tag="OO357_001858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YhaJ"
                     /protein_id="HCR5886823.1"
                     /translation="MAKERALTLEALRVMDAIDRRGSFAAAADELGRVPSALSYTMQK
                     LEEELDVVLFDRSGHRTKFTNVGRMLLERGRVLLEAADKLTTDAEALARGWETHLTIV
                     TEALVPTPAFFPLIDKLAAKANTQLAIITEVLAGAWERLEQGRADIVIAPDMHFRSSS
                     EINSRKLYTLMNVYVAAPDHPIHQEPEPLSEVTRVKYRGIAVADTARERPVLTVQLLD
                     KQPRLTVSTIEDKRQALLAGLGVATMPYPMVEKDIAEGRLRVVSPESTSEIDIIMAWR
                     RDSMGEAKSWCLREIPKLFAGK"
     gene            6738..7439
                     /locus_tag="OO357_001859"
     CDS             6738..7439
                     /locus_tag="OO357_001859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HCR5886824.1"
                     /translation="MITTRTARQCGQADYGWLQARYTFSFGHYFDPKLLGYASLRVLN
                     QEVLAPGAAFQPRTYPKVDILNVILDGEAEYRDSEGNHVQASAGEALLLSTQPGVSYS
                     EHNLSKDKPLTRMQLWLDTCPQRENPLIQKLALNMDKQQLIASPEGAMGSLQLRQQVW
                     LHHIVLDKGESANFQLHGPRAYLQSIHGKFHALTHHEEKAALTCGDGAFIRDEANITL
                     VADSPLRALLIDLPV"
     gene            7462..7626
                     /locus_tag="OO357_001860"
     CDS             7462..7626
                     /locus_tag="OO357_001860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708913.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886825.1"
                     /translation="MSKKSTKKRQPAKPVVAKEPARTAKNFGYEEMLSELEAIVADAE
                     TRLAEDEATA"
     gene            complement(7838..9148)
                     /locus_tag="OO357_001861"
     CDS             complement(7838..9148)
                     /locus_tag="OO357_001861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708914.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine dehydratase subunit alpha family protein"
                     /protein_id="HCR5886826.1"
                     /translation="MFDSTLNPLWQRYILAVQEEVKPALGCTEPISLALAAAVAAAEL
                     EGPVERVEAWVSPNLMKNGLGVTVPGTGMVGLPIAAALGALGGNANAGLEVLKDATAQ
                     AITDAKALLAAGKVSVKIQEPCNEILFSRAKVWNGEKWACVTIVGGHTNIVHIETHNG
                     VVFTHQACVAEGEQESPLTVLSRTTLAEILKFVNEVPFAAIRFILDSAKLNCALSQEG
                     LSGKWGLHIGATLEKQCERGLLAKDLSSSIVIRTSAASDARMGGATLPAMSNSGSGNQ
                     GITAIMPVVVVAEHFGADDERLARALMLSHLSAIYIHNQLPRLSALCAATTAAMGAAA
                     GMAWLVDGRYETISMAISSMIGDVSGMICDGASNSCAMKVSTSASAAWKAVLMALDDT
                     AVTGNEGIVAHDVEQSIANLCALASHSMQQTDRQIIEIMASKAR"
     gene            complement(9176..10507)
                     /locus_tag="OO357_001862"
     CDS             complement(9176..10507)
                     /locus_tag="OO357_001862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease"
                     /protein_id="HCR5886827.1"
                     /translation="MEIASNKGVIADASTPAGRAGMSESEWREAIKFDSTDTGWVIMS
                     IGMAIGAGIVFLPVQVGLMGLWVFLLSSVIGYPAMYLFQRLFINTLAESPECKDYPSV
                     ISGYLGKNWGILLGALYFVMLVIWMFVYSTAITNDSASYLHTFGVTEGLLSDSPFYGL
                     VLICILVAISSRGEKLLFKISTDMVLTKLLVVAALGVSMVGMWHLYNVGSLPPLGLLV
                     KNAIITLPFTLTSILFIQTLSPMVISYRSREKSIEVARHKALRAMNIAFGILFVTVFF
                     YAVSFTLAMGHDEAVKAYEQNISALAIAAQFISGDGAAWVKVVSVILNIFAVMTAFFG
                     VYLGFREATQGIVMNILRRKMPAEKINENLVQRGIMIFAILLAWSAIVLNAPVLSFTS
                     ICSPIFGMVGCLIPAWLVYKVPALHKYKGMSLYLIIVTGLLLCVSLFLAFS"
     gene            complement(10782..12146)
                     /gene="tdcG"
                     /locus_tag="OO357_001863"
     CDS             complement(10782..12146)
                     /gene="tdcG"
                     /locus_tag="OO357_001863"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5886828.1"
                     /translation="MISAFDIFKIGIGPSSSHTVGPMNAGKSFIDRLESSGLLTATSH
                     IVVDLYGSLSLTGKGHATDVAIIMGLAGNSPQDVVIDEIPAFIELVTRSGRLPVASGA
                     HIVDFPVAKNIIFHPEMLPRHENGMRITAWKGQEALLSKTYYSVGGGFIVEEEHFGLS
                     HDVETSVPYDFHSAGELLKMCDYNGLSISGLMMHNELALRSKAEIDAGFARIWQVMHD
                     GIERGMNTEGVLPGPLNVPCRAVALRRQLVSSDNISNDPMNVIDWINMYALAVSEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFRRPVNERSIARYFLAAGAIGALYKMNASISG
                     AEVGCQGEIGVACSMAAAGLTELLGGSPAQVCNAAEIAMEHNLGLTCDPVAGQVQIPC
                     IERNAINAVKAVNAARMAMRRTSAPRVSLDKVIETMYETGKDMNDKYRETSRGGLAIK
                     VVCG"
     gene            complement(12218..12607)
                     /locus_tag="OO357_001864"
     CDS             complement(12218..12607)
                     /locus_tag="OO357_001864"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312020.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enamine/imine deaminase"
                     /protein_id="HCR5886829.1"
                     /translation="MKKIIETQRAPGAIGPYVQGVDLGSMVFTSGQIPVCPQTGEIPA
                     DVQDQARLSLENVKAIVVAAGLSVGDIIKMTVFITDLNDFATINEVYKQFFDEHQATY
                     PTRSCVQVARLPKDVKLEIEAIAVRSA"
     gene            complement(12621..14915)
                     /gene="tdcE"
                     /locus_tag="OO357_001865"
     CDS             complement(12621..14915)
                     /gene="tdcE"
                     /locus_tag="OO357_001865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-ketobutyrate formate-lyase/pyruvate
                     formate-lyase"
                     /protein_id="HCR5886830.1"
                     /translation="MKVDIDTSDKLYADAWLGFKGTDWKSEINVRDFIQHNYTPYEGD
                     ESFLAEATPATTELWEKVMEGIRIENATHAPVDFDTNIATTITAHDAGYINQPLEKIV
                     GLQTDAPLKRALHPFGGINMIKSSFHAYGREMDSEFEYLFTDLRKTHNQGVFDVYSPD
                     MLRCRKSGVLTGLPDGYGRGRIIGDYRRVALYGISYLVRERELQFADLQSRLEKGEDL
                     EATIRLREELAEHRHALLQIQEMAAKYGFDISRPAQNAQEAVQWLYFAYLAAVKSQNG
                     GAMSLGRTASFLDIYIERDFKAGVLNEQQAQELIDHFIMKIRMVRFLRTPEFDSLFSG
                     DPIWATEVIGGMGLDGRTLVTKNSFRYLHTLHTMGPAPEPNLTILWSEELPIAFKKYA
                     AQVSIVTSSLQYENDDLMRTDFNSDDYAIACCVSPMVIGKQMQFFGARANLAKTLLYA
                     INGGVDEKLKIQVGPKTAPLMDDVLDYDKVMDSLDHFMDWLAVQYISALNIIHYMHDK
                     YSYEASLMALHDRDVYRTMACGIAGLSVATDSLSAIKYARVKPIRDENGLAVDFEIDG
                     EYPQYGNNDERVDSIACDLVERFMKKIKALPTYRNAVPTQSILTITSNVVYGQKTGNT
                     PDGRRAGTPFAPGANPMHGRDRKGAVASLTSVAKLPFTYAKDGISYTFSIVPAALGKE
                     DPVRKTNLVGLLDGYFHHEADVEGGQHLNVNVMNREMLLDAIEHPEKYPNLTIRVSGY
                     AVRFNALTREQQQDVISRTFTQAL"
     gene            complement(14949..16157)
                     /gene="tdcD"
                     /locus_tag="OO357_001866"
     CDS             complement(14949..16157)
                     /gene="tdcD"
                     /locus_tag="OO357_001866"
                     /EC_number="2.7.2.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708919.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="propionate kinase"
                     /protein_id="HCR5886831.1"
                     /translation="MNEFPVVLVINCGSSSIKFSVLDASDCEVLMSGIADGINSENAF
                     LSVNGGEPAPLAHHSYEGALKAIAFELEKRNLNDSVALIGHRIAHGGSIFTESAIITD
                     EVIDNIRRVSPLAPLHNYANLSGIESAQQLFPGVTQVAVFDTSFHQTMAPEAYLYGLP
                     WKYYEELGVRRYGFHGTSHRYVSQRAHSLLNLAEDDSGLVVAHLGNGASICAVRNGQS
                     VDTSMGMTPLEGLMMGTRSGDVDFGAMSWVASQTNQSLGDLERVVNKESGLLGISGLS
                     SDLRVLEKAWHEGHERAQLAIKTFVHRIARHIAGHAASLRRLDGIIFTGGIGENSSLI
                     RRLVMEHLAVLGVEIDTEMNNRSNSFGERIVSSENAHVICVVIPTNEEKMIALDAIHL
                     GKVNAPAEFA"
     gene            complement(16183..17514)
                     /gene="tdcC"
                     /locus_tag="OO357_001867"
     CDS             complement(16183..17514)
                     /gene="tdcC"
                     /locus_tag="OO357_001867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine transporter TdcC"
                     /protein_id="HCR5886832.1"
                     /translation="MSTSDSIVSSQTKQSSWRKSDTTWTLGLFGTAIGAGVLFFPIRA
                     GFGGLIPILLMLVLAYPIAFYCHRALARLCLSGSNPSGNITETVEEHFGKTGGVVITF
                     LYFFAICPLLWIYGVTITNTFMTFWENQLGFAPLNRGFVALFLLLLMAFVIWFGKDLM
                     VKVMSYLVWPFIASLVLISLSLIPYWNSAVIDQVDLGSLSLTGHDGILITVWLGISIM
                     VFSFNFSPIVSSFVVSKREEYEKDFGRDFTERKCSQIISRASMLMVAVVMFFAFSCLF
                     TLSPANMAEAKAQNIPVLSYLANHFASMTGTKTTFAITLEYAASIIALVAIFKSFFGH
                     YLGTLEGLNGLILKFGYKGDKTKVSLGKLNTLSMIFIMGSTWVVAYANPNILDLIEAM
                     GAPIIASLLCLLPMYAIRKAPSLAKYRGRLDNVFVTVIGLLTILNIVYKLF"
     gene            complement(17536..18525)
                     /gene="tdcB"
                     /locus_tag="OO357_001868"
     CDS             complement(17536..18525)
                     /gene="tdcB"
                     /locus_tag="OO357_001868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional threonine ammonia-lyase/L-serine
                     ammonia-lyase TdcB"
                     /protein_id="HCR5886833.1"
                     /translation="MHITYDLPVAIDDIIEAKQRLAGRIYKTGMPRSNYFSERCKGEI
                     FLKFENMQRTGSFKIRGAFNKLSSLTDAEKRKGVVACSAGNHAQGVSLSCAMLGIDGK
                     VVMPKGAPKSKVAATCDYSAEVVLHGDNFNDTIAKVSEIVEMEGRIFIPPYDDPKVIA
                     GQGTIGLEIMEDLYDVDNVIVPIGGGGLIAGIAVAIKSINPTIRVIGVQSENVHGMAA
                     SFHSGEITTHRTTGTLADGCDVSRPGNLTYEIVRELVDDIVLVSEDEIRNSMIALIQR
                     NKVVTEGAGALACAALLSGKLDQYIQNRKTVSIISGGNIDLSRVSQITGFVDA"
     gene            complement(18624..19562)
                     /gene="tdcA"
                     /locus_tag="OO357_001869"
     CDS             complement(18624..19562)
                     /gene="tdcA"
                     /locus_tag="OO357_001869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TdcA"
                     /protein_id="HCR5886834.1"
                     /translation="MSTILLPKTQHLVVFQEVIRSGSIGSAAKELGLTQPAVSKIIND
                     IEDYFGVELVVRKNTGVTLTPAGQLLLSRSESITREMKNMVNEISGMSSEAVVEVSFG
                     FPSLIGFTFMSGMINKFKEVFPKAQVSMYEAQLSSFLPAIRDGRLDFAIGTLSAEMKL
                     QDLHVEPLFESEFVLVASKSRTCTGTTTLESLKNEQWVLPQTNMGYYSELLTTLQRNG
                     ISIENIVKTDSVVTIYNLVLNADFLTVIPCDMTSPFGSNQFITIPVEETLPVAQYAAV
                     WSKNYRIKKAASVLVELAKEYSSYNGCRRRQLIEVG"
     gene            19752..20096
                     /gene="tdcR"
                     /locus_tag="OO357_001870"
     CDS             19752..20096
                     /gene="tdcR"
                     /locus_tag="OO357_001870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TdcR"
                     /protein_id="HCR5886835.1"
                     /translation="MTGITIFYGDNIIRYVVNTKKGLRPYFKQLPDNYQAKFELNLMS
                     KFSNFIINKPFSAINTAARHIFSRYLLENKHLFYQYFKISNTGIDHLEQLINVNFFSS
                     DRTSFCECNRFP"
     gene            20352..20892
                     /locus_tag="OO357_001871"
                     /pseudo
     CDS             20352..20892
                     /locus_tag="OO357_001871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417590.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            20914..21261
                     /locus_tag="OO357_001872"
     CDS             20914..21261
                     /locus_tag="OO357_001872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000484576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886836.1"
                     /translation="MFPVSSIGNDISSDLVRRKMNDLPESPIGNNLEALAPGIEKLKQ
                     TSIQMVTLLNALQPSGKCIITGDFQKELAYLQNVILYNDSSLRLDFLGYNTQIIQRSD
                     NTCELTINEPLKN"
     gene            21430..22080
                     /locus_tag="OO357_001873"
     CDS             21430..22080
                     /locus_tag="OO357_001873"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886837.1"
                     /translation="MANTILNHFKLDSTNFTNANMFQVNFVSSTQNATISWDYLLKIT
                     PVLISISDMYSEEKIKFVESCLNELGDITEEQLKIMRFAIIKSIPRATLTDKLENELT
                     KEIYKSSSKINNCLNRIKLPEMKEFSSKKTYDYIDTIIEDYENIKENAYLVVPQINYT
                     MDLNIEDSSSEELIPSRKTKILRTMALRSGNITHMKHITQRINILPERTIRIITTF"
     gene            complement(22588..22964)
                     /gene="rnpB"
                     /locus_tag="OO357_001874"
     ncRNA           complement(22588..22964)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="OO357_001874"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(22997..24142)
                     /gene="garK"
                     /locus_tag="OO357_001875"
     CDS             complement(22997..24142)
                     /gene="garK"
                     /locus_tag="OO357_001875"
                     /EC_number="2.7.1.165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312029.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate 2-kinase"
                     /protein_id="HCR5886838.1"
                     /translation="MKIVIAPDSYKESLSASEVAQAIEKGFREIFPDAQYVSIPVADG
                     GEGTVEAMIAATQGSERHAWVTGPLGEKVNASWGISGDGKTAFIEMAAASGLELVPAE
                     KRDPLVTTSRGTGELILQALESGATNIIIGIGGSATNDGGAGMVQALGAKLCDANGNE
                     IGFGGGSLNTLNDIDISGLDPRLKDCVIRVACDVTNPLVGDNGASRIFGPQKGASEAM
                     IVELDNNLSHYADVIKKALHVDVKDVPGAGAAGGMGAALMAFLGAELKSGIEIVTTAL
                     NLEEHIHDCTLVITGEGRIDSQSIHGKVPIGVANVAKKYHKPVIGIAGSLTDDVGVVH
                     QHGIDAVFSVLTSIGTLDEAFRGAYDNICRASRNIAATLAIGMRNTG"
     gene            complement(24239..25129)
                     /gene="garR"
                     /locus_tag="OO357_001876"
     CDS             complement(24239..25129)
                     /gene="garR"
                     /locus_tag="OO357_001876"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HCR5886839.1"
                     /translation="MTMKVGFIGLGIMGKPMSKNLLKAGYSLVVADRNPEAIADVIAA
                     GAETASTAKAIAEQCDVIITMLPNSPHVKEVALGENGIIEGAKPGTVLIDMSSIAPLA
                     SREISEALKAKGIDMLDAPVSGGEPKAIDGTLSVMVGGDKAIFDKYYDLMKAMAGSVV
                     HTGAIGAGNVTKLANQVIVALNIAAMSEALTLATKAGVNPDLVYQAIRGGLAGSTVLD
                     AKAPMVMDRNFKPGFRIDLHIKDLANALDTSHGVGAQLPLTAAVMEMMQALRADGLGT
                     ADHSALVCYYEKLAKVEVTR"
     gene            complement(25159..25929)
                     /gene="garL"
                     /locus_tag="OO357_001877"
                     /pseudo
     CDS             complement(25159..25929)
                     /gene="garL"
                     /locus_tag="OO357_001877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312031.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxyglucarate aldolase"
     gene            complement(25945..27279)
                     /gene="garP"
                     /locus_tag="OO357_001878"
     CDS             complement(25945..27279)
                     /gene="garP"
                     /locus_tag="OO357_001878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GarP"
                     /protein_id="HCR5886840.1"
                     /translation="MILDTVDEKRKGVHTRYLILLIIFIVTAVNYADRATLSIAGTEV
                     AKELQLSAVSMGYIFSAFGWAYLLMQIPGGWLLDKFGSKKVYTYSLFFWSLFTFLQGF
                     VDMFPLAWAGISMFFMRFMLGFSEAPSFPANARIVAAWFPTKERGTASAIFNSAQYFS
                     LALFSPLLGWLTFAWGWEHVFTVMGVICFVLTALWIKLIHNPTDHPRMSEEELKFISE
                     NGAVVDMDHKKPGSAAASGPKLHYIKQLLSNRMMLGVFFGQYFINTITWFFLTWFPIY
                     LVQEKGMSILKVGLVASIPALCGFAGGVLGGVFSDYLIKRGLSLTLARKLPIVLGMLL
                     ASTIILCNYTNNTTLVVMLMALAFFGKGFGALGWPVISDTAPKEIVGLCGGVFNVFGN
                     VASIVTPLVIGYLVSELHSFNAALVFVGCSALMAMVCYLFVVGDIKRMELQK"
     gene            27654..29225
                     /gene="garD"
                     /locus_tag="OO357_001879"
     CDS             27654..29225
                     /gene="garD"
                     /locus_tag="OO357_001879"
                     /EC_number="4.2.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate dehydratase"
                     /protein_id="HCR5886841.1"
                     /translation="MANIEIRQETPTAFYIKVHDTDNVAIIVNDNGLKAGTRFPDGLE
                     LIEHIPQGHKVALLDIPANGEIIRYGEVIGYAVRAIPRGSWIDESMVVLPEAPPLHTL
                     PLATKVPEPLPPLEGYTFEGYRNADGSVGTKNLLGITTSVHCVAGVVDYVVKIIERDL
                     LPKYPNVDGVVGLNHLYGCGVAINAPAAVVPIRTIHNISLNPNFGGEVMVIGLGCEKL
                     QPERLLTGTDDVQAIPVESASIVSLQDEKHVGFQSMVEDILQVAERHLQKLNQRQRET
                     CPASELVVGMHCGGSDAFSGVTANPAVGYASDLLVRCGATVMFSEVTEVRDAIHLLTP
                     RAVNEEVGKRLLEEMEWYDNYLNMGKTDRSANPSPGNKKGGLANVVEKALGSIAKSGK
                     SAIVEVLSPGQRPTKRGLIYAATPASDFVCGTQQVASGITVQVFTTGRGTPYGLMAVP
                     VIKMAPRTELANRWFDLMDINAGTIATGEETIEEEGWKLFHFILDVASGKKKTFSDQW
                     GLHNQLAVFNPAPVT"
     gene            complement(29277..30101)
                     /gene="agaR"
                     /locus_tag="OO357_001880"
     CDS             complement(29277..30101)
                     /gene="agaR"
                     /locus_tag="OO357_001880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aga operon transcriptional regulator AgaR"
                     /protein_id="HCR5886842.1"
                     /translation="MSNTDASGEKRVTGTSERREQIIQRLRQQGSVQVNDLSALYGVS
                     TVTIRNDLAFLEKQGIAVRAYGGALICDSTTPSVEPSVEDKSALNTAMKRSVAKAAVE
                     LIQPGHRVILDSGTTTFEIARLMRKYTDVIAMTNGMNVANALLEAEGVELLMTGGHLR
                     RQSQSFYGDQAEQSLQNYHFDMLFLGVDAIDLERGVSTHNEDEARLSRRMCEVAERII
                     VVTDSSKFNRSSLHKIIDTQRIDMIIVDEGIPADSLEGLRKAGVEVILVGDHASSL"
     gene            30350..31630
                     /gene="kbaZ"
                     /locus_tag="OO357_001881"
     CDS             30350..31630
                     /gene="kbaZ"
                     /locus_tag="OO357_001881"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit KbaZ"
                     /protein_id="HCR5886843.1"
                     /translation="MKHLTEMVRQHKAGKTNAIYAVCSAHPLVLEAAIRYASANQTPL
                     LIEATSNQVDQFGGYTGMTPADFRGFVCQLADSLNFPQDALILGGDHLGPNRWQNLPA
                     AQAMANADDLIKSYVAAGFKKIHLDCSMSCQDDPIPLTDDIVAERAARLAKVAEETCR
                     EHFGEADLEYVIGTEVPVPGGAHETLSELAVTTPDAARATLEAHRHAFEKQGLNAIWP
                     RIIALVVQPGVEFDHTNVIDYQPAKASALSQMVENYETLIFEAHSTDYQTPQSLRQLV
                     IDHFAILKVGPALTFALREALFSLAAIEEELVPAKACSGLRQVLENVMLDRPEYWQSH
                     YHGDGNARRLARGYSYSDRVRYYWPDSQIDDAFAHLVRNLADSPIPLPLISQYLPLQY
                     VKVRSGELQPTPRELIINHIQDILAQYHTACEGQ"
     gene            31653..32126
                     /gene="agaV"
                     /locus_tag="OO357_001882"
     CDS             31653..32126
                     /gene="agaV"
                     /locus_tag="OO357_001882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIB"
                     /protein_id="HCR5886844.1"
                     /translation="MPNIVLSRIDERLIHGQVGVQWVGFAGANLVLVANDEVAEDPVQ
                     QNLMEMVLAEGIAVRFWTLQKVIDNIHRAADRQKILLVCKTPADFLTLVKGGVPVNRI
                     NVGNMHYANGKQQIAKTVSVDAGDIAAFNDLKAAGVECFVQGVPTEPAVDLFKLL"
     gene            32137..32916
                     /locus_tag="OO357_001883"
     CDS             32137..32916
                     /locus_tag="OO357_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS
                     mannose/fructose/sorbose/N-acetylgalactosamine transporter
                     subunit IIC"
                     /protein_id="HCR5886845.1"
                     /translation="MEISLLQAFALGIIAFIAGLDMFNGLTHMHRPVVLGPLVGLVLG
                     DLHTGILTGDTLELVWMGLAPLAGAQPPNVIIGTIVGTAFAITTGVKPDVAVGVAVPF
                     AVAVQMGITFLFSVMSGVMSRCDRMAENADTRGIERVNYLALLALGTFYFLCAFLPIY
                     FGAEHAKTIIDVLPQRLIDGLGVAGGIMPAIGFAVLLKIMMKNVYIPYFILGFVAATW
                     LKLPVLAIAAAALAMALIDLLRKSPEPTQPAAQKEEFEDGI"
     gene            32906..33715
                     /locus_tag="OO357_001884"
     CDS             32906..33715
                     /locus_tag="OO357_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HCR5886846.1"
                     /translation="MASNQTTLPNVSENEETLLTGVNENVYEDRSIAAELTKKDINRV
                     AWRSMLLQASFNYERMQASGWLYGLLPALKKIHTNKRDLARAMKGHMGFFNTHPFLVT
                     FVIGIILAMERSKQDVNSIQSTKIAVGAPLGGIGDAMFWLTLLPICGGIGASLALQGS
                     ILGAVVFIVLFNVVHLGLRFGLAHYAYRMGVAAIPLIKANTKKVGHAASIVGMTVIGA
                     LVATYVRLSTTLEITAGDAVVKLQADVIDKLMPAFLPLVYTLTMFWLVRRG"
     gene            33802..34236
                     /locus_tag="OO357_001885"
     CDS             33802..34236
                     /locus_tag="OO357_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HCR5886847.1"
                     /translation="MLSIILTGHGGFASGMEKAMKQILGDQSQFIAIDFPETSSAALL
                     TSQLEEAIAQLDCEDGIVFLTDLLGGTPFRVASTLAMQKPGCEVITGTNLQLLLEMVL
                     EREGLSGEEFRVQALECGHRGLTSLVDELGRCHEECPVEEGI"
BASE COUNT         8895 a         8869 c         8177 g         8396 t
ORIGIN      
        1 tcaggaacag gcgcgcggcg aacaatgctt ccgtgattcc gagtgggatt tacagttcca
       61 cattcaggta gccctggcga cgcagaactc cgccctggcg gccatcgttg aaaaaatgtg
      121 gacccagcgt agccataacc cgtactggaa aaaactacac gaacacattg attcccgtac
      181 cgttgataac tggtgtgatg accacgatca aattctcaag gcgttaattc gcaaagatcc
      241 tcatgctgct aagctggcaa tgtggcagca tctggaaaac accaaaatca tgttatttaa
      301 cgaaaccagc gacgatttcg agttcaatgc cgaccgctat ctgttcgccg aaaacccggt
      361 agtgcatctc gatacggcca ccagcggcag caaatgaaat tcctgtccga caggcgctgc
      421 aatgcgcctg tttgtctcgt cggtaagcga aaagtataaa gtgtcagcct gtgtaaatcc
      481 tctcgccacc ctcccctgca atcagcaaaa tcagactcca cggacatgta attttgataa
      541 cgaacaacgt tgacctttgt tacaattaga ttcaatttga atttatgctt ttgaatgctt
      601 tcttatctca cgttttaaca gggaatagtt caggctgtgt tgatgtatca aacccgcaga
      661 acataccaaa acagcaataa cattgcggta gtgcatcttt taaaaccagc gtggcgttaa
      721 ccgattcacc aggaataatg aatggaactt ttgacccaat tgctgcaagc cctgtgggcg
      781 caggattttg aaaccctggc caatccatcg atgattggca tgttgtattt tgtcttgttt
      841 gtaattttgt tccttgaaaa tggcttgctt ccggcggcct ttttaccggg cgacagttta
      901 ctggtattgg tcggcgtgtt gattgcgaaa ggcgcgatgg gttacccgca aacgattctg
      961 ctgctgaccg ttgccgccag cctcggttgc tgggtcagct atattcaggg gcgatggctg
     1021 ggcaataccc gcaccgtaca aaactggcta tctcatttac ccgcgcatta tcatcaacgc
     1081 gcacaccatc tttttcataa acacggttta tcggcgctgt taattggtcg ctttattgca
     1141 tttgtcagaa cactgctgcc gacgattgcc gggttatcag ggctgaataa cgcgcgcttt
     1201 cagttcttca actggatgag cggtctgctg tgggtattga tcctgacaac tctgggttac
     1261 atgctcggca aaacgccggt atttttaaag tacgaggacc agctgatgtc atgcctgatg
     1321 ctgctcccgg tggtgctgct ggtgtttggc ctggcaggtt ctctggtcgt gttatggaaa
     1381 aagaaatatg gaaatcgggg gtaagggatg caaatacctc gcatgtcgct tcgccagcta
     1441 gcctggtccg gcactgtttt acttctggtc ggcacgctgt tgctggcctg gtccgcggtt
     1501 cgccagcaag agtctacgct ggcgattcgt gccgttcatc aaggcacaac gatgccagac
     1561 ggtttttcga tctggcatca ccttgacgct catggcattc ctttcaaaag tatcaccccc
     1621 aaaaacgaca ctctgttaat tacatttgac tccagcgacc agagcgccgc cgcaaaagcg
     1681 gtcctcgaca gaacattgcc ccatggctac atcattgcgc agcaggacaa taacagtcag
     1741 gctatgcagt ggctgacccg gttacgggat aactctcatc gcttcggata acttcctgtt
     1801 atccgaaaca tatcactcac tttggtgatt tcaccgtaac tgtctatgat taatgaggcg
     1861 gtggttaaca ctaccgcttc gttgtctgga caatcgttcc tttgtaatag gtccgaatca
     1921 caatggaagg ttcaagaatg aaataccgca tcgctttagc tgtttctctc tttgctctta
     1981 gtgccggtag ttatgccact accctgtgtc aggaaaagga gcaaaatatc cttaaggaga
     2041 tcagctatgc cgaaaaacac caaaaccaga atcgtattga cggtctgaat aaagccctga
     2101 gtgaagtccg ggccaactgt tcagatagcc agctgcgtgc cgatcatcac aagaaaatcg
     2161 caaagcagaa agatgaggtg gcggaacgcc agcaagattt agccgaggcg aagcaaaaag
     2221 gcgatgccga tagaacggaa actggcagaa gcgcaggaag agctgaaaaa gctggaagcg
     2281 cgcgactact aactcacaat agtcactact tactcacctg gagaaaacta tgtcgaaaga
     2341 acacactacg gaacatctgc gtgctgagtt gaaatccctt tccgatacgc tggaagaggt
     2401 gcttagctca tctggcgaga agtcgaaaga agagttgagt aagattcgta gcaaagcgga
     2461 gcaggcactg aaacagagcc gttatcgcct gggtgaaacc ggtgatgcca tcgccaaaca
     2521 aacccgtgtc gcggcggcgc gtgctgatga gtatgtgcgt gaaaatccgt ggaccggcgt
     2581 gggaattggc gctgcaatcg gtgtagtgct cggcgttctg ctgtcgcgtc gttaattatg
     2641 gcggacactc atcacgcgca agggcccggt aaaagcgttc tgggcatcgg gcagcgaatt
     2701 gtttccatca tggttgaaat ggtggaaaca cgtctgcggc tggcggtggt ggagctggaa
     2761 gaggaaaaag ctaatctctt tcaactttta ctgatgctgg gcctgacgat gcttttcgct
     2821 gcatttggtc ttatgagcct gatggtgcta attatttggg cggttgatcc gcaatatcgc
     2881 ctgaatgcga tgattgccac caccgtggtg ttgctgctac tggcactgat tggcggtatc
     2941 tggacgttac gtaaatcgcg taaatccacc ttgctgcgcc atacacgcca tgagctggca
     3001 aacgatcggc aactgctcga ggaggagtcc cgtgagcagt aaagtcgaac gtgaacgacg
     3061 taaggcgcaa ctgcttagcc agatccagca acaacggctg gatatttccg ccagtagtcg
     3121 tgaatggctg gaggcaacag gcgcttacga tcgtcgctgg aatatgctgc taagtctgcg
     3181 ctcctgggcg ctggttggca gtagagtgat ggcgatctgg acgattcgcc atcctaatat
     3241 gctggtccgc tgggccagac gcggttttgg cgtatggagc gcctggcgtc tggttaaaac
     3301 gaccctcaag cagcaacagc ttcgcggtta acttccctct ggccggagca attccggcct
     3361 tatccatcaa attttttgaa tatttttgac agttttcctt gctaacaatc atcattcacc
     3421 acgtttatga ttctctccat cgacagcaac gacgctaata gcgcgccctt gcacaaaaaa
     3481 acaatcagca gcctgagtgg cgcagtggag agtatgatga aaaaattaga agatgttggt
     3541 gtactggtag cgcacatttt aatgccgatt ctgtttatta ccgcaggctg gggaaaaatc
     3601 accgcttact ctggtaccca acaatatatg gaagcaatgg gtgtcccggg ttttatgctg
     3661 ctgacggcat ttttatttca cagcaacttt gctgaaggcg tcaactcgct gatgttcatg
     3721 aaaaacctga caatttctgg cggattcctg ctgctggcaa ttaccggtcc gggcgcgtat
     3781 agcatcggcc gcctgctgaa taaaaagtgg taagcacgcg ctatacttaa cgataaaaaa
     3841 cgaggaggaa gctcctcgtt tttgctattg gaggagagaa aaatgggtca actgattgac
     3901 ggcgtctggc atgacacctg gtacgatacc aaatctaccg gcggtaaatt tcaacgttca
     3961 gcttccgcat ttcgtaactg gctcactgcc gatggcgcac ctggccccac tggcacaggc
     4021 ggttttatcg cagagaaaga tcgttatcat ctctatgttt cactcgcctg cccgtgggcg
     4081 caccgcacgc tgatcatgcg caaactcaaa ggactggaac cgtttatttc cgtttccgta
     4141 gtgaacccgc tgatgctgga aaacggctgg acctttgatg acagttttcc gggagcaacc
     4201 ggagatacgc tctatcaaca tgaatttctg tatcagcttt atctccacgc cgatccacac
     4261 tacagcggac gagttactgt tcccgtgctg tgggacaaaa agaaccacac catcgtcagc
     4321 aacgaatcag cagaaatcat ccgcatgttc aataccgcgt ttgatgcgct gggcgcaaaa
     4381 gcgggtgatt actacccacc agccctgcaa acgaaaattg acgaacttaa cggctggatt
     4441 tatgacaccg ttaacaacgg cgtgtataaa gctggttttg ccaccagcca gcaagcctac
     4501 gacgaggcgg tggcgaaagt gtttgaatcg ctggcgcgac tggagcagat tttaggccag
     4561 catcgttacc tgaccggcaa ccagctaacc gaagccgata ttcgcctgtg gactacgctg
     4621 gtgcgttttg atccagtgta tgtgacccac ttcaagtgtg ataagcaccg catcagcgat
     4681 tacctgaatc tgtatggctt cctgcgcgat atctaccaga tgccggggat cgccgaaact
     4741 gtcaatttcg atcacatccg taatcattac ttccgcagcc ataagaccat caaccctacg
     4801 gggattattt caattggtcc gtggcaggat ctcgatgaac cgcatggacg agatgttcgc
     4861 ttcggttaaa aaaaggcacc ttacgaggtg cctctgataa ttagctaagt gcatgttgtt
     4921 catgtcggat gcggcgtgaa cgccttatca aacctacaaa gcgtttatat tcaatatgtt
     4981 acaatgaccg cgtaggccag ataagcgtag cgcatctggc gattttgcgc ttgccatcag
     5041 tcttagcgcg ccttcacggc actttattaa ttccttgaac gaatatttac tgccatttca
     5101 ttcacgtcta ttcttaattt gctgctttaa aaacaagtga ttgagcaaaa ttgaggcaaa
     5161 aatggactgg tatctgaaag tactaaaaaa ttatgtcggt ttccggggtc gtgcgcggcg
     5221 caaagagtac tggatgttta ttctggtcaa catcatcttt acgttcgtgc tggggctgct
     5281 ggataaaatg ttaggctggc aacgcgctgg tggcgaaggc atcctgacga taatctacgg
     5341 tattctggtg tttttaccgt ggtgggcggt tcagttccgc cgcctgcacg acaccgaccg
     5401 ctcggcgtgg tgggcactgc tattcttaat cccgtttatc ggctggctta ttatcatcgt
     5461 ctttaactgc caggcgggta cgccaggcga aaaccgcttt ggaccagatc caaaactgga
     5521 acaagaataa tgattaagca ccttgtaaaa ggtgcttcaa attgttgaca aagtgcgctt
     5581 tgtttatgcc ggatgcggcg taaacgcctt atccagccta caaattgcca aaattcaata
     5641 tattgcagtg actgcgtagg cctgataagc gtagcgcatc aggcagtttt gcgtttgtca
     5701 gcagtcagaa gcaccttgtg aaaggtgctt aatagattat tttccagcga acaactttgg
     5761 aatttcccgc agacaccagg atttcgcttc ccccatactg tcacgacgcc aggccataat
     5821 aatatcgatc tcgctggtcg attccgggct gacgacacgc aaccgccctt ccgcaatatc
     5881 tttttcgacc atcggatacg gcatcgtcgc cacgccaagc cccgccagta atgcctgacg
     5941 tttatcttca atcgtgctca ccgttaagcg cggctgtttg tccagcagct gtacggtcaa
     6001 caccgggcgc tcacgagcgg tatccgctac cgcaattcca cgatatttca cacgcgtcac
     6061 ttcagataac ggttccggct cctgatgaat cgggtgatca ggcgcggcaa cgtagacgtt
     6121 cattaacgta tagagcttgc gcgagttgat ctccgacgag gaacgaaaat gcatatccgg
     6181 cgcgataacg atatccgccc gcccctgctc cagccgttcc cacgcccccg ccagcacttc
     6241 tgtgatgatt gccagttggg tattggcttt tgccgccagt ttgtcgatta acgggaaaaa
     6301 ggcaggtgtc ggtaccagcg cttcggtcac aatggtgaga tgcgtttccc aaccgcgcgc
     6361 gagagcttcc gcatcagtag tcagtttatc tgcggcttcc agcaaaacgc gccctcgctc
     6421 cagcaacatc ctcccgacat tggtgaattt ggtgcgatgg cccgagcggt caaacagcac
     6481 cacatccagc tcttcttcca gtttttgcat ggtgtagcta agtgcggaag gcacgcgtcc
     6541 cagctcatcc gctgccgccg caaaactgcc ccggcgatcg atcgcatcca taacccgtag
     6601 tgcttccagc gttaatgccc tttctttggc catttcgttc tcattcagga aatttgaaca
     6661 taccgggcag aatatctggc taacaatgta gcgtccagcc ccttaccata aaaggaagta
     6721 aagagaggtc aagaattatg attactaccc gaactgccag gcagtgtgga caagcagact
     6781 acggatggtt gcaggcccgg tatacttttt cctttggaca ctacttcgac ccgaaattgt
     6841 taggctatgc ctccctgcgt gtgcttaacc aggaagtgct ggccccaggt gccgcctttc
     6901 agccgcgaac ctatcccaaa gtcgatattt taaatgtgat tctggatgga gaagcagagt
     6961 atcgcgacag cgaaggcaat catgttcagg ccagcgccgg agaggcgttg ctgctctcta
     7021 cccagccggg tgtcagctat agcgaacaca atctcagcaa agacaaaccg ttaacgcgaa
     7081 tgcagctttg gctggatacc tgcccgcagc gagagaatcc gctgattcaa aagctggcgc
     7141 ttaatatgga caagcagcaa ttaatcgcct cgccagaggg tgcgatggga agcctgcaat
     7201 tacgccaaca agtgtggctg caccatatcg tgctcgacaa aggcgaaagt gcgaatttcc
     7261 agttgcatgg gccacgcgcg tatttgcaat cgattcacgg gaaatttcat gcgcttacgc
     7321 atcatgaaga gaaagcggcg ctaacctgcg gtgatggggc gtttattcgt gacgaggcta
     7381 acattacgct ggttgccgat tccccactgc gcgctttgct gatagatttg cctgtctagt
     7441 tgtttttaca gggagatgat gatgagtaaa aaatcgacca aaaagcgcca gccagcgaag
     7501 cccgtggtgg cgaaagaacc tgctcgcacc gccaaaaatt ttggctatga agagatgttg
     7561 agcgagctgg aagctatcgt cgcggatgct gaaacgcgtt tagccgagga tgaagctacc
     7621 gcgtaaagac aggttgccgg atgcggcgta aacgccttat ccggcctaca aaacacaaca
     7681 acatcaacaa attaaacacc ccgtaggccg gataagatgc gccagcatcg catccggcat
     7741 caggtacaaa acacaacaac atcaacaagt tgagcacccc gtaggccgga taaggtgcgc
     7801 cagcatcgca tccggcacaa tactcaaaac ctggcgttta tctggccttg ctcgccataa
     7861 tctcgataat ctgccgatcc gtttgctgca tcgaatggct tgctaacgca cacaggttgg
     7921 caatcgactg ctcaacatca tgcgccacga tcccttcatt gccggtcacg gcggtatcat
     7981 ccagcgccat taacaccgct ttccacgcag ccgaagcact ggtcgaaacc ttcatcgcgc
     8041 agctgttcga cgcaccatcg caaatcatgc cgctgacatc gccgatcata ctgctgatcg
     8101 ccatcgagat agtttcataa cgcccatcca ccagccatgc catcccggcg gcggccccca
     8161 ttgctgcggt cgttgcggca caaagtgcag acaaacgcgg taactggtta tggatgtaaa
     8221 ttgcgctcaa atgagaaagc atcagcgcac gcgccagtcg ttcatcatcc gctccgaagt
     8281 gttctgctac aaccaccaca ggcattattg cagtgatccc ctggttaccc gagccggagt
     8341 tactcatagc cggaagcgta gcgccgccca tacgcgcatc ggatgccgcg ctggtacgaa
     8401 tcacaatgga tgaagagaga tctttcgcca gcaaaccgcg ctcgcactgt ttttccagcg
     8461 tcgcgccaat atgcagcccc cacttaccgc tcaaaccttc ctgcgataac gcacaattta
     8521 gcttcgcgga atcgagaata aagcggatcg ccgcaaatgg gacttcattg acgaacttca
     8581 ggatctcagc cagcgtcgtt ctggaaagca ccgtcagcgg cgactcttgc tcgccctctg
     8641 ccacacacgc ctggtgggta aacaccacac cattgtgcgt ctcaatatgc acaatgttgg
     8701 tatgcccgcc gacgatggtg acacacgccc acttctcacc gttccagact ttggcgcgtg
     8761 agaagaggat ttcattgcaa ggttcctgga tcttaacaga gactttcccc gccgccagca
     8821 gtgctttggc atcggtaatt gcctgcgcag ttgcgtcttt cagcacttcc agcccggcgt
     8881 tggcatttcc acctaacgcc cccagcgccg ccgcaatcgg cagccccacc attcccgtgc
     8941 cgggaacggt gacgcccaga ccgttcttca tcagatttgg cgaaacccag gcttctacac
     9001 gttcaaccgg accttccagt tctgccgcag caaccgccgc cgccagcgcc agtgaaatcg
     9061 gttcagtaca tcccagcgcc ggttttactt cctcctgaac ggcgaggatg taacgctgcc
     9121 ataacggatt taaagtcgaa tcaaacatat taaaaacctt aaaatttcag gtaaatcaag
     9181 aaaatgccag gaacagagaa acacaaagca acaaaccagt gacgataatc aggtacagag
     9241 acatcccttt gtatttgtgc aatgccggta ctttgtaaac cagccacgcc gggatcaggc
     9301 accctaccat gccgaaaatc gggctacaga tagaggtgaa gctcaacacc ggtgcgttca
     9361 gtacgatggc gctccaggcc agcaaaatgg cgaaaatcat gatgccgcgc tgaacaagat
     9421 tttcgttaat cttctcggca ggcatcttgc gacgcaggat gttcattacg atcccttgcg
     9481 ttgcttcgcg aaagcctaaa tagacgccaa agaatgcagt catgacagca aagatattga
     9541 gaatgacgct gaccactttc acccacgctg cgccgtcacc gctaataaac tgtgcggcaa
     9601 tcgccagcgc ggaaatattc tgctcgtagg cttttaccgc ttcgtcatgt cccatcgcca
     9661 gcgtgaacga cacggcgtag aaaaagacgg tgacaaacaa aatgccaaac gcgatattca
     9721 ttgcccgcaa tgctttatgc cgcgccactt caattgattt ttcccgcgag cgataagaga
     9781 tcaccatcgg gcttaacgtc tggataaaca gaatcgacgt cagggtaaac ggcagcgtaa
     9841 taatggcgtt tttcaccagc agccccagcg gcggtagcga accgacgtta tacagatgcc
     9901 acattcccac catcgacacg cccagcgcag cgactaccag cagcttggtc agcaccatgt
     9961 cggtcgaaat tttgaataac aatttctcgc cgcgtgaaga gatcgccacc agaatgcaaa
    10021 tcagtaccag accataaaag ggactgtctg acagcaaccc ttccgtcacg ccgaaggtat
    10081 gcagatagga agcactatcg ttggtgatgg cggtggaata gacgaacatc caaatcacca
    10141 gcattacgaa atagagcgca cctaacagga tgccccagtt tttacctaaa taaccgctaa
    10201 tgacactcgg gtaatcttta cattctggtg attctgccag cgtattaata aacaaccgct
    10261 gaaacagata cattgccggg taaccaatca ccgatgagag caaaaatacc cacaatccca
    10321 tcaaaccgac ctgcaccggg agaaaaacaa tccccgcgcc aatcgccatc ccgatactca
    10381 taatcaccca gccggtgtca gtactgtcga atttgatcgc ttctcgccac tcgctctcac
    10441 tcattccagc acgacccgcc ggggtcgaag cgtctgcaat gacgccttta ttcgatgcaa
    10501 tttccataat ttctcgctca atattttgta gggcttatta tttttttccg agccgcatca
    10561 aggcgatatg cggtctacgt ttttgcaggc gagttattag aataaagaaa tgatacgcgc
    10621 gaggtcagag aaaatcttca caatcaagac tttaaaataa cggagatgga taagaatttt
    10681 ctactaaatt aatcacaaga gaatattcat tctctatttg cggcagatca caaaaaaaag
    10741 gtgcacattt gtgcacccaa ggatgaaagc tgacagcaat gtcagccgca gaccacttta
    10801 atggccagtc ctccgcgtga tgtttcgcgg tatttatcgt tcatatcttt gccggtttca
    10861 tacatcgtct cgatcacttt atcgagtgaa acacgcggtg ccgaggtgcg gcgcatcgcc
    10921 atccgcgcgg cgtttactgc tttcacggca ttaatggcat tacgttcaat gcacgggatt
    10981 tgtacctgtc cggcaaccgg atcgcaggtc agcccaaggt tatgctccat cgcgatttcc
    11041 gccgcattgc atacctgcgc cggactgccg cccagtagtt cagttaaccc tgccgctgcc
    11101 attgaacagg ccacgccaat ctccccctga cagccgacct ccgcgccaga gatggaggcg
    11161 ttcattttat acagcgcgcc aatagccccc gctgccagaa aatagcgggc aattgaccgc
    11221 tcgtttaccg gacgacggaa cttatcgtaa taagccagca ctgccggaat aatgccgcac
    11281 gcaccgttag tcggtgccgt taccacgcgc ccgccagctg cgttttcttc actaaccgcc
    11341 agcgcgtaca tgttgatcca gtcgatgaca ttcatcggat cgttagagat gttatcgcta
    11401 gaaaccagct gacgacgcag cgctacggca cgacacggca cattgagcgg accaggcagc
    11461 acgccttcag tgttcatccc gcgttcaata ccgtcatgca tcacttgcca gatacgggca
    11521 aaccctgcgt caatttccgc tttgctgcgc agcgccagct cgttgtgcat catcaggcca
    11581 gatatagaca ggccgttgta atcacacatt ttcagcagtt cacctgctga gtggaaatcg
    11641 taaggtacgg acgtttcgac atcgtgcgac aggccgaagt gttcttcttc gacaataaac
    11701 ccgccgccga cagagtaata ggttttactt aatagcgctt cctgtccctt ccaggcagtg
    11761 atccgcattc cgttctcatg gcgaggcaac atttcgggat ggaagataat gttctttgct
    11821 acaggaaaat caacaatatg cgcaccagat gccactggca gccgcccgct gcgcgttact
    11881 aactctataa atgcagggat ctcatcaatg acaacatcct gcggactgtt tcctgccagt
    11941 cccatgataa tggcgacatc cgtggcatgg cctttgcccg tcagtgacaa cgacccgtac
    12001 agatcgacca caatatggct cgtcgcggtt aataagccgc tactttccag ccgatcaata
    12061 aaactttttc cggcattcat tggccccacg gtatgcgaac tggagggacc aatcccaatt
    12121 ttgaaaatat cgaatgcact aatcatatcc acaccctcgg attgccgttc agtgaagtgg
    12181 agcggaacga ccttgcgacc gtcccgctca cgaggcttta cgcactacgt actgcgatag
    12241 cttcaatttc cagcttcaca tctttcggca aacgcgcaac ctgcacacag ctccgggtcg
    12301 ggtaggtcgc ctgatgctca tcgaagaact gcttatagac ttcattgatg gtggcaaaat
    12361 cattcagatc ggtgatgaac acggtcatct tgatgatatc gcccacgctc agcccggcag
    12421 caaccacgat cgctttgacg ttttcgaggc ttaaacgcgc ctgatcttgc acatcagccg
    12481 ggatctcacc ggtctgcggg caaaccggaa tttgcccgga ggtgaagacc atgctgccta
    12541 aatcaacgcc ctgaacataa gggccgattg cgcctggggc acgttgcgtt tcgataatct
    12601 ttttcataca tcctccggcg tcagagcgcc tgggtaaaag tacgtgaaat aacatcctgt
    12661 tgctgttcac gggtcagtgc gttgaagcgc acggcgtagc cagagacacg gattgtcagg
    12721 ttaggatatt tttccgggtg ctcgatggca tccagcagca tttcccgatt cattacgttg
    12781 acgttgaggt gttgaccgcc ttcgacatcc gcttcgtggt ggaaataccc atccagcagg
    12841 ccgacaaggt tggttttacg tactggatct tctttgccca gcgccgcagg aacgattgag
    12901 aaggtgtacg agatcccatc tttggcgtag gtgaacggca gtttcgccac cgacgtcaat
    12961 gaggccacgg cacctttgcg gtcacgacca tgcatcgggt tagcgcccgg cgcgaacggt
    13021 gttccggcgc gacgaccgtc cggcgtatta ccggttttct ggccgtacac cacgttagaa
    13081 gtgatagtca gaatcgactg ggtagggacg gcgttgcgat aggttggcag cgctttaatt
    13141 ttcttcataa agcgttcaac caggtcgcag gcaatgctgt ctacgcgctc gtcgttgttg
    13201 ccgtactgcg gatattcacc gtcgatttca aagtccaccg ccaggccgtt ttcgtcacgg
    13261 attggtttca cgcgggcata tttgatggca gacagggagt ccgtcgccac cgacaggccc
    13321 gcgatgccgc atgccatagt gcgatagaca tcacgatcgt gcagcgccat cagcgaagct
    13381 tcgtagctgt acttgtcgtg catgtagtga atgatattca gcgcgctgat gtactgcacc
    13441 gccagccagt ccatgaagtg atcgaggctg tccatcactt tgtcgtaatc cagcacgtcg
    13501 tccatcagcg gtgctgtttt cggcccgacc tgaatcttca gcttctcgtc caccccgccg
    13561 ttaattgcgt agagcagcgt tttcgccagg ttagcgcgtg caccaaagaa ctgcatttgc
    13621 ttaccaatca ccattgggct gacgcagcag gcaatcgcgt aatcgtcgct gttgaagtca
    13681 gtacgcatca gatcgtcatt ttcatactgc aaggaagagg tgacgatcga cacctgcgcg
    13741 gcatattttt tgaaggcaat cggtaattct tccgaccaaa gaatggtcag gttaggttcc
    13801 ggtgccggcc ccatagtgtg cagggtgtgc aaatagcgga aggagttttt ggtcaccagc
    13861 gtacgaccgt ccagccccat cccgccgatc acttccgtcg cccagattgg gtcgccggag
    13921 aacagcgaat caaattccgg tgtacgcagg aagcgtacca tacggatctt catgatgaag
    13981 tgatcgatca gttcctgtgc ctgctgctca ttgaggacgc cagctttaaa gtcgcgctca
    14041 atgtagatat ccaggaacga tgccgtgcgc cccagcgaca tcgcgccgcc attttgcgat
    14101 ttcactgccg ccagataagc gaagtagagc cactgcaccg cttcctgtgc attctgcgcc
    14161 gggcgagaga tatcaaagcc atatttcgcc gccatttcct gaatctgcaa cagcgcatga
    14221 cgatgctctg ccagctcctc acgcagacgg atggtggctt ccagatcctc gcctttttcc
    14281 agacgagact ggagatcggc aaattgcagt tcgcgttcac gcaccagata actgatgcca
    14341 tacagcgcta cgcggcgata gtcaccgata atgcgcccac gaccatagcc atctggtaaa
    14401 ccggtcagca cgccagattt acggcagcgc agcatatccg gtgagtaaac atcaaatacg
    14461 ccctggttat gggttttacg cagatcggta aacagatatt caaattcact gtccatttca
    14521 cggccatagg cgtggaatga acttttaatc atattaatgc caccgaacgg gtgtagcgca
    14581 cgtttcaacg gcgcatccgt ttgcaggcca acaatttttt ccagcggctg gttaatatat
    14641 cccgcatcat gagcggtaat tgtggtggca atattggtat cgaaatcaac cggcgcgtgg
    14701 gttgcgtttt cgatacggat gccttccatt accttttccc acaattccgt ggtggcaggc
    14761 gtcgcttcgg cgagaaaaga ttcatcgcct tcatacggtg tatagttatg ttgaataaaa
    14821 tcgcggacat taatttcgct tttccagtcc gtacctttaa agccaagcca tgcgtcagcg
    14881 tacagcttat cgctggtatc aatatctacc ttcatgaaaa ataatctctc tacaatactt
    14941 caactaaatt atgcaaattc tgcgggcgcg ttaactttac ctaaatgaat ggcatccaaa
    15001 gcaatcattt tttcttcgtt agtcggaata acgacgcaaa tgacatgcgc attttcactg
    15061 gaaacaattc gctcgccaaa agagttagaa cgattattca tttctgtatc aatctctacg
    15121 cctaatacag ccaaatgttc catgaccaga cgacgaatta aacttgaatt ctctcctatt
    15181 ccgccggtga atataattcc atccagccga cgtaatgaag ctgcatgtcc ggcaatatga
    15241 cgggcaattc ggtgaacaaa ggttttaatt gccagttgcg cgcgttcgtg accttcatgc
    15301 caggcttttt ccagaacacg taaatccgaa gaaagaccgg aaatacctaa taatcccgac
    15361 tctttattca ctacgcgttc caggtcaccc aggctctggt tggtttggct ggcgacccag
    15421 gacatcgcac caaagtcgac atcgccactg cgggtaccca tcatcaagcc ttccagcggc
    15481 gtcattccca ttgaggtgtc aacactctga ccgttgcgaa ctgcgcagat tgacgcgcca
    15541 ttgccaagat gcgccacaac caggccggag tcatcttccg ccagattcag cagcgaatgt
    15601 gcgcgctggg aaacatagcg gtgcgacgtg ccgtggaaac cataacggcg tacacctaac
    15661 tcttcataat atttccacgg caggccgtat aaataagctt ccggagccat cgtctggtgg
    15721 aaactggtat caaataccgc cacctgagtt acgcccggaa ataattgctg cgccgattca
    15781 ataccactta aatttgcgta attatgcagg ggtgccagtg gagaaacgcg acggatatta
    15841 tcaatgactt catcggtaat aatggcggac tcggtaaaaa tactgccgcc gtgagcgatg
    15901 cggtggccaa ttaaggccac gctgtcattt aaattccgtt tttccagttc aaatgcaatt
    15961 gccttcaatg caccttcgta gctgtggtga gccagcggtg ctggctctcc cccatttacg
    16021 gataagaatg cattttccga gttaataccg tcggcaatac ctgacattaa tacttcacaa
    16081 tcgctggcat cgagtacgga aaacttaatc gaagacgaac cacagttaat aaccaaaaca
    16141 accggaaatt cattcatctc ttttctcatc ctgagttacg gattaaaaca gtttgtatac
    16201 gatgttcagg atggtcagca gaccaatcac ggtaacaaac acgttatcca gacgaccacg
    16261 gtatttcgcc agagacggcg ctttacggat agcatacatc ggcaacaggc acagcaggga
    16321 tgcgataatc ggtgcgccca tggcttcaat caggtcgagg atgttcgggt tggcgtaggc
    16381 aacaacccag gtggagccca tgatgaagat catgctgaga gtattcagtt tacccagcga
    16441 cactttggtt ttgtcacctt tataaccgaa cttcagaatc agaccattca agccttccag
    16501 cgtccccaga tagtgaccga agaaagattt gaagatagcc acgagtgcga tgatggaagc
    16561 cgcatattcc agtgtaatcg cgaacgttgt tttcgtaccg gtcatggacg caaagtggtt
    16621 agccagataa gaaagcactg gaatattctg cgctttggct tccgccatgt tggccggaga
    16681 cagagtaaac aggcagctaa aggcaaagaa catcaccact gcaaccatca gcatgctggc
    16741 acgagaaatg atttgggaac atttacgttc ggtgaagtcg cgaccgaagt ctttctcata
    16801 ctcttcacgt ttagaaacca cgaaggaaga gacgattggc gagaagttaa aggagaaaac
    16861 catgatggaa atccccagcc agacagtgat caggataccg tcatgaccgg ttaacgacag
    16921 cgaaccgagg tcaacctggt cgataactgc agagttccag taagggatca gcgacagaga
    16981 aatcagcacc aggctggcga taaacggcca taccaggtag ctcatcactt taaccatcag
    17041 atccttacca aaccagatga cgaaagccat cagcagcaac aggaacagcg ccacaaagcc
    17101 gcgattcagc ggtgcaaatc cgagctggtt ttcccagaac gtcataaagg tattggtaat
    17161 agtaacgcca taaatccaca gcagtgggca aatcgcgaag aagtacagga acgtgataac
    17221 cacgccgcca gttttaccaa aatgctcttc caccgtttcc gtaatgttgc cggaagggtt
    17281 agagccagaa agacacagac gcgccagcgc ccggtggcaa taaaatgcga tggggtatgc
    17341 caataccaac atcagaagaa tcgggatcag tccgccaaaa cctgcgcgga tagggaagaa
    17401 cagcaccccg gcgccgattg ccgtaccaaa caagcctaac gtccatgtgg tatctgattt
    17461 acgccaggac gattgttttg tctggctgga tacaatgcta tctgaagtac tcatatccta
    17521 tcctcaacga attaattaag cgtcaacgaa accggtgatt tgagagacgc gagaaagatc
    17581 gatattgccg ccggaaataa tactgacggt ttttctattt tgaatatatt ggtctaattt
    17641 accgcttaat aatgcagcac atgccagagc gcctgcgcct tcggtgacga ctttattgcg
    17701 ctgaattaag gcaatcatac tgtttctgat ttcgtcttcg ctgaccagca cgatgtcatc
    17761 gactaattca cgaacgattt cgtaagttaa attacccggg cgggagacat cacaaccatc
    17821 cgccagggtg ccggtagttc ggtgcgtggt tatttctccg gagtggaaag aagccgccat
    17881 gccgtgaacg ttttctgact gtacgccaat aacacgaatg gtcgggttaa tagatttaat
    17941 tgccaccgca ataccagcaa ttaaaccacc accaccaatt ggcacaatca cgttatcgac
    18001 atcatagaga tcttccataa tttccagacc aatcgttccc tggccagcaa tcactttcgg
    18061 atcatcgtaa ggtgggataa aaatacgacc ttccatttcg acaatttcgc tcactttagc
    18121 gatagtgtcg ttgaagttat caccatgcag aacgacttct gcggagtagt cgcacgttgc
    18181 cgctactttg gattttggcg caccttttgg catcaccact ttaccgtcga tacccagcat
    18241 cgcgcaggag agcgaaaccc cttgcgcatg gttgcccgca gaacaggcca ccacgccttt
    18301 gcgtttttcc gcatcggtca gtgaacttaa tttattaaat gcgccacgaa ttttaaatga
    18361 acccgtacgc tgcatatttt caaacttcag gaatatttca cctttgcaac gttcactaaa
    18421 atagttggag cgaggcatgc ctgttttata aattcgccca gccagtcgtt gtttcgcttc
    18481 aataatatca tcaatagcaa ccggcagatc gtatgtaata tgcattataa aacctcttcg
    18541 cctgaattaa atatgtaggt aaccgtaacc gacacctgca aaacagacag gtggattatt
    18601 tatgtttaaa taaaaacaaa caactaacca acttctatta attgccttcg tctacaccca
    18661 ttataagatg aatactcttt ggctaattcc accaaaaccg atgctgcttt tttaatacga
    18721 taatttttcg accataccgc ggcatattgt gccacaggta atgtttcttc aaccggaata
    18781 gtaataaatt gattagaacc aaaaggtgac gtcatatcac aaggaattac agttaagaaa
    18841 tcagcattga gaacaagatt ataaattgtc acgactgagt cggttttaac gatgttttca
    18901 atactgatgc catttctttg taacgtagta agcagttcgc tgtagtaccc catattagtt
    18961 tgtggcaaca cccactgttc gttcttcaac gactccagcg tggtggtgcc ggtgcatgtt
    19021 cgggacttac tggctaccag cacaaactcg gactcgaaca gcggctcaac atgtaaatcc
    19081 tgaagcttca tttctgcact taacgtacca atcgcaaaat ccagtcgacc gtcgcggatt
    19141 gccggtaaga acgaagacag ttgcgcttca tacatagaaa cctgcgcttt cgggaacacc
    19201 tctttgaact tgttgatcat ccctgacata aaagtaaaac caatcaatga aggaaaacca
    19261 aatgagactt ccaccaccgc ttcagaagac ataccgctta tctcattaac catatttttc
    19321 atttcacggg taatggattc ggaacgggag agtaacaatt gaccggcagg tgttaatgtt
    19381 acaccggtat ttttccgcac cactaattcc acaccaaaat aatcttcaat atcgttaatg
    19441 attttactga cggccggttg agttaaccct aattcttttg cagccgagcc gatagagcca
    19501 cttctaatga cttcctgaaa gactaccagg tgctgcgttt tcggaagaag aatagtgctc
    19561 ataacgacct acgttaatta cctcattgac ggcatgaagt gtatcaaaat gaaataaaca
    19621 ggatatgtgc gaccactcac aaattaactt tcattacttt ccaaggtatc gttattacaa
    19681 ttaaacatct ttaaatatca acaagttaaa gcataaaaat cagcataaac cctgattttt
    19741 atcaaaaaaa aatgacgggg ataaccatat tttatggcga taacatcatt cgttatgtgg
    19801 ttaatacaaa aaaggggctg agaccatatt tcaagcaatt accggataat taccaggcaa
    19861 aatttgagtt aaatttaatg agcaagttct caaattttat tataaataaa ccattttcag
    19921 cgataaatac tgcggcacgt cacatttttt cacgctattt gttggagaac aaacatttat
    19981 tttatcaata ttttaaaatt tcgaatacag gtattgatca tctcgaacaa ttgattaacg
    20041 tcaacttttt ctcttctgac aggacgtcat tttgtgaatg caatcgtttt ccataaattc
    20101 ttctcccctc ataggcgacg aatagcattt tgtgttgagg atcacaaaac gaataattgc
    20161 tggtcgccgc gataaggtca gacgaagtca acaaggggga ttttcactgt gctttacatc
    20221 agcgtaggac acagaatgtt gcttcgttta ctgtaacgcc gggtaaatga gcgttttttg
    20281 atagtgcgta agtgtcgtgc gaggaaaata agcatctatt attgtttgtc atgacgacaa
    20341 ggcaggcatc aatgaaaggc ttcccaattg cacatatttt tcacccttca atcccgccaa
    20401 tgcacgcagt ggttaacaat cacaatagaa atattgatta ttggacggta aaaagaaagt
    20461 ttgcagaaat tgtctccacc aatgacgtta ataaaattta cagtataagt aatgaactcc
    20521 ggagagtatt atctgcaatt actgcattga atttctatca aggcgatgtt ccttctgtca
    20581 tgattccgaa tccaaccgga aaatatgagt ccattcatca tagatatttc tacaggagaa
    20641 catgatgatt atatcataca aacattagat gtaggcactt ttgcaccttt tggtgaacaa
    20701 tgtacttgct cagccgtcaa taaaaaagag ctggaatgta ttaaggagac gatttctaag
    20761 tattgcgcaa agttcaccca aaaagaagcc atcttaacac cccctaggca ctttaataaa
    20821 acctgcatta cttcagattg ttggcaaatt ctcttttttt cacccgatca ttttaataat
    20881 gatttttatt gattatttat aaaggataaa ataatgttcc ctgtttcatc aattggtaat
    20941 gatattagta gtgatttagt ccgtcgaaaa atgaatgacc ttcctgaaag ccccatagga
    21001 aataacctcg aagcactagc accaggtata gaaaaactaa aacagacctc tattcaaatg
    21061 gtcactttac ttaacgcgtt acaaccaagt ggaaaatgca ttatcactgg tgattttcaa
    21121 aaagaattag cgtacttaca aaatgtaatt ctttataatg actcgtctct tcgtctggat
    21181 tttttaggtt ataacaccca aattattcaa cgatcggaca atacctgcga acttaccatt
    21241 aatgaaccgt taaaaaacta ggaaatatct acaggcaata tcaatgttaa ttgcccatta
    21301 aaagatattt acaatgaaat caggaggttg aacgtaatct ttagttgtgg gactggagat
    21361 atagttgatc tatcctctct ggacttacgt aatgtcgatt tagattatta tgatttcaca
    21421 gataaacata tggctaatac tattctaaat cattttaaat tggattcaac aaattttact
    21481 aatgccaaca tgtttcaggt taattttgtt agttcaacac aaaacgccac aatctcctgg
    21541 gattatttac taaaaataac gcctgtttta ataagcatta gcgatatgta ttctgaagaa
    21601 aaaatcaagt ttgtcgaaag ttgtttaaat gagcttggag acattaccga agaacaatta
    21661 aaaattatga gatttgcaat tataaaatct ataccaaggg caactcttac agataaatta
    21721 gaaaatgaat taacaaaaga aatatataaa agctcatcga aaatcaacaa ttgcttgaac
    21781 agaattaaat taccagagat gaaagaattc tcatcaaaaa aaacatatga ttacatcgat
    21841 acaatcattg aagattatga aaatatcaaa gaaaatgctt atctggtcgt cccccaaatt
    21901 aattatacta tggatttaaa catagaagac tctagctcag aagagttaat accctcgaga
    21961 aagacgaaaa ttctccggac aatggctttg aggtcgggga atataacaca tatgaagcat
    22021 ataactcaga gaatcaatat tttaccagag aggactatac gtataattac gaccttttaa
    22081 atgcaatata gacttaataa cttcttacaa agtctattat taataccgta gatattcatc
    22141 ttaccctgtg caccaaaagc aaaaacttat cgacgcgggt ttgaaactga tggcaaccgc
    22201 aaaaatgcct gatgcgctac gcttatcagg cctacgccat ctctgcaata tattgaattt
    22261 gcgtgctttt gtaggcagga taaggcgttc acgcagcatc cggcatgaat aaagcgcact
    22321 caacaatctg aaacccgccg gaacggttta ttacgtacat caggtgaaac tgaccgataa
    22381 gccgctttct tttgggtata gtgtcgtgga cagtcattca tctttctgcc cctccaaaag
    22441 caaaaacccg ccgaagcggg tttttacgta tatcaggtga aactgaccga taagccgctt
    22501 tcttttgggt atagcgtcgt ggacagtcat tcatctttct gcccctccaa aagcaaaaac
    22561 ccgccgaagc gggtttttac gtaaaacagg tgaaactgac cgataagccg ggttctgtcg
    22621 tggacagtca ttcatctagg ccagcaatcg ctcactggct caagcagcct acccgggttc
    22681 agtacgggcc gtaccttatg aacccctatt tggccttgct ccgggtggag tttaccgtgc
    22741 cacggactgt taccagccgc gcggtgcgct cttaccgcac cctttcaccc ttacctgatc
    22801 ccgcttgcac gggccatcgg cggtttgctc tctgttgcac tggtcgtggg tttccccccc
    22861 aggcgttacc tggcaccctg ccctatggag cccggacttt cctcccctcc acccgtctcc
    22921 cccgaagagg acgacgacga agcggcgact gtctggtcag cttcggcgcg cagtatagag
    22981 ggtttgcgcg cccttgtcac cccgtgttgc gcattccaat cgccagtgtc gcggcaatat
    23041 tacgtgaagc acggcagata ttgtcataag ccccgcggaa tgcttcgtcc aacgtaccta
    23101 tgctggtcaa tacgctgaag accgcatcaa tgccatgctg atgtacaacg ccaacatcat
    23161 cggtcaggct acccgcaatg ccaatcaccg gtttatggta cttcttcgcc acgtttgcga
    23221 cgccaatcgg taccttgccg tgaatactct ggctgtcaat acgcccttca ccggtgatca
    23281 ccagcgtaca atcgtgaata tgttcctcca gattcagcgc cgtagtgacg atttcaatac
    23341 cacttttcag ttccgcacca agaaacgcca tcagcgccgc acccataccg cccgcagctc
    23401 ctgcgccggg gacatctttt acatcaacat gcagcgcttt tttaatgaca tcggcatagt
    23461 gagagaggtt attgtccagc tcaacaatca tcgcttcact ggctcccttt tgtgggccaa
    23521 agatgcgcga tgcgccgtta tcgcccacca gcggattggt gacatcacaa gcgacgcgaa
    23581 tgacgcaatc ttttaagcgc ggatcgaggc cggaaatatc aatatcattc agagtattaa
    23641 gactaccgcc gccaaaacca atttcattgc cgttggcgtc gcataatttc gcccccagcg
    23701 cctgtaccat gcctgcgccg ccatcatttg tagcgctacc gccaatgccg ataataatgt
    23761 ttgtcgcgcc gctctccagc gcctgcagga ttaactcgcc tgtgccgcgt gaagtggtca
    23821 cgagtggatc gcgtttttcc gcaggtacca gctccagccc actggccgcc gccatttcaa
    23881 taaacgcggt tttgccatcg ccggagatcc cccaactggc attcactttc tcgcccagcg
    23941 gccctgtaac ccaggcgtga cgttcagaac cctgggtggc tgcaatcatc gcttccaccg
    24001 ttccttcgcc accgtcggca accggaatag aaacgtactg tgcatcagga aaaatttccc
    24061 gaaatccttt ttctatcgcc tgcgcaacct cgctggcaga taaactttct ttataagagt
    24121 ctggggcgat tacgattttc atacctatgc ctgttaccac atgacgccgg agggcgtttc
    24181 tcttattcgg cctggattcc aggcccggat tgcaatacgc catccgggca cgacgtcatt
    24241 aacgagtaac ttcgactttc gccagttttt cgtagtagca caccagggcg ctatgatccg
    24301 ccgttcctaa accatctgct cgcagtgcct gcatcatctc cataaccgca gctgtgagcg
    24361 gcagttgcgc gccgacgccg tgggaagtat ccagcgcatt tgccagatcc ttaatatgca
    24421 gatcaatacg gaagcccggc ttgaagttgc ggtccatcac catcggcgct ttggcatcca
    24481 gcacggtact gcccgccagt ccaccgcgaa ttgcctgata caccaggtcc gggttaacgc
    24541 ccgctttagt tgccagcgtt aacgcttctg acatcgcggc aatattcagc gccacaatga
    24601 cctgattcgc cagtttggtg acgttacctg caccaattgc cccggtatgc accacggaac
    24661 ccgccattgc tttcatcaaa tcatagtatt tgtcgaaaat agccttgtcg ccgcccacca
    24721 tcactgacag cgtaccgtcg atggctttcg gttcaccgcc gctcaccgga gcatccagca
    24781 tatcaatgcc tttcgctttc agcgcttcgc tgatttcacg gcttgccagc ggtgcgatag
    24841 aactcatatc gatcaatacc gtacctggct tcgcgccttc aataatgcca ttctcaccca
    24901 gcgccacctc tttcacatga ggggagtttg gcagcatggt tatgatgacg tcgcactgtt
    24961 cagcgatcgc tttagccgta gaggctgttt ctgcacctgc agcaatcacg tcagcaatag
    25021 cttctgggtt acggtcagca accaccagcg agtaacctgc tttcagaagg tttttactca
    25081 ttggtttacc cataatcccc aggccaataa aaccaacttt catagtcata tcaatcatct
    25141 ctcttgttgc ggtggtggtt attttttaaa ggtatcagcc agtttatgag tggcagagcg
    25201 gaagacgccg agatcgctgc cgacagccac aaacgtcgcg ccccattcca gataacgacg
    25261 cgcatcggct tcgaccggcg cgaggatacc gctgggtttg ccgtgcgcgc tggcacggtt
    25321 aaaaatgtgc tgaattgctt tttgtacatc cgggtgtgat gcattgccga gatggcctaa
    25381 tgccgcggcc agatcgctgg ggccgacgaa gatgccgtct acgccttcgg tagcggcaat
    25441 ggcatcgacg ttatctacgc cctgctgact ttctatctgg accagaatag tgatgttctt
    25501 gttcgactga gcgaaataat ccgccacggt gccaaacata ttggcgcggt gagaaacgga
    25561 gacgccgcga atgccttccg gtgggtaacg ggttgatgcc accgcctgct ctgcttcctc
    25621 ttttgtttct acaaaaggaa tcaggaagtt atagaaaccg atatccagaa gacgcttaat
    25681 aatttccggc tcgttggtcg gcactcacac tactggcgcg ctggcgctgc ctttcaaggc
    25741 cattaactgc ggaataaacg tggagatatc gtttggcgca tgttcgccat ccagcaccag
    25801 ccagtcaaac ccagccaaac caagaacttc agtgctaatc gggttagaga gtgctgacca
    25861 gcaaccaatt tgtacctgtt tcgcagccag tgcggctttg aatttattcg ggaaaacatc
    25921 gttattcatc gcttatacct ttgcttattt ctgcaattcc atacgtttaa tgtcgccaac
    25981 tacgaagagg tagcagacca tcgccatcag cgctgaacat cccacgaaaa ccagtgctgc
    26041 attgaaggag tgcagttcac ttaccaggta gccaatcacc agtggagtga caatggaggc
    26101 aacattgcca aagacgttaa agacgccgcc acagaggcca acaatctctt tcggcgcggt
    26161 gtcagaaatc accggccagc ccagagcacc aaatcctttg ccaaaaaaag ccagcgccat
    26221 cagcatgacc accagcgtgg tgttgttggt gtagttacat aagatgatgg tggaagccag
    26281 caacattccc agcacaatcg gtagcttacg tgccagggtc agggataaac cgcgtttgat
    26341 cagataatcc gagaagacac ctcccagcac gccgcccgca aaaccacaca gtgccggaat
    26401 cgaggcgacc agacccactt tcagaatcga catgcctttt tcctgcacca gataaatcgg
    26461 gaaccaggtg aggaagaacc aggtgatggt gttgataaaa tattgtccga aaaatacgcc
    26521 caacatcatg cggttagaga gcaattgctt gatgtaatgc agtttgggac cgcttgctgc
    26581 cgcgctgccc ggctttttgt ggtccatatc gaccaccgcg ccattttcag agataaactt
    26641 cagctcttcc tcagacatac gtgggtgatc tgtcgggtta tgaatcaact tgatccacag
    26701 cgccgtcagc acaaaacaaa tcacccccat aacggtaaag acgtgctccc atccccaggc
    26761 gaaagtcagc cagccaagca gaggcgaaaa gagcgccagc gagaaatatt gtgccgagtt
    26821 aaagatggcg gaggcagtac cacgttcttt cgtcgggaac caggcggcga caatacgggc
    26881 gttcgccggg aaggatggag cttccgagaa tcccagcata aagcgcataa agaacatgga
    26941 gatccctgcc caagccagcg ggaacatatc aacaaagcct tgcaggaagg tgaacagcga
    27001 ccagaaaaag aggctgtagg tgtaaacttt tttcgagcca aacttatcaa gcaaccagcc
    27061 gccggggatt tgcatcagca agtaggccca gccaaaagcg gagaagatgt aacccatcga
    27121 aaccgcactt aactgcaact cttttgccac ttcggtacca gcaatagaca gcgttgcacg
    27181 atcggcgtag ttaacggcgg taacaataaa aataatcagt aatattaaat agcgggtatg
    27241 cacacctttc cttttttcgt caacggtgtc cagaatcatt ttatttacct cgggtactta
    27301 tgctgatttt tattattatg gggaaggtgt tatttataag tttcatttat gccgtaacga
    27361 caatgaactc gggaattagt ataagcagcg cgagaataat aatcattgtg caaatgctaa
    27421 tttaattaat actttttaaa tattattttg agcatatgca cataaggttg cgcgctaaag
    27481 cacagatttg cgctttacct tgccgggcgg cactgcaatc cctaaaatga ttgacattga
    27541 tcacatttct gcgtttaaac tcctgacatt cttatttcac ccaatgaaat catttatttt
    27601 taaatgagac caggtcctca ttttaataac ccctggctgg agaatattgc acaatggcca
    27661 acatcgaaat cagacaagaa acgccaactg cgttttatat aaaagttcac gacacagata
    27721 atgtggcaat tattgttaat gataatggcc tgaaagcagg aacgcgtttt ccggatgggc
    27781 tggaattaat tgaacatatt ccccaggggc ataaagtcgc attgctggac attccggcta
    27841 atggtgaaat tattcgttat ggcgaagtga ttggttacgc cgtgcgtgca atcccacgcg
    27901 gaagctggat cgacgaatca atggttgtac taccggaagc gccgccgtta cacacgctgc
    27961 cactggcaac caaagtcccg gaacccttac cgccgctgga aggatacacc tttgagggct
    28021 atcgcaatgc cgatggcagc gtgggcacca aaaacctgct cggcatcacc accagcgtcc
    28081 actgtgtggc aggcgtggtg gactatgtag taaaaatcat tgaacgcgat ctgctaccga
    28141 aatacccgaa cgtcgatggc gtggtggggc tgaatcattt gtacggttgt ggcgtggcga
    28201 ttaacgcacc ggcagcagtt gtacctattc gtaccattca caatatttcg ctgaacccta
    28261 actttggcgg cgaagtaatg gtgattggcc tgggttgtga aaagttgcag cctgagcgcc
    28321 tgctgactgg aacggatgat gtgcaagcta ttccagtaga aagcgccagc attgtcagtt
    28381 tgcaggatga aaagcatgtc ggttttcagt ccatggtcga ggatattttg caggtcgccg
    28441 aacgccatct acaaaaactg aatcaacgtc agcgagaaac ctgtccggct tcagaactgg
    28501 ttgtcggcat gcattgcggt ggcagcgatg cgttttctgg cgtaacggca aacccggcgg
    28561 ttggctatgc gtctgatcta ctggtgcgct gcggcgcaac ggtgatgttt tcagaagtca
    28621 cggaagtgcg tgacgcgatc catctgctga caccacgcgc agtgaacgaa gaggtcggca
    28681 aacggctgct ggaggagatg gagtggtacg ataactatct caatatggga aaaaccgacc
    28741 gcagcgccaa cccttcgccg ggcaacaaga aaggcggtct agcaaacgtg gtagagaaag
    28801 cgctcggctc cattgctaaa tcgggcaaaa gtgcaattgt tgaagtgctg tcgcccggtc
    28861 aacgcccgac taaacgcgga ttaatttacg ccgcgacgcc agccagcgat tttgtctgtg
    28921 gcacgcaaca ggtggcttcg ggtatcaccg tgcaagtgtt tacgaccggt cgtggtacgc
    28981 cgtacggcct gatggcggta cccgtcatta aaatggcacc ccgcaccgag ctggcgaacc
    29041 gctggtttga tttaatggat attaatgcgg gcaccatcgc taccggcgaa gaaactattg
    29101 aagaggaggg ctggaagttg ttccacttta ttctcgacgt cgccagcggg aagaagaaaa
    29161 ccttctcgga tcaatgggga ctgcataacc agctggcggt gtttaaccca gcaccggtga
    29221 cctgataatt caattcgtat tatgcccgat agcacacgtt tatcgggcat attgagtcac
    29281 agcgaagacg cgtgatcccc gaccagaatc acttcaaccc cagcctttcg cagtccttcc
    29341 agactatccg caggaatgcc ttcatcaaca atgatcatgt cgatacgttg agtatcaatg
    29401 atcttatgta aactggaacg attgaattta ctggaatcgg tgaccacgat gatccgttcc
    29461 gcaacttcgc acatccgacg gcttaaacgg gcttcatctt cattatgtgt gctgacgccg
    29521 cgctccagat cgatcgcatc tacaccaaga aacagcatat cgaagtggta attttgcagc
    29581 gattgctcag cctgatcgcc gtaaaaagat tgcgactgac ggcgcaaatg cccgccggtc
    29641 atcagcagct caacgccttc cgcttccagc aacgcattag ccacgttcat accgttggtc
    29701 atcgcaatta cgtcagtgta cttgcgcatc agacgagcaa tctcaaaagt ggtggtcccg
    29761 gaatcgagga tcacccgatg acctggctga atcaactcaa cggcagcttt cgcaacgctg
    29821 cgtttcatcg cggtgttcag tgcgctttta tcttccactg atggctcgac tgacggcgtc
    29881 gtgctatcgc agatcaacgc gccaccatag gcacgcacag cgatcccctg cttttccaga
    29941 aacgccagat cattgcggat cgtcacagta gatacgccat acaatgccga cagatcgtta
    30001 acctgcacac tcccttgctg tcgcagacgc tgaatgatct gttctcgtcg ctcgctggtg
    30061 cctgtcactc gcttctcacc tgaagcgtcg gtattactca tagtaagtcc tttcgtaaaa
    30121 ctttcgtttc atttcgtttt gcctattaac gcctttctat taagcaaatg caagcccacc
    30181 ttgcccattg gcgcaagcta ctctcgtttc agtgactttc attacgtttc ttttgtgaat
    30241 cagatcagaa aactattatc tttcgtttta tttttatctc accatgacgc agtatcaatt
    30301 gaaacaaaac gaaagatcaa tatcgcagta atctgaactg gagaggaaag tgaaacatct
    30361 gacagaaatg gtgagacagc acaaagcggg caaaacaaat gcaatttatg ccgtttgttc
    30421 cgcacatccg ctggtgctgg aggctgcaat ccgctacgcc agtgcaaacc aaacgccgtt
    30481 actgattgaa gcaacctcca atcaggtaga ccagttcggc ggttataccg gaatgacgcc
    30541 cgccgatttt cgcggctttg tttgtcagct cgccgactcg ttgaatttcc cgcaggatgc
    30601 gctgattctg ggtggtgacc atctggggcc aaaccgctgg caaaacctgc cagccgctca
    30661 ggcaatggcc aatgccgatg atttgattaa aagctacgtt gcggcaggat tcaaaaaaat
    30721 ccaccttgat tgcagcatgt cctgtcagga cgatccaatt cccttaactg atgacatcgt
    30781 ggctgaacgc gccgcccgtc tggcgaaagt ggcggaagaa acctgtcgcg aacactttgg
    30841 cgaagccgat ctggagtatg tcattggtac cgaagtgccg gtacctggcg gcgcgcatga
    30901 aaccttaagc gagctggcgg tcaccacgcc ggatgccgcc cgcgccacgc tggaagccca
    30961 tcgtcacgcc tttgaaaagc aaggtttgaa tgccatctgg ccacgcatta ttgccctggt
    31021 ggttcaaccc ggcgtcgaat tcgatcacac caacgttatt gattatcagc ccgccaaagc
    31081 gagcgcctta agccagatgg tcgaaaacta cgaaacgctg attttcgaag cgcactctac
    31141 cgattatcaa acgccgcaat ctctgcgcca gctggtgatt gaccactttg ccattctgaa
    31201 agttggcccg gcgctgacct tcgccctgcg tgaagccctt ttttctctgg cggcgattga
    31261 agaagaactg gtaccagcga aagcctgttc cggtctgcgt caggtgctgg aaaacgtgat
    31321 gctcgaccgc ccggaatact ggcaaagcca ctaccacggt gacggcaacg cgcgtcgtct
    31381 ggcgcgtggt tacagctact cagatcgcgt gcgctattac tggccggaca gccagattga
    31441 tgacgctttc gctcatctgg ttcgtaatct ggcggattca ccaattccgc taccgctcat
    31501 cagccagtat ctgccgctgc agtacgtgaa agttcgctcc ggcgagctgc aaccgacgcc
    31561 acgggaactc attataaacc atattcagga catcctggcg cagtaccaca cagcctgtga
    31621 aggccaataa gcaaaacaaa gaggaacatg ctatgccaaa tattgtttta agccggattg
    31681 atgaacgctt gattcacggt caggtcggcg ttcagtgggt cggatttgcg ggggcaaatc
    31741 tggtgctggt agccaacgat gaggttgccg aagatccggt acaacaaaac ctgatggaaa
    31801 tggtactggc agaagggatc gccgtacgtt tctggacgct gcaaaaagtt atcgacaaca
    31861 ttcatcgcgc cgccgatcga cagaaaatcc tgctggtttg taaaacaccc gccgatttcc
    31921 tgacgctggt gaaaggtggc gttccggtga atcgcattaa cgttggcaat atgcactacg
    31981 ccaatggcaa acaacaaatc gccaaaacgg tttctgtgga tgcgggcgat atcgcagcat
    32041 ttaacgacct gaaagccgct ggggtggaat gcttcgttca gggcgtcccg acagagcctg
    32101 ctgtggacct ctttaaatta ctttgaggga ttcatcatgg aaatcagcct gttgcaggca
    32161 tttgcgttgg gcattatcgc ctttatcgct ggcctagata tgtttaacgg cttaacccat
    32221 atgcaccgcc cggtggtcct cggcccgttg gtcgggctgg tacttggcga tctgcatacc
    32281 ggaattttaa ccggcgatac gctggaacta gtgtggatgg ggctggcccc gctggcgggc
    32341 gcacagccgc ctaacgtgat tatcggtact atcgtcggca cggcgtttgc cattactact
    32401 ggcgtgaaac ccgatgtcgc ggtcggtgtc gccgtacctt tcgctgtcgc agtacagatg
    32461 gggattacct tcctgttctc ggtgatgtct ggcgtgatgt ctcgctgcga ccggatggcg
    32521 gagaacgccg atactcgcgg cattgaacgc gtgaactatc tggcactgct ggcactcggc
    32581 actttctatt ttctctgcgc tttcctgcct atctacttcg gcgcggaaca tgcaaaaacc
    32641 atcattgatg tcctgccgca acgattaatc gacggcctcg gcgtcgcggg cggcatcatg
    32701 ccagcaatcg gttttgccgt gctgctgaaa atcatgatga aaaacgtcta cataccctac
    32761 ttcatcctgg gctttgttgc cgcaacctgg ctcaagttac cggtgctggc tattgctgcc
    32821 gccgcgctgg cgatggcgct gatcgatctg ctgcgtaaat ctcctgaacc gactcaacct
    32881 gcggcacaga aagaggaatt tgaagatggc atctaatcaa accaccctgc cgaacgtcag
    32941 cgaaaatgaa gaaacactgc tgactggcgt caatgaaaac gtgtatgaag atcggagcat
    33001 tgccgcggag ctgacgaaaa aagatatcaa ccgcgtcgcc tggcgttcca tgctgttaca
    33061 agcgtctttt aactacgaac gtatgcaggc ttctggctgg ttgtacggtc tgctgcctgc
    33121 tctgaaaaag atccacacta ataaacgcga cctggcgcgc gccatgaagg ggcatatggg
    33181 tttcttcaat acccatccgt ttctggtgac gtttgttatc ggcattatcc ttgcgatgga
    33241 gcgttctaag caggacgtta acagtattca gagcaccaaa attgccgtcg gtgcgccgct
    33301 tggcgggatt ggcgatgcga tgttctggct aacgctactg ccgatttgtg gcgggatagg
    33361 cgcaagcctc gcgctacaag gctctattct tggcgcagtc gtctttattg tgctgttcaa
    33421 cgtggtacac ttggggctgc gttttggtct ggcgcattat gcttaccgca tgggcgtggc
    33481 ggcgattccc cttattaaag cgaataccaa aaaagtcggc catgctgcgt caatcgttgg
    33541 aatgacggta atcggcgcac tggtggcaac ctatgttcgt ttaagcacca cgctggaaat
    33601 cacggcgggc gacgcagtag ttaagttaca ggctgatgtt atcgacaaac tgatgcctgc
    33661 cttcttaccg ctggtctaca ccctgaccat gttctggctg gtacgccgcg gctagagtcc
    33721 gctgcgcctg attgcggtta ccgtggttct agggatcgtc ggtaaattct gccatttcct
    33781 ttaaatacaa tgaggtatta gatgttaagt attattttga cagggcatgg cgggtttgcc
    33841 agtggcatgg aaaaagcaat gaagcaaatc ctcggcgatc aatcgcagtt tattgccatc
    33901 gattttcccg aaacctcaag cgccgcgctg cttacatcgc agcttgaaga ggccatcgcc
    33961 caactggatt gtgaagacgg cattgttttt ctgacggatt tgctgggcgg cacgccgttt
    34021 cgcgtagctt caacgctggc gatgcaaaaa ccgggctgtg aagtgattac cggcaccaat
    34081 ttgcaactgc tactggagat ggtgctggag cgcgaagggt taagcggtga agaattccgt
    34141 gtacaggcgc tggagtgtgg acaccgaggg ctgaccagtc tggtggacga gttaggccgc
    34201 tgccatgaag aatgtccggt cgaggaagga atatgacaca cgttctgcgc gccagaaggc
    34261 tgctgactga agagggatgg ttcgatgacc atcagttgcg tgttgctgac ggtgtcatcg
    34321 cagcaatcga accgatt
//
LOCUS       DAJTRC010000037        33913 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.037, whole genome shotgun sequence.
ACCESSION   DAJTRC010000037 DAJTRC010000000
VERSION     DAJTRC010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.037"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            87..173
                     /locus_tag="OO357_001886"
                     /pseudo
     CDS             87..173
                     /locus_tag="OO357_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015869530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(578..1054)
                     /locus_tag="OO357_001887"
     CDS             complement(578..1054)
                     /locus_tag="OO357_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase inhibitor"
                     /protein_id="HCR5886848.1"
                     /translation="MKLISNDLRDGDKLPHRHVFNGMGYDGDNISPHLAWDDVPAGTK
                     SFVVTCYDPDAPTGSGWWHWVVVNLPADTRVLPQGFGSGLVAMPDGVLQTRTDFGKTG
                     YDGAAPPKGETHRYIFTVHALDVERIDVDEGASGAMVGFNVHFHSLASASITAMFS"
     gene            complement(1113..2402)
                     /gene="bioA"
                     /locus_tag="OO357_001888"
     CDS             complement(1113..2402)
                     /gene="bioA"
                     /locus_tag="OO357_001888"
                     /EC_number="2.6.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylmethionine--8-amino-7-oxononanoate
                     transaminase"
                     /protein_id="HCR5886849.1"
                     /translation="MTTDDLAFDQRHIWHPYTSMTSPLPVYPVASAEGCELILSDGRR
                     LVDGMSSWWAAIHGYNHPQLNAAMKTQIDAMSHVMFGGITHAPAIELCRKLVAMTPQP
                     LECVFLADSGSVAVEVAMKMALQYWQAKGEARQRFLTFRNGYHGDTFGAMSVCDPDNS
                     MHSLWKGYLPENLFAPAPQSRMDGEWDERDMVGFARLMAAHRHEIAAVIIEPIVQGAG
                     GMRMYHPEWLKRIRKMCDREGILLIADEIATGFGRTGKLFACEYAEIAPDILCLGKAL
                     TGGTMTLSATLTTREVAETISNGEAGCFMHGPTFMGNPLACAAANASLAIIESGEWQQ
                     QVAAIEVQLREQLAPARDAEMVADVRVLGAIGVVETTRPVNMAALQKFFVEQGVWIRP
                     FGKLIYLMPPYIILPQQLQRLTAAVNRAVQDETFFCQ"
     gene            2489..3529
                     /gene="bioB"
                     /locus_tag="OO357_001889"
     CDS             2489..3529
                     /gene="bioB"
                     /locus_tag="OO357_001889"
                     /EC_number="2.8.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin synthase BioB"
                     /protein_id="HCR5886850.1"
                     /translation="MAHRPRWTLSQVTELFEKPLLDLLFEAQQVHRQHFEPRQVQVST
                     LLSIKTGACPEDCKYCPQSSRYKTGLEAERLMEVEQVLESARKAKAAGSTRFCMGAAW
                     KNPHERDMPYLEQMVQGVKAMGLEACMTLGTLSESQAQRLANAGLDYYNHNLDTSPEF
                     YGNIITTRTYQERLDTLEKVRDAGIKVCSGGIVGLGETVKDRAGLLLQLANLPTPPES
                     VPINMLVKVKGTPLADNDDVDAFDFIRTIAVARIMMPTSYVRLSAGREQMNEQTQAMC
                     FMAGANSIFYGCKLLTTPNPEEDKDLQLFRKLGLNPQQTAVLAGDNEQQQRLGQALMT
                     PDTDEYYNAAAL"
     gene            3526..4680
                     /gene="bioF"
                     /locus_tag="OO357_001890"
     CDS             3526..4680
                     /gene="bioF"
                     /locus_tag="OO357_001890"
                     /EC_number="2.3.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="8-amino-7-oxononanoate synthase"
                     /protein_id="HCR5886851.1"
                     /translation="MSWQEKINAALDARRAADALRRRYPVAQGAGRWLVADDRQYLNF
                     SSNDYLGLSHHPQIIRAWKQGAEQFGIGSGGSGHVSGYSVVHQALEEELAEWLGYSRA
                     LLFISGFAANQAVIAAMMAKEDRIVADRLSHASLLEAASLSPSQLRRFTHNDVAHLAR
                     LLASPCPGQQLVVTEGVFSMDGDSAPLAEIQQVTQQHNGWLMVDDAHGTGVIGEQGRG
                     SCWLQKVKPELLVVTFGKGFGVSGAAVLCSSTVADYLLQFARHLIYSTSMPPAQAQAL
                     RASLAVIRRDEGDARREKLVSLIARFRAGVQDLPFTLADSCSAIQPLIVGDNSRALQL
                     AEKLRQQGCWVTAIRPPTVPAGTARLRLTLTAAHEMQDIDRLLEVLHGNG"
     gene            4667..5422
                     /gene="bioC"
                     /locus_tag="OO357_001891"
     CDS             4667..5422
                     /gene="bioC"
                     /locus_tag="OO357_001891"
                     /EC_number="2.1.1.197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonyl-ACP O-methyltransferase BioC"
                     /protein_id="HCR5886852.1"
                     /translation="MATVNKQAIAAAFGRAAAHYEQHADLQRQSADALLAMLPQRKYT
                     HVLDAGCGPGWMSRHWRERHAQVTALDLSPPMLAQARHKDAADHYLAGDIESLPLATA
                     TFDLAWSNLAVQWCGNLSTALRELYRVVRPGGVVAFTTLVQGSLPELHQARQAVDERP
                     HANRFLPPDEIEESLNGVRYQHHIQLITLWFDDALSAMRSLKGIGATHLHEGRDPRIL
                     TRSQLQRLQLAWPQQQGRYPLTYHLFLGVIARE"
     gene            5415..6092
                     /gene="bioD"
                     /locus_tag="OO357_001892"
     CDS             5415..6092
                     /gene="bioD"
                     /locus_tag="OO357_001892"
                     /EC_number="6.3.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706656.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent dethiobiotin synthetase BioD"
                     /protein_id="HCR5886853.1"
                     /translation="MSKRYFVTGTDTEVGKTVASCALLQAAKAVGYRTAGYKPVASGS
                     EKTPEGLRNSDALALQHNSSLQLDYATVNPYTFAESTSPHIISAQEGRPIESLVMSAG
                     LRALEQQADWVLVEGAGGWFTPLSDTFTFADWVTQEQLPVILVVGVKLGCINHAMLTA
                     QAIQHAGLTLAGWVANDVTPPGKRHAEYMTTLTRMIPAPLLGEIPWLAENPENAATGK
                     YINLALL"
     gene            6671..8692
                     /gene="uvrB"
                     /locus_tag="OO357_001893"
     CDS             6671..8692
                     /gene="uvrB"
                     /locus_tag="OO357_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit B"
                     /protein_id="HCR5886854.1"
                     /translation="MSKPFKLNSAFKPSGDQPEAIRRLEEGLEDGLAHQTLLGVTGSG
                     KTFTIANVIADLQRPTMVLAPNKTLAAQLYGEMKEFFPENAVEYFVSYYDYYQPEAYV
                     PSSDTFIEKDASVNEHIEQMRLSATKAMLERRDVIVVASVSAIYGLGDPDLYLKMMLH
                     LTVGMIIDQRAILRRLAELQYARNDQAFQRGTFRVRGEVIDIFPAESDDIALRVELFD
                     EEVERLSLFDPLTGQIVSTIPRFTIYPKTHYVTPRERIVQAMEEIKEELAARRKVLLE
                     NNKLLEEQRLTQRTQFDLEMMDELGYCSGIENYSRFLSGRGPGEPPPTLFDYLPADGL
                     LVVDESHVTIPQIGGMYRGDRARKETLVEYGFRLPSALDNRPLKFEEFEALAPQTIYV
                     SATPGNYELEKSGGDVVDQVVRPTGLLDPIIEVRPVATQVDDLLSEIRQRAAINERVL
                     VTTLTKRMAEDLTEYLEEHGERVRYLHSDIDTVERMEIIRDLRLGEFDVLVGINLLRE
                     GLDMPEVSLVAILDADKEGFLRSERSLIQTIGRAARNVNGKAILYGDRSTPSMAKAIG
                     ETERRREKQQKYNEEHGITPQGLNKKVVDILALGQNIAKTKAKGRGKSRPIVEPDNVP
                     MDMLPKALQQKIHELEGLMMQHAQNLEFEEAAQIRDQLHQLRELFIAAS"
     gene            complement(8884..9792)
                     /gene="yvcK"
                     /locus_tag="OO357_001894"
     CDS             complement(8884..9792)
                     /gene="yvcK"
                     /locus_tag="OO357_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706658.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine diphosphate-N-acetylglucosamine-binding
                     protein YvcK"
                     /protein_id="HCR5886855.1"
                     /translation="MRNRTLADLDRVVALGGGHGLGRVLSSLSSLGSRLTGIVTTTDN
                     GGSTGRIRRSEGGIAWGDMRNCLNQLITEPNVASAMFEYRFGGNGELSGHNLGNLMLK
                     ALDHLSVRPLEAINLIRNLLKVDTHLIPMSEHPVDLMAIDDQGHEVYGEVNIDQLTTP
                     IQELLLTPNVPATREAVHAINEADLIIIGPGSFYTSLMPILLLKEIAQALRRTPAPMV
                     YIGNLGRELSLPAANLKLESKLAIMEQYVGKKVIDAVIVGPKVDVSAVKERIVIQEVL
                     EASDIPYRHDRQLLHNALEKALQALG"
     regulatory      10057..10201
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01055"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="molybdenum cofactor riboswitch; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="molybdenum cofactor"
                     /db_xref="RFAM:RF01055"
     gene            10189..11178
                     /gene="moaA"
                     /locus_tag="OO357_001895"
     CDS             10189..11178
                     /gene="moaA"
                     /locus_tag="OO357_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706659.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP 3',8-cyclase MoaA"
                     /protein_id="HCR5886856.1"
                     /translation="MASQLTDVFARKFYYLRLSITDVCNFRCTYCLPDGYKPSGVTNK
                     GFLTVDEIRRVTRAFASLGTEKVRLTGGEPSLRRDFTDIIAAVRENDAIRQIAVTTNG
                     YRLERDVANWRDAGLTGINVSVDSLDARQFHAITGQDKFNQVMAGIDAAFEAGFEKVK
                     VNTVLMRDVNHHQLDTFLNWIQHRPIQLRFIELMETGEGSELFRKHHISGQVLRDELL
                     RRGWIHQLRQRSDGPAQVFCHPDYAGEIGLIMPYEKDFCATCNRLRVSSIGKLHLCLF
                     GEGGVNLRDLLEGDTQQQALEARISAALREKKQTHFLHQNNTGITQNLSYIGG"
     gene            11200..11712
                     /gene="moaB"
                     /locus_tag="OO357_001896"
     CDS             11200..11712
                     /gene="moaB"
                     /locus_tag="OO357_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein B"
                     /protein_id="HCR5886857.1"
                     /translation="MSQVSTEFIPTRIAILTVSNRRGEEDDTSGHYLRDSAQEAGHHV
                     VDEAIVKENRYAIRAQVSAWIASDDVQVVLITGGTGLTEGDQAPEALLPLFDREVEGF
                     GEVFRMLSFEEIGTSTLQSRAVAGVANKTLIFAMPGSTKACRTAWENIIAPQLDARTR
                     PCNFHPHLKK"
     gene            11715..12200
                     /gene="moaC"
                     /locus_tag="OO357_001897"
     CDS             11715..12200
                     /gene="moaC"
                     /locus_tag="OO357_001897"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HCR5886858.1"
                     /translation="MSQLTHINAAGEAHMVDVSAKAETVREARAEAFVTMRSETLAMI
                     IDGRHHKGDVFATARIAGIQAAKRTWDLIPLCHPLMLSKVEVNLQAEPEHNRVRIETL
                     CRLTGKTGVEMEALTAASVAALTIYDMCKAVQKDMVIGPVRLLAKSGGKSGDFKVEAD
                     D"
     gene            12193..12438
                     /gene="moaD"
                     /locus_tag="OO357_001898"
     CDS             12193..12438
                     /gene="moaD"
                     /locus_tag="OO357_001898"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706662.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase sulfur carrier subunit"
                     /protein_id="HCR5886859.1"
                     /translation="MIKVLFFAQVRELVGTDATEVAADYPTVEALRQHLAAQSDRWAL
                     ALEDGKLLAAVNQTLVSFDHPLTDGDEVAFFPPVTGG"
     gene            12440..12892
                     /gene="moaE"
                     /locus_tag="OO357_001899"
     CDS             12440..12892
                     /gene="moaE"
                     /locus_tag="OO357_001899"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin synthase catalytic subunit MoaE"
                     /protein_id="HCR5886860.1"
                     /translation="MAETKIVVGPQPFSVGEEYPWLAERDEDGAVVTFTGKVRNHNLG
                     DSVKALTLEHYPGMTEKALAEIVDEARNRWPLGRVTVIHRIGELWPGDEIVFVGVTSA
                     HRSSAFEAGQFIMDYLKTRAPFWKREATPEGDRWVEARESDLQAAKRW"
     gene            13029..13733
                     /locus_tag="OO357_001900"
     CDS             13029..13733
                     /locus_tag="OO357_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5886861.1"
                     /translation="MDRFPRSDSIVQPWAGLQTYMAQVYGWMTVGLLLTAFVAWYAAN
                     SAAVMELLFTNRVFLIGLIIAQLALVIVLSAMIQKLSAGVTTMLFMLYSALTGLTLSS
                     IFIVYTAASIASTFVVTAGMFGAMSLYGYTTKRDLSGFGNMLFMALIGIVLASLVNFW
                     LKSEALMWAVTYIGVIVFVGLTAYDTQKLKNMGEQIDTRDTSNLRKYSILGALTLYLD
                     FINLFLMLLRIFGNRR"
     gene            13938..14651
                     /locus_tag="OO357_001901"
     CDS             13938..14651
                     /locus_tag="OO357_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HCR5886862.1"
                     /translation="MESYSQNSNKLDFQHEARILNGIWLITALGLVATAGLAWGAKYL
                     EITATKYDSPPMYVAIGLLLLCMYGLSKDINKINAAIAGVIYLFLLSLVAIVVASLVP
                     VYAIIIVFSTAGAMFLISMLAGLLFNVDPGSHRFIIMMTLTGLALVIIVNAALMSERP
                     IWVISCLMIVLWPGIISHGRNKLLELAGKCHSEELWSPVRCAFTGALTLYYYFIGFFG
                     ILAAIAITLVWQRHTRFFH"
     gene            complement(14687..15643)
                     /locus_tag="OO357_001902"
     CDS             complement(14687..15643)
                     /locus_tag="OO357_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HCR5886863.1"
                     /translation="MSKSHPRWRLAKKILTWLFFIAVIVLLVVYAKKVDWEEVWKVIR
                     DYNRVALLSAVGLVVVSYLIYGCYDLLARFYCGHKLAKRQVMLVSFICYAFNLTLSTW
                     VGGIGMRYRLYSRLGLPGSTITRISSLSITTNWLGYILLAGIIFTAGVVELPDYWYVD
                     QTTLRILGIGLLMIIAVYLWFCAFAKHRHMTIKGQKLVLPSWKFALAQMLISSVNWMV
                     MGAIIWLLLGQSVNYFFVLGVLLVSSIAGVIVHIPAGIGVLEAVFIALLAGEHTSKGS
                     IIAALLAYRVLYYFIPLLLALICYLLLESQAKKLRAKNEAAM"
     gene            complement(15643..16884)
                     /gene="clsB"
                     /locus_tag="OO357_001903"
     CDS             complement(15643..16884)
                     /gene="clsB"
                     /locus_tag="OO357_001903"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsB"
                     /protein_id="HCR5886864.1"
                     /translation="MKCSWREGNKIQLLENGEQYYPAVFKAIGEAQERIILETFIWFE
                     DNVGKQLHAALLAAAQRGVKAEVLLDGYGSPDLSDEFVNELTAAGVVFRYYDPRPRLF
                     GMRTNVFRRMHRKIVVIDARIAFIGGLNYSAEHMSSYGPEAKQDYAVRLEGPIVEDIL
                     QFELENLPGQSAARRWWRRHHKAEENRQPGEAQVLLVWRDNEEHRDDIERHYLKMLTQ
                     AQREVIIANAYFFPGYRFLHALRKAARRGVRIKLIIQGEPDMPIVRVGARLLYNYLVK
                     GGVQVFEYRRRPLHGKVALMDDHWATVGSSNLDPLSLSLNLEANVIIHDRHFNQTLRD
                     NLNGIIAADCQQVDETMLPKRTWWNLTKSVLAFHFLRHFPALVGWLPAHTPRLTQVDP
                     PAQPTMETQDRVETENTGVNP"
     gene            complement(16881..17642)
                     /locus_tag="OO357_001904"
     CDS             complement(16881..17642)
                     /locus_tag="OO357_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5886865.1"
                     /translation="MPDQTQQFSFKVLTINIHKGFTAFNRRFILPELRDAVRTVSADI
                     VCLQEVMGAHEVHPLHVENWPDTSHYEFLADTMWSDFAYGRNAVYPEGHHGNAVLSRY
                     PIEHYENRDVSVDGAEKRGVLYCRIVPPMTGKAIHVMCVHLGLREAHRQAQLAMLAEW
                     VNELPDGEPVLVAGDFNDWRQKANHPLKVQAGLDEIFTRAHGRPARTFPVQFPLLRLD
                     RIYVKNASASAPTALPLRTWRHLSDHAPLSAEIHL"
     gene            17775..18185
                     /locus_tag="OO357_001905"
     CDS             17775..18185
                     /locus_tag="OO357_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhQ family protein"
                     /protein_id="HCR5886866.1"
                     /translation="MKWQQRVRVATGLSCWQIMLHLLVVALLVVGWMSKTLVHVGVGL
                     CALYCVTVVMMLVFQRHPEQRWREVADVLEELTTTWYFGAALIVLWLLSRVLENNFLL
                     AIAGLAILAGPAVVSLLAKDKKLHHLTSKHRVRR"
     gene            complement(18147..19253)
                     /locus_tag="OO357_001906"
     CDS             complement(18147..19253)
                     /locus_tag="OO357_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5886867.1"
                     /translation="MFHRLWTLIRKELQSLLREPQTRAILILPVLIQVILFPFAATLE
                     VTNATIAIYDEDNGEHSVELTQRFARASAFTHVLLLKSPQEIRPTIDTQKALLLVRFP
                     ADFSRKLDTFQTAPLQLILDGRNSNSAQIAANYLQQIVKNYQQELLEGKPKPNNSELV
                     VRNWYNPNLDYKWFVVPSLIAMITTIGVMIVTSLSVAREREQGTLDQLLVSPLTTWQI
                     FIGKAVPALIVATFQATIVLAIGIWAYQIPFAGSLALFYFTMVIYGLSLVGFGLLISS
                     LCSTQQQAFIGVFVFMMPAILLSGYVSPVENMPVWLQNLTWINPIRHFTDITKQIYLK
                     DASLDIVWNSLWPLLVITATTGSAAYAMFRRKVM"
     gene            complement(19264..20397)
                     /locus_tag="OO357_001907"
     CDS             complement(19264..20397)
                     /locus_tag="OO357_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5886868.1"
                     /translation="MSNPILSWHRVRALCVKETRQIVRDPSSWLIAVVIPLLLLFIFG
                     YGINLDSSKLRVGILLEQRSEAALDFTHTMTGSPYIDATISDNRQELIAKMQSGKIRG
                     LVVIPVDFAEQMERANATAPIQVITDGSEPNTANFVQGYVEGIWQIWQMQRAEDNGQT
                     FEPLIDVQTRYWFNPAAISQHFIIPGAVTIIMTVIGAILTSLVVAREWERGTMEALLS
                     TEITRTELLLCKLIPYYFLGMLAMLLCMLVSVFILGVPYRGSLLILFFISSLFLLSTL
                     GMGLLISTITRNQFNAAQVALNAAFLPSIMLSGFIFQIDSMPAVIRAVTYIIPARYFV
                     STLQSLFLAGNIPVVLVVNVLFLIASAVMFIGLTWLKTKRRLD"
     gene            complement(20390..22126)
                     /locus_tag="OO357_001908"
     CDS             complement(20390..22126)
                     /locus_tag="OO357_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5886869.1"
                     /translation="MNDAVITLNGLEKRFPGMDKPAVAPLDCTIHAGYVTGLVGPDGA
                     GKTTLMRMLAGLLKPDSGSATVIGFDPIKNDGALHAVLGYMPQKFGLYEDLTVMENLN
                     LYADLRSVTGEARKQTFARLLEFTSLGPFTGRLAGKLSGGMKQKLGLACTLVGEPKVL
                     LLDEPGVGVDPISRRELWQMVHELAGEGMLILWSTSYLDEAEQCRDVLLMNEGELLYQ
                     GEPKALTQTMAGRSFLMTSPHEGNRKLLQRALKLPQVSDGMIQGKSVRLILKKEATPD
                     DIRHADGMPEININETTPRFEDAFIDLLGGAGTSESPLGAILHTVEGTPGETVIEAKE
                     LTKKFGDFAATDHVNFAVKRGEIFGLLGPNGAGKSTTFKMMCGLLVPTSGQALVLGMD
                     LKESSGKARQHLGYMAQKFSLYGNLTVEQNLRFFSGVYGLRGRAQNEKISRMSEAFGL
                     KSIASHATDELPLGFKQRLALACSLMHEPDILFLDEPTSGVDPLTRREFWLHINSMVE
                     KGVTVMVTTHFMDEAEYCDRIGLVYRGKLIASGTPDDLKAQSANDEQPDPTMEQAFIQ
                     LIHDWDKEHSNE"
     gene            complement(22119..23114)
                     /gene="hlyD"
                     /locus_tag="OO357_001909"
     CDS             complement(22119..23114)
                     /gene="hlyD"
                     /locus_tag="OO357_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="secretion protein HlyD"
                     /protein_id="HCR5886870.1"
                     /translation="MKKPVVIGLAVVVLAAVVAGGYWWYQSRQDNGLTLYGNVDIRTV
                     NLSFRVGGRVESLAVDEGDAIKAGQVLGELDHKPYEIALMQAKAGVSVAQAQYDLMLA
                     GYRDEEIAQAAAAVKQAQAAYDYAQNFYNRQQGLWKSRTISANDLENARSSRDQAQAT
                     LKSAQDKLRQYRSGNREQDIAQAKASLEQAQAQLAQAELNLQDSTLIAPSDGTLLTRA
                     VEPGTVLNEGGTVFTVSLTRPVWVRAYVDERNLDQAQPGRKVLLYTDGRPDKPYHGQI
                     GFVSPTAEFTPKTVETPDLRTDLVYRLRIVVTDADDALRQGMPVTVQFGDEAGHE"
     gene            complement(23117..23788)
                     /gene="cecR"
                     /locus_tag="OO357_001910"
     CDS             complement(23117..23788)
                     /gene="cecR"
                     /locus_tag="OO357_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CecR"
                     /protein_id="HCR5886871.1"
                     /translation="MNNPAMTIKGEQAKKQLIAAALAQFGEYGMNATTREIAAQAGQN
                     IAAITYYFGSKEDLYLACAQWIADFIGEQFRPHAEEAERLFAQPQPDRAAIRELILRA
                     CRNMIKLLTQDDTVNLSKFISREQLSPTAAYHLVHEQVISPLHSHLTRLIAAWTGCDA
                     NDTRMILHTHALIGEILAFRLGKETILLRTGWTAFDEEKTELINQTVTCHIDLILQGL
                     SQRSL"
     gene            24017..25204
                     /gene="rhlE"
                     /locus_tag="OO357_001911"
                     /pseudo
     CDS             24017..25204
                     /gene="rhlE"
                     /locus_tag="OO357_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308902.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase RhlE"
     gene            complement(25411..25893)
                     /gene="ybiA"
                     /locus_tag="OO357_001912"
     CDS             complement(25411..25893)
                     /gene="ybiA"
                     /locus_tag="OO357_001912"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-glycosidase YbiA"
                     /protein_id="HCR5886872.1"
                     /translation="MPVRAQRIQHVMQDTIINFYSTSDDYGDFSNFAARPIKVDGNTW
                     PTSEHYFQAQKFLDEKYREEIRRVSSPMVAARMGRNRSKPLRKNWESVKEQVMRKALR
                     AKFEQHAELRVLLLATAPAKLVEHTENDAYWGDGGNGKGKNRLGYLLMELREQLAIEK
                     "
     gene            26013..28163
                     /gene="dinG"
                     /locus_tag="OO357_001913"
     CDS             26013..28163
                     /gene="dinG"
                     /locus_tag="OO357_001913"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase DinG"
                     /protein_id="HCR5886873.1"
                     /translation="MALTAALKAQIAAWYKALQEQIPDFIPRAPQRQMIADVAKTLAG
                     EEGRHLAIEAPTGVGKTLSYLIPGIAIAREEQKTLVVSTANVALQDQIYSKDLPLLKK
                     IIPDLKFTAAFGRGRYVCPRNLTALASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLK
                     GDLDTYKWDGLRDHTDIAIDDDLWRRLSTDKASCLNRNCYYYRECPFFVTRREIQEAE
                     VVVANHALVMAAMESEAVLPDPKNLLLVLDEGHHLPDVARDALEMSAEITVPWYRLQL
                     DLFTKLVATCMEQFRPKTIPPLAIPERLNAHCEELYELIASLNNILNLYMPAGQEAEH
                     RFAMGELPDELLEICQRLAKLTEMLRGLAELFLNDLSEKTGSHDIVRLHRLILQMNRA
                     LGMFEVQSKLWRLASLAQSSGAPVTKWATREEREGQLHLWFHCVGIRVSDQLERLLWR
                     SIPHIIVTSATLRSLNSFSRLQEMSGLKEKAGDRFVALDSPFNHCEQGKIVIPRMRFE
                     PSIDNEEQHIAEMAAFFREQVESKKYLGMLVLFASGRAMQRFLDYVTDLRLMLLVQGD
                     QPRYRLVELHRKRVANGERSVLVGLQSFAEGLDLKGDLLSQVHIHKIAFPPIDSPVVI
                     TEGEWLKSLNRYPFEVQSLPSASFNLIQQVGRLIRSHGCWGEVVIYDKRLLTKNYGKR
                     LLDALPVFPIEQPEVPEGIVKKKEKTKSPRRRRR"
     gene            28191..29153
                     /gene="ybiB"
                     /locus_tag="OO357_001914"
     CDS             28191..29153
                     /gene="ybiB"
                     /locus_tag="OO357_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein YbiB"
                     /protein_id="HCR5886874.1"
                     /translation="MDYRKIIKEIGRGKNHARDLDRDTARGLYAHMLNGEVPDLELGG
                     VLIALRIKGEGEAEMLGFYEAMQNHTIKLTPPAGKPMPIVIPSYNGARKQANLTPLLA
                     ILLHKLGFPVVVHGVSEDPTRVLTETIFELMGITPTLHGGQAQAKLDEHQPVFMPVGA
                     FCPPLEKQLAMRWRMGVRNSAHTLAKLATPFAEGEALCLSSVSHPEYIGRVAKFFSDI
                     GGWALLMHGTEGEVYANPQRCPQINLIDREGMRVLYEKQDPAGSELLPQAKDPETTAQ
                     WTERCLAGSEAIPESLKIQMACCLVATGEAATISDGLARVNQAF"
     gene            29294..30379
                     /gene="hcxB"
                     /locus_tag="OO357_001915"
     CDS             29294..30379
                     /gene="hcxB"
                     /locus_tag="OO357_001915"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcXB"
                     /protein_id="HCR5886875.1"
                     /translation="MESGHRFDAQTLHSFIQAVFRQMGSEEQEAKLVADHLIAANLAG
                     HDSHGIGMIPSYVRSWSQGHLQINHHAKIVKEAGAAVTLDGDRAFGQVAAHEAMALGI
                     EKAHQHGIAAVALHNSHHIGRIGYWAEQCSAAGFVSIHFVSVVGIPMVAPFHGRDSRF
                     GTNPFCVVFPRKDNFPLLLDYATSAIAFGKTRVAWHKGVPVPPGCLIDVNGVPTTNPA
                     VMQESPLGSLLTFAEHKGYALAAMCEILGGALSGGKTTHQETLQTSPDAILNCMTTII
                     INPELFGAPDCNAQTEAFAEWVKASPHDDDKPILLPGEWEVNTRRERQEQGIPLDAGS
                     WQAICDAARQIGMSEETLQAFCQQLAS"
     gene            complement(30608..30868)
                     /locus_tag="OO357_001916"
     CDS             complement(30608..30868)
                     /locus_tag="OO357_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5886876.1"
                     /translation="MKTINTVVAAMALSTLSFGVFAAEPVTASQAQNMNKIGVVSADG
                     ASTLDALEAKLAEKAAAAGASGYSITSATNNNKLSGTAVIYK"
     gene            complement(31133..31399)
                     /gene="ybiI"
                     /locus_tag="OO357_001917"
     CDS             complement(31133..31399)
                     /gene="ybiI"
                     /locus_tag="OO357_001917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-type zinc finger protein YbiI"
                     /protein_id="HCR5886877.1"
                     /translation="MASAWANDDAVNEQINSTIEDAIARARGEIPRGESLYECEECGA
                     PIPQARREAIPGVRLCIHCQQEKDLQKPAYTGYNRRGSKDRQLR"
     gene            complement(31473..32150)
                     /gene="ybiX"
                     /locus_tag="OO357_001918"
     CDS             complement(31473..32150)
                     /gene="ybiX"
                     /locus_tag="OO357_001918"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415325.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKHD-type hydroxylase YbiX"
                     /protein_id="HCR5886878.1"
                     /translation="MMYHIPGVLSPQDVAHFREQLEQAEWVDGRVTTGAQGAQVKNNQ
                     QVDTRSALYAALQNEVLNAVNQHALFFAAALPRTLSTPLFNRYQNNETYGFHVDGAVR
                     SHPQNGWMRTDLSATLFLSDPQSYDGGELVVNDTFGQHRVKLPAGDLVLYPSSSLHCV
                     TPVTRGVRVASFMWIQSMIRDDKKRAMLFELDKNIQSLKSRYGENEEILSLLNLYHNL
                     LREWSEI"
     gene            complement(32192..33913)
                     /locus_tag="OO357_001919"
                     /pseudo
     CDS             complement(32192..33913)
                     /locus_tag="OO357_001919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415326.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="catecholate siderophore receptor Fiu"
BASE COUNT         8015 a         8763 c         9145 g         7990 t
ORIGIN      
        1 attgatcctc attgtttata tattaatata ttcgtgcaaa tggagtgagg attctttaaa
       61 aaaacgcgcc tgtaaatagt taagaacagg aagaactgtt ggggatgtgg ctggccgaaa
      121 atgaaggggt gaagttctgg ctcaatgtgc tgactgaact gaaaaaacgc ggttggtcgc
      181 cctacctttt catcaagcca gtagtctgcc caccacagca tcatttctct gcgcttatcg
      241 agatactgag catggttgta aattcagcta acccagtgcg ttgacaaaaa attagcgcaa
      301 gaggacaaaa aatcaccttg cgctaatgct ctgtatcagg tcactaatac catctaagtg
      361 gttgattcat agtgactgga tatgttgtgt tttgcagtat tatgcagtct attttttaag
      421 ctaaatgtat tataatgcat tgatattaat gatttttact gtttctcggt cagctttttt
      481 atactaactt gagcgaaacg ggaaggtaaa aagacaaaaa gttgttttta atacctttaa
      541 gtgataccag atggcattgc gccatctggc agagtgatta actaaacatc gcggtaatcg
      601 atgcgcttgc cagagagtgg aaatgaacgt taaacccgac catcgcgccg ctggctcctt
      661 catcgacatc aatacgctct acatccagcg cgtgaacggt aaaaatgtag cgatgggttt
      721 cgcctttcgg cggcgctgcg ccatcgtatc cggttttacc aaaatcggta cgcgtctgca
      781 aaacgccgtc tggcattgct accagaccag agccaaaccc ttgcggtaat acgcgggtat
      841 cagcgggtaa attaacaact acccagtgcc accagccgga gccggttggc gcatccgggt
      901 cgtagcaggt gacaacaaaa cttttcgttc ccgcaggaac atcatcccac gccagatgcg
      961 gtgaaatatt atcgccatcg taacccatgc cgttaaagac atgacgatgc ggcaacttat
     1021 cgccatcgcg cagatcgtta ctgatgagtt tcatgaaccc tcctttcttg tttgcagaaa
     1081 gtgtagccag aaaccctcac gctgacttcc cgttattggc aaaaaaatgt ttcatcctgt
     1141 accgcgcggt taaccgctgc ggtcagacgc tgcaactgtt gcgggagaat aatatagggc
     1201 ggcatcaggt aaatcagttt gccaaaaggc cggatccata ccccctgttc gacaaagaat
     1261 ttttgcagcg ccgccatatt caccggacga gtggtttcga ccacaccgat tgcccccagt
     1321 acgcgcacat cggcaaccat ttcggcatca cgggctggtg ccagttgctc gcgcagctgc
     1381 acttcaatag ccgccacctg ctgctgccat tcgccggatt cgataatcgc taggctggcg
     1441 tttgctgccg cgcaggccag cggattgccc ataaaagttg gcccatgcat aaagcagccg
     1501 gcttcgccgt tactgatggt ttctgcaacc tcgcgcgtgg taagtgtggc ggaaagggtc
     1561 attgtgccgc cggttaaggc tttaccgagg cacaaaatgt ccggcgcgat ttctgcatat
     1621 tcacaggcaa acagtttgcc ggtacgacca aatccggtgg cgatctcgtc ggcaatcagc
     1681 aagatacctt cgcgatcgca cattttgcgg attcgtttta accattccgg atggtacatg
     1741 cgcatcccgc ctgcgccctg gacaatcggc tcaatgatca ccgctgcgat ttcatgacga
     1801 tgcgccgcca tcaggcgggc aaaaccaacc atatcgcgtt catcccattc accatccatg
     1861 cggctttgcg gggcgggagc aaacaagttt tctggcagat agcctttcca caggctgtgc
     1921 attgagttgt ccggatcgca caccgacatc gcgccaaagg tatcgccatg ataaccattg
     1981 cggaaggtca gaaaacgctg gcgcgcttcg cctttggctt gccagtactg caacgccatt
     2041 ttcatcgcca cttccaccgc tacggaaccg gagtccgcga gaaaaacgca ctccagcggt
     2101 tgcggcgtca ttgccaccag tttgcggcat agctcgatgg cgggcgcatg ggtgataccg
     2161 ccaaacatca catgcgacat ggcatcaatt tgcgtcttca tcgccgcatt aagctgcggg
     2221 tgattgtagc cgtggatcgc cgcccaccag gacgacatac cgtcaaccag gcgtctgccg
     2281 tcagacaaaa ttagctcgca accttcggcg ctcgccaccg gataaaccgg cagaggggag
     2341 gtcatggatg tgtatgggtg ccagatatgg cgttggtcaa aggcaagatc gtccgttgtc
     2401 ataatcgact tgtaaaccaa attgaaaaga tttaggttta caagcctaca ccgaattaac
     2461 aacaaaaaac acgttttgga gaagccccat ggctcaccgc ccacgctgga cattgtcgca
     2521 agtcacagaa ttatttgaaa aaccgttgct ggatctgctg tttgaagcgc agcaggtgca
     2581 tcgccagcat ttcgaacctc gtcaggtgca ggtcagcacg ttgctgtcga ttaagaccgg
     2641 agcttgtccg gaagattgca aatactgccc gcaaagctcg cgctacaaaa ccgggctgga
     2701 agccgagcgg ttgatggaag ttgaacaggt gctggagtcg gcgcgcaaag cgaaagcggc
     2761 aggatcgacg cgcttctgta tgggcgcggc atggaagaat ccccacgaac gcgatatgcc
     2821 gtacctggaa caaatggtgc agggggtaaa agcgatgggg ctggaggcgt gtatgacgct
     2881 gggcacgttg agtgaatctc aggcgcagcg cctcgcgaac gccgggctgg attactacaa
     2941 ccacaacctg gacacctcgc cggagtttta cggcaatatc atcaccacac gcacttatca
     3001 ggaacgcctc gatacgctgg aaaaagtgcg cgatgccggg atcaaagtct gctcaggtgg
     3061 cattgtgggc ttaggcgaaa cggtaaaaga tcgcgccgga ttattgctgc aactggcaaa
     3121 cctgccgacg ccgccggaaa gcgtaccaat caacatgctg gtgaaggtga aaggcacgcc
     3181 gcttgccgat aacgatgacg ttgatgcctt tgattttatt cgcaccattg ccgtagcgcg
     3241 gatcatgatg ccgacctctt acgtgcgcct ttctgccgga cgcgagcaga tgaacgaaca
     3301 gactcaggcg atgtgcttta tggcaggcgc aaactcgatt ttctacggtt gcaaactgct
     3361 gaccacgccg aatccggaag aagataaaga cctgcaactg ttccgcaaac tggggctaaa
     3421 tccgcagcaa actgccgtgc tggcagggga taacgaacaa cagcaacgtc ttggacaggc
     3481 gctgatgacc ccggacaccg acgaatatta caacgcggca gcattatgag ctggcaggag
     3541 aaaatcaacg cggcgctcga tgcgcggcgt gctgccgatg ccctgcgtcg ccgttatccg
     3601 gtggcgcaag gagccggacg ctggctggtg gcggatgatc gccagtatct gaacttttcc
     3661 agtaacgatt atttaggttt aagccatcat ccgcaaatta tccgtgcctg gaagcagggg
     3721 gcggagcaat ttggcatcgg tagcggcggc tccggtcacg tcagcggtta tagcgtggtg
     3781 catcaggcgc tggaagaaga actggccgag tggcttggct attcgcgggc gctgctgttt
     3841 atctctggtt ttgccgcgaa tcaggcagtc attgccgcga tgatggcgaa agaggaccgt
     3901 attgttgccg accggcttag ccatgcctca ttgctggagg ctgccagttt aagcccgtcg
     3961 cagcttcgcc gttttactca taacgatgtc gctcatctgg cgcgactgct tgcttccccc
     4021 tgtccggggc agcaactggt ggtgacagaa ggcgtgttca gcatggacgg cgatagcgcg
     4081 ccactggcgg aaatccagca ggtaacgcaa cagcacaatg gctggttgat ggtcgatgat
     4141 gcccacggca cgggcgttat cggggagcag gggcgcggca gctgctggct gcaaaaggta
     4201 aaaccagaat tgctggtagt gacttttggc aaaggatttg gcgtcagtgg ggctgcggtg
     4261 ctttgctcaa gtacggtggc ggattatctg ctgcaattcg cccgccacct tatctacagc
     4321 accagtatgc cgcccgctca ggcgcaggca ttacgtgcgt cgctggcggt cattcgccgt
     4381 gatgaggggg atgcacggcg cgaaaaactg gtgtcactca ttgcgcgttt tcgtgccgga
     4441 gtacaggatt tgccgtttac gctggctgat tcatgcagcg ccatccagcc attgatcgtc
     4501 ggtgataaca gccgtgcgtt acaactggca gaaaaactgc gccagcaagg ctgctgggtc
     4561 acggcgattc gcccgccaac cgtacccgct ggtactgcgc gactgcgctt aacgctaacc
     4621 gctgcgcatg aaatgcagga tatcgaccgt ctgctggagg tgctgcatgg caacggttaa
     4681 taaacaagcc attgcagcgg catttggtcg ggcagccgca cactatgagc aacatgcaga
     4741 tctacagcgc cagagtgctg acgccttact ggcaatgctt ccacagcgta aatacaccca
     4801 cgtactggac gcgggttgtg gacctggctg gatgagccgc cactggcggg aacgtcacgc
     4861 gcaggtgacg gcgttagatc tctcgccgcc aatgcttgcg caagcccgcc ataaagatgc
     4921 cgcagatcat tatctggcag gagatatcga atccctgccg ttagcgactg cgacgttcga
     4981 tcttgcatgg agcaatctcg cggtgcagtg gtgcggtaat ttatccacgg cactccgcga
     5041 gctgtatcgg gtggtgcgcc ccggaggcgt ggtcgcgttt accacgctgg tgcagggatc
     5101 gttacccgaa ctgcatcagg cgcggcaggc ggtggacgag cgtccgcatg ctaatcgctt
     5161 tttaccgcca gatgaaatcg aagagtcgct gaacggcgtg cgttatcaac atcatattca
     5221 gctcatcaca ctatggtttg atgatgcgct cagtgccatg cgttcgctga aaggcatcgg
     5281 tgctacgcat cttcatgaag ggcgcgaccc gcgaatatta acgcgttcgc agttgcagcg
     5341 attgcaactg gcctggccgc aacaacaggg gcgatatcct ctgacgtatc atcttttttt
     5401 gggagtgatt gctcgtgagt aaacgttatt ttgtcaccgg aacggatacc gaagtgggga
     5461 aaaccgtcgc cagttgtgca cttttacaag ccgcgaaggc agtaggttat cggacggcag
     5521 gttataaacc ggtcgcctct ggcagcgaaa agaccccgga aggcttacgt aatagtgacg
     5581 cgctggcgtt acagcacaac agcagcctgc agctggatta cgcaacggta aatccttaca
     5641 ccttcgcaga atccacctcg ccgcacatca tcagcgccca agagggcagg ccgatagaat
     5701 cattggtaat gagcgccgga ttacgcgcgc ttgaacaaca ggctgactgg gtgttagtgg
     5761 aaggtgctgg cggctggttt acgccgcttt ctgacacttt cacttttgca gattgggtaa
     5821 cacaggaaca actgccggtg atactggtag ttggtgtgaa actcggctgt attaatcacg
     5881 cgatgttgac tgcgcaggca atacaacacg ccggactgac tctggcgggt tgggtggcga
     5941 acgatgttac gcctccggga aaacgtcacg ctgaatatat gaccacgctc acccgcatga
     6001 ttcccgcgcc gctgctggga gagatcccct ggcttgcaga aaatccagaa aatgcggcaa
     6061 ccggaaagta cataaacctt gccttgttgt agccattctg tatttggtta aattgcgagc
     6121 gagatcgcgt cttcgattga ctgcaattta accaattaaa ttctaaaata atcacgaaaa
     6181 aaattttact tccgcctcat gcggcgaatg tgggaattgc ccaggcggcg ggggataggg
     6241 gctggagaca gttatccact attcctgtgg ataaccatgt gtattagagt tagaaaacac
     6301 gaggcaagcg agagaatacg cggcttgcac gcgaattggc gttaaagacg gctcaaagaa
     6361 atatctttta ttttttaact ggttagataa atgcaatggc agtcactgaa caggcatctc
     6421 ttgccataaa actgtcatca ctcatcttga caaatgttaa aaaagccgtt gctttgggga
     6481 taacccggta aggctggagt tttatctcgc cacagagtaa attttgctca tgattgacag
     6541 cggagtttac gctgtatcag aaatattatg gtgatgaact gtttttttat ccagtataat
     6601 ttgttggcat aattaagtac gacgagtaaa attacatacc tgcccgccca actccttcag
     6661 gtagcgactc atgagtaaac cgttcaaact gaattccgct tttaaacctt ctggcgatca
     6721 gccagaggcg attcgacgtc tcgaagaggg gctggaagat ggcctggcgc accagacgtt
     6781 acttggcgtg actggctccg ggaaaacctt caccattgcc aatgtcattg ctgaccttca
     6841 gcgcccaacc atggtacttg cgcccaacaa aacgctggcg gcccagctgt atggcgaaat
     6901 gaaagagttc ttcccggaaa acgcggtgga gtatttcgtc tcctactacg actactatca
     6961 gccggaagcc tatgtaccga gttccgacac cttcattgag aaagatgcct cggttaacga
     7021 acatattgag cagatgcgtt tgtccgccac caaagcgatg ctggagcggc gtgatgtgat
     7081 tgtagtggcg tctgtttccg cgatttatgg tctgggcgat cctgatttat atctcaagat
     7141 gatgctccat ctcacggtcg gtatgattat cgatcagcgc gcgattctgc gccgactggc
     7201 ggagctgcaa tacgctcgta atgatcaagc attccagcgt ggtactttcc gcgttcgtgg
     7261 cgaggtgata gatatcttcc cggcagaatc ggatgacatt gcacttcgcg tggaactgtt
     7321 tgacgaggaa gtggaacgat tgtcgttatt tgacccgctg accgggcaga ttgtttccac
     7381 tattccacgt tttaccatct acccgaaaac gcactacgtc acaccgcgcg agcgcatcgt
     7441 acaggcgatg gaggagatca aagaagaact ggccgccaga cgcaaagtgc tgttggaaaa
     7501 caacaaactg ctggaagagc agcggctgac ccagcgtacc cagtttgatc tggagatgat
     7561 ggacgagctg ggctactgtt cggggattga aaactactcg cgcttcctct ccggtcgtgg
     7621 accgggtgag ccaccgccga cgctgtttga ttacctgcct gccgatgggc tgctggtcgt
     7681 cgatgaatct cacgtcacca ttccacaaat tggcggcatg tatcgcggtg accgggcgcg
     7741 taaagagaca ctggtggagt acggcttccg cctgccatca gcgctggata accgtccgct
     7801 taagtttgaa gagttcgaag cattagcgcc gcaaaccatc tatgtttcgg cgacgccggg
     7861 taattacgag ctggaaaaat ccggcggcga tgtggtggat caggtggtgc gtccaaccgg
     7921 attgcttgac ccgattatcg aagtgcggcc ggtggcgaca caggttgatg atcttctttc
     7981 ggagattcgt cagcgagcgg caattaacga acgcgtgctg gtcaccacac tgaccaagcg
     8041 aatggcggaa gatcttaccg aatatctcga agaacacggt gagcgcgtgc gttatcttca
     8101 ctcagatatc gacaccgtcg agcgtatgga gattatccgc gacttgcgtc tgggtgagtt
     8161 cgacgtattg gtagggatca acttactgcg tgaaggtctg gatatgccgg aagtgtcgct
     8221 ggtggcgatc ctcgacgctg acaaagaagg cttcctgcgt tccgaacgtt cgttgatcca
     8281 gaccattggt cgtgcggcac gtaacgttaa cggtaaagcg attctctacg gcgataggag
     8341 cactccatca atggcgaaag cgattggcga aaccgaacgt cgccgtgaga aacagcagaa
     8401 gtacaacgag gaacacggca ttacgccgca aggcttgaac aagaaagtgg tcgatatcct
     8461 ggcgctgggg cagaacattg ccaaaaccaa agcgaagggc agaggaaaat cgcgcccgat
     8521 tgttgagccg gataatgtgc cgatggatat gttgccgaaa gcgttgcagc agaaaatcca
     8581 tgaactggaa gggctgatga tgcaacacgc gcagaatctg gagttcgaag aagcggcaca
     8641 aattcgtgac cagttgcatc agttgcgtga gttgtttatt gccgcatcgt aacaggatag
     8701 cgaagaagac tgatgacaaa cggaaaacag tctgatgcgc tacgcttatc aggcctacat
     8761 tttctccgca atatattgaa tttgcgcggt ttgtaggccg gataaggcgt tcacgccgca
     8821 tccggcataa acaacgcgca ctttgtcttc aataggaagc cggaattttc cttcggattt
     8881 ccgttaacct aaagcctgta acgccttttc cagcgcgtta tgtaacaact ggcggtcatg
     8941 acgatacgga atatcgctgg cctccagtac ctcctggatc acaatccgct ctttcaccgc
     9001 cgagacatcc acttttggcc cgacgatgac cgcatcaatg acctttttac caacatactg
     9061 ctccataatt gccagcttgc tttccagctt caaattagcc gcaggtaaac tcaactcacg
     9121 ccccagattg ccgatataaa ccatcggcgc tggcgtgcgg cgtaatgcct gggcgatttc
     9181 cttcagcagc agaattggca tcaggctggt ataaaaactg ccaggcccaa taatgatgag
     9241 atccgcttca ttgatagcgt gaaccgcctc acgcgttgcg ggtacattag gcgttaacaa
     9301 taactcttga atcggcgtag ttaactggtc gatattgacc tcgccgtaaa cttcatgccc
     9361 ctgatcgtca atcgccatca gatcaacagg atgctctgac attggaatca aatgcgtatc
     9421 cactttcagc agattacgaa ttaaattgat ggcttccaga ggccgcacgc taaggtgatc
     9481 cagcgccttt aacatcaagt ttccgagatt atgaccggaa agttcgccat tgccgccaaa
     9541 acggtattca aacatcgcgg aggcgacgtt cggttccgtt atcagctggt tgaggcagtt
     9601 gcgcatatcg ccccaagcaa tgccgccttc tgaacggcga atacgccccg tcgagccacc
     9661 attatccgtg gtggtgacga tacccgttaa acgagaaccc aaagacgaaa gtgatgagag
     9721 aacgcgtccc agtccatgcc ctccgccgag agcaacgaca cgatcaagat cagccagcgt
     9781 acgattgcgc atatagtttc ctggagtcag attatccgcg ctacagtagc gcaaattgcg
     9841 gggaaacagc aattaacctg ccaatattga ggatacaaac tattttcttc tatctctaaa
     9901 ggacgatgca cgctatgcct ccctgatgat gtatatcaaa gtaaaaccgc cattttccct
     9961 tattctgtag cgaaatagca cgatcatgac gctatataca tgattacata gcgaaagtgt
    10021 ggatggtaaa aatctcattt acacgctagt atcggcttaa ccactaaaca ctctagcctc
    10081 tgcacctggg tcaactgata cggtgctttg gccgtgacaa tgctcgtaaa gattgccacc
    10141 agggcgaagg aagaaatgac ttcgcctccc gtatctggaa aggtgtacat ggcttcacaa
    10201 ctgactgatg tatttgcgcg taagttttac tacttgcgcc tgtcgattac cgatgtgtgt
    10261 aactttcgtt gcacctactg cctgccggat ggctacaaac cgagcggcgt caccaataaa
    10321 ggctttctta ccgtcgatga aattcgccgg gttacgcgcg ccttcgccag tctgggcacc
    10381 gaaaaagtgc gcctgacagg aggagagccg tctttacgcc gcgactttac cgatatcatc
    10441 gccgctgtgc gggaaaacga cgctatccgc cagattgcgg tcaccaccaa tggttaccgt
    10501 ctggaacgcg atgtggcgaa ctggcgcgat gcgggactta ctggcattaa cgtcagtgtc
    10561 gacagtttgg acgcccgcca gtttcacgct attaccgggc aggataaatt caaccaggtc
    10621 atggcaggga ttgatgctgc atttgaggcc ggttttgaga aggtcaaagt caataccgtg
    10681 ctgatgcgtg atgttaatca tcaccagctc gacacctttc tgaactggat ccagcatcgc
    10741 cctatccagc tgcgtttcat cgaactgatg gaaacgggcg agggcagcga gctcttccgt
    10801 aagcatcaca tctctggtca ggttctgcgt gacgagctac tgcgtcgcgg ctggatccac
    10861 caattacgtc aacgcagcga cggtcccgcg caagtctttt gccatccaga ttacgccgga
    10921 gagattggcc ttatcatgcc gtatgaaaaa gacttctgcg ccacttgcaa ccgcctgcgc
    10981 gtttcctcca ttggtaaact ccatctctgc ctgtttggtg aaggcggcgt taacctgcgc
    11041 gatctgctgg aaggcgatac ccagcaacag gcgctggaag cgcgtatttc agcggcgctg
    11101 cgggagaaga aacagaccca tttcctgcat caaaacaaca ccggtattac gcaaaactta
    11161 tcgtacattg gcggctaaaa cgtcaaaagg agagatcaga tgagtcaggt aagcactgaa
    11221 tttatcccga cccgtattgc tattcttacg gtttctaatc gtcgcggtga agaagacgat
    11281 acctccggtc actatctgcg cgattcggcg caagaagcgg gccatcacgt tgtcgatgaa
    11341 gccatagtga aagaaaaccg ctacgctatt cgcgctcagg tatctgcgtg gatcgccagc
    11401 gacgatgtac aagtggtatt gattacgggt ggtactggcc tgacggaagg tgatcaggct
    11461 cccgaagcgc tgctgccgtt gttcgaccgt gaggttgaag gttttggtga agtgttccgt
    11521 atgttgtcgt ttgaagagat tggcacttcc acgttgcaat ctcgtgcggt agcgggcgtc
    11581 gctaacaaaa cgctgatttt cgccatgccg ggttcgacca aagcgtgccg taccgcatgg
    11641 gaaaatatca tcgcgccgca gctggatgcc cgtacgcgtc cgtgtaattt ccatccacat
    11701 ttgaagaaat aagtatgtcg caactgaccc atatcaacgc cgctggcgaa gcgcacatgg
    11761 tggatgtctc cgccaaagcg gaaaccgtgc gtgaagcgcg ggcggaagcc tttgtcacca
    11821 tgcgcagcga gacgctggcg atgattattg atggtcgcca ccacaaaggc gacgtatttg
    11881 ccactgcgcg tattgccggt attcaggcgg caaaacgcac ttgggatctg atcccgctgt
    11941 gtcacccgct gatgctcagc aaagttgaag tcaatttaca ggccgagccg gagcacaatc
    12001 gggtgcgtat agaaacctta tgccgcctga ccgggaaaac cggtgtcgaa atggaagcgt
    12061 taaccgcggc ctccgtggcg gcgctgacca tttatgacat gtgcaaagcg gtgcaaaaag
    12121 atatggtgat tggtccggta cgtttgctgg cgaagagcgg cggcaagtcg ggtgacttta
    12181 aggtggaagc ggatgattaa agttcttttt ttcgcccagg tgcgcgagtt ggtgggaaca
    12241 gatgcaaccg aagtggctgc ggattaccca accgttgaag cgttacgcca gcacctggct
    12301 gcgcagagcg atcgctgggc gctggcgctg gaagatggca aattgctggc tgccgtcaac
    12361 cagacgctgg tgagttttga ccatccgctg actgacggcg acgaagtagc tttcttcccg
    12421 ccggtaaccg gaggttaaga tggcagaaac caaaattgtt gtcggcccgc agccgttcag
    12481 cgtaggagaa gagtacccgt ggctggcgga gcgtgacgaa gacggtgcgg tagtcacctt
    12541 caccggaaag gtgcgcaacc ataacctggg cgacagcgtc aaagcattaa ccctcgaaca
    12601 ctatccgggg atgactgaaa aagcactggc agaaattgtt gatgaagcgc gtaaccgctg
    12661 gccgctgggg cgcgtcacgg tgattcaccg catcggggaa ttatggccgg gcgatgaaat
    12721 cgtttttgtc ggtgtcacca gtgcgcatcg cagcagtgcg tttgaagccg ggcagtttat
    12781 tatggattat ctcaaaaccc gcgcaccgtt ctggaagcgc gaagccacgc cggaaggcga
    12841 ccgctgggtt gaagcccggg agagcgatct gcaggcggca aaacgctggt agttttttgt
    12901 tagccggata aggcaccgct gcgtccggca atttcaccgc tcagtacatg cgtttacgct
    12961 gcgtcgatgc acagcctcat cattttgcag tatccttaag atattcctta tatcttcagg
    13021 agatcgtcat ggacagattc ccacgttctg attcaatcgt acaaccctgg gctggcttgc
    13081 aaacttatat ggcgcaagtc tatggctgga tgaccgttgg cttgttgctg accgcatttg
    13141 ttgcctggta tgcggctaat tccgcggccg tgatggagct gttgttcact aaccgtgtct
    13201 ttttaatcgg tctgatcatc gcgcaattag cattggttat tgtgttatca gcgatgattc
    13261 aaaagctgag cgcaggtgta acgacgatgc tctttatgct ttattcggcg ctgacgggtc
    13321 ttacgctttc cagtatattc attgtctata ccgctgcttc tatcgccagt actttcgtcg
    13381 ttactgccgg gatgttcggc gcaatgagcc tgtacggtta caccacgaag cgcgatttaa
    13441 gtggctttgg caatatgctg tttatggcgt taatcggcat tgtgctggca tcgctggtca
    13501 acttctggct gaaaagcgaa gcattgatgt gggcagttac ctacatcggc gtgattgtct
    13561 ttgtcggatt gacggcgtat gacactcaga aattgaaaaa tatgggtgag cagattgata
    13621 cccgcgacac gtcgaacctg cgcaaatatt ccattcttgg tgcgttaacc ttgtatctgg
    13681 acttcatcaa cctgttcctg atgttgttac ggatcttcgg caaccgccgt taattctttt
    13741 tcaccatcac tatcccggcc atgtgtcggg atttttttct tactaaaaac gacacttaac
    13801 aagtaccagg tataaaatat gccttatgag cgagcggaat cgttttccgt atccataaat
    13861 acaaaagtat tttgtatgcg tggttttaaa tttattgatg gtgaatatta atgttttttc
    13921 taaggacaac taaaagaatg gaatcatact cgcaaaacag caataaatta gattttcagc
    13981 acgaggccag gatattaaac ggtatatggc tcattaccgc tttaggtttg gtggcaaccg
    14041 caggactagc ctggggagct aagtatctcg aaattacggc aaccaaatat gattcaccac
    14101 caatgtatgt cgccataggg ttattattac tttgtatgta tggcttaagt aaggatatca
    14161 acaagataaa tgccgccatc gcgggcgtaa tatatctgtt tttactctct ttggtggcga
    14221 ttgtcgttgc aagtttagtt cctgtatatg ccattatcat cgtgttcagc actgcgggcg
    14281 cgatgttttt aatcagtatg ctggccggtt tattatttaa tgttgatcct ggttctcacc
    14341 gttttatcat tatgatgacg ttgacagggt tggccctggt aatcatcgtg aatgcggcat
    14401 taatgagtga acggcccatt tgggtaataa gttgcttaat gattgtgtta tggccaggca
    14461 ttatctcgca tggacgaaat aagctccttg aactggcagg aaaatgccat agtgaagagt
    14521 tgtggagtcc ggttcgttgc gcttttacag gtgcattaac actctattac tattttatcg
    14581 gcttctttgg gatacttgcc gcgatagcta taacgcttgt ctggcaaagg catacgcgtt
    14641 tttttcatta gtcgtaaatg cagatactaa agaacttaac taaacttcac atcgccgctt
    14701 catttttcgc ccgtagcttc ttcgcttggc tttccagtag caggtaacag atcagcgcca
    14761 gcagcagcgg gataaagtaa tacaggacgc gataagcgag cagggcggcg ataatgctcc
    14821 ctttcgaggt atgctcccca gccagtagtg cgataaacac cgcttccagc acaccgatcc
    14881 ccgccggaat atgcacgatg acgccagcaa tactactaac cagtaacacg cccagtacaa
    14941 agaaatagtt cacgctttga ccaagtaaca gccagataat cgcccccatt accatccagt
    15001 taacgctgga aatcagcatc tgagcgaggg cgaatttcca tgaaggcagc accagctttt
    15061 gccctttgat ggtcatatgg cggtgcttcg cgaaagcgca aaaccacaaa taaaccgcga
    15121 taatcatcag taagccaatg ccgagaatac gcagcgtagt ttgatcgaca taccagtaat
    15181 ccggcaactc caccacgcct gcggtaaaga taatccctgc cagtaaaata tagcccagcc
    15241 agttggtggt aatactgagc gaggaaatcc gcgtaatagt gctgcccggt aaccctagtc
    15301 gggagtacaa acgatagcgc ataccaatgc cgccgaccca ggtactgagc gtcaggttga
    15361 aggcgtagca gataaacgac accagcatca cctggcgctt cgccagtttg tgaccgcagt
    15421 aaaagcgggc gagcaggtca tagcagccgt aaatcagata gctgacgacc accagcccga
    15481 ccgcactaag cagcgcaacg cgattgtagt cgcggatgac cttccagacc tcttcccagt
    15541 ccactttttt ggcgtagacc accagcaaca caatcaccgc gataaaaaac agccaggtga
    15601 ggatcttctt tgctaagcgc cagcgcgggt gtgatttact catcaggggt ttacccccgt
    15661 gttttcagtt tctacccgat cctgcgtttc cattgtcggt tgtgcggggg gatcaacctg
    15721 cgtcagacgt ggtgtgtgtg ccggaagcca gccaaccagc gccgggaagt ggcgtaaaaa
    15781 gtggaacgcc agcacgcttt tggtcaggtt ccaccaggtg cgtttgggca gcatggtttc
    15841 atccacctgc tgacaatctg cggcaataat gccgttcaga ttatcgcgca gcgtctggtt
    15901 aaaatgacga tcgtggatga tgacatttgc ttcgagattc agtgacaaac tgagcggatc
    15961 gagattactg gaccctactg tcgcccagtg atcgtccatc aatgccactt tgccgtggag
    16021 cgggcggcgg cggtactcaa aaacctgaac gccgccttta accagatagt tatacagcaa
    16081 gcgcgcaccg actctgacaa tcggcatatc cggttcgccc tgaatgatca gtttgatccg
    16141 caccccgcgc cgtgccgctt tacgcaaggc gtgtaaaaat cgatagccgg ggaagaagta
    16201 ggcgttggcg ataatcactt cccgctgcgc ctgagtgagc attttcagat aatggcgttc
    16261 aatatcatcg cgatgttctt cgttatcgcg ccagaccagc aatacctgcg cttctcccgg
    16321 ctggcggttc tcttccgctt tgtgatgacg tcgccaccag cgtcgtgccg cgctctgtcc
    16381 aggcaggttt tccagctcaa actggagaat atcttcgaca atcggccctt caaggcgtac
    16441 cgcgtaatcc tgtttagcct ctggaccgta gctggacata tgctcggcgg agtaattcag
    16501 cccgccaata aaggcgatac gcgcgtcgat caccacaatt ttgcgatgca tccggcgaaa
    16561 cacattggtg cgcataccaa aaaggcgagg gcggggatcg tagtagcgga acactacgcc
    16621 agctgccgtc agttcattga caaactcatc gctgagatcc ggcgaaccgt agccatccag
    16681 caagacttcc gctttaaccc cgcgttgcgc tgctgccagt agtgccgcat gcagctgttt
    16741 gccgacgtta tcctcaaacc agataaacgt ttcaagaatg atgcgttctt gtgcctcgcc
    16801 aatcgcctta aacaccgcgg gataatattg ctcgccgttt tccagcaact ggatcttatt
    16861 gccttcgcgc cagctacatt tcataaatga atctccgcac ttaaaggggc atgatcagaa
    16921 aggtgtcgcc atgtccgcag cggcaacgcg gttggcgcgc tggcgctggc atttttgacg
    16981 tagatcctgt ccagtcgtag tagaggaaat tgcaccggaa acgtgcgcgc cgggcgtccg
    17041 tgggcgcggg taaaaatctc atccagtccg gcctgcactt ttaacggatg attagctttt
    17101 tgccgccagt cattgaaatc acccgccacc aataccggtt cgccgtccgg tagctcattc
    17161 acccattcgg cgagcatcgc aagctgcgcc tgacggtgcg cctcacgcag gcccagatgt
    17221 acgcacatca catgaatcgc ttttccggtc atcggcggca caatgcggca gtagagcacg
    17281 ccgcgctttt ccgcaccatc gaccgaaaca tcgcgattct cataatgttc aatgggataa
    17341 cgcgacagta cggcgttgcc gtgatgccct tccgggtata cggcattgcg accgtaggca
    17401 aaatcgctcc acatagtgtc ggcgagaaac tcgtagtgcg aggtatcggg ccagttttcc
    17461 acatgcagcg gatgaacttc gtgcgcgccc atcacttcct gcaggcaaac aatatcggcg
    17521 ctgacggtac gcacggcatc gcgaagttcc ggcaaaatga agcgtcggtt aaacgcggta
    17581 aagcctttgt gaatgttgat ggtgagcacc ttgaacgaaa attgttgtgt ttgatcgggc
    17641 ataattttcc tgtctttgcc tcttatctca ttgaaatagt gtagtcggcg tcacaaaaag
    17701 gtgcggtctt acggaatttt ccgtaaagtt cggtactctg agtaagtaga gataaattct
    17761 tcaggagaga agccatgaag tggcaacaac gtgttcgtgt cgcaacgggt ctaagttgct
    17821 ggcagattat gttgcattta ctggtagtgg cgctgctggt ggtgggctgg atgagtaaga
    17881 ctctggttca cgtcggcgtg ggattatgcg cactgtattg tgtcacggta gtgatgatgc
    17941 tggtgtttca gcgccacccc gagcaacgct ggcgtgaggt ggcagacgtg ctggaagagc
    18001 tgaccacgac ctggtatttt ggcgcagcgc tgattgtgct gtggctgttg tcccgcgtac
    18061 tggaaaacaa ctttttgctg gcaattgcag ggctggcgat ccttgctggc ccggcggtag
    18121 tgtctttgct ggcgaaagat aagaagttac atcaccttac gtctaaacat cgcgtacgcc
    18181 gctgaccctg tcgtggccgt tatcaccagt agcggccaca aactattcca cacaatatcc
    18241 agactcgcat ccttcaaata aatctgcttg gtaatgtccg taaagtggcg aatagggtta
    18301 atccacgtca gattttgcag ccacactggc atgttttcca ccggcgaaac gtaaccggaa
    18361 aggagaatgg cgggcatcat aaaaacaaac acgccgataa acgcctgctg ttgtgtcgag
    18421 cagagtgatg aaatcaacag accgaatccc accagcgata aaccgtaaat caccatcgta
    18481 aagtagaaca gcgctagcga tccggcgaag gggatttgat acgcccagat accaatcgcc
    18541 agcacaatgg tggcctggaa ggtggcgaca attaacgccg gtacggcttt gccgatgaag
    18601 atctgccagg tggtgagcgg cgaaaccagt agctgatcga gcgtaccttg ttcacgttcg
    18661 cgggcgacgg aaagtgaagt gacgatcatt acgccgatag tggtgatcat ggcgatcagc
    18721 gacggcacca caaaccattt gtagtcgaga ttcgggttat accagttgcg taccaccagc
    18781 tcgctgttgt tgggtttcgg ttttccttcc agcagctcct gctgataatt tttgacgatc
    18841 tgttgcaggt agttggcggc aatttgcgca ctgttggagt tacgcccgtc gaggatcaac
    18901 tgcaacggcg cggtctggaa ggtatccagt ttgcgcgaga agtcagccgg gaaacgcacc
    18961 agtagtaacg ccttttgtgt gtcgatggtc gggcggatct cctgtgggct tttcagcagc
    19021 agcacatgtg taaaggcgct ggcgcgggca aaacgttgag taagttccac cgaatgctcg
    19081 ccgttatctt catcgtagat ggcgatggtg gcgttagtca cttccagcgt ggcggcgaac
    19141 gggaacagga tcacctgaat tagcacgggt aaaatcagaa tcgcgcgggt ttgtggttcg
    19201 cgcagcaacg actgcaactc tttgcggatt aacgtccata agcgatgaaa catgctcttc
    19261 tccctaatcc agccgacgtt tggttttcag ccacgtcagg ccgataaaca tcaccgccga
    19321 agcgatcaaa aacagcacgt ttaccaccag caccactgga atattcccgg cgaggaacag
    19381 gctttgcagg gtgctgacga aataacgagc gggaataatg tacgtcaccg cgcggatcac
    19441 cgcgggcata ctgtcgatct gaaaaataaa gccggaaagc ataatcgacg gcagaaaagc
    19501 ggcgttcagg gcgacctgag cggcattgaa ctggttgcgg gtaatcgtgg aaatcagcag
    19561 ccccattccc agggtactga gtaaaaacag gctggagata aaaaacagaa tcagcagcga
    19621 accgcgatac ggcacgccga gaataaacac tgacaccagc atacacagca acatcgccag
    19681 catcccgaga aagtaataag ggatcagctt acacagcagc agttccgtgc gggtaatctc
    19741 cgtagagagc agagcctcca tggtgccgcg ttcccattct cgcgccacca ccagcgaggt
    19801 gagaatcgcg ccgatgaccg tcatgataat ggtcaccgca cccgggataa taaagtgctg
    19861 gcttatcgcc gccgggttaa accagtagcg ggtttgtaca tcaataagcg gttcaaaagt
    19921 ctgcccgttg tcctccgctc gctgcatttg ccagatctgc cagatccctt cgacataccc
    19981 ctgcacaaag ttagcggtat tcggctcact gccgtcggtg atcacctgaa tcggtgcggt
    20041 ggcgttggcg cgctccatct gttcggcgaa atccacggga ataaccacca gaccgcgaat
    20101 tttccccgac tgcattttgg cgatcagttc ctgacggtta tcgctgatgg tggcgtcgat
    20161 gtagggcgaa ccggtcatgg tgtgggtgaa atccagcgcc gcttcgctac gctgttccag
    20221 caaaatcccg acccgcagct tgctggagtc gaggttaatg ccgtaaccaa aaataaacag
    20281 cagtagcagt gggatcacta ccgcaatcag ccagctactc ggatcgcgaa cgatctgccg
    20341 cgtctctttg acgcacagcg cccgcacgcg atgccaggaa aggatcgggt tactcattgc
    20401 tatgctcctt atcccagtcg tggatcaact gaataaaggc ttgctccatg gtgggatcgg
    20461 gttgctcatc gttagctgac tgtgctttca aatcgtccgg cgtaccgctg gcgattaatt
    20521 tcccacggta caccaggccg atgcggtcgc aatattccgc ttcatccata aagtgggtgg
    20581 tgaccatcac cgtgacgcct ttctctacca tgctgttgat gtgcagccaa aattcacggc
    20641 gggtgagggg gtcaacgccg gaagtcggtt cgtcgagaaa gagaatatcc ggttcatgca
    20701 tcagcgaaca ggccagcgcc agccgctgtt taaaacctaa tggcagttca tcggtggcgt
    20761 gtgaggcaat acttttcagg ccaaatgcct cgctcatacg ggagattttt tcgttctgcg
    20821 cccgaccgcg taagccatac acaccagaga aaaagcgtaa attctgttcg accgtcaggt
    20881 tgccgtagag cgaaaatttt tgcgccatat agccgagatg ctggcgcgct ttaccggaac
    20941 tctctttcag atccatcccc agcaccagcg cctggccgga agtcggcacc agcaaaccgc
    21001 acatcatctt aaaggtggtc gatttacccg cgccgtttgg ccccagcaaa ccaaaaatct
    21061 ccccacgttt aacggcaaag ttgacgtgat cggtggcggc aaaatcacca aatttcttgg
    21121 tcagttcttt cgcttcgatc accgtctcgc cgggtgtgcc ttctaccgta tgcaatattg
    21181 cacccagcgg cgattctgag gttccggcac cgcccagcaa atcaataaac gcatcttcaa
    21241 aacgcggcgt agtttcgttg atgttgattt ccggcatccc gtcggcatgg cgaatatcgt
    21301 ctggtgtggc ctcttttttg aggatcagac gtaccgattt cccctgaatc atgccatcgc
    21361 tgacctgcgg cagtttcaag gcgcgttgca acagtttgcg gttgccctcg tgtggactgg
    21421 tcatcagaaa gctgcgtccg gccatggttt gtgtcagggc ttttggttct ccctgataca
    21481 gcaactcgcc ttcgttcatc agtaacacgt cacggcactg ctcggcttcg tcgagatacg
    21541 aggtactcca gaggattaac atcccttcgc ccgccagctc atgcaccatc tgccacagtt
    21601 cgcgccgtga gatagggtca acgccgacgc cgggttcatc gagcagcaac actttcggtt
    21661 cgcccaccag ggtacaggcc aggccgagtt tttgtttcat cccaccggag agcttgcccg
    21721 ccaggcgtcc ggtaaacggc ccaagagacg taaactccag caggcgagca aaagtttgct
    21781 tacgtgcctc gccggtgacg ctgcgcaaat ccgcgtacag attgaggttc tccatcaccg
    21841 tgagatcttc atacagacca aatttctgcg gcatatagcc gagcacggcg tgcagcgcgc
    21901 cgtcgttttt gatcggatca aagccaatca ccgtggcact gccgctgtcg ggtttcagta
    21961 atcccgccaa catccgcatc agtgtggttt tacctgcacc gtccggcccc accaaccccg
    22021 tcacataacc ggcgtgaatg gtacaatcga gcggcgcgac ggcgggcttg tccatgcccg
    22081 gaaagcgttt ttccaggccg ttcagcgtga taacggcatc attcatgtcc tgcctcgtca
    22141 ccgaattgta ccgtcactgg cattccctgg cgtaacgcat catcggcgtc ggtcaccaca
    22201 atacgcaggc gatagacgag gtcggtacgc agatccggtg tttcgacggt tttcggggta
    22261 aattcagcag tcggcgaaac gaaaccaatc tgcccgtgat acggcttgtc cgggcgacca
    22321 tcggtataaa gcagcacttt gcgccccggc tgggcctggt caagattccg ttcatcaaca
    22381 taagcgcgca cccacaccgg acgcgttagt gaaacggtaa acaccgtgcc accttcattg
    22441 agaaccgtgc ctggctctac cgcgcgcgtt aacagcgtgc catcagacgg tgctatcaac
    22501 gttgagtcct gtaaattcag ctccgcctgc gccagttgcg cctgcgcctg ttcgaggctg
    22561 gctttcgcct gagcgatgtc ctgttcacgg ttaccggaac ggtactgacg caatttatcc
    22621 tgtgctgatt tcagcgttgc ctgtgcctgg tcgcgcgagg agcgggcatt ttccaggtca
    22681 tttgccgaaa tggtgcggct tttccacagt ccttgctggc ggttatagaa gttctgcgca
    22741 tagtcatagg cggcttgcgc ctgtttcacc gctgcggcgg cctgagcgat ttcttcatcg
    22801 cgatacccgg caagcatcag atcatactgc gcctgtgcca ccgagacgcc cgctttcgcc
    22861 tgcatcaggg caatctcata cggcttgtga tccagttcgc ccagcacctg gcccgctttg
    22921 atagcatcac cttcgtccac cgccagcgat tcaacgcgcc ccccaacacg gaaactaaga
    22981 ttcaccgtac gaatatccac gttgccatac agcgtcaggc cgttatcctg gcggctttga
    23041 taccaccagt agcctccggc aaccacggcg gcaagtacca ctaccgccag tccgatcacg
    23101 acaggttttt tcatcactac aaactccttt gcgataatcc ttgcaaaatc aggtcgatat
    23161 gacaggtcac cgtctggttg atcagttcgg tcttttcttc atcgaacgcg gtccagccgg
    23221 tacgtaacag aattgtttct ttgccaagac ggaacgccag aatctcacca atcaacgcat
    23281 gggtatgaag gatcatgcgg gtgtcattag cgtcgcagcc ggtccaggcg gcaatcaggc
    23341 gtgtcaggtg gctgtgtagc ggactaatca cctgttcgtg caccaggtgg taggctgccg
    23401 tgggagagag ctgctcacgg gagataaact tgctgaggtt gacggtgtca tcctgggtga
    23461 gcagcttaat catgttcctg caggcgcgaa ggatcagttc acggatggca gcccgatcag
    23521 gctgtggttg ggcgaacaag cgttcggctt cctcggcatg cggacggaac tgctcgccaa
    23581 taaaatcggc aatccactgg gcgcaggcga ggtacaaatc ttctttcgaa ccgaagtagt
    23641 aggtgatggc agcgatattc tgcccggcct gggcggctat ctcgcgggtg gtggcgttca
    23701 ttccatattc accaaactgc gccagtgcgg cagcaatcag ctgttttttc gcctgttcac
    23761 ccttgattgt catggcagga ttattcatcg cacagtccat tcttaatcaa atgattgatt
    23821 aagattatga ctccatatgg gagttgtcca gtatggctaa gaattttagc tacgccagtc
    23881 acagggataa tttatgcgct gcgtcacaaa aactgctaca ctccgctccc tcatgacatt
    23941 gtggtttttg tcattttcct tttcagtatc tccctgaaaa ctacaccggt aacggtcggg
    24001 gcggttcgga gtagttatgt ctttcgattc tttgggttta agccctgata tcctgcgcgc
    24061 cgttgccgag cagggttacc gtgaacccac ccctattcag cagcaggcga tccctgctgt
    24121 gctggaaggc cgcgacctga tggctagcgc ccagaccggc accggcaaaa cagcgggctt
    24181 tacgctgccg ctgttgcaac acctgatcac tcgccagccg cacgccaaag ggcgtcgtcc
    24241 ggtacgtgcg ctcattctta ccccgacccg tgaactggcg gcacagattg gcgaaaacgt
    24301 ccgtgattac agcaaatacc tgaacattcg ttcgctggtg gtgtttggcg gtgtcagcat
    24361 taacccacaa atgatgaagt tgcgcggcgg cgttgatgtg ctggtggcaa cgccgggacg
    24421 tttgctggac ctggaacatc aaaatgcagt gaagctggat caggttgaaa tcctcgtcct
    24481 cgatgaagct gaccgcatgc tcgacatggg ctttatccac gatatccgtc gcgtgttaac
    24541 aaaactgcca gcgaaacgcc agaacctgct gttctctgcg accttctctg acgatattaa
    24601 agccctggcg gaaaaactgt tgcacaaccc gctggaaatc gaagtggcac gccgcaatac
    24661 cgcgtctgat caggtgaccc agcacgttca ctttgtcgat aagaaacgca aacgcgaatt
    24721 gctgtcgcac atgattggta aagggaactg gcagcaggtg ctggtgttta cccgtaccaa
    24781 acacggcgct aaccatctgg ctgagcagct caataaagat ggcatccgta gtgtggcgat
    24841 ccacgggaat aaatcgcaag gtgcgcgtac ccgtgcgctg gctgatttta aatcgggtga
    24901 cattcgcgta ctggtggcga ccgatatcgc tgcgcgcggt ctggatatcg aagagttgcc
    24961 gcacgtcgtc aactatgaac tgccaaacgt accggaagat tatgtccacc gtatcgggcg
    25021 taccggtcgt gcggctgcta ccggtgaagc gttgtcgctg gtgtgtgttg atgaacacaa
    25081 actgctgcgt gatatcgaaa aactgctgaa aaaagagatc ccgcgcattg cgattccggg
    25141 ctatgagccg gacccgtcaa tcaaagccga accgatccag aacggtcgcc agcaacgtgg
    25201 cggcatcagg ctgatgaaca aacgcaaaac tgcctgatgc gctacgctta tcaggcctac
    25261 gtgaactctg caatatattg aatttgcatg cttttgtagg ccggataagg cgtttacgcc
    25321 gcatccggca tttcacaaca agcacttgtc agcaatttga gaacacggga aaaaatttat
    25381 ctgttttacc gcccataagc caccgagacg ttacttctct atagccaatt gttcgcgcaa
    25441 ctccattaaa aggtagccca gtcgattctt gcccttacca ttaccaccgt ctccccagta
    25501 agcatcgttt tccgtatgct caaccagttt tgcgggcgcg gttgccagca agagcacacg
    25561 cagttctgca tgctgttcga atttagcgcg aagtgcttta cgcatcacct gttctttaac
    25621 cgactcccag tttttacgaa gtggcttgga acgattacgc cccatgcgag cggcgaccat
    25681 gggggaagaa acccgacgga tctcttcgcg gtatttttca tcaaggaatt tttgtgcctg
    25741 aaaatagtgt tcggaggtgg gccaggtatt cccgtcaacc ttgatgggcc tggcggcaaa
    25801 gttagaaaaa tcaccgtagt catcgctggt gctgtagaaa tttatgatgg tgtcttgcat
    25861 gacgtgttgg atcctttgtg ctcgaacggg cattaaaccg cattatgttg gtggttattg
    25921 cgagccgctt tccagaaaca gaaaaaccat tacccctgaa aaccgaaaaa tgccacaata
    25981 ttggctgttt atacagtatt tcaggttttc tcatggcatt aaccgccgcg cttaaagcgc
    26041 aaattgccgc ctggtataag gcgcttcagg aacagatccc cgactttatt ccccgtgcgc
    26101 cgcagcggca gatgattgcg gacgtcgcca aaacgctggc cggagaagaa gggcggcatc
    26161 tggcgattga agcccccacc ggcgttggga aaacgctctc ctatttgatc cccggcatcg
    26221 ccattgcccg cgaagagcaa aaaacgctgg tggtgagtac cgccaacgtg gcgttgcagg
    26281 atcagattta cagcaaagat ttaccgctgc tgaaaaagat cattcccgat cttaaattca
    26341 ctgccgcttt cgggcgtggg cgctacgttt gtccacgtaa tctgacggcg ctcgccagca
    26401 ctgaacccac gcaacaggat ctgctggcgt ttcttgacga cgaactgacg ccaaataatc
    26461 aggaagagca aaaacgttgt gcgaagctga agggcgatct cgacacttat aaatgggatg
    26521 gtctgcgcga tcatacggat atcgctatag atgatgatct ctggcgtcgt ttgagtaccg
    26581 acaaagccag ctgcctcaac cgtaactgtt actactatcg tgaatgcccg ttttttgtta
    26641 ctcgtcggga gattcaggaa gcggaagtgg tggtggcaaa ccatgcgctg gtgatggcgg
    26701 cgatggaaag cgaagccgta ctgcctgacc cgaaaaatct gctgctggtg ctggacgaag
    26761 gtcatcacct gccagatgtg gcccgggatg cgctggagat gagcgctgaa atcaccgtgc
    26821 cctggtatcg actacagctg gacttgttca cgaaactggt cgctacctgc atggagcagt
    26881 ttcgcccgaa gaccatccca ccgctggcga tccctgaacg tttgaatgcg cattgtgaag
    26941 agttgtatga gcttatcgcc tcgttaaaca acattctcaa tctctacatg cctgccgggc
    27001 aggaggcaga gcaccgtttt gcgatgggcg aactgcctga tgaattgctg gagatctgcc
    27061 agcggctggc aaaactcacc gagatgctgc gtggtctggc ggagttattt cttaacgatt
    27121 taagtgagaa aaccggcagc cacgatattg tgcgtctgca tcggttgatt ttacagatga
    27181 accgcgcgct ggggatgttt gaggtgcaaa gcaaactctg gcggctggcg tctctggcgc
    27241 aatcttccgg tgcgccggtg accaaatggg cgacgcggga agagcgcgaa gggcagctac
    27301 acctctggtt tcactgcgtg ggaatacgtg tcagcgatca gctggaaagg ctgctgtggc
    27361 gcagtattcc gcacattatt gtcacctccg caaccttgcg ttcgctgaat agtttttcgc
    27421 gtttgcagga gatgagtggt ctgaaagaga aagcgggcga ccgttttgtg gcgctggatt
    27481 ccccctttaa ccactgcgaa cagggcaaaa ttgttattcc ccggatgcgc tttgagcctt
    27541 ccatcgacaa cgaagagcaa cacattgccg aaatggcggc ctttttccgt gaacaggtgg
    27601 agagcaaaaa atatctcggt atgttggtac tgtttgccag cggacgggcg atgcagcgct
    27661 ttctcgacta tgtgacggat ttacgcctga tgttgctggt gcagggcgat cagccgcgtt
    27721 atcgcttagt tgaactgcac cgcaaacgcg ttgccaacgg tgagcgtagc gtgctggtgg
    27781 gcttacagtc atttgccgaa gggcttgatt tgaaaggtga tctgctcagc caggtgcata
    27841 tccacaaaat cgcttttccg cccatcgaca gcccggtggt gatcaccgaa ggggaatggc
    27901 tgaaaagcct caaccgctat ccgtttgagg tgcaaagcct gccgagcgcc tcgtttaacc
    27961 tgattcagca ggttgggcga ctgattcgaa gccacggttg ctggggcgaa gtggttatct
    28021 acgacaaacg cttgctgaca aaaaattatg gcaagcgact actggatgca ttaccggtat
    28081 ttccgataga gcaaccggaa gtccctgaag gtatagttaa aaagaaagaa aaaacgaaat
    28141 ccccacgccg tcggcggcgt taatgatgtg agtcaggtaa ggagtcgtaa atggactatc
    28201 gcaaaatcat taaagagatc gggcgcggga aaaaccacgc gcgcgattta gaccgggata
    28261 ctgcgcgcgg tctgtatgct catatgctca atggtgaagt ccctgacctt gagttgggcg
    28321 gcgtattgat tgcgctgcgt atcaaagggg aaggggaagc agagatgctc ggcttttacg
    28381 aagccatgca aaatcacacc atcaagctga cgccgcctgc aggcaagccg atgccgattg
    28441 tcatccccag ttacaacggc gcgcgtaaac aggctaacct gacgccatta ttggcgattc
    28501 tgctgcataa actcggtttt cctgtggtgg ttcacggagt tagcgaagat ccaactcgcg
    28561 tgctgacaga aaccattttc gaactgatgg gtattacacc aacgttgcac ggcgggcagg
    28621 cgcaggcgaa gctcgacgaa catcaaccgg tgtttatgcc tgtcggcgcg ttttgcccgc
    28681 cgctggaaaa acaactggcg atgcgctggc ggatgggcgt gcgtaacagt gcacataccc
    28741 tggcgaaact ggcgacgcca tttgccgaag gtgaggcact gtgtctttcc agcgtttcgc
    28801 atccggaata cattggacgc gtcgcgaagt tctttagcga tatcggcggg tgggcgttac
    28861 taatgcatgg tactgaaggc gaagtgtatg ctaatccgca gcgctgcccg cagatcaatc
    28921 tcattgaccg tgaagggatg cgggtgctgt atgaaaaaca ggaccctgct ggtagcgagt
    28981 tactgccaca agcaaaagat ccggaaacca cggcgcagtg gactgagcgt tgcctggctg
    29041 gcagcgaagc gattcccgaa tcgctgaaaa tccagatggc ttgctgcctg gtggctacgg
    29101 gtgaagcggc aactatcagc gatggcctgg cgcgcgttaa tcaggcattt taatttttat
    29161 ctcagccgga tgagccacgt catccggctt ttaccccacc gcaatatgaa attcctgcat
    29221 ctttattgac cttcccgcgc ccggcgtgca gcataaaaaa acaacaaaca cataacataa
    29281 acaggagtta accatggaaa gtggtcatcg ctttgatgct cagacgctgc acagttttat
    29341 tcaggctgta tttcgtcaga tgggtagcga ggaacaagaa gcgaaattag ttgccgatca
    29401 tttaatcgcg gcaaacctgg cagggcatga ttctcatggt attggcatga tcccaagcta
    29461 tgtgcgttcc tggagtcagg ggcatctgca aattaaccat catgccaaaa tcgttaaaga
    29521 ggcgggggcg gcagtcacgc tcgatggcga tcgcgcattc ggtcaggtcg cggcacacga
    29581 agcgatggcg ctggggattg agaaagcgca tcagcacggt attgctgccg tggcgctaca
    29641 taactcgcat catatcggcc gtatcggtta ctgggcggag cagtgttcag cggcggggtt
    29701 tgtctctatc cactttgtta gcgtggtcgg tattccaatg gtcgcgccgt tccacggtcg
    29761 cgacagccgc tttggcacca atcccttctg tgtggttttc cctcgtaaag ataatttccc
    29821 gctgttgctt gattacgcca ccagcgccat tgcatttggc aaaacccgcg tcgcctggca
    29881 taaaggcgtc cccgtgccgc caggttgcct gattgacgtt aacggcgtgc cgacgaccaa
    29941 tccggcggta atgcaggagt cgccgttggg gtcgctgttg acctttgccg aacataaagg
    30001 ctacgccctt gcagcgatgt gtgaaattct tggcggggcg ctttctggcg gtaaaacgac
    30061 gcatcaggaa acgttgcaaa ccagccccga tgccattctt aactgcatga ccactatcat
    30121 catcaacccg gaactgttcg gcgcgccgga ttgtaacgcg cagaccgaag cctttgccga
    30181 gtgggtgaaa gcctcgccgc atgatgacga taagccgatt ttgctaccgg gcgagtggga
    30241 agtgaacacg cgtcgcgaac ggcaggagca ggggattcca ttggatgcgg gaagctggca
    30301 ggccatttgt gatgcggcgc ggcagattgg tatgtcggaa gagacgttgc aggctttctg
    30361 tcagcagtta gccagctaaa aaaaagcccg tccagtggcg gacgggcaaa caagggtaac
    30421 ataggatcaa tgagggttag agcatatgcg tctgtcggca aacagacagg gaaatacttg
    30481 tgctggacgt agcgtaaacg cctgatccgt ccaacggttc ggattttgta ggcctgataa
    30541 gacgcggtag cgtcgcatca ggcatctgtt gccggatgcg gcataaacgc cttatccgtc
    30601 ctacgaatta cttataaatt accgcagtac cgcttaattt attgttgttg gtggcggaag
    30661 tgatgctgta tccactggca ccggctgccg ccgctttctc agccagtttc gcttccaggg
    30721 catcgagggt ggatgcgcca tcggcagaaa ccacgccgat tttattcatg ttctgtgcct
    30781 gggatgccgt taccggttcc gccgcgaaca cgccaaatga cagagttgaa agagccatag
    30841 cagcaacaac agtattgata gttttcataa ttaatctctc gcaggtgatt tttgtataag
    30901 tgacgttgtt tcgtcgatgt gatgagtatc acgttttttt acgagagata aaatcgaaga
    30961 gaattgaccg ccttgttcaa ataaattgat tgatatctaa catactgata aaccatttat
    31021 ttgttgtact tatggacttg ttttatgccg cgtggcagtc acagcagcgt taaaggcacg
    31081 gaatgccacg caaaatacaa attatactcg ctatgcatct cgccagttgt agttaacgta
    31141 actgtctgtc tttcgaacct ctgcgattat atcctgtata cgctggtttt tgtaaatctt
    31201 tctcctgctg acaatgaata cataagcgca cgccaggaat ggcttcccga cgggcttgcg
    31261 ggatgggggc accgcactct tcacattcat acaggctttc gccacgcgga atttcacccc
    31321 gagcgcgggc aatcgcatct tcaattgtac tgttgatctg ttcgttgacg gcgtcatcgt
    31381 tagcccaagc ggatgccata tcgtcctccc catatcaatg cttgtacagt taagtgtagc
    31441 taatataggg acgaacctgg gcagttcaag cgtcagatct ccgaccattc ccgcagcaga
    31501 ttatgataaa gattgagcag cgacaggatc tcttcatttt caccatagcg gcttttcagc
    31561 gactgaatat ttttgtccag ttcaaacagc atggcgcgct ttttatcatc gcggatcatc
    31621 gactggatcc acataaatga tgccactcgt acgccgcggg ttacgggtgt cacgcaatgc
    31681 aggctgctgg agggatacaa caccagatca cctgccggga gttttacccg atgttgtcca
    31741 aaggtgtcat taacgaccag ttcgccgccg tcgtagcttt gtggatcgct taaaaacagc
    31801 gtggcagaaa ggtcagtgcg catccagccg ttttgtggat ggctgcgtac tgcgccatcc
    31861 acatggaaac cataggtttc attgttctga tagcgattaa acagcggcgt ggaaagggta
    31921 cgcggcaagg cagcggcaaa gaacaatgca tgttggttaa ccgcgttcag cacctcattt
    31981 tgcagtgcgg cgtataacgc gctgcgggtg tctacttgct gattgttctt aacttgtgca
    32041 ccttgtgctc cggtggtgac gcgtccatcc acccattcgg cttgttccag ttgttcgcga
    32101 aaatgggcga cgtcctgtgg cgataacacg ccgggaatgt ggtacatcat acaagttctc
    32161 caaaaagtgg ggcctgcgcc ccacatctga atcagaaatg catattggct gtgagcaaga
    32221 aggttcttgg ctcgcccggg tgataacggt agccgctctt gttgattgag gcgacgtaat
    32281 cggtatcaaa caggttgtaa acgtttagct ggaagtcgag attgcgatta acccgatacc
    32341 ccagtttggc atcggcgacc cagtaacctt ctgtaaatgc tggcgttccc accgcgccgt
    32401 ctgaaccttt atgtatactg ccgatatagc gtgcgcccgc gccaacagag atatcatcgg
    32461 ttgcctgata ttggctccat aaggtgaagg cgtgctccgg ggtatacggc agcgatgagg
    32521 aaccatcctg ggcaacatct ttgccgtttt tgatggttgc tttttgctgg gtatagccgc
    32581 caatcacctg ccacgcggga gtgatattcc ctgccacgga tatctcatag ccttcgacgc
    32641 gtttcttacc gtattgcgag taagtcccgt catcattttg ctcaacttca ttttcgatat
    32701 cagtacggaa cagcgcggcg gtgagcaaca ggcgtttatc cagaacctgc catttggtgc
    32761 caatctcgct ggtgttggct ttttgcggtt taaaatcggt gcggttggca ctgttaccac
    32821 tgccagactg cgcaagggcg aagttgttgc cgcccggagg ctgctgggaa acggcatagt
    32881 taatatagac attgccgttt tccgtcaggt ggtacagcgc cccggctttc cagttcacca
    32941 gattgcccga cttggcggtg tcgacggtgg tgactggaga accttttgcc acaccagctg
    33001 ggcaggtgat ggcaccgcgt ccgctgccgc cgcaggcggt ggcactgtca tattcagtat
    33061 gataattatc cagacggatc ccgccgttca gctcaaaatc acgggtgatt tgcagcgtat
    33121 cgaaggcgta aattgcgaag gtatccgtct gaccattggc gtttgcgccg ttgcgcgtca
    33181 ggccgccggg atgaattctg ctgtcaggat gataaatatt taccgccggt aacgtcaccg
    33241 gattaacgcc atagttagtc tgcgtttcac gggtaaattc cacgccggta ctgacatcat
    33301 gaccgataga accggtatag aacgtcgagg tcaggttggt ctggttggtg agaattttat
    33361 tactcacatc tttggtattc gccgtgcgtg actaggtcca gctattcaca tcgctggtgg
    33421 gctgagtaat attcgacgcc ccgcccataa tcgccgtcat caggtaatcc tgctttacgc
    33481 gcgaccaacg ggtagtattg cgaatggtgg tgttatcgtt gatgtcgtgc tcaaaacgca
    33541 tggtggcggt gtcggtggtc gaatcgtcgt aatcggaatc cgtgccgtaa aagttatgag
    33601 tatcaacttt tccggaatga ttcagggccg ccgctcccgc agatggggca gaatagcccg
    33661 gcaaaccgat ggtcggaatg ccgccgtctg gcgtgttgtg ctgagtgaca tgcagataat
    33721 taagatacaa acgatttgct gtaccaaggc caaaagcgac agaaggggcg acgccgtaac
    33781 gctcattttt gactttgtcg cgtccggcat cgtgggtttt ttcgcccatc acattcaggc
    33841 gcaccgcggt agtatcacca atgacctgat tgacgtccag cgtgccgcgg cggaaccagg
    33901 cgctgccaat act
//
LOCUS       DAJTRC010000038        33776 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.038, whole genome shotgun sequence.
ACCESSION   DAJTRC010000038 DAJTRC010000000
VERSION     DAJTRC010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33776)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33776)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33776
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.038"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..235)
                     /locus_tag="OO357_001920"
     CDS             complement(<1..235)
                     /locus_tag="OO357_001920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5886879.1"
                     /translation="MVTFETVMEIKILHKQGMSSRTIARELGLSRNTVKRYLQAKSEP
                     PKYTPRPAVASLLDEYRDYIRQRIADAHPYKIPA"
     gene            851..988
                     /locus_tag="OO357_001921"
     CDS             851..988
                     /locus_tag="OO357_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001102512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886880.1"
                     /translation="MNVQAIFILMVVQLFGKEIAVTIEELQAVLVPHRLLSQLVKFRS
                     A"
     gene            1588..2367
                     /gene="ipaJ"
                     /locus_tag="OO357_001922"
     CDS             1588..2367
                     /gene="ipaJ"
                     /locus_tag="OO357_001922"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858255.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     protease IpaJ"
                     /protein_id="HCR5886881.1"
                     /translation="MSEQRKPCKRGCIHTGVMLYGVLLQGAIPREYMISHQTDVRVNE
                     NRVNEQGCFLARKQMYDNSCGAASLLCAAKELGVDKIPQYKGSMSEMTRKSSLDLDNR
                     CERDLYLITSGNYNPRIHKDNIADAGYSMPDKIVMATRLLGLNAYVVEESNIFSQVIS
                     FIYPDARDLLIGMGCNIVHQRDVLSSNQRVLEAVAVSFIGVPVGLHWVLCRPDGSYMD
                     PAVGENYSCFSTMELGARRSNSNFIGYTKIGISIVITNEAL"
     gene            complement(3314..4243)
                     /gene="virB"
                     /locus_tag="OO357_001923"
     CDS             complement(3314..4243)
                     /gene="virB"
                     /locus_tag="OO357_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence regulon transcriptional activator
                     VirB"
                     /protein_id="HCR5886882.1"
                     /translation="MVDLCNDLLSIKEGQKKEFTLHSGNKVSFIKAKIPHKRIQDLTF
                     VNQKTNVRDQESLTEESLADIIKTIKLQQFFPVIGREIDGRIEILDGTRRRASAIYAG
                     ADLEVLYSKEYISTLDARKLANDIQTAKEHSIRELGIGLNFLKVSGMSYKDIAKKENL
                     SRAKVTRAFQAASVPQEIISLFPIASELNFNDYKILFNYYKGLEKANESLSSTLPILK
                     EEIKDLDTNLPPDIYKKEILNIIKKSKNRKQNPSLKVDSLFISKDKRTYIKRKENKTN
                     RTLIFTLSKINKTVQREIDEAIRDIISRHLSSS"
     gene            complement(4460..4696)
                     /locus_tag="OO357_001924"
     CDS             complement(4460..4696)
                     /locus_tag="OO357_001924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HCR5886883.1"
                     /translation="MIKEKILSIVAFCYGIAYSKLSEETKFIEDLSADSLSLIEMLDM
                     ISFEFNLRIDESTLEHIITIGDLISVVKNSTKSI"
     gene            complement(4700..6601)
                     /gene="ipaA"
                     /locus_tag="OO357_001925"
     CDS             complement(4700..6601)
                     /gene="ipaA"
                     /locus_tag="OO357_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector invasin IpaA"
                     /protein_id="HCR5886884.1"
                     /translation="MHNVNNTQAPTFLYKATSPSSTEYSELKSKISDIHSSQTSLKTP
                     ASVSEKENFATSFNQKCLDFLFSSSGKEDVLRSIYSNSMNAYAKSEILEFSNVLYSLV
                     HQNGLNFENEKGLQKIVAQYSELIIKDKLSQDSAFGPWSAKNKKLHQLRQNIEHRLAL
                     LAQQHTSGEALSLGQKLLNTEVSSFIKNNILAELKLSNETVSSLKLDDLVDAQAKLAF
                     DSLRNQRKNTIDSKGFGIGKLSRDLNTVAVFPELLRKVLNDILEDIKDSHPIQDGLPT
                     PPEDMPDGGPTPGANEKTSQPVIHYHINNDNRTYDNRVFDNRVYDNSYHENPENDAQS
                     PTSQTNDLLSRNGNSLLNPQRALVQKVTSVLPHSISDTVQTFANNSALEKAFNHTPDN
                     SDGIGSDLLTTSSQERSANNSLSRGHRPLNIQNSSTTPPLHPEGVTSSNDNSSDTTKS
                     SASLSHRVASQINKFNSNTDSKVLQTDFLSRNGDTYLTRETIFEASKKVTNSLSNLIS
                     LIGTKSGTQERELQEKSKDITKSTTEHRINNKLKVTDANIRNYVTETNADTIDKNHAI
                     YEKAKEVSSALSKVLSKIDDTSAELLTDDISDLKNNNDITAENNNIYKAAKDVTTSLS
                     KVLKNINKD"
     gene            complement(6610..7608)
                     /gene="ipaD"
                     /locus_tag="OO357_001926"
     CDS             complement(6610..7608)
                     /gene="ipaD"
                     /locus_tag="OO357_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle tip complex
                     protein IpaD"
                     /protein_id="HCR5886885.1"
                     /translation="MNITTLTNSISTSSFSPNNTNGSSTETVNSDIKTTTSSHPVSSL
                     TMLNDTLHNIRTTNQALKKELSQKTLTKTSLEEIALHSSQISMDVNKSAQLLDILSRH
                     EYPINKDARELLHSAPKEAELDGDQMISHRELWAKIANSINDINEQYLKVYEHAVSSY
                     TQMYQDFSAVLSSLAGWISPGGNDGNSVKLQVNSLKKALEELKEKYKDKPLYPANNTV
                     SQEQANKWLTELGGTIGKVSQKNGGYVVSINMTPIDNMLKSLDNLGGNGEVVLDNAKY
                     QAWNAGFSAEDETMKNNLQTLVQKYSNANSIFDNLVKVLSSTISSCTDTDKLFLHF"
     gene            complement(7659..8750)
                     /gene="ipaC"
                     /locus_tag="OO357_001927"
     CDS             complement(7659..8750)
                     /gene="ipaC"
                     /locus_tag="OO357_001927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858260.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS translocon subunit IpaC"
                     /protein_id="HCR5886886.1"
                     /translation="MEIQNTKPTQILYTDISTKQTQSSSETQKSQNYQQIAAHIPLNV
                     GKNPVLTTTLNDDQLLKLSEQVQHDSEIIARLTDKKMKDLSEMSHTLTPENTLDISSL
                     SSNAVSLIISVAVLLSALRTAETKLGSQLSLIAFDATKSAAENIVRQGLAALSSSITG
                     AVTQVGITGIGAKKTHSGISDQKGALRKNLATAQSLEKELAGSKLGLNKQIDTNITSP
                     QTNSSTKFLGKNKLAPDNISLSTEHKTSLSSPDISLQDKIDTQRRTYELNTLSAQQKQ
                     NIGRATMETSAVAGNISTSGGRYASALEEEEQLISQASSKQAEEASQVSKEASQATNQ
                     LIQKLLNIIDSINQSKNSTASQIAGNIRA"
     gene            complement(8770..10512)
                     /gene="ipaB"
                     /locus_tag="OO357_001928"
     CDS             complement(8770..10512)
                     /gene="ipaB"
                     /locus_tag="OO357_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system translocon subunit
                     IpaB"
                     /protein_id="HCR5886887.1"
                     /translation="MHNVSTTTTGFPLAKILASTELGDNTIQAANDAANKLFSLTIAD
                     LTANQNINTTNAHSTSNILIPELKAPKSLNASSQLTLLIGNLIQILGEKSLTALTNKI
                     TAWKSQQQARQQKNLEFSDKINTLLSETEGLTRDYEKQINKLKNADSKIKDLENKINQ
                     IQTRLSELDPESPEKKKLSREEIQLTIKKDAAVKDRTLIEQKTLSIHSKLTDKSMQLE
                     KEIDSFSAFSNTASAEQLSTQQKSLTGLASVTQLMATFIQLVGKNNEESLKNDLALFQ
                     SLQESRKTEMERKSDEYAAEVRKAEELNRVMGCVGKILGALLTIVSVVAAAFSGGASL
                     ALAAVGLALMVTDAIVQAATGNSFMEQALNPIMKAVIEPLIKLLSDAFTKMLEGLGVD
                     SKKAKMIGSILGAIAGALVLVAAVVLVATVGKQAAAKLAENIGKIIGKTLTDLIPKFL
                     KNFSSQLDDLITNAVARLNKFLGAAGDEVISKQIISTHLNQAVLLGESVNSATQAGGS
                     VASAVFQNSASTNLADLTLSKYQVEQLSKYISEAIEKFGQLQEVIADLLASMSNSQAN
                     RTDVAKAILQQTTA"
     gene            complement(10518..10985)
                     /gene="ipgC"
                     /locus_tag="OO357_001929"
     CDS             complement(10518..10985)
                     /gene="ipgC"
                     /locus_tag="OO357_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SycD/LcrH family type III secretion system
                     chaperone IpgC"
                     /protein_id="HCR5886888.1"
                     /translation="MSLNITENESISTAVIDAINSGATLKDINAIPDDMMDDIYSYAY
                     DFYNKGRIEEAEVFFRFLCIYDFYNVDYIMGLAAIYQIKEQFQQAADLYAVAFALGKN
                     DYTPVFHTGQCQLRLKAPLKAKECFELVIQHSNDEKLKIKAQSYLDAIQDIKE"
     gene            complement(11042..11668)
                     /gene="ipgB1"
                     /locus_tag="OO357_001930"
     CDS             complement(11042..11668)
                     /gene="ipgB1"
                     /locus_tag="OO357_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB1"
                     /protein_id="HCR5886889.1"
                     /translation="MQILNKILPQVEFAIPRPSFDSLSRNKLVKKILSVFNLKQRFPQ
                     KNFGCPVNINKIRDSVIDKIKDSNSGNQLFCWMSQERTTYVSSMINRSIDEMAIHNGV
                     VLTSDNKKNIFAAIEKKFPDIKLDEKSAQTSISHTALNEIASSGLRAKILKRYSSDMD
                     LFNTQMKDLTNLVSSSVYDKIFNESTKVLQIEISAEVLKAVYRQSNTN"
     gene            complement(11684..12073)
                     /gene="ipgA"
                     /locus_tag="OO357_001931"
     CDS             complement(11684..12073)
                     /gene="ipgA"
                     /locus_tag="OO357_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgA"
                     /protein_id="HCR5886890.1"
                     /translation="MCRKLYDKLYEITGAKLDFNDKNQAFILLEEQIPVCITDNDEYI
                     FLTGLLNEHELFTENIINPEHILILNYSLSRDYGSSICLLPDTHQCVLTKKHYKKYLS
                     PDELIESLYEFLFCIKLTIANITSEVN"
     gene            complement(12086..13570)
                     /gene="icsB"
                     /locus_tag="OO357_001932"
     CDS             complement(12086..13570)
                     /gene="icsB"
                     /locus_tag="OO357_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector IcsB"
                     /protein_id="HCR5886891.1"
                     /translation="MILKISNFIDASNTKGPIRVEDTEHGPILIAQKFNLKDLFFRTL
                     STINAKINSQILNEQLKNYRLENQKSLLLFLNTLASEKSAESAFAAYEAAKNSIQHSF
                     TGRDIKLMLNTAERFHGIGTAKNLERHLVFRCWGNRGITHLGHTSISIKNNLLQEPTH
                     TYLSWYPGGNVTKDTEINYLFEKRSGYSVDTYKQDKLNMISDQTAERLDAGQEVRNLL
                     NSKQDQNNNKKIFFPRANQKKDPYGYWGVSADKVYIPLSGDNKTKDGKISHNLFGLDE
                     TNMSKFICKKKADAFRQLANYKLISKSENCAGMALNVLKAGNSEIYFPLPDVKLVATP
                     NDVYAYANKVRQRIESLNQSYNEIMKYIESDFDLSRLTQLRRSYLKSFNKINLIHTPK
                     TFKPLSISLYKHPTENVSSEDFDAVINACHSYLVKSAPSNMTRVLNELKTEATDKKEE
                     IIEKSIKIIDYYNSLKSPDLGTKLYIHDLLQINKLLLNNSHSNI"
     gene            13884..15500
                     /locus_tag="OO357_001933"
     CDS             13884..15500
                     /locus_tag="OO357_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector inositol
                     phosphate phosphatase"
                     /protein_id="HCR5886892.1"
                     /translation="MHITNLGLHQVSFQSGDSYKGAEETGKHKGVSVISYQRVKNGER
                     NKGIEALNRLYLQNQTSLTGKSLLFARDRAEVFCEAIKLAGGDTSKIKAMMERLDTYK
                     LGEVNKRHINELNKVISEEIRAQLGIKNKKELQTKIKQIFTDYLNNKNWGPVDKNISH
                     HGKNYGFQLTPASHMKIGNKNIFVKEYNGKGICCASTRESDHIANMWLSKVVDDEGKE
                     IFSGIRHGVISAYGLKKNSSERAVAARNKAEELVSAALYSRPELLSQALSGKTVDLKI
                     VSTSLLTPTSLTGGEESMLKDQVNALKGLNSKRGKPTKLLIRNSDGLLKEVSVNLKVV
                     TFNFGVNELALKMGLGWRNVDKLNDESICSLLGDNFLKNGVIGGWAAEAIEKNPPCKN
                     DVIYLANQIKEIVTKKLQKNDNGEPYKLSQRMTLLAYTIGAVPCWNCKSGKDRTGMQD
                     AEIKREIIRKHETGQFSQLNSKLSSEEKRLFSTILMNSGNMEIQEMNTGVPGNKVMKK
                     LPLSSLELSYSERIGDPKIWNMVKGYSSFV"
     gene            15510..15872
                     /gene="ipgE"
                     /locus_tag="OO357_001934"
     CDS             15510..15872
                     /gene="ipgE"
                     /locus_tag="OO357_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone IpgE"
                     /protein_id="HCR5886893.1"
                     /translation="MEDLADVICRALGIPLIDIDDQAIMLDDDVLIYIEKEGDSINLL
                     CPFCALPENINDLIYALSLNYSEKICLATDDEGGNLIARLDLTGINEFEDVYVNTEYY
                     ISRVRWLKDEFARRMKGY"
     gene            15872..16330
                     /gene="ipgF"
                     /locus_tag="OO357_001935"
     CDS             15872..16330
                     /gene="ipgF"
                     /locus_tag="OO357_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system lytic transglycosylase
                     IpgF"
                     /protein_id="HCR5886894.1"
                     /translation="MSRFVFILLCFIPYLGRADCWDKAGERYNIPSSLLKAIAEKESG
                     FNKSAVNVNNNGSKDYGIMQINDFHSKRLREMGYSEEMLISHPCLSVHYAAKLLNEFM
                     MMYGRGWEAVGAYNAGTSPKKKKERLKYAEDIYRRYLRIAAESKQNNRRI"
     gene            16339..17454
                     /gene="mxiG"
                     /locus_tag="OO357_001936"
     CDS             16339..17454
                     /gene="mxiG"
                     /locus_tag="OO357_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiG"
                     /protein_id="HCR5886895.1"
                     /translation="MSEAKNSNLAPFRLLVKLTNGVGDEFPLYYGNNLIVLGRTIETL
                     EFGNDNFPENIIPVTDSKSDGIIYLTISKDNICQFSDEKGEQIDINSQFNSFEYDGIS
                     FHLKNMREDKSRGHILNGMYKNHSVFFFFAVIVVLIIIFFLSLKKDEVKEIAEIIDDK
                     RYGIVNTGQCNYILAETQNDAVWASVALNKTGFTKCRYILVSNKEINRIQQYINQRFP
                     FINLYVLNLVSDKAELLVFLSKERNSSKDTELDKLKNALIVEFPYIKNIKFNYLSDHN
                     ARGDAKGIFTKVNVQYKEICENNKVTYSVREELTDEKLELINRLISEHKNIYGDQYIE
                     FSVLLIDDDFKGKSYLNSKDSYVMLNDKHWFFLDKNK"
     gene            17462..17713
                     /gene="mxiH"
                     /locus_tag="OO357_001937"
     CDS             17462..17713
                     /gene="mxiH"
                     /locus_tag="OO357_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle filament
                     protein MxiH"
                     /protein_id="HCR5886896.1"
                     /translation="MSVTVPNDDWTLSSLSETFDDGTQTLQGELTLALDKLAKNPSNP
                     QLLAEYQSKLSEYTLYRNAQSNTVKVIKDVDAAIIQNFR"
     gene            17726..18019
                     /gene="mxiI"
                     /locus_tag="OO357_001938"
     CDS             17726..18019
                     /gene="mxiI"
                     /locus_tag="OO357_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system inner rod protein
                     MxiI"
                     /protein_id="HCR5886897.1"
                     /translation="MNYIYPVNQVDIIKASDFQSQEISSLEDVVSAKYSDIKMDTDIQ
                     VSQIMEMVSNPESLNPESLAKLQTTLSNYSIGVSLAGTLARKTVSAVETLLKS"
     gene            18025..18750
                     /gene="mxiJ"
                     /locus_tag="OO357_001939"
     CDS             18025..18750
                     /gene="mxiJ"
                     /locus_tag="OO357_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctJ family type III secretion inner membrane
                     ring lipoprotein Mxi"
                     /protein_id="HCR5886898.1"
                     /translation="MIRYKGFILFLLLMLIGCEQREELISNLSQRQANEIISVLERHN
                     ITARKVDGGKQGISVQVEKGTFASAVDLMRMYDLPNPERVDISQMFPTDSLVSSPRAE
                     KARLYSAIEQRLEQSLVSIGGVISAKIHVSYDLEEKNISSKPMHISVIAIYDSPKESE
                     LLVSNIKRFLKNTFSDVKYENISVILTPKEEYVYTNVQPVKEVKSEFLTNEVIYLFLG
                     MAVLVVILLVWAFKTGWFKRNKI"
     gene            18747..19274
                     /locus_tag="OO357_001940"
     CDS             18747..19274
                     /locus_tag="OO357_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion apparatus protein OrgA/MxiK"
                     /protein_id="HCR5886899.1"
                     /translation="MIRMDGIYKKYLSIIFDPAFYINRNRLNLPSELLENGVIRSEIN
                     NLIINKYDLNCDIEPLSGVTAMFVANWNLLPAVAYFIGSQESRLINHSEMVISYYGGK
                     ISKQGEAAIRSGFWHLIAWKENISVGIYERINLLFNPIALEGNYTPVERNLSRLNEGM
                     QYAKRHFTGIQTSCL"
     gene            19216..19911
                     /gene="mxiN"
                     /locus_tag="OO357_001941"
     CDS             19216..19911
                     /gene="mxiN"
                     /locus_tag="OO357_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mxi-Spa type III secretion system linker protein
                     MxiN"
                     /protein_id="HCR5886900.1"
                     /translation="MKVCNMQKGTLPVSRHHAYDGVVIKRIEKELCKTIKDRDTESKK
                     KAICVIKEATKKAESLRIDAVCDGYQIGIQTAFEHIIDYICEWKLKQNENRRNIEDYI
                     TSLLSENLHDERIISTLLEQWLSSLRNTVTELKVVLPKCNLALRKKLELDLHKYRSDV
                     KIILKYSEGNNYIFCSGNQVVEFSPQDVISGVKIELAEKLTKNDKKYFKELAHKKLRQ
                     IAEDLLKENPVND"
     gene            19904..20311
                     /gene="mxiM"
                     /locus_tag="OO357_001942"
     CDS             19904..20311
                     /gene="mxiM"
                     /locus_tag="OO357_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein MxiM"
                     /protein_id="HCR5886901.1"
                     /translation="MINQINASNALQQRLNSEEVVNLNERLSSSQSFDEDIIYEIMQY
                     FSQSELNSIDNDELHNKIEQLFNSRFPYLTAAQKSSLLNKLIDANQYVDLHEGFYASL
                     SIYNNIDFYIKTTTFDSLISVFEAGREADDSTW"
     gene            20295..20723
                     /gene="mxiM"
                     /locus_tag="OO357_001943"
     CDS             20295..20723
                     /gene="mxiM"
                     /locus_tag="OO357_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858276.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system pilotin MxiM"
                     /protein_id="HCR5886902.1"
                     /translation="MIRHGSNKLKIFILSILLLTLSGCALKSSSNSEKEWHIVPVSKD
                     YFSIPNDLLWSFNTTNKSINVYSKCISGKAVYSFNAGKFMGNFNVKEVDGCFMDAQKI
                     AIDKLFSMLKDGVVLKGNKINDTILIEKDGEVKLKLIRGI"
     gene            20853..21485
                     /gene="mxiE"
                     /locus_tag="OO357_001944"
     CDS             20853..21485
                     /gene="mxiE"
                     /locus_tag="OO357_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system transcriptional
                     activator MxiE"
                     /protein_id="HCR5886903.1"
                     /translation="MEGFFFVRNQNIKFSDNVNYHYRFNINSCAKFLAFWDYFSGALV
                     EHSHAEKCIHFYHENDLRDSCNTESMLDKLMLRFIFSSDQNVSNALAMIRMTESYHLV
                     LYLLRTIEKEKEVRIKSLTEHYGVSEAYFRSLCRKALGAKVKEQLNTWRLVNGLLDVF
                     LHNQTITSAAMNNGYASTSHFSNEIKTRLGFSARELSNITFLVKKINEKI"
     gene            21472..23172
                     /gene="mxiD"
                     /locus_tag="OO357_001945"
     CDS             21472..23172
                     /gene="mxiD"
                     /locus_tag="OO357_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system secretin MxiD"
                     /protein_id="HCR5886904.1"
                     /translation="MKKFNIKSLTLLIVLLPLIVNANNIDSHLLEQNDIAKYVAQSDT
                     VGSFFERFSALLNYPIVVSKQAAKKRISGEFDLSNPEEMLEKLTLLVGLIWYKDGNAL
                     YIYDSGELISKVILLENISLNYLIQYLKDANLYDHRYPIRGNISDKTFYISGPPALVE
                     LVANTATLLDKQVSSIGTDKVNFGVIKLKNTFVSDRTYNMRGEDIVIPGVATVVERLL
                     NNGKALSNRQAQNDPMPPFNITQKVSEDSNDFSFSSVTNSSILEDVSLIAYPETNSIL
                     VKGNDQQIQIIRDIITQLDIAKRHIELSLWIIDIDKSELNNLGVNWQGTASFGDSFGA
                     SFNMSSSASISTLDGNKFIASVMALNQKKKANVVSRPVILTQENIPAIFDNNRTFYVS
                     LVGERNSSLEHVTYGTLINVIPRFSSRGQIEMSLTIEDGTGNSQSNYNYNNENTSVLP
                     EVGRTKISTIARVPQGKSLLIGGYTHETNSNEIISIPFLSSIPVIGNVFKYKTSNISN
                     IVRVFLIQPREIKESSYYNTAEYKSLISEREIQKTTQIIPSETTLLEDEKSLVSYLNY
                     "
     gene            23191..24258
                     /gene="mxiC"
                     /locus_tag="OO357_001946"
     CDS             23191..24258
                     /gene="mxiC"
                     /locus_tag="OO357_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system gatekeeper subunit
                     MxiC"
                     /protein_id="HCR5886905.1"
                     /translation="MLDVKNTGVFSSAFIDKLNAMTNSDDGDETADAELDSGLANSKY
                     IDSSDEMASALSSFINRRDLEKLKGTNSDSQERILDGEEDEINHKIFDLKRTLKDNLP
                     LDRDFIDRLKRYFKDPSDQVLALRELLNEKDLTAEQVELLTKIINEIISGSEKSVNAG
                     INSAIQAKLFGNKMKLEPQLLRACYRGFIMGNISTTDQYIEWLGNFGFNHRHTIVNFV
                     EQSLIVDMDSEKPSCNAYEFGFVLSKLIAIKMIRTSDVIFMKKLESSSLLKDGSLSAE
                     QLLLTLLYIFQYPSESEQILTSVIEVSRASHEDSVVYQTYLSSVNESPHDIFKSESER
                     EIAINILRELVTSAYKKELSR"
     gene            24271..26331
                     /gene="mxiA"
                     /locus_tag="OO357_001947"
                     /gene_synonym="virH"
     CDS             24271..26331
                     /gene="mxiA"
                     /locus_tag="OO357_001947"
                     /gene_synonym="virH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein MxiA"
                     /protein_id="HCR5886906.1"
                     /translation="MIQSFLKQVSTKPELIILVLMVMIIAMLIIPLPTYLVDFLIGLN
                     IVLAILVFMGSFYIERILSFSTFPSVLLITTLFRLALSISTSRLILVDADAGKIITTF
                     GQFVIGDSLAVGFVIFSIVTVVQFIVITKGSERVAEVAARFSLDGMPGKQMSIDADLK
                     AGIIDAAGAKERRSILERESQLYGSFDGAMKFIKGDAIAGIIIIFVNLIGGISVGMSQ
                     HGMSLSGALSTYTILTIGDGLVSQIPALLISISAGFIVTRVNGDSDNMGRNIMSQIFG
                     NPFVLIVTSALALAIGMLPGFPFFVFFLIAVTLTALFYYKKVVEKEKSLSESDSSGYT
                     GTFDIDNSHDSSLAMIENLDAISSETVPLILLFAENKINANDMEGLIERIRSQFFIDY
                     GVRLPTILYRTSNELKVDDIVLLINEVRADSFNIYFDKVCITDENGDIDALGIPVVST
                     SYNERVISWVDVSYTENLTNIDAKIKSAQDEFYHQLSQALLNNINEIFGIQETKNMLD
                     QFENRYPDLLKEVFRHVTIQRISEVLQRLLGENISVRNLKLIMESLALWAPREKDVIT
                     LVEHVRASLSRYICSKIAVSGEIKVVMLSGYIEDAIRKGIRQTSGGSFLNMDIEVSDE
                     VMETLAHALRELRNAKKNFVLLVSVDIRRFVKRLIDNRFKSILVISYAEIDEAYTINV
                     LKTI"
     gene            26344..26745
                     /gene="spa15"
                     /locus_tag="OO357_001948"
                     /gene_synonym="spaK"
     CDS             26344..26745
                     /gene="spa15"
                     /locus_tag="OO357_001948"
                     /gene_synonym="spaK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system chaperone Spa15/SpaK"
                     /protein_id="HCR5886907.1"
                     /translation="MSNINLVQLVRDSLFTIGCPPSIITDLDSHSAITISLDSMPAIN
                     IALVNEQVMLWANFDAPSDVKLQSSAYNILNLMLMNFSYSINELVELHRSDEYLQLRV
                     VIKDDYVHDGIVFAEILHEFYQRMEILNGVL"
     gene            26749..28041
                     /gene="spaL"
                     /locus_tag="OO357_001949"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
     CDS             26749..28041
                     /gene="spaL"
                     /locus_tag="OO357_001949"
                     /gene_synonym="mxiB"
                     /gene_synonym="spa47"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system ATPase SpaL"
                     /protein_id="HCR5886908.1"
                     /translation="MSYTKLLTQLSFPNRISGPILETSLSDVSIGEICNIQAGIESNE
                     IVARAQVVGFHDEKTILSLIGNSRGLSRQTLIKPTAQFLHTQVGRGLLGAVVNPLGEV
                     TDKFAVTDNSEILYRPVDNAPPLYSERAAIEKPFLTGIKVIDSLLTCGEGQRMGIFAS
                     AGCGKTFLMNMLIEHSGADIYVIGLIGERGREVTETVDYLKNSEKKSRCVLVYATSDY
                     SSVDRCNAAYIATAIAEFFRTEGHKVALFIDSLTRYARALRDVALAAGESPARRGYPV
                     SVFDSLPRLLERPGKLKAGGSITAFYTVLLEDDDFADPLAEEVRSILDGHIYLSRNLA
                     QKGQFPAIDSLKSISRVFTQVVDEKHRIMAAAFRELLSEIEELRTIIDFGEYKPGENA
                     SQDKIYNKISVVESFLKQDYRLGFTYEQTMELIGETIR"
     gene            28134..28472
                     /gene="spa13"
                     /locus_tag="OO357_001950"
                     /gene_synonym="spaM"
     CDS             28134..28472
                     /gene="spa13"
                     /locus_tag="OO357_001950"
                     /gene_synonym="spaM"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858283.1"
                     /note="Not similar to SpaM from Salmonella T3SS; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein Spa13"
                     /protein_id="HCR5886909.1"
                     /translation="MEALDKRIIYFLQLENDLEPVGAQSVSQLFNTRRKIAIVKKHII
                     QYQSERILLKGRIEEIQKDIDEANASKRKLLHKESKICKRIGLIKRNNFAKQLILDEL
                     SQEDMKYGIR"
     gene            28459..29337
                     /gene="spa32"
                     /locus_tag="OO357_001951"
     CDS             28459..29337
                     /gene="spa32"
                     /locus_tag="OO357_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system needle length
                     regulator Spa32"
                     /protein_id="HCR5886910.1"
                     /translation="MALDNINLNFSSDKQIEKCEKLSSIDNIDSLVLKKKRKVEIPEY
                     SLIASNYFTIDKHFEHKHDKGEIYSGIKNAFELRNERATYSDIPESMAIKENILIPDQ
                     DIKAREKINIGDMRGIFSYNKSGNADKNFERSHTSSVNPDNLLESDNRNGQIGLKNHS
                     LSIDKNIADIISLLNGSVAKSFELPVMNKNTADITPSMSLQEKSIVENDKNVFQKNSE
                     MTYHFKQWGAGHSVSISVESGSFVLKPSDQFVGNKLDLILKQDAEGNYRFDSSQHNKG
                     NKNNSTGYNEQSEEEC"
     gene            29331..30212
                     /gene="spa33"
                     /locus_tag="OO357_001952"
                     /gene_synonym="spaO"
     CDS             29331..30212
                     /gene="spa33"
                     /locus_tag="OO357_001952"
                     /gene_synonym="spaO"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000944309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SctQ family type III secretion system ring
                     protein Spa33"
                     /protein_id="HCR5886911.1"
                     /translation="MLRIKHFDANEKLQILYAKQLCERFSIQTFKNKFTGSESLVTLT
                     SVCGDWVIRIDTLSFLKKKYEVFSGFSTQESLLHLSKCVFIESSSVFSIPELSDKITF
                     RITNEIQYATTGSHLCCFSSSLGIIYFDKMPVLRNQVSLDSLHHLLEFCLGSSNVRLA
                     TLKRIRTGDIIIVQKLYNLLLCNQVIIGDYIVNDNNEAKINLSESNGESEHTEVSLAL
                     FNYDDINVKVDFILLEKNMTINELKMYVENELFKFPDDIVKHVNIKVNGSLVGHGELV
                     SIEDGYGIEISSWMVKE"
     gene            30217..30867
                     /gene="spa24"
                     /locus_tag="OO357_001953"
                     /gene_synonym="spaP"
     CDS             30217..30867
                     /gene="spa24"
                     /locus_tag="OO357_001953"
                     /gene_synonym="spaP"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa24/SpaP"
                     /protein_id="HCR5886912.1"
                     /translation="MLSDMSLIATLSFFTLLPFLVAAGTCYIKFSIVFVMVRNALGLQ
                     QVPSNMTLNGIALIMALFVMKPIIEAGYENYLNGPQKFDTISDIVRFSDSGLMEYKQY
                     LKKHTDLELARFFQRSEEENADLKSAENNDYSLFSLLPAYALSEIKDAFKIGFYLYLP
                     FVVVDLVISSILLALGMMMMSPITISVPIKLVLFVALDGWGILSKALIEQYINIPA"
     gene            30882..31142
                     /gene="spaQ"
                     /locus_tag="OO357_001954"
     CDS             30882..31142
                     /gene="spaQ"
                     /locus_tag="OO357_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein SpaQ"
                     /protein_id="HCR5886913.1"
                     /translation="MSDIVYMGNKALYLILIFSLWPVGIATVIGLSIGLLQTVTQLQE
                     QTLPFGIKLIGVSISLLLLSGWYGEVLLSFCHEIMFLIKSGV"
     gene            31142..31912
                     /gene="spa29"
                     /locus_tag="OO357_001955"
                     /gene_synonym="spaR"
     CDS             31142..31912
                     /gene="spa29"
                     /locus_tag="OO357_001955"
                     /gene_synonym="spaR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858288.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     protein Spa29/SpaR"
                     /protein_id="HCR5886914.1"
                     /translation="MDISSWFESIHVFLILLNGVFFRLAPLFFFLPFLNNGIISPSIR
                     IPVIFLVASGLITSGKVDIGSSVFEHVYFLMFKEIIVGLLLSFCLSLPFWIFHAVGSI
                     IDNQRGATLSSSIDPANGVDTSELAKFFNLFSAVVFLYSGGMVFILESIQLSYNICPL
                     FSQCSFRVSNILTFLTLLASQAVILASPVMIVLLLSEVLLGVLSRFAPQMNAFSVSLT
                     IKSLLAIFIIFICSSTIYFSKVQFFLGEHKFFTNLFVR"
     gene            31919..32947
                     /gene="spa40"
                     /locus_tag="OO357_001956"
                     /gene_synonym="spaS"
     CDS             31919..32947
                     /gene="spa40"
                     /locus_tag="OO357_001956"
                     /gene_synonym="spaS"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system export apparatus
                     switch protein Spa40/SpaS"
                     /protein_id="HCR5886915.1"
                     /translation="MANKTEKPTPKKLKDAAKKGQSFKFKDLTTVVIILVGTFTIISF
                     FSLSDVMLLYRYVIINDFEINEGKYFFAVVIVFFKIIGFPLFFCVLSAVLPTLVQTKF
                     VLATKAIKIDFSVLNPVKGLKKIFSIKTIKEFFKSILLLIILALTTYFFWINDRKIIF
                     SQVFSSVDGLYLIWGRLFKDIILFFLAFSILVIILDFVIEFILYMKDMMMDKQEIKRE
                     YIEQEGHFETKSRRRELHIEILSEQTKSDIRNSKLVVMNPTHIAIGIYFNPEIAPAPF
                     ISLIETNQCALAVRKYANEVGIPTVRDVKLARKLYKTHTKYSFVDFEHLDEVLRLIVW
                     LEQVENTH"
     gene            32960..33202
                     /locus_tag="OO357_001957"
     CDS             32960..33202
                     /locus_tag="OO357_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886916.1"
                     /translation="MCYMGVNFCNKIGIDQSEFEIESSIINSIANEVLNPISFLSNKD
                     IINVLLRKISSECDLVRKDIYRCALELVVEKTPDDL"
BASE COUNT        10520 a         4921 c         6783 g        11552 t
ORIGIN      
        1 ttgccgggat tttgtaagga tgagcatcgg cgatgcgttg acgaatataa tcccggtatt
       61 catccaggag tgaagcaaca gcaggtcgcg gcgtatattt tggcggctca gattttgcct
      121 gcaaataacg tttaacggta ttgcgggaga gccccagttc tctggcaatc gtccggctac
      181 tcattccctg cttgtgcagg attttaattt ccataactgt ctcaaaagtg accataagct
      241 ctcctgaatc aggagagcag attaccccct ggatctgatt tcaggcgttg ggtgtggatc
      301 actattgcac cgttcgtgac acttgttgac tacctgtgcg gcatctggta tcaaggtggc
      361 gtgaagccgg ttcaggttct catgcggaac gttggaacct atatcccccc gataccaaag
      421 gcgaaatgta agtgacaaaa tgcaatgaac acgttctccg ttaactgagg agcatggtga
      481 taaataaagg taaatgcaga aggtgtagag tctcgcagat ttctgacatc atagagccta
      541 acattagatg cataacgcta cagattgtag aatctctaca ggtgaaatat acactggttg
      601 gcgtttatgt cgcaaaacat gtcattcagg ttcatctcac caataaggat atgagtgaag
      661 tggaggataa gtgagtctgc tgtcagagtt ttctggtgta tgtcagtaac tgcgaaccat
      721 gtctgcttga tatgggagct tatggaggct ctctgtacaa ggttagggag ctaacaaagc
      781 agggataaaa agtccgcctg atgcaggcac ggttcgttaa atcttttgcc attggtaaca
      841 aaatggtgcg atgaatgttc aggctatctt tatcttgatg gtggttcagc tgtttggtaa
      901 agaaatcgct gtaacaatag aagaactgca ggcagtactg gtcccacatc gtttgctcag
      961 tcaactggtg aagttccgta gcgcgtaaat taatgctttg catgaaacgt tactggagtg
     1021 gatgaaagaa tacctgcata tatcattact gcgttggatt accagtacaa cagattgaat
     1081 gaattggaca tgttgataga cggcattgaa aagcagctta taagcatagt gcggcagagt
     1141 taattggaga gcacttcgcc ttctgagtat taagtaagca tggtggtacc tgcggatact
     1201 atctattcac tgtgctcggt cggcagcctt actgaccaag gagttggggg agttgataac
     1261 aaagttgaaa aaacagctct caaccaaggt ggttattgta acaatagcaa ataagctggt
     1321 gtgacctgta tcacaataac cgattatgga cgtaaatacg acaaaaatat gtcagtatca
     1381 ggcggtatta attgctgatg ctgatggaca ttaactctta ataaaagggt ggatgctgaa
     1441 aggttgttat ggtgacggac gtgatgacga cggaaaagag tatgaggttt tggatttaaa
     1501 gttcgttcgg ggaataagga cccggtcagc taattctcat agtagttcgc attgctggat
     1561 gcaatactgt gctgcaatta ctcgtcgatg tcggaacaac ggaagccttg caaacgggga
     1621 tgtattcata caggtgttat gttatatggg gttttattgc agggggctat accaagagaa
     1681 tatatgatat ctcatcagac tgatgtgagg gtaaatgaga atagagtaaa cgaacaaggt
     1741 tgttttttgg cgaggaagca gatgtatgac aattcttgtg gagctgccag tttactctgt
     1801 gctgcaaagg aattaggggt tgataaaata ccacaataca aagggtctat gtccgaaatg
     1861 acaaggaaga gttctcttga tttggataat cgttgtgagc gggatttata cttaataact
     1921 agtggtaatt ataatccgag aatacacaaa gataatatcg cggatgccgg ttattctatg
     1981 cctgataaga tagtcatggc tacgaggctt cttggattga atgcttatgt cgttgaagaa
     2041 agtaacattt tttcgcaagt aattagtttt atttatcccg atgcaaggga cttattaatt
     2101 ggcatgggat gtaatatagt acatcaacga gatgtgttat cttctaacca aagagttttg
     2161 gaagctgttg ctgtatcctt tattggagtt cctgtcggcc tccactgggt gttgtgtaga
     2221 cctgatggaa gctatatgga tccagctgta ggagaaaatt attcttgttt ttcaactatg
     2281 gaattaggtg caagaagaag taattctaac tttataggat atactaaaat aggtatttcc
     2341 atagttataa ctaatgaggc tttgtaaatt gtcgtttttt atctgtgaag gagcttgaat
     2401 attttggggt ggggggcata acaaatgata ttactgagta caaatttagc tatattgggc
     2461 ggattagttg tgtgggaaga cgaaatgttt ctcttgaaat aagtgaactc cctcaaggaa
     2521 tgattgtgtg cttaaatatt taagtgctgg taagtttgag aaggctgaca tattttgcag
     2581 agcaaacagt gattgacttg gatgatattt ttattttagt cactcgttgt aattggagta
     2641 tgctttattt tatttgaaaa tagatgtatt ttatacccgc aatttaaact gtaagcccta
     2701 tgaggggtat ggatattata agttactctt tgaccaagca taacttttgg ggttagaaaa
     2761 ataaatctct tgttaataat taatatgatt ttaaaatcaa acttgtttat aattataaaa
     2821 taatgtaagt cggtcatttt atgggaagat taggagaata aaacagtaat tatatttgag
     2881 aaatctgcta atatattctt aaagcttttt tgatactgtg aacgaagtct gttaatggtg
     2941 gattttttgt ttatcaccat gtaaaaaaaa ttgagtagta aaagcgttga ctgctaatcc
     3001 tcgctgtttt tattgaaacg attaaaaata cgctgaatat tttcggccag tcactcatat
     3061 tcacataaaa ttttgaccat catgccgcat cccttagttg cacaattcat tttcaatgat
     3121 atgaatatta ctaatttcac cagataattt tctcgtggtc agcagggtat gagttttcac
     3181 cgtaatttta aggaaacaca gtgcgtgggg attgaataac agatagccga cggaattacc
     3241 ccaccggctg aaagtcaagt tcaacgaaga tttttattat ctgaattggg cagtttacat
     3301 cagtgttcga tgtttatgaa gacgatagat ggcgagaaat tatatcccga atagcttcat
     3361 ctatttctct ctgaactgtt ttgtttattt tagataatgt aaatattaga gttctgtttg
     3421 ttttattctc ttttcttttt atgtaagtcc gtttgtcttt agaaataaat aacgagtcaa
     3481 cttttagcga agggttttgc tttctgtttt tgcttttctt tatgatgttt aaaatttctt
     3541 ttttatatat gtccgggggc aaatttgtat caaggtcttt tatttcttcc tttaatattg
     3601 gtagtgtaga actaagagat tcattagcct tttcaagtcc tttataataa ttgaataata
     3661 tcttgtagtc attaaagtta agttctgacg cgattggaaa tagagatatt atttcctgtg
     3721 gaacgcttgc tgcctgaaag gcacgagtga ctttcgcgcg agacagattc tcttttttgg
     3781 ctatgtcttt ataggacatc cctgatactt tcagaaaatt aagaccaata ccaagttctc
     3841 ggatgctatg ctcttttgct gtttgtatat cgtttgctag ttttctggca tcaagagtag
     3901 atatatattc ttttgaatat agaacttcaa gatctgctcc tgcatatatt gcagatgctc
     3961 ttctacgagt gccatccaga atttcaattc taccatcaat ctcccttcct attacaggga
     4021 agaattgttg tagctttata gtttttatga tatcggctaa tgattcttct gttagggatt
     4081 cttgatcgcg tacattcgtt ttttggttga cgaaggttaa atcttggatc cttttatgag
     4141 gaatcttggc tttgataaag gaaactttat taccagaatg gagtgtaaac tctttctttt
     4201 ggccttcctt tatacttaac aagtcgttgc acaaatccac catatcacac cctgtttatt
     4261 catattgatt tatattgaca aaagttaaat gcagtgtgag caagattgta tgtaactgtt
     4321 gatgatagtc aacggtagtc ttaaaaatta ctattttaat agctggattg atgcaattta
     4381 tctttattgg ataaaagaat aatattcttg tggagctctg atgtgagagc tccatagatg
     4441 aaaaaatgct tatcaattac tatattgatt ttgtactatt tttgactaca gaaataagat
     4501 ctccaatagt aataatgtgt tctaatgtag attcatctat acgtagattg aactcaaaac
     4561 taatcatatc cagcatttca atcagagaaa gagaatcggc agaaagatct tcaataaatt
     4621 tagtttcttc agatagtttg ctgtacgcta taccatagca aaaggccact attgataata
     4681 ttttttcttt tatcatattt taatccttat tgatattctt taatactttt gatagggaag
     4741 tggttacatc ttttgctgct ttatatatat tattgttttc agctgtaata tcattgttat
     4801 tttttaaatc agatatatca tctgtaagta attctgcaga ggtatcgtca atttttgaca
     4861 ataccttgct gagggcgcta gatacttctt ttgccttttc atagatcgca tgatttttat
     4921 caattgtatc tgcgttggtt tctgttacgt agtttcttat atttgcatct gtaactttta
     4981 atttgttgtt tattctatgt tctgttgtgg attttgtaat gtcctttgat ttttcctgta
     5041 actctcgttc ttgtgttcct gatttagttc caatgagaga tataagatta cttagggagt
     5101 ttgttacttt ttttgaagct tcaaatatcg tttcccgtgt taaatatgtg tctccatttc
     5161 ttgataaaaa atcagtctga agtacttttg aatcagtgtt tgagttgaat ttattgattt
     5221 gcgaagctac tctatgagaa agagaagcgc tacttttagt tgtatctgat gagttatcat
     5281 tactgcttgt cactccttcc gggtggagag ggggggtggt tgaagagttc tgtatgttca
     5341 gaggcctgtg tccccgagaa agagagttat ttgcagatct ttcttgacta ctcgtagtta
     5401 acaggtctga acctattcca tccgaattat ctggagtatg gttgaaagcc ttttctaaag
     5461 ctgaattatt tgcaaatgtc tggacagtat ctgatataga gtgtggtaga acggaagtta
     5521 ctttttgaac tagtgctctt tgtggattta gtaatgagtt tccgttacgg gataatagat
     5581 cgtttgtctg agaagtagga gactgtgcat cattttctgg gttctcgtga tagctattgt
     5641 catatactct gttgtcaaaa actctattat cgtaagttct attatcatta tttatatgat
     5701 agtgaattac aggttgggat gttttctcat tggctccggg ggttggtccg ccatctggca
     5761 tatcttcggg aggtgtaggg aggccatctt ggataggatg cgaatctttt atatcttcta
     5821 aaatatcatt aaggactttt ctcaacagct caggaaacac ggctactgta tttaagtctc
     5881 ttgacagttt acctatacca aatcctttac tatcaatagt atttttacgt tgattgcgca
     5941 aactatcaaa ggcaagtttt gcctgtgcgt caactaaatc atctagtttg agagatgaaa
     6001 cagtttcatt acttaacttt aattcagcaa gaatattatt cttgataaat gatgatactt
     6061 cagtattgag gagtttttgt cccaatgata aagcttcacc agatgtgtgt tgttgtgcta
     6121 atagtgcaag tctgtgctca atgttttgtc gtaattgatg gagtttctta ttctttgccg
     6181 accatggtcc aaaggcagaa tcttgggata atttatcttt tataattagt tccgaatact
     6241 gtgcgacaat tttttgaagt cccttttcgt tttcaaaatt aagaccattt tgatgtacta
     6301 aggagtacaa aacatttgaa aattcgagaa tctcgctttt ggcatacgca ttcattgagt
     6361 tggaataaat gcttcttaac acatcttctt tccctgagga agaaaataaa aaatcaagac
     6421 atttctgatt aaaagacgtt gcaaagtttt ctttttcaga cactgatgct ggtgttttta
     6481 gagaagtttg cgaactatgg atatcggata ttttgctttt taactcgctg tattctgttg
     6541 atgatggtga agttgcctta tataagaatg ttggcgcttg agtattattt acattatgca
     6601 tggcgcacct cagaaatgga gaaaaagttt atctgtatct gtacatgagc ttattgtact
     6661 actcaaaacc tttactaaat tatcaaaaat actattggca ttactgtatt tttgaactaa
     6721 agtttgaaga ttatttttca ttgtttcatc ttcggcagag aatccggcat tccatgcctg
     6781 atattttgca ttatctagca caacctcgcc atttccacct agattatcta agctttttaa
     6841 catattgtct attggggtca tgtttatact gacaacatat cccccgtttt tttgagatac
     6901 cttgccgatt gttccaccta attctgtaag ccatttattt gcttgttcct gactaacagt
     6961 attatttgct ggatatagcg gtttatcttt atatttttcc ttgagttctt ccaatgcctt
     7021 tttaagcgag ttgacttgta atttcacgga gtttccgtcg ttacctccgg gagagatcca
     7081 gccggcaaga ctggaaagaa cagcgctaaa atcttgatac atttgagtat atgaactaac
     7141 ggcatgttca tatactttca gatactgttc attaatatca ttgatggagt ttgcaatttt
     7201 agcccacagt tctctatgag atatcatttg atctccatca agctcggctt ctttcggagc
     7261 tgaatgtaat aattctcttg cgtctttatt aattggatat tcgtgcctgg aaagaatatc
     7321 taatagttga gcggatttat ttacatccat gctaatctga gatgaatgta atgctatttc
     7381 ttctagcgat gttttagtca acgttttttg tgaaagctct ttctttaatg cctgatttgt
     7441 tgttctgata ttatgaaggg tgtcgttgag catagtaagg gaacttacag gatgagaact
     7501 ggtcgttgtt tttatatcag aattaactgt ttcggttgat gaaccgttgg tattgtttgg
     7561 actgaatgat gaggtggaaa tactattagt cagagttgtt atattcatgg ttatttcctt
     7621 acaggaagag ccatatatta ttatggctct tggatatatt aagctcgaat gttaccagca
     7681 atctgactgg ctgtcgaatt ctttgattgg ttgatgctgt caattatatt caataatttt
     7741 tgtattaatt gatttgtcgc ttgggatgct tctttagata cttgggatgc ttcctctgct
     7801 tgtttactgc tggcctgact gattagttgt tcttcttctt caagagcaga tgcataacgc
     7861 cctcctgatg tggatatatt accagcaacg gctgatgttt ccattgttgc acggccaatg
     7921 ttttgttttt gctgcgcaga aagggtattg agctcgtaag ttcttctctg ggtgtcaatt
     7981 ttatcctgca aagaaatatc gggagaacta agagaagttt tatgttcagt tgacagggat
     8041 atattatctg gcgccagttt atttttacct aaaaattttg tgctagagtt agtttgtggt
     8101 gaggtgatat ttgtatctat ttgtttattt aaccctaatt tagaacctgc aagctctttt
     8161 tcaagagatt gagcagtggc aaggttcttt cttaaggctc ctttttggtc gctaatccct
     8221 gaatgcgttt ttttggcacc gatacccgtt atacctactt gtgtgactgc tccagtaatg
     8281 cttgatgata gggctgccag gccttgccga acaatgttct ctgcagctga ttttgtagca
     8341 tcgaacgcaa tcaatgacaa ttgagagccc aatttagttt ctgcagtgcg gagagcagaa
     8401 agtagaacgg ctacactaat aattaaagaa acagcattag aagaaagact ggaaatatcc
     8461 agagtgttct ctggagtaag ggtgtgactc atctctgaaa gatctttcat ctttttgtca
     8521 gtaaggcgag caatgatttc tgaatcatgc tgaacctgct ctgataactt taaaagttga
     8581 tcatcattta atgtggttgt taatacggga tttttaccga cattaagtgg aatatgcgct
     8641 gcaatctgct gataattttg tgatttttgt gtttcggaag aactttgagt ttgttttgtg
     8701 gatatatctg tatataaaat ctgggttggt tttgtgtttt gaatttccat aacattctcc
     8761 ttatttgtat caagcagtag tttgttgcaa aattgctttt gcaacatcag ttctattagc
     8821 ctgagagttg gacattgagg ctaatagatc tgcaattact tcctgcaatt ggccgaattt
     8881 ttctattgct tcactgatat attttgacag ttgttcaact tgatatttcg ataatgtcag
     8941 gtctgctaga tttgtcgacg cgctgttctg gaaaacagca gaagcgacac ttcctcccgc
     9001 ttgtgtggca gagttaacac tttctcctaa taaaactgct tggtttaaat gggtggaaat
     9061 aatttgtttg gatattactt catcacccgc tgcaccaaga aatttattta atctggcaac
     9121 agcattagtg attaaatcgt ccagttgaga agaaaaattc ttgagaaact ttggtataag
     9181 gtctgtgagg gttttaccta ttattttgcc aatattttct gcaagttttg ctgctgcctg
     9241 tttaccaaca gtggctacga gaacgactgc tgcaactagg acaagagcgc ctgcgattgc
     9301 ccccagaata gagccaatca ttttggcttt tttcgagtcg acgcccaagc cttcgagcat
     9361 ttttgtaaat gcatctgaaa ggagtttgat taagggttca atgactgctt tcatgatcgg
     9421 attcagggct tgttccatga aggaattgcc ggtcgctgct tgtactatag catccgtaac
     9481 cataagagct aaaccaacag ctgccagtgc tagagaggct cctccagaaa aagctgctgc
     9541 aacaacacta acgatagtta aaagtgcccc aagtattttc ccaacacaac ccattactct
     9601 gttgagttct tctgctttac gtacttcagc agcatactca tcagattttc tctccatttc
     9661 agtttttctt gattcttgga gagactggaa tagagccaga tcatttttta aagattcttc
     9721 attatttttt ccaactagtt gaataaaggt tgccatcaat tgagtaacac tggcaagtcc
     9781 ggttaatgat ttctgctggg ttgatagctg ttcagcagat gctgtgtttg aaaatgcaga
     9841 aaaagagtct atttcttttt cgagttgcat tgatttatct gtaagtttgc tatgaattga
     9901 cagggttttc tgctcaatca atgtcctgtc tttaactgct gcgtcttttt tgatagtgag
     9961 ttgtatttct tcccggctta attttttctt ttctggtgac tctgggtcga gttcggataa
    10021 tcttgtttga atttggttaa ttttattttc taggtctttt attttagaat ctgcgttttt
    10081 tagtttatta atttgttttt catagtctct ggttagtcct tcagtttcag atagaagagt
    10141 gttaatttta tcggagaatt ctaggttttt ttgctgtctt gcctgttgct gggacttcca
    10201 agcagtaatt ttatttgtta atgcagttaa agatttttca ccgagtattt gaataaggtt
    10261 tccaattaaa agcgttagtt gggaacttgc atttaatgac tttggtgctt taagttcagg
    10321 gattaatata tttgaagttg agtgtgcatt agttgtatta atattttggt tagcagtaag
    10381 atcagcaatt gtaagagaaa ataatttgtt agctgcatca tttgcagctt ggatagtatt
    10441 gtctccaagc tcagtggaag ccaatatttt ggcaagagga aaaccagtgg ttgtggtgct
    10501 tacattatgc ataataatta ctccttgata tcctgaattg cgtccaagta tgattgtgct
    10561 tttattttta atttttcatc attgctgtgt tgaattacga gttcgaagca ctctttagct
    10621 tttaaggggg ctttcaaccg aagctggcat tgtccagtat ggaatactgg tgtatagtca
    10681 ttttttccta atgcaaaagc gacagcataa aggtctgctg cttgttggaa ctgttctttt
    10741 atctgataaa tagctgcgag tcccataatg tagtctacat tgtaaaagtc gtatatacat
    10801 aaaaacctga agaaaacttc agcttcctct attcttcctt tgttgtaaaa gtcataagca
    10861 tatgaataaa tgtcatccat catatcatca ggaattgcat taatatcttt cagtgtagcg
    10921 ccagagttaa ttgcatcaat tactgcagta gagatgcttt cattttcggt gatatttaaa
    10981 gacataaggt ctcctttttg tctgctttac tttaataagt ataagattta atataaaaga
    11041 tttaatttgt attgctttga cggtatacag cctttagaac ctctgcgctt atttctattt
    11101 gtaaaacttt tgttgattca ttaaatattt tatcgtagac ggaactggat actagatttg
    11161 taagatcctt catttgtgtg ttaaacaaat ccatgtcact gctatagcgt tttaaaatct
    11221 ttgctctgag gccagatgag gcaatctcgt ttagtgctgt atgactgata gatgtttgtg
    11281 ctgatttttc atcaagtttt atatccggga attttttttc aatagctgca aatatgtttt
    11341 ttttgttatc tgaggtcaaa acaactccat tgtgtatggc catttcatct atacttctgt
    11401 ttatcattga agagacataa gtggttcgct cttggctcat ccaacaaaat agttgattcc
    11461 ccgaattact atcttttatt ttgtctatga cgctgtccct gattttattt atattcaccg
    11521 ggcatccaaa attcttctga ggaaagcgtt gttttaagtt aaaaacagat aatattttct
    11581 ttacaagctt attgcgactt aacgaatcaa atgatgggcg aggaatcgcg aattcaacct
    11641 gtggaagtat tttgtttaga atttgcatga taccccctat atgttagttc acttctgaag
    11701 tgatgtttgc aatggttagt ttaatacaaa ataaaaattc atacaaagat tcaatgagtt
    11761 catcaggtga aagatatttt ttgtagtgtt tcttggtcaa aacacattga tgagtatcag
    11821 ggagaagaca gatagaagaa ccataatctc tactcaaaga gtagttaagg atgagtatgt
    11881 gttctggatt aataatattt tctgtaaaaa gttcatgttc attaagaagt ccggtcaaaa
    11941 agatgtattc atcattatct gttatacaaa caggtatttg ttcttcgagc aatataaagg
    12001 cttgattttt atcgttaaaa tcaagctttg cacctgttat ttcataaagt ttatcatata
    12061 gtttgcgaca catttctatg gccttctata tattagaatg agagttattc aataaaagct
    12121 tattgatctg taataagtcg tgaatgtaaa gtttggttcc aagatctggc gatttaagag
    12181 aattgtaata atcaataatt tttattgatt tttcgatgat ttcttctttt ttatctgttg
    12241 cttcggtttt taattcgttt aatacacgag tcatattgct gggtgctgat tttactaagt
    12301 atgagtggca agcgttgatg accgcatcaa agtcttctga agatacattt tcagtaggat
    12361 gtttgtaaag tgaaattgat aaaggcttga atgtttttgg tgtgtggata aggttaattt
    12421 tattgaaact tttaagatag ctgcgtcgta attgagtcaa tctggaaaga tcaaaatcag
    12481 attcaatata cttcattatt tcgttataag actgattgag actttcaatg cgttgcctaa
    12541 ctttatttgc atacgcgtat acatcgttag gtgtagcaac caatttaacg tcagggagtg
    12601 gaaagtatat ttcagaattt cctgctttaa gaacattaag tgccatacca gcacagtttt
    12661 cagatttact gattaattta taatttgcca attgtctgaa agcatctgct ttctttttgc
    12721 atataaattt tgacatgttt gtttcatcaa ggccaaaaag gttatggctg attttaccat
    12781 cctttgtttt attatcacct gataatggaa tgtatacttt atcagcactg accccccaat
    12841 aaccataggg gtcttttttt tgattagctc tgggaaaaaa tatttttttg ttgttatttt
    12901 gatcttgttt tgaatttaat aagtttctaa cctcttggcc tgcatcaagt ctttcggctg
    12961 tttggtctga aatcatattt agtttgtctt gcttataggt atcgacactg taacctgagc
    13021 gtttttcaaa aaggtagttt atttctgtat cttttgtaac attcccgccg ggataccagc
    13081 tgagataggt atgagttggt tcctgtagta aattgttttt tatactgata ctagtatgcc
    13141 ctaaatgggt tatgcctcta tttccccagc aacgaaaaac taaatgtctt tcaagatttt
    13201 tggctgtacc gatgccatga aaacgctctg cggtattcaa cattaatttg atgtctctac
    13261 ctgtgaaaga gtgttgaatt gagtttttag ctgcttcata ggcggcaaac gcactttctg
    13321 cagatttttc gctagctaat gtattgagga aaagcaataa ggatttttga ttctctagtc
    13381 gataattttt tagttgttca tttaatatct gtgaatttat ctttgcatta atagtgctta
    13441 atgttctgaa aaagagatcc tttaggttga atttctgtgc tatcaatata ggtccatgct
    13501 ctgtatcttc aacgcgtata ggcccttttg tattgcttgc gtcaatgaaa ttgctaattt
    13561 tgaggatcat actttattaa ctctccatta cttggtgatt gtggggatgt aaaatgtatt
    13621 tggtatataa tatattcaat taataaattt agaaacttga gcctgttaac ataatcaaat
    13681 tttcttttgc tgtacataat atgtacctcg tgagcatatg tagtgctcgt ttcatcatga
    13741 aatcccacaa gataaagtgc ctgatgtatc aggctcggag tgttatagaa aaagagagaa
    13801 ccctgttgaa taagtatctt ttttgttgta aaacgaggta caaaactgca atcaatgccc
    13861 catttatgac tgaggataat taaatgcaca taactaattt gggattgcat caggtttcat
    13921 ttcaaagcgg agattcctat aaaggcgcag aagaaaccgg aaagcacaaa ggtgtaagcg
    13981 tgatttcata tcaaagagtt aaaaatggag aaagaaataa ggggatcgaa gcgcttaatc
    14041 gattatattt acagaaccaa acaagcttaa caggaaaaag ccttttgttt gcgcgtgaca
    14101 gagctgaagt tttttgcgag gcaataaagt tggcaggtgg tgatacgtca aaaattaaag
    14161 ccatgatgga acgattagat acctataaac ttggtgaagt taataaaaga catattaatg
    14221 agcttaataa agtaataagt gaagagataa gagcacagct aggcattaaa aataaaaagg
    14281 aattgcaaac gaaaataaaa caaatattta ctgattattt aaataataag aattggggac
    14341 cggtcgacaa gaatattagt catcatggga aaaattatgg ttttcaatta actcctgcct
    14401 ctcatatgaa gatagggaat aaaaatatat ttgtcaaaga gtataatgga aaaggaattt
    14461 gttgtgcttc tacgagagag agtgatcata tcgcgaatat gtggctttca aaagtggtgg
    14521 atgatgaggg gaaggaaatt ttttcgggta tcagacatgg agttatatca gcctatggat
    14581 tgaaaaaaaa ctcatctgaa agagctgttg ctgctcgtaa taaagctgag gagttagtaa
    14641 gcgcagcatt atatagcaga ccagagttat tatcacaggc tttgtctggt aaaacagtag
    14701 atttaaagat tgtttcgact tctcttctga cgccgaccag tttaaccggg ggagaggaga
    14761 gtatgttaaa agatcaggtg aacgcattaa aagggttaaa ctctaaaagg gggaagccaa
    14821 cgaaattatt aattcggaat agtgatggtc ttctgaaaga agtaagcgtt aatctaaaag
    14881 tggtgacatt taactttggt gtaaatgaat tagcgctaaa aatgggctta ggctggagga
    14941 atgttgacaa gcttaatgat gaatcaatat gttctttgtt gggggataat ttcctcaaaa
    15001 atggtgtgat tggaggttgg gctgcagaag caatagaaaa aaatccacca tgtaagaatg
    15061 atgtgatata tctggctaac cagataaaag agattgtaac caaaaagtta caaaaaaatg
    15121 ataatggaga gccgtataaa ttgtcacaaa gaatgactct attggcttat actataggtg
    15181 ctgtaccttg ctggaattgt aagagtggga aggacagaac aggtatgcag gatgctgaaa
    15241 ttaaaagaga aataataaga aaacatgaaa caggtcagtt ttctcaatta aatagtaaat
    15301 tatcctcaga agagaaaaga ttattttcta ctattctaat gaatagtggt aatatggaaa
    15361 tccaagagat gaatactggt gtgcccggaa ataaagtcat gaaaaaattg ccgctatctt
    15421 cgttagagct atcttattct gaaagaatag gggacccaaa aatatggaat atggtgaaag
    15481 ggtattcgtc atttgtataa gaggaatata tggaagattt agcagatgtt atttgccgag
    15541 ccttgggtat ccctttaatt gatatcgatg atcaagcaat catgcttgat gatgatgtgc
    15601 taatatatat tgaaaaagaa ggagattcaa taaatttatt atgccctttt tgtgcattgc
    15661 ctgaaaatat aaatgatctt atatatgcgt tgagcctaaa ttactcagaa aagatatgct
    15721 tagctactga tgatgaaggc ggaaatctaa ttgcgcgatt agatcttacc gggataaatg
    15781 aattcgagga tgtatatgtt aatacagaat attatatttc gcgtgtgcgt tggctgaagg
    15841 atgaatttgc gcgaagaatg aaggggtatt aatgtcccgt ttcgtattta ttttgctgtg
    15901 ttttatccca tatttaggga gagctgattg ttgggataag gctggtgaaa ggtacaatat
    15961 tccatcaagt ttgttaaaag cgattgcgga aaaagaatcc ggatttaata aatctgcagt
    16021 caatgttaat aacaatggaa gtaaagatta tggtataatg caaataaatg actttcattc
    16081 taaaagactt agagaaatgg gatattctga ggaaatgcta attagtcatc catgtctttc
    16141 tgtacattat gcagcaaagt tattaaatga gtttatgatg atgtatggaa gagggtggga
    16201 ggctgttgga gcctataatg caggaacgtc tccgaaaaag aaaaaagaaa gattaaagta
    16261 cgcagaggat atctatagac gctatttgcg gatagcagca gagagcaagc agaataatcg
    16321 aaggatataa gaggatttat gtctgaggca aagaactcaa atcttgcacc attcaggtta
    16381 ttagtgaaac tcacaaacgg tgtcggggat gagtttccgt tatattatgg gaataactta
    16441 attgttctcg gacggacgat agaaacactt gagtttggaa acgacaattt cccagaaaat
    16501 attattcctg taacagatag taaatcggat ggtataattt atttgaccat aagcaaagat
    16561 aatatctgtc aatttagtga tgaaaaaggt gagcaaattg atatcaattc acaatttaat
    16621 tcatttgaat atgacgggat ttcttttcac cttaaaaata tgagggagga taaatcgaga
    16681 ggccatatac taaacggaat gtataagaac cactcggtgt tttttttctt tgctgttatt
    16741 gttgtgttaa ttataatttt tttcctctct ttaaagaaag atgaagtaaa agaaattgct
    16801 gaaattatag atgataagag atatggaata gtcaacactg gacagtgcaa ttatattctt
    16861 gccgaaacac aaaatgatgc ggtatgggcg tctgtagcct taaataaaac cggatttacc
    16921 aaatgcagat atattctggt gtcaaacaaa gaaataaata gaattcaaca atatatcaat
    16981 caacgattcc catttatcaa tttgtatgtg ttgaatttag tgagtgataa agcggaatta
    17041 ctggtgtttt taagtaaaga aagaaatagt tcaaaagata ccgaacttga taaattgaaa
    17101 aatgctctga ttgttgaatt tccgtatatc aaaaatatta aattcaacta tcttagcgat
    17161 cataatgcaa ggggggatgc taaaggtatt ttcacaaagg taaatgtcca gtataaagag
    17221 atttgtgaaa ataataaagt tacatattct gtcagagaag aactgacaga tgaaaagctt
    17281 gagctcatta atagactgat ttctgaacat aaaaatatat atggagatca gtatattgag
    17341 ttttctgtac ttttgataga tgatgatttt aaaggtaaat catatcttaa cagcaaagac
    17401 agttatgtaa tgttgaatga taaacactgg ttttttttag ataaaaataa gtgaggataa
    17461 aatgagtgtt acagtaccga atgatgattg gacattgagt tcattatctg aaacttttga
    17521 tgatggaact caaacattac aaggtgaact aacattggca ctagataaat tagctaaaaa
    17581 tccttcgaat ccacagttgc tggctgaata ccaaagtaaa ttatctgaat atacattata
    17641 taggaacgcg caatccaata cagtgaaagt gattaaggat gttgatgctg caattattca
    17701 aaacttcaga taatagggag cattcatgaa ttacatttat ccagtcaatc aggttgatat
    17761 tatcaaagcc agtgattttc aatctcaaga gatatcaagt ctggaagacg tcgtgtcggc
    17821 taaatatagt gatattaaga tggatacaga tattcaagta tcacaaataa tggagatggt
    17881 aagcaatcca gaatcattaa acccagaatc tttggccaag ttacagacga cgctctcaaa
    17941 ttattcaata ggagtatcat tagctggcac gttagcaaga aaaacagttt cggctgttga
    18001 aactttatta aagtcttaat ttatatgatt aggtataaag gttttatttt attcttgttg
    18061 ctgatgttga ttggatgtga gcaacgtgaa gagttaattt ctaatttatc tcaaagacag
    18121 gcaaatgaaa taatatctgt gctagaacgc cataatatta ctgctagaaa agttgatgga
    18181 ggtaaacagg ggatctcggt acaagtcgaa aaggggacat ttgcatcggc agttgatttg
    18241 atgcgcatgt acgatttgcc aaatccggag agagttgata tctcacaaat gtttcctaca
    18301 gattcattag tgtcttctcc aagagctgaa aaggcccgtt tatatagtgc tattgagcaa
    18361 cggctggaac agtctttagt ttctattggt ggtgttattt cggcaaaaat acatgttagc
    18421 tatgatcttg aagaaaaaaa tatatcttca aaaccgatgc atatatcagt aatcgctata
    18481 tatgactcac cgaaagagtc tgaactatta gttagtaata ttaagcgatt tttgaaaaac
    18541 accttttctg atgttaagta tgaaaatata tctgtcatat taactccgaa agaagaatat
    18601 gtttatacaa atgtacaacc tgttaaggaa gttaaatcgg aatttttaac aaatgaagta
    18661 atatatttat ttctcgggat ggctgtacta gttgtcattc ttttggtatg ggcattcaaa
    18721 acagggtggt tcaagagaaa caaaatatga taagaatgga tggaatttat aaaaaatatc
    18781 tttcaataat ttttgatcca gcgttctata taaatagaaa tcggttgaat ttgccttctg
    18841 aactgttaga aaatggcgta atcagaagtg agattaataa tctcataatt aataaatatg
    18901 atctaaattg cgatattgaa cctttaagcg gggtaaccgc tatgtttgtt gccaactgga
    18961 atttacttcc agctgttgct tattttatcg gtagtcaaga gtctcggtta attaatcata
    19021 gtgagatggt tattagttat tatgggggca aaatcagtaa gcaaggggag gccgctattc
    19081 gaagtggttt ttggcatctt attgcgtgga aggaaaatat atctgttggt atatatgaaa
    19141 gaataaactt attatttaat ccaattgctt tggagggaaa ttatacgcct gtagaaagaa
    19201 atttgtcccg attaaatgaa ggtatgcaat atgcaaaaag gcactttacc ggtatccaga
    19261 catcatgcct atgatggtgt tgttataaag agaattgaaa aggaattatg taaaaccata
    19321 aaagatagag acactgagag taagaagaaa gcaatttgtg ttataaagga ggctacgaaa
    19381 aaagctgaat cactgcgtat agatgctgta tgtgatggtt atcaaattgg tatacagact
    19441 gcttttgagc atattattga ctatatttgt gaatggaagt taaagcaaaa tgaaaataga
    19501 agaaatattg aggactatat aactagtttg ctttcagaaa atttacatga tgagaggatc
    19561 attagcactt tattggagca gtggcttagt tcgttgcgta atacagtaac agaattaaaa
    19621 gtggttttgc ccaagtgtaa tttagcactg aggaaaaagt tagaacttga tctccataaa
    19681 tatcgctcag atgtcaagat tattttaaaa tattctgaag ggaataatta tatattctgt
    19741 agtggtaatc aagtagtgga gtttagtcct caggatgtaa tatcaggtgt taaaatagaa
    19801 cttgcggaaa aattaacaaa aaatgataaa aaatatttca aagagttagc acataagaaa
    19861 ctgagacaaa ttgcagaaga cttgttaaaa gagaatcctg ttaatgatta atcaaataaa
    19921 tgcaagcaat gctctgcagc aaagattaaa tagtgaagaa gttgttaatt taaatgagag
    19981 attatcatct tcgcaatcat ttgatgagga tataatctat gaaataatgc aatatttctc
    20041 acagagtgaa ttaaattcca tagataatga tgaactgcac aataagattg aacaattatt
    20101 caattcacgt tttccttatc ttacagctgc acaaaaaagt tcacttctta ataaactcat
    20161 agatgcaaat caatatgtcg atttgcatga gggattttat gcatcattgt ccatatataa
    20221 caatattgat ttctatatta agactacaac cttcgattca ttaattagtg tctttgaagc
    20281 agggagagag gcagatgatt cgacatggta gtaataagtt gaaaatattt attttaagta
    20341 tattgctatt aacactgagt gggtgtgctt taaagtcatc atctaattct gaaaaagaat
    20401 ggcatattgt tcctgtaagt aaggattatt tttctattcc aaatgattta ttatggtcgt
    20461 ttaatacaac caataaaagt ataaatgttt actctaaatg tattagtggt aaggcggttt
    20521 atagttttaa tgcaggtaaa ttcatgggca actttaatgt taaggaagta gatgggtgct
    20581 tcatggatgc acaaaagata gctatagata aactattttc tatgctgaaa gacggggttg
    20641 ttttaaaagg taataagata aatgatacca tccttataga gaaggatggg gaagttaaat
    20701 taaaattaat tcgagggata taattgtatt gtgagtaaat ataaaggtct aaatacaagt
    20761 aatatgtttt acatttactc tagtggacat gaaccagtta acgttgagct tgtaaaagat
    20821 aaagaacgta acataattga gctggctcca gcatggaagg gttttttttt gtccgaaatc
    20881 aaaatataaa attcagtgat aatgtgaact atcattatcg ctttaacatt aatagttgtg
    20941 caaaatttct tgctttttgg gactatttca gtggtgctct agtagagcat agccatgctg
    21001 aaaaatgtat tcatttctat catgagaatg atcttcggga tagttgtaat accgaatcga
    21061 tgcttgataa gttaatgtta agattcatct tttcatctga ccagaatgta agtaatgcac
    21121 tggctatgat acgaatgact gagtcatatc atttggttct ttatcttctg agaactattg
    21181 agaaagaaaa ggaggtaaga ataaagagtc tgacagagca ttatggtgtg tcggaggctt
    21241 attttagatc tttatgtaga aaagctttag gggcaaaagt caaagaacaa ctgaacactt
    21301 ggcggttagt aaatggttta ttagatgtat ttttgcacaa tcaaacaatc acttcagctg
    21361 ccatgaataa tgggtatgca tcaacatccc atttttcaaa tgagataaaa acacgactag
    21421 gcttttctgc aagagaatta tcgaatatta cttttttagt gaaaaaaata aatgaaaaaa
    21481 tttaatatta aatctttgac tctcttgatt gtattgttac cattgatagt taatgctaat
    21541 aatattgact ctcatttatt agaacaaaat gacatagcga aatatgttgc tcaaagtgat
    21601 acggtaggat ctttttttga acgtttttct gcgttattaa attatcctat tgttgttagc
    21661 aaacaagctg caaaaaagag aatatctggg gagtttgatt tatctaatcc agaagaaatg
    21721 cttgaaaaac tgacattact tgtgggactc atatggtata aagatggaaa tgcattatac
    21781 atatatgact ctggagaatt aataagcaaa gttattttgc tggagaatat atcacttaat
    21841 tatcttattc agtatctgaa ggatgctaac ctttatgatc acagatatcc tataagagga
    21901 aacattagtg ataaaacatt ctatatatct ggtcctccgg cattggttga actggtagct
    21961 aatacagcaa ctttgcttga taagcaggtt tcttctattg ggacagataa agttaatttt
    22021 ggggttataa aattaaaaaa tacatttgtt agtgacagaa cttataatat gagaggggag
    22081 gatattgtca tcccaggggt ggctactgtt gtagaacgtt tattaaataa tggtaaggct
    22141 ctgagtaaca gacaagccca aaatgatccg atgccaccct ttaatattac acagaaagta
    22201 tctgaggata gtaatgactt ttcatttagt tcagttacca atagttctat attagaggat
    22261 gtatcactta ttgcatatcc agaaaccaat tctatattag tcaaaggtaa tgatcagcaa
    22321 atacagatta ttcgcgatat tatcactcag ttagatatag ccaaacgaca tatagaatta
    22381 tcattatgga taatcgatat tgataaatct gaattaaata accttggtgt taattggcaa
    22441 ggaacggctt catttggtga ttcgtttggt gcgtcattta atatgagctc atcagcatcg
    22501 ataagcactt tggacggcaa taagtttatt gcttcagtta tggcattaaa tcagaaaaag
    22561 aaagcaaatg tagtttcaag gccggtgatt ttgacacaag agaatattcc ggctatattt
    22621 gataataaca ggacttttta tgtgtcatta gttggtgagc gcaattcttc gttggagcat
    22681 gttacgtatg gaacattgat aaatgttata cctcgctttt catctcgggg acaaattgag
    22741 atgtctttaa cgatagagga tggaacagga aatagtcagt caaattacaa ttataataat
    22801 gaaaatacat ctgtccttcc agaagttgga aggaccaaaa tcagtacaat agcgagagtt
    22861 ccacaaggca aaagtttgtt gataggtggt tatacgcatg aaacgaactc taacgaaata
    22921 atatctattc cttttctctc ctctattcct gtaatcggta atgtgttcaa gtataaaaca
    22981 agtaatatca gtaatattgt aagggttttt ctgattcaac cccgtgagat aaaggagtca
    23041 agttattata atactgcaga atataaaagc ctcatatctg aaagagaaat acaaaagact
    23101 acacaaataa tcccatctga aactactttg ctggaagacg aaaaatcatt ggtttcatac
    23161 ttaaattact aactataaag taggtgatgt atgcttgatg ttaaaaatac aggagttttt
    23221 agctctgcat tcattgataa gttgaatgca atgacaaatt cagatgatgg agatgagact
    23281 gctgatgcag agcttgattc tggcttggct aatagcaagt atattgactc atctgatgag
    23341 atggcttccg ctctttcgtc atttataaac agaagagacc ttgagaaact gaaaggaaca
    23401 aatagtgata gtcaggaacg tattttagat ggggaagaag atgaaattaa tcacaagatt
    23461 tttgatttaa agagaacgtt aaaagataac cttcctctag atcgggattt tatagacaga
    23521 ctaaagagat attttaaaga tccaagtgat caagtcttag cattaaggga acttttgaat
    23581 gaaaaagatc ttactgctga acaagtcgaa ttattaacta aaattattaa tgagataata
    23641 tcaggtagtg aaaaaagtgt taatgctgga ataaattcag ctatacaggc taaattattt
    23701 ggcaacaaaa tgaaacttga accacagctt ttgcgtgcat gttatcgtgg ttttatcatg
    23761 gggaacatat caacaacaga tcagtatata gaatggcttg gtaattttgg ttttaatcac
    23821 agacatacaa ttgtgaattt tgtagagcag tcactgattg tagacatgga ttctgagaaa
    23881 ccgagctgta atgcttatga gtttggtttt gtgttatcta aattaattgc aattaagatg
    23941 attagaactt cagacgtaat ttttatgaag aaactggaat cctcaagctt gctaaaagat
    24001 ggcagtttaa gtgcagagca gctattgcta actttattat atatttttca atatccaagt
    24061 gaaagtgagc aaattcttac ttctgttata gaagtatcac gagccagtca tgaggattct
    24121 gtagtgtatc aaacatatct atcttctgtt aatgaaagtc ctcatgatat atttaaaagt
    24181 gaaagtgaaa gagaaattgc gatcaatatt ctacgagagc ttgtcacaag tgcatacaag
    24241 aaagagcttt ctagataaca ggagataaaa gtgatccagt cttttcttaa gcaagtaagt
    24301 actaagcctg aattaattat attagtgctt atggttatga tcattgctat gttgatcata
    24361 ccattaccta cataccttgt tgatttttta atagggctta atattgtact tgctattctc
    24421 gtttttatgg gatcatttta tattgaaaga atattgagtt tttctacatt cccttcggtt
    24481 ttgttgataa cgacattatt tcgtctcgct ttatcaataa gtactagtcg actaatttta
    24541 gttgatgcgg atgcgggtaa aattattact acatttggcc agtttgtcat tggtgatagt
    24601 ctggctgtag gttttgttat tttttcaata gtaactgttg tgcagttcat tgttataaca
    24661 aaaggatctg aaagggttgc ggaggtagct gctcgctttt ctcttgacgg tatgccgggg
    24721 aaacaaatga gcattgatgc ggatttaaag gctggaatta ttgatgccgc aggagctaaa
    24781 gaaagacgca gtattctgga acgtgagagt caattgtatg gttcatttga tggagcaatg
    24841 aagttcatta aaggtgatgc aattgctggc atcattatta tttttgtcaa tttaatagga
    24901 ggtatttctg ttggtatgag tcaacatgga atgtccctat ctggtgcatt atctacttat
    24961 accatactaa ctattggtga cggattggtc tctcagattc ctgctctgtt aatttctata
    25021 agtgcaggat ttatcgttac aagagttaat ggtgatagcg ataatatggg acgtaacata
    25081 atgtcccaga tatttgggaa tccttttgtt ctcattgtta catcagctct tgccttggcg
    25141 atagggatgt tgccaggttt tccgtttttt gtttttttcc tgatagcagt tactttgacg
    25201 gctttattct attataaaaa ggtcgtagaa aaagaaaaaa gtctgtctga gtctgattct
    25261 agtggctata ctggtacatt tgatattgat aatagccatg actcatcttt ggcaatgata
    25321 gaaaatctgg atgctattag ttcagaaacc gttcctttaa ttttattatt tgccgaaaat
    25381 aagataaatg caaatgatat ggaaggtctt attgaaagga taagaagtca gttctttatt
    25441 gattatggtg tcaggcttcc aactatttta tatagaacaa gcaatgagct taaggtcgat
    25501 gatattgttt tgttaataaa tgaggtgcgt gccgatagtt ttaatatata ttttgacaaa
    25561 gtgtgtatta cagatgaaaa tggagatata gatgctctag gtattcctgt tgtttcaact
    25621 tcatataatg aacgtgttat ttcttgggta gacgtttcat atacagaaaa tctaactaat
    25681 attgatgcta aaattaaaag tgctcaagat gaattttatc accagttgtc acaagcttta
    25741 ttaaacaaca taaatgagat ttttggtata caagaaacaa aaaatatgtt agatcagttt
    25801 gaaaatcggt atcctgatct attaaaggaa gtcttccgac atgtgactat acagagaatt
    25861 tctgaggtat tacaaagatt gcttggagaa aatatttctg ttcgcaattt aaaacttatt
    25921 atggagtctt tggcgctttg ggctcccaga gaaaaagatg tcataacatt agttgaacat
    25981 gtccgtgcat cactttctag gtatatttgt agtaaaatag ctgtttctgg tgagattaaa
    26041 gttgtgatgc tttccggata tattgaggat gcaataagaa aagggataag gcaaacctct
    26101 ggtggctctt tcttgaatat ggatatagag gtttcggatg aggtaatgga aactttagca
    26161 catgctttga gagaattgag aaatgcaaaa aaaaatttcg ttcttttggt atcagtagat
    26221 atacgtaggt ttgttaaaag acttatagat aacagattta agagtatact cgttatatcg
    26281 tatgctgaga ttgatgaagc atataccatt aatgtattaa agactattta gtgaggttta
    26341 aatatgagta acattaattt agttcaatta gttagagata gtcttttcac gattggttgt
    26401 cctccatcaa ttattacaga tttagacagc cattcagcga ttaccatttc tttggatagt
    26461 atgcctgcta ttaatatagc tcttgtcaat gagcaggtta tgttatgggc taattttgat
    26521 gcaccaagtg atgtaaaact tcagtcatct gcttataaca tattaaattt aatgttaatg
    26581 aactttagtt atagcattaa tgagctggta gagcttcatc gttctgatga gtacttacag
    26641 ttaagagttg ttattaaaga tgactacgtt cacgatggga ttgtttttgc agagattctg
    26701 catgagtttt atcagaggat ggaaatctta aatggggtct tataatcaat gagctataca
    26761 aaattgctca ctcaattatc ttttcctaat agaatctcgg ggccaatctt ggaaacaagt
    26821 cttagcgatg tttcgattgg tgagatttgt aacattcagg ctggaattga aagtaatgaa
    26881 attgttgcaa gagctcaggt tgtaggattt catgatgaaa aaacaatatt aagcttgatt
    26941 ggaaattctc gtggactttc acggcaaacg ttgattaagc ccactgccca gtttcttcat
    27001 acgcaagtcg gccgtggatt attgggagca gtagtcaatc ctttagggga ggttactgat
    27061 aagtttgctg ttacagataa cagtgaaatt ctttatcgac ctgtagataa tgctcctccg
    27121 ctatatagtg aaagggctgc aattgagaag ccttttttaa caggtattaa ggttattgat
    27181 tctttactca cgtgtggtga aggacagcga atggggattt ttgcgtcagc tggttgtggc
    27241 aaaacttttc tcatgaatat gctcattgaa catagtggtg ctgatatata tgttattggg
    27301 ttaattggtg agcgaggtcg agaggttact gaaacggttg attatttgaa aaactctgag
    27361 aaaaaaagca ggtgtgtttt agtatatgca acttcggatt actcttcggt tgatcgttgt
    27421 aatgctgcat atatagccac tgctatagcc gaatttttta ggactgaagg acataaagta
    27481 gcgcttttta ttgattcatt aacaaggtat gccagagcat tacgtgatgt ggccttagcc
    27541 gctggagaat cacctgccag aagaggctat ccggtttcgg tttttgatag cttacccaga
    27601 cttcttgaaa ggccaggaaa gttaaaggca ggtggctcta ttactgcatt ttacactgtt
    27661 cttttggagg atgatgattt tgctgatcca ttagctgaag aggtaagatc cattttagat
    27721 ggacatatat atttgagcag aaatctagcc caaaaaggac aatttcctgc aattgattcc
    27781 ttaaaaagta taagcagggt atttacacag gttgttgatg aaaaacatcg tattatggcc
    27841 gctgcatttc gggagctact ttctgaaata gaagagctaa ggacaattat tgactttggt
    27901 gaatacaaac cgggggagaa tgcctctcag gataaaatct acaacaaaat atctgttgtt
    27961 gagagttttc tgaagcaaga ttatcgactc ggttttactt atgagcagac aatggagctt
    28021 attggtgaaa caattagata aggttttaaa aatcaaagat aaatatcaac ggtctgttaa
    28081 attaattgaa gcacacatat taacactaaa aaaaaaactc tttgtatcga gatgtggagg
    28141 cattagataa aaggattata tacttcctgc aactggagaa tgacttagaa cctgtaggcg
    28201 ctcagagtgt tagtcagctt ttcaatacaa gacgtaagat cgctatagta aaaaagcata
    28261 ttatccaata tcagtctgaa cgaattcttc taaagggaag aattgaagaa attcaaaaag
    28321 atattgatga ggcaaatgct tctaaaagaa aactgcttca taaagagagt aaaatatgta
    28381 aacggattgg cttgattaaa agaaataact ttgccaaaca gttgatactt gatgagttat
    28441 cacaagagga tatgaagtat ggcattagat aatataaacc taaattttag tagtgacaaa
    28501 cagatagaaa aatgtgagaa actatcttca atagataata ttgatagtct cgttttgaag
    28561 aagaagagga aggttgaaat tcctgagtac tctttaattg catctaacta ttttactatt
    28621 gataagcact ttgaacataa gcatgataaa ggagaaattt atagtggcat taaaaatgcg
    28681 ttcgaactta gaaacgaacg agcgacatat tctgatattc cagaatcaat ggccattaaa
    28741 gaaaatattt tgataccaga tcaagatatc aaagcaaggg aaaaaataaa tatcggcgat
    28801 atgaggggga tcttttcata taataagagt ggaaatgcag acaagaactt cgaaagaagt
    28861 catacttctt ctgtaaaccc tgataatctg ctagaatctg ataatagaaa tggtcaaatt
    28921 ggtttaaaaa atcatagctt gtctattgat aagaatattg ctgacatcat ttctttacta
    28981 aatggaagtg ttgctaaatc atttgagctg cctgtaatga ataaaaatac tgcagacata
    29041 accccatcca tgtcattgca agaaaaatca atagttgaaa atgataaaaa tgtttttcaa
    29101 aaaaatagtg aaatgactta ccactttaaa cagtgggggg ctggacattc tgttagtatt
    29161 tcagtggagt ctggttcttt tgttctaaaa ccgtcagatc aatttgtagg aaataaactt
    29221 gacttaattt tgaaacaaga tgctgagggt aattacagat ttgatagcag tcaacataat
    29281 aaggggaata aaaataatag tacaggatat aatgaacaga gtgaagaaga atgctaagaa
    29341 ttaaacattt tgacgctaac gaaaaactac agattttata tgcaaagcaa ctctgcgagc
    29401 gtttttcgat tcagacattc aaaaataaat ttacaggcag tgaaagttta gtcactctta
    29461 cttccgtgtg tggggattgg gtaattcgta ttgatacatt atcttttttg aaaaaaaaat
    29521 acgaggtatt ttcaggattt tctacacaag aatctttact gcatttatca aaatgtgtct
    29581 ttatagagtc gtcatctgta ttttcgattc cagaactgtc tgataagatt actttccgga
    29641 tcacgaatga aatccagtat gcaactactg ggagtcattt atgctgtttt tcctcttctt
    29701 taggtattat ttattttgac aagatgccgg tattacgtaa tcaagtttct cttgactcat
    29761 tgcatcatct tttagagttt tgcttaggtt catctaatgt aaggctggct actttaaaaa
    29821 gaattcgcac tggtgatata atcatagttc agaaacttta taatttatta ttgtgtaatc
    29881 aagttattat tggggattat attgtgaatg ataataatga ggcaaaaatt aatctgtcag
    29941 aaagtaatgg tgagtcagaa cacacagaag tttctttggc attattcaat tatgatgata
    30001 tcaatgtaaa agtggacttt attcttttag aaaaaaatat gacaatcaat gaactaaaaa
    30061 tgtatgtaga aaacgaatta ttcaagtttc ccgatgacat agttaaacat gtaaatatta
    30121 aagtaaatgg ttctttggtt gggcatgggg aacttgtttc tattgaggat ggttatggta
    30181 tcgagattag ttcttggatg gtaaaggagt aatctcatgc tgagtgacat gtccctcatc
    30241 gctacacttt ctttttttac gctattacca tttttagttg ctgctggtac ctgttatata
    30301 aagttttcta ttgtttttgt aatggtacga aatgcacttg gtttgcagca ggttccatca
    30361 aatatgactc tcaatggtat tgcattgatt atggctttgt tcgtgatgaa acccattatt
    30421 gaagctggtt atgaaaatta tctaaatggc cctcaaaaat ttgatacaat atctgatatt
    30481 gtccgttttt ctgattctgg cttgatggaa tataagcaat acttgaaaaa gcatacagat
    30541 cttgagttag cacgtttttt ccaaaggtct gaagaagaaa atgctgattt aaagagtgca
    30601 gagaataatg attattcatt gttttccctc ttgcctgcat atgcgctaag tgaaatcaaa
    30661 gatgcgttca aaataggttt ttatttatat ttgccatttg ttgttgttga tcttgttatt
    30721 tcaagcattt tacttgcact tggtatgatg atgatgagtc caatcacaat atcagtacca
    30781 ataaaacttg tattatttgt tgcgcttgat gggtggggta tcctgtctaa ggccttgatt
    30841 gaacaatata tcaatattcc tgcttagatg atggaggtaa tgtgtctgat atagtttata
    30901 tgggtaataa ggctctttat ttaatcctta tcttttcctt atggccagta ggtatagcta
    30961 cggttattgg attatctatt ggtttgttac agacagtgac tcaactccaa gagcagacac
    31021 ttccttttgg tataaagctt ataggtgtct caatatcttt gctacttctt tctggatggt
    31081 atggtgaggt tttattgtct ttttgtcatg aaataatgtt tttaattaag agtggggttt
    31141 gatggacatt tcaagctggt tcgaaagtat tcatgtgttt ttaatactcc tgaacggcgt
    31201 tttttttaga ttggctccat tatttttctt tcttccattt ttaaataacg gtataatttc
    31261 tccatctatt agaatacctg tgatttttct tgttgcatca ggattaatta cttctggtaa
    31321 ggtagacata ggttcttctg tttttgaaca tgtttatttc cttatgttca aggaaataat
    31381 tgttggcctc cttctctctt tttgcttgtc tcttcccttt tggatatttc atgctgttgg
    31441 tagcattatt gacaaccagc gtggggcaac gcttagtagt tcaattgatc ctgccaatgg
    31501 tgttgatacg tctgagttgg caaaattttt caatcttttt tctgcagttg tatttctata
    31561 cagtggtggt atggtcttta ttttagaatc catacaattg tcttataata tatgcccgtt
    31621 attttctcaa tgttctttcc gtgtctcaaa tatcttaaca tttctgactt tattggcaag
    31681 tcaggctgtt attttagcca gtcctgttat gatagtattg ttactatcag aagtattact
    31741 tggtgtatta tcgagatttg ctccgcagat gaatgctttt tccgtatcat taactattaa
    31801 aagtttactt gcaatattta ttattttcat ctgttcttct actatttact tttctaaagt
    31861 tcaatttttc ctcggtgaac ataaattttt cacaaatcta tttgttagat aaaatattat
    31921 ggcaaataaa acagaaaagc cgacacctaa aaaactaaag gatgccgcaa aaaaaggaca
    31981 gtcatttaaa tttaaggatt taacgactgt tgttattatt ctggtaggga catttactat
    32041 aatatcattc ttttccttaa gtgatgtaat gctcttatac agatatgtaa taattaatga
    32101 cttcgaaatt aatgagggta aatacttttt tgcagtggtt attgtctttt ttaagataat
    32161 tggcttccca ctttttttct gtgttctttc ggctgtgttg ccaacattgg ttcaaacaaa
    32221 gtttgttctt gcgactaaag ctatcaagat tgatttttca gtattaaacc ctgttaaagg
    32281 gttaaaaaaa atatttagta taaagacaat aaaagaattt ttcaaaagca ttctgcttct
    32341 tattattcta gcattaacaa cctatttctt ttggattaat gaccgaaaaa taattttttc
    32401 tcaggtgttt tctagtgttg atggcttata tcttatttgg gggaggctgt ttaaggatat
    32461 aatattattt ttcttggcat tttctattct tgttattatt cttgactttg tgattgagtt
    32521 cattttatac atgaaagata tgatgatgga taaacaggag ataaaaagag aatatataga
    32581 gcaagaggga cactttgaga caaagtcgag aaggcgtgag ttgcatatcg agattctttc
    32641 agagcagact aaatctgata tacgtaattc aaaattagtg gtaatgaacc cgactcatat
    32701 tgcaattggt atttatttta atccagaaat agcgcctgca ccttttattt ctctcattga
    32761 aactaaccag tgtgccttgg ctgtcagaaa atatgcaaat gaagttggta taccgactgt
    32821 gcgtgatgtg aaattagcta gaaaactata taaaacacat acaaaatata gttttgttga
    32881 ttttgaacac ttggatgaag tcctacgtct tattgtttgg cttgagcagg ttgaaaacac
    32941 tcattaataa gtaaaggaga tgtgttatat gggagttaat ttctgtaata aaataggtat
    33001 tgatcagtct gaatttgaaa tagaatcttc catcattaac tccattgcta atgaggtatt
    33061 gaacccaata tctttcctta gcaataagga tataataaat gttttactca gaaagatttc
    33121 atctgaatgt gacctggtta gaaaagacat ttatcgttgt gctctggagt tggtcgttga
    33181 aaaaacacct gatgatttat aatcattcat aataactctt ttgctgatat aaatattttt
    33241 gttgaagata tttatgtagt cacaggatca attgtagaaa attgttatgt ttttgttagg
    33301 aactgcacta aataatatat gataagacag caaaaacgac tgaccataat ccttctttta
    33361 ttaggagttg ataagagaga ctattcatcc tgtaatgtta aaactctgtt atatagtatt
    33421 cgcgattatg caaagtctgt aaatgatcat gagatactaa ccgagagtaa tcgtttatta
    33481 tctcattgca tttcagatag taacggagct ttctttaaaa gtagtaaata cgttcccctt
    33541 aagtacttac gtaaaaggag aatagcacga aagataccaa atgattagca ctttgctagt
    33601 aagcttgctg gtaaactata ttgaccgttg ggtttgtttt gagactgtaa tttaaattgt
    33661 gtaattgcct gtttttgata tgtttactcc aacaacggag acaggtaaat tatggacgaa
    33721 aagaagctta aagcccttgc ggctgaactg gctaaaggcc tcaaaactga ggccga
//
LOCUS       DAJTRC010000039        33447 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.039, whole genome shotgun sequence.
ACCESSION   DAJTRC010000039 DAJTRC010000000
VERSION     DAJTRC010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33447)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33447)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33447
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.039"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            94..1590
                     /gene="ravA"
                     /locus_tag="OO357_001958"
     CDS             94..1590
                     /gene="ravA"
                     /locus_tag="OO357_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418202.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA"
                     /protein_id="HCR5886917.1"
                     /translation="MAHPHLLAERISRLSSSLEKGLYERSHAIRLCLLAALSGESVFL
                     LGPPGIAKSLIARRLKFAFQNARAFEYLMTRFSTPEEVFGPLSIQALKDEGRYERLTS
                     GYLPEAEIVFLDEIWKAGPAILNTLLTAINERQFRNGAHVEKIPMRLLVAASNELPEA
                     DSSLEALYDRMLIRLWLDKVQDKANFRSMLTSQQDENDNPVPDALQVTDEEYERWQKE
                     IGEITLPDHVFELIFILRQQLDKLPDAPYVSDRRWKKAIRLLQASAFFSGRSAVAPVD
                     LILLKDCLWYDAQSLNLIQQQIDVLMTGHAWQQQGMLTRLGAIVQRHLQLQQQQSDKT
                     ALTLIRLGGIFSRRQQYQLPVNVTASTLTLLLQKPLKLHDMEVVHISFERSALEQWLS
                     KGGEIRGKLNGIGFAQKLNLEVDSTQHLVVRDVSLQGSTLALPGSSAEGLPGEIKQQL
                     EELESDWRKQHALFSEQQKCLFIPGDWLGRIEDSLQDVGAQIRQAQQC"
     gene            1584..3035
                     /gene="viaA"
                     /locus_tag="OO357_001959"
     CDS             1584..3035
                     /gene="viaA"
                     /locus_tag="OO357_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase RavA stimulator ViaA"
                     /protein_id="HCR5886918.1"
                     /translation="MLTLDTLNVMLAVSEEGLIEEMIIALLASPQLAVFFEKFPRLKA
                     AITDDVPRWREALRSRLKDARVPPELTEEVMCYQQSQLLSTPQFIVQLPQILDLLHRL
                     NSPWAEQARQLVDANSTITSALHTLFLQRWRLSLIVQATTLNQQLLEEEREQLLSEVQ
                     ERMTLSGQLEPILADNNTAAGRLWDMSAGQLKRGDYQFIVKYGEFLNEQPELKRLAEQ
                     LGRSREAKSIPRNDAQMETFRTMVREPATVPEQVDGLQQSDDILRLLPPELATLGITE
                     LEYEFYRRLVEKQLLTYRLHGESWREKVFERPVVHKDYDEQPRGPFIVCVDTSGSMGG
                     FNEQCAKAFCLALMRIALAENRRCYIMLFSTEIVRYELSGPQGIEQAIRFLSQQFRGG
                     TDLASCFRAIMERLQSREWFDADAVVISDFIAQRLPDDVTSKVKELQRVHQHRFHAVA
                     MSAHGKPGIMRIFDHIWRFDTGMRSRLLRRWRR"
     gene            complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO357_001960"
     CDS             complement(3040..4032)
                     /gene="asnA"
                     /locus_tag="OO357_001960"
                     /EC_number="6.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--ammonia ligase"
                     /protein_id="HCR5886919.1"
                     /translation="MKTAYIAKQRQISFVKSHFSRQLEERLGLIEVQAPILSRVGDGT
                     QDNLSGCEKAVQVKVKALPDAQFEVVHSLAKWKRQTLGQHDFSAGEGLYTHMKALRPD
                     EDRLSPLHSVYVDQWDWERVMGDGERQLSTLKSTVEAIWAGIKATEAAVNEEFGLAPF
                     LPDQIHFVHSQELLSRYPDLDAKGRERAIAKDLGAVFLVGIGGKLSDGHRHDVRAPDY
                     DDWSTPSELGHAGLNGDILVWNPVLEDAFELSSMGIRVDADTLKHQLALTGDEDRLQL
                     EWHQALLRGEMPQTIGGGIGQSRLTMLLLQLPHIGQVQCGVWPAAVRESVPSLL"
     gene            4184..4642
                     /gene="asnC"
                     /locus_tag="OO357_001961"
     CDS             4184..4642
                     /gene="asnC"
                     /locus_tag="OO357_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator AsnC"
                     /protein_id="HCR5886920.1"
                     /translation="MENYLIDNLDRGILEALMGNARTAYAELAKQFGVSPGTIHVRVE
                     KMKQAGIITGARIDVSPKQLGYDVGCFIGIILKSAKDYPSALAKLESLDEVTEAYYTT
                     GHYSIFIKVMCRSIDALQHVLINKIQTIDEIQSTETLIVLQNPIMRTIKP"
     gene            4732..5175
                     /gene="mioC"
                     /locus_tag="OO357_001962"
     CDS             4732..5175
                     /gene="mioC"
                     /locus_tag="OO357_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein MioC"
                     /protein_id="HCR5886921.1"
                     /translation="MADITLISGSTLGGAEYVAEHLAEKLEEVGFTTETLHGPLLEDL
                     SASGIWLVISSTHGAGDIPDNLSPFYEALQEQKPDLSAVRFGTIGIGSREYDTFCGAI
                     DKLEAELKNSGAKQTGETLKINILDHDIPEDPAEEWLGSWINLLK"
     gene            5554..7443
                     /gene="mnmG"
                     /locus_tag="OO357_001963"
     CDS             5554..7443
                     /gene="mnmG"
                     /locus_tag="OO357_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000499793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HCR5886922.1"
                     /translation="MFYPDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQ
                     MSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRV
                     RYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
                     GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVGRLKTGTPPRIDARTIDFSVLAQQ
                     HGDNPMPVFSFMGNASLHPQQVPCYITHTNEKTHDVIRSNLDRSPMYAGVIEGVGPRY
                     CPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENA
                     KIVRPGYAIEYDFFDPRDLKPTLESKFIQGLFFAGQINGTTGYEEAAAQGLLAGLNAA
                     RLSADKEGWAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTE
                     IGRELGLVDDERWARFNEKLENIERERQRLKSTWVTPSAEAAAEVNAHLTAPLSREAS
                     GEDLLRRPEMTYEKLTTLTPFAPALTDEQAAEQVEIQVKYEGYIARQQDEIEKQLRNE
                     NTLLPATLDYRQISGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGM
                     LRRSA"
     gene            7507..8130
                     /gene="rsmG"
                     /locus_tag="OO357_001964"
     CDS             7507..8130
                     /gene="rsmG"
                     /locus_tag="OO357_001964"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HCR5886923.1"
                     /translation="MLNKLSLLLKDAGISLTDHQKNQLIAYVNMLHKWNKAYNLTSVR
                     DPNEMLVRHILDSIVVAPYLQGERFIDVGTGPGLPGIPLSIVRPEAHFTLLDSLGKRV
                     RFLRQVQHELKLENIEPVQSRVEEFPSEPPFDGVISRAFASLNDMVSWCHHLPGEQGR
                     FYALKGQMPEDEIALLPEEYQVESVVKLQVPALDGERHLVVIKANKI"
     gene            8747..9127
                     /gene="atpI"
                     /locus_tag="OO357_001965"
     CDS             8747..9127
                     /gene="atpI"
                     /locus_tag="OO357_001965"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418195.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit I"
                     /protein_id="HCR5886924.1"
                     /translation="MSVSLVSRNVARKLLLVQLLVVIASGLLFSLKDPFWGVSAISGG
                     LAVFLPNVLFMIFAWRHQAHTPAKGRVAWTFAFGEAFKVLAMLVLLVVALAVLKAVFL
                     PLIVTWVLVLVVQILAPAVINNKG"
     gene            9136..9951
                     /gene="atpB"
                     /locus_tag="OO357_001966"
     CDS             9136..9951
                     /gene="atpB"
                     /locus_tag="OO357_001966"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HCR5886925.1"
                     /translation="MASENMTPQDYIGHHLNNLQLDLRTFSLVDPQNPPATFWTINID
                     SMFFSVVLGLLFLVLFRSVAKKATSGVPGKFQTAIELVIGFVNGSVKDMYHGKSKLIA
                     PLALTIFVWVFLMNLMDLLPIDLLPYIAEHVLGLPALRVVPSADVNVTLSMALGVFIL
                     ILFYNIKMKGIGGFTKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAG
                     ELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH"
     gene            9998..10237
                     /gene="atpE"
                     /locus_tag="OO357_001967"
     CDS             9998..10237
                     /gene="atpE"
                     /locus_tag="OO357_001967"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004246596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HCR5886926.1"
                     /translation="MENLNMDLLYMAAAVMMGLAAIGAAIGIGILGGKFLEGAARQPD
                     LIPLLRTQFFIVMGLVDAIPMIAVGLGLYVMFAVA"
     gene            10299..10769
                     /gene="atpF"
                     /locus_tag="OO357_001968"
     CDS             10299..10769
                     /gene="atpF"
                     /locus_tag="OO357_001968"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001052215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HCR5886927.1"
                     /translation="MNLNATILGQAIAFVLFVLFCMKYVWPPLMAAIEKRQKEIADGL
                     ASAERAHKDLDLAKASATDQLKKAKAEAQVIIEQANKRRSQILDEAKAEAEQERTKIV
                     AQAQAEIEAERKRAREELRKQVAILAVAGAEKIIERSVDEAANSDIVDKLVAEL"
     gene            10784..11317
                     /gene="atpH"
                     /locus_tag="OO357_001969"
     CDS             10784..11317
                     /gene="atpH"
                     /locus_tag="OO357_001969"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HCR5886928.1"
                     /translation="MSEFITVARPYAKAAFDFAVEHQSVERWQDMLTFAAEVTKNEQM
                     AELLSGALAPETLAESFIAVCGEQLDENGQNLIRVMAENGRLNALPDVLEQFIHLRAV
                     SEATAEVDVISAAALSEQQLAKISAAMEKRLSRKVKLNCKIDKSVMAGVIIRSGDMVI
                     DGSVRGRLERLADVLQS"
     gene            11330..12871
                     /gene="atpA"
                     /locus_tag="OO357_001970"
     CDS             11330..12871
                     /gene="atpA"
                     /locus_tag="OO357_001970"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001176751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HCR5886929.1"
                     /translation="MQLNSTEISELIKQRIAQFNVVSEAHNEGTIVSVSDGVIRIHGL
                     ADCMQGEMISLPGNRYAIALNLERDSVGAVVMGPYADLAEGMKVKCTGRILEVPVGRG
                     LLGRVVNTLGAPIDGKGPLDHDGFSAVEAIAPGVIERQSVDQPVQTGYKAVDSMIPIG
                     RGQRELIIGDRQTGKTALAIDAIINQRDSGIKCIYVAIGQKASTISNVVRKLEEHGAL
                     ANTIVVVATASESAALQYLAPYAGCAMGEYFRDRGEDALIIYDDLSKQAVAYRQISLL
                     LRRPPGREAFPGDVFYLHSRLLERAARVNAEYVEAFTKGEVKGKTGSLTALPIIETQA
                     GDVSAFVPTNVISITDGQIFLETNLFNAGIRPAVNPGISVSRVGGAAQTKIMKKLSGG
                     IRTALAQYRELAAFSQFASDLDDATRKQLDHGQKVTELLKQKQYAPMSVAQQSLVLFA
                     AERGYLADVELSKIGSFEAALLAYVDRDHAPLMQEINQTGGYNDEIEGKLKGILDSFK
                     ATQSW"
     gene            12922..13785
                     /gene="atpG"
                     /locus_tag="OO357_001971"
     CDS             12922..13785
                     /gene="atpG"
                     /locus_tag="OO357_001971"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HCR5886930.1"
                     /translation="MAGAKEIRSKIASVQNTQKITKAMEMVAASKMRKSQDRMAASRP
                     YAETMRKVIGHLAHGNLEYKHPYLEDRDVKRVGYLVVSTDRGLCGGLNINLFKKLLAE
                     MKTWTDKGVQCDLAMIGSKGVSFFNSVGGNVVAQVTGMGDNPSLSELIGPVKVMLQAY
                     DEGRLDKLYIVSNKFINTMSQVPTISQLLPLPASDDDDLKHKSWDYLYEPDPKALLDT
                     LLRRYVESQVYQGVVENLASEQAARMVAMKAATDNGGSLIKELQLVYNKARQASITQE
                     LTEIVSGAAAV"
     gene            13812..15194
                     /gene="atpD"
                     /locus_tag="OO357_001972"
     CDS             13812..15194
                     /gene="atpD"
                     /locus_tag="OO357_001972"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017800291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HCR5886931.1"
                     /translation="MATGKIVQVIGAVVDVEFPQDAVPRVYDALEVQNGNERLVLEVQ
                     QQLGGGIVRTIAMGSSDGLRRGLDVKDLEHPIEVPVGKATLGRIMNVLGEPVDMKGEI
                     GEEERWAIHRAAPSYEELSNSQELLETGIKVIDLMCPFAKGGKVGLFGGAGVGKTVNM
                     MELIRNIAIEHSGYSVFAGVGERTREGNDFYHEMTDSNVIDKVSLVYGQMNEPPGNRL
                     RVALTGLTMAEKFRDEGRDVLLFVDNIYRYTLAGTEVSALLGRMPSAVGYQPTLAEEM
                     GVLQERITSTKTGSITSVQAVYVPADDLTDPSPATTFAHLDATVVLSRQIASLGIYPA
                     VDPLDSTSRQLDPLVVGQEHYDTARGVQSILQRYQELKDIIAILGMDELSEEDKLVVA
                     RARKIQRFLSQPFFVAEVFTGSPGKYVSLKDTIRGFKGIMEGEYDHLPEQAFYMVGSI
                     EEAVEKAKKL"
     gene            15215..15634
                     /gene="atpC"
                     /locus_tag="OO357_001973"
     CDS             15215..15634
                     /gene="atpC"
                     /locus_tag="OO357_001973"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001251972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HCR5886932.1"
                     /translation="MAMTYHLDVVSAEQQMFSGLVEKIQVTGSEGELGIYPGHAPLLT
                     AIKPGMIRIVKQHGHEEFIYLSGGILEVQPGNVTVLADTAIRGQDLDEARAMEAKRKA
                     EEHISSSHGDVDYAQASAELAKAIAQLRVIELTKKAM"
     gene            15985..17355
                     /gene="glmU"
                     /locus_tag="OO357_001974"
     CDS             15985..17355
                     /gene="glmU"
                     /locus_tag="OO357_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HCR5886933.1"
                     /translation="MLNNAMSVVILAAGKGTRMYSDLPKVLHTLAGKAMVQHVIDAAN
                     ELGAAHVHLVYGHGGDLLKQALKDDNLNWVLQAEQLGTGHAMQQAAPFFADDEDILML
                     YGDVPLISVETLQRLRDAKPQGGIGLLTVKLDDPTGYGRITRENGKVTGIVEHKDATD
                     EQRQIQEINTGILIANGADMKRWLAKLTNNNAQGEYYITDIIALAYQEGREIVAVHPQ
                     RLSEVEGVNNRLQLSRLERVYQSEQAEKLLLAGVMLRDPARFDLRGTLTHGRDVEIDT
                     NVIIEGNVTLGHRVKIGTGCVIKNSVIGDDCEISPYTVVEDANLAAACTIGPFARLRP
                     GAELLEGAHVGNFVEMKKARLGKGTKAGHLTYLGDAEIGDNVNIGAGTITCNYDGANK
                     FKTIIGDDVFVGSDTQLVAPVTVGKGATIAAGTTVTRNVGENALAISRVPQTQKEGWR
                     RPVKKK"
     gene            17517..19346
                     /gene="glmS"
                     /locus_tag="OO357_001975"
     CDS             17517..19346
                     /gene="glmS"
                     /locus_tag="OO357_001975"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HCR5886934.1"
                     /translation="MCGIVGAIAQRDVAEILLEGLRRLEYRGYDSAGLAVVDAEGHMT
                     RLRRLGKVQMLAQAAEEHPLHGGTGIAHTRWATHGEPSEANAHPHVSEHIVVVHNGII
                     ENHEPLREELKARGYTFVSETDTEVIAHLVNWELKQGGTLREAVLRAIPQLRGAYGTV
                     IMDSRHPDTLLAARSGSPLVIGLGMGENFIASDQLALLPVTRRFIFLEEGDIAEITRR
                     SVNIFDKTGAEVKRQDIESNLQYDAGDKGIYRHYMQKEIYEQPNAIKNTLTGRISHGQ
                     VDLSELGPNADELLSKVEHIQILACGTSYNSGMVSRYWFESLAGIPCDVEIASEFRYR
                     KSAVRRNSLMITLSQSGETADTLAGLRLSKELGYLGSLAICNVPGSSLVRESDLALMT
                     NAGTEIGVASTKAFTTQLTVLLMLVAKLSRLKGLDASIEHDIVHGLQALPSRIEQMLS
                     QDKRIEALAEDFSDKHHALFLGRGDQYPIALEGALKLKEISYIHAEAYAAGELKHGPL
                     ALIDADMPVIVVAPNNELLEKLKSNIEEVRARGGQLYVFADQDAGFVSSDNMHIIEMP
                     HVEEVIAPIFYTVPLQLLAYHVALIKGTDVDQPRNLAKSVTVE"
     gene            19508..20329
                     /locus_tag="OO357_001976"
     CDS             19508..20329
                     /locus_tag="OO357_001976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heteromeric transposase endonuclease subunit
                     TnsA"
                     /protein_id="HCR5886935.1"
                     /translation="MAKANSSFSEVQIARRIKEGRGQGHGKDYIPWLTVQEVPSSGRS
                     HRIYSHKTGRVHHLLSDLELAVFLSLEWESSVLDIREQFPLLPSDTRQIAIDSGIKHP
                     VIRGVDQVMSTDFLVDCKDGPFEQFAIQVKPAAALQDERTLEKLELERRYWQQKQIPW
                     FIFTDKEINPVVKENIEWLYSVKTEEVSAELLAQLSPLAHILQEKGDENIINVCKQVD
                     IAYDLELGKTLSEIRALTANGFIKFNIYKSFRANKCADLCISQVVNMEELRYVAN"
     gene            20316..22424
                     /locus_tag="OO357_001977"
     CDS             20316..22424
                     /locus_tag="OO357_001977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000267723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
                     /protein_id="HCR5886936.1"
                     /translation="MWQINEVVLFDNDPYRILAIEDGQVVWMQISADKGVPQARAELL
                     LMQYLDEGRLVRTDDPYVHLDLEEPSVDSVSFQKREEDYRKILPIINSKDRFDPKVRS
                     ELVEHVVQEHKVTKATVYKLLRRYWQRGQTPNALIPDYKNSGAPGERRSATGTAKIGR
                     AREYGKGEGTKVTPEIERLFRLTIEKHLLNQKGTKTTVAYRRFVDLFAQYFPRIPQED
                     YPTLRQFRYFYDREYPKAQRLKSRVKAGVYKKDVRPLSSTATSQALGPGSRYEIDATI
                     ADIYLVDHHDRQKIIGRPTLYIVIDVFSRMITGFYIGFENPSYVVAMQAFVNACSDKT
                     AICAQHDIEISSSDWPCVGLPDVLLADRGELMSHQVEALVSSFNVRVESAPPRRGDAK
                     GIVESTFRTLQAEFKSFAPGIVEGSRIKSHGETDYRLDASLSVFEFTQIILRTILFRN
                     NHLVMDKYDRDADFPTDLPSIPVQLWQWGMQHRTGSLRAVEQEQLRVALLPRRKVSIS
                     SFGVNLWGLYYSGSEILREGWLQRSTDIARPQHLEAAYDPVLVDTIYLFPQVGSRVFW
                     RCNLTERSRQFKGLSFWEVWDIQAQEKHNKANAKQDELTKRRELEAFIQQTIQKANKL
                     TPSTTEPKSTRIKQIKTNKKEAVTSERKKRAEHLKPSSSGDEAKVIPFNAVEADDQED
                     YSLPTYVPELFQDPPEKDES"
     gene            22421..24088
                     /locus_tag="OO357_001978"
     CDS             22421..24088
                     /locus_tag="OO357_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001276994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5886937.1"
                     /translation="MSATRIQAVYRDTGVEAYRDNPFIEALPPLQESVNSAASLKSSL
                     QLTSSDLQKSRVIRAHTICRIPDDYFQPLGTHLLLSERISVMIRGGYVGRNPKTGDLQ
                     KHLQNGYERVQTGELETFRFEEARSTAQSLLLIGCSGSGKTTSLHRILATYPQVIYHR
                     ELNVEQVVYLKIDCSHNGSLKEICLNFFRALDRALGSNYERRYGLKRHGIETMLALMS
                     QIANAHALGLLVIDEIQHLSRSRSGGSQEMLNFFVTMVNIIGVPVMLIGTPKAREIFE
                     ADLRSARRGAGFGAIFWDPIQQTQRGKPNQEWIAFTDNLWQLQLLQRKDALLSDEVRD
                     VWYELSQGVMDIVVKLFVLAQLRALALGNERITAGLLRQVYQDELKPVHPMLEALRSG
                     IPERIARYSDLVVPEIDKRLIQLQLDIAAIQEQTPEEKALQELDTEDQRHLYLMLKED
                     YDSSLLIPTIKKAFSQNPTMTRQKLLPLVLQWLMEGETVVSELEKPSKSKKVSAIKVV
                     KPSDWDSLPDTDLRYIYSQRQPEKTMHERLKGKGVIVDMASLFKQAG"
     gene            24091..25617
                     /locus_tag="OO357_001979"
     CDS             24091..25617
                     /locus_tag="OO357_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001243518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TniQ family protein"
                     /protein_id="HCR5886938.1"
                     /translation="MRNFPVPYSNELIYSTIARAGVYQGIVSPKQLLDEVYGNRKVVA
                     TLGLPSHLGVIARHLHQTGRYAVQQLIYEHTLFPLYAPFVGKERRDEAIRLMEYQAQG
                     AVHLMLGVAASRVKSDNRFRYCPDCVALQLNRYGEAFWQRDWYLPALPYCPKHGALVF
                     FDRAVDDHRHQFWALGHTELLSDYPKDSLSQLTALAAYIAPLLDAPRAQELSPSLEQW
                     TLFYQRLAQDLGLTKSKHIRHDLVAERVRQTFSDEALEKLDLKLAENKDTCWLKSIFR
                     KHRKAFSYLQHSIVWQALLPKLTVIEALQQASALTEHSITTRPVSQSVQPNSEDLSVK
                     HKDWQQLVHKYQGIKAARQSLEGGVLYAWLYRHDRDWLVHWNQQHQQERLAPAPRVDW
                     NQRDRIAVRQLLRIIKRLDSSLDHPRATSSWLLKQTPNGTSLAKNLQKLPLVALCLKR
                     YSESVEDYQIRRISQAFIKLKQEDVELRRWRLLRSATLSKERITEEAQRFLEMVYGEE
                     "
     gene            25618..27234
                     /locus_tag="OO357_001980"
     CDS             25618..27234
                     /locus_tag="OO357_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000251878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator"
                     /protein_id="HCR5886939.1"
                     /translation="MVRLATFNDNVQVVHIGHLFRNSGHKEWRIFVWFNPMQERKWTR
                     FTHLPLLSRAKVVNSTTKQINKADRVIEFEASDLQRAKIIDFPNLSSFASVRNKDGAQ
                     SSFIYEAETPYSKTRYHIPQLELARSLFLINSYFCRSCLSSTALQQEFDVQYEVERDH
                     LEIRILPSSSFPKGALELSAVVQLLVWLFSDQDVMDSYESIFRHYQQNREIKNGVESW
                     CFSFDPPPMQGWKLHVKGRSSNEDKDYLVEEIVGLEINAMLPSTTAISHASFQEKEAG
                     DGSTQHIAVSTESVVDDEHLQLDDEETANIDTDTRVIEAEPTWISFSRPSRIEKSRRA
                     RKSSQTILEKEEATTSENSNLVSTDEPHLGGVLAAADVGGKQDATNYNSIFANRFAAF
                     DELLSILKTKFACRVLFEETLVLPKVGRSRLHLCKDGSPRVIKAVGVQRNGSEFVLLE
                     VDASDGVKMLSTKVLSGVDSETWRNDFEKIRRGVVKSSLNWPNSLFDQLYGQDGHRGV
                     NHPKGLGELQVSREDMEGWAERVVREQFTH"
     gene            complement(27290..27442)
                     /locus_tag="OO357_001981"
                     /pseudo
     CDS             complement(27290..27442)
                     /locus_tag="OO357_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069623.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(27465..27842)
                     /locus_tag="OO357_001982"
     CDS             complement(27465..27842)
                     /locus_tag="OO357_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001271300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886940.1"
                     /translation="MSAAKALAYALCDKLESKKVDEVVRNLVSSGWNIREVAWEASSF
                     DHEMPYFDTGRDFDIGFPDTLSSKIEFLTANGSIVQARARLHFKQALIFAKASKHVAA
                     LKNSLDYYYGSSVIPPQNHGGYL"
     gene            complement(28252..28623)
                     /locus_tag="OO357_001983"
     CDS             complement(28252..28623)
                     /locus_tag="OO357_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000119696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886941.1"
                     /translation="MSYEPPRDGYTNGYIVDVMYDNRPSKYPAVFRTYVRIPDYVELD
                     NAEELADAIIREVELKEAWLTRVLGNSADPDASVGKWDRRLAQCGGIDEMSSGVQATY
                     FSPNGQLVGLVAYIDAEWMDE"
     gene            complement(28684..29181)
                     /locus_tag="OO357_001984"
     CDS             complement(28684..29181)
                     /locus_tag="OO357_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5886942.1"
                     /translation="MRDLLKVVLSLVLAMASGQALADLPIVLVDEARLPYDYSPSNYD
                     ISPSNYDNSISNYDNSPSNYDNSESNYDNSSSNYDNSRNGNRRLIYSANGSRTFAGYY
                     VIANNGTTNFFSTSGKRMFYTPKGGRGVYGGKDGSFCGALVVINGQFSLALTDNGLKI
                     MYLSN"
     gene            complement(29257..30045)
                     /gene="aadA1"
                     /locus_tag="OO357_001985"
     CDS             complement(29257..30045)
                     /gene="aadA1"
                     /locus_tag="OO357_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5886943.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
     gene            complement(30103..30627)
                     /gene="sat2"
                     /locus_tag="OO357_001986"
     CDS             complement(30103..30627)
                     /gene="sat2"
                     /locus_tag="OO357_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5886944.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            complement(30722..31195)
                     /locus_tag="OO357_001987"
     CDS             complement(30722..31195)
                     /locus_tag="OO357_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5886945.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            31527..32063
                     /locus_tag="OO357_001988"
     CDS             31527..32063
                     /locus_tag="OO357_001988"
                     /inference="COORDINATES: protein motif:HMM:NF024886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
                     /protein_id="HCR5886946.1"
                     /translation="MSNSPFLNSIRTDMRQKGYALKTEKTYLHWIKRFILFHKKRHPQ
                     TMGSEEVRLFLSSLANSRHVAINTQKIALNALAFLYNRFLQQPLGDIDYIPASKPRRL
                     PSVISANEVQRILQVMDTRNQVIFTLLYGAGLRINECLRLRVKDFDFDNGCITVHDGK
                     GGKSRNSLLPTRLIPAIK"
     gene            32178..32504
                     /locus_tag="OO357_001989"
     CDS             32178..32504
                     /locus_tag="OO357_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000497519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5886947.1"
                     /translation="MFVFPSSTLCNHPYNGKLCRHHLHDSVARKALKAAVQKAGIVSK
                     RVTCHTFRHSFATHLLQAGRDIRTVQELLGHNDVKTTQIYTHVLGQHFAGTTSPADGL
                     MLLINQ"
     gene            complement(32692..32931)
                     /locus_tag="OO357_001990"
     CDS             complement(32692..32931)
                     /locus_tag="OO357_001990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001353740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1819 family protein"
                     /protein_id="HCR5886948.1"
                     /translation="MTPFRYNSDLTSGSLQTRECRIITGLLLQELDEAAWDKAMYKEN
                     VLQKRTQSTVRRISSALRKRLEHLSSDFWAFAFLC"
BASE COUNT         8674 a         7805 c         8447 g         8521 t
ORIGIN      
        1 attacccccc tttgagacta ttttagtcac cagcaagaaa cgtctatact cgcaatttac
       61 gcagaacttt tgacgaaagg acgccacttc attatggctc accctcattt attagcggaa
      121 agaatttccc gcctgagcag ttcgttggaa aaggggcttt atgaacgtag ccacgccatc
      181 cgcttgtgtt tattagcggc attaagtggt gaaagtgtgt tcctccttgg cccgccaggt
      241 attgccaaaa gtttgatcgc ccggcgctta aaattcgcct ttcagaatgc ccgcgcgttt
      301 gaatatctga tgacccgctt ctccacgccg gaagaagttt ttggtcccct ttctattcag
      361 gcgctaaaag atgaagggcg ctatgaacgt ttaaccagcg gttacctgcc ggaagcagaa
      421 atcgtctttc tggatgagat ctggaaagcg ggcccggcaa ttcttaatac cttgctcacc
      481 gccattaacg agcgccagtt ccgcaacggt gcacacgtag aaaaaatccc gatgcgtcta
      541 ctggtggcgg cctccaacga gctgccggaa gcagacagca gtctggaagc gttatatgac
      601 cgcatgctga ttcgtctatg gttagataaa gtgcaagata aagcgaattt ccgctccatg
      661 ctgaccagtc aacaggatga aaacgacaat ccggttcctg atgccttgca ggtcacagat
      721 gaagaatatg aacgctggca gaaagagatt ggtgaaatta cgctgcccga tcatgtattt
      781 gagctgattt ttattctgcg ccagcaactg gataaattac cggatgcgcc ttatgtctcg
      841 gatcgtcgct ggaaaaaagc gatccgctta ttgcaggcca gcgccttttt tagcggtcgc
      901 agtgctgttg ccccggttga tctcattttg ctgaaagatt gcctgtggta tgacgcgcaa
      961 agcctgaatt tgatacaaca acaaattgat gtattgatga ccggtcatgc ctggcaacag
     1021 caagggatgt tgacccgcct gggcgcgatt gtgcaacgtc acctgcaact ccagcagcaa
     1081 caaagcgata aaacagcctt aacgttaatt cgtctgggcg gcattttcag ccgtcgtcag
     1141 cagtatcaac ttcctgttaa cgttactgct tccactctga ctctgctgct gcaaaaaccg
     1201 ttaaaactgc atgatatgga agtggttcat atctcctttg agcgtagcgc gctggaacag
     1261 tggctgagta aaggtggtga aattcgcggc aaactgaacg gtatcggctt tgcccagaaa
     1321 ctgaatctgg aagttgatag cacccaacat cttgttgtac gcgatgtaag tttacaaggc
     1381 agtacgctgg cacttcccgg ttcatcggct gaaggtctgc caggtgaaat aaaacaacaa
     1441 ctggaagagc ttgaaagcga ctggcgtaag caacacgctt tattcagcga acagcaaaaa
     1501 tgtctgttta tccctggcga ctggttaggt cgcattgaag acagcctaca ggatgtcggt
     1561 gcacagattc gtcaggcaca acaatgctaa cgctggatac gcttaatgtg atgctggccg
     1621 tcagcgaaga gggattgatc gaagagatga tcatcgcact gctggcctca ccgcagctgg
     1681 cggtcttctt tgaaaaattc ccacgactga aggcggcaat cactgatgat gttccccgct
     1741 ggcgtgaggc gctgcgcagt cggctgaaag atgcccgagt cccgccggaa ctcaccgaag
     1801 aggtgatgtg ctatcagcaa agccagctcc tctccacgcc gcagtttatt gtgcagctac
     1861 cacagatcct ggacttactg catcgtctga attccccatg ggcagaacaa gcccggcagt
     1921 tggttgatgc taacagcaca ataacttcag cgttacacac gctttttctc cagcgttggc
     1981 gtttaagtct gatcgtgcaa gcaacgacgt taaatcaaca gctattagaa gaagaacgcg
     2041 aacaactgtt aagtgaagtt caggaacgca tgacgctgag cggacaactt gaaccgattc
     2101 tcgcagataa caataccgca gctggtcgtc tgtgggatat gagcgccggt caacttaaac
     2161 gtggcgacta tcagtttatt gtgaaatacg gtgaatttct taacgaacag ccggaactga
     2221 aacgcctggc agagcagctg gggcgttctc gggaagccaa atcaataccg cgcaacgatg
     2281 cgcagatgga aaccttccgc accatggtgc gcgaaccggc gacggttcct gagcaggttg
     2341 atggtctgca acaaagcgat gatattttac gtctcctgcc gccagaactg gcgacactag
     2401 ggataacgga actggagtat gagttttacc gtcggctggt ggaaaaacag ttgctcacct
     2461 atcgcctgca cggtgagtcg tggcgtgaaa aagtgttcga acgtccggtg gtacataaag
     2521 attacgatga acagccgcgc gggccgttta ttgtctgtgt ggatacttcc ggctcaatgg
     2581 gcggctttaa tgaacagtgt gcgaaagcgt tctgcctggc tttgatgcgc attgctctcg
     2641 ctgaaaaccg gcgctgttat attatgctat tttccaccga gatcgtccgt tatgagcttt
     2701 caggcccaca aggcatcgaa caagcaatcc gttttttaag ccagcagttt cgtggcggca
     2761 ccgatcttgc cagttgtttt cgcgccatta tggaacgctt gcaaagcagg gaatggtttg
     2821 atgccgatgc ggtggtgatt tctgatttta tcgctcagcg gttgcctgac gacgtgacga
     2881 gtaaagtgaa agagctgcag cgggtacatc agcatcgctt tcatgccgtg gcgatgtcgg
     2941 cacacggcaa acccggcatc atgcgcattt tcgatcatat ctggcgcttt gataccggga
     3001 tgcgaagccg cctgctcaga cgctggcggc gataaattat tacagcagag aagggacgct
     3061 ctcgcgaaca gcagctggcc atactccaca ctgaacctgg ccgatatgcg gcagttgcag
     3121 cagcagcata gtcaaacgag actggccgat accgccgccg atggtctgcg gcatttcacc
     3181 gcgcagcagc gcctgatgcc attccagctg cagacgatct tcatcaccgg tcagcgccag
     3241 ctgatgcttc agcgtgtcgg catccacgcg gatccccatg gaagaaagct caaacgcatc
     3301 ttccagtacc gggttccaca ccagaatatc gccgttcagg cccgcatggc ccagctctga
     3361 cggggtgctc cagtcatcat aatccggtgc gcgcacgtcg tgacgatgac catcactcaa
     3421 cttgccgcca atcccgacga ggaataccgc gccaagatct ttcgcaatcg cccgctcacg
     3481 ccctttggca tcgagatccg gataacgaga cagtaactcc tggctgtgta cgaagtgaat
     3541 ctgatccggc aggaacggtg ccaggccaaa ctcttcgtta accgcagctt cggttgcttt
     3601 aattcccgcc cagatcgcct ctaccgtgct tttcagagtc gagagttgac gctcaccgtc
     3661 gcccattacg cgttcccagt cccactggtc aacatagacc gagtgcaacg gagaaagacg
     3721 gtcttcatcg gggcgaaggg ctttcatgtg cgtgtacagc ccttcgcccg cgctgaagtc
     3781 gtgttgccct aaggtctgac gtttccactt cgccagtgaa tgaaccactt cgaactgggc
     3841 atcaggcaga gctttcactt ttacctgcac cgctttttca cagcccgaca agttatcctg
     3901 cgtgccatcc cccacacggc taagaatcgg cgcctggact tcgatcagcc ccagacgttc
     3961 ttccagttgg cgagaaaagt gagatttcac gaagctaatt tggcgttgtt tggcaatgta
     4021 agcggttttc attttttata ctcctgcgtc ctgttgctta tgattaagca acaaaaaccg
     4081 acacacatgc aataatcatt caataaaaag cctaacacac ttttgattca ttaaaaaaga
     4141 cggctaaaat agaatgaatc atcaatctgc ataagaaaat cctatggaaa attatctgat
     4201 cgacaatctg gaccgtggca tcctggaagc attaatgggc aatgcgcgca ccgcttacgc
     4261 cgaactggcg aaacaatttg gcgtcagtcc ggggacgatt cacgttcgag tagagaaaat
     4321 gaagcaggcg gggatcatta ccggggcgcg tattgatgtc agcccgaagc agctcggtta
     4381 tgacgtaggc tgctttatcg gcattatatt aaagagcgcc aaagactacc cttccgcgct
     4441 ggcaaagctg gaaagccttg atgaagtcac tgaagcctat tacacaaccg gccactacag
     4501 catctttata aaagttatgt gccgttcgat cgacgctctc cagcatgtac ttatcaacaa
     4561 gatccaaaca attgatgaaa ttcagtccac cgagacattg atcgtcctgc aaaacccgat
     4621 catgcgtacc atcaagccct gatcggcttt tttaatccca tacttttcca caggtagatc
     4681 ccaacgcgtt cacagcgtac aatacgccac tcttaataaa ggtggcggtt tatggcagat
     4741 atcactctta tcagcggcag caccctcggc ggtgccgaat atgtagcaga acacctggct
     4801 gaaaagctgg aagaggtggg ttttaccacc gaaacgctgc acggtccgct gttggaagat
     4861 ttatctgcct cggggatctg gctggttatc agctccaccc acggtgccgg agatattccg
     4921 gacaaccttt ctcctttcta tgaagcattg caggaacaga agcccgatct ttctgcggtc
     4981 cgctttggca caatcggtat tggcagtcgt gaatatgaca ccttttgtgg ggctatcgat
     5041 aaactcgagg cagaactcaa aaattccggt gcaaaacaga caggcgaaac actgaagatc
     5101 aacattcttg atcacgacat tccggaagat ccggcagaag aatggctggg atcgtggatt
     5161 aatttactca aataagtata cagatcgtgc gatctactgt ggataactct gtcaggaagc
     5221 ttggatcaac cggtagctat ccaaagaaca accgttgttc agtttttgag ttgtgtataa
     5281 cccctcattc tgatcccagc ttatacggtc caggatcacc gatcattcac agttaatgat
     5341 cctttccagg ttgttgatct taaaaatcgg atccttgtta tccacagggc agtgcgatcc
     5401 taataagaga tcacaataga acagatctct aaataaatag atcttctttt taatacccag
     5461 gatcccaggt ctttctcaag ccgacaaagt tgagtagaat ccacggcccg ggcttcaatc
     5521 cattttcata ccgcgttatg cgaggcaatc accatgtttt atccggatcc ttttgacgtc
     5581 atcatcattg gcgggggtca tgcaggcacc gaggccgcga tggctgcggc gcgtatgggt
     5641 caacagactc tgcttttgac acacaatatc gacactctgg ggcagatgag ctgcaacccg
     5701 gcgatcggcg gtattgggaa gggacatctg gtaaaagaag tggatgcact cggcggtctg
     5761 atggcgaaag cgatcgatca agcgggtatc cagtttagga tactaaacgc aagcaaagga
     5821 ccggcggttc gcgctacccg agctcaggcg gatcgtgtgc gctaccgtca ggcggtacgt
     5881 acggcgctgg agaaccaacc gaacctgatg atcttccagc aggcggttga agatcttatt
     5941 gtcgaaaacg atcgcgtggt cggtgctgtt acccaaatgg gactgaagtt ccgtgccaaa
     6001 gctgtggtgc tcaccgttgg gacgttcctc gacggcaaaa ttcatatcgg tctggataat
     6061 tacagcggtg gccgtgctgg cgatccgcca tccattccgc tctctcgccg tttgcgtgaa
     6121 ctgccgctgc gcgttggtcg tctgaaaacc ggtactccgc cgcgtattga tgctcgaacc
     6181 atcgacttta gcgtgctggc gcaacagcat ggcgataacc caatgccggt attctcgttt
     6241 atgggcaatg cgtccctgca tccccagcag gtgccgtgtt atatcactca taccaacgag
     6301 aaaacccatg atgtgatccg cagtaacctc gatcgtagcc caatgtatgc aggggtgatc
     6361 gaaggtgtcg gcccacgcta ctgcccgtcg atcgaagaca aagtcatgcg cttcgcagac
     6421 agaaatcagc atcagatctt ccttgaaccg gaaggactga cctctaatga aatttatccg
     6481 aacggtatct ccaccagcct gccgttcgat gtgcagatgc aaatcgtccg ctctatgcag
     6541 gggatggaaa acgcgaagat cgtgcgtccg ggttatgcca ttgagtatga cttcttcgat
     6601 cctcgcgacc tgaaaccgac gctggagagc aagtttatcc aggggctgtt ctttgctggt
     6661 cagattaacg gcactaccgg ttacgaagaa gccgctgcgc aaggtttgct ggccggtctt
     6721 aacgctgccc gtctgtctgc tgacaaagaa ggttgggctc cggcacgttc tcaggcgtat
     6781 ctcggcgtac tggttgatga cctgtgcact ttaggaacca aagaaccgta tcgtatgttt
     6841 acttcgcgcg cagaatatcg tctgatgctg cgcgaagata atgcggatct gcgtttgact
     6901 gaaatcggtc gtgaactggg cctggtggat gacgaacgtt gggcgcgctt taacgagaaa
     6961 cttgagaata tcgagcgtga gcgtcagcgt ctgaaatcga cctgggtaac cccgtcggcg
     7021 gaagctgcag ccgaagtgaa tgctcacctg actgcgccgc tttcccgtga agccagtggt
     7081 gaagatctgc tgcgtcgtcc ggaaatgact tatgaaaaat taaccacgct gacgccgttt
     7141 gcccctgcgt tgacagacga acaggcggcg gaacaggttg agattcaggt taaatacgaa
     7201 ggttatatcg cgcgccagca agatgagatc gaaaagcagc tgcgtaacga gaacaccctg
     7261 ctacccgcga cactggatta ccgccagata tccggtcttt ctaacgaagt gatcgccaaa
     7321 cttaacgatc acaaaccggc ctctatcggc caggcttcgc gtatttctgg cgtcacgcct
     7381 gcggccatct ccattctgct ggtgtggctg aaaaaacagg gtatgctgcg tcgtagcgca
     7441 taacgcatta aaaatgcctg gtaagcaccc gcttaccagg caacgcatca agaacaggta
     7501 atcaccgtgc tcaacaaact ctccttactg ctgaaagacg caggtatttc gcttactgat
     7561 caccagaaaa accagcttat tgcctacgtg aatatgctgc ataaatggaa caaagcgtac
     7621 aacctgactt cggtccgcga tcctaatgag atgctggtac gccatattct cgatagcatt
     7681 gtggtggcac cgtatctgca aggtgaacgg tttatcgatg tcggcaccgg accaggactg
     7741 ccaggcattc cactctctat cgtgcgtcct gaagcccatt tcactctgtt ggatagcctt
     7801 ggtaaacgcg tgcgtttcct tcgtcaggtg caacatgagc ttaaactgga gaatattgaa
     7861 ccagtacaga gcagggtaga agagtttcct tcagagccgc catttgatgg cgtaattagc
     7921 cgcgcttttg cctctctgaa cgatatggtg agctggtgcc accatcttcc tggtgagcaa
     7981 ggccgtttct acgcgctgaa agggcaaatg ccggaagatg aaatcgcttt gttgcccgaa
     8041 gaatatcagg tcgaatcagt ggttaaactt caggttccag ccctggatgg cgaacgtcat
     8101 ctggtggtga ttaaagcaaa taaaatttaa tttttatcaa aaaaatcata aaaaattgac
     8161 cggttagact gttaacaaca accaggtttt ctactgatat aactggttac atttaacgcc
     8221 acgttcactc ttttgcatca acaagataac gtggcttttt ttggtaagca gaaaataagt
     8281 cattagtgaa aatatcagtc tgctaaaaat cggcgctaag aaccatcatt ggctgttaaa
     8341 acattattaa aaatgtcaat gagtggtttt tgttgtgtaa atgttattta ttaaaacagt
     8401 atctgttttt agactgaaat atcataaact tgtaaaggca tcatttgcca agtaaataaa
     8461 tatgctgtgc gcgaacatgc gcaatatgtg atctgaagca cgctttatca ccagtgttta
     8521 cgcgttattt acagtttttc atgatcgaac aggggtagcg gaaaagtcgc aattgtatgc
     8581 actggaaaaa tatttaaaca tttattcacc ttttggctac ttattgtttg aaatcacggg
     8641 ggcgcaccgt ataatttgac cgctttttga tgcttgactc taagccttaa agaaagtttt
     8701 atacgacacg cggcatacct cgaagggagc aggagtgaaa aacgtgatgt ctgtgtcgct
     8761 cgtgagtcga aacgttgctc ggaagcttct gctcgttcag ttactggtgg tgatagcaag
     8821 tggattgctg ttcagcctca aagacccctt ctggggcgtc tctgcaataa gcgggggcct
     8881 ggcagtcttt ctgcctaacg ttttgtttat gatatttgcc tggcgtcacc aggcgcatac
     8941 accagcgaaa ggccgggtgg cttggacatt cgcatttggc gaagctttca aagttctggc
     9001 gatgttggtg ttactggtgg tggcgttggc ggttttaaag gcggtattct tgccgctgat
     9061 cgttacgtgg gttttggtgc tggtggttca gatactggca ccggctgtaa ttaacaacaa
     9121 agggtaaaag gcatcatggc ttcagaaaat atgacgccgc aggattacat aggacaccac
     9181 ctgaataacc ttcagctgga cctgcgtaca ttctcgctgg tggatccaca aaacccccca
     9241 gccaccttct ggacaatcaa tattgactcc atgttcttct cggtggtgct gggtctgttg
     9301 ttcctggttt tattccgtag cgtagccaaa aaggcgacca gcggtgtgcc aggtaagttt
     9361 cagaccgcga ttgagctggt gatcggcttt gttaatggta gcgtgaaaga catgtaccat
     9421 ggcaaaagca agctgattgc tccgctggcc ctgacgatct tcgtctgggt attcctgatg
     9481 aatctgatgg atttactgcc tatcgacttg ctgccgtaca ttgctgaaca tgtactgggt
     9541 ctgcctgcac tgcgtgtggt tccgtctgcg gacgtgaacg taacgctgtc tatggcactg
     9601 ggcgtattta tcctgattct gttctacaac atcaaaatga aaggcatcgg cggcttcacg
     9661 aaagagttga cgctgcagcc gttcaatcac tgggcgttca ttcctgtcaa cttaatcctt
     9721 gaaggggtaa gcctgctgtc caaaccagtt tcactcggtt tgcgactgtt cggtaacatg
     9781 tatgccggtg agctgatttt cattctgatt gctggtctgt tgccgtggtg gtcacagtgg
     9841 atcctgaatg tgccgtgggc cattttccac atcctgatca ttacgctgca agccttcatc
     9901 ttcatggttc tgacgatcgt ctatctgtcg atggcgtctg aagaacatta atttaccaac
     9961 actactacgt tttaactgaa acaaactgga gactgtcatg gaaaacctga atatggatct
    10021 gctgtacatg gctgccgctg tgatgatggg tctggcggca atcggtgctg cgatcggtat
    10081 cggcatcctc gggggtaaat tcctggaagg cgcagcgcgt caacctgatc tgattcctct
    10141 gctgcgtact cagttcttta tcgttatggg tctggtggat gctatcccga tgatcgctgt
    10201 aggtctgggt ctgtacgtga tgttcgctgt cgcgtagtaa gcgttgcttt tatttaaaga
    10261 gcaatatcag aacgttaact aaatagaggc attgtgctgt gaatcttaac gcaacaatcc
    10321 tcggccaggc catcgcgttt gtcctgttcg ttctgttctg catgaagtac gtatggccgc
    10381 cattaatggc agccatcgaa aaacgtcaaa aagaaattgc tgacggcctt gcttccgcag
    10441 aacgagcaca taaggacctt gaccttgcaa aggccagcgc gaccgaccag ctgaaaaaag
    10501 cgaaagcgga agcccaggta atcatcgagc aggcgaacaa acgccgctcg cagattctgg
    10561 acgaagcgaa agctgaggca gaacaggaac gtactaaaat cgtggcccag gcgcaggcag
    10621 aaattgaagc cgagcgtaaa cgtgcccgtg aagagctgcg taagcaagtt gctatcctgg
    10681 ctgttgctgg cgccgagaag atcatcgaac gttccgtgga tgaagctgct aacagcgaca
    10741 tcgtggataa acttgtcgct gaactgtaag gagggagggg ctgatgtctg aatttattac
    10801 ggtagctcgc ccctacgcca aagcagcttt tgactttgcc gtcgaacacc aaagtgtaga
    10861 acgctggcag gacatgctga cgtttgccgc cgaggtaacc aaaaacgaac aaatggcaga
    10921 gcttctctct ggcgcgcttg cgccagaaac gctcgccgag tcgtttatcg cagtttgtgg
    10981 tgagcaactg gacgaaaacg gtcagaacct gattcgggta atggctgaaa atggtcgtct
    11041 taacgcgctc ccggatgttc tggagcagtt tattcacctt cgtgccgtga gtgaggctac
    11101 cgctgaggta gacgtcattt ccgctgccgc actgagtgaa caacagctcg cgaaaatttc
    11161 tgctgcgatg gaaaaacgtc tgtcacgcaa agttaagctg aattgcaaaa tcgataagtc
    11221 tgtaatggct ggcgttatca tccgatcggg tgatatggtc attgatggca gcgtacgcgg
    11281 tcgtcttgag cgccttgcag acgtcttgca gtcttaaggg gactggagca tgcaactgaa
    11341 ttccaccgaa atcagcgaac tgatcaagca gcgcattgct cagttcaatg ttgtgagtga
    11401 agctcacaac gaaggtacta ttgtttctgt aagtgacggt gttatccgca ttcacggcct
    11461 ggccgattgt atgcagggtg aaatgatctc cctgccgggt aaccgttacg ctatcgcact
    11521 gaacctcgag cgcgactctg taggtgcggt tgttatgggt ccgtacgctg accttgccga
    11581 aggcatgaaa gttaagtgta ctggccgtat cctggaagtt ccggttggcc gtggcctgct
    11641 gggccgtgtg gttaacactc tgggtgcacc aatcgacggt aaaggtccgc tggatcacga
    11701 cggcttctct gctgtagaag caatcgctcc gggcgttatc gaacgtcagt ccgtagatca
    11761 gccggtacag accggttata aagccgttga ctccatgatc ccaatcggtc gtggtcagcg
    11821 tgaattgatc atcggtgacc gtcagacagg taaaaccgca ctggctatcg atgccatcat
    11881 caaccagcgc gattccggta tcaaatgtat ctatgtcgct atcggccaga aagcgtccac
    11941 catttctaac gtggtacgta aactggaaga gcacggcgca ctggctaaca ccatcgttgt
    12001 ggtagcaacc gcgtctgaat ccgctgcact gcaatacctg gcaccgtatg ccggttgcgc
    12061 aatgggcgaa tacttccgtg accgcggtga agatgcgctg atcatttacg atgacctgtc
    12121 taaacaggct gttgcttacc gtcagatctc cctgctgctc cgtcgtccgc caggacgtga
    12181 agcattcccg ggcgacgttt tctacctcca ctctcgtctg ctggagcgtg ctgcacgtgt
    12241 taacgccgaa tacgttgaag ccttcaccaa aggtgaagtg aaagggaaaa ccggttctct
    12301 gaccgcactg ccgattatcg aaactcaggc gggtgacgtt tctgcgttcg ttccgaccaa
    12361 cgtaatctcc attaccgatg gtcagatctt cctggaaacc aacctgttca acgccggtat
    12421 tcgtcctgcg gttaacccgg gtatttccgt atcccgtgtt ggtggtgcag cacagaccaa
    12481 gatcatgaaa aaactgtccg gtggtatccg taccgctctg gcacagtatc gtgaactggc
    12541 agcgttctct cagtttgcat ccgaccttga cgatgcaaca cgtaagcagc ttgaccacgg
    12601 tcagaaagtg accgaactgc tgaaacagaa acagtatgcg ccgatgtccg ttgcgcagca
    12661 gtctctggtt ctgttcgcag cagaacgtgg ttacctggcg gatgttgaac tgtcgaaaat
    12721 tggcagcttc gaagccgctc tgctggctta cgtcgaccgt gatcacgctc cgttgatgca
    12781 agagatcaac cagaccggtg gctacaacga cgaaatcgaa ggcaagctga aaggcatcct
    12841 cgattccttc aaagcaaccc aatcctggta acgtctggag gcttgcctta gggcaggccg
    12901 caaggcattg aggagaagct catggccggc gcaaaagaga tacgtagtaa gatcgcaagc
    12961 gtccagaaca cgcaaaagat cactaaagcg atggagatgg tcgccgcttc caaaatgcgt
    13021 aaatcgcagg atcgcatggc ggccagccgt ccttatgcag aaaccatgcg caaagtgatt
    13081 ggtcaccttg cacacggtaa tctggaatat aagcaccctt acctggaaga ccgcgacgtt
    13141 aaacgcgtgg gctacctggt ggtgtcgacc gaccgtggtt tgtgcggtgg tttgaacatt
    13201 aacctgttca aaaaactgct ggcggaaatg aagacctgga ccgacaaagg cgttcaatgc
    13261 gacctcgcaa tgatcggctc gaaaggcgtg tcgttcttca actccgtggg cggcaatgtt
    13321 gttgcccagg tcaccggcat gggggataac ccttccctgt ccgaactgat cggtccggta
    13381 aaagtgatgt tgcaggccta cgacgaaggc cgtctggaca agctttacat tgtcagcaac
    13441 aaatttatta acaccatgtc tcaggttccg accatcagcc agctgctgcc gttaccggca
    13501 tcagatgatg atgatctgaa acataaatcc tgggattacc tgtacgaacc cgatccgaag
    13561 gcgttgctgg ataccctgct gcgtcgttat gtcgaatctc aggtttatca gggcgtggtt
    13621 gaaaacctgg ccagcgagca ggccgcccgt atggtggcga tgaaagccgc gaccgacaat
    13681 ggcggcagcc tgattaaaga gctgcagttg gtatacaaca aagctcgtca ggccagcatt
    13741 actcaggaac tcaccgagat cgtctcgggg gccgccgcgg tttaaacagg ttatttcgta
    13801 gaggatttaa gatggctact ggaaagattg tccaggtaat cggcgccgta gttgacgtcg
    13861 aattccctca ggatgccgta ccgcgcgtgt acgatgctct tgaggtgcaa aatggtaatg
    13921 agcgtctggt gctggaagtt cagcagcagc tcggcggcgg tatcgtgcgt accatcgcaa
    13981 tgggttcctc cgacggtctg cgtcgcggtc tggatgtaaa agacctcgaa cacccgatcg
    14041 aagtcccggt aggtaaagcg actctgggcc gtatcatgaa cgtactgggt gaaccggtcg
    14101 acatgaaagg cgagatcggt gaagaagagc gttgggcgat tcaccgcgca gcaccttcct
    14161 acgaagagct gtcaaactct caggaactgc tggaaaccgg tatcaaagtt atcgacctga
    14221 tgtgtccgtt cgctaagggc ggtaaagttg gtctgttcgg tggtgcgggt gtaggtaaaa
    14281 ccgtaaacat gatggagctc attcgtaaca tcgcgatcga gcactccggt tactctgtgt
    14341 ttgcgggcgt aggtgaacgt actcgtgagg gtaacgactt ctaccacgaa atgaccgact
    14401 ccaacgttat cgacaaagta tccctggtgt atggccagat gaacgagccg ccgggaaacc
    14461 gtctgcgcgt tgctctgacc ggtctgacca tggctgagaa attccgtgac gaaggtcgtg
    14521 acgttctgct gttcgttgac aacatctatc gttacaccct ggccggtacg gaagtatccg
    14581 cactgctggg ccgtatgcct tcagcggtag gttatcagcc gaccctggcg gaagagatgg
    14641 gcgttctgca ggaacgtatc acctccacca aaactggttc tatcacctcc gtacaggcgg
    14701 tatacgtacc tgcggatgac ttgactgacc cgtctccggc aaccaccttt gcgcaccttg
    14761 acgcaaccgt ggtactgagc cgtcagatcg cgtctctggg tatctacccg gccgttgacc
    14821 cgctggactc caccagccgt cagctggacc cgctggtggt tggtcaggaa cactacgaca
    14881 ccgcgcgtgg cgttcagtcc atcctgcaac gttatcagga actgaaagac atcatcgcca
    14941 tcctgggtat ggatgaactg tctgaagaag acaaactggt ggtagcgcgt gctcgtaaga
    15001 tccagcgctt cctgtcccag ccgttcttcg tggcagaagt attcaccggt tctccgggta
    15061 aatacgtctc cctgaaagac accatccgtg gctttaaagg catcatggaa ggcgaatacg
    15121 atcacctgcc ggagcaggcg ttctacatgg tcggttccat cgaagaagct gtggaaaaag
    15181 ccaaaaaact ttaacgcctt aatcggaggg tgatatggca atgacttacc acctggacgt
    15241 cgtcagcgca gagcaacaaa tgttctctgg tctggtcgag aaaatccagg taacgggtag
    15301 cgaaggtgaa ctggggatct accctggcca cgcaccgctg ctcaccgcca ttaagcctgg
    15361 tatgattcgc atcgtgaaac agcacggtca cgaagagttt atctatctgt ctggcggcat
    15421 tcttgaagtg cagcctggca acgtgaccgt tctggccgac accgcaattc gcggccagga
    15481 tctcgacgaa gcgcgagcca tggaagcgaa acgtaaggct gaagagcaca ttagcagctc
    15541 tcacggcgac gtagattacg ctcaggcgtc tgcggaactg gccaaagcga tcgcgcagct
    15601 gcgcgttatc gagttgacca aaaaagcgat gtaacaccgg cttgaaaagc acaaaagcca
    15661 gtctggaaac aggctggctt tttttacgcg ttgacccgtc ctgaagagcg ttcacataga
    15721 tcctgctgat ataaaacacc cctgttttcc cgtttattca ttgatcgaaa taagagcaaa
    15781 aacatccacc tgacgcttaa atgaaggtac tgccttaatt ttctgcagat aaaaggcgtg
    15841 acgatggtcg aaaatggcac tttcgtcagc ggggataatc cgttattgaa caatttatcc
    15901 tctgtccatt tcacgatgaa aaaaatgtag ttttttcaag gtgaagcggt ttaaattcgt
    15961 tctcaaatta cagtcaggac gcgtatgttg aataatgcta tgagcgtagt gatccttgcc
    16021 gcaggcaaag gcacgcgcat gtattccgat cttccgaaag tgctgcatac ccttgccggg
    16081 aaagcgatgg ttcagcatgt cattgatgct gcgaatgaat taggcgcagc gcacgttcac
    16141 ctggtgtacg gtcacggcgg cgatctgctt aaacaggcgc tgaaagacga caaccttaac
    16201 tgggtgcttc aggcagagca gctgggtacg ggtcatgcaa tgcagcaggc cgcacctttc
    16261 tttgccgatg atgaagacat tttaatgctc tacggcgacg tgccgctgat ctctgtcgaa
    16321 acactccagc gcctgcgtga tgctaaaccc cagggtggca ttggtctgct gacggtgaaa
    16381 ctggatgatc cgaccggtta tggacgtatc acccgtgaaa atggcaaagt taccggcatt
    16441 gttgagcaca aagatgccac cgacgagcag cgtcagattc aggagatcaa caccggcatt
    16501 ctgattgcca acggcgcaga tatgaaacgc tggctggcga agctgaccaa caataatgct
    16561 cagggcgaat actacatcac cgacattatt gcgctggcgt atcaggaagg gcgtgaaatc
    16621 gtcgccgttc atccgcaacg tttaagcgaa gtagaaggtg tgaataaccg cctgcaactc
    16681 tcccgtctgg agcgcgttta ccagtccgaa caggctgaaa aactgctgtt agcaggcgtt
    16741 atgctgcgcg atccggcgcg ttttgatctg cgcggtacgc ttactcacgg gcgcgatgtt
    16801 gaaattgata ctaacgttat catcgagggc aacgtgactc tcggtcatcg cgtgaaaatc
    16861 ggcaccggtt gcgtgattaa aaacagcgtg attggcgatg attgcgaaat cagtccgtat
    16921 accgttgtgg aagacgcgaa tctggcagcg gcctgtacta ttggcccgtt tgcccgtctg
    16981 cgtcctggtg ctgagttgct ggaaggtgca cacgttggta acttcgttga gatgaaaaaa
    17041 gcgcgtctgg gtaaaggcac gaaagccggg catctgactt acctgggcga tgcggaaatt
    17101 ggcgataacg ttaacatcgg cgcgggaacc attacctgca actacgatgg tgcgaataaa
    17161 tttaagacca ttatcggcga cgatgtgttt gtcggttccg acactcagct ggtggccccg
    17221 gtaacagtag gcaaaggcgc gaccattgct gcgggtacaa ctgtgacgcg taatgtcggc
    17281 gaaaatgcat tagctatcag ccgtgtgccg cagactcaga aagaaggctg gcgtcgtccg
    17341 gtaaagaaaa agtaattctg gccgggtaat ccggtcatct gggatgagga gataacataa
    17401 tctccctccc acaagcagta actataaaaa taaccccact ctctacaagg ctcggggcgc
    17461 ccgaaaaaac gggcatacag gttgaccgac aacgatataa atcggaatca aaaactatgt
    17521 gtggaattgt tggcgcgatc gcgcaacgtg atgtagcaga aatccttctt gaaggtttac
    17581 gtcgtctgga ataccgcgga tatgactctg ccggtctggc cgttgttgat gcggaaggtc
    17641 atatgactcg cctgcgtcgc ctcggtaaag tccagatgct ggctcaggca gcggaagaac
    17701 atcctctgca tggcggcacc ggtattgctc acactcgctg ggcgacccac ggtgaacctt
    17761 cagaagctaa cgcgcatccg catgtttctg aacacattgt ggtggtgcat aatggcatca
    17821 tcgaaaacca tgaaccgctg cgtgaagagt taaaagcgcg tggctatacc ttcgtttctg
    17881 aaaccgacac cgaagtgatt gcccatctgg tgaactggga gctgaaacaa ggcgggactc
    17941 tgcgtgaggc tgttctgcgt gctatcccgc agctgcgcgg tgcgtacggt acagtgatca
    18001 tggactcccg tcacccggat accctgctag cggcacgttc tggtagtccg ctggtgattg
    18061 gcctgggtat gggcgaaaac tttatcgctt ctgaccagct ggcgctgttg ccggtgaccc
    18121 gtcgctttat cttccttgaa gagggcgata ttgcggaaat cactcgccgt tcggtaaaca
    18181 tcttcgataa aactggcgcg gaagtaaaac gtcaggatat cgaatccaat ctgcaatatg
    18241 acgcgggcga taaaggtatt taccgtcact acatgcagaa agagatctac gaacagccga
    18301 acgcgataaa aaacaccctt accgggcgca tcagccacgg tcaggttgat ttaagcgagc
    18361 tgggaccgaa cgccgacgaa ctgctgtcga aggttgagca tattcagatc ctcgcctgtg
    18421 gtacttctta taactccggt atggtttccc gctactggtt tgaatcgctg gcaggtattc
    18481 cgtgcgacgt cgaaatcgcc tctgaattcc gctatcgtaa atctgccgtg cgtcgtaaca
    18541 gcctgatgat caccttgtca cagtctggcg aaaccgcgga taccctggcg ggcctgcgtc
    18601 tgtcgaaaga gctgggttac cttggttcgc tggcaatctg taacgttccg ggttcttctc
    18661 tggtgcgcga atccgatctg gcgctgatga ccaacgcggg tacggaaatc ggcgtggcgt
    18721 ccactaaagc attcaccact cagttaactg tgctgttgat gctggtggcg aagctgtctc
    18781 gcctgaaagg tctggatgcc tccattgaac atgacattgt gcatggtctg caggcgctgc
    18841 cgagccgtat tgagcagatg ctgtctcagg ataaacgcat tgaagctctg gcagaagatt
    18901 tctctgacaa acatcacgcg ctgttcctgg gccgtggcga tcagtaccca atcgcgctgg
    18961 aaggcgcatt gaagctgaaa gagatctctt acattcacgc tgaagcctac gctgcaggcg
    19021 agctgaaaca cggcccgctg gcgctgattg acgcggatat gccggttatc gtcgttgcac
    19081 cgaacaacga actgttggaa aaactgaaat ccaacattga agaagttcgc gcgcgtggcg
    19141 gtcaattgta tgtcttcgcc gatcaggatg cgggttttgt aagtagcgat aacatgcaca
    19201 tcatcgagat gccgcatgtg gaagaggtga ttgcaccgat cttctacacc gttccgctgc
    19261 agctgctggc ttaccatgtc gcgctgatca aaggcaccga cgttgaccag ccgcgtaacc
    19321 tggcaaaatc ggttacggtt gagtaatcac tatcatagat aaaaaagggc tgctgtgggc
    19381 ggacaataaa gtcttaaact gaacaaaata gatctaaact atgacaataa agtcttaaac
    19441 tagacagaat agttgtaaac tgaaatcagt ccagttatgc tgtgaaaaag catactggac
    19501 ttttgttatg gctaaagcaa actcttcatt ttctgaagtg caaattgccc gtcgtattaa
    19561 agaggggcgt ggccaagggc atggtaaaga ctatattcca tggctaacag tacaagaagt
    19621 tccttcttca ggtcgttccc accgtattta ttctcataag acgggacgag tccatcattt
    19681 gctatctgac ttagagcttg ctgtttttct cagtcttgag tgggagagca gcgtgctaga
    19741 tatacgcgag cagttcccct tattacctag tgataccagg cagattgcaa tagatagtgg
    19801 tattaagcat cctgttattc gtggtgtaga tcaggttatg tctactgatt ttttagtgga
    19861 ctgcaaagat ggtccttttg agcagtttgc tattcaagtc aaacctgcag cagccttaca
    19921 agacgagcgt accttagaaa aactagaact agagcgtcgc tattggcagc aaaagcaaat
    19981 tccttggttc atttttactg ataaagaaat aaatcccgta gtaaaagaaa atattgaatg
    20041 gctttattca gtgaaaacag aagaagtttc tgcggagctt ttagcacaac tatccccatt
    20101 ggcccatatc ctgcaagaaa aaggagatga aaacattatc aatgtctgta agcaggttga
    20161 tattgcttat gatttggagt taggcaaaac attgagtgag atacgagcct taaccgcaaa
    20221 tggttttatt aagttcaata tttataagtc tttcagggca aataagtgtg cagatctctg
    20281 tattagccaa gtagtgaata tggaggagtt gcgctatgtg gcaaattaat gaggttgtgc
    20341 tatttgataa tgatccgtat cgcattttgg ctatagagga tggccaagtt gtctggatgc
    20401 aaataagcgc tgataaagga gttccacaag ctagggctga gttgttgcta atgcagtatt
    20461 tagatgaagg ccgcttagtt agaactgatg acccttatgt acatcttgat ttagaagagc
    20521 cgtctgtaga ttctgtcagc ttccagaagc gcgaggagga ttatcgaaaa attcttccta
    20581 ttattaatag taaggatcgt ttcgacccta aagtcagaag cgaactcgtt gagcatgtgg
    20641 tccaagaaca taaggttact aaggctacag tttataagtt gttacgccgt tactggcagc
    20701 gtggtcaaac gcctaatgca ttaattcctg actacaaaaa cagcggtgca ccaggggaaa
    20761 gacgttcagc gacaggaaca gcaaagattg gccgagccag agaatatggt aagggtgaag
    20821 gaaccaaggt aacgcccgag attgaacgcc tttttaggtt gaccatagaa aagcacctgt
    20881 taaatcaaaa aggtacaaag accaccgttg cctatagacg atttgtggac ttgtttgctc
    20941 agtattttcc tcgcattccc caagaggatt acccaacact acgtcagttt cgttattttt
    21001 atgatcgaga ataccctaaa gctcagcgct taaagtctag agttaaagca ggggtatata
    21061 aaaaagacgt acgaccctta agtagtacag ccacttctca ggcgttaggc cctgggagtc
    21121 gttatgagat tgatgccacg attgctgata tttatttagt ggatcatcat gatcgccaaa
    21181 aaatcatagg aagaccaacg ctttacattg tgattgatgt gtttagtcgg atgatcacgg
    21241 gcttttatat cggctttgaa aatccgtctt atgtggtggc gatgcaggct tttgtaaatg
    21301 cttgctctga caaaacggcc atttgtgccc agcatgatat tgagattagt agctcagact
    21361 ggccgtgtgt aggtttgcca gatgtgttgc tagcggaccg tggcgaatta atgagtcatc
    21421 aggtcgaagc cttagtttct agttttaatg tgcgagtgga aagtgctcca cctagacgtg
    21481 gcgatgctaa aggcatagtg gaaagcactt ttagaacact acaagccgag tttaagtcct
    21541 ttgcacctgg cattgtagag ggcagtcgga tcaaaagcca tggtgaaaca gactataggt
    21601 tagatgcatc tctgtcggta tttgagttca cacaaattat tttgcgtacg atcttattca
    21661 gaaataacca tctggtgatg gataaatacg atcgagatgc tgattttcct acagatttac
    21721 cgtctattcc tgtccagcta tggcaatggg gtatgcagca tcgtacaggt agtttaaggg
    21781 ctgtggagca agagcagttg cgagtagcgt tactgcctcg ccgaaaggtc tctatttctt
    21841 catttggcgt taatttgtgg ggtttgtatt actcggggtc agagattctg cgtgagggtt
    21901 ggttgcagcg gagcactgat atagctagac ctcaacattt agaagcggct tatgacccag
    21961 tgctggttga tacgatttat ttgtttccgc aagttggcag ccgtgtattt tggcgctgta
    22021 atctgacgga acgtagtcgg cagtttaaag gtctctcatt ttgggaggtt tgggatatac
    22081 aagcacaaga aaaacacaat aaagccaatg cgaagcagga tgagttaact aaacgcaggg
    22141 agcttgaggc gtttattcag caaaccattc agaaagcgaa taagttaacg cccagtacta
    22201 ctgagcccaa atcaacacgc attaagcaga ttaaaactaa taaaaaagaa gccgtgacct
    22261 cggagcgtaa aaaacgtgcg gagcatttga agccaagctc ttcaggtgat gaggctaaag
    22321 ttattccttt caacgcagtg gaagcggatg atcaagaaga ttacagccta cccacatacg
    22381 tgcctgaatt atttcaggat ccaccagaaa aggatgagtc atgagtgcta cccggattca
    22441 agcagtttat cgtgatacgg gggtagaggc ttatcgtgat aatcctttta tcgaggcctt
    22501 accaccatta caagagtcag tgaatagtgc tgcatcactg aaatcctctt tacagcttac
    22561 ttcctctgac ttgcaaaagt cccgtgttat cagagctcat accatttgtc gtattccaga
    22621 tgactatttt cagccattag gtacgcattt gctactaagt gagcgtattt cggtcatgat
    22681 tcgaggtggc tacgtaggca gaaatcctaa aacaggagat ttacaaaagc atttacaaaa
    22741 tggttatgag cgtgttcaaa cgggagagtt ggagacattt cgctttgagg aggcacgatc
    22801 tacggcacaa agcttattgt taattggttg ttctggtagt gggaagacga cctctcttca
    22861 tcgtattcta gccacgtatc ctcaggtgat ttaccatcgt gaactcaatg tagagcaggt
    22921 ggtgtatttg aaaatagact gctcgcataa tggttcgcta aaagaaatct gcttgaattt
    22981 tttcagagcg ttggatcgag ccttgggctc gaactatgag cgtcgttatg gcttaaaacg
    23041 tcatggtata gaaaccatgt tggctttgat gtcgcaaata gccaatgcac atgctttagg
    23101 gttgttggtt attgatgaaa ttcagcattt aagccgctct cgttcgggtg gatctcaaga
    23161 gatgctgaac ttttttgtga cgatggtgaa tattattggc gtaccagtga tgttgattgg
    23221 tacccctaaa gcacgagaga tttttgaggc tgatttgcgg tctgcacgta gaggggcagg
    23281 gtttggagct atattctggg atcctataca acaaacgcaa cgtggaaagc ccaatcaaga
    23341 gtggatcgct tttacggata atctttggca attacagctt ttacaacgca aagatgcgct
    23401 gttatcggat gaggtccgtg atgtgtggta tgagctaagc caaggagtga tggacattgt
    23461 agtaaaactt tttgtactcg ctcagctccg tgcgctagct ttaggcaatg agcgtattac
    23521 cgctggttta ttgcggcaag tgtatcaaga tgagttaaag cctgtgcacc ccatgctaga
    23581 ggcattacgc tcgggtatcc cagaacgcat tgctcgttat tctgatctag tcgttcccga
    23641 gattgataaa cggttaatcc aacttcagct agatatcgca gcgatacaag aacaaacacc
    23701 agaagaaaaa gcccttcaag agttagatac cgaagatcag cgtcatttat atctgatgct
    23761 gaaagaggat tacgattcaa gcctgttaat tcccactatt aaaaaagcgt ttagccagaa
    23821 tccaacgatg acaagacaaa agttactgcc tcttgttttg cagtggttga tggaaggcga
    23881 aacggtagtg tcagaactag aaaagccctc caagagtaaa aaggtttcgg ctataaaggt
    23941 agtcaagccc agcgactggg atagcttgcc tgatacggat ttacgttata tctattcaca
    24001 acgccaacct gaaaaaacca tgcatgaacg gttaaaaggg aaaggggtaa tagtggatat
    24061 ggcgagctta tttaaacaag caggttagcc atgagaaact ttcctgttcc gtactcgaat
    24121 gagctgattt atagcactat tgcacgggca ggcgtttatc aagggattgt tagtcctaag
    24181 cagctgttgg atgaggtgta tggcaaccgc aaggtggtcg ctaccttagg tctgccctcg
    24241 catttaggtg tgatagcaag acatctacat caaacaggac gttacgctgt tcagcagctt
    24301 atttatgagc ataccttatt ccctttatat gctccgtttg taggcaagga gcgccgagac
    24361 gaagctattc ggttaatgga gtaccaagcg caaggtgcgg tgcatttaat gctaggagtc
    24421 gctgcttcta gagttaagag cgataaccgc tttagatact gccctgattg cgttgctctt
    24481 cagctaaata ggtatgggga agccttttgg caacgagatt ggtatttgcc cgctttgcca
    24541 tattgtccaa aacacggtgc tttagtcttc tttgatagag ctgtagatga tcaccgacat
    24601 caattttggg ctttgggtca tactgagctg ctttcagact accccaaaga ctccctatct
    24661 caattaacag cactagctgc ttatatagcc cctctgttag atgctccacg agcgcaagag
    24721 ctttccccaa gccttgagca gtggacgctg ttttatcagc gcttagcgca ggatctaggg
    24781 ctaaccaaaa gcaagcacat tcgtcatgac ttggtggcgg agagagtgag gcagactttt
    24841 agtgatgagg cactagagaa actggattta aagttggcag agaacaagga cacgtgttgg
    24901 ctgaaaagta tattccgtaa gcatagaaaa gcctttagtt atttacagca tagtattgtg
    24961 tggcaagcct tattgccaaa actaacggtt atagaagcgc tacagcaggc aagtgctctt
    25021 actgagcact ctataacgac aagacctgtt agccagtctg tgcaacctaa ctctgaagat
    25081 ttatctgtta agcataaaga ctggcagcaa ctagtgcata aataccaagg aattaaggcg
    25141 gcaagacagt ctttagaggg tggggtgcta tacgcttggc tttaccgaca tgacagggat
    25201 tggctagttc actggaatca acagcatcaa caagagcgtc tggcacccgc ccctagagtt
    25261 gattggaacc aaagagatcg aattgctgta cgacaactat taagaatcat aaagcgtcta
    25321 gatagtagcc ttgatcaccc aagagcgaca tcgagctggc tgttaaagca aactcctaac
    25381 ggaacctctc ttgcaaaaaa tctacagaaa ctgcctttgg tagcgctttg cttaaagcgt
    25441 tactcagaga gtgtggaaga ttatcaaatt agacggatta gccaagcttt tattaagctt
    25501 aaacaggaag atgttgagct taggcgctgg cgattattaa gaagtgcaac gttatctaaa
    25561 gagcggataa ctgaggaagc acaaagattc ttggaaatgg tttatgggga agagtgagtg
    25621 gttaggctag ctacatttaa tgacaatgtg caggttgtac atattggtca tttattccgt
    25681 aactcgggtc ataaggagtg gcgtattttt gtttggttta atccaatgca agaacggaaa
    25741 tggactcgat ttactcattt gcctttatta agtcgagcta aggtggttaa cagtacaaca
    25801 aagcaaataa ataaggcgga tcgtgtgatt gagtttgaag catcggatct tcaacgagcc
    25861 aaaataatcg attttcctaa tctctcgtcc tttgcttccg tacgcaacaa ggatggagcg
    25921 cagagttcat ttatttacga agctgaaaca ccatatagca agactcgtta tcacatccca
    25981 cagttagagc tagctcggtc attattttta attaactcct atttctgtcg aagctgtttg
    26041 agcagtaccg ctttacagca agagttcgac gttcagtatg aggttgagcg agatcattta
    26101 gagataagga tcttacccag ttcatcgttt cctaaagggg cgttagagct gtcggccgta
    26161 gtgcagcttt tggtttggtt gttttcggat caagatgtta tggattcgta tgaaagtatt
    26221 tttaggcact atcaacaaaa tagagaaatt aagaacggcg ttgaaagctg gtgctttagc
    26281 tttgaccctc cgcccatgca gggttggaaa ttacatgtaa aaggacgttc ttctaacgag
    26341 gataaggatt atttagttga ggaaatagta ggtttagaaa tcaacgctat gcttcctagc
    26401 acaacagcta ttagccatgc ctcttttcag gaaaaggagg caggtgatgg tagtacgcag
    26461 cacatagcgg tttcaacaga gtcagttgtt gatgatgagc atctacagtt ggacgatgag
    26521 gaaacagcca atatagacac agacacacga gtcatagagg ctgagccgac atggataagt
    26581 tttagtagac ctagtcgaat tgaaaaatct cgcagggcaa gaaaaagtag ccaaactatt
    26641 ttagaaaaag aagaagcaac aacaagtgaa aatagtaatt tggttagtac tgatgagcca
    26701 cacttaggtg gtgtcctagc agcggcagat gtgggtggga agcaggatgc aaccaattac
    26761 aactctattt ttgctaatcg atttgctgct tttgatgagc tactttcaat tctaaaaact
    26821 aaatttgcat gtcgggtgct ttttgaagaa accttggttt tgccaaaagt tgggcgtagc
    26881 cgattacatc tgtgtaaaga tggctcacca agagtgatta aagccgttgg ggtgcaacgt
    26941 aatggcagtg aatttgtatt gctagaggtg gatgcatcgg atggggtgaa aatgctttct
    27001 accaaagtgt tgagtggcgt tgatagcgaa acatggcgga atgattttga aaagatacgg
    27061 cgtggagtgg tgaagagctc attgaattgg ccaaatagtt tgtttgatca attatatgga
    27121 caagacgggc atagaggggt gaatcatcca aaggggttgg gggagctgca agtatcgaga
    27181 gaggatatgg aagggtgggc tgagagagtg gttagagagc aatttacgca ttaaaggaat
    27241 gactgaaaga gcctgtaaac ccttttgtgt aagtgctttt gccggtcagt taaaggtggc
    27301 catttaaacg gtcaccaaat tcgatcataa aacggttcat ggccggcttc cagttgcgga
    27361 tcggcatcgt ccatttcttg gtcgccgcct ggatagccag gtacaccacc ttcatcgctg
    27421 attcgtccgt agggaacacc ttgagttcat attaatggaa ttttctacaa atagcctccg
    27481 tggttttgag gggggattac agacgatcca tagtagtaat ccaatgagtt cttgagcgcg
    27541 gcgacatgtt tggacgcctt ggcaaaaatt agagcctgct tgaagtgcag gcgagcacgt
    27601 gcttggacga ttgatccatt cgcggtcaaa aactcaatct tggatgacag cgtgtcagga
    27661 aatccaatat cgaagtcccg ccccgtgtca aagtagggca tttcatgatc aaaggacgaa
    27721 gcttcccagg ccacttccct aatattccag ccgctagaaa caagatttcg tacgacttca
    27781 tcaacttttt tgctttccaa cttgtcacaa agtgcatagg caagagcttt ggcggcagac
    27841 acgtgcgttt cctcttaaac ataggtcgaa cggatacccg aggcaaagcg gagcattagt
    27901 cccaaagcat ctccggtaga tccggaggct ccatactgaa ggcatggcat cttgggcatt
    27961 gatatttgaa acttctctct agatagcagg gatttcttgg ccttgcctca tgctgatagc
    28021 ttgtaaatat cacacgatcc ttagccccac aaagtgagca tactccgtag ctttcattta
    28081 tagaaatcgt cgttagattg tcgcagcatg tgcacgttcc gactatttgc tcaggaagca
    28141 tcgccatacc ttggcctagg taaagaagat cagactcgta gccacatcgg tggcaactga
    28201 gcttgtacgc aatgcccatc tggttgtctc acctgaatcg taacgtcaga gctattcatc
    28261 catccattcg gcatcaatgt atgccactaa gccgaccagc tgaccgtttg gactgaagta
    28321 tgttgcctgc accccacttg acatctcatc aataccaccg cactgcgcta ggcgccggtc
    28381 ccacttgccc acagaagcgt caggatcggc tgagttcccc aagacccttg tgagccatgc
    28441 ctcctttaac tcaacctctc gaatgattgc gtcggccagc tcctccgcgt tatctagttc
    28501 gacatagtct ggaattcgga cgtacgttct gaacacagct ggatacttac ttgggcgatt
    28561 gtcatacatg acatcaacaa tgtacccgtt tgtgtaaccg tctcttggag gttcgtatga
    28621 cactagtggt tcccctcagc ttgcgactag atgttgaggc ctaacatttt attagagagc
    28681 aggctagttg cttagataca tgatcttcag gccgttatct gtcagggcaa gcgaaaattg
    28741 gccatttatg acgaccaatg ccccgcagaa gctcccatct ttgccgccat agacgccgcg
    28801 cccccctttt ggggtgtaga acatcctttt gccagatgtg gaaaagaagt tcgttgtccc
    28861 attgttggca atgacgtagt agccggcgaa agtgcgagac ccatttgcgc tatatataag
    28921 cctacgattt ccgttgcgac tattgtcgta attggatgaa ctattatcgt agttgctctc
    28981 agagttgtcg taatttgatg gactattgtc gtaattgctt atggagttgt cgtagttgct
    29041 tggagaaatg tcgtagttgg atggggagta gtcataggga agacgagctt catccactaa
    29101 aacaattggc aggtcagcaa gtgcctgccc cgatgccatc gcaagtacga ggcttagaac
    29161 caccttcaac agatcgcgca tagtcttccc cagctctcta acgcttgagt taagccgcgc
    29221 cgcgaagcgg cgtcggcttg aacgaattgt tagacattat ttgccgacta ccttggtgat
    29281 ctcgcctttc acgtagtgaa caaattcttc caactgatct gcgcgcgagg ccaagcgatc
    29341 ttcttgtcca agataagcct gcctagcttc aagtatgacg ggctgatact gggccggcag
    29401 gcgctccatt gcccagtcgg cagcgacatc cttcggcgcg attttgccgg ttactgcgct
    29461 gtaccaaatg cgggacaacg taagcactac atttcgctca tcgccagccc agtcgggcgg
    29521 cgagttccat agcgttaagg tttcatttag cgcctcaaat agatcctgtt caggaaccgg
    29581 atcaaagagt tcctccgccg ctggacctac caaggcaacg ctatgttctc ttgcttttgt
    29641 cagcaagata gccagatcaa tgtcgatcgt ggctggctcg aagatacctg caagaatgtc
    29701 attgcgctgc cattctccaa attgcagttc gcgcttagct ggataacgcc acggaatgat
    29761 gtcgtcgtgc acaacaatgg tgacttctac agcgcggaga atctcgctct ctccagggga
    29821 agccgaagtt tccaaaaggt cgttgatcaa agctcgccgc gttgtttcat caagccttac
    29881 ggtcaccgta accagcaaat caatatcact gtgtggcttc aggccgccat ccactgcgga
    29941 gccgtacaaa tgtacggcca gcaacgtcgg ttcgagatgg cgctcgatga cgccaactac
    30001 ctctgatagt tgagtcgata cttcggcgat caccgcttcc ctcatgatgt ttaactcctg
    30061 aattaagccg cgccgcgaag cggtgtcggc ttgaatgaat tgttaggcgt catcctgtgc
    30121 tcccgagaac cagtaccagt acatcgctgt ttcgttcgag acttgaggtc tagttttata
    30181 cgtgaacagg tcaatgccgc cgagagtaaa gccacatttt gcgtacaaat tgcaggcagg
    30241 tacattgttc gtttgtgtct ctaatcgtat gccaaggagc tgtctgctta gtgcccactt
    30301 tttcgcaaat tcgatgagac tgtgcgcgac tcctttgcct cggtgcgtgt gcgacacaac
    30361 aatgtgttcg atagaggcta gatcgttcca tgttgagttg agttcaatct tcccgacaag
    30421 ctcttggtcg atgaatgcgc catagcaagc agagtcttca tcagagtcat catccgagat
    30481 gtaatccttc cggtaggggc tcacacttct ggtagatagt tcaaagcctt ggtcggatag
    30541 gtgcacatcg aacacttcac gaacaatgaa atggttctca gcatccaatg tttccgccac
    30601 ctgctcaggg atcaccgaaa tcttcatatg acgcctaacg cctggcacag cggatcgcaa
    30661 acctggcgcg gcttttggta caaaaggcgt gacaggtttg cgaatccgtt gctgccactt
    30721 gttaaccctt ttgccagatt tggtaactat aatttatgtt agaggcgaag tcttgggtaa
    30781 aaactggcct aaaattgctg gggatttcag gaaagtaaac atcaccttcc ggctcgatgt
    30841 ctattgtaga tatatgtagt gtatctactt gatcgatcag gcttttgtat atctccccac
    30901 cacctgaaac aatgacatga tccgttattt tctttaggtt ggttaaagca tctttaattg
    30961 atggaaagat caatacgttc tcattgtcag atgtaaaact tgaacgtgtt acgaccgcat
    31021 actttcggtt gggtaatgct cccattgatt caaaagtctt gcgtccaacc aacagccatt
    31081 ggttataggt aatagcttta aacaggagct gttcaccttt ggcactccat ggaatatcag
    31141 ggccattccc gataactcca ttcttcgata tagctaccat tagtgatagt ttcacaattc
    31201 ttcctcagag gttaacattt tattagtaag catgctcgtt ttgtcacccg ctgatgctta
    31261 ccgttaatta atataatcac ttgtattaaa tagactttct ttaaaaatac aagacactct
    31321 gttattacaa atcgtgcatg ccgtctatcc tgcttgcacg atgcacatta tctcacccaa
    31381 agaactcata acattttaat ttatttagca ttaaattaaa actctaccgg taaacaagca
    31441 tctctaggcg taaaaaacag gcactttttt aaatcccagc tagaataggc tgtataggca
    31501 gacagttgca agacaaggag cgttttatgt ctaacagtcc atttttaaat tctatacgca
    31561 cggatatgcg acaaaaaggt tatgcgctga aaactgaaaa aacttacctg cactggatta
    31621 agcgttttat tctgtttcac aaaaaacgtc atcctcagac catgggcagt gaagaggtca
    31681 ggctgttttt atccagctta gcaaacagca gacatgtagc cataaacacg cagaaaatcg
    31741 ctttaaatgc cctagctttt ttgtacaaca ggtttttaca acagccgttg ggcgatattg
    31801 attatatccc tgcaagcaag cctagacggc taccctctgt tatctctgca aatgaagtgc
    31861 aacgcatttt gcaggttatg gatactcgca accaagttat ttttacgctg ctgtatggtg
    31921 caggtttgcg cattaatgaa tgcttgcgtt tgcgggttaa agattttgat tttgataatg
    31981 gctgcatcac tgtgcatgac ggtaagggtg ggaaaagcag aaacagccta ctgcccacgc
    32041 gcctaatccc agcaataaaa taactcattg agcaagcgcg gcttattcag caagacgaca
    32101 acttacaagg cgtagggcca tcgctgcctt ttgctttaga tcacaaatac ccttctgctt
    32161 atcgacaagc ggcgtggatg tttgtctttc cctccagcac gctctgcaac cacccgtata
    32221 acggcaaatt atgccgccat catctgcatg actccgttgc gcgaaaggca ttgaaggcag
    32281 ccgtacaaaa agcaggcatc gttagcaagc gtgtcacttg tcatacattt cgtcactcgt
    32341 ttgctacgca tctattacaa gcggggcgtg atattcgcac tgtgcaagaa ctcttagggc
    32401 ataacgatgt taagaccacg caaatctata cgcatgtgtt gggtcagcat tttgccggca
    32461 ccaccagtcc tgcggatgga ctgatgctac ttatcaatca gtaaaagtag ataaatatct
    32521 ttatgtagcc ctgagtagcc cctgatactt tttatggtga aaaagataaa caatcataga
    32581 ggggataaat atgagccagt tctaccattg ttcagataag tttaagcaag atactgtcaa
    32641 tcacagtgac cattcatggt cattacactt cctccagttg ccagacaatg cctaacataa
    32701 aaacgcaaaa gcccaaaagt cagagcttag gtgttctagg cgttttctaa gcgctgaaga
    32761 aatacgtctt accgtagatt gcgtgcgttt ctgaagcaca ttttccttat acatggcttt
    32821 atcccaggca gcttcatcaa gctcttggag taacaagcca gtaataatgc gacactctcg
    32881 ggtttgtagc gagccgctgg tgagatcgga gttatagcgg aaaggggtca tttgtaatta
    32941 tttgtgggtg gaggatagtt aacttattat tgtagttgga agaggctgct ttccaaacat
    33001 tagcacctcg taatgcttgt tactgtatat taatttatgc tcttagcgca tgttaatggg
    33061 ctattacaat gcataatgtc cacatcaaga taactgtggc ttaacacctg tacaagcgaa
    33121 aaaaatcaat cacctgtccg gaaatacggg atcactgtaa agtgttggtg aggcagtcta
    33181 ggagggggat ttaatttggg atactgatga aaggattatg atcagttgga tataggatcg
    33241 tactaagctg agcggcaggt tagctagatt agttaaccag ataagtgaaa tctagttcca
    33301 aactattttg tcatttttaa ttttcgtatt agcttacgac gctacaccca gttcccatct
    33361 attttgtcac tcttccctaa ataatcctta aaaactccat ttccacccct cccagttccc
    33421 aactattttg tccgcccaca gctgccg
//
LOCUS       DAJTRC010000040        33141 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.040, whole genome shotgun sequence.
ACCESSION   DAJTRC010000040 DAJTRC010000000
VERSION     DAJTRC010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 33141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.040"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(2..94)
                     /locus_tag="OO357_001991"
     tRNA            complement(2..94)
                     /locus_tag="OO357_001991"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(58..60),aa:Ser,seq:gct)
     gene            complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO357_001992"
     CDS             complement(410..595)
                     /gene="csrA"
                     /locus_tag="OO357_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001774971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon storage regulator CsrA"
                     /protein_id="HCR5886949.1"
                     /translation="MLILTRRVGETLMIGDEVTVTVLGVKGNQVRIGVNAPKEVSVHR
                     EEIYQRIQAEKSQQSSY"
     gene            complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO357_001993"
     CDS             complement(830..3460)
                     /gene="alaS"
                     /locus_tag="OO357_001993"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HCR5886950.1"
                     /translation="MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPHNDPTLLFTNAGM
                     NQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDY
                     FKHDAIQFAWELLTSEKWFALPKERLWVTVYESDDEAYEIWEKEVGIPRERIIRIGDN
                     KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQ
                     FNRQADGTMEPLPKPSVDTGMGLERIAAVLQHVNSNYDIDLFRTLIQAVAKVTGATDL
                     SNKSLRVIADHIRSCAFLIADGVMPSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKL
                     VGPLIDVMGSAGEDLKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLSGDTLDG
                     ETAFRLYDTYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMI
                     RVDSASEFKGYDHLELNGKVTALFVDGKAVDAINAGQEAVVVLDQTPFYAESGGQVGD
                     KGELKGANFSFAVEDTQKYGQAIGHIGKLAAGSLKVGDAVQADIDEARRARIRLNHSA
                     THLMHAALRQVLGTHVSQKGSLVNDKVLRFDFSHNEAMKPEEIRAVEDLVNAQIRRNL
                     PIETNIMNLEAAKAKGAMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRI
                     ISESGTAAGVRRIEAVTGEGAITTVHADSDRLSEVAHLLKGDSNNLADKVRSVLERTR
                     QLEKELQQLKEQAAAQESANLSSKAIDVNGVKLLVSELSGVEPKMLRTMVDDLKNQLG
                     STIIVLATVAEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTD
                     AAALPAALASVKGWVSAKLQ"
     gene            complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO357_001994"
     CDS             complement(3588..4088)
                     /gene="recX"
                     /locus_tag="OO357_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HCR5886951.1"
                     /translation="MTESTSRRPAYARLLDRAVRILAVRDHSEQELRRKLAAPIMGKN
                     GPEEIDATAEDYERVIAWCHEHGYLDDSRFVARFIASRSRKGYGPARIRQELNQKGIS
                     REATEKAMRECDIDWCALARDQATRKYGEPLPTVFSEKVKIQRFLLYRGYLMEDIQDI
                     WRNFAD"
     gene            complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO357_001995"
     CDS             complement(4331..5392)
                     /gene="recA"
                     /locus_tag="OO357_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HCR5886952.1"
                     /translation="MAIDENKQKALAAALGQIEKQFGKGSIMRLGEDRSMDVETISTG
                     SLSLDIALGAGGLPMGRIVEIYGPESSGKTTLTLQVIAAAQREGKTCAFIDAEHALDP
                     IYARKLGVDIDNLLCSQPDTGEQALEICDALARSGAVDVIVVDSVAALTPKAEIEGEI
                     GDSHMGLAARMMSQAMRKLAGNLKQSNTLLIFINQIRMKIGVMFGNPETTTGGNALKF
                     YASVRLDIRRIGAVKEGENVVGSETRVKVVKNKIAAPFKQAEFQILYGEGINFYGELV
                     DLGVKEKLIEKAGAWYSYKGEKIGQGKANATAWLKDNPETAKEIEKKVRELLLSNPNS
                     TPDFSVDDSEGVAETNEDF"
     gene            complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO357_001996"
     CDS             complement(5472..5969)
                     /gene="pncC"
                     /locus_tag="OO357_001996"
                     /EC_number="3.5.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide-nucleotide amidase"
                     /protein_id="HCR5886953.1"
                     /translation="MTDSELMQLSEQVGQALKARGATVTTAESCTGGWVAKVITDIAG
                     SSAWFERGFVTYSNEAKAQMIGVREETLAQHGAVSEPVVVEMAIGALKAARADYAVSI
                     SGIAGPDGGSEEKPVGTVWFAFATARGEGITRRECFSGDRDAVRRQATAYALQTLWQQ
                     FLQNT"
     gene            complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO357_001997"
     CDS             complement(6114..7199)
                     /gene="mltB"
                     /locus_tag="OO357_001997"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708509.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase B"
                     /protein_id="HCR5886954.1"
                     /translation="MFKRRYVTLLPLFVLLAACSSKPKPTETETTTGTPSGGFLLEPQ
                     HNVMQMGGDFANNPNAQQFIDKMVNKHGFDRQQLQEILSQAKRLDSVLRLMDNQAPTT
                     SVKPPSGPNGAWLRYRKKFITPDNVQNGVVFWNQYEDALNRAWQVYGVPPEIIVGIIG
                     VETRWGRVMGKTRILDALATLSFNYPRRAEYFSGELETFLLMARDEQDDPLNLKGSFA
                     GAMGYGQFMPSSYKQYAVDFSGDGHINLWDPVDAIGSVANYFKAHGWVKGDQVAIMAN
                     GQAPGLPNGFKTKYSISQLAAAGLTPQQPLGNHQQASLLRLDVGTGYQYWYGLPNFYT
                     ITRYNHSTHYAMAVWQLGQAVALARVQ"
     gene            7455..8017
                     /gene="srlA"
                     /locus_tag="OO357_001998"
                     /gene_synonym="gutA"
                     /pseudo
     CDS             7455..8017
                     /gene="srlA"
                     /locus_tag="OO357_001998"
                     /gene_synonym="gutA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026180.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
     gene            8014..8973
                     /gene="srlE"
                     /locus_tag="OO357_001999"
                     /gene_synonym="gutE"
     CDS             8014..8973
                     /gene="srlE"
                     /locus_tag="OO357_001999"
                     /gene_synonym="gutE"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HCR5886955.1"
                     /translation="MTRIRIEKGTGGWGGPLELEATPGKKIVYITAGTRPAIVDKLAQ
                     LTGWQAIDGFKEGEPAEAEIGVAVIDCGGTLRCGIYPKRRIPTINIHSTGKSGPLAQY
                     IVEDIYVSGVKEENITVVGDATPQPSSVGRDYDTSKKITEQSDGLLAKVGMGMGSAVA
                     VLFQSGRDTIDTVLKTILPFMAFVSALIGIIMASGLGDWIAHGLAPLASHPLGLVMLA
                     LICSFPLLSPFLGPGAVIAQVIGVLIGVQIGLGNIPPHLALPALFAINAQAACDFIPV
                     GLSLAEARQDTVRVGVPSVLVSRFLTGAPTVLIAWFVSGFIYQ"
     gene            8984..9355
                     /gene="srlB"
                     /locus_tag="OO357_002000"
                     /gene_synonym="gutB"
     CDS             8984..9355
                     /gene="srlB"
                     /locus_tag="OO357_002000"
                     /gene_synonym="gutB"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HCR5886956.1"
                     /translation="MTVIYQTTITRIGASATDALSDQMLITFREGAPADLEEYCFIHC
                     HGELKGALHPGLQFSLGQHRYPVTAVGSVAEDNLRELGHVTLRFDGLNEAEFPGTVHV
                     AGPVPDDIAPGSVLKFESVKE"
     gene            9359..10138
                     /gene="srlD"
                     /locus_tag="OO357_002001"
     CDS             9359..10138
                     /gene="srlD"
                     /locus_tag="OO357_002001"
                     /EC_number="1.1.1.140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sorbitol-6-phosphate dehydrogenase"
                     /protein_id="HCR5886957.1"
                     /translation="MNQVAVVIGGGQTLGAFLCHGLAAEGYRVAVVDIQSDKAANVAQ
                     EINAEYGEGMAYGFGADATSEQSVLALSRGVDEIFGRVDLLVYSAGIAKAAFISDFQL
                     GDFDRSLQVNLVGYFLCAREFSRLMIRDGIQGRIIQINSKSGKVGSKHNSGYSAAKFG
                     GVGLTQSLALDLAEYGITVHSLMLGNLLKSRMFQSLLPQYATKLGIKPDQVEQYYIDK
                     VPLKRGCDYQDVLNMLLFYASPKASYCTGQSINVTGGQVMF"
     gene            10243..10602
                     /gene="gutM"
                     /locus_tag="OO357_002002"
     CDS             10243..10602
                     /gene="gutM"
                     /locus_tag="OO357_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GutM"
                     /protein_id="HCR5886958.1"
                     /translation="MVSALITVAVIAWCAQLALGGWQISRFNRAFDTLCQQGRVGVDR
                     SSGRFKPRVVVAIALDDQQCIVDTLFMKGLTVFARPQKIPAITGMHAGDLQPDVIFPH
                     DPLSQNALSLALKLKRG"
     gene            10669..11442
                     /gene="srlR"
                     /locus_tag="OO357_002003"
                     /gene_synonym="gutR"
     CDS             10669..11442
                     /gene="srlR"
                     /locus_tag="OO357_002003"
                     /gene_synonym="gutR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucitol operon DNA-binding transcriptional
                     repressor SrlR"
                     /protein_id="HCR5886959.1"
                     /translation="MKPRQRQAAILEYLQKQGKCSVEELAQYFDTTGTTIRKDLVILE
                     HAGTVIRTYGGVVLNKEESDPPIDHKTLINTHKKELIAEAAVSFIHDGDSIILDAGST
                     VLQIVPLLSRFNNITVMTNSLHIVNALSELDNEQTILMPGGTFRKKSASFHGQLAENA
                     FEHFTFDKLFMGTDGIDLNAGVTTFNEVYTVSKAMCNAAREVILMADSSKFGRKSPNV
                     VCSLESVDKLITDAGIDPAFRQALEEKGIDVIITGESNE"
     gene            11435..12400
                     /gene="gutQ"
                     /locus_tag="OO357_002004"
     CDS             11435..12400
                     /gene="gutQ"
                     /locus_tag="OO357_002004"
                     /EC_number="5.3.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose-5-phosphate isomerase GutQ"
                     /protein_id="HCR5886960.1"
                     /translation="MSEALLNTGRQTLMLELQEASRLPERLGDDFVRAANIILHCEGK
                     VVVSGIGKSGHIGKKIAATLASTGTPAFFVHPAEALHGDLGMIESRDVMLFISYSGGA
                     KELDLIIPRLEDKSIALLAMTGKPTSPLGLAAKAVLDISVEREACPMHLAPTSSTVNT
                     LMMGDALAMAVMQARGFNEEDFARSHPAGALGARLLNKVHHLMRRDDAIPQVALTASV
                     MDAMLELSRTGLGLVAVCDAQQQVQGVFTDGDLRRWLVGGGALTTPVNEAMTTGGTTL
                     QAQSRAIDAKEVLMKRKITAAPVVDENGKLTGAINLQDFYQAGII"
     gene            complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO357_002005"
     CDS             complement(12397..13911)
                     /gene="norR"
                     /locus_tag="OO357_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide reductase transcriptional regulator
                     NorR"
                     /protein_id="HCR5886961.1"
                     /translation="MSFSVDVLANIAIELQRGIGHQDRFQRLITTLRQVLECDASALL
                     RYDSRQFIPLAIDGLAKDVLGRRFALEGHPRLEAIARAGDVVRFPADSELPDPYDGLI
                     PGQESLKVHACVGLPLFVGQNLIGALTLDGMQPDQFDVFSDEELRLIAALAAGALSNA
                     LLIEQLESQNMLPGDATPFEAVKQTQMIGLSPGMTQLKKEIEIVAASDLNVLISGETG
                     TGKELVAKAIHEASPRAVNPLVYLNCAALPESVAESELFGHVKGAFTGAISNRSGKFE
                     MADNGTLFLDEIGELSLALQAKLLRVLQYGDIQRVGDDRSLRVDVRVLAATNRDLREE
                     VLAGRFRADLFHRLSVFPLSVPPLRERGDDVILLAGYFCEQCRLRQGLSRVVLSAGAR
                     NLLQHYSFPGNVRELEHAIHRAVVLARATRNGDEVILEAQHFAFPEVTLPPPEAAAVP
                     VVKQNLREATEAFQRETIRQALAQNHHNWAACARMLETDVANLHRLAKRLGLKD"
     gene            14098..15537
                     /gene="norV"
                     /locus_tag="OO357_002006"
     CDS             14098..15537
                     /gene="norV"
                     /locus_tag="OO357_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic nitric oxide reductase
                     flavorubredoxin"
                     /protein_id="HCR5886962.1"
                     /translation="MSIVVKNNIHWVGQRDWEVRDFHGTEYKTLRGSSYNSYLIREEK
                     NVLIDTVDHKFSREFVQNLRNEIDLADIDYIVINHAEEDHAGALTELMAQIPDTPIYC
                     TANAIDSINGHHHHPEWNFNVVKTGDTLDIGNGKQLIFVETPMLHWPDSMMTYLTGDA
                     VLFSNDAFGQHYCDEHLFNDEVDQTELFEQCQRYYANILTPFSRLVTPKITEILGFNL
                     PVDMIATSHGVVWRDNPTQIVELYLKWAADYQEDRITIFYDTMSNNTRMMADAIAQGI
                     AETDPRVAVKIFNVARSDKNEILTNVFRSKGVLVGTSTMNNVMMPKIAGLVEEMTGLR
                     FRNKRASAFGSHGWSGGAMDRLSTRLQDAGFEMSLSLKAKWRPDQDALELCREHGREI
                     ARQWALAPLPQSTVNTVVKEETSATTTADLGPRMQCSVCQWIYDPAKGEPMQDVAPGT
                     PWSEVPDNFLCPECSLGKDVFDELASEAK"
     gene            15534..16667
                     /gene="norW"
                     /locus_tag="OO357_002007"
     CDS             15534..16667
                     /gene="norW"
                     /locus_tag="OO357_002007"
                     /EC_number="1.18.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavorubredoxin reductase NorW"
                     /protein_id="HCR5886963.1"
                     /translation="MSNGIVIIGSGFAARQLVKNIRKQDATIPLTLIAADSIDEYNKP
                     DLSHVISQGQRADDLTRQTAGEFAGQFNLRLFPHTWVTDIDAEAHVVKSQNNQWQYDK
                     LVLATGASAFVPPVPGRELMLTLNSQQEYRACETQLRDARRVLIVGGGLIGSELAMDF
                     CRAGKAVTLIDNAASILASLMPPEVSSRLQHRLTEMGVHLLLKSQLQGLEKTDSGILA
                     TLDHQRSIEVDAVIAATGLRPETALARRAGLTINRGVCVDSYLQTSNADIYALGDCAE
                     INGQVLPFLQPIQLSGMVLAKNLLGNNTPLKLPAMLVKIKTPELPLHLAGETQRQDLR
                     WQINTERQGMVARGVDDADQLRAFVVSEDRMKEAFVLLKTLPV"
     gene            complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO357_002008"
     CDS             complement(16795..19047)
                     /gene="hypF"
                     /locus_tag="OO357_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyltransferase HypF"
                     /protein_id="HCR5886964.1"
                     /translation="MAKNTSCGVQLRIRGKVQGVGFRPFVWQLAQQLNLHGDVCNDGD
                     GVEVRLLEDPETFLVQLHQHCPPLARIDSVEREPFIWSQLPTEFTIRQSTGGTMNTQI
                     VPDAATCPACLAEMNTPGERRYRYPFINCTHCGPRFTIIRAMPYDRPFTVMAAFPLCP
                     ACDKEYRDPLDRRFHAQPVACPECGPHLEWVSHGEHAEQEAALQAAIAQLKMGNIVAI
                     KGIGGFHLACDARNSNAVATLRERKHRPAKPLAVMLPVAESLPDAARQLLTTPAAPIV
                     LVDKKYVPELCDDIAPGLNEVGVMLPANPLQHLLLQELQCPLVMTSGNLSGKPPAISN
                     EQALADLQGIADGFLLHNRDIVQRMDDSVVRESGEMLRRSRGYVPDALVLPPGFKNVP
                     PVLCLGADLKNTFCLVRGEQAVLSQHLGDLSDDGIQMQWREALRLMQNIYDFTPQYIV
                     HDVHPGYVSSQWASEMNLPTQTVLHHHAHAAACLAEHQWPLDGGDVIALTLDGIGMGE
                     NGALWGGECLRVNYRECEHLGGLPAVALPGGDLAAKQPWRNLLAQCLRFVPEWQNYPE
                     TASVQQQNWSVLARAIERGINAPLASSCGRLFDAVAAALGCAPATLSYEGEAACALEA
                     LAASCDGVTHPVTMPRVDNQLDLATFWQQWLNWQALVNQRAWAFHDALAQGFAALMRE
                     QATMRGITTLVFSGGVIHNRLLRARLAHYLADFTLLFPQSLPAGDGGLSLGQGVIAAA
                     RWLAGEVQNG"
     gene            complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO357_002009"
     CDS             complement(19200..19727)
                     /gene="hydN"
                     /locus_tag="OO357_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708520.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport protein HydN"
                     /protein_id="HCR5886965.1"
                     /translation="MNRFIIADASKCIGCRTCEVACVVSHQENQDCASLTPETFLPRI
                     HVIKGVNISTATVCRQCEDAPCANVCPNGAISRDKGFVPVMQERCIGCKTCVVACPYG
                     AMEVVVRPVIRNSGAGLNVRADKAEANKCDLCNHREDGPACMAACPTHALICVDRNKL
                     EQLSAEKRRRTALMF"
     gene            complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO357_002010"
     CDS             complement(19876..20886)
                     /gene="ascG"
                     /locus_tag="OO357_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator AscG"
                     /protein_id="HCR5886966.1"
                     /translation="MTTMLEVAKRAGVSKATVSRVLSGNGYVSQETKDRVFQAVEESS
                     YRPNLLARNLSAKSTQTLGLVVTNTLYHGIYFSELLFHAARMAEEKGRQLLLADGKHS
                     AEEERQAIQYLLDLRCDAIMIYPRFLSVDEIDDIIDAHSQPIMVLNRRLRNNSSHSVW
                     CDHKQTSFNAVAELINAGHQEIAFLTGSMDSPTSIERLAGYKDALSQHGIALNEKLIA
                     NGKWTPASGAEGVETLLERGAKFSALVASNDDMAIGAIKALHERGVAVPEQVSVIGFD
                     DIAIAPYIVPALSSVKIPVTEMIQEIIGRLIFMLDGGDFSPPKTFSGKLIRRDSLIAL
                     SR"
     gene            complement(20900..21019)
                     /locus_tag="OO357_002011"
     CDS             complement(20900..21019)
                     /locus_tag="OO357_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur protein"
                     /protein_id="HCR5886967.1"
                     /translation="MFTFIKKVIKTGTATSSYPLEPIAQGQSANGAVHCVDCV"
     gene            complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO357_002012"
     CDS             complement(21029..22738)
                     /gene="hycE"
                     /locus_tag="OO357_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycE"
                     /protein_id="HCR5886968.1"
                     /translation="MSEEKLGQHYLAALNEAFLGVVLDHAWQTKDQLTVTVKVNYLPE
                     VVEFLYYKQGGWLSVLFGNDERKLNGHYAVYYVLSMEKGTKCWVTVRVEVDANKPEYP
                     SVTPRVPAAVWGEREVRDMYGLIPVGLPDERRLVLPDDWPDELYPLRKDSMDYRQRPA
                     PTTDAETYEFINELGDKKNNVVPIGPLHVTSDEPGHFRLFVDGENIIDADYRLFYVHR
                     GMEKLAETRMGYNEVTFLSDRVCGICGFAHSTAYTTSVENAMGIQVPERAQMIRAILL
                     EVERLHPHLLNLGLACHFTGFDSGFMQFFRVRETSMKMAEILTGARKTYGLNLIGGIR
                     RDLLKDDMIQTRQLAQQMRREVQELVDVLLSTPNMEQRTVGIGRLDPEIARDFSNVGP
                     MVRASGLARDTRADHPFVGYGLLPMEVHSEQGCDVISRLKVRINEVYTALNMIDYGLD
                     NLPGGPLMVEGFTYIPHRFALGFAEAPRGDDIHWSMTGDNQKLYRWRCRAATYANWPT
                     LRYMLRGNTVSYAPLIIGSLDPCYSCTDRMTVVDVRKKKSKVVPYKELERYSIERKNS
                     PLK"
     gene            complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO357_002013"
     CDS             complement(22756..23679)
                     /gene="hycD"
                     /locus_tag="OO357_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycD"
                     /protein_id="HCR5886969.1"
                     /translation="MSVLYPLIQALVLFAVAPLLSGITRVARARLHNRRGPGVLQEYR
                     DIIKLLGRQSVGPDASGWMFRLTPYVMVGVMLTIATALPVVTVGSPLPQLGDLITLLY
                     LFAIARFFFAISGLDTGSPFTAIGASREAMLGVLVEPMLLLGLWVAAQVAGSTNISNI
                     TDTVYHWPLSQSIPLVLALCACAFATFIEMGKLPFDLAEAEQELQEGPLSEYSGSGFG
                     VMKWGISLKQLVVLQMFVGVFIPWGQMETFTVGGLLLALVIAIVKLVVGVLVIALFEN
                     SMARLRLDITPRITWAGFGFAFLAFVSLLAA"
     gene            complement(23682..25493)
                     /gene="hycC"
                     /locus_tag="OO357_002014"
     CDS             complement(23682..25493)
                     /gene="hycC"
                     /locus_tag="OO357_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005092744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit 3"
                     /protein_id="HCR5886970.1"
                     /translation="MSPPLFWHFSFQKALSGWIAGIGGAVGSLYTAAAGFTVLTGAVG
                     VSGALSLVSYDVQISPLNAIWLITLGLCGLFVSLYNIDWHRHAQVKCNGLQINMLMAA
                     AVCAVIASNLGMFVVMAEVMALCAVFLTSNSKEGKLWFALRRLGTLLLVIACWLLWQR
                     YGTLDLRLLDMRMQQLPLGSDIWLLGVIGFGLLAGIIPLHGWVPQAHANASAPAAALF
                     STVVMKIGLLGMLTLSLLGGNAPLWWGIALLVLGMITAFVGGLYALMEHNIQRLLAYH
                     TLENIGIILLGLGLGLGLGLGLGAGVTGIALEQPALIALGLVGGLYHLLNHSLFKSVL
                     FLGAGSVWFRTGHRDIEKLGGIGKKMPVISIAMLVGLMAMAALPPLNGFAGEWVIYQS
                     FFKLSNSGAFVARLLGPLLAVGLAITGALAVMCMAKVYGVTFLGAPRTKEAENATCAP
                     LLMSVSVVALAICCVIGGVAAPWLLPMLSAAVPLPLEPANTTVSQPMITLLLIACPLL
                     PFIIMAICKGDRLPSRSRGAAWVCGYDHEKSMVITAHGFAMPVKQAFAPVLKLRKWLN
                     PVSLVPGWQCEGSALLFRRMALVELAVLVVIIVSRGA"
     gene            complement(25530..26141)
                     /gene="hycB"
                     /locus_tag="OO357_002015"
     CDS             complement(25530..26141)
                     /gene="hycB"
                     /locus_tag="OO357_002015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase subunit HycB"
                     /protein_id="HCR5886971.1"
                     /translation="MNRFVIADSTLCIGCHTCEAACSETHRQHGLQSMPRLRVMLNEK
                     ESAPQLCHHCEDAPCAVVCPVNAITRVDGAVQLNESLCVSCKLCGIACPFGAIEFSGS
                     RPLDIPANANTPKAPPAPPAPARVSTLLDWVPGIRAIAVKCDLCSFDEQGPACVRMCP
                     TKALHLVDNTDIARVSKRKRELTFNTDFGDLTLFQQAQSGEAK"
     gene            complement(26266..26727)
                     /gene="hycA"
                     /locus_tag="OO357_002016"
     CDS             complement(26266..26727)
                     /gene="hycA"
                     /locus_tag="OO357_002016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase regulator HycA"
                     /protein_id="HCR5886972.1"
                     /translation="MTIWEISEKADYIAQRHRRLQDQWHIYCNSLVQGITLSKARLHH
                     AMSCAPDKELCFVLFEHFRIYVTLADGFNSHTIEYYVETKDGEDKQRIAQAQLSIDGM
                     IDGKVNIRDREQVLEHYLEKIAGVYDSLYTAIENNVPVNLSQLVKGQSPAA"
     gene            26939..27289
                     /gene="hypA"
                     /locus_tag="OO357_002017"
     CDS             26939..27289
                     /gene="hypA"
                     /locus_tag="OO357_002017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation nickel metallochaperone
                     HypA"
                     /protein_id="HCR5886973.1"
                     /translation="MHEITLCQRALELIEQQAAKHGAKRVTGVWLKIGAFSCVETSSL
                     AFCFDLVCRGSVAEGCKLHLEEQEAECWCETCQQYVTLLTQRVRRCPQCHGDMLQIVA
                     DDGLQIRRIEIDQE"
     gene            27293..28165
                     /gene="hypB"
                     /locus_tag="OO357_002018"
     CDS             27293..28165
                     /gene="hypB"
                     /locus_tag="OO357_002018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase nickel incorporation protein HypB"
                     /protein_id="HCR5886974.1"
                     /translation="MCTTCGCGEGNLYIEGDEHNPHSAFRSAPFAPAARPQMKITGIK
                     APEFTPSQTEEGDLHYGHGEAGTHTPGMSQRRMLEVEIDVLDKNNRLAERNRARFAAR
                     KQLVLNLVSSPGSGKTTLLTETLMRLKDSVPCAVIEGDQQTVNDAARIRATGTPAIQV
                     NTGKGCHLDAQMIADAAPRLPLDDNGILFIENVGNLVCPASFDLGEKHKVAVLSVTEG
                     EDKPLKYPHMFAAASLMLLNKVDLLPYLNFDVEKCIACAREVNPEIEIILISATSGEG
                     MDQWLNWLETQRCA"
     gene            28156..28428
                     /gene="hypC"
                     /locus_tag="OO357_002019"
     CDS             28156..28428
                     /gene="hypC"
                     /locus_tag="OO357_002019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation protein HypC"
                     /protein_id="HCR5886975.1"
                     /translation="MCIGVPGQIRTIDGNQAKVDVCGIQRDVDLTLVGSCDENGQPRV
                     GQWVLVHVGFAMSVINEAEARDTLDALQNMFDVEPDVGALLYGEEK"
     gene            28428..29549
                     /gene="hypD"
                     /locus_tag="OO357_002020"
     CDS             28428..29549
                     /gene="hypD"
                     /locus_tag="OO357_002020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HCR5886976.1"
                     /translation="MRFVDEYRAPEQVMQLIEHLRERASHLSYTAERPLRIMEVCGGH
                     THAIFKFGLDQLLPENVEFIHGPGCPVCVLPMGRIDTCVEIASHPEVIFCTFGDAMRV
                     PGKQGSLLQAKARGADVRIVYSPMDALKLAQENPTRKVVFFGLGFETTMPTTAITLQQ
                     AKARDVQNFYFFCQHITLIPTLRSLLEQPDNGIDAFLAPGHVSMVIGTDAYNFIASDF
                     HRPLVVAGFEPLDLLQGVVMLVEQKIAAHSKVENQYRRVVPDAGNLLAQQAIADVFCV
                     NGDSEWRGLGVIESSGVHLTPDYQRFDAEAHFRPAPQQVCDDPRARCGEVLTGKCKPH
                     QCPLFGNTCNPQTAFGALMVSSEGACAAWYQYRQQESEA"
     gene            29546..30556
                     /gene="hypE"
                     /locus_tag="OO357_002021"
     CDS             29546..30556
                     /gene="hypE"
                     /locus_tag="OO357_002021"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase maturation carbamoyl dehydratase
                     HypE"
                     /protein_id="HCR5886977.1"
                     /translation="MNNIQLAHGSGSQAMQQLINSLFMEAFANPWLAEQEDQARLDLA
                     QLVAEGDRLAFSTDSYVIDPLFFPGGNIGKLAICGTANDVAGSGAIPRYLSCGFILEE
                     GLPMETLKAVVTSMAETARTAGIAIVTGDTKVVQRGAADKLFINTAGMGAIPANIHWG
                     AQTLTAGDVLLVSGTLGDHGATILNLREQLGLDGELVSDCAVLTPLIQTLRDIPGVKA
                     LRDATRGGVNAVVHEFAAACGCGIEISESALPVKPAVRGVCELLGLDALNFANEGKLV
                     IAVERNAAEQVLAALHSHPLGKDAALIGEVVERKGVRLAGLYGVKRTLDLPHAEPLPR
                     IC"
     gene            30630..32708
                     /gene="flhA"
                     /locus_tag="OO357_002022"
     CDS             30630..32708
                     /gene="flhA"
                     /locus_tag="OO357_002022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate hydrogenlyase transcriptional activator
                     FlhA"
                     /protein_id="HCR5886978.1"
                     /translation="MSYTPMSDLGQQGLFDITRTLLQQPDLASLCEALSQLVKRSALA
                     DNAAIVLWQAQTQRASYYASREKDTPIKYEDETVLAHGPVRSILSRPDTLHCSYEEFC
                     ETWPQLAAGGLYPKFGHYCLMPLAAGGHIFGGCEFIRYDERPWSEKEFNRLQTFTQIV
                     SVVTEQIQSRVVNNVDYELLCRERDNFRILVAITNAVLSRLDMDELVSEVAKEIHYYF
                     DIDDISIVLRSHRKNKLNIYSTHYLDKQHPAHEQSEVDEAGTLTERVFKSKEMLLINL
                     YERDDLAPYERMLFDTWGNQIQTLCLLPLMSGDTMLGVLKLAQCEEKVFTTTNLNLLR
                     QIAERVAIAVDNALAYQEIHRLKERLVDENLALTEQLNNVDSEFGEIIGRSEAMYSVL
                     KQVEMVAQSDSTVLILGETGSGKELIARAIHNLSGRNNRRMVKMNCAAMPAGLLESDL
                     FGHERGAFTGANAQRIGRFELADKSSLFLDEVGDMPLELQPKLLRVLQEQEFERLGSN
                     KIIQTDVRLIAATNRDLKKMVADREFRSDLYYRLNVFPIHLPPLRERPEDIPLLAKAF
                     TFKIARRLGRNIDSIPAETLRTLSNMEWPGNVRELENVIERAVLLTRGNVLQLSLPDI
                     ALPEPETPPAATVVAQEGEDEYQLIVRVLKETNSVVAGPKGAAQRLGLKRTTLLSRMK
                     RLGIDKSALI"
     gene            complement(32745..33098)
                     /locus_tag="OO357_002023"
     CDS             complement(32745..33098)
                     /locus_tag="OO357_002023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrous oxide-stimulated promoter family
                     protein"
                     /protein_id="HCR5886979.1"
                     /translation="MSGKRISREKLTIKKMIDLYQAKCPQASAEPEHYEALFVYAQKR
                     LDKCVFGEEKPACKQCPVHCYQPAKREEMKQIMRWAGPRMLWRHPILTVRHLIDDKRP
                     VPELPEKYRPKKPRE"
BASE COUNT         7809 a         9237 c         8625 g         7470 t
ORIGIN      
        1 atggcggtga ggcggggatt cgaaccccgg atgcagcttt tgaccgcata ctcccttagc
       61 aggggagcgc cttcagcctc tcggccacct caccacacgc ctcttacgag tgcttcgaag
      121 aacttgttta tcgctcatca tcgctgcgtg gcgcacatat tactttctga gacttataag
      181 tcaaacaatt tttcccacac ttttatcgtt tgcacacttc acgctcaatt agtctgtaaa
      241 agcgtcaaaa agagtgtttt atctacagaa aaacggaggt ctggcagata gcagtaatgc
      301 aaaaaaatgg agacttaagt tgaatgaacg ggagtaaagc gaaaagacta tagagtgaag
      361 gagaaatttt gagggtgcgt ctcaccgata aagatgagac gcggaaagat tagtaactgg
      421 actgctggga tttttcagcc tggatacgct ggtagatctc ttcacggtga acagaaactt
      481 ccttcggggc atttacgcca atacgtacct ggttgccctt tacccctaaa actgtcacgg
      541 tgacctcatc cccaatcatg agggtctcac caactcgacg agtcagaatc agcattcttt
      601 gctccttgaa agattaaaag agtcgggtct ctctgtatcc cggcattatc catcatataa
      661 cgccaaaaag taagcaatga caaacacatt acatgtaagc agtcatggca ttacattctg
      721 ttaaacctaa gtttagccga tatacacaac ttcaacctga ctttatcgtt gtcgatagcg
      781 ttaatgcgaa tgccgtgaag cgagtccacg gcattgcctg acgcttatat tattgcaatt
      841 tcgcgctgac ccagcctttc acactggcta acgctgcagg taaggccgca gcatccgtac
      901 caccggcttg cgccatgtca ggacgtccac cacccttgcc gcccacctgc tgagcgacca
      961 taccaatcag ttcccctgct ttcacacgat ctgtgacgtc cttagatacg cctgcaatca
     1021 gagaaacctt accttcggct accgttgcca gcacgataat tgtcgacccc agctgatttt
     1081 ttaaatcgtc aaccatggta cgcaacattt tcggctcaac accgctaagc tcgctaacca
     1141 acagcttaac accattaaca tcaattgcct tactggaaag atttgcgctc tcctgtgcgg
     1201 cagcttgttc tttaagctgt tgtagttctt tttctaactg acgcgtacgt tccagtactg
     1261 agcgcacttt atccgccaga ttattgctat cgcctttcag cagatgcgcg acttcgctta
     1321 agcgatcgct gtctgcatga acggtggtga tagcgccttc tccggttacc gcttcgatac
     1381 gacgaacgcc tgcagcagta cccgattcag agatgatgcg gaacagacca atatcaccag
     1441 tgcggctggc gtgagtaccg ccacacagct cggtggagaa atcgcccatg ctcagcacgc
     1501 gtacacgctc atcatacttc tcgccgaaca gcgccatcgc acctttcgct ttcgccgctt
     1561 cgagattcat gatgttggtt tcgatcggca aattgcgacg aatctgtgcg ttcaccaggt
     1621 cttcgaccgc acgaatctct tccggtttca tcgcttcgtt gtgtgagaag tcgaagcgca
     1681 gcaccttgtc gttaaccagt gaacctttct gcgatacatg agtccccaga acctggcgca
     1741 gcgcagcgtg catcaggtgc gttgcggagt gattcagacg aatacgggcg cgacgagcct
     1801 catcaatatc agcctgcaca gcgtcgccca ctttcagaga acccgcagca agtttaccga
     1861 tgtgaccaat cgcctggccg tatttctgcg tatcttccac cgcgaaggag aagttagcgc
     1921 ctttcagttc gcctttatca ccaacctgac cgccggattc cgcatagaat ggcgtttgat
     1981 ccagcacgac cacagcttcc tggccggcat tgatggcatc aaccgcttta ccatcaacaa
     2041 acagcgcggt cactttgccg ttcagttcca gatggtcata gcctttaaat tcagatgcac
     2101 tatcaacacg gatcattgcg ttgtaatcgg caccaaagcc gctggcttcg cgcgcgcgac
     2161 gacgctgctc ttccattgct gcttcaaaac cagcttcgtc aactttgatg ttgcgctcac
     2221 gacaaacatc agccgtcagg tcaaccggga agccataggt gtcgtacaga cggaaagccg
     2281 tttcaccatc cagcgtatca ccagaaagtt ttgccagctc ttcatccagc aacgccagac
     2341 cgcgctccag agtacgggca aactgctctt cttcagtctt cagcacctgc tcaacctgcg
     2401 cctgctggcg tttcaggtct tcacccgcag agcccataac gtcgatcagc ggaccaacca
     2461 gtttgtagaa gaaggtttct ttcgcgccga gcatattgcc gtgacgcact gcgcgacgaa
     2521 tgatacgacg cagtacataa ccacggtttt cattggacgg cattacgcca tccgcgatca
     2581 ggaacgcaca agaacgaatg tggtcagcga ttacgcgcag cgatttattg ctcagatcgg
     2641 tcgcgccagt gactttcgct accgcctgga tcaacgtgcg gaacaggtcg atgtcatagt
     2701 tagagttaac gtgttgcagc accgcagcaa tacgctccag acccataccg gtatctacag
     2761 acggcttcgg cagcggttcc atcgtgccat ctgcctggcg gttgaactgc atgaagacga
     2821 tgttccagat ctcaatgtag cggtcgccgt cttcttccgg acttcccgga gggccacccc
     2881 aaatgtggtc gccgtgatcg tagaagattt cggtgcacgg gccgcacgga ccagtgtcac
     2941 ccatctgcca gaagttgtca gatgcgtatg gcgcaccttt gttatcgccg atgcgaataa
     3001 tacgttcgcg cgggatcccg acttcttttt cccagatttc gtaggcttcg tcgtcgcttt
     3061 catagacggt aacccacaga cgctctttcg gcagggcaaa ccatttttcg ctggtcagca
     3121 gttcccatgc aaactgaatg gcatcgtgtt tgaaatagtc gccgaagctg aagttgccca
     3181 gcatttcgaa gaaggtatgg tgacgcgcgg tgtaaccgac gttttccagg tcgttgtgtt
     3241 taccacccgc acgcacgcag cgttgggaag tggtagcgcg ggaataatta cgcttgtcga
     3301 gcccaaggaa cacatccttg aactggttca tcccggcgtt ggtaaacaac aaagttgggt
     3361 cgttatgggg taccagggag ctgctggcaa ctacctgatg tcccttacta tggaaaaagt
     3421 cgagaaacgc ctgacggatc tcagcggtgc tcttgctcat aattatcctg aaatcaagct
     3481 aacgaaatat cgccaccagc tccagcgtgt cttaaccgcc gggctggtaa ctgaaaagtg
     3541 ggaataagat aagttttctt gactgggaag taaaataccg tatgcgttca gtcggcaaaa
     3601 tttcgccaaa tatcctggat atcttccatc agatagccac gatagagcag aaaacgctgg
     3661 atcttaactt tttctgaaaa gacagttggc aaaggttcac catattttcg cgtcgcctga
     3721 tcgcgcgcca gtgcgcacca gtcgatgtca cattcacgca tcgctttttc tgtcgcttcg
     3781 cgggaaatac ctttctgatt cagttcctga cgaatacgtg caggtccata ccctttgcgg
     3841 ctacggctgg cgataaagcg cgcaacaaat cggctgtcat cgagatagcc atgttcatgg
     3901 caccaggcaa taacgcgctc gtaatcttct gccgtagcat caatctcttc tgggccattt
     3961 ttgcccataa tcggtgccgc gagtttacgt cgcagttctt gctcactgtg atcgcgcacc
     4021 gccagaatgc gtaccgcacg atccaacagg cgagcatatg ccgggcgacg ggatgttgat
     4081 tctgtcatgg catatcctta caacttaaaa aagcaaaagg gccgcagatg cgccccaggt
     4141 tttgctgata aacagattta atagtcaatg agtatgcaga atgttcccgc aggcccaatt
     4201 cgttagagga tcgaggtgag agtaattttt cacgccagtg ctggcctgtt cccctcaccc
     4261 taaccctctc cccaaagggg cgaggggacc gtccgtgcac acattatcta ttatcatcaa
     4321 taacgaacga ttaaaaatct tcgttagttt ctgctactcc ttcgctgtca tctacagaga
     4381 aatccggcgt tgagttcggg ttgctcagca gcaactcacg tactttcttc tcgatctctt
     4441 tcgcggtttc cgggttatct ttcagccagg cagttgcatt cgctttaccc tgaccgatct
     4501 tctcaccttt gtagctgtac cacgcgcctg ctttctcgat cagcttctct tttacgccca
     4561 ggtcaaccag ttcgccgtag aagttgatac cttcgccgta gaggatctga aattcagcct
     4621 gtttaaacgg tgcagcgatt ttgttcttca ccactttcac gcgggtttcg ctacccacca
     4681 cgttttcgcc ctctttcacc gcgccgatac gacggatgtc gagacgaaca gaggcgtaga
     4741 atttcagcgc gttaccaccg gtagtggttt ccgggttacc gaacatcaca ccaattttca
     4801 tacggatctg gttgatgaag atcagcagcg tgttggactg cttcaggtta cccgccagct
     4861 tacgcatcgc ctggctcatc atacgtgccg caaggcccat gtgagagtcg ccgatttcgc
     4921 cttcgatttc cgctttcggc gtcagtgccg ccacggagtc aacgacgata acgtctactg
     4981 cgccagaacg cgccagggcg tcacagattt ccagtgcctg ctcgccggta tccggctggg
     5041 agcacagcag gttatcgata tcgacgccca gtttacgtgc gtagattggg tccagcgcgt
     5101 gttcagcatc gataaacgca caggttttac cttcacgctg cgctgcggcg atcacctgca
     5161 gcgtcagcgt ggttttaccg gaagattccg gtccgtagat ttcgacgata cggcccatcg
     5221 gcagaccacc tgccccaagc gcgatatcca gtgaaagcga accggtagag atggtttcca
     5281 catccatgga acggtcttca cccaggcgca tgatggagcc tttaccaaat tgtttctcaa
     5341 tctggcccag tgctgccgcc aacgctttct gtttgttttc gtcgatagcc atttttactc
     5401 ctgtcatgcc gggtaatacc ggatagtcaa tatgttctgt tgaaacaatt atactgtatg
     5461 ctcatacagt atcaagtgtt ttgtagaaat tgttgccaca aggtctgcaa tgcatacgca
     5521 gtagcctgac gacgcaccgc atcacggtcg ccgctgaagc attcccgccg ggtaatgcct
     5581 tcaccgcggg cagtggcaaa agcaaaccag acggtgccga caggcttctc ttcactgccg
     5641 ccatccggcc cggcgatacc actaatagac acggcataat cagcacgagc cgctttcagt
     5701 gcacctatcg ccatttccac cacgacgggt tcactcaccg cgccatgctg cgccagcgtc
     5761 tcttcgcgta cgccgatcat ctgcgctttg gcttcgttac tgtaggtgac aaatccgcgt
     5821 tcaaaccagg cggagctacc ggcaatatcg gtaatcactt tcgctaccca accaccggta
     5881 caagactcgg cagttgttac ggttgcgcca cgggctttca gcgcctgccc aacctgttcg
     5941 cttaactgca tcagttcact gtcagtcatc acaacctctg tcagtcaaaa atttatgccg
     6001 gacaagatag cactttcgtc aggaagatgg tgttggcgtt gcaattttac gaggaggatt
     6061 cagaaaaaag ctgattagcc agagggaagc tcacgccccc ctcttgtaaa tagctactgt
     6121 actcgcgcca gcgccacggc ttgtcctaac tgccagaccg ccattgcgta atgggtgctg
     6181 tggttgtaac gggtgatggt gtagaagttc ggcagaccgt accagtactg gtagccggtg
     6241 ccaacatcca gacgcagcag gctggcttgt tgatggttgc ccagcggctg ctgtggcgtt
     6301 aaacctgcgg cggcaagctg cgaaatgctg tacttagttt tgaaaccatt tggcaagcct
     6361 ggagcctgac cgtttgccat tatcgcgacc tgatcgcctt tcacccagcc gtgcgctttg
     6421 aaatagttcg ccacgctacc gatcgcatca accggatccc acaggttgat atgcccgtcg
     6481 ccgctgaaat ctaccgcata ttgtttgtaa gacgacggca taaactgtcc gtagcccatc
     6541 gccccggcaa aggaaccttt cagattgagc ggatcgtcct gctcgtcgcg cgccatcagc
     6601 aggaaggttt ccagctcgcc agagaaatac tccgcgcggc gtgggtagtt aaatgacagc
     6661 gttgccagcg catcgaggat gcgagttttc cccatcacgc gcccccagcg ggtttcaacg
     6721 ccgataatcc cgacgataat ttccggcggt acgccataca cctgccacgc gcgattcaac
     6781 gcatcttcat actgattcca gaaaaccaca ccgttctgca cgttgtccgg cgtaataaat
     6841 tttttgcgat aacgcagcca tgcgccgttc ggacctgatg gcggtttcac cgatgtggtt
     6901 ggtgcctggt tatccatcag ccgcagtacc gaatccagac gcttcgcctg ggagagaatt
     6961 tcctgcaact gctgacgatc gaaaccgtgt ttgttcacca ttttgtcgat gaactgctgg
     7021 gcattcgggt tattagcgaa atcgccgccc atctgcatca cattgtgctg cggctcaagc
     7081 aggaagccgc cagacggcgt tccggtggtc gtttcagtct cagtaggttt tggcttgctg
     7141 ctacaggcgg caagcaacac aaaaagggga agcaatgtta cataacgacg cttgaacatg
     7201 aggggtccat ttaacagatt caaccagggg caagtatggt aaagcatcac accccgcaca
     7261 aggaagcggt agtcactgcc cgatacggac tttacataac tcaactcatt cccctcgcta
     7321 tacttttatt caaactttca aattaaaata tttatctttc attttacgat caaaataaca
     7381 cttttaaacc tttcaacctg attagattag gttgccgttt ggtaataaaa caataaatcc
     7441 tgaaggagag aacaatgata gaaaccatta ctcatggtgc agagtggttt atcgggctgt
     7501 tccaaaaggg cggagaggtg tttaccggga tggtgaccgg cattcttccg ctgttgatta
     7561 gcctgcaggt tatcatgaac gcactgatta attttatcgg tcagcatcgt attgaacgtt
     7621 ttgctcaacg ttgcgccggt aaccctgttt cccgttacct actgttaccg tgcattggca
     7681 cgtttgtctt ttgcaatccg atgaccttaa gcctgggtcg ctttatgccg gaaaagtaca
     7741 aacccagcta ctacgcggcg gcctcttata gctgccactc aatgaatggc ctcttccccc
     7801 atatcaaccc tgcgaactgt ttgtttatct tggcattgcc agcggtctga caacgctgaa
     7861 cctgccactt ggcccactgg cggtgagtta tctgctggtt ggtctggtca ccaatttctt
     7921 ccgcggctgg gtgaccgatc tgaccaccgc catttttgag aaaaagatgg gcattcaact
     7981 tgaacaaaaa gttcacctgg caggagcaac atcatgacgc gtattcggat cgaaaaagga
     8041 acgggtggct ggggcggccc gcttgagctg gaagccacgc cgggcaaaaa aatcgtctat
     8101 atcaccgccg gtacccggcc tgcgattgtt gacaaactgg cacaacttac tggctggcag
     8161 gctattgacg gatttaaaga aggtgaaccc gcggaggcgg aaattggtgt cgcggtaatc
     8221 gactgtggcg gcacattacg ctgcggcatc tatccgaaac gacgtattcc caccattaat
     8281 atccactcga cgggcaagtc cggtccgctg gcgcagtaca ttgtggaaga tatttatgtc
     8341 tctggcgtaa aagaagaaaa catcactgta gtgggtgatg cgacaccaca accctcttcc
     8401 gtgggccgtg actatgacac cagtaagaaa atcaccgaac aaagcgatgg tttactggcg
     8461 aaggtgggaa tgggcatggg gtccgccgtt gccgtgttgt ttcagtctgg tcgtgacacc
     8521 atcgacactg tattaaaaac cattctgccg tttatggcat tcgtctcggc gctcattggc
     8581 atcattatgg cttctggcct tggtgactgg attgcccacg gtcttgctcc gctagcgagc
     8641 catccactgg gtctggtcat gctggcgctc atctgctcct tcccactgct ttcacctttc
     8701 ctcggcccag gcgcagttat cgcacaggtt atcggcgtat tgattggcgt gcagattggt
     8761 ctcggcaata ttccgccgca tctggcttta ccggcactgt ttgccatcaa cgcgcaggcg
     8821 gcttgcgact tcatcccggt cggtttgtcg ctggcggaag cccgtcagga cacggttcgc
     8881 gtcggtgtcc cttctgtact ggtgagccgc tttttaaccg gcgcgccgac tgtactgatc
     8941 gcctggtttg tctccggttt tatctatcaa tagaggctga aacatgaccg ttatttatca
     9001 gaccaccatc acccgtatcg gcgcgagtgc cactgacgcc ctcagcgacc agatgctcat
     9061 cacctttcgt gaaggcgcgc cagcggacct cgaagagtat tgcttcattc attgccacgg
     9121 cgagttgaaa ggtgcactcc atcccggttt gcaattttca ctcgggcagc atcgctatcc
     9181 ggtgaccgct gttggcagcg tggcggaaga caaccttcgc gaactgggtc atgtcaccct
     9241 gcgcttcgat ggtttaaacg aagcggaatt tccgggcact gtccatgtgg caggccctgt
     9301 acccgacgat atcgcgccgg gatcggtttt gaagtttgaa tctgttaagg agtaaaaaat
     9361 gaatcaggtt gccgttgtca tcggtggtgg gcaaacctta ggcgcgttcc tgtgccacgg
     9421 tctggctgcc gaggggtatc gcgtcgcggt tgtcgatatt cagagcgaca aagccgcaaa
     9481 tgtggcacaa gaaattaacg ccgaatatgg tgaaggtatg gcgtacggtt ttggtgctga
     9541 cgccactagc gagcaaagcg ttctggcgct ctctcgtggg gtagatgaaa tctttggtcg
     9601 cgtggatttg ctggtctata gcgccggaat agcaaaagca gcctttatca gcgacttcca
     9661 gctcggcgat tttgaccgtt cgctacaagt gaatctggtg ggttatttcc tgtgtgcccg
     9721 tgaattttcg cgtttgatga tccgcgacgg gattcagggt cgcattattc agatcaactc
     9781 gaaatccggc aaagtgggca gcaaacacaa ctctggctac agcgcagcga aatttggtgg
     9841 cgtcgggctg actcaatcac tggcgctgga tctggcggag tacggcatta cggtgcattc
     9901 actgatgctc ggtaacctgc tgaaatcgcg gatgttccag tcactgttgc cacaatacgc
     9961 gaccaagctg ggtatcaaac cggatcaagt cgagcagtat tacatcgaca aagtaccgct
    10021 caaacgcggc tgcgattatc aagatgtgct gaatatgctg ctgttctacg ccagtcctaa
    10081 ggcgtcgtac tgcaccggac agtcgatcaa tgtcaccggc ggtcaggtga tgttctgatc
    10141 aacagcggag atccgttaag gatctccgtg agactataga atgcctgatg cgctacgctc
    10201 atcaggcata caggacttcc gccactacat taaggaaaag ttatggtatc cgcactcatc
    10261 accgtcgccg ttatcgcctg gtgtgcgcaa ctggccttag gcggctggca aatttctcgt
    10321 tttaaccgtg ccttcgacac actatgccag caagggcggg ttggcgtgga ccgttccagc
    10381 gggcgcttta aaccgcgggt cgtggtcgcc atcgcgctgg acgatcagca gtgcatcgtc
    10441 gacaccttgt ttatgaaagg actgactgtc ttcgcccgac cgcaaaaaat tcccgcaatt
    10501 accggcatgc atgcgggtga tttacagccc gatgtgatct ttccccatga tccactatca
    10561 cagaatgctc tatcattggc gcttaaactg aaacgtggat aatttcgttg tgaatgttac
    10621 ttgcttgcga agttatcatt ttgaaaccta aatcaggtaa tcacgcccat gaaacctcgt
    10681 cagcgtcagg ccgccattct ggagtatctg caaaagcagg gtaaatgctc ggttgaagaa
    10741 ttggcgcaat actttgacac cacaggcaca accattcgca aagatctggt cattctggaa
    10801 catgccggaa ccgtcattcg tacttatggc ggagtggtgt tgaataaaga ggaatccgat
    10861 ccgcctatcg atcataaaac gctcatcaac acccacaaga aagagctgat tgcagaagct
    10921 gccgttagtt ttatccatga tggcgattcg atcattcttg atgctggcag taccgttttg
    10981 cagattgttc ccctgctctc gcgctttaat aacatcacgg tgatgaccaa cagcctgcat
    11041 atcgtcaatg cgctatccga actggataac gaacaaacta tcctgatgcc aggcggaaca
    11101 tttcgcaaaa aatcggcctc atttcacggg caactggcag agaatgcctt cgagcatttc
    11161 accttcgata aattgtttat gggcaccgac ggcatcgatc tcaatgcggg cgtaaccacg
    11221 ttcaacgagg tttataccgt cagtaaggca atgtgcaatg ccgcgcgcga agtgattttg
    11281 atggcggact catcaaagtt tggccgtaaa agccccaacg tagtttgcag tcttgaaagc
    11341 gtcgataagc taattaccga cgcaggtatc gatccggcgt ttcgtcaggc gctggaagag
    11401 aaagggatcg atgtgatcat aaccggagag agcaatgagt gaagcactac tgaacacggg
    11461 acgtcagacg ttaatgctgg agttgcagga agcaagccgt ttaccggaac gtctgggcga
    11521 tgattttgtt cgcgccgcca atatcatcct gcactgcgaa ggcaaagtgg tggtttcggg
    11581 aattggcaaa tcgggccaca ttggtaagaa aatcgccgca acacttgcca gtaccggcac
    11641 accggctttt tttgtccatc cggcagaagc actgcacggc gatctgggga tgattgaaag
    11701 ccgcgatgtg atgctgttta tctcttactc cggtggcgcg aaggaactgg atctgattat
    11761 tccgcgtctg gaagataaat ctatcgcgct gctggcgatg accggcaaac cgacgtcacc
    11821 gctgggcctg gcggcgaaag cggtgctgga tatctccgta gaacgcgaag cctgcccgat
    11881 gcaccttgcg ccgacctcca gcaccgtcaa taccctgatg atgggtgacg cgctggcgat
    11941 ggcggtcatg caggcgcgcg gttttaatga agaagatttt gcccgctccc acccagctgg
    12001 ggcactgggc gctcgcttgc tgaataaagt gcatcatctg atgcgtcgtg atgacgctat
    12061 cccacaggta gcgttaaccg ccagcgtgat ggatgcgatg ctggaactca gccgcaccgg
    12121 tctggggctg gtggcggtat gtgacgctca acaacaggta caaggcgtct ttaccgacgg
    12181 cgatttacgt cgctggctgg taggcggcgg cgcactcacc acgccagtta atgaagcgat
    12241 gacgacaggc ggcaccacat tacaggcaca aagtcgcgcc atcgacgcca aagaagttct
    12301 gatgaaacgc aaaatcactg ccgcaccagt ggtagatgaa aacggcaaac tcaccggcgc
    12361 aattaacctg caggatttct atcaggccgg gattatttaa tccttcaatc ccagacgttt
    12421 cgccagccga tgcaggttgg cgacgtcagt ttccagcatt cgcgcacagg ctgcccagtt
    12481 gtgatgattt tgtgccagtg cctggcgaat agtttcacgc tggaacgctt ctgtcgcttc
    12541 acgcaggttt tgcttaacaa cgggcaccgc cgccgcttct ggcggcggca acgtcacctc
    12601 aggaaaagca aaatgttgcg cctcaagaat cacttcatcg ccgttgcggg tggctctcgc
    12661 cagcactacc gcccgatgaa tagcatgttc cagttcgcgc acgtttcccg gaaaactgta
    12721 gtgttgcagt aaatttcgcg ccccggcact taataccacg cgggagagcc cctgccgcaa
    12781 acgacactgc tcgcagaaat accccgccag cagaatgaca tcatcgcccc gctcacgcag
    12841 cggcggcacc gaaagtggaa acacgctcag gcgatgaaac aaatcggcgc ggaatcgccc
    12901 tgccagcacc tcttcgcgta agtcgcggtt agtcgccgcc agcacgcgca catcgacccg
    12961 caaactacgg tcatcgccaa cgcgctgaat atcgccatac tgcaacaccc tcagcagctt
    13021 ggcctgcaat gccaacgaca actcgccgat ctcatcgaga aacagagtac cgttatccgc
    13081 catttcaaac ttcccgctgc ggttactgat agcgccagta aacgctcctt tcacatgccc
    13141 gaacaactca ctttccgcca cactttccgg cagtgcggca cagttgagat agaccagcgg
    13201 attcaccgcc cgtggcgagg cttcatgaat cgctttcgcc accagctcct taccggttcc
    13261 cgtctcaccg ctgatcagaa cgttgagatc ggacgccgcc acaatctcaa tctctttttt
    13321 cagttgcgtc atgccagggg acaagccaat catctgcgtc tgtttcaccg cttcaaacgg
    13381 cgtggcatcg cctggcagca tattctggct ttccagttgt tcaatcagca acgcattgct
    13441 taacgctccc gccgccagcg cggcaatcag ccgtaactct tcgtcgctga aaacatcgaa
    13501 ctgatcgggc tgcatcccgt cgagcgtcag tgcgccgatc aggttttgtc cgacaaacaa
    13561 tggcagacca acgcaggcgt gaaccttcag actctcctgc ccaggaatca aaccgtcata
    13621 gggatcgggc aattcgctgt ctgcgggaaa gcgcaccaca tccccggcgc gggcaatcgc
    13681 ttccagccgt ggatgccctt ccagcgcaaa gcgtctaccg agtacatcct ttgccagacc
    13741 gtcgatggca agcggaataa actgccgcga atcgtaacgt agcaacgcag acgcatcgca
    13801 ctccagcacc tgacgtagcg tggtgatcag gcgctgaaaa cgatcctgat gaccaatccc
    13861 acgctgcaat tcgatggcga tattcgccag cacatcaacg gaaaaactca tctttgcctc
    13921 actgtcaatt tgactataga tattgtcata tcgaccattt gattgatagt cattttgact
    13981 actcattaat gagcataatt ttatttatag agtaaaaaca atcagataag aaactggcac
    14041 gcaatctgca attagcaaga catcttttta gaacacgctg aataaattga ggttgctatg
    14101 tctattgtgg tgaaaaataa cattcattgg gtgggtcaac gtgactggga agtgcgtgat
    14161 tttcacggca cggaatataa aacgctgcgc ggcagcagct acaacagcta cctcatccgc
    14221 gaagaaaaaa acgtgctgat cgacaccgtc gaccataaat tcagccgcga atttgtgcag
    14281 aacctgcgta atgaaatcga tctggcggat atcgattaca tcgtgattaa ccatgcagaa
    14341 gaggaccacg ccggggcgct gaccgaactg atggcgcaaa ttcccgatac gccgatctac
    14401 tgtactgcca acgctatcga ctcgataaat ggtcatcacc atcatccgga gtggaatttt
    14461 aatgtggtga aaactggcga cacgctggat atcggcaacg gcaaacagct catttttgtc
    14521 gaaacaccaa tgctgcactg gccggacagc atgatgactt acctgacagg cgacgcggtg
    14581 ctgttcagta acgatgcttt cggtcagcac tactgcgacg agcatctgtt caacgatgaa
    14641 gtggatcaga cggagctttt cgagcagtgc cagcgttact acgccaatat cctgacgccg
    14701 ttcagccgcc tggtaacgcc gaaaattacc gagatcctgg gctttaactt accagtcgat
    14761 atgatagcga cctcccacgg cgtggtctgg cgcgataacc cgacgcaaat tgtcgagctg
    14821 tacctgaagt gggccgctga ttatcaggaa gacagaatca ccattttcta cgacaccatg
    14881 tcgaataaca cccgcatgat ggctgacgct atcgcccagg gaattgcgga aaccgaccca
    14941 cgcgtggcgg tgaaaatttt caacgtcgcc cgaagcgata aaaacgaaat cctgaccaat
    15001 gtcttccgct caaaaggcgt gctggtcggc acttcgacga tgaataacgt gatgatgccg
    15061 aaaatcgccg ggctggtgga ggagatgacc ggattacgct tccgtaacaa acgcgccagt
    15121 gctttcggat ctcacggctg gagcggcggt gcgatggatc gcctttccac gcgcctgcag
    15181 gatgcgggtt tcgaaatgtc gctcagcctg aaagccaaat ggcgaccaga ccaggacgct
    15241 ctggagttat gccgtgaaca cggtcgcgaa atcgcccgtc agtgggcgct cgcgccgctg
    15301 ccgcagagca cggtgaatac ggtagttaaa gaagaaacct ctgccacgac gacggctgac
    15361 ctcggcccac gaatgcagtg cagcgtctgc cagtggattt acgatccggc aaaaggcgag
    15421 ccaatgcagg acgttgcgcc aggaacgccg tggagtgaag tcccggataa cttcctctgc
    15481 ccggaatgct ccctcggcaa agacgtcttt gacgaactag catcggaggc aaaatgagca
    15541 acggcattgt gatcatcggt tcgggcttcg ccgcccgcca actggtgaaa aatattcgca
    15601 aacaggacgc cactattcca ttaaccctga ttgccgccga cagcatagat gagtacaaca
    15661 aacccgacct cagccatgtt atcagtcagg ggcaacgtgc cgacgacctt acccgccaga
    15721 cggcgggtga atttgccggg cagtttaatc tgcgcctgtt tccgcacacc tgggtgacgg
    15781 atatcgatgc cgaagcccat gtggtgaaaa gccagaataa tcagtggcaa tacgacaagt
    15841 tagtgctggc aaccggtgcc agcgcctttg tcccgcctgt gcctgggcgt gagttaatgc
    15901 tgacgttaaa tagtcagcaa gagtatcgcg cctgtgaaac gcaactgcgg gatgcccgac
    15961 gcgtgttgat tgtcggcggt ggtttgattg gtagcgaact ggcgatggat ttttgtcgtg
    16021 caggcaaagc ggtcacgcta atcgacaacg ctgccagtat tctggcgtca ttaatgccac
    16081 cagaagtaag cagccgcttg cagcatcggt tgacggagat gggcgttcat ctgctgttga
    16141 aatctcagtt gcaggggctg gaaaaaacgg attctggcat tctggcaacg ctggaccacc
    16201 agcgtagtat cgaagtggat gcggtaattg ccgccaccgg actgcgcccg gaaacagccc
    16261 tggcacgacg cgccgggctg acgattaatc gcggcgtttg cgtcgatagt tatctgcaaa
    16321 ccagtaatgc cgatatttac gcgctgggcg attgcgcgga aattaacggt caggtattgc
    16381 cgttcctgca gccgattcaa cttagcggga tggtgctggc aaaaaatctt ctcggcaata
    16441 acacgccgct gaaactaccg gcgatgctgg tgaaaatcaa aacgccggaa ttaccgctgc
    16501 atctggcagg cgaaacccag cgtcaggatt tacgctggca gattaatacc gaacgccagg
    16561 gaatggtggc gcgcggcgtt gacgatgctg accagcttcg cgcctttgtg gtcagtgagg
    16621 atcggatgaa agaggcattt gtattgttga aaacgttgcc ggtgtaggtg ggctactgtg
    16681 cctaaaatgt cggatgcgac gctggcgcgt cttatccgac ctacggggac gcatgtgtag
    16741 gccggataag gcatttacgc cgcatccggc aagggtgtcc aaatgcaaca cgctttatcc
    16801 gttctggact tcacccgcta accaacgcgc cgcagcaata accccctgcc ccagagacaa
    16861 accgccatca cccgccggta aactctgtgg aaagagcaat gtgaaatcag cgagataatg
    16921 cgccagacgt gcacgcagca aacggttatg aataaccccg ccgctaaata ccagcgtagt
    16981 gataccacgc atcgtggcct gctcacgcat caacgcggca aaaccctgcg ccagcgcatc
    17041 atgaaacgcc cacgcgcgtt gattaaccag tgcctgccag ttcagccact gctgccagaa
    17101 agtggcgaga tccagttgat tgtccacccg cggcatcgtc accggatgcg tcactccgtc
    17161 gcatgaggct gcgagcgcct ccagagcaca agccgcttca ccttcataac ttaacgtggc
    17221 tggcgcacag cccagtgccg ccgccactgc atcgaacaac cgcccacacg atgatgccag
    17281 cggcgcgtta attccacgct caatggcccg cgccagtacg ctccagtttt gctgttgcac
    17341 acttgccgtt tcagggtaat tctgccactc cggcacaaag cgcaggcact gcgccagcag
    17401 gtttcgccac ggctgcttcg ccgccaaatc gccacccgga agcgccactg caggcaagcc
    17461 gcccaggtgc tcacattcgc gatagttcac ccgcaggcac tcgccgcccc acaaagcgcc
    17521 attctccccc ataccgatac cgtcgagcgt caaagcaatg acatcaccgc catccagcgg
    17581 ccactgatgc tctgccagac acgccgctgc atgggcatga tgatgcagta ccgtttgcgt
    17641 cggcagattc atttcgctcg cccactggct ggagacatag cccggatgca catcatgcac
    17701 aatgtattgc ggagtgaaat cgtagatgtt ttgcatcagg cgtaacgctt cgcgccactg
    17761 catctggatg ccatcgtcac ttaaatcgcc cagatgctga ctcaacaccg cttgttcacc
    17821 gcgcaccagg cagaaggtat ttttcagatc cgcgccaaga cacagcacag gcggaacatt
    17881 tttaaagccc ggaggcaaaa ccagcgcatc cggcacatac ccccgcgaac ggcgcagcat
    17941 ttcgccgctt tcgcgcacca ctgaatcatc catccgctgc acgatgtcgc ggttatgtag
    18001 caagaatccg tcggcaatgc cctgcaaatc cgccagcgcc tgttcgttgc tgatagctgg
    18061 tggtttaccg ctcaggttgc cggaggtcat caccagcggg cattgcagtt cctgtaacag
    18121 caaatgctgg agcgggttcg caggcaacat caccccgact tcgttaaggc caggggcgat
    18181 atcatcacaa agctcaggta cgtatttttt atccaccagc acaatcggcg cagcgggcgt
    18241 ggtaagcaac tggcgcgcag cgtctggtaa actttctgcc actggcaaca tgactgccag
    18301 cggtttcgcc gggcgatgtt tgcgctcccg cagtgtcgcc accgcgttac tgttacgtgc
    18361 atcgcaggca agatgaaatc cgccaatccc tttgatggcg acaatgttgc ccatttttaa
    18421 ctgtgcgata gctgcctgta atgccgcctc ttgttctgca tgttcaccat gacttaccca
    18481 ttcaagatgc gggccacact ccgggcaggc caccggctgg gcgtggaagc gacgatcgag
    18541 cgggtcgcgg tactctttgt cacaggccgg acatagcgga aacgccgcca tcacggtaaa
    18601 cgggcggtcg taaggcatcg cgcgaataat ggtgaaacgc gggccgcagt gggtacagtt
    18661 gataaacgga taacgataac gccgctcgcc tggggtattc atttcggcaa ggcaagcagg
    18721 gcaagtggcg gcatccggga caatttgcgt attcatggtg ccgccggtgc tctggcggat
    18781 ggtgaactcg gtgggcagtt gtgaccagat aaacggctca cgctcgacgc tatcaatacg
    18841 cgccagtggc ggacagtgct gatgcaattg aacaagaaac gtttccgggt cttccagcag
    18901 ccggacttct acgccatcgc cgtcattaca tacatcgccg tgaagattta attgctgtgc
    18961 cagctgccag acaaacggac gaaaaccgac gccctgcact ttgccacgaa tacgcagttg
    19021 gacaccgcaa gatgtgtttt ttgccattga gttattcccg ccatcatgaa ttgcgtaacc
    19081 cgccctgccg gacacgacag cgtcgtatcc ggcagtctca ggtcggcgat accgcccgct
    19141 ccgtattcta cgaatatttc cgggaattcc tttgatgcca gaacagttct gtaagatttt
    19201 tagaacatca gcgccgtacg gcggcgtttt tctgcgctca gttgttcaag tttattacga
    19261 tcgacacaaa tcagcgcatg ggtcgggcaa gccgccatac acgccgggcc gtcttcacga
    19321 tggttgcaca ggtcgcattt attggcttcg gctttgtcag cccgtacatt cagacccgcg
    19381 ccgctgttgc gaatcacagg acgtaccacc acttccatcg cgccatacgg gcaagccacc
    19441 acgcaggttt tgcaaccaat gcaacgttcc tgcatcacag gaacaaaccc tttatcacgg
    19501 ctgatagcac cattcgggca gacgttagcg cacggtgcat cttcacactg acggcaaact
    19561 gtcgccgtgg aaatgttcac acctttaatg acatggatac gcggtaaaaa tgtttccggg
    19621 gtcagcgatg cacagtcctg attttcctga tgagaaacca cgcacgccac ttcacaggta
    19681 cggcaaccaa tacatttact cgcgtcagca atgatgaaac ggttcatcaa attctccagc
    19741 aatgacagtt aatgcgccga tacactcaca aatcatgcca gtttttaatt tgctgttatt
    19801 taaggaaatt aatttctgta atgcaggaaa aaagatgtca tcgacactag tgacgatgac
    19861 atgtgatgac aatgtttatc gcgaaagggc aatgagtgag tcgcggcgga tcagttttcc
    19921 gctaaaggtt ttcggcggtg agaaatcccc gccatcgagc ataaaaatca gccgtccaat
    19981 aatttcctga atcatctcag tcaccggaat ttttacgctg gagagcgccg gaacgatata
    20041 gggggcaatt gcgatatcat cgaatccgat aactgacacc tgctctggca ccgctacgcc
    20101 gcgctcgtgc aacgctttga tcgcacctat cgccatatcg tcgttactgg caactaacgc
    20161 gctaaattta gcccctcgtt cgagcaacgt ttctacccct tcggccccgc tggcgggcgt
    20221 ccatttaccg ttagcgataa gtttttcatt gagcgcaata ccatgctgcg acagcgcgtc
    20281 tttatacccg gcaagacgtt caatgctggt gggggaatcc atcgagccgg taaggaaagc
    20341 aatctcctga tgcccggcgt ttatcaactc tgccacggcg ttaaagctgg tctgtttatg
    20401 atcgcaccag acgctatggc tgctattatt gcgcaggcgg cgattaagca ccattatcgg
    20461 ctgactgtgc gcgtcaatga tgtcatcaat ctcatccacg cttaaaaaac gcgggtaaat
    20521 cataatggcg tcgcagcgca aatccagcag atactgaatc gcctggcgct cttcttcggc
    20581 gctgtgttta ccatctgcca atagcaactg tcgccctttc tcttccgcca ttcgcgcggc
    20641 atgaaacagt aattcactaa aataaatgcc gtgataaagc gtgttggtca ctaccagccc
    20701 cagcgtctga gtactcttcg ccgacagatt gcgggccagc aaatttgggc ggtaactgct
    20761 ctcttctacc gcctgaaaca cgcgatcttt agtctcctgg ctgacgtagc cattacctga
    20821 aagcacgcgg gaaacggtcg cttttgaaac cccggcgcgc ttcgccactt ccagcatcgt
    20881 cgtcatcatt ttcatccctt tacacgcaat caacgcagtg tactgcaccg tttgccgatt
    20941 gtccttgcgc aatcggctcc agcggataag acgaggtcgc cgtgccggtt ttgatgactt
    21001 ttttgataaa ggtaaacatg gcgattcctt atttcagcgg cgagttttta cgctcaatgc
    21061 tgtaacgctc gagttctttg tacggcacca ctttgctctt cttcttacgc acatcgacta
    21121 cggtcatgcg gtcggtacag gagtagcaag ggtccaggct accgataatc agcggcgcat
    21181 aggaaacggt attgccacgc agcatgtagc gcagggtcgg ccagttcgcg taggtcgcgg
    21241 cacggcagcg ccagcggtac agcttctggt tgtcgccggt catgctccag tggatatcgt
    21301 cgccgcgcgg cgcttcggca aagcccagcg cgaaacggtg cggaatgtag gtaaagcctt
    21361 ccaccatcag cggaccgccc ggcaggttat ccagaccgta gtcgatcata ttcagcgcgg
    21421 tatagacctc gttgattcgc actttcagac gggaaataac gtcgcagccc tgctcgctgt
    21481 ggacttccat tggcagcagg ccataaccga caaacgggtg atcggcgcga gtatcgcggg
    21541 cgagaccgct ggcgcgaacc atcgggccaa cgttactgaa atcgcgggcg atttccgggt
    21601 ccagacgacc aatgccgacg gtgcgttgtt ccatgttcgg cgtactcagc agcacatcca
    21661 ccagctcctg cacttcacga cgcatttgtt gcgccagctg gcgggtctgg atcatgtcat
    21721 ctttcagcag atcgcgacga atcccgccga tcaggttcag gccgtaggtt ttacgcgcac
    21781 cggtaaggat ctctgccatt ttcatggagg tttcacgcac gcggaagaac tgcataaagc
    21841 cggagtcgaa gccggtaaag tgacaggcga ggccgaggtt gagcagatgc gggtgcaggc
    21901 gttccacctc cagcagaatg gcgcggatca tctgcgcgcg ttctggcacc tgaataccca
    21961 tcgcgttttc caccgacgtg gtgtaggcgg tgctgtgggc aaagccgcag atcccgcaca
    22021 cacggtcaga gaggaaggtc acttcgttat aacccatgcg ggtttccgcc agtttttcca
    22081 taccgcgatg gacgtagaac agacggtagt cggcgtcgat aatgttttcg ccatcgacga
    22141 acagacggaa gtgacccggt tcgtcagaag tgacgtgcag cggaccaatc ggcacgacgt
    22201 tgtttttctt gtcgcccagt tcgttgatga actcgtaggt ttcagcatcg gtagtcggtg
    22261 ccggacgctg acgataatcc atgctgtctt tacgcagcgg ataaagttca tccggccagt
    22321 catccggcag caccagacga cgttcatccg gcagaccaac cggaatcaaa ccgtacatat
    22381 cgcgcacttc gcgctcgccc cacaccgccg ccggaacgcg cggcgtcacg gacggatact
    22441 ccggtttgtt ggcgtcaact tcgacgcgaa ccgttaccca acacttggtg cccttctcca
    22501 tcgacagcac gtaataaacg gcgtaatgac cattcagttt gcgttcgtcg ttaccaaaca
    22561 gcaccgacag ccagccaccc tgcttgtagt agagaaactc caccacttcc ggcagatagt
    22621 tcacctttac ggtgacagtc agctgatctt tggtctgcca ggcgtggtcc agcacgacgc
    22681 ctagaaatgc ctcattcagc gcggcgagat aatgttgacc taatttttct tcagacatgc
    22741 tcaaactctc tttaatcacg ccgccagcaa ggagacgaac gctaaaaatg caaagccaaa
    22801 cccagcccag gtaatgcgcg gagtaatatc aagacgcaga cgggccatgc tgttttcgaa
    22861 cagcgcgata accaggacgc cgaccaccag tttgacgatg gcaatcacca gcgccagcag
    22921 cagtccaccg acggtgaagg tttccatttg tccccacgga ataaacaccc cgacgaacat
    22981 ctgcaacacc accagctgtt tcaggctgat accccatttc atgacgccaa agccgctacc
    23041 gctgtattcg gagagcggac cttcctgcaa ctcctgctcg gcttccgcca ggtcgaacgg
    23101 cagtttgccc atttcgataa aagtggcgaa cgcacaggcg cagagcgcca gcaccagcgg
    23161 gatgctttgg ctcagcggcc agtgataaac ggtgtcggta atgttgctga tgttggtgga
    23221 accggcaacc tgtgcggcaa cccacagacc aagcagcaac atcggttcga ccagcacgcc
    23281 aagcatcgct tcacggctcg caccgatagc ggtaaacggg ctaccggtat ccagaccgga
    23341 aatggcaaag aagaaacgcg cgatggcgaa gagatacagt aaggtgatca aatcgcccag
    23401 ttgcggcagc ggagaaccga cggtcaccac cggcagcgca gtagcgatag tcagcatgac
    23461 gcccaccatc acatacggcg tcaggcggaa catccagcca gaggcatccg ggccaacgct
    23521 ctggcggccc agcagtttga taatgtcgcg atactcctgc aacacgcccg gcccgcgacg
    23581 gttatgcaga cgggcgcgcg ccacgcgggt aataccggag agcagcggcg caacggcaaa
    23641 taacaccagc gcctgaatta acggatataa aacactcatt ctcaggctcc tcgtgaaaca
    23701 ataatcacca ccagtaccgc cagttcaacc agcgccatcc ggcggaacag caacgcacta
    23761 ccttcgcact gccagcccgg cactagcgac accggattca gccatttgcg cagtttcagc
    23821 actggcgcaa acgcctgttt aaccggcatg gcaaaaccgt gagcggtaat caccattgat
    23881 ttttcatggt cgtagccgca cacccaggcc gcaccgcggg aacgtgacgg caggcggtcg
    23941 cctttgcaaa tcgccataat gatgaatggc agcagcgggc aggcaatcag cagcaatgtg
    24001 atcattggtt gagaaacggt ggtgttagca ggctccagag gcagaggtac agcagcagag
    24061 agcatcggca gcagccacgg cgcagcaaca ccgccaatta cgcagcaaat cgccagtgcc
    24121 actacgctta cgctcatcag gagcggcgca caggtggcgt tttcggcttc tttggtacgc
    24181 ggcgcgccga ggaacgtgac gccatagact ttcgccatac acatcaccgc cagcgcaccg
    24241 gtaattgcca gacccacagc gagcagcggc cccagcagac gggcaacaaa cgcgccgcta
    24301 ttgctcagtt tgaagaagga ttgatagata acccattccc cggcaaaccc gttcagtggc
    24361 ggcagcgcag ccattgccat caacccgact aacatggcga tggagataac cggcattttc
    24421 ttgccaatac caccgagttt ttcgatatcg cgatgaccgg tacggaacca gacgctcccc
    24481 gccccgagga acagtacgct tttgaacagg ctatggttaa gcagatggta cagaccaccg
    24541 accaggccaa gagcaatcag cgccggttgt tcgagcgcga tacccgttac gccagcgccc
    24601 agccccagcc ccagccccag ccccagcccc agccccagca ggataatgcc gatattttcc
    24661 agggtgtggt aagccagcag gcgctggatg ttgtgttcca tcaacgcata cagaccaccg
    24721 acgaacgcgg tgatcatgcc gagcaccagc agcgcgatcc cccaccacag cggtgcatta
    24781 ccgcccagca gtgacagggt taacatgccc agcaggccaa ttttcatgac taccgtagaa
    24841 aacaacgcgg cagctggcgc agaggcgtta gcatgtgcct gcggcaccca gccgtgcagc
    24901 ggaataatcc cagccagcag gccaaagcca atcacaccga gcagccagat atcggaaccg
    24961 agcggcagct gttgcatacg catatccagc aggcgcagat ccagcgtgcc ataacgctgc
    25021 cacagcagcc agcaagcaat caccagcagc agagtgccaa gacgcctcag cgcaaaccac
    25081 agtttgccct ctttgctgtt gctggtgagg aacaccgcac acaaggccat gacttcggcc
    25141 attaccacga acatgccgag gttgctggcg atgacggcgc agacggcggc agccatcaac
    25201 atattgatct gcaagccgtt gcacttcacc tgcgcgtggc gatgccagtc aatgttatag
    25261 aggctgacaa acagaccgca aaggccgagg gtaatcagcc aaatcgcgtt aagcggggag
    25321 atttgcacat cgtagcttac cagcgacagc gcaccgctca cgccaaccgc gccagtcagt
    25381 acagtgaagc ccgcggctgc cgtatacaga ctaccaaccg cgccgccaat tccggctatc
    25441 cagccactta gcgctttttg aaaagagaaa tgccagaaca gcggcggcga caaaccatgc
    25501 cacgccgcta ttgatcaggg aaattgcgct catttagcct ctccactttg agcctgctga
    25561 aacaaggtga gatcgccaaa gtccgtgtta aaggtcagct cacgcttacg tttgctgacg
    25621 cgggcgatat cggtgttatc caccagatgc agggctttag tcgggcacat ccgcacacag
    25681 gccggacctt gttcatcaaa gctacaaagg tcacatttga cggcgatcgc gcgaatacct
    25741 ggcacccagt caagcaatgt gctgacacgc gccggagcag gcggtgccgg tggcgctttc
    25801 ggggtattgg cgtttgccgg aatatccagc ggacggctgc cggaaaattc aattgcgcca
    25861 aacgggcagg cgatgccgca cagtttgcag cttacgcaca gactttcatt caactgcacg
    25921 gccccatcaa cgcgggtgat ggcgttaacc gggcagacca ccgcgcaggg tgcatcttca
    25981 cagtggtgac agagctgcgg cgcagattct ttttcattga gcatcactct caggcgcggc
    26041 attgattgca ggccgtgctg gcgatgcgtc tctgaacagg cggcctcaca ggtgtggcag
    26101 ccgatacaga gcgtggagtc ggcaattaca aaacgattca ccaggcattc ctcaggtgat
    26161 tgtcattttt gacgaaaaca tgccgatgaa atgtcatttt cgacactcat cgacacgccc
    26221 atccccaaac aggcgtaacg cctgcaaaac gggcaaagcc tcagctcatg ctgccgggct
    26281 ttgtcccttt accagttggc ttaaattcac cggcacattg ttttcaatgg cggtgtataa
    26341 gctgtcgtaa acgccagcga ttttttcgag atagtgttcc agaacctgtt cgcgatcgcg
    26401 gatgttgacc ttgccatcaa tcatgccgtc aatgctcagt tgcgcctgcg caatccgctg
    26461 tttgtcttcg ccatcttttg tttcgacgta atactcgatg gtgtggctgt taaagccatc
    26521 cgccagggtg acgtaaatac gaaaatgttc aaaaaggacg aaacagagtt ctttgtccgg
    26581 cgcgcagctc atggcgtgat gcaggcgcgc tttcgataac gtgatcccct gaaccagcga
    26641 attgcagtag atgtgccact ggtcctgtag gcgacgatgc cgctgtgcga tgtaatcggc
    26701 tttctcgctt atttcccaaa tagtcattgt caggttactc gtttaacaga gatgccagct
    26761 ttaagcattt tttgtgccaa cttttaattt attgttatta aagagatttt taagctaaag
    26821 atgaatttcg tcgccgtgtc gacgtgtcat ttcgacatca tcgacattat tcaccgcagg
    26881 gataatcaac actggcgcaa ttattgcttg tagctggcaa tagttaatgg gaggcgatat
    26941 gcacgaaata accctctgcc aacgggcact ggaattgatc gaacagcagg ccgcaaaaca
    27001 cggcgcaaaa cgcgtaactg gggtctggct caaaattggc gcattttctt gtgtcgaaac
    27061 cagctctctt gccttttgtt ttgatctggt ttgccgcggc agcgtggcgg aaggttgtaa
    27121 actgcacctc gaagaacaag aggccgaatg ctggtgtgaa acatgccaac agtatgtgac
    27181 gctactgacc cagcgcgtcc gccgctgtcc acagtgtcat ggtgacatgc tacagattgt
    27241 ggcagacgac ggtttacaga ttcggcggat agaaatagac caggagtgag cgatgtgtac
    27301 aacatgcggt tgcggtgaag gcaacctgta tatcgagggt gatgaacata accctcattc
    27361 cgcgtttcgt agcgcgccat ttgccccggc ggcacgcccg cagatgaaaa tcaccggcat
    27421 taaagcgcct gaatttaccc ccagccagac tgaagaaggc gacctgcatt acggtcatgg
    27481 cgaagcgggc actcacacac cgggcatgag ccagcgtcgg atgctggaag tcgaaattga
    27541 cgtgctggac aaaaacaacc gtctggctga acgcaaccgc gcgcgctttg ctgcccgcaa
    27601 gcaactggtg ctcaacctgg tttccagccc tggttccggt aaaaccaccc tgctgacgga
    27661 aaccttaatg cgcctgaaag acagcgttcc gtgcgcggtt attgaaggcg accagcaaac
    27721 cgtgaacgat gccgcacgca ttcgcgctac cggtacacca gcgattcagg tgaacaccgg
    27781 taaaggctgc catcttgacg cacagatgat tgccgacgcc gcaccgcgtc tgccactgga
    27841 cgataacggt attctgttta tcgaaaacgt tggcaacctc gtatgcccgg ccagcttcga
    27901 tctcggtgaa aaacacaaag tagcggtgct ttccgttacc gaaggtgaag acaaaccgct
    27961 gaaatatccg catatgtttg ccgccgcctc gctgatgctg ctcaacaaag ttgacctgtt
    28021 gccgtatctc aactttgacg ttgagaagtg catcgcctgc gcccgcgaag tcaatccaga
    28081 aattgaaatc atccttattt ccgccaccag cggcgaaggg atggaccagt ggctgaactg
    28141 gctggagaca cagcgatgtg cataggcgtt cccggccaga tccgcaccat tgacggcaac
    28201 caggcgaaag tcgacgtctg cggcattcag cgcgatgtcg atttaacgtt agtcggcagc
    28261 tgcgatgaaa acggtcagcc gcgcgtgggc cagtgggtac tggttcacgt tggctttgcc
    28321 atgagcgtaa ttaatgaagc cgaagcacgc gacactctcg acgccttaca aaacatgttt
    28381 gacgttgagc cggatgtcgg cgcgctgttg tatggcgagg aaaaataatg cgttttgttg
    28441 atgaatatcg cgcgccggaa caggtgatgc agttaattga gcatctgcgc gaacgtgctt
    28501 cacatctctc ttacaccgcc gaacgccctc tgcggattat ggaagtgtgt ggcggtcata
    28561 cccacgccat ttttaaattc ggcctcgacc agttactgcc tgaaaacgtt gagtttatcc
    28621 acggtccggg ttgcccggtg tgcgtactgc cgatgggtag aatcgacacc tgcgtggaga
    28681 ttgccagcca tccggaagtc atcttctgta cctttggcga cgccatgcgc gtaccaggga
    28741 aacagggatc gctgttgcag gcaaaagcac gcggtgccga tgtgcgcatc gtttactcgc
    28801 cgatggatgc gttgaaactg gcgcaggaga atccaacccg caaagtggtg ttcttcggct
    28861 taggttttga aaccaccatg ccgaccaccg ccatcactct gcaacaggcg aaagcgcgtg
    28921 atgtgcagaa tttttacttc ttctgccagc acattacgct tatcccgacg ctgcgcagtt
    28981 tgctggaaca gccggataac ggtatcgacg cgttcctcgc gccgggccac gttagtatgg
    29041 ttatcggcac tgatgcctat aattttatcg ccagcgattt tcatcgtccg ctggtggtcg
    29101 ctggtttcga accccttgat ctactgcaag gcgtggtcat gctggtggag cagaaaatag
    29161 cggcccacag caaggtagag aatcagtatc gtcgggtggt accggatgcc ggtaacctac
    29221 tggcgcaaca ggcgattgcc gatgtgttct gtgtcaacgg cgacagcgaa tggcgcggct
    29281 taggcgtgat tgaatcttct ggcgtgcacc tgacgccgga ttatcaacga ttcgatgccg
    29341 aagcacattt ccgcccggca ccgcagcagg tctgcgatga cccgcgcgcg cgttgtggtg
    29401 aggtattaac gggcaaatgt aagccgcatc aatgcccgct gtttggtaac acctgtaatc
    29461 ctcaaaccgc atttggtgca ctgatggtct cctccgaagg agcgtgcgcc gcgtggtatc
    29521 agtatcgtca gcaggagagt gaagcgtgaa taatatccaa ctcgcccacg gcagcggcag
    29581 ccaggcgatg cagcaattaa tcaacagcct gtttatggaa gcctttgcca acccgtggct
    29641 ggcagagcag gaagatcagg cacgtcttga tctggcgcag ctggtagcgg aaggcgaccg
    29701 tctggcgttc tccaccgaca gttacgtcat tgacccgctg ttctttcctg gcggtaatat
    29761 cggcaagctg gcgatttgcg gcaccgcgaa tgacgttgcg ggcagtggcg ctattccgcg
    29821 ctatctctcc tgtggcttta tcctcgaaga aggattgccg atggagacac tgaaagccgt
    29881 agtgaccagc atggcagaaa ccgcccgcac ggcaggcatt gccatcgtta ctggcgatac
    29941 taaagtggtg cagcgcggcg cggcagataa actgtttatc aacaccgctg gcatgggcgc
    30001 aattccggcg aatattcact ggggcgcaca gacgctaacc gcaggcgatg tattgctggt
    30061 tagcggtaca ctcggcgacc acggggcgac tatccttaac ctgcgtgagc agctggggct
    30121 ggatggcgaa ctggtcagcg actgcgcggt gctgacgccg cttattcaga cgctgcgtga
    30181 cattcccggc gtgaaagcgc tgcgtgatgc cacccgtggt ggtgtaaacg cggtggttca
    30241 tgagttcgcg gcagcctgcg gttgtggtat tgaaatttct gaatcagcgc tgccggttaa
    30301 acctgccgtg cgcggcgttt gcgaattgct gggactggac gccctgaact ttgccaacga
    30361 aggcaaactg gtgatcgccg ttgaacgcaa cgcggcagag caagtgctgg cagcgttaca
    30421 ttcccatcca ctggggaaag acgcggcgct gattggtgaa gtggtggaac gtaaaggtgt
    30481 tcgtcttgcc ggtctgtatg gcgtgaaacg aaccctcgat ttaccacacg ccgaaccgct
    30541 tccgcgtata tgctaataaa attctaaatc tcctatagtt agtcaatgac cttttgcacc
    30601 gctttgcggt gctttcctgg aagaacaaaa tgtcatatac accgatgagt gatctcggac
    30661 agcaagggtt gttcgacatc actcggacac tattgcagca gcccgatctg gcctcgctgt
    30721 gtgaggctct ttcgcaactg gtaaagcgtt ctgcgctcgc cgacaacgcg gctattgtgt
    30781 tgtggcaagc gcagactcaa cgtgcgtctt attacgcgtc gcgtgaaaaa gacaccccca
    30841 ttaaatatga agacgaaact gttctggcac acggtccggt acgcagcatt ttgtcgcgcc
    30901 ctgatacgct gcattgcagt tacgaagaat tttgtgaaac ctggccgcag ctggccgcag
    30961 gtgggctata ccccaaattt ggtcactatt gcctgatgcc actggcggcg ggagggcata
    31021 tttttggtgg ctgtgaattt attcgttatg acgagcgtcc ctggagcgaa aaagagttca
    31081 atcgtctgca aacatttacg cagatcgttt ctgtcgtcac cgaacaaatc cagagccgcg
    31141 tcgtcaacaa tgtcgactat gagttgttat gccgggagcg cgataacttc cgcatcctgg
    31201 ttgccattac caacgcggtg ctttcccgcc tggatatgga cgaactggtc agcgaagtcg
    31261 ccaaagaaat ccattactat ttcgacattg acgatatcag cattgtctta cgcagccacc
    31321 gtaaaaacaa actcaacatc tactccactc actatcttga taaacaacat cccgcccacg
    31381 aacagagcga agtcgatgaa gccggaaccc tcaccgaacg cgtgttcaaa agtaaagaga
    31441 tgctgctgat caatctctac gagcgggacg atttagcccc ctatgaacgc atgttgttcg
    31501 acacctgggg caaccagatt caaaccttgt gcctgttacc gctgatgtct ggcgacacca
    31561 tgctgggcgt gctgaaactg gcgcaatgcg aagagaaagt gtttaccact accaatctga
    31621 atttgctgcg ccagattgcc gaacgtgtgg caatcgctgt cgataacgcc ctcgcctatc
    31681 aggaaatcca tcgtctgaaa gaacggctgg ttgatgaaaa cctcgccctg accgagcagc
    31741 tcaacaatgt tgatagtgaa tttggcgaga ttattggccg cagcgaagcc atgtacagcg
    31801 tgcttaaaca agttgaaatg gtggcgcaaa gtgacagtac cgtgctgatc ctcggtgaaa
    31861 ctggctcggg taaagagctg attgcccgtg cgatccataa tctcagtggg cgtaataatc
    31921 gccgcatggt caaaatgaac tgcgcggcga tgcctgccgg attgctggaa agcgatctgt
    31981 ttggtcatga gcgtggggct tttaccggtg ccaacgccca gcgcattggt cgttttgaac
    32041 tggcggataa aagctctctg ttcctcgacg aagtgggcga tatgccactg gagttacagc
    32101 cgaagttgct gcgtgtattg caggaacagg agtttgaacg tctcggcagc aacaaaatca
    32161 ttcagacgga cgtacgttta attgccgcga ctaaccgcga tctgaaaaaa atggtcgccg
    32221 accgagagtt ccgtagcgat ctctattacc gcctgaacgt attcccgatt cacctgccgc
    32281 cactacgcga gcgtccggaa gatattccgc tgctggcgaa agcctttacc ttcaaaattg
    32341 cccgtcgtct ggggcgcaat atcgacagca ttcctgccga gacgctgcgc accttgagca
    32401 acatggagtg gccgggtaac gtacgcgaac tggaaaacgt cattgagcgc gcagtattgc
    32461 taacacgcgg caacgtgctg cagctgtcat tgccagatat tgctttaccg gagcctgaaa
    32521 cgccgcctgc cgcaacggtt gtcgcccagg agggcgaaga tgaatatcag ttgattgtgc
    32581 gcgtgctgaa agaaaccaac agcgtggttg ccgggcctaa aggcgctgcg caacgtctgg
    32641 ggctgaaacg cacgaccctg ctgtcacgga tgaagcggct gggaattgat aaatcggcat
    32701 tgatttaact gcaaattgcc ggacagatct gcctgtccgg catactattc acgaggtttt
    32761 ttcggacgat atttttccgg cagttctggc accggacgct tgtcatcgat gagatgacgc
    32821 acggttaaga tcggatgacg ccacagcatt ctcggcccgg cccaacgcat aatctgtttc
    32881 atctcttcac gctttgcagg ctggtaacag tgcaccggac actgcttaca ggctggtttc
    32941 tcttcgccga acacacattt atccagccgc ttttgcgcgt aaacaaacaa cgcctcgtaa
    33001 tgctccggct ccgctgacgc ctgcgggcat ttcgcttgat aaagatcgat cattttttta
    33061 atcgtcagtt tttcacgaga gatacgcttg ccggacatgc tgcctccacc tcattaagat
    33121 gcatttatat tacatcttaa t
//
LOCUS       DAJTRC010000041        31859 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.041, whole genome shotgun sequence.
ACCESSION   DAJTRC010000041 DAJTRC010000000
VERSION     DAJTRC010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 31859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.041"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(1..400)
                     /locus_tag="OO357_002024"
                     /pseudo
     CDS             complement(1..400)
                     /locus_tag="OO357_002024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            534..1811
                     /gene="waaA"
                     /locus_tag="OO357_002025"
     CDS             534..1811
                     /gene="waaA"
                     /locus_tag="OO357_002025"
                     /EC_number="2.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid IV(A) 3-deoxy-D-manno-octulosonic acid
                     transferase"
                     /protein_id="HCR5886980.1"
                     /translation="MLELLYTALLYLIQPLIWIRLWVRGRKAPAYRKRWGERYGFYRH
                     PLKPGGIMLHSVSVGETLAAIPLVRALRHRYPDLPITVTTMTPTGSERVQSAFGKDVQ
                     HVYLPYDLPDALNRFLNKVDPKLVLIMETELWPNLIAALHKRKIPLVIANARLSARSA
                     AGYAKLGKFVRRLLRRITLIAAQNEEDGARFVALGAKNNQVTVTGSLKFDISVTPQLA
                     AKAVTLRRQWAPHRPVWIATSTHEGEESVVIAAHQALLQQFPNLLLILVPRHPERFPD
                     AINLVRQAGLSYITRSSGEVPSTSTQVVVGDTMGELMLLYGIADLAFVGGSLVERGGH
                     NPLEAAAHAIPVLMGPHTFNFKDICARLEQASGLITVTDATTLAKEVSSLLTDAVYRS
                     FYGRHAVEVLYQNQGALQRLLQLLEPYLPPKTH"
     gene            1819..2298
                     /gene="coaD"
                     /locus_tag="OO357_002026"
     CDS             1819..2298
                     /gene="coaD"
                     /locus_tag="OO357_002026"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HCR5886981.1"
                     /translation="MQKRAIYPGTFDPITNGHIDIVTRATQMFDHVILAIAASPSKKP
                     MFTLEERVALAQQATAHLGNVEVVGFSDLMANFARNQHATVLIRGLRAVADFEYEMQL
                     AHMNRHLMPELESVFLMPSKEWSFISSSLVKEVARHQGDVTHFLPENVHQALMAKLA"
     gene            complement(2337..3146)
                     /gene="mutM"
                     /locus_tag="OO357_002027"
     CDS             complement(2337..3146)
                     /gene="mutM"
                     /locus_tag="OO357_002027"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HCR5886982.1"
                     /translation="MPELPEVETSRRGIEPHLVGATILHAVVRNGRLRWPVSEEIYRL
                     SDQPVLSVQRRAKYLLLELPEGWIIIHLGMSGSLRILPEELPPEKHDHVDLVMSNGKV
                     LRYTDPRRFGAWLWTKELEGHNVLAHLGPEPLSDDFNGEYLHQKCAKKKTAIKPWLMD
                     NKLVVGVGNIYASESLFAAGIHPDRLASSLSLAECELLARVIKAVLLRSIEQGGTTLK
                     DFLQSDGKPGYFAQELQVYGRKGEPCRVCGTPIVATKHAQRATFYCRQCQK"
     gene            complement(3244..3411)
                     /gene="rpmG"
                     /locus_tag="OO357_002028"
     CDS             complement(3244..3411)
                     /gene="rpmG"
                     /locus_tag="OO357_002028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010847629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HCR5886983.1"
                     /translation="MAKGIREKIKLVSSAGTGHFYTTTKNKRTKPEKLELKKFDPVVR
                     QHVIYKEAKIK"
     gene            complement(3432..3668)
                     /gene="rpmB"
                     /locus_tag="OO357_002029"
     CDS             complement(3432..3668)
                     /gene="rpmB"
                     /locus_tag="OO357_002029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008457442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HCR5886984.1"
                     /translation="MSRVCQVTGKRPVTGNNRSHALNATKRRFLPNLHSHRFWVESEK
                     RFVTLRVSAKGMRVIDKKGIDTVLAELRARGEKY"
     gene            complement(3885..4553)
                     /gene="radC"
                     /locus_tag="OO357_002030"
     CDS             complement(3885..4553)
                     /gene="radC"
                     /locus_tag="OO357_002030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HCR5886985.1"
                     /translation="MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTL
                     AKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQLKGIAELARRYYNVRMREESPLL
                     SPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
                     KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAE
                     RGWI"
     gene            4725..5945
                     /gene="coaBC"
                     /locus_tag="OO357_002031"
     CDS             4725..5945
                     /gene="coaBC"
                     /locus_tag="OO357_002031"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418096.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HCR5886986.1"
                     /translation="MSLAGKKIVLGVSGGIAAYKTPELVRRLRDRGADVRVAMTEAAK
                     AFITPLSLQAVSGYPVSDSLLDPAAEAAMGHIELGKWADLVILAPATADLIARVAAGM
                     ANDLVSTICLATPAPVAVLPAMNQQMYRAAATQHNLEVLASRGLLIWGPDSGSQACGD
                     IGPGRMLDPLTIVDMAVAHFSPVNDLKHLNIMITAGPTREPLDPVRYISNHSSGKMGF
                     AIAAAAARRGANVTLVSGPVSLPTPPFVKRVDVMTALEMEAAVNASVQQQNIFIGCAA
                     VADYRAATVAPEKIKKQATQGDELTIKMVKNPDIVAGVAALKDHRPYVVGFAAETNNV
                     EEYARQKRIRKNLDLICANDVSQPTQGFNSDNNALHLFWQDGDKVLPLERKELLGQLL
                     LDEIVTRYDEKNRR"
     gene            5923..6381
                     /gene="dut"
                     /locus_tag="OO357_002032"
     CDS             5923..6381
                     /gene="dut"
                     /locus_tag="OO357_002032"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000976070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HCR5886987.1"
                     /translation="MMKKIDVKILDPRVRKEFPLPTYATSGSAGLDLRACLDDAVELA
                     PGDTTLVPTGLAIHIADPSLAAMMLPRSGLGHKHGIVLGNLVGLIDSDYQGQLMISVW
                     NRGQDSFTIQPGERIAQMIFVPVVQAEFNLVEDFDATDRGEGGFGHSGRQ"
     gene            6488..7084
                     /gene="slmA"
                     /locus_tag="OO357_002033"
     CDS             6488..7084
                     /gene="slmA"
                     /locus_tag="OO357_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000818604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid occlusion factor SlmA"
                     /protein_id="HCR5886988.1"
                     /translation="MAEKQTAKRNRREEILQSLALMLESSDGSQRITTAKLAASVGVS
                     EAALYRHFPSKTRMFDSLIEFIEDSLITRINLILKDEKDTTARLRLIVLLLLGFGERN
                     PGLTRILTGHALMFEQDRLQGRINQLFERIEAQLRQVLREKRMREGEGYTTDETLLAS
                     QILAFCEGMLSRFVRSEFKYRPTDDFDARWPLIAAQLQ"
     gene            complement(7121..7762)
                     /gene="pyrE"
                     /locus_tag="OO357_002034"
     CDS             complement(7121..7762)
                     /gene="pyrE"
                     /locus_tag="OO357_002034"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709421.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HCR5886989.1"
                     /translation="MKSYQRQFIEFALSKQVLKFGEFTLKSGRKSPYFFNAGLFNTGR
                     DLALLGRFYAEALVDSGIEFDLLFGPAYKGIPIATTTAVALAEHHDLDLPYCFNRKEA
                     KDHGEGGNLVGSALQGRVMLVDDVITAGTAIRESMEIIQANGATLAGVLISLDRQERG
                     RGEISAIQEVERDYNCKVISIITLKDLIAYLEEKPEMAEHLAAVKAYREEFGV"
     gene            complement(7828..8544)
                     /gene="rph"
                     /locus_tag="OO357_002035"
     CDS             complement(7828..8544)
                     /gene="rph"
                     /locus_tag="OO357_002035"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006803115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HCR5886990.1"
                     /translation="MRPAGRSNNQVRPVTLTRNYTKHAEGSVLVEFGDTKVLCTASIE
                     EGVPRFLKGQGQGWITAEYGMLPRSTHTRNAREAAKGKQGGRTMEIQRLIARALRAAV
                     DLKALGEFTITLDCDVLQADGGTRTASITGACVALADALQKLVENGKLKTNPMKGMVA
                     AVSVGIVNGEAICDLEYVEDSAAETDMNVVMTEDGRIIEVQGTAEGEPFTHEELLTLL
                     ALARGGIESIVATQKAALAN"
     gene            8671..9534
                     /locus_tag="OO357_002036"
     CDS             8671..9534
                     /locus_tag="OO357_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HCR5886991.1"
                     /translation="MIRSMTAYARREIKGEWGSATWEMRSVNQRYLETYFRLPEQFRS
                     LEPVVRERIRSRLTRGKVECTLRYEPDVSAQGELILNEKLAKQLVTAANWVKMKSDEG
                     EINPVDILRWPGVMAAQEQDLDAIAAEILAALDGTLDDFIVARETEGQALKALIEQRL
                     EGVTAEVVKVRAHMPEILQWQRERLVAKLEDAQVQLENNRLEQELVLLAQRIDVAEEL
                     DRLEAHVKETYNILKKKEAVGRRLDFMMQEFNRESNTLASKSINAEVTNSAIELKVLI
                     EQMREQIQNIE"
     gene            9755..10579
                     /gene="dinD"
                     /locus_tag="OO357_002037"
     CDS             9755..10579
                     /gene="dinD"
                     /locus_tag="OO357_002037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein D"
                     /protein_id="HCR5886992.1"
                     /translation="MNEHHQPFEEIKLINANGAEQWSARQLGKLLGYSEYRHFIPVLT
                     RAKEACENSGHTIDDHFEEILDMVKIGSNAKRALKDIVLSRYACYLVVQNGDPAKPVI
                     AAGQTYFTIQTRRQELADDEAFKQLREDEKRLFLRNELKEHNKQLVEAAQQAGVATAT
                     DFAIFQNHGYQGLYGGLDQKAIHQLKGLKKSQKILDHMGSTELAANLFRATQTEEKLK
                     RDSVNSKQQANTTHFDVGSKVRQTIQELGGTIPEELPTPQVSIKQLENSVKITEKK"
     gene            10870..11487
                     /locus_tag="OO357_002038"
     CDS             10870..11487
                     /locus_tag="OO357_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimeric intracellular cation channel family
                     protein"
                     /protein_id="HCR5886993.1"
                     /translation="MLLHILYLVGITAEAMTGALAAGRRRMDTFGVIIIATATAIGGG
                     SVRDILLGHYPLGWVKHPEYVIIVATAAVLTTIVAPVMPYLRKVFLVLDALGLVVFSI
                     IGAQVALDMGHGPIIAVVAAVTTGVFGGVLRDMFCKRIPLVFQKELYAGVSFASAVLY
                     IALQHYVSNHDVVIISTLVFGFFARLLALRLKLGLPVFYYSHEGH"
     gene            complement(11484..13166)
                     /gene="ligB"
                     /locus_tag="OO357_002039"
     CDS             complement(11484..13166)
                     /gene="ligB"
                     /locus_tag="OO357_002039"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigB"
                     /protein_id="HCR5886994.1"
                     /translation="MKVWMAILISILCWQSSVWAVCPAWSPARAQEEISRLQQQIKQW
                     DDDYWKEGKSEVEDGVYDQLSARLTQWQRCFVSEPRDVMMPPLNGAVMHPVAHTGVRK
                     MADKNALSLWMRERSDLWVQPKVDGVAVTLVYRDGKLNKAISRGNGLKGEDWTQKVSL
                     ISAVLQTVSGPLANSTLQGEIFLQREGHIQQQMGGINARAKVAGLMMRQGNSDTLNSL
                     AVFVWAWPDGPQLMTDRLKELATAGFTLTQRYTRAVKNADEVARVRNEWWKAKLPFVT
                     DGVVVRGAKEPESRHWLPGQAEWLVAWKYQPVAQVAEVKAIQFAVGKSGKISVVASLA
                     PVMLDDKKVQRVNIGSVRRWQEWDIAPGDQILVSLAGQGIPRIDDVVWRGAERTKPTP
                     PENRFNPLTCYFASDVCQEQFISRLVWLGSKQVLGLDGIGEAGWRALHQTHRFEHIFS
                     WLLLTPEQLQNTPGIAKSKSAQLWHQFNLARNQPFTRWVMAMGIPLTRAALNASDERS
                     WSQLLFSTEQFWQQLPGTGSGRARQVIEWKENAQIKKLGSWLAAQQITGFEP"
     gene            13424..14047
                     /gene="gmk"
                     /locus_tag="OO357_002040"
     CDS             13424..14047
                     /gene="gmk"
                     /locus_tag="OO357_002040"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000046969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HCR5886995.1"
                     /translation="MAQGTLYIVSAPSGAGKSSLIQALLKTQPLYDTQVSVSHTTRQP
                     RPGEVHGEHYFFVNHDEFKEMISRDAFLEHAEVFGNYYGTSREAIEQVLATGVDVFLD
                     IDWQGAQQIRQKMPHARSIFILPPSKIELDRRLRGRGQDSEEVIAKRMAQAVAEMSHY
                     AEYDYLIVNDDFDTALTDLKTIIRAERLRMSRQKQRHDALISKLLAD"
     gene            14102..14377
                     /gene="rpoZ"
                     /locus_tag="OO357_002041"
     CDS             14102..14377
                     /gene="rpoZ"
                     /locus_tag="OO357_002041"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013511051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HCR5886996.1"
                     /translation="MARVTVQDAVEKIGNRFDLVLVAARRARQMQVGGKDPLVPEEND
                     KTTVIALREIEEGLINNQILDVRERQEQQELEAAELQAVTAIAEGRR"
     gene            14396..16504
                     /gene="spoT"
                     /locus_tag="OO357_002042"
     CDS             14396..16504
                     /gene="spoT"
                     /locus_tag="OO357_002042"
                     /EC_number="3.1.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional GTP
                     diphosphokinase/guanosine-3',5'-bis pyrophosphate
                     3'-pyrophosphohydrolase"
                     /protein_id="HCR5886997.1"
                     /translation="MYLFESLNQLIQTYLPEDQIKRLRQAYLVARDAHEGQTRSSGEP
                     YITHPVAVACILAEMKLDYETLMAALLHDVIEDTPATYQDMEQLFGKSVAELVEGVSK
                     LDKLKFRDKKEAQAENFRKMIMAMVQDIRVILIKLADRTHNMRTLGSLRPDKRRRIAR
                     ETLEIYSPLAHRLGIHHIKTELEELGFEALYPNRYRVIKEVVKAARGNRKEMIQKILS
                     EIEGRLQEAGIPCRVSGREKHLYSIYCKMVLKEQRFHSIMDIYAFRVIVNDSDTCYRV
                     LGQMHSLYKPRPGRVKDYIAIPKANGYQSLHTSMIGPHGVPVEVQIRTEDMDQMAEMG
                     VAAHWAYKEHGETSTTAQIRAQRWMQSLLELQQSAGSSFEFIESVKSDLFPDEIYVFT
                     PEGRIVELPAGATPVDFAYAVHTDIGHACVGARVDRQPYPLSQPLTSGQTVEIITAPG
                     ARPNAAWLNFVVSSKARAKIRQLLKNLKRDDSVSLGRRLLNHALGGSRKLNEIPQENI
                     QRELDRMKLATLDDLLAEIGLGNAMSVVVAKNLQHGDASIPPATQSHGHLPIKGADGV
                     LITFAKCCRPIPGDPIIAHVSPGKGLVIHHESCRNIRGYQKEPEKFMAVEWDKETAQE
                     FITEIKVEMFNHQGALANLTAAINTTTSNIQSLNTEEKDGRVYSAFIRLTARDRVHLA
                     NIMRKIRVMPDVIKVTRNRN"
     gene            16511..17200
                     /gene="trmH"
                     /locus_tag="OO357_002043"
     CDS             16511..17200
                     /gene="trmH"
                     /locus_tag="OO357_002043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(18)-2'-O)-methyltransferase
                     TrmH"
                     /protein_id="HCR5886998.1"
                     /translation="MNPTRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVG
                     VHEVHAVWPGSRMRTMASAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNA
                     VDFREIDYTRPTCILMGQEKTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYE
                     AQRQRQNAGMYLRENSMLPEAEQQRLLFEGGYPVLAKVAKRKGLPYPHVNQQGEIEAD
                     ADWWATMQAAG"
     gene            17206..19287
                     /gene="recG"
                     /locus_tag="OO357_002044"
     CDS             17206..19287
                     /gene="recG"
                     /locus_tag="OO357_002044"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709432.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HCR5886999.1"
                     /translation="MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRY
                     EDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRMMTCQISDGSGILTMRFFNFSAA
                     MKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
                     ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQH
                     PAQRRLILEELLAHNLSMLALRAGAQRFHAQPLSANDTLKNKLLAALPFKPTGAQARV
                     VAEIEHDMALDVPMMRLVQGDVGSGKTLVAALAALRAIAHGKQVALMAPTELLAEQHA
                     NNFRNWFAPLGIEVGWLAGKQKGKARLSQQEAIASGQVQMIVGTHAIFQEQVQFNGLA
                     LVIIDEQHRFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDE
                     LPPGRTPVTTVAIPDTRRTDIIDRVRHACITEGRQAYWVCTLIEESELLEAQAAEATW
                     EELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVPNASLMI
                     IENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQ
                     KDLEIRGPGELLGTRQTGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIER
                     WMPETERYSNA"
     gene            complement(19321..20526)
                     /gene="gltS"
                     /locus_tag="OO357_002045"
     CDS             complement(19321..20526)
                     /gene="gltS"
                     /locus_tag="OO357_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/glutamate symporter"
                     /protein_id="HCR5887000.1"
                     /translation="MFHLDTLATLVAATLTLLLGRKLVHSVSFLKKYTIPEPVAGGLL
                     VALALLVLKKSMGWEVNFDMSLRDPLMLAFFATIGLNANIASLRAGGRVVGIFLIVVV
                     GLLVMQNAIGIGMASLLGLDPLMGLLAGSITLSGGHGTGAAWSKLFIERYGFTNATEV
                     AMACATFGLVLGGLIGGPVARYLVKHSTTPNGIPDDQEVPTAFEKPDVGRMITSLVLI
                     ETIALIAICLTVGKIVAQLLAGTAFELPTFVCVLFVGVILSNGLSIMGFYRVFERAVS
                     VLGNVSLSLFLAMALMGLKLWELASLALPMLAILVVQTIFMALYAIFVTWRMMGKNYD
                     AAVLAAGHCGFGLGATPTAIANMQAITERFGPSHMAFLVVPMVGAFFIDIVNALVIKL
                     YLMLPIFAG"
     gene            20806..22197
                     /gene="xanP"
                     /locus_tag="OO357_002046"
     CDS             20806..22197
                     /gene="xanP"
                     /locus_tag="OO357_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine/proton symporter XanP"
                     /protein_id="HCR5887001.1"
                     /translation="MSVSTLESENAQPVAQTQNSELIYRLEDRPPLPQTLFAACQHLL
                     AMFVAVITPALLICQALGLPAQDTQHIISMSLFASGVASIIQIKAWGPVGSGLLSIQG
                     TSFNFVAPLIMGGTALKTGGADVPTMMAALFGTLMLASCTEMVISRVLHLARRIITPL
                     VSGVVVMIIGLSLIQVGLTSIGGGYAAMSDNTFGAPKNLLLAGVVLALIILLNRQRNP
                     YLRVASLVIAMAAGYALAWFMGMLPESNEPMTQELIMVPTPLYYGLGIEWSLLLPLML
                     VFMITSLETIGDITATSDVSEQPVSGPLYMKRLKGGVLANGLNSFVSAVFNTFPNSCF
                     GQNNGVIQLTGVASRYVGFVVALMLIVLGLFPAVSGFVQHIPEPVLGGATLVMFGTIA
                     ASGVRIVSREPLNRRAILIIALSLAVGLGVSQQPLILQFAPEWLKNLLSSGIAAGGIT
                     AIVLNLIFPPEKQ"
     gene            22318..24027
                     /locus_tag="OO357_002047"
     CDS             22318..24027
                     /locus_tag="OO357_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5887002.1"
                     /translation="MKFIGKLLLYILIALLVVIAGLYFLLQTRWGAEHISAWVSENSD
                     YHLAFGAMDHRFSAPSHIVLENVTFGRDGQPAPLVAKSVDIALSSRQLTEPRHVDTIL
                     LENGTLNLTDQTAPLPFKADRLQLRDMAFNSPNSEWKLSAQRVNGGVVPWSPEAGKVL
                     GTKAQIQFSAGSLSLNDVPATNVLIEGSIDNDRVTLTNLGADIARGTLTGNAQRNADG
                     SWQVENLRMADIRLQSEKSLTDFFAPLRSVPSLQIGRLEVIDARLQGPDWAVTDLDLS
                     LRNMTFSKDDWQTQEGKLSMNASEFIYGSLHLFDPIINAEFSPQGVALRQFTSRWEGG
                     MVRTSGNWLRDGKTLILDDAAIAGLEYTLPKNWQQLWMETTPGWLNSLQLKRFSASRN
                     LIIDIDPDFPWQLTALDGYGANLTLVTDHKWGVWSGSANLNAAAATFNRVDVRRPSLA
                     LTANSSTVNISELSAFTEKGILEATASVSQTPQRQTHISLNGRGVPVNILQQWGWPEL
                     PLTGDGNIQLTASGDIQANAPLKPTVSGQLHAVNAAKQQVTQTMNAGVVSSGEVTSTE
                     PVR"
     gene            complement(24080..26398)
                     /gene="yicI"
                     /locus_tag="OO357_002048"
     CDS             complement(24080..26398)
                     /gene="yicI"
                     /locus_tag="OO357_002048"
                     /EC_number="3.2.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-xylosidase"
                     /protein_id="HCR5887003.1"
                     /translation="MKISDGNWLIQPGLNLIHPLQVFEVEQQDNEMVVYAAPRDVRER
                     TWQLDTPLFTLRFFSPQEGIVGVRIEHFQGALNNGPHYPLNILQDVKVTIENTERYAE
                     FKSGNLSARVSKGEFWSLDFLRNGERITGSQVKNNGYVQDTNNQRNYMFERLDLGVGE
                     TVYGLGERFTALVRNGQTVETWNRDGGTSTEQAYKNIPFYMTNRGYGVLVNHPQCVSF
                     EVGSEKVSKVQFSVESEYLEYFVIDGPTPKAVLDRYTRFTGRPALPPPWSFGLWLTTS
                     FTTNYDEATVNSFIDGMAERNLPLHVFHFDCFWMKAFQWCDFEWDPLTFPDPEGMIRR
                     LKAKGLKICVWINPYIGQKSPVFKELQEKGYLLKRPDGSLWQWDKWQPGLAIYDFTNP
                     DACKWYADKLKGLVAMGVDCFKTDFGERIPIDVQWFDGSDSQKMHNHYAYIYNELVWN
                     VLKDTVGEEEAVLFARSASVGAQKFPVHWGGDCYANYESMAESLRGGLSIGLSGFGFW
                     SHDIGGFENTAPAHVYKRWCAFGLLSSHSRLHGSKSYRVPWAYDDESCDVVRFFTQLK
                     CRMMPYLYREAARANARGTPMMRAMMMEFPDDPACDYLDRQYMLGDNVMVAPVFTEAG
                     DVQFYLPEGRWTHLWHNDELDGSRWHKQQHGFLSLPVYVRDNTLLALGNNDQRPDYVW
                     HEGTAFHLFNLQDGHEAVCEVPAADGSVIFTLKAARTGNTITVTGAGEAKNWTLCLRN
                     VVKVNGLQGGSQAESEQGLVVTPQGNALTITL"
     gene            complement(26408..27791)
                     /locus_tag="OO357_002049"
                     /pseudo
     CDS             complement(26408..27791)
                     /locus_tag="OO357_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418114.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide family
                     transporter"
     gene            28084..28178
                     /locus_tag="OO357_002050"
     tRNA            28084..28178
                     /locus_tag="OO357_002050"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28118..28120,aa:Sec,seq:tca)
     gene            28450..29661
                     /locus_tag="OO357_002051"
     CDS             28450..29661
                     /locus_tag="OO357_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5887004.1"
                     /translation="MNSSKAGGCHGINDIKVKTAKPKDKPYKLADGGGMYLLINTNGS
                     KYWRMKYRFAGKEKMLSIGVYPDVTLADAREKRSEARKLLAAGGDPGEAKKEEKIAQQ
                     MSLKNTFEAIAREWHQLKADRWSLRYRDEIIDTFEKDIFPYIGKRPIAEIKPMKLLEA
                     LRKMEKRGALEKMRKVRQRCGEVFRYAIVTGRADYNPAPDLASALATPKKVHFPFLTA
                     NELPHFLNDLAGYTGSIITKTATQIIMLTGVRTQELRFARWEDIDFETKLWEIPAEVM
                     KMKRPHIVPLSEQVIMLFKQLEPISKHHPLVFIGRNDPRKPISKESINQVIELLGYKG
                     RLTGHGFRHTMSTILHEQGFNSAWIEMQLAHVDKNSIRGTYNHALYLDGRREMMQWYA
                     DYIDSLSSRES"
     gene            30073..31116
                     /locus_tag="OO357_002052"
     CDS             30073..31116
                     /locus_tag="OO357_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HCR5887005.1"
                     /translation="MNDRLCFEVHDNKGYFVFPDTWFGPLLGEFEEVLDAYDADEISE
                     TSYINKLRRLAQREPDFIDIHAHLAYAFLEQNAPRKALNAALKGLAAGNRIIPESFCG
                     EIIWMHPENRPYLRALYAAILANVHLQRHQDAVMLTDKILAYNPEDNQGARWLLGSEL
                     LRTGDHERAFSVLKEHADEFSPYWYELGLLHFLNGEHVKAATAFRHGFATNTYIAEML
                     CGNLHPFPLAVWHDFSGSLDTAEDYYATYSPLWGQYPEALLFVNWLYNHSSVLHERSE
                     IIKCAEMLIQEDDFEICESILRQQEHLWKRIDKTLSEEIVQKCRNMNGEYIWPWILPF
                     SAAGMKHSSIQYQ"
     gene            31631..>31859
                     /locus_tag="OO357_002053"
     CDS             31631..>31859
                     /locus_tag="OO357_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887006.1"
                     /translation="MDENALRFASYWRNSLADAESGKGCFERKDAQNFTHWHGIAAGR
                     LDEAIVNKFFKGEKDDVETVNVILRPKVYFRL"
BASE COUNT         7988 a         8396 c         7905 g         7570 t
ORIGIN      
        1 gtcctccgta tcctgtcata cgcgtaaaac agccagcgct gacgtgattt agcaccgacg
       61 tagccccaat gttcgtccat ttcagcgcag acaatcacat cactgcccgg ttgtatgcgc
      121 gaggttaccg actgcggcct gagtttttta agtgacgtaa aaccgtgttg aggccaacgc
      181 ccataatgcg tgcactggcg cgacatccga cgccattcat ggccatatca atgattttct
      241 ggtgcgtacc gggctgagag gcggtgtaag tgaactgtag ttgccatgtt ttacggcaat
      301 gagagcagag atagcgctga tgtccggcag tgcttttgcc gttacgcacc acgccttcag
      361 tagcggagca ggaaggacat ctgatggaaa tggaagccac gcaagcacct taaaatcacc
      421 atcatacact aaatcagtaa gttggcagca ttacccaaaa acgggaaaag taatggtaaa
      481 gccacagcta aatacataga atccccagca catccataag tcagctattt actatgctcg
      541 aattgcttta caccgccctt ctctacctta ttcagccgct gatctggata cggctctggg
      601 tgcgcggacg taaggctccg gcctatcgaa aacgctgggg tgaacgttac ggtttttacc
      661 gccatccgct aaaaccaggc ggcattatgc tgcactccgt ctccgtcggt gaaactctgg
      721 cggcgatccc gttggtgcgc gcgctgcgtc atcgttatcc tgatttaccg attaccgtta
      781 caaccatgac gccaaccggt tcggagcgcg tacaatcggc tttcgggaag gatgttcagc
      841 acgtttatct gccgtatgac ctgcccgatg cgctcaaccg tttcctgaat aaagtcgacc
      901 ccaaactggt gttgattatg gaaaccgaac tatggcctaa cctgattgcg gcgctacata
      961 aacgtaaaat tccgctggtg atcgctaacg cgcgactctc tgcccgctcg gccgcaggtt
     1021 atgccaaact gggtaaattc gtccgccgct tgctgcgtcg tattacgctg attgctgcgc
     1081 aaaatgaaga agatggtgca cgttttgtgg cgctgggcgc aaaaaataac caggtaaccg
     1141 ttaccggtag cctgaaattc gatatttctg taactccgca gttggctgct aaagccgtga
     1201 cgctacgccg ccaatgggca ccacaccgcc cggtatggat tgccaccagc actcacgaag
     1261 gtgaagagag cgtggtcatt gccgcacatc aggcattgtt acagcaattc ccgaatttat
     1321 tgctcatcct ggtaccccgt catccagaac gcttcccgga tgcgattaac cttgtccgcc
     1381 aggctggact aagctatatc acacgctctt caggggaagt cccctccacc agcacgcagg
     1441 ttgtggttgg cgatacgatg ggcgagttga tgttactgta cggcattgcc gatctcgcct
     1501 ttgttggcgg ttcactggtt gaacgtggtg ggcataatcc gctggaagct gccgcacacg
     1561 ctattccggt attgatgggg ccgcatactt ttaactttaa agacatttgc gcgcggctgg
     1621 agcaggcaag cgggcttatt accgttaccg atgccactac gcttgcaaaa gaggtttcct
     1681 ctttactcac cgacgccgtt taccgtagtt tctatggccg tcatgccgtt gaagtactgt
     1741 atcaaaacca gggcgcgcta cagcgtctgc ttcaactgct ggaaccttac ctgccaccga
     1801 aaacgcattg aggttgttat gcaaaaacgg gcgatttatc cgggtacttt cgatcccatt
     1861 accaatggtc atatcgatat cgtgacgcgc gccacgcaga tgttcgatca cgttattctg
     1921 gcgattgccg ccagccccag taaaaaaccg atgtttaccc tggaagagcg tgtggcactg
     1981 gcacagcagg caaccgcgca tctggggaac gtggaagtgg tcgggtttag tgatttgatg
     2041 gcgaacttcg cccgtaatca acacgctacg gtgctgattc gtggcctgcg tgcggtggcg
     2101 gattttgaat atgaaatgca gctggcgcat atgaatcgcc acttaatgcc ggaactggaa
     2161 agtgtgtttc tgatgccgtc gaaagagtgg tcgtttatct cttcatcgtt ggtgaaagag
     2221 gtggcgcgcc atcagggcga tgtcacccat ttcctgccgg agaatgtcca tcaggcgctg
     2281 atggcaaaat tagcgtagcg tttatgccgg atggtatgcc atccggctcg catgaattac
     2341 ttctggcact gccgacaata aaacgttgcc cgctgcgcat gtttagtcgc cacaatcggc
     2401 gtaccgcaca cccgacacgg ctcacctttt cgcccgtaaa cctgcaattc ctgcgcgaaa
     2461 tagcccggtt taccatcact ttgcagaaaa tctttcagcg ttgtaccacc ctgctcaatc
     2521 gaacgcagca acaccgcttt aatcacccga gctaacaatt cacactctgc cagcgacagt
     2581 gatgacgcca gccgatccgg atggatcccc gccgcaaaca gtgattcgct ggcatagata
     2641 ttccctaccc ctaccaccag cttgttatcc atcagccacg gtttaatcgc cgtttttttc
     2701 ttcgcgcact tctgatgcag atactcacca ttgaaatcgt cgctaagcgg ctccggtcca
     2761 agatgggcca gcacattatg cccttccagc tctttggtcc acagccaggc accaaagcgg
     2821 cgcggatcgg tgtagcgcag cactttaccg ttgctcatca ccaaatccac atggtcatgc
     2881 ttttcagggg gaagttcttc tggaaggatg cgcaggctgc cagacatccc caaatgaatg
     2941 ataatccagc cctcaggcag ctccagcagc agatatttag cccgccgctg cacgctaagc
     3001 actggttgat cgcttaatcg gtagatctct tctgaaaccg gccagcgcaa gcgtccgttg
     3061 cgcaccaccg catgaagaat ggttgcacca acgagatgcg gttctatgcc gcgacggctg
     3121 gtttcaactt cgggtaattc aggcatagca tctccaggaa tgaacagatg caatcaatat
     3181 gggggcaagc agataacaaa aaaccccgcc ggagcgaggt ttttcgttac atcaaagcga
     3241 gaattatttg attttcgctt ctttgtagat cacgtgctga cgaacaactg gatcgaattt
     3301 tttcagttcc agtttttccg gcttagtacg tttgttcttc gtagtggtat agaagtgacc
     3361 agtaccagca gaagaaacca gcttgatttt ctcacgaata cctttagcca tgatttattt
     3421 cctctaagta cttagtactt ttcgccacgg gcacgcagtt cagccagaac tgtatcgatg
     3481 ccttttttat cgattacacg cataccttta gcagatacgc gcagggtgac aaaacgcttc
     3541 tcgctctcaa cccagaaacg gtgagagtgc aggttcggca ggaaacggcg tttagtcgcg
     3601 ttcagtgcgt gggaacggtt gttaccggtc accggacgct tgccagtaac ttggcagact
     3661 cgggacatgt ctattctcca aaaatcaaat tagctcgagc ttcgtatggg gtattggcgc
     3721 ctcgtcaggc tttacagccc ggtcatcgca gttctatgtg aactctcgat tgccaggccc
     3781 aaatgccaaa cccgagattc tcaaaggtgg cgtagtatac gctgactcag cgatgtgctc
     3841 aagtcccgaa cagacaaaga tcccgaagga tcgcgcatag cgggttaaat ccagccgcgt
     3901 tcggcaaaag aaacatactc tccacgccca atcacgatat ggtcgagcac gcgtaaatcc
     3961 atgaactgac aactctttat tatccgttca gtaatgagtt tatccgcttt actgggttca
     4021 gcacaacccg aagggtgatt atgtgcaagg atcagcgccg aggcgtttat ttttatcgct
     4081 tcgcggataa tttcccgagg atggacttca acatggttta gcgtgccgga aaaaagacgg
     4141 ctatgcgtta taacccggtg ttgtgagtcg agaaagatca ccataaagat ctcccgctcc
     4201 tcacccgtga gctggctttg taaaaattcc cgcgtcatct ccgggctgag taaagggctt
     4261 tcttcacgca tccgcacgtt gtagtaacgt cgcgccagtt cagcaatccc ctttaactgg
     4321 gcaaatttcg ctacgccaat tccatgaaca ccactaaatt gttcatattc agaggttaac
     4381 aagccataaa gagagccgaa attctccagc atctcttttg ccagggttaa tacatcttta
     4441 ccgcgcgttc cggtacgcag aaatagcgcc agcagctcga catccgttaa ggcgctaata
     4501 ccaaacttca gcattttttc gcgcggcatc aacagctgtg cattgttttt caccttcacc
     4561 tcctttgtgg tgctcgcatc ctgtcatagc cgttccgtta aatcgacggc cagttttcaa
     4621 tcctggaaag agcctcgcaa agtgaatctc aggtgaaggc acgacaacaa aagtattgtg
     4681 ataaaatcgc caacttctgg tgtcacccaa caggaaaaat catcatgagc ctggccggta
     4741 aaaaaatcgt tctcggcgtt agcggcggta ttgctgccta taaaacccct gaactggtgc
     4801 gtcgtttgcg cgatcgcggg gccgacgtcc gcgtagccat gaccgaagcg gcaaaagcct
     4861 ttatcacccc acttagcttg caggcggttt ctggttatcc cgtttccgac agtctgcttg
     4921 acccggcagc cgaagccgct atgggccata ttgagctggg taaatgggct gatttagtga
     4981 ttctcgcccc tgccacggca gatttgattg cccgtgttgc tgccggaatg gcgaatgacc
     5041 tggtatcgac gatttgtctg gctacacctg cgcctgtagc cgtgctcccc gccatgaacc
     5101 agcagatgta ccgtgccgct gccacgcagc ataatttaga ggtgcttgct tcccgtggtt
     5161 tgctcatctg ggggccagac agtggcagtc aggcttgtgg tgatatcggt cctggacgaa
     5221 tgctcgatcc gttaaccatt gtggatatgg cggtggcgca tttttcgccc gtcaacgacc
     5281 tgaaacatct gaacattatg attaccgccg gtccgacgcg tgaaccgctc gatccggtgc
     5341 gttatatctc taatcacagc tccggcaaga tgggttttgc tatcgccgcc gctgctgccc
     5401 gtcgtggcgc gaacgtcacg ctggtatcag gtccagtttc actaccgacg ccaccgtttg
     5461 ttaaacgtgt tgatgtgatg accgcgctgg aaatggaagc cgccgtgaat gcttctgtac
     5521 agcagcaaaa tatttttatc ggttgcgccg ccgtggcgga ttatcgcgca gctaccgtgg
     5581 ccccagaaaa aatcaaaaag caggccacgc agggtgatga attaacaata aaaatggtta
     5641 aaaaccccga tatcgtcgca ggcgttgccg cactaaaaga ccatcgaccc tacgtcgttg
     5701 gatttgccgc cgaaacaaat aatgtggaag aatacgcccg gcaaaaacgt atccgtaaaa
     5761 accttgatct gatctgcgcg aacgatgttt cccagccaac tcaaggattt aacagcgaca
     5821 acaacgcatt acaccttttc tggcaggacg gagataaagt cttaccgctt gagcgcaaag
     5881 agctccttgg ccaattatta ctcgacgaga tcgtgacccg ttatgatgaa aaaaatcgac
     5941 gttaagattc tggacccgcg cgttaggaag gaatttccgc tcccgactta tgccacctct
     6001 ggctctgccg gacttgacct gcgtgcctgt ctcgacgacg ccgtagaact ggctccgggt
     6061 gacactacgc tggttccgac cgggctggcg attcatattg ccgatccttc actggcggca
     6121 atgatgctgc cgcgctccgg attgggacat aagcacggta tcgtgcttgg taacctggta
     6181 ggattgatcg attccgacta tcagggccag ttgatgattt ccgtgtggaa ccgtggtcag
     6241 gacagcttca ccattcaacc tggcgaacgc atcgcccaga tgatttttgt tccggtagta
     6301 caggctgaat ttaatctggt ggaagatttc gacgccaccg accgcggtga aggcggcttt
     6361 ggtcactctg gtcgtcagta acacatacgc atccgaataa cgtcataaca tagccgcaaa
     6421 catttcgttt gcggtcatag cgtgggtgcc gcctggcaag tgcttatttt caggggtatt
     6481 ttgtaacatg gcagaaaaac aaactgcgaa aaggaaccgt cgcgaggaaa tacttcagtc
     6541 tctggcgctg atgctggaat ccagcgatgg aagccaacgt atcacgacgg caaaactggc
     6601 cgcctctgtc ggcgtttccg aagcggcact gtatcgccac ttccccagta agacccgcat
     6661 gttcgatagc ctgattgagt ttatcgaaga tagcctgatt actcgcatca acctgattct
     6721 gaaagatgag aaagacacca cagcgcgcct gcgtctgatt gtgttgctgc ttctcggttt
     6781 tggtgagcgt aatcctggcc tgacccgcat cctcactggt catgcgctaa tgtttgaaca
     6841 ggatcgcctg caagggcgca tcaaccagct gttcgagcgt attgaagcgc agctgcgcca
     6901 ggtattgcgt gaaaagagaa tgcgtgaggg tgaaggttac accaccgatg aaaccctgct
     6961 ggcaagccag atcctggcct tctgtgaagg tatgctgtca cgttttgtcc gcagcgaatt
     7021 taaataccgc ccgacggatg attttgacgc ccgctggccg ctaattgccg cgcagttgca
     7081 gtaatatgac gccggatgac ttttcatccg gcgagtttct ttaaacgcca aactcttcgc
     7141 gataggcctt aaccgccgcc agatgttccg ccatttccgg cttctcttcc agataggcaa
     7201 tcagatcttt cagggtgatg atagagatca ctttgcagtt gtaatcacgc tcaacttcct
     7261 gaatcgccga aatctcgccg cgcccgcgtt cctgacgatc gagcgaaatc aacacgccag
     7321 caagcgtcgc gccattggcc tgaataatct ccatcgactc gcgaatcgcc gttccggcgg
     7381 tgatcacatc atctaccagc attacgcgtc cttgtaacgc gctaccaacc agattgccgc
     7441 cttcaccgtg gtcttttgct tctttgcggt taaagcagta cggcaggtcc aggtcgtgat
     7501 gctccgccag tgccacagcg gttgtggtgg caatcgggat ccctttgtaa gcagggccaa
     7561 acagcagatc gaactcaatg ccggaatcca ccaacgcttc agcgtaaaaa cggcctaaca
     7621 gtgccagatc gcgcccggta ttaaacagcc cggcgttgaa gaaatagggg cttttgcgcc
     7681 cggatttcag cgtaaactcg ccaaacttta acacctgctt gctaagcgca aattcaataa
     7741 actggcgctg atatgatttc atgccttcgc tcctcatctt acttttctac agacaaaaaa
     7801 aaggcgactc atcagtcgcc ttaaaaatca gtttgccagc gccgccttct gcgtcgctac
     7861 aatggattcg attccccctc gggccagcgc caacaaggtg agtagctctt catgggtgaa
     7921 cggctcgcct tctgccgtcc cctgcacttc aatgatgcgc ccgtcttcgg tcatcactac
     7981 gttcatgtcg gtctctgcgg ctgagtcttc aacgtattcc agatcgcaaa ttgcttcacc
     8041 gtttacaata ccgacagaaa ctgcggctac catccctttc atcgggttgg ttttcagctt
     8101 gccgttttcc accagcttct gtagcgcatc tgccagcgcc acgcaggcac ccgtaatcga
     8161 cgcggtacga gtgccaccat cagcctgaag cacgtcgcag tccagcgtaa tggtgaactc
     8221 acccagcgct ttcaaatcta ctgccgcgcg aagagcacgg gcgatcagac gctggatttc
     8281 cattgtgcgt ccaccctgct tacctttcgc cgcttcacga gcgttacggg tgtgggtaga
     8341 acgtggcagc atgccgtact ctgcggtgat ccagccctgg ccctgacctt tcaggaagcg
     8401 cggcacgcct tcttcaatag aggcggtaca caatactttg gtatcgccaa attcgaccag
     8461 caccgagcct tctgcatgtt ttgtatagtt acgagtcagg gtaacgggac gcacctgatt
     8521 attgctacgg cctgctggac gcatattgaa atctccggct tgaaacaaat gtggctgcgc
     8581 attatacgga cttccggcgg ttattcctat cctgacaagg catcgatggc tataatcctt
     8641 ccacctctcc ttttataaac aggaacgtct atgatccgca gtatgaccgc ctacgcccgg
     8701 cgtgaaatca agggtgaatg ggggagcgca acctgggaaa tgcgctcggt aaaccagcgt
     8761 tatctggaaa cttactttcg tctgccggag cagttccgta gccttgaacc tgtcgttcgc
     8821 gagcgtattc gttctcgcct gacgcgcggt aaagtggaat gtaccctgcg ctatgagcca
     8881 gatgttagcg cacaaggtga gctgatcctc aatgaaaaac tggctaaaca gctggtaact
     8941 gccgcgaact gggtaaaaat gaagagtgac gaaggggaaa tcaacccggt tgatattcta
     9001 cgctggccgg gcgtgatggc agcccaggag caggatctcg acgcgattgc cgctgaaatt
     9061 ctcgcggcgt tggatggtac gctggacgac tttatcgtcg cgcgcgaaac cgaaggtcag
     9121 gcactgaaag cattgatcga gcagcgtctg gaaggcgtca ccgccgaagt ggtcaaagtc
     9181 cgcgcccata tgccggaaat tctgcaatgg cagcgtgagc gtctggtcgc gaagctggaa
     9241 gatgctcagg ttcagttgga aaacaaccgt ctggagcagg agttggttct gctggcacaa
     9301 cgaattgacg ttgccgaaga gctggaccgc ctcgaagcgc atgttaaaga gacgtacaac
     9361 attctgaaga aaaaagaagc ggttggtcgt cgtctggact ttatgatgca ggagttcaac
     9421 cgtgagtcga acactcttgc gtcgaaatct atcaatgccg aagtgacaaa ctccgccatc
     9481 gagctgaaag tgttgattga gcagatgcgc gagcagattc agaatattga gtaactcgta
     9541 acaccaattc tgacttttca gccttcacaa atgccgccca ctcaaacaga gcggcatttt
     9601 tcttccccgc aacttcaatt ctgttttgcg tgcctgctcc agattttgcg atgttttttt
     9661 gcccagcaca ctgagaacgt gagatactca caactgtata taaatacagt tacagattta
     9721 ctttctttgc aatcgatatc acatggagtg ggcaatgaac gaacatcatc aaccttttga
     9781 agagataaaa ctgattaatg caaacggagc agaacaatgg tcagcaagac aacttgggaa
     9841 actactgggt tattcagagt atcgtcactt tatacctgta ttaacgcgcg ccaaagaggc
     9901 ctgtgagaac agtggtcaca caattgatga ccatttcgag gagatcctcg atatggtcaa
     9961 aattggctca aatgccaaaa gagcattaaa agacatcgta ctctcccgct atgcctgtta
    10021 cctggtagta caaaacggcg accccgcgaa accggtcatt gcggcagggc agacttattt
    10081 tactatccag acccgacggc aggagcttgc tgacgatgaa gcatttaagc aacttcgtga
    10141 agacgaaaag cgtctgtttc tgagaaatga gttgaaagaa cataacaaac aattggttga
    10201 ggcagcacag caagctgggg tagcgacagc tactgatttc gccatatttc agaatcatgg
    10261 ttaccagggg ctgtatggtg gattagatca gaaagccatc caccaactga aggggctgaa
    10321 aaagagtcag aagatcctgg atcatatggg ttcaacagaa ctggcggcta atctctttcg
    10381 agctacccaa acagaagaaa aactcaagcg ggatagcgtg aattcaaaac agcaagctaa
    10441 taccacacac tttgacgtgg gtagcaaggt gaggcaaacc attcaggaac ttggcggaac
    10501 catacctgag gagttgccga ccccgcaggt cagcatcaag cagttggaaa acagtgtaaa
    10561 aattacagag aagaaatagc agaaacgaaa aactcttttt gttgattgag acacccgatg
    10621 cgtaagccaa ggtccaggtg cagtgaacac aatggctaaa cattgcacct ttcttttccc
    10681 ccctcagttt taacctattt tttcttatgc attttctcag acaagaagtc agaagaatgc
    10741 atctacgcta cagaaaatag cgatttcaca taactaaaag ttatcaattt cctctcacct
    10801 taaaaaatat caatcgtgac aatgcgcaca aatcgctacc ctgccagaca gatttttagg
    10861 gagagaacca tgctgttaca cattttgtat ttggttggca ttactgccga agccatgacg
    10921 ggggcgctgg cggccgggcg acggcgcatg gatacatttg gcgtaattat tattgcaacc
    10981 gccaccgcaa ttggcggagg gtcagtgcgc gatattctgc tgggccacta tccgctcggc
    11041 tgggtcaaac acccggaata tgtgattatc gtcgctaccg ccgcagtact caccactatc
    11101 gttgctcctg taatgcctta cctgcgcaaa gtgtttctgg tgctcgatgc gctcggactg
    11161 gtggtctttt ctatcatcgg cgcacaggtt gcactggata tggggcacgg cccaattatt
    11221 gccgttgtcg cggcggtaac caccggtgta tttggcggcg ttttgcgcga tatgttctgt
    11281 aaacgcattc cgctggtctt tcaaaaagag ttgtatgccg gtgtctcatt tgcctccgcc
    11341 gtgctgtaca tcgcgctgca acactatgtt tctaaccatg atgtggtgat tatctccacc
    11401 ctggtattcg gcttttttgc tcgtttactg gcgctacgtc ttaagctggg attaccggtc
    11461 ttttactaca gccacgaagg tcactaaggt tcaaaacctg tgatctgctg ggcagccagc
    11521 caactgccca gcttcttgat ttgcgcattt tccttccatt caataacctg tctggcgcgc
    11581 ccagatccag tccccggcag ctgctgccag aactgctccg tgctaaataa aagttgcgac
    11641 caggaccgtt catcactggc attaagcgcc gcccgggtta gcggtattcc cattgccatc
    11701 acccagcgag taaaaggctg gttacgagcc agattaaact gatgccataa ctgcgcactt
    11761 ttacttttcg cgatccccgg cgtgttctgt aattgctctg gcgttaataa aagccaggaa
    11821 aagatatgct caaagcgatg agtctgatgc agcgcgcgcc aaccggcctc accaatgcca
    11881 tccagcccca gaacctgttt tgaccccagc cagactaagc gtgaaatgaa ctgttcctga
    11941 caaacatcag aagcaaagta gcaggtcaac gggttaaagc ggttttctgg tggtgtcggt
    12001 tttgtacgtt ctgcaccgcg ccagaccaca tcatcaatgc gaggaatacc ctgaccggca
    12061 aggctgacga ggatctgatc accaggcgca atatcccact cctgccagcg cctgacggaa
    12121 ccaatattca cccgctggac ttttttatca tccagcatga caggtgcgag tgacgcaacc
    12181 accgatattt taccgctctt acccaccgca aactgaatcg ccttcacttc ggcaacctga
    12241 gctacaggtt gatatttcca ggccaccagc cactccgcct ggcccggtag ccaatgacgg
    12301 gattcgggtt ccttagcccc tcgcacaacc acgccatcgg tgacgaaggg taatttcgct
    12361 ttccaccact cattgcgtac gcgcgcaacc tcatcagcat ttttaaccgc acgggtatac
    12421 ctctgcgtta gagtaaaacc tgcggtagcc agctccttta aacgatcggt cattaactgc
    12481 ggtccatccg gccatgccca gacaaaaaca gccagtgagt ttagcgtgtc gctattcccc
    12541 tggcgcatca tcaggccagc aacttttgcg cgggcattta ttccccccat ttgttgttgg
    12601 atatgcccct cgcgctggag aaatatttcc ccctgaagcg tactgttggc taaaggcccg
    12661 ctaacggttt gcagcacagc ggaaattaag ctaactttct gcgtccagtc ctcgcctttc
    12721 aggccattac cgcgactgat tgctttgttc agtttcccgt cccgataaac cagggttacc
    12781 gcaacgccat caacttttgg ctgcacccaa agatcgctac gttctcgcat ccacagactt
    12841 aatgcgtttt tatccgccat tttgcgtaca cccgtatggg caaccggatg cataaccgca
    12901 ccatttaaag gcggcatcat cacgtcgcgg ggctcgctca caaagcagcg ttgccactgc
    12961 gtaagacggg cgcttaactg atcgtaaaca ccgtcttcca cctcactttt tccttccttc
    13021 cagtagtcat cgtcccactg ttttatttgc tgttgcaggc gggaaatttc ttcctgtgct
    13081 ctggctggcg accaggccgg acagaccgcc cacacagatg attgccagca caagatactt
    13141 attaatatcg ccatccatac tttcatcttc acctccgctg tagtcaggct ggcagatata
    13201 caacgtggtg aaggcaaagc cgagtggcaa aaacggagtc tgcgaggacg cttcctgaga
    13261 atcgtcttta ttgcagtaaa tcacaggcaa atgcggaaac agctacgcaa aacgcaacaa
    13321 ctttgcgcaa aaagtgtgag caagggctac gtcacatggc cgcgccgtgt ataataagct
    13381 cgtatgtagg ctttatttcg ctaatcacat acgaaagata ctcatggctc aaggcacgct
    13441 ttatattgtt tctgccccca gtggcgcggg taaatccagc ctgattcagg ctttattaaa
    13501 aacccaaccg ttgtatgaca cccaggtttc tgtttcacac accacacgcc aaccgcgtcc
    13561 tggtgaagtc cacggtgaac attatttctt tgttaatcat gatgaattta aagaaatgat
    13621 tagcagagat gcgttcctcg aacacgcaga agtttttggt aattactatg gcacttcgcg
    13681 tgaggccatt gagcaagtac tggcgaccgg tgtcgatgtt tttctcgata tcgactggca
    13741 gggcgcgcag caaattcgcc agaagatgcc gcacgcgcgg agtatcttta ttttaccgcc
    13801 gtccaaaatt gaactggacc gccgtctacg cggtcgcggt caggacagcg aagaggtcat
    13861 tgcaaagcgt atggcgcaag ctgttgcaga aatgagccat tacgccgaat atgattatct
    13921 gattgtgaat gatgacttcg ataccgcgtt gaccgatttg aagaccatta ttcgcgccga
    13981 acgtctgcgc atgagccgcc aaaagcagcg tcatgacgct ttaatcagca aattgttggc
    14041 agactgaacc tgatttcagt atcatgccca gtcatttctt cacctgtgga gctttttaag
    14101 tatggcacgc gtaactgttc aggacgctgt agagaaaatt ggtaaccgtt ttgacctggt
    14161 actggtcgcc gcgcgtcgcg ctcgtcagat gcaggtaggc ggaaaggatc cgctggtacc
    14221 ggaagaaaac gataaaacca ctgtaatcgc gctgcgcgaa atcgaagaag gtctgatcaa
    14281 caaccagatc ctcgacgttc gcgaacgcca ggaacagcaa gagctggaag ccgctgaatt
    14341 acaagccgtt accgctattg ctgaaggtcg tcgttaatca caaagcgggt cgcccttgta
    14401 tctgtttgaa agcctgaatc aactgattca aacctacctg ccggaagacc aaatcaagcg
    14461 tctgcggcag gcgtatctcg ttgcacgtga tgctcacgag gggcaaacac gttcaagcgg
    14521 tgaaccctat atcacgcacc cggtagcggt tgcctgcatt ctggccgaga tgaaactcga
    14581 ctatgaaacg ctgatggcgg cgctgctgca tgacgtgatt gaagatactc ccgccaccta
    14641 ccaggatatg gaacagcttt ttggtaaaag cgtcgccgag ctggtagagg gggtgtcgaa
    14701 acttgataaa ctcaagttcc gcgataagaa agaggcgcag gccgaaaact ttcgcaagat
    14761 gattatggcg atggtgcagg atatccgcgt catcctcatc aaacttgccg accgtaccca
    14821 caacatgcgc acgctgggct cacttcgccc ggacaaacgt cgccgcatcg cccgtgaaac
    14881 tctcgaaatt tacagcccgc tggcgcaccg tttaggtatc caccacatta aaaccgaact
    14941 cgaagagctg ggttttgagg cgctgtatcc caaccgttat cgcgtaatca aagaagtggt
    15001 gaaagccgcg cgcggcaacc gtaaagagat gatccaaaaa atcctctcgg aaatcgaagg
    15061 gcgtttgcag gaagcgggaa ttccctgccg cgtcagtggt cgcgagaagc atctttattc
    15121 gatttactgc aaaatggtgc tcaaagagca gcgttttcac tcaatcatgg acatctacgc
    15181 tttccgcgtg atcgtcaatg attccgacac ctgttatcgc gtgctcgggc aaatgcatag
    15241 cctgtacaag ccgcgtccgg gtcgcgtgaa agactacatc gccattccaa aagcgaacgg
    15301 ctatcagtct ttgcacacct cgatgatcgg cccgcacggt gtgccggttg aggtccagat
    15361 ccgtaccgaa gatatggatc agatggcgga gatgggtgtt gccgcgcact gggcttataa
    15421 agagcacggc gaaaccagca ccaccgcgca aatccgcgcc cagcgctgga tgcaaagcct
    15481 gctggagctg caacagagcg ccggtagttc gtttgaattt atcgagagcg ttaaatccga
    15541 tctcttcccg gatgagattt acgttttcac accggaaggg cgaattgtcg agctgcctgc
    15601 cggtgcaacg cccgtcgact tcgcttatgc agtgcatacc gatatcggtc atgcctgcgt
    15661 gggcgcacgc gttgaccgcc agccttaccc gctgtcgcag ccgcttacca gcggtcaaac
    15721 cgttgaaatc attaccgcac cgggcgctcg cccgaatgcc gcgtggctga actttgtcgt
    15781 tagctcaaaa gcgcgcgcca aaattcgtca gttgctgaaa aacctcaagc gtgatgattc
    15841 tgtaagcctg ggccgtcgtc tgctcaacca tgctttgggt ggtagccgta agctgaatga
    15901 aatcccgcag gaaaatattc agcgcgagct ggatcgcatg aagctggcaa cgcttgacga
    15961 tctgctggca gaaatcggac ttggtaacgc aatgagcgtg gtggtcgcga aaaatctgca
    16021 acatggggac gcctccattc caccggcaac ccaaagccac ggacatctgc ccattaaagg
    16081 tgccgatggc gtactgatca cctttgcgaa atgctgccgc cctattcctg gcgacccgat
    16141 tatcgcccac gtcagccccg gtaaaggtct ggtgatccac catgaatcct gccgtaatat
    16201 ccgtggctac cagaaagagc cagagaagtt tatggctgtg gaatgggata aagagacggc
    16261 gcaggagttc atcaccgaaa tcaaggtgga gatgttcaat catcagggcg cgctggcaaa
    16321 cctgacggcg gcaattaaca ccacgacttc gaatattcaa agtttgaata cggaagagaa
    16381 agatggtcgc gtctacagcg cctttattcg tctgaccgct cgtgaccgtg tgcatctggc
    16441 gaatatcatg cgcaaaatcc gcgtgatgcc agacgtgatt aaagtcaccc gaaaccgaaa
    16501 ttaatgtttt atgaacccaa cacgttatgc acgcatctgc gaaatgctcg ccaggcggca
    16561 gcctgatctg accgtctgca tggagcaggt ccacaaacct cataacgttt ctgcgattat
    16621 tcgtaccgca gatgccgttg gcgtacatga agttcacgcc gtctggcctg gtagccgcat
    16681 gcgcaccatg gcttcggcag cggcgggtag taacagctgg gtacaggtga aaacacaccg
    16741 caccattggc gatgccgtcg ctcatctcaa aggccagggc atgcagattc tggcaaccca
    16801 tctttctgat aatgctgtcg atttccgcga aattgattac actcgcccga cctgcatttt
    16861 gatgggacag gagaaaacgg gcatcacgca ggaagcattg gccctggcgg atcaggacat
    16921 catcattccg atgatcggca tggtgcagtc gctgaatgtt tccgttgcct cagccctcat
    16981 tctttacgaa gcccagcgtc agcggcaaaa tgcaggcatg tacctgcgtg aaaacagcat
    17041 gttgccggaa gcagagcaac aacgcctgtt gtttgaaggc ggctatccgg tgctggcgaa
    17101 agtcgcaaaa cgcaaaggcc tgccttatcc ccacgtcaat cagcaaggcg agatcgaagc
    17161 tgatgccgac tggtgggcta ctatgcaggc tgcagggtaa gtgccatgaa aggtcgcctg
    17221 ttagatgctg tcccactcag ttccctaacg ggcgttggcg cagcacttag taacaaactg
    17281 gcgaaaatca acctgcatac cgtgcaggat ctactcttac accttcccct gcgctacgaa
    17341 gatcgcaccc atctctaccc catcggagaa ctactgccgg gcgtttatgc cacggtggaa
    17401 ggcgaagtgc tgaactgcaa tatctccttc ggcggtcggc ggatgatgac ctgccagatc
    17461 agcgacggtt ccggcatcct caccatgcgc tttttcaatt tcagcgcggc aatgaaaaat
    17521 agcctggcga cgggccgccg tgtactggct tatggcgaag caaagcgcgg taaatatggt
    17581 gcggagatga tccacccgga ataccgcgtg cagggcgatc tcagcacgcc agaattacag
    17641 gaaacgctca cgccggttta tccaacaacg gaaggcgtaa agcaggccac gctgcgtaaa
    17701 ttaaccgacc aggcgctgga tctgctcgac acctgcgcca ttgaagaact cctgccgccg
    17761 gaactgtcac aaggaatgat gacgctaccg gaagcgttgc gcactttgca ccgcccgcca
    17821 ccgacgctac agcttagcga tctggaaacc gggcagcatc cggcgcaacg tcgtctgatt
    17881 ctggaagaac tgctggcgca caacctcagc atgttagcct tacgtgccgg agcacagcgt
    17941 tttcatgccc agccgctgag cgccaatgac acgctgaaaa ataaactcct cgccgcctta
    18001 ccgttcaagc caacgggcgc acaggcacgc gtagtggcgg agatcgagca tgatatggcg
    18061 ctggatgtgc cgatgatgcg cctggtacag ggcgatgtag gttccggtaa aacgctggtc
    18121 gccgccctcg ccgcgctgcg tgcgattgcc cacggcaaac aggtggcact gatggcacca
    18181 accgaattac tcgccgagca gcacgccaat aacttccgca actggtttgc accgctcggt
    18241 atcgaagtgg gctggctcgc cggtaagcag aaaggcaaag cgcgactgtc tcagcaggaa
    18301 gccatcgcca gcggtcaggt gcagatgatt gtcggtacac acgccatctt ccaggaacag
    18361 gtgcagttta acggcctggc gctggtgatt atcgacgaac agcatcgttt tggcgtgcat
    18421 cagcgtctgg cattgtggga gaaaggccag cagcagggct tccatccgca tcagttgatc
    18481 atgaccgcca cgccgatccc ccgcacgctg gcaatgacgg cgtatgccga tctcgatacc
    18541 tcggtgatag atgagctgcc gccaggccgc acgccagtga ctacggtcgc tattcctgat
    18601 acccgccgta ccgacatcat tgaccgcgtg cgccacgcct gcataactga aggtcgtcag
    18661 gcgtactggg tttgtacgtt gattgaagag tcagagttac tggaagcgca ggcggcggaa
    18721 gctacctggg aagagttgaa actggcgcta ccagaattga atgttggcct ggtacacggg
    18781 cggatgaaac cagccgagaa acaggcggtg atggcgtcgt ttaaacaagg tgagctacat
    18841 ctgctggttg ccactaccgt tattgaagtc ggtgttgatg tgcctaacgc cagcctgatg
    18901 attatcgaaa accctgagcg tctgggtctg gcgcagttac accagctgcg cgggcgcgta
    18961 ggtcgtggcg cggtggcttc tcactgcgtg ctgctctaca aaacgccgct ttctaaaacg
    19021 gcgcaaattc gcctgcaagt gctgcgcgac agtaacgacg gttttgtgat tgcgcaaaaa
    19081 gatctggaga ttcgcggccc tggcgaattg ttaggcacgc gtcagacggg taatgctgaa
    19141 tttaaagtgg cggatttact gcgcgatcag gcgatgatcc cggaagttca gcgcctggcg
    19201 cgacatattc acgaacgtta cccacaacag gcaaaagcct tgatagaacg ctggatgccg
    19261 gagacagaac gttactcgaa tgcgtaaaag gcggcggtga gaagaccgcc gcttcatcgg
    19321 ttaaccggca aaaatcggca acattaaata cagcttaatc accagcgcat tgacgatatc
    19381 gataaagaac gcaccgacca tcggcaccac caaaaacgcc atgtgcgacg ggccaaagcg
    19441 ttcagtgatc gcctgcatgt tggcgattgc cgttggcgtt gcaccgaggc caaaaccaca
    19501 gtgacccgca gccagcactg ccgcatcgta gtttttgccc atcatgcgcc aggtaacgaa
    19561 gatggcatac aacgccatga agatggtctg taccaccaga atcgccagca tcggcagcgc
    19621 cagcgaagcc agctcccaca gtttcagccc catcaacgcc atcgccagga acaacgacaa
    19681 gcttacgtta cccagcacgg ataccgcacg ctcaaagacg cggtaaaagc ccattattga
    19741 cagaccgttg ctcagaatca cgccaacaaa cagtacacag acgaaggtcg gcagttcaaa
    19801 agcagtgcca gccaaaagtt gcgcaacaat tttccccacc gtcaggcaga tagcaatcag
    19861 cgcgatagtt tcaatcagca ccaacgaggt gatcatgcgt cccacatccg gcttttcaaa
    19921 cgccgtcggg acttcctggt catccggaat accgttcggc gtggtggagt gtttcaccag
    19981 atagcgcgcc accggaccgc caatcaagcc gcccagcacc agaccgaacg ttgcacaggc
    20041 catcgccact tccgtcgcat tggtgaagcc ataacgttca atgaacaatt tactccacgc
    20101 agcgcccgta ccgtgaccgc cggaaagcgt aatagaaccg gccaacagcc ccatcagcgg
    20161 atcaagccct aacaggctag ccataccaat gccaatggca ttttgcatca ccaacagacc
    20221 aacaaccaca atcaagaaga tgccaaccac acgcccaccg gcacgcaaac tggcaatgtt
    20281 ggcgttcagg ccaatggtgg cgaagaaagc cagcattaac ggatcgcgca gggacatatc
    20341 aaagttgact tcccagccca tgcttttttt cagtactagt agcgccagcg ccaccaacaa
    20401 accacccgca acaggttccg gtatggtgta tttcttcaaa aaggagacgg aatggaccaa
    20461 cttacgcccg agcagcaacg tcagcgttgc ggcaacaagc gttgctaaag tatcgagatg
    20521 aaacatagtt actcctttgt atccgcatca ctctttcata ctcgtcatac ttcatcgcgc
    20581 agtggcaact tcatgaacta tgacgggtat cgagttatgc tttcctgggt caaattctca
    20641 gctgttaatg aacaaatggc atggatttta agcagaaaaa taccaaaagt tatataaaaa
    20701 agtgcattta tctcatttaa atatttagat gaggatgtgg caaacgtttg cttttctctt
    20761 tcggtcagct aaaatgcccg ctttgctacc acgggattgt tttcgatgtc tgtttccacc
    20821 ctcgagtcag aaaatgcgca accggttgct cagactcaaa acagcgaact gatttaccgt
    20881 cttgaagatc gtccgccgct tcctcaaacc ctgtttgccg cctgtcagca tctgctggcg
    20941 atgttcgttg cggtgatcac gccagcgcta ttaatctgcc aggcgctggg tttaccggca
    21001 caagacacgc aacacattat tagtatgtcg ctgtttgcct ccggtgtggc atcgattatt
    21061 caaattaagg cctggggtcc ggttggctcc gggctgttgt ctattcaggg caccagcttc
    21121 aactttgttg ccccgctgat tatgggcggt accgcgctga aaaccggtgg tgctgatgtt
    21181 cctaccatga tggcggcttt gttcggcacg ttgatgctgg caagttgcac cgagatggtg
    21241 atctcccgcg ttctgcatct ggcgcgccgc attattacgc cgctggtttc tggcgttgtg
    21301 gtgatgatta tcggcctgtc gctaattcag gtggggctga cctccattgg cggcggttac
    21361 gcagccatga gcgataacac cttcggcgca ccgaaaaatc tgctgctggc aggcgtggtt
    21421 ttagccttaa ttatcctgct taaccgtcaa cgtaaccctt acttacgcgt ggcctcactg
    21481 gtaattgcga tggcggccgg atatgcgctg gcgtggttta tgggcatgtt gccagaaagc
    21541 aacgaaccga tgacgcaaga actgattatg gtgccaacgc cgctctatta cggtcttggc
    21601 attgaatgga gtctgctgct gccgctgatg ctggtcttta tgatcacttc gctggaaacc
    21661 attggcgata tcacggcgac ctctgacgtt tccgaacagc cagtgtccgg tccgctgtac
    21721 atgaaacgcc tgaaaggcgg cgtgctggca aacggcctga actcgtttgt ttcggcggtg
    21781 tttaacacct tcccgaactc ctgcttcggg caaaacaacg gagtgatcca gttgactggt
    21841 gttgccagcc gctatgtcgg ttttgtcgtc gcgctgatgt tgatcgtgct gggtctgttc
    21901 ccggcagtga gcggttttgt acaacacatt ccagaaccgg ttctgggcgg cgcaacgctt
    21961 gtaatgtttg gcaccatcgc cgcctccggt gtgcgtatcg tttctcgtga gccgctgaac
    22021 cgtcgggcga ttctgattat cgcgctgtcg ctggcggttg gtctgggcgt gtctcagcag
    22081 ccgctgattt tgcagtttgc ccctgaatgg ctgaaaaacc tgctctcctc cgggatcgcc
    22141 gcgggcggta ttactgccat cgtgctgaat ctgattttcc caccagaaaa acagtaattc
    22201 actggcggcg gtaagataat taccgccgca gtctcacctc tttcaccatt cccgccttga
    22261 ggattgcgcg taaatcgtgc ataactccct tatgtgcatt tcacgggatg gaagaccatg
    22321 aaatttattg ggaagctgct tctctacatt ctcatcgctc tgttagtggt gatcgctggc
    22381 ctctattttc ttctgcaaac ccgctgggga gcagaacata tcagcgcatg ggtttccgag
    22441 aatagcgact atcatctggc cttcggggcg atggatcacc gtttttccgc gccatctcat
    22501 atcgtgctgg agaatgtcac gtttggtcgt gatggtcagc ccgcgcccct ggtggcaaaa
    22561 agtgtcgaca ttgcgctaag cagtcgccaa ctgaccgaac cacgccatgt cgataccatc
    22621 ctgctggaaa acgggacgct gaatctcacc gaccagaccg cgccgctacc gttcaaagcc
    22681 gatcgtctgc aactgcgtga tatggcgttt aatagcccga atagcgaatg gaaactgagc
    22741 gcgcagcggg taaatggcgg cgtggttccg tggtcaccag aagccggtaa agtgctgggt
    22801 acgaaggcgc agattcagtt tagtgccgga tcgctttcgc tcaatgatgt tcctgccacc
    22861 aatgtactga ttgaaggcag tattgataac gatcgcgtta cgctgactaa cctgggtgcc
    22921 gacatcgccc gcgggacatt aaccggaaac gcgcagcgta acgccgacgg cagctggcaa
    22981 gtggaaaatc tgcgcatggc ggatatccgt ctacaaagcg aaaaatcgct aaccgacttc
    23041 tttgcgccat tacgctctgt cccgtcgttg cagattggtc gcttggaagt catcgatgct
    23101 cgtctgcaag gtccggactg ggcggtgacc gacctcgatc tcagcttgcg caacatgacc
    23161 ttcagtaaag atgactggca gacacaggaa ggcaaactgt cgatgaacgc cagcgagttc
    23221 atttatggtt cgctgcattt atttgacccg attataaacg cggaattttc cccgcagggc
    23281 gtagcgctgc gccagttcac cagccgctgg gaagggggta tggtcagaac gtcagggaac
    23341 tggctgcgtg acggtaaaac gttgatcctt gatgatgcgg caattgccgg gctggaatat
    23401 accttgccga aaaactggca acagttgtgg atggaaacga cacccggttg gttaaacagc
    23461 ctgcaactga agagatttag cgccagccgc aatctgatca ttgatatcga ccctgacttc
    23521 ccgtggcagc tcaccgcgct cgatggttac ggtgccaacc tgacgctggt taccgatcat
    23581 aaatggggcg tctggagtgg ctcggcgaat ctgaatgccg ccgccgcgac attcaatcgt
    23641 gttgatgttc gtcgcccgtc gctggcgctg accgccaaca gcagcacggt gaatatcagc
    23701 gaactgagtg catttactga aaaaggcatt ctggaagcca ccgcaagtgt ttcacaaacg
    23761 ccacaacgtc agacccatat cagcctgaat ggacgcggtg tgccggtgaa tattttgcaa
    23821 caatggggat ggcctgaatt accgttgact ggcgacggca atattcagct taccgccagt
    23881 ggagatattc aggccaatgc cccgctgaaa cctacggtta gcgggcaatt gcatgccgtg
    23941 aacgccgcaa agcagcaagt gactcaaacc atgaatgcgg gcgtcgtttc cagcggtgaa
    24001 gttacatcga cggagccggt gcggtaaaca tcaatctcgc cgggtacgac tcagacgtac
    24061 ccggcattcc gtcaatagat tacaacgtaa ttgtcagcgc attcccttga ggcgtcacca
    24121 ccagcccctg ctcactttca gcctgcgaac cgccttgcag accatttact ttcacaacat
    24181 tgcgcaagca cagcgtccag ttcttcgcct cgcccgcacc agtcacagta atcgtgttgc
    24241 cagtacgtgc tgcttttaaa gtaaagatca ccgatccgtc agcagcaggc acttcacaga
    24301 cggcttcatg cccgtcttgc agattaaaga ggtggaatgc cgtgccttcg tgccacacgt
    24361 aatcgggacg ttgatcgttg ttgcccagcg ccagtagagt gttatcacgc acataaacgg
    24421 gcagactcag gaagccgtgc tgctgtttat gccagcgact accgtcgagt tcatcgttgt
    24481 gccacaggtg tgtccagcga ccttccggca ggtagaactg cacatcgccc gcttcagtga
    24541 acaccggcgc aaccatcacg ttgtcgccta acatgtattg acggtcaagg taatcacaag
    24601 ccggatcgtc cgggaactcc atcatcatgg cccgcatcat cggcgtaccc cgcgcgttcg
    24661 cacgcgcagc ttcacgatac agatacggca tcatgcggca tttcagttgc gtgaagaagc
    24721 gcaccacatc acaggactca tcatcgtagg cccacggcac acgataagat ttgctaccgt
    24781 gtaaacggct atggctggag agcaaaccaa acgcgcacca gcgtttgtaa acgtgcgccg
    24841 gagcggtatt ttcaaagccg ccgatatcgt ggctccagaa gccaaaacct gaaaggccaa
    24901 tagacaaacc accgcgcagg ctttccgcca ttgattcgta gttagcgtaa caatcgccac
    24961 cccagtgtac cgggaatttc tgcgcaccga cggaggccga gcgggcaaac aagacagctt
    25021 cttcctcacc aacggtgtcc ttgagcacgt tccacaccag ttcgttgtag atgtacgcat
    25081 aatggttatg cattttctgc gaatcggaac cgtcaaacca ctgaacatca attgggatac
    25141 gttcgccaaa gtcggtctta aagcaatcaa cgcccatcgc gaccagacct ttcagtttgt
    25201 cggcgtacca tttgcaggca tccggattgg taaagtcata aatcgccaga cctggctgcc
    25261 atttatccca ctgccatagc gaaccgtccg ggcgtttgag taaatagcct ttctcttgta
    25321 actctttaaa gacgggggat ttttgaccga tataggggtt aatccagacg cagattttca
    25381 gccctttcgc tttcaggcgg cggatcatcc cttccgggtc agggaaagtc agcgggtccc
    25441 actcaaaatc gcaccactgg aaggctttca tccagaaaca gtcaaagtgg aaaacatgca
    25501 gcggcagatt gcgttccgcc ataccatcga taaagctgtt taccgtcgct tcgtcgtagt
    25561 tggtggtaaa tgaagtggtt agccacaggc cgaaggacca cgggggcggc agcgccggac
    25621 gaccagtaaa gcgggtataa cgatcaagta ccgctttcgg cgtcgggccg tcgataacaa
    25681 agtattcgag atattcactc tcaacgctga actgcacttt ggagactttc tccgatccca
    25741 cttcaaaaga gacacactgg ggatgattga ccagtacccc ataaccacgg ttagtcatgt
    25801 agaacgggat atttttatac gcctgttcag tacttgtgcc gccgtcccgg ttccaggtct
    25861 ctaccgtctg gccattgcgc accagggcag taaagcgctc tcccagaccg taaactgttt
    25921 cgccaacgcc aagatcaagc cgctcaaaca tataattgcg ttgattattc gtgtcctgca
    25981 cgtagccatt atttttcacc tgactaccgg taatacgttc gccgttgcgc agaaaatcca
    26041 gtgaccagaa ctcacctttg ctgacacgcg cgcttaagtt gccactttta aactcagcat
    26101 aacgttctgt gttttcgatt gtgaccttca cgtcctgcaa aatattgagc ggataatgag
    26161 gaccgttatt cagcgccccc tgaaaatgct caatccgcac accgacaata ccttcctgtg
    26221 gggagaaaaa gcgcagcgta aataaaggcg tatcaagctg ccaggtacgt tcacgcacat
    26281 cacggggggc agcatagacc accatttcat tatcctgctg ttcaacctcg aatacctgaa
    26341 gcgggtgaat caaattgagg ccaggttgaa tcaaccagtt tccatcgcta attttcattg
    26401 ccgttcctta gttctgcact tcttgagagg tgaattgttg ctggcaacgg cgtttaccct
    26461 gagccagctg ttccataacg gttttcagat tgtgcgtcgt gagtgagtag tagcgtttag
    26521 cgataatcgc gctcagcaaa taacagatcg ccggaacaat cgtgaatagc gcaataatga
    26581 tgctaatcgt ggcgctgttc tgcgcttttt ctgccgcatc atatccgcca taagccagca
    26641 tccagccgat aagagcgccg ccgaaggcca accccagttt gagcacaaac agcgtgccag
    26701 caaaactgat cccggtcagg cgtttaccat tgcaccactc gccgtagtcg acggtatcga
    26761 acatcattac ccactggata ggtgtcacca gttgatgcaa cacaccaatc acgaagatga
    26821 agacaaacat agtgatgctg gcctgcatgg gaacaaagaa catcgcgagg ctaatcactg
    26881 ccagcagggc gttcgtccac cagaagatag tgactttaca tttccagtcg gtcagaggtt
    26941 ttgccagtgc ggaaccaatc aggttaccca cgcaataagt ggtgagaaaa gcgacaaaca
    27001 cttccggcgt gcccaaaatc catgtgacgt aatacatcat cgccccaccg cgtacgcaca
    27061 ccgccaggat attgaaaatg gttagtaaac cgacaatccg ccactggtcg ttttgccaga
    27121 tatcacgtaa atcttcccgc atagacgttg ttgtaggtgg tgcttcaacg cgttctttag
    27181 tggtgaagaa acaaaatgcc agcatcatga atgccaccac ggaaaggacc acgataccgc
    27241 cctggaaacc gagtggttta ttatcaccgc caattaaatt aaccagtggc atcatcagaa
    27301 cagtagaaag catgcctccc gcggtcgcca gcacaaaacg ccaggattgc agcgagatac
    27361 gctgagtcgg gtcattggtg attacaccac ccaatgcgca gtaagggata ttgacgacgg
    27421 tataaagtaa ggtaagttag ggtgtaagta attgctgcat agatcatttt gccgttcata
    27481 ctgagatctg gcgtgctata ggccagtaca cagacgatcc cgaatggcag tgcgccaaac
    27541 agtacccacg gacgaaattt accccagcga gagcgcgttc gatcggccaa caaccccatg
    27601 caaggatcgg aaatcgcatc cagtgcacga gcgaccaaaa acatggttcc gacaaatccg
    27661 gcaggaatgc caaaaatatc ggtataaaag aacatcatat ataacattac gttatcgaaa
    27721 ataatgtggc tggcggcgtc tcccatgcca taaccaattt tctctttaac ggacaacact
    27781 tcactcctca tctgcttttc cttgatgcta taggggtgct gagaccgata acgcattttg
    27841 taaaccatcc ctgtgagagc gaatattccg atatctggtt attaatttat gattcttgtt
    27901 ttatgtgatc gtggtagcgt taattccgct catatatcat tgtaaaatat gggttttata
    27961 taaattataa tgctttcgtg ataatacgct gcgtttatta ggcggaaaaa actgatctgg
    28021 gggatgtaga aactcaagga agtagctata atgcgccccg cctccatgta gcaatcgagg
    28081 cgcggaagat cgtcgtctcc ggtgaggcgg ctggacttca aatccagttg gggccgccag
    28141 cggtcccggg caggttcgac tcctgtgatc ttccgccagt ttacctcttc tgaactctcc
    28201 caatgtcaat aaatctcttc taatcccttg tatatcagta tttatttgta tcctgaggtc
    28261 aacctacttc accccgtatc aagtttcttt tgggggtact ttagggggtg ttatttccta
    28321 ttgaaataca agcaccccca taataggaaa aatgtgatac ataacaacgg cttttaacaa
    28381 tatctttgtt atacaagggg tgtattgggg gtatcaggtt agttactggc agaaatcccc
    28441 ctcaggaaca tgaattcatc aaaagccgga ggatgtcatg gcattaacga tatcaaagtg
    28501 aaaaccgcaa aacccaaaga caaaccctat aagcttgctg atggcggcgg tatgtatcta
    28561 ctgattaaca ctaatggttc taaatactgg cgcatgaagt accgctttgc tggtaaagaa
    28621 aagatgcttt ctattggcgt ataccccgat gtaacgctag ccgatgctcg cgagaaacgc
    28681 agtgaagcca ggaaactcct cgctgctgga ggcgatccag gggaggcaaa aaaggaagaa
    28741 aaaatcgcgc agcaaatgag tctgaaaaat acgttcgagg ctattgctcg cgaatggcac
    28801 cagttaaaag ctgatcggtg gtcattacgc tatcgtgatg aaatcattga tacctttgaa
    28861 aaagacattt ttccctacat tggcaagcgc cctattgccg aaatcaaacc aatgaagtta
    28921 ctggaagcac tgcgtaaaat ggaaaaacgt ggagctctgg agaaaatgcg caaggtccgc
    28981 cagcgctgtg gtgaggtgtt ccgctatgca attgttactg gccgggcaga ctataaccca
    29041 gcccccgatc ttgccagtgc tttagctaca ccgaaaaaag tacattttcc ctttcttact
    29101 gctaatgaac ttccccactt cctcaacgat ctggcgggtt ataccggaag tataatcact
    29161 aagacagcta ctcagatcat tatgttaacc ggcgtacgaa cacaagaact gcgttttgca
    29221 cgctgggaag atatcgattt tgagacaaaa ttgtgggaga tcccggcaga agtaatgaag
    29281 atgaagcgac ctcatattgt gccgctctct gagcaggtca ttatgctatt taagcagctt
    29341 gaaccaatct caaaacatca tcccctagtc tttatcggta gaaatgatcc ccgcaaacca
    29401 attagtaagg aaagcattaa ccaagtaatt gaattacttg gatataaggg aagactcaca
    29461 gggcacggtt tcagacacac catgagtaca attctgcatg aacaaggctt taattccgcc
    29521 tggattgaaa tgcagttagc tcatgtggat aaaaattcca tcaggggtac ctataatcat
    29581 gccctgtatc tcgatggccg ccgtgaaatg atgcaatggt acgctgatta cattgattca
    29641 ctctccagcc gggaaagtta agtcagaaat acttttccct gatgttttcc tcaattaagt
    29701 attttatcct ctggcctaca aggctggagg atttttgtta ttatattatt tttttgcgtc
    29761 ttcaaactta ttacaaaaac ataattaaaa acaaattgac atttttaaat tggcaattac
    29821 aaaatacagc cttgcagtta tttcgatacc tttagcaaag gaatagttga ttcagatata
    29881 atttagcatt tcaatcatct gacatcatta taaaaaatga gattcacata aagataaaat
    29941 tattgttctc aaggaaagca tgaaggggct tcgtaagcaa tattcattcg gtgccaagac
    30001 aggacttgat acacagaatc tcaactttta tgctctcaga ctatatcacc acatgtttta
    30061 caggctcaga aaatgaacga tagattatgc tttgaagtgc acgataacaa gggttatttc
    30121 gtatttccgg atacctggtt tggcccattg ctgggggagt ttgaagaagt gcttgatgca
    30181 tacgacgctg atgaaatcag cgagacgagt tacatcaaca aactccgacg ccttgctcaa
    30241 cgggagccgg actttattga tatacacgct catctggcat atgcatttct ggaacagaat
    30301 gccccgcgta aggcactgaa cgccgctctt aaaggactgg cggcaggtaa tcgtatcatc
    30361 ccggaaagtt tctgtggtga aataatctgg atgcatcccg aaaaccggcc ttatctgcgc
    30421 gcgctttatg cagcaattct cgcaaatgtg cacttacagc gccatcagga tgcggttatg
    30481 ctcactgata aaatactggc atacaatcct gaagataatc agggggctcg ctggttgttg
    30541 ggttcagagc tgttacgcac cggcgatcat gagcgagcct tcagtgtact gaaagagcat
    30601 gctgatgaat tttcacctta ctggtatgaa ctcggtctcc tgcattttct gaacggggaa
    30661 catgttaagg ccgccacagc ttttcgccat ggctttgcca caaacactta catcgcagaa
    30721 atgctgtgcg gaaatctgca tccatttcca cttgctgtgt ggcatgactt ctccggctct
    30781 ctggatacgg cagaagatta ctatgcaaca tactcaccat tatggggaca atatccggaa
    30841 gcattgctgt ttgtaaactg gctctataac cattcctccg tgctgcatga acgatcagaa
    30901 atcattaaat gcgccgaaat gctgattcag gaagatgatt tcgaaatatg cgaaagtatt
    30961 ttaagacagc aggagcacct gtggaaaaga attgacaaga cactttctga ggaaattgta
    31021 cagaaatgca gaaatatgaa cggtgaatat atctggccct ggatattgcc cttttccgca
    31081 gcagggatga aacattcaag catccagtat cagtagatat tgcattagtg taactttaaa
    31141 acaagtaata atttattatc caataataac actgtgttcc ttccccaggt gattaccctg
    31201 gggttatttt ctgaaagttt cctttgcaaa aggatagcgt tgtaaattac tttaacgaat
    31261 tactccatga actcattaaa gttctttcgt aatactttat cgtcaaacac gttatagctg
    31321 gcatcaaaaa ccacagattt accctgccag cgggtaatcc cctcctctgg cgttttcggt
    31381 tgaactggta tccccgtcca acacaggcag ccattataaa cagtaaaaaa ttgtattcgc
    31441 tgttatgaaa tcaatagtcc tgacgtactg ttgaatgtgc ttgtgttctg aacctcgtac
    31501 aaccagtaca tttgatatcg aattataaac aaaacattgc atccaacttt tttaatatgg
    31561 acaatagttg tccatacggt ggttttatat tcgataaatc aatggatttc attgagtcac
    31621 aggactggta atggatgaaa atgctttaag gtttgcctca tactggcgca actcgcttgc
    31681 agatgctgag tcaggaaagg gctgttttga acggaaagac gcccaaaatt tcactcactg
    31741 gcatgggata gcggcgggac gtcttgacga agcgattgtc aataaatttt ttaagggaga
    31801 aaaagacgat gtcgaaactg tcaatgtcat cttacgccca aaagtttatt tccggttac
//
LOCUS       DAJTRC010000042        30790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.042, whole genome shotgun sequence.
ACCESSION   DAJTRC010000042 DAJTRC010000000
VERSION     DAJTRC010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.042"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            379..2037
                     /locus_tag="OO357_002054"
     CDS             379..2037
                     /locus_tag="OO357_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent endonuclease"
                     /protein_id="HCR5887007.1"
                     /translation="MILERVEIVGFRGINRLSLMLEQNNVLIGENAWGKSSLLDALTL
                     LLSPESDLYHFERDDFWFPPGDINGREHHLHIILTFRESLPGRHRVRRYRPLEACWTP
                     CTDGYHRIFYRLEGESAEDGSVMTLRSFLDKDGHPIDVEDINDQARHLVRLMPVLRLR
                     DARFMRRIRNGTVPNVPNVEVTARQLDFLARELSSHPQNLSDGQIRQGLSAMVQLLEH
                     YFSEQGAGQARYRLMRRRASNEQRSWRYLDIINRMIDRPGGRSYRVILLGLFATLLQA
                     KGTLRLDKDARPLLLIEDPETRLHPIMLSVAWHLLNLLPLQRIATTNSGELLSLTPVE
                     HVCRLVRESSRVAAWRLGPSGLSTEDSRRISFHIRFNRPSSLFARCWLLVEGETETWV
                     INELARQCGHHFDAEGIKVIEFAQSGLKPLVKFARRMGIEWHVLVDGDEAGKKYAATV
                     RSLLNNDREAEREHLTALPALDMEHFMYRQGFSDVFHRVAQIPENVPMNLRKIISKAI
                     HRSSKPDLAIEVAMEAGRRGVDSVPTLLKKMFSRVLWLARGRAD"
     gene            complement(2034..2990)
                     /locus_tag="OO357_002055"
     CDS             complement(2034..2990)
                     /locus_tag="OO357_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001322270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VirK/YbjX family protein"
                     /protein_id="HCR5887008.1"
                     /translation="MNMSQLTERTFTPSESLSSLSLFLSLARGQCRPGKFWHRCSFRQ
                     KFLLRSLIMPRLSVEWMNELSHWPNLNVLLTRQPRLPVRLHRPYLAANLSRKQLLEAL
                     RYHYALLRGCMSAEEFSLYLNTPGLQLAKLEGKNGEQFTLELTMMISMDKEGDSTILF
                     RNSEGIPLAEITFTLCEYQGKRTMFIGGLQGAKWEIPHQEIQNATKACHGLFPKRLVM
                     EAACLFAQRLQVEQIIAVSNETHIYRSLRYRDKEGKIHADYNAFWESVGGVCDAERHY
                     RLPAQIARKEIAEIASKKRAEYRRRYEMLDAIQPQMATMFRG"
     gene            3141..4256
                     /gene="macA"
                     /locus_tag="OO357_002056"
     CDS             3141..4256
                     /gene="macA"
                     /locus_tag="OO357_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308991.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide transporter subunit MacA"
                     /protein_id="HCR5887009.1"
                     /translation="MKKRKTVKKRYVIALVIVIAGLITLWRILNAPVPTYQTLIVRPG
                     DLQQSVLATGKLDALRKVDVGAQVSGQLKTLSVAIGDKVKKDQLLGVIDPEQAENQIK
                     EVEATLMELRAQRQQAEAELKLARVTYSRQQRLAQTQAVSLQDLDTAATEMAVKQAQI
                     GTIDAQIKRNQASLDTAKTNLDYTRIVAPMAGEVTQITTLQGQTVIAAQQAPNILTLA
                     DMSTMLVKAQVSEADVIHLKPGQKAWFTVLGDPLTRYEGQIKDVLPTPEKVNDAIFYY
                     ARFEVPNPNGLLRLDMTAQVHIQLTDVKNVLTIPLSALGDPVGDNRYKVKLLRNGETR
                     EREVTIGARNDTDVEIVKGLEAGDEVVIGEAKPGAAQ"
     gene            4253..6199
                     /gene="macB"
                     /locus_tag="OO357_002057"
     CDS             4253..6199
                     /gene="macB"
                     /locus_tag="OO357_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrolide ABC transporter ATP-binding
                     protein/permease MacB"
                     /protein_id="HCR5887010.1"
                     /translation="MTPLLELKDIRRSYPAGDEQVEVLKGISLNIYAGEMVAIVGASG
                     SGKSTLMNILGCLDKATSGTYRVAGQDIATLDADALAQLRREHFGFIFQRYHLLPHLT
                     VEQNVEVPAVYAGLERKQRLLRAQELLQRLGLEDRTEYYPAQLSGGQQQRVSIARALM
                     NGGQVILADEPTGALDSHSGEEVMAILHQLRDRGHTVIIVTHDPQVAAQAERVIEIRD
                     GEIVRNPPAIEKVNVAGGTEPVVNTVSGWRQFVSGFNEALTMAWRALAANKMRTLLTM
                     LGIIIGIASVVSIVVVGDAAKQMVLADIRSIGTNTIDVYPGKDFGDDDPQYQQALKYD
                     DLIAIQKQPWVASATPAVSQNLRLRYNNVDVAASANGVSGDYFNVYGMTFSEGNTFNQ
                     EQLNGRAQVVVLDSNTRRQLFPHKADVVGEVILVGNMPARVIGVAEEKQSMFGSSKVL
                     RVWLPYSTMSGRVMGQSWLNSITVRVKEGFDSAEAEQQLTRLLSLRHGKKDFFTWNMD
                     GVLKTVEKTTRTLQLFLTLVAVISLVVGGIGVMNIMLVSVTERTREIGIRMAVGARAS
                     DVLQQFLIEAVLVCLVGGALGITLSLLIAFTLQLFLPGWEIGFSPLALLLAFLCSTAT
                     GILFGWLPARNAARLDPVDALARE"
     gene            complement(6272..6496)
                     /gene="cspD"
                     /locus_tag="OO357_002058"
     CDS             complement(6272..6496)
                     /gene="cspD"
                     /locus_tag="OO357_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspD"
                     /protein_id="HCR5887011.1"
                     /translation="MEKGTVKWFNNAKGFGFICPEGGGEDIFAHYSTIQMDGYRTLKA
                     GQSVQFDVHQGPKGNHASVIVPVEVEAAVA"
     gene            6819..7139
                     /gene="clpS"
                     /locus_tag="OO357_002059"
     CDS             6819..7139
                     /gene="clpS"
                     /locus_tag="OO357_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005128368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HCR5887012.1"
                     /translation="MGKTNDWLDFDQLAEEKVRDALKPPSMYKVILVNDDYTPMEFVI
                     DVLQKFFSYDVERATQLMLAVHYQGKAICGVFTAEVAETKVAMVNKYARENEHPLLCT
                     LEKA"
     gene            7170..9446
                     /gene="clpA"
                     /locus_tag="OO357_002060"
     CDS             7170..9446
                     /gene="clpA"
                     /locus_tag="OO357_002060"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706768.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HCR5887013.1"
                     /translation="MLNQELELSLNMAFARAREHRHEFMTVEHLLLALLSNPSAREAL
                     EACSVDLVALRQELEAFIEQTTPVLPASEEERDTQPTLSFQRVLQRAVFHVQSSGRNE
                     VTGANVLVAIFSEQESQAAYLLRKHEVSRLDVVNFISHGTRKDEPTQSSDPGSQPNSE
                     EQAGGEERMENFTTNLNQLARVGGIDPLIGREKELERAIQVLCRRRKNNPLLVGESGV
                     GKTAIAEGLAWRIVQGDVPEVMADCTIYSLDIGSLLAGTKYRGDFEKRFKALLKQLEQ
                     DTNSILFIDEIHTIIGAGAASGGQVDAANLIKPLLSSGKIRVIGSTTYQEFSNIFEKD
                     RALARRFQKIDITEPSIEETVQIINGLKPKYEAHHDVRYTAKAVRAAVELAVKYINDR
                     HLPDKAIDVIDEAGARARLMPVSKRKKTVNVADIESVVARIARIPEKSVSQSDRDTLK
                     KLSDRLKMLVFGQDKAIEALTEAIKMARAGLGHEHKPVGSFLFAGPTGVGKTEVTVQL
                     SKALGIELLRFDMSEYMERHTVSRLIGAPPGYVGFDQGGLLTDAVIKHPYAVLLLDEI
                     EKAHPDVFNILLQVMDNGTLTDNNGRKADFRNVVLVMTTNAGVRETERKSIGLIHQDN
                     STDAMEEIKKIFTPEFRNRLDNIIWFDHLSTDVIHQVVDKFIVELQVQLDQKGVSLEV
                     SQEARNWLAEKGYDRAMGARPMARVIQDNLKKPLANELLFGSLVDGGQVTVALDKEKN
                     ELTYGFQSAQKHKAEAAH"
     gene            complement(9849..9936)
                     /locus_tag="OO357_002061"
     tRNA            complement(9849..9936)
                     /locus_tag="OO357_002061"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9900..9902),aa:Ser,seq:gga)
     gene            complement(10190..10408)
                     /gene="infA"
                     /locus_tag="OO357_002062"
     CDS             complement(10190..10408)
                     /gene="infA"
                     /locus_tag="OO357_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001040186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HCR5887014.1"
                     /translation="MAKEDNIEMQGTVLETLPNTMFRVELENGHVVTAHISGKMRKNY
                     IRILTGDKVTVELTPYDLSKGRIVFRSR"
     gene            complement(10693..11397)
                     /gene="aat"
                     /locus_tag="OO357_002063"
     CDS             complement(10693..11397)
                     /gene="aat"
                     /locus_tag="OO357_002063"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HCR5887015.1"
                     /translation="MRLVQLSRHSIAFPSPEGALREPNGLLALGGDLSPARLLMAYQR
                     GIFPWFSPGDPILWWSPDPRAVLWPESLHISRSMKRFHKRSPYRVTMNYAFGQVIEGC
                     ASDREEGTWITRGVVEAYHRLHELGHAHSIEVWREDELVGGMYGVAQGTLFCGESMFS
                     RMENASKTALLVFCEEFIGHGGKLIDCQVLNDHTASLGACEIPRRDYLNYLNQMRLGR
                     LPNNFWVPRCLFSPQE"
     gene            complement(11439..13160)
                     /gene="cydC"
                     /locus_tag="OO357_002064"
     CDS             complement(11439..13160)
                     /gene="cydC"
                     /locus_tag="OO357_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter ATP-binding
                     protein/permease CydC"
                     /protein_id="HCR5887016.1"
                     /translation="MRALLPYLALYKRHKWMLSLGIVLAIVTLLASIGLLTLSGWFLS
                     ASAVAGVAGLYSFNYMLPAAGVRGAAITRTAGRYFERLVSHDATFRVLQHLRIYTFSK
                     LLPLSPAGLARYRQGELLNRVVADVDTLDHLYLRVISPLVGAFVVIMVVTIGLSFLDF
                     TLAFTLGSVMLLTLFLMPPLFYRAGKSTGQNLTHLRGQYRQQLTAWLQGQAELTIFGA
                     SDRYRTQLENTEIQWLEAQRRQSELTALSQAIMLLIGALAVILMLWMASGGVGGNAQP
                     GALIALFVFCALAAFEALAPVTGAFQHLGQVIASAVRISDLTDQKPEVTFPDTQTRVA
                     DRVSLTLRDVQFTYPEQSQQALKGISLQVNAGEHIAILGRTGCGKSTLLQLLTRAWDP
                     QQGEILLNDSPIASLNEAALRQTISVVPQRVHLFSATLRDNLLLASPGSSDEALSEIL
                     RRVGLEKLLEDAGLNSWLGEGGRQLSGGELRRLAIARTLLHDAPLVLLDEPTEGLDAT
                     TESQILELLAKMMREKTVLMVTHRLRGLSRFQQIIVMDNGQIIEQGTHAELLARQGRY
                     YQFKQGL"
     gene            complement(13161..14927)
                     /gene="cydD"
                     /locus_tag="OO357_002065"
     CDS             complement(13161..14927)
                     /gene="cydD"
                     /locus_tag="OO357_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/glutathione ABC transporter
                     permease/ATP-binding protein CydD"
                     /protein_id="HCR5887017.1"
                     /translation="MNKSRQKELTRWLKQQSVISQRWLNISRLLGFVSGILIIAQAWF
                     MARILQHMIMENIPREALLLPFTLLVLTFVLRAWVVWLRERVGYHAGQHIRFAIRRQV
                     LDRLQQAGPAWIQGKPAGSWATLVLEQIDDMHDYYARYLPQMALAVSVPLLIVVAIFP
                     SNWAAALILLGTAPLIPLFMALVGMGAADANRRNFLALARLSGHFLDRLRGMETLRIF
                     GRGEAEIESIRSASEDFRQRTMEVLRLAFLSSGILEFFTSLSIALVAVYFGFSYLGEL
                     DFGHYDTGVTLAAGFLALILAPEFFQPLRDLGTFYHAKAQAVGAADSLKTFMETPLAH
                     PQRGEAELALTDPLTIEAEDLFITSPEGKTLAGPLNFTLPAGQRAVLVGRSGSGKSSL
                     LNALSGFLSYQGSLRINGIELRDLSPESWRKHLSWVGQNPQLPAAKLRDNVLLARPDA
                     SEQELQAALDNAWVSEFLPLLPQGVDTPVGDQAARLSVGQAQRVAVARALLNPCSLLL
                     LDEPAASLDAHSEQRVMEALNAASLRQTTLMVTHQLEDLADWDVIWVMQDGRIIEQGR
                     YAELSVAGGPFATLLAHRQEEI"
     gene            complement(15050..16015)
                     /gene="trxB"
                     /locus_tag="OO357_002066"
     CDS             complement(15050..16015)
                     /gene="trxB"
                     /locus_tag="OO357_002066"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HCR5887018.1"
                     /translation="MGTTKHSKLLILGSGPAGYTAAVYAARANLQPVLITGMEKGGQL
                     TTTTEVENWPGDPNDLTGPLLMARMHEHATKFETEIIFDHINKVDLQNRPFRLNGDNG
                     EYTCDALIIATGASARYLGLPSEEAFKGRGVSACATCDGFFYRNQKVAVIGGGNTAVE
                     EALYLSNIASEVHLIHRRDGFRAEKILIKRLMDKVENGNIILHTNRTLEEVTGDQMGV
                     TGVRLRDTQNSDNIESLDVAGLFVAIGHSPNTAIFEGQLELENGYIKVQSGIHGNATQ
                     TSIPGVFAAGDVMDHIYRQAITSAGTGCMAALDAERYLDGLADAK"
     gene            16559..17053
                     /gene="lrp"
                     /locus_tag="OO357_002067"
     CDS             16559..17053
                     /gene="lrp"
                     /locus_tag="OO357_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001591745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-responsive transcriptional regulator
                     Lrp"
                     /protein_id="HCR5887019.1"
                     /translation="MVDSKKRPGKDLDRIDRNILNELQKDGRISNVELSKRVGLSPTP
                     CLERVRRLERQGFIQGYTALLNPHYLDASLLVFVEITLNRGAPDVFEQFNTAVQKLEE
                     IQECHLVSGDFDYLLKTRVPDMSAYRKLLGETLLRLPGVNDTRTYVVMEEVKQSNRLV
                     IKTR"
     gene            17188..21216
                     /gene="ftsK"
                     /locus_tag="OO357_002068"
     CDS             17188..21216
                     /gene="ftsK"
                     /locus_tag="OO357_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HCR5887020.1"
                     /translation="MSQEYTEDKEVTLTKLSSGRRLLEALLILIVLFAVWLMAALLSF
                     NPSDPSWSQTAWHEPIHNLGGMPGAWLADTLFFIFGVMAYTIPVIIVGGCWFAWRHQS
                     SDEYIDYFAVSLRIIGVLALILTSCGLAAINADDIWYFASGGVIGSLLSTTLQPLLHS
                     SGGTIALLCVWAAGLTLFTGWSWVTIAEKLGGWILNILTFASNRTRRDDTWVDEDEYE
                     DDEEYEDENHGKQHESRRARILRGALARRKRLAEKFINPMGRQTDAALFSGKRMDDDE
                     EITYTARGVAADPDDVLFSGNRATQPEYDEYDPLLNSAPITEPVAVAAAATTATQSWA
                     APVEPVTQTPPVASVDVPPSQPTVAWQPVPGPQTGEPVIAPAPEGYPQQSQYAQPAVQ
                     YNEPLQQPVQPQQPYYAPAAEQPAQQPYYAPAPEQPVAGNAWQAEEQQSTFAPQSTYQ
                     TEQTYQQPAAQEPLYQQPQPVEQQPVVEPEPVVEETKPARPPLYYFEEVEEKRARERE
                     QLAAWYQPIPEPVKEPEPIKSSLKAPSVAAVPPVEAAAAVSPLASGVKKATLATGAAA
                     TVAAPVFSLANSGGPRPQVKEGIGPQLPRPKRIRVPTRRELASYGIKLPSQRAAEEKA
                     REAQRNQYDSGDQYNDDEIDAMQQDELARQFAQTQQQRYGEQYQHDVPVNAEDADAAA
                     EAELARQFAQTQQQRYSGEQPAGANPFSLDDFEFSPMKALLDDGPHEPLFTPIVEPVQ
                     QPQQPVAPQQQYQQPQQPVAPQQQYQQPQQQVAPQPQYQQPQQPVAPQQQYQQPQQPV
                     APQPQYQQPQQPVAPQPQYQQPQQPVAPQPQDTLLHPLLMRNGDSRPLHKPTTPLPSL
                     DLLTPPPSEVEPVDTFALEQMARLVEARLADFRIKADVVNYSPGPVITRFELNLAPGV
                     KAARISNLSRDLARSLSTVAVRVVEVIPGKPYVGLELPNKKRQTVYLREVLDNAKFRD
                     NPSPLTVVLGKDIAGEPVVADLAKMPHLLVAGTTGSGKSVGVNAMILSMLYKAQPEDV
                     RFIMIDPKMLELSVYEGIPHLLTEVVTDMKDAANALRWCVNEMERRYKLMSALGVRNL
                     AGYNEKIAEADRMMRPIPDPYWKPGDSMDAQHPVLKKEPYIVVLVDEFADLMMTVGKK
                     VEELIARLAQKARAAGIHLVLATQRPSVDVITGLIKANIPTRIAFTVSSKIDSRTILD
                     QAGAESLLGMGDMLYSGPNSTLPVRVHGAFVRDQEVHAVVQDWKARGRPQYVDGITSD
                     SESEGGAGGFDGAEELDPLFDQAVQFVTEKRKASISGVQRQFRIGYNRAARIIEQMEA
                     QGIVSEQGHNGNREVLAPPPFD"
     gene            21371..21982
                     /gene="lolA"
                     /locus_tag="OO357_002069"
     CDS             21371..21982
                     /gene="lolA"
                     /locus_tag="OO357_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010354356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein chaperone LolA"
                     /protein_id="HCR5887021.1"
                     /translation="MKKIAITCALLSSLVASSVWADAASDLKSRLDKVSSFHASFTQK
                     VTDGSGAAVQEGQGDLWVKRPNLFNWHMTQPDESILVSDGKTLWFYNPFVEQATATWL
                     KDATGNTPFMLIARNQSSDWQQYNIKQNGDDFVLTPKASNGNLKQFTINVGRDGTIHQ
                     FSAVEQDDQRSSYQLKSQQNGAVDAAKFTFTPPQGVTVDDQRK"
     gene            21993..23336
                     /gene="rarA"
                     /locus_tag="OO357_002070"
                     /gene_synonym="mgsA"
     CDS             21993..23336
                     /gene="rarA"
                     /locus_tag="OO357_002070"
                     /gene_synonym="mgsA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein
                     RarA"
                     /protein_id="HCR5887022.1"
                     /translation="MSNLSLDFSDNTFQPLAARMRPENLAQYIGQQHLLAAGKPLPRA
                     IEAGHLHSMILWGPPGTGKTTLAEVIARYANADVERISAVTSGVKEIREAIERARQNR
                     NAGRRTILFVDEVHRFNKSQQDAFLPHIEDGTITFIGATTENPSFELNSALLSRARVY
                     LLKSLSTEDIEQVLTQAMEDKTRGYGGQDIVLPDDTRRAIAELVNGDARRALNTLEMM
                     ADMAEVYDSGKRVLKPELLTEIAGERSARFDNKGDRFYDLISALHKSVRGSAPDAALY
                     WYARIITAGGDPLYVARRCLAIASEDVGNADPRAMQVAIAAWDCFTRVGPAEGERAIA
                     QAIVYLACAPKSNAVYTAFKAALADARERPDYDVPVHLRNAPTKLMKEMGYGQEYRYA
                     HDEANAYAAGEVYFPPEIAQTRYYFPTNRGLEGKIGEKLAWLAEQDQNSPIKRYR"
     gene            23427..24719
                     /gene="serS"
                     /locus_tag="OO357_002071"
     CDS             23427..24719
                     /gene="serS"
                     /locus_tag="OO357_002071"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000887397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HCR5887023.1"
                     /translation="MLDPNLLRNEPDAVAEKLARRGFKLDVDKLGALEERRKVLQVKT
                     ENLQAERNSRSKSIGQAKARGEDIEPLRLEVNKLGEELDAAKAELDALQAEIRDIALT
                     IPNLPADEVPVGKDENDNVEVSRWGTPREFDFEVRDHVTLGEMHSGLDFAAAVKLTGS
                     RFVVMKGQIARMHRALSQFMLDLHTEQHGYSENYVPYLVNQDTLYGTGQLPKFAGDLF
                     HTRPLEEEADTSNYALIPTAEVPLTNLVRGEIIDEDDLPIKMTAHTPCFRSEAGSYGR
                     DTRGLIRMHQFDKVEMVQIVRPEDSMAALEEMTGHAEKVLQLLGLPYRKIILCTGDMG
                     FGACKTYDLEVWIPAQNTYREISSCSNVWDFQARRMQARCRSKSDKKTRLVHTLNGSG
                     LAVGRTLVAVMENYQQADGRIEVPEVLRPYMNGLEYIG"
     gene            24959..27402
                     /gene="dmsA"
                     /locus_tag="OO357_002072"
                     /pseudo
     CDS             24959..27402
                     /gene="dmsA"
                     /locus_tag="OO357_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415414.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit A"
     gene            27413..28030
                     /gene="dmsB"
                     /locus_tag="OO357_002073"
     CDS             27413..28030
                     /gene="dmsB"
                     /locus_tag="OO357_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000213046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase subunit B"
                     /protein_id="HCR5887024.1"
                     /translation="MTTQYGFFIDSSRCTGCKTCELACKDYKDLTPEVSFRRIYEYAG
                     GDWQEDNGVWHQNVFAYYLSISCNHCEDPACTKVCPSGAMHKREDGFVVVDEDVCIGC
                     RYCHMACPYGAPQYNETKGHMTKCDGCYDRVAEGKKPICVESCPLRALDFGPIDELRK
                     KHGDLAAVAPLPRAHFTKPNIVIKPNANSRPTGDTTGYLANPKEV"
     gene            28032..28823
                     /locus_tag="OO357_002074"
     CDS             28032..28823
                     /locus_tag="OO357_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706781.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethyl sulfoxide reductase anchor subunit"
                     /protein_id="HCR5887025.1"
                     /translation="MGSGWHEWPLMIFTVFGQCVAGGFIVLALALLKGDLRAEAQQRV
                     IACMFGLWVLMGIGFIASMLHLGSPMRAFNSLNRVGASALSNEIASGSIFFAVGGIGW
                     LLAMLKKLSPALRTLWLVVTMVLGVVFVWMMVRVYNSIDTVPTWYSIWTPMGWAMRLL
                     PAISVLALVVSGVMSVMQGTELATIHSSVQQAAALVPDYGALMSWRIVLLAVALCLWI
                     APQLKGYQPAVPLLSVSFILLLAGELIGRGVFYGLHMTVGMAVAS"
     gene            complement(28859..29485)
                     /locus_tag="OO357_002075"
     CDS             complement(28859..29485)
                     /locus_tag="OO357_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HCR5887026.1"
                     /translation="MTKPYVRLDKNDAAVLLVDHQAGLLSLVRDIEPDKFKNNVLALG
                     DLAKYFNLPTILTTSFETGPNGPLVLELKAQFPDAPYIARPGNINAWDNEDFVKAVKA
                     TGKKQLIIAGVVTEVCVAFPALSAIEEGFDVFVVTDASGTFNEITRHSAWDRMSQAGA
                     QLMTWFGVACELHRDWRNDIEGLATLFSNHIPDYRNLMTSYDTLTKQK"
     gene            29800..>30790
                     /locus_tag="OO357_002076"
     CDS             29800..>30790
                     /locus_tag="OO357_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5887027.1"
                     /translation="MSTYTRPVMLLLSGLLLLTLAIAVLNTLVPLWLAQEHMSTWQVG
                     VVSSSYFTGNLVGTLLTGYVIKRIGFNRSYYLSSFIFAAGCAGLGLMIGFWSWLAWRF
                     VAGVGCAMIWVVVESALMCSGTSRNRGRLLAAYMMVYYVGTFLGQLLVSKVSTELMSV
                     LPWVTGLTLAGILPLLFTRVLNQQAENHDSTSITAMLKLRQARLGVNGCIISGIVLGS
                     LYGLMPLYLNHKGVSNASIGFWMAVLVSAGILGQWPIGRLADKFGRLLVLRVQVFVVI
                     LGSIAMLSQAAMAPALFILGAAGFTLYPVAMAWACEKVEHHQLVAMNQALLLSY"
BASE COUNT         7605 a         7559 c         8297 g         7329 t
ORIGIN      
        1 atagggtcgt cgtaaaaaat gacgacggaa ataatgcgcg gctattttaa aaacgaaggc
       61 gagtcattca ccagataaat aaatccagta aatttgattt agggcaacag cgggttgccc
      121 catatagtca tttgtctgat tgacagtgta gtgcaagcaa aagatttaat cctttaggcg
      181 taataaaaaa taatttatca tgctaattat ttgattttgt tgtttttgcg ggcttatcag
      241 caagagggag tataacgcga ttattcgctc atttttcaga caattgccat gcttaaatgt
      301 gatgtcatca cgtattagca aggcctttcc cgttatactg cgagcgtaaa ggataagtca
      361 catatttctg gaggggatat gattcttgag cgcgttgaaa ttgtgggttt tcgcggtatc
      421 aaccgtttgt cgttgatgct cgaacaaaac aacgtcctga ttggggagaa cgcgtggggt
      481 aaatccagct tgctggacgc cttaaccctg ctgctatcgc cagaatcaga tctctaccat
      541 tttgagcgcg acgatttctg gttcccgccg ggagatatca acgggcgaga acatcatctg
      601 catattattt tgaccttccg cgaatcgctg ccaggccgac atcgggttcg ccgttatcgg
      661 ccgctggaag cgtgctggac gccatgcacc gatggctatc accgtatttt ttatcgtctg
      721 gaaggggaga gtgcggaaga cggcagcgtg atgacactgc gcagttttct cgataaagac
      781 ggacatccga ttgatgttga ggatattaac gatcaggcac gccatctggt gcgtttaatg
      841 ccggtgctgc gcttgcgtga tgcccgtttt atgcgccgta ttcgtaacgg cacggtgcca
      901 aatgtcccca atgtggaagt caccgcgcgc cagctcgatt tcctcgcccg tgagttgtcc
      961 tcacatccgc aaaatctctc tgatggacag attcgtcagg gactttccgc aatggtgcag
     1021 ttgcttgagc attatttctc tgagcagggg gctggacagg cgcgatatcg tttaatgcgg
     1081 cggcgggcca gcaatgagca acgaagctgg cgctatctgg acatcatcaa ccggatgatt
     1141 gaccgacctg gagggcgctc gtatcgggtt attttgctcg gcctgtttgc tactttattg
     1201 caggcaaaag gcacattgcg actggataaa gacgcgcgtc cgttattgct aattgaagat
     1261 ccagaaaccc gcctgcatcc gattatgctt tccgttgcct ggcatctgtt gaatcttttg
     1321 cctttacagc gtattgctac caccaactct ggcgagctgc tttcgttaac tccagtggaa
     1381 catgtttgcc gcctggtgcg tgaatcttct cgtgttgccg cctggcgtct ggggccaagt
     1441 ggcttgagca ccgaagacag ccgccgcatc tcttttcaca ttcgttttaa tcgtccgtca
     1501 tcgctgtttg ctcgctgctg gttgctggtg gaaggggaaa cggaaacctg ggttatcaat
     1561 gaactggcgc gtcagtgcgg acatcatttt gatgccgaag ggatcaaggt cattgagttt
     1621 gcccagtccg ggctaaagcc actggttaaa tttgcccgcc gaatggggat tgaatggcat
     1681 gtactggtcg atggcgatga agcagggaag aaatatgccg ctacggtacg cagcctgttg
     1741 aataacgatc gggaagccga acgagaacat ttaacggcgt taccggcgct ggatatggaa
     1801 cattttatgt atcgccaggg attttccgat gtgttccacc gcgtggcgca aatcccggaa
     1861 aatgtaccga tgaatctacg caaaattatc tcgaaagcga tccatcgctc ttccaaaccc
     1921 gatcttgcca ttgaagtggc aatggaggca ggacgtcgtg gtgtggactc cgtaccaacg
     1981 ctgctgaaaa aaatgttctc acgcgtgctg tggctggcgc gcggtcgcgc ggattaaccg
     2041 cgaaacatcg tggccatttg tggctgaata gcgtcgagca tctcatagcg ccgacggtat
     2101 tcagcccgtt ttttactggc gatttcggca atctcttttc gtgctatctg tgctggaagg
     2161 cggtaatggc gttcagcatc acatacgccg ccaaccgact cccagaacgc gttgtaatca
     2221 gcatggatct tgccttcttt atcgcgataa cgcaggctgc ggtaaatatg cgtttcattg
     2281 ctgacggcaa taatctgctc tacctgcaaa cgttgggcaa acagacaggc cgcttccatc
     2341 acgaggcgtt tgggaaatag cccgtggcag gctttcgtcg cattctggat ttcctgatgt
     2401 ggaatttccc attttgcgcc ttgcagtccg ccaataaaca tcgttctttt cccctgatat
     2461 tcacacaggg taaacgtgat ctctgccaga ggaatacctt cgctgttgcg gaacaggatt
     2521 gtgctgtcac cttctttatc cattgagatc atcatggtca gctcaagcgt gaactgctca
     2581 ccgtttttgc cttccagttt agccagttgc agcccggggg tattcaaata taagctgaat
     2641 tcttccgccg acatacaccc gcggagtaac gcataatggt aacgtaacgc ctccagcaat
     2701 tgcttacggc taagattcgc ggcaaggtaa gggcgatgca gacgcacagg cagtcgcggc
     2761 tggcgcgtta acaacacatt gagattaggc cagtgggaaa gttcgttcat ccactcaacg
     2821 cttaaacgcg gcataatcaa cgagcgcagc aaaaatttct ggcgaaaact acagcgatgc
     2881 cagaatttac ccggccgaca ctgtccacgt gccagactaa gaaaaagtga caggctgctg
     2941 agagattcag atggcgtaaa ggtccgttca gttagctgcg acatattcat gaaatcaatg
     3001 gttatacatg acgtcgattt caccattgcg tatcttaacc aaacatcaat agtgtgatta
     3061 ctaacgtaaa ttttagggtt ttgttgatat ttcgttgaag ttaatgaccc ggattggcat
     3121 atggagtatt cagaatattt atgaaaaagc ggaaaaccgt gaagaagcgt tacgttattg
     3181 cgctggtgat agtcatcgcc ggactgatta cgttatggag aattcttaac gcacccgtgc
     3241 cgacttatca gacactgatt gtgcgccccg gtgatttaca gcaaagcgtg ctggcgaccg
     3301 gaaagctgga cgcgctgcgt aaggttgacg tgggcgcgca ggtcagcggt cagttgaaaa
     3361 cgctgtcggt ggcgattggc gataaagtaa aaaaagacca gcttttaggg gtcattgatc
     3421 ctgaacaggc tgaaaaccag atcaaggagg tcgaagcaac gctgatggag ctacgcgcgc
     3481 agcggcagca ggcggaagcg gagctgaaac tggcgcgggt gacgtattcc cgtcagcaac
     3541 gtctggcaca aacgcaagcg gtttcactgc aggatctcga taccgccgcg acggagatgg
     3601 ctgtgaaaca ggcgcaaatt ggcaccattg acgcgcaaat caagcgcaat caggcctctc
     3661 tcgatacggc taaaaccaat ctcgattaca cccgtatcgt tgccccgatg gctggggaag
     3721 tgacgcaaat caccactctg caaggccaga cggtgattgc cgcacagcaa gcaccgaaca
     3781 ttctgacgct ggcagatatg agcactatgc tggtaaaagc gcaggtttct gaagcggatg
     3841 taatccacct gaagccgggg caaaaagcct ggtttacggt gcttggcgat ccactgacgc
     3901 gctacgaggg gcaaatcaag gatgtgctac cgacgccgga aaaggtaaac gacgctattt
     3961 tctattacgc ccgttttgaa gtacccaacc ccaatggttt gctgcggctg gatatgaccg
     4021 cgcaagtgca tattcagctc accgatgtga aaaatgtgtt gacgatccct ctgtcggcgt
     4081 taggcgatcc ggttggcgat aatcgttata aagtcaaatt gttgcgtaat ggtgaaacac
     4141 gcgagcgtga agtgacgatt ggcgcacgta acgataccga tgttgagatt gtcaaagggc
     4201 tggaagcggg cgatgaagtg gtgattggtg aggccaaacc aggagctgca caatgacgcc
     4261 tttgctcgaa ttaaaggata ttcgtcgcag ctatcctgcc ggtgatgagc aggttgaggt
     4321 gctgaagggc atcagcctca atatttatgc gggtgagatg gtcgcgattg ttggcgcttc
     4381 gggttccggt aaatcgaccc tgatgaatat tctcggctgc ctggataagg ccaccagcgg
     4441 cacctatcgc gtagccggtc aggatattgc cacgctggac gccgatgcgc tggcgcaact
     4501 gcgccgcgag catttcggct ttattttcca gcgttaccat ttgcttccgc atttaaccgt
     4561 cgagcagaac gttgaagtac ccgccgtcta tgccggtctt gagcggaaac agcgactgct
     4621 tcgtgcccag gagttgctgc aacggctggg gctggaagac cgtacagagt attatccggc
     4681 acagctttcg ggtggtcagc aacagcgcgt cagcatcgcg cgggcattga tgaacggcgg
     4741 tcaggtaatt cttgccgatg aaccgaccgg cgcactggac agccattctg gcgaagaggt
     4801 gatggcgatc ctgcatcagc tgcgcgatcg cgggcatacg gtgattatcg tcacccacga
     4861 tccgcaggtc gctgctcagg ccgagcgggt gatcgaaatt cgcgacggcg aaattgtgcg
     4921 caatcctccc gccattgaaa aagtgaatgt tgctggcggg acggagcctg ttgtcaatac
     4981 agtgtctggc tggcggcagt ttgtcagcgg ttttaacgag gcgctgacga tggcatggcg
     5041 ggcgctggca gcgaataaaa tgcgtacttt actgaccatg ctggggatta ttatcggtat
     5101 tgcgtcggtg gtttccattg tcgtggtggg tgacgccgcc aaacaaatgg tgctggcgga
     5161 tattcgttct attggtacga atactattga tgtctatccc gggaaagatt ttggcgatga
     5221 cgatccgcaa tatcagcagg cgctgaagta cgacgactta atcgccatcc aaaaacaacc
     5281 gtgggtcgcc tcagccacac ctgccgtctc gcaaaacctg cgcctgcgtt ataacaatgt
     5341 tgatgttgct gccagtgcca atggcgtgag cggcgattat tttaatgtct atggcatgac
     5401 cttcagtgaa ggaaacacct ttaatcagga gcagctgaac ggtcgtgcgc aggtcgtggt
     5461 tctcgacagt aatactcgcc gccagctttt cccccataaa gcagatgtgg ttggcgaggt
     5521 gattctggtc ggcaatatgc ccgccagagt cattggtgtg gcggaagaaa aacagtcgat
     5581 gtttggtagc agtaaagtgc tgcgtgtctg gctaccttac agcacgatgt ccgggcgagt
     5641 tatgggccag tcgtggctta actccattac tgtcagggtg aaagaaggat ttgacagcgc
     5701 cgaggcggaa cagcaactca cgcgtttact ttcactgcgc cacggtaaga aggatttctt
     5761 tacctggaac atggacggcg tcttgaaaac tgttgaaaag accacacgta ctttacaact
     5821 gtttctgacg ctggtggcgg tgatttcgct ggtggtgggc ggtattggtg taatgaatat
     5881 tatgctggtg tcagtgaccg agcggacgcg ggaaattggc attcgcatgg ctgtaggtgc
     5941 gcgagcaagc gatgttttgc aacagttcct gatcgaagcc gtactggttt gcctggtcgg
     6001 tggcgcgttg ggaataacac tgtcactgtt aattgctttc accttgcagc ttttcttacc
     6061 cggctgggag attggttttt caccgttggc gctgctgctg gcgtttctct gctcgacggc
     6121 caccgggatt ttatttggct ggttacccgc acgaaatgcg gcacgactgg atccagtaga
     6181 tgctctggca cgggagtaat ttttgagata aaaatgccag ccgatcgggc tggcattttg
     6241 cctttaggat gtacacaatg agacagaaga gctatgcgac tgccgcttct acttcgacgg
     6301 gcacaataac actggcgtga ttgccttttg gcccctggtg gacatcaaac tgaacggatt
     6361 gtccagcttt tagcgttctg taaccatcca tctgaatggt ggaataatga gcgaaaatat
     6421 cttcgccgcc gccttcaggg cagatgaaac caaacccttt ggcattgttg aaccacttaa
     6481 cagtaccctt ttccatgctt cgacatcctt cgcaaatctt atacaagtaa gatggaataa
     6541 accggggtca gagagggggc tgttcaaaac ctcgccaact ctagaaatac aatttagaga
     6601 attagggcga gccgtcaagc atttgacagg ggccaggggg caggtatgaa tcaaaaattt
     6661 gaagcagtta acgctattga caggaatgtg acagatgtcg ctgatgccaa cgatagatga
     6721 tagttatcta tcatgtggag tagattggtc aggcaaataa gctcttgtca gcggcagggc
     6781 gttctgccga taaccgtaac cgaagatgat aactgacaat gggtaaaacg aacgactggc
     6841 tggactttga tcaactggcg gaagaaaaag ttcgcgacgc gctaaaaccg ccatctatgt
     6901 ataaagtgat attagtcaat gatgattaca ctccgatgga gtttgttatt gacgtgttac
     6961 aaaaattctt ttcttatgat gtagaacgtg caacgcaatt gatgctcgct gttcactacc
     7021 aggggaaggc catttgcgga gtctttaccg ccgaggttgc agaaaccaaa gtggcgatgg
     7081 tgaacaagta cgcgagggag aatgagcatc cattgctgtg tacgctagaa aaagcctgaa
     7141 tgcaggcata aaaattgggg gaggtgccta tgctcaatca agaactggaa ctcagtttaa
     7201 atatggcttt cgccagagcg cgcgagcacc gtcatgagtt tatgaccgtc gagcacttgt
     7261 tactggcgct gctcagtaac ccatctgccc gggaggcgct ggaagcgtgt tctgtggatt
     7321 tggttgcgct ccgtcaggaa ctggaagcct ttattgaaca aaccacaccc gttctgcctg
     7381 ccagtgaaga ggagcgcgac acacagccga cgctgagttt tcagcgtgta ctgcaacgtg
     7441 cggtcttcca tgtccagtcc tccggtcgca atgaggttac cggtgcaaac gttctggtcg
     7501 ctatcttcag cgaacaggag tcgcaggcgg catatctgtt gcgtaaacat gaagtcagcc
     7561 gtctcgatgt ggtgaacttt atctctcatg gcacgcgtaa agacgagccg acacagtctt
     7621 ctgatcctgg cagccagcca aacagcgaag aacaagctgg tggggaggaa cgtatggaga
     7681 atttcacgac gaacctgaac cagcttgcgc gtgtgggcgg aatcgaccca ctaattggtc
     7741 gtgagaaaga gctggagcgt gctattcagg ttctctgccg tcgccgtaaa aacaacccgc
     7801 tgctggtggg ggaatctggt gtcggtaaaa ccgcgattgc agaaggtctt gcctggcgaa
     7861 ttgttcaggg tgatgtgccg gaagtgatgg ctgactgtac gatttactct ctcgatatcg
     7921 gttctctgtt agcgggcaca aaatatcgcg gcgactttga aaaacgtttt aaagcgttgc
     7981 tcaagcagct ggagcaggac actaacagca tcctgtttat tgatgagatc cacaccatta
     8041 tcggtgcggg tgcagcgtct ggtggtcagg tcgatgcggc taacctaatc aaaccgttgc
     8101 tctccagcgg taaaattcgc gtaattggtt cgacaaccta tcaggagttc agcaacattt
     8161 tcgagaaaga ccgtgctctg gcgcgtcgct tccagaaaat tgatattact gaaccgtcga
     8221 tcgaagaaac tgttcaaatc atcaatggcc tgaaaccgaa gtatgaagcg caccacgacg
     8281 tgcgttatac cgcaaaagcg gtgcgtgcgg cggtagagct ggcggtgaaa tacattaacg
     8341 atcgtcatct gccggataaa gccattgatg ttatcgacga agcaggcgct cgcgcacgtc
     8401 tgatgccggt aagcaaacgc aagaaaaccg ttaatgtggc ggatattgag tccgtggtgg
     8461 cccgtattgc acgcattcca gagaagagtg tttctcagag tgaccgcgat accctgaaaa
     8521 aactcagcga tcgcctgaaa atgctggtct tcggccagga taaagccatt gaggcgctga
     8581 ctgaagccat taagatggcg cgtgcaggtt taggtcacga acataaaccg gtcggttcgt
     8641 tcctgtttgc cggtcctacc ggggtcggga aaacagaggt gacggtacag ctttcgaaag
     8701 cgttgggcat tgagcttctg cgctttgata tgtccgagta tatggaacgc cataccgtca
     8761 gccgtctgat tggtgcgcct ccgggatacg ttggttttga tcagggcggt ttgctgaccg
     8821 atgcggtaat caagcatcca tatgcggtgc tgctgctgga cgaaatcgag aaagcgcatc
     8881 cggacgtgtt caatattctg ttgcaggtga tggacaacgg tacgctgacc gataacaacg
     8941 gacgcaaagc ggacttccgt aacgtggtgc tggtgatgac cactaacgcc ggggtacggg
     9001 aaactgagcg caaatccatt ggtcttatcc accaggataa cagcaccgat gcgatggaag
     9061 agatcaagaa gatctttaca ccggaattcc gtaaccgtct cgacaacatt atctggttcg
     9121 atcatctgtc aactgacgtg atccatcagg tggtggataa attcatcgtc gagttgcagg
     9181 ttcagctgga tcagaaaggt gtttctctgg aagtgagcca ggaagcgcgt aactggctgg
     9241 ccgagaaagg ttacgaccgg gcaatgggcg cacgtccgat ggcgcgtgtc atccaggaca
     9301 acctgaaaaa accgctcgcc aacgaactgc tgtttggttc gctggtggac ggcggtcagg
     9361 tgacggttgc gctggataaa gagaaaaatg agctgactta cggattccag agtgcacaaa
     9421 agcacaaggc ggaagcagcg cattaatctg attgtcaggt aggttggtga agtccgtaat
     9481 ctcgtcaggg gttacggact ttttatttat ggggtggaac ggtgaaaacc ctatttttgg
     9541 aggtgaaggt aagttgttga taattagtgc tgctggaagg taaggataaa aaagggtgct
     9601 gcaggagaat gggatggttt tgctttatta acaacgggct aaacgtgtag tatttgagtt
     9661 cactgccgta caggcagctt agaaatcgac ggggtgcggt aaaacctttg cgaacgcgtt
     9721 cactgccgta caggcagctt agaaattcac aggtaacata ctccacccgc ccaccatgtt
     9781 cactgccgta cagacagata aaatgcgaaa aaaaagctcg cactttcgta cgagctcttc
     9841 tttaaatatg gcggtgaggg ggggattcga acccccgata cgttgccgta tacacacttt
     9901 ccaggcgtgc tccttcagcc actcggacac ctcaccaaat tgttttgcta ccaaacctca
     9961 tgggtggcaa cggggcgcta ctatagggag ttggagtaaa acggtcaaga agaattttaa
    10021 tgataattat tgtttgctca tactgtaaac aacttgtgca gtatatctac atcgagacaa
    10081 gttatggact tatacttcca aagtacttca tacatatcac aaaataaaaa ggccggttaa
    10141 accgaccttt tactcgttct ttctcttcgc ccatcaggcg gtaaaacaat cagcgactac
    10201 ggaagacaat gcggcctttg ctcaggtcgt acggggtcag ttcaacagtc actttgtcgc
    10261 ccgtcaggat gcggatgtag tttttgcgca ttttaccgga gatgtgtgca gtaaccacgt
    10321 gaccgttttc taactctacg cggaacatgg tattaggcaa cgtttcaaga acggtacctt
    10381 gcatttcaat attgtcttct ttggccatct aatcctctgg ggtatcacta ccgtaatttg
    10441 aaccggcaag ataatgccga agttctgtaa ataagtaaag atttgcgcgc taaatcgcaa
    10501 caaacaggtt cggcacatta ctccgaaaac acacggctaa gccgcaccaa aagcgcaacg
    10561 tataagggag cggtgagata aacgatgggc gttacctgac gcgaaaaatt ccttatcggc
    10621 agcggggtaa tgagcgtaac caactctgcg accgcaatta taacactctg gggagaaatg
    10681 tgccgaaaac attcattctt gtggtgaaaa caagcaccgt ggtacccaga aattattcgg
    10741 caatcgtccg aggcgcattt gattgagata attaaggtaa tcccggcggg gaatttcgca
    10801 ggcaccaagc gatgctgtgt gatcgttaag gacctggcag tcgataagct taccgccatg
    10861 accgataaat tcctcacaga ataccagaag cgccgtttta gacgcatttt ccatccggct
    10921 gaacatggac tcgccacaaa atagcgttcc ctgggccacg ccgtacatac cgccgacaag
    10981 ctcatcttca cgccagactt caatggagtg ggcatgaccg agttcgtgaa ggcgatggta
    11041 ggcttcgacc acgccacgcg tgatccaggt tccttcttcg cgatcgctgg cacagccttc
    11101 aatgacctga ccaaaagcgt aattcatcgt gacacgatag ggcgagcgtt tatgaaatcg
    11161 cttcatacta cggctgatat gcagtgattc tggccatagc accgcgcggg gatcgggcga
    11221 ccaccagagg atggggtcgc ctggagaaaa ccacggaaaa ataccacgct ggtaagccat
    11281 taacaggcgc gcagggctaa gatcgccccc aagtgccagc aggccgttag gctcacgtaa
    11341 tgcgccttcc ggggaaggga aggctattga atggcgagaa agctgaacca agcgcatgac
    11401 cgcaaaactc cacgcaagtc ggatcgttca ataatagctt acaaaccctg cttgaactgg
    11461 taataacgcc cctgtctggc aagcagttct gcgtgagtac cttgctcaat aatttgcccg
    11521 ttgtccatca ctattatttg ttggaaacga gagagtccgc gaagtcgatg ggtgaccatt
    11581 aacaccgttt tctcacgcat catttttgca agcaattcaa ggatctggct ttcggttgtg
    11641 gcatctaagc cttcggtagg ttcatccagc aacaccagtg gcgcatcatg taacagcgta
    11701 cgggcgatag ccagacggcg cagttcacca ccggagagct ggcgtccgcc ttcacctaac
    11761 caactgttga gacctgcatc ctcgagcagc ttctccagac caacgcgacg caagatctcc
    11821 gacagagcct catcactact gccaggcgag gcgagtaaca gattatcacg cagtgtggcg
    11881 ctaaacagat gcactcgctg aggaacaacg ctgatggtct gtcgtagagc cgcttcattc
    11941 aggctggcta tggggctatc gttaagcaaa atctcgccct gttgcgggtc ccatgcgcgg
    12001 gtcagcagtt gtaacagtgt tgatttgccg catccggttc gcccgagaat cgctatatgt
    12061 tccccggcgt ttacctgaag agaaatccct ttaagtgcct gttgggattg ctccggataa
    12121 gtgaactgaa catcccgtaa tgtcagcgaa acgcgatcgg caacacgagt ttgggtatca
    12181 gggaaggtga cctccggttt ttgatccgtt aagtcagaga tacgtacggc agaggcaatg
    12241 acttgcccca gatgctgaaa tgcacccgtt actggtgcca gtgcttcaaa cgcggctaac
    12301 gcacaaaaga caaacagggc aattaatgcg ccgggttgag cattgccgcc aacgccgcca
    12361 gacgccatcc acagcatcag gatcaccgct aacgcgccaa tgagcagcat tatcgcttgc
    12421 gacaatgcgg tcagttcaga ttgacggcgt tgcgcttcca gccattgaat ttctgtattc
    12481 tctagttgcg tgcgataacg atcgctggca ccaaaaatgg tcagctcagc ttgcccttgc
    12541 agccaggccg tcagttgttg gcgatactgt ccgcgaagat gagtcagatt ttgcccggtg
    12601 ctttttcccg cacgataaaa cagcggtggc atcaggaaaa gcgtcagtaa cataacgctg
    12661 cccagcgtaa aggcgagggt gaaatcaagg aaacttaacc cgattgtcac caccataatc
    12721 accacaaaag cgcccaccag cggcgagata acgcgcaggt aaagatgatc gagcgtatca
    12781 acatccgcca ccacgcggtt gagcaattcg ccctgacgat agcgcgccag tccggcaggg
    12841 gagaggggca acaatttgct gaaggtgtaa atgcgcagat gctgcaacac gcggaaagtc
    12901 gcgtcgtgac ttaccagacg ttcaaaataa cgcccggcag tacgggtgat tgcggcacca
    12961 cgcacgcccg cagcgggtag catatagttg aagctgtaca gtccggcaac ccccgcaacc
    13021 gctgaggccg agaggaacca gccggaaagt gtcaacagac cgatactggc gagcagcgtc
    13081 acaattgcca gcacaatacc aagacttaac atccatttat gacgtttata cagtgccaga
    13141 tagggtagca aagcgcgcat ttaaatctcc tcctgacgat gggccagtaa tgtggcgaat
    13201 gggccaccag ccacacttaa ttccgcgtaa cgtccttgct caataatccg gccatcctgc
    13261 ataacccaaa tgacatccca gtcagcaaga tcttctaact ggtgggtgac cattaacgtt
    13321 gtctggcgca gagaggcggc attcagcgcc tccattacgc gctgttcact gtgagcatca
    13381 aggctggcag cgggttcatc caacagtaat agcgaacagg gatttagtaa cgcacgggcc
    13441 accgccacgc gctgcgcctg ccccacggaa aggcgggcag cctggtcgcc aacaggtgta
    13501 tcaacgcctt gtggcaggag cggtagaaac tcgctgaccc aggcgttatc cagcgctgct
    13561 tgtaattctt gttcgctggc atcaggtcgc gccagtagta cgttatcccg caattttgct
    13621 gccggtaatt gtgggttttg cccaacccag gagagatgtt tacgccatga ttctggtgat
    13681 aaatcacgta attctatccc gttgattcgt aatgatccct gatatgagag aaaaccagaa
    13741 agcgcgttca gcagtgagct tttacctgaa ccgctgcggc caaccaacac tgcacgttgg
    13801 cctgctggca aagtaaagtt cagcggtccg gccagcgttt taccttccgg cgacgtgata
    13861 aacagatcct cggcctcaat ggtcagcgga tcggtcaatg ctaattccgc ctcaccgcgc
    13921 tgcggatggg cgagcggggt ttccataaac gttttcagac tgtcagctgc accaacagcc
    13981 tgggctttag catgataaaa cgtaccgaga tcgcgtaatg gctggaaaaa ctctggcgca
    14041 aggatcaggg ccagaaaacc tgcagccagc gtcaccccag tatcgtagtg accaaaatcc
    14101 agctcgccga gataggaaaa accaaagtag accgccacca gagcaattga cagcgaggta
    14161 aaaaattcga gaatgccgga ggataaaaac gccagtcgta gcacttccat tgtccgttgg
    14221 cggaaatctt ccgaagcaga acgaatactt tcaatttcag cttcaccacg accaaaaata
    14281 cgcaatgttt ccatgccgcg caggcgatcg aggaaatgcc cacttaagcg agcaagagcg
    14341 agaaagttac gccggttagc atcggcagcc cccattccaa ccagcgccat aaacaacgga
    14401 attagcggtg ctgtgcccag cagaatgagc gccgcagccc agttagaggg gaagatcgcc
    14461 accacaatca gcaacggcac cgacactgcc agcgccattt gcggcagata gcgtgcatag
    14521 taatcatgca tatcgtcaat ttgctcgagt accagcgtcg cccagctccc cgcaggttta
    14581 ccctgaatcc acgctggccc tgcttgttgc agacggtcga gaacctgacg gcggatggca
    14641 aagcggatat gctgcccggc gtgataaccc acccgttcgc gtaaccagac cacccatgcg
    14701 cgcaatacaa aggtcagaac cagtaacgta aagggaagca gcagggcttc acggggaata
    14761 ttctccataa tcatatgttg cagaattcgc gccatgaacc aggcctgggc aatgatcaat
    14821 atgccgctta caaagcccag cagacgagaa atattcagcc aacgttggga gatgacgctt
    14881 tgctgtttta accagcgggt taactctttt tgacgagatt tattcattgc acgcttagca
    14941 ggtgagttat cagaattatt tgcagagcaa tgttacaacg gggaaaaaat aaaggcgacc
    15001 catagtcgca aggtgtcgcc ttctttactt ttgttactga tttgtaaaat tattttgcgt
    15061 cagctaaacc atcgaggtag cgttccgcat caagtgctgc catgcagcca gtaccggccg
    15121 aagtaatggc ctggcgataa atgtgatcca tcacgtcgcc tgcggcaaag acgccaggaa
    15181 tgctggtctg ggtggcatta ccatgaatac ccgactgtac tttgatgtag ccgttttcca
    15241 gttccagctg cccttcgaaa atcgcagtat tcgggctgtg accgatagca acaaacagac
    15301 cggcaacgtc gagtgactcg atgttatcgc tgttttgcgt atcgcgcaga cgaacgccag
    15361 tgacacccat ttgatcgccg gtcacttctt ccagcgtacg gttggtgtgc agaatgatgt
    15421 tgccgttctc cactttatcc atcagacgct taataaggat tttttccgcg cggaaaccgt
    15481 cacggcggtg aatcagatgc acttccgaag cgatgttaga cagatacagc gcctcttcaa
    15541 ccgcggtatt gccgccgccg atgaccgcaa ctttctggtt gcgatagaag aaaccgtcgc
    15601 aggttgcaca agcagaaacc ccacggcctt taaaggcttc ttcagagggc aggccgagat
    15661 agcgtgcaga agctccggtg gcaataatca gcgcgtcgca agtgtattcg ccgttatcgc
    15721 cattcagacg gaacggacgg ttttgcagat ccaccttgtt gatatgatca aaaatgatct
    15781 cagtttcaaa cttggtggca tgttcgtgca tgcgcgccat taataacgga ccggtcagat
    15841 cgtttggatc accaggccag ttttccactt ccgtggtggt ggtcagttgg ccgccttttt
    15901 ccatgccggt aatcagcaca ggttgcaggt tggcgcgcgc cgcgtagaca gcagcggtgt
    15961 atcccgccgg gcctgaaccc aggataagca gtttactgtg tttggtcgtg cccatgagat
    16021 ccccatagtt gttggcagac aatgggcagg attgtaggga atttacagac gtaaaaaaag
    16081 agtatgacga ttttgttaac aatttgtgca atcggcagca tcgataagca ggtcaaattc
    16141 tcccgtcatt atcacctctg ctacttaaat ttcccgcttt ataagccgat taaatgatga
    16201 ataaacgccc ctgttaatga atatctggca tgttgtacta aaaatcgatg ttttgctttg
    16261 acaatcccct ggtgttttgc gaaaacattc gaggaagaaa aaaacagtat tcttatatgc
    16321 gcataaccat gtatgtaaat accatgttta ccgtgctagt gaaatctacg tatggcgtgg
    16381 acagacgcca ttcgtgatgt cgatagctgc cacaaggcaa cggtcttctc accgtagacc
    16441 caggcattgc gcgccgtgaa tcttcatgat ttcggtctat cgtgacgggt agcgactctg
    16501 aacagtgatg tttcagggtc agacaggagt agggaaggaa tacagagaga caataataat
    16561 ggtagatagc aagaagcgcc ctggcaaaga tctcgaccgt atcgatcgta acattcttaa
    16621 tgagttgcaa aaggatgggc gtatttctaa cgtcgagctt tctaaacgtg tgggactttc
    16681 cccaacgccg tgccttgagc gtgtgcgtcg gctggaaaga caagggttta ttcagggcta
    16741 tacggcgctg cttaaccccc attatctgga tgcatcactt ctggtattcg ttgagattac
    16801 tctgaatcgt ggcgcaccgg atgtgtttga acaattcaat accgctgtac aaaaacttga
    16861 agaaattcag gagtgtcatt tagtatctgg tgatttcgac tacctgttga aaacacgcgt
    16921 gccggatatg tcagcttacc gtaagttgct gggggaaacc ctgctgcgtc tgcctggcgt
    16981 caatgacaca cggacatacg ttgttatgga agaagtcaag cagagtaatc gtctggttat
    17041 taagacgcgc taacacggaa caggtgcaaa atcggcgtat tttgattaca ctcctgttaa
    17101 tccatacagc aacagtactg gggtaacctg gtactgttgt ccgttttagc atcgggcagg
    17161 aaaagcctgt aacctggaga gcctttcttg agccaggaat acactgaaga caaagaagtc
    17221 acattgacaa agttaagtag cggccgccgc cttctggagg cattgctgat ccttattgtc
    17281 ctgtttgccg tctggttgat ggctgcctta ctaagcttta acccttcgga ccccagctgg
    17341 tcgcaaacgg cctggcatga acctatccat aatttaggtg ggatgcccgg tgcgtggttg
    17401 gcagatacgc tgttctttat ttttggcgtg atggcttaca ccattcccgt cattattgtc
    17461 ggcggttgtt ggtttgcctg gcgtcatcag tccagcgacg aatacattga ttattttgcc
    17521 gtttcgctac gcatcattgg cgttttggcg ctcatcctta cctcctgtgg tctggcggca
    17581 atcaacgctg acgatatctg gtattttgcc tccggtggcg tcattggcag cttactaagc
    17641 actacgctac aaccactgct acacagtagc gggggaacta ttgcgctgct ctgcgtttgg
    17701 gcagcgggcc tgacgttgtt caccggttgg tcatgggtga ccattgctga aaaactcggc
    17761 ggctggattt taaacattct caccttcgcc agtaatcgta cccgtcgcga tgatacctgg
    17821 gtcgatgaag atgagtatga agacgacgaa gagtatgaag atgaaaatca cggcaaacag
    17881 catgaatcac gccgtgcccg tattcttcgc ggcgcgctag cgcgtcgtaa acggttggcg
    17941 gaaaaattca ttaatccgat ggggcggcaa acagacgctg cgttgttctc cggtaagcgg
    18001 atggatgatg acgaagagat tacctacact gcacgcggtg tggctgccga cccggacgac
    18061 gtcctatttt cgggcaatcg tgcaacgcag ccagaatatg acgaatacga tccattatta
    18121 aacagtgcgc caattaccga acctgtcgct gtagcagctg ctgctaccac ggcgacacaa
    18181 agctgggctg cgccggttga acctgtgact cagacgccgc ctgttgcctc tgttgatgtt
    18241 ccaccttcgc aacctacagt agcctggcag cctgtaccgg gtccacaaac gggagagccg
    18301 gttattgctc ctgcaccgga aggttaccca cagcagtcac aatatgcgca gcctgcagtg
    18361 caatataatg agccgctgca acaaccagta cagccgcagc agccgtatta tgcacctgca
    18421 gctgaacaac ctgcgcaaca gccgtattat gcccctgcgc cagaacaacc ggtggcaggt
    18481 aacgcctggc aagccgaaga gcagcaatcc acttttgctc cacagtctac ataccagact
    18541 gagcaaactt atcagcagcc agccgctcag gagccgttgt accaacagcc gcaacccgtt
    18601 gaacagcagc ctgttgtgga gcctgaaccc gttgtagaag agacaaaacc cgcgcgtccg
    18661 ccgctttact attttgaaga agtggaagag aagcgagccc gtgaacgtga acaacttgcg
    18721 gcctggtatc aaccgattcc agaaccggtt aaagaaccag aaccgatcaa atcttcgctg
    18781 aaagcacctt ctgttgcagc agtacctcca gtagaagccg ctgccgctgt ttctccgctg
    18841 gcatctggcg tgaaaaaagc gacactggcg acgggggctg ccgcaaccgt tgccgcgcca
    18901 gtcttcagtc tggcaaatag cggtggaccg cgtccgcagg tcaaagaggg gattggcccg
    18961 cagttgccac gaccgaaacg tatccgcgtg ccaactcgtc gtgaattggc gtcttacggt
    19021 attaagctgc cctcacagcg tgcggcggaa gaaaaagccc gtgaagccca gcgcaatcag
    19081 tatgattctg gcgatcagta taacgatgat gaaatcgatg cgatgcagca ggatgaactg
    19141 gcacgtcagt tcgcccagac acagcagcaa cgctatggcg aacagtatca acatgatgtg
    19201 cccgtaaacg cagaagatgc agatgctgcg gcagaggctg aactggctcg tcagtttgcg
    19261 caaactcaac aacaacgtta ttccggcgaa caaccggctg gggcgaatcc gttctcgctg
    19321 gatgattttg aattttcgcc aatgaaagcg ttgctggatg atggtccaca cgaaccgctg
    19381 tttacgccaa ttgttgaacc tgtacagcag ccgcaacaac cggttgcacc acagcagcag
    19441 tatcagcagc cacaacagcc ggttgcgcca cagcagcagt atcagcagcc acaacagcag
    19501 gttgcgccgc agccacaata tcagcagccg caacaaccgg ttgcgccaca gcagcaatat
    19561 cagcagccgc aacaaccagt tgcgccgcag ccgcagtatc aacagccgca acaaccggtt
    19621 gcaccgcagc cgcagtatca gcagccacaa cagccagttg cgccacagcc gcaggatacc
    19681 ctgcttcatc cgctgttgat gcgtaatggc gacagccgtc cgttgcacaa accgacgacg
    19741 ccactgcctt ctctggattt gctgacaccg ccgccgagcg aagtggagcc ggtagatacc
    19801 tttgcgcttg aacaaatggc gcgtctggtg gaagcgcgtc tggctgattt ccgtattaaa
    19861 gccgatgtcg ttaattactc tccggggcca gttatcactc gctttgaact taacctggct
    19921 cctggcgtaa aagcggcgcg catttctaac ttgtcacggg accttgcccg ttcactttcg
    19981 acggtggcgg tgcgtgtcgt tgaagttatt cctggcaaac cctatgtagg tctggagtta
    20041 ccaaataaaa aacgacaaac cgtttatctg cgcgaagttt tggataacgc caaattccgc
    20101 gataatccgt cgccattaac cgtggtgctg ggtaaagata tcgccggtga gccggtggtt
    20161 gccgatctgg cgaaaatgcc gcacttgttg gttgcgggga ctaccggttc cggtaaatct
    20221 gtcggtgtga acgcgatgat cctgagcatg ctttataaag cacagccaga agatgtgcgt
    20281 ttcatcatga tcgacccgaa aatgctggag ctttcggttt atgaaggcat tccgcacctg
    20341 ttaacggaag tcgttactga tatgaaagat gccgccaacg cgctgcgctg gtgtgttaac
    20401 gagatggagc gtcggtataa actgatgtct gcgctgggtg tgcgtaatct ggcgggttat
    20461 aacgaaaaaa ttgctgaagc cgatcgcatg atgcgtccga ttccagaccc gtactggaag
    20521 ccgggtgaca gtatggatgc ccagcatccg gtgctgaaaa aagaaccata cattgtcgtg
    20581 ttggttgacg aatttgccga cctgatgatg acggtaggta aaaaagtgga agagctgata
    20641 gctcgtctgg cgcaaaaagc ccgtgccgcg ggtatccacc tcgtactggc aactcagcgt
    20701 ccatcggttg atgttattac tggtctgatt aaagcgaata ttccgacccg tatcgccttt
    20761 accgtatcca gtaagattga ctcacgtacc attcttgatc aggctggcgc ggaatcactg
    20821 ctgggtatgg gggatatgct ctactctggg ccgaactcca cgttgccggt acgtgtccat
    20881 ggtgcttttg ttcgcgatca ggaagttcat gccgtggtgc aggactggaa agcgcgtggt
    20941 cgcccacagt atgttgatgg catcacctcc gacagcgaaa gcgaaggtgg tgcgggtggt
    21001 ttcgatggcg ctgaagaact ggatccgttg ttcgatcagg cggtgcagtt tgtcactgaa
    21061 aaacgcaaag cgtcaatttc tggcgtacag cgtcagttcc gcattggtta taaccgtgca
    21121 gcgcgtatta tcgaacagat ggaagcgcag gggattgtca gcgaacaggg gcacaacggt
    21181 aatcgtgaag tgctggcccc accgccgttt gactaattaa tgcattgccg gataaggcgc
    21241 ggtagcgtcg catccggcac tctatcaact gaaaattcag tattttcttc tttcctcaag
    21301 ctgattatta gcctggaata gagagtagag ggaactcccg atcgggagtg acgtaatttg
    21361 aggaataatg atgaaaaaaa ttgctatcac ctgtgcatta ctctcaagct tagtagcaag
    21421 cagcgtttgg gctgatgccg caagcgatct gaaaagccgc ctggataaag tcagcagctt
    21481 ccacgccagc ttcacacaaa aagtgactga cggtagcggc gcggcggtgc aggaaggtca
    21541 gggcgatctg tgggtgaaac gtccaaactt attcaactgg catatgacac aacctgatga
    21601 aagcattctg gtttctgacg gtaaaacact gtggttctat aacccgttcg ttgagcaagc
    21661 tacggcaacc tggctgaaag atgccaccgg taatacgccg tttatgctga ttgcccgcaa
    21721 ccagtccagc gactggcagc agtacaatat caaacagaat ggcgatgact ttgtcctgac
    21781 gccgaaagcc agcaatggca atctgaagca gttcaccatt aacgtgggac gtgatggcac
    21841 aatccatcag tttagcgcgg tggagcagga cgatcagcgc agcagttatc agctgaaatc
    21901 ccagcaaaat ggggctgtgg atgcagcgaa atttaccttc accccgccgc aaggcgtcac
    21961 ggtagatgat caacgtaagt agaggcacct gagtgagcaa tctgtcgctc gatttttcgg
    22021 ataatacttt tcaacctctg gccgcgcgta tgcggccaga aaatttagca cagtatatcg
    22081 gtcagcaaca tttgctggct gcggggaagc cgttgccgcg cgctatcgaa gccgggcatt
    22141 tgcattctat gatcctctgg gggccaccgg gtaccggcaa aacaaccctc gctgaagtga
    22201 ttgcccgcta tgcgaacgct gatgtggaac gtatttctgc cgtcacctct ggcgtgaaag
    22261 agattcgcga ggcgatcgag cgcgcccggc aaaaccgcaa tgcaggtcgc cgcactattc
    22321 tttttgttga cgaagttcac cgtttcaaca aaagccagca ggatgcattt ctgccacata
    22381 ttgaagacgg caccatcact tttattggcg caaccactga aaacccgtcg tttgagctta
    22441 attcggcact gctttcccgt gcccgtgttt atctgttgaa atccctgagt acagaggata
    22501 ttgagcaagt actaactcag gcgatggaag acaaaacccg tggctatggt ggtcaggata
    22561 ttgttctgcc agatgataca cgacgtgcca ttgcagaact ggtgaatggc gacgcgcgcc
    22621 gggcgttaaa tacgctggaa atgatggcgg atatggccga agtctatgat agcggtaagc
    22681 gggtcctgaa gcctgaatta ctgaccgaaa tcgccggtga acgtagcgcc cgctttgata
    22741 acaaaggcga tcgcttttac gatctaattt ccgcactgca taagtcggta cgtggtagcg
    22801 cacccgatgc ggcgctgtac tggtatgcgc gaattattac cgctggtggc gatccgttat
    22861 atgtcgcgcg tcgctgtctg gcgattgcgt ctgaagacgt cggtaatgcc gatccacggg
    22921 cgatgcaggt ggcaattgcg gcctgggatt gctttactcg cgttggcccg gcggaaggtg
    22981 aacgcgccat tgctcaggcg attgtttacc tggcctgcgc gccaaaaagc aacgctgtct
    23041 acactgcgtt taaagccgcg ctggccgatg ctcgcgaacg cccggattat gacgtgccgg
    23101 ttcatttgcg taatgcgccg acgaaattaa tgaaggaaat gggctacggg caggaatatc
    23161 gttatgctca tgatgaagca aacgcttatg ctgccggtga ggtttacttc ccgccggaaa
    23221 tagcacaaac acgctattat ttcccgacaa acaggggcct tgaaggcaag attggtgaaa
    23281 agctcgcctg gctggctgaa caggatcaaa atagccccat aaaacgctac cgttaatgtt
    23341 atcgttgcgg taatgttgtt actgtatccc tgtggtcgca ggctgtggcc acatctccca
    23401 tttaattcga taagcacagg ataagcatgc tcgatcccaa tctgctgcgt aatgagccag
    23461 acgcagtcgc tgaaaaactg gcacgccggg gctttaagct ggatgtagat aagctgggcg
    23521 ctcttgaaga gcgtcgtaaa gtattgcagg tcaaaacgga aaacctgcaa gcggagcgta
    23581 actcccgatc gaaatccatt ggccaggcga aagcgcgcgg ggaagatatc gagcctttac
    23641 gtctggaagt gaacaaactg ggcgaagagc tggatgcagc aaaagccgag ctggatgctt
    23701 tacaggctga aattcgcgat atcgcgctga ccatccctaa cctgcctgca gatgaagtgc
    23761 cggtaggtaa agacgaaaat gacaacgttg aagtcagccg ctggggtacc ccgcgtgagt
    23821 ttgactttga agttcgtgac cacgtgacgc tgggtgaaat gcactctggc ctcgactttg
    23881 cagctgcagt taagctgact ggttcccgct ttgtggtaat gaaagggcag attgctcgca
    23941 tgcaccgcgc actgtcgcag tttatgctgg atctgcatac cgaacagcat ggctacagtg
    24001 agaactatgt tccgtacctg gttaaccagg acacgctgta cggtacgggt cagctgccga
    24061 aatttgctgg cgatctgttc catactcgtc cgctggaaga agaagcagac accagtaact
    24121 atgcgctgat cccaacggca gaagttccgc tgactaacct ggtgcgcggt gaaatcatcg
    24181 atgaagatga tctgccaatt aagatgaccg cccacacccc atgcttccgt tctgaagccg
    24241 gttcatatgg tcgtgacacc cgtggtctga tccgtatgca ccagttcgac aaagttgaaa
    24301 tggtgcagat cgtgcgccca gaagactcaa tggcggcgct ggaagagatg actggtcatg
    24361 cagaaaaagt cctgcagttg ctgggcctgc cgtaccgtaa aatcatcctt tgcactggcg
    24421 acatgggctt tggcgcttgc aaaacttacg acctggaagt atggatcccg gcacagaaca
    24481 cctaccgtga gatctcttcc tgctccaacg tttgggattt ccaggcacgt cgtatgcagg
    24541 cacgttgccg cagcaagtcg gacaagaaaa cccgtctggt tcataccctg aacggttctg
    24601 gtctggctgt tggtcgtacg ctggttgcag taatggaaaa ctatcagcag gctgatggtc
    24661 gtattgaagt accagaagtt ctgcgtccgt atatgaacgg actggaatat attggctaat
    24721 acccaatttt tctgaatcta aaaagcgcct ttagggcgct ttttttgtct ccctttgata
    24781 ccgaacaata attactcctc acttacacgt aatactactt tcgagtgaaa atctacctat
    24841 ctctttgatt ttcaaattat tctatgtata caagcctata tagcgagctg ctatagaaat
    24901 aattacacaa tacggtttgt tactggaatc aatcgtgatc aagcttgagt gagccattat
    24961 gaaaacgaaa atccctgatg cggtattggc tgctgaggag agtcgccgtg gtttggtaaa
    25021 aacgacagcg atcggcggcc tggcaatggc cagcagcgca ttaacattac cttttagtcg
    25081 gattgcgcac gctgtcgata gcgccattcc aacaaaatca gacgaaaagg ttatctggag
    25141 cgcctgtaca gttaactgtg gtagtcgctg cccgctacgt atgcacgtcg tggacggtga
    25201 aatcaaatat gtcgaaacga caataccggc gatgacaatt acgacggcct acactaggtt
    25261 cgcgcctgcc tgcgtgggcg ttccatgcgt caccgtgtct acaatccgga ccgcctgaaa
    25321 tatccgatga aacgagtcgg ggcgcgcggt gaaggcaaat tcgagcgcat tagctgggaa
    25381 gaagcctacg acatcatcgc gaccaatatg cagcgcctga tcaaagagta cggcaacgag
    25441 tctatctatc tgaactatgg caccggtacg ctgggcggca ccatgacccg ctcctggccg
    25501 ccgggaaata ccctggtcgc gcgcctgatg aactgctgcg gcggttatct taaccattac
    25561 ggtgactact cttccgcgca aattgcggaa ggtttgaacc atacctacgg cggctgggca
    25621 gatggtaaca gcccgtcgga tatcgaaaac agtaaattgg tagtgctgtt tggtaataac
    25681 ccaggcgaaa cgcgaatgag tggcggtggg gtgacttact atcttgaaca ggcacgccag
    25741 aaatctaatg ctcgcatgat catcatcgat ccgcgctata ccgacaccgg tgccgggcgc
    25801 gaagatgagt ggatccctat tcgtccagga acagatgccg cactggttaa cggtctggcg
    25861 tacgtcatga tcactaaaaa cctggtggat caggcattcc tcgataaata ttgcgttggc
    25921 tatgacgaga aaaccctgcc cgccagtgcg ccgaaaaatg gtcattataa agcttatatt
    25981 ctgggtgaag ggccagatgg cgtggctaaa acaccggaat gggcctcgca aatcaccggt
    26041 gttccggcag acaaaatcat caaactggct cgtgaaatcg gcagtaccaa accggcgttt
    26101 atcagccagg gatggggccc gcagcgtcac gctaacggtg aaatcgcaac ccgtgctatc
    26161 tcgatgctgg cgattctgac cggtaacgtt ggtattaatg gagggaacag cggcgcgcgt
    26221 gaaggttcat acagcttacc gtttgtccgt atgccgacct tggaaaaccc gatccagacc
    26281 agcatttcga tgtttatgtg gaccgatgcc attgaacgtg gcccggaaat gacggcgctg
    26341 cgtgatggtg tgcgcgggaa agataagctg gatgtgccga tcaaaatgat ctggaactat
    26401 gccggtaact gcctgattaa ccagcattct gaaatcaacc gtacccatga aatcctccag
    26461 gatgataaga agtgcgagct gattgtggtt atcgactgcc acatgacctc atcggcgaaa
    26521 tatgctgaca tcctgctgcc tgactgcacc gcttccgaac agatggactt tgcactggat
    26581 gcatcctgcg ggaatatgtc ttacgtgatt ttcaacgatc aggtgattaa accgcgcttt
    26641 gaatgtaaga ccatctatga aatgaccagc gaactggcaa aacgtcttgg cgttgagcaa
    26701 cagtttactg aaggccgtac ccaggaagag tggatgcggc atctgtatgc ccagtcgcgg
    26761 gaagcgattc ctgaactgcc aacgtttgaa gagttccgca agcaggggat ctttaaaaag
    26821 cgcgacccac aagggcatca cgttgcttat aaagccttcc gtgaagatcc gcaggcaaac
    26881 ccactgacta cgccatcggg caaaattgag atttattcgc aggcgctggc tgacattgcc
    26941 gctacctggg aattgccaga aggcgatgtg atcgatccac tgccgatcta cacgccgggc
    27001 tttgaaagtt atcaggatcc gctgaacaaa cagtatccgc tgcagcttac aggtttccac
    27061 tataaatctc gcgttcactc aacttacggc aacgttgatg tgctgaaagc ggcttgccgt
    27121 caggaaatgt ggatcaaccc gcttgatgcc caaaaacgcg gtatccacaa cggcgataaa
    27181 gtcaggatct ttaacgatcg tggtgaggtt catattgagg cgaaagtgac gccacgaatg
    27241 atgccgggcg tggtcgcact gggtgaaggc gcctggtatg acccggatgc aaaacgtgtc
    27301 gataagggcg gttgtatcaa cgtactgacc actcaacgtc cgtctcctct cgctaagggg
    27361 aatccgtcac atacaaacct tgttcaggtt gaaaaggtgt aaggagtaac cgatgacaac
    27421 ccagtatgga ttttttattg attccagccg ttgcaccggt tgcaaaacct gcgagctggc
    27481 ttgtaaagac tacaaagatt tgacgccaga agtcagtttc cgccgcattt atgaatatgc
    27541 tggcggcgac tggcaggaag ataacggtgt ctggcaccag aacgtgtttg cctactatct
    27601 gtcgatttcg tgcaaccact gcgaagatcc ggcttgtact aaagtctgcc cgagcggtgc
    27661 gatgcataaa cgcgaagatg gttttgttgt ggtcgatgaa gatgtatgca ttggctgccg
    27721 ttactgtcat atggcttgcc cgtatggcgc accgcaatat aacgaaacga aaggccatat
    27781 gaccaaatgc gatggttgtt atgaccgtgt tgcggagggt aaaaagccga tctgtgttga
    27841 atcttgcccg ctgcgggcgc tggatttcgg ccctatcgac gagctgcgta aaaaacatgg
    27901 cgatctggcg gccgttgcgc cgttgccgcg agctcacttt accaaaccga atattgtgat
    27961 caaacccaat gccaatagcc gcccgaccgg ggataccact ggctatctgg caaacccgaa
    28021 ggaggtgtga gatgggaagt ggatggcatg aatggccgct gatgatcttc acggtcttcg
    28081 ggcaatgtgt ggcgggtggt tttatcgttc tggctttggc gctgctcaaa ggcgacctgc
    28141 gagcagaagc ccagcagcgt gttatcgcct gcatgtttgg cttatgggta ctgatgggca
    28201 ttggctttat cgcctctatg ctccatcttg gttcaccgat gcgcgccttt aactcgctca
    28261 accgggtagg ggcttcagca ctcagtaacg aaatcgccag cggttcgatc ttttttgctg
    28321 taggcggcat cggctggttg ctggcaatgc tgaaaaagct gtcaccggca ttgcgtacgc
    28381 tatggctggt ggtgacgatg gttcttggcg tcgtctttgt ctggatgatg gtgcgtgtgt
    28441 ataacagcat tgataccgtt ccgacctggt acagcatctg gacgccgatg ggctgggcga
    28501 tgcgtctgct gccagccatt tctgtactgg cactggtggt gagtggcgtg atgtcagtga
    28561 tgcagggcac agagctggca actattcata gttctgtgca gcaggccgca gcgctggtgc
    28621 cggactatgg tgcgctgatg tcctggcgga tcgtgctttt ggccgttgcc ctgtgcttgt
    28681 ggattgcacc acagctaaaa ggttatcagc ctgcggttcc gctactatcc gtctcattca
    28741 ttctgcttct ggcaggggaa ttaatcggtc gcggcgtatt ctatggtttg catatgaccg
    28801 tggggatggc cgtcgcaagc taatcagtaa caaccggggt ttcggccccg gttctctttt
    28861 atttctgctt cgttaacgtg tcataactgg tcatcaaatt acgataatca ggaatatggt
    28921 tagagaacaa cgtcgccagt ccttcaatat cattacgcca gtcacggtgc agctcgcaag
    28981 ccacgccgaa ccatgtcatc aattgcgcac cagcttgcga catgcgatcc cacgccgaat
    29041 gccgggtaat ttcattaaag gtccctgatg catcagtcac gacaaagaca tcaaaccctt
    29101 cttcaatggc tgaaagcgcc gggaatgcca cgcaaacttc ggttaccaca ccggcaataa
    29161 ttaactgttt tttacctgtc gctttgactg cttttacaaa atcttcgtta tcccaggcgt
    29221 taatattgcc cgggcgagca atgtaaggtg catcggggaa ttgtgctttc agttctagaa
    29281 ctaatgggcc gttaggaccg gtttcaaaac tggtggtaag aatagttggt aagttgaaat
    29341 acttagccag gtcacccagc gccagcacgt tatttttaaa cttatcgggt tcgatatccc
    29401 gtacaaggga aagtaaacca gcctggtgat caacaagcaa aacggcagca tcatttttat
    29461 caagacgaac atacggtttg gtcatcctct tctcctttcg agagtcagtg gtacgtctga
    29521 aatcggtcag acgcgatgca ttgctctgaa agcatagacg ggaaatatga gtttgctgtg
    29581 accatgaaat ttttcgactg aacgaagcgt atgaaatttg tgttagatca ataaaaacaa
    29641 tcagatgaga ttaatcatct cttcagcatt agcgttatga atgagtaata taaatcgata
    29701 ataccgccgc tgcgatatgc gcgtattttc acccattgac aatgtttttg gcggtggcat
    29761 gatgcgcatg aaatttgaac ttcctcacgg ttttaattca tgtccacgta tacccggcct
    29821 gtcatgcttt tgctgtctgg cctgcttttg ttgactctgg cgatcgcggt gttaaataca
    29881 ctcgtgccgc tttggctcgc ccaggaacac atgtccacat ggcaggtagg cgttgtcagc
    29941 tcatcctatt ttaccggcaa ccttgtcggt acattgctga cagggtatgt cattaaacgc
    30001 attggcttta accgcagcta ttatctgtcc tccttcattt ttgctgctgg ctgtgccggc
    30061 cttggcctga tgattggttt ctggagctgg ttggcgtggc gttttgtcgc gggcgtcggc
    30121 tgtgccatga tttgggtggt tgttgagagc gcgctgatgt gcagtgggac gtcacgtaac
    30181 cgtgggcgtt tgcttgctgc gtatatgatg gtttattacg tgggaacgtt tttaggccag
    30241 ttactggtca gcaaagtctc aaccgagctg atgagcgtat tgccgtgggt tacaggtttg
    30301 acattggcag ggatcttacc gctgttgttt acgcgtgtcc tgaatcagca ggctgaaaac
    30361 catgattcga cgtcaattac ggcaatgcta aaactccgtc aggcgcggct tggcgtgaat
    30421 ggctgcatta tctcaggaat cgttctggga tctctatatg gcctgatgcc tctgtacctc
    30481 aatcacaaag gggtgagcaa tgccagtatt ggtttctgga tggcggtact ggtcagtgcg
    30541 ggtatcctcg gacaatggcc gattggacgt ctggcggata agtttggtcg actgctggtt
    30601 ttgcgtgttc aggtctttgt cgtcattctc ggcagtattg cgatgcttag ccaggcggcg
    30661 atggccccag cgttattcat cctcggtgcc gctggcttta cgctatatcc ggtggcgatg
    30721 gcctgggctt gtgagaaagt tgaacatcat caactggtgg cgatgaacca agccttactg
    30781 ttgagctata
//
LOCUS       DAJTRC010000043        30105 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.043, whole genome shotgun sequence.
ACCESSION   DAJTRC010000043 DAJTRC010000000
VERSION     DAJTRC010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30105)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30105)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30105
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.043"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..676)
                     /gene="wcaD"
                     /locus_tag="OO357_002077"
     CDS             complement(<1..676)
                     /gene="wcaD"
                     /locus_tag="OO357_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative colanic acid polymerase WcaD"
                     /protein_id="HCR5887028.1"
                     /translation="MSTSIRICSYLLLPLIYLLVNVKIAQLGESFPITIVTFLPVLLL
                     LFLERISIKKLMIALGIGAGLTAFNYLFGQSLDAGKYVTSTMLFVYIVIIIGMVWSIR
                     FKTISPHNHRKILRFFYLVVGLVVALAAVEMAQIILTGGSSIMESISKYLIYSNSYVL
                     NFIKFGGKRTTALYFEPAFFALALISIWLSIKQFGIKTPKTDALILAGIILSGSFSGV
                     MTFILFY"
     gene            complement(651..1868)
                     /gene="wcaC"
                     /locus_tag="OO357_002078"
     CDS             complement(651..1868)
                     /gene="wcaC"
                     /locus_tag="OO357_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaC"
                     /protein_id="HCR5887029.1"
                     /translation="MNILQFNVRLAEGGAAGVALDLHQRALQQGLASHFVYGYGKGGK
                     ESVSHQNYPQVIKHTPRMTAMANIALFRLFNRDLFGNFNELYRTITRTPGPVVLHFHV
                     LHSYWLNLNSVVRFCEKVKNHKPDVTLVWTLHDHWSVTGRCAFTDGCEGWKTGCQKCP
                     TLNNYPPVKIDRAHQLVAGKRQLFREMLALGCQFISPSQHVADAFNSLYGPGRCRIIN
                     NGIDMATEAILADLPPVRETQGKPKIAVVAHDLRYDGKTNQQLVREMMALGDKIELHT
                     FGKFSPFTAGNVVNHGFETDKRKLMSALNQMDALVFSSRVDNYPLILCEALSIGVPVI
                     ATHSDAAREVLQKSGGKTVSEEEVLQLVQLSKPEIAQAIFGTTLAEFSQRSRAAYSGQ
                     QMLEEYVNFYQNL"
     gene            complement(1865..2353)
                     /gene="wcaB"
                     /locus_tag="OO357_002079"
     CDS             complement(1865..2353)
                     /gene="wcaB"
                     /locus_tag="OO357_002079"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis acetyltransferase
                     WcaB"
                     /protein_id="HCR5887030.1"
                     /translation="MLEDLRANSWSLRPCCMVLAYRVAHFCSVWRKKNVLNNLWAAPL
                     LVLYRIITECFFGYEIQAAATIGRRFTIHHGYAVVINKNVVAGDDFTIRHGVTIGNRG
                     ADNMACPHIGNGVELGANVIILGDITLGNNVTVGAGSVVLDSVPDNALVVGEKARVKV
                     IK"
     gene            complement(2356..3195)
                     /gene="wcaA"
                     /locus_tag="OO357_002080"
                     /pseudo
     CDS             complement(2356..3195)
                     /gene="wcaA"
                     /locus_tag="OO357_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310891.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid biosynthesis glycosyltransferase
                     WcaA"
     gene            complement(3288..5450)
                     /gene="wzc"
                     /locus_tag="OO357_002081"
     CDS             complement(3288..5450)
                     /gene="wzc"
                     /locus_tag="OO357_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase Wzc"
                     /protein_id="HCR5887031.1"
                     /translation="MTEKVKQHAAPVTGSDEIDIGRLVGTVIEARWWVIGITVVFALC
                     AVVYTFFATPIYSADALVQIEQNSGNSLVQDIGSALANKPPASDAEIQLIRSRLVLGK
                     TVDDLDLDIAVSKNTFPIFGAGWDRLMGRQNETVKVTTFNRPKEMADQVFTLNVLDDK
                     NYTLSSDGGFSARGQAGQMLKKEGVTLMVEAIHARPGSEFTVTKYSTLGMINQLQNSL
                     TVTENGKDAGVLSLTYTGEDREQIRDILNSIARNYQEQNIERKSAEASKSLAFLAQQL
                     PEVRSRLDVAENKLNAFRQDKDSVDLPLEAKAVLDSMVNIDAQLNELTFKEAEISKLY
                     TKVHPAYRTLLEKRQALEEEKAKLNGRVTAMPKNQQEIVRLTRDVESGQQVYMQLLNK
                     EQELKITEASTVGDVRIIDPAITQPGVLKPKKGLIILGAIILGLMLSIVGVLLRSLFN
                     RGIESPQVLEEHGISVYASIPLSEWQKARDSVKTIKGVKRYKQSQLLAVGNPTDLAIE
                     AIRSLRTSLHFAMMQAQNNVLMMTGVSPSIGKTFVCANLAAVISQTNKRVLLIDCDMR
                     KGYTHELLGTNNVNGLSEILIGQGDITTAAKPTSIAKFDLIPRGQVPPNPSELLMSER
                     FAELVNWASKNYDLVLIDTPPILAVTDAAIVGRHVGTTLMVARYAVNTLKEVETSLSR
                     FEQNGIPVKGVILNSIFRRASAYQDYGYYEYEYKSDAK"
     gene            complement(5453..5896)
                     /gene="wzb"
                     /locus_tag="OO357_002082"
     CDS             complement(5453..5896)
                     /gene="wzb"
                     /locus_tag="OO357_002082"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase Wzb"
                     /protein_id="HCR5887032.1"
                     /translation="MFNNILVVCVGNICRSPMAERLLQRYHPELKVESAGLGALVGKG
                     ADPTAISVAAEHQLSLEGHCARQISRRLCRNYDLILTMDKRHIERLCEMAPEMRGKVM
                     LFGHWDNECEIPDPYHKSRETFAAVYTLLERSARQWAQALNAEQV"
     gene            complement(5902..7041)
                     /gene="wza"
                     /locus_tag="OO357_002083"
     CDS             complement(5902..7041)
                     /gene="wza"
                     /locus_tag="OO357_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein Wza"
                     /protein_id="HCR5887033.1"
                     /translation="MMKSKMKLMPLLVSVTLISGCTVLPGSNMSTMGKDVIKQQDADF
                     DLDKMVNVYPLTPRLIDQLRPRPNVARPNMTLESEIANYQYRVGPGDVLNVTVWDHPE
                     LTTPAGQYRSSSDTGNWVQPDGTMFYPYIGKVHVVGKTLAEIRSDITGRLATYIADPQ
                     VDVNIAAFRSQKAYISGQVNKSGQQAITNVPLTILDAINAAGGLTDTADWRNVVLTHN
                     GREERISLQALMQNGDLNQNRLLYPGDILYVPRNDDLKVFVMGEVKKQSTLKMDFSGM
                     TLTEALANAEGIDMTTSNASGIFVIRPLKGEGGRNGKIANIYQLDMSDATSLVMATEF
                     RLQPYDVVYVTTAPVSRWNRLINQLLPTISGVRYMTDTASDIHNW"
     gene            7350..7499
                     /locus_tag="OO357_002084"
     CDS             7350..7499
                     /locus_tag="OO357_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017458496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887034.1"
                     /translation="MVIEYWLANNAGGSVTANGIAGKVTIRQLAIFKNYIKYHSIWFL
                     GVSLG"
     gene            7700..9283
                     /locus_tag="OO357_002085"
     CDS             7700..9283
                     /locus_tag="OO357_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HCR5887035.1"
                     /translation="MEWIADPSIWAGLITLIVIELVLGIDNLVFIAILAEKLPPKQRD
                     RARVTGLLLAMLMRLLLLASISWLVTLTQPLFSFRSFTFSARDLIMLFGGFFLLFKAT
                     MELNERLEGKDSNNPTQRKGAKFWGVVTQIVVLDAIFSLDSVITAVGMVDHLLVMMAA
                     VVIAISLMLMASKPLTQFVNSHPTIVILCLSFLLMIGFSLVAEGFGFVIPKGYLYAAI
                     GFSVMIEALNQLAIFNRRRFLSANQTLRQRTTEAVMRLLSGQKEDAELDAKTASMLVD
                     HGNQQIFNPQERRMIERVLNLNQRTVSSIMTSRHDIEHIDLNAPEEEIRQLLERNQHT
                     RLVVTDGDDAEDLLGVVHVIDLLQQSLRGEPLNLRVLIRQPLVFPETLPLLPALEQFR
                     NARTHFAFVVDEFGSVEGIVTLSDVTETIAGNLPNEVEEIDARHDIQKNADGSWTANG
                     HMPLEDLVQYVPLPLDEKREYHTIAGLLMEYLQRIPKPGEEVQVGDYLLKTLQVESHR
                     VQKVQIIPLRKDGEMEYEV"
     gene            complement(9735..11588)
                     /gene="asmA"
                     /locus_tag="OO357_002086"
     CDS             complement(9735..11588)
                     /gene="asmA"
                     /locus_tag="OO357_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane assembly protein AsmA"
                     /protein_id="HCR5887036.1"
                     /translation="MRRFLTTLMILLVVLVAGLSALVLLVNPNDFRDYMVKQVAARSG
                     YQLQLDGPLRWHVWPQLSILSGRMSLTAQGASQPLVRADNMRLDVALLPLLSHQLSVK
                     QVMLKGAVIQLTPQTEAVRSEDAPVAPRDNTLPDLSDDRGWSFDISSLKVADSVLVFQ
                     HEDDEQVTIRNIRLQMEQDPQHRGSFEFSGRVNRDQRDLTISLNGTVDASDYPHDLTA
                     AIEQINWQLQGADLPKQGIQGQGSFQSQWQESHKRLSFNQISLTANDSTLSGQAQVTL
                     TEKPEWQLRLQFPQLNLDNLIPLNETANGENGAAQQGQSQSTLPRPVISSRIDEPAYQ
                     GLQGFTADILLQASNVRWRGMNFTDVATQMTNKSGLLEITQLQGKLNGGKVSLPGTLD
                     ATSINPRINFQPRLENVEIGTILKAFNYPISLTGKMSLAGDFSGADIDADAFRHNWQG
                     QAHVEMTDTRMEGMNFQQMIQQAVERNGGDVKAAENFDNVTRLDRFTTDLTLKDGVVT
                     LNDMQGQSPVLALTGEGMLNLADQTCDTQFDIRVVGGWNGESKLIDFLKETPVPLRVY
                     GNWQQLNYSLQVDQLLRKHLQDEAKRRLNDWAERNKDSRNGKDVKKLLEKM"
     gene            complement(11610..12191)
                     /gene="dcd"
                     /locus_tag="OO357_002087"
     CDS             complement(11610..12191)
                     /gene="dcd"
                     /locus_tag="OO357_002087"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004942378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HCR5887037.1"
                     /translation="MRLCDRDIEAWLDEGRLSINPRPPVERINGATVDVRLGNKFRTF
                     RGHTAAFIDLSGPKDEVSAALDRVMSDEIVLDEGEAFYLHPGELALAVTLESVTLPAD
                     LVGWLDGRSSLARLGLMVHVTAHRIDPGWSGCIVLEFYNSGKLPLALRPGMLIGALSF
                     EPLSGPAARPYNRREDAKYRNQQGAVASRIDKD"
     gene            complement(12283..12924)
                     /gene="udk"
                     /locus_tag="OO357_002088"
     CDS             complement(12283..12924)
                     /gene="udk"
                     /locus_tag="OO357_002088"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HCR5887038.1"
                     /translation="MTDQSHQCVIIGIAGASASGKSLIASTLYRELREQVGDEHIGVI
                     PEDCYYKDQSHLSMEERVKTNYDHPSAMDHSLLLEHLQALKRGSAIDLPVYSYVEHTR
                     MKETVTVEPKKVIILEGILLLTDARLRDELNFSIFVDTPLDICLMRRIKRDVNERGRS
                     MDSVMAQYQKTVRPMFLQFIEPSKQYADIIVPRGGKNRIAIDILKAKISQFFE"
     gene            13242..13484
                     /locus_tag="OO357_002089"
                     /pseudo
     CDS             13242..13484
                     /locus_tag="OO357_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MASE1 domain-containing protein"
     gene            13483..14424
                     /gene="mdtA"
                     /locus_tag="OO357_002090"
                     /pseudo
     CDS             13483..14424
                     /gene="mdtA"
                     /locus_tag="OO357_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416578.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter subunit MdtA"
     gene            14424..17546
                     /gene="mdtB"
                     /locus_tag="OO357_002091"
                     /pseudo
     CDS             14424..17546
                     /gene="mdtB"
                     /locus_tag="OO357_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310910.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtB"
     gene            17547..20612
                     /gene="mdtC"
                     /locus_tag="OO357_002092"
     CDS             17547..20612
                     /gene="mdtC"
                     /locus_tag="OO357_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux RND transporter permease
                     subunit MdtC"
                     /protein_id="HCR5887039.1"
                     /translation="MKFFALFIYRPVATILLSVAITLCGILGFRMLPVAPLPQVDFPV
                     IMVSASLPGASPETMASSVATPLERSLGRIAGVSEMTSSSSLGSTRIILQFDFDRDIN
                     GAARDVQAAINAAQSLLPSGMPSRPTYRKANPSDAPIMILTLTSDTYSQGELYDFAST
                     QLAPTISQIDGVGDVDVGGSSLPAVRVGLTPQALFNQGVSLDDVRTAISNANVRKPQG
                     ALEDGTHRWQIQTNDELKTAAEYQPLIIHYNNGGAVRLGDVATVTDSVQDVRNAGMTN
                     AKPAILLMIRKLPEANIIQTVDSIRAKLPELQETIPAAIDLQIAQDRSPTIRASLEEV
                     EQTLIISVALVILVVFLFLRSGRATIIPAVAVPVSLIGTFAAMYLCGFSLNNLSLMAL
                     TIATGFVVDDAIVVLENIARHLEAGMKPLQAALQGTREVGFTVLSMSLSLVAVFLPLL
                     LMGGLPGRLLREFAVTLSVAIGISLLVSLTLTPMMCGWMLKASKPREQKRLRGFGRML
                     VALQQGYGKSLKWVLNHTRLVGVVLLGTIALNISIPKTFFPEQDTGVLMGGIQADQSI
                     SFQAMRGKLQDFMKIIRDDPAVDNVTGFTGGSRVNSGMMFITLKPRDERSETAQQIID
                     RLRVKLAKEPGANLFLMAVQDIRVGGRQSNASYQYTLLSDDLAALREWEPKIRKKLAT
                     LPELADVNSDQQDNGAEMNLVYDRDTMARLGIDVQAANSLLNNAFGQRQISTIYQPMN
                     QYKVVMEVDPRYTQDISALEKMFVINNEGKAIPLSYFAKWQPANAPLSVNHQGLSAAS
                     TISFNLPTGKSLSDASAAIDRAMTQLGVPSTVRGSFAGTAQVFQETMNSQVILIIAAI
                     ATVYIVLGILYESYVHPLTILSTLPSAGVGALLALELFNAPFSLIALIGIMLLIGIVK
                     KNAIMMVDFALEAQRHGNLTPQEAIFQACLLRFRPIMMTTLAALFGALPLVLSGGDGS
                     ELRQPLEITIVGGLVMSQLLTLYTTPVVYLFFDRLRLRFSRKPKQTVTE"
     gene            20613..22028
                     /locus_tag="OO357_002093"
     CDS             20613..22028
                     /locus_tag="OO357_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5887040.1"
                     /translation="MTDLPDSTRWQLWIVAFGFFMQSLDTTIVNTALPSMAQSLGESP
                     LHMHMVIVSYVLTVAVMLPASGWLADKVGVRNIFFTAIVLFTLGSLFCALSGTLNELL
                     LARALQGVGGAMMVPVGRLTVMKIVPREQYMAAMTFVTLPGQVGPLLGPALGGLLVEY
                     ASWHWIFLINIPVGIIGAIATLMLMPNYTMQTRRFDLSGFLLLAVGMAVLTLALDGSK
                     GTGLSPLAIAGLVAVGVVALVLYLLHARNNNRALFSLKLFRTRTFSLGLAGSFAGRIG
                     SGMLPFMTPVFLQIGLGFSPFHAGLMMIPMVLGSMGMKRIVVQVVNRFGYRRVLVATT
                     LGLSLVTLLFMTTALLGWYYVLPFVLFLQGMVNSTRFSSMNTLTLKDLPDNLASSGNS
                     LLSMIMQLSMSIGVTIAGLLLGLFGSQHISVDSGTTQTVFMYTWLSMAFIIALPAFIF
                     ARVPNDTHQNVAISRRKRSAQ"
     gene            22025..23428
                     /gene="baeS"
                     /locus_tag="OO357_002094"
     CDS             22025..23428
                     /gene="baeS"
                     /locus_tag="OO357_002094"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     BaeS"
                     /protein_id="HCR5887041.1"
                     /translation="MKFWRPGITGKLFLAIFATCIVLLISMHWAVRISFERGFIDYIK
                     HGNEQRLQLLSDALGEQYAQHGNWRFLRNNDRFVFQILRSFEHDNSEDKPGPGMPPHG
                     WRTQFWVVDQNNKVLVGPRAPIPPDGTRRPILVNGAEVGAVIASPVERLTRNTDINFD
                     KQQRQTSWLIVALATLLAALATFLLARGLLAPVKRLVDGTHKLAAGDFTTRVTPTSED
                     ELGKLAQDFNQLASTLEKNQQMRRDFMADISHELRTPLAVLRGELEAIQDGVRKFTPE
                     TVASLQAEVGTLTKLVDDLHQLSMSDEGALAYQKAPVDLIPLLEVAGGAFRERFASRG
                     LKLQFSLPDSITVFGDRDRLMQLFNNLLENSLRYTDSGGSLKISAEQHDKTVRLTFAD
                     SAPGVSDDQLQKLFERFYRTEGSRNRASGGSGLGLAICLNIVEAHNGRIIAAHSPFGG
                     VSITVELPLERDLQREV"
     gene            23425..24147
                     /gene="baeR"
                     /locus_tag="OO357_002095"
     CDS             23425..24147
                     /gene="baeR"
                     /locus_tag="OO357_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BaeR"
                     /protein_id="HCR5887042.1"
                     /translation="MTELPIDENTPRILIVEDEPKLGQLLIDYLRAASYAPTLISHGD
                     QVLAYVRQTPPDLILLDLMLPGTDGLTLCREIRRFSDVPIVMVTAKIEEIDRLLGLEI
                     GADDYICKPYSPREVVARVKTILRRCKPQRELQQQDAESPLIIDEGRFQASWRGKMLD
                     LTPAEFRLLKTLSHEPGKVFSREQLLNHLYDDYRVVTDRTIDSHIKNLRRKLESLDAE
                     QSFIRAVYAVGYRWEADACRIV"
     gene            24338..24670
                     /locus_tag="OO357_002096"
     CDS             24338..24670
                     /locus_tag="OO357_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HCR5887043.1"
                     /translation="MAGWFELSKSSDNQFRFVLKAGNGETILTSELYTSKASAEKGIA
                     SVRSNSPQEERYEKKTASNGKFYFNLKAANHQIIGSSQMYATAQSRETGIASVKANGT
                     SQTVKDNT"
     gene            24817..26178
                     /gene="yegQ"
                     /locus_tag="OO357_002097"
     CDS             24817..26178
                     /gene="yegQ"
                     /locus_tag="OO357_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707973.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-hydroxyuridine modification protein YegQ"
                     /protein_id="HCR5887044.1"
                     /translation="MFKPELLSPAGTLKNMRYAFAYGADAVYAGQPRYSLRVRNNEFN
                     HENLQLGINEAHALGKKFYVVVNIAPHNAKLKTFIRDLKPVVEMGPDALIMSDPGLIM
                     LVREHFPEMPIHLSVQANAVNWATVKFWQQMGLTRVILSRELSLEEIEEIHNQVPDME
                     IEIFVHGALCMAYSGRCLLSGYINKRDPNQGTCTNACRWEYNVQEGKEDDVGNIVHKY
                     EPIPVQNVEPTLGIGAPTDKVFMIEEAQRPGEYMTAFEDEHGTYIMNSKDLRAIAHVE
                     RLTKMGVHSLKIEGRTKSFYYCARTAQVYRKAIDDAAAGKPFDTSLLETLEGLAHRGY
                     TEGFLRRHTHDDYQNYEYGYSVSDRQQFVGEFTGERKGDLAAVAVKNKFSVGDSLELM
                     TPQGNINFTLEHMENAKGEAMPVAPGDGYTVWLPVPQDLELNYALLMRNFSGETTRNP
                     HGK"
     gene            complement(26520..26837)
                     /locus_tag="OO357_002098"
     CDS             complement(26520..26837)
                     /locus_tag="OO357_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887045.1"
                     /translation="MKKIAAISLISIFLISGCAVHNDETSIGKFGLAYKSNIQRKLDN
                     QYYTEAEASLARGRISGAENIVKNDAAHFCVTQGKKMQIVDLKTEGAGLHGVARLTFK
                     CGE"
     gene            27243..28142
                     /gene="yegS"
                     /locus_tag="OO357_002099"
     CDS             27243..28142
                     /gene="yegS"
                     /locus_tag="OO357_002099"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid kinase YegS"
                     /protein_id="HCR5887046.1"
                     /translation="MAEFPASLLILNGKSTDNLPLREAIMLLREEGMTIHVRVTWEKG
                     DAARFVEEARKLGVATVIAGGGDGTINEVSTALIQCEGDDIPALGILPLGTANDFATS
                     VGIPEALDKALKLAIAGNAIAIDMAQVNKQTCFINMATGGFGTRITTETPEKLKAALG
                     GVSYIIHGLMRMDTLQPDRCEIRGENFHWQGDALVIGIGNGRQAGGGQQLCPNALIND
                     GLLQLRIFTGDEILPALVSTLKSDEDNPNIIEGASSWFDIQAPHEITFNLDGEPLSGQ
                     NFHIEILPAALRCRLPPDCPLLR"
     gene            complement(28224..28997)
                     /locus_tag="OO357_002100"
     CDS             complement(28224..28997)
                     /locus_tag="OO357_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5887047.1"
                     /translation="MNSFERRNKIIQLVNEQGTVLVQDLAGVFAASEATIRADLRFLE
                     QKGVVTRFHGGAAKIMSGNSETETQEVGFKERFQLASAPKNRIAQAAVKMIHEGMTVI
                     LDSGSTTMLIAEGLMTAKNITVITNSLPAAFALSENKDITLVVCGGTVRHKTRSMHGS
                     IAERSLQDINADLMFVGADGIDAVNGITTFNEGYSISGAMVTAANKVIAVLDSSKFNR
                     RGFNQVLPIEKIDIIVTDDAVSEVDKLALQKTRVKLITV"
     gene            complement(29103..>30105)
                     /gene="gatD"
                     /locus_tag="OO357_002101"
     CDS             complement(29103..>30105)
                     /gene="gatD"
                     /locus_tag="OO357_002101"
                     /EC_number="1.1.1.251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="galactitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5887048.1"
                     /translation="VAKSVIPEIKHQDEVRVKIASSGLCGSDLPRIFKNGAHYYPITL
                     GHEFSGYIDAVGSGVDDLHPGDAVACVPLLPCFTCPECLKGFYSQCAKYDFIGSRRDG
                     GFAEYIVVKRKNVFALPTDMPIEDGAFIEPITVGLHAFHLAQGCENKNVIIIGAGTIG
                     LLAIQCAVALGAKSVTAIDISSEKLALAKSFGAMQTFNSSEMSAPQMQSVLRELRFNQ
                     LILETAGVPQTVELAVEIAGPHAQLALVGTLHQDLHLTSATFGKILRKELTVIGSWMN
                     YSSPWPGQEWETASRLLTERKLSLEPLIAHRGSFESFTQAVRDIARNAMQGKVLLIP"
BASE COUNT         6945 a         8000 c         7799 g         7361 t
ORIGIN      
        1 aataaaacag gataaaggtc ataacccctg aaaacgatcc ggataatatt atccctgcga
       61 gaatcagggc atctgtttta ggcgttttga taccaaactg tttgatgctg agccaaattg
      121 agattaatgc cagagcgaaa aatgccggtt cgaaataaag tgctgtcgtg cgcttgccgc
      181 cgaatttaat gaaattcagc acatagctgt tgctgtaaat cagatatttc gaaatcgact
      241 ccataatact gctgccaccg gtgaggataa tttgcgccat ctccaccgcc gccagcgcca
      301 ccaccagtcc gaccaccaga taaaagaaac gtaatatctt acgatggttg tgtggcgaaa
      361 ttgttttaaa acgaatactc cacaccatgc caataatgat cacaatatag acaaacagca
      421 tagttgaagt gacgtattta ccggcatcca acgactgacc aaacaggtag ttaaacgccg
      481 tgagtcccgc gccaatccct aaggcaatca tcaatttttt aatgctgatg cgttctaaaa
      541 acagcagtaa caagacaggt aaaaaagtga cgatggtgat ggggaaactt tcgccaagct
      601 gggcgatttt gacgttaacc agcaggtaga tcagtggcag cagcaggtag ctacagattc
      661 tgatagaagt tgacatactc ctccagcatc tgttgtccac tgtaggcggc gcgactgcgt
      721 tggctgaact cagccagcgt ggtaccaaat atcgcctgcg cgatttccgg tttgcttaac
      781 tgtaccagtt gcagcacctc ttcttcactg acggttttac caccggattt ttgcagcact
      841 tcccgcgccg catcgctatg ggtggcaatc accggcacgc caatcgatag cgcctcacac
      901 aaaatcagcg ggtagttatc gacacgagaa ctgaacacca gcgcatccat ctgattgagc
      961 gcgctcatca acttgcgctt gtcagtttca aagccgtgat taaccacgtt gccagcggtg
     1021 aacggcgaga acttaccaaa ggtatgcagt tcgattttgt cgcccagcgc catcatctca
     1081 cgcaccagtt gctggttagt tttgccgtcg taacgcaggt catgcgccac cactgcgatt
     1141 ttcggcttgc cctgagtttc gcgcaccgga ggcagatctg ccagaatcgc ttcggttgcc
     1201 atatcaatgc cattattgat aatccggcaa cgccctggcc cgtacagact attgaaagcg
     1261 tcagccacat gctggctggg ggaaataaac tgacagccca gcgccagcat ctcgcggaat
     1321 aactggcgtt tgcccgccac cagctggtgc gcgcgatcaa tcttcaccgg cggatagtta
     1381 tttaaggtcg ggcatttctg gcagcccgtt ttccagcctt cgcaaccgtc ggtaaaggcg
     1441 cagcgtccgg ttacactcca gtggtcgtgc agcgtccaga ccagggtgac atccggttta
     1501 tggtttttca ctttttcgca aaagcgcacc acgctgttaa gattcaacca gtagctgtgc
     1561 agcacatgaa aatgcaggac caccggaccc ggtgtgcgag taatggtgcg atataactca
     1621 ttgaaattgc cgaacagatc gcgattaaac agacgaaaca acgcaatgtt cgccatcgcg
     1681 gtcatccgcg gcgtatgttt gatgacctgc ggatagttct gatggctgac gctctctttg
     1741 ccgcctttgc cgtagccgta gacaaaatgt gacgccagcc cctgttgcag cgcacgctgg
     1801 tggagatcta acgccacacc tgctgccccg ccttccgcca gtcgcacatt aaattgcaga
     1861 atattcattt aatcaccttc actcgcgctt tttctcccac caccagcgcg ttgtccggga
     1921 cagagtcgag caccacgctg cccgcaccca cggtgacgtt gttaccaagc gtgatatcac
     1981 caagaataat gacgttggca ccgagttcga cgccgttgcc aatgtgtgga catgccatgt
     2041 tatcggcacc ccgattgccg atagtgacgc cgtggcgaat ggtgaaatca tcccccgcca
     2101 ccacgttttt attgatcact acggcgtaac cgtgatggat agtaaagcgg cggccaatgg
     2161 tcgcggcggc ctggatttca taaccgaaaa agcattcggt gataatgcga tacagcacca
     2221 gcagcggggc cgcccacaga ttgttgagga cgttcttttt gcgccacacc gaacaaaaat
     2281 gagcgacgcg ataggcaagt accatgcagc acgggcgtaa actccagctg ttggcgcgca
     2341 gatcttccag catctttagc gcccccgaat accatcagcc agacgtttgc cgttacgcac
     2401 cgacagcagc gtcaacaagg tgcgccaggt catacgttta ttgcggatct ggtagagggt
     2461 aaacagctga tattttttac tggcgcggtc gaatttgtct ttgtgcttgc ggtaaaagtg
     2521 gaagtatccg gagaattttt tcggtgacga ggtgatctgc atttcgccgt gattgatatg
     2581 caggatctgc gtcgcctctt ccactttcca cggttcgccg tactccacca ccatccgcag
     2641 gaatatgtcg taatcctgtg ctgctttcag ttcggtatcg aacaaacact ctttgaaacg
     2701 ccatgcccag gtaaagacct gattgccaat gatattacgt ttatagaaca ggcggcgtga
     2761 gtacggcgat ttgggataca gtggtaagct cgccggttgg gaataaactt ctccctagca
     2821 aacgtagtcg ttagcgtaca aaaaggcgtg tgtgaccagt tgctgtttat gggcgaggaa
     2881 gacgctcagg cggttgggcg tccattcatc atcatcatcg atcccggtga tgtattcccc
     2941 ttgcgccagc ataatcgcct ggttacgcac cgcgcacgcc ccgctgttaa tgtcgttgtg
     3001 gatgtaagtg atacgcggat cgttgagggc agtgacgtac tgttgcagtt gttcccagga
     3061 ggtggagcaa tcatccacga tgatcatctc ccagttgctg tagtcctggc gcaggaccga
     3121 ttttatcgcc cgaatcgcca gttgttggcg gttccaggtt ggcatataga ttgagatcag
     3181 cggattgtct ttcataggtt gttctcccgg cttatgagct ggtttgcttg ccggatgcgg
     3241 cgtgagcgcc ttatccggcc tacggggcgg tgcgaatgca ggcgtcgtta tttcgcatcc
     3301 gacttatatt cgtattcgta atagccataa tcctgatacg cgctggcgcg gcggaagatg
     3361 gagttcagaa tcaccccttt caccggaata ccgttttgct caaagcggct cagactggtt
     3421 tccacttctt tcaatgtgtt gaccgcataa cgcgccacca ttaacgtggt tccgacatga
     3481 cgaccaacaa ttgccgcatc ggtcactgcc agaatcggcg gcgtatcaat caacaccagg
     3541 tcatagtttt tactcgccca gttcaccagt tcggcaaagc gttcgctcat taacagttca
     3601 gaaggatttg gcggcacctg accgcgcggg atcaggtcaa atttggcaat agaggtcggt
     3661 ttagcagcgg tagtaatatc gccctgacca atcagaatct ccgacagccc attgacatta
     3721 ttggtgccca acagctcgtg ggtgtagcct ttgcgcatat cgcagtcgat caacaacacg
     3781 cgtttattgg tctggctgat caccgccgcc aggttggcgc agacaaaggt tttaccgatt
     3841 gacgggctaa ccccggtcat catcaacaca ttgttctgcg cctgcatcat cgcgaagtgc
     3901 agactggtac gcagactacg gatggcttca atcgccagat cggttggatt ccccactgcc
     3961 agtagctggc tctgtttata gcgtttaacc cctttgatgg ttttgacgct atcgcgcgct
     4021 ttctgccact ccgacagcgg gatgctggca tagacgctga ttccgtgttc ttccagcact
     4081 tgcgggcttt cgatgccgcg attaaacaac gagcgcagca gcacacccac gatagagagc
     4141 atcaggccaa ggataatcgc gccgaggata atcagccctt tcttcggttt tagcacgcca
     4201 ggctgggtga ttgccgggtc aataatgcgc acatcgccga cggtgctggc ttcggtgatt
     4261 ttcagctcct gctctttatt cagcagttgc atatagacct gctgaccaga ctcgacatcg
     4321 cgggtcagac ggacaatttc ctgctggttt ttcggcatcg ccgttacgcg accgttaagt
     4381 ttggctttct cttcttccag cgcctgacgt ttctccagca gcgtgcggta cgccgggtga
     4441 actttggtgt acagcttgga gatttccgcc tctttaaagg tcagttcgtt caactgcgcg
     4501 tcgatgttca ccatcgaatc gagcaccgct ttcgcttcaa gcggcagatc aacagaatct
     4561 ttatcctgac ggaaggcatt cagtttgttt tcggcaacat cgaggcggct acgtacttcc
     4621 ggtaactgtt gcgcgaggaa agcgaggctt ttcgacgctt ccgccgattt gcgctcaata
     4681 ttttgttcct gatagttacg agcgatgctg ttaagaatgt cgcggatctg ttcgcgatct
     4741 tcaccggtat aagtcaggct cagtacgcct gcgtctttgc cgttctccgt taccgtcagg
     4801 ctgttttgca gttggttgat catccccaac gtggagtatt tggtgacggt aaactcactg
     4861 cccgggcggg cgtgaatggc ttcaaccatc agcgtgacgc cttctttttt cagcatctgg
     4921 cccgcttgcc cacgggcgct aaagccgcca tcactgctca gggtgtagtt tttgtcatcc
     4981 agcacattaa gcgtaaacac ctgatccgcc atctctttcg ggcggttaaa ggtagtcact
     5041 ttcaccgtct cgttctgacg ccccatcagg cgatcccagc ccgccccgaa aatcgggaac
     5101 gtgtttttgc tcaccgcaat atcgaggtcg agatcgtcca ccgttttacc aagcaccaga
     5161 cgcgaacgaa tcaactggat ctcggcgtcc gatgcaggcg gtttgttggc taatgccgag
     5221 ccgatatcct gcaccagcga attgccgctg ttttgctcga tttgtaccag tgcgtcggca
     5281 ctataaatcg gcgtggcgaa gaaagtgtaa accacggcac agagggcaaa tacgacggtg
     5341 atgccaatca cccaccagcg cgcttcaatg acggtgccga ccaggcgacc aatatcgatt
     5401 tcatcactgc ccgttaccgg agcggcatgt tgttttactt tttctgtcat tcttatacct
     5461 gctctgcgtt caatgcctgc gcccactggc gggcagaccg ttcaagtaat gtgtacaccg
     5521 ctgcaaacgt ttcccggctt ttgtgatacg gatcggggat ttcacattcg ttatcccagt
     5581 gaccaaacag catcactttg ccgcgcatct ccggtgccat ctcgcataag cgttcgatat
     5641 ggcgcttatc catggtcaaa atcaggtcat agttgcgaca caggcggcgg ctgatttgtc
     5701 gtgcacagtg gccttccaga gacagttgat gttctgcggc gacgctgata gcggtaggat
     5761 cagcgccctt accgaccagc gcgccgagtc cagcggactc cactttcagt tccgggtgat
     5821 aacgttgcag taagcgttcc gccatcgggg aacggcaaat attgccgaca cagacaacta
     5881 agatgttgtt aaacatgacg attaccagtt atgaatgtcg ctggctgtat ccgtcatgta
     5941 acggacaccg ctaatagttg gcagcaactg attgatcaga cggttccagc gggaaaccgg
     6001 ggcggtggtg acatacacca cgtcataagg ttgcaggcgg aattctgtcg ccatcaccag
     6061 cgacgtcgca tcggacatat ccagctggta gatattggca atcttgccgt tacgcccgcc
     6121 ctcgcctttc agcggacgaa tgacaaagat gccgctggcg ttggaggtgg tcatgtcgat
     6181 accttcggca ttggccagag cttcagtcag ggtcatgccg ctaaagtcca ttttcagggt
     6241 gctctgtttc ttcacttcac ccatcacaaa tactttcaga tcatcattac gcggcacgta
     6301 gagaatatcg ccgggataaa gcagtcggtt ctgattgagg tcgccgtttt gcatcagcgc
     6361 ctgtaaagaa atgcgttctt cacgaccatt gtgtgttagc accacgttgc gccagtcagc
     6421 ggtgtcggtc aggccacctg cggcgttgat ggcgtcgaga atggtcagtg gtacgttggt
     6481 gatcgcctgt tgaccggatt tattcacctg acctgagata taggcctttt gtgagcggaa
     6541 ggcggcgata ttaacgtcca cctgcgggtc agcgatgtac gtcgctaagc gcccggtaat
     6601 atcactgcgg atttcagcga gcgttttccc gactacgtga accttgccga tatacgggta
     6661 aaacatagtg ccgtcaggct gtacccagtt gccggtgtcg ctggagctgc ggtactgacc
     6721 agctggcgtg gtgagttccg ggtgatccca gacggtgaca ttaagaacgt cccccggccc
     6781 gacgcgatac tggtaattcg cgatctcgct ttccagcgtc atattggggc gcgctacatt
     6841 cgggcgtggg cgtaattggt caatcaggcg cggggtcagc ggataaacat tcaccatttt
     6901 gtcgagatcg aaatcagcgt cctgctgttt gatgacgtct ttgcccatcg tcgacatatt
     6961 gctgcccgga agtactgtgc aaccgcttat caaggttact gacaccaata atggcatcaa
     7021 tttcattttg gatttcatca ttgtttattt atcactttgg cagagtaatt atcctgtgca
     7081 ctattaatag caatgtcgcc atgcacattt accttgcagt taattgaata aaaatttaac
     7141 tggcatcagt cctaaaaaaa ttgatttcat ccgcaggcta ttgacagaat aattcagact
     7201 ggtctttcag gcatccagac acgctaccgc ccctggcttt ttagctacca atacactgat
     7261 tgagcttaat ttttcaaacc ctctctgcat acaatcgttg atgagaaagg gttattgcgg
     7321 aaattaactt ccgaatataa ggtgacatta tggtaattga atattggctc gccaataatg
     7381 caggaggaag tgttacagct aacggaatag caggcaaggt aacaattcgg caattggcta
     7441 tttttaagaa ttatattaag tatcattcaa tatggttttt aggagtttct ttaggttgac
     7501 aatatttaat attgcgtctc cacatgcgat atttcttaaa taatgtttta ttattaccgc
     7561 ttttaattca gggataatgt gaggttatta ccctcaaata atatgagata aatagtgctg
     7621 caacattgca tttttgcccc gatttatcca tgatcgaatt gtgacatttg tcatacaacg
     7681 aataggtttt gtacttacta tggaatggat tgccgatccg tctatctggg ccgggttaat
     7741 cacgctgatt gtgatcgaac tggtcctcgg cattgataac ctggtcttta ttgccatcct
     7801 cgccgaaaaa ctaccgccga agcagcgcga ccgcgcacgg gttaccgggc tgctgctggc
     7861 gatgttaatg cgcctgttac tgctagcgtc aatctcctgg ctggtcaccc tgactcaacc
     7921 gctgttcagc ttccgctcgt ttacctttag cgcccgcgac ttaatcatgc tgtttggtgg
     7981 tttcttcctg ttgttcaaag ccacgatgga gctgaacgaa cggctggaag ggaaagacag
     8041 caataatccc acacaacgca aaggtgcgaa gttctggggc gtggtgacgc aaattgtggt
     8101 actggacgcc attttctcac tcgactcagt gattaccgcc gtcgggatgg tcgaccattt
     8161 actggtcatg atggccgccg tggttatcgc tatcagcctg atgttgatgg ccagcaagcc
     8221 gttaacgcaa tttgttaaca gtcacccgac catcgttatt ctctgcttaa gcttcctgtt
     8281 gatgattggc tttagcctgg tggcagaagg tttcggcttc gtcattccga aaggctacct
     8341 gtacgctgcc attggtttct cggtgatgat cgaggcactc aatcagttgg ctatctttaa
     8401 ccggcgacgt tttctttccg ctaatcagac gctacgccag cggactaccg aagcggtaat
     8461 gcgcctgctt agcgggcaaa aagaagatgc ggaactggac gccaaaaccg cgtccatgct
     8521 ggtggatcat ggaaaccagc agatctttaa tccgcaggaa cggcggatga ttgagcgggt
     8581 acttaatctt aaccagcgta ccgtaagcag cattatgacg tcgcgccacg atattgagca
     8641 tatcgatctc aacgcgccgg aagaagaaat ccgccagttg ctggagcgaa atcagcatac
     8701 gcggctggtg gttaccgacg gtgatgacgc agaagatttg ctcggtgttg ttcacgttat
     8761 cgacctgcta caacagtcac tgcgcggcga accgctcaac ctgcgggtgt tgattcgcca
     8821 gccgctggtg ttcccggaaa ccttgccgtt gttaccagcc ctggagcagt tccgtaatgc
     8881 ccgcacgcac tttgcttttg tggtggatga gtttggctcg gtggaaggga ttgtgacatt
     8941 aagtgacgtc actgaaacca ttgccggtaa cttaccgaac gaagtggaag agatcgacgc
     9001 ccgccatgat attcagaaga atgcggacgg ttcctggacg gcgaatggtc atatgccgct
     9061 ggaggatctg gtgcaatatg tgccgctgcc gctggatgaa aaacgtgaat atcacaccat
     9121 tgccgggctg ttgatggagt atttgcagcg tattccaaaa cctggcgaag aagttcaggt
     9181 gggggattat ttgcttaaaa cgttgcaggt ggaaagccat cgcgtgcaga aggtgcagat
     9241 tataccgctg cgtaaggatg gcgagatgga gtacgaggtg tgatggtatc ggccatttta
     9301 cccaatgctg cgtaacgcct tataacttca gcaatgaagc gttgtctgtg gtaaacggac
     9361 atcacagcgg gggtgtgggt ccggctgtaa atcgccgaat cgtttcctgt aggctggggc
     9421 agcccacatg gatgtgggct gagggcgcgt tttacaggga tgttacctcg cgcccgtacc
     9481 cgatagccga aaggaataag atgagggaac cgcgccagcg gcgatttacc gccgggagcc
     9541 cgggttgcca gggtggtggc gattgagcca ccctggcacg ttcaccggct tgagcgttcc
     9601 atagtagcga ggaacatcaa gtgaacggaa cagccttcga tcttacatat tcagaatata
     9661 atagtgccgc cgcgaacgcc tgatgcgacg ctttacgcgt cttatcaggc ctacaggaca
     9721 ggtcacctgg taacctacat cttctccagc aacttcttca catctttgcc attgcgggaa
     9781 tctttattcc gctcggccca gtcattcagg cgacgtttcg cttcgtcctg tagatgtttg
     9841 cgcagtaact gatccacttg caggctgtaa ttgagttgct gccagttgcc ataaacccgc
     9901 agcggtactg gcgtttcttt gaggaaatca atcagtttgc tttccccgtt ccagccaccc
     9961 acgacccgaa tatcaaactg ggtgtcgcag gtttgatctg ccagattcaa catgccttcc
    10021 cctgtcagcg ccagcacagg cgattgacct tgcatgtcgt ttaacgtcac gacgccatcc
    10081 ttcaacgtca aatcggtggt aaagcggtca agccgcgtca cgttatcgaa gttttcagcg
    10141 gccttcacat caccgccatt acgctctacc gcttgctgaa tcatctgctg gaagttcatc
    10201 ccttccatac gtgtgtcggt catctcaaca tgcgcctgtc cttgccagtt gtggcggaat
    10261 gcgtcggcat ctatgtcagc accggagaaa tcaccagcca gtgacatttt tccggtcaac
    10321 gaaatcggat agttaaacgc cttcagaatg gtaccaatct caacgttctc cagccgtggc
    10381 tggaagttta tccgcggatt tattgatgtc gcgtccagcg tgcccggcag tgaaaccttt
    10441 ccaccgttaa gcttgccctg cagttgagta atttccagca aacccgactt gttggtcatt
    10501 tgcgtggcaa catctgtaaa attcattccg cgccagcgca cgttactggc ctgcaacaaa
    10561 atatcagctg taaagccttg cagtccctga taggccggtt catcaatacg cgaagaaatg
    10621 accgggcgcg gcaacgttga ttggctctgc ccctgctgcg cggcaccgtt ttcaccattc
    10681 gcggtttcat taagcgggat gaggttgtca agattcagtt gcgggaattg cagtctcagc
    10741 tgccattccg gtttctctgt cagcgtgacc tgtgcttgtc cgctcagcgt actgtcattg
    10801 gcggtcaaac taatttggtt aaatgaaagg cgtttatgtg actcctgcca ctgggactgg
    10861 aaactcccct gcccctgaat accttgtttt ggtaaatcgg caccctgcaa ctgccagtta
    10921 atttgttcaa tagccgctgt taaatcatgc ggataatcag aagcatctac cgtaccgtta
    10981 agggatatcg tgagatcgcg ctgatcgcga ttaacccgcc cggagaactc aaatgagcca
    11041 cgatgttggg gatcttgttc catttgcagg cggatattgc ggattgtcac ctgctcgtca
    11101 tcttcatgct ggaacaccag aacgctgtcc gccaccttaa gactggatat atcaaacgac
    11161 catccgcgat cgtctgacag atccggcaag gtattgtcgc gcggtgcaac cggagcgtct
    11221 tcactgcgca ccgcttccgt ctgcggcgtc agttggatca ctgccccttt tagcatcacc
    11281 tgcttaacgc tcagttgatg actcagtagt ggtaaaagcg ccacgtccag acgcatgttg
    11341 tcggcgcgaa ccagtggctg gcttgcgccc tgggcggtga gagacattcg cccggagagg
    11401 atactaagct gcggccagac gtgccaacgc agtggcccgt cgagctgcaa ttgataaccg
    11461 ctacgtgcag caacttgctt gaccatatag tcgcggaaat cattcggatt caccagcaac
    11521 actaacgcag ataacccggc caccagcacg accaagagta tcatcagcgt cgtcagaaat
    11581 cgtctcatgg tatcctcaat gggcctgaat tagtctttat cgattcggct ggctaccgcg
    11641 ccctgctggt tgcgatattt cgcatcttca cggcggttgt aaggtcgcgc cgccgggccg
    11701 gaaagcggct caaagctcag cgcaccaatt aacatgcccg gacgcagcgc cagcggcagc
    11761 ttaccggagt tgtagaactc cagcacaatg caaccagacc agcccggatc gatacggtgc
    11821 gcggtgacgt gcaccatcag ccccagacgc gccaaagagg aacgcccgtc cagccagccc
    11881 accagatcgg ctggcagcgt caccgactcc agtgtcaccg ccagcgccag ctctcctggg
    11941 tgaagataga acgcctcgcc ctcgtcgaga acgatctcat cgctcatcac gcggtcaagc
    12001 gcggcgctca cttcatcttt gggaccgctc agatcgataa acgctgccgt gtgaccacgg
    12061 aaggtacgaa atttattgcc caggcgtaca tccaccgtcg cgccgttaat acgctccact
    12121 ggcggacgtg ggttgatcga caaacggcct tcatcaagcc aggcttcaat atctcggtca
    12181 cacagacgca tggcattttc tcctttcgca catcactccc ttaacgccaa ttacgtcaag
    12241 ggcataccaa cttatcactg aacggtacac aatttatcaa gcttattcaa agaactgact
    12301 tattttcgct ttcaatatat cgatcgcgat gcggtttttc ccgccgcgcg gcacgataat
    12361 gtccgcatat tgtttagaag gctcaatgaa ttgcaggaac atcgggcgca cggttttttg
    12421 atattgcgcc atcacggaat ccattgaacg cccacgctcg ttaacgtcac gcttgatgcg
    12481 gcgcatcagg cagatatcca gcggggtatc aacgaaaatg gagaagttaa gttcgtcacg
    12541 caaacgcgca tccgtcagca acaaaatgcc ttcgagaatg atgaccttct tcggctcaac
    12601 cgtcaccgtt tctttcatac gcgtatgttc aacatagctg taaaccggca ggtcaattgc
    12661 cgagccgcgt ttcaacgctt gtaaatgctc aagcaacagg ctgtgatcca tcgcgctggg
    12721 atggtcgtag ttggtcttaa cgcgttcttc catcgacaga tggctttgat ctttgtaata
    12781 gcagtcttcg ggaattacgc cgatgtgttc atcaccgact tgctcacgta attcacgata
    12841 aagggtactg gcaataagac tcttgccgga agccgatgcg ccagcgatac cgataatgac
    12901 gcactgatga gactgatcag tcatatattt agcgacctga ttaacctgga tgttaggaag
    12961 ggggcgacga agcgccaaac gcggcaatta tagggatttc atccgtctga taccagtcga
    13021 atagcgttgc cgcgcgctca gagttaattg ttgacaaaga attcccggtg gcaaattacg
    13081 ttgatcagtt ttatgtaagg taaaaaatgt tatacgcagt tgcgcaaatt atccgccttt
    13141 acgtcacttt atgagcaatt cgcatataaa atgtaaaact tttgtactag cataaacaca
    13201 gaaacgaata ctggcgacct ggccttgcgg ataaagcggt aataagcaaa caatcacagc
    13261 atgtattaat tgccctgccc cacccgctgc ttcacctggt cagtttaggt ttagtctcgt
    13321 ttatctttac ccttgtctcg cttgagcttt cgcagtttgg cacccaactc gccccactgt
    13381 ggttcccgac gtccatcatg atggtggcgt tttatcgcca tgccgggcgc atgtggccgg
    13441 gaattgcgct gagctgttcg ctgggaaata tcgccgcatc cattccagga aggccagcag
    13501 gtcaaagcag gcgatttact ggcagaaatt gaccccagcc agttcaaagt tgcattagca
    13561 caaacccagg gccaactggc aaaagataaa gccacgcttg ctaacgcccg ccgtgatctg
    13621 gcgcgttatc agcaactggc aaaaaccaat ctcgtctccc gtcaggagct ggatgcccaa
    13681 caggcgctgg tcagtgaaac cgaaggcacc attaaggctg atgaagcaag cgtcgccagc
    13741 gcgcagttgc aactcgactg gagccgcatc accgcaccag tcgatggtcg cgttggtctc
    13801 aagcaagttg atgttggtaa ccaaatctcc agtggtgata ccaccggaat tgtggtgatc
    13861 acccagacgc atcctatcga tttagtcttt accctgccgg aaagcgatat cgctactgta
    13921 gtgcaggcgc aaaaagccgg aaaaccgctg atggtagaag cctgggatcg caccaattcg
    13981 aagaaattaa gtgaaggcac gctgttaagc ctcgataacc aaatcgatgc cacaaccggt
    14041 acgattaaag tgaaagcacg ctttaataat caggatgatg cgctgtttcc caatcagttt
    14101 gttaacgcgc gcatgttagt cgacaccgaa caaaacgccg tagtgatccc aacagccgcc
    14161 ctgcaaatgg gcaatgaagg ccattttgtc tgggtgctga atagcgaaaa caaggtcagc
    14221 aaacatctgg tgacgccggg cattcaggac agtcagaaag tggtgatccg tgcaggtatt
    14281 tctgcgggcg atcgcgtggt gacagacggc attgatcgcc tgaccgaagg ggcgaaagtg
    14341 gaagtggtgg aagcccagag cgccaccact ccggaagaga aagccaccag ccgcgaatac
    14401 gcgaaaaaag gagcacgctc ctgatgcagg tgttaccccc gagcagcaca ggcggcccgt
    14461 cgcgcctgtt tattatgcgt cctgtcgcca ccacgctgct gatggtggcg atcttactcg
    14521 ccgggattat cggttatcgc gccctgcccg tttcggcgct gccggaagtg gactatccga
    14581 ccattcaggt ggtcacgctc tacccaggtg ccagcccgga tgtcatgacc tctgccgtta
    14641 ccgcgccgct cgaacgccag ttcgggcaga tgtctggcct gaaacagatg tcgtcgcaaa
    14701 gttccggcgg tgcgtcagtt atcactttgc agttccagct aacattaccg ctcaatgtcg
    14761 ccgagcagga agtgcaggcc gcgattaacg ctgcgaccaa cttgttgccg agcgatctgc
    14821 ctaacccgcc ggtttacagc aaagtgaacc cggcagatcc gccgatcatg acgctcgccg
    14881 tcacctcaac cgccatgccg atgacgcaag tggaagatat ggtggaaacc cgcgtcgcgc
    14941 agaaaatctc gcagatttcc ggcgtcggcc tggtgacgct ttccggcggt cagcgtccgg
    15001 ctgttcgcgt caaacttaac gctcaggcga ttgccgccct cggcctgacc agcgaaaccg
    15061 tgcgcaccgc cattaccggc gctaacgtta actcggcaaa aggtagcctc gacggccctt
    15121 cccgtgcggt cacgctttcc gcgaacgacc agatgcaatc cgccgaagag tatcgccagc
    15181 taatcatcgc ctaccagaac ggcgcgccaa ttcgtctggg cgatgtcgca actgtagagc
    15241 aaggtgcaga aaacagctgg ctcggcgcgt gggcgaacaa agaacaggcc attgtgatga
    15301 atgttcagcg ccagcccggt gctaacatta tctccaccgc cgacagcatt cggcagatgc
    15361 tgccacagct cactgagagt ctgccgaaat cggtgaaggt gacagtgctt tccgatcgca
    15421 ccaccaatat ccgcgcatcc gtcgatgata ctcagtttga attgatgatg gctatcgcgc
    15481 tggtagtcat gattatctac ctgtttttgc gcaatattcc ggcgaccatc attcccggtg
    15541 ttgctgtacc gctgtcgtta atcggcactt tcgcggttat ggtgtttctc gatttttcaa
    15601 tcaataacct gacactgatg gctttaacta tcgccaccgg attcgtggtc gatgacgcca
    15661 tcgtggtgat cgaaaacatt tcccgctata tcgaaaaagg cgaaaaaccg ttggcggcgg
    15721 cgctcaaggg cgcaggtgaa atcggcttta ccattatctc gctgaccttc tcactgattg
    15781 cggtgttgat cccactgctg tttatgggcg atatcgtcgg gcgactgttc cgcgaatttg
    15841 ctattaccct ggcggtagcg attttgatct cagcggtggt gtcgctgacc ctgacaccga
    15901 tgatgtgcgc gcggatgctc agccaggagt cgttgcgtaa acagaacagc ttctcccgtg
    15961 cctcggaaaa aatgttcgac aggataatcg ccgcctatgg tcgtggactg gcgaaagtgc
    16021 tgaatcatcc gtggctgacc ttaagcgtgg cactcagcac gctgctgctt agcgtgctgc
    16081 tgtgggtgtt cattccgaaa ggtttcttcc cggtacagga caacggcatt attcagggca
    16141 ctttgcaggc accgcaatcc agctcctttg ccaatatggc ccagcgacaa cgccaggtcg
    16201 cggacgtgat tttgcaggat ccggcagtgc aaagcctgac ctcatttgtt ggcgttgatg
    16261 gcactaaccc gtcgctgaat agtgcacgtt tacaaatcaa cctcaaaccg ttggatgaac
    16321 gtgatgatcg ggtgcaaaaa gtcatcgccc gtctgcaaac ggcggtagat aaagtgccgg
    16381 gcgtcgatct cttcctgcaa ccaacgcagg acctgaccat cgatactcag gtcagccgca
    16441 cccagtacca gtttaccttg caggccacgt cactggatgc gctcagtacc tgggtgccac
    16501 agttgatgga aaaactccag caactgccac agctttctga tgtctccagc gactggcagg
    16561 acaaagggct ggtggcgtat gtcaatgttg atcgcgacag cgccagccgt ctggggatca
    16621 gcatggcgga cgtcgataac gccctgtaca acgcgtttgg tcagcgactg atttccacta
    16681 tttatactca ggctaaccag tatcgcgtgg tgctggagca caataccgaa aataccccag
    16741 gcctcgcggc gctggatacc attcgcctga ccagcagcga cggtggcgtg gtgccgttaa
    16801 gctcaattgc caaaattgag cagcgttttg cgccgctttc catcaaccat ctggatcagt
    16861 tcccggtaac aaccatctcc tttaacgtgc cggataacta ttcgctgggc gatgcggtgc
    16921 aggcgattat ggacaccgaa aagacgctga atctgccggt ggatatcacc acgcaattct
    16981 agggcagcac cctcgccttc cagtcggcgc tgggcagcac tgtctggctg attgtcgcgg
    17041 cggtggtggc gatgtatatc gtgctcggca ttctgtacga gagctttatt cacccgatca
    17101 ccattctctc gacgctaccc accgcagggg tcggcgcgct gctggcatta atgattgccg
    17161 gtagcgaact ggatgtgatt gcgattatcg gcattatttt gctgatcggt attgtgaaga
    17221 agaacgccat catgatgatc gacttcgcgc tggctgctga gcgcgagcaa ggcatgtcgc
    17281 cgcgcgatgc aatctaccag gcttgtctgt tgcgttttcg tccgatcctg atgaccaccc
    17341 tggcggctct gcttggcgcg ctgccgctga tgttgagtac cggggtcggc gcggaactgc
    17401 gtcgtccgtt aggtatcggc atggtcggcg gtctgattgt cagccaggtg ctgacgctgt
    17461 tcaccacgcc ggtgatttat ctgctgttcg accgcctggc attgtggacc aaaagtcgct
    17521 ttgctcgcca tgaagaggag gcgtaagtga agttttttgc cctcttcatt taccgcccgg
    17581 tggcgacgat tttactgtcg gtcgccatta ccctgtgcgg catactgggt ttccgtatgc
    17641 tgccggtcgc cccgctgccg caggtcgatt ttccggtgat tatggtcagc gcctcgctgc
    17701 ccggtgcgtc gccggagaca atggcgtctt ccgttgccac gccgctggag cgctcacttg
    17761 ggcgcattgc cggggttagt gaaatgacct ccagcagttc gctcggcagc acgcgcatta
    17821 ttttgcagtt tgattttgac cgggatatca acggcgcagc gcgtgatgtg caggcggcga
    17881 tcaacgctgc acaaagtttg ctgcccagcg ggatgcccag ccgcccgacc tatcgcaaag
    17941 cgaacccgtc ggatgcgcca attatgatcc tcacgctgac atccgatact tattcgcagg
    18001 gtgaactgta cgatttcgcc tcgacgcagc tggctccgac gatttcgcaa atcgacggtg
    18061 tcggtgatgt cgatgtcggt ggcagctcgc tgcccgccgt gcgcgtcggg ctgaccccgc
    18121 aggcgctgtt taatcagggc gtgtcgctgg acgacgtacg cactgccatc agcaatgcca
    18181 acgtgcgtaa accgcagggg gcgctggaag atggcactca ccgctggcag atccagacca
    18241 acgatgaact gaaaaccgcc gctgaatatc agccgctgat tattcactac aacaacggcg
    18301 gcgcggttcg tctgggcgat gtggcgacgg tgactgactc agtgcaggat gtgcgcaacg
    18361 ccgggatgac caacgccaaa ccggctattt tactgatgat ccgcaaactg ccggaagcca
    18421 atattatcca gacggtagac agcatccggg caaaattacc agagttgcag gagaccattc
    18481 cggcggcgat tgatctgcaa attgcccagg atcgctcccc caccattcgc gcctcgctgg
    18541 aagaagtcga gcaaacgctg attatctcgg tggcgctggt gattctggtg gtctttttat
    18601 tcctgcgctc gggtcgcgcc actattattc ccgccgttgc ggtgccggtt tcgctgattg
    18661 gtacgtttgc ggcgatgtac ctgtgcggat tcagtctcaa taatctttcg ctgatggcgc
    18721 tcaccattgc tactggtttc gtggtggatg acgccattgt ggtgctggaa aacattgccc
    18781 gtcatctgga agcggggatg aaaccactac aggccgcgct gcaaggtact cgcgaagtcg
    18841 gctttacggt gctgtcgatg agtctgtcac tggtggcggt gttcctgccg ctgctgttga
    18901 tgggcggatt gccgggccga ctgttacgcg agtttgccgt gacgctttct gtcgccattg
    18961 gtatttcgtt gctggtttcc ctgacgctaa cgccaatgat gtgcggctgg atgctaaaag
    19021 ccagcaagcc gcgtgagcaa aagcgactgc gtggttttgg ccgcatgttg gtagccctgc
    19081 aacaaggcta cggcaagtcg ctgaaatggg tgctcaatca tacccgtctg gtgggcgtgg
    19141 tgctgcttgg caccattgcg ctgaatatct cgatcccgaa aaccttcttc ccggagcagg
    19201 acaccggcgt actgatgggt gggatacagg cggatcagag tatttcgttt caggcgatgc
    19261 gcggcaaatt gcaggatttc atgaaaatta tccgtgacga tccggcagtg gataatgtca
    19321 ccggctttac cggcggttca cgagttaaca gtgggatgat gtttatcacc ctcaagccac
    19381 gcgacgaacg cagcgaaacg gcgcagcaaa ttatcgaccg tctgcgcgtg aagctggcga
    19441 aagaaccggg ggcgaatctg ttcctgatgg cggtacaaga tattcgcgtt ggtgggcgtc
    19501 agtcgaacgc cagctaccag tacacgttgt tatccgacga cctggcggca ctgcgcgaat
    19561 gggagccgaa aatccgcaaa aaactggcga cgttgccgga actggcggac gtgaactccg
    19621 atcagcagga taacggcgcg gagatgaatc tggtttacga ccgcgacacc atggcacggc
    19681 tgggaatcga cgtacaggcc gccaacagtc tgttaaataa cgccttcggt cagcggcaaa
    19741 tctcgaccat ttaccagccg atgaaccagt ataaagtggt gatggaagtg gatccgcgct
    19801 atacccagga catcagtgcg ctggaaaaaa tgttcgttat caataacgaa ggcaaagcga
    19861 tcccgctgtc gtatttcgct aaatggcaac cggcgaatgc cccactatcg gtgaatcatc
    19921 agggattatc ggcggcctcg accatctcgt tcaacctgcc gaccggaaaa tcgctctcgg
    19981 acgccagtgc ggcgatcgat cgcgcaatga cccagcttgg tgtgccttcg acggtgcgcg
    20041 gcagttttgc cggcacggcg caggttttcc aggagacgat gaactcgcag gtgatcctga
    20101 ttatcgccgc catcgccacg gtgtatatcg tgctgggtat cctttacgag agttacgtac
    20161 atccgctgac gattctctcc accctgccct cggcgggcgt tggagcgctg ttggcgctgg
    20221 agctgttcaa tgccccgttc agcctaatcg ccctgatagg gatcatgcta ttaatcggca
    20281 tcgtgaagaa aaacgccatt atgatggtcg attttgcgct tgaagcccaa cggcacggta
    20341 acctgacgcc gcaggaagct attttccagg cctgtctgct gcgttttcgc ccgattatga
    20401 tgaccaccct ggcggcgctg tttggcgcgc tgccgctggt attgtcgggc ggtgacggct
    20461 cggagctgcg gcaacccctg gagatcacca ttgtcggcgg tctggtgatg agccagctcc
    20521 tgacgctgta taccacgccg gtggtgtatc tctttttcga ccgtctgcgg ctgcgttttt
    20581 cgcgtaaacc taaacaaacg gtaaccgagt aaatgacaga tcttcccgac agcacccgct
    20641 ggcaattatg gattgtggct ttcggcttct ttatgcagtc gctggacacc accatcgtaa
    20701 acaccgccct tccctcaatg gcgcaaagcc tcggggaaag tccgttgcat atgcacatgg
    20761 tcattgtctc ttatgtgctg accgtggcgg tgatgctgcc cgccagcggc tggctggcgg
    20821 acaaagtcgg cgtgcgcaat attttcttta ccgccatcgt gctgtttact ctcggttcac
    20881 tgttttgcgc gctttccggc acgctgaacg aactgttgct ggcacgcgcg ttacagggcg
    20941 ttggcggtgc gatgatggtg ccggtcggca ggttgacggt gatgaaaatc gtaccgcgcg
    21001 agcaatatat ggcggcgatg acctttgtca cgttacccgg tcaggtcggt ccgctgctcg
    21061 gtccggcgct cggcggtctg ctggtagaat acgcgtcgtg gcactggatc tttttgatca
    21121 acattccggt ggggattatc ggtgcgatcg ccacattgat gttaatgccg aactacacca
    21181 tgcagacgcg gcgctttgat ctttccggct ttttattgct ggcggttggc atggcggtat
    21241 taaccctggc gctggacggc agtaaaggta caggtttatc gccgctggcg attgctggcc
    21301 tggtcgcagt tggcgtggtg gcactggtgc tttatctgct gcacgccaga aataacaacc
    21361 gcgccctgtt cagtctgaaa ctgttccgca ctcgtacctt ttcgctgggc ctggcgggga
    21421 gctttgccgg acgtattggc agtggcatgt tgccctttat gacaccggtt ttcctgcaaa
    21481 ttggcctcgg tttctcgccg tttcatgccg gactgatgat gatcccgatg gtgcttggca
    21541 gcatgggaat gaagcgaatt gtggtacagg tggtgaatcg ctttggttat cgtcgggtac
    21601 tggtagcgac cacgctgggt ctgtcgctgg tcaccctgtt gtttatgact accgctctgt
    21661 tgggctggta ctacgttttg ccgttcgtcc tgtttttaca agggatggtc aactcgaccc
    21721 gtttctcctc catgaacacc ctgacgctga aagatctccc ggacaatctg gcgagcagcg
    21781 gaaacagcct gctgtcgatg attatgcaat tgtcgatgag tatcggcgtc accatcgccg
    21841 ggctgttgct ggggcttttt ggttcgcagc atatcagcgt cgacagcggc accacacaaa
    21901 ccgtctttat gtacacctgg cttagcatgg cgtttatcat cgcccttccg gcgttcatct
    21961 ttgccagagt gccgaacgat acgcatcaaa atgtagctat ttcgcggcga aaaaggagcg
    22021 cgcaatgaag ttctggcgac ccggtattac cggcaaactg tttctggcga ttttcgccac
    22081 ctgcattgtc ttgctgatca gtatgcactg ggcggtgcgt atcagttttg agcgtggctt
    22141 tattgattac atcaaacacg gcaatgaaca gcgattacag ctgttaagtg atgcgcttgg
    22201 cgagcagtat gcgcagcatg gtaactggcg cttcctgcgc aacaacgatc gctttgtctt
    22261 tcagatcctg cgttcatttg aacacgataa ttcggaagat aaacccggcc cgggtatgcc
    22321 gccgcacggc tggcgtaccc agttctgggt ggtcgatcaa aacaacaaag tactggttgg
    22381 tccgcgagcg ccgattccac ctgacggtac acggcgaccc attctggtca acggtgcgga
    22441 agtgggcgcg gtgattgcct cccctgttga gcggctgacc cgcaatactg atatcaattt
    22501 cgataaacaa cagcggcaaa ccagctggct gatcgtcgcc ctggcaacgt tactcgcggc
    22561 gctcgccacc tttctgctgg cgcgcggttt gctggcaccg gtaaaacgac ttgtcgatgg
    22621 cacgcacaaa ctggcggcgg gcgatttcac tacccgcgta acgcccacca gtgaagatga
    22681 actgggcaaa ctggcgcaag acttcaacca gctcgccagc acgctggaga aaaaccagca
    22741 aatgcggcgc gattttatgg ccgatatttc tcacgaactg cgtacgccat tagcggtgct
    22801 gcgcggtgaa ctggaagcca ttcaggatgg cgtgcgcaaa ttcacgccgg agacggtggc
    22861 gtctttacag gcggaggtcg gtacactgac caaactggtt gacgatcttc atcagttgtc
    22921 gatgtctgat gaaggcgctc tcgcctatca aaaagcaccg gtagatttga tcccactgct
    22981 ggaagtggcg ggcggcgcat ttcgcgaacg attcgccagc cgtggcctga aactgcaatt
    23041 ttccctgcca gacagtatca ccgtatttgg cgatcgcgac cgtttaatgc agttattcaa
    23101 taacttactg gaaaacagcc tgcgctacac tgacagcggc ggcagcctga aaatctctgc
    23161 cgagcagcac gacaaaacgg tgcgcctgac ctttgccgac agtgcgccag gtgtcagtga
    23221 cgatcagcta caaaaattgt ttgaacgttt ttatcgcacc gaaggttccc gcaaccgcgc
    23281 cagcggcggt tccgggctgg ggctggcgat ttgcctgaac attgttgaag cccataatgg
    23341 ccgcattatt gccgcccatt cgccttttgg cggggtaagc attacagtag agttaccgct
    23401 ggaacgggat ttacagagag aagtatgacc gagttaccaa tcgacgaaaa cacaccgcgt
    23461 attttgatcg tggaagatga accgaagctg gggcagttgc tcattgatta tctgcgtgct
    23521 gcgagctatg cgccgacgct tatcagccac ggcgatcagg tactggcgta tgtgcgccag
    23581 acaccaccgg atctgatcct gttggatctg atgctccctg gcaccgatgg cctgacatta
    23641 tgccgggaaa ttcgtcgttt ttccgatgtt ccgatcgtga tggtgacggc aaaaatcgaa
    23701 gagatcgacc gcctgctggg gctggagatt ggcgccgatg actacatctg caagccgtac
    23761 agcccacggg aagtggtagc gcgtgtcaaa accattttgc gccgttgtaa accgcaacgc
    23821 gaacttcagc aacaggatgc cgaaagcccg ttgattatcg acgaaggtcg ttttcaggct
    23881 tcatggcgcg gtaaaatgct tgacctgacg cctgcggaat ttcgtctgct taaaacgctc
    23941 tctcacgaac caggaaaagt gttctcccgc gagcaattgc tcaatcatct ttatgacgac
    24001 taccgcgtag taaccgaccg caccattgac agccacatta aaaatctgcg ccgcaagctg
    24061 gaatctctcg acgccgaaca gtcatttatc cgcgccgttt acgccgttgg ttaccgctgg
    24121 gaagccgacg cctgccgcat cgtttagttt tagcgacatt actttgttag ccggagatgg
    24181 cgttcagacc cgatccggca acattattcc acgcatggtc agcaaactga cattatctct
    24241 ccgccctggc aggcatcaga aaatcgctca tactttaatc agtaaacagc acctttagat
    24301 gctgttttcg ataagcaatt tcaatcaagg agtcattatg gctggttggt ttgaactcag
    24361 taagagcagt gataatcagt tccggtttgt gctaaaagcg ggcaatggtg agactatcct
    24421 caccagcgag ctgtatacct caaaagcctc tgcggaaaag ggcatcgcgt cggtgcgtag
    24481 caacagcccg caagaagagc gctatgagaa aaaaacggca agcaacggca aattctattt
    24541 caatctgaaa gccgctaatc atcaaattat cggctccagc cagatgtacg ccaccgcgca
    24601 atctcgtgaa accggaattg cctccgttaa agccaatggc acaagccaga cggtgaaaga
    24661 caatacgtaa ttacgatgcc gggcgtgggt tgttggcgtc cggcgcttta ccagatcccc
    24721 cctacagcgc tacaatgccc gcccttaaag tgggggcctc ccctaaccgc ttcatcaggt
    24781 gaagcggatc tgacctgtca tcagaacgag agaattatgt ttaaaccgga actcctttcc
    24841 ccggcgggaa cgctgaaaaa tatgcgttac gctttcgctt atggcgcaga tgctgtttat
    24901 gcgggccagc cgcgttactc cctacgtgtg cgcaacaacg aattcaacca cgaaaatctt
    24961 cagctcggca tcaatgaagc ccacgcgctg gggaaaaagt tttatgtcgt ggtcaacatt
    25021 gcaccgcaca acgccaagct gaaaaccttt atccgtgacc tgaaaccggt ggtggaaatg
    25081 gggccggatg cgctgattat gtccgatcca gggctgatta tgctggtgcg tgagcacttc
    25141 cctgaaatgc cgatccacct ctcggtgcag gctaacgccg tgaactgggc gacggtgaaa
    25201 ttctggcagc aaatgggact gacccgcgtg atcctctctc gcgagctgtc gctggaagag
    25261 attgaagaga tccataatca ggtgccggat atggagatcg agatcttcgt tcacggcgca
    25321 ctgtgcatgg cctactccgg tcgctgcctg ctctctggct atatcaacaa gcgcgacccg
    25381 aaccagggca cctgcaccaa cgcctgccgc tgggagtaca acgtccagga agggaaagaa
    25441 gatgacgttg gcaacatcgt acacaagtac gagccgattc cggtgcaaaa tgttgagccg
    25501 acgctgggta tcggcgcgcc aaccgacaaa gtgtttatga tcgaagaagc ccagcgtccg
    25561 ggcgagtata tgaccgcgtt tgaagatgag cacggcactt acatcatgaa ctcgaaagat
    25621 ctgcgcgcca tcgcccatgt agaacgcctg accaaaatgg gcgtgcattc gctgaaaatc
    25681 gaaggtcgta ccaaatcttt ctactattgc gcacgcaccg cacaggttta ccgcaaagct
    25741 atcgatgacg ccgctgcggg aaaaccgttc gataccagcc tgctggaaac tctggaaggt
    25801 ctggcgcatc gtggctatac cgaaggtttc ctgcgtcgtc atactcacga cgattatcag
    25861 aactacgaat acggttattc agtttctgac cgccagcagt ttgttggtga gtttaccggt
    25921 gagcgcaagg gggacctcgc agcggtagcg gtgaaaaata aattctccgt tggcgacagt
    25981 cttgagctga tgacgccgca aggcaacatt aactttaccc ttgagcacat ggaaaacgcc
    26041 aaaggcgaag ctatgccggt cgcaccaggc gatggttata ctgtgtggct cccggtcccg
    26101 caggatcttg agctaaatta cgcgctgcta atgcgtaatt tctccgggga aaccacgcgt
    26161 aacccccacg gtaagtgatt aatttcgatt atttttcccg gatggaaaat tcttagaaac
    26221 cgatcacata cagctgcatt tattaaggtt atcatccgtt tcgctgaaaa acataatcca
    26281 taaaatgcta gctgtaccag gaaccacctc cttagcctgt gtaatctccc ttacacgggc
    26341 ttattttttg tacataagct gtaaagaagg attgatccct gataaccgtc ttccattgac
    26401 cacatcgata gaatccccct tcatagcaca atgcctttca cgtaacggta tcgtgctcgc
    26461 ccaggttccg gctacgcaca gccagaacgc gcatgtttga cgcttacaaa aaaatattct
    26521 cactctccac atttgaatgt cagacgagcg acgccatgta atcctgcacc ttctgtcttc
    26581 aggtcaacta tctgcatttt tttgccctga gtaacacaga aatgggctgc atcatttttt
    26641 actatatttt ctgcaccaga tattctgccc ctggctaaag aagcttcggc ttcggtgtag
    26701 tattggttat cgagtttacg ctgaatatta cttttatatg caagaccaaa tttaccgata
    26761 cttgtttcat cattatgcac agcacaacca gatataagaa aaatactaat taatgatata
    26821 gcagctatct ttttcatctc accttccccc attaaatacc aacgacactc tctagtgttt
    26881 aaatataata atggcatgat tattataatt gaataggatt ataataaatg ttctgtacaa
    26941 catttcctac ataagtagga attacggaca ttgaggccct tcagggtaac tccatggggg
    27001 ctttaatata ttatattgaa gatgccactg tttagttgaa tattaggtat atgctctttt
    27061 ttgaaattta tcggtggcag ccgttagtat tcgctgtccc cattgcaagc tcctggtggt
    27121 aaccactgaa tcctccatac ttgaactgac tttttatcct ccgactttca tcctgttctg
    27181 actccacctt ttgttttctg ctctacacta tctacagacc aatcataaag gcacatacga
    27241 tcatggcaga atttcccgcc agcttactga ttcttaatgg taaaagtact gacaatctac
    27301 ccttgcgcga agcaattatg ctgttgcgtg aggaaggaat gacgatccat gtgcgggtca
    27361 cctgggagaa aggtgatgcc gcacgatttg tagaggaggc ccggaagttg ggcgtcgcaa
    27421 cggtgattgc cggtggtggc gatggcacca ttaatgaagt ttctacggcg ttgattcagt
    27481 gtgaggggga tgacataccc gcgctgggaa ttttgccatt aggaaccgcc aatgattttg
    27541 ccaccagtgt agggattcct gaggcactgg ataaggcgct gaaactggca attgccggta
    27601 atgccattgc gatagatatg gcgcaggtca acaaacaaac ctgttttatt aatatggcga
    27661 caggcggatt tgggacgcgt attaccacag aaacgccgga aaaattaaaa gccgcgctgg
    27721 gtggcgtctc ttacatcatt catggcttaa tgcgcatgga tactctgcaa ccggaccgtt
    27781 gtgaaatccg cggtgaaaac tttcactggc aaggtgacgc cctggtcatt ggtattggta
    27841 acgggcgtca ggccggtggc ggtcaacaat tgtgcccgaa cgcgttaatt aacgatggct
    27901 tgctgcaact gcgcattttt accggcgatg aaattcttcc ggctctcgta tcaaccttaa
    27961 aatctgacga agataacccg aatattatcg aaggcgcttc gtcgtggttt gatatacaag
    28021 ccccacacga aatcactttt aatcttgatg gcgaaccgtt gagtgggcaa aacttccata
    28081 ttgaaatact tccggcggcg ttgcgttgtc gattaccacc agattgtcca ttattgcgtt
    28141 aatcaaacta ttctctcttg aaccagggag aatccgtaat gccggatgcg gtgtaaacac
    28201 cttatccggc atacagaaca atactacacc gtaattaatt ttacccgtgt cttctgcaat
    28261 gccaatttat ccacctctga cacagcatca tcggtaacaa taatgtcaat tttttcgatt
    28321 ggcagtacct gattaaaacc gcgacgatta aatttcgatg aatcgagaac ggcaatcact
    28381 ttatttgcgg cggtgaccat cgccccgcta atcgaataac cttcattaaa ggtcgtaata
    28441 ccattaaccg catcaatacc atcagcaccg acaaacatta aatcggcatt aatatcctgc
    28501 aatgaacgct cggcaatgga accatgcatc gagcgcgttt tatggcggac cgtgccacca
    28561 cagacgacca gagtaatgtc tttattttcg gaaagggcaa acgctgccgg gagactgttg
    28621 gtaatcaccg tgatattttt agcggtcatt aatccttcgg cgataagcat tgtggtactt
    28681 ccgctgtcga gaataacggt catcccttca tggatcattt tgactgccgc ctgcgctatt
    28741 ctgttttttg gcgcgctggc gagctgaaat cgctctttaa acccgacttc ctgggtctcg
    28801 gtttcactat taccggacat tattttcgcc gcaccgccat gaaagcgcgt aaccacgcct
    28861 ttttgttcga gaaagcgcaa atcggcacgg attgtcgctt ccgaggcagc aaatactccc
    28921 gccagatcct gaacaagcac ggttccctgt tcattcacta attggatgat cttattcctt
    28981 cgctcgaatg aattcatcgt catttaccca tattcaattg tggctagtgt aaacgaaggg
    29041 ggaccattgg tgaatgatct gttttgcaca ataccgggcc agcatcacgc tggcccgcgg
    29101 tttcatggaa tgagcaacac tttgccctgc atggcattac gagcgatgtc acgcaccgcc
    29161 tgggtgaagc tttcaaagct tccacggtga gcgattaatg gctccaggct taacttacgt
    29221 tctgtcagca accggctcgc cgtttcccac tcctgccccg gccaagggct ggaatagttc
    29281 atccaactgc cgataaccgt cagctcttta cgcaatattt tgccaaacgt tgccgatgtt
    29341 aaatgcagat cctgatgcaa cgtgcccacc agcgccagtt gggcatgagg accggcaatc
    29401 tctaccgcca gttcgacagt ttgcggtacg ccagccgtct cgaggataag ctgattaaag
    29461 cgcagttcgc gtaaaacgct ctgcatttgc ggcgcgctca tttcgctact gttaaatgtt
    29521 tgcatcgcac cgaaagattt tgccagtgcc agtttttctg agctaatgtc gatcgccgtc
    29581 acactctttg ctcccagcgc gacagcgcac tgaatagcca gcaggccaat ggttccggca
    29641 ccaataataa taacgttttt attctcacaa ccttgcgcta aatgaaaagc atgcaggcca
    29701 acggtaatcg gctcaataaa agccccatcc tcaataggca tatccgtggg tagagcaaag
    29761 acattttttc gcttaacgac aatatattca gcaaatccac catcacgccg cgagccaata
    29821 aaatcatatt ttgcgcactg ggaataaaac cctttcaaac actctggaca agtaaaacag
    29881 ggtaataacg gcacacaggc aaccgcatcg ccagggtgta aatcatcaac accggatccc
    29941 accgcatcaa tatagccgct aaattcatgg cctaacgtta ttggataata atgtgcacca
    30001 tttttaaata tcctgggtaa atcggaacca cataagcccg agctggcaat ttttacccgc
    30061 acctcatcct gatgtttaat ttcaggaatg acgctttttg caacg
//
LOCUS       DAJTRC010000044        30012 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.044, whole genome shotgun sequence.
ACCESSION   DAJTRC010000044 DAJTRC010000000
VERSION     DAJTRC010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 30012)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 30012)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..30012
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.044"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            248..631
                     /gene="secE"
                     /locus_tag="OO357_002102"
     CDS             248..631
                     /gene="secE"
                     /locus_tag="OO357_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007665874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HCR5887049.1"
                     /translation="MSANTEAQGSGRGLEAMKWVVVVALLLAAIVGNYLYRDIMLPLR
                     ALAVVILIAAAGGVALLTTKGKATIAFAREARTEVRKVIWPTRQETLHTTLIVAAVTA
                     VMSLILWGLDGILVRLVSFITGLRF"
     gene            633..1178
                     /gene="nusG"
                     /locus_tag="OO357_002103"
     CDS             633..1178
                     /gene="nusG"
                     /locus_tag="OO357_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005280494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HCR5887050.1"
                     /translation="MSEAPKKRWYVVQAFSGFEGRVATSLREHIKLHNMEDLFGEVMV
                     PTEEVVEIRGGQRRKSERKFFPGYVLVQMVMNDASWHLVRSVPRVMGFIGGTSDRPAP
                     ISDKEVDAIMNRLQQVGDKPRPKTLFEPGEMVRVNDGPFADFNGVVEEVDYEKSRLKV
                     SVSIFGRATPVELDFSQVEKA"
     gene            1337..1765
                     /gene="rplK"
                     /locus_tag="OO357_002104"
     CDS             1337..1765
                     /gene="rplK"
                     /locus_tag="OO357_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006121495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HCR5887051.1"
                     /translation="MAKKVQAYVKLQVAAGMANPSPPVGPALGQQGVNIMEFCKAFNA
                     KTDSIEKGLPIPVVITVYADRSFTFVTKTPPAAVLLKKAAGIKSGSGKPNKDKVGKIS
                     RAQLQEIAQTKAADMTGADIEAMTRSIEGTARSMGLVVED"
     gene            1769..2473
                     /gene="rplA"
                     /locus_tag="OO357_002105"
     CDS             1769..2473
                     /gene="rplA"
                     /locus_tag="OO357_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HCR5887052.1"
                     /translation="MAKLTKRMRVIREKVDATKQYDINEAIALLKELATAKFVESVDV
                     AVNLGIDARKSDQNVRGATVLPHGTGRSVRVAVFTQGANAEAAKAAGAELVGMEDLAD
                     QIKKGEMNFDVVIASPDAMRVVGQLGQVLGPRGLMPNPKVGTVTPNVAEAVKNAKAGQ
                     VRYRNDKNGIIHTTIGKVDFDADKLKENLEALLVALKKAKPTQAKGVYIKKVSISTTM
                     GAGVAVDQAGLSASVN"
     gene            2886..3383
                     /gene="rplJ"
                     /locus_tag="OO357_002106"
     CDS             2886..3383
                     /gene="rplJ"
                     /locus_tag="OO357_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002438625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HCR5887053.1"
                     /translation="MALNLQDKQAIVAEVSEVAKGALSAVVADSRGVTVDKMTELRKA
                     GREAGVYMRVVRNTLLRRAVEGTPFECLKDAFVGPTLIAYSMEHPGAAARLFKEFAKA
                     NAKFEVKAAAFEGELIPASQIDRLATLPTYEEAIARLMATMKEASAGKLVRTLAAVRD
                     AKEAA"
     gene            3450..3815
                     /gene="rplL"
                     /locus_tag="OO357_002107"
     CDS             3450..3815
                     /gene="rplL"
                     /locus_tag="OO357_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005122872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HCR5887054.1"
                     /translation="MSITKDQIIEAVAAMSVMDVVELISAMEEKFGVSAAAAVAVAAG
                     PVEAAEEKTEFDVILKAAGANKVAVIKAVRGATGLGLKEAKDLVESAPAALKEGVSKD
                     DAEALKKALEEAGAEVEVK"
     gene            4135..8163
                     /gene="rpoB"
                     /locus_tag="OO357_002108"
     CDS             4135..8163
                     /gene="rpoB"
                     /locus_tag="OO357_002108"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004870488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HCR5887055.1"
                     /translation="MVYSYTEKKRIRKDFGKRPQVLDVPYLLSIQLDSFQKFIEQDPE
                     GQYGLEAAFRSVFPIQSYSGNSELQYVSYRLGEPVFDVQECQIRGVTYSAPLRVKLRL
                     VIYEREAPEGTVKDIKEQEVYMGEIPLMTDNGTFVINGTERVIVSQLHRSPGVFFDSD
                     KGKTHSSGKVLYNARIIPYRGSWLDFEFDPKDNLFVRIDRRRKLPATIILRALNYTTE
                     QILDLFFEKVIFEIRDNKLQMELVPERLRGETASFDIEANGKVYVEKGRRITARHIRQ
                     LEKDDVKLIEVPVEYIAGKVVAKDYIDESTGELICAANMELSLDLLAKLSQSGHKRIE
                     TLFTNDLDHGPYISETLRVDPTNDRLSALVEIYRMMRPGEPPTREAAESLFENLFFSE
                     DRYDLSAVGRMKFNRSLLREEIEGSGILSKDDIIDVMKKLIDIRNGKGEVDDIDHLGN
                     RRIRSVGEMAENQFRVGLVRVERAVKERLSLGDLDTLMPQDMINAKPISAAVKEFFGS
                     SQLSQFMDQNNPLSEITHKRRISALGPGGLTRERAGFEVRDVHPTHYGRVCPIETPEG
                     PNIGLINSLSVYAQTNEYGFLETPYRKVTDGVVTDEIHYLSAIEEGNYVIAQANSNLD
                     EEGHFVEDLVTCRSKGESSLFSRDQVDYMDVSTQQVVSVGASLIPFLEHDDANRALMG
                     ANMQRQAVPTLRADKPLVGTGMERAVAVDSGVTAVAKRGGVVQYVDASRIVIKVNEDE
                     MYPGEAGIDIYNLTKYTRSNQNTCINQMPCVSLGEPVERGDVLADGPSTDLGELALGQ
                     NMRVAFMPWNGYNFEDSILVSERVVQEDRFTTIHIQELACVSRDTKLGPEEITADIPN
                     VGEAALSKLDESGIVYIGAEVTGGDILVGKVTPKGETQLTPEEKLLRAIFGEKASDVK
                     DSSLRVPNGVSGTVIDVQVFTRDGVEKDKRALEIEEMQLKQAKKDLSEELQILEAGLF
                     SRIRAVLVAGGVEAEKLDKLPRDRWLELGLTDEEKQNQLEQLAEQYDELKHEFEKKLE
                     AKRRKITQGDDLAPGVLKIVKVYLAVKRRIQPGDKMAGRHGNKGVISKINPIEDMPYD
                     ENGTPVDIVLNPLGVPSRMNIGQILETHLGMAAKGIGDKINAMLKQQQEVAKLREFIQ
                     RAYDLGADVRQKVDLSTFSDEEVMRLAENLRKGMPIATPVFDGAKEAEIKELLKLGDL
                     PTSGQIRLYDGRTGEQFERPVTVGYMYMLKLNHLVDDKMHARSTGSYSLVTQQPLGGK
                     AQFGGQRFGEMEVWALEAYGAAYTLQEMLTVKSDDVNGRTKMYKNIVDGNHQMEPGMP
                     ESFNVLLKEIRSLGINIELEDE"
     gene            8240..12463
                     /gene="rpoC"
                     /locus_tag="OO357_002109"
     CDS             8240..12463
                     /gene="rpoC"
                     /locus_tag="OO357_002109"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HCR5887056.1"
                     /translation="MKDLLKFLKAQTKTEEFDAIKIALASPDMIRSWSFGEVKKPETI
                     NYRTFKPERDGLFCARIFGPVKDYECLCGKYKRLKHRGVICEKCGVEVTQTKVRRERM
                     GHIELASPTAHIWFLKSLPSRIGLLLDMPLRDIERVLYFESYVVIEGGMTNLERQQIL
                     TEEQYLDALEEFGDEFDAKMGAEAIQALLKSMDLEQECEQLREELNETNSETKRKKLT
                     KRIKLLEAFVQSGNKPEWMILTVLPVLPPDLRPLVPLDGGRFATSDLNDLYRRVINRN
                     NRLKRLLDLAAPDIIVRNEKRMLQEAVDALLDNGRRGRAITGSNKRPLKSLADMIKGK
                     QGRFRQNLLGKRVDYSGRSVITVGPYLRLHQCGLPKKMALELFKPFIYGKLELRGLAT
                     TIKAAKKMVEREEAVVWDILDEVIREHPVLLNRAPTLHRLGIQAFEPVLIEGKAIQLH
                     PLVCAAYNADFDGDQMAVHVPLTLEAQLEARALMMSTNNILSPANGEPIIVPSQDVVL
                     GLYYMTRDCVNAKGEGMVLTGPKEAERLYRSGLASLHARVKVRITEYEKDANGELVAK
                     TSLKDTTVGRAILWMIVPKGLPYSIVNQALGKKAISKMLNTCYRILGLKPTVIFADQI
                     MYTGFAYAARSGASVGIDDMVIPEKKHEIISEAEAEVAEIQEQFQSGLVTAGERYNKV
                     IDIWAAANDRVSKAMMDNLQTETVINRDGQEEKQVSFNSIYMMADSGARGSAAQIRQL
                     AGMRGLMAKPDGSIIETPITANFREGLNVLQYFISTHGARKGLADTALKTANSGYLTR
                     RLVDVAQDLVVTEDDCGTHEGIMMTPVIEGGDVKEPLRDRVLGRVTAEDVLKPGTADI
                     LVPRNTLLHEQWCDLLEENSVDAVKVRSVVSCDTDFGVCAHCYGRDLARGHIINKGEA
                     IGVIAAQSIGEPGTQLTMRTFHIGGAASRAAAESSIQVKNKGSIKLSNVKSVVNSSGK
                     LVITSRNTELKLIDEFGRTKESYKVPYGAVLAKGDGEQVAGGETVANWDPHTMPVITE
                     VSGFVRFTDMIDGQTITRQTDELTGLSSLVVLDSAERTAGGKDLRPALKIVDAQGNDV
                     LIPGTDMPAQYFLPGKAIVQLEDGVQISSGDTLARIPQESGGTKDITGGLPRVADLFE
                     ARRPKEPAILAEISGIVSFGKETKGKRRLVITPVDGSDPYEEMIPKWRQLNVFEGERV
                     ERGDVISDGPEAPHDILRLRGVHAVTRYIVNEVQDVYRLQGVKINDKHIEVIVRQMLR
                     KATIVNAGSSDFLEGEQVEYSRVKIANRELEANGKVGATYSRDLLGITKASLATESFI
                     SAASFQETTRVLTEAAVAGKRDELRGLKENVIVGRLIPAGTGYAYHQDRMRRRAAGEA
                     PAAPQVTAEDASASLAELLNAGLGGSDNE"
     gene            complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO357_002110"
     CDS             complement(12705..13838)
                     /gene="thiH"
                     /locus_tag="OO357_002110"
                     /EC_number="4.1.99.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminoacetate synthase ThiH"
                     /protein_id="HCR5887057.1"
                     /translation="MKTFSNRWRQLDWDDIRLRINGKTAADVERALNASQLTRDDMMA
                     LLSPAASGYLEQLAQQAQRLTRQRFGNTVSFYVPLYLSNLCANDCTYCGFSMSNRIKR
                     KTLDEADIARESAAIREMGFEHLLLVTGEHQAKVGMDYFRRHLPALREQFSSLQMEVQ
                     PLAETEYAELKQLGLDGVMVYQETYHEATYTRHHLKGKKQDFFWRLETPDRLGRAGID
                     KIGLGALIGLSDNWRVDCYMVAEHLLWLQQHYWQSRYSVSFPRLRPCTGGIEPASIMD
                     ERQLVQTICAFRLLAPEIELSLSTRESPWFRDRVIPLAINNVSAFSKTQPGGYADNHP
                     ELEQFSPHDDRRPEAVAAVLTAQGLQPVWKDWDSYLGRASQRL"
     gene            complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO357_002111"
     CDS             complement(13835..14605)
                     /gene="thiG"
                     /locus_tag="OO357_002111"
                     /EC_number="2.8.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418418.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HCR5887058.1"
                     /translation="MLRIADKTFDSHLFTGTGKFASSQLMVEAIRASGSQLVTLAMKR
                     VDLRQHNDAILEPLIAAGVALLPNTSGAKTAEEAIFAAHLAREALGTNWLKLEIHPDA
                     RWLLPDPIETLKAAETLVQQGFVVLPYCGADPVLCKRLEEVGCAAVMPLGAPIGSNQG
                     LETRAMLEIIIQQATVPVVVDAGIGVPSHATQALEMGADAVLVNTAIAVADDPVNMAK
                     AFRLAVEVGLLARQSGPGSRSYFAHATSPLTGFLEASA"
     gene            complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO357_002112"
     CDS             complement(14607..14807)
                     /gene="thiS"
                     /locus_tag="OO357_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfur carrier protein ThiS"
                     /protein_id="HCR5887059.1"
                     /translation="MQILFNDQPMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPRE
                     QWTQHIVQDGDQILLFQVIAGG"
     gene            complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO357_002113"
     CDS             complement(14791..15546)
                     /gene="thiF"
                     /locus_tag="OO357_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709786.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiazole biosynthesis adenylyltransferase ThiF"
                     /protein_id="HCR5887060.1"
                     /translation="MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAA
                     LYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDINSPKSQVSQQRLTQLNPDIQLTA
                     LQQRLTGEALKDAVAQADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMV
                     LTPPWEQGCYRCLWPDNQQPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGE
                     LRLFDGKSSQWRSLALRRASGCPVCGGSNADPV"
     gene            complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO357_002114"
     CDS             complement(15539..16174)
                     /gene="thiE"
                     /locus_tag="OO357_002114"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709787.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HCR5887061.1"
                     /translation="MYQPDFPPVPFRLGLYPVVDSVQWIERLLDAGVRTLQLRIKDRR
                     DEEVEADVVAAIALGRRYNARLFINDYWRLAIKHQAYGVHLGQEDLQATDLNAIRAAG
                     LRLGVSTHDDMEIDVALAARPSYIALGHVFPTQTKQMPSAPQGLEQLARHVERLADYP
                     TVAIGGISLARAPAVIATGVGSIAVVSAITQAADWRLATAQLLEIAGVGDE"
     gene            complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO357_002115"
     CDS             complement(16174..18069)
                     /gene="thiC"
                     /locus_tag="OO357_002115"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HCR5887062.1"
                     /translation="MSATKLTRREQRARAQHFIDTLEGTAFPNSKRIYITGTHPGVRV
                     PMREIQLSPTLIGGSKEQPQYEENEAIPVYDTSGPYGDPQIAINVQQGLAKLRQPWID
                     ARADTEELTVRSSDYTRTRLADDGLDELRFSGLLTPKRAKTGRRVTQLHYARQGIITP
                     EMEFIAIRENMGRERIRSEVLRHQHPGMSFGAHLPENITAEFVRDEVAAGRAIIPANI
                     NHPESEPMIIGRNFLVKVNANIGNSAVTSSIEEEVEKLVWSTRWGADTVMDLSTGRYI
                     HETREWILRNSPVPIGTVPIYQALEKVNGIAEDLTWEAFRDTLLEQAEQGVDYFTIHA
                     GVLLRYVPMTAKRLTGIVSRGGSIMAKWCLSHHQENFLYQHFREICEICAAYDVSLSL
                     GDGLRPGSIQDANDEAQFAELHTLGELTKIAWEYDVQVMIEGPGHVPMQMIRRNMTEE
                     LEHCHEAPFYTLGPLTTDIAPGYDHFTSGIGAAMIGWFGCAMLCYVTPKEHLGLPNKE
                     DVKQGLITYKIAAHAADLAKGHPGAQIRDNAMSKARFEFRWEDQFNLALDPFTARAYH
                     DETLPQESGKVAHFCSMCGPKFCSMKISQEVRDYAAAQTIEVGMADMSENFRARGGEI
                     YLHKEEA"
     regulatory      complement(18150..18250)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO357_002116"
     CDS             complement(18303..18779)
                     /gene="rsd"
                     /locus_tag="OO357_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma D regulator"
                     /protein_id="HCR5887063.1"
                     /translation="MLNQLDNLTERVRGSNKLVDRWLHVRKHLLVAYYNLVGIKPGKE
                     SYMRLNEKALDDFCQSLVDYLSAGHFSIYERILHKLEGNGQLARAAKIWPQLEANTQQ
                     IMDYYDSSLETAIDHDNYLEFQQVLSDIGEALEARFVLEDKLILLVLDAARVKHPA"
     gene            18874..19647
                     /gene="nudC"
                     /locus_tag="OO357_002117"
     CDS             18874..19647
                     /gene="nudC"
                     /locus_tag="OO357_002117"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HCR5887064.1"
                     /translation="MDRIIEKLDHGWWVVSHEQKLWLPKGELPYGEAANFDLVGQRAL
                     QIGEWQGEPVWLIQQQRRHDMGSVRQVIDLDVGLFQLAGRGVQLAEFYRSHKYCGYCG
                     HEMYPSKTEWAMLCSHCRERYYPQIAPCIIVAIRRDDSLLLAQHTRHRNGVHTVLAGF
                     VEVGETLEQAVAREVMEESGIKVKNLRYVTSQPWPFPQSLMTAFMAEYDSGDIVIDPK
                     ELLEANWYRYDDLPLLPPPGTVARRLIEDTVAMCRAEYE"
     gene            19687..20751
                     /gene="hemE"
                     /locus_tag="OO357_002118"
     CDS             19687..20751
                     /gene="hemE"
                     /locus_tag="OO357_002118"
                     /EC_number="4.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase"
                     /protein_id="HCR5887065.1"
                     /translation="MTELKNDRYLRALLRQPVDVTPVWMMRQAGRYLPEYKATRAQAG
                     DFMSLCKNAELACEVTLQPLRRYPLDAAILFSDILTVPDAMGLGLYFEAGEGPRFTSP
                     VTCKADVDKLPIPDPEDELGYVMNAVRTIRHELKGEVPLIGFSGSPWTLATYMVEGGS
                     SKAFTVIKKMMYADPQALHALLDKLAKSVTLYLNAQIKAGAQAVMIFDTWGGVLTGRD
                     YQQFSLYYMHKIVDGLLRENDGRRVPVTLFTKGGGQWLEAMAETGCDALGLDWTTDIA
                     DARRRVGNKVALQGNMDPSMLYAPPARIEEEVATILAGFGHGEGHVFNLGHGIHQDVP
                     PEHAGVFVEAVHRLSEQYHR"
     gene            20908..21579
                     /gene="nfi"
                     /locus_tag="OO357_002119"
     CDS             20908..21579
                     /gene="nfi"
                     /locus_tag="OO357_002119"
                     /EC_number="3.1.21.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease V"
                     /protein_id="HCR5887066.1"
                     /translation="MDLASLRAQQIELASSVICEDRLDKDPPDLIAGADVGFEQGGEV
                     TRAAMVLLKYPSLELVEYKVARIATTMPYIPGFLSFREYPALLAAWEMLSQKPDLVFV
                     DGHGISHPRRLGVASHFGLLVDVPTIGVAKKRLCGKFEPLSSKPGALAPLMDKGEQLA
                     WVWRSKARCNPLFIATGHRVSVDSALAWVQRCMKGYRLPEPTRWADAVASERPAFVRY
                     TANQP"
     gene            21622..22212
                     /locus_tag="OO357_002120"
     CDS             21622..22212
                     /locus_tag="OO357_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjaG family protein"
                     /protein_id="HCR5887067.1"
                     /translation="MLQNPIHLRLERLESWQHVTFMACLCERMYPNYAMFCQQTGFGD
                     GQIYRRILDLIWETLTVKDAKVNFDSQLEKFEEAIPSADDFDLYGVYPAIDACVALSE
                     LVHSRLSGETLEHAVEVSKTSITTVAMLEMTQAGREMGDEELKENPAVEQEWDIQWEI
                     FRLLAECEERDIELIKGLRADLRESGESNIGIIFQQ"
     gene            22399..22671
                     /gene="hupA"
                     /locus_tag="OO357_002121"
     CDS             22399..22671
                     /gene="hupA"
                     /locus_tag="OO357_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017460010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-alpha"
                     /protein_id="HCR5887068.1"
                     /translation="MNKTQLIDVIAEKAELSKTQAKAALESTLAAITESLKEGDAVQL
                     VGFGTFKVNHRAERTGRNPQTGKEIKIAAANVPAFVSGKALKDAVK"
     gene            22684..23379
                     /locus_tag="OO357_002122"
     CDS             22684..23379
                     /locus_tag="OO357_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1481 domain-containing protein"
                     /protein_id="HCR5887069.1"
                     /translation="MNSFNEGVVSPLLSFWRRSLMLAGALFLTACSHNSSLPPFTASG
                     FAEDQGAVRIWRKDSGDNVHLLAVFSPWRSGDTTTREYRWQGDNLTLININVYSKPPV
                     NIRARFDDRGDLSFMQRESDGEKQQLSNDQIDLYRYRADQIRQISDALRQGRVVLRQG
                     RWHAMEQTVTTCEGQTIKPDLDSQAIAHIERRQSRSSVDVSVAWLEAPEGSQLLLVAN
                     SDFCRWQPNEKTF"
     gene            complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO357_002123"
     CDS             complement(23381..23806)
                     /gene="zraP"
                     /locus_tag="OO357_002123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc resistance sensor/chaperone ZraP"
                     /protein_id="HCR5887070.1"
                     /translation="MKRNTKIALVMMALSAMAMGSTSAFAHGGHGMWQQNAAPLTSEQ
                     QTAWQKIHNDFYAQSSALQQQLVTKRYEYNALLAANPPDSSKINAVAKEMENLRQSLD
                     ELRVKRDIAMAEAGIPRGAGMGMGYGGCGGGGHMGMGHW"
     gene            24044..25420
                     /gene="zraS"
                     /locus_tag="OO357_002124"
     CDS             24044..25420
                     /gene="zraS"
                     /locus_tag="OO357_002124"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     ZraS"
                     /protein_id="HCR5887071.1"
                     /translation="MRFMQRSKDSLAKWLSAILPVVIVGLVGLFAVTVIRDYGRETAA
                     ARQTLLEKGSVLIRALESGSRVGMGMRMHHAQQQALLEEMAGQPGVRWFAVTDEQGTI
                     VMHSNSGMVGKQLYSPQEMQQLHPGDEEAWRRIDSADGEPVLEIYRQFQPMFAAGMYR
                     MRHMQQYAATPQAIFIAFDASNIVSAEDREQRNTLIILFALATVLLASVLSFFWYRRY
                     LRSRQLLQDEMKRKEKLVALGHLAAGVAHEIRNPLSSIKGLAKYFAERAPAGGEAHQL
                     AQVMAKEADRLNRVVSELLELVKPTHLALQAVDLNTLINHSLQLVSQDANSREIQLRF
                     TANDTLPEIQADPDRLTQVLLNLYLNAIQAIGQHGVISVTASESGAGVKISVTDSGKG
                     IAADQLEAIFTPYFTTKAEGTGLGLAVVHNIVEQHGGTIQVASQEGKGSTFTLWLPVN
                     ITRKDPQG"
     gene            25417..26742
                     /gene="zraR"
                     /locus_tag="OO357_002125"
     CDS             25417..26742
                     /gene="zraR"
                     /locus_tag="OO357_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54-dependent response regulator
                     transcription factor ZraR"
                     /protein_id="HCR5887072.1"
                     /translation="MTHDNIDILVVDDDISHCTILQALLRGWGYNVALANSGRQALEQ
                     VREQVFDLVLCDVRMAEMDGIATLKEIKALNPAIPVLIMTAYSSVETAVEALKTGAQD
                     YLIKPLDFDNLQATLEKALAHTHSIDAETPAVTASQFGMVGKSPAMQHLLSEIALVAP
                     SEATVLIHGDSGTGKELVARAIHASSARSEKPLVTLNCAALNESLLESELFGHEKGAF
                     TGADKRREGRFVEADGGTLFLDEIGDISPMMQVRLLRAIQEREVQRVGSNQTISVDVR
                     LIAATHRDLAAEVNAGRFRQDLYYRLNVVAIEVPSLRQRREDIPLLAGHFLQRFAERN
                     RKAVKGFTPQAMDLLIHYDWPGNIRELENAVERAVVLLTGEYISERELPLAIASTPIP
                     LGQSQDIQPLVEVEKEVILAALEKTGGNKTEAARQLGITRKTLLAKLSR"
     gene            complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO357_002126"
     CDS             complement(26739..28028)
                     /gene="purD"
                     /locus_tag="OO357_002126"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HCR5887073.1"
                     /translation="MKVLVIGNGGREHALAWKAAQSPLVETVFAAPGNAGTALEPALQ
                     NVAIGVTDIPALLDFAQNEKIDLTIVGPEAPLVKGVVDTFRAAGLKIFGPTAGAAQLE
                     GSKAFTKDFLARHKIPTAEYQNFTEVEPALVYLREKGAPIVIKADGLAAGKGVIVAMT
                     LEEAEAAVHDMLAGNAFGDAGHRIVIEEFLDGEEASFIVMVDGEHVLPMATSQDHKRV
                     GDKDNGPNTGGMGAYSPAPVVTDEVHQRTMERIIWPTVKGMAAEGNTYTGFLYAGLMI
                     DKQGNPKVIEFNCRFGDPETQPIMLRMKSDLVELCLAACEGKLDEKTSEWDERASLSV
                     VMAAGGYPGDYRTGDVIHGLPLEEVADGKVFHAGTKLADDEQVVTSGGRVLCVTALGH
                     TVAEAQKRAYALMTDIHWDDCFCRKDIGWRAIEREQN"
     gene            complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO357_002127"
     CDS             complement(28040..29629)
                     /gene="purH"
                     /locus_tag="OO357_002127"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HCR5887074.1"
                     /translation="MQQRRPVRRALLSVSDKAGIVEFAQALSARGVELLSTGGTARLL
                     AEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGILGRRGQDDAIMEEHQIQPIDMVV
                     VNLYPFAQTVAREGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
                     DDNEGSLTLATRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNL
                     NFIKKQDMRYGENSHQQAAFYIEENVKEASVATATQVQGKALSYNNIADTDAALECVK
                     EFAEPACVIVKHANPCGVAIGNSILDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAI
                     ISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWGERVPGLDFKRVNGGLLVQDRD
                     LGMVGAEELRVVTQRQPTEQELRDALFCWKVAKFVKSNAIVYAKNNMTIGIGAGQMSR
                     VYSAKIAGIKAADEGLEVKGSSMASDAFFPFRDGIDAAAAAGVTCVIQPGGSIRDDEV
                     IAAADEHGIAMLFTDMRHFRH"
BASE COUNT         7027 a         7824 c         8255 g         6906 t
ORIGIN      
        1 gcaaaagttc tgagctaatt gccgataaca tttgacgcaa tgcgcactaa aagggcatca
       61 tttgatgccc tttttgcacg ctttcgtacc agaacctggc tcatcagtga ttttctttgt
      121 cataatcatt gctgagacag gctctgttga gggcgtataa tccgaaaagc taatacgcgt
      181 ttcgatttgg tttgcctcgc gatcgcgggg tgaaaatgtt tgtagaaaac ttctgacagg
      241 ttggtttatg agtgcgaata ccgaagctca aggaagcggg cgcggcctgg aagcgatgaa
      301 gtgggtcgtt gtggtggcat tgctcctggc ggcgattgtc ggcaactacc tttatcgcga
      361 cattatgctg ccgctgcgtg cgctggccgt agtaattctg attgctgcag cgggtggtgt
      421 cgcgctgtta acgacaaaag gtaaagctac cattgctttt gcccgtgaag cgcgtaccga
      481 agtccgtaag gtcatttggc cgactcgcca ggaaacattg cacaccacgc tgattgtggc
      541 tgcggttacc gcagtaatgt cactgatcct gtggggactg gatggtattc tggttcgcct
      601 ggtatccttt atcactggcc tgaggttctg agatgtctga agctcctaaa aagcgctggt
      661 acgtcgttca ggcgttttcc ggttttgaag gccgcgtagc aacgtcgctg cgtgagcata
      721 tcaaattaca caacatggaa gatttgtttg gtgaagtcat ggtaccaacc gaagaagtgg
      781 ttgaaatccg tggcggtcag cgtcgcaaaa gcgaacgtaa attcttccct ggctacgtcc
      841 tcgttcagat ggtgatgaac gacgcgagct ggcacttggt gcgcagcgta ccgcgtgtga
      901 tgggcttcat cggcggtact tccgatcgtc ctgcgccaat cagcgataaa gaagtcgatg
      961 cgattatgaa ccgcctgcag caggttggtg ataagccgcg tccgaaaacg ctgtttgaac
     1021 cgggtgaaat ggtccgtgtt aatgatggtc cgttcgctga cttcaacggt gttgttgaag
     1081 aagtggatta cgagaaatct cgtctgaaag tgtctgtttc tatcttcggt cgtgcgaccc
     1141 cggtagagct ggacttcagc caggttgaaa aagcctaacc cagcgatcaa aaaagcggcg
     1201 atttaatcgt tgcacaaggc gtgagattgg aatacaattt cgcgcctttt gtttttatgg
     1261 gccttgcccg taaaacgatt ttttatatca cggggagcct ctcagaggcg ttattaccca
     1321 acttgaggaa tttataatgg ctaagaaagt acaagcctat gtcaagctgc aggttgcagc
     1381 tggtatggct aacccgagtc cgccagtagg tccggctctg ggtcagcagg gcgtaaacat
     1441 catggaattc tgcaaagcgt tcaacgcaaa aactgattcc atcgaaaaag gtctgccgat
     1501 tccggtagta atcaccgttt acgctgaccg ttctttcact ttcgttacca agaccccgcc
     1561 ggcagcagtt ctgctgaaaa aagcggcagg tatcaagtct ggttccggta agccgaacaa
     1621 agacaaagtg ggtaaaattt cccgcgctca gctgcaggaa atcgcgcaga ccaaagctgc
     1681 cgacatgact ggtgccgaca ttgaagcgat gactcgctcc atcgaaggta ctgcacgttc
     1741 catgggcctg gtagtggagg actaagaaat ggctaaactg accaagcgca tgcgtgttat
     1801 ccgtgagaaa gttgatgcaa ccaaacagta cgacatcaac gaagctatcg cactgctgaa
     1861 agagctggcg actgctaaat tcgtagaaag cgtggacgta gctgttaacc tcggcatcga
     1921 cgctcgtaaa tctgaccaga acgtacgtgg tgcaactgta ctgccgcacg gtactggccg
     1981 ttccgttcgc gtagccgtat ttacccaagg tgcaaacgct gaagctgcta aagctgcagg
     2041 cgcagaactg gtaggtatgg aagatctggc tgaccagatc aagaaaggcg aaatgaactt
     2101 tgacgttgtt attgcttctc cggatgcaat gcgcgttgtt ggccagctgg gccaggttct
     2161 gggtccgcgt ggcctgatgc caaacccgaa agtgggtact gtaacaccga acgttgctga
     2221 agcggttaaa aacgctaaag ctggccaggt tcgttaccgt aacgacaaaa acggcatcat
     2281 ccacaccacc atcggtaaag tggactttga cgctgacaaa ctgaaagaaa acctggaagc
     2341 tctgctggtt gcgctgaaaa aagcaaaacc gactcaggcg aaaggcgtgt acatcaagaa
     2401 agttagcatc tccaccacca tgggtgcagg tgttgcagtt gaccaggctg gcctgagcgc
     2461 ttctgtaaac taatgccttt acgtgggcgg tgattttgtc tacaatctta cccccacgta
     2521 taatgcttaa tgcagacgta tatccgagat attcgggttg tggcaaggcg gcaactgagt
     2581 gagtcgccaa gagcatagct aactatgtga ctggtgcgaa tgaaggaagc caacgccgcc
     2641 acaagttgaa tagcgacgga tagaaaagat ttgttcgttg gagcctggcc tatccaggcc
     2701 tccgtcgaag accgcaggag tttcgcaaga aacttaatcc cctgcgtaga cggtgacaga
     2761 acgctaagat tattctttta tattctggct tgtttctgct caccgtaatt aagacgctct
     2821 ctccgtttgg aggagtgaag tgagttccag agattttctc tggcaaacat ccaggagcaa
     2881 agctaatggc tttaaatctt caagacaaac aagcgattgt tgctgaagtc agcgaagtag
     2941 ccaaaggcgc gctgtctgca gtagttgcgg attcccgtgg cgtaactgta gataaaatga
     3001 ctgaactgcg taaagcaggt cgcgaagctg gcgtatacat gcgtgttgtt cgtaacaccc
     3061 tgctgcgccg tgctgttgaa ggtactccgt tcgagtgcct gaaagacgcg tttgttggtc
     3121 cgaccctgat tgcatactct atggaacacc cgggcgctgc tgctcgtctg ttcaaagagt
     3181 tcgcgaaagc gaatgcaaaa tttgaggtca aagccgctgc ctttgaaggt gagctgatcc
     3241 cggcgtctca gatcgaccgc ctggcaactc tgccgaccta cgaagaagca attgcacgcc
     3301 tgatggcaac catgaaagaa gcttcggctg gcaaactggt tcgtactctg gctgctgtac
     3361 gcgatgcgaa agaagctgct taatcgcagt tatcttttta acgcattcgc ttacgtataa
     3421 acttattctg atattcagga acaatttaaa tgtctatcac taaagatcaa atcattgaag
     3481 cagttgcagc tatgtctgta atggacgttg tagaactgat ctctgcaatg gaagaaaaat
     3541 tcggtgtttc cgctgctgct gctgtagctg tagctgctgg cccggttgaa gctgctgaag
     3601 aaaaaactga attcgacgta attctgaaag ctgctggcgc taacaaagtt gctgttatca
     3661 aagcagtacg tggcgcaact ggcctgggtc tgaaagaagc taaagacctg gtagaatctg
     3721 caccggctgc tctgaaagaa ggcgtgagca aagacgacgc agaagcactg aaaaaagctc
     3781 tggaagaagc tggcgctgaa gttgaagtta aataagccaa cccttccggt tgcagcctga
     3841 gaaatcaggc tgatggctgg tgacttttta gtcaccagcc tttttgcgct gtaaggcgcc
     3901 agtagcgttt cacactgttt gactactgct gtgcctttca atgcttgttt ctatcgacga
     3961 cttaatatac tgcgacagga cgtccgttct gtgtaaatcg caatgaaatg gtttaagcgt
     4021 gatagcaaca ggcattgcgg aaagtgttcc attttccggt caacaaaata gtgttgcaca
     4081 aactgtccgc tcaatggaca gatgggtcga cttgtcagcg agctgaggaa ccctatggtt
     4141 tactcctata ccgagaaaaa acgtattcgt aaggattttg gtaaacgtcc acaagttctg
     4201 gatgtacctt atctcctttc tatccagctt gactcgtttc agaaatttat cgagcaagat
     4261 cctgaagggc agtatggtct ggaagctgct ttccgttccg tattcccgat tcagagctac
     4321 agcggtaatt ccgagctgca atacgtcagc taccgccttg gcgaaccggt gtttgacgtc
     4381 caggaatgtc aaatccgtgg cgtgacctat tccgcaccgc tgcgcgttaa actgcgtctg
     4441 gtgatctatg agcgcgaagc gccggaaggc accgtaaaag acattaaaga acaagaagtc
     4501 tacatgggcg aaattccgct catgacagac aacggtacct ttgttatcaa cggtactgag
     4561 cgtgttatcg tttcccagct gcaccgtagt ccgggcgtct tctttgactc cgacaaaggt
     4621 aaaacccact cttcgggtaa agtgctgtat aacgcgcgca tcatccctta ccgtggttcc
     4681 tggctggact tcgaattcga tccgaaggac aacctgttcg tacgtatcga ccgtcgccgt
     4741 aaactgcctg cgaccatcat tctgcgcgcc ctgaactaca ccacagagca gatcctcgac
     4801 ctgttctttg aaaaagttat ctttgaaatc cgtgataaca agctgcagat ggaactggtg
     4861 ccggaacgcc tgcgtggtga aaccgcatct tttgacatcg aagctaacgg taaagtgtac
     4921 gtagaaaaag gccgccgtat cactgcgcgc cacattcgcc agctggaaaa agacgacgtc
     4981 aaactgattg aagtcccggt tgagtacatc gcaggtaaag tggttgctaa agactatatt
     5041 gatgagtcta ccggcgagct gatctgcgca gcgaacatgg agctgagcct ggatctgctg
     5101 gctaagctga gccagtctgg tcacaagcgt atcgaaacgc tgttcaccaa cgatctggat
     5161 cacggcccat atatctctga aaccttacgt gtcgacccaa ctaacgaccg tctgagcgca
     5221 ctggtagaaa tctaccgcat gatgcgccct ggcgagccgc cgactcgtga agcagctgaa
     5281 agcctgttcg agaacctgtt cttctccgaa gaccgttatg acttgtctgc ggttggtcgt
     5341 atgaagttca accgttctct gctgcgcgaa gaaatcgaag gttccggtat tctgagcaaa
     5401 gacgacatca ttgatgttat gaaaaagctc atcgatatcc gtaacggtaa aggcgaagtc
     5461 gatgatatcg accacctcgg caaccgtcgt atccgttccg ttggcgaaat ggcggaaaac
     5521 cagttccgcg ttggcctggt acgtgtagag cgtgcggtga aagagcgtct gtctctgggc
     5581 gatctggata ccctgatgcc tcaggatatg atcaacgcca agccgatttc cgcagcagtg
     5641 aaagagttct tcggttccag ccagctgtct cagtttatgg accagaacaa cccgctgtct
     5701 gagattacgc acaaacgtcg tatctccgca ctcggcccag gcggtctgac ccgtgaacgt
     5761 gcaggcttcg aagttcgaga cgtacacccg actcactacg gtcgcgtatg tccaatcgaa
     5821 acccctgaag gtccgaacat cggtctgatc aactctctgt ccgtgtacgc acagactaac
     5881 gaatacggct tccttgagac tccgtatcgt aaagtgaccg acggtgttgt aactgacgaa
     5941 attcactacc tgtctgctat cgaagaaggc aactacgtta tcgcccaggc gaactccaac
     6001 ctggatgaag aaggccactt cgtagaagac ctggtaactt gccgtagcaa aggcgaatcc
     6061 agcttgttca gccgcgacca ggttgactac atggacgtat ccacccagca ggtggtatcc
     6121 gtcggtgcgt ccctgatccc gttcctggaa cacgatgacg ccaaccgtgc attgatgggt
     6181 gcgaatatgc aacgtcaggc cgttccgact ctgcgcgctg ataagccgct ggttggtact
     6241 ggtatggaac gtgctgttgc cgttgactcc ggtgtaactg cggtagctaa acgtggtggt
     6301 gtcgttcagt acgtggatgc ttcccgtatc gttatcaaag ttaacgaaga cgagatgtat
     6361 ccgggtgaag caggtatcga catctacaac ctgaccaaat acacccgttc taaccagaac
     6421 acctgtatca accagatgcc gtgtgtgtct ctgggtgaac cggttgaacg tggcgacgtg
     6481 ctggcagacg gtccgtccac cgacctcggt gaactggcgc ttggtcagaa catgcgcgta
     6541 gcgttcatgc cgtggaatgg ttacaacttc gaagactcca tcctcgtatc cgagcgtgtt
     6601 gttcaggaag accgtttcac caccatccac attcaggaac tggcgtgtgt gtcccgtgac
     6661 accaagctgg gtccggaaga gatcaccgct gacatcccga acgtgggtga agctgcgctc
     6721 tccaaactgg atgaatccgg tatcgtttac attggtgcgg aagtgaccgg tggcgacatt
     6781 ctggttggta aggtaacgcc gaaaggtgaa actcagctga ccccagaaga aaaactgctg
     6841 cgtgcgatct tcggtgagaa agcctctgac gttaaagact cttctctgcg cgtaccaaac
     6901 ggtgtatccg gtacggttat cgacgttcag gtctttactc gcgatggcgt agaaaaagac
     6961 aaacgtgcgc tggaaatcga agaaatgcag ctcaaacagg cgaagaaaga cctgtctgaa
     7021 gaactgcaga tcctcgaagc gggtctgttc agccgtatcc gtgctgtgct ggtagccggt
     7081 ggcgttgaag ctgagaagct cgacaaactg ccgcgcgatc gctggctgga gctgggcctg
     7141 acagacgaag agaaacaaaa tcagctggaa cagctggctg agcagtatga cgaactgaaa
     7201 cacgagttcg agaagaaact cgaagcgaaa cgccgcaaaa tcacccaggg cgacgatctg
     7261 gcaccgggcg tgctgaagat tgttaaggta tatctggcgg ttaaacgccg tatccagcct
     7321 ggtgacaaga tggcaggtcg tcacggtaac aagggtgtaa tttctaagat caacccgatc
     7381 gaagatatgc cttacgatga aaacggtacg ccggtagaca tcgtactgaa cccgctgggc
     7441 gtaccgtctc gtatgaacat cggtcagatc ctcgaaaccc acctgggtat ggctgcgaaa
     7501 ggtatcggcg acaagatcaa cgccatgctg aaacagcagc aagaagtcgc gaaactgcgc
     7561 gaattcatcc agcgtgcgta cgatctgggc gctgacgttc gtcagaaagt tgacctgagt
     7621 accttcagcg atgaagaagt tatgcgtctg gctgaaaacc tgcgcaaagg tatgccaatc
     7681 gcaacgccgg tgttcgacgg tgcgaaagaa gcagaaatta aagagctgct gaaacttggc
     7741 gacctgccga cttccggtca gatccgcctg tacgatggtc gcactggtga acagttcgag
     7801 cgtccggtaa ccgttggtta catgtacatg ctgaaactga accacctggt cgacgacaag
     7861 atgcacgcgc gttccaccgg ttcttacagc ctggttactc agcagccgct gggtggtaag
     7921 gcacagttcg gtggtcagcg tttcggggag atggaagtgt gggcgctgga agcatacggc
     7981 gcagcataca ccctgcagga aatgctcact gttaagtctg atgacgtgaa cggtcgtacc
     8041 aagatgtata aaaacattgt ggacggcaac catcagatgg agccgggcat gccagaatcc
     8101 ttcaacgtat tgttgaaaga gattcgttcg ctgggtatca acatcgaact ggaagacgag
     8161 taattctcgc tcaaacaggt cactgctgtc ggggtaaaac ccggcagcgg attgtgctaa
     8221 ctccgacggg agcaaatccg tgaaagattt attaaagttt ctgaaagcgc agactaaaac
     8281 cgaagagttt gatgcgatca aaattgctct ggcttcgcca gacatgatcc gttcatggtc
     8341 tttcggtgaa gttaaaaagc cggaaaccat caactaccgt acgttcaaac cagaacgtga
     8401 cggccttttc tgcgcccgta tctttgggcc ggtaaaagat tacgagtgcc tgtgcggtaa
     8461 gtacaagcgc ctgaaacacc gtggcgtcat ctgtgagaag tgcggcgttg aagtgaccca
     8521 gactaaagta cgccgtgagc gtatgggcca catcgaactg gcttccccga ctgcgcacat
     8581 ctggttcctg aaatcgctgc cgtcccgtat cggtctgctg ctcgatatgc cgctgcgcga
     8641 tatcgaacgc gtactgtact ttgaatccta tgtggttatc gaaggcggta tgaccaacct
     8701 ggaacgtcag cagatcctga ctgaagagca gtatctggac gcgctggaag agttcggtga
     8761 cgaattcgac gcgaagatgg gtgcggaagc aatccaggct ctgctgaaga gcatggatct
     8821 ggagcaagag tgcgaacagc tgcgtgaaga gctgaacgaa accaactccg aaaccaagcg
     8881 taaaaagctg accaagcgta tcaaactgct ggaagcgttc gttcagtctg gtaacaaacc
     8941 agagtggatg atcctgaccg ttctgccggt actgccgcca gatctgcgtc cgctggttcc
     9001 gctggatggt ggtcgtttcg cgacttctga cctgaacgat ctgtatcgtc gcgtcattaa
     9061 ccgtaacaac cgtctgaaac gtctgctgga tctggctgcg ccggacatca tcgtacgtaa
     9121 cgaaaaacgt atgctgcagg aagcggtaga cgccctgctg gataacggtc gtcgcggtcg
     9181 tgcgatcacc ggttctaaca agcgtcctct gaaatctttg gccgacatga tcaaaggtaa
     9241 acagggtcgt ttccgtcaga acctgctcgg taagcgtgtt gactactccg gtcgttctgt
     9301 aatcaccgta ggtccatacc tgcgtctgca tcagtgcggt ctgccgaaga aaatggcgct
     9361 ggagctgttc aaaccgttca tctacggcaa gctggaactg cgtggtcttg ctaccaccat
     9421 taaagctgcg aagaaaatgg ttgagcgcga agaagctgtc gtttgggata tcctggacga
     9481 agttatccgc gaacacccgg tactgctgaa ccgtgcaccg actctgcacc gtctgggtat
     9541 ccaggcattt gaaccggtac tgatcgaagg taaagctatc cagctgcacc cgctggtttg
     9601 tgcggcatat aacgccgact tcgatggtga ccagatggct gttcacgtac cgctgacgct
     9661 ggaagcccag ctggaagcgc gtgcgctgat gatgtctacc aacaacatcc tgtccccggc
     9721 gaacggcgaa ccaatcatcg ttccgtctca ggacgttgta ctgggtctgt actacatgac
     9781 ccgtgactgt gttaacgcca aaggcgaagg catggtgctg actggcccga aagaagcaga
     9841 acgtctgtat cgctctggtc tggcttctct gcatgcgcgc gttaaagtgc gtatcaccga
     9901 gtatgaaaaa gatgctaacg gtgaattagt agcgaaaacc agcctgaaag acacgactgt
     9961 tggccgtgcc attctgtgga tgattgtacc gaaaggtctg ccttactcca tcgtcaacca
    10021 ggcgctgggt aaaaaagcaa tctccaaaat gctgaacacc tgctaccgca ttctcggtct
    10081 gaaaccgacc gttatttttg cggaccagat catgtacacc ggcttcgcct atgccgcgcg
    10141 ttctggtgca tctgttggca tcgatgacat ggtcatcccg gagaagaaac acgaaatcat
    10201 ctccgaggca gaagcagaag ttgctgaaat tcaggagcag ttccagtctg gtctggtaac
    10261 tgcgggcgaa cgctacaaca aagttatcga tatctgggct gcggcgaacg atcgtgtatc
    10321 caaagcgatg atggataacc tgcaaactga aaccgttatt aaccgtgacg gtcaggaaga
    10381 gaagcaggtt tccttcaaca gcatctacat gatggccgac tccggtgcgc gtggttctgc
    10441 ggcacagatt cgtcagcttg ctggtatgcg tggtctgatg gcaaagccgg atggctccat
    10501 catcgaaacg ccaatcaccg cgaacttccg tgaaggtctg aacgtactcc agtacttcat
    10561 ctccacccac ggtgctcgta aaggtctggc ggataccgca ctgaaaactg cgaactccgg
    10621 ttacctgact cgtcgtctgg ttgacgtggc gcaggacctg gtggttaccg aagacgattg
    10681 tggtacccat gaaggtatca tgatgactcc ggttatcgag gggggtgacg ttaaagagcc
    10741 gctgcgcgat cgcgtactgg gtcgtgtaac tgctgaagac gttctgaagc cgggtactgc
    10801 tgatattctc gttccgcgca acacgctgct gcacgaacag tggtgtgacc tgctggaaga
    10861 gaactctgtc gacgcggtaa aagtacgttc tgttgtatct tgtgacaccg actttggtgt
    10921 atgtgcgcac tgctacggtc gtgacctggc gcgtggccac atcatcaaca aaggtgaagc
    10981 aatcggtgtt atcgcggcac agtccatcgg tgaaccgggt acacagctga ccatgcgtac
    11041 gttccacatc ggtggtgcgg catctcgtgc ggctgctgaa tccagcatcc aggtgaaaaa
    11101 caaaggtagc atcaagctca gcaacgtgaa gtcggttgtg aactccagcg gtaaactggt
    11161 tatcacttcc cgtaacaccg aactgaaact gatcgacgaa ttcggtcgta ccaaagaaag
    11221 ctacaaagtg ccttacggtg cggtactggc gaaaggcgat ggcgaacagg ttgctggcgg
    11281 cgaaaccgtt gcaaactggg acccgcacac catgccggtt atcaccgaag taagcggttt
    11341 tgtacgcttt actgacatga tcgacggcca gaccattact cgtcagaccg acgaactgac
    11401 cggtctgtct tcgctggtgg ttctggattc cgcagaacgt accgcaggtg gtaaagatct
    11461 gcgtccggca ctgaaaatcg ttgatgctca gggtaacgac gttctgatcc caggtaccga
    11521 tatgcctgcg cagtacttcc tgccgggtaa agcgattgtt cagctggaag atggcgtaca
    11581 gatcagctct ggtgacacct tggcgcgtat tccgcaggaa tccggcggta ccaaggacat
    11641 caccggtggt ctgccgcgcg ttgcggacct gttcgaagct cgtcgtccga aagagccggc
    11701 aatcctggct gaaatcagcg gtatcgtttc cttcggtaaa gaaaccaaag gtaaacgtcg
    11761 tctggttatc accccggtag acggtagcga tccgtacgaa gagatgattc cgaaatggcg
    11821 tcagctcaac gtgttcgaag gtgaacgtgt agaacgtggt gacgtaattt ccgacggtcc
    11881 ggaagcgccg cacgacattc tgcgtctgcg tggtgttcat gctgtgactc gttacatcgt
    11941 taacgaagta caggacgtat accgtctgca gggcgtaaag attaacgata aacacatcga
    12001 agttatcgtt cgtcagatgc tgcgtaaagc taccatcgtt aacgcgggca gctccgactt
    12061 cctggaaggc gaacaggttg aatactctcg cgtcaagatc gcaaaccgcg aactggaagc
    12121 gaacggcaaa gtgggtgcaa cttactcccg cgatctgctg ggtatcacca aagcgtctct
    12181 ggcaaccgag tccttcatct ccgcggcatc gttccaggag accactcgcg tgctgaccga
    12241 agcagccgtt gcgggcaaac gcgacgaact gcgcggcctg aaagagaacg ttatcgtggg
    12301 tcgtctgatc ccggcaggta ccggttacgc gtaccaccag gatcgtatgc gtcgccgtgc
    12361 tgcgggtgaa gctccggctg caccgcaggt gactgcagaa gacgcatctg ccagcctggc
    12421 agaactgctg aacgcaggtc tgggcggttc tgataacgag taatcgttaa tccgcaaata
    12481 acgtaaaaac ccgcttcggc gggttttttt atgggtgtta ttcagagggc aaattcttca
    12541 cggaaaatat taagaaaggc agggcgaaac gaggaagaag cttttcgccc tggacaacat
    12601 tcctgctgac ggcactacca taaaaaaaca caacgctttc aacaaccttg cgtttttcat
    12661 cttccgtagc ggctcggaat aaatttacgt tcttacattc cgtctcatag tctttgcgag
    12721 gcgcgtccca gatagctgtc ccagtctttc cataccggct gcaaaccctg agcggttaac
    12781 acggcagcaa ccgcttccgg tctgcgatcg tcgtgcggtg agaactgttc caactcgggg
    12841 tgattatcgg catagccacc tggctgcgtt ttcgagaagg cgctgacgtt attgatcgcc
    12901 agcggaataa cgcgatcgcg aaaccacggt gattcccgcg tggagagtga cagttcaatc
    12961 tccggtgcaa gcagtcggaa ggcgcagatg gtttgcacta actggcgttc atccataatc
    13021 gacgcaggct caatgccgcc agtacacggg cgcaggcgcg gaaaggagac agagtaacgg
    13081 ctttgccagt aatgctgttg cagccatagc aaatgttcgg caaccatata gcagtcaacg
    13141 cgccagttgt cggaaaggcc aattagcgcg ccgaggccta tcttatcaat ccccgcacgc
    13201 cccagccgat ccggcgtttc cagccgccag aagaagtcct gttttttgcc tttcaaatga
    13261 tggcgggtat aagtcgcctc gtgatatgtc tcctgataaa ccatcacccc atccagacca
    13321 agttgcttta actcggcgta ttccgtctcc gccagcggtt gcacttccat ttgtagtgaa
    13381 gagaactgtt cacgcagggc ggggagatga cgacgaaagt aatccatccc cactttcgcc
    13441 tgatgttcac cagtgactaa tagcagatgt tcaaagccca tctcccgtat agcggcgctt
    13501 tccctggcaa tatccgcttc atccagcgtt ttgcgcttga tgcgattact catggaaaat
    13561 ccgcagtacg tgcagtcgtt agcgcaaaga ttggaaagat aaagcgggac gtagaaacta
    13621 actgtgttgc caaatcgctg acgggtcaga cgctgcgcct gttgggccag ttgttccaga
    13681 tagccactgg cggcaggcga taacagcgcc atcatgtcgt cgcgggtgag ttgcgaggca
    13741 tttagcgccc gctctacgtc agcagccgtt ttgccgttga tacgcaggcg gatgtcgtcc
    13801 cagtccagtt gtcgccagcg attgctgaag gttttcatgc cgatgcctcc agaaatccgg
    13861 tcagcgggct ggtggcatga gcaaaataac tgcggctgcc cggtccggac tgacgtgcca
    13921 gcaggcctac ttctaccgcc agacgaaatg ccttcgccat gttgacggga tcgtccgcga
    13981 cggcaatcgc cgtattcact aacaccgcgt cggcccccat ttccagcgcc tgcgtggcat
    14041 ggctgggaac gccgatgcca gcatcgacaa ccaccggaac tgtggcctgc tggataataa
    14101 tctccagcat ggcacgggtt tccagtccct gattcgagcc aatcggcgcg ccgagcggca
    14161 tcaccgctgc acagccgact tcttccagac gtttacacaa taccggatcg gccccgcagt
    14221 aaggcagcac gacaaatccc tgttgtacca gcgtttcggc ggctttcagg gtttcgatgg
    14281 gatcgggcaa cagccagcgg gcgtcagggt gaatctccaa ttttagccag tttgtgccta
    14341 acgcttcacg tgccagatgg gcggcgaaaa tggcttcttc cgctgttttc gccccggaag
    14401 tattcggcag cagggccaca cccgccgcga taagcggttc gaggatagcg tcgttgtgct
    14461 ggcgcaagtc gacacgtttc atcgccagtg tcaccagctg gctgccggaa gcgcggatcg
    14521 cctccaccat cagttgtgaa gaagcgaatt tccctgtgcc ggtaaacaga tgtgaatcaa
    14581 acgttttgtc cgcaatacgt aacatctcaa ccccctgcaa taacctgaaa aagcaggatc
    14641 tggtcgccat cctgcacgat atgttgcgtc cactgctcac gcgggacgat ttgctgatta
    14701 atcgccagag ccgcgcccgc ttgtcgttgg tcgagttgct ccagtagttc gtgaacagtt
    14761 tgcccggcgg cgcactgcat cggttgatcg ttaaacagga tctgcattgc ttcctccgca
    14821 taccgggcaa ccactggcgc ggcgcaacgc caggctgcgc cactggctcg acttaccgtc
    14881 gaacagtcgg agttctcccg caggcgtctc tataccgctt aataacttaa tggcttccag
    14941 tgcctgcaaa gtgcccatca ccccgaccac cgggccaacc acgcccgccg tgcggcagtt
    15001 gcgttctggc tgctggttat ctggccacag gcagcggtaa cacccctgct cccagggcgg
    15061 tgtcagtacc atcaactgac cgccaaatcc gaccgcgctg gcggtgataa gcggcgtgtt
    15121 gagtgccacg caggcggcat taatctcctg gcgagtcgcc atattgtcgg tacagtcgag
    15181 caccacatcg gcttgtgcaa ccgcatcttt caacgcctca cccgttaacc gttgttgtaa
    15241 tgctgtcagt tgaatgtcgg gattcaactg tgtcagtcgc tgttggctga cctgcgattt
    15301 cgggctgttg atatcttcag tggtaaagag gatttgtcgt tgcagattgc ttaaatgcac
    15361 atcgtcgtca tctgccagta ccagcgtccc gacgccagcc cctgccaggt atagcgcagc
    15421 aggtgtaccc agcccgccaa gaccaataat cagcacctgg ctgtcgagca gtttttgctg
    15481 cccgtcaaga gcgatatcgt cgagcagaat ttggcggcta taacgcataa agtcacggtc
    15541 attcatcgcc aactcctgca atttccagca actgtgccgt tgccaaacgc cagtctgcgg
    15601 cttgagtaat ggcgctgacg acggcgatac tgccgacacc cgttgctatc accgcaggcg
    15661 cgcgtgccag actgataccg ccaatcgcca cggtgggata atccgccagg cgctcaacat
    15721 gccgtgccaa ctgttccagc ccctgcggtg cagaaggcat ctgtttggtt tgcgtcggga
    15781 acacatgtcc cagcgcgata taagaggggc gtgctgccag cgcgacgtcg atttccatat
    15841 cgtcatgtgt cgaaacgccc agccgcaggc ctgccgcgcg gatggcattg agatcggtgg
    15901 cttgcaaatc ttcctgcccc aaatggacgc catacgcctg atgcttgatc gcgagccgcc
    15961 agtaatcgtt gataaacaat cgcgcgttat agcggcggcc cagcgcaatt gccgccacga
    16021 catcggcttc cacctcttca tcgcgccgat ctttgatgcg tagctggaga gtacgtacgc
    16081 ctgcatccaa cagacgttcg atccactgta cgctgtccac caccgggtac agtcctaaac
    16141 gaaaaggtac aggaggaaaa tcaggctgat acatcacgct tcctccttat gcaggtagat
    16201 ttctccgcct ctggcacgga agttctccga catatccgcc attcccactt caatggtttg
    16261 cgcggcggcg taatcgcgta cttcctggct gattttcatc gagcagaatt ttggcccaca
    16321 catggagcaa aaatgggcga ctttgccgga ctcttggggc agggtttcat cgtgataagc
    16381 gcgggcggta aacgggtcga gggccagatt aaactggtct tcccagcgaa attcgaagcg
    16441 ggctttcgac atggcgttat cgcgaatttg cgcgcccgga tgcccttttg ccaggtcagc
    16501 agcgtgggcg gcaatcttat aagtgataag cccctgctta acatcttctt tattgggcag
    16561 acccagatgc tcttttggcg ttacgtaaca gagcatcgcg cagccaaacc agccaatcat
    16621 cgccgcacca atccccgacg tgaagtggtc atagcccggt gcaatatcgg tagttagcgg
    16681 ccccagagtg taaaacggcg cttcgtggca gtgctctaac tcctcggtca tattgcggcg
    16741 gatcatctgc atcggcacat ggcctgggcc ttcaatcatc acctgtacgt catattccca
    16801 ggcgattttg gtcagttcgc ccagcgtatg caattcggca aactgcgctt catcgttggc
    16861 gtcctgaata gaaccggggc gcagaccgtc gcccagcgac agcgaaacgt cataagcggc
    16921 acagatttca caaatttcgc ggaagtgttg atagaggaaa ttttcctgat gatgggagag
    16981 gcaccatttc gccataatcg aaccgccgcg agagacgata ccggtcaggc gtttcgcggt
    17041 catcggcaca tagcgcagca gtacgcccgc atggatagtg aagtaatcca caccttgctc
    17101 ggcctgttcc agcagcgtgt cgcggaacgc ttcccaggta agatcttcgg cgatcccgtt
    17161 aaccttctcc agcgcctggt agatcggcac tgtaccgatc ggcaccggac tgttacgcaa
    17221 aatccactcg cgggtttcgt gaatatagcg accggtggag agatccatca ccgtatccgc
    17281 tccccagcgc gtggaccata ccagcttttc cacttcttct tcgatggaag aggtgaccgc
    17341 cgagttgccg atattggcgt taacttttac caggaagtta cggccaataa tcatcggctc
    17401 cgattccgga tgattaatgt tggccgggat aatcgcacgt ccggcagcaa cttcatcacg
    17461 gacaaattcc gcagtgatat tttccggcag atgtgcgcca aagctcattc ccggatgctg
    17521 gtggcgtaaa acctcgctac ggatgcgctc gcggcccata ttctcgcgga tggcgatgaa
    17581 ttccatttcc ggcgtgatga tgccctggcg ggcgtagtgc agttgggtga cacggcgtcc
    17641 tgttttggcg cgttttggcg ttaataagcc gctaaaacgt agctcgtcga gaccatcatc
    17701 tgccagccgc gttctggtgt aatcggaact gcgcacggta agttcttcgg tatcggcgcg
    17761 cgcatcgatc cacggctggc gtagttttgc cagcccttgc tgcacgttaa tggcgatctg
    17821 cggatcacca tacgggccgg aggtgtcgta gaccggaatc gcttcgtttt cttcgtactg
    17881 cggctgttct ttgctaccgc caattagcgt cgggctaagc tggatctcac gcatcggcac
    17941 gcgcacgccg gggtgtgtgc cagtgatata aatgcgtttt gagttgggaa aggcggtgcc
    18001 ttccagggtg tcgataaaat gttgggcccg ggcgcgttgt tcgcggcggg tcagttttgt
    18061 tgcagacata gctcattcca aaaagttaag gacgtggctt gtcagacgac ggatgaagca
    18121 agagacgatc gccgcaaagg cgatgcgata gcagattaac tcttgttccc ttcgcaggta
    18181 ttagcctgat caggttccgc ggatcccgaa taaacggtct cagccagtta aggcactccg
    18241 acaagaaatt agcccccaca aacggggcgt tgaatgtaaa ttacgcgtta acagcgcaga
    18301 actcaagcag gatgtttgac gcgggcggcg tcaagcacca gcagaatcag cttatcttcc
    18361 agcacaaagc gcgcttccag cgcctcgccg atgtcagata aaacctgttg aaactcaagg
    18421 taattatcat gatcgatggc ggtttccagg ctggaatcgt agtaatccat aatctgttgg
    18481 gtattggctt cgagttgcgg ccaaatcttc gcggcgcgcg cgagttgccc gttgccttcc
    18541 agcttatgaa gaatgcgctc ataaatactg aaatgtccgg cagacaagta atcgaccagg
    18601 ctctgacaaa aatcatcaag ggctttttcg tttagcctca tgtacgattc tttgccaggc
    18661 ttaatgccaa ccagattgta gtaagccacg agcagatgct tacgtacatg tagccagcga
    18721 tcaaccagtt tgttacttcc tctgacgcgc tccgtcaggt tatcgagctg gttaagcatg
    18781 attgactccg caagtttgta ttcaaaaact gctcagtgag aaatgtaaaa accatgttaa
    18841 acatgccagt gatgcaaagg tagtgcaaga gctatggatc gtataattga aaaattagat
    18901 cacggctggt gggtcgtcag ccatgaacaa aaattatggt tgccgaaggg agaattgcca
    18961 tatggcgaag cggcaaattt cgatcttgtg ggtcagcgcg cactacaaat cggcgaatgg
    19021 cagggggaac ctgtttggtt aatacaacag cagcggcgtc acgatatggg gtcggtacgt
    19081 caggtcattg atctcgatgt tgggctgttt caactggccg gacgtggcgt gcaactggcg
    19141 gagttttacc gatcgcataa atactgtggt tactgcgggc atgaaatgta tccgagcaaa
    19201 accgaatggg cgatgctgtg tagccattgc cgtgagcgtt actacccgca aatcgccccc
    19261 tgcattattg ttgccatccg tcgcgatgat tcgctcctcc tcgcccagca tacccgccat
    19321 cgtaacggtg tccatacagt acttgccgga ttcgtcgaag tgggcgaaac cctcgagcag
    19381 gcagtcgcgc gggaagtgat ggaagagagc ggaattaaag ttaaaaactt gcgttacgtg
    19441 acttctcagc cgtggccgtt tcctcagtct ttaatgaccg cgtttatggc ggaatatgac
    19501 agcggcgaca tcgtgatcga cccgaaagaa ttgctcgagg cgaactggta tcgctatgac
    19561 gatttgccgt tactcccgcc gcccggcacc gtagcgcgcc gtctgataga agatactgtg
    19621 gcgatgtgtc gggcagagta tgagtgatga tacactgacc gcctgacgca ctaaggaaca
    19681 gccaaaatga ccgaacttaa aaacgatcgt tatctgcggg cgctgctgcg ccagcccgtt
    19741 gatgtcactc cagtatggat gatgcgccag gcgggtcgct atctaccgga atataaagcc
    19801 acgcgcgccc aggcgggcga ttttatgtcg ctgtgcaaaa acgccgagct ggcgtgcgaa
    19861 gtgactttgc aaccgctgcg tcgctacccg ctggatgcgg cgatcctctt ttccgatatc
    19921 ctcaccgtgc cggacgcgat ggggttaggg ctctattttg aagccggaga aggtccgcgt
    19981 tttacctcgc cagtcacctg caaagccgac gtcgataaac tgccaattcc ggacccggaa
    20041 gatgagctgg gttacgtgat gaacgcggtg cgtaccattc gtcacgaact gaaaggcgaa
    20101 gtgccgctga ttggtttttc cggcagcccg tggacgctgg caacctacat ggtggaaggc
    20161 ggcagcagca aagccttcac cgtgatcaaa aaaatgatgt atgccgatcc gcaggcgctg
    20221 cacgctctgc tcgataaact ggcgaaaagc gtcactttgt atctgaatgc gcagattaaa
    20281 gccggtgctc aggcagtgat gattttcgac acctggggcg gcgtgcttac cgggcgcgat
    20341 tatcaacagt tctcgctcta ttacatgcat aaaattgttg atggtttact gcgtgaaaac
    20401 gacggtcgcc gcgtgccagt cacgctgttt accaaaggcg gcggacagtg gctggaagcc
    20461 atggctgaaa ccggttgcga tgcgctgggc ctcgactgga caacggacat cgccgatgcg
    20521 cgccgccgtg tgggcaataa agtcgcgttg cagggtaata tggatccgtc gatgctgtac
    20581 gcgccgcctg cccgcattga agaagaagta gcgactatac ttgcaggttt cggtcacggc
    20641 gaaggtcatg tctttaacct tggtcacggc attcatcagg atgtgccgcc agaacatgct
    20701 ggcgtgttcg tggaggcagt gcatcgactg tctgaacagt atcaccgcta agagcctgat
    20761 ccatcaggct attttatttg ccattttgag gccgggcagt gctcaaaatc ctcacgtact
    20821 acgtgtacgc tccggttttt ccgcgctgtc cggcctcaaa ctgccttcaa caattacgcc
    20881 tgttgaacca ggctctaagg agcgattatg gatctcgcgt cattacgcgc tcaacaaatc
    20941 gaactggctt cttctgtgat ctgcgaggat cgactcgata aagatccacc ggatctgatc
    21001 gccggagccg atgtcgggtt tgagcagggc ggagaagtga cgcgagcggc gatggtgctg
    21061 ctgaaatatc cctcgcttga gctggtggag tataaagttg cccgcatcgc caccaccatg
    21121 ccttacattc caggttttct ttccttccgc gaatatcctg cgctgctggc agcgtgggag
    21181 atgctgtcgc aaaagccgga tttagtgttt gtcgatggtc atgggatctc gcatcctcgc
    21241 cgtcttggcg tcgccagcca ttttggctta ttggtggatg tgccgaccat tggcgtggcg
    21301 aaaaaacggc tctgcggtaa attcgaaccg ctctccagca aaccgggcgc gctggcccca
    21361 ctgatggata aaggcgagca gctggcctgg gtctggcgca gcaaagcgcg ctgtaacccg
    21421 ttgtttatcg ctaccggcca tcgggtcagc gtggacagcg cgctggcgtg ggtacaacgc
    21481 tgcatgaaag gctatcgtct gccggagcca acgcgctggg cggacgcggt ggcctcggaa
    21541 cgtccggcgt tcgtgcgcta tacagcaaat cagccctaat tcaggtaaac tgcggataat
    21601 ttccgtattt gagaactcaa catgttacaa aacccaattc atctgcgtct ggagcgcctg
    21661 gaaagctggc agcatgtcac tttcatggct tgcttatgcg aacgcatgta ccccaattac
    21721 gccatgttct gccagcaaac cggttttggt gatgggcaaa tttaccgtcg tattctcgat
    21781 ctcatctggg aaacgctgac cgttaaagac gcaaaagtaa atttcgacag ccaactggag
    21841 aaatttgaag aagcgattcc ttcagccgac gatttcgatc tgtacggcgt ttatccggca
    21901 atcgatgcct gcgtggcgtt aagtgaactg gtccattcgc gtttgagtgg tgaaacgctc
    21961 gaacacgcgg tggaagtgag taagacctcc atcacgaccg ttgcgatgct ggaaatgact
    22021 caggctggtc gcgaaatggg cgatgaagag cttaaagaaa acccagctgt agagcaagaa
    22081 tgggacattc agtgggaaat attccgactt ttagctgagt gcgaagaacg cgatatcgag
    22141 ctgataaaag gccttagggc agacctgcgt gagtcgggtg agagcaatat tggtataatt
    22201 tttcagcaat aagaccagaa aacgtgattt aacgcctgat ttgtcgtacc tggagtcttc
    22261 cctttcgccc cccgtctggt ctacatttgg ggggcgaaaa aaagtggcta tcggtgcgtg
    22321 tatgcaggag agtgctattc tggcatttcc gtcgcactcg atgcttagca agcgataaac
    22381 acattgtaag gataacttat gaacaagact caactgattg atgtaattgc agagaaagca
    22441 gaactgtcca aaacccaggc taaagctgct ctggagtcca ctctggctgc aattactgag
    22501 tctctgaaag aaggcgatgc tgtacaactg gttggtttcg gtaccttcaa agtgaaccat
    22561 cgcgctgagc gtactggccg caacccgcag accggtaaag aaatcaaaat tgccgcagct
    22621 aacgtaccgg catttgtttc tggcaaggca ctgaaagacg cagttaagta agattgcgtg
    22681 gcagtgaaca gttttaacga aggggtggtt tcaccccttt tgtctttctg gcgtcgatca
    22741 ttgatgctgg caggtgcgct gtttctcact gcctgtagcc acaactcttc acttcctccc
    22801 tttaccgcca gtggatttgc tgaagaccag ggcgcggtac gcatctggcg aaaagacagc
    22861 ggcgataatg tgcatctgct tgccgtgttt agcccgtggc gcagtggcga taccacgacg
    22921 cgagagtatc gctggcaggg cgataacctc acgctcatca atatcaatgt ttacagcaaa
    22981 ccgccggtga acattcgcgc gcgttttgac gatcgcggcg atctgagctt tatgcaacgt
    23041 gaatccgatg gggaaaagca gcagctttct aacgaccaaa tcgatttata ccgttatcgt
    23101 gctgatcaga tccgccagat tagcgatgcc ttacgtcagg ggagagtcgt gctgcgccag
    23161 gggcgctggc atgcgatgga acagaccgtg accacctgcg aagggcaaac cattaaacct
    23221 gatttagatt cgcaggcgat agcgcatatc gagcgccgcc agagccgctc ttctgttgat
    23281 gtcagcgtgg catggctgga agcgcccgaa ggttcgcaat tactgttagt ggcaaactct
    23341 gatttctgtc gctggcaacc caacgagaaa acgttctgat ttaccagtgg cccataccca
    23401 tatgaccgcc accaccgcag ccgccgtagc ccatgcccat tccggctccg cgcggaatac
    23461 ccgcttcagc catcgcgata tctcgtttca cccgtaactc atctaacgac tgacgcaaat
    23521 tctccatctc tttggcgacc gcgttaattt tgctgctatc cggtgggttc gcggctaaca
    23581 gggcattgta ttcataacgc ttcgtcacca gttgctgttg cagtgcgctg ctttgagcgt
    23641 aaaagtcatt atggattttc tgccacgctg tctgttgttc gctggtcaaa ggcgcggcat
    23701 tttgctgcca cataccgtgt ccgccgtgag caaatgcaga tgtcgatccc atcgccattg
    23761 ctgaaagcgc catcattacc agggcaattt tcgtgttccg tttcatggtt aatcctccag
    23821 tggttgtctt acttcgggta ttgcatcttc cgtgccaacg atgaaacgct gatatgacgg
    23881 gtaatctggc atgataaacg agtaaaaatg actcgcctgc tgcgggtagc gagtcatttt
    23941 tactcattga aacttaagcc tttgtgttac agcgcagggt aagcgctgat aaaagatggc
    24001 atgatttctg ctgtcagaaa gggatgagca ggcaaagaag aagatgcgtt ttatgcaacg
    24061 ttctaaagac tccttagcta aatggttaag cgcgatcctc cccgtggtca ttgttgggct
    24121 ggtagggttg tttgcggtga cggtgattcg cgattatggg cgcgagactg ccgccgccag
    24181 acaaacgctg ctggaaaaag gcagtgtact tattcgcgct cttgaatccg gctcgcgcgt
    24241 cggcatgggg atgcgcatgc atcatgcgca gcagcaggcc ttactggaag aaatggccgg
    24301 gcagcctgga gtacgttggt ttgcggtcac ggatgaacaa ggaacaatcg tgatgcatag
    24361 caactccggc atggtgggaa aacagcttta ttcccctcag gaaatgcagc agttacatcc
    24421 gggagatgaa gaagcgtggc ggcggatcga tagcgcagac ggcgagcctg ttctggaaat
    24481 ttatcgccag tttcaaccga tgtttgctgc tggaatgtac cggatgcgcc atatgcagca
    24541 gtatgccgcg acaccacaag caattttcat cgctttcgac gccagtaata ttgtgagtgc
    24601 cgaagatcgt gagcagagaa acaccctgat tattctcttc gccctggcga cggtcttgct
    24661 ggcaagcgta ttgtcattct tctggtatcg ccgctatctg cgctcgcgcc agcttctaca
    24721 agatgaaatg aagcgcaaag agaagctggt ggcgctgggg catcttgcgg caggcgttgc
    24781 ccacgaaatc cgtaacccac tttcctcgat taaaggactg gcgaaatact ttgccgagcg
    24841 cgcgcctgca gggggagaag cgcatcaact ggcgcaggtg atggcgaaag aggccgaccg
    24901 tttaaaccgc gtggtaagcg agttgctgga actggttaag ccaacgcatc tggctttgca
    24961 ggcggtggat ctcaacacgc tgattaacca ctcattacag ctggtaagcc aggatgcaaa
    25021 cagccgggag atccagttac gctttaccgc caacgacaca ttaccggaaa ttcaggccga
    25081 tccggacagg ctgactcagg tcctgttgaa tctctatctc aatgctattc aggcgattgg
    25141 tcagcatggc gtgattagcg tgacggccag cgaaagcggc gcgggcgtga aaatcagcgt
    25201 taccgacagc ggtaagggaa ttgcggcaga tcagcttgaa gccatcttca ctccgtactt
    25261 caccaccaaa gccgaaggca ccggattggg gctggcggtc gtgcataata ttgttgaaca
    25321 acacggtggt acaattcagg tcgcaagcca ggagggaaaa ggctcaacgt tcaccctctg
    25381 gcttccggtc aatattacgc gtaaggaccc acaaggatga cgcacgataa tatcgatatt
    25441 ctggtggtgg atgatgacat tagccactgc actattttgc aggctttact gcgcggctgg
    25501 ggctataacg tcgcgctggc gaacagcggg cgacaggcgc tggagcaggt gcgggaacag
    25561 gtttttgatc ttgtgctttg cgatgtgcga atggcggaga tggacggcat cgccacgctg
    25621 aaagagatca aagcgttaaa cccggcaatt ccggtgctga ttatgactgc gtactccagc
    25681 gtcgagacgg cggtagaggc actgaaaact ggggcgcagg attatctcat caagccgctg
    25741 gatttcgata acctacaggc gacgctggaa aaggcgctcg cgcatacgca cagtattgat
    25801 gctgaaacac ctgcggtgac tgccagccag ttcggtatgg tcggtaaaag cccggcgatg
    25861 caacacctgc tcagtgaaat cgccctcgtc gcgccatcgg aagccacggt gctgatccac
    25921 ggcgattccg gcaccggtaa agagctggtc gccagggcga ttcacgccag tagcgcacgt
    25981 agcgaaaaac cgctggtaac gctcaactgt gcggcactca acgaatcctt gctggaatct
    26041 gaattgttcg gtcacgaaaa aggggcgttt actggagccg ataaacgccg ggaagggcgc
    26101 tttgttgagg cggacggcgg cacgctgttt ctcgatgaaa ttggcgatat ctcgccgatg
    26161 atgcaggtgc gtctgctgcg tgcgattcag gagcgcgaag ttcagcgtgt cggtagcaac
    26221 cagactatct cggttgatgt ccggctgatt gcggcgaccc atcgcgatct tgccgcagag
    26281 gtgaatgccg ggcgttttcg ccaggatctc tactatcgcc tgaatgtggt ggcgattgaa
    26341 gtaccatcgc tgcggcaacg gcgggaagat atccctctgc tggctggcca ttttcttcag
    26401 cgctttgccg agcgtaatcg caaggcggta aaaggcttta cgccacaggc gatggatttg
    26461 ctgatccact acgactggcc gggaaatatt cgtgaactgg aaaacgcggt ggaacgggcg
    26521 gtggtgctgc tgaccgggga atatatttcc gaacgcgagc tgccgctggc gattgccagt
    26581 acgccgatcc cgctggggca aagtcaggat attcagccgc tggtggaagt ggaaaaagag
    26641 gtgattctgg cggcgctgga gaaaacgggc ggcaacaaaa ccgaagccgc ccgtcagtta
    26701 gggatcacgc gcaaaacgct attggcaaaa ctgtcgcgtt agttctgctc gcgttcgatg
    26761 gcgcgccaac cgatatcttt ccggcagaag caatcatccc agtggatatc ggtcattaag
    26821 gcgtaggcgc gtttctgcgc ttctgccacg gtatgaccca gcgcggtgac gcacagtacg
    26881 cgcccgccgc tggttaccac ctgctcgtca tccgccagtt ttgtgcccgc gtggaacact
    26941 ttgccgtcag ccacttcttc cagcggcagg ccgtggatca catcgccggt gcggtaatcg
    27001 cccggatatc cacccgcagc catcaccacg ctgagagaag cacgttcatc ccactctgac
    27061 gttttctcgt ccagtttgcc ttcacaggcc gccaggcaga gctcaaccag atcggacttc
    27121 atgcgcagca taatcggctg ggtttccgga tcgccaaagc ggcagttaaa ttcaataacc
    27181 ttcggattgc cctgtttgtc gatcatcagg cccgcataga gaaaaccggt gtaggtgttg
    27241 ccttccgccg ccatgccttt cacggttggc cagatgatac gttccatggt gcgctgatga
    27301 acttcatcgg ttactaccgg cgcgggggag taagcgccca tcccgccggt gtttggtccg
    27361 ttatctttat cgcctacgcg tttgtgatcc tggctggtag ccatcggcag cacatgctcg
    27421 ccgtccacca tcacgataaa gctcgcttct tcgccatcga ggaactcttc gataacgatg
    27481 cgatgacccg cgtcgccaaa agcgttgccc gccagcatat cgtgaacagc cgcttccgct
    27541 tcttccagcg tcatcgccac gataacgcct ttcccggcag ccagaccgtc cgctttaatg
    27601 acgattggcg cgcctttctc acgcagatac accagcgcag gttctacctc ggtgaagttc
    27661 tggtattccg ccgtagggat cttatggcgg gccaggaaat ctttggtaaa cgcttttgag
    27721 ccttccagtt gggccgcacc tgcggttggg ccgaagattt tcagcccggc ggcgcggaag
    27781 gtatcgacca cgcctttcac cagcggcgct tccgggccga cgatggtcag atcaatcttt
    27841 tcgttttgtg cgaaatccaa cagcgccggg atatcggtca cgccgatagc aacgttttgc
    27901 agcgcgggtt ccagcgcagt gcctgcatta cccggagcag caaaaacagt ctcaaccagc
    27961 ggcgactggg ccgctttcca ggccagcgcg tgctcgcgcc cgccgttacc aatcactaat
    28021 actttcatct attgctccat taatggcgga agtggcgcat gtcggtgaag agcatcgcaa
    28081 taccgtgctc gtcggcggcg gcaatcactt cgtcatcacg gatggaaccg ccaggctgga
    28141 ttacgcaggt cacgcccgca gcggcggcgg catcaatacc gtcgcggaac gggaagaacg
    28201 cgtcagaagc catcgaggaa cctttcactt ccaggccttc atcggcagct ttaataccgg
    28261 cgattttcgc ggagtacacg cggctcatct ggcccgcgcc aataccgatg gtcatattgt
    28321 ttttggcata gacgatagca ttggatttca cgaatttcgc cactttccag cagaacagcg
    28381 catcacgcag ttcctgttcg gtcggctgac gttgggtgac gacgcgcagt tcttccgcac
    28441 cgaccatccc caggtcacga tcctgaacca gcagaccgcc gttcacgcgt ttgaaatcaa
    28501 gacccggaac acgctcgccc cactgaccgc aggtcagaac gcgtacgttc tgcttggcgg
    28561 cggtgatttt cagggcttct tcgctggcgg aaggcgcaat aatcacttcg acaaactgac
    28621 gagaaatgat ggcctgtgcg gtttccgcat ccagctcgcg gttaaaggca atgatgccgc
    28681 cgaatgcgga ggttgggtcg gttttgtacg cgcgatcgta agcatcaaga atggaattgc
    28741 cgatagctac accgcaaggg ttggcgtgct tcacaatcac acatgccggc tcggcgaact
    28801 ctttcacgca ctccagcgcc gcatcggtat cggcgatgtt gttataagag agggctttac
    28861 cctgaacctg ggttgcggta gcaacggagg cttctttcac attctcttct atatagaagg
    28921 cagcctgctg gtggctgttc tcgccgtaac gcatatcctg cttcttaatg aagttcaggt
    28981 tcagcgtgcg tgggaagcga ccggcggctt ctttgctttc accgtggtac gccggaacca
    29041 tgctgccgaa gtagttggca atcatgctgt cgtaggcggc agtgtgttcg aaggctttga
    29101 tggcgaggtc gaaacgggtt gcaagcgtca gcgatccttc gttgtcatcc atctctttaa
    29161 taatggcgtc gtagtcgctg ctcttcacca cgattgcgac atctttatgg ttcttagcgg
    29221 cggagcgcac catggtcggg ccgccgatat cgatgttctc aaccgcatct tccagcgagc
    29281 agccttcacg agcaacagtc tgggcgaacg gatacaggtt aacaaccacc atatcgatag
    29341 gctggatctg atgttcttcc ataatggcat cgtcctggcc gcgacggccc agaatgccac
    29401 catgtacttt cggatgcagg gtcttcacgc gtccatccat catctccggg aaaccggtgt
    29461 aatcggaaac ttcggttacc ggcagacctt tttctgctaa cagacgggca gtgccccctg
    29521 tagacagcag ctccacaccg cgtgcggaaa gtgcctgggc gaattcgacg ataccggctt
    29581 tgtcagaaac actgagcaga gcgcggcgga ctggacgacg ttgttgcatg gtaaatcccc
    29641 tgggtttgac tattacagag agcgttagct gaatttttcg cgaaaaactc agctaacgcc
    29701 cctaacgggg catccttatt tttcgcccgc attgtaacga aaacgtttgc gcaacgctcg
    29761 cgaatttttc tctttcaatg gtgatcacaa ttttgacagt ggttaccgtg ggaaaaatac
    29821 agaaattaca ttgatgattg tggataactc tgtgcgtaaa aaggtataaa gcggggtttt
    29881 gctggggaat gcagcagtca gtcatttttc tgcaattttt ctattgcggc ctgcggagaa
    29941 ctccctataa tgcgccttca tcgacacggc ggatgtgaat cacttcacac aaacagccgg
    30001 ttcggttgaa ga
//
LOCUS       DAJTRC010000045        29658 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.045, whole genome shotgun sequence.
ACCESSION   DAJTRC010000045 DAJTRC010000000
VERSION     DAJTRC010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29658)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29658)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29658
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.045"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(1..441)
                     /locus_tag="OO357_002128"
                     /pseudo
     CDS             complement(1..441)
                     /locus_tag="OO357_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310631.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-6-phosphate synthase"
     gene            complement(416..1216)
                     /gene="otsB"
                     /locus_tag="OO357_002129"
     CDS             complement(416..1216)
                     /gene="otsB"
                     /locus_tag="OO357_002129"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HCR5887075.1"
                     /translation="MTEPLTETPELSAKYAWFFDLDGTLAEIKPHPDQVVVPDNILQG
                     LQLLATASDGALALISGRSMVELDALAKPYRFPLAGVHGAERRDINGKTHIVHLPDAI
                     ARDISVQLHTVIAQYPGAELEAKGMAFALHYRQAPQHEDALMTLAQRITQIWPQMALQ
                     QGKCVVEIKPRGTSKGEAIAAFMQEAPFIGRTPVFLGDDLTDESGFAVVNRLGGMSVK
                     IGTGATQASWRLAGVPDVWSWLEMITAALQQKRENNRSDDYESFSRSI"
     gene            complement(1383..2369)
                     /gene="araH"
                     /locus_tag="OO357_002130"
     CDS             complement(1383..2369)
                     /gene="araH"
                     /locus_tag="OO357_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026162.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter permease AraH"
                     /protein_id="HCR5887076.1"
                     /translation="MSSVSTSGSGAPKSSFSFGRIWDQYGMLVVFAVLFIACAIFVPN
                     FATFINMKGLGLAISMSGMVACGMLFCLASGDFDLSVASVIACAGVTTAVVINLTESL
                     WIGVAAGLLLGVLCGLVNGFVIAKLKINALITTLATMQIVRGLAYIISDGKAVGIEDE
                     SFFALGYANWFGLPAPIWLTVACLIIFGLLLNKTTFGRNTLAIGGNEEAARLAGVPVV
                     RTKIIIFVLSGLVSAIAGIILASRMTSGQPMTSIGYELIVISACVLGGVSLKGGIGKI
                     SYVVAGILILGTVENAMNLLNISPFAQYVVRGLILLAAVIFDRYKQKAKRTV"
     gene            complement(2384..3898)
                     /gene="araG"
                     /locus_tag="OO357_002131"
     CDS             complement(2384..3898)
                     /gene="araG"
                     /locus_tag="OO357_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter ATP binding protein
                     AraG"
                     /protein_id="HCR5887077.1"
                     /translation="MQQSTPYLSFRGIGKTFPGVKALTDISFDCYAGQVHALMGENGA
                     GKSTLLKILSGNYAPTTGSVVINGQEMSFSDTTAALNAGVAIIYQELHLVPEMTVAEN
                     IYLGQLPHKGGIVNRSLLNYEAGLQLKHLGMDIDPDTPLKYLAIGQWQMVEIAKALAR
                     NAKIIAFDEPTSSLSAREIGNLFRVIRELRKEGRVILYVSHRMEEIFALSDAITVFKD
                     GRYVKTFTDMQQVDHDALVQAMVGRDIGDIYGWQPRSYGEERLRLDAVKALGVRTPIS
                     LAVRSGEIVGLFGLVGAGRSELMKGLFGGTQITAGQVYIDQQPIDIRKPSHAIAAGMM
                     LCPEARKAEGIIPVHSVRDNINISARRKHVLGGCVINNSWEENNADQHIRSLNIKTPG
                     AEQLIMNLSGGNQQKAILGRWLSEEMKVILLDEPTRGIDVGAKHEIYNVIYALAAQGV
                     AVLFASSDLPEVLGVADRIVVMREGEIAGELLHEQADERQALSLAMPKVSQAVA"
     gene            complement(3968..4957)
                     /gene="araF"
                     /locus_tag="OO357_002132"
                     /pseudo
     CDS             complement(3968..4957)
                     /gene="araF"
                     /locus_tag="OO357_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416414.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="arabinose ABC transporter substrate-binding
                     protein AraF"
     gene            5754..6257
                     /locus_tag="OO357_002133"
     CDS             5754..6257
                     /locus_tag="OO357_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin-like protein"
                     /protein_id="HCR5887078.1"
                     /translation="MATAGMLLKLNSQMNREFYASNLYLHLSNWCSEQSLNGTATFLR
                     AQAQSNVTQMMRMFNFMKSVGATPIVKAIDVPGEKLNSLEELFQKTMEEYEQRSSTLA
                     QLADEAKELNDDSTVNFLRDLEKEQQHDGLLLQTILDEVRSAKLAGMCPVQTDQHVLN
                     VVSHQLH"
     gene            complement(6337..6588)
                     /locus_tag="OO357_002134"
     CDS             complement(6337..6588)
                     /locus_tag="OO357_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2766 domain-containing protein"
                     /protein_id="HCR5887079.1"
                     /translation="MSQPLNADQELVSDVVACQLVIKQILDVLDVIAPVEVREKMSSQ
                     LKNIDFTHHPAAADPVTMRAIQKAIALIELKFTPQGESH"
     gene            complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="OO357_002135"
     CDS             complement(6703..6789)
                     /gene="azuC"
                     /locus_tag="OO357_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response protein AzuC"
                     /protein_id="HCR5887080.1"
                     /translation="MKLRKILKSMFNNYCKTFKDVPPGNMFR"
     gene            7052..7375
                     /locus_tag="OO357_002136"
     CDS             7052..7375
                     /locus_tag="OO357_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecR-like lipofamily protein"
                     /protein_id="HCR5887081.1"
                     /translation="MRLLILTLSLITLAGCTVTRQAHVSEVDAATGIVRLVYDQAFLQ
                     HAHTDRYVSRGIADRACQQEGYTHAIPFGQPVGNCSLFAGSLCLNTEFTLSYQCHHSA
                     FPVFL"
     gene            7546..8043
                     /gene="ftnA"
                     /locus_tag="OO357_002137"
     CDS             7546..8043
                     /gene="ftnA"
                     /locus_tag="OO357_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="non-heme ferritin"
                     /protein_id="HCR5887082.1"
                     /translation="MLKPEMIEKLNEQMNLELYSSLLYQQMSAWCSYHTFEGAAAFLR
                     RHAQEEMTHMQRLFDYLTDTGNLPRINTVESPFAEYSSLDELFQETYKHEQLITQKIN
                     ELAHAAMTNQDYPTFNFLQWYVSEQHEEEKLFKSIIDKLSLAGKSGEGLYFIDKELST
                     LDTQN"
     gene            complement(8080..8319)
                     /locus_tag="OO357_002138"
     CDS             complement(8080..8319)
                     /locus_tag="OO357_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YecH family protein"
                     /protein_id="HCR5887083.1"
                     /translation="MDSIHGHEVLNMMIESGEQYTHASLEAAIKARFGEQARFHTCSA
                     EGVTAGELVAFLAAKGKFIPSEEGFSTDQSKICRH"
     gene            8509..9720
                     /gene="tyrP"
                     /locus_tag="OO357_002139"
     CDS             8509..9720
                     /gene="tyrP"
                     /locus_tag="OO357_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine transporter TyrP"
                     /protein_id="HCR5887084.1"
                     /translation="MKNRTLGSVFIVAGTTIGAGMLAMPLAAAGVGFSVTLILLIGLW
                     ALMCYTALLLLEVYQHVPADTGLGTLAKRYLGRYGQWLTGFSMMFLMYALTAAYISGA
                     GELLASSISDWTGISMSATAGVLLFTFVAGGVVCVGTSLVDLFNRFLFSAKIIFLVVM
                     LVLLLPHIHKVNLLTLPLQQGLALSAIPVIFTSFGFHGSVPSIVSYMDGNIRKLRWVF
                     IIGSAIPLVAYIFWQVATLGSIDSTTFMGLLANHAGLNGLLQALREMVASPHVELAVH
                     LFADLALATSFLGVALGLFDYLADLFQRSNTVGGRLQTGAITFLPPLAFALFYPRGFV
                     MALGYAGVALAVLALIIPSLLTWQSRKHNPQAGYRVKGGRPALVVVFLCGIAVIGVQF
                     LIAAGLLPEVG"
     gene            complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="OO357_002140"
     CDS             complement(9782..10447)
                     /gene="yecA"
                     /locus_tag="OO357_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0149 family protein YecA"
                     /protein_id="HCR5887085.1"
                     /translation="MKTGPLNESELEWLDDILTKYNTDHAILDVAELDGLLTAVLSSP
                     QEIEPEQWLVAVWGGADYVPRWASEKEMTRFMNLAFQHMADTAERLNEFPEQFEPLFG
                     LREVDGSELTIVEEWCFGYMRGVALSDWSTLPDSLKPALEAIALHGTEENFERVEKMS
                     PEAFEESVDAIRLAALDLHAYWMAHPQEKAVQQPIKAEEKPGRNDPCPCGSGKKFKQC
                     CLH"
     gene            complement(10643..10729)
                     /locus_tag="OO357_002141"
     tRNA            complement(10643..10729)
                     /locus_tag="OO357_002141"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10693..10695),aa:Leu,seq:taa)
     gene            complement(10742..10815)
                     /locus_tag="OO357_002142"
     tRNA            complement(10742..10815)
                     /locus_tag="OO357_002142"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10781..10783),aa:Cys,seq:gca)
     gene            complement(10870..10945)
                     /locus_tag="OO357_002143"
     tRNA            complement(10870..10945)
                     /locus_tag="OO357_002143"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(10910..10912),aa:Gly,seq:gcc)
     gene            complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="OO357_002144"
     CDS             complement(11097..11645)
                     /gene="pgsA"
                     /locus_tag="OO357_002144"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HCR5887086.1"
                     /translation="MQFNIPTLLTLFRVILIPFFVLVFYLPVTWSPFAAALIFCVAAV
                     TDWFDGFLARRWNQSTRFGAFLDPVADKVLVAIAMVLVTEHYHSWWVTLPAATMIARE
                     IIISALREWMAELGKRSSVAVSWIGKVKTTAQMVALAWLLWRPNIWVEYVGIALFFVA
                     AVLTLWSMLQYLSAARADLLDQ"
     gene            complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="OO357_002145"
     CDS             complement(11702..13534)
                     /gene="uvrC"
                     /locus_tag="OO357_002145"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310678.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HCR5887087.1"
                     /translation="MSDQFDAKAFLKTVTSQPGVYRMYDAGGTVIYVGKAKDLKKRLS
                     SYFRSNLASRKTEALVAQIQQIDVTVTHTETEALLLEHNYIKLYQPRYNVLLRDDKSY
                     PFIFLSGDTHPRLAMHRGAKHAKGEYFGPFPNGYAVRETLALLQKIFPIRQCENSVYR
                     NRSRPCLQYQIGRCLGPCVEGLVSEEEYAQQVEYVRLFLSGKDDQVLTQLISRMETAS
                     QNLEFEEAACIRDQIQAVRRVTEKQFVSNTGDDLDVIGVAFDAGMACVHVLFIRQGKV
                     LGSRSYFPKVPGGTELSEVVETFVGQFYLQGSQMRTLPGEILLDFNLSDKTLLADSLS
                     ELAGRKINVQTKPRGDRARYLKLARTNAATALTSKLSQQSTVHQRLTALASVLKLPEV
                     KRMECFDISHTMGEQTVASCVVFDANGPLRAEYRRYNITGITPGDDYAAMNQVLRRRY
                     GKAIDDSKIPDVILIDGGKGQLAQAKNVFAELDVSWDKNHPLLLGVAKGADRKAGLET
                     LFFEPEGEGFSLPPDSPALHVIQHIRDESHDHAIGGHRKKRAKVKNTSSLETIEGVGP
                     KRRQMLLKYMGGLQGLRNASVEEIAKVPGISQGLAEKIFWSLKH"
     gene            complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="OO357_002146"
     CDS             complement(13531..14187)
                     /gene="uvrY"
                     /locus_tag="OO357_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UvrY/SirA/GacA family response regulator
                     transcription factor"
                     /protein_id="HCR5887088.1"
                     /translation="MINVLLVDDHELVRAGIRRILEDIKGIKVVGEASCGEDAVKWCR
                     TNAVDVVLMDMSMPGIGGLEATRKIARSTADVKIIMLTVHTENPLPAKVMQAGAAGYL
                     SKGAAPQEVVSAIRSVYSGQRYIASDIAQQMALSQIEPEKTESPFASLSERELQIMLM
                     ITKGQKVNEISEQLNLSPKTVNSYRYRMFSKLNIHGDVELTHLAIRHGLCNAETLSSQ
                     "
     gene            14483..14659
                     /locus_tag="OO357_002147"
     CDS             14483..14659
                     /locus_tag="OO357_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887089.1"
                     /translation="MIKIFIGHYINVFYSTADITLKKQPLLFLAKLMVYSAALTFFTT
                     NFHCNMTRKINEYA"
     gene            14646..14870
                     /locus_tag="OO357_002148"
     CDS             14646..14870
                     /locus_tag="OO357_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2594 family protein"
                     /protein_id="HCR5887090.1"
                     /translation="MSTPDFSTAENNQELANEVSCLKAMLTLMLQAMGQADAGRVMLK
                     MEKQLALIEDETQAAVFSKTVKQIKQAYRQ"
     gene            complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="OO357_002149"
                     /pseudo
     CDS             complement(14903..15523)
                     /gene="sdiA"
                     /locus_tag="OO357_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310681.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator SdiA"
     gene            complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="OO357_002150"
     CDS             complement(15753..16505)
                     /gene="yecC"
                     /locus_tag="OO357_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine ABC transporter ATP-binding protein
                     YecC"
                     /protein_id="HCR5887091.1"
                     /translation="MSAIEVKNLVKKFHGQTVLHGIDLEVKPGEVVAIIGPSGSGKTT
                     LLRSINLLEQPEAGTITVGDITIDTARSLSQQKSLIRQLRQHVGFVFQNFNLFPHRTV
                     LENIIEGPVIVKGEPKEEATARARELLAKVGLAGKETSYPRRLSGGQQQRVAIARALA
                     MRPEVILFDEPTSALDPELVGEVLNTIRQLAQEKRTMVIVTHEMSFARDVADRAIFMD
                     QGRIVEQGAAKALFADPQQPRTRQFLEKLLLQ"
     gene            complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="OO357_002151"
     CDS             complement(16502..17170)
                     /gene="tcyL"
                     /locus_tag="OO357_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter permease"
                     /protein_id="HCR5887092.1"
                     /translation="MQESIQLVIDSLPFLLKGAGYTLQLSIGGMFFGLLLGFILALMR
                     LSPIWPVRWLARFYISIFRGTPLIAQLFMIYYGLPQFGIELDPIPSAMIGLSLNTAAY
                     AAETLRAAISSIDKGQWEAAASIGMTPWQTMRRAILPQAARVALPPLSNSFISLVKDT
                     SLAATIQVPELFRQAQLITSRTLEVFTMYLAASLIYWIMATVLSTLQNHFENQLNRQE
                     REPK"
     gene            complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="OO357_002152"
     CDS             complement(17185..18171)
                     /gene="dcyD"
                     /locus_tag="OO357_002152"
                     /EC_number="4.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase"
                     /protein_id="HCR5887093.1"
                     /translation="MPLHNLTRFPRLEFIGAPTPLEYLPRFSDYLGREIFIKRDEVTP
                     MAMGGNKLRKLEFLAADALREGADTLITAGAIQSNHVRQTAAVAAKLGLHCVALLENP
                     IGTTAENYLTNGNRLLLDLFNTQIEMCDALTDPNAQLEELATRVEAQGFRPYVIPVGG
                     SNALGALGYVESALEIAQQCEGAVNISSVVVASGSAGTHAGLAVGLEHLMPESELIGV
                     TVSRSVADQLPKVVNLQQAIAKELELTASAEILLWDDYFAPGYGVPNDEGMEAVKLLA
                     RLEGILLDPVYTGKAMAGLIDGISQKRFKDEGPILFIHTGGAPALFAYHPHV"
     gene            complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="OO357_002153"
     CDS             complement(18276..19076)
                     /gene="tcyJ"
                     /locus_tag="OO357_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5887094.1"
                     /translation="MKLAHLGRQALMGVMAVALVAGMSVKSFADEGLLNKVKESGTLL
                     VGLEGTYPPFSFQGDDGKLTGFEVEFAQQLAKHLGVEASLKPTKWDGMLASLDSKRID
                     VVINQVTISDERKKKYDFSTPYTISGIQALVKKGNEGTIKTAADLKGKKVGVGLGTNY
                     EEWLRQNVQGVDVRTYDDDPTKYQDLRVGRIDAILVDRLAALDLVKKTNDTLAVTGEA
                     FSRQESGVALRKGNEDLLKAVNDAIAEMQKDGTLQALSEKWFGADVTK"
     gene            complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="OO357_002154"
     CDS             complement(19164..19715)
                     /gene="fliZ"
                     /locus_tag="OO357_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliZ"
                     /protein_id="HCR5887095.1"
                     /translation="MMVQHLKRRPLSRYLKDFKHSQTHCAHCRKLLDRITLVRDGKIV
                     NKIEISRLDTLLDEKGWQTEQQSWAALCRFCGDLHCKTQSDFFDIIGFKKFLFEQTEM
                     SPGTVREYVVRLRRLGNHLHEQNISLDQLQDGFLDEILAPWLPTTSTNNYRIALRKYQ
                     HYQRQTCTGLVQKSSSLPASDIY"
     gene            complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="OO357_002155"
                     /pseudo
     CDS             complement(19761..20480)
                     /gene="fliA"
                     /locus_tag="OO357_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310688.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor FliA"
     gene            complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="OO357_002156"
     CDS             complement(20645..22297)
                     /gene="fliC"
                     /locus_tag="OO357_002156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellin FliC"
                     /protein_id="HCR5887096.1"
                     /translation="MAQVINTNSLSLITQNNINKNQSALSSSIERLSSGLRINSAKDD
                     AAGQAIANRFTSNIKGLTQAARNANDGISVAQTTEGALSEINNNLQRIRELTVQASTG
                     TNSDSDLDSIQDEIKSRLDEIDRVSGQTQFNGVNVLAKDGSMKIQVGANDGQTITIDL
                     KKIDSDTLGLNGFNVNGGGAVANTAASKADLVAANATVVGNKYTVSAGYDAAKASDLL
                     AGVSDGDTVQATINNGFGTAASATNYKYDSASKSYSFDTTTASAADVQKYLTPGVGDT
                     AKGTITIDGSAQDVQISSDGKITASNGDKLYIDTTGRLTKNGSGASLTEASLSTLAAN
                     NTKATTIDIGGTSISFTGNSTTPDTITYSVTGAKVDQAAFDKAVSTSGNNVDFTTAGY
                     SVNGTTGAVTKGVDSVYVDNNEALTTSDTVDFYLQDDGSVTNGSGKAVYKDADGKLTT
                     DAETKAATTADPLKALDEAISSIDKFRSSLGAVQNRLDSAVTNLNNTTTNLSEAQSRI
                     QDADYATEVSNMSKAQIIQQAGNSVLAKANQVPQQVLSLLQG"
     gene            22563..23969
                     /gene="fliD"
                     /locus_tag="OO357_002157"
     CDS             22563..23969
                     /gene="fliD"
                     /locus_tag="OO357_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar filament capping protein FliD"
                     /protein_id="HCR5887097.1"
                     /translation="MASISSLGVGSGLDLSSILDSLTAAQKATLTPISNQQSSFTAKL
                     SAYGTLKSALTTFQTANTALSKADLFSATSTTSSTTAFSATTAGNAIAGKYTISVTHL
                     AQAQTLTTRTTRDDTKTAIATSDSKLTIQQGDDKDPITIDISAANSSLSGIRDAINNA
                     KAGVSASIINVGNGEYRLSVTSNDTGLDNAMTLSVSGDDALQSFMGYDASASSNGMEV
                     SVAAQNAQLTVNNVAIENSSNTISDALENITLNLNDVTTGNQTLTITQDTSKVQTAIK
                     DWVNAYNSLIDTFSSLTKYTAVDAGADSQSSSNGALLGDSTLRTIQTQLKSMLSNTVS
                     SSSYKTLAQIGITTDPSDGKLELDADKLTAALKKDASGVGALIVGDGKKTGITTTIGS
                     NLTSWLSTTGIIKAATDGVSKTLNKLTKDYNAASDRIDAQVARYKEQFTQLDVLMTSL
                     NSTSSYLTQQFENNSNSK"
     gene            23994..24404
                     /gene="fliS"
                     /locus_tag="OO357_002158"
     CDS             23994..24404
                     /gene="fliS"
                     /locus_tag="OO357_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar export chaperone FliS"
                     /protein_id="HCR5887098.1"
                     /translation="MYAAKGTQAYAQIGVESTVMSASQQQLVTMLFDGVLSALVRARL
                     FMQDNNQQGKGVSLSKAINIIENGLRVSLDEESKDELTQNLIALYSYMVRRLLQANLR
                     NDVSAVEEVEALMRNIADAWKESLLSPSSIQDPV"
     gene            24404..24769
                     /gene="fliT"
                     /locus_tag="OO357_002159"
     CDS             24404..24769
                     /gene="fliT"
                     /locus_tag="OO357_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis regulatory protein FliT"
                     /protein_id="HCR5887099.1"
                     /translation="MNNAPHLYFAWQQLVEKSQLMLRLATEEQWDELIASEMAYVNAV
                     QEIAHLTEEVAPSTTMQEQLRPMLHLILDNESKVKQLLQIRMDELAKLVGQSSVQKSV
                     LSAYGDQGGFVLAPQDNLF"
     gene            24847..26334
                     /gene="amyA"
                     /locus_tag="OO357_002160"
     CDS             24847..26334
                     /gene="amyA"
                     /locus_tag="OO357_002160"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5887100.1"
                     /translation="MRNPTLLQCFHWYYPEGGKLWPELAERADGFNDIGINMVWLPPA
                     YKGASGGYSVGYDSYDLFDLGEFDQKGSIPTKYGDKAQLLAAIDALKRNDIAVLLDVV
                     VNHKMGADEKEAIRVQRVNADDRTQIDEEIIECEGWTRYTFPARAGQYSQFIWDFKCF
                     SGIDHIEHPDEDGIFKIVNDYTGEGWNDQVDDELGNFDYLMGENIDFRNHAVTEEIKY
                     WARWVMEQTQCDGFRLDAVKHIPAWFYKEWIEHVQEVAPKPLFIVAECWSHEVDKLQT
                     YIDQVEGKTMLFDAPLQMKFHEASRMGRDYDMTQIFTGTLVEADPFHAVTLVANHDTQ
                     PLQALEAPVEPWFKPLAYALILLRENGVPSVFYPDLYGAHYEDVGGDGQTYPIDMPII
                     EQLDELILARQRFAHGVQTLFFDHPNCIAFSRSGTDEYPGCVVVMSNGDDGEKTIHLG
                     ENYGNKTWRDFLGNRQESVVTDENGEATFFCNGGSVSVWVIEEVI"
     gene            complement(26368..26781)
                     /locus_tag="OO357_002161"
     CDS             complement(26368..26781)
                     /locus_tag="OO357_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5887101.1"
                     /translation="MKKLAIAGALMLLAGCAEVENYNNVVKTPAPDWLAGYWQTKGPQ
                     RALVSPEAIGSLIVTKEGDTLDCRQWQRGIAVPGKLTLMSDDLTNVTVKRELYEVERD
                     GNTIEYDGMTMERVDRPTAECAAALDKAPLPTPLP"
     gene            26968..28173
                     /gene="yedE"
                     /locus_tag="OO357_002162"
     CDS             26968..28173
                     /gene="yedE"
                     /locus_tag="OO357_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium metabolism membrane protein YedE/FdhT"
                     /protein_id="HCR5887102.1"
                     /translation="MSWQQFKHAWLIKFWAPIPAVIAAGILSTYYFGITGTFWAVTGE
                     FTRWGGQLLQLFGVHAEEWGYFKIIHLEGSPLTRIDGMMILGMFGGCFAAALWANNVK
                     LRMPRSRIRIMQAIIGGIIAGFGARLAMGCNLAAFFTGIPQFSLHAWFFAIATAIGSW
                     FGARFTLLPIFRIPVKMQKVSAASPLTQKPDQARRRFRLGMLVFFGMLGWALLTAMNQ
                     PKLGLAMLFGVGFGLLIERAQICFTSAFRDMWITGRTHMAKAIIIGMAVSAIGIFSYV
                     QLGVEPKIMWAGPNAVIGGLLFGFGIVLAGGCETGWMYRAVEGQVHYWWVGLGNVIGS
                     TILAYYWDDFAPALATDWDKINLLKTFGPMGGLLVTYLLLFAALMLIIGWEKRFFRRE
                     APQTAKEIA"
     gene            28170..28403
                     /gene="yedF"
                     /locus_tag="OO357_002163"
     CDS             28170..28403
                     /gene="yedF"
                     /locus_tag="OO357_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase-like selenium metabolism
                     protein YedF"
                     /protein_id="HCR5887103.1"
                     /translation="MKNIVPDYRLDMVGEPCPYPAVATLEAMPQLKKGEILEVVSDCP
                     QSINNIPLDARNHGYTVLDIQQDGPTIRYLIQK"
     gene            28510..29180
                     /locus_tag="OO357_002164"
                     /pseudo
     CDS             28510..29180
                     /locus_tag="OO357_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016245363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase"
     gene            complement(29220..>29658)
                     /locus_tag="OO357_002165"
     CDS             complement(29220..>29658)
                     /locus_tag="OO357_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5887104.1"
                     /translation="SKNHVMIVIEDLKVSNMSKSAAGTVSQPGRNVRAKSGLNRSILD
                     QGWYEMRRQLEYKQLWRGGQVLAVPPAYTSQRCACCGHTAKENRLSQSKFRCQVCGYT
                     ANADVNGARNILAAGHAVLACGEMVQSGRSLKQEPTEMIQATA"
BASE COUNT         7372 a         7666 c         7059 g         7561 t
ORIGIN      
        1 caatgcgatt attcacccca cgtttgcgta attcatgcgc aaagggcaac aggtgataat
       61 cgtggatcca gataatgtca tcgtcttgca acagcggcag taatttatct gccagcaacg
      121 catttacgcg tagatagccg tcccaggcag gacgctgaaa ttgcaccaga tcgagccgat
      181 aatgaaaagc gggccagaga acggcattgg agaattggtt gtagtattcg tcaaggtcct
      241 gttcgctgag gttaaaagag gcccacgtaa tgttaccttt tttagcggct gatcctcatt
      301 ccctgtttca ccactccagc caaaccacag tccgcctgcg gctttcagtg cccccagtat
      361 gccaacggca aggccaccgg cactggcggc gtgctcgtct ggtggtgcaa tccggttaga
      421 tactacgact aaacgactca tagtcatcac tcctgttatt ttctcttttt tgttgtaatg
      481 cggcggttat catttcaagc cagctccaga catccggcac acccgccagt cgccatgatg
      541 cctgagttgc acctgtgcca atttttactg acattccgcc cagtcggtta acgactgcga
      601 agccagattc atcggttaaa tcatcgccca gaaatacggg cgttcgcccg ataaaaggag
      661 cttcctgcat aaaggctgca atcgcttcac ctttactggt acctctcggt ttgatctcga
      721 caacacactt tccctgctgt aacgccattt gtggccagat ctgagtaata cgttgcgcta
      781 atgtcattaa tgcgtcttca tgctgcggag cctgacgata atgcagcgca aaagccatcc
      841 ctttcgcctc cagctccgcg ccgggatact gagcgatgac cgtatgcagt tgcacgctaa
      901 tatcacgcgc aatcgcatcc ggcagatgaa cgatatgtgt tttaccattg atgtcacggc
      961 gctccgcccc atgcacgccc gctaacggga agcgataagg ttttgccagt gcgtcaagct
     1021 ccaccattga gcgccctgat atcaatgcca atgcaccatc acttgcggtt gccagtagct
     1081 gtagtccttg cagaatattg tcaggcacga cgacctgatc gggatgcggt ttgatttccg
     1141 ccagcgttcc atcaagatca aaaaaccagg catatttcgc ggatagttca ggggtttcgg
     1201 ttaacggttc tgtcacccgg ctctcctcct tctcttttct tattgtgttg cgcagaccgc
     1261 gctttacaga catgtaagta tagacagtgt gacggaggtc gccatttgca acgaagaaca
     1321 gccaaccgtt gtggcggctg gctgtggtgg gaaaaaacgt taagttgttg tggaaaaaag
     1381 catcagacag tgcgcttcgc tttttgcttg taacggtcga agatcaccgc tgccagcagg
     1441 attaagccgc gaaccacgta ctgcgcgaaa ggagaaatat taagcaggtt catggcgttt
     1501 tccacggtgc ctaaaattaa gatacccgcc accacatatg agatttttcc gatgccacct
     1561 ttcagagaaa cgccacctaa aacgcaggcg gagataacaa taagctcata accaatcgac
     1621 gtcattggct gcccactagt catacgtgaa gccagaataa ttccggctat cgcagatacc
     1681 aggccagaga gaacaaagat aataattttg gtgcgaacaa ccggtacacc cgccagacgc
     1741 gcagcctctt cgttcccgcc aatcgccagg gtattacgac caaaggtggt tttattcagc
     1801 agcaaaccaa agataatcag acacgcgacg gtgagccaga ttggcgcagg cagaccgaac
     1861 cagttagcgt aaccaagggc aaagaagctt tcatcttcga taccgaccgc tttaccgtct
     1921 gaaatgatgt acgccagacc tcgaacaatc tgcatcgttg ccagtgtcgt gatcagagca
     1981 tttattttca gtttggcgat aacaaagcca ttgaccaggc cacagagaac gcccagcaac
     2041 aaacccgctg ccacgccaat ccacaggctt tcagtcaggt tgataaccac cgccgtggtg
     2101 acacccgcac aggcaattac ggaggcgaca gaaaggtcaa agtcaccgga agcaaggcag
     2161 aacaacatgc cacacgccac catccccgac atggaaattg ccaggcccaa ccctttcata
     2221 ttaatgaagg tggcaaaatt tgggacaaaa atggcacagg cgataaagag caccgcaaac
     2281 accaccagca tgccgtactg atcccagata cgcccgaagc tgaatgacga cttaggtgcg
     2341 ccagaccccg atgtagaaac agaagacatc atactctcct tactcaggca actgcctggc
     2401 tgactttagg catcgcaagg ctcagtgcct gacgctcatc tgcctgctcg tgtaacaatt
     2461 caccggcgat ttcgccttcc cgcatcacca caatccggtc ggcaacgccg aggacttcag
     2521 gtaagtcgct ggaggcaaac agcaccgcca cgccctgcgc cgccagcgca taaattacgt
     2581 tatatatttc gtgcttagcg ccaacatcaa tgccgcgcgt aggttcatcc agcaaaatga
     2641 ccttcatctc ttccgataac cagcggccca gaatggcttt ttgctgattt ccgcctgaga
     2701 gattcatgat cagttgctcc gcgcccggcg ttttgatgtt gagcgaacga atgtgctgat
     2761 cggcattgtt ttcttcccaa ctgttgttga ttacacaacc gccgagcaca tgtttgcgtc
     2821 tggcactgat gttgatattg tcgcgaacgg agtgcacggg aataatgcct tccgctttgc
     2881 gagcttccgg gcagagcatc atgcctgcgg caatggcgtg gctcggttta cgaatatcga
     2941 tcggctgttg gtcgatataa acctgaccgg cggtgatttg cgtcccgcca aacaagcctt
     3001 tcattaattc gctacgcccc gcccctacca gaccaaacag cccaacaatt tcaccactgc
     3061 gaaccgccag acttattggc gtacgcacgc ctagtgcttt cacagcatca agacgcaggc
     3121 gctcctcgcc ataactacga ggttgccagc cgtagatatc gccaatgtcg cgcccgacca
     3181 tcgcctgcac cagcgcgtcg tggtcaacct gctgcatatc ggtaaaggtt ttgacataac
     3241 gtccatcttt aaagacagtg atggcatcgc tgagggcaaa tatttcttcc atacggtgag
     3301 aaacgtataa gatcacccgc ccctcttttc gcagttcacg aataacgcgg aaaagattgc
     3361 cgatttcgcg ggcagaaagg gagctggttg gctcatcaaa ggcgataatt ttagcgttac
     3421 gcgccagcgc tttggcgatt tcaaccatct gccactgacc aatggcgaga tatttcagcg
     3481 gcgtgtccgg gtcaatatcc ataccaagat gtttaagttg taaacccgcc tcataattca
     3541 gcaatgagcg attcacaatg ccgcctttat gcggcagctg gccgagatag atgttttccg
     3601 cgacggtcat ttccggcacg agatgcagtt cctggtaaat aatcgccaca cccgcgttaa
     3661 gtgctgcggt cgtgtcggaa aaggacattt cctgcccatt aatcactaca gaacccgtgg
     3721 ttggcgcata gttgccgctg aggattttta agagagttga ttttcctgcg ccattttcac
     3781 ccatcaacgc atgaacctga ccggcatagc agtcaaaact aatatccgtc agcgccttaa
     3841 cgccgggaaa cgttttaccg atgccgcgaa atgagagata cggggtagac tgttgcataa
     3901 cgtctccgtg aatcactggt cgttcacacc ccctctgcat catgcagagg ggaatttttc
     3961 cggcaaatta cttaccgcct aaaccttttt tctccagttc ttctttaaag ttgtcacgcg
     4021 tgatcagtac cacgtctgta acttcggtaa attttggcgt ttcaacgtct tttgctaccc
     4081 agttgtaaag catttcgctg gatttatagc catgtacgtc cgggcttggc agcagggaac
     4141 cgtagaagcc ggttgcctgt gctttagaca gttcgcttac tgcatccaca ccgttaatac
     4201 caataccgat gatatcggct gctttaaagc cctgaccttc ggttgcacgt acgccgccaa
     4261 gtacggtgct gtcgttcata ccgacgatca gccaatgttt aacttccgga tgttgaacca
     4321 gcattgagtt ggcagcgtca aatgcccccg ggatgtcgtt agatttggta ggtacctgat
     4381 aaatttgttt ttccgggaat cctgcagctt tcagtgcgtc catagaaccc gttgtacggc
     4441 ggcgggcggt atccagttcg ttggcggtaa tcgccatcac tgcgctttct ttgacatccc
     4501 agccacgttt ctgcatctct ttatacagtt cctagccctg acgttcgcca attttagtcg
     4561 ccgccatcat caccagagga acggtatcca ttggcttacc tttggcgtta acaaactggt
     4621 catccactgc aatgactttc atatcgtagc cacgcgcttt cgcgacgatg gcagagccga
     4681 gtttcgggtc cggagtacaa ataacgaaac cttttgcgcc actggctgcc aggctgtcga
     4741 tcgcgttcaa tgttttttcg ccatccggca cggcaatctt aataacctca aaccctaaat
     4801 ccttcccggc tttatcggca aacttccatt cggtctggaa ccacggctct tccggttgct
     4861 tcaccagaaa accgagcttc aggttctccg ccatagcgga ttgtgacata acggctgcca
     4921 gaccaatggc tgccagggct ttagtaaatt tgtgcatggt tctctccagc tttagtgtcg
     4981 ttttgtgtag ggcaaaaacg aatgacattc gttaaattaa tcggaaaaca aagcattacc
     5041 ttttatctaa aagataagtg accgtgttga catagtttta gcgagaaatt aattctccat
     5101 aggagagcaa tatcacatcg cagaattaca gtgagaacgt gcataaattt agcgggaaaa
     5161 gacataagga aaagccaatt tgtcagacaa attgttgaat gcacagcaga ttaatccata
     5221 agattagcct ggaaatcctt gttgtcttcg gtacccatgc gggatgtctt ctttttaacc
     5281 agtcaatagg ccgcattacc tggcgttgag tttttgaaat ggtgtaataa ccacaactca
     5341 aagatgtgga aaatgcgcgt cattcatttc gccattaatt atcactgtgc tcattaatta
     5401 acagaacacg tataatgaga gccatctcgc aaaaatgaaa aaacgtttta taaaatcatc
     5461 acttcatcat gaattcaaat tcatcgatta atatcaacaa gatacaaaaa gcactatcat
     5521 taaaattcat tgcatttaca ttgatttcac caatgaaatg taaaaatata taaacttgat
     5581 gatttaagca ttttcttata accgttcaga cgttattctt atttcagatc atcgtcagaa
     5641 ttgactccac gatcacattt cggaccggca gaaaggaatt attctgcaaa cagtaattat
     5701 ggtgttttga tttatcttgc acctctccac ttctggatat aaggatatta ggtatggcaa
     5761 ccgctggaat gcttctcaaa ctcaactctc aaatgaaccg cgagttttac gcatccaatc
     5821 tctaccttca cctgagtaac tggtgttctg aacagagtct gaacggcacc gccactttcc
     5881 ttcgcgccca ggcacagagt aatgtgaccc aaatgatgcg catgtttaac tttatgaaga
     5941 gtgtcggcgc tacccccatc gttaaagcca ttgatgttcc aggtgaaaaa ctgaactctc
     6001 tggaagaact gttccaaaaa acgatggaag aatacgagca acgttcaagc actctggcgc
     6061 agttagccga tgaagcgaaa gaactgaatg atgattcaac cgtcaatttc ctgcgcgatc
     6121 tggaaaaaga acagcagcat gatggcctgt tgctgcaaac cattcttgat gaagtgcgca
     6181 gtgcgaaact tgcgggtatg tgccctgtgc agaccgacca acatgttctg aatgtcgtgt
     6241 cacaccagct gcattgatca tacatcggcg ctaatacatt gcgccgatga agtttttgag
     6301 aaaccgctgc ctcatctgtt tgaagcagcg gtttttttaa tgggattcac cctgtggggt
     6361 aaacttgagt tcaatcagcg cgatggcttt ttggattgcc cgcatggtga ccgggtctgc
     6421 ggcagcggga tgatgagtaa agtcgatatt cttcagctga ctggacattt tttcacgaac
     6481 ttcaacgggc gcgattacat cgagaacatc cagaatttgt ttgataacca actggcaagc
     6541 taccacatca gaaaccagtt cctgatcggc attcagcggc tgggacatcg taaactcctg
     6601 atagcatttt gaaagccgtt atagtagcgg cttcacatct tcagcgatag tcacatccac
     6661 cgtcatcagg acacaaaaaa acctgccgga gcaggttttt tgttatcgga acatattgcc
     6721 tggcggtacg tctttgaacg tcttgcaata gttattgaac atacttttca ggattttgcg
     6781 cagtttcatc gcggcactcc gaccatttgt tatacaggtg ttattgtctt tgcgcttata
     6841 atatgacaac catcacaaaa atcaatcttt atgtgataca aatcacataa ataccccttt
     6901 aatgttataa aaatgataat caaaaaacag cccccctatt tctgacacct acagatggca
     6961 agaaatagcg cctgccaggc gtcttttccg gccattgtcg cagcgctgta acgcgtaaaa
     7021 tagtgctttc tcttactctt ctggctggac catgagactt ctgattctga ctctttcatt
     7081 aataacgctg gcggggtgta cggttactcg ccaggcccat gtgagcgaag ttgacgcggc
     7141 aacaggtatt gtacggttgg tttatgacca ggcttttttg cagcatgccc ataccgatcg
     7201 ttatgtcagt cgcgggatcg ccgatcgcgc atgccagcag gaaggctata cccacgcgat
     7261 cccctttggt cagccagtag gcaactgtag cctttttgcg ggttccttat gtttgaatac
     7321 ggaattcact ttgtcatatc agtgccatca ttccgccttc cctgtctttc tctaaatatt
     7381 ttaatcagcg aggggatctt cgctgattaa agaaatagcg gtaatgcgtt ttattcccat
     7441 tcgtattttt aataattgaa gtttatattt taccttttgc aaataataaa ataacaaatt
     7501 atagtgacgc cacagcaaca aatataacct ttgtggagca ctatcatgct gaaaccagaa
     7561 atgattgaaa aacttaatga gcagatgaac ctggaactgt actcttcact gctttatcag
     7621 caaatgagcg cctggtgcag ctatcatacc ttcgaaggtg ctgccgcgtt cctgcgccgt
     7681 cacgcccagg aagagatgac gcatatgcag cgtctgtttg attacctgac tgataccggc
     7741 aatttaccgc gtattaatac cgttgaatct ccgtttgctg aatattcctc acttgatgaa
     7801 ttattccagg aaacctataa acacgaacaa ttaatcaccc agaaaattaa cgaactggct
     7861 catgctgcaa tgaccaatca ggactaccca acatttaatt tcctgcagtg gtatgtttct
     7921 gagcagcatg aagaagagaa actgttcaaa tcgattattg ataaattaag cctggcaggc
     7981 aaaagcggcg aaggtctgta ttttatcgac aaagaactct ctaccctcga cacacaaaac
     8041 taatgctctc cggcggcagg tttctgccgc cgtttcaaat cagtgacggc aaatcttact
     8101 ctgatcggtc gaaaaacctt cttctgaagg tataaatttg ccttttgctg ccagaaacgc
     8161 taccagctct cccgctgtca ccccttctgc cgagcacgtg tgaaaacgtg cctgttcacc
     8221 aaaacgcgct ttaatcgcag cttccagact ggcatgcgta tattgctcgc ctgattcaat
     8281 catcatattt aacacttcat gaccgtgaat agagtccatc gttcctcctc aaaaaaagcc
     8341 tagcgtagcg attaccgctt atgaagactt tgcgccagcg caggactgaa tgcttttatt
     8401 gtacatttat atttacacaa tatgtaacgt cggtttgacg aagcagccgt tatgccttaa
     8461 cctgcgccgc agatatcact cataaagatc gtcaggacag aagaaagcgt gaaaaacaga
     8521 accctgggaa gtgtttttat cgtggcggga accacaattg gcgcaggcat gctggcaatg
     8581 ccgctggctg cggccggtgt tggttttagc gttacgttaa tcttgttgat tgggctttgg
     8641 gcgttgatgt gctacacggc gctattactg ctggaggtgt accagcatgt tccggcagat
     8701 accggtctgg gcacgctggc aaaacgctat ctgggacgct acggtcaatg gctgacgggc
     8761 ttcagtatga tgttcttaat gtatgctctg actgcagcat acatcagcgg tgccggtgaa
     8821 ttgttggcct ccagcatcag cgactggaca ggtatttcaa tgtcggcaac cgctggcgtg
     8881 ctgttgttca cttttgttgc cggtggcgtg gtttgtgttg gaacatcgct ggtcgattta
     8941 tttaaccgtt ttctgttcag cgccaaaatt atttttctgg tggtgatgct ggtattgctg
     9001 ctaccgcata ttcacaaagt gaatctttta accctgccgt tgcaacaggg gctggcactg
     9061 tctgcaatcc cggtgatttt tacgtcgttt ggttttcacg gtagcgtgcc gagtattgtc
     9121 agctatatgg atggcaacat tcgtaagcta cgctgggtgt ttataatcgg tagtgccatc
     9181 cccctggtgg catatatttt ctggcaggtg gcgacgcttg gcagcattga ttcaacaacc
     9241 tttatgggat tgctggctaa tcatgctgga ttaaacgggc tgttacaggc gttacgcgaa
     9301 atggtggcct ctccgcatgt tgagctggcg gtgcatttat ttgctgattt agccctcgcc
     9361 acgtcatttc tcggcgttgc gttaggctta tttgattatc tggctgattt gtttcagcgt
     9421 tcaaataccg ttggtggacg attgcaaact ggtgcaatta cctttctgcc gccgttggcg
     9481 tttgcactgt tttatccacg aggatttgtg atggcgctgg gttacgccgg tgtggcgctg
     9541 gcggtactgg cattgattat cccttcacta ttgacctggc aaagcagaaa gcacaatcct
     9601 caggcgggtt accgggtcaa aggtggtcgt ccggcgctgg tggtggtgtt tctctgtggt
     9661 attgctgtga ttggcgtgca atttttgatt gcggcagggt tgttaccaga agtggggtga
     9721 tcagatagcc tcaaattcct tattgggtgc cagaattaac gctgacaccc aatgtgacct
     9781 cttaatgcag gcagcactgc ttaaatttct taccactgcc gcacggacaa ggatcgttac
     9841 gccccggttt ctcttctgct ttgatcggtt gctgaacagc tttttcctgc ggatgcgcca
     9901 tccagtatgc atgtaaatca agcgccgcca gtcgaatggc atctacgctc tcttcaaacg
     9961 cttctggcga cattttctct acccgctcga agttttcctc agtaccgtgc agcgcaatcg
    10021 cctccagcgc tggttttaaa gaatcgggca acgttgacca gtcagaaagt gccacgcccc
    10081 gcatatagcc aaagcaccac tcctcaacaa tcgtcagctc gctgccatca acttctcgca
    10141 agccgaataa cggctcaaac tgctccggga attcgttcag acgctctgcg gtatcggcca
    10201 tatgttgaaa agccaggttc ataaagcgcg tcatctcttt ctctgacgcc caacgcggca
    10261 catagtcagc cccaccccac acggcaacca gccactgttc tggttcaatc tcttgcggag
    10321 aactcaacac cgccgtcaat aaaccgtcca gctccgccac atcaaggatg gcgtggtcag
    10381 tgttgtattt ggtcagaata tcgtccagcc attccaactc actttcgttt aacggtcccg
    10441 ttttcatacg cttttccttg tggatctcaa ctcgccagca cctatcttac atgccggtcc
    10501 gtatcagaga tactttttga gtggctttgc cggtgattaa aaattaagga gggtgtaacg
    10561 acaagttgca ggcacaaaaa aaccacccga aggtggtttc acgacactgc ttattacttt
    10621 gattttattc ttatctttcc catggtaccc ggagcgggac ttgaacccgc acagcgcgaa
    10681 cgccgaggga ttttaaatcc cttgtgtcta ccgattccac catccgggct cgggaagaaa
    10741 gtggaggcgc gttccggagt cgaaccggac tagacggatt tgcaatccgc tacataaccg
    10801 ctttgttaac gcgccaaatt cttcaggcct ttcagccaga catccgcttg acgccgatgt
    10861 cttttaaact ggagcgggaa acgagactcg aactcgagac ctcgaccttg gcaaggtcgt
    10921 gctctaccaa ctgagctatt cccgcattca tcaagcaatc agttaatcac ttgattttat
    10981 tatcgtctgg caatcagtgc cgccgttcga tgcgttgcat tctacttacc tggcgcgatg
    11041 agtcaacgat atttttcacc acttttgatc gtttgctgaa aattacgccg aaacgatcac
    11101 tgatcaagca aatctgcacg cgcagcgctc aaatattgca acattgacca cagagtcagt
    11161 accgcagcca caaagaaaag tgcaataccg acgtactcaa cccaaatgtt cggacgccac
    11221 agcagccagg ccaacgccac catctgggca gtggttttca ctttcccaat ccaggagacg
    11281 gccacgctac tgcgtttacc caactccgcc atccattcgc gtagcgcaga aataataatt
    11341 tcacgggcga tcatcgttgc cgccggtaat gtcacccacc agctgtgata atgctcggtt
    11401 accagcacca tggcgatagc cacgagaact ttatctgcca cagggtcaag gaaagcacca
    11461 aaccgggtac tctggttcca gcggcgtgcc agaaaaccat cgaaccagtc agtcaccgcc
    11521 gcgacgcaga aaatgagcgc ggcggcaaac ggcgaccagg tgacaggcag ataaaagacc
    11581 aatacaaaga atgggataag gatgacacgg aacagtgtaa gcaacgtagg gatattaaat
    11641 tgcataatga cgggtaacta tctgttgtca gtaagattac ccctatgttg ctacagagac
    11701 atcaatgttt caacgaccag aagatctttt ctgccagacc ttgcgaaata cccggcactt
    11761 ttgcaatttc ctcgacgctg gcgttacgta aaccttgcaa accgcccata tatttcaaca
    11821 acatttgccg acgttttggc ccgacgcctt caatggtttc cagggaactg gtatttttga
    11881 ccttcgcccg ttttttacgg tgcccgccaa tcgcgtgatc atgtgattca tcgcgaatat
    11941 gctggataac atgcagcgcg ggtgaatctg gcggcaaact aaatccctca ccttccggct
    12001 caaagaacag cgtttccagt ccagccttac gatctgctcc tttggcaacg ccaagtagca
    12061 gcggatgatt tttatcccat gagacatcca gttcggcgaa gacatttttc gcctgcgcaa
    12121 gctggccttt gccgccgtcg ataaggatca catccgggat cttactgtcg tcaatggctt
    12181 taccataacg ccgacgcagc acctgattca tcgccgcata atcatcgccc ggcgtgatgc
    12241 ctgtaatgtt atagcgccga tactccgcac gcagcgggcc gttagcatca aacaccacac
    12301 aggaagcgac ggtttgttcg cccatggtat ggctgatgtc aaagcactcc atccgcttca
    12361 cttccggcaa tttcaacacg ctggcaagcg cggtcagtcg ctggtgaacg gtagattgct
    12421 gcgaaagttt gctggttaag gccgtcgccg cattggtgcg cgcgagtttc agataacgcg
    12481 ccctatcgcc gcgaggtttg gtttgaacat taatcttgcg tcccgccagt tctgaaaggg
    12541 aatcggcgag cagcgtttta tcgctaagat taaaatcgag caggatctca cccggtaagg
    12601 tgcgcatctg gctgccttgt aaatagaact gacctacgaa ggtttctacc acctcgctca
    12661 gttccgtacc gccaggcact ttcgggaaat agctgcggct gccgagcact ttgccctgac
    12721 gaatgaacaa tacgtggaca caagccatgc ccgcatcgaa cgccacacca ataacgtcaa
    12781 ggtcgtcgcc ggtattggaa acaaactgtt tttcggtgac gcgtcgcacc gcctgaattt
    12841 ggtcgcgaat acatgccgct tcttcaaact ccagattctg gctggcagtt tccatacggc
    12901 taatgagttg cgtaagcacc tgatcatctt tgccagacaa aaacaggcgc acatactcga
    12961 cctgctgagc gtattcttct tcactcacca gtccttcaac gcacggtccc agacagcgcc
    13021 ctatctggta ttgcagacac ggacgcgagc gattgcgata aacactattt tcgcactggc
    13081 gaatggggaa aatcttttgc agtagcgcca gtgtttcacg tacggcatag ccattcggga
    13141 acgggccgaa atattcacct ttggcatgct ttgcaccacg atgcatcgcc agacgcgggt
    13201 gggtatcgcc actcaggaag ataaaaggat aagatttatc atcgcgtagc aaaacgttgt
    13261 aacgcggctg atagagtttg atgtagttgt gttccagcaa cagcgcttcg gtttctgtat
    13321 gcgtaaccgt tacatcaatt tgctggatct gggcgaccag cgcttcggtt ttgcgcgaag
    13381 cgaggttgct gcggaaatag ctggaaagcc gttttttcag gtctttcgct ttgccgacat
    13441 agataaccgt accaccagca tcgtacatgc gataaacgcc tggctggctg gttacggttt
    13501 ttaaaaacgc ttttgcgtca aactgatcac tcactgactt gataatgtct ccgcattaca
    13561 cagaccatgg cgaattgcca ggtgagtcag ctcaacatcg ccatgaatgt ttagtttact
    13621 gaacatacga tagcggtagc tgttcaccgt tttcggactg agattgagct gttctgagat
    13681 ctcattgacc ttctggccct tagtgatcat cagcataatc tgcaattcac gttcagacaa
    13741 actggcaaat gggctttctg ttttttctgg ttcgatctgg cttaacgcca tttgttgagc
    13801 gatatcagaa gcaatgtaac gctgccctga atagacagaa cgaatcgcac tcacgacttc
    13861 ctgcggagcc gcgcctttgc tgaggtagcc agcagcacct gcctgcatga ctttcgctgg
    13921 taaagggttt tctgtatgga ccgtaagcat gatgatttta acatcagctg tggaacgcgc
    13981 gattttacgc gtcgcctcaa gaccgccaat gcccggcata ctcatgtcca ttagcaccac
    14041 gtcaacggca tttgtccggc accacttaac ggcgtcttca ccgcacgatg cctctccgac
    14101 gacttttata cccttaatat cttccagaat gcgtcgtatc cctgcgcgca ccagttcgtg
    14161 gtcatcaaca agtagaacgt tgatcaaagg aatatctcca gaaataggga taacgctact
    14221 gatagttagt cattcgtata ttagcggttt ttattgcaac tttgaaacgt taaaaatggt
    14281 gggtattcga tttttctctc gtttttgtca attcgattgt ccacagttat ggtcacgccc
    14341 gtcaccaacg gcttaccgga tcgtttcaca gtaagcccta cgatgaaatg tgacaaaaat
    14401 tgactttatt cagcaaaaat gaaaatcagc ccgctgaata tgtaacatta attaacccga
    14461 acaatacggg caaaaaacat tcatgataaa aatatttatt ggtcattata ttaacgtatt
    14521 ttatagcact gccgatatca cgctcaaaaa acaaccactg ctatttttag caaagcttat
    14581 ggtatactcc gccgccttaa catttttcac cacaaatttt cattgcaaca tgacgaggaa
    14641 aataaatgag tacgcctgat ttttctactg ccgagaataa tcaagaactg gcaaatgaag
    14701 tctcctgcct gaaagcgatg ctgacgctga tgctgcaggc gatgggacaa gctgacgcgg
    14761 gccgcgtgat gttaaagatg gaaaaacagc ttgcgctgat cgaagacgaa acccaggctg
    14821 cagtattttc caaaacggtt aagcaaatta aacaagccta ccgtcagtaa tataaaaccg
    14881 gctgagagcg tgcctttcag ccggttattg acaaaatcat cgctatctct gctgatgtct
    14941 tcccttccgc cgtccatttc agaatttctt tttcgcgctt gctgaaattc atctctggcg
    15001 tcatcactat ttcatcattt aaacgcatca gagccatcag actttcgcgc accagtaact
    15061 gcatttttaa ttgcagttca tcactaagaa tgggtatttc gcgcgtgctg caacgggaaa
    15121 aggacaaaaa gcccagcgcc cgatttggca gcattaaata ctgagtgaca ccgcggcgta
    15181 aaccatgtgc gtgcgcggct tcccataatg gctgtgcttc gctgaataag tcatcattcc
    15241 acattaaatg gccctgacta aagttttcag ggttcagcac aggatcaatt gcgagaaaat
    15301 tttttgcctg ataataacta acccacgact cagggtaatt ggtgtagaaa gccactttag
    15361 gtcgcgtgaa tggtaccggg tggcgaacac ataacgaata gtaatcgtac tccagctgct
    15421 gagcctgaag ctctatttca tggtaggcct cttctgcggc ctccatcctc tgaaaacgca
    15481 acagcatcgt gcgacgccag ctgaaaaaat ccgtatcctg catagtaaac cgcaacgccc
    15541 ctgagagtga gtatcattta taatgataat tcaaactatc acataaataa gatttatata
    15601 taatttatat tattcaggca atgaattact tttgcaagcc atcgcattct cttatgttat
    15661 taatgagtta tgctgatttg ttaagcagtt ttatcacgct tgaaatggcg tcccgccccg
    15721 acaggtgaat cgtcggggct gattttttct tattattgca gcagaagctt ctcgaggaac
    15781 tggcgggtgc gaggctgctg gggatcggca aataacgctt ttgctgcccc ctgctcgact
    15841 atccgcccct ggtccataaa gatcgcccgg tccgcaacat cccgggcaaa gctcatttcg
    15901 tgtgtcacaa tcaccatcgt gcgcttttcc tgcgccagct ggcggatggt gttcaggact
    15961 tcgcccacca gctctggatc cagcgccgac gttggctcgt caaacaaaat cacctcagga
    16021 cgcattgcca gtgcacgcgc aatcgcaaca cgctgctgtt gaccgccaga caaacgacgt
    16081 ggatagctgg tttctttacc ggccagccca actttcgcca gcagctcgcg agcgcgcgcc
    16141 gtggcctctt ctttcggttc gcctttgacg atcaccggcc cttcaataat gttttccagc
    16201 accgtacgat gcggaaacaa attaaagttc tggaagacaa acccgacgtg ctgacgcaac
    16261 tggcgaatca gcgatttttg ctgacttaat gaacgtgcag tatcaatagt gatatcgcca
    16321 acggtgatcg ttcccgcttc cggttgttcc agcagattta tgctacgtag caatgtggtt
    16381 ttgccggaac cactcggacc gataattgcc accacttcgc caggctttac ctcgaggtcg
    16441 ataccgtgca gcaccgtctg accgtggaat tttttcacca ggttcttaac ttcaatggca
    16501 ctcattttgg ttctctctcc tggcgattaa gttgattctc aaaatggttc tgcaacgtcg
    16561 ataacacagt cgccatgatc cagtagatga gcgaagccgc caggtacatg gtgaacacct
    16621 ccagcgtgcg cgaggtaatc aactgcgcct gacggaacag ctccggcacc tggattgtcg
    16681 cggccagcga cgtatctttt accagactga taaagctgtt cgacagcggc ggcaacgcca
    16741 cccgcgccgc ctgtggcaaa atcgcgcgac gcatggtctg ccacggcgtc ataccaatac
    16801 tggcagcggc ttcccactga cctttatcaa tagaagaaat ggcggcgcgc agcgtttcgg
    16861 cggcataggc ggcagtgttc agcgacagac caatcattgc tgacggaatg ggatctaatt
    16921 cgataccaaa ctgcggcagg ccgtaataga tcataaacag ttgtgcgatg agcggcgtac
    16981 cacgaaaaat agagatataa aaacgcgcca gccagcgcac gggccagatt ggcgacaggc
    17041 gcatcagcgc gagaataaac ccgagtagta agccaaaaaa catgccgccg atactgagtt
    17101 gcagggtata cccagcccct ttcaacagga acggcaagga atcaataacc agttgtatac
    17161 tttcttgcat gagcgttccc ggagctaaac gtggggatga taggcgaaca gcgcaggcgc
    17221 gccgccggta tgaataaaca gaatcggccc ttcatctttg aagcgtttct gactgatacc
    17281 gtcaatcagc cccgccatcg cttttccggt atacacagga tcaagcagaa tgccttccag
    17341 acgcgccagc aatttcactg cttccatgcc ttcgtcgttc ggcacgccgt agccaggtgc
    17401 aaaatagtca tcccagagta aaatttccgc tgatgcggtc agctccagtt ctttcgcaat
    17461 cgcctgttgt aggttaacca ctttcggcaa ttgatcggca acggaacgcg acacggtcac
    17521 gccaatcagt tcgctttcag gcatcaggtg ttccagccca acagccagtc cggcgtgagt
    17581 tccggcactg cccgatgcga ctaccaccga cgaaatatta accgcccctt cacactgttg
    17641 cgcgatttcc agcgcactct ccacataacc tagcgcgcca agagcattag aaccgccaac
    17701 cggaatgaca tacgggcgaa agccttgtgc ttcgactcgc gtcgccagtt cttccagttg
    17761 ggcattggga tcggtcagtg cgtcgcacat ttcaatctgg gtattgaaca gatccagcaa
    17821 caaacgattg ccgttggtta aatagttttc tgcggttgtg ccaataggat tttccagcag
    17881 cgccacgcag tgcagaccga gtttcgccgc gactgcggca gtctggcgca catggttaga
    17941 ctggatcgcg ccggcagtaa tcagcgtatc ggcaccttcg cgcagggcat ctgccgcgag
    18001 aaattccagc ttacgtaatt tattgccgcc cattgccatg ggggtgacct catcccgttt
    18061 gatgaaaatt tcccgtccaa gataatcaga aaagcgcggc agatattcga gcggcgttgg
    18121 cgcgccgata aactccaacc gtggaaaacg ggttaaatta tgcagtggca taacagcctc
    18181 cgatgtgtgt tgttgtgatt ttcttattat gcacgctgaa aacgcgtaaa taaaaaaggc
    18241 gctagtgaaa gcgccctttt ttgtcattat gctgattatt tggtcacatc agcaccaaac
    18301 catttttcgg aaagagcttg cagagtgccg tctttttgca tttccgcaat tgcatcattc
    18361 accgctttca gcaggtcttc atttccttta cgcagcgcca cgccagactc ctgacgggag
    18421 aatgcttcac cggttactgc cagcgtatcg ttggttttct tcaccagatc cagcgccgcc
    18481 agacgatcaa cgaggatcgc atcgatacgc cctacgcgca gatcctgata tttggtcggg
    18541 tcatcatcat aggtacgcac atcgacaccc tgaacattct gccgcagcca ctcttcatag
    18601 ttggtgccca gaccgacacc cacttttttg cctttcagat cagcagccgt tttaatggtg
    18661 ccttcgttac cttttttcac cagcgcctga ataccggaaa tggtgtaagg ggtagagaaa
    18721 tcgtattttt tcttgcgctc gtcagaaatg gtgacctgat taatcaccac atcaatgcgt
    18781 ttagagtcca gcgacgccag cataccgtcc catttggtcg gttttagtga cgcctcaacg
    18841 ccaagatgtt ttgccagctg ttgggcaaat tccacttcaa aaccggttaa tttgccgtca
    18901 tccccctgaa aactgaacgg cggataagtg ccttccagcc ctaccagcag cgtgccgctc
    18961 tctttgactt tattaagcag accttcatct gcaaaacttt taacgctcat gcccgcaacc
    19021 agcgccacgg ccatcacacc catcaatgcc tgacgtccca gatgtgctaa tttcatattc
    19081 accccgaatg ttgttatgtc tgtttgcagt gtagagccat tgtttgtaaa cacaaaaaca
    19141 actccgctac atcttattct tatttaatat atatcagaag caggcaggct ggaggatttc
    19201 tgcacaagtc cggtacaggt ttggcgctga tagtgttgat acttccgtaa tgcgatgcgg
    19261 taattgttgg tgctggtggt gggcagccac ggggcgagga tttcatcaag gaaaccgtcc
    19321 tgcagctgat cgagggaaat attttgctca tgtagatgat tacccaaacg gcgcagacga
    19381 acgacatatt cacgcaccgt acctgggctc atttcagttt gctcaaaaag aaatttctta
    19441 aagccgataa tatcgaaaaa atcactctgc gttttgcaat gtaaatcacc gcaaaatcgg
    19501 cacaatgccg cccatgattg ttgttccgtt tgccagcctt tttcatcaag cagcgtgtcc
    19561 aggcgggaaa tctcgatttt attcactatt ttgccgtcgc gaactaaggt aatgcgatcg
    19621 agtaatttac ggcaatgcgc gcaatgggtc tggctgtgtt taaagtcttt aagatagcgg
    19681 cttaatggcc gtcttttcag gtgctgcacc atcattaaga actcctggta gtcaaagtta
    19741 aagtgcggca tttactgacg ttataactta cccagtttag tgcgtaaccg tttaatgtcc
    19801 tggctgtgta actgactgac ccgcgattcc ccgacctcca gcaccgcgcc aatctctttg
    19861 agattcagct cttcctggta atagagggtt aataccagtt tttcgcgctc cggcaacgtt
    19921 tcgatggctt ccatcacccg ctggcgcaga ttactgtcca gcagttgttg tagcgggttt
    19981 tctcgctgat gatcatcagt aaccagttcg atgctatcgc cgtgctcttc acgccactca
    20041 tcgtaggaga agagctggct gttattggtg tcgagcagca tttgacgata atcggcaata
    20101 tcgatcccta aacgttccgc tacctcagtt tccgtggcgt tgcggccaag ttcctgctcc
    20161 agttgcccta ttgcctgtgc cacttcacgc gcgttgcgtc gcacgctgcg cggcacccag
    20221 tcacggctgc gaagttcatc cagcatagcg ccacggatac gctacactgc gtaagttgta
    20281 aatgccgttc cttgtagggc gtcatagcgt tcgacagcat taagtaaccc aatgccgccc
    20341 gcctgtagca gatcgtcaag ttccacgctc gcgggcagtc gaacctgcag gcgcaatgct
    20401 tcgtgacgca ccagcgggac ataacgctgc cacagcgagt gtttatccat tacaccttca
    20461 gcggtataga gtgaattcac gataaacagc cctgcgttat atgagttatc ggcatgatta
    20521 tccgtttctg cagggttttt aatcggacga ttagtgggtg aaatgagggg ttatttgggg
    20581 gttacgcaaa aagccgaatg taaaaaaccc cgccggtggc ggggtttgag aggcattcag
    20641 acgattaacc ctgcagcaga gacagaacct gctgcggtac ctggttggct tttgccagca
    20701 cggagttacc ggcctgctgg ataatctgcg ctttcgacat gttggacact tcggtcgcat
    20761 agtcggcgtc ctgaatacgg gactgcgctt cagacaggtt ggtagtggtg ttgttcaggt
    20821 tggtgactgc ggaatccaaa cggttttgca ccgcacccag ggaggagcgg aatttgtcga
    20881 tggagctgat ggcttcatcc agagctttca ggggatcggc tgtcgtagct gcttttgttt
    20941 cagcatctgt cgtcaattta ccatcagcat ctttataaac tgccttaccg ctgccgttag
    21001 tcactgaacc atcatcctgt aggtaaaaat ctacagtatc agatgtggtc aacgcctcgt
    21061 tgttatcaac ataaaccgaa tcaacacctt ttgttacagc gccagttgtg ccgttgacgc
    21121 tataacctgc agtagtgaaa tcaacattgt ttcccgaggt tgatacagct ttatcgaaag
    21181 ctgcctgatc aacttttgca cctgttactg aataagtaat agtgtccggc gtagtactat
    21241 taccggtaaa ggagatagag gtaccgccaa tgtcaatggt tgtcgctttg gtattattgg
    21301 ctgcaagtgt ggacagacta gcctcagtca aagaagcacc agagccgttt ttcgttaagc
    21361 gcccagttgt atcaatgtag agtttatctc cattgcttgc cgtaatttta ccatcactgc
    21421 tgatctgaac atcctgtgca gaaccatcga tagtaatagt gcccttagcg gtatcaccaa
    21481 ctcccggggt caaatatttc tgaacatcgg cagctgaagc cgttgtggta tcaaaagagt
    21541 atgacttact tgcactgtca tacttgtaat tcgttgcact agccgccgtt ccgaagccgt
    21601 tattaatggt tgcctgaaca gtatcaccat cactaactcc agccagcaaa tcagacgctt
    21661 tagcagcatc gtaacccgca ctcacagtat atttgttgcc gaccactgtt gcattagcag
    21721 ctaccaagtc agctttagat gcagcagtat tagccacagc cccgccacca ttcacattaa
    21781 acccattcag ccccagcgta tcagagtcaa ttttcttcag atcaatagtg atagtctggc
    21841 cgtcattcgc accaacctga attttcatcg aaccgtcttt tgccagtacg ttcacgccgt
    21901 tgaactgggt ctgaccggat acgcggtcaa tttcgtcgag acgggatttg atttcgtcct
    21961 gaatggagtc cagatccgaa tcagagttag tcccggtaga agcctgaacc gtcagctcac
    22021 ggatacgctg taagttgttg ttgatttcgg acagcgcgcc ttcagtggtc tgtgcaacag
    22081 aaataccgtc gttggcgtta cgtgcagcct gagtcaggcc tttaatgtta gaagtaaaac
    22141 ggttagcaat cgcctgaccc gcggcgtcat ccttcgcgct gttaatacgc aagccagaag
    22201 acagacgctc gatagaactc gacagcgcag actggttctt gttgatatta ttttgagtga
    22261 tcagcgagag gctgttggta ttaatgactt gtgccatgat tcgttatcct atattgcaag
    22321 tcgttgatta cgttttgggt ttccacccgt cggctcaatc gccgtcaacc ctgttatcgt
    22381 ctgtcgtaaa acaaccttta gaattttttt cacaaacagc cattttttgt tagtcgacga
    22441 aatactcttt tctctgcccc ttattcccgc tattaaaaaa aacaattaaa cgtaaacttt
    22501 gcgcaattca ggccgataac cccggtattc gttttacgtg tcgaaagata aaaggaaatc
    22561 gcatggcaag tatttcatcg ctgggagtcg ggtcaggtct ggatttaagt tcaatccttg
    22621 atagcctcac cgccgcgcaa aaagcgacgc taacccctat ttcaaatcag caatcgtcgt
    22681 ttaccgctaa acttagcgcc tacggtacgc tgaaaagcgc gctgacgact ttccagaccg
    22741 ccaatactgc attgtctaaa gccgatcttt tttccgccac cagcaccacc agcagcacca
    22801 ccgcgttcag tgccaccacc gcgggtaacg ccatcgccgg gaaatacacc atcagcgtca
    22861 cccatctggc gcaggcgcaa accctgacca cgcgcaccac cagagatgat acgaaaacgg
    22921 cgatcgccac cagcgacagc aaactcacca ttcaacaagg cgacgacaaa gatccgatta
    22981 ccattgatat cagcgcggct aactcatcgt taagcgggat ccgtgatgcc atcaacaacg
    23041 caaaagcggg cgtaagtgcg agcatcatta acgtgggtaa cggtgaatat cgtctgtcag
    23101 tcacatcaaa tgacaccggc cttgataatg cgatgacact ctcggtcagc ggtgatgatg
    23161 cgctacaaag ttttatgggc tatgacgcca gtgccagcag caacggtatg gaggtctcgg
    23221 tcgccgccca gaatgcgcag ctgacggtca acaacgtcgc catcgagaac agcagcaaca
    23281 ctatcagcga cgcgctggaa aacatcactc tgaacctgaa cgatgtcacc acgggcaacc
    23341 agacgctaac catcactcag gacacctcca aagtgcaaac ggcgattaaa gactgggtga
    23401 atgcctataa ctcgctaata gataccttca gcagcctgac caaatacacc gccgtagatg
    23461 cgggagctga tagccagagt tctagcaatg gcgcactgct cggcgactcc acgctgcgga
    23521 cgattcagac gcagttgaag tcgatgctga gtaataccgt cagttcttcc agctataaaa
    23581 cgctggcgca gattggtatc acgaccgatc ccagcgatgg caaactggaa ctggatgccg
    23641 acaaactcac cgctgcactg aaaaaagatg ccagcggcgt aggtgcattg attgttggcg
    23701 atggtaaaaa aaccggcatc acaaccacca tcggcagcaa cctgaccagt tggctttcga
    23761 caacgggcat tattaaagcc gctaccgatg gcgttagtaa aaccctgaat aagttaacta
    23821 aagactacaa cgccgccagc gatcgcattg atgcgcaggt cgcgcgctac aaagaacaat
    23881 ttacccaact ggacgtttta atgacctcgt taaacagcac cagcagctac ttaacgcagc
    23941 agttcgaaaa caacagtaat tccaagtaat cattattcat cgggagacag gtcatgtacg
    24001 cggcaaaagg cacccaggcc tatgcacaaa ttggcgtcga aagcaccgta atgagcgcca
    24061 gccaacagca gttggtcacc atgctatttg atggagtgct gagcgcactg gttagagcgc
    24121 gactgtttat gcaggacaac aatcagcaag gcaaaggcgt ctctttgtca aaagcgatca
    24181 acatcattga gaacggactg cgggtgagtc ttgatgaaga gagcaaagac gaattaaccc
    24241 aaaacttaat tgctctttat agctatatgg tcaggcgttt gctgcaagcc aatttacgca
    24301 acgatgtctc agcagtcgaa gaggtggaag cattaatgcg taatattgcc gatgcctgga
    24361 aagagtcttt actctcccct tcttcgattc aggacccagt ctgatgaaca atgcaccgca
    24421 tttgtatttc gcctggcaac aactcgtcga aaaaagccag ctcatgttac gcctggcaac
    24481 ggaagaacaa tgggacgaac tcatcgctag cgaaatggcg tatgtgaatg cggtgcagga
    24541 gatcgcacac ctgactgaag aggttgcccc gtccactacg atgcaggagc agctccgccc
    24601 gatgctgcac ctgattctcg acaacgaaag caaggtaaag cagttattac agattcggat
    24661 ggatgaactg gcgaaactgg tcggtcagtc atcggtgcaa aaatcggtgt taagtgccta
    24721 tggcgatcag ggcggctttg tgctggctcc gcaggataac ctcttttgaa tcttaatgag
    24781 ccgatggctc gcgaataatc cgattacggc tacgcttcta atattcccct tgaatggagt
    24841 cgaagaatgc gtaatcccac gctgttacaa tgttttcact ggtattaccc ggaaggcggt
    24901 aagctctggc ctgaactggc cgagcgcgca gacggtttta atgatattgg tatcaatatg
    24961 gtctggttgc cgcccgccta taaaggcgca tcgggcggct attcggtcgg ctacgactcc
    25021 tatgatttat ttgatctagg tgagtttgat cagaaaggca gcatccctac taaatatggt
    25081 gataaagcac aactgctggc cgccattgac gccctgaaac gtaatgacat tgcggtgctg
    25141 ctggacgtgg tagtcaacca caaaatgggc gcggatgaaa aagaagctat tcgcgtgcag
    25201 cgtgtaaatg ctgatgaccg tacgcaaatt gacgaagaaa tcattgagtg tgaaggctgg
    25261 acgcgttaca ccttccccgc tcgtgccggg caatactcgc agtttatctg ggatttcaaa
    25321 tgttttagcg gtatcgacca tatcgaacac cctgatgaag atggcatttt taaaattgtt
    25381 aacgactata ccggagaagg ctggaacgat caggttgatg atgaactcgg taatttcgat
    25441 tatctgatgg gtgagaatat cgattttcgc aatcatgccg tgacggaaga gattaaatac
    25501 tgggcgcgct gggtgatgga acaaacgcaa tgcgacggct ttcgtcttga tgcggtcaaa
    25561 catattccgg catggtttta taaagagtgg atcgaacacg tacaggaagt tgcgccaaag
    25621 ccgctgttta ttgtggctga atgctggtcg catgaagtcg ataaactgca aacgtatatt
    25681 gatcaggtgg aaggcaaaac catgctgttt gatgcgccgc tgcagatgaa attccatgaa
    25741 gcatcgcgca tgggtcgcga ctacgacatg acgcagatct tcactgggac gctggtggaa
    25801 gccgatcctt ttcacgccgt aaccctcgtt gccaaccacg acacccaacc gttgcaagcc
    25861 ctcgaagcac cggtcgaacc gtggtttaaa ccgctggcgt atgccttaat tttgttgcgg
    25921 gaaaatggcg ttccttcggt gttctatccg gacctctacg gtgcacatta cgaagatgtc
    25981 ggtggtgacg ggcaaaccta tccgatagat atgccaataa tcgaacagct tgatgagtta
    26041 attctcgccc gtcagcgttt cgcccacggt gtacagacat tatttttcga ccatccgaac
    26101 tgcattgcct ttagccgcag tggcaccgac gaatatcccg gctgcgtggt ggtcatgtcg
    26161 aacggagatg atggcgaaaa aaccattcat ctgggagaga attacggcaa taaaacctgg
    26221 cgtgattttc tggggaaccg gcaagagagc gtagtgacag acgaaaacgg cgaagcgacc
    26281 ttcttttgca acggcggcag cgtcagcgtg tgggttatcg aagaggtgat ttaaatccat
    26341 ccccggcggc aagccgggga gatttcatta cggcagtggc gtcggtaacg gcgctttatc
    26401 cagcgcggcg gcacactctg ccgtcgggcg atccacacgc tccatcgtca taccatcgta
    26461 ctcaatggtg ttaccgtcac gctcaacctc gtacagctca cgttttacgg taacgttagt
    26521 gagatcatca gacatcaatg tcaacttacc cggcacggca atcccccgct gccactgacg
    26581 gcaatccaag gtatcgccct ctttagtgac aatcaggctg cctatcgctt ccggactgac
    26641 cagcgcacgt tgcggccctt tggtctgcca gtagcccgcc agccagtccg gcgcgggggt
    26701 tttcacgaca ttgttatagt tttcgacttc ggcacagccc gccagcaaca tgagcgcgcc
    26761 tgcaattgcg agttttttca tcattcgtcc tgcatgagaa gaaaagagga ttgtggcatt
    26821 aaagctctga tgtcgccagc ctttccaggt tcagagagaa atttgatcta actattattt
    26881 ttgtgaatta ttaccccgaa aataaatttg catggtgtat gattcgcggc tcttgtttca
    26941 aaccttctga gttcagaggc tacattcatg tcatggcagc aattcaaaca cgcctggttg
    27001 attaaattct gggcacccat ccctgcggtc atcgcggcgg gtattctctc cacttactat
    27061 tttggcatta ctggcacctt ttgggctgtc acgggtgaat ttacccgttg gggcggtcag
    27121 ctcctgcagc tgtttggcgt ccatgctgaa gagtggggtt attttaaaat tatccatctg
    27181 gaaggatcgc cattaacccg gatcgacggc atgatgatcc tcggtatgtt tggcggctgc
    27241 tttgctgctg cgctgtgggc caacaatgtc aaactacgca tgccacgcag ccgtatccgc
    27301 attatgcagg ccatcattgg cggcattatc gccggttttg gcgcgcgtct ggcaatgggc
    27361 tgtaacctgg cggcgttctt taccggcatt cctcagttct cgctgcatgc ctggttcttt
    27421 gccatcgcca ctgccattgg ttcatggttt ggcgcgcgct ttaccctgct gcccatcttc
    27481 cgtattcccg tgaaaatgca gaaagtttct gccgcctcac cgctgacgca aaaaccggat
    27541 caggcgcggc gtcgttttcg tctcgggatg ctggtctttt tcggcatgct gggctgggcg
    27601 ctgctcacgg cgatgaacca accaaaactg gggctggcaa tgctgtttgg cgtcggcttt
    27661 ggtttactga ttgaacgtgc gcaaatctgc tttacttcgg cgttccgcga tatgtggatc
    27721 accggacgta cccatatggc gaaagcaatc attatcggta tggcggtgag tgccatcggg
    27781 atcttcagtt acgtacagtt aggcgttgaa cctaaaatca tgtgggcggg accaaatgcg
    27841 gtaattggtg gtttactttt tggttttggc atcgtgctgg caggcggctg cgaaaccggc
    27901 tggatgtacc gcgcggtaga aggccaggtg cactactggt gggtcggtct gggcaacgtg
    27961 atcggctcaa cgattctggc gtattactgg gatgatttcg ctccggcgct ggccaccgac
    28021 tgggacaaaa tcaacctgct gaaaactttt ggtccgatgg gaggcctgct ggtgacatat
    28081 ttgctgctgt ttgctgcgct gatgttgatt atcggctggg aaaaacgctt cttccgccgt
    28141 gaggcaccgc agactgctaa ggagatcgca tgaaaaatat cgttcctgat taccgccttg
    28201 atatggtggg tgaaccctgc ccttatccgg cagtcgcaac ccttgaggcg atgccgcaat
    28261 taaaaaaagg tgagatcctg gaagtggtga gcgactgtcc gcagtcgatc aataatattc
    28321 cactggatgc gcgtaatcac ggctacacgg tgctggatat tcagcaagac gggccgacca
    28381 ttcgttattt aattcagaag taatccctca ttcccgctgt ggcccacagc gggaacatcg
    28441 ttacctctga cacatagatt ttacccgccc cctctcctat tcttttcagt atgatctcag
    28501 gaggttgcta tgtgtggacg ctttgcccaa tcccaaacgc gtgaggatta ccttgcgctt
    28561 ctcgcggaag atattgaacg cgatattccc tacgatcccg aacccattgg cagatacaat
    28621 gtcgcgccgg gaaccaaagt tcttctgctc agtgaacgtg atgaacacct tcatctggat
    28681 ccggttttct ggggatatgc tcccggatgg tgggataaac cgccgctgat taacgcccgc
    28741 gtagaaactg cctcaaccag tcgcatgttt aaaccgctct ggcaacatgg tcgggcaatc
    28801 tgttttgccg atggctggtt tgagtggaaa aaagaaggcg acaaaaaaag ccttatttta
    28861 tctatcgcgc tgacggacaa cctgttttta tggccgcgat aggtagcata ccgtttgagc
    28921 gtggtgacga agccgaagga tttttgatcg tcactgcggc ggcagatcaa ggtctggtag
    28981 atattcatga ccgccgcccg ctggttctgt cgccagaagc tgcacgggaa tggatgcggc
    29041 aagacattgg tggtaaagaa gcctcagaaa tcgctaccag aagctgtgtg ccagcaaacc
    29101 agttcacctg gcaccctgtg tcgcgcacgg tgggtaatgt caaaaaccag ggcgcggagt
    29161 taattcaacc tgtttgttga catcctccac gccctgaatg acgaggaccc ctgctacgtt
    29221 caggctgtcg cctgaatcat ttcggtgggt tcctgcttca acgagcggcc tgactgcacc
    29281 atctctccac aggcaagaac ggcgtgcccc gccgctaaaa tgttacgagc tccatttaca
    29341 tcggcgttcg ctgtatatcc acatacctgg catctgaatt tactttgtga caggcgattt
    29401 tctttcgctg tatgaccaca gcacgcgcaa cgctggcttg tgtacgctgg cggaacagca
    29461 agcacctgac cgccacgcca gagctgcttg tactcaagct ggcggcgcat ttcataccag
    29521 ccctgatcca gtatcgaacg gtttaaacct gattttgccc ggacattgcg ccccggctgg
    29581 ctgaccgtac ccgctgctga ctttgacatg ttgctgacct tcaaatcctc aatgactatc
    29641 attacgtggt ttttgctg
//
LOCUS       DAJTRC010000046        29456 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.046, whole genome shotgun sequence.
ACCESSION   DAJTRC010000046 DAJTRC010000000
VERSION     DAJTRC010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.046"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..203)
                     /locus_tag="OO357_002166"
     CDS             complement(<1..203)
                     /locus_tag="OO357_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HCR5887105.1"
                     /translation="MLELNAKTTALVVIDLQEGILPFAGGPHTADEVVNRAGKLAAKF
                     RASGQPVFLVRVGWSADYAEALK"
     gene            513..2285
                     /gene="aspS"
                     /locus_tag="OO357_002167"
     CDS             513..2285
                     /gene="aspS"
                     /locus_tag="OO357_002167"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HCR5887106.1"
                     /translation="MRTEYCGQLRLSHVGQQVTLCGWVNRRRDLGSLIFIDMRDREGI
                     VQVFFDPDRADALKLASELRNEFCIQVTGTVRARDEKNINRDMATGEIEVLASSLTII
                     NRADVLPLDSNHVNTEEARLKYRYLDLRRPEMAQRLKTRAKITSLVRRFMDDHGFLDI
                     ETPMLTKATPEGARDYLVPSRVHKGKFYALPQSPQLFKQLLMMSGFDRYYQIVKCFRD
                     EDLRADRQPEFTQIDVETSFMTAPQVREVMEALVRHLWLEVKGVDLGDFPVMTFAEAE
                     RRYGSDKPDLRNPMELTDVADLLKSVEFAVFAGPANDPKGRVAALRVPGGASLTRKQI
                     DEYGNFVKIYGAKGLAYIKVNERAKGLEGINSPVAKFLNAEIIEAILDRTAAQDGDMI
                     FFGADNKKIVADGMGALLLKVGKDLGLTDESKWAPLWVIDFPMFEDDGEGGLTAMHHP
                     FTSPKDMTAAELKAAPENAVANAYDMVINGYEVGGGSVRIHNGDMQQTVFGILGINEE
                     EQREKFGFLLDALKYGTPPHAGLAFGLDRLTMLLTGTDNIRDVIAFPKTTAAACLMTE
                     APSFANPTALAELSIQVVKKAENN"
     gene            2403..2855
                     /gene="nudB"
                     /locus_tag="OO357_002168"
     CDS             2403..2855
                     /gene="nudB"
                     /locus_tag="OO357_002168"
                     /EC_number="3.6.1.67"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin triphosphate diphosphatase"
                     /protein_id="HCR5887107.1"
                     /translation="MKDKVYKRPVSILVVIYAQDTKRVLMLQRRDDPDFWQSVTGSVE
                     EGETAPQAAMREVKEEVTIDVVAEQLTLIDCQRTVEFEIFSHLRHRYAPGVTRNTESW
                     FCLALPHERQIVFTEHLAYKWLDAPAAAALTKSWSNRQAIEQFVINAA"
     gene            2884..3624
                     /locus_tag="OO357_002169"
     CDS             2884..3624
                     /locus_tag="OO357_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000907250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5887108.1"
                     /translation="MAGHSKWANTRHRKAAQDAKRGKIFTKIIRELVTAAKLGGGDPD
                     ANPRLRAAVDKALSNNMTRDTLNRAIARGVGGDDDANMETIIYEGYGPGGTAIMIECL
                     SDNRNRTVAEVRHAFSKCGGNLGTDGSVAYLFSKKGVISFEKGDEDTIMEAALEAGAE
                     DVVTYDDGAIDVYTAWEEMGKVRDALEAAGLKADSAEVSMIPSTKADMDAETAPKLMR
                     LIDMLEDCDDVQEVYHNGEISDEVAATL"
     gene            3659..4180
                     /gene="ruvC"
                     /locus_tag="OO357_002170"
     CDS             3659..4180
                     /gene="ruvC"
                     /locus_tag="OO357_002170"
                     /EC_number="3.1.21.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005068993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HCR5887109.1"
                     /translation="MAIILGIDPGSRVTGYGVIRQVGRQLSYLGSGCIRTKVDDLPSR
                     LKLIYAGVTEIITQFQPDYFAIEQVFMAKNADSALKLGQARGVAIVAAVNQELPVFEY
                     AARQVKQTVVGMGSAEKSQVQHMVRTLLKLPANPQADAADALAIAITHCHVSQNAMQM
                     SESRLNLTRGRLR"
     gene            complement(4182..4784)
                     /locus_tag="OO357_002171"
     CDS             complement(4182..4784)
                     /locus_tag="OO357_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebB family permuted papain-like enzyme"
                     /protein_id="HCR5887110.1"
                     /translation="MNINYPAEYEIGDIVFTCIGAALFGQISAASNCWSNHVGIIIGH
                     NGEDFLVAESRVPLSTITTLSRFIKRSSNQRYAIKRLDAGLTEQQKQRIVEQVPSRLR
                     KLYHTGFKYESSRQFCSKFVFDIYKEALCIPVGEIETFGQLLNSNPNAKLTFWKFWFL
                     GSIPWERKTVTPASLWHHPGLVLIHAEGVETPQPELTEAV"
     gene            4854..4919
                     /gene="yobI"
                     /locus_tag="OO357_002172"
     CDS             4854..4919
                     /gene="yobI"
                     /locus_tag="OO357_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response small protein YobI"
                     /protein_id="HCR5887111.1"
                     /translation="MYIFITHFFTEYVIFKYLLPI"
     gene            5058..5669
                     /gene="ruvA"
                     /locus_tag="OO357_002173"
     CDS             5058..5669
                     /gene="ruvA"
                     /locus_tag="OO357_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012131997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HCR5887112.1"
                     /translation="MIGRLRGIIIEKQPPLVLIEVGGVGYEVHMPMTCFYELPEAGQE
                     AIVFTHFVVREDAQLLYGFNNKQERTLFKELIKTNGVGPKLALAILSGMSAQQFVNAV
                     EREEVGALVKLPGIGKKTAERLIVEMKDRFKGLHGDLFTPAADLVLTSPASPATDDAE
                     QEAVAALVALGYKPQEASRMVSKIARPDTSSETLIREALRAAL"
     gene            5678..6688
                     /gene="ruvB"
                     /locus_tag="OO357_002174"
     CDS             5678..6688
                     /gene="ruvB"
                     /locus_tag="OO357_002174"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HCR5887113.1"
                     /translation="MIEADRLISAGTTLPEDVADRAIRPKLLEEYVGQPQVRSQMEIF
                     IKAAKLRGDALDHLLIFGPPGLGKTTLANIVANEMGVNLRTTSGPVLEKAGDLAAMLT
                     NLEPHDVLFIDEIHRLSPVVEEVLYPAMEDYQLDIMIGEGPAARSIKIDLPPFTLIGA
                     TTRAGSLTSPLRDRFGIVQRLEFYQVPDLQYIVSRSARFMGLEMSDDGAQEVARRARG
                     TPRIANRLLRRVRDFAEVKHDGTISADIAAQALDMLNVDAEGFDYMDRKLLLAVIDKF
                     FGGPVGLDNLAAAIGEERETIEDVLEPYLIQQGFLQRTPRGRMATTRAWNHFGITPPE
                     MP"
     gene            complement(6835..7620)
                     /gene="znuB"
                     /locus_tag="OO357_002175"
     CDS             complement(6835..7620)
                     /gene="znuB"
                     /locus_tag="OO357_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease subunit ZnuB"
                     /protein_id="HCR5887114.1"
                     /translation="MIELLFPGWLAGIMLACAAGPLGSFVVWRRMSYFGDTLAHASLL
                     GVAFGLLLDVNPFYAVIAVTLLLAGGLVWLEKRPQLAIDTLLGIMAHSALSLGLVVVS
                     LMSNIRVDLMAYLFGDLLAVTPEDLISIAIGVVIVVAILFWQWRNLLSMTISPDLAFV
                     DGVKLQQVKLLLMLVTALTIGVAMKFVGALIITSLLIIPAATARRFARTPEQMAGVAV
                     LVGMVAVTGGLTFSAFYDTPAGPSVVLCAALLFIISMMKKQAS"
     gene            complement(7617..8372)
                     /gene="znuC"
                     /locus_tag="OO357_002176"
     CDS             complement(7617..8372)
                     /gene="znuC"
                     /locus_tag="OO357_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter ATP-binding protein ZnuC"
                     /protein_id="HCR5887115.1"
                     /translation="MTSLVSLENVSVSFGQRRVLSDVSLELKPGKILTLLGPNGAGKS
                     TLVRVVLGLVTPDEGVIKRNGKLRIGYVPQKLYLDTTLPLTVNRFLRLRPGTHKEDIL
                     PALKRVQAGHLINAPMQKLSGGETQRVLLARALLNCPQLLVLDEPTQGVDVNGQVALY
                     DLIDQLRRELDCGVLMVSHDLHLVMAKTDEVLCLNHHICCSGTPEVVSLHPEFISMFG
                     PRGAEQLGIYRHHHNHRHDLQGRIVLRRGNDRS"
     gene            8451..9383
                     /gene="znuA"
                     /locus_tag="OO357_002177"
     CDS             8451..9383
                     /gene="znuA"
                     /locus_tag="OO357_002177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     ZnuA"
                     /protein_id="HCR5887116.1"
                     /translation="MLHKKTLLFAALSAALWGGATQAADAAVVASLKPVGFIASAIAD
                     GVTETEVLLPDGASEHDYSLRPSDVKRLQNADLVVWVGPEMEAFMQKPVSKLPEAKQV
                     TIAQLEDVKPLLMKSIHGDDDDHDHAEKSDEDHHHGDFNMHLWLSPEIARATAVAIHG
                     KLVELMPQSRAKLDANLKDFEAQLASTETQVGNELAPLKGKGYFVFHDAYGYFEKQFG
                     LTPLGHFTVNPEIQPGAQRLHEIRTQLVEQKATCVFAEPQFRPAVVESVARGTSVRMG
                     TLDPLGTNIKLGKTSYSEFLSQLANQYASCLKGD"
     gene            9399..10721
                     /gene="mepM"
                     /locus_tag="OO357_002178"
     CDS             9399..10721
                     /gene="mepM"
                     /locus_tag="OO357_002178"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein DD-endopeptidase MepM"
                     /protein_id="HCR5887117.1"
                     /translation="MQQIARSVALAFNNLPRPHRVMLGSLTVLTLAVAVWRPYVYHRD
                     ATPIVKTIELEQNEIRSLLPEASEPIDQAAQEDEAIPQDELDDKIAGEAGVHEYVVST
                     GDTLSSILNQYGIDMGDITQLAAADKELRNLKIGQQLSWTLTADGELQRLTWEVSRRE
                     TRTYDRTAANGFKMTSEMQQGEWVNNLLKGTVGGSFVASARNAGLTSAEVSAVIKAMQ
                     WQMDFRKLKKGDEFAVLMSREMLDGKREQSQLLGVRLRSEGKDYYAIRAEDGKFYDRN
                     GTGLAKGFLRFPTAKQFRISSNFNPRRTNPVTGRVAPHRGVDFAMPQGTPVLSVGDGE
                     VVVAKRSGAAGYYVAIRHGRSYTTRYMHLRKILVKPGQKVKRGDRIALSGNTGRSTGP
                     HLHYEVWINQQAVNPLTAKLPRTEGLTGSDRREFLAQAKEIVPQLRFD"
     gene            10841..11812
                     /gene="lpxM"
                     /locus_tag="OO357_002179"
                     /gene_synonym="msbB"
     CDS             10841..11812
                     /gene="lpxM"
                     /locus_tag="OO357_002179"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707715.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5887118.1"
                     /translation="METKKNNSEYIPEFDKSFRHPRYWGAWLGVAAMAGIALTPPKFR
                     DPILARLGRFAGRLGKSSRRRALINLSLCFPERSEAEREAIVDEMFATAPQAMAMMAE
                     LAIRGPEKIQPRVGWQGLEIIEEMRRNNEKVIFLVPHGWAVDIPAMLMASQGQKMAAM
                     FHNQGNPVFDYVWNTVRRRFGGRLHARNDGIKPFIQSVRQGYWGYYLPDQDHGPEHSE
                     FVDFFATYKATLPAIGRLMKVCRARVVPLFPIYDGKTHRLTIQVRPPMDDLLEADDHT
                     IERRMNEEVEIFVGPRPEQYTWILKLLKTRKPGEIQPYKRKDLYPIK"
     gene            complement(11943..13385)
                     /gene="pyk"
                     /locus_tag="OO357_002180"
     CDS             complement(11943..13385)
                     /gene="pyk"
                     /locus_tag="OO357_002180"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HCR5887119.1"
                     /translation="MSKRLRRTKIVTTLGPATDRDNNLEKVIAAGANVVRMNFSHGSP
                     EDHKMRADKVREIAAKLGRHVAILGDLQGPKIRVSTFKEGKVFLNIGDKFLLDANLGK
                     GEGDKEKVGIDYKGLPTDVVPGDILLLDDGRVQLKVLEVQGMKVFTEVTVGGPLSNNK
                     GINKLGGGLSAEALTEKDKADIKTAALIGVDYLAVSFPRCGEDLNYARRLARDAGCDA
                     KIVAKVERAEAVCSQDAMDDIILASDVVMVARGDLGVEIGDPELVGIQKALIRRARQL
                     NRAVITATQMMESMITNPMPTRAEVMDVANAVLDGTDAVMLSAETAAGQYPSETVAAM
                     ARVCLGAEKIPSINVSKHRLDVQFDNVEEAIAMSAMYAANHLKGVTAIITMTESGRTA
                     LMTSRISSGLPIFAMSRHERTLNLTALYRGVTPVPFDSANDGVAAASEAVNLLRDKGY
                     LMSGDLVIVTQGDVMSTVGSTNTTRILTVE"
     gene            complement(13513..14382)
                     /gene="hexR"
                     /locus_tag="OO357_002181"
                     /gene_synonym="yebK"
     CDS             complement(13513..14382)
                     /gene="hexR"
                     /locus_tag="OO357_002181"
                     /gene_synonym="yebK"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator HexR"
                     /protein_id="HCR5887120.1"
                     /translation="MNMLEKIQSQLEHLSKSERKVAEVILASPDNAIHSSIAALALEA
                     NVSEPTVNRFCRSMDTRGFPDFKLHLAQSLANGTPYVNRNVNEDDSVESYTGKIFESA
                     MATLDHVRHSLDKSAINRAVDLLTQAKKIAFFGLGSSAAVAHDAMNKFFRFNVPVVYS
                     DDIVLQRMSCMNCSDGDVVVLISHTGRTKNLVELAQLARENDAMVIALTSAGTPLARE
                     ATLAITLDVPEDTDIYMPMVSRLAQLTVIDVLATGFTLRRGAKFRDNLKRVKEALKES
                     RFDKQLLNLSDDR"
     gene            14719..16194
                     /gene="zwf"
                     /locus_tag="OO357_002182"
     CDS             14719..16194
                     /gene="zwf"
                     /locus_tag="OO357_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HCR5887121.1"
                     /translation="MAVTQTAQACDLVIFGAKGDLARRKLLPSLYQLEKVGQLNPDTR
                     IIGVGRADWDKAAYTKVVREALETFMKETIDEGLWDTLSARLDFCNLDVNDTAAFNRL
                     GAMLDQKNRITINYFAMPPSTFGAICKGLGEAKLNAKPARVVMEKPLGTSLATSQEIN
                     DQVGEYFEECQVYRIDHYLGKETVLNLLALRFANSLFVNNWDNRTIDHVEITVAEEVG
                     IEGRWGYFDKAGQMRDMIQNHLLQILCMIAMSPPSDLSADSIRDEKVKVLKSLRRIDR
                     SNVREKTVRGQYTAGFAQGKKVPGYLEEEGANKSSNTETFVAIRVDIDNWRWAGVPFY
                     LRTGKRLPTKCSEVVVYFKTPELNLFKESWQDLPQNKLTIRLQPDEGVDIQVLNKVPG
                     LDHKHNLQITKLDLSYSETFNQTHLADAYERLLLETMRGIQALFVRRDEVEEAWKWVD
                     SITEAWAMDNDAPKPYQAGTWGPVASVAMITRDGRSWNEFE"
     gene            16429..18240
                     /gene="edd"
                     /locus_tag="OO357_002183"
     CDS             16429..18240
                     /gene="edd"
                     /locus_tag="OO357_002183"
                     /EC_number="4.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydratase"
                     /protein_id="HCR5887122.1"
                     /translation="MNPQLLRVTNRIIERSRETRSAYLARIEQAKTSTVHRSQLACGN
                     LAHGFAACQPENKASLKSMLRNNIAIITSYNDMLSAHQPYEHYPEIIRKALHEANAVG
                     QVAGGVPAMCDGVTQGQDGMELSLLSREVIAMSAAVGLSHNMFDGALFLGVCDKIVPG
                     LTMAALSFGHLPAVFVPSGPMASGLPNKEKVRIRQLYAEGKVDRMALLESEAASYHAP
                     GTCTFYGTANTNQMVVEFMGMQLPGSSFVHPDSPLRDALTAAAARQVTRMTGNGNEWM
                     PIGKMIDEKVVVNGIVALLATGGSTNHTMHLVAMARAAGIQINWDDFSDLSDVVPLMA
                     RLYPNGPADINHFQAAGGVPVLVRELLKAGLLHEDVNTVAGFGLSRYTLEPWLNNGEL
                     DWREGAEKSLDSNVIASFEQPFSHHGGTKVLSGNLGRAVMKTSAVPVENQVIEAPAVV
                     FESQHDVMPAFEAGLLDRDCVVVVRHQGPKANGMPELHKLMPPLGVLLDRCFKIALVT
                     DGRLSGASGKVPSAIHVTPEAYDGGLLAKVRDGDIIRVNGQTGELTLLVDEAELAARE
                     PHIPDLSASRVGTGRELFSALREKLSGAEQGATCITF"
     gene            18277..18918
                     /gene="kdgA"
                     /locus_tag="OO357_002184"
                     /gene_synonym="eda"
     CDS             18277..18918
                     /gene="kdgA"
                     /locus_tag="OO357_002184"
                     /gene_synonym="eda"
                     /EC_number="4.1.2.14"
                     /EC_number="4.1.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 4-hydroxy-2-oxoglutarate
                     aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase"
                     /protein_id="HCR5887123.1"
                     /translation="MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVL
                     EVTLRTECAVDAIRAIAKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLK
                     AATEGTIPLIPGISTVSELMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCP
                     TGGISPANYRDYLALKSVLCIGGSWLVPADALEAGDYDRITKLAREAVEGAKL"
     gene            complement(18974..20152)
                     /gene="purT"
                     /locus_tag="OO357_002185"
     CDS             complement(18974..20152)
                     /gene="purT"
                     /locus_tag="OO357_002185"
                     /EC_number="2.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate-dependent phosphoribosylglycinamide
                     formyltransferase"
                     /protein_id="HCR5887124.1"
                     /translation="MTLLGTALRPAATRVMLLGSGELGKEVAIECQRLGVEVIAVDRY
                     ADAPAMHVAHRSHVINMLDGDALRRVVELEKPHYIVPEIEAIATDMLIQLEEEGLNVV
                     PCARATKLTMNREGIRRLAAEELQLPTSTYRFADSKSLFREAVAAIGYPCIVKPVMSS
                     SGKGQTFIRSAEQLAQAWEYAQQGGRAGAGRVIVEGVVKFDFEITLLTVSAVDGVHFC
                     APVGHRQEDGDYRESWQPQQMSPLALERAQEIARKVVLALGGYGLFGVELFVCGDEVI
                     FSEVSPRPHDTGMVTLISQDLSEFALHVRAFLGLPVGGIRQYGPAASAVILPQLTSQN
                     VTFDNVQNAVGADLQIRLFGKPEIDGSRHLGVVLATSESVVDAIERAKHAAGQVKVQG
                     "
     gene            20286..20576
                     /gene="yebG"
                     /locus_tag="OO357_002186"
                     /pseudo
     CDS             20286..20576
                     /gene="yebG"
                     /locus_tag="OO357_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310585.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA damage-inducible protein YebG"
     gene            20643..20999
                     /locus_tag="OO357_002187"
     CDS             20643..20999
                     /locus_tag="OO357_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YebF"
                     /protein_id="HCR5887125.1"
                     /translation="MKKRGAFLGRLLVSACASVFAANNETSKSVTFPKCEGLDAAGIA
                     ASVKRDYQQNRVARWADDQKIVGQADPVAWVSLQDIQGKDDKWSVPLTVRGKSADIHY
                     QVCVDCKAGMAEYQRR"
     gene            21326..21985
                     /locus_tag="OO357_002188"
     CDS             21326..21985
                     /locus_tag="OO357_002188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance TerB family protein"
                     /protein_id="HCR5887126.1"
                     /translation="MANWLNQLQSLLGQSSSSTSSSADQGLGKLLVSGALGGLAGLLV
                     ANKSARKLLTKYGTNALLVGGGAVAGTVLWNKYKDKIRAAHQDEPQFGAQSTPLDERT
                     ERLILALVFAAKSDGHIDANERAAIDQQLREAGVEEQGRVLIEQAIEQPLDPQRLATG
                     VRNEEEALEIYFLSCAAIDIDHFMERSYLNALGDALKIPQDVREGIERDLEQQKRTLA
                     E"
     gene            22195..24255
                     /gene="ptrB"
                     /locus_tag="OO357_002189"
     CDS             22195..24255
                     /gene="ptrB"
                     /locus_tag="OO357_002189"
                     /EC_number="3.4.21.83"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase B"
                     /protein_id="HCR5887127.1"
                     /translation="MLPKAARIPHAMTLHGDTRIDNYYWLRDDTRSQPKVLDYLQQEN
                     SYGHRVMASQQALQDRILKEIIDRIPQREVSAPYIKNGYRYRHIYEPGCEYAIYQRQS
                     AFSEEWDEWETLLDANKRAAHSEFYSMGGMAITPDNTIMALAEDFLSRRQYGIRFRNL
                     ETGNWYPELLDNVEPSFVWANDSWTFYYVRKHPVTLLPYQVWRHAIGTPASQDTLIYE
                     EKDDTYYVSLHKTTSKHYVVIHLASATTSEVRLLDAEMADAEPFVFLPRRKDHEYSLD
                     HYQHRFYLRSNRHGKNFGLYRTRMRDEQQWEELIPPRENIMLEGFTLFTDWLVVEERQ
                     RGLTSLRQINRKTREVIGIAFDDPAYVTWIAYNPEPETARLRYGYSSMTTPDTLFELD
                     MDTGERRVLKQTEVPGFDAANYRSEHLWIVARDGVEVPVSLVYHRKHFRKGHNPLLVY
                     GYGSYGASIDADFSFSRLSLLDRGFVYAIVHVRGGGELGQQWYEDGKFLKKKNTFNDY
                     LDACDALLKLGYGSPSLCYAMGGSAGGMLMGVAINQRPELFHGVIAQVPFVDVVTTML
                     DESIPLTTGEFEEWGNPQDPQYYEYMKSYSPYDNVTAQAYPHLLVTTGLHDSQVQYWE
                     PAKWVAKLRELKTDDHLLLLCTDMDSGHGGKSGRFKSYEGVAMEYAFLVALAQGTLPA
                     TPAD"
     gene            complement(24252..24914)
                     /gene="exoX"
                     /locus_tag="OO357_002190"
     CDS             complement(24252..24914)
                     /gene="exoX"
                     /locus_tag="OO357_002190"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease X"
                     /protein_id="HCR5887128.1"
                     /translation="MLRIIDTETCGLQGGIVEIASVDVIDGKIVNPMSLLVRPDRPIS
                     PQAMAIHRITEAMVADKPWIEDVIPHYYGSEWYVAHNASFDRRVLPEMPGEWICTMKL
                     ARRLWPGLKYSNMALYKTRKLNVQTPLGLHHHRALYDCYITAALLIDIMNTSGWTAEQ
                     MADITGRPSLMTTFTFGKYRGKAVSDVAERYPGYLRWLFNNLDSMSPELRLTLKHYLE
                     NT"
     gene            complement(24938..25594)
                     /locus_tag="OO357_002191"
                     /pseudo
     CDS             complement(24938..25594)
                     /locus_tag="OO357_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416357.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
     gene            complement(25696..25926)
                     /gene="holE"
                     /locus_tag="OO357_002192"
     CDS             complement(25696..25926)
                     /gene="holE"
                     /locus_tag="OO357_002192"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit theta"
                     /protein_id="HCR5887129.1"
                     /translation="MLKNLAKLDQTEMDKVNVDLAAAGVAFKERYNMPVIAEAVEREQ
                     PEHLRSWFRERLIAHRLASVNLSRLPYEPKLK"
     gene            26065..26439
                     /gene="yobA"
                     /locus_tag="OO357_002193"
     CDS             26065..26439
                     /gene="yobA"
                     /locus_tag="OO357_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopC domain-containing protein YobA"
                     /protein_id="HCR5887130.1"
                     /translation="MASTARSLRYALAILTTSLVTPSVWAHAHLTHQYPAANAQVTAA
                     PQAITLNFSEGVETGFSGAKITGPKNENIKTLPAKRNEQDQKQLIVPLADSLKPGTYT
                     VDWHVVSVDGHKTKGHYTFSVK"
     gene            26443..27315
                     /gene="copD"
                     /locus_tag="OO357_002194"
                     /pseudo
     CDS             26443..27315
                     /gene="copD"
                     /locus_tag="OO357_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310577.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
     gene            27328..27669
                     /locus_tag="OO357_002195"
     CDS             27328..27669
                     /locus_tag="OO357_002195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebY family protein"
                     /protein_id="HCR5887131.1"
                     /translation="MMKKSILAFLLLTSSAAALAAPQVITVSRFEVGKDKWAFNREEV
                     MLTCRPGNALYVINPSTLVQYPLNDIAQKEVASGKTNAQPISVIQIDDPNNPGEKMSL
                     APFIERAEKLC"
     gene            complement(28062..29139)
                     /locus_tag="OO357_002196"
                     /pseudo
     CDS             complement(28062..29139)
                     /locus_tag="OO357_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001189081.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage integrase Arm DNA-binding
                     domain-containing protein"
     gene            complement(29105..29386)
                     /locus_tag="OO357_002197"
     CDS             complement(29105..29386)
                     /locus_tag="OO357_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HCR5887132.1"
                     /translation="MNLVTLKTWGKLRYPDNPPSISTLRRWARNGNIYPAPELHGRSY
                     RVVPEAFYINPNKVDTDITHHQPNGRQGRDSPLMEKLKHAAEKIRSQFA"
BASE COUNT         7434 a         7231 c         7566 g         7225 t
ORIGIN      
        1 tgttttaatg cttcggcgta atcggcagac cagccaactc gcaccagaaa cacgggctga
       61 ccgctggcgc gaaatttcgc cgccagcttc ccggcgcgat taactacctc atcggcagta
      121 tgtggaccac cggcaaaagg taagatgcct tcttgtaaat cgatcaccac caacgcagtg
      181 gttttagcat taagttcaag catgctgact cctgaaaaaa caaaatcgtg cctcacacct
      241 taatgcgatt agccgatggc ggaacgacaa attttgttaa tttttatgag ttcgggcaac
      301 acctttatag caaactggcg tagagtctga tttcaggccc gatataaagt ggtaacgagg
      361 cgttccagcg cgggccaggc tcttacggtg cggcagaatt tccagtataa tagccgcctt
      421 ttttcattca gttgtgacat acagctaacg ctgcgacttg gtcacctgcg cagcaggggg
      481 catccgccgg cagctaagtt aagggatatc tcatgcgtac agaatattgt ggacagctcc
      541 gtttgtccca cgtggggcag caggtgactc tgtgtggttg ggtcaaccgt cgtcgtgatc
      601 ttggtagcct gatcttcatc gatatgcgcg accgcgaagg tatcgtgcag gtatttttcg
      661 atccggatcg tgcggacgcg ttaaagctgg cctctgaact gcgtaatgag ttctgcattc
      721 aggttacggg caccgtacgt gcgcgtgacg aaaaaaatat taaccgcgat atggcgaccg
      781 gcgaaattga agtgctggcg tcctcgctga ctatcatcaa ccgcgcagat gttctgccgc
      841 ttgactctaa ccacgtcaac accgaagaag cgcgtctgaa ataccgctac ctcgacctgc
      901 gtcgcccgga aatggcgcaa cgcctgaaaa cccgcgctaa aatcaccagc ctggtgcgcc
      961 gttttatgga tgaccacggc ttcctcgaca tcgaaactcc gatgctgacc aaagccacgc
     1021 cggaaggcgc gcgtgactac ctggtgcctt ctcgtgtgca caaaggtaaa ttctacgcac
     1081 tgccgcaatc cccacagttg ttcaaacagc tgctgatgat gtccggtttt gaccgttact
     1141 atcagatcgt taaatgcttc cgtgacgaag acctgcgcgc tgatcgtcag cctgaattta
     1201 ctcagatcga tgtggaaact tctttcatga ccgcgccgca agtgcgtgaa gtgatggaag
     1261 cgctggtgcg tcatttgtgg ctggaagtga agggcgtgga tctgggcgat ttcccggtaa
     1321 tgacctttgc ggaagcagaa cgccgttatg gttctgataa accggatctg cgtaacccga
     1381 tggaactgac cgacgttgcc gatctgctga aatctgttga gtttgcagta tttgcggggc
     1441 cagcgaacga tccgaaaggc cgtgttgccg ctctacgtgt tccgggtggc gcatcgctga
     1501 cccgtaagca gatcgacgaa tacggtaact tcgttaaaat ctacggcgcg aaaggtctgg
     1561 cttacatcaa agttaacgaa cgcgcgaaag gtctggaagg tatcaacagc ccggtagcga
     1621 agttccttaa tgcagaaatc atcgaagcca tcctggatcg tactgccgcg caagatggcg
     1681 atatgatttt cttcggtgcc gacaacaaga aaattgttgc cgacgggatg ggcgcactgc
     1741 tcctgaaagt gggtaaagac cttggtctga ccgacgaaag caaatgggca ccgctgtggg
     1801 ttatcgactt cccgatgttt gaagacgacg gtgaaggcgg cctgacggca atgcaccatc
     1861 cgttcacctc accgaaagat atgactgctg cagaactgaa agctgcaccg gaaaatgcgg
     1921 tggcgaacgc ttacgatatg gtcatcaatg gttacgaagt gggcggtggt tcagtacgta
     1981 tccataatgg tgatatgcag cagacggtgt ttggtattct gggtatcaac gaagaggaac
     2041 agcgcgagaa attcggcttc ctgctcgacg ccctgaaata cggtactccg ccgcacgcag
     2101 gtctggcatt cggtcttgac cgtctgacca tgctgctgac tggcaccgac aatatccgtg
     2161 acgttatcgc cttcccgaaa accacggcgg cagcgtgtct gatgactgaa gcaccgagct
     2221 ttgctaaccc gactgcactg gctgagctga gcattcaggt tgtgaagaag gctgagaata
     2281 actgatatga ctcaaataca cgaaatcatt cgcgttgcat caaggcggca actgagtgaa
     2341 ctcccatgag catagataac tatgtgaatg ggatgagcga aggcagtcaa cgaagaggca
     2401 gcgtgaagga taaagtgtat aagcgtcccg tttcgatctt agtggtcatc tatgcacaag
     2461 atacgaaacg ggtgctgatg ttgcagcggc gtgacgatcc cgatttctgg cagtcggtaa
     2521 ccggcagcgt ggaagagggt gaaaccgcgc cgcaagctgc catgcgcgaa gtaaaggaag
     2581 aggttaccat tgatgttgtc gctgaacaac tgaccttaat tgactgtcag cgcacggtag
     2641 agtttgaaat tttttcacat ttacgtcatc gctatgcgcc gggcgtgacg cgtaatacgg
     2701 aatcatggtt ctgtcttgcg cttccgcacg agcggcagat cgttttcact gaacatctgg
     2761 cttacaagtg gcttgatgcg cctgctgcgg cggcgctcac taagtcctgg agcaaccggc
     2821 aggcgattga acagtttgta attaacgctg cctgaacagg cagggctatc tggagatatt
     2881 tttatggcag gtcatagtaa atgggccaac accagacatc gtaaagctgc gcaggatgct
     2941 aagcgcggta aaatcttcac taaaatcatt cgtgagctgg taaccgcggc taagctgggc
     3001 ggtggcgatc cggacgctaa cccgcgtctg cgtgcggcgg tggataaagc actgtctaac
     3061 aacatgaccc gtgacacact gaaccgcgca attgcacgtg gtgtgggcgg tgatgatgat
     3121 gcaaacatgg aaaccatcat ctacgaaggt tacggtcctg gcggcacggc aatcatgatt
     3181 gaatgtctgt ctgacaaccg caaccgtacc gttgctgaag tgcgtcacgc attcagcaaa
     3241 tgtggcggta acctcggcac tgacggttcc gtagcttatc tgttcagcaa aaaaggcgtg
     3301 atctccttcg agaaaggcga cgaagacacc atcatggaag cagctctgga agcaggtgct
     3361 gaagacgttg tgacatatga tgacggcgcg attgatgtct acactgcatg ggaagaaatg
     3421 ggtaaagtgc gcgacgctct ggaagcggca ggtctgaaag cagacagcgc ggaagtttcc
     3481 atgatcccgt ccaccaaagc tgatatggat gcagaaaccg cgccgaaact gatgcgtctg
     3541 atcgatatgc tggaagattg cgacgacgtg caggaagttt accataacgg tgaaatctct
     3601 gatgaggttg cagcgactct ctgatgatgc ctgctaaaca gcaaaacgga gacgcgtgat
     3661 ggctattatt ctcggcattg acccgggttc gcgcgtgacc ggctacggcg tcatccgcca
     3721 ggtaggtagg caactgtcct acctgggtag cggatgcata cgcaccaaag tggatgattt
     3781 accgtctcgt ctgaagctca tctatgcggg cgtgacggaa atcatcaccc agttccagcc
     3841 tgattatttc gccattgaac aagtctttat ggcgaagaac gctgactcag ccctgaaact
     3901 gggccaggcg cgcggcgtgg cgattgttgc ggcggtgaat caggaattgc cggtatttga
     3961 atacgcggca cgtcaggtaa agcaaacggt ggtaggtatg ggtagtgccg aaaaaagcca
     4021 ggtgcagcat atggtccgca ccttgctgaa actgcccgct aatccacagg cggatgccgc
     4081 cgatgcgctg gcgattgcca tcacccactg ccacgttagt cagaatgcga tgcagatgag
     4141 cgaatcgcgg ctgaacctga cgagagggcg actgcgttaa gttataccgc ctcggtcagt
     4201 tccggctgag gcgtttcaac tccctccgcg tgaatcaaca ccaaacccgg atgatgccac
     4261 aaactggctg gcgtgacggt tttacgctcc cacggaatag aacctaagaa ccagaatttc
     4321 cagaaagtga gttttgcatt cggattgcta tttaacagtt gtccaaacgt ctctatttca
     4381 cccaccggaa tacatagcgc ctctttataa atatcaaaga caaattttga acagaactgg
     4441 cgcgaagatt cgtatttaaa acccgtgtgg taaagtttgc gtagccggga gggaacctgt
     4501 tcaacaattc gttgtttttg ttgttctgtc agcccagcgt ctaatcgctt tatagcatag
     4561 cgttgattag acgaacgttt aataaaacga gatagcgtgg tgatggttga aaggggaacc
     4621 cggctttctg caaccaggaa gtcttcaccg ttatgaccga taatgatccc gacgtgatta
     4681 ctccagcaat ttgatgcagc tgatatttga ccaaataagg cagcacctat acatgtaaag
     4741 acgatatctc caatttcata ttcagcaggg taattaatat tcacgttgtc attccattga
     4801 aatagataca aatatacgta atagcataaa aaaattattt ttcaggagtc gcaatgtata
     4861 tttttattac gcatttcttc actgaatatg taatattcaa atatttgctt ccaatataac
     4921 ctgttgaata aattatactg tgccattttt cagatcatcg ggacacctcg caagttttct
     4981 tcatccttcg ctggatatct atccagcatt tttttatcat acagcattat ctttgattca
     5041 ttacgcagga gcgtcatgtg ataggcagac tcagaggcat catcattgaa aaacaacccc
     5101 cgctggtgtt aattgaagtg ggcggcgtag gctatgaagt gcatatgccg atgacctgtt
     5161 tttatgaact ccctgaagcg ggtcaggaag cgatcgtttt cacccacttt gtggtgcgtg
     5221 aagacgcgca actgctgtac ggttttaaca ataaacaaga acgcacgttg ttcaaagagt
     5281 tgatcaaaac caacggcgtc ggcccgaagt tggccctggc gatcctctcc ggaatgtcag
     5341 cgcagcagtt cgttaatgcc gttgagcgtg aagaagtggg ggcactggtg aaactgccgg
     5401 gtattggcaa aaaaaccgcc gaacgcttga ttgttgaaat gaaagaccga tttaaaggtt
     5461 tgcatggcga tctctttacg ccagccgccg acctggtact cacgtctcct gctagcccgg
     5521 cgaccgacga tgctgaacaa gaagcagttg ccgcgctggt ggcgctaggc tataaaccac
     5581 aagaagcaag ccgcatggtg agcaaaatcg ctcgccctga caccagcagt gaaacattaa
     5641 ttcgcgaagc cctacgcgcc gcgttatgag gtaaaggatg attgaagcag accgtctgat
     5701 ttctgccggt accactttgc cggaagatgt agcagatcgc gccattcgcc ctaaattact
     5761 ggaagagtat gttggtcagc cgcaggttcg ttcacagatg gagattttca tcaaagcagc
     5821 gaaactgcgc ggcgatgccc tcgatcattt gttgattttt ggtcctccgg ggttgggtaa
     5881 aactacgctt gccaacattg tcgccaatga aatgggcgtt aatttacgca cgacttctgg
     5941 tccggtgctg gaaaaggcgg gcgatttggc tgcgatgctc actaaccttg aaccgcatga
     6001 cgtgctgttt attgatgaga tccaccgttt atcgccagtt gttgaagaag tgctgtaccc
     6061 ggcaatggaa gactaccaac tggatatcat gattggtgaa ggtccggcgg cacgctccat
     6121 taaaattgat ttgccgccgt ttaccctgat tggcgcaacc acgcgcgcag gttcgctgac
     6181 atcaccgttg cgcgaccgtt ttggtattgt gcaacgtctg gagttttatc aggtgccgga
     6241 tctgcaatat atcgtcagtc gcagtgcacg ctttatgggg cttgagatga gtgatgacgg
     6301 tgcgcaggaa gtagctcgtc gcgctcgcgg tacgccacgt attgccaacc gtctgctgcg
     6361 tcgagtgcgt gatttcgccg aagtgaagca cgatggcacc atctcggcag atatcgctgc
     6421 tcaggcgctg gatatgttga atgtcgatgc tgaaggtttc gattatatgg accgcaaatt
     6481 gttgctggcg gtaatcgata agttctttgg tgggccggta ggtctggata acctggcggc
     6541 agccattggc gaagaacgtg aaaccattga agatgtactg gaaccttatt tgattcagca
     6601 aggctttttg cagcgtacac cgcgcgggcg tatggcgacg acgcgggcgt ggaatcactt
     6661 tggcataacg ccgccagaaa tgccgtaagt cggattggtc gctcgcgccg catccgacac
     6721 tggcagtgtg tctgatgcga cactgttcgc gtcttatcag gccagccaga gatagcgaac
     6781 cgtaggtcgg ataaggcgct cgcgccgcat ccgacaaatg tgttcagcga tagattagct
     6841 ggcctgcttt ttcatcatac tgataataaa taacagtgcc gcacatagca ccaccgacgg
     6901 acccgccggc gtatcgtaaa acgcggaaaa ggttaaaccg ccagttactg ccaccatccc
     6961 caccaaaaca gcgacaccag ccatctgttc cggcgtacgg gcaaagcgac gcgcagtagc
     7021 tgcaggaata atcagcaacg aagtaataat caacgcaccg acaaatttca tcgctacacc
     7081 aatcgtcaat gccgtcacca gcatcaacaa caatttcact tgctgtaatt tcacaccatc
     7141 aacaaacgcc agatccgggc taatcgtcat cgacagcaaa ttgcgccatt gccagaacaa
     7201 aatagccacc acgatgacca cgccaatcgc aatagagatg agatcttctg gcgtcactgc
     7261 cagtaaatca ccgaacaggt aagccatcaa atcaacacga atgttagaca tcagactaac
     7321 gaccactagg cccagcgaca gggcactgtg cgccataatc cctaataacg tgtcgatcgc
     7381 cagctgtgga cgcttctcca gccataccag accgcccgcc agcagcagcg taacggcaat
     7441 caccgcatag aatggattca cgtccagcaa caaaccaaac gcgacaccaa gtaatgaggc
     7501 atgagccagc gtatcaccga aataagacat acgacgccag actacaaacg aacccagcgg
     7561 acccgcggca caggcgagca tgatcccggc taaccaaccg ggaaataata attcaatcat
     7621 gagcgatcat ttccccgacg caaaacaatt cgtccctgta aatcgtgacg atgattatga
     7681 tgatggcgat agatacccag ttgttcagca ccacgaggac caaacattga aataaactcc
     7741 ggatgcaggg aaacaacttc cggtgtgccg gaacaacaaa tgtggtgatt caggcacagc
     7801 acttcatcgg tttttgccat caccagatgc agatcgtgag aaaccattaa aacgccacaa
     7861 tccagttcac gacgcaactg gtcaataagg tcatataacg ccacttggcc atttacatcc
     7921 acgccttgag tgggttcatc cagcactaat aattgcgggc aatttaacaa tgctcgcgct
     7981 aatagtacac gctgcgtttc gccaccagag agcttttgca tcggtgcgtt aatcaggtgc
     8041 ccggcctgga cacgtttcag tgcaggcaaa atatcttctt tatgtgtacc agggcgtaag
     8101 cgtaaaaaac ggtttacggt cagtggcaac gtggtgtcga gatacagctt ctgcggtaca
     8161 tagccgatgc gcagttttcc gttgcgcttg ataacccctt catcgggtgt taccagcccg
     8221 agcactaccc gtaccagtgt cgacttacct gcgccatttg gcccaagtaa agtcaaaatt
     8281 tttccaggtt taagttccag cgacacatca gagaggacgc ggcgttggcc aaaagaaacc
     8341 gagacatttt ccagggaaac cagacttgtc atgttaattt tagtcttgca gtagtcatga
     8401 aatgttataa tatcacactt ctcatattca ttacgatgat tggtcgcatt atgttacata
     8461 aaaaaacgct tcttttcgca gcattatccg ccgctctctg gggaggtgca acacaggccg
     8521 cagatgccgc cgttgtcgct tcgcttaaac ccgttgggtt catcgcttct gccattgctg
     8581 atggtgtaac agaaacggag gttttactgc ctgacggcgc ttcagaacat gattattcac
     8641 tgcgtccatc ggatgtaaaa cgcttacaga acgcggactt agtcgtttgg gttggcccgg
     8701 agatggaagc gtttatgcaa aaaccggtaa gcaaattacc agaagcgaag caggtaacga
     8761 ttgcgcagct tgaagatgtg aaaccgctac tgatgaaaag tattcacggc gatgatgatg
     8821 atcacgacca cgcggaaaaa agtgacgaag atcaccatca cggcgatttc aacatgcatc
     8881 tttggctttc cccagagata gcgcgggcta cagcggttgc aatccatgga aaattagtgg
     8941 aacttatgcc gcaaagtcga gccaaacttg acgccaacct gaaggatttt gaggcacaat
     9001 tggcctcaac cgaaacgcag gttggtaacg agctcgcgcc gctcaagggg aaaggttatt
     9061 tcgtttttca cgatgcttac ggctatttcg aaaaacagtt cggactgaca ccgcttggtc
     9121 attttaccgt taaccctgag attcaacctg gcgcgcagcg tttacatgaa ataagaacac
     9181 agttggttga gcaaaaagca acctgcgttt ttgctgagcc acagttcagg ccagcggtcg
     9241 ttgagagcgt cgcacgaggg acatccgttc gtatgggaac gttggatccc ctggggacga
     9301 atatcaaact gggtaaaaca agctattcag aattcctgag tcaattagcc aaccagtatg
     9361 cgagctgcct gaaaggagat taatgaggaa gtgattacgt gcaacagata gcccgctctg
     9421 tcgccctggc gtttaataat ttaccgcgac cacaccgcgt tatgttgggg tcgctcaccg
     9481 ttcttactct ggccgtcgct gtctggcggc cctatgttta tcaccgtgat gccacgccaa
     9541 ttgtcaaaac cattgagctg gaacagaacg aaattcgttc gctcttacct gaagccagtg
     9601 agccgattga tcaagctgca caagaagatg aagccattcc ccaggacgaa ctggatgaca
     9661 aaatcgccgg tgaagcgggc gtgcatgaat atgttgtttc cactggcgat acgctaagca
     9721 gcattctcaa tcagtatggt attgatatgg gtgatatcac ccaacttgct gctgccgaca
     9781 aagaattgcg taacctgaaa atcggtcaac aactctcctg gacattaacc gcggacggcg
     9841 aactgcagcg cctcacctgg gaagtgtctc gtcgtgaaac ccgaacctat gaccgtactg
     9901 ccgctaacgg ttttaaaatg accagcgaaa tgcagcaagg agagtgggtt aacaatctgc
     9961 tgaaaggtac cgtcggaggg agctttgttg ccagcgccag aaacgccggt ttaaccagcg
    10021 ccgaagtgag cgcagtgatt aaagccatgc agtggcaaat ggatttccgc aaactgaaaa
    10081 aaggcgatga atttgcggta ttaatgtctc gagaaatgct tgatggtaaa cgtgagcaaa
    10141 gccagctgct gggcgtacgt ttgcgttcag aaggtaaaga ttattacgca atccgcgctg
    10201 aggatggcaa attctacgat cgtaacggta ctggtctggc gaaaggattc ttgcgattcc
    10261 cgacggcgaa acagttccgt atctcgtcta actttaaccc gcgtcgaact aatccggtga
    10321 caggtcgcgt tgcaccacac agaggtgttg atttcgccat gccacaaggt acgccagtgc
    10381 tttcagtggg tgacggtgaa gtggtggttg ccaaacgcag tggcgcagca ggttattatg
    10441 tggctattcg tcatggtcgc agctacacca cgcgttatat gcacttgcgc aagattctgg
    10501 tgaaaccggg acagaaggtg aaacgtggcg accgtatcgc gctttccggt aataccggac
    10561 gttcaactgg gccgcatctg cactatgaag tatggataaa ccagcaggcc gtaaacccgc
    10621 tgacggcaaa actgccgcgt accgaagggc tgaccggctc cgatcgtcgc gaattcctgg
    10681 cgcaggccaa agagattgtg ccgcagctac ggtttgatta attaacatcc attcgcagcc
    10741 ggtacgcagt cagtaccggc tttttttatt tggtgcgggg caagttgtgc cgctacacta
    10801 tcaccagatt gatttttgcc ttatccgaaa ctggaaaagc atggaaacga aaaaaaataa
    10861 tagcgaatac attcctgagt ttgataaatc ctttcgccac ccgcgctact ggggagcatg
    10921 gctgggtgta gcagcgatgg cgggtatcgc tttaacgccg ccaaagttcc gtgatcccat
    10981 tctggcacgg ctgggacgtt ttgccggacg actgggaaaa agctcacgcc gtcgtgcgtt
    11041 aatcaatctg tcgctctgct ttccagaacg tagtgaagct gaacgcgaag cgattgttga
    11101 tgagatgttt gccaccgcgc cgcaagcgat ggcaatgatg gctgagttgg caatacgcgg
    11161 gccggagaaa attcagccgc gcgttggctg gcaagggctg gagatcatcg aagagatgcg
    11221 gcgtaataac gagaaagtta tctttctggt gccgcacggt tgggccgtcg atattcctgc
    11281 catgctgatg gcctcgcaag ggcagaaaat ggcagcgatg ttccataatc agggcaaccc
    11341 ggtttttgat tatgtctgga acacggtgcg tcgtcgcttt ggcggtcgtc tgcatgcgag
    11401 aaatgacggt attaaaccat tcatccagtc ggtacgtcag gggtactggg gatattattt
    11461 acccgatcag gatcatggcc cagagcacag cgaatttgtg gatttctttg ccacctataa
    11521 agcgacgttg cccgcgattg gtcgtttgat gaaagtatgc cgtgcgcgcg ttgtaccgct
    11581 gtttccgatt tatgatggca agacgcatcg tctgacgatt caggtgcgcc caccgatgga
    11641 tgatctgtta gaggcggatg atcatacgat tgagcggcgg atgaatgaag aagtcgagat
    11701 ttttgttggt ccgcgaccag aacaatacac ctggatacta aaattgctga aaactcgcaa
    11761 accgggcgaa atccagccgt ataagcgcaa agatctttat cccatcaaat aaaaaagcct
    11821 ctcgcgagga gaggccttcg cctgatgata agttcaagtt tgcttcagaa tattcgaaat
    11881 ctgttgaact atcattgaac tataggccgg atgtggcgtt ttcgccgcat ccggcaacgt
    11941 acttactcta ccgttaaaat acgcgtggta ttagtagaac ccacggtact catcacgtcg
    12001 ccttgggtga caatcaccag gtcaccagac atcaagtaac ctttatcgcg cagcagatta
    12061 accgcttcgc tggcagctgc tacgccgtca ttagcgctat caaagggcac cggcgtaacg
    12121 ccacgataga gagcagtcag gttcagcgta cgttcatggc gcgacatggc gaaaattggt
    12181 agaccagagc tgatacggga ggtcatcagc gcggtacgac ccgattcggt catggtaatg
    12241 atcgccgtaa cgcccttcag gtggttagct gcgtacattg ccgacatggc aatagcttct
    12301 tccacattgt cgaactgaac gtccagacgg tgtttagaaa cgttgatgct cgggattttt
    12361 tccgcaccca ggcaaacgcg cgccatggct gcaacagttt ctgacggata ctgcccagcg
    12421 gcagtttctg cagacagcat cacagcgtca gtaccatcca gaacggcgtt tgctacgtcc
    12481 atgacttctg cacgcgtcgg catcgggtta gtaatcattg actccatcat ctgggtcgcc
    12541 gtgattaccg ctcggtttag ctgacgcgca cgacggatca acgctttctg aatgccgacc
    12601 agttccgggt cgccaatttc cacaccgagg tcgccacgtg caaccattac cacgtcagag
    12661 gcgaggatga tgtcatccat tgcatcctgg ctgcaaacgg cttccgcacg ttcaaccttg
    12721 gcaacaattt tcgcatcaca tcctgcatcg cgtgccaggc gacgggcata gttcagatct
    12781 tcgccacagc gtgggaagga gacagccagg taatctacgc caatcaacgc cgcagtctta
    12841 atgtctgctt tgtctttttc ggtcagcgct tcagccgaca aaccgccgcc aagtttgttg
    12901 atacctttat tgttggagag gggaccaccg acggtgactt cggtgaacac tttcatgccc
    12961 tgaacttcca gtacttttaa ctggacgcga ccatcgtcca gcagcaggat gtcaccaggc
    13021 acgacgtcag taggcaggcc tttgtagtcg ataccgactt tttctttgtc gccttcacct
    13081 ttacccaggt tggcgtcgag caggaattta tccccaatat tgaggaaaac tttgccttct
    13141 ttaaaggtgg atacacggat tttgggcccc tggaggtcac ccagaatagc cacatgacgc
    13201 cccagttttg cggcaatctc acgaacttta tccgcgcgca ttttgtgatc ttcaggcgag
    13261 ccgtgagaaa agttcatacg tacaacgttg gcacccgccg cgataacttt ttcaagatta
    13321 ttatcgcgat ctgttgctgg gcctaacgtg gtaacgattt ttgttctgcg aagccttttg
    13381 gacatgtaat actccgttga ctgaaacaac caggtgttgc ttgaacatga aatccgaatg
    13441 aaataacgcc gcgatggcga taaattaatc tcattcggat gtcgtataag attaggacag
    13501 tgacagtcgc ttttagcgat cgtcacttaa attaagtaac tgcttatcaa aacgcgattc
    13561 cttcagcgct tccttgaccc gcttcaagtt atctctgaat tttgcaccgc gtcgcaaagt
    13621 aaatcctgtc gccagcacat ctatcacggt cagctgtgca agtcgagaaa ccatgggcat
    13681 ataaatgtca gtatcttccg gtacgtcgag ggtaattgcc agcgttgctt cccgggcgag
    13741 cggggtaccc gcagaggtga gggcaatcac catggcgtcg ttttcgcgtg ccagctgcgc
    13801 cagctcgacc agattttttg ttcttcctgt gtgagaaatc agcaccacca cgtctccgtc
    13861 gctacaattc atacaactca tgcgttgcag cacgatatca tcggagtaca ccaccggaac
    13921 attaaaacga aagaacttat tcatcgcatc gtgggcaacg gcggcagaag agcctaatcc
    13981 gaaaaaggcg attttttttg cctgagtgag caagtcgacg gcgcggttga tggcagattt
    14041 atccagtgaa tgacggacat gatcgagcgt tgccattgcg gactcaaata ttttccccgt
    14101 gtatgattca acgctgtcat cttcattgac gttgcgatta acatagggag tgccattcgc
    14161 cagactttgt gccagatgaa gtttaaaatc aggaaaaccg cgcgtgtcca tgctgcgaca
    14221 gaaacgattc accgtcggtt cgctaacatt ggcttccagc gccagagcag caatactcga
    14281 atggatcgcg ttatcgggcg aagccagaat gacctcggca actttgcgct ctgatttgct
    14341 caaatgttcc agctgagact ggattttttc cagcatattc atgatgtaaa gagactcacg
    14401 ggtaatgacg atttccgcac tgaaagaaat cgaaatgcag ttttgtcaga tattacgcct
    14461 gtgtgccgtg ttaatgacaa aagcagataa aaaagttgtt atttttttca taacatgatc
    14521 agtgtcagat ttttaccgaa tggaaaacga tgattttttt atcagttttg ccgcgctttg
    14581 cgcgcttttc ccgtaatcgc acgggtggat aagcgtttac agttttcgca agctcgtaaa
    14641 agcagtacag tgcaccgtaa gaaaattaca agtataccct ggcttaagta ccgggttagt
    14701 taacttaagg agaatgacat ggcggtaacg caaacagccc aggcctgtga cctggtcatt
    14761 ttcggcgcga aaggcgacct tgcgcgtcgt aaattgctgc cttccctgta tcaactggaa
    14821 aaagtcggtc agctcaaccc ggacacccgg attatcggcg tagggcgtgc tgactgggat
    14881 aaagcggcat ataccaaagt tgtccgcgag gcgctcgaaa ctttcatgaa agaaaccatt
    14941 gatgaaggtt tatgggacac cctgagtgca cgtctggatt tttgtaatct cgatgtcaat
    15001 gacactgctg cattcaaccg tctcggcgcg atgctggatc aaaaaaatcg tatcactatt
    15061 aactactttg ccatgccgcc cagcactttt ggcgcaattt gcaaagggct tggcgaggca
    15121 aaactgaatg ctaaaccggc acgcgtagtc atggagaaac cgctggggac gtcgctggcg
    15181 acctcgcagg aaatcaatga tcaggttggc gaatacttcg aggagtgcca ggtttaccgt
    15241 atcgaccact atcttggtaa agaaacggtg ctgaacctgt tggcgctgcg ttttgctaac
    15301 tccctgtttg tgaataactg ggacaatcgc accattgatc atgttgagat taccgtggca
    15361 gaagaagtgg ggatcgaagg gcgctggggc tattttgata aagccggtca gatgcgtgac
    15421 atgattcaga accacctgct acaaattctc tgcatgattg cgatgtctcc gccatctgac
    15481 ctgagcgcag acagcatccg cgatgaaaaa gtgaaagtac tgaagtccct gcgccgcatc
    15541 gaccgctcca acgtacgcga aaaaaccgta cgcgggcaat atactgcggg cttcgcccag
    15601 ggcaaaaaag tgccgggata tctggaagaa gagggcgcga acaaaagcag caatacagaa
    15661 accttcgtgg cgatccgcgt cgacattgat aactggcgct gggccggtgt gccattctac
    15721 ctgcgtactg gtaaacgtct gccgaccaaa tgttctgaag tcgtggtcta tttcaaaaca
    15781 cctgaactga atctgtttaa agagtcgtgg caggatctgc cgcagaataa actgactatc
    15841 cgtctgcaac ctgatgaagg cgtggatatc caggtactga ataaagttcc tggccttgac
    15901 cacaaacata acctgcaaat caccaagctg gatctgagct attcagaaac ctttaatcag
    15961 acgcatctgg cggatgccta tgaacgtttg ctgctggaaa ccatgcgtgg tattcaggca
    16021 ctgtttgtac gtcgcgacga agtggaagaa gcctggaaat gggtagactc cattactgag
    16081 gcgtgggcga tggacaatga tgcgccgaaa ccgtatcagg ccggaacctg gggacccgtt
    16141 gcctcggtgg cgatgattac ccgtgatggt cgttcctgga atgagtttga gtaatatcag
    16201 cgcttatcct ttatggttat tttaccggta acatgatctt gcacagattg tagaacaatt
    16261 tttacacttt caggcctcgt gcggattcac ccacgaggct ttttttatta cactgactga
    16321 aacgtttttg ccctatgagc tccggttaca ggcgtttcac tcataaatcc tctgaatgaa
    16381 acgcgttgtg aatcatcctg ctctgacaac tcaatttcag gagcctttat gaatccacaa
    16441 ttgttacgcg taacaaatcg aatcattgaa cgttcgcgcg agactcgctc tgcttatctc
    16501 gcccggatag aacaagcgaa aacgtcgacc gttcatcgtt cgcagttggc atgcggtaac
    16561 ctggcacacg gtttcgctgc ctgccagcca gaaaacaaag cctctttgaa aagcatgttg
    16621 cgtaacaata tcgccatcat cacctcctat aacgacatgc tctccgcgca ccagccttat
    16681 gaacactatc cagaaatcat tcgtaaagcc ctgcatgaag cgaatgcggt tggtcaggtt
    16741 gcgggcggtg ttccggcgat gtgtgatggt gtcacccagg ggcaggatgg aatggaattg
    16801 tcgctgctaa gccgcgaagt gatagcgatg tctgcggcgg tggggctgtc ccataacatg
    16861 tttgatggtg ctctgttcct cggtgtgtgc gacaagattg tcccgggtct gacgatggca
    16921 gccctgtcgt ttggtcattt gcctgcggtg tttgtgccgt ctggaccgat ggcaagcggt
    16981 ttgccaaata aagaaaaagt gcgtattcgc cagctttatg ccgaaggtaa agtggaccgc
    17041 atggccttac tggagtcaga agccgcgtct taccatgcgc cgggaacatg tactttctac
    17101 ggtactgcca acaccaacca gatggtggtg gaatttatgg ggatgcagtt gccaggctct
    17161 tcttttgttc atccggattc tccgctgcgc gatgctttga ccgctgccgc tgcgcgtcag
    17221 gttacacgca tgaccggtaa tggtaatgaa tggatgccga tcggtaagat gatcgatgag
    17281 aaagtggtgg tgaacggtat cgttgcactg ctggcgaccg gtggttccac taaccacacc
    17341 atgcacctgg tggcgatggc gcgcgcggcc ggtattcaga ttaactggga tgacttctct
    17401 gacctttctg atgttgtacc gctgatggca cgtctctacc cgaacggtcc ggccgatatt
    17461 aaccacttcc aggcggcagg tggcgtaccg gttctggtgc gtgaactgct caaagcaggc
    17521 ctgctgcatg aagatgtcaa tacggtggca ggctttggtc tgtctcgtta tacccttgaa
    17581 ccatggctga ataatggtga actggactgg cgggaagggg cggaaaaatc actcgacagc
    17641 aatgtgatcg cttccttcga acaacctttc tctcatcatg gtgggacaaa agtgttaagc
    17701 ggtaacctgg gccgtgcggt tatgaaaacc tctgccgtgc cggttgagaa ccaggtgatt
    17761 gaagcgccag cggttgtttt tgaaagccag catgacgtta tgccggcctt tgaagcgggt
    17821 ttgctggacc gcgattgtgt cgttgttgtc cgtcatcagg ggccaaaagc gaacggaatg
    17881 ccagaattac ataaactcat gccgccactt ggtgtattat tggaccggtg tttcaaaatt
    17941 gcgttagtta ccgatggacg actctccggc gcttcaggta aagtgccgtc agctatccac
    18001 gtaacaccag aagcctacga tggcgggctg ctggcaaaag tgcgcgacgg ggacatcatt
    18061 cgtgtgaatg gacagacagg cgaactgaca ctgctggtag acgaagcgga actggctgct
    18121 cgcgaaccgc acattcctga cctgagcgcg tcacgcgtgg gaacaggacg tgaattattc
    18181 agcgccttgc gtgaaaaact gtccggtgcc gaacagggcg caacctgtat cactttttaa
    18241 gacgacaaat ttgtaatcag gcgagagaaa actctgatga aaaactggaa aacaagtgca
    18301 gaatcaatcc tgaccaccgg cccggttgta ccggttatcg tggtaaaaaa actggaacac
    18361 gcggtgccga tggcaaaagc gttggttgct ggtggggtgc gcgttctgga agtgactctg
    18421 cgtaccgagt gtgcagttga cgctatccgt gctatcgcca aagaagtgcc tgaagcgatt
    18481 gtgggtgccg gtacggtgct gaatccacag cagctggcag aagtcactga agcgggtgca
    18541 cagttcgcaa ttagcccggg tctgaccgag ccgctgctga aagctgctac cgaagggact
    18601 attcctctga ttccggggat cagcactgtt tccgaactga tgctgggtat ggactacggt
    18661 ttgaaagagt tcaaattctt cccggctgaa gctaacggcg gcgtgaaagc cctgcaggcg
    18721 atcgcgggtc cgttctccca ggtccgtttc tgcccgacgg gtggtatttc tccggctaac
    18781 taccgtgact acctggcact gaaaagcgtg ctgtgcatcg gtggttcctg gctggttccg
    18841 gcagatgcgc tggaagcggg cgattacgac cgcattacta agctggcgcg tgaagctgta
    18901 gaaggcgcta agctgtaaaa gtcaaaatgc ccgatctagg atcgggcatt tttgtagcgt
    18961 ttttttgccg ggtttaaccc tgtactttta cctgtccggc ggcgtgcttc gcgcgttcaa
    19021 tggcgtcaac aacactctct gaagtagcca gtaccacccc cagatgacgg ctgccatcaa
    19081 tttccggctt accaaataaa cgaatctgca aatctgcgcc tacggcattc tgcacattat
    19141 caaacgtgac attctgactg gtcagttgtg gcagaataac ggcagaagct gcaggaccat
    19201 actgacggat cccgccaaca ggaagtccga ggaaggcacg tacatgcagg gcaaactctg
    19261 agagatcttg agaaattaac gtcaccatcc cggtatcatg tggacgaggg gagacctcac
    19321 tgaaaatcac ctcatcacca cagacaaaca gctcgacacc aaacagacca taaccgccca
    19381 gtgccagcac cactttacgg gcaatctcct gcgcacgttc aagggcaagc gggctcattt
    19441 gctgtggttg ccaggattca cggtagtcgc catcttcctg gcgatgacct actggtgcac
    19501 agaaatggac gccatccacc gcgctgacgg ttagcagggt aatttcgaag tcaaacttaa
    19561 cgacgccttc aacaattacg cggcccgctc cggcgcgacc gccttgctga gcgtattccc
    19621 atgcctgagc aagttgctct gcagagcgaa taaacgtctg ccccttgccg gaagagctca
    19681 tcaccggttt tacaatgcag ggatagccaa tggcggcaac cgcctcgcgg aaaaggcttt
    19741 tgctatcggc aaaacgataa gtggaagtgg gcagctgcag ctcttctgcc gccaggcgac
    19801 ggataccctc gcgattcatc gttaatttcg ttgcgcgagc gcaggggaca acattcagtc
    19861 cttcctcttc aagttggatc agcatatcgg tggcaatagc ttcgatctcc ggcacgatat
    19921 aatgtggttt ttccagttca accacacggc gtaatgcatc accatcaagc atattaatga
    19981 catgggagcg atgcgcgaca tgcatggctg gtgcgtcggc atagcgatcg acggcaatca
    20041 cctctacgcc gagacgctga cactcgattg ccacttcttt acccagttca ccggagccta
    20101 ataacatcac gcgagttgct gccggacgca gcgcagtgcc taataacgtc ataacgaata
    20161 tcccctgatt aattccgcgc gcagtataaa cgaaaacgtt tgcgtgtgtc tttattgctg
    20221 atgttgattt ctcaaccgaa aagaaatata ctgtataaaa tctcagttat tatgagaggc
    20281 tgcaaatggc ggttgaagtt aaatacgtag tcattcgtga gggcgaagag aaaatgtcgt
    20341 ttaccagcaa aaaggaagcc gatgcttatg acaaaatgct tgatacggcg gatctgcttg
    20401 acacctagct gacaaattct ccagtgcaaa tggaagacga gcaacgtgaa gccctttcgc
    20461 tatggctggc agaacaaaaa gaggtgctga gcaccattct gaaaaccggc aaattaccgt
    20521 ccccacaggt cgtcggtgct gaatccgaag aggaagatgc ctcacacgcg gcctgaagca
    20581 acttgcgctc ctgacaattt gtaccatgct ttctcctgaa tcattgtgct atggagaaaa
    20641 acatgaaaaa aagaggggcg tttttagggc ggttgttggt ttctgcctgc gcatcagttt
    20701 tcgctgccaa taatgaaacc agcaagtcgg tcactttccc aaagtgtgaa ggtctggatg
    20761 ctgccggaat tgccgcgagc gtaaaacgtg attatcaaca aaatcgcgtg gcgcgctggg
    20821 cagatgatca aaaaattgtc ggtcaggccg atcccgtggc ctgggtcagt ttgcaggaca
    20881 ttcagggtaa agatgataaa tggtcagtac cgctaaccgt gcgtggtaaa agtgccgata
    20941 ttcattacca ggtctgcgtg gactgcaaag cgggaatggc ggaatatcag cggcgttaat
    21001 ggcttctgat aattcgattg accgttggcg gatgcggcgc gagtgccttg tccgtcccag
    21061 aacgcaccac aatcgtaggc ctgagaagac gcaacagcgt cgcatcgggc aatcagcatc
    21121 aaacaatgcc actcaaaacg acaaactact ttcacctaaa atacccttat cacctgatat
    21181 acagtaaata ccttacctgg cgcgaatttc tttgaataac ggcctaccgc cccgcttatt
    21241 tgcgcatctt ttgtctcgat atacattttc tgacatcctc atccgctatg ctacaggcac
    21301 taaaaatggt tattgaggag caaatatggc taactggtta aatcaactgc aatccctgct
    21361 tgggcaaagc agttcttcta cctcctcgtc tgcggatcag ggattgggca aactgttagt
    21421 gtcaggcgca ttaggcggac tggctgggct gctggtcgca aataaatcag cacgtaaact
    21481 tcttacgaaa tatggcacca acgcgttact ggttggcggc ggagcggtag cgggtacggt
    21541 gctgtggaat aaatacaaag ataaaattcg cgcggcgcat caggacgaac cgcagtttgg
    21601 cgcgcaaagt acgccgctgg atgagcgtac ggaacgtttg atccttgcgc tggtctttgc
    21661 cgctaaaagt gatggtcata ttgatgccaa tgaacgtgcg gcaatcgacc agcaattgcg
    21721 tgaagccggc gtggaagagc aggggcgtgt actcattgag caggcaatcg aacaaccgct
    21781 ggatccacaa cgcctggcta ccggggtccg caatgaagaa gaggcgctgg aaatctattt
    21841 cctgagctgc gcggctattg atattgacca ttttatggaa cgcagttacc tgaatgcact
    21901 gggtgacgcg ctgaaaatcc cccaggatgt tcgtgaaggc attgaacgcg atctcgaaca
    21961 gcaaaaacgc actttggcgg aataattccg ctctctggat agcctgaagt tgactatttc
    22021 gggctatcca gaaccgcaca gccatttgaa caggctgatt ttctctgtct gcataacatt
    22081 cgatatccgc gccattacct gtcatcatct aagcaatgac gaccctgttt cgcttgcatc
    22141 cccggtgagt tttgccaccc ttataagatg tttccaacca gaaagaacaa taacatgcta
    22201 ccaaaagccg cccgcattcc ccacgccatg acgcttcatg gcgatacgcg catcgataat
    22261 tactactggc tgcgggacga tacgcgttct cagccaaaag tcctggacta cctgcaacaa
    22321 gaaaatagtt acggtcatcg ggtgatggcc tcacaacaag ccttgcagga tcgcatctta
    22381 aaggaaatca tcgaccgcat tccgcaaaga gaagtttctg cgccctacat aaaaaatggc
    22441 taccgctatc ggcatattta tgaaccaggc tgtgaatatg ctatctacca gcgtcaatcg
    22501 gcattcagcg aagagtggga tgagtgggaa acattgctcg atgccaacaa gcgcgcagct
    22561 catagtgagt tttattcgat gggcggaatg gcgattacgc ccgataacac cattatggcg
    22621 ctggcagaag attttctttc ccgacgccag tacggcattc gttttcgtaa tctggaaaca
    22681 ggtaactggt acccggaact gctggataac gttgaaccca gctttgtctg ggcaaatgac
    22741 tcctggactt tctactatgt tcgcaagcat ccggtgacgc tgctgcctta tcaggtctgg
    22801 cgtcacgcca tcggtacgcc agcatcgcaa gatacactga tctacgaaga aaaagacgat
    22861 acctattatg tcagcctgca taaaacgacg tcgaagcact atgtcgtcat tcatctggcc
    22921 agcgccacca ccagtgaagt tcgcctgctg gacgcggaaa tggccgatgc cgagccgttt
    22981 gtttttctgc cgcgccgcaa agatcacgaa tacagccttg atcactacca gcatcgtttt
    23041 tatctgcgtt ccaatcgcca cggcaaaaac tttggcttat accgtacccg tatgcgtgat
    23101 gagcaacagt gggaagagtt aattccgcca cgcgaaaaca tcatgctgga agggtttacg
    23161 ctgtttaccg actggctggt ggttgaagag cgtcagcgcg ggttaaccag tttgcgccaa
    23221 attaaccgca agacccggga agtcattggc attgcctttg atgatccggc ctatgtgacc
    23281 tggattgcct acaatccaga acctgaaacc gcgcgattgc gttatggtta ttcttccatg
    23341 accacaccag acactttgtt tgaactggat atggataccg gtgagcgtcg tgtattaaaa
    23401 caaacagaag tgcctggttt tgatgcggcg aattaccgca gtgaacacct gtggatagtc
    23461 gcccgtgatg gcgtcgaagt tccggtttcg ctggtctatc atcgcaaaca ttttcgcaaa
    23521 ggacacaacc cgttgctggt gtatggctat ggttcttacg gcgcaagtat tgatgccgat
    23581 ttcagtttta gccgcttgag tttgttagat cgtggctttg tctacgccat tgtccatgtt
    23641 cgcggcggtg gtgagctggg gcaacaatgg tacgaagacg gtaaatttct gaagaagaaa
    23701 aatacgttta atgattatct tgatgcctgc gatgcattgt taaaactggg ctatggctct
    23761 ccttcgctct gttatgcgat gggcggaagt gcggggggca tgttgatggg cgttgcaatt
    23821 aatcaacgcc cggaattatt ccacggcgtt atcgcccagg taccgtttgt tgatgttgta
    23881 acaacgatgc ttgatgaatc aattcctctt accactggtg agtttgaaga gtggggaaat
    23941 ccgcaggatc cgcaatatta cgagtacatg aaaagctaca gcccgtatga caacgtcacc
    24001 gcacaggctt atccgcattt actggtaacg accggtttac acgattctca ggtgcaatat
    24061 tgggaaccgg caaaatgggt cgctaaattg cgcgagctga aaaccgatga ccatctttta
    24121 ttgctctgta ccgacatgga ctcaggccat ggcggtaaat ctggccgctt taaatcgtac
    24181 gaaggcgtag cgatggaata tgcttttctg gtcgcgctgg cgcagggaac attacccgct
    24241 acgcctgcgg attaagtatt ttccagataa tgtttcagtg ttaaacgcag ctccgggctc
    24301 atgctgtcca ggttattaaa taaccagcgc agatagcccg gatagcgttc ggcaacgtcg
    24361 gaaaccgctt tgccacggta tttgccaaag gtgaaggttg tcatcaacga cggacgtccg
    24421 gtgatatcgg ccatctgttc tgccgtccag ccggaggtgt tcataatatc gataagcaac
    24481 gcggcggtga tataacagtc atacaacgcg cggtgatgat gcaggcccag cggtgtctgt
    24541 acattgagct tgcgtgtttt atataacgcc atattgctgt acttgagccc tggccacaaa
    24601 cgacgggcca gtttcatagt gcaaatccac tcaccgggca tctcaggcag tacgcggcgg
    24661 tcaaagctgg cgttgtgcgc gacataccat tcactaccgt aatagtgtgg gatcacatct
    24721 tcaatccacg gtttatcggc gaccatggct tcggtgatgc gatgaatcgc catcgcttgt
    24781 ggactaatag gacgatcggg gcgcaccagg aggctcatgg ggttgacgat ttttccgtca
    24841 atgacatcaa cagaggcaat ctcaacgatc cctccctgca aaccgcaggt ttctgtatcg
    24901 ataatgcgca acatggcatg atccaggccg aaaaagccta gcgtaatgga atgatatcgc
    24961 cttgccaacc ctgtgatgaa cgctgaccga ctaacaataa tgaaccacga tcggcgcgaa
    25021 cgatgagacg accatattca tcccatagtg cactgttacc acgggcattt gccatcagca
    25081 cggcaatcga gtactgatgt gaaaaaaatt gtaagcgacg ggccgacgcc agcaggtcgg
    25141 gttctcccag acattgccct gtcgtaaaca gtgaacaggt cgggtccata tccatacctt
    25201 gcggttgttc atcgaccacc gtgatggttc tggaacgacg gcccagacat gcaccatgac
    25261 tctgatggta gttcccaggc gtttttcgcc acggtgcgaa caccgcaatt ccacgaataa
    25321 agcgatcgtt atattcaacg ggaaggccgg caatgatggt cattcgctag gtcgttgcgg
    25381 cataacaaag cgggtctaac agtgaaagat cgggtggggc aggaagggca cgtcgcgaat
    25441 aatcacaccc cagtaaagaa agcgatggaa aaactaacag ctgacattgt tgcctggcgg
    25501 cggccctcac aaactccaga tgatgagcta cctgttcggt gagtgaggtt ttacgaggct
    25561 catattgcgc tgctgcaact ttccagaacg acataatgac ttccttttca aaatgactgc
    25621 aaattatggg ggcggtttag aaaatactca taatttagca ggcgttatgt aagaaagtgt
    25681 aactctgtat aagttttatt taagtttggg ctcgtaaggt aaacgtgaca gattgaccga
    25741 agccaaacgg tgggcaataa gccgctcgcg aaaccagctg cgcaaatgtt ctggctgttc
    25801 acgttcaacc gcttcagcga tcaccggcat attgtagcgt tctttaaaag ccaccccggc
    25861 cgccgccaaa tcgacattca ctttatccat ttctgtttga tccagtttag ccagattctt
    25921 cagcattctt aatctcctca tcattcgcgg gcaaaagtta ctaagagtga gcctggatgg
    25981 caagccggaa ttgatgatcc tcgactcaga cattagacaa ggttatgctc tgtaaaaaag
    26041 ttattacaaa aaggaataga taatatggct tcaactgcac gctcccttcg ctacgcgctg
    26101 gcaatcctga ccacttcgtt ggtgacacct tccgtctggg cacatgcgca tttaacgcat
    26161 cagtatcctg cggcaaacgc gcaagtgaca gctgccccgc aggcaatcac tttaaacttc
    26221 tcggaaggcg ttgaaacagg attcagtggt gcaaaaatca cggggccaaa aaacgaaaat
    26281 attaaaacat taccagcgaa gcggaatgag caggaccaaa agcaactgat tgttccactg
    26341 gccgattcgc tgaaacccgg aacctacacc gttgactggc atgttgtttc ggtggacggg
    26401 cataaaacga aaggacacta cacctttagc gtgaaataaa tcatgctggc gtttacctgg
    26461 atcgcgctgc gatttatcca tttcacctcg ttgatgctgg tctttggctt tgcaatgtat
    26521 ggtgcctggc tggccccctt aatgattcgt cgcttgctga cgaagcgttc tctgcgttta
    26581 caacagcacg ctgccgtctg gagtttgatc agtgccactg caatgctcgc cgttcagggc
    26641 gggttgatgg gaacgggatg gacagatgta ttttcgccaa acatttggca ggctgttttg
    26701 caaacgcagt ttggtggcgt ctggttatgg caaattgttc tcgccctcgt aacgttgatc
    26761 gtcgccctta tgcaaccgcg gaatatgcca cgtttgctgt ttatgctcac taccgcgcaa
    26821 tttattctgc tggcgggagt ggggtatgcg acgctgaatg aaggggtaac agcgaaaatc
    26881 catcagacta atcacgcgat tcacctgatt tgtgcggcgg cgtggtttgg gggattactg
    26941 ccggtgctct ggtgtatgca actcatcaaa ggccgttggc gacactaggc tattcaggcg
    27001 ctgatgcgtt tttcctggtg cgggcatttt gcggtgatcg gcgtactggc aagcggcgtg
    27061 cttaatgcat tgttgattac cggatttccc cccacgctta cgacttactg gggccaactc
    27121 ttattgctca aagccatcct ggtgatgatt atggtggtca ttgcgctggc taatcgttat
    27181 gttcttgtac cgcgcatgag gcaggatgaa gatcgtgcgg caccctggtt tgtgtggatg
    27241 actaaactcg aatgggcgat aggtgcggta gtgctggtga tcatcagcct gcttgcgact
    27301 ctcgaacctt tttgatggat tacgaaaatg atgaaaaaaa gtattctggc gtttctgtta
    27361 ctcaccagtt ctgcagcggc gctggcggct ccacaggtga taaccgtgag tcgttttgaa
    27421 gtgggtaaag acaaatgggc gtttaatcgc gaagaggtaa tgctgacttg ccgaccgggt
    27481 aatgctttgt atgtcatcaa cccaagtacc ctcgtgcagt atcctttaaa cgatatcgca
    27541 caaaaggaag ttgccagtgg gaagaccaat gcccaaccca tttcggtgat tcagattgat
    27601 gatcctaaca atcccggcga aaaaatgagt ctggcaccgt ttatagaacg agctgaaaaa
    27661 ctctgttaat tacctaaaat agccttttga tttccaataa aaaaaccgcc tcagttcttt
    27721 caccagaacg ggcggttttt aacatttaag ctgatgagca ccgcgctttt tattgaccat
    27781 tttgcacgca aactggaaaa cctggcgtcg tcatctattc ttaaagggca aggcaactaa
    27841 gcctgcatta atgccaactt ttagcgcacg gctctctccc aagagccatt tccctggacc
    27901 gaatacagga atcgtattcg gtcttttttt aattgtattt aaaatcaatt agttgcaaac
    27961 gtctccccga aattccccga aatttactcg aatttctgta ttccggtctt ttttggttat
    28021 atcacaacca aaatacattt aacaatccat ttacgttaaa atcagagcag taagtacgtt
    28081 ttttctctct catcaagata catttttgtt gtcttctccg atgtgtggcc aagtagacgc
    28141 tgagcaaatt cttctccaca tatttctttg tacaatcgtc cagccaggct tctgatttcg
    28201 tgaaaagttg gtgggttttc actgaacagt gatacctgtt aattttctgg ctgcgacaaa
    28261 tttctttgtc aggccgtccg ggtgaatgct gccgtcaggg ctgttttttc taatcccggc
    28321 actgattaga taatcccccc tacttaccat gcggcactgt tcaactactg caccaagtcg
    28381 taaaccagcg acaggaaggc tgagtgacag ggggatagca atcatcattc ctgtcttaat
    28441 ttgcctgatg tggagacgat catcataaat atcactgaac cgcatattcg ttatgtcttc
    28501 gcgacgttgt cctgttacaa gggctaagtc catagctaat gggaaccatg ccggaagttg
    28561 atctgctgcc tccctgatgc agttgtatgt ctttagtttc agccgttctc ttgtaactac
    28621 tattttcggt gctcttgttg gcgttactgg attttgagat atacgtcctt caacaatggc
    28681 ttcgcggaac atatcagata acacagaacg cattgatcct gccatcgtgt ttttccctcc
    28741 ttcaatctac aaatcaagaa actcggcgat atggcgagtg gttatttctg tcagtaaaac
    28801 ccctcctaat ttttctttta ttgtctccag ttgatttacc cgagttttat aagtatttct
    28861 ggacgctttt ctccttataa gaatcgtttt gtaacgttca atccagcctg ccatagtaaa
    28921 tgagtcgacc cctttaagct tttcaattag ggcagcagga gagtagtttt tgtatatata
    28981 atgatttgct tcaattgcct gcgctactgc atctcttctt gaaattttac ctagtgtaaa
    29041 ttcttctttc gtcagagggt tgcgccagta atatgctttg tctctccttc gatatgttaa
    29101 gtttctaggc aaattgggat cgtatttttt ccgctgcatg ttttaacttc tccattaacg
    29161 gactgtctct cccttgtcgc ccattaggct gatggtgtgt tatatcggta tcaaccttat
    29221 ttgggttgat atagaaagcc tccggaacca ccctgtaact cctcccgtgt agttcaggtg
    29281 caggataaat gtttccattc cttgcccatc gtctcagcgt tgatattgat ggtgggttat
    29341 ccggatatct gagttttccc cacgttttga gtgtcacaag attcattgcc atacctctca
    29401 cgatatgacc gccagtaaat atacagaata ctggcgggtg tggttgattt ttaata
//
LOCUS       DAJTRC010000047        29043 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.047, whole genome shotgun sequence.
ACCESSION   DAJTRC010000047 DAJTRC010000000
VERSION     DAJTRC010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 29043)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29043)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29043
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.047"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            109..1140
                     /gene="mdtN"
                     /locus_tag="OO357_002198"
     CDS             109..1140
                     /gene="mdtN"
                     /locus_tag="OO357_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709857.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter periplasmic adaptor
                     subunit MdtN"
                     /protein_id="HCR5887133.1"
                     /translation="MESTPKKAPRSKFPALLVVALALVALVFVIWRVDSAPSTNDAYA
                     SADTIDVVPEVSGRIVELAVTDNQAVKQGDLLFRIDPRPYEANLAKAEASLAALDKQI
                     MLTQRSVDAQQFGADSVNATVEKARAAAKQATDTLRRTEPLLKEGFVSAEDVDRARTA
                     QRAAEADLNAVLLQAQSAASAVSGVDALVAQRAAVEADIALTKLHLEMTTVRAPFDGR
                     IISLKTSVGQFASAMRPIFTLIDTRHWYVIANFRETDLKNIRSGTPATIRLMSDSGKT
                     FEGKVDSIGYGVLPDDGGLVLGGLPKVSRSINWVRVAQRFPVKIMVDKPDPEMFRIGA
                     SAVANLEPQ"
     gene            1140..3182
                     /gene="mdtO"
                     /locus_tag="OO357_002199"
     CDS             1140..3182
                     /gene="mdtO"
                     /locus_tag="OO357_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter permease subunit
                     MdtO"
                     /protein_id="HCR5887134.1"
                     /translation="MSALNSLPLPVVRLLAFFHEELSERRPGRVPQIVQLWVGCLLVI
                     LISMTFEIPFVALSLAVLFYGIQSNAFYTKFVAILFVVATVLEIGSLFLIYKWSYGEP
                     LIRLIIAGPILMGCMFLMRTHRLGLVFFAVAIYGQTFPAMLDYPEVVVRLTLWCIVVG
                     LYPTLLMTLIGVLWFPNRAITQMHQALNDRLDDAISHLTDSLAPLPETRIEREALALQ
                     KLNVFCLADDANWRTQSAWWQSCVATVTYIYSTLNRYDPTSFADSQAIIEFRQKLASE
                     INKLQHAVAEGQCWQSDWRLSESEAVAARECNLENICQTLLQLGQMNPNTPPTPAAKP
                     PSMVADAFTNPDYIRYAVKTLLACLICYTFYSGVDWEGIHTCMLTCVIVANPNVGSSY
                     QKMVLRFGGAFCGAILALLFTLLVMPWLDNIVELLFVLAPIFLLGAWIATSSERSSYI
                     GTQMVVTFALATLENVFGPVYDLVEIRDRALGIIIGTVVSAMIYTFVWPESEARTLPQ
                     KLAGALGMLSKVMRIPRQQEVTALRTYLQIRIGLHAAFNACEEMCQRVALERQLDSEE
                     RALLIERSQTVIRQGRDILHAWDATWNSAQALDNALQPDRAGQFADALEKYAAGLATA
                     LSRSPQITLEETPTSQAILPTLLKQEQHVCQLFARLPDWTAPALTPATEQAQGATQ"
     gene            3179..4645
                     /gene="mdtP"
                     /locus_tag="OO357_002200"
     CDS             3179..4645
                     /gene="mdtP"
                     /locus_tag="OO357_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter outer membrane
                     subunit MdtP"
                     /protein_id="HCR5887135.1"
                     /translation="MINRQLSRLLLCSILGSTTLISGCALVRKDSAPHQQLKPEQIKL
                     ADDIHLASSGWPQAQWWKQLNDPQLDALIQRTLSGSHTLAEAKLREEKAQSQADLLDA
                     GSQLQVAALGMLNRQRVSANGFLSPYAMDAPALGMDGPYYTEATVGLFAGLDLDLWGV
                     HRSAVAAAIGAHNAALAETAAVELSLTTGVAQLYYSMQASYQMLDLLEQTRDVIDYAV
                     KAHQSKVAHGLEAQVPFHGARAQILAVDKQIAAVKGQITETRESLRALIGAGASDMPE
                     IKPVALPRVQTGIPATLSYELLARRPDLQAMRWYVQASLDQVDSARALFYPSFDIKAF
                     FGLDAIHLDTLFKKTSRQFNFIPGLKLPLFDGGRLNANLEGTRAASNMMIERYNQSVL
                     NAVRDVAVNGTRLQTLNDEREMQAEHVEATRFTQRAAEAAYQRGLTSRLQATEARLPV
                     LAEEMSLLMLDSRRVIQSIQLMKSLGGGYQAAPVVEKK"
     gene            4843..5082
                     /locus_tag="OO357_002201"
     CDS             4843..5082
                     /locus_tag="OO357_002201"
                     /inference="COORDINATES: protein motif:HMM:NF016750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887136.1"
                     /translation="MKKVVTVCPYCASGCKINLVVDNGKIVRAEAAQGKTNQGTLCLK
                     GYYGWDFINDTQILTPRLKTPMIRRQRGGKLEPVS"
     gene            5092..5262
                     /locus_tag="OO357_002202"
     CDS             5092..5262
                     /locus_tag="OO357_002202"
                     /inference="COORDINATES: protein motif:HMM:NF012602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5887137.1"
                     /translation="MNYVAERLSAIKEKYGPDAIQTTGSSRGTGNETNYVMQKFARAV
                     IGTNNVDCCARV"
     gene            5311..6723
                     /gene="fdhF"
                     /locus_tag="OO357_002203"
     CDS             5311..6723
                     /gene="fdhF"
                     /locus_tag="OO357_002203"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HCR5887138.1"
                     /translation="MSNAINEIDNTDLVFVFGYNPADSHPIVANHVINAKRNGAKIIV
                     CDPRKIETARIADMHIALKNGSNIALLNAMGHVIIEENLYDKAFVASRTEGFEEYRKI
                     VEGYTPESVEDITGVSASEIRQAARMYAQAKSAAILWGMGVTQFYQGVETVRSLTSLA
                     MLTSNLGKPHAGVNPVRGQNNVQGACDMGALPDTYPGYQYVKDPANREKFAKAWGVES
                     LPAHTGYRISELPHRAAHGEVRAAYIMGEDPLQTDAELSAVRKAFEDLELVIVQDIFI
                     TKTASAADVILPSTSWGEHEGVFTAADRGFQRFFKAVEPKWDLKTDWQIISEIATRMG
                     YPMHYNNTQEIWDELRHLCPDFYGATYEKMGELGFIQWPCRDTSDADQGTSYLFKEKF
                     DTPNGLAQFFTCDWVAPIDKLTDEYPMVLSTVREVGHYSCRSMTGNCAALAALADEPG
                     YAQINTEDAKRLGIEDEALV"
     gene            6724..6990
                     /locus_tag="OO357_002204"
     CDS             6724..6990
                     /locus_tag="OO357_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000556186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HCR5887139.1"
                     /translation="MHSRKGKIITRAQVSDRPNKGAIYMTYQWWIGACNELVTESLSP
                     ITKTPEYKYCAVRVEPISDQRAAEQYVIDEYNKLKTRLREAALA"
     gene            7084..7773
                     /locus_tag="OO357_002205"
     CDS             7084..7773
                     /locus_tag="OO357_002205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sel1 repeat family protein"
                     /protein_id="HCR5887140.1"
                     /translation="MKKIIALMLFLTFFAHANDSEPGSQYLKAAEAGDRRAQYFLADS
                     WFSSGDLSKAEYWAQKAADSGDADACALLAQIKITNPVSLDYPQAKVLAEKAAQAGSK
                     EGEVTLAHILVNTQAGKPDYPKAISLLENASEDLENDSAVDAQMLLGLIYANGVGIKA
                     DDDKATWYFKRSSAISRTGYSEYWAGMMFLNGEEGFIEKNKQKALHWLNLSCMEGFDT
                     GCEEFEKLTNG"
     gene            complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO357_002206"
     CDS             complement(7851..9164)
                     /gene="gltP"
                     /locus_tag="OO357_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate/aspartate:proton symporter GltP"
                     /protein_id="HCR5887141.1"
                     /translation="MKNIKFSLAWQILFAMVLGILLGSYLHYHSDSRDWLVVNLLSPA
                     GDIFIHLIKMIVVPIVISTLVVGIAGVGDAKQLGRIGAKTIIYFEVITTVAIILGITL
                     ANVFQPGAGVDMSQLATVDISKYQSTTEAVQSSSHGIMGTILSLVPTNIVASMAKGEM
                     LPIIFFSVLFGLGLSSLPATHREPLVTVFRSISETMFKVTHMVMRYAPVGVFALIAVT
                     VANFGFSSLWPLAKLVLLVHFAILFFALVVLGIVARLCGLSVWILIRILKDELILAYS
                     TASSESVLPRIIEKMEAYGAPASITSFVVPTGYSFNLDGSTLYQSIAAIFIAQLYGID
                     LSIWQEIILVLTLMVTSKGIAGVAGVSFVVLLATLGSVGIPLEGLAFIAGVDRILDMA
                     RTALNVVGNALAVLVIAKWEHKFDRKKALAYEREVLGKFDKTADQ"
     gene            complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO357_002207"
     CDS             complement(9506..10102)
                     /gene="nrfG"
                     /locus_tag="OO357_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfG"
                     /protein_id="HCR5887142.1"
                     /translation="MKQPQIPVKMLTTLTILMVFLCVGSYLLSPKWQAVRAEYQRQRD
                     PLHQFASQQTPEAQLQALQDKIRANPQNSEQWALLGEYYLWQNDYSNSLLAYRQALQL
                     RGENAELYAALATVLYYQASQHMTAQTRAMIDKALALDSNEITALMLLASDAFMQANY
                     AQAIELWQKVMDLNSPRINRTQLVESINMAKLLQRRSD"
     gene            complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO357_002208"
     CDS             complement(10099..10482)
                     /gene="nrfF"
                     /locus_tag="OO357_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase NrfEFG subunit NrfF"
                     /protein_id="HCR5887143.1"
                     /translation="MNKGLLTLLLLFTCFARAQVVDTWQFANPQQQQQALNIASQLRC
                     PQCQNQNLLESNAPVAVSMRHQVYSMVSEGKNEVEIIGWMTERYGDFVRYNPPLTGQT
                     LVLWALPVVLLLLMALILWRVRAKR"
     gene            complement(10475..12193)
                     /locus_tag="OO357_002209"
     CDS             complement(10475..12193)
                     /locus_tag="OO357_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HCR5887144.1"
                     /translation="MDVFLPEVGFLALLLSLGVNVLTPLTAFAGVRLRWPAMMRLTCI
                     GILAQFALLLLAFGVLTYCFLISDFSVIYVAQHSYSLLSWELKLAAVWGGHEGSLLLW
                     VLLLSAWSALFAWHYRQQSDPLFPLTLAVLSLMLAALLLFVVLWSDPFVRIFPPAIEG
                     RDLNPMLQHPGLIFHPPLLYLGYGGLMVAASVALASLLRGEFDAASARICWRWALPGW
                     SALTAGIILGSWWAYCELGWGGWWFWDPVENASLLPWLSATALLHSLSLTRQRGIFRH
                     WSLLLAIVTLMLSLLGTLIVRSGILVSVHAFALDNVRAVPLFSLFALISLASLALYGW
                     RARDGGPAVRFSGLSREMLILATLLLFCAVLLIVLVGTLYPMIYGLLGWGRLSVGAPY
                     FNRATLPFGLLMLVVIVLATFVSGKRVQLPALVAHAGVLLFAAGIVVSSVSRQEISLN
                     LQPGQQVTLAGYTFRFERLDLQARGNYTSEKAIVALFDHQQRIGELTPERRFYEARRQ
                     QMMEPSIRWNGIHDWYAVMGEKTRADRYAFRLYVQSGVRWIWGGGLLMIAGALLSGWR
                     GKKRDE"
     gene            complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO357_002210"
     CDS             complement(12213..13169)
                     /gene="nrfD"
                     /locus_tag="OO357_002210"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase subunit NrfD"
                     /protein_id="HCR5887145.1"
                     /translation="MTQTSAFHFESLVWDWPIAIYLFLIGISAGLVTLAVLLRRFYPQ
                     AGGADSTLLRTTLIVGPGAVILGLLILVFHLTRPWTFWKLMFHYSFTSVMSMGVMLFQ
                     LYMVVLVLWLAKIFEHDLLALQQRWLPKLGIVQKVLSLLTPVHRGLETLMLVLAVLLG
                     AYTGFLLSALKSYPFLNNPSLPVLLLFSGISSGAAVALIAMAIRQRSNPHSTEAHFVH
                     RMEIPVVWGEIFLLVAFFVGLALGDDGKVRALVAALGGGFWTWWFWLGVAGLGLIVPM
                     LLKPWVNRSSGIPAVLAACGASLVGVLMLRFFILYAGQLTVA"
     gene            complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO357_002211"
     CDS             complement(13166..13837)
                     /gene="nrfC"
                     /locus_tag="OO357_002211"
                     /EC_number="1.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase Fe-S protein"
                     /protein_id="HCR5887146.1"
                     /translation="MTWSRRQFLTGVGVLAAVSGTAGRVVAKTLNINGVRYGMVHDES
                     LCIGCTACMDACREVNKVPEGVSRLTIIRSEPQGEFPDVKYRFFRKSCQHCDHAPCVD
                     VCPTGASFRDAASGIVDVNPDLCVGCQYCIAACPYRVRFIHPVTKTADKCDFCRKTNL
                     QAGKLPACVEACPTKALTFGNLDDPNSEISQLLRQKPTYRYKLALGTKPKLYRVPFKY
                     GEVSQ"
     gene            complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO357_002212"
     CDS             complement(13834..14400)
                     /gene="nrfB"
                     /locus_tag="OO357_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709847.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c nitrite reductase pentaheme
                     subunit"
                     /protein_id="HCR5887147.1"
                     /translation="MSVLRSLLTAGVLASGLLWSLNGITATPAVQASGDRYEVTQQRN
                     PDAACLDCHKPDTEGMHGKHASVINPNNKLPVTCTNCHGQASPQHREGVKDVMRFNEP
                     MYKVGEQNSVCMSCHLPEQLQKAFWPHDVHVTKVACASCHSLHPQQDTMQTLSDKGRI
                     KICVDCHSDQRTNPNFNPASVPLLKEQP"
     gene            complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO357_002213"
     CDS             complement(14445..15881)
                     /gene="nrfA"
                     /locus_tag="OO357_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-forming nitrite reductase cytochrome
                     c552 subunit"
                     /protein_id="HCR5887148.1"
                     /translation="MTRIKINARRIFSLLIPFFFFTSVHAEQTAAPAKPVTVEAKNET
                     FAPQHHDQYLSWKATSEQSERVDALAEDPRLVILWAGYPFSRDYNKPRGHAFAVTDVR
                     ETLRTGAPKNAEDGPLPMACWSCKSPDVARLIQKDGEDGYFHGKWARGGPEIVNNLGC
                     ADCHNTASPEFAKGKPELTLSRPYAARAMEAIGKPFEKAGRFDQQSMVCGQCHVEYYF
                     DGKNKAVKFPWDDGMKVENMEQYYDKIAFSDWTNSLSKTPMLKAQHPEYETWTAGIHG
                     KNNVTCIDCHMPKVQNAEGKLYTDHKIGNPFDNFAQTCANCHTQDKAALQKVVAERKQ
                     SINDLKIKVEDQLVHAHFEAKAALDAGATEAEMKPIQDDIRHAQWRWDLAIASHGIHM
                     HAPEEGLRMLGTAMDKAADARTKLARLLATKGITHEIQIPDISTKEKAQQAIGLNMEQ
                     IKAEKQDFIKTVIPQWEEQARKNGLLSQ"
     gene            16274..18232
                     /gene="acs"
                     /locus_tag="OO357_002214"
                     /pseudo
     CDS             16274..18232
                     /gene="acs"
                     /locus_tag="OO357_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418493.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase"
     gene            18432..18746
                     /locus_tag="OO357_002215"
     CDS             18432..18746
                     /locus_tag="OO357_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF485 domain-containing protein"
                     /protein_id="HCR5887149.1"
                     /translation="MNGTIYQRIEDNAHFRELVEKRQRFATILSIIMLAVYIGFILLI
                     AFAPGWLGTPLNPNTSVTRGIPIGVGVIVISFVLTGIYIWRANGEFDRLNNEVLHEVQ
                     AS"
     gene            18743..20392
                     /gene="actP"
                     /locus_tag="OO357_002216"
     CDS             18743..20392
                     /gene="actP"
                     /locus_tag="OO357_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation/acetate symporter ActP"
                     /protein_id="HCR5887150.1"
                     /translation="MKRVLTALAATLPFAANAADAISGAVERQPTNWQAIIMFLIFVV
                     FTLGITYWASKRVRSRSDYYTAGGNITGFQNGLAIAGDYMSAASFLGISALVFTSGYD
                     GLIYSLGFLVGWPIILFLIAERLRNLGRYTSADVASYRLKQGPIRILSACGSLVVVAL
                     YLIAQMVGAGKLIELLFGLNYHIAVVLVGVLMMMYVLFGGMLATTWVQIIKAVLLLFG
                     ASFMAFMVMKHVGFSFNNLFSEAMAVHPKGVDIMKPGGLVKDPISALSLGLGLMFGTA
                     GLPHILMRFFTVSDAREARKSVFYATGFMGYFYILTFIIGFGAIMLVGANPEYKDAAG
                     HLIGGNNMAAVHLANAVGGNLFLGFISAVAFATILAVVADLTLAGASAVSHDLYANVF
                     KKGATEREELRVSKITVLILGVIAIILGVLFENQNIAFMVGLAFAIAASCNFPIILLS
                     MYWSKLTTRGAMLGGWLGLITAVVLMILGPTIWVQILGHEKAIFPYEYPALFSISVAF
                     LGIWFFSATDNSAEGARERELFRAQFIRSQTGFGVEQGRAH"
     gene            20570..21862
                     /locus_tag="OO357_002217"
     CDS             20570..21862
                     /locus_tag="OO357_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HCR5887151.1"
                     /translation="MRYNGLNNMFFPLCQINDNHSITSSSHTKKTKSDNYSKHHKNTL
                     IDNKALSLFKIDDHEKVIGLIQKMKRIYDSLPSGKITKETDRKIHKYFIDIALYANNK
                     CDDRITRRVYLSKEKDVSIKVVYFINNVAVHNNTIEIPQIVNGGYDFSHLSLKGIVIK
                     DEDLSNSNFAGCRLQNAIFQDCNMYKTNFYYAIMEKILFDNCILDDSNFAQIKMADGT
                     LNACSAMHVQFYNAAMNRANIKNTFLDYSNFYMAYMAEVNLYKVIAPYVNLFKADLSF
                     SKLDLINFEHADLSRVNLNKAILQNINLIDSKLFCTWLTNTFLEMVICTGSNMANVNF
                     NNANLSNCHFNCSILTKACMFNTRLYRVNFDEASVQGMGISILRGEENIPIDSDTLVT
                     LQKFFEEDCTSHTGMSQTEDNINAVAMKITADIMQHAD"
     gene            complement(22016..23665)
                     /locus_tag="OO357_002218"
     CDS             complement(22016..23665)
                     /locus_tag="OO357_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter"
                     /protein_id="HCR5887152.1"
                     /translation="MEIFFTILIMTLVVSLSGVVTRVMPFQIPLPLMQIAIGALLAWP
                     TFGLHVEFDPELFLVLFIPPLLFADGWKTPTREFLEHGREIFGLALALVVVTVVGIGF
                     LIYWVVPGIPLIPAFALAAVLSPTDAVALSGIVGEGRIPKKIMGILQGEALMNDASGL
                     VSLKFAVAVAMGTMIFTVGGATVEFMKVAIGGILAGFVVSWLYGRSLRFLSRWGGDEP
                     ATQIVLLFLLPFASYLIAEHIGVSGILAAVAAGMTITRSGVMRRAPLAMRLRANSTWA
                     MLEFVFNGMVFLLLGLQLPGILETSLMAAEIDPNVEIWMLFTDIILIYAALMLVRFGW
                     LWTMKKFSNRFLKKKPMEFGSWTTREILIASFAGVRGAITLAGVLSIPLLLPDGNVFP
                     ARYELVFLAAGVILFSLFVGVVMLPILLQHIEVADHSQQLKEERIARAATAEVAIVAI
                     QKMEERLAADTEENIDNQLLTEVSSRVIGNLRRRADGRNDVESSVQEENLERRFRLAA
                     LRSERAELYHLRATREISNETLQKLLHDLDLLEALLIEENQ"
     gene            complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO357_002219"
                     /gene_synonym="yjcD"
     CDS             complement(23816..25165)
                     /gene="ghxP"
                     /locus_tag="OO357_002219"
                     /gene_synonym="yjcD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanine/hypoxanthine transporter GhxP"
                     /protein_id="HCR5887153.1"
                     /translation="MSTPSARTGGSLDAWFKISQRGSTVRQEVVAGLTTFLAMVYSVI
                     VVPGMLGKAGFPPAAVFVATCLVAGLGSIVMGLWANLPLAIGCAISLTAFTAFSLVLG
                     QHISVPVALGAVFLMGVLFTVISATGIRSWILRNLPHGVAHGTGIGIGLFLLLIAANG
                     VGLVIKNPLDGLPVALGDFATFPVIMSLAGLAVIIGLEKLKVPGGILLTIIGISIVGL
                     IFDPNVHFSGVFAMPSLSDENGNSLIGSLDIMGALNPVVLPSVLALVMTAVFDATGTI
                     RAVAGQANLLDKDGQIIDGGKALTTDSMSSVFSGLVGAAPAAVYIESAAGTAAGGKTG
                     LTAITVGVLFLLILFLSPLSYLVPGYATAPALMYVGLLMLSNVAKIDFADFVDAMAGL
                     VTAVFIVLTCNIVTGIMIGFATLVIGRLVSGEWRKLNIGTVVIAVALVTFYAGGWAI"
     gene            complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO357_002220"
     CDS             complement(25871..26335)
                     /gene="soxR"
                     /locus_tag="OO357_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HCR5887154.1"
                     /translation="MEKKLPRIKALLTPGEVAKRSGVAVSALHFYESKGLITSIRNSG
                     NQRRYKRDVLRYVAIIKIAQRIGIPLATIGEAFGVLPEGHTLSAKEWKQLSSQWREEL
                     DRRIHTLVALRDELDGCIGCGCLSRSDCPLRNPGDRLGEEGTGARLLEDEQN"
     gene            26421..26744
                     /gene="soxS"
                     /locus_tag="OO357_002221"
     CDS             26421..26744
                     /gene="soxS"
                     /locus_tag="OO357_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide response transcriptional regulator
                     SoxS"
                     /protein_id="HCR5887155.1"
                     /translation="MSHQKIIQDLIAWIDVHIDQPLNIDVVAKKSGYSKWYLQRMFRT
                     VTHQTLGDYIRQRRLLLAAVELRTTERPIFDIAMDLGYVSQQTFSRVFRRQFDRTPSD
                     YRHRL"
     gene            complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO357_002222"
                     /pseudo
     CDS             complement(26747..28324)
                     /gene="pdeC"
                     /locus_tag="OO357_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418485.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="c-di-GMP phosphodiesterase PdeC"
     gene            28753..29034
                     /locus_tag="OO357_002223"
     CDS             28753..29034
                     /locus_tag="OO357_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcB family protein"
                     /protein_id="HCR5887156.1"
                     /translation="MATLTTGVVLLRWQLLSAVMMFLASTLNIRFRRSDYVGLAVISS
                     GLGVVSACWFAMGLLGITMADITAIWHNIESVMIEEMNQTPPQWPMILT"
BASE COUNT         7284 a         7776 c         7352 g         6631 t
ORIGIN      
        1 cgggcaaata tcaatctggc atttgtcgga attatttata ccgccctttt tgctctctac
       61 gccatgctgt tctggctggc atttttctaa tctatttgag acgcgctcat ggaaagtacg
      121 ccgaaaaaag cacctcgcag taaattccct gctctgttag tggttgcgtt ggcgctggtt
      181 gcccttgttt tcgttatctg gcgcgtagac agtgcgccat caactaatga cgcttacgcg
      241 tcagcagata ccattgatgt ggtgccggaa gtcagcggtc gcattgtaga actggcggtc
      301 accgacaacc aggcggtgaa acagggcgat ttgctgttcc gcatcgaccc gcgcccgtac
      361 gaagccaacc tggcgaaagc cgaagcctcc ctcgcggcgc tggataagca aattatgctc
      421 acccagcgta gcgttgacgc ccaacagttt ggtgccgact cggttaatgc cacggtagaa
      481 aaagcccgtg ccgccgcgaa acaggccaca gatacattac gccgcaccga gccattactg
      541 aaagagggtt ttgtctcagc agaagacgtt gaccgtgcaa gaacggcgca gcgcgccgca
      601 gaagcggatc ttaatgccgt attgttacag gcgcagtcag ccgccagcgc cgtcagcggc
      661 gtcgatgcgc tggtggcgca acgtgcggcg gtcgaagcgg atattgccct gaccaaacta
      721 catctggaaa tgacgaccgt tcgcgcgcca tttgatggcc ggatcatttc cctcaaaacc
      781 tccgtcgggc aatttgcttc tgccatgcgc cctattttta ccctcatcga cactcgtcac
      841 tggtacgtga tcgccaactt ccgcgaaacc gatctgaaaa atattcgctc aggtacaccc
      901 gcaacgattc gcctgatgag tgacagcggc aaaaccttcg agggtaaagt ggattcgatt
      961 ggctacggcg tgctaccgga tgacggcggc ctggtgctgg gcggcctgcc aaaagtgtct
     1021 cgttctatta actgggtccg cgttgcccag cgttttccgg tcaaaatcat ggtcgataaa
     1081 cctgacccgg aaatgttccg catcggcgct tcggcagtcg ctaatcttga gccgcaataa
     1141 tgagcgcgct caactccctg ccattaccgg tggtcaggct gctggcgttc tttcatgaag
     1201 agttaagcga gcggcgaccc ggccgcgtgc cgcagatcgt gcaactctgg gtaggctgcc
     1261 tgctggtgat tctgatctcg atgacttttg agatcccttt tgtggcgtta tcgctggcag
     1321 tgctgtttta cggtattcag tcgaacgcgt tttacaccaa atttgtggcg atcttgtttg
     1381 tggttgccac ggtgctggag atcggcagcc tgtttttgat ctacaaatgg tcatacggcg
     1441 aaccgttgat ccgattgatc atcgccgggc cgatcctgat gggctgcatg tttttgatgc
     1501 gcacccatcg gctgggactg gtctttttcg ctgtcgccat ttacgggcaa accttccccg
     1561 ccatgctcga ctatccggaa gtagtcgtgc gcttaacgct gtggtgtatc gttgttggcc
     1621 tctatccaac cttgctgatg acgttaatcg gcgtgctgtg gtttcccaat cgtgccatta
     1681 cgcaaatgca tcaagcgctt aatgatcggc ttgatgatgc cattagccac ctgacggaca
     1741 gcctcgcacc gctacccgaa acgcggattg aaagagaggc gctggcgctg caaaaactga
     1801 atgtcttttg tctcgcggac gatgccaact ggcgaactca aagcgcatgg tggcaaagct
     1861 gcgtggcaac ggtaacctac atttactcga cgctgaatcg ctacgatccc acctcttttg
     1921 ctgattctca ggcaattatt gaatttcggc aaaaattagc ctcagaaatc aacaagctgc
     1981 agcatgccgt tgctgaaggt cagtgctggc aaagcgactg gcggctcagt gaaagtgaag
     2041 cggtggcggc acgggaatgt aacctggaga atatctgcca gacgttgtta caactgggcc
     2101 agatgaaccc gaatacgccg ccaacgcccg ccgccaaacc gccatcaatg gtcgccgatg
     2161 cttttaccaa tccagactat atacgctacg cggtaaaaac attgctcgcc tgtttgatct
     2221 gttacacctt ttacagcggc gtggactggg aaggcattca cacctgtatg ctgacctgcg
     2281 tgatcgtcgc taatccaaat gtcggttcgt cgtaccagaa gatggtgctg cgttttggcg
     2341 gggccttttg cggcgcgatt ctggcgctgt tattcacgct actggtcatg ccctggctgg
     2401 acaatattgt cgaattgctg tttgtgctgg caccgatttt cctgttgggc gcatggattg
     2461 ccaccagctc tgaacgctct tcttatatcg gcacacagat ggtggtcacc ttcgcgctcg
     2521 ccacgctcga aaacgttttt ggcccagtgt acgacctggt ggaaattcgc gatcgcgccc
     2581 tgggtatcat cattggtacc gtggtgtccg cgatgattta cacctttgtc tggcctgaaa
     2641 gtgaagcgcg cacgctgccg caaaaactgg ctggcgcgct gggtatgtta agtaaagtaa
     2701 tgcggatccc acgccagcag gaagtcacgg ctctgcgcac ttatctgcaa attcgtatcg
     2761 gtctgcatgc ggcgtttaat gcctgtgaag agatgtgcca acgcgtggcg ctggagcgtc
     2821 aactggacag cgaagaacgc gccttactga ttgaacgttc gcaaacggtt attcgtcagg
     2881 gccgcgatat ccttcacgcc tgggatgcca cctggaattc ggcgcaggcg ctggataacg
     2941 cactacagcc ggacagagca ggtcagtttg ccgacgccct ggagaaatac gctgccggtc
     3001 tggcaaccgc actcagccgt tctcctcaaa taacgcttga agagacaccc acctcgcagg
     3061 ccatcctgcc caccttatta aaacaggagc aacacgtctg ccagcttttc gcccgcttgc
     3121 cagactggac agccccggca ttaacgcccg ccacggaaca ggcacaagga gccacgcaat
     3181 gatcaatcgt caactttcac gtctgctgtt gtgcagcatt ctcggcagca cgacgctgat
     3241 ttccggctgt gccctggtac gtaaggattc tgcgcctcat caacagctca aaccggaaca
     3301 aatcaaactg gccgatgata ttcatcttgc cagctccggc tggccgcagg cgcagtggtg
     3361 gaaacaactc aatgacccgc agttggatgc cctgatccaa cggacgctaa gtggttcaca
     3421 caccctcgcc gaagcgaaac tgcgggaaga aaaagcgcaa tcgcaggccg atttgttaga
     3481 tgccggttca cagttgcagg tggcagcgtt agggatgctt aaccgccaac gtgtctcggc
     3541 gaacggcttt ttaagccctt atgcgatgga tgcgcccgca ctggggatgg acgggccgta
     3601 ctatacggaa gccacagtag gtttgtttgc cggactggat cttgatttgt ggggtgtgca
     3661 tcgctcagcg gttgccgccg ccattggcgc gcataatgcc gcgctggcag aaaccgcagc
     3721 agtagagcta tcgctgacca cgggcgtagc gcagctttat tacagtatgc aggccagcta
     3781 tcagatgctc gatctgttag aacaaactcg cgatgtgatt gattacgcgg tgaaagcgca
     3841 ccaaagtaaa gtggcgcacg gtctggaagc acaagtgcct ttccacggcg cgcgggcaca
     3901 gattctggcg gtcgataaac aaattgctgc cgtcaaaggg caaatcaccg aaacgagaga
     3961 atctctgcgc gcattgattg gcgcgggagc cagcgatatg ccggagatca aacctgtggc
     4021 attaccacga gtccagaccg gcattccggc gacactctct tatgagttgc tcgccagacg
     4081 cccggatctg caagccatgc gctggtatgt tcaggcgtca ttagatcagg tggattccgc
     4141 gcgggcgttg ttctatccga gctttgatat caaagcgttt ttcggccttg atgccatcca
     4201 cctggatacc ttattcaaaa aaaccagtcg ccagttcaac ttcatcccgg gtctgaaatt
     4261 gccgctgttt gacggtggac ggttgaatgc caatctcgaa ggcacgcgcg ccgccagcaa
     4321 catgatgatt gaacgttaca accagtcagt actgaacgcg gtgcgtgacg ttgccgtcaa
     4381 cggcacgcgt ctgcaaacgc tcaacgacga gcgagaaatg caggctgaac acgtggaagc
     4441 aacgcgcttc acccagcgcg ctgccgaggc cgcctatcag cgcggcttaa ccagccgctt
     4501 acaggccact gaagcccggt tgccagtact tgccgaagag atgtcattac tgatgctgga
     4561 cagccgccgg gtgatccaaa gcattcagtt gatgaaatcg ctgggcggcg ggtatcaggc
     4621 ggctcccgtc gtcgagaaaa aataaaatgt ctgccgcgtg atggctgtca cgcggtattt
     4681 cgtttcgtca cgtcaaaact gacgacagcc tgtttttcgt cagagttttg aataaatagt
     4741 gcccgtaata tcagggaatg accccaaata aaatgtggca taaaagatgc atactgtagt
     4801 cgagagcgcg tatgcgtgat ttgattaact ggagcgagac cgatgaaaaa agtcgtcacg
     4861 gtttgcccct attgcgcatc aggttgcaaa atcaacctgg tcgtcgataa cggcaaaatc
     4921 gtccgggcgg aggcagcgca ggggaaaacc aaccagggta ccctgtgtct gaagggttat
     4981 tatggctggg atttcattaa cgatacccag atcctgaccc cgcgcctgaa aacccccatg
     5041 atccgtcgcc agcgtggcgg caaactcgaa cctgtttcct gagatgaggc actgaattac
     5101 gttgccgagc gcctgagcgc catcaaagag aagtacggtc cggatgccat ccagacgacc
     5161 ggctcctcgc gtggtacggg taacgaaacc aactatgtaa tgcaaaaatt tgcgcgcgcc
     5221 gttattggta ccaataacgt tgactgctgc gctcgtgtct gacacggccc atcggttgca
     5281 ggtctgcacc aatcggtcgg taatggcgca atgagcaatg ctattaacga aattgataat
     5341 accgatttag tgttcgtttt cgggtacaac ccggcggatt cccacccaat cgtggcgaat
     5401 cacgtaatta acgctaaacg taacggggcg aaaattatcg tctgcgatcc gcgcaaaatt
     5461 gaaaccgcgc gcattgctga catgcacatt gcactgaaaa acggctcgaa catcgcgctg
     5521 ttgaatgcga tgggccatgt cattattgaa gaaaatctgt acgacaaagc gttcgtcgct
     5581 tcccgtacag aaggctttga agagtatcgt aaaatcgttg aaggctacac gccggagtcg
     5641 gttgaagata tcaccggtgt cagcgccagt gagattcgtc aggcggcacg gatgtatgcc
     5701 caggcgaaaa gcgccgccat cctgtggggc atgggtgtaa cccagttcta ccagggcgtg
     5761 gaaaccgtgc gttctctgac cagcctcgcg atgctgacca gtaacctcgg taagccgcat
     5821 gcgggtgtaa acccggttcg tggtcagaac aacgtgcagg gtgcctgcga tatgggcgcg
     5881 ctgccggata cgtatccggg ataccagtac gtgaaagatc cggctaaccg cgaaaaattc
     5941 gccaaagcct ggggcgtgga aagcctgcct gctcataccg gctatcgcat cagcgagctg
     6001 ccgcaccgcg cagcgcatgg cgaagtgcgt gccgcgtaca ttatgggcga agatccgctg
     6061 caaactgatg cggagctgtc ggcagtacgt aaagcctttg aagatctgga actggtcatc
     6121 gttcaggaca tctttataac caaaaccgcg tcggcggcgg atgtcatttt gccgtcaacg
     6181 tcgtggggcg agcatgaagg cgtgtttact gcggctgacc gtggcttcca gcgtttcttc
     6241 aaagcagttg aaccgaaatg ggatctgaaa acggactggc aaatcatcag tgaaatcgcc
     6301 acccgtatgg gttatccgat gcactacaac aacacccagg agatctggga tgagttgcgt
     6361 catctgtgcc cggatttcta cggtgcgact tacgagaaaa tgggcgaact gggcttcatt
     6421 cagtggcctt gccgcgatac ttcagatgcc gatcagggga cttcttatct gtttaaagag
     6481 aagtttgata ccccgaacgg tctggcgcag ttcttcacct gcgactgggt agcgccaatc
     6541 gacaaactca ccgacgagta cccgatggta ctgtcaacgg tgcgtgaagt tggtcactac
     6601 tcttgccgtt cgatgaccgg taactgtgcg gcactggcgg cgctggctga tgaacctggc
     6661 tacgcacaaa tcaataccga agacgccaaa cgtctgggta ttgaagatga ggcattggtt
     6721 tgagtgcact cgcgtaaagg caaaattatc acccgtgcgc aggtcagcga tcgtccgaac
     6781 aaaggggcga tttacatgac ctaccagtgg tggattggtg cctgtaacga gctggttacc
     6841 gaaagcttaa gcccgattac gaaaacgccg gaatacaaat actgcgccgt gcgcgtcgag
     6901 ccgatctccg atcagcgcgc cgccgagcag tacgtgattg acgagtacaa caagttgaaa
     6961 actcgcctgc gcgaagcggc actggcgtaa tacagtcctt tctacagcct cctttcggag
     7021 gctgtttttt tatccattcg aactctttat actggttact ccctacccaa tcgtattatc
     7081 aaaatgaaaa aaattatcgc attgatgttg tttttgacat tctttgccca cgcgaacgac
     7141 tccgagcctg gcagccagta tttaaaggca gcagaggccg gggaccgacg tgcacaatat
     7201 tttcttgccg acagctggtt tagctccggc gatttgagca aagccgaata ttgggcacag
     7261 aaagccgccg acagcggtga tgctgatgcc tgcgcgctgt tggcgcagat caaaatcacc
     7321 aatccggtca gtctggacta tccacaagca aaagttcttg cagagaaagc ggcgcaagcg
     7381 ggcagtaaag aaggtgaagt aacgctggcg catattctgg taaatactca agcgggtaaa
     7441 ccggattatc caaaggcgat ttcgctgtta gaaaacgcct cggaagatct ggagaatgac
     7501 tccgccgtcg atgcccaaat gctgcttggt ttgatttacg ccaacggcgt gggcattaag
     7561 gccgacgatg acaaggcaac ctggtatttc aaacgcagct ctgcaatttc ccgaaccggt
     7621 tattccgagt actgggcggg aatgatgttc ttaaacggtg aggaaggttt tatcgagaag
     7681 aacaaacaaa aggcgctgca ctggttgaac ctgagctgta tggaagggtt tgataccggg
     7741 tgtgaagagt ttgaaaaatt aaccaacggt taaggtaggc ctgataagac gcggcaagcg
     7801 tcgcatcagg catccacaca ttgccggggg atatccccgg caatcttcaa ttattgatcc
     7861 gcagttttat caaatttgcc cagcacttca cgctcataag ccagcgcctt tttgcggtca
     7921 aatttgtgtt cccacttggc aatcaccagc accgccagcg cattacccac cacgttcagc
     7981 gcagtacgcg ccatgtcgag gatacggtca acaccagcaa taaacgccag accttccagc
     8041 ggaataccta cgctacccag cgttgccagc aacaccacaa acgacacgcc agccacgcca
     8101 gcaatccctt tcgaggtcac catcagcgtc agcaccagaa tgatttcctg ccagatggac
     8161 aggtcaatgc catacagctg agcgatgaaa atagcggcaa tactttgata cagcgtcgaa
     8221 ccatcaaggt taaaagagta accggtcggc accacgaagc tggtgatcga cgccggtgct
     8281 ccgtaggctt ccatcttctc aataattcgc ggcagtacgc tttcagagct ggcagtggag
     8341 tacgccagaa tcagctcatc tttcagaata cgaatcagga tccagacgct taacccgcac
     8401 agacgtgcca caattcccag cactaccagc gcgaagaaca gaatggcgaa atgcaccagc
     8461 agcaccagtt tcgccagtgg ccacagagac gagaaaccaa agttagccac cgtcaccgca
     8521 atcagcgcaa acacacccac cggtgcataa cgcatcacca tgtgagtcac tttaaacatg
     8581 gtttcagaga tggagcggaa cacggtcacc agcggttcac gatgcgtcgc gggcagggaa
     8641 gaaagcccca gaccaaacag caccgagaaa aagatgatcg gcagcatttc gcctttcgcc
     8701 atcgacgcca caatgttcgt cggcaccagc gacaaaatcg tgcccataat gccgtgggaa
     8761 ctgctttgta ccgcttccgt agtgctctga tatttcgaga tatcgacggt cgccagctgc
     8821 gacatatcca ccccggcacc gggctggaag acgttcgcca gagtgatccc caaaatgatg
     8881 gcgacggtgg tgatcacctc gaagtaaata atggttttcg cgccaatacg cccgagctgt
     8941 ttggcatcac caacacccgc gatacccacc accagcgtgg agatcacaat cggcacaaca
     9001 atcattttga tcagatggat gaagatatca cccgccggag agagcaaatt gacgaccagc
     9061 cagtcgcggc tgtcgctatg gtagtgcagg tagcttccca ggagaatgcc cagcaccata
     9121 gcaaacagaa tctgccaggc caggctgaat tttatatttt tcatcagaat aacttcctca
     9181 atggagcgcc gttccccgtt cagaaacgcg agaacgtgac atactgccag ggcatgaatt
     9241 gtgttgcgtt ggtttgtaga tttttttaac gcttatgatc agtatcattt gcgcggtatg
     9301 ttgcgcaacc ctttaacaac agagcttttc gctgcattaa tgcataataa cgcgagttta
     9361 tggcaggatt aatgaataac catttgttat ggaaagattt ttatttgttt aatctgcaat
     9421 aatactgatt ggtgattatt tttggcatat gttcattcga ttatttttta tgcagctcaa
     9481 tgacataatc ttcatcacca gatgattaat ccgatctccg ctgcaacagt tttgccatat
     9541 taatcgactc gaccagctgt gtccggttga tccggggtga gttgagatcc atcacttttt
     9601 gccatagttc gatggcttgc gcgtaattcg cctgcataaa cgcatcggaa gccagcagca
     9661 tcagagcggt gatttcgtta gagtccagcg cgagggcttt gtcgatcatt gcgcgagtct
     9721 gggcggtcat atgctggctg gcctggtaat aaagcaccgt cgccagcgcc gcatacagtt
     9781 cagcgttctc accacgtagt tgcaacgcct gacgatacgc cagcagcgaa ttgctgtaat
     9841 cgttttgcca cagatagtac tcgcccagta acgcccattg ttcgctgttt tgtggattag
     9901 cgcggatttt atcctgcaat gcctgaagct gcgcttccgg ggtttgctgg ctggcaaact
     9961 gatgtagcgg atcgcgctga cgctgatact ccgcacgtac cgcctgccat tttgggctta
    10021 acagataact gccgacacag aggaatacca tcaatatcgt gagtgttgtc agcattttca
    10081 ccggtatttg aggctgtttc atcgcttagc cctcactcgc cagaggatca gtgccatcag
    10141 caataacaac accactggca gcgcccacaa caccagcgtc tgacccgtta acggtgggtt
    10201 atagcgaaca aaatctccgt agcgttcggt catccagcca atgatttcga cttcgttctt
    10261 cccctccgac accatgctat aaacctgatg gcgcatactg accgccaccg gcgcattgga
    10321 ttcgagtaag ttttgattct ggcactgcgg acaacgtaac tggctggcaa tatttaacgc
    10381 ctgttgctgt tgttgcggat tggcgaattg ccaggtgtct acaacctgag cgcgcgcaaa
    10441 acaggtaaat aacagcagta gcgtgagaag ccctttattc atcgcgcttc ttcccccgcc
    10501 atccgcttaa caatgcgccc gcaatcatca acaatcctcc cccccagatc cagcgcacac
    10561 cgctttgtac atacaaacga aaagcgtaac gatccgctct ggttttttca cccatgaccg
    10621 cataccagtc atggatgccg ttccagcgaa ttgacggttc catcatttgc tgacggcgtg
    10681 cttcataaaa acgccgctcc ggcgttaatt caccaatgcg ttgctgatgg tcaaacagcg
    10741 ccactatcgc tttttcgctg gtgtaattgc ctctggcttg cagatcgagt cgctcaaaac
    10801 ggaaggtgta tcctgccagc gtcacctgct gacccggctg taaattcagg ctgatctcct
    10861 gacgactgac actggaaacc acgatccccg cggcaaataa cagcacgcca gcatgagcta
    10921 ccagcgccgg aagctgcacg cgtttgccag agacaaacgt cgccagcaca atcaccacca
    10981 gcatcaacag accaaacggt aacgtcgcgc ggttaaaata cggcgcgcca acggagaggc
    11041 gtccccagcc cagcaggccg taaatcatcg gataaagcgt tcccaccagc acgatcagta
    11101 gcactgcgca aaacagcaac agcgtagcga ggattaacat ttcccgcgat aaccccgaaa
    11161 aacgcaccgc cgggccaccg tcccgcgctc gccagccata cagagccaaa gacgcaaggc
    11221 taatcagtgc aaacaggctg aacaacggca cggcgcggac gttatccagt gcgaacgcat
    11281 gaaccgaaac cagaatgcca gaacggacaa ttaaggtgcc cagcagcgac agcatcagag
    11341 taactatcgc cagcaacagc gaccagtggc ggaaaatccc ccgctggcgt gtcagggaca
    11401 aactgtgcag cagcgcagtg gcagaaagcc agggtaataa agaggcgttt tccaccggat
    11461 cccagaacca ccagccgccc cagccgagtt cgcagtaggc ccaccaggaa ccgaggatga
    11521 tccccgccgt taatgcactc cagccaggta acgcccagcg ccagcaaatt cgggcgctgg
    11581 ccgcatcaaa ctcgccgcgc agtagactcg ccagcgccac gctcgccgct accatcaaac
    11641 cgccatagcc aaggtaaagc agcggtggat gaaagataag accgggatgt tgcagcatcg
    11701 gattgagatc gcggccttcg attgctggtg gaaatatccg cacgaaggga tcggaccaca
    11761 gcaccacaaa cagtagcagt gcggcgagca tgagagataa aacggctagc gtcagcggaa
    11821 atagcggatc gctttgctgc cgataatgcc aggcaaacag cgcgctccag gcggaaagca
    11881 gcagcaccca aagcagcagc gaaccttcat gaccgcccca caccgccgcc agtttgagtt
    11941 cccacgacag caggctgtag ctatgttggg cgacataaat gaccgagaaa tcgctgatga
    12001 gaaaacaata cgtcagtacg ccaaaggcga gcagcaggag cgcgaactgc gccagaatgc
    12061 cgatgcaagt gagtcgcatc atggcaggcc agcgcaaccg cactcccgca aaggccgtca
    12121 acggggtcaa cacgttgacc ccgagactta acaacaacgc cagaaaaccg acttcaggaa
    12181 ggaatacgtc cagaaaccac ctcttttctg gcttacgcca ccgtcaactg tccggcgtag
    12241 agaatgaaaa agcgcagcat caacacgccg accagactgg ccccacacgc cgccagcacg
    12301 gcaggaatgc cggaactgcg attgacccac ggtttgagca acattggcac aatcagcccc
    12361 agcccggcga caccaagcca gaaccaccac gtccagaaac cgccacctaa tgccgccacc
    12421 agcgcacgaa ctttaccgtc atcgcccagc gccagaccga caaaaaacgc caccagcagg
    12481 aagatttcac cccataccac ggggatttcc atccggtgaa caaagtgcgc ttccgtggaa
    12541 tgcgggttac tgcgttggcg tatcgccatg gcgatcagcg ccaccgccgc accggacgag
    12601 atgccggaga agaggagcag caccggcagg ctcgggttat tgaggaacgg atacgatttc
    12661 agcgccgaca gcagaaagcc ggtataagcc cccagcagca ccgccaacac cagcatcagc
    12721 gtttccagtc cgcgatgaac gggcgtcagc aggctcagaa ccttttgcac gatccccagc
    12781 ttcggcaacc agcgttgttg cagggcaagc aaatcatgtt caaagatttt cgccagccac
    12841 agcaccagca ccaccatgta gagctgaaac agcatcaccc ccatcgacat caccgaggta
    12901 aaactgtagt ggaacatcag cttccagaag gtccacggtc ttgtcaggtg gaagacgagg
    12961 atcaacagac cgaggatcac cgcgcccggc ccgacaatca gcgtggtgcg cagcaacgta
    13021 ctgtctgcac cgcccgcctg cgggtagaag cgacgtaaca gcacggccag cgtcaccaga
    13081 ccggcagaaa taccaatcaa aaacaggtag atggcaatcg gccagtccca caccagcgat
    13141 tcaaaatgaa atgcggaagt ctgcgtcatt ggctcacctc cccgtattta aacggtacgc
    13201 ggtacagctt cggtttggtt cccagcgcca gcttgtagcg gtaagtaggc ttctggcgca
    13261 gcagttgcga aatctcactg ttaggatcgt ccagattgcc aaacgtcagc gccttcgtcg
    13321 ggcaagcttc aacgcacgcg ggaagcttac cggcctgcaa attggtctta cgacagaaat
    13381 cgcatttgtc cgccgtcttc gtgaccggat ggataaagcg cacgcggtac ggacaggcgg
    13441 cgatgcagta ctgacaaccg acgcagagat caggattaac atcgacaatc ccgctagcgg
    13501 catcacgaaa agacgcaccg gtcgggcaga cgtcaacgca cggcgcatga tcgcagtgct
    13561 ggcaagactt acggaagaag cgatatttca catcaggaaa ttcgccctgc ggctcgctac
    13621 gaataatcgt caggcgcgag acgccttccg gcaccttgtt cacttcccga caagcatcca
    13681 tacaggcggt acagccgatg cataacgact cgtcatgcac catgccgtaa cgcaccccat
    13741 tgatattcaa cgttttcgcc accacgcgcc ctgcggtgcc gctgacggct gccagcacgc
    13801 cgacgccggt gagaaactgg cgacgagacc aggtcatggc tgctccttaa gcaacggaac
    13861 agacgccggg ttaaagttcg gattggtgcg ctgatcgctg tggcaatcga cgcaaatctt
    13921 aatccgtcct ttgtcactta acgtctgcat cgtatcttgc tgtggatgca gggaatggca
    13981 gctggcgcac gccactttgg ttacgtggac atcgtgcggc cagaacgctt tttgcaactg
    14041 ttccggcaga tgacaggaca tacagacgct gttctgctct ccgaccttat acatcggctc
    14101 gttaaagcgc atcacatctt tcaccccttc gcggtgttgc ggtgatgctt ggccgtgaca
    14161 gttggtgcag gtgaccggca atttgttatt cgggttgata acggaagcat gtttgccatg
    14221 cataccttcg gtatctggtt tatgacagtc cagacaggcg gcatccgggt tacgctgctg
    14281 ggtaacttcg tagcgatcac cagatgcctg caccgcaggg gtagcggtaa ttccgttcag
    14341 gctccacaac aggcccgacg ccagcacccc agcagttaat aacgaacgta atacgctcat
    14401 attcactcca ttgtgttttc cgccccttgc gaggcggaac ggggttattg gcttaacaga
    14461 ccgtttttac gtgcctgctc ttcccactgc gggatcaccg ttttgatgaa gtcctgcttc
    14521 tcggccttga tttgttccat gttcaggcca atggcctgct gggctttctc tttggtcgag
    14581 atatccggga tctggatttc atgggtgata cctttggtcg ccagcaggcg cgccagtttg
    14641 gtgcgcgcat ccgccgcttt atccatcgcc gtaccgagca tccgtagacc ttcttccggt
    14701 gcgtgcatat gaataccgtg ggaagcaatc gccagatccc aacgccactg ggcatgacgg
    14761 atatcgtcct gaatcggctt catttcggct tccgtcgcgc ctgcatccag cgccgctttc
    14821 gcttcgaagt gagcgtgaac cagttgatct tcaaccttga ttttcaggtc gttaatcgat
    14881 tgcttacgtt ccgcgaccac tttttgcaag gcagctttat cctgggtatg gcagttcgca
    14941 caagtctggg cgaagttatc aaacggatta ccaattttat ggtcggtgta gagtttgcct
    15001 tcggcgttct gcacttttgg catatggcag tcgatacagg tcacgttgtt tttaccgtga
    15061 atgcccgctg tccaggtttc atattccggg tgctgcgctt tcagcattgg cgttttcgac
    15121 agggagttag tccagtcaga gaaggcaatt ttgtcgtaat actgctccat attttcgact
    15181 ttcatgccgt catcccacgg gaatttaacc gctttgtttt tgccgtcgaa gtaatactcc
    15241 acatggcact gaccgcaaac catcgattgc tggtcgaaac gtccggcttt ctcaaaaggt
    15301 ttaccaatgg cttccatcgc gcgagccgca tacggacggg aaagggttaa ctccggtttg
    15361 cctttggcga actctggtga ggcggtgtta tggcagtcgg cacaacctaa gttgttgacg
    15421 atttccgggc cgccgcgcgc ccatttaccg tggaagtagc catcttcgcc gtctttctgg
    15481 atcagacgcg ccacatccgg gcttttacaa ctccagcacg ccatcggtag cggaccatct
    15541 tcagcgtttt tcggcgcacc ggtacgcagg gtttcacgca catcggtcac agcaaaggca
    15601 tgtccacgag gcttgttgta atcgcgcgag aagggatacc ccgcccacag gatcaccagc
    15661 cgtggatctt ccgccagggc gtcaacacgc tctgactgtt ccgaggtggc tttccaggag
    15721 agatattgat cgtgatgctg cggggcaaag gtttcattct tcgcttccac agttacaggt
    15781 tttgcgggag ctgccgtttg ttcagcgtga acagaagtga aaaagaaaaa aggaatcaat
    15841 aagctgaaga tacggcgtgc gtttattttt atccttgtca taggggcttc atccgaattg
    15901 cgccattgtt gcaatggcgg tttttattgt ttttcacgac agtaaccgca cctacactgt
    15961 catgacattg ctcaccccta tgtgtaacaa ataaccacac tgtgaatgtt gtctttaatc
    16021 aattgtaagt gcatgtgaaa taccacttta gagttagtca gtatcttcct ctttttcaac
    16081 agcatgcata actgcatgtt cctcaaagaa ttaatcaact tttgttggtg accttcaaaa
    16141 attaccctgc cgtttatttg caccattcta cttttgcgtg atctgtcgcc caaatactaa
    16201 acaaaactgc caatacccct acatttaacg cttatgccac atattattaa catcctacaa
    16261 ggagaacaaa agcatgagcc aaattcacaa acacaccatt cctgccaaca tcgcagaccg
    16321 ttgcctgata aaccctcagc agtacgaggc gatgtatcaa caatctatta acgtacctga
    16381 taccttctgg ggcgaacagg gaaaaattct cgactggatc aaaccgtacc agaaggtgaa
    16441 aaatacctcc tttgcccccg gtaatgtgtc cattaaatgg tacgaggacg gcacgctgaa
    16501 tctggcggca aactgccttg accgccatct gcaagaaaac ggcgatcgca ccgccatcat
    16561 ctgggaaggc gacgacgcca gccagagcaa acatatcagc tataaagagc tgcaccgcga
    16621 cgtctgccgc ttcgccaata ccctgctcga gctgggcatt aaaaaaggtg atgtggtggc
    16681 gatttatatg ccgatggtgc cggaagccgc ggttgcgatg ctggcctgcg cccgcattgg
    16741 cgcgatgcat tcggtgattt tcggcggctt ctcgccggaa gccgttgccg ggcgcattat
    16801 tgattccaac tcacgactgg tgatcacttc cgacgaaggc gtgcgcgccg ggcgtagtat
    16861 tccgctgaag aaaaacgttg atgacgcact aaaaaacccg aacgtcacca gcgtagagca
    16921 tgtggtggta ctgaagcgta ctggcggaaa catcgactgg caggaagggc gcgacctgtg
    16981 gtggcacgac ctggttgagc aagccagcga tcagcactag gcggaagaga tgaacgccga
    17041 agatccgctg tttattctct atacctccgg ttctaccgga aaaccaaaag gcgtactgca
    17101 cactaccggc ggttatctgg tgtacgcggc gctgaccttt aaatatgtct ttgattatca
    17161 tccgggtgat atctactggt gcaccgccga tgtgggctgg gttaccggac acagttattt
    17221 gctgtacggc ccgctggcct gcggcgcaac cacgctgatg tttgaaggcg taccgaactg
    17281 gccgacgcct gcccgtatgg cgcaggtggt ggacaagcat caggtcaata ttctctatac
    17341 cgcacccacg gcgatccgcg cgctgatggc ggaaggcgat aaagcgattg aaggcaccga
    17401 ccgttcgtcg ctacgcattc tcggttccgt gggcgagcca attaacccgg aagcgtggga
    17461 gtggtactgg aaaaaaatcg gcaacgagaa atgtccggtg gtcgatacct ggtggcagac
    17521 cgaaaccggc ggtttcatga tcaccccgct gcctggcgct accgagctga aagccggttc
    17581 ggcaacacgt ccgttcttcg gcgtgcaacc ggcgctggtc gataacgaag gtaacccgct
    17641 ggaaggggct accgaaggta gcctggtgat caccgactcc tggcctggtc aggcgcgtac
    17701 gctgtttggc gatcacgaac gttttgagca gacctatttt tccaccttca aaaatatgta
    17761 tttcagcggc gacggcgcgc gtcgcgatga agatggctat tactggatca ccgggcgtgt
    17821 ggacgacgtg ctgaacgtct ccggtcaccg tctggggacg gcagagatag agtcggcgct
    17881 ggtggcgcat ccgaagattg ccgaagccgc cgtagtaggt attccgcaca atattaaagg
    17941 tcaggcgatc tacgcctatg tcacgcttaa tcacggggag gaaccgtcac cagaactgta
    18001 cgcagaagtc cgcaactggg tgcgtaaaga gattggcccg ctggcgacgc cagacgtgct
    18061 gcactggacc gactccctgc ctaaaacccg ctccggcaaa attatgcgcc gtattctgcg
    18121 caaaattgcg gcgggcgata ccagcaacct gggcgatacc tcgacgcttg ccgatcctgg
    18181 cgtggtcgag aagctgcttg aagagaagca ggctatcgcg atgccatcgt aacccacaat
    18241 tgccggatgc gaatcggtaa cggtttgtag gcctgataag acgcgacagc gtcgcatcag
    18301 gcattgattg ccggatgcgg cgtaaacgcc ttatccggcc tacattcggc aagggttacc
    18361 cgagcgttaa ccttctccca taagggagcg ggaattaaaa caatccctac attacctctg
    18421 gagaatctgt gatgaatggt acgatttatc agcggataga agacaatgcg catttcaggg
    18481 agttagtcga aaaacggcaa cggtttgcca ccatcctgtc gattattatg ctggcagttt
    18541 atatcggctt tattttactg atcgccttcg cgcccggctg gctgggcacg ccgctgaatc
    18601 cgaacaccag cgtcacacgc ggtattccaa ttggtgttgg agtgattgtg atctcctttg
    18661 ttctcaccgg tatctacatc tggcgggcga acggcgaatt cgaccgtctt aataatgaag
    18721 tcctgcatga ggtacaagca tcatgaaaag agttctgacg gcgcttgccg ccacactccc
    18781 tttcgcagct aacgccgcgg atgctattag cggggccgta gagcgccagc caacgaactg
    18841 gcaggcgatt attatgttcc tgattttcgt cgtgtttacg ctcggcatta cctactgggc
    18901 atcaaaacgc gtacgttctc gtagcgacta ctacaccgca ggcggcaata tcactggctt
    18961 ccagaacggg ctggcgattg ccggggacta tatgtccgcc gcctcattct tggggatctc
    19021 cgcgctggtg tttacctccg gctatgacgg gctgatctac tcgttgggct tcctggtggg
    19081 ctggccaatc attctgtttc tgattgccga acgtctgcgt aacctgggac gctacacctc
    19141 tgccgatgtg gcctcttatc gtctgaaaca agggccgatt cgtattcttt cggcctgtgg
    19201 ttctctggta gtggtggcgc tttaccttat cgctcagatg gtaggcgcag gtaaactgat
    19261 cgagctgctg tttggcctta actatcacat tgcggtggtg ctggtcggcg tgctgatgat
    19321 gatgtacgtc ctgttcggcg gcatgctggc gaccacctgg gtacaaatta tcaaagctgt
    19381 gctgttgctg ttcggtgcca gctttatggc ctttatggtg atgaaacacg tcggctttag
    19441 cttcaacaat ctgttcagcg aagcgatggc ggtacacccg aaaggtgtcg acatcatgaa
    19501 accgggcgga ctggtgaaag atccgatctc cgcgctctct ctggggctgg gactgatgtt
    19561 tggtacggcg ggcttgccgc acattctgat gcgcttcttt acagtcagcg atgcccgcga
    19621 agcacgtaag agcgtgttct acgccaccgg atttatgggc tacttctata ttctgacctt
    19681 tattatcggc ttcggcgcga tcatgcttgt tggtgcgaat ccggaatata aagacgcggc
    19741 gggccatctg attggtggta acaacatggc ggccgttcac ctggcgaatg cagtgggcgg
    19801 caacctattc ctcggtttta tttcagcggt tgctttcgcc actatcctcg cggtggttgc
    19861 ggatctgacg ctggcgggcg catcggcggt ttcgcatgac ttgtacgcta acgtcttcaa
    19921 aaaaggcgcg accgaacgtg aagagctgcg ggtatcaaaa atcaccgtac tgatcctcgg
    19981 cgtgattgcg attatcctcg gcgtgctgtt tgagaatcag aacatcgcct ttatggtagg
    20041 ccttgcgttt gccatcgcgg cgagctgtaa cttcccgatc attctgcttt ccatgtactg
    20101 gtcgaaactg actacccgtg gcgcgatgct gggcggctgg ctggggctga ttaccgcagt
    20161 ggtgctgatg atcctcggcc cgactatttg ggtacagatc ctcggtcacg aaaaagccat
    20221 cttcccgtat gaatacccgg cgctgttctc tatcagcgtg gcattcctcg gcatctggtt
    20281 cttctcggca accgataact cagcggaagg ggcgcgcgag cgtgaactgt tccgcgcgca
    20341 gtttatccgc tcccagaccg gctttggcgt tgagcaaggc cgcgcacatt aatctctccc
    20401 cttccccggt cgcctgaccg gggaatacgc ttcctctcca acatgcatca ccttttccca
    20461 taatattaaa caaataaact cattaaaaaa tgagcgattt ttgacagtcg tagaaaatga
    20521 taatgcagag aatatgcctt ttctttcttg ttaattataa ggatatttta tgcgctataa
    20581 tggtttaaat aatatgtttt tccctctttg ccagattaac gataaccact ctatcacaag
    20641 ttcatcacat acaaagaaaa caaaatcaga taattacagc aaacatcata aaaacacgtt
    20701 aattgacaat aaagccctct ctcttttcaa aatagatgat catgaaaaag tgataggctt
    20761 gattcagaaa atgaaaagaa tttatgatag tttaccatca ggaaaaatca cgaaagaaac
    20821 ggacaggaaa atacataaat attttataga tatagcgtta tatgcaaata ataaatgtga
    20881 cgatagaatt acgagaagag tttaccttag taaagaaaag gatgtatcca ttaaggtggt
    20941 atattttata aataatgtcg ccgtccataa taatactatc gaaattccac agatagtaaa
    21001 tggtggttac gatttttcac accttagcct gaagggtatc gtgattaaag atgaagattt
    21061 atccaattcg aattttgcag gttgcagact acaaaacgct attttccagg actgtaatat
    21121 gtataaaacg aatttttatt acgccataat ggaaaaaata ctttttgata attgtattct
    21181 cgatgactca aatttcgctc agataaaaat ggccgacgga actctaaatg catgctccgc
    21241 tatgcatgtt caattctaca atgcagcaat gaatagagcc aatattaaaa ataccttcct
    21301 tgactattca aatttttata tggcgtacat ggctgaggta aatctttata aagtaatagc
    21361 gccatatgtt aatttattta aagccgacct tagtttctct aaactcgatt taattaactt
    21421 tgaacatgct gatctgtctc gcgtcaatct gaacaaagca atcctccaga atataaactt
    21481 aattgatagc aaactctttt gtacgtggct gacaaataca ttcctcgaaa tggttatatg
    21541 taccggctct aatatggcta atgttaattt taataatgcc aatttaagca actgccattt
    21601 caactgttct attttaacaa aagcctgtat gtttaatacc cgtctctatc gggttaattt
    21661 cgatgaggct agcgtccagg gaatgggcat ttccattctc cgtggggagg aaaatatccc
    21721 cattgatagt gataccctgg taacactaca gaaattcttt gaagaagatt gtacctctca
    21781 tactggcatg tcacaaactg aggataatat taatgcagtc gctatgaaga ttactgcaga
    21841 tattatgcaa cacgcagatt gaggttaagt cgaagaacag ccaatgaatt gactttacaa
    21901 gagagataac ttatttgttt tagaatcatg aatgttcgcc cgagctaacc tgttaaaggt
    21961 gcaaaagatt gcaccttttt cagattacct cgggcctggt tacgtcgctt tcggcttact
    22021 gattttcctc aattagcaac gcttcaagca aatcgagatc gtgcagtaat ttttgcagcg
    22081 tttcgttgct gatctcccgc gtggcgcgca ggtggtacag ttcagcacgt tcagaacgca
    22141 atgccgccag acggaagcga cgctcaaggt tctcttcctg cacggaactt tcaacgtcgt
    22201 tacgtccatc ggcgcgacga cgcaggttac caatgacgcg agaactgacc tccgtaagca
    22261 actggttatc gatattctct tcggtatccg ccgccagacg ctcctccatt ttctggatcg
    22321 ccacaatcgc cacttctgcc gttgccgctc gcgcaatacg ttcttctttc aattgttgcg
    22381 aatggtccgc gacttcaatg tgttgtagca gaataggcaa catcaccacg ccgacaaaca
    22441 gcgagaagag aatgacgcca gccgccagga acaccagctc atagcgcgcc gggaagacgt
    22501 taccatccgg caagagcagc gggattgaga gcacaccggc cagagtgatc gccccacgca
    22561 ccccagcgaa agacgcgatc aggatttctc gtgtggtcca cgaaccaaac tccatcggct
    22621 tcttcttcag gaagcggttg ctgaactttt tcatcgtcca cagccagccg aaacggacca
    22681 gcatcagcgc cgcatatatc agaataatat cggtaaacag catccagatt tcgacgttag
    22741 ggtcgatttc tgccgccatc agcgacgtct ccagaatacc cggcagttgc agacctaaca
    22801 gcaggaacac catgccgtta aagacaaatt ccagcatcgc ccaggtgctg tttgcgcgca
    22861 ggcgcattgc cagtggcgca cggcgcatca caccggagcg ggtgatggtc atcccggcgg
    22921 caaccgcagc gaggatgccc gaaacgccaa tatgttcggc aatcagataa gaagcgaatg
    22981 gcagcaagaa cagcagaacg atctgcgtcg cgggttcatc accgccccag cggctgagga
    23041 atcgcagcga gcgaccatac agccagctca ccacaaaacc ggcaagaata ccgccaatgg
    23101 ctactttcat aaattcgacc gtcgcaccgc cgacagtgaa gatcatcgtc cccatcgcca
    23161 ctgccacggc aaacttcaac gacaccaggc cggaagcgtc attcatcaac gcttcgccct
    23221 gcaaaatgcc catgattttt ttcgggatgc gcccttcgcc aacaatcccg gagagcgcca
    23281 cagcatcggt cggagaaagt accgccgcca gcgcaaaggc ggggatcagc ggaatgcccg
    23341 gcaccaccca gtaaataagg aagccaatgc cgaccacggt gaccaccacc agcgccagcg
    23401 cgaggccgaa aatctctcga ccatgttcga gaaattcacg ggtcggcgtt ttccagccat
    23461 cagcgaacag caacggcggg ataaacaaga ctaaaaagag ttcaggatca aactccacat
    23521 gcaaaccaaa cgtcggccac gccagtagcg caccgatggc gatttgcata agcggaagcg
    23581 ggatctgaaa gggcatgaca cgagtgacca ccccggacag cgagaccacg agggtcatta
    23641 tcagtatggt gaagaagatt tccatgcgtt ccctgtttgc gatgtttttt atgatctgag
    23701 aaagacgtta tgcctgaatt ctcatttttt cagaatatcg caaaagctca cacaatgtgc
    23761 ggagaaaata aaaacgggca gccattgcca cccgttttca gaatccgatg aaagattaga
    23821 tagcccagcc acccgcatag aaggtcacca gcgccacggc gataacgacc gtaccgatgt
    23881 tcaacttgcg ccattcaccg gaaaccagac gaccaatcac cagagtcgcg aagccgatca
    23941 tgatgcctgt tacgatgtta caggtcagca cgatgaatac cgccgtaacc agacccgcca
    24001 tcgcatcaac aaaatcagca aagtcgattt tcgccacgtt gctcagcatc agcaggccaa
    24061 cgtacatcag cgccggagcc gttgcatacc ccggaacgag gtaagaaagc ggagagagga
    24121 acagaatcag caggaacagc acgccaacgg tgatagccgt caacccggtt ttaccgcccg
    24181 ccgccgtacc cgccgcagac tcgatgtata ccgctgccgg agctgcaccc accaggccag
    24241 agaaaacgct gctcatggag tcagtggtca gtgctttccc gccgtcgatg atctgcccat
    24301 ctttatccag caggttcgcc tggccggcga cagcgcggat agttccggtg gcatcaaata
    24361 ctgccgtcat caccagcgcc agaacgcttg gcaggactac aggattcagc gcgcccataa
    24421 tgtccaggct gccaatcagt gaattgccgt tttcatcgct caatgaaggc atggcgaaaa
    24481 cgccggagaa atggacgtta ggatcgaaga tcaaaccgac aattgagata ccgataatgg
    24541 tcagcagaat gccaccaggg actttcagtt tttccaagcc gatgatcacc gccagacctg
    24601 ccagtgacat aatcaccggg aaggtcgcga aatcacccag cgcaacgggc agaccatcaa
    24661 gcgggttttt aatcaccaga ccgacaccat tagcggcaat gagcagcagg aacagaccga
    24721 taccgatccc cgtgccgtgc gccacaccgt gcggcaagtt gcgcaaaatc cagctacgga
    24781 tacccgtggc agaaattacc gtaaacagca cacccatcag gaacacggca cccagcgcga
    24841 caggtacgct aatatgttgc cccagcacca ggctgaatgc ggtaaacgcc gtcagggaga
    24901 tggcgcaacc aatcgccaac ggcagattag cccacagacc catcacgata gaaccgagtc
    24961 cggcaaccag acaggttgca acgaaaactg ccgcaggcgg gaagcctgct ttacccaaca
    25021 tacctggaac gacgatgacc gagtagacca tcgccagaaa cgttgttaac ccggcaacca
    25081 cttcctgacg gacagtgctt ccacgttgtg aaattttaaa ccaggcgtcg agtgaaccgc
    25141 cggtacgcgc tgatggcgta gacataagaa aacatcccct gagagtttaa ttttcgtcaa
    25201 gatgcgtggc ggacaatccc ctgccagcga aacgttatct tcctgctcca ggtttgcggc
    25261 aatttggtgc gtcgcaaaaa agcaaacgtt tagctccgcg catacaaaac gggtggtaaa
    25321 aaaaagcaaa cgattatccg gcgatcatag cggctttttc aaccgaagtt tgctgctttt
    25381 ttctctttta ttgtcttgac gatgaaaaac caggcaattg atgcgcaaac gttatcgtcg
    25441 atgtgcaaga ttgcagcgtt atcgagccgt ataccgttct ggattgcgat aaaaaatcgt
    25501 ctgagatgac aaaaatattg cggctaaaaa gtatactttt acactggcga agaaacgtcg
    25561 gtgacataca attaaagcat caacaccaac cggaacctcc accacgtgct cgaatgaggt
    25621 gtgttgacgt cgggggaaac cctcctgtgt tccagcggga tagagagaaa gacaaagacc
    25681 ggaaaacaaa ctaaagcgcc cttgtggcgc tttagttttg tgtttgcgga taattccaaa
    25741 agagctcgat cagtcccctc gccccttcgg ggagagggtt agggtgaggg gaacaggcag
    25801 caccagtgca aatatttacc ctcaccccgg ccccatagtt aacgatgagt cagcttgtcc
    25861 ttgtggcgct ttagttttgt tcatcttcca gcaagcgtgc gccggtacct tcttctccta
    25921 agcggtcgcc cgggttacgc aacgggcaat cactgcgcga aaggcagcca caaccaatac
    25981 atccgtccag ttcgtcacgc agcgccacta aggtatgaat gcgccgatcc aactcttctc
    26041 gccattggga cgaaagctgt ttccactctt tcgcacttaa cgtatgccct tctggcaaca
    26101 cgccaaacgc ttcaccaatg gtcgccagcg gaatgccaat acgctgagcg attttgataa
    26161 ttgcgacata tcgcaacaca tcacgtttat atcgccgctg attgccgctg ttacggatac
    26221 tagtaatcaa ccctttgctt tcatagaaat gcagcgccga taccgccaca ccgctgcgtt
    26281 tcgccacttc gccaggggtt agcagcgctt taatgcgggg taatttcttt tccataaatc
    26341 gctttacctc aagttaactt gaggaattat actccccaac agatgaatta acgaactgaa
    26401 cactgaaaag aggcagattt atgtcccatc agaaaattat tcaggatctt atcgcatgga
    26461 ttgacgtgca tattgaccag ccgcttaaca ttgatgtcgt cgcaaaaaaa tcaggctatt
    26521 caaagtggta cttgcaacgg atgttccgca cggtgacgca tcagacgctt ggcgattaca
    26581 ttcgccaacg ccgcctgtta ctggccgccg ttgagttgcg caccaccgag cgtccgattt
    26641 ttgatatcgc aatggacctg ggttatgtct cgcagcagac cttctcccgc gttttccgtc
    26701 ggcagtttga tcgcactccc agcgattatc gccaccgcct gtagttttat tgcccgcgcg
    26761 ttaactcccg cgcgggtaat tgctccatcc attgcataaa cacctgcggc ggcatcgcct
    26821 tcgcaaaaaa ccatccctgg caatagcgca cgccgcgttt gcgcagccag ttaacctgct
    26881 cctcagtttc gacgccttca gcgatcgttt ttaaccccag gctgtgcgcc agctcgatga
    26941 tgtgttccgc aatcaaatga ctggttttgt gggtggtcag cgtttcaaca aacgatttat
    27001 cgattttcaa aatatcgaca ttcaatgatt taaggttatg caagttagag tagccaatac
    27061 caaaatcatc aattgccact tcgtaaccag cctggcggaa agccagaatt aatcggcgtc
    27121 attttttcga catcaagaaa tgcatgctca gtcacttcaa atttaatttg ctgcggacgc
    27181 accgcgtatt gctctgtttt ctgattgatt cgcgctatca accgtgacgt atgaaaatcg
    27241 gaggccgaca ggttaataga aacatagcga tcggcatgtg ttgccaggta cgcgcccaga
    27301 tcgcggaaga cattatcaat aacataatca gttatctgtt ctatcatccc ctccttttct
    27361 gccagcggaa taaactctgc cggattcatt atttgcccct gctcaccagg ccaacgtaac
    27421 aacgcttcag cgccgataca tttttctgtt ttgatatcga ttattggctg gtaataaaga
    27481 caaagttgat gtttttcgag ggcgcgttgc aatttacgtt tgggagataa atagttttgt
    27541 cgaatacgta gccagagtaa tagcagaaca agactcccca aaataccggc gggcaacgca
    27601 aaaataagat gattataaaa atgggttata agacgttgat atgaagtcgc aacaatggct
    27661 gcaattgggc gttttgtcga ataaattgtc gcatataaat agccatttct ttgtacggtt
    27721 aaatccttca aatgaatcag cggagaaaac gttgctgccg aggcctcttt gcttaacgag
    27781 aaaaaggttt tcatcaccgt atcatacaca ccccattgca atgtcgggtc atcagacatc
    27841 acttcactcc agaagagagg gttgataacc gccacataat ttccccgctg catataggtc
    27901 attttatagc cagagaaaaa aggcgtatcg cggtaataat agatagaaac gttaggttca
    27961 cgcttataat cggccggtgc aatcgtatag ccgtttacag gcgctatcag cgatgagcat
    28021 aaaaaatggt tatcacgaat tcattaatat aaagatagcc acgaataata ttcaacattc
    28081 gcttttgatg ggctggagtg caaacttgcc cctgatagcg ttcagctgca tcgctcacta
    28141 aatctgcctg gcgaatgacc agctcggatt tatccagtgc cagttgagca aaggttcgga
    28201 gctgattatt cacttctgat tttgcccaaa ggaaggcaat ccatagcgaa agaatgatgg
    28261 gaaagagaac taaaaagata atgcccggca acgccagtaa ttggtgtcgt gcacgatgac
    28321 tcattgctat caatttcctg cctgtttata cccggccatt gcaaggtgaa accctggcta
    28381 ccgacgtctt tagataaatc ttacggtata aatagcactt ttgcctgatt tgaaattgat
    28441 acctttttaa tcgtcacctg gatgaaaata attcattgat attcaaaatt aattttctac
    28501 aggaatacag aggcggcggg aagatatgcc cttaatgcca ctgaaaaacc acataatttg
    28561 tacagcaata aaagtcacgg cctaatatgg ctaacaaaca atcaattgtg atatagttca
    28621 caaaattaat gaaacaaaca gagtgtttca tttttgtgtt ctgcaagccc cgcgatagcc
    28681 agagtgtacc acgcctcccg tgaacaacgc cgcgctgtcc agggcatcgg ctcttttcag
    28741 aggaattgtt tgatggctac cctcaccact ggcgtggttc ttcttcgctg gcaacttctt
    28801 agtgccgtaa tgatgtttct ggccagcaca ctcaacatcc gttttcgtcg gtctgattat
    28861 gtcgggcttg cggtgatcag cagcggtctg ggcgttgttt ccgcctgttg gttcgcaatg
    28921 gggttgcttg gcatcacaat ggcggacatc accgctatct ggcacaacat cgagtcggtg
    28981 atgatagaag agatgaatca gacaccgcca caatggccaa tgattttgac ctgatacgta
    29041 gaa
//
LOCUS       DAJTRC010000048        28915 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.048, whole genome shotgun sequence.
ACCESSION   DAJTRC010000048 DAJTRC010000000
VERSION     DAJTRC010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28915)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28915)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28915
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.048"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO357_002224"
     CDS             complement(<1..2128)
                     /gene="yccS"
                     /locus_tag="OO357_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01666 family membrane protein"
                     /protein_id="HCR5887157.1"
                     /translation="MAFMLSPLLKRYTWNSAWLYYARIFIALCGTTAFPWWLGDVKLT
                     IPLTLGMVAAALTDLDDRLAGRLRNLIITLFCFFIASASVELLFPWPWLFAIGLTLST
                     SGFILLGGLGQRYATIAFGALLIAIYTMLGTSLYEHWYQQPMYLLAGAVWYNVLTLIG
                     HLLFPVRPLQDNLARCYEQLARYLELKSRMFDPDIEDESQAPLYDLALANGQLMATLN
                     QTKLSLLTRLRGDRGQRGTRRTLHYYFVAQDIHERASSSHIQYQTLREHFRHSDVLFR
                     FQRLMSMQGQACQQLSRCILLRQSYQHDPHFERAFTHIDAALERIRDNGAPADLLKTL
                     GFLLNNLRAIDAQLATIESEQAQTLPRNNDENELADDSPHGLSDIWLRLSRHFTPESA
                     LFRHAVRMSLVLCFGYAIIQITGMHHGYWILLTSLFVCQPNYNATRHRLKLRIIGTLV
                     GIAIGIPVLWFVPSLEGQLVLLVITGVLFFAFRNVQYAHATMFITLLVLLCFNLLGEG
                     FEVALPRVIDTLIGCAIAWAAVSYIWPDWKFRNLPRMLERATEANCRYLDAILEQYHQ
                     GRDNRLAYRIARRDAHNRDAELASVVSNMSSEPNVTPQIREAAFRLLCLNHTFTSYIS
                     ALGAHREQLTNPEILAFLDDAVCYVDDALHHQPADEERVNQALAGLKQRMQQLEPRAD
                     SKEPLVVQQVGLLIALLPEIGRLQRQI"
     gene            complement(2138..2584)
                     /locus_tag="OO357_002225"
     CDS             complement(2138..2584)
                     /locus_tag="OO357_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HCR5887158.1"
                     /translation="MRTVLNILNFVLGGFATTLGWLLATLVSIVLIFTLPLTRSCWEI
                     TKLSLVPYGNEAIHVDELNPAGKNVLLNTGGTVLNIFWLIFFGWWLCLMHIATGIAQC
                     ISIIGIPVGIANFKIAAIALWPVGRRVVSVETAQAAREANARRRFE"
     gene            2707..4761
                     /gene="helD"
                     /locus_tag="OO357_002226"
     CDS             2707..4761
                     /gene="helD"
                     /locus_tag="OO357_002226"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706885.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase IV"
                     /protein_id="HCR5887159.1"
                     /translation="MELKATTLGKRLAQHPYDRAVILNAGIKVSGDRHEYLIPFNQLL
                     AIHCKRGLVWGELEFVLPDEKVVRLHGTEWGETQRFYHHLDAHWRRWSGEMSEIASGV
                     LRQQLDLIATRTGENKWLTREQTSGVQQQIRQALSALPLPVNRLEEFDNCREAWRKCQ
                     AWLKDIESARLQHNQAYTEAMLTEYADFFRQVESSPLNPAQARAVVNGEHSLLVLAGA
                     GSGKTSVLVARAGWLLARGEASPEQILLLAFGRKAAEEMDERIRERLHTEDITARTFH
                     ALALHIIQQGSKKVPIVSKLENDTAARHELFIAEWRKQCSEKKAQAKGWRQWLTEEMQ
                     WSVPEGNFWDDEKLQRRLASRLDRWVSLMRMHGGAQAEMIASAPEEIRDLFSKRIKLM
                     APLLKAWKGALKAENAVDFSGLIHQAIVILEKGRFISPWKHILVDEFQDISPQRAALL
                     AALRKQNSQTTLFAVGDDWQAIYRFSGAQMSLTTAFHENFGEGDRCDLDTTYRFNSRI
                     GEVANRFIQQNPGQLKKPLNSLTNGDKKAVTLLDESQLDALLDKLSGYAKPEERILIL
                     ARYHHMRPASLEKAATRWPKLQIDFMTIHASKGQQADYVIIVGLQEGSGGFPAAARES
                     IMEEALLPPVEDFPDAEERRLMYVALTRARHRVWALFNKENPSPFVEILKNLDVPVAR
                     KP"
     gene            complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO357_002227"
     CDS             complement(4793..5251)
                     /gene="mgsA"
                     /locus_tag="OO357_002227"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HCR5887160.1"
                     /translation="MELTTRTLPARKHIALVAHDHCKQMLMSWVERHQPLLEQHVLYA
                     TGTTGNLISRATGMNVNAMLSGPMGGDQQVGALISEGKIDVLIFFWDPLNAVPHDPDV
                     KALLRLATVWNIPVATNVATADFIIQSPHFNDAVDILIPDYQRYLADRLK"
     gene            complement(5347..6009)
                     /locus_tag="OO357_002228"
     CDS             complement(5347..6009)
                     /locus_tag="OO357_002228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2057 family protein"
                     /protein_id="HCR5887161.1"
                     /translation="MKTGIVTTLIALCLPVSVFATTLRLSTDVDLLVLDGKKVSSSLL
                     RGADSIELDNGPHQLVFRVEKTIHLSNSEERLYISPPLVVSFNTQLINQVNFRLPRLE
                     NEREANHFDAAPRLELLDGDATPIPVKLDILAITSTAKTIDYEVEVERYNKSAKRASL
                     PQFATMMADDSTLLSGVSELDAIPPQSQVLTEQRLKYWFKLADPQTRNTFLQWAEKQP
                     SS"
     gene            6182..6595
                     /locus_tag="OO357_002229"
     CDS             6182..6595
                     /locus_tag="OO357_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HCR5887162.1"
                     /translation="MKETDIAGILTSTHTIALVGASDKPDRPSYRVMKYLLDQGYHVI
                     PVSPKVAGKTLLGQQGYGTLADVPEKVDMVDVFRNSEAAWGVAQEAIAIGAKTLWMQL
                     GVINEQAAVLARDAGLNVVMDRCPAIEIPRLGLAK"
     gene            complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO357_002230"
     CDS             complement(6640..6957)
                     /gene="hspQ"
                     /locus_tag="OO357_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HspQ"
                     /protein_id="HCR5887163.1"
                     /translation="MIASKFGIGQQVRHSLLGYLGVVVDIDPVYSLSEPSPDELAVND
                     ELRAAPWYHVVMEDDNGLPVHTYLAEAQLSSELQDEHPEQPSMDELAQTIRKQLQAPR
                     LRN"
     gene            complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO357_002231"
     CDS             complement(7015..8205)
                     /gene="rlmI"
                     /locus_tag="OO357_002231"
                     /EC_number="2.1.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415487.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytosine(1962)-C(5))-methyltransferase
                     RlmI"
                     /protein_id="HCR5887164.1"
                     /translation="MSVRLVLAKGREKSLLRRHPWVFSGAVARMEGKASLGETIDIVD
                     HQGKWLARGAYSPASQIRARVWTFDPSESIDIAFFSRRLQQAQKWRDWLAQKDGLDSY
                     RLIAGESDGLPGITIDRFGNFLVLQLLSAGAEYQRAALISALQTLYPECAIYDRSDVA
                     VRKKEGMELTQGLVTGELPPALLPIEEHGMKLLVDIQHGHKTGYYLDQRDSRLATRRY
                     VENKRVLNCFSYTGGFAVSALMGGCSQVVSVDTSQEALDIARQNVELNKLDLSKAEFV
                     RDDVFKLLRTYRDRGEKFDVIVMDPPKFVENKSQLMGACRGYKDINMLAIQLLNEGGI
                     LLTFSCSGLMTSDLFQKIIADAAIDAGRDVQFIEQFRQAADHPVIATYPEGLYLKGFA
                     CRVM"
     gene            8300..8578
                     /gene="yccX"
                     /locus_tag="OO357_002232"
     CDS             8300..8578
                     /gene="yccX"
                     /locus_tag="OO357_002232"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HCR5887165.1"
                     /translation="MSKICIIAWIYGRVQGVGFRYTTQYEAKRLGLTGYAKNLDDGSV
                     EVVACGEEGQVEKLMQWLKSGGPRSARVERVLSEPHHPSGELTDFRIR"
     gene            complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO357_002233"
     CDS             complement(8575..8904)
                     /gene="tusE"
                     /locus_tag="OO357_002233"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusE"
                     /protein_id="HCR5887166.1"
                     /translation="MLIFEGKEIETDTEGYLKESSQWSEPLAVVIAENEGISLSPEHW
                     EVVRFVRDFYLEFNTSPAIRMLVKAMANKFGEEKGNSRYLYRLFPKGPAKQATKIAGL
                     PKPVKCI"
     gene            complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO357_002234"
     CDS             complement(8995..9654)
                     /gene="yccA"
                     /locus_tag="OO357_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000375124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease modulator YccA"
                     /protein_id="HCR5887167.1"
                     /translation="MDRIVSSSHDRTSLLSTHKVLRNTYFLLSLTLAFSAITATASTV
                     LMLPSPGLILTLVGMYGLMFLTYKTANKPTGIISAFAFTGFLGYILGPILNTYLSAGM
                     GDVIAMALGGTALVFFCCSAYVLTTRKDMSFLGGMLMAGIVVVLIGMIANIFLQLPAL
                     HLAISAVFILISSGAILFETSNIIHGGETNYIRATVSLYVSLYNIFVSLLSILGFASR
                     D"
     gene            complement(9861..9948)
                     /locus_tag="OO357_002235"
     tRNA            complement(9861..9948)
                     /locus_tag="OO357_002235"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9912..9914),aa:Ser,seq:tga)
     gene            10374..11492
                     /gene="hyaA"
                     /locus_tag="OO357_002236"
     CDS             10374..11492
                     /gene="hyaA"
                     /locus_tag="OO357_002236"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 small subunit"
                     /protein_id="HCR5887168.1"
                     /translation="MNNEETFYQAMRRQGVTRRSFLKYCSLAATSLGLGAGMAPKIAW
                     ALENKPRIPVVWIHGLECTCCTESFIRSAHPLAKDVILSLISLDYDDTLMAAAGTQAE
                     EVFEDIITQYNGKYILAVEGNPPLGEQGMFCISSGRPFIEKLKRAAAGASAIIAWGTC
                     ASWGCVQAARPNPTQATPIDKVITDKPIIKVPGCPPIPDVMSAIITYMVTFDRLPDVD
                     RMGRPLMFYGQRIHDKCYRRAHFDAGEFVQSWDDDAARKGYCLYKMGCKGPTTYNACS
                     STRWNDGVSFPIQSGHGCLGCAENGFWDRGSFYSRVVDIPQMGTHSTADTVGLTALGV
                     VAAAVGVHAVASAVDQRRRHNQQPTETEHQPGNEDKQA"
     gene            11489..13282
                     /gene="hyaB"
                     /locus_tag="OO357_002237"
     CDS             11489..13282
                     /gene="hyaB"
                     /locus_tag="OO357_002237"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase large subunit"
                     /protein_id="HCR5887169.1"
                     /translation="MSTQYETQGYTINNAGRRLVVDPITRIEGHMRCEVNINDQNVIT
                     NAVSCGTMFRGLEIILQGRDPRDAWAFVERICGVCTGVHALASVYAIEDAIGIKVPDN
                     ANIIRNIMLATLWCHDHLVHFYQLAGMDWIDVLDALKADPRKTSELAQSLSSWPKSSP
                     GYFFDVQNRLKKFVEGGQLGIFRNGYWGHPQYKLPPEANLMGFAHYLEALDFQREIVK
                     IHAVFGGKNPHPNWIVGGMPCAINIDESGAVGAVNMERLNLVQSIITRTADFINNVMI
                     PDALAIGQFNKPWSEIGTGLSDKCVLSYGAFPDIANDFGEKSLLMPGGAVINGDFNNV
                     LPVDLVDPQQVQEFVDHAWYRYPNDQVGRHPFDGITDPWYNPGDVKGSDTNIQQLNEQ
                     ERYSWIKAPRWRGNAMEVGPLARTLIAYHKGDAATVESVDRMMSALNLPLSGIQSTLG
                     RILCRAHEAQWAAGKLQYFFDKLMTNLKNGNLATASTEKWEPATWPTECRGVGFTEAP
                     RGALGHWAAIRDGKIDLYQCVVPTTWNASPRDPKGQIGAYEAALMNTKMAIPEQPLEI
                     LRTLHSFDPCLACSTHVLGDDGSELISVQVR"
     gene            13301..14008
                     /gene="hyaC"
                     /locus_tag="OO357_002238"
     CDS             13301..14008
                     /gene="hyaC"
                     /locus_tag="OO357_002238"
                     /EC_number="1.12.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni/Fe-hydrogenase b-type cytochrome subunit"
                     /protein_id="HCR5887170.1"
                     /translation="MQQKSDNVVSHYVFEAPVRIWHWLTVLCMAVLMVTGYFIGKPLP
                     SVSGEATYLFYMGYIRLIHFSAGMIFTVVLLMRIYWAFVGNRYSRELFIVPVWRKSWW
                     QGVWYEIRWYLFLAKRPSADIGHNPIAQAAMFGYFLMSVFMIITGFALYSEHSQYAIF
                     APFRYVVEFFYWTGGNSMDIHSWHRLGMWLIGAFVIGHVYMALREDIMSDDTVISTMV
                     NGYRSHKFGKISNKERS"
     gene            14005..14592
                     /locus_tag="OO357_002239"
     CDS             14005..14592
                     /locus_tag="OO357_002239"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 1 maturation protease"
                     /protein_id="HCR5887171.1"
                     /translation="MSEQHVVVMGLGNLLWADEGFGVRVAERLYAHYHWPEYVEIVDG
                     GTQGLNLLGYVESASHLLILDAIDYGLEPGTLRTYAGERIPAYLSAKKMSLHQNSFSE
                     VLALADIRGHLPAHIALVGLQPAMLDDYGGSLSELAREQLPAAEQSALAQLAAWGSVP
                     QPANESRCLNYDCLSMENYEGVRLRQYRMTQEEQG"
     gene            14589..14987
                     /gene="hyaE"
                     /locus_tag="OO357_002240"
     CDS             14589..14987
                     /gene="hyaE"
                     /locus_tag="OO357_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase-1 operon protein HyaE"
                     /protein_id="HCR5887172.1"
                     /translation="MSNDTPFDALWQRMLARGWTPVSESRLDDWLTQAPDGVVLLSSD
                     PKRTPEVSDNPVMIGELLHEFPDYTWQVAIADLEQSEAIGDRFGAFRFPATLVFTGGN
                     YRGVLNGIHPWAELINLMRGLVEPQQERAS"
     gene            14984..15841
                     /locus_tag="OO357_002241"
     CDS             14984..15841
                     /locus_tag="OO357_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein"
                     /protein_id="HCR5887173.1"
                     /translation="MSETFFHLLGPGTQPNDDSFSMNPLPITCQVNDEPSMAALEQCA
                     HSPQVIALLNELQHQLSERQPPLGEVLAVDLLNLNADDRHFINTLLGEGEVSVRIQQA
                     DDSKSEIQEAIFCGLWRVRRRRGEKLLEDKLEAGCAPLALWQAATQNLLPTDSLLPPP
                     IDGLMNGLPLAHELLAHVRNPDAQPHSINLTQLPISEADRLFLSRLCGPGNIQIRTIG
                     YGESYINATGLRHVWHLRCTDTLKGPLLESYEICPIPEVVLAAPEDLVDSAQRLSEVC
                     QWLAEAAPT"
     gene            15975..17519
                     /gene="appC"
                     /locus_tag="OO357_002242"
     CDS             15975..17519
                     /gene="appC"
                     /locus_tag="OO357_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706901.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit 1"
                     /protein_id="HCR5887174.1"
                     /translation="MWDVIDLSRWQFALTALYHFLFVPLTLGLIFLLAIMETIYVVTG
                     KTIYRDMTRFWGKLFGINFALGVATGLTMEFQFGTNWSFYSNYVGDIFGAPLAMEALM
                     AFFLESTFVGLFFFGWQRLNKYQHLLVTWLVAFGSNLSALWILNANGWMQYPTGAHFD
                     IDTLRMEMTSFSELVFNPVSQVKFVHTVMAGYVTGAMFIMAISAWYLLRGRERDVALR
                     SFAIGSVFGTLAIIGTLQLGDSSAYEVAQVQPVKLAAMEGEWQTEPAPAPFHVVAWPE
                     QDQERNAFAIKIPALLGILATHSLDKPVPGLKNLMAETYPRLQRGRMAWLLMQEISQG
                     NREPHVLQAFRELEGDLGYGMLLSRYAPDMNHVTAAQYQAAMRGAIPQVAPVFWSFRI
                     MVGCGSLLLLVMLIALVQTLRGKIDQHRWVLKMALWSLPLPWIAIEAGWFMTEFGRQP
                     WAIQDILPTYSAHSALTTGQLAFSLIMIVGLYTLFLIAEVYLMQKYARLGPSAMQSEQ
                     PTQQQG"
     gene            17531..18667
                     /gene="appB"
                     /locus_tag="OO357_002243"
     CDS             17531..18667
                     /gene="appB"
                     /locus_tag="OO357_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HCR5887175.1"
                     /translation="MFDYETLRFIWWLLIGVILVVFMISDGFDMGIGCLLPLVARNDD
                     ERRIVINSVGAHWEGNQVWLILAGGALFAAWPRVYAAAFSGFYVAMILVLCSLFFRPL
                     AFDYRGKIADARWRKMWDAGLVIGSLVPPVVFGIAFGNLLLGVPFAFTPQLRVEYLGS
                     FWQLLTPFPLLCGLLSLGMVILQGGVWLQLKTVGVIHLRSQLATKRAALLVMLCFLLA
                     GYWLWVGIDGFVLLAQDANGPSHPLMKLVAVLPGAWMNNFVESPVLWIFPLLGFFCPL
                     LTVMAIYRGRPGWGFLMASLMQFGVIFTAGITLFPFVMPSSVSPISSLTLWDSTSSQL
                     TLSIMLVIVLIFLPIVLLYTLWSYYKMWGRMTTETLRRNKNELY"
     gene            18679..18771
                     /gene="cbdX"
                     /locus_tag="OO357_002244"
     CDS             18679..18771
                     /gene="cbdX"
                     /locus_tag="OO357_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome bd-II oxidase subunit CbdX"
                     /protein_id="HCR5887176.1"
                     /translation="MWYLLWFVGILLMCSLSTLVLVWLDPRLKS"
     gene            18851..20149
                     /gene="appA"
                     /locus_tag="OO357_002245"
     CDS             18851..20149
                     /gene="appA"
                     /locus_tag="OO357_002245"
                     /EC_number="3.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706903.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acid phosphatase/4-phytase"
                     /protein_id="HCR5887177.1"
                     /translation="MKAILIPFLSLLIPLTPQSAFAQSEPELKLESMVIVSRHGVRAP
                     TKATQLMQDVTPDAWPTWPVKLGWLTPRGGELIAYLGHYQRQRLVADGLLAKKGCPQS
                     AQVAIIADVDERTRKTGEAFAAGLAPDCAITVHTQADTSSPDPLFNPLKTGVCQLDNA
                     NVTDAILCRAGGSIADFTGHRQTVFRELERVLNFPQSNLCLNREKQDESCSLTQALPS
                     ELKVSADNVSLTGAVSLASMLTEIFLLQQAQGMPEPGWGRITDSHQWNTLLSLHNAQF
                     YLLQRTPEVARSRATPLLDLIMAALTPHPPQKQAYGVTLPTSVLFIAGHDTNLANLGG
                     ALELNWTLPGQPDNTPPGGELVFERWRRLSDNSQWIQVSLVFQTLQQMRDKTPLSLNT
                     PPGEVKLTLAGCEERNAQGMCSLAGFTQIVNEARIPACSL"
     gene            complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OO357_002246"
                     /pseudo
     CDS             complement(20264..22430)
                     /gene="etk"
                     /locus_tag="OO357_002246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415501.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein kinase"
     gene            complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OO357_002247"
                     /gene_synonym="yccY"
     CDS             complement(22450..22896)
                     /gene="etp"
                     /locus_tag="OO357_002247"
                     /gene_synonym="yccY"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309165.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-tyrosine-phosphatase Etp"
                     /protein_id="HCR5887178.1"
                     /translation="MAQLKFNSILVVCTGNICRSPIGERLLRKRLPGVKVKSAGVHGL
                     VKHPADATAADVAANHGVSLEGHAGRKLTAEMARNYDLILAMESEHIAQVTAIAPEVR
                     GKTMLFGQWLEQKEIPDPYRKSQDAFEHVYSMLERASQEWAKRLSR"
     gene            complement(22884..24023)
                     /locus_tag="OO357_002248"
     CDS             complement(22884..24023)
                     /locus_tag="OO357_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide export protein"
                     /protein_id="HCR5887179.1"
                     /translation="MKKNIFKFSVLTLAVLSLTACTLVPGQNLSTSNKDVIELPDNQY
                     DLDKMVNIYPVTPGLIDQLRAKPIMSQANPELEQQIANYEYRIGIGDVLMVTVWDHPE
                     LTTPAGQYRSASDTGNWVNADGAIFYPYIGRLKVAGKTLTQVRNEITARLDSVIESPQ
                     VDVSVAAFRSQKAYVTGEVSKSGQQPITNIPLTIMDAINAAGGLTADADWRNVVLTQN
                     GVKTKVNLYALMQRGDLRQNKLLHPGDILFIPRNDDLKVFVMGEVGKQSTLKMDRSGM
                     TLAEALGNAEGMNQDVADATGIFVIRATQNKQNGKIANIYQLNAKDASAMILGTEFQL
                     EPYDIVYVTTAPLARWNRVISLLVPTISGVHDLTETSRWIQTWPN"
     gene            complement(24069..26165)
                     /locus_tag="OO357_002249"
     CDS             complement(24069..26165)
                     /locus_tag="OO357_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbH domain-containing protein"
                     /protein_id="HCR5887180.1"
                     /translation="MKKNSYLLSCLAIAVSSACHAEVLTYPDPLGSSQSDFGGTGLLQ
                     MPNARIAPEGEFSVNYRDNDQYRFYSTSVALFPWLEGTIRYTDVRTRKYSQWEDFSGD
                     QSYKDKSFDFKLRLWEEGYWLPQVAFGKRDIAGTGLFDGEYLVASKQAGPFDFTLGMA
                     WGYAGNAGNITNPFCRVSDKYCHRAESHDAGDISFSDIFRGPASIFGGIEYQTPWNPL
                     RLKLEYDGNNYQNDFAGKLPQASHFNVGAVYRAASWADLNLSYERGNTLMFGFTLRTN
                     FNDLRPALRDTPKPAWQPAPESEGLQYTTVANQLTALKYNAGFDAPEIQLRDKTLYMS
                     GQQYKYRDSREAVDRANRILVNNLPQGVEKISVTQKREHMAMVTTETDVASLRKQLAG
                     TAPGKSEQLQQQRVEAEDLSAFGRCYRIREDRFSYSFNPTLSQSLGGPEDFYMFQLGL
                     MSSARYWFTDHLLLDDGIFTNIYNNYDKFKSSLLPADSTLPRVRTHIRDYVRNDVYLN
                     NLQANYFADLGNGFYGQVYGGYLETMYAGVGSELLYRPLDASWALGVDVNYVKQRDWD
                     NMMRFTDYSTPTGFVTAYWNPPTLNGVLMKLSVGQYLAKDKGATIDVAKLFDSGVAVG
                     VWAAISNVSKDDYGEGGFSKGFYISIPFDLMTIGPNRNRAVVSWTPLTRDGGQMLSRK
                     YQLDPMTAEREVPVGQ"
     gene            complement(26165..26911)
                     /locus_tag="OO357_002250"
     CDS             complement(26165..26911)
                     /locus_tag="OO357_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsule biosynthesis GfcC family protein"
                     /protein_id="HCR5887181.1"
                     /translation="MNKLQSYFIASVLYVMTPHAFAQGTVTIYLPSEQQTLSVGPVEN
                     VVQLVTQPQLRDRLWWPGALLTDSAAKAKALKDYQHVMAQLASWEAEADDDVAATIKS
                     VRQQLLNLNITGRLPVKLDPDFVRVDENSNPPLVGDYTLYTVQRPVTITLLGAVSGAG
                     QLPWQAGRSVTDYLQDHPRLAGADKNNVMVITPEGETVVAPVALWNKRHVEPPPGSQL
                     WLGFSAHVLPEKYADLNDQIVSVLTQRVPD"
     gene            complement(26908..27552)
                     /locus_tag="OO357_002251"
     CDS             complement(26908..27552)
                     /locus_tag="OO357_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjbF family lipoprotein"
                     /protein_id="HCR5887182.1"
                     /translation="MRPLILSIFALFLAGCTHSQQSMVDTFRASLFDNQDITVAAQQI
                     QALPYSTMYLRLNEGQRIFVVLGYIEQEQSKWLSQDNAMLVTHNGRLLKTVKLNNNLL
                     EVTNPGRAPLRNALAIKDGSRWTRDILWSEDNHFRSATLSSTFSFAGLETLNIAGHNV
                     LCNVWQEEVTSTLPEKQWQNTFWVDSATGQVRQSRQMLGAGVIPVEMTFLKPAP"
     gene            complement(27659..27964)
                     /locus_tag="OO357_002252"
     CDS             complement(27659..27964)
                     /locus_tag="OO357_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309170.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887183.1"
                     /translation="MKHKLSAILMAFMLTTPAAFAAPEAANGTEATTGTTGTTTTTTG
                     ATTTAATTSGVAAGAVGTATVVGVATAVGVATLAVVAANDSGDGGSHNTSTTTSTTR"
     gene            complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OO357_002253"
     CDS             complement(28406..28618)
                     /gene="cspH"
                     /locus_tag="OO357_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock-like protein CspH"
                     /protein_id="HCR5887184.1"
                     /translation="MSRKMTGIVKTFDRKSGKGFIIPSDGRKEVQVHISAFTPRDAEV
                     LIPGLRVEFCRVNGLRGPTAANVYLS"
BASE COUNT         7004 a         7320 c         7600 g         6991 t
ORIGIN      
        1 taatctggcg ttgcagacga ccaatctctg gcagtaatgc aatcaataac ccaacttgtt
       61 gtacgaccag aggttctttg ctatctgccc gtggttcaag ttgctgcatc cgctgtttca
      121 ggccagctaa tgcctgattg acgcgttctt catcagcagg ttgatgatgt aacgcgtcat
      181 caacatagca aactgcgtca tcaagaaacg ccagaatttc aggattagtt aactgctccc
      241 ggtgagcacc gagggctgag atatagctgg taaacgtatg gttaaggcac agcaagcgaa
      301 acgcggcttc gcgaatttgc ggggtaacgt tcggttcgct ggacatattt gataccaccg
      361 acgccagttc agcatcacgg ttgtgtgcat cgcggcgggc aatacgatac gccagacggt
      421 tatcacgccc ctgatggtat tgctccagta tggcatcgag atagcgacag ttggcctctg
      481 tggcgcgttc gagcattcgc ggcagattgc gaaacttcca gtcaggccag atgtagctca
      541 ctgccgccca cgcaatggca caaccaatca gcgtatcgat tacgcgaggt aacgctactt
      601 caaaaccttc acccagtaag ttaaaacaca gtagcaccaa aagtgtgatg aacatcgttg
      661 catgagcgta ttgcacgtta cggaaggcaa aaaagagcac gccggtaata accagcagca
      721 ccagctgccc ttccagtgat ggcacaaacc acagcacagg aatgccaatc gcgataccta
      781 ccagcgtacc aataatcctt aacttcaggc ggtggcgcgt ggcgttatag tttggctggc
      841 agacaaacaa acttgtcagc aagatccaat acccgtgatg cattccggtt atttgaatga
      901 tggcgtagcc gaagcacaac accagcgaca ttcttaccgc atgacggaag agggcggatt
      961 ccggcgtgaa gtgacggcta agacgcagcc agatatcact caatccgtgc gggctgtcat
     1021 cagcgagctc attttcgtca ttattacggg gtagtgtctg ggcctgttct gattcaattg
     1081 ttgccagttg ggcatcaatg gcgcgtaaat tgttcagcaa aaatcccagt gttttgagta
     1141 aatcggcggg tgcgccgtta tcgcgtatcc gctccagcgc agcatcaata tgcgtaaaag
     1201 cgcgctcaaa atgcggatca tgttgataag actgacgcaa caaaatacag cgtgacagtt
     1261 gctggcacgc ctggccctgc atcgacatca gccgctgaaa acggaacagc acgtcgctgt
     1321 ggcgaaaatg ttcacgcaat gtttgatact gaatatgaga agagctggca cgctcgtgaa
     1381 tatcctgtgc gacaaagtaa taatgcagcg tgcgacgcgt tccccgttga ccacgatcgc
     1441 cacgtaagcg ggtcagcagc gagagtttcg tctgattcaa tgtcgccatc agctgaccgt
     1501 tggcgagagc caaatcgtac agcggtgcct ggctttcatc ttcaatatca ggatcaaaca
     1561 tgcgcgactt gagctcaaga taacgcgcca gttgttcata gcaacgcgcc aggttgtcct
     1621 gcagcgggcg gaccgggaac agcagatgac caataagtgt caggacgttg taccagacgg
     1681 caccggccag cagatacatc ggctgctgat accagtgctc atacagtgat gttcccaaca
     1741 tagtgtaaat ggcgatcagc aatgcaccga aggcaattgt tgcatagcgt tgacccagac
     1801 cgccgagcaa aatgaagccg ctggtagaga gcgttaagcc aatcgcaaat agccagggcc
     1861 agggaaacag caattctact gaggccgagg cgataaaaaa gcagaacagc gtaatgatga
     1921 ggttacgcaa acgtcccgcc agtcggtcat cgagatcggt cagcgccgct gccaccatcc
     1981 ccagcgttag cggaatcgtc agttttacat cacccagcca ccacggaaac gctgtggttc
     2041 cacaaagcgc aataaaaata cgcgcgtaat acagccaggc gctgttccag gtatagcgtt
     2101 tgagcaaagg acttagcata aaggccatat tagtggatta ttcaaaacga cgacgtgcat
     2161 tggcttcacg cgcagcttgc gctgtttcta ccgataccac gcgacgacca accggccata
     2221 gtgcaatagc ggcaatttta aagttcgcaa tgccgacagg aatgccaatg attgaaatac
     2281 attgtgcgat gcccgttgca atgtgcatca ggcataacca ccagccaaag aaaatcagcc
     2341 agaaaatatt caataccgta ccgccagtat tcagcaacac atttttgcca gccgggttca
     2401 gttcatcgac atgaatagct tcattgccat aaggcaccag agacagttta gtgatctccc
     2461 agcaggatcg tgtcagcggt aaggtaaaaa tcagcacaat actgaccaga gtcgccaaca
     2521 gccagcccag agtggtggca aatccgccaa gcacaaagtt cagaatgttc aaaacggtac
     2581 gcataaaacc tcgctttact gtggttttca gtaatgggcg gcaattgtaa cgtttttttg
     2641 ggctggagca cgttttctct gacggttaca ctgataagaa ataatttcgt gtggatctac
     2701 agagtcatgg aactgaaagc gacaacgctt ggaaaacgtc tggcacagca cccttacgat
     2761 cgggcggtga tcctcaatgc cgggattaaa gtctccggcg atcgccacga ataccttatt
     2821 cctttcaatc aattactggc gattcactgt aagcgcggtc tggtatgggg cgagctggaa
     2881 tttgtactgc cggacgaaaa agtggtgcgt ctgcacggca cagaatgggg cgagacgcag
     2941 cgtttttacc atcatcttga tgctcactgg cggcggtgga gtggcgagat gagcgaaatt
     3001 gcgtctggtg ttttacgcca gcaactggat ttgattgcca cgcgcactgg agaaaataaa
     3061 tggctgacgc gtgagcaaac ctctggcgtt cagcaacaaa tccgccaggc tttgtcggcg
     3121 ttgccgttgc cggttaaccg actggaagaa ttcgataact gccgtgaggc gtggcgtaaa
     3181 tgtcaggctt ggttgaaaga tattgaaagc gctcggttgc agcataacca ggcgtatacc
     3241 gaagccatgc ttaccgagta tgcggatttt ttccgccagg tcgagtcttc accgctgaat
     3301 ccggcgcagg cccgggcagt cgttaatggc gagcattctc tgttagtgct ggctggtgca
     3361 ggaagcggaa aaacgtcggt gctggtggcc cgtgcaggct ggttgctggc gcgtggtgaa
     3421 gcgtcccctg agcaaatttt attgctggcg tttggtcgca aagccgctga agagatggac
     3481 gagcggattc gcgaacggct acataccgaa gacattaccg cacgcacgtt tcatgcgttg
     3541 gcgctgcata ttattcagca gggcagcaaa aaagttccga tagtcagcaa actggaaaat
     3601 gataccgctg cccgtcatga actctttatt gctgagtggc gcaagcaatg cagcgaaaag
     3661 aaagcgcagg cgaagggctg gcggcaatgg ctgacggaag aaatgcagtg gtcagtgcca
     3721 gaaggtaact tctgggatga tgaaaaatta cagcgtcgcc ttgcctctcg cctcgatcgc
     3781 tgggtaagtc tgatgcggat gcacggtggt gcacaggcag aaatgattgc cagtgcaccc
     3841 gaagagattc gcgatctgtt cagtaaacgt atcaagttga tggccccgtt attaaaagcc
     3901 tggaaaggtg cgctgaaggc agaaaacgct gtcgattttt cgggccttat tcatcaggcg
     3961 attgtgattc tggagaaagg ccgctttatc agcccgtgga agcatattct ggttgatgaa
     4021 tttcaggata tctcgccgca gcgcgcagcg ttgttagcgg cattacgcaa gcaaaacagt
     4081 cagacgacgt tgttcgccgt tggtgatgac tggcaggcga tttaccgatt cagcggtgcg
     4141 caaatgtcgc tcaccaccgc tttccatgaa aactttggtg aaggcgatcg ctgtgattta
     4201 gacacgactt accgttttaa cagtcgtatc ggtgaggtgg caaaccggtt tattcagcag
     4261 aacccaggcc agctgaaaaa gccgctaaac agcttaacca atggagacaa aaaagccgtc
     4321 acgttattgg atgagagtca acttgacgct ttgctggata agctctctgg ttatgccaaa
     4381 ccggaagagc gcattctgat cctggcgcgt taccatcaca tgaggcctgc cagcctggaa
     4441 aaagcggcaa cacgctggcc gaagttgcaa atcgacttta tgaccattca tgccagcaaa
     4501 gggcaacagg cggattacgt catcatcgtt ggcttgcagg aggggagtgg tggttttccg
     4561 gctgcggcgc gggagtcgat tatggaagag gcgctactgc caccggttga ggatttcccg
     4621 gacgctgaag aacggcgttt aatgtacgtg gcgctgaccc gggcacgcca tcgggtatgg
     4681 gcactgttta acaaagagaa tccctctccc tttgtggaaa tactgaaaaa tctggatgtg
     4741 ccggtggcga gaaaaccgta agaaacaggt ggcgtttgcc acctgtgcaa tattacttca
     4801 gacggtcagc gagataacgc tgataatcgg ggataagaat atcgaccgcg tcgttgaaat
     4861 gcggcgactg gataatgaag tctgccgttg ccacgttggt ggcgaccgga atgttccata
     4921 ccgtcgccag acgcagcaag gctttcacgt caggatcgtg cggcacggca tttagtggat
     4981 cccagaagaa tatcaataca tcaattttcc cttctgagat caatgcgcca acctgctggt
     5041 caccccccat tgggccactc aacatcgcgt tgacgttcat gccggtcgcg cgggaaatca
     5101 agttaccggt agtgcctgtt gcatacagta cgtgttgttc cagtaacggt tgatgccgtt
     5161 ccacccagct catcagcatt tgtttgcagt gatcgtgtgc caccagcgca atatgtttcc
     5221 gcgcaggtaa agtgcgagtc gtcagttcca taatgtacat ccgtagttaa ctttcctaca
     5281 gattactgta agcacttatc gctgcaagat aaagaccgaa aaagcctgcg cacaggcaca
     5341 aaaatctcag gaagatggtt gtttttccgc ccattgcagg aaagtatttc gcgtttgtgg
     5401 gtcagccagt ttaaaccaat acttcagccg ttgttctgtg agcacctgag actgcggtgg
     5461 aatagcatcc agctcggaaa cacccgaaag cagcgtactg tcatctgcca tcatcgtggc
     5521 aaattgcggt agtgaagcgc gtttcgcgga tttgttatag cgttcaacct cgacctcata
     5581 atcaatcgtt tttgccgttg aggtaatggc gagaatatcc agctttaccg gaatcggcgt
     5641 cgcatcgcca tccaacaatt caaggcgcgg cgcggcatca aaatggttag cttcccgctc
     5701 attctccagg cgaggcaggc gaaaattgac ctggttgatg agctgggtat taaaactgac
     5761 caccagcggt ggggagatat acagccgttc ttcactattg gaaagatgaa tcgtcttctc
     5821 aacgcgaaac actaactgat gcggtccatt gtccagttca atgctgtccg caccgcgcag
     5881 cagagagctg gaaaccttct tgccgtcgag taccagaaga tcgacatcgg ttgaaagccg
     5941 cagcgtggtg gcaaaaacgg ataccggcaa acataatgca atcaaggtgg tcacgatgcc
     6001 ggttttcata ggtagctcct gtcaaaagac cgcccgaata ataatgtgtc aaaatttttc
     6061 tttaatcata cttactaaca tatagacata tttcccgctt ttgctctcat tcattcgtat
     6121 tagctgcatg gttggcatgt cggctttgtc gtacactttg ttaaacagtc tggaggaaat
     6181 aatgaaagaa accgatattg caggcatttt aacgtccact cacaccattg cgctggtggg
     6241 ggcaagcgac aaacccgatc gccccagcta tcgcgtgatg aaatatctac tcgaccaggg
     6301 gtatcacgtc attccggttt cgccaaaagt tgccggcaaa acgctgctgg gccagcaggg
     6361 gtatggcacg ctggcagacg ttccggaaaa ggtcgatatg gtcgatgttt tccgtaattc
     6421 agaagcggcg tggggcgtgg cacaggaagc tattgccatt ggcgcgaaaa ccctctggat
     6481 gcaactgggg gtgattaatg agcaagcggc agtactggca cgggatgccg ggttaaatgt
     6541 ggtgatggat cgctgcccgg ctattgagat ccctcgcctg ggtctggcca aataaaaaat
     6601 ccccggaagg caaaaacctt ccggggattt gttcagggat tagttacgca gacgcggggc
     6661 ctggagttgt ttgcggatgg tctgcgccag ctcatccatg gatggctgtt caggatgttc
     6721 atcctgcaat tcgctactta gctgggcttc agccaggtag gtatgaaccg gtaggccgtt
     6781 atcgtcctcc atcaccacgt gataccacgg agcggcgcga agctcgtcat tcaccgccag
     6841 ctcatcgggc gacggttcag agagcgaata aaccgggtcg atatccacga ccactccgag
     6901 ataacctaac agggaatggc ggacctgctg gccgataccg aatttgctgg caatcatagt
     6961 cacctcccgg gaaatcttta cctttgatat aagggtaaaa ttccacattt caagttacat
     7021 gacgcgacag gcaaaccctt tcagatatag cccttccggg taggtagcga tcaccggatg
     7081 atcggctgcc tgacggaact gctctataaa ttgtacatca cgaccggcat caattgcggc
     7141 atccgcgatg attttctgaa ataaatcgct ggtcatcaga ccggaacagg agaaagtcag
     7201 gagaataccg ccttcattca gcaactgaat cgccagcatg ttgatgtctt tataaccacg
     7261 acacgcgccc atcaactggc ttttattctc aacaaacttc ggcgggtcca tcacgataac
     7321 gtcaaatttt tcaccgcgat cgcgataagt acgcagcaat ttaaagacat catcacggac
     7381 aaactcagcc ttgctcagat ccagtttgtt cagctcaacg ttctgccgtg caatatccag
     7441 cgcttcctgg gaggtatcaa cgctgacaac ctggctgcaa ccgcccatca gtgccgatac
     7501 ggcgaaacca ccggtatagg agaaacagtt cagcacacgt ttattttcaa cgtagcggcg
     7561 ggtagccagg cggctgtctc gctggtccag atagtagccc gttttgtgtc cgtgctgaat
     7621 atccaccagc agcttcatgc cgtgttcttc aatcggcagc agggcaggcg gcaactcgcc
     7681 ggtgacgagg ccctgggtca gctccatccc ttctttttta cgtaccgcaa catcgctgcg
     7741 atcgtaaatc gcacattccg ggtacagcgt ttgcagggca ctaattaatg ccgcgcgctg
     7801 atattctgcg ccagcactca gcagttgcag caccagaaaa ttaccgaaac gatcgatagt
     7861 aatacccggc aggccatcag attctccggc gattaaacga tagctgtcga ggccatcttt
     7921 ttgcgccagc cagtcacgcc atttttgtgc ttgttgcaaa cggcgggaaa aaaaagcaat
     7981 gtcgatagac tcagacgggt caaacgtcca gacgcgcgcc cggatttgcg aagctggcga
     8041 ataagcgccg cgtgctaacc attttccctg atgatcaaca atatcgatgg tttcaccgag
     8101 gctggcttta ccttccatgc gggcaacggc cccggaaaag acccacggat ggcgacgaag
     8161 taatgatttt tcgcgccctt tggctaacac taaacgtaca ctcataattt acttttctgt
     8221 cgatgcaaag aaatgggcgt cattgtccgg agttttccat ggatttgcaa cgcgtccatt
     8281 aaggataagg agagcagcaa tgtcgaaaat ctgcataatt gcctggattt acgggcgagt
     8341 tcagggcgta ggatttcgct acaccacaca gtacgaagcg aaaagactgg gcttaaccgg
     8401 gtacgccaaa aatcttgacg atggcagcgt ggaagtggtt gcctgcggtg aagaagggca
     8461 ggtagaaaaa ttaatgcagt ggttaaagag tggcggcccg cgttctgcgc gtgtagaacg
     8521 ggtgctcagc gagccgcatc atccctcggg ggaattaacc gattttcgta tccgttaaat
     8581 acattttacc ggttttggca ggccagcaat tttggtggct tgcttagccg ggcctttcgg
     8641 gaacagtcgg tataaatagc ggctgttacc tttttcttca ccaaatttat tcgccatcgc
     8701 ttttaccagc atacgaatcg ccggagacgt attaaattcc aggtagaagt cacgtacaaa
     8761 acgcaccact tcccagtgtt ctggcgacag cgaaatccct tcgttctctg caatcaccac
     8821 tgccagcggt tcactccact ggctgctttc tttgagatag ccttcggtat ccgtttctat
     8881 ctctttacct tcgaagatca gcataattat tactacctta atcagactgg cggcagtgta
     8941 acaaaaaaca aagccccgca taaagcgagg ctatgaaagt gttagcgggt gagattaatc
     9001 gcggctggcg aagcccagaa tgctcagtag gctgacgaag atgttgtaca gcgaaacata
     9061 caggctaacc gtggcacgaa tatagttcgt ttcaccgcca tgaatgatgt tgctggtttc
     9121 aaacaagata gcgccagagg agatcagaat gaagaccgcg ctgatcgcca gatgcagagc
     9181 aggcagttgc aggaagatat tagcaatcat accaatcagt accaccacaa tacccgccat
     9241 cagcataccg ccgaggaacg acatatcttt gcgggtggtc agcacatatg cagagcagca
     9301 gaagaacact aacgccgttc cgcccagtgc catagcgatt acgtcaccca ttccggcaga
     9361 cagataggtg ttcagaattg gtccgaggat ataacccaga aaaccggtaa aggcgaatgc
     9421 ggagataatc ccggtcggct tattcgccgt tttataggtc aggaacatca aaccatacat
     9481 acccaccaga gtcagaatca gacccggaga tggcagcatc agcaccgtac tggcagttgc
     9541 ggtaatcgcc gaaaaggcca gcgtcaggct cagcagaaaa taggtattac gcagcacctt
     9601 atgggtgcta agcagtgatg tacggtcatg tgaagaacta acaatacgat ccattgagtc
     9661 actctctatg acagatgtaa ttaattaagc agcataatga taatgcgtaa gggcacccag
     9721 aagttttacc catctttacg catttgatct ggaacaggtt taacagcgga ttatcaggtc
     9781 attaagcaaa tataacgccc tgagaattcc gacaggcaaa agaaaaaggg ggtagcgttt
     9841 agctaacccc ttatcttatt tggcggaagc gcagagattc gaactctgga accctttcgg
     9901 gtcgccggtt ttcaagaccg gtgccttcaa ccgctcggcc acacttccgg aatgacgcgc
     9961 actataaaca tcccgatgcg gcgtgtaaac ccctaatttg tttgtttgcc tgaaaaacag
    10021 ccaaaagtgc attgatagcg tgaaataaca gcagattgat catttcatca ccatgaattc
    10081 cttctctttt actcgtttag caaccggcta aacatcccca tcgcccggct aaagaaaaat
    10141 aggtccattt ttatcgctaa aagataaatc cacacagttt gtattgtttt gtgcaaaagt
    10201 ttcactacgc tttattaaca atactttctg gcgacgtgtg ccagtgcaga aggatgagct
    10261 ttcgttttca gcatctcacg tgaagcgatg gtttgccttg ctacagggac gtcgcttgcc
    10321 gaccataagc gcccggtgtc ctgccggtgt tgctaggaga agagacgtgc gatatgaata
    10381 acgaggaaac attttaccag gccatgcggc gtcagggcgt tacccggcgc agctttctca
    10441 aatattgtag tctggctgcc acgtcgctgg gattaggcgc gggaatggca ccaaagattg
    10501 cctgggcgct ggagaacaaa ccgcgcattc cggtggtatg gatccacggt ctggaatgta
    10561 cctgctgtac cgaatctttt atccgctccg ctcacccact ggcgaaggac gtcatccttt
    10621 ccctgatttc cctcgattac gacgataccc tgatggctgc cgccggaacc caggcggaag
    10681 aagtctttga agacatcatc acgcaatata atggcaaata tatcctcgca gtagaaggta
    10741 atccgccgct gggcgagcag gggatgttct gtatcagcag cggtcgaccg tttattgaga
    10801 aactcaaacg tgccgctgcc ggagccagcg cgattatcgc ctggggaacc tgcgcgtcct
    10861 ggggctgcgt gcaggccgcg cgacctaatc cgacacaggc aacgcctatc gacaaagtca
    10921 tcaccgacaa acccattatc aaagtacctg gctgcccgcc gatcccggat gtgatgagcg
    10981 ccatcattac ttacatggtg acctttgatc gcttgccaga tgtcgacaga atgggtcgtc
    11041 cactgatgtt ttatggtcag cgtatccacg ataaatgcta tcgccgcgct cacttcgacg
    11101 ccggagagtt cgtccagagt tgggatgatg acgctgcccg caaaggttat tgcctgtaca
    11161 aaatgggctg caaagggcct accacctata acgcctgttc ctccacacgc tggaatgatg
    11221 gcgtttcttt cccaatccag tctggtcacg gctgcctggg ctgtgcggaa aatggtttct
    11281 gggatcgcgg ttcgttctac agccgcgtgg tcgatattcc gcaaatgggt actcattcca
    11341 ccgccgatac cgtcggttta accgcgcttg gcgtggtggc agcagctgtt ggtgtgcacg
    11401 cagttgccag cgccgttgac caacgcagac gtcataacca gcaacctaca gaaaccgaac
    11461 atcagccagg caatgaggat aaacaggcat gagcactcag tacgaaactc agggatacac
    11521 catcaataat gccggacgcc gcctggtggt cgacccgatt acgcgcatcg aaggccacat
    11581 gcgctgtgaa gtgaatatta acgatcagaa tgtgatcacc aatgccgtct cctgcggcac
    11641 catgtttcgc gggctggaga tcatcctgca agggcgcgac ccgcgcgatg cgtgggcgtt
    11701 cgttgaacgt atctgcggcg tctgtactgg cgtacacgcc ctggcttcgg tttacgccat
    11761 cgaagatgcc atcggtatta aagtgccgga caacgccaat atcatccgca acattatgct
    11821 ggcaacgctc tggtgccacg atcatctggt gcacttctat cagcttgccg ggatggactg
    11881 gatcgacgtg ttagatgcgc tgaaagccga cccgcgaaaa acctccgaac tggcgcaaag
    11941 tctctcctct tggccgaaat catcccctgg ctatttcttc gacgtacaaa atcgcctgaa
    12001 gaaattcgtt gaaggcgggc agttggggat cttccgcaat ggctactggg ggcacccgca
    12061 gtacaaactg ccgccagaag ccaacctgat gggctttgcc cactatctcg aagctctcga
    12121 tttccaacgt gaaattgtca aaattcacgc ggtctttggc ggtaaaaacc cgcatccaaa
    12181 ctggattgtc ggtgggatgc cttgcgccat taacattgac gaaagcggcg cggtcggggc
    12241 ggtcaatatg gaacgcctga acctggtgca gtcgattatc acccgcacgg cagacttcat
    12301 taacaacgtg atgatccccg acgccttagc catcggtcag ttcaacaaac cgtggagcga
    12361 aatcggcacg ggtctttctg ataaatgtgt cctcagctac ggcgcattcc cggatattgc
    12421 caacgacttt ggtgagaaaa gtctgctgat gcctggcggc gcggtgatta acggcgactt
    12481 caacaatgtg ctgccagtgg atttggttga tccgcagcag gtgcaggagt ttgtcgatca
    12541 cgcctggtat cgttatccca acgatcaggt cgggcgtcat ccattcgatg gcattaccga
    12601 cccgtggtac aaccccggcg atgtcaaagg cagcgatacc aacattcagc agctgaatga
    12661 acaggaacgc tactcgtgga tcaaagcgcc acgctggcgc ggtaacgcga tggaagtggg
    12721 gccgctggca cgcacgttaa tcgcttatca caaaggcgat gctgcgaccg ttgagtcggt
    12781 cgatcgcatg atgtcggcgt tgaacctgcc gctttccggt atccagtcaa cgttgggccg
    12841 cattttgtgc cgcgcgcacg aagcgcagtg ggccgcaggt aagttgcagt atttcttcga
    12901 caagctgatg accaacctga aaaacggcaa tctcgccact gcttccactg aaaaatggga
    12961 acctgcaacc tggccgacag agtgccgtgg cgtcggcttt accgaagcgc cgcgcggagc
    13021 gttaggccac tgggccgcca ttcgcgatgg caagattgat ctctaccagt gcgtggtgcc
    13081 gaccacctgg aacgccagtc cgcgcgatcc caaagggcag attggcgctt atgaagcggc
    13141 gctgatgaac accaaaatgg cgatccccga gcaaccgctg gagatactgc gtactctgca
    13201 cagctttgat ccgtgcctcg cctgttcaac tcatgtgctg ggcgacgacg gtagcgagct
    13261 gatctccgtg caggtgcgtt aacagcgaag gagaatcatc atgcaacaga aaagcgacaa
    13321 cgttgtcagc cactatgtct ttgaagcacc agttcgtatc tggcactggc tgacggtgtt
    13381 atgcatggcg gtgttgatgg tcaccggtta ctttatcggc aagccgctac cttccgtcag
    13441 tggcgaggcg acgtatctgt tctatatggg ttacatcagg ttaattcact tcagcgccgg
    13501 gatgattttt accgtggtct tgctgatgcg gatctactgg gcttttgttg gcaatcgtta
    13561 ctcccgcgag ctgtttatcg tgccggtatg gcgtaaaagc tggtggcagg gcgtgtggta
    13621 tgaaatccgc tggtatctgt ttctggcaaa acgtccgagt gccgatatag gccataaccc
    13681 catcgcccag gcggcgatgt tcggctattt cctgatgtcg gtctttatga tcatcaccgg
    13741 ttttgcgctg tacagcgaac acagccagta cgctattttt gcgccgttcc gttatgtggt
    13801 ggaatttttc tactggacgg gtggcaactc aatggacatt cacagctggc atcggctggg
    13861 gatgtggctg attggggcgt ttgtgatcgg tcatgtctac atggcgctgc gtgaagacat
    13921 catgtccgac gacacggtga tctccaccat ggtcaacggc taccgtagcc acaaatttgg
    13981 caaaataagt aacaaggagc gttcatgagc gagcaacacg tggtggtcat ggggctgggc
    14041 aacctgctgt gggccgatga aggcttcggc gtgcgggtgg cggaacggct gtatgcccat
    14101 taccactggc ccgagtatgt ggagattgtc gatggcggta ctcagggact gaacttgctg
    14161 gggtatgtcg aaagcgccag ccatctgttg attctcgatg ccattgacta cgggctggaa
    14221 cctggaacgc tgcgaaccta tgccggagaa cgcattccgg cctatctcag cgcgaagaaa
    14281 atgagcctgc atcagaacag tttctccgaa gtgctggcgc tggcagatat ccgcggacat
    14341 ctgccagcac atattgccct cgtcgggctg caacccgcga tgctcgacga ctacggcggc
    14401 agcctgagcg aactggcacg ggagcaactg cccgctgcgg aacaatcggc gctggcgcag
    14461 ttggctgcgt ggggaagtgt gccgcaaccg gctaatgaat cgcgttgtct caattatgac
    14521 tgtctgtcga tggaaaatta cgaaggcgtt cgcttgcgcc agtaccggat gacacaggag
    14581 gagcagggat gagcaacgac acgccatttg atgcgttgtg gcaacgaatg ctggcgcgcg
    14641 gctggacgcc ggtcagtgaa tcccgtcttg acgactggct tacgcaagcg ccagacggcg
    14701 tggtgttatt aagcagtgac ccgaaacgca cgccagaggt cagcgataat ccggtaatga
    14761 ttggcgaatt actgcacgag tttcccgact atacatggca ggtggcgatt gctgaccttg
    14821 agcagagcga agccatcggc gatcgctttg gcgcctttcg ctttcctgcc actttagtgt
    14881 ttaccggcgg aaactatcgc ggcgtgctga atggtattca cccgtgggcg gaactgataa
    14941 acctgatgcg cgggcttgtc gaaccgcagc aggagcgtgc ctcatgagcg aaactttttt
    15001 ccatctgctg gggccaggaa cgcaaccgaa cgatgacagt ttcagcatga atccactgcc
    15061 gatcacctgt caggtgaatg atgaaccgag tatggcggcc ttggagcaat gtgctcacag
    15121 cccgcaggtg attgcgctgt taaacgagtt acaacatcaa ctaagcgaac gccagccgcc
    15181 gttgggcgag gtgctggcag tcgatctgtt aaatctcaac gccgacgatc gtcactttat
    15241 caatacgctt ctcggggaag gggaagtgtc agtgcgcatt cagcaggctg acgacagtaa
    15301 aagtgaaata caggaggcga tcttctgcgg attatggcgg gtgcgcagac gtcgcggcga
    15361 aaagttgctg gaggacaaac tggaggctgg ctgcgcgccg ctggcgttgt ggcaggcggc
    15421 aacgcaaaat ctcttgccga cagattcgct gttaccgccg cccattgatg gcctgatgaa
    15481 tggcctaccg ttggcgcatg agttactggc acatgtacgt aaccccgacg cgcagccgca
    15541 cagcattaat ctgacgcaat tacccatcag cgaggctgat cggctttttc tctcgcgtct
    15601 ctgtgggccg ggaaatattc agattcgtac cattggctat ggcgagagct atatcaacgc
    15661 cacggggtta cgccatgtct ggcatttacg ctgtacggac accttaaaag gcccgttact
    15721 ggaaagttat gaaatctgcc caataccgga agtggtgctg gcagcgccag aagatttggt
    15781 cgactctgcg cagcggctta gcgaggtatg tcagtggctg gcggaagctg caccgacgta
    15841 aaaagacggt aagtatcgct ttcagtctta tgaatatcgc aatcggcgaa tacctctggt
    15901 cgtagagttt caggataaag agggagatct aacattatcg ggttattttt ctctcttcgc
    15961 ctacaggagt gcgcatgtgg gatgtcattg atttatcgcg ctggcagttt gctctgaccg
    16021 cgctgtatca ctttttattt gtacccctta ccctggggct gatttttttg ctggctatca
    16081 tggaaaccat ttacgtggtt accggcaaaa caatctaccg cgatatgacg cgcttctggg
    16141 gtaagctctt cggtatcaat tttgctcttg gcgtggctac cggcctgacc atggagtttc
    16201 agtttggtac taactggtca ttctattcca actatgtggg cgatattttt ggcgcaccac
    16261 tggcgatgga agcattaatg gccttcttcc tcgaatccac ctttgtcggg ctgttcttct
    16321 tcggctggca acggctgaat aaataccagc acctgctggt gacgtggctg gtggcgttcg
    16381 gttcaaatct ctctgcattg tggatattga atgccaacgg ttggatgcaa tacccgaccg
    16441 gtgcgcattt tgatatcgac accctgcgta tggagatgac cagcttcagc gagctggtct
    16501 ttaacccggt cagccaggtg aaatttgtgc acaccgtcat ggcgggctac gtgaccgggg
    16561 ccatgtttat tatggcgatc agcgcctggt atttactgcg cggacgggag cgcgatgtcg
    16621 cattacgctc gtttgccatc ggttccgtct tcggtactct ggcgattatc ggtaccctgc
    16681 aactcggaga cagttctgcg tatgaagtcg cgcaagtaca accggtaaaa ctggcggcga
    16741 tggaagggga gtggcaaacg gaacctgctc ctgcaccgtt ccatgtggtt gcctggccgg
    16801 aacaggatca agagcgtaac gcctttgcca tcaaaattcc cgcgctgcta gggatcctcg
    16861 ccactcactc attagataaa cccgtgccgg gtctgaagaa tttgatggct gaaacctacc
    16921 cacgcttgca acgcggacgt atggcctggc tgttaatgca ggaaatatct caaggcaatc
    16981 gtgagccgca tgtgttgcag gcattccggg agctggaagg cgaccttggt tacggcatgt
    17041 tgctctcccg ctatgcgccg gatatgaatc atgtcacagc cgcacagtac caggcggcga
    17101 tgcgtggcgc gatacctcag gttgcgccgg tattctggag tttccgcatc atggtgggct
    17161 gtggttccct gctgctactg gtgatgctga ttgcgcttgt ccagacgctg cgtggcaaaa
    17221 tcgaccagca tcgctgggtg ctgaaaatgg cgctctggag tttgccgttg ccgtggattg
    17281 cgattgaagc cgggtggttt atgaccgagt ttggtcgtca gccgtgggcg atacaggaca
    17341 tcttaccgac atactccgcg cactccgctt taaccacagg acaactggct ttctcactga
    17401 tcatgatcgt agggctttac accctgttct taatcgccga agtctacctg atgcagaaat
    17461 atgcccgtct ggggccgagc gcgatgcaga gtgaacaacc gacgcagcaa caggggtaaa
    17521 ggagaaaatc atgtttgatt atgaaacatt gcgcttcatc tggtggctgc tgattggcgt
    17581 gatcctggtg gtctttatga tctccgacgg atttgacatg gggatcggct gtttgctgcc
    17641 gctggtggcg cgtaatgatg atgaacgccg gatagtgatt aatagcgttg gtgcgcactg
    17701 ggaaggcaat caggtctggt tgatcctcgc aggtggggca ttatttgcag cctggcccag
    17761 agtgtatgca gcggcgtttt ccggctttta tgtggcgatg atcctggtgc tgtgctcgct
    17821 gttcttccgt ccgctggcct ttgattatcg cggaaaaatt gccgatgcac gctggcgtaa
    17881 aatgtgggat gccggtctgg tcatcggcag tctggtgccg ccggtagtct tcggtatcgc
    17941 cttcggcaac ttgttgctcg gcgtgccgtt tgccttcaca ccgcaattac gcgtggagta
    18001 tctcggcagc ttctggcaac tgctgacgcc attcccttta ttgtgtggtt tgctcagcct
    18061 tgggatggtg attttgcaag gtggcgtctg gttacaactg aaaactgttg gtgtgattca
    18121 tctgcgttca cagctggcga ctaaacgcgc tgcactgttg gtgatgctgt gctttttgct
    18181 ggcaggttac tggctgtggg tcggtattga tggctttgta ctgctcgccc aggatgctaa
    18241 cggtccttcc catccgttaa tgaaactggt ggcagtgcta cctggtgcct ggatgaataa
    18301 tttcgtcgaa tcgcccgttt tgtggatctt cccgctgctg gggttcttct gcccattgct
    18361 gacggtgatg gcgatttatc gtggtcgccc gggttgggga tttttgatgg catcattgat
    18421 gcaatttggc gtgattttca cggcaggcat cacgctgttc ccctttgtca tgccgtcaag
    18481 tgtgagtcca atctccagcc tgacgttgtg ggacagcact tccagtcagc tgacgctgag
    18541 cattatgttg gtaatcgtgc tgatattttt gcccattgtg ttgctctaca ctctctggag
    18601 ctactacaaa atgtggggac gcatgacaac agaaactctc cgccgtaaca aaaacgagtt
    18661 gtactaagga gcaggaacat gtggtattta ctttggttcg tcggcatttt gttgatgtgt
    18721 tcgctctcca cccttgtgtt ggtatggctg gacccgcgtc tgaaaagtta acgaacgtag
    18781 gcctgatgcg gcgcattagc atcgcatcag gcaatcaata atgttagata tgaaaagcgg
    18841 aaacatatcg atgaaagcga tcttaatccc atttttatct cttctgattc cgttaacccc
    18901 gcaatctgca ttcgctcaga gtgagccgga gctgaagctg gaaagtatgg tgattgtcag
    18961 tcgtcatggt gtgcgtgctc caaccaaggc cacgcaactg atgcaggatg tcaccccaga
    19021 cgcatggcca acctggccgg taaaactggg ttggctgaca ccgcgcggtg gtgagctaat
    19081 cgcctatctc ggacattacc aacgccagcg tctggtagcc gacggattgc tggcgaaaaa
    19141 gggctgcccg cagtctgctc aggtcgcgat tattgctgat gtcgacgagc gtacccgtaa
    19201 aacaggcgaa gccttcgccg ccgggctggc acctgactgt gcaataaccg tacataccca
    19261 ggcagatacg tccagtcccg atccgttatt taatcctcta aaaactggtg tttgccaact
    19321 ggataacgcg aacgtgactg acgcgatcct ctgcagggca ggagggtcaa ttgctgactt
    19381 taccgggcat cggcaaacgg tgtttcgcga actggaacgg gtgcttaatt tcccgcaatc
    19441 aaacttgtgc cttaaccgtg agaaacagga cgaaagctgt tcattaacgc aggcattacc
    19501 atcggaactc aaggtgagcg ccgacaatgt ctcattaacc ggtgcggtaa gcctcgcatc
    19561 aatgctgacg gagatatttc tcctgcaaca ggcacaagga atgccggagc cggggtgggg
    19621 aaggatcacc gattcacacc agtggaacac cttgctaagt ttgcataacg cgcaatttta
    19681 tttactacaa cgcacgccgg aggttgcccg cagtcgcgcc accccgttat tggatttgat
    19741 catggcagcg ttgacgcccc atccaccgca aaaacaggcg tatggtgtga cattacccac
    19801 ttcagtgctg tttattgccg gacacgatac taatctggca aatctcggcg gcgcactgga
    19861 gctcaactgg acgcttccag gtcagccgga taacacgccg ccaggtggtg aactggtgtt
    19921 tgaacgctgg cgtcggctaa gcgataacag ccagtggatt caggtttcgc tggtcttcca
    19981 gactttacag cagatgcgtg ataaaacgcc gctgtcatta aatacgccgc ccggagaggt
    20041 gaaactgacc ctggcaggat gtgaagagcg aaatgcgcag ggcatgtgtt cgttggcagg
    20101 ttttacgcaa atcgtgaatg aagcacgcat accggcgtgc agtttgtaat gcataaaaaa
    20161 gagcattcag ttacctgaat gctctgaggc tgatgacaaa cgaagaactg tctaatgcgt
    20221 agaccggaaa aggcgttcac gccgcatccg gtcactttca gttttactct ttctcggagt
    20281 aactataacc gtaatagtta tagccgtaac tgtaagcggt gctggcgcgt ttaatcacac
    20341 cattgaggat tgcgccttta atattaacgc cagcctgttc cagacgctgc attgacaagc
    20401 tcacctcttt ggcggtgttc aagccaaaac gcgcaaccag caggctggtg ccgacagagc
    20461 gtcccacgac cgcagcattg ctcaccgcca gcatcggcgg cgtatcgaca atcaccagat
    20521 cgtaatggtc gttcgcccat tccagtaatt gacgcatccg atcgcgcatc agcagttcag
    20581 acgggttagg tggcacctga ccgcgagtaa tcacatcaaa gtctcctttg ccaaaatgct
    20641 ggatcacttt gttgagctca tctttacctg ccagatattc cgacaagcca tgttcattac
    20701 tcacggtaaa caggttatgc gaataaccac ggcgtaagtc ggcatcaata aataacactt
    20761 tttgatcgga ctgggcgatc accgctgcca gagttgaact gacaaacgtt ttaccactgt
    20821 ctggcgtcgc accggtgatc atcagaatgt tattctcagt ctccatcata gcgaaatgca
    20881 gactggttcg tagcgcacgt acggcttcca cagcagaatc cgccgggtta tccaccgcca
    20941 ggaaggggat atttttagta cgatggcgct gctgattaga aaataaattt ttcttacgca
    21001 gacgggtgcg tttatccagc cactcggaca ttgggatagt ggaataaacg ctgatgccgt
    21061 gctcttccag ttgttccggg gcttctacac cacgacgcaa catcgcacgc gccagcacgg
    21121 cactcaccga aataaacagg ccaagaataa aaccaagcac cacattcaac gcttttttcg
    21181 gtttcactgg ctgcggctga gtgactgccg ggtcgataat ccgcacgtta ccaatggcac
    21241 tggatttcga aatactcaac tcctgctggc ggttaagtaa ttgcagatat accgcacggc
    21301 ccgcttctac gtcacgactt aaacgcaaca cttcctgttg ggtggaaggc attgccgata
    21361 cccgcttatt caggcgtttg cgttcttgct ccagcgtctg gcgtttttcc agcagcgcac
    21421 gataagttgg gtgatctttc ttatacagct gggagatctc tgcctcgcgg aaggtcagct
    21481 cattgagttg attatcaacg ttcacaatct gctcaagaac ggctttggct tccaggttaa
    21541 ggtcaaccga atcgcgctgc tggcgataaa cgttgagttt ttcttccgcc tggtccagct
    21601 cgctgcgcac ttcaggtaac tggcgctgta agaattcaag gctttgtgaa tcctgcgccg
    21661 cctggcgagc gatattctgt tgcaaatagt tgttagcgat gctgttcaga atacgagtaa
    21721 tcaactgggg atcatcacca gtcatggtaa gttccagcat cccgctttct ttactgcgtt
    21781 cgctaacggt aaaggtttcc tgcaatgcgt taatcgcttc cagttcggta cgctggctca
    21841 ggacaaactg tgttcctggt ttggccttaa tgtccgcgat agtcagcgca acgccatctt
    21901 tttccagacg ctggccgacc ataccattga cggtgaactc ttcaccttcc agtgtatagt
    21961 ggccgttttc cccaaccgtg agtgtcagtt gctgatcctg accattcagt tgtggaatat
    22021 gcatccagct gatcgccagc tcacctagtt tttctttggt taatctcgcc cagccgcgac
    22081 ccacaatcgg aaaatacttc tgctcaacta tgtcgcgcag attcagttca gcaatggttt
    22141 taccgagaat catgcgcgat tgcagcagtt ggatctccgg tgcagactcg ggcgatgagt
    22201 tagggatcat atcgctcagg ccgctgagaa tggcgttgcc ctgtttttgc tcaacctgga
    22261 ccagagtatc tgcctgataa attggtgtgc ttaacagcga tcagcgtgaa taacgcggtc
    22321 acgctgataa taaacttacg gtgatcccat aactcgccga ccagacgaag cagatcgatc
    22381 tcattttcct gagtgctgcc tggtggcgta ttcatatttt tagttgtcat cgtattttat
    22441 aaactcaaat taccggctga ggcgcttcgc ccattcctga ctggcgcgct ccaacatact
    22501 gtagacatgt tcaaatgcgt cctgactttt acgataggga tccgggatct ctttctgttc
    22561 cagccattgc ccaaacagca ttgtcttccc gcggacctcg ggagcaattg cggtaacctg
    22621 agcaatatgt tccgactcca ttgccagaat cagatcgtaa tttcgtgcca tctcagcagt
    22681 gagcttacgt ccggcatgtc cttcaagaga tacgccatga ttagcggcaa catctgccgc
    22741 tgtcgcatct gcagggtgtt ttaccagacc atgaacacct gccgatttga cttttacgcc
    22801 cggcagacgt ttacgcagca agcgctcgcc aatgggcgaa cggcaaatgt tgccggtaca
    22861 aaccactagg attgagttaa attttagttg ggccatgttt gaatccaccg tgaagtctct
    22921 gtcaggtcat gcaccccgga aatcgtcggt accagcagag aaatcacacg gttccaacga
    22981 gccagcggcg cggtagtgac ataaacaatg tcgtaaggtt caagctggaa ttccgttccg
    23041 agtatcattg ccgatgcgtc cttcgcattc agctgataaa tattggcgat tttgccattc
    23101 tgcttgtttt gcgttgcacg aataacaaaa atccccgtcg catcagcgac atcctgattc
    23161 ataccctcag cgttacccag cgcttctgcc agggtcatac cgctgcggtc cattttcagc
    23221 gtgctctgtt tacccacttc acccatcacg aagactttca gatcatcgtt gcgcggaata
    23281 aataaaatat cgcccgggtg cagcagtttg ttctggcgca agtcgccacg ctgcatcagc
    23341 gcatagaggt tcactttggt ctttacgcca ttctgggtga gcaccacgtt gcgccagtct
    23401 gcatctgcgg tcagaccgcc cgcggcgtta atcgcatcca tgatggtcag aggaatattg
    23461 gtaatcggct gctgacccga tttgctgact tcgccggtga cgtacgcttt ttgcgatcgg
    23521 aacgccgcga cgctgacatc gacttgcggg ctttcaatga ctgaatcgag acgcgctgta
    23581 atctcgttgc gtacctgggt aagtgttttc ccggccactt tcaggcgacc aatatagggg
    23641 tagaaaatcg caccatcggc gttaacccag ttgccggtat cactggcgct acggtattgc
    23701 cctgcggggg tggttagttc cgggtgatcc cagaccgtaa ccatcaacac atcaccaatc
    23761 ccgatacgat attcatagtt tgcgatttgc tgctccagct ccgggttagc ctgcgacata
    23821 atcggtttgg cgcgcagctg atcaatcaat cctggcgtga cgggatagat attgaccatt
    23881 ttgtccaggt catactgatt gtcaggtagt tcaatgacat ccttgttact ggtcgataaa
    23941 ttctgaccag gaacaagggt acatgctgtc aaagacagaa cagccaatgt cagtaccgaa
    24001 aacttaaaaa tgttcttttt cattggttta tatttatcag tcaatgtaat gttatgaatt
    24061 acgtcaggtt attgtccaac cggtacttct cgctctgccg tcattggatc gagctggtat
    24121 ttgcgtgaca gcatttgtcc accatcacgc gtcaatggtg tccacgaaac caccgcgcgg
    24181 ttgcggttag gtccaatggt catcaagtcg aacggaatcg agatataaaa acctttacta
    24241 aagccgcctt cgccgtagtc atctttagat acgttactga ttgccgccca tacccctacc
    24301 gccacgccgc tgtcaaagag tttggcgacg tcgatcgttg cgcctttatc ttttgccaga
    24361 tattgcccaa cgctaagttt catcagtacg ccattgagcg tcggcgggtt ccagtaagcc
    24421 gtcacgaaac ctgtgggcgt ggaataatcg gtgaagcgca tcatgttgtc ccagtcacgc
    24481 tgcttcacgt agttaacgtc cacacccagc gcccagctgg catctagcgg gcgataaaga
    24541 agctcggaac cgacaccggc atacatcgtt tccagataac cgccatacac ctggccatag
    24601 aaaccattgc ctaagtcggc aaagtaattc gcttgcaagt tgttgagata aacgtcattg
    24661 cgaacgtaat cacggatatg cgtgcgcacg cggggcaggg tagagtccgc gggtaacagt
    24721 gaagacttaa acttgtcgta gttgttgtaa atattggtga aaataccgtc atcaagcagc
    24781 aggtggtcgg taaaccagta gcgggcgctg gacatcagcc ccagctggaa catatagaaa
    24841 tcttccgggc cgcccagcga ctgtgaaagt gttgggttga aagagtagct aaagcgatct
    24901 tcacgaatac ggtagcaccg accaaaggca gaaagatctt ctgcttcaac acgttgttgt
    24961 tgcagttgct ctgatttacc aggcgctgtg ccggccagct gtttgcgcag gctggctacg
    25021 tcggtttcgg tagtcaccat cgccatatgc tcgcgctttt gcgtcacgct aatcttctca
    25081 acgccttgcg gcaggttatt caccagaatc cggttggcac gatcgaccgc ttcgcgagag
    25141 tcacggtatt tgtattgctg accagacata tacagcgtct tatcgcgtag ctgaatttct
    25201 ggcgcgtcaa agcccgcgtt atacttcagg gcggtaagtt ggtttgctac cgtggtgtac
    25261 tgcaaacctt cagactctgg cgcaggctgc caggccggtt ttggcgtatc gcgtagggca
    25321 gggcgcagat cgttgaaatt ggtccgtaaa gtgaaaccaa acatcaacgt gtttccgcgt
    25381 tcataactga ggttgaggtc tgcccagctg gcagcgcgat aaactgcacc gacgttgaaa
    25441 tggcttgcct gaggcagttt gccagcgaaa tcattctggt aattgttgcc gtcgtattcg
    25501 agtttcagac gcaggggatt ccacggcgtt tgatactcaa tgccgccaaa gatggaagcc
    25561 gggccacgaa agatatcgct aaagctgata tcgcccgcat cgtgagactc tgcgcgatga
    25621 caatatttat cgctcacccg gcaaaacggg ttggtaatat tgcccgcatt accggcgtag
    25681 ccccatgcca tcccgagggt gaaatcaaat ggccccgctt gcttgctggc caccagatat
    25741 tcaccgtcaa acaaacccgt accagcaata tcacgtttac caaacgccac ttgcggtagc
    25801 cagtaacctt cttcccacag gcgaagttta aaatcgaatg atttgtcttt gtatgactga
    25861 tcgccgctga aatcttccca ctggctatat ttgcgtgtgc gcacatccgt ataacgaatg
    25921 gtgccttcca gccatgggaa cagcgccacg gaggtggagt agaaccggta ttgatcgtta
    25981 tcccggtagt tgacgctgaa ttcaccttcc ggtgcgatgc gcgcatttgg catctgcaac
    26041 aatcctgtgc cgccaaagtc tgattgcgac gaacccagcg gatccgggta ggttaatact
    26101 tcagcatgac aggcactgga gacggcaatg gccaggcagc ttaaaagata agaattcttc
    26161 ttcatcagtc aggaacacgt tgcgttaaaa cagacacaat ctggtcattt aaatcagcat
    26221 atttttcggg cagtacatgc gcagaaaagc ccaaccaaag ctggctgcca ggcggtggtt
    26281 ccacatgtct tttattccac agggcaacag gggcgacgac agtctcaccc tcaggcgtaa
    26341 tcaccataac gttattcttg tctgcgcctg caaggcgggg gtgatcttgc agataatcgg
    26401 taacgctacg gcctgcctgc cagggcaatt gcccagcccc agaaaccgca cccagcagag
    26461 taatagtcac cggacgctgt acggtataga gcgtatagtc cccgaccaac gggggattgc
    26521 tgttctcatc cactcgtaca aaatcagggt ccagtttgac cggtaagcga ccagtgatat
    26581 tgagattgag taactgctgg cgcacggatt taatcgttgc cgccacatcg tcgtcagctt
    26641 ctgcttccca ggaagcaagc tgtgccatga catgttggta gtctttaagc gctttagctt
    26701 tcgccgcaga gtcggtcagc aaggcaccag gccaccagag gcgatcgcgc agttgcggtt
    26761 gtgtcaccaa ctggacaaca ttttccacgg ggccaacgga aagtgtctgt tgttcgctag
    26821 gcagataaat agtcaccgtt ccttgcgcaa aggcatgggg tgtcattacg taaagtacgc
    26881 tggcaatgaa atacgactgt aatttattca tggtgcgggt ttaagaaacg tcatttctac
    26941 gggaataacc cctgcgccta acatttgccg actttgacga acctggccag tagccgaatc
    27001 gacccagaat gtgttttgcc actgtttttc tggcagcgtg gaagtcacct cttcctgcca
    27061 gacattacac agcacattgt gacccgcaat attcagcgtc tctaatccag caaaggaaaa
    27121 agtagaactt agggtcgcag agcgaaaatg gttgtcttca ctccagagaa tatcgcgcgt
    27181 ccagcggctg ccatctttta ttgccagcgc gttacgcaga ggggcccgcc cgggatttgt
    27241 cacttccagc agattattat taagtttgac ggtctttaaa agacgtccat tgtgggtaac
    27301 cagcatggcg ttatcctggg ataaccattt gctttgttct tgttctatat atcccagtac
    27361 cacaaagatt cgttgccctt cattaaggcg taaatacatc gtggaataag gcaacgcctg
    27421 gatctgctga gccgctacag tgatatcctg attatcgaaa aggctggcgc gaaatgtatc
    27481 gaccatactt tgctggctgt gcgtacatcc cgcaagaaat agtgcgaaaa tcgataaaat
    27541 aagagggcgc acctttaatc cttgaaaggt taattaaaaa aatgcaaatc tctaaagccc
    27601 ggtacgaata tcgcaaccgg gtattattta acttgaattt cgacgcgttt aagcagaatt
    27661 agcgcgtagt ggacgtggtg gttgaggtgt tatgagaacc gccatcgcca ctgtcatttg
    27721 ctgcaactac tgccaacgtc gccacaccaa cagccgttgc cacaccgaca actgtcgccg
    27781 tgccgacagc gccagcagcc acgccactag tggtagccgc agtcgtggtt gcacctgtag
    27841 tggtcgttgt ggtgccagta gtacccgtcg ttgcctcggt cccgtttgct gcttcagggg
    27901 cagcaaacgc agctggcgtc gtcaacatga aggccataag gattgcagaa agcttgtgtt
    27961 tcataacttt tcctttattc atcgcatgga caatacgtac gcttgagatc aggattgtct
    28021 actttgagta taacatcctt gtgaaatctc cctttcctca taccgctagc actatatact
    28081 gcgggtggga aaggcgcgca gagggaaata taagattgtt aacgatttcc ctctggaaat
    28141 acgacttgtg agcataagat aaatcttaga aaaatacgaa ttaaagataa gaaaattgtt
    28201 acgaaagcta ttaattattg ttggtaatat agtttcaagt ggtactataa ctggctgtta
    28261 aatggcattt ataactatta ggtgcagaga tattcgctta aatggattag tttcttagtg
    28321 gaaaagtaac tgatgttata atgtatggtg ttgactattg aactattcca ataaaaccag
    28381 tcagctttaa acaagcagcg tcatattaag agagataaac atttgccgct gttggtcctc
    28441 gcaggccatt tacgcggcaa aattccacgc gtaatcctgg tataagcact tctgcgtcgc
    28501 ggggagtgaa tgcggaaata tggacctgga cttctttacg accgtcggag gggataatga
    28561 atcctttgcc gcttttgcga tcaaaggttt tgacaattcc tgtcatttta cgggacaaac
    28621 aaattcctta ctgaaaatac tgcgctgcac tatacggggt taataaaata aagccagcga
    28681 tatttaagac cgccggacgg ctaaaataaa atttgcttaa tctcaattat catgccttaa
    28741 tagctgcgtc ggtttgaaag acagacagca tacaaagtag tttactaaag cagttctcat
    28801 tatcaggcat tatccccttc ttttgagtct ctctcctgaa cactaagtgg tttctgtatt
    28861 aaagccctgt ttgccgaaag gcccaaaatg aaggaagtaa aatatgtcta ataaa
//
LOCUS       DAJTRC010000049        28811 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.049, whole genome shotgun sequence.
ACCESSION   DAJTRC010000049 DAJTRC010000000
VERSION     DAJTRC010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28811)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28811)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28811
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.049"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..238
                     /locus_tag="OO357_002254"
     CDS             <1..238
                     /locus_tag="OO357_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5887185.1"
                     /translation="DCKPEEEIDKYHNPLNKIICEETIRLGGSMVHHHGIGKHRVHWS
                     KLEHGSAWALLEGLKKQFDPNGIMNTGTIYPIEK"
     gene            260..1669
                     /locus_tag="OO357_002255"
     CDS             260..1669
                     /locus_tag="OO357_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5887186.1"
                     /translation="MTGRCLFGFSGEKPFLLPDNEGVKMNTSPVRMDDLPLNRFHCRI
                     ATLTFGAHLTDGYVLGVIGYAIIQLTPAMQLTPFMAGMIGGSALLGLFLGSLVLGWIS
                     DHIGRQKIFTFSFLLITLASFLQFFATTPEHLIGLRILIGIGLGGDYSVGHTLLAEFS
                     PRRHRGILLGAFSVVWTVGYVLASIAGHHFISENPEAWRWLLASAALPALLITLLRWG
                     TPESPRWLLCQGRFAEAHAIVHRYFGPHVLLGDEVVTATHKHIKTLFSSRYWRRTAFN
                     SVFFVCLVIPWFVIYTWLPTIAQTIGLEDALTASLMLNALLIVGALLGLVLTHLLAHR
                     KFLLGSFLLLAATLVVMACLPSGSSLTLLLFVLFSTTISAVSNLVGILPAESFPTDIR
                     SLGVGFATAMSRLGAAVSTGLLPWVLAQWGMQVTLLLLATVLLVGFVVTWLWAPETKA
                     LPLVAAGNVGGANEHSVSV"
     gene            1647..2426
                     /locus_tag="OO357_002256"
     CDS             1647..2426
                     /locus_tag="OO357_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HCR5887187.1"
                     /translation="MNILLAFKAEPDAGMLAEKEWQAAAQGNSGPDVSLLRSLLGADE
                     QAAAALLLAQRKNGTPMSLTALSMGDERALHWLRYLMALGFEEAVLLETAADLRFAPE
                     FVARHIAEWQRQNPLDLIITGSQSSEGQNGQTPFLLAEMLGWPCFTQVERFTLDALFI
                     TLEQRTEHGLRCCRVRLPAVIAVRQCGEVALPVPGMRQRMAAGKAEIIRKTVAAEMPA
                     MQCLQLARAEQRRGATLIDGQTVAEKAQKLWQDYLRQRMQP"
     gene            2423..3283
                     /locus_tag="OO357_002257"
     CDS             2423..3283
                     /locus_tag="OO357_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HCR5887188.1"
                     /translation="MNIAIVTINQENAAIASWLAAQDFSGCTLAHWQIEPQPVVAEQV
                     LDAMVEQWQRTPADVVLFPPGTFGDELSTRLAWRLHGASICQVTSLDIPTVSMRKSHW
                     GNALTATLQTEKRPLCLSLARQAGAAKNATLPSGMQQLNIVPGALPDWLVSTEDLKNV
                     SRDPLAEARRVLVVGQGGEADNQEIVMLAEKLGAEVGYSRARVMNGGVDAEKVIGISG
                     HLLAPEVCIVVGASGAAALMAGVRNSKFMVAINHDASAAVFSQADIGVVDDWKVVLEA
                     LVTNIHAEYQ"
     gene            complement(3431..4006)
                     /locus_tag="OO357_002258"
                     /pseudo
     CDS             complement(3431..4006)
                     /locus_tag="OO357_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417248.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate responsive antiterminator"
     gene            complement(4023..4283)
                     /locus_tag="OO357_002259"
     CDS             complement(4023..4283)
                     /locus_tag="OO357_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311649.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5887189.1"
                     /translation="MSVARNLWRVADAPHIVPADSVERQMVQRLINACPVGLFSLTQE
                     GDLRIDYRGCLECGTCRLLCDESTLQQWRYPPSGFGITYRFG"
     gene            complement(4274..5545)
                     /locus_tag="OO357_002260"
     CDS             complement(4274..5545)
                     /locus_tag="OO357_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5887190.1"
                     /translation="MEDDCDIIIIGAGIAGTACALRCARADLSVLLLERAEIPGSKNL
                     SGRRLYTHALAELLPQFHLTAPLERRITHESLSLLTPDGATTFSSLQPGGESWNVLRA
                     RFDPWLVAEAEKEGVECIPGATVDALYEENGRVCGVICGDDILRARYVVLAEGANSVL
                     AERHGLVTRPAGEAMALGIKEVLSLETSAIEERFHLENNEGAALLFSGGICDDLPGGA
                     FLYTNQQTLSLGIVCPLSSLTQSRVPASELLTRFKAHPAVRTLIKNTESLEYGAHLVP
                     EGGLHSMPVQYAGNGWLLVGDALRSCVNTGISVRGMDMALTGAQAAAQTLISACQHRE
                     PQNLFALYHHNVERSLLWDVLQRYQHVPALLQRPGRYRAWPALMQDISRDLWDQGDKP
                     VPPLRQLFWHHLRRHGLWHLAGDVIRSLRCL"
     gene            complement(5623..5985)
                     /gene="queD"
                     /locus_tag="OO357_002261"
     CDS             complement(5623..5985)
                     /gene="queD"
                     /locus_tag="OO357_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase QueD"
                     /protein_id="HCR5887191.1"
                     /translation="MSTTLFKDFTFEAAHRLPHVPEGHKCGRLHGHSFMVRLEITGEV
                     DPHTGWIIDFAELKAAFKPTYERLDHHYLNDIPGLENPTSEVLAKWIWDQVKPVVPLL
                     SAVMVKETCTAGCIYRGE"
     gene            6304..8103
                     /gene="cysJ"
                     /locus_tag="OO357_002262"
     CDS             6304..8103
                     /gene="cysJ"
                     /locus_tag="OO357_002262"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent assimilatory sulfite reductase
                     flavoprotein subunit"
                     /protein_id="HCR5887192.1"
                     /translation="MTTQVPPSALLPLNPEQLARLQAATTDLTPTQLAWVSGYFWGVL
                     NQQPVALAVTPAPAAEMPGITIISASQTGNARRVAEALRDDLLAAKLNVKLVNAGDYK
                     FKQIASEKLLIVVTSTQGEGEPPEEAVALHKFLFSKKAPKLENTAFAVFSLGDSSYEF
                     FCQSGKDFDSKLAELGGERLLDRVDSDVEYQAAASEWRARVVDALKSRAPVAAPSQSV
                     ATGAVNEIHTSPYSKDAPLVASLSVNQKITGRNSEKDVRHIEIDLGDSGLRYQPGDAL
                     GVWYQNDPALVKELVELLWLTGDEPVTVEGKTLPLNEALQWHFELTVNTANIVENYAT
                     LTRSETLLPLVGDKAKLQHYAATTLIVDMVRFSPAQLDAEALINLLRPLTPRLYSIAS
                     SQAEVENEVHVTVGVVRYDVEGRARAGGASSFLADRVEEEGEVRVFIEHNDNFRLPAN
                     LETPVIMIGPGTGIAPFRAFMQQRAADEAPGKNWLFFGNPHFTEDFLYQVEWQRYVKE
                     GVLTRIDLAWSRDQKEKVYVQDKLREQGAELWRWINDGAHIYVCGDANRMAKDVEQAL
                     LEVIAEFGGMDTEAADEFLSELRVERRYQRDVY"
     gene            8103..9815
                     /gene="cysI"
                     /locus_tag="OO357_002263"
     CDS             8103..9815
                     /gene="cysI"
                     /locus_tag="OO357_002263"
                     /EC_number="1.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001290736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="assimilatory sulfite reductase (NADPH)
                     hemoprotein subunit"
                     /protein_id="HCR5887193.1"
                     /translation="MSEKHPGPLVVEGKLTDAERMKLESNYLRGTIAEDLNDGLTGGF
                     KGDNFLLIRFHGMYQQDDRDIRAERAEQKLEPRHAMLLRCRLPGGVITTKQWQAIDKF
                     AGENTIYGSIRLTNRQTFQFHGILKKNVKPVHQMLHSVGLDALATANDMNRNVLCTSN
                     PYESQLHAEAYEWAKKISEHLLPRTRAYAEIWLDQEKVATTDEEPILGQTYLPRKFKT
                     TVVIPPQNDIDLHANDMNFVAIAENGKLVGFNLLVGGGLSIEHGNKKTYARTASEFGY
                     LPLEHTLAVAEAVVTTQRDWGNRTDRKNAKTKYTLERVGVETFKAEVERRAGIKFEPI
                     RPYEFTGRGDRIGWVKGIDDNWHLTLFIENGRILDYPGRPLKTGLLEIAKIHKGDFRI
                     TANQNLIIAGVPESEKAKIEKIAKESGLMNAVTPQRENSMACVSFPTCPLAMAEAERF
                     LPSFIDNIDNLMVKHGVSDEHIVMRVTGCPNGCGRAMLAEVGLVGKAPGRYNLHLGGN
                     RIGTRIPRMYKENITEPEILASLDELIGRWAKEREAGEGFGDFTVRAGIIRPVLDPAR
                     DLWD"
     gene            9889..10623
                     /gene="cysH"
                     /locus_tag="OO357_002264"
     CDS             9889..10623
                     /gene="cysH"
                     /locus_tag="OO357_002264"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
                     /protein_id="HCR5887194.1"
                     /translation="MSKLDLNALNELPKVDRILALAETNAELEKLDAEGRVAWALDNL
                     PGEYVLSSSFGIQAAVSLHLVNQIHPDIPVILTDTGYLFPETYRFIDELTDKLKLNLK
                     VYRATESAAWQEARYGKLWEQGVEGIEKYNDINKVEPMNRALKELNAQTWFAGLRREQ
                     SGSRANLPVLAIQRGVFKVLPIIDWDNRTIYQYLQKHGLKYHPLWDEGYLSVGDTHTT
                     RKWEPGMAEEETRFFGLKRECGLHEG"
     gene            10887..11039
                     /locus_tag="OO357_002265"
     CDS             10887..11039
                     /locus_tag="OO357_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Hok family toxin"
                     /protein_id="HCR5887195.1"
                     /translation="MLTKYALVAIIVLCCTVLGFTLMVGDSLCELSIRERGMEFKAVL
                     AYESKK"
     gene            11233..12324
                     /locus_tag="OO357_002266"
                     /pseudo
     CDS             11233..12324
                     /locus_tag="OO357_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311642.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated endonuclease Cas3''"
     gene            complement(12470..13507)
                     /gene="iap"
                     /locus_tag="OO357_002267"
     CDS             complement(12470..13507)
                     /gene="iap"
                     /locus_tag="OO357_002267"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase isozyme conversion
                     aminopeptidase"
                     /protein_id="HCR5887196.1"
                     /translation="MFSALRHRTAALALGVCFILPVHASSPKPGDFANTQARHIATFF
                     PGRMTGTPAEMLSADYIRQQFQQMGYRSDIRTFNSRYIYTARDNRKNWHNVTGSTVIA
                     AHEGKAPQQIIIMAHLDTYAPLSDADADANLGGLTLQGIDDNAAGLGVMLELAERLKN
                     TPTEYGIRFVATSGEEEGKLGAENLLKRMSDTEKKNTLLVINLDNLIVGDKLYFNSGV
                     KTPEAVRKLTRDRALAIARSHGIAATTNPGLNKNYPKGTGGCNDAEIFDKAGIAVLSV
                     EATNWNLGNKDGYQQRAKTPAFPAGNSWHDVRLDNQQHIDKALPGRIERRCRDVMRIM
                     LPLVKELAKAS"
     gene            13759..14667
                     /gene="cysD"
                     /locus_tag="OO357_002268"
     CDS             13759..14667
                     /gene="cysD"
                     /locus_tag="OO357_002268"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysD"
                     /protein_id="HCR5887197.1"
                     /translation="MAQKRLTHLRQLEAESIHIIREVAAEFSNPVMLYSIGKDSSVML
                     HLARKAFYPGTLPFPLLHVDTGWKFREMYEFRDRTAKAYGCELLVHKNPEGVAMGINP
                     FVHGSAKHTDIMKTEGLKQALNKYGFDAAFGGARRDEEKSRAKERIYSFRDRFHRWDP
                     KNQRPELWHNYNGQINKGESIRVFPLSNWTEQDIWQYIWLENIDIVPLYLAAERPVLE
                     RDGMLMMIDDNRIDLQPGEVIKKRMVRFRTLGCWPLTGAVESNAQTLPEIIEEMLVST
                     TSERQGRVIDRDQAGSMELKKRQGYF"
     gene            14669..16096
                     /gene="cysN"
                     /locus_tag="OO357_002269"
     CDS             14669..16096
                     /gene="cysN"
                     /locus_tag="OO357_002269"
                     /EC_number="2.7.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate adenylyltransferase subunit CysN"
                     /protein_id="HCR5887198.1"
                     /translation="MNTVLAQQIANEGGVEAWMIAQQHKSLLRFLTCGSVDDGKSTLI
                     GRLLHDTRQIYEDQLSSLHNDSKRHGTQGEKLDLALLVDGLQAEREQGITIDVAYRYF
                     STEKRKFIIADTPGHEQYTRNMATGASTCELAILLIDARKGVLDQTRRHSFISTLLGI
                     KHLVVAINKMDLVDYSEETFTRIREDYLTFAGQLPGNLDIRFVPLSALEGDNVASQSE
                     SMPWYSGLTLLEVLETVEIQRVVDAQPMRFPVQYVNRPNLDFRGYAGMLASGRVEVGQ
                     RVKVLPSGVESNVARIVTFDGDREEVFAGEAITLVLTDEIDISRGDLLLAADEALPAV
                     QSASVDVVWMAEQPLSPGQSYDIKIAGKKTRARVDGIRYQVDINNLTQREVENLPLNG
                     IGLVDLTFDEPLVLDRYQQNPVTGGLIFIDRLSNVTVGAGMVHEPVSQATAASSEFSA
                     FELELNALVRRHFPHWGARDLLGDK"
     gene            16096..16701
                     /gene="cysC"
                     /locus_tag="OO357_002270"
     CDS             16096..16701
                     /gene="cysC"
                     /locus_tag="OO357_002270"
                     /EC_number="2.7.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylyl-sulfate kinase"
                     /protein_id="HCR5887199.1"
                     /translation="MALHDENVVWHSHPVTVQQRELHHGHRGVVLWFTGLSGSGKSTV
                     AGALEEALHKLSVSTYLLDGDNVRHGLCSDLGFSDADRKENIRRVGEVANLMVEAGLV
                     VLTAFISPHRAERQMVRERVGEGRFIEVFVDTPLAICEARDPKGLYKKARAGELRNFT
                     GIDSVYEAPESAEIHLNGEQLVTNLVQQLLDLLRQNDIIRS"
     gene            16751..17074
                     /locus_tag="OO357_002271"
     CDS             16751..17074
                     /locus_tag="OO357_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3561 family protein"
                     /protein_id="HCR5887200.1"
                     /translation="MRNSHNITLTNNDSLTEDEETTWSLPGAVVGFISWLFALAMPML
                     IYGSNTLFFFLYTWPFFLALMPVAVVVGIALHSLMDGKLRYSIVFTLVTVGIMFGALF
                     MWLLG"
     gene            17268..17579
                     /gene="ftsB"
                     /locus_tag="OO357_002272"
     CDS             17268..17579
                     /gene="ftsB"
                     /locus_tag="OO357_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsB"
                     /protein_id="HCR5887201.1"
                     /translation="MGKLTLLLLAILVWLQYSLWFGKNGIHDYTRVNDDVAALQATNA
                     KLKARNDQLFAEIDDLNGGQEALEERARNELSMTRPGETFYRLVPDASKRAQSAGQNN
                     R"
     gene            17598..18308
                     /gene="ispD"
                     /locus_tag="OO357_002273"
     CDS             17598..18308
                     /gene="ispD"
                     /locus_tag="OO357_002273"
                     /EC_number="2.7.7.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase"
                     /protein_id="HCR5887202.1"
                     /translation="MATTHLDVCAVVPAAGFGRRMQTECPKQYLSIGNQTILEHSVHA
                     LLAHPRVTRVVIAISPGDSRFAQLPLANHPQITVVDGGDERADSVLAGLKAAGDVQWV
                     LVHDAARPCLHQDDLARLLALSETSRTGGILAAPVRDTMKRAEPGKNAIAHTVDRNGL
                     WHALTPQFFPRELLHDCLTRALNEGATITDEASALEYCGFHPQLVEGRADNIKVTRPE
                     DLALAEFYLTRTIHQENT"
     gene            18308..18787
                     /gene="ispF"
                     /locus_tag="OO357_002274"
     CDS             18308..18787
                     /gene="ispF"
                     /locus_tag="OO357_002274"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HCR5887203.1"
                     /translation="MRIGHGFDVHAFGGEGPIIIGGVRIPYEKGLLAHSDGDVALHAL
                     TDALLGAAALGDIGKLFPDTDPAFKGADSRELLREAWRRIQAKGYTLGNVDVTIIAQA
                     PKMLPHIPQMRVFIAEDLGCHMDDVNVKATTTEKLGFTGRGEGIACEAVALLIKATK"
     gene            18784..19833
                     /gene="truD"
                     /locus_tag="OO357_002275"
     CDS             18784..19833
                     /gene="truD"
                     /locus_tag="OO357_002275"
                     /EC_number="5.4.99.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(13) synthase TruD"
                     /protein_id="HCR5887204.1"
                     /translation="MIEFDNLTYLHGKPQGTGLLKANPEDFVVVEDLGFEPDGEGEHI
                     LVRILKNGCNTRFVADALAKFLKIHSREVSFAGQKDKHAVTEQWLCARVPGKEMPDLS
                     AFQLEGCQVLEYARHKRKLRLGALKGNAFTLVLREVSNRDDVEQRLIDICVKGVPNYF
                     GAQRFGIGGSNLQGALRWAQTNTPVRDRNKRSFWLSAARSALFNQIVAERLKKADVNQ
                     VVDGDALQLAGRGSWFVATTEELAELQRRVNDKELMITAALPGSGEWGTQREALAFEQ
                     AAVAAETELQALLVREKVEAARRAMLLYPQQLSWNWWDDVTVEIRFWLPAGSFATSVV
                     RELINTTGDYAHIAE"
     gene            19814..20575
                     /gene="surE"
                     /locus_tag="OO357_002276"
     CDS             19814..20575
                     /gene="surE"
                     /locus_tag="OO357_002276"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HCR5887205.1"
                     /translation="MRILLSNYDGVHAPGIQTLAKALREFADVQVVAPDRNRSGASNS
                     LTLESSLRTFTFENGDIAVQMGTPTDCVYLGVNALMRPRPDIVVSGINAGPNLGDDVI
                     YSGTVAAAMEGRHLGFPALAVSLDGHKHYDTAAAVTCSILRALCKEPLRTGRILNINV
                     QDLPLDQIKGIRVTRCGTRHPADQVIPQQDPRGNTLYWIGPPGGKCDAGPGTDFAAVD
                     EGYVSITPLHVDLTAHSAQDVVSDWLNSVGVGTQW"
     gene            20569..21195
                     /gene="pcm"
                     /locus_tag="OO357_002277"
     CDS             20569..21195
                     /gene="pcm"
                     /locus_tag="OO357_002277"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HCR5887206.1"
                     /translation="MVSRRVQALLDQLRAQGIQDEQVLNALAAVPREKFVDEAFEQKA
                     WDNIALPIGQGQTISQPYMVARMTELLELTPQSRVLEIGTGSGYQTAILAHLVQHVCS
                     VERIKGLQWQARRRLKNLDLHNVSTRHGDGWQGWQARAPFDAIIVTAAPPEIPTALMT
                     QLDEGGILVLPVGEEHQYLKRVRRRGGEFIIDTVEAVRFVPLVKGELA"
     gene            21335..22474
                     /gene="nlpD"
                     /locus_tag="OO357_002278"
     CDS             21335..22474
                     /gene="nlpD"
                     /locus_tag="OO357_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator NlpD"
                     /protein_id="HCR5887207.1"
                     /translation="MSAGSPKFTVRRIAALSLVSLWLAGCSDTSNPPAPVSSVNGNAP
                     ANTNSGMLITPPPKMGTTSTAQQPQIQPVQQPQIQATQQPQIQPVQPVAQQPVQMENG
                     RIVYNRQYGNIPKGSYSGSTYTVKKGDTLFYIAWITGNDFRDLAQRNNIQAPYALNVG
                     QTLQVGNASGTPITGGNAITQADAAEQGVVIKPAQNSTVAVASQPTITYSESSGEQSA
                     NKMLPNNKPTATTVTAPVTVPTASTTEPTVSSTSTSTPISTWRWPTEGKVIETFGASE
                     GGNKGIDIAGSKGQAIIATADGRVVYAGNALRGYGNLIIIKHNDDYLSAYAHNDTMLV
                     REQQEVKAGQKIATMGSTGTSSTRLHFEIRYKGKSVNPLRYLPQR"
     gene            22537..23529
                     /gene="rpoS"
                     /locus_tag="OO357_002279"
     CDS             22537..23529
                     /gene="rpoS"
                     /locus_tag="OO357_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000081498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoS"
                     /protein_id="HCR5887208.1"
                     /translation="MSQNTLKVHDLNEDAEFDENGVEVFDEKALVEEEPSDNDLAEEE
                     LLSQGATQRVLDATQLYLGEIGYSPLLTAEEEVYFARRALRGDVASRRRMIESNLRLV
                     VKIARRYGNRGLALLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMN
                     QTRTIRLPIHIVKELNVYLRTARELSHKLDHEPSAEEIAEQLDKPVDDVSRMLRLNER
                     ITSVDTPLGGDSEKALLDILADEKENGPEDTTQDDDMKQSIVKWLFELNAKQREVLAR
                     RFGLLGYEAATLEDVGREIGLTRERVRQIQVEGLRRLREILQTQGLNIEALFRK"
     gene            complement(23649..24056)
                     /locus_tag="OO357_002280"
     CDS             complement(23649..24056)
                     /locus_tag="OO357_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HCR5887209.1"
                     /translation="MALRNKAFHQLRQLFQQHTARWQHELPDLTKPQYAVMRAIADKP
                     GIEQVALIEAAVSTKATLAEMLARMENRGLVRREHDVADKRRRFVWLTAEGEKLLAAA
                     IPIGDSVDEEFLGRLSGEEQELFMQLVRKMMST"
     gene            24203..24796
                     /locus_tag="OO357_002281"
     CDS             24203..24796
                     /locus_tag="OO357_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HCR5887210.1"
                     /translation="MKLIVGMTGATGAPLGVALLQALREMPNVETHLVMSKWAKTTIE
                     LETPYSARDVAALADFSHNPADQAAIISSGSFRTDGMIVIPCSMKTLAGIRAGYADGL
                     VGRAADVVLKEGRKLVLVPREMPLSTIHLENMLALSRMGVAMVPPMPAFYNHPETVDD
                     IVHHVVARVLDQFGLEHPHARRWQGLPQARNFSQENE"
     gene            24796..25743
                     /locus_tag="OO357_002282"
                     /pseudo
     CDS             24796..25743
                     /locus_tag="OO357_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311619.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
     gene            complement(26016..26579)
                     /locus_tag="OO357_002283"
     CDS             complement(26016..26579)
                     /locus_tag="OO357_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887211.1"
                     /translation="MRKAPQGSFPADGKEPIIERIFRLVERYPSRNAAARAWGINEGT
                     LKNYYNRRDYAPIPRPYQLKKIAESENVTLEWLMTGEGDVELKDSKEPKKENADNVDA
                     QILMFLSFLKPQEKQQVADVLGRKGAEQLLILLDEHIQELHALVGVRRAIALSLGNLP
                     DEKVREIYALSEAKGDHLNLPQKAASA"
     gene            26744..26995
                     /locus_tag="OO357_002284"
     CDS             26744..26995
                     /locus_tag="OO357_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5887212.1"
                     /translation="MRQREDSDRDWPSLKIQLALKDNGWSSFRELSLHAGYAQDSLKA
                     VVRTPNKKYERVISDAIGVPPEIIWPSRYRNDGFIRKAS"
     gene            26997..>28811
                     /locus_tag="OO357_002285"
     CDS             26997..>28811
                     /locus_tag="OO357_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708540.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5887213.1"
                     /translation="MQSYVTVNDLLGVPGMPATTKGIRQALQRFSRDLGDVSRRREGT
                     KAIEYHIDCLPEITRKALRERYVEQLVATENNVSEVKAVTRKTRNPDAVQAIEAYRGS
                     PQLMEERLNALTENQRWVSEARAALVVEVLKLESAGNPGRLKAINFLVEKARKGELPE
                     RLQQAAVNANAKRGANRTISRDPLYQWVLKYNQSQNAAERLLLLAPGKRDEIKPEEIS
                     WLPEFLAQYRQVNGRPMSEAYEDFVAEWQRRHADEPYMLEVMPSYDVVRYAMKKLPEV
                     VKQKGRVTGSEYRQLEGFTRRDWTAMPVNYVWIGDGHGMKLKCAHPIHGRPFSPEVTF
                     VIDGGTRFVVGWSLDLAENVFAVAGAIQHGIRNHGKPFLYYSDNGSGETADMLDKEVV
                     GILPRLGIKHPTGIAGNPQGRGIIERLNRTLPMRIARRYRTYFGKGADRESLRVLNRD
                     LRSAFNALQQDKPLNDRQKAAMRELPSWAELIEAIREGVEWYNNRPHSELPMKPNGRH
                     YSPTEFRKKRQAEEDTEIEWLSDLELRDMFRPMVERPVRRCEIQWLNNIYYAPELRDE
                     HGRKVLISYDIHDAERITVRRKDGSFICEAIWNGNKRAA"
BASE COUNT         7003 a         7156 c         7816 g         6836 t
ORIGIN      
        1 tgactgtaag ccggaagagg aaatcgacaa gtaccacaat ccgctcaaca aaatcatctg
       61 cgaagaaacc attcgcctcg gcggttcgat ggtgcaccac catggcattg gtaaacatcg
      121 cgttcactgg agcaagctgg aacacggcag cgcgtgggcg ttgctggaag ggctgaaaaa
      181 gcagttcgat cctaacggca ttatgaatac gggtactatc tatccgattg aaaaataatg
      241 tatcaggcag cgttccgcga tgacggggcg ttgccttttc ggcttctcag gcgagaagcc
      301 gttcttatta ccggacaatg aaggggtaaa gatgaacact tcaccggtgc gaatggatga
      361 tttaccgctt aaccgttttc actgtcgaat tgctacgctc actttcggcg cacacctgac
      421 cgacggttat gttctaggcg tcattggtta cgccattatt caacttacgc ccgccatgca
      481 actgacgccg tttatggcgg gaatgatcgg cggctcggcg ctccttggtt tgtttcttgg
      541 cagcctggtt cttgggtgga tctccgacca tattggtcgg caaaaaatct tcaccttcag
      601 ctttttgctg attacgctcg cttcgttctt gcaatttttt gccaccacgc cagagcatct
      661 tattgggctg cgcattttga tcggcattgg tctgggaggc gattactcag taggtcacac
      721 cttgctggct gaattttccc cgcgccgcca tcgcggtatt ttgctgggcg cattcagcgt
      781 ggtgtggacc gtaggctatg tgttggcaag tattgccgga catcacttta tttccgaaaa
      841 cccggaggcc tggcgctggt tgctggcatc ggcagctctg cccgcgttgt tgattacgtt
      901 attacgctgg ggaacgccag aatcgccacg ttggctactg tgccaggggc gttttgcaga
      961 agctcacgct atcgtgcatc gctattttgg tccccatgtt ttactgggcg atgaagtggt
     1021 aacggcgacc cataaacaca tcaaaacctt gttctcttcg cgttactggc ggcgcacggc
     1081 gtttaacagc gtcttctttg tctgcctcgt aatcccatgg tttgtgattt atacctggct
     1141 gccaactatc gcccagacta ttggtctgga agatgcgctg actgccagcc tgatgcttaa
     1201 tgcgttgtta attgtgggcg cgctgctggg attagttctg acgcacctgc tggcacatcg
     1261 caaatttttg ctgggaagtt ttttgctgct ggcggcaacg ctggtagtaa tggcctgttt
     1321 gccttccggc agttcattaa cgctgctgct ttttgttctc ttcagcacca ccatttcggc
     1381 agtcagtaat ctggtgggca ttttgcctgc ggaaagtttt cctactgaca ttcgctcgct
     1441 gggagtcggt tttgccaccg ccatgagtcg acttggcgcg gcggtaagta ctggcctgct
     1501 gccgtgggtg ctggcgcagt ggggaatgca agtcacctta ttgctcctag cgacagtgtt
     1561 gttggttggt tttgttgtga cctggctatg ggcaccagaa actaaagccc tcccgctggt
     1621 ggcggcggga aatgtaggag gtgcgaatga acattctgtt agcgtttaaa gccgaaccgg
     1681 atgccggaat gctggcggaa aaagagtggc aggcggcggc tcagggtaat agcggaccgg
     1741 atgtttcgct actgcgaagt ttactcggtg ctgatgaaca ggccgctgcc gcgctgctgt
     1801 tagcgcagag aaaaaacggt acgccgatgt ctttaaccgc gttgagcatg ggggatgaac
     1861 gggcgttgca ctggctgcgc tatctcatgg ctcttgggtt tgaggaagct gtattgctgg
     1921 agacggcggc agatctgcgc tttgcgccgg aatttgtcgc tcgccatatt gccgaatggc
     1981 agcgtcagaa tccgctggat ctgataatta ccggcagcca aagcagcgaa gggcagaatg
     2041 ggcaaacgcc atttttgctg gcggaaatgc tgggctggcc ctgcttcacc caggtggagc
     2101 gtttcactct cgacgcgctg tttatcaccc tcgaacagcg tactgaacat gggttgcgct
     2161 gttgccgggt tcgcttgcct gcggtgattg ccgtgcgtca gtgcggtgaa gtggccttgc
     2221 cagtaccggg aatgcgccag cgaatggctg cggggaaagc agaaattatc cgtaaaactg
     2281 ttgctgccga aatgccagcg atgcagtgtc tgcaactggc aagagcagag caacgacgcg
     2341 gagcgacgct gattgatggg caaacggtag cagaaaaagc tcaaaagcta tggcaggact
     2401 atttacgcca aaggatgcag ccatgaatat cgcaattgtg acaataaatc aggagaatgc
     2461 cgcaatagcg agctggctgg cggcgcagga tttcagcggt tgtacgctgg cgcactggca
     2521 aatagaacct cagcccgtag tggcggaaca ggtgctggat gcgatggtgg aacagtggca
     2581 gcgaacgcct gcggacgttg ttctctttcc gccgggaacg tttggcgatg aactgtcgac
     2641 ccggctggcc tggcgtttac atggtgcaag catctgtcag gtgacttcgt tagatattcc
     2701 aaccgtaagc atgcgcaaat cccactgggg aaatgcgtta acggcgacgc tgcaaacaga
     2761 aaaacgcccg ttatgccttt ccctggcgcg tcaggcggga gcagcgaaaa acgccacgtt
     2821 gccctctgga atgcagcaac tgaacattgt cccaggtgcg cttcccgact ggctggttag
     2881 cacagaggat ctgaaaaatg ttagccgcga cccattagct gaggccagac gggtgctggt
     2941 ggtgggacag ggcggagagg cagataacca ggaaatcgtt atgctggcag aaaagctggg
     3001 agccgaagtg ggctacagtc gggcgcgagt gatgaacggt ggcgttgatg ctgaaaaagt
     3061 gattggtatt tccggacatc tgctggcacc tgaggtgtgc attgttgttg gcgcttccgg
     3121 cgcagcagcg ttaatggcgg gcgtacgcaa tagcaagttt atggtggcga ttaatcatga
     3181 cgccagcgca gcggtatttt cacaggcaga tattggcgtg gttgatgact ggaaagtggt
     3241 gctggaggca ctggtgacaa acattcacgc agaataccaa taacgtgctg ccggatgatc
     3301 ctgcctacgg ttctggactg gggacctgta ggcctgataa gacgcgttaa gcgtcgcatc
     3361 aggcgctgaa tgtcggatgt ggcgtgcggc caacaaatca aaacaaattc aattggttac
     3421 gattatcccg tcaaagcaat tttttcgcta acgtccagac tccggtattc gtggtggaaa
     3481 gcgccacgac acccgcgtta atcgcattac gcgcatcttc ttcatcgcat accagcccgc
     3541 cagcaatcag cggttggcgg attttttccg tcacccagcc cagcactttg ggcatgcagc
     3601 ctggcaggat ctcaatacaa tccggattcg attgcgcaac ttacttatca atgttgtgaa
     3661 acgaaatcga atcaacaata aacaggtgat gaatacagaa aaaaacttcc gctcttgccg
     3721 cttttagcat cgatgctttg gtgctgataa ttccatccgc ctcggtcacc agtttcaaaa
     3781 actgaatcac cacttcttta tttgacgcgc cttccagcaa atcaacatga ataaaagcat
     3841 atttcccggc gttcttaatc tttttaacga tattactgat ggtgcagata ttgccgtaca
     3901 acacggaaat aaactggcat tcagaatcga tggccagttg caggctggca ttatctttaa
     3961 cggcagcaat caccggattc tggcggagca ggtgtaagag gggcataaat tatccttatt
     4021 atttatccaa agcggtaggt gatgccgaat ccggaaggcg gatagcgcca ctgttgtagt
     4081 gtggattcgt cgcacagcaa acggcaggtg ccacactcca ggcagccgcg atagtcaata
     4141 cgtaagtcac cttcctgtgt gagagaaaaa agacctaccg gacaggcgtt aatcaaccgt
     4201 tgtaccatct gacgctcaac ggagtcagcc ggaacaatgt gcggcgcatc agcaacgcgc
     4261 cagagattac gggctacaga catcgcagac tcctgataac atcgcccgcc agatgccaca
     4321 ggccatgacg acgtaaatga tgccagaata actggcgcag cggtggaaca ggtttatcac
     4381 cctgatccca taaatcgcgg gaaatatcct gcattaacgc aggccacgcc cgatacctgc
     4441 cagggcgttg caaaagcgcc ggaacatgct gataacgttg tagaacatcc catagcaggc
     4501 tgcgctctac gttgtgatga taaagcgcaa acagattttg cggctcgcgg tgctggcagg
     4561 cgcttattag cgtttgtgcc gccgcctgtg cgccagtcag cgccatatcc atgccgcgca
     4621 cggaaattcc ggtattgacg caactgcgca acgcatcgcc caccagcagc cagccgttac
     4681 cggcgtattg caccggcata ctgtgcaagc caccttctgg caccagatgc gcaccatact
     4741 ccagtgattc cgtgtttttg ataagcgtgc gcactgccgg atgcgcttta aagcgagtca
     4801 gcagctcgct tgccggaaca cgactttgcg taagggaaga gagcgggcaa acaatcccta
     4861 acgagagcgt ttgttgatta gtataaagaa atgcgccgcc gggtaagtca tcacagattc
     4921 cgccgctgaa cagcaacgct gcgccttcgt tattctccag atgaaaacgt tcttcaatag
     4981 cggatgtttc cagcgacagc acttctttga tccccaacgc catcgcttcg ccagcaggac
     5041 gagtcactaa cccgtgacgt tcagccagga cgctgttggc accttctgcc agcaccacat
     5101 aacgggcgcg gagaatatcg tcaccacaaa taacgccaca gactctgccg ttttcttcat
     5161 acagtgcatc caccgtcgct ccggggatgc attcgacacc ttctttttcg gcttcggcaa
     5221 ccagccacgg atcgaaccgt gcacgtaata cattccagga ttcaccgccg ggctgtaagc
     5281 tggaaaacgt cgttgcgcca tccggcgtta acagggaaag gctttcgtga gtgatgcgtc
     5341 gttcaagagg cgcggtcaga tgaaattgcg ggaggagttc cgcgagtgca tgggtatata
     5401 accgcctgcc ggaaagattt ttgctgccgg ggatttcagc gcgttcgagt aacaaaacgg
     5461 ataaatccgc tcgcgcgcag cgtaacgcgc aagcggtgcc tgcaataccg gcaccaataa
     5521 taataatgtc gcagtcgtct tccatgtcat gccccagata tccagaacac aacaccctaa
     5581 catagcgtta ctaaagggaa attgaccgcc gacacactct tatcattcgc cgcgatagat
     5641 acaccctgcg gtgcaggttt cttttaccat caccgcactt aacagcggca caacgggttt
     5701 aacctgatcc caaatccatt ttgctaaaac ttcgctggtt ggattttcca gacctggaat
     5761 atcattgaga taatggtgat cgaggcgctc ataagttggc ttaaacgccg ctttcagttc
     5821 agcgaaatcg ataatccagc ccgtatgcgg atcgacttcc ccggtaattt ccagtcgcac
     5881 cataaaggaa tgcccgtgca ggcgaccaca tttatgccct tccgggacat gtggtaagcg
     5941 gtgagcggct tcgaaggtga aatctttaaa taacgtggtg gacatcataa tttctctaca
     6001 ggacgataaa accgccgtag agtaccggaa aggctgtttt ttcgcattat ctaaaacgac
     6061 tttgttgcgc aaaatcgctg atttatctta atgattggct aaattcattt gtttttcatt
     6121 aggttggtta atctattttg ttgttaaaga ctattgctaa aacaggttag tcgatttggt
     6181 tattagttat cgctatcccg tctttaatcc acaccgttca ccccgttaac cttaccttct
     6241 cttctgtttt atgggcgctg acagggcgca gaaacagctt tgcttactgg aacataacga
     6301 cgcatgacga cacaggtccc accttccgcg ttgcttccgt tgaacccgga gcaactggca
     6361 cgccttcagg cggccacgac cgatttaacg cctacccagc ttgcctgggt ttctggctat
     6421 ttctggggcg tgctcaacca gcagcctgtt gcgcttgcag tgacgccagc gcccgccgca
     6481 gaaatgccgg gtataacgat tatctctgct tcgcaaaccg gcaatgcgcg ccgggttgct
     6541 gaagcattac gcgatgattt attagcagca aaactgaacg ttaagctggt gaacgcgggc
     6601 gactataaat tcaaacaaat cgccagcgaa aaactgctca tcgtagtgac gtcaacgcaa
     6661 ggggaagggg aaccgccgga agaagccgtc gcgctgcata agttcctgtt ctccaaaaaa
     6721 gcgccaaagc tggaaaacac cgcgtttgcc gtgtttagcc tcggcgatag ctcttatgaa
     6781 tttttctgcc agtccgggaa agattttgac agcaagctgg cggaactggg gggtgaacgc
     6841 ctgctcgacc gtgtcgattc tgatgttgaa taccaggctg ctgccagcga gtggcgcgcc
     6901 cgcgtggttg atgcgcttaa atcgcgtgcg cctgtcgcgg caccttcgca atccgtcgct
     6961 actggcgcgg taaatgaaat ccacaccagc ccgtacagca aagacgcgcc gctggtggct
     7021 agcctctctg ttaaccagaa aattactggg cgtaactctg aaaaagacgt tcgccatatc
     7081 gaaattgact taggcgactc gggcctgcgt taccagccgg gtgacgcgct gggcgtctgg
     7141 tatcagaacg atccggcact ggtgaaagaa cttgtcgaac tgctgtggct gacaggcgat
     7201 gaacctgtca ctgtcgaggg caaaacgttg cctctgaacg aagcgctaca gtggcacttc
     7261 gaactgaccg tcaacaccgc caacattgtt gagaattacg ccacgcttac ccgcagtgaa
     7321 acactgctgc cgctggtggg cgataaagcg aagttacagc attacgccgc gacgacgctg
     7381 attgttgaca tggtgcgttt ctccccggca cagcttgatg ccgaagcgct aattaatctg
     7441 ctgcgcccgc tgaccccgcg tctctattcc attgcctcct cgcaggcgga agtcgagaac
     7501 gaagtacacg tcaccgttgg tgtggtgcgt tacgacgtgg aaggccgcgc ccgtgccggt
     7561 ggtgcctcca gcttcctcgc tgaccgcgtg gaagaagagg gcgaagtccg cgtatttatc
     7621 gaacataacg ataacttccg cctgcccgct aacctggaaa ccccggtgat tatgattggc
     7681 ccaggcaccg gtattgcgcc gttccgcgcc tttatgcagc aacgcgccgc tgacgaagcg
     7741 ccaggtaaaa actggctgtt ctttggcaat ccgcatttta cggaagactt cctgtatcag
     7801 gtggagtggc agcgctacgt caaagagggt gtgctgacgc ggatcgatct cgcctggtcg
     7861 cgcgaccaaa aagaaaaagt ttacgtacaa gataaactgc gcgaacaggg cgcggagctg
     7921 tggcgctgga tcaatgacgg tgcccacatt tatgtctgcg gcgacgctaa tcgcatggcg
     7981 aaagacgttg agcaggcact tctggaagtg attgccgaat ttggtggcat ggacaccgaa
     8041 gcggcggatg aatttttaag tgagctgcgc gtagagcgcc gttatcagcg agatgtctac
     8101 taatgagcga aaaacatcca gggcctttag tggtcgaagg aaaactgaca gacgccgagc
     8161 gcatgaagct tgaaagcaac tacctgcgcg gcaccattgc ggaagattta aacgacggtc
     8221 tgaccggcgg ctttaagggc gataacttcc tgctgatccg cttccacggc atgtatcagc
     8281 aggatgaccg cgacatccgc gccgaacgtg ctgaacagaa gctggagccg cgccacgcga
     8341 tgctgttgcg ctgccgtctg ccgggtggag ttatcaccac caaacagtgg caggcgatcg
     8401 ataaatttgc cggtgaaaac accatctatg gcagcattcg cctgaccaac cgccagacgt
     8461 ttcagttcca cggcattctg aaaaagaacg tcaaaccggt gcaccagatg ctgcactcgg
     8521 tcggtcttga tgcgctggcg actgctaacg acatgaaccg taacgtactc tgcacctcga
     8581 acccttacga gtcgcagctg cacgcagaag cgtacgagtg ggcgaagaag atttctgaac
     8641 atctgctgcc gcgtacccgc gcgtatgcgg agatctggct cgatcaggaa aaagtcgcca
     8701 ccaccgatga agaaccgatc cttggtcaga cttacctgcc gcgtaagttc aaaaccacgg
     8761 tagtgatccc gccgcagaac gatattgatc tgcacgccaa cgacatgaac ttcgtggcga
     8821 tcgcggaaaa cggcaagctg gtgggcttca acctgctggt gggcggtggg ctttctatcg
     8881 aacacggtaa caagaaaacc tacgcccgca cggcgagtga gtttggctat ctgccgctag
     8941 agcatacgct agcggtggcg gaagccgtcg tgacaactca gcgtgactgg ggtaaccgaa
     9001 ccgatcgtaa aaatgccaaa accaaataca cactggagcg cgtgggggtt gagacgttta
     9061 aagcggaagt ggagcgtcgc gcggggatca aatttgaacc gatccgcccg tatgaattta
     9121 caggacgcgg cgatcgtatt ggctgggtta agggcattga tgataactgg cacctgacgc
     9181 tgtttatcga aaatggtcgc atccttgatt atccggggcg tccgctgaaa actggcctgc
     9241 tggagatcgc gaagatccac aaaggtgatt tccgcattac ggcgaaccag aatctgatca
     9301 tcgccggtgt gccggaaagc gagaaagcga agatcgagaa aattgccaaa gagagcgggt
     9361 taatgaatgc cgtcacgccg cagcgtgaaa actcgatggc ctgcgtgtca ttcccgactt
     9421 gcccgctggc gatggcggaa gcggagcgtt tcctgccgtc ttttatcgac aacatcgata
     9481 atttaatggt gaaacatggt gtcagcgatg agcatatcgt gatgcgtgta acaggctgcc
     9541 cgaacggttg tggtcgcgcg atgctggcgg aagtgggcct ggtgggtaaa gcaccgggtc
     9601 gctacaacct gcatcttggc ggcaaccgca ttgggacacg tatcccacgg atgtataaag
     9661 aaaacatcac cgagccggaa atcctggcgt cgcttgatga actgataggg cgctgggcga
     9721 aagagcgcga agcgggtgaa ggcttcggcg actttacggt gcgtgcgggc atcattcgcc
     9781 cggtgctcga tccggcgcgc gatttgtggg attaaccatc agcccggtct tgtaggcctg
     9841 ataagacgcg tgagcgtcgc atcaggcaag gcaaacagtg aggaatctat gtccaaactc
     9901 gatctaaacg ccctgaacga actgccgaag gtagatcgca ttctggcgct ggcagaaact
     9961 aacgccgaac tggaaaaact ggacgctgaa ggccgcgttg cctgggcgct ggataatctg
    10021 cccggtgaat atgtactttc ttccagcttt ggcattcagg cggcggtgag cctgcatctg
    10081 gtgaatcaga ttcacccgga tattccggtg atcctcaccg acaccggcta tctgttcccg
    10141 gaaacctacc gctttattga cgagttaacg gacaaactca agctcaacct gaaagtgtac
    10201 cgcgctaccg aaagcgccgc ctggcaggaa gcacgctacg gcaaactgtg ggaacagggc
    10261 gttgaaggca ttgaaaagta caatgacatc aacaaagtcg aaccgatgaa ccgggcgctg
    10321 aaagaactga atgcgcaaac gtggtttgct ggcctgcggc gtgaacaatc cggcagccgt
    10381 gccaatttac cggtgctggc aattcagcgt ggcgtattta aagtgctgcc gattatcgac
    10441 tgggataacc gaactattta tcagtacctg caaaaacatg gcctgaaata tcacccatta
    10501 tgggacgaag gatatttatc ggtgggcgat acccatacaa cccgtaaatg ggaacccggc
    10561 atggcggaag aagaaacgcg tttctttggc ttaaaaaggg aatgtggatt acacgaaggg
    10621 taaatattgc cggataaatt aaacgcctct gtcagaaatg atgggggcgt ttttattttg
    10681 cgagcagcat tccggcattt aaaggtgaac atcgcaggga aaattaacag gtatagaatg
    10741 gcgttgggtg cttgaggctg tctggcgtta agcattcgcg aggttccaga tggacaaaag
    10801 ccccaggcga tatttctatc aacctgaggc tcgcgttcga acgtagcaat tcggatgtta
    10861 gtctcttctt tgcagggagg caagacatgc tgacaaaata tgcccttgtg gcaatcatcg
    10921 tactgtgttg tacagtactg ggattcacgt tgatggtagg tgactcgtta tgtgagttga
    10981 gtatcagaga acgtggtatg gagtttaagg cagttctcgc ttacgaatcg aagaagtagc
    11041 caccacgcgg gggtaaaacc ccgcgtactg aattgtgatg gtttggtctc ctgcaccctt
    11101 tttttaaatt tatatttttt atgtgaatat atagtctcta tttattatta tgaatttcaa
    11161 atgaaaatca ttaatttatt cataatgtaa atgttttatt ggctgatata tatcatagat
    11221 agtttttaaa atatgcgtaa atatccttta agtttactga aggataaaaa tattgtgact
    11281 ttctttgatt tctggggaaa aaaccgacgt ggcgagaaag agggtatcga cgactatcac
    11341 cttctttgct ggcatttgct ggatgtggcc gcaatgggct atttaatggt taaaagaaat
    11401 tgcttcgggc tggctgatta ttttcgtcaa ttagggattt ctgacaagga acaggcagct
    11461 cgatttttcg cctggttgct gtgctggcac gatattggaa aatttgcccg ctcttttcaa
    11521 caactttacc tgccccctga attcaagatt caggaaggtg ccagaaaaaa ttacgaaaaa
    11581 atctctcatt caacgctggg ctactggttg tggaatcatt atttaagtga atgtcaggag
    11641 ttacttcctt catcttcgct ctctccgcgc aaacttaggc gagtgataga aatgtggatg
    11701 cccgtgacga cagggcatca tggtcggccc cctgatcgta tggatgaatt ggataatttt
    11761 ctgcctgaag acaaagctgc agcacgagat tttctccttg aaatcaagcc actgtttccg
    11821 cgaatagaga tccccacatt ctgggatgat gacgagggcg ttgaacttat aaaacaactt
    11881 tcctggtata tctctgcaac cgtcgtactt gcagactgga cagggtcgtc aacgcgattt
    11941 tttccacgcg tcgcacaggc aatggatatt gaagattact ggcaaaaggc tttagttcag
    12001 gctcaaaacg cattaaccgt ctttccgcca caagcagaaa ccgcgccttt caccggaatt
    12061 aatacgctgt ttccttttat tgagaatacg acaccgttac agcaaaaggt gctggatctg
    12121 gatatcaatc agccaggacc acagttgttt attctggaag atgtgaccgg cgcaggtaaa
    12181 acagaagcgg cgcttatcct ggcgcacagg ttgatggctg cgggtaacgc tcaggggctg
    12241 ttttttggcc tgccaacaat ggcaacggcc aatgccatgt acgatcgact ggtcaaaacc
    12301 tggcttgctt tctattcgcc agaggtgttc cccgctccag cggagataaa ccggcgcaat
    12361 acggtgcggt tttcccggaa gtaaaccgga cgcactggat gcgatgatgg gtatcactta
    12421 gaattccccg cccctgcggt agaactccca gctcccattt tcaaacccat caagacgcct
    12481 tcgccagctc cttcaccaac ggtagcatta tccgcataac gtcacggcag cgacgttcta
    12541 ttcttccagg aagtgcctta tcaatatgtt gttgattatc cagtcttacg tcatgccagc
    12601 tatttcccgc cgggaaggca ggtgtttttg cgcgttgctg ataaccatcc ttatttccaa
    12661 gattccagtt agtcgcttcc accgaaagta cagcaatgcc cgctttgtcg aatatttctg
    12721 cgtcattaca acccccagtg cctttcggat aatttttatt caaacccgga ttggtcgttg
    12781 cggctattcc gtgactgcgt gcaattgcca gcgccctgtc gcgcgttaat ttccttactg
    12841 cttcaggggt ttttacaccg ctgttgaaat acaatttatc gccaacaatt aagttatcga
    12901 gattaatcac cagcagcgta tttttctttt cggtgtcact catccgcttg agtaaattct
    12961 cagcgcctaa ttttccttcc tcttcgccgc tggtcgccac aaatcgaata ccatactcgg
    13021 taggcgtatt tttcaggcgt tctgccagtt ccagcatgac acctaaacct gcggcgttat
    13081 catctattcc ttgtaacgtc agtccgccga gattggcatc tgcgtcagca tcgctcagcg
    13141 gggcgtaagt atccagatgc gccataatga tgatctgctg cggcgctttg ccttcatgag
    13201 cggcaatcac cgtacttcct gtcacgttat gccagttctt gcgattatcg cgggcggtat
    13261 aaatataccg actattaaat gtccgaatat cactgcgata acccatttgc tgaaactgtt
    13321 ggcgaatata atcggcagat aacatttctg ctggagttcc ggtcatgcgt cccggaaaga
    13381 aagtagcaat atgtcgtgcc tgggtattag caaaatcgcc aggtttaggt gacgaggcgt
    13441 gtacggggag aataaagcat acgccgagcg ccagggcagc ggtacggtgg cgcaatgcgg
    13501 aaaacatagt gagtccttaa ataccatgca aattttttta ccgccatagt atgaaactgc
    13561 cgctgcgcta aaacaatttc aaatcttcct aaacgcccga aatccggtgc cttaagcact
    13621 ttttgatatt agctttgcca aatcgttatt ccgttaagga actactcatt ctaattggta
    13681 atttcattcg ttctcttacg ctccctatag tcgaaacatc tgatggcaag aaaatagcgg
    13741 tattgcaaag gaacggttat ggctcaaaaa cgacttactc acctgcggca actggaggcg
    13801 gaaagcatcc acattattcg cgaggtggcg gcagaattct caaatccggt gatgctctac
    13861 tctatcggta aagattccag cgtcatgctg catctggcgc gcaaggcgtt ttatccaggt
    13921 acgctgcctt tcccgttgct gcatgtcgat accggctgga aattccgcga gatgtatgag
    13981 ttccgcgatc gtacggcgaa agcctacggc tgcgagctgc tggtgcataa aaacccggaa
    14041 ggcgtggcga tggggattaa tccattcgtg cacggcagcg cgaaacatac cgatattatg
    14101 aaaactgaag gcctgaaaca ggcgctgaac aaatacggtt ttgatgccgc cttcggtggt
    14161 gcgcgccgtg acgaagagaa atcccgcgct aaagagcgaa tctactcttt ccgtgaccgc
    14221 ttccatcgct gggatccgaa aaatcagcgc ccggagctgt ggcacaacta caacgggcaa
    14281 attaacaaag gcgaaagcat ccgcgtcttc ccgctctcta actggaccga gcaggatatc
    14341 tggcaataca tctggctgga aaatatcgac attgtgccgc tgtatctcgc tgcggaacgt
    14401 ccggttctgg aacgcgacgg tatgttgatg atgattgatg acaaccgtat cgacctgcaa
    14461 ccgggcgaag tgatcaaaaa acggatggtg cgtttccgta cgctgggctg ctggccgctg
    14521 accggtgcgg tggagtcaaa tgcacaaaca ctgccggaaa tcatcgaaga gatgctggtt
    14581 tccaccacca gtgaacgtca gggtcgcgtg attgaccgcg accaggcggg atccatggag
    14641 ctgaaaaaac gtcaggggta tttttaagat gaacaccgta cttgcacaac aaatcgccaa
    14701 tgaaggcggc gtcgaagcct ggatgattgc gcaacaacat aaaagcctgc tgcgttttct
    14761 gacctgtggt agcgtcgatg acggcaaaag taccctgatt ggtcgtctgc tgcacgatac
    14821 ccgccaaatc tacgaagatc agctctcatc gctgcataac gacagtaagc gtcacggaac
    14881 ccagggcgaa aagctggatc tggctctgct ggtggacggc ctgcaagctg agcgcgaaca
    14941 gggcatcact attgatgtgg cctaccgcta tttctctacc gagaagcgta aatttattat
    15001 cgccgacacc ccagggcacg agcagtacac tcgcaatatg gcgactggcg catcgacatg
    15061 tgaactggcg atcttactga ttgatgcccg taaaggcgtg ctcgatcaaa cccgtcgtca
    15121 cagttttatc tccacactgt tggggatcaa acatctggtc gtggcgatca acaaaatgga
    15181 tctggtggat tacagtgaag agacgttcac ccgtattcgt gaagattatc tgacctttgc
    15241 cgggcagctg ccgggtaatc tggatatccg ctttgtgccg ctctccgcac tggaaggtga
    15301 caacgtggct tcgcaaagtg aaagtatgcc gtggtacagc ggtctgacac tgctcgaagt
    15361 gctggaaacc gtggagatcc agcgagtggt ggatgcccag ccaatgcgct tcccggtgca
    15421 gtacgttaac cgcccaaatc tcgattttcg tggctacgcc ggaatgctgg catccggtcg
    15481 cgtggaagtc gggcaacgtg taaaagtgct gccctctggt gtggaatcaa acgtcgcgcg
    15541 gatcgtgact tttgatggtg atcgcgaaga agtctttgcc ggagaagcga tcaccctggt
    15601 gctgacggat gagatcgata tcagccgtgg tgatctgctg ctggcggcag acgaagcgtt
    15661 accggcggtg cagagtgcgt cggtggatgt ggtatggatg gcggaacagc cgctttcccc
    15721 gggccagagt tatgacatca aaattgccgg taagaagacg cgtgctcgtg ttgatggcat
    15781 tcgttatcag gttgatatta ataaccttac ccaacgcgaa gttgaaaacc tgccgctgaa
    15841 cggcatcggc ctggtggatc tcacttttga cgagccactg gtgttagatc gctatcagca
    15901 aaatccggtt accggtgggc tgatttttat cgatcgcctg agcaatgtga ccgtgggtgc
    15961 cggtatggtg cacgagccag ttagccaggc aacagctgcg tcatctgaat tcagtgcatt
    16021 cgaactggaa ttgaatgccc tggttcgtcg ccactttccg cactggggcg cgcgcgattt
    16081 gctgggggat aaataatggc gctgcatgac gaaaacgtcg tctggcatag tcatccggtc
    16141 actgtgcaac aacgcgaact acaccacggt catcgtggtg tagtgctgtg gtttaccggc
    16201 ctctccgggt ccggtaaatc aacggtcgcc ggggcgctgg aggaggcgtt acataaactc
    16261 agcgtcagta cgtatctgct ggatggcgac aatgttcgcc acggattatg cagcgatctc
    16321 ggttttagcg atgccgatcg taaagagaat atccgtcgcg tcggtgaagt ggcgaatttg
    16381 atggttgaag ccggactggt ggtgctgacc gcatttatct cgccacaccg cgccgaacgc
    16441 cagatggttc gcgaacgcgt aggagaaggg cgctttattg aagtgtttgt cgatacgccg
    16501 ctggcgattt gcgaagcccg cgatccaaaa ggcttatata agaaagcgcg tgccggtgaa
    16561 ctgcgcaact ttacgggaat agattccgtt tacgaagcgc ctgaatcggc agaaattcat
    16621 ctcaatggtg aacaattagt aacaaatttg gtacagcaat tattagatct gttgagacag
    16681 aacgatatta tcagatcctg agacaccacc gggtttccat gcccggttcg tcaaagtaac
    16741 aggatcaaat atgcgtaata gccataacat tacactaaca aataacgaca gccttactga
    16801 ggatgaagaa actacatggt cactgcctgg tgccgtggtc ggttttatct cctggttatt
    16861 tgcgctggcg atgccgatgt tgatttatgg ctctaacacg ctgttcttct ttctctacac
    16921 ctggcctttc tttctggcac tgatgcctgt cgcggtagtg gtggggattg cgctgcattc
    16981 attgatggac ggaaagctac gctacagtat tgttttcacc ctggtgacag taggcattat
    17041 gtttggcgca ctgtttatgt ggctactggg ctaatttgta ctttcttgtc tcctctgttc
    17101 ataattcaaa ccgtaactaa taatgagatt atgttctgca cgccctgggt atacgtaaca
    17161 atgggcaaat gtggtacatt tgcccgcgtt gtcgcggtat ccccaacaga ggatgtagag
    17221 tcgtcttcgg atgcatggga tgatgatgca gtttttcagg gggcaggatg ggtaaactaa
    17281 cgctgctgtt gctggctatt ctggtctggc tacagtattc gctgtggttc ggtaagaacg
    17341 gtatacatga ctatacccgc gtcaatgatg atgtggcggc actgcaagct acaaacgcga
    17401 aacttaaagc gcgaaacgat caactttttg ccgaaattga cgatctcaat ggcggccagg
    17461 aggcgctcga agagcgtgcg cgtaatgaac tcagcatgac caggccgggc gaaacttttt
    17521 atcgtctggt gcctgacgcg tcgaagcgcg cacagtctgc ggggcaaaac aatcgataaa
    17581 tcagcccggg aattaacatg gcaaccactc atttggatgt ttgcgccgtg gttccggcgg
    17641 ccggatttgg ccgtcgaatg caaacggaat gtcctaagca atatctctca atcggtaatc
    17701 aaaccattct tgaacactcg gtgcatgcgc tgctggcgca tccccgggtg acacgtgtcg
    17761 tcattgccat aagtcctggc gatagccgtt ttgcacaact tcctctggcg aatcatccgc
    17821 aaatcaccgt tgtagatggc ggtgatgagc gtgccgattc cgtgctggca ggtctgaaag
    17881 ccgctggcga tgtgcagtgg gtattggtgc atgacgccgc tcgtccttgt ttgcatcagg
    17941 atgaccttgc gcgattgttg gcgttgagcg aaaccagccg cacgggaggg atcctcgccg
    18001 caccagtgcg cgatactatg aaacgtgccg aaccgggcaa aaatgccatt gctcataccg
    18061 ttgatcgcaa cggcttatgg cacgcgctga cgccgcaatt tttccctcgt gagctgttac
    18121 atgactgtct gacgcgcgct ctaaatgaag gcgctaccat taccgacgaa gcctcggcgc
    18181 tggaatattg cggatttcat ccgcagttgg tcgaaggccg cgcggataac attaaagtca
    18241 cgcgcccgga agatttggca ctggccgagt tttacctcac ccgaaccatc catcaggaga
    18301 atacataatg cgaattggac acggttttga cgtacacgcc tttggcggtg aaggcccaat
    18361 tatcattggt ggcgtacgca ttccttacga aaaaggattg ctggcgcatt ctgatggcga
    18421 cgtggcgctt catgcgttga ccgatgcatt gcttggcgcg gcggcgctgg gggatatcgg
    18481 caaactgttc ccggataccg atccggcatt taaaggtgcc gacagccgcg agctgctacg
    18541 cgaagcctgg cgtcgtattc aggcgaaggg ttataccctt ggcaacgtcg atgtcactat
    18601 catcgctcag gcaccgaaga tgttgccgca cattccacaa atgcgcgtgt ttattgccga
    18661 agatctcggc tgccatatgg atgatgttaa cgtgaaagcc actactacgg aaaaacttgg
    18721 atttaccgga cgtggggaag ggattgcctg tgaagcggtg gcgctactca ttaaggcaac
    18781 aaaatgattg agtttgataa tctcacttac ctccacggta aaccgcaagg caccgggctg
    18841 ctgaaagcca atccggaaga ctttgtggtg gtggaagatt tgggctttga gcctgatggt
    18901 gaaggtgagc atattctggt tagaatcctc aaaaacggct gcaatacccg ttttgtggcg
    18961 gatgcactgg cgaaattcct gaaaattcat tcccgtgaag tcagcttcgc tgggcaaaaa
    19021 gacaaacatg ctgtaacgga acagtggtta tgtgctcgcg tgccgggcaa ggaaatgccc
    19081 gatctgagcg cctttcaact ggaaggctgc caggtgctgg agtatgcgcg gcacaagcgc
    19141 aagctgcgtt taggggcgct gaaaggtaac gcctttaccc tggttctgcg cgaagtgagc
    19201 aatcgcgatg acgttgaaca acgtctgatc gatatttgcg taaaaggtgt accgaactac
    19261 ttcggtgccc aacgttttgg gattggcggt agcaacttgc agggcgcgct gcgctgggcg
    19321 caaaccaata ctccggtgcg cgatcgcaat aaacggagtt tttggttgtc ggcagcccgc
    19381 agtgcgttgt ttaatcagat tgttgctgag cgcctcaaaa aagcagacgt taatcaagtt
    19441 gttgacggcg atgcgctaca attagccgga cgtggtagct ggtttgtagc aaccaccgaa
    19501 gaactggcgg aattacagcg tcgcgtcaac gataaagagc tgatgataac cgccgcattg
    19561 ccgggcagtg gcgaatgggg aactcagcgt gaagcgctgg cattcgaaca agcagctgtc
    19621 gccgcagaaa ctgaattaca agctttactg gtgcgcgaaa aagttgaagc cgcgcgcaga
    19681 gcgatgctgc tgtatccgca acaattaagc tggaactggt gggatgacgt caccgtagag
    19741 atccgtttct ggcttccggc gggtagtttt gcaaccagcg ttgtcaggga acttatcaac
    19801 acaacaggtg attatgcgca tattgctgag taattatgac ggggtacatg cacccggtat
    19861 acaaacgctg gcgaaagcct tgcgtgagtt tgctgacgtt caggtggtcg cccccgatcg
    19921 taaccgcagc ggcgcttcaa attctctgac actggaatcc tccctgcgca cgtttacctt
    19981 tgaaaatggt gatattgctg tgcaaatggg aaccccgacc gattgtgtct atcttggcgt
    20041 gaatgctctg atgcgtccgc gcccggacat tgttgtgtcc ggaattaacg ccgggccgaa
    20101 tctgggggat gatgttattt attccggtac ggtagccgcc gcgatggaag gccgtcattt
    20161 aggttttccg gcgctggccg tctcgcttga cgggcataaa cattacgaca ctgccgcggc
    20221 ggtaacctgt tcaattttgc gcgcactgtg taaagagccg ctgcgcaccg gacgtattct
    20281 taatattaac gttcaggatt tacccttgga tcaaatcaaa ggtattcgcg tgacgcgctg
    20341 cggtacacga catccggcag atcaggtgat cccgcagcaa gatccgcgcg gcaatacgct
    20401 gtactggatt ggcccgccgg gcggtaaatg tgatgccggt ccggggaccg attttgctgc
    20461 ggtagatgag ggctatgtct ccatcacgcc gctgcatgtg gatttaactg cgcatagcgc
    20521 gcaagatgtg gtttcagact ggttaaacag cgtgggagtt ggcacgcaat ggtaagcaga
    20581 cgcgtacaag cacttctgga tcaattacgt gcgcaaggta ttcaggatga gcaggtgctg
    20641 aatgcacttg ccgccgtgcc gcgtgaaaaa ttcgttgatg aagcgtttga acaaaaagcc
    20701 tgggacaata tcgccttgcc gataggtcag gggcagacaa tttcgcagcc gtatatggtg
    20761 gcgcgaatga ccgaattact cgagctgacg ccgcagtcgc gggtgctgga aattggcact
    20821 ggttcgggat atcaaacggc aatcctggcg catcttgtcc agcatgtttg ctcggttgaa
    20881 cggattaaag gcttgcagtg gcaggcacgt cgccgcctga aaaatcttga tttacataat
    20941 gtttcaaccc gtcatggtga tggatggcaa ggttggcagg cacgtgcgcc gtttgacgct
    21001 atcattgtta cggcggcacc gccggaaatt ccaactgcgc taatgacgca gctggacgaa
    21061 ggcgggattc tcgtcttacc cgtaggggag gagcaccagt atttgaaacg ggtgcgtcgt
    21121 cggggaggcg aatttattat cgataccgtg gaggccgtgc gctttgtccc tttagtgaag
    21181 ggtgagctgg cttaaaacgt gaggaaatac ctggattttt cctggttatt ttgccgtagg
    21241 tcagcgtatc gtgaacatct tttccagtgt tcagtagggt gccttgcacg gtaattatgt
    21301 cactggttat taaccaattt ttcctggggg ataaatgagc gcgggaagcc caaaattcac
    21361 cgttcgccgc attgcggctt tgtcactggt ttcgctatgg ctggcaggct gttctgacac
    21421 ttcaaatcca ccggcaccgg tcagctccgt taatggcaat gcgcctgcaa atactaattc
    21481 tggtatgttg attacgccgc cgccgaaaat ggggacgacg tctacagcgc agcaaccgca
    21541 aattcagccg gtacagcagc cacaaattca ggctactcaa caaccgcaaa tccagccagt
    21601 gcagccagta gctcagcagc cggtacagat ggaaaacgga cgcatcgtct ataaccgtca
    21661 gtatgggaac attccgaaag gcagttatag cggcagtacc tataccgtga aaaaaggcga
    21721 cacacttttc tatatcgcct ggattactgg caacgatttc cgtgaccttg ctcagcgcaa
    21781 caatattcag gcaccatacg cgctgaacgt tggtcagacc ttgcaggtgg gtaatgcttc
    21841 cggtacgcca atcactggcg gaaatgccat tacccaggcc gacgcagcag agcaaggagt
    21901 tgtgatcaag cctgcacaaa attccaccgt tgctgttgcg tcgcaaccga caattacgta
    21961 ttctgagtct tcgggtgaac agagtgctaa caaaatgttg ccgaacaaca agccaactgc
    22021 gaccacggtc acagcgcctg taacggtacc aacagcaagc acaaccgagc cgactgtcag
    22081 cagtacatca accagtacgc ctatctccac ctggcgctgg ccgactgagg gcaaagtgat
    22141 cgaaaccttt ggcgcttctg aggggggcaa caaggggatt gatatcgcag gcagcaaagg
    22201 acaggcaatt atcgcgaccg cagatggccg cgttgtttat gctggtaacg cgctgcgcgg
    22261 ctacggtaat ctgattatca tcaaacataa tgatgattac ctgagtgcct acgcccataa
    22321 cgacacaatg ctggtccggg aacaacaaga agtgaaggcg gggcaaaaaa tagcaaccat
    22381 gggtagcacc ggaaccagtt caacacgctt gcattttgaa attcgttaca aggggaaatc
    22441 cgtaaacccg ctgcgttatt tgccgcagcg ataaatcggc ggaaccaggc ttttgcttga
    22501 atgttccgtc aagggatcac gggtaggagc caccttatga gtcagaatac gctgaaagtt
    22561 catgatttaa atgaagatgc ggaatttgat gagaacggag ttgaggtttt tgacgaaaag
    22621 gccttagtag aagaggaacc cagtgataac gatttggccg aagaggaact gttatcgcag
    22681 ggagccacac agcgtgtgtt ggacgcgact cagctttacc ttggtgagat tggttattca
    22741 ccactgttaa cggccgaaga agaagtttat tttgcgcgtc gcgcactgcg tggagatgtc
    22801 gcctctcgcc gccggatgat cgagagtaac ttgcgtctgg tggtaaaaat tgcccgccgt
    22861 tatggcaatc gtggtctggc gttgctggac cttatcgaag agggcaactt ggggctgatc
    22921 cgcgcggtag agaagtttga cccggaacgt ggtttccgct tctcaacata cgcaacctgg
    22981 tggattcgcc agacgattga acgggcgatt atgaaccaaa cccgtactat tcgtttgccg
    23041 attcacatcg taaaggagct gaacgtttac ctgcgaaccg cacgtgagtt gtcccataag
    23101 ctggaccatg aaccaagtgc ggaagagatc gcagagcaac tggataagcc agttgatgac
    23161 gtcagccgta tgcttcgtct taacgagcgc attacctcgg tagacacccc gctgggtggt
    23221 gattccgaaa aagcgttgct ggacatcctg gccgatgaaa aagagaacgg tccggaagat
    23281 accacgcaag atgacgatat gaagcagagc atcgtcaaat ggctgttcga gctgaacgcc
    23341 aaacagcgtg aagtactggc acgtcgattc ggtttgctgg ggtatgaagc ggcaacactg
    23401 gaagatgtag gtcgtgaaat tggcctcacc cgtgaacgtg ttcgccagat tcaggttgaa
    23461 ggcctgcgcc gtttgcgcga aatcctgcaa acgcaggggc tgaatatcga agcgctgttc
    23521 cgcaagtaag taagcatctg tcagaaaggc cagtctcaag cgaggctggc ctttttcttt
    23581 tgtttgggaa gcttgtgttt ttgtcgcctg gataagacac gtcagcgtcc tacccggcaa
    23641 tcagcattct atgtgctcat catcttgcgc accaactgca taaacagctc ctgctcttct
    23701 ccactcaaac gccccaaaaa ttcctcatcc acgctgtcac caataggtat cgccgcagca
    23761 agtagctttt ctccttcggc agtcagccag acaaagcgtc gccgcttgtc agcgacgtca
    23821 tgttcacgtc tgactaagcc acgattttcc attcttgcca acatttctgc tagcgttgct
    23881 ttggtgctga ctgctgcttc tatcagtgcc acctgttcaa taccaggttt atcagcaatg
    23941 gcgcgcatca ccgcgtactg tggtttggtc aggtcaggta actcgtgctg ccagcgagcc
    24001 gtgtgctgct gaaaaagctg tcgtaactga tggaacgctt tatttcgtaa cgccatgtaa
    24061 actccgggct aattctctgt cgctatcata gcggtttttt agtgacgaag aagatgttgt
    24121 tacttttcaa tccttgccgc tgtggaaatg aaggagtatg ttaataataa cgttcgtata
    24181 cgaataatta agagaataag caatgaaact gatcgtcggg atgacagggg ctaccggtgc
    24241 gcctcttggt gtggcattac tgcaagcgct gcgtgagatg ccgaatgtcg agactcatct
    24301 ggtgatgtcg aagtgggcga aaaccaccat tgaactggaa acgccttaca gcgctcgcga
    24361 tgttgctgcc ctcgcagact tcagccataa cccggcggat caggcggcga tcatctcatc
    24421 cggttctttt cgtaccgacg gcatgatcgt tattccgtgc agtatgaaaa cgctcgccgg
    24481 tatccgcgct ggttacgccg atggcctggt agggcgcgcg gcggacgtcg tgctcaaaga
    24541 aggccgcaaa ctggtgctgg tgccgcgtga aatgccgctt agcaccatcc atctcgaaaa
    24601 tatgctcgca ctttcacgca tgggcgtggc gatggtgccg ccgatgcctg ccttttataa
    24661 ccatcccgaa acggtagatg acattgtcca ccatgtggta gcccgcgtgc tggatcaatt
    24721 tggcctcgaa catccccacg ccaggcgctg gcaaggattg ccgcaggccc ggaatttttc
    24781 tcaggagaat gaataatggc atttgatgat ttacgcagct ttttacaggc gcttgatgac
    24841 cacggccagt tactgaaaat cagcgaagaa gtgaacgctg agccggatct ggcagcagca
    24901 gctaacgcca ccgggcgtat cggcgacggc gcgcccgcgc tgtggtttga taatattcgc
    24961 ggctttaccg atgcccgcgt ggcgatgaat accatcggtt cctggcagaa ccacgcgatt
    25021 tcccttggcc tgccaccaaa caccccggtc aaaaagcaga ttgatgagtt tatccgccgc
    25081 tgggataact tcccgattgc cccggagcgc cgcgccaatc cagcctgggc gcagaacacc
    25141 gtcgatggcg aagagatcaa cctgttcgat attctgccgc tgtttcgcct gaatgatggc
    25201 gatggcggtt tctatctcga caaagcgtgc gtggtttccc gcgatccgct cgacccggat
    25261 aacttcggca agcagaacgt cggcatctac cgcatggaag tgaagggcaa gcgtaagctc
    25321 ggcctgcaac cggtgccgat gcacgatatc gccctgcatc tgcataaagc agaagagcgc
    25381 ggtgaagatc tgccgattgc gatcacgctc ggtaacgatc cgatcatcac gctgatgggg
    25441 gccacgccgc tgaaatacga tcagtctgag tatgaaatgg caggcgcgct gcgtgaaagc
    25501 ccgtacccga tcgccaccgc tccgctgacc ggttttgatg tgccgtgggg ttcagaagtg
    25561 atcctcgaag gggttatcga aagccgtaaa cgcgaaatcg aagggccgtt cggtgagttt
    25621 accgggcact actccggtgg gcgcaacatg accgtggtgc gcatcgataa agtctcttat
    25681 cgcagcaaac cgattttcga atcactctat ctcggtatgc cgtggaccga aatcgactac
    25741 ctatgactag gtaatttatt caagataacc gggaagagat taaaaaagac gggaatagat
    25801 tggaatcaac aagttaggaa ctcaaggttt tcaagataaa gcgagtccaa aaatgttaaa
    25861 cggttcgata tcaataaaac attcaagata actcacaggt tcttacaata gcctttaaag
    25921 accgttcaaa aacgattcag gtgtttatta tagcgctgat gggtgcaagc gccaaaacca
    25981 atgtaatttt agcacagcta ggcacttgca ctgggttatg cgctggctgc tttctgaggt
    26041 agatttaagt gatcgccctt agcttcggac aacgcgtaaa tctctctaac cttttcgtct
    26101 ggcaaattac caaggcttaa cgctatggct ctacgaactc caactagagc gtgcaactct
    26161 tggatgtgct catccagaag aatgagtaat tgctccgctc cttttctccc aagaacatca
    26221 gctacttgtt gtttttcttg tggtttaaga aatgacaaga acattagaat ttgagcatca
    26281 acattatccg cattctcttt tttgggttct ttggaatctt ttagttcaac atcaccctct
    26341 cccgtcatga gccattccaa ggtaacattt tcgctttctg ctatcttttt taactgatac
    26401 gggcgtggta tcggtgcgta atcccgcctg ttgtaataat tttttaaagt gccttcattg
    26461 atcccccatg cccgagcagc cgcatttctt gaagggtatc gttctactaa cctaaagatt
    26521 ctttcgataa tcggttcttt tccatcggca ggaaaagaac cttggggtgc ttttctcatt
    26581 cttggttcct ttctatctaa ttcattgata aataataaaa ttaaaaaata aaccataaaa
    26641 acaccaaaga accctttgca gggaatcttt ggtatcttaa tctttatcca tacggatatc
    26701 cgaccggata tccgcgcggg taattttgta ggttaaagga aagatgagac aaagagaaga
    26761 tagtgaccgc gattggcctt cgctgaagat tcagctggcg ctcaaagata acgggtggtc
    26821 ttccttccgg gagctttcac tccatgctgg ttatgctcag gattcactga aagcggtagt
    26881 tcgcacacca aacaaaaaat acgaacgcgt aatttctgac gctataggtg taccgccaga
    26941 aatcatctgg cccagccggt atcgtaacga tggatttatc aggaaggcgt cgtgatatgc
    27001 agtcgtacgt taccgtaaat gacctgctcg gcgtaccagg aatgccagcc accacaaaag
    27061 gcatccgaca ggcgttacag cgtttttctc gtgatcttgg tgacgttagt cgccgtcggg
    27121 aaggcacaaa agccattgag taccacattg attgtctgcc tgaaataaca cgaaaggcac
    27181 tgcgtgaacg ctatgtggaa cagctggtgg ctacagagaa caatgtttct gaagtgaaag
    27241 ctgttaccag aaaaacacgc aatcctgacg ctgtccaggc aatcgaagca tatcgcggtt
    27301 ctccacaact gatggaagaa cgcctgaatg cgctgaccga aaaccagcgc tgggtatctg
    27361 aagcaagagc tgcgctggtg gtggaagtgc tgaagctgga aagcgccggt aaccccgggc
    27421 gactgaaagc cattaacttt cttgttgaaa aagcccgtaa aggtgagctg ccggagcgcc
    27481 tgcaacaggc cgcagttaac gccaatgcaa aacgtggcgc taatcgtact atcagccgcg
    27541 atccacttta ccagtgggtt ctgaaataca accagtcgca gaacgccgcc gaacggctgc
    27601 tgttactggc accgggaaaa cgcgacgaaa taaaaccgga agaaatcagc tggctgcctg
    27661 aatttctggc gcagtatcgc caggtgaacg gcagacccat gtcagaagcc tacgaggatt
    27721 ttgtcgcaga atggcagcgt cgccatgcgg acgaaccgta catgctggag gtgatgcctt
    27781 cctatgatgt tgtccggtat gccatgaaaa aactgccgga agtggtgaaa caaaaagggc
    27841 gcgttaccgg cagtgaatac cgtcagcttg aaggattcac ccgccgcgac tggacagcta
    27901 tgccggtgaa ttatgtctgg attggtgacg gtcacggtat gaagctgaaa tgtgcgcatc
    27961 cgatccacgg gcgtccattc tcaccggaag tcacgtttgt gatcgatggt ggaacacggt
    28021 ttgttgttgg ctggagtctt gaccttgccg aaaacgtgtt cgccgttgcc ggtgcgatac
    28081 agcacggcat tcgcaaccac ggaaaaccgt tcctgtatta ctcggataac ggttccggtg
    28141 aaacggctga catgctggac aaagaggtcg tgggtattct gccacgcctg gggattaaac
    28201 accctaccgg tattgccggt aatccacagg gacgcggcat tattgaacgc ctgaaccgga
    28261 cactgccgat gcgtatagcc aggaggtatc gcacctattt tggcaagggt gctgaccggg
    28321 aatcgttgcg tgtacttaac cgtgatctgc gttcagcgtt taacgccctg caacaggaca
    28381 agcctctgaa cgaccgccag aaagcggcaa tgcgcgagct gccatcgtgg gcggagctta
    28441 ttgaggctat tcgtgaaggc gtggaatggt acaacaaccg cccgcattct gagctgccga
    28501 tgaaaccgaa cggacggcat tacagcccga cagagttcag aaaaaaacgc caggcagaag
    28561 aagacaccga aattgagtgg ctgagtgatc ttgagctgcg cgacatgttc cgcccaatgg
    28621 tggaacgacc ggtaagacgc tgcgaaattc agtggctgaa caatatttac tacgcgccgg
    28681 agttgcgtga cgaacacggt cgcaaagtgc ttatcagtta tgacattcat gacgccgagc
    28741 gaattacggt acgtcgcaag gacggcagct ttatctgtga ggcgatatgg aacggcaata
    28801 aacgcgcagc c
//
LOCUS       DAJTRC010000050        28654 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.050, whole genome shotgun sequence.
ACCESSION   DAJTRC010000050 DAJTRC010000000
VERSION     DAJTRC010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28654)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28654)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28654
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.050"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            1..638
                     /locus_tag="OO357_002286"
                     /pseudo
     CDS             1..638
                     /locus_tag="OO357_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000163825.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
     gene            635..1021
                     /locus_tag="OO357_002287"
     CDS             635..1021
                     /locus_tag="OO357_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887214.1"
                     /translation="MKFFCIIVIFLSINYIAIVNVFKSSAVAQSTKYYEENDIHDGSN
                     DIFFMKKYGPPAHEFVRPRYKFVGEYYIGLNQTYRITDPEFQDVPIKEMFWNLQGDLN
                     LTCWFHYKDGQWRVITYIFWPPGARF"
     gene            1046..3259
                     /gene="tssI"
                     /locus_tag="OO357_002288"
     CDS             1046..3259
                     /gene="tssI"
                     /locus_tag="OO357_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein VgrG"
                     /protein_id="HCR5887215.1"
                     /translation="MSFVSTGNKPVGNGGPVITTPPIAGESGGMSTGSAVTDVSGAAE
                     EMAEQAAADLFGALPEPSGLVKAAVAAAQAAAAAAGISDMAGAVQDAAASLAAGAPGA
                     HNVTVSGSAVPPQMLLFAGMNGSEGLGNLFSYTVQLKTLDALNLGYVSPAANLPLQLM
                     VGKDLCVSIELDGGGKRYISGLVTAARVVGHESRSVTYELRIEPWLKLLTHTSDYKAF
                     QNKTVVDILDEVLDEYSFPVEKRLVENYPPRAWQVQYGETDFDFIQRLMQEWGIYWWF
                     EHSENSHTLVLVDAINGHKACPDSPLVEWHQEGLKLDKEFIHTITASERLRTGKWVMD
                     DFDFMKPRSLLKSTVASPRDTGHAEYEHYEWPGDYFTTGEGEMLTRIRMEAQRSPGSR
                     AHGAGHIRTLMTGYTFTLMNHPTAEINQEYLLVQTTLFLRDNAQHSGQDQHFTYVTTF
                     ELHPTSEVYRPQRTLSKPHTKGPQSAIVTGPAGQEIWTDKYGRVKVQFGWDRYGKNDE
                     NSSCWIRVSYPWAGKGFGMIQIPRIGQEVLVDFKNGDPDLPIIVGRTYNQDTMPPWGL
                     PGMASQSGIFSHSLQGGPTNGNMLRFDDKTGAEEVKFHAEKDLNTTVKNNETHTVMVD
                     RIKTIVKNETISVGEDRNATITKNDGLSVKLAQTINVGTTYRLDVGDQFTLRCGNAAL
                     VLHKDGSIEFCGKQLLLHTSDVMQLIGSGIDMNPDGGTAMTADDIAPLPTHDVSE"
     gene            3282..3727
                     /locus_tag="OO357_002289"
                     /pseudo
     CDS             3282..3727
                     /locus_tag="OO357_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233848.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DcrB-related protein"
     gene            3749..4156
                     /locus_tag="OO357_002290"
     CDS             3749..4156
                     /locus_tag="OO357_002290"
                     /inference="COORDINATES: protein motif:HMM:NF017315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAAR domain-containing protein"
                     /protein_id="HCR5887216.1"
                     /translation="MYEAARVDDPIYHTSALAGFLIGAIIGIAIIAVASFAFFTCGFL
                     AGLILSFLADQIASGVLQLGEAIGRSIHSTVGKILTGSDNVSTNSRPAARAVLSTVIC
                     EDHSPEIRIAQGSGNIYINSQLPVRMTTLSVMQ"
     gene            4153..6441
                     /locus_tag="OO357_002291"
     CDS             4153..6441
                     /locus_tag="OO357_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RHS repeat protein"
                     /protein_id="HCR5887217.1"
                     /translation="MIEDGSPNVFLGGGTQTVLEISPEIPDWLRQVVDVLYVVAGMLG
                     GLAGAWRQAAKMGSKFGTKCAAKFIGGEMVGMAISDTVMGLFSNPVDVTTGQKILLPE
                     TDFTLPGRLPVTCSRFYASHLETEGLLGRGWRLNWEINLREDETYITFIGVQGRELSY
                     PKEMLIPGHQIFDPEEQFYLSRLHDGCYVLHYTDCSYYVFDEFDDHGVAPLLFMETPY
                     RQRIAFGRENGRLVRVASSSGHHLLLHRTMTQAGERLSHIELLKGGRPGNLVEYRYDD
                     NGQLTGVVNRAGVTVRQFAYENGLMTEHRNATGFTCTYHWEEIEGFPRVVEHTTSDGE
                     DYRFHYDFAGGQTVVTGRPEQKWQWWFDEETYVTAHRTPGGGMYRFTYNENHFPVAVE
                     LPGERRVTLEYDTLSRVVKETDPAGRVTQTQWNGSFAEITRRALDDDHVWKADYNEHG
                     QVIRETDPEGRVTRYGYDDQGLPETVCHPGKQQDRYTWNALGLLSSHRRITGSVQSWQ
                     YTQRGMLARHTDEEKRETRWQYTPEGLVASLSNGNGAQYRFSYDGDGRLTGEQRPDGL
                     IRMFALNADGFPVIIPTQGTEGGVRNEQQERDALGRLLRSDTQHSTRTFSYNRLDQIT
                     EVTLTPTEEGERLHHMQADTVRFAYDRSGWLTAEHSVHGSIKYRRDALGNPTDITLPD
                     GQHLSHLYYGSGHLLQTALDGITVSEYERDSLHRQVIRTQGKLATFSGYNADNRLSWQ
                     RSLPGGSQNPQQAVLPRRRTTA"
     gene            6438..7427
                     /locus_tag="OO357_002292"
     CDS             6438..7427
                     /locus_tag="OO357_002292"
                     /inference="COORDINATES: protein motif:HMM:TIGR01643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887218.1"
                     /translation="MTARDYIWNADGEVGGINDKLRGCLVFSYDRSGWLTSRTGQMYD
                     HDHYYYDKAGNLLTDEYQGAVMDNRLPGYGRDRYRYNEWGELTERRDQQLEWNAQGQL
                     TRVISSNSETRYQYDALGRRISKATSNLHTDRGERSRTTFVWEGFRLLQETTWQGKRT
                     YLYDAEQPYTPVAAITGRGESQKIWYYHTDLTGTVHEVTAPDGTLVWAGYQAGFGENR
                     GDISNSGAYFEQPQRLPGQYFDEETGLHYNLFRYYAPECGRFVSQDPIGLNGGLNLYA
                     YAPNPLGWIDPLGLNSLGIDSNNIFRGDGNYKGGGIGQPKSGTISAQDIIDHL"
     gene            7527..7730
                     /locus_tag="OO357_002293"
     CDS             7527..7730
                     /locus_tag="OO357_002293"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887219.1"
                     /translation="MAVSDLDDLVKNNQITVLTQEKVKAIFGLESSKKIRKELGNILQ
                     NMARNNEVLVHGQILGNKVSRCN"
     gene            7748..8068
                     /locus_tag="OO357_002294"
     CDS             7748..8068
                     /locus_tag="OO357_002294"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887220.1"
                     /translation="MDEIRIRIAGNTAAPCFHVLKNKGYKVTMETHALSDDCLDCVYD
                     WYAEKNGQFFSATSPEELLGLVAMWEVRGNDWRANLNEINTYQKAIEESPFFDKNGNE
                     IESD"
     gene            complement(8134..8366)
                     /locus_tag="OO357_002295"
                     /pseudo
     CDS             complement(8134..8366)
                     /locus_tag="OO357_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000541009.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SymE family type I addiction module toxin"
     gene            complement(8476..9246)
                     /gene="yafV"
                     /locus_tag="OO357_002296"
     CDS             complement(8476..9246)
                     /gene="yafV"
                     /locus_tag="OO357_002296"
                     /EC_number="3.5.1.111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutaramate amidase"
                     /protein_id="HCR5887221.1"
                     /translation="MPSLKITLLQQPLVWVDGPANLRHFDRQLEGITGRDVIVLPEMF
                     TSGFAMEAAASSLAQDDVVNWMTAKAQQCNALIAGSVALQTESGSVNRFLLVEPGGTV
                     HFYDKRHLFRMADEHLHYKAGNARVIVEWRGWRILPLVCYDLRFPVWSRNLNDYDLAL
                     YVANWPAPRSLHWQALLTARAIENQAYVAGCNRVGSDGNGCHYRGDSRVINPQGEIIA
                     TADAHKATRIDAELSMVALREYREKFPAWRDADEFRLW"
     gene            9427..9873
                     /gene="ivy"
                     /locus_tag="OO357_002297"
     CDS             9427..9873
                     /gene="ivy"
                     /locus_tag="OO357_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308274.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family C-type lysozyme inhibitor"
                     /protein_id="HCR5887222.1"
                     /translation="MFKAITTVAALVIATSAMAQDDLTISSLAKGETTKAAFNQMVQG
                     HKLPAWVMKGGTYTPAQTVTLGDETYQVMSACKPHDCGSQRIAVMWSEKSNQMTGLFS
                     AIDEKTSQEKLTWLNVNDALSIDGKTVLFAALTGSLENHPDGFNFK"
     gene            complement(9916..12360)
                     /gene="fadE"
                     /locus_tag="OO357_002298"
     CDS             complement(9916..12360)
                     /gene="fadE"
                     /locus_tag="OO357_002298"
                     /EC_number="1.3.8.7"
                     /EC_number="1.3.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase FadE"
                     /protein_id="HCR5887223.1"
                     /translation="MMILSILATVVLLGALFYHRVSLFISSLILLAWTAALGVAGLWS
                     AWVLVPLTIILVPFNFAPMRKSMISAPVFRGFRKVMPPMSRTEKEAIDAGTTWWEGDL
                     FQGKPDWKKLHNYPQPRLTAEEQAFLDGPVEEACRMANDFQITHELADLPPELWAYLK
                     EHRFFAMIIKKEYGGLEFSAYAQSRVLQKLSGVSGILAITVGVPNSLGPGELLQHYGT
                     DEQKDHYLPRLARGQEIPCFALTSPEAGSDAGAIPDTGIVCMGEWQGQQVLGMRLTWN
                     KRYITLAPIATVLGLAFKLSDPEKLLGGAEDLGITCALIPTTTPGVEIGRRHFPLNVP
                     FQNGPTRGKDVFVPIDYIIGGPKMAGQGWRMLVECLSVGRGITLPSNSTGGVKSVALA
                     TGAYSHIRRQFKISIGKMEGIEEPLARIAGNAYVMDAAASLITYGIMLGEKPAVLSAI
                     VKYHCTHRGQQSIIDAMDITGGKGIMLGQSNFLARAYQGAPIAITVEGANILTRSMMI
                     FGQGAIRCHPYVLEEMEAAKNNDVNAFDKLLFKHIGHVGSNKVRSFWLGLTRGLTSST
                     PTGDATKRYYQHLNRLSANLALLSDVSMALLGGSLKRRERISARLGDILSQLYLASAV
                     LKRYDDEGRNEADLPLVHWGVQDALYQAEQAMDNLLQNFPNRVVAGLLNVVIFPTGRH
                     YLAPSDKLDHKMAKILQVPNATRSRIGRGQYLTPSEHNPVGLLEEALVDVIAADPIHQ
                     RICKELGKNLPFTRLDELAHNALTKGLIDKDEAAILVKAEESRLRSINVDDFDPEELA
                     TKPVKLPEKVRKVEAA"
     gene            12600..13178
                     /gene="lpcA"
                     /locus_tag="OO357_002299"
     CDS             12600..13178
                     /gene="lpcA"
                     /locus_tag="OO357_002299"
                     /EC_number="5.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-sedoheptulose 7-phosphate isomerase"
                     /protein_id="HCR5887224.1"
                     /translation="MYQDLIRNELNEAAETLANFLKDDANIHAIQRAAVLLADSFKAG
                     GKVLSCGNGGSHCDAMHFAEELTGRYRENRPGYPAIAISDVSHISCVGNDFGFNDIFS
                     RYVEAVGREGDVLLGISTSGNSANVIKAIAAAREKGMKVITLTGKDGGKMAGTADIEI
                     RVPHFGYADRIQEIHIKVIHILIQLIEKEMVK"
     gene            13384..14151
                     /locus_tag="OO357_002300"
     CDS             13384..14151
                     /locus_tag="OO357_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II glutamine amidotransferase"
                     /protein_id="HCR5887225.1"
                     /translation="MCELLGMSANVPTDICFSFTGLVQRGGGTGPHKDGWGITFYEGK
                     GCRTFKDPQPSFNSPIAKLVQDYPIKSCSVVAHIRQANRGEVALENTHPFTRELWGRN
                     WTYAHNGQLTGYKSLETGNFRPVGETDSEKAFCWLLHKLTQRYPRTPGNMAAVFKYIA
                     SLADELRAKGVFNMLLSDGRYVMAYCSTNLHWITRRAPFGVATLLDQDVEIDFSSQTT
                     PNDVVTVIATQPLTGNETWQKIMPGEWRLFCLGERVV"
     gene            complement(14122..14862)
                     /locus_tag="OO357_002301"
     CDS             complement(14122..14862)
                     /locus_tag="OO357_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein L,D-transpeptidase"
                     /protein_id="HCR5887226.1"
                     /translation="MRKIALILAMLLIPCVSFAGLLGSSSSTTPVSKEYKQQLMGSPV
                     YIQIFKEERTLDLYVKMGEQYQLLDSYKICKYSGGLGPKQRQGDFKSPEGFYSVQRNQ
                     LKPDSRYYKAINIGFPNAYDRAHGYEGKYLMIHGDCVSIGCYAMTNQGIDEIFQFVTG
                     ALVFGQPSVQVSIYPFRMTDANMKRHKYSNFKDFWEQLKPGYDYFEQTRKPPTVSVAN
                     GRYVVSKPLSHEVVQPQLASNYTLPEAK"
     gene            15246..15767
                     /locus_tag="OO357_002302"
                     /pseudo
     CDS             15246..15767
                     /locus_tag="OO357_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414762.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
     gene            15943..16440
                     /gene="rayT"
                     /locus_tag="OO357_002303"
     CDS             15943..16440
                     /gene="rayT"
                     /locus_tag="OO357_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="REP-associated tyrosine transposase RayT"
                     /protein_id="HCR5887227.1"
                     /translation="MSEYRRYYIKGGTWFFTVNLRNRRSHLLTTQFQTLRNAIINVKR
                     DRPFEINAWVVLPEHMHCIWTLPESDDDFSSRWREIKKQFTHACGLKNIWQPRFWEHA
                     IRNTKDYRHHVDYIYINPEKHGWVKQVSDWPFSTFHRDVARGLYPIDWAGDITDLSAG
                     ERIIL"
     gene            complement(16657..18369)
                     /locus_tag="OO357_002304"
                     /pseudo
     CDS             complement(16657..18369)
                     /locus_tag="OO357_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001140833.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            18341..19126
                     /gene="lafU"
                     /locus_tag="OO357_002305"
     CDS             18341..19126
                     /gene="lafU"
                     /locus_tag="OO357_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative lateral flagellar export/assembly
                     protein LafU"
                     /protein_id="HCR5887228.1"
                     /translation="MTTIKLIVNSVSKSERESIIAALHGQSIFNGGGLSPLNKISPSH
                     PPKPATVAVPEETEKKARDVNEKTALLKKKSATELGELATSINTIARDAHMEANLEME
                     IVPQGLRVLIKDDQNRNMFERGSAQIMPFFKTLLVELAPVFDSLYNKIIITGHTDAMA
                     YKNNIYNNWNLSGDRALSARRVLEEAGMPEDKVMQVSAMADQMLLDAKNPQSAGNRRI
                     EIMVLTKSASDTLYQYFGQHGDKVVQPLVQKLDKQQVLSQRMR"
     gene            19197..20252
                     /gene="dinB"
                     /locus_tag="OO357_002306"
     CDS             19197..20252
                     /gene="dinB"
                     /locus_tag="OO357_002306"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HCR5887229.1"
                     /translation="MRKIIHVDMDCFFAAVEMRDNPALRDIPIAIGGSRERRGVISTA
                     NYPARKFGVRSAMPTGMALKLCPHLTLLPGRFDAYKEASNHIREIFSRYTSRIEPLSL
                     DEAYLDVTDSVHCHGSATLIAQEIRQTIFNELQLTASAGVAPVKFLAKIASDMNKPNG
                     QFVITPAEVPAFLQTLPLEKIPGVGKVSAAKLEAMGLRTCGDVQKCDLVILLKRFGKF
                     GRILWERSQGIDERDVNSERLRKSVGVERTMAEDIHHWSECEAIIERLYPELERRLAK
                     VKPDLLIARQGVKLKFDDFQQTTQEHVWPRLNKADLIATARKTWDERRGGRGVRLVGL
                     HVTLLDPQMERQLVLGL"
     gene            20304..20597
                     /gene="yafN"
                     /locus_tag="OO357_002307"
     CDS             20304..20597
                     /gene="yafN"
                     /locus_tag="OO357_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system antitoxin YafN"
                     /protein_id="HCR5887230.1"
                     /translation="MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSAS
                     AFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRAKQYLNDMTDDDFNDFKE"
     gene            20600..20998
                     /gene="yafO"
                     /locus_tag="OO357_002308"
     CDS             20600..20998
                     /gene="yafO"
                     /locus_tag="OO357_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YafO"
                     /protein_id="HCR5887231.1"
                     /translation="MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALY
                     DDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRTNDEAHLVYCQGAFDEQAWLLIA
                     ILKPEPHKLARDNNQMHKIGKMAEAFRMRF"
     gene            21008..21460
                     /locus_tag="OO357_002309"
     CDS             21008..21460
                     /locus_tag="OO357_002309"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5887232.1"
                     /translation="MNNIQIRDYQPGDFQQLCAIFIRAVTMTVSQHYSLQQIAAWAQI
                     DESRWKEKLAKSQVRVAVINAKLVGFITCVEHYIDMLFVDPEYTRRGVASALLKPFIK
                     SESELTVDASITAKPFFERYGFQTVKQQRVECRGAWFINFYMRYKPQH"
     gene            21706..22749
                     /locus_tag="OO357_002310"
     CDS             21706..22749
                     /locus_tag="OO357_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5887233.1"
                     /translation="MGNYIRPLSDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVG
                     MPDLHPGRGYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSA
                     LDDVAEESWLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDA
                     QHLQLLVHSGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVK
                     ATGSPVLDVAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSE
                     ETLHSLAHGAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYK
                     SAESVVQCQGRPD"
     gene            22811..23425
                     /locus_tag="OO357_002311"
                     /pseudo
     CDS             22811..23425
                     /locus_tag="OO357_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(23482..24939)
                     /gene="pepD"
                     /locus_tag="OO357_002312"
     CDS             complement(23482..24939)
                     /gene="pepD"
                     /locus_tag="OO357_002312"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5887234.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            25201..25659
                     /gene="gpt"
                     /locus_tag="OO357_002313"
     CDS             25201..25659
                     /gene="gpt"
                     /locus_tag="OO357_002313"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5887235.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            25751..26995
                     /gene="frsA"
                     /locus_tag="OO357_002314"
     CDS             25751..26995
                     /gene="frsA"
                     /locus_tag="OO357_002314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase FrsA"
                     /protein_id="HCR5887236.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAATLYNIAAYPHLKGDDLAEQAQALSNRAYEEA
                     AQRLPGTMRQMEFTVPGGAPITGFLHMPKGDGPFPTVLMCGGLDAMQTDYYSLYERYF
                     APRGIAMLTIDMPSVGFSSKWKLTQDSSLLHQHVLKALPNVPWVDHTRVAAFGFRFGA
                     NVAVRLAYLESPRLKAVACLGPVVHTLLSDFKCQQQVPEMYLDVLASRLGMHDASDEA
                     LRVELNRYSLKVQGLLGRRCPTPMLSGYWKNDPFSPEEDSRLITSSSADGKLLEIPFN
                     PVYRNFDKGLQEITGWIEKRLC"
     gene            27053..27454
                     /gene="crl"
                     /locus_tag="OO357_002315"
     CDS             27053..27454
                     /gene="crl"
                     /locus_tag="OO357_002315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-binding protein Crl"
                     /protein_id="HCR5887237.1"
                     /translation="MTLPSGHPKSRLIKKFTALGPYIREGKCEDNRFFFDCLAVCVNV
                     KPAPEVREFWGWWMELEAQESRFTYSYQFGLFDKAGDWKSVPVKDTEVVERLEHTLRE
                     FHEKLRELLTTLNLKLEPANDFRDEPVKLTA"
     gene            complement(27493..28548)
                     /gene="phoE"
                     /locus_tag="OO357_002316"
     CDS             complement(27493..28548)
                     /gene="phoE"
                     /locus_tag="OO357_002316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoporin PhoE"
                     /protein_id="HCR5887238.1"
                     /translation="MKKSTLALVVMGIVASASVQAAEIYNKDGNKLDVYGKVKAMHYM
                     SDNASKDGDQSYIRFGFKGETQINDQLTGYGRWEAEFAGNKAESDTAQQKTRLAFAGL
                     KYKDLGSFDYGRNLGALYDVEAWTDMFPEFGGDSSAQTDNFMTKRASGLATYRNTDFF
                     GVIDGLNLTLQYQGKNENRDVKKQNGDGFGTSLTYDFGGSDFAISGAYTNSDRTNEQN
                     LQSRGTGKRAEAWATGLKYDANNIYLATFYSETRKMTPITGGFANKTQNFEAVAQYQF
                     DFGLRPSLGYVLSKGKDIEGIGDEDLVNYIDVGATYYFNKNMSAFVDYKINQLDSDNK
                     LNINNDDTVAVGMTYQF"
BASE COUNT         7076 a         7119 c         7605 g         6854 t
ORIGIN      
        1 atggcaatat cattatacat aaggatggta ggaggacgta tttattaaat caaaatactg
       61 gttcgattac ggctgtgata agatggaagt atgagtagta taaggataat accgttacag
      121 atgactggac tatatgggaa aaagctattt ttatgcgagc gatatattta tctgttcagc
      181 aggcatggaa tgggaaaata acttattctg tttccggtga atctgagttc gctaaaaaat
      241 ttcagggtaa agctttgcct tttgatgtaa gaataatttc tgcatctcag aatgaagact
      301 ggttggttat tgcaacaaag gttttgcctg gtgctgattt aagaacatat gttgatttta
      361 aaaacagtac ggttcatgtt gattcagcgg gcctggaaaa agtagcgaaa tgtattaact
      421 gtaataatac gttgcaagta aatatacctc atgaagccgg acatgtactt ggttacttag
      481 atgatgatta tgattcttcg tctccgtatg ttggagatat tagtgggttg atgaatgtgg
      541 gaatggagct ttgggaacga taccttaaaa acgcaactat aacgttgaat atcataatgc
      601 ctgaaactaa atttacttta ttgaatgtaa ccaaatgaaa tttttctgta ttatagtgat
      661 tttcctttca ataaattaca tagctatagt gaatgttttc aagtcttctg cagttgctca
      721 atccaccaaa tattatgaag aaaatgatat acatgatgga agtaatgaca tcttttttat
      781 gaagaaatat gggccgcctg ctcatgagtt cgtgaggcca cgatataaat ttgttggtga
      841 gtattatatc ggcttgaatc aaacttatcg cataacagat cctgaatttc aagatgttcc
      901 tattaaggaa atgttctgga atttgcaggg ggatttgaat ctgacatgtt ggtttcatta
      961 taaagacggg caatggagag tgattactta tattttttgg ccaccagggg ctagatttta
     1021 atccggtatt aaaggagcaa atatcatgag ttttgtatct acgggtaata aaccagttgg
     1081 aaacggtgga cccgtcatca ccacaccacc aatagctggc gaaagtggcg gtatgagcac
     1141 aggttcagct gtaaccgatg tatctggtgc cgccgaggag atggcagaac aggctgcggc
     1201 ggacctgttt ggtgcgctgc cggaaccgtc aggtctggtt aaagccgcag tagctgctgc
     1261 gcaggctgcc gctgcagcag cagggatctc ggatatggct ggtgcagttc aggatgcagc
     1321 ggcgtcgctt gcagccggtg ctccgggggc gcataacgtc acagtaagtg gtagtgcggt
     1381 gccgccgcag atgcttttgt ttgcaggcat gaatggttcg gaagggcttg ggaatctgtt
     1441 ttcttacaca gtacagctta aaacgctgga cgccctgaac ctggggtacg tttcccccgc
     1501 tgctaacctg ccgcttcagc tcatggtggg caaagacctg tgcgtcagta tcgaactgga
     1561 cggcggcgga aaacggtata tcagcgggct ggtgacggcg gcgagggtgg tcggccatga
     1621 gagccgttcg gtaacgtatg aattacgcat tgagccatgg ctgaagctgc tgacccatac
     1681 cagcgactac aaagcgttcc agaacaaaac cgtggtggat attctcgatg aagtactgga
     1741 tgaatattcc tttccggtgg aaaagcggct ggtggaaaat tatccacccc gcgcctggca
     1801 ggtgcagtat ggtgaaaccg acttcgattt catccagcgc ctgatgcagg aatgggggat
     1861 ttactggtgg tttgagcaca gcgagaacag ccacacgctg gtgctggtgg atgccatcaa
     1921 tggtcataaa gcgtgccctg actcgccgct ggtggagtgg caccaggaag ggctgaaact
     1981 ggataaggaa tttatccaca ccattacggc cagtgagagg ctgcgtactg gaaagtgggt
     2041 gatggacgat tttgatttta tgaagcctcg ttcgctgctt aaaagtaccg tggccagtcc
     2101 gcgtgatacc ggtcatgctg aatacgagca ttacgaatgg cctggtgatt acttcactac
     2161 gggcgaaggc gagatgctga cgcgcattcg tatggaagcg cagcgcagcc caggcagccg
     2221 ggcacatggg gccggtcata tccgtaccct catgaccggg tacaccttta cgttgatgaa
     2281 tcacccgacg gcagaaatta atcaggaata tctgctggta cagaccactc tgtttttacg
     2341 ggataatgcg cagcacagcg gtcaggatca gcattttacc tatgtcacca cgtttgaact
     2401 gcaccccacc agcgaagttt accgcccaca acgaaccctg agcaaacccc acaccaaagg
     2461 cccgcagagc gccatcgtca ccggcccggc ggggcaggaa atctggacgg ataaatatgg
     2521 ccgggtgaag gtgcagtttg gctgggaccg ttacggaaag aatgacgaaa acagttcctg
     2581 ctggatccgc gtcagctatc cgtgggcggg taaaggattc ggcatgatcc agatcccgcg
     2641 tattggccag gaagtactgg tggatttcaa aaacggcgat ccggatttgc ccatcatcgt
     2701 ggggcgcacc tataaccagg acaccatgcc gccgtggggg ctgccgggca tggcgtcgca
     2761 gagtgggata ttcagccact cattgcaggg agggccgact aacggaaata tgctgcgctt
     2821 cgatgataaa acgggtgcgg aagaggtgaa gttccatgcg gaaaaagacc tcaataccac
     2881 ggtaaaaaac aatgaaaccc acacggtcat ggtcgatcgc ataaaaacca ttgtcaaaaa
     2941 tgaaaccatc agcgtgggag aagaccgtaa tgccaccatc acaaaaaatg atggtctttc
     3001 cgtcaaactg gctcaaacca tcaatgtcgg aacgacatat cgtctggatg ttggcgatca
     3061 atttaccctt cgttgcggca atgcagcgct tgtattacac aaggatggct cgattgagtt
     3121 ttgcggaaaa caactgttac tgcacaccag cgatgttatg caactcattg gcagtggcat
     3181 tgatatgaat ccggatggcg gcacagccat gactgcggat gatattgccc cgttacctac
     3241 ccacgacgtt tctgaataaa aaatataaaa ggaatttttc tatgaccagc ctttaccgta
     3301 ttcaggaggg ctgctttgcc ctgcctgaaa cttttcttga tcgcaccgtc aatatcttcg
     3361 tcccatcggg caatgaaagg gcaactccca gtctgaatat tttccgcgat acgctcaggc
     3421 cagatgaaaa tctgaccacc tatattgacc gccagatagc cttgatgaaa aaaaaaacct
     3481 aggccagcac cgggtacttt cgcggggaac ggcactggcg ggaactggcg aagctgccat
     3541 gacaggtgaa cagattctcg caactcatcg ttctggaaaa acagaagtgt accagcgtca
     3601 ggccgggttt atcaccgggc cagcaaaggt actaatgttg acgctgacca ctcagcgtcc
     3661 ttttgatgat cacaccgacc agctatggac tgcatggttg actagttttc agcctgctaa
     3721 aagctaaaaa aaacagggag gttttgtcat gtatgaagcc gcacgggtgg atgaccccat
     3781 ctaccacacc agcgcccttg ccgggttttt gataggggcc atcataggta tcgccattat
     3841 tgccgtcgcc agctttgctt tttttacctg cggctttttg gccgggctga ttttgagttt
     3901 tctggctgat cagatagctt ccggagtcct gcaactgggt gaggctattg ggcgctcgat
     3961 acattccaca gtgggaaaaa ttctgaccgg ttctgataat gtcagcacca acagtcgccc
     4021 ggccgcccgg gccgtactga gtacggtgat atgcgaagac cattccccgg aaatccgcat
     4081 tgcccaaggt tccggcaaca tctacatcaa cagccagctg cccgtaagga tgaccacact
     4141 gagtgtgatg cagtgattga ggacggctcc cccaatgtct ttctcggcgg cggtacgcag
     4201 acggtactgg aaatcagccc ggaaatccct gactggctgc gtcaggtggt ggatgtgctg
     4261 tatgtggtcg ccggaatgct tggcgggctg gctggcgcct ggcgtcaggc ggcaaagatg
     4321 gggtcgaaat ttggtacaaa atgtgcggca aaatttattg gtggcgaaat ggtcgggatg
     4381 gccatctctg acacagtcat gggattgttc agcaatccgg tagatgtcac cactgggcaa
     4441 aaaattttgc tgccggagac ggattttacc ctgccaggac gcctgcctgt cacctgttcg
     4501 cgcttttacg ccagccacct ggaaactgaa ggattgctgg gcagaggatg gcggctgaac
     4561 tgggagataa acctgcgtga ggatgaaaca tatatcacct tcatcggtgt acagggtcgg
     4621 gaactgagtt atccgaaaga gatgctgata ccgggccacc agatattcga cccggaagag
     4681 cagttttacc tgagccgtct gcatgacgga tgctatgtgc tgcattacac cgactgcagc
     4741 tattacgtgt ttgatgaatt tgatgaccac ggcgtcgcgc cgttactttt tatggagacg
     4801 ccgtaccggc agcgtattgc tttcgggcgt gagaacggca ggctggtgcg ggtggcctcc
     4861 agcagcggac accatctgtt attgcaccgc acgatgacgc aagcagggga acgcctgtcg
     4921 catattgaac ttttgaaggg cgggcgtccc ggtaacctgg tggaatatcg ttacgacgat
     4981 aacgggcaac tgactggcgt ggtgaaccgg gctggtgtga cggtgcgcca gtttgcctat
     5041 gaaaatggcc tgatgacaga gcatcgcaac gcgacgggct ttacctgtac ctaccactgg
     5101 gaagagattg aaggtttccc gcgcgtggtg gaacacacga ccagcgacgg cgaggattac
     5161 cggttccact atgattttgc tggcgggcag acggtggtaa ccggcaggcc ggagcagaag
     5221 tggcagtggt ggtttgatga agagacgtat gtgactgcgc accggacgcc gggcggcgga
     5281 atgtaccgct tcacgtacaa cgaaaaccat tttccggtag cggtggaact ccccggcgaa
     5341 cgcagggtaa cgctggaata cgacaccctg agccgggtgg tgaaagagac ggacccggca
     5401 ggccgggtga cacagacgca gtggaacggc agttttgctg aaatcacgcg ccgggcgctg
     5461 gatgacgacc acgtatggaa ggcggattac aacgaacacg gccaggtgat acgggagacg
     5521 gacccggaag ggcgcgtcac gcggtacggg tatgatgatc aggggctgcc ggagacggtc
     5581 tgccaccccg gtaaacagca ggaccgttac acctggaacg cgctggggtt actgagcagc
     5641 caccgtcgca tcacgggcag tgtgcagtcc tggcagtata cgcagcgcgg gatgctggcg
     5701 cgccatacgg atgaagagaa acgggagacg cgctggcagt acaccccgga agggctggtt
     5761 gccagtctga gcaacggcaa cggggctcag taccggttca gctacgacgg cgacggacgg
     5821 ctgacgggag aacagcgccc ggacggcctg ataaggatgt ttgcgctgaa tgcggacggg
     5881 tttccggtca tcatcccgac gcagggcaca gagggcgggg tgcgcaatga acagcaggag
     5941 cgtgatgcgc tgggccgcct gctgcgcagt gacacgcagc acagtacacg gaccttcagc
     6001 tacaaccgtc ttgaccagat aacggaagtg acgctcaccc ccacggaaga aggggaacgg
     6061 ctgcaccaca tgcaggcgga cacggtgcgt tttgcgtatg accgcagcgg gtggctgacg
     6121 gcagagcaca gcgtgcacgg cagtatcaaa taccggcgcg atgcgctggg caatcccacc
     6181 gatatcacgt tgccggacgg gcagcatctc agtcatcttt actacggcag cgggcatctg
     6241 ctccagacgg cactggacgg tatcacggtc agtgaatatg agcgtgacag cctgcaccgt
     6301 caggtgatac gcacgcaggg gaaactggcg acgttcagcg gctacaacgc cgacaaccgg
     6361 ttaagctggc agcgcagcct gccaggtggc agccagaatc cacagcaggc ggtactcccc
     6421 cggcgcagaa cgacagcgtg acggcgcggg actatatctg gaacgcagac ggtgaagtcg
     6481 gcggcatcaa tgacaaactg cgcggttgcc tggtgttcag ctatgaccgc agcggctggc
     6541 tgaccagccg cacggggcag atgtatgacc atgaccatta ttattacgac aaagcgggca
     6601 acctgctgac ggatgagtat cagggagcgg tgatggataa ccgcctgccg ggttacgggc
     6661 gtgaccgcta ccgctataac gaatggggcg aactgacaga gcgcagggac cagcagctgg
     6721 agtggaacgc gcagggccag ttaacgcggg tcatcagcag caacagcgag acgcggtatc
     6781 agtacgatgc gctgggcagg cgcatcagca aggcgaccag caatctccat accgaccggg
     6841 gagaacgaag ccggacaacg tttgtgtggg aagggttccg gctgttacag gagacaacgt
     6901 ggcagggaaa gcggacatac ctgtacgatg cggaacagcc gtacacgccg gtggctgcca
     6961 tcacggggcg cggagagagc cagaaaatct ggtattacca cacggacctg acgggcacgg
     7021 tacacgaggt aaccgcgccg gacgggacgc tggtgtgggc gggctaccag gcgggatttg
     7081 gtgaaaaccg gggcgacatc agcaacagcg gggcgtactt tgagcagccg cagcggctgc
     7141 cggggcagta ctttgacgaa gaaacagggc tgcattacaa tctgttcagg tattatgcgc
     7201 cggagtgcgg gaggttcgta agccaggacc cgataggatt aaacggtggg ttgaacttat
     7261 atgcttatgc gcctaatccg ttaggttgga tcgatccgct aggactgaat tctttaggaa
     7321 tagattcaaa taatatattt agaggagatg gtaactataa aggtggcgga attggacagc
     7381 caaagagtgg cacaatatca gcacaggaca ttattgatca tctctaagaa aaaggtgatg
     7441 ggcgtttaac atcttttagt actaaaaaaa catctagttc caactctcgt ggtgcagatt
     7501 ttttctcaaa aaaacatgtc ttcaaagtgg cagtttctga tctcgatgat ttagttaaaa
     7561 ataatcagat cactgtttta acacaagaaa aagtaaaagc aatatttggt ttagaatcaa
     7621 gcaagaaaat tagaaaagaa cttggtaata ttcttcagaa tatggctagg aataatgagg
     7681 tgttagttca tgggcaaatt cttggaaata aagttagccg atgtaattag ttagtggaga
     7741 ggaagttgtg gacgaaatta gaataagaat tgctggtaat acggctgcgc cttgtttcca
     7801 tgtgctaaaa aataagggat ataaagtaac aatggaaact catgcattat ctgacgattg
     7861 tttagattgt gtatatgatt ggtatgcgga aaaaaatgga caatttttta gtgcaacgag
     7921 tccagaagag ttacttggat tagttgcaat gtgggaagtt cgtggtaatg actggagagc
     7981 taatttgaat gagataaata cttatcaaaa agccatagaa gaatccccgt tttttgataa
     8041 aaacggcaat gaaatagagt ctgactaaat tttattgata tagcaacgat aataaccgga
     8101 aatttcttcc agatctttcc ggctttttat tatttaactt tcgttcagca cactaaacat
     8161 cccgtctcgc atccccttaa ctggctgttt aacctgataa agttccctgc gcagttcctg
     8221 tacttcgttg ttatcccaag caggatcaaa catccctccg agatcttcac ggtaacgccc
     8281 gtgccgatct caaatcccgc ttttctcagc catttggcgc tgattttgat caccggcgtg
     8341 tcgctgcggt tgggcagata acccacggta cagcgacgct gtgttttgga aatggtgact
     8401 tctggcgtaa tattgtgttt aagttagggg ctttcgtaat ctagcccagc ccccggccct
     8461 aaacacaacc cccactcacc acaacctaaa ctcatccgca tcccgccatg ccggaaactt
     8521 ttctctatat tcccgcaatg ccaccatcga cagctccgca tcaatacgcg ttgccttatg
     8581 cgcgtcggcg gtagcgataa tctcgccttg cggattaatc acccggctgt caccgcgata
     8641 atggcagccg ttgccatcgc tgccgacgcg attgcatccc gccacatacg cctgattctc
     8701 aatcgcgcgg gccgtcagca atgcctgcca gtgcagagag cgcggagctg gccagttggc
     8761 gacgtacagg gcgaggtcat aatcgttgag attgcgcgac cacactggaa aacgtaagtc
     8821 gtagcacacc agcggcaaaa tacgccagcc gcgccattcc acaatcactc gcgcattgcc
     8881 cgctttataa tgcagatgct catctgccat gcggaacaga tgacgcttat cataaaaatg
     8941 taccgtgccg cccggctcaa ccagcaaaaa gcggttaacc gaaccagact ccgtttgtaa
     9001 tgcaacactg cctgcaatca gcgcattgca ctgctgcgcc ttagctgtca tccagttcac
     9061 tacgtcatct tgtgcaagcg acgaagctgc cgcttccatg gcaaagccgc tggtaaacat
     9121 ctccggtaga acgatcacat cgcgcccggt aataccttcc agttgacgat caaaatggcg
     9181 caggttggca ggaccatcca cccacaccaa cggttgctgc aaaagcgtaa tcttcaaact
     9241 aggcacggtg tacaactcct ttatgcgaag ggttttataa ctttaacacc ttatcaggca
     9301 gttgccttag cgcagaataa attgataaca aatgctgata ttggaaatat ctgatttgca
     9361 aattatcgtg ttatcgctag gctttaggag gttaataaca tgggcaggat aagctcggga
     9421 ggaatgatgt ttaaggcaat aacgacagtc gccgcactgg tcatcgccac cagtgcaatg
     9481 gcgcaggatg atttaaccat tagcagcctt gcaaagggcg aaaccaccaa agctgcgttt
     9541 aatcagatgg tgcaagggca taagctacct gcatgggtga tgaaaggcgg tacttatact
     9601 cccgcacaaa ccgtaacgtt gggagatgag acgtatcagg tgatgagcgc gtgcaaaccg
     9661 catgactgtg gctcgcaacg tatcgctgtg atgtggtccg agaaatctaa tcagatgacg
     9721 gggctgttct cggctattga tgagaaaacg tcgcaagaga aactcacctg gctgaatgtg
     9781 aacgatgcgc tttcgattga tggtaaaacg gtgctgttcg cggcgttgac cggcagcctg
     9841 gaaaaccatc cggatggctt taattttaaa taattagcgg ataaagaaac ggagcctttc
     9901 ggctccgtta ttcaattacg cggcttcaac tttccgcact ttctccggca actttaccgg
     9961 cttcgtcgcc agctcttccg gatcaaagtc atcaacgtta atactgcgca gacggctttc
    10021 ttccgctttc accagaatag cggcttcatc tttatcaatc agccccttcg tcagcgcgtt
    10081 gtgcgccagt tcatccagac gggtaaatgg cagattttta cccagctctt tacagatccg
    10141 ctgatgaatc gggtcggcgg caatcacatc caccaacgcc tcttccagta aaccaaccgg
    10201 gttatgctcg cttggcgtca ggtactgacc gcgaccaata cgtgaacgag tggcgttcgg
    10261 cacttgtaaa atcttcgcca ttttatgatc cagcttgtca gacggtgcca gataatgacg
    10321 tccggtcggg aagatcacca cattcagcag cccggcaacc acgcggttcg ggaagttttg
    10381 cagtaaattg tccattgcct gttctgcctg atacagcgca tcctgtacgc cccagtgcac
    10441 cagcggcaga tcggcttcat tacggccttc gtcgtcataa cgcttcagaa cggcagaggc
    10501 gaggtaaagc tggcttaaaa tatcccccag acgtgccgag atacgctcac gacgtttcag
    10561 gctgccgccc agcagtgcca tcgagacatc agaaagcagg gcgaggttgg cgctcaggcg
    10621 gttcaggtgc tgatagtagc gtttagtggc atcgccggtt ggcgtgctgc tggttaaacc
    10681 gcgcgtcagg cccagccaga agctgcgaac tttgttgcta ccgacgtgac cgatatgttt
    10741 gaacaacagt ttatcgaacg cgttgacgtc attgttcttc gccgcttcca tctcttccag
    10801 cacgtacgga tggcaacgaa tcgctccttg tccgaagatc atcatgctgc gggtcagaat
    10861 gttagcccct tcaacggtga tggcaatcgg tgcgccctgg taagcacgcg ccaggaagtt
    10921 gctttgcccg agcataatgc ctttaccgcc ggtaatatcc atcgcatcaa taatcgactg
    10981 ctgcccgcgg tgggtacagt gatacttaac gatagccgac agcacggcag gtttttcgcc
    11041 gagcataatg ccgtaggtaa tcagcgatgc cgcagcatcc attacataag cgttcccggc
    11101 aatacgcgcc agcggctctt caatcccttc catcttaccg atagagattt tgaactgacg
    11161 gcgaatgtga gaatacgcgc ccgttgccag cgctaccgat ttcacgccgc cggttgagtt
    11221 ggaaggcagg gtgatgccgc ggcctaccga gaggcactcc accagcatcc gccagccttg
    11281 cccggccatt ttcggcccgc cgatgatgta atcgatcggc acgaagacat ctttaccgcg
    11341 cgtcggtccg ttctggaacg gtacgttcag cgggaagtgg cgacgaccaa tttccacgcc
    11401 cggcgtggtg gttgggatca gcgcacaggt aatgcctaaa tcttctgcac cgccgagtaa
    11461 tttttccggg tcggagagtt taaacgccag cccaagcacg gtcgcaatcg gtgccagcgt
    11521 aatgtagcgt ttgttccagg tcagacgcat ccccagcacc tgctggccct gccattcgcc
    11581 catgcagaca atcccggtgt ccggaatcgc gcccgcatcg gaacccgctt ccgggctggt
    11641 cagtgcaaag caggggatct cctgaccacg cgccagacgc ggcagatagt gatctttctg
    11701 ctcgtcagtg ccgtaatgtt gcaacagttc gcccgggcct aatgagtttg gcacgccgac
    11761 ggtaatcgcc aggatcccgc tcacgccaga gagtttttgc agcacacgag actgggcata
    11821 agctgagaac tccagtccgc cgtactcttt tttgataatc atcgcgaaga agcgatgctc
    11881 tttaaggtac gcccacaact ccggtggcag atccgccagc tcatgagtaa tctggaaatc
    11941 attcgccatc cggcaggctt cttctaccgg gccgtcgaga aacgcttgct cttcagcggt
    12001 caggcgcggc tgcggatagt tatgcagctt tttccagtcc ggcttgccct ggaacaagtc
    12061 gccctcccac caggtggtgc ccgcatcaat cgcttctttc tcagtgcgcg acatcggcgg
    12121 catcacctta cggaaaccgc gaaataccgg cgcggaaatc atcgacttac gcataggcgc
    12181 aaagttaaat ggcacgagga taatggtcag aggcaccagt acccacgccg accacagacc
    12241 agcaacgccg agggcggctg tccaggcgag caaaatcaga ctgctgataa ataagctcac
    12301 gcggtgatag aacaacgcgc cgagcaggac aaccgtagcg agaatactca aaatcatcat
    12361 aacgaaaagc cccttacttg taggaggtct gaccacttgt gatgatatgg ttgtagtgga
    12421 tgtaaaaaca tttagcaatg tgtttacaat ataattacaa caaagctcac attgttgctg
    12481 tttttatccg cacttcaggt caaaaagtcc tggtcatagc acctgcccgt acttctcgct
    12541 tttggcggta tccggtacac tgcattttgt ctattacatt tatgctgaag gatatcctca
    12601 tgtaccagga tcttattcgt aacgaactga acgaagcggc ggaaacgctg gctaactttt
    12661 taaaagatga cgccaatatt cacgccattc agcgcgcggc ggtcctgtta gcagacagct
    12721 ttaaagccgg tggcaaagtg ctttcctgcg gcaacggcgg ttcccattgc gacgctatgc
    12781 actttgccga agagttgacc ggtcgctacc gtgaaaaccg tccgggctac ccggcgattg
    12841 ctatttctga cgttagtcat atttcctgcg tcggtaatga tttcggtttc aatgatattt
    12901 tctcccgcta cgttgaagcg gtaggtcgcg aaggcgatgt actgctgggg atctccacct
    12961 ccggtaactc tgcaaacgtg atcaaagcga tcgcagcggc gcgtgagaag gggatgaaag
    13021 tgatcaccct gaccggtaaa gacggcggta aaatggctgg cacggcggat atcgaaattc
    13081 gcgttccgca ctttggttat gccgaccgca ttcaggagat tcacattaaa gtgatccata
    13141 tcctgatcca gttaattgaa aaagagatgg ttaagtaagt ctggcgtagg ccggataagg
    13201 cgtttacgcc gcatccggca cttgtgcgct gatgcctgat gcgatgctga cgcgtcttat
    13261 catgcctaca aatctgtacc cgaaccgtag gccgaataat tcgtttacgc cgcatccgac
    13321 ctgaaaattc ttaaatcaat cttcgccggg gtccatgcgc tcccgctgtt gtggaggtta
    13381 cccatgtgcg aattgctcgg gatgagcgcc aacgtcccta ccgatatctg ctttagtttc
    13441 accggacttg tacagcgtgg tggtggaacc gggccacata aagatggctg gggcattacc
    13501 ttttacgaag gtaaaggctg tcgcacattt aaagatccac aacccagctt taattccccc
    13561 atcgccaaac ttgtccagga ctacccgata aaatcctgtt cggtggtggc tcatattcgc
    13621 caggctaatc ggggcgaggt ggcgctggaa aatactcacc catttacccg cgagttatgg
    13681 gggcgtaact ggacttatgc ccataacgga caactgacgg gctacaaatc actggaaacc
    13741 ggcaacttcc gcccggtcgg tgaaaccgac agcgaaaaag ccttttgctg gctgctgcat
    13801 aaattaacgc agcgttaccc gcgcacgccg ggcaacatgg cggcagtgtt taaatatatc
    13861 gcctcactgg cggatgaact gcgggcgaag ggcgttttca acatgctgct ttcggacggg
    13921 cgctatgtaa tggcgtattg ctcgactaat ttacactgga tcacccgccg cgcgccgttt
    13981 ggcgtggcaa cgttgctgga tcaggatgtg gaaatcgact tcagctcgca gaccacaccg
    14041 aatgatgtgg tcacggtgat tgcgacacag ccgctgacgg gcaatgaaac ctggcaaaaa
    14101 attatgccag gcgaatggcg cttattttgc ctcggggagc gtgtagtttg atgccagttg
    14161 tggctgcacc acttcgtggc ttaacggctt gctgaccacg taacggccat tggccacaga
    14221 aacggtcggt ggcttacggg tttgctcaaa gtagtcgtag cccggcttca gctgctccca
    14281 gaagtcctta aagttggaat atttatggcg cttcatattg gcgtcggtca tgcggaatgg
    14341 gtaaatactc acctgcacgc tcggctgacc aaacaccagc gcaccagtaa cgaactggaa
    14401 gatctcatca ataccctgat tggtcattgc gtagcagccg atggatacac aatcgccgtg
    14461 gatcatcagg tatttccctt cataaccatg cgcacggtca taagcattgg ggaatccaat
    14521 attaatcgct ttgtagtaac ggctgtctgg ttttaactga ttacgctgga cgctataaaa
    14581 cccttccggg cttttgaaat cgccctgacg ctgttttggc cctaagccgc cggaatattt
    14641 acagatttta taactgtcga gcagttgata ttgctcgccc attttgacgt aaagatcgag
    14701 cgtacgttct tccttgaaga tctggatgta gacaggggat cccatcaact gctgcttata
    14761 ctctttgctc actggcgtgg tcgagctact gctgcccagc aaaccggcaa acgaaacgca
    14821 cgggatcaac aacatcgcaa gaattaatgc gattttacgc atactgctta ttccttgata
    14881 aaacggttac acacgccagg acggcaaaat ggatcccaaa tcggaatagt ctggatttgg
    14941 aaggctcaca ttatcaccaa aagagtttta cgcaagcctg tcggcgctgg gtttacaaat
    15001 ttagcacagg ggcagttttc aatagtccga aaagcgcatg tatcatttat ttcctaatat
    15061 tatgaaatct caatagtaat atgcaaataa gcaattaata atcccgttta ctcctccggt
    15121 cttttaaaat tctcaccttc attcagccgg tttatgtctt tcatgtcttc gttcttgctc
    15181 ggcagatttt tgcatccagg cgtgttttcg ttatgcgttt tgctcccttt actcgccagc
    15241 gccaccacaa ccgggagcag ataaaacagc atccagaatg gtttcctgcg ccgctcaagg
    15301 cgagtgacag acgctggcag gcgctggcgg aaaacaacca ctttttaagc agcgatcacc
    15361 tgcataacat caccgaagtg gcgattcacc gcctggagca gcagcttggc aagccttacg
    15421 tctggggcgg cacgcggccc gatcaaggct tcgattgtag tgggttagtt ttttatgcct
    15481 acaacaagat ccttgaggcg aaactcccgc gcacggccaa tgagatgtac cactaccgtc
    15541 gggcaacgat tgtggcgaat aacgacctgc gccggggaga tttgctattt ttccatatcc
    15601 acagccgcga gattgccgac catatggggg tgtatctggg cgacggtcag tttattgagt
    15661 cactgcgcac cggggaaacc attcgggtaa gccggttagc cgaacctttc tggcaggacc
    15721 attttttagg cgcgcgcagg attttgacgg aagagatgat tttgtaggcc ggataaggcg
    15781 tttacgccgc atccggcggt tgtgcacgga tgcctgatgc gacgctgacg cgtcttatca
    15841 tgcctacaaa tctgtgcgtt tttgcgagcc gctttcccga tataacacag cgtcattgtc
    15901 ggcctttcct tttcctctta ccgttgagaa aaaggagtcg ctatgtcgga atatcgtcgt
    15961 tattacatca aggggggaac atggtttttc acggtaaatt tacgcaatcg tcgaagccat
    16021 cttctgacca cccagttcca gacgctccgt aacgccatta ttaacgttaa gcgagacaga
    16081 ccttttgaaa ttaacgcctg ggtcgttttg ccggagcata tgcattgcat atggacatta
    16141 cccgaaagcg atgatgattt ttcctctcgc tggcgggaga ttaaaaagca gttcacccat
    16201 gcttgcggat tgaaaaatat ctggcaaccg cgtttttggg agcatgccat tcgcaataca
    16261 aaagattatc gccatcatgt tgattatatt tatataaatc cagagaaaca tggctgggta
    16321 aagcaggtga gtgattggcc attctcaacg ttccatcgcg atgtcgcgcg agggttatat
    16381 cccatcgatt gggcgggaga cataacggat ttaagtgccg gggagcgtat cattttataa
    16441 ctgtgcgctg gtgccggatg cgcttacgct tatccggcct acaaatcgtg ctttaacggt
    16501 aggccggata aggcgttcac gccgcatccg gcagttatgc gcagatgcct gatgcgacgc
    16561 tggcgcgtct tatcatgcct aaaaacttgt gcctgaacgg taggccggat aaggcgttta
    16621 cgccgcatcc ggcaattgtg caccgatgcc ggattcttac atcaacgccc ccataatctt
    16681 caactccagc tcatccggca cttcgttata cgacagcaca tgcagccccg gcgcaaacaa
    16741 ccttgcataa cgcggcagca aagggcgtag ctgcggcggc accagcagca ccggatcttt
    16801 ccccgccgct ttcatctgct ctttcacctg tggcatcgtg ctctggaact ggttgagcat
    16861 attcgggtca accggcacgc tgtcgagcat cactttgccg gcctgttgcg cctgattcac
    16921 tacgttggtc agcagatttt ccagctcatt attcagcgta tacaccgtca gctcctgctt
    16981 gcgaacgaac ggatgagtaa tgctgcgccg cagcgccagg cgcacatcgg ccgccagcag
    17041 aatatgatct ttagtcaccg tgctactggc gaccagcacg gtggcgatgg tgacgatatc
    17101 gcgcagggaa acgccttcgg tcagcagcgc ccggtacact ttcagcaatt ggctgtaatt
    17161 gagcgccgcg cttaaatcct ccgccaggcg cggtgccatc gacgacaaac ggttatgcaa
    17221 ctgcgtgatg tcatcatagt taaacaaatc aggaatatag ctgcgcacaa tcttgttcac
    17281 atgggtggca atcacactgg cgctgtcgat cacctgatac cccatattca gagccttcgc
    17341 tttctgcgca ggctggatcc aggtgaccgg tatcccgtac gccgggtcgt tccccagcac
    17401 gccgtcaatc tcgccgtagg tttcgctgga gggcagcgcc atcagtttat ccgccggaat
    17461 atccgcttca tcagcctcaa tgccgttgat gaaaatagcg tactggctgg gcttgaggcg
    17521 gaagttttcc cgaatgcgga tctccggcag cagcacgccg ttaccgtcgg aaatcacctg
    17581 ccgaacgccg cgaatccgct gggtgagcgg gttgccctgc gctttgtcca ccagcgccac
    17641 cagcttgtag ccgaggctta agctgatggg ctcgatcagc ggaatagttt cccaactgac
    17701 ctgttgttcg ctggtttcag tgatggtgcg ggtcagcgtt tcgaggcttt tctcttccgc
    17761 ctccgccgcc tgcgggcgtt tgctcatccg ccagccggta aagccaagca gggtgctgaa
    17821 cagcaaaaac ggcagatgcg gcatccccgg caccaccgcc agcacgaaca tgatcccggt
    17881 ggcggtgtag agcaccgacg ggcttgccag caactggtgg cgcacgtcat gggcgatatc
    17941 gccgttgtcg ctgacgcggg tgacgataat cgccgccgcg gtggagagca gcagggaagg
    18001 gatctgcgcc accaggccgt cgccgatggt catcagcacg tattgctgga aggcggcgtc
    18061 ggcgctcagg ttgtatttga agatgccgat acagacgccg ccgctcaggt tgatcgccag
    18121 aatcatcatc ccggcgatgg cgtctccgcg tacaaacttc gatgccccgt ccatcgcgcc
    18181 gtagaaatcg gcctcgctgg caacatcttt acgccgggtt tgcgcctgcg cctggttgat
    18241 caatccggcg ttaagatcgg cgtcaatcgc catctgtttg ccgggcatcg cgtccagggt
    18301 aaagcgggca gaaacctcgg aaatacgctc ggcctctttg gtgacgacga taaagttgat
    18361 tgtcaacagc gtcagtaaat ccgagcgtga aagcattatc gccgcgctgc acgggcagtc
    18421 gatttttaac ggtggtgggc tgtcgccgtt aaataaaatc agcccgtctc atccgccaaa
    18481 acctgccacc gtggcggtgc cggaagagac ggagaaaaaa gcgcgtgatg tgaatgagaa
    18541 aacggcgctg ctgaagaaga aatccgccac cgagcttggc gagttggcaa ccagtatcaa
    18601 caccattgcc cgtgatgcgc atatggaagc gaatctggag atggagattg ttccccaggg
    18661 attacgcgtg ctgattaaag acgaccagaa tcgcaatatg ttcgaacgcg gcagcgcgca
    18721 gattatgccg ttctttaaaa ctttgctggt ggagctcgcg ccagtgttcg actcgctcta
    18781 taacaaaatt attattaccg ggcataccga tgcgatggcc tacaaaaaca atatctacaa
    18841 caactggaac ctttcgggcg atcgcgcgct ttcagctcgt cgggtgctgg aagaggccgg
    18901 aatgccggaa gataaagtga tgcaggtaag cgcaatggcg gaccagatgc tgctggacgc
    18961 gaagaatccg caaagcgcgg gcaatcggcg tattgagatt atggtgctga ccaaaagtgc
    19021 gtccgatacg ctgtatcaat actttggtca gcatggggat aaagtggtgc agccgctggt
    19081 gcaaaagctg gataagcagc aggtgctttc gcagcgaatg cgctaaatgc tgaatcttta
    19141 cgcatttctc aaaacctgaa atcactgtat actttaccag tgttgagagg tgagcaatgc
    19201 gtaaaatcat tcatgtggat atggactgct ttttcgcagc ggtggagatg cgcgacaatc
    19261 ccgctctgcg cgatatccct attgctattg gcggcagccg cgaacgtcgg ggggtgatca
    19321 gcaccgccaa ttatcccgcg cgtaaatttg gcgtacgtag cgctatgcct actgggatgg
    19381 cgctcaaatt atgcccacat ctcaccttgc ttccggggcg ctttgacgcc tacaaagaag
    19441 cctcaaatca tatccgtgaa atcttctcgc gctacacctc gcgcattgaa ccgttgtcac
    19501 tggatgaggc ttatctcgat gtcaccgata gcgtccattg ccacggttct gcgaccctca
    19561 tcgcccagga aatccgccag acgatcttca acgagctgca actgacggca tctgcgggcg
    19621 tggcaccagt aaagtttctc gccaaaatcg cctccgacat gaataaaccc aacggtcagt
    19681 ttgtgattac gccggcagaa gttccggcat ttttacaaac cttaccactg gaaaaaatcc
    19741 ccggcgtcgg caaagtctcg gcggcaaaac tggaagcgat ggggctgcgg acctgcggtg
    19801 atgtacaaaa gtgtgatctg gtgattctgc ttaaacgctt tggcaaattt ggccgcattt
    19861 tgtgggagcg tagtcagggg attgacgagc gcgacgttaa cagcgaacgg ttacgaaaat
    19921 ccgtcggcgt ggaacgcacg atggcggaag atattcatca ctggtctgaa tgtgaagcga
    19981 ttatcgagcg gctgtatccg gaacttgaac gccgtctggc aaaggtaaaa cctgatttac
    20041 tgattgctcg ccagggggtg aaattaaagt tcgacgattt tcagcagacc acccaggagc
    20101 acgtctggcc gcggctgaat aaagctgact taatcgccac cgcgcgtaaa acctgggatg
    20161 aacgccgcgg cgggcgcggt gtgcgtctgg tggggctgca tgtgacgttg cttgacccgc
    20221 aaatggaaag acaactggtg ctgggattat gatgtatact attatgtata ttctggtgtg
    20281 cattattatg agggtatcac tgtatgcatc gaattctcgc tgaaaaatcg gtcaatatca
    20341 ctgagttacg taaaaaccca gctaaatact ttattgatca accagttgcg gttctttcta
    20401 ataatcgccc cgcaggatat ctcttaagtg ccagcgcatt cgaagcgtta atggacatgc
    20461 ttgctgaaca agaggagaaa aagcccataa aggcgcgctt tcgtccaagt gctgcaagat
    20521 tagaggaaat tacacgccgc gctaaacaat atcttaatga tatgacggat gatgatttca
    20581 atgactttaa ggaataagga tgcgggtatt caaaacaaaa cttattcgcc tgcaacttac
    20641 agcagaggaa cttgatgcgt taacggcgga ttttatttcc tataagcgtg acggtgtttt
    20701 gccagatata tttggtcgcg atgcactcta cgacgactcg tttacctggc cattaatcaa
    20761 atttgagcga gttgctcata ttcatctggc aaatgtgaat aatccatttc cgccacagtt
    20821 gcgccaattc agcagaacga atgacgaagc gcatttggta tattgtcagg gtgcgtttga
    20881 tgagcaagca tggttgctca ttgccattct gaagcctgaa cctcataaac tggctcgaga
    20941 taacaaccaa atgcataaaa ttggaaaaat ggcagaagcg tttcgcatgc gtttttgaat
    21001 ttatattatg aataacatac aaataagaga ttatcagcct ggcgattttc agcaactatg
    21061 cgctattttc attagagcgg ttacgatgac cgtcagtcag cattattcac tacaacaaat
    21121 tgccgcctgg gcgcagattg acgaatctcg ctggaaggag aaactcgcga aatcacaagt
    21181 gcgggttgca gttattaatg caaaactggt cggctttatt acctgcgttg aacactatat
    21241 cgatatgtta tttgttgacc ctgaatacac ccgccgtggg gttgccagcg ctttgttaaa
    21301 accttttatt aagtctgaat ccgaacttac ggtggacgca agcataaccg caaaaccctt
    21361 ttttgaacgt tatggttttc agacagttaa gcagcagcgc gttgaatgcc ggggagcgtg
    21421 gtttattaat ttttatatgc gttataaacc gcaacattaa atccagcttg caatgaaaat
    21481 aacgcccgcc tagtatgtgc gcgttttctt attccacaaa ctgcaaggag gtaaatcatg
    21541 acaaatcctt tatcaatgac tctttgcaga cctttccagg attaattctt tttttcttgc
    21601 cctggattcg tctgccattt cctgattttt taattgttac tctcaataat tcaagtcgca
    21661 gcacatgcaa cttgaagtat gacgagtcta tttacggaat ggtatatggg caattatatt
    21721 cgtcccttat ccgatgcggt atttaccatc gcatctgatg acctgtggat cgagagttta
    21781 gcgatccaac aattacacac cacggcaaat ttacccaaca tgcagcgcgt agtggggatg
    21841 ccagatttac accccggacg cggctacccg attggcgcag cgttcttctc cgcaggccgt
    21901 ttttacccag cactggtcgg caatgatatc ggctgtggca tggcgctatg gcaaacagat
    21961 attctcgctc gcaaatacaa cgccgataag tttgaaaagc gattatcagc tttggatgac
    22021 gttgctgaag aaagctggct ggaggaaaac ctgccgtcag catttgcaca gcatccgtgg
    22081 cgcagctcgc ttggctctat cggtggtggc aatcatttcg ctgaactgca acaagttgat
    22141 caaatcatca acgctgaact gtttgcactg gcaggtctgg atgcgcagca tctgcaactg
    22201 ctggttcata gcggctcgag ggggctaggg cagtctattt tacagcggca tattgcctcg
    22261 ttttcgcatc atggtttgcc tgaaggcagt gacgacgcgc tggcgtttgc gcgttttaat
    22321 cgccacctga tcgctttgcg cataatgcaa caggttaagg ccaccggaag tccggttctg
    22381 gatgtggcgc ataactttgt tagcgcgtgt cgaatcggtg atcaacaagg ggtcttacat
    22441 cgtaaaggtg ccacgcctga tgactgtggt ctggtggtta ttcccggttc acgcggtgat
    22501 tactccaggc tggttcagcc cgtcaggagt gaggaaacat tgcattcgct ggcgcatggg
    22561 gctgggcgta aatgggggcg caccgagtgt aaagggcgtc tggcagcgaa atacacagcg
    22621 acgcaactct cgcgtactga acttggcagt cgggtaattt gtcgcgataa acaactcatc
    22681 ttcgaagaag cgccacaagc ctataaatcg gctgaaagtg tggtgcaatg tcagggcagg
    22741 ccggattaat tattcctgtc gcgcggctgc gtccggtgct aacgctcaaa aacagtggag
    22801 ggaaaaaagg atgatcttgc tataactctc ctcagcgcag gggccggaag aatgttgtct
    22861 cgcagtgaaa aaggcgctgg acaggctaat taaagaagct gcccgacaag acgtcgcggt
    22921 aacggtgctg gaaacagaaa caggccgcta ctctgacacg ctgcgttcgg cgctgatttc
    22981 tctggatggc gacaacgcat gggcgttaag cgaaagctgg tgtggcacta ttcagtggat
    23041 ttgcccgagc ccgtatcgcc cacatcacgg gcgtaaaaac tggtttctgg gcattgggcg
    23101 ttttaccgct gatgagcagg agcaatccga tgcgatccgg tatgagacgc tgcgttcgtc
    23161 ggggccgggc ggtcaacatg tcaataaaac cgactcggcg gtacgcgcca cgcatttggc
    23221 atccggtatt agcgtgaagg ttcagtcaga gcgcagccag catgctaaca aacggctggc
    23281 gcgattgctg attgcctgga agctggatca gcagcaatag gaaaatagcg cggtgctgaa
    23341 atcacagcgg cgaatgttcc atcaccagat tgaacgtggc aacccgcgac gaacgtttac
    23401 agggatggct tttatcgaag gataatgaag gatgaaactg ccggaaggcg attaaacgcc
    23461 atccggcagc aaatcaaata attacttcgc cggaatttct ttcagcagtt cagtcagcag
    23521 tgtccagtaa tgacctacgc tttcgatgtg aacttgctca tccggagagt gtggaccggt
    23581 gatagttggc ccgatagaaa ccatgtccat ttccggatac ggttttttga acagaccaca
    23641 ttccaggccc gcgtggataa tctggatgtt cggcgtcttg ttgaacaggc gctgataggt
    23701 ttcacgtacc agatgcatca ccggagaatt agcgtccggc tgccagccag gatatgcgcc
    23761 tttcgcttcg gttttcgcgc cagccagttt acccagcgaa tccagcatgc tcaccacgta
    23821 gtctttaccg ctgtcgatta gtgaacggat caggcagtga atttctacgt tattgtcagt
    23881 catggtcacc acaccgacgt tcagggaggt ttcgaccaca ccttttgcca cgtcggaatt
    23941 gcggatcaca ccgttcgggg tggcgttcag cagacgaata aaggtatcgc gagatttcgc
    24001 aatcagggca gctttatcgt tcgctacaga gtccagcaac aaggccagat ttttctcttt
    24061 ctctgccagc tcgtttttca ggatctcctg ataggtattc accagagatt tcagtgcgtc
    24121 gactttatca gctgcgacag caatggtcgc aaaggcttca cgcgggatgg cgttacgcag
    24181 tgtgccgccg ttgaaatcga taaggcgcag gtccagttct tctgcatgac ccgccaggaa
    24241 gcgcaccagc agtttgttgg cattacccag gccaacgtgg atttcaccgc cggagtgacc
    24301 gcctttcaga ccttttaagg ttaacttgaa ggtttcaaaa ccagctggaa ccgcttcacg
    24361 atctaaatgc aggttggagg tgaagtcgat accccccgca caacccatgt agatttcacc
    24421 ttcttcttcg gagtcggtgt taatcagaat atcagcctgc aaccagttgc tctgtaagcc
    24481 gaacgcaccg tccataccgg cttcttcggt catggtcagc agcacttcca gcgggccgtg
    24541 aaccacgttt tcgtcagcca gaaccgccag tgcagaggcc ataccaatgc cgttatccgc
    24601 acccagcgtg gtgccgcgcg ctttaaccca ttcgccatca atataaggct ggataggatc
    24661 tttcgtgaag tcatgcacgg tgtcgttatt tttctgcggc accatatcga ggtgggcctg
    24721 taagacgacc ggtttacgat tttccatacc tgcggtagca ggttgacgaa tcaggatatt
    24781 acctacctga tcgcgttcga catggaaacc tttctctttt gcccaaccaa caatgtattc
    24841 agcgagttgc tcttcatgat aggacgggtg aggaatagaa cagattttgg caaaaatatc
    24901 ccacagcggc tgtggagata attgagacag ttcagacacg ttaagtctcc ttgtcgatca
    24961 cccgcaaaac agtattgcag gtcacagggt tagcagaaaa tgttgtcaac acaagacagg
    25021 cttgcgagat atgtattgag aataccactt tatcccgcgt cagggagagg cagtgcgtaa
    25081 aaagacgcgg actcatgtga aatactggtt tttagtgcgc cagatctcta taatctcgcg
    25141 caacctattt tcccctcgaa cactttttaa gccgtagata aacaggctgg gacacttcac
    25201 atgagcgaaa aatacatcgt cacctgggac atgttgcaga tccatgcacg taaactcgca
    25261 agccgactga tgccttctga acaatggaaa ggcattattg ccgtaagccg tggcggtctg
    25321 gtaccggggg cgttactggc gcgtgaactg ggtattcgtc atgtcgatac cgtttgtatt
    25381 tccagctacg atcacgacaa ccagcgcgag cttaaagtgc tgaaacgcgc agaaggcgat
    25441 ggcgaaggct tcatcgttat tgatgacctg gtggataccg gtggtactgc tgttgcgatt
    25501 cgtgaaatgt atccaaaagc gcactttgtc accatcttcg caaaaccggc tggtcgtccg
    25561 ctggttgatg actatgttgt tgatatcccg caagatacct ggatcgaaca gccgtgggat
    25621 atgggcgtcg tattcgtccc gccaatctcc ggtcgctaat cttttcaacg cctggcattg
    25681 ccgggcgttg ttctttttaa cttcaggcag gttacaatag tttccagtaa gtattctgga
    25741 ggctgcatcc atgacacagg caaacctgag cgaaaccctg ttcaaacccc gctttaaaca
    25801 tcctgaaacc tcgacgctag tccgccgctt taatcacggc gcacaaccgc ctgtgcagtc
    25861 ggctcttgat ggtaaaacca tccctcactg gtatcgcatg attaaccgtc tgatgtggat
    25921 ctggcgcggt attgacccac gtgaaatcct cgacgtccag gcacgtattg tgatgagcga
    25981 tgctgaacgt accgacgatg atttatacga taccgtgatt ggctaccgtg gtggcaactg
    26041 gatttatgag tgggccaccc aggcgatggt gtggcaacaa aaagcctgta cggaagaaga
    26101 tccgcaactc agtggtcgac actggctgca tgcggctacg ttgtacaaca ttgccgccta
    26161 tcctcatctg aaaggagatg acctggccga gcaagcgcag gctttatcaa accgcgccta
    26221 tgaagaggcc gcccagcgtc tgccgggcac gatgcggcag atggagttta ccgtacccgg
    26281 cggtgcgccc atcaccggct ttttgcatat gccgaaaggc gatggcccgt tcccgacagt
    26341 attaatgtgt ggtggtctgg atgcgatgca gacggactat tacagcctgt atgaacgtta
    26401 ttttgcgccg cgcggcattg cgatgctgac tattgatatg ccgtcggtgg gcttttcttc
    26461 aaaatggaag ctcacccagg actccagcct gttgcatcag cacgtcttaa aggcgctgcc
    26521 taacgtaccg tgggtggatc acactcgcgt cgcggccttt ggtttccgtt tcggcgctaa
    26581 cgttgccgtg cgtctggcat accttgaatc gccgcgtctg aaagcggttg cctgtcttgg
    26641 tccggtagtt catactctgt tgagtgattt taagtgccag caacaggtgc cggaaatgta
    26701 tcttgacgtt ctggcgagtc gtttggggat gcatgatgct tccgacgaag cgttacgcgt
    26761 ggagctgaat cgctattcat taaaagtgca aggattgctg gggcgtcgct gcccaacgcc
    26821 aatgttatca ggctactgga agaacgatcc gttcagcccg gaagaggact cacgcttaat
    26881 cacctcatca tctgctgacg gtaaattatt agagatccca tttaacccgg tgtatcggaa
    26941 ttttgacaaa ggtcttcagg aaatcaccgg ctggatcgaa aaacgcttgt gttaaaaatt
    27001 tgctaaattt tgccaatttg gtaaaacagt tgcatcacaa caggagatag caatgacgtt
    27061 accgagtgga cacccgaaga gcagattgat caaaaaattt accgcactag gcccgtatat
    27121 tcgtgaaggt aagtgcgaag ataatcgatt ctttttcgat tgtctggctg tatgcgtcaa
    27181 cgtgaaaccg gcaccggaag tgcgtgaatt ctggggctgg tggatggagc tggaagcgca
    27241 ggaatcccgt tttacctaca gctaccagtt tggtctgttc gataaagcag gcgactggaa
    27301 gagtgttccg gtaaaagata ctgaagtggt tgaacgactg gagcacaccc tgcgtgagtt
    27361 tcacgagaag ctgcgtgaac tgctgacgac gctgaatctg aagctggaac cggcgaatga
    27421 ttttcgtgat gaaccggtga agttaacggc gtgagtgaaa tgtgccggat gcatcacatc
    27481 cggcaatatt cattaaaact gatacgtcat cccaaccgcg acagtatcat cattattaat
    27541 attcagctta ttatcgctat ccagttggtt gattttataa tcaacaaacg ctgacatatt
    27601 tttgttgaaa taatacgtag caccgacgtc gatataattg accagatctt catcaccgat
    27661 gccctcaata tctttccctt tcgataagac ataacccagc gatggacgca gaccaaagtc
    27721 aaactggtat tgagcgaccg cttcaaagtt ctgtgtctta ttggcaaagc cgccagttat
    27781 tggcgtcatt ttgcgtgttt cagaatagaa agttgccaga taaatattat tggcatcgta
    27841 tttcagacct gttgcccatg cttctgcacg cttgcctgtg ccacggcttt gcaggttctg
    27901 ctcgttggtg cgatctgagt tggtataggc cccactaatg gcgaaatcgc tgccgccaaa
    27961 gtcatatgtc aatgacgtgc cgaagccatc gccgttttgc tttttaacgt cgcggttttc
    28021 gtttttccct tgatattgca gggttaagtt caggccatcg ataacgccga agaagtcggt
    28081 gttccgatac gtcgccagac cgctggcgcg tttggtcata aagttgtcgg tctgcgccga
    28141 ggaatcgcca ccaaattccg ggaacatatc ggtccaggct tccacgtcat acaacgcgcc
    28201 caggttacga ccataatcga aagaacccaa atctttatat ttcaacccgg caaaagcgag
    28261 acgcgttttt tgctgtgcag tatcactctc cgctttattt ccggcaaact ccgcttccca
    28321 ccgaccataa ccagtcagtt gatcgttaat ttgagtttca cctttaaaac caaaacggat
    28381 ataactctgg tcgccatctt tactggcgtt atcactcata taatgcatgg ctttaacttt
    28441 gccatagaca tccagtttat taccgtcttt attatatatt tctgcagcct gtacagatgc
    28501 agatgccaca atgcccatca ccactaatgc cagagtgctc tttttcattt tcattcctga
    28561 ttttaattaa cgcgcgaata ttcagcggga gagccccgtt gaaaacagga aagtttttaa
    28621 cctgagattg ttaaagatat attacagatt aatg
//
LOCUS       DAJTRC010000051        28420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.051, whole genome shotgun sequence.
ACCESSION   DAJTRC010000051 DAJTRC010000000
VERSION     DAJTRC010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.051"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            564..1334
                     /locus_tag="OO357_002317"
     CDS             564..1334
                     /locus_tag="OO357_002317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter ThrE family protein"
                     /protein_id="HCR5887239.1"
                     /translation="MQTEQQRAVTRLCIQCGLFLLQHGAESALVDELSSRLGRALGMD
                     SVESSISSNAIVLTTIKDGQCLTSTRKNHDRGINMHVVTEVQHIVILAEHHLLDYKGV
                     EKRFSQIQPLRYPRWLVALMVGLSCACFCKLNNGGWDGAVITFFASTTAMYIRQLLAQ
                     RHLHPQINFCLTAFAATTISGLLLQLPTFSNTPTIAMAASVLLLVPGFPLINAVADMF
                     KGHINTGLARWAIASLLTLATCVGVVMALTIWGLRGWV"
     gene            1325..1798
                     /locus_tag="OO357_002318"
     CDS             1325..1798
                     /locus_tag="OO357_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter"
                     /protein_id="HCR5887240.1"
                     /translation="MGVIEFLLALAQDMILAAIPAVGFAMVFNVPVRALRWCALLGSI
                     GHGSRMILMTSGLNIEWSTFMASMLVGTIGIQWSRWYLAHPKVFTVAAVIPMFPGISA
                     YTAMISSVKISQLGYSEPLMITLLTNFLTASSIVGALSIGLSIPGLWLYRKRPRV"
     gene            1905..2444
                     /gene="dnaT"
                     /locus_tag="OO357_002319"
     CDS             1905..2444
                     /gene="dnaT"
                     /locus_tag="OO357_002319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaT"
                     /protein_id="HCR5887241.1"
                     /translation="MSSRVLTPDVVGIDALVHDHQTVLAKAEGGVVAVFANNAPAFYA
                     VTPARLAELLALEEKLARPGSDVALDDQLYQEPQAAPVAVPMGKFAMYPDWQPDADFI
                     RLAALWGVALREPVTTEELASFIAYWQAEGKVFHHVQWQQKLARSLQIGRASNGGLPK
                     RDVNTVSEPDSQIPPGFRG"
     gene            2447..3184
                     /gene="dnaC"
                     /locus_tag="OO357_002320"
     CDS             2447..3184
                     /gene="dnaC"
                     /locus_tag="OO357_002320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication protein DnaC"
                     /protein_id="HCR5887242.1"
                     /translation="MKNVGDLMQRLQKMMPAHIKPAFKTGEELLAWQKEQGAIRSAAL
                     ERENRAMKMQRTFNRSGIRPLHQNCSFENYRVECEGQMNALSKARQYVEEFDGNIASF
                     IFSGKPGTGKNHLAAAICNELLLRGKSVLIITVADIMSAMKDTFRNSGTSEEQLLNDL
                     SNVDLLVIDEIGVQTESKYEKVIINQIVDRRSSSKRPTGMLTNSNMEEMTKLLGERVM
                     DRMRLGNSLWVIFNWDSYRSRVTGKEY"
     gene            3233..3727
                     /locus_tag="OO357_002321"
     CDS             3233..3727
                     /locus_tag="OO357_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2501 domain-containing protein"
                     /protein_id="HCR5887243.1"
                     /translation="MMKTIKHLLCCAIAASALISTGVHAASWKDALSSAASELGNQNS
                     TTQEGGWSLASLTNLLSSGNQALSADNMNNAAGILQYCAKQKLASVTDAENIKNQVLE
                     KLGLNSEEQKEDTNYLDGIQGLLKTKDGQQLNLDNIGTTPLAEKVKTKACDLVLKQGL
                     NFIS"
     gene            3967..6258
                     /gene="opgB"
                     /locus_tag="OO357_002322"
     CDS             3967..6258
                     /gene="opgB"
                     /locus_tag="OO357_002322"
                     /EC_number="2.7.8.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerol--membrane-oligosaccharide
                     glycerophosphotransferase"
                     /protein_id="HCR5887244.1"
                     /translation="MSELLSFALFLASVLIYAWKAGRNTWWFAATLTVLGLFVVLNIT
                     LFASDYFTGDGINDAVLYTLTNSLTGAGVSKYILPGIGIVLGLTAVFGALGWILRRRR
                     HHPHHFGYSLLALLLALGSVDASPAFRQITELVKSQSRDGDPDFAAYYKEPSKTIPDP
                     KLNLVYIYGESLERTYFDNEAFPDLTPELGALKNEGLDFSHTQQLPGTDYTIAGMVAS
                     QCGIPLFAPFEGNASASVSSFFPQNICLGDILKNSGYQNYFVQGANLRFAGKDVFLKS
                     HGFDHLYGSEELKSVVADPHYRNDWGFYDDTVLDEAWKKFEELSRSGQRFSLFTLTVD
                     THHPDGFISRTCNRKKYDFDGKPNQSFSAVSCSQENIATFINKIKASPWFKDTVIVVS
                     SDHLAMNNTAWKYLNKQDRNNLFFVIRGDKPQQKTLAVKRNTMDNGATVLDILGGDNY
                     LGLGRSSLSGQSMSEIFLNIKEKTLAWKPDIIRLWKFPKEMKEFTIDQQKNMIAFSGS
                     HFRLPLLLRVSDKRVEPLPESEYSAPLRFQLADFAPRDNFVWVDSCYKMAQLWAPELA
                     LSTDWCVSQGQLGGQQIVQHVDKTTWKSKTAFKDTVIDMARYKGNVDTLKIVDNDIRY
                     KADSFIFNVAGAPEEVKQFSGISRPESWGRWSNAQLGDEVKIEYKHPLPKKFDLVITA
                     KAYGNNASRPIPVRVGNEEQTLVLGNEVTTTTLHFDNPTDADTLVIVPPEPVSTNEGN
                     ILGHSPRKLGIGMVEIKVVEREG"
     gene            complement(6396..7418)
                     /gene="lgoD"
                     /locus_tag="OO357_002323"
                     /pseudo
     CDS             complement(6396..7418)
                     /gene="lgoD"
                     /locus_tag="OO357_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418778.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-galactonate oxidoreductase"
     gene            7557..8471
                     /locus_tag="OO357_002324"
     CDS             7557..8471
                     /locus_tag="OO357_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5887245.1"
                     /translation="MSRSQNLRHNVINQVIDDMARGHIPSPLPSQSALAEMYNISRTT
                     VRHILSQLRECGVLTQVGNDYVIARKPDHDDGFACTTASMSEQNKVFEQAFFTMINQR
                     QLRPRETFSELQLARAAGVSPVVVREYLLKFGRYNLIQSEKRGQWSMKQFDQSYAEQL
                     FELREMLETHSLQHFLNLPDHDPRWLQAKTMLERHRLLRDNIGNSFRMFSQLDRDFHS
                     LLLSAADNIFFDQSLEIISVIFHFHYQWDESDLKQRNIIAVDEHMTILSALICRSDLD
                     ATLALRNHLNSAKQSMIRSINENTRYAH"
     gene            8686..10047
                     /locus_tag="OO357_002325"
     CDS             8686..10047
                     /locus_tag="OO357_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5887246.1"
                     /translation="MEKENITLDPRSSFTPSSSADIPVPPDGLVQRSTRIKRIQTTAM
                     LLLFFAAVINYLDRSSLSVANLTIREELGLSATEIGALLSVFSLAYGIAQLPCGPLLD
                     RKGPRLMLGLGMFFWSLFQAMSGMVHSFTQFVLVRIGMGIGEAPMNPCGVKVINDWFN
                     IKERGRPMGFFNAASTIGVAVSPPILAAMMLVMGWRGMFITIGVLGIFLAIGWYMLYR
                     NREHVELTAVEQAYLNAGSVNARRDPLNFAEWRSLFRNRTMWGMMLGFSGINYTAWLY
                     LAWLPGYLQTAYNLDLKSTGLMAAIPFLFGAAGMLVNGYVTDWLVKGGMAPIKSRKIC
                     IITGMFCSAAFTLIVPQATTSMMAVLLIGMALFCIHFAGTSCWGLIHVAVASRMTASV
                     GSIQNFASFICASFAPIITGFIVDTTHSFRLALIICGCVTAAGALAYIFLVRQPINDP
                     RKD"
     gene            complement(10096..11760)
                     /gene="tsr"
                     /locus_tag="OO357_002326"
     CDS             complement(10096..11760)
                     /gene="tsr"
                     /locus_tag="OO357_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HCR5887247.1"
                     /translation="MLKRIKIVTSLLLVLAVFGLLQLTSGGLFFNALKNDKENFTVLQ
                     TIRQQQPTLNGSWVALLQTRNTLNRAGIRYMMDQNNIGSGSTVAELMQSASISLKQAE
                     KNWADYEALPRDPRQSTAAAAEIKRNYDIYHNALAELIQLLGAGKINEFFDQPTQGYQ
                     DGFEKQYVAYMEQNDRLYDIAVSDNNASYSQAMWILVGVMIVVLAVIFAVWFGIKASL
                     VAPMNRLIDSIRHIAGGDLVKPIEVDGSNEMGQLAESLRHMQGELMRTVGDVRNGANA
                     IYSGASEIATGNNDLSSRTEQQAASLEETAASMEQLTATVKQNAENARQASHLALSAS
                     ETAQRGGKVVDNVVQTMRDISTSSQKIADIISVIDGITFQTNILALNAAVEAARAGEQ
                     GRGFAVVAGEVRNLAQRSAQAVREIKSLIEDSVGKVDVGSTLVESAGETMAEIVSAVT
                     RVTDIMGEISSASDEQSRGIDQVGLAVAEMDRVTQQNAALVEESAAAAAALEEQASRL
                     TEAVAVFRIQQQQQQQRETSAVVKTVTPATPRKMAVADSGENWETF"
     gene            complement(11879..12325)
                     /gene="hpaR"
                     /locus_tag="OO357_002327"
     CDS             complement(11879..12325)
                     /gene="hpaR"
                     /locus_tag="OO357_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HCR5887248.1"
                     /translation="MHDSLTIALLQAREAAMSYFRPIVKRHNLTEQQWRIVRILAESP
                     SMDFHDLAYRACILRPSLTGILTRMERDGLVLRLKPINDQRKLYISLTKEGQALYNRA
                     QTQIEEAYRQIEAQFTAEKMQQLTHLLEEFIALGNSRQEDIPGDNE"
     gene            12597..13886
                     /locus_tag="OO357_002328"
     CDS             12597..13886
                     /locus_tag="OO357_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710093.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate degradation bifunctional
                     isomerase/decarboxylase"
                     /protein_id="HCR5887249.1"
                     /translation="MKGTIFAVALNHRSQLDAWQEAFQQSPYKAPPKTAVWFIKPRNT
                     VIGCGEPIPFPQGEKVLSGATVALIVGKTATKVREEDAAEYIAGYALDNDVSLPEESF
                     YRPAIKAKCRDGFCPIGETVALSNVDNLTIYTEINGRPADHWNTADLQRNAAQLLSAL
                     SEFATLNPGDAILLGTPQARVEIQPSDRVRVLAEGFPPLENPVVDEREVTTRKSFPTL
                     PHPHGTLFALGLNYADHASELEFKPPEEPLVFLKAPNTLTGDNQTSVRPNNIEYMHYE
                     AELVVVIGKQARNVSEADAMDYVAGYTVCNDYAIRDYLENYYRPNLRVKSRDGLTPML
                     STIVPKEAIPDPHNLTLRTFVNGELRQQGTTADLIFSVPFLIAYLSEFMTLNPGDMIA
                     TGTPKGLSDVVPGDEVVVEVEGVGRLVNRIVSEETAK"
     gene            13883..15349
                     /gene="hpaE"
                     /locus_tag="OO357_002329"
     CDS             13883..15349
                     /gene="hpaE"
                     /locus_tag="OO357_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HCR5887250.1"
                     /translation="MKKVNHWINGKNVAGNDYFQTTNPATGEVLADVASGGEAEINQA
                     VAAAKEAFPKWADLPMKERARLMRRLGDLIDQNVPEIAAMETADTGLPIHQTKNVLIP
                     RASHNFEFFAEVCQQMNGKTYPVDDKMLNYTLVQPVGVCALASPWNVPFMTATWKVAP
                     CLALGNTAVLKMSELSPLTADRLGELALEAGIPAGVLNVVQGYGATAGDALVRHHDVR
                     AVSFTGGTATGRNIMKNAGLKKYSMELGGKSPVLIFEDADIERALDAALFTIFSINGE
                     RCTAGSRIFIQQSIYPEFVKRFAERANRLRVGDPNDPNTQVRALISQQHWEKVSGYIR
                     LGIEEGATLLAGGPDKPSDLPAHLKGGNFLRPTVLADVDNRMRVAQEEIFGPVACLLP
                     FKDEAEGLRLANDVEYGLASYIWTQDVSKVLRLARGIEAGMVFVNTQNVRDLRQPFGG
                     VKASGTGREGGEYSFEVFAEMKNVCISMGDHPIPKWGV"
     gene            15351..16202
                     /gene="hpaD"
                     /locus_tag="OO357_002330"
     CDS             15351..16202
                     /gene="hpaD"
                     /locus_tag="OO357_002330"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HCR5887251.1"
                     /translation="MGKLALAAKITHVPSMYLSELPGKNHGCRQGAIDGHKEISKRCR
                     EMGVDTIIVFDTHWLVNSAYHINCADHFEGVYTSNELPHFIRDMTYNYEGNPELGQLI
                     AEEALKLGVRAKAHNIPSLKLEYGTLVPMRYMNEDKHFKVVSISAFCTVHDFADSRKL
                     GEAILKAIEQYDGTVAVLASGSLSHRFIDDQRAEEGMNSYTREFDRQMDERVVKLWRE
                     GQFKEFCNMLPEYADYCYGEGNMHDTVMLLGMLGWDKYDGKVEFITELFPSSGTGQVN
                     AVFPLPA"
     gene            16212..16592
                     /locus_tag="OO357_002331"
     CDS             16212..16592
                     /locus_tag="OO357_002331"
                     /EC_number="5.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate
                     Delta-isomerase"
                     /protein_id="HCR5887252.1"
                     /translation="MPHFIVECTENIREEARLPELFASVNTALAATGIFPLGGIRSRA
                     HWIDTWQMADGKHDYAFVHMTLKIGSGRSLESRQEVGEMLFDLIKTHFASLMESRYLA
                     LSFEIAELHPTLNFKQNNVHALFK"
     gene            16660..17463
                     /gene="hpaH"
                     /locus_tag="OO357_002332"
     CDS             16660..17463
                     /gene="hpaH"
                     /locus_tag="OO357_002332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710089.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HCR5887253.1"
                     /translation="MFDKHTHTLIAQRLDQAEKQREQIRAISLDYPEITIEDAYAVQR
                     EWVRLKIAESRTLKGHKIGLTSKAMQASSQISEPDYGALLDDMFFHDGSDIPTDRFIV
                     PRIEVELAFVLAKPLRGPNCTLFDVYNATDYVIPALELIDARCHNIDPETQRPRKVFD
                     TISDNAANAGVILGGRPIKPDELDLRWISALMYRNGVIEETGVAAGVLNHPANGVAWL
                     ANKLAPYDVQLETGQIILGGSFTLPVPARKGDTFHVDYGNMGSISCRFV"
     gene            17474..18277
                     /gene="hpaI"
                     /locus_tag="OO357_002333"
     CDS             17474..18277
                     /gene="hpaI"
                     /locus_tag="OO357_002333"
                     /EC_number="4.1.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-2-oxoheptanedioate aldolase"
                     /protein_id="HCR5887254.1"
                     /translation="MENSFKAALKAGRPQIGLWLGLSSSYSAELLAGPGFDWLLIDGE
                     HAPNNVQTVLTQLQAIAPYPSQPVVRPSWNDPVQIKQLLDVGTQTLLVPMVQNADEAR
                     KAVRATRYPPAGIRGVGSALARASRWNRIPDYLQKANDQMCVLVQIETREAMKNLPQI
                     LDVEGVDGVFIGPADLSADMGYAGNPQHPEVQAAIEQAIVQIREAGKAPGILIANEQL
                     AKRYLELGALFVAVGVDTTLLARAAEALAARFGNSSSISMAKQNNNSVY"
     gene            18299..19654
                     /gene="hpaX"
                     /locus_tag="OO357_002334"
     CDS             18299..19654
                     /gene="hpaX"
                     /locus_tag="OO357_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710087.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate permease"
                     /protein_id="HCR5887255.1"
                     /translation="MTTSTLQYKDNKTVEIEHRVINKLFRRLIIFLFILFVFSFLDRI
                     NIGFAGLTMGKDLGLTSTMFGLAATLFYVTYVMCGIPSNMMLAKIGARRWIAGIMVVW
                     GIASTCTMFATSPETLYLLRMLVGIAEAGFLPGILVYLTWWFPAYHRARANALFMIAM
                     PVTMMLGLILSGYILALDGLWNLKGWQWLFLLEGLPSVILGVVTWFYLNDTPDQATWL
                     DENEKQALKTMILREQESAFAHASVPRSTLREVLTPAIMLYTLAYFCPTNTLSAINIW
                     TPQILQSFNTGSSNIMIGLLAAIPQFCTIFGMIWWSRRSDRLKERKKHTILPYLFAAA
                     GWLLASATDHSLIQLSSIIMASMGSFTAMTIFWTTPDQVISLQSRAVAMAVINAVGNV
                     GSAVSPLLIGILKDATGSFSSGLWFVAGLLVLGALVMTRIPMGSREHSLSSELIAPKP
                     Q"
     gene            19664..20554
                     /gene="hpaA"
                     /locus_tag="OO357_002335"
     CDS             19664..20554
                     /gene="hpaA"
                     /locus_tag="OO357_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate catabolism regulatory
                     protein HpaA"
                     /protein_id="HCR5887256.1"
                     /translation="MSSEAIFEHGYENIDLVREYDARYAGEDVHYETFARLANFFGRD
                     MRPHWHDRYFQLHYLVTGRITLQLDEHFYSVRAPLFVLTPPSVPHTFFTDPDSDGHVL
                     TVRQELIWPLVEKLWPGSGDAAMLQGICLSLEGMEHTLAALNHYWPIISLEFQQNKQG
                     REILLQSLAQSIFTILLREAPPNDISTCSVRGEMCLFQKFNRLIDENYRQHLMVPEYA
                     TMLGMSESRLTELCRRFANQSPKRLIFERVSREARRLLLYSEQSINQIALDLGYKDPA
                     YFARFFNRMMGCSPTQFRGR"
     gene            20581..21518
                     /locus_tag="OO357_002336"
                     /pseudo
     CDS             20581..21518
                     /locus_tag="OO357_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601398.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
     gene            21745..23303
                     /gene="hpaB"
                     /locus_tag="OO357_002337"
                     /pseudo
     CDS             21745..23303
                     /gene="hpaB"
                     /locus_tag="OO357_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000801462.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase,
                     oxygenase component"
     gene            23321..23833
                     /locus_tag="OO357_002338"
     CDS             23321..23833
                     /locus_tag="OO357_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyphenylacetate 3-monooxygenase reductase
                     subunit"
                     /protein_id="HCR5887257.1"
                     /translation="MQLDEQRLRFHDAMASLSAAVNIITTEGDAGQCGITATAVCSVT
                     DTPPSLMVCINANSAMNPVFQGNGKLCVNVLNHEQELMARHFAGMTGMAMEERFSLSC
                     WQKGPLAQPVLKGSLASLEGEIRDVQAIGTHLVYLVEIKNIILSAEGHGLIYFKRRFH
                     PVMLEMEAAI"
     gene            24226..26376
                     /gene="btsT"
                     /locus_tag="OO357_002339"
     CDS             24226..26376
                     /gene="btsT"
                     /locus_tag="OO357_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313340.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate/proton symporter BtsT"
                     /protein_id="HCR5887258.1"
                     /translation="MDTKKLFKHIPWVILGIIGAFCLAVVALRRGEHVSALWIVVASV
                     SVYLVAYRYYSLYIAQKVMKLDPTRATPAVINNDGLNYVPTNRYVLFGHHFAAIAGAG
                     PLVGPVLAAQMGYLPGTLWLLAGVVLAGAVQDFMVLFISSRRNGASLGEMIKEEMGPV
                     PGTIALFGCFLIMIIILAVLALIVVKALAESPWGVFTVCSTVPIALFMGIYMRFIRPG
                     RVGEVSVIGIVLLVASIYFGGVIAHDPYWGPALTFKDTTITFALIGYAFVSALLPVWL
                     ILAPRDYLATFLKIGVIVGLALGIVVLNPELKMPAMTQYIDGTGPLWKGALFPFLFIT
                     IACGAVSGFHALISSGTTPKLLANETDARFIGYGAMLMESFVAIMALVAASIIEPGLY
                     FAMNTPPAGLGITMPNLHEMGGENAPIIMAQLKDVTAHAAATVSSWGFVISPEQILQT
                     AKDIGEPSVLNRAGGAPTLAVGIAHVFHKVLPMADMGFWYHFGILFEALFILTALDAG
                     TRSGRFMLQDLLGNFIPFLKKTDSLVAGIIGTAGCVGLWGYLLYQGVVDPLGGVKSLW
                     PLFGISNQMLAAVALVLGTVVLIKMKRTQYIWVTVVPAVWLLICTTWALGLKLFSTNP
                     QMEGFFYMASQYKEKIANGTDLTAQQIANMNHIVVNNYTNAGLSILFLIVVYSIIFYG
                     FKTWLAVRNSDKRTDKETPYVPIPEGGVKISSHH"
     gene            26426..26629
                     /locus_tag="OO357_002340"
     CDS             26426..26629
                     /locus_tag="OO357_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5887259.1"
                     /translation="MFGNLGQAKKYLGQAAKMLIGIPDYDNYVEHMKTNHPDKPYMSY
                     EEFFRERQNARYGGDGKGGMRCC"
     gene            26640..27596
                     /gene="yjiA"
                     /locus_tag="OO357_002341"
     CDS             26640..27596
                     /gene="yjiA"
                     /locus_tag="OO357_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HCR5887260.1"
                     /translation="MNPIAVTLLTGFLGAGKTTLLRHILNEQHGYKIAVIENEFGEVS
                     VDDQLIGDRATQIKTLTNGCICCSRSNELEDALLDLLDNLDKGNIQFDRLVIECTGMA
                     DPGPIIQTFFSHEILCQLYLLDGVIALVDAVHADEQMNQFTIAQSQVGYADRILLTKT
                     DVAGEAEKLRERLARINARAPVYTVTHGDIDLGLLFNTNGFMLEENVVSTKPRFHFIA
                     DKQNDISSIVVELDYPVDISEVSRVMENLLLESADKLLRYKGMLWIDGEPNRLLFQGV
                     QRLYSADWDRPWGDEKPHSTMVFIGIQLPEEEIRAAFAGLRK"
     gene            complement(27665..>28420)
                     /locus_tag="OO357_002342"
     CDS             complement(27665..>28420)
                     /locus_tag="OO357_002342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HCR5887261.1"
                     /translation="YTKILENDFEHPSIRRKIASLSNLGVDEWIPPVMAFMNRMARTE
                     DFNLDDFSQFITAFEKVYMHGWLKKQIKSQREMVCYSALVAINNDMPFDSVINQINQH
                     ADNSGFIAALDEDLYEPRPNQVNLIKAILLRLDMEQQDESVIKTYTGRITIEHILPQA
                     LVNEYWINRFQPQEHVYWLHKIGNLTLISGSKNSETQHYDFIKKKSIYEKLNSKSSFY
                     LTKDVCNSSEWGLAELKMRHEKMKTQLKKLWLV"
BASE COUNT         6956 a         7426 c         7126 g         6912 t
ORIGIN      
        1 cttaaatgtt tccagcaaat taatagatta agcgaataaa ttatccttag cattcacttt
       61 catatcagca tactcctgct gcacaacaac tccactgcat gtgtcagaag gttagcatca
      121 aatgataacc tggtgaaata gcctggagac atttaaaata tctacattaa gttgttagaa
      181 atacttaatg aaaaacagaa atctcattca tttaatttat aattaaatga tatctcttcc
      241 cgcagaatgc gggaatcaca caatcaatat ttattcaata aaatctgatg attttaactt
      301 gttaacacta aaaaacgcgc ccagctatct attatcctga cccgtaaagg ctaaacaata
      361 aattcattat agtgataata ttcatgtgat agtttgtttg cggcgagaga taattcgctt
      421 tttatcaccg agcttgtccc aataaccagg attaatcagc gcaattttga tcttaaccta
      481 ccgcaaaaca accgtgcttc atgatacaaa tggattctca ttatctgaac aatacgcagc
      541 acgtctatga caaagggcga gttatgcaaa ctgagcaaca gcgagccgta acgcggcttt
      601 gtatccagtg tggattattt cttttgcaac atggtgcgga aagcgcgttg gttgatgagc
      661 tttcctcacg actgggtcgg gcgctgggaa tggacagcgt cgaaagttct atctcttcga
      721 acgccatagt gctgacaact attaaagatg ggcaatgcct gacatcgaca cgtaaaaatc
      781 acgatcgcgg cattaatatg catgtggtga ctgaagtcca gcacattgtg attcttgcgg
      841 agcatcatct gctggattac aaaggcgtag agaaacgatt tagccaaatt caaccattac
      901 gttacccaag atggctggta gccttaatgg ttggcctttc ttgcgcctgt ttctgtaaac
      961 tcaataacgg tggctgggat ggtgccgtca tcaccttctt tgccagtacg accgcgatgt
     1021 atatccgcca gctgctggca caacgtcatc ttcatccaca gatcaacttt tgccttaccg
     1081 ctttcgccgc caccaccatt tccggattgc ttttgcaact ccccactttc agcaataccc
     1141 ccaccattgc gatggccgcc agcgttctgc tgctagtgcc gggctttccg ttgattaatg
     1201 ccgtcgccga tatgtttaaa ggccacatca ataccggact ggcacgctgg gcgatcgcca
     1261 gtctgctgac actggctacc tgcgtcggcg tagtgatggc actgacgatt tgggggctac
     1321 gcggatgggt gtgatcgaat ttctgttagc gttggcgcag gatatgatcc tcgccgccat
     1381 tcctgcggtc ggctttgcga tggtgttcaa cgttcccgtg cgggcgttac gctggtgtgc
     1441 gctgcttggc tcgataggtc atggttcccg aatgatcttg atgaccagcg ggttgaatat
     1501 tgagtggtca acctttatgg cttctatgct ggtcggtacc attggtattc aatggtcgcg
     1561 ctggtatctg gcgcatccga aagtgtttac cgtggcggcc gttatcccta tgttcccggg
     1621 catatcggct tataccgcaa tgatttcgtc ggtaaaaatc agccagttag gttacagcga
     1681 accgttgatg attaccctgt taaccaactt tcttacagct tcatcgattg ttggtgcgtt
     1741 atccatcggt ctttccattc ctggattatg gttgtaccgc aagcgccctc gcgtataaaa
     1801 ttgcctcgct taagcacacg gatgagagac agcctcctct cctccgtgtg ttactataaa
     1861 agttatctcc cttctcgttc atcgttccat atttgagaaa cagtatgtct tccagagttt
     1921 tgaccccgga cgtcgttggt attgacgccc tggtacacga tcaccaaacc gttctggcaa
     1981 aagctgaagg cggtgtggtt gccgtatttg ctaacaatgc cccggcgttt tatgccgtca
     2041 cgcctgcacg cctggctgaa ctgctggcgc tggaagaaaa gctagcgcgt ccgggaagcg
     2101 atgtcgctct ggacgatcaa ctctatcagg aaccgcaagc cgctcccgtt gctgtaccca
     2161 tggggaaatt cgccatgtat ccggactggc aacccgatgc cgattttatc cgcctggcgg
     2221 cgctatgggg cgtggcgcta agagagccgg tgaccaccga agaactggcc tcattcattg
     2281 cctactggca ggcggaaggt aaagtctttc accatgtgca gtggcaacaa aaactggcgc
     2341 gcagcctgca aatcggtcgt gccagcaacg gcggactgcc gaaacgagat gtgaatacgg
     2401 tcagtgaacc tgacagccaa attccaccag gattcagagg gtaacgatga aaaacgttgg
     2461 cgacctgatg caacgcctgc aaaaaatgat gcctgcccat atcaaacctg cattcaaaac
     2521 gggtgaagag ctactggcct ggcagaaaga acaaggggcg atccgctccg ccgctctcga
     2581 acgtgaaaat cgggcgatga aaatgcagcg cacctttaac cgctccggta ttcgtccact
     2641 gcatcagaac tgctcctttg agaactatcg cgttgagtgt gaagggcaga tgaatgcgtt
     2701 aagcaaagcg cgccagtatg tcgaagagtt tgacggcaac atcgccagct tcatcttttc
     2761 tggtaagcca ggaaccggca aaaaccatct ggcggcggca atctgcaacg agctgctgct
     2821 acgcggtaaa tccgtattga tcatcaccgt ggccgatatt atgtcggcga tgaaagatac
     2881 cttcaggaat agcggtacca gcgaagaaca actgcttaac gatctgagca acgttgatct
     2941 gctggtgatc gatgagatcg gcgtgcagac cgaatcgaaa tacgaaaaag tgatcatcaa
     3001 ccagatcgtc gatcgccgct cttcttccaa acgcccaacc gggatgctga ccaacagtaa
     3061 tatggaagag atgaccaagt tgctgggcga acgcgtgatg gaccgtatgc gcctgggtaa
     3121 cagtttatgg gtgatcttca actgggatag ctaccgtagc cgggtaacgg gtaaagagta
     3181 ttaatccccg tttctggcgg tatactgcga cacaattcca ccttgagtaa tgatgatgaa
     3241 aactattaaa catcttctgt gctgtgccat tgccgccagc gcattaattt ccaccggggt
     3301 gcatgctgcg tcctggaaag atgcgctctc cagcgccgcc agcgaacttg gcaaccaaaa
     3361 cagcacgaca caggaaggcg gttggtcgct cgcgtcatta actaacttgc ttagcagcgg
     3421 aaaccaagcc ttaagcgcag ataacatgaa caatgccgca ggcattctgc aatactgcgc
     3481 gaagcaaaag ctggcttcgg taaccgatgc cgaaaacatc aagaaccagg tgctggaaaa
     3541 gctgggcctg aacagtgaag agcaaaaaga agacaccaac tatctggacg gtattcaggg
     3601 tttgctgaaa acaaaagatg gtcagcaact caatctggat aacatcggaa cgactccgct
     3661 ggcagaaaag gtgaaaacca aagcctgcga tctggtgtta aagcaggggc tgaacttcat
     3721 ttcctgaacc gcgaaatgtt acccggctga cgcggctctc tcctgccctt ttccctgctc
     3781 ctggacggtt ttccccctgt tacacgggaa gcgaccgcga tcaaaaaagt ctgtttataa
     3841 actaattcct aacaacatcg catttttttg acactagaat tgcagcaata tggcgacgcc
     3901 attacattgt gcttctcaaa aagcttaaac gttggctaaa cggcccggtt tagtgaggat
     3961 cgcccattgt cagaactact ctctttcgcc ctttttctcg cctctgtgct gatttacgca
     4021 tggaaagcgg gacgtaacac ctggtggttt gcagccacgt taacggtgct ggggctattt
     4081 gtcgttttaa atatcaccct gtttgccagc gactatttta ctggcgatgg tattaacgac
     4141 gcggttctct ataccttaac caacagcctg accggtgctg gcgtcagcaa atacatcctg
     4201 ccgggtatcg gcattgtgct ggggctgacg gcggtgttcg gtgcgctggg ctggatcctg
     4261 cgccgtcgtc gtcatcatcc gcaccatttt ggttacagcc tgctggcgct cttactggcg
     4321 ctgggttcgg tggacgccag cccggcattt cgtcagataa cggaactggt gaaatcccag
     4381 tcacgcgatg gcgacccgga ctttgcggct tattataaag agccgtcgaa aactatccct
     4441 gacccgaaac tcaacctggt ttatatctac ggcgaaagtc tcgagcggac ctattttgat
     4501 aacgaagctt tcccggatct cacgcctgaa ctgggcgcgt tgaaaaatga aggcctggat
     4561 ttcagccaca cacagcagct gccaggaacg gattacacga ttgcgggcat ggtggcttct
     4621 cagtgcggca taccgctgtt cgcccccttt gaaggcaacg cctccgcctc tgtctccagc
     4681 ttcttcccgc agaacatctg tctgggcgat atcctgaaaa actcgggtta tcagaactat
     4741 ttcgtgcagg gcgcaaatct gcgttttgcc ggtaaagatg tgttcctgaa atcgcacggc
     4801 ttcgaccact tatacggctc agaagagctg aaaagcgtgg tagccgaccc gcactatcgc
     4861 aacgactggg gattctacga cgataccgtt ctcgatgaag cgtggaaaaa gtttgaagag
     4921 ctttcccgct caggtcagcg attctcactg tttaccctga cagtcgatac ccatcacccg
     4981 gatggtttta tctcgcgcac ctgtaaccgc aaaaaatatg attttgacgg taagccgaat
     5041 cagtcattca gcgcggtaag ttgcagccag gagaatatcg cgacgtttat caacaaaatc
     5101 aaagcgtcac cgtggtttaa agatactgtc atcgtcgtct cttctgacca tttagcgatg
     5161 aacaacacgg cgtggaaata cctcaataag caggatcgca ataacctgtt ttttgtcatt
     5221 cgtggcgaca agccgcagca aaagacgctg gcagtgaagc gtaacacgat ggataacggc
     5281 gcgacggtgc tggacattct cggtggcgat aactatctcg gacttggtcg tagcagttta
     5341 tccgggcagt cgatgtcgga aatcttcctc aatatcaaag agaaaacatt ggcgtggaag
     5401 ccagatatca tccgcctgtg gaaattccct aaagagatga aagagttcac catcgaccag
     5461 cagaaaaaca tgattgcctt ctcgggtagc catttccgtt tgccgctgct gttgcgggtt
     5521 tcagacaaac gcgtggaacc gctgccggaa agcgaatact cagcaccgct gcgtttccag
     5581 ctggccgatt tcgctccacg cgacaatttc gtctgggttg acagttgcta caagatggca
     5641 caactctggg ctccggaact ggcactttcc accgactggt gtgtctcgca ggggcagctt
     5701 ggcggtcagc aaattgttca gcatgttgac aaaacaacat ggaagagcaa gacggcattt
     5761 aaagatacgg tcatcgacat ggcgcgttac aaaggcaatg tcgatacgct gaagattgtt
     5821 gataacgata ttcgctacaa agccgacagt ttcatcttta acgtcgccgg tgcgccagaa
     5881 gaggtgaaac agtttagcgg gatttcccgt ccggagtcgt ggggccgctg gtccaacgcg
     5941 cagctgggcg atgaagtaaa aatcgagtac aagcatccgc tgccgaagaa atttgacctg
     6001 gtgattaccg ccaaagcata cggcaataac gccagccgtc ctattccggt acgcgtaggc
     6061 aatgaagaac aaactcttgt gctgggcaat gaagtgacca ccaccacact gcatttcgat
     6121 aacccaaccg atgccgacac actggtaatt gtgccgccgg aacctgtctc aaccaacgaa
     6181 gggaatatcc tcggacactc gccgcgtaag ctcgggatcg gcatggtgga aattaaagtg
     6241 gtagaacgtg aagggtaatt cctgactgat gtgtaggtcg catagggtgc gacctacacg
     6301 ccttctccgg cttacaacac attattaaga aacaaaaaaa acagcgccca acacttcccc
     6361 ggacgctgca tggcaacttt aataatctcg ccttatcaga aagtaattac acccttaatc
     6421 aactcacgat tgttaatcac atcgcgctcg taggtttctg ccagcgtggc gaacggatag
     6481 cgatgggtta acatcatgtc ggcagtgatt ttcccttccg ccatcagtcg accgacttta
     6541 gcgaaatctt ccggcgtggc gttgcggctg cccatcatcg tcgtttcttt tttatggaat
     6601 tccggatcgg agaactgcaa ctcaccttta aacagaccga caaataccac cgtgccgccg
     6661 tgacgaatca gattcacggt attattcatc gcatgttgat tacctgtcgc gtcgatcact
     6721 ttctgcgcca gcgaaccacc aaactgcgcc cgcagctgcg cgtcaaaatc ttcggctgac
     6781 gggtccagca cgggcaactc cagacgcgtt gccacatgtt cacggcgcgc cggactggta
     6841 tccgccacca ccacctgtgc gccatcggct ttagcgattg ccgccgcgcc cagaccgatt
     6901 ggccccgccc cgaccaccag cacctgctcg ccgggagcaa tggctgcgcg acgcaccgca
     6961 tgagcgctaa tagcgaaagg ttcaatcaat gcggccgcct gcgggtcaat accgtctgcg
     7021 ggcaaaatgt tcgccaccgg cacgctcaaa tactcactaa aaccgccatc ctgatgcacg
     7081 ccaatgactg aaattttttc acagcagttg gtacgcccgc ttttacacgc cgggcattgc
     7141 tgacaggcaa cataagggat cacagcaact tgctgaccat ttttaagatc agcaatattt
     7201 ttacccagcc caacaatctc cccacatatt tcatggccta aaacccgtgg ataactaaaa
     7261 aatggttgat ttccacccta ggcatgaata tcggtaccgc aaatcccgac agacttaatt
     7321 tttattaatg cttcattgtc acccggaata ggtatctcgc gttgtttcca gactaattct
     7381 ttcggctgct ggcaaattaa aacattcatc gtagacatga ttcggctatc cattttttgt
     7441 tgccttagat atcaatgaaa atgaaaaatg gcagcggaac ggaaaatctg ttttgtgaaa
     7501 acacacgcat aaaaatgttt taaatcgggt tttaatggac tcacaaggag gaaaatatga
     7561 gtcgttcaca aaatttacgc cacaatgtga ttaaccaggt cattgatgat atggcgcggg
     7621 gtcatatccc ttccccactt ccatcgcaaa gtgcgctggc ggaaatgtac aacatcagcc
     7681 gcactacagt gcgtcatatt ctcagccaat tacgcgaatg cggcgtctta acgcaggttg
     7741 gaaacgacta tgttattgcg cgtaaacctg accatgatga cggtttcgcc tgtactaccg
     7801 cttcaatgag tgaacaaaac aaagtgtttg agcaggcttt tttcacgatg ataaaccagc
     7861 gtcagttacg ccctagggaa actttttctg aactgcaact ggctcgggcc gcgggtgtca
     7921 gcccggtggt tgtaagagaa tatcttttaa aattcggccg ttataatctt attcaaagcg
     7981 aaaagcgcgg ccagtggagc atgaagcaat tcgatcaatc ctacgccgag cagctgtttg
     8041 agctacgcga aatgctcgaa acacattcac ttcagcactt tctcaacctg cctgatcatg
     8101 atccccgctg gctgcaggcg aaaaccatgc tagaacgtca tcggctactg cgtgacaaca
     8161 tcggcaatag ctttcgcatg ttctcgcaac ttgacagaga ttttcactcc ttactgcttt
     8221 cagctgccga caatatcttt tttgatcaat cgcttgagat catctcagtc atctttcatt
     8281 ttcactatca atgggacgaa agcgatctta aacagcgcaa tatcattgcc gtcgatgaac
     8341 atatgaccat tctcagcgct ctgatttgcc gcagtgacct tgatgccacc cttgcgctcc
     8401 gtaaccattt gaattcagct aaacaatcaa tgattcgatc cattaatgag aacacgcgtt
     8461 atgctcatta agtacggatt aaaaacagca aactattagc gggtgcacgg ttttatcaaa
     8521 agataaatac cgggaaattc ccgcttacct atgctcacaa tcagtcgact aaaaatgttt
     8581 ttgtgagcgg tagtaaagtc ctaaaacttt aacctgccgt taatgtcaac cttgcgtaat
     8641 gcacaattac gtaaacaatt aaaattataa atacaggagt ccggcgtgga aaaagaaaat
     8701 atcaccctcg atccgcgttc ttcatttact ccatcttcgt cggcagatat tcccgtgcca
     8761 ccagatggat tagttcaacg cagtacccga attaaacgca ttcaaaccac cgccatgttg
     8821 ttattatttt ttgcggcggt aatcaattat ctcgaccgca gttcgctgtc ggtagcaaat
     8881 ttaacgattc gtgaagaatt gggattaagt gccaccgaaa tcggcgcttt gctctccgtg
     8941 ttttcactcg cttacggcat tgcgcaactt ccttgcggcc cactattgga tcgtaaaggc
     9001 ccacgcctga tgctgggact ggggatgttc ttctggtcac tgttccaggc catgtctggc
     9061 atggtgcaca gctttacgca gttcgtgttg gtgcgtatcg gtatggggat tggtgaagcg
     9121 ccgatgaacc catgcggtgt aaaagtcatt aacgactggt tcaacatcaa agagcgcgga
     9181 cgcccgatgg gcttcttcaa cgcagcttct accattggcg ttgctgtaag cccaccgatt
     9241 ctggcggcaa tgatgctggt gatgggctgg cgcgggatgt ttattaccat tggtgtactg
     9301 gggatttttc tcgccatcgg ctggtatatg ctctatcgca accgcgagca cgtagaactg
     9361 actgccgttg aacaagctta tctcaatgca ggtagcgtca atgcccgccg agatccgctc
     9421 aattttgccg aatggcgcag cctgttccgt aaccgcacaa tgtggggaat gatgctcgga
     9481 ttcagtggca tcaactacac tgcgtggctg tatctggcct ggcttcctgg ttacctgcaa
     9541 acagcctata acctggattt aaaaagcaca gggttgatgg cggctatccc tttcctgttt
     9601 ggggctgccg ggatgctggt caacggttac gttactgact ggctggtcaa agggggaatg
     9661 gctccgatta aaagccgtaa gatctgcatt attaccggga tgttctgttc tgccgccttt
     9721 acgctgatag taccacaagc gacaacatcc atgatggcgg ttctgctgat tggcatggca
     9781 ctgttctgta ttcactttgc cggaacatcc tgctggggct tgatccacgt cgcagttgct
     9841 tctcgcatga ctgcgtcggt gggcagtatc cagaactttg ccagcttcat ctgcgcctct
     9901 ttcgcgccga tcattactgg ttttattgtt gatacaaccc actcattccg tctggcacta
     9961 atcatttgcg gttgcgtcac cgcagcggga gcactggcgt acatcttcct ggttcgccag
    10021 ccgatcaacg acccgcgtaa agattaaact ccctatgccc gataacattt tgcttatcgg
    10081 gcatttttat ggcgattaaa acgtttccca gttctccccg ctatctgcca ctgccatttt
    10141 acgcggcgta gctggcgtca cggtttttac cacagccgat gtttcacgct gctgttgctg
    10201 ctgttgctga atccggaaca ctgccacagc ttcggtcagg cgactggcct gctcttccag
    10261 cgcggcggcg gcagcggcag attcttccac cagcgcggcg ttctgttgag ttacccggtc
    10321 catctcagca accgctaagc caacctgatc gataccacgg ctctgctcat cagaagcaga
    10381 agatatttcg cccataatgt ccgtcacgcg ggtcacagca ctgacaatct ccgccattgt
    10441 ttccccggcg ctttcgacca gcgtagagcc aacatccact ttccccaccg agtcttcaat
    10501 caggctttta atttcacgaa ccgcctgggc gctacgctgg gccagattac gcacttctcc
    10561 cgcaaccacc gcaaaaccac gtccttgctc acccgcacgc gctgcttcaa ccgccgcgtt
    10621 caaagccaga atattggtct ggaaggtaat gccgtcaatt acgctgataa tatcggcgat
    10681 tttctgcgaa ctggtggaga tatcgcgcat agtctgcacc acgttatcta ccactttgcc
    10741 gccgcgttgc gccgtttcag aagcacttaa tgccagatgg ctggcctggc gcgcattctc
    10801 ggcgttctgt ttcaccgttg cggtcagttg ctccatgctg gctgccgtct cttccagcga
    10861 agcggcctgt tgctcggtgc gcgaagagag atcattattg ccggtagcga tttcgctggc
    10921 accgctatag atggcattgg ccccgttgcg cacatcaccg acggtacgca tcagctctcc
    10981 ctgcatatgg cgcaaactct ctgccagttg ccccatctca ttagagccat ccacctcaat
    11041 cggtttcacc agatcgccgc ctgcaatatg acgaatgctg tcaatcaggc gattcattgg
    11101 cgctaccagc gaggctttaa taccgaacca gacggcgaag atgaccgcca gtacgacgat
    11161 catcacgccc accagaatcc acatcgcctg gctgtaggag gcattgttat cgctgacggc
    11221 gatatcatag agccgatcgt tttgctccat gtaagccaca tactgcttct cgaaaccgtc
    11281 ctgatatccc tgggtcggct gatcaaagaa ctcgttgatt ttgcctgcac ctaacagttg
    11341 gatcagctcc gccagcgcat tgtgataaat atcgtaatta cgtttgatct ctgccgctgc
    11401 ggcggtgctc tgacgcgggt cacgcggcaa cgcttcgtaa tccgcccagt ttttttccgc
    11461 ctgtttcagc gaaatactgg cactctgcat cagctcagca acggttgaac cgctaccaat
    11521 attattctga tccatcatgt agcggatacc cgcgcggttg agggtgttac gcgtctgcaa
    11581 caacgcgacc cagctgccat tcagcgtggg ttgctgctgg cgaatggttt gtaaaacagt
    11641 gaaattttct ttgtcattct ttaaggcatt aaaaaacaga ccgcctgatg tcagttgtaa
    11701 aaggccaaaa acggccaaaa ccagcagtaa gctggtcacg atcttgatac gttttaacat
    11761 ggtttctctt tcctgtggac cgatgcaaga ttttcggcct ggaaagcaaa aactttatga
    11821 aaaggtgtaa cccgccatcc atggaaaaac caggcgctgg ttatcagata ctaaaaagtt
    11881 attcattatc gcccgggatg tcttcttgtc gggaattgcc cagagcaata aattcctcta
    11941 acagatgggt taactgttgc attttctcgg cagtaaactg agcttcaatc tgccgatagg
    12001 cttcttcaat ctgtgtctga gcgcggttat acagcgcctg cccctctttg gtcagcgaga
    12061 tatagagctt ccgctgatca ttgatcggct ttaatcgcaa cactaaaccg tcgcgttcca
    12121 tccgcgtgag gatcccggtc aggcttgggc gcaaaatgca ggcgcgatac gccagatcgt
    12181 gaaaatccat tgatgggctt tccgccagaa tacgcacgat gcgccactgt tgctcagtca
    12241 aattatgccg tttaacgatt gggcgaaaat aactcatcgc cgcttctcgc gcctggagca
    12301 acgcaatggt tagtgagtcg tgcattatct ttcccctgaa aaattcaaaa tcattaatat
    12361 agaaacaatt aatcatcacg ttaccgggac tgagctgccc ccgcaattct tcattttatc
    12421 aaaattgatg atattttatt aacttactga tatttaatat tttattatct ttaaatgtaa
    12481 atagtttgtt aattagatca catttatatc acttactttt gccaaactac gcaatcggga
    12541 ataaaagtaa tcattaacat attaatgatt aagatgatcg aatccgagga gtggtaatga
    12601 aaggcactat cttcgccgta gcgttgaacc atcgcagcca acttgatgcc tggcaggaag
    12661 cgttccagca atctccctac aaagccccgc ctaaaactgc ggtctggttt attaaaccgc
    12721 gcaatacggt gattggttgc ggtgaaccga ttccctttcc acagggtgaa aaagtactga
    12781 gcggtgcgac tgttgcgctg attgtgggga aaacggcgac gaaagtacgt gaagaagatg
    12841 cggcagagta catcgccgga tatgcgctgg ataacgacgt cagcctgccg gaagagagct
    12901 tttaccgtcc ggcaatcaaa gcaaaatgtc gtgatggatt ctgccccatt ggcgaaaccg
    12961 tggctctcag caatgtcgat aatctgacta tctataccga gatcaacggg cgtcctgccg
    13021 atcactggaa taccgccgat ttacaacgta acgccgcaca gctgctgagc gccctgagcg
    13081 aatttgccac gctgaatcca ggcgatgcta ttctgctcgg cacgccacag gcgcgcgtgg
    13141 aaatacagcc aagcgatcgc gtgcgtgttc tcgcagaagg tttcccgccg ctggaaaatc
    13201 cggtagtgga cgaacgtgaa gtgaccacgc gcaagagctt cccaacgctg ccacacccgc
    13261 acggtacgct gtttgccctc ggcctgaact acgccgacca cgccagcgaa ctggaattta
    13321 agccaccgga agagccgctg gtgttcctga aagcgccgaa taccctcact ggcgacaacc
    13381 agacctccgt gcgcccgaac aatattgaat acatgcacta cgaagcggag ctggtggtag
    13441 ttattggcaa gcaggcgcgt aacgtcagcg aagccgatgc catggattat gtcgcgggct
    13501 acaccgtgtg taacgactac gccattcgcg actatctgga aaactactac cgccctaacc
    13561 tgcgggtcaa aagccgcgac ggactgacgc cgatgctttc aaccatcgtg ccgaaagagg
    13621 cgatcccgga cccgcataat ctgacccttc gcaccttcgt caacggcgag ttacgccagc
    13681 aaggcaccac cgccgatctg atcttcagcg tgcccttcct gatcgcctac ttaagcgaat
    13741 ttatgaccct gaatccgggc gacatgatcg ccaccggcac accaaaaggc ttatctgacg
    13801 tggtgcctgg cgatgaagta gtggtggaag tagaaggcgt gggccgcctg gtgaaccgaa
    13861 ttgtgagtga ggaaacagcg aaatgaaaaa agtaaatcat tggatcaacg gcaaaaatgt
    13921 tgcaggtaac gactacttcc agactaccaa tccggcaacg ggtgaagtgc tggcggatgt
    13981 ggcctctggc ggtgaagcgg agatcaatca ggcggtagcg gcagcgaaag aggcgttccc
    14041 gaaatgggct gatctgccga tgaaagagcg tgcgcgcctg atgcgccgtc tgggcgatct
    14101 gatcgaccag aacgtgcctg agatcgccgc gatggaaacc gcggacacgg gcctgccgat
    14161 ccatcagacc aaaaatgtgt tgatcccacg cgcttctcac aactttgaat ttttcgcgga
    14221 agtctgccag cagatgaacg gcaagaccta tccggttgac gacaagatgc tcaactacac
    14281 gctggtgcaa ccggtaggcg tttgtgcgct ggcgtcgccg tggaacgtgc cgtttatgac
    14341 cgccacctgg aaggtcgcgc cgtgtctggc gctgggcaat accgcggtgc tgaagatgtc
    14401 ggaactctcc ccgctgaccg ccgaccgcct gggtgagctg gcgctggaag ctggtattcc
    14461 ggcgggcgtg ctaaacgtgg tacagggcta cggcgcaacc gcaggcgatg cgctggtccg
    14521 tcatcatgac gtgcgtgccg tgtcgttcac cggcggtacc gccaccgggc gcaacatcat
    14581 gaaaaacgcc gggctgaaaa aatactccat ggagctgggc ggtaaatcac cggtgctgat
    14641 ttttgaagat gccgatattg agcgtgcgct ggacgccgcc ctgttcacca tcttctcaat
    14701 caacggcgaa cgctgcaccg ccggttcgcg catctttatt cagcagagca tctatccaga
    14761 attcgttaaa cgctttgccg aacgcgccaa ccgtctgcgc gtgggcgatc cgaacgatcc
    14821 gaatacccag gttagggcgc ttatcagtca gcaacactgg gaaaaagtct ccggctatat
    14881 ccgtctcggc attgaagaag gcgcaaccct gttggcgggc ggcccggata aaccgtccga
    14941 cctgcctgcg cacctgaaag gcggcaactt cctgcgccca acggtgctgg cggacgtaga
    15001 taaccgtatg cgcgttgccc aggaagagat tttcgggccg gtcgcctgcc tgctgccgtt
    15061 taaagacgaa gccgaaggct tacgcctggc aaacgacgtg gagtacggcc tcgcgtcgta
    15121 catctggaca caggatgtca gcaaagtgtt acgcctggcg cgtggcattg aagcaggcat
    15181 ggtgttcgtc aacacccaga acgtgcgcga cctgcgccag ccatttggcg gcgtaaaagc
    15241 ctccggcacc gggcgtgaag gcggtgagta cagcttcgaa gtgttcgcgg aaatgaagaa
    15301 cgtctgcatt tccatgggcg accatccaat tccgaaatgg ggagtctgat atgggtaagt
    15361 tagcgttagc cgccaagatc acgcacgtac cgtcgatgta tctctctgaa ctaccaggaa
    15421 aaaaccacgg ttgtcgccag ggtgcgatcg acgggcataa agagatcagc aagcgttgcc
    15481 gggaaatggg cgtcgatacc attatcgttt tcgatactca ctggctggtc aacagtgctt
    15541 atcacatcaa ctgtgcagac cattttgaag gcgtctacac cagcaacgag ctgccgcatt
    15601 ttattcgcga catgacctac aactacgagg gcaacccgga gttggggcag ctaattgccg
    15661 aggaagcctt aaagctcggc gtgcgggcaa aagcgcacaa tattcccagc ctgaaactgg
    15721 aatacggcac gctggtaccg atgcgctaca tgaatgaaga caagcacttc aaagtggtct
    15781 ccatttcggc tttctgcacg gttcacgatt ttgccgacag ccgcaagctg ggcgaagcga
    15841 ttctgaaagc gatcgaacag tacgacggca ccgtggcggt ccttgccagc ggttcgttat
    15901 cgcaccgctt tattgacgat cagcgtgcgg aagaagggat gaacagctac acccgcgagt
    15961 tcgaccgcca gatggacgag cgtgtggtga agctgtggcg cgaaggccag ttcaaagagt
    16021 tttgcaatat gctgccggag tacgccgact actgctacgg cgaaggcaat atgcacgaca
    16081 cggtgatgct gctggggatg ctcggctggg ataaatacga cggcaaggtg gagtttatta
    16141 ccgagctgtt cccaagctct ggcaccggtc aggttaacgc tgttttcccg ctgcccgcgt
    16201 aaggacgttt tatgccgcac tttatcgtgg aatgtactga aaatattcgt gaagaagccc
    16261 gcttacctga gctgtttgcc agtgttaaca ctgcactggc ggcaacgggg atttttccgc
    16321 ttgggggaat acgtagccgg gcgcactgga tcgatacctg gcaaatggct gatggcaagc
    16381 acgattacgc ctttgtgcat atgacgctaa aaatcggcag cggacgcagt ctggaaagcc
    16441 gtcaggaagt gggcgaaatg ctgtttgatc tgattaaaac gcacttcgcc tcactgatgg
    16501 agagccgtta cctagcgctt tcatttgaga ttgcagagct gcacccgacg ttgaatttca
    16561 aacagaacaa cgtacacgcg ttgtttaaat aaatttgcac cagcttccag gccggatgat
    16621 aaggcgctgc cgcatccggc aatgccacag gatatcgcta tgttcgataa acacacccac
    16681 accctgatcg cccagcgtct ggatcaggca gaaaaacagc gcgaacagat ccgcgcgatc
    16741 tcgctggatt acccggagat caccatcgaa gacgcctacg ccgtgcagcg cgaatgggtt
    16801 cgcctgaaaa tcgccgaaag tcgcacgctg aaaggccaca aaatcggtct gacctcgaaa
    16861 gcgatgcagg caagctcgca gatcagcgaa ccggattacg gtgcactgct ggacgacatg
    16921 ttcttccacg atggcagcga tatcccgacc gatcgcttta tcgtgccgcg cattgaagtg
    16981 gagctggctt ttgtgctggc aaaaccgctg cgtgggccaa actgcacgct gttcgacgtt
    17041 tacaacgcca cggactatgt gatcccggcg ctggagctga tcgacgctcg ctgccacaac
    17101 atcgatccgg aaacccagcg cccgcgcaaa gtattcgaca ccatttccga taacgccgcc
    17161 aacgccggag tgatcctcgg aggtcgtccg atcaaaccag acgaactgga tctgcgttgg
    17221 atctccgccc tgatgtatcg caatggcgtg attgaagaaa ccggcgtcgc cgctggtgtg
    17281 ctgaatcatc cggcaaacgg cgtggcctgg ctggcgaaca aactcgcacc gtatgacgta
    17341 caactggaaa ccgggcaaat cattctcggt ggttcgttca ccctcccggt tccggcgcgt
    17401 aagggcgaca ccttccacgt cgattacggc aacatgggct ccattagctg ccgctttgtt
    17461 taaggagaga acgatggaaa acagttttaa agcggcgctg aaagcaggcc gtccgcagat
    17521 tggattatgg ctggggctga gcagcagcta cagcgcggag ttactggccg gaccaggatt
    17581 cgactggtta ttgatcgacg gtgagcacgc gccgaataac gtgcaaaccg tgctcaccca
    17641 actacaggcg attgcgccct accccagcca gccagtggta cgcccatcgt ggaacgatcc
    17701 ggtgcaaatc aaacaactgc tggacgtagg cacacaaacc ttactggtgc cgatggtaca
    17761 aaacgccgac gaagcccgta aagcggtacg cgccacccgt tatccccccg ccggtattcg
    17821 cggtgtgggc agtgcgctgg ctcgcgcctc gcgctggaat cgcattcctg attacctgca
    17881 aaaagccaac gatcaaatgt gcgtgctggt gcagatcgaa acgcgtgagg caatgaagaa
    17941 cttaccgcag attctggacg tggaaggggt cgacggcgtg tttatcggcc cggcggatct
    18001 gagcgccgat atgggttatg ccggtaatcc gcagcacccg gaagtacagg ccgccattga
    18061 gcaggcgatc gtgcagatcc gcgaagcggg caaagcgccg gggatcctga tcgccaatga
    18121 gcaactggca aaacgctatc tggaactggg cgcgctgttt gtcgccgtcg gcgttgacac
    18181 caccctgctc gcccgcgccg ccgaagcgct ggcagcacgg tttggtaata gttcttctat
    18241 ttcgatggcg aagcaaaaca acaactctgt ctactaaacg tacgatgtgg agcgcatcat
    18301 gacgacctct accctgcaat ataaagataa taaaactgtt gaaattgaac atcgtgtcat
    18361 taataaatta tttcgtcgcc taatcatttt tctttttatt ctgtttgttt tttcgtttct
    18421 tgatcgcatc aatataggat ttgccggact gacaatgggc aaggatcttg gcctgacgtc
    18481 cacaatgttt ggcctcgccg caacactctt ttatgtcacg tacgtcatgt gcgggatccc
    18541 cagtaatatg atgttagcga aaattggcgc tcgtcgatgg atcgctggga tcatggtggt
    18601 ctggggaatt gcttccacct gcacaatgtt tgcaacaagc cccgaaacac tctatctact
    18661 gcgtatgctg gttggtattg ctgaggcggg ttttttacct ggtattttgg tttatctgac
    18721 ctggtggttc ccggcatatc atcgggccag agccaatgcc ttatttatga tagcgatgcc
    18781 agtcactatg atgctgggat tgatcctttc aggctacatt ctggccttgg atggactgtg
    18841 gaatctaaaa ggctggcaat ggctcttttt gctggaaggt ctgccatcgg tcattcttgg
    18901 tgtcgtcacc tggttttatc tcaacgacac accagaccag gcgacctggc ttgatgaaaa
    18961 cgaaaaacag gctttaaaaa cgatgattct ccgtgaacaa gagagtgcat ttgcccatgc
    19021 atctgttcca cgctcaacct tacgtgaagt cctgacccca gccatcatgc tatatacact
    19081 tgcttatttt tgcccgacta atactctgag tgcgattaat atctggacgc cgcaaatcct
    19141 acagagtttc aatactggca gtagcaacat catgattggt ttgctggcgg ccatccccca
    19201 attctgcaca atttttggca tgatctggtg gagtcgtcgt tccgaccgtc ttaaagaacg
    19261 caaaaagcat acgattcttc cctacctgtt tgccgctgcc ggatggttac tcgcttctgc
    19321 gacagatcac agcctcattc aactgagcag catcattatg gcctcaatgg gatcatttac
    19381 cgcgatgact atcttttgga caacaccgga tcaagttata agcttgcaat cgcgtgccgt
    19441 tgcgatggct gtcatcaatg ctgtcggcaa tgtaggttca gcagtcagtc cattacttat
    19501 cggtattttg aaagatgcaa ccggcagttt tagctcaggt ttgtggtttg tagcaggatt
    19561 gctggtactg ggcgcgttag tcatgacccg aatccctatg gggagtcggg aacactctct
    19621 ttcatctgaa cttatcgcac caaaaccgca ataaggagtc atgatgtcca gtgaagctat
    19681 ctttgaacat ggttatgaaa atattgattt ggtccgggaa tatgatgccc gctacgctgg
    19741 tgaagatgtg cattatgaaa catttgcccg gttagcgaat ttctttggac gcgatatgcg
    19801 ccctcactgg cacgatagat attttcaact ccattatctg gtcactggac gcattacttt
    19861 acaactggat gagcacttct attctgtgcg cgcaccgctt tttgtactaa caccaccatc
    19921 ggtgccacat actttcttta ctgatcctga tagcgatggg catgttctga ccgtacgtca
    19981 ggagttaatt tggcccctgg tagaaaagct ctggcctggt agtggagatg ccgcaatgct
    20041 acaaggtatc tgtctgtcac tggagggaat ggaacataca ctggcggcat taaatcatta
    20101 ttggccgata atatccctgg agtttcaaca aaataagcaa ggacgggaaa ttctattgca
    20161 atcactggcg caaagtattt ttacaatcct actgcgtgaa gcaccaccaa atgatatttc
    20221 aacatgcagc gtgcgcggcg agatgtgttt attccagaag tttaatcggc tgattgacga
    20281 aaattatcgc caacatttaa tggtccctga atatgcaaca atgctcggta tgagtgaatc
    20341 acggcttacc gagctgtgcc gacgttttgc caatcaatcg ccgaagagat tgattttcga
    20401 acgagtgagc cgggaagcca gacggcttct gctctatagc gagcaaagca tcaaccagat
    20461 agcccttgat cttggctaca aagatcccgc ctatttcgcc cgttttttca accgaatgat
    20521 gggttgctca ccaacacaat tccgtggtcg ctgaccattt atcaacctga gagactcgac
    20581 atgaaaatat ctttacgctc actatttgtg gcgtgcctga ttgcgccagt ggcattcacc
    20641 tacgcctccg aaccgcaata aatgaaaatt gtactacttg gaacgaaagg tggcccttct
    20701 ttattaaaag ctgaccgttt accgatggca acggcactta ttattggcaa taaaacctgg
    20761 ttaattgatg ctggttacgg ggccagctta caactggtaa aaaataatat tccactgcgc
    20821 cagattgata atattttact cacccattta cacagtgatc atattctgga ctatccatca
    20881 gtcttaatga atgcatgggc tagcggtctg caggacaaaa ctattcatgt attcggtccg
    20941 ccaggcaccc gagccatgac cgctgccagt tggcaggtct tcgatcggga catcacactg
    21001 cgtatggaag aagaaggaaa acccgatcct cgcaaactcg ttacagctca agatgttggt
    21061 gaaggcgtag tttataaaga tgataccgtg accgtgactg cattaaaggt ccctcattct
    21121 cctttccctg acggtgaggc ttttgcttat cgcttcgaca cacaaggcaa gcgaattgtc
    21181 ttctcaggag atacctcgta ctttccccca ctggcaactt tctcagaagg cgctgatata
    21241 cttgtgcatg aggccgtaca tgagccctcc gtcgctaagc tggcagacag tattggcaat
    21301 ggtaaaacac tggcaaaagc cattgcttct caccatacga ctattgagga tgtaggcaaa
    21361 attgccaatg aagcaaaagt gaaaaaactc atccttagcc acctggtacc aatcaccgtt
    21421 ccggatgaaa catggaagca agaagcacag aaaactattc aggtcctatc gtcgttggtc
    21481 atgaaaatat gacgattacc attccattcg ccccataaaa aattccgtcc ccgttagccc
    21541 ttgttagcgg ggaatattat gatttgcctt actgtaagtt gatcacgatc gcagtttact
    21601 atttaactcc accttgctgt cgaaaagtac caacgttcgc ctgaaacgtc tcttcccgac
    21661 aaccgcgttc aacgcttcaa ttaaataaca aaaacaacac acaaaattaa caatcgtatt
    21721 ccgattaata ctgtagaggt cgacatgaaa ccagaagatt tccgcgccag tacccaacgt
    21781 cctttcaccg gggaagagta tctgaaaagc ctgcaggatg gtcgcgagat ctatatctat
    21841 ggcgagcgag tgaaagacgt caccactcat ccggcatttc gtaatgcggc agcgtctgtt
    21901 gcccaactgt acgacgcgct gcacaaaccg gagatgcagg actctctgtg ttggaacacc
    21961 gacaccggca gcggcggcta tacccataaa ttcttccgcg tggcgaaaag tgccgacgac
    22021 ctgcgccagc aacgcgacgc catcgctgaa tggtcacgcc tgagctatgg ctggatgggc
    22081 cgtaccccag actacaaagc cgctttcggt tgcgcactgg gcgcgaatcc gggcttttac
    22141 ggtcagttcg agcagaacgc ccgtaactgg tatacccgta ttcaggaaac tggcctctac
    22201 tttaaccacg cgattgttaa cccaccgatc gtcatttgcc gaccgataaa gtgaaagacg
    22261 tttacatcaa gctggaaaaa gagactgacg ccgggattat cgtcagcggt gcgaaagtgg
    22321 ttgccaccaa ctcggcgctg actcactaca acatgattgg cttcggctcg gcacaagtga
    22381 tgggcgaaaa cccggacttc gcactgatgt tcgttgcgcc aatggatgcc gatggcgtga
    22441 aattaatctc ccgcgcctct tatgagatgg tcgcgggtgc taccggctcg ccatacgact
    22501 acccgctctc cagccgcttc gatgagaacg atgcgattct ggtgatggat aacgtactga
    22561 tcccatggga aaacgtgctg atctaccgcg attttgatcg ctgccgtcgc tggacgatgg
    22621 aaggcggttt cgcccgtatg tatccgctgc aagcctgtgt gcgcctggca gtgaaactcg
    22681 acttcattac ggcactgctg aaaaaatcac tcgaatgtac cggcaccctg gagttccgtg
    22741 gtgtgcaggc cgatctcggt gaagtggtgg cgtggcgcaa caccttctgg gcattgagtg
    22801 actcgatgtg ttcagaagcg acgccgtggg tcaacggggc ttatttaccg gatcatgccg
    22861 cactgcaaac ctatcgcgta ctggcaccaa tggcctacgc gaagatcaaa aacattatag
    22921 aacgcaacgt taccagtggc ctgatctacc tcccttccag tgcccgtgac ctgaacaatc
    22981 cgcagatcga ccagtatctg gcgaagtatg tgcgcggttc gaacggtatg gatcacgtcc
    23041 agcgcatcaa gatcctcaaa ctgatgtggg acgccattgg cagcgaattt ggtggtcgtc
    23101 acgagctgta tgaaatcaac tactccggta gccaggatga gattcgcctg caatgtctgc
    23161 gccaggcaca aagctccggc aatatggaca agatgatggc gatggttgat cgctgcctgt
    23221 cggaatacga ccagaacggc tggactgtgc cgcacctgca caacaacgac gatatcaaca
    23281 tgctggataa gctgctgaaa taacgcagca ggaggttaag atgcaattag atgaacaacg
    23341 cctgcgcttt catgacgcga tggccagcct gtcggcagcg gtaaatatta tcaccaccga
    23401 gggcgacgcc ggacaatgcg ggattacggc aacggctgtc tgctcggtca cggatacacc
    23461 accgtcgctg atggtgtgca ttaacgccaa cagtgcgatg aacccggttt ttcagggcaa
    23521 cggcaagttg tgcgtcaacg tcctcaacca tgagcaggaa ctgatggcgc gccacttcgc
    23581 gggcatgaca ggcatggcga tggaagagcg ttttagcctc tcatgctggc aaaaaggtcc
    23641 gctggcgcag ccggtgctaa aaggttcgct ggccagtctt gaaggagaga tccgcgatgt
    23701 gcaggcgatt ggcacacatc tggtgtatct ggtggagatt aaaaacatca tcctcagtgc
    23761 agaaggtcac ggacttattt actttaaacg ccgtttccat ccggtgatgc tggaaatgga
    23821 agctgcgatt taagctccag ccccttcacg cattcgtcga aggggcattt ttattgtgat
    23881 ctcaatcaca tatctacccg atgaataatt cttgcccaac cgctcaccgc cattcctgac
    23941 cgcttacatc cctaaaataa ccactcagtt atttacctta ctttacgcgc acgtatctct
    24001 ggcaacatca ctgcaggata gcggtcaatt tacctcctca aacgcaacgc aaacctagaa
    24061 cggcttcggc ctatgattaa caaattactc tatggatttt ggtttgcagg aaggcagcaa
    24121 gtgagtgaat ccccgggagc ttacaaaagt aagtgtcaga ggtgaacgaa cgcagctgcc
    24181 gcacctgtaa gccaaaagac gacgagtaaa atgccaggtt ttactatgga tacgaaaaaa
    24241 ctattcaagc acataccctg ggtgattctc ggaatcatcg gtgcattctg cctcgcggta
    24301 gttgcattac gtcgggggga gcacgtcagc gccctgtgga tcgtggtcgc ctctgtgtcg
    24361 gtgtatctgg tggcgtatcg ctactacagt ctgtacatcg cccagaaggt gatgaaactc
    24421 gaccccacgc gcgcgacgcc tgcggttatt aacaacgacg gtctgaacta cgttccgacc
    24481 aaccgttacg tgttgtttgg tcaccacttc gccgctatcg ccggtgctgg tccgctggtg
    24541 ggaccggttc tcgccgcgca gatgggctac ctgcctggca cgctgtggct gctggcgggg
    24601 gtagtgctgg ccggtgcggt acaggacttt atggtgctgt ttatctcctc tcgccgtaac
    24661 ggcgcatctc tcggtgagat gatcaaagaa gagatggggc cagtaccggg gactatcgcg
    24721 ctgtttggct gtttcttaat catgatcatc atcctcgccg ttctggcgct gattgtggtt
    24781 aaagccctgg ccgaaagtcc gtggggtgtc ttcaccgttt gctcaaccgt accgattgcg
    24841 ctgtttatgg gtatctacat gcgctttatc cgtccggggc gtgtgggtga agtctctgtt
    24901 attggtatcg tgctgctggt tgcctctatc tacttcggtg gcgtgattgc tcacgatccg
    24961 tactggggtc cggcactgac ctttaaagac accaccatta ccttcgcgct gattggctat
    25021 gcgtttgttt ccgcactgct gccggtgtgg ctgatcctcg caccgcgcga ctatctggca
    25081 accttcctga aaatcggcgt tatcgtcggc ctggcgctgg gtatcgtggt gctgaacccg
    25141 gaactgaaaa tgcctgcgat gacccagtac attgacggta ctggcccgct gtggaaaggc
    25201 gctctgttcc cgttcctgtt catcaccatc gcctgtggtg cggtatctgg cttccacgcg
    25261 ctgatctctt ccggtacgac gccgaaactg ctggctaacg aaaccgacgc gcgtttcatt
    25321 ggctacggcg caatgctgat ggagtccttc gtggcgatta tggcgctggt tgcagcgtcc
    25381 atcatcgaac cgggtcttta cttcgcgatg aacaccccgc ctgctggcct tggcatcacc
    25441 atgcctaacc tgcatgaaat gggtggcgag aacgcgccga tcatcatggc gcagctgaaa
    25501 gacgttaccg cacacgcggc agcgaccgtc agctcctggg gcttcgtgat ttcgccagag
    25561 cagatcctgc aaaccgcgaa agacattggt gagccttctg tcctgaaccg tgcaggtggt
    25621 gcgccgacgc tggcggtagg tatcgctcac gtgttccaca aagtgctgcc gatggctgac
    25681 atgggcttct ggtatcactt cggtattctg ttcgaagccc tgttcatcct gaccgcgctg
    25741 gatgcgggta cccgttctgg ccgctttatg ctgcaagacc tgctgggtaa cttcatcccg
    25801 ttcctgaaaa aaaccgactc tctggttgcc ggtatcatcg gtactgcggg ctgtgtgggt
    25861 ctgtggggct acctgctgta tcagggcgtg gtcgatccgc tgggcggcgt taagagcctg
    25921 tggccgctgt tcggtatctc caaccagatg ctggcagccg tagcgctggt actgggcacc
    25981 gttgtgctga ttaagatgaa gcgcacccaa tacatctggg taactgttgt tccggctgta
    26041 tggctgctta tctgcaccac ctgggcgctg ggtctgaaac tgttcagcac caacccgcag
    26101 atggaaggct tcttctacat ggcaagccag tacaaagaga agattgctaa cggtactgac
    26161 ctgacggcgc agcagattgc caacatgaac cacatcgttg tgaacaacta caccaacgca
    26221 ggcctgagta ttctgttcct gattgtggtg tacagcatca tcttctacgg tttcaaaacc
    26281 tggcttgcgg tgcgtaacag cgacaaacgt actgacaaag aaacaccgta cgttccaatc
    26341 ccggaaggcg gcgtgaagat ctcttcgcac cactaaccgt gtttagcccc gcttcggcgg
    26401 ggctttgttc ttatcagagt gaactatgtt tggtaactta ggacaggcaa aaaaatatct
    26461 cggccaggcg gcgaagatgt tgattggtat tccagactac gacaactacg tcgagcatat
    26521 gaagaccaac catcccgaca agccgtacat gagctatgaa gaattcttcc gcgaacgcca
    26581 gaacgcgcgc tacggcggcg atggtaaagg cggtatgcgc tgttgttaat ggagagagca
    26641 tgaacccgat tgcagttacc ctactcaccg gttttttagg cgcgggcaaa actaccctgc
    26701 tgcgccatat tcttaacgaa caacacggct acaagattgc cgtgattgaa aacgaattcg
    26761 gcgaagtctc cgttgatgac caactgattg gcgaccgcgc cacgcagatc aaaacgctga
    26821 ccaacggctg catctgctgt tcgcgctcca acgagctgga ggacgcacta ctcgacctgc
    26881 tggataatct cgacaagggc aatattcagt tcgatcgtct ggtgattgaa tgcaccggca
    26941 tggccgatcc cggcccgatt attcagacct ttttctccca tgagatttta tgccagctct
    27001 acctgctgga cggcgtgatt gcgctggtgg atgcggtaca tgccgatgag cagatgaacc
    27061 agttcaccat cgcccagtcc caggtgggct acgccgaccg tattctgctg actaaaaccg
    27121 acgtcgcagg cgaagcagag aaactgcgtg aacgcctggc gcgcatcaac gcccgcgcac
    27181 cggtctacac cgtcacccac ggcgacatcg acctgggcct gctgttcaac accaacggtt
    27241 ttatgctgga agagaacgtc gtcagcacca aaccgcgttt ccactttatc gcggataaac
    27301 aaaacgatat ttcgtcaatt gtggtggaac tggattaccc ggtagatatc agcgaagttt
    27361 cccgcgtgat ggaaaacctg ctgctggaat cggcggataa actgctgcgt tacaaaggga
    27421 tgctgtggat tgacggcgaa cctaaccgcc tgctgttcca gggcgtccag cgcctctaca
    27481 gcgccgactg ggacaggccg tggggcgatg aaaaaccgca tagcacaatg gtgtttatcg
    27541 ggattcagtt gccggaggaa gagattcggg cggcgtttgc gggattgagg aagtaagttc
    27601 gggaaagatc aactttttga gagttccacc gcaacattat tacgagtgga actctttaga
    27661 tatgtcagac caaccaaagc ttcttcaatt gcgttttcat tttttcatgt ctcattttca
    27721 gttcagccaa tccccattct gaagaattac aaacatcttt tgttaaataa aaagagcttt
    27781 tactattaag tttctcataa atagatttct ttttgataaa atcatagtgt tgtgtttcag
    27841 aatttttgga accacttatg agtgttaaat tacctatttt atgcagccag tagacatgct
    27901 cttgaggttg gaatctatta atccagtatt cattaactaa tgcttgagga agaatatgct
    27961 caattgtgat tctccccgta taggttttaa tcacgctctc atcttgttgc tccatatcta
    28021 agcgaagcaa aattgctttt attaagttca cttgattcgg acgaggctca tataaatctt
    28081 catccagagc agctataaaa cctgaattat ctgcatgttg gtttatctga tttatcacag
    28141 aatcaaaagg catatcatta tttattgcta ccagagcaga ataacaaacc atttctcttt
    28201 ggcttttaat ttgttttttt aaccacccat gcatatatac cttctcaaag gcggtaataa
    28261 attgagaaaa atcatctaaa ttaaaatctt ctgtacgagc cattcgattc atgaatgcca
    28321 taaccggagg aatccactca tctaccccta aattacttag cgatgctatt tttcgtcgta
    28381 ttgaagggtg ttcaaaatca ttctctaaaa tcttagtata
//
LOCUS       DAJTRC010000052        28404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.052, whole genome shotgun sequence.
ACCESSION   DAJTRC010000052 DAJTRC010000000
VERSION     DAJTRC010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.052"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="OO357_002343"
     CDS             complement(<1..140)
                     /gene="yjdI"
                     /locus_tag="OO357_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S mono-cluster protein YjdI"
                     /protein_id="HCR5887262.1"
                     /translation="MDQALLDGGYRCYTGEKIDVYFNTAICQHSGNCVRGNGKLFNLK
                     RKS"
     gene            321..1952
                     /gene="dcuS"
                     /locus_tag="OO357_002344"
     CDS             321..1952
                     /gene="dcuS"
                     /locus_tag="OO357_002344"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     DcuS"
                     /protein_id="HCR5887263.1"
                     /translation="MRHSLPYHILRKRPMKLSTTVILMVSAVLFSVLLVVHLIYFSQI
                     SDMTRDGLANKALAVARTLADSPEIRQGLQKKPQESGIQAIAEAVRKRNDLLFIVVTD
                     MQSLRYSHPEAQRIGQPFKGDDILNALNGEENVAINRGFLAQALRVFTPIYDENHKQI
                     GVVAIGLELSRVTQQINDSRWSIIWSVLFGMLVGLIGTCILVKVLKKILFGLEPYEIS
                     TLFEQRQAMLQSIKEGVVAVDDRGEVTLINDAAQELLNYRKSQDDEKLSTLSHSWSQV
                     VDVSEVLRDGTPRRDEEITIKDRLLLINTVPVRSNGVIIGAISTFRDKTEVRKLMQRL
                     DGLVNYADALRERSHEFMNKLHVILGLLHLKSYKQLEDYILKTANNYQEEIGSLLGKI
                     KSPVIAGFLISKINRATDLGHTLILNSESQLPDSGSEDQVATLITTLGNLIENALEAL
                     GPEPGGEISVTLHYRHGWLHCEVNDDGPGIAPDKIDHIFDKGVSTKGSERGVGLALVK
                     QQVENLGGSIAVESEPGIFTQFFVQIPWDGERSNR"
     gene            1949..2668
                     /gene="dcuR"
                     /locus_tag="OO357_002345"
     CDS             1949..2668
                     /gene="dcuR"
                     /locus_tag="OO357_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709817.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator DcuR"
                     /protein_id="HCR5887264.1"
                     /translation="MINVLIIDDDAMVAELNRRYVAQIPGFQCCGTASTLEKAKEIIF
                     NSDAPIDLILLDIYMQKENGLDLLPVLHNARCKSDVIVISSAADAATIKDSLHYGVVD
                     YLIKPFQASRFEEALTGWRQKKMALEKHQYYDQAELDQLIHGSSSNEQDPRRLPKGLT
                     PQTLRTLCQWIDAHQDYEFSTDELANEVNISRVSCRKYLIWLVNCHILFTSIHYGVTG
                     RPVYRYRIQAEHYSLLKQYCQ"
     gene            3239..4579
                     /gene="dcuB"
                     /locus_tag="OO357_002346"
     CDS             3239..4579
                     /gene="dcuB"
                     /locus_tag="OO357_002346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuB"
                     /protein_id="HCR5887265.1"
                     /translation="MLFTIQLIIILICLFYGARKGGIALGLLGGIGLVILVFVFHLQP
                     GKPPVDVMLVIIAVVAASATLQASGGLDVMLQIAEKLLRRNPKYVSIVAPFVTCTLTI
                     LCGTGHVVYTILPIIYDVAIKNNIRPERPMAASSIGAQMGIIASPVSVAVVSLVAMLG
                     NVTFDGRHLEFLDLLAITIPSTLIGILAIGIFSWFRGKDLDKDEEFQKFISVPENREY
                     VYGDTATLLDKKLPKSNWLAMWIFLGAIAVVALLGADSDLRPSFGGKPLSMVLVIQMF
                     MLLTGALIIILTKTNPASISKNEVFRSGMIAIVAVYGIAWMAETMFGAHMSEIQGVLG
                     EMVKEYPWAYAIVLLLVSKFVNSQAAALAAIVPVALAIGVDPAYIVASAPACYGYYIL
                     PTYPSDLAAIQFDRSGTTHIGRFVINHSFILPGLIGVSVSCVFGWIFAAMYGFL"
     gene            4657..6303
                     /gene="fumB"
                     /locus_tag="OO357_002347"
     CDS             4657..6303
                     /gene="fumB"
                     /locus_tag="OO357_002347"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fumarate hydratase"
                     /protein_id="HCR5887266.1"
                     /translation="MSNKPFIYQAPFPMGKDNTEYYLLTSDYVSVADFDGETILKVEP
                     EALTLLAQQAFHDASFMLRPAHQKQVAAILHDPEASENDKYVALQFLRNSEIAAKGVL
                     PTCQDTGTAIIVGKKGQRVWTGGGDEEALSKGVYNTYIEDNLRYSQNAALDMYKEVNT
                     GTNLPAQIDLYAVDGDEYKFLCVAKGGGSANKTYLYQETKALLTPGKLKNFLVEKMRT
                     LGTAACPPYHIAFVIGGTSAENNLKTVKLASAHYYDELPTEGNEHGQAFRDVQLEQEL
                     LEEAQKLGLGAQFGGKYFAHDIRVIRLPRHGASCPVGMGVSCSADRNIKAKINREGIW
                     IEKLEHNLGQYIPQELRQAGEGEAVKVDLNRPMKEILAQLSQYPVSTRLSLTGTIIVG
                     RDIAHAKLKELIDAGKELPQYIKDHPIYYAGPAKTPAGYPSGSLGPTTAGRMDSYVDL
                     LQSHGGSMIMLAKGNRSQQVTDACHKHGGFYLGSIGGPAAVLAQQSIKHLECVAYPEL
                     GMEAIWKIEVEDFPAFILVDDKGNDFFQQIVNKQCANCTK"
     gene            6426..7055
                     /locus_tag="OO357_002348"
     CDS             6426..7055
                     /locus_tag="OO357_002348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2238 domain-containing protein"
                     /protein_id="HCR5887267.1"
                     /translation="MTRTLKPLILNTGALALTLILIYTGISAHDKLTWLLEVTPVIIV
                     VPLLLATARRYPLTLLLYTLIFFHAIILMVGGQYTYAKVPVGFEVQEWLGLSRNPYDK
                     LGHFFQGLVPALVAREILVRGMYVRGRKMVAFLVCCVALAISAMYELIEWWAALAMGQ
                     GADDFLGTQGDQWDTQSDMFCALLGALTTVILLARFHCRQLRRYGLITG"
     gene            complement(7194..8615)
                     /gene="melB"
                     /locus_tag="OO357_002349"
     CDS             complement(7194..8615)
                     /gene="melB"
                     /locus_tag="OO357_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="melibiose:sodium transporter MelB"
                     /protein_id="HCR5887268.1"
                     /translation="MSISMTTKLSYGFGAFGKDFAIGIVYMYLMYYYTDVVGLSVGLV
                     GTLFLVARIWDAINDPIMGWIVNATRSRWGKFKPWILIGTLANSVILFLLFSAHLFEG
                     TTQIVFVCVTYILWGMTYTIMDIPFWSLVPTITLDKREREQLVPYPRFFASLAGFVTA
                     GVTLPFVNYVGGGDRGFGFQMFTLVLIAFFIVSTIITLRNVHEVFSSDNQSSAEGSHL
                     TLKAIVALIYKNDQLSCLLGMALAYNVASNIITGFAIYYFSYVIGDADLFPYYLSYAG
                     AANLVTLVFFPRLVKSLSRRILWAGASILPVLSCGVLLLMALMSYHNVVLIVIAGILL
                     NVGTALFWVLQVIMVADTVDYGEYKLHVRCESIAYSVQTMVVKGGSAFAAFFIAVVLG
                     MIGYVPNVEQSTQALLGMQFIMIALPTLFFMVTLILYFRFYRLNGDTLRRIQIHLLDK
                     YRKVPPEPVHADIPVGAVSDVKA"
     gene            complement(8718..10073)
                     /gene="melA"
                     /locus_tag="OO357_002350"
     CDS             complement(8718..10073)
                     /gene="melA"
                     /locus_tag="OO357_002350"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HCR5887269.1"
                     /translation="MMSAPKITFIGAGSTIFVKNILGDVFHREALKTAHIALMDIDPT
                     RLEESHIVVRKLMDSAGASGKITCHTQQKEALEDADFVVVAFQIGGYEPCTVTDFEVC
                     KRHGLEQTIADTLGPGGIMRALRTIPHLWQICENMTEVCPDATMLNYVNPMAMNTWAM
                     YARYPYIKQVGLCHSVQGTAEELARDLNIDPATLRYRCAGINHMAFYLELERKTADGS
                     YVNLYPELLAAYEAGQAPKPNIHGNTRCQNIVRYEMFKKLGYFVTESSEHFAEYTPWF
                     IKLGREDLIERYKVPLDEYPKRCVEQLANWHKELEEYKNASRIDIKPSREYASTIMNA
                     IWTGEPSVIYGNVRNDGLIDNLPQGCCVEVACLVDANGIQPTKVGTLPSHLAALMQTN
                     INVQTLLTEAILTENRDRVYHAAMMDPHTAAVLGIDEIYALVDDLIAAHGDWLPSWLH
                     R"
     gene            10356..11264
                     /gene="melR"
                     /locus_tag="OO357_002351"
     CDS             10356..11264
                     /gene="melR"
                     /locus_tag="OO357_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator MelR"
                     /protein_id="HCR5887270.1"
                     /translation="MNTDTFMCSSDEKQTRSPLSLYSEYQRMEIEFRAPHIMPTSHWH
                     GQVEVNVPFDGDVEYLINNEKVNINQGHITLFWACTPHQLTDTGTCQSMAIFNLPMHL
                     FLSWPLDKDLINHVTHGMVIKSLATQQLSPFEVRRWQQELNSPNEQIRQLAIDEIGLM
                     LKRFSLSSWEPILVNKTSRTHKNSVSRHAQFYVSQMLGFIAENYDQALTINDVAEHVK
                     LNANYAMGIFQRVMQLTMKQYITAMRINHVRALLSDTDKSILDIALTAGFRSSSRFYS
                     TFGKYVGMSPQQYRKLSQQRRQTFPG"
     gene            11463..13730
                     /gene="adiA"
                     /locus_tag="OO357_002352"
     CDS             11463..13730
                     /gene="adiA"
                     /locus_tag="OO357_002352"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine decarboxylase"
                     /protein_id="HCR5887271.1"
                     /translation="MKVLIVESEFLHQDTWVGNAVERLADALSQQNVTVIKSTSFDDG
                     FAILSSNEAIDCLMFSYQMEHPDEHQNVRQLIGKLHERQQNVPVFLLGDREKALAAMD
                     RDLLELVDEFAWILEDTADFIAGRAVAAMTRYRQQLLPPLFSALMKYSDIHEYSWAAP
                     GHQGGVGFTKTPAGRFYHDYYGENLFRTDMGIERTSLGSLLDHTGAFGESEKYAARVF
                     GADRSWSVVVGTSGSNRTIMQACMTDNDVVVVDRNCHKSIEQGLMLTGAKPVYMVPSR
                     NRYGIIGPIYPQEMQPETLQKKISESPLTKDKAGQKPSYCVVTNCTYDGVCYNAKEAQ
                     DLLEKTSDRLHFDEAWYGYARFNPIYADHYAMRGEPGDHNGPTVFATHSIHKLLNALS
                     QASYIHVREGRGAINFSRFNQAYMMHATTSPLYAICASNDVAVSMMDGNSGLSLTQEV
                     IDEAVDFRQAMARLYKEFTADGSWFFKPWNKEVVTDPQTGKTYDFADAPTKLLTTVQD
                     CWVMHPGESWHGFKDIPDNWSMLDPIKVSILAPGMGEDGELEETGVPAALVTAWLGRH
                     GIVPTRTTDFQIMFLFSMGVTRGKWGTLVNTLCSFKHHYDANTPLAQVMPELVEQYPD
                     TYANMGIHDLGDTMFAWLKENNPGARLNEAYSGLPVAEITPREAYNAIVDNNVELVSI
                     ENLPGRIAANSVIPYPPGIPMLLSGENFGDKNSPQVSYLRSLQSWDHHFPRFEHETEG
                     TEIIDGIYHVMCVKA"
     gene            14055..14816
                     /gene="adiY"
                     /locus_tag="OO357_002353"
     CDS             14055..14816
                     /gene="adiY"
                     /locus_tag="OO357_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator AdiY"
                     /protein_id="HCR5887272.1"
                     /translation="MRICSDQPCIVLLTEKDVWIRVNGKEPISLKANHMALLNCENNI
                     IDVSSLNNTLVAHISHDIIKDYLRFLNKDLSQIPVWQRSATPILTLPCLTPDVFRVAA
                     QHSMMPAETESEKERTRALLFTVLSRFLDSKKFLSLMMYMLRNCVSDSVYQIIESDIH
                     KDWNLSMVASCLCLSPSLLKKKLKSENTSYSQIITTCRMRYAVNELMMDGKNISQVSQ
                     SCGYNSTSYFISVFKDFYGMTPLHYVSQHRERTVA"
     gene            14953..16290
                     /gene="adiC"
                     /locus_tag="OO357_002354"
     CDS             14953..16290
                     /gene="adiC"
                     /locus_tag="OO357_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine/agmatine antiporter"
                     /protein_id="HCR5887273.1"
                     /translation="MSSDADAHKVGLIPVTLMVSGNIMGSGVFLLPANLASTGGIAIY
                     GWLVTIIGALGLSMVYAKMSFLDPSPGGSYAYARRCFGPFLGYQTNVLYWLACWIGNI
                     AMVVIGVGYLSYFFPILKDPLVLTITCVVVLWIFVLLNIVGPKMITRVQAVATVLALI
                     PIVGIAVFGWFWFRGETYMAAWNVSGLGTFGAIQSTLNVTLWSFIGVESASVAAGVVK
                     NPKRNVPIATIGGVLIAAVCYVLSTTAIMGMIPNAALRVSASPFGDAARMALGDTAGA
                     IVSFCAAAGCLGSLGGWTLLAGQTAKAAADDGLFPPIFARVNKAGTPVAGLIIVGILM
                     TIFQLSSISPNATKEFGLVSSVSVIFTLVPYLYTCAALLLLGHGHFGKARPAYLAVTT
                     IAFLYCIWAVVGSGAKEVMWSFVTLMVITAMYALNYNRLHKNPYPLDAPISKD"
     gene            16394..18037
                     /gene="eptA"
                     /locus_tag="OO357_002355"
     CDS             16394..18037
                     /gene="eptA"
                     /locus_tag="OO357_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418538.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoethanolamine transferase EptA"
                     /protein_id="HCR5887274.1"
                     /translation="MLKRLLKRPSLNLLAWLLLAAFYISICLNIAFFKQVLQALPLDS
                     LHNVLVFLSMPVVAFSVINIVLTLSSFLWLNRPLACLFILIGAAAQYFIMTYGIVIDR
                     SMIANIIDTTPAESYALMTPQMLLTLGFSGVLAELIACWLKIKPATSRLRSVLFRGAN
                     ILVSVLLILLVAALFYKDYASLFRNNKELVKSLSPSNSIVASWSWYSHQRLANLPLVR
                     IGEDAHRNPLMQNEKRKNLTILIVGETSRAENFSLNGYPRETNPRLAKDNVVYFPNTA
                     SCGTATAVSVPCMFSDMPREHYKEELAQHQEGVLDIIQRAGINVLWNDNDGGCKGACD
                     RVPHQNVTALNLPDQCINGECYDEVLFHGLEEYINNLQGDGVIVLHTIGSHGPTYYNR
                     YPPQFRKFTPTCDTNEIQTCTKEQLVNTYDNTLVYVDYIVDKAINLLKEHQDKFTTSL
                     VYLSDHGESLGENGIYLHGLPYAIAPDSQKQVPMLLWLSEDYQKRYQVDQNCLQKQAQ
                     TQHYSQDNLFSTLLGLTGVETKYYQAADDILQTCRRVSK"
     gene            18034..18702
                     /gene="basR"
                     /locus_tag="OO357_002356"
     CDS             18034..18702
                     /gene="basR"
                     /locus_tag="OO357_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BasR"
                     /protein_id="HCR5887275.1"
                     /translation="MKILIVEDDTLLLQGLILAAQTEGYACDGVTTARMAEQSLEDGH
                     YSLVVLDLGLPDEDGLHFLARIRQKKYTLPVLILTARDTLTDKIAGLDVGADDYLVKP
                     FALEELHARIRALLRRHNNQGESELIVGNLTLNMGRRQVWMGGEELILTPKEYALLSR
                     LMLKAGSPVHREILYNDIYNWDNEPSTNTLEVHIHNLRDKVGKARIRTVRGFGYMLVA
                     NEEN"
     gene            18703..19803
                     /gene="pmrB"
                     /locus_tag="OO357_002357"
     CDS             18703..19803
                     /gene="pmrB"
                     /locus_tag="OO357_002357"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     PmrB"
                     /protein_id="HCR5887276.1"
                     /translation="MNLMRFLRRPISLRQRLILTIGAILLVFELISVFWLWHESTEQI
                     QLFEQALRDNRNNDRHIMREIREAVASLIVPGVFMVSLTLFICYQAVRRITRPLAELQ
                     KELEARTADNLTPIAIHSATLEIEAVVSALNDLVSRLTSTLDNERLFTADVAHELRTP
                     LAGVRLHLELLAKTHHIDVAPLVARLDQMMESVSQLLQLARAGQSFSSGNYQHVKLLE
                     DVILPSYDELSTMLDQRQQTLLLPESAADITVQGDATLLRMLLRNLVENAHRYSPQGS
                     NIMIKLQEDGGAVMAVEDEGPGIDESKCGELSKAFVRMDSRYGGIGLGLSIVSRITQL
                     HHGQFFLQNRQETSGTRAWVRLKKDQNVANQI"
     gene            complement(19779..19868)
                     /gene="pmrR"
                     /locus_tag="OO357_002358"
     CDS             complement(19779..19868)
                     /gene="pmrR"
                     /locus_tag="OO357_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000797656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LpxT activity modulator PmrR"
                     /protein_id="HCR5887277.1"
                     /translation="MKNRVYESLTTVFSVLVVSSFLYIWFATF"
     gene            complement(19980..21482)
                     /gene="proP"
                     /locus_tag="OO357_002359"
     CDS             complement(19980..21482)
                     /gene="proP"
                     /locus_tag="OO357_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709830.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline transporter ProP"
                     /protein_id="HCR5887278.1"
                     /translation="MLKRKKVKPITLRDVTIIDDGKLRKAITAASLGNAMEWFDFGVY
                     GFVAYALGKVFFPGADPSVQMVAALATFSVPFLIRPLGGLFFGMLGDKYGRQKILAIT
                     IVIMSISTFCIGLIPSYDTIGIWAPILLLICKMAQGFSVGGEYTGASIFVAEYSPDRK
                     RGFMGSWLDFGSIAGFVLGAGVVVLISTIVGEANFLDWGWRIPFFIALPLGIIGLYLR
                     HALEETPAFQQHVDKLEQGDREGLQDGPKVSFKEIATKYWRSLLTCIGLVIATNVTYY
                     MLLTYMPSYLSHNLHYSEDHGVLIIIAIMIGMLFVQPVMGLLSDRFGRRPFVLLGSVA
                     LFVLAIPAFILINSNVIGLIFAGLLMLAVILNCFMGVMASTLPAMFPTHIRYSALAAA
                     FNISVLVAGLTPTLAAWLVESSQNLMMPAYYLMVVAVIGLITGVTMKETANRPLKGAT
                     PAASDIQEAKEILVEHYDNIEQKIDNIDHEIADLQAKRTRLVQQHPRIDE"
     gene            complement(21746..22624)
                     /locus_tag="OO357_002360"
     CDS             complement(21746..22624)
                     /locus_tag="OO357_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418534.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjcZ-like family protein"
                     /protein_id="HCR5887279.1"
                     /translation="MTKTLLDGPGRVLESVHPRFLVDLAQGDDARHPQAHQQQFRERL
                     MQELLSRVQLQTWTNGGMLNAPLSLRLTMVEKLASMLDPGHLALTQIAQHLALLQKMD
                     HRQHSAFPELPQQIAALYEWFSARCRWKEKALTQRGLLVQAGDQSEQIFTRWRAGAYN
                     AWSLPGRCFIVLEELRWGAFGDACRLGSPQAVVLLLGDLREKATQHLAESINAAPTTR
                     HYYHQWFASSTVPTGGDHADFLSWLGKWTTADKQPVCWSVTQRWQTVALGMPRLCSAQ
                     RLVGAMVEEIFSVNLA"
     gene            complement(22621..24849)
                     /gene="crfC"
                     /locus_tag="OO357_002361"
     CDS             complement(22621..24849)
                     /gene="crfC"
                     /locus_tag="OO357_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="clamp-binding protein CrfC"
                     /protein_id="HCR5887280.1"
                     /translation="MYTQTLYELSQEAERLLQLSRQQLQLLEKMPLSVPGDDAPQLAL
                     PWSQPNIAERHAMLNNELCKISRLEMVLAIVGTMKAGKSTTINAIVGTEVLPNRNRPM
                     TALPTLIRHTPGQKEPVLHFSHVAPIDCLIQQLQQRLRDCDIKHLTDVLEIDKDMRAL
                     MQRIENGVAFEKYYLGAQPIFHCLKSLNDLVRLAKALDVDFPFSAYAAIEHIPVIEVE
                     FVHLAGLESYPGQLTLLDTPGPNEAGQPHLQKMLNQQLARASAVLAVLDYTQLKSISD
                     EEVREAILAVGQSVPLYVLVNKFDQQDRNSDDADQVRALISGTLMKGCITPQQIFPVS
                     SMWGYLANRARYELANNGKLPAPEQQRWVEDFAHAALGRRWRHADLADLEHIRHAADQ
                     LWEDSLFAQPIQALLHAAYANASLYALRSAAHKLLNYAQQAREYLDFRAHGLNVACEQ
                     LRQNIHQVEESLQLLQLNQAQVSGEIKHEIELALTSANHFLRQQQDAVNAQLAALFQD
                     DSEPLSEIRTRCETLLQTAQNTISRDFTLRFAEMESTLCRVLTDVIRPIEQQVKMELS
                     ESGFRPGFHFPVFHGVVPHFNTRQLFSEVISRQEATDEQSTRLGVVRETFSRWLNQPD
                     WGRGNEKSPTETVDYSVLQRALSAEVDLYCQQMAKVLAEQVDESVTAGMNTFFAEFAS
                     CLTELQTRLRESLALRQQNESVVRLMQQQLQQTVMTHSWIYTDAQLLRDDIQTLFTAE
                     RY"
     gene            25252..25587
                     /locus_tag="OO357_002362"
     CDS             25252..25587
                     /locus_tag="OO357_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phnA family protein"
                     /protein_id="HCR5887281.1"
                     /translation="MSLPHCPKCNSEYTYEDNGMYICPECAYEWNDAEPAQESDELIV
                     KDANGNLLADGDSVTIIKDLKVKGSSSMLKIGTKVKNIRLVEGDHNIDCKIDGFGPMK
                     LKSEFVKKN"
     gene            25745..26188
                     /gene="yjdN"
                     /locus_tag="OO357_002363"
     CDS             25745..26188
                     /gene="yjdN"
                     /locus_tag="OO357_002363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family metalloprotein YjdN"
                     /protein_id="HCR5887282.1"
                     /translation="MPLSPYLSFAGNCSDAIAYYQRTLGAELLYKISFGEMPKSAQDS
                     AENCPSGMQFPDTAIAHANVRIAGSDIMMSDAIPSGKASYSGFTLVLDSQQVEEGKRW
                     FDNLAANGKIEMAWQETFWAHGFGKVTDKFGVPWMINVVKQQPTQ"
     gene            26321..27109
                     /gene="phnC"
                     /locus_tag="OO357_002364"
     CDS             26321..27109
                     /gene="phnC"
                     /locus_tag="OO357_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HCR5887283.1"
                     /translation="MQTIIRVEKLAKTFNQHQALHAVDLNIHHSEMVALLGPSGSGKS
                     TLLRHLSGLITGDKSAGSHIELLGRTVQREGRLARDIRKSRANTGYIFQQFNLVNRLS
                     VLENVLIGALGSTPFWRTCFSWFTGEQKQRALQALTRVGMVHFAHQRVSTLSGGQQQR
                     VAIARALMQQAKVILADEPIASLDPESARIVMDTLRDINQNDGITVVVTLHQVDYALR
                     YCERIVALRQGHVFYDGSSQLFDNERFDHLYRSINRIEENAKAA"
     gene            27134..28150
                     /gene="phnD"
                     /locus_tag="OO357_002365"
     CDS             27134..28150
                     /gene="phnD"
                     /locus_tag="OO357_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5887284.1"
                     /translation="MNAKIIASLAFTSMFSLSTLLTPAHAEEQEKALNFGIISTESQQ
                     NLKPQWTPFLQDMEKKLGVKVNAFFAPDYAGIIQGMRFNKVDIAWYGNLSAMEAVDRA
                     NGQVFAQTVAADGSPGYWSVLIVNKDSPINNLNDLLAKRKDLTFGNGDPNSTSGFLVP
                     GYYVFAKNNISASDFKRTVNAGHETNALAVANKQVDVATNNTENLDKLKTSAPEKLKE
                     LKVIWKSPLILGDPIVWRKNLSETTKDKIYDFFMNYGKTPEEKAVLERLGWAPFRASS
                     DLQLVPIRQLALFKEIQSVKGNKGLNEQDKLAKTTEIQAQLDDLDRLNNALSAMSSVS
                     KAVQ"
     gene            28256..>28404
                     /locus_tag="OO357_002366"
     CDS             28256..>28404
                     /locus_tag="OO357_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313113.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane channel protein of phosphonate
                     transporter"
                     /protein_id="HCR5887285.1"
                     /translation="MQTITIAPPKRSWFSLLSWAVVLDVLVVSWQGAEMAPLTLIKDG
                     GNMAT"
BASE COUNT         7296 a         7417 c         6935 g         6756 t
ORIGIN      
        1 gactttcgtt tgagattaaa taacttgccg ttgccacgta cgcaattgcc agaatgctga
       61 catatcgcag tgttgaaata gacatcgatc ttttcgccgg tataacagcg ataacccccg
      121 tccagtagcg cctgatccat gaccttccct ctctcatcag tatgattatc gctataagca
      181 tagccccgga atcttcatac agcactggca gtttccgttg ccagagtctc ttcccctata
      241 tagccaatca tttttactcc ctttgaatta cccgcctcat cagagataat gcttaagaaa
      301 tttgtcacac aaggaagctg atgagacatt cattgcccta ccacatatta cgcaaacgtc
      361 cgatgaaatt gagtaccact gtgatcttaa tggtcagcgc ggtactgttc tcggtgctat
      421 tggtggtgca tctgatttac ttctcgcaaa tcagtgatat gacgcgagac gggctggcca
      481 acaaggcact ggcagtggcg cgtaccctcg ccgactcgcc ggaaatccgt cagggcttgc
      541 agaaaaaacc gcaggagagt ggcatccagg ccatcgcgga agccgtgcgc aaacgcaacg
      601 atctgctgtt tattgtcgtt accgatatgc aaagtcttcg ctactcacat cctgaagccc
      661 agcgtattgg tcagccattt aaaggtgatg acatccttaa tgcgctgaat ggcgaagaaa
      721 atgtcgctat caatcgcggt tttctggcgc aggctttacg cgtatttacc cccatctacg
      781 atgaaaatca taaacaaatt ggcgtggtgg cgatcggcct tgagttaagc cgcgtgaccc
      841 aacagatcaa tgacagtcgc tggagcatta tctggtcggt attatttggc atgctggtcg
      901 gactgattgg cacctgcatt ctggttaagg tactgaaaaa aatccttttc ggcctggaac
      961 cctacgaaat ctccacgctg tttgagcaac gccaggccat gttgcagtct atcaaagaag
     1021 gcgtcgttgc cgtggacgat cgcggcgagg tcacgctgat caacgatgcc gcacaagaat
     1081 tgctgaatta ccgtaagtcg caggacgatg agaaactgtc gacgctcagc cactcatggt
     1141 cacaggtggt agatgtctcg gaagtgttac gcgacggtac gccgcgccgc gacgaagaga
     1201 ttacgattaa agaccggcta ttactgatca acaccgttcc ggtgcgcagt aatggcgtta
     1261 tcatcggtgc catttcaacc ttcagggaca aaactgaagt tcgtaaactg atgcagcgac
     1321 tcgacggtct ggtcaactat gctgacgcac ttcgtgaacg atcccacgaa tttatgaata
     1381 aattgcatgt gattctcgga ttattgcatc tgaagagtta taagcagttg gaagattaca
     1441 ttctcaaaac agccaataac tatcaggaag agattggctc tctgctgggt aagatcaaat
     1501 ctccggttat cgctggtttt ttaatcagca agattaaccg cgcgaccgat ttaggccata
     1561 cgctgatttt aaacagtgaa agccagctgc cagacagcgg cagtgaggac caggtcgcga
     1621 cgctgattac cacattggga aatctgatag aaaacgcgct ggaagcatta gggccggaac
     1681 ccggtggcga aattagcgta acattgcact accgtcacgg ctggctgcac tgcgaagtta
     1741 acgatgatgg accggggatc gcacccgaca aaatcgatca catttttgac aaaggtgtct
     1801 cgacaaaagg aagcgagcga ggcgtcggtt tagcacttgt caaacaacag gtagaaaatc
     1861 tcggcggcag catcgccgtg gaatcggaac cggggatttt cacacaattt tttgtccaga
     1921 taccctggga cggggagagg tcgaacagat gatcaatgta ttaattatcg atgacgacgc
     1981 aatggtcgcg gagctgaatc gccgatacgt agcacaaatc ccaggctttc aatgctgtgg
     2041 aacagcctcg acgctggaga aagccaaaga gattatcttc aatagcgatg cgcctatcga
     2101 cctgatattg ctcgatatct atatgcaaaa agagaacggg ctcgatttac tgcctgtcct
     2161 gcataacgcg cgttgcaaaa gtgatgtgat tgtcatctcc tccgcagccg atgcggcaac
     2221 cattaaagat tcgctgcatt acggtgtcgt ggattacctg atcaaacctt tccaggcttc
     2281 ccgctttgaa gaggcgctca ccggctggcg gcaaaagaaa atggcgctgg aaaaacatca
     2341 gtattatgac caggccgagc ttgatcagct aatccacggc agcagctcca acgaacagga
     2401 tcctcgtcgc ttgccaaaag gcttaacgcc gcagacgtta cgcacgctgt gccagtggat
     2461 tgacgcgcat caggactatg agttttcaac cgacgagctg gctaacgagg ttaacatctc
     2521 gcgtgtttcc tgccgaaaat acctcatctg gctggtcaac tgccacatct tgttcaccag
     2581 tatccattat ggcgtcacgg ggcgtccggt ttaccgttac cgtattcagg cagaacacta
     2641 ctcactgctg aaacaatatt gccaataacg gatcgctggt tatctgtaag taataattat
     2701 taccgtaatg caaaataccc ctggcacaag ttacatcgtt aatcagtaaa acttgtgcca
     2761 gatcaaatat aattatccct ccatcatcgt taaaaattaa tatctcttca gatgaacggt
     2821 gtttttaatt tcaaaacgat aacaaaagtt aattaactat tatgtcaccc gcattatgtg
     2881 tatttttacc cacaaatggg tagatcagat taatctataa acctaatgat atctgccctg
     2941 agaacaaaaa atagaccgat aaatatcaat aagataacag caaacaaaac attaacatct
     3001 gcgcagtaca aactataaac ccatcgccag agagtttttc tctctgaaaa agccgcttat
     3061 cacagtgcat aaatttgccg ctgctttaat cagccaatat tcactgtgag gtatttgcta
     3121 aagccggtaa cgaccaaacg gatatttagt caggctctga aaacagttca tacaaaacag
     3181 aacgtgactg tgatctattc agcaaaaatt taaataggat tatcgcgagg gttcacacat
     3241 gttatttact atccaactta tcataatact aatatgtctg ttttatggtg ccagaaaggg
     3301 tggtatcgcg ctgggtttat taggcggtat cggtctggtc attctggtct tcgtcttcca
     3361 ccttcagcca ggtaaaccac cagttgatgt catgctggtt atcattgcgg tggtggcggc
     3421 atcggcgacc ttgcaagctt cgggcggtct tgatgtcatg ctgcaaattg ccgagaagct
     3481 gctgcgccgc aacccgaaat atgtctcaat tgtcgcgccg tttgtgacct gtacactgac
     3541 cattctttgc ggtacgggtc atgtggttta caccattctg ccgatcatct acgacgtcgc
     3601 cattaagaac aacatccgtc cggaacgtcc gatggcggca agttctatcg gtgcacagat
     3661 ggggattatc gccagtccgg tgtcggttgc ggtcgtgtct ctggttgcga tgctgggtaa
     3721 tgtcaccttt gatggtcgcc atcttgagtt cctcgatctg ctggcaatca ccattccatc
     3781 gacgttaatc ggtatcctgg cgatcggtat cttcagctgg ttccgcggta aagatctgga
     3841 taaagacgaa gagttccaga aattcatctc cgtaccggaa aaccgtgagt atgtttacgg
     3901 tgataccgcg acgctgctgg ataaaaaact gccgaaaagc aactggctgg caatgtggat
     3961 tttcctcggg gcaatcgctg tagtcgccct tcttggtgct gattcggacc tgcgtccatc
     4021 cttcggcggc aaaccgctgt cgatggtact ggttattcag atgtttatgc tgctgaccgg
     4081 ggcgctgatt attatcctga ccaaaaccaa tcccgcgtct atctcaaaaa acgaagtctt
     4141 ccgttccggt atgatcgcca tcgtggcggt gtacggtatc gcatggatgg cagaaaccat
     4201 gttcggtgcg catatgtctg aaattcaggg cgtactgggt gaaatggtga aagagtatcc
     4261 gtgggcgtat gccattgttc tgctgctggt ttccaagttt gtaaactctc aggctgcggc
     4321 gctggcggcg attgttccgg tcgcgctggc gatcggcgtt gatccggcat acatcgtggc
     4381 ttcagcaccg gcttgctacg gttattacat cctgccgact tatccgagcg atctggcagc
     4441 gattcagttt gaccgttccg gcaccaccca catcggtcgc ttcgtcatca accacagttt
     4501 tattctgccg gggttgattg gtgtgagcgt atcgtgcgtc ttcggctgga tcttcgccgc
     4561 gatgtacggg ttcttataaa tgcactttgc gtgccgcccg tgactacgcg gcacgccatt
     4621 ttcgaataac aaatacagag ttacaggctg gaagctatgt caaacaaacc ctttatctac
     4681 caggcacctt tcccgatggg gaaagacaat accgaatact atctactcac ttccgattac
     4741 gttagcgttg ccgacttcga cggcgaaacc atcctgaaag tggaaccaga agccctgacc
     4801 ctgctggcgc agcaagcctt tcacgacgct tctttcatgc tacgtccggc acaccagaaa
     4861 caggttgcgg ctattcttca cgatccagaa gccagcgaaa acgacaagta cgtggcactg
     4921 caattcttaa gaaactccga aatcgccgcc aaaggcgtgc tgccgacctg ccaggatacc
     4981 ggcaccgcga tcatcgtcgg taaaaaaggc cagcgcgtgt ggaccggtgg cggtgatgaa
     5041 gaagcgctgt cgaaaggcgt ctataacacc tatatcgaag ataacctgcg ctactcgcaa
     5101 aacgcggcgc tggacatgta caaagaggtc aacaccggca ctaacctgcc tgcgcaaatc
     5161 gacctgtacg cggtagatgg cgatgagtac aaattccttt gcgttgcgaa aggcggcggc
     5221 tctgccaaca aaacgtatct ctaccaggaa accaaagccc tgctgactcc cggcaaactg
     5281 aaaaacttcc tcgtcgagaa aatgcgtacc ctcggtactg cagcctgccc gccgtaccat
     5341 atcgcgtttg tgattggcgg tacgtctgcg gaaaacaacc tgaaaaccgt caaattagca
     5401 agcgctcact attacgatga actgccgacg gaagggaacg aacatggtca ggcgttccgc
     5461 gatgtccagc tggaacagga actgctggaa gaggcccaga aactcggtct tggcgcgcag
     5521 tttggcggta aatacttcgc gcacgacatt cgcgttatcc gtctgccacg tcacggcgca
     5581 tcctgcccgg taggcatggg cgtctcctgc tccgctgacc gtaacattaa agcgaaaatc
     5641 aaccgcgaag gtatctggat cgaaaaactg gaacacaatc taggtcagta cattccacaa
     5701 gaactgcgtc aggccggtga aggcgaagcg gtgaaggtcg accttaaccg cccgatgaaa
     5761 gagatcctcg cccagctttc gcaatatccg gtttccactc gtttgtcgct cactggcacc
     5821 attattgtgg gtcgagatat tgcacacgcc aagctgaaag agctgattga cgccggtaaa
     5881 gaacttccgc agtacatcaa agatcacccg atctactacg cgggtccggc gaaaacccct
     5941 gccggttatc catcaggttc acttggccca accaccgcag gtcgtatgga ctcctatgtg
     6001 gatctgctgc aatcccacgg cggcagcatg atcatgctgg cgaaaggcaa ccgcagccag
     6061 caggttaccg acgcgtgtca taaacacggc ggcttctacc tcggtagcat cggcggtccg
     6121 gcggcggtac tggcgcagca gagcatcaag catctggaat gcgtcgctta tccggagctg
     6181 ggtatggaag ctatctggaa aatcgaagta gaagatttcc cggcgtttat cctggtcgat
     6241 gacaaaggta acgacttctt ccagcaaatc gtcaacaaac agtgcgcgaa ctgcactaag
     6301 taacctcttc ggcccagcgc ctggcagcat gctgccaggt gatccccctg ggccacctct
     6361 tttgcgattg taatttcatg cttgctggtg aatagtcagt attttccctg acttgcgaac
     6421 tcaccatgac ccgcacactc aagccgttaa ttcttaacac tggcgcactg gcgctaacgt
     6481 taatcctgat ttataccggc atttcggccc atgacaaact cacctggctg ctggaagtga
     6541 caccggtgat tatcgtcgtg ccgctactac ttgccaccgc cagacgttac ccgttaacgc
     6601 tgctgctcta tacgctcatc ttctttcacg ccatcatcct gatggtaggc gggcagtaca
     6661 cctacgcgaa agtccccgtt ggttttgagg tgcaggaatg gctggggttg agtcgtaatc
     6721 cgtatgacaa gctggggcac tttttccagg ggctggtgcc tgcactggtg gcacgagaaa
     6781 ttctcgttcg cgggatgtac gtgcgcggac gtaaaatggt ggcgtttctg gtctgttgcg
     6841 tggcgctggc gataagcgcg atgtatgaat taatcgagtg gtgggcggca ctggcgatgg
     6901 gccagggagc ggatgatttt ctcggtaccc agggcgacca gtgggacacg caatccgata
     6961 tgttctgcgc gctgcttggc gcattaacga cagtgatatt gctcgctcgc tttcattgcc
     7021 gccagctacg gcgctatggc ttgataactg gataacaacg tgcactttgt tgatgccgga
     7081 tgcggcgtga attccttatc cggtctacaa aatttgcaaa tccaaagcat tacattaaga
     7141 cgtggcctga taaacatatc gcatcaggcc attttcgtta gctattgtcc acgtcaggct
     7201 ttcacatcac tcactgcacc gaccggaata tcagcatgaa caggctcggg cggtactttg
     7261 cgatatttat ccagcagatg aatctggatc ctgcgcagcg tgtcgccatt gaggcgatag
     7321 aaacggaagt agagaatcag cgttaccatg aaaaacagag tcggtagagc aatcataata
     7381 aactgcatac ctaatagggc ttgcgtagac tgttcaacat tcggtacata gccaatcatc
     7441 cctaacacaa ccgcaatgaa aaaagccgca aaggctgaac cgcccttcac caccatagtc
     7501 tgcacggagt aagcgatgct ttcacagcgt acgtgcagtt tatattcacc gtaatcaacg
     7561 gtatctgcca ccatgatgac ctgtaatacc cagaaaagcg ccgttcccac attcagcaaa
     7621 atacccgcaa tcacaatgag gacgacgttg tgatagctca ttaatgccat taacaggaga
     7681 acaccacagc ttaacaccgg aagaatagat gctccggccc ataaaatgcg tcgggataat
     7741 gatttaacta agcgtgggaa gaatactaac gtcaccaggt tagcagctcc cgcatacgac
     7801 agataatagg ggaacaaatc cgcatcaccg ataacatatg agaaataata gatagcaaat
     7861 ccggtaataa tgttgctggc tacattataa gcaagagcca tacccaagag gcatgaaagc
     7921 tgatcgtttt tataaataag cgcaacgatg gctttaagtg tcagatggct tccttcagca
     7981 gacgattgat tgtctgacga aaagacttca tgcacattgc gcagagtgat gatggttgaa
     8041 acaataaaaa aggcgatcaa taccagagtg aacatctgaa agccaaatcc ccgatcgcca
     8101 ccgccgacat aattaacaaa tggtagcgtc acacctgccg taacaaagcc tgccagactg
     8161 gcaaaaaaac gcggataagg aaccagttgt tcgcgttcac gtttatcgag cgtgatggtt
     8221 ggaaccagcg accagaaggg aatatccata atggtgtaag tcatgcccca gaggatgtag
     8281 gtcacgcaaa caaagacaat ctgagtagta ccttcaaaca gatgcgcact aaagaggaga
     8341 aataagatta cagagtttgc caacgtaccg atcaggatcc agggtttgaa cttaccccat
     8401 cgcgatcgcg tagcatttac aatccatccc ataatcggat cgttaatagc atcccagatc
     8461 ctcgccacca gaaacaaagt accgaccaaa cccacagaca gcccgacgac atcggtgtag
     8521 taatacatga ggtacatata cacaatgccg atcgcaaaat ccttcccgaa cgctccaaat
     8581 ccataactga gttttgtagt cattgaaatg ctcatagggt atcgggtcgc tgttaccaac
     8641 gcccgccttc tgttattggt atcgtcagag gtgtgcgccg gaataaatcc cccggcgcag
     8701 tagcgtttag tcgcgtttta acggtgcaac cagcttggca gccagtcgcc gtgggcggca
     8761 atcaggtcgt caacaagagc atatatttcg tcaatgccca gcacggcggc agtatgcggg
     8821 tccatcatcg cggcgtggta aacacggtcg cgattttccg taagaatagc ttcggtcagc
     8881 agcgtctgta cgttgatgtt ggtttgcatc agggcggcca gatgcgaagg tagcgtaccg
     8941 actttggtcg gctgaatgcc attagcatca accagacagg ctacttccac gcaacatcct
     9001 tgtggcaggt tatcaatcaa accatcgtta cggacgttgc cgtaaatcac actcggctcg
     9061 ccagtccaga tagcgttcat gattgtgctg gcatattccc gtgacggttt aatatcaatc
     9121 cgggaggcgt ttttatactc ctccagctct ttatgccagt tcgccagctg ctcgacgcag
     9181 cgtttcgggt actcatccag cggtacttta taacgctcaa tcaaatcctc acgacctagc
     9241 ttaataaacc acggcgtata ttcggcaaaa tgttctgatg attccgtgac gaagtagccc
     9301 agctttttga acatttcgta gcgcacaata ttctggcagc gagtattacc atgaatattt
     9361 ggcttcggtg cctgccctgc ttcataagcc gccagcagtt ccgggtagag attcacgtaa
     9421 ctgccgtcgg cggttttgcg ctccagctcc aggtaaaacg ccatatggtt gatacctgcg
     9481 caacggtaac gcagcgtagc cgggtcgata ttgaggtcac gcgccagctc ttccgccgtt
     9541 ccctgcaccg aatggcacag cccgacctgt ttgatatacg gatagcgggc atacatcgcc
     9601 caggtattca tcgccattgg gttaacatag ttgagcatgg tggcatcggg gcagacttcc
     9661 gtcatgttct cgcaaatttg ccacagatgc ggaatggtac gtagcgcgcg cataataccg
     9721 cccggcccca acgtatcggc aatggtttgt tccagaccat gccgcttaca gacctcgaaa
     9781 tcagtcaccg tgcaaggttc ataaccgcca atctgaaatg ccaccacgac aaaatcggca
     9841 tcctctaagg cttctttctg ttgggtgtgg caggtgattt tgccgctggc ccctgctgaa
     9901 tccatcagct tacgcaccac aatatgcgac tcttccaggc gggtggggtc aatgtccatc
     9961 agggcaatat gcgccgtttt cagcgcctcg cgatggaaca catcaccaag aatattttta
    10021 acgaaaatcg tcgaaccagc gccgataaat gtaattttgg gtgcagacat catgcagatc
    10081 tcctggcttg cttgaataac ttcatcatgg caggcgaatc tgcagaaaac ctccggcttc
    10141 caggttgagc attcccataa actcagatcc tgcgtgaagc agcagtaaat ctgagtttat
    10201 gggaataaat tacctgaaaa gtagagtttt cggcctcatg atgctcggac agtcccctcg
    10261 cccctctggg aagagggtga aaaccgtgct cccactcgca gtcatcctcc ctcactcctg
    10321 ccataattct gatattctag gaaagagagc catccatgaa tacagatacg tttatgtgca
    10381 gcagcgacga aaaacagacc cgcagcccgc tgtcgctgta ttcggaatac cagcggatgg
    10441 agattgagtt tcgcgcaccg catatcatgc ccaccagcca ctggcatggt caggtcgaag
    10501 tgaatgtgcc tttcgatggc gatgtggaat acctgatcaa caatgaaaaa gtgaatatca
    10561 atcagggtca tatcacgctg ttctgggcct gtacaccgca ccaactaaca gataccggaa
    10621 cctgtcagag catggcgatt tttaatctgc cgatgcatct gtttctctcc tggccgctgg
    10681 ataaagacct gattaaccac gtcactcacg gcatggtgat caaatcactt gccacacagc
    10741 aacttagccc gtttgaagta cgccgctggc agcaggaatt aaacagtccg aatgagcaaa
    10801 ttcgccagct cgccattgat gaaattggcc tgatgctcaa gcgatttagc ctctctagct
    10861 gggaaccgat tctggtcaat aaaacctcgc gcacacacaa aaacagcgtc tcgcgccatg
    10921 cgcaatttta tgttagccag atgctgggct ttattgccga aaactatgat caggcgctga
    10981 ccatcaacga tgtggctgag cacgtcaaac ttaacgccaa ctatgcaatg gggatattcc
    11041 agcgggtcat gcaattgacg atgaaacagt acattaccgc gatgcgcatc aaccacgttc
    11101 gcgcgttact gagcgatacc gataaaagta ttctcgatat tgccctgacg gcaggctttc
    11161 gttcaagtag tcgtttttac agcacgttcg gcaaatatgt cggcatgtcg ccgcaacaat
    11221 accgcaaact tagccaacag cgccgccaga cgtttcccgg ctaaagacga tatcagtatc
    11281 agccaaaaaa atagtttagc cgtcgataat aagcagtgaa aataatttaa aataatcaca
    11341 taacgtattg tttataaaac taaatatttc aaaaatgttt gtttttcacg cgctttacag
    11401 cccgaaaagg ccggaagata cttgcccgca acgaagattc cttcataacc gggtaagcaa
    11461 tgatgaaagt attaattgtt gaaagcgagt ttctccatca agacacctgg gtcggtaacg
    11521 ccgttgagcg tctggcagat gctttaagcc agcaaaatgt taccgtgatt aaatccacct
    11581 cctttgatga tggttttgcc attctctctt caaacgaagc cattgactgc ctgatgttca
    11641 gctatcaaat ggagcatccg gacgaacatc aaaacgtcag acaattgatc ggtaagcttc
    11701 atgagcgcca acaaaacgtg ccggtcttcc tgttgggcga tcgggaaaaa gccctcgccg
    11761 caatggaccg ggatctgctg gagcttgtcg atgaattcgc ctggattctg gaagataccg
    11821 ccgactttat cgccggacgc gccgttgccg cgatgacccg ctaccgccag cagctgttgc
    11881 cgccactgtt cagcgcgctg atgaaatata gtgacatcca tgaatattcc tgggcagcgc
    11941 caggccacca gggcggcgtt ggttttacca aaacacccgc cggacgtttc taccatgact
    12001 actatggtga aaatctgttc cgtaccgaca tgggcatcga acgaacttcc ctcggttctt
    12061 tgcttgacca tactggcgca tttggcgaaa gcgaaaaata tgccgcacgc gtatttggtg
    12121 ccgatcgctc ctggtcggta gtcgtcggta cttccggctc taaccgcacc atcatgcagg
    12181 cttgcatgac cgataacgat gtcgtggtcg ttgaccgtaa ctgccataaa tccatcgaac
    12241 aaggtttgat gctgacaggc gcgaaaccgg tctatatggt gccaagccgc aaccgctacg
    12301 gcattatcgg gccaatctat ccgcaggaaa tgcaacctga aaccttgcaa aagaaaatca
    12361 gtgaaagccc gctgaccaaa gacaaagccg ggcaaaaacc gtcttactgc gtggtaacca
    12421 actgcaccta tgacggcgtg tgttataacg ctaaagaagc gcaggatctg ctggaaaaaa
    12481 cctccgatcg tctgcacttt gacgaagcct ggtacggcta tgcacgtttc aacccgatct
    12541 atgccgatca ctatgccatg cgcggcgaac ctggcgatca caacggtcct accgttttcg
    12601 ccacccactc catccacaaa ctgctgaatg cgctgtcaca ggcttcttat attcatgtac
    12661 gtgaaggtcg tggggcgatt aacttctccc gcttcaacca ggcctacatg atgcatgcca
    12721 ccacctcccc gctgtatgcc atctgcgcat ccaacgacgt ggcggtgtcg atgatggacg
    12781 gcaacagcgg cctgtcactg acacaggaag tgattgacga agcggttgat ttccgtcagg
    12841 cgatggcgcg gctatataaa gagttcaccg ctgacggtag ctggttcttc aaaccgtgga
    12901 acaaagaagt cgtcaccgac ccacaaaccg gcaaaaccta tgactttgct gacgcaccaa
    12961 ccaaactgct gaccaccgtt caggactgct gggtaatgca tccgggcgaa agctggcacg
    13021 gcttcaaaga tattccggat aactggagta tgctcgaccc aattaaagtc agcatccttg
    13081 ctccgggaat gggtgaagat ggtgaactgg aagaaaccgg tgttccggcg gcgctggtca
    13141 ctgcctggct tggtcgccac ggcattgtgc ctacccgcac cactgacttc caaattatgt
    13201 tcctgttctc tatgggcgta acccgtggga aatgggggac tctggttaac accctttgct
    13261 ccttcaaaca ccactatgac gccaacacac cgctggcgca ggtgatgccg gaacttgttg
    13321 aacaatatcc tgacacttac gcgaacatgg ggattcacga tctgggtgac accatgtttg
    13381 cctggctgaa agaaaacaac cctggcgcac ggttgaacga agcctattcc ggcctgccgg
    13441 tggcggaaat caccccgcgt gaagcgtaca acgcgattgt cgacaacaat gtcgaactgg
    13501 tatccattga aaatctgcca ggacgcatcg cggcaaactc agttatcccg tatccgccag
    13561 gaatcccgat gctgctgtct ggtgaaaact tcggcgataa aaacagtccg caagtaagtt
    13621 atttacgctc gctgcaatcc tgggaccacc atttccctag atttgaacac gaaactgaag
    13681 ggactgaaat tattgacggt atttaccacg ttatgtgcgt gaaagcgtaa ccactattcc
    13741 gctgaaggcg taattgttta aataacatta cgccgcctgg ccttaggcct tttgagtatg
    13801 gcaacgtttt cataaaaatt gctgcaaaca aaaatgtcat actttttgcg cggccccacc
    13861 ccacgctttt ttttgcctgt tatttatcct gtaaaaaaat atgtacatga gaaattacta
    13921 taaaaatttg taatattagt aaaactcgtt atttttatgc atgtttatat tcatcatgca
    13981 attatataac catttcccgg tatcgctttg ctttagcgag aactggtctt ttattcgcac
    14041 tcaggagtac atgtatgagg atttgcagcg accaaccttg tattgtttta ttgactgaaa
    14101 aagatgtctg gataagggtg aatgggaaag aacctattag ccttaaagct aaccatatgg
    14161 cgttattaaa ttgtgaaaat aatattatcg acgtctcctc tcttaacaac actttggttg
    14221 ctcatattag tcacgacatc atcaaagatt acctccggtt tctgaataaa gatctctcgc
    14281 aaataccagt atggcaacgt agcgctacgc ccatactcac cctgccatgc ctgacgccag
    14341 acgtctttcg cgttgccgcg caacacagca tgatgcccgc agaaactgag tcagaaaagg
    14401 aacgaacacg cgcattatta ttcacggtgc tatcccgttt tctcgacagt aaaaaattcc
    14461 tttcattaat gatgtatatg ttacgtaatt gtgtaagtga cagcgtttat caaattattg
    14521 aaagcgatat tcacaaagac tggaatctta gtatggtagc cagttgttta tgtcttagcc
    14581 caagtctgtt aaagaaaaag ttgaaaagcg aaaacaccag ttatagccaa ataatcacca
    14641 cctgccgcat gcgttatgcc gtaaatgaat taatgatgga cggtaaaaat atctctcagg
    14701 tatcacagtc ctgcggctac aacagtacgt cgtactttat ttctgtcttt aaagacttct
    14761 acggtatgac gccgctgcat tatgttagtc agcacagaga acgcactgtc gcctgatttt
    14821 taaccttaac gaagagctat attaataacg gcatcagcga taacccggtc gataataatt
    14881 caactatcga atgcaggcgt atgatatgac gtaatttatt gtcacgaagc tcgccttcgc
    14941 aggagtttaa ttatgtcttc ggatgctgat gctcacaaag tgggcttaat ccccgtcacc
    15001 ctgatggtgt cggggaatat tatggggtca ggtgtttttc tgttacctgc aaacctggcc
    15061 tccactggcg ggattgccat ttatggatgg ttggtgacga ttatcggtgc gctggggctc
    15121 tcgatggtat acgccaaaat gtcgttcctc gacccaagtc ctggtggttc ttacgcttac
    15181 gcccgccgct gctttggccc gtttctcggt tatcaaacca acgtcctcta ctggctggcc
    15241 tgctggatcg gcaatatcgc catggtggtc attggcgtag gatatttaag ttacttcttc
    15301 ccgattctga aagatccatt ggtattaacc atcacctgcg tcgtggtgct gtggatcttc
    15361 gtcctgctga acattgtcgg tccgaaaatg atcacccgcg tgcaggccgt tgccactgtg
    15421 ctggcgctga tccccatcgt cgggattgcc gtatttggct ggttctggtt ccgtggtgaa
    15481 acctatatgg cggcatggaa cgtcagcggc ctgggcacct tcggtgcgat tcaaagtacc
    15541 cttaacgtta cgctgtggtc gttcatcggt gtggaaagtg cctccgttgc cgcaggtgtg
    15601 gtgaaaaacc cgaaacgcaa tgtccctatc gccaccattg gtggggtatt gattgccgcc
    15661 gtttgctatg tgctctccac caccgcgatt atggggatga tccctaatgc cgcactgcgc
    15721 gtttctgcct cgccattcgg tgatgccgca cggatggcgt tgggtgacac cgctggggcc
    15781 attgtttcct tctgcgcagc tgcgggttgc ttaggttcac tgggcggctg gacgttgctg
    15841 gcgggtcaaa cggcgaaagc cgctgccgat gacggactgt tcccaccgat ttttgcccgt
    15901 gtaaataaag cgggtacgcc agtggcgggg ctgattatcg tcggtatttt gatgaccatc
    15961 ttccagctca gcagcatttc accaaacgcg accaaagagt tcggtctggt ttcttccgtc
    16021 tcggtcatct ttacactggt gccatatctt tacacctgtg cggcgttact gctgctcggc
    16081 cacggtcact ttggtaaagc acgcccggca tatctggctg ttactaccat tgccttcctc
    16141 tactgcattt gggccgtggt ggggtccggg gcgaaagagg ttatgtggtc atttgtcacc
    16201 ctgatggtca tcaccgccat gtatgcgctg aattacaacc ggctacataa aaacccgtat
    16261 cccttagatg caccaataag caaagattaa ttccccttaa tccagcaaac ataaaagcca
    16321 accttaagaa cttaaggttg gcttaatttt gctttgcgag catatgcgca ctttgttcga
    16381 tggaaacacc gtgatgttga agcgcctact aaaaagaccc tctttgaatt tactcgcctg
    16441 gctattgttg gccgcttttt atatctctat ctgcctgaat attgcctttt ttaaacaggt
    16501 gttgcaggcg ctgccgctgg attcgctgca taacgtactg gttttcttgt cgatgccggt
    16561 tgtcgctttc agcgtgatta atattgtcct gacactaagc tctttcttat ggcttaatcg
    16621 accactggcc tgcctgttta ttctgattgg cgcggctgca caatatttca taatgactta
    16681 cggcatcgtc atcgaccgct cgatgattgc caatattatt gataccactc cagcagaaag
    16741 ttatgcgctg atgacaccgc aaatgttatt aacgctggga ttcagcggcg tgcttgctga
    16801 gctgattgcc tgctggctaa aaatcaaacc tgccacctcg cgtctgcgca gtgttctttt
    16861 ccgtggagcc aatattctgg tttctgtact gctgattttg ctggtcgccg cactgtttta
    16921 taaagactac gcctcgttgt tccgcaataa caaagagctg gtgaaatcct taagcccctc
    16981 taacagcatt gttgccagct ggtcatggta ctcccatcag cgactggcaa atctgccgct
    17041 ggtgcgaatt ggtgaagacg cgcaccgcaa cccgttaatg cagaacgaaa aacgtaaaaa
    17101 tttgaccatc ctgattgtcg gcgaaacctc gcgggcggag aacttctccc tcaacggcta
    17161 cccgcgtgaa actaacccgc ggctggcgaa agataacgtg gtctatttcc ctaataccgc
    17221 atcttgcggc acggcaacgg cagtttcagt accgtgcatg ttctcggata tgccgcgtga
    17281 gcactacaaa gaagagctgg cacagcacca ggaaggcgtg ctggatatca ttcagcgagc
    17341 gggcatcaac gtgctgtgga atgacaacga tggcggctgt aaaggtgcct gcgaccgcgt
    17401 gcctcaccag aacgtcaccg cgctgaatct gcctgatcag tgcatcaacg gcgaatgcta
    17461 tgacgaagtg ctgttccacg ggctggaaga gtacatcaat aacctgcagg gtgatggcgt
    17521 gattgtctta cacaccatcg gcagccacgg tccgacctat tacaaccgct atccgccgca
    17581 gttcaggaaa tttaccccaa cctgcgacac caatgagatc cagacctgta ccaaagagca
    17641 actggtgaac acttacgaca acacgctggt ttacgtcgac tatattgttg ataaagcgat
    17701 taatctgctt aaagaacatc aggataaatt taccaccagc ctggtttatc tttctgacca
    17761 cggtgaatcg ttaggtgaaa atggcatcta tctgcacggt ctgccttatg ccatcgcccc
    17821 ggatagccaa aaacaggtgc caatgctgct gtggctgtcg gaggattatc aaaagcggta
    17881 tcaggttgac cagaactgcc tgcaaaaaca ggcgcaaacg caacactatt cacaagacaa
    17941 tttattctcc acgctattgg gattaactgg cgttgagacg aagtattacc aggccgcgga
    18001 tgatattctg caaacttgca ggagagtgag taaatgaaaa ttctgattgt tgaagacgat
    18061 acgctgttat tgcagggact gattctggcg gcgcaaaccg aaggctacgc gtgcgatggc
    18121 gtgacaaccg cgcggatggc ggaacaaagc cttgaggacg gtcattacag cctggtggta
    18181 ctggatttag ggttacccga cgaagatgga ctgcattttc tcgcccgtat ccggcagaaa
    18241 aaatacaccc tgccggtact gatcctcacc gctcgcgata cgctgaccga caaaatcgcc
    18301 gggctggatg tcggtgccga tgactatctg gtgaagcctt ttgcgctgga agagttacac
    18361 gcccgtatcc gcgccctgct acgacgccat aataatcagg gcgaaagtga gctgattgtt
    18421 ggcaatctga cgctgaatat gggtcgccgt caggtatgga tgggcggtga agagttgatt
    18481 ctgacgccca aagaatatgc tctgctgtca cggttaatgc tcaaagcagg cagtccggta
    18541 catcgggaaa ttctctacaa cgacatctat aactgggaca atgaaccctc gaccaacacc
    18601 ctggaagtgc atatccacaa tctgcgcgac aaagtgggca aagcccgtat ccgcaccgtg
    18661 cgcggctttg gctatatgct ggtcgcgaat gaggaaaact aattgaatct gatgcgtttt
    18721 ctgcgccgac ctatatcgct gcgccaacgg ctgatattga ccatcggggc cattttgttg
    18781 gtgtttgagc tgatcagcgt cttctggcta tggcatgaaa gtaccgagca gattcagctg
    18841 tttgagcagg cgctacgcga caatcgcaac aacgatcgcc acatcatgcg tgagatccgc
    18901 gaggcggtcg ccagcctgat tgtccccggc gtctttatgg tcagcctgac gctatttatc
    18961 tgctatcagg cggtacgccg catcacccgc ccgctggcgg agctgcaaaa agagctggaa
    19021 gcgcgcaccg ccgacaactt aacgcccatt gccattcaca gcgccaccct cgaaatcgaa
    19081 gcggtggttt cggcgttgaa cgatctggtc agtcgcctga ccagcacgct ggataacgaa
    19141 aggttgttta ccgctgacgt cgcgcacgaa ctgcgaacgc cactggcggg ggtgcgtttg
    19201 catctggaac tgctggcgaa aacgcatcac attgatgtag caccgttagt ggcacggctt
    19261 gatcagatga tggagagcgt ctcccagctg ctgcaactgg cgcgtgccgg acagtcattt
    19321 tcttccggta attatcaaca tgtaaaactg ctggaagatg tcattctccc ctcgtatgac
    19381 gaactcagta ccatgctcga ccagcgacag caaaccctgc tattgccaga gagcgccgcc
    19441 gacatcaccg ttcagggtga tgcaaccctg ctgcggatgt tattgcgaaa cctggtagaa
    19501 aacgcccatc gttacagccc gcaaggcagc aacattatga ttaagctgca agaagatggc
    19561 ggagcggtca tggcagttga agatgaagga ccaggtattg atgagagtaa atgcggggag
    19621 ttgagtaaag cgtttgtacg tatggacagc cgttatggcg ggattggtct ggggttaagt
    19681 attgtcagcc gcattacaca gttgcatcac gggcagtttt tcctgcaaaa ccggcaagag
    19741 acttccggca cgcgggcctg ggtacggctg aagaaagatc agaacgtggc aaaccagata
    19801 taaagaaagc tgctgaccac cagcacgctg aacacggtag ttaaactttc ataaacacgg
    19861 tttttcattg cactctcctc cgttaacctg gaggagagta tgcgcgtcag agattaagtg
    19921 aaccttaaga gttcaattgg cctgataaga cagcgtcaca tcaggccatc cgtttcagct
    19981 tattcatcaa ttcgcggatg ttgctgcacc aggcgggtac gtttcgcctg caaatcggca
    20041 atctcgtggt caatattatc gattttctgc tcgatattat cgtaatgctc gacgagaatt
    20101 tccttcgctt cctgtatatc tgacgccgcc ggtgtcgcac ctttcaacgg acgatttgcc
    20161 gtctctttca tggttacgcc ggtgattaaa ccaatcaccg ccactaccat caggtaatag
    20221 gcaggcatca tcagattctg cgagctttcg accagccagg ccgccagtgt tggcgtcaga
    20281 ccggcaacca gcaccgaaat attaaatgcc gccgccagcg cgctgtaacg gatatgcgtc
    20341 gggaacatcg ctggcaaggt agaagccata acgcccataa agcagttaag gatcaccgcc
    20401 agcatcagta acccggcaaa aatcaggccg atgacgttac tgttaatcag aataaacgcc
    20461 gggatcgcca acacaaacag tgcaacacta ccaagtagca caaacggacg acggccaaaa
    20521 cggtcactca gcaagcccat caccggctgg acaaacagca taccgatcat aatggcgata
    20581 ataatcagca ccccgtggtc ttcggagtaa tgcaggttat gcgacaaata actcggcata
    20641 taggtcagca acatgtagta agtcacgttg gtggcaatta ccagaccaat acatgtcaac
    20701 aggctgcgcc agtatttagt ggcaatctct ttaaacgaga ctttcgggcc atcctgcaaa
    20761 ccttcgcggt cgccctgttc cagtttatca acatgctgct ggaacgccgg agtttcttcc
    20821 agcgcatggc gcaggtaaag cccgataatc cctaacggca gagcaataaa gaacggaata
    20881 cgccagcccc agtcgaggaa gttcgcttcg ccgacaatgg tcgaaattaa caccaccacg
    20941 cccgcaccca gcacaaaccc ggcaatagaa ccgaagtcca gccagctgcc cataaagcca
    21001 cgtttacggt caggggagta ttccgcaaca aatatcgacg ccccggtata ttcaccaccg
    21061 accgagaaac cttgtgccat cttacagatc aacagcagaa tcggtgccca aataccaatc
    21121 gtgtcgtagg acggtattaa gccaatacag aacgtactga tcgacataat cacaatagtg
    21181 atagcgagga tcttctggcg accatattta tcgcccaaca taccaaagaa gagtccgcca
    21241 agcggtcgaa tcagaaaggg aacggagaaa gtggcaagtg cagcaaccat ctgcacgctg
    21301 gggtcagccc ccgggaaaaa aactttacct aatgcgtaag caacaaaacc ataaacacca
    21361 aaatcaaacc attccattgc attacccagt gatgctgcgg taatggcttt acgcagttta
    21421 ccgtcatcaa taatggtgac atcacgaagg gtaatcggtt ttactttttt ccttttcagc
    21481 atagctttcc tcgcagagtt ggcccatttc cgcaaacgcc agaaaaatca gcgcgacggc
    21541 cgcaaaaggg gagcgggaat tgcaggatgc aatctctgta acaagcgtag cgggtttgct
    21601 tacaccctcc ggttaaggaa tgtacaatca aaccagttga cgcctgaatt gggcagttaa
    21661 tgaccttttt accctaccag tgtttaaatt tgtgatcaac ttcacacatg gatttcgttt
    21721 aggactcatt gatgtaactg attatttacg ccaaatttac agagaagatt tcctcgacca
    21781 ttgcccccac caaacgctgc gctgaacaga gtcgtggcat ccccagcgcg acagtttgcc
    21841 agcgttgggt cactgaccag caaacgggtt gtttatctgc cgtggtccac tttcccagcc
    21901 aacttaaaaa atcagcatga tccccgcccg tcggaacggt cgaagaggca aaccactgat
    21961 ggtaatagtg acgcgtagtc ggtgccgcat tgatactctc tgccagatgt tgtgtcgctt
    22021 tctcgcgcaa atcacccagc aacaacacca ccgcttgcgg gcttcccaga cggcaggcat
    22081 cgccaaatgc accccagcgc aactcctcca gaacgataaa acagcgccca ggcaacgacc
    22141 aggcgttata cgccccagca cgccagcggg taaaaatttg ctcgctctga tcacctgcct
    22201 gcaccagtag gcctcgttgc gttaacgcct tttccttcca gcgacaacgg gctgaaaacc
    22261 actcatacaa ggcggcaatt tgctggggga gctccgggaa agcagagtgc tggcggtgat
    22321 ccattttttg cagcagcgcc agatgctgcg cgatctgcgt cagcgccaga tgaccgggat
    22381 ccagcatcga cgccagtttt tccaccattg tcagtcgcag gctaagcggc gcatttaaca
    22441 tgccgccgtt cgtccatgtc tgaagctgca cacgcgaaag aagttcctgc atcagtcgtt
    22501 cacgaaactg ctgctgatgg gcttgaggat ggcgggcatc atcgccctgc gccagatcca
    22561 ctaaaaagcg cggatgaacc gactccagca cgcgaccggg gccgtccagt aacgtcttgg
    22621 tcaatatcgt tctgccgtga aaagtgtttg aatatcatcg cgtaacagct gagcgtcggt
    22681 gtaaatccag ctgtgagtca tcacagtctg ctgcaattgc tgctgcatca gcctgaccac
    22741 cgattcattt tgttgacgca gagccagact ttcgcgtaaa cgcgtctgta attctgtcaa
    22801 acatgaagcg aactcagcga aaaaagtatt catgcctgcc gtaacagatt catcgacctg
    22861 ctctgccaga actttagcca tttgttggca atagagatcg acttctgcgc ttaatgctcg
    22921 ttgcaacaca ctgtaatcaa ccgtttctgt cggggatttc tcatttcccc gtccccagtc
    22981 gggctgattc aaccagcgcg aaaaagtctc acgcacaacg cctaaacgcg tgctctgctc
    23041 gtccgttgct tcctggcgcg aaatgacttc actgaacagc tggcgagtgt tgaagtgggg
    23101 aactacgccg tgaaaaacag gaaaatgaaa cccaggacga aaccctgact cgctcaattc
    23161 cattttgact tgttgctcaa tggggcgaat aacatcggtt aacacccggc aaagggtgga
    23221 ttccatctcg gcaaaacgca gcgtaaagtc gcgactgatg gtgttctgcg ccgtctgtaa
    23281 cagtgtctca cagcgggtac gaatctcgct taacggctcc gaatcatcct gaaacaaggc
    23341 ggctaactgc gcattcaccg catcttgctg ttgacgcaga aagtggttgg cggaggtcag
    23401 ggccagctcg atttcatgtt taatctcgcc gctcacctgc gcctgattga gttgcaatag
    23461 ctgcaaactt tcttcgacct gatggatatt ttgccgcaat tgttcacaag cgacgtttaa
    23521 cccgtgcgca cgaaaatcca ggtattcccg cgcctgctgc gcgtaattca acagtttatg
    23581 cgcagcagat cgcagagcat acaatgaggc gttagcgtaa gcggcatgaa gcaacgcctg
    23641 aattggctgg gcgaacagcg aatcttccca caactgatcg gcagcatgac gaatatgttc
    23701 gaggtccgcc agatcggcat gacgccagcg cctgccgagc gcggcatggg caaaatcttc
    23761 cacccagcgt tgttgctctg gcgctggtaa cttaccgttg ttggctaact catagcgcgc
    23821 ccgattcgcc aggtagcccc acatcgacga caccgggaat atctgctgtg gcgtaataca
    23881 gcctttcatc agcgtcccgg aaatcagtgc ccgcacctgg tcggcgtcgt cactgttacg
    23941 atcctgttga tcgaacttat tgaccagcac atacagcggc accgattgcc ccaccgccaa
    24001 aatcgcctca cggacctctt catcggagat cgatttcagt tgcgtataat ccagcaccgc
    24061 cagtaccgcc gaggcgcgtg ccagctgctg gttaagcatt ttttgcagat gcggttgccc
    24121 ggcttcattt ggcccggggg tatccagtaa cgtcaactga ccgggataac tttccagccc
    24181 cgccagatgg acaaactcca cttcaatcac gggaatatgc tcaatggcgg cgtaagcaga
    24241 aaaaggaaaa tcgacgtcca gcgccttcgc cagtcgcact aaatcattca aacttttcag
    24301 acaatgaaaa ataggctggg cacccagata atatttttcg aaagcgacgc cattttcgat
    24361 ccgctgcata agcgcacgca tatctttatc tatttccagc acatcggtca gatgcttaat
    24421 atcgcaatca cgcaggcgct gttgtaattg ttgaattaaa caatcgattg gcgcgacatg
    24481 tgaaaaatgc agtaccggtt ccttttgccc gggcgtatgg cgaataagcg tcggcagcgc
    24541 agtcattggg cgattacgat taggcagaac ctccgtacca acaatggcat taatggtggt
    24601 tgatttccct gctttcatgg taccgacaat tgccagcacc atttccagtc gggaaatttt
    24661 acacaactca ttattcagca tcgcgtgacg ttcggcgata ttaggctgac tccagggtaa
    24721 agccagttgt ggcgcgtcgt ctccgggtac agagagaggc attttttcca gtaactgcaa
    24781 ctgttggcga gaaagctgta acaggcgttc agcctcctga cttaactcat acagggtctg
    24841 tgtgtacata gaaaattctt ccttaaagca aattttgtta cttttattta gccagattgt
    24901 ttttgagttc tgttttcggc ttttataatt actgcaagaa ataattttat atttagtgtg
    24961 ttgttttttt atcagaataa ataacgtctt ctgatgcgtt taaaacgtca gaaagataaa
    25021 aatatcatgt gaataaaaaa aagaacaagt agagcattaa cattatctta aataataaat
    25081 agaggcaaaa agattatttt ctttttgcgt ttcctttcaa atgaaaacga tcgtcgtcta
    25141 aaatcagcag tacccccgac aaactcaggg attttgtgta taattgcggc ctttttcggc
    25201 aatctgccgt tttttggcgc ttttgccctg ctgacttttg aggaaatcca catgtcatta
    25261 ccacactgcc caaaatgcaa ctccgaatac acttacgaag ataacggcat gtacatctgc
    25321 ccggaatgtg cctacgaatg gaacgacgca gaacctgcac aggaaagcga cgagctgatc
    25381 gttaaagatg ctaacggcaa tctgctggct gacggcgaca gcgttaccat cattaaagat
    25441 ctgaaggtga aaggtagctc ttcgatgctg aaaattggca ccaaagtgaa aaacatccgc
    25501 ctggttgaag gcgaccataa catcgattgc aaaatcgacg gttttggtcc gatgaaactg
    25561 aaatctgagt ttgtgaaaaa gaactgattg tattgtgatc ggtaagccgg ataaggcgct
    25621 cgcgccgcat ccggcaacga tgccgactgc ctgatgcgac gcttgcgcgt cttatcaggc
    25681 agcctctcat ctgtataaat ttcgaactac acttaactgg cttctcttaa ctgaggtcac
    25741 catcatgccg ttaagtccct acctctcttt tgccggtaac tgttccgacg cgattgccta
    25801 ttatcaacgt acgttgggcg cggaactgct ctataaaatc agcttcggcg aaatgccgaa
    25861 atcagcgcag gacagcgccg agaactgccc ttccggaatg caatttcccg ataccgccat
    25921 cgctcatgcc aacgtgcgca ttgccggaag cgacatcatg atgagcgatg ctataccgtc
    25981 aggaaaagcc agctactccg gctttacgct ggtgctcgat tcgcaacagg tcgaagaagg
    26041 aaaacgctgg tttgataacc ttgccgctaa cggaaaaatc gaaatggcct ggcaggaaac
    26101 tttctgggcg catggctttg gcaaagtcac cgataaattt ggcgtaccgt ggatgattaa
    26161 tgtcgtcaaa caacaaccaa cgcaataacc cgccgggagg cccgccctcc cgcactgtca
    26221 tcgaattccc gttaactctt catctgttag tcacttttaa ttaaccaaat cgtcacaata
    26281 atccgccacg atggagccac ttttttaggg aggctgcatc atgcaaacga ttatccgtgt
    26341 cgagaagctc gccaaaacct tcaatcagca tcaagcgctg catgcggttg atctgaacat
    26401 tcatcacagt gaaatggtgg ctctgcttgg gccgtcgggt tccggcaaat ccaccctttt
    26461 acgtcattta agcggtttga ttaccggcga taaatccgcc ggtagccata tcgagctact
    26521 gggccgcact gtccagcgcg aaggccgcct ggcccgcgat atccgcaaaa gccgcgccaa
    26581 caccggctac atctttcaac aattcaacct ggtgaaccgc ctgagcgtac tggagaacgt
    26641 gctgattggc gcgctcggca gcacgccgtt ctggcgcacc tgttttagct ggttcaccgg
    26701 cgagcagaaa cagcgcgcgt tacaggcgct gacccgcgtt ggcatggtgc attttgccca
    26761 tcagcgcgtt tccaccctct ccggcggcca gcagcaacgt gtggcgattg cccgtgcgct
    26821 gatgcagcag gcgaaagtga ttctggccga tgaacccatc gcctcgctgg acccggaatc
    26881 agcgcgcatc gtgatggaca ccctgcgcga catcaaccag aacgacggca tcaccgtggt
    26941 cgtcacgctg catcaggtgg attacgccct gcgctactgc gaacgcatcg tcgccctgcg
    27001 tcaggggcac gtcttctacg acggcagcag ccaactgttt gataacgaac gttttgacca
    27061 tctctaccgc agcattaacc gcatcgaaga gaacgcgaaa gctgcctgat atccccatca
    27121 ttgaggaaaa cgaatgaacg ctaagataat tgcctcgctg gccttcacca gcatgttcag
    27181 cctcagcacc ctgttaaccc cggcacacgc cgaagagcag gaaaaggcgc tgaatttcgg
    27241 cattatttca acggaatcac agcaaaacct gaaaccgcaa tggacgccgt ttttacagga
    27301 tatggagaag aaactgggcg tgaaggtcaa cgccttcttt gccccggact acgcgggcat
    27361 tatccagggg atgcgcttca ataaagtgga tattgcctgg tacggcaacc tgtcggcgat
    27421 ggaagcggtg gatcgcgcca atggccaggt cttcgcccag acggtcgcgg cggatggatc
    27481 gccgggttac tggagcgtgt tgatcgtcaa caaagacagc ccgatcaaca acctgaacga
    27541 tctgctggcg aagcggaaag atctcacctt cggcaatggc gatcctaact ccacctctgg
    27601 cttcctcgtc cccggttact acgtcttcgc taaaaacaat atctccgcca gcgacttcaa
    27661 gcgcaccgtc aacgccgggc atgaaaccaa cgcgctggcc gtcgccaaca agcaagtgga
    27721 tgttgccacc aacaacaccg aaaacctcga caagctgaaa acctccgcgc cggagaagct
    27781 taaagagctg aaggtgatct ggaaatcgcc gctgatccta ggcgatccga tcgtctggcg
    27841 caagaatctg tccgaaacta ccaaagacaa gatctacgac ttctttatga actacggaaa
    27901 aacgccggaa gagaaagcgg tgctggaacg cctgggctgg gcgccgttcc gcgcttccag
    27961 cgacctgcaa ctggtgccga ttcgccagct cgcactgttt aaagagatac aaagcgtgaa
    28021 aggcaataaa gggctgaatg agcaggacaa gctggcgaaa accaccgaga ttcaggcgca
    28081 gctggatgac ctggaccgcc tgaacaacgc gttaagcgcg atgagttcgg tgagtaaagc
    28141 ggtgcagtaa atcgtaggtc ggataagacg ccccggcgtc gcatccgaca atgtgtaggc
    28201 gttgatgccg gatgcggtgc aagcacctta tccggcctac agaccggagc caaacatgca
    28261 aaccatcacc atcgccccac ccaagcgtag ctggttctcg cttctgagct gggccgttgt
    28321 tctcgacgtg ctggtcgtct cgtggcaggg cgcggaaatg gccccgctta cgctgattaa
    28381 agacggcggc aacatggcaa cctt
//
LOCUS       DAJTRC010000053        28149 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.053, whole genome shotgun sequence.
ACCESSION   DAJTRC010000053 DAJTRC010000000
VERSION     DAJTRC010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 28149)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28149)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28149
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.053"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="OO357_002367"
                     /pseudo
     CDS             complement(41..1036)
                     /gene="yjfF"
                     /locus_tag="OO357_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418652.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease YjfF"
     gene            complement(1023..2045)
                     /locus_tag="OO357_002368"
     CDS             complement(1023..2045)
                     /locus_tag="OO357_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5887286.1"
                     /translation="MPQSLPDTTPPKRRFHWPTGMPQLAALLLVLLVDSLVAPHFWQV
                     VLQDGRLFGSPIDILNRAAPVALLAIGITLVIATGGIDLSVGAVMAIAGATTAAMTVA
                     GFSLPIVLLSALGTGILAGLWNGILVAILKIQPFVATLILMVAGRGVAQLITAGQIVT
                     FNSPDLSWFGSGSLLFLPTPVIIAVLTLLLFWLLTRKTALGMFIEVVGINIRAAKNAG
                     VNTRIIVMLTYVLSGLCAAIAGIIVAADIRGADANNAGLWLELDAILAVVIGGGSLMG
                     GRFNLLLSVVGALIIQGMNTGILLSGFPPEMNQVVKAVVVLCVLIVQSQRFISLIKGV
                     RSHDKT"
     gene            complement(2059..3561)
                     /locus_tag="OO357_002369"
     CDS             complement(2059..3561)
                     /locus_tag="OO357_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HCR5887287.1"
                     /translation="MTTDQHQEILRTEGLSKFFPGVKALDNVDFSLRRGEIMALLGEN
                     GAGKSTLIKALTGVYHADRGTIWLEGQTISPKNTAHAQQLGIGTVYQEVNLLPNMSVA
                     DNLFIGREPKRFGLLRRKEMEKRATELMASYGFSLDVREPLNRFSVAMQQIVAICRAI
                     DLSAKVLILDEPTASLDTQEMELLFGLMRQLRDRGVSLIFVTHFLDQVYQVSDRITVL
                     RNGSFVGCRETRELPQIELVKMMLGRELDTHALQRAGRTLLSDKPVAAFKNYGKKGTI
                     APFDLEVRPGEIVGLAGLLGSGRTETAEVIFGIKPADSGTALIKGKPQTLRSPHQASV
                     LGIGFCPEDRKTDGIIAAASVRENIILALQAQRGWLRPISRKEQQEIAERFIRQLGIR
                     TPSTEQPIEFLSGGNQQKVLLSRWLLTRPQFLILDELTRGIDVGAHAEIIRLIETLCA
                     DGLALLVISSELEELVGYADRVIIMRDRKQVAEIPLAQLSVPAIMNAIAA"
     gene            complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="OO357_002370"
                     /pseudo
     CDS             complement(3701..4657)
                     /gene="ytfQ"
                     /locus_tag="OO357_002370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313232.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter
                     substrate-binding protein YtfQ"
     gene            4967..5497
                     /gene="ppa"
                     /locus_tag="OO357_002371"
     CDS             4967..5497
                     /gene="ppa"
                     /locus_tag="OO357_002371"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001739810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HCR5887288.1"
                     /translation="MSLLNVPAGKDLPEDIYVVIEIPANADPIKYEIDKESGALFVDR
                     FMSTAMFYPCNYGYINHTLSLDGDPVDVLVPTPYPLQPGSVIRCRPVAVLKMTDEAGE
                     DAKLVAVPHSKLSKEYDHIKDVNDLPELLKAQIAHFFEHYKDLEKGKWVKVEGWENAE
                     AAKAEIVASFERAKNK"
     gene            complement(5877..6218)
                     /locus_tag="OO357_002372"
     CDS             complement(5877..6218)
                     /locus_tag="OO357_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HCR5887289.1"
                     /translation="MRIFVYGSLRHKQGNSHWMTNAQLLGDFSIDNYQLYSLGHYPGA
                     VPGNGTVHGEVYRIDNATLAELDALRTRGGEYARQLIQTPYGSAWMYVYQRPVDGLKL
                     IESGDWLDRDK"
     gene            complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="OO357_002373"
     CDS             complement(6221..10000)
                     /gene="tamB"
                     /locus_tag="OO357_002373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamB"
                     /protein_id="HCR5887290.1"
                     /translation="MSLWKKISLGVVIVILLLLGSVAFLVGTTSGLHLVFKAADRWVP
                     GLDIGKVTGGWRDLTLSDVRYEQLGVAVKAGNLHLGVGLECLWNSSVCINDLALKDIQ
                     VNIDSKKMPPSEQVEEEEDSGPLDLSTPYPITLTRVALDNVNIKIDDTTVSVMDFTSG
                     LNWQEKTLTLKPTSLKGLLIALPKVVEVAQEEVVEPKIENPQPEEKPLGETLKDLFSR
                     PVLPEMTDVHLPLNLNIEEFKGEQLRVTGDTDITVRTMLLKVSSIDGNTKLDALDIDS
                     NQGIVNASGTAQLSDNWPVDITLNSTLNVEPLKGEKVKLKVGGALREQLEIGVNLSGP
                     VDMDLRAQTRLAEAGLPLNVEVNSKQLYWPFTGEKQYQADDLKLKLTGKMTDYTLSMR
                     TAVKGQEIPPATITLDAKGNEQQVNLDKLTVAALEGKTELKALLDWQQAISWRGELTL
                     NGINTAKEFPEWPSKLNGLIKTRGSLYGGTWQMEVPELKLTGNVKQNKVNVDGTLKGN
                     SYMQWMIPGLHLELGPNSAEVKGELGVKDLNLDATINAPGLDNALPGLGGTAKGLVKV
                     RGTVEAPQLLADITARGLRWQELSVAQVRVEGDIKSTDQIAGKLDVRVEQISQPDVNI
                     NLVTLNAKGSEKQHELQLRIQGEPVSGQLNLAGSFDRKEERWKGTLSNTRFQTPVGPW
                     SLTRDIALDYRNKEQKISIGPHCWLNPNAELCVPQTIDAGAEGRAVVNLNRFDLAMLK
                     PFMPETTQASGIFTGKADVAWDTTKEGLPQGSITLSGRNVQVTQTVNDAALPVAFQTL
                     NLTAELRNNRAELGWTIRLTNNGQFDGQVQVTDPQGRRNLGGNVNIRNFNLAMINPIF
                     TRGEKAAGMVSANLRLGGDVQSPQLFGQLQVTGVDIDGNFMPFDMQPSQLAVNFNGMR
                     STLAGTVRTQQGEIYLNGDADWSQIENWRARVTAKGSKVRITVPPMVRMDVSPDVVFE
                     ATPNLFTLDGRVDVPWARIVVHDLPESAVGVSSDVVMLNDNLQPEEPKTASIPINSNL
                     IVHVGNNVRIDAFGLKARLTGDLNVVQDKQGLGLNGQINIPEGRFHAYGQDLIVRKGE
                     LLFSGPPDQPYLNIEAIRNPDATEDDVIAGVRVTGLADEPKAEIFSDPAMSQQAALSY
                     LLRGQGLESDQSDSAAMTSMLIGLGVAQSGQIVGKIGETFGVSNLALDTLGVGDSSQV
                     VVSGYVLPGLQVKYGVGIFDSIATLTLRYRLMPKLYLEAVSGVDQALDLLYQFEF"
     gene            complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="OO357_002374"
     CDS             complement(9997..11730)
                     /gene="tamA"
                     /locus_tag="OO357_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex protein TamA"
                     /protein_id="HCR5887291.1"
                     /translation="MRYIRQLCCVSLLCLSGSAVAANVRLQVEGLSGQLEKNVRAQLS
                     TIESDEVTPDRRFRARVDDAIREGLKALGYYQPTIEFDLRPPPKKGRQVLIAKVTPGV
                     PVLIGGTDVVLRGGARTDKDYLKLLDTRPAIGTVLNQGDYENFKKSLTSIALRKGYFD
                     SEFTKAQLGIALGLHKAFWDIDYNSGERYRFGHVTFEGSQIRDEYLQNLVPFKEGDEY
                     ESKDLAELNRRLSATGWFNSVVVAPQFDKARETKVLPLTGVVSPRTENTIETGVGYST
                     DVGPRVKATWKKPWMNSYGHSLTTSTSISAPEQTLDFSYKMPLLKNPLEQYYLVQGGF
                     KRTDLNDTESDSTTLVASRYWDLSSGWQRAINLRWSLDHFTQGEITNTTMLFYPGVMI
                     SRTRSRGGLMPTWGDSQRYSIDYSNTAWGSDVDFSVFQAQHVWIRTLYDRHRFVTRGT
                     LGWIETGDFDKVPPDLRFFVGGDRSIRGYKYKSIAPKYANGDLKGASKLITGSLEYQY
                     NVTGKWWGAVFVDSGEAVSDIRRSDFKTGTGVGVRWESPVGPIKLDFAVPVADKDEHG
                     LQFYIGLGPEL"
     gene            11936..12574
                     /gene="msrA"
                     /locus_tag="OO357_002375"
     CDS             11936..12574
                     /gene="msrA"
                     /locus_tag="OO357_002375"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HCR5887292.1"
                     /translation="MSLFDKKHLVSPADALPGRNTPMPVATLHAVNGHSMTNVPDGME
                     IAIFAMGCFWGVERLFWQLPGVYSTAAGYTGGYTPNPTYREVCSGDTGHAEAVRIVYD
                     PSVISYEQLLQVFWENHDPAQGMRQGNDHGTQYRSAIYPLTPEQDAAARASLERFQAA
                     MLAADDDRHITTEIANATPFYYAEDDHQQYLHKNPYGYCGIGGIGVCLPPEA"
     gene            12897..14240
                     /locus_tag="OO357_002376"
     CDS             12897..14240
                     /locus_tag="OO357_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5887293.1"
                     /translation="MLNSILVILCLIAVSAFFSMSEISLAASRKIKLKLLADEGNINA
                     QRVLNMQENPGMFFTVVQIGLNAVAILGGIVGDAAFSPAFHSLFSRYMSAELSEQLSF
                     ILSFSLVTGMFILFADLTPKRIGMIAPEAVALRIINPMRFCLYVCTPLVWFFNGLANM
                     IFRIFKLPMVRKDDITSDDIYAVVEAGALAGVLRKQEHELIENVFELESRTVPSSMTP
                     RENVIWFDLHEDEQSLKNKVAEHPHSKFLVCNEDIDHIIGYVDSKDLLNRVLANQSLA
                     LNSGVQIRNTLIVPDTLTLSEALESFKTAGEDFAVIMNEYALVVGIITLNDVMTTLMG
                     DLVGQGLEEQIVARDENSWLIDGGTPIDDVMRVLDIDEFPQSGNYETIGGFMMFMLRK
                     IPKRTDSVKFAGYKFEVVDIDNYRIDQLLVTRIDSKATALSPKLPDAKDKEESVA"
     gene            complement(14302..14508)
                     /locus_tag="OO357_002377"
     CDS             complement(14302..14508)
                     /locus_tag="OO357_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1107 domain-containing protein"
                     /protein_id="HCR5887294.1"
                     /translation="MKIFQRYNPLQVAKYVKILFRGRLYIKDIGAFEFDKGKILIPKV
                     KDKLHLSVMSEVNRQVMRLQTEMA"
     gene            14833..15387
                     /locus_tag="OO357_002378"
     CDS             14833..15387
                     /locus_tag="OO357_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtfJ family protein"
                     /protein_id="HCR5887295.1"
                     /translation="MTLRKILALTCLLLPMMASAHQFEIGQRVPPIGITDRGELVLDK
                     DQFSYKTWNSAQLVGKVRVLQHIAGRTSAKEKNATLIEAIKSAKLPHDRYQTTTIVNT
                     DDAIPGSGMFVRSSLESNKKLYPWSQFIVDSNGVARGAWQLDEESSAVVVLDKDGRVQ
                     WAKDGALTPEEVQQVMDLLQKLLK"
     gene            complement(15450..16388)
                     /locus_tag="OO357_002379"
     CDS             complement(15450..16388)
                     /locus_tag="OO357_002379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887296.1"
                     /translation="MLPRIRHNNFIGAVELFVKSSHTKTHSNDFFNNIQHAFKKKDWV
                     SNYDSLLTLRESFRCATQIDKNSYQVLSSKNETVNAMDNFLISFSLKDNGAEYTITLR
                     GSGFEYEEIPITINEYNSFMDFKNREFPLEQNRRLYACDILQKKQSDIPKRIKGYIRQ
                     AFGDVSFGYALLEDVVSKLKRGKFELQIPGGGIKECDGWYIYEKIIDDNFAIVIESLG
                     FALKIYGGDERFRNGSSVVLEDEDYSLIYNFLVNAGCQQVELAEQVDAIVSANLAADS
                     NITKEKICEKYKSTIEAFKKEQLALPVLVRCKNSET"
     gene            complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="OO357_002380"
     CDS             complement(16600..17340)
                     /gene="cysQ"
                     /locus_tag="OO357_002380"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HCR5887297.1"
                     /translation="MLDQVCQLARNAGDAIMQVYDGTKPMDVVSKADNSPVTAADIAA
                     HTVIMDGLRTLAPDIPVLSEEDPPGWEVRQHWQRYWLVDPLDGTKEFIKRNGEFTVNI
                     ALIDHGKPILGVVYAPVMNVMYSAAEGKAWKEECGVRKLIQVRDARPPLVVISRSHAD
                     AELKEYLQQLGEHQTTSIGSSLKFCLVAEGQAQLYPRFGPTNIWDTAAGHAVAAAAGA
                     HVHDWQGKPLDYTPRESFLNPGFRVSIY"
     gene            17530..19473
                     /gene="cpdB"
                     /locus_tag="OO357_002381"
     CDS             17530..19473
                     /gene="cpdB"
                     /locus_tag="OO357_002381"
                     /EC_number="3.1.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2',3'-cyclic-nucleotide 2'-phosphodiesterase"
                     /protein_id="HCR5887298.1"
                     /translation="MIKFSATLLATLIAASVNAATVDLRIMETTDLHSNMMDFDYYKD
                     TATEKFGLVRTASLINDARNEVKNSVLVDNGDLIQGSPLADYMSAKGLKAGDVHPVYK
                     ALNTLDYTVGTLGNHEFNYGLDYLKNALAGAKFPYVNANVIDARTKQPMFTPYLIKDT
                     EVVDKDGKKQTLKIGYIGVVPPQIMGWDKANLSGKVTVNDITETVRKYVPEMREKGAD
                     VIVVLAHSGLSADPYKVMAENSVYYLSEIPGVNAIMFGHAHAVFPGKDFADIEGADIT
                     KGTLNGVPAVMPGMWGDHLGVVDLQLSNDSGKWQVTQAKAEARPIYDIANKKSLAAED
                     SKLVETLKADHDATRQFVSKPIGKSADNMYSYLALVQDDPTVQVVNNAQKAYVEHYIQ
                     GDPDLAKLPVLSAAAPFKVGGRKNDPASYVEVEKGQLTFRNAADLYLYPNTLIVVKAS
                     GKEVKEWLECSAGQFNQIAPNSTKPQSLINWDGFRTYNFDVIDGVNYQIDVTQPARYD
                     GECQMINANAERIKNLTFNGKPIDPNAMFLVATNNYRAYGGKFAGTGDSHIAFASPDE
                     NRSVLAAWIADESKRAGEIHPAADNNWRLAPIAADKKLDIRFETSPSDKAAAFIKEKG
                     QYPMNKVATDDIGFAIYQVDLSK"
     gene            complement(19599..19979)
                     /locus_tag="OO357_002382"
     CDS             complement(19599..19979)
                     /locus_tag="OO357_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HCR5887299.1"
                     /translation="MSQVSLSQQLKEGNLFAEQCPSREVLKHVTSRWGVLILVALREG
                     THRFSDLRRKIGGVSEKMLAQSLQALEQDGFLNRIAYPVVPPHVEYSLTPLGEQVSEK
                     VAALADWIELNLPEVLAVRDERAA"
     gene            20068..20928
                     /gene="qorB"
                     /locus_tag="OO357_002383"
     CDS             20068..20928
                     /gene="qorB"
                     /locus_tag="OO357_002383"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5887300.1"
                     /translation="MIAITGATGQLGHYIIESLMKTVPASQIVAIVRNPAKAQALAAQ
                     GITVRQADYSDEAALTSALQGVEKLLLISSSEVGQRAPQHRNVINAAKAAGVKFIAYT
                     SLLHADTSPLGLADEHIETEKMLADSGIVYTLLRNGWYSENYLASAPAALEHGVFIGA
                     AGDGKIASATRADYATAAARVISEAGHEGKVYELAGDSAWTLTQLAAELTKQSGKQVT
                     YQNLSEADFAAALKSVGLPDGLADMLADSDVGASKGGLFDNSKTLSKLIGCPTTTLAE
                     SVSHLFNVNN"
     gene            21037..22002
                     /locus_tag="OO357_002384"
     CDS             21037..22002
                     /locus_tag="OO357_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709987.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5887301.1"
                     /translation="MISGVLYALLAGLMWGLIFVGPLIVPEYPAMLQSMGRYLALGLI
                     ALPIAWLGRVRLRQLARRDWLTALMLTMMGNLIYYFCLASAIQRTGAPVSTMIIGTLP
                     VVIPVFANLLYSQRDGKLAWGKLAPALICIGIGLASVNIAELNHGLPDFDWARYTSGI
                     VLALVSVVCWAWYALRNARWLRENPDKHPMMWATAQALVTLPVSLIGYLVACYWLNIQ
                     TPDFSLPFGPRPLVFISLMVAIAVLCSWVGALCWNVASQRLPTVILGPLIVFETLAGL
                     LYTFLLRQQMPPLMTLSGIALLVIGVVIAVRAKPEKPLTESVSES"
     gene            22110..22772
                     /gene="ytfE"
                     /locus_tag="OO357_002385"
     CDS             22110..22772
                     /gene="ytfE"
                     /locus_tag="OO357_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster repair protein YtfE"
                     /protein_id="HCR5887302.1"
                     /translation="MAYRDQPLGELALSIPRASALFRKYDMDYCCGGKQTLARAAARK
                     ELDVEVIEAELAKLAEQPIEKDWRSAPLAEIIDHIIVRYHDRHREQLPELILQATKVE
                     RVHADKPSVPKGLTKYLTMLHEELSSHMMKEEQILFPMIKQGMGSQAMGPISVMESEH
                     DEAGELLEVIKHTTNNVTPPPEACTTWKAMYNGINELIDDLMDHISLENNVLFPRALA
                     GE"
     gene            complement(22817..24229)
                     /gene="cycA"
                     /locus_tag="OO357_002386"
                     /gene_synonym="dagA"
     CDS             complement(22817..24229)
                     /gene="cycA"
                     /locus_tag="OO357_002386"
                     /gene_synonym="dagA"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-serine/D-alanine/glycine transporter"
                     /protein_id="HCR5887303.1"
                     /translation="MVDQVKVVADDQAPAEQSLRRNLTNRHVQLIAIGGAIGTGLFMG
                     SGKTISLAGPSIIFVYMIIGFMLFFVMRAMGELLLSNLEYKSFSDFASDLLGPWAGYF
                     TGWTYWFCWVVTGMADVVAITAYAQFWFPDLSDWVASLAVIVLLLTLNLATVKMFGEM
                     EFWFAMIKIVAIVSLIVVGLVMVAMHFQSPTGVEASFAHLWNDGGWFPKGLSGFFAGF
                     QIAVFAFVGIELVGTTAAETKDPEKSLPRAINSIPIRIIMFYVFALIVIMSVTPWSSV
                     VPEKSPFVELFVLVGLPAAASVINFVVLTSAASSANSGVFSTSRMLFGLAQEGVAPKA
                     FAKLSKRAVPAKGLTFSCICLLGGVVMLYVNPSVIGAFTMITTVSAILFMFVWTIILC
                     SYLVYRKQRPHLHEKSIYKMPLGKLMCWVCMAFFVFVVVLLTLEDDTRQALLVTPLWF
                     IALGLGWLFIGKKRAAELRK"
     gene            complement(24537..25157)
                     /gene="fklB"
                     /locus_tag="OO357_002387"
     CDS             complement(24537..25157)
                     /gene="fklB"
                     /locus_tag="OO357_002387"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015742582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HCR5887304.1"
                     /translation="MTTPTFDTIEAQASYGIGLQVGQQLSESGLEGLLPEALVAGIAD
                     ALEGKHPAVPVDVVHRALREIHERADAVRRQRFQAMAAEGVKYLEENAKKEGVNSTES
                     GLQFRVINQGEGAIPARTDRVRVHYTGKLIDGTVFDSSVARGEPAEFPVNGVIPGWIE
                     ALTLMPVGSKWELTIPQELAYGERGAGASIPPFSTLVFEVELLEIL"
     gene            25376..25921
                     /locus_tag="OO357_002388"
     CDS             25376..25921
                     /locus_tag="OO357_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OapA family protein"
                     /protein_id="HCR5887305.1"
                     /translation="MPGRFELKPTLEKVWHAPDNFRFMDPLPPMHRRGIIIAAIVLVV
                     GFLLPSDDTPNASVVTREAQLDIQSQSQPPTEEQPQTTQTQPFQPDSGIDNQWRSYRV
                     EPGKTMAQLFRDHGLPATDVYAMAQVEGAGKPLSNLQNGQMVKIRQNASGVVTGLTID
                     TGNNQQVLFTRQPDGSFIRAR"
     gene            complement(25905..26552)
                     /locus_tag="OO357_002389"
     CDS             complement(25905..26552)
                     /locus_tag="OO357_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709992.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5887306.1"
                     /translation="MYIDTSNVQSLKEKLLLCAVNEFAEYGYEGARVDNIVKAAGCSK
                     QTVYHHFGNKENLFIEVLEYTWNDIRQKEKALDFSDLPPQKAIEKIIDFTWDYYIANP
                     WFLKIVHSENQSKGVHYAKSQRLLEINHAHLQLMESLLDEGKKYNIFKPGIDPLQVNI
                     NIAALGGYYLINQHTLGLVYHISMVSPQALEARRKVIKETILSWLLVDPSSTAHE"
     gene            26764..27555
                     /locus_tag="OO357_002390"
     CDS             26764..27555
                     /locus_tag="OO357_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HCR5887307.1"
                     /translation="MKSTRPVAVITGATRGIGKGCAQELARGGFNLLINDRPDADSVE
                     KLHITQQECLAEGVEVICFPADVGDLSLHEEMLDAAQNQWGRLDCLLNNAGISVKKRG
                     DLLDLEPDSFDQNIAINTRAPFFLAQAFSKRLLAQPKPEAELPHRSIIFVSSINAIML
                     AMNRGEYTIAKTAVSAAARLFAARLCNEQIGVYEVRPGLIKTDMTIPATAYYDELIAK
                     GLVPWGRWGYPADIASTVRAMAEGKLIYTCGQAVAIDGGLSMPRF"
     gene            27565..>28149
                     /locus_tag="OO357_002391"
     CDS             27565..>28149
                     /locus_tag="OO357_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001205218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HCR5887308.1"
                     /translation="MRDLLQRPDLFSINTATLGYKTPLPAIIDACAARGIGAIAPWRR
                     ELQGEDLQQITRQLAASNMSVSGLCRSTYYTAPTLAERKLAIDDNRRALDDAAVLNAA
                     CYMQVVGGLPTGTKDLYEAREQVKQGIRQLLPHSKDVGVPIALEPLHPMTAADRSCLC
                     TLRQALDWCDELDPDGEFGLGVAVDVYHVWWDPDL"
BASE COUNT         6909 a         7501 c         6936 g         6803 t
ORIGIN      
        1 tccttaactg atcggcatta ctccacacgg agaggcattt ttatcgctgc gcaatgttga
       61 ctcttgtcac tggcgaactc tgacgattct cccacagcac cgttaaccca cgctgtaatg
      121 ctataaaaat aaacaacaaa ataccgatgg cgattttcgt ccactaggaa ctcagcgtgc
      181 cgtcaaagtt gatgtaagtc tggatcagtc cctgaattgc cacgccaaaa agcgtcccaa
      241 gcaccgtccc gacgccacca cttaacaacg taccgccaat caccaccgac gcgatggcgt
      301 ccagttccac acctacgccc gccagtgcat atccggcttg ggtataaatc gagaagacaa
      361 tccccgccag tgttgccagt ccggtggaga gcatataaat gcgaatagtg gtgctgcgag
      421 tagaaatccc catcaggttc gccgacgtcg cgttgccgcc aatagcgtac acctgattgc
      481 caaaacgggt acaatgcgca aggaaaatac cgataaccac caccgccagc atcaacagcc
      541 ccatcgcact aaggcgaccg ccgccgggga ttttccacgc aaggcttgag agcgtgtcat
      601 aaatgggatg gtttatcgga atcgactctt ccgaaacgag atagctgacg ccgcgcaaaa
      661 agaacatccc cgcaagcgta atgataaatg ccgggatctt cagggcgtcg atcagaagcc
      721 ccataaatgc gccgaaggca cagcccatca ccagcactag cggaaacgcc agcagcggcg
      781 agaggccgaa atcgccaacc acttttgcca gaaacacgcc agtaaaggcg atcaccgaac
      841 cgacggagag atcgataccg ccagagagga tcacaaaggt catgccaacg gcgattatcc
      901 caagaaaggc gttatcggtc aggatattgc agatcaccct tgtggaagca aaaccgggaa
      961 actgggtcag gcagtagaga tagcccagca caaagacgcc gatggtgatc atcagcggca
     1021 agttacgttt tatcatggct acgtactcct ttaatcagac tgataaagcg ttgcgactga
     1081 acaatcagca cacaaagcac caccaccgct ttcaccacct ggttcatctc cggcggaaag
     1141 cccgaaagca aaattccggt gttcatcccc tgaataatca gcgcccccac taccgagagc
     1201 agcaggttaa agcgcccgcc catcagcgat ccgccgccaa tcaccaccgc gagaatggcg
     1261 tccagctcca gccataatcc ggcgttgttg gcatcggcac cgcgaatatc cgccgccacg
     1321 ataatgcccg caatcgccgc acatagcccg ctcaacacgt aggtgagcat gacgatgatt
     1381 cgcgtgttta ccccggcatt ttttgccgcc cgaatgttga taccaacgac ttcgataaac
     1441 atccccagcg ccgttttgcg ggtcaacagc cagaacagga gaagcgtcag caccgcgata
     1501 atgaccggcg ttggcaggaa caacagcgat ccgctgccaa accatgagag atccggcgag
     1561 ttaaacgtga cgatctgccc ggcggtgatc agttgcgcca cgccgcgccc ggcgaccatc
     1621 aggatcaggg tggcgacaaa cggctgaatt ttgaggatcg ctaccagtat gccgttccac
     1681 aatcccgcca ggatgccggt gcccagggcg cttaacaaaa caatcggcag gctgaatccc
     1741 gcgaccgtca tcgcagccgt tgtggctccg gcgatagcca tcaccgcccc cacagagaga
     1801 tcgataccac cggtggcgat caccagcgta ataccaatcg ccagtaacgc aacgggagct
     1861 gcacggttaa gaatgtcgat agggctaccg aacaaacgcc catcctgaag caccacctgc
     1921 cagaaatgcg gggctaccag gctatcgacc agcagcacca gcaacagtgc cgccagctgc
     1981 ggcatccccg tcggccagtg aaaacgcctt tttggcggcg tagtgtcagg gagagattga
     2041 ggcatcacac tgttctcctt acgccgcaat ggcgttcatg atcgccggaa cggaaagctg
     2101 tgccagcggg atctccgcca cctgtttgcg atcgcgcatg atgatcaccc ggtcggcata
     2161 gcccaccagc tcttccagtt cggaggagat caccagcagc gccagaccgt cagcacacag
     2221 cgtttcaatc aggcggatga tctcggcgtg ggcaccaaca tcaatgccac gggttagctc
     2281 atcaaggatc agaaattgcg gtcgggtcag tagccaacgt gaaagcagca ctttttgctg
     2341 attgccaccg gagagaaatt caatcggttg ttcagttgaa ggtgtgcgaa tgccaagctg
     2401 gcggataaag cgttcggcaa tctcttgctg ctctttgcgg gaaatgggtc gtagccagcc
     2461 gcgctgggcc tggagagcga ggatgatatt ttcccgcacc gaggcggcgg cgatgatgcc
     2521 atcggttttc ctgtcttccg ggcagaatcc gatgcccagt actgaagcct gatgtggcga
     2581 tcgcagggtt tgcggtttgc ctttgatcaa cgccgtgccg ctgtcagcag gtttgatacc
     2641 gaagatcact tcggcggttt cggtacgtcc ggatcccagc aagccagcca gaccgacgat
     2701 ctcgccgggg cgcacttcga gatcaaacgg tgctatcgtt ccttttttgc cgtaattttt
     2761 gaacgcggca acgggtttgt cgctcaacaa tgttcgcccg gcacgctgta gcgcgtgggt
     2821 atccagctcg cgccccagca tcatttttac cagttcgatc tgcggtagct cgcgcgtttc
     2881 ccgacagcct acgaaactgc cgttgcgtaa gacggtgatc cgatcgctga cctgatagac
     2941 ctgatcgaga aagtgagtga caaagatcag gctgacgccg cgatcgcgca actgacgcat
     3001 caggccaaat aatagctcca tttcctgggt gtcgaggctg gcggtgggtt catcgaggat
     3061 cagcactttg gcggagagat cgatagcccg gcaaatggcg acgatttgct gcatcgccac
     3121 cgaaaaacgg ttaagcggtt cgcgcacgtc gagggagaaa ccgtaagatg ccatcagttc
     3181 ggtggcgcgc ttttccatct ctttacggcg tagaaggccg aaacgtttgg gttcgcgacc
     3241 tatgaataga ttatcagcaa ccgacatatt gggtagcagg ttgacttcct gatagacggt
     3301 gccgataccg agttgttgtg catgggcggt atttttcggt gagatagtct ggccttccag
     3361 ccagatggtg ccgcgatcgg cgtggtatac accagttaat gctttgatta gcgttgattt
     3421 tcccgccccg ttttcaccaa gcagcgccat gatttcgcca cggcgcaggc tgaaatcaac
     3481 gttgtctaac gctttgacgc cggggaaaaa tttacttaat ccttcggtgc ggaggatctc
     3541 ctggtgttgg tcggtcgtca tggtttcccc cggtgtaaag cgtgctccca gtaggcatga
     3601 taagacgcgg taagcgtcgc atcaggcttc agtgcaccat cgccggatgc ggcgcgagcg
     3661 ccttatccgg cctacgaatc ggcatcaggc atagcaaccc tcaatacccc atatttttct
     3721 tcttctctaa ctcttctttt gcggtatcag gcaggtaaag ggtggatttg gttaacgtca
     3781 gcttttcagg catggtgccg tcttttttgt atttctccag cgcatcgaaa gcgggtcccg
     3841 ccatgttcgg cgtcagttca acactggcgt tcgcttcgcc atccatcatc gctttgtaga
     3901 tgtccggtac accgtcaatg gaacccgtga ggatatcttt gcccggtttc aggcccgctt
     3961 ctttaattgc ctgaattgca ccaatcacca tgtcgtcgtt atgggcgtaa accatgcaga
     4021 tgtttttgcc gttgttttcc gctttgataa agctctccat gacttctttg cctttactgc
     4081 gggtgaagtc acctgactgc gagcggataa ttttgatatt tggcgcattc ttaatggctt
     4141 cggcaaagcc tttcttacgg tcaatggcga cgctggcccc aacggtgccc tgcagctcca
     4201 ccacgttgca tggtttgcca ttcacttctt ttaccagcca gtcaccgatc aacttacctt
     4261 cgaggatgtt gtcggcagta acggtggtca tatagagaga tttgtctttc acatcgatgg
     4321 aacggtcgag caagaagacc gggatttcgg catctttcgc ctcttttaat accggttccc
     4381 aacccgtcgc aaccactgga gcgataaaga tcgcatccac cccttatgca acgaaggaac
     4441 gtaccgcttt aatctggttt tcctgctttt gctgaccatc ggcaattttc aacgtaattc
     4501 cgcgtttttc ggcctcactt ttcgccacat tggtttctgc ggcgcgccag ccggattccg
     4561 atccgacctg cgaaaatcct acggttaatg gagcggccaa cgccatagac gacatggctg
     4621 ccgagactgc agagactaca agtaagcgtt tccacatagg gttgtcctcg tcggggtaat
     4681 tattattggt gaaaagatgt tcgcgaaaaa actatagaca attcgttatg taacggattg
     4741 cgttacatca cacttcaaaa aagtaaataa aacgttaatc acaagtttgt aatcgctttc
     4801 atctcactat gaaaaatgcg gctacggtta tggattttcc tgctctgtat accgtcttaa
     4861 aactggcgaa aaaggaaaat gaagacgaaa acaagcaaag acattcggcg cgagttggct
     4921 ataatacttg gcacttgttt gccacatatt tttaaaggaa acagacatga gcttactcaa
     4981 cgtccctgcg ggtaaagatc tgccggaaga catctacgtt gttattgaga tcccggctaa
     5041 cgcagatccg atcaaatacg aaatcgacaa agagagcggc gcactgttcg ttgaccgctt
     5101 catgtccacc gcgatgttct atccgtgcaa ctacggttac atcaaccaca ccctgtctct
     5161 ggacggtgac ccggttgacg tactggtccc gactccgtac ccgctgcagc ctggttctgt
     5221 gatccgttgc cgtccggttg ccgttctgaa aatgaccgac gaagccggtg aagatgcgaa
     5281 actggttgcg gttccgcaca gcaagctgag caaagaatac gatcacatta aagacgtaaa
     5341 cgatctgcct gaactgctga aagcgcaaat cgctcacttc ttcgagcact acaaagacct
     5401 cgaaaaaggc aagtgggtga aagttgaagg ttgggaaaac gcagaagccg ctaaagctga
     5461 aatcgttgct tccttcgagc gcgcaaagaa taaataagtt cttctagcgc aataaccctg
     5521 aacgccgggc ttcggttagt aagggttttt ttatgcccgc gataaataaa ctctctattc
     5581 caccatcatt attctcagcg gttgcaaggc ttgaacggta agaacaagca aacccgacca
     5641 ccattttgct gttcatagcc acttgctgga agttagccga cctcactcat actcaccgtt
     5701 caaagcgtgc ccccgtttta cccctgcaca tttgtgaacg ccagcagagt tttttatgcg
     5761 attgcagata tggaaagcgg ctcggagaga aaagcaaaag atggaaaagt tacaaaaaaa
     5821 aatcgcgaga tacatcgcat aaaacaacgc cacccgaagg aggcgtgctt ttattattac
     5881 ttatccctgt ctaaccagtc gccgctttca attagcttta atccatcgac gggtcgttga
     5941 taaacgtaca tccatgcact cccatacggc gtctgaatca actggcgcgc gtattcaccg
     6001 cccctggtgc gcaaggcatc aagttcggcc agcgtggcgt tgtcaatacg ataaacttca
     6061 ccgtgtaccg ttccgttccc cggaactgcg cctggatagt ggcccaggct atacaactgg
     6121 tagttatcga tactgaaatc gcccagtaac tgggcattgg tcatccagtg actgttgcct
     6181 tgtttgtggc gtaaactgcc gtagacaaat attcgcattg ctaaaactcg aactgataga
     6241 gcaaatccag tgcctggtct acaccagaca cggcttccag atatagctta ggcatcaggc
     6301 gataacgtaa cgtgagtgtt gctatagagt caaatatacc cacgccgtat ttcacttgca
     6361 gacctggcaa tacatagccg ctgaccacta cctgggagga gtcgcctact cccagggtgt
     6421 cgagcgctaa attgcttacg ccaaacgtct cgccgatttt acccacaatc tggccacttt
     6481 gcgcaacccc cagaccaatc agcatcgagg tcattgccgc actgtcgctc tgatcgctct
     6541 ccagcccttg tccacgtagc aaataagaca atgcagcttg ttgcgacatc gccgggtcag
     6601 agaagatctc cgctttcggt tcgtccgcca gaccagtgac gcgaactccg gcgattacat
     6661 cgtcttctgt agcatccggg ttacgaatag cttcaatatt aagatacggt tgatccggcg
     6721 gaccagagaa cagtaactca cccttacgca caatcagatc ctgaccatag gcatggaagc
     6781 gcccttcagg gatgttgatc tgcccgttca ggcccagccc ttgtttgtcc tgtacgacgt
     6841 taagatcgcc cgtcagccgc gctttcaggc caaaggcgtc aatgcgcaca ttgttgccaa
     6901 cgtggacaat caggttactg ttaatcggaa tcgacgccgt tttcggctct tccggttgca
     6961 ggttatcgtt aagcatcacc acatcgctgg agacgcctac tgcgctttcc ggcagatcgt
     7021 gcaccacgat gcgcgcccac gggacatcca cgcgaccatc gagggtaaac aggtttggtg
     7081 tagcctcgaa tacaacatct ggcgatacat ccattcgtac catcggcggc acggtgatcc
     7141 gcactttact gcctttcgcc gttactcgtg cccgccagtt ttcaatttgg ctccagtcgg
     7201 catcaccgtt caggtagatt tcaccctgct gggtccgtac tgtaccggca agcgtcgagc
     7261 gcataccgtt aaagttgacc gcaagctggc tcggctgcat atcaaacggc ataaagttgc
     7321 cgtcgatatc cacacccgta acctgaagct gaccaaacaa ctgcgggctt tgcacatcac
     7381 cacccagacg caagttggca ctcaccatcc ccgctgcttt ttccccacgg gtaaagatgg
     7441 ggtttatcat cgcaaggttg aagttacgga tattgacgtt gccaccaaga ttacggcggc
     7501 cttgcggatc ggtcacctgc acctgtccat caaactggcc gttattggtc aggcggatgg
     7561 tccagcccaa ttcggcacgg ttgttacgca attccgccgt cagattcagt gtctgaaacg
     7621 ccaccggcag cgccgcatcg ttgacggttt gcgttacctg cacgttacgc cccgaaaggg
     7681 tgatactgcc ctgcggcagc ccctctttcg tggtgtccca ggcaacatcc gctttacccg
     7741 tgaagatacc gctggcctga gtggtttctg gcataaacgg tttcagcatg gcgaggtcga
     7801 agcggttgag attcaccacc gcacgccctt cggcccccgc atcgatagtt tgcggcacgc
     7861 acagttccgc attcgggtta agccagcagt gtggcccgat gctgattttt tgctccttat
     7921 tgcggtaatc cagcgcaata tcgcgggtca gcgaccacgg gccgaccggc gtctggaagc
     7981 gggtattgct aagagttccc ttccagcgtt cttctttgcg atcaaaactt cctgccagat
     8041 taagctgccc ggagacaggc tcgccctgaa tccgcaactg tagctcgtgc tgcttttcgc
     8101 tgcctttggc attcagggtg acgaggttga tatttacatc cggctgcgaa atttgctcaa
     8161 cgcgtacgtc gagtttcccg gcgatctgat cggtggattt aatgtcgcct tccacgcgaa
     8221 cctgcgccac ggaaagttcc tgccagcgca ggccgcgcgc ggtgatatct gccagtagtt
     8281 gtggcgcttc caccgtgccg cgtactttca ccagcccttt cgctgtaccg ccaagccccg
     8341 gcagcgcgtt atccagcccc ggcgcgttga tggtggcatc aagattgaga tcttttaccc
     8401 ccagctcgcc tttcacttcg gcactgtttg gcccgagttc cagatgaagc ccagggatca
     8461 tccactgcat ataactgttg cctttcagcg tgccgtcaac gttcactttg ttctgtttga
     8521 cgttaccggt cagcttcagt tctggcacct ccatctgcca ggtgccgccg tacaggctac
     8581 cgcgggtttt aatcaagcca ttgagtttcg acggccactc cgggaactct ttggcggtgt
     8641 taatgccatt aagcgttagc tcaccgcgcc aactaatggc ctgctgccag tcgagcaacg
     8701 ccttgagttc agttttccct tccagcgccg cgacggtgag tttgtcgaga ttgacctgct
     8761 gttcattacc tttggcatca agggtaatgg tggctggcgg gatctcctgt cccttcactg
     8821 ccgtacgcat agagagcgtg taatcggtca ttttgccggt aagtttcagt ttcagatcat
     8881 ccgcctgata ctgcttctca ccagtgaacg gccagtaaag ctgtttgctg ttcacttcca
     8941 cgttgagtgg caatccggct tccgccagtc gcgtctgggc gcgtaaatcc atatccaccg
     9001 gaccggaaag gttaacgcct atctccagct gttcgcgcag cgcgccgccc actttcagct
     9061 tcaccttttc acctttcaac ggctccacgt tcagtgtgct gttgagggtg atatccaccg
     9121 gccagttgtc tgacagctgc gccgtgccgc tggcgttgac gatcccctgg ttggaatcga
     9181 tatccagggc gtccagttta gtattgccgt caatgctgct cactttcagc agcatggtgc
     9241 gcacggtgat gtccgtgtcg cccgtcacgc gcagctgctc gcccttaaac tcttcaatgt
     9301 tcaggttaag cggcaaatgc acgtcggtca tttccggcaa taccgggcga gaaaagagat
     9361 ctttcagcgt ttcgccgagc ggcttttcct ccggctgcgg attttcaatt ttcggttcga
     9421 ccacttcttc ctgcgccact tccaccactt tcggcagagc aatcagcagg cctttcagcg
     9481 acgtcggttt cagggtcagg gttttctcct gccagttcag gccggaggtg aagtccatca
     9541 ccgacaccgt ggtgtcatca atcttgatgt tgacgttgtc cagcgccacc cgtgtcaggg
     9601 tgatgggata cggcgtggag agatccagcg gaccgctatc ttcctcttct tcaacctgtt
     9661 cagaaggagg cattttttta ctgtcgatgt tgacctgaat gtctttcagc gccaggtcat
     9721 taatacaaac gctactgttc cacaggcact caagaccgac ccccagatgc agattacccg
     9781 cttttaccgc cacgcctagc tgctcataac gaacgtcaga caaggtgaga tcgcgccagc
     9841 cgccggtgac cttgccaata tccagtcctg gcacccagcg atccgccgct ttaaatacaa
     9901 gatgcaggcc gctggtggtg cccaccagaa acgccaccga tcccagcaac agtaagataa
     9961 cgataaccac gccgaggctg atttttttcc ataaactcat aattctggcc ccagaccgat
    10021 gtaaaactgt aacccgtgtt cgtctttatc cgcgaccggt acggcaaaat cgagtttgat
    10081 tggcccgacc ggcgattccc agcgtacgcc gaccccggta ccggttttaa agtcgctgcg
    10141 gcgaatatcg cttaccgctt cgccactatc gacaaacacc gcgccccacc attttccggt
    10201 cacgttgtac tggtattcca gcgatccggt tatcaacttc gaggcccctt tcaggtcacc
    10261 gttggcgtat ttcggggcga tagatttgta tttgtagccg cgaatactgc gatcgccccc
    10321 gacgaagaaa cgcagatccg gcggtacttt gtcgaaatca ccggtttcaa tccagcccag
    10381 cgtgccgcgt gtcacaaagc gatggcgatc gtacagtgtg cggatccaga cgtgctgcgc
    10441 ctggaaaacg gagaaatcga catctgaacc ccaggccgtg ttggagtagt cgatagagta
    10501 gcgttgcgag tctccccagg ttggcatcag gccaccacga gaacgcgtgc ggctaatcat
    10561 caccccagga taaaacagca tcgtggtgtt ggtaatttca ccctgggtaa agtggtcgag
    10621 gctccagcgc aggttaatgg cacgctgcca gccgctggag agatcccagt agcgagaagc
    10681 caccagcgta gtggagtcag attcggtatc gttcaggtca gtgcgcttaa aaccgccctg
    10741 caccaaataa tattgttcca gtggattctt cagcagcggc attttatagc tgaagtcgag
    10801 ggtctgttcc ggcgcggaaa tactggtact ggtggtcaaa ctgtgaccgt atgagttcat
    10861 ccacggcttt ttccacgtcg ctttcacgcg cggtcccacg tccgtagagt aaccgacccc
    10921 ggtttcgatg gtgttttctg ttcgcggcga aaccacgccc gtcaatggta atactttcgt
    10981 ttcgcgcgct ttatcaaatt gtggagccac caccaccgag ttaaaccagc cggtagcaga
    11041 aagtcggcgg ttcagttccg ccagatcttt cgattcatac tcatcgccct ctttaaacgg
    11101 caccagattt tgcaggtatt catcgcggat ttgtgatcct tcaaaggtca catgcccaaa
    11161 gcggtaacgt tcgccactgt tataatcaat atcccagaag gctttatgca ggccgagcgc
    11221 aatgcccagc tgcgctttgg taaattcgct atcgaaataa cctttacgca acgcaatact
    11281 ggttaaggac tttttgaaat tttcataatc gccctggttc agtaccgtac caatagccgg
    11341 gcgagtatcg agcaatttca aatagtcttt atcggtccgc gcgccgccgc gcaataccac
    11401 atcggtgccg ccaattaaca ccggcacacc tggcgtgact ttggcgatca atacctgccg
    11461 ccctttcttt ggcggtggac ggagatcaaa ttcaatggtc ggctggtaat aacccagcgc
    11521 tttcagacct tcacggatgg catcatcgac gcgtgcgcga aagcgacggt ctggcgtcac
    11581 ttcatcactt tcaatcgtag aaagctgcgc acgaacgttc ttttccagct gtcccgataa
    11641 cccctcgacc tgtagacgga cgttcgcggc gacggcagat ccgcttaagc agagtaagct
    11701 tacacagcat aactgtcgga tatagcgcac attttctcct gaatatcctt ttttcctgcc
    11761 cctggaacgc cgttaaacgg cttaacaaaa atccagtaat atggattaaa aagcagacta
    11821 aaccccaaat atttcttatg ttttacttta gacctattca cggtggttat tgtgtgcaaa
    11881 tacgcctctt gttacaacct taaccccaat gaccgatttt cgggagagcg acaccatgag
    11941 tttatttgat aaaaagcatc tggtttcccc cgccgatgcc ctgcctggac gtaacacccc
    12001 gatgcccgta gccacgctgc atgcggtcaa cggtcactca atgaccaatg tacctgacgg
    12061 aatggagatt gccatttttg cgatgggttg tttctggggt gtggagcgtc tgttctggca
    12121 gttacccggc gtttacagca ccgccgcagg ctataccggc ggctatacgc caaatccgac
    12181 ttatcgggaa gtgtgctccg gtgatacggg tcatgccgaa gcggtacgca ttgtttacga
    12241 tccttccgtc atcagctatg agcagttgct acaggtattt tgggagaatc acgatcccgc
    12301 ccagggaatg cgtcagggca atgaccacgg cacgcagtat cgttcagcga tttatccgct
    12361 gaccccagaa caggatgccg cagctcgcgc cagtctggaa cgttttcagg cggcgatgct
    12421 tgccgccgat gacgatcgtc acatcaccac ggaaatcgct aacgccacac cgttttatta
    12481 tgccgaagat gaccaccagc aatatctgca taaaaaccca tatggttatt gtggaattgg
    12541 cggaattggc gtctgtctgc cgccggaagc atagcgttac gggtacaaat gtagtttgtt
    12601 gataaagtgc tctttgtcta tgcctgatgc ggcttaaacg acttaccagg cctacaaaat
    12661 cgcccaaatt caatatattg caaggactgc gtaggcctga tcggcatagc gcatcaggca
    12721 gtattgcatt tatcagcagt ctgaatttta accctctggc gactttacag taccttacgc
    12781 tatactagcc actgaaaatg ccggttcact ttcttcgaat cggctttcaa tgtgtatttc
    12841 acacaaatta atcaactttc ccttccgagg gtctggcctg aaaggtcgga taagatatgt
    12901 taaacagtat tttagtcata ctctgcttga tcgctgtaag tgcgttcttc tcgatgtccg
    12961 agatctcgct tgccgcctca cgcaaaatca aacttaaact gctggctgat gaaggcaata
    13021 taaatgccca acgcgttctg aatatgcagg aaaatcccgg catgttcttt accgtggtcc
    13081 aaatcggtct gaacgcagtg gcgattctcg gcggtatcgt cggtgatgcg gcattttctc
    13141 cagcttttca cagcctgttc tcccgctata tgtcggcaga gctctctgag caactgagct
    13201 ttattctctc tttctcgtta gtgactggca tgtttatcct gtttgcggat ttaaccccga
    13261 aacgcatcgg tatgattgcg ccagaagcgg tggctttgcg tatcatcaac ccgatgcgct
    13321 tctgcctgta cgtttgcacc ccgctggtgt ggttcttcaa cggcctggcg aacatgatct
    13381 tccgtatttt caaactgcca atggtacgta aagatgacat cacttctgat gacatctacg
    13441 cggtagtgga agccggtgcg ctggcgggcg tgttacgtaa acaggaacac gagctgattg
    13501 aaaacgtctt tgagctggaa tcccgtaccg ttccgtcttc aatgacaccg cgtgaaaacg
    13561 tgatttggtt tgatctccac gaagatgagc aaagcctgaa gaataaggtg gcggaacatc
    13621 cgcactctaa gttcctcgtc tgtaatgaag atattgacca catcatcggt tatgtcgatt
    13681 ctaaagacct gctgaaccgc gtgctggcta accaaagcct ggcactgaac agcggcgtac
    13741 aaattcgcaa cacgctgatt gtgccggata cgttaaccct ttcagaggcg ttggaaagtt
    13801 ttaaaaccgc aggtgaagac ttcgcggtga tcatgaacga gtacgcgctg gtggtgggga
    13861 tcatcaccct caacgacgtg atgaccacgc tgatgggcga tctggtcggt caggggctgg
    13921 aagagcagat tgtcgcccgt gatgagaact catggctgat tgacggcggc accccaattg
    13981 acgacgtcat gcgcgtgctg gatattgacg agttcccgca gtcgggcaac tacgaaacca
    14041 tcggcggctt tatgatgttt atgctgcgta agatcccgaa acgcaccgat tcggtgaaat
    14101 tcgccggcta caaatttgaa gtggtggata tcgataacta ccgcatcgac cagctgctgg
    14161 tgacccggat cgacagcaag gccaccgccc tttcgccaaa actgcctgac gctaaagata
    14221 aagaagaaag cgtcgcgtaa cccccagaaa catcaacggc tcctgaatca ggagccgtct
    14281 tattactgca tagcactttg gttaagccat ctctgtttgc agacgcataa cctgacggtt
    14341 aacttcggac atcactgaca aatgcagctt atccttcact tttgggataa gaatcttacc
    14401 cttatcaaat tcaaaagcgc caatgtcctt gatgtataac cgtccacgga acaggatctt
    14461 tacgtacttc gccacctgaa gtgggttgta ccgttggaaa attttcattg ttatctcctg
    14521 ctgagtatta cgcctgtgcg gtgccacaat cggcccaact attatgaggc gcaaatttta
    14581 atgcttagtg actatagact atccgggcaa tgtttccacc gcgtataact ttttttacct
    14641 aaaggttaca attattcaga attatctttt taccgaagcg cggtcttcag tataagcatt
    14701 catttttcat atgatttgtg cgcttgaccg caaactggca tcacacttgc gggaaattcg
    14761 ataaatagca catatgatta aaactcagac ccaagtggtc ggatcacctg catatcataa
    14821 gaaggaaaca ccatgaccct acgcaagatt ctggcactca cctgcctgct gttgccgatg
    14881 atggcttccg cacatcagtt cgaaatcggt cagcgagtgc cgccgattgg cattaccgat
    14941 cggggcgagt tggtgcttga taaagatcag tttagctaca aaacctggaa cagcgcgcag
    15001 ttagtgggaa aagtgcgagt actgcaacat attgctggtc gcacctctgc aaaagagaaa
    15061 aacgcgacgc tgattgaagc gattaaatca gcgaagttac cgcacgatcg ttaccagacc
    15121 accaccattg ttaacaccga cgacgcgatt ccgggttccg gcatgtttgt gcgcagcagt
    15181 ctggagagta ataaaaagct ttatccctgg tcgcagttta ttgtcgatag caatggcgtc
    15241 gcgcgcggtg cctggcagct ggatgaagaa agttccgctg tagtggtgct ggataaagac
    15301 ggtcgcgtgc aatgggccaa agacggggcg ctcacaccgg aagaggtgca gcaagtgatg
    15361 gacctgctgc aaaagttgct caaataatgg caataacata aattctggaa aaataatttt
    15421 cccctatcgt tatctatggg ataggggaat taagtctctg agttcttaca ccgaactaat
    15481 actggtaacg ctagttgctc ttttttaaac gcctcaatgg tcgatttata tttctcgcaa
    15541 atcttttctt tggtaatatt actatctgcg gccagattcg cacttactat cgcatccact
    15601 tgttcggcta attcgacttg ttggcaacct gcattaacca gaaaattata aataagagag
    15661 taatcttcat cctccaaaac aacagaggaa ccattacgaa aacgttcatc ccctccatag
    15721 attttaagcg caaagcccaa agattctatg actatggcaa aattatcatc aattattttt
    15781 tcgtaaatat accatccatc gcattcttta attccaccgc ctggtatttg gagttcaaat
    15841 ttaccccgtt ttaatttgct tactacatcc tccaataagg cataaccgaa agatacgtca
    15901 ccaaacgcct ggcgaatata acctttaatc ctttttggta tgtcagactg ttttttctgc
    15961 aaaatatcac aggcatacaa tcttctgttt tgttctaacg gaaactcgcg gtttttgaaa
    16021 tccatgaatg agttatactc atttattgtt atgggaattt cttcgtattc aaacccactg
    16081 cctcgcaatg ttattgtata ttcagcaccg ttatctttaa ggctgaaact gataagaaaa
    16141 ttatccatgg cattaacagt ttcatttttt gatgaaagca cttgataact atttttatca
    16201 atctgtgttg cgcaacgaaa ggactcccgc aaagttaaca gactatcata attcgaaacc
    16261 caatcttttt tcttaaatgc atgctgaata ttattaaaaa aatcgtttga atgtgttttt
    16321 gtatgcgatg actttacaaa taactccacc gcaccaataa aattattgtg tctgatcctg
    16381 ggtaacatat ggctacctta ttttatttgg ttattttatg tcgccattaa atatctcatt
    16441 atagaagtaa actatcaaaa aacgctcatt attaaaatgt aacttactag caattgaatt
    16501 tttgcacttt aaatacaatc tctttttatt tataactttt taggttggga taaatcagga
    16561 tggcagaatc aggaaataca ctgtttctgc catctgaatt tagtaaatgg acactctgaa
    16621 ccccggattc aggaacgact cgcgcggagt gtaatccagc ggtttaccct gccagtcgtg
    16681 aacgtgcgct ccggcagctg cagccacagc atgtccggcg gcggtgtccc aaatattcgt
    16741 tggtccgaag cgcgggtaca gctgcgcctg tccttccgcc accaggcaga atttcagcga
    16801 agagccgatg gacgtggtct gatgttcgcc aagctgttgc agatactctt tcagctccgc
    16861 atctgcatgg gaacggctga taaccaccag cggcgggcgc gcatcgcgga cctgaatcag
    16921 cttgcgcaca ccgcactctt ctttccaggc cttgccttct gccgcgctgt acattacgtt
    16981 cattaccggc gcatacacca cacctaaaat cggtttgcca tggtcaatga gcgcaatgtt
    17041 aacggtgaat tcgccattac gtttaataaa ctctttagta ccatccagcg ggtctaccag
    17101 ccagtaacgc tgccagtgct gacggacttc ccaaccggga ggatcttctt cagaaaggac
    17161 cggaatatcc ggtgccagcg tacgtaaacc gtccatgata acggtgtgag cggcaatatc
    17221 cgctgccgtt accggagaat tgtccgcttt gctgacgacg tccatcggtt tcgtcccgtc
    17281 gtagacctgc ataatggcat cgcctgcatt ccgtgcaagc tggcatactt gatctaacat
    17341 ttctccacct cgtctctgtg agcggtgtta acttattgtt ttacttatac cctatcgtta
    17401 atgaatgcgc caactgtgat agtgtcatca ttttcaaagc gtaaaattgt ggcattcttc
    17461 actgttctat aagtaaggcg tttattctcc cttttctttc gtattcccga tgataaaagg
    17521 atgtccctga tgattaagtt tagcgcaacg ctcctggcca cgctgattgc cgccagtgtg
    17581 aatgcagcga cggtcgatct gcgtatcatg gaaaccactg atctgcatag caacatgatg
    17641 gatttcgatt attacaaaga caccgccacg gaaaaattcg gactggtacg tacggcaagc
    17701 ctgattaacg atgcccgcaa tgaagtgaaa aacagcgtac tggtcgataa cggcgatttg
    17761 attcagggga gtccgctggc cgattacatg tcggcgaaag gattaaaagc aggtgatgtt
    17821 catccggttt ataaggcgct gaatacgctg gattatacgg tcggtacact cggcaatcat
    17881 gaatttaact acggtctgga ttacctgaaa aatgcgctgg cgggagcgaa attcccttat
    17941 gtaaatgcca acgtcattga cgccagaacc aaacagccaa tgtttacacc gtatttaatt
    18001 aaagataccg aagtggtcga taaagacgga aaaaaacaga cgctgaagat tggctatatt
    18061 ggcgtcgtgc cgccgcaaat catgggctgg gataaagcta atttatccgg aaaagtgacg
    18121 gtgaatgata ttaccgaaac cgtgcgcaaa tacgtgcctg aaatgcgcga gaaaggtgcc
    18181 gatgtcattg tcgttctggc gcattccggg ctgtctgccg atccgtataa agtaatggcg
    18241 gaaaactcag tttattacct cagtgaaatt ccgggcgtta acgccattat gtttggccat
    18301 gctcacgccg ttttcccggg taaagatttt gctgatatcg aaggggctga tatcaccaaa
    18361 ggcacgctga atggtgttcc ggcggtaatg ccgggcatgt ggggcgatca tcttggggtg
    18421 gtcgacttac aactcagtaa tgacagcggt aaatggcagg tgacgcaggc gaaagcggaa
    18481 gcacggccga tttacgacat cgccaataaa aaatccctcg cggcggaaga cagcaagctg
    18541 gtagaaacac tcaaagccga tcacgatgcc acacgccagt tcgtcagcaa gccaatcggt
    18601 aaatctgccg acaatatgta tagctatctg gcgctggtgc aggacgatcc gaccgtgcaa
    18661 gtcgtgaaca acgcgcaaaa agcgtatgtc gaacattaca ttcagggcga tccggatctg
    18721 gcaaaactgc cggtgctttc agctgccgca ccgtttaaag ttggtggtcg caaaaatgac
    18781 ccggcaagct atgtggaggt ggaaaaaggc cagttgacct tccgtaatgc cgccgatctt
    18841 tatctctacc ccaatacgct gattgtagtg aaagccagcg gtaaagaggt gaaagagtgg
    18901 ctggagtgct ccgccggaca gtttaaccag attgctccta acagcacgaa accacagtca
    18961 ctcatcaact gggatggttt ccgcacttat aactttgatg ttattgatgg tgtgaattat
    19021 cagattgatg ttacccaacc cgcccgctat gacggcgagt gccagatgat taatgccaat
    19081 gcagagcgga ttaagaacct gacctttaat ggcaagccga ttgatccgaa cgctatgttc
    19141 ctggttgcca ccaataacta tcgcgcttac ggcggcaaat ttgccggtac gggcgacagc
    19201 catatcgctt ttgcttcacc ggatgagaac cgctcggtgc tggcagcgtg gattgctgat
    19261 gagtcgaaac gtgcggggga aattcatccg gcggcagata acaactggcg tttagcaccg
    19321 atagctgccg ataagaaact ggatatccgt ttcgaaactt ccccgtcaga taaagccgca
    19381 gcgtttatta aagagaaagg gcagtatccg atgaataaag tcgcgaccga tgatatcggg
    19441 tttgcgattt atcaggtgga tttgagtaag taaaacactt ctttttcggc ctataaatca
    19501 accgcatccg gcatttattg gcggatgcga tgctgccgta tcttatccac cctacaagcc
    19561 atgaaccgta gaccggataa gcgcagcgca tccggcagtt atgccgcacg ttcatcccgc
    19621 accgccaaca cctcgggcaa attcaactca atccagtccg ccagtgcagc aaccttttcg
    19681 ctcacctgct cgcccagcgg cgtgaggcta tattccacat gcggcggcac caccggatac
    19741 gcgatacggt taagaaaacc atcctgttct aacgcctgta acgactgcgc aagcatcttt
    19801 tcactcaccc caccgatttt gcgccgcagg tcgctaaagc gatgagtacc ttcgcgtagc
    19861 gccaccagaa tcaacacccc ccaacggctg gtgacgtgtt tcaacacctc gcgcgacggg
    19921 cactgttccg caaagagatt accctctttc agttgttgtg acaggctaac ctgactcatt
    19981 tcatacttac ctttttgcac gtacttacta aaagtaagtt taggtgttag cgtatttaaa
    20041 cacaagacaa aacgatggag acttcccatg atcgctatta ctggtgccac tggccaactt
    20101 ggtcactata ttattgaatc cttgatgaaa acggttcctg ccagccaaat agtggctatc
    20161 gttcgtaatc cggcaaaagc ccaggccctg gcagcacaag gcattaccgt gcgtcaggct
    20221 gactacagcg atgaagccgc actgacatct gcacttcagg gagtggaaaa actactgctg
    20281 atctcttcca gcgaagtggg tcaacgtgcc ccgcagcatc gtaatgttat taatgccgca
    20341 aaggcggctg gcgtgaaatt tatcgcttat accagcctgc tacatgcaga tacctccccg
    20401 ctcggcctcg ccgatgagca catcgagacg gagaaaatgc tggctgattc tggcatcgtt
    20461 tacaccctgc tgcgcaacgg ctggtacagc gaaaactacc tcgccagcgc cccggcagca
    20521 ctggaacacg gcgtatttat cggtgcggct ggcgatggca aaatcgcctc agcaacgcgg
    20581 gcagattatg cgacagctgc ggcacgcgtg attagcgaag ccggtcacga aggcaaggtt
    20641 tacgaactgg cgggcgatag tgcctggacg ttgacacagt tagcggcaga gctgaccaaa
    20701 cagagcggca aacaggttac ctatcaaaat ctgagcgaag ccgatttcgc cgcggcactg
    20761 aaaagcgtcg gactgcccga cggactggcg gatatgctgg cggattctga cgttggcgca
    20821 tcgaaaggtg gtctgtttga taacagcaaa acgcttagca aattgattgg ctgcccaacg
    20881 acaacgttag ccgaaagcgt aagccatctt tttaatgtta ataactagtt aattaaagtg
    20941 gcatcctccc gcatcctctc tgataatgac gggatgccgg gagcaatcat gtctgcttcc
    21001 tgaacgtttc ttctgacaga ccaatggatg ccagtaatga ttagcggcgt gctgtacgcc
    21061 ctgttagcag ggttgatgtg ggggcttatt tttgtcgggc cgttgatcgt gccggaatac
    21121 ccggcgatgt tgcagtcgat ggggcgttat ctggcgttag ggttaattgc gctacccatt
    21181 gcctggctgg gacgcgtgcg tctgcgtcag ttggcgcgtc gggactggct taccgccttg
    21241 atgctcacta tgatgggcaa cctcatttat tacttctgcc ttgccagtgc cattcaacgt
    21301 actggcgcgc ctgtttccac gatgattatc ggcaccctgc cggtggtgat tcctgtcttt
    21361 gccaatctgc tttatagcca gcgcgacggc aaactcgcgt ggggaaaact cgccccggca
    21421 ctgatttgta ttggcatcgg cctggcgagt gtgaatattg ctgagttaaa ccacggactc
    21481 cccgattttg actgggcacg ttatacctct ggcatcgtgc tagcgttagt ttccgtggtc
    21541 tgctgggcat ggtatgccct gcgcaacgcc cgctggctgc gggaaaatcc cgacaaacat
    21601 ccgatgatgt gggcgacggc gcaggcgctg gtcacgctgc cggtttctct catcggctat
    21661 ctcgtcgcct gttactggct gaatatacaa acgccggact tctccttacc ttttggcccc
    21721 cgtccgctgg tgtttattag tctgatggtt gcgatagccg tgctttgctc atgggttggc
    21781 gcactctgct ggaacgtcgc cagccagcga ttaccgacag tgattctcgg gccgctgatt
    21841 gttttcgaaa cgctggcagg tttgctgtac acctttttac tccgccagca aatgccgccg
    21901 ctaatgacgc tgagcggtat cgcgctgtta gtgattggcg tggtcatcgc ggtcagagca
    21961 aaaccagaaa aacctttaac tgaatctgtc tcagaaagtt gacatgctgg cagtgagtta
    22021 aataagcctc tgctacgtaa gggttatagc ttttgcctta aaggtgtatt taaaatacat
    22081 cttatcttat taagaatgag gtatcagcta tggcttatcg cgaccaacct ttaggtgaac
    22141 tggcgctctc tatccctcgc gcttcggctc tgtttcgtaa atatgatatg gattactgct
    22201 gtggcggtaa gcagacgctg gcgcgcgcgg cggcacgtaa agaactggat gttgaggtca
    22261 ttgaagctga actggcaaag cttgctgaac aaccgattga gaaagactgg cgtagcgccc
    22321 cgctggcaga aatcattgac catatcatcg tgcgctacca cgatcgtcac cgcgagcagc
    22381 tgccggagct gatcctgcaa gcgactaaag tcgagcgcgt tcacgccgac aaaccgagcg
    22441 tgccaaaagg gctgacaaaa tacctgacca tgctgcatga agagctttcc agccacatga
    22501 tgaaagaaga gcagatcctc ttcccgatga tcaaacaagg catgggcagc caggcaatgg
    22561 ggccaatcag cgtaatggaa agcgagcacg atgaagcggg cgaactgctg gaagtgatca
    22621 aacacactac caataacgtc acaccgccgc cagaagcctg caccacctgg aaagcgatgt
    22681 ataacggcat taatgaactg attgatgacc tgatggatca catcagtctg gaaaacaatg
    22741 tactgttccc acgcgcccta gcgggtgagt gatctaaaag ctggatggca ttgcgccatc
    22801 cagcatgata atgcggttat ttccgcagtt cagcagcccg cttcttacca ataaacagcc
    22861 agcccaaccc cagcgcgata aaccacagcg gggtaaccag cagcgcctgg cgagtgtcat
    22921 cttccagtgt cagcaacacg accacgaaca caaagaacgc catacatacc cagcacatca
    22981 gcttgccgag cggcatcttg tagatcgact tttcatgcag atgaggacgc tgtttgcggt
    23041 acaccaggta agagcaaagg ataatcgtcc agacgaacat aaacagaatc gcggaaacgg
    23101 ttgtaatcat cgtgaacgcg ccaatcacgc taggattcac atacaacatc accacgccgc
    23161 cgagcagaca gatacacgag aaggtcaacc ctttcgcggg tactgcgcgc ttagaaagtt
    23221 tagcgaacgc tttcggcgca acaccttcct gcgccagacc aaacagcata cggctggtag
    23281 agaagacacc gctgttagcg gaagacgccg cagaggtcag caccacaaag ttgatcacgc
    23341 tggcagccgc aggcaaacct accaacacaa acagctcaac gaacgggctt ttctccggga
    23401 ctaccgaact ccacggtgtc acggacataa tcacaatcag cgcgaagacg tagaacataa
    23461 tgatacggat cggaatggag ttaatcgcgc gtggcagtga tttctccgga tctttggttt
    23521 ccgcagctgt tgtacctacc agctcaatcc ccacgaaagc gaaaaccgct atctggaatc
    23581 cggcaaagaa gccacttaaa cctttcggga accagccgcc gtcattccac aaatgcgcga
    23641 atgacgcttc cacaccggtc ggtgactgaa agtgcatcgc caccatgacc aggccgacga
    23701 caatcagcga cacgatggcg acgattttga tcatcgcaaa ccagaactcc atctcaccga
    23761 acattttcac ggtggcaagg ttgagcgtca gcagcagcac tatcaccgcc agcgaggcga
    23821 cccagtcgga gagatcgggg aaccagaact gagcataagc cgtgatcgcc accacgtctg
    23881 ccataccggt tacaacccag cagaaccagt aagtccagcc ggtgaaatat cccgcccacg
    23941 gccccagtaa atcggaagcg aagtcactaa aagatttgta ttccagattc gaaagcagca
    24001 attcccccat tgcccgcatc acgaaaaaga gcataaaacc aatgatcata taaacgaaaa
    24061 tgatcgacgg cccggcaagg ctaatcgttt tgccggaccc cataaacaac ccagtaccaa
    24121 tggcacctcc aatggcaata agctgaacat gtcggtttgt gagattgcgc cgtagcgact
    24181 gttcagccgg agcctgatca tcggcaacga cttttacctg atctaccatg ttttttcttc
    24241 ctgtacctgt ctgtgttgtt caggctctac ggcctttagt cagtctatga tacgacgaat
    24301 ctgtatcggg actcatcgat attaggtaac aatacgcggg atgaatacta agatttagat
    24361 ctaatgttaa ttttatgttt aaagtgagtg tcatatcacg gtgataacgc gaaacagctc
    24421 acaaaaatac acgcttacgg ggtctttgca acacaaaata gcgccgtttg gcaaactgaa
    24481 gggtttattg ctgaatgcct gctcccctct ggttcgaggg gaacagaatg cgctgcttag
    24541 aggatttcca gcagttcgac ttcaaacacc agggtgctga acggagggat ggatgcacct
    24601 gcgccgcgct cgccatatgc cagttcctgc gggatagtca gttcccattt agaacctacc
    24661 ggcatcagag tcagtgcttc aatccagcca gggatcacgc cattaaccgg gaattcagcg
    24721 ggttcaccac gagcaacgga gctgtcaaac acggtgccgt cgatcagttt accggtgtaa
    24781 tgaacacgaa cgcggtcggt acgtgccgga attgcgcctt caccctggtt gatcacgcgg
    24841 aattgcaggc cagattcggt gctattcaca ccttcttttt tggcgttttc ttccaggtat
    24901 ttcacgcctt cagcagccat cgcctggaaa cgctgacgac gaacggcatc ggcgcgttcg
    24961 tggatttcac gcagcgcgcg atgcaccaca tcaaccggca cagccggatg tttgccttcc
    25021 agcgcatcgg caatacctgc aaccagtgct tctggcagca gcccttccag gccagattca
    25081 ctcagttgtt gccctacctg caaaccaatg ccgtagcttg cttgcgcttc gatggtgtca
    25141 aaagttgggg tggtcatcgt atttcctttc ttagggtata aaagtagcgg gcagcatatc
    25201 agccacactt ctttgggtaa aactttgtcg caggaatgat gacaaatccc ggggaaaaag
    25261 aaacaatagc aatatcccgt atgaatataa taaaggcggg tttaccacgg acatcatagc
    25321 gttagcttat tcataggcta tgattgagga acaagacgtg gagcaggagg aaaccatgcc
    25381 cgggcgcttt gaactaaaac caaccctgga gaaagtctgg cacgcgccgg acaattttcg
    25441 cttcatggac ccgctgccgc cgatgcatcg tcgggggatc attattgccg ccattgtatt
    25501 ggtggtcgga tttctgcttc catctgatga tacgcccaac gcgtcggtgg tgacgcgtga
    25561 agcgcagtta gatattcagt cgcaatcaca accgccgaca gaagaacagc cgcaaactac
    25621 ccaaacgcaa ccattccagc cagatagcgg aattgataat caatggcgct cttaccgtgt
    25681 cgaacccggt aaaacgatgg cgcagctgtt ccgcgaccac ggactgcccg ctaccgatgt
    25741 ctatgcaatg gcgcaggtag aaggcgcggg taagccgctg agtaatttgc aaaatgggca
    25801 gatggtgaaa atccgccaga acgccagcgg cgtggtgacg ggcttaacca ttgatacggg
    25861 gaataatcaa caggtcctgt ttacccgcca gccggatggc agttttattc gtgcgcggta
    25921 gatgaagggt caaccaaaag ccagctaagg attgtctctt tgatgacctt acgtctggct
    25981 tccagtgcct ggggggaaac catactgata tgataaacta ggccaagcgt atgctggttg
    26041 atcaaataat atcccccaag cgcagcaata ttaatattca cctgcaatgg gtcgatacct
    26101 ggcttaaaaa tattgtattt cttcccttca tccaataagg attccattaa ctgcaagtga
    26161 gcatggttaa tttctaagag acgctgtgat tttgcataat gcacaccttt actttgattt
    26221 tcactatgca caatttttaa aaaccaagga ttagcaatat aataatccca tgtaaaatca
    26281 ataattttct caatggcttt ttgtggcgga agatctgaga aatcaagggc cttttctttt
    26341 tgtcgaatat cgttccaggt atattccagc acttcgataa ataaattttc tttattacca
    26401 aagtggtgat aaaccgtctg tttactacac cctgctgcct ttacaatatt atctactctg
    26461 gccccttcat aaccatattc ggcaaattca ttaaccgcac atagcagcaa tttttccttc
    26521 aggctctgta cgttgctggt atcaatatac ataacgcttc cccacgacct cttcgtggtg
    26581 actatcataa cctgatttga tttcagcttc agaaaaaagg gcaaaaactt gtactttcat
    26641 caggttatca aatgtcctgt gatcgccatc gcatatcaac caactagatg gtttatcttt
    26701 ttatcctcca gtcgcaagct aatcatcacc aaggaaacaa aattcaaaaa agagagctat
    26761 tgtatgaaat ccactcgtcc tgttgccgtg attaccggag ccactcgcgg tattggaaag
    26821 ggctgtgcgc aggagcttgc tcgtggcggg tttaacctgc tgattaacga tcgtcccgat
    26881 gccgacagcg tagaaaaact gcatatcaca caacaagaat gtttggcgga aggcgtggaa
    26941 gtgatttgct tccctgctga tgtcggtgat ctatctctgc atgaggagat gctcgacgcg
    27001 gcgcaaaacc agtgggggcg gctggactgt ctgctcaata acgcggggat ttcagtgaaa
    27061 aaacgcggtg acctcctcga cctcgaacca gacagcttcg atcaaaacat tgccattaac
    27121 acccgcgcgc cgttcttcct tgctcaggca ttcagtaagc gcttactggc acagccgaaa
    27181 ccggaagcgg agctgccgca ccgctcgatt attttcgtca gttccatcaa cgccatcatg
    27241 ctggcgatga atcgcggcga atacaccatt gccaaaaccg ccgtttctgc cgctgctcgt
    27301 ctgtttgctg ctcgcctttg taatgaacaa attggcgttt atgaagtgcg ccctggtctc
    27361 atcaaaactg atatgactat tccggcaacg gcttattacg acgaactgat tgccaaaggt
    27421 ctggtgccgt ggggacgttg gggttatccg gctgatattg cttccaccgt ccgcgcgatg
    27481 gcggaaggta aactgattta cacctgtggt caggcggtcg caatcgacgg cggcttaagt
    27541 atgccgcgct tctgaggctg aatcatgaga gatctgttac aacgcccgga tttattcagt
    27601 atcaataccg cgacactggg ctataaaacg ccgctacccg ccattattga tgcctgcgcc
    27661 gcacgcggta ttggcgcaat tgccccctgg cgcagggagt tgcagggtga agatctccag
    27721 caaatcaccc gccagcttgc cgccagtaac atgagcgttt ccggtttgtg ccgtagcact
    27781 tactacaccg cgccgacact ggcggaacgc aaactggcga ttgatgataa ccgccgggcg
    27841 ctggacgacg cagcggtgct taacgccgcc tgttatatgc aggtggttgg cggtctgcca
    27901 acgggcacta aagatctata tgaagcgcga gaacaagtga aacaagggat tcgccagttg
    27961 ctgccacatt cgaaagatgt cggcgtgcca attgcgctgg agccactgca cccgatgaca
    28021 gctgccgatc gctcttgcct gtgcacgtta cgtcaggcgc tggactggtg tgatgaactc
    28081 gatcccgatg gtgagtttgg cctcggcgtg gcggtggatg tttatcacgt ctggtgggat
    28141 ccggatctc
//
LOCUS       DAJTRC010000054        26784 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.054, whole genome shotgun sequence.
ACCESSION   DAJTRC010000054 DAJTRC010000000
VERSION     DAJTRC010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26784
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.054"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            282..1580
                     /gene="kgtP"
                     /locus_tag="OO357_002392"
     CDS             282..1580
                     /gene="kgtP"
                     /locus_tag="OO357_002392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate permease"
                     /protein_id="HCR5887309.1"
                     /translation="MAESTVTADSKLTSSDTRRRIWAIVGASSGNLVEWFDFYVYSFC
                     SLYFAHIFFPSGNTTTQLLQTAGVFAAGFLMRPIGGWLFGRIADKHGRKKSMLLSVCM
                     MCFGSLVIACLPGYETIGTWAPALLLLARLFQGLSVGGEYGTSATYMSEVAVEGRKGF
                     YASFQYVTLIGGQLLALLVVVVLQHTMEDAALREWGWRIPFALGAVLAVVALWLRRQL
                     DETSQQETRALKEAGSLKGLWRNRRAFIMVLGFTAAGSLCFYTFTTYMQKYLVNTAGM
                     HANVASGIMTAALFVFMLIQPLIGALSDKIGRRTSMLCFGSLAAIFTVPILSALQNVS
                     SPYAAFGLVMCALLIVSFYTSISGILKAEMFPAQVRALGVGLSYAVANAIFGGSAEYV
                     ALSLKSIGMETAFFWYVTLMAVVAFLVSLMLHRKGKGMRL"
     gene            complement(1577..1900)
                     /locus_tag="OO357_002393"
     CDS             complement(1577..1900)
                     /locus_tag="OO357_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417081.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiM family lipoprotein"
                     /protein_id="HCR5887310.1"
                     /translation="MRILFVCSLLLLSECSHMDNDSWSGQDKAQHFIASAILSAAGNE
                     YSQHQGMSRDRSAMFGLMFSVSLGASKELWDSRPEGSGWSWKDLAWDVAGASTGYTVW
                     QLTRH"
     gene            complement(1946..3301)
                     /gene="pssA"
                     /locus_tag="OO357_002394"
     CDS             complement(1946..3301)
                     /gene="pssA"
                     /locus_tag="OO357_002394"
                     /EC_number="2.7.8.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--serine
                     O-phosphatidyltransferase"
                     /protein_id="HCR5887311.1"
                     /translation="MLSKFKRNKHQQHLAQLPKISQSVDDVDFFYAPADFRETLLEKI
                     ASAKQRICIVALYLEQDDGGKGILNALYEAKRQRPELDVRVLVDWHRAQRGRIGAAAS
                     NTNADWYCRMAQENPGVDVPVYGVPINTREALGVLHFKGFIIDDSVLYSGASLNDVYL
                     HQHDKYRYDRYHLIRNRKMSDIMFEWVTQNIMNGRGVNRLDDVNRPKSPEIKNDIRLF
                     RQELRDAAYHFQGDADNDQLSVTPLVGLGKSSLLNKTIFHLMPCAEQKLTICTPYFNL
                     PAILVRNIIQLLREGKKVEIIVGDKTANDFYIPEDEPFKIIGALPYLYEINLRRFLSR
                     LQYYVNTDQLVVRLWKDDDNTYHLKGMWVDDKWMLITGNNLNPRAWRLDLENAILIHD
                     PQLELAPQREKELELIRERTTIVKHYRDLQSIADYPVKVRKLIRRLRRIRIDRLISRI
                     L"
     gene            complement(3415..6075)
                     /gene="pat"
                     /locus_tag="OO357_002395"
     CDS             complement(3415..6075)
                     /gene="pat"
                     /locus_tag="OO357_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein lysine acetyltransferase"
                     /protein_id="HCR5887312.1"
                     /translation="MSQRGLEALLRPKSIAVIGASMKPNRAGYLMMRNLLAGGFNGPV
                     LPVTPAWKAVLGVLAWPDIASLPFTPDLAVLCTNASRNLALLEELGEKGCKTCIILSA
                     PASQHEDLRACALRHNMRLLGPNSLGLLAPWQGLNASFSPVPIKRGKLAFISQSAAVS
                     NTILDWAQQREMGFSYFIALGDSLDIDVDELLDYLARDSKTSAILLYLEQLSDARRFV
                     SAARSASRNKPILVIKSGRSPAAQRLLNTTAGMDPAWDAAIQRAGLLRVQDTHELFSA
                     VETLSHMRPLRGDRLMIISNGAAPAALALDALWSRNGKLATLSEETCQKLRDALPEHV
                     AVSNPLDLRDDASSEHYVKTLDILLHSQDFDALMVIHSPSAAAPATESAQVLIEAVKH
                     HPRSKYVSLLTNWCGEHSSQEARRLFSEAGLPTYRTPEGTITAFMHMVEYRRNQKQLR
                     ETPALPSNLTSNTAEAHLLLQQAIAEGATSLDTHEVQPILQAYGMNTLPTWIASDSTE
                     AVHIAEQIGYPVALKLRSPDIPHKSEVQGVMLYLRTANEVQQAANAIFDRVKMAWPQA
                     RVHGLLVQSMANRAGAQELRVVVEHDPVFGPLIMLGEGGVEWRPEDQAVVALPPLNMN
                     LARYLVIQGIKSKKIRARSALRPLDVAGLSQLLVQVSNLIVDCPEIQRLDIHPLLASG
                     SEFTALDVTLDISPFEGDNESRLAVRPYPHQLEEWVELKNGERCLFRPILPEDEPQLQ
                     QFISRVTKEDLYYRYFSEINEFTHEDLANMTQIDYDREMAFVAVRRIDQTEEILGVTR
                     AISDPDNIDAEFAVLVRSDLKGLGLGRRLMEKLITYTRNHGLQRLNGITMPNNRGMVA
                     LARKLGFNVDIQLEEGIVGLTLNLARREES"
     gene            complement(6107..6805)
                     /gene="tapT"
                     /locus_tag="OO357_002396"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
     CDS             complement(6107..6805)
                     /gene="tapT"
                     /locus_tag="OO357_002396"
                     /gene_synonym="tuaA"
                     /gene_synonym="yfiP"
                     /EC_number="2.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311476.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-uridine aminocarboxypropyltransferase"
                     /protein_id="HCR5887313.1"
                     /translation="MTENAVLQLRAERIARATRPFLARGNRVRRCQRCLLPEKLCLCS
                     TITPAQAKSRFCLLMFDTEPMKPSNTGRLIADILPDTVAFQWSRTEPSQDLLDLVQNP
                     DYQPMVVFPASYADEQREVIFTPPAGKPPLFIMLDGTWPEARKMFRKSPYLDNLPVIS
                     VDLSRLSAYRLREAQAAGQYCTAEVAIALLDMAGDTGAAAGLGEHFTRFKTRYLAGKT
                     QHLGSITAEQLESV"
     gene            complement(6874..7293)
                     /gene="trxC"
                     /locus_tag="OO357_002397"
     CDS             complement(6874..7293)
                     /gene="trxC"
                     /locus_tag="OO357_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin TrxC"
                     /protein_id="HCR5887314.1"
                     /translation="MNTVCTHCQAINRIPDDRIEDAAKCGRCGHDLFDGEVINATGET
                     LDKLLKDDLPVVIDFWAPWCGPCRNFAPIFEDVAQERSGKVRFVKVNTEAERELSSRF
                     GIRSIPTIMIFKNGQVVDMLNGAVPKAPFDSWLNESL"
     gene            7500..8537
                     /locus_tag="OO357_002398"
     CDS             7500..8537
                     /locus_tag="OO357_002398"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA/rRNA methyltransferase"
                     /protein_id="HCR5887315.1"
                     /translation="MNDEMKGKSGKVKVMYVRSDDDSDKRTHNPRTGKGGGRPGKSRA
                     DGGRRPARDDKQSQPRDRKWEDSPWRTVSRAPGDETPEKADHGGISGKSFIDPEVLRR
                     QRAEETRVYGENACQALFQSRPEAIVRAWFIQSVTPRFKEALRWMAANRKAYHVVDEA
                     ELTKASGTEHHGGVCFLIKKRNGTTVQQWVIQAGAQDCVLALENESNPHNLGGMMRSC
                     AHFGVKGVVVQDAALLESGAAIRTAEGGAEHVQPITGDNIVNVLDDFRQAGYTVVTTS
                     SEQGKPLFKTSLPAKMVLVLGQEYEGLPDAARDPNDLRVKIDGTGNVAGLNISVATGV
                     LLGEWWRQNKA"
     gene            complement(8585..9274)
                     /gene="ung"
                     /locus_tag="OO357_002399"
     CDS             complement(8585..9274)
                     /gene="ung"
                     /locus_tag="OO357_002399"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HCR5887316.1"
                     /translation="MANELTWHDVLAEEKQQPYFLNTLQTVASERQSGVTIYPPQKDV
                     FNAFRFTELGDVKVVILGQDPYHGPGQAHGLAFSVRPGIAIPPSLLNMYKELENTIPG
                     FTRPNHGYLESWARQGVLLLNTVLTVRAGQAHSHASLGWETFTDKVISLINQHREGVV
                     FLLWGSHAQKKGAIIDKQRHHVLKAPHPSPLSAHRGFFGCNHFVLANQWLEQRGETPI
                     DWMPVLPAECE"
     gene            9579..9962
                     /gene="grcA"
                     /locus_tag="OO357_002400"
     CDS             9579..9962
                     /gene="grcA"
                     /locus_tag="OO357_002400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007728348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autonomous glycyl radical cofactor GrcA"
                     /protein_id="HCR5887317.1"
                     /translation="MITGIQITKAANADLLNSFWLLDSEKGEARCIVAKAGYAEDEVV
                     AVSKLGDIEYREVPVEVKPEVRVEGGQHLNVNVLRRETLEDAVKHPEKYPQLTIRVSG
                     YAVRFNSLTPEQQRDVIARTFTESL"
     gene            complement(10018..10605)
                     /gene="eamB"
                     /locus_tag="OO357_002401"
     CDS             complement(10018..10605)
                     /gene="eamB"
                     /locus_tag="OO357_002401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine/O-acetylserine transporter"
                     /protein_id="HCR5887318.1"
                     /translation="MTPILLSAFWTYTLITAMTPGPNNILALSSATSHGFRQSTRVLA
                     GMSLGFLIVMLLCAGISFSLAVIDPAAVHLLSWAGAAYIVWLAWKIATSPTKEDGLQA
                     KPISFWASFALQFVNVKIILYGVTALSTFVLPQTQALSWVVGVSVLLAMIGTFGNVCW
                     ALAGHLFQRLFRQYGRQLNIVLALLLVYCAVRIFY"
     gene            10708..11589
                     /locus_tag="OO357_002402"
     CDS             10708..11589
                     /locus_tag="OO357_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005051239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5887319.1"
                     /translation="MDLRRFITLKTVVEEGSFLRASQKLCCTQSTVTFHIQQLEQEFS
                     VQLFEKIGRRMCLTREGKKLLPHIYELTRVMDTLREAAKKESDPDGELRVVSGETLLS
                     YRMPQVLQRFRQRAPKVRLSLQALNCYVIRDALLNDEADVGVFYRVGNDDALNRRELG
                     EQSLVLVASPQIADVDFTEPGRHNACSFIINEPQCVFRQIFESTLRQRRITVENTIEL
                     ISIESIKRCVAANIGVSYLPHFAVEKEIESGELIELPFGEQSQTITAMCAHYAGKAVS
                     PAMHIFIQCVEECFVVA"
     gene            complement(11624..12958)
                     /gene="srmB"
                     /locus_tag="OO357_002403"
     CDS             complement(11624..12958)
                     /gene="srmB"
                     /locus_tag="OO357_002403"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase SrmB"
                     /protein_id="HCR5887320.1"
                     /translation="MTVTTFSELELDESLLEALQDKGFTRPTAIQAAAIPPALDGRDV
                     LGSAPTGTGKTAAYLLPALQHLLDFPRKKSGPPRILILTPTRELAMQVADHARELAKH
                     THLDIATITGGVAYMNHAEVFSENQDIVVATTGRLLQYIKEENFDCRAVETLILDEAD
                     RMLDMGFAQDIEHIAGETRWRKQTLLFSATLEGDAIQDFAERLLEDPVEVSANPSTRE
                     RKKIHQWYYRADDLEHKTALLVHLLKQPEATRSIVFVRKRERVHELANWLREAGINNC
                     YLEGEMVQGKRNEAIKRLTEGRVNVLVATDVAARGIDIPDVSHVFNFDMPRSGDTYLH
                     RIGRTARAGRKGTAISLVEAHDHLLLGKVGRYIEEPIKARVIDELRPKTRAPSEKQTG
                     KPSKKVLAKRAEKKKAKEKEKPRVKKRHRDTKNIGKRRKPSGTGVPPQTTEE"
     gene            13090..13827
                     /gene="trmN"
                     /locus_tag="OO357_002404"
     CDS             13090..13827
                     /gene="trmN"
                     /locus_tag="OO357_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(1)(Val)
                     (adenine(37)-N(6))-methyltransferase TrmN"
                     /protein_id="HCR5887321.1"
                     /translation="MSQSTSVLRRNGFTFKQFFVAHDRCAMKVGTDGILLGAWAPVAG
                     VKRCLDIGAGSGLLALMLAQRTSDSVIIDAVELESEAAAQAQENINQSPWAERINVHT
                     ADIQQWITQQTVRFDLIISNPPYYQQGVECATPQREQARYTTTLDHPSLLTCAAECIT
                     EEGFFCVVLPEQIGNGFTELALSMGWHLRLRTDVAENEARLPHRVLLAFSPQAGECFS
                     DRLVIRGPDQNYSEAYTALTQAFYLFM"
     gene            complement(13812..15434)
                     /gene="nadB"
                     /locus_tag="OO357_002405"
                     /pseudo
     CDS             complement(13812..15434)
                     /gene="nadB"
                     /locus_tag="OO357_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417069.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
     gene            15690..15845
                     /gene="rseD"
                     /locus_tag="OO357_002406"
     CDS             15690..15845
                     /gene="rseD"
                     /locus_tag="OO357_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rpoE leader peptide RseD"
                     /protein_id="HCR5887322.1"
                     /translation="MIRLQHDKQKQMRYGTLQKRDTLTLCLLKLQLMEWRFDSAWKFG
                     LGRLYLG"
     gene            15842..16417
                     /gene="rpoE"
                     /locus_tag="OO357_002407"
     CDS             15842..16417
                     /gene="rpoE"
                     /locus_tag="OO357_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005070454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoE"
                     /protein_id="HCR5887323.1"
                     /translation="MSEQLTDQVLVERVQKGDQKAFNLLVVRYQHKVASLVSRYVPSG
                     DVPDVVQEAFIKAYRALDSFRGDSAFYTWLYRIAVNTAKNYLVAQGRRPPSSDVDAIE
                     AENFESGGALKEISNPENLMLSEELRQIVFRTIESLPEDLRMAITLRELDGLSYEEIA
                     AIMDCPVGTVRSRIFRAREAIDNKVQPLIRR"
     gene            16450..17100
                     /gene="rseA"
                     /locus_tag="OO357_002408"
     CDS             16450..17100
                     /gene="rseA"
                     /locus_tag="OO357_002408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708424.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma-E factor RseA"
                     /protein_id="HCR5887324.1"
                     /translation="MQKEQLSALMDGETLDSELLNELAHNPEMQKTWESYHLIRDSMR
                     GDTPEVLHFDISSRVMAAIEEEPVRQSATLIPEAQPAPHQWQKMPFWQKVRPWAAQLT
                     QMGVAACVSLAVIVGVQHYNGQSETSQQPETPVFNTLPMMGKASPVSLGVPSEATANN
                     GQQQQVQEQRRRINAMLQDYELQRRLHSEQLQFEQAQTQQAAVQVPGIQTLGTQSQ"
     gene            17100..18056
                     /gene="rseB"
                     /locus_tag="OO357_002409"
     CDS             17100..18056
                     /gene="rseB"
                     /locus_tag="OO357_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-E factor regulatory protein RseB"
                     /protein_id="HCR5887325.1"
                     /translation="MKQLWFAMSLVTGSLLFSANASATPASGALLQQMNLASQSLNYE
                     LSFISINKQGVESLRYRHARLDNRPLAQLLQMDGPRREVVQRGNEISYFEPGLEPFTL
                     NGDYIVDSLPSLIYTDFKRLSPYYDFISVGRTRIADRLCEVIRVVARDGTRYSYIVWM
                     DTESKLPMRVDLLDRDGETLEQFRVIAFNVNQDISSSMQTLAKANLPPLLSVPVGEKA
                     KFSWTPTWLPQGFSEVSSSRRPLPTMDNMPIESRLYSDGLFSFSVNVNRATPSSTDQM
                     LRTGRRTVSTSVRDNAEITIVGELPPQTAKRIAENIKFGAAQ"
     gene            18053..18532
                     /gene="rseC"
                     /locus_tag="OO357_002410"
     CDS             18053..18532
                     /gene="rseC"
                     /locus_tag="OO357_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SoxR-reducing system protein RseC"
                     /protein_id="HCR5887326.1"
                     /translation="MIKEWATVVSWQNGQALVSCDVKASCSSCASRAGCGSRVLNKLG
                     PQTTHTIVVPCDEPLVPGQKVELGIAEGSLLSSALLVYMSPLVGLFLIASLFQLLFAS
                     DVAALCGAVLGGIGGFLIARGYSRKFAARAEWQPIILSVALPPGLVRFETSSEDASQ"
     gene            18730..20529
                     /gene="lepA"
                     /locus_tag="OO357_002411"
     CDS             18730..20529
                     /gene="lepA"
                     /locus_tag="OO357_002411"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HCR5887327.1"
                     /translation="MKNIRNFSIIAHIDHGKSTLSDRIIQICGGLSDREMEAQVLDSM
                     DLERERGITIKAQSVTLDYKASDGETYQLNFIDTPGHVDFSYEVSRSLAACEGALLVV
                     DAGQGVEAQTLANCYTAMEMDLEVVPVLNKIDLPAADPERVAEEIEDIVGIDATDAVR
                     CSAKTGVGVQDVLERLVRDIPPPEGDPEGPLQALIIDSWFDNYLGVVSLIRIKNGTLR
                     KGDKVKVMSTGQTYNADRLGIFTPKQVDRTELKCGEVGWLVCAIKDIHGAPVGDTLTL
                     ARNPAEKALPGFKKVKPQVYAGLFPVSSDDYEAFRDALGKLSLNDASLFYEPESSSAL
                     GFGFRCGFLGLLHMEIIQERLEREYDLDLITTAPTVVYEVETTSREVIYVDSPSKLPA
                     VNNIYELREPIAECHMLLPQAYLGNVITLCVEKRGVQTNMVYHGNQVALTYEIPMAEV
                     VLDFFDRLKSTSRGYASLDYNFKRFQASDMVRVDVLINGERVDALALITHRDNSQNRG
                     RELVEKMKDLIPRQQFDIAIQAAIGTHIIARSTVKQLRKNVLAKCYGGDISRKKKLLQ
                     KQKEGKKRMKQIGNVELPQEAFLAILHVGKDNK"
     gene            20545..21519
                     /gene="lepB"
                     /locus_tag="OO357_002412"
     CDS             20545..21519
                     /gene="lepB"
                     /locus_tag="OO357_002412"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HCR5887328.1"
                     /translation="MANMFALILVIATLVTGILWCVDKFFFAPKRRERQAAAQAAAGD
                     SLDKATLKKVAPKPGWLETGASVFPVLAIVLIVRSFIYEPFQIPSGSMMPTLLIGDFI
                     LVEKFAYGIKDPIYQKTLIETGHPKRGDIVVFKYPEDPKLDYIKRAVGLPGDKVTYDP
                     VSKELTIQPGCSSGQACENALPVTYSNVEPSDFVQTFSRRNGGEATSGFFEVPKNETK
                     ENGIRLSERKETLGDVTHRILTVPIAQDQVGMYYQQPGQQLATWIVPPGQYFMMGDNR
                     DNSADSRYWGFVPEANLVGRATAIWMSFDKQEGEWPTGVRLSRIGGIH"
     gene            21791..22471
                     /gene="rnc"
                     /locus_tag="OO357_002413"
     CDS             21791..22471
                     /gene="rnc"
                     /locus_tag="OO357_002413"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004248457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HCR5887329.1"
                     /translation="MNPIVINRLQRKLGYTFNHQELLQQALTHRSASSKHNERLEFLG
                     DSILSYVIANALYHRFPRVDEGDMSRMRATLVRGNTLAELAREFELGECLRLGPGELK
                     SGGFRRESILADTVEALIGGVFLDSDIQTVEKLILNWYQTRLDEISPGDKQKDPKTRL
                     QEYLQGRHLPLPTYLVVQVRGEAHDQEFTIHCQVSGLSEPVVGTGSSRRKAEQAAAEQ
                     ALKKLELE"
     gene            22468..23373
                     /gene="era"
                     /locus_tag="OO357_002414"
     CDS             22468..23373
                     /gene="era"
                     /locus_tag="OO357_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HCR5887330.1"
                     /translation="MSIDKSYCGFIAIVGRPNVGKSTLLNKLLGQKISITSRKAQTTR
                     HRIVGIHTEGAYQAIYVDTPGLHMEEKRAINRLMNKAASSSIGDVELVIFVVEGTRWT
                     PDDEMVLNKLREGKAPVILAVNKVDNVQEKADLLPHLQFLASQMNFLDIVPISAETGL
                     NVDTIAAIVRKHLPEATHHFPEDYITDRSQRFMASEIIREKLMRFLGAELPYSVTVEI
                     ERFVSNERGGCDINGLILVEREGQKKMVIGNKGAKIKTIGIEARKDMQEMFEAPVHLE
                     LWVKVKSGWADDERALRSLGYVDDL"
     gene            23385..24113
                     /gene="recO"
                     /locus_tag="OO357_002415"
     CDS             23385..24113
                     /gene="recO"
                     /locus_tag="OO357_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HCR5887331.1"
                     /translation="MEGWQRAFVLHSRPWSETSLMLDVFTEESGRVRLVAKGARSKRS
                     TLKGALQPFTPLLLRFGGRGEVKTLRSAEAVSLALPLSGITLYSGLYINELLSRVLEY
                     ETRFSELFFDYLHCIQSLAGDTGTPEPALRRFELALLGHLGYGVNFTHCAGSGEPVDG
                     TMTYRYREEKGFIASVVIDNKTFTGRQLKALNAREFPDADTLRAAKRFTRMALKPYLG
                     GKPLKSRELFRQFMPKRTVKTHYE"
     gene            24125..24856
                     /gene="pdxJ"
                     /locus_tag="OO357_002416"
     CDS             24125..24856
                     /gene="pdxJ"
                     /locus_tag="OO357_002416"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708416.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HCR5887332.1"
                     /translation="MAELLLGVNIDHIATLRNARGTAYPDPVQAAFIAEQAGADGITV
                     HLREDRRHITDRDVRILRQTLDTRMNLEMAVTEEMLAIAVETKPHFCCLVPEKRQEVT
                     TEGGLDVAGQREKIRDACKRLADAGIQVSLFIDADEEQIKAAAEVGAPFIEIHTGCYA
                     DAKTDAEQAQELVRIAKAATFAASLGLKVNAGHGLTYHNVKAIAAIPEMHELNIGHAI
                     IGRAVMTGLKDAVAEMKRLMLEARG"
     gene            24856..25236
                     /locus_tag="OO357_002417"
     CDS             24856..25236
                     /locus_tag="OO357_002417"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HCR5887333.1"
                     /translation="MAILGLGTDIVEIARIEAVIARSGERLARRVLSDNEWAIWKTHH
                     QPVRFLAKRFAVKEAAAKAFGTGIRNGLAFNQFEVFNDELGKPRLRLWGEALKLAEKL
                     GVVNMHVTLADERHYACATVIIES"
     gene            25656..25736
                     /gene="shoB"
                     /locus_tag="OO357_002418"
     CDS             25656..25736
                     /gene="shoB"
                     /locus_tag="OO357_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin ShoB"
                     /protein_id="HCR5887334.1"
                     /translation="MTDCRYLIKRVIKIIIAVLQLILLFL"
     gene            complement(25930..26190)
                     /gene="yfhL"
                     /locus_tag="OO357_002419"
     CDS             complement(25930..26190)
                     /gene="yfhL"
                     /locus_tag="OO357_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster ferredoxin YfhL"
                     /protein_id="HCR5887335.1"
                     /translation="MSLLITKRCINCDMCEPECPNEAISMGDHIYEINSDKCTECVGH
                     YETPTCQKVCPIPNTIVKDPAHVETEEQLWDKFVLMHHADKI"
     gene            26405..>26784
                     /locus_tag="OO357_002420"
     CDS             26405..>26784
                     /locus_tag="OO357_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR5887336.1"
                     /translation="MPRTVTHNPDSPNNDDVLAASEKWDACKPPYTSAHMKICVAAAK
                     IILAASGVARCSKYEKENYLRIDFSKAGKVTFYAEFPKKMGLKGKKLGEWPELVIQLA
                     REKALGMADGGLRAESVHAALEMYR"
BASE COUNT         6609 a         6659 c         7022 g         6494 t
ORIGIN      
        1 ttggcgcaga aaaaaatgcc tgatgcgacg ctgcgcgtct tatactccca catatgacag
       61 attcagcaac ggatgcggtt tccccaactt gcctacttcc atacgtgtcc tccttaccag
      121 aaatttatcc ttaagctcct caataaccat tttcctgcta actaaattca tggtttaggt
      181 tgcataatga tacgcaacaa atgtataaca ttttctttac caaaagaaat aaacaaaagc
      241 gaccaacaaa agcatcggat tacggcagga gacataatgg catggctgaa agtactgtaa
      301 cggcagacag caaactgaca agtagtgata ctcgtcgccg catttgggcg attgtggggg
      361 cctcttcagg taatctggtc gagtggttcg atttctatgt ctactcgttc tgttcactct
      421 acttcgccca cattttcttc ccttccggga acacgacgac tcaactacta caaacagcag
      481 gtgtttttgc tgcgggattc ctgatgcgcc caataggcgg ttggctattt ggccgcatag
      541 ccgataaaca tggtcgcaaa aaatcgatgc tgttatcggt gtgtatgatg tgtttcggat
      601 cgctggttat cgcctgcctc ccaggttatg aaactatagg tacgtgggct ccggcattat
      661 tgcttctcgc tcgtttattt cagggattat ccgttggcgg agaatatggc accagcgcca
      721 cctatatgag tgaagttgcc gttgaagggc gcaaaggttt ttacgcatca tttcagtatg
      781 tgacgttgat cggcggacaa ctgctagccc tactggttgt cgtggtttta caacacacca
      841 tggaagacgc tgcactcaga gagtggggat ggcgtattcc tttcgcgtta ggagctgtgt
      901 tagctgttgt ggcgttgtgg ttacgtcgtc aattagatga aacttcgcaa caagaaacgc
      961 gcgctttaaa agaagctgga tctctgaaag gattatggcg caatcgccgt gcattcatca
     1021 tggttctcgg ttttaccgct gcgggctccc tttgtttcta taccttcacc acttatatgc
     1081 agaagtatct ggtaaatact gcgggaatgc atgccaacgt ggcgagtggc attatgactg
     1141 ccgcattgtt tgtattcatg cttattcaac cactcattgg cgcgctgtcg gataagattg
     1201 gtcgccgtac ctcaatgtta tgtttcggtt cgctggcagc catttttacc gttcctattc
     1261 tctcagcatt gcaaaacgtt tcctcgcctt atgccgcttt tggtctggtg atgtgtgccc
     1321 tgctgatagt gagtttttat acatcaatca gtggaatact gaaggctgag atgttcccag
     1381 cacaggttcg cgcattaggc gttggtctgt catatgcggt cgctaatgct atatttggtg
     1441 gttcggcgga gtacgtagcg ttgtcgctga aatcaatagg aatggaaaca gccttcttct
     1501 ggtatgtgac cttgatggcc gtggtggcgt ttctggtttc tttgatgcta catcgcaaag
     1561 ggaaggggat gcgtctttag tgacgggtca gttgccagac ggtatagccg gtgcttgcac
     1621 cggcgacatc ccaggccaaa tccttccagc tccagccgct cccttcgggg cggctatccc
     1681 aaagctcttt tgacgccccc aaactgacag agaacatcaa tccaaacatg gcactgcgat
     1741 cccggctcat cccctgatgc tgtgaatatt catttccggc ggcggaaagt atcgccgagg
     1801 cgataaagtg ttgagcttta tcctgcccac tccagctatc gttatccata tggctgcatt
     1861 cagaaagtaa caatagcgaa cagacaaaaa gaatacgcaa aacgtggcct ccaaaaaaca
     1921 aaccccgtac aggacggggt tgtgattaca ggatgcggct aattaatcgg tcgatgcgga
     1981 tacggcgcaa acggcggatg agtttacgaa ccttcaccgg ataatcggca atactttgca
     2041 gatcgcgata gtgcttaacg atggtggtac gctcgcggat cagctccagt tctttctctc
     2101 gctgcggcgc aagctccagt tgtggatcgt ggatcaaaat ggcgttttcc agatccagac
     2161 gccaggcgcg cgggttcagg ttattaccgg tgatcaacat ccacttatca tcaacccaca
     2221 tccctttcag gtgataggtg ttgtcgtcat ctttccataa ccgaaccact agctggtcag
     2281 tattgacgta atactgcaaa cggctcagga aacgacgcag attgatctca tagagataag
     2341 gcaatgcgcc aattatcttg aaaggttcat cttccggaat gtagaagtcg ttcgcggttt
     2401 tatcaccaac aataatttcg acctttttcc cttcgcgcag caactggata atattgcgca
     2461 caaggattgc tggcaggttg aagtatggcg tacagatggt tagtttctgc tcggcacaag
     2521 gcataagatg gaaaatggtc ttgttcaaca gactcgattt ccccagcccc actagcggcg
     2581 ttacggaaag ctgatcgttg tcggcatcgc cctggaaatg ataagcggca tcacgcagct
     2641 cctggcggaa cagacgaata tcgttcttga tttccgggct ttttggccga ttaacatcat
     2701 ccagacgatt aacgccgcgg ccattcataa tattctgtgt aacccattca aacataatgt
     2761 ctgacatctt acggttacgg atcagatgat aacggtcgta gcgatattta tcgtgttgat
     2821 gcaggtaaac atcgttcagg ctggcaccgc tataaagtac gctatcgtcg atgataaagc
     2881 ctttaaagtg cagaacacca agagcttcac gggtattgat tggaacgcca taaaccggaa
     2941 catctacgcc cggattttcc tgcgccatgc ggcagtacca gtcagcgtta gtgttagatg
     3001 ccgcagcgcc aatgcgtcca cgttgtgcac gatgccagtc gaccagcacc cgcacatcca
     3061 gttccggacg ctgcctttta gcctcataca acgcgttcag aatgcctttg ccaccgtcat
     3121 cctgttcgag atacagggcg acaatgcaaa tgcgctgctt cgcgctggct attttttcca
     3181 gcagcgtctc ccggaagtcg gcgggagcgt aaaagaaatc gacatcatca actgattgag
     3241 aaatcttggg tagttgggca aggtgttgtt gatgtttatt acgcttaaat tttgacaaca
     3301 tcacagtgca tttcttctct gttcattgaa gggtcctctg tgcaatgcag acgacataag
     3361 cgggcaataa taacaccagt cccgattaag tggtcaacat ttccagtacc ttactcatga
     3421 ttcctcgcgc cgggcaagat ttagcgtaag cccaacgatc ccctcttcga gctggatatc
     3481 aacgttaaac ccgagcttgc gggctagcgc caccatgcca cgattgtttg gcatcgtaat
     3541 accattcaga cgttgtagtc cgtgatttcg cgtataggta atcaactttt ccattaagcg
     3601 tcgacctaag cctaaccctt tgagatccga gcgaaccagt acagcaaatt cggcatcgat
     3661 gttatcagga tcggaaatcg cacgcgtgac gccgaggatc tcttccgttt gatcaatacg
     3721 tcgtaccgct acaaacgcca tttcccgatc gtagtcgatc tgtgtcatgt tggctaaatc
     3781 ttcatgggta aattcgttga tctcgctaaa gtagcggtaa taaagatctt ctttggtgac
     3841 tcgcgaaatg aattgctgaa gttgtggctc atcttctggc aaaatcgggc ggaacaagca
     3901 gcgttcaccg tttttcaatt ctacccattc ttccagctga tgcggataag ggcgcactgc
     3961 cagccgactc tcgttatcgc cttcaaacgg cgagatatcc agcgtgacat ccagcgcggt
     4021 aaattcactg ccagaagcca gcaaaggatg aatatccaga cgctgaattt ccgggcaatc
     4081 gacaatcaag ttggaaacct gcaccagaag ctggctcaag cctgcaacat ccaatgggcg
     4141 tagcgcactg cgcgcacgaa tctttttact tttgatcccc tgaataacca gatagcgggc
     4201 caggttcatg ttcagcggcg gcagtgcgac gacggcttga tcttcaggac gccactccac
     4261 accgccttca cccagcatga tcaacggccc gaaaaccgga tcgtgctcaa ccacaacccg
     4321 caactcctga gcgccagcac ggttagccat actttgcacc aacaggccgt ggacccgcgc
     4381 ctgtggccag gccattttta cgcgatcgaa aatagcgttc gccgcttgct ggacttcatt
     4441 ggctgtacgc aggtaaagca tgacgccctg aacttccgat ttatgtggaa tatccggcga
     4501 acgcaatttc agcgccaccg gataaccaat ctgttcggca atatgcaccg cttcggtgct
     4561 atcgctggca atccaggttg ggagcgtgtt catgccatac gcttgcagga tgggctgaac
     4621 ttcatgggta tcgagcgacg tagccccttc ggcaatcgcc tgttgcaaca gaagatgcgc
     4681 ttctgcggta ttggaagtca gattgctggg caacgccggc gtttcgcgta gttgcttctg
     4741 attacgccgg tactccacca tatgcataaa agcagtgatg gttccttccg gggtacggta
     4801 ggtcggcagc ccggcttcgc tgaataaacg tcgtgcctct tgcgaggagt gctcgccgca
     4861 ccagttcgtc agcaaagaga catatttgct gcggggatga tgctttaccg cttcaattaa
     4921 tacttgcgcg ctttctgttg cgggagcagc ggcgctgggc gaatgaataa ccatcagtgc
     4981 atcaaaatcc tggctgtgga gcagaatgtc cagcgttttg acataatgct cgctgctggc
     5041 gtcatcgcgc agatcgagcg ggttagagac tgccacatgt tctggcaatg catcgcgcaa
     5101 tttctggcag gtttcttcgc ttagcgttgc cagcttgcca ttgcgtgacc ataaggcatc
     5161 cagcgccagc gcggcaggcg cagcaccgtt gctgataatc atcaaccggt cgccacgcag
     5221 cgggcgcata tggctaaggg tttccaccgc cgaaaacagt tcgtgggtat cctgtacccg
     5281 caacaaaccg gcacgctgaa tagccgcatc ccatgccggg tccattcctg ccgtggtgtt
     5341 gagcagtcgc tgtgccgccg ggctacgtcc gcttttaatc accagaatcg gtttattacg
     5401 cgaggcacta cgggccgccg aaacaaagcg tcgcgcgtcg cttaactgtt cgagatagag
     5461 caggatggcg ctggttttac tgtcgcgcgc cagatagtcg agcaattcat caacgtcgat
     5521 atctaggctg tcgccgagcg caataaagta ggaaaagccc atctcacgct gttgcgccca
     5581 gtcgaggatg gtgttggaga cggcagccga ttgcgaaata aacgccagct tgccgcgttt
     5641 aatcggcaca ggcgaaaagc tggcattcag accttgccag ggagccagta aacccagact
     5701 gtttggtcca agcaggcgca tgttatggcg cagggcgcag gcgcggagat cttcgtgttg
     5761 cgatgccggg gcggaaagaa taatgcaggt tttacagcct ttctcgccga gctcttccag
     5821 aagagcaaga ttacggctgg cattggtaca taaaaccgca aggtcgggtg taaagggcaa
     5881 gctggcaata tccggccagg ccaacacacc caacaccgct ttccaggctg gcgtcaccgg
     5941 gagtaccggt ccgttaaagc ctcccgccag caggttacgc atcatcaggt aacctgcgcg
     6001 attgggtttc atcgacgcgc caattaccgc tatcgatttt ggtcgcagta gtgcttccag
     6061 tcctcgctga ctcataccgg tctcccgcag aagtgaccga atgattttaa acgctttcta
     6121 actgttctgc tgtgatgcta cccagatgtt gcgtttttcc tgccagatag cgtgttttaa
     6181 agcgggtaaa atgctcgcct aaccctgctg ccgccccggt atcgccggcc atatctaaca
     6241 gtgcgatggc tacctcggca gtacaatatt ggcctgcagc ctgggcttca cgcaggcgat
     6301 aggcagaaag ccgggaaaga tcgacggaaa tgacgggaag attatccaga tacggacttt
     6361 tacgaaacat cttgcgagct tccggccagg taccatcgag catgataaac agcggtggct
     6421 taccggcagg tggtgtgaag atcacttccc gttgctcatc agcatacgag gcgggaaaga
     6481 ccaccattgg ctgataatcc gggttttgta ccagatccag caaatcctgc gagggttcgg
     6541 tacgcgacca ttgaaacgca acggtatcag gcaaaatatc agcaatcaga cgcccggtat
     6601 tactgggctt cattggctcg gtgtcgaaca tcagcaaaca gaagcgactt tttgcttgtg
     6661 ctggggtaat tgtcgaacag agacataatt tctctggcaa aagacagcgt tggcagcgac
     6721 gaacgcgatt accgcgggca agaaaaggac gtgttgcgcg cgcaatacgc tcggcgcgta
     6781 actggagaac agcgttttcg gtcataagag agcgtcgaaa aaacgccatt gtcgcagagg
     6841 agaaaacggg gcacaagatg cgccccggta agattaaaga gattcgttca gccagctatc
     6901 gaacggcgct ttcggtactg cgccattaag catgtcgaca acctgaccgt ttttgaaaat
     6961 catgatcgtc ggtatactac gaattccaaa gcgactgctc aattcacgtt cagcttcggt
     7021 attcactttc acaaagcgca ctttaccgct acgctcttgc gcgacatctt caaaaattgg
     7081 tgcgaaatta cggcaggggc cgcaccacgg tgcccagaag tcgatcacca caggtagatc
     7141 atccttcagc aatttgtcga gtgtttcacc ggtcgcatta atcacctctc cgtcaaacaa
     7201 gtcgtgaccg cagcgtccgc attttgccgc atcttcgatc cgatcgtcgg gaatgcgatt
     7261 gatggcctga caatgggtac aaacggtatt cataactaac ctcgggatga gtaaggagac
     7321 ttatcggcaa cgacgccggt atgtttctaa tatgttacat attatcgggg acattgttcc
     7381 agacaacaat ctgttttatt caataggtat gatagtcgca ggctttgcta cgaaatcatg
     7441 gcgaaccgcg cggacttcgg gtaatctgcg cgcttcgcgc agcgctggtg gagaaaagca
     7501 tgaacgatga aatgaaaggt aaaagcggca aggtcaaagt gatgtatgtc cgcagtgatg
     7561 atgattctga taaacgtacc cacaacccgc gtaccgggaa agggggcggg cgtccaggaa
     7621 aatctcgtgc tgatggtggc cgtcgccccg cccgcgatga caaacagagt cagccccgtg
     7681 accgcaagtg ggaagattcg ccgtggcgca cggtttcccg cgcgccgggt gatgagacgc
     7741 cggaaaaggc cgatcacggt ggcatcagtg gtaaaagttt tattgatccg gaagtgttgc
     7801 gtcgtcagcg tgcggaagaa acccgcgtct acggcgaaaa cgcctgtcag gcactgttcc
     7861 agagccgtcc ggaagcgatt gttcgcgcct ggtttatcca gagtgtaacg ccgcgtttta
     7921 aagaagcctt gcgctggatg gcagcaaacc gcaaagcgta ccatgtggtg gatgaagcgg
     7981 aattgacaaa agcgtcaggt acggaacatc acggcggcgt ttgcttcctg atcaaaaagc
     8041 gtaacggtac aaccgtgcag cagtgggtaa tccaggcagg cgcgcaggat tgcgttctgg
     8101 cactggaaaa cgaatctaac ccgcataacc tgggtggcat gatgcgcagc tgcgcgcact
     8161 tcggcgtgaa aggtgtcgtg gtgcaggatg cggcactgct ggagtcgggg gcggctatcc
     8221 gtaccgcaga aggcggcgca gagcacgttc agccgattac tggcgacaac attgttaacg
     8281 tgctggatga tttccgtcag gcaggttaca ccgtagtgac cacttccagc gagcagggta
     8341 aaccgctgtt caaaaccagt ctgccagcga aaatggtact ggtgctgggt caggaatatg
     8401 aagggttacc ggatgccgca cgcgatccga acgatctgcg cgtgaagatt gacggtacgg
     8461 gcaacgttgc cgggctgaat atctctgtcg caaccggcgt tttgcttggt gaatggtggc
     8521 gtcagaataa agcctgataa atcagccggg tggcaactct gccatccggc atttccccgc
     8581 aaatttactc acactctgcc ggtaatactg gcatccagtc aatcggcgtc tcgccacgtt
     8641 gttccagcca ctgatttgcc agcacaaaat ggttgcagcc aaagaatcca cgatgcgccg
     8701 aaagcggcga cggatgcggt gctttcagta catgatggcg ttgcttatct ataatcgccc
     8761 ctttcttttg cgcatgtgat ccccacaaca aaaacaccac gccttcgcgg tgctggttaa
     8821 tcaggctgat gactttatca gtaaacgtct cccaaccgag gctggcgtgg gaatgcgcct
     8881 gacctgcgcg aaccgtcaac acagtattga gcagcagaac gccctgacgc gcccagcttt
     8941 cgagatagcc atgattaggg cgggtgaagc ccggaatagt attttccagc tctttataca
     9001 tattcaataa tgacggagga atggcaatgc cgggacgaac ggaaaatgcc agaccatgcg
     9061 cctgtcccgg tccgtgataa ggatcctggc cgagaatcac cactttaacg tcacccaact
     9121 cggtaaagcg gaacgcgtta aagacatctt tttgtggtgg atagatagtg acgccggact
     9181 gccgctcgct ggcgacggtc tgaagggtat taagaaaata aggttgttgc ttctcttcag
     9241 ccagcacgtc atgccaggtt aattcgttag ccatcttgct ctcctgcgaa tcttcaatcc
     9301 gcctagctta actgcttctt tctacagaac aaaatcatgc tcatttttta gacttaaacg
     9361 agtgcaaaaa aatgtaaatt ggtaaaattt cacacccgca taagttgatg taaaacaata
     9421 aaatccattt acgaccacaa tcgatgtgtg gtttttgttg atttaaatca aagaatcaag
     9481 ggtggttgag gagtatatat acactcaagc aacaatggtt ttaccaattg gccgcgacag
     9541 gctgaacaaa tcaaataatt ttgccgggga ggcatcacat gattacaggt atccagatta
     9601 ctaaagccgc taacgccgat ctgctgaact ctttctggct gctggacagc gaaaaaggcg
     9661 aagcacgttg catcgttgca aaagcaggtt atgcagaaga tgaagtggtt gcagtaagca
     9721 aactgggtga cattgaatac cgtgaagttc cagtagaagt gaaaccagaa gttcgcgttg
     9781 aaggtggtca acacctgaac gttaacgttc tgcgtcgtga aactctggaa gatgcagtta
     9841 agcatccgga aaaatatccg cagctgacca tccgtgtatc cggttatgca gttcgcttta
     9901 actctctgac tccggaacag cagcgcgacg ttatcgctcg tacctttact gaaagcctgt
     9961 aaggcttaag tagcaagtta ctaagcggaa gagggcgacc tcttccgctt tttttcgtta
    10021 atagaaaatg cgtaccgcgc aatagaccag caacagggca agcacgatat ttaactggcg
    10081 accatactgg cgaaacaatc gctgaaacag atgccccgcc agcgcccagc acacattgcc
    10141 aaacgtccca atcatcgcca gcaaaacgct gacgccaact acccagctta gcgcctgtgt
    10201 ttgcggcaga acaaacgtcg acagtgccgt aacaccgtac aaaatgattt tgacgttcac
    10261 aaactgcaaa gcaaagctgg cccaaaagct gattggtttt gcctgaagtc cgtcttcctt
    10321 tgttgggctg gtggcgattt tccacgccag ccagacaata tatgccgccc ccgcccaact
    10381 caaaaggtgt accgctgccg ggtcaatcac tgccagtgaa aatgaaatgc ccgcacacag
    10441 taacatcaca atcaaaaatc ccagactcat ccctgccagc acgcgggtac tttgacgaaa
    10501 tccatgcgac gtagcagagc taagggcgag aatattgttc ggtcctggcg tcatagcggt
    10561 aatcagggtg taagtccaaa aagcacttaa aaggatcggt gtcacttgtt atctcctctg
    10621 ttttggtaga cagtacccga cacggaacat ttaaaaaaat gaatgttttt atattctaaa
    10681 ttcgatattt tcgataggtt tggggttatg gatctgcgcc gttttattac gcttaaaacc
    10741 gtggtggaag agggttcctt tttgcgagct tcgcaaaaat tgtgctgtac acaatcgacg
    10801 gtgacttttc atattcagca gcttgagcag gagttttcag tccagttatt tgagaaaatt
    10861 ggccgacgga tgtgccttac ccgcgaagga aaaaagttgc tgccgcacat ttatgaactt
    10921 acccgagtga tggatacgct tcgtgaggct gccaaaaagg agtcagatcc ggacggggaa
    10981 ctacgcgttg tttcgggcga aacgttgctc tcttaccgaa tgccgcaggt gttgcagcgt
    11041 tttcggcaac gcgcaccaaa agtgcgttta tcgttgcagg cgctgaactg ttacgtgatc
    11101 cgtgatgcgc tactgaatga tgaggccgat gtcggcgtct tttatcgcgt agggaatgat
    11161 gatgccctga accgacgaga gttgggtgaa caatcgctgg tgctggtggc ttcaccacaa
    11221 attgcagatg tcgattttac tgagccggga agacataacg cctgtagctt tattatcaac
    11281 gaaccgcaat gtgtcttccg gcagatattt gagagcacgc tgcgacagcg gcggatcacg
    11341 gtggaaaaca ccattgagct tataagtatc gaaagcatca agcgttgtgt cgcggcgaat
    11401 atcggtgtca gttacttgcc ccactttgcg gtggaaaaag agattgagag cggagaatta
    11461 attgaattgc cttttggcga acagtcgcag accattacgg caatgtgcgc acattatgct
    11521 ggaaaagcgg ttagcccggc gatgcacatt tttattcagt gtgttgaaga gtgttttgtg
    11581 gttgcgtaaa aaaacaatgc cgggcttaaa cccggcattg agattactct tctgtcgttt
    11641 gcggtggcac gcccgttccg ctcggtttac ggcgcttacc aatatttttg gtgtcgcgat
    11701 ggcgtttttt cacccgcggc ttctctttct ctttagcttt tttcttctcg gcgcgttttg
    11761 ccagtacttt cttcgatggc ttgccggtct gcttctcgct tggcgcacgc gttttcgggc
    11821 gtaactcatc aataacgcga gctttaatcg gctcttcaat gtaacggcca accttgccca
    11881 gcagcaaatg gtcatgggct tccaccagcg aaattgcggt gcctttacga ccggcgcgcg
    11941 cggtacgtcc gatacgatgc aaataagtat cgccactgcg cggcatatcg aagttaaaga
    12001 cgtggctgac gtcaggaatg tcgataccgc gcgcggcaac atcggtggca accagtacgt
    12061 ttacgcggcc ttcggtcagg cgtttaattg cttcgttacg cttgccttgt accatctcac
    12121 cttcgagata gcagttgttg atgcccgctt cgcgcagcca gtttgccagc tcatgcacac
    12181 gttcacgctt acgcacaaac acgattgagc gagtcgcttc aggctgtttt aacaggtgca
    12241 ccagcaatgc ggttttatgt tccaggtcat cggcgcggta ataccactga tgaatttttt
    12301 tgcgctcacg ggtggacgga ttggcagaaa cttccaccgg atcttccagc agacgctcgg
    12361 caaagtcctg aatcgcatcg ccttccagcg ttgccgaaaa gagcagggtc tgtttacgcc
    12421 agcgagtttc gccagcaata tgttcgatat cctgagcgaa gcccatatcc agcatacggt
    12481 ctgcttcgtc gaggatcagc gtttcaaccg cgcggcaatc gaagttctct tcttttatgt
    12541 attgcagcag acgtccggtc gtggcgacca cgatgtcctg attttcgctg aacacttccg
    12601 cgtggttcat ataggctacg ccgccggtga tggtggcgat atccagatgc gtatgtttcg
    12661 ccagttcgcg ggcatgatcg gccacctgca tcgccagctc gcgagttggg gtgaggatca
    12721 aaatacgcgg cggaccggat ttcttacgtg ggaaatcgag caggtgctgc aacgctggca
    12781 gcagatacgc cgccgtttta ccggtgcctg tcggcgcaga accgagtaca tcacggccat
    12841 cgagcgcagg cggaatggcg gcagcctgaa tggcggtcgg gcgagtgaaa cctttatcct
    12901 ggagggcttc cagcaggctt tcgtcgagtt caagttcgga aaaagtcgtt acagtcatgt
    12961 tctacctctg tgtggggcgc tgattataga cgttacggct gcaatcttca tctgtttgta
    13021 tggatatcgc ttctcgggta ttgttctcac ccggtgctat cgccgttttt cctgcaaggt
    13081 tttattttca tgtcacagtc tacatccgtg cttcgtcgta atggatttac ttttaaacag
    13141 ttttttgttg ctcacgatcg ctgtgcgatg aaagtgggaa cggatggcat tttgctgggc
    13201 gcatgggcac cggtggctgg ggtgaaacgt tgccttgata tcggcgcggg tagcgggttg
    13261 ctggcactga tgctggcgca gcgaaccagt gacagcgtga taattgatgc cgttgagctg
    13321 gaaagtgaag ctgccgcgca ggcgcaggaa aatatcaacc agtccccgtg ggcagagcgg
    13381 attaacgtcc atacggcgga tattcagcag tggatcacac agcagacagt acgcttcgat
    13441 ttaatcatca gtaacccacc ttactatcag cagggagtag agtgcgcgac acctcaacgg
    13501 gaacaggctc gctataccac tacgcttgat cacccatcgc tgctgacctg tgcggcggag
    13561 tgcattaccg aagagggatt tttctgcgtg gtgctgccag agcagattgg taatggtttt
    13621 actgaactgg cattaagcat gggctggcat ttacgtttgc ggacggatgt ggcggaaaac
    13681 gaagcgcgac tgccgcatcg ggtgctgctg gcattctccc cgcaggcggg agaatgcttt
    13741 agcgatcgct tagtcattcg tgggccagac cagaactatt ccgaagcgta tacggcgctg
    13801 acccaggctt tttatctgtt tatgtaatga ttgccggggg aaaggatcga tggaccggaa
    13861 tgcgtgagca gttccgggta atccagcgta aaatgcagcc cccggctttc tttgcgcatc
    13921 atcgcgcagc gaacaatcaa ctcggcaacc tgtaccagat tacgcagctc cagcaaatta
    13981 tttgagacgc ggaaatgggc gtaatattcg tctatttctt gttggagcat ggttatccgc
    14041 cgcagggcgc gttccaggcg cttcgttgtg cgcacaatgc caacgtaatc ccacataaac
    14101 agccgtagct cgtgccagtt atgctgaatt actacccgtt cgtcagggtt ctcaacgcgg
    14161 ctttcatccc acggcggtaa cgtactgacg ccgtgggcat aaggcatacg tctggtgata
    14221 tcttctgccg ccgaccagcc atagaccaga cactccagca atgaattcga ggccatgcgg
    14281 ttagcgccgt gtaagccggt ataactcacc tcgccaatgg catacaagcc ctcgacgtcc
    14341 gtacgcccat gatcatcaac cattacacca cctcaggtat agtgtgcagc aggcacaatc
    14401 ggtaccggtt cttgtgtgag atcaatcccc agcccgagca atttttcata aatcatcggg
    14461 aaatgctggc gaataaaatc ggcgggctta tggctgatgt cgaggaacat acaatctgcg
    14521 ccgaggcgtt tcatttcatg gtcaatggcg cgggcgacaa tatcgcgcgg ggccagttcg
    14581 ccgcgctcat caaaatcggg cataaaacgc gtgccatccg ggcgcttgag ataagcgcct
    14641 tcgccgcgca gtgcttccgt taacaggaaa ttgcgtgcct gtgggtgata cagtgcggta
    14701 gggtggaact gattaaattc gagattggca acccgacagc ccgcgcgcca cgccatagca
    14761 atgccatcgc cagaagaaat atccggattg gtggtgtact gatacacctt cgacgcaccg
    14821 ccggttgcca gtaccactgc ttttgcgtgg caggtttcca ccgtttcttt atttcggttc
    14881 catacccacg cgcctacaac ccgtcgcgtg cccggcaggc caattttgtc agagacaatc
    14941 agatcaactg cgttgctgcg ctccagcaca cgaatattcg gatggttctg cgccttgctc
    15001 accagcgtgg attgtacttc tctaccggtg gcgtcggcgg catgaagaat acgacggtga
    15061 ctatgtccac cttcacgggt cagatggtag ctttcttcgc cattcggttg aacgtgggta
    15121 tcaaacaaaa ccccctggtc aattaaccat ggcacacagg aacgagcatt gctggcgaca
    15181 aattcaactg catggtgatc gcaaatacca gccccggcaa tcaatgtgtc ttccacatgc
    15241 gagtcaatgc tgtcagtttc atcaaacacg gcggcaatac cgccctgggc ataaaatgtt
    15301 gaaccttccg ttaccgggcc tttacttaga acgatgacct gatgctggtc agccaggcgt
    15361 agcgccagtg aaagtccggc tgcgccgcta ccaataatca acacgtcaca tgaatgttca
    15421 gggagagtat tcattttctt tgtttaattt actaaacatt gtttggtcgg catagcatca
    15481 tgttgtgcga ataaacacct gctattttaa tatttgttac agtggctaaa cacgttgacg
    15541 cagggcggcg agaaaaagag aagttactgg ctggtggagg attaggtggt gaaataaaaa
    15601 ggccgttggg ttactcttca ggcagttaaa tgggcatttc tacacagata atgcgatgtt
    15661 cagattctgt agacttataa tgatagataa tgatccgtct acagcatgac aaacaaaaac
    15721 agatgcgtta cggaacttta caaaaacgag acactctaac cctttgcttg ctcaaattgc
    15781 agctaatgga gtggcgtttc gatagcgcgt ggaaatttgg tttggggaga ctttacctcg
    15841 gatgagcgag cagttaacgg accaggtcct ggttgaacgg gtccagaagg gagatcagaa
    15901 agcctttaac ttactggtag tgcgctatca gcataaagtg gcgagtctgg tttcccgcta
    15961 tgtgccgtcg ggtgatgttc ccgatgtggt acaagaagct tttattaaag cctatcgtgc
    16021 gctggattcg ttccggggag atagcgcttt ttatacatgg ctgtatcgga ttgctgtaaa
    16081 tacagcgaaa aattacctgg ttgctcaggg gcgtcgtcca ccttccagtg atgtggatgc
    16141 cattgaagct gaaaacttcg aaagtggcgg cgcgttgaaa gaaatttcga accctgagaa
    16201 cttaatgttg tcagaagaac tgagacagat agttttccga actattgagt ccctcccgga
    16261 agatttgcgc atggcaataa ccttgcggga gctggatggc ctgagctatg aagagatagc
    16321 cgctatcatg gattgtccgg taggtacggt gcgttcacgt atcttccgag cgagggaagc
    16381 tattgataac aaagttcaac cgcttatcag gcgttgacga tagcgggata ctggataagg
    16441 gtattaggca tgcagaaaga acaactttcc gctttaatgg atggcgaaac gctggatagt
    16501 gagctgctta acgaactggc tcataaccca gaaatgcaga aaacctggga aagctatcac
    16561 ttaatccgtg attcaatgcg gggtgatact cccgaggtgc tccatttcga tatctcttca
    16621 cgcgtgatgg ccgccattga agaagagcca gtacgtcaat cggcgacatt gatcccggaa
    16681 gcccagcctg cgccgcatca atggcagaaa atgccattct ggcagaaagt acgtccgtgg
    16741 gcggcacagc ttacccaaat gggcgtagcc gcatgcgtat cgcttgcagt tatcgttggc
    16801 gtccagcact ataatggaca atctgaaacg tcccagcagc ccgaaacgcc ggtatttaat
    16861 acactgccga tgatgggtaa agccagcccg gtaagcctgg gagtaccttc tgaagcgacc
    16921 gcaaacaatg gtcaacagca gcaggtacag gagcagcgtc gtcgcattaa tgcaatgttg
    16981 caggattacg aactgcaacg ccgactccac tctgaacagc ttcagtttga gcaggcgcaa
    17041 acccagcaag ccgctgtaca ggtgccagga attcaaactt taggaacgca atcgcagtaa
    17101 tgaagcaact ttggtttgcc atgtcattag tgacaggtag cctgttattc tctgctaacg
    17161 cctcggccac tcccgcgtcc ggggcgttat tacagcagat gaacctggcc agtcagtcac
    17221 tgaattacga gctgtcattc atcagcatca ataaacaggg tgttgagtct ctgcgttatc
    17281 gacatgcacg cctcgataac cgtcctcttg cacaattgtt gcaaatggat ggcccgcgcc
    17341 gggaagtggt acagcgcggc aatgaaatca gctattttga accgggactt gaaccgttca
    17401 cgcttaatgg cgattacatt gttgattctc tgccatcgct tatctatacc gatttcaaac
    17461 gcctttctcc ttactacgac tttatctccg tcgggcgcac gcgtattgct gatcgtcttt
    17521 gcgaagtcat tcgcgtggtt gcccgagatg gtacacgcta cagctacatc gtgtggatgg
    17581 acaccgaatc gaaattaccg atgcgggttg atctccttga tcgcgatggt gaaacgctgg
    17641 aacaatttcg cgtgattgct tttaacgtca atcaggatat cagcagcagt atgcagacgc
    17701 tggcgaaggc aaatttgccg ccgttgcttt ctgttcctgt aggtgaaaaa gctaaattca
    17761 gctggacgcc aacctggttg ccacagggtt ttagcgaagt ttccagtagt cgacgtccgc
    17821 taccgacgat ggacaacatg cctatcgaat cacgtctcta ttccgacgga ttattcagct
    17881 tctcggtaaa cgttaaccgc gctacgccat cgagcaccga tcagatgttg cgcaccggac
    17941 gcagaaccgt cagtacaagc gtacgtgata acgccgaaat caccattgtc ggtgaactgc
    18001 cgccgcaaac ggcgaaacgc attgccgaga atattaagtt cggggcagcg caatgatcaa
    18061 agagtgggct accgtcgtct cctggcaaaa cgggcaggcg ctggtcagtt gtgatgttaa
    18121 agcttcatgc agcagctgcg cttcacgcgc cggttgcggt agccgcgtgt tgaataaact
    18181 tggcccgcaa accacgcata ccattgtcgt accctgtgat gagccgttag tgccggggca
    18241 aaaagtggag ttggggatcg ccgaaggcag cctgcttagc tccgcattac tggtttatat
    18301 gtcgccgctg gtgggattat tcctcatcgc ttcgctattt cagctactct ttgcttctga
    18361 cgtcgcagca ttatgtggtg cggtcctcgg cggtattggc gggttcctga ttgcgcgcgg
    18421 ctactcgcgt aagtttgctg cccgggcaga atggcaaccg atcatcttaa gcgtggcatt
    18481 gccgcctggc cttgtgcgat ttgaaacatc ttcggaagac gcgagccagt gatttcactg
    18541 gctttgttgc attttgcata acaaatgcga ggacgtttcc agataccaca atccatacgg
    18601 tatgaacatt tcctcgcctc aatgttgtag tgtagaatgc ggcgtttcta ttaatacaga
    18661 cgttaagctc agaacagcga cttctaaaag cctggttaac cgggcattaa ggcacaataa
    18721 tcatacttta tgaagaatat acgtaacttt tcgatcatag ctcacattga ccacggtaaa
    18781 tcgacgctgt ctgaccgtat tatccagatc tgcggtggcc tgtctgaccg tgaaatggag
    18841 gcgcaggttc tcgattccat ggatcttgag cgtgagcgtg gcattaccat caaagcgcaa
    18901 agcgtgacgc tggactacaa agcgtctgac ggcgaaacct atcagcttaa ctttatcgac
    18961 accccgggcc acgtagactt ctcctatgaa gtttcccgtt cgctggctgc ctgtgaaggt
    19021 gcattgctgg tggtcgacgc cgggcagggc gtagaagcgc aaaccctggc aaactgctac
    19081 accgccatgg aaatggatct cgaagtggtg ccggtactga acaagattga cctgccggca
    19141 gccgatcctg aacgcgtggc ggaagaaatt gaagatatcg tcggcatcga cgccaccgat
    19201 gcagtgcgct gttcagcgaa aaccggcgtt ggcgtgcagg acgttctcga acgtctggtg
    19261 cgcgacattc cgccgccgga aggcgatccg gaaggcccgt tgcaggcact aattatcgac
    19321 tcctggttcg acaactacct gggcgttgtt tcacttatcc gtattaaaaa cggcaccctg
    19381 cgtaagggcg acaaagtgaa agtcatgagt accgggcaga cctataacgc cgaccgtctg
    19441 ggcatcttca cgccgaaaca ggttgaccgc actgaactga aatgtggcga agtaggctgg
    19501 ctcgtatgtg cgattaaaga tatccacggc gctccagtcg gcgatacctt aacgctggcg
    19561 cgtaatccgg cagaaaaggc gctgcctggc tttaagaaag tcaaaccgca ggtatacgcc
    19621 ggtctgttcc cggtaagttc cgacgactat gaagccttcc gtgacgcgct gggtaaactc
    19681 agcctgaacg atgcctcact gttctatgag ccggaaagct ccagcgcgct gggctttggt
    19741 ttccgctgcg gcttccttgg cctgctgcac atggagatca tccaggagcg tctggaacgt
    19801 gaatacgatc tggatctgat caccactgcg ccgaccgtag tgtatgaagt tgaaaccacg
    19861 tcaagggaag ttatctacgt cgacagccca tccaagctgc ctgcggtaaa taacatttac
    19921 gaactgcgcg agccgattgc agagtgtcac atgctgctgc cgcaggcata tctcggcaac
    19981 gttattacgc tgtgcgtaga aaaacgcggc gtgcagacca atatggttta ccacggtaat
    20041 caggtggcgc tgacgtacga gatcccgatg gcggaagtgg tgctcgactt cttcgatcgc
    20101 ctgaaatcta cctcgcgtgg ttatgcgtct ctggattaca acttcaaacg cttccaggcg
    20161 tccgacatgg tacgtgtaga cgtattaatc aacggtgaac gtgttgatgc gctggcgttg
    20221 atcacccacc gtgataattc gcaaaaccgc ggtcgcgagt tggtggagaa gatgaaagat
    20281 ctgatcccac gccagcagtt tgatatcgcc attcaggcag cgattggtac gcacattatt
    20341 gcgcgatcca ccgtgaaaca gctgcgtaaa aacgtactgg ctaaatgtta tggcggcgat
    20401 atcagccgta agaaaaagct gctgcagaag cagaaagaag gtaagaaacg catgaagcag
    20461 atcggtaacg ttgagctgcc gcaggaagcg ttcctcgcca ttctgcacgt cggcaaagac
    20521 aacaaataac ccttaggagt tggcatggcg aatatgtttg ccctgattct ggtgattgcc
    20581 acactggtga cgggcatttt atggtgcgtg gataaattct ttttcgcacc taaacggcgg
    20641 gaacgtcagg cagcggcgca ggcggctgcc ggtgactcac tggataaagc aacgttgaaa
    20701 aaggttgcac cgaagcctgg ctggctggaa accggagctt ctgtttttcc ggtgctggct
    20761 atcgtattga ttgtacgttc gtttatttat gaaccgttcc agatcccgtc aggttcgatg
    20821 atgccgactc tgttaatcgg tgattttatt ctggtagaga agtttgctta tggcattaaa
    20881 gatcctatct accagaaaac gctgatcgaa accggtcatc cgaaacgcgg cgatatcgtg
    20941 gtctttaaat atccggaaga tccaaagctt gattacatca agcgcgcggt gggtttaccg
    21001 ggcgataaag tcacttacga tccggtctca aaagagctga cgattcaacc gggatgcagt
    21061 tccggccagg cgtgtgaaaa cgcgctgccg gtcacctact caaacgtgga accgagcgat
    21121 ttcgttcaga ccttctcacg ccgtaatggt ggggaagcga ccagcggatt ctttgaagtg
    21181 ccgaaaaacg aaaccaaaga aaatggaatt cgtctttccg agcgtaaaga gacactgggt
    21241 gatgtgacgc accgaattct gacagtgccg attgcgcagg accaggtggg gatgtattac
    21301 cagcagccag ggcaacaact ggcaacctgg attgttccgc cgggacaata cttcatgatg
    21361 ggcgacaacc gcgacaacag cgcggacagc cgttactggg gctttgtgcc tgaagcgaat
    21421 ctggtcggtc gggccacggc tatctggatg agcttcgata agcaagaagg cgaatggccg
    21481 actggtgtgc gcttaagtcg cattggcggc atccattaat agccatcttc gttcacgttg
    21541 tcgccgttat ggcgacaacg tgaattattt atgagataaa tctcccgtgg ctaacgacat
    21601 cccccgtcgt tgtgtataga atattccccc gaagtttaag gttggcacct tcaggttgcc
    21661 acggcacacg aaacagcgtt ggtccccata taccgctaaa ctgaaactgc agcgaagcag
    21721 ttagcagagc catgtatatc aggtctgttt cgtgtgctga attgttgacg catttattta
    21781 ttggtatcgc atgaacccca tcgtaattaa tcggcttcaa cggaagctgg gctacacttt
    21841 taatcatcag gaactgttgc agcaggcatt aactcatcgt agtgccagca gcaaacataa
    21901 cgagcgttta gaatttttag gcgactctat tctgagctac gttatcgcca atgcgcttta
    21961 tcaccgtttc cctcgtgtgg atgaaggcga tatgagccgg atgcgcgcca cgctggtccg
    22021 tggcaatacg ctggcggaac tggcgcgcga atttgagtta ggcgagtgct tacgtttagg
    22081 gccaggtgaa cttaaaagcg gtggatttcg tcgtgagtca attctcgccg acaccgtcga
    22141 agcattaatt ggtggcgtat tcctcgacag tgatattcaa accgtcgaga aattaatcct
    22201 taactggtat caaactcgtc tggacgaaat tagcccaggc gataaacaaa aagatccgaa
    22261 aacgcgcttg caagaatatt tgcagggtcg ccatctgccg ctgccgactt atctggtagt
    22321 ccaggtacgt ggcgaagcgc acgatcagga atttactatc cactgccagg tcagcggcct
    22381 gagtgaaccg gtggttggca caggttcaag ccgtcgtaag gctgagcagg ctgccgccga
    22441 acaggcgttg aaaaaactgg agctggaatg agcatcgata aaagttactg cggatttatt
    22501 gccatcgtcg gacgtccgaa cgttggcaaa tccacattgt tgaacaaact gctggggcag
    22561 aaaatctcca tcacctcccg caaggcgcag acaactcgtc accgcattgt ggggatccat
    22621 actgaaggcg cgtatcaggc gatctacgtc gatacaccgg gcctgcatat ggaagaaaaa
    22681 cgcgccatta accgcctgat gaacaaagcg gcgagcagct ctattggcga tgttgagctg
    22741 gtgatttttg tcgttgaagg cacccgctgg acgccggacg acgaaatggt gctcaacaaa
    22801 ctgcgcgaag gcaaagcgcc ggtaatcctc gcggtgaaca aagtggacaa cgtgcaggag
    22861 aaagccgatc tgctgccgca cctgcagttc ctggcaagcc agatgaactt cctcgatatc
    22921 gtgccaatct ctgccgaaac cgggctgaat gttgacacta ttgcggcaat cgtgcgtaag
    22981 catctgcctg aagcgactca tcacttcccg gaagattaca tcaccgatcg ctcacagcgt
    23041 tttatggcgt ctgaaatcat ccgcgaaaaa ctgatgcgtt tccttggcgc tgaactgccg
    23101 tactccgtga ccgtggagat cgaacgtttc gtctctaacg aacgcggcgg ttgtgacatc
    23161 aacggtttga ttctcgttga gcgtgaaggg cagaagaaga tggttattgg caacaaaggg
    23221 gccaagatca aaaccatcgg gattgaagcg cgtaaagaca tgcaggaaat gttcgaagcg
    23281 cctgtccacc ttgaactgtg ggtaaaagtg aaatcgggtt gggccgacga cgaacgcgca
    23341 ctgcgcagtc tcggttatgt tgacgatctt taagagtcac tccgatggaa ggctggcagc
    23401 gcgcatttgt cctgcatagt cgcccgtgga gcgaaaccag cctgatgctg gacgtcttca
    23461 cagaggaatc gggtcgcgtg cgtctggttg ccaaaggcgc acgctctaaa cgctctactc
    23521 tgaaaggtgc attacagcct ttcacccctc tcttgctacg ttttggcggg cgtggcgaag
    23581 tcaaaacgct gcgcagtgct gaagctgtct cactggcgct gccattaagc ggtatcacgc
    23641 tttacagcgg tctgtacatc aacgaacttc tctcccgcgt actggaatac gagacgcgct
    23701 tctctgaact ctttttcgat tacttgcact gcattcagtc tcttgcaggt gacactggta
    23761 cgccagaacc cgcgctgcgt cgctttgaac tggcactact cgggcatctg ggctatggcg
    23821 tcaattttac ccattgcgcg ggtagcggcg agccggtaga tggcaccatg acgtatcgtt
    23881 atcgcgaaga aaaagggttt atcgcaagcg tcgttatcga caataaaacg ttcaccggaa
    23941 ggcagttaaa agcgttaaac gcgcgggaat ttcctgacgc agacacacta cgcgccgcga
    24001 aacgctttac ccgaatggcg cttaagccgt atcttggcgg taaaccatta aagagcaggg
    24061 aactgttccg gcagtttatg cctaagcgaa cggtgaaaac acattatgaa tgatgaggat
    24121 tgtcatggct gaattactgt taggcgtcaa cattgaccat attgcaacgc tgcgtaacgc
    24181 acgcggtact gcttacccgg atccggtgca ggcggcgttt attgccgagc aggcgggagc
    24241 ggacggcatt accgtgcatt tacgtgaaga tcgccgccac attactgacc gcgatgtgcg
    24301 catcctgcgg cagacgctgg atacccgaat gaatctggag atggcagtga ccgaagagat
    24361 gctggcgatc gccgttgaga cgaagccaca tttttgctgc ttggtaccgg aaaagcgtca
    24421 ggaagtaaca accgaaggcg gcctggacgt tgcagggcag cgtgaaaaaa tacgcgatgc
    24481 ctgcaaacgt ctggcagatg ccgggattca ggtttctctg tttatcgacg ccgatgaaga
    24541 gcagatcaaa gctgcggcag aggttggcgc accgtttatc gagatccaca ccggttgcta
    24601 tgctgatgcc aaaactgacg ccgaacaggc gcaagagctg gtgcgtatcg ccaaagccgc
    24661 gacctttgcc gctagtctcg gtctgaaagt taacgccgga cacggtctga cctatcacaa
    24721 cgtgaaagcc attgccgcca tccctgagat gcatgaactg aatatcggtc atgccattat
    24781 tggccgtgca gtgatgaccg gactgaaaga tgcggtggca gaaatgaagc gtctgatgct
    24841 ggaagcgcgt ggctaatggc aatattaggt ttaggcacgg atatcgtaga gatcgctcgc
    24901 atagaagcag tgatcgcccg atccggtgag cgcctggcac gccgcgtatt aagcgataac
    24961 gaatgggcaa tctggaaaac gcaccaccag ccggtgcgtt ttctggcgaa gcgttttgct
    25021 gtgaaagaag ccgcagcaaa agcgtttggc accgggatcc gcaatggtct ggcgtttaat
    25081 caatttgaag tattcaatga tgagctcggc aaaccacggc tacggctatg gggtgaggca
    25141 ttaaaactgg cggaaaagct cggcgttgta aatatgcatg taacgctggc agatgaacgc
    25201 cactatgctt gtgccacggt aattattgaa agttaaaata ataccgttct ctttaaaaag
    25261 taaaaccccc gaactggtaa ggaacggggg ttttactttt taactttaat tttgtgcagc
    25321 atgcaccctc aacaacagcg attatggatc agtgtactta tcttttacaa gcaacttgca
    25381 atctttagca taaaaactcg agcctttacg aagaaagcaa tattgatgga aagattaacg
    25441 tgaccgccaa ttcgtaagta cattaaaatt ggcttcgtta ttgaagattt tgctgtgctt
    25501 tacaccatgc cacagaattc ccccattgaa acgagtggtg tcgtcaaagc tctggtgtgg
    25561 agtgcagcat gcaccctcaa taactcgcac gttcagtttt ggggagatgt aagggctaat
    25621 ctgaatggct gcattccttg tttaaggaaa aacgaatgac tgattgccga tacctgatta
    25681 aacgggtcat caaaatcatc attgctgttt tacagctgat ccttctgttc ttataacaca
    25741 aggaaacgta cttaaggtgc gtccggtgaa ccaatcggac gcgcctttaa taactataaa
    25801 taagtgtctt ggcagatact atataaatta acttagtgaa tgattatgct aatgtcatca
    25861 attaaataaa tataatggcg ttaaggcttc ccagtaatat aattaatact ctacttccag
    25921 agtagaatat taaattttat ccgcgtggtg catcagcaca aatttatccc acaactgttc
    25981 ttctgtctcg acatgcgccg gatctttcac aatagtattg gggatcgggc acaccttctg
    26041 gcaggttggt gtctcgtagt gccctacgca ttcggtacac ttatcgctgt taatttcgta
    26101 gatatgatct cccattgaaa tcgcctcatt cgggcattcg ggttcacaca tatcacaatt
    26161 aatacagcgt ttagtaatta gtaaagacat ttcaatggat taccgttaaa tcattttaaa
    26221 atcagtaagt tgtatcgagt ttgtatgctt tactgtcatt aacttactgt atgttgatcc
    26281 agtgtattta accttgataa actcagtcca gcaacacaaa accgcaacac attgcatttt
    26341 gtcccgtaga aaagacttgt atgtgtgagc ttgttttctg cgcctacgca gataaggatt
    26401 gagaatgccg cgcactgtaa cacataatcc ggatagcccc aataatgacg atgttttagc
    26461 cgcatctgaa aaatgggacg cctgtaaacc cccctatacc agcgcacaca tgaaaatctg
    26521 tgttgctgcc gccaaaatca tcctcgctgc ttccggcgtg gctcgctgtt ccaaatacga
    26581 aaaagagaac tatctccgta tcgatttcag caaagccggt aaggttacat tttacgccga
    26641 gtttccaaaa aagatgggcc tcaaaggtaa aaagctcggc gagtggccgg agctcgttat
    26701 ccagctggcg cgcgaaaaag cactaggtat ggctgacggt ggactgcggg cagagtccgt
    26761 acatgcagcg ctggaaatgt accg
//
LOCUS       DAJTRC010000055        26310 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.055, whole genome shotgun sequence.
ACCESSION   DAJTRC010000055 DAJTRC010000000
VERSION     DAJTRC010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 26310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 26310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..26310
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.055"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            346..639
                     /locus_tag="OO357_002421"
     CDS             346..639
                     /locus_tag="OO357_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309701.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgL domain-containing protein"
                     /protein_id="HCR5887337.1"
                     /translation="MFCAIYRSSKRDQTYLYVEKKDDFSRVPEELMKGFGQPQLAMIL
                     PLDGRKKLVNADIEKVKLALTEQGYYLQLPPPPEDLLKQHLSVMGQKTDDTNK"
     gene            711..1370
                     /locus_tag="OO357_002422"
     CDS             711..1370
                     /locus_tag="OO357_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HCR5887338.1"
                     /translation="MYQHHNWQGALLDYPVSKVVCVGSNYAKHIKEMGSAVPEEPVLF
                     IKPETALCDLRQPLAIPSDFGSVHHEVELAVLIGATLRQATEEHVRKAIAGYGVALDL
                     TLRDVQGKMKKAGQPWEKAKAFDNSCPLSGFIPAAEFTGDPQNTTLGLSVNGEKRQQG
                     TTADMIHKIVPLIAYMSKFFTLKAGDVVLTGTPDGVGPLQSGDELTVTFDGHSLTTRV
                     L"
     gene            1462..1908
                     /locus_tag="OO357_002423"
     CDS             1462..1908
                     /locus_tag="OO357_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309703.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HCR5887339.1"
                     /translation="MSDVPFWQSKTLDEMSDAEWESLCDGCGQCCLHKLMDEDTDEIY
                     FTNVACRQLNIKTCQCRNYERRFEFEPDCIKLTRENLPTFEWLPMTCAYRLLAEGKDL
                     PAWHPLLTGSKAAMHGERISVRHIAVKESEVIDWQDHILNKPDWAQ"
     gene            complement(2115..3015)
                     /gene="hlyE"
                     /locus_tag="OO357_002424"
                     /pseudo
     CDS             complement(2115..3015)
                     /gene="hlyE"
                     /locus_tag="OO357_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415700.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin HlyE"
     gene            3388..3807
                     /locus_tag="OO357_002425"
     CDS             3388..3807
                     /locus_tag="OO357_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translesion error-prone DNA polymerase V
                     autoproteolytic subunit"
                     /protein_id="HCR5887340.1"
                     /translation="MLFIKPADLREIVTFPLFSDLVQCGFPSPAADYVEQRIDLNQLL
                     IQHPSATYFVKASGDSMIDGGISDGDLLIVDSAITASHGDIVIAAVDGEFTVKKLQLR
                     PTVQLIPMNSAYSPITISSEDTLDVFGVVIHVVKAMR"
     gene            3807..5075
                     /gene="umuC"
                     /locus_tag="OO357_002426"
     CDS             3807..5075
                     /gene="umuC"
                     /locus_tag="OO357_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase V catalytic protein"
                     /protein_id="HCR5887341.1"
                     /translation="MFALCDVNAFYASCETVFRPDLWGKPVVVLSNNDGCVIARNAEA
                     KALGVKMGDPWFKQKDLFRRCGVVCFSSNYELYADMSNRVMSTLEELSPRVEIYSIDE
                     AFCDLTGVRNCRDLTDFGREIRATVLQRTHLTVGVGIAQTKTLAKLANHAAKKWQRQT
                     GGVVDLSNLERQRKLMSALPVDDVWGIGRRISKKLDAMGIKTVLDLADTDIRFIRKHF
                     NVMLERTVRELRGEPCLQLEEFAPTKQEIICSRSFGERITDYTSMRQAICSYAARAAE
                     KLRSEHQYCRFISTFIKTSPFALNEPYYGNSASVKLLTPTQDSRDIINAATRSLDAIW
                     QAGHRYQKAGVMLGDFFSQGVAQLNLFDDNAPRPGSEQLMAVMDTLNAKEGRGTLYFA
                     GQGIQQQWQMKRAMLSPRYTTRSSDLLRVK"
     gene            complement(5121..5651)
                     /gene="dsbB"
                     /locus_tag="OO357_002427"
     CDS             complement(5121..5651)
                     /gene="dsbB"
                     /locus_tag="OO357_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707082.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein DsbB"
                     /protein_id="HCR5887342.1"
                     /translation="MLRFLNQCSQGRGAWLLMAFTALALELTALWFQHVMLLKPCVLC
                     IYERCALFGVLGAALIGAIAPKTPLRYVAMVIWLYSAFRGVQLTYEHTMLQLYPSPFA
                     TCDFMVRFPEWLPLDKWVPQVFVASGDCAERQWDFLGMEMPQWLLGIFIAYLIVAVLV
                     VISQPFKAKKRDLFGR"
     gene            complement(5797..7338)
                     /gene="nhaB"
                     /locus_tag="OO357_002428"
     CDS             complement(5797..7338)
                     /gene="nhaB"
                     /locus_tag="OO357_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707083.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na(+)/H(+) antiporter NhaB"
                     /protein_id="HCR5887343.1"
                     /translation="MEISWGRALWRNFLGQSPDWYKLALIIFLIVNPLIFLISPFVAG
                     WLLVAEFIFTLAMALKCYPLLPGGLLAIEAVFIGMTSAEHVREEVAANLEVLLLLMFM
                     VAGIYFMKQLLLFIFTRLLLSIRSKMLLSLSFCVAAAFLSAFLDALTVVAVVISVAVG
                     FYGIYHRVASSRTEDTDLQDDSHIDKHYNVVLEQFRGFLRSLMMHAGVGTALGGVMTM
                     VGEPQNLIIAKAAGWHFGDFFLRMSPVTVPVLICGLLTCLLVEKLRWFGYGETLPEKV
                     REVLQQFDDQSRHQRTRQDKIRLIVQAIIGVWLVTALALHLAEVGLIGLSVIILATSL
                     TGVTDEHAIGKAFTESLPFTALLTVFFSVVAVIIDQQLFSPIIQFVLQASEHAQLSLF
                     YIFNGLLSSISDNVFVGTIYINEAKAAMESGAITLKQYELLAVAINTGTNLPSVATPN
                     GQAAFLFLLTSALAPLIRLSYGRMVWMALPYTLVLTLVGLLCVEFTLAPVTEWFMQMG
                     WIATL"
     gene            7559..8278
                     /gene="fadR"
                     /locus_tag="OO357_002429"
     CDS             7559..8278
                     /gene="fadR"
                     /locus_tag="OO357_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HCR5887344.1"
                     /translation="MVIKAQSPAGFAEEYIIESIWNNRFPPGTILPAERELSELIGVT
                     RTTLREVLQRLARDGWLTIQHGKPTKVNNFWETSGLNILETLARLDHESVPQLIDNLL
                     SVRTNISTIFIRTAFRQHPDKAQEVLATANEVADHADAFAELDYNIFRGLAFASGNPI
                     YGLILNGMKGLYTRIGRHYFANPEARSLALGFYHKLSALCSEGAHDQVYETVRRYGHE
                     SGEIWHRMQKNLPGDLAIQGR"
     gene            complement(8330..9862)
                     /locus_tag="OO357_002430"
     CDS             complement(8330..9862)
                     /locus_tag="OO357_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoVR family protein"
                     /protein_id="HCR5887345.1"
                     /translation="MATIDSMNKDTTRLSDGPDWTFDLLDVYLAEIDRVAKLYRLDTY
                     PHQIEVITSEQMMDAYSSVGMPINYPHWSFGKKFIETERLYKHGQQGLAYEIVINSNP
                     CIAYLMEENTITMQALVMAHACYGHNSFFKNNCLFRSWTDASSIVDYLIFARKYITEC
                     EERYGVDEVERLLDSCHALMNYGVDRYKRPQKISLQEEKARQKSREEYLQSQVNMLWR
                     TLPKREEEKTVAEARRYPSEPQENLLYFMEKNAPLLESWQREILRIVRKVSQYFYPQK
                     QTQVMNEGCATFWHYTILNHLYDEGKVTERFMLEFLHSHTNVVFQPPYNSPWYSGINP
                     YALGFAMFQDIKRICQSPTEEDKYWFPDIAGSDWLETLHFAMRDFKDESFISQFLSPK
                     VMRDFRFFTVLDDDRHNYLEISAIHNEEGYREIRNRLSSQYNLSNLEPNIQIWNVDLR
                     GDRSLTLRYIPHNRAPLDRGRKEVLKHVHRLWGFDVMLEQQNEDGSIELLERCPPRMG
                     NL"
     gene            10192..11490
                     /gene="dadA"
                     /locus_tag="OO357_002431"
     CDS             10192..11490
                     /gene="dadA"
                     /locus_tag="OO357_002431"
                     /EC_number="1.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid dehydrogenase"
                     /protein_id="HCR5887346.1"
                     /translation="MRVVILGSGVVGVASAWYLNQAGHEVTVIDREPGAALETSAANA
                     GQISPGYAAPWAAPGVPLKAIKWMFQRHAPLAVRLDGTQFQLKWMWQMLRNCDTSHYM
                     ENKGRMVRLAEYSRDCLKALRAETNIQYEGRQGGTLQLFRTEQQYENATRDIAVLEDA
                     GVPYQLLESSRLAEVDPALAEVAHKLTGGLQLPNDETGDCQLFTQNLARMAEQAGVKF
                     RFNTPVDQLLCDGEQIYGVKFGDEVIKADAYVMAFGSYSTAMLKGIVDIPVYPLKGYS
                     LTIPIAQEDGAPVSTILDETYKIAITRFDNRIRVGGMAEIVGFNTELLQPRRETLEMV
                     VRDLYPRGGHVEQATFWTGLRPMTPDGTPVVGSTRFKNLWLNTGHGTLGWTMACGSGQ
                     LLSDLLSGRTPAIPYEDLSVARYSRGFTPSRPGHLHGAHS"
     gene            11500..12570
                     /gene="dadX"
                     /locus_tag="OO357_002432"
     CDS             11500..12570
                     /gene="dadX"
                     /locus_tag="OO357_002432"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolic alanine racemase DadX"
                     /protein_id="HCR5887347.1"
                     /translation="MTRPIQASLDLQALKQNLSIVRQAAPHARVWSVVKANAYGHGIE
                     RIWSALGATDGFALLNLEEAITLRERGWKGPILMLEGFFHAQDLEIYDQHRLTTCVHS
                     NWQLKALQNARLKAPLDIYLKVNSGMNRLGFQSDRVLTVWQQLRAMANVGEMTLMSHF
                     AEAEHPDGISGAMARIEQAAEGLECRRSLSNSAATLWHPEAHFDWVRPGIILYGASPS
                     GQWRDIANTGLRPVMTLSSEIIGVQTLKAGERVGYGGRYTARDEQRIGIVAAGYADGY
                     PRHAPTGAPVLVDGVRTMTVGTVSMDMLAVDLTPCPQAGIGTPVELWGKEIKIDDVAA
                     AAGTVGYELMCALALRVPVVTV"
     gene            complement(12738..14152)
                     /locus_tag="OO357_002433"
                     /pseudo
     CDS             complement(12738..14152)
                     /locus_tag="OO357_002433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016243131.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(14763..16499)
                     /locus_tag="OO357_002434"
     CDS             complement(14763..16499)
                     /locus_tag="OO357_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HCR5887348.1"
                     /translation="MDATTIISLFILGSILVTSSILLSSFSSRLGIPILVIFLAIGML
                     AGVDGVGGIPFDNYPFAYMVSNLALAIILLDGGMRTQASSFRVALGPALSLATLGVLI
                     TSGLTGMMAAWLFNLDLIEGLLIGAIVGSTDAAAVFSLLGGKGLNERVGSTLEIESGS
                     NDPMAVFLTITLIAMIQQHESSVSWMFVVDILQQFGLGIVIGLGGGYLLLQMINRIAL
                     PAGLYPLLALSGGILIFALTTALEGSGILAVYLCGFLLGNRPIRNRYGILQNFDGLAW
                     LAQIAMFLVLGLLVNPSDLLPIAIPALILSAWMIFFARPLSVFAGLLPFRGFNLRERV
                     FISWVGLRGAVPIILAVFPMMAGLENARLFFNVAFFVVLVSLLLQGTSLSWAAKKAKV
                     VVPPVGRPVSRVGLDIHPENPWEQFVYQLSADKWCVGAALRDLHMPKETRIAALFRDN
                     QLLHPTGSTRLREGDVLCVIGRERDLPALGKLFSQSPPVALDQRFFGDFILEASAKYA
                     DVALIYGLEDGREYRDKQQTLGEIVQQLLGAAPVVGDQVEFAGMIWTVAEKEDNEVLK
                     IGVRVAEEEAES"
     gene            complement(16594..17508)
                     /gene="ldcA"
                     /locus_tag="OO357_002435"
     CDS             complement(16594..17508)
                     /gene="ldcA"
                     /locus_tag="OO357_002435"
                     /EC_number="3.4.17.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muramoyltetrapeptide carboxypeptidase"
                     /protein_id="HCR5887349.1"
                     /translation="MSLFHLIAPSGYSIKQHAALRGIQRLTDAGHQVNNVEVIARRCE
                     RFAGTETERLEDLNSLARLTTPNTIVLAVRGGYGASRLLADIDWQALVARQQHDPLLI
                     CGHSDFTAIQCGLLAQGNVITFSGPMLVANFGADELNAFTEHHFWLALRNETFTIEWQ
                     GEGPTCQTEGTLWGGNLAMLISLIGTPWMPKIENGILVLEDINVHPFRVERMLLQLYH
                     AGILPRQKAIILGSFSGSTPNDYDAGYNLESVYAFLRSRLSIPLITGLDFGHEQRTVT
                     LPLGAHAILNNTREGAQLTISGHPVLKM"
     gene            17608..18219
                     /gene="emtA"
                     /locus_tag="OO357_002436"
     CDS             17608..18219
                     /gene="emtA"
                     /locus_tag="OO357_002436"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     EmtA"
                     /protein_id="HCR5887350.1"
                     /translation="MKLRWFAFLIVLLAGCSSKHDYTNPPWNAKVPVQRAMQWMPISQ
                     KAGAAWGVDPQLITAIIAIESGGNPNAVSKSNAIGLMQLKASTSGRDVYRRMGWSGEP
                     TTSELKNPERNISMGAAYLNILETGPLAGIEDPKVLQYALVVSYANGAGALLRTFSSD
                     RKKAISKINDLDADEFLDHVARNHPAPQAPRYIYKLEQALDAM"
     gene            complement(18221..18955)
                     /locus_tag="OO357_002437"
     CDS             complement(18221..18955)
                     /locus_tag="OO357_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar brake protein"
                     /protein_id="HCR5887351.1"
                     /translation="MSHYHEQFLKQNPLAVLGVLRDLHKAAIPLRLSWNGGQLISKIL
                     AITPDKLVLDFGSQAEDNIAVLKAQHITITAETQGAKVEFTVEQLQQSEYLQLPAFIT
                     VPPPTLWFVQRRRYFRISAPLHPPYFCQTKLADNSTLRFRLYDLSLGGMGALLETAKP
                     AELQEGMRFAQIEVNMGQWGVFHFDAQLISTSERKVIDGKNETITTPRLSFRFLNVSP
                     TVERQLQRIIFSLEREAREKADKVRD"
     gene            19156..19410
                     /locus_tag="OO357_002438"
     CDS             19156..19410
                     /locus_tag="OO357_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HCR5887352.1"
                     /translation="MGIIAWIIFGLIAGIIGKLIMPGRDGGGFFLTCILGIVGAVVGG
                     WLATMFGIDGSISGFNLHSFLVAVVGAILVLGVFRLLRRE"
     gene            complement(19460..20920)
                     /locus_tag="OO357_002439"
     CDS             complement(19460..20920)
                     /locus_tag="OO357_002439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor"
                     /protein_id="HCR5887353.1"
                     /translation="MHASFAASSDLGYLQVSGSQLKQDFLGLPHGVNNDIAGKHGKMI
                     NSSADDKRGIVKLGFTPRENDEYTLTYIKQDGEKDNPPYSGNCGQKSRYWQWPEYDKE
                     SFYYQGATQLNDRFTLKSRLYRDTFENTLMMYNSLADLKNKKGSYSHYSDYSDGAGLQ
                     LAADVRENDLLSFAVNWKDDVHREKGAPHAAYDRYEDRTWSLASEYQWAAADNVDVVA
                     GISYDWRDSVEAKKHEKDGSITHYDDNNQSAFNWQVMGKYHFANEDTLALSYYDRTRF
                     PTLKERYTTSKPAYNQIAIVNPQLKPERARGVDLTWNGAFTRDWGFEVSVYYNRVSDA
                     ILSHNINADTIQNQNSGTVDYSGLDAGIKGKISNILDVGLSYALIHADAKRKDIGKIT
                     DLPTQTMTAWMTLKPWEPLSVTLSEEARSSSYSNSDGSQKAAGFAVTHIRADYTLGHG
                     FSVNASVNNLFDTQYAYSEGFIEEGRNFWAGVEYTF"
     gene            complement(20857..21414)
                     /locus_tag="OO357_002440"
     CDS             complement(20857..21414)
                     /locus_tag="OO357_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Plug domain-containing protein"
                     /protein_id="HCR5887354.1"
                     /translation="MRLKKRYLCTALSLAFTQQAVAAQESDTLTVWSSPVSSTTTTVL
                     DQPTMKALDKQNVAQALSVVPGVVLQKSGSRNEEQVKVRGFDSRQVPVYFDGVPIYVP
                     YDGNLDLARILTNNLGAVEVSKGYSSLLHGPNQMGGAINITTLKKQVWDIARDGAVVR
                     TMPTICMLHLPPAAILGICKSAVAS"
     gene            complement(21440..22294)
                     /locus_tag="OO357_002441"
     CDS             complement(21440..22294)
                     /locus_tag="OO357_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707097.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ModD protein"
                     /protein_id="HCR5887355.1"
                     /translation="MIFLSQAQIDALLLEDIQGGDLTTRALNIGHQPGYIAFFHRQGG
                     CVSGVSVACKMLTTLGLTIDDAVSDGSQANAGQRLIRAQGNAAALHQGWKAVQNVLEW
                     SCGVSDYLAQMLALLRERYPDGNIACTRKAIPGTRLLASQAILAAGGLIHRAGCAETI
                     LLFANHRHFLHDNQGWSGAIAQLRRHAPEKKIVVEADTPKEAIAALRAQPDVLQLDKF
                     SPQQATEIAQIAPSLAPHCTLALTGGINLTTLKNYLDCGIRLFITSAPYYAAPADIKV
                     SLQPTASI"
     gene            complement(22294..23103)
                     /locus_tag="OO357_002442"
     CDS             complement(22294..23103)
                     /locus_tag="OO357_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HCR5887356.1"
                     /translation="MLIDDIDFADLYLQQLKLAHRTEKTPDHWDQRAEKMAENCASPT
                     DSYLQQLIAKIDLQGAQTLFDMGCGPGTVSLALADKLTTVYGVDYSQGMLNVAARRAA
                     ALKADNVHWIQRAWEEDWSDLPRCDIAVASRSTLVADMRHAMSKLNNQARLRVYTTHL
                     VSTSFVSPAIQRAAGREVIELPNYIFALNVLYQMGIYAHVDFIRGQNCQQDNSTWERF
                     EQNVSWSLGALNDDERERLYHWYQQQDARALAPASRDWALIWWDSVPQEAL"
     gene            complement(23113..23871)
                     /locus_tag="OO357_002443"
     CDS             complement(23113..23871)
                     /locus_tag="OO357_002443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5887357.1"
                     /translation="MTLLAVRHASLGYSRHPVLRDVSFTLSQGTICCLLGANGCGKTT
                     LMRSILGVIPLLKGEILLDSLSVQTLSHRQRAQAIAWVPQAHDGIFAFSVLDMVLMGL
                     APTIGAFSVPGKQERLKATEQLEKLGILHLATRRWNTLSGGERQLALIARALVQQPRL
                     LLLDEPASSLDFGHQIQLLDTLAQLKNNGMTMLMSTHHTLHANAIADSIIQVEPDGRV
                     TQGLPTEQLTTNKLAALYRVSADQIHHHLSAISR"
     gene            complement(23868..24848)
                     /locus_tag="OO357_002444"
     CDS             complement(23868..24848)
                     /locus_tag="OO357_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HCR5887358.1"
                     /translation="MRIVNGCILLLAAISITFAAVSGAYHLDMQQLLALILRQENESV
                     QEQIVFWQIRVPRILAALLLGAALAGAGTTYQGMLRNPLVSPDILGVSAGAGLGACAA
                     ILWGLPIVYIQLYAFCGGLMVVAGVWLITRRVTRHDPILTLVLVGIALGTLCGAGISL
                     IKTLADPYTQLPSITFWLLGGLSTVTLRDLCYAAPIILIGSLPLFFLRWRMNLLTLSD
                     DEARSLGLNVTRLRFGLIVCATLITASTVAIAGIIGWIGLVVPHIARLLTGHNHQQLL
                     PMAMCTGAILLLLTDTLARSIGTTEIPLGILTAFVGAPFFLLLLLRGGRQ"
     gene            complement(24848..25870)
                     /locus_tag="OO357_002445"
     CDS             complement(24848..25870)
                     /locus_tag="OO357_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707101.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HCR5887359.1"
                     /translation="MPITRRTFAQALASTLLLQSLPSFSQTVNRFASQSLPEAQNITR
                     IVSAGAPADLLLLAVAPEKMVGFSSFDFARQALIPLPEHIRQLPRLGRLAGRASTLSL
                     EGLMALHPDLVVDCGNTDETLISQARQVSEQTQIPWLLLNGKLAQSAEQLTTLGKTLG
                     EEHRAAEQANLASHFVGEAQAFATSPAANLRFYAARGPRGLETGLQGSLHTEAAELLG
                     LHNVAQIADRHGLTQVSMENLLRWQPDIILVQEAVTADFIRRDPLWQGVKAVAEQRIL
                     FLSGLPFGWLDAPPGINRLLGLRRLHAWLDPAINRQFKSDMQHYAQLFWHCSLSDADY
                     QKLVAS"
BASE COUNT         6584 a         6439 c         6803 g         6484 t
ORIGIN      
        1 tggcctttgc tgaactcaac attgacattc ttacccgcag caaagctcgt ggaggttagc
       61 agtgcaagca gcaatacagc cttactgatg ctcttgattt tcattggtag tctcctgttc
      121 agaatgagta cgatgaactg ttataatata acaatccctt acgggagaat tccagtggct
      181 tttctgcgtt cggtaaaatc ccggcgcggc attttttgcc ggggggatta cttataaagg
      241 ggcatggact caacaaaccg cagaaatcta cagctggctg gcttgtggcg cgggtttcat
      301 tgatgttagc ttatgccaaa acctggcatc ttaaagagta aaagtatgtt ttgtgcgatt
      361 tatcgaagca gcaagcgtga ccagacctat ttatatgtcg aaaaaaaaga cgatttttcg
      421 cgtgttcctg aggaactgat gaaaggtttt ggtcagcctc agttagcgat gattctgccg
      481 ttggatgggc ggaagaaact ggtcaatgct gatattgaaa aggttaaact ggcattaacc
      541 gagcaaggtt actatttgca gttaccgcca ccacccgaag atttgctgaa gcaacatctt
      601 tccgtcatgg ggcagaaaac agacgacact aacaaataac cgatatccgg cggtggcatt
      661 atctttgtcg gcgcgggttt tcatatccac gataaggtga ggggaacgtt atgtatcaac
      721 atcacaactg gcaaggtgcg ctgctggatt atccggtgag taaagtagtc tgtgttggca
      781 gtaactatgc caaacatatt aaagagatgg gcagcgcagt gcccgaagag ccagtgctgt
      841 ttattaaacc agaaacggca ctgtgcgatc tgcggcagcc attggcgatc ccatccgatt
      901 tcggttcagt tcatcatgaa gtcgaactgg cggtgttgat tggcgcgacg ctgcgtcagg
      961 caacggaaga gcatgtccgc aaagccattg ccggttacgg cgtggcgctt gatctgacat
     1021 tgcgtgatgt tcagggaaaa atgaagaaag ccgggcagcc gtgggaaaag gctaaagcgt
     1081 ttgataactc ttgtccgctt tccgggttta tccccgcggc ggaatttacc ggcgatccac
     1141 aaaatacaac gctgggcctg agcgtaaacg gcgaaaaacg ccagcaaggt acgactgcgg
     1201 acatgatcca taaaatcgtt ccgttgatcg cttatatgag caagtttttt accctcaagg
     1261 ccggtgacgt tgtgttgaca ggcacgcctg atggcgtcgg cccgttgcaa agcggtgatg
     1321 agctgacagt cactttcgat gggcattctt tgacaactcg cgttttgtaa tactttttgc
     1381 cgccagaaag cggcggcaaa acttgcatcg ctgtgccaga ctggttataa ggtgcgtttt
     1441 aacgtaatgg cggaacacct gatgagcgat gtacctttct ggcaaagtaa aaccctggac
     1501 gaaatgagcg atgcggaatg ggagtcgttg tgtgatggtt gcggtcagtg ttgcttgcat
     1561 aaactgatgg atgaagacac cgacgaaatc tacttcacta acgtcgcctg tcgccagctc
     1621 aatattaaaa cctgtcaatg tcggaactac gaacgtcgtt tcgagtttga acccgactgc
     1681 attaaattaa cccgtgaaaa tctgccaaca ttcgaatggc tgccaatgac ctgcgcttat
     1741 cggttgctgg cggaaggtaa agatttacct gcgtggcatc cgctacttac tggttcgaaa
     1801 gcggcaatgc atggtgaacg tatctctgtg cgtcatatcg cagtgaaaga atcagaagtg
     1861 attgactggc aggatcatat cttaaataaa cctgactggg cgcagtgata aatttaatag
     1921 atgctgatgg gggctttctt aatatacgtc tggatgctcg ccatttcgta gccctgtttt
     1981 tgaaagcgct ctgtcataat gagagttacc cgatacctta ttatcaacag gtaactctca
     2041 atctttaaaa aacgtctgtg tttttaaatg tgataaacct tataccttga gtacatggag
     2101 agaataatcg cttatcagac ttcaggtact tcaaagagtg tctttttacc gtgtcttttc
     2161 tgatactcat tacaggtgtt aatcattttt ttggccgctg cttttagcaa agaaagcatt
     2221 aaatcatcat aatcaacata gaatctggtt gtttcagttt ccgtttttat ctccccgatg
     2281 gcggctattt cggtggttaa tttcaatttg gcggcatcga tatctttatt cgcttgttta
     2341 accgtgttag acagggtggt aaagaaactc tgcacagatt ttaacttgtt cttcaattct
     2401 ggaatcagtt tcccttcaac tacgcccgca gcaatagaat aggaaatgat taaaccaaat
     2461 ggaccggcga cgacatcggc tgcggcaccg gcatacgctt ccttcctgat tttatctacc
     2521 tgtgactgga aatagctgct tttttctgaa aaatcattgg ttaactggct atctaacgcc
     2581 agcagtttcc cggaagcgtt gttgaaactt tgtgagctta ccagcaggga tttttgcgct
     2641 tcattcagct tcgtgatgcc gtcatccagt accttaatga ggggcggatg ctttcttctc
     2701 attgtactca tcaaatagca aaatatacgc tgcgagcaat tgcgtcgcaa caccacacca
     2761 ttcatacact gtttgggttg cttcaaaata cttatcctgg ctatccataa gtaaggtttt
     2821 aatatcgccg actaaaacgg aggctgcctg tgaatactcc tgtttaaagc gacttaactc
     2881 ttttatggtt tcatcaaagg tctgccaggg gatgacctga tcgagatatt tattataaag
     2941 atctaatgct ccatctgcgg tttcgattgc gtttttaact acttctaccg ttttatctgc
     3001 aacgatttca gtcataatca ttcgcctctt taaatatata aattgtaatg aaactcctgt
     3061 tttacaacta ttaataaatt ttacttcatc taattcatag ttagccgggc gggatgcgtc
     3121 aatgtcttta tttctattaa tatgataaat atcaaacaat gtttaatgtc attatggcga
     3181 atgcttctat tctatttttt tgccgggtga tatttttcat ttctgctgga tgagcgtcgt
     3241 cgccagaagg ccacgtgagc acaggataag agaacgaaaa atcagcagtc tatgcagcga
     3301 caaatattga tagcctgaat cagtattgat ctgctggcaa gaacagacca ctgtatataa
     3361 aaacagtata acttcaggca gattattatg ttgtttatca agcctgcgga tctccgcgaa
     3421 attgtgactt ttccgctatt tagcgatctt gttcagtgtg gctttccttc accggcagca
     3481 gattacgttg aacagcgcat cgatctgaat caactgttga tccagcatcc cagcgcgact
     3541 tacttcgtca aagcaagtgg tgattctatg attgatggtg gaattagtga cggtgattta
     3601 ctgattgtcg atagtgctat taccgccagc catggtgata ttgtcatcgc tgctgttgac
     3661 ggcgagttta cggtgaaaaa attgcaacta cgcccgacgg tacagcttat tcccatgaac
     3721 agtgcgtact cgcccattac catcagtagc gaagatacgc tggatgtctt tggtgtggtg
     3781 atccacgtcg ttaaggcgat gcgctgatgt ttgccctctg tgatgtaaac gcgttttatg
     3841 ccagctgtga gacggtgttt cgccctgatt tatggggtaa accggtggtt gtgctatcga
     3901 ataatgacgg ttgcgttatc gcccgaaacg ctgaggcaaa ggcgctgggc gttaaaatgg
     3961 gcgatccctg gttcaaacaa aaagatctgt ttcgtcgctg tggtgtggtt tgctttagca
     4021 gcaattatga gctttacgca gacatgagca atcgggtgat gtcgacgctg gaagaactat
     4081 cgccccgcgt cgagatttac agtattgatg aagcattctg cgatctgaca ggtgtgcgta
     4141 attgtcgcga tctgactgat tttggcagag aaattcgcgc aacggtgcta caacgtaccc
     4201 atcttactgt tggtgtgggg atcgcccaga ccaaaacgtt ggctaagctt gccaatcatg
     4261 cggcaaaaaa atggcagcgg cagacgggtg gggtggtgga tttatcaaat ctggaacgcc
     4321 agcgtaaatt aatgtctgct ctccccgtgg atgacgtctg ggggattgga cggcggatca
     4381 gcaaaaaact ggacgcgatg gggatcaaaa ccgttctcga tttggcggat acagatatcc
     4441 ggtttatccg taaacatttt aatgtcatgc tcgaaagaac ggtgcgtgaa ctgcgcggcg
     4501 aaccctgttt gcaactggaa gagtttgcac cgacgaagca ggaaattatc tgttcccgct
     4561 cgtttggtga acgcatcacg gattatacgt cgatgcggca ggccatttgt agttacgctg
     4621 cccgggcggc ggaaaaactt cgcagtgagc atcaatattg tcggtttatc tccacattta
     4681 ttaagacgtc accatttgcg ctcaatgaac cttattacgg caatagcgcg tcggtaaaac
     4741 tgctgacgcc cactcaggac agcagggata tcattaacgc tgctacgcga tctctggatg
     4801 ccatctggca agcgggccat cgctaccaaa aagcgggcgt gatgctgggg gatttcttca
     4861 gtcagggagt cgcgcagctc aatttattcg atgacaacgc accgcgcccc gggagtgagc
     4921 aattgatggc ggtaatggat actctgaatg ctaaagaggg cagaggaaca ctctattttg
     4981 ccgggcaggg gatccaacaa caatggcaga tgaagcgagc tatgctttca ccacgttata
     5041 caacgcgaag ttctgattta ctgcgggtca aataaatata gcggcaggaa aaaagcgctc
     5101 ccgcaggagc gccgaatgga ttagcgaccg aacagatcac gttttttcgc tttaaacggc
     5161 tgggaaatca ccaccagcac cgcgacaatc aggtaagcga taaaaatacc gagcagccac
     5221 tgcggcattt ccatacctaa aaaatcccac tgacgctcgg cgcaatcgcc agaggcgaca
     5281 aacacttgcg gcacccattt atccagcggc agccattccg ggaaacgaac cataaaatca
     5341 caggtggcaa atggcgaagg atagagctga agcatggtgt gctcgtaagt taactgcaca
     5401 ccgcggaacg cactatacaa ccagataacc atcgctacat aacgcagcgg agttttcggg
     5461 gcgatcgcgc caatcagcgc agcacccaga acgccgaata acgcgcagcg ttcataaata
     5521 cagagcacgc aaggtttcag taacatcaca tgctggaacc acagcgccgt cagttccagt
     5581 gccagagcag taaacgccat caacagccac gcgccccggc cttgtgaaca ttggttcaaa
     5641 aatcgcaaca taatcatttc cctgcaatat gcatagagtg cgcagtttaa accaattcat
     5701 tcgctgcgcc accaggaggc agcaaaaata ggacgcaatt ggacaattat cgggcgaaaa
     5761 ggcaaaccgg gcatttatat gcccggtaag ttgttatcaa agcgttgcta tccagcccat
     5821 ttgcataaac cattcggtta caggggcaag cgtaaactcg acgcagagca agccgacgag
     5881 tgtcagtacg agggtgtaag gcagggccat ccacaccatg cggccataag agaggcgaat
     5941 caatggcgcg agtgcagagg tcagcaggaa caggaacgca gcctgaccgt tcggcgtagc
     6001 gacagagggc agattggtac cggtattaat ggcgaccgcc agcagctcgt attgcttcaa
     6061 cgtgatagcg ccactttcca ttgccgcttt cgcttcgttg atataaatcg tccccacgaa
     6121 gacgttatcc gaaatggatg acagcagacc gttgaaaata tagaacagcg acagctgagc
     6181 atgttcagat gcctgcaaca caaactgaat aatcggcgaa aacagttgtt ggtcgataat
     6241 caccgcgact accgagaaaa acaccgtcaa cagtgcggtg aatggcagag attcggtgaa
     6301 ggctttaccg atagcatgtt catcggtgac accggtcaat gatgttgcca gaataatgac
     6361 tgacaaacca atcaagccaa cttccgccag atgcaacgcc agcgcagtca ccagccagac
     6421 gccaataatc gcctggacaa tcagacggat tttatcctga cgggtacgct ggtggcggct
     6481 ttgatcgtca aactgttgca acacttcgcg gactttctcc ggcagcgttt caccgtagcc
     6541 aaaccaacgc agcttctcta ccagcaggca ggttaacagg ccacaaatca gaaccggaac
     6601 ggtcaccggc gacatgcgca ggaagaaatc gccaaaatgc cagccagccg ctttagcgat
     6661 gatcaggttc tgtggttcgc ccaccatggt cattacgccg cctaatgcgg tgccgacacc
     6721 ggcatgcatc atcaggctac gcagaaagcc acggaactgt tccagaacca cgttgtaatg
     6781 cttgtcgata tgactatcgt cttgcaggtc ggtgtcttcg gtacgggaag aggctacgcg
     6841 atgataaata ccataaaaac cgactgcaac gctgatcacc accgccacga cggttaaggc
     6901 atcgaggaac gcggagagga acgctgccgc cacgcaaaaa gagagcgaca gcagcatttt
     6961 ggagcgaatg cttaacagca aacgggtaaa tatgaacagc aacagctgtt tcataaaata
     7021 gatacccgcc accataaaca tcaataacag caagacttca agatttgccg ccacctcttc
     7081 acggacgtgt tccgcgctgg tcatgccgat gaataccgct tcgatagcca acagaccacc
     7141 ggggagtagc gggtagcatt tcagggccat cgccagagtg aaaataaatt ccgctaccag
     7201 caaccagccc gcgacgaaag ggctgatgag gaaaattaac gggtttacga ttaagaaaat
     7261 gatgagggcg agtttgtacc agtcggggga ctggcccaaa aaattgcgcc atagcgcgcg
     7321 gccccaggag atctccatga tggtttccct taccttacaa ataatcaatg atgtttttat
     7381 gtttaaacgc aaagcttaac ggtcaggcag gagtgaggca agtcttgata gtcaagggga
     7441 aagagatgcg gaaaatgaag ccttgatccc tttttcttct ttttgtctgc tatcagcgta
     7501 gttagccctc tggtatgatg agtccaactt tgttttgctg tgttatggaa atctcactat
     7561 ggtcattaag gcgcaaagcc cggcgggttt cgcggaagag tacattattg aaagtatctg
     7621 gaataaccgc ttccctcccg ggactatttt gcccgcagaa cgtgaacttt cagaattaat
     7681 tggcgtaacg cgtactacgt tacgtgaagt gttacagcgt ctggcacgag atggctggtt
     7741 gaccattcaa catggcaagc cgacgaaggt gaataatttc tgggaaactt ccggtttaaa
     7801 tatccttgaa acactggcgc gactggatca cgaaagtgtg ccgcagctta ttgataattt
     7861 gctgtcggtg cgtaccaata tttccactat ttttattcgc accgcgtttc gtcagcatcc
     7921 cgataaagcg caggaagtgc tggctaccgc taatgaagtg gccgatcacg ccgatgcctt
     7981 tgccgagctg gattacaaca tattccgcgg cctggcgttt gcttccggca acccgattta
     8041 cggtctgatc cttaacggga tgaaagggct gtatacgcgc attggtcgtc actatttcgc
     8101 caatccggaa gcgcgcagtc tggcgctggg cttctaccac aaactgtcgg cgttgtgcag
     8161 tgaaggcgcg cacgatcagg tgtacgaaac agtgcgtcgc tatgggcatg agagtggcga
     8221 gatttggcac cggatgcaga aaaatctgcc gggtgattta gccattcagg ggcgataatc
     8281 ccttccgttt aaagagcaac cccctcaaac gaggggtttt ttgttgtttt tacagatttc
     8341 ccattcttgg cgggcaacgt tccagcaact cgatgctgcc gtcttcgttt tgctgttcga
     8401 gcatcacatc aaatccccac aggcgatgca catgcttcag gacttctttg cgcccccgat
     8461 ccagcggtgc gcgattatgt ggaatataac gcagcgtcag cgaacggtcg ccgcgcaaat
     8521 ccacgttcca gatctgaata ttcggctcca gattacttaa gttatattgc gacgataacc
     8581 ggttacggat ctcccgataa ccttcttcat tatgaatagc ggaaatctcc agataattat
     8641 gccgatcatc atccagcacg gtgaagaagc ggaaatcacg catcactttc ggtgacagaa
     8701 actggctgat aaaactctca tctttgaaat cacgcatcgc gaaatgcagc gtttccagcc
     8761 agtcggaacc ggcgatatcc gggaaccagt atttgtcttc ttccgttggc gactgacaaa
     8821 tccgtttaat atcctggaac atggcgaacc cgagggcata cgggttgatg ccgctgtacc
     8881 acgggctgtt ataggggggc tggaagacca cattggtgtg gctgtgcaaa aactccagca
     8941 taaaacgttc cgttactttc ccttcatcat acagatggtt aaggatggta tagtgccaga
     9001 aggtcgcaca gccttcgttc atcacctgag tctgtttttg cggataaaaa tactggctca
     9061 ccttacgcac aatacgcagg atttcacgct gccatgattc cagcagtggc gcatttttct
     9121 ccataaaata gagcaggttt tcttgtggtt cggacggata gcggcgcgct tcagcaaccg
     9181 ttttctcttc ctcgcgcttc ggcagggtac gccagagcat attgacctga ctttgtagat
     9241 actcttcgcg acttttctgc cgggctttct cttcttgcag cgagattttt tgcgggcgtt
     9301 tgtaccggtc cacgccgtag ttcatcagcg cgtggcacga gtccagaagc cgttctactt
     9361 catcaacgcc ataacgttct tcgcactcgg taatatattt acgggcaaaa atcagataat
     9421 cgacaatcga actggcgtcg gtccaactgc ggaataagca attgttttta aagaaagagt
     9481 tatgtccata gcaggcgtgt gccatcacca gcgcctgcat agtaatggtg ttctcttcca
     9541 tcaagtaagc gatacacggg ttagagttaa tgacgatttc ataggccagc ccttgctgac
     9601 cgtgcttata cagccgttca gtctcgataa actttttacc gaatgaccag tgcggatagt
     9661 taattggcat gccgacgctg gaataggcat ccatcatctg ttctgaggtt atcacttcaa
     9721 tctggtgcgg gtaggtatcc agccggtaga gtttcgccac ccggtctatc tctgccagat
     9781 aaacatccag caggtcgaac gtccagtcgg gtccatcgct caaacgtgtg gtgtccttat
     9841 tcatagaatc gatcgtcgcc atacgcgcac ctcattgttg tcggcgctct ctgtgtggag
     9901 cacctcattt caagcataga acacctgtta aaaaccgcgt cgccggagaa tttttttctt
     9961 tgcgatttct tattatcaga gtgccactaa tccgcttctg aacggaattt tatgctggat
    10021 aaaaagggcg ttcagcagga gatactaaag acgccatatt gccgcagagt cagggagatg
    10081 tgagtcagct caccataaaa aagccgtatg ttgaataata ttttcaactg agttatcaag
    10141 atgtaattag attattattc ttttactgta tctaccgtta tcggagtggc tatgcgagtt
    10201 gtcatactgg gaagtggtgt ggtaggcgtt gccagcgcct ggtacttaaa tcaggcagga
    10261 catgaggtca ccgtcattga tcgggagccg ggggccgccc tggaaaccag tgccgcgaat
    10321 gccgggcaaa tctcccccgg atatgctgca ccgtgggcag caccaggtgt gcctttaaaa
    10381 gcgattaaat ggatgttcca gcgccatgcg ccgctggcgg ttcgtctcga cggtacgcag
    10441 tttcagttga aatggatgtg gcaaatgtta cgtaactgcg acaccagcca ctacatggaa
    10501 aacaaagggc ggatggtgcg tctggcggaa tacagccgtg attgcctgaa agcattgcgc
    10561 gccgaaacca atattcagta tgaagggcgt cagggtggga cgctgcaact gttccgtacc
    10621 gaacaacagt atgaaaatgc gacccgcgat atcgccgtgc tggaagatgc cggcgtaccg
    10681 tatcagctgc tggaatccag ccgcctggcg gaagtggatc ccgcgctggc agaagtagcg
    10741 cacaaactga cgggcggcct gcagttaccc aatgatgaaa ccggagactg ccagctgttt
    10801 acccagaatc tggcgcggat ggcagagcag gcgggggtta aattccgctt taatacgccc
    10861 gttgaccaac tgctttgcga cggcgagcaa atctacggcg tgaagtttgg cgatgaagtg
    10921 attaaggccg atgcgtatgt gatggcgttt ggttcttact cgacggcgat gctcaaaggc
    10981 attgttgata ttccggtgta tccgctgaaa ggctactcgc tgactattcc aattgcgcag
    11041 gaagatggtg cgccagtatc caccattctt gatgaaacct acaaaatcgc cattacccgt
    11101 ttcgataacc gcattcgtgt tggcggaatg gcggaaattg ttggttttaa caccgagctg
    11161 ttgcaaccgc gtcgtgaaac gctggaaatg gtggttcgcg atctctatcc acgcggcggt
    11221 catgtcgagc aggcgacttt ctggacgggt ttgcgcccga tgacgccaga cggtacgccg
    11281 gttgtcggga gtacacgctt taaaaatctg tggctgaata ccggtcacgg cacgctcggc
    11341 tggacgatgg cttgcggttc cggtcagttg ttaagcgatc tgctctctgg tcgcacgcca
    11401 gcgatcccat atgaggatct aagcgtagcg cgctacagcc gtggatttac gccatcacgt
    11461 ccgggccatt tacatggcgc acacagctaa ggaaacgaga tgacccgtcc gatacaggcc
    11521 agcctcgatc tgcaggcatt aaaacagaat ctgtccattg tccgccaggc cgcgccgcac
    11581 gcgcgcgtct ggtcggtggt aaaagcgaac gcttacgggc atggtattga gcgtatctgg
    11641 agcgcgctcg gggccaccga tggctttgca ttacttaacc tggaagaggc aataacgtta
    11701 cgtgagcgcg gctggaaggg gccgatcctg atgctggaag gatttttcca tgctcaggat
    11761 ctggagattt atgaccagca ccgcctgacc acctgcgtac acagcaactg gcagctcaaa
    11821 gcactgcaaa atgcgcggct aaaagcaccg ttggatattt atcttaaagt gaacagtggg
    11881 atgaatcggt tgggcttcca gtccgatcgc gtgcttaccg tctggcagca gttgcgggca
    11941 atggcgaatg ttggcgaaat gaccctgatg tcgcattttg ctgaagcgga acatcctgat
    12001 ggaatttccg gcgcgatggc gcgtattgag caggcggcgg aggggctgga gtgtcggcgt
    12061 tcgttgtcca attcggcggc gactctgtgg cacccggaag cgcattttga ctgggttcgg
    12121 cctggcatta ttttgtatgg cgcttcgccg tccggtcagt ggcgtgatat cgccaatacc
    12181 ggattacgtc cggtgatgac gctaagcagt gagattattg gtgtccagac gctaaaagcg
    12241 ggcgagcgtg tgggctacgg cggtcgctat actgcgcgcg atgaacagcg aatcggcatt
    12301 gtcgccgcag gatacgccga cggttatccg cgccatgcgc ctaccggtgc tcctgtttta
    12361 gtggacggcg tgcgcaccat gacggtgggg accgtctcga tggatatgct ggcggttgat
    12421 ttaacgcctt gcccgcaggc ggggattggt acgccggttg agctgtgggg caaggagatc
    12481 aaaattgatg atgtcgccgc cgctgccgga acggtgggct atgagttgat gtgcgcactg
    12541 gcgttacgcg tcccggttgt gacagtgtaa cttgttgtaa gccggatcgg aggcagcgtc
    12601 ttctggttgc gaaaaaatca tccatccggc tggtcagcaa ctgtagttgt taatgtgaca
    12661 gagccattgc ccatgatagt gtccattaaa agaatggaca ctatttcccc ggaacctgaa
    12721 ctcgccgcac aggcgttcta cataaaacgc ttacgcttca ttgttgactc ggagtcgact
    12781 tcagataaat cacgcttcac ccttgatgca gattcgggtt catgttcaga ttgttgatta
    12841 acaggctgcg agtcctgttg tctttccaga ctcaacaggc ccaactgttg atccaggagc
    12901 tccaggtcct cctgtacctt cacgcgctcc aggagttccg ggtcatccag gaactccaga
    12961 tccataaggt cctccagctc ctcgagtcct tccaggtcaa cctagccttc cttgtgcaaa
    13021 aagtccaaaa ggtccttcag gccctgctgt tttttcaggc cctcgacggc tttgagcagc
    13081 gtcggtaaat tgtctttgtc ccacgggtta ccattacagt caaacacctt caggctctgg
    13141 ggctgcggca cctgtaccgt tttaaacttg ttttctccac agtagtaaat tattagtttg
    13201 ggcggcagcc gtggtgaaga gatcaaatcg ttactagaac agtcaaatac tttcaggcga
    13261 gtcaagttct ccagcatctc atcaggtatt gagttaatat tgttgccggc acaggatagt
    13321 ttatacaggt actggggcat cgacggcggt aaaaattgca agaagttttc agaacagtta
    13381 atcactctca ggttagtcga cagctcaggc agccagataa tactgttttc atagcacttc
    13441 agtaccatca gttttgaggg ctgcaccttc ggtaacaaga tcaagacgtt atctgtacag
    13501 tcaagcacca gcagccactt tggcatcacc ttcggtaacg cggtcaacac gtttttactg
    13561 cagtaaaatt caatcagatg tggaggcaaa ttctcaggga gtgaagtcaa tcccaggttg
    13621 ctaagattga gcatactccg tcttgtaatg agacattcct tcattctgcg caatgcttct
    13681 gcccggtttt caatccggct tgctggcagg ttttcttcca ggatattttc cttgatttct
    13741 tccagacttt ctttcaggtc ttcttgggga atttctgact gtttgttttg ctggttttct
    13801 tccaggattt cgtacaggtt tttttgcagg ttttcttgca aaccatcctg ctgtatagcc
    13861 cattcgcacc atttggcgac catcatgaac tcgttatgtt gctgattcag acgagtcccc
    13921 aacgtgatga tggtattgtt gtccacctgt atgttcactt tgcccgcgat actgaggtca
    13981 atgatctgcc agggggcatc accccagctg aatttttgcg ggctgagata cttcatgttc
    14041 tgcaaaattt gattcttgag aagagccgcc ctggcgggcg tcgtacgcct gggttaacgg
    14101 aagatggggg aatggcgggg gcgaatgggc agatgtcttt aaagctagcc ataatgctac
    14161 gtttccttga aataaatatc ctctggcagc gccggagacg tgaatgtggt caccgcaaag
    14221 gagccacagg acgtcatttg cgctgatatc actactcatc gttgactttt ttcctggtca
    14281 gaaatgccgt gtcgggtctg gtacatgctg tattacggcc taggtagctc tgacgtatta
    14341 aagaaaatac gagagtgaac gacccggaaa taatcgttat taagcaaagg aataactata
    14401 agaaatcgct catgcatccg gtgctacagg cggatgaaac gcctattgtg tgggctatgt
    14461 cagtgcggca ggttgcgtgg gggaatatgt tcaggcgatg tcaggcaggg gcccctgatg
    14521 gttgaccggt aatgcggggc tggtttttat tacctgttac agtcggaaaa agcccttacg
    14581 ctgactttct tgagccggtg ttttgagttc attagccaga gcctgcaatg gtttgtcatc
    14641 cataatttaa ccttcttctg atgttgggat gagcatatca aaatcaggaa atgacaagtt
    14701 tattacaggc tcaggctgcg ttcagtaaag catcgcatcc ggcgatagat gctgcattaa
    14761 atttaagatt cagcttcctc ttcagccacc cgaacgccaa tcttcaacac ttcattatct
    14821 tctttctcgg ccaccgtcca gatcatcccg gcaaactcta cctggtcacc gacaaccggt
    14881 gccgcgccta gcaactgctg gacaatttca cccagcgttt gctgtttatc acgatattct
    14941 cgcccgtctt ccaggccata tatcagcgca acatcagcat atttggcgct ggcttcgaga
    15001 atgaagtcac caaagaagcg ttgatccagc gcgaccggtg gcgactggct gaacagttta
    15061 ccgagcgccg ggagatcgcg ttcccgacca attacacaca atacatcgcc ttcgcgcagt
    15121 cgggtgctgc cggtaggatg aagcaactgg ttatcacgaa acagggctgc aatacgcgtc
    15181 tcttttggca tatgcaaatc gcgcagtgcc gcacctacac accatttatc agcactcaat
    15241 tgataaacaa actgctccca cggattttcc ggatgaatat ccaggccaac gcgtgacacc
    15301 ggacgtccga cgggcggaac caccactttg gcttttttcg ccgcccacga gagtgatgtt
    15361 ccctgcaaga gcagtgaaac cagaaccaca aagaaggcga cattaaagaa cagtcgcgca
    15421 ttctccagcc ccgccatcat cgggaacacg gctaggatga ttggcaccgc gccgcgtaat
    15481 cccacccagc tgataaacac gcgctcacgc agattgaaac cgcggaaagg gagcaatcca
    15541 gcaaataccg aaaggggacg ggcgaagaat atcatccatg ccgacaaaat gagcgccgga
    15601 atggcaatag gcagcagatc gcttgggtta accaatagcc ccagcaccag gaacatggcg
    15661 atttgcgcca gccaggcgag gccgtcgaaa ttttgcagga tgccgtagcg gttgcgaatc
    15721 ggccgattac ccagcagaaa accgcacaga taaacagcca gaataccgct gccttccagc
    15781 gccgtggtta aagcaaaaat taaaataccg ccgcttaatg ccagcaatgg atataatccg
    15841 gcaggcaggg cgatgcgatt aatcatttgc agcagtaaat aaccaccgcc aagaccgatg
    15901 acaatcccga ggccaaattg ctgcagaata tcgaccacga acatccaact gacactgctt
    15961 tcatgctgct ggatcattgc aatcagggta atcgtcagaa agaccgccat tggatcatta
    16021 ctgccggatt caatttccag cgtcgagcca acacgttcgt taagcccctt accacccagc
    16081 agagaaaaga ccgctgcagc atcggtggag ccgacgatag cgccgattaa taagccttca
    16141 atcaaatcaa gattaaacag ccacgccgcc atcatgccgg ttaaaccaga ggtgataagt
    16201 acgcccagcg tcgccagcga cagcgccggt cctaacgcca cacgaaagga actggcctga
    16261 gtgcgcatcc cgccgtcgag caaaataatc gccagcgcca ggttacttac catgtaggcg
    16321 aaggggtaat tatcaaacgg gataccgccg acgccatcga ctcccgccag catgccgatc
    16381 gccagaaaaa taaccagaat aggaatgcca agacgggaag aaaatgaact aagtaatata
    16441 ctgctggtga caaggatgga tcctaagatg aaaaggctaa ttattgttgt ggcatccaat
    16501 ggtcggttac tcctgattac gctgtctctt atataaaccc taccatatta gcggcagaga
    16561 cagcgtttta cttagccctg aagcgtgatt tttttacatt ttaagaacag gatgaccgct
    16621 aatcgtcaac tgagcgcctt cccgggtgtt attcagaatg gcatgtgcgc ccaacggcag
    16681 cgtaaccgtg cgttgttcat gaccaaaatc gaggccggta atgagtggaa tcgacaggcg
    16741 ggaacgcaaa aacgcgtaca ctgactccag gttgtaacct gcgtcataat cattgggcgt
    16801 gctgccgcta aagctaccga gaataatcgc cttctgacgt ggcaaaattc ccgcatgata
    16861 gagctgcaac agcatacgtt cgacgcggaa agggtgcacg ttaatatctt ccagcaccag
    16921 aataccgttc tcaatttttg gcatccacgg tgtaccaatc agtgaaatca gcatcgcaag
    16981 attgcctccc cacaacgtgc cttcggtctg acatgtcggg ccttcccctt gccattcaat
    17041 ggtgaaggtt tcattgcgta acgccagcca gaaatggtgc tcggtaaagg cgttcagttc
    17101 atccgcacca aagtttgcta ccagcatggg gccgctaaag gtgatgacat tgccttgcgc
    17161 cagaagaccg cactgaatgg cggtaaaatc gctatgtccg caaatgagta acgggtcatg
    17221 ttgttggcga gccaccagtg cctgccagtc aatatctgcc agtaaacgac tggcaccgta
    17281 accgccgcgt acagccagca cgatggtgtt gggggtagtc agtctggcaa gggaattgag
    17341 atcttccaga cgctccgttt ccgtacccgc aaaacgctca caacgacggg caataacctc
    17401 gacgttattg acttgatgcc ccgcgtcggt aaggcgctgg ataccacgca gcgcggcgtg
    17461 ctgtttaatg ctgtaacccg atggggcaat taagtgaaac agagacatgg caattccttg
    17521 ctgacaacag aaacgaaatg tatatcatgc cgcttaggtg tgccgttgtc acctcaacgg
    17581 cgattccagg ctataaggat agaagaagtg aaattgagat ggtttgcctt tttgattgtg
    17641 ttattagcgg gttgttcatc aaagcatgac tatacgaacc cgccgtggaa cgcgaaagtt
    17701 ccggtgcaac gtgcgatgca gtggatgcca ataagccaga aagccggtgc agcctggggc
    17761 gtcgatccac aattgatcac ggcgattatc gctatcgaat cgggtggtaa tccgaacgcg
    17821 gtgagtaaat cgaatgccat tggtttgatg cagttaaaag cttcaacctc cggacgtgat
    17881 gtttatcgcc gtatgggctg gagtggtgag ccgacgacca gcgagctgaa aaatccagag
    17941 cgtaatattt caatgggggc ggcttacctg aatattctgg aaaccggccc gctggcaggc
    18001 attgaagatc cgaaggtact gcaatatgcg ctggtggtgt catacgctaa cggggcaggt
    18061 gcgctgctac ggactttctc gtcagatcgg aaaaaggcga tcagcaaaat caacgatttg
    18121 gatgctgacg agttcctcga tcacgtagcg cgaaatcatc ctgcgccgca ggctccgcgc
    18181 tatatctaca aacttgagca ggcactggac gcgatgtaaa tcagtcgcgc actttgtccg
    18241 ctttttcccg ggcttctcgc tcgagagaga aaataatccg ctgtaattgc cgctccaccg
    18301 tcgggctgac gttaagaaaa cggaagctca gacggggagt ggtgatggtt tcattcttgc
    18361 catcaatcac tttgcgctcg ctggtggaga ttaactgggc gtcaaagtga aaaacacccc
    18421 attgccccat gttgacttca atttgagcga agcgcatacc ttcttgtaat tcggcaggct
    18481 ttgctgtttc cagtaatgcg cccatgccgc ctaacgacaa atcatacaag cggaaacgta
    18541 acgtactgtt atccgccagt ttggtctggc aaaaataagg cggatggagt ggggcggaga
    18601 tgcggaaata tcggcgtcgt tgtacaaacc ataaggtggg aggcggtacg gtaataaatg
    18661 ccggaagctg caagtattca ctctgctgta gttgttcaac agtaaactcg actttcgcac
    18721 cctgagtttc ggcggtaatg gtaatgtgct gtgcctttag cacggcgatg ttgtcttcgg
    18781 cttgactgcc gaaatccagc accagtttat ccggggttat tgccagtatt ttgctgatca
    18841 gctgcccgcc attccaactg agacgcaaag gaattgcggc tttgtgcaaa tcgcgtaaca
    18901 cgcccaggac ggctaacgga ttttgtttca ggaactgctc atggtaatga ctcacgcgga
    18961 aaaactcctg actgacaaac tgtctttggt ttatcggtca tagttaacaa aacttaatac
    19021 aaatgcgtga atatttttta catgttgttc ttaaatcagc cgacaaacgc ccagcatcga
    19081 tcccgccctt gcctatactt agagcgttga cgtaagcatt tcttgcgacc gattcatcga
    19141 aacgagggcc tgaacatggg aattattgcc tggattattt ttggcctgat agccggcatt
    19201 atcggcaagc taatcatgcc ggggcgtgat ggtggtggat ttttcctgac ctgtattctc
    19261 gggatagtcg gtgcggtggt cggcggctgg ctggcgacca tgtttggcat tgacggctcc
    19321 atcagcggtt ttaatttgca cagcttcctg gtggcggtgg tgggcgctat cctggtgctg
    19381 ggcgtattcc gcctcctgcg aagagaataa gattttcata aggcggatag cgatacagat
    19441 gccgctatcc gctttcacat cagaacgtat actcgacacc tgcccagaaa ttgcgacctt
    19501 cttcaataaa cccttcactg taggcatatt gggtatcaaa caggttattg accgacgcat
    19561 tgacgctgaa gccatgacct aaggtgtaat cggctcgaat gtgggtcacc gcaaaaccgg
    19621 cggctttttg tgaaccgtca ctgttgctgt agctggagga acgcgcctct tccgacagcg
    19681 ttacgcttaa cggctcccat ggtttgagtg tcatccatgc ggtcattgtc tgcgttggaa
    19741 gatcggttat cttgccgatg tctttgcgtt tggcgtcagc gtggatcaga gcgtagctca
    19801 atcctacatc cagtatattg ctgattttcc ccttaatacc ggcatccaga ccgctgtaat
    19861 ccaccgtgcc gctgttctga ttttgaatgg tatcggcatt gatattgtgc gagaggatgg
    19921 catcactcac ccggttatag taaacgctga cctcaaatcc ccagtcgcgc gtgaaggcac
    19981 cattccaggt taaatccacc ccgcgagcgc gttccggctt gagctgcggg ttaacaatcg
    20041 ctatctggtt atacgcaggt ttggatgtgg tatagcgttc tttcagcgtc ggaaagcgtg
    20101 tgcggtcata gtacgaaagc gccagagtgt cttcattggc aaagtggtat ttccccatca
    20161 cctgccagtt aaaagctgac tgattgttgt cgtcataatg ggtgatgctg ccatctttct
    20221 cgtgtttttt cgcttctacg ctatcgcgcc agtcatagct gattcccgcc acgacatcga
    20281 cattatcagc agcagcccat tgatattcac tggcgagcga ccaggtacga tcttcatagc
    20341 gatcgtaagc ggcgtgcggc gcaccttttt cccggtgtac gtcatctttc cagttaacgg
    20401 caaacgacag cagatcgttt tcacgcacat cggctgccag ttgtaatccg gcaccgtcgc
    20461 tgtaatcgga ataatggctg tagctgcctt ttttattttt caaatcagcc agcgagttgt
    20521 acatcatcag cgtattttca aaggtgtcgc gatacagccg acttttcagg gtaaaacggt
    20581 cgtttagttg ggtcgctccc tgataataaa aactttcttt gtcatactct ggccactgcc
    20641 agtagcgtga tttttgacca caatttccgc tgtatggcgg gttatctttt tcaccatcct
    20701 gcttaatgta agtcagcgtg tattcatcgt tttcgcgcgg tgtaaaaccg agcttcacaa
    20761 tgccgcgttt atcatcagcc gatgaattaa tcatcttgcc gtgtttgcct gcaatatcat
    20821 tatttacacc atgcggcagg ccgagaaaat cctgctttag ctggctaccg ctgacttgca
    20881 aatacccaag atcgctgctg gcggcaaatg aagcatgcat atcgtaggca ttgtcctgac
    20941 tacggctcca tccctggcga tatcccagac ttgcttcttc agggtggtga tattaatggc
    21001 tccgcccatc tgattaggcc catgaagcag cgacgaatac cctttggaaa cttcgactgc
    21061 ccccagattg ttggtcagaa tccgcgccag atcgaggttg ccgtcatagg ggacataaat
    21121 gggcacaccg tcgaaataga ccggcacctg ccgactatca aagccacgaa cttttacctg
    21181 ttcttcgttg cggctgcctg acttttgcag caccacgccg gggacgacac ttaatgcctg
    21241 agcgacattc tgtttatcca aggccttcat ggtgggttga tcgagaacgg tggtcgtcgt
    21301 cgatgatacc ggactggacc atacggtcag cgtgtcgctc tcctgagccg ctacggcctg
    21361 ctgggtaaag gcaagtgata atgctgtgca gagataacgt tttttgagtc tcatagaata
    21421 aattccgttt aacaaagaat taaatagagg ctgtgggttg cagactgact ttaatgtcag
    21481 ctggtgccgc gtaataaggc gcggaggtaa taaaaaggcg aatgccgcag tcgaggtaat
    21541 ttttgagtgt cgtcagatta attccgccgg tcagagccag cgtgcagtga ggagccagcg
    21601 acggcgctat ttgcgcaatt tcagttgcct gttgcggact aaatttgtcg agctgaagta
    21661 cgtctggttg tgcgcgtaac gcggcgatag cctctttcgg cgtgtcggct tcaacaacaa
    21721 ttttcttctc gggtgcgtgg cgacgtaact gagcgattgc gcctgaccag ccctgattgt
    21781 catgaagaaa atggcggtgg ttggcaaaca ataatatggt ttccgcacat ccggcacgat
    21841 gaattagtcc tccggcagcc agaattgcct gcgaagccag taagcgggtg cccggaattg
    21901 cttttcgggt gcaggcgata ttgccgtcag gataacgttc acgaagcaat gccagcattt
    21961 gagcgagata atcagaaaca ccgcaactcc actccagcac attctggact gccttccatc
    22021 cctgatgaag tgctgcggca ttaccctgcg cacggattag ccgttgaccc gcgttcgctt
    22081 gtgaaccgtc gctgaccgcg tcatcaatag ttaatcccag cgtagttaac atcttacacg
    22141 cgacagaaac gccgctgacg cagccgccct gacggtgaaa aaacgctata tagccaggct
    22201 ggtgtccaat atttaatgct cgcgtggtca ggtcacctcc ctggatatct tccagcagta
    22261 acgcatcgat ttgtgcctga gaaaggaaaa tcatcacagt gcctcctgcg gtacgctatc
    22321 ccaccagatg agcgcccagt cgcggctggc aggggcgagg gcacgagcat cttgttgttg
    22381 ataccagtga tataaccgct cacgctcatc gtcgttaagc gcgccaagac tccagctgac
    22441 gttctgctca aaacgttccc aggtgctgtt atcctgttgg caattttgtc cacggataaa
    22501 atcaacgtgg gcataaattc ccatctgata caggacgttg agcgcaaaga tatagttggg
    22561 taattcgatc acttcccgac cagccgcccg ctgaatggct ggagagacga acgaggtgct
    22621 gaccagatga gttgtataga cgcgaagccg tgcctgatta ttcagtttgc tcattgcatg
    22681 gcgcatatca gccaccagag tggagcggga agccacggca atatcgcagc gtggcaggtc
    22741 actccagtcc tcttcccagg cgcgctggat ccagtggaca ttgtcagcct tcagtgcggc
    22801 ggcgcggcgg gcggcaacgt tcagcatccc ctgactgtaa tcaacgccat aaaccgtagt
    22861 gagtttgtca gccagcgcca gcgatacggt gcctggtccg cagcccatat cgaataaggt
    22921 ctgagcgcct tgcaggtcaa ttttagcgat cagttgttgc aggtagctgt ccgttggcga
    22981 ggcgcaattt tccgccattt tctccgcccg ttgatcccag tgatcgggcg ttttttctgt
    23041 gcggtgagcc agtttgagtt gctgcagata aaggtctgca aaatcaatat catcaatgag
    23101 catgattgtt ccttagcggc taattgcaga gaggtgatga tgaatttggt cggcagaaac
    23161 ccgatacagg gccgccagtt tgttcgttgt taactgttcg gtcggtaagc cttgtgttac
    23221 tcgtccatct ggctctactt ggataatgct gtcggcaatg gcgttggcat ggagcgtatg
    23281 gtgagtcgac attaacattg tcatgccgtt atttttgagc tgcgccagcg tgtccagaag
    23341 ttggatctga tgaccgaaat cgaggctgga ggcgggctca tccagtaaca acaagcgcgg
    23401 ttgttggacg agtgcacggg cgattaaagc cagctgacgt tcaccgccgc taagcgtatt
    23461 ccagcgtcgt gtcgccagat gcaaaatgcc cagcttttcc agttgctccg tggctttcag
    23521 gcgttcttgc ttaccgggaa cggaaaaagc gcctatggtg ggggccaggc ccatcaacac
    23581 catatccagc acactgaaag caaaaatacc gtcgtgcgcc tgcggcaccc aggcaatagc
    23641 ctgtgcgcgc tggcgatggc tgagtgtctg cacagagagg ctatccagta ggatctcgcc
    23701 ttttaataac ggtataacgc caagaatgga gcgcatgagt gtggttttgc cgcaaccatt
    23761 cgcgccaagc agacaacaaa ttgttccttg cgacagggtg aaggacacgt cgcgtagcac
    23821 tggatggcgg gagtaaccca gagacgcatg acgaacggca agcaaagtca ctggcgacct
    23881 ccgcgtaaca acaaaagcag gaaaaacggt gcgccaacaa aagcggtcag aatgccaagt
    23941 gggatttctg ttgttccaat gctgcgcgcc agcgtatcgg taagcaggag cagaatagcg
    24001 ccagtacaca ttgccatagg caacagctgc tgatggttat gaccggtcaa caagcgggca
    24061 atgtgcggaa cgaccagacc gatccaacca attatgcccg ctattgctac cgtgctggcg
    24121 gtgattagtg tggcgcagac aatcaatcca aagcgcagac gcgtcacgtt cagtcccagc
    24181 gaacgggctt cgtcgtcgga aagcgtgagt aaattcatgc gccagcggag aaaaaacagg
    24241 ggcaaggagc ctatgagaat gatcggtgca gcatagcaga gatcgcgtaa ggtcacggtg
    24301 gacagtcccc ccagcaacca gaaggtaatt gacggtaact gggtgtaagg atcggccagc
    24361 gttttaatta gcgatatccc cgctccgcac aacgttccta acgcaatgcc taccagcacc
    24421 agggttaaga tcggatcgtg gcgggtcacc cgacgcgtaa tcaaccagac gcccgcgacc
    24481 accatcagcc cgccgcaaaa tgcgtacagc tggatgtaaa caatgggtaa tccccacagg
    24541 attgccgcgc aggcaccaag tccggcaccg gcggagacgc ctaaaatatc cggtgaaact
    24601 aagggattac gcaacattcc ctgataggtg gttccggctc ctgccagcgc tgcgcccagt
    24661 aacagcgcag caagaatacg cggcacgcgg atttgccaga agacaatttg ttcctgcaca
    24721 gactcatttt cttgacgaag aattagcgcc agcagttgct gcatatcgag atgataagcc
    24781 ccacttacgg cggcaaaagt gatactgata gctgccagca gcaaaataca tccgtttacg
    24841 attctcatta gctcgccacc aatttttgat agtcggcatc actgagtgaa caatgccaga
    24901 acagttgggc gtaatgctgc atgtcacttt taaactggcg attgatggcg ggatccagcc
    24961 aggcgtgaag tctgcgtaat cccagaagac ggttgattcc cggcggggca tcgagccagc
    25021 caaagggcaa gccacttaaa aataagatgc gttgttccgc aacggctttc acgccctgcc
    25081 agagaggatc acgacgaata aaatctgcag taacggcctc ctgaaccaga ataatatccg
    25141 gctgccagcg caggagattt tccatggaaa cctgtgtcag gccgtggcga tcggctattt
    25201 gcgcgacgtt gtgcaaacct aataattccg ccgcctcggt atgcaacgaa ccctgtaagc
    25261 ccgtttccag ccctcgggga ccgcgcgcag cataaaagcg gaggttagcg gcgggtgagg
    25321 tggcgaatgc ctgagcttca ccaacgaagt ggctggcgag attggcttgt tctgcggcgc
    25381 ggtgctcttc gcctaacgtt ttgccaagcg ttgttaactg ttctgctgat tgcgccagtt
    25441 ttccgttaag caataaccag gggatttgtg tctgttcgct aacctgccgt gcctgggaga
    25501 tcaaggtttc atccgtattg ccgcaatcaa cgaccaaatc gggatgtaac gccataagcc
    25561 cttccagcga tagtgtgctg gcgcgcccgg cgagtcgtcc cagcctgggt aactggcgaa
    25621 tgtgctctgg caatggaatt aatgcctgac gggcaaaatc aaaagaggaa aaaccgacca
    25681 ttttttctgg cgcgacagcc agcaagagca aatcggcggg cgcgcctgcg ctgacgatgc
    25741 gtgtaatgtt ttgggcttcg ggaagagatt gcgaagcaaa acgattaact gtttgtgaaa
    25801 aggacggtag agactgcagg agcagggtag atgcaagtgc ctgcgcaaac gtccgtctgg
    25861 ttatgggcat ggtgaacatc ctgaaaagta aaatcgatat atactttcat atatatcgat
    25921 aaaaatcatg ttcaatatca aagtttagct attgaaaaca tgaagatgtg acgacttccg
    25981 caagcctgtt gaccctctct atcattaaaa atgtatttat caggtggcta atattattta
    26041 aatcatttaa ggctaaatta tttaaatatg aatgacatta ttgttttttt atcgtccacg
    26101 gttacgcgac gttgaagtta cttgttttat aatgagcgat ttttgatatc tcgccgagaa
    26161 aattatcagc attctctcgt ttcagtgtcg tgaatatact gttaacggag gtgaaaagaa
    26221 tgctgggggt tatacaaaac ctatgttgtt gtcgtccgga ttcaccgctt gctgatagta
    26281 agcagcgtaa ccagaagtta ccgcaacaag
//
LOCUS       DAJTRC010000056        25143 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.056, whole genome shotgun sequence.
ACCESSION   DAJTRC010000056 DAJTRC010000000
VERSION     DAJTRC010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 25143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25143
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.056"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="OO357_002446"
     CDS             complement(<1..1358)
                     /gene="yeaG"
                     /locus_tag="OO357_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase YeaG"
                     /protein_id="HCR5887360.1"
                     /translation="MNIFDHYRQRYEAAKDEEFTLQEFLTTCRQDRSAYANAAERLLM
                     AIGEPVMVDTAQDPRLSRLFSNRVIARYPAFEEFYGMEDAIEQIVSYLKHAAQGLEEK
                     KQILYLLGPVGGGKSSLAERLKSLMQLVPIYVLSANGERSPVNDHPFCLFNPQEDAQI
                     LEKEYGIPRRYLGTIMSPWAAKRLHEFGGDITKFRVVKVWPSILQQIAIAKTEPGDEN
                     NQDISALVGKVDIRKLEHYAQNDPDAYGYSGALCRANQGIMEFVEMFKAPIKVLHPLL
                     TATQEGNYNGTEGISALPFNGIILAHSNKSEWVTFRNNKNNEAFLDRVYIVKVPYCLR
                     ISEEIKIYEKLLNHSELTHAPCAPGTLETLSRFSILSRLKEPENSSIYSKMRVYDGES
                     LKDTDPKAKSYQEYRDYAGVDEGMNGLSTRFAFKILSRVFNFDHVEVAANPVHLFYVL
                     EQ"
     gene            1794..2540
                     /gene="mipA"
                     /locus_tag="OO357_002447"
     CDS             1794..2540
                     /gene="mipA"
                     /locus_tag="OO357_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein MipA"
                     /protein_id="HCR5887361.1"
                     /translation="MTKLKLLALGVLIATSAGVAHAEGKFSLGAGVGVVEHPYKDYDT
                     DVYPVPVINYEGDNFWFRGLGGGYYLWNDATDKLSITAYWSPLYFKAKDSGDHQMRHL
                     DDRKSTMMAGLSYAHFTQYGYLRTTLAGDTLDNSNGIVWDMAWLYRYTNGGLTVTPGI
                     GVQWNSENQNEYYYGVSRKESARSGLRGYNPNDSWSPYLELSASYNFLGDWSVYGTAR
                     YTRLSDEVTDSPMVDKSWTGLISTGITYKF"
     gene            2630..3391
                     /locus_tag="OO357_002448"
     CDS             2630..3391
                     /locus_tag="OO357_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HCR5887362.1"
                     /translation="MQQKMIQFSGDVSLPAIGQGTWYMGEDASQRKTEVAALRAGIEL
                     GLTLIDTAEMYADGGAEKVVGEALTGLREKVFLVSKVYPWNAGGQKAINACEASLRRL
                     NTDYLDLYLLHWSGSFAFEETVAAMEKLIAQGKIRRWGVSNLGYADMQELWQLPGGNQ
                     CATNQVLYHLGSRGIEYDLLPWCQQQQMPVMAYSPLAQAGRLRNGLLKNAVVNEIAHV
                     QQHAAALEVELSSAELAMLDKAYPAPKGKTALDMV"
     gene            complement(3442..4326)
                     /locus_tag="OO357_002449"
     CDS             complement(3442..4326)
                     /locus_tag="OO357_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-hexose-6-phosphate mutarotase"
                     /protein_id="HCR5887363.1"
                     /translation="MIKKIFALPVIEQISPVLSRRKLDELDLIVVDHPQVKASFALQG
                     AHLLSWKPAGEEEVLWLSNNTPFKNGVAIRGGVPVCWPWFGPAAQQGLPAHGFARNLP
                     WTLKSHHEDADGVALTFELTQSEETKKFWPHDFTLLAHFRVGKTCEIDLESHGEFETT
                     SALHTYFNVGDIAKVSVSGLGDRFIDKVNDAKEDVLTDGIQTFPDRTDRVYLNPQDCS
                     VINDEALNRIIAVGHQHHLNVVGWNPGPALSVSMGDMPDDGYKTFVCVETAYASETQK
                     VTKEKPAHLAQSIRVAKR"
     gene            complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="OO357_002450"
     CDS             complement(4410..5405)
                     /gene="gapA"
                     /locus_tag="OO357_002450"
                     /EC_number="1.2.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HCR5887364.1"
                     /translation="MTIKVGINGFGRIGRIVFRAAQKRSDIEIVAINDLLDADYMAYM
                     LKYDSTHGRFDGTVEVKDGHLIVNGKKIRVTAERDPANLKWDEVGVDVVAEATGLFLT
                     DETARKHITAGAKKVVMTGPSKDNTPMFVKGANFDKYAGQDIVSNASCTTNCLAPLAK
                     VINDNFGIIEGLMTTVHATTATQKTVDGPSHKDWRGGRGASQNIIPSSTGAAKAVGKV
                     LPELNGKLTGMAFRVPTPNVSVVDLTVRLEKAATYEQIKAAVKAAAEGEMKGVLGYTE
                     DDVVSTDFNGEVCTSVFDAKAGIALNDNFVKLVSWYDNETGYSNKVLDLIAHISK"
     gene            5747..6160
                     /gene="msrB"
                     /locus_tag="OO357_002451"
     CDS             5747..6160
                     /gene="msrB"
                     /locus_tag="OO357_002451"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HCR5887365.1"
                     /translation="MANKPSAEELKKNLSEMQFYVTQNHGTEPPFTGRLLHNKRDGVY
                     HCLICDAPLFHSETKYDSGCGWPSFYEPVSEESIRYIKDLSHGMQRIEIRCGNCDAHL
                     GHVFPDGPQPTGERYCVNSASLRFTDGENGEEING"
     gene            6202..6474
                     /locus_tag="OO357_002452"
     CDS             6202..6474
                     /locus_tag="OO357_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeaC family protein"
                     /protein_id="HCR5887366.1"
                     /translation="MNLDDIINSMTPEVYQRLSTAVELGKWPDGVALTEEQKENCLQL
                     VMLWQARHNTEAQHMTIDTNGQMVMKSKQQLKEDFGISAKPIAMFK"
     gene            6844..7920
                     /locus_tag="OO357_002453"
     CDS             6844..7920
                     /locus_tag="OO357_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HCR5887367.1"
                     /translation="MKALARFGKAFGGYKMIHVPQPICGPEDVVTEIKAAAICGADMK
                     HYNVDSGSDEFNSIRGHEFAGCIAQVGEKVKDWKVGQRVVSDNSGHVCGVCPACEQGD
                     FLCCTEKVNLGLDNNTWGGGFSKYCLVPGEILKIHRHALWEIPDGVDYEDAAVLDPIC
                     NAYKSIAQQSKFLPGQDVVVIGTGPLGLFSVQMARIMGAVNIVVVGLQEDVAVRFPVA
                     KELGATAVVNGSTEDVVARCQQICGKDNLGLVIECSGANIALKQAIEMLRPNGEVVRV
                     GMGFKPLDFSINDITAWNKSIIGHMAYDSTSWRNAIRLLASGAIKVKPMITHRIGLSQ
                     WREGFDAMVDKTAIKVIMTYDFDE"
     gene            7947..9326
                     /locus_tag="OO357_002454"
     CDS             7947..9326
                     /locus_tag="OO357_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5887368.1"
                     /translation="MEQITKPHCGARLDRLPDCRWHSSMFAIVAFGLLVCWSNAVGGL
                     ILAQLKALGWTDNSTTATFSAITTAGMFLGALVGGIIGDKTGRRNAFILYEAIHIASM
                     VVGAFSPNMDFLIACRFVMDVGLGALLVTLFAGFTEYMPGRNRGTWSSRVSFIGNWSY
                     PLCSLIAMGLTPLISAEWNWRVQLLIPAILSLIATALAWRYFPESPRWLESRGRYQEA
                     EKVMRSIEEGVIRQTGKPLPPVVIADDGKAPQAVPYSALLTGVLLKRVILGSCVLITM
                     NVVQYTLINWLPTIFMTQGINLKDSIVLNTMSMFGAPFGIFIAMLVMDKIPRKTMGVG
                     LLILIAVLGYIYSLQTSMLLITLIGFFLITFVYMYVCYASAVYVPEIWPTEAKLRGSG
                     LANAVGRISGIAAPYAVAVLLSSYGVTGVFILLGAVSNIVAIAIATIGIETKGVSVES
                     LSIDAVANK"
     gene            9343..10388
                     /locus_tag="OO357_002455"
                     /pseudo
     CDS             9343..10388
                     /locus_tag="OO357_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416288.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
     gene            10409..11245
                     /locus_tag="OO357_002456"
     CDS             10409..11245
                     /locus_tag="OO357_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HCR5887369.1"
                     /translation="MLADIRYWENDATNKYYAIAHFNVWNAEMLMGVKDAAEEAKSPV
                     IISFSTGFVGNTSFEDFSHMMVSMAQKATVPVITHWDHGRSIEIIHNAWTHGMNSLMR
                     DASAFDFEENIRLTKEAVDFFHPLGIPVEAELGHVGNETVYEEALAGYHYTDPDQAAE
                     FVERTGCDSLAVAIGNQHGVYTSEPQLNFEVVKRVRDAVSVPLVLHGASGISDADIKT
                     AISLGIAKINIHTELCQAAMVAVKENQDQPFLHLEREVRKAVKERALEKIELFGSDGK
                     AE"
     gene            11250..12197
                     /locus_tag="OO357_002457"
     CDS             11250..12197
                     /locus_tag="OO357_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707341.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HCR5887370.1"
                     /translation="MDNLDVICIGAAIVDIPLQPVSKNIFDVDSYPLERIAMSTGGDA
                     INEATIISRLGHRTALMSRIGKDASGQFILDHCRKENIDIQSLKQDVSIDTSINVGLV
                     TEDGERTFVTNRNGSLWKLNIDDVDFARFPQAKLLSLASIFNSPLLDGKALTEIFTQA
                     KARQMIICADMIKPRLNETLDDICEALSYVDYLFPNFAEEKLLTGKETLDEIADCFLA
                     CGVKTVVIKTGKDGCFIKRGDMTMKVPAVAGITAIDTIGAGDNFASGFIAALLEGKNL
                     RECARFANATAAISVLSVGATTGVKNRKLVEQLLEEYEG"
     gene            12207..13187
                     /gene="ydjG"
                     /locus_tag="OO357_002458"
     CDS             12207..13187
                     /gene="ydjG"
                     /locus_tag="OO357_002458"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent methylglyoxal reductase"
                     /protein_id="HCR5887371.1"
                     /translation="MKKIPLGTTDITLSRMGLGTWAIGGGPAWNGDLDRQICIDTILE
                     AHRCGINLIDTALGYNFGNSEVIVGQALKKLPREQVVVETKCGIVWERKGSLFNKVGD
                     RQLYKNLSPESIREEVEASLQRLGIDYIDIYMTHWQSVPPFFTPIAETVAVLNELKAE
                     GKIRAIGAANVDADHIREYLQYGELDIIQAKYSILDRAMENELLPLCRDNGIVVQVYS
                     PLEQGLLTGTITRDYVPGGARANKVWFQRENMLKVIDMLEQWQPLCARYQCTIPTLAL
                     AWILKQSDLISILSGATAPEQVRENVAALNINLSDADATLMREMAEALER"
     gene            13324..14082
                     /locus_tag="OO357_002459"
     CDS             13324..14082
                     /locus_tag="OO357_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5887372.1"
                     /translation="MAAKDRIQAIKQMVANDKKVTVSNLSGIFQVTEETIRRDLEKLE
                     DEGFLTRTYGGAVLNTAVLTENIHFYKRASSFYEEKLLIARKALPFIDNKTTMAADSS
                     STVMELLKLLQDRSDLTLLTNSAEAIHVLAQSEIKVVSTGGELNKNTLSLQGRITKEI
                     ISRYHVDIMVMSCKGLDINSGALDSNEAEAEIKKTMIRQATEVALLVDHSKFDRKAFV
                     QLADFSHINYIITDKSPGAEWIAFCKDNNIQLVW"
     gene            14200..15558
                     /locus_tag="OO357_002460"
     CDS             14200..15558
                     /locus_tag="OO357_002460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5887373.1"
                     /translation="MEQYDQIGARLDRLPLARFHYRIFGIISFSLLLTGFLSYSGNVV
                     LAKLVSNGWSNNFLYAAFTSALMFGYFIGSLTGGFIGDYFGRRRAFRINLLIVGIAAT
                     GAAFVPDMYWLIFFRFLMGTGMGALIMVGYASFTEFIPATVRGKWSARLSFVGNWSPM
                     LSAAIGVVVIAFFSWRIMFLLGGIGILLAWFLSGKYFIESPRWLEGKGQIAGAESQLR
                     EVEQQIEREKSIRLPQLTLNQSNSKVKVIKGTFRLLFKGEMLRRTLVAITVLIAMNIS
                     LYTITVWIPTIFVNSGIDVDKSILMTAVIMIGAPVGIFIAALIIDHFPRRLFGSALLI
                     IIAVLGYIYSSQTTEWAILIYGLVMIFFLYMYVCFASAVYIPELWPTHLRLRGSGFVN
                     AVGRIVAVFTPYGVAALLTHYGSITVFMVLGVMLVLCALVLSIFGIETRKVSLEEISE
                     VN"
     gene            complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="OO357_002461"
     CDS             complement(15651..16292)
                     /gene="pncA"
                     /locus_tag="OO357_002461"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310502.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HCR5887374.1"
                     /translation="MPHRALLLVDLQNDFCAGGALAVPEGDSTVEVANRLIDWCQSRG
                     EAVIASQDWHPANHGSFASQHGVEPYTPGQLGGLRQTFWPEHCVQNSEGAQLHPLLNQ
                     KEIAAVFHKGENPLVDSYSAFFDNGRRQKTALDDWLRDHEIDELIVMGLATDYCVKFT
                     VLDALQLGYKVNVITDGCRGVNIQPQDSAHAFMEMSAAGATLYTLADWEETQG"
     gene            complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="OO357_002462"
     CDS             complement(16303..17319)
                     /gene="ansA"
                     /locus_tag="OO357_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HCR5887375.1"
                     /translation="MQKKSIYVAYTGGTIGMQRSEQGYIPVSGHLQRQLALMPEFHRP
                     EMPDFTIHEYTPLMDSSDMTPEDWQHIAEDIKAHYDDYDGFVILHGTDTMAYTASALS
                     FMLENLGKPVIVTGSQIPLAELRSDGQINLLNALYVAPNYPINEVTLFFNNRLYRGNR
                     TTKAHADGFDAFASPNLPPLLEAGIHIRRLNTPPAPHGEGELIVHPITPQPIGVVTIY
                     PGISADVVRNFLRQPVKALILRSYGVGNAPQNKAFLQELQEASDRGIVVVNLTQCMSG
                     KVNMSGYATGNALAHAGVIDGADMTVEATLTKLHYLLSQELDTETIRKAMSQNLRGEL
                     TPDD"
     gene            complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="OO357_002463"
     CDS             complement(17486..19342)
                     /gene="sppA"
                     /locus_tag="OO357_002463"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HCR5887376.1"
                     /translation="MRTLWRFIAGFFKWTWRLLNFVREMVLNLFFIFLVLVGVGIWMQ
                     VSGGDSKETASRGALLLDISGVIVDKPDSSQRFSKLSRQLLGASSDRLQENSLFDIVN
                     TIRQAKDDRNITGIVMDLKNFAGGDQPSMQYIGKALKEFRDSGKPVYAVGENYSQGQY
                     YLASFANKIWLSPQGVVDLHGFATNGLYYKSLLDKLKVSTHVFRVGTYKSAVEPFIRD
                     DMSPAAREADSRWIGELWQNYLNTVAANRQIPAQQVFPGAQGLLEGLTKTGGDTAKYA
                     LENKLVDALASSAEIEKALTKEFGWSKTDKNYRAISYYDYALKTPADTGDSIGVVFAN
                     GAIMDGEETQGNVGGDTTAAQIRDARLDPKVKAIVLRVNSPGGSVTASEVIRAELAAA
                     RAAGKSVVVSMGGMAASGGYWISTPANYIVANPSTLTGSIGIFGVITTVENSLDSIGV
                     HTDGVSTSPLADVSITRALPPEAQQMMQLSIENGYKRFITLVADARHSTPEQIDKIAQ
                     GHVWTGQDAKANGLVDSLGDFDDAVAKAAELAKVKQWHLEYYVDEPTFFDKVMDNMSG
                     SVRAMLPDAFQAMLPAPLASVASTVKSESDKLAAFNDPQNRYAFCLTCANVR"
     gene            19503..20054
                     /locus_tag="OO357_002464"
     CDS             19503..20054
                     /locus_tag="OO357_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H nitroreductase"
                     /protein_id="HCR5887377.1"
                     /translation="MDALELLINRRSASRLAEPAPTGEQLQNILRAGMRAPDHKSMQP
                     WHFFVIEGEGCERFSAVLEQGAIAAGSDDKAIDKARNAPFRAPLIITVVAKCKENHKV
                     PRWEQEMSAGCAVMAMQMAAVAQGFGGIWRSGALTESPVVREAFGCREQDKIVGFLYL
                     GTPQLKASTSINVPDPTPFVTYF"
     gene            20171..21214
                     /gene="selD"
                     /locus_tag="OO357_002465"
     CDS             20171..21214
                     /gene="selD"
                     /locus_tag="OO357_002465"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707349.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HCR5887378.1"
                     /translation="MSENSIRLTQYSHGAGCGCKISPKVLETILHSEQAKFVDPNLLV
                     GNETRDDAAVYDLGNGTSVISTTDFFMPIVDNPFDFGRIAATNAISDIFAMGGKPIMA
                     IAILGWPINKLSPEIAREVTEGGRYACRQAGIALAGGHSIDAPEPIFGLAVTGIVPTE
                     RVKKNSTAQAGCKLFLTKPLGIGVLTTAEKKSLLKPEHQGLATEVMCRMNIAGASFAN
                     IEGVKAMTDVTGFGLLGHLSEMCQGAGVQARVDYEAIPKLPGVEEYIKLGAVPGGTER
                     NFASYGHLMGEMPREVRDLLCDPQTSGGLLLAVMPEAENEVKTTAAEFGIELTAIGEL
                     VPARGGRAMVEIR"
     gene            21219..23180
                     /gene="topB"
                     /locus_tag="OO357_002466"
     CDS             21219..23180
                     /gene="topB"
                     /locus_tag="OO357_002466"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HCR5887379.1"
                     /translation="MRLFIAEKPSLARAIADVLPKPHRKGDGFIECGNGQVVTWCIGH
                     LLEQAQPDAYDSRYARWNLADLPIVPEKWQLQPRPSVTKQLNVIKRFLHEASEIVHAG
                     DPDREGQLLVDEVLDYLQLAPEKRQQVQRCLINDLNPQAVERAIDRLRSNSEFVPLCV
                     SALARARADWLYGINMTRAYTILGRNAGYQGVLSVGRVQTPVLGLVVRRDEEIENFVA
                     KDFFEVKAHIVTPADERFTAIWQPSEACEPYQDEEGRLLHRPLAEHVVNRISGQPAIV
                     TSYNDKRESESAPLPFSLSALQIEAAKRFGLSAQNVLDICQKLYETHKLITYPRSDCR
                     YLPEEHFAGRHAVMNAISVHAPDLLPQPVVDPDIRNRCWDDKKVDAHHAIIPTARSSA
                     INLTENEAKVYNLIARQYLMQFCPDAVFRKCVIELDIAKGKFVAKARFLAEAGWRTLL
                     GSKERDEENDGTPLPVVAKGDELLCEKGEVVERQTQPPRHFTDATLLSAMTGIARFVQ
                     DKDLKKVLRATDGLGTEATRAGIIELLFKRGFLTKKGRYIHSTDAGKALFHSLPEMAT
                     RPDMTAHWESVLTQISEKQCRYQDFMQPLVGTLYQLIDQAKRTPVRQFRGIVAPGSGG
                     SADKKKAAPRKRSAKKSPPADEAGSGAIA"
     gene            23307..24347
                     /locus_tag="OO357_002467"
     CDS             23307..24347
                     /locus_tag="OO357_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1266 domain-containing protein"
                     /protein_id="HCR5887380.1"
                     /translation="MKKVLLQNHPGSEKYSFNGWGIFNSNFERVIKENKAMLLCKWGF
                     YLTCVVAVMFVFAAITSNGLNERGLITAGCSFLYLLIMMGLIVRAGFKAKKEQLHYYQ
                     AKGIEPLSIEKLQALQLIAPYRFYHKQWSETLEFWPRKPEPGKDTFQYHVLPFDSIDI
                     ISKRRESLENQWGIEDSESYCALMEHFLSGDHGANTFKANMEEAPEQVIALLNKFAVF
                     PSDYISDCANNRSGKSSAKLIWAAELSWMISISSTAFQNGTIEEELAWHYIMLASRKA
                     HELFESEEDYQKNSLMGFLYWHICCYRRKLTDAELEACYRYDKQFWEHYSKKCRWPIR
                     NVPWGASSVKYS"
     gene            complement(24464..25126)
                     /locus_tag="OO357_002468"
     CDS             complement(24464..25126)
                     /locus_tag="OO357_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005074866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCR5887381.1"
                     /translation="MGFEGMSVSVSGSGNVAQYAIEKAMEFGARVITASDSSGTVVDE
                     SGFTKEKLARLIEIKASRDGRAADYAKEFGLVYLEGQQPWSVPVDIALPCATQNELDV
                     DAAHQLIANGVKAVAEGAYMPTTIEATELFQQAGVLFAPGKAANAGGVATSGLEMAQN
                     AARLGWKAEKVDARLHHIMLDIHHACVEHGGEGEQTNYVQGANIAGFVKVADAMLAQG
                     VI"
BASE COUNT         6391 a         5824 c         6496 g         6432 t
ORIGIN      
        1 tgctgttcca ggacgtagaa cagatggacc gggtttgccg ccacttctac atgatcgaag
       61 ttgaacacgc gggagaggat cttaaacgca aaacgcgtcg acagaccgtt catcccttca
      121 tcgacaccgg cgtagtcacg atattcctga tacgacttgg ctttcggatc ggtgtctttc
      181 agactttcgc catcataaac ccgcatcttt gaataaatgc tggagttttc tggctctttc
      241 aggcgcgaaa gaatggaaaa acgtgacagt gtttcgagcg tgccaggggc gcatggggcg
      301 tgagtcaatt cactgtgatt aagcaatttc tcgtagattt tgatctcttc ggaaatgcgc
      361 aagcaatacg gcaccttcac gatgtaaaca cggtcgagga aggcttcgtt gtttttgtta
      421 ttacggaaag tgacccattc ggacttgttc gagtgggcga gaataatccc gttgaacggc
      481 agggcggaga taccttccgt cccgttgtag ttaccttcct gagtggcggt taacaaggga
      541 tgcagcactt ttatcggtgc tttaaacatc tcaacgaatt ccatgatccc ctgattggcg
      601 cggcacagcg caccggaata gccgtaggcg tccgggtcat tctgcgcgta gtgttcgagt
      661 ttacgaatat cgactttccc aaccagcgcg gagatgtcct ggttgttctc atcaccgggt
      721 tccgttttgg cgatagcaat ttgttgcaga attgacggcc agaccttcac tacccggaac
      781 ttagtgatat cgccaccaaa ttcatgcagg cgttttgccg cccatggcga catgatggtg
      841 ccgagataac ggcgaggaat gccatactct ttttccagaa tctgagcatc ttcctgcgga
      901 ttgaaaagac agaacggatg atcgttgacc gggctacgct caccgttggc gctcaataca
      961 taaatcggta cgagctgcat taatgatttc agtcgctcag caagcgatga tttaccccca
     1021 cccacaggcc ccagcagata caggatttgt ttcttctctt ccagcccctg agccgcgtgt
     1081 ttcagataag agacaatctg ttcaatcgcg tcttccatgc cgtaaaactc ttcaaatgcc
     1141 ggataacgtg caatgacccg gttagaaaag agtcgagaaa gtctggggtc ctgggctgta
     1201 tcgaccatga caggctcacc gatagccatc aatagccgct cagccgcgtt ggcataagca
     1261 ctgcgatctt gccgacaagt ggtaagaaac tcctgcagtg tgaactcttc gtccttggca
     1321 gcttcatatc gctggcgata gtgatcgaat atattcatgg tatgccgtcc tttcgttttt
     1381 tagcacaggt taagagccgt tcatatgaat agtagaggct cccggaagag gtaaactgaa
     1441 cgacgtctct tgctaaaaca cagcaactct catgccattt tatcgacgat gagaacaacg
     1501 tgcccggtga tacaccttca aaattaagcg tagatggcaa atggaaaact tgcctgcacg
     1561 gctttcttaa tttcaatggc atatcaacaa ctcatccata aattttgcat aattaatgta
     1621 aagaccaggc tcgccagtaa cgctaaatcc atttggctgt aagcgcggtg tcatccgcgg
     1681 caggaaaatt aaacagttac tttaaaaaat gaaaacgtaa aaaggttggg tttcgatgta
     1741 ttgacgggta aactttgtcg cccgctaaac atttgtttaa ggaatgatta attgtgacca
     1801 aactcaaact tctggcactt ggagtgctta tcgcaacgtc tgcaggcgta gcgcacgctg
     1861 aaggtaaatt ttccctgggc gcaggcgtag gtgtcgttga gcacccatat aaagattacg
     1921 ataccgatgt ttacccagta ccggtaatca actatgaagg cgataacttc tggttccgtg
     1981 gcttaggtgg tggttactac ctgtggaatg acgcaacgga taaactttca attaccgctt
     2041 actggtcgcc gctttacttc aaagcgaaag acagtggcga tcaccaaatg cgtcacctgg
     2101 atgaccgtaa gagcaccatg atggctggtc tgtcttatgc tcactttacc cagtacggtt
     2161 acctgcgtac caccctggct ggcgataccc tggataacag caacggcatt gtctgggata
     2221 tggcctggtt gtatcgttac accaacggtg gcctgaccgt gactccgggt attggtgtgc
     2281 agtggaacag cgaaaaccag aacgaatact attatggcgt atcgcgcaaa gagtccgctc
     2341 gcagcggtct gcgtggctat aacccgaatg acagctggag cccttacctt gagctgagcg
     2401 ccagctacaa cttcctcggc gactggagtg tttacggtac cgcgcgctac acccgtctgt
     2461 ctgatgaagt tactgacagc ccgatggtgg ataaatcctg gactggcctg atttctaccg
     2521 ggatcaccta caaattctga taatgcatct ttccagggca ttttgtgcat aaaaacaggg
     2581 cgtcacgccc tgttttgcat tacggtggtg caatacctgg gaggaaacaa tgcaacaaaa
     2641 aatgattcaa tttagtggcg atgtctcact gccagccata gggcagggaa catggtatat
     2701 gggcgaagat gccagtcagc gcaaaacaga agttgctgca ctacgcgcag gcattgaact
     2761 cggtttaacc ctcattgata ccgccgaaat gtatgccgat ggcggtgccg aaaaggtggt
     2821 tggggaagca ttaaccggtc tgcgagagaa ggtctttctc gtctctaaag tctatccgtg
     2881 gaatgctggc gggcaaaaag cgataaatgc atgcgaagcc agtttacgcc gtctcaatac
     2941 tgactatctc gatctctact tattacactg gtctggcagt ttcgcttttg aagagactgt
     3001 tgctgcgatg gaaaaattga tcgcccaggg aaaaatccgt cgctggggcg tttctaacct
     3061 tggttatgcc gatatgcagg aactctggca gctgccgggg ggaaatcagt gtgccactaa
     3121 tcaggtgctt taccatctcg gttcacgagg gattgagtac gatctactcc cctggtgcca
     3181 gcaacagcag atgccggtga tggcttacag tccattagcc caggccgggc ggttgcgcaa
     3241 tggactgtta aaaaacgcgg tagtcaacga aattgcacat gtccaacaac atgcagctgc
     3301 gcttgaggtc gaactttcgt cagcggaatt agctatgctg gataaggcat atccggcacc
     3361 aaaaggaaaa actgcgctgg atatggtgtg atgttgatac gtgccggatg catcgcaccc
     3421 ggcacacaac attaacgcaa attaacgttt ggcaacgcga atggattgcg ccagatgtgc
     3481 aggtttctct ttggtgactt tttgcgtttc tgaagcgtaa gccgtttcta cacaaacaaa
     3541 ggtcttgtag ccatcgtccg gcatgtcgcc catgctaact gaaagcgccg gtcccgggtt
     3601 ccagccgaca acgttcagat gatgctggtg gcctacggcg ataatacgat tcagcgcttc
     3661 atcattaatc acgctgcaat cttgcggatt cagatacacg cgatcggtac ggtcagggaa
     3721 ggtctgaata ccgtcggtca gtacatcttc tttcgcgtca ttcactttat caatgaagcg
     3781 atcgcccagc ccactgacgc ttaccttagc gatatcaccc acgttaaagt aggtatgcag
     3841 ggcagaggtg gtttcaaatt cgccatgtga ttcaagatcg atttcacagg ttttacccac
     3901 gcggaaatgc gctaacagcg taaagtcgtg cggccagaat tttttcgtct cttcgctttg
     3961 tgtcaattca aaagtcagcg ctacgccatc agcatcttca tgatgtgatt tcagcgtcca
     4021 cggcaggttg cgggcaaaac cgtgcgcagg cagaccttgt tgtgccgccg gaccaaacca
     4081 cggccagcaa accggtacgc caccgcgaat agcgacgcca tttttgaacg gtgtgttgtt
     4141 gctcaaccac agaacttctt cttcacccgc aggtttccac gagagaaggt gtgcgccctg
     4201 taatgcaaaa gaggctttta cctggggatg atcgaccaca atgaggtcca gttcatccag
     4261 tttacgacgg gagaggacag gggagatttg ttcgatgacc ggaagggcaa aaattttctt
     4321 aatcatgacg cagtccttta acttcatttt atcaggtaaa aaaaagagcg accgaagtcg
     4381 ctctttttag atcacagtgt catctcaact tatttggaga tgtgagcgat caggtccaga
     4441 actttgttgg agtaaccggt ttcgttgtcg taccaggata ccagtttcac gaagttgtcg
     4501 ttcagagcga taccagcttt agcatcgaac acggaagtgc aaacttcgcc gttgaaatcg
     4561 gtagatacta cgtcatcttc ggtgtagccc agaacgcctt tcatttcgcc ttcagcagca
     4621 gctttaacgg cagctttgat ctgctcgtaa gttgcagctt tttccagacg aacggtcagg
     4681 tcaactacgg atacgttcgg ggtcggaacg cggaacgcca taccagtcag tttgccattc
     4741 agttctggca gtactttacc tacagcttta gcagcaccgg tagaggacgg gatgatgttc
     4801 tgggaagcgc cgcggccgcc gcgccagtct ttgtgagacg ggccatcaac ggttttctga
     4861 gtagcggtag tagcgtgaac ggtggtcatc agaccttcga tgatgccgaa gttatcgttg
     4921 ataactttag ccagcggagc caggcagttg gtggtgcagg aagcgttgga aacgatgtcc
     4981 tggccagcat atttgtcgaa gttagcgcct ttaacgaaca tcggagtgtt gtctttagac
     5041 ggaccagtca taaccacttt cttcgcacca gcggtgatgt gtttacgagc agtttcgtca
     5101 gtcaggaaca gaccagttgc ttcagcgaca acgtcaacac ccacttcgtc ccatttcagg
     5161 ttagccggat cacgttcagc ggtaacacgg atttttttac cgttaacgat cagatgaccg
     5221 tctttcactt caacggtacc gtcgaaacgg ccgtgagtgg agtcatattt cagcatgtat
     5281 gccatgtaat cagcgtctaa caggtcgttg attgcaacga tctcgatgtc agaacgtttc
     5341 tgagcagcac ggaaaacaat gcgaccgata cggccaaaac cgttgatacc tactttgata
     5401 gtcatatatt ccaccagcta tttgttagtg aataaaaggt tgcctgtaaa attacaaaaa
     5461 ccttacgcag cgtcaagcgg aatcgtgtca atcattgcga caaatcaatc ctgtgcctaa
     5521 gcattacgcg actgactcgc ctcacttttc ctttgggctt gagaccacat ggggtcggcg
     5581 ccccgaattt taaagggcaa ttacgataaa aatgtgattt tcatcacgat ttaggtgcag
     5641 ccatttcgtc gagatcaagt ttaggacaaa attttgccag ttggtgttaa tgttttgtta
     5701 gaatcagtca cgtaatgtga gcacgattaa agtgagatgt gagtaaatgg cgaataaacc
     5761 ttcggcagaa gaactgaaaa aaaatttgtc cgagatgcag ttttacgtga cgcagaatca
     5821 tgggacagaa ccgccattta cgggtcgttt actgcataac aagcgtgacg gcgtatatca
     5881 ctgtttgatc tgcgatgccc cgctgtttca ttccgaaacc aagtatgatt ccggctgtgg
     5941 ctggcccagt ttctacgaac cggtgagtga agaatccatt cgttatatca aagacttgtc
     6001 acatggaatg cagcgcatag aaattcgttg cggtaactgt gatgcccatc tggggcatgt
     6061 ctttcccgac gggccgcaac caacaggcga acgttattgt gttaactctg cttctttacg
     6121 cttcactgat ggcgaaaacg gcgaagaaat caacggttga aaaaacgatt cagcaactta
     6181 ttccacagga gcggaaaaat tatgaatctt gatgacatta tcaacagcat gacgcctgag
     6241 gtataccagc gtttgtcgac cgccgttgaa ctggggaaat ggcctgatgg cgttgcgttg
     6301 accgaggaac aaaaagagaa ttgcctgcaa ctggtgatgc tgtggcaagc ccgccataat
     6361 accgaagcac agcatatgac aattgacaca aatggtcaga tggtcatgaa gagcaaacag
     6421 cagttaaaag aagattttgg tatctcagca aagcctattg ccatgtttaa gtaagtggtt
     6481 catccccttt ttgccgcggc tatgaagggg atagccaaca atgaccccac gactcgtctc
     6541 aattatctcg acccgacctg gctaagtccc agtccgatct cttgcagtcg cgttcacgcc
     6601 tgaatgactt ctcctgaact taagtctcag acctatttgg ccggtaatcc ctctcgaatt
     6661 tgctcttttt gcgtcccgcc ttccaccaac ctgtaacaga cccagccatt ttctgcgtaa
     6721 ttgtgatttg agttgtaaaa tgcaggtcat tatctgtgtt gtgcagattt aatattgacc
     6781 tcaaaaatca tgtgggtaag tatgatggcc atctaagcaa aacaaaacac agaggatgac
     6841 gcaatgaaag cactggctcg gtttggcaag gcctttggcg gctacaagat gattcatgtc
     6901 ccacaaccca tttgtggccc ggaagacgta gtgactgaaa ttaaagccgc ggcaatctgc
     6961 ggcgcagaca tgaagcacta caatgtcgat agcggttctg atgagtttaa ctctatccgc
     7021 ggccatgagt tcgcaggttg tattgcgcag gttggtgaaa aagtcaaaga ctggaaagtg
     7081 gggcaacgcg tcgtgtcgga taacagcggt cacgtttgcg gcgtttgtcc ggcctgtgaa
     7141 cagggtgatt ttctgtgttg tacagaaaaa gtaaaccttg gtctggataa caatacctgg
     7201 ggcggtggtt tttccaaata ttgtctggtt cctggtgaaa ttctcaaaat tcatcgtcat
     7261 gcgttgtggg aaatccctga tggtgttgat tatgaggacg cagccgtact tgaccctatc
     7321 tgtaatgcct ataaatccat cgcgcagcaa tcgaaattcc ttcctggtca ggatgttgtc
     7381 gtcattggca ctggcccact cgggctgttt tccgtacaaa tggcgcggat tatgggggcg
     7441 gtaaatatcg tcgtcgttgg tctgcaagaa gatgtggcgg tccgcttccc ggttgcaaaa
     7501 gaactggggg cgacggcagt agtaaatggt tctaccgaag atgtggtggc gcgctgccag
     7561 caaatttgtg gcaaagataa tctgggactg gtgattgaat gctccggtgc caatatcgca
     7621 ctgaaacaag ccatcgaaat gctccgcccg aatggggaag tggtacgcgt tggaatgggc
     7681 ttcaaacctc ttgatttctc gattaatgac attaccgcct ggaataaaag catcattggg
     7741 catatggcct atgactccac ctcatggcgt aacgctatcc ggctattagc cagcggcgct
     7801 atcaaagtca aaccgatgat cacgcatcgt atcggcctgt cgcaatggcg cgaagggttt
     7861 gatgcgatgg tcgataaaac cgcaatcaaa gtgatcatga cttacgactt tgatgaataa
     7921 ctactctcct gaaggaacgt tatcgcatgg aacagataac aaaaccgcat tgtggtgcac
     7981 ggctggatcg cttaccggat tgccgctggc attcgtcaat gtttgccatc gtcgcgtttg
     8041 gcttgcttgt ctgctggagt aatgccgttg gtggcttgat cctcgcgcag ctgaaagcgt
     8101 tgggctggac agataattcc actacagcca cattctcagc aatcacgacc gccggaatgt
     8161 ttctcggtgc gctggtgggc ggcatcattg gcgacaaaac cggtcgcaga aatgcgttca
     8221 tcctctatga ggccattcat attgcctcga tggttgtcgg tgctttctca ccgaatatgg
     8281 attttcttat tgcctgccgt tttgtgatgg acgttgggct gggagcttta ctggttacac
     8341 tgtttgctgg tttcaccgaa tatatgcccg gtagaaatcg cggaacgtgg tccagtcggg
     8401 tttcctttat tggcaactgg tcatatccgc tctgctcact gattgcgatg ggactcacgc
     8461 cgctgattag tgcagagtgg aactggcggg tacaactgct tattcctgca atattgtcgc
     8521 ttatcgctac ggcgctggcc tggcgctact ttcctgaatc cccgcgctgg ctggaatcgc
     8581 gcggacggta tcaggaagcc gagaaagtca tgcggagtat agaagaaggc gtcatacgcc
     8641 agacgggtaa acctttgccg cctgtggtta ttgctgatga cggtaaagcg ccacaagcgg
     8701 tgccgtattc agccttactg acaggagtat tactgaaacg cgtgatatta ggctcttgtg
     8761 tgctgattac catgaacgtt gtgcagtaca cactaattaa ctggttgcca acaatattca
     8821 tgacccaggg gattaattta aaagactcga ttgttttaaa taccatgagt atgtttggcg
     8881 cgccatttgg tatttttatt gccatgctgg tgatggataa aattccgcgt aaaacaatgg
     8941 gtgtggggct attaatcctg attgcggtgc tcggatatat ctattcactg caaaccagta
     9001 tgttgctcat aacgctgatt ggtttcttcc tgattacttt cgtctatatg tacgtttgct
     9061 atgcctcggc agtgtatgtc cctgaaatct ggccgacaga ggccaaactc cgtggctccg
     9121 gtctggcgaa tgcggtaggg cgaatcagtg gtattgccgc accttatgcc gttgcagtgc
     9181 tgctcagtag ttatggtgta acgggagtct ttattcttct gggggctgtt tcaaatattg
     9241 tcgcaattgc tatcgccacc attggaattg aaaccaaagg tgtctccgtt gaaagtttaa
     9301 gtattgatgc agtagccaat aaataatacg ggtggattca aaatgaaaaa ttcaaaagca
     9361 atattgcagg tgccgggcac aatgaaaatt atttcagcag aaataccagt gcctaaagaa
     9421 gatgaagttt tgattaaagt agaatatgtc ggtatttgtg gttcagatgt acatggtttt
     9481 gaatcaggcc cgtttattcc gcctaaagac ccaaatcaag aaattggcct gggtcatgaa
     9541 tgcgccggga cggttgtggc tgtgggaagc cgcgtgcgca aatttaaacc gggggatcgg
     9601 gtaaatatcg aacctggcgt tccttgcggt cactgtcgtt actgtctgga aggcaaatat
     9661 aacatctgcc cggacgttga ttttatggcg acacaaccca actaccgcgg cgcattaacg
     9721 cactatctgt gtcatccgga gagctttact tacaaactgc ccgacaatat ggacacgatg
     9781 gaaggggcgc tggtggagcc tgccgcagtc gggatgcatg ccgcgatgct ggcagatgtt
     9841 aaaccgggta agaagataat tattctggga gcaggttgta ttggtttgat gacgttgcaa
     9901 gcgtgcaaat gcctgggcgc aacggaaatt gccgtcgttg atgtactgga aaaacgtctg
     9961 gcaatagggg aacagcctgg tgcgacagtg gttattaatg gcgcaaaaga ggacactatt
    10021 gcacgctgtc agcaatttac cgaagacatg ggcgcagata ttgttttcga aacagcgggt
    10081 tctgcggtca ccgttaaaca ggcaccttta atctggtaat gcgcggcggt aaaattatga
    10141 ttgttggtac tgtacccggc gattcggcaa tcaatttcct caaaatcaat cgcgaagtca
    10201 ctatccagac ggtattccgc tatgccaatc gttatccggt cacgattgaa gctatttctt
    10261 cagggcgatt cgatgtgaaa tcgatggtga cgcatattta cgattatcgg gatgtacaac
    10321 aggcatttga agagtcagtt aacaacaaac gcgacattat taaaggcgtt attaaaatta
    10381 gcgattaatt aatacgggag aacagaacat gctcgcagat atcagatatt gggaaaacga
    10441 tgccacaaat aaatattatg caattgccca ttttaacgta tggaatgcag aaatgttgat
    10501 gggcgttaaa gacgcagccg aagaagcgaa atccccggtt attatttctt ttagtacagg
    10561 ttttgtcggt aacacctcat ttgaagattt ctctcacatg atggtatcaa tggcacaaaa
    10621 agcaacggtg ccggtaataa ctcattggga tcatggtcgg agtattgaga ttattcataa
    10681 cgcctggact catggtatga attcattaat gcgtgatgct tctgcatttg atttcgaaga
    10741 aaatattcgt ttaaccaaag aggctgttga tttctttcat ccgctgggta ttccggtaga
    10801 ggcggaatta gggcatgtcg gtaatgaaac cgtttatgag gaagcgctgg cgggctatca
    10861 ctataccgat cctgaccagg cggctgaatt tgttgaacgt acgggctgtg actcattggc
    10921 tgtcgccatc ggcaaccagc atggggttta tacgtcagag ccgcaattga actttgaggt
    10981 cgtcaaacgc gtacgcgatg ctgtttctgt tccgctggtt ttgcatggcg catcggggat
    11041 cagtgatgcc gacattaaaa ctgcaatttc gttgggtatt gcgaaaatca acattcatac
    11101 ggagctctgt caggccgcaa tggtagcagt gaaagaaaac caggatcagc ctttcctgca
    11161 tttagaacgt gaagtacgta aagctgtaaa agaacgggca ttggaaaaaa ttgaactgtt
    11221 cggttctgat ggcaaagcgg aataatgata tggataatct cgacgttatt tgtataggtg
    11281 ccgctattgt tgatattcca ttacaaccgg tcagtaaaaa tatctttgat gtagattctt
    11341 atcctcttga aagaatcgca atgagcaccg ggggcgatgc aataaatgaa gcaacaatta
    11401 tttctcgtct ggggcatcgt accgcattaa tgagccgcat tggtaaagat gcctcagggc
    11461 aatttattct cgaccattgt cgcaaagaga atattgatat tcaaagcctg aaacaggacg
    11521 tcagcataga tacatctatt aacgttggac tggtgacgga ggatggcgag cggacgtttg
    11581 tcacgaaccg taacggcagt ttgtggaaat taaatattga cgatgttgat tttgcgcggt
    11641 ttcctcaggc gaaattatta tcactggcca gtattttcaa cagtcctcta ttggatggta
    11701 aagcgctaac agaaattttt acgcaagcga aagcccggca gatgatcatt tgtgccgata
    11761 tgatcaaacc gcggttgaat gaaacgctgg atgatatttg cgaagcattg agttatgtcg
    11821 attatctgtt tccaaatttt gccgaggaaa aattactcac agggaaagag acactggatg
    11881 aaattgctga ctgctttctt gcgtgcggcg taaaaacggt ggtgattaaa acgggtaaag
    11941 acggctgctt tatcaagcgt ggtgacatga cgatgaaggt gccggcggtc gcaggaataa
    12001 ccgccatcga caccattggc gcgggcgata actttgcttc aggttttatt gcggcactgt
    12061 tagaaggcaa aaatctgcgt gaatgcgcac gctttgccaa tgcaacggcg gctatctcgg
    12121 ttctaagcgt cggtgccacc accggcgtaa aaaacagaaa gctggtcgaa caattgctgg
    12181 aagaatacga aggataatga aggcaaatga aaaagatacc tttaggcacg acggatataa
    12241 cgctttcgcg aatggggttg gggacatggg ccattggcgg cggtcctgca tggaatggcg
    12301 atctcgatcg gcaaatatgt attgatacga ttcttgaagc ccatcgttgt ggcattaatc
    12361 tgattgatac tgcgctagga tataactttg gcaatagtga agttatcgtc ggtcaggcgt
    12421 taaaaaaact gccccgtgaa caggttgtag tagaaaccaa atgcggcatt gtctgggaac
    12481 gaaaaggaag tttattcaac aaagttggcg atcggcagtt gtataaaaac ctttccccgg
    12541 aatctatccg cgaagaggta gaagccagct tgcaacgtct gggtattgat tacatcgata
    12601 tctatatgac gcactggcag tcggtgccgc cattttttac gccgatagct gaaactgtcg
    12661 cagtgcttaa tgagttaaag gctgaaggga aaattcgcgc tataggcgct gctaacgtcg
    12721 atgctgacca tatccgcgag tatctgcaat atggtgaact ggatattatt caggcgaaat
    12781 acagtattct cgaccgggca atggaaaacg aactgctgcc gctatgtcgt gataatggca
    12841 ttgtggttca ggtttattcc ccgctagagc agggattgtt gaccggcact atcactcgtg
    12901 attacgttcc gggcggcgct cgggcaaata aagtctggtt ccagcgtgaa aacatgctga
    12961 aagtgattga tatgcttgaa cagtggcagc cactttgtgc tcgttatcag tgcacaattc
    13021 ccactctggc actggcgtgg atattaaaac agagtgattt aatctccatt cttagtgggg
    13081 ctactgcacc ggaacaggta cgcgaaaatg tcgcggcact gaatatcaac ttatcggatg
    13141 cagacgcaac attgatgagg gaaatggcag aggccctgga gcgttaaata ctcaacacga
    13201 ttcacctctg tttctgtgaa aactgtgatg aaaatctgtg attcaaatag gttattttga
    13261 aagtaaacat cggttatgcg ataatcgcgc taatgtgatg taaaaagata caggggtgta
    13321 atcgtggcgg caaaagacag gattcaggca attaagcaaa tggttgccaa cgataaaaag
    13381 gtgacagtct caaatttgag tgggattttt caggtaaccg aagaaaccat tcgccgcgat
    13441 cttgagaagc tggaagatga aggctttttg accagaacct atggtggtgc tgttttaaat
    13501 acagcggtgt tgacggagaa tatccatttt tataagcgcg cttcatcgtt ttatgaagag
    13561 aagctgctta ttgcacgcaa ggcactaccc tttatcgaca ataaaaccac catggcagcc
    13621 gattccagta gtaccgtaat ggaattgctc aaattgttac aggaccgtag tgacctgacg
    13681 ttgctaacca actcggcgga agcgattcat gttctggctc agtcagaaat taaagtcgtt
    13741 tcaacgggtg gggaactaaa caaaaataca ctttccctgc aaggaagaat tactaaagag
    13801 atcatcagcc gctaccatgt cgatatcatg gtaatgagtt gtaaaggtct tgatattaac
    13861 agcggcgcgc tggactctaa tgaagcggaa gctgaaatca aaaagacaat gatccgtcag
    13921 gcgacagaag ttgcgttatt ggttgatcac tctaagtttg atcgtaaagc ttttgtccag
    13981 ttagctgact ttagtcatat taattatata ataactgata aatcaccggg tgcagagtgg
    14041 atagcatttt gcaaagacaa taatatccag ctggtgtggt aatttaagca cacgttgtca
    14101 atattatttg ttcctgcgac tggaaataaa atgatgataa agatgcggca agtgaatttg
    14161 ccgcaattgc taccttttat agcaaatccg aggcacccaa tggaacaata tgatcaaatt
    14221 ggcgcaagac tggaccgctt gcctttggcc cggtttcatt atcgtatatt tggtataata
    14281 agctttagtc tgttattaac ggggtttttg agttactcag gtaatgtcgt cttagcaaag
    14341 ctggtaagca atggatggtc aaataatttc ctctatgccg cctttacctc ggcattaatg
    14401 tttggttatt tcatcggctc acttactggt gggtttattg gtgactactt tgggcggcgc
    14461 agggcgtttc gcataaatct tctcatcgtc ggtattgctg caacaggggc cgcttttgtc
    14521 cctgatatgt actggctcat tttctttcgc ttcctgatgg gaacaggaat gggggcgctg
    14581 attatggttg gctatgcctc atttacggag tttatccccg cgacggtgcg tggaaaatgg
    14641 tccgcgcggc tctcatttgt tggtaactgg tcgcccatgc tgtctgcggc gataggcgtg
    14701 gtggttatcg ctttttttag ttggcgaata atgtttcttc tgggtggtat tggcatactg
    14761 ttagcctggt ttctctcagg taaatacttt atcgagtcgc cacgatggct ggaagggaaa
    14821 gggcaaatcg ccggtgcaga aagccaactt cgtgaagtag agcagcaaat tgaaagggag
    14881 aagagtattc gtttacccca gcttactttg aaccagagca acagcaaggt taaggtaatc
    14941 aagggtacct tcaggctcct gtttaaaggg gaaatgttac gacgtacatt agttgcgata
    15001 actgttttaa ttgcaatgaa catttcgctt tataccatta ccgtatggat accgaccata
    15061 tttgttaact ccggcattga tgtcgataaa tcaatattaa tgaccgctgt tattatgatt
    15121 ggtgctccgg taggaatatt tattgcggca ttaattattg atcattttcc tcgtcgatta
    15181 tttggctccg ccttacttat tattattgcc gtgttaggct atatctattc aagtcagact
    15241 acagagtggg cgattttaat ctatggtctg gtgatgatct tctttttata catgtatgtt
    15301 tgcttcgcgt cggcggttta tatcccggag ctttggccaa cgcatttacg cctgcgcggt
    15361 tcgggtttcg ttaatgccgt cggacggatc gtcgcagttt tcacgcccta tggcgttgcg
    15421 gcattattaa cacattatgg gtcgatcacg gtgtttatgg tgcttggtgt catgttagtg
    15481 ctctgtgcgc tggttctctc catttttggc atcgaaacgc ggaaggtgtc gctggaagag
    15541 atttctgagg tgaattaaat ctttcgttga tggcatcaat tgccagatgt accactgacg
    15601 tgttttattt ggcctacaat gccagtacag aattgtaggc cagcgtaaaa ttacccctgc
    15661 gtctcttccc agtctgccag cgtatatagc gttgccccag ctgctgacat ctccataaac
    15721 gcgtgtgcac tgtcctgggg ctggatattc acgccacgac agccatcggt aatcacgttt
    15781 accttataac ctaactgtaa cgcgtccagc acggtaaact tcacgcaata gtcagtagcc
    15841 aggcccataa cgatcaattc atcgatttca tgatcgcgca accagtcatc aagcgcggtt
    15901 ttctgccgac gtccgttatc aaaaaaggca ctgtaactgt caactaaagg attttcgcct
    15961 ttatggaaca ccgctgcgat ctctttttga ttcagtaacg gatgtaattg tgcgccttca
    16021 ctgttctgca cacagtgttc aggccagaaa gtttgccgca ggccgccgag ttggcctggc
    16081 gtataaggct ctacgccgtg ctggctggca aagctgccgt gattcgccgg atgccagtcc
    16141 tggctggcga taaccgcttc tccgcgcgac tggcaccagt caataaggcg gttagcaacc
    16201 tccaccgtac tgtcaccttc cggtacggcg agcgcgccac cagcacagaa atcattttgt
    16261 aaatcgacca gtaacagggc gcgatggggc attgcagtct ccttaatcat ccggcgtcag
    16321 ttcgccgcgc aggttttggc tcatggcctt gcgaatggtt tcagtatcca gttcctggct
    16381 cagcaggtaa tgcagtttgg ttagcgtggc ttctacagtc atatctgcac cgtcaataac
    16441 gccggcatgg gcgagggcgt taccggtggc ataaccactc atattcactt taccggacat
    16501 acattgtgtc aggttgacca ccacaatacc gcgatcgctg gcttcttgta attcctgcag
    16561 gaaggctttg ttttgtggcg cattacccac gccataggag cgcagaatca atgctttcac
    16621 cggttggcgc agaaaattgc gcacgacgtc agcagaaatc cctggataaa tcgtcaccac
    16681 gccaattggt tgtggcgtga ttggatgaac gattaattcc ccttcaccgt gcggggcggg
    16741 tggcgtattc aaacgacgaa tatggatacc agcttccagt aacggaggaa ggtttggaga
    16801 ggcaaacgca tcaaaaccat cggcatgggc tttggtagtg cggttgccgc gatacaatcg
    16861 gttattgaaa aagagcgtta cttcgttgat cggataattc ggcgcaacgt acaacgcatt
    16921 cagcagatta atttgtccgt cagagcgtaa ctcagccagc gggatttgtg accctgtcac
    16981 aatgaccggt ttaccgagat tctcgagcat gaacgacagc gcagaggcgg tatacgccat
    17041 cgtgtcggta ccgtgtagaa tgacaaaacc atcatagtcg tcatagtgcg ctttaatatc
    17101 ttcagcaata tgctgccagt cttccggcgt catatcagaa gaatccatca acggcgtata
    17161 ttcatgaatg gtgaaatctg gcatctccgg gcgatggaat tccggcatca gcgccagttg
    17221 gcgttgtaga tgacctgaca ccggaatata accctgctcg gaacgctgca tcccgatggt
    17281 cccgcccgtg taggcgacgt aaattgattt cttttgcatg atattgatat gaatgatatc
    17341 gaaagagcaa aagtatatac gtgaggcagg aggagcgcac ctgatactat gcggataagg
    17401 aaatatgggg cgagacagcg ggaagaggat tcgtcgaggt aaaaaataat gtggccgacg
    17461 atctgtcggc cactcagtac aagacttaac gcacgttggc gcaggtcagg caaaacgcat
    17521 aacggttttg tgggtcgtta aacgcggcca gcttgtcgct ttcactttta acggtagagg
    17581 ctaccgaggc aagcggtgca ggtaacatgg cctggaacgc atctggcaac attgcccgga
    17641 cagaaccaga catgttgtcc atcactttgt cgaagaaggt cggttcatca acgtagtatt
    17701 ccagatgcca ctgtttcact tttgcgagct ctgctgcttt ggcaaccgca tcatcgaaat
    17761 ccccgagact atcgaccagc ccgttagctt ttgcatcctg accggtccag acgtggccct
    17821 gggcgatttt atcaatttgc tccggcgtcg aatgacgcgc atcagcaacc agcgtgataa
    17881 agcgtttata gccattctca atgcttaatt gcatcatctg ctgcgcttcc ggcggcagtg
    17941 ccctggtgat agaaacatcc gccagcggtg aagttgagac accatcagta tgaacaccaa
    18001 tcgaatccag actattttct acggtggtga tcacgccgaa gataccgata gaaccggtca
    18061 gggtgctggg gttagccaca atgtaattag ctggcgtgga aatccagtaa ccaccagatg
    18121 ccgccatgcc gcccatcgat acaaccacag acttacccgc tgcccgggct gctgccagtt
    18181 cagcgcgaat cacttcagac gcggtaacgc tgccgcctgg gctattaaca cgcaggacaa
    18241 tcgctttcac tttcgggtca aggcgagcgt cgcggatttg tgccgcagtg gtatcaccgc
    18301 caacattccc ctgagtttcc tcgccatcca taattgcgcc attagcgaag acgacaccga
    18361 tgctgtcacc ggtatctgcc ggcgttttca atgcgtaatc gtaataactg atggcgcgat
    18421 aatttttatc agtcttactc cagccgaact ctttggtcag agctttttcg atttcggcac
    18481 tcgatgccag tgcatcgacc agcttgtttt ccagtgcata tttcgcggta tcgccaccgg
    18541 ttttggttaa accctcaagc aacccttgcg cgccagggaa tacctgctga gcagggatct
    18601 gccggttagc ggcaacagta ttcagatagt tttgccacag ctcaccaatc cagcggctgt
    18661 cagcttcgcg ggctgccggt gacatatcat cacgaataaa cggttcaacg gcagatttat
    18721 acgtacccac gcggaacaca tgggtggaaa ctttcagctt atccagcaac gatttgtagt
    18781 acagaccgtt ggtagcaaaa ccgtgcagat caaccacgcc ttgcggtgac agccaaattt
    18841 tattggcgaa actggcgaga taatattgcc cctggctgta gttctcgcca acggcataaa
    18901 ccggtttccc gctgtcacga aactctttca gggctttgcc gatgtactgc atagacggct
    18961 ggtcgccgcc tgcgaagttt ttcagatcca tcacgatacc ggtgatattg cggtcgtcct
    19021 tcgcctggcg aatggtgttg acgatatcaa acagtgagtt ttcctgcaga cgatcggaac
    19081 tggcaccaag cagctggcgg cttaatttac taaaccgctg agaactgtct ggtttatcga
    19141 cgatcacacc agaaatgtcc agcagcagtg cgccacgact ggccgtttct ttcgaatcac
    19201 caccactgac ctgcatccag atccccacac caaccagtac gaggaaaata aagaacaggt
    19261 taagtaccat ttcacggacg aaattcagca gacgccacgt ccatttaaaa aatccggcaa
    19321 taaatcgcca aagggttcgc atgtattctc ccaacttaag gtcacacctg tcacaggcgc
    19381 aatagatgtg gttatcgtaa tgacccaacc ggcacttgtc agcaggaatt gcggactgtg
    19441 ctgtaacaaa atccactctc gtgttaattt tgtgaataaa tatcacgaca ggagttaatc
    19501 aaatggatgc actcgaacta ttgatcaatc gccgtagcgc ctcccgcctg gctgaacccg
    19561 cgccaacggg tgaacaactg caaaacatcc tgcgtgcggg tatgcgtgcg ccggaccata
    19621 agtccatgca accgtggcat ttttttgtga ttgaagggga agggtgcgag cgtttcagcg
    19681 ccgtactgga acagggggcg attgctgccg gtagtgatga taaagctatc gacaaagccc
    19741 gtaatgcgcc gttccgcgca ccgctcatca tcacggtggt ggcgaaatgc aaagaaaatc
    19801 ataaagtccc gcgatgggaa caggaaatgt ctgccggatg cgcggtcatg gcgatgcaaa
    19861 tggcagccgt tgcccaggga tttggcggca tctggcgcag tggcgcatta actgaaagtc
    19921 cggtagtgcg tgaagcattc ggttgccgtg agcaggataa aattgtcggt tttctctacc
    19981 tcggcacgcc gcagttgaaa gcatctacgt cgattaacgt cccggacccg acgccgtttg
    20041 taacttattt ctgataattg tcgcgaaact gtctggattg tgagcaaagg cgcaggaatt
    20101 cagacactct cacttatcac ttcacggaat gagggttacc atacccggat tgcattgaca
    20161 ggagatgtcc atgagcgaga actcgattcg tttgacccaa tacagccacg gagctggttg
    20221 cggctgtaaa atttccccaa aagtgttgga aaccatcctg cacagtgagc aggcgaagtt
    20281 tgttgatccg aatttgcttg tgggtaatga aacccgcgac gatgcggcgg tgtacgatct
    20341 gggcaatggc accagcgtta tcagtaccac cgacttcttt atgccgatcg ttgataatcc
    20401 tttcgatttt ggccgcattg cggcgactaa cgccatcagc gatatcttcg cgatgggggg
    20461 caaaccgatt atggcgattg cgatcctcgg ctggccgatt aacaaacttt ccccggaaat
    20521 tgcccgcgaa gtaaccgaag gtggacgcta tgcatgccgt caggcgggta ttgcgctggc
    20581 tggcggtcac tccatcgatg cgccggagcc gatttttggt ctggcggtaa cggggatcgt
    20641 accgaccgag cgggtgaaga aaaacagtac cgcacaagcc ggatgcaaac tgttcctgac
    20701 gaaaccgctg gggatcggcg ttcttaccac ggctgagaaa aaatcactgt tgaaaccaga
    20761 acatcaggga ctggcgacgg aagtgatgtg ccggatgaac atcgcaggcg cgtcctttgc
    20821 caacatcgaa ggcgtaaaag cgatgaccga cgttacgggc tttggtctgc tgggccactt
    20881 gagcgaaatg tgtcaggggg ctggtgtgca ggcacgcgtc gactatgaag cgatcccgaa
    20941 actccccggt gttgaagagt acattaagtt gggcgcagta cctggcggca ctgaacgtaa
    21001 ctttgccagc tacggtcatc tgatgggtga aatgccgcgt gaagtgcgcg atctgctgtg
    21061 cgatccgcaa acttctggcg gtttgctgct ggcggtcatg ccggaagcag aaaatgaggt
    21121 taaaactaca gccgccgagt ttggcattga actgacggcg attggcgaac tggtgccagc
    21181 gcgcggcggt cgtgccatgg ttgagatccg ttaatttaat gcggttgttt attgccgaaa
    21241 aaccgagtct ggcgcgcgcc attgctgatg tcctgcccaa accgcaccgg aaaggcgatg
    21301 gctttatcga gtgcggtaat ggtcaggtgg tgacttggtg tatcggtcac ctgcttgagc
    21361 aggcgcagcc agacgcctac gacagccgct atgcgcgctg gaatcttgcg gatttgccga
    21421 ttgtcccgga aaagtggcaa ttacagccac gaccctccgt gaccaaacaa cttaacgtca
    21481 tcaaacgctt cctgcatgaa gccagcgaaa tcgttcacgc cggggacccg gatcgtgaag
    21541 ggcaattgct ggtggatgaa gtgctggact atctgcaact ggcaccggaa aagcgccagc
    21601 aggtgcagcg ttgcttgata aacgacctga acccgcaggc ggttgagcgg gcgatcgacc
    21661 gtcttcgttc caacagtgag tttgtgccat tgtgcgtttc tgcgctggcg cgagcgcgtg
    21721 ccgactggct gtacggcatc aatatgaccc gtgcgtatac cattctcggt cgcaatgccg
    21781 gttatcaggg cgtactttcc gtgggacgcg tgcagacacc cgtgctcggg ctggtggtgc
    21841 gccgcgatga agagattgaa aacttcgtgg cgaaagactt ctttgaagtc aaagcacata
    21901 tcgtgacacc tgccgatgag cggtttaccg ctatctggca accgagcgaa gcgtgtgaac
    21961 cgtaccagga tgaagaaggg cgcttgttac atcgtccact ggcggagcat gtggttaacc
    22021 gcattagtgg tcaaccggct attgtcacca gctataacga taaacgggaa tcagaatccg
    22081 cgccgctgcc gttttcgctt tcggcgttgc agattgaagc ggcaaaacgc ttcggtctga
    22141 gcgcgcagaa cgtgcttgat atctgccaga agctgtacga aacgcacaag ctaatcactt
    22201 atccgcgttc cgattgtcgc tatttgccag aagaacattt tgccggacgc cacgcggtga
    22261 tgaatgccat cagcgttcat gcaccggatc tgttgccgca gccagtggta gatccagata
    22321 tacgcaaccg ctgttgggat gacaaaaagg tcgatgcgca ccacgccatc atcccgaccg
    22381 cacggagttc tgcgatcaac ctgacggaga acgaagcgaa ggtctataac ctgattgccc
    22441 gtcaatacct gatgcagttc tgcccggatg cggtgttccg caagtgtgtt atcgaactgg
    22501 acattgccaa aggcaaattt gtcgctaaag cccgttttct tgctgaagca ggctggcgca
    22561 cgctgttagg cagcaaagag cgcgatgaag aaaacgacgg cacgccattg cctgtggtgg
    22621 cgaaaggcga tgagttgctg tgtgaaaaag gtgaagtggt agagcggcaa acccagccgc
    22681 cgcgccattt taccgatgca acactgcttt cggcgatgac cgggatcgcg cgctttgtgc
    22741 aggacaaaga tctgaaaaag gtccttcgtg cgaccgatgg tctggggaca gaagcaaccc
    22801 gtgccgggat tattgaactg ctgttcaagc gtggttttct gaccaaaaaa gggcgctata
    22861 tccactccac cgacgccgga aaagcgttat tccattcgct gccagaaatg gcgacgcgac
    22921 cggacatgac cgcgcactgg gaatcggtgc tgacgcaaat cagcgaaaag cagtgtcgct
    22981 atcaggactt tatgcagccg ctggtgggga cgctatatca gcttattgat caagccaaac
    23041 gtacgccggt gcggcagttt cgcggcattg tggctccggg cagtggtggc agtgctgata
    23101 agaaaaaggc tgcaccgcgt aaacgtagtg cgaaaaaaag tccgccagca gatgaagccg
    23161 gaagcggggc gatagcgtaa gcgagcgaat ctttcgtgct attcgagtca tattctgaaa
    23221 tatccagcgg atcaagaaag ttcgttggat atttttttgc atggataaaa ttatcgcctc
    23281 taaagtatgt aataacaggg aatgtgatga aaaaagtttt attgcaaaac catcctggga
    23341 gcgagaagta ttcttttaat ggctggggaa tatttaatag taattttgaa cgggtgatta
    23401 aagaaaataa ggccatgctg ctttgtaagt gggggtttta tttaacatgt gttgtcgctg
    23461 taatgtttgt attcgcagcg ataacatcca acggtttgaa tgaaagaggc ctgattaccg
    23521 cgggatgctc ttttctttat ctattaatta tgatggggct tattgttcgg gccggtttta
    23581 aagcaaaaaa agaacaactg cattattatc aggctaaagg tattgagccg ctcagtatcg
    23641 agaagttaca ggcgctacaa ttgatcgcac cttatcgatt ctatcataag caatggtctg
    23701 aaacgctaga gttctggccg cgaaagcctg aacctggcaa agataccttc caatatcatg
    23761 tgcttccttt tgactcgatc gatatcatca gtaaaagacg cgagtcttta gagaatcaat
    23821 ggggtatcga agatagcgaa agttattgtg ccttaatgga gcattttctt tctggcgacc
    23881 atggagccaa tacctttaaa gcaaacatgg aggaagcccc agagcaggtt atcgccttgt
    23941 tgaataaatt tgctgttttt ccctcagact atatctctga ttgcgctaat aatcgctccg
    24001 gtaaatcctc ggcgaagcta atatgggcgg cggaattatc atggatgatc tcgatatcaa
    24061 gcacagcttt tcaaaacggg acaattgaag aagaactggc ctggcattat ataatgcttg
    24121 cttctcgaaa ggcgcacgag ttgttcgaaa gcgaagaaga ttatcaaaaa aatagtctaa
    24181 tgggatttct ttactggcat atctgctgct atcgcagaaa gttaacggat gctgaacttg
    24241 aagcgtgtta tcgttacgac aagcagtttt gggagcacta cagtaagaaa tgccgttggc
    24301 ccataagaaa tgttccctgg ggagcatcat ccgttaaata ctcataaacg cctgaaattt
    24361 tgccgggggc gctgcgcttg accggcctac attagagcgt aactgcaatg aattgtgccc
    24421 atctgtaggc ctgataagcg tagcgccatc aggcatttac aacttaaatc acaccctgcg
    24481 ccagcatcgc atcggcaacc ttcacaaaac cggcaatgtt cgcgccctgc acgtagttgg
    24541 tttgctcacc ttcaccacca tgctcaacac aggcatggtg gatatccagc atgatgtgat
    24601 gcaaacgtgc gtcaactttc tcggctttcc agcccaggcg cgcagcgttt tgtgccattt
    24661 ccaggcccga cgttgctaca ccgccagcat tagccgcttt acccggtgca aacagtacgc
    24721 ctgcctgctg gaacagttca gtcgcttcga tggtggtcgg catatatgcc ccttcagcga
    24781 cggctttaac gccattggcg ataagctgat gcgcggcgtc aacatccagt tcgttctggg
    24841 tggcgcaagg cagggcgata tcaaccggta cagaccacgg ctgttggcct tcgagataga
    24901 ccagaccaaa ttctttggcg taatctgccg ctcgaccatc gcggctggct ttgatttcga
    24961 taagacgtgc cagtttctct ttcgtgaatc cgctttcatc aactacagtg ccgctggagt
    25021 ctgacgcagt gatcacacga gcaccaaatt ccatcgcttt ttcgatagcg tactgggcga
    25081 cgttgccgga gccagaaacg gaaacgctca tcccttcaaa acccataccg tggcgtttta
    25141 aca
//
LOCUS       DAJTRC010000057        24229 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.057, whole genome shotgun sequence.
ACCESSION   DAJTRC010000057 DAJTRC010000000
VERSION     DAJTRC010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24229
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.057"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            484..2412
                     /gene="thrS"
                     /locus_tag="OO357_002469"
     CDS             484..2412
                     /gene="thrS"
                     /locus_tag="OO357_002469"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006894721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HCR5887382.1"
                     /translation="MPVITLPDGSQRHYDHAVSPMDVALDIGPGLAKACIAGRVNGEL
                     VDACDLIENDAQLSIITAKDEEGLEIIRHSCAHLLGHAIKQLWPHTKMAIGPVIDNGF
                     YYDVDLDRTLTQEDVEALEKRMHELAEKNYDVIKKKVSWHEARETFANRGESYKVSIL
                     DENIAHDDKPGLYFHEEYVDMCRGPHVPNMRFCHHFKLMKTAGAYWRGDSNNKMLQRI
                     YGTAWADKKALNAYLQRLEEAAKRDHRKIGKQLDLYHMQEEAPGMVFWHNDGWTIFRE
                     LEVFVRSKLKEYQYQEVKGPFMMDRVLWEKTGHWDNYKDAMFTTSSENREYCIKPMNC
                     PGHVQIFNQGLKSYRDLPLRMAEFGSCHRNEPSGSLHGLMRVRGFTQDDAHIFCTEEQ
                     IRDEVNGCIRLVYDMYSTFGFEKIVVKLSTRPEKRIGSDEMWDRAEADLAVALEENNI
                     PFEYQLGEGAFYGPKIEFTLYDCLDRAWQCGTVQLDFSLPSRLSASYVGEDNERKVPV
                     MIHRAILGSMERFIGILTEEFAGFFPTWLAPVQVVIMNITDSQSEYVNELTQKLSNAG
                     IRVKADLRNEKIGFKIREHTLRRVPYMLVCGDKEVESGKVAVRTRRGKDLGSMDVNEV
                     IEKLQQEIRSRSLKQLEE"
     gene            2416..2958
                     /gene="infC"
                     /locus_tag="OO357_002470"
     CDS             2416..2958
                     /gene="infC"
                     /locus_tag="OO357_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HCR5887383.1"
                     /translation="MKGGKRVQTARPNRINGEIRAQEVRLTGLEGEQLGIVSLREALE
                     KAEEAGVDLVEISPNAEPPVCRIMDYGKFLYEKSKSSKEQKKKQKVIQVKEIKFRPST
                     DEGDYQVKLRSLIRFLEEGDKAKITLRFRGREMAHQQIGMEVLNRVKDDLQELAVVES
                     FPTKIEGRQMIMVLAPKKKQ"
     gene            3055..3252
                     /gene="rpmI"
                     /locus_tag="OO357_002471"
     CDS             3055..3252
                     /gene="rpmI"
                     /locus_tag="OO357_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HCR5887384.1"
                     /translation="MPKIKTVRGAAKRFKKTGKGGFKHKHANLRHILTKKATKRKRHL
                     RPKAMVSKGDLGLVIACLPYA"
     gene            3305..3661
                     /gene="rplT"
                     /locus_tag="OO357_002472"
     CDS             3305..3661
                     /gene="rplT"
                     /locus_tag="OO357_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HCR5887385.1"
                     /translation="MARVKRGVIARARHKKILKQAKGYYGARSRVYRVAFQAVIKAGQ
                     YAYRDRRQRKRQFRQLWIARINAAARQNGISYSKFINGLKKASVEIDRKILADIAVFD
                     KVAFTTLVEKAKAALA"
     misc_feature    3778..3902
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            3784..3828
                     /gene="pheM"
                     /locus_tag="OO357_002473"
     CDS             3784..3828
                     /gene="pheM"
                     /locus_tag="OO357_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pheST operon leader peptide PheM"
                     /protein_id="HCR5887386.1"
                     /translation="MNAAIFRFFFYFST"
     gene            4111..5094
                     /gene="pheS"
                     /locus_tag="OO357_002474"
     CDS             4111..5094
                     /gene="pheS"
                     /locus_tag="OO357_002474"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004851795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HCR5887387.1"
                     /translation="MSHLAELVASAKAAISQASDVAALDNVRVEYLGKKGHLTLQMTT
                     LRELPPEERPAAGAVINEAKEQVQQALNARKAELESAALNARLAAETIDVSLPGRRIE
                     NGGLHPVTRTIDRIESFFGELGFTVATGPEIEDDYHNFDALNIPGHHPARADHDTFWF
                     DATRLLRTQTSGVQIRTMKAQQPPIRIIAPGRVYRNDYDQTHTPMFHQMEGLIVDTNI
                     SFTNLKGTLHDFLRNFFEEDLQIRFRPSYFPFTEPSAEVDVMGKNGKWLEVLGCGMVH
                     PNVLRNVSIDPEVYSGFAFGMGMERLTMLRYGVTDLRSFFENDLRFLKQFK"
     gene            5109..7496
                     /gene="pheT"
                     /locus_tag="OO357_002475"
     CDS             5109..7496
                     /gene="pheT"
                     /locus_tag="OO357_002475"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HCR5887388.1"
                     /translation="MKFSELWLREWVNPAIDSDTLANQITMAGLEVDGVEPVAGSFHG
                     VVVGEVVECAQHPNADKLRVTKVNVGGDRLLDIVCGAPNCRQGLRVAVATIGAVLPGD
                     FKIKAAKLRGEPSEGMLCSFSELGISDDHSGIIELPADAPIGTDIREYLKLDDNTIEI
                     SVTPNRADCLGIIGVARDVAVLNQLPLVEPEIVPVGATIDDTLPITVEAPEACPRYLG
                     RVVKGINVKAPTPLWMKEKLRRCGIRSIDAVVDVTNYVLLELGQPMHAFDKDRIEGGI
                     VVRMAKEGETLVLLDGTEAKLNADTLVIADHNKALAMGGIFGGEHSGVNDETQNVLLE
                     CAFFSPLSITGRARRHGLHTDASHRYERGVDPVLQHKAMERATRLLIDICGGEAGPVI
                     DITNEATLPKRATITLRRSKLDRLIGHHIADEQVTDILRRLGCEVTEGKDEWQAVAPS
                     WRFDMEIEEDLVEEVARVYGYNNIPDEPVQASLIMGTHREADLSLKRVKTLLNDKGYQ
                     EVITYSFVDPKVQQMIHPGVEALLLASPISVEMSAMRLSLWTGLLATVVYNQNRQQNR
                     VRIFESGLRFVPDTQAPLGIRQDLMLAGVICGNRYEEHWNLAKETVDFYDLKGDLESV
                     LDLTGKLNEVEFRAEANPALHPGQSAAIYLKGERIGFVGVVHPELERKLDLNGRTLVF
                     ELEWNKLADRVVPQAREISRFPANRRDIAVVVAENVPAADILSECKKVGVNQVVGVNL
                     FDVYRGKGVAEGYKSLAISLILQDTSRTLEEEEIAATVAKCVEALKERFQASLRD"
     gene            7501..7800
                     /gene="ihfA"
                     /locus_tag="OO357_002476"
     CDS             7501..7800
                     /gene="ihfA"
                     /locus_tag="OO357_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit alpha"
                     /protein_id="HCR5887389.1"
                     /translation="MALTKAEMSEYLFDKLGLSKRDAKELVELFFEEIRRALENGEQV
                     KLSGFGNFDLRDKNQRPGRNPKTGEDIPITARRVVTFRPGQKLKSRVENASPKDE"
     gene            7901..8881
                     /gene="btuC"
                     /locus_tag="OO357_002477"
     CDS             7901..8881
                     /gene="btuC"
                     /locus_tag="OO357_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter permease BtuC"
                     /protein_id="HCR5887390.1"
                     /translation="MLTLARQQQRQNIRWLLCLSVLMLLALLLSLCAGEQWISPGDWF
                     TPRGELFVWQIRLPRTLAVLLVGAALAISGAVMQALFENPLAEPGLLGVSNGAGVGLI
                     AAVLLGQGQLPNWALGLCAIAGALIITLILLRFARRHLSTSRLLLAGVALGIICSALM
                     TWAIYFSTSVDLRQLMYWMMGGFGGVDWRQSWLMLALIPVLLWICCQSRPMNMLALGE
                     ISARQLGLPMWFWRNVLVAATGWMVGVSVALAGAIGFIGLVIPHILRLCGLTDHRVLL
                     PGCALAGASALLLADIVARLALAAAELPIGVVTATLGAPVFIWLLLKAGR"
     gene            8944..9495
                     /gene="btuE"
                     /locus_tag="OO357_002478"
     CDS             8944..9495
                     /gene="btuE"
                     /locus_tag="OO357_002478"
                     /EC_number="1.11.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional thioredoxin/glutathione peroxidase"
                     /protein_id="HCR5887391.1"
                     /translation="MQDSILTTVVKDIDGEVTTLEKYAGNVLLIVNVASKCGLTPQYE
                     QLENIQKAWADRGFVVLGFPCNQFLEQEPGSDEEIKTYCTTTWGVTFPMFSKIEVNGE
                     GRHPLYQKLIAAAPTTVAPEESGFYARMVSKGRAPLYPDDILWNFEKFLVGRDGKVIQ
                     RFSPDMTPEDPIVMESIKLALAK"
     gene            9495..10244
                     /gene="btuD"
                     /locus_tag="OO357_002479"
     CDS             9495..10244
                     /gene="btuD"
                     /locus_tag="OO357_002479"
                     /EC_number="7.6.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 ABC transporter ATP-binding protein
                     BtuD"
                     /protein_id="HCR5887392.1"
                     /translation="MSIVMQLQDVAESTRLGPLSGEVRAGEILHLVGPNGAGKSTLLA
                     RMAGMTSGKGSIQFAGQPLEAWSATKLALHRAYLSQQQTPPFAMPVWHYLTLHQHDKT
                     RTELLNDVAGALALDDKLGRSTNQLSGGEWQRVRLAAVVLQITPQANPAGQLLLLDEP
                     MNSLDVAQQSALDKILSALCQQGLAIVMSSHDLNHTLRHAHRAWLLKGGKMLASGRRE
                     EVLTPPNLAQAYGMNFRRLDIEGHRMLISTI"
     gene            10322..10786
                     /locus_tag="OO357_002480"
     CDS             10322..10786
                     /locus_tag="OO357_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5887393.1"
                     /translation="MRFCLILITALFLAGCSHHKAPPPNARLSDSITVIAGLNDQLQS
                     WHGTPYRYGGMTRRGVDCSGFVVVTMRDRFDLHLPRETKQQASIGTQIDKDELLPGDL
                     VFFKTGSGQNGLHVGIYDTNNQFIHASTSKGVMRSSLDNVYWQKNFWQARRI"
     gene            11033..11746
                     /gene="ydiV"
                     /locus_tag="OO357_002481"
     CDS             11033..11746
                     /gene="ydiV"
                     /locus_tag="OO357_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-FlhDC factor"
                     /protein_id="HCR5887394.1"
                     /translation="MKIFLENLYHSDCYFLPIRDNQQLLVGVELITHFSSEDGTVRIP
                     TSRVIAQLTEEQHWQLFSEQLELLKSCQHFFIQHKLFAWLNLTPQVATLLLDRDNYAG
                     ELLKYPFIELLINENYPHLNEGKDNRDLLSLSQMYPLVLGNLGAGNSTMKAVFDGLFT
                     RVMLEKGFIQQQITHRSFEPFIRAIQAQISPCCNCIIAGGIDTPEILAQITPFDFHAL
                     QGCLWPAVPINQITTLVQR"
     gene            11810..13246
                     /locus_tag="OO357_002482"
     CDS             11810..13246
                     /locus_tag="OO357_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiU family protein"
                     /protein_id="HCR5887395.1"
                     /translation="MTLSFITRWRDELPETYTALSPTPLNNARLIWHNTELANTLSIP
                     SSLFKNAAGVWGGETLLPGMSPLAQVYSGHQFVVWAGQLGDGRGILLGEQLLADGTTM
                     DWHLKGAGLTPYSRMGDGRAVLRSTIRESLASEAMHYLGIPTTRALSIVTSDSPVYRE
                     TVEPGAMLMRVAPSHLRFGHFEHFYYRREPEKVRQLADFAIRHYWSHLEDDEDKYRLW
                     FNDVVARTASLIAQWQTVGFAHGVMNTDNMSLLGLTLDYGPFGFLDDYEPGFICNHSD
                     HQGRYSFDNQPAVALWNLQRLAQTLSPFVAVDALNEALDSYQQVLLTHYGQRMRQKLG
                     FMTEQKEDNALLNELFSLMARERSDYTRTFRMLSLTEQHSAASPLREEFIDRAAFDDW
                     FARYRGRLQQDEVSDSERQQLMQSVNPALVLRNWLAQRAIEAAEKGDMMELHRLHEAL
                     RNPFSDRDDDYVSRPPDWGKRLEVSCSS"
     gene            complement(13250..13441)
                     /gene="hemP"
                     /locus_tag="OO357_002483"
     CDS             complement(13250..13441)
                     /gene="hemP"
                     /locus_tag="OO357_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemin uptake protein HemP"
                     /protein_id="HCR5887396.1"
                     /translation="MRYTDSRKLTPETDANHKTASPQPIRRITSQTLLGPDGKLIIDH
                     DGQEYLLRKTQAGKLLLTK"
     gene            complement(13573..14619)
                     /gene="aroH"
                     /locus_tag="OO357_002484"
     CDS             complement(13573..14619)
                     /gene="aroH"
                     /locus_tag="OO357_002484"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroH"
                     /protein_id="HCR5887397.1"
                     /translation="MNRTDELRTARIESLVTPAELALRYPVTPGVATHVTDSRRRIEK
                     ILNGEDKRLLVIIGPCSIHDLTAAMEYATRLQSLRNQYQSRLEIVMRTYFEKPRTVVG
                     WKGLISDPDLNGSYRVNHGLELARKLLLQVNELGVPTATEFLDMVTGQFIADLISWGA
                     IGARTTESQIHREMASALSCPVGFKNGTDGNTRIAVDAIRAARASHMFLSPDKNGQMT
                     IYQTSGNPYGHIIMRGGKKPNYHADDIAAACDTLHEFDLPEHLVVDFSHGNCQKQHRR
                     QLEVCEDICQQIRNGSTEIAGIMAESFLREGTQKIVGGQPLTYGQSITDPCLGWEDTE
                     RLVEKLAFAVDTRF"
     gene            complement(14776..15609)
                     /gene="ppsR"
                     /locus_tag="OO357_002485"
     CDS             complement(14776..15609)
                     /gene="ppsR"
                     /locus_tag="OO357_002485"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="posphoenolpyruvate synthetase regulatory
                     kinase/phosphorylase PpsR"
                     /protein_id="HCR5887398.1"
                     /translation="MDNAVDRHVFYISDGTAITAEVLGHAVMSQFPVTISSITLPFVE
                     NESRARAVKDQIDAIYHQTGVRPLVFYSIVLPEIRAIILQSEGFCQDIVQALVAPLQQ
                     EMKLDPTPIAHRTHGLNPNNLNKYDARIAAIDYTLAHDDGISLRNLDQAQVILLGVSR
                     CGKTPTSLYLAMQFGIRAANYPFIADDMDNLVLPASLKPLQHKLFGLTIDPERLAAIR
                     EERRENSRYASLRQCRMEVAEVEALYRKNQIPWINSTNYSVEEIATKILDIMGLSRRM
                     Y"
     gene            15941..18319
                     /gene="ppsA"
                     /locus_tag="OO357_002486"
     CDS             15941..18319
                     /gene="ppsA"
                     /locus_tag="OO357_002486"
                     /EC_number="2.7.9.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate synthase"
                     /protein_id="HCR5887399.1"
                     /translation="MSNNGSSPLVLWYNQLGMNDVDRVGGKNASLGEMITNLSGMGVS
                     VPNGFATTADAFNQFLDQSGVNQRIYELLDKTDIDDVTQLAKAGAQIRQWIIDTPFQP
                     ELENAIREAYAQLSADDENASFAVRSSATAEDMPDASFAGQQETFLNVQGFDAVLVAV
                     KHVFASLFNDRAISYRVHQGYDHRGVALSAGVQRMVRSDLASSGVMFSIDTESGFDQV
                     VFITSAWGLGEMVVQGAVNPDEFYVHKPTLAANRPAIVRRTMGSKKIRMVYAPTQEHG
                     KQVKIEDVPQEQRDIFSLTNEEVQELAKQAVQIEKHYGRPMDIEWAKDGHTGKLFIVQ
                     ARPETVRSRGQVMERYTLHSQGKIIAEGRAIGHRIGAGPVKVIHDISEMNRIEPGDVL
                     VTDMTDPDWEPIMKKASAIVTNRGGRTCHAAIIARELGIPAVVGCGDATERMKDGENV
                     TVSCAEGDTGYVYAELLEFSVKSSSVETMPDLPLKVMMNVGNPDRAFDFACLPSEGVG
                     LARLEFIINRMIGVHPRALLEFDDQEPQLQNEIREMMKGFDSPREFYVGRLTEGIATL
                     GAAFYPKRVIVRLSDFKSNEYANLVGGERYEPDEENPMLGFRGAGRYVSDSFRDCFAL
                     ECEAVKRVRNDMGLTNVEIMIPFVRTVDQAKAVVEELARQGLKRGENGLKIIMMCEIP
                     SNALLAEQFLEYFDGFSIGSNDMTQLALGLDRDSGVVSELFDERNDAVKALLSMAIRA
                     AKKQGKYVGICGQGPSDHEDFAAWLMEEGIDSLSLNPDTVVQTWLSLAELKK"
     gene            complement(18376..20022)
                     /gene="fadK"
                     /locus_tag="OO357_002487"
     CDS             complement(18376..20022)
                     /gene="fadK"
                     /locus_tag="OO357_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310435.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="medium-chain fatty-acid--CoA ligase"
                     /protein_id="HCR5887400.1"
                     /translation="MKVTLTFNEQRRAAYRQQGLWGDASLADYWQQTARAMPDKIAVV
                     DNHGASYTYSALDHAASCLANWMLAKGIESGDRIAFQLPGWCEFTVIYLACLKIGAVS
                     VPLLPSWREAELVWVLNKCQAKMFFAPTLFKQTRPVDLILPLQNQLPQLQQIVGVDKL
                     APATSSLSLSQIIADNIPLTTAITTHGDELAAVLFTSGTEGLPKGVMLTHNNILASER
                     AYCARLNLTWLDVFMMPAPLGHATGFLHGVTAPFLIGARSVLLDIFTPDACLALLEQQ
                     RCTCMLGATPFVYDLLNLLEKQPADLSALRFFLCGGTTIPKKVARECQQRSIKLLSVY
                     GSTESSPHAVVNLDNSLSRFMHTDGYAAAGVEIKVVDDARKTLPPGCEGEEASRGPNV
                     FMGYFDEPELTARALDEEGWYYSGDLCRMDEAGYIKITGRKKDIIVRGGENISSREVE
                     DILLQHPKIHDACVVAMPDERLGERSCAYVVLKAPHHSLSLEEMAAFFSRKRVAKYKY
                     PEHIVVIEKLPRTASDKIQKFLLRKDIIRRLTQDVCEEIE"
     gene            complement(20079..20372)
                     /locus_tag="OO357_002488"
     CDS             complement(20079..20372)
                     /locus_tag="OO357_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HCR5887401.1"
                     /translation="MSQNATVNVDIKLGVNKFHVDEGHPHIILTENPDINEFRKLMKA
                     CPAGLYKQDDVGNIHFDPAGCLECGTCRVLCGNTILEQWQYPAGTFGIDFRYG"
     gene            complement(20369..21658)
                     /locus_tag="OO357_002489"
     CDS             complement(20369..21658)
                     /locus_tag="OO357_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HCR5887402.1"
                     /translation="MSDDKFDAIVVGAGVAGSVAALVMARAGLDVLVIERGDSAGCKN
                     MTGGRLYAHTLEAIIPGFAVSAPVERKVTREKISFLTEESAVTLDFHREQPDVPQHAS
                     YTVLRNRLDPWLMEQAEQAGAQFIPGVRVDALVREGNKVTGVQAGDDILEANVVILAD
                     GVNSMLGRSLGMVPASDPHHYAVGVKEVIGLTPEQINDRFNITGEEGAAWLFAGSPSD
                     GLMGGGFLYTNKDSISLGLVCGLGDIAHAQKSVPQMLEDFKQHPAIRPLISGGKLLEY
                     SAHMVPEGGLAMVPQLVNEGVMIVGDAAGFCLNLGFTVRGMDLAIASAQAAATTVIAA
                     KERADFSANSLAQYKRELEQSCVMRDMQHFRKIPALMENPRLFSQYPRMVADIMNEMF
                     TIDGKPNQPVRKMIMGHAKKIGLINLLKDGIKGATAL"
     gene            complement(21714..22652)
                     /locus_tag="OO357_002490"
     CDS             complement(21714..22652)
                     /locus_tag="OO357_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit alpha"
                     /protein_id="HCR5887403.1"
                     /translation="MSQLNSVWVFSDNPERYAELFGGAQQWGQQVYAIVQNTDQAQAV
                     MPYGPKCIYVLEQNDALQRTENYAESIAALLKDKHPAMLLLAATKRGKALAARLSVQL
                     NAALVNDATAVDIVDGHICAEHRMYGGLAFAQEKINSPLAIITLAPGVQEPCTSDTSH
                     QCPTETVPYVAPRHEILCRERRAKAASSVDLSKAKRVVGVGRGLAAQDDLKMVHELAA
                     VLNAEVGCSRPIAEGENWMERERYIGVSGVLLKSDLYLTLGISGQIQHMVGGNGVKVI
                     VAINKDKNAPIFNYADYGLVGDIYKVVPALISQLSR"
     gene            complement(22672..23436)
                     /locus_tag="OO357_002491"
     CDS             complement(22672..23436)
                     /locus_tag="OO357_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein"
                     /protein_id="HCR5887404.1"
                     /translation="MKIITCFKLVPEEQDIVVTPEYTLNFDNADAKISQFDLNAIEAA
                     SQLATDDDEIAALTVGGSLLQNSKVRKDVLSRGPHSLYLVQDAQLEHALPLDTAKALA
                     AAIEKIGFDLLIFGEGSGDLYAQQVGLLVGEILQLPVINAVSAIQRQGNTLVIERTLE
                     DDVEVIELSVPAVLCVTSDINVPRIPSMKAILGAGKKPVNQWQASDIDWSQSAPLAEL
                     VGIRVPPQTERKHIILDNDSPEAIAELAEHLKKALN"
     gene            23756..>24229
                     /locus_tag="OO357_002492"
     CDS             23756..>24229
                     /locus_tag="OO357_002492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HCR5887405.1"
                     /translation="MYQRCFDNASETLFVAGKTPRLSRFAFSDDPKWESGHHVHDNET
                     ELIYVKKGVARFTIDSSLYVAHADDIVVIERGRLHAVASDVNDPATTCTCALYGFQFQ
                     GAEENQLLQPHSCPVIAAGQGKEVIKTLFNELSVILPQSKNSQTSSLWDALAYTLA"
BASE COUNT         5981 a         5896 c         6383 g         5969 t
ORIGIN      
        1 atgattaatt attatcgcca gaacgaaatg tgcacgaaaa attgttataa gaattgtgtc
       61 acttttatca ttgtgatatg accacttcgt cattaatctc atgagcgttt aaaatactac
      121 cgccaaatca cgcaacgaac tttgctggat tcagtttatg ctgctgtaaa tccgctcgca
      181 gtaaaccttt cagacgcacc gtgatgttat cagtggttct ttaagcgttt tgctggtgta
      241 ctcactacaa acgaattgcg aatcgaatca atgtgaaacg gaaaggtaca atctcccttt
      301 ttttagtgtt aacatcgttc aaccgggtgg aaggtgagag gttaatgtct ttcatttgtg
      361 tacctgattg cgaaccaatt tagcatttgt tggctagatg gtttcgcaat gaactgttaa
      421 taaacaaatt tttctttgta tgtgatcttt cgtgtgggtc accactgcaa ataaggatat
      481 aaaatgcctg ttataactct tcctgatggc agccaacgcc attacgacca cgctgtaagc
      541 cccatggatg ttgcgctgga cattggtcca ggtctggcga aagcctgtat cgcagggcgc
      601 gttaatggcg aactggttga tgcttgcgat ctgattgaaa acgacgcaca actgtcgatc
      661 attaccgcca aagacgaaga aggtctggag atcattcgtc actcctgtgc gcacctgtta
      721 gggcatgcga ttaaacaact ttggccgcat accaaaatgg caatcggccc ggttattgac
      781 aacggttttt attacgacgt tgatcttgac cgcacgttaa cccaggaaga tgtcgaagca
      841 ctcgagaagc ggatgcatga gcttgctgag aaaaactacg acgtcattaa gaagaaagtc
      901 agctggcacg aagcgcgtga aactttcgcc aaccgtgggg agagctacaa agtttccatt
      961 cttgacgaaa acatcgccca tgatgacaag ccaggtctgt acttccatga agaatatgtc
     1021 gatatgtgcc gcggtccgca cgtaccgaac atgcgtttct gccatcattt caaactaatg
     1081 aaaacggcag gggcttactg gcgtggcgac agcaacaaca aaatgttgca acgtatttac
     1141 ggtacggcgt gggcagacaa aaaagcactt aacgcttacc tgcagcgcct ggaagaagcc
     1201 gcgaaacgcg accaccgtaa aatcggtaaa cagctcgacc tgtaccatat gcaggaagaa
     1261 gcgccgggta tggtattctg gcacaacgac ggctggacca tcttccgtga actggaagtg
     1321 tttgttcgtt ctaaactgaa agagtaccag tatcaggaag ttaaaggtcc gttcatgatg
     1381 gaccgtgtcc tgtgggaaaa aaccggtcac tgggacaact acaaagatgc aatgttcacc
     1441 acatcttctg agaaccgtga atactgcatt aagccgatga actgcccggg tcacgtacaa
     1501 attttcaacc aggggctgaa gtcttatcgc gatctgccgc tgcgtatggc cgagtttggt
     1561 agctgccacc gtaacgagcc gtcaggttcg ctgcatggcc tgatgcgcgt gcgtggattt
     1621 acccaggatg acgcgcatat cttctgtact gaagaacaaa ttcgcgatga agttaacgga
     1681 tgtatccgtt tagtctatga tatgtacagc acttttggct tcgagaagat cgtcgtcaaa
     1741 ctctccactc gtcctgaaaa acgtattggc agcgacgaaa tgtgggatcg tgctgaggcg
     1801 gacctggcgg ttgcgctgga agaaaacaac atcccgtttg aatatcaact gggtgaaggc
     1861 gctttctacg gtccgaaaat tgaatttacc ctgtatgact gcctcgatcg tgcatggcag
     1921 tgcggtacag tacagctgga cttctccttg ccgtctcgtc tgagcgcctc ctatgtgggc
     1981 gaagacaacg agcgtaaggt accggtaatg attcaccgcg caattctggg gtcgatggag
     2041 cgtttcatcg gtatcctgac cgaagaattc gctggtttct tcccgacctg gcttgcgcca
     2101 gttcaggttg ttatcatgaa tattaccgat tcacagtctg aatacgttaa cgaattgacg
     2161 caaaaactat caaatgcggg cattcgtgtt aaagcagact tgagaaatga gaagattggc
     2221 tttaaaatcc gcgagcacac tttgcgtcgc gtcccatata tgctggtctg tggtgataaa
     2281 gaggtggaat caggcaaagt tgccgttcgc acccgccgtg gtaaagacct gggaagcatg
     2341 gacgtaaatg aagtgatcga gaagctgcaa caagagattc gcagccgcag tcttaaacaa
     2401 ttggaggaat aaggtattaa aggcggaaaa cgagttcaaa cggcgcgccc taaccgtatc
     2461 aatggcgaaa ttcgcgccca ggaagttcgc ttaacaggtc tggaaggcga gcagcttggt
     2521 attgtgagtc tgagagaagc tctggagaaa gcagaagaag ccggagtaga cttagtcgag
     2581 atcagcccta acgccgagcc gccggtttgt cgtataatgg attacggcaa attcctctat
     2641 gaaaagagca agtcttctaa ggaacagaag aaaaagcaaa aagttatcca ggttaaggaa
     2701 attaaattcc gtcctagtac agatgaaggc gactatcagg taaaactccg cagcctgatt
     2761 cgctttctcg aagagggtga taaagccaaa atcacgctgc gtttccgcgg tcgtgagatg
     2821 gcgcaccagc aaatcggtat ggaagtgctt aatcgcgtga aagacgattt gcaagaactg
     2881 gcagtagtcg aatccttccc aacgaagatc gaaggccgcc agatgatcat ggtgctcgct
     2941 cctaagaaga aacagtaagg ccttcaagta gcactatctg tggagccttt gggcttcaca
     3001 ggcattgttc gcctttgttt cgtttattaa caatgcgaag tggaagttat taaaatgcca
     3061 aaaattaaga ccgtacgcgg tgctgctaag cgcttcaaaa aaaccggtaa aggtggtttt
     3121 aagcacaagc acgctaacct gcgtcacatt ctgactaaaa aagcgaccaa acgtaaacgt
     3181 cacctgcgtc cgaaagccat ggtttccaaa ggcgatctgg gcctggtaat cgcgtgcctg
     3241 ccgtacgcat aagccgttaa cgtttttaac tttttaatta gaatatagat acaggagagc
     3301 acatatggct cgcgtaaaac gtggtgttat tgcacgcgca cgtcacaaga aaattttgaa
     3361 acaagctaaa ggctactacg gtgcgcgttc tcgcgtatac cgcgttgcct tccaggctgt
     3421 tatcaaagct ggtcagtatg cttaccgtga ccgtcgtcaa cgtaagcgtc agttccgtca
     3481 actgtggatt gcgcgtatca acgcagcagc acgtcagaac ggtatttctt acagcaaatt
     3541 catcaatggc ctgaaaaaag cctctgttga aatcgaccgt aagatcctgg ctgatatcgc
     3601 agtattcgac aaagtagcgt tcaccactct ggttgaaaaa gcgaaagcag ctctggcata
     3661 agccagttga aagagggagc tagtctccct cttttcgttt caacgccatc aaaacattga
     3721 cttttatcgc cgtagccttt tcaataaagg tcttttgaag agtaccaaaa ggtaacgcaa
     3781 gcaatgaatg ctgctatttt ccgcttcttt ttttacttta gcacctgaat ccaggaggct
     3841 agcgcgtgag aagagaaacg gaaaacagcg cctgaaagcc tcccagtgga ggcttttttt
     3901 gtatgcgcgt tttgaaaaat aaaaagccag tctatcaggc gtaattgtcg cagccagtct
     3961 ggacacggcc agtgcggaga aaccggagcg tacttaagta cgtgaggatt tcgagcactg
     4021 cccgggacca aaatggcaag taaaatagcc tgatgggata ggctctaagt ccaacgaacc
     4081 agtgtcacca ctgacacaat gaggaaaacc atgtcacatc tcgcagaact ggttgccagt
     4141 gcgaaggcgg ccattagcca ggcgtcagat gttgccgcgt tagataatgt gcgcgtcgaa
     4201 tatttgggta aaaaagggca cttaaccctt cagatgacga ccctgcgtga gctgccgcca
     4261 gaagagcgtc cggcagctgg tgcggttatc aacgaagcga aagagcaggt tcagcaggcg
     4321 ctgaatgcgc gtaaagcgga actggaaagc gctgcactga atgcgcgtct ggcggcggaa
     4381 acgattgatg tctctctgcc aggtcgtcgc attgaaaacg gcggtctgca tccggttacc
     4441 cgtaccatcg accgtatcga aagtttcttc ggtgagcttg gctttaccgt ggcaaccggg
     4501 ccggaaatcg aagacgatta tcataacttc gatgctctga acattcctgg tcaccacccg
     4561 gcgcgcgctg accacgacac tttctggttt gacgctaccc gcctgctgcg tacccagacc
     4621 tctggcgtac agatccgcac catgaaagcc cagcagccac cgattcgtat catcgcgcct
     4681 ggccgtgttt atcgtaacga ctacgaccag actcacacgc cgatgttcca tcagatggaa
     4741 ggtctgattg ttgataccaa catcagcttt accaacctga aaggcacgct gcacgacttc
     4801 ctgcgtaact tctttgagga agatttgcag attcgcttcc gtccttccta cttcccgttt
     4861 accgagcctt ctgcagaagt tgacgtcatg ggtaaaaacg gtaaatggct ggaagtgctg
     4921 ggctgcggga tggtgcatcc gaacgtgctg cgtaacgtta gcatcgaccc ggaagtttac
     4981 tctggtttcg ccttcgggat ggggatggag cgtctgacta tgttgcgtta cggcgtcacc
     5041 gacctgcgtt cattcttcga aaacgatctg cgtttcctca aacagtttaa ataaggcagg
     5101 aatagattat gaaattcagt gaactgtggt tacgcgaatg ggtgaacccg gcgatcgata
     5161 gcgatacgct ggcgaatcaa atcactatgg cgggcctgga agttgacggt gtagaaccgg
     5221 ttgccggtag cttccacggc gtggtcgttg gtgaagtggt tgagtgtgcg cagcatccga
     5281 acgctgacaa actgcgtgtg acaaaagtga atgtcggcgg cgatcgcctg ctggacatcg
     5341 tctgcggtgc gccaaactgc cgtcagggcc tgcgtgtggc ggtagctacc attggtgctg
     5401 ttctgccggg tgatttcaaa attaaagcgg cgaaactgcg tggcgaaccg tctgaaggga
     5461 tgctgtgctc cttttctgag ctgggaattt ctgacgatca cagtggcatt atcgaactgc
     5521 ctgcggatgc gccgattggc accgacatcc gcgaatacct gaaactcgat gataacacca
     5581 tcgaaatcag cgtgacacca aaccgtgccg actgcttagg tatcattggt gttgcgcgtg
     5641 acgttgccgt gctgaatcag ctgccgctgg ttgaaccgga aatcgttccg gttggtgcga
     5701 ctatcgacga cacgttgccg attacagtcg aagcgccgga agcctgcccg cgctatcttg
     5761 gccgtgtggt aaaaggcatt aacgttaaag cgccaactcc gctgtggatg aaagaaaaac
     5821 tgcgtcgttg cgggatccgt tctatcgatg cagttgttga cgtcaccaac tatgtactgc
     5881 tcgaactggg ccagccgatg cacgctttcg ataaagatcg cattgaaggc ggcattgtgg
     5941 tgcggatggc aaaagagggc gaaacgctgg tgctgcttga tggcactgaa gcgaagctga
     6001 atgctgacac tctggtcatc gccgaccaca acaaggcgct ggcgatgggc ggcatctttg
     6061 gtggcgaaca ctctggcgtg aatgacgaaa cgcaaaacgt cttgctggaa tgcgctttct
     6121 tcagcccgct gtctatcacc ggtcgtgctc gtcgtcatgg cctgcatact gatgcgtctc
     6181 accgttatga gcgtggcgtt gatccggtac tgcagcacaa agcgatggaa cgtgcgaccc
     6241 gtctgctgat cgacatctgc ggtggtgagg ctggcccggt aattgatatc accaacgaag
     6301 caacgctgcc gaagcgtgca accatcactc tacgtcgtag caaactggat cgcctgatag
     6361 gccatcatat tgctgatgag caggtaactg acattctgcg tcgtctcggc tgcgaagtga
     6421 ccgaaggcaa agacgaatgg caggcagttg cgccgagctg gcgtttcgat atggagattg
     6481 aagaagatct ggtcgaagaa gtcgcgcgtg tttacggcta caacaacatc ccggatgagc
     6541 cggtacaggc aagcctgatt atgggtactc accgtgaagc tgacctgtcg ctcaagcgcg
     6601 tgaaaacgct gctcaacgac aaaggctatc aggaagtgat cacctacagc ttcgttgatc
     6661 cgaaagtgca gcagatgatc catccaggcg ttgaagcctt actgctggca agcccgatct
     6721 ctgttgaaat gtcagcaatg cgtctttctc tgtggaccgg cctgctggca accgtggtgt
     6781 acaaccagaa ccgtcagcag aaccgtgtgc gcattttcga aagcggtctg cgtttcgtac
     6841 cagatactca ggcaccgttg ggcattcgtc aggatctgat gttagccggt gtgatttgcg
     6901 gtaaccgtta cgaagagcac tggaacctgg caaaagagac cgttgatttc tatgatttga
     6961 aaggcgacct tgaatccgtt ctcgacctga ccggtaaact gaatgaggtt gagttccgtg
     7021 cagaagcgaa tccggcactg catccggggc aatccgcagc gatttatctg aaaggtgaac
     7081 gtattggttt tgttggagtt gttcatcctg aactggaacg taaactggat cttaacggtc
     7141 gcactctggt gttcgaactg gagtggaaca agctcgcaga ccgcgtggtg cctcaggcgc
     7201 gcgagatttc tcgcttcccg gcgaaccgtc gtgacatcgc ggtggtggtc gcagaaaacg
     7261 ttcccgcagc ggatatttta tccgaatgta agaaagttgg cgtaaatcag gtagttggcg
     7321 taaacttatt tgacgtgtac cgcggtaagg gtgttgcgga ggggtataag agcctcgcca
     7381 taagcctgat cctgcaagat accagccgta cactcgaaga agaggagatt gccgctaccg
     7441 tcgccaaatg tgtagaggca ttaaaagagc gattccaggc atcattgagg gattgaacct
     7501 atggcgctta caaaagctga aatgtcagaa tatctgtttg ataagcttgg gcttagcaag
     7561 cgggatgcca aagaactggt tgaactgttt ttcgaagaga tccgtcgcgc tctggaaaac
     7621 ggcgaacagg tgaaactctc tggttttggt aacttcgatc tgcgtgataa gaatcaacgc
     7681 ccgggacgta acccgaaaac gggcgaggat attcccatta cagcacggcg cgtggtgacc
     7741 ttcagacccg ggcagaagtt aaaaagccgg gtcgaaaacg cttcgcccaa agacgagtaa
     7801 tctgatctaa ctaaaaaggc cgctatgcgg ccttttttct tttcactgtc gaagagtcac
     7861 cgtaaaatca acgccatgac acttcagcag aacggatacc atgctgacac ttgcccgcca
     7921 acaacagcga caaaatattc gctggttatt atgcctgtca gttttgatgc tgctggcgct
     7981 tctcttaagc ctttgcgccg gtgaacaatg gatttcgcca ggtgactggt ttactcctcg
     8041 tggcgaactg ttcgtctggc aaattcgcct gccacgtacg ctggctgtat tgctggttgg
     8101 tgcggcgctg gctatatccg gcgctgtaat gcaggcgttg tttgaaaatc ctctggcaga
     8161 acctggacta cttggcgtct ctaacggcgc aggcgtgggg cttatcgccg cggtattgct
     8221 tgggcaaggg caactcccca actgggcgct agggctgtgt gcgattgctg gcgcgcttat
     8281 catcacttta atactcttac gtttcgcccg tcgtcatctt tcgaccagtc ggttattgct
     8341 ggctggcgtt gcattaggga ttatctgcag cgcactaatg acgtgggcta tctacttttc
     8401 cacctctgtt gatttacgtc agctgatgta ctggatgatg ggcggttttg gcggcgtaga
     8461 ctggcggcaa agctggctga tgctggcatt gatccccgtg ttgttgtgga tctgttgtca
     8521 gtccaggccg atgaatatgt tagcgcttgg cgagatctcg gcgcggcaac tgggtttacc
     8581 catgtggttc tggcgcaatg tgctggtggc agcgaccggc tggatggttg gcgtcagtgt
     8641 ggcgctggcg ggtgctatcg gctttattgg tctggtgatc ccacatattc ttcggttgtg
     8701 tggtttaacc gatcatcgcg tattacttcc cggctgcgcg ctggcagggg cgagcgcatt
     8761 gctgctggcc gatattgtag cgcgcctggc attagctgcc gcagagctgc ctattggcgt
     8821 ggtcaccgca acgttgggtg cgccggtgtt tatctggtta ttgttaaaag caggacgtta
     8881 gccgcaaaaa gacggtctat gattaaaagc taagattttt actgaccaca cctaggagaa
     8941 acgatgcaag attccattct gacgaccgta gtgaaagata tcgacggtga agtgaccacg
     9001 ctggagaagt acgccggtaa tgtgctgtta attgtcaatg tcgcctcaaa gtgtggctta
     9061 acgccgcaat atgagcagtt ggagaatatt caaaaagcct gggccgatcg aggttttgtg
     9121 gtgctgggat tcccgtgcaa ccagtttctg gaacaagaac cgggcagcga tgaagagatt
     9181 aaaacttact gtaccaccac atggggggtg acgttcccga tgttcagtaa gattgaagtt
     9241 aatggcgaag gacgccatcc gctgtatcaa aaattgattg ccgcagcgcc gaccacagtc
     9301 gcgccggaag agagcggatt ctatgcccgt atggtcagca aaggccgtgc accgctgtac
     9361 ccggatgata ttttatggaa ttttgaaaaa ttcctggttg gcagggacgg aaaagtcatc
     9421 cagcgttttt ccccggatat gacgccggaa gatcccattg tgatggaaag cattaaactg
     9481 gcgttggcaa aataatgtct attgtgatgc agttacagga tgttgcggaa tctacccgcc
     9541 tggggccgct ttcaggcgag gttcgggctg gggaaatcct gcacctggtg gggccgaatg
     9601 gcgcgggtaa gagtacctta ctggcgcgaa tggccggaat gaccagcggt aagggaagca
     9661 ttcagttcgc ggggcaacca ctggaagcat ggtccgcaac aaaactcgcg ctgcatcgcg
     9721 cctatctttc acaacagcag acgccgccgt ttgcaatgcc ggtctggcac tacctgacac
     9781 tgcatcagca cgataaaacg cgtaccgaac tactgaatga tgtcgcaggg gcgctggctc
     9841 ttgatgacaa actcggtcgt agcaccaatc aactttccgg cggtgaatgg caacgcgtac
     9901 gtcttgctgc ggtggtgttg caaatcacac cacaagccaa tcccgcaggc caattgctgc
     9961 ttcttgatga gccgatgaac agtcttgatg ttgcgcaaca aagtgcgtta gacaaaattc
    10021 tgagcgcgct gtgtcagcaa ggactggcga ttgtgatgag cagtcacgat ctcaaccaca
    10081 cgttgcgtca tgcgcatcgg gcgtggttgc taaaaggtgg aaaaatgctg gccagtggac
    10141 gcagagaaga ggtgctcacg ccgccaaatc tggcgcaggc ctatgggatg aattttcgcc
    10201 gtctggatat cgaaggtcac agaatgctga tttcgaccat ctgatttcat tagtatcccg
    10261 aaaatcgggt aattacgacg ctaaattaat gcaagaataa aaaacagagg aaccgtacgg
    10321 aatgcgtttc tgccttattt tgatcacagc actgtttctg gccgggtgta gccaccataa
    10381 agcaccgccg ccaaatgcca gactttctga ttcgattacc gttattgccg gtttgaacga
    10441 ccagctacaa agctggcatg gcacgccgta tcgttatggt ggcatgacgc ggcgcggtgt
    10501 ggactgttcg ggatttgtgg ttgtgacgat gcgcgatcgt ttcgatttgc atctgccccg
    10561 tgaaaccaaa caacaagcct ctattggcac gcaaattgat aaagacgagt tgctgcctgg
    10621 tgacctggtc tttttcaaaa cgggttccgg acaaaatggt ttgcatgtag gtatttacga
    10681 taccaacaac caatttatcc acgcctctac cagcaaggga gtgatgcgtt cctcacttga
    10741 taatgtctat tggcagaaaa atttctggca ggcgagacga atctagtatg tcgaaaaatt
    10801 gacggcgatg atacatcgcc gtcaatttac tcctgaatac agtagtattt tgcagacaaa
    10861 aattccgtaa atggattatt cgtttataat caggataatc taaaaaaagg ccccctgaaa
    10921 aagtggttaa catggaacaa atgaaagtta ctccttctgt aattgttatg tcttatggcc
    10981 tcatcccttt tagccggata ctgaaaaaca tccttcgaga gggacggtta ccatgaagat
    11041 ttttttggag aatctttatc attctgattg ctatttcctg ccgataagag ataaccagca
    11101 actccttgtc ggcgttgagt taattaccca tttctccagt gaagatggca cggttcgtat
    11161 cccgacgagt cgggttatag cgcaacttac cgaggagcaa cattggcaat tattttcaga
    11221 gcagttggaa ttactaaaat catgccaaca cttttttatt caacataaac tctttgcctg
    11281 gcttaattta acgccacaag tggctacatt attattagac agggataatt acgcaggcga
    11341 actattaaaa tatccgttta ttgagctgtt gattaatgag aattacccgc atctcaatga
    11401 aggaaaagat aatcgcgatc ttttgtcctt atcgcaaatg tatccccttg tgttaggcaa
    11461 tttgggagca ggtaatagca caatgaaagc ggtttttgat ggtcttttca cccgcgtaat
    11521 gctggagaaa ggttttattc agcaacaaat cacgcatcgc tccttcgaac cgtttattcg
    11581 tgcgatccag gcgcaaattt ccccttgttg caattgcatc atcgcggggg gcattgatac
    11641 gccagaaata ttggcacaga ttaccccctt cgatttccat gctctccagg gatgcctgtg
    11701 gcctgccgtt cctataaatc agataacgac gttggttcag cgataaccct cctgtttgct
    11761 ggtgtttaag acgagagtaa ccgtctacac tatcaaacag ataggatcta tgaccctgtc
    11821 ttttattacc cgctggcgcg atgaattgcc agaaacctat acagcacttt cccctacgcc
    11881 tttaaataat gcccggctga tttggcataa taccgaactc gctaacacgc tgagtattcc
    11941 atcgtcgctg tttaaaaatg ccgcaggtgt ctggggcggc gaaaccctac tgcctggtat
    12001 gtcaccactg gcacaggttt acagtggtca tcagttcgtc gtctgggcgg gccaactggg
    12061 tgatgggcgc ggcattttac ttggcgaaca actgcttgct gatggcacta caatggactg
    12121 gcatctgaaa ggtgctggcc tgacgcctta ttcgcgaatg ggtgatggac gggcggtttt
    12181 acgttcgacg atacgagaaa gtctcgccag tgaggcgatg cattatctgg gcattccgac
    12241 gacccgcgcg ttaagtatcg tcaccagcga ttcgccagtg tatcgggaaa cggtggagcc
    12301 aggcgcgatg ctgatgcgtg tggcaccaag tcatctgcgc tttggtcatt tcgaacattt
    12361 ttactatcgc cgcgagccgg aaaaggttcg tcagttggct gactttgcca ttcgtcatta
    12421 ctggtcacat cttgaagacg atgaggacaa ataccgtctc tggtttaacg atgttgtcgc
    12481 acgtaccgcg tcgttaattg cccaatggca gacggtcggc tttgcacatg gggtgatgaa
    12541 taccgacaac atgtcgctgc tgggactgac gcttgattac gggccgtttg gttttcttga
    12601 tgattacgaa cccggtttta tttgtaatca ctcggatcat caagggcgtt atagctttga
    12661 taatcaacct gctgtcgcgt tgtggaattt acagcgtctg gcgcagacat tgtcaccatt
    12721 tgttgccgta gatgccctga atgaggccct ggacagctat cagcaggttt tgttgacgca
    12781 ttatggacaa cggatgcggc agaaactggg cttcatgacg gagcaaaaag aggataacgc
    12841 gctactgaat gaattattca gtctgatggc gcgagagcgc agcgattata cccgcacatt
    12901 ccgcatgctg agtctgaccg agcagcacag cgcggcgtca ccgctacgtg aggagtttat
    12961 tgatcgtgcg gcatttgatg actggtttgc ccgttatcgg gggcgtttgc aacaagacga
    13021 ggttagcgat agtgagcgtc agcaactgat gcaaagcgtt aacccagctc tggtattgcg
    13081 caactggttg gcgcaacggg cgattgaagc ggcagaaaag ggtgatatga tggaattgca
    13141 ccgcctgcat gaggcgttgc gaaatccttt cagcgacaga gatgatgact atgtcagtcg
    13201 tccacctgac tggggtaaac ggctggaagt cagctgctcg agttaaaggc tacttagtta
    13261 acagcagctt gccagcctga gttttacgga gcagatattc ttgcccgtca tgatcgataa
    13321 tcagtttgcc atccggacct aacagtgtct ggctggtaat tcgccggatg ggctgcgggg
    13381 aagcggtctt gtgattggca tccgtttcag gcgtgagttt tctgctatcc gtataacgca
    13441 taaaaattaa taataattga tataacaatg attcctatta tcaatattat ggcagaccag
    13501 agcaagcagt aatgtgttga gaagacaaca agttgcgcag aaatgcccat cccatcagga
    13561 atgggcacgc attcagaagc gggtatctac cgcaaaggcg agtttttcga ccaggcgttc
    13621 ggtatcttcc cagcccagac acgggtcggt aatggattga ccgtaagtga gcggctgacc
    13681 gccgacgatt ttttgcgttc cttcgcgcag gaaactttcc gccataattc cagcaatctc
    13741 cgtagagcca ttgcggattt gctgacaaat atcctcacaa acttctaact ggcgacggtg
    13801 ctgcttctgg cagttaccgt ggctgaaatc caccaccaga tgttcaggta aatcaaactc
    13861 gtgcagcgta tcgcaggctg cggcgatatc atcggcatga taattcggtt ttttgccgcc
    13921 acgcataata atgtggccat acgggttgcc gctggtctga tagatggtca tctgaccatt
    13981 tttgtctggc gagaggaaca tatggctggc gcgggctgcg cggatagcat ccacggcaat
    14041 ccgcgtgttg ccatcggtac catttttaaa acctaccgga caggagagtg ctgaagccat
    14101 ttcgcggtgg atctgacttt cggtagtacg agcgccaatc gcgccccaac tgattaaatc
    14161 agcaataaac tgaccggtta ccatatcgag gaactcagtc gcggtaggga cacccagctc
    14221 atttacctgt aaaagtaatt tgcgcgccag ctccagaccg tgatttaccc gatagctgcc
    14281 gtttaaatcc ggatcggaga ttagcccttt ccagccgaca acagttcgtg gtttttcaaa
    14341 ataggtgcgc attacgattt ccagccgtga ctggtactgg ttgcgcagcg actgcagacg
    14401 ggtggcgtac tccattgcag cggtgagatc gtggatcgag caggggccaa taatgaccaa
    14461 cagtcgctta tcttcaccat tcagtatttt ttcaattctg cggcgggagt cggtgacatg
    14521 ggtggcgacg ccaggcgtta cgggataccg tagcgcgagt tcggcgggcg ttaccaggct
    14581 ctcaatacgc gcagtacgga gttcgtcagt tctgttcatt acaagtctca gaaatttgtt
    14641 gcttctacgg caggactacc ggaaagtgat gcgcatcacg ataaaccaat cgcagctgat
    14701 ttcaagtgcg aggtgtgtca cacctcgccg ggtggttagc atgataacaa aaaaacaaac
    14761 caatgcacta gttctctagt acattcggcg actaaggccc atgatatcga ggatcttggt
    14821 ggcaatctct tctaccgaat aattggtact gttaatccac gggatctgat ttttacggta
    14881 caaggcttcc acttccgcga cttccatcct gcactgacga agcgaggcat agcgactgtt
    14941 ctcccgacgt tcctcgcgaa tcgccgccag acgttccggg tcgatagtca ggccgaacaa
    15001 tttatgctga agcggtttga gcgacgcggg tagcaccaga ttatccatat cgtcggcaat
    15061 aaaggggtag tttgccgcgc ggataccaaa ttgcattgcc agatacagac tggtgggggt
    15121 tttaccacag cgagaaacac cgagcaggat cacctgagcc tgatccagat tgcgcaacga
    15181 aatgccgtca tcgtgggcga gggtgtaatc aatcgccgca atgcgcgcat catatttatt
    15241 gagattatta gggttaaggc catgggtacg atgagcaatc ggcgttggat ccagtttcat
    15301 ctcttgttgt agcggggcaa ccagcgcctg aacgatatcc tggcaaaagc cttcactttg
    15361 caagatgatg gcgcgaatct ccggcaacac gatggagtag aagaccagcg ggcgcacgcc
    15421 tgtctggtga taaattgcgt caatctgatc cttcactgcc cgtgcacggc tctcattttc
    15481 gacaaacggc agcgtgatgc tgctgatagt gacgggaaat tgtgacatta ctgcgtgtcc
    15541 taatacctcc gcagttattg ccgtaccatc agaaatataa aaaacgtggc gatcaacagc
    15601 attatccatt ttgttcttcc cgtgaagcag acataattct atgaaagcat aaattaaaag
    15661 cgcacagaag cgtaggacgt aatgtctggt ttataaaatg aaccttcatt ttatttttta
    15721 taaaaacagc atttcatttt tatggtttcg tttataccga tggtttatgt ggaaattgtc
    15781 gaatagagca ggtttgcgca acgctgggat cagtcttaaa aagtaaaaaa atatatttgc
    15841 ttgaacgatt caccgttttt ttcatccggt taaatatgca aagataaatg cgcagaaatg
    15901 tgtttctcaa accgttcatt tatcacaaaa ggattgttcg atgtccaaca atggctcgtc
    15961 accgctggtg ctttggtata accaactcgg catgaatgat gtagacaggg ttgggggcaa
    16021 aaatgcctcc ctgggtgaaa tgattactaa tctttccgga atgggtgttt ccgttccgaa
    16081 tggtttcgcc acaaccgccg acgcgtttaa ccagtttctg gaccaaagcg gcgtaaacca
    16141 gcgcatttat gaactgctgg ataaaacgga tattgacgat gttactcagc ttgcgaaagc
    16201 gggcgcgcaa atccgccagt ggattatcga cactcccttc cagcctgagc tggaaaacgc
    16261 catacgcgaa gcctatgcac agctttccgc cgatgacgaa aacgcctctt ttgcggtacg
    16321 ctcctccgcc accgcagaag acatgccgga cgcttccttt gccggtcagc aggaaacctt
    16381 cctcaacgtt cagggttttg acgccgttct cgtggcagtg aaacatgtat ttgcttctct
    16441 gtttaacgat cgcgccatct cttatcgtgt gcaccagggt tacgatcacc gtggcgtggc
    16501 gctctccgcc ggtgttcagc ggatggtgcg ctccgacctc gcatcttctg gcgtgatgtt
    16561 ctccattgat accgaatccg gctttgacca ggtggtgttt atcacttccg catggggcct
    16621 tggggaaatg gtcgtgcagg gtgcggttaa cccggatgag ttttacgtgc ataaaccgac
    16681 actggcggcg aatcgcccgg ctatcgtgcg ccgcaccatg gggtcgaaaa aaatccgcat
    16741 ggtttacgcg ccgacccagg agcacggcaa gcaggttaaa atcgaagacg taccgcagga
    16801 acagcgtgac atcttctcgc tgaccaacga agaagtgcag gaactggcaa aacaggccgt
    16861 acaaattgag aaacactacg gtcgcccaat ggatattgag tgggcgaaag atggccacac
    16921 cggcaaactg ttcattgtgc aggcgcgtcc ggaaaccgtg cgctcacgcg gtcaggtcat
    16981 ggagcgttat acgctgcatt cacagggtaa gattatcgcc gaaggccgtg ctatcggtca
    17041 tcgcatcggc gcgggtccgg tgaaagtcat ccatgacatc agcgaaatga accgcatcga
    17101 acctggcgac gtgctggtta ctgacatgac cgacccggac tgggaaccga tcatgaagaa
    17161 agcatctgcc atcgtcacca accgtggcgg tcgtacctgt cacgcggcga tcatcgctcg
    17221 tgaactgggc attccggcgg tagtgggctg tggagatgca acagaacgga tgaaagacgg
    17281 tgagaacgtc actgtttctt gtgccgaagg tgataccggt tacgtctatg cggagttgct
    17341 ggaatttagc gtgaaaagct ccagcgtaga aacgatgccg gatctgccgt tgaaagtgat
    17401 gatgaacgtc ggtaacccgg accgtgcttt cgacttcgcc tgcctgccga gcgaaggcgt
    17461 gggccttgcg cgtctggaat ttatcatcaa ccgtatgatt ggcgtccacc cacgcgcact
    17521 gcttgagttt gacgatcagg aaccgcagtt gcaaaacgaa atccgcgaga tgatgaaagg
    17581 ttttgattct ccgcgtgaat tttacgttgg tcgtctgact gaagggatcg cgacgctggg
    17641 tgccgcgttt tatccgaagc gcgtcattgt ccgtctctct gattttaaat cgaacgaata
    17701 tgccaacctg gtcggtggtg agcgttacga gccagatgaa gagaacccga tgctcggctt
    17761 ccgtggtgcg ggccgctatg tttccgacag cttccgcgac tgcttcgcac tggagtgtga
    17821 agcagtgaaa cgtgtgcgca acgacatggg gctgaccaac gttgagatca tgatcccgtt
    17881 cgtgcgtacc gtagatcagg cgaaagcggt ggttgaagaa ctggcgcgtc aggggctgaa
    17941 acgtggcgag aacgggctga aaatcatcat gatgtgtgaa atcccgtcca acgccttgct
    18001 ggccgagcag ttcctcgaat atttcgacgg cttctcaatt ggctcaaacg atatgacgca
    18061 gctggcgctc ggtctggacc gtgactccgg cgtggtgtct gaattgttcg atgagcgcaa
    18121 cgatgcggtg aaagcactgc tgtcgatggc tatccgtgcc gcgaagaaac agggcaaata
    18181 tgtcgggatt tgcggtcagg gtccgtccga ccacgaagac tttgccgcat ggttgatgga
    18241 agaggggatc gatagcctgt ctctgaaccc ggacaccgtg gtgcaaacct ggttaagcct
    18301 ggctgaactg aagaaataaa ataaatcccc ggcggcgttc agtcgccggg gttatgtgat
    18361 ccccgaagat gaaacttatt caatctcttc acagacatcc tgcgttaatc gccgtataat
    18421 atcttttctt aacaaaaact tttgtatttt atctgaggca gtgcgcggta gtttttcgat
    18481 taccacgata tgttcaggat atttatattt tgcgacccgt ttacggctaa aaaaagccgc
    18541 catctcttcc agcgataatg aatgatgcgg cgctttcagc acgacataag cgcatgatcg
    18601 ttcacctaaa cgttcatcgg gcattgcaac cacacaggca tcgtgaattt taggatgctg
    18661 caataaaata tcttccactt cacggctgct aatattttcg ccgccgcgga caataatatc
    18721 ttttttgcgt ccggtaattt ttatatagcc agcctcatcc atacggcaga gatcgccgct
    18781 gtaataccag ccttcttcat ccagcgcacg ggcggttaat tcaggttcat caaaataccc
    18841 cataaacaca ttggggccac gcgatgcttc ttcaccttcg caacctggcg gtaaggtctt
    18901 gcgtgcgtca tcgaccactt taatctctac acctgcggca gcgtaaccat cggtatgcat
    18961 aaagcgcgac aaagaattat cgagattcac caccgcatgc ggcgaacttt ctgtggaacc
    19021 ataaacactt aataatttaa tgctgcgctg ctggcattcg cgcgccactt ttttggggat
    19081 tgtggtaccg ccgcaaagaa agaagcgcag cgctgaaagg tctgcgggtt gtttctctag
    19141 taaattcaaa agatcataga caaacggcgt tgcgcctagc atacaggtgc aacgctgctg
    19201 ctcaagcagc gcgagacacg catcaggagt gaaaatatct aacaacacgc tgcgagcgcc
    19261 aattaagaat ggtgccgtta cgccatgcag aaagcccgtt gcgtgaccaa gtggcgcagg
    19321 catcataaag acatccagcc aggtcagatt cagccgcgcg caataagccc gctcactggc
    19381 gagaatattg ttatgcgtta gcatcacgcc ctttggcaga ccctcggttc cggaggtaaa
    19441 cagcaccgca gctaattcat cgccgtgggt cgttatcgcc gtggtcagtg ggatattgtc
    19501 ggcgataatc tgacttaatg agagggaaga ggtggcggga gccagtttgt ccacgccgac
    19561 aatttgttgt agttgtggaa gctgattttg cagcggcagg attaaatcta ccggacgcgt
    19621 ttgtttaaac aacgtcggtg caaagaacat ttttgcctga cacttattga gcacccacac
    19681 cagttctgct tcccgccagg aaggcaacag cggcacggaa accgcaccga ttttcaggca
    19741 ggcaagatag ataacggtaa attcacacca gccaggcagt tgaaatgcga tgcgatcgcc
    19801 tgattcaata cccttcgcta acatccagtt tgccagacag ctcgcggcgt gatcgagcgc
    19861 gctataggtg tacgatgcac catgattatc gaccacggca attttgtctg gcatcgcacg
    19921 agcggtctgc tgccagtaat cggccagtga agcatcgccc catagccctt gctgacgata
    19981 cgccgcacga cgttgttcgt taaacgttaa tgtcactttc attttggact ctcgaaacag
    20041 aacatcaggc ccgagttgcg ggcctgtggg atgcatgatt agccgtagcg aaaatcaata
    20101 ccgaaggtgc ctgcgggata ttgccactgt tcgagaatag tgttaccgca cagcacccga
    20161 caggtgccgc actccagaca accggcggga tcaaaatgaa tgtttcctac gtcatcctgc
    20221 ttataaagtc cggcagggca ggctttcatt aatttacgga attcattgat atcgggattt
    20281 tccgtcagaa tgatatgcgg gtggccctca tcaacatgga atttattgac gcctaatttg
    20341 atgtcgacgt taaccgtagc gttctggctc atagcgcggt tgctccctta atgccatctt
    20401 tcagcaagtt gatcagccca attttcttcg cgtgtcccat gatcattttg cgtaccggct
    20461 ggtttggttt gccgtcaatg gtgaacatct cgttcatgat gtcggctacc attcgtgggt
    20521 attggctaaa caggcgcggg ttttccatca gcgccgggat cttgcgaaaa tgctgcatat
    20581 cacgcatgac gcagctttgt tccagctcac gtttgtattg cgccagactg ttggcggaga
    20641 aatccgcgcg ttctttggcg gcgatcaccg ttgtggcggc agcctgagcc gatgcaatgg
    20701 ctaaatccat gccgcggacc gtaaaaccca aattcaggca gaagcctgcg gcgtcaccaa
    20761 cgatcatcac gccctcgtta accagttgcg gcaccattgc cagaccgcct tccggcacca
    20821 tatgcgcgga atattcaagc agtttgccgc cgctaatcag cgggcgaatg gcggggtgtt
    20881 gtttaaaatc ttccagcatt tgcggcacgc ttttttgcgc atgggcgata tcacccaatc
    20941 cacaaaccag ccccaaggat atggaatcct tgttggtata gagaaatccc ccgcccatca
    21001 ggccgtcaga aggggaaccg gcaaacagcc aggcggcacc ttcctcgccc gtaatattaa
    21061 agcgatcgtt gatctgttct ggtgtgaggc caataacctc tttaacacca acagcgtaat
    21121 gatgcggatc ggaagcggga accattccca gcgagcggcc aagcatcgag ttaacgccat
    21181 cagccagaat caccacattc gcttcgagaa tatcatcccc agcctgcacg ccagtgacct
    21241 tgtttccttc acgaaccagc gcgtcgacgc gaacgcctgg gataaactgc gcgcccgcct
    21301 gctcggcttg ttccatcaac cacgggtcca gacgattacg caatacggta taagatgcgt
    21361 gttgaggaac atctggttgc tcgcggtgaa aatcgagggt aacggcgctt tcttcggtta
    21421 agaaggagat tttctcgcgt gtgaccttgc gttctaccgg cgctgatact gcaaagcctg
    21481 gaatgattgc ttcaagtgtg tgggcataaa gacgcccgcc ggtcatgttt ttacatccgg
    21541 cactgtcgcc gcgttctatc accaggacat ccagcccggc tcgtgccatg accagtgcgg
    21601 caacgctacc agcaacgccc gcaccgacca caatggcatc aaatttgtca tccgacatgc
    21661 gttatgctcc cttcctgctg cgtttacagc gggagatgtg gttgaaaggg gaattagcgg
    21721 ctcaactggc taatcagggc agggacgact ttgtagatat cgcccaccag accgtagtcg
    21781 gcatagttga agattggcgc atttttatct ttattgatgg cgacaatcac ttttacgccg
    21841 ttgccgccaa ccatatgttg gatctgcccg gagatcccca gcgtcaggta gagatcggat
    21901 ttcagcaaca cgccggagac accgatataa cgttcacgct ccatccagtt ctcgccttcg
    21961 gcaattggac gtgaacagcc gacttcagca ttcagcaccg ccgccagttc gtggaccatt
    22021 ttcaggtcat cctgcgccgc cagaccacga ccgacgccaa ccacacgttt tgctttgctc
    22081 aggtccacgc tgcttgcggc tttcgcacgg cgttcgcgac agagaatttc atgacgcgga
    22141 gcaacataag gtaccgtttc tgtcgggcac tgatgagagg tatcactggt gcacggttcc
    22201 tgaacaccgg gtgcaagggt aatgatcgcc agcgggctgt tgatcttttc ctgagcgaac
    22261 gctaacccgc catacatccg gtgttcggcg caaatgtgac catcgacaat atccaccgcc
    22321 gtggcatcgt tcaccagcgc cgcattcagt tgcacactta accgtgctgc cagagcttta
    22381 ccacgtttcg tcgcggccaa cagcaacata gcggggtgtt tatctttcag cagggcagca
    22441 atgctttcgg cgtaattttc agtgcgttgc agcgcgtcgt tttgctcaag aacataaata
    22501 cattttggac cataaggcat aactgcctgc gcctggtcgg tattttgtac aatggcatac
    22561 acctgttggc cccattgctg agcgccgcca aacagttcag cataacgttc aggattatcg
    22621 ctaaagaccc agacgctgtt taattgactc atcgtttctt ctccgtaggc ttcagttcag
    22681 ggctttcttc agatgttccg ccagctcggc aatggcctcc ggcgaatcgt tatcgagaat
    22741 gatgtgctta cgttctgttt gcggcggtac gcgaatgccg accagttcag caagtggcgc
    22801 gctctggctc cagtcaatat cacttgcctg ccactgattt accggttttt tacccgcgcc
    22861 gagaatggct ttcatcgaag gaatgcgtgg cacgttaata tcggaggtga cgcagagcac
    22921 ggctggaaca gagagttcaa taacttcaac atcatcttca agcgtgcgtt caatcaccag
    22981 tgtattgccc tgacgctgaa tagcactcac tgcattaatc accggaagtt gcagaatttc
    23041 tccgaccagc aagccaacct gctgggcata aaggtcgccg gaaccttcac caaagatcag
    23101 taaatcgaag ccgatctttt caattgctgc cgccagcgct tttgcggtat cgagaggcag
    23161 tgcatgttca agttgcgcat cctgcaccaa atacaggctg tgcggcccgc gggatagcac
    23221 gtctttgcgc actttcgagt tctgcaacaa tgagccgcca acggtcagcg cggctatctc
    23281 atcgtcatct gttgcgagct ggcttgcagc ttcaatggca ttgagatcga actggctgat
    23341 tttggcgtcg gcattgtcga aattcagggt gtattctgga gtgacaacaa tgtcctgttc
    23401 ttcaggcacc agcttaaagc aggttattat tttcatgatt tctcctgtga gtcgcatacg
    23461 gaaccatctc cgatggaagg taaaaatggc ctgaaaatag tgtgcgatag cggtgcgggg
    23521 ggggggggcg acggagcaag ctgttttcct tgccaacaat cttgcaaaaa aaatgcgatt
    23581 gctaaaacaa aagtgcaatt tgcggcaata tagttattcc atgaccgtac aatcttgcat
    23641 tcagtgtaaa taatcttgtt tagctctcgc taaacgcggt tgtataaccg caatacacgc
    23701 cgtattttat ccggcatata ttgtttttgc ttgctcatgg aatagggagg ttgtcatgta
    23761 tcaacgctgt tttgataatg ccagtgaaac gctgtttgtc gccggtaaaa cgccacggct
    23821 ttcacgtttt gcatttagcg atgatccaaa atgggagtct ggacatcacg ttcatgacaa
    23881 tgaaaccgag ctgatttacg tcaagaaagg ggttgcaagg tttaccatcg attcttcgtt
    23941 atatgtcgca catgcggatg acattgtggt gatagaacgc ggcaggctgc atgcggtggc
    24001 ctctgacgtt aacgatccgg caacgacgtg tacctgtgcg ctgtacggct ttcagtttca
    24061 gggggctgag gaaaatcagt tactgcaacc gcattcttgt ccggtaattg ccgcggggca
    24121 gggaaaagaa gtcataaaaa ccttatttaa tgagctaagt gtgattttgc cgcaaagtaa
    24181 aaatagccaa acatcttcgt tatgggatgc attagcctat acattagca
//
LOCUS       DAJTRC010000058        24091 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.058, whole genome shotgun sequence.
ACCESSION   DAJTRC010000058 DAJTRC010000000
VERSION     DAJTRC010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 24091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.058"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..180
                     /gene="yjbD"
                     /locus_tag="OO357_002493"
     CDS             <1..180
                     /gene="yjbD"
                     /locus_tag="OO357_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3811 domain-containing protein"
                     /protein_id="HCR5887406.1"
                     /translation="RVLTNSETNSIKKEYIDKLMVEREAEAKKARQLKKKQAYKPDPE
                     ASFSWSANTSTRGRR"
     gene            complement(313..1185)
                     /gene="rluF"
                     /locus_tag="OO357_002494"
     CDS             complement(313..1185)
                     /gene="rluF"
                     /locus_tag="OO357_002494"
                     /EC_number="5.4.99.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(2604) synthase RluF"
                     /protein_id="HCR5887407.1"
                     /translation="MLPDSSVRLNKYISESGICSRREADRYIEQGNVFLNGKRATIGD
                     QVKPGDIVKVNGQLIEPREAEDLVLIALNKPVGIVSTTEDGERDNIVDFVNHSKRVFP
                     IGRLDKDSQGLIFLTNHGDLVNKILRAGNDHEKEYLVTVDKPITDEFIRGMGAGVPIL
                     GTVTKKCKVKKEAPFVFRITLVQGLNRQIRRMCEHFGYEVKKLERTRIMNVSLSGIPL
                     GEWRDLTDDELIDLFKLIENSSSEAKPKAKAKPKTAGIKRPVVKMEKTAEKGGRPASN
                     GKRFTSPGRKKKGR"
     gene            1533..2480
                     /locus_tag="OO357_002495"
     CDS             1533..2480
                     /locus_tag="OO357_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HCR5887408.1"
                     /translation="MENSDDIRLIVKIAQLYYEQDMTQAQIARELGIYRTTISRLLKR
                     GRDQGMVTIAINYDYNENLWLEQQLKQKFGLKDVVVVSGNDEDEETQLAMMGLHGAQL
                     LDRLLEPGDIVGFSWGRAVSALVENLPQAGQSRQLICVPIIGGPSGKLESRYHVNTLT
                     YSAAAKLKGESHLADFPALLDNPLIRNGIMQSQHFKTISAYWDNLDIALVGIGSPAIR
                     DGANWHAFYGGEESDDLNARQVAGDICSRFFDIHGAMVETNMSEKTLSIEMNKLKQAR
                     YSIGIAMSEEKYSGIIGALRGKYINCLVTNSSTAELLLK"
     gene            2550..3356
                     /locus_tag="OO357_002496"
     CDS             2550..3356
                     /locus_tag="OO357_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5887409.1"
                     /translation="MQTWLNLQDKIIIVTGGASGIGLAIVEELLAQGANVQMVDIHGG
                     DGQYEGHKGYQFWPTDISSTKEVNHTVAEIIQRFGRIDGLVNNAGVNFPRLLVDEKAP
                     AGQYELNEAAFEKMVNINQKGVFLMSQAVARQMVKQHDGVIVNVSSESGLEGSEGQSC
                     YAATKAALNSFTRSWSKELGKHGIRVVGIAPGILEKTGLRTPEYEEALAWTRNITVEQ
                     LREGYTKNAIPIGRAGRLAEVADFVCYLLSERASYITGVTTNIAGGKTRG"
     gene            3366..3773
                     /locus_tag="OO357_002497"
     CDS             3366..3773
                     /locus_tag="OO357_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sorbose transporter subunit IIA"
                     /protein_id="HCR5887410.1"
                     /translation="MVNAIFCAHGKLACAMLESVQMVYGNANVEAVAFVPGENAGDIA
                     VKLEKLVSIHNQDEWLIAVDLQCGSPWNAAAMLAMRNPRLRVISGLSLPLALELVDNQ
                     DSMNVDELCEHLTQIAKQTCVVWKQLATTEEDF"
     gene            3773..4267
                     /locus_tag="OO357_002498"
     CDS             3773..4267
                     /locus_tag="OO357_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
                     /protein_id="HCR5887411.1"
                     /translation="MNITLARIDDRLIHGQVTTVWSKVANAQRIIICNDEVYNDEVRR
                     TLLRQAAPPGMKVNVVNIEKAVAVYHNPQYQDETVFYLFTRPQDALAMVRQGVKIGTL
                     NIGGMAWRPGKKQLTKAVSLDDDDINAFHELNNLGVILDLRVVASDPSINIIDKINEQ
                     LIAN"
     gene            4333..5130
                     /locus_tag="OO357_002499"
     CDS             4333..5130
                     /locus_tag="OO357_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HCR5887412.1"
                     /translation="MEISTLQIIAIFLFSCIAGMGSVLDEFQTHRPLIACTVIGLILG
                     DLKTGIILGGTLELIALCWMNVGAAQSPDSALASIISAILVIVGQQSIATGIAIALPV
                     AAAGQVLTVFARTITVVFQHAADKAAEEARFRTLDILHVSALGVQALRVAIPALIVSL
                     FVSADMVSNMLNAIPEFVTRGLQIAGGFIVVVGYAMVLRMMGVKYLMPFFFLGFLAGG
                     YLDLSLLAFGGVGVIMALVYIQLNPQWCKAEPQLQATASTALDQLDD"
     gene            5141..5929
                     /locus_tag="OO357_002500"
     CDS             5141..5929
                     /locus_tag="OO357_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HCR5887413.1"
                     /translation="MEQRKITRSDLVSMFLRSNLQQASFNFERIHGLGFCYDMIPAIK
                     RLYPLKEDQVAALRLHLVFFNTTPAVCGPVIGAEIDDGTINGIKVGLMGPLAGVGDPL
                     VWGTLRPITAALGASLALSGNILGPLLFFFIFNAVSLAMKWYGLQLGFRKGVNIVSDM
                     GGNVLQKLTEGASILGLFVMGVLVTKWTSINVPLVVSQTHAADGSTVTMTVQNILDQL
                     CPGLLALGLTLLMVRLLNKKINPVWLIFALFGLGIIGNALGFLS"
     gene            5981..7210
                     /gene="sorE"
                     /locus_tag="OO357_002501"
     CDS             5981..7210
                     /gene="sorE"
                     /locus_tag="OO357_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-sorbose 1-phosphate reductase"
                     /protein_id="HCR5887414.1"
                     /translation="MKTTALRLYGKRDLRLETFDLPEMQEDEILATVVTDSLCLSSWK
                     EANLGENHKKVPDDVTTNPIIIGHEFCGDILAVGKKWQHKFQPGQRYVIQANLQLPDR
                     PDCPGYSFPWVDGEATHVVIPNEVMEQYCLLAYDGETYFEGSLVEPLSCVIGAFNANY
                     HLQEGSYNHTMGIRPQGHTLILGGTGPMGLLAIDYALHGPVNPSLLVITDTDNDKLSY
                     ARKHYPSEPQTLIHYLNATDAAFDTLMALSGGHGFDDIFVFVPNEGLVTLASSLLATD
                     GCLNFFAGPQDKHFSAPINFYDVHYAFTHYVGTSGGNTDDMRAAVKLIEEKKVQAAKV
                     VTHILGLNAAGETTLELPAVGGGKKLVYTGKYLPLTSLTQIQDQALAAILARHQGIWS
                     GEVEQYLLAHAEAISHD"
     gene            7592..8281
                     /gene="pepE"
                     /locus_tag="OO357_002502"
     CDS             7592..8281
                     /gene="pepE"
                     /locus_tag="OO357_002502"
                     /EC_number="3.4.13.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepE"
                     /protein_id="HCR5887415.1"
                     /translation="MELLLLSNSTLPGKAWLEHALPLIAEQLQGRRSAVFIPFAGVTQ
                     TWDDYTEKTAAVLAPLGVSVTGIHSVVDPVAAIENAEIVIVGGGNTFQLLKQCRERGL
                     LAPITDVVKRGALYIGWSAGANLACPTIRTTNDMPIVDPQGFDALNLFPLQINPHFTN
                     ALPEGHKGETREQRIRELLVVAPELTIIGLPEGNWITVSKGHATLGGPNTTYVFKAGE
                     EAVPLEAGHRF"
     gene            complement(8372..10003)
                     /locus_tag="OO357_002503"
     CDS             complement(8372..10003)
                     /locus_tag="OO357_002503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na/Pi cotransporter family protein"
                     /protein_id="HCR5887416.1"
                     /translation="MLTLLHLLSAVALLVWGTHIVRTGVMRVFGARLRTVLSRSVEKK
                     PLAFCAGIGVTALVQSSNAPTMLVTSFVAQDLVALAPALVIVLGADVGTALMARILTF
                     DLSWLSPLLIFIGVIFFLGRKQSRAGQLGRVGIGLGLILLALELIVQAVTPITQANGV
                     QVIFASLTGDILLDALIGAMFAIISYSSLAAVLLTATLTAAGIISFPVALCLVIGANL
                     GSGLLAMLNNSAANAAARRVALGSLLFKLVGSLIILPFVHLLAETMGKLSLPKAELVI
                     YFHVFYNLVRCLVMLPFVDPMARFCKTIIRDEPELDTQLRPKHLDVSALDTPTLALAN
                     AARETLRIGDAMEQMMEGLNKVMHGEPRQEKELRKLADDINVLYTAIKLYLARMPKEE
                     LAEEESRRWAEIIEMSLNLEQASDIVERMGSEIADKSLAARRAFSLDGLKELDALYEQ
                     LLSNLKLAMSVFFSGDVTSARRLRRSKHRFRILNRRYSHAHVDRLHQQNVQSIETSSL
                     HLGLLGDMQRLNSLFCSVAYSVLEQPDEDEGRDEY"
     gene            complement(10223..13906)
                     /gene="metH"
                     /locus_tag="OO357_002504"
     CDS             complement(10223..13906)
                     /gene="metH"
                     /locus_tag="OO357_002504"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709803.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HCR5887417.1"
                     /translation="MSSKVEQLRAQLNERILVLDGGMGTMIQSYRLNEADFRGERFAD
                     WPCDLKGNNDLLVLSKPEVIAAIHNAYFEAGADIIETNTFNSTTIAMADYQMESLSAE
                     INFAAAKLARACADEWTARTPEKPRYVAGVLGPTNRTASISPDVNDPAFRNITFDQLA
                     AAYRESTKALVEGGADLILIETVFDTLNAKAAVFAVKTEFEALGVELPIMISGTITDA
                     SGRTLSGQTTEAFYNSLRHAEALTFGLNCALGPDELRQYVQELSRIAECYVTAHPNAG
                     LPNAFGEYDLDADTMAKQIREWAQAGFLNIVGGCCGTTPQHIAAMSRAVEGLAPRKLP
                     EIPVACRLSGLEPLNIGDDSLFVNVGERTNVTGSAKFKRLIKEEKYSEALDVARQQVE
                     NGAQIIDINMDEGMLDAEAAMVRFLNLIAGEPDIARVPIMIDSSKWDVIEKGLKCIQG
                     KGIVNSISMKEGVDAFIHHAKLLRRYGAAVVVMAFDEQGQADTRARKIEICRRAYKIL
                     TEEVGFPPEDIIFDPNIFAVATGIEEHNNYAQDFIGACEDIKRELPHALISGGVSNVS
                     FSFRGNDPVREAIHAVFLYYAIRNGMDMGIVNAGQLAIYDDLPAELRDAVEDVILNRR
                     DDGTERLLELAEKYRGSKADDTANAQQAEWRSWDVNKRLEYSLVKGITEFIEQDTEEA
                     RQQATRPIEVIEGPLMDGMNVVGDLFGEGKMFLPQVVKSARVMKQAVAYLEPFIEASK
                     EQGKTNGKMVIATVKGDVHDIGKNIVGVVLQCNNYEIVDLGVMVPAEKILRIAKEVNA
                     DLIGLSGLITPSLDEMVNVAKEMERQGFAIPLLIGGATTSKAHTAVKIEQNYSGPTVY
                     VQNASRTVGVVAALLSDTQRDDFVARTCKEYETVRIQHGRKKPRTPPVTLEAARDNDF
                     AFDWQAYTPPVAHRLGVQEVEASIETLRNYIDWTPFFMTWSLAGKYPRILEDEVVGVE
                     AQRLFKDANDMLDKLSAEKTLNPRGVVGLFPANRVGDDIEIYRDETRTHVINVSHHLR
                     QQTEKTGFANYCLADFVAPKLSGKADYIGAFAVTGGLEEDALADAFEAQHDDYNKIMV
                     KALADRLAEAFAEYLHERVRKVYWGYAPNENLSNEELIRENYQGIRPAPGYPACPEHT
                     EKATIWELLEVEKHTGMKLTESFAMWPGASVSGWYFSHPDSKYYAVAQIQRDQVEDHA
                     RRKGMSVSDVERWLAPNLGYDAD"
     gene            14106..14930
                     /gene="iclR"
                     /locus_tag="OO357_002505"
     CDS             14106..14930
                     /gene="iclR"
                     /locus_tag="OO357_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate bypass operon transcriptional
                     repressor IclR"
                     /protein_id="HCR5887418.1"
                     /translation="MVAPIPAKRGRKPAVATAPATGQVQSLTRGLKLLEWIAESNGSV
                     ALTELAQQAGLPNSTTHRLLTTMQQQGFVRQVGELGHWAIGAHAFMVGSSFLQSRNLL
                     AIVHPILRNLMEESGETVNMAVLDQSDHEAIIIDQVQCTHLMRMSAPIGGKLPMHASG
                     AGKAFLAQLSEEQVTKLLHRKGLHAYTHATLVSPVHLKEDLAQTRKRGYSFDDEEHAL
                     GLRCLAACIFDEHREPFAAISISGPISRITDDRVTEFGAMVIKAAKEVTLAYGGMR"
     gene            15247..17431
                     /gene="espL4"
                     /locus_tag="OO357_002506"
                     /pseudo
     CDS             15247..17431
                     /gene="espL4"
                     /locus_tag="OO357_002506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418441.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector EspL4"
     gene            complement(17400..19137)
                     /gene="aceK"
                     /locus_tag="OO357_002507"
                     /pseudo
     CDS             complement(17400..19137)
                     /gene="aceK"
                     /locus_tag="OO357_002507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional isocitrate dehydrogenase
                     kinase/phosphatase"
     gene            complement(19321..20625)
                     /gene="aceA"
                     /locus_tag="OO357_002508"
     CDS             complement(19321..20625)
                     /gene="aceA"
                     /locus_tag="OO357_002508"
                     /EC_number="4.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HCR5887419.1"
                     /translation="MKTRTQQIEELQKEWTQPRWEGITRPYSAEDVVKLRGSVNPECT
                     LAQLGAAKMWRLLHGESKKGYINNLGALTGGQALQQAKAGIEAVYLSGWQVAADANLA
                     ASMYPDQSLYPANSVPAVVERINNTFRRADQIQWSVGIEPGDPRYVDYFLPIVADAEA
                     GFGGVLNAFELMKAMIEAGAAAVHFEDQLASVKKCGHMGGKVLVPTQEAIQKLVAARL
                     AADVTGVPTLLVARTDADAADLITSDCDPYDSEFITGERTSEGFFRTHAGIEQAISRG
                     LAYAPYADLVWCETSTPDLELARRFAQAIHAKYPGKLLAYNCSPSFNWQKNLDDKTIA
                     SFQQQLSDMGYKFQFITLAGIHSMWFNMFDLANAYAQGEGMKHYVEKVQQPEFAAAKD
                     GYTFVSHQQEVGTGYFDKVTTIIQGGTSSVTALTGSTEESQF"
     gene            complement(20655..22256)
                     /gene="aceB"
                     /locus_tag="OO357_002509"
                     /pseudo
     CDS             complement(20655..22256)
                     /gene="aceB"
                     /locus_tag="OO357_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418438.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
     gene            complement(22525..23454)
                     /gene="metA"
                     /locus_tag="OO357_002510"
     CDS             complement(22525..23454)
                     /gene="metA"
                     /locus_tag="OO357_002510"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005132837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HCR5887420.1"
                     /translation="MPIRVPDELPAVNFLREENVFVMTTSRASGQEIRPLKVLILNLM
                     PKKIETENQFLRLLSNSPLQVDIQLLRIDSRESRNTPAEHLNNFYCNFEDIQEQNFDG
                     LIVTGAPLGLVEFNDVAYWPQIKQVLEWSKDHVTSTLFVCWAVQAALNILYGIPKQTR
                     TDKLSGVYEHHILHPHALLTRGFDDSFLAPHSRYADFPAALIRDYTDLEILAETEEGD
                     AYLFASKDKRIAFVTGHPEYDAQTLAQEYLRDVEAGLDPDVPYNYFPHNDPQNKPRAS
                     WRSHGNLLFTNWLNYYVYQITPYDLRHMNPTLD"
     gene            23611..24054
                     /locus_tag="OO357_002511"
     CDS             23611..24054
                     /locus_tag="OO357_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HCR5887421.1"
                     /translation="MVISIRRSRHEEGEELVAIWCRSVDATHDFLSAEYRAELEELVR
                     SFLPEAPLWVAVNEREQPVGFMLLSGQHMDALFIDPDVCGCGVGRMLVEHALSMAPEL
                     TTNVNEQNEQAVGFYKKVGFKVTGRSEVDDLGKPYPLLNLAYVGE"
BASE COUNT         5945 a         6146 c         6110 g         5890 t
ORIGIN      
        1 cgcgtactga ccaactccga aaccaacagc attaagaaag agtacatcga taaattaatg
       61 gtcgagcgtg aggctgaagc gaaaaaagcc cgccaattga agaaaaagca ggcttataaa
      121 ccggatcctg aagcgtcatt tagctggtca gcaaatacgt cgacgcgcgg caggcgctaa
      181 tgaacaatca acgcctgaga ttttcactcg tctgtgtgga cacaatcttc ccctcaccct
      241 aaccctctcc ccgaaggggc gaggggactg tccgagctct ttttggacgt tttcatcagt
      301 ctgaggcgtt aatcagcgtc ccttcttttt acgccccggc gaggtaaaac gcttaccgtt
      361 ggacgccggg cgaccgcctt tttccgccgt tttttccatc ttaacgactg gacgtttgat
      421 gcccgctgtt ttcggtttgg ccttcgcttt aggttttgcc tcggaagagg aattttcgat
      481 gagcttaaag aggtcgatta actcatcgtc ggttaaatcg cgccactccc ccagcggaat
      541 gccgcttaag ctgacgttca taatgcgcgt acgttccagc tttttcactt catagccgaa
      601 atgctcgcac atgcgccgga tctgacggtt cagcccctgc actagggtaa tgcggaagac
      661 aaacggcgct tcttttttga ctttgcactt tttcgtcacc gtcccgagga tcggcacccc
      721 cgcccccata ccacgaataa attcatcggt aatcggttta tcgaccgtca ccagatactc
      781 tttctcatga tcgttacccg cacggaggat cttattcacc agatcgccgt gattggtgag
      841 gaaaatcagc ccctgggagt ctttatccag gcggccaatc gggaacacgc gtttgctgtg
      901 gttaacgaaa tcgacaatgt tatcgcgctc gccatcttcg gtggtgctga caataccgac
      961 gggcttgttc agggcgataa gtaccaaatc ttcggcttcc cgaggctcaa tcaactgacc
     1021 gtttactttc acaatgtcgc cgggtttcac ctgatcgcca atggtggctc gcttgccatt
     1081 aaggaacaca ttgccttgct cgatatagcg atccgcttcg cggcgtgagc aaattccgct
     1141 ttcgctgatg tatttattta aacggactga tgagtcgggc agcataaatt ctcctgtaaa
     1201 acgcggaata taccgtacct cttagcgggg aaaaacaggg ttatgcggca acgtcgaaat
     1261 taaatgtgat ctgccacgcc ttttatcaca aaagtgtctc cggaagtacg atgcaaattt
     1321 gcctggcaaa ataaggataa tcaacatatt acgaatcgct gcacacctgt gctgatgact
     1381 actacgcaga gaaacaaatg tgcaagagaa agcgctattt ctccccatgc aaagagtgaa
     1441 aaatcgcttt gaaaggccct catgatgggg ttatatcaga tcaagcggtg agaaagcgta
     1501 tttgcacaaa tgtgcagaac caggaggcgg caatggaaaa cagtgacgat atccgtttga
     1561 ttgtgaagat tgcccaactc tattacgaac aggatatgac gcaggcgcaa atcgcgcgcg
     1621 aactgggtat ttaccgcacc accatcagcc gcttgcttaa acgaggccgc gatcagggaa
     1681 tggtcaccat cgccatcaac tatgactaca acgaaaatct ctggctggag cagcaactga
     1741 agcaaaagtt tggcctgaaa gacgttgtgg tggtgtcggg aaatgatgag gatgaagaga
     1801 ctcaactggc gatgatgggg ttacacggcg cgcaactgct ggatcgcttg ctggagcctg
     1861 gcgatattgt cggtttttcc tggggtcgcg cggtgagcgc actggttgaa aacttgccgc
     1921 aggcggggca atcgcggcag ttaatctgcg taccaattat tggcggaccg tccggtaaac
     1981 tcgaaagccg ctatcatgta aacacattaa cctacagcgc ggcagcgaag ctgaaagggg
     2041 aatcgcatct cgcggatttt ccggctctgc tggataaccc attaattcgt aatgggatca
     2101 tgcagtctca gcactttaaa accatctctg cctactggga taatctggat atcgccctgg
     2161 tgggaattgg ctcaccggcc attcgcgacg gcgctaactg gcatgcgttt tatggtggtg
     2221 aagagagtga cgacctgaat gcccgccagg ttgctggcga tatttgctcg cgcttttttg
     2281 atattcacgg cgcaatggtt gaaacgaata tgagcgaaaa aacgctctct atcgaaatga
     2341 ataaattaaa gcaggcgcga tattccattg gcattgccat gagtgaagaa aaatacagcg
     2401 gaattattgg tgcactgcgt ggaaaatata ttaattgtct ggtaacgaat agcagcacag
     2461 ctgaactatt actgaaataa aaatcttccc gatgtttacc gtaataaaaa ttacggcggg
     2521 tccctattat ctaaaatcag gagtctgtta tgcaaacgtg gttaaatttg caggataaaa
     2581 tcattattgt caccggcggc gcatccggta ttggtctggc gattgtagag gaattattag
     2641 cacaaggcgc gaatgtacag atggtcgata ttcacggtgg cgatggtcaa tatgaaggtc
     2701 ataaaggcta tcagttttgg ccgaccgata tttctagcac aaaagaggta aatcatacgg
     2761 tagcggaaat tatccagcgt tttggtcgca tcgacggtct ggtcaataac gccggggtca
     2821 atttcccgcg tctgctggtc gatgagaaag cgcctgccgg gcagtatgaa ctcaacgaag
     2881 ctgcattcga aaaaatggtc aatatcaacc agaaaggtgt ttttctgatg tcgcaggcgg
     2941 tggcgcgaca gatggtcaaa caacatgatg gcgtgattgt gaatgtttcc tcagaaagtg
     3001 ggctggaagg ctcagaagga caaagctgtt acgccgctac caaagccgcg ctcaatagct
     3061 tcacgcgctc ctggtcgaaa gagctgggta aacacggtat ccgtgtggtc ggtatcgcgc
     3121 ccgggattct ggaaaaaacc gggctacgca cgccggaata tgaagaggcg ctggcgtgga
     3181 cgcgcaatat caccgtcgag cagctgcgtg aaggctatac caaaaacgcc attcctattg
     3241 ggcgcgccgg aagattagca gaagtagctg attttgtttg ttatctgttg tctgaacgcg
     3301 ccagctacat caccggagta accactaaca ttgcgggcgg caaaacgcgc ggctaaggag
     3361 gctttatggt caacgccatt ttttgtgccc acggcaaatt agcttgcgcc atgctggaat
     3421 cggttcagat ggtttacggc aacgccaatg ttgaagccgt tgcatttgtg cccggtgaaa
     3481 acgccggaga cattgcggta aagctggaaa agttagtaag cattcacaat caagatgagt
     3541 ggctgattgc cgtggattta cagtgcggca gcccgtggaa tgcggcagca atgcttgcga
     3601 tgcgcaatcc tcgtttgcgg gtaatcagcg gcctttctct gccgctggcg ctcgaactgg
     3661 ttgataacca ggacagcatg aatgttgatg aactgtgtga acacctgacg caaatcgcaa
     3721 agcaaacctg cgttgtctgg aaacaactgg caacgacaga ggaggatttc tgatgaacat
     3781 tacgctcgcc cgcattgatg accgtctgat ccacggccag gtcactaccg tctggtcgaa
     3841 agtggctaac gcccagcgca ttattatctg caatgacgaa gtttataacg atgaagtgcg
     3901 ccggacatta ttgcgtcagg ctgcgccacc gggaatgaag gttaacgtgg tcaatattga
     3961 aaaagccgtc gcggtttatc acaacccgca atatcaggac gaaaccgttt tttatctttt
     4021 cactcgccca caggatgcct tagcgatggt tcgccagggg gtaaaaatag gcaccctgaa
     4081 tattggcggc atggcctggc ggcccggtaa aaaacagtta accaaagcgg tttcattaga
     4141 tgatgatgat attaacgcat ttcatgagtt aaataatctt ggtgtcatac tggatttacg
     4201 cgtcgtagcg tcagatccct caatcaatat tatcgacaaa ataaacgaac agttaatcgc
     4261 aaattaaata atagcgcctg tgtacatatg ctttaacagg cagggatgac gacacttaaa
     4321 aggtgacaaa tcatggaaat aagtacccta caaatcattg ctatatttct tttttcctgt
     4381 attgccggaa tgggcagcgt gctggatgaa tttcagactc atcgcccgtt aattgcctgt
     4441 acggtgattg gtttaattct cggtgattta aaaaccggaa taatactcgg cggtacgctg
     4501 gaattgatag ctctctgctg gatgaacgtc ggcgcggcgc aatctccgga ttctgcactc
     4561 gccagcataa tctccgccat tctggttatc gttggtcagc aaagcatcgc caccggaatc
     4621 gccatcgcgt tgcctgtggc tgcggcaggc caggtgctga cggtgtttgc ccgtaccatc
     4681 accgtagtgt tccagcacgc ggcggataaa gcagcagaag aggcgcggtt tcgcactctc
     4741 gatattctgc atgtctccgc gcttggcgtg caggcgctgc gcgtcgccat tccggcactg
     4801 attgtctcac tgttcgtcag cgccgatatg gtcagcaata tgttaaacgc cattcccgaa
     4861 tttgtgaccc gaggactaca gattgctggc ggttttatcg tggtggtcgg ttacgccatg
     4921 gtgctacgta tgatgggcgt gaaatatttg atgcctttct ttttcctcgg tttcctcgca
     4981 ggtggctacc tcgatctcag cctgctggcg ttcggtggcg tcggcgtgat catggccctg
     5041 gtctacatcc agctaaatcc acagtggtgt aaagctgaac ctcaactcca ggccaccgcc
     5101 tccaccgccc ttgaccaact tgacgattaa cggagccatc atggaacaga gaaaaattac
     5161 acgcagcgat ctggtgagca tgtttctgcg ctccaacctg caacaggcgt cctttaactt
     5221 tgaacgtatt cacgggctgg gcttttgcta cgacatgatc cccgccatca agcgacttta
     5281 cccattaaaa gaggatcagg ttgcggcgct caggctacac ctggtgttct tcaataccac
     5341 gccagccgta tgtggcccgg tcatcggcgc ggaaattgat gacggtacca tcaacggcat
     5401 caaagtcggt ctgatgggac cattggcagg agttggcgat ccactggtct ggggaacgct
     5461 gcgcccgatt accgccgcgc tcggcgcatc tctggcactt tcgggcaaca ttctcggccc
     5521 gctgctgttc ttctttattt tcaacgcggt gagtctggcg atgaagtggt atggcctaca
     5581 gctcggcttt cgcaaagggg tgaatatcgt cagcgatatg ggcgggaatg tgctgcaaaa
     5641 actcaccgaa ggcgcgtcga ttctcggact gtttgtgatg ggcgtgctgg tgaccaaatg
     5701 gacgtcaatc aacgtaccgt tggtggtttc acaaacgcat gccgccgatg gctccaccgt
     5761 caccatgacc gtgcagaaca ttctcgacca actttgccct ggtttgctgg cgctcggtct
     5821 gacgctacta atggttcgtc tgctcaacaa aaaaattaac ccggtatggc tgattttcgc
     5881 cctgtttggc ttagggatta tcggcaatgc tctgggcttc ctgtcctgat tcttcgcccc
     5941 ggcacgactg ccgaggccat cgctcaacat gaggtggttt atgaaaacaa cagctctgcg
     6001 tctttatggt aaacgtgatt tacgcctgga aacctttgac cttcctgaaa tgcaggagga
     6061 tgaaatcctc gcaacggtgg tcactgacag cctgtgcctc tcttcctgga aagaggccaa
     6121 tctgggtgaa aaccataaaa aagtacccga cgatgtgacg accaacccaa tcatcatcgg
     6181 ccacgaattt tgcggcgata ttctggccgt gggtaaaaag tggcagcaca aattccagcc
     6241 gggtcagcgt tatgtgattc aggccaacct gcaactcccc gaccgcccgg attgccccgg
     6301 ctactccttc ccgtgggtag acggcgaagc gacgcatgtg gttattccca acgaggtcat
     6361 ggaacaatat tgcctgctgg catacgacgg cgaaacctat tttgaaggct cactggttga
     6421 accgctttcc tgcgtgattg gcgcgttcaa cgccaactat catcttcagg aaggtagtta
     6481 taaccacacg atggggattc gcccgcaagg gcacacgctg atcctcggcg gcaccggacc
     6541 aatgggactg ttggcgattg attatgcgct gcatggacca gttaacccgt cgctgctcgt
     6601 cattaccgat accgacaacg acaaattgag ttatgcgcgc aagcactatc catcagaacc
     6661 gcaaacactg attcattatc tcaatgccac cgatgcagca tttgatacgt taatggcgct
     6721 gagtggcggt cacggcttcg atgatatttt cgtctttgtg cctaatgaag gactggtgac
     6781 tctcgcctct tccttgctgg cgacagatgg ttgcctgaat ttcttcgccg gaccgcagga
     6841 taaacatttc agcgcgccaa ttaatttcta cgatgtgcat tatgcattta cccactacgt
     6901 gggcacgtca ggcggcaata ccgacgacat gcgcgcggcg gtcaaactga tcgaagagaa
     6961 aaaagtgcag gccgcaaaag tggtaacaca tattcttggg ctgaatgccg cgggcgaaac
     7021 cacgcttgaa ttgcctgccg tcggcggcgg caaaaagctg gtgtataccg ggaaatacct
     7081 gccgctgacg tcactcacgc agattcagga tcaagcactg gcggcgattc tggcgcgtca
     7141 tcaggggatc tggtccggtg aggtggagca atacctgctc gcccatgcag aggcaatttc
     7201 ccatgattaa tcgcgctact taactctgca tgtcgctggc tggtcgcccc agcaacttcg
     7261 cgaccggatg tgcggctgat tgctgaagca gcacaatttg cacgagaagg ataaaaaata
     7321 tcagcgcctt aaggggcgct caaattggtg gcaaagtaca cattgtttat gccggatgcg
     7381 gaatgaagcc ttattcggtc tacataattt tgaaaatcca atatattgca gttatctggt
     7441 aggccaggta agcgtagcgc atcaggcaat gctgtgtttg tcatcagtct cagcgcccgg
     7501 tgcaatacgt gacatcgtga gccatgtcag taagtggtta aattcgcagt ggtagcatgc
     7561 cttccgtgat ttcttactaa ccggagaagt aatggaactg cttttattga gtaactcgac
     7621 gctgccgggt aaagcctggc tggaacatgc actgccgcta attgctgaac agttgcaggg
     7681 tcgccgctca gcggtgttta tccctttcgc tggcgtaacg cagacctggg atgattacac
     7741 agagaaaacg gctgcggttc tcgctccgct gggtgtttct gtcaccggta ttcatagcgt
     7801 tgtcgatccc gttgccgcga ttgaaaatgc ggaaattgtg attgtcggcg gcggtaatac
     7861 tttccagttg ctgaaacagt gccgcgagcg cgggctgctg gctccaatta ctgacgtggt
     7921 taaacgtggc gcgctgtata ttggctggag cgcaggcgct aaccttgctt gcccaaccat
     7981 tcgtaccacc aacgatatgc cgattgtcga tccgcaaggt ttcgatgcgc taaatctgtt
     8041 cccgttgcaa atcaacccgc acttcaccaa cgcgctgccg gaaggccata aaggtgaaac
     8101 ccgtgagcag cgtattcgcg aactgctggt cgtcgcgcca gaactgacga ttattggtct
     8161 gccggaaggt aactggatca cagtgagtaa aggtcacgct acgctgggtg gcccgaacac
     8221 cacttatgtg tttaaggctg gtgaagaagc ggttccgctg gaagctggtc accgttttta
     8281 agtgaattgc cggatgcatg atccggcatc ttgttcatag tgcctgatgc gacgcttgac
     8341 gcgtcttatc aggcctacag gattcacaat attaatactc gtcccgtcct tcatcttcat
     8401 ccggctgttc cagcacactg taagccaccg aacaaaacag cgagttcagg cgctgcatat
     8461 ctcccagtaa gcctaaatgt agcgaactgg tttcaatgct ctgcacgttt tgctgatgca
     8521 ggcgatcgac gtgggcgtgg gaatagcggc gattaagaat gcgaaaacga tgtttgctgc
     8581 gacgcaaacg acgagcgctg gtgacatcgc cagagaagaa caccgacatt gccagcttta
     8641 aattactgag caattgctca tagagcgcat ccagttcctt caacccatca agcgaaaatg
     8701 cccgccgtgc tgccagcgat ttatcggcaa tttcgctgcc catgcgctcg acgatatcgg
     8761 aggcctgttc aaggttgagc gacatttcga tgatctccgc ccagcggcgc gactcttcct
     8821 ccgccaactc ttcttttggc atccgcgcca gatacagctt aatggcggtg tagagaacgt
     8881 tgatatcatc tgccagctta cgcagctctt tctcctgccg tggctcgccg tgcatcactt
     8941 tattcagccc ttccatcatc tgttccatgg cgtcgccaat gcgcagggtt tcgcgcgcgg
     9001 cgttcgccag agcaagcgtg ggcgtgtcca gcgcgctgac atccagatgt ttaggccgta
     9061 gctgggtatc cagttccggt tcatcgcgaa taatcgtttt gcaaaaccgt gccatcgggt
     9121 caacaaatgg cagcatgacc aggcaacgta caaggttgta gaagacgtgg aaatagatca
     9181 ccagttccgc ttttggcaat gacagcttcc ccattgtctc tgccagcaaa tggacaaacg
     9241 gcaggataat caggctaccc accagcttaa acagcagact acccagcgcg acacggcggg
     9301 ctgcggcatt ggcggcactg ttgttgagca tcgccagcag gccggaaccg aggttagctc
     9361 caatcaccag acagagcgcc acggggaagg agataatgcc tgcagcggtc agagtcgcgg
     9421 tcagcagtac agcagcaagg ctggagtagc tgataatggc gaacatcgcg ccaatcagcg
     9481 catccagcag aatatcgccg gtcagcgagg caaagatcac ctgaacgccg tttgcctggg
     9541 tgatcggcgt tacggcctgc acaatcaact ccagcgccag caaaatcagc ccaagaccaa
     9601 taccgacgcg gcccagttgc ccggcgcgtg actgtttgcg tccgaggaaa aaaatcacgc
     9661 cgataaaaat aagtaacggt gacagccagg ataagtcgaa ggtgagaata cgcgccatta
     9721 gcgccgtccc gacatctgcc cccagcacaa tgaccagagc cggtgcgagg gctaccagat
     9781 cctgagcgac gaacgaggtc accagcatgg tgggggcatt actgctctgt accagtgcgg
     9841 taacgccgat ccccgcgcaa aaggcgagcg gctttttttc gacgctccgg ctaaggacag
     9901 tacgcaaacg cgcgccgaag acgcgcatta cgccggttcg aacaatatga gtcccccaga
     9961 ccagcagggc gacggcagaa agcaggtgaa gcagcgttaa cacgagttca gattctcctt
    10021 atcgttatat gttcctgaag caattgaatg accaaaggac cggaaacacg ctgactggca
    10081 tctttactgt gccttttgac attcgttttt ccggctaaga ttattccagc ctgtttccag
    10141 tataagtgtt tcatattaat aaagagacag ggcactcatt gagcgccctg tttgttgtaa
    10201 ggaaaagtga cagatttgtg aatcagtccg cgtcataccc cagattcggt gccagccagc
    10261 gctcaacatc ggagacgctc atacctttac ggcgggcatg gtcttcaacc tgatcgcgct
    10321 gaatttgcgc caccgcgtag tacttgctgt ccggatgact gaaataccaa cccgaaaccg
    10381 atgcaccagg ccacatcgcg aaagattctg tgagtttcat gccagtatgt ttttccactt
    10441 ccagcagctc ccagatggtg gctttttccg tatgttccgg gcaggccgga tagcccggtg
    10501 ccggacggat gccctggtag ttttcgcgga tcagttcttc gttgctgagg ttctcgttcg
    10561 gcgcatagcc ccagtagact ttacgcacac gctcatggag atactccgca aaggcttcgg
    10621 ctaaacggtc ggcaagcgct ttcaccatga ttttgttgta atcatcgtgc tgcgcttcaa
    10681 aggcatcagc cagtgcgtcc tcttccagcc cgccagtcac ggcaaatgcg ccgatgtaat
    10741 ctgctttacc agaaagcttc ggcgcaacga agtcagcgag acagtagtta gcgaatcccg
    10801 ttttttcggt ctgttgacgc agatggtggc tgacgttgat cacatgggtg cgcgtttcgt
    10861 cacggtagat ttcaatgtca tcgcccacac ggtttgccgg gaacaggccc accacgccac
    10921 gcggattcag cgttttctcg gcgcttaatt tatccagcat gtcgttggcg tctttaaaca
    10981 gccgctgcgc ctcaacgccc accacttcat cttccagaat gcgaggatac ttcccggcca
    11041 gcgaccaggt cataaagaac ggtgtccagt cgatgtaatt acgcagcgtt tcgatgctgg
    11101 cttccacttc ctgcacgccg agacggtgcg ccaccggcgg cgtgtaagcc tgccagtcaa
    11161 atgcgaaatc gttatcgcgc gccgcttcca gcgtgaccgg tggtgtgcgt ggtttcttgc
    11221 gcccgtgctg aatacgcacg gtttcgtact ccttgcaggt acgagcgaca aaatcatcac
    11281 gctgagtatc ggaaagcagc gccgctacca caccaacggt gcgcgaggca ttctgcacat
    11341 acaccgtagg gccgctgtag ttctgctcga ttttcaccgc cgtgtgcgct tttgaggtgg
    11401 tcgcgccgcc aatcagtaac ggaatggcga agccctgacg ctccatctct ttcgccacgt
    11461 taaccatctc gtccagcgac ggcgtgataa gccccgaaag gccaatcaaa tcagcattca
    11521 cttctttggc gatacggaga attttttccg caggcaccat aacgccgaga tcgacaattt
    11581 cgtagttgtt acattgcagc accacaccaa cgatattttt accgatgtcg tggacatcgc
    11641 ctttcacggt ggcgatcacc atcttgccgt tggttttacc ctgctctttg ctggcttcaa
    11701 taaacggttc gagatacgcc actgcctgtt tcatgacgcg cgccgatttg accacctgtg
    11761 gcaggaacat tttgccttcg ccaaacagat cgccgaccac gttcatgccg tccatcaacg
    11821 ggccttcaat cacttcaatc gggcgcgtag cctgctggcg ggcttcttca gtatcctgct
    11881 cgataaactc ggttatgcct ttgaccagcg agtattccag acgtttattc acgtcccacg
    11941 agcgccactc cgcttgctgg gcgttagcag tgtcgtccgc tttactgccg cgatatttct
    12001 cggcaagctc cagtaaacgc tcagtgccat cgtcacgacg attaagaatc acatcttcca
    12061 ccgcgtcgcg cagttcagcg ggtaggtcgt cgtaaatcgc cagttgcccg gcgttgacga
    12121 tccccatatc catgccattg cgaatagcgt agtagaggaa cactgcgtga atggcttcgc
    12181 gcaccggatc gttgccacgg aatgagaaag aaacgttaga tacgccgccg gaaatcagcg
    12241 cgtgcggcag ttcgcgtttg atgtcttcac acgcgccgat aaagtcctgc gcgtagttgt
    12301 tgtgctcttc aataccggtg gcgaccgcga agatgttcgg gtcgaagatg atatcttctg
    12361 gcgggaagcc aacctcttcg gtgaggattt tgtacgcccg acggcaaatc tcgattttcc
    12421 gtgcgcgagt atcggcctgt ccctgttcgt caaaggccat caccaccact gccgcaccgt
    12481 agcgacgcaa caatttcgcg tgatggataa aggcgtcgac gccctctttc atcgagatag
    12541 agttaacaat gcctttgccc tggatacact tcagaccttt ttcaatgaca tcccattttg
    12601 aggagtcgat cataatcggc acgcgagcga tatccggttc accggcaatc agattgagaa
    12661 aacgcaccat cgccgcttcg gcatcgagca tcccttcatc catgttgata tcgataatct
    12721 gcgcgccgtt ttccacctgc tgacgcgcca catccagcgc ctcgctgtat ttctcttctt
    12781 tgatcaggcg cttgaactta gcagagccgg tgacgttggt gcgttcaccc acgttcacaa
    12841 acaggctatc gtcgccaatg ttcagcggct ccaggccaga caaacggcag gctacgggaa
    12901 tttccggcag tttgcgcggc gctaatcctt ctactgcacg actcatcgct gcaatatgtt
    12961 gcggcgtggt cccgcagcaa ccaccgacga tattgagaaa gcccgcttgc gcccattcac
    13021 gtatctgttt tgccatcgtg tcggcgtcga gatcgtattc accaaaggcg ttaggcagcc
    13081 cggcgttcgg gtgggcggtg acgtagcatt ccgcaatccg tgatagttcc tgcacgtact
    13141 ggcgcagttc atcgggcccc agcgcacagt tcaggccaaa ggtcagcgct tcggcgtggc
    13201 gcaatgagtt gtaaaatgct tcggtggtct gcccggagag cgtgcgcccg gaggcgtcgg
    13261 tgatggtgcc ggagatcata atcggcagct caacgcccag cgcttcaaac tccgttttca
    13321 ccgcaaagac cgccgctttg gcgttaaggg tgtcgaaaac ggtttcaatc aggatcagat
    13381 ccgcaccacc ttccaccagc gctttggtgg attcgcgata ggccgccgcc agctggtcaa
    13441 aagtgatgtt gcggaatgcc ggatcgttga cgtccggaga aatagacgcc gtgcggttgg
    13501 ttgggccaag aacaccggca acgtagcgcg gtttctccgg cgtgcgcgcg gtccactcgt
    13561 cagcacaggc gcgcgccagt ttcgccgccg caaagttgat ttccgccgac agggattcca
    13621 tctggtaatc cgccatcgca atggtcgtgg agttgaaggt gttggtttcg atgatatccg
    13681 cgcccgcttc aaagtaggcg ttgtggatag cggcgatcac ttccggttta ctgagtacca
    13741 gcaggtcgtt gttgcctttg aggtcgcatg gccagtcggc aaagcgttca ccacgaaaat
    13801 cggcttcgtt cagtcgatag ctctggatca tggtgcccat accgccgtcc aacaccagaa
    13861 tacgttcatt taactgcgca cgcagttgtt ccactttgct gctcacactt gctcccgaca
    13921 cgcttaaccc agatttaata aaaattcaac aaaccatact ggaataaacg catctgtggt
    13981 aaaagcgacc accacgcaac atgagatttg ttcaacatta actcatcgga tcagttcagc
    14041 aactattgca ttagctaaca ataaaaatga aaataatttc cacgatacag aaaaaggagg
    14101 ctgtcatggt cgcacccatt cccgcgaaac gcggcagaaa acccgccgtt gccaccgcac
    14161 cagcgactgg acaggttcag tctttaacgc gtggcctgaa attactggag tggattgccg
    14221 aatccaatgg cagcgtggca ctcacagagc tggcgcaaca agccgggtta cccaattcca
    14281 cgacccaccg cctgctaacc acgatgcaac agcagggttt cgtgcgtcag gtcggcgaac
    14341 tgggacactg ggcaatcggc gcacatgcct ttatggtcgg cagcagcttt ctccagagcc
    14401 gtaatttgtt agcaattgtt caccctatcc tgcgcaatct aatggaagag tctggcgaaa
    14461 cggtcaatat ggcggtgctt gatcaaagcg atcacgaagc gattattatc gaccaggtac
    14521 agtgtaccca cctgatgcga atgtccgcgc ctatcggcgg taaattgccg atgcacgctt
    14581 ccggtgcggg taaagccttt ttagcccaac tgagcgaaga acaggtgacg aagctgctgc
    14641 accgcaaagg gttacatgcc tatacccacg caactctggt gtctcctgtg catttaaaag
    14701 aagatctcgc ccaaacgcgc aaacggggtt attcatttga cgatgaggaa catgcgctgg
    14761 ggctacgttg ccttgcagcg tgtattttcg atgagcaccg cgaaccgttt gccgcaattt
    14821 ctatttccgg accgatttca cgtattaccg atgatcgcgt gaccgagttt ggcgcgatgg
    14881 tgattaaagc ggcgaaggaa gtgacgctgg cgtacggtgg aatgcgctga ctttttctgg
    14941 cgggcagagg caatattctg cccatcatac ctgagtggca atagaataag ggtgtctgtt
    15001 aatcgcattg acgccaaaat aacttaatgt catacacttc acacactttt attaactcag
    15061 cattattttt aaacatcaaa ccacttaatt attaacaaaa tcgcaagatc tcattccacc
    15121 gaaactttta cggctgcatt atgagattga gcgatttatg atattttttt agttgtaatc
    15181 atattaaatt ttttcactgt tctgatagtt aaaattcaag acatcaataa acaatgagat
    15241 atttaaatga ttactcgtat tcctcgtagt tctttctctg caaatattaa taatacagcc
    15301 cagacaaatg aacaccaaac cctgagtgaa ttgttttata aagaactcga ggataaattt
    15361 tctggcaagg agctggcgac gcctctatta aaaagcttct cagagaactg tagacaaaat
    15421 gttcgtcata tctttagcaa caaggatttt gtcattaaat tttccacgtc cgtcttacaa
    15481 gctgataaga aagaaattac gatgattaat aaaaacgaaa acacgacact cactcaaacc
    15541 attgctacaa tatttgaaga atacctaatg gaaattttac ctcaacgctc agacactctt
    15601 gataaacaag aattaaacca aaaatcagat agaaaagaaa aagaattccc aagaattaaa
    15661 cttaatggtc aatgttattt cccggggcgc cccaaaaccg tatagtatac cgacacattg
    15721 ctgcacaata tattaatgat atttatcaga atgttgatta caaaccccat caagatgatt
    15781 actcttcagc tgaaaaattt ctcactcact tcaacaaaaa atgcaaaaac cagactttgg
    15841 cgttgatttc cagccgtcct gaggggcgtt gcgttgctgc ctgcggtgat ttcgggctag
    15901 ttatgaaagc atattttgac aagatggaat caaatggcat cagtgttatg gcagccatat
    15961 tactggtgga taaccatgct ttgacggtcc ggctaagaat aaagaacaca actgaaggat
    16021 gtacccatta cgtggtttcg gtttatgatc ctaatgtaac taacgataaa ataagaatta
    16081 tgagcgaaag caaagaggat attaaacact attctctgat ggattttatg aatgtagatt
    16141 atagcctcct gaaatggtca aatgatcatg ttattaacca atctgttgca ataattccag
    16201 cacttccgaa agaacagcta ttgatgttaa aaggaactgt ggatgaaata acccctccat
    16261 tatcaccagc aacgatgaat ttgctaatgg caattggtca gaatcaccaa cttacgcaac
    16321 tgatgattca gctccagaaa atgccagaac tacatagaac agaaatgttg actgcctata
    16381 atagtattaa cctccccggt ttatatttgg ctataaatta tggcaatgcg gatatcgttg
    16441 agactatttt caattcattg tcagaaacag gatatgaagg gttactctcg aaaaaaaatc
    16501 tcatgcatat tctggaggca aaagataaaa atggtttttc tggattattt ttagcgatat
    16561 cacgtaagga taaaaatgtt gtaacctcga ttctgaacgt cttacctaaa ctggccgcaa
    16621 cacatcattt agataatgaa caggtgtata aattcctgag tgccaaaaat agaacgtcca
    16681 gccatgtttt ataccatgtt atggcgaatg gtgatgccga catgctgaaa atttttttgg
    16741 tcgcgttacc tttgttaatt cgcacatgtc atttgactaa agaacaggta ctcgatctcc
    16801 tgaaggcaaa ggatttttat ggttgcccaa gactatacct ggcgatgcaa aatggacata
    16861 gcgatatcgt gaaagttatt ctcgaagcat tgccctgtct ggcccaggaa attaacattt
    16921 cagcttccga tattgtagat cttctgaccg ctaaaagtct tgcgcgcgac acgggtttgt
    16981 ttatggccat gcagcgcgga catatgaacg ttattaatac tatttttaac gcattaccca
    17041 ctctttttaa tacgtttaaa ttcgataaaa aaatatgaag cccctcctcc tggcaaataa
    17101 ttctaatgaa tatcccggtt tgttttcagc gatacagcat aaacaacaaa acgttgtaga
    17161 gacggtttat cttgctttat ctgaccatgc acgcctgttt ggatttaccg ctgaagatat
    17221 tatggatttt tggcaacaca aagcgccaca aaaatactct gcctttgagt tggcttttga
    17281 atttggtcac cgggttattg ctgaattaat ccttaataca ttaaataaga tggctgaaag
    17341 ctttggcttt acggataacc ctcgatacat tgcggagaaa aattatatgg aagctttact
    17401 caaaaaagca tctccccata ccgtacgcta aatctttgcc tgcgccgata cgcataaaca
    17461 tcttccacat gcccttcacg tatgcggttt tgtagtgcgc gccagtaatc agcgcggaac
    17521 aggtcggcgt gcatctcttc aaacagcgga ccaatacgcg ggtcggcgca tagccagtgg
    17581 cgaaactctt ccgggaaaac atcgcccggc gagacgctgt accacggttc gctggcaagt
    17641 tcgtcttccg gatagcgcgg cggcgggatg tcgcggaaat tcacttccgt catgtagcaa
    17701 atttcatcgt aatcataaaa aaccacacgc ccgtgacggg tgacaccgaa gtttttaaac
    17761 agcatgtcgc cagggaaaat gttagcagcg gcaagctggc gaatagcgtt accgtattct
    17821 tcaatggcgt cgcgcaactg ctgaccttcc acttgttcca gccagatatt gagcggcacc
    17881 atccgccgct cgattataga gatggcgaat tacaatctgc tcaccaagat cggtgatttt
    17941 ttccgctgct tcctggagca gtaattccat taataccggg gaaatatgcc gcttctccag
    18001 cacaaagttt tcaaactcct gggtgtccgc cattcggccc acacggtcgt gctctttcac
    18061 cagttgatag caggcacgaa cgtgagcggc agacatctct ttctgcggcg cgaacttgtc
    18121 tttgatgact ttgaataccc gatcaaagcc cggcagcgta aacaccaaca tcaccattcc
    18181 acgaataccc ggcgcttcaa tgaactgctc attacagccc tgtagataaa cgagatattc
    18241 gcggtagctt tcggttttgg cgtgcttctg gcagccgata gccatataca attcagcagt
    18301 ggttttacct ggcagaattt cccgtagcca ctcgaccagc gctgcgggca gcggtgcata
    18361 aaccataaaa taagaacgcg caaagccaaa aacaatgctc gcttcggcgg tcgtcgtcag
    18421 gcaggtatca ataaataact cgccgtcgtc cgtctggtgg atcggcagta agaagggcaa
    18481 cgtgccggaa ggcgtgatca gtttgcccac cagccaggcg gctttattgc ggtaaaacag
    18541 ttcgttcgcc acctgtaaat gactttccgc gaggttgtct gtccccagcg tttccgtcag
    18601 atggcgaatg atgtaatgaa tgtcacggct tttattctgc cagcgcaggc gcagcggcag
    18661 gtcgctgata acgcgcatca gtagagattc ccagccgtga tcggggtgaa agtctttcgc
    18721 cagcgggcgg ggaatggtac gaaagcggcg ctctggctga gagctaaaga taaaaagccg
    18781 ctcgggagta agcgagcggt ggtcaaataa ccgacagtac acggagttaa aaaagctctc
    18841 cgcaatctcg aagcgcgggt aatccggcaa cagccgcgtg taatgctctt taacgcgcag
    18901 taaaaatgcc gcgtccgtac tttggccgtt agtaatgcag cgcagttgct ccacgaccag
    18961 accaacgtga tgatcgtaaa gatggatacg gtttttcatc gcctgctgga cagcatgcca
    19021 gtcggcctgt tcgaaacgct gctgcgcccc ggaagtcact tcgaggaatc gaccatactg
    19081 agcgtcgaag ccttgcaaaa tggtttgagc aatcagtaat tccaggccac gcggcattta
    19141 gcgccctcat caggagcaga gaattgccgg atgcggcgta aacgccttat ccagcctacg
    19201 ttcggcaacg gttgtaggca tgataagacg cgcaagcgtc gcatcaggca tcgtgcaccg
    19261 attgccggat gcggcgtaaa tgccttatcc ggcctacagt cagcaacgtt tgtaggttgc
    19321 ttagaactgc gattcttcag tggagccagt cagcgcggtg actgaagacg tgccgccctg
    19381 aataatagtc gtcactttat cgaagtaacc tgtacccact tcctgctggt gagatacgaa
    19441 ggtatagcca tctttcgcgg cggcaaattc cggctgctgc actttctcaa catagtgctt
    19501 cataccttcg ccctgcgcat aagcgtttgc caggtcgaac atgttaaacc acatgctgtg
    19561 gatacctgct agggtgatga actggaactt gtagcccata tccgacagct gctgctggaa
    19621 gctggcaata gttttgtcgt cgaggttttt ctgccagttg aacgacggcg agcagttata
    19681 agccagcagt ttgcccggat atttcgcgtg gatagcttgt gcaaagcgac gcgccagttc
    19741 cagatccggc gtggaggttt cacaccagac caggtcagca tatggcgcat acgccaggcc
    19801 acggctgatc gcttgctcaa tgcccgcatg agtacggaag aagccttcac tggtacgctc
    19861 gccggtaata aattcgctgt catacgggtc gcaatctgag gtgatcagat ccgccgcatc
    19921 agcatcggta cgtgcaacca gcagggttgg aacgcccgtc acgtcagctg ccagacgcgc
    19981 cgcgaccagt ttctgaatag cttcctgagt tggcactaaa actttgccgc ccatgtgacc
    20041 gcatttcttc actgacgcca gctgatcttc gaagtgaact gccgcagcac cggcttcaat
    20101 catcgctttc atcagttcaa aggcattcag gacaccgcca aaaccggctt ccgcatcggc
    20161 aacgatcggc aggaagtaat cgacatagcg cggatcgccc ggctcaatgc ccacggacca
    20221 ttggatctga tcggcacgac ggaaggtgtt gttgatccgc tccaccacag ctggcaccga
    20281 gtttgccgga tagagcgact gatccggata catgctggcc gccaggttag cgtccgccgc
    20341 tacctgccat cccgacagat agactgcttc aatacccgct ttcgcctgtt gcagcgcctg
    20401 accgccagtc agtgcaccga ggttgttgat gtagcctttt ttcgactcac cgtgcagcag
    20461 acgccacatt ttcgctgcgc ccagttgcgc cagcgtgcat tcaggattga ctgaaccgcg
    20521 taatttcacc acatcttccg cactgtatgg gcgagtaatg ccttcccaac gcggttgagt
    20581 ccactctttc tgtaattctt caatttgttg tgtacgggtt ttcatgtgca gatgctccat
    20641 agttatgtgg tggtttacgc taacaggcgg tagcctggca gggtcaggaa atcaattaac
    20701 tcatcggaag tggtgatctg ttccatcaag cgtgcggcat cgtcaaaacg gccctgggag
    20761 aaacgttctt cgcccagttc gctggcaatg actttcatct cttcgcccag catctggcgg
    20821 aacaaggctt tggtcaccgg tttgccattg ctcaacgttt tttgatgatg gatccactgc
    20881 cagatcgagg tacgggaaat ttcagccgtc gccgcatctt ccatcaggcc ataaatcggc
    20941 acgcagccgt tgccggagat ccacgcttcg atgtactgca cagccacgcg aatgttagcg
    21001 cgcatacctt cttcggtgcg ttcaccatca caaggtgcca gcagctgatc ggcagtaatc
    21061 ggcgcgtctt gttcgcgcaa tacttcaagc tgatttttac gggagccgag aatgtcgttg
    21121 aataccgcca ttgccgtgtc cgcaaggcct gggtgagcga tccatgtgcc atcgtgaccg
    21181 ttattggctt ccagcgcttt atccgctttt actttgttga gcacctggtt attacgctct
    21241 tcatctttgc tcggaataaa cgcggccatg ccgcccatcg caaaagcacc gcgtttatgg
    21301 caggttttaa tcagcaggcg tgagtaagca ttcaggaatg gtttatccat cgtcacggcc
    21361 tgtctgtctg gcaggacacg atcgggatag tttttcaacg ttttgatata gctgaagatg
    21421 taatcccagc gaccgcagtt cagaccaaca atatggtcgc gcagcgcgtg aaggatttcg
    21481 tccatctgga acacggcggg cagcgtttca atcagcaacg tcgccttgat ggtgccgcgc
    21541 ggcagattaa agcgatcttc tgcatagctg aagacttcgc tccaccaggc cgcttcctgc
    21601 taggactggg ttttcggcag atagaaatag ggaccgctgc cctttgccaa cagtgcctga
    21661 tagttgtgga agaaatagag cgcaaaatca aacaggctac cggggattgc ctcaccacgc
    21721 caggtgacat gtttttccgg caagtgcaga ccgcgtaccc gacaaatcaa aaccgctgga
    21781 ttgggcttga gctgataaat tttgcctgct tcattggtat agctgatggt accgttaacc
    21841 gcatcgcgca ggttaatttg cccgtcgatc actttgttcc agtctggtgc cagtgaatct
    21901 tcgaaatcgg ccataaagac tttcacattg gcgttcagtg cgttgatcac catcttgcgc
    21961 tctaccgggc cagttatctc tacgcggcgg tcttctaagt ccgcaggaat cccgcgaatt
    22021 ttccaatcag catcgcgaat ggaagccgtt tccgaaataa aatcaggcaa cgttccgtta
    22081 tcaatatctt gctgctgctg aatgcgcgct gccagaagtt tattgcgttg tggcgtaaaa
    22141 tgcgtcacca gctcagtcag aaattctacc gcttcggcag taagaatttg cttctcctgc
    22201 tcgccatacg gccttgtgaa agccagttca tcggttgttg ttgcctgttc agtcatcgtg
    22261 cagctcctcg tcatggatcc gaaaacttcc cctctgtgaa cgaaggaacg ttgtgcgctt
    22321 ttcgttcagc acaactaaga ctactcattt aaaatgcaaa atcaaaaaca atttccattt
    22381 taattaaaaa tacttgataa cttattgata acaatatgaa taaaatttat tcacttgctg
    22441 aggtgcgttt cggaaataaa aaaggcaccc gaaggtgcct gaggtaaggt gctgaatcgc
    22501 ttaacgatcg actatcacag aagattaatc cagcgttgga ttcatgtgcc gtagatcgta
    22561 tggcgtgatc tgatagacgt aatagttgag ccagttggta aacagcaaat taccgtgact
    22621 acgccagctc gctcgcggtt tgttttgcgg atcgttgtgc gggaaatagt tatacggtac
    22681 atccgggtct agtccggctt ccacatcgcg taaatattcc tgcgccagcg tttgcgcatc
    22741 atattcggga tggcccgtca caaaggcaat gcgcttatct ttgctggcaa acagatatgc
    22801 atccccttct tccgtctctg ccagaatttc cagatcggtg taatcacgaa tcaacgctgc
    22861 cggaaagtca gcatagcgcg aatgcggtgc caggaatgaa tcatcaaagc cacgcgtcag
    22921 aagcgcatga ggatggagaa tatgatgctc gtaaacgcca gagagtttgt cggtgcgagt
    22981 ttgcttagga atgccgtaga ggatattgag cgcggcctgt accgcccagc agacaaacag
    23041 cgtcgaggtg acgtgatctt tcgaccactc cagcacctgt ttgatctgcg gccagtaagc
    23101 gacatcatta aactccacca ggcccagcgg cgcaccggtt acaatcagac cgtcaaagtt
    23161 ctgttcctga atatcttcaa agttacagta gaagttgttc agatgctctg cgggcgtgtt
    23221 gcgcgattca cgggaatcga tgcgcaacag ctgaatatcg acctgcaaag gtgagtttga
    23281 aagcaggcgc agaaactgat tttcagtttc aatcttcttc ggcatcaggt taaggatcag
    23341 aaccttaagt ggacgaattt cctgaccaga cgcacgagaa gttgtcatca caaagacgtt
    23401 ttcttcacgc aagaaattga cggcgggtag ctcgtccggc acacgaatcg gcataacctg
    23461 attacctcac tacatacgct tatacgttta gacatccaga tagctgaaga taaccagaaa
    23521 atttgccaat gtcgagcctg catgttgaag gtgagaaagt ttcaaggaaa aggctacgtt
    23581 agaatataag aatgacgaaa aggagagaaa atggttatta gtattcgccg ctcacggcat
    23641 gaggaagggg aggaactcgt tgcgatttgg tgtcgatctg tcgatgccac ccacgatttt
    23701 ttatcagcag agtatcgggc cgagctggaa gagctggtgc gttcctttct gccggaagca
    23761 ccgttgtggg tcgcggttaa tgaacgggag cagccggttg ggtttatgtt gttaagtggg
    23821 cagcatatgg atgcgctgtt tatcgatcct gatgtgtgcg gctgcggcgt gggtcggatg
    23881 ctggtggagc atgcgctctc gatggcaccg gaactgacaa ccaacgttaa tgagcaaaat
    23941 gagcaggcgg ttgggttcta taagaaggtg ggttttaagg ttacgggacg ctctgaggtg
    24001 gacgatttgg ggaaaccgta tccgttgctg aatctggcgt atgtggggga ataagccgcc
    24061 tctgccgtgc ctgcccaagc tggagaagac g
//
LOCUS       DAJTRC010000059        23302 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.059, whole genome shotgun sequence.
ACCESSION   DAJTRC010000059 DAJTRC010000000
VERSION     DAJTRC010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 23302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.059"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..803
                     /gene="tesB"
                     /locus_tag="OO357_002512"
     CDS             <1..803
                     /gene="tesB"
                     /locus_tag="OO357_002512"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706346.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HCR5887422.1"
                     /translation="LFRGQSEDLGLRQVFGGQVVGQALYAAKETVPEERLVHSFHSYF
                     LRPGDSKKPIIYDVETLRDGNSFSARRVAAIQNGKPIFYMTASFQAPEAGFEHQKTMP
                     SAPAPDGLPSETQIAQSLAHLLPPVLKDKFICDRPLEVRPVEFHNPLKGHVAEPHRQV
                     WIRANGSVPDDLRVHQYLLGYASDLNFLPVALQPHCIGFLEPGIQIATIDHSMWFHRP
                     FNLNEWLLYSVESTSASSARGFVRGEFYTQDGVLVASTVQEGVMRNHN"
     gene            complement(852..2138)
                     /gene="amtB"
                     /locus_tag="OO357_002513"
     CDS             complement(852..2138)
                     /gene="amtB"
                     /locus_tag="OO357_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter AmtB"
                     /protein_id="HCR5887423.1"
                     /translation="MKIATIKTGLASLAMLPGLVMAAPAVADKADNAFMMICTALVLF
                     MTIPGIALFYGGLIRGKNVLSMLTQVTVTFALVCILWVVYGYSLAFGEGNNFFGNINW
                     LMLKNIELTAVMGSIYQYIHVAFQGSFACITVGLIVGALAERIRFSAVLIFVVVWLTL
                     SYIPIAHMVWGGGLLASHGALDFAGGTVVHINAAIAGLVGAYLIGKRVGFGKEAFKPH
                     NLPMVFTGTAILYIGWFGFNAGSVGTANEIAALAFVNTVVATAAAILGWIFGEWALRG
                     KPSLLGACSGAIAGLVGVTPACGYIGVGGALIIGVVAGLAGLWGVTMLKRLLRVDDPC
                     DVFGVHGVCGIVGCIMTGIFAASSLGGVGFAEGVTMGHQLLVQLESIAITIVWSGVVA
                     FIGYKLADLTVGLRVPEEQEREGLDVNSHGENAYNA"
     gene            complement(2168..2506)
                     /gene="glnK"
                     /locus_tag="OO357_002514"
     CDS             complement(2168..2506)
                     /gene="glnK"
                     /locus_tag="OO357_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HCR5887424.1"
                     /translation="MKLVTVIIKPFKLEDVREALSSIGIQGLTVTEVKGFGRQKGHAE
                     LYRGAEYSVNFLPKVKIDVAIADDQLDEVIDIVSKAAYTGKIGDGKIFVAELQRVIRI
                     RTGEADEAAL"
     gene            complement(2687..4468)
                     /locus_tag="OO357_002515"
     CDS             complement(2687..4468)
                     /locus_tag="OO357_002515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdB family multidrug efflux ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5887425.1"
                     /translation="MRSFSQLWPTLKRLLAYGSPWRKPLGIAVLMMWVAAAAEVSGPL
                     LISYFIDNMVAKNNLPLKVVAGLAAAYVGLQLFAAGLHYAQSLLFNRAAVGAVQQLRT
                     DVMDAALRQPLSEFDTQPVGQVISRVTNDTEVIRDLYVTVVATVLRSAALVGAMLVAM
                     FSLDWRMALVAIMIFPVVLVVMVIYQRYSTPIVRRVRAYLADINDGFNEIINGMSVIQ
                     QFRQQARFGERMGEASRSHYMARMQTLRLDGFLLRPLLSLFSSLILCGLLMLFGFSAS
                     GTIEVGVLYAFISYLGRLNEPLIELTTQQAMLQQAVVAGERVFELMDGPRQQYGNDDY
                     PLQSGTIEVDNVSFAYRDDNLVLKNINLSVPSRNFVALVGHTGSGKSTLASLLMGYYP
                     LTEGEIHLDGRPLSSLSHSALRQGVAMVQQDPVVLADTFLANVTLGRDISEERVWQAL
                     ETVQLAELARSMSDCIYTPLGEQGNNLSVGQKQLLALARVLVETPQILILDEATASID
                     SGTEQAIQHALAAVREHTTLVVIAHRLSTIIDADTILVLHRGQAVEQGTHQQLLAAQG
                     RYWQMYQLQLAGEELAASVREEESLSA"
     gene            complement(4461..6233)
                     /locus_tag="OO357_002516"
     CDS             complement(4461..6233)
                     /locus_tag="OO357_002516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SmdA family multidrug ABC transporter
                     permease/ATP-binding protein"
                     /protein_id="HCR5887426.1"
                     /translation="MRLFAQLSWYFRREWRRYLGAVALLVIIAMLQLVPPKVVGIVVD
                     GVTEQHFTTGLSLMWIATMVLIAVVVYLLRYVWRVLLFGASYQLAVELREDYYRQLSR
                     QHPEFYLRHRTGDLMARATNDVDRVVFAAGEGVLTLVDSLVMGCAVLIMMSTQISWQL
                     TLFALLPMPVMAIMIKRNGDALHERFKLAQAAFSSLNDRTQESLTSIRMIKAFGLEDR
                     QSALFAADAEDTGKKNMRVARIDARFDPTIYIAIGMANLLAIGGGSWMVVQGSLTLGQ
                     LTSFMMYLGLMIWPMLALAWMFNIVERGSAAYSRIRAMLAEAPVVNDGSEPVPEGRGE
                     LDVNIHQFTYPQTDHPALENVNFALKPGQMLGICGPTGSGKSTLLSLIQRHFDVSEGD
                     IRFHDIPLTKLQLDSWRSRLAVVSQTPFLFSDTVANNIALGCPNATQQEIEHVARLAS
                     VHDDILRLPQGYDTEVGERGVMLSGGQKQRISIARALLVNAEILILDDALSAVDGRTE
                     HQILHNLRQWGQGRTVIISAHRLSALTEASEIIVMQHGHIAQRGNHDELAQQSGWYRD
                     MYRYQQLEAALDDVPEIREEAVDA"
     gene            complement(6263..6721)
                     /gene="decR"
                     /locus_tag="OO357_002517"
     CDS             complement(6263..6721)
                     /gene="decR"
                     /locus_tag="OO357_002517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator DecR"
                     /protein_id="HCR5887427.1"
                     /translation="MLDKIDRKLLALLQQDCTLSLQALAEAVNLTTTPCWKRLKRLED
                     DGILIGKVALLDPEKIGLGLTAFVLIKTQHHSSEWYCRFVTVVTEMPEVLGVWRMAGE
                     YDYLMRVQVADMKRYDEFYKRLVNSVPGLSDVTSSFAMEQIKYTTSLPIE"
     gene            complement(6875..7693)
                     /gene="cof"
                     /locus_tag="OO357_002518"
     CDS             complement(6875..7693)
                     /gene="cof"
                     /locus_tag="OO357_002518"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HMP-PP phosphatase"
                     /protein_id="HCR5887428.1"
                     /translation="MARLVAFDMDGTLLMPDHHLGEKTLSTLARLRERDITLTFATGR
                     HALEMQHILGALSLDAYLITGNGTRVHSLEGELLHRDDLPADVAELVLYQQWDTRASM
                     HIFNDDGWFTGKESPALLQVFVYSGFRYQIIDVKKMPLGSVTKICFCGDHDDLTRLQI
                     QLYEALGERAHLCFSATDCLEVLPVGCNKGAALTVRTQHLGLSLRDCMAFGDAMNDRE
                     MLGSVGSGFIMGNAMPQLRAELPHLPVIGHCRNQAVSHYLTHWLDYPHLPYSPE"
     gene            7793..9485
                     /locus_tag="OO357_002519"
                     /pseudo
     CDS             7793..9485
                     /locus_tag="OO357_002519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308526.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SgrR family transcriptional regulator"
     gene            9550..10245
                     /gene="queC"
                     /locus_tag="OO357_002520"
     CDS             9550..10245
                     /gene="queC"
                     /locus_tag="OO357_002520"
                     /EC_number="6.3.4.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-cyano-7-deazaguanine synthase QueC"
                     /protein_id="HCR5887429.1"
                     /translation="MKRAVVVFSGGQDSTTCLVQALQQYDEVHCVTFDYGQRHRAEID
                     VARELALKLGARAHKVLDVTLLNELAVSSLTRDSIPVPDYEPEADGIPNTFVPGRNIL
                     FLTLAAIYAYQVKAEAVITGVCETDFSGYPDCRDEFVKALNHAVSLGMAKDIRFETPL
                     MWIDKAETWALADYYGKLDLVRNETLTCYNGIKGDGCGHCAACNLRANGLNHYLADKP
                     TVMAAMKQKTGLR"
     gene            complement(10297..10695)
                     /gene="fadM"
                     /locus_tag="OO357_002521"
     CDS             complement(10297..10695)
                     /gene="fadM"
                     /locus_tag="OO357_002521"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain acyl-CoA thioesterase FadM"
                     /protein_id="HCR5887430.1"
                     /translation="MQTQIKVRGYHLDVYQHVNNARYLEFLEEARWDGVENSDSFQWM
                     TAHNIAFVVVNININYRRPAVLSDLLTITSQLQQLNGKSGILNQVITLEPEGQVVADA
                     LITFVCIDLKTQKALALEGELREKLEQMVK"
     gene            complement(10789..11160)
                     /locus_tag="OO357_002522"
     CDS             complement(10789..11160)
                     /locus_tag="OO357_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HCR5887431.1"
                     /translation="MKHGIKALLITLSLACAGMSHSALAAASVAKPTAVETKAEAPAA
                     QSKAAVPAKASDEEGTRVSINNASAEELARAMNGVGLKKAQAIVSYREEYGPFKTVED
                     LKQVPGMGNSLVERNLAVLTL"
     gene            complement(11311..13182)
                     /gene="ppiD"
                     /locus_tag="OO357_002523"
     CDS             complement(11311..13182)
                     /gene="ppiD"
                     /locus_tag="OO357_002523"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HCR5887432.1"
                     /translation="MMDSLRTAANSLVLKIIFGIIIVSFILTGVSGYLIGGGNNYAAK
                     VNDQEISRGQFENAFNSERNRMQQQLGDQYSELAANEGYMKTLRQQVLNRLIDEALLD
                     QYARELKLGISDEQVKQAIFATPAFQVDGKFDNSRYNGILNQMGMTADQYAQALRNQL
                     TTQQLINGVAGTDFMLKGETDELAALVAQQRVVREATIDVNALAAKQPVTEQEIASYY
                     EQNKNNFMTPEQFRVSYIKLVAATMQQPVSDADIQSYYDQHQDQFTQPQRTRYSIIQT
                     KTEDEAKAVLDELNKGGDFAALAKEKSADIISARNGGDMGWLEDATIPDELKNAGLKE
                     KGQLSGVIKSSVGFLIVRLDDIQPAKVKSLDEVRDDIAAKVKHEKALDAYYALQQKVS
                     DAASNDTESLAGAEQAAGVKATQTGWFSKDNLPEELNFKPVADAIFNGGLVGENGASG
                     INSDIITVDGDRAFVLRVSEHKPEAVKPLADVQEQVKALVQHNKAEQQAKVDAEKLLV
                     DLKAGKGAEAMQAAGLKFGEPKTLSRSGRDPISQAAFALPLPAKDKPSYGMATDMQGN
                     VVLLALDEVKQGSMPEDQKKAMVQGITQNNAQIVFEALMSNLRKEAKIKIGDALEQQ"
     gene            complement(13374..13646)
                     /gene="hupB"
                     /locus_tag="OO357_002524"
     CDS             complement(13374..13646)
                     /gene="hupB"
                     /locus_tag="OO357_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016535194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein HU-beta"
                     /protein_id="HCR5887433.1"
                     /translation="MNKSQLIDKIAAGADISKAAAGRALDAIIASVTESLKEGDDVAL
                     VGFGTFAVKERAARTGRNPQTGKEITIAAAKVPSFRAGKALKDAVN"
     gene            complement(13855..16209)
                     /gene="lon"
                     /locus_tag="OO357_002525"
     CDS             complement(13855..16209)
                     /gene="lon"
                     /locus_tag="OO357_002525"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001469947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HCR5887434.1"
                     /translation="MNPERSERIEIPVLPLRDVVVYPHMVIPLFVGREKSIRCLEAAM
                     DHDKKIMLVAQKEASTDEPGVNDLFTVGTVASILQMLKLPDGTVKVLVEGLQRARISA
                     LSDNGEHFSAKAEYLESPTIDEREQEVLVRTAISQFEGYIKLNKKIPPEVLTSLNSID
                     DPARLADTIAAHMPLKLADKQSVLEMSDVNERLEYLMAMMESEIDLLQVEKRIRNRVK
                     KQMEKSQREYYLNEQMKAIQKELGEMDDVPDENEALKRKIDAAKMPKEAKEKAEAELQ
                     KLKMMSPMSAEATVVRGYIDWMVQVPWNARSKVKKDLRQAQEILDTDHYGLERVKDRI
                     LEYLAVQSRVNKIKGPILCLVGPPGVGKTSLGQSIAKATGRKYVRMALGGVRDEAEIR
                     GHRRTYIGSMPGKLIQKMAKVGVKNPLFLLDEIDKMSSDMRGDPASALLEVLDPEQNV
                     AFSDHYLEVDYDLSDVMFVATSNSMNIPAPLLDRMEVIRLSGYTEDEKLNIAKRHLLP
                     KQIERNALKKGELTVDDSAIIGIIRYYTREAGVRGLEREISKLCRKAVKQLLLDKSLK
                     HIEINGDNLHDYLGVQRFDYGRADNENRVGQVIGLAWTEVGGDLLTIETACVPGKGKL
                     TYTGSLGEVMQESIQAALTVVRARAEKLGINPDFYEKRDIHVHVPEGATPKDGPSAGI
                     AMCTALVSCLTGNPVRADVAMTGEITLRGQVLPIGGLKEKLLAAHRGGIKTVLIPFEN
                     KRDLEEIPDNVIADLDIHPVKRIEEVLTLALQNEPSGMQVVTAK"
     gene            complement(16397..17671)
                     /gene="clpX"
                     /locus_tag="OO357_002526"
     CDS             complement(16397..17671)
                     /gene="clpX"
                     /locus_tag="OO357_002526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001396736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATP-binding subunit ClpX"
                     /protein_id="HCR5887435.1"
                     /translation="MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLC
                     NDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNG
                     DTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVEN
                     IIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGT
                     VAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKA
                     KSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALT
                     KQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMED
                     VEKVVIDESVIDGQSKPLLIYGKPEAQQASGE"
     gene            complement(17797..18420)
                     /gene="clpP"
                     /locus_tag="OO357_002527"
     CDS             complement(17797..18420)
                     /gene="clpP"
                     /locus_tag="OO357_002527"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006809880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HCR5887436.1"
                     /translation="MSYSGERDNFAPHMALVPMVIEQTSRGERSFDIYSRLLKERVIF
                     LTGQVEDHMANLIVAQMLFLEAENPEKDIYLYINSPGGVITAGMSIYDTMQFIKPDVS
                     TICMGQAASMGAFLLTAGAKGKRFCLPNSRVMIHQPLGGYQGQATDIEIHAREILKVK
                     GRMNELMALHTGQSLEQIERDTERDRFLSAPEAVEYGLVDSILTHRN"
     gene            complement(18667..19965)
                     /gene="tig"
                     /locus_tag="OO357_002528"
     CDS             complement(18667..19965)
                     /gene="tig"
                     /locus_tag="OO357_002528"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001716227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HCR5887437.1"
                     /translation="MQVSVETTQGLGRRVTITIAADSIETAVKSELVNVAKKVRIDGF
                     RKGKVPMNIVAQRYGASVRQDVLGDLMSRNFIDAIIKEKINPAGAPTYVPGEYKLGED
                     FTYSVEFEVYPEVELQGLEAIEVEKPIVEVTDADVDGMLDTLRKQQATWKEKDGAVEA
                     EDRVTIDFTGSVDGEEFEGGKASDFVLAMGQGRMIPGFEDGIKGHKAGEEFTIDVTFP
                     EEYHAENLKGKAAKFAINLKKVEERELPELTAEFIKRFGVEDGSVEGLRAEVRKNMER
                     ELKSAIRNRVKSQAIEGLVKANDIDVPAALIDSEIDVLRRQAAQRFGGNEKQALELPR
                     ELFEEQAKRRVVVGLLLGEVIRTNELKADEERVKGLIEEMASAYEDPKEVIEFYSKNK
                     ELMDNMRNVALEEQAVEAVLAKAKVTEKETTFNELMNQQA"
     gene            complement(20309..20626)
                     /gene="bolA"
                     /locus_tag="OO357_002529"
     CDS             complement(20309..20626)
                     /gene="bolA"
                     /locus_tag="OO357_002529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414969.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator BolA"
                     /protein_id="HCR5887438.1"
                     /translation="MMIRERIEEKLRAAFQPVFLEVVDESYRHNVPAGSESHFKVVLV
                     SDRFTGERFLNRHRMIYSTLAEELSTTVHALALHTYTIKEWEGLQDTVFASPPCRGAG
                     SIA"
     gene            20931..21509
                     /locus_tag="OO357_002530"
     CDS             20931..21509
                     /locus_tag="OO357_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5887439.1"
                     /translation="MFKKILFPLVALFMLAGCAKPPTTIEVSPTITLPQQDPSLMGVT
                     VSINGADQRTDQALAKVTRDNQIVTLTASRALRFLLQEVLEKQMTARGYMVGPNGPVN
                     LQIIVSQLYADVSQGNVRYNIATKADIAIIATAQNGNKMTKNYRASYNVEGAFQASNK
                     NIADAVNSVLTDTIADMSQDTSIHEFIKQNAR"
     gene            21553..23028
                     /gene="ampG"
                     /locus_tag="OO357_002531"
     CDS             21553..23028
                     /gene="ampG"
                     /locus_tag="OO357_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="muropeptide MFS transporter AmpG"
                     /protein_id="HCR5887440.1"
                     /translation="MSSQYLRIFQQPRSAILLILGFASGLPLALTSGTLQAWMTVENI
                     DLKTIGFFSLVGQAYVFKFLWSPLMDRYTPPFFGRRRGWLLATQILLLVAIAAMGFLE
                     PGTQLRWMAALAVVIAFCSASQDIVFDAWKTDVLPAEERGAGAAISVLGYRLGMLVSG
                     GLALWLADKWLGWQGMYWLMAALLIPCIIATLLAPEPTDTIPVPKTLEQAVVAPLRDF
                     FGRNNAWLILLLIVLYKLGDAFAMSLTTTFLIRGVGFDAGEVGVVNKTLGLLATIVGA
                     LYGGILMQRLSLFRALLIFGILQGASNAGYWLLSITDKHLYSMGAAVFFENLCGGMGT
                     SAFVALLMTLCNKSFSATQFALLSALSAVGRVYVGPVAGWFVEAHGWSTFYLFSVAAA
                     VPGLILLLVCRQTLEYTRVNDNFISRTEYPAGYAFAMWTLAAGVSLLAVWLLLLTMDA
                     LDLTHFSFLPALLEVGVLVALSGVVLGGLLDYLALRKTHLT"
BASE COUNT         5454 a         6450 c         5597 g         5801 t
ORIGIN      
        1 gactctttcg cggccagagt gaagatttag gtttacgcca ggtgtttggc ggccaggtcg
       61 tgggtcaggc cttgtatgct gcaaaagaga ccgtcccgga agagcggctg gtacattcgt
      121 ttcacagcta ctttcttcgc ccaggcgata gtaagaaacc gattatttat gatgtcgaaa
      181 cgctgcgtga cggtaacagc ttcagcgccc gccgggttgc tgctattcaa aacggcaaac
      241 cgatttttta tatgacggcc tctttccagg caccagaagc gggtttcgaa catcaaaaaa
      301 caatgccgtc cgcgccagcg cctgatggcc tgccttcgga aacgcaaatc gcccaatcgc
      361 tggcgcacct gctgccgcca gtgcttaaag ataaattcat ctgcgatcgt ccgctggaag
      421 tccgtccggt ggagtttcat aacccactga aaggtcacgt cgcagaacca catcgtcagg
      481 tgtggatccg cgcaaatggt agcgtgccgg atgacctgcg cgttcatcag tatctgctcg
      541 gttacgcttc tgatcttaac ttcctgccgg tagctctaca gccgcactgc atcggttttc
      601 tcgaaccggg gattcagatt gccaccattg accattccat gtggttccat cgcccgttta
      661 atttgaatga atggctgctg tatagcgtgg agagcacctc ggcgtccagc gcacgcggct
      721 ttgtgcgcgg tgagttttat acccaagacg gcgtgctggt tgcctcgacc gttcaggaag
      781 gggtaatgcg taatcacaat taaaaaaata gccggaggtg aaaaccgtcc ggctattttt
      841 tgcagtgctt gttacgcgtt ataggcattc tcgccgtggc tgttgacgtc cagcccttct
      901 cgctcttgtt cttccggcac acgtaggcca accgtcagat ccgccagttt gtagccaata
      961 aatgccacca caccggacca gacgatcgta atggcgatgc tttccaactg taccagcaac
     1021 tgatggccca tcgtcacacc ttcagcgaag cccacgccgc ccagagaact ggcggcaaaa
     1081 atcccggtca tgatacagcc gacaatgcca caaacgccgt gcacaccgaa gacatcgcag
     1141 ggatcatcca cccgcagcaa gcgtttgagc atggtaacgc cccacaaacc cgccagacca
     1201 gctaccacgc cgataatcaa cgcgccgcca accccaatgt agccgcacgc tggcgtcacg
     1261 ccgaccagac cggcaatcgc gccagaacac gctcccagca gagaaggctt accacgcagc
     1321 gcccattcac cgaagatcca gccaagaatt gccgccgccg ttgcgaccac agtattcaca
     1381 aatgccagtg ccgcgatttc attcgccgtg cccactgacc cggcgttaaa gccaaaccaa
     1441 ccgatataga gaatggcagt cccggtgaag accatcggca ggttgtgcgg tttaaacgcc
     1501 tctttaccga agcctacgcg tttgcctatc agatacgcgc ccaccagacc ggcgattgct
     1561 gcgttaatgt gcaccaccgt gccacccgcg aaatccagcg caccgtgaga agccagcaaa
     1621 ccaccgcccc acaccatatg cgcaatcgga atgtaagaga gcgtcagcca taccaccacg
     1681 aaaatcaaca cagctgagaa gcggattcgt tccgccagcg ccccaactat caagcctacg
     1741 gtaatgcagg caaacgatcc ctgaaacgct acgtggatat actgataaat gctgcccatc
     1801 accgccgtca gttcgatgtt ttttagcatc aaccagttaa tgttgccgaa gaagttgttg
     1861 ccctcaccaa acgccagcga ataaccgtaa accacccaga gaatacagac cagtgcaaac
     1921 gtcaccgtca cctgcgtcag catcgacagc acgtttttgc cgcgaatcaa cccaccgtaa
     1981 aacagggcaa tccccggaat agtcataaac agcaccagcg cagtacaaat catcataaac
     2041 gcattgtcgg ctttatcggc caccgcaggt gcagccatta ccagtcccgg cagcatcgcc
     2101 agtgaagcaa gcccagtttt tatcgtcgct atcttcattt ttcgttcctg ttgctgtgtg
     2161 ccagagatta cagcgccgct tcgtcggctt cgccggtacg aatacgaatg acgcgttgca
     2221 attcagcgac gaagattttg ccgtcgccaa tttttccggt gtaagccgcc ttactgacga
     2281 tatcgatcac ttcatcgagt tggtcatcag caatcgccac atcaattttt acttttggca
     2341 ggaagttgac gctgtattcc gccccccggt acagctcggc atgccctttc tgacgcccga
     2401 aacctttcac ttcggtgacg gtcaggccct gaataccaat ggaagataac gcttcacgaa
     2461 cgtcttccag cttgaatggt ttgattatca cggtcaccag cttcatagat cccctccggt
     2521 cagaattcgg taatggttct gctacacgaa gaaggtattg caagcggtgt gccagaaatg
     2581 aaaaacgaga aagagagcag gaagttaaga agattgaaaa tgaaaacgca ctatgacagt
     2641 gcacagtgcg ttacattttt gcaccaacga tgaaatattg cgctattcag gcgctcaatg
     2701 attcctcttc acgcacgctg gctgccagct cttcgcccgc aagttgcagt tgatacatct
     2761 gccagtagcg tccctgggcc gccagcagtt gctggtgagt gccctgctcc acggcttgcc
     2821 cacgatgaag caccagaatg gtgtcggcat caataatagt tgataagcgg tgagcaatca
     2881 ccaccagcgt ggtatgttca cgcaccgccg ccagagcatg ttgaatcgcc tgttcagtac
     2941 cggagtcaat gctggcggtt gcctcatcaa ggatcaggat ttgcggcgtc tccaccagca
     3001 cgcgcgccag tgccagcagt tgcttttgcc cgactgagag attatttccc tgctcgccca
     3061 gcggcgtata aatacaatca ctcatgctgc gcgccagctc cgccagttgc acggtttcca
     3121 gcgcctgcca gacgcgttct tcggagatat cccgccccag cgtcacatta gcgaggaagg
     3181 tatccgccag caccaccgga tcttgctgca ccattgccac gccctggcgc agcgcgctat
     3241 gacttagcga acttaatgga cgaccatcaa ggtgaatctc accttccgtt agcgggtaat
     3301 agcccatcaa taaactggcg agggtacttt taccactgcc ggtatgcccg accagcgcca
     3361 caaagttacg cgaaggcaca gagagattaa tgttctttag caccagattg tcatcgcgat
     3421 aagcaaatga cacgttatcg acttcgatgg tgccactctg taacgggtaa tcatcattgc
     3481 catattgctg gcgcggtccg tccatcagtt caaacacgcg ctcaccagca acaacagcct
     3541 gttgcagcat cgcctgttgc gtggtcagtt cgattaaggg ttcgttaagt cgcccaagat
     3601 agctgataaa cgcatacaac acgcccactt caatggtgcc gctggcggag aagccaaaca
     3661 gcatcaataa gccacaaaga atgagcgatg aaaacagact cagcagcgga cgcagcagaa
     3721 aaccgtcgag gcgcagggtt tgcatcctag ccatatagtg tgaacgactg gcctccccca
     3781 tacgttcgcc aaatcgcgcc tgctgacgaa actgctggat aacgctcatg ccattgatga
     3841 tttcgttaaa gccgtcgttg atatccgcca gataggcgcg cacacggcgg acaatcggcg
     3901 tgctgtaacg ctggtatatc accattacca ccagcaccac cgggaaaatc attatcgcca
     3961 ccagcgccat tcgccagtcg aggctgaaca tcgccaccag catcgcgccc accagtgcgg
     4021 cactgcgcag gacagttgcc actacggtaa cgtagagatc gcggatcact tcagtgtcat
     4081 tagtgacgcg ggaaatcacc tgcccgacgg gttgggtatc aaactcgctt aatggctggc
     4141 gtaacgcagc atccatcacg tcggtacgca actgttgcac tgcgcctact gccgcccgat
     4201 taaacagcag cgactgcgcg taatgtagcc cggcggcaaa cagttgtagc ccaacatacg
     4261 ccgcagccag ccctgcaacc actttcaacg gcaggttatt tttcgctacc atattgtcga
     4321 taaaatagct gataagcagc ggcccactga cttctgccgc cgccgcaacc cacatcatca
     4381 ggaccgcaat ccccagcggt ttacgccacg gcgaaccata cgctaacagg cgcttgagag
     4441 tcggccacag ttggctaaaa ctacgcatcg acggcctcct cgcgaatttc cggaacgtcg
     4501 tcgagcgccg cctccagttg ttgatagcga tacatatcgc gataccaacc gctttgttgc
     4561 gccagctcat catgattgcc acgctgggcg atatgtccgt gctgcatcac aataatttca
     4621 ctggcttccg tcagtgccga aaggcgatgg gcactgatga ttaccgttct tccctgtccc
     4681 cactgacgca ggttatgcag gatctggtgc tctgtgcgtc cgtccaccgc cgaaagcgca
     4741 tcatcaagga tgaggatttc cgcgttgact aataacgctc gagcaatgga gatacgctgt
     4801 ttttgcccac cggaaagcat cacgccgcgc tcgcccactt ctgtatcgta accttgcggt
     4861 agacgcaaaa tatcgtcatg tacgctggct aaccgcgcga catgctcaat ctcttgctgg
     4921 gtggcattcg ggcaacccag cgcgatgtta ttcgccacgg tgtcagaaaa aaggaatggc
     4981 gtctggctga ccaccgccag gcggctacgc cagctatcga gttgtaactt cgtcagagga
     5041 atatcatgaa agcgaatatc cccctcgctg acgtcgaaat gacgctgaat gagcgacaac
     5101 agggtacttt tgccggaacc agtcggcccg caaataccca gcatctgacc gggtttcagg
     5161 gcgaaattga cgttttccag cgcaggatgg tcagtctgcg gatacgtgaa ctggtgaata
     5221 tttacatcca gttcgccacg cccttccggc acgggttcgc taccatcgtt caccaccggc
     5281 gcttccgcca gcatggcgcg aatacggctg tatgcagcac taccacgttc cacaatgtta
     5341 aacatccatg ccagcgccag cattggccaa atcatcagac ctaaatacat cataaaactg
     5401 gtgagctggc ccagcgttaa actgccctgc accaccatcc aactaccgcc gccaatcgcc
     5461 agcaagttcg ccataccaat cgcgatatag atggtcgggt cgaagcgagc atcaatacgc
     5521 gccacccgca tgtttttttt gccggtatct tcggcatccg cggcaaataa cgccgactgg
     5581 cgatcttcca gaccaaaggc tttgatcatg cggatactgg tgaggctttc ctgggtgcgg
     5641 tcattaagac tggaaaacgc cgcctgtgcc agcttaaagc gttcatgcaa ggcatcgccg
     5701 ttgcgcttaa tcatgatcgc catcactggc atcggtaaca gggcaaataa ggtcaactgc
     5761 cagctaattt gcgtagacat cataatcaac acagcgcagc ccattaccag tgaatccacc
     5821 agcgtcagca ccccttctcc ggcggcaaac acgacgcgat cgacgtcatt ggtcgcacga
     5881 gccatgagat cacccgtgcg atgacgcagg taaaactccg ggtgctgccg gcttagctga
     5941 cggtaataat cttcacgcag ttcaacagcc agttggtaag acgcgccaaa cagcaatacg
     6001 cgccagacat aacgcaggag ataaaccaca acggcaatca gcaccatggt ggcgatccac
     6061 atcaggctca gcccggtagt aaagtgttgt tctgtcacgc catcgacaac aataccaacc
     6121 acttttggcg gaaccagttg cagcatcgcg ataatgacaa gcaaggcgac agccccaaga
     6181 tagcgacgcc attcccgacg gaaataccag cttaattgag caaataatcg cacgcgttgt
     6241 gtcctgacct gattctggat atttattcga tgggtaaaga agtggtgtat ttaatctgtt
     6301 ccatcgcgaa gctggaagtg acgtccgaca gccccggcac gctgtttacc agacgcttat
     6361 aaaactcgtc gtagcgtttc atgtcggcaa cctggacgcg catcagataa tcgtattcac
     6421 cagccatgcg ccagaccccc agcacttctg gcatttcggt aaccaccgtg acaaagcggc
     6481 aataccattc gctgctgtga tgttgcgttt ttatcagcac aaaggcggtc aggccgaggc
     6541 ctattttttc cggatccagc agggcgactt tgccgataag gataccgtcg tcctccagcc
     6601 gtttcaggcg cttccagcag ggggtggttg tcagattaac ggcttcagcc agtgcctgca
     6661 aagagagggt gcaatcctgc tgcagtaagg ccagcagctt acggtcaatt ttatctaaca
     6721 tagcccttcc acggagaatt tttttctctt ttaattctat tttaaaggtg aaatcgcgac
     6781 aatttattct gtgcggttta aacaccacgg cacaaaatga caaattcaac gcaaaaaaaa
     6841 accgggcaat tgcccggtgc tggaagggat ctcgttattc gggggaataa ggtagatgtg
     6901 gatagtccag ccagtgcgtc aaatagtgag agacagcctg atttcggcaa tgtccaatca
     6961 ccggtaaatg cgggagctcc gcgcgcagtt gcggcatcgc attgcccata ataaacccgc
     7021 taccgacgct gcctaacatt tcgcgatcgt tcatcgcatc accaaaggcc atgcaatcgc
     7081 gcaacgataa acctaaatgt tgggtccgca ccgtcaatgc agcgccttta ttgcagccca
     7141 ccggcagcac ttcgaggcaa tccgtggcgg aaaaacacaa atgtgcacgc tcgcctaatg
     7201 cttcgtatag ctggatctgc aagcgtgtaa gatcgtcgtg atcgccacag aagcagatct
     7261 tggtgacgct gccgagtggc atttttttga catcgattat ctgataacga aaaccgctat
     7321 agacaaatac ctgcaacaac gcagggctct ctttcccggt aaaccaaccg tcgtcattga
     7381 agatatgcat gctggctcgg gtatcccatt gctgatacag caccagctcc gcgacatccg
     7441 caggtaaatc atcacgatgt aaaagttcac cttccagaga atgcacgcgc gttccgttgc
     7501 cggtaatcaa atatgcatcc agcgatagcg ccccgagaat atgctgcatc tccagcgcat
     7561 gacgccccgt ggcaaaagtg agggtaatgt cgcgttcacg cagtcgcgcc aaagtagaga
     7621 gggttttctc acctaaatga tggtcaggca tcaataaagt gccatccata tcaaatgcta
     7681 ccagacgagc catttctttc tccactctgt gacacggtta atggtgattg agtatcacct
     7741 gatatatacg gaagtaatag tgaatagtta aataatattg ttccgggttt atatgcgatt
     7801 gctcaaccgt cttaaccagt atcaacgtct gtggcaacct tcagccgcaa accgtcaccg
     7861 tcagcgaact ggccgaacgc tgtttttgca gcgaacgcca tgttcgtacg ctgttgcgtc
     7921 aggcacagga ggcgggatgg ctggagtggc aggcgcagtc aggacgcgga aagcgcggac
     7981 aattacgctt tctggtcacg ccggaatcgc tacgcaatgc gatgatggaa caggcactgg
     8041 aaaccggaaa gcagcaagat gtgctggagc tggcgcaact ggccccaggt gagctgcgca
     8101 ctctgttaca gccatttatg ggcggacaat ggcaaaacga tacacccacg ttgcgtattc
     8161 cctactatcg cccgctcgaa ccgctacaac caggcttttt gcccggccgt gccgagcagc
     8221 atctcgccgg gcagatattt tccggcctga cccgcttcga taataatact cagcgcccga
     8281 ttggcgattt agcgcatcac tgggaaacct ctactgacag gttacgctgg gacttttatc
     8341 ttcgttcaac cctacactgg cataacggcg atgcagtaaa agcctcacac ttacaccagc
     8401 gattattgat gctgttacaa ctgccagcac tggatcaatt atttattagc gtgaagcgta
     8461 ttgaagtcac ccatccgcag tgtctgacct tctttttaca tcgccccgat tactggcttg
     8521 cgcaccggct ggcgagctat tgcagccatc tggcgcatcc gcaattccca ctgatcggca
     8581 ctggtccttt tcgcttaaca caattcacag cggagctggt gcgcctggag agccatgatt
     8641 attaccattt gcgtcatccg ctgcttaaag cggttgagta ctggataact ccgccgcttt
     8701 tcgaaaaaga tttgggaacc agttgtcggc atcccgtgca aatcaccatc ggcaaaccgg
     8761 aggagctgca acgggtcagc caagtcagta gtggcatcag tttaggtttt tgctatttaa
     8821 cgttgcgcaa aagtccccga ctctccctct ggcaggcgcg aaaagtgatc tccattattc
     8881 atcaatccgg tttattacaa acgttagaag tcggagaaaa cctgatcacc gccagtcatg
     8941 cattactgcc aggctggact attccgcact ggcaagtacc ggatgaagtc aaactaccga
     9001 aaaccttgac gctggtttat cacctaccga tagaacttca taccatggca gaacgcctac
     9061 aggcgacact ggcagcggaa ggctgtgaac tcacaattat ttttcataac gcaaaaaact
     9121 gggacgacac gaccctactg gcacacgcag acctcatgat gggcgacaga ttaattggcg
     9181 aagcaccgga atatactctg gagcaatggc tacgttgcga tccactatgg ccacatgttt
     9241 tcgacgctcc agcatatgca catctgcaat cgacgctgga cgcggtgcaa gtaatgcctg
     9301 atgaggaaaa ccgatttaat gccctgaaag cggtttttag ccagttaatg gcagacgcga
     9361 cgctgacgcc gctgttcaac tatcactatc gcattagtgc ccctcccggc gtgaacggtg
     9421 tgcgactgac accgcgcggc tggtttgaat ttaccgaagc ctggcttccc gcgccgtcgc
     9481 aatgaatagc tggtccgggt taatcgcagg cgttaccata acgcttttat tgttcaagca
     9541 ggattatcta tgaaacgtgc tgtcgttgtg ttcagtggag gccaggattc caccacctgt
     9601 ctggtgcagg cattacaaca atatgatgaa gtccattgcg tgacgttcga ttacggtcag
     9661 cgacatcgcg cagaaatcga cgtggcacgc gaactggcgc tgaaactggg agcacgcgcg
     9721 cataaggttc ttgatgtaac gctgctcaac gagctggcgg tcagcagcct gacacgtgac
     9781 agcattccgg tgcctgatta tgaacctgaa gccgatggca tcccgaatac gtttgtccca
     9841 gggcgtaata ttttgttcct gacgctggcg gcaatatatg cgtatcaggt aaaagcagaa
     9901 gccgtaatta ccggcgtctg tgaaacggat ttctccggct acccggattg ccgcgatgag
     9961 tttgtgaaag cactaaacca tgccgtcagt ttgggcatgg cgaaagatat tcgttttgaa
    10021 acgccgctga tgtggattga taaagcggaa acctgggcgc tggcagatta ttacggcaaa
    10081 ctggatttag tccgtaacga aacgttgacc tgctataacg gcattaaagg cgacggttgc
    10141 ggtcattgtg cggcatgtaa tttacgcgcc aacggtttga atcattatct ggccgataaa
    10201 ccgacggtga tggcagcgat gaagcagaaa accgggttga ggtaattatt ccgggtgtcg
    10261 ccggatgcgg cttgagcatc cggcaccaca aaacgtttac ttaaccatct gctccagctt
    10321 ttcgcgcaat tccccttcca gagctaatgc tttctgcgtt ttaagatcaa tacaaacaaa
    10381 cgtaataagc gcatccgcta ccacctgccc ttccggctcc agtgtaatga cctggtttaa
    10441 gatgccgctt ttaccgttta attgctgcaa ctgactggta atagttaaca ggtcgcttaa
    10501 taccgctgga cgacgatagt taatattgat attgaccacg acgaaggcga tgttatgggc
    10561 cgtcatccac tgaaaactgt cgctattttc caccccatcc cagcgggctt cttcgagaaa
    10621 ttcaaggtag cgggcgttgt tgacgtgctg gtaaacgtcg agatgatatc cacgaacttt
    10681 gatttgtgtt tgcatagcgc aataacctta cgttgttgtt ataagtacag aggtcataac
    10741 tggtatgacc tcttcagtct ggcaaaaaat tgccactatg caaattaatt acagggttaa
    10801 taccgccaga ttacgttcca ccagcgaatt gcccatcccc ggcacctgct ttagatcctc
    10861 caccgtttta aacggaccgt actcttcgcg ataactgaca atcgcctgcg ctttcttcag
    10921 gccaacgcca ttcatcgcgc gggctagctc ttccgcgctg gcattattaa tgctgacccg
    10981 ggtgccttct tcgtcactgg ctttcgccgg tactgctgct ttactttgtg ctgcaggagc
    11041 ttccgctttg gtttctaccg ccgtcggttt cgccacagaa gctgccgcca gcgcgctatg
    11101 agacattccg gcacaggcca gggacagtgt aatgagcagt gctttaattc cgtgtttcat
    11161 actgttttct ccttgtttgt taacagtgga atcaccttaa cgggagtgaa aacagtaaac
    11221 aaatggcaat gttcagaaat ggaaaaggcc gcgaaagcga ccttttcttg ttacagctgc
    11281 aacgcatttt tttgcgaggc ggcttcagga ttattgctgt tccagcgcat cgccaatttt
    11341 gattttcgcc tctttacgca ggttactcat cagagcttca aagacgattt gtgcgttgtt
    11401 ctgggtgata ccctgcacca tcgctttttt ctgatcttcc ggcattgaac cttgtttcac
    11461 ttcatccagc gccagcagaa ccacattacc ttgcatatcg gtcgccatac cgtagctcgg
    11521 tttgtctttc gctggcagtg gcagtgcaaa cgccgcctgg ctaatcgggt cacgaccgga
    11581 acggcttaag gttttcggct cgccaaattt cagaccggca gcctgcatag cttccgcacc
    11641 tttgccggct ttcaaatcaa ccagcagttt ctcagcatcc actttcgcct gttgttcagc
    11701 tttgttgtgc tgaaccagtg ccttaacttg ttcctgaaca tctgccaacg gtttcaccgc
    11761 ttccggtttg tgctcgctga cgcgcagcac gaatgcgcgg tcgccgtcta cggtgatgat
    11821 gtcagagttg atgcccgacg cgccgttttc acctaccaga ccgccgttaa agatagcgtc
    11881 ggcaaccggc ttgaagttca actcttccgg caggttatct ttgctgaacc aacccgtctg
    11941 agtggcttta acgccggcag cttgctctgc accggccaga gactcggtgt cgttgcttgc
    12001 cgcatcgctc actttctgct gcagcgcata gtacgcatcg agggcttttt cgtgtttcac
    12061 tttcgccgca atgtcgtcac gtacttcgtc taacgatttc actttcgctg gctgaatgtc
    12121 gtccagacgt acaatcagga aaccgaccga agatttgatg acaccagaca gttggccttt
    12181 ttctttcaga ccagcatttt tcagttcgtc ggggatagtg gcatcttcta accaacccat
    12241 atcgccgccg ttacgggcag agataatatc ggcagatttt tctttggcta atgcagcaaa
    12301 atcaccgcct ttattcagct catcaagtac cgctttcgct tcatcttcag ttttggtctg
    12361 gatgatgctg tagcgggtac gctgcggctg ggtgaattga tcctgatgct gatcgtagta
    12421 gctctggata tccgcatcgc taaccggttg ctgcatcgtt gcggcaacca gcttgatgta
    12481 actcacgcgg aattgttccg gcgtcatgaa attgtttttg ttttgttcgt agtagctggc
    12541 aatctcctgt tcggtcacag gctgcttcgc cgccagcgcg ttaacatcga tagtcgcctc
    12601 acgcaccacg cgttgttgcg cgaccagtgc cgccagctcg tcggtttcac ctttcagcat
    12661 aaaatcggta ccggcaacgc cgttaatcag ctgttgggta gtgagctggt tacgcagcgc
    12721 ctgggcgtac tgatcggcgg tcatccccat ctggttgagg ataccgttat agcggctgtt
    12781 atcaaatttg ccgtcaacct ggaaggctgg ggtcgcgaaa atcgcctgtt taacctgctc
    12841 atcactgata cccagtttca gctcacgagc gtactgatcc aacagcgcct cgtcgatcag
    12901 acgattcagc acctgttgac gcagggtttt catatagcct tcgttcgctg ccagctcgga
    12961 gtattgatcg cccagctgtt gctgcatacg attacgctcg ctgttaaagg cgttctcgaa
    13021 ctgcccacgg ctgatttcct ggtcattcac ttttgcggcg tagttattgc ctccgccaat
    13081 caggtaacca ctcacgccgg tcaatatgaa cgacacgata atgataccga aaataatctt
    13141 gagcacgaga ctgtttgcag ccgtgcgtaa gctgtccatc atggtgtaac aacactccgc
    13201 tgtagatgac tgtgtacctc acgcaacgta tcgatgcgcg caaaggccta ttgtgacaag
    13261 aaacgggggc aattgtcagc ccacaagaca cagaaagtca ccgaatacaa ataaaaaagg
    13321 cacatcagta gatgcgccct tgaacttcgt cacatcccca ctggggacaa cgcttagttt
    13381 accgcgtctt tcagtgcttt acctgcacgg aagctcggta ctttagcggc agcgatggtg
    13441 atctctttac cggtctgcgg gttgcggcca gtacgggcag cacgctcttt aacggcaaaa
    13501 gtaccaaaac ctaccagtgc tacatcatcc ccttctttca gagattcagt tacggaagca
    13561 ataatagcat ctaacgcacg gccagccgca gctttagaga tatcagcccc tgcagcaatc
    13621 ttgtcgatca attgagattt attcactctt ctcttcctct ttataattta tatcgcactt
    13681 gaatccttca aggtacgaac gcgcagcagt tatatcaggc cagccatccc cttacaacac
    13741 cccttaataa gggcaagccc gatccgctat ctaacttagc gagacaaaaa aaggctggca
    13801 agcccgaatt agcctgccag ccctgttttt attagtgcat tttgcgcgag gtcactattt
    13861 tgcagtcaca acctgcatgc cagacggttc attttgcagc gccagagtca gaacttcctc
    13921 aatgcgcttc acaggatgaa tgtccagatc ggcaattacg ttgtcaggaa tctcttccag
    13981 atcgcgttta ttttcgaacg gaattagcac tgttttaatc ccgccgcgat gcgctgccag
    14041 gagtttttct ttcaaaccac cgatcggcag tacctgacca cgcagagtga tctcaccggt
    14101 cattgccaca tcggcacgaa ccgggttacc ggtcaggcaa gaaaccagcg cggtgcacat
    14161 agcaataccg gcactcggac catctttcgg cgtcgcacct tccggtacgt ggacgtggat
    14221 gtcacgtttt tcgtaaaaat cagggttgat ccccagtttt tccgcacgcg cacgaaccac
    14281 cgttaacgcc gcctgaatag actcctgcat cacttcgccg agcgaaccgg tataggtcag
    14341 tttgccttta cccggaacac atgcggtttc aatggtcagc aagtcaccgc ccacttccgt
    14401 ccacgccaga ccgattacct gaccgacacg gttttcgtta tcagcgcgac catagtcgaa
    14461 acgctgaaca ccgaggtagt catgcaggtt atcgccgtta atttcgatat gttttaatga
    14521 cttatcgagc agtaactgct taaccgcttt gcggcacagt ttggagattt cacgctccag
    14581 accacgcacg cccgcctcac gggtgtagta acgaataatg ccgataatgg cgctatcgtc
    14641 gacggtcagc tcaccttttt tcagtgcatt acgttcaatc tgcttcggca gcaggtgacg
    14701 tttggcgatg ttcagttttt catcttcggt ataaccggag aggcgaatca cttccatacg
    14761 atccagcagc ggtgccggaa tgttcatgga gttcgacgtc gcgacaaaca tcacgtcgct
    14821 gagatcgtaa tccacttcca ggtagtggtc gctgaacgct acgttctgct ctggatccag
    14881 cacttcaagc agtgcagagg ccggatcgcc tcgcatgtca gaagacattt tgtcgatctc
    14941 atcgagcagg aacagcggat ttttcacgcc cactttcgcc attttctgga tcagtttacc
    15001 cggcatagaa ccgatgtaag tacggcggtg accacggatt tccgcttcat cacgcacgcc
    15061 gcccagcgcc atacggacat atttacgccc ggtggctttg gcaatggact gaccaagaga
    15121 ggttttacct acccccggcg gccctaccag gcagaggatc ggtcccttga ttttgttgac
    15181 acggctttga accgcaaggt actcaaggat gcgatctttc acgcgctcca gaccataatg
    15241 gtcggtatca aggatttcct gcgcctgacg caggtctttt ttgaccttgc tgcgcgcatt
    15301 ccacggtacc tgtaccatcc agtcgatata accacgcact acggtcgctt ctgccgacat
    15361 cggagacatc attttcagct tctgcaactc tgcttccgct ttctcttttg cctctttcgg
    15421 catcttcgcc gcgtcgattt tgcgcttcag ggcttcgttt tcgtccggca cgtcgtccat
    15481 ttcaccgagt tctttctgaa tagctttcat ttgctcgttc agatagtact cacgctggga
    15541 tttctccatc tgctttttaa cgcggttgcg aatgcgtttc tcaacctgca gcagatcgat
    15601 ttccgattcc atcattgcca tcagatattc cagacgttcg ttaacgtcgg acatctccag
    15661 aacggactgt ttgtcagcca gtttcagcgg catatgtgca gcaatggtat ccgccagacg
    15721 cgccggatcg tcgatgctat tcagcgacgt cagcacttct ggtgggattt ttttgttcag
    15781 cttgatgtag ccttcgaact ggctgattgc agtacgcacc agcacttcct gttcccgctc
    15841 atcaatggtc ggcgactcca gatactccgc cttcgcagaa aagtgttcgc cattgtcaga
    15901 gagcgcagaa atacgcgcgc gctgtaaccc ctcgaccagc actttgacgg tgccgtcagg
    15961 cagtttcagc atctgcaata tagaggccac ggtcccgacg gtgaaaagat cgtttacacc
    16021 cggctcatcc gttgaagctt ctttctgcgc gaccagcata atttttttat catggtccat
    16081 cgccgcttcc agacaacgga tagatttttc ccgcccgaca aataagggga tgaccatgtg
    16141 cggataaacc accacatcgc gcagcggcaa tacggggatt tcaatgcgtt cagaacgctc
    16201 aggattcata gagctctctc ttagtttaat ttccgccagg taatcagatg acacgactgt
    16261 gcttcacgcc atctattaac atgtacgtca gtatatgggg atgtttcccc cacattcaac
    16321 gccgagaata gaggaaaaat taaaggggag ataaaatccc ccctttttgg ttaactaatt
    16381 gtatgggaat ggttaattat tcaccagatg cctgttgcgc ttccggctta ccataaatca
    16441 gcaacggttt gctttgacca tcaattaccg actcgtcgat aaccactttt tcgacgtctt
    16501 ccatggacgg cagatcgtac atggtatcga gcagtgcggc ttctacgatg gaacgcaggc
    16561 cacgggcacc ggttttacgc gccatcgctt tcttagcgat agcatccagc gcctcgtcac
    16621 ggaattccag atccacgcct tccagattaa acagcgcctg atactgcttg gtcagggcgt
    16681 ttttcggctc tttgaggatc tgaatcagag cttcttcgct cagttcattc aacgttgcta
    16741 caaccggcag acgaccaatg aactcaggga taagaccaaa cttgatcaga tcttccggtt
    16801 caacctgcgc cagcagctcg ccttcgcttg ctttgtcgga cttcgctttt accgtcgcgc
    16861 caaaaccaat gccggagccg gtttctacac ggtgggaaat cactttatcc agaccggcaa
    16921 acgcaccgcc acaaataaac aggatcttag aggtatcaac ctgcaagaat tcctgctgcg
    16981 gatgtttacg cccaccttgc ggtggaacag cagctaccgt accttcgatc agtttcaaca
    17041 gtgcctgctg tactccttca ccggaaacgt ctcgggtaat ggacgggttg tctgacttac
    17101 gagaaatctt gtcgatttca tcgatgtaga caatcccgcg ctgcgctttc tgtacgtcgt
    17161 aatcgcactt ttgcaacagc ttctgaatga tgttttcaac gtcttcgccc acataaccgg
    17221 cttcggtcag cgtggttgcg tcggccatgg tgaacgggac gtccagcagg cgcgccagcg
    17281 tttcagccag cagcgtttta ccggaaccgg tcggaccgat cagcagaatg ttacttttgc
    17341 ccaactcgac accattgctg gtatcgccgt tgcgcagacg tttgtaatgg ttgtataccg
    17401 cgaccgccag cacttttttc gcctgttcct ggccgataac gtaatcgtcc aggtggttgc
    17461 gaatttcatg cggcgtcggt agcgcactgc gttcacgatg cggtgcaact tctttaatct
    17521 cttcgcgaat gatgtcgtta cataaatcaa cacattcgtc gcagatatac acggatggac
    17581 cggcaatcag cttgcgcact tcatgctggc ttttgccgca aaaagagcaa tacagcaatt
    17641 tgcctgagcc atctttgcgt ttatctgtca tgggtcaaaa cctcttcttt gttctttgtg
    17701 ccgcacacga cgacgcaaat gccattctca ggcgcaagcc gcttacagcg ttgtgccgcc
    17761 ctagataagt atagcggcac agttgcgcct ctggcatcaa ttacgatggg tcagaatcga
    17821 atcgaccaga ccgtattcca ccgcttcagg ggcggaaagg aagcgatcgc gctcggtatc
    17881 acgttcaatc tgttctaatg attgacccgt atgaagcgcc ataagttcat tcatgcgccc
    17941 tttaactttc agaatttcac gggcatgaat ttcgatatcg gtcgcctggc cctggtagcc
    18001 gcccaacggt tggtgaatca tcacgcgcga attcggcagg caaaaacgtt taccttttgc
    18061 ccctgcggtc aacaagaaag cgcccatcga ggccgcctgg cccatacaga tggtgctgac
    18121 atcaggcttg ataaactgca tggtgtcata gatagacatc ccggcagtga tcaccccgcc
    18181 tggggagtta atgtacagat agatatcttt ttctgggttt tccgcttcca ggaacagcat
    18241 ctgcgccaca atcaggttag ccatgtggtc ttcaacctgg ccagtcagaa aaatgacgcg
    18301 ttccttaagt agacgagaat agatatcaaa agagcgctca ccgcgtgagg tctgttcaat
    18361 gaccatcggc accagcgcca tatggggtgc aaagttatct cgttcgccgc tgtatgacat
    18421 ttccgtctcc tggataaaat tgaaaaaaac ctgctgtacc gattgtaacc ttatggacgg
    18481 attatcagct agtccctgtg ttatgggtac ggcatcacca tatttcaagc ataacaatct
    18541 tttgttgtta cgctaacacc gaaagaggct tttcgcacgg ctccatgcaa aattcatgac
    18601 aaaaaaaagc ccaccacctg gcggtgacgg gcctttgtgc gaatttagcg cgttatgctg
    18661 cgtaaattac gcctgctggt tcatcagctc gttgaaagtg gtttcttttt cagtcacttt
    18721 cgctttcgcc agtacagctt caacagcctg ttcttccaga gcaacattgc gcatgttgtc
    18781 catcagttct ttgtttttgc tgtagaactc gataacttct ttcggatctt cgtacgcaga
    18841 agccatctct tcgatcaggc ctttcacgcg ctcttcgtca gctttcagct cgttggtgcg
    18901 gataacttcg cccagcagca ggccaacaac tacgcggcgt ttagcctgtt cttcgaacag
    18961 ttcgcgcggc agttccagag cttgtttttc gttgccaccg aaacgctgtg cagcctggcg
    19021 acgcagaacg tcgatttcgc tgtcgatcag cgcagccggt acgtcgatgt cgttagcttt
    19081 taccagacct tcgatcgcct gagacttaac gcggttacgg atggcgctct tcagctcgcg
    19141 ctccatgttt ttacgcactt cagcgcgcag accttctacg gaaccatctt caacgccgaa
    19201 acgtttgatg aattctgcag tcagttccgg cagttcacgc tcttcaactt tcttcaggtt
    19261 gatagcgaat ttcgctgctt tacctttcag gttttctgcg tggtattctt ccgggaaggt
    19321 cacgtcgatg gtgaactctt cgccagcttt gtggcctttg ataccgtctt caaagcccgg
    19381 gatcatacga ccctggccca tcgccagtac gaaatcagac gctttaccgc cttcgaactc
    19441 ttcgccgtct acagaaccgg tgaagtcgat ggttacgcgg tcttctgctt caacagcgcc
    19501 gtctttttct ttccaggtcg cctgctgttt acgcagagta tccagcatgc cgtcaacgtc
    19561 agcgtcggtc acttcaacga tcggtttttc aacttcgatc gcttccagac cctgcagttc
    19621 aacttccgga taaacttcaa actctacaga gtaagtgaag tcttcaccca gcttgtattc
    19681 gcccggaaca taagtcggtg cgccagccgg attgattttt tctttaatga tggcgtcaat
    19741 gaagttacgg ctcatcaggt cacccagaac gtcctggcgt acagacgcgc cataacgctg
    19801 agcaacgata ttcattggca ctttgccttt gcggaagccg tcaatacgta cttttttcgc
    19861 aacgttgacc agctcgcttt taacagcggt ctcgatgctg tcagcagcga tagtaatcgt
    19921 tacacggcgg ccaaggcctt gagtggtttc aactgaaact tgcatcttgt tacctcaaaa
    19981 aatcacagtg ctcggtcaac tcttaccccg caagcacagg ttgttggctt cgcggaaccg
    20041 ggatgttctt ataatcaatc acattccctg ttaccagaat catcccgaaa gcattcaaat
    20101 aggacgcggc attatagcgg catcactttt atgagtcgag aacggttatt gcgcgctgtc
    20161 gcgtattttt tcacaattcc cgggcaattt tcgtctgaat gctgggcaaa aagcgcaaaa
    20221 tccagacaaa acaaaacggc ccgcaggccg tttttcataa aatctttagc aacatactgg
    20281 aaaagcgccg acagttgcaa atgcgttttt acgcgatgct tcctgctcca cgacagggag
    20341 gagaggcaaa gacggtgtcc tgcaaccctt cccactcctt aatagtgtaa gtatgcagag
    20401 ccagcgcatg aacggtagta gagagttcct ccgctaaagt actgtaaatc attcgatgac
    20461 gattcagaaa acgttcaccc gtaaaacgat cgctgaccag cacaacttta aaatggcttt
    20521 cagagccggc tgggacattg tgacgatagc tttcatccac tacttcgagg aatacgggtt
    20581 ggaacgccgc ccttaatttt tcttctatcc gctcacgtat catcatgaaa tatctcctcc
    20641 gacaacgctg agatgtcatc catcccttta aatactagcc gcttttaccg tttccattgc
    20701 agcttaacaa caaatattta ggtttcgtcc tggtttttcc gtcaagcgta tgaagtttac
    20761 tgatttaaag attgaaacac aggcctcgca tcagcgtttc ttttaccagg ggtgattatt
    20821 tcgtcaacat gatgaattta catgccttac ccacttcccc tcgccgttgg caatgttatg
    20881 atggcgggaa tttttaccaa acacgcttaa gagtctctga gagcccgaac atgttcaaaa
    20941 aaatcctctt cccgttagtt gctctgttta tgcttgcagg atgcgcaaaa ccgccaacaa
    21001 ctattgaagt ttccccgacg attacactgc cacagcagga tccaagcctg atgggcgtca
    21061 ccgtaagcat taatggtgcc gatcagcgta ccgatcaggc gctggcaaaa gtcacccgcg
    21121 ataatcaaat cgttaccctg accgcctccc gcgctctgcg tttcctgctg caagaagtgc
    21181 tggaaaaaca gatgaccgcg cgtggttaca tggttggtcc gaatggcccg gttaatctgc
    21241 aaatcatcgt tagccaactg tatgctgacg tgtcccaggg caacgtgcgc tacaacatcg
    21301 ctaccaaagc ggatatcgcc atcatcgcta ccgcgcagaa tggcaacaaa atgaccaaaa
    21361 actatcgtgc cagctacaac gttgaaggtg cgttccaggc ctccaacaaa aatatcgccg
    21421 atgcggttaa cagtgtgctg accgatacca tcgctgatat gtctcaggac accagcattc
    21481 acgaattcat caagcagaac gcgcgttaat ttctgccctc tggcccggtg caagccgggc
    21541 ctgtagacgc ccatgtccag tcaatattta cgtatttttc aacagccgcg ttcagccata
    21601 ttgctgatcc tgggttttgc ttccgggcta ccgctcgccc tgacatccgg caccttacag
    21661 gcctggatga cggtcgaaaa tatcgatctc aaaaccattg gtttcttctc tctggtaggc
    21721 caggcttacg tttttaaatt cctctggtca ccgctgatgg accgctacac gcccccattt
    21781 tttgggcggc ggcgcggttg gctgctcgcc acgcaaatcc tgttattagt cgccattgcg
    21841 gcgatgggtt ttctcgaacc aggcacccaa ctccgctgga tggcggcgct ggcggtggtg
    21901 atcgcttttt gctctgcctc ccaggatatt gtcttcgatg cgtggaaaac cgatgtgctc
    21961 ccggcagaag aacgtggtgc gggcgcggca atcagcgtgc tgggttaccg tttagggatg
    22021 ctggtttccg gcggcctagc cctgtggctg gcagataaat ggttgggctg gcagggcatg
    22081 tactggttga tggcggcgct gttgatcccc tgtattatcg cgacgttgct tgcaccagaa
    22141 ccaaccgaca ccattcctgt gcccaaaacg ctggaacaag cggttgttgc acctctgcga
    22201 gatttctttg gtcgcaataa tgcctggctt attttgcttc ttatcgtgct gtataagctg
    22261 ggcgacgcat tcgccatgag cctgacaacc acgtttttga ttcgcggcgt cgggtttgat
    22321 gcgggtgaag taggcgtggt aaacaaaacg cttggcttac tggcgaccat tgttggcgca
    22381 ttgtacggtg ggattttgat gcagcgcctg tcgctgttcc gggcgctgct gattttcggc
    22441 attttacaag gtgcgtctaa cgctggttac tggctgctgt cgattactga taagcatctc
    22501 tacagcatgg gcgcagccgt ctttttcgaa aacctctgtg gcgggatggg cacatcagcc
    22561 tttgtcgcgc tgttaatgac gctgtgtaat aagtcatttt ccgctactca atttgccctg
    22621 ctctcagcgc tttccgctgt agggcgagtt tatgtcggcc ccgtggcggg ttggtttgtt
    22681 gaagcacacg gctggtcgac attctatcta ttctccgtcg ccgctgccgt accggggctt
    22741 attttgctgc tggtttgccg ccagacgctt gaatatacac gagtaaatga caactttatc
    22801 tcccgtaccg aatatccggc aggttatgcc tttgccatgt ggacactggc ggcgggcgtc
    22861 agcctgttgg ccgtgtggtt acttctgttg acgatggacg cgctggattt gacgcacttc
    22921 tctttcctgc ctgctctgct ggaagtcggg gttttagtcg ccctttctgg cgtcgtgctt
    22981 ggtggtttgc tggattatct ggcgctacga aaaacgcatc tgacgtaatc tgtaaatatt
    23041 atttagctgt agtaatcact cgccgtaatt attacggcta aataaacatc agcatcgctt
    23101 attaatattt gttcttttgt gcggcttagc gtttggaaat tattgacgcc atttataaat
    23161 tctatttttc ttacacgatt cagctaatga gtctttattt tctcaccacc cagttgtcac
    23221 tttaatgata attatttgtt aaataattgt tttatttcgc attggttata ccaattgccc
    23281 accaagactc cttctgcact cc
//
LOCUS       DAJTRC010000060        22413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.060, whole genome shotgun sequence.
ACCESSION   DAJTRC010000060 DAJTRC010000000
VERSION     DAJTRC010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.060"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            83..2683
                     /locus_tag="OO357_002532"
     CDS             83..2683
                     /locus_tag="OO357_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis usher protein"
                     /protein_id="HCR5887441.1"
                     /translation="MYRTHRQHSLLSSGGVPSFIGGLVVFVSAAFNAQAETWFDPAFF
                     KDDPSMVADLSRFEKGQKITPGVYRVDIVLNQTIVDTRNVNFVEITPEKGIAACLTTE
                     SLDAMGVNTDAFPAFKQLDKQVCVPLAEIIPDASVTFNVNKLRLEISVPQIAIKSNAR
                     GYVPPERWDEGINALLLGYSFSGVNSIHSSADSDSGDSYFLNLNSGVNLGPWRLRNNS
                     TWSRSSGQTAEWKNLSSYLQRAVIPLKGELTVGDDYTAGDFFDSVSFRGVQLASDDNM
                     LPDSLKGFAPVVRGIAKSNAQITIKQNGYTIYQTYVSPGAFEISDLYSTSSSGDLLVE
                     IKEADGSVNSYSVPFSSVPLLQRQGRIKYAVTLAKYRTNSNEQQESKFAQTTLQWGGP
                     WGTTWYGGGQYAEYYRAAMFGLGFNLGDFGAISFDATQAKSTLADQSEHKGQSYRFLY
                     AKTLNQLGTNFQLMGYRYSTSGFYTLSDTMYKHMDGYEFNDGDDEDTPMWSRYYNLFY
                     TKRGKLQVNISQQLGEYGSFYLSGSQQTYWHTDQQDRLLQFGYNTQIKDLSLGVSWNY
                     SKSRGQPDADQVFALNFSLPLNLLLPRSNDSYTRKKNYAWMTSNTSIDNEGHITQNLG
                     LTETLLDDGNLSYSVQQGYNSEGKTANGSASMDYKGAFADARVGYNYSDNGSQQQLNY
                     ALSGSLVAHSQGITLGQSLGETNVLIAAPGAENTRVANSTGLKTDWRGYTVVPYATSY
                     RENRIALDAASLKRNVDLENAVVNVVPTKGALVLAEFNAHAGARVLMKTSKQGIPLRF
                     GAIATLDGIQTNSGIIDDDGSLYMSGLPAQGAITVRWGEAPDQICHISYQLTEQQINS
                     AITRMDAICR"
     gene            2674..3657
                     /locus_tag="OO357_002533"
     CDS             2674..3657
                     /locus_tag="OO357_002533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5887442.1"
                     /translation="MQIIFGEKCVLLLRLFFAAVLMLWCAQTAAYIGQCHTTQGNPYI
                     GVNFGVKTLDEEENTAGVVKDKFYQWNESNDYYVSCDCDKDNVRSGRWAFAADSPLVY
                     LGDNWYKINDYLAAKVLLQVKGSSPTAVPFENVGTGADTRWHICDPGGQRLGGQGASG
                     NSGSFSLKILQPFVGSVVIPPMALARLFECYNIPAGDSCTTTGTPVLVYYLSGTINSL
                     GSCSVNAGETIEVDLGDVFAANFRVVGHKPLGARTAELAIPVRCNTGNAGLVNVNLSL
                     TATSDPSYPQAIKTSRPGVGVVVTDSQHNIISPAGGTLPLSIPDDADSIRE"
     gene            3755..4295
                     /locus_tag="OO357_002534"
                     /pseudo
     CDS             3755..4295
                     /locus_tag="OO357_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706865.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
     gene            4303..4818
                     /locus_tag="OO357_002535"
     CDS             4303..4818
                     /locus_tag="OO357_002535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415463.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5887443.1"
                     /translation="MLKRIIWILFLLGLTGGCELFAHDGTVNISGSFRRNTCVLAQDS
                     KQINVQLGDVSLTRFFHGNYGPEKSFIINLQDCGTDVSTVDVTFSGTPDGVQSEMLSI
                     ESGTDAASGLAIAILDDAKILIPLNQASKDYSLHSGKVPLTFYAQLRPVNSDVQSGKV
                     NASATFVLHYD"
     gene            4784..5521
                     /locus_tag="OO357_002536"
     CDS             4784..5521
                     /locus_tag="OO357_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial chaperone"
                     /protein_id="HCR5887444.1"
                     /translation="MPARHLYFIMTNTWNRLALLIFAVLSLLVAGELQAGVVVGGTRF
                     IFPADRESISILLTNTSQESWLINSKINRPTRWAGGEASTVPALLLAAPPLILLKPGT
                     TGTLRLLRTESDILPVDRETLFELSIASVPSGKVENQSVKVAMRSVFKLFWRPEGLPG
                     DPLEAYQQLRWTRNSQGVQLTNPTPYYINLIQVSVNGKALSNAGVVPPKSQRQTNWCQ
                     VIAPCHVAWRTINDYGGLSAKKEQNLP"
     gene            5632..6642
                     /gene="pyrD"
                     /locus_tag="OO357_002537"
     CDS             5632..6642
                     /gene="pyrD"
                     /locus_tag="OO357_002537"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HCR5887445.1"
                     /translation="MYYPFVRKALFQLDPERAHEFTFQQLRRITGTPFEALVRQKVPA
                     KPVNCMGLTFKNPLGLAAGLDKDGECIDALGAMGFGSIEIGTVTPRPQPGNDKPRLFR
                     LVDAEGLINRMGFNNLGVDNLVENVKKAHYDGVLGINIGKNKDTPVEQGKDDYLICME
                     KIYAYAGYIAINISSPNTPGLRTLQYGEALDDLLTAIKNKQNDLQAMHHKYVPIAVKI
                     APDLSEDELIQVADSLVRHNIDGVIATNTTLDRSLVQGMKNCDQTGGLSGRPLQLKST
                     EIIRRLSLELNGRLPIIGVGGIDSVIAAREKIAAGASLVQIYSGFIFKGPPLIKEIVT
                     HI"
     gene            6816..7358
                     /gene="zapC"
                     /locus_tag="OO357_002538"
     CDS             6816..7358
                     /gene="zapC"
                     /locus_tag="OO357_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415466.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapC"
                     /protein_id="HCR5887446.1"
                     /translation="MRIKPDDNWRWYYDEEHDRMMLDLANGMLFRSRFARKMLTPDAF
                     SPAGFCVDDAALYFSFEEKCRDFNLSKDQKAELVLNALVAIRYLKPQMPKSWHFVSHG
                     EMWVPMPGDAACVWLSDTHEQVNLLVVESGENAALCLLAQPCVVIAGRAMQLGDAIKI
                     MNDRLKPQVNVDSFSLEQAV"
     gene            complement(7355..8464)
                     /gene="ycbX"
                     /locus_tag="OO357_002539"
     CDS             complement(7355..8464)
                     /gene="ycbX"
                     /locus_tag="OO357_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-N-hydroxylaminopurine resistance protein YcbX"
                     /protein_id="HCR5887447.1"
                     /translation="MATLTRLFIHPVKSMRGIGLTHALADVSGLAFDRIFMITEPDGT
                     FITARQFPQMVRFTPSPVHDGLHLTAPDGSSAYVRFADFATQDAPTEVWGTHFTARIA
                     PDAINKWLSGFFSREVQLRWVGPQMTRRVKRHNTVPLSFADGYPYLLANEASLRDLQQ
                     RCPASVKMEQFRPNLVVSGASAWEEDSWKVIRIGDVVFDVVKPCSRCIFTTVSPEKGQ
                     KHPAGEPLKTLQSFRTAQDNGDVDFGQNLIARNSGVIRVGDEVEILATAPAKIYGAAA
                     ADDTANITQQPDANVDIDWQGQAFRGNNQQVLLEQLENQGIRIPYSCRAGICGSCRVQ
                     LLEGEVTPLKKSAMGDDGTILCCSCVPKTALKLTR"
     gene            8708..10816
                     /gene="rlmKL"
                     /locus_tag="OO357_002540"
     CDS             8708..10816
                     /gene="rlmKL"
                     /locus_tag="OO357_002540"
                     /EC_number="2.1.1.173"
                     /EC_number="2.1.1.264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 23S rRNA
                     (guanine(2069)-N(7))-methyltransferase RlmK/23S rRNA
                     (guanine(2445)-N(2))-methyltransferase RlmL"
                     /protein_id="HCR5887448.1"
                     /translation="MNSLFASTARGLEELLKTELENLGAVECQVVQGGVHFKGDTRLV
                     YQSLMWSRLASRIMLPLGECKVYSDLDLYLGVQAINWTEMFNPGATFAVHFSGLNDTI
                     RNSQYGAMKVKDAIVDAFTRKNLPRPNVDRDAPDIRVNVWLHKETASIALDLSGDGLH
                     LRGYRDRAGIAPIKETLAAAIVMRSGWQSGTPLLDPMCGSGTLLIEAAMLATDRAPGL
                     HRGRWGFSGWAQHDEAIWQEVKAEAQTRARKGLAEYSSHFYGSDSDARVIQRARTNAR
                     LAGIGELITFEVKDVAQLTNPLPKGPYGTVLSNPPYGERLDSEPALIALHSLLGRIMK
                     NQFGGWNLSLFSASPDLLSCLQLRADKQYKAKNGPLDCVQKNYHVAESTPDSKPAMVA
                     EDYANRLRKNLKKFEKWARQEGIECYRLYDADLPEYNVAVDRYADWVVVQEYAPPKTI
                     DAHKARQRLFDIIAATISVLGIAPNKLVLKTRERHKGKNQYQKLGEKGEFLEVTEYNA
                     HLWVNLTDYLDTGLFLDHRIARRMLGQMSKGKDFLNLFSYTGSATVHAGLGGARSTTT
                     VDMSRTYLEWAERNLRLNGLTRRAHRLIQADCLAWLREANEQFDLIFIDPPTFSNSKR
                     MEDAFDVQRDHLALMKDLKRLLRAGGTIMFSNNKRGFRMDLDGLAKLGLKAQEITQKT
                     LSQDFARNRQIHNCWLITAA"
     gene            10828..12735
                     /locus_tag="OO357_002541"
     CDS             10828..12735
                     /locus_tag="OO357_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5887449.1"
                     /translation="MSLISMHGAWLSFSDAPLLDNAELHIEDNERVCLVGRNGAGKST
                     LMKILNREQGLDDGRIIYEQDLIVARLQQDPPRNVEGSVYDFVAEGIEEQAEYLKRYH
                     DISRLVMNDPSEKNLNELAKVQEQLDHHNLWQLENRINEVLAQLGLDPNVALSSLSGG
                     WLRKAALGRALVSNPRVLLLDEPTNHLDIETIDWLEGFLKTFNGTIIFISHDRSFIRN
                     MATRIVDLDRGKLVTYPGNYDQYLQEKEEALRVEELQNAEFDLKLAQEEVWIRQGIKA
                     RRTRNEGRVRALKAMRRERGERREVMGTAKMQVEEASRSGKIVFEMEDVCYQVNGKQL
                     VKDFSAQVLRGDKIALIGPNGCGKTTLLKLMLGQLQADSGRIHVGTKLEVAYFDQHRA
                     ELDPDKTVMDNLAEGKQEVMVNGKPRHVLGYLQDFLFHPKRAMTPVRAFSGGERNRLL
                     LARLFLKPSNLLILDEPTNDLDVETLELLEELIDSYQGTVLLVSHDRQFVDNTVTECW
                     IFEGGGKIGRYVGGYHDARGQQEQYVALKQPAVKKSEEAAAPKAETVKRSSSKLSYKL
                     QRELEQLPQLLEDLEAKLEALQTQVADASFFSQPHEQTQKVLADMAAAEQELEQAFER
                     WEYLEALKNGG"
     gene            12865..14118
                     /gene="pqiA"
                     /locus_tag="OO357_002542"
     CDS             12865..14118
                     /gene="pqiA"
                     /locus_tag="OO357_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiA"
                     /protein_id="HCR5887450.1"
                     /translation="MCEHHHAAKHILCSQCDMLVALPRLEHGQKAACPRCGTTLTVAW
                     DAPRQRPTAYALAALFMLLLSNLFPFVNMNVAGVTSEITLLEIPGVLFSEDYASLGTF
                     FLLFVQLVPAFCLITILLLVNRAELPVRLKEQLARVLFQLKTWGMAEIFLAGVLVSFV
                     KLMAYGSIGVGSSFLPWCLFCVLQLRAFQCVDRRWLWDDIAPMPELRQPLKPGVTGIR
                     QGLRSCSCCTAILPADEPVCPRCSTKGYVRRRNSLQWTLALLVTSIMLYLPANILPIM
                     VTDLLGSKMPSTILAGVILLWSEGSYPVAAVIFLASIMVPTLKMIAIAWLCWDAKGHG
                     KRDSERMHLIYEVVEFVGRWSMIDVFVIAVLSALVRMGGLMSIYPAMGALMFALVVIM
                     TMFSAMTFDPRLSWDRQPESEHEES"
     gene            14123..15763
                     /gene="pqiB"
                     /locus_tag="OO357_002543"
     CDS             14123..15763
                     /gene="pqiB"
                     /locus_tag="OO357_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intermembrane transport protein PqiB"
                     /protein_id="HCR5887451.1"
                     /translation="MESNNGEAKIQKVKNWSPVWIFPIVTALIGAWVLFYHYSHQGPE
                     VTLITANAEGIEGGKTTIKSRSVDVGVVESATLADDLTHVEIKARLNSGMEKLLHKDT
                     VFWVVKPQIGREGISGLGTLLSGVYIELQPGAKGSKMDKYDLLDSPPLAPPDAKGIRV
                     ILDSKKAGQLSPGDPVLFRGYRVGSVETSTFDTQKRNISYQLFINAPYDRLVTSNVRF
                     WKDSGIAVDLTSAGMRVEMGSLTTLLSGGVSFDVPEGLDLGQPVAPKTAFVLYDDQKS
                     IQDSLYTDHIDYLMFFKDSVRGLQPGAPVEFRGIRLGTVSKVPFFAPNMRQTFNDDYR
                     IPVLIRIEPERLKMQLGENADVVEHLGELLKRGLRGSLKTGNLVTGALYVDLDFYPNT
                     PAITGIREFNGYQIIPTVSGGLAQIQQRLMEALDKINKLPLNPMIEQATSTLSESQRT
                     MKNLQTTLDSMNKILASQSMQQLPTDMQSTLRELNRSMQGFQPGSAAYNKMVADMQRL
                     DQVLRELQPVLKTLNEKSNALVFEAKDKKDPEPKRAKQ"
     gene            15760..16323
                     /gene="pqiC"
                     /locus_tag="OO357_002544"
     CDS             15760..16323
                     /gene="pqiC"
                     /locus_tag="OO357_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity-associated transporter
                     subunit PqiC"
                     /protein_id="HCR5887452.1"
                     /translation="MKKWLVTIAALWLAGCSSGEINKNYYQLPVVQSGTQSTASQGNR
                     LLWVEQVTVPDYLAGNGVVYQTSDVKYVIANNNLWASPLDQQLRNTLVANLSTQLPGW
                     VVASQPLGSAQDTLNVTVTEFNGRYDGKVIVSGEWLLNHQGQLIKRPFRLEGVQTQDG
                     YDEMVKVLAGVWSQEAASIAQEIKRLP"
     gene            16579..16746
                     /gene="rmf"
                     /locus_tag="OO357_002545"
     CDS             16579..16746
                     /gene="rmf"
                     /locus_tag="OO357_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome modulation factor"
                     /protein_id="HCR5887453.1"
                     /translation="MKRQKRDRLERAHQRGYQAGIAGRSKEMCPYQTLSQRSQWLGGW
                     REAMADRVVMA"
     gene            complement(16816..17334)
                     /gene="fabA"
                     /locus_tag="OO357_002546"
     CDS             complement(16816..17334)
                     /gene="fabA"
                     /locus_tag="OO357_002546"
                     /EC_number="4.2.1.59"
                     /EC_number="5.3.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014915875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-hydroxydecanoyl-ACP
                     dehydratase/trans-2-decenoyl-ACP isomerase"
                     /protein_id="HCR5887454.1"
                     /translation="MVDKRESYTKEDLLASGHGELFGAKGPQLPAPNMLMIDRVVKMT
                     ETGGNFDKGYVEAELDINPDLWFFGCHFIGDPVMPGCLGLDAMWQLVGFYLGWLGGEG
                     KGRALGVGEVKFTGQVLPTAKKVTYRIHFKRIVNRRLIMGLADGEVLVDCRLIYTASD
                     LKVGLFQDTSAF"
     gene            complement(17403..19163)
                     /locus_tag="OO357_002547"
     CDS             complement(17403..19163)
                     /locus_tag="OO357_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lon protease family protein"
                     /protein_id="HCR5887455.1"
                     /translation="MTITKLAWRDLVPDTDSYQEIFAQPHLIDENDPLFSDTQPRLQF
                     ALEQLLHTRASSSFMLAKAPEESEYLNLIADAARTLQSDAGQLVGDHYEVSGHSIRLR
                     HAVSADDNFATLTQVVAADWVEAEQLFGCLRQFNGDITLQPGLVHQANGGILIISLRT
                     LLAQPLLWMRLKNIVNRERFDWVAFDESRPLPVSVPSMPLKLKVILVGERESLADFQE
                     MEPELSEQAIYSEFEDTLQIVDAESVSQWCRWVTFTARHNHLPAPGADAWPVLIREAA
                     RYTGEQETLPLSPQWILRQCKEVASLCDGDTFSGEQLNLMLQQREWREGFLAERMQDE
                     ILQEQILIETEGERIGQINALSVIEFPGHPRAFGEPSRISCVVHIGDGEFTDIERKAE
                     LGGNIHAKGMMIMQAFLMSELQLEQQIPFSASLTFEQSYSEVDGDSASMAELCALISA
                     LADVPVNQSIAITGSVDQFGRVQPVGGLNEKIEGFFAICQQRELTGKQGVIIPTANVR
                     HLSLHSELVKAVEEGKFTIWAVDDVTDALPLLLNLVWDGEGQTTLMQTIQERIAQASQ
                     QEGRHRFPWPLRWLNWFIPN"
     gene            19348..19800
                     /gene="matP"
                     /locus_tag="OO357_002548"
     CDS             19348..19800
                     /gene="matP"
                     /locus_tag="OO357_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="macrodomain Ter protein MatP"
                     /protein_id="HCR5887456.1"
                     /translation="MKYQQLENLESGWKWKYLVKKHREGELITRYIEASAAQEAVDVL
                     LSLENEPVLVNGWIDKHMNPELVNRMKQTIRARRKRHFNAEHQHTRKKSIDLEFIVWQ
                     RLAGLAQRRGKTLSETIVQLIEDAENKEKYANKMSSLKQDLQALLGKE"
     gene            complement(19876..20922)
                     /gene="ompA"
                     /locus_tag="OO357_002549"
     CDS             complement(19876..20922)
                     /gene="ompA"
                     /locus_tag="OO357_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpA"
                     /protein_id="HCR5887457.1"
                     /translation="MKKTAIAIAVALAGFATVAQAAPKDNTWYTGAKLGWSQYHDTGF
                     IPNNGPTHENQLGAGAFGGYQVNPYVGFEMGYDWLGRMPYKGDNINGAYKAQGVQLTA
                     KLGYPITDDLDIYTRLGGMVWRADTKANVPGGASFKDHDTGVSPVFAGGVEYAITPEI
                     ATRLEYQWTNNIGDANTIGTRPDNGLLSLGVSYRFGQGEAAPVVAPAPAPEVQTKHFT
                     LKSDVLFNFNKATLKPEGQAALDQLYSQLSNLDPKDGSVVVLGYTDRIGSDAYNQGLS
                     ERRAQSVVDYLISKGIPADKISARGMGESNPVTGNTCDNVKQRAALIDCLAPDRRVEI
                     EVKGIKDVVTQPQA"
     gene            complement(21278..21787)
                     /gene="sulA"
                     /locus_tag="OO357_002550"
     CDS             complement(21278..21787)
                     /gene="sulA"
                     /locus_tag="OO357_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division inhibitor SulA"
                     /protein_id="HCR5887458.1"
                     /translation="MYTSGYAHRSSSFSSAASKIARVSTENTTAGLISEVVYREDQPM
                     MTQLLLLPLLQQLGQQSRWQLWLTPQQKLSREWVQASGLPLTKVMQISQLSPCHTVES
                     MVRALRTGNYSVVIGWLTDDLTEEEHAELVDAANEGNAMGFIMRPVSASSHTTRQLSG
                     LKIHSNLYH"
     gene            22006..>22413
                     /gene="sxy"
                     /locus_tag="OO357_002551"
     CDS             22006..>22413
                     /gene="sxy"
                     /locus_tag="OO357_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRP-S regulon transcriptional coactivator Sxy"
                     /protein_id="HCR5887459.1"
                     /translation="MKSLSYKRIYKSQEYLATLGTIEYRSLFGSYSLTVDDTVFAMVS
                     DGELYLRACEQSAQYCVKHPPVWLTYKKCGRSVTLNYYRVDESLWRNQLKLVRLSKYS
                     LDAALKEKSTRNTRERLKDLPNMSFHLEAILGEV"
BASE COUNT         5721 a         5257 c         5874 g         5561 t
ORIGIN      
        1 gtatcgaacc atcaatgact acggggcgga aacgccgcaa ctcaactgta aatcgtaagc
       61 cgtcttcagt taagagagcg agatgtatag aactcatcga caacacagcc tgttaagctc
      121 tggtggagtg ccatcgttta ttggtgggct ggtggtgttt gtgtcggcag cgttcaatgc
      181 acaagctgaa acctggttcg atcctgcttt tttcaaagat gatccctcaa tggtggccga
      241 tttgtctcgt ttcgaaaaag gacaaaaaat aacgccaggg gtttatcgtg tcgatattgt
      301 tctgaatcag acaattgtag atacgcgcaa cgtcaatttt gttgagataa cgccagagaa
      361 ggggattgcc gcctgtttga cgactgaaag cctggatgca atgggtgtta atactgatgc
      421 gtttccggct tttaaacaac tggacaaaca agtgtgtgtg ccattggcgg agattattcc
      481 ggatgccagc gtaacgttta atgtgaataa actccgtctg gaaatttcag taccgcaaat
      541 cgccatcaaa agtaatgccc gtggttatgt cccccctgaa cgttgggatg aagggatcaa
      601 cgcgctatta ctgggatatt cgtttagcgg ggttaacagt attcatagca gcgcagacag
      661 tgattctggc gacagctatt ttctgaattt aaacagtggc gttaatttag gcccatggag
      721 attacgcaac aattcaacat ggagtcgcag tagtggccaa accgcagaat ggaagaatct
      781 cagcagctat ttgcagcggg cggttattcc actaaaaggc gaactgaccg taggtgatga
      841 ttatactgca ggcgattttt tcgatagtgt cagctttcgt ggtgtgcagc tggcgtcaga
      901 tgacaacatg ctgccagaca gcctgaaagg gtttgcgcct gtggtgcgtg gtatcgccaa
      961 aagcaatgcc cagataacga ttaagcaaaa tggttacacc atttaccaaa cttatgtatc
     1021 gcctggtgct tttgaaatta gtgatctcta ttccacgtcg tcgagcggtg atttgttagt
     1081 tgaaatcaaa gaagcggacg gcagcgtcaa tagctacagc gtaccgtttt ccagcgtgcc
     1141 attactccag cgtcaggggc gaatcaaata cgcggtgaca ctggcgaaat acagaaccaa
     1201 tagtaatgaa cagcaggaga gcaaatttgc ccagaccacg ttgcagtggg gcggaccgtg
     1261 gggaacgaca tggtatggtg gtggacaata tgctgaatat taccgtgccg ccatgtttgg
     1321 tctgggattt aaccttggcg atttcggagc aatttcgttc gatgcgaccc aggcgaagag
     1381 tacgctggca gaccaaagcg aacataaagg tcagtcatat cgttttctgt atgccaaaac
     1441 gctcaaccaa ttgggcacta attttcaatt gatgggctat cgctattcga cgtcgggttt
     1501 ctacaccctt tccgacacca tgtataaaca tatggatggc tacgaattta atgacggtga
     1561 tgatgaagat acgccgatgt ggtcgcgtta ttacaatttg ttttacacca aacgtggcaa
     1621 actgcaggtc aatatctccc agcaattagg cgagtacggt tcgttttatt taagtggtag
     1681 ccagcaaact tactggcata ccgatcaaca ggatcggcta ttacagtttg gctacaacac
     1741 gcaaattaaa gatctctcgc tgggggtttc ctggaactac agtaagtccc gtggtcaacc
     1801 tgatgctgat caggtgtttg cactaaattt ttccctgccg ctcaatctgt tgctccccag
     1861 aagtaatgat agctatacca ggaaaaaaaa ttacgcctgg atgacctcta acaccagtat
     1921 cgataacgaa gggcacatta cacaaaacct gggtttaacg gagacactac tcgatgacgg
     1981 taacctgagc tacagcgtgc aacagggata taacagcgag gggaaaacgg ctaatggtag
     2041 cgccagcatg gactacaaag gggcgtttgc agatgcccga gtgggctaca actacagcga
     2101 taacggcagt caacaacaac tgaactacgc tctttcaggc agtttagttg cccattcaca
     2161 gggcattacc ctggggcaat cgctggggga aactaacgtg ctgattgcag caccaggcgc
     2221 agagaatact cgtgtggcga acagcaccgg gctgaaaact gactggcgcg gatataccgt
     2281 tgtcccctat gccacttctt atcgggaaaa tcgaatcgca cttgatgcgg catcgttaaa
     2341 acgtaacgtg gatcttgaaa atgcagtagt caacgtggtt cccaccaaag gggcgttggt
     2401 tctggcggag ttcaatgccc atgcgggagc aagggtatta atgaaaacat caaagcaggg
     2461 tataccgctg cgttttggcg cgatagcgac gctggacggc atacagacta atagcggcat
     2521 tattgatgat gatggctcgc tctatatgtc cggtttgccc gctcagggag cgataactgt
     2581 acgctggggc gaagctcccg atcaaatttg tcatatcagt taccagctta ccgaacaaca
     2641 aattaactct gcgattacgc gaatggatgc catatgcaga taatctttgg agaaaaatgc
     2701 gtgttattac tacgactatt ttttgccgcc gtcttaatgc tatggtgcgc tcaaactgct
     2761 gcttatatcg ggcagtgtca taccactcag gggaatccgt atattggcgt caattttggc
     2821 gttaaaactc tggatgaaga agaaaatacg gctggagtag ttaaagacaa attttatcag
     2881 tggaacgaat cgaatgatta ttatgtttcc tgtgattgcg ataaagacaa tgtcagaagt
     2941 ggccgatggg cattcgccgc ggattcaccg ttagtctatt taggcgacaa ctggtacaaa
     3001 attaatgact atcttgccgc caaagtttta ttgcaggtta aaggcagttc tcctactgcg
     3061 gttcctttcg aaaacgtggg cacaggggca gatacacgat ggcatatttg cgatcccggc
     3121 ggtcaacgtt taggtggcca gggggctagc ggtaatagcg gtagcttttc cctgaaaata
     3181 ttgcagccgt tcgttggttc ggtcgtcatt cctcctatgg cgctggcgcg attatttgaa
     3241 tgctacaaca tacccgcagg tgattcctgc acgactactg gtacaccggt tttagtgtat
     3301 tacctgtctg gtacgatcaa ttcacttggc tcatgttccg tcaatgccgg agaaacaatc
     3361 gaggttgatc tgggcgacgt atttgcggct aactttcgtg ttgtagggca taagcctctt
     3421 ggggccagaa cggcagaact tgcaattccg gtcaggtgta acacgggaaa cgcagggtta
     3481 gttaacgtca atctgagtct gacggcaacc tcagacccca gctatcccca ggcgattaag
     3541 acgtcacgtc ctggcgtggg cgtggtggtg accgatagcc agcacaacat tatttcccct
     3601 gctggtggga cactaccgct ctctattcct gatgatgcag acagtatccg cgaatgaatg
     3661 tctatccagt cagcacgaca agcgtaccac cagaaaccgg gcgatttgaa gccacggcaa
     3721 cggtgagaat aaattttgat taacgcgtga acgtatgaag aaaaaaacga tatatcagtg
     3781 cgttattttg ttctttagcc ttcttaacat ccatgtcggg atggctgggc ctgaacaagt
     3841 cagtatgcat atttatggga atgtggtcga tcagggctgt gatgtcgcca ccaaaagtgc
     3901 attacaaaat attcatattg gtgattttaa tatcagtgat tttcaggccg cgaataccgt
     3961 aagcactgct gctgatttga atattgatat caccggttgt gccgctggta ttactggcgc
     4021 ggacgtcctt tttagcggcg aggctgacac ccttgcgccg acactgctca aactaactga
     4081 cacaggcgga agcggtggta tggcaacggg gattgccgtg cattcttgat gcgcaaagtc
     4141 agcaagaaat cccgctcaat caggtccagc ctcttacgcc cttaaaagcc ggggataaca
     4201 cactcaaata tcaacttcgt tataagtcca caaaggcggg agcaacgggc ggtaatgcga
     4261 cggcggttct ctattttgat ctggtttacc agtgaaggag tgatgttgaa acggataatc
     4321 tggattctgt tcttactggg attaacgggg ggctgtgagc tatttgccca tgatggcacg
     4381 gtcaacatta gcggatcgtt tcgccgtaat acatgcgtgc tggcacagga tagcaagcaa
     4441 atcaatgtgc agttaggcga tgtctcgctg acacgttttt ttcatgggaa ttatggcccg
     4501 gagaaatctt tcatcattaa tttgcaggat tgcggaacag atgtcagcac ggttgatgtc
     4561 accttttcag gaacccccga cggagtgcag agcgaaatgc tgtccattga aagtggtacg
     4621 gatgcagcca gtgggctggc aattgcgatt ctggatgatg cgaagatatt gattccgctt
     4681 aaccaggcga gtaaagacta cagcctacat agcggtaagg tgccattaac tttttatgcg
     4741 caattgcgac ctgtcaatag cgatgtgcag tcgggtaaag tgaatgccag cgcgacattt
     4801 gtacttcatt atgactaata cgtggaacag attagcgctc ttgattttcg ccgttttatc
     4861 gctgctggtg gcgggtgaat tgcaggcagg agtggtggtc ggcggaacgc gatttatctt
     4921 tccggcagac agagaatcga tatctatttt actgactaat accagtcagg aatcttggct
     4981 tattaacagc aaaatcaatc gcccaacgcg ttgggccggg ggggaagcgt cgacagtgcc
     5041 agcactatta ctggccgctc cgccacttat tctcctgaag cccggtacta caggcacgtt
     5101 gcgcttgctg agaacggaaa gcgacatctt gcctgtggat cgcgaaacgc tatttgagtt
     5161 aagcattgcc agcgtgccat ccggcaaagt tgaaaatcag agcgtaaaag tggcgatgcg
     5221 ctcggtattt aaactgttct ggcgacccga aggtttgccg ggcgacccgc tggaagctta
     5281 tcaacaatta cgctggacac ggaattcgca gggtgtacaa ctcactaacc caacgcctta
     5341 ctacattaac ctgattcagg tgagcgtaaa tggtaaagcg ttaagtaatg cgggagtcgt
     5401 gccgcctaaa agccagcgtc agacaaactg gtgtcaggta atagcaccgt gtcatgtcgc
     5461 ctggcggacg ataaatgatt acggcgggtt gagtgcaaag aaggagcaaa atctgccctg
     5521 aaacaggttc tgaaaacgtt tgcgtttttt ttgccgcagg tcaattccct tttggtccga
     5581 actcgcacat aatacgcccc cggtttgcac accgggaatc caggagagtt catgtactac
     5641 cccttcgttc gtaaagccct tttccagctc gatccagagc gcgctcatga gtttactttt
     5701 cagcaattac gccgcattac cggaacaccg tttgaagcac tggtgcggca gaaagtgcct
     5761 gcgaaacctg ttaactgcat gggcctgacg tttaaaaatc cgcttggtct ggcagccggt
     5821 cttgataaag acggggagtg cattgatgcg ttaggcgcga tgggatttgg atcgatcgag
     5881 atcggtaccg tcacgccacg tccacagcct ggtaatgaca agccgcgtct ctttcgtctg
     5941 gtagatgccg aaggtttgat caaccgtatg ggctttaata atcttggcgt tgataacctc
     6001 gtagagaacg taaaaaaggc ccattatgac ggcgtcttgg gtattaatat cggcaaaaat
     6061 aaagatacgc cagtggagca gggcaaagat gactatctga tttgtatgga aaaaatctat
     6121 gcctatgcgg gatatatcgc catcaatatt tcatcgccga ataccccagg attacgcacg
     6181 ctgcaatatg gtgaagcgct ggatgatctc ttaaccgcga ttaaaaataa gcaaaatgat
     6241 ttgcaagcga tgcaccataa atatgtgccg atcgcagtga agatcgcgcc ggatctttct
     6301 gaagacgaat tgatccaggt tgccgatagt ttagttcgcc ataatattga tggcgttatt
     6361 gcaaccaata ccactctcga tcgttctctg gttcagggaa tgaaaaattg cgatcaaacc
     6421 ggtggcttaa gtggtcgtcc gcttcagtta aaaagcacag aaattattcg ccgcttgtca
     6481 ctagaattaa acggtcgctt accgatcatc ggtgttggcg gcatcgactc ggttatcgct
     6541 gcgcgtgaaa agattgctgc gggtgcctca ctggtgcaaa tttattctgg ttttattttt
     6601 aaaggtccgc cgctgattaa agaaatcgtt acccatatct aattatttct tcgaattcct
     6661 ctttataaga taatcagggc tttattttca gacctggttg ttttatattc atcgttgttg
     6721 cttatttaga cattttgtac ttttattgtt gaggttatta agcgaagcga caatggattg
     6781 tgttgctgcg gttttatagg gtaggggaga ggcagatgcg aattaaacca gacgataact
     6841 ggcgttggta ttacgatgaa gagcacgatc gaatgatgct cgatttagcc aatggtatgc
     6901 tatttcgctc acgttttgcg cgcaaaatgt tgactccaga tgccttttct cccgcaggct
     6961 tttgtgttga tgatgccgcg ctctatttct cttttgaaga aaagtgccgc gattttaatt
     7021 tatctaaaga ccaaaaagcc gaactggtgc tgaatgcact ggtagcaatt cgttatctca
     7081 aaccgcaaat gccgaaaagc tggcattttg tctcccatgg tgaaatgtgg gtgcccatgc
     7141 cgggcgatgc ggcctgcgtt tggttgagcg atactcacga gcaggtcaat ttgctggtcg
     7201 ttgaatctgg cgaaaatgcc gcgctatgcc tgctggcaca accctgcgtt gttattgcgg
     7261 gtcgcgcgat gcagttgggc gacgccatta aaatcatgaa cgacaggctg aaaccgcagg
     7321 tgaatgttga cagcttcagc ctcgaacagg cagtctaacg cgtcagctta agggcagtct
     7381 tcggtacaca gctacagcaa agaatggtgc catcatcgcc cattgctgat tttttcagcg
     7441 gtgtgacttc gccttctaaa agctgaacac ggcaacttcc acaaatgccc gcgcggcaag
     7501 aataagggat acgaattccc tgattttcta attgctccag caacacttgt tggttatttc
     7561 cacgaaatgc ctgtccctgc cagtcaatat ctacatttgc gtccggttgt tgcgtgatgt
     7621 tggcggtatc atcagcggca gctgcgccgt aaatttttgc cggagccgtt gccagaattt
     7681 ccacctcatc gccaacgcga atcacgccgc tattacgggc aattaaattc tgaccaaaat
     7741 cgacatcgcc gttatcctgg gcagtgcgga aagattgcaa tgtttttaat ggttcgcctg
     7801 ccggatgttt ttgccctttt tctgggctga cggtggtgaa aatacagcgg ctacaaggtt
     7861 taaccacatc aaacaccaca tcaccaatgc gaatcacttt ccagctatct tcttcccagg
     7921 ctgacgcgcc ggaaaccacc agattggggc ggaattgctc catttttaca ctggccggac
     7981 aacgttgttg gagatcacgt aacgaggctt cgttagcaag aaggtaagga tagccatcag
     8041 caaatgacag aggtacagtg ttgtggcgtt tcacgcgccg ggtcatttgt ggccccaccc
     8101 agcgtaattg cacttcgcgg gagaaaaatc cacttagcca tttgttgatc gcgtctggcg
     8161 caattcgcgc ggtaaaatgt gtgccccaaa cttcggttgg tgcgtcttgt gtggcgaaat
     8221 cagcaaaacg aacatatgcg ctactgccat ctggtgcggt gagatgcaag ccatcatgca
     8281 caggtgaagg agtaaaccgt accatctggg gaaactggcg agcggtaata aacgtaccgt
     8341 caggttccgt gatcataaag atgcgatcga aggccagacc actgacatca gccagagcat
     8401 gtgtaagacc aatgccgcgc atcgatttaa caggatgaat aaaaagccgg gttaatgtcg
     8461 ccacggtgcg gtcctcaaat gaaaataagc cctcaacttt atgacatgac gcgcttatta
     8521 gctataatgc gcaacaattt tcttagcgcc tgtcccgtta ggctatttta tttgtcagtt
     8581 tggccctggg ttgtgctcga aatcctcacg tacttgtgta cgctccggtt tctccgcgct
     8641 ctccatgtcc agactgactg caacaattac gcctactgcg gtaggctctt agagtaaaag
     8701 tgacgatatg aattctctgt ttgccagtac ggcccgtggg ctggaagagc tgttaaaaac
     8761 tgaactggaa aatctggggg ccgttgaatg ccaggtggtt cagggtgggg tccatttcaa
     8821 gggcgacaca cggcttgttt accagagcct gatgtggagc cgcctggcct cgcgtattat
     8881 gttgccgctg ggcgagtgta aggtttacag cgatttagac ctctatctcg gtgttcaggc
     8941 gatcaactgg acagagatgt ttaatcctgg cgcgaccttc gctgtccact tcagtggttt
     9001 gaatgacacc atccgcaaca gtcagtacgg cgcgatgaaa gtgaaagacg cgatcgtcga
     9061 tgctttcaca cggaaaaatc tgccgcgtcc aaatgttgat cgcgatgcgc cggatatccg
     9121 cgttaacgtc tggctgcata aagaaaccgc cagtatcgct cttgatctga gtggtgatgg
     9181 tttacatctg cgtggctatc gcgatcgtgc tggtattgcg ccgatcaaag aaaccctggc
     9241 agccgctatt gtgatgcgat ccggctggca gtcaggaaca ccgctgctcg atccgatgtg
     9301 tggttccggt acgttgctga ttgaagcggc gatgctggcg accgatcgcg caccaggctt
     9361 gcaccgtggg cgttggggct ttagcggctg ggcgcagcat gatgaagcta tctggcagga
     9421 agtgaaagcg gaagcgcaaa ctcgcgcccg taaaggccta gctgagtata gctctcattt
     9481 ttacggttcg gacagcgacg cacgggtgat tcaacgtgcg cgcactaacg cccgtcttgc
     9541 ggggattggt gaactgatca cctttgaggt gaaagatgtc gcgcaactga ccaatccgct
     9601 gccgaaaggg ccgtacggta cagtgttgag caacccgcca tacggtgaac gtctggacag
     9661 cgaaccggcg ctgattgcgc tgcatagtct gctgggccga atcatgaaaa accagttcgg
     9721 tggctggaat ctctctttgt ttagtgcctc gccggatctg ctaagctgcc tgcagctgcg
     9781 tgcagacaaa cagtacaagg cgaaaaacgg cccgctggac tgcgtacaga aaaattacca
     9841 cgttgccgaa agcacaccag acagtaaacc ggcaatggta gcggaagatt acgccaaccg
     9901 cctgcgtaag aacctcaaaa aattcgagaa gtgggcacgc caggaaggga ttgaatgtta
     9961 ccgcctgtat gacgccgatc tgccggaata taacgttgcc gttgaccgtt atgccgactg
    10021 ggtggtggtg caggagtatg cgccgccaaa aactattgat gcgcacaaag cgcgtcagcg
    10081 tctgttcgat attatcgctg caaccatttc ggtactgggg attgcgccaa acaaactggt
    10141 gctgaaaacc cgtgaacgcc ataagggcaa aaaccagtac cagaaactgg gcgagaaggg
    10201 cgagtttctt gaagttaccg aatataacgc tcacttgtgg gtgaacctga cggattatct
    10261 cgatactggt ctgttcctcg atcaccgtat cgcccgccgt atgctcggtc agatgagcaa
    10321 aggcaaagat ttcctcaacc tgttttctta taccggcagc gccaccgtgc atgcgggatt
    10381 aggcggtgca cgcagcacca ccaccgtgga tatgtcgcgt acttatctgg aatgggcaga
    10441 acgcaacctg cgtctgaatg gcctgactag gcgtgcgcat cgcctgattc aggccgattg
    10501 cctggcgtgg ctgcgtgagg caaatgaaca gttcgatctg atctttatcg atccgccaac
    10561 cttctctaac tcaaaacgaa tggaagatgc gtttgatgtt cagcgcgatc atctggcgct
    10621 gatgaaagat ttgaaacgtc tgctgcgtgc aggtgggacg atcatgttct cgaacaacaa
    10681 acgtggcttc cgtatggatc tcgacggcct ggcaaaactg ggactgaaag cacaagaaat
    10741 tacgcaaaaa acgctctccc aggatttcgc ccgtaaccgc cagatccaca actgctggct
    10801 gattaccgca gcctgaaagg aatagtaatg tcattaatca gtatgcatgg cgcatggctg
    10861 tcgttcagcg acgcgccgct tctcgataac gcagaactgc atatcgaaga taacgaacgt
    10921 gtttgtctgg tgggccgcaa cggcgcagga aaatcgacgt taatgaaaat cctcaaccgt
    10981 gaacaagggc tggatgacgg tcgcattatt tatgagcaag atttgattgt agctcgtctg
    11041 caacaggatc cgccgcgtaa cgttgagggt agcgtttatg atttcgttgc cgaaggcatt
    11101 gaagaacaag cggaatatct gaaacgctat cacgatattt cgcgcctggt gatgaacgac
    11161 ccgagcgaga aaaatctcaa cgaactggcg aaggttcagg aacagctgga tcaccacaac
    11221 ctgtggcagc tggaaaaccg catcaacgaa gtgctggcgc aactggggtt agatcctaac
    11281 gttgcgctgt cgtcgctttc cggcggctgg ttgcgtaaag cggcattagg acgcgcgctg
    11341 gtgagcaatc cgcgcgtatt gctgctggac gaaccgacta accatctgga tatcgaaacc
    11401 atcgactggc tggaggggtt ccttaaaacc ttcaatggga cgattatttt catctcccac
    11461 gaccgttcgt ttatccgcaa tatggcgacg cgcattgttg atctcgatcg cggcaagctg
    11521 gtgacctatc cagggaatta cgaccagtac ctgcaggaaa aagaagaagc cctgcgcgtg
    11581 gaagaactac aaaatgccga gttcgatctc aaactggcgc aggaagaagt gtggattcgc
    11641 caggggatca aagcacgccg tacccgtaat gaaggccgcg tacgcgccct gaaagcgatg
    11701 cgtcgcgaac gtggtgaacg tcgcgaagtg atgggtaccg caaagatgca ggtggaagag
    11761 gccagccgct ccggtaagat cgttttcgaa atggaagacg tttgctacca ggttaacggt
    11821 aagcaactgg tgaaagattt ttctgcccag gttctacgtg gcgacaaaat tgccctgatt
    11881 ggtccgaatg ggtgcggcaa aaccacgctg ctgaaactga tgctcggtca gcttcaagcg
    11941 gacagcgggc gtattcacgt tggcaccaaa ctggaagtgg cttatttcga tcagcaccgc
    12001 gcggaactgg atcccgataa aacggtgatg gataaccttg ccgaaggtaa gcaagaggtg
    12061 atggttaatg gcaagccacg ccacgtattg ggctatttgc aggactttct gttccatccg
    12121 aaacgggcga tgacgccagt acgtgcgttt tctggcggtg agcggaaccg cttgctgctg
    12181 gcgcgtttgt tcctcaaacc aagcaactta ttgattcttg acgaaccgac caacgatctt
    12241 gatgtcgaaa cgctggaact gctggaagaa ctgatcgaca gctatcaggg cacggtattg
    12301 ctggttagcc acgatcgtca gtttgtcgat aacaccgtta cagaatgctg gatcttcgaa
    12361 ggcggcggta aaattggtcg ttatgttggc ggttatcatg atgcccgtgg tcagcaagaa
    12421 caatatgtgg cactcaaaca gcctgcggtg aaaaaaagcg aagaagccgc cgcgccaaaa
    12481 gcagaaactg taaaacgcag cagtagcaaa ctaagctata aattgcagcg cgaactggag
    12541 cagctaccgc aattgctcga agatctggag gcgaagctgg aagccctaca gacgcaagtg
    12601 gcggatgctt ccttcttcag tcagccgcat gagcagacgc aaaaagtgct tgctgatatg
    12661 gctgctgcag agcaggagct ggagcaagcc tttgaacgct gggagtatct tgaagcgtta
    12721 aaaaatggtg gctgatcgca aaatagtcga tttaaacaga gacagctgac aacggtaaaa
    12781 ttgcctgatg aactgggttt atcaggcatg aaaaccaaca ctttgttagt catctgtaaa
    12841 atagcgcatc attaaggagt accaatgtgc gaacatcatc atgccgcgaa gcacatcctg
    12901 tgctcgcagt gtgacatgct ggtggcgtta ccgcgccttg agcatggtca gaaagcggca
    12961 tgtccccggt gtggcacaac gttaaccgtg gcgtgggatg cccctcggca gcgtcccacc
    13021 gcctatgcgt tggctgcact gttcatgctg ttgctgtcca acttgtttcc ttttgtgaat
    13081 atgaacgttg caggagttac cagtgaaatt acattactgg aaattcccgg cgtgcttttt
    13141 tctgaggact acgccagcct cggcaccttt ttcctgttgt ttgtgcaact ggttcccgcg
    13201 ttttgtctga taaccattct gttactggtg aatcgcgcgg aattaccggt ccgtttaaaa
    13261 gagcaactgg cacgggtgct ttttcaactc aaaacctggg gaatggcgga gattttcctc
    13321 gcgggtgtgc tggtcagttt cgttaaactg atggcttacg gcagcattgg ggtaggcagc
    13381 agctttctcc cctggtgttt attttgtgtc ctgcaactgc gcgcttttca gtgcgttgat
    13441 cgtcgctggt tatgggacga catcgccccg atgccagaac tgcgccagcc gctaaaacca
    13501 ggcgtcacgg ggatacgtca ggggctgcgt tcgtgctcct gttgtacggc aatccttcct
    13561 gctgatgaac ccgtgtgccc gcgttgtagt accaaagggt acgttcggcg tagaaacagc
    13621 ctgcagtgga cactcgcgct gcttgtaacg tccatcatgc tgtatcttcc ggctaatatt
    13681 ttgcccatca tggtgacgga tttattaggc tcgaagatgc cgtcgacgat tctcgctggg
    13741 gtcattctgt tatggagcga aggctcttat cccgtcgctg cggtgatctt tctggccagt
    13801 attatggtgc caacgttaaa gatgatcgcc atcgcgtggc tgtgttggga tgccaaaggg
    13861 catggcaagc gcgacagtga aagaatgcat ttgatttatg aagttgttga gtttgtaggc
    13921 cgctggtcga tgattgacgt tttcgttatc gcggtgctct cggcgctggt gcgtatggga
    13981 ggtttaatga gtatttatcc ggcaatgggt gcattaatgt ttgctttagt cgtcataatg
    14041 acaatgtttt ctgctatgac gtttgacccg cgtttgtcgt gggatcgtca acctgaatca
    14101 gagcatgagg agtcctgaca gtatggaatc taataatggg gaagccaaaa tccagaaagt
    14161 gaagaactgg tctcccgtgt ggatatttcc tatcgtcacg gcgctcattg gggcctgggt
    14221 tcttttttat cattacagcc atcagggacc ggaagtgacc ctgatcaccg cgaatgcgga
    14281 aggaattgaa ggtggcaaaa ccaccattaa aagccgtagc gttgacgtcg gcgtggttga
    14341 aagcgccaca ctggctgatg atttgacgca cgttgaaatc aaagcgcggc tgaattccgg
    14401 tatggaaaaa ttgctgcata aagacaccgt cttttgggtg gtgaaaccgc agattggtcg
    14461 cgaagggatt agcggcctgg gaacgctgct gtctggagtt tatatcgaac tgcagccagg
    14521 cgcgaaaggc agcaaaatgg ataaatacga tttgctggac tcaccaccgt tggccccgcc
    14581 tgatgcgaaa ggtatccgtg tgattctcga tagcaaaaaa gccgggcagc tctcgccagg
    14641 agatccggtg ctgttccgtg gctatcgggt aggttcggtt gaaaccagca ccttcgatac
    14701 gcaaaaacgc aatatcagtt atcaactgtt catcaatgca ccttatgacc gactggtgac
    14761 cagcaatgtt cgcttctgga aagatagtgg cattgcggtt gatctgacgt cagcggggat
    14821 gcgtgtggag atgggctcat tgacaacgct gcttagtggc ggtgttagct ttgatgtgcc
    14881 ggaaggtctg gatttagggc agccagtggc accgaaaaca gctttcgttt tgtatgatga
    14941 tcagaagagc attcaggatt cgttgtacac cgatcacatt gattatctga tgttctttaa
    15001 agattcggta cgcggtctgc aaccgggagc tccggtagaa ttccggggta ttcgcctggg
    15061 taccgtaagc aaagtgccat tctttgcgcc gaatatgcgt cagacattta acgatgatta
    15121 ccgtattccg gtactgattc gtatcgagcc agagcggctg aaaatgcagc ttggcgaaaa
    15181 tgcggatgtt gttgagcacc ttggcgaatt gttgaaacgt ggtttacgcg gatcgctgaa
    15241 aaccggaaac ctggtcactg gtgcactgta tgttgatctc gatttctatc caaatacgcc
    15301 tgcaataacc ggtattcgtg aatttaatgg ttatcagatt atcccgactg ttagcggcgg
    15361 cttggcgcaa atccagcaac gactgatgga agcgttggat aagatcaaca aactgccatt
    15421 gaatccgatg attgaacagg caaccagtac gctttctgaa agtcagcgca caatgaaaaa
    15481 cctgcaaacg acgctggata gcatgaacaa gatcctcgcc agccagtcga tgcagcagtt
    15541 gccgacggat atgcagtcaa cgttgcgtga attgaatcgc agcatgcagg gcttccagcc
    15601 tggctccgca gcctacaaca agatggtggc ggatatgcag cgccttgatc aggtgttgcg
    15661 agaactgcaa ccggtgctga aaacgctcaa tgagaagagt aacgcgctgg tatttgaagc
    15721 gaaggacaaa aaagatccag agccgaagag ggcgaaacaa tgaaaaagtg gctagtgacg
    15781 attgcagcac tgtggctggc cggatgcagc tccggcgaaa ttaataaaaa ctattaccag
    15841 ttacctgtgg tgcagagcgg tacacaaagt accgccagcc agggcaatcg tctgttatgg
    15901 gtagagcagg tcactgttcc tgactatctg gcggggaatg gtgtggttta tcaaaccagt
    15961 gatgtgaagt atgtgattgc caacaacaac ttgtgggcca gcccgttgga tcaacagttg
    16021 cgcaacaccc tggttgccaa cctgagtacg caactgcccg gctgggtggt tgcctcccag
    16081 cctctgggaa gcgcccagga cacgctcaat gttaccgtaa cggagtttaa cggtcgctat
    16141 gatggcaagg tcattgtcag tggtgagtgg ctgttgaacc accagggaca actgatcaaa
    16201 cgtccgttcc gtctggaagg agtgcaaact caggatggtt acgatgagat ggttaaagtg
    16261 ctggccggtg tctggagtca ggaagccgct tctattgcac aagagataaa gcgtctacct
    16321 taattataaa gatttgtaaa tataaccgtc tccggtatgt tgcctgaggc ggtttttttg
    16381 tttctaacgt gcggaaaaat ttgttcctct tcacattttt tgtacaaccg acatgcccgt
    16441 gtagctcaca aatatgacag tggcgtgaat tttgcgcatt gacggcagtt atgattcgcg
    16501 gtattgctta actgtgattg cacatttagt aatcactgtt ttcttttcca ccagaaacca
    16561 gtatgaggga aacgaggcat gaagagacaa aaacgagatc gcctggaacg ggcacatcaa
    16621 cgtggttatc aggccggcat cgccggacgc tcaaaagaaa tgtgtcccta tcagacgctg
    16681 agtcaaaggt cacaatggct gggaggctgg cgagaagcca tggcggacag ggtagtaatg
    16741 gcctgattct gtctctttaa aaagaaacct ccgcattgcg gaggtttcgc cttttgatac
    16801 tctgtctgat gataatcaga aggcagacgt atcctggaac agaccgactt tcaggtcgct
    16861 ggcggtatag atcagacgac aatcaaccag cacttcgcca tctgccagac ccataatcag
    16921 acgacgatta acaatgcgtt taaagtgaat acggtaggtc acttttttcg ctgtcggcag
    16981 tacctgacca gtgaatttca cttcgccaac gcccagcgcg cggcctttac cttcgccgcc
    17041 cagccagccg aggtagaacc ctaccagctg ccacattgcg tccaggccca ggcatcccgg
    17101 cataaccgga tcgccaatga agtggcatcc gaagaaccac agatccggat tgatatccag
    17161 ttctgcttca acataccctt tgtcgaagtt accacccgtt tcggtcattt tgaccacacg
    17221 gtctatcatc agcatgttcg gtgctggcaa ttgcgggcct ttagcgccaa acagttcacc
    17281 gtgaccagag gcaagaaggt cttcttttgt ataggattcg cgtttatcta ccatgttctc
    17341 tgtaagcctt attttattga agcacgcagg atagctaaca cgtgtacgct gaacaagtcc
    17401 gatcagttcg gaataaacca gttcagccaa cgtaatggcc atggaaaacg gtgacgtcct
    17461 tcctgttgcg atgcttgcgc gatacgttcc tggatggttt gcatcagcgt cgtttggcct
    17521 tcgccatccc acaccagatt taataataac ggcagtgcgt cagtcacatc gtctactgcc
    17581 cagatggtga atttgccttc ttctaccgct ttcaccagtt cactgtgaag acttaaatgg
    17641 cgaacgttag cagtggggat aatgacacct tgtttccccg ttaactcacg ttgctggcaa
    17701 atagcaaaga agccttcgat tttctcattt aaaccaccga ccggctggac gcggccgaac
    17761 tgatcgactg aacctgtgat agcgatactc tgattcactg gcacatcagc gagggcgctt
    17821 atcagggcgc agagttcagc catcgaggca ctatcgccat ccacttcact gtatgactgc
    17881 tcaaatgtca gcgatgctga gaaggggatc tgttgctcaa gctgtagttc cgacatcagg
    17941 aacgcctgca tgatcatcat ccctttcgca tggatattgc cgccaagctc cgctttgcgt
    18001 tcgatgtcgg tgaattcacc atcgccaata tgcacaacgc agctaatgcg agaaggttcg
    18061 ccaaaagcgc gtggatgacc cggaaattca atgaccgaaa gggcgttaat ttgcccgatg
    18121 cgttcgcctt cggtttcaat caggatttgc tcctgaagga tctcatcctg catacgttca
    18181 gcgaggaaac cttcgcgcca ttcacgctgc tgcagcatta agtttagctg ctcgccggag
    18241 aaggtgtcgc catcacacag ggaggcgacc tctttacact ggcggaggat ccactgcggg
    18301 ctaagcggaa gtgtttcttg ttcaccggtg tagcgtgctg cttcgcggat aagtaccggc
    18361 caagcatccg ctcccggggc aggtaagtga ttatgtctgg cggtaaatgt cacccagcga
    18421 caccactggc ttactgactc cgcatcgaca atctgcagag tatcttcaaa ttcgctataa
    18481 atagcctgct ctgaaagctc tggctccatc tcctggaaat cagccaatga ttcgcgttcg
    18541 cctaccagaa tgactttcag cttcaatggc attgaaggca cagagacggg gagagggcgc
    18601 gactcatcaa acgcaaccca gtcaaaacgc tcgcggttaa cgatattttt cagccgcatc
    18661 cacagcagag gttgcgccag cagtgtacgc aaagagatga tgagaatacc gccatttgcc
    18721 tgatgcacca gaccaggctg cagggtaatg tcgccattaa actggcgcag gcagccaaag
    18781 agttgctccg cttctaccca gtcggcagcg acaacttgcg ttaaagtcgc aaaattatca
    18841 tctgcactca ctgcgtgacg taagcggatg gagtggccgg aaacctcata gtgatcgccc
    18901 accagttggc ctgcatcgct ttgtagcgta cgcgcggcat cggcaataag attcagatac
    18961 tcagactctt ccggggcctt agccagcata aaagaggagg atgctcgcgt atgcagcaac
    19021 tgctccagcg caaattgcag tcgcggttga gtatcactga ataaaggatc gttttcgtca
    19081 atcaaatgtg gctgagcaaa tatttcctga tagctatcgg tatcaggaac caggtcacgc
    19141 catgcaagtt tcgtaatggt caaagttgat gttttttagt ctgttgtcaa agccgcgatt
    19201 ataccgtaac cggcactaca gcacacgtag aaagcaccga caatactcct ggctgggcgt
    19261 taaagctcac aggatggaga ttcttttctt cactggccta aaaagctgat attctgtaaa
    19321 gagttacacg gtaacattga gatcgctatg aaatatcaac aacttgaaaa tcttgaaagc
    19381 ggttggaaat ggaagtatct ggtgaagaag catcgcgaag gggagttaat tacccgttac
    19441 atagaagcca gtgccgccca ggaagccgtc gatgtgttgc tctcgctcga aaatgaaccc
    19501 gtgctggtaa atggctggat tgacaagcat atgaatccgg aactggtcaa tcgcatgaag
    19561 cagaccattc gggccagacg gaagcgccat tttaatgcgg aacaccagca tacgcgcaaa
    19621 aaatcgatcg atctggaatt tattgtctgg caacgtctgg ctggtcttgc acagcgtcgc
    19681 ggtaaaacgc tttctgaaac gattgttcag ctgattgaag atgcggaaaa caaagagaaa
    19741 tacgcgaata aaatgtcttc tcttaagcag gatctgcagg cattgctggg taaggaataa
    19801 ctgacgaaag tcagttcaat ttactaaagg caaaaaaaac cccgcagcag cggggttttt
    19861 ctaccagacg ataacttaag cctgcggctg agttacaacg tctttgatac ctttaacttc
    19921 gatctctacg cgacgatccg gagccaagca gtcgatcagt gcagcacgct gtttcacgtt
    19981 gtcacaggtg ttgccagtaa ccgggttgga ttcgcccata ccacgtgcgg agatcttgtc
    20041 tgccgggata cctttggaga tcaggtaatc aacaacagac tgagcacggc gctcggacag
    20101 accctggttg taagcgtcag aaccgatgcg gtcagtgtaa cccagaacaa ctacggaacc
    20161 gtctttcgga tctaggttgc tcagctggct gtacagctga tccagagcag cctgaccttc
    20221 cggtttcagg gttgctttgt tgaagttgaa cagaacgtca gacttcagag tgaagtgctt
    20281 ggtctgtact tccggtgccg gagctggagc aactaccgga gctgcttcgc cctgaccgaa
    20341 acggtaggaa acacccaggc tcagcagacc gttgtccgga cgagtaccga tggtgtttgc
    20401 gtcaccgatg ttgttggtcc actggtattc cagacgggta gcgatttcag gagtgatcgc
    20461 gtactcaaca ccacctgcga aaaccggaga aacgccagtg tcgtggtctt taaaggatgc
    20521 gccaccaggt acgttagcct tggtgtctgc acgccatacc ataccaccca gacgagtgta
    20581 gatgtccaga tcgtcagtga ttgggtaacc cagtttagcg gtcagctgaa cgccctgagc
    20641 tttgtatgcg ccgttgatgt tgtcgccttt gtacggcata cgacctaacc agtcgtaacc
    20701 catttcaaaa ccaacatacg ggttaacctg gtaaccacca aaagcacctg cacccagttg
    20761 gttttcgtgg gtcggaccat tgttaggaat aaaaccagtg tcatggtact gggaccagcc
    20821 cagtttagca ccagtgtacc aggtgttatc tttcggagcg gcctgcgcta cggtagcgaa
    20881 accagccagt gccactgcaa tcgcgatagc tgtctttttc attttttgcg cctcgttatc
    20941 atccaaaata cgccatgaat atctccaacg agataacacg gttaaatcct tcaccggggg
    21001 atctgctcaa tattaactct accgatatct tcggcttatg ccgagcaccc ctggcgatgt
    21061 aaagtctaca acgtagttga aaacttacaa gtgtgaactc cgtcaggcat atgaaaaaaa
    21121 agtcttgtat aaggtatgtt taatcttttt gtcagcgaca atttacagaa aaaaaacgcg
    21181 gaaaccgctt cagacaagcc ttcgcaagga aaattagtca cgactgaaag cattggctgg
    21241 gcgacaaaaa aagttctagg attaatccta aatttactta atgatacaaa ttagagtgaa
    21301 tttttagccc ggaaagttgt ctcgttgtgt gagaggatgc gcttaccgga cgcataataa
    21361 accccatagc gttaccttca tttgccgcat caacaagttc agcatgctct tcttcagtca
    21421 aatcatctgt caaccaaccg atcaccacac tgtaattgcc cgtgcgtaaa gcgcgaacca
    21481 ttgactccac tgtgtggcaa ggggagagct ggctaatctg cattactttc gttaagggta
    21541 gcccagatgc ctgaacccat tcccgactca gtttttgttg cggtgttaac cagagttgcc
    21601 agcgcgattg ctgaccgagt tgctgtaaca atggcaacag tagaagttgc gtcatcatgg
    21661 gctgatcttc gcgatagaca acttcactga taagcccggc tgtagtgttt tccgtagaga
    21721 cacgcgcaat tttacttgct gcggatgaga acgacgaaga acgatgtgca tagcctgaag
    21781 tgtacataat caatccagcc cctgtgagtt actgtatgga tgtgcagtac atccagtgac
    21841 aacaaagatc aaccctattt tcggaaagag cctcgcaaat tttgtcgttg gtgacgggaa
    21901 aacataaatt aatcttgccc cttaagaata agttgcctat tttcgtagtt aacggatccg
    21961 ttaatgtgaa tcattctttt atgttatgat tttaaaagga attttatgaa aagcctctcc
    22021 tataagcgga tctataaatc acaagaatac ctggcaacgt tgggcacaat tgaataccga
    22081 tcattgtttg gcagttacag cctgaccgtt gacgacacgg tgtttgcgat ggtttctgat
    22141 ggtgagttgt atcttcgggc ttgtgagcaa agtgcacagt actgtgtaaa acatccgcct
    22201 gtctggctga catataaaaa gtgtggccga tccgttaccc tcaattacta tcgggttgat
    22261 gaaagtctat ggcgaaatca actgaagctg gtgcgtctgt cgaaatattc tctcgatgca
    22321 gcgctgaaag agaaaagcac gcgcaatacc cgggaaagac tgaaagattt gcccaatatg
    22381 tcttttcatc tggaagcgat tctcggggag gtg
//
LOCUS       DAJTRC010000061        22281 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.061, whole genome shotgun sequence.
ACCESSION   DAJTRC010000061 DAJTRC010000000
VERSION     DAJTRC010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 22281)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22281)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22281
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.061"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..1028)
                     /locus_tag="OO357_002552"
     CDS             complement(<1..1028)
                     /locus_tag="OO357_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5887460.1"
                     /translation="MLTKKKWALFSLLTLCGGTIYKLPSLKDAFYIPMQEYFHLTNGQ
                     IGNAMSVNSFVTTVGFFLSIYFADKLPRRYTMSFSLIATGLLGVYLTTMPGYWGILFV
                     WALFGVTCDMMNWPVLLKSVSRLGNSEQQGRLFGFFETGRGIVDTVVAFSALAVFTWF
                     GSGLLGFKAGIWFYSLIVIAVGIIIFFVLNDKEEAPSVEVKKEDGASQNTSMTSVLRD
                     KTIWLIAFNVFFVYAVYCGLTFFIPFLKNIYLLPVALVGAYGIINQYCLKMIGGPIGG
                     MISDKILKSPSKYLCYTFIISTAALVLLIMLPHESMPVYLGMACTLGFGAIVFTQRAV
                     FFAPIGEA"
     gene            complement(1130..2110)
                     /locus_tag="OO357_002553"
     CDS             complement(1130..2110)
                     /locus_tag="OO357_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HCR5887461.1"
                     /translation="MVTINNARKILQRVDTLPLYLHAYAFHLNMRLERVLPADLLDIA
                     SENNLRGVKIHVLDGERFSLGNMDDKELSAFGDKARRLNLDIHIETSASDKASIDEAV
                     AIALKTGATSVRFYPCYEGNLRDVLSIIANDIAYVRETYQDSGLTFTIEQHEDLKSHE
                     LVSLVKESEMESLSLLFDFANMINANEHPIDALKTMAPHITQVHIKDALIVKEQGGLG
                     HKACISGQGDMPFKALLTHLICLGDDEPQVTAYGLEEEVDYYASAFRFEDEDDNPWIP
                     YRQMSETPLPENHLLDARLRKEKEDAINQINHVRNVLQQIKQEANHLLNH"
     gene            complement(2118..2828)
                     /locus_tag="OO357_002554"
     CDS             complement(2118..2828)
                     /locus_tag="OO357_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
                     /protein_id="HCR5887462.1"
                     /translation="MAENQSTVENAKEKLDRWLKDGITTPGGKLPSERELGELLGIKR
                     MTLRQALLNLEAESKIFRKDRKGWFVTQPRFNYSPELSASFQRAAIEQGREPSWGFTE
                     KNRTSDIPETLAPLIAVTPSTELYRITGWGALEGHKVFYHETYINPEVAPGFIEQLEN
                     HSFSAVWEKCYQKETVVKKLIFKPVRMPGDISKYLGGSAGMPAILIEKHRADQQGNIV
                     QIDIEYWRFEAVDLIINL"
     gene            complement(3045..4868)
                     /gene="typA"
                     /locus_tag="OO357_002555"
     CDS             complement(3045..4868)
                     /gene="typA"
                     /locus_tag="OO357_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-dependent GTPase TypA"
                     /protein_id="HCR5887463.1"
                     /translation="MIEKLRNIAIIAHVDHGKTTLVDKLLQQSGTFDSRAETQERVMD
                     SNDLEKERGITILAKNTAIKWNDYRINIVDTPGHADFGGEVERVMSMVDSVLLVVDAF
                     DGPMPQTRFVTKKAFAYGLKPIVVINKVDRPGARPDWVVDQVFDLFVNLDATDEQLDF
                     PIVYASALNGIAGLDHEDMAEDMTPLYQAIVDHVPAPDVDLDGPFQMQISQLDYNSYV
                     GVIGIGRIKRGKVKPNQQVTIIDSEGKTRNAKVGKVLGHLGLERIETDLAEAGDIVAI
                     TGLGELNISDTVCDTQNVEALPALSVDEPTVSMFFCVNTSPFCGKEGKFVTSRQILDR
                     LNKELVHNVALRVEETEDADAFRVSGRGELHLSVLIENMRREGFELAVSRPKVIFREI
                     DGRKQEPYENVTLDVEEQHQGSVMQALGERKGDLKNMNPDGKGRVRLDYVIPSRGLIG
                     FRSEFMTMTSGTGLLYSTFSHYDDVRPGEVGQRQNGVLISNGQGKAVAFALFGLQDRG
                     KLFLGHGAEVYEGQIIGIHSRSNDLTVNCLTGKKLTNMRASGTDEAVVLVPPIRMTLE
                     QALEFIDDDELVEVTPTSIRIRKRHLTENDRRRANRAPKDD"
     gene            5241..6650
                     /gene="glnA"
                     /locus_tag="OO357_002556"
     CDS             5241..6650
                     /gene="glnA"
                     /locus_tag="OO357_002556"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--ammonia ligase"
                     /protein_id="HCR5887464.1"
                     /translation="MSAEHVLTMLNEHEVKFVDLRFTDTKGKEQHVTIPAHQVNAEFF
                     EEGKMFDGSSIGGWKGINESDMVLMPDASTAVIDPFFADSTLIIRCDILEPGTLQGYD
                     RDPRSIAKRAEDYLRSTGIADTVLFGPEPEFFLFDDIRFGSSISGSHVAIDDIEGAWN
                     SSTQYEGGNKGHRPAVKGGYFPVPPVDSAQDIRSEMCLVMEQMGLVVEAHHHEVATAG
                     QNEVATRFNTMTKKADEIQIYKYVVHNVAHRFGKTATFMPKPMFGDNGSGMHCHMSLS
                     KNGVNLFAGDKYAGLSEQALYYIGGVIKHAKAINALANPTTNSYKRLVPGYEAPVMLA
                     YSARNRSASIRIPVVSSPKARRIEVRFPDPAANPYLCFAALLMAGLDGIKNKIHPGEA
                     MDKNLYDLPPEEAKEIPQVAGSLEEALNELDLDREFLKAGGVFTDEAIDAYIALRREE
                     DDRVRMTPHPVEFELYYSV"
     gene            6823..7872
                     /gene="glnL"
                     /locus_tag="OO357_002557"
     CDS             6823..7872
                     /gene="glnL"
                     /locus_tag="OO357_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(II)"
                     /protein_id="HCR5887465.1"
                     /translation="MATGTQPDAGQILNSLINSILLIDDNLAIHYANPAAQQLLAQSS
                     RKLFGTPLPELLSYFSLNIELMQESLEAGQGFTDNEVTLVIDGRSHILSVTAQRMPDG
                     MILLEMAPMDNQRRLSQEQLQHAQQVAARDLVRGLAHEIKNPLGGLRGAAQLLSKALP
                     DPSLLEYTKVIIEQADRLRNLVDRLLGPQLPGTRVTESIHKVAERVVTLVSMELPDNV
                     RLIRDYDPSLPELAHDPDQIEQVLLNIVRNALQALGPEGGEIILRTRTAFQLTLHGER
                     YRLAARIDVEDNGPGIPPHLQDTLFYPMVSGREGGTGLGLSIARNLIDQHSGKIEFTS
                     WPGHTEFSVYLPIRK"
     gene            7884..9293
                     /gene="glnG"
                     /locus_tag="OO357_002558"
     CDS             7884..9293
                     /gene="glnG"
                     /locus_tag="OO357_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012133942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HCR5887466.1"
                     /translation="MQRGIVWVVDDDSSIRWVLERALAGAGLTCTTFENGAEVLEALA
                     SKTPDVLLSDIRMPGMDGLALLKQIKQRHPMLPVIIMTAHSDLDAAVSAYQQGAFDYL
                     PKPFDIDEAVALVERAISHYQEQQQPRNIQLNGPTTDIIGEAQAMQDVFRIIGRLSRS
                     SISVLINGESGTGKELVAHALHRHSPRTKAPFIALNMAAIPKDLIESELFGHEKGAFT
                     GANTIRQGRFEQADGGTLFLDEIGDMPLDVQTRLLRVLADGQFYRVGGYAPVKVDVRI
                     IAATHQNLEQRVQEGKFREDLFHRLNVIRVHLPPLRERREDIPRLARHFLQVAARELG
                     VEAKLLHPETEAALTRLAWPGNVRQLENTCRWLTVMAAGQEVLIQDLPGELFESTVAE
                     STSQMQPDSWATLLAQWADRALRSGHQNLLSEAQPELERTLLTTALRHTQGHKQEAAR
                     LLGWGRNTLTRKLKELGME"
     gene            9405..9515
                     /locus_tag="OO357_002559"
     CDS             9405..9515
                     /locus_tag="OO357_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YshB family small membrane protein"
                     /protein_id="HCR5887467.1"
                     /translation="MLESIINLVSSGAVDSHTPQTAVAAVLCAAMIGLFS"
     gene            complement(9744..11117)
                     /gene="hemN"
                     /locus_tag="OO357_002560"
     CDS             complement(9744..11117)
                     /gene="hemN"
                     /locus_tag="OO357_002560"
                     /EC_number="1.3.98.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709665.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HCR5887468.1"
                     /translation="MSVQQIDWDLALIQKYNYSGPRYTSYPTALEFSEDFGEQAFLQA
                     VARYPERPLSLYVHIPFCHKLCYFCGCNKIVTRQQHKADQYLDALEQEIVHRAPLFAG
                     RHVSQLHWGGGTPTYLNKAQISRLMKLLRENFHFNTDAEISIEVDPREIELDVLDHLR
                     AEGFNRLSMGVQDFNKEVQRLVNREQDEEFIFALLNHAREIGFTSTNIDLIYGLPKQT
                     PESFAFTLKRVAELNPDRLSVFNYAHLPTIFAAQRKIKDADLPSPQQKLDILQETIAF
                     LTQSGYQFIGMDHFARPDDELAVAQREGVLHRNFQGYTTQGDTDLLGMGVSAISMIGD
                     CYAQNQKELKQYYQQVYEQGNALWRGIALTRDDCIRRDVIKSLICNFRLDYAPLEKQW
                     DLHFADYFAEDLKLLAPLAKDGLVDVDEKGIQVTAKGRLLIRNICMCFDTYLRQKARM
                     QQFSRVI"
     gene            complement(11306..11815)
                     /gene="yihI"
                     /locus_tag="OO357_002561"
     CDS             complement(11306..11815)
                     /gene="yihI"
                     /locus_tag="OO357_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Der GTPase-activating protein YihI"
                     /protein_id="HCR5887469.1"
                     /translation="MKPSSSNSRSKGHAKARRKTREELDQEARDRKRQKKRRGHAPGS
                     RAAGGNTTSGSKGQNAPKDPRIGSKTPIPLGVTEKVTKQHKPKSEKPMLSPQAELELL
                     ETDECLDALLERLEAGETLSAEEQSWVDAKLDRIDELMQKLGLSYDDDEEEEEDEKQE
                     DMMRLLRGN"
     gene            12397..13029
                     /locus_tag="OO357_002562"
     CDS             12397..13029
                     /locus_tag="OO357_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418301.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HCR5887470.1"
                     /translation="MTNLNYQQTHFVMSAPDIRHLPSDTGIEVAFAGRSNAGKSSALN
                     TLTNQKSLARTSKTPGRTQLINLFEVADGKRLVDLPGYGYAEVPEEMKRKWQRALGEY
                     LEKRQSLQGLVVLMDIRHPLKDLDQQMIEWAVDSNIAVLVLLTKADKLASGARKALLN
                     MVREAVLAFNGDVQVETFSSLKKQGVDKLRQKLDTWFSEMQPVEETQDGE"
     gene            complement(13411..16197)
                     /gene="polA"
                     /locus_tag="OO357_002563"
     CDS             complement(13411..16197)
                     /gene="polA"
                     /locus_tag="OO357_002563"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HCR5887471.1"
                     /translation="MVQIPQNPLILVDGSSYLYRAYHAFPPLTNSAGEPTGAMYGVLN
                     MLRSLIMQYKPTHAAVVFDAKGKTFRDELFEHYKSHRPPMPDDLRAQIEPLHAMVKAM
                     GLPLLAVSGVEADDVIGTLAREAEKAGRPVLISTGDKDMAQLVTPNITLINTMTNTIL
                     GPEEVVNKYGVPPELIIDFLALMGDSSDNIPGVPGVGEKTAQALLQGLGGLDTLYAEP
                     EKIAGLSFRGAKTMAAKLEQNKEVAYLSYQLATIKTDVELELTCEQLEVQQPAAEELL
                     GLFKKYEFKRWTADVEVGKWLQAKGAKTAAKPQETSVADEAPEVTATVISYDNYVTIL
                     DEETLKAWIAKLEKAPVFAFDTETDSLDNISANLVGLSFAIEPGVAAYIPVAHDYLDA
                     PDQISHERALELLKPLLEDEKALKVGQNLKYDRGILANYGIELRGIAFDTMLESYILN
                     SVAGRHDMDSLAERWLKHKTITFEEIAGKGKNQLTFNQIALEEAGRYAAEDADVTLQL
                     HLKMWPDLQKHKGPLNVFENIEMPLVPVLSRIERNGVKIDPKVLHNHSEELTLRLAEL
                     EKKAHEIAGEEFNLSSTKQLQTILFEKQGIKPLKKTPGGAPSTSEEVLEELALDYPLP
                     KVILEYRGLAKLKSTYTDKLPLMINPKTGRVHTSYHQAVTATGRLSSTDPNLQNIPVR
                     NEEGRRIRQAFIAPEDYVIVSADYSQIELRIMAHLSRDKGLLTAFAEGKDIHRATAAE
                     VFGLPLETVTSEQRRSAKAINFGLIYGMSAFGLARQLNIPRKEAQKYMDLYFERYPGV
                     LEYMERTRAQAKELGYVETLDGRRLYLPDIKSSNGARRAAAERAAINAPMQGTAADII
                     KRAMIAVDAWLQAEQPRVRMIMQVHDELVFEVHKDDVDAVAKQIHQLMENCTRLDVPL
                     LVEVGSGENWDQAH"
     gene            16561..17493
                     /locus_tag="OO357_002564"
     CDS             16561..17493
                     /locus_tag="OO357_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709661.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5887472.1"
                     /translation="MANLLNKFIMTRILAAITLLLSIVLTILVTIFCSVPIIIAGIVK
                     LLLPVPVIWRKVSRFCDFMMYCWCEGLAVLLHLNPHLQWEVHGLEGLSKKNWYLLICN
                     HRSWADIVVLCVLFRKHIPMNKYFLKQQLAWVPFLGLACWALDMPFMKRYSRAYLLRH
                     PERRGQDVETTRRSCEKFRLHPTTIVNFVEGSRFTQEKHQQTHSTFQNLLPPKAAGIA
                     MALNVLGKQFDKLLNVTLCYPDNNRQPFFDMLSGKLTRIVVHVDLQPIADELHGDYIN
                     DKSFKRHFQQWLNSLWQEKDRLLTSLMSSQRQNK"
     gene            complement(17534..18964)
                     /locus_tag="OO357_002565"
     CDS             complement(17534..18964)
                     /locus_tag="OO357_002565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdgA family protein"
                     /protein_id="HCR5887473.1"
                     /translation="MIRKSATGVIVALAVIWGGGTWYTGTQIQPGIEKFIKDFNDAKK
                     KGEHAYDMTLSYKNFDKGFFNSRFQMQMTFDNGAPDLNIKPGQKVVFDVDVEHGPLPI
                     TMLMHGNVIPALAAAKVNLVNNELTQPLFIAAKNKSPVEATLRFAFGGSFSTTLDVAP
                     AEYGKFSFGEGQFTFNGDGSSLSNLDIEGKVEDIVLQLSPMNKVTAKSFTIDSLARLE
                     EKKFPVGESESKFNQINIINHGEDVAQIDAFVAKTRLDRVKDKDYINVNLTYELDKLT
                     KGNQQLGSGEWSLIAESIDPSAVRQFIIQYNIAMQKQLAAHPELANDEVALQEVNAAL
                     FKEYLPLLQKSEPTIKQPVRWKNALGELNANLDISIADPAKSSSSTNKDIKSLNFDVK
                     LPLNVVTETAKQLNLSEGMDAEKAQKQADKQISGMMTLGQMFQLITIDNNTASLQLRY
                     TPGKVVFNGQEMSEEEFMSRAGRFVH"
     gene            complement(19119..19745)
                     /gene="dsbA"
                     /locus_tag="OO357_002566"
     CDS             complement(19119..19745)
                     /gene="dsbA"
                     /locus_tag="OO357_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbA"
                     /protein_id="HCR5887474.1"
                     /translation="MKKIWLALAGLVLAFSASAAQYEDGKQYTTLEKPVAGAPQVLEF
                     FSFFCPHCYQFEEVLHISDNVKKKLPEGVKMTKYHVNFMGGDLGKDLTQAWAVAMALG
                     VEDKVTVPLFEGVQKTQTIRSASDIRDVFINAGIKGEEYDAAWNSFVVKSLVAQQEKA
                     AADVQLRGVPAMFVNGKYQLNPQGMDTSNMDVFVQQYADTVKYLSEEK"
     gene            complement(19762..20748)
                     /gene="srkA"
                     /locus_tag="OO357_002567"
     CDS             complement(19762..20748)
                     /gene="srkA"
                     /locus_tag="OO357_002567"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response kinase SrkA"
                     /protein_id="HCR5887475.1"
                     /translation="MNNSAFTFQTLHPDTIMDALFKQGIRVDSGLTPLNSYENRVYQF
                     QDEERRRFVVKFYRPERWTADQILEEHQFALQLVNDEVPVAAPVAFNGQTLLNHQGFY
                     FAVFPSVGGRQFEADNIDQMEAVGRYLGRMHQTGRKQLFIHRPTIGLNEYLIEPRKLF
                     EDATLIPSGLKAAFLKATDELIAAVTAHWREDFTVLRLHGDCHAGNILWRDGPMFVDL
                     DDARNGPAIQDLWMLLNGDKAQQRMQLETIIEAYEEFSEFDTAEIGLIEPLRAMRLVY
                     YLAWLMRHWADPAFPKNFPWLTGEDYWLRQTATFIEQAKVLQEPPLQLTPMY"
     gene            complement(20825..21094)
                     /locus_tag="OO357_002568"
     CDS             complement(20825..21094)
                     /locus_tag="OO357_002568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015966571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihD family protein"
                     /protein_id="HCR5887476.1"
                     /translation="MKCKRLNEVIELLQPAWQKEPDFNLLQFLQKLAKESGFDGELAD
                     LTDDILIYHLKMRDSAKDAVIPGLQKDYEEDFKTALLRARGVIKE"
     gene            21164..21748
                     /gene="mobA"
                     /locus_tag="OO357_002569"
     CDS             21164..21748
                     /gene="mobA"
                     /locus_tag="OO357_002569"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HCR5887477.1"
                     /translation="MNLMTTITGVVLAGGKARRMGGVDKGLLDLNGKPLWQHVADALK
                     TQLSHVVVNANRHQEIYQAGGLKVIEDSLADYPGPLAGMLSVMQQEAGEWFLFCPCDT
                     PYIPHDLAARLNHQRKDAPVVWVHDGERDHPTIALVNRAIEPLLLEYLQAGERRVMAF
                     MRLAGGHAVDFSDHKDAFVNVNTPEELARWQEKR"
     gene            21730..22257
                     /gene="mobB"
                     /locus_tag="OO357_002570"
     CDS             21730..22257
                     /gene="mobB"
                     /locus_tag="OO357_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HCR5887478.1"
                     /translation="MAGKTMIPLLAFAAWSGTGKTTLLKKLIPALCARGIRPGLIKHT
                     HHDMDVDKPGKDSYELRKAGAVQTIVASQQRWALMTETPDEEELDLHFLASRMDTSKL
                     DLILVEGFKHEEIAKIVLFRDGAGHRPEELVIDRHVIAVASDVPLNLDVALLDINDVE
                     GLADFVVEWMQKQNG"
BASE COUNT         5515 a         5583 c         5333 g         5850 t
ORIGIN      
        1 tttgcttcgc cgataggtgc aaaaaatacg gctcgctgtg taaagactat cgcgccaaag
       61 cccagcgtac atgccatccc taaatagacc ggcatacttt cgtgcggcag cataatcaac
      121 agtacgagcg cagcggtact gatgataaag gtgtagcata gatatttact cggcgatttc
      181 aggatcttat ctgaaatcat gccaccaatc ggtccaccaa tcattttcag acagtattgg
      241 ttaatgatgc cgtaagcccc caccagcgca acgggcaata gatagatgtt tttcaggaat
      301 ggaatgaaga atgtcaggcc acagtaaacc gcgtaaacga agaagacgtt gaaagcgata
      361 agccagatgg ttttgtctct cagcactgag gtcatactgg tgttttgcga tgctccgtct
      421 tcttttttca cctcaacgga cggtgcctct tctttgtcat tcaggacaaa gaaaataata
      481 atgcctacgg caatcacaat aagggaatag aaccagatgc ctgctttaaa acctaataag
      541 ccactgccaa accaggtaaa tactgccaac gcagaaaatg ccaccacggt atcgacaatg
      601 ccacgccctg tttcgaagaa gccaaacaac cgaccttgtt gttcgctatt gcccaatcga
      661 cttaccgact tgagcaagac cggccagttc atcatgtcgc aagtaacgcc aaatagcgcc
      721 cagacaaaga ggatgcccca ataccccggc attgtcgtca aataaacacc cagtaatcct
      781 gtcgcaatga gtgagaatga catggtgtat ctgcgcggta gtttatcggc aaaataaata
      841 gacagaaaaa agcccactgt ggtgacaaat gagtttaccg acatagcatt accaatttga
      901 ccattggtca aatggaaata ttcctgcatc gggatataaa acgcatcttt cagcgacggt
      961 aatttataaa ttgtaccgcc acacagtgtt aatagactaa ataacgccca tttctttttc
     1021 gtgagcataa catcccctaa gcgattttta aaatcactgt tttaaaggca ataagatacc
     1081 gaccattcat atggaatggt tttattaact gcgtatgagt taaaacttat tagtggttca
     1141 gaagatggtt tgcctcttgt ttgatttgtt gtagtacgtt acgcacatga tttatctgat
     1201 taattgcatc ttctttttct ttacgtaacc gcgcatccag taaatgattt tctggtaggg
     1261 gtgtttcact catctggcga taagggatcc acggattatc atcttcgtct tcaaagcgga
     1321 acgccgacgc atagtaatca acctcttctt ccaggccata tgccgtcacc tgcggctcat
     1381 catcgccaag gcagataagg tgcgttaata acgctttgaa tggcatatca ccctgacctg
     1441 aaatacaggc tttatgaccc aggccaccct gttctttaac gatcaaggca tctttaatat
     1501 ggacctgggt aatatgcggt gccatcgttt ttaaggcgtc gatgggatgc tcatttgcat
     1561 tgatcatgtt agcaaaatca aacagtaagg aaagagactc catctcactt tctttgacca
     1621 gcgacaccag ctcatgactt tttaaatctt catgctgctc aatcgtaaaa gtcaggccgc
     1681 tgtcctgata cgtttcccgt acataggcaa tgtcgttagc gataatcgat aatacgtcgc
     1741 gcaaattacc ttcataacat ggataaaaac gtacggacgt tgccccagtt ttcaacgcga
     1801 tggcgacggc ttcgtcaata gatgccttat ctgaagcgct ggtttcaata tgaatatcaa
     1861 ggttcagacg gcgggcttta tccccaaagg cagagagttc tttatcgtcc atattaccaa
     1921 gagaaaaacg ctcgccatcc agaacatgga ttttgacgcc acgtagatta ttttcactgg
     1981 cgatatcaag taaatcagca ggcaacactc tttccagccg catatttaaa tgaaaggcat
     2041 aagcatgtaa ataaagagga agagtgtcga cacgttgtag aatctttctt gcgttattta
     2101 ttgtcaccat aaaacaccta cagattaatg atgaggtcta cggcctcaaa tcgccaatat
     2161 tcaatatcta tctggacaat attgccttgc tggtcggcgc gatgcttttc aattaagatc
     2221 gctggcatac ccgcagaacc gccaagatac ttgctgatat cgcccggcat tctgacgggt
     2281 ttgaaaatca atttttttac taccgtctct ttttggtagc acttttccca gactgcagaa
     2341 aatgagtggt tttcaagttg ttcaataaaa cccggagcaa cttcaggatt aatatatgtt
     2401 tcgtgataga aaactttatg tccttccagc gccccccagc cggtgatgcg atagagttca
     2461 gttgatggcg tcactgcaat cagtggcgcg agcgtctcgg gaatatcgct ggtacggttt
     2521 ttctcggtaa acccccaaga aggctctcgc ccttgctcaa ttgcggcccg ctgaaagctc
     2581 gccgacagct ccggactgta attaaatcgc ggctgggtca cgaaccaccc cttacgatcc
     2641 ttacggaaga ttttggattc tgcctcgagg ttcaacaacg cctggcgcag cgtcatacgt
     2701 ttaatgccca gcagttctcc cagctctctt tctgaaggga gttttccacc cggcgtggtg
     2761 atgccatctt tcaaccaccg atccagtttc tcttttgcat tttctacggt ggattgattc
     2821 tccgccattg tttaatcctt ttggtttaaa ccagatgaag catcttacgc ttacgccaaa
     2881 aaagaaagca attaacggtg tatgactttg cagataattg tgacaaagct ctctattgac
     2941 gtaatcacag acttataagg gagatactcg cattggtgtg cgctcacgac aggtaaaaaa
     3001 aaacctgcca tcgctggcag gttttttatg actaaaaaac gaaattaatc gtctttcggt
     3061 gcgcggttgg cgcggcgacg atcgttttcc gtcaggtgac gtttacgaat acggatagag
     3121 gtcggagtca cttctaccag ttcgtcatca tcgatgaact ccagagcttg ttccagagtc
     3181 atgcggatag gcggaaccag aacaacagct tcgtcagtac cggaagcacg catgttggtc
     3241 agtttcttac cggtcaggca gtttacagtc aggtcgttag agcggctatg aataccgata
     3301 atctgacctt cgtaaacttc tgcaccgtga ccgaggaaca gcttaccgcg atcctgcaga
     3361 ccgaacagcg cgaacgcgac agctttaccc tgaccgttag agatcagtac accgttctga
     3421 cgctgaccca cttcgcccgg acgtacgtcg tcgtagtggc tgaaggtgga gtacagcaga
     3481 ccagtaccgg aagtcatggt catgaactca gaacggaagc caatcagacc acggcttggg
     3541 atcacgtagt cgagacgtac gcggccttta ccgtctggat tcatgttttt caggtcgcct
     3601 ttacgttcgc ccagcgcctg catcacagaa ccctgatgct gttcttcaac gtccagcgtc
     3661 acgttttcat acggctcttg tttacgaccg tctatttcac ggaagattac tttcggacgg
     3721 gataccgcca gttcgaaacc ttcacgacgc atgttttcga taagaacaga gaggtgcagt
     3781 tcaccacgac cagaaacgcg gaacgcatcg gcgtcttcgg tttcttctac gcgcagcgca
     3841 acgttgtgta ccagttcttt gttcagacga tcgaggatct gacgagacgt tacgaactta
     3901 ccttctttac cgcagaacgg cgaggtgtta acgcagaaga acatagaaac ggtcggctca
     3961 tcaacggaga gtgccggcag cgcttcaacg ttttgcgtgt cgcaaacggt gtcagaaatg
     4021 ttcagttcgc ccaggcccgt gatcgccacg atatcgccag cttccgccag atcggtttcg
     4081 atacgttcca gaccgaggtg gcccagcact ttaccgactt tcgcgttacg ggttttgcct
     4141 tcgctatcga tgatagtaac ctgctggttc ggcttcactt taccacgctt gatgcggcca
     4201 atgccgataa cgccaacata gctgttgtaa tcgagctgag aaatctgcat ctggaacgga
     4261 ccgtcaaggt caacgtccgg cgcaggaacg tggtcaacaa tcgcctgata cagcggggtc
     4321 atgtcttccg ccatatcttc gtggtccaga cccgcgatac cgttcagcgc agaagcgtaa
     4381 acgatcggga agtccagctg ctcgtcggtc gcgtcgaggt taacgaacag atcgaatacc
     4441 tgatccacaa cccaatcagg acgcgcgcca gggcggtcaa ctttgttgat aacaacaatc
     4501 ggcttcaggc cgtaagcaaa cgcttttttg gttacgaagc gcgtttgcgg catcgggccg
     4561 tcaaatgcgt caaccaccag cagcactgag tctaccatgg acattacacg ttcaacttca
     4621 ccaccgaagt cggcgtgccc cggggtatca acgatgttga tacggtaatc attccatttg
     4681 atagcggtgt ttttcgcgag gatggtaatc ccacgctctt tctccaaatc gttggagtcc
     4741 atcacgcgct cttgggtttc ggcacgagag tcgaacgtac cggattgttg gagcagcttg
     4801 tctaccaggg tggttttacc atggtctacg tgcgcgatga tggcgatatt acgcaatttt
     4861 tcgatcacaa ctttgcctca ggcattagaa atagcgcgtt attgtacacg gattaatcgc
     4921 actacaaaac aggatcacaa acatcctccg caaacaagta ttgcagagtc cctttgtgat
     4981 cgctttcacg gagcataaaa agggttatcc aaaggtcatt gcaccaatat ggtgcttaat
     5041 gtttccattg aagcactata ttggtgcaac attcacatca tggtgcagcc cttttgcacg
     5101 atgttaagca tgataacgcc ttttaggggc aatttaaaag ttggcacaga tttcgcttta
     5161 tcttttttac ggcgacacgg ccaaaataat tgcagatttc gttaccacga cgacaatgac
     5221 caatccagga gagttaaagt atgtccgctg aacacgtact gacgatgctg aacgagcacg
     5281 aagtgaagtt tgttgatttg cgcttcaccg ataccaaagg taaagaacaa cacgtcacta
     5341 tccctgctca tcaggtgaat gctgaattct tcgaagaagg caaaatgttt gacggctcct
     5401 cgattggcgg ctggaaaggc attaacgaat ccgacatggt gctgatgcca gacgcatcca
     5461 ctgcagtgat tgacccgttc ttcgccgact ccaccctgat tatccgttgc gacatccttg
     5521 aacctggcac cctgcaaggc tatgaccgtg acccgcgctc tattgcgaag cgcgccgaag
     5581 attacctgcg ttccactggc attgccgaca ccgtactgtt cgggccagaa cctgaattct
     5641 tcctgttcga tgacatccgt ttcggatcat ctatctccgg ttcccacgtt gctatcgacg
     5701 atatcgaagg cgcatggaac tcctccaccc aatacgaagg tggtaacaaa ggtcaccgtc
     5761 cggcagtgaa aggcggttac ttcccggttc cgccggtaga ctctgctcag gatattcgtt
     5821 ctgaaatgtg tctggtgatg gaacagatgg gtctggtggt tgaagcccat caccacgaag
     5881 tagcgactgc tggtcagaac gaagtggcta cccgcttcaa taccatgacc aaaaaagctg
     5941 acgaaattca gatctacaaa tatgtcgtgc ataacgtagc gcaccgcttc ggtaaaaccg
     6001 cgacctttat gccaaaaccg atgttcggtg ataacggctc cggtatgcac tgccacatgt
     6061 ctctgtctaa aaacggcgtt aacctgttcg caggcgacaa atacgcaggt ctgtctgagc
     6121 aggcgctgta ctacattggc ggcgtaatca aacacgctaa agcgattaac gccctggcaa
     6181 acccgaccac caactcttat aagcgtctgg tcccgggcta cgaagcaccg gtaatgctgg
     6241 cttactctgc gcgtaaccgt tctgcgtcta tccgtattcc ggtggtttct tctccgaaag
     6301 cacgtcgtat cgaagtacgt ttcccagatc cggcagctaa cccgtacctg tgctttgctg
     6361 ccctgctgat ggccggtctt gatggtatca agaacaagat ccatccgggc gaagccatgg
     6421 acaaaaacct gtatgacctg ccgccagaag aagcgaaaga gatcccacag gttgcaggct
     6481 ctctggaaga agcactgaac gaactggatc tggaccgcga gttcctgaaa gccggtggcg
     6541 tgttcacgga tgaagcgatc gatgcgtaca tcgctctgcg tcgcgaagaa gatgaccgcg
     6601 tgcgtatgac tccgcatccg gtagagtttg agctgtacta cagcgtctaa tagttgaagt
     6661 tgtactaccc ggcgcacaac gccgggattt agttgccgtg gaaactttca gcccatctct
     6721 gcatgggctt ttttctccgt caattctctg atgcttcgcg ctttttatcc gtaaaaagct
     6781 ataatgcact aaaatggtgc aacctgttca ggagactgct ttatggcaac aggcacgcag
     6841 cccgatgctg ggcagatcct caactcgctg attaacagta ttttgttaat tgatgacaat
     6901 ctggcgatcc attacgccaa ccccgccgcg caacaactgc tcgcccaaag ctcccgcaaa
     6961 ttgtttggta cgccgttacc ggaactgttg agctacttct cattaaatat cgagctgatg
     7021 caagaaagtc tggaggcggg gcaaggcttt accgataacg aagtgacgct ggtcatcgat
     7081 ggtcgctcgc atatcctttc tgtgacggcc cagcgtatgc cggacggcat gatcctgctg
     7141 gagatggcac cgatggataa ccagcgccgc ttaagccagg aacaactaca acatgctcaa
     7201 caagtagctg cccgtgattt agtgcgcggc ctggcacatg agattaaaaa tccgcttggc
     7261 ggtttacgtg gcgcggcgca gttactcagc aaagcgttac ctgacccatc actactcgaa
     7321 tataccaaag tgattatcga acaggcggac cggctgcgaa atttggtcga ccgtctgttg
     7381 gggccgcagc tgcccggtac gcgcgttacc gaaagtattc acaaagtggc tgaacgcgtg
     7441 gtaacgctgg tgtcgatgga actgccggac aacgtgcggt tgattcgtga ttacgatccc
     7501 agcctgccgg aactggcgca cgacccggat caaattgaac aggtcttgct gaatattgtg
     7561 cgcaatgcgc tacaggcgct ggggccggaa ggcggtgaaa tcattctgcg tacccgcacc
     7621 gcgtttcaac tgaccttaca cggcgagcgc taccggctgg cggcgcggat tgatgtggaa
     7681 gataacgggc cgggcattcc gcctcatttg caggatacgc tgttttaccc gatggtcagc
     7741 ggccgcgaag gtggcaccgg gcttggctta tccatcgccc gtaatttgat tgatcagcat
     7801 tcaggcaaaa ttgaatttac cagttggcca ggtcataccg agttctcggt ttacctgcct
     7861 atcaggaaat aaaggtgacg tttatgcaac gagggatagt ctgggtagtc gatgacgata
     7921 gttccatccg ttgggtgctt gaacgtgcgc tcgctggagc gggtttaacc tgtacgacat
     7981 ttgagaacgg cgcggaagta ctggaggcgc tggcgagcaa aacgccggat gtgctgcttt
     8041 cagatatccg tatgccggga atggacgggc tggcgctgct caagcagatt aaacagcgcc
     8101 atccaatgct tccggtcatc attatgaccg cacattccga tctggatgct gccgtcagcg
     8161 cctatcaaca aggggcgttt gattatctgc ccaaaccgtt tgatatcgac gaagcagtgg
     8221 cgctggttga gcgcgctatc agtcattacc aggaacagca gcagccgcgt aatattcagc
     8281 ttaacggccc aacgaccgat atcatcggcg aagcgcaagc catgcaggac gtgttccgta
     8341 ttatcggtcg gctttcgcgt tcttctatta gcgtgctgat taacggcgaa tccggcaccg
     8401 gtaaagaact ggtcgctcat gccctgcatc gccacagtcc gcgcaccaaa gcgccgttta
     8461 tcgcgctgaa tatggcagct atcccaaaag atttgatcga atcagaactg tttggccacg
     8521 agaaaggcgc gtttactggc gcgaatacca ttcgtcaggg gcgttttgaa caggccgatg
     8581 gcggcacgtt atttctcgac gaaattggtg atatgccgct ggatgtgcag acgcgtttgc
     8641 tgcgcgtgct ggcagacggt cagttttatc gcgttggcgg ctatgcgccg gtgaaagtgg
     8701 atgtgcggat tatcgctgcc actcaccaga atcttgaaca gcgggtgcag gaaggtaagt
     8761 tccgtgagga tctgttccac cgcctgaacg ttatccgcgt tcatctgccg ccgctgcgcg
     8821 aacgtcggga agatattccc cgtctggcgc gccatttttt acaggttgcc gcgcgcgaac
     8881 tgggcgtaga agcgaagttg ctgcatccgg aaaccgaagc tgctctgacg cgtctggcgt
     8941 ggccaggcaa cgtgcgccag ctggaaaaca cctgccgctg gctaacggtg atggccgccg
     9001 ggcaggaagt gttgattcag gatttgcccg gcgaactgtt tgaatcaacg gttgcggaga
     9061 gtacttcgca aatgcaaccg gacagttggg cgacactttt agcgcagtgg gcagacagag
     9121 cgctgcgttc cggtcatcaa aatctgcttt ccgaagcgca gccagagctg gagcggacgt
     9181 tactgacgac cgcgttgcga catacgcagg ggcataaaca ggaagcggcg cggctactcg
     9241 gctggggccg caacactctg acgcgtaagt taaaagagct ggggatggag tgattcacgg
     9301 cttgtgtgta aagattgatt attgagcgca aattgctggt attttgcgct ttactgttcc
     9361 gataagttca gtatgatctt gcccggaaaa cggggagagt cattatgctg gaatcaataa
     9421 ttaatctggt atcgagtggc gcagttgaca gccacacacc gcaaactgcc gttgctgccg
     9481 tgctgtgtgc cgcgatgatt gggctgttta gctgaagctc gttgtttatg ccggatgcgg
     9541 agaaagtcgt aggcctgata agacgcgacc agcgtcgcat caggcatcat gctcagatgc
     9601 ctgatgcggc gtaaacgcct tatccggcct acaaaagcaa taatttcaac aggttttgtt
     9661 gcccagcgca tcaggcaatc ttgttctcag cacattgatg gcgctttcgt tttctacttt
     9721 gtaaacgaag cgccattcac tctttaaatc acccgtgaga actgctgcat ccgcgctttc
     9781 tggcgcagat aggtatcaaa gcacatgcaa atgttgcgga tcagcaagcg acctttcgcc
     9841 gtcacctgta ttcccttctc atccacatcc accagcccat cttttgctaa cggggcgagc
     9901 agcttgagat cttccgcaaa gtaatcagcg aagtgcaaat cccactgttt ctcaagaggg
     9961 gcgtaatcca gacggaagtt gcagatgagc gacttaatca catcgcggcg aatacagtca
    10021 tcacgcgtta gcgcaatacc acgccacagc gcattgcctt gttcatacac ttgctgatag
    10081 tactgcttca actctttctg gttctgcgcg tagcagtcgc caatcatgct gatggcggaa
    10141 acgcccatcc ccagcagatc ggtatcgccc tgagtggtgt agccctggaa gttacgatgc
    10201 agcacgcctt cacgctgggc caccgccagc tcgtcatcag gacgggcaaa gtgatccata
    10261 ccgataaact gatagcccga ttgcgtcagg aaggcgatgg tttcctgcag gatatcgagt
    10321 ttttgctgcg gactcggcag gtcagcatct ttgattttgc gctgagcagc aaaaatggtc
    10381 ggcagatgcg cgtagttaaa aacgctcaga cgatcggggt tcagctccgc cacacgtttc
    10441 agggtaaagg cgaaactctc cggcgtctgt ttcggcaggc cgtaaatcag gtcgatgttg
    10501 gtggaggtaa aaccaatctc acgcgcatgg ttaagcagtg caaagatgaa ctcttcatcc
    10561 tgctcgcggt taaccagacg ttgcacttct ttgttgaagt cctgcacgcc catgctcagg
    10621 cgattaaagc cttcggcgcg taaatgatcg agtacatcca gttcgatttc ccgcggatcg
    10681 acttcgatcg aaatctccgc atcggtattg aaatggaagt tttcgcgcag cagcttcatc
    10741 agacggctga tttgcgcttt attcagatac gtcggcgttc cgccgcccca gtgcaattgg
    10801 ctgacgtgac gcccggcgaa cagcggtgca cgatggacga tttcttgctc cagcgcgtcc
    10861 agatactgat cggccttgtg ctgctggcga gtaacaatct tattgcaacc gcagaagtaa
    10921 caaagcttat ggcagaacgg gatatgtacg tagagagata atggacgctc aggatagcgc
    10981 gccacggctt gtaaaaacgc ctgttcgccg aagtcttctg aaaactccag cgcggtcggg
    11041 tacgaggtgt atcgtggccc ggaatagtta tatttctgga tcagggccag atcccagtcg
    11101 atttgctgta cagacatgct cactccttcc gatggcgtct ctggcggcta cggcgtaccg
    11161 gcggatgccc gtttcgggtc atcagccggt tgcgcagcca cttttgtcgc cgcgacaacc
    11221 gctgtagttt aacgaataac cacaccagat aacatataac cggaagggtt atcagcagaa
    11281 cgggaaggcc cacggcggca atccgttagt tgccccgcag cagacgcatc atgtcttctt
    11341 gcttctcgtc ttcttcctct tcttcgtcat cgtcataaga gaggccgagt ttctgcatca
    11401 actcatcaat acgatccagt ttagcatcca cccaggattg ctcttcggcg ctcagggttt
    11461 cgcctgcttc cagacgttcc agcagcgcat ccagacactc atccgtttcc agtaattcca
    11521 actccgcctg cggtgaaagc ataggtttct cactcttcgg tttgtgctgt ttggtgactt
    11581 tttcagtcac tcccaatgga ataggggttt tactgccaat acgtggatct tttggtgcgt
    11641 tctggccttt gctgcctgac gtggtgtttc cgcccgctgc gcggctgccc ggcgcatgac
    11701 cacgacgttt tttctgacgc ttacggtcac gagcttcctg atccagctcc tcgcgtgttt
    11761 ttcgacgcgc ttttgcatga cctttgctgc gtgagtttga agatgatggt ttcataatgg
    11821 ttcgtctttt agccgtttta ttcagtatag atttgcggcg gaatctaaca gaaagcaagc
    11881 aaagaaaaaa ggcgacagat tactctgtcg ccttttttcc tgactcataa cccttaacgg
    11941 gtcttacaat ccttgcaggc cgccaacaca ccctgttttt ccattagccg gaacaatccg
    12001 tgttgattcc atttccgttt aattacgtct ccggacgttt gtcttcctga caatcctctg
    12061 tcttcgcctc ctggcgctcc tgaccctcat cctgagtcat tgttcctgtt agcgtcctcg
    12121 ctctatgggt gctactttac gcctttgctt taaacaaaca atcaaccgcc atcagtattt
    12181 gcacattttc agagttttat ataaaagcaa ttcactgatt tatatagctt atttgacatg
    12241 catcgcagaa ttttctctgc tatttcgcat aaagactatc ggcaatgtct cacaaagcgt
    12301 atggctttta cttacaacgc tcacgccgaa aaatccgcct tttattatgg ttcaggtata
    12361 atcgccacaa agctttgact gacggagacg accgctttga ctaatttgaa ttatcaacag
    12421 acgcattttg tgatgagtgc gcctgatatt cgccacctac cttccgatac cggaattgaa
    12481 gtggcttttg caggccgttc caacgcaggt aaatccagcg cgctgaacac gctgactaac
    12541 cagaaaagcc tggctcgtac ctcaaaaacc ccagggcgca cccagcttat caacctgttt
    12601 gaagtggctg acggcaagcg tctggttgac ttgcctgggt acggttatgc ggaagtcccg
    12661 gaagagatga agcgcaaatg gcagcgtgcg ctcggcgaat acctcgaaaa acgtcagagc
    12721 ctgcaaggtc tggtggtgct aatggatatt cgccatccgc tgaaagattt ggatcagcag
    12781 atgattgagt gggcggtaga cagcaatatc gccgttctgg tgctgctgac caaagcggac
    12841 aaactggcaa gcggcgcacg taaagcgcta ttgaatatgg tgcgtgaagc tgtactggcg
    12901 tttaacggtg atgtgcaggt tgaaacgttt tcttcgttga agaaacaagg cgtagacaag
    12961 ctgaggcaga aactggatac ctggtttagc gagatgcagc ctgtagaaga aacgcaggac
    13021 ggcgaataat tttcttgcct taatgcttgt gccggatgtg gcgtatccgg cccgtaaatt
    13081 caatagatta caattctacc gttggcctga taaacatagc gcatcaggca ttacggatct
    13141 tttctttcgc ccaataaaaa acgccccagt cattactgac tggggcggct aaaatattca
    13201 gccaaatccg attacgtgaa gtaaaaggtc tgaaagatag aacatcttac ctctgtaccc
    13261 tacgcgacta actttactct tttttgttca gttcagaaag cactttttgt aatttttttt
    13321 cagttgttgc ataggaattt ctaatagcca tcacaaaacg tgacagctta tgttgcttac
    13381 ttacgaaaaa agggcatgtt caggcgaatc ttagtgcgcc tgatcccagt tttcgccact
    13441 ccccacttcc accagcaacg gcacatccag acgggtacag ttttccatca gttgatgaat
    13501 ctgcttcgcg acggcatcaa catcatcttt atgaacttca aataccagtt catcgtgtac
    13561 ctgcatgatc atacgtacac gcggttgctc agcctgtaac cacgcatcaa cggcaatcat
    13621 cgcccgtttg ataatgtcgg cggcggttcc ctgcattggc gcgttaatgg ctgcacgttc
    13681 agccgctgca cgacgagcac cattgctgga tttgatatcc ggcagataca gacggcgtcc
    13741 gtccagcgtt tcaacgtagc ccagctcttt cgcctgagca cgggtgcgtt ccatatactc
    13801 cagcacgcca gggtagcgtt cgaagtaaag gtccatgtac ttctgcgctt ctttacgtgg
    13861 aatgttcaat tgccgcgcca gaccgaaagc actcatgcca taaatcagac caaagttgat
    13921 cgctttcgcg ctacggcgtt gctcgctggt gacggtttcc agtggcaaac caaacacttc
    13981 tgccgccgtt gcccggtgga tatcttttcc ttccgcgaat gcggtcagca agcctttatc
    14041 acgcgagaga tgcgccataa tgcgcagttc aatctgcgag tagtccgctg agacaatcac
    14101 ataatcctct ggcgcaataa acgcctggcg gatacgacga ccttcttcgt tacgcaccgg
    14161 aatgttttgc aggttaggat cggttgacga taaacgtccc gttgcagtta ctgcctggtg
    14221 ataagaggta tgcacacgcc cggttttcgg gttgatcatc agcggcagct tgtcggtgta
    14281 agtcgacttc agcttcgcca gaccacgata ctccagaatc acttttggca acggatagtc
    14341 cagcgccagt tcttccagta cctcttccga cgttgacggc gcgccacccg gcgttttctt
    14401 cagcggttta ataccctgtt tttcaaacag aatggtttgc aactgcttgg tggaagaaag
    14461 gttaaattct tcacctgcaa tttcatgcgc tttcttttcc agctcagcca gacgcaaggt
    14521 gagctcttca gaatgattgt gcagcacttt cggatcgatc ttcacaccgt tacgttcaat
    14581 gcgtgaaagc accggcacca gcggcatttc gatattctcg aagacgttca acggcccttt
    14641 gtgtttctgc agatccggcc acattttcag atgcaactgc aaggtgacat ctgcatcttc
    14701 ggcggcgtaa cgtccggcct cttcgagggc aatctggtta aaggtcagtt gatttttgcc
    14761 tttacccgca atctcttcaa aagtgatggt tttgtgcttc aaccaacgtt ccgcgaggct
    14821 gtccatatcg tgacgcccgg caacgctatt gagaatgtag gactccagca tggtatcaaa
    14881 cgcaatccca cgcagttcaa tgccgtaatt cgccagaata ccgcgatcgt atttcaggtt
    14941 ttgcccgacc ttcagcgcct tttcatcttc cagcagcggt tttagcaact cgagtgcacg
    15001 ctcgtgagag atttgatcgg gcgcatcaag ataatcatga gcaaccggaa tatatgccgc
    15061 tacgcctggc tcgatagcaa aagaaagccc gaccaggtta gcagagatgt tatcaaggct
    15121 gtcggtttcg gtatcaaatg caaataccgg cgctttttcc agcttcgcaa tccacgcttt
    15181 cagtgtttct tcatcaagga tagtgacgta gttgtcataa gaaatcaccg ttgccgtcac
    15241 ttctggcgct tcgtctgcaa cactggtttc ctgaggcttc gcggctgttt ttgccccttt
    15301 ggcctgtaac catttgccca cttcgacatc agcagtccag cgtttgaact catacttttt
    15361 gaacagcccc aacaactctt ccgctgccgg ttgctgcact tccagttgtt cacaggtcag
    15421 ctccagttca acatcggttt taatcgtcgc caactggtat gagagataag caacttcttt
    15481 gttttgctcg agcttcgctg ccattgtttt cgcgccacgg aagctcaacc cagcaatttt
    15541 ttctggctcg gcatacagcg tatccagccc gccaagacct tgcagcaatg cctgcgcggt
    15601 tttttcaccg acgcccggta cgccaggaat gttatcagag gagtcaccca tcagcgccag
    15661 gaagtcgatg atcaattctg gcggcacgcc gtacttattc accacctctt ccggtccgag
    15721 gatggtattc gtcatggtat tgataagcgt aatatttggc gtcaccagct gcgccatatc
    15781 tttatcgcca gtgctaatca gcaccggacg cccggctttt tcggcttcgc gcgccagagt
    15841 accgataacg tcgtccgctt ctacgccaga aaccgccagc agcggcagtc ccatcgcttt
    15901 aaccatcgcg tgcagaggtt cgatttgtgc tcgcagatcg tccggcattg gcgggcgatg
    15961 tgatttgtaa tgttcaaaca gttcatcacg aaaggttttt cccttggcgt caaagaccac
    16021 cgctgcatgc gtcggtttat attgcatgat cagactgcgc agcatgttga ggacaccata
    16081 catcgcaccg gtcggctcgc ctgcgctgtt agtcagcggg ggaaacgcgt gatatgcgcg
    16141 ataaagataa gatgaaccat ctacaaggat aagtggattt tgggggatct gaaccataat
    16201 gtccgtgcct gtttatcaga ttatgggtaa aggatgccac agacaggatg aaaacatgag
    16261 tttttcaccg cttttcgtga aaatcttttg tagatctgct ggatcgctcg caaatcgtca
    16321 tgtggataac tttgtgcata aattatttac atcgctttta atttattatc atgcttttta
    16381 cagcatgatg tttgtctttg tttttcatgc agttaaaaat aaatccatcc ctaataattg
    16441 agataatggg attttttcta atgtggatag atatgaatta tttttctcct taaggatcat
    16501 ccgttatttg ggtcattttc tgctaaaatc tgcgccttgc gcttctttag tgcactgtct
    16561 atggctaacc ttttgaataa attcattatg acgagaatac tcgctgcgat aacccttttg
    16621 ctgagtatcg tattgaccat tttggtcact attttttgtt ccgtcccgat catcattgcc
    16681 gggattgtaa aactattgct acctgtgcca gtcatttggc gaaaggtttc gcgtttttgt
    16741 gattttatga tgtattgctg gtgtgaaggt ctggcggtat tactgcatct gaacccacac
    16801 ctgcaatggg aagttcacgg gctggaaggg ttaagtaaga agaactggta tctgcttatt
    16861 tgtaaccacc gtagttgggc agatattgtc gtactgtgcg tgctgtttcg taagcacatt
    16921 ccgatgaata aatatttcct caagcaacag ctggcctggg tgccatttct tggcctggcg
    16981 tgctgggcgc tggatatgcc gtttatgaag cgttattccc gcgcttattt attacgtcat
    17041 ccggagcgac gtggtcaaga tgttgaaacc actcggcgtt cttgtgaaaa gtttcgcctg
    17101 catcccacca ccattgtcaa tttcgttgaa ggctcccgtt tcacgcaaga aaaacatcag
    17161 caaacccact ccacttttca aaacttgttg ccaccaaagg cggcaggcat tgcgatggcg
    17221 ctaaatgtac tgggtaaaca attcgataaa ctgcttaatg ttacgctgtg ttatccggac
    17281 aataaccgtc agccgttctt cgatatgtta agcggtaaac tgacgcggat tgtcgtacat
    17341 gtggatttac agcctattgc cgatgagtta catggcgact acatcaatga caaaagcttt
    17401 aagcgccatt ttcagcaatg gctgaactca ttgtggcagg aaaaagaccg gcttctgacg
    17461 tctctcatgt catcacagcg tcagaataag taagttttac tcgcctctga atgagcagag
    17521 gcgagtgagt attttaatga acaaaacgtc cggcacgaga cataaattct tcttcgctca
    17581 tctcctgtcc gttaaaaaca actttacccg gtgtataacg cagctgcagc gaggcggtat
    17641 tgttgtcaat cgtgattaac tgaaacatct gacctaatgt catcatcccg ctgatttgtt
    17701 tatcagcctg cttttgcgct ttttccgcat ccattccttc agataaatta agctgttttg
    17761 cggtttctgt gacgacatta agcggtaact tcacatcaaa attgagcgat ttgatatctt
    17821 tgtttgtgga tgatgaagat ttggctgggt cggcaatact gatatccaga ttggcattta
    17881 gttcgccgag tgcgttcttc catcttaccg gttgtttaat ggtcggctca cttttttgta
    17941 ataacggtaa atactctttg aacaatgcag cattcacttc ttgcagagca acttcatcgt
    18001 ttgctaactc agggtgtgca gcaagctgct tctgcatcgc aatgttatac tggatgataa
    18061 attggcgcac tgctgaggga tcaatagatt cagcaatcaa tgaccactca ccactaccga
    18121 gttgctgatt cccttttgtt aacttatcaa gttcgtaggt cagattgaca ttgatataat
    18181 ctttgtcttt aacgcgatcc agcctggttt ttgcaacgaa agcatcgatt tgggcaacgt
    18241 cttccccgtg attgataatg ttaatctgat taaatttcga ctcgctttca ccaaccggaa
    18301 atttcttttc ttctaatcgc gccagagaat caatggtaaa actttttgcc gttactttgt
    18361 tcattggtga taattgcaga acaatatctt cgactttgcc ttcaatatcc aggttagaca
    18421 atgaactacc atcaccatta aaagtaaact ggccctcacc aaaagaaaat tttccatact
    18481 ctgcaggggc aacatctaat gtcgtagaga atgagccacc aaacgcgaat cgcaatgtcg
    18541 cttccacggg cgatttattt ttcgcggcga taaatagcgg ttgtgtcagt tcattattca
    18601 ctaagttcac ttttgccgct gccagtgctg ggatgacatt accatgcatt aacattgtga
    18661 tgggcaacgg accgtgctca acatccacat caaatacaac tttctggcct ggcttgatat
    18721 tgagatcggg tgcaccgtta tcgaatgtca tttgcatttg aaaacgggaa ttaaaaaagc
    18781 ctttgtcaaa gtttttataa cttaacgtca tatcgtaggc atgttcacct ttctttttcg
    18841 catcgttaaa atctttaata aacttttcga taccaggctg aatttgcgta cctgtgtacc
    18901 atgtgccacc accccagatt acggctaacg caacaataac acctgtagct gacttacgta
    18961 tcatataaat attccctgat aaaacagcaa aactttgaat atccacttat gctgaaataa
    19021 tgtccagcgg caggatgcat tatcagaatg tatttatatt tattaaataa taaaaaaagc
    19081 ccgtgaatat tcacgggctt tatgtaattt acattgaatt atttttcctc ggacagatat
    19141 ttcaccgtat cagcatactg ctgaacaaaa acatccatat tgctggtatc cataccctgc
    19201 ggattcagct gatatttacc gttaacaaac atcgccggaa caccacgcaa ttgcacatca
    19261 gctgcggctt tttcctgctg agcgaccaga gatttcacca cgaagctgtt ccacgccgcg
    19321 tcgtactctt cacctttaat acctgcattg ataaatacat cgcggatatc agatgctgaa
    19381 cgaatggtct gggttttctg tacgccttca aacagcggaa cagtcacttt gtcttccacg
    19441 cccagcgcca tcgccacagc ccatgcctga gtcagatctt tgcccaggtc accacccatg
    19501 aagttgacgt ggtatttagt catcttcacg ccttccggca gttttttctt cacattatca
    19561 gaaatatgca gaacttcttc aaactgatag cagtgcgggc agaagaaaga gaaaaactcc
    19621 agcacttgcg gcgcgccagc taccggtttt tccagggtag tgtactgttt accatcttca
    19681 tactgcgccg ccgatgcgct aaacgctaaa actaaaccag ccagcgccag ccaaatcttt
    19741 ttcatgatct actctctccg attaatacat aggtgttaat tgcaaagggg gttcttgtag
    19801 aacttttgcc tgttctataa aagtcgccgt ctgtcgtagc cagtaatctt ccccggttaa
    19861 ccacgggaaa tttttcggga acgcgggatc agcccaatgc cgcattagcc aggcaagata
    19921 ataaaccaaa cgcatggcgc gtaaaggttc aatcagtccg atttcagcgg tgtcgaactc
    19981 gctaaattct tcataagctt caataatagt ttccagttgc atccgctgct gggctttatc
    20041 gccattgagc aacatccaca aatcctgaat agctggacca ttacgtgcat catccagatc
    20101 aacaaacatt ggaccatcgc gccagagaat attcccggcg tggcagtctc catgtagccg
    20161 cagaacggtg aaatcttccc gccagtgtgc tgtaacggcg gcgatcagct catctgtcgc
    20221 tttcagaaat gccgctttca acccggaagg tatcagtgta gcgtcctcaa acagcttgcg
    20281 aggctcaatg agatattcat tcagaccgat ggtcgggcga tgaataaaaa gctgtttgcg
    20341 ccccgtctgg tgcatacgcc ctaaataacg cccaaccgct tccatctgat cgatattatc
    20401 agcttcgaac tggcgaccac cgacgcttgg aaaaacagcg aaataaaatc cctgatgatt
    20461 caataaagtc tgaccgttaa aggccacagg tgctgcgacc ggaacttcat catttaccag
    20521 ctgcaacgca aattgatgtt cttcgaggat ttgatcggct gtccaacgtt cagggcgata
    20581 aaatttgacg acaaaacgtc gacgctcttc gtcctgaaat tgatagacac ggttttcata
    20641 gctgttaagc ggggtaagac cggaatccac ccggatccct tgcttaaaca gagcgtccat
    20701 gatggtatcc gggtgtagtg tctggaaagt aaaagcgctg ttgttcatcc catcatccgg
    20761 aaaacattac gaatgatgaa ggatatcatt tcacgatgat tttggtggcg ccgcttacaa
    20821 gcttttactc tttaattacg ccgcgagcgc gtaacagcgc cgttttgaag tcttcttcat
    20881 aatctttctg caaacccggg atcaccgcat ctttcgcgga atcacgcatt ttcaggtgat
    20941 agatcagaat gtcatccgtc aaatccgcca gttcgccgtc aaaacctgac tctttcgcca
    21001 gtttctgcaa aaattgcaac aagttaaagt ctggctcttt ttgccaggct ggctggagga
    21061 gttcaataac ttcattcaga cgtttacatt tcatagtaat gctccttact cttgagacag
    21121 acacgttagc agggtcaatc ccacaataaa agaggcgata tcggtgaatc taatgacgac
    21181 gataacaggc gttgtgctgg caggcggtaa agccagacga atgggcggcg tagataaagg
    21241 attgctggac ttaaatggca aaccgctgtg gcaacatgtc gctgacgcgc ttaagacgca
    21301 gctctctcac gtcgtggtta atgctaatcg tcatcaggaa atctatcagg caggcggtct
    21361 gaaagtgatt gaagattcac tggcggatta cccaggccct ctggcaggaa tgctttcagt
    21421 aatgcagcag gaagctggtg agtggttttt gttttgtccg tgcgatacgc cttacattcc
    21481 ccatgattta gccgcccggc ttaatcatca gcgcaaagat gcgcctgtcg tgtgggtcca
    21541 tgacggtgaa cgcgatcacc cgactattgc tctggtaaac cgcgctattg agcctttatt
    21601 actggaatat ctgcaagcag gagaacgccg ggtaatggca tttatgcgtc tggctggcgg
    21661 tcatgcggtt gatttcagcg atcataaaga tgcatttgtt aacgtgaata cgccagagga
    21721 gcttgcccga tggcaggaaa aacgatgata ccgttactcg cctttgccgc gtggagtggt
    21781 accggaaaaa ctacgctttt gaaaaaactg atcccggcat tatgcgccag agggatccgt
    21841 ccggggctga ttaagcatac gcaccatgat atggatgttg ataagccagg caaagatagc
    21901 tatgagctgc gcaaggctgg cgcggtacaa accatcgttg ccagccagca acgatgggcc
    21961 ttgatgacgg aaacaccaga cgaagaagag ctggatctac attttctcgc aagtcgaatg
    22021 gatacctcaa agctggattt gattctggtc gaagggttta agcatgaaga gatcgcaaag
    22081 attgtgctgt ttcgcgatgg agccggacat cgacctgaag aattagtgat agacaggcat
    22141 gttattgctg tagccagtga tgtgccgctt aatcttgatg tcgcattact ggatattaat
    22201 gatgttgagg ggctggctga ttttgtggtg gagtggatgc aaaagcagaa cggataagca
    22261 agagtaggct ggatcagacg c
//
LOCUS       DAJTRC010000062        21502 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.062, whole genome shotgun sequence.
ACCESSION   DAJTRC010000062 DAJTRC010000000
VERSION     DAJTRC010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21502)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21502)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21502
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.062"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(9..1007)
                     /gene="fbp"
                     /locus_tag="OO357_002571"
     CDS             complement(9..1007)
                     /gene="fbp"
                     /locus_tag="OO357_002571"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 fructose-bisphosphatase"
                     /protein_id="HCR5887479.1"
                     /translation="MKTLGEFIVEKQHEFSHATGELTALLSAIKLGAKIIHRDINKAG
                     LVDILGASGAENVQGEVQQKLDLFANEKLKAALKARDIVAGIASEEEDEIVVFEGCEH
                     AKYVVLMDPLDGSSNIDVNVSVGTIFSIYRRVTPVGTPVTEEDFLQPGNKQVAAGYVV
                     YGSSTMLVYTTGCGVHAFTYDPSLGVFCLCQERMRFPEKGKTYSINEGNYIKFPNGVK
                     KYIKFCQEEDKSTNRPYTSRYIGSLVADFHRNLLKGGIYLYPSTASHPDGKLRLLYEC
                     NPMAFLAEQAGGKASDGKERILDIIPETLHQRRSFFVGNDHMVEDVERFIREFPDA"
     gene            1183..2556
                     /gene="mpl"
                     /locus_tag="OO357_002572"
     CDS             1183..2556
                     /gene="mpl"
                     /locus_tag="OO357_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-
                     meso-diaminopimelate ligase"
                     /protein_id="HCR5887480.1"
                     /translation="MRIHILGICGTFMGGLAMLARQLGHEVTGSDANVYPPMSTLLEK
                     QGIELIQGYDASQLEPQPDLVIIGNAMTRGNPCVEAVLEKNIPYMSGPQWLHDFVLRD
                     RWVLAVAGTHGKTTTAGMATWILEQCGYKPGFVIGGVPGNFEVSARLGESDFFVIEAD
                     EYDCAFFDKRSKFVHYCPRTLILNNLEFDHADIFDDLKAIQKQFHHLVRIVPGQGRII
                     WPENDINLKQTMAMGCWSEQELVGEQGHWQAKKLTTDASEWEVLLDGEKVGEVKWSLV
                     GEHNMHNGLMAIAAARHVGVAPADAANALGSFINARRRLELRGEANGVTVYDDFAHHP
                     TAILATLAALRGKVGGTARIIAVLEPRSNTMKMGICKDDLAPSLGRADEVFLLQPAHI
                     PWQVAEVAEACVQPAHWSGDVDTLADMVVKTAQPGDHILVMSNGGFGGIHQKLLDGLA
                     KKAEAAQ"
     gene            complement(2639..3190)
                     /locus_tag="OO357_002573"
     CDS             complement(2639..3190)
                     /locus_tag="OO357_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein"
                     /protein_id="HCR5887481.1"
                     /translation="MTKQPEDWLDDVPGDDIEDEDDEIIWVSKSEIKRDAEELKRLGA
                     EIVDLGKNALDKIPLDADLRPAIELAQRIKMEGRRRQLQLIGKMLRQRDVEPIRQALD
                     KLKNRHNQQVVLFHKLENLRDRLIDQGDDAIAEVLNLWPDADRQQLRTLIRNAKKEKE
                     GNKPPKSARQIFQYLRELAENEG"
     gene            3284..4636
                     /gene="pmbA"
                     /locus_tag="OO357_002574"
     CDS             3284..4636
                     /gene="pmbA"
                     /locus_tag="OO357_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease PmbA"
                     /protein_id="HCR5887482.1"
                     /translation="MALAMKVISQVEAQRKILEEAVSTALELASGKSDGAEVAVSKTT
                     GISVSTRYGEVENVEFNSDGALGITVYHQNRKGSASSTDLSPQAIARTVQAALDIARY
                     TSPDPCAGVADKELLAFDAPNLDLFHPAEVSPDEAIELAARAEQAALQADKRITNTEG
                     GSFNSHYGVKVFGNSHGMLQGYCSTRHSLSSCVIAEENGDMERDYAYTIGRAMSDLQT
                     PEWVGADCARRTLSRLSPRKLSTMKAPVIFANEVATGLFGHLVGAIAGGSVYRKSTFL
                     LDSLGKQILPDWLTIEEHPHLLKGLASTPFDSEGVRTERRDIIKDGILTQWLLTSYSA
                     RKLGLKSTGHAGGIHNWRIAGQGLSFEQMLKEMGTGLVVTELMGQGVSAITGDYSRGA
                     AGFWVENGEIQYPVSEITIAGNLKDMWRNIVTVGNDIETRSNIQCGSVLLPEMKIAGQ
                     "
     gene            4819..5201
                     /gene="cybC"
                     /locus_tag="OO357_002575"
                     /pseudo
     CDS             4819..5201
                     /gene="cybC"
                     /locus_tag="OO357_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313240.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b562"
     gene            complement(5246..5710)
                     /gene="nrdG"
                     /locus_tag="OO357_002576"
     CDS             complement(5246..5710)
                     /gene="nrdG"
                     /locus_tag="OO357_002576"
                     /EC_number="1.97.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate
                     reductase-activating protein"
                     /protein_id="HCR5887483.1"
                     /translation="MNYHQYYPVDIVNGPGTRCTLFVSGCVHECPGCYNKSTWRVNSG
                     QPFTKAMEDKIIKDLNDTRIKRQGISLSGGDPLHPQNVPDILKLVKRIRAECPGKDIW
                     VWTGYKLDELNAAQMQVVDLINVLVDGKFVQDLKDPSLIWRGSSNQVVHHLR"
     gene            complement(5868..8006)
                     /gene="nrdD"
                     /locus_tag="OO357_002577"
     CDS             complement(5868..8006)
                     /gene="nrdD"
                     /locus_tag="OO357_002577"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HCR5887484.1"
                     /translation="MTPHVMKRDGCKVPFKSERIKEAILRAAKAAEVDDADYCATVAA
                     VVNEQMQGRNQVDINEIQTAVENQLMSGPYKQLARAYIEYRHDRDIEREKRGRLNQEI
                     RGLVEQTNASLLNENANKDSKVIPTQRDLLAGIVAKHYARQHLLPRDVVQAHERGDIH
                     YHDLDYSPFFPMFNCMLIDLKGMLTQGFKMGNAEIEPPKSISTATAVTAQIIAQVASH
                     IYGGTTINRIDEVLAPFVTASYNKHRKTAEEWNIPDAEGYANSRTIKECYDAFQSLEY
                     EVNTLHTANGQTPFVTFGFGLGTSWESRLIQESILRNRIAGLGKNRKTAVFPKLVFAI
                     RDGLNHKKGDPNYDIKQLALECASKRMYPDILNYDQVVNVTGSFKTPMGCRSFLGVWE
                     NENGEQIHDGRNNLGVISLNLPRIALEAKGDEATFWKLLDERLVLARKALMTRIARLE
                     GVKARVAPILYMEGACGVRLNADDDVSEIFKNGRASISLGYIGIHETINALFSGEHVY
                     DNEKLRAKGIAIVERLRQAVDQWKEETGYGFSLYSTPSENLCDRFCRLDTAEFGVVPG
                     VTDKGYYTNSFHLDVEKKVNPYDKIDFEAPYPPLANGGFICYGEYPNIQHNLKALEDV
                     WDYSYQHVPYYGTNTPIDECYECGFTGEFECTSKGFTCPKCGNHDASRVSVTRRVCGY
                     LGSPDARPFNAGKQEEVKRRVKHLGNGQIG"
     gene            complement(8400..10055)
                     /gene="treC"
                     /locus_tag="OO357_002578"
     CDS             complement(8400..10055)
                     /gene="treC"
                     /locus_tag="OO357_002578"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HCR5887485.1"
                     /translation="MTNLPHWWQNGVIYQIYPKSFQDTTGSGTGDLRGVIQRLDYLHK
                     LGVDAIWLTPFYVSPQVDNGYDVANYTAIDPTYGTLDDFDELVTQAKSRGIRIILDMV
                     FNHTSTQHAWFREALNKESPYRQFYIWRDGEPETPPNNWRSKFGGSAWRWHAESEQYY
                     LHLFAPEQADLNWENPAVRAELKKVCEFWADRGVDGLRLDVVNLISKDPRFPEDLDGD
                     GRRFYTDGPRAHEFLHEMNRDVFTPRGLMTVGEMSSTSLEHCQRYAALTGSELSMTFN
                     FHHLKVDYPGGEKWTLAKPDFVALKTLFRHWQQGMHNVAWNALFWCNHDQPRIVSRFG
                     DEGEYRVPAAKMLAMVLHGMQGTPYIYQGEEIGMTNPHFTRITDYRDVESLNMFAELR
                     NDGRDADELLAILASKSRDNSRTPMQWSNGDNAGFTAGEPWIGLGDNYQQINVEAALA
                     DDSSVFYTYQKLIALRKQEAILTWGNYQDLLPNSPVLWCYRREWKGQTLLVIANLSRE
                     IQPWQPGQMRGNWQLVMHNYEEASPQPCAMNLRPFEAVWWLQK"
     gene            complement(10105..11526)
                     /gene="treB"
                     /locus_tag="OO357_002579"
     CDS             complement(10105..11526)
                     /gene="treB"
                     /locus_tag="OO357_002579"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS trehalose transporter subunit IIBC"
                     /protein_id="HCR5887486.1"
                     /translation="MMSKINQTDIDRLIELVGGRGNIATVSHCITRLRFVLNQPANAR
                     PKEIEQLPMVKGCFTNAGQFQVVIGTNVGDYYQALIASTGQAQVDKEQVKKAARQNMK
                     WHEQLISHFAEIFFPLLPALISGGLILGFRNVIGDLPMSNGQTLAQMYPSLQTIYDFL
                     WLIGEAIFFYLPVGICWSAVKKMGGTPILGIVLGVTLVSPQLMNAYLLGQQLPEVWDF
                     GMFSIAKVGYQAQVIPALLAGLALGVIETRLKRIVPDYLYLVVVPVCSLILAVFLAHA
                     LIGPFGRMIGDGVAFAVRHLMTGSFAPIGAALFGFLYAPLVITGVHQTTLAIDLQMIQ
                     SMGGTPVWPLIALSNIAQGSAVIGIIISSRKHNEREISVPAAISAWLGVTEPAMYGIN
                     LKYRFPMLCAMIGSGLAGLLCGLNGVMANGIGVGGLPGILSIQPSYWQVFALAMAIAI
                     IIPIVLTSFIYQRKYRLGTLDIV"
     gene            complement(11645..12592)
                     /gene="treR"
                     /locus_tag="OO357_002580"
     CDS             complement(11645..12592)
                     /gene="treR"
                     /locus_tag="OO357_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator TreR"
                     /protein_id="HCR5887487.1"
                     /translation="MQNRLTIKDIARLSGVGKSTVSRVLNNESGVSQRTRERVEAVMN
                     QHGFSPSRSARAMRGQSDKVVAIIVTRLDSLSENLAVQTMLPAFYEQGYDPIMMENQF
                     SPQLVAEHLGVLKRRNIDGVVLFGFTGITEEMLAHWQSSLVLLARDAKGFASVCYDDE
                     GAIKILMQRLYDQGHRNISYLGVPHSDVTTGKRRHEAYLAFCKAHKLHPVAALPGLAM
                     KQGYENVAKVITPETTALLCATDTLALGASKYLQEQRIDTLQLASVGNTPLMKFLHPE
                     IVTVDPGYAEAGRQAACQLIAQVTGRSEPQQIIIPATLS"
     gene            12777..12830
                     /gene="mgtL"
                     /locus_tag="OO357_002581"
     CDS             12777..12830
                     /gene="mgtL"
                     /locus_tag="OO357_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_003933616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mgtA regulatory leader peptide MgtL"
                     /protein_id="HCR5887488.1"
                     /translation="MEPDPTPLPRRRLKLFR"
     gene            12971..15667
                     /gene="mgtA"
                     /locus_tag="OO357_002582"
     CDS             12971..15667
                     /gene="mgtA"
                     /locus_tag="OO357_002582"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HCR5887489.1"
                     /translation="MFKEIFTRFIRHLPSRLVHRDPLPGAQQTVNTAVPPSLSAHCLK
                     MAVMPEEELWKTFDTHPEGLNQAEVESAREQHGDNKLPAQQPSPWWVHLWVCYRNPFN
                     ILLTILGAISYATEDLFAAGVIALMVAISTLLNFIQEARSTKAADALKAMVSNTATVL
                     RVINDKGENGWLEIPIDQLVPGDIIKLAAGDMIPADLRILQARDLFVAQASLTGESLP
                     VEKAATTRQPEHSNPLECDTLCFMGTTVVSGTAQAMVIATGANTWFGQLAGRVSEQES
                     EPNAFQQGISRVSMLLIRFMLVMAPVVLLINGYTKGDWWEAALFALSVAVGLTPEMLP
                     MIVTSTLARGAVKLSKQKVIVKHLDAIQNFGAMDILCTDKTGTLTQDKIVLENHTDIS
                     GKTSERVLHSAWLNSHYQTGLKNLLDTAVLEGTDEESARSLASRWQKIDEIPFDFERR
                     RMSVVVAENTEHHQLVCKGALQEILNVCSQVRHNGEIVPLDDTMLRKIKRVTDTLNRQ
                     GLRVVAVATKYLPAREGDYQRADESDLILEGYIAFLDPPKETTAPALKALKASGITVK
                     ILTGDSELVAAKVCHEVGLDAGEVVIGSDIETLSDDELANLAQRTTLFARLTPMHKER
                     IVTLLKREGHVVGFMGDGINDAPALRAADIGISVDGAVDIAREAADIILLEKSLMVLE
                     EGVIEGRRTFANMLKYIKLTASSNFGNVFSVLVASAFLPFLPMLPLHLLIQNLLYDVS
                     QVAIPFDNVDDEQIQKPQRWNPADLGRFMVFFGPISSIFDILTFCLMWWVFHANTPET
                     QTLFQSGWFVVGLLSQTLIVHMIRTRRVPFIQSCASWPLMIMTVIVMIVGIALPFSPL
                     ASYLQLQALPLSYFPWLVAILAGYMTLTQLVKGFYSRRYGWQ"
     gene            complement(15873..16259)
                     /gene="ridA"
                     /locus_tag="OO357_002583"
     CDS             complement(15873..16259)
                     /gene="ridA"
                     /locus_tag="OO357_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418664.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-iminobutanoate/2-iminopropanoate deaminase"
                     /protein_id="HCR5887490.1"
                     /translation="MSKTIATENAPAAIGPYVQGVDLGNMIITSGQIPVNPKTGEVPA
                     DVAAQARQSLDNVKAIVEAAGLKVGDIVKTTVFVKDLNDFATVNATYEAFFTEHNATF
                     PARSCVEVARLPKDVKIEIEAIAVRR"
     gene            complement(16332..16793)
                     /gene="pyrI"
                     /locus_tag="OO357_002584"
     CDS             complement(16332..16793)
                     /gene="pyrI"
                     /locus_tag="OO357_002584"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000148566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase regulatory
                     subunit"
                     /protein_id="HCR5887491.1"
                     /translation="MTHDNKLQVEAIKRGTVIDHIPAQIGFKLLSLFKLTETDQRITI
                     GLNLPSGEMGRKDLIKIENTFLSEEQVDQLALYAPQATVNRIDNYEVVGKSRPSLPER
                     IDNVLVCPNSNCISHAEPVSSSFAVRKRANDIALKCKYCEKEFSHNVVLAN"
     gene            complement(16806..17741)
                     /gene="pyrB"
                     /locus_tag="OO357_002585"
     CDS             complement(16806..17741)
                     /gene="pyrB"
                     /locus_tag="OO357_002585"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase"
                     /protein_id="HCR5887492.1"
                     /translation="MANPLYQKHIISINDLSRDDLNLVLATAAKLKANPQPELLKHKV
                     IASCFFEASTRTRLSFETSMHRLGASVVGFSDSANTSLGKKGETLADTISVISTYVDA
                     IVMRHPQEGAARLATEFSGNVPVLNAGDGSNQHPTQTLLDLFTIQETQGRLDNLHVAM
                     VGDLKYGRTVHSLTQALAKFDGNRFYFIAPDALAMPQYILDMLDEKGIAWSLHSSIEE
                     VMAEVDILYMTRVQKERLDPSEYANVKAQFVLRASDLHNAKANMKVLHPLPRVDEIAT
                     DVDKTPHAWYFQQAGNGIFARQALLALVLNRDLVL"
     gene            complement(17745..17879)
                     /gene="pyrL"
                     /locus_tag="OO357_002586"
     CDS             complement(17745..17879)
                     /gene="pyrL"
                     /locus_tag="OO357_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyr operon leader peptide"
                     /protein_id="HCR5887493.1"
                     /translation="MVQCVRHFVLPRLKKDAGLPFFFPLITHSQPLNRGAFFCLGVRR
                     "
     gene            complement(18160..18555)
                     /locus_tag="OO357_002587"
     CDS             complement(18160..18555)
                     /locus_tag="OO357_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5887494.1"
                     /translation="MVERTAVFPAGRHSLYAEHRYSAAIRSGDLLFVSGQVGSREDGT
                     PEPDFQQQVRLAFDNLHATLAAAGCTFDDIIDVTSFHTDPEKQSEDIMTVKNEIFSAP
                     PYPNWTAVGVTWLAGFDFEIKVIARIPEL"
     gene            complement(18687..19400)
                     /gene="bdcA"
                     /locus_tag="OO357_002588"
     CDS             complement(18687..19400)
                     /gene="bdcA"
                     /locus_tag="OO357_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5887495.1"
                     /translation="MGAFTGKTVLILGGSRGIGAAIVRRFVTDGANVRFTYAGSKDAA
                     ERLAQETGATAVFTDSADRDAVIDVVRKSGALDILVVNAGIGVFGDALELNADDIDRL
                     FKINIHAPYHASVEAARQMPEGGRILIIGSVNGDRMPVAGMAAYAASKSALQGMARGL
                     ARDFGPRGITINVVQPGPIDTDVNPANGPMRDMLHSFMAIKRHGQPEEVAGMVAWLAG
                     PEASFVTGAMHTIDGAFGA"
     gene            19471..20064
                     /locus_tag="OO357_002589"
     CDS             19471..20064
                     /locus_tag="OO357_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709951.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HCR5887496.1"
                     /translation="MVTKKQSRVPGRPRRFAPEQAVSAAKVLFHQKGFDAVSVSEVTD
                     YLGINPPSLYAAFGSKAGLFSRVLNEYVGTEAIPLADILRDDRPVGECLVEVLKEAAR
                     RYSQNGGCAGCMVLEGIHSHDPQARDIAIQYYHAAETTIYDYIARRHPQSAQCVTDFM
                     STVMSGLSAKAREGHSIEQLCATAALAGEAIKTLLKE"
     gene            20209..20661
                     /gene="tabA"
                     /locus_tag="OO357_002590"
     CDS             20209..20661
                     /gene="tabA"
                     /locus_tag="OO357_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin biofilm protein TabA"
                     /protein_id="HCR5887497.1"
                     /translation="MIIGNIHNLQPWLPQELRQAIEHIKAHVTAETPKGKHDIEGNRL
                     FYLISEDMTEPYEARRAEYHARYLDIQIVLKGQEGMTFSTQPAGAPDTDWLADKDIAF
                     LPEGVDEKTVILNEGDFVVFYPGEVHKPLCAVGAPAQVRKAVVKMLMA"
     gene            20784..>21502
                     /locus_tag="OO357_002591"
     CDS             20784..>21502
                     /locus_tag="OO357_002591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HCR5887498.1"
                     /translation="MGKISDLNYSQHITLADNFKQKNEALDTWYVGMNDFARIAGGQN
                     SRSNILSPRAFLEFLAKIFTLGYVDFSKRSNEAGRNMMAHIEFSSYSKDTDGNEKMKF
                     YMNNPEGERADLSKVKIEITLASASTKGIREGHTVIIFKQSDGSTNRYEGKSFERKDD
                     SSLHLITNKVLACYQREANKKIARLLNNHQKLNNIQELNDSQELNNSQKLNNSQELNN
                     SQVSCKGSVDSTITDLLEKAL"
BASE COUNT         5194 a         5323 c         5662 g         5323 t
ORIGIN      
        1 gcaggagatt acgcgtccgg gaactcacgg ataaagcgtt cgacatcttc aaccatatgg
       61 tcgttgccga caaagaacga acggcgctgg tgcagagttt ccgggatgat atccagaata
      121 cgctctttgc catcgctcgc tttaccgccc gcttgttccg ccaggaatgc catcgggttg
      181 cactcataaa gcaaacgcag tttgccgtcc gggtggctgg cggtgcttgg gtagagataa
      241 ataccgcctt tcagcaggtt acggtggaaa tccgcaacca gtgaaccgat ataacgcgag
      301 gtatacgggc gattggtgga tttatcttct tcctggcaga atttaatgta cttcttcacc
      361 ccgttcggaa acttaatgta gtttccttcg ttgatggagt aggttttgcc tttctccggg
      421 aagcgcatcc gttcctggca caggcagaaa acgcccagcg aaggatcgta agtaaaggcg
      481 tgaacaccgc atccggtggt gtaaaccagc atggtagagg agccgtatac cacgtaacct
      541 gccgcaacct gtttgttacc aggctggagg aaatcttctt ccgttaccgg cgtgccaaca
      601 ggcgtaacgc ggcggtagat ggagaaaatg gtaccgacag agacgttaac atcgatgttg
      661 gacgagccat ccagggggtc catcagcacc acgtattttg cgtgttcaca gccttcaaag
      721 acgacaatct catcttcttc ttcagaggca atccccgcaa cgatatcgcg tgctttcagt
      781 gcggctttca gtttttcatt agcgaacaag tcgagttttt gctgaacctc gccctgcacg
      841 ttctcagcac cgctggcacc caggatatca accagtcctg ctttgttgat atcgcgatgg
      901 ataatcttgg cgcccagttt tattgccgac agcaaagcag tgagctcacc ggtagcatga
      961 gaaaactcgt gctgcttttc gacaataaat tcacctaacg ttttcataaa actttccctg
     1021 caatgtttat ggagtaaagc gaccgcaaca atcttaacaa ataatctcaa tgttgcgctc
     1081 aggtgaatcg cgccagcaaa ttgcggatta tcctgaaatg cgtttctcac ttgcccgaca
     1141 tatgcgtaaa atgagcggca gattaaaaaa ggatagtgac gtatgcgcat tcatatttta
     1201 ggaatttgtg gcacatttat gggcggtctg gcgatgctgg cgcgccagtt aggccatgaa
     1261 gtaacgggtt cggacgccaa tgtgtatccg ccgatgagca ccttacttga gaagcaaggc
     1321 attgagctga ttcagggtta cgatgccagc cagctcgagc cgcagccgga tctggtgatt
     1381 attggcaacg ccatgacccg tggaaatccg tgtgtggaag cggtactgga aaaaaacatc
     1441 ccttatatgt caggtccaca gtggctgcac gattttgtgc tgcgcgaccg ctgggtgctg
     1501 gccgttgccg gtacacacgg caaaaccacc accgcgggaa tggcgacctg gattctggaa
     1561 cagtgtggtt acaaaccggg atttgtgatc ggcggtgtgc cggggaactt tgaggtttcg
     1621 gcgcgtctgg gcgaaagcga cttctttgtt atcgaagcgg atgagtacga ctgcgccttc
     1681 ttcgacaaac gctctaaatt tgtccattac tgcccgcgta cgctgatcct caacaacctt
     1741 gagttcgatc acgccgatat ctttgacgac ctgaaagcga tccagaaaca gttccaccat
     1801 ctggtgcgta tcgttccggg gcagggccgt attatctggc cggaaaatga catcaacctg
     1861 aaacagacca tggcgatggg ctgctggagc gagcaggagc tggtgggtga gcagggtcac
     1921 tggcaggcga aaaagctgac caccgatgct tccgaatggg aagttttgct ggatggcgaa
     1981 aaagtgggcg aagtgaaatg gtcgctggta ggcgaacata atatgcacaa tggcctgatg
     2041 gcgattgcag cggctcgcca tgttggtgta gcgccggcag atgccgctaa cgcgctgggt
     2101 tcgtttatta atgctcgtcg ccgtctggag ttgcgtggtg aagcgaatgg cgtcacggta
     2161 tatgacgatt ttgcccatca cccgacggcg attctggcaa cgctggcggc gctgcgtggc
     2221 aaagttggtg gtacggcgcg cattattgct gtgctggaac cgcgctcgaa taccatgaaa
     2281 atggggatct gcaaagacga tctggcacct tcattaggtc gtgctgatga agtcttcctg
     2341 ctgcaaccgg cgcatattcc gtggcaggtg gcagaagtgg cagaagcctg cgttcagcct
     2401 gcacactgga gtggcgatgt ggatacgctg gcagatatgg tggtgaaaac cgctcagcct
     2461 ggcgaccata ttctggtgat gagcaacggc ggttttggtg ggatccatca gaaactgctg
     2521 gatggtctgg cgaagaaggc ggaagctgcg cagtaattcg gcttcagcct gatatagcat
     2581 tgccggataa ggcgtttacg ccgcatccgg caaaccatac tcacatcaac aacgaaaatt
     2641 acccttcatt ctccgccagt tcgcgcagat actggaaaat ctggcgtgcg gatttcggcg
     2701 gcttattccc ttctttctct ttcttcgcgt tgcggatcag tgtacgcagt tgctgacgat
     2761 cggcatcggg ccacagattc aatacttcag cgatggcgtc atcaccttga tcgatcagac
     2821 gatcgcgcag gttttccagt ttatggaaca gcaccacctg ctggttgtga cggtttttca
     2881 gcttatccag cgcctgacga ataggctcta cgtcgcgctg gcgcagcatc ttaccgatga
     2941 gctgcaactg gcggcggcga ccttccatct taatacgctg ggcgagctca atagccgggc
     3001 gcagatccgc atctaacggg atcttatcca gcgcgttttt ccccagatca acaatttccg
     3061 cgccaaggcg ttttagctcc tcggcatcac gtttaatttc acttttactg acccagataa
     3121 tttcatcgtc ttcgtcttcg atgtcatcac cgggaacgtc gtcgagccag tcttcgggct
     3181 gcttagtcat ctcaggctcc ttaaaaaaag aggctaatgt taccagttaa gatgcgcact
     3241 gaaaaacggt tctctgttag acttcagaga aactctctac attatggcac ttgcaatgaa
     3301 agtaatctct caagttgaag cgcagcgcaa gattctggaa gaagcagttt ccactgcgct
     3361 ggagttggcc tcaggcaaat cggacggtgc ggaagttgcc gtcagcaaga ccaccggcat
     3421 tagcgtaagc acgcgttatg gtgaagtgga gaatgttgaa ttcaatagtg atggcgcgct
     3481 ggggatcact gtttatcacc agaaccgtaa aggtagcgca tcgtctaccg atttaagccc
     3541 gcaggccatt gcccgcaccg tacaggcggc gctggatatt gcccgttata cctcgccaga
     3601 tccctgtgcc ggcgtggcag acaaagagct gctggccttt gacgcaccga atctcgactt
     3661 gttccaccct gcggaagttt ccccggatga agccattgaa ctggcggccc gcgcagaaca
     3721 ggcggcattg caggcggaca aacgcatcac caataccgaa ggtggcagct ttaacagcca
     3781 ctacggtgtc aaagtttttg gcaacagcca cggcatgttg cagggttact gctcaacgcg
     3841 tcattcgctc tccagctgtg taattgccga agaaaatggc gatatggagc gtgattacgc
     3901 ctacaccatt ggccgcgcga tgagcgatct gcaaacgcca gagtgggtcg gggccgactg
     3961 tgctcgccgt actttatcgc gcctgtcacc gcgtaaactc tccaccatga aagcgccggt
     4021 catttttgcc aatgaagtgg caaccgggct ttttggccat ctggtggggg cgatagcggg
     4081 tggctcggtt taccgtaaat ctacctttct gctggattcg ctgggtaaac aaattctgcc
     4141 ggactggctg accattgaag agcatccgca tctgctgaaa gggctggcgt cgacgccatt
     4201 cgacagcgaa ggtgtgcgca ccgagcgtcg cgatattatt aaagatggca tcctgactca
     4261 gtggctgctg accagctact cggcgcggaa actggggctg aaaagcaccg gacatgcggg
     4321 cggtattcac aactggcgga ttgccggaca agggctaagc ttcgaacaaa tgctcaaaga
     4381 gatgggcacc gggctggtgg tgacggagct gatgggccag ggcgtgagtg ccattaccgg
     4441 tgattattcc cgtggtgcag cgggcttctg ggtagagaat ggcgaaattc agtatcccgt
     4501 tagtgaaatc accatcgcag gtaatttaaa agatatgtgg cgcaatattg tcaccgtcgg
     4561 taacgatatt gaaacacgca gtaatataca gtgtggttct gtgctgttgc cggagatgaa
     4621 aatcgccggg cagtaaaaaa ctggcgcgac ctgccgcgct ataaactaaa aaattacaca
     4681 aaatcattcg cactgcatcg agacggaaag tgaatgaggg ccggggagcg tactcgcagt
     4741 acgtgacccg ggcgaatgag cgtagccgtc gatgaggcag tgtgaaggat gaagtgtaaa
     4801 taaaaaagga agtgagcaat gcgtaaaagc ctgttagcta ttcttgcagt ctcctcgttg
     4861 gtattcagtt ctgcgtcgtt tgccgctgat cttgaagaca atatggaaac cctcaacgac
     4921 aatttaaaag tgatcgaaaa agcggataac gcggcgcaag tcaaagacgc gttaacgaag
     4981 atgcgcgccg cagcgctgga tgcgcaaaaa gcaacgccgc cgaagctcga agataaatca
     5041 ccggacagcc cggaaatgaa agatttccgc cacggtttcg acattctggt cggtcagatt
     5101 gacgacgcgc tgaagctggc aaatgaaggt aaaaagaagc gcaggctgct gcagagcaac
     5161 tgaaaacgac ccgcaacgcc tatcaccaga agtatcgtta attcctcatt tccctgttgc
     5221 ctgcactcag gtaacaggga acttatcatc gcaaatgatg caccacctga ttgctgctgc
     5281 cgcgccagat cagggaaggg tcttttaaat cctgcacaaa tttgccgtcg accagcacgt
     5341 taatcaaatc aacaacctgc atttgcgcag cgttgagttc gtcgagttta tagcctgtcc
     5401 acacccagat gtctttaccc ggacactcgg cgcggatgcg ttttaccagt ttcagaatat
     5461 ccggcacgtt ttgcggatgc agcggatcgc cgccggagag ggaaatcccc tggcgtttga
     5521 tacgagtgtc attcagatct ttaatgatct tgtcttccat tgctttggta aatggctgac
     5581 cagaatttac ccgccatgtg cttttgttat agcaaccggg gcattcatga acacacccgg
     5641 agacaaacag ggtgcagcga gtgccggggc cgttgacgat gtcgacagga tagtactgat
     5701 gataattcat tatttcgcct ggcttatcag tccgcgtgta ggctgcatcc ggtaatcaat
     5761 gcctgatgcg acgctgtcgc gtcttatcag gcctacagcc gttgccaaat gtaggccgga
     5821 taaggcgttt acgccgcatc cggcatttgc ttagcgcaga gtgaagatta acctatctgc
     5881 ccattcccca aatgtttaac gcggcgctta acttcttcct gcttaccagc gttaaacgga
     5941 cgtgcatccg ggctacctaa atatccgcac acgcggcgag ttaccgacac acgggaggcg
     6001 tcatggttac cacatttcgg gcaagtgaag cctttgctgg tgcactcgaa ctcaccggta
     6061 aagccacact cgtagcactc atcaatcggt gtattggtgc cgtaatacgg tacatgctga
     6121 tagctgtaat cccagacatc ttccagcgct ttcaggttgt gctgaatgtt tggatactcg
     6181 ccgtagcaaa tgaaaccacc gttcgccagc ggcgggtaag gcgcttcaaa gtcgatcttg
     6241 tcgtacgggt tcaccttctt ctccacatca aggtggaaac tgttggtgta gtaaccttta
     6301 tcggtcacgc ccggcaccac gccaaactca gcagtatcga gacggcagaa gcggtcgcac
     6361 aggttttcac tcggcgtgct gtaaagactg aaaccataac ccgtttcttc tttccactga
     6421 tccactgcct gacgcagacg ttcaacaatg gcgatacctt tcgcgcgaag cttctcgttg
     6481 tcgtaaacat gctcgccgct gaacagcgcg ttaatggttt cgtggatgcc gatgtaaccc
     6541 agcgaaatag acgcacgacc gtttttgaag atttcagaaa catcatcgtc agcattcaga
     6601 cgtacgccac aagcaccttc catatagagg atcggggcga cgcgcgcttt cacgccttcg
     6661 agacgagcga tacgggtcat cagtgcctta cgtgccagca ccagacgttc atccagcagc
     6721 ttccagaagg tggcttcatc gccttttgct tccagagcaa tacgcggcag gttcaggctg
     6781 atcacgccga ggttgttacg accatcgtgg atctgctcgc cgttttcatt ttcccacacg
     6841 ccgaggaagc tgcggcagcc catcggggtt ttaaacgaac cggtgacgtt cactacctga
     6901 tcgtagttca ggatatccgg atacatgcgc ttgcttgcgc actccagcgc cagctgtttg
     6961 atgtcgtagt tcggatcgcc ttttttatgg ttcaggccat cgcgaatcgc aaacaccagt
     7021 ttcgggaaca ccgcagtttt acggttttta ccgaggcctg cgatacggtt acgcaggatc
     7081 gattcctgaa tcaggcgcga ttcccagctg gtgcccagac caaaaccaaa ggttacaaac
     7141 ggcgtctgac cgttggcggt gtgcagggtg tttacttcgt actccagcga ctggaaggca
     7201 tcgtagcact ctttgatggt acgagagtta gcgtagcctt cggcgtccgg gatgttccac
     7261 tcttctgcgg tcttgcgatg tttgttgtag ctggcggtaa caaacggcgc cagcacttca
     7321 tcgatacggt taatggtggt gccgccataa atatggctgg cgacctgagc aataatctgc
     7381 gcagttaccg cggttgccgt agagatcgac ttcggcggtt caatctcggc gttccccatt
     7441 ttaaaaccct gggtcagcat gcctttcagg tcgatcaaca tgcagttgaa catcgggaag
     7501 aacggcgagt aatcgagatc gtgatagtga atatcgccac gctcatgtgc ctgcaccacg
     7561 tcacgcggca gcaggtgctg gcgtgcatag tgtttagcca cgatcccggc cagcaggtcg
     7621 cgctgggttg gaatcacctt gctgtctttg ttggcgtttt cgttgagtaa cgaggcgttg
     7681 gtctgctcga ccagaccacg gatctcctgg ttcaggcgac cgcgtttttc acgttcaatg
     7741 tcgcgatcgt gacggtactc gatgtaagca cgagccagtt gtttgtatgg acccgacatc
     7801 agctggtttt caactgcggt ctggatctcg ttgatatcca cctggttgcg cccctgcatc
     7861 tgctcgttga caaccgcggc aacagtggcg caataatcgg catcatcgac ttccgctgct
     7921 ttagctgcac gcagaatcgc ttctttgatg cgctctgatt taaacggcac tttgcagccg
     7981 tctcgtttca tcacatgcgg tgtcatgatc gctccatatt tttaagaaca ggttatccac
     8041 agaaattggg aaaggcgttt tccggttttt tcgtttcgtt ttccggtgct ttccgcaatc
     8101 gccatccgct tatccacaac gccgcacctt tttcgtgggg agctgtagta gcaattatag
     8161 tcgattaata caacatattg ggttgggacg cattttaaag tctatatata gtgctttgca
     8221 tcaaggatgt tttaactttt tttgatgtag ctcaaagtaa aaagcagagc gtacggatga
     8281 cgggcgctac agcgttatgt aaatttttta atgaatttgc tggttgaaaa atcaacaaaa
     8341 acaacatgct gacagacaaa accccggaat gaccggcatt atcggggctt agggaagatt
     8401 tacttctgta accaccagac agcctcaaaa ggccgtaaat tcatggcaca gggttgtggt
     8461 gaggcttctt cgtagttatg catcacaagc tgccagttgc cgcgcatttg ccctggctgc
     8521 cagggttgga tctcacggct aaggttggca atgaccagca aggtttgccc cttccattca
     8581 cggcgatagc accacaatac agggctgttt ggcagcagat cctggtaatt gccccatgtc
     8641 aggatggctt cttgcttacg cagtgcgatt aacttttggt aggtgtaaaa cacagaggag
     8701 tcatcggcca gcgcggcttc tacgttgatt tgttgatagt tatcgcctag gccaatccac
     8761 ggttcgccag ccgtaaaccc ggcattatcg ccgttgctcc attgcatggg cgtgcggctg
     8821 ttgtcacggg atttactggc gaggattgcc aataactcgt cggcatcacg accatcgttg
     8881 cgcagctcgg caaacatatt gaggctctcc acgtcgcgat agtcagtaat gcgcgtgaaa
     8941 tgcgggttgg tcatgccaat ctcttcgccc tggtagatat acggcgttcc ctgcatgcca
     9001 tgcagcacca tcgccagcat ttttgccgca ggcacgcggt attcaccttc atcaccaaag
     9061 cgagaaacaa tgcgcggctg atcgtggtta caccagaaca aggcattcca tgccacgttg
     9121 tgcattcctt gttgccagtg gcggaacaat gttttcaacg ccacaaagtc aggtttagcc
     9181 agcgtccatt tttcaccacc gggataatcg accttcaggt gatgaaaatt aaaggtcatc
     9241 gacaattcac tgcctgtcag tgccgcgtat cgctggcaat gctcaaggct ggtggaggac
     9301 atttcaccta cggtcattaa cccgcgtggc gtaaacacat cgcggttcat ctcgtgcaaa
     9361 aactcgtgtg ctcgtggccc gtcggtgtag aagcgacgcc cgtcgccatc caggtcttca
     9421 gggaaacgcg ggtctttgga gatcagattc accacatcca ggcgcaaccc gtcgacccca
     9481 cgatcggccc agaattcaca gacttttttc agctctgcgc gtaccgctgg attctcccag
     9541 ttgagatccg cctgttctgg tgcaaagaga tgcaaatagt actgttcgct ttccgcatgc
     9601 cagcgccacg cactaccgcc aaattttgaa cgccagttgt tcggtggcgt ttctggttcg
     9661 ccatcgcgcc agatataaaa ctggcggtaa gggctttctt tgttcagcgc ctcgcgaaac
     9721 caggcatgtt gggtagaggt atggttaaac accatatcga gaatgatacg aatcccgcgc
     9781 gattttgcct gcgtcaccag ttcgtcaaaa tcgtccagcg tgccgtaggt gggatcaatc
     9841 gccgtatagt tcgctacatc gtaaccgtta tcgacctggg gagagacata aaagggggtt
     9901 agccagatgg catcaacgcc cagtttatgc agatagtcca ggcgttggat aacgccacgt
     9961 aaatcgccgg tgccgctacc cgtggtgtcc tgaaaactct ttggataaat ctggtagata
    10021 acgccgtttt gccaccagtg gggaagatta gtcattacgt tattcctgca aatgcgaggg
    10081 ggcgcaattg cgccccgaag aaaattaaac aatgtccagc gtgcccaggc ggtatttccg
    10141 ctgatagata aacgaggtga gtacaatcgg gatgatgatg gcgatagcca ttgccagcgc
    10201 aaatacctgc cagtagctcg gttgaataga gagaattccc ggcaggcccc ctacgccgat
    10261 accattcgcc ataacgccgt tcaggccgca tagcaatcct gccagaccag aaccaatcat
    10321 cgcgcacagc atcgggaagc gatatttcag gttgatgccg tacattgcag gctcagtgac
    10381 cccaagccag gcggagatag cggcaggcac ggagatctcg cgttcattgt gcttgcggct
    10441 ggaaatgata atgcctatca cggcggagcc ctgagcgata ttcgacagcg caatcagcgg
    10501 ccacactggc gtgccaccca tgctttgaat catctgcaaa tcaatagcaa gggtggtctg
    10561 gtgtacgccg gtgatcacca gcggcgcgta caggaagcca aacaatgccg caccaatcgg
    10621 agcaaagctg ccggtcatca ggtgacgtac cgcaaaggca acgccatcgc caatcatgcg
    10681 accaaacgga ccaatcagcg catgggcgag gaacaccgcg aggatcagcg aacagacggg
    10741 caccaccacc agatagaggt aatccggtac gatgcgttta aggcgagttt caataacgcc
    10801 cagcgccagc ccggctaaca gtgccgggat cacctgcgcc tgatagccca ctttggcgat
    10861 gctgaacatg ccaaagtccc acacttccgg cagctgctgc ccgagcagat aagcgttcat
    10921 cagctggggg gaaaccagtg tcacgccaag cacgatacca aggatcggcg tgccgcccat
    10981 ttttttcacc gctgaccagc aaatcccgac cggcaggtag aagaaaatcg cttcaccgat
    11041 caaccacaga aaatcgtaga tcgtttgcag ggaagggtac atttgcgcca gcgtctgacc
    11101 gttgctcatg ggcaaatcgc cgatcacatt gcgaaaaccg aggatcaaac cgccgctaat
    11161 caacgcgggc agcaacggga agaagatctc cgcgaaatga gagatcaact gctcatgcca
    11221 tttcatattt tgccgggcgg ctttttttac ctgctcttta tcaacctgcg cctgtccggt
    11281 tgacgctatt agtgcttgat agtaatcacc cacgttggtg ccaatcacca cctgaaattg
    11341 cccggcattg gtgaaacagc ctttcaccat ggggagttgc tcaatttctt tcggtctggc
    11401 attggccggt tggttgagga caaagcgcag gcgagtaata cagtggctca ccgtcgcaat
    11461 attgccgcgc ccgccgacca gttcaatcaa ccgatcgata tccgtttggt ttattttgct
    11521 catcataaag ccccatggca gatgacattt ttggttggct gcagaatata ttgcgcggaa
    11581 aaatttaaaa acgggaacgt tcccgaaacg cagcgaagat cacaatttat cgttcaggaa
    11641 acgatcagga cagggtggcg gggatgatga tttgttgcgg ttcgctgcgc ccggttacct
    11701 gtgcgatcaa ttggcaggcc gcctggcgtc cagcttcggc gtagccagga tctacggtga
    11761 cgatctccgg atgaaggaat ttcattaacg gcgtattacc tacgctcgcc agttgcaagg
    11821 tgtcgatgcg ttgctcttgc aggtatttac ttgcgccaag agccagcgtg tcggttgcgc
    11881 acagtaacgc ggtagtttca ggcgtaatca cttttgctac gttttcatag ccttgcttca
    11941 tagcaagccc tggcagagcg gcaacgggat gcagtttatg cgctttgcag aacgccaggt
    12001 aggcttcgtg acgtcgcttc ccggtcgtca cgtcactatg cggcacgccg agataactga
    12061 tattgcgatg cccctggtca tagagccgtt gcatcagaat cttgattgcc ccttcgtcgt
    12121 cataacagac cgaagcaaag ccttttgcgt cacgcgccag cagaaccagc gatgactgcc
    12181 agtgggctaa catttcttcg gttatgccgg taaaaccgaa cagcactacg ccgtcgatat
    12241 tacgccgttt cagcaccccc aaatgttcgg caactaattg tggggaaaac tgattttcca
    12301 tcatgattgg atcgtaacct tgttcataga acgctggcag catggtttga acggcgagat
    12361 tttctgacaa cgaatccaga cgggtaacaa tgatggcgac cactttatcg ctttgcccac
    12421 gcatagcgcg cgcagagcgg gaaggggaaa atccatgctg attcatcact gcttcaacac
    12481 gctcgcgcgt gcgctgactc acgccgcttt cgttattcag cacccgggaa accgtagatt
    12541 tccccacgcc gcttaagcgc gcgatgtctt tgatggtcag ccgattttgc atcctgttgt
    12601 cccataacgt gttgtttaat tatttgagcc taactttacc cgtgcattca gcaatgggta
    12661 aagtctggtt tatcgttggt ttagttgcca gcaggtatta tatcgccata aatgctacga
    12721 atattattgg attctcctta ttatttgcgg cgcttttttc acttaccgga ggttatatgg
    12781 aacctgatcc cacgcctctc cctcgacgga gattaaaact tttccggtaa gcccgtcttt
    12841 tcacggcgtt accggatgcg taaggccgtg acgttttaat gtccctgctc agctttatta
    12901 ccttcaggta aggcttcgcc acgcctgaag acatttctgt actgtttcag acagtgcgga
    12961 gggactcctt atgtttaaag aaatttttac ccggttcatt cgccatttac cttcccgtct
    13021 ggttcatcgc gatccattgc ctggcgcgca gcagacagtg aatacggcgg tcccgccgtc
    13081 cttaagtgcg cattgcctga aaatggcggt gatgcccgaa gaagaattgt ggaaaacgtt
    13141 cgacacccat ccggaaggat taaatcaggc ggaagtggaa tctgcccgcg aacaacatgg
    13201 tgataataaa ttacccgccc aacaaccgtc gccgtggtgg gtacatttat gggtctgcta
    13261 tcgcaacccc tttaatattt tgctcaccat tcttggtgct atttcttacg ccacggaaga
    13321 tttatttgcc gcaggtgtta tcgcgctaat ggtcgctatt tctacgttgc tgaactttat
    13381 tcaggaagct cgttccacca aagcggcaga tgccctgaaa gcgatggtca gtaataccgc
    13441 gacggtactg cgagtaatta acgacaaagg cgaaaatggc tggctggaga tcccgataga
    13501 ccagctggtg cccggcgata ttataaaact ggcggcgggg gatatgatcc cggcagattt
    13561 acgtatcttg caggcgcggg atctgttcgt cgctcaggcg tcgttaaccg gtgagtctct
    13621 gcccgtggaa aaagccgcta ctactcgcca gccggagcac agcaatccgc tggagtgcga
    13681 caccctgtgt tttatgggca ccaccgtggt gagcggcacg gcacaagcaa tggtgattgc
    13741 tacaggagcc aacacctggt ttggtcaact ggcggggcgt gttagtgagc aggaaagcga
    13801 gccgaatgcc tttcagcaag ggatcagccg cgttagtatg ctgctgattc gctttatgct
    13861 ggtgatggct ccggtggtgt tgcttatcaa cggttacacc aaaggcgact ggtgggaagc
    13921 ggcactgttt gcgctttcgg tcgcggtagg cttaacgccg gaaatgttgc cgatgattgt
    13981 tacctcgacg ctggcgcgcg gcgcagtaaa gctgtcgaaa cagaaagtga tcgtcaaaca
    14041 tctggatgcc attcagaact ttggcgcaat ggatattctg tgcactgata aaaccggcac
    14101 cctgacgcag gacaaaattg tgctggagaa tcataccgat atctccggta aaaccagcga
    14161 acgcgtgctg catagcgcgt ggttgaacag tcattaccag accggactta aaaatctgct
    14221 tgataccgcg gtgctcgaag gtacggatga agagtcagcg cgttcgctgg ccagccgttg
    14281 gcaaaaaatt gatgagattc cgtttgattt cgagcgtcgt cggatgtcgg tggtagtggc
    14341 agaaaatacc gaacaccatc agctggtttg caaaggtgca ttgcaggaaa tcctcaatgt
    14401 gtgttcgcag gtgcgtcaca atggcgagat tgtaccgctc gatgacacca tgctgcgtaa
    14461 gattaagcgg gttactgata cgctgaatcg tcaggggctg cgcgtggttg cggtggcgac
    14521 gaaatacctg ccagcgcgtg aaggagatta ccagcgggcg gatgaatccg acctgatcct
    14581 cgaaggatat attgctttcc ttgatccgcc aaaagagaca accgctccgg cactgaaggc
    14641 attaaaagcg agtggaatta ccgtaaaaat ccttactggc gacagtgagt tagttgcagc
    14701 gaaagtgtgc catgaagtgg ggctggatgc gggagaggtg gtcattggta gtgatattga
    14761 aacgctatct gacgatgaac tggcaaatct cgcacagcgc accacgctgt ttgcccgcct
    14821 gacgccgatg cataaagaac gcattgtgac tttactgaag cgcgaagggc atgtggttgg
    14881 ctttatgggc gatggtatta atgatgcgcc cgccttacgc gctgcggata tcggcatttc
    14941 tgtggacggc gcggtagata ttgcccgtga agcggctgat atcatcctgc tggaaaaaag
    15001 cctgatggtg ctggaagagg gggttattga gggacgtcgc actttcgcca acatgctgaa
    15061 atacatcaaa ttgacggcga gttctaactt cggtaatgtg ttcagcgtgc tggtggcgag
    15121 tgctttcttg cccttcctgc cgatgttgcc gttacacttg cttattcaga acctgctgta
    15181 cgatgtgtca caggtggcga tcccgtttga taacgtcgac gacgagcaaa ttcaaaagcc
    15241 gcagcgttgg aatccggcgg atctggggcg ctttatggtc ttcttcggac cgatcagctc
    15301 gattttcgat attctgacgt tttgcttgat gtggtgggta ttccatgcca acacgccgga
    15361 aacgcaaacg ctgttccagt cgggctggtt tgtggtggga ttactgtcgc aaacgctgat
    15421 tgtgcatatg atccgtaccc gccgtgtgcc gtttattcag agctgtgcat cgtggccgtt
    15481 aatgatcatg accgtgatcg tgatgattgt cgggatcgca ttgccgtttt caccgctggc
    15541 cagttatctg cagctgcagg cgctgccgtt aagctatttc ccgtggctgg ttgcgattct
    15601 ggcagggtat atgacgttaa cccagttggt gaaagggttc tatagccgtc gttacggctg
    15661 gcagtaaaga ataaactggg catggtggcc cggattcaga ttgcagacaa agtgagcttt
    15721 gttcatgccg gatgcggcgt aaacgcccaa tccggcctac aaattcaata aattgcagga
    15781 atcacgtagg cctgataagc gtagcgcatc gggcgatttt gcttttgtca tcagacttaa
    15841 tccgggcatg atagcccgga tttccatcaa gattagcgac gaacagcgat tgcttcgatc
    15901 tcaatcttca cgtctttcgg cagacgggca acttcaacgc aagaacgtgc cgggaaagtg
    15961 gcgttgtgtt cggtgaagaa ggcttcgtaa gtggcgttta cggttgcgaa gtcgttcaga
    16021 tctttcacga acacagtggt tttaacgatg tcgcccactt tcaggccagc ggcttcgacg
    16081 attgctttta cattatccag cgactgacgt gcctgtgcag cgacgtctgc cggtacttca
    16141 ccagttttcg gatttaccgg gatctgaccg gaggtgatga tcatattgcc cagatcaacg
    16201 ccctgtacgt aaggaccgat agctgccggt gcattttccg tcgcgatagt tttgctcatg
    16261 atttctcctt tattacagcg gtaaaaagtc tggctcatta tagggagcca gacttttatt
    16321 accaaccgca attaattggc cagcaccaca ttatgggaaa actctttttc acagtatttg
    16381 catttaagcg cgatgtcatt ggcgcgtttt cgcacggcaa agctggatga aaccggttcg
    16441 gcatggctga tacagttgct gttcgggcag accagcacat tgtcgatgcg ctctggcaga
    16501 cttgggcgcg atttacccac cacttcatag ttgtcgatac ggttaaccgt ggcttgcggt
    16561 gcatacaatg ccagttggtc tacttgctct tcactcaaaa aggtattttc gattttgatc
    16621 agatctttgc ggcccatctc gccagaaggc aaattcagac caatggtgat gcgctgatct
    16681 gtttcggtca acttgaacag actcaacagc ttaaaaccga tctgggcggg gatatggtca
    16741 attaccgtgc cgcgtttaat agcttcaacc tgcaatttat tatcgtgtgt catctctatt
    16801 tccccttaca gtaccagatc gcgattcaga accagtgcca gtaacgcctg gcgagcgaaa
    16861 atcccgttgc ctgcttgctg gaagtaccag gcgtgaggcg ttttatccac atccgtcgca
    16921 atctcatcaa cacgcggcag cggatgcagc actttcatat tggctttggc gttgtggaga
    16981 tcgctggcgc gaagaacaaa ctgcgctttc acgttggcgt actcggacgg gtccagacgc
    17041 tctttttgca cgcgggtcat gtacaggatg tctacttccg ccatcacttc ttcaatagag
    17101 ctgtgcagac tccaggcgat ccctttttca tcgagcatat ccagaatgta ttgcggcatt
    17161 gccagcgcat ccggcgcgat aaagtaaaaa cggttgccgt cgaacttcgc tagcgcctgg
    17221 gtcagggagt gaacggtgcg gccatatttc aggtcaccaa ccattgcgac gtggagattg
    17281 tccagacgcc cctgggtttc ctgaatggtg aataagtcca gcaaggtttg cgtcggatgt
    17341 tggttggagc catcaccggc attcagtacc ggtacattgc cagaaaattc ggtggctagg
    17401 cgtgccgcac cttcctgcgg atgacgcatc actatcgcat cgacgtaagt gctgataacc
    17461 gaaatggtat cggccagcgt ttcgcctttt ttacccagtg atgtattggc gctgtcggag
    17521 aagcccacca cgctggcacc caggcggtgc atggaagttt cgaaagagag gcgggtacgg
    17581 gtagaggctt cgaagaaaca gctggcaatg actttgtgct tcaacagctc tggttgcggg
    17641 tttgctttca gtttcgccgc tgtcgccagc accagattaa ggtcatcgcg actaaggtcg
    17701 tttatggaaa tgatatgttt ctgatatagc ggattagcca tcttttatct cctgacacct
    17761 aggcaaaaaa aagcccctcg attgaggggc tgggaatggg tgatcaacgg gaagaaaaac
    17821 ggcaggccag cgtctttttt cagacgcggt aagacaaaat gtcgaacaca ctgaaccata
    17881 catcctcccg gcaaattgtc cggcattata ctcatagcca gaagcggtgc aagcatttga
    17941 tgcaatattt tgtcagcgca aacggtttat ttgaattaaa agtcaaaata tatgcatttt
    18001 tattttctat gattctggag gggaagtgtc gttcggtatc aataattgca gacatttcct
    18061 gcttttttct ttttcatttt cacgcaatca gaaatgcatt ttattccatt catccgactt
    18121 atagggcgga gggtgaaaac gaacggctaa cactattgct tatagctcag ggatacgcgc
    18181 tatcacttta atttcaaaat caaagcctgc cagccaggta acacccaccg ccgtccagtt
    18241 tggataaggt ggggcgctaa atatttcatt tttcaccgtc atgatgtctt cagattgttt
    18301 ttccggatcg gtatgaaagc tcgtaacatc aatgatatcg tcaaaagtgc atcccgcagc
    18361 tgccagggtc gcatgcaaat tatcaaaggc cagtctgact tgttgctgaa aatcgggttc
    18421 tggtgttccg tcctctcgac ttcctacttg cccggaaaca aacagcaaat cgccggaacg
    18481 aatagccgca gaataacgat gctcagcata cagtgaatgt cggccagcag ggaaaacagc
    18541 ggttctttct accatttggt tatcctcaag atttacgaca tgaacagaag atttatattt
    18601 atcgggagcc gcttttaggg ggacgacgtt agtaaacaaa acccagacat catggataat
    18661 ggctgggctt aattgagcgt agtcggttat gcgccaaacg cgccatcaat ggtatgcatc
    18721 gcgccggtaa caaaactggc ttctggccct gctaaccatg cgaccatacc agcgacctct
    18781 tccggttgcc catgtctttt gatggccata aaactatgca acatatcgcg cattggcccg
    18841 ttggcgggat taacgtcggt atcaattggt cctggctgga cgacgttgat ggtgatccct
    18901 cgcggtccaa aatcacgggc cagcccgcgc gccatgcctt gcagggcaga tttactggcg
    18961 gcataagcag ccatgcctgc aacaggcata cgatcgccat tcacggagcc gatgattaag
    19021 atgcgcccgc cttcgggcat ctgccgggcg gcttcaacag aggcatgata aggagcatga
    19081 atattgattt tgaaaaggcg atcaatatcg tcggcattta attccagggc atcgccaaag
    19141 acgccaatac ctgcatttac caccaggata tccaatgcgc cgctcttacg aacgacatca
    19201 ataacagcgt ctctgtcagc actatctgtg aatactgctg tcgctccagt ctcttgtgcc
    19261 agacgttcag cggcatcttt cgaccccgca taggtgaatc gtacattggc cccatcggtg
    19321 acgaaacgac gtacgatagc ggcaccgatt ccacgactgc caccgaggat gagaactgtc
    19381 ttaccggtaa aagcgcccat aaggactcct tgatttatta tgtaacatgc attacaaaac
    19441 tgttttaact ttctgtcaac aggttttgta atggtcacta aaaaacaatc tcgcgttcca
    19501 ggtcgtccca gacgtttcgc tcctgagcag gcagtctctg cggcaaaagt gctttttcac
    19561 caaaaaggtt tcgatgcagt aagtgtctct gaagttactg attatcttgg tattaacccc
    19621 ccgagtctct acgcggcttt tggcagtaag gctgggttat ttagccgtgt actcaatgaa
    19681 tatgtcggta cggaagctat tccgcttgcc gatattcttc gtgatgatcg tccggtaggc
    19741 gagtgcctgg ttgaggtatt aaaagaagcg gcgcgcagat atagccaaaa cggcggctgc
    19801 gctggctgta tggttcttga aggtattcat agtcatgatc cacaagcgcg tgatattgcc
    19861 attcaatatt atcacgccgc agaaacgacc atttatgact atatcgccag gcggcatcca
    19921 caaagcgcac aatgtgtgac tgattttatg agtaccgtga tgtcagggct ttctgcgaag
    19981 gcacgggagg ggcactccat cgaacaactc tgtgcaacag ctgccctggc aggggaagcg
    20041 ataaaaactc ttctcaagga gtgatgctgg ccttgatccg aaaggcggga acgcgcctgc
    20101 cgataagttg tgataagaca ataattcacg cgttaaggct ggcggaattg atcatctttt
    20161 cgtataacga tagaaatgaa acattgtttt aattaaggag tggaaaagat gatcatcgga
    20221 aatattcata atcttcagcc gtggctaccc caggagttac gccaggcgat tgagcatatc
    20281 aaagcacacg ttacggcaga aacgccaaag ggcaagcatg atatcgaagg caatcgtctg
    20341 ttttatctta tctcggaaga tatgaccgag ccgtacgaag ctcgccgtgc ggagtaccat
    20401 gcccgctatc ttgacattca gattgtgtta aaaggtcagg aaggcatgac cttcagcacg
    20461 caacctgcag gcgcgccgga taccgactgg ttagctgata aagacatcgc atttttgccg
    20521 gaaggcgttg atgagaaaac agttatccta aatgaaggtg attttgttgt gttttatccg
    20581 ggggaagtgc ataaaccgct gtgcgcagtg ggcgcgccag cccaggttcg caaagcagta
    20641 gtgaagatgc tgatggcgtg atgacttttc gccgtaaata actccaggtt tacggcgagt
    20701 ttgtgaaaag agcgtttttt gatatttttt tgtgagtaaa atttgtaatg cttagacgtt
    20761 cttattaact caaggagttc gtcatgggca aaatatcaga tttgaattat tctcaacaca
    20821 ttacattagc cgacaatttt aaacaaaaaa atgaagcctt agatacctgg tatgtgggaa
    20881 tgaatgattt tgcccgaatt gccggggggc agaatagcag aagcaatatt ctttctccta
    20941 gagcattttt ggagtttttg gcaaagatat ttaccctggg ttatgtggat tttagcaaac
    21001 gctccaacga agcgggtaga aatatgatgg ctcatattga gttctcatct tattctaaag
    21061 atactgatgg caatgaaaaa atgaagtttt acatgaataa tcctgaaggg gagcgagcgg
    21121 atttatctaa ggtgaagata gaaattacac ttgcctcagc ttctactaaa gggattcgtg
    21181 aaggacatac agtcattata tttaaacaat ctgatggttc gactaatcgt tatgaaggga
    21241 agtcttttga aagaaaagat gatagttcat tacacctgat tactaacaag gttctggcgt
    21301 gttaccaacg cgaagctaac aagaaaatag cgcgtctatt aaataatcat cagaagttaa
    21361 ataatataca ggagttaaat gattcgcagg agttaaataa ttcacagaag ttaaataatt
    21421 cgcaggagtt aaataactcg caggtgagtt gtaaaggttc agttgattct acgattacgg
    21481 atttattaga aaaagcattg aa
//
LOCUS       DAJTRC010000063        21330 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.063, whole genome shotgun sequence.
ACCESSION   DAJTRC010000063 DAJTRC010000000
VERSION     DAJTRC010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21330)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21330)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21330
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.063"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OO357_002592"
     CDS             complement(36..2105)
                     /gene="glyS"
                     /locus_tag="OO357_002592"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001291772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HCR5887499.1"
                     /translation="MSEKTFLVEIGTEELPPKALRSLAESFAANFTAELDNAGLAHGT
                     VQWFAAPRRLALKVANLAEAQPDREIEKRGPAIAQAFDAEGKPSKAAEGWARGCGITV
                     DQAERLTTDKGEWLLYRAHVKGESTEALLPNMVATSLAKLPIPKLMRWGASDVHFVRP
                     VHTVTLLLGDKVIPATILGIQSDRVIRGHRFMGEPEFTIDNADQYPEILRERGKVIAD
                     YEERKAKIKADAEEAARKIGGNADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEA
                     LVYTMKGDQKYFPVYANDGKLLPNFIFVANIESKDPQQIISGNEKVVRPRLADAEFFF
                     NTDRKKRLEDNLPRLQTVLFQQQLGTLRDKTDRIQALAGWIAEQIGADVNHATRAGLL
                     SKCDLMTNMVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVAC
                     ALAIADKMDTLAGIFGIGQHPKGDKDPFALRRAALGVLRIIVEKNLNLDLQTLTEEAV
                     RLYGDKLTNANVVDDVIDFMLGRFRAWYQDEGYTVDTIQAVLARRPTRPADFDARMKA
                     VSHFRTLEAAAALAAANKRVSNILAKSDEVLSDRVNASTLKEPEEIKLAMQVVVLRDK
                     LEPYFAEGRYQDALVELAELREPVDAFFDKVMVMVDDKELRLNRLTMLEKLRELFLRV
                     ADISLLQ"
     gene            complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OO357_002593"
     CDS             complement(2115..3026)
                     /gene="glyQ"
                     /locus_tag="OO357_002593"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HCR5887500.1"
                     /translation="MQKFDTRTFQGLILTLQDYWARQGCTIVQPLDMEVGAGTSHPMT
                     CLRALGPEPMAAAYVQPSRRPTDGRYGENPNRLQHYYQFQVVIKPSPDNIQELYLGSL
                     KELGMDPTIHDIRFVEDNWENPTLGAWGLGWEVWLNGMEVTQFTYFQQVGGLECKPVT
                     GEITYGLERLAMYIQGVDSVYDLVWSDGPLGKTTYGDVFHQNEVEQSTYNFEYADVDF
                     LFTCFEQYEKEAQQLLALENPLPLPAYERILKAAHSFNLLDARKAISVTERQRYILRI
                     RTLTKAVAEAYYASREALGFPMCNKDK"
     gene            complement(3121..3420)
                     /locus_tag="OO357_002594"
     CDS             complement(3121..3420)
                     /locus_tag="OO357_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YsaB family lipoprotein"
                     /protein_id="HCR5887501.1"
                     /translation="MMMNAFFPAMALMVLVGCSTPSPVQKAQRVRVDPLRSLNMEALC
                     KDQAAKRYNTGEQKIDVTAFEQFQGSYEMRGYTFRKEQFVCSFDADGHFLHLSMR"
     gene            3595..4590
                     /gene="wecH"
                     /locus_tag="OO357_002595"
     CDS             3595..4590
                     /gene="wecH"
                     /locus_tag="OO357_002595"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyltransferase WecH"
                     /protein_id="HCR5887502.1"
                     /translation="MQPKIYWIDNLRGIACLMVVMIHTTTWYVTNAHSVSPVTWDIAN
                     VLNSASRVSVPLFFMISGYLFFGERSAQPRHFLRIGLCLIFYSAIALLYIALFTSINM
                     ELALKNLLQKPVFYHLWFFFAIAVIYLVSPLIQVKNVGGKMLLVLMVVIGIIANPNTV
                     PQKIDGFEWLPINLYINGDTFYYILYGMLGRAIGMMDTQHKALSWVSAALFATGVFII
                     SRGTLYELQWRGNFADTWYLYCGPMVFICAIALLTLVKNTLDTRTIRGLGLISRHSLG
                     IYGFHALIIHALRTRGIELKNWPILDIIWIFCATLAASLLLSMLVQRIDRNRLVS"
     gene            complement(4632..5069)
                     /locus_tag="OO357_002596"
     CDS             complement(4632..5069)
                     /locus_tag="OO357_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YiaB family inner membrane protein"
                     /protein_id="HCR5887503.1"
                     /translation="MDNKISTYSPAFSIVSWIALVGGIVTYLLGLWNAEMQLNEKGYY
                     FAVLVLGLFSAASYQKTVRDKYEGIPTTSIYYMTCLTVFIISVALLMVGLWNATLLLS
                     EKGFYGLAFFLSLFGAVAVQKNIRDAGINPPKETQVTQEEYSE"
     gene            complement(5115..5456)
                     /locus_tag="OO357_002597"
     CDS             complement(5115..5456)
                     /locus_tag="OO357_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887504.1"
                     /translation="MKTSKTVAKLLFVVGALVYLVGLWISCPLLSGKGYFLGVLMTAT
                     FGNYAYLRAEKLGQLDNFFTHICQLVALITIGLLFIGVLNAPINAYEMVIYPIAFFVC
                     LFGQMRLFRSV"
     gene            complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="OO357_002598"
     CDS             complement(5625..7079)
                     /gene="xylB"
                     /locus_tag="OO357_002598"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HCR5887505.1"
                     /translation="MYIGIDLGTSGVKVILLNEQGEVVASQTEKLTVSRPHPLWSEQD
                     PEQWWQATDRAMKALGDQHSLQDVKALGIAGQMHGATLLDAQQRVLRPAILWNDGRCA
                     QECTLLEARVPQSRVITGNLMMPGFTAPKLLWVQRHEPEIFRQIDKVLLPKDYLRLRM
                     TGEFASDMSDAAGTMWLDVAKRDWSDVMLQACDLSRDQMPALYEGSEITGALLPEVAK
                     AWGMATVPVVAGGGDNAAGAVGVGMVDANQAMLSLGTSGVYFAVSEGFLSKPESAVHS
                     FCHALPQRWHLMSVMLSAASCLDWAAKLTGLCNVPALIAAAQQADESAEPVWFLPYLS
                     GERTPHNNPQAKGVFFGLTHQHGPNELVRAVLEGVGYALADGMDVVHACGIKPQSVTL
                     IGGGARSEYWRQMLADISGQQLDYRTGGDVGPALGAARLAQIAANPEKSLIELLPQLP
                     LEQSHLPDAQRYAAYQPRRETFRRLYQQLLPLMA"
     gene            complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="OO357_002599"
                     /pseudo
     CDS             complement(7151..8473)
                     /gene="xylA"
                     /locus_tag="OO357_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312475.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
     gene            8839..9831
                     /gene="xylF"
                     /locus_tag="OO357_002600"
     CDS             8839..9831
                     /gene="xylF"
                     /locus_tag="OO357_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5887506.1"
                     /translation="MKIKNILLTLCTSLLLTNVAAHAKEVKIGMAIDDLRLERWQKDR
                     DIFVKKAESLGAKVFVQSANGNEETQMSQIENMINRGVDVLVIIPYNGQVLSNVVKKA
                     KQEGIKVLAYDRMINDADIDFYISFDNEKVGELQAKALVDIVPQGNYFLMGGSPVDNN
                     AKLFRAGQMKVLKPYVDSGKIKVVGDQWVDGWLPENALKIMENALTANNNKIDAVVAS
                     NDATAGGAIQALSAQGLSGKVAISGQDADLAGIKRIAAGTQTMTVYKPITLLANTAAE
                     IAVELGNGQEPKADTSLNNGLKDVPSRLLTPIDVNKNNIKDTVIKDGFHKESEL"
     gene            9909..11450
                     /gene="xylG"
                     /locus_tag="OO357_002601"
     CDS             9909..11450
                     /gene="xylG"
                     /locus_tag="OO357_002601"
                     /EC_number="7.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter ATP-binding protein"
                     /protein_id="HCR5887507.1"
                     /translation="MPYLLEMKNITKTFGSVKAIDNVSLRLNAGEIVSLCGENGSGKS
                     TLMKVLCGIYPHGSYEGEIIFAGEEIQASHIRDTERKGIAIIHQELALVKELTVLENI
                     FLGNEITHNGIMDYDLMTLRYQKLLAQVSLSISPDTRVGDLGLGQQQLVEIAKALNKQ
                     VRLLILDEPTASLTEQETSVLLDIIRDLQQHGIACIYISHKLNEVKAISDTICVIRDG
                     QHIGTRDAAGMSEDDIITMMVERELTAIYPNEPHTTGDEILRIEHLTAWHPVNRHIKR
                     VNDVSFSLKRGEILGIAGLVGAGRTETIQCLFGVWPGQWEGKIYIDGKQVDIRNCQQA
                     ITQGIAMVPEDRKRDGIVPVMAVGKNITLAALNKFTGGISQLDDAAEQKCILESIQQL
                     KVKTSSPDLAIGRLSGGNQQKAILARCLLLNPRILILDEPTRGIDIGAKYEIYKLINQ
                     LVQQGIAVIVISSELPEVLGLSDRVLVMHEGKLKANLINHNLTQEQVMEAALRSEHHV
                     EKQSV"
     gene            11428..12609
                     /gene="xylH"
                     /locus_tag="OO357_002602"
     CDS             11428..12609
                     /gene="xylH"
                     /locus_tag="OO357_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xylose ABC transporter permease XylH"
                     /protein_id="HCR5887508.1"
                     /translation="MSKSNPSEVKLAVPTSGGFSGLKSLNLQVFVMIAAIIAIMLFFT
                     WTTDGAYLSARNVSNLLRQTAITGILAVGMVFVIISAEIDLSVGSMMGLLGGVAAICD
                     VWLGWPLPLTIIVTLVLGLLLGAWNGWWVAYRKVPSFIVTLAGMLAFRGILIGITNGT
                     TVSPTSAAMSQIGQSYLPASTGFIIGALGLMAFVGWQWRGRMRRQALGLQSPASTAVV
                     GRQALTAIIVLGAIWLLNDYRGVPTPVLLLTLLLLGGMFMATRTAFGRRIYAIGGNLE
                     AARLSGINVERTKLAVFAINGLMVAIAGLILSSRLGAGSPSAGNIAELDAIAACVIGG
                     TSLAGGVGSVAGAVMGAFIMASLDNGMSMMDVPTFWQYIVKGAILLLAVWMDSATKRR
                     S"
     gene            12687..13217
                     /locus_tag="OO357_002603"
     CDS             12687..13217
                     /locus_tag="OO357_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000494500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HCR5887509.1"
                     /translation="MFTKRHRITLLFNANKAYDRQVVEGVGEYLQASQSEWDIFIEED
                     FRARIDKIKDWLGDVVIADFDDKQIEQALADVDVPIVGVGGSYHLAESYPPVHYIATD
                     NYALVESAFLHLKEKGVNRFAFYGLPESSGKRWATEREYAFRQLVAEEKYRGVVYQGL
                     ETAPENWQHAQNRLAD"
     gene            13329..13865
                     /locus_tag="OO357_002604"
     CDS             13329..13865
                     /locus_tag="OO357_002604"
                     /inference="COORDINATES: protein motif:HMM:NF024242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HCR5887510.1"
                     /translation="MIGIDNEELTRYLSRVALSSVAQGARQMGYQAAKLLHRLLDKEE
                     MPLQRILVPPVRVIERRSTDYRSLTDPAVIQAMHYIRNHACKGIKVDQVLDAVGISRS
                     NLEKRFKEEVGETIHAMIHAEKLEKARSLLISTTLSINEISQMCGYPSLQYFYSVFKK
                     AYDTTPKEYRDVNSEVML"
     gene            complement(13973..14797)
                     /locus_tag="OO357_002605"
     CDS             complement(13973..14797)
                     /locus_tag="OO357_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein bax"
                     /protein_id="HCR5887511.1"
                     /translation="MILTPIRRYGAMILMLLTLVFSSEVLAKTHTTTASQKSHLTKAS
                     NKQVSSKQEYSRNSAKSSSLPDLRKYPSGTPRKKAFLRTVMPYITSQNAAITAERNWL
                     ISKQYQGQWAPAERARLKDIAKRYKVKWSGNTRKIPWNTLLERVDIIPTSMVATMAAA
                     ESGWGTSKLARNNNNLFGMKCMKGRCTNAPGKVKGYSQFSSVKESVSAYVTNLNTHPA
                     YSSFRKSRAQLRKADQEVTATAMIHKLKGYSTKGKSYNNYLFAMYQDNQRLIAAHM"
     gene            15117..17147
                     /gene="malS"
                     /locus_tag="OO357_002606"
     CDS             15117..17147
                     /gene="malS"
                     /locus_tag="OO357_002606"
                     /EC_number="3.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase"
                     /protein_id="HCR5887512.1"
                     /translation="MKLAACFLTLLPGFAVAASWTSPGFPAFSEQGTGTFVSHAQLPK
                     GTRPLTLNFDQQCWQPADAIKLNQMLSLQPCSNTPPQWRLFRDGKYTLQIDTRSGTPT
                     LMISIQNAAEPVANLVRECPKWDGLPLTLDVSATFPEGAAVRDYYSQQIAIVKNGQIT
                     LQPAATSNGLLLLERAETDTSAPFDWHNATVYFVLTDRFENGDPSNDQSYGRHKDGMA
                     EIGTFHGGDLRGLTNKLDYLQQLGVNALWISAPFEQIHGWVGGGTKGDFPHYAYHGYY
                     TQDWTNLDANMGNEADLRTLVDSAHQRGIRILFDVVMNHTSYATLADMQEYQFGALYL
                     SGDEVKKTLGERWSDWKPAAGQTWHSFNDYINFSDKTGWDKWWGKNWIRTDIGDYDNP
                     GFDDLTMSLAFLPDIKTESTTASGLPVFYKNKTDTHAKVIEGFTPRDYLTHWLSQWVR
                     DYGIDGFRVDTAKHVELPAWQQLKTEASSALREWKKTNPDKALDDKPFWMTGEAWGHG
                     VMQSDYYRHGFDAMINFDYQEQAAKAVDCLAQMDTTWQQMAEKLQGFNVLSYLSSHDT
                     RLFREGGDKAAELLLLAPGAVQIFYGDESSRPFGPTGSDPLQGTRSDMNWQDVSGKSA
                     ANVAHWQKISQFRARHPAIGAGKQTTLSLKQGYGFVREHGDDKVLVIWAGQQ"
     gene            17325..18578
                     /gene="avtA"
                     /locus_tag="OO357_002607"
     CDS             17325..18578
                     /gene="avtA"
                     /locus_tag="OO357_002607"
                     /EC_number="2.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--pyruvate transaminase"
                     /protein_id="HCR5887513.1"
                     /translation="MTFSLFGDKFTRHSGITLLMEDLNDGLRTPGAIMLGGGNPAQIP
                     EMQDYFQTLLTDMLESGKATDALCNYDGPQGKTELLTLLAGMLREKLGWDIEPQNIAL
                     TNGSQSAFFYLFNLFAGRRADGRVKKVLFPLAPEYIGYADAGLEEDLFVSARPNIELL
                     PEGQFKYHVDFEHLHIGEETGMICVSRPTNPTGNVITDEELLKLDALANQHGIPLVID
                     NAYGVPFPGIIFSEGRPLWNPNIVLCMSLSKLGLPGSRCGIIIANEKIITAITNMNGI
                     ISLAPGGIGPAMMCEMIKRNDLLRLSETVIKPFYYQRVQETIAIIRRYLPENRCLIHK
                     PEGAIFLWLWFKDLPITTEQLYQRLKARGVLMVPGHNFFPGLDKPWPHTHQCMRMNYV
                     PEPEKIEAGVKILAEEIERAWAESH"
     gene            complement(18729..19202)
                     /locus_tag="OO357_002608"
     CDS             complement(18729..19202)
                     /locus_tag="OO357_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5887514.1"
                     /translation="MNRFIIADATKCIGCRTCEVACAVSHQENQDCAALSPDEFISRI
                     RVIKDHSWTTPVACHQCEDAPCANVCPVDAISREHGHIFVEQTRCIGCKSCMLACPFG
                     AMEVVSSRKKARAIKCDLCWHRETGPACVEACPTKALQCMDVEKVQRHRLQQQPV"
     gene            complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="OO357_002609"
     CDS             complement(19304..20152)
                     /gene="yiaJ"
                     /locus_tag="OO357_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator YiaJ"
                     /protein_id="HCR5887515.1"
                     /translation="MGKEVMGKKENEMAQEKERPAGSQSLFRGLMLIEILSNYPNGCP
                     LAHLSELAGLNKSTVHRLLQGLQSCGYVTTAPAAGSYRLTTKFIAVGQKALSSLNIIH
                     IAAPHLEALNIATGETINFSSREDDHAILIYKLEPTTGMLRTRAYIGQHMPLYCSAMG
                     KIYMAFGHPDYVKSYWENHQHEIQPLTRNTITELPAMFDELAHIRESGAAMDREENEL
                     GVSCIAVPVFDIHGRVPYAVSISLSTSRLKQVGEKNLLKPLRETAQAISNELGFTVRD
                     DLGAIT"
     gene            20352..>21330
                     /gene="yiaK"
                     /locus_tag="OO357_002610"
     CDS             20352..>21330
                     /gene="yiaK"
                     /locus_tag="OO357_002610"
                     /EC_number="1.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydro-L-gulonate 2-dehydrogenase"
                     /protein_id="HCR5887516.1"
                     /translation="MKVTFEQLKAAFNRVLISRGVDSETADACAEMFARTTESGVYSH
                     GVNRFPRFIQQLENGDIIPDAQPKRITSLGAIEQWDAQRSIGNLTAKKMMDRAIELAA
                     DHGIGLVALRNANHWMRGGSYGWQAAEKGYIGICWTNSIAVMPPWGAKECRIGTNPLI
                     VAIPSTPITMVDMSMSMFSYGMLEVNRLAGRQLPVDGGFDDEGNLTKEPGVIEKNRRI
                     LPMGYWKGSGMSIVLDMIATLLSDGASVAEVTQDNSDEYGISQIFIAIEVDKLIDGPT
                     RDAKLQRIMDYVTTAERADENQAIRLPGHEFTTLLAENRRNGITVDDSVW"
BASE COUNT         5362 a         5443 c         5232 g         5293 t
ORIGIN      
        1 cctgccagat ggcaggcttt ttaataacgg cgttattact gcaacagcga aatatccgca
       61 acgcgcagga acagttcgcg cagtttctcc agcatggtca gacggttgag acgcaattct
      121 ttgtcatcaa ccatgaccat cactttatcg aagaaagcat caaccggctc acgcagctct
      181 gccagttcga ccagcgcatc ctggtaacga ccttccgcaa agtacggctc cagcttgtca
      241 cgtagcacca caacctgcat cgccagttta atttcttccg gctctttcag ggtagaggca
      301 ttcacgcgat cgctcagcac ttcgtcagat ttcgccagga tgttagatac acgtttgttc
      361 gccgccgcca gtgctgcagc tgcttccagg gtacggaagt gcgataccgc tttcatgcgg
      421 gcatcgaaat cagccggacg agtcggacga cgcgccagta ccgcctggat ggtgtcaacg
      481 gtataacctt cgtcctgata ccaggcgcgg aagcgaccga gcataaagtc gataacatca
      541 tcaactacgt tggcattagt cagcttatcg ccatacagac gcaccgcttc ttcggtcagc
      601 gtttgcagat caaggttgag gttcttctca acgataattc gcagcacgcc aagtgcggca
      661 cgacgcagcg caaacgggtc tttgtcgcct ttcggatgct gaccgatacc gaagataccc
      721 gccagagtat ccatcttatc agcaatcgcc agcgcacaag ctaccgggtt ggacggcagg
      781 tcatcaccgg caaagcgcgg ctgatactgc tcattcagcg cgacggctac gtcttccgct
      841 tcgccatcgt gacgcgcgta gtgcatcccc ataacgcctt gggtgtcggt gaactcgaag
      901 accatgttgg tcatcaggtc acacttagac agcaggcccg cacgggttgc gtggttaacg
      961 tcagcgccaa tctgttcggc gatccagcca gccagcgcct ggatgcggtc agtcttgtca
     1021 cgcagcgtac ccagctgttg ctggaacagc acggtttgca ggcgcggcag gttatcttcc
     1081 agacgttttt tgcggtcggt gttgaagaag aactcggcat ccgccagacg tggacgaacg
     1141 actttctcgt taccagagat aatttgctgc ggatctttcg attcgatgtt ggcaacgaag
     1201 ataaagttcg gcagcagttt gccgtcgttc gcatacaccg ggaagtactt ctggtcacct
     1261 ttcatggtgt aaaccagggc ttcagccggc accgcgagga atttctcttc gaattttgcg
     1321 gtcagaacga ccggccactc caccagcgaa gccacttctt ccagcaggct ttcgcttaag
     1381 tcagcgttac cgccaatctt acgcgctgct tcttcggcat cggctttaat cttcgcttta
     1441 cgttcttcgt aatcggcgat gactttcccg cgctcacgca gaatttccgg atactgatcg
     1501 gcgttgtcga tggtgaattc cggttcgccc ataaagcggt ggccgcgaat cacgcgatcg
     1561 gactgaatgc ccagaatggt tgccggaatg actttgtcgc ccagcagcag ggtcacggtg
     1621 tgcaccggac gcacgaagtg cacgtcgctt gcaccccagc gcatcagttt cgggatcggc
     1681 agtttcgcca gcgaagtcgc aaccatattc ggcagcagtg cttcggtgct ttcgcccttc
     1741 acatgggcgc gatacagcag ccattcgcct ttatcggtag tcagacgctc agcctggtca
     1801 acggtaatac cacaaccacg cgcccaacct tctgccgctt tgctcggttt tccttcagcg
     1861 tcgaacgcct gggcaatcgc cgggccgcgt ttttcgattt cacgatctgg ttgtgcttcc
     1921 gccaggttag ctactttcag cgccagacga cgcggagcag caaaccattg aacggtgccg
     1981 tgtgcgaggc ctgcgttatc cagctccgca gtaaagttcg cagcaaagga ctctgccagg
     2041 ctgcgcagtg cttttggtgg cagctcttca gtgccgattt ccaccagaaa agttttctca
     2101 gacatagccg cctcttactt atctttgttg cacatcggga agccgagggc ttcacgggaa
     2161 gcgtaatatg cttctgccac tgctttggtc agggtgcgaa tgcgcagaat gtagcgctga
     2221 cgctcggtga cggagatggc tttacgcgca tccagcaggt tgaagctgtg ggcggctttc
     2281 agaatacgct cgtaggctgg cagcggcagc ggattttcca gcgccagcag ctgctgcgct
     2341 tctttctcgt actgctcaaa gcaggtgaac aggaagtcca catccgcgta ttcgaagttg
     2401 taagtggact gctccacttc gttctgatgg aacacgtcgc cgtaggtggt tttacccagc
     2461 gggccgtcgc tccagactag gtcgtaaacg ctgtctacgc cctgaatgta catggccaga
     2521 cgttccagac cgtaggtgat ctcgccggta accggtttac actccagacc accaacctgc
     2581 tggaagtaag tgaactgcgt cacttccatg ccgtttagcc acacttccca gcccagtccc
     2641 caggccccca gagtcgggtt ttcccagtta tcttccacga aacggatgtc gtgaatagtc
     2701 gggtccatgc ccagctcttt cagagaaccg aggtacagct cctgaatatt gtccggcgat
     2761 ggcttaatga ccacctggaa ctgatagtag tgctgtaaac ggttggggtt ttcgccgtag
     2821 cgaccatcgg tcgggcgacg agaaggctga acataagcag ccgccatcgg ttctggcccc
     2881 agcgcgcgca gacaggtcat tgggtgagag gttcccgcgc cgacttccat gtccaatggt
     2941 tgaacaatgg tgcagccctg gcgagcccag taatcctgta aggtcaggat caagccctgg
     3001 aaggtcctgg tatcaaactt ttgcatatta tttcgtgctg gatacgtgtg gatttaaagg
     3061 aagggatcag tatacccgct ggatggaaga tatacagtac gaaacgggaa aaagcagggc
     3121 ttaacgcatg gaaagatgca aaaaatggcc atctgcgtca aaagaacaga caaactgctc
     3181 tttacggaag gtataaccgc gcatttcata gcttccctgg aactgttcga aggcggtgac
     3241 gtcgattttt tgctcgccgg tgttataacg ttttgccgcc tgatccttgc ataacgcttc
     3301 catattcaac gaacgcagag gatcaaccct tacccgttgt gctttctgca cgggtgacgg
     3361 tgtagaacaa cccactagca ccataagcgc cattgccgga aagaaagcgt tcatcatcat
     3421 taaattaccg tctggtttgc aggtgagtct tattatttat atcggtatag tggctgatat
     3481 cttacgaatt tccttaagcc tctataggct gtagtgataa aactcagatt attccagtgc
     3541 gccaaccaaa tgatttatcg gtcagacttt ttttaaccag atacagagac acagatgcag
     3601 cccaaaattt actggattga taacctgcga gggatagcgt gtttaatggt ggtgatgatt
     3661 cacaccacta cctggtatgt gaccaatgct catagtgtta gccccgtcac atgggatatc
     3721 gccaatgttc tgaattctgc ctctcgtgtc agcgtgccgc tatttttcat gatttccggc
     3781 tatctctttt ttggcgaacg cagcgcccag ccgcgccatt tcttgcgtat cggcttatgt
     3841 ctgatttttt atagcgcaat cgcactgctc tacattgcgc tgtttacctc catcaatatg
     3901 gagttagcgc tgaaaaacct gctgcaaaag ccagtgtttt accacttgtg gtttttcttc
     3961 gcgattgcgg tgatttatct ggtttcaccg ctgattcagg tgaagaacgt cggcggaaaa
     4021 atgttgctgg tgctaatggt ggtgattggc attatcgcta acccaaacac agtgccgcag
     4081 aaaattgacg gttttgaatg gctgccaatt aacttatata tcaatggcga tactttttac
     4141 tacattctgt atggcatgtt gggccgcgct atagggatga tggacacaca gcataaagca
     4201 ctgtcgtggg tgagcgccgc gctgtttgcg acgggggttt ttattatctc tcgcgggaca
     4261 ttatatgaat tgcagtggcg cggaaatttt gccgatacct ggtatcttta ctgtgggccg
     4321 atggttttta tctgcgcaat cgcgctattg actctggtta aaaacacgct ggatacgcgt
     4381 accattcgcg gacttggctt aatctcccgc cattcattgg gtatatacgg attccacgcc
     4441 ttgattatcc atgcgctgcg cacccgggga attgagctta aaaattggcc aatactggat
     4501 attatttgga tcttttgcgc gacgttggca gcgagtttgt tactttctat gctggtacaa
     4561 cgaatcgaca gaaacagatt agtgagttaa gtaaaagccc gttcacattg gactgaccgg
     4621 gcttacgtga attattcgct gtattcttcc tgggtaacct gtgtttcttt tggcgggttt
     4681 attccggcat cacgaatatt cttctgcacc gctactgcac caaacaagct taagaagaaa
     4741 gccagtccat aaaaaccttt ttcactgagt aataatgtcg cattccacag acctaccatc
     4801 agtagagcaa cagagataat aaagacagtc aggcaggtca tataataaat ggaagtggtc
     4861 ggtatgcctt catacttgtc ccgaacggtc ttttgataag acgcagcaga aaacagtcct
     4921 aacaccagta cggcaaaata ataccctttt tcatttaact gcatctctgc attccatagc
     4981 cctaacaggt aggtaacgat accaccaacg agagctatcc atgacacaat actaaaggcc
     5041 ggtgaatagg ttgatatttt gttgtccatc acagtattcc tttcattcct gaatgtgtaa
     5101 gagctttatg ttgctcatac cgagcgaaac aaacgcattt gaccaaacaa gcagacaaaa
     5161 aaggcgatgg ggtagatcac catttcataa gcattgatag gtgcatttaa aacaccgata
     5221 aacaagagac cgatagtgat taacgcaact aactggcaga tatgggtaaa aaaattatcc
     5281 agttgcccga gtttttctgc gcgaagatat gcatagttac caaaagttgc tgtcattaac
     5341 acgccaagaa aatagccttt tccacttagc aatgggcatg agatccatag cccaaccaga
     5401 taaaccagcg ccccgacaac aaataatagt tttgccacag tttttgaggt tttcataacg
     5461 atctccatat ctaccagcga tacattacga gtaaccaacg aaagacaaaa ctgaaaaatg
     5521 ccattaacaa atgattttca gaataaattc atactaaata ttaattaatt agtgagatat
     5581 atagatgtga attatccccc acccggtcag gcaggggata acgtttacgc cattaatggc
     5641 agaagttgct gatagaggcg acggaacgtt tcacgtcgtg gctgataagc ggcataacgc
     5701 tgcgcatctg gtagatgcga ctgttctaac ggtagctgcg gcaacaactc aatgagcgat
     5761 ttctctggat tcgccgcaat ctgcgccagc cttgctgcgc ccagtgctgg ccccacatcc
     5821 ccccccgtac ggtaatcgag ctgctgaccg ctgatatccg ccagcatctg acgccagtac
     5881 tcactacgcg ccccgccacc aatcaacgta acactttgcg gtttaatgcc gcaggcatgc
     5941 acgacatcca tgccatctgc cagcgcataa cccacgcctt ccagcactgc tcgcaccagt
     6001 tcattggggc catgttgatg agtcaaacca aagaaaaccc ccttcgcctg gggattattg
     6061 tgtggtgtac gctcgccgga aagataaggc agaaaccaaa ctggctcggc actttcatca
     6121 gcctgttgag ctgcagcgat taaagctggg acattgcaca ggccggttaa tttcgcggcc
     6181 caatccagac acgatgctgc actcagcatt acagacatta aatgccaacg ctgcggtagc
     6241 gcatggcaaa agctgtgtac agcgctttct ggcttgctta agaacccttc gctgacagca
     6301 aaatagaccc ccgacgtccc cagcgataac attgcctgat tagcatcaac cattcccaca
     6361 ccaactgcac cagctgcatt gtcgccaccg cctgcgacaa ctggcaccgt cgccataccc
     6421 cacgctttcg caacttcagg taacaaagca ccagtaattt cgctgccttc gtataatgcg
     6481 ggcatctggt cacgagataa gtcgcaagcc tgcagcatga cgtcactcca gtcacgcttt
     6541 gcgacatcca gccacatggt gccagctgcg tcagacatat cgctggcaaa ctcccccgtc
     6601 atacgcagac gcaagtaatc tttcggtaat aatactttgt cgatttgacg gaatatctcc
     6661 ggctcatgcc gctgaaccca tagcaattta ggcgcagtaa atccgggcat catcaggttg
     6721 ccggtaatca ctcgtgattg cggaactctc gcttccagca aagtgcactc ttgcgcacag
     6781 cgcccgtcgt tccacaaaat ggcagggcgc aatacccgtt gttgagcatc cagtaaggtt
     6841 gctccatgca tctggccggc aatacccaat gctttaacgt cctgcagaga atgctgatcg
     6901 cccagagctt tcattgcgcg atcagttgcc tgccaccact gttccgggtc ttgttccgac
     6961 caaagtggat gcgggcgcga aacggtcagc ttttccgttt gcgaagcaac cacctcaccc
     7021 tgctcgttga gcaaaataac ttttacgccc gaggtgccaa gatctatccc gatatacata
     7081 tcgatcgttc cttaaaaaaa tgcccggtat cgctaccgat aaccgggcca acggactgca
     7141 cagttagccg ttatttgtcg aacagataat gatttaccag attttccagt tgctcctggc
     7201 gaccactctg atgcaccgga gacaaattat gttcctgagc atatttggct aaatctgcca
     7261 gtgacatttg gcctttcagg atttgctggc ccaattcgct attccagccg gaataacgct
     7321 gcgcgatgcg tttatccagc tcgccatctt caatcatgcg cgctgcaatt ttcagcgcca
     7381 gtgccatcgt atccatcgcg ccgatatgac cgtaaaacag atcatattta tcagtacttt
     7441 gacgacgtac tttggcatcg aagttcagac caccggtggt gaaaccgcct gctttgagaa
     7501 tttcatacat caccagcgca ttctcttcca cactgttcgg gaactggtcg gtgtcccagc
     7561 ccagttgcgc atcgccacgg ttggcgtcga cagaaccgaa caggccaagc gcaatggcgg
     7621 tggctatttc atgatggaaa gagtgacctg ccagcgtcgc gtggttagct tcaatgttca
     7681 gtttaatctc tttttccaga ccaaactgtt tcaggaagcc atagaccgtc gcggcatcgt
     7741 aatcatattg atgtttagtc ggttcttgcg gtttcggttc gataagcaac gtgccctaga
     7801 agccgatttt atgtttatgc tcaaccacca tctgcataaa gcggcccagt tgttcacgct
     7861 cctgacgcaa gtcggtattt aacagcgttt cgtaaccttc acgaccaccc cacaggacat
     7921 agttttcacc gcccaattta tgggttgctt ccatcgctgt aacaacttgc gttgccgccc
     7981 agctgaagac ttcaggatct gggttcgtcg ccgcacccgc gccatagcga gggtttgtaa
     8041 agcagttagc ggttccccac agcagcttca cgccgctctc ttcttgcttg gctgccagga
     8101 catcaaccat ttgcgcaaaa ttattgatgt actcttttaa cgacgcgccc tcaggggaaa
     8161 catccacatc gtggaagcaa taaaatggca catgtaactt gtggaaaaac tcaaatgcga
     8221 catctgcttt acgcttcgcc aacgccagtg cctcaccagg ctgctgccac ggacgattaa
     8281 acgcccccac accaaacatg tccgccccgt tccagcagaa ggtgtgccag tagcaggcgg
     8341 caaaacgcaa gtgctcttcc atacgcttac ccaacaccag ttcgtcggga ttgtagtgac
     8401 ggaatgctaa cgggtttgag gattttgagc cttcataacg aacgcgatcg agctggtcaa
     8461 aataggcttg catattgaac tccataatca ggtaatgccg cgggtgatgg atgatgtcgt
     8521 aatattgggc actccctttc aattgctcaa ttatgttatt tcacactgct attgagataa
     8581 ttcacaagtg tgcgctcgct cgcaaaataa aatgggatga tgaaaatggg taattcctcg
     8641 aagataaaaa tgcaataagt acaattgcgc aacaaaagta agatctcggt cataaatcaa
     8701 gaaataaacc aaaaatcgta atcgaaagat aaaaatctgt aattgttttc ccctgtttag
     8761 ttgctaaaaa ttggttacgt ttatcgcggt gattgttact tattaaagct gtcctctaac
     8821 tacagaaggc cctacaccat gaaaataaag aacattctac tcaccctttg cacctcactt
     8881 ctgcttacca acgttgcggc acacgccaaa gaagtcaaaa taggtatggc gattgatgat
     8941 ctccgtcttg aacgctggca aaaagatcga gatatttttg tgaaaaaggc agaatctctc
     9001 ggtgcgaaag tatttgtaca gtctgcaaat ggcaatgaag aaacacaaat gtcgcagatt
     9061 gaaaacatga ttaaccgggg tgtcgatgtt cttgtcatta ttccgtataa cggtcaggta
     9121 ttaagtaacg ttgtaaaaaa agccaaacaa gaaggtatta aagtattagc ttacgaccgt
     9181 atgattaacg atgcggatat cgatttttat atttctttcg ataacgaaaa agtcggtgaa
     9241 ctgcaggcaa aagccttggt cgatattgtt ccgcaaggta attacttcct gatgggcggc
     9301 tcgccggtag ataacaacgc caagctgttc cgcgccggac aaatgaaagt gttaaaacct
     9361 tacgttgatt ctggaaaaat taaagtcgtt ggtgaccaat gggttgatgg ctggttaccg
     9421 gaaaacgcat tgaaaattat ggaaaacgcg ctaaccgcca ataataacaa aattgatgct
     9481 gtagttgcct caaacgatgc caccgcaggt ggggcaattc aggcattaag cgcgcaaggt
     9541 ttatcaggga aagtagcaat ttccggccag gatgcggatc tcgcaggtat taaacgtatt
     9601 gctgccggta cgcaaactat gacggtgtat aaacctatta cattgctggc aaatactgcc
     9661 gcagaaattg ccgttgaatt gggcaatggt caggagccaa aagcagatac ctcactgaat
     9721 aatggcctga aagatgtccc ctcccgcctc ctgacaccga tcgatgtgaa taaaaacaac
     9781 atcaaagata cggtaattaa agacggattc cacaaagaga gcgagctgta agcgttacgc
     9841 cccggcgcgg agcgggggcg tgatttctct ccatgccgcg tgaatgaatt ggcttaggtg
     9901 gagtcgttat gccttatcta cttgaaatga agaacattac caaaaccttc ggcagtgtga
     9961 aggcgattga taacgtcagc ttgcggttga atgctggcga aatcgtctca ctctgtgggg
    10021 aaaatgggtc tggtaaatca acgctgatga aagtgctgtg tggtatttat ccccatggct
    10081 cctacgaagg cgaaattatt tttgcgggag aagagattca ggcgagtcac atccgcgata
    10141 ccgaacgcaa aggtatcgcc atcattcacc aggaattggc cctggtgaaa gaattgaccg
    10201 tgctggaaaa tatcttcctg ggtaacgaaa taacccacaa tggcattatg gattatgacc
    10261 tgatgacgct acgctatcag aagctgctcg cacaggtcag tttatccatt tcacctgata
    10321 cccgcgttgg cgatttaggg cttgggcaac aacaactggt tgaaattgcc aaggcactta
    10381 ataaacaggt gcgcttgtta attctcgatg aaccgacagc ctcattaact gagcaggaaa
    10441 cgtcggtttt actggatatt attcgcgatc tacaacagca cggtatcgcc tgtatttata
    10501 tttcgcacaa actcaacgaa gtcaaagcga tttccgatac gatttgcgtt attcgcgacg
    10561 gacagcacat tggtacgcgt gatgctgccg gaatgagtga agacgatatt atcaccatga
    10621 tggtcgagcg agagttaacc gcgatttacc ctaatgaacc acataccacc ggagatgaaa
    10681 tattacgtat tgaacatctg acggcatggc atccggtcaa tcgtcatatt aaacgagtta
    10741 atgatgtctc gttttccctg aaacgtggcg aaatactggg tattgccgga ctcgttggtg
    10801 ccggacgtac cgagaccatt cagtgcctgt ttggcgtgtg gcccggacaa tgggaaggaa
    10861 aaatttatat tgatggcaaa caggtagata ttcgtaactg tcagcaagcc atcacccagg
    10921 ggattgcgat ggtacccgaa gacagaaagc gcgacggcat cgttccggta atggcggttg
    10981 gtaaaaatat taccctcgcc gcactcaata aatttaccgg tggcattagc cagcttgatg
    11041 acgcggcaga gcaaaaatgt attctggaat caattcagca actcaaagtt aaaacgtcgt
    11101 cccccgacct tgctattgga cgtttgagcg gcggcaatca gcaaaaagcg atcctcgccc
    11161 gctgtctgtt acttaacccg cgcatcctca ttcttgatga acctaccagg ggtattgata
    11221 ttggcgcgaa atatgagatc tacaaattaa ttaaccaact cgtccagcag ggtattgccg
    11281 ttattgtcat ctcttccgaa ttacctgaag tgctcggcct tagcgatcgt gtactggtga
    11341 tgcatgaagg gaaactaaaa gccaacctga taaatcataa cctgactcag gagcaggtga
    11401 tggaagccgc attgaggagc gaacatcatg tcgaaaagca atccgtctga agtgaaattg
    11461 gccgtaccga catccggtgg cttttctggg ctgaaatcac tgaatttgca ggtcttcgtg
    11521 atgattgcag ctatcatcgc aatcatgctg ttctttacct ggaccaccga tggtgcctac
    11581 ttaagcgccc gtaacgtctc caacctgtta cgccagaccg cgattaccgg catcctcgcg
    11641 gtaggaatgg tgttcgtcat aatttctgct gaaatcgacc tttccgtcgg ctcaatgatg
    11701 gggctattag gtggcgtcgc ggcgatttgt gacgtctggt taggctggcc tttgccactt
    11761 accatcattg tgacgttggt tctgggactg cttctcggtg cctggaacgg atggtgggtc
    11821 gcgtaccgca aagtcccttc atttattgtc accctcgcgg gcatgttggc atttcgcggc
    11881 atactcattg gcatcaccaa cggcacgact gtatccccca ccagcgccgc gatgtcacaa
    11941 attgggcaaa gctatctccc cgccagtacc ggcttcatca ttggcgcgct tggcttaatg
    12001 gcttttgttg gttggcaatg gcgcggaaga atgcgccgtc aggctttggg tttacagtct
    12061 ccggcctcta ccgcagtagt cggtcgccaa gctttaaccg ctatcatcgt attaggcgca
    12121 atctggctgt tgaatgatta ccgtggcgtt cccactcctg ttctgctgct gacgttgctg
    12181 ttactcggcg gaatgtttat ggcaacgcgg acggcatttg gacgacgcat ttatgccatc
    12241 ggcggcaatc tggaagcagc acgtctctcc gggattaacg ttgaacgcac caaacttgcc
    12301 gtgttcgcga ttaacggatt aatggtagcc atcgccggat taatccttag ttctcgactt
    12361 ggcgctggtt caccttctgc gggaaatatc gccgaactgg acgcaattgc agcatgcgtg
    12421 attggcggca ccagcctggc tggcggtgtg ggaagcgttg ccggagcagt aatgggggca
    12481 tttatcatgg cttcactgga taacggcatg agtatgatgg atgtaccgac cttctggcag
    12541 tatatcgtta aaggtgcgat tctgttgctg gcagtatgga tggactccgc aaccaaacgc
    12601 cgttcttgat tttgataaaa attttctcaa agccggttac gtattaccgg ttttgagttt
    12661 ttgcatgatt cagcaggaaa agaaccatgt ttactaaacg tcaccgcatc acattactgt
    12721 tcaatgccaa taaagcctat gaccggcagg tagtagaagg cgtaggggaa tatttacagg
    12781 catcacaatc agaatgggat attttcattg aagaagattt ccgcgcccgc attgataaaa
    12841 tcaaggactg gttaggagat gtcgtcattg ccgacttcga cgacaaacag atcgagcaag
    12901 cgctggctga tgtcgacgtc cccattgttg gggttggcgg ctcgtatcac cttgcagaaa
    12961 gttacccacc cgttcattac attgccaccg ataactatgc gctggttgaa agcgcatttt
    13021 tgcatttaaa agagaaaggc gttaaccgct ttgcttttta tggtcttccg gaatcaagcg
    13081 gcaaacgttg ggccactgaa cgcgaatatg catttcgtca gcttgtcgcc gaagaaaagt
    13141 atcgcggagt ggtttatcag gggttagaaa ccgcgccaga gaactggcaa cacgcgcaaa
    13201 atcggctggc agactgacta caaacgctgc caccgcaaac cgggattatt gccgttactg
    13261 acgcccgagc gcggcatatt ctgcaagtat gtgaacatct acatattccc gtaccggaaa
    13321 aattatgcgt gattggcatc gataacgaag aactgacccg ctatctgtcg cgtgtcgccc
    13381 tttcttcggt cgctcagggc gcgcggcaaa tgggctatca ggcggcaaaa ctgttgcatc
    13441 gattattaga taaagaagaa atgccgctac agcgaatttt ggtcccacca gttcgcgtca
    13501 ttgaacggcg ctcaacagat tatcgctcgc tgaccgatcc cgccgttatt caggccatgc
    13561 attacattcg taatcacgcc tgtaaaggga ttaaagtgga tcaggtactg gatgcggtcg
    13621 ggatctcgcg ctccaatctt gagaagcgtt ttaaagaaga ggtgggtgaa accatccatg
    13681 caatgattca tgccgagaag ctggagaaag cgcgcagtct gctgatttca accaccttgt
    13741 cgatcaatga gatatcgcaa atgtgcggtt atccatcgct gcaatatttc tactctgttt
    13801 ttaaaaaagc atatgacaca acgccaaaag agtatcgcga tgtaaatagc gaggtcatgt
    13861 tgtagcgatg atgagaattg tcggcgtcac atcagggaac tcagcacagc cacctgatgc
    13921 gagcaaaagc cttatccgac cgacaaatca gataaggctc gaggaaatga aattacatat
    13981 gcgccgcgat taaccgttgg ttatcctggt acattgcgaa caggtagttg ttataactct
    14041 tccctttggt cgagtagccc ttcagcttgt gaatcatcgc tgtggcagtc acttcctgat
    14101 ccgctttacg cagctgtgca cgcgatttac ggaacgaaga gtaagccggg tgcgtattca
    14161 ggttagtgac ataggcgctc accgattctt tgacagaact aaactgtgag taccctttca
    14221 ctttacccgg cgcattggta caacgtcctt tcatgcattt catgccgaac agattgttat
    14281 tgttgcgcgc cagcttcgac gttccccaac cgctttctgc tgcagccatc gtcgccacca
    14341 tactggtagg gataatgtct acgcgttcaa gcaaggtatt ccacgggatt tttcgcgtat
    14401 taccagacca cttcaccttg tagcgtttgg cgatgtcttt cagacgcgca cgctcagcag
    14461 gtgcccattg gccctgatac tgttttgaaa tcagccagtt acgttctgca gtaatggctg
    14521 cattttggct ggtaatgtaa ggcattacgg tccggagaaa cgcctttttc cttggtgttc
    14581 cggaagggta ttttcgcaaa tcaggaagtg aactactctt tgcactattg cgagaatact
    14641 cttgtttact gcttacctgt ttattactag ctttagttaa gtgggacttt tgactcgctg
    14701 ttgttgtgtg cgtcttcgct aacacctcac tcgaaaacac cagagtgagt aacataagaa
    14761 tcatcgcccc atatcgtcgt atgggagtca aaatcatcag gtctcctggt cggatttaat
    14821 cattccaaca ccttatattt ttcacaaatt tgagagttga atctcaaatc atatcaaaaa
    14881 tagctgtcaa gagcacccca aggaatagtc caaatctgaa actatgtcac gagttaacga
    14941 ttcagattgg cgctaaatcg cagaaaatgt gggggttatc gcaaaattca gccgtttttt
    15001 gcgcgcgatc gctcaccctt gcttctcatc ctgtggactt accgctcagg gatgagtttt
    15061 gtctggctta tcgctggcaa actgtctgaa atcgcagcaa taaggactca tccaccatga
    15121 aactcgccgc ctgttttctg acactccttc ctggcttcgc cgttgccgcc agctggactt
    15181 ctccggggtt ccctgccttt agcgaacagg gaacgggaac atttgtcagc cacgcgcagt
    15241 tgcccaaagg tacgcgtcca ctcacgctaa attttgacca acagtgctgg cagcctgcag
    15301 atgcgataaa actcaatcag atgctttccc tgcaaccttg tagcaacacg ccgcctcaat
    15361 ggcgattgtt cagggacggc aaatatacgc tgcaaataga cacccgctcc ggtacgccaa
    15421 cattgatgat ttccatccag aacgccgccg aaccggtagc aaacctggtc cgtgaatgcc
    15481 cgaaatggga tggattaccg ctcacgctgg atgtcagcgc cactttcccg gaaggagccg
    15541 ccgtacggga ttattacagc caacaaattg cgatagtgaa gaacggtcaa ataacgttac
    15601 aacccgctgc taccagcaac ggtttactcc tgctggaacg ggcagaaact gacacatccg
    15661 cccctttcga ctggcataac gccacggttt actttgtgct gacagatcgt ttcgaaaacg
    15721 gcgatcccag taatgaccag agttacggac gtcataaaga cggtatggcg gaaattggca
    15781 cttttcacgg cggcgattta cgcggcctga ccaacaaact ggattacctc cagcagttgg
    15841 gcgttaatgc tttatggata agcgccccat ttgagcaaat tcacggctgg gtcggcggtg
    15901 gtacaaaagg cgatttcccg cattatgcct accacggtta ttacacacag gactggacga
    15961 atcttgatgc caatatgggc aacgaagccg atctacggac gctggttgat agcgcacatc
    16021 agcgcggtat tcgtattctc tttgatgtcg tgatgaacca caccagctat gccacgctgg
    16081 cggatatgca ggagtatcag tttggcgcgt tatatctttc tggtgacgaa gtgaaaaaaa
    16141 cgctgggtga acgctggagc gactggaaac ctgccgccgg acaaacctgg catagcttta
    16201 acgattacat taatttcagc gacaaaacag gctgggataa atggtgggga aaaaactgga
    16261 tcagaacgga tatcggcgat tacgacaatc ctggattcga tgatctcact atgtcgctgg
    16321 cctttttgcc ggatatcaaa accgaatcaa ctaccgcttc tggtctgccg gtgttctata
    16381 aaaacaaaac ggatactcac gctaaagtca tcgaaggctt tacacctcgc gattacttaa
    16441 cccactggtt aagtcagtgg gtccgcgact atgggattga tggttttcgg gtcgataccg
    16501 ccaaacatgt tgagttgccc gcctggcagc aactgaaaac cgaagccagc tccgcgcttc
    16561 gcgaatggaa aaaaactaac cccgacaaag cattagatga caaacctttc tggatgaccg
    16621 gtgaagcctg gggccacggc gtgatgcaaa gtgactacta tcgccacggc ttcgatgcga
    16681 tgatcaattt cgattatcag gagcaggcgg cgaaagcagt cgattgtctg gcgcagatgg
    16741 atacgacctg gcagcaaatg gcggagaaat tgcagggttt caacgtgttg agctacctct
    16801 cgtcgcatga tacccgtctg ttccgtgaag ggggcgacaa agcagcagag ttattactat
    16861 tagcgccagg cgcggtacaa atcttttatg gcgatgaatc ctcgcgtccg ttcggtccta
    16921 caggttctga tccgctgcaa ggtacacgtt cggatatgaa ctggcaggat gttagcggta
    16981 aatctgccgc caacgtcgcg cactggcaga aaatcagcca gttccgcgcc cgccatcccg
    17041 caattggcgc gggcaaacaa acgacacttt cgctgaagca gggctacggc tttgttcgtg
    17101 agcatggcga cgataaagtg ctggtcatct gggctgggca acagtgactt ttccggcttc
    17161 ccgttcgtca gtacctcggg aagccgccaa ccaggataaa atgtcagccc taatcagcgt
    17221 tgcgggataa agcaccgctc actcttcaac agaccgattt gcaccccagc aaatgtagcg
    17281 ttattgttac cttccttgct acagagttcg acagatatcc cgctatgaca ttctcccttt
    17341 ttggtgacaa atttacccgc cactccggca ttacgctgct gatggaagat ctgaacgacg
    17401 gtttacgcac gcctggcgcg attatgctcg gcggcggtaa tccggcgcag atcccggaaa
    17461 tgcaggacta cttccagacg ctactgaccg acatgctgga aagtggcaaa gcgactgatg
    17521 cactgtgtaa ctacgacggt ccacagggga aaacggagct actcacactg cttgccggaa
    17581 tgctgcgcga gaagttgggt tgggatatcg aaccacagaa tattgcacta acaaacggca
    17641 gccagagcgc gtttttctac ttatttaacc tgtttgccgg acgccgtgcc gatggtcggg
    17701 tcaaaaaagt gctgttcccg ctggcaccgg aatacattgg ctacgctgac gccggactgg
    17761 aagaagatct gtttgtctct gcgcgtccga atattgaact gctgccggaa ggccagttta
    17821 aataccacgt cgattttgag catctgcata ttggcgaaga aaccgggatg atttgcgtct
    17881 cccggccgac gaatccaaca ggtaatgtga ttactgacga agagttgctg aagcttgacg
    17941 cgctggcgaa tcaacacggc attccgctgg tgattgataa cgcttatggc gtcccgttcc
    18001 cgggtatcat cttcagtgaa gggcgcccgc tatggaatcc gaatatcgta ctgtgcatga
    18061 gtctttccaa gctggggctg cctggctccc gctgcggcat tatcatcgcc aatgaaaaaa
    18121 tcatcactgc catcaccaat atgaacggca ttatcagcct ggcacctggc ggtattggtc
    18181 cggcgatgat gtgtgaaatg attaagcgta acgatctgct gcgcctgtct gaaacagtca
    18241 tcaaaccgtt ttactaccag cgtgttcagg aaactatcgc catcattcgc cgctatttac
    18301 cggaaaatcg ctgcctgatt cataaaccgg aaggagccat tttcctctgg ctatggttta
    18361 aggatttgcc cattacgacc gagcagctct atcagcgcct gaaagcacgc ggcgtgctga
    18421 tggtgccggg gcacaacttc ttcccgggac tggataaacc gtggccgcat acgcatcaat
    18481 gtatgcgcat gaactacgta ccagagccgg agaaaattga ggcgggggtg aagattctgg
    18541 cggaagagat agaaagagcc tgggctgaaa gtcactaaaa attgcctgat gcgctacgct
    18601 tatcaggcct acatgatttc tgcaatatat tgaatttgga agaatttgta ggccggataa
    18661 ggcgtttacg ccgcatctgg cataaacaaa gcgcactatt ccggcattga gaaacgccgg
    18721 aaaacgtttc aaacaggctg ctgctgtagc cgatgccgct gcactttctc gacatccatg
    18781 cactgcaacg cctttgtcgg gcaggcttca acacaggccg gccccgtctc ccgatgccag
    18841 cacagatcgc acttaatcgc cctcgccttt ttgcgcgaag aaacgacctc catcgcacca
    18901 aacgggcaag ccagcataca gcttttacag ccaatgcaac gtgtttgttc aacgaaaata
    18961 tgcccatgtt cgcggcttat cgcgtcaaca gggcagacat tcgcgcacgg tgcatcttca
    19021 cactgatgac aggctactgg cgtggtccag ctgtggtctt taatgacacg aatacgggaa
    19081 ataaactcgt ctggtgacaa cgcagcgcaa tcctgattct cctgatgcga cactgcgcaa
    19141 gccacttcac aggtacggca accgatacat ttcgtcgcat ccgcaataat aaaccggttc
    19201 atgatcattc ctttgcacta aaacagcaga gtgccagaga gcgcgtccct actgctttga
    19261 tccaacgaag gaaatgttaa agttttggca cttgtccaaa gcgttatgtg atcgcgccca
    19321 ggtcatccct gacagtaaat cccagttcat tagaaatagc ctgcgcggtt tcacgcagtg
    19381 gtttcaggag atttttctct cccacctgtt tcagacgtga tgtcgaaagc gaaatcgaca
    19441 cggcgtacgg cacccgccca tgaatatcaa acaccggaac agcaatacag gagacgccga
    19501 gttcgttttc ttctctgtcc atcgccgctc cgctttcacg aatgtgcgcc agttcgtcga
    19561 acatcgcggg cagctcggta atggtattgc gggttaacgg ctggatctca tgctgatggt
    19621 tttcccagta tgacttcacg tagtccgggt gaccaaacgc catgtagatc ttgcccattg
    19681 cggaacagta gagcggcata tgctggccaa tataggcacg ggttcgcagc atcccggttg
    19741 tgggttccag cttataaatc aaaatagcgt gatcgtcttc gcggctggag aagttaatgg
    19801 tttcaccagt ggcgatgttc agtgcctcaa gatgcggagc ggcgatatga atgatattca
    19861 gcgaagacag cgccttctgc ccgacggcaa taaatttggt agtcaggcgg taactccctg
    19921 cggcgggcgc ggtggtcaca tagccgcagg actgtaatcc ctgcaataag cgatggacgg
    19981 tactcttatt taaaccagcc agctccgaaa gatgcgccaa cggacaaccg tttggatagt
    20041 tgctcaaaat ctcaatcagc atcaacccgc gaaacagact ctggcttccg gctggacgct
    20101 ctttttcctg cgccatctcg ttctcttttt ttcccatcac ttctttcccc attttgtcgc
    20161 gtcctgatgg tagcgcaaag tgtgccgtag ttcacgatct cgacagataa tttataacca
    20221 cttgattttt atgtcttttg aaattcatca atcagattgc tttgttaaaa agtgatcgat
    20281 atatttgaaa tctagttccg catattgaaa ttttaagcca aaaaagcgat caaaaaacaa
    20341 ggaagcctag gatgaaagtg acatttgagc agttaaaagc agcctttaat cgggtcttaa
    20401 tttcacgcgg cgttgacagc gaaacggctg acgcctgtgc agagatgttc gcccgcacca
    20461 ccgaatccgg cgtttattct cacggcgtta atcgtttccc tcgtttcatt caacaactgg
    20521 aaaacggcga tatcattcct gatgcccaac ccaaacgtat aaccagcctc ggcgcaattg
    20581 aacagtggga tgcccagcgt tcgatcggca acctgacggc gaaaaagatg atggatcgcg
    20641 ccattgaact ggctgccgat cacggtattg gtctggtggc acttcgtaat gccaaccact
    20701 ggatgcgcgg cggcagctac ggctggcagg cggcggaaaa aggctatatt ggcatttgct
    20761 ggaccaactc catcgccgta atgccgccgt ggggcgcaaa agagtgtcgc atcggcacta
    20821 acccgctgat cgtcgccatt ccttccacgc cgatcaccat ggtcgatatg tcgatgtcga
    20881 tgttctctta cggcatgtta gaagttaacc gcctggcagg ccgtcagctc ccggtcgatg
    20941 gtggctttga tgatgagggc aatttgacca aagaacctgg cgttatcgag aagaatcgcc
    21001 gcattttgcc gatgggctac tggaaaggtt ctggcatgtc gattgtgctg gatatgatcg
    21061 ctactctcct ttccgacggc gcatcggttg ccgaagtcac ccaggacaac agcgacgaat
    21121 acggcatttc acaaattttt attgccattg aagtggacaa gcttatcgac ggtcccaccc
    21181 gcgatgccaa gctgcaacgt atcatggatt acgttactac cgctgaacgc gctgacgaaa
    21241 accaggccat ccgcttaccc ggccacgaat ttactaccct gctggccgaa aaccgccgta
    21301 acggcatcac tgttgatgac agcgtgtggg
//
LOCUS       DAJTRC010000064        21329 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.064, whole genome shotgun sequence.
ACCESSION   DAJTRC010000064 DAJTRC010000000
VERSION     DAJTRC010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 21329)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21329)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21329
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.064"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            18..791
                     /locus_tag="OO357_002611"
                     /pseudo
     CDS             18..791
                     /locus_tag="OO357_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001445615.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YgcG family protein"
     gene            complement(851..2149)
                     /gene="eno"
                     /locus_tag="OO357_002612"
     CDS             complement(851..2149)
                     /gene="eno"
                     /locus_tag="OO357_002612"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HCR5887517.1"
                     /translation="MSKIVKIIGREIIDSRGNPTVEAEVHLEGGFVGMAAAPSGASTG
                     SREALELRDGDKSRFLGKGVTKAVAAVNGPIAQALIGKDAKDQAGIDKIMIDLDGTEN
                     KSKFGANAILAVSLANAKAAAAAKGMPLYEHIAELNGTPGKYSMPVPMMNIINGGEHA
                     DNNVDIQEFMIQPVGAKTVKEAIRMGSEVFHHLAKVLKAKGMNTAVGDEGGYAPNLGS
                     NAEALAVIAEAVKAAGYELGKDITLAMDCAASEFYKDGKYVLAGEGNKAFTSEEFTHF
                     LEELTKQYPIVSIEDGLDESDWDGFAYQTKVLGDKIQLVGDDLFVTNTKILKEGIEKG
                     IANSILIKFNQIGSLTETLAAIKMAKDAGYTAVISHRSGETEDATIADLAVGTAAGQI
                     KTGSMSRSDRVAKYNQLIRIEEALGEKAPYNGRKEIKGQA"
     gene            complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="OO357_002613"
     CDS             complement(2237..3874)
                     /gene="pyrG"
                     /locus_tag="OO357_002613"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase (glutamine hydrolyzing)"
                     /protein_id="HCR5887518.1"
                     /translation="MTTNYIFVTGGVVSSLGKGIAAASLAAILEARGLNVTIMKLDPY
                     INVDPGTMSPIQHGEVFVTEDGAETDLDLGHYERFIRTKMSRRNNFTTGRIYSDVLRK
                     ERRGDYLGATVQVIPHITNAIKERVLEGGEGHDVVLVEIGGTVGDIESLPFLEAIRQM
                     AVEIGREHTLFMHLTLVPYMAASGEVKTKPTQHSVKELLSIGIQPDILICRSDRAVPA
                     NERAKIALFCNVPEKAVISLKDVDSIYKIPGLLKSQGLDDYICKRFSLNCPEANLSEW
                     EQVIFEEANPVSEVTIGMVGKYIELPDAYKSVIEALKHGGLKNRVSVNIKLIDSQDVE
                     TRGVEILKGLDAILVPGGFGYRGVEGMITTARFARENNIPYLGICLGMQVALIDYARH
                     VANMENANSTEFVPDCKYPVVALITEWRDENGNVEVRSEKSDLGGTMRLGAQQCQLVD
                     DSLVRQLYNAPTIVERHRHRYEVNNMLLKQIEDAGLRVAGRSGDDQLVEIIEVPNHPW
                     FVACQFHPEFTSTPRDGHPLFAGFVKAASEFQKRQAK"
     gene            complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="OO357_002614"
     CDS             complement(4102..4893)
                     /gene="mazG"
                     /locus_tag="OO357_002614"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HCR5887519.1"
                     /translation="MNQIDRLLTIMQRLRDPENGCPWDKEQTFATIAPYTLEETYEVL
                     DAIAREDFDDLRGELGDLLFQVVFYAQMAQEEGRFDFNDICAAISDKLERRHPHVFAD
                     SSAENSSEVLARWEQIKTEERAQKAQHSALDDIPRSLPALMRAQKIQKRCANVGFDWT
                     TLGPVVDKVYEEIDEVMYEARQAVVDQAKLEEEMGDLLFATVNLARHLGTKAEIALQK
                     VNEKFERRFREVERIVAARGLEMTGVDLETMEEVWQQVKRQEIDL"
     gene            complement(4989..7223)
                     /locus_tag="OO357_002615"
     CDS             complement(4989..7223)
                     /locus_tag="OO357_002615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HCR5887520.1"
                     /translation="MVAVRSAHINKAGEFDPEKWIASLGITSQKSCECLAETWAYCLQ
                     QTQGHPDASLLLWRGVEMVEILSTLSMDIDTLRAALLFPLADANVVSEDVLRESVGKS
                     VVNLIHGVRDMAAIRQLKATHTDSVSSEQVDNVRRMLLAMVDDFRCVVIKLAERIAHL
                     REVKDAPEDERVLAAKECTNIYAPLANRLGIGQLKWELEDYCFRYLHPTEYKRIAKLL
                     HERRLDREHYIEEFVGHLRAEMKAEGVKAEVYGRPKHIYSIWRKMQKKNLAFDELFDV
                     RAVRIVAERLQDCYAALGIVHTHYRHLPDEFDDYVANPKPNGYQSIHTVVLGPGGKTV
                     EIQIRTKQMHEDAELGVAAHWKYKEGAAAGGARSGHEDRIAWLRKLIAWQEEMADSGE
                     MLDEVRSQVFDDRVYVFTPKGDVVDLPAGSTPLDFAYHIHSDVGHRCIGAKIGGRIVP
                     FTYQLQMGDQIEIITQKQPNPSRDWLNPNLGYVTTSRGRSKIHAWFRKQDRDKNILAG
                     RQILDDELEHLGISLKEAEKHLLPRYNFNDVDELLAAIGGGDIRLNQMVNFLQSQFNK
                     PSAEEQDAAALKQLQQKSYTPQNRSKDNGRVVVEGVGNLMHHIARCCQPIPGDEIVGF
                     ITQGRGISVHRADCEQLAELRSHAPERIVDAVWGESYSAGYSLVVRVVANDRSGLLRD
                     ITTILANEKVNVLGVASRSDTKQQLATIDMTIEIYNLQVLGRVLGKLNQVPDVIDARR
                     LHGS"
     gene            complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="OO357_002616"
     CDS             complement(7271..8572)
                     /gene="rlmD"
                     /locus_tag="OO357_002616"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HCR5887521.1"
                     /translation="MAQFYSAKRRTTTRQIITVSVNDLDSFGQGVARHNGKTLFIPGL
                     LPQENAEVAVTEDKKQYARAKVVRRLSDSPERETPRCPHFGVCGGCQQQHASVDLQQR
                     SKSAALARLMKHDVSEVIADVPWGYRRRARLSLNYLPKTQQLQMGFRKAGSSDIVDVK
                     QCPILAPQLEALLPKVRACLGSLQAMRHLGHVELVQATSGTLMILRHTAPLSSADREK
                     LERFSHSEGLDLYLAPDSEILETVSGEMPWYDSNGLRLTFSPRDFIQVNAGVNQKMVA
                     RALEWLDVQPEDRVLDLFCGMGNFTLPLATQAASVVGVEGVPALVEKGQQNARLNGLQ
                     NVTFYHENLEEDVTKQPWAKNGFDKVLLDPARAGAAGVMQQIIKLEPIRIVYVSCNPA
                     TLARDSEALLKAGYTIARLAMLDMFPHTGHLESMVLFSRVK"
     gene            8629..11385
                     /gene="barA"
                     /locus_tag="OO357_002617"
     CDS             8629..11385
                     /gene="barA"
                     /locus_tag="OO357_002617"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase BarA"
                     /protein_id="HCR5887522.1"
                     /translation="MTNYSLRARMMILILAPTVLIGLLLSIFFVVHRYNDLQRQLEDA
                     GASIIEPLAVSTEYGMSLQNRESIGQLISVLHRRHSDIVRAISVYDENNRLFVTSNFH
                     LDPSSMQLGSNVPFPRQLTVTRDGDIMILRTPIISESYSPDESPSSDAKNSQNMLGYI
                     ALELDLKSVRLQQYKEIFISCVMMLFCIGIALIFGWRLMRDVTGPIRNMVNTVDRIRR
                     GQLDSRVEGFMLGELDMLKNGINSMAMSLAAYHEEMQHNIDQATSDLRETLEQMEIQN
                     VELDLAKKRAQEAARIKSEFLANMSHELRTPLNGVIGFTRLTLKTELTPTQRDHLNTI
                     ERSANNLLAIINDVLDFSKLEAGKLILESIPFPLRSTLDEVVTLLAHSSHDKGLELTL
                     NIKSDVPDNVIGDPLRLQQIITNLVGNAIKFTENGNIDILVEKRALSNTKVQIEVQIR
                     DTGIGIPERDQSRLFQAFRQADASISRRHGGTGLGLVITQKLVNEMGGDISFHSQPNR
                     GSTFWFHINLDLNPNIIIEGPSIQCLAGKRLAYVEPNSAAAQCTLDILSETPLEVVYS
                     PTFSALPPAHYDMMLLGIAVTFREPLTMQHERLAKAVSMTDFLMLALPCHAQVNAEKL
                     KQDGIGACLLKPLTPTRLLPALTEFCHHKQNTLLPVTDESKLAMTVMAVDDNPANLKL
                     IGALLEDMVQHVELCDSGHQAVERAKQMPFDLILMDIQMPDMDGIRACELIHQLPHQR
                     QTPVIAVTAHAMAGQKEKLLGAGMSDYLAKPIEEERLHNLLLRYKPGSGISSRVVTPE
                     VNEIVVNPNATLDWQLALRQAAGKTDLARDMLQMLLDFLPEVRNKVEEQLAGENPEGL
                     VDLIHKLHGSCGYSGVPRMKNLCQLIEQQLRSGTKEEDLEPELLELLDEMDNVAREAS
                     KILG"
     gene            complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="OO357_002618"
     CDS             complement(11617..12957)
                     /gene="gudD"
                     /locus_tag="OO357_002618"
                     /EC_number="4.2.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5887523.1"
                     /translation="MSSQFTTPVVTEMQVIPVAGHDSMLMNLSGAHAPFFTRNIVIIK
                     DNSGHTGVGEIPGGEKIRKTLEDAIPLVVGKTLGEYKNVLTLVRNTFADRDASGRGLQ
                     TFDLRTTIHVVTGIEAAMLDLLGQHLGVNVASLLGDGQQRSEVEMLGYLFFVGNRKAT
                     PLPYQSQPDDSCDWYRLRHEEAMTPDAVVRLAEAAYEKYGFNDFKLKGGVLAGEEEAE
                     SIVALAQRFPQARITLDPNGAWSLNEAIKIGKYLKGSLAYAEDPCGAEQGFSGREVMA
                     EFRRATGLPTATNMIATDWRQMGHTLSLQSVDIPLADPHFWTMQGSVRVAQMCHEFGL
                     TWGSHSNNHFDISLAMFTHVAAAAPGKITAIDTHWIWQEGNQRLTKEPFEIKGGLVQV
                     PEKPGLGVEIDMDQVMKAHELYQKHGLGARDDAMGMQYLIPGWTFDNKRPCMVR"
     gene            complement(12978..14318)
                     /locus_tag="OO357_002619"
     CDS             complement(12978..14318)
                     /locus_tag="OO357_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucarate dehydratase"
                     /protein_id="HCR5887524.1"
                     /translation="MATQSSPVITDMKVIPVAGHDSMLLNIGGAHNAYFTRNIVVLTD
                     NAGHTGIGEAPGGEVIYQTLVDAIPMVLGQEVARLNKVVQQVHKGNQAADFDTFGKGA
                     WTFELRVNAVAALEAALLDLLGKALNVPVCELLGPGKQRETITVLGYLFYIGDRTKTD
                     LPYLENTPGNHEWYQLRHQKAMNSEAVVRLAEASQDRYGFKDFKLKGGVLPGEQEIDT
                     VRALKKSFPDARITVDPNGAWLLDEAISLCKGLNDVLTYAEDPCGAEQGFSGREVMAE
                     FRRATGLPVATNMIATNWREMGHAVMLNAVDIPLADPHFWTLSGAVRVAQLCDDWGLT
                     WGCHSNNHFDISLAIFTHVGAAAPGNPTAIDTHWIWQEGDCRLTKNPLEIKNGKIAVP
                     DAPGLGVELDWEQVQKAHEAYKRLPCGARNDAGPMQYLIPGWTFDRKRPVFGRH"
     gene            complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="OO357_002620"
     CDS             complement(14320..15672)
                     /gene="gudP"
                     /locus_tag="OO357_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactarate/glucarate/glycerate transporter
                     GudP"
                     /protein_id="HCR5887525.1"
                     /translation="MSSLSQAASSVEKRTNARYWIVVMLFIVTSFNYGDRATLSIAGS
                     EMAKDIGLDPVGMGYVFSAFSWAYVIGQIPGGWLLDRFGSKRVYFWSIFIWSMFTLLQ
                     GFVDIFSGFGIIVALFTLRFLVGLAEAPSFPGNSRIVAAWFPVQERGTAVSIFNSAQY
                     FATVIFAPIMGWLTHEVGWSHVFFFMGGLGIVISFIWLKVIHEPNQHPGVNQKELEYI
                     AAGGALINMDQQSTKVKVPFSVKWGQIKQLLGSRMMIGVYIGQYCINALTYFFITWFP
                     VYLVQARGMSILKAGFVASVPAVCGFIGGVLGGIISDWLMRRTGSLNIARKTPIVMGM
                     LLSMVMVFCNYVNVEWMIIGFMALAFFGKGIGALGWAVMADTAPKEISGLSGGLFNMF
                     GNISGIVTPIAIGYIVGTTGSFNGALIYVGVHALIAVLSYLVLVGDIKRIELKPVAGQ
                     "
     gene            complement(16106..16555)
                     /locus_tag="OO357_002621"
     CDS             complement(16106..16555)
                     /locus_tag="OO357_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HCR5887526.1"
                     /translation="MAEIGIFVGTMYGNSLLVAEEAEAILTAQGHKATVFEDPELSDW
                     LPYQDKYVLVVTSTTGQGDLPDSIVPLFQGIKDSLGFQPNLRYGVIALGDSSYVNFCN
                     GGKQFDALLQEQSAQRVGEMLLIDASENPEPETESNPWVEQWGTLLS"
     gene            complement(16573..17355)
                     /gene="truC"
                     /locus_tag="OO357_002622"
     CDS             complement(16573..17355)
                     /gene="truC"
                     /locus_tag="OO357_002622"
                     /EC_number="5.4.99.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(65) synthase TruC"
                     /protein_id="HCR5887527.1"
                     /translation="MLEILYQDEWLVAVNKPSGWLVHRSWLDRDEKVVVMQTVRDQIG
                     QHVFTAHRLDRPTSGVLLMGLSSEAGRLLAQQFEQHQIQKRYHAIVRGWLMEEAVLDY
                     PLVEELDKIADKFAREDKGPQPAVTHYRGLATVEMPVATGRYPTTRYGLVELEPKTGR
                     KHQLRRHLAHLRHPIIGDSKHGDLRQNRSGAEHFGLQRLMLHASQLSLTHPFTGEPLT
                     IHAGLDDTWMQALSQFGWRGLLPENERVEFSAPSGQDGEISS"
     gene            complement(17355..17684)
                     /locus_tag="OO357_002623"
     CDS             complement(17355..17684)
                     /locus_tag="OO357_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqcC family protein"
                     /protein_id="HCR5887528.1"
                     /translation="MTTHDRVRLQLQALEALLREHQHWRNDEPQPHQFNSTQPFFMDT
                     MEPLEWLQWGLIPRMHDLLNNNQPLPGAFAVAPYYEMALATDHPQRALILAELEKLDA
                     LFADDAS"
     gene            complement(18306..18851)
                     /gene="syd"
                     /locus_tag="OO357_002624"
     CDS             complement(18306..18851)
                     /gene="syd"
                     /locus_tag="OO357_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SecY-interacting protein"
                     /protein_id="HCR5887529.1"
                     /translation="MDDLTAQALKDFTARYCDAWHEEHKSWPLSEELYGVPSPCIIST
                     TEDAVYWQPQPFTGEQNVNAVERAFDIVIQPTIHTFYTTQFAGDMHAQFGDIKLTLLQ
                     TWSEDDFRRVQENLIGHLVTQKRLKLPPTLFIATLEEELEVISVCNLSGEVCKETLGT
                     RKRTNLASNLAEFLNQLKPLL"
     gene            18919..19767
                     /gene="queF"
                     /locus_tag="OO357_002625"
     CDS             18919..19767
                     /gene="queF"
                     /locus_tag="OO357_002625"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent 7-cyano-7-deazaguanine reductase
                     QueF"
                     /protein_id="HCR5887530.1"
                     /translation="MSSYANHQALAGLTLGKSTDYRDTYDASLLQGVPRSLNRDPLGL
                     KADNLPFHGTDIWTLYELSWLNAKGLPQVAVGHVELDYTSVNLIESKSFKLYLNSFNQ
                     TRFNNWDEVRQTLERDLSTCAQGKISVALYRLDELEGQPIGHFNGTCIDDKDITIDNY
                     EFTTDYLENATSGEKVVEETLVSHLLKSNCLITHQPDWGSIQIQYRGRQIDREKLLRY
                     LVSFRHHNEFHEQCVERIFNDLLRFCQPEKLSVYARYTRRGGLDINPWRSNSDFVPST
                     TRLVRQ"
     gene            19879..21243
                     /gene="ppnN"
                     /locus_tag="OO357_002626"
                     /gene_synonym="ygdH"
     CDS             19879..21243
                     /gene="ppnN"
                     /locus_tag="OO357_002626"
                     /gene_synonym="ygdH"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide 5'-monophosphate nucleosidase"
                     /protein_id="HCR5887531.1"
                     /translation="MITHISPLGSMDMLSQLEVDMLKRTASSDLYQLFRNCSLAVLNS
                     GSLTDNSKELLSRFENFDINVLRRERGVKLELINPPEEAFVDGRIIRALQANLFAVLR
                     DILFVYGQIHHTVRFPNLNLDNSVHITNLVFSILRNARALHVGEAPNMVVCWGGHSIN
                     ENEYLYARRVGNQLGLRELNICTGCGPGAMEAPMKGAAVGHAQQRYKDSRFIGMTEPS
                     IIAAEPPNPLVNELIIMPDIEKRLEAFVRIAHGIIIFPGGVGTAEELLYLLGILMNPA
                     NKDQVLPLILTGPKESADYFRVLDEFVVHTLGENARRHYRIIIDDAAEVARQMKKSMP
                     LVKENRRDTGDAYSFNWSMRIAPDLQMPFEPSHENMANLKLYPDQPVEVLAADLRRAF
                     SGIVAGNVKEVGIRAIEEFGPYKINGDKEIMRRMDDLLQGFVAQHRMKLPGSAYIPCY
                     EICT"
BASE COUNT         5248 a         5688 c         5088 g         5305 t
ORIGIN      
        1 ttgcagccca accaactatt gtcccacaat tacagcaaca agttacgggt cttacgagta
       61 gcttaaactc acaagaaaag aaagaactga cgcacaagtt agaatctatt ttcaataata
      121 cccaagtgca aattgctgta ttaattgttt ccacaaccaa agatgagaca attgaacaat
      181 atgctacaag agtttttgac aattggcgtt taggagatgc caaacgtaat gatgggatac
      241 tgatcgttgt tgcctagtcg gatcgcactg tccgcatcca ggtaggttat gggctggaag
      301 aaaaggtaac cgatgctctg gctggagata tcatccgtag caacatgata cccgcattta
      361 aacaacaaaa attagctaag ggattagagt tagctataat cgctttgaat aaccaactca
      421 cttcacaaca ccaatatccg gctaatcctt cagaaagtga atcaacgtct tccactgatc
      481 attattactt tgctattttt tgggtatttg cagtgatgtt cttccctttc tggttttttc
      541 atcaaggcag taatttttgt cgcgcatgta aaagtggagt ctgtatttca gcaatccatc
      601 ttttagattt attcctgttc tccgataaaa ctttttccat tgctgtattt tccttctttt
      661 ttactttcac catatttatg gtctttacct gtttatgtgt acttcagaaa agagcatctg
      721 gtagaagtta tcattcagac aacagcggtt ccgctggagg ctcaagtggc ggcgcatccg
      781 gccgctggta actccaccat aaaaaatgcc agcccgaagg ctggcattat caaatcagat
      841 aaagtcagac ttatgcctgg cctttgatct ctttacgacc gttgtacggt gctttttcgc
      901 ccagagcttc ttcgatacga atcagctggt tgtatttagc aacacggtca gaacggctca
      961 tagaaccagt tttgatctgg cctgcagcag taccaacagc cagatcagcg atggtagcat
     1021 cttcagtttc accagaacgg tgagagataa ctgcagtgta gccagcatct ttcgccatct
     1081 tgattgcagc cagagtttcg gtcagagaac cgatctggtt gaatttgatc aggatggagt
     1141 tagcgatacc tttttcgata ccttctttca ggatcttggt gttggttacg aacaggtcgt
     1201 caccaaccag ctggattttg tcgcccagaa ctttggtctg gtatgcgaaa ccgtcccagt
     1261 cagattcgtc cagaccgtct tcgatagaaa cgatcgggta ctgtttggtc agttcttcca
     1321 ggaagtgagt gaattcttca gaggtgaacg ctttgttgcc ttcgccagcc agaacgtatt
     1381 taccatcttt gtagaattca gaagctgcgc agtccatcgc cagagtgatg tctttgccca
     1441 gttcataacc agcagcttta acagcttcag cgataacagc cagagcttca gcgttggaac
     1501 ccaggttcgg cgcatagcca ccttcgtcac caacagcagt gttcatgcct ttcgctttca
     1561 gaacttttgc caggtgatgg aaaacttcag aacccatgcg gatggcttct ttcacagttt
     1621 tcgcgccaac cggctgaatc atgaattcct ggatatcaac gttgttgtca gcgtgctcac
     1681 caccgttgat gatgttcatc atcggaaccg gcatggagta tttgcccgga gtgccgttca
     1741 gttcagcgat gtgctcgtac agcggcatac ctttagcagc tgcagcagct ttggcgttag
     1801 ccagagatac agccaggatt gcgttcgcgc cgaatttgga tttgttttcg gtgccgtcca
     1861 ggtcgatcat gatcttgtca atgccagcct gatctttagc atctttgcca atcagcgcct
     1921 gagcgatcgg gccgtttacc gcagcaacag ctttggttac gcctttaccc aggaaacggg
     1981 atttgtcgcc atcgcgcagt tccagagctt cacgggaacc agtagaagca cctgacggag
     2041 cagctgccat accgacgaaa ccaccctcca gatgtacttc ggcttcaaca gtcgggttac
     2101 cacgggagtc gatgatttca cgaccgatga tttttacgat tttggacatt aggttttcct
     2161 caagtcacta gttaaactga aactccagac aaacaacgcg tacccaaggt acgcgttgcc
     2221 gttctaactt ttttacttac ttcgcctgac gtttctggaa ctcgctggcg gctttcacaa
     2281 agcctgcaaa cagcgggtga ccatcacgtg gagtagaagt aaactccgga tggaactggc
     2341 aagccacgaa ccacgggtga ttcggaactt cgatgatctc gaccaactga tcatccccgg
     2401 aacggcccgc aacgcgcaga cctgcatctt caatctgttt caacagcatg ttgttgactt
     2461 cgtaacggtg acgatgacgc tcaacaattg tcggcgcatt gtatagctgg cgaaccaggc
     2521 tatcgtcaac caactggcac tgctgtgcgc cgagacgcat ggtaccgccg agatcgctct
     2581 tctcgctacg aacttcaacg ttgccgtttt catcgcgcca ctcggtaatc agcgccacaa
     2641 ccgggtactt acagtctggc acaaattctg tagagttggc gttctccatg ttggcaacat
     2701 ggcgagcgta atcaattaac gccacctgca tacccaggca aatgcccaga taaggaatat
     2761 tgttctcacg cgcaaaacgc gcggtcgtaa tcatgccttc tacgccacga tagccgaaac
     2821 cgccaggtac gaggattgcg tccagacctt taaggatttc aacgccgcgc gtttcaacat
     2881 cttgtgaatc gatcagtttg atgttgacgc tgacacgatt cttcagccca ccgtgtttca
     2941 gtgcttcgat cactgattta taagcatccg gcagttcaat gtacttgccg accataccga
     3001 tggtgacttc acttaccgga ttcgcttctt cgaagataac ctgttcccat tcggacagat
     3061 ttgcttccgg gcagtttaag ctgaatcgtt tacaaatata atcgtccagc ccctgagatt
     3121 tcaacaggcc cggaatttta tagatggaat cgacgtcttt cagagaaata accgcttttt
     3181 ccggaacatt acagaacaat gcaatcttcg cacgttcgtt cgccggtaca gcgcgatctg
     3241 aacgacaaat caggatgtca ggctggatac cgatggagag cagctctttt acagagtgct
     3301 gagtcggttt ggttttgact tcaccagacg ctgccatgta cggcaccagc gtcaggtgca
     3361 taaacagagt gtgctcacgg ccaatttcaa cagccatctg gcgaatagct tcgaggaacg
     3421 gcaaggattc gatatcacct actgtaccgc cgatttctac cagtactacg tcatgacctt
     3481 cgccaccttc cagcacgcgc tctttgattg cgttagtgat gtgcggaata acctgcacgg
     3541 ttgcgccgag gtagtcaccg cggcgttctt tacgcagaac gtcagagtag atacgacccg
     3601 tggtgaagtt gttgcggcgg ctcattttgg tacgaatgaa acgctcgtag tgccccaggt
     3661 ccaagtcggt ttcagcgccg tcttcagtaa cgaacacttc cccgtgttgg attgggctca
     3721 tagtacctgg atcgacgttg atgtaaggat ccagtttcat gatggtcaca ttgaggccac
     3781 gggcttcaag aatggctgcg agggaggctg cggcaatgcc tttacccaga gaggatacga
     3841 ccccgccggt cacaaaaata tagttcgttg tcatgctgaa cctgagaagt taggttgaaa
     3901 agacgatgga ataaccaaga cgggaaagca gtatacccga acatgacctg tgccacaaac
     3961 tttcattatc cctcctcttc gccagcgcac tattgaaatc aggagtgaga aaatagcccc
     4021 tttggggtaa atgtttttga cgcaaatcaa gcgcttgtca tttaaaaaat gacacaaatg
     4081 gcgcttgacc gcgtaattcc cttagagatc aatttcctgc cgttttacct gttgccagac
     4141 ttcttccatt gtttcgaggt caacacctgt catttccagt ccacgcgcgg caacaatacg
     4201 ctccacttcg cgaaaacgac gctcgaattt ttcgttcact ttttgcaatg cgatttctgc
     4261 tttcgtccct aaatggcgag ccagattaac cgtggcaaac agcaggtccc ccatttcctc
     4321 ctccagttta gcctggtcga caacagcctg ccgcgcctcg tacatcacct cgtcgatctc
     4381 ttcgtagact ttatcaacca ccgggccaag cgtcgtccag tcgaagccaa cgttggcgca
     4441 acgtttctgg attttttgcg cccgcattaa agccggtaaa ctacgaggaa tatcgtccag
     4501 cgccgaatgc tgcgctttct gcgcgcgctc ttcggttttg atttgctccc aacgggcaag
     4561 cacttcacta ctgttttcgg cagaactatc agcaaaaaca tgcggatggc gacgctctaa
     4621 tttatcgcta atagcagcgc aaatatcatt aaagtcaaag cgcccttctt cctgagccat
     4681 ttgcgcgtaa aacaccacct ggaatagcag atcgcccagt tcaccgcgaa gatcgtcaaa
     4741 atcttcacgg gcgatggcgt ccagcacttc gtaggtttct tcaagggtgt aaggcgcaat
     4801 ggtggcaaat gtctgctctt tatcccacgg gcagccgttt tccggatcgc gcaggcgctg
     4861 cataatagtg agcaaacggt cgatttgatt cattgaattg tcctgaaaat tgcgggtctg
     4921 tcaggtgtaa taccttcgag taaaagatat cgctggatac ggctgacgcc atatccagct
     4981 atgtaagact aactcccgtg caaccgacgc gcgtcgataa catccggcac ctggttgagt
     5041 ttacccagca cgcgccccag cacttgcagg ttgtaaatct caatggtcat gtcgatggtc
     5101 gccagttgct gtttggtgtc gctacggctg gcaacgccaa gcacgttcac cttctcgttg
     5161 gcgagaatgg tcgtgatatc acgtaacaac ccactacgat cattagccac cacgcggacc
     5221 accagcgaat atccggcgga gtagctctca ccccataccg cgtcaacaat gcgttctggc
     5281 gcatgggagc gcagttccgc cagttgttcg caatcggcgc ggtgtactga aataccgcgt
     5341 ccctgggtaa tgaagccgac aatctcatct ccaggaatcg gctggcagca gcgcgcgatg
     5401 tggtgcatca ggttaccaac accttcaacc accacacgac cgttatcttt actgcggttt
     5461 tgcggtgtgt agcttttttg ctgaagctgt ttcagcgcgg cggcgtcctg ctcttcggca
     5521 ctcggcttat taaattgcga ttgcaggaag ttcaccatct gattgagacg gatatccccg
     5581 ccaccaatcg ccgccagcaa ctcgtcgaca tcattgaagt tgtaacgcgg cagcagatgt
     5641 ttttctgctt ctttcaggct gatccccaga tgttccagct cgtcgtcaag gatttgccgc
     5701 ccagccagaa tgtttttgtc acggtcctgt ttacggaacc aggcgtgaat tttcgaacgc
     5761 ccacggctgg ttgtgacgta accgaggttt gggtttaacc agtcacggct ggggttcggc
     5821 tgtttctggg tgataatttc aatctggtcg cccatttgca gctggtaggt gaacggcaca
     5881 atgcgcccgc caatttttgc cccgatgcag cggtgtccga catcactgtg gatgtggtaa
     5941 gcgaagtcca gcggcgttga tcccgcaggc aaatcaacga catcaccttt cggcgtaaag
     6001 acatacaccc ggtcgtcaaa cacctgacta cgtacttcgt cgagcatttc gccggaatca
     6061 gccatctctt cctgccacgc aatcagttta cgcagccagg caatccggtc ttcatgtccc
     6121 gaacgtgcgc cgccagctgc cgcgccctct ttatatttcc agtgcgcagc aacacccaac
     6181 tccgcatctt catgcatctg tttggtgcgg atttggatct caacggtttt tccacccggc
     6241 cccagaacca cggtatgaat agactgataa ccgtttggtt tcgggttagc gacgtaatcg
     6301 tcaaactcat ccggcaggtg gcgatagtga gtgtgcacta tccccagtgc ggcataacaa
     6361 tcctgtaaac gctcggcgac aatacgtacc gcacgcacat caaacagctc atcgaaggcg
     6421 aggttctttt tctgcatttt gcgccagatg ctgtagatgt gtttcggacg accatacact
     6481 tcagctttaa cgccttcagc tttcatctca gcgcgcagat ggccgacaaa ctcttcgata
     6541 tagtgttcgc ggtcgagacg gcgttcatgc agcagttttg cgatgcgttt gtattcggtc
     6601 gggtggagat aacggaagca gtaatcttcc agctcccatt tcagttgccc aatcccaaga
     6661 cggttcgcca acggcgcgta gatattggtg cactcttttg ccgccagtac acgttcatct
     6721 tccggcgcat cttttacttc gcgcagatga gcaatacgct ccgccagttt gatgactacg
     6781 cagcgaaaat catcgaccat cgccaataac atccggcgaa cgttatcgac ctgttcggag
     6841 gaaacagaat cagtgtgcgt cgctttcagc tggcggatcg ccgccatatc acgcacgccg
     6901 tgaataaggt taacaaccga cttaccgacg ctctcacgca gcacatcttc gctgactacg
     6961 ttggcatcag ccagagggaa cagcagcgcc gcccgcagcg tgtcaatgtc catacttaat
     7021 gtcgagagga tctccaccat ctcaacacca cgccacaata acagactggc atccggatgc
     7081 ccctgcgtct gttgcagaca atacgcccag gtttcggcta agcactcaca cgacttctgg
     7141 ctggtaatac ccagacttgc gatccatttt tccggatcaa attcaccagc cttattgata
     7201 tgtgcacttc ttaccgcaac catcgtcctc tcctttaggg accagacctg ccgaaatcgg
     7261 caaatcgtaa ctatttaacg cgcgagaaaa gtaccatcga ttccagatgt cccgtgtgtg
     7321 ggaacatatc cagcatcgcc agtcgcgcaa tggtatatcc tgcttttaat aacgcttcgc
     7381 tatcccgagc cagcgttgca gggttacagg atacataaac tatacgaata ggttccagtt
     7441 ttataatttg ctgcataaca cctgcggcac ctgctcgcgc cgggtccagc aacactttat
     7501 cgaagccgtt tttcgcccac ggctgctttg tgacatcttc ttcaagattt tcgtgataaa
     7561 acgtcacatt ctgtaagcca ttaagacgcg cattctgctg gcctttttcc accagcgcag
     7621 gaacaccttc tacaccgacc acactggcag cttgtgtcgc caatggcagt gtaaagttgc
     7681 ccataccgca gaacagatcc agtacgcgat cttcaggttg cacatccagc cattccaacg
     7741 cacgcgctac cattttttgg ttcacacccg cattgacctg aataaaatcg cgcgggctaa
     7801 aagttaagcg caacccgttt gagtcatacc agggcatctc accagagacg gtttcgagta
     7861 tctcactatc gggggcgaga tacagatcca ggccttcaga atgcgaaaag cgttccagtt
     7921 tttcgcgatc tgccgaactt agcggtgcgg tatggcgcaa aatcatcagc gtgccgctgg
     7981 ttgcctgtac cagttcaaca tgaccaagat ggcgcatagc ttgtaagctg cccagacatg
     8041 ccctgacttt gggcagcaat gcttcaagtt ggggcgctaa aatggggcat tgtttgacgt
     8101 cgacaatgtc actggagccc gctttgcgaa accccatctg aagttgctgt gttttcggta
     8161 agtagttcag acttaaacgc gcgcggcggc gatagcccca gggaacatcg gcgatcactt
     8221 cagagacatc gtgtttcatt aatcgggcga gtgccgcact tttgcttcgc tgctgtaaat
     8281 ccacgctggc gtgttgttgc tgacagccgc cgcatacgcc aaaatgagga cagcgtggcg
     8341 tttcgcgttc cgggctatcg cttaaccggc gtacgacttt agcgcgggca tactgttttt
     8401 tatcttcagt aacagcaact tccgcgtttt cctgcggcaa taatccgggg ataaataacg
     8461 ttttgccgtt atgtcgcgcc acgccctgac caaaagagtc gaggtcgttg actgaaacgg
     8521 ttatgatctg acgcgtcgtc gtgcgtcgtt ttgcagagta gaattgcgcc attggcgaga
     8581 ctttctcaat ttaacagtgt gaccttaatt gtcccataac ggaactccat gaccaactac
     8641 agcctgcgcg cacgcatgat gattctgatc ctggcaccga ccgtccttat tggtttattg
     8701 ctgagtatct ttttcgtcgt gcatcgctat aacgacttgc agcgtcaact ggaagatgcc
     8761 ggtgctagca ttattgagcc gcttgcagtt tctactgaat atggcatgag cctgcaaaat
     8821 cgcgaatcta tcggtcagtt aataagcgta ctgcatcgtc gccattccga tattgttcgc
     8881 gcgatttcgg tttatgatga aaataaccga ctctttgtca cctccaattt tcatcttgat
     8941 ccctcatcaa tgcagctcgg cagcaacgtg ccgtttcctc gccagctcac tgtcactcgt
     9001 gacggcgata ttatgatcct ccgcacgccg attatttctg agagttactc ccccgacgaa
     9061 tcgcccagta gcgatgccaa aaatagtcaa aatatgttgg gatatattgc gcttgagctg
     9121 gatcttaaat cggttcgctt gcagcaatat aaagagatct ttatttcctg cgtgatgatg
     9181 ctgttttgta tcggtattgc gcttattttt ggctggcgct taatgcgcga tgtaaccggt
     9241 ccgattcgca acatggtgaa taccgtcgac cgcatccgtc gcgggcaact cgacagccgg
     9301 gtggaaggat ttatgctcgg cgagctggat atgctgaaaa acggtatcaa ctcgatggca
     9361 atgtcgctgg ctgcttatca cgaagagatg cagcacaata tcgaccaggc gacgtccgat
     9421 ctgcgtgaaa cgctggagca gatggaaatt cagaacgttg agttggatct ggcgaaaaag
     9481 cgcgcccagg aagcggcgcg tattaaatcc gagtttctgg caaatatgtc acacgagctg
     9541 cgtacaccac tgaatggtgt tattggcttt acccgcctga cgctgaaaac agaattaaca
     9601 ccaacgcagc gcgatcacct gaatacgatt gaacgttcgg caaataattt gctggcaatt
     9661 attaatgatg ttctcgactt ctcgaaactg gaagcaggta agctgattct ggaaagtatt
     9721 ccattcccac tacgcagcac gctggatgaa gtcgttactc tgctggcaca ttcttctcac
     9781 gataaagggt tggaactgac gctcaatatt aaaagcgacg tgcctgataa cgtgatcggc
     9841 gacccactgc gattacagca aatcatcact aacctggtgg ggaatgcaat taaattcacc
     9901 gagaatggca acattgatat tctggtagaa aaacgtgcgc tgagtaatac caaagtgcag
     9961 attgaagtgc agattcggga taccggcatt ggtattcctg aacgcgatca atcgcgctta
    10021 ttccaggcct tccgacaggc tgatgccagt atttcccgcc gtcatggtgg caccgggctg
    10081 gggctggtga ttacacaaaa actggttaat gaaatgggcg gcgatatttc gttccatagc
    10141 cagccgaatc gcggttcaac tttctggttc cacattaatc tcgatctgaa cccgaacatt
    10201 attatcgaag ggccatccat ccagtgcctc gcaggtaagc gcctggccta tgtcgaacca
    10261 aactccgcag cagcgcaatg cacgctggat attttaagtg aaacgccgct ggaagtggtt
    10321 tatagcccaa cgttctccgc gctgcctccc gcgcattacg acatgatgtt gttaggcatc
    10381 gcggtgacct tccgcgagcc gctaacaatg caacatgagc gattagcgaa agcggtatcg
    10441 atgaccgatt tcctgatgct ggcacttcct tgccatgcac aagttaatgc tgaaaaactc
    10501 aagcaagatg gtatcggcgc gtgtctgctg aaaccattaa cacctacgcg cctgttgcct
    10561 gccctgacgg aattttgtca tcacaaacaa aacacgcttt tgcctgtaac cgatgaaagt
    10621 aagctggcaa tgacagtcat ggcggttgat gacaaccccg ctaacctgaa acttatcggc
    10681 gcattgctgg aagatatggt gcaacatgtg gaactttgcg atagcgggca tcaggcagtt
    10741 gaacgggcga aacagatgcc gttcgatttg atcttaatgg atattcaaat gccggacatg
    10801 gatggcattc gggcctgcga actcatccac caactcccgc atcagcgaca aacgccggtt
    10861 atcgcggtaa cggcgcatgc aatggccggg caaaaagaga agctgcttgg cgcagggatg
    10921 agcgattatc tggcgaaacc gattgaagaa gagcgattgc ataatttgtt gttgcgctac
    10981 aagcctggca gcggtatttc gtctcgcgtc gtgacgcccg aagtcaacga aattgtggtg
    11041 aacccgaatg cgaccctcga ctggcaactg gcactacgcc aggcagcagg aaaaaccgat
    11101 ttagcgcgcg atatgctgca aatgttactc gatttcctgc ctgaagttcg caacaaagtt
    11161 gaggaacagc tggctggaga aaacccggaa ggcctggttg atttgattca taaactgcat
    11221 ggcagttgcg gctatagcgg cgtaccacgt atgaagaatc tctgccagct gatcgaacaa
    11281 cagctacgta gcggtactaa agaagaagat ttggagccgg agctgctgga actgttggac
    11341 gagatggata atgtcgcgcg cgaagccagc aaaattctcg ggtaacggac gtcttgtcga
    11401 gtagcaatcg gctttttatg acgctggttt cagattggtg acaaagtgcc tgtgtttatg
    11461 ccggatgcgg cgttaacgcc ttatccggcc tacaaaacca attaaattca atgaattaaa
    11521 aaatgatgta gacctgatac atagcacaac aggctatttt gcgtttagca tcagtctcaa
    11581 accggctcca gatagagccg gttttggttt tctgtcttaa cgcaccatgc acgggcgctt
    11641 gttatcgaac gtccagccag gaatcagata ctgcattccc atcgcatcgt cacgcgcgcc
    11701 aagcccgtgt ttctgataca gctcatgggc tttcatcact tgatccatat cgatttctac
    11761 acccagcccc ggtttttctg gcacctgtac cagcccgcct ttgatctcaa acggttcttt
    11821 ggtcaggcgc tgattacctt cctgccagat ccagtgcgta tcaatagcag taattttacc
    11881 cggtgcagcg gcggcaacat gggtaaacat cgccagggaa atatcgaagt ggttgtttga
    11941 gtgtgaaccc caggtcaggc caaattcatg gcacatttgc gccacacgta ccgaaccttg
    12001 catagtccag aaatgcggat ccgccagcgg gatatcaacg gattgtaacg agagcgtatg
    12061 gcccatctgc cgccagtcgg tggcgatcat attggttgca gtcggcaggc cagtcgcgcg
    12121 acggaactct gccatcactt cacgcccgga gaaaccttgc tccgcaccac acggatcttc
    12181 tgcataagcc agcgaacctt tcaggtattt accaatttta attgcttcgt tcagcgacca
    12241 ggcaccgtta ggatcgagcg taatacgcgc ctgcgggaag cgttgcgcca gtgccacaat
    12301 agactcggct tcttcttccc cggccagtac accgcctttc agtttgaaat cgttgaagcc
    12361 atatttttca tacgccgctt ccgccaggcg caccaccgca tccggcgtca tcgcttcttc
    12421 atgacgcagg cgataccagt cgcatgagtc atccggctgg ctttgatacg gcagaggtgt
    12481 ggctttgcga ttaccgacga agaacagata accgagcatt tcgacttcgc tacgctgttg
    12541 accatcgccc agcagcgatg ccacgtttac ccccagatgc tgccccagca gatccagcat
    12601 tgccgcttct atcccggtaa ctacatgaat agtggtacgc aaatcaaatg tctgcaaacc
    12661 gcgcccacta gcatcacgat cggcaaaagt attacgcacc agcgtcagaa cgtttttgta
    12721 ttcacccagc gttttaccta ccaccagcgg aatcgcatct tccagcgttt tacggatttt
    12781 ctcgccgccg ggaatttccc ctacgccagt gtgaccggaa ttatctttga taatcacaat
    12841 attacgcgtg aagaacggtg cgtgtgcacc actcagattc atcagcatac tgtcatgacc
    12901 cgccaccggg atgacctgca tttcagtaac aacaggcgtc gtaaattgag aactcatatt
    12961 tatgtccttt ttcagaatca atgacggccg aaaacgggac gtttacggtc aaaggtccag
    13021 ccggggatca ggtactgcat cggacctgcg tcgttacgcg caccgcaagg cagacgttta
    13081 taggcctcat gtgccttttg tacctgttcc cagtccagtt ccacgcccag accgggcgca
    13141 tcaggaacgg caatttttcc gtttttaatc tccagcggat ttttggtcag gcgacaatcg
    13201 ccctcctgcc aaatccagtg ggtatcgata gcggtaggat tacccggtgc cgccgcgccc
    13261 acatgggtaa atatcgccag agagatatcg aaatggttgt tggaatggca gccccaggtc
    13321 agcccccagt cgtcgcaaag ctgcgccacg cggactgcac cggaaagcgt ccagaagtgc
    13381 ggatcggcaa gtggaatatc taccgcattg agcatcaccg catgacccat ttcgcgccag
    13441 ttggtggcga tcatgttagt cgcgacgggc aagccggtcg cccgtcgaaa ctccgccatc
    13501 acttcacgtc cggagaagcc ctgttctgcg ccgcatggat cttcggcata ggtaagaaca
    13561 tcattcagcc ctttgcataa agaaatggct tcatcaagca gccatgcacc gttgggatca
    13621 acggtaatcc gcgcatccgg gaagcttttc ttcaatgcac gaacagtgtc gatttcttgc
    13681 tcgccaggta acaccccgcc cttaagtttg aaatctttaa agccatagcg atcctgtgag
    13741 gcttccgcca gacgcacaac ggcttcgctg ttcatcgctt tctgatggcg caactgatac
    13801 cactcatggt tgcccggcgt attttccaga taaggaagat cggttttggt ccgatcacct
    13861 atataaaaca gataaccgag gacggtaata gtctcgcgtt gcttgcctgg ccccaacagt
    13921 tcgcagaccg gaacattcag cgccttacct agcaggtcaa gcaaggcggc ttccagcgcc
    13981 gccacggcat taacgcgcaa ttcaaaagtc caggcacctt tgccaaaggt atcaaaatcg
    14041 gctgcctgat tacctttatg tacctgctga accactttat tcaggcgcgc aacttcctgg
    14101 cccagaacca tcggaatagc atcgaccagt gtttgataaa tcacctctcc gcccggcgct
    14161 tcaccaatgc cggtatgccc ggcgttatcg gtgagtacca cgatattgcg ggtgaagtat
    14221 gcgttatgcg caccaccaat attgagcaac atgctgtcat gtccggcgac cggaatgact
    14281 ttcatatcag taataacagg actggattgt gtcgccatct tattgccccg cgacaggttt
    14341 cagctcgata cgcttgatat cgcccaccag caccaggtag ctcagtaccg cgattagggc
    14401 atgaacacca acataaatca gcgccccatt aaacgagcca gtcgtgccaa cgatataacc
    14461 aattgcgatt ggcgtgacga tgccagaaat gttaccgaac atgttgaaca ggccaccgga
    14521 aagaccgctg atctcttttg gcgcggtatc tgccattact gcccaaccca gcgccccgat
    14581 gcccttaccg aagaaggcca gcgccataaa gccgatgatc atccactcaa cgttgacgta
    14641 gttgcagaac accatcacca tcgacagcaa catgcccatt acgatcggtg ttttacgcgc
    14701 aatgttcagc gatcccgtgc ggcgcatcag ccagtcggaa ataattccac ccagcacacc
    14761 gccgataaaa ccgcaaaccg ccggaacgga agccacaaag cccgctttca gaatcgacat
    14821 cccacgcgcc tgcaccagat aaaccggaaa ccaggtaatg aaaaagtaag tcagggcgtt
    14881 gatacagtac tgaccgatat aaacgccgat catcatccgt gacccgagca gctgtttgat
    14941 ctgcccccac ttcacgctga acggcacttt aactttggtg ctttgctgat ccatattgat
    15001 cagcgcgcca cccgcggcga tatactccag ctctttctga tttacccccg gatgttgatt
    15061 tggctcgtgg atgactttca accagataaa gctgatgaca atgcccagac cgcccataaa
    15121 gaagaagacg tgtgaccagc ccacttcatg cgtcagccag cccataatcg gcgcgaagat
    15181 caccgttgcg aagtattgag cggagttaaa aatcgacacc gccgttcccc tttcctgcac
    15241 cggaaaccag gccgcaacaa tgcgactgtt gccggggaaa gatggcgctt cagcaagccc
    15301 gaccaggaag cgcagagtaa acagggcaac gataatgccg aatccactaa agatatcgac
    15361 gaagccttgc agcaaggtaa acatcgacca gataaagatc gaccagaagt agacgcgttt
    15421 tgaaccaaaa cgatccagca accaaccacc agggatctgc ccgataacat aagcccatga
    15481 gaaggcagag aacacatagc ccattcccac gggatcaagg ccgatatctt tggccatttc
    15541 cgaaccggca atagaaagcg tagcgcggtc gccgtagttg aaggatgtga cgataaacaa
    15601 catcaccact atccagtaac gagcattcgt gcgtttttcc acactgctcg cagcctgact
    15661 taaagaactc attgttgcac tcctgaaaat tcgcgttagc cacgctcact ctggactgcg
    15721 acatcgccag gaaatcagag gtgacgtagg gtgttttttg ccgtttttat aggtcgttcg
    15781 ccgaatacgg cgcgtgttta tatctggcaa tagcagtata aaaagcgcgc catagcggct
    15841 caccgtgcaa caacacaaca ttaatgcgtt caatgaggcc cgattttggc attagccctg
    15901 gacggtggaa tccacttcac ggaaatgaaa acaagaacaa gaaaggaagg gtaaaaacga
    15961 agaaataaag aagagtatga aatggatcgc ttgactctag gcaaacgcca gtaaaaatcc
    16021 gcgctatgaa gcagttttta ctggcatttg cctgaaaaga ttcgattcag caccgctaaa
    16081 acgacattta ccgctcgctg acatatcagg acaacagcgt gccccactgt tcgacccacg
    16141 gatttgattc cgtttccggt tccgggttct cactggcgtc aatcaacagc atttcgccaa
    16201 cccgctgagc gctctgttcc tgcaacaagg catcgaactg tttgccgcca ttgcaaaaat
    16261 ttacataact gctgtcgccg agcgcaatca cgccataacg cagattcggc tggaagccca
    16321 gactatcttt gattccctga aagagcggca caatgctatc aggaaggtcg ccctgcccgg
    16381 tcgtggacgt aaccaccaga acatacttat cctgataggg cagccagtcg cttaattcag
    16441 gatcttcaaa taccgttgct ttgtggccct gtgcggtcag aattgcttct gcttcttccg
    16501 ccactaacag cgaatttccg tacatggtgc cgacaaaaat accaatttcc gccataccgt
    16561 tttctccctg gattaggaac ttatctctcc atcctgaccc gatggcgcac tgaactcaac
    16621 cctttcattt tcaggaagca gaccgcgcca gccaaattgt gataacgcct gcatccaggt
    16681 gtcgtccagg cccgcgtgaa tagtcagcgg ctcgccagta aaaggatgcg tcaatgacaa
    16741 ctgactggca tgtagcatta accgctggag gccaaaatgc tcagcaccgc tgcgattctg
    16801 gcgtaaatcg ccatgtttgc tatcgccaat aatcggatga cgcaaatggg caagatgtcg
    16861 tcgaagctga tgtttgcgtc cggttttcgg ctccagttcc accaggccgt agcgcgtggt
    16921 cgggtaacgt ccggtcgcta ccggcatttc tacggtcgcc agaccgcgat aatgcgtcac
    16981 tgctggctgc gggcctttat cttcgcgggc aaatttatca gcgattttgt ccagttcttc
    17041 caccagtgga taatccaaca ccgcttcttc catcaaccag ccgcgcacaa tcgcatggta
    17101 acgtttctgg atttggtgct gttcaaactg ttgtgccagc agccgtccgg cctcgctgga
    17161 taatcccatc aacaacacac cagaagtggg tcggtccaga cgatgagcag taaaaacatg
    17221 ctgtcctatc tggtcacgca cggtttgcat gaccactact ttctcgtcgc gatccagcca
    17281 gctacggtga accagccagc cggagggttt atttaccgca accagccatt catcctgata
    17341 gagtatttcc agcattagct cgcatcatcc gcaaaaagag catccagttt ttccaactca
    17401 gccagaataa gcgcgcgttg cggatggtcc gtcgccagcg ccatttcata atagggtgca
    17461 acagcaaaag cgcccggtaa cggctggttg ttatttaaca aatcgtgcat tcgcgggatc
    17521 agcccccact gcaaccactc cagtggttcc atggtgtcca taaagaacgg ttgggtacta
    17581 ttaaattgat gcggctgggg ttcatcgttt cgccagtgct gatgttcacg cagtaacgct
    17641 tcaagcgcct gcaactggag acgaacgcgg tcatgagtgg tcataaagca acctcaataa
    17701 gaaaaactgc cgcgaaggat agcaggaata aaaaaaggga gcactgtatt cacagtgctc
    17761 ccggttcgtt tcgcagcatt ccagctactt ttgttgctcc ctgctcatcc ttgacaactt
    17821 ttcctctggc cttgcggcca atcgttcatc ctgaactatt gcttcctgct cacaccaccc
    17881 cgatgtgata cttcatcctg aagtgtccct ggccttcctg acccaccgaa tcatcctgac
    17941 cggttctcat tctccatcct ggaggtgtcc tttaacgcgt cctgcgtcat cctcttcgct
    18001 tcatccagaa gcctttccct gaaacaccat cctggtgtgt cctgcagaag tgtcatcatc
    18061 ctgatgttca cttcgttgtc tgactccctg tcgacgaaga tagaatcgtc tttttcgcga
    18121 agtcttacaa ggcgcttaca gacaatgcct taaggaaaat ttcatatgaa agttaagaat
    18181 taacaatcaa cattttgatt ttaaatgaaa ataacgattc ttatcttttt aagtattcgc
    18241 ttatttcctg gcgatctcct acagagtctg taagagatct ctcacaacca gatggggtat
    18301 ttggattaca gaagaggctt aagttgatta aggaattccg caagatttga ggctaaattc
    18361 gtccgtttac gtgttcccag cgtctctttg cacacttcac cggagagatt acacaccgag
    18421 atgacctcta actcttcttc cagcgtcgcg ataaatagcg tgggcggcag ttttagcctt
    18481 ttctgagtca ccagatgacc aatcagattt tcctgcaccc gacggaagtc gtcttcactc
    18541 caggtttgca gcaatgtcag tttgatatcg ccaaactgcg cgtgcatatc cccggcaaac
    18601 tgagtcgtat aaaaggtatg aattgtgggt tgtatcacaa tatcaaaagc gcgttcaacc
    18661 gcgtttacat tttgttcccc ggtaaacggc tggggttgcc agtatacggc atcttcggta
    18721 gttgaaataa tgcacggtga aggaacaccg tacaattcct cacttaacgg ccagctttta
    18781 tgctcttcat gccacgcatc gcagtagcgt gcagtaaaat ctttcagggc ttgtgcggtc
    18841 aaatcgtcca ctaatttctc tcttcactta aaccagatac acttgtcgtt tagtttatct
    18901 ggtttatgac ggtgaaacat gtcttcttat gcaaaccatc aggcacttgc gggcctgact
    18961 cttggaaaat caaccgatta ccgggatacc tatgacgcca gcctactgca aggcgttcca
    19021 cgcagcctga atcgcgaccc gctgggtctg aaagcggata acctgccttt tcacggtacg
    19081 gatatctgga cgctgtatga actttcctgg ctgaatgcga aaggtttgcc gcaggtcgct
    19141 gtcggtcatg ttgaacttga ttacaccagc gtaaatctga ttgagtcgaa gagttttaag
    19201 ctctatctca acagttttaa ccagacgcgt tttaataact gggatgaggt gcgccagacg
    19261 ctggagcgcg acttaagcac ttgcgctcag ggtaagatta gcgtggcgtt atatcgtctt
    19321 gatgaactgg aaggccagcc gataggtcat ttcaatggca cttgcattga tgacaaggat
    19381 atcactatcg ataactatga attcactact gactatctgg agaatgccac cagcggtgaa
    19441 aaagtagtgg aagagacgct cgtcagccac ctgctgaaat caaactgcct gatcacccat
    19501 caaccagatt ggggttcgat ccaaattcag tatcgtggac gccaaattga cagagaaaaa
    19561 ctgctgcgtt acctggtctc attccgtcat cacaacgagt tccacgaaca gtgcgtggaa
    19621 cgcatcttta atgacctgtt acgcttctgc cagccagaga aattgagcgt ttacgcacgt
    19681 tatacccgtc gtggcggtct ggacattaac ccgtggcgca gtaatagcga ttttgtccca
    19741 tcgaccacaa gactggttcg gcaataaatt ttttctcaat tttgcgtgct ggattcacgc
    19801 agaaggttgt gaaaggtcat caggcagggc tattgtaatc aaagggaatg acgatattcg
    19861 tcccataagg agtttttctt gattacacat attagcccgc ttggctccat ggatatgttg
    19921 tcgcagctgg aagtggatat gcttaaacgc accgccagca gcgacctcta tcaactgttt
    19981 cgcaactgtt cacttgccgt actgaactcc ggtagtttga ccgataacag caaagaattg
    20041 ctgtctcgtt ttgaaaattt cgatattaac gtcctgcgcc gtgaacgcgg cgtaaagctg
    20101 gaactgatta atcccccgga agaggctttt gtcgatgggc gaattattcg cgctttgcag
    20161 gccaacttgt tcgcggttct gcgagacatt ctcttcgttt acgggcaaat ccatcatacc
    20221 gttcgttttc ccaacctgaa tctcgacaac tccgtccaca tcactaacct ggtcttttcc
    20281 atcttgcgta acgctcgcgc gctgcatgtg ggtgaagcgc caaatatggt ggtctgctgg
    20341 ggcggtcact caattaacga aaacgagtat ttgtatgccc gtcgcgtcgg gaaccagctg
    20401 ggcctgcgtg agctgaatat ctgcaccggc tgtggtccag gagcgatgga agcgccgatg
    20461 aaaggtgctg cggtcggaca cgcgcagcag cgttacaaag acagtcgttt tattggtatg
    20521 acagagccgt cgattatcgc cgctgaaccg cctaacccac tggtcaacga attgatcatc
    20581 atgccggata tcgaaaaacg tctggaagcg tttgtccgta tcgctcacgg catcattatc
    20641 ttccctggcg gtgtgggtac ggcagaagag ttgctgtatt tgctgggaat tttaatgaac
    20701 ccggccaaca aagatcaggt tttaccattg atcctcaccg gcccgaaaga gagcgccgac
    20761 tacttccgcg tactggacga gtttgtcgta catacgctgg gcgaaaacgc gcgccgccat
    20821 taccgcatca tcattgatga cgccgctgaa gtcgctcgtc agatgaaaaa atcgatgccg
    20881 ctggtgaaag aaaatcgccg tgatacaggc gatgcctaca gctttaactg gtcaatgcgc
    20941 attgcgccag atttgcaaat gccgtttgag ccgtctcacg agaatatggc taatctgaag
    21001 ctttacccgg atcaacctgt tgaagtgctg gctgccgacc tgcgccgtgc gttctccggt
    21061 attgtggcgg gtaacgtaaa agaagtcggt attcgcgcca ttgaagagtt tggtccttac
    21121 aaaatcaacg gcgataaaga gattatgcgt cgtatggacg acctgctaca gggttttgtt
    21181 gcccagcatc gtatgaagtt gccaggctca gcctacatcc cttgctacga aatctgcacg
    21241 taatctcccc tcccccggtg taatgccgat cagttaagga tcagttgacc gatccagtgg
    21301 ctgtgtaaga atccggaaac gctcacttg
//
LOCUS       DAJTRC010000065        20807 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.065, whole genome shotgun sequence.
ACCESSION   DAJTRC010000065 DAJTRC010000000
VERSION     DAJTRC010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20807)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20807)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20807
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.065"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..696
                     /locus_tag="OO357_002627"
     CDS             <1..696
                     /locus_tag="OO357_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS150 family transposase"
                     /protein_id="HCR5887532.1"
                     /translation="ENRGRYGYRRVTLSLHREGKQINHKAVQRLMGTLSLKAAIKVKR
                     YRSYRGEVGQTAPNVLQRDFKATRPNEKWVTDVTEFAVNGRKLYLSPVIDLFNNEVIS
                     YSLSERPVMNMVENMLDQAFKKLNPHEHPVLHSDQGWQYRMRRYQNILKEHGIKQSMS
                     RKGNCLDNAVVECFFGTLKSECFYLDEFSNISELKDAVTEYIEYYNSRRISLKLKGLT
                     PIEYRNQTYMPRV"
     gene            798..950
                     /gene="hokA"
                     /locus_tag="OO357_002628"
     CDS             798..950
                     /gene="hokA"
                     /locus_tag="OO357_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001135724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokA"
                     /protein_id="HCR5887533.1"
                     /translation="MPQKYRLLSLIVICFTLLFFTWMIRDSLCELHIKQESYELAAFL
                     AYKLKE"
     gene            complement(1137..1349)
                     /gene="cspA"
                     /locus_tag="OO357_002629"
     CDS             complement(1137..1349)
                     /gene="cspA"
                     /locus_tag="OO357_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone/antiterminator CspA"
                     /protein_id="HCR5887534.1"
                     /translation="MSGKMTGIVKWFNADKGFGFITPDDGSKDVFVHFSAIQNDGYKS
                     LDEGQKVSFTIESGAKGPAAGNVTSL"
     gene            complement(1630..1920)
                     /locus_tag="OO357_002630"
                     /pseudo
     CDS             complement(1630..1920)
                     /locus_tag="OO357_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312467.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator"
     gene            2354..3064
                     /locus_tag="OO357_002631"
     CDS             2354..3064
                     /locus_tag="OO357_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3053 domain-containing protein"
                     /protein_id="HCR5887535.1"
                     /translation="MATGKSCSRWFAPLAALLMVVSLSGCFDKEGDQRKAFIDFLQNT
                     VMRSGERLPTLTADQKKQFGPFVSDYAILYGYSQQVNQAMDSGLRPVVDSVNAIRVPQ
                     DYVTQSGPLREMNGSLGVLAQQLQNAKLQADAAHSALKQSDDLKPVFDQAFTKVVTTP
                     ADALQPLIPAAQTFTQQLVMVGDYIAQQGTQVSFVANGIQFPTSQQASEYNKLIAPLP
                     AQHQAFNQAWTTAVTATQ"
     gene            complement(3114..4088)
                     /gene="ghrB"
                     /locus_tag="OO357_002632"
     CDS             complement(3114..4088)
                     /gene="ghrB"
                     /locus_tag="OO357_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrB"
                     /protein_id="HCR5887536.1"
                     /translation="MKPSVILYKALPDDLLQRLQAHFTVHQVANLSPQTVVQNAAIFA
                     EAEGLLGSNENVDAALLEKMPKLRATSTISVGYDNFDVDALTARKILLMHTPTVLTET
                     VADTLMALVLSTARRVVEVAERVKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMAL
                     AQRVHFGFNMPILYNARRHHKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLF
                     GAEQFAKMKSSAIFINAGRGPVVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLS
                     MANVVAVPHIGSATHETRYGMAACAVDNLIDALQGKVEKNCVNPHVAD"
     gene            complement(4192..4842)
                     /locus_tag="OO357_002633"
     CDS             complement(4192..4842)
                     /locus_tag="OO357_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family lipoprotein"
                     /protein_id="HCR5887537.1"
                     /translation="MFILLAAVVSGALAVSGCTTNPYTGEREAGKSAIGAGLGSLVGA
                     GIGALSSSKKDRGKGALIGAAAGAALGGGVGYYMDVQEAKLRDKMRGTGVSVTRSGDN
                     IILNMPNNVTFDSSSAPLKPAGANTLTGVAMVLKEYPKTAVNVIGYTDSTGGHDLNMR
                     LSQQRADSVASALITQGVDASRIRTQGLGPANPIASNSTAEGKAQNRRVEITLSPL"
     gene            5005..7203
                     /locus_tag="OO357_002634"
     CDS             5005..7203
                     /locus_tag="OO357_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418007.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HCR5887538.1"
                     /translation="MANSSSRYSVLTAAHWGPMLVETDGETVFSSRGALATGMENSLQ
                     SAVRDQVHSNTRVRFPMVRKGFLASPENPQGIRGQDEFVRVSWDEALAGGYTGHLGDY
                     STGAAQAIMPYVVGGSEVYQQQTSWPLVLEHSDVVVLWSANPLNTLKIAWNASDEQGL
                     SYFSALRDSGKKLICIDPMRSETVDFFGDKMEWVAPHMGTDVALMLGIAHTLVENGWH
                     DEAFLARCTTGYAVFASYLLGESDGIAKNAEWAAEICGVGAAKIRELAAIFHQNTTML
                     MAGWGMQRQQFGEQKHWMIVTLAAMLGQIGTPGGGFGLSYHFANGGDPTRRSAVLSSM
                     QGSLPGGCDAVDKIPVARIVEALENPGGAYQHNGMNRHFPDIRFIWWAGGANFTHHQD
                     TNRLIRAWQKPELVVISECFWTAAAKHADIVLPATTSFERNDLTMTGDYSNQHLVPMK
                     QVVPPRYEARNDFDVFAELSERWEKGGYARFTEGKSQLQWLETFYNVARQRGASQQVE
                     LPPFAEFWQANQLIEMPENPDSERFIRFADFCRDPLAHPLKTASGKIEIFSQRIADYG
                     YPDCPGHPMWLEPDEWQGNAEPEQLQVLSAHPAHRLHSQLNYSSLRELYAVANREPVT
                     IHPDDAQARGITEGDMVRVWNSRGQILAGAVISEGIKPGVICIHEGAWPDLDLTADGI
                     CKNGAVNVLTKDLPSSRLGNGCAGNTTLAWLEKYNGPELTLTAFEPPASS"
     gene            complement(7172..7612)
                     /locus_tag="OO357_002635"
     CDS             complement(7172..7612)
                     /locus_tag="OO357_002635"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HCR5887539.1"
                     /translation="MIREAQRSELPAILELWLESTTWGHPFIKANYWRDCIPLVRDAY
                     LANAQNWVWEEDGKLLGFVSIMEGRFLAAMFVAPKAVRRGIGKALMQYVQQRYPHLML
                     EVYQKNQPAIDFYRAQGFHIVDCAWQDETQLPTWIMSWPVVQTL"
     gene            complement(7609..8172)
                     /gene="tag"
                     /locus_tag="OO357_002636"
     CDS             complement(7609..8172)
                     /gene="tag"
                     /locus_tag="OO357_002636"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HCR5887540.1"
                     /translation="MERCGWVSQGPLYIAYHDNEWGVPETDSKKLFEMICFEGQQAGL
                     SWITVLKKRENYRAYFHQFDPVKVAAMQEEDVERLVQDAGIIRHRGKIQAIIGNARAY
                     LQMEQNGEPFPDFVWSFVNHQPQVTQATTLSEIPTSTSASDALSKALKKRGFKFVGTT
                     ICYSFMQACGLVNDHVVGCCCYLGNKP"
     gene            8330..9028
                     /locus_tag="OO357_002637"
     CDS             8330..9028
                     /locus_tag="OO357_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5887541.1"
                     /translation="MIIKKSGGRWQLSLLASVVISAFFLNTAYAWQQEYIVDTQPGHS
                     TERYTWDSDHQPDYNDILSQRIQSSQRALGLEVNLAEETPVDVTSSMSMGWNFPLYEQ
                     VTTGPVAALHYDGTTTSMYNEFGDSTTTLTDPLWHASVSSLGWRVDSRLGDLRPWAQI
                     SYNQQFGENIWKAQSGLSRMTATNQNGNWLDVTVGADMLLNQNIAAYAALTQAENTTN
                     NSDYLYTMGVSARF"
     gene            9257..10465
                     /locus_tag="OO357_002638"
     CDS             9257..10465
                     /locus_tag="OO357_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5887542.1"
                     /translation="MTPSNYQRTRWLTLIGTIITQFALGSVYTRSLFNGALSAKLDAP
                     VSQVAFSFGLLSLGLAISSSVAGKLQERFGVKRVTVASGILLGLGFFLTAHSNNLMML
                     WLSAGVLVGLADGAGYLLTLSNCVKWFPERKGLISAFAIGSYGLGSLGFKFIDTQLLE
                     TVGLEKTFVIWGAIALVMIVFGATLMKDAPKQEVKTSNGVVEKDYTLAESMRKPQYWM
                     LAVMFLTACMSGLYVIGVAKDIAQSLAHLDVVSAANAVTVISIANLSGRLVLGILSDK
                     IARIRVITIGQVISLVGMAALLFAPLNAVTFFAAIACVAFNFGGTITVFPSLVSEFFG
                     LNNLAKNYGVIYLGFGIGSIFGSIIASLFGGFYVTFYVIFALLILSLALSTTIRQPEQ
                     KMLREAHGSL"
     gene            10789..12480
                     /gene="eptB"
                     /locus_tag="OO357_002639"
     CDS             10789..12480
                     /gene="eptB"
                     /locus_tag="OO357_002639"
                     /EC_number="2.7.8.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kdo(2)-lipid A phosphoethanolamine
                     7''-transferase"
                     /protein_id="HCR5887543.1"
                     /translation="MRYIKSITQQKLSFLLAIYIGLFMNGAVFYRRFGSYAHDFTVWK
                     GVSAVVELAATVLVTFFLLRLLSLFGRRSWRILASLVVLFSAGASYYMTFLNVVIGYG
                     IIASVMTTDIDLSKEVVGLNFILWLIAVSALPLILIWNNRCRYTLLRQLRTPGQRIRS
                     LVVVVLAGIMVWAPIRLLDIQQKKVERATGVDLPSYGGVVANSYLPSNWLSALGLYAW
                     ARVDESSDNNSLLNPAKKFTYQAPQNVDDTYVVFIIGETTRWDHMGIFGYERNTTPKL
                     AQEKNLAAFRGYSCDTATKLSLRCMFVRQGGAEDNPQRTLKEQNIFAVLKQLGFSSDL
                     YAMQSEMWFYSNTMADNIAYREQIGAEPRNRGKPVDDMLLVDEMQQSLGRNPDGKHLI
                     ILHTKGSHFNYTQRYPRSFAQWKPECIGVDSGCTKAQMINSYDNSVTYVDHFISSVID
                     QVRDKKAIVFYAADHGESINEREHLHGTPRELAPPEQFRVPMMVWMSDKYLENPANAQ
                     AFAQLKKEADMKVPRRHVELYDTIMGCLGYTSPDGGINENNNWCHIPQTKVAAAN"
     gene            12572..12648
                     /locus_tag="OO357_002640"
     tRNA            12572..12648
                     /locus_tag="OO357_002640"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:12606..12608,aa:Pro,seq:cgg)
     gene            13392..14999
                     /gene="dppA"
                     /locus_tag="OO357_002641"
     CDS             13392..14999
                     /gene="dppA"
                     /locus_tag="OO357_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter substrate-binding
                     protein DppA"
                     /protein_id="HCR5887544.1"
                     /translation="MRISLKKSGMLKLGLSLVAMTVAASVQAKTLVYCSEGSPEGFNP
                     QLFTSGTTYDASSVPLYNRLVEFKIGTTEVIPGLAEKWEVSEDGKTYTFHLRKGVKWH
                     DNKEFKPTREMNADDVVFSFDRQKNAQNPYHKVSGGSYEYFEGMGLPELISEVKKVDD
                     NTVQFVLTRPEAPFLADLAMDFASILSKEYADAMMKAGTPEKLDLNPIGTGPFQLQQY
                     QKDSRIRYKAFDGYWGTKPQIDTLVFSITPDASVRYAKLQKNECQVMPYPNPADIARM
                     KQDKSINLMEMPGLNVGYLSYNVQKKPLDDVKVRQALTYAVNKDAIIKAVYQGAGVSA
                     KNLIPPTMWGYNDDVQDYTYDPEKAKALLKEAGLEKGFSIDLWAMPVQRPYNPNARRM
                     AEMIQADWAKVGVQAKIVTYEWGEYLKRAKDGEHQTVMMGWTGDNGDPDNFFATLFSC
                     AASEQGSNYSKWCYKPFEDLIQPARATDDHNKRVELYKQAQVVMHDQAPALIIAHSTV
                     FEPVRKEVKGYVVDPLGKHHFENVSIE"
     gene            15307..16326
                     /gene="dppB"
                     /locus_tag="OO357_002642"
     CDS             15307..16326
                     /gene="dppB"
                     /locus_tag="OO357_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppB"
                     /protein_id="HCR5887545.1"
                     /translation="MLQFILRRLGLVIPTFIGITLLTFAFVHMIPGDPVMIMAGERGI
                     SPERHVQLLAELGLDKPMWQQYLHYIWGVMHGDLGISMKSRIPVWEEFVPRFQATLEL
                     GVCAMIFATAVGIPVGVLAAVKRGSIFDHTAVGLALTGYSMPIFWWGMMLIMLVSVHW
                     NLTPVSGRVSDMVFLDDSNPLTGFMLIDTAIWGEDGNFIDAVAHMILPAIVLGTIPLA
                     VIVRMTRSSMLEVLGEDYIRTARAKGLTRMRVIIVHALRNAMLPVVTVIGLQVGTLLA
                     GAILTETIFSWPGLGRWLIDALQRRDYPVVQGGVLLVATMIILVNLLVDLLYGVVNPR
                     IRHKK"
     gene            16336..17238
                     /gene="dppC"
                     /locus_tag="OO357_002643"
     CDS             16336..17238
                     /gene="dppC"
                     /locus_tag="OO357_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010426221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter permease DppC"
                     /protein_id="HCR5887546.1"
                     /translation="MSQVTENKVISAPVPMTPLQEFWHYFKRNKGAVVGLVYVAIVLF
                     IAIFANWIAPYNPADQFRDALLAPPAWQEGGSMAHLLGTDDVGRDVLSRLMYGARLSL
                     LVGCLVVVLSLIMGVILGLIAGYFGGLVDNIIMRVVDIMLALPSLLLALVLVAIFGPS
                     IGNAALALTFVALPHYVRLTRAAVLVEVNRDYVTASRVAGAGAMRQMFINIFPNCLAP
                     LIVQASLGFSNAILDMAALGFLGMGAQPPTPEWGTMLSDVLQFAQSAWWVVTFPGLAI
                     LLTVLAFNLMGDGLRDALDPKLKQ"
     gene            17249..18232
                     /gene="dppD"
                     /locus_tag="OO357_002644"
     CDS             17249..18232
                     /gene="dppD"
                     /locus_tag="OO357_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HCR5887547.1"
                     /translation="MALLNVDKLSVHFGDESAPFRAVDRISYSVKQGEVVGIVGESGS
                     GKSVSSLAIMGLIDYPGRVMAEKLEFNGQDLQRISEKERRNLVGAEVAMIFQDPMTSL
                     NPCYTVGFQIMEAIKVHQGGNKSTRRQRAIDLLNQVGIPDPASRLDVYPHQLSGGMSQ
                     RVMIAMAIACRPKLLIADEPTTALDVTIQAQIIELLLELQQKENMALVLITHDLALVA
                     EAAHKIIVMYAGQVVETGDAHAIFHAPRHPYTQALLRALPEFAQDKERLASLPGVVPG
                     KYDRPNGCLLNPRCPYATDRCRAEEPALNMLADGRQSKCHYPLDDAGRPTL"
     gene            18229..19233
                     /gene="dppF"
                     /locus_tag="OO357_002645"
     CDS             18229..19233
                     /gene="dppF"
                     /locus_tag="OO357_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP binding subunit
                     DppF"
                     /protein_id="HCR5887548.1"
                     /translation="MSTQEATSQQPLLQAIDLKKHYPVKKGMFVPERLVKALDGVSFN
                     LERGKTLAVVGESGCGKSTLGRLLTMIETPTGGELYYQGQDLLKHDPQAQKLRRQKIQ
                     IVFQNPYGSLNPRKKVGQILEEPLLINTSLSKEQRREKALSMMAKVGLKTEHYDRYPH
                     MFSGGQRQRIAIARGLMLDPDVVIADEPVSALDVSVRAQVLNLMMDLQQELGLSYVFI
                     SHDLSVVEHIADEVMVMYLGRCVEKGTKDQIFNNPRHPYTQALLSATPRLNPDDRRER
                     IKLTGELPSPLNPPPGCAFNARCRRRFGPCTQLQPQLKDYGGQLVACFAVDQDENPQR
                     "
     gene            complement(19263..20534)
                     /locus_tag="OO357_002646"
     CDS             complement(19263..20534)
                     /locus_tag="OO357_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5887549.1"
                     /translation="MQHNTLSKHNQKLPFTRYDFGWVLLCIGMAIGAGTVLMPVQIGL
                     KGIWVFITAAIIAYPVTWVVQDIYLKTLSESDSCNDYTDIISHYLGKNWGIFLGVIYF
                     LMIIHGIFIYSLSVVFDSASYLKTFGLTDADLSQSLLYKVAIFAVLIAIASGGERLLF
                     KISGPMVVVKVGIIVVFGFAMIPHWNFANITAFPQASVFFRDVLLTIPFCFFSAVFIQ
                     VLNPMNIAYRKREADKVLATRLALRTHRISYITLIAVILFFAFSFTFSISHEEAVSAF
                     EQNISALALAAQVIPGHIIHITSTVLNIFAVLTAFFGIYLGFHEAIKGIILNLLSRII
                     DTKKINSRMLTLAICAFIVITLTIWVSFRVSVLVFFQLGSPLYGIVSCLIPFFLIYKV
                     AQLEKLRGFKAWLILLYGILLCLSPLLKLIE"
BASE COUNT         5040 a         5112 c         5443 g         5212 t
ORIGIN      
        1 gagaatagag gccgatacgg ataccgtagg gtaacgctgt ctcttcatcg agaagggaaa
       61 cagattaacc ataaagctgt tcagcgcctg atgggaaccc tctcacttaa agcagcgatt
      121 aaggtcaagc gataccgctc ttacagagga gaggtagggc aaaccgcccc taatgttctc
      181 caaagagatt tcaaggctac gcggccaaac gagaagtggg ttaccgatgt tactgaattt
      241 gcagtcaatg ggcgcaagct gtatttgtct ccagtaatag atctcttcaa caacgaagtt
      301 atttcttaca gcctttcgga aagaccagtg atgaacatgg ttgagaatat gctcgatcag
      361 gcattcaaaa agcttaatcc tcacgagcat cctgttctgc actctgacca gggatggcag
      421 tatcgtatga gaagatatca aaatatcctt aaagaacatg gtattaaaca aagcatgtcc
      481 agaaaaggca attgtctgga taatgctgtg gtggagtgtt tctttggaac cttaaagtcg
      541 gagtgttttt atcttgatga gttcagtaat ataagcgaac tgaaggatgc tgttacggaa
      601 tatattgaat actataacag cagaagaatt agcctgaaat taaaaggtct gactccaatt
      661 gaatatcgga atcagaccta tatgcctcgt gtataactgt ccaacttttt ggggtcagta
      721 caaagtgagg ctaccctatg cctgaacacc ataaggttag cctcttactc gttggaaatc
      781 aacacagggg gctgggaatg ccgcagaaat atagattact ttctttaata gtgatttgtt
      841 tcacgctttt atttttcacc tggatgataa gagattcact gtgtgaattg catattaaac
      901 aggagagtta tgagctggcg gcgtttttag cctacaaatt gaaagagtaa gagtcttcga
      961 cgggaaatta ttcccgcctt acttacggcg ttgcgcattc tcattgcacc catatttatt
     1021 cttcacaaaa ataataatag attttattac gcgatcgatt atttatttcc tgaaaaaaaa
     1081 taaaaaaatc cccgccaaat ggcagggatc ttagattctg tgcttttaag cagagattac
     1141 aggctggtta cgttaccagc tgccgggcct ttagcgccgc tttcgatggt gaaggacact
     1201 ttctgacctt cgtccagaga tttgtaacca tcgttctgga tagcagagaa gtgtacgaac
     1261 acatctttag agccatcgtc aggagtgatg aagccgaagc ctttgtcagc gttgaaccat
     1321 tttacgatac cagtcatttt accggacata gtgtattacc tttaataatt aagtgtgcct
     1381 ttcggcgata tggcgtgctt tacagatttt gaagcgttaa aggaatgtgc actacgaggg
     1441 gtatcaacga taactcttga agggacttgc cttactacac tgctttaatg gtctgtacgt
     1501 caaaccgttg atgtgcatta agccactcat tggcgggtga tgcaacaatt atttttcata
     1561 tttatgatta atcggttgga aaacggtcct gtcatcagga ccgtaaacag caataaagtg
     1621 gataaaagtc tatttcatca actgcttact taatgccggg ttggcttgaa tcaaacgcat
     1681 caattttagt tcggcacttg aaggcttcac gcgtctggat tcccattcct ttaccatggc
     1741 gactgatacg cccaaaaccc gggcgaaatc atcgattttt aatcctgtcc cttttcataa
     1801 ctgctcaatt tcggtacagg acgttgttct gtgcgtcagg gtaattttct gcgtttcatc
     1861 tttaaaaaca atctgttcca ggctgctcaa cagctcatgc attggatctt tatattccat
     1921 tgaaaactcc tcaaatcaca ctgcgggatc gtgaattgca tcgaagctca ttaagaatag
     1981 tcgggaaaag cagacacagc ggggtgaagt cagggcatgc tctggctatg attcgattca
     2041 gcctgtcatt tagcgtgact tatagccacc taacgatctg ataaatgccg atatatctct
     2101 tatcactcct gttttgtaaa gatttcgcaa atctaacctt ctgcgaaggc gtaaatctct
     2161 ggctacaaaa aaggcaacgg cactatcatt ttttaacaat gaaacaacaa attaaccttg
     2221 tttcattttc tccatgattt taccaggaag attacgtaga aagggtatcc tgcaagcctg
     2281 tcctggacta tccttgtcac gtcagacacg cgtgtgtcgt tgtgcgcttt ttttgggtga
     2341 aaggagtaag aaaatggcga caggaaagtc ctgctctcgc tggtttgcgc ctcttgcggc
     2401 gttattaatg gtagttagcc tgagtgggtg ttttgataaa gaaggcgatc agcgtaaagc
     2461 gtttatcgac ttcctgcaaa atacagtgat gcgtagcggt gaacgtctac caaccctgac
     2521 tgccgatcag aaaaaacagt ttggtccttt tgtctctgat tacgcgattc tgtatggtta
     2581 ttctcagcag gtgaatcagg cgatggattc cggtctgcgc ccggtcgtag acagcgttaa
     2641 cgcaattcgc gtaccgcagg attacgttac gcaaagcggt ccgctgcgtg aaatgaatgg
     2701 ttcgctgggt gtgctggcgc aacaactgca aaatgcgaag ttgcaggccg atgctgcgca
     2761 ctctgcgcta aaacagagcg atgacctgaa accggtattt gatcaggcat tcaccaaagt
     2821 ggtaacgacg ccagctgatg cattgcaacc gttgattccg gcggcacaaa ctttcacgca
     2881 acaactggtg atggttggag actacattgc tcagcaaggt actcaggtga gctttgtcgc
     2941 aaatggcatc cagttcccga cttcacagca ggcaagtgaa tataacaaac tgattgcgcc
     3001 attaccggca caacatcagg cgtttaatca ggcctggact acagcagtta ctgcaaccca
     3061 ataaagagta aaagcccgag cgattatcgg gctttacttc acgcagtcgc agcttagtcc
     3121 gcgacgtgag gattcacaca gttcttctca acctttcctt gtaacgcatc aatcaaatta
     3181 tccacggcac aggcggccat gccataacgc gtctcatggg tggcagatcc aatatgcggt
     3241 actgcgacga cgttggccat tgagagcaac ggcgaatcta cggacagtgg ctcttgttcg
     3301 aagacatcca gcccggcggc gtgaatttcg cctttctgca atgctgcgat cagtgcattt
     3361 tcgtcaacca ccgggccacg tccggcatta atgaaaatgg cggaggattt cattttggcg
     3421 aattgttctg cgccaaacag atgatgcgtc tcatcagtta acggcaggat caggcaaacg
     3481 aaatctgact cttgtaacag agtatccaaa tcgcagtagc gggcgttgaa gcgttcttct
     3541 gcttctttat ggtggcggcg cgcgttatag aggatgggca tgttgaagcc aaagtgcaca
     3601 cgttgtgcca gcgccatgcc gatccgtccc atcccgacaa tgcccagtgt tttatggtga
     3661 acgtcagtgc cgtaccagtc cgggcctatg ctcgcggtcc attcgcctgc ttttacccgt
     3721 tctgctacct ccacaacccg acgagcggta gacaacacca gcgccatcag cgtatcggcg
     3781 acggtttctg ttaatacggt tggcgtgtgc atcagcagaa tttttcgggc ggtaagcgca
     3841 tcgacatcaa aattgtcata gccgacggag atcgttgatg tggcacgcag tttcggcatt
     3901 ttttccagca atgcggcatc tacattctcg tttgaaccca gtaaaccttc agcttctgca
     3961 aaaattgctg cattttgaac gacggtttgt gggctgaggt ttgccacctg gtgaacggtg
     4021 aaatgcgctt gcaggcgttg cagtaaatca tcaggtaagg ctttgtagag gataacggac
     4081 ggcttcatgc ttctctccat tcactgataa ggcgatcacc ttagcctgaa atatgatgaa
     4141 ggaaaactga agaatgccag gtgatttctg catcacctgg catgaaaggg attacagcgg
     4201 gcttaaggta atttctacac ggcggttttg cgccttacct tctgcggtgc tgttgctggc
     4261 gattgggtta gccgggccaa ggccctgagt acggatgcgg ctggcgtcca cgccctgggt
     4321 gatcaacgcg ctggcaacgg aatccgcacg ctgctgggag agacgcatgt tcaggtcgtg
     4381 accccccgtg ctgtcggtat aaccaatcac gttaaccgcc gttttcggat actctttcag
     4441 caccattgcc acgccggtca gggtgttagc gcctgccggt ttcaggggag cgctgctgct
     4501 gtcgaaggtc acattgttcg gcatattgag gataatgtta tccccgctgc gggttacgct
     4561 aacaccagtg ccgcgcattt tgtcacgcag cttcgcttcc tgcacatcca tgtaataacc
     4621 cacgccgccg cccagagctg cgcctgctgc tgcgccaatc agcgcgcctt taccgcgatc
     4681 tttcttcgaa gaagagagcg caccaatacc cgcgcccacg agagagccca gacctgcgcc
     4741 gatagcagat ttgcctgctt cgcgttcgcc ggtgtaaggg ttagttgtgc agccagatac
     4801 cgccagagcg ccactcacta cggcggcaag taagataaac acgtttcttc attgttaatc
     4861 cttaataacc tttttattct ttgtcacggg ttccgtggcg ggagattatg ccgcgtgaac
     4921 atgaagatta ttcctgggaa tactcggaaa tttgtaagta atatttaact gctcaataca
     4981 tctaaccttt caggagtctt cggtttggcc aactcatcct cacgatattc cgttctgact
     5041 gccgcccact gggggcccat gctggttgaa accgacggcg aaaccgtgtt tagctcgcgt
     5101 ggcgcgttag ccacaggaat ggaaaactcc ttgcagagcg cggttcgcga ccaggttcac
     5161 agcaatacgc gggtacgatt tccaatggtg cgcaaaggct ttctcgcgtc accggaaaac
     5221 ccgcaaggca ttcgtgggca ggatgaattt gttcgcgtga gttgggatga ggcgctggca
     5281 ggcggttata ccgggcatct gggggattat tcgaccggtg cggcacaggc gatcatgccg
     5341 tatgtcgtag gtggcagcga agtttatcaa cagcaaacca gttggccgct ggtgctggaa
     5401 catagcgatg tcgtggtgtt gtggagtgct aacccactca atacgctgaa aattgcgtgg
     5461 aatgcatccg atgagcaggg gctttcttac ttttccgcac tgcgtgacag tgggaaaaag
     5521 ctgatctgca ttgatccaat gcgatcggaa accgtcgatt tctttggcga taaaatggag
     5581 tgggtggcac cgcacatggg caccgatgtt gcgctgatgc tggggatcgc ccatacgctg
     5641 gtggaaaatg gttggcacga cgaagcgttt ctggcgcgtt gcaccacagg ttatgccgtc
     5701 ttcgcctctt atttgctggg cgagagtgac ggaatagcga aaaacgccga atgggcggca
     5761 gagatttgtg gtgttggcgc agcgaaaatc cgcgagctgg cggctatttt ccaccaaaat
     5821 accaccatgc tgatggcagg ctggggaatg cagcgccaac agtttggtga gcaaaaacac
     5881 tggatgatcg tcacgctggc agcaatgttg gggcaaatcg gcacacccgg cggcggtttt
     5941 ggtctttctt accattttgc caatggtggt gaccccacgc ggcgttctgc ggtgctctct
     6001 tccatgcagg gcagcttgcc gggtggctgc gatgcggtgg ataaaatccc tgttgcccgc
     6061 attgttgaag cactggaaaa ccctggtggc gcatatcaac acaacggtat gaaccgacat
     6121 ttcccggata ttcgttttat ctggtgggcg ggcggtgcca actttactca tcatcaggat
     6181 accaatcgtc tgatccgtgc ctggcaaaaa ccggagctgg tggtgatctc tgaatgcttc
     6241 tggacggcgg cggcgaaaca cgcggatatt gttctgcctg cgactacctc ttttgagcgt
     6301 aatgatctca ccatgaccgg tgattacagt aatcagcatc tggtgccgat gaagcaagtg
     6361 gtgccgccac gctatgaagc gcgtaatgat tttgatgttt ttgccgagtt gagtgaacgt
     6421 tgggagaagg gcggctatgc gcggtttacg gaaggaaaaa gtcagctgca atggctggaa
     6481 acgttttata acgttgcccg gcagcgcggg gcaagccagc aggttgaatt gccgccattt
     6541 gctgagttct ggcaagccaa ccagttaatt gagatgccgg aaaacccgga cagcgagcgg
     6601 tttattcgct tcgctgattt ttgccgcgat ccgctggcgc atccgttaaa aaccgccagc
     6661 ggcaagattg aaatcttctc acagcgtatt gccgattacg gttacccgga ttgccctggg
     6721 catccaatgt ggctggagcc ggacgaatgg cagggcaatg ccgaaccaga acagttgcag
     6781 gtactttctg cccatccggc gcaccgcctg cacagccagc tgaattacag ttctctgcgc
     6841 gaattgtacg cggtagcaaa ccgtgagcct gtcaccattc atcctgacga tgctcaggcg
     6901 cgcggcataa ccgaagggga tatggtgcgg gtgtggaact cccgtgggca gatccttgcc
     6961 ggagcggtca ttagcgaggg aattaaacct ggcgtgattt gcattcacga aggggcatgg
     7021 ccggatctgg atttaaccgc tgacggtatt tgtaaaaacg gcgcagtgaa cgtgctgacc
     7081 aaagatctcc ccagctcgcg gctggggaat ggctgtgcgg gtaatacgac gctggcatgg
     7141 ctggaaaaat acaacggtcc ggaactgaca cttacagcgt ttgaaccacc ggccagctca
     7201 taatccaggt gggtagttgg gtttcatcct gccatgcgca atcgacaatg tgaaaaccct
     7261 gcgcacggta aaaatctatc gccggttgat ttttttgata aacctccagc atcagatggg
     7321 gatagcgctg ctgcacatac tgcatcagcg ccttaccaat accgcgtctg acggccttcg
     7381 gtgcgacaaa cattgctgcc agaaatcggc cttccataat gctgacaaaa ccgagaagct
     7441 taccgtcctc ttcccagacc cagttttgcg cgttggcaag ataggcatcc cgcaccagcg
     7501 gaatgcagtc acgccagtaa ttcgctttta taaagggatg cccccaggtt gtactttcca
     7561 gccacagttc gaggatcgcg gggagttctg aacgttgcgc ttcccgaatc atggtttatt
     7621 tcccagatag caacagcagc caaccacatg atcattcacc agcccacatg cctgcataaa
     7681 ggagtaacag attgtggtgc cgacaaactt aaaaccacgt tttttcagtg ccttagatag
     7741 ggcgtcggag gcggacgtag atgtgggaat ttcgctcaac gttgtggctt gtgtcacctg
     7801 tggctgatga tttacaaacg accagacaaa gtcgggaaac ggttcgccgt tctgttccat
     7861 ttgcaggtac gcccgcgcat taccaataat tgcctgaatt ttccctcgat ggcggataat
     7921 cccggcgtcc tgtaccagtc tttcgacatc ctcttcctgc attgctgcga ccttcaccgg
     7981 atcgaactga tgaaagtagg cgcgatagtt ttcgcgtttt ttgaggacgg tgatccacga
     8041 taacccagcc tgctgccctt caaagcagat catttcgaac agttttttac tgtcagtttc
     8101 aggcacgccc cactcattat catggtaggc aatataaagc gggccctgac tcacccagcc
     8161 gcaacgttcc atactttccc tcgctatgta cccgatcgta aaaatttcag ccgcttttct
     8221 tgacgcatcg gtgaaaaagt caatatttaa tacagggcta ctatgcccga tatccttcat
     8281 acaatgacaa taatattgcc gaattcggct cttccctgga gtcgtgttaa tgatcataaa
     8341 aaaaagcggt ggtcgctggc agctaagcct gctggcgagc gtggtaatca gtgccttttt
     8401 tctcaacaca gcttacgcct ggcaacaaga atatatcgtt gatacgcaac ccggacattc
     8461 cacagagcgt tacacctggg atagtgatca tcaacctgat tacaacgata ttttgtcgca
     8521 acgtattcaa agtagccaaa gggcgctggg actggaagtc aatctggcgg aagaaactcc
     8581 tgtggatgtg accagcagta tgagtatggg ctggaatttt cctttatatg aacaggttac
     8641 aaccggcccg gtcgcggcat tacattacga tggcacaacc acctcgatgt ataacgagtt
     8701 tggcgacagt actaccacgc tgaccgatcc gttatggcat gccagcgtga gtagcttagg
     8761 ctggcgtgtt gactcccggc ttggcgatct ccgaccctgg gcgcaaatca gctataacca
     8821 gcaatttggc gagaatatct ggaaggcgca atcaggcctg agccggatga cggcgacaaa
     8881 ccagaacggc aactggctgg atgtcaccgt aggcgctgat atgttgctca atcaaaatat
     8941 tgccgcctat gctgcgctaa ctcaggcaga aaataccact aataatagcg actatctgta
     9001 tacgatgggg gttagcgcca gattttaacg taacagtcac aattgaaacc attgaataac
     9061 aacagttgtg gcgatagtgg gtgctaactt accaaataat aaatttggtg aataattgtc
     9121 gcgtcattca ttcctgaact aaggcatttc attccgttct gatggcattt catgccgttt
     9181 ttccccaagc ataaaatgca cttcgttatg gttgtcggca gagatttttc ctttttatta
     9241 ctgcaggaat actgccatga caccttcaaa ttatcagcgt acccgctggc tgacactcat
     9301 cggtactatc attacccagt ttgcgctggg gtcggtttat acccggagcc tgtttaatgg
     9361 cgcgctttct gccaagctgg atgcgccggt aagccaggtc gctttctctt tcggcttgtt
     9421 aagtctgggg ctggcaattt cgtcttctgt tgcgggcaaa ttgcaggaac gttttggcgt
     9481 taaacgcgtc accgtggctt ccggcatttt gctgggatta ggcttcttcc tgaccgcgca
     9541 ttccaacaac ctgatgatgc tgtggttaag cgccggtgtg ctggtcggtc tggcggatgg
     9601 cgcgggttac ctgctgacgc tctctaactg cgtgaagtgg ttcccggagc gtaaaggtct
     9661 gatctccgcg ttcgctatcg gttcttatgg tctgggtagc ctgggtttca aatttatcga
     9721 cacgcagctg ctcgaaacgg tgggtctgga aaaaaccttt gtgatttggg gagcgattgc
     9781 gctggtgatg attgttttcg gcgcaacgtt aatgaaagac gcaccaaaac aggaagtgaa
     9841 aaccagcaat ggtgtggtgg agaaagatta cacgctggca gagtcgatgc gtaaaccgca
     9901 gtactggatg ttagcggtaa tgttcctgac tgcctgcatg agcggcctgt acgtgattgg
     9961 ggtagcgaaa gatatcgccc aaagtctggc acaccttgat gtggtttccg cagccaatgc
    10021 agtaacggtt atttccatcg ctaacctttc aggtcgtctg gtgctgggca ttctgtctga
    10081 caaaattgcc cgtatccgtg ttatcaccat tggtcaggtg atttcgctgg tgggtatggc
    10141 ggccctgctg tttgcaccat taaatgcagt gacgttcttt gcagcgattg cctgcgtggc
    10201 atttaacttt ggcggcacta ttaccgtctt tccatcactg gtcagtgagt tcttcggcct
    10261 caataacctg gcgaaaaact acggtgtgat ttacctcggt ttcggtatcg gtagcatttt
    10321 tggttcgatt atcgcctcac tgtttggcgg tttctatgtg accttctacg tgattttcgc
    10381 cctgctgatt ctgtcattgg cgctttctac gacgattcgt cagccagagc agaaaatgtt
    10441 gcgtgaggcg catggctccc tttaatgctc agccgcgagc tactgggctt cagcttccgt
    10501 gtgcatcaaa cgcatcaacg aaatgtataa acaatgactc gtaaaatttg ggccagttaa
    10561 ctggcccttt ttactgcttt ttgtaacgct aaagtctctt ttcaaacttg catttttgta
    10621 aatttgtgct tcatgcacac tctttcccca cactttttcg ctttgctgtg gtctacttat
    10681 tcgcgcgtgt agattttact tatctgacta cctccgcact tttcccctgc cgggcctgaa
    10741 aagccgctaa gcagggtgtt atcacctgtt tgtccagggt ttgtttgcat gagatacatc
    10801 aaatcgatta cacagcagaa gctgagcttt ttgcttgcaa tctatattgg cctttttatg
    10861 aatggcgcgg ttttttatcg ccgcttcggc agctatgcgc acgattttac cgtctggaaa
    10921 ggcgtttctg ctgttgttga actggccgcc accgtactgg tgaccttctt tttactacgt
    10981 cttctatcgc tgtttggccg ccgcagctgg cgtattctgg cgtcgctggt ggtgctcttt
    11041 tccgcaggtg ccagctatta catgaccttc cttaatgtgg tcattggtta tggcatcatc
    11101 gcttccgtca tgaccaccga tatcgacctg tcaaaagaag ttgttggtct gaactttatt
    11161 ctctggttaa tcgccgttag tgcattgcct cttatcctta tctggaataa ccgctgtcgc
    11221 tacaccttgc tccgacaact gcgaacccca gggcagcgta ttcgcagcct ggtggtcgtc
    11281 gtactggcgg gtattatggt ttgggcaccg attcgtttgc tggatatcca gcagaagaaa
    11341 gtggagaggg cgaccggcgt tgatttgccg agttatggcg gtgtcgtagc gaactcttat
    11401 ctgccatcaa actggctttc tgcgttgggg ctgtatgcct gggcgcgggt cgatgaatct
    11461 tccgataata attcattgct taatccggcg aagaagttta cataccaggc tccacaaaac
    11521 gttgatgata cttatgtcgt gtttatcatc ggtgaaacca cgcgttggga ccatatgggt
    11581 attttcggct atgagcgtaa taccacgccg aaactggccc aggagaaaaa tctggcggcg
    11641 ttccgtggtt actcctgtga taccgcaacc aaactctcac tgcgttgcat gtttgtacgt
    11701 caggggggcg cggaagataa tccgcagcgc acattaaaag aacagaacat tttcgcggtt
    11761 ctgaagcagt taggattcag ttctgacctc tacgctatgc agagcgaaat gtggttctac
    11821 agcaacacga tggcagacaa cattgcttat cgtgagcaga ttggtgcgga gccacgtaat
    11881 cgtggcaagc cggtagatga tatgttgctg gtagacgaaa tgcagcaatc gctagggcgc
    11941 aacccggatg gtaagcatct gatcattctg cataccaaag gttcgcattt taactacacc
    12001 cagcgttatc cgcgtagctt cgcgcagtgg aagccggaat gtattggtgt tgatagcggc
    12061 tgtaccaaag cgcagatgat caactcctat gacaactcgg tgacctatgt ggatcacttt
    12121 atctccagcg tgattgatca ggttcgcgat aagaaagcga ttgtgttcta cgcagctgac
    12181 cacggtgagt caattaatga acgcgagcac ctgcacggca cgccgcgtga actggcaccg
    12241 ccggagcagt tccgcgtacc gatgatggtc tggatgtcag ataaatatct ggaaaatccg
    12301 gccaatgcgc aggcgtttgc gcagctgaaa aaagaagccg acatgaaagt gccacgccgt
    12361 cacgtagagc tgtacgacac catcatgggt tgtcttggct atacttcacc ggatggtgga
    12421 attaacgaaa acaacaactg gtgtcacatc ccgcagacaa aagtggcagc ggctaactaa
    12481 gcggcctgct gactttctcg ccgatcaaaa ggcattttgc tattaaggga ttgacgaggg
    12541 cgtatctgcg cagtaagatg cgccccgcat tcggtgattg gcgcagcctg gtagcgcact
    12601 tcgttcggga cgaaggggtc ggaggttcga atcctctatc accgaccaaa ttcgaaaagc
    12661 ctgctcaacg agcaggtttt tttgcatttg tagctcatga ggatgagaac ctccgggggc
    12721 agggaggctc gactcgacgt aggcctgata agacgcgcca gcgtcgcatc aggcaacggc
    12781 accgcactta ttgtcggatg cggcgcgacc gccttattcg accaacacac cacacatttt
    12841 cctgccaact ttgcgcccca tatctcacta tccattaacg gatttgtgac agaaaccatt
    12901 gtcttttttt atatttgctt aaatcttttt tgcgttactt atggctggcg ctggcatttt
    12961 ccgctgtgcg ttttagcgtt catggtatta atcgctcaga tatccacctt ttcctcagat
    13021 ttttttcatt aaatgcataa aacttaatca ccaaatgttg ctaaatatta agctgattgt
    13081 tctggttatg ttggagtagc acaaatttct ctgctgcaaa tagctgtttg aagttttttt
    13141 aatctttgtt tgtggattct caccgttggt gtaaatcccg gttggctgta ttgacgtttt
    13201 cacattctgt tgacagattg taggtcacga ggggcatttt atggaggatc cgcactgtta
    13261 cactgatgtt aattagtacg gcatccccac ctcataacgt tgacccgacc gggcaaaaaa
    13321 caaaaaaggt caggcagcga caacccactg caaagggtta aaacaacaaa catcacaatt
    13381 ggagcagaat aatgcgtatt tccttgaaaa agtcagggat gctgaagctt ggtctcagcc
    13441 tggtggctat gaccgtcgca gcaagtgttc aggctaaaac tctggtttat tgctcagaag
    13501 gatctccgga agggtttaac ccgcagctgt ttacctccgg caccacctat gacgcctctt
    13561 ccgtcccgct ttataaccgc ctggttgaat ttaaaatcgg caccaccgaa gtgatcccgg
    13621 gcctcgctga aaagtgggaa gtcagcgaag acggtaaaac ctataccttc catctgcgta
    13681 aaggtgtgaa gtggcacgac aataaagaat tcaaaccgac gcgcgaaatg aacgccgatg
    13741 acgtggtgtt ctcgttcgat cgtcagaaaa acgcgcaaaa cccgtaccat aaagtttctg
    13801 gcggcagcta cgaatacttc gaaggtatgg gcttgccgga gctgatcagc gaagtgaaaa
    13861 aggtggacga taacaccgtt cagtttgtgc tgactcgacc ggaagcgccg ttcctcgctg
    13921 acctggcaat ggacttcgcc tctattctgt caaaagaata tgctgacgcg atgatgaaag
    13981 ccggtacacc ggaaaaactg gacctcaacc caatcggaac cggtccgttc cagttacagc
    14041 agtaccaaaa agattcccgt atccgctaca aagcgtttga tggctactgg ggcaccaaac
    14101 cgcagatcga tacgctggtc ttctctatta cccctgatgc ttccgtgcgt tacgcgaaat
    14161 tgcagaagaa tgaatgccag gtgatgccgt acccgaaccc ggcagacata gctcgcatga
    14221 agcaggataa atccatcaac ctgatggaaa tgccggggct gaacgtcggc tacctctcgt
    14281 ataacgtgca gaaaaaaccg ctggatgacg tgaaagttcg ccaagcacta acctacgcgg
    14341 tgaacaaaga cgcgatcatc aaagcggttt atcagggcgc gggtgtatca gcgaaaaacc
    14401 tgatcccgcc aaccatgtgg ggctataacg acgacgttca ggattacacc tacgatcctg
    14461 aaaaagcgaa agccttgctg aaagaagcgg gtctggaaaa aggtttctcc atcgacctgt
    14521 gggcgatgcc ggtacaacgt ccgtataacc cgaacgctcg ccgcatggcg gagatgattc
    14581 aggcagactg ggcgaaagtg ggcgtgcagg ccaaaatcgt cacctacgaa tggggtgagt
    14641 acctcaagcg tgcgaaagat ggcgaacatc agacggtaat gatgggctgg actggcgata
    14701 acggggaccc ggataacttc ttcgccaccc tgttcagctg cgccgcctct gaacaaggtt
    14761 ctaactactc aaaatggtgc tacaaaccgt ttgaagatct gattcaaccg gcgcgtgcta
    14821 ccgacgacca taacaaacgc gttgaactgt acaagcaagc gcaggtggtg atgcacgatc
    14881 aggctccggc actgatcatc gctcactcca ccgtgtttga accggtacgc aaagaagtca
    14941 aaggctatgt ggttgatcca ttaggcaaac atcacttcga aaacgtctct atcgaataat
    15001 taaaagccat acaagactga tggcaaaggc aaaactgcct gatgtgctcc gcttatcagg
    15061 cctacgaaaa ttctgcaatg tattgaattt gcacgatttt gtaggccgga taaggcgtta
    15121 acgccgcatc cggcataaac aaagcgcact ttgtcaacaa tctgtatacc cggtggcgct
    15181 gtgcctctgc gtgcagcgcc atccggcagc aatacttcat tctctgtccg acaagggcgg
    15241 gaattgattt gtgagcaata cagacacgca gttccaggct gcgagtcact atagagaatc
    15301 cgggttatgt tgcagtttat tctccgacgt ttgggactcg tcatccccac gtttatcggt
    15361 attacccttc tcacatttgc ctttgtccac atgatcccgg gcgatccggt gatgatcatg
    15421 gcaggcgaac gtgggatctc ccctgagcgt cacgtgcagc tgctggctga actcggctta
    15481 gataaaccga tgtggcagca gtatctccat tacatttggg gcgtaatgca cggcgatcta
    15541 ggcatttcaa tgaaaagccg aattccagta tgggaagagt tcgtgccgcg ctttcaggcc
    15601 acgctggaac ttggcgtctg cgcgatgatt tttgctacgg cagtcggtat tccggttggt
    15661 gtgctggctg cggttaaacg cggttccatt ttcgatcaca ctgcagttgg cctggcgctg
    15721 accggctact cgatgccgat cttctggtgg ggcatgatgc tgatcatgct ggtttcggtg
    15781 cactggaacc tgacgcccgt ctccggtcgc gtgagcgata tggtgttcct cgatgactcc
    15841 aatccgttaa ccggttttat gctaatcgac accgccatct ggggtgaaga cggcaacttt
    15901 atcgatgccg tcgcccatat gatcttgccc gccattgtgc tgggtactat tccgctagcg
    15961 gtcattgtgc gtatgacacg ctcctcgatg ctggaagtgc tgggcgagga ttacatccgc
    16021 accgcgcgcg ccaaagggct aacccgcatg cgggtgatta tcgtccatgc gctgcgtaac
    16081 gcgatgctgc cggtagtgac cgttatcggc ctccaggtgg gaacattgct ggcgggggcg
    16141 attctgaccg aaaccatctt ctcgtggccc ggtctggggc gctggttgat tgacgcactg
    16201 caacgccgcg actatccggt agtgcagggc ggcgtattgc tggtggcgac gatgattatc
    16261 ctcgtcaact tgctggtcga tctgctgtac ggcgtggtga acccgcgtat tcgtcataag
    16321 aagtaagggg acatcatgtc acaggttact gaaaataaag tgattagcgc accggtgccg
    16381 atgaccccgt tacaggagtt ctggcactat tttaaacgca acaaaggcgc ggtcgtcggg
    16441 ctggtttacg tcgccatcgt gctgttcatc gcgatctttg ccaactggat tgcgccttat
    16501 aacccggcgg atcagttccg cgatgcgctg ctcgccccgc cagcctggca ggaaggcggc
    16561 agtatggcac acttgctggg caccgatgac gtcggtcgtg atgtgctgtc gcggctgatg
    16621 tacggcgcgc gtttatcgct gctggttggc tgtctggtgg tggtgttgtc gctgattatg
    16681 ggcgttattc tcggcctgat cgccggttac ttcggcggcc tggttgataa cattatcatg
    16741 cgcgtggtcg atatcatgct ggcgctgcca agtctgctgt tggcactggt gctggtggcg
    16801 attttcggcc cgtcgattgg taacgccgcg ctggcgctga ccttcgttgc cttgccgcac
    16861 tatgtgcgct taacccgcgc cgccgtgctg gtggaggtga atcgcgatta cgtcaccgcc
    16921 tctcgcgtgg caggtgccgg ggcgatgcgc cagatgttta ttaacatctt cccgaactgc
    16981 cttgcgccgc tgattgttca ggcgtcgctc ggtttctcta acgccattct cgatatggct
    17041 gctcttggtt tcctcggcat gggggcacag ccgccaacgc ctgagtgggg caccatgctc
    17101 tccgacgtgt tgcagttcgc gcaaagcgcc tggtgggtcg tgaccttccc tggtctggcg
    17161 atcctgttga cggtgctggc atttaacctg atgggtgacg gtctgcgtga cgcgctcgat
    17221 cccaaactaa agcagtaaga ggttcgagat ggcgttatta aatgtagata aattatcggt
    17281 gcatttcggc gacgaaagcg caccgttccg cgccgtagac cgcatcagct acagcgtaaa
    17341 acagggcgaa gtggtcggga ttgtgggtga gtccggctcc ggtaagtcgg tcagttcact
    17401 ggcgattatg gggctgattg attatccggg ccgcgtaatg gcagaaaaac tggagtttaa
    17461 cggccaggat ttgcagcgta tctcagaaaa agagcgccgc aacctggtgg gtgccgaagt
    17521 ggcgatgatc ttccaggacc cgatgaccag ccttaacccg tgctacaccg tgggtttcca
    17581 gattatggaa gcgattaaag tgcatcaggg cggcaacaaa agtacccgcc gtcagcgggc
    17641 gattgacctg ctgaatcagg tcggtattcc cgatccggct tcgcgtctgg atgtttaccc
    17701 gcatcagctt tccggcggca tgagccagcg cgtgatgatc gccatggcga ttgcctgtcg
    17761 gccaaaactg ctgattgccg atgaaccgac caccgcgctg gacgtgacca ttcaggcgca
    17821 aatcatcgaa ctactgctgg agctacagca gaaagagaac atggcgctgg tgttaattac
    17881 ccatgacctg gcgctggtgg cggaagctgc gcataaaatc attgtgatgt atgcaggtca
    17941 ggtagtagaa acgggcgatg cacacgccat cttccatgcg ccgcgtcacc cgtatactca
    18001 ggcattgctg cgtgcgctgc cagaatttgc tcaggacaaa gaacgtctgg cgtcgttgcc
    18061 aggtgttgtt cccggcaagt atgaccgccc gaacggctgc ctgcttaacc cgcgctgccc
    18121 ctatgccact gacagatgtc gcgctgaaga accggcgctg aatatgctcg ctgatgggcg
    18181 tcagtccaaa tgccattacc cacttgatga tgccgggagg cccacactat gagtacgcaa
    18241 gaggccacct cgcaacaacc gctgttgcag gctatcgacc tgaaaaaaca ttatccggtg
    18301 aagaaaggca tgttcgtgcc ggaacgtctg gtgaaggcgc tggacggcgt ttcgtttaac
    18361 cttgaacgtg gcaaaacgct ggcagtagtg ggcgaatctg gctgcggtaa atcgaccctc
    18421 ggtcggttgc tgacgatgat cgaaacgccc accggcggcg agctgtatta tcaggggcag
    18481 gatctgctca agcacgatcc gcaggcgcag aagctgcgtc ggcagaaaat ccagatcgtc
    18541 ttccagaatc cctacggttc gctgaatccg cgtaagaaag ttgggcaaat tctcgaagag
    18601 ccgctgctta ttaacaccag cttaagcaaa gaacagcgtc gggaaaaagc cctgtcgatg
    18661 atggcaaaag tcggcctgaa aaccgagcac tatgaccgct atccgcacat gttttccggc
    18721 ggacaacgtc agcgtatcgc tattgcccgt ggtctgatgc tcgacccgga tgtggtgatt
    18781 gccgatgagc cggtttctgc gctggacgtc tcagtgcgtg cgcaggtgct gaatctgatg
    18841 atggatttgc agcaggagtt ggggctttct tatgtcttta tctcccacga cctgtcggtt
    18901 gtggagcaca ttgctgatga agtgatggtg atgtacctag gccgctgcgt ggagaaagga
    18961 acgaaggacc aaatcttcaa taacccgcgc catccgtaca cccaggcgct gctctccgcg
    19021 acgccacgcc tgaacccgga cgatcgtcgt gaacgcatca aactcaccgg tgaactgcca
    19081 agcccgctga atcctccgcc gggttgcgcc ttcaacgccc gctgtcgtcg gcgcttcggc
    19141 ccctgcaccc agttgcagcc gcagctaaaa gactacggag gtcaactggt agcttgtttt
    19201 gctgttgatc aggatgaaaa tccgcagcgt taactcacaa aaccggggcc atgcgctccg
    19261 gtttactcaa tcagcttcaa cagtggcgac aagcatagca aaatgccgta aagcagaatc
    19321 agccaggctt taaatccgcg aagtttttcc agttgtgcga ctttatagat caggaaaaac
    19381 ggaatgaggc acgacacaat gccatataac gggcttccca actgaaagaa caccagcact
    19441 gatacacgaa acgaaaccca aatcgtcaac gtaatgacga taaaagcgca gatcgccaga
    19501 gtcagcatgc gtgagttaat tttcttggta tcaataattc ggcttaacag attgagaata
    19561 atgcctttaa tagcctcgtg gaaaccgaga taaatgccaa agaatgcggt cagtacggca
    19621 aagatattca gcaccgtaga ggtgatatga atgatatgtc cagggatcac ctgcgcggcc
    19681 agtgccagtg ccgagatatt ttgttcaaag gcagaaacgg cttcttcgtg gctaattgag
    19741 aaggtaaacg aaaaggcaaa aaacaggatc accgcgatga gcgtgatata actaattcgg
    19801 tgggtacgca gcgcgagccg ggttgccagt actttatctg cttcccgttt acgataggca
    19861 atattcattg ggttaagtac ctgaataaat accgcagaaa agaagcaaaa tggaatggta
    19921 agcaggacat cgcggaaaaa gacggaggct tgcgggaagg cggttatatt ggcgaaattc
    19981 cagtgcggga tcatcgcaaa accgaacacg acaataatcc ctactttgac caccaccatt
    20041 ggcccagaaa tcttaaacag taatcgttca ccaccagacg caatcgctat cagtacggcg
    20101 aaaatagcga ctttataaag tagagattgt gaaagatcgg catcggttaa accgaaggtt
    20161 ttcaggtacg aggcgctgtc gaaaaccacg gagagagagt agataaaaat cccgtggata
    20221 atcatcaaaa agtagataac cccgaggaaa attccccagt tcttccccag gtaatgactg
    20281 ataatatcgg tgtagtcatt acaggaatcg ctttcagaaa gggtttttaa ataaatgtcc
    20341 tgcactaccc aggtgacagg ataagcaatg atcgctgcgg taataaatac ccaaattccc
    20401 ttcaagccaa tttgtactgg catcagcacg gttccggcac caatcgccat gcctatgcat
    20461 aataaaaccc agccgaagtc gtagcgtgta aacggcaatt tctgattgtg tttcgatagt
    20521 gtgttgtgct gcataaagtc ctgtccagaa agaagtcggc ctcgcaaaaa tgaataaacc
    20581 cagtttctct tgcgcaacag gtggagaagt cgaatacgca gtatgtaaaa aatgaggcaa
    20641 cagattcact ggggaggtcg cgaggaggcg caattctaca aaattgcgca tttcactacc
    20701 agtctggagt cagcccggat atgaactgca actattgcaa ataaagaaat taagctggaa
    20761 ggcatcgcgc aatgaggaag attgccgggt gctgacagta aaaagca
//
LOCUS       DAJTRC010000066        20779 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.066, whole genome shotgun sequence.
ACCESSION   DAJTRC010000066 DAJTRC010000000
VERSION     DAJTRC010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20779
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.066"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..579
                     /locus_tag="OO357_002647"
     CDS             <1..579
                     /locus_tag="OO357_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5887550.1"
                     /translation="GYAINAALTEKRIKAVVSITGVNIGRLFREGFSNYDPIGVLNAM
                     ASQRAKEARGGELQINELLPASLDAAKAHGLTERDVYEATDYYKTPRGQQPGGATKML
                     FSHAQKTLAWDAFAFTEVLLTQPVMVVVGEKVGAFGAYRDGLEVYGRAMVSQDRQLVS
                     LPDFSHYELYDKPEAVQEALAKVIPFFNTHLG"
     gene            597..1235
                     /locus_tag="OO357_002648"
     CDS             597..1235
                     /locus_tag="OO357_002648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5887551.1"
                     /translation="MKNVLILGAGGQIARHVINQLADKQTIKQTLFARQPAKIHKPYP
                     TNSQIIMGDVLNHAALKQAMQGQDIVYANLTGEDLDIQANSVIAAMKACDVKRLIFVL
                     SLGIYDEVPGKFVEWNNAVIGEPLKPFRRAADAIEASGLEYTILRPAWLTDEDIIDYE
                     LTSRNEPFKGTIVSRKSVAALITDIIDKPEKHIGENIGINQPGTDGDKPFFM"
     gene            1526..2254
                     /gene="mpaA"
                     /locus_tag="OO357_002649"
     CDS             1526..2254
                     /gene="mpaA"
                     /locus_tag="OO357_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide amidase MpaA"
                     /protein_id="HCR5887552.1"
                     /translation="MTVTRSRAERGAFPPGTEHYGRSLLGAPLIWFPAPAASRESGLI
                     LAGTHGDENSSVVTLSCALRTLTPSLRRHHVVLCVNPDGCQLGLRANANGVDLNRNFP
                     AANWKEGETVYRWNSAAEERDVVLLTGDKPGSEPETQALCQLIHRIQPAWVVSFHDPL
                     ACIEDPRHNELGEWLAQSFELPLVTSVGYETPGSFGSWCADLNLHCITAEFPPISSDE
                     ASEKYLFAMANLLRWHPKDAIRPS"
     gene            complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO357_002650"
     CDS             complement(2229..3194)
                     /gene="ycjG"
                     /locus_tag="OO357_002650"
                     /EC_number="5.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707230.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-Ala-D/L-Glu epimerase"
                     /protein_id="HCR5887553.1"
                     /translation="MRIVKVFEEAWPLHTPFVIARGSRSEARVVVVELEEEGIKGTGE
                     CTPYPRYGESDASVMAQIMSVVPQLEKGLTREELQKILPAGAARNALDCALWDLAARK
                     QQQSLADLIGITLPETVITAQTVVIGTPDQMANSASTLWQAGAKLLKVKLDNHLISER
                     MVAIRTAVPDATLIVDANESWRAEGLAARCQLLADLGVAMLEQPLPAQDDAALENFIH
                     PLPICADESCHTRSNLKALKGRYEMVNIKLDKTGGLTEALALATEARAQGFSLMLGCM
                     LCTSRAISAALPLVPQVSFADLDGPTWLAVDVEPALQFTTGELHL"
     gene            3313..3819
                     /gene="tpx"
                     /locus_tag="OO357_002651"
     CDS             3313..3819
                     /gene="tpx"
                     /locus_tag="OO357_002651"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol peroxidase"
                     /protein_id="HCR5887554.1"
                     /translation="MSQTVHFQGNPVTVANSIPQAGSKAQTFTLVAKDLSDVTLGQFA
                     GKRKVLNIFPSIDTGVCAASVRKFNQLATEIDNTVVLCISADLPFAQSRFCGAEGLNN
                     VITLSTFRNAEFLQAYGVAIADGPLKGLAARAVVVIDENDNVIFSQLVDEITTEPDYE
                     AALAVLKA"
     gene            complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO357_002652"
     CDS             complement(3863..5404)
                     /gene="tyrR"
                     /locus_tag="OO357_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator TyrR"
                     /protein_id="HCR5887555.1"
                     /translation="MRLEVFCEDRLGLTRELLDLLVLRGIDLRGIEIDPIGRIYLNFA
                     ELEFESFSSLMAEIRRIAGVTDVRTVPWMPSEREHLALSALLEALPEPVLSVDMKSKV
                     DMANPASCQLFGQKLDRLRNHTAAQLINGFNFLRWLESEPQDSHNEHVVINGQNFLME
                     ITPVYLQDENDQHVLTGAVVMLRSTIRMGRQLQNVAAQDVSAFSQIVAVSPKMKHVVE
                     QAQKLAMLSAPLLITGDTGTGKDLFAYACHQASPRAGKPYLALNCASIPEDAVESELF
                     GHAPEGKKGFFEQANGGSVLLDEIGEMSPRMQAKLLRFLNDGTFRRVGEDHEVHVDVR
                     VICATQKNLVELVQKGVFREDLYYRLNVLTLNLPPLRDCPQDIMPLTELFVARFADEQ
                     GVPRPKLAADLNTVLTRYAWPGNVRQLKNAIYRALTQLDGYELRPQDILLPDYDAATV
                     AVGEDAMEGSLDEITSRFERSVLTQLYRNYPSTRKLAKRLGVSHTAIANKLREYGLSQ
                     KKNEE"
     gene            complement(5552..6613)
                     /locus_tag="OO357_002653"
     CDS             complement(5552..6613)
                     /locus_tag="OO357_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HCR5887556.1"
                     /translation="MTEPLKPRIDFDGPLEVEQNPKFRAQQTFDENQAQNFAPATLDE
                     AQEEEGQVEAVMDAALRPKRSLWRKMVMGGLALFGASVVGQGIQWTMNAWQTQDWVAL
                     GGCAAGALIIGAGVGSVVTEWRRLWRLRQRAHERDEARDLLHSHGTGKGRAFCEKLAQ
                     QAGIDQSHPALQRWYASIHETQNDREVVSLYAHLVQPVLDAQARREISRSAAESTLMI
                     AVSPLALVDMAFIAWRNLRLINRIATLYGIELGYYSRLRLFKLVLLNIAFAGASELVR
                     EVGMDWMSQDLAARLSTRAAQGIGAGLLTVRLGIKAMELCRPLPWIDDDKPRLGDFRR
                     QLIGQVKETLQKGKTPSEK"
     gene            complement(6610..8007)
                     /locus_tag="OO357_002654"
     CDS             complement(6610..8007)
                     /locus_tag="OO357_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HCR5887557.1"
                     /translation="MKRLKNELNALVNRGVDRHLRLAVTGLSRSGKTAFITAMVNQLL
                     NIHAGARLPLLSAVREERLLGVKRIPQRDFGIPRFTYDEGLAQLYGDPPAWPTPTRGV
                     SEIRLALRFKSNDALLRHFKDTSTLYLEIVDYPGEWLLDLPMLAQDYLSWSRQMTGLL
                     NGQRGEWSAKWRMMCEGLDPLAPADENRLADIAAVWTDYLHHCKQQGLHFIQPGRFVL
                     PGDMAGAPALQFFPWPDVDAWDESKLAQADKHTNAGMLRERFNYYCEKVVKGFYKNHF
                     LRFDRQIVLVDCLQPLNSGPQAFNDMRLALTQLMQSFHYGQRTLFRRLFSPVIDKLLF
                     AAMKADHVTVDQHANMVSLLQQLIQDAWQNASFERISMDCLGLASVQATTSGIIDVNG
                     EKIPALRGNRLSDGALLTVYPGEVPARLPGQAFWDKQGFQFEAFRPQVMDVDKPLPHI
                     RLDAALEFLIGDKLR"
     gene            8163..9161
                     /locus_tag="OO357_002655"
     CDS             8163..9161
                     /locus_tag="OO357_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5887558.1"
                     /translation="MSPTIYDIARVAGVSKSTVSRVLNKQTNISPEAREKVLRAIEEL
                     QYQPNKLARALTSSGFDAIMVISTRSTKTTAGNPFFSEVLHAITAKAEEEGFDVILQT
                     SHNPAEDLQKCESKIKQKMIKGIIMLSSPADESFFAQLDKYDIPVVVIGKVEGQYAHV
                     YSVDTDNFGDSIALTDALIESGHQNIACLHAPLDVHVSVDRVNGYKQSLSAHNIAVRD
                     KWIVDGGYTHETALQAARQLLSQSPLPEAVFATDSLKLMSIYRAAAEKNIAIPQQLAV
                     VGYSNETLSFILTPAPGGIDVPTQELGQQSCELLFRLISGKPSPQNITVATHMTLK"
     gene            complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OO357_002656"
                     /pseudo
     CDS             complement(9272..10144)
                     /gene="ompG"
                     /locus_tag="OO357_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006175591.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="monomeric porin OmpG"
     gene            complement(10217..11298)
                     /locus_tag="OO357_002657"
                     /pseudo
     CDS             complement(10217..11298)
                     /locus_tag="OO357_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309924.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OO357_002658"
     CDS             complement(11312..11971)
                     /gene="pgmB"
                     /locus_tag="OO357_002658"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HCR5887559.1"
                     /translation="MKLQGVIFDLDGVITDTAHLHFQAWQQIAAEIGISIDAQFNESL
                     KGISRDESLRRILQHGGKEGDFNPQERAQLAYRKNLLYVHSLRELTVNAVLPGIRNLL
                     AELRAQQIPVGLASVSLNAPTILAALELREFFTFCADASQLKNSKPDPEIFLAACAGL
                     GVPPQACIGIEDAQAGIDAINASGMRSVGIGAGLTGAQLLLPSTDSLTWSRLSAFWQN
                     V"
     gene            complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OO357_002659"
     CDS             complement(11968..14235)
                     /gene="ycjT"
                     /locus_tag="OO357_002659"
                     /EC_number="2.4.1.230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kojibiose phosphorylase"
                     /protein_id="HCR5887560.1"
                     /translation="MTRPVTLSEPHFSQHTLNKYASLMAQGNGYLGLRASHEEDYTRQ
                     TRGMYLAGLYHRAGKGEINELVNLPDILGMEIAINGEVFSLSREAWQRELDFASGELR
                     RSVVWRTSNGAGYTIASRRFVSADQLPLIALEITIMPLDADASVLISTGIDATQTNHG
                     RQHLDETQVRVFGQHLMQGIYTTQDGRSDVAISCCCQVSGDVQQCYTAKERRLLQHTS
                     TQLHAGETVTLQKLVWIDWRDDRQAALDEWGSVSLRQLEMCAQQSYDQHLAASTENWR
                     QWWQKRRITVNGGDAHDQQALDYALYHLRIMTPAHGERSSIAAKGLTGEGYKGHVFWD
                     TEVFLLPFHLFSDPTVARSLLRYRWHNLPGAQEKARRNGWQGALFPWESARSGEEETP
                     EFAAINIRTGLRQKVASAQAEHHLVADIAWAVIQYWQTTGDESFIAHEGMALLLETAK
                     FWISRAVRVNDRLEIHDVIGPDEYTEHVNNNAFTSYMVYYNVQQALSIARQFGCSDDA
                     FIHRAEMFLKELRLPEIQPDGVLPQDDSFMTKPAINLAKYKAAAGKQTILLDYSRAEV
                     NEMQILKQADVVMLNYMLPEQFSSASCLANLQFYEPRTIHDSSLSKAIHGIVAARCGL
                     LTQSYQFWREGTEIDLGADPHSCDDGIHAAATGAIWLGAIQGFAGVSVRDGELHLNPA
                     LLEQWQQLSFPLFWQGCELQVTLDAQRIAIRTSAPVSLRLDGQLISVAEESVFCLGDF
                     ILPFNGTATTHQEDE"
     gene            complement(14232..14855)
                     /locus_tag="OO357_002660"
     CDS             complement(14232..14855)
                     /locus_tag="OO357_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001529947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5887561.1"
                     /translation="MTNGVLGEIYVTTARALRRCGVPGWGVFTNKELQGGGLLIDIGI
                     HMLDAAMYVLGFPAVKSVNAHSFQKIGTQKSCGQFGEWDPATYSVEDSLFGTIEFHNG
                     GILWLETSFALNIREQLIMNVSFCGDKAGATLFPAHIYTDNNGELMTLMQREMADDNR
                     HLRSMEAFINHVQGKPVMIADAEQGYIIQQLVAALYQSAETGTRVEL"
     gene            complement(14852..15274)
                     /locus_tag="OO357_002661"
     CDS             complement(14852..15274)
                     /locus_tag="OO357_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005084158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HCR5887562.1"
                     /translation="MTSSPLRVAIIGAGQVADKVHASYYCTRNDLELVAVCDSHLSQA
                     QALAEKYGNASVWDDPQAMLLAVKPDVVSVGSPNRFHYEHTLMALEAGCHVMCEKPPA
                     MTTEQAWEMCDTARKLGKVLAYDFHHRLRSIRNSCVNR"
     gene            complement(15296..16071)
                     /locus_tag="OO357_002662"
                     /pseudo
     CDS             complement(15296..16071)
                     /locus_tag="OO357_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415830.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
     gene            complement(16089..17141)
                     /locus_tag="OO357_002663"
     CDS             complement(16089..17141)
                     /locus_tag="OO357_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HCR5887563.1"
                     /translation="MKKLVATAPRVAALVEYEDRAILANEVKIRVRFGAPKHGTEVVD
                     FRAASPFIDEDFNGEWQMFTPRPADAPRGIEFGKFQLGNMVVGDIIECGSDVTDYAVG
                     DSVCGYGPLSETVIINAVNNYKLRKMPQGSSWKNAVCYDPAQFAMSGVRDANVRVGDF
                     VVVVGLGAIGQIAIQLAKRAGASVVIGVDPIAHRCDIARRHGADFCLNPIGTDVGKEI
                     KTLTGKQGADVIIETSGYADALQSALRGLAYGGTISYVAFAKPFAEGFNLGREAHFNN
                     AKIVFSRACSEPNPDYPRWSRKRIEETCWELLMNGYLNCEDLIDPVVTFANSPESYMQ
                     YVDQHPEQSIKMGVTF"
     gene            complement(17172..18014)
                     /locus_tag="OO357_002664"
     CDS             complement(17172..18014)
                     /locus_tag="OO357_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HCR5887564.1"
                     /translation="MATNKRTLSRIGFYCGLALFLIITLFPFFVMLMTSFKSAKEAIS
                     LHPTLLPQQWTLEHYVDIFNPMIFPFVDYFRNSMVVSVVSSVVAVFLGILGAYALSRL
                     RFKGRMTINASFYTVYMFSGILLVVPLFKIITALGIYDTEMALIITMVTQTLPTAVFM
                     LKSYFDTIPDEIEEAAMMDGLNRLQIIFRITVPLAMSGLISVFVYCFMVAWNDYLFAS
                     IFLSSASNFTLPVGLNALFSTPDYIWGRMMAASLVTALPVVIMYALLERFIKSGLTAG
                     GVKG"
     gene            complement(18001..18882)
                     /locus_tag="OO357_002665"
     CDS             complement(18001..18882)
                     /locus_tag="OO357_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HCR5887565.1"
                     /translation="MNRLFSGRSDMPFALLLLAPSLLLLGGLVAWPMVSNIEISFLRL
                     PLNPNIESTFVGVSNYVRILSDPGFWHSLWMTVWYTALVVAGSTVLGLAVAMFFNREF
                     RLRKTARSLVILSYVTPSISLVFAWKYMFNNGYGIVNYLGVDLLHLYEQAPLWFDNPG
                     SSFVLVVLFAIWRYFPYAFISFLAILQTIDKSLYEAAEMDGANAWQRFRIVTLPAIMP
                     VLATVVTLRTIWMFYMFADVYLLTTKVDILGVYLYKTAFAFNDLGKAAAISVVLFIII
                     FAVILLTRKRVNLNGNK"
     gene            complement(18903..20186)
                     /locus_tag="OO357_002666"
                     /pseudo
     CDS             complement(18903..20186)
                     /locus_tag="OO357_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415826.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
     gene            complement(20200..>20779)
                     /gene="ycjM"
                     /locus_tag="OO357_002667"
     CDS             complement(20200..>20779)
                     /gene="ycjM"
                     /locus_tag="OO357_002667"
                     /EC_number="2.4.1.352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415825.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucosylglycerate phosphorylase"
                     /protein_id="HCR5887566.1"
                     /translation="VNWKNNPDGTRSPYEMNVTYMNALSRRESSDEERCTRFILAHAI
                     LLSFPGVPAIYIQSILGSRNDYAGVEKLGYNRAINRKKYHSEEITRELNDEATLRHAV
                     YHELSRLITLRRSHNEFHPDNNFTIDTVNSSVMRIQRSNADGNCLTGLFNVSKNIQHV
                     NITDLHGRDLISEVDILGNEITLRPWQVMWIK"
BASE COUNT         5072 a         5667 c         5052 g         4988 t
ORIGIN      
        1 ggctatgcca ttaatgccgc gctgacagaa aaacgaatta aagctgtagt gagtattacg
       61 ggtgttaata tcgggcgtct ttttcgggaa ggtttcagca attatgatcc gattggcgta
      121 ttgaatgcaa tggcgtcaca acgagcaaaa gaggctcgtg gtggagaatt acagatcaac
      181 gaattacttc ccgccagcct ggatgcagca aaagcccatg gactgaccga acgcgatgtt
      241 tatgaagcaa ctgactacta taaaacacca cgcgggcaac aaccgggagg tgcgacaaaa
      301 atgttgttct cacatgcaca gaaaacgctg gcctgggatg ccttcgcatt tactgaagtg
      361 ttactgaccc agccggtgat ggtagtagtt ggtgaaaaag tcggtgcctt cggtgcttat
      421 cgtgatggac tggaagtcta tggccgggcg atggtttcgc aggatcgtca gttagtttcg
      481 ttacccgatt tctcacatta tgaactatat gacaaaccgg aagcagttca ggaggcgctg
      541 gcaaaagtta ttcctttttt caacacccat ctgggataag tttcatgaga aatattatga
      601 aaaatgtact gattcttggt gctggcggtc agatagcccg ccatgtgatt aatcaactgg
      661 cagataaaca aactattaaa caaactttat ttgcccgtca gccagcaaaa atacacaaac
      721 cctatccaac taatagccag attattatgg gggatgtact gaatcatgca gcactgaagc
      781 aggccatgca gggacaggat attgtgtatg ccaaccttac gggggaagat ttagatattc
      841 aggcaaattc agtgatcgct gcgatgaaag cctgtgatgt taaacgtctg atctttgttc
      901 tttccctcgg tatttatgat gaagttccag gaaaatttgt cgaatggaat aatgcagtta
      961 ttggtgaacc cctaaaacca ttccgcaggg ctgcggatgc aattgaggct tctggactgg
     1021 aatatacaat tcttcgccct gcctggctga ctgatgaaga tattattgat tatgagctta
     1081 cctctcgcaa tgaaccattc aaaggtacga tcgtatcacg taagagtgtc gcagcattaa
     1141 ttacagatat catcgataaa cctgagaaac acattggtga gaatataggc atcaatcaac
     1201 ccgggactga tggcgataag ccattcttca tgtaaatcaa ttatatagat accatcgtac
     1261 tgtactatgt gcagtacgat ttattaaact tattaaggtg aactgatcaa tcagatgcga
     1321 ttaacatgtg tagaaccagt aagaataaaa tacgccatgt atataacatt acgacaattc
     1381 taatatctgc acatcaatga aaatgcaaac aacttccggc aaaaaagtga tacaagatcg
     1441 atgacatagc gttgaagaaa aggatttgat aatccgcaaa atttgcacaa cactgcctgt
     1501 aatacttcct gaaccagaga cgatcatgac cgtaacccgc tcacgcgccg aacgcggcgc
     1561 atttccgccc ggaacagaac attacggacg ttcattattg ggcgcgccgt taatctggtt
     1621 tcctgcccct gcagccagcc gcgaaagtgg tttgattctg gctggcactc acggtgatga
     1681 aaactcttcg gtcgtcacgc tctcctgtgc tctgcggact ttgacacctt ctttgcgccg
     1741 ccatcatgtg gtgctgtgtg tgaatcctga cggctgccag ctggggttac gggccaatgc
     1801 taatggtgtg gatttaaaca gaaactttcc ggcggcgaac tggaaggaag gtgaaacggt
     1861 ttatcgctgg aacagcgccg ctgaagaacg tgatgtcgta ttactgacag gtgacaaacc
     1921 tggctccgaa cctgaaaccc aggcgctgtg ccagcttata caccgcattc aacctgcctg
     1981 ggtggtctca ttccacgatc cactggcttg tattgaagat cccagacaca acgaattagg
     2041 cgaatggctg gcccagtcgt ttgaattgcc tctggtaacc agcgtcggtt atgaaacgcc
     2101 tggctctttc ggcagttggt gtgcagatct caacttgcat tgcatcaccg cagagtttcc
     2161 gcccatctcc tccgacgaag ccagcgaaaa atatctcttt gcgatggcaa acttgctgcg
     2221 ctggcatcct aaagatgcaa ttcgcccgtc gtgaactgaa gcgccggttc cacatctacc
     2281 gccagccagg tcggtccgtc aagatcggcg aaactgacct gcggcaccag cggtaaagcg
     2341 gcgctaatgg cacgagaggt acacaacatg cagcccagca tcagactgaa accttgtgca
     2401 cgcgcttcag tcgccagcgc cagcgcttcc gtcagaccac cggttttatc gagcttaatg
     2461 ttaaccatct catagcgccc tttcagcgcc ttcagattgc tacgagtatg acaactttca
     2521 tcagcacaaa tcggcaacgg atgaataaaa ttctccagcg ccgcatcgtc ctgcgcagga
     2581 agcggttgtt caagcatcgc aacgcctaaa tccgccaata gctggcaacg cgccgccaac
     2641 ccttctgcac gccaggattc atttgcatca acgatcagcg tggcatcggg cacagcggtg
     2701 cgaattgcca ccatccgctc actgataaga tggttatcca gcttcacttt cagtaatttc
     2761 gcgcctgcct gccagagtgt tgatgcacta ttggccatct gatcaggcgt accgatgaca
     2821 accgtctgtg cagtgataac tgtctcggga agcgttatgc cgatcaaatc agccagcgat
     2881 tgctgctgtt ttcgcgccgc gagatcccac aatgcacaat ccagcgcatt acgtgctgcg
     2941 ccggcaggga gaattttttg caactcctcc cgtgtcagcc ctttctctaa ttgcggcacg
     3001 acgctcataa tttgcgccat taccgaggca tcactttctc cataacgcgg atacggcgtg
     3061 cattcgccgg tgcctttaat accctcttct tccagttcaa ccaccaccac gcgcgcttca
     3121 ctgcgacttc cccgggcaat cacaaacggg gtatgtaagg gccaggcttc ctcaaatacc
     3181 ttaacgattc tcatctttac tccttgttgt cgcggtggtc tgcggattaa gcgcaaatag
     3241 cgtttgctgt gttattgaca gttagcataa actaggtgtg acgttaacta tatgtaaaca
     3301 ggaaagataa tcatgtcaca aaccgttcat ttccagggca acccggttac agtcgccaat
     3361 tccatcccgc aggcgggtag caaagcgcag acttttactc tcgtggcaaa agatctgtct
     3421 gacgtcaccc tcggtcagtt tgcgggtaaa cgcaaagtgc tgaacatttt cccgagtatt
     3481 gataccggtg tttgcgccgc atcagtacgt aagtttaacc aactggcaac tgagatcgac
     3541 aacaccgttg tgctgtgtat ctctgccgat ctgccgttcg cccagtctcg tttctgcggc
     3601 gcagaaggtc tgaacaacgt tatcaccctc tccactttcc gtaacgctga atttctgcaa
     3661 gcctacggtg tggcaattgc tgatggccca ctgaaaggtc tggcagcgcg tgccgttgtg
     3721 gttattgacg aaaatgacaa tgtgattttc agccagctgg tggatgaaat caccaccgag
     3781 ccggattacg aagcagctct ggctgtactg aaagcataag ttaatatgcc tgatggtgtt
     3841 gcaccatcag gcatattcgc gcttactctt cgttcttctt ctgactcaga ccatattccc
     3901 gcaacttatt ggcaatcgcg gtatgtgaaa cgccgagacg ttttgccagt ttgcgcgtgc
     3961 tgggataatt gcgataaagc tgggttaata ccgagcgttc aaaacggctg gtgatttcgt
     4021 ccagcgaacc ttccatcgca tcttcgccca cggctaccgt tgcggcgtca taatccggca
     4081 acaaaatatc ctgtggacgc agctcataac cgtccagttg tgtcagtgcg cgatagatag
     4141 cgttctttaa ctgccgcaca tttcccggcc acgcataacg tgtaagtaca gtattcaggt
     4201 cagcggccag tttcggacgc ggcacgccct gctcgtcggc aaagcgggcg acgaacagct
     4261 cagttaacgg cataatgtcc tgcggacagt cacgtagcgg cggcagattg agcgtcaaca
     4321 cgttcagacg ataatagaga tcttcacgga acacgccttt ttgcaccagt tcgaccagat
     4381 tcttctgcgt agcgcaaatc acccgcacat cgacatgcac ctcatggtct tcgccaaccc
     4441 gacggaaagt gccatcatta agaaaacgca gtaatttcgc ctgcatccgt ggtgacattt
     4501 cccctatttc atccaacagc accgaaccac cgttcgcctg ctcaaagaac cctttcttcc
     4561 cttccggagc atgaccaaac agttcactct cgaccgcatc ttctggtata gacgcgcaat
     4621 tcagcgccag gtaaggtttg cccgctctgg ggcttgcctg atggcaggcg taggcaaaaa
     4681 gatctttacc tgtacctgtg tcacccgtaa tcagcagcgg cgcgcttagc atcgccagtt
     4741 tctgcgcctg ttcgacaacg tgcttcattt tcgggctgac ggcgacaatt tggctgaagg
     4801 cgctgacgtc ctgggcggcg acattttgca actggcggcc catacgaatc gttgatcgca
     4861 acatcaccac cgcaccggtc aggacgtgtt gatcattttc atcctgaaga taaacaggcg
     4921 taatctccat caggaaattc tgcccattaa taacgacatg ctcgttatgc gaatcttgcg
     4981 gttcgctttc cagccaacgt aaaaaattaa agccgttaat caattgtgcg gcggtatggt
     5041 tgcgcaggcg atccaatttt tgcccaaaaa gctgacagct cgccgggttc gccatatcca
     5101 ctttgctttt catatcgaca gagagcacag gttcaggcaa cgcctccagt aacgcgctca
     5161 acgccagatg ctcacgttcg gaaggcatcc acgggacagt acgcacatcg gtaacacctg
     5221 caatacggcg tatttcggcc atcagactgc tgaaactctc aaactccagt tcagcaaaat
     5281 tgaggtagat tcgcccaatg ggatcaatct caataccgcg taaatcaatg cctcttagca
     5341 cgagtagatc gagtaattcg cgggtcagac cgagtcggtc ttcacaaaag acttccagac
     5401 gcatgggaac cttcacctga aaaaagaaca attaatatga tgatatgaca ctatttggat
     5461 agcaggaagg tttctgtcaa caatcattga cacaaagacg gaaaaacggg cacttcggcg
     5521 taaagatatc cggtcagcac ccgatattgc attatttttc gctgggcgtt ttgccttttt
     5581 gcagagtttc tttcacctga ccgataagct gacgacggaa atccccaagg cgaggtttgt
     5641 catcgtcaat ccacggcagc gggcggcaaa gctccatagc tttaatcccg agtcgtaccg
     5701 tcagaagtcc agcaccaatt ccctgagctg cgcgggtaga caaacgagca gcgagatctt
     5761 gcgacatcca gtccatcccc acttcgcgca ccagttcgct ggctccggca aaagcgatat
     5821 tcagcaatac cagcttaaac agacgcaaac ggctgtaata ccccagttca atgccataca
     5881 gcgtggcaat gcgattaatt aaacgcagat tgcgccaggc gataaacgcc atatcgacca
     5941 acgccagcgg gctgaccgca atcatcaacg ttgattccgc cgccgaacgg ctgatttcgc
     6001 gccgcgcctg ggcatctaaa actggctgga ccaaatgcgc atacaggctg accacttcac
     6061 gatcgttctg cgtttcatgg attgaggcat accagcgttg cagcgccgga tgcgactgat
     6121 caatacccgc ctgctgcgcc agtttttcgc aaaatgcgcg acctttgccc gtgccgtggc
     6181 tatgcaataa atcacgcgct tcgtcgcgtt catgggcgcg ctgtcgcaag cgccataagc
     6241 gccgccactc tgttaccaca gaacctacgc cagcgccgat aatcaatgcc ccagcggcac
     6301 atccacccag cgctacccag tcctgggttt gccaggcatt cattgtccac tgtataccct
     6361 gcccgacaac gcttgcgcca aacagagcca gcccgcccat caccattttg cgccacaggc
     6421 tgcgtttcgg acgtaacgct gcgtccatta ccgcttcgac ttgcccctct tcttcctgcg
     6481 cttcgtcgag cgtggccggg gcaaaatttt gcgcctgatt ttcgtcaaag gtctgctgcg
     6541 ccctgaattt tggattctgt tcgacctcca gcggaccgtc gaaatcaata cgtggtttta
     6601 acggttcggt catcgcaatt tatctcctat taaaaattcc agcgcggcat caagacgaat
     6661 atgcggcagc ggtttgtcga catccatcac ctgcgggcga aacgcttcaa actggaaccc
     6721 ttgcttgtcc cagaacgcct gaccgggcaa acgcgcagga acttcaccgg ggtaaacggt
     6781 gagaagcgca ccatcgctga gccggttacc gcgcaatgcc ggaattttct cgccgttgac
     6841 gtcaataatg ccgctggtgg tcgcctgcac cgaggccagc cccaggcaat ccatactaat
     6901 cctttcaaac gacgcatttt gccaggcatc ctgaatcagt tgttgcagta atgaaaccat
     6961 attggcgtgc tgatcgaccg ttacatggtc cgctttcatc gcggcaaaca gcagtttgtc
     7021 gataaccggc gagaataagc ggcgaaacag cgtgcgctgc ccgtagtgga aactttgcat
     7081 cagctgcgtc agtgccagac gcatatcatt aaatgcctgt ggcccactgt tgagaggttg
     7141 caggcaatcc accagcacaa tctggcggtc aaagcgcaga aaatgattct tatagaaccc
     7201 cttcaccact ttctcgcagt aataattaaa ccgctcgcgc agcattccgg cattggtgtg
     7261 cttatcggcc tgcgccagtt tggactcgtc ccaggcatcg acatccggcc acgggaagaa
     7321 ttgcagcgcg ggcgcacctg ccatatctcc cggcaagaca aagcgcccag gctgaataaa
     7381 gtgcagcccc tgctgtttac agtggtggag ataatcggtc cacacggcgg caatgtctgc
     7441 cagacggttt tcgtcggcag gcgctagcgg gtcaagtcct tcgcacatca ttcgccattt
     7501 cgccgaccat tcgccgcgct gaccattgag taagcccgtc atctggcgcg accagcttaa
     7561 gtaatcctgc gccagcatcg gcaggtcgag caaccattcg ccgggataat ccacaatttc
     7621 cagatacagc gtggaggtat ccttaaagtg gcgcagcagc gcatcgttcg atttaaaacg
     7681 taacgccagg cgaatttcac tgacgccgcg cgtaggcgtc ggccaggcgg gaggatcacc
     7741 atataactgc gccagtcctt catcataggt gaagcgcgga atgccaaagt cacgctgggg
     7801 aatgcgtttt acgcccagca agcgctcttc acgcaccgcg cttaacagcg gcaaacgtgc
     7861 tccggcatga atattgagca actgattaac catcgcggtg ataaacgctg ttttgccgct
     7921 gcggctaagt ccggttacag cgaggcgcag atgtctgtcg acaccccgat tcaccagcgc
     7981 attgagttca tttttaagtc gcttcattgc tgtcctgtgc tgctctgaag accagaaaaa
     8041 aaatcattcg taaggatatg aatgtggggg ccaggccatt gattatcaac tgcccgtgtt
     8101 gtcatcgacg ggcgaactcc ataagatgtg cagcattcac gttcggacgg agttcctgac
     8161 ctatgtcccc tactatttat gatattgcca gggttgcagg cgtatcaaaa tccaccgtat
     8221 cacgcgtgct gaataagcaa actaatatct ccccggaagc gcgcgaaaaa gtgttacggg
     8281 ccattgaaga attacagtat cagccaaaca agctggcccg cgcgctgacc tcttcgggtt
     8341 ttgatgccat tatggtgatt tctacccgtt cgaccaaaac tacggcgggt aatccgtttt
     8401 tctctgaagt tttgcatgcc atcaccgcca aagcggaaga agaaggtttc gacgtgatat
     8461 tacaaacgtc gcacaatccg gcagaagatc tgcaaaaatg cgaaagcaaa attaaacaga
     8521 aaatgattaa aggcattatt atgctcagtt cgccagcgga tgagtcattt tttgcccaac
     8581 tcgataaata cgatattccg gtggtggtaa ttggcaaagt tgaaggtcaa tatgcccatg
     8641 tttattctgt cgataccgat aattttggtg acagcattgc gttgaccgat gcattaattg
     8701 aaagtgggca tcaaaatatt gcctgcctgc atgctccgct tgatgtccat gtttcggttg
     8761 atcgcgttaa tggctataag caaagtctgt ccgcgcataa tattgcagtg cgtgataaat
     8821 ggattgttga cggcggttat acccatgaaa cagcactgca agcagcacgg caattattaa
     8881 gtcagtcacc gttgcctgag gcagtgtttg ccactgacag cctgaaatta atgagcattt
     8941 atcgtgcggc ggcagagaaa aatattgcta ttccgcagca gttagcggtg gtgggttata
     9001 gtaatgaaac gctgtcgttt attttaacgc ctgcaccagg cggcatcgat gttccgacgc
     9061 aggagttagg tcaacaaagc tgcgagttat tattccgctt aatttcggga aaaccgtcac
     9121 cacagaatat taccgttgcc acgcatatga cgctgaaata aataccagct accttgcctg
     9181 aatgccggat gcgatgctgg cgcatcttat ccggcctaca tttatagatc ggataaagta
     9241 ctcgcgccgc atccggcaat ttagcccatt atcagaacga gtaatttacg ccgacacctg
     9301 cataatggaa tttatcgctg tcgccttcgt cgtgatcctg ccactcaaac gcgtattcca
     9361 gcgaaacgga aagtccgttc tggaaatcat aaccgtaaaa taaacccaca cggttaaaat
     9421 catggccttc acgttcaata tcatcctgcc agtcccagtt actctagcga tccagcccaa
     9481 tgcgcgtata cggcgtcacc gagtggttgc cgagcgtcag cggcaaatag gcgcgaattt
     9541 cttgtgtgga aaactcaccg ttattgcggc tgtcgtccat attgaagccg cgctcgagat
     9601 aatagttcac tcgcaaagca accgtttcgt tgaaggtata ttgcagacct gtttccgttt
     9661 cgacacgggt atcagcgtaa ccggtcgtgt tcaaatcgtt ggcaaattta tacatcgaca
     9721 accaaccgtt gaaacgtaaa tcatcagtca gtttcacatc ccagtctggc gcgattttcc
     9781 agcgctgcat attcgccgtg tctttgcccg gttcatcaac gtagtgataa ccataattac
     9841 ggaaaccgcc ggtcaggccg aaactgaaat catcgctttc gaggaactga taatgcacct
     9901 ccagctccgg gcgatcaaac ctcgttccac gtttacccgc actataatct accggccctt
     9961 cctgataata ggccagagca attctccacg gcccgttggc ggcattaaaa tagactgaag
    10021 gctccgccag cccatccata tcttcgccat aaccctcgac gttttctatt tcgtacatcg
    10081 caccgatatt aaagtgccag tcgctctttt cctcggcctg tgcgcaggcc attcccgcac
    10141 acatcaccag tgcggtacag ggtaataact ttttttgttc cttgtattat cctgggggaa
    10201 tgccttcccc ctgtatttag cgaattgcta tttccgtttc tgcatcaaag aaatgacatt
    10261 tcgtcatatc aaaatgaata gtgatatttt ctcctgcatg ataatcattt aacgcaccag
    10321 cacggaccac taactcgtgc ccccaaccgt ggtgtagagc ataaattccg caccggttaa
    10381 ttctgccacg ctaattttgg cggaaatgtt attttcctct tgcgcgtccg gatgaatatc
    10441 ttccgggcgt attcccatca cgatgggctt atgcaaactt tcctgtgttt tcagaaccgc
    10501 taatttctct tcgagaatgg ttaatttaag cgtttccgta acgaatttat cgccatcgat
    10561 cgtgccgcga ataaaattca tcgctggtga tccaataaat ccggcaacaa acatattcgc
    10621 gggttggtta taaacggttt tcggcgcacc tacttgctga acaatcccgt ctttcataat
    10681 cacaatccgc gccgccatgg tcatcgcttc ggtctgatcg tgggtcacgt agatcatggt
    10741 ggtgttcagt ttctgatgca gcttgctgat ctctgcacgc atttgtacgc gcagcttggc
    10801 atcaaggtta gagagcggtt catccattaa aaacacgccc gcttcgcgca caatcgcccg
    10861 cccgagcgcc acacgctggc gttgcccgcc ggaaagcgcg cccggcttgc gtttcaggta
    10921 ctcacgcagg ccgagaattt gcgccgccca gttcacccgc tcatcaatca cctctttggc
    10981 gattttttgc atcttcaggc caaacgccat gttgtcgtaa accgtcatat gcggatacaa
    11041 cgcgtagttc tggaacacca tcgcgatatt gcgtgctttg gctggaacgt cattcattcg
    11101 tttgccgtcg atcaacagat cgccgccgct gatctcctca agcccggcaa tcatgcgcag
    11161 ggtggtcgat ttaccgcagc ccgacgggcc gacaaatacg atgaactctt tatcggcaat
    11221 ttccaggttg aagtccttca ccacatgcac ctggttatcg tagatttttt gaatatgttg
    11281 taacgaaagc tgagccatgt tgattccttt gctatacgtt ttgccagaag gccgataacc
    11341 gcgaccaggt cagggagtcc gttgagggca acagtaattg cgccccggtt aagcccgcac
    11401 cgatccccac cgagcgcata ccgctggcgt taatggcgtc aatgcccgcc tgcgcatctt
    11461 caatgccgat acatgcctgc ggcggcacgc ccagcccggc acaggcagcg agaaagattt
    11521 ccggatccgg tttcgagttt ttaagttggg aagcatccgc gcagaaggtg aaaaactcac
    11581 gcagctccag cgccgctaaa atcgtcggcg cattcaggga gacggaagcc agccctaccg
    11641 gaatctgctg tgcacggagc tctgccagca aattgcgaat accgggcagc acagcattaa
    11701 ccgtcagctc gcgcagtgag tggacgtaaa gcagattttt gcgatacgcc agttgcgccc
    11761 gctcctgcgg gttaaagtcg ccctctttgc ccccgtgttg cagaatgcgc cgcagagact
    11821 catcgcggct gatccctttt agagattcgt taaactgcgc atcaatactg atgccaattt
    11881 cagcggcaat ctgctgccac gcctggaaat gcagatgcgc ggtatcggtg attacaccat
    11941 ccagatcgaa aattacccct tgcagtttca ttcatcctcc tgatgcgtgg tagcggtccc
    12001 attgaagggc aaaataaaat cacccaaaca gaaaacagat tcttcagcca cggatataag
    12061 ctgaccgtcc aaacgcagtg aaacgggcgc agaagttcga atcgcaatac gctgcgcgtc
    12121 gagagtgacc tgtaattcgc agccctgcca gaacagaggg aaagacaact gttgccactg
    12181 ctcaagtaac gccggattta gatgcaattc accgtcacgc acgctcaccc cggcaaaacc
    12241 ctgaatcgcc cccagccaga tagcgccagt tgcggcagca tggataccat catcacaact
    12301 atgcggatca gcaccaagat cgatttcagt cccctcgcgc cagaactgat aactttgggt
    12361 cagcaggcca cagcgtgcag caacaatgcc gtggattgct ttacttaatg acgagtcgtg
    12421 aatagtgcgc ggttcataaa attgcagatt ggcaagacac gatgccgatg agaactgctc
    12481 tggcagcatg taattgagca tcaccacatc agcttgtttg aggatctgca tctcgttcac
    12541 ttctgcgcgt gaataatcca gcagaatggt ttgcttcccc gccgccgctt tgtatttcgc
    12601 cagattaatc gccggcttag tcataaacga atcatcctgc ggcaaaacac cgtcgggctg
    12661 aatttctggc agccgcagtt ctttaaggaa catttcggca cgatgaataa acgcgtcgtc
    12721 actacagcca aactggcggg caatactcag cgcctgctgg acgttgtaat acaccatata
    12781 gctggtgaag gcgttattat tgacatgttc ggtatattcg tctggcccaa taacatcatg
    12841 aatttccaga cggtcgttaa ccctcaccgc gcggctaatc cagaactttg cagtttccag
    12901 aagtagcgcc atgccttcat gcgcaatgaa actttcatcc cccgtggtct gccagtattg
    12961 aataaccgcc caggcgatat cggccaccag atgatgttcc gcctgcgccg aggccacttt
    13021 ttgccgcagc ccggtgcgaa tgttaatggc ggcaaattcc ggcgtctctt cttcgccgct
    13081 gcgcgcgctt tcccacggaa atagcgcgcc ctgccagccg ttgcgtcgcg ctttctcctg
    13141 cgcgcctggc aagttgtgcc agcgataacg cagtaaactt cgggcaaccg tcggatcgct
    13201 aaacagatgg aacggcagca aaaatacttc tgtatcccag aaaacgtggc ctttgtagcc
    13261 ttcgccggtt aagccttttg ccgcaatact gctgcgctcg ccgtgagccg gcgtcatgat
    13321 gcgcagatga taaagcgcat aatctaacgc ttgctgatcg tgcgcatcgc cgccatttac
    13381 cgtgatacgg cgtttctgcc accattgacg ccagttttct gttgatgctg caagatgttg
    13441 gtcgtaactc tgttgcgcgc acatttcaag ctggcgaagc gacacgctgc cccactcgtc
    13501 taaagcagct tgcctgtcat cccgccagtc gatccacacc agtttttgca acgtcactgt
    13561 ctcgcctgca tgaagctgcg tactggtatg ttgcagtaaa cggcgctctt tggcggtata
    13621 gcattgctgc acatcaccgc tcacctggca gcaacaactg atggccacat cactgcgccc
    13681 atcctgggtg gtgtagatcc cctgcatcaa atgctgacca aacacccgca cctgggtttc
    13741 gtcgagatgc tggcgaccgt ggttggtttg cgtggcgtca atgcctgttg aaatcagcac
    13801 tgacgcgtcg gcgtccagtg gcataatagt gatttccagc gcaatgagcg gcagttggtc
    13861 tgccgaaaca aagcgacggc tggcgatggt gtaacctgcg ccgttgctgg tacgccagac
    13921 aacgctacgg cgtaattctc cactggcaaa gtcaagttca cgctgccagg cttcgcggga
    13981 taacgagaaa acctcaccat ttatggcaat ctccatcccc aagatatcag gcagattcac
    14041 cagttcgttg atttcacctt ttcccgcccg atgatacagc cccgccagat acatccctcg
    14101 cgtctggcgc gtgtaatctt cttcatggct ggcgcgaagc ccaagatagc cgttcccctg
    14161 cgccatcagc gatgcatact tgttcagggt atgctggctg aaatggggtt ctgataacgt
    14221 tactggcctg gtcataattc cacacgcgtc cctgtttctg cggattgata taacgccgcc
    14281 accagttgct ggatgatgta cccctgctcg gcgtcggcta tcatcacggg cttgccctgt
    14341 acgtgattga taaaggcttc catgctgcgc aaatggcggt tgtcgtctgc catttcccgt
    14401 tgcatcagcg tcattaattc accgttgtta tcggtgtaga tatgtgctgg aaacagcgtc
    14461 gcaccggctt tatcaccaca aaagctgacg ttcataatca actgttcgcg gatgttgagt
    14521 gcaaatgacg tttccagcca cagaatgccg ccgttatgaa attcaatggt gccaaacagc
    14581 gaatcttcga cgctgtaagt tgccggatcc cactcgccaa attgaccaca gctcttttgc
    14641 gtgccgatct tttgaaaact atgcgcattc acgcttttca ccgccggaaa gcccagcaca
    14701 tacatcgcag catccagcat atgaatgccg atgtcgatca gcaggccacc accctgcagt
    14761 tctttattgg taaagacacc ccagccggga acgccgcagc gacgcagggc gcgggcggtg
    14821 gtaacgtaaa tctctcccaa aacgccgttg gtcacctgtt cacgcagctg ttgcgtatcg
    14881 agcgcaaacg atggtgaaag tcgtaggcca gcaccttgcc cagtttgcgc gcggtatcgc
    14941 acatttccca cgcctgttct gtcgtcatgg cgggcggttt ttcgcacatc acatggcagc
    15001 ctgcttccag tgccatcagg gtatgttcgt aatgaaaacg gttaggtgag ccgacgctaa
    15061 ccacatcagg tttcaccgcc agcagcatgg cctgcggatc gtcccacacg gatgcattcc
    15121 cgtatttttc tgccagcgcc tgcgcctggg aaaggtggct gtcacagaca gccaccagtt
    15181 ccagatcgtt gcgggtgcag tagtacgaag catgaacctt atccgccacc tggcccgcgc
    15241 ctattatcgc gacccgcagc ggagagcttg tcattgcact tttcacaaag acctcttagc
    15301 aggtacgcaa ccaggccaac gaatcacggt acgcctgggc aggatcttct gcccggatac
    15361 gcccttcata caccacataa ccctgatagt tatcggcgcg cagctgttca aacagcgcgt
    15421 ggaaatcgag ggtgccactg cccggctggt aacgatggtt atcggcaata tgcacatgac
    15481 cgagcaagtc acggttgtcg tgcagcgcct gcgccaggtt atcttcttcg atgttcatgt
    15541 gatagaaatc gccgataatc tgcacatgtt taagattgtt ttcgacgatg taacgacgag
    15601 catcggcgag ggtgttgatc atatgatcct gatagcggtt taacggttcg agatacacca
    15661 cggttccggt acgcgcggcg acctgttcca gtacgcgcag ggaatcactc accagtttgc
    15721 ggtcgccgtc caggctacgc ggcgaggtca tcggcggtaa gcggaaggta aacatgcccc
    15781 acgctgccgg aaccccccca cttctgccag cgcctcgaga atgcgttcga tctgctttaa
    15841 gccattaaga cgacgctctt cgataaagtc accaatccag ccgtcatagc cgccgcaggc
    15901 ggtggtcact ggtaaaccgg tttctttgat cgccgctttg acttcttcga tgttgttgac
    15961 cagcagtttg ccgtcaatct caaaaccatc gaagcccatc tctttgatat aacgaaatct
    16021 ctcgcggata ttttccggaa agaatgcctg attctgtgtg ccgattttca ttttttcatt
    16081 cctttgaatt aaaacgtaac gcccattttg atgctctgtt ccggatgctg atcgacatac
    16141 tgcatgtagc tttccgggct gttggcaaag gtcactaccg ggtcgattaa atcttcgcaa
    16201 ttgagataac cgttcatcag cagttcccag caggtttctt caatacgctt acggctccag
    16261 cgcggataat ccgggttcgg ttcgctgcac gcgcgggaga aaacaatttt ggcgttattg
    16321 aaatgcgctt cgcgtccgag gttaaaacct tcagcaaatg gtttggcaaa cgcgacatag
    16381 gagatggtgc cgccgtaggc caggccgcgc agcgccgatt gcagcgcgtc ggcgtaaccg
    16441 ctggtttcga taatcacatc agccccctgc ttgccggtca gcgttttgat ctctttgcct
    16501 acgtcagtgc caatggggtt aaggcagaaa tcagcaccgt ggcgacgggc aatatcacag
    16561 cgatgggcga taggatcgac gccaattacc accgatgcgc cagcgcgttt cgccagttgg
    16621 atggcaattt gaccgatcgc gccaagccct accaccacga caaaatcccc tacgcgtacg
    16681 ttggcatcgc gcacgccgct catggcaaac tgcgccgggt cgtaacagac ggcgtttttc
    16741 caggagctgc cttgcggcat tttgcgcagc ttgtagttat tcactgcgtt aatgatgacc
    16801 gtctcggaga gcgggccgta gccgcatacg ctgtcgccca ccgcgtagtc tgtaacgtcg
    16861 ctgccgcact cgataatgtc gccaaccacc atgttgccaa gctggaattt gccaaactca
    16921 atgccgcgcg gcgcatctgc cggacgcggg gtgaacatct gccactcgcc gttaaagtct
    16981 tcatcgataa acgggctggc ggcgcggaag tcgaccactt ccgttccgtg tttcggtgcg
    17041 ccgaaacgca cgcggatctt cacttcattg gctaaaatcg cccgatcttc atactcaacc
    17101 agcgcagcaa cacgcggtgc tgtggctact aactttttca tgtaaaactc cttgtaaaga
    17161 aactggccgc tttagccctt cacgccaccg gcggtcaaac cactcttaat aaaacgttcc
    17221 aaaagcgcat acataatcac caccggcaat gcggtcacca gcgaggccgc catcatccgt
    17281 ccccagatgt aatcaggcgt actgaacagc gcgttcaggc ccactggtaa ggtgaaattg
    17341 ctggcgctgg agaggaaaat cgacgcaaac agatagtcgt tccacgccac cataaagcag
    17401 tagacgaaca ccgatatcag cccggacatc gccaggggca cggtgatacg gaagatgatt
    17461 tgcagacggt tgaggccatc catcatcgcc gcttcttcga tctcatcagg gatggtgtcg
    17521 aagtaacttt tcagcataaa cacggcggtg ggcagggttt gcgtcaccat ggtaatgatc
    17581 agcgccatct cggtgtcgta aataccaagc gcggtgatga ttttgaacag aggcaccacc
    17641 agcaaaatgc cagagaacat atacaccgtg taaaagctgg cgttgatggt catccggcct
    17701 ttaaagcgca ggcgggaaag cgcgtaagct ccgagaatgc cgagaaatac cgccaccacg
    17761 gaggaaacca ccgacaccac catgctgtta cggaagtagt cgacaaacgg gaaaatcatc
    17821 gggttaaaaa tgtcgacgta atgctccagc gtccactgtt gcggcagcag cgtaggatgc
    17881 agcgatatcg cctctttcgc gcttttgaac gaggtcatca gcatcacaaa aaacggaaac
    17941 agcgtgatga tgagaaacag cgccagcccg cagtaaaaac cgatacgact gagcgtgcgt
    18001 ttatttgttg ccattgaggt tcacccgttt cctggtcagc agaatgacag cgaaaataat
    18061 gatgaagagc accaccgaga tcgccgccgc tttccctaag tcattaaagg caaaggcggt
    18121 tttgtagaga tagacaccaa gaatatcgac cttggttgtc agcagataaa catcagcgaa
    18181 catgtagaac atccagatgg tgcgcagggt caccaccgtc gccaggaccg gcataattgc
    18241 gggcagcgtg acgatgcgaa accgctgcca ggcattagcg ccatccatct ctgccgcttc
    18301 gtacagcgat ttgtcgatgg tctgcaaaat cgccagaaac gagataaagg catacgggaa
    18361 gtagcgccag atggcgaaca gcaccaccag cacaaagcta ctgcccggat tatcaaacca
    18421 cagcggtgcc tgctcataga gatgcagaag atcgacgccg aggtagttca caatgccgta
    18481 gccgttgttg aacatgtatt tccaggcaaa caccagcgaa atggacggcg ttacgtagga
    18541 gaggatcacc agcgaacgcg cggttttgcg caggcggaac tcgcggttga aaaacatcgc
    18601 cacggccagc ccgagcacgg tgctgcccgc caccaccagc gccgtatacc agaccgtcat
    18661 ccacagcgag tgccagaagc cgggatcgga gagaatgcgc acatagttac tcaccccaac
    18721 aaacgttgac tcgatgttgg gattgagcgg cagacgtaaa aaactgattt cgatattcga
    18781 caccatcggc cacgccacca gaccgcccag cagtaataag ctgggcgcga gaagcagcag
    18841 cgcaaacggc atatcggaac gacctgaaaa aagcctgttc attgcggatt cctgtcctgg
    18901 gtttagtgct gttcgatcag ttcatccagc tttttctggc tcgcttgcag cgtgccaggg
    18961 agatcggctt tacccacggt gacgttatgc accatggagc tcaccacacc agaacccgtc
    19021 acatcgccca tgcgggtaaa gtttttatcc cctactgcgc caaagacctg aatatttggc
    19081 agttcaccga ttagctgatt cggtagttca cccagcgcct taataacgtc gttgtctttc
    19141 caggtggcgg tagtgacaac cgctttattc accggcagcg cagcacctgg cgacatcatc
    19201 acccagtcgg caatgttgtc cgcctgctcc ataaaggtga caaatttttc tgctgcttcg
    19261 gtcttttgtc cggcggtaat ggtcagcgag gtcaacatgc cgtagaccgc agagtttttc
    19321 tcggttggca ccacgaaacc gacgtttttc gggtcgcctt ctttaatcac agccggaagg
    19381 atataggtgg agtaaatcgc catcggcgcg gtgccgttca taaaggcgtc tttcacttcc
    19441 atgatgtcgt tagaacccgg catggtgttg gcagcaaggt cgcggtaata ggtcagcgcc
    19501 tgcatcatct ctagtgtatc aagggtgatt ttgccttcgg cgttaaagac gttggcctag
    19561 ttggataacg caaactggga gaaggattgt tccgtcagca cgctttctgc cgtaggcagc
    19621 gcaataccgt attttttatt cgccgggtca ttcagtttct gtgcaacgtc cagcagctgt
    19681 tgccagtttt tcggctcctc cagccccgct tttgccagca catctttgcg ataccagatg
    19741 ccgccaatcc aggcgctgac aggaacaccg gtccatgcgc taccatcttc ggtacgcaca
    19801 atacgcagta cgccatcgta aaacgcgcct tcaccaacgt tgctgatgac cgtggcaacc
    19861 gctttgcgat cgataagctg ctctttatcc ataactttgg cgtagtcatg gctggtttcg
    19921 atcacttccg gcagcgagcc gctacgtgaa agggtgatga ctttggtgtt atgagcgtct
    19981 tcttccacgg gcacttgttt aacggtgata cctgggtttt ctttttcaaa acgggcaatc
    20041 aatttgctga taactgcctg acgctcctgc tccaccgaag aatgcataaa ttcgatggat
    20101 acatgggttt tattttcttc tttacagcct gaaatcaggg cgcaggaaac cagtgctgat
    20161 aacagcacga tttttgattt aatcatgaga tgttcctttt tatttaatcc acataacctg
    20221 ccaggggcgc agcgttattt cattacccaa tatatcaact tcactaatca gatcccgacc
    20281 atgcagatca gtaatattta catgctgaat atttttactg acattaaaca atccagtcag
    20341 acaattacca tcagcgttac ttctttgaat acgcattacg gatgaattaa ccgtatcgat
    20401 ggtaaaatta ttatccggat gaaactcgtt atggctgcga cgaagtgtaa ttaaacgcga
    20461 caactcatga tataccgcat gccttaatgt agcttcatcg ttcagttctc gtgttatctc
    20521 ttcactgtga tattttttac ggttaatcgc acggttatat ccgagttttt cgacacctgc
    20581 gtaatcatta cgcgagccaa gaatactttg aatatatatc gcgggtacac cggggaaact
    20641 taacaaaatc gcatgggcaa ggataaacct ggtgcaacgt tcttcatcgc tactctcgcg
    20701 gcggcttaac gcattcatat aggtgacatt catctcatag ggactgcgcg tgccgtcggg
    20761 attatttttc cagtttact
//
LOCUS       DAJTRC010000067        20692 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.067, whole genome shotgun sequence.
ACCESSION   DAJTRC010000067 DAJTRC010000000
VERSION     DAJTRC010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20692)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20692)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20692
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.067"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            82..351
                     /locus_tag="OO357_002668"
     CDS             82..351
                     /locus_tag="OO357_002668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HCR5887567.1"
                     /translation="MQTVKLRQQGGAMIVTIPRDLAIDLGWSPGTELTVEKKGDSVNL
                     RATEHKPRGRLTVAQLLSQIDESEITELNQSTEGWAEGKKGNEAW"
     gene            345..710
                     /locus_tag="OO357_002669"
     CDS             345..710
                     /locus_tag="OO357_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HCR5887568.1"
                     /translation="MVKARTPHRGEIWYFNPDPVAGHELQGPHYCIVVTDKKLNNVLK
                     VAMCCPISTGANAARSTGVTVNVLPRDTQTGNLHGVVLCHQLKAVDLIARGAKFHTVA
                     DEKLISEVISKLVNLIDPQ"
     gene            complement(711..1499)
                     /gene="bioH"
                     /locus_tag="OO357_002670"
     CDS             complement(711..1499)
                     /gene="bioH"
                     /locus_tag="OO357_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709189.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-ACP methyl ester esterase BioH"
                     /protein_id="HCR5887569.1"
                     /translation="MNNIWWQTKGQGNVHLVLLHGWGLNAEVWRCIDEELSSHFTLHL
                     VDLPGFGRSRGFGALSLADMAEAVLQQAPDKAIWLGWSLGGLVASQIALTHPERVQAL
                     VTVASSPCFSARDEWPGIKPDVLAGFQQQLSDDFQRTVERFLALQTMGTETARQDARA
                     LKKTVLALPMPEVDVLNGGLEILKTVDLRQPLQNVSMPFLRLYGYLDGLVPRKVVPML
                     DKLWPHSESYIFAKAAHAPFISHPVEFCHLLVALKQRVLVVSES"
     gene            1537..2220
                     /gene="gntX"
                     /locus_tag="OO357_002671"
     CDS             1537..2220
                     /gene="gntX"
                     /locus_tag="OO357_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417872.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA utilization protein GntX"
                     /protein_id="HCR5887570.1"
                     /translation="MLTVPGLCWLCRMPLALGHWGICSVCSRATRTDKTLCPQCGLPA
                     THSHLPCGRCLQKPPPWQRLVTVADYAPPLSPLIHQLKFSRRSEIASALSRLLLLEVL
                     HARRTTGLQLPDRIISVPLWQRRHWRRGFNQSDLLCQPLSRWLHCQWDSEAVTRTRAT
                     ATQHFLSARLRKRNLKNAFRLELPVQGRHMVIVDDVVTTGSTVAEIAQLLLRNGAATV
                     QVWCLCRTL"
     gene            2279..2854
                     /gene="nfuA"
                     /locus_tag="OO357_002672"
     CDS             2279..2854
                     /gene="nfuA"
                     /locus_tag="OO357_002672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016942932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S biogenesis protein NfuA"
                     /protein_id="HCR5887571.1"
                     /translation="MIRISDAAQAHFAKLLANQEEGTQIRVFVINPGTPNAECGVSYC
                     PPDAVEATDTALKFDLLTAYVDELSAPYLEDAEIDFVTDQLGSQLTLKAPNAKMRKVA
                     DDAPLMERVEYMLQSQINPQLAGHGGRVSLMEITEDGYAILQFGGGCNGCSMVDVTLK
                     EGIEKQLLNEFPELKGVRDLTEHQRGEHSYY"
     gene            3215..4531
                     /gene="gntT"
                     /locus_tag="OO357_002673"
     CDS             3215..4531
                     /gene="gntT"
                     /locus_tag="OO357_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate transporter"
                     /protein_id="HCR5887572.1"
                     /translation="MPLVIVAIGVILLLLLMIRFKMNGFIALVLVALAVGLMQGMPLD
                     KVIGSIKAGVGGTLGSLALIMGFGAMLGKMLADCGGAQRIATTLIAKFGKKHIQWAVV
                     LTGFTVGFALFYEVGFVLMLPLVFTIAASANIPLLYVGVPMAAALSVTHGFLPPHPGP
                     TAIATIFNADMGKTLLYGTILAIPTVILAGPVYARVLKGIDKPIPEGLYSAKTFSEEE
                     MPSFGVSVWTSLVPVVLMAMRAIAEMILPKGHAFLPVAEFLGDPVMATLIAVLIAMFT
                     FGLNRGRSMDQINDTLVSSIKIIAMMLLIIGGGGAFKQVLVDSGVDKYIASMMHETNI
                     SPLLMAWSIAAVLRIALGSATVAAITAGGIAAPLIATTGVSPELMVIAVGSGSVIFSH
                     VNDPGFWLFKEYFNLTIGETIKSWSMLETIISVCGLVGCLLLNMVI"
     gene            complement(4576..6660)
                     /gene="malQ"
                     /locus_tag="OO357_002674"
     CDS             complement(4576..6660)
                     /gene="malQ"
                     /locus_tag="OO357_002674"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HCR5887573.1"
                     /translation="MESKRLDNAALAAGISPNYINAHGKPQSISAETKRRLLDAMHQR
                     TATKVAVTPVPNVMVYTSGKKMPMVVEGSGEYSWQLTTEEGTQYKGHVTGGKAFNLPT
                     KLPEGYHTLTLTQDDQRAHCRVIVAPKRCYEPQALLNKQKLWGACVQLYTLRSEKNWG
                     IGDFGDLKAMLVDVAKRGGSFIGLNPIHALYPANPESASPYSPSSRRWLNVIYIDVNA
                     VEDFHLSEEAQAWWQLPTTQQTLQQARDADWVDYSTVTALKMTALRMAWKGFAQRDDE
                     QMAAFRQFVAEQGDSLFWQAAFDALHAQQVKEDEMRWGWPAWPEMYKNVDSPEVRQFC
                     EEHRDDVDFYLWLQWLAYSQFAACWEISQGYEMPIGLYRDLAVGVAEGGAETWCDREL
                     YCLKASVGAPPDILGPLGQNWGLPPMDPHIITARAYEPFIELLRANMQNCGALRIDHV
                     MSMLRLWWIPYGETADQGAYVHYPVDDLLSILALESKRHRCMVIGEDLGTVPVEIVGK
                     LRSSGVYSYKVLYFENDHEKTFRAPKAYPEQSMAVAATHDLPTLRGYWESGDLTLGKT
                     LGLYPDEVVLRGLYQDRELAKQGLLDALHKYGCLPKRAGHKASLMSMTPTLNRGLQRY
                     IADSNSALLGLQPEDWLDMAEPVNIPGTSYQYKNWRRKLSATLESMFADDGVNKLLKD
                     LDRRRRAAAKKK"
     gene            complement(6670..9063)
                     /gene="malP"
                     /locus_tag="OO357_002675"
     CDS             complement(6670..9063)
                     /gene="malP"
                     /locus_tag="OO357_002675"
                     /EC_number="2.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrin phosphorylase"
                     /protein_id="HCR5887574.1"
                     /translation="MSQPIFNDKQFQEVLSRQWQRYGLNSAAEMTPRQWWLAVSEALA
                     EMLRAQPFAKPVANQRHVNYISMEFLIGRLTGNNLLNLGWYQDVQDSLKAYDINLTDL
                     LEEEIDPALGNGGLGRLAACFLDSMATVGQSATGYGLNYQYGLFRQSFVDGKQVEAPD
                     DWHRSNYPWFRHNEALDVQVGIGGKVMKDGRWEPEFTITGQAWDLPVVGYRNGVAQPL
                     RLWQATHAHPFDLTKFNDGDFLRAEQQGINAEKLTKVLYPNDNHTAGKKLRLMQQYFQ
                     CACSVADILRRHHLAGRKLHELADYEVIQLNDTHPTIAIPELLRVLTDEHQMSWDDAW
                     AITSKTFAYTNHTLMPEALERWDVKLVKGLLPRHMQIINEINTRFKTLVEKTWPGDEK
                     VWAKLAVVHDKQVHMANLCVVGGFAVNGVAALHSDLVVKDLFPEYHQLWPNKFHNVTN
                     GITPRRWIKQCNPALAALLDKSLQKEWANDLDQLINLEKFADDAKFRQQYREIKQANK
                     VCLAEFVKVRTGIEINPQAIFDIQIKRLHEYKRQHLNLLHILALYKEIRENLQADRVP
                     RVFLFGAKAAPGYYLAKNIIFAINKVADVINNDPLVGDKLKVVFLPDYCVSAAEKLIP
                     AADISEQISTAGKEASGTGNMKLALNGALTVGTLDGANVEIAEKVGEENIFIFGHTVE
                     QVKAILAKGYDPVKWRKKDKVLDAVLKELESGKYSDGDKHAFDQMLHSIGKQGGDPYL
                     VMADFAAYVEAQKQVDVLYRDQEAWTRAAILNTARCGMFSSDRSIRDYQARIWQAKR"
     gene            9675..12380
                     /gene="malT"
                     /locus_tag="OO357_002676"
                     /pseudo
     CDS             9675..12380
                     /gene="malT"
                     /locus_tag="OO357_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312287.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator MalT"
     gene            complement(12423..13439)
                     /gene="rtcA"
                     /locus_tag="OO357_002677"
     CDS             complement(12423..13439)
                     /gene="rtcA"
                     /locus_tag="OO357_002677"
                     /EC_number="6.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 3'-terminal phosphate cyclase"
                     /protein_id="HCR5887575.1"
                     /translation="MKRMIALDGAQGEGGGQILRSALSLSMITGQPFTITGIRAGRAK
                     PGLLRQHLTAVKAAAEICRATVEGAELGSQRLVFRPGTVRGGDYRFAIGSAGSCTLVL
                     QTVLPALWFADGPSRVEVSGGTDNPSAPPADFIRRVLEPLLAKIGIHQQTTLLRHGFY
                     PAGGGVVATEVSPVASFSTLQLGERGNIVQMRGEVLLAGVPRHVAEREIATLAGIFSL
                     HEQNIHNLPRDQGPGNTVSLEVESENITERFFVVGEKRVSAEVVAAQLVKEVKRYLAS
                     PAAVGEYLADQLVLPMALAGAGEFTVAHPSCHLLTNIAVVERFLPVRFGLIETDGVTR
                     VSIE"
     gene            complement(13443..14669)
                     /gene="rtcB"
                     /locus_tag="OO357_002678"
     CDS             complement(13443..14669)
                     /gene="rtcB"
                     /locus_tag="OO357_002678"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-splicing ligase RtcB"
                     /protein_id="HCR5887576.1"
                     /translation="MNYELLTTENAPVKMWTKGVPVEADARQQLINTAKMPFIFKHIA
                     VMPDVHLGKGSTIGSVIPTKGAIIPAAVGVDIGCGMNALRTALTAADLPENLAELRQA
                     IETAVPHGRTTGRCKRDKGAWENPPFNVDAKWAELEAGYQWLTQKYPRFLNTNNYKHL
                     GTLGTGNHFIEICLDESEQVWIMLHSGSRGIGNAIGTYFIDLAQKEMQDQLETLPSRD
                     LAYFMEGTEYFDDYLRAVAWAQLFASLNRDAMMENVVTALQSITQKTVRQPQTLAMEE
                     INCHHNYVQKEQLFGEEIYVTRKGAVSARAGQYGIIPGSMGAKSFIVRGLGNEESFCS
                     CSHGAGRVMSRTKAKKLFSVEDQIRATAHVECRKDAEVIDEIPMAYKDIDAVMAAQSD
                     LVEVIYTLRQVVCVKG"
     gene            14850..16448
                     /gene="rtcR"
                     /locus_tag="OO357_002679"
     CDS             14850..16448
                     /gene="rtcR"
                     /locus_tag="OO357_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator RtcR"
                     /protein_id="HCR5887577.1"
                     /translation="MRKTVAFGFVGTVLDYAGRGSQRWSKWRPSLCIYQQESLVIDRL
                     ELLHDARSRSLFETLKRDIASVSPETEVVGVEIELHNPWDFEEVYACLHDFARGYEFQ
                     PEKEDYLIHITTGTHVAQICWFLLAEARYLPARLIQSSTPRKKEQPRGPGEVTIIDLD
                     LSRYNAIASRFAEERQQTLDFLKSGIATRNPHFNRMIEQIEKVAIKSRAPILLNGPTG
                     AGKSFLARRIFELKQARHQFSGAFVEVNCATLRGDTAMSTLFGHVKGAFTGARESREG
                     LLRSANGGMLFLDEIGELGADEQAMLLKAIEEKTFYPFGSDRQVSSDFQLIAGTVRDL
                     RQLVAEGKFREDLYARINLWTFTLPGLRQRQEDIEPNLDYEVERHATLTGDSVRFNTE
                     ARRAWLAFATSPQATWRGNFRELSASVTRMATFATSGRITLDVVEDEINRLRYNWQES
                     RPSALTALLGAEAENIDLFDRMQLEHVIAICRQAKSLSAAGRQLFDVSRQGKASVNDA
                     DRLRKYLARFGLTWEAVQDQHSSS"
     gene            complement(16430..17188)
                     /locus_tag="OO357_002680"
     CDS             complement(16430..17188)
                     /locus_tag="OO357_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000815090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family transcriptional regulator"
                     /protein_id="HCR5887578.1"
                     /translation="MKQTQRHNGIIELVKQQGYVSTEELVEHFSVSPQTIRRDLNELA
                     EQNLILRHHGGAALPSSSVNTPWHDRKATQTEEKERIARKVAEQIPNGSTLFIDIGTT
                     PEAVAHALLNHSNLRIVTNNLNVANTLMVKEDFRIILAGGELRSRDGGIIGEATLDFI
                     SQFRLDFGILGISGIDSDGSLLEFDYHEVRTKRAIIENSRHVMLVVDHSKFGRNAMVN
                     MGSISMVDAVYTDAPPPVSVMQVLTDHHIQLELC"
     gene            complement(17205..18035)
                     /gene="glpG"
                     /locus_tag="OO357_002681"
     CDS             complement(17205..18035)
                     /gene="glpG"
                     /locus_tag="OO357_002681"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease
                     GlpG"
                     /protein_id="HCR5887579.1"
                     /translation="MLMITSFANPRVAQAFVDYMATQGVILTIQQHNQSDVWLADESQ
                     AERVRAELARFLENPADPRYLAASWLAGHTGSGLHYRRYPFFAALRERAGPVTWVMMI
                     ACVVVFIAMQILGDQEVMLWLAWPFDPTLKFEFWRYFTHALMHFSLMHILFNLLWWWY
                     LGGAVEKRLGSGKLIVITLISALLSGYMQQKFSGPWFGGLSGVVYALMGYVWLRGERD
                     PQSGIYLQRGLIIFALIWIVAGWFDLFGMSMANGAHIAGLAVGLAMAFVDSLNARKRK
                     "
     gene            complement(18080..18406)
                     /gene="glpE"
                     /locus_tag="OO357_002682"
     CDS             complement(18080..18406)
                     /gene="glpE"
                     /locus_tag="OO357_002682"
                     /EC_number="2.8.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase GlpE"
                     /protein_id="HCR5887580.1"
                     /translation="MDQFECINVADAHQKLQEKEAVLVDIRDPQSFAMGHAVQAFHLT
                     NDTLGAFMRDNDFDTPVMVMCYHGNSSKGAAQYLLQQGYDVVYSIDGGFEAWQRQFPA
                     EVAYGA"
     gene            18596..20101
                     /gene="glpD"
                     /locus_tag="OO357_002683"
     CDS             18596..20101
                     /gene="glpD"
                     /locus_tag="OO357_002683"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HCR5887581.1"
                     /translation="METKDLIVIGGGINGAGIAADAAGRGLSVLMLEAQDLACATSSA
                     SSKLIHGGLRYLEHYEFRLVSEALAEREVLLKMAPHIAFPMRFRLPHRPHLRPAWMIR
                     IGLFMYDHLGKRTSLPGSTGLRFGANSVLKPEIKRGFEYSDCWVDDARLVLANAQMVV
                     RKGGEVLTRTRATSARRENGLWIVEAEDIDTGKKYSWQARGLVNATGPWVKQFFDEGM
                     HLPSPYGIRLIKGSHIVVPRVHTQKQAYILQNEDKRIVFVIPWMDEFSIIGTTDVEYK
                     GDPKAVKIEESEINYLLKVYNAHFKKQLSRDDIVWTYSGVRPLCDDESDSPQAITRDY
                     TLDIHDENGKAPLLSVFGGKLTTYRKLAEHALEKLTPYYQGIGPAWTKESVLPGGAIE
                     GDRDDYAARLRRRYPFLTESLARHYTRTYGSNSELLLGNAGTVSDLGEDFGHEFYEAE
                     LKYLVDHEWVRRTDDALWRRTKQGMWLNADQQSRVSQWLVEYTQQKLSLAS"
     gene            complement(20370..20600)
                     /locus_tag="OO357_002684"
                     /pseudo
     CDS             complement(20370..20600)
                     /locus_tag="OO357_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001443257.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein"
BASE COUNT         4890 a         5693 c         5217 g         4892 t
ORIGIN      
        1 agtcagtaga tactctaccc tgcttaagtg ctactttgtg ctatcttgca tgtaacacaa
       61 agtagcactt agggaactga tatgcagaca gtgaaattac ggcaacaagg aggagccatg
      121 atcgttacta tcccacgaga tcttgccatc gatcttggct ggtcaccggg cacagagctt
      181 accgtggaga aaaaaggcga tagcgttaat ttgcgcgcca cagagcacaa accgcgtggg
      241 cgtcttaccg tcgcgcagtt gttgagccaa atcgatgaaa gcgaaattac ggagctaaat
      301 cagtccacag aaggatgggc tgaagggaaa aaaggtaatg aagcatggta aaggcacgga
      361 cgccacatcg tggtgagatc tggtatttta accctgatcc ggttgccggg catgaacttc
      421 aggggccaca ttattgcatt gtggtaacgg acaaaaaact caacaatgtt ttaaaagttg
      481 ctatgtgctg cccgatttca acaggggcaa atgcagcacg ttccacaggg gtgacggtga
      541 acgtcctccc ccgtgatacg caaaccggta acctgcatgg cgttgtactt tgtcaccagc
      601 taaaagccgt cgatcttatt gcccgtggcg ctaaatttca taccgttgcc gatgaaaaat
      661 tgattagtga agttatcagt aaactggtga atttaatcga cccacaataa ttagctctca
      721 cttaccacca acaccctctg cttcaacgcc accagcaggt gacaaaactc gaccggatgc
      781 gaaataaacg gcgcatgggc cgctttggcg aagatatatg attcgctgtg aggccaaagt
      841 ttatccagca tcggcaccac tttgcgcggc accagaccgt cgagatagcc atacaatcgc
      901 aaaaacggca tggacacgtt ttgcagcggc tgacggagat cgaccgtttt caggatttcc
      961 agcccgccat taagcacgtc aacctccggc atcggtaacg ccagaacggt tttcttcaac
     1021 gcccgcgcat cctggcgcgc cgtttcagtc cccatggttt gtaacgccag gaaccgctcc
     1081 actgtacgct gaaaatcatc actgagttgc tgctgaaatc ccgccagcac gtccggtttt
     1141 atccccggcc actcgtcacg agcactaaaa caaggtgacg acgccacggt gaccagcgcc
     1201 tgaacacgct cgggatgggt taacgcaatc tggcttgcca ccagcccgcc cagactccag
     1261 cctaaccaaa tggctttatc aggtgcctgt tgcagcacgg cttcggccat atcagcaagt
     1321 gacagcgcac caaatccccg gctacgcccg aagccgggca ggtcaacaag gtgcagcgta
     1381 aaatgcgagc taagttcctc gtcaatgcaa cgccacactt cggcattcag tccccatccg
     1441 tgcagcagca caagatgaac attcccctga cctttggtct gccaccagat gttattcatc
     1501 cgctattgtt ctcttttgac ttacaaggat gaacatatgc taacagtacc gggattatgc
     1561 tggctatgcc gaatgccact ggcgttaggt cattggggga tttgttcggt ctgttcacgc
     1621 gccacccgca cagataaaac gttatgccca caatgtggat tacccgccac acactcccat
     1681 cttccctgcg gtcgctgcct gcaaaaaccg ccgccctggc aaagactggt cacggttgcc
     1741 gactatgcgc cgccgttaag tccgcttatc caccagctta aattttcccg gcgcagtgaa
     1801 atcgccagcg ccctgtcacg tctgttacta ttggaagtct tacacgctcg tcgcaccacc
     1861 gggttgcaat tgccggatcg catcatcagc gttccgttat ggcagcggcg tcactggcgt
     1921 cggggattta atcagagcga tttgctgtgt cagccgttat cacgctggtt gcactgccaa
     1981 tgggatagcg aagccgtcac acgtacacgg gccactgcga cccagcattt tctcagtgcc
     2041 cggctgcgca agcgcaacct gaaaaatgcc tttcgtcttg aattgcccgt gcaaggtcgc
     2101 catatggtga ttgtggatga tgtcgttacc accggaagta ccgtcgcaga gattgcgcag
     2161 ttgcttttac gcaatggtgc ggcgactgtc caggtctggt gcctttgtcg aaccttgtag
     2221 agcctcgatg atgggcgtat tataaccaac taaaatagtc aactattagg ccattactat
     2281 gatccgtatt tccgatgctg cacaagcgca ctttgccaaa ctgctggcaa atcaggaaga
     2341 agggacacaa atccgcgtat ttgtgattaa ccctggcacg cctaacgctg aatgtggtgt
     2401 ttcttattgt ccgccggatg ccgtggaagc caccgacact gccctgaaat ttgacctgct
     2461 gaccgcgtat gttgatgagt taagcgcacc atacctggaa gatgcagaga tcgactttgt
     2521 taccgaccag ttgggttctc agttaacgct gaaagccccg aacgccaaaa tgcgtaaagt
     2581 ggcagacgat gcaccgctga tggagcgcgt ggagtatatg ctgcagtcgc agatcaaccc
     2641 acagcttgct ggtcacggtg gtcgcgtttc gctgatggaa atcaccgaag acggttacgc
     2701 cattctgcaa tttggcggcg gctgtaacgg ttgttccatg gtcgatgtga cgctgaaaga
     2761 agggatcgag aagcagctgc tgaacgaatt cccggagctg aaaggtgtgc gcgatctcac
     2821 cgaacaccag cgcggcgaac actcctacta ctaagttatc ccctcttttg tagattgccc
     2881 gatgcgacgc taaagcgtca tttcgggcct acgttgaaca tgtgccagac gttggcacag
     2941 cattgctttg gcaaatccct cccttcgttt ccccgcgata atatgaccaa cctctcataa
     3001 tttaaattta ccccgctctg gtgattctca aacgccaaat gttacccgta tcattcacat
     3061 gggtaccaaa catactcctg acatctgact acaataatta gttttagtgg gtatcagtcg
     3121 tggtgccgca atatctctgt tcccgattgg gataattaga gtttgtcgtc agaaaattga
     3181 cgttacccat aacaaatgaa aggccaggta aatcatgcca ttagtcattg ttgctatcgg
     3241 tgtaatcttg ttgttgctcc tgatgatccg cttcaaaatg aacggcttca tcgctctcgt
     3301 cctcgtggcg cttgctgttg gattaatgca aggaatgccg ctggataaag ttattggctc
     3361 catcaaagcc ggtgtcggcg ggacgctcgg tagccttgcc ctgatcatgg gttttggcgc
     3421 aatgctgggc aaaatgctgg cagactgcgg tggcgcacaa cgtatcgcca ccacgctgat
     3481 tgccaaattt ggtaaaaagc acatccagtg ggcggtggtg ttaaccggtt ttaccgttgg
     3541 ttttgccctg ttctatgaag tgggcttcgt gctgatgctg ccgctggtgt ttaccatcgc
     3601 ggcttccgcg aatattccat tgctgtatgt tggtgtacca atggcggctg cactgtctgt
     3661 gacccacggc ttcctgccac cgcatccggg cccgactgcg attgccacca ttttcaatgc
     3721 cgatatgggt aaaaccctgc tgtacggtac tattctggca atcccgaccg tgattctcgc
     3781 cggtccggtt tacgctcgcg tgttgaaagg tatcgataag ccaatcccgg aaggtctcta
     3841 cagcgcgaaa accttcagcg aagaagagat gccgagcttt ggcgtcagcg tctggacctc
     3901 tctggtgccg gttgtgctga tggcgatgcg tgcgattgcc gaaatgatcc tgccgaaagg
     3961 tcacgctttc ctgccggtag cggagttcct cggtgacccg gtaatggcaa cgctgattgc
     4021 cgtgctgatt gcgatgttca cctttggtct gaaccgtggt cgttcaatgg atcagattaa
     4081 cgacacgctg gtttcttcca tcaaaatcat tgcgatgatg ctgttgatca tcggtggtgg
     4141 cggtgcgttc aagcaggtgc tggtagacag cggcgtggac aaatacattg cttccatgat
     4201 gcacgaaacc aacatttctc cgctgctgat ggcctggtcg attgctgccg tactgcgtat
     4261 cgcgctgggt tccgcaaccg ttgcggcaat cactgcgggt ggtatcgcgg caccgctgat
     4321 tgcaacgacg ggtgttagcc cagagctgat ggttattgcg gtgggttccg gtagtgtgat
     4381 tttctctcat gtgaacgatc cgggcttctg gctgttcaaa gagtacttta acctgactat
     4441 cggcgagacc atcaaatcct ggtcgatgct ggaaaccatt atctcggtgt gcggtctggt
     4501 aggctgtctg ctgctgaata tggtgatttg ataacactga ctgccggatg catcgcatcc
     4561 gacatcaacc gcactctact tcttcttcgc tgcagctctg cgccgtctgt ccaaatcctt
     4621 cagcaacttg ttcacgccat catcggcaaa catcgactca agcgttgcgg aaagcttgcg
     4681 tcgccagttt ttatactggt aactggtgcc aggaatattc accggttcgg ccatatccag
     4741 ccagtcttcc ggctgtagtc ctaacagagc actgttactg tcggcaatgt agcgctgcaa
     4801 accacggttc agcgtcggcg tcatcgacat caacgatgcc ttatgcccgg cacgttttgg
     4861 cagacaacca tatttatgca gtgcatccag cagcccttgc ttcgccagtt cgcgatcctg
     4921 atacagaccg cgcagtacca cttcatccgg atacagcccc agggttttgc ccagcgttag
     4981 atccccgctc tcccagtaac cacgcagcgt tggcaggtca tgtgtcgccg caaccgccat
     5041 cgactgctcc ggatacgctt tcggtgcacg gaacgtcttc tcgtggtcgt tttcgaaata
     5101 gagcactttg taagagtaca caccgctgct gcgcagctta ccaacaatct ctaccggtac
     5161 ggtaccgaga tcttcaccaa tcaccataca gcgatgacgt ttactttcga gtgccagaat
     5221 cgagagcaga tcatccaccg gatagtgaac atacgcgccc tgatctgccg tctcgccata
     5281 cggtatccac cacaaacgca gcatcgacat cacatggtca attcgtaatg cgccgcagtt
     5341 ttgcatattg gcacgcaaca gctcgataaa cggttcatag gcacgcgcgg tgatgatatg
     5401 cgggtccatt ggcggtaatc cccagttctg ccccaacggg ccgaggatat ccggcggcgc
     5461 gccaaccgat gctttcaggc aatatagttc acggtcacac caggtttccg ccccaccttc
     5521 cgctacgcca accgccagat cacgatacaa gccaatcggc atttcatagc cctggcttat
     5581 ctcccagcag gcggcaaact ggctgtaagc cagccactgc aaccagagat aaaaatcgac
     5641 gtcatcacga tgttcttcgc agaactgacg cacttctggt gaatccacgt tcttatacat
     5701 ctctggccat gcaggccagc cccagcgcat ttcgtcctct ttcacttgct gggcatgtag
     5761 cgcatcaaag gcagcctgcc agaacaggct gtcgccctgc tctgcaacaa actggcgaaa
     5821 cgcggccatc tgctcatcat cacgttgcgc gaaacctttc cacgccattc gtaatgctgt
     5881 catttttagg gcggtaaccg tggagtaatc gacccagtcg gcatcgcgcg cctgttgcag
     5941 cgtctgttgc gtggtcggca actgccacca ggcctgagcc tcttcgctaa gatggaaatc
     6001 ttcaacggcg ttaacgtcga tataaatcac attcagccaa cggcgagaag acgggctgta
     6061 tgggctggcg ctctccggat ttgccggata gagcgcatga atcgggttca ggccaatgaa
     6121 cgacccgcca cgttttgcca catccaccag catcgctttg agatcgccaa aatccccaat
     6181 accccagttt ttttccgatc gcagcgtata aagctgaacg caggcacccc acagcttttg
     6241 tttattcagc aaagcctgcg gttcgtaaca acgtttcggg gcgacaatca cccggcaatg
     6301 cgcgcgctgg tcgtcctggg tgagtgttag cgtgtgataa ccttccggca gcttcgtcgg
     6361 tagattgaac gctttgcccc ccgttacatg gcctttgtac tgcgttcctt cttcggtggt
     6421 cagctgccag ctatattcgc cgctgccctc caccaccatc ggcatttttt tgccgctggt
     6481 ataaaccatg acattcggga ctggcgttac cgccactttc gtggcggtac gttgatgcat
     6541 cgcgtcaagc aaacgccgtt tggtttcggc gctaatcgac tgcggtttac cgtgggcatt
     6601 gatgtaattg gggctaatcc ccgccgccag cgcggcatta tccagacgtt tgctttccat
     6661 cgagcttcct tagcgttttg cctgccagat acgagcctga taatcgcgaa tagagcgatc
     6721 cgagctaaac ataccgcagc gggcggtatt gaggatcgcc gcgcgagtcc aggcctcctg
     6781 gtcgcggtac agcacatcca cctgcttttg tgcctcaaca taggctgcga aatccgccat
     6841 caccagatac ggatcgccgc cctgtttgcc gatactgtgc agcatctggt cgaaggcatg
     6901 cttatcgccg tcgctgtatt taccgctttc cagctctttc aatactgcgt ccagcacctt
     6961 atctttcttc cgccatttca ccgggtcgta gcctttggcc agaatagcct tcacttgttc
     7021 cacggtatga ccaaaaataa agatattttc ttcaccgact ttctcggcga tttcaacgtt
     7081 cgcaccatcc agcgtaccga cagtaagcgc accattgagc gccagtttca tattgccggt
     7141 accggaagct tctttacctg cagtcgaaat ttgttcggag atatccgccg ccgggatcag
     7201 tttttccgcc gccgaaacgc aataatccgg caggaacacc accttcaact tatcgccaac
     7261 cagtggatcg ttgttgatca cgtcagccac tttgttgatc gcaaagataa tattcttcgc
     7321 caggtagtag cccggtgccg ctttcgcgcc gaagaggaag acgcgcggta cgcgatcagc
     7381 ctgcaggttt tcacgaattt ctttgtacaa cgccagaata tgcagcagat tcaggtgctg
     7441 gcgtttgtac tcatgcagac gtttgatctg aatatcgaaa atcgcctgtg gattgatctc
     7501 aataccggta cgaactttca caaactccgc cagacagact ttattcgcct gcttgatctc
     7561 gcgatattgc tgacggaatt tcgcatcatc agcgaatttt tccagattga tcagctgatc
     7621 gagatcgtta gcccactctt tttgcagtga tttatccaac agagccgcca gtgcagggtt
     7681 gcactgtttg atccagcgac gtggggtaat accgttggta acgttatgga atttgttcgg
     7741 ccatagctgg tgatattccg ggaacagatc tttcaccacc agatccgagt gcagcgccgc
     7801 aacaccgttc accgcgaaac cgccaaccac acacaggttc gccatatgca cttgtttatc
     7861 gtgcaccacc gccagtttgg cccacacttt ttcatcgccc ggccaggttt tctccaccag
     7921 cgttttaaag cgagtattaa tttcgttaat aatctgcata tggcgcggca gtaagccttt
     7981 caccagtttc acatcccagc gttccagcgc ttctggcatc agggtatggt tggtgtaagc
     8041 gaaagttttg ctggtgatgg cccaggcgtc atcccagctc atctggtgct catcggtcag
     8101 cacgcgcagc agttctggaa tcgcgatagt tggatgggta tcgttcagct gaataacttc
     8161 gtaatccgcc agttcgtgca gtttacgccc cgccagatga tggcgacgca aaatatccgc
     8221 taccgaacag gcacactgga agtattgctg catcaggcgc agctttttac cggcagtatg
     8281 gttgtcgttt ggatagagaa ctttggtcag tttttccgca ttgatgcctt gctgttcggc
     8341 gcgcaggaaa tcaccgtcgt taaatttagt tagatcaaac ggatgcgcat gcgtcgcctg
     8401 ccacagacgc agcggctgcg ccacgccatt acgatagccg acaacgggga gatcccacgc
     8461 ttgaccggta atggtaaact ccggctccca gcgtccgtct ttcatcactt taccgccaat
     8521 ccctacctgc acatccagtg cttcgttgtg gcggaaccac gggtagttac tgcgatgcca
     8581 gtcatccggc gcttcaacct gtttgccatc gacaaaagac tggcggaaca aaccatattg
     8641 atagttcaga ccgtaacccg tcgcagactg accgacagtt gccattgagt cgaggaagca
     8701 cgccgccaga cgtcccagac caccgttacc cagcgccggg tcgatctctt cttccagcag
     8761 gtcagtcagg ttgatgtcat aagccttcaa cgaatcctgt acatcctgat accagccgag
     8821 attcaacagg ttgttgcccg tcagacgacc aatcaaaaac tccattgaga tgtagttaac
     8881 atgtcgctga ttcgccaccg gtttggcgaa tggctgagca cgcagcattt cggccagtgc
     8941 ttcactcact gccagccacc actggcgagg agtcatttca gccgcagaat ttaagccata
     9001 acgctgccac tgacgtgaaa gcacttcctg aaattgctta tcgttaaaaa taggttgtga
     9061 cataggagtt ccacttttct tagattttca acacaacgtt atcgctagtt tgccaggctc
     9121 gatgttgacc ttcctcatcc tgcgggggat taggcaggga ggagttgcgg ggatgagcaa
     9181 ggaaatgtga tctcgaccac ttaaagctag tgcaaaccac aggattagca gcaaatcaat
     9241 gccatacagc gcagaaaatc tgtatctaag tgcaaaaaat ggccgttgcg tattttcaaa
     9301 aagcggaagg taactctata aattaagtaa aggagtgaaa cagtttcaca agtaaaatat
     9361 ccagtgtgct ccatctcatt cttaatagat ttattaagat catcttttta gatggcactt
     9421 tcatcaggaa tgaagaagaa acccttgctt aaatgaatct gatgaacata agggaaacca
     9481 gtattcacgc tggatcagcg tcgttttagg tgagttgtta ataaagattt ggaattgtga
     9541 cacagtgcaa attcagacac ataaaaaaac gtcatcgctt gcattagaaa ggtttctggc
     9601 cgaccttata accattaatt acgaagcgca aaaaaaataa tatttcctca ttttccacag
     9661 tgaagtgatt aactatgctg attccgtcaa aactaagtcg tccggttcga ctcgaccata
     9721 ccgtggttcg tgagcgcctg ctggctaaac tttccggcgc gaacaacttc cggctggcgc
     9781 tgatcacgag tcctgcgggc tacggaaaga ccaccctcat ttcccagtgg gcggcaggca
     9841 aaaacgatat cggctggtac tcgctggatg aaggtgataa ccagcaagag cgtttcgcca
     9901 gctatctcat tgccgccgtg cagcaggcaa ccaacggtca ctgtgcgata tgtgagacga
     9961 tggagcaaaa acggcaatat gccagcctga cgtcactctt cgcccagctt ttcattgagc
    10021 tggcggaatg gcatagccca ctttatctgg tcatcgatga ctatcatctg atcactaatc
    10081 cagtgatcca cgagtcaatg cgcttcttta ttcgccatca accagaaaat ctcaccctgg
    10141 tggtgttgtc acgcaacctt ccgcaactgg gcattgccaa tctgcgtgtt cgtgaccaac
    10201 tgctggaaat tggcagtcag caactggcat ttacccatca ggaagcgaag cagttttttg
    10261 attgccgtct gtcatcgccg attgaagccg cagaaagcag tcggatttgc gatgacgttt
    10321 ccggttgggc gacggcacta tagctaatcg ccctctccgc ccggcagaat acccactcag
    10381 cccataagtc ggcacgccgc ctggcgggaa tcaatgccag ccatctttcg gattatctgg
    10441 tcgatgaggt tttggataac gtcgatctcg caacgcgcca ttttctgttg aaaagcgcca
    10501 ttttgcgctc aatgaacgat gccctcatca cccgtgtgac cggcgaagaa aacgggcaaa
    10561 tgcgtctcga agagattgag cgtcaggggc tgtttttaca gcggatggat gataccggcg
    10621 agtggttctg ctatcacccg ctgtttggca acttcctgcg ccagcgctgc cagtgggaac
    10681 tggcggcgga gctgccggaa atccaccgtg ccgccgcaga aagctggatg gcccagggat
    10741 ttcccagcga agcgattcat catgcgctgg cggcaggcga tgcgctgatg ctgcgcgata
    10801 ttctgcttaa tcacgcctgg agtttgttca accatagcga actgtcgctg ctggaagagt
    10861 cgcttaaggc cctgccgtgg gacagtttgc tggaaaatcc gcagttagtg ttattgcagg
    10921 cgtggctaat gcaaagccaa catcgctatg gcgaagttaa caccctgcta gcccgtgctg
    10981 aacatgaaat caaggacatc agagaaggca ccatgcatgc agaatttaac gctctgcgcg
    11041 cccaggtggc gattaacgat ggtaatcagg atgaagcgga acggctggca aaactggcac
    11101 tggaagagct gccgccgggc tggttctata gccgcattgt ggcaacctcg gtgctgggtg
    11161 aagtgctgca ctgcaaaggc gaattgaccc gctcactggc gctagtgcag caaaccgaac
    11221 agatggcacg ccagcacgac gtctggcact acgccttatg gagtttaatt cagcaaagtg
    11281 aaattctgtt tgcccaaggg ttcctgcaaa ccgcgtggga aacgcaggaa aaagcgttcc
    11341 agctgatcaa cgagcagcat ctggaacagc tgccaatgca tgagtttctg gtgcgcatcc
    11401 gagcacaact gttatgggcc tgggcacggc tggatgaagc cgaagcgtcg gcgcgtagcg
    11461 ggattgaagt cttgtcgtct tatcagccac agcaacagct tcagtgcctg gcaatgttga
    11521 ttcaatgctc gctggcccgt ggtgatttag ataacgcccg tagccagttg aaccgtctgg
    11581 aaaacctgct ggggaatggc aaatatcaca gcgactggat ctctaacgcc aacaaagtcc
    11641 gggtgattta ctggcaaatg accggcgata aagccgccgc tgccaactgg ttgcgtcata
    11701 cggctaaacc ggagtttgcg aacaaccact tcctgcaagg tcaatggcgc aacattgccc
    11761 gcgcacaaat cttgctgggc gagtttgaac ctgcggaaat tgttctcgaa gaactcaatg
    11821 aaaatgcccg cagtctgcgg ttgatgagcg atctcaaccg taacctgttg ctgcttaatc
    11881 aactgtactg gcaggccgga cgtaaaagtg acgcccagcg cgtgttgctg gacgcattaa
    11941 aactggcgaa tcgcaccgga tttatcagtc attttgtcat cgaaggcgaa gcgatggcgc
    12001 aacaactgcg ccagctgatt caacttaata cgctgccgga actggaacag catcgcgcgc
    12061 agcgtattct gcgagaaatc aatcaacatc atcggcataa attcgcccat ttcgatgaga
    12121 atttcgttga acgtctgcta aatcatccgg aagtgccaga actaatccgc accagcccgt
    12181 tgacccaacg tgaatggcag gtcctggggc tgatctactc cggttacagc aatgaacaaa
    12241 ttgcgggcga actggaagtg gcggcaacca ccatcaagac gcatatccgc aatctgtacc
    12301 aaaaactcgg cgtcgcccac cgtcaggctg ccgtgcagca cgcgcagaaa ctgctgaaga
    12361 tgatgggtta tggggtgtga gtttagccgg ataatgcatt ggatccggtt tacatcttta
    12421 catcattcaa tactcacccg cgttacgcca tctgtttcta tcaaaccaaa ccgcaccggc
    12481 aagaaacgct ccaccaccgc gatattggtc agcagatggc atgaaggatg ggcgaccgta
    12541 aattctcccg cgcccgccag cgccatcggc agtaccagtt ggtcggcgag atattccccc
    12601 accgccgctg ggcttgccag gtagcgtttc acctctttca ctaactgcgc ggctaccacc
    12661 tcggcactga cgcgcttttc accgacgacg aaaaagcgtt cagtgatatt ttcactttcg
    12721 acttccagcg agacggtatt acccggcccc tggtcgcgcg gcaggttatg aatattctgt
    12781 tcatgcaggg aaaaaatccc cgccagtgta gcgatttcac gctcagcaac atggcgcggc
    12841 acaccagcta atagaacttc tccacgcatc tgcacaatgt tcccgcgctc gccaagttgc
    12901 aaggtgctaa acgatgccac gggcgagact tccgttgcca ccacaccgcc tccggcagga
    12961 taaaaaccgt gacgtaacag cgtggtttgc tgatgaattc ctattttcgc cagcagcggc
    13021 tccagcaccc ggcggataaa atccgcaggc ggcgccgacg ggttatcggt gccgccgctc
    13081 acttcaacac gcgaaggtcc atcggcaaac cacagcgcgg gcagcaccgt ttgcagcacc
    13141 agcgtacaac ttccggcgct accgatagca aagcggtaat cgccgccgcg cacggtgcca
    13201 ggccggaaga ccagacgctg cgatcccagc tccgcgcctt ccaccgttgc cctacaaatt
    13261 tccgcagccg cttttaccgc ggtcagatgc tggcgcagca gccccggttt cgcccgtccg
    13321 gctcgaatgc cagtgatggt aaatggctgg ccggttatca tcgacaggct cagcgccgag
    13381 cgcaggatct gcccgccgcc ttcgccctgt gcgccatcca gcgcaatcat ccttttcatc
    13441 atttatcctt ttacgcacac cacctgacgc agggtgtaga taacttccac cagatcgctt
    13501 tgtgccgcca tcaccgcatc aatatcttta tacgccatcg ggatttcgtc gatcacttcg
    13561 gcatctttac ggcattccac atgcgcggtg gcacgaattt ggtcttccac gctgaacagt
    13621 tttttcgctt tagttcggct cattacccgc ccggcaccgt ggctgcacga acagaacgac
    13681 tcttcatttc ccagcccacg gacgataaag ctttttgctc ccatcgaacc gggaataatt
    13741 ccatattgac cagcacgcgc agacaccgca cctttacgcg tcacgtagat ctcttcacca
    13801 aagagctgtt ctttttgcac atagttgtgg tgacagttga tctcttccat cgccagcgtt
    13861 tgtggctgtc tgaccgtttt ctgcgtaatg ctctgcaacg ccgttaccac attttccatc
    13921 atcgcatcgc ggttaaggct ggcgaaaagc tgcgcccagg ccacggctct taggtaatca
    13981 tcaaagtatt ccgtaccttc cataaagtac gccagatcac gcgacggcaa cgtctcaagt
    14041 tggtcctgca tctctttttg cgccagatcg ataaagtaag tcccgatggc gttaccaatt
    14101 ccgcgtgaac cggagtgcag cataatccac acctgctccg actcatcaag gcagatttca
    14161 ataaagtggt tacccgttcc cagcgttccc aggtgtttat agttgttagt gttaaggaaa
    14221 cgcggatatt tttgcgttaa ccactgataa ccggcctcca gctcagccca tttcgcatcg
    14281 acgttaaaag gcgggttttc ccatgcgcct ttatcacgtt tacaacgccc ggtagtacgc
    14341 ccgtgtggta cggccgtttc aatcgcttga cgcagctccg ccaggttttc aggcaggtct
    14401 gccgccgtta acgcggtacg cagcgcgttc attccacagc caatatccac gcccaccgcc
    14461 gccggaataa tcgccccttt ggtcgggatc acgctaccaa tggtggaacc tttacccagg
    14521 tgtacatcag gcattaccgc aatatgtttg aaaataaacg gcatcttcgc cgtattaata
    14581 agttgctgac gcgcatcggc ttctaccggc acgcctttgg tccacatttt taccggggca
    14641 ttttcagtgg tcagtaattc gtaattcatt ttgtttttct cttttcgttg tttgcggtcc
    14701 tgataattgc aaccgtcgtg ccagaaaatt aaataagcag attaatttat tgtttctaaa
    14761 aagattatat ctttatatcc gtaaacggag atttcccgca aagccgattt accgataatg
    14821 aaatatcgcc ttttataagg atatctaaga tgcgtaaaac agtggctttt ggctttgtcg
    14881 gtaccgtgct ggattatgcc ggacgcggca gtcagcgctg gtcaaaatgg cgtccgtcac
    14941 tctgtatata ccagcaagaa tcgttggtca tcgatcgact ggaattattg cacgatgccc
    15001 gctcgcgctc gctatttgaa acgcttaaac gcgatatcgc cagcgtttcg ccagaaacag
    15061 aagtggtggg cgttgagatt gaactgcata acccgtggga tttcgaagag gtttacgcct
    15121 gcctgcatga tttcgcccgt ggttacgagt ttcagccaga aaaagaagac tatttaattc
    15181 acatcaccac cggcacccac gtcgcgcaga tttgctggtt tctgctggcg gaagcgcgtt
    15241 atctacccgc ccggttgata caatcttcaa ctccgcgcaa aaaagaacag ccgcgcggcc
    15301 caggtgaagt gacgattatc gatctcgatt taagccgtta taacgccatc gccagccgct
    15361 ttgccgagga acggcagcaa acgctcgatt tcctcaaatc cggtattgcc acgcgtaacc
    15421 cccacttcaa ccgcatgatt gagcagatcg aaaaagtggc gattaaatcc cgcgcgccga
    15481 ttctgcttaa cggcccgacc ggcgcaggta agtcatttct ggcgcgacgc atcttcgagt
    15541 taaaacaggc acggcatcag tttagcggcg cgtttgtgga agtgaactgc gccaccctgc
    15601 gcggcgatac cgcgatgtcg acgctgtttg gtcatgtgaa aggcgcgttt accggggcgc
    15661 gggaatctcg tgaaggatta ttacgcagcg ccaacggcgg aatgttgttt cttgatgaga
    15721 ttggcgaact gggcgcagac gaacaggcaa tgctgctgaa agctattgaa gagaaaacct
    15781 tttacccgtt tggcagcgat cgccaggtga gcagtgattt tcagcttatc gccggaacgg
    15841 tgcgcgattt gcgccagctg gttgccgaag gcaaattccg cgaagatctg tacgcgcgga
    15901 tcaatctctg gaccttcacc ctgccgggtc tacgccagcg ccaggaagat attgaaccga
    15961 acctggatta tgaagtggag cgccacgcca cactcactgg tgacagcgtg cgttttaaca
    16021 ccgaagcgcg gcgtgcctgg ctggcctttg caacatctcc ccaggcaaca tggcgcggta
    16081 actttcgcga actttctgcc agcgtcacgc ggatggccac ctttgccact agcggacgca
    16141 tcactctgga cgtggtggaa gatgagataa accgtctgcg ctataactgg caggagagcc
    16201 gcccctccgc gcttacggcg ttgctgggcg cagaggcaga aaacatcgat ctcttcgacc
    16261 gtatgcaact ggaacacgtt atcgctatct gccgccaggc aaagtcgctt tctgctgccg
    16321 gacgacagct ttttgatgtt tcgcgccagg gcaaagccag cgtcaatgac gcggatcggc
    16381 tacgcaaata cctggcgcgt tttggtctga cgtgggaagc cgtgcaggat cagcacagct
    16441 ccagttgaat atggtggtcc gtcagcacct gcatcacgct tactggcggc ggggcgtcgg
    16501 tgtagacggc atcaaccatg ctgatgctcc ccatattgac catcgcatta cggccaaatt
    16561 ttgaatggtc aaccaccagc ataacgtggc gcgagttctc aataatggcg cgcttggtac
    16621 gaacttcgtg gtaatcgaac tccagcagcg agccgtcgct gtcgatgccg ctgatcccca
    16681 gaatgccgaa atcaaggcgg aactgggaga taaaatcgag cgtcgcttcg ccaatgatcc
    16741 cgccatcgcg gctacgtaat tcgccaccgg cgagaataat gcggaaatct tcttttacca
    16801 tcaacgtgtt agcaacgttg agattgttgg tgacaatgcg caaattgctg tgattgagca
    16861 gcgcgtgcgc caccgcttcc ggcgtggtgc cgatatcgat aaacagcgtc gagccattgg
    16921 ggatttgctc cgccactttg cgggcgatgc gctctttttc ttcggtctgg gtggctttac
    16981 gatcgtgcca cggcgtgtta accgaactgg aaggcagcgc cgcgccgcca tgatggcgca
    17041 ggatcaggtt ttgctctgcc agctcgttga ggtcgcggcg aatagtctgc gggctgacgg
    17101 agaagtgctc caccagttcc tcggtactga cataaccctg ctgtttaacc agttcgataa
    17161 taccgttgtg acgttgtgtt tgtttcattt ataaatccct ggaattattt tcgttttcgc
    17221 gcattgagcg aatcaacaaa agccatcgct aaacccacgg ctaatccggc gatgtgtgct
    17281 ccgttcgcca tcgacatccc aaacaaatca aaccatccgg cgacaatcca gatcagcgca
    17341 aagataatta acccacgttg caggtaaatg ccactttgcg gatcgcgttc gccacgtagc
    17401 cagacgtagc ccatcagcgc atacaccacg ccagaaagcc cgccaaacca cggcccgctg
    17461 aatttttgct gcatatagcc gcttaacagg gcgctgataa gcgtaatgac aattagctta
    17521 ccgctaccga ggcgtttttc caccgcgccg ccgagatacc accaccaaag caggttaaag
    17581 aggatatgca tcagcgagaa gtgcattaac gcgtgggtga agtaacgcca gaactcaaat
    17641 ttcagtgttg gatcgaatgg ccaggccagc cataacatca cttcctgatc gccgagaatt
    17701 tgcatggcaa taaacaccac cacgcaggcg atcatcatca cccaggtcac cggacctgcg
    17761 cgttcacgca aggcagcaaa gaaaggataa cggcgataat gcaggccact gccggtatgg
    17821 cctgccagcc agctggccgc cagataacgc ggatctgccg ggttttcgag aaaacgcgcc
    17881 agctccgccc gcacgcgctc ggcctgggac tcatccgcca gccagacatc gctttggtta
    17941 tgttgttgaa tcgtgaggat aacaccctgc gtcgccatgt aatcaacaaa cgcctgcgcc
    18001 acgcgggggt tagcaaaaga ggtaatcatc aacatcgttg ctgtcgctta ttccacacaa
    18061 aaggggacag tataaagcgt tacgcgccgt acgccacctc tgcgggaaac tgtcgctgcc
    18121 aggcttcaaa gccgccgtca atgctataga ccacatcgta gccctgttgc agcagatact
    18181 gcgccgcccc tttgctgcta ttgccgtgat aacacatcac catcaccgga gtgtcaaagt
    18241 cgttatcacg cataaacgcg cccagcgtgt cgttggttaa atggaaagcc tgcaccgcat
    18301 gccccatagc gaaactctgt ggatcgcgaa tatcgaccag caccgcctct ttttcctgca
    18361 acttctggtg cgcgtcggca acgttaatac attcgaactg atccatgcgt ctctctttct
    18421 ttacaaacaa gtgggcaaat ttaccgcaca gtttacgtcg aagcggcaga taaacgccat
    18481 aatgttatac atatcactct aaaatgtttt ttcaatgtta cccaaagcgc gattctttgc
    18541 taatatgttc gataacgaac atttatgagc tttaacgaaa gtgaatgagg gcagcatgga
    18601 aaccaaagat ctgattgtga taggtggcgg catcaatggt gctggtatcg cggcagacgc
    18661 cgctggacgc ggtttatccg tgctgatgct ggaggcgcag gatctcgctt gcgcgacctc
    18721 ttccgccagt tcaaaactca ttcacggtgg cctgcgctac cttgagcact atgaattccg
    18781 cctggtcagc gaggcgctgg ctgaacgtga agtgctgctg aaaatggctc cgcatatcgc
    18841 cttcccgatg cgttttcgcc tgccacatcg tccgcatctg cgcccggcgt ggatgattcg
    18901 cattggtctg tttatgtacg atcatctggg taaacgcacc agcctgccgg gatcaactgg
    18961 tttgcgtttt ggcgcaaatt cagtgttaaa accggaaatt aagcgcggat tcgaatattc
    19021 tgactgttgg gtagacgacg cccgtctggt actcgccaac gcccagatgg tggtgcgtaa
    19081 aggcggcgaa gtgcttactc ggactcgcgc cacctctgct cgccgcgaaa acggcctgtg
    19141 gattgtggaa gcggaagata tcgataccgg caaaaaatat agctggcaag cgcgcggcct
    19201 ggttaacgcc accggcccgt gggtgaaaca gttcttcgac gaggggatgc atctgccttc
    19261 gccttatggc attcgcctga tcaaaggcag ccatattgtg gtgccgcgcg tgcataccca
    19321 aaagcaggcc tacattctgc aaaacgaaga taaacgcatt gtgttcgtga tcccgtggat
    19381 ggacgagttt tccatcatcg gcaccaccga tgtggaatac aaaggtgatc cgaaagcagt
    19441 gaagattgaa gagagtgaaa tcaattacct gctgaaagtg tataacgcgc actttaaaaa
    19501 gcagctaagc cgtgacgata tcgtctggac ctactccggt gtgcgtccgc tgtgtgatga
    19561 tgagtccgac tcgccgcaag ccattacccg tgattacacc cttgatatcc atgatgaaaa
    19621 cggcaaagca ccgctgctgt cggtattcgg cggtaagctg accacctacc gaaaactggc
    19681 agaacatgcg ctggaaaaac tgacgccgta ttatcagggt attggcccgg cgtggacgaa
    19741 agagagcgtg ctgccgggtg gtgccattga aggcgaccgc gacgattacg ccgctcgcct
    19801 gcgccgccgt tacccgttcc tgactgaatc gctggcacgt cactacacgc gcacttacgg
    19861 cagcaacagc gagctgctgc tcggcaatgc gggaacggta agcgatctcg gggaagattt
    19921 cggtcatgag ttctacgaag cggaactgaa atacctggtg gatcacgaat gggtccgccg
    19981 caccgacgat gccctgtggc gtcgcacaaa acaaggcatg tggctgaatg cggatcaaca
    20041 atctcgtgtg agtcagtggc tggtggaata tacgcagcag aagttgtcgc tggcgtcgta
    20101 aattaacgta aggtgatcag gtcagatttc aatctggcct gagactgatg acaaacacaa
    20161 aactgcctga tgcgctacgc ttatcaggcc tacgtggttt atgcaatata ttgaatttgc
    20221 atggtcttgt aggccagata agacgttcac gtcgcatccg gcatgaacaa agcgcacttt
    20281 gtcaaaaatc taacctactt tttaattcag gaaattaccg caaagcccac gccatcatac
    20341 ccaacgtaac aaaactcagg cacgttcccc actcgccccg agaaaaatag cattaatgcg
    20401 cccagcgtca gcataaaaat attgagcaga aggtaaattg ttggcgtaaa gagtaaattg
    20461 caaacctcag gaatagcggc aagcccagcc cccagcatgg caataaccgc tgcaaaaaat
    20521 tgcgtcagta ttgggatgcc gaaaaaagtc attaccagcg aggtcaaaat ccattttcta
    20581 ttttgcatta ttctttccat tctttctgaa tggcgaaatt atactccccg agtccccttg
    20641 ccccttctgg acacttttcc gaaatgatgg cgaaaaaaac gggacccttt gg
//
LOCUS       DAJTRC010000068        20676 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.068, whole genome shotgun sequence.
ACCESSION   DAJTRC010000068 DAJTRC010000000
VERSION     DAJTRC010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20676)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20676)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20676
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.068"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            97..396
                     /locus_tag="OO357_002685"
     CDS             97..396
                     /locus_tag="OO357_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5887582.1"
                     /translation="MTELAQLQASTEQAAALLKAMSHPKRLLILCMLSGSPGTSAGEL
                     TRITGLSASATSQHLARMRDEGLINSQRDAQRILYSIKNEAVNAIIATLKNVYCP"
     gene            406..930
                     /gene="ygaP"
                     /locus_tag="OO357_002686"
     CDS             406..930
                     /gene="ygaP"
                     /locus_tag="OO357_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YgaP"
                     /protein_id="HCR5887583.1"
                     /translation="MALTTISPHDAQELIARGAKLIDIRDADEYLREHIPEADLAPLS
                     VLEQSGLPPKLRREQIIFHCQAGKRTSNNADKLAAIAAPAEIFLLEDGIDGWKRAGLP
                     VAVNKSQPLPLMRQVQIAAGGLILNGVVLGYTVNSGFFLLSGFVGAGLLFAGISGFCG
                     MARLLDKMPWNQRA"
     gene            complement(977..1381)
                     /gene="stpA"
                     /locus_tag="OO357_002687"
     CDS             complement(977..1381)
                     /gene="stpA"
                     /locus_tag="OO357_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein StpA"
                     /protein_id="HCR5887584.1"
                     /translation="MSVMLQSLNNIRTLRAMAREFSIDVLEEMLEKFRVVTKERREEE
                     EQQQRELAERQEKISTWLELMKADGINPEELLGNSSAAAPRAGKKRQPRPAKYKFTDV
                     NGETKTWTGQGRTPKPIAQALAEGKSLDDFLI"
     gene            2049..2498
                     /gene="alaE"
                     /locus_tag="OO357_002688"
     CDS             2049..2498
                     /gene="alaE"
                     /locus_tag="OO357_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-alanine exporter AlaE"
                     /protein_id="HCR5887585.1"
                     /translation="MFSPQSRLRHAVADTFAMVVYCSVVNMCIEVFLSGMSFEQSFYS
                     RLVAIPVNILIAWPYGMYRDLFMRAARKVSPSGWIKNLADILAYVTFQSPVYVAILLV
                     VGADWHQIMAAVSSNIVVSMLMGAVYGYFLDYCRRLFKVSRYQQVKA"
     gene            complement(2535..2879)
                     /locus_tag="OO357_002689"
     CDS             complement(2535..2879)
                     /locus_tag="OO357_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgaC family protein"
                     /protein_id="HCR5887586.1"
                     /translation="MYLRPDEVARVLEKVGFTVDVVTQKAYGYRRGENYVYVNREARM
                     GRTALVIHPTLKERSSTLAEPASDIKTCDHYQQFPLYLAGERHEHYGIPHGFSSRVAL
                     ERYLNGLFGEAS"
     gene            3031..3261
                     /locus_tag="OO357_002690"
     CDS             3031..3261
                     /locus_tag="OO357_002690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000282763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887587.1"
                     /translation="MFNRPNRNDVDDGVQDIQNDVNQLADSLESVLKSWGSDAKGEAE
                     AARSKAQALLKETRARMHGRTRVQQAARDAVG"
     gene            3608..3853
                     /gene="nrdH"
                     /locus_tag="OO357_002691"
     CDS             3608..3853
                     /gene="nrdH"
                     /locus_tag="OO357_002691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HCR5887588.1"
                     /translation="MRITIYTRNDCVQCHATKRAMENRGFDFEMINVDRVPEAAEALR
                     AQGFRQLPVVIAGDLSWSGFRPDMINRLHPAPHAASA"
     gene            3850..4260
                     /gene="nrdI"
                     /locus_tag="OO357_002692"
     CDS             3850..4260
                     /gene="nrdI"
                     /locus_tag="OO357_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HCR5887589.1"
                     /translation="MSQLVYFSSSSENTQRFIERLGLPAVRIPLNERERIQVDEPYIL
                     IVPSYGGGGTAGAVPRQVIRFLNDEHNRALLRGVIASGNRNFGEAYGRAGDVIARKCG
                     VPWLYRFELMGTQSDIENVRKGVTEFWQRQPQNA"
     gene            4233..6377
                     /gene="nrdE"
                     /locus_tag="OO357_002693"
     CDS             4233..6377
                     /gene="nrdE"
                     /locus_tag="OO357_002693"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HCR5887590.1"
                     /translation="MATTTAECLTQETMDYHALNAMLNLYDSAGRIQFDKDRQAVDAF
                     IATHVRPNSVTFSSQQQRLNWLVNEGYYDESVLNRYSRDFVITLFAHAHTSGFRFQTF
                     LGAWKFYTSYTLKTFDGKRYLEDFADRVTMVALTLAQGDETLALQLTDEMLSGRFQPA
                     TPTFLNCGKQQRGELVSCFLLRIEDNMESIGRAVNSALQLSKRGGGVAFLLSNLREAG
                     APIKRIENQSSGVIPVMKMLEDAFSYANQLGARQGAGAVYLHAHHPDILRFLDTKREN
                     ADEKIRIKTLSLGVVIPDITFHLAKENAQMALFSPYDVERVYGKPFADIAISEHYDEL
                     VADERIRKKYLNARDFFQRLAEIQFESGYPYIMYEDTVNRANPIAGRINMSNLCSEIL
                     QVNSASEYDENLDYARTGHDISCNLGSLNIAHTMDSPDFSRTVETAVRGLTAVSDMSH
                     IRSVPSIEAGNAASHAIGLGQMNLHGYLAREGIAYGSPEALDFTNLYFYTITWHALRT
                     SMLLARERGETFAGFKQSRYASGEYFSQYLQGNWQPKTAKVGELFARSCITLPTREMW
                     AQLRDDVMRYGIYNQNLQAVPPTGSISYINHATSSIHPIVAKVEIRKEGKTGRVYYPA
                     PFMTNENLALYQDAYEIGAEKIIDTYAEATRHVDQGLSLTLFFPDTATTRDINKAQIY
                     AWRKGIKTLYYIRLRQMALEGTEIEGCVSCAL"
     gene            6387..7346
                     /gene="nrdF"
                     /locus_tag="OO357_002694"
     CDS             6387..7346
                     /gene="nrdF"
                     /locus_tag="OO357_002694"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HCR5887591.1"
                     /translation="MKLSRISAINWNKISDDKDLEVWNRLTSNFWLPEKVPLSNDIPA
                     WQTLTVVEQQLTMRVFTGLTLLDTLQNVIGAPSLMPDALTPHEEAVLSNISFMEAVHA
                     RSYSSIFSTLCQTKDVDAAYAWSEENAPLQRKAQIIQQHYRGDDPLKKKIASVFLESF
                     LFYSGFWLPMYFSSRGKLTNTADLIRLIIRDEAVHGYYIGYKYQKNMEKISLGQREEL
                     KSFAFDLLLELYDNELQYTDELYAETPWADDVKAFLCYNTNKALMNLGYEPLFPAEMA
                     EVNPAILAALSPNADENHDFFSGSGSSYVMGKAVETEDEDWNF"
     gene            7701..8903
                     /gene="proV"
                     /locus_tag="OO357_002695"
     CDS             7701..8903
                     /gene="proV"
                     /locus_tag="OO357_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein ProV"
                     /protein_id="HCR5887592.1"
                     /translation="MAIKLKIKNLYKIFGEHPQRAFKYIEQGLSKEQILEKTGLSLGV
                     KDASLAIEEGEIFVIMGLSGSGKSTMVRLLNRLIEPTRGQVLIDGVDIAKISDAELRE
                     VRRKKIAMVFQSFALMPHMTVLDNTAFGMELAGINAEERREKALDALRQVGLENYAHS
                     YPDELSGGMRQRVGLARALAINPDILLMDEAFSALDPLIRTEMQDELVKLQAKHQRTI
                     VFISHDLDEAMRIGDRIAIMQNGEVVQVGTPDEILNNPANDYVRTFFRGVDISQVFSA
                     KDIARRTPNGLIRKTPGFGPRSALKLLQDEDREYGYVIERGNKFVGAVSIDSLKTALT
                     QQQGLDAALIDAPLAVDAQTPLSELLSHVGQAPCAVPVVDEDQQYVGIISKGMLLRAL
                     DREGVNNG"
     gene            8896..9960
                     /gene="proW"
                     /locus_tag="OO357_002696"
     CDS             8896..9960
                     /gene="proW"
                     /locus_tag="OO357_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     permease ProW"
                     /protein_id="HCR5887593.1"
                     /translation="MADQNNPWDTTPAADSAAQSADAWGTPTTAPADGGGADWLTSTP
                     APNVEHFNILDPFHKTLIPLDSWVTEGIDWVVTHFRPVFQGVRVPVDYILNGFQQLLL
                     GMPAPVAIIVFALITWQISGVGMGVATLVSLIAIGAIGAWSQAMVTLALVLTALLFCI
                     VIGLPLGIWLARSPRAAKIIRPLLDAMQTTPAFVYLVPIVMLFGIGNVPGVVVTIIFA
                     LPPIIRLTILGINQVPADLIEASRSFGASPRQMLFKVQLPLAMPTIMAGVNQTLMLAL
                     SMVVIASMIAVGGLGQMVLRGIGRLDMGLATVGGVGIVILAIILDRLTQAVGRDSRSR
                     GNRRWYTTGPVGLLTRPFIK"
     gene            10018..11010
                     /gene="proX"
                     /locus_tag="OO357_002697"
     CDS             10018..11010
                     /gene="proX"
                     /locus_tag="OO357_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708493.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     substrate-binding protein ProX"
                     /protein_id="HCR5887594.1"
                     /translation="MRHSVLFATAFATLISTQTFAADLPGKGITVNPVQSTITEETFQ
                     TLLVSRALEKLGYTVNKPSEVDYNVGYTSLASGDATFTAVNWTPLHDNMYEAAGGDKK
                     FYREGVFVNGAAQGYLIDKKTADQYKITNIAQLKDPKIAKLFDTNGDGKADLTGCNPG
                     WGCEGAINHQLAAYELTNTVTHNQGNYAAMMADTISRYKEGKPVFYYTWTPYWVSNEL
                     KPGKDVVWLQVPFSALPGDKNADTKLPNGANYGFPVSTMHIVANKAWTEKNPAAAKLF
                     AIMQLPVADINAQNAIMHDGKASEGDIQGHVDGWIKAHQQQFDGWVNEALAAQK"
     gene            11202..12381
                     /locus_tag="OO357_002698"
                     /pseudo
     CDS             11202..12381
                     /locus_tag="OO357_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311570.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12505..13242
                     /gene="ygaZ"
                     /locus_tag="OO357_002699"
     CDS             12505..13242
                     /gene="ygaZ"
                     /locus_tag="OO357_002699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine exporter subunit YgaZ"
                     /protein_id="HCR5887595.1"
                     /translation="MESPTPQPAPGSATFMEGCKDSLPIVISYIPVAFAFGLNATRLG
                     FSPLESVFFSCIIYAGASQFVITAMLAAGSSLWVAALSVMAMDVRHVLYGPSLRSRII
                     QRLQKSKTALWAFGLTDEVFAAATAKLVRNNRRWSENWMIGIAFSSWSSWVFGTVIGA
                     FSGSGLLQGYPAVEAALGFMLPALFMSFLLASFQRKQSLCVTAALVGALAGVTLFSIP
                     VAILAGIVCGCLTALIQAFWQGAPDEL"
     gene            13232..13567
                     /gene="ygaH"
                     /locus_tag="OO357_002700"
     CDS             13232..13567
                     /gene="ygaH"
                     /locus_tag="OO357_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-valine transporter subunit YgaH"
                     /protein_id="HCR5887596.1"
                     /translation="MSYEVLLLGLLVGAANYCFRYLPLRLRVGNARPTKRGAVGILLD
                     TIGIASICALLVVSTAPEVMHDTRRFVPTLVGFAVLGASFYKTRSIIIPTLLSALAYG
                     LAWKVMAII"
     gene            13658..14188
                     /gene="emrR"
                     /locus_tag="OO357_002701"
     CDS             13658..14188
                     /gene="emrR"
                     /locus_tag="OO357_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux transporter EmrAB
                     transcriptional repressor EmrR"
                     /protein_id="HCR5887597.1"
                     /translation="MDSSFTPIEQMLKFRASRHEDFPYQEILLTRLCMHMQSKLLENR
                     NKMLKAQGINETLFMALITLESQENHSIQPSELSCALGSSRTNATRIADELEKRGWIE
                     RRKSDNDRRCLHLQLTEKGHEFLREVLPPQHNCLHQLWSALSTTEKDQLEQITRKLLS
                     RLDQMEQDGVVLEAMS"
     gene            14315..15487
                     /gene="emrA"
                     /locus_tag="OO357_002702"
                     /pseudo
     CDS             14315..15487
                     /gene="emrA"
                     /locus_tag="OO357_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311574.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter periplasmic
                     adaptor subunit EmrA"
     gene            15504..17042
                     /gene="emrB"
                     /locus_tag="OO357_002703"
     CDS             15504..17042
                     /gene="emrB"
                     /locus_tag="OO357_002703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708498.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter permease
                     subunit EmrB"
                     /protein_id="HCR5887598.1"
                     /translation="MQQQKPLEGAQLVIMTIALSLATFMQVLNSTIANVAIPTIAGNL
                     GSSLSQGTWVITSFGVANAISIPLTGWLAKRVGEVKLFLWSTIAFAIASWACGVSSSL
                     NMLIFFRVIQGIVAGPLIPLSQSLLLNNYPPAKRSIALALWSMTVIVAPICGPILGGY
                     ISDNYHWGWIFFINVPIGVAVVLMTLQTLRGRETRTERRRIDAVGLALLVIGIGSLQI
                     MLDRGKELDCFSSQEIIILTVVAVVAICFLIVWELTDDNPIVDLSLFKSRNFTIGCLC
                     ISLAYMLYFGAIVLLPQLLQEVYGYTATWAGLASAPVGIIPVILSPIIGRFAHKLDMR
                     RLVTFSFIMYAVCFYWRAYTFEPGMDFCASAWPQFIQGFAVVCFFMPLTTITLSGLPP
                     ERLAAASSLSNFTRTLAGSIGTSITTTMWTNRESMHHAQLTESVNPFNPNAQAMYSQL
                     EGLGMTQQQASGWIAQQITNQGLIISANEIFWMSAGIFLVLLGLVWFAKPPFGAGGGG
                     GGAH"
     gene            complement(17106..17621)
                     /gene="luxS"
                     /locus_tag="OO357_002704"
     CDS             complement(17106..17621)
                     /gene="luxS"
                     /locus_tag="OO357_002704"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008458247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HCR5887599.1"
                     /translation="MPLLDSFTVDHTRMEAPAVRVAKTMNTPHGDAITVFDLRFCVPN
                     KEVMPERGIHTLEHLFAGFMRNHLNGNGVEIIDISPMGCRTGFYMSLIGTPDEQRVAD
                     AWKAAMEDVLKVQDQNQIPELNVYQCGTYQMHSLQEAQDIARSILEREVRINSNEELA
                     LPKEKLQELHI"
     gene            complement(17771..19327)
                     /gene="gshA"
                     /locus_tag="OO357_002705"
     CDS             complement(17771..19327)
                     /gene="gshA"
                     /locus_tag="OO357_002705"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5887600.1"
                     /translation="MIPDVSQALAWLEKHPQALKGIQRGLERETLRVNADGTLATTGH
                     PEALGSALTHKWITTDFAEALLEFITPVDGDIEHMLTFMRDLHRYTARNMGDERMWPL
                     SMPCYIAEGQDIELAQYGTSNTGRFKTLYREGLKNRYGALMQTISGVHYNFSLPMAFW
                     QAKCGDISGADAKEKISAGYFRVIRNYYRFGWVIPYLFGASPAICSSFLQGKPTSLPF
                     EKTECGMYYLPYATSLRLSDLGYTNKSQSNLGITFNDLYEYVAGLKQAIKTPSEEYAK
                     IGIEKDGKRLQINSNVLQIENELYAPIRPKRVTRSGESPSDALLRGGIEYIEVRSLDI
                     NPFSPIGVDEQQVRFLDLFMVWCALADAPEMSSSELACTRVNWNRVILEGRKPGLTLG
                     IGCETAQFPLLQVGKDLFRDLKRVAQTLDSINGGEAYQKVCDELVACFDNPDLTFSAR
                     ILRSMIDTGIGGTGKAFAEAYRNLLREEPLEILREEDFVAEREASERRQQEMETADTE
                     PFAVWLEKHA"
     gene            complement(19400..19828)
                     /locus_tag="OO357_002706"
     CDS             complement(19400..19828)
                     /locus_tag="OO357_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HCR5887601.1"
                     /translation="MSEALSLFSLFASSFLSATLLPGNSEVVLVAMLLSGISHPWVLV
                     LTATMGNSLGGLTNVILGRFFPLRKTSRWQEKATGWLKRYGAVTLLLSWMPVVGDLLC
                     LLAGWMRISWGPVIFFLCLGKALRYVAVAAATVQGMMWWH"
     gene            complement(19825..20391)
                     /gene="yqaB"
                     /locus_tag="OO357_002707"
     CDS             complement(19825..20391)
                     /gene="yqaB"
                     /locus_tag="OO357_002707"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-1-phosphate/6-phosphogluconate
                     phosphatase"
                     /protein_id="HCR5887602.1"
                     /translation="MYERYAGLIFDMDGTILDTAPTHRKAWREVLGHYGLQYDIQAMI
                     ALNGSPTWRIAQAIIELNQADLDPHALAREKTEAVRSMLLDSVEPLPLVEVVKSWHGR
                     RPMAVGTGSESAIAEALLAHLGLRRYFDAVVAADHVKHHKPAPDTFLLCAQRMGVQPT
                     QCVVFEDADFGIQAARAAGMDAVDVRLL"
     gene            complement(20539..20615)
                     /locus_tag="OO357_002708"
     tRNA            complement(20539..20615)
                     /locus_tag="OO357_002708"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20579..20581),aa:Arg,seq:acg)
BASE COUNT         4994 a         5287 c         5159 g         5236 t
ORIGIN      
        1 cagcttacgc ttttactctc atttacgctg ctgaggctgg tatatgttgc atttttagtt
       61 tagagtttac ttaatttaga aaatacttaa ataaatatga ctgaactcgc gcaattacag
      121 gccagtaccg aacaggcagc ggccttattg aaagcaatga gccaccctaa acggttgctg
      181 attctgtgca tgcttagcgg ttcccccggc accagcgcgg gagagctgac gcgcattacc
      241 ggactgagcg cctctgcgac atcacagcat ctcgctcgta tgcgggacga agggcttatc
      301 aacagccaac gggatgccca acgcattcta tattccatta aaaatgaggc ggtaaatgcc
      361 attatcgcca ccctgaaaaa tgtctattgt ccgtaaggag tcactatggc tttgacaacc
      421 atttcgccgc atgatgcaca agaattaatc gcacgcggcg caaagttaat cgatattcgt
      481 gacgctgatg aatatcttcg tgaacatatt cctgaagcag atctggctcc attatccgtg
      541 ctggaacagt caggtcttcc acctaaatta cgtcgcgagc aaattatatt ccactgccag
      601 gcaggtaaac gcaccagtaa taacgccgat aaattagcag cgattgccgc ccccgcagaa
      661 atatttttac tcgaagatgg gattgatggc tggaaaagag ctggattgcc agtagcggta
      721 aataaatctc aacccttgcc gttaatgcgc caggtgcaga tcgctgcggg tggtttaata
      781 ttaaacggcg ttgtactggg ttataccgta aatagcggtt tcttcttatt aagtggcttt
      841 gttggtgccg ggttactgtt tgcaggaatc agcggttttt gtggaatggc aaggttgtta
      901 gataagatgc cgtggaacca acgagcttga gaagcgacgc cggacgcgcc ctagcagcga
      961 catccggcct cagtaattag atcaggaaat cgtcgagaga tttaccttct gccagcgcct
     1021 gagcaattgg cttcggtgta cggccctgac cggtccaggt tttagtttca ccgttaacat
     1081 cagtgaattt atatttcgcc ggacgcggct ggcgtttttt accagcacgt ggtgcagcag
     1141 cagagctatt acccaataac tcttccgggt taattccgtc agctttcatc agctccagcc
     1201 aggtgctaat tttttcctga cgctcagcca gttcacgctg ctgctgttct tcttcttcac
     1261 gtctttcttt agtgacaacc ctgaattttt cgagcatttc ttcaagaacg tcaatggaga
     1321 attcgcgagc catcgcacgg agggtgcgaa tgttatttaa actttgtaac attacggaca
     1381 taataaagaa aaccttttaa cgccaaaaca aaaaagtatt tcgtcgcgta gtgtaagcga
     1441 tcacttatta tatttccaca aaatttaatc tatttacagt cctgcgcgag attatttcag
     1501 ctgttatttt ctgaaactct cagaataatt tcaagattat atattagcca ttttgatgag
     1561 taatttaggt tattggtgaa cagagtggcg ctctctgctc gctaactccg caagcctgac
     1621 aaaaccgaag tgaataaaaa tgcaatataa cagaataaaa gaaaacaccc ctcactggtg
     1681 ctttctgctg ttaatgtaca ttttctggca tcaaaaatgc tgtaaatgac ccggtcatga
     1741 caaacaccga attctctacg cttatagcgt tacctccccc ccaaactttc attagtgaaa
     1801 ttaaaggcaa gatcatatct aattgattta attgaaaata aatcatattg gcatgtaaaa
     1861 atccacaatg tacaaaaaac gagccgttac ggaatatttt atctacaaaa actaactaaa
     1921 taaaaatttt tcactaattg attagtcata gacagcgata tacgctatgc aaaaatgcag
     1981 atggcaatga gatccactgc tttcatctcc attaacatcc cattacgctt ttattaagga
     2041 gcattagcat gttctcaccg cagtcacgct tgcgtcatgc agttgcagat acgttcgcga
     2101 tggttgttta ctgttctgtc gtgaacatgt gtattgaagt tttcctctcc ggaatgagct
     2161 tcgaacagtc tttttattcc agattggtag cgattccggt gaacatctta attgcatggc
     2221 catacggtat gtaccgcgat ctgtttatgc gcgcggcacg caaagttagc ccgtcgggct
     2281 ggataaaaaa tctggctgat atcctggctt atgtgacgtt ccagtcaccg gtgtatgtgg
     2341 cgatcttgtt agtggtgggc gcagactggc atcagattat ggcggcggtc agttcaaaca
     2401 tcgttgtttc gatgttgatg ggggcggttt atggctactt cctcgattat tgccgccgac
     2461 tgtttaaagt cagccgttac cagcaggtaa aagcctgact cttcttttcg cgactggcat
     2521 cgccagtcgc gtctttaact ggcttcgcca aataaaccat tcaaataacg ttcaagcgca
     2581 acacgcgaac taaagccatg cgggataccg taatgctcgt gtcgctcgcc tgctaaatag
     2641 agcggaaatt gctggtaatg atcgcaagtt ttaatatcgg aagcgggttc agccagtgtt
     2701 gaactgcgtt cttttaatgt tggatgaatt accagagcgg tacgtcccat tcgcgcttcg
     2761 cgattaacat agacataatt ttccccacgg cggtaaccat acgctttctg ggttaccaca
     2821 tcgacagtaa aaccgacttt ttcaagtacg cgcgccacct cgtctggtcg taaatacata
     2881 tttgatcctc gttattttca accgcgggcc taccttacct gattgcgcat tcacaacgct
     2941 ttcagaaaag tccataaagc cgcgatgaac ctcagttaag aaaattatgg tctacactga
     3001 aaattacatc gaattctaat gggggatcat atgtttaacc gaccgaaccg caacgacgtt
     3061 gatgatggcg tgcaggatat ccagaatgat gtcaatcaat tagctgacag cctcgaatct
     3121 gtattgaagt cctggggcag cgacgccaaa ggggaagctg aagccgcacg cagtaaagct
     3181 caggcgttgc tgaaagaaac cagggcgcga atgcatggtc gtactcgcgt ccagcaagcc
     3241 gcgcgcgatg ccgttggctg agcggattct tttgttcgtg aaagaccctg gtgtagcgtg
     3301 ggtacagcag ctgcggtagg tatttttatc ggcgcactgt taagcatgcg caaatcgtag
     3361 tgcaaaaatg ataataaata cgcgtctttg accccgaagc ctgtcttcgg ggtttctttt
     3421 tgcctggtga atcacaaaaa tccccctccc ccgtcacgct catatccagg gtaatttcga
     3481 tcactatttg ctatatattg tgtagttgaa tcttttttca actacatcta gtatctctgt
     3541 atcaacagag agacaacccg acgcgtatca tcgcgccgta tcttcatttt aaacggaaat
     3601 acgaatcatg cgcataacta tttatactcg taacgattgc gttcagtgcc acgccaccaa
     3661 acgggcgatg gaaaaccggg gctttgattt tgaaatgatt aatgtcgatc gcgttcctga
     3721 agcagcggaa gcgttgcgtg ctcagggctt tcgtcagttg ccggtagtga ttgctggcga
     3781 tcttagctgg tctggtttcc gtccggacat gattaaccgt ctgcatccag cgccacacgc
     3841 ggccagtgca tgagccagct cgtctacttc tccagcagct ccgaaaacac gcagcgtttt
     3901 atcgaacgtt taggtctgcc cgcggtgcgc atcccgctca atgagcggga acggattcag
     3961 gtagacgagc cttacatcct gatcgtgccc tcttacggcg gcggcggtac ggctggcgcg
     4021 gtgccacgac aggtaattcg ctttttaaac gacgagcaca accgggcgtt gcttcgcggc
     4081 gttattgctt ctggtaatcg caactttggt gaggcgtatg gccgcgccgg agatgtgatt
     4141 gcccggaaat gcggcgtgcc gtggctgtac cgttttgaac tcatgggtac gcaaagcgat
     4201 atcgaaaacg ttcgtaaagg agtaaccgaa ttttggcaac gacaaccgca gaatgcctga
     4261 cgcaggaaac gatggattac cacgcgctga atgcgatgct taatctttac gatagcgcag
     4321 gtcgcattca gttcgataaa gaccgccagg ccgttgacgc ctttattgcg acgcatgtgc
     4381 gtccgaacag tgtgaccttc agtagccagc agcagcgcct gaactggctg gtcaacgaag
     4441 gttactatga tgaaagcgtt cttaatcgct actctcgcga ctttgtcatt acgctgtttg
     4501 cccacgcaca caccagcggt tttcgtttcc agacattcct cggggcatgg aagttttaca
     4561 ccagctatac gttgaagaca ttcgacggta aacgttatct ggaagatttt gccgatcgag
     4621 tcacgatggt ggcgctgacg ctggcacaag gcgatgagac gctggcgttg caactgaccg
     4681 atgaaatgct gtcaggacgc tttcagccag ccacgccaac attcctcaac tgcggtaagc
     4741 agcagcgcgg cgaactggtt tcctgttttt tgctgcgtat tgaagacaat atggagtcga
     4801 ttggtcgggc ggtaaattcc gcactgcagc tgtcgaaacg cggcggcggc gtagcatttt
     4861 tgctgtcgaa tctgcgggaa gcgggcgcgc caattaaacg tattgaaaat caatcttctg
     4921 gcgtaattcc ggtgatgaaa atgttggaag acgcattttc ctatgccaac caactcggcg
     4981 cgcgtcaggg ggcaggtgca gtctatttac atgctcatca tcccgatatt ctgcgttttc
     5041 tcgacacgaa acgggaaaat gccgacgaaa aaatccgcat taaaacactg tcgctgggcg
     5101 tggtgatccc ggatatcact ttccatctgg caaaagagaa tgcgcagatg gcgctgtttt
     5161 cgccctatga cgttgagcga gtttatggca agccgtttgc cgatatcgcc attagcgaac
     5221 actatgacga actggttgcc gatgaacgca tccgcaaaaa atacctcaac gcccgtgatt
     5281 tcttccagcg actggcagaa atccagtttg agtccggcta tccctacatc atgtatgaag
     5341 acacagtaaa ccgtgctaac cctatcgccg ggcgcataaa tatgagtaat ctctgctcag
     5401 aaattttgca ggttaacagc gcctcagagt atgacgagaa tctcgactat gcccgcacag
     5461 gccatgatat ttcctgcaat ttaggttcgt tgaatattgc gcacaccatg gattcccccg
     5521 atttttcccg cacggtagag actgccgtgc gcggtttaac ggcggtgtca gatatgagtc
     5581 atatccgcag cgtgccgtcc atcgaagccg gaaatgccgc ctcgcacgcc atcggactgg
     5641 ggcagatgaa tttacatggt tatctggcga gggaaggcat cgcttatggt tcgccggaag
     5701 ccctggattt caccaatctc tatttctata ccatcacctg gcacgcactg cgtacctcga
     5761 tgttgctggc gcgcgaacgc ggtgaaacct tcgccgggtt caaacagtca cgctatgcca
     5821 gtggtgaata tttcagccaa tatctgcaag gtaactggca gccgaaaacg gcgaaagttg
     5881 gcgaactgtt tgcccgcagc tgtattacgt tacctacccg tgagatgtgg gcgcaactgc
     5941 gcgacgacgt gatgcgctac ggcatataca accagaatct ccaggcggtg ccgccaaccg
     6001 ggtctatctc ttatatcaac catgctacgt cgagtattca tccgattgtg gcgaaagtag
     6061 agatacgcaa agagggcaaa acaggacgcg tttactaccc tgccccgttt atgactaacg
     6121 aaaatctggc gctgtatcag gacgcttacg aaattggcgc agaaaagatc atcgacacct
     6181 acgcggaagc gactcgccat gtcgatcagg ggctgtcgct gacgctgttt ttccccgata
     6241 ccgccaccac tcgcgatatc aacaaagcgc agatttacgc ctggcgcaaa ggtatcaaaa
     6301 cgctctatta catccgcctg cgtcagatgg cgctggaagg cactgaaatt gaaggctgcg
     6361 tctcctgtgc actttaagga atatctatga aactctcacg tatcagcgcc atcaactgga
     6421 acaagatatc tgacgataaa gatctggagg tgtggaatcg cctgaccagc aatttctggc
     6481 taccagaaaa ggtgccgctg tcgaacgata ttcccgcctg gcagacatta actgtcgtag
     6541 aacaacaact gacgatgcgc gtttttactg gcctgacgct gctcgacacg ctgcaaaatg
     6601 ttatcggcgc gccttcgctg atgcccgatg cgctcacgcc tcatgaagag gcggtattat
     6661 cgaatatcag ctttatggaa gcggttcatg cccgctctta cagttcgatt ttctcgacgc
     6721 tgtgccagac caaagatgtc gatgccgcct acgcctggag tgaagaaaac gcaccgttgc
     6781 agcgaaaagc tcagattatt cagcaacatt atcgcggtga tgatccgctg aaaaagaaaa
     6841 tcgccagtgt gtttcttgaa tcttttttgt tctattccgg tttctggctg ccgatgtatt
     6901 tttccagccg cggaaagctg accaataccg cggacctgat ccgtctgatt atccgcgatg
     6961 aagcagtcca cggttactac ataggctata aatatcagaa aaacatggaa aagatatctc
     7021 tgggacaacg tgaagagttg aagagtttcg ccttcgattt gttgctggaa ctctacgaca
     7081 acgagttgca atacaccgat gagctgtacg ccgaaacccc gtgggctgac gatgtgaaag
     7141 cgtttctctg ttacaacacc aataaggctt tgatgaatct gggctacgaa ccgttatttc
     7201 ccgcagaaat ggcggaagtg aatccggcaa tcctcgccgc gctttcgccg aatgccgatg
     7261 aaaatcacga tttcttttcc ggttcaggct cctcttatgt gatggggaaa gcggttgaaa
     7321 cagaagatga agactggaat ttctgagggt gttattttca aaaatatcac tacccgcagc
     7381 agggaaataa ttcccgccaa atagcttttt atcacgcaaa tattctgtag tgatctacac
     7441 tgatactctg ttgcattatt cgcctgaaac tacaatattc aggcgttttt tcgctatcta
     7501 tgacaaaaaa tatcaacttt tctcgatttg ctctcagccc ttatatcacg gaaaattccg
     7561 gcgatttgct cgcatcaata ttcatgccac atttgccctc aggggttgcc tcagattctc
     7621 agtatgttag ggtagaaaaa agtgactatt cccattgggt aatatatcga cataaacaaa
     7681 taaagaaatc attctattgc atggccatta aattaaaaat taaaaatctt tataaaatat
     7741 ttggtgagca tccacagcga gcgttcaaat atatcgaaca aggactttca aaagaacaaa
     7801 ttctggaaaa aactgggcta tcgcttggcg taaaagacgc cagtctggcc attgaagaag
     7861 gcgagatatt tgtcatcatg ggattatccg gctcgggtaa atccacaatg gtacgccttc
     7921 tcaatcgcct gattgaaccc acccgcgggc aagtgctgat tgatggtgtg gatattgcca
     7981 aaatatccga cgccgaactc cgtgaggtgc gcagaaaaaa gattgcgatg gtcttccagt
     8041 cctttgcctt aatgccgcat atgaccgtgc tggacaatac tgcgttcggt atggaattgg
     8101 ccggaattaa tgccgaagaa cgccgggaaa aagcccttga tgcactgcgt caggtcgggc
     8161 tggaaaatta tgcccacagc tacccggatg aactctctgg cgggatgcgt caacgtgtgg
     8221 gattagcccg cgcgttagcg attaatccgg atatattatt aatggacgaa gccttctcgg
     8281 cgctcgatcc attaattcgc accgagatgc aggatgagct ggtaaaatta caggcgaaac
     8341 atcagcgcac cattgtcttt atttcccacg atcttgatga agccatgcgt attggcgacc
     8401 gaattgccat tatgcaaaat ggtgaagtgg tacaggtcgg cacaccggat gaaattctca
     8461 ataatccggc gaatgattat gtccgtacct tcttccgtgg cgttgatatt agtcaggtgt
     8521 tcagtgcgaa agatattgcc cgccggacac cgaatggctt aattcgtaaa actcctggct
     8581 tcggcccacg ttcggcactg aaattattgc aggatgaaga tcgcgaatat ggctacgtta
     8641 tcgaacgcgg taataagttt gtcggcgcag tctccatcga ttcgcttaaa accgcgttaa
     8701 cgcagcagca aggtcttgat gcggcgctga ttgatgcgcc gttagcagtc gatgcacaaa
     8761 cgcctcttag cgagttgctc tctcatgtcg gacaggcacc ctgtgcggtg cccgtggtcg
     8821 acgaggacca acagtatgtc ggcatcattt cgaaaggaat gctgctgcgc gctttagatc
     8881 gtgagggggt aaataatggc tgatcaaaat aatccgtggg ataccacgcc agcggcggac
     8941 agtgccgcgc aatccgcaga cgcctggggt acaccgacga ctgcaccggc tgacggcggt
     9001 ggtgctgact ggctgaccag tacgcctgcg ccaaacgtcg agcattttaa tattctcgat
     9061 ccgttccata aaacgctgat cccgctcgac agttgggtca ctgaagggat cgactgggtt
     9121 gttacccatt tccgtcccgt cttccagggc gtgcgcgttc cggttgatta tatcctcaac
     9181 ggtttccagc aattgctgct gggtatgcca gcgccagtgg cgattatcgt tttcgctctc
     9241 atcacctggc agatttccgg ggtcggaatg ggcgtggcga cgctggtctc gctgattgcc
     9301 atcggcgcaa tcggtgcctg gtcgcaggca atggtgactc tggcgctggt gttaactgcc
     9361 ctgctgttct gtatcgtcat cggtttgccg ttggggatat ggctggcgag aagtccgcga
     9421 gcggcgaaaa ttattcgtcc actgcttgat gccatgcaga ccacgccagc gtttgtttat
     9481 ctggtgccaa tcgtcatgct atttggtatc ggtaacgtgc cgggcgtggt ggtgacgatc
     9541 atctttgcgc tgccgccgat tatccgtctg accattctgg ggattaacca ggttccggcg
     9601 gatctgattg aagcctcgcg ctcattcggt gccagcccgc gccagatgct gttcaaagtt
     9661 cagttaccgc tggcgatgcc gaccattatg gcgggcgtta accagacgct gatgctggcc
     9721 ctttctatgg tggtcattgc ctcgatgatt gccgtcggcg ggctgggtca gatggtactt
     9781 cgcggtatcg gtcgtctgga tatggggctt gccaccgttg gcggcgtcgg gattgtgatc
     9841 ctcgccatta tcctcgatcg cctgacgcag gccgttgggc gcgactcacg cagtcgcggc
     9901 aaccgtcgct ggtacaccac tggccctgtt ggtctgctga cccgcccatt cattaagtaa
     9961 ctctgcactt gcccggtgac gccgggcatt atcaccctgc caaaaaaagg aataacaatg
    10021 cgacatagcg tactttttgc gacagcgttt gccacgctta tctctacaca aacttttgct
    10081 gccgatctgc cgggcaaagg cattactgtt aatccagttc agagcaccat cactgaagaa
    10141 accttccaga cgctgctggt cagtcgtgcg ctggagaaat taggttatac cgtcaacaaa
    10201 cccagcgaag tggattacaa cgttggctac acctcgcttg cttccggcga tgcaaccttc
    10261 accgccgtga actggacgcc actccatgac aacatgtacg aagctgccgg tggcgataag
    10321 aaattttatc gtgaaggggt atttgttaac ggcgcagcac agggttacct gatcgataag
    10381 aaaaccgccg accaatacaa aatcaccaac atcgcacaac tgaaagatcc gaagatcgcc
    10441 aaactgttcg ataccaacgg cgacggtaaa gcggatttaa ccggttgtaa ccctggctgg
    10501 ggctgcgaag gtgcgatcaa ccaccagctt gccgcgtatg aactgaccaa caccgtgacg
    10561 cataatcagg ggaactacgc agcgatgatg gccgacacca tcagtcgcta caaagagggc
    10621 aaaccggtgt tttattacac ctggacgccg tactgggtga gtaatgagct gaagccaggg
    10681 aaagatgtgg tctggttgca ggtgccgttc tccgcactgc cgggcgataa aaatgccgat
    10741 accaaactgc cgaatggcgc gaattacggc ttcccggtca gcaccatgca tatcgttgcc
    10801 aacaaagcct ggaccgagaa aaatccggcg gcagccaaac tgtttgccat tatgcagttg
    10861 ccagtggccg atattaacgc ccagaacgcc attatgcatg acggcaaagc ctcagaaggc
    10921 gatattcagg gccacgttga tggctggatc aaagcccacc agcagcagtt cgatggctgg
    10981 gtgaatgagg cgctggcagc gcagaagtaa tttttattcg ggcggataag gcgttttcgc
    11041 ctcatccgcc gctctgtaca caatgcctga tgcgacgctg acgcgtccta tcatgcctac
    11101 aaaccacatc gcacatttca acaagctatc tttcatccca tattcatcaa catccgctat
    11161 tattgatttc cagcttaatc atcacctgat gaacaaaaat aatgactaaa cctaatcatg
    11221 agcttagccc ggcgctgatc gtgctgatgt ctatcgccac cggtctggcg gtagccagta
    11281 actattacgc ccagccattg ctcgacacca tcgcgcgtaa cttttccctt tccgccagtt
    11341 cggcaggctt tattgttacc gccgcgcagt tgggctatgc cgcaggtcta ctgtttcttg
    11401 ttcccctcgg tgatatgttt gaacgccgcc gcctgattgt ctcgatgacc ttactggcag
    11461 cgggcggtat gttaatcact gccagcagtc agtcactggc gatgatgatc ctcggtacgg
    11521 cattaaccgg tttattctca gtcgtggcac aaattctggt tccgctggca gcgacgctgg
    11581 cttcaccgga taaacgcggc aaagtggttg gcaccattat gagcggtctg ctgttgggga
    11641 tattgctggc acgaaccgtt gccgggttac tggcaaacct cggcggctgg cgcaccgtct
    11701 tttgggttgc ttcggtgtta atggcactga tggcgctggc attatggcgt ggtctgccac
    11761 aaatgaaatc agaaacccac ctcaactacc cacagttgtt gggttccgtt ttcagcatgt
    11821 tcatcagcga taagatcctg cgcacccgcg cgttgctggg ctgcatgacc tttgccaact
    11881 tcagcattct ctggacctca atggcctttt tgcttgccgc tccacctttt aactacagcg
    11941 atggtgacat tggtctgttt gggcttgcgg gagctgccgg agcgttgggc gctcgtccgg
    12001 cgggcggttt tgccgacaag ggcaaatcgc accacaactt tcggtctgct tctgctgtta
    12061 ctttcatggc tggcaatctg gtttggtcac acttccgtac tggcgttgat tatcggcatt
    12121 ctggtgctgg acctcaccgt acagggcgtg catatcacta accagacggt aatttatcga
    12181 atacatcctg atgcgcgtaa tcgcctgacc gcaggttaca tgaccagcta ctttattggc
    12241 ggtgccgccg gttcgctaat ttcagcctca gcctggcaac atggcggttg ggctggcgtt
    12301 tgtctggctg gcgcgacgat tgccctggtt aacttactgg tctggtggcg aggttttcat
    12361 cgtcaggaag ccgcaaatta agcaatcagc aaccgttttc gtggctttac acttataagg
    12421 gtgttaagaa gcccatcagt ctgataaggt taagatattc attcagtcta tttataatat
    12481 taacaatcgt taagcgtaca ctctatggaa agccctactc cacagcctgc tcctggttcg
    12541 gcgaccttca tggaaggatg caaagacagt ttaccgattg ttattagtta tattccggtg
    12601 gcctttgcgt tcggtctgaa tgcgacccgt ctgggattct ctcctctcga aagcgttttt
    12661 ttctcctgca tcatttatgc aggcgcgagc cagttcgtca ttaccgcgat gctggcagcc
    12721 gggagtagtt tgtgggttgc tgcactgagc gtcatggcaa tggatgttcg ccatgttttg
    12781 tatggcccgt cactgcgtag ccgtattatt cagcgtctgc aaaaatcgaa aaccgcccta
    12841 tgggcgtttg gcctgacgga tgaggttttt gccgccgcca ccgcaaaact ggtacgcaat
    12901 aatcgccgct ggagcgagaa ctggatgatc ggcattgcct tcagttcatg gtcatcgtgg
    12961 gtatttggta cggtaatagg ggcattctcc ggcagcggct tgctgcaagg ttatcccgcc
    13021 gttgaagctg cattaggttt tatgcttccg gcactcttta tgagtttcct gctcgcctct
    13081 ttccagcgca aacaatctct ttgcgttacc gcagctttag ttggtgccct tgcaggcgta
    13141 acgctatttt ctattcccgt cgccattctg gcaggcattg tctgtggctg cctcactgcg
    13201 ttaatccagg cattctggca aggagcgccc gatgagctat gaggttctgc tgcttgggtt
    13261 actggttggc gcggcgaatt attgcttccg ctatttgccg ctgcgcctgc gtgtgggtaa
    13321 tgcccgccca accaaacgtg gcgcggtagg tattttgctc gacaccattg gcatcgcctc
    13381 gatatgcgct ctgctggttg tctctaccgc accagaagtg atgcacgata cacgccgttt
    13441 cgtgcccacg ctggtcggct tcgcggtact gggtgccagt ttctataaaa cacgcagcat
    13501 tatcatccca acactgctta gtgcgctggc ctatgggctc gcctggaaag tgatggcgat
    13561 tatataaccc acaagaatca tttttctaaa acaatacatt tactttattt gtcactgtcg
    13621 ttactatatc ggctgaaatt aatgaggtca tacccaaatg gatagttcgt ttacgcccat
    13681 tgaacaaatg ctaaaatttc gcgccagccg ccacgaagat tttccttatc aggagatcct
    13741 tctgactcgt ctttgcatgc acatgcaaag caagctgctg gagaaccgca ataaaatgct
    13801 gaaggctcag gggattaacg agacgttgtt tatggcgttg attacgctgg agtctcagga
    13861 aaaccacagt attcagcctt ctgaattaag ttgtgctctt ggatcatccc gtaccaacgc
    13921 gacgcgtatt gccgatgaac tggaaaaacg cggttggatc gaacgtcgta aaagcgataa
    13981 cgatcgccgc tgcctgcatc tgcaattaac ggaaaaaggt cacgagtttt tgcgcgaggt
    14041 tttaccaccg cagcataact gcctgcatca actctggtcc gcgctcagca caacagaaaa
    14101 agatcagctc gagcaaatca cccgcaaatt gctctcccgt ctcgaccaga tggaacaaga
    14161 cggtgtggtt ctcgaagcga tgagctaacg cgtcatctcg ctcaaaaatc cagatttata
    14221 aaagaaaaaa tgaccggcca gcatcgcaac atgctggcct ttttggcaag caggtcggct
    14281 cagccgatga gttaagaaga tcgtggagaa caatatgagc gcaaatgcgg agactcaaac
    14341 cccgcagcaa ccggtaaaga agagcggcaa acgtaagcgt ctgctcctcc ttctcacctt
    14401 gctctttata attattgccg tagcgatagg gatttattgg tttttggtac tgcgtcattt
    14461 cgaagaaacc gatgacgcat acgtggcagg gaatcagatt caaattatgt ctcaggtgtc
    14521 tggcagcgtg acgaaagtct gggccgataa caccgatttt gtaaaagaag gcgatgttct
    14581 ggtcactctc gacccgacag atgctcgcca ggcgtttgaa aaagccaaaa ctgcactggc
    14641 ttccagcgtt cgccaaaccc accagctgat gattaacagc aaacagttgc aggcgaatat
    14701 tgaggtgcaa aaaatcgccc tcgcgaaagc acaaagcgac tacaaccgcc gtgtgccgct
    14761 gggcaatgcc aacctgattg gtcgcgaaga gctgcaacac gcccgcgacg ccgtcaccag
    14821 tgcccaggcg caactggacg tcgctattca acaatacaat gccaatcagg cgatgattct
    14881 ggggactaaa ctggaagatc agcctgccgt gcaacaggct gccaccgaag tacgtaacgc
    14941 ctagctggcg ctggagcgta cccgtattgt cagtccgatg accggttatg tttcccgtcg
    15001 tgccgtgcaa ccgggtgcgc aaattagccc aacgacgccg ctgatggcgg tcgttccagc
    15061 caccaatatg tgggtggatg ccaactttaa agagacgcag attgccaata tgcgcatcgg
    15121 tcagccggtc accatcacca ccgatattta cggcgatgat gtgaaataca ccggtaaagt
    15181 ggttggtctg gatatgggca caggtagcgc gttctcactg cttccggcgc aaaatgcgac
    15241 cggtaactgg atcaaagtcg ttcagcgtct acctgtgcgt atcgaactgg accagaaaca
    15301 gctggagcaa tacccgctgc gcattggttt gtccacgctg gtgagcgtca ataccactaa
    15361 ccgtgacggt caggtactgg caaataaagt acgttcaaca ccggtcgcgg taagtaccgc
    15421 gcgtgaaatc agcctggcac ctgtcaataa actgatcgac gatatcgtaa aagctaacgc
    15481 tggctaatcc agaggtgcgt gtgatgcaac agcaaaaacc gctggaaggc gcgcaactgg
    15541 tcattatgac gattgcgctg tcactggcga cattcatgca ggtgctgaac tccaccattg
    15601 ctaacgtggc gatccccact atcgccggga atctgggctc atcgctcagc caaggaacgt
    15661 gggtaatcac ttctttcggg gtggcgaatg ccatctcgat cccgcttacc ggctggctgg
    15721 caaagcgcgt cggggaagtg aaactgttcc tttggtccac catcgccttt gctattgcgt
    15781 cttgggcgtg tggtgtctcc agcagcctga atatgctgat cttcttccgc gtgattcagg
    15841 ggattgtcgc cgggccgttg atcccgcttt cgcaaagtct attgctgaat aactacccgc
    15901 cagccaaacg ctcgatcgcg ctggcgttgt ggtcgatgac ggtgattgtc gcgccaattt
    15961 gcggcccgat cctcggcggt tatatcagcg ataattacca ctggggctgg atattcttca
    16021 tcaacgtgcc gattggcgtg gcggtggtgt tgatgacact gcaaaccctg cgcggacgtg
    16081 aaacccgcac cgaacggcgg cggattgatg ccgtggggct ggcactgctg gttattggta
    16141 tcggcagcct gcagattatg ctcgaccgcg gtaaagagct ggactgtttt tcatcacagg
    16201 aaattatcat tcttaccgtg gtggcggtgg tggctatctg cttcctgatt gtctgggagc
    16261 tgaccgacga taacccgata gtcgatctgt cgttgtttaa gtcgcgcaac ttcaccatcg
    16321 gctgcttgtg tatcagcctc gcgtatatgc tctacttcgg cgctattgtt ctgctgccgc
    16381 agttgttgca ggaggtctac ggttacacgg cgacctgggc aggtttggcc tctgcgccgg
    16441 tagggattat tccggtgatt ctgtcgccga ttatcggccg cttcgcgcat aaactggata
    16501 tgcggcggct ggtaaccttc agctttatta tgtatgccgt ctgcttctac tggcgtgcct
    16561 atacctttga accaggtatg gatttttgcg cgtcggcctg gccgcagttt atccaggggt
    16621 ttgcggtggt ctgcttcttt atgccgctga ccaccattac gctgtctggt ttgccaccgg
    16681 aacgactggc ggcggcatcg agcctctcta actttacgcg aacgctggcg gggtctatcg
    16741 gcacgtcgat aaccacgacc atgtggacca accgcgagtc gatgcaccat gcgcagttga
    16801 ctgagtcggt aaacccgttc aacccgaatg cccaggcgat gtacagtcaa ctggaagggc
    16861 ttgggatgac gcaacagcag gcatcaggct ggattgccca gcagatcacc aatcaggggc
    16921 tgattatttc cgccaatgag atcttctgga tgtcagccgg gatattcctc gtactgctgg
    16981 ggctggtgtg gtttgctaaa ccgccatttg gcgcaggtgg cggcggaggc ggtgcgcact
    17041 aagtacaact aagccagttc atttgaactg gcttttttca attaattgtg aagatagttt
    17101 actgactaga tgtgcagttc ctgcaacttc tctttcggca gtgccagttc ttcgttgctg
    17161 ttgatgcgta cctcacgttc cagaatgcta cgcgcaatat cctgcgcttc ctgcaacgag
    17221 tgcatctggt aagtgccaca ctggtagacg ttcagttccg ggatctgatt ctgatcctgc
    17281 actttcagca cgtcttccat tgccgctttc caggcatcag caacacgctg ctcatctggc
    17341 gtaccaatca gactcatata aaaaccggtg cggcagccca ttggcgagat atcgataatc
    17401 tctacaccat taccgttaag atggttacgc ataaaaccag caaacaggtg ctccagggta
    17461 tggatccctc tttctggcat cacttctttg ttcggcacgc agaagcgcag atcgaacacg
    17521 gtgattgcgt cgccatgcgg ggtgttcatt gttttcgcca cccgaactgc aggcgcttcc
    17581 atccgggtat gatcgactgt gaagctatct aacaacggca tttagccacc tccggtaatt
    17641 tttttaaaaa ttttctgaac tctttcttcc caggcgagtc tgagtatatg aaagacgcgc
    17701 atttgttatc atcatccctg aattcagaga tgaaattttg gccactcccg agtggccttt
    17761 ttcttttctg tcaggcgtgt ttttccagcc acaccgcaaa cggttcggta tcagcggttt
    17821 ccatttcctg ctgacggcgt tcagatgcct cgcgctcggc tacaaaatcc tcttcgcgca
    17881 gaatttccag cggctcttca cgcagcagat tacggtaggc ttctgcaaac gctttgcctg
    17941 ttccgccaat accagtatca atcatagacc ttaagatacg ggcagagaaa gtcagatcgg
    18001 gattatcgaa gcaggcaacc agttcatcac acactttctg atacgcttcg ccgccgttaa
    18061 tgctatccag cgtttgcgcg acgcgtttca gatcgcggaa cagatcttta cccacctgca
    18121 gtaacgggaa ctgcgcggtt tcgcagccga tacccagcgt caggcccggt ttgcgacctt
    18181 caaggatcac ccggttccag ttaacgcgcg tacaggcaag ttcgctactg ctcatttccg
    18241 gtgcatcagc cagcgcacac cagaccataa acaggtcgag gaatcgcacc tgctgttcat
    18301 ctacaccaat cggcgagaac gggttgatgt ccagcgaacg cacttcaata tattcaatgc
    18361 cgccacgtaa cagcgcatca gaaggtgact cgccgctgcg ggtaacgcgt tttggacgaa
    18421 tcggcgcata cagttcgttt tcaatctgca gcacgttgct attgatttgc agcctcttac
    18481 cgtctttctc aataccaatc ttcgcgtact cttccgatgg cgttttgatt gcctgtttaa
    18541 ggcccgctac gtactcgtaa agatcgttga aggtaatacc aagattgctt tgcgatttat
    18601 tggtatagcc gagatcgctc aaacgaagag aggtcgcata cggcaggtaa tacataccgc
    18661 actcggtttt ctcaaacggc agcgacgttg gttttccttg caggaaagaa gaacaaatcg
    18721 ccggagatgc accaaacaga taaggaataa cccaaccgaa acgatagtaa ttgcggataa
    18781 cgcggaaata gcccgcagaa attttctctt tggcatcagc gcccgagata tcaccgcact
    18841 tcgcttgcca gaatgccatt ggcaaagaga aattgtagtg cacgccggaa atggtttgca
    18901 tcagcgcgcc gtagcgattt ttcagccctt cacgatacag cgttttaaag cgtccggtgt
    18961 tagaagtgcc gtactgtgcc agttcgatgt cctgaccttc tgcgatgtag catggcatac
    19021 ttaacggcca catccgctca tcgcccatat tgcgcgccgt ataacgatgc agatcgcgca
    19081 taaaggtcag catatgttca atatcaccat ccactggtgt aatgaattcc agcaatgctt
    19141 ccgcaaaatc ggtagtaatc catttgtgcg tcagtgcgga acctaatgct tcaggatgac
    19201 ctgttgttgc cagtgtgcca tcagcattaa cacgcaaagt ttcgcgctcc agcccacgct
    19261 gtatcccctt taacgcctga ggatgttttt ccagccaggc cagcgcctgt gatacgtccg
    19321 ggatcaaatt gacctcccgc ctgtcaaaat cgttttaatt agcataactg taatggtgac
    19381 catatgtgca ggcctacaat tagtgccacc acatcatgcc ctgaacggtc gctgctgcaa
    19441 ctgcaacata gcgtaacgct ttaccaaggc acaaaaaaaa gattactggc ccccaggaga
    19501 tgcgcatcca tcccgctaac agacacagta aatcgccaac cacgggcatc cagcttaata
    19561 atagcgtgac tgcaccatag cgtttcagcc agccggtagc tttctcttgc cagcgcgatg
    19621 ttttacgcaa tggaaagaaa cgcccaagga taacgttagt taaccctcca aggctattac
    19681 ccattgttgc tgttaaaact aaaacccagg gatgactgat cccggaaagc aacattgcca
    19741 ccagcacgac ttcggagttg ccgggtaata gtgtagcgct gagaaaacta ctggcaaaca
    19801 acgagaaaag cgataacgct tcactcacag caagcgaaca tccacagcgt ccatgcctgc
    19861 tgcacgggcc gcctgaatac cgaaatcggc atcttcaaag accacacact gcgtcggttg
    19921 cacgcccata cgctgcgcgc acaacaaaaa tgtgtctggc gcgggtttat ggtgtttgac
    19981 gtgatcagca gcgacgacgg cgtcaaaata acggcgtaat cccaggtgcg ccagcaatgc
    20041 ctcagcgatg gcgctttcac tccccgttcc tacagccatt gggcgacgac catgccaact
    20101 tttcaccact tcaacaagag gaagcggctc gacactatcc agcagcatac ttcttactgc
    20161 ttctgttttt tcacgcgcta acgcatgcgg gtcgagatcg gcctgattca gctcaataat
    20221 tgcctgagca atacgccagg tgggcgatcc attaagcgca atcatcgcct gaatatcgta
    20281 ctgaagaccg tagtgcccta atacttcgcg ccacgcttta cggtgcgtag gcgccgtatc
    20341 caggattgtg ccatccatat caaaaattaa acctgcataa cgctcgtaca tggtcttctc
    20401 gcaagtcgaa aaatatgacg ctactttagc gtaattgctt gattttgtcg ctgatggcga
    20461 aggagaagaa aattcaggaa atggataaag tagttattta gaaatgagat atttttgaag
    20521 gaaatttttt ggagaatatg gtgcatccgg gaggattcga acctccgacc gctcggttcg
    20581 tagccgagta ctctatccag ctgagctacg gatacatcgg gaaacttact ttactgcaga
    20641 ttttttgata ccgctactaa agccgtatca agtaag
//
LOCUS       DAJTRC010000069        20615 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.069, whole genome shotgun sequence.
ACCESSION   DAJTRC010000069 DAJTRC010000000
VERSION     DAJTRC010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20615)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20615)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20615
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.069"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(77..4537)
                     /gene="mukB"
                     /locus_tag="OO357_002709"
     CDS             complement(77..4537)
                     /gene="mukB"
                     /locus_tag="OO357_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukB"
                     /protein_id="HCR5887603.1"
                     /translation="MIERGKFRSLTLINWNGFFARTFDLDELVTTLSGGNGAGKSTTM
                     AAFVTALIPDLTLLHFRNTTEAGATSGSRDKGLHGKLKAGVCYSMLDTINSRHQRVVV
                     GVRLQQVAGRDRKVDIKPFAIQGLPMSVQPTQLVTETLNERQARVLPLNELKDKLEAM
                     EGVQFKQFNSITDYHSLMFDLGIIARRLRSASDRSKFYRLIEASLYGGISSAITRSLR
                     DYLLPENSGVRKAFQDMEAALRENRMTLEAIRVTQSDRDLFKHLISEATNYVAADYMR
                     HANERRVHLDKALEFRRELHTSRQQLAAEQYKHVDMARELAEHNGAEGDLEADYQAAS
                     DHLNLVQTALRQQEKIERYEVDLDELQIRLEEQNEVVAEAIERQEENEARAEAAELEV
                     DELKSQLADYQQALDVQQTRAIQYNQAIAALNRAKELCHLPDLTADSAAEWLETFQAK
                     ELEATEKMLSLEQKMSMAQTAHSQFEQAYQLVVAINGPLARNEAWDVARELLREGVDQ
                     RHLAEQVQPLRMRLSELEQRLREQQEAERLLADFCKRQGKNFDIDELEALHQELEARI
                     ASLSDSVSNAREERMALRQEQEQLQSRIQSLMQRAPVWLAAQNSLNQLSEQCGEEFSS
                     SQDVTEYLQQLLEREREAIVERDEVGARKNAVDEEIERLSQPGGSEDQRLNALAERFG
                     GVLLSEIYDDVSLEDAPYFSALYGPSRHAIVVPDLSQVTEHLEGLTDCPEDLYLIEGD
                     PQSFDDSVFSVDELEKAVVVKIADRQWRYSRFPEVPLFGRAARESRIESLHAEREVLS
                     ERFATLSFDVQKTQRLHQAFSRFIGSHLAVAFESDPEAEIRQLNSRRVELERALSNHE
                     NDNQQQRIQFEQAKEGVTALNRILPRLNLLADDSLADRVDEIRERLDEAQEAARFVQQ
                     FGNQLAKLEPIVSVLQSDPEQFEQLKEDYAYSQQMQRDARQQAFALTEVVQRRAHFSY
                     SDSAEMLSGNSDLNEKLRERLEQAEAERTRAREALRGHAAQLSQYNQVLASLKSSYDT
                     KKELLNDLQRELQDIGVRADSGAEERARIRRDELHAQLSNNRSRRNQLEKALTFCEAE
                     MDNLTRKLRKLERDYFEMREQVVTAKAGWCAVMRMVKDNGVERRLHRRELAYLSADDL
                     RSMSDKALGALRLAVADNEHLRDVLRMSEDPKRPERKIQFFVAVYQHLRERIRQDIIR
                     TDDPVEAIEQMEIELSRLTEELTSREQKLAISSRSVANIIRKTIQREQNRIRMLNQGL
                     QNVSFGQVNSVRLNVNVRETHAMLLDVLSEQHEQHQDLFNSNRLTFSEALAKLYQRLN
                     PQIDMGQRTPQTIGEELLDYRNYLEMEVEVNRGSDGWLRAESGALSTGEAIGTGMSIL
                     VMVVQSWEDESRRLRGKDISPCRLLFLDEAARLDARSIATLFELCERLQMQLIIAAPE
                     NISPEKGTTYKLVRKVFQNTEHVHVVGLRGFAPQLPETLLGRDEAPSQAS"
     gene            complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="OO357_002710"
     CDS             complement(4537..5241)
                     /gene="mukE"
                     /locus_tag="OO357_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukE"
                     /protein_id="HCR5887604.1"
                     /translation="MSSTNIEQVMPVKLAQALANPLFPALDSALRSGRHIGLDELDNH
                     AFLMDFQEYLEEFYARYNVELIRAPEGFFYLRPRSTTLIPRSVLSELDMMVGKILCYL
                     YLSPERLANEGIFTQQELYDELLTLADEAKLLKLVNNRSTGSDVDRQKLQEKVRSSLN
                     RLRRLGMVWFMGHDSSKFRITESVFRFGADVRAGDDPREAQRRLIRDGEAMPIENHLQ
                     LNDETEESQPDSGEEE"
     gene            complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="OO357_002711"
     CDS             complement(5222..6544)
                     /gene="mukF"
                     /locus_tag="OO357_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome partition protein MukF"
                     /protein_id="HCR5887605.1"
                     /translation="MSEFSQTVPELVAWARKNDFSISLPVDRLSFLLAVATLNGERLD
                     GEMSEGELVDAFRHVSDAFEQTSETIGVRANNAINDMVRQRLLNRFTSEQAEGNAIYR
                     LTPLGIGITDYYIRQREFSTLRLSMQLSIVAGELKRAADAAEEGGDEFHWHRNVYAPL
                     KYSVAEIFDSIDLTQRLMDEQQQQVKDDIAQLLNKDWRAAISSCELLLSETSGTLREL
                     QDTLEAAGDKLQANLLRIQDATMTHDDLHFVDRLVFDLQSKLDRIISWGQQSIDLWIG
                     YDRHVHKFIRTAIDMDKNRVFAQRLRQSVQTYFDEPWALTYANADRLLDMRDEEMALR
                     DEEVTGELPEDLEYEEFNEIREQLAAIIEEQLAVYKTRQVPLDLGLVVREYLSQYPRA
                     RHFDVARIVIDQAVRLGVAQADFTGLPAKWQPINDYGAKVQAHVIDKY"
     gene            complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="OO357_002712"
     CDS             complement(6541..7326)
                     /gene="cmoM"
                     /locus_tag="OO357_002712"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706839.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine 5-oxyacetic acid(34)
                     methyltransferase CmoM"
                     /protein_id="HCR5887606.1"
                     /translation="MQDRNFDDIAEKFSRNIYGTTKGQLRQAILWQDLDRVLAEMGPQ
                     KLRVLDAGGGEGQTAIKMAERGHQVILCDLSAQMIDRAKQAAEAKGVSDNMQFIHCAA
                     QDVASHLETPVDLILFHAVLEWVADPRSVLQTLWSVLRPGGVLSLMFYNAHGLLMHNM
                     VAGNFDYVQAGMPKKKKRTLSPDYPRDPAQVYLWLEEAGWQIMGKTGVRVFHDYLREK
                     YQQRDCYEALLELETRYCRQEPYITLGRYIHVTARKPQSKDKV"
     gene            7462..8241
                     /gene="elyC"
                     /locus_tag="OO357_002713"
     CDS             7462..8241
                     /gene="elyC"
                     /locus_tag="OO357_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="envelope biogenesis factor ElyC"
                     /protein_id="HCR5887607.1"
                     /translation="MLFTLKKVIGNMLLPLPLMLLIIGAGLALLWFSRFQKTGKIFIS
                     IGWLALLLLSLQPVADRLLRPIESTYPTWNNSQKVDYIVVLGGGYTWNPQWAPSSNLI
                     NNSLPRLNEGIRLWRENPGSKLIFTGGVAKTNTVSTAEVGARVAQSLGVPREQIITLD
                     LPKDTEEEAAAVKQAIGDAPFLLVTSASHLPRAMIFFQQEGLNPLPAPANQLAIDSPL
                     NPWERAIPSPVWLMHSDRVGYETLGRIWQWLKGPSGEPRQE"
     gene            complement(8218..9111)
                     /locus_tag="OO357_002714"
     CDS             complement(8218..9111)
                     /locus_tag="OO357_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbJ family phosphotransferase"
                     /protein_id="HCR5887608.1"
                     /translation="MEQLRAELSHLLGEKLSRIECVNEKADTALWALYDSQGNPMPLM
                     ARSFSTPGKARQLAWKTTMLARSGTVRMPTIYGVMTHEEHPGPDVLLLERMRGVSVEA
                     PARTPERWEQLKDQIVEALLAWHRQDSRGCVGAVDNTQENFWPSWYRQHVEVLWTTLN
                     QFNNTGLTMQDKRILFRTRECLPALFEGFNDNCVLIHGNFCLRSMLKDSRSDQLLAMV
                     GPGLMLWAPREYELFRLMDNSLAEDLLWSYLQRAPVAESFIWRRWLYVLWDEVAQLVN
                     TGRFSRRNFDLASKSLLPWLA"
     gene            complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="OO357_002715"
     CDS             complement(9265..10011)
                     /gene="kdsB"
                     /locus_tag="OO357_002715"
                     /EC_number="2.7.7.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-manno-octulosonate cytidylyltransferase"
                     /protein_id="HCR5887609.1"
                     /translation="MSFVVIIPARYASTRLPGKPLVDINGKPMIVHVLERARESGAER
                     IIVATDHDDVARAVEAAGGEVCMTRADHQSGTERLAEVVEKCAFSDDTVIVNVQGDEP
                     MIPATIIRQVADNLAQRQVGMATLAVPIHNAEEAFNPNAVKVVLDAEGYALYFSRATI
                     PWDRDRFAEGLETVGDNFLRHLGIYGYRAGFIRRYVNWQASPLEHIEMLEQLRVLWYG
                     EKIHVAVAQEVPGTGVDTPEDLERVRAEMR"
     gene            complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="OO357_002716"
     CDS             complement(10008..10190)
                     /gene="ycaR"
                     /locus_tag="OO357_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YcaR"
                     /protein_id="HCR5887610.1"
                     /translation="MDHRLLEIIACPVCNGKLWYNQEKQELICKLDNLAFPLRDGIPV
                     LLETEARVLTADESKS"
     gene            complement(10242..11474)
                     /locus_tag="OO357_002717"
     CDS             complement(10242..11474)
                     /locus_tag="OO357_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaQ family DNA glycosylase"
                     /protein_id="HCR5887611.1"
                     /translation="MSLPHLSLADARNLHLAAQGLLNKPRRRASLEDIPATISRMSLL
                     QIDTINIVARSPYLVLFSRLGNYPAQWLDESLARGELMEYWAHEACFMPRSDFRLIRH
                     RMLAPEKMGWKYKDAWMQEHEAEIAQLIQHIHDKGPVRSADFEHPRKGASGWWEWKPH
                     KRHLEGLFTAGKVMVIERRNFQRVYDLTHRVMPDWDDERDLVSQTEAEIIMLDNSARS
                     LGIFREQWLADYYRLKRPALAAWREARAEQQQIIAVHVEKLGNLWLHADLLPLLERAL
                     AGKLTATHSAVLSPFDPVVWDRKRAEQLFDFSYRLECYTPAPKRQYGYFVLPLLHRGQ
                     LVGRMDAKMHRQTGILEVISLWLQEGIKPTTMLQKGLRQAITDFASWQQATRVTLGRC
                     PQGLFTDCRTGWEIDPVA"
     gene            complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="OO357_002718"
     CDS             complement(11511..12497)
                     /gene="lpxK"
                     /locus_tag="OO357_002718"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HCR5887612.1"
                     /translation="MIEKIWSGESPLWRLLLPLSWLYGLVSGAIRLCYKLKLKRAWRA
                     PVPVVVVGNLTAGGNGKTPVVVWLVEQLQQRGIRVGVVSRGYGGKAESYPLLLSADTT
                     TAQAGDEPVLIYQRTDAPVAVSPVRSDAVKAILAQHPDVQIIVTDDGLQHYRLARDVE
                     IVVIDGVRRFGNGWWLPAGPMRERAGRLKSVDAVIVNGGVPRSGEIPMHLLPGQAVNL
                     RTGTRCDVAQLEHVVAMAGIGHPPRFFATLKMCGVQPEKCVPLADHQSLNHEDVSALV
                     SAGQTLVMTEKDAVKCRAFAEENWWYLPVDAQLSGDEPAKLLTQLTSLASGN"
     gene            complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="OO357_002719"
     CDS             complement(12494..14242)
                     /gene="msbA"
                     /locus_tag="OO357_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid A ABC transporter ATP-binding
                     protein/permease MsbA"
                     /protein_id="HCR5887613.1"
                     /translation="MHNDKDLSTWQTFRRLWPTIAPFKAGLIVAGVALILNAASDTFM
                     LSLLKPLLDDGFGKTDRSVLVWMPLVVIGLMILRGITSYVSSYCISWVSGKVVMTMRR
                     RLFGHMMGMPVSFFDKQSTGTLLSRITYDSEQVASSSSGALITVVREGASIIGLFIMM
                     FYYSWQLSIILIVLAPIVSIAIRVVSKRFRNISKNMQNTMGQVTTSAEQMLKGHKEVL
                     IFGGQEVETKRFDKVSNRMRLQGMKMVSASSISDPIIQLIASLALAFVLYAASFPSVM
                     DNLTAGTITVVFSSMIALMRPLKSLTNVNAQFQRGMAACQTLFTILDSEQEKDEGKRV
                     IERATGDVEFRNVTFTYPGRDVPALRNINLKIPAGKTVALVGRSGSGKSTIASLITRF
                     YDIDEGEILMDGHDLREYTLASLRNQVALVSQNVHLFNDTVANNIAYARTEQYSREQI
                     EEAARMAYAMDFINKMDNGLDTVIGENGVLLSGGQRQRIAIARALLRDSPILILDEAT
                     SALDTESERAIQAALDELQKNRTSLVIAHRLSTIEKADEIVVVEDGVIVERGTHNDLL
                     EHRGVYAQLHKMQFGQ"
     gene            complement(14279..16538)
                     /locus_tag="OO357_002720"
                     /pseudo
     CDS             complement(14279..16538)
                     /locus_tag="OO357_002720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415433.4"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC family protein"
     gene            complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="OO357_002721"
     CDS             complement(16745..17029)
                     /gene="ihfB"
                     /locus_tag="OO357_002721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006819309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HCR5887614.1"
                     /translation="MTKSELIERLATQQSHIPAKTVEDAVKEMLEHMASTLAQGERIE
                     IRGFGSFSLHYRAPRTGRNPKTGDKVELEGKYVPHFKPGKELRDRANIYG"
     gene            complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="OO357_002722"
     CDS             complement(17189..18862)
                     /gene="rpsA"
                     /locus_tag="OO357_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HCR5887615.1"
                     /translation="MTESFAQLFEESLKEIETRPGSIVRGVVVAIDKDVVLVDAGLKS
                     ESAIPAEQFKNAQGELEIQVGDEVDVALDAVEDGFGETLLSREKAKRHEAWITLEKAY
                     EDAETVTGVINGKVKGGFTVELNGIRAFLPGSLVDVRPVRDTLHLEGKELEFKVIKLD
                     QKRNNVVVSRRAVIESENSAERDQLLENLQEGMEVKGIVKNLTDYGAFVDLGGVDGLL
                     HITDMAWKRVKHPSEIVNVGDEITVKVLKFDRERTRVSLGLKQLGEDPWVAIAKRYPE
                     GTKLTGRVTNLTDYGCFVEIEEGVEGLVHVSEMDWTNKNIHPSKVVNVGDVVEVMVLD
                     IDEERRRISLGLKQCKANPWQQFAETHNKGDRVEGKIKSITDFGIFIGLDGGIDGLVH
                     LSDISWNVAGEEAVREYKKGDEIAAVVLQVDAERERISLGVKQLAEDPFNNWVALNKK
                     GAIVTGKVTAVDAKGATVELADGVEGYLRASEASRDRVEDATLVLSVGDEVEAKFTGV
                     DRKNRAISLSVRAKDEADEKDAIATVNKQEDANFSNNAMAEAFKAAKGE"
     gene            complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="OO357_002723"
     CDS             complement(18973..19656)
                     /gene="cmk"
                     /locus_tag="OO357_002723"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000125007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HCR5887616.1"
                     /translation="MTAIAPVITIDGPSGAGKGTLCKAMAEALQWHLLDSGAIYRVLA
                     LAALHHHVDVASEDALVPLASHLDVRFVSTNGNLEVILEGEDVSGEIRTQEVANAASQ
                     VAAFPRVREALLRRQRAFRELPGLIADGRDMGTVVFPDAPVKIFLDASSEERAHRRML
                     QLQEKGFSVNFERLLAEIKERDDRDRNRAVAPLVPAADALVLDSTTLSIEQLIEKALQ
                     YARQKLALA"
     gene            complement(19829..20593)
                     /gene="ycaL"
                     /locus_tag="OO357_002724"
     CDS             complement(19829..20593)
                     /gene="ycaL"
                     /locus_tag="OO357_002724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase YcaL"
                     /protein_id="HCR5887617.1"
                     /translation="MKNTKLLLAIATSAALLTGCQNTHGIDTNMAISSGLNAYKAATL
                     SDADAKAIANQGCAEMDSGNQVASKSSKYGKRLAKIAKALGNNINGTPVNYKVYMTSD
                     VNAWAMANGCVRVYSGLMDMMNDNEIEGVLGHELGHVALGHSLAEMKASYAIVAARDA
                     ISATSGVASQLSRSQLGDIAEGAINAKYSRDKESEADDFSFDLLKKRGISTQGLVGSF
                     EKLASLDGGRTQSMFDSHPPSTERAQHIRDRIASGK"
BASE COUNT         4939 a         5707 c         5018 g         4951 t
ORIGIN      
        1 ccgttatgtg ccattttttt agtgcagaaa cggagttttc ggaaaaagaa aaggcggcat
       61 tgctgccgcc ttaattttaa ctcgcctggg aaggcgcttc gtctcttcct agaagcgttt
      121 cagggagttg cggcgcaaat cctcgcaggc cgacgacgtg aacatgttcg gtattctgga
      181 agactttacg taccagttta taggtggtgc ctttttccgg actgatattt tccggcgctg
      241 cgatgatgag ctgcatttgc aaacgttcac acaattcaaa cagcgtggcg atagaacgag
      301 catccagtcg cgctgcttca tcgaggaaca gcaggcggca aggagagata tctttaccgc
      361 gcaggcggcg agattcatct tcccagcttt gtaccaccat caccagaatc gacataccgg
      421 taccaatcgc ctcaccggtc gacaatgcac cagactctgc gcgcaaccaa ccatcggaac
      481 cacggttaac ctcaacttcc atttccagat agttgcggta atccagcagt tcttcgccaa
      541 tggtctgcgg cgtgcgctgc cccatatcaa tctgcggatt aagacgctga tacaatttcg
      601 ccaacgcttc cgagaaggtc agacggttgc tgttaaacag atcctgatgc tgctcgtgct
      661 gttcagagag cacatccagc agcatggcgt gcgtttcacg cacgttcacg ttgagacgca
      721 cgctgttcac ctgaccaaac gatacgttct gcaacccctg gttgagcata cggatacggt
      781 tctgctcgcg ctgaatggtt ttgcgaatga tgttcgccac gctgcgggaa ctgatcgcca
      841 gtttctgttc acgggaggtt aattcttcgg tcagacggct aagttcaatc tccatctgtt
      901 cgatagcttc caccggatca tcggtacgaa taatatcctg acgaatacgt tcacgcagat
      961 gctgataaac cgccacgaag aactgaattt tacgctccgg acgtttcgga tcttccgaca
     1021 tgcgcagcac gtcgcgcaga tgttcgttat ccgccaccgc cagacgcagc gcacctaacg
     1081 ccttatccga catggaacgc aaatcatcag cggagagata agccagctca cgacggtgta
     1141 agcggcgctc aacgccgtta tctttcacca tgcgcatcac cgcacaccag cccgctttgg
     1201 cggtcactac ctgctcgcgc atctcaaagt aatcccgctc cagcttgcgc agtttgcggg
     1261 tcaggttgtc catctccgct tcgcagaagg taagcgcttt ttccagttga ttgcggcgtg
     1321 aacggttatt gctcagttgc gcatgcagct cgtcacggcg aatacgcgcc cgctcttctg
     1381 ccccgctatc agcacgcacg ccgatatcct gcaattcacg ttgcagatcg ttgagtagct
     1441 cttttttggt gtcgtaagaa cttttcagcg aagccagcac ctggttgtac tgactcaact
     1501 gcgctgcgtg accgcgcaac gcttcgcgag cgcgggtacg ctccgcttcc gcctgttcca
     1561 gacgttcacg cagtttttcg ttgagatcgc tgttaccgct aagcatttct gccgagtcag
     1621 aatagctaaa gtgcgcacga cgctgcacca cttccgtcag ggcaaacgct tgctgacggg
     1681 catcgcgctg catctgctga gagtacgcgt aatcttcttt taactgttcg aactgttccg
     1741 ggtcgctctg caataccgaa acgatcggtt ccagtttcgc cagttgattg ccaaactgct
     1801 gaacaaaacg cgcggcttcc tgggcttcat ccagacgttc gcggatttca tcgacgcgat
     1861 ccgccaggct gtcatcagcc aacaggttga gacgcggcag aatgcggttc agcgccgtaa
     1921 cgccttcttt cgcctgctca aactgaatac gctgctgctg gttatcattt tcatgattac
     1981 tcaacgcccg ctccagttcg actcgacggc tgttcagttg acggatttct gcttccgggt
     2041 cagactcaaa cgccaccgcc agatgactgc cgataaagcg gctgaacgcc tgatgcagac
     2101 gctgggtttt ctgtacatca aaggagagcg tggcgaagcg ttcggaaagc acttcacgct
     2161 cggcatggag gctttcaata cggctttcac gcgcagcacg accaaacagc ggcacttccg
     2221 ggaaacgtga ataacgccac tgtcgatcgg cgattttcac caccaccgct ttttccagct
     2281 catcaacgct gaacacgcta tcatcgaatg actgcggatc cccttcgatc aaatagagat
     2341 cttccgggca atcggtcaag ccttccaggt gttcggttac ctgtgacaga tctggcacca
     2401 cgatggcgtg gcgtgacggg ccatacagag ccgagaagta cggcgcatct tccaggctaa
     2461 cgtcgtcata aatttctgac agcagcacac caccaaaacg ctccgccagc gcgttcagac
     2521 gctgatcttc agagccgcca ggctggctta aacgttcgat ctcttcatcg acggcgtttt
     2581 tgcgcgcgcc tacttcatcg cgttcaacaa tcgcctcgcg ctcacgctcc agcaactgtt
     2641 gcagatattc agtgacgtcc tggctggagg agaactcttc gccgcactgt tcactcaact
     2701 ggttgagact gttttgcgct gccagccaaa ccggtgcacg ctgcatcaaa ctctgaatgc
     2761 gagactgcag ctgttcctgt tcctgccgca gtgccatccg ctcttcacgg gcgttagata
     2821 cgctatcgga aagagaggca atgcgtgctt ccagctcctg atgcagagct tccagttcat
     2881 caatatcaaa attcttgccc tgacgtttgc agaaatctgc cagcagacgc tcagcttctt
     2941 gctgctcacg cagacgctgt tccagttcgc ttaagcgcat ccgcaacggc tgaacctgct
     3001 ctgccaggtg acgctgatca accccttcgc gcaatagttc gcgagcgaca tcccacgcct
     3061 cgttacgcgc cagcgggccg ttgattgcca ccaccagctg ataagcctgc tcaaactggc
     3121 tgtgcgcggt ttgcgccatg ctcattttct gctcaagaga gagcattttt tcagtcgctt
     3181 ccagctcttt cgcctggaag gtttccagcc attcggcggc gctgtcggcg gttaagtccg
     3241 gcagatggca cagttctttg gcacgattaa gcgcagcaat cgcctggtta tactggatcg
     3301 cgcgcgtttg ctggacgtcc agcgcctgct ggtagtcagc aagctggctt ttcagctcgt
     3361 ccacttccag ttcggcagct tccgcgcgag cctcattctc ttcctggcgt tcgatggctt
     3421 ctgccaccac ttcattttgc tcttccagac ggatctgcag ctcatcgaga tccacttcgt
     3481 agcgttcgat cttctcctgc tgacgcaatg cggtttgcac caggttcagg tgatcgctgg
     3541 ccgcctgata atccgcttcc agatcacctt cggcaccgtt gtgctctgcc agctcacgcg
     3601 ccatatcgac gtgtttgtac tgctcagccg ccagttgctg acgcgaagta tgtagctcgc
     3661 gacgaaactc cagggctttg tcgagatgga cacggcgctc gttggcgtga cgcatgtagt
     3721 ccgccgccac gtagttggta gcttcgctga tcagatgctt aaacaggtca cggtccgact
     3781 gggtgacacg aatcgcttcc agcgtcatgc ggttttcacg cagcgccgct tccatgtcct
     3841 ggaacgcttt acgcacgccg ctgttttccg gcaacaggta gtcgcgcaga gaacgggtaa
     3901 tggcactgga gatcccgcca tacagcgaag cttcgatcag acgatagaat ttgctacggt
     3961 cagatgcgga gcgcagacga cgcgcgatga tgcccagatc gaacatcagc gagtggtaat
     4021 cggtaatgga gttgaactgt ttaaactgca cgccttccat cgcctcgagc ttgtctttca
     4081 gctcgttaag cggcagcacg cgcgcctggc gttcgttcag ggtttcggtc accagctgtg
     4141 tcggctgcac cgacatcggc agtccctgaa tggcaaatgg tttgatatcc actttacgat
     4201 cgcgtccggc aacctgttgc agacgcacac cgaccaccac gcgctggtgg cgcgagttaa
     4261 tggtgtcgag catcgaataa cagacacccg ctttcagctt accgtgcaga cctttatcgc
     4321 gcgaaccgct ggtggccccg gcttccgtag tgttacggaa atgcagcagg gtcaggtcgg
     4381 ggatcagcgc cgtaacgaac gccgccatcg tggtggattt acccgccccg ttaccgccgg
     4441 aaagcgtcgt gaccagctcg tcaaggtcaa aagttcgggc aaaaaagccg ttccagttaa
     4501 tcagcgtcag tgagcgaaat ttaccgcgtt caatcattat tcttcctctc cgctatctgg
     4561 ctgactctct tcggtttcat cgttgagttg cagatgattt tcaatcggca ttgcttcgcc
     4621 atcacgaatc aggcgacgct gtgcttcacg gggatcgtcg ccagcacgca catcggctcc
     4681 gaagcggaac accgattcgg taatgcggaa cttgctgctg tcgtggccca taaaccacac
     4741 catgcctaaa cgacgcagac ggttgagcga agaacgtact ttctcctgca acttctgacg
     4801 gtcaacgtct gaaccggttg aacggttgtt caccagtttc agcagtttcg cttcatcggc
     4861 cagggtgagc agttcgtcgt acagttcctg ctgggtgaaa atcccctcat tcgccagccg
     4921 ttccgggctg agatagagat aacagaggat tttcccgacc atcatatcca gttccgacaa
     4981 gacggaacgc gggataagag tggtggaacg tgggcgtaaa tagaagaacc cttccggtgc
     5041 gcgaataagc tcaacgttat aacgcgcgta aaactcttcc agatattcct gaaaatccat
     5101 caggaatgca tgattatcca gttcgtcgag gccaatatgg cggcctgaac gtaaggcgct
     5161 gtccagcgcc ggaaataacg gattcgccag cgcctgcgcc agcttaaccg gcatcacttg
     5221 ttcaatattt gtcgatgaca tgcgcctgta ccttggctcc gtaatcatta atcggctgcc
     5281 atttcgctgg cagtccggtg aaatctgctt gcgctacgcc aagacgtacc gcctgatcaa
     5341 taacaatacg cgcaacgtca aagtgacgtg cacgcggata ctgtgacaga tattcgcgta
     5401 ccaccagacc aagatccagc ggcacttgtc tggttttgta cacggcaagt tgttcttcga
     5461 tgatcgccgc cagctgttcg cggatctcgt taaactcttc gtattccaga tcctcaggaa
     5521 gttccccagt cacttcttca tcgcgcagtg ccatctcttc gtcacgcata tccagcagac
     5581 gatcggcatt ggcataagtt agcgcccacg gctcatcaaa ataggtttgt accgactgac
     5641 gtaaccgctg agcaaagacg cggtttttat ccatatcgat cgcggtacga ataaatttgt
     5701 gtacgtggcg gtcgtagcca atccacaagt cgatggattg ctggccccaa ctgataatac
     5761 gatcgagttt gctctgcaga tcgaacacca gacgatcgac gaaatgcaga tcgtcatggg
     5821 tcatcgtcgc atcctgaatg cgcaacagat tagcctgcaa tttgtcgcct gccgcttcca
     5881 gcgtatcctg caattcacgc agcgttccgg aagtttccga aagcaacaat tcacagctgg
     5941 aaatcgccgc ccgccagtct ttgttcagca actgggcgat atcgtccttc acctgctgct
     6001 gctgttcgtc catcagacgt tgcgtcaggt cgatactgtc gaaaatttct gctaccgaat
     6061 atttcagtgg cgcatagaca ttacggtgcc agtgaaattc atcaccgccc tcttcggcgg
     6121 catctgctgc gcgtttgagc tcacccgcca caatcgacaa ctgcatagaa agacgcagcg
     6181 tagaaaactc gcgctgacgg atgtagtagt cagtaatgcc gatgccgagc ggcgtcagac
     6241 ggtaaattgc gttcccttcc gcctgctcgc tggtaaagcg gttcagcaga cgttgacgca
     6301 ccatgtcgtt gatcgcgtta ttggcgcgca cgccgatggt ttcgctggtt tgctcaaacg
     6361 catcactcac atggcggaat gcatccacca gctcgccttc actcatctca ccatccagac
     6421 gctcgccgtt cagcgtggca accgccagca gaaaagagag tcggtctacc ggcagcgaga
     6481 tggagaagtc attttttctg gcccaggcaa ccagttcggg gactgtctgg gaaaattcac
     6541 tcatacttta tccttgctct gcggtttgcg cgcggtgaca tgaatataac gccctagggt
     6601 aatatacggt tcctgacggc aataacgcgt ttctaattca agtaatgctt catagcagtc
     6661 gcgctgctgg tacttctcgc gcagataatc atgaaacacg cgaacgcctg tcttacccat
     6721 aatttgccaa ccagcttctt ccagccacag ataaacctgc gccgggtcgc gtggataatc
     6781 tggcgaaagc gtccgttttt tctttttcgg cattcccgcc tgcacgtaat caaaattccc
     6841 ggcgaccatg ttatgcatca acaaaccatg cgcattgtag aacattaacg acaacactcc
     6901 gcctggacgc aacactgacc acagggtctg caatacactg cggggatcgg ccacccactc
     6961 gagcaccgca tggaacaata tcagatcaac gggcgtttcc aaatgcgaag caacatcctg
     7021 agcggcgcaa tgtataaatt gcatgttgtc gctcacacct tttgcttctg ccgcctgttt
     7081 tgcgcggtcg atcatctgcg cagaaagatc gcataaaata acctgatgcc cgcgctcggc
     7141 cattttgatt gcggtctgcc cttctccgcc gccagcatcc agcacacgca gtttttgcgg
     7201 gcccatttcc gccagcacgc gatcgagatc ctgccacaga atagcctgtc gaagctgccc
     7261 tttggtggtg ccgtaaatgt tacgggaaaa cttttccgca atatcatcaa aattgcgatc
     7321 ctgcattgct ttctccaccg gttggctaag acgttttctg ataaagacgc tattttgtca
     7381 cagccacggc gagaatgaac ccgtctggtg taatatcgct agcgatccct tgctatatgg
     7441 ttaaaaaagg aaccagaaag gatgcttttt acactgaaaa aagtgattgg caatatgttg
     7501 ctgccacttc cgctgatgtt gttaattatt ggcgctggcc tggcgctgct gtggtttagc
     7561 cgttttcaaa aaaccgggaa gatttttatt agcattgggt ggctggcact cttgttattg
     7621 agtttgcaac cggtagccga ccgcctgctg cgtcccatcg aaagcaccta tccgacatgg
     7681 aataattcgc aaaaagtgga ctatatcgtg gtgctcggag gtggttatac ctggaacccg
     7741 cagtgggcac cgagctctaa tttaatcaat aacagtctgc cacgcctgaa tgagggtatc
     7801 cgtttatggc gcgaaaatcc gggttcaaaa ctgatcttca cgggaggcgt agcaaaaacc
     7861 aatacggtga gtacagcgga agtaggtgcc agagttgcgc aatcgctggg cgtaccgcgc
     7921 gagcaaatta tcaccctgga tttgccaaaa gataccgaag aagaagctgc ggcagtgaaa
     7981 caggcgattg gtgacgcccc tttcctgctg gtgacctccg catcacatct gccgcgggcg
     8041 atgatctttt ttcagcagga agggttaaat ccgctaccag ccccggcgaa tcagttggcc
     8101 atcgactcgc cgcttaatcc gtgggaacgg gcaatccctt ctccggtctg gctgatgcat
     8161 agcgatcgtg ttggctatga aacgcttggc cgcatctggc agtggctgaa aggtccgtca
     8221 ggcgagccac ggcaagagtg attttgatgc cagatcgaag ttgcgccgac taaatcgtcc
     8281 ggtattaacc agttgcgcaa cttcatccca taacacatac aaccaacgcc gccagatgaa
     8341 cgactccgcc actggcgcgc gttgcaggta actccagagc aaatcttccg ccagagaatt
     8401 atccattagt cggaacagtt cgtattctcg cggtgcccaa agcattagtc ccggcccgac
     8461 catcgccagt aactgatcgc tgcgcgaatc tttcagcatg ctgcgtaaac agaagttacc
     8521 gtgaatcaac acacaattgt cgttaaagcc ttcaaataat gccgggagac attcgcgagt
     8581 gcgaaacagg atccgcttat cctgcatcgt cagaccggtg ttattgaact gattgagcgt
     8641 ggtccatagc acttcaacat gttgccggta ccatgagggc cagaaatttt cctgagtatt
     8701 gtcgaccgcg ccgacgcaac cgcgactgtc ctgacggtgc caggccagta aggcttcaac
     8761 gatttggtct ttgagctgtt cccagcgttc tggtgttcgg gctggtgcct ccaccgaaac
     8821 accacgcatc cgctccagta gcaggacatc agggccgggg tgttcttcat gcgtcatcac
     8881 accataaata gtcggcatac ggacagtccc acttcttgcc agcatggtgg ttttccatgc
     8941 cagttgtcgg gcttttccgg gcgtactaaa gcttcttgcc attaacggca ttgggtttcc
     9001 ctggctgtca tacaaagccc acaacgccgt atccgctttt tcattgacgc actcaatacg
     9061 actgagtttt tcgcccagta aatggcttaa ttcggcacgc agctgttcca ttcagattac
     9121 cctcataaaa gctactgctt tcataatgag cgccaatata aaagatgtca cctgaatgag
     9181 atcaattgtt gaaaagaaaa attgaggaaa atcgtaacgg tacgacactc ctcccaaaat
     9241 tggctgaagt gtcgtgaagt gaaattagcg catttcagcg cgaacgcgct caagatcttc
     9301 aggggtatcc acacctgtgc caggaacttc ctgagcaaca gcaacatgga ttttttcgcc
     9361 gtaccacaga acacgaagct gctctaacat ttcgatgtgt tctaacggac ttgcctgcca
     9421 gttgacgtaa cgacggataa agcctgcacg gtagccataa ataccaagat gacgcaggaa
     9481 gttatcgcca acggtttcaa ggccttctgc aaaacgatca cgatcccaag gaatggtggc
     9541 acgtgagaag tacagtgcat acccttcagc gtcgagaacc actttcaccg cattcgggtt
     9601 aaacgcttct tccgcattgt ggattggcac cgccagagtc gccataccca cctgacgctg
     9661 agcgaggtta tcagcaacct gacgaatgat tgtcgcaggg atcatcggtt catcaccctg
     9721 cacattaacg atcaccgtgt cgtcgctgaa tgcgcatttt tcgacaactt ccgccagacg
     9781 ttctgttcct gactgatgat cggcgcgcgt catacatact tcaccgccag cggcttcaac
     9841 ggcgcgggca acatcatcat gatcggttgc cacgatgatg cgctcggcac ctgattcacg
     9901 cgcgcgttca agaacatgaa caatcatggg tttgccgtta atatcaacca atggtttacc
     9961 gggcagacgc gtggacgcgt agcgcgcggg aataatgacc acaaaactca tgatttactc
    10021 tcatcagcag tcagcacgcg ggcttccgtt tccagcaaca ctggaatgcc atcgcggagc
    10081 gggaaagcca ggttatccag tttgcaaata agctcttgtt tttcctggtt ataccaaagt
    10141 tttccgttgc aaaccgggca ggcaatgatt tcaagcaaac gatgatccat agttcctcca
    10201 gatggatcgg gttatgaatg cataaatctt atcataatca tttatgcgac ggggtctatt
    10261 tcccagccag tgcggcaatc agtaaagaga ccttgcgggc agcgtcctaa tgtcacccgc
    10321 gttgcctgct gccagctcgc gaaatcagta atcgcctgac gtaacccttt ttgcagcatt
    10381 gtcgttggtt taatgccttc ctgtaaccac agagagataa cttcaaggat gcctgtctgg
    10441 cgatgcattt tggcatccat tcgcccaact aattgcccac gatgtaataa tggcagaaca
    10501 aaatagccat actggcgttt cggcgctggg gtatagcact ccagccggta gctaaaatca
    10561 aaaagctgct ctgcgcgttt gcgatcccag acaacaggat caaaaggcga aagtaccgcg
    10621 ctgtgcgttg cagtgagctt tccggccagc gcacgctcga gtagcggcag caaatcagca
    10681 tgcagccaaa gattgcccaa tttttcaaca tgcacagcaa tgatttgctg ctgttcagcc
    10741 ctcgcttcgc gccacgccgc cagtgccgga cgtttcagcc gatagtaatc tgccagccac
    10801 tgttcgcgga atattcccag gctacgcgca ctgttatcca gcatgatgat ttctgcttct
    10861 gtttgcgaaa cgagatcgcg ctcatcatcc cagtcaggca tgacacggtg ggttaaatca
    10921 taaacgcgct ggaagttgcg ccgttcaatc accatcacct ttccggcagt aaataaacct
    10981 tccagatgcc gtttatgcgg cttccattcc caccagccgc ttgcaccttt acgaggatgc
    11041 tcaaaatcgg ctgaacgtac cggcccttta tcatgaatat gctgaattaa ctgtgcaatt
    11101 tccgcctcat gttcctgcat ccaggcgtct ttgtatttcc agcccatttt ttcaggtgcc
    11161 agcatgcggt ggcgaataag acgaaagtcg ctacgcggca taaagcaggc ttcatgcgcc
    11221 cagtattcca ttaattcgcc acgcgccaga gactcatcca gccactgggc aggataattt
    11281 cccagacgac tgaaaagcac cagatatgga ctacgggcaa caatattgat ggtatcgatt
    11341 tgcagcaagg acatgcggga gatcgttgcc ggaatatcct ccaacgacgc tcgacggcgg
    11401 ggtttgttta acaggccttg tgcggcaagg tgaagattac gcgcatcagc aagggagagg
    11461 tgcggcagcg acattcatga ctccatcaat cgaacgctgc cgcggcgtaa ctagttgcca
    11521 gaagccagcg aggttagttg cgtaagcagt ttcgctggtt catcacctga aagctgtgcg
    11581 tctacaggca aataccacca attttcttct gcaaaggccc ggcatttcac cgcatctttt
    11641 tcagtcatta ccagcgtttg cccggcgctt accaacgcac tgacatcctc atggttcaaa
    11701 gactgatgat cggccagcgg tacacatttt tccggttgta cgccacacat cttcagcgtg
    11761 gcaaaaaagc gcggcggatg cccaatcccc gccatcgcca ctacatgttc aagctgagca
    11821 acgtcacaac gcgtaccggt acgtaaattc accgcctgac ccggcagcag atgcatgggg
    11881 atttcaccgc tgcgagggac accgccgttg acgattaccg catcaaccga ctttaagcgc
    11941 cccgctcgct cacgcattgg ccccgccggc aaccaccagc cattgccaaa gcgacgcaca
    12001 ccatcaataa cgacaatttc cacatcacgc gccagacggt aatgctgtaa accgtcgtcg
    12061 gttacgatga tctgcacatc agggtgttgc gccagaatgg cttttaccgc atcagaacga
    12121 acgggagaaa ccgcaacagg cgcatcagtg cgttgataaa tcaacacagg ttcatcaccc
    12181 gcctgtgctg tggtggtatc tgccgacaat aacagcggat aagattcagc cttaccacca
    12241 tatccccgcg atacgacccc cacgcgaata ccgcgctgtt gcaactgttc caccagccag
    12301 acaacgaccg gggtttttcc gttgccgcct gcggtgagat taccaaccac gacaaccggt
    12361 acgggggcac gccaggcgcg cttcagtttt agtttatagc aaagacggat cgcgccactc
    12421 accaggccat acaaccagga gagtggcagc aatagccgcc acaaagggga ttcaccagac
    12481 cagatttttt cgatcattgg ccaaactgca ttttgtgaag ttgcgcgtaa acgccgcggt
    12541 gctcaagcaa atcgttatgc gtaccgcgtt ccacaatgac accatcctcg acgaccacga
    12601 tttcgtctgc cttttcaatg gtagacaagc ggtgggcaat caccagagag gtacggtttt
    12661 tctgcaactc atccagtgcc gcctgaatcg cacgttcgga ttcggtatcc agagccgagg
    12721 tagcttcatc cagaatcaga atcgggctat cacgcagcaa ggctcgagcg atagcaatac
    12781 gctgacgctg accgccagag agcagcacgc cgttttcacc aatcactgta tcgagaccgt
    12841 tatccatctt attgatgaag tccatggcgt aggccatccg cgccgcttct tcaatttgct
    12901 cacggctgta ctgttcagtc cgtgcgtaag caatgttgtt agcaaccgta tcgttaaaca
    12961 gatggacatt ctgcgacacc agagcaacct ggttacgtaa cgacgccagg gtatactcgc
    13021 gcagatcgtg accatccatc aggatttcgc cttcatcaat atcgtaaaaa cgcgtgatca
    13081 ggctggcgat ggttgattta cctgaaccag agcgtccaac cagagcaacc gtcttccctg
    13141 ccggaatttt caggttgatg ttacgcaatg cgggtacgtc acgtcccgga taagtaaagg
    13201 tgacattgcg gaattccacg tcgccagtcg cacgctcgat cacgcgctta ccttcgtctt
    13261 tctcctgctc actgtccaga atggtaaaca gcgtctgaca agccgccata ccgcgctgga
    13321 actgggcgtt aacgttggtc agcgatttca gcggacgcat cagtgcaatc attgaagaga
    13381 aaacaacggt aatcgtaccg gcagtcaggt tatccataac acttgggaag ctcgccgcat
    13441 acagaacaaa cgccagcgcc aaagaggcga tcagctgaat gatcggatca gagatggaag
    13501 aggctgaaac cattttcatc ccctgaagac gcattcggtt actgacttta tcgaagcgtt
    13561 tcgtttccac ttcctgacca ccgaaaatca atacttcttt gtggcccttc agcatttgtt
    13621 ctgcactggt ggtcacttgc cccatggtgt tctgcatgtt tttactgatg ttgcgaaaac
    13681 gcttcgatac tacgcgaatc gcaatcgaaa caatcggtgc cagcacaatc aaaatgatcg
    13741 acagttgcca actgtaatag aacatcatga tgaacaggcc gatgatcgac gcaccttcac
    13801 gcacaacagt aatcagtgcg ccggaagaag aagaagcaac ctgttcggaa tcgtaggtga
    13861 tacgtgacaa cagcgtaccc gttgactgtt tgtcaaagaa tgaaactggc attcccatca
    13921 tgtgaccaaa caggcggcga cgcatggtca ttaccacctt tcctgatacc caggagatac
    13981 agtagctgga gacatagctg gtgataccac gtaaaatcat cagtccgatc accaccagcg
    14041 gcatccacac cagcacggag cgatctgttt taccaaagcc atcatcaaga agtggcttaa
    14101 ggagcgataa catgaaggta tcgctggctg cgttgaggat taacgctacg cccgccacga
    14161 tcagacccgc tttgaaaggc gcaatggttg gccacagtcg gcggaatgtc tgccacgtag
    14221 agagatcttt gtcgttatgc attcaaaaaa ccagcatttg ttgaaatagc cgcatattct
    14281 acccgttatc cactggcacg ccaaaccact gatgatacca acgaggtaaa atttgctctc
    14341 tgaggctgct aatccgccag ccttgtgctg aaaaattgac cgtcacttgc ccctgatgtg
    14401 gagtatcaag ccattgatat cctttctgtt gatagcgatg cttaactttg ttagagggca
    14461 atcgccatgc gttatagcgc gatgccgatg cgagtgccac ttttccattc actcgctgaa
    14521 ttaatggcaa cgatgatgag gtattactgc catggtgagg tacctgaagc aatgttgcct
    14581 gcacttgctg ccagtaacgg cttagcatct tttgttcagc tggggcttca atatcaccgg
    14641 ttagaagaat gctattcgtc ccgtcatcaa ccttaaccac acaggaatgg ttatttccct
    14701 tatcgttgct accttgtaaa ggccagtgcg cgctgaaacg caatccttgc cactgccacg
    14761 cttcgccacg cacacagggc tgatgatgtt cccagtttaa cggacttctg atccataaca
    14821 tcggccatat atgcaatatt gaatccagcc ctccccggtg atccagatgt tcatggctca
    14881 gaataacgcc ttccggttca agattatgcc agtggagcca ggggataatc agttgttgcc
    14941 cactatcccc ttcaggccag tcctgtgtca tagagaatcg ctttgccgtt tctggctatc
    15001 accattgcca gcccttgccc gacatcaagc atgtacagct gccactcgtc aggtcgaggt
    15061 ttttgccaca gcggccagca catcagcaag cctccagcca cacacattgc tggcaacgtt
    15121 cgccaggcgt ttaatcgcca taccaccagt aagaaccatg gggaaaatga tagccattgc
    15181 caacattcaa caatgttgat ccacccttcc ggcaaactct ttaaccccca gaaaagtaaa
    15241 gccaaagacc ggtcggcaag aaaccataac ccctgttcca ggattaacgg cccgctaaaa
    15301 tgcacaacca tcgcggcgag gatcaacgga accgtgataa atgtcaccaa gggaattgca
    15361 aatagatttg caataaacga ggtcagacta atgccatgaa atatgacgat ttgcatgggc
    15421 ataagcagga gtgtgattcc cagttgcaga tggatgaggg aaacaactgc acgcaatacc
    15481 ggcggcagtt gccactcagg acagggaaac cactgatacc aaaaaatcag tgccgcgacc
    15541 gcagcggcag agagccataa actttgcgag agaatggcaa caggatccat cagcaaaatt
    15601 gccgccagac aacatatcca tacatcccag ccactccact gtcgcccact taacttaagc
    15661 attccccacg tagcaagcgc caccacggta cgcaatgcag gaggttgcat tcccgtcaac
    15721 caggcataaa aagcagcaca gcagattccg ccaattaatg gtatttgcca gtggatccag
    15781 cgcccaggca gaaaaatttg tccactgcga atgagtccgg cagccagcaa cgccgcaaaa
    15841 gcgatgtgca atcccgatat cgccattaaa tgcgccgttc cagtatcgcg catgatattt
    15901 ttgatttctt tggggacgga taaccgttcc cccataccta aaccaagaat aaccgcattc
    15961 cacggatagg gttgcagcgt tgtttgtagt gacgccagat actgtgcacg caggctacaa
    16021 ttcggttcaa tgacacttgc ctgcagaaaa cggccggtga gcggctgatg ctgggcaatg
    16081 gcataacgct ggctatcaaa gccgccatca ttaagttggc catgaactgc acgaactttg
    16141 agtttcattg accattgttg tccagcacaa accgcttgcg gaagttattc gccatacagc
    16201 acaaggcctg gcgcagggaa tatacgtcga ccttgtagat aagtaatttg accgtaatga
    16261 gtggtcatgc cgtcagttgc agtgatctca acaattgcat cctgcgtcgc gccagttaag
    16321 gtttctcctg cccacaaaat ttgctttgct gataatatgc cccaaacgaa aaacagcaac
    16381 gtcagcgcga tataacggac ggttttaaca gggataaatg ccagtacgca ggcgaagaga
    16441 gtcagaaacg caagggttaa tgtcctaggc aattggggca aaatcagcaa cggaaaaatt
    16501 ccgcaaatta tgcatacgcc gactgtcgtt attttcatgc acccctccct gtttatggaa
    16561 agtgtgcaac tttgttgagc ggttgtctgg tgggaatttt gcgttccccg aggcacattc
    16621 agaagaattt aacgaaaggg tgaaaactgc tcaaaactct gcgaggcagc ttccagttcc
    16681 tgattagaga attgaaaaaa gcacccgaca ggtgcttttc tcgcgttcaa gtttgagtaa
    16741 aaacttaacc gtaaatattg gcgcgatcgc gcagttcttt accaggttta aagtgaggaa
    16801 cgtattttcc ttccagttct actttatcgc cagtcttcgg attacgtccg gtacgtggtg
    16861 cgcggtagtg caaagagaaa ctgccgaaac cgcggatttc aatacgctcg ccctgcgcaa
    16921 gagtcgaggc catatgctcc agcatctctt ttactgcatc ttcaaccgtc ttggcgggaa
    16981 tgtgcgattg ctgggtggca agtctttcta tcaattctga cttggtcatg attcctccgg
    17041 ttccttaaag gcaaattaag cggctgctgt tgctgcattg attaagggcg gccgtagccg
    17101 cccttagtgc ttgattacag gacgaaacct gcaatctgtc aagtaaactc aacaaacttc
    17161 ggaataaaaa tcccgaagag tcagagaatt actcgccttt agctgctttg aaagcttcag
    17221 ccattgcgtt gttggagaag tttgcatctt cctgtttgtt aacagttgcg attgcatctt
    17281 tctcgtcagc ttcgtctttc gcacgaacag acaggctgat tgcgcggttt ttacgatcaa
    17341 cgccggtgaa tttagcttca acttcgtcgc caacgctcag aaccagggta gcgtcttcaa
    17401 cgcggtcacg ggatgcttca gaagcacgca ggtaaccttc aacgccgtca gccagttcta
    17461 cggttgcgcc tttagcgtca actgcagtta ctttaccggt tacgatagcg cctttcttgt
    17521 tcagagcaac ccagttgttg aacggatctt ctgcgagctg tttaacgccc agggagatac
    17581 gttcacgttc tgcgtcaacc tgcagaacaa ctgcagcgat ttcgtcgcct tttttgtatt
    17641 cacgaactgc ttcttcgcct gcaacgttcc aggagatgtc agacaggtga accaggccgt
    17701 cgatgccgcc gtccaggccg atgaagatac cgaagtcagt gatagacttg attttacctt
    17761 caacacggtc gcccttgttg tgggtttccg cgaactgctg ccacgggtta gctttgcact
    17821 gtttcagacc cagggagata cgacgacgtt cttcgtcgat atccagaacc ataacttcca
    17881 ctacatcgcc aacgttaaca actttggacg ggtggatgtt tttgttggtc caatccattt
    17941 cggaaacgtg taccagacct tcaacgcctt cttcgatttc aacgaagcag ccgtagtcag
    18001 tcaggttggt cacgcgacca gtcagtttgg taccttccgg ataacgttta gcgatagcta
    18061 cccacggatc ttcgcccagc tgtttcaggc ccagggatac acgggtacgt tcgcggtcga
    18121 acttcagcac tttaacagtg atttcgtcgc ccacgttgac gatttcgctc ggatgcttaa
    18181 cgcgtttcca ggccatgtca gtgatgtgca gcaggccgtc aacgccgccc agatcaacga
    18241 atgcaccgta gtcagtgagg ttcttaacga tacctttaac ttccatgcct tcctgcaggt
    18301 tttccagcag ctgatcgcgc tctgcgctgt tttcggattc gataacggca cgacgagaaa
    18361 caacaacgtt gttgcgcttc tgatccagct tgattacttt aaattcaagc tctttgcctt
    18421 ccaggtgcag agtgtcacgc accggacgaa cgtctaccag agaacctggc aggaacgcac
    18481 gaataccgtt cagctcaaca gtgaagccgc ccttaacttt gccgttgata acaccggtaa
    18541 cagtttcagc atcttcgtaa gctttttcca gcgtgatcca ggcttcgtga cgtttagctt
    18601 tctcacggga cagcagagtt tcaccgaagc cgtcttctac tgcgtccaga gcaacgtcaa
    18661 cttcgtcacc tacctggatt tccagctcgc cctgggcgtt tttgaactgc tcagccggga
    18721 tggcggactc agatttcaga ccagcgtcaa ccagtactac gtctttgtcg atagcaacaa
    18781 caacgccacg aacgatagaa cccgggcggg tttcgatttc ttttaaggac tcttcaaaga
    18841 gttgagcaaa agattcagtc atgtttaatc ttcaggttta tatttaacgt ccacctggct
    18901 ccatgccgga tggggttgtt taacataccc gctgtcattc cattgcaacg ggggtactgc
    18961 aaattcggtc gcttatgcga gagccaattt ctggcgcgcg tattgtagcg ctttttcaat
    19021 caattgctca atgcttaagg tggtggaatc caacactaaa gcatcggctg ccggaaccag
    19081 tggcgctacc gctcggttac gatcgcggtc gtcgcgttct ttgatctcgg ccaaaaggcg
    19141 ctcgaagtta acactaaagc ccttctcctg caactgtagc atgcggcgat gcgcacgttc
    19201 ttccgaggag gcgtcaagga aaattttcac tggtgcatca gggaatacca ccgttcccat
    19261 gtcgcggcca tcggcaatca gacctggtaa ttcgcgaaac gcgcgttggc gacgcaataa
    19321 tgcttcacga acgcgtggga atgccgcgac ttgtgaagcc gcattcgcca cttcctgagt
    19381 acgaatttcg ccgctgacat cttccccttc gaggatcact tccagattgc cattggtcga
    19441 cacaaaacgt acatccagat gggatgccag cggtaccagc gcatcttccg acgcaacatc
    19501 aacatggtga tgtaatgccg ccaatgccag tacgcgataa attgcacccg agtccagcag
    19561 atgccattgc aacgcttccg ccatagcctt acacaaggtg cctttccctg caccgcttgg
    19621 gccatcaatg gtaataaccg gggcaattgc cgtcatcttt atctccttaa aacaggcgta
    19681 ccgttaacat aaccgcgcgc attatacgcg ccgacgccac aaatcgttaa cgttgtgtgc
    19741 aaaaaccagt tttgtctgta ttcagatggg gatttattgc gcaattatag cgggctggca
    19801 gaagaccagc ccgaaagatg acaatgattt acttaccaga ggcgatacga tcacggatgt
    19861 gttgcgcacg ctctgttgat ggtgggtgag agtcaaacat ggactgggtg cgaccgccat
    19921 ccaggctagc cagtttttca aagctgccaa ccagcccctg ggtgctgatg ccacgtttct
    19981 tcaacagatc aaaagagaaa tcatctgctt cggactcttt atcacgggag tatttagcat
    20041 taatagcgcc ttctgcgata tcacccaatt gtgagcggga aagctgggaa gccacaccgc
    20101 tggtagctga aatggcatcg cgtgcggcaa cgatcgcata agaagctttc atttcagcca
    20161 gcgagtgacc caacgcgacg tggcccagtt catggcccag aacaccttca atttcgttgt
    20221 cattcatcat gtccatcagg ccactgtaga cacgaacaca gccgttcgcc atcgcccatg
    20281 cgttgacgtc gctggtcata taaaccttat agttgaccgg cgtgccatta atattgttac
    20341 ccaatgcttt ggcgattttt gccagacgtt taccgtactt gctggattta cttgcgactt
    20401 gattgccgct gtccatttcg gcacagccct gattggcaat cgcttttgca tcggcatcgc
    20461 ttaatgttgc tgctttatag gcatttaaac cggagctgat agccatattg gtatcaatac
    20521 cgtgggtatt ttgacaccct gtcagtaatg ctgcagaggt cgcaatcgcc agcagtaatt
    20581 tagtattctt catgtgaatc atccgttgat taatc
//
LOCUS       DAJTRC010000070        20614 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.070, whole genome shotgun sequence.
ACCESSION   DAJTRC010000070 DAJTRC010000000
VERSION     DAJTRC010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20614)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20614)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20614
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.070"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..145)
                     /locus_tag="OO357_002725"
     CDS             complement(<1..145)
                     /locus_tag="OO357_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF986 family protein"
                     /protein_id="HCR5887618.1"
                     /translation="MTITDLVLILFIAALLAFAIYDQFIMPRRNGPTLLAIPLLRRGR
                     IDSV"
     gene            complement(200..1051)
                     /gene="manZ"
                     /locus_tag="OO357_002726"
     CDS             complement(200..1051)
                     /gene="manZ"
                     /locus_tag="OO357_002726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020804907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IID"
                     /protein_id="HCR5887619.1"
                     /translation="MVDTTQTTTEKKLTQSDIRGVFLRSNLFQGSWNFERMQALGFCF
                     SMVPAIRRLYPENNEARKQAIRRHLEFFNTQPFVAAPILGVTLALEEQRANGAEIDDG
                     AINGIKVGLMGPLAGVGDPIFWGTVRPVFAALGAGIAMSGSLLGPLLFFILFNLVRLA
                     TRYYGVAYGYSKGIDIVKDMGGGFLQKLTEGASILGLFVMGALVNKWTHVNIPLVVST
                     ITGQDGQTRVTTVQTILDQLMPGLVPLLLTFACMWLLRKKVNPLWIIVGFFVIGIAGY
                     ACGLLGL"
     gene            complement(1064..1864)
                     /gene="manY"
                     /locus_tag="OO357_002727"
     CDS             complement(1064..1864)
                     /gene="manY"
                     /locus_tag="OO357_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002910918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIC"
                     /protein_id="HCR5887620.1"
                     /translation="MEITTLQIVLVFIVACIAGMGSILDEFQFHRPLIACTLVGIVLG
                     DMKTGIIIGGTLEMIALGWMNIGAAVAPDAALASIISTILVIAGHQSIGAGIALAIPL
                     AAAGQVLTIIVRTITVAFQHAADKAADNGNLTAISWIHVSSLFLQAMRVAIPAVIVAL
                     SVGTSEVQNMLNAIPEVVTNGLNIAGGMIVVVGYAMVINMMRAGYLMPFFYLGFVTAA
                     FTNFNLVALGVIGTVMAVLYIQLSPKYNRVAGAPAQAAGNNDLDNELD"
     gene            complement(1927..2898)
                     /gene="manX"
                     /locus_tag="OO357_002728"
     CDS             complement(1927..2898)
                     /gene="manX"
                     /locus_tag="OO357_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HCR5887621.1"
                     /translation="MTIAIVIGTHGWAAEQLLKTAEMLLGEQENVGWIDFVPGENAET
                     LIEKYNAQLAKLDTTKGVLFLVDTWGGSPFNAASRIVVDKEHYEVIAGVNIPMLVETL
                     MARDDDPSFDELVALAVETGREGVKALKAKPVEKAAPAPAAAAPKAAPTPAKPMGPND
                     YMVIGLARIDDRLIHGQVATRWTKETNVSRIIVVSDEVAADTVRKTLLTQVAPPGVTA
                     HVVDVAKMIRVYNNPKYAGERVMLLFTNPTDVERLVEGGVKITSVNVGGMAFRQGKTQ
                     VNNAVSVDEKDIEAFKKLNARGIELEVRKVSTDPKLKMMDLISKIDK"
     gene            3361..4911
                     /gene="yoaE"
                     /locus_tag="OO357_002729"
     CDS             3361..4911
                     /gene="yoaE"
                     /locus_tag="OO357_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CNNM family cation transport protein YoaE"
                     /protein_id="HCR5887622.1"
                     /translation="MEFLMDPSIWAGLLTLVVLEIVLGIDNLVFIAILADKLPPKQRD
                     KARLLGLSLALIMRLGLLSLISWMVTLTKPLFTVMDFSFSGRDLIMLFGGIFLLFKAT
                     TELHERLENRDHDSGHGKGYASFWVVVTQIVILDAVFSLDAVITAVGMVNHLPVMMAA
                     VVIAMAVMLLASKPLTRFVNQHPTVVVLCLSFLLMIGLSLVAEGFGFHIPKGYLYAAI
                     GFSIIIEVFNQIARRNFIRHQSTLPLRARTADAILRLMGGKRQANVQHDADNPMPIPE
                     GAFAEEERYMINGVLTLASRSLRGIMTPRGEISWVDANLGVDEIREQLLSSPHSLFPV
                     CRGELDEIIGIVRAKELLVALEEGVDVAAIASASPAIIVPETLDPINLLGVLRRARGS
                     FVIVTNEFGVVQGLVTPLDVLEAIAGEFPDADETPEIITDGNGWLVKGGTDLHALQQA
                     LDVEHLADDDDIATVAGLVISANGHIPRVGDVIDVGPLHITIFEANDYRVDLVRIVKE
                     QPAHDEDE"
     gene            complement(4915..6513)
                     /locus_tag="OO357_002730"
     CDS             complement(4915..6513)
                     /locus_tag="OO357_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707307.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5887623.1"
                     /translation="MQKAQRIIKTYRRNRMIVCTICALVTLASTLSVRFISQRNLNQQ
                     RVVQFANHAVEELDKVLLPLQAGSKVLLPLIGLPCSVAHLPLRKQAAKLQTVRSIGLV
                     QDGTLYCSSIFGYRNVPVVDILAELPAPQPLLRLTIDRALIKGSPVLIQWTPAAGSSN
                     AGVIEMINIDLLTAMLLEPQLPQISSASLTVDKRHLLYGNGLVDSLPQPEDNENYQVS
                     SQSFPFTINVNGPGATALAWHYLPTQLPLAVLLSLLVGYIAWLATAYRMSFSREINLG
                     LAQHEFELFCQPLLNARSQQCIGVEILLRWNNPRQGWISPDVFIPIAEEHHLIVPLTH
                     YVMAETIRQHHVFPMSSQFHVGINVAPSHFRRGVLIKDLNQYWFSAHPIQQLILEITE
                     RDALLDVDYRIARELHRKNVKLAIDDFGTGNSSFSWLETLRPDVLKIDKSFTAAIGSD
                     AVNSTVTDIIIALGQRLNIELVAEGVETQEQAKYLRRHGVHILQGYLYAQPMPLRDFP
                     KWLAGSQPPPARHNGHITPIMSLR"
     gene            complement(6644..8008)
                     /gene="sdaA"
                     /locus_tag="OO357_002731"
     CDS             complement(6644..8008)
                     /gene="sdaA"
                     /locus_tag="OO357_002731"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HCR5887624.1"
                     /translation="MISLFDMFKVGIGPSSSHTVGPMKAGKQFVDDLVEKGLLDSVTR
                     VAVDVYGSLSLTGKGHHTDIAIIMGLAGNEPATVDIDSIPGFIRDVKERERLLLAQGR
                     HEVDFPRDNGMRFHNGNLPLHENGMQIHAYNGDEVVYSKTYYSIGGGFIVDEEHFGQD
                     AANEVSVPYPFKSATELLAYCNETGYSLSGLAMQNELALHSKKEIDEYFAHVWQTMQA
                     CIDRGMNTEGVLPGPLRVPRRASALRRMLVSSDKLSNDPMNVIDWVNMFALAVNEENA
                     AGGRVVTAPTNGACGIVPAVLAYYDHFIESVSPDIYTRYFMAAGAIGALYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGGSPEQVCVAAEIGMEHNLGLTCDPVAGQVQVPC
                     IERNAIASVKAINAARMALRRTSAPRVSLDKVIETMYETGKDMNAKYRETSRGGLAIK
                     VQCD"
     gene            complement(8192..8770)
                     /locus_tag="OO357_002732"
     CDS             complement(8192..8770)
                     /locus_tag="OO357_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HCR5887625.1"
                     /translation="MEYRSLTLDDFLSRFQLLRPQINREPLNHRQAAVLIPIVRRPQP
                     GLLLTQRSIHLRKHAGQVAFPGGAVDDTDASVIAAALREAEEEVAIPPSAVEVIGVLP
                     PVDSVTGYQVTPVVGIIPPDLPYRASEDEVSAVFEMPLAQALHLGRYHPLDIYRRGDS
                     HRVWLSWYEQYFVWGMTAGIIRELALQIGVTP"
     gene            complement(8774..10135)
                     /gene="pabB"
                     /locus_tag="OO357_002733"
     CDS             complement(8774..10135)
                     /gene="pabB"
                     /locus_tag="OO357_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate synthase component 1"
                     /protein_id="HCR5887626.1"
                     /translation="MKTLSPTVITLPWRQDAAEFYFSRLSHLPWAMLLHSGYADHPYS
                     RFDIVVADPICTLTTLGKETVVSESEKRTTTTDDPLQVLQQVLDRADIRPTHNEDLPF
                     QGGALGLFGYDLGRRFESLPEIAEQDIVLPDMAVGIYDWALIVDHQRHTVSLLSHNDV
                     NARRAWLESQQFSPQEDFTLTSDWQSNMTHEQYGEKFRQVQEYLHSGDCYQVNLAQRF
                     HATYSGDEWQAFLQLNQANRAPFSAFLRLEQGAILSLSPERFILCDNSEIQTRPIKGT
                     LPRLPDPQEDSKQAEKLANSAKDRAENLMIVDLMRNDIGRVAVAGSVKVPELFVVEPF
                     PAVHHLVSTITARLPEQLHASDLLRAAFPGGSITGAPKVRAMEIIDELEPQRRNAWCG
                     SIGYLSFCGNMDTSITIRTLTAINGQIYCSAGGGIVADSQEEAEYQETFDKVNKILRQ
                     LEK"
     gene            10209..10388
                     /locus_tag="OO357_002734"
     CDS             10209..10388
                     /locus_tag="OO357_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaH family protein"
                     /protein_id="HCR5887627.1"
                     /translation="MFAGLPSLIHEQQQKAVERIQELMAQGMSSGQAIALVAEELRAN
                     HSGELIVARFEDEDE"
     gene            complement(10508..10867)
                     /locus_tag="OO357_002735"
     CDS             complement(10508..10867)
                     /locus_tag="OO357_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001331208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1889 family protein"
                     /protein_id="HCR5887628.1"
                     /translation="MQIKVIYSLIDNMVNFKDKNMPAVIDKALDFIGAMDVSAPTPSS
                     MNESTAKGIFKYLKELGVPASAADITARADQEGWNPGFTEKMVGWAKKMETGERSVIK
                     NPEYFSTYMQEELKALV"
     gene            complement(11229..11573)
                     /locus_tag="OO357_002736"
     CDS             complement(11229..11573)
                     /locus_tag="OO357_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HCR5887629.1"
                     /translation="MTIVRIDAEARWSDVVIHNNTLYYTGVPENLDADAFEQTANTLA
                     QIDAVLEKQGSNKSSILDATIFLADKNDFAAMNKAWDAWVVAGHAPVRCTVQAGLMNP
                     KYKVEIKIVAAV"
     gene            11705..13615
                     /locus_tag="OO357_002737"
     CDS             11705..13615
                     /locus_tag="OO357_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HCR5887630.1"
                     /translation="MTDDFAPDGQLAKAIPGFKPREPQRQMAVAVTQAIEKGQPLVVE
                     AGTGTGKTYAYLAPALRAKKKVIISTGSKALQDQLYSRDLPTVSKALKYTGNVALLKG
                     RSNYLCLERLEQQALAGGDLPVQILSDVILLRSWSNQTVDGDISTCVSVAEDSQAWPL
                     VTSTNDNCLGSDCPMYKDCFVVKARKKAMDADVVVVNHHLFLADMVVKESGFGELIPE
                     ADVMIFDEAHQLPDIASQYFGQSLSSRQLLDLAKDITIAYRTELKDTQQLQKCADRLA
                     QSAQDFRLQLGEPGYRGNLRELLANPQIQRAFLLLDDTLELCYDVAKLSLGRSALLDA
                     AFERATLYRTRLKRLKEINQPGYSYWYECTSRHFTLALTPLSVADKFKELMAQKPGSW
                     IFTSATLSVNDDLHHFTSRLGIEQAESLLLPSPFDYSRQALLCVPRNLPQTNQPGSAR
                     QLAAMLRPIIEANNGRCFMLCTSHAMMRDLAEQFRATMTLPVLLQGETSKGQLLQQFV
                     SAGNALLVATSSFWEGVDVRGDTLSLVIIDKLPFTSPDDPLLKARMEDCRLRGGDPFD
                     EVQLPDAVITLKQGVGRLIRDADDRGVLVICDYRLVMRPYGATFLASLPPAPRTRDIA
                     RAVRFLAIPSSR"
     gene            13673..14368
                     /gene="tsaB"
                     /locus_tag="OO357_002738"
     CDS             13673..14368
                     /gene="tsaB"
                     /locus_tag="OO357_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HCR5887631.1"
                     /translation="MRILAIDTATEACSVALWNDGTVNAHFELCPREHTQRILPMVQD
                     ILTTSGTSLTDINALAYGRGPGSFTGVRIGIGIAQGLALGAELPMIGVSTLMTMAQGA
                     WRKNGATRVLSAIDARMGEVYWAEYQRDENGIWHGEETEAVLKPELVHERMQQLSGEW
                     VTVGTGWQAWPDLGKESGLVLRDGEVLLPAAEDMLPIACQMFAEGKTVAVEHAEPVYL
                     RNNVAWKKLPGKE"
     gene            14408..14989
                     /gene="yeaY"
                     /locus_tag="OO357_002739"
     CDS             14408..14989
                     /gene="yeaY"
                     /locus_tag="OO357_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Slp family lipoprotein YeaY"
                     /protein_id="HCR5887632.1"
                     /translation="MAVQKNVIKGILAGTFALMLSGCVTVPDAIKGSSTTPQQDLVRV
                     MSAPQLYVGQEARFGGKVVAVQNQQGKTRLEIATVPLDSGARPTLGEPSRGRIYADVN
                     GFLDPVDFRGQLVTVVGPITGAVDGKIGNTPYKFMVMQVTGYKRWHLTQQVIMPPQPI
                     DPWFYGGRGWPYGYGGWGWYNPGPARVQTVVTE"
     gene            15195..16880
                     /gene="fadD"
                     /locus_tag="OO357_002740"
     CDS             15195..16880
                     /gene="fadD"
                     /locus_tag="OO357_002740"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase FadD"
                     /protein_id="HCR5887633.1"
                     /translation="MKKVWLNRYPADVPTEINPDRYQSLVDMFEQSVARYADQPAFVN
                     MGEVMTFRKLEERSRAFAAYLQQGLGLKKGDRVALMMPNLLQYPVALFGILRAGIIVV
                     NVNPLYTPRELEHQLNDSGASAIVIVSNFAHTLEKVVDKTAVQHVILTRMGDQLSTAK
                     GTVVNFVVKYIKRLVPKYHLPDAISFRSALHNGYRMQYVKPELVPEDLAFLQYTGGTT
                     GVAKGAMLTHRNMLANLEQVNATYGPLLHPGKELVVTALPLYHIFALTINCLLFIELG
                     GQNLLITNPRDIPGLVKELAKYPFTAITGVNTLFNALLNNKEFQQLDFSSLHLSAGGG
                     MPVQQVVAERWVKLTGQYLLEGYGLTECAPLVSVNPYDIDYHSGSIGLPVPSTEAKLV
                     DDDDNEVPPGQPGELCVKGPQVMLGYWQRPDATDEIIKNGWLHTGDIAVMDEEGFLRI
                     VDRKKDMILVSGFNVYPNEIEDVVMQHPGVQEVAAVGVPSGSSGEAVKIFVVKKDPSL
                     TEESLVTFCRRQLTGYKVPKLVEFRDELPKSNVGKILRRELRDEARGKVDNKA"
     gene            16962..18077
                     /gene="rnd"
                     /locus_tag="OO357_002741"
     CDS             16962..18077
                     /gene="rnd"
                     /locus_tag="OO357_002741"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HCR5887634.1"
                     /translation="MITTDDALASLCEAVRVFPAIALDTEFVRTRTYYPQLGLIQLFD
                     GEHLALIDPLGITDWSPLKAILRDPSITKFLHAGSEDLEVFLNVFGELPQPLIDTQIL
                     AAFCGRPMSWGFASMVEEYSGVTLDKSESRTDWLARPLTERQCEYAAADVWYLLPITA
                     KLMVETEASGWLPAALDECRLMQMRRQEVVAPEDAWRDITNAWQLRTRQLACLQLLAD
                     WRLRKARERDLAVNFVVREEHLWSVARYMPGSLGELDSLGLSGSEIRFHGKTLLALVE
                     KAQTLPEDALPQPMLNLMDMPGYRKAFKAIKSLITDVSETHKISAELLASRRQINQLL
                     NWHWKLKPQNNLPELISGWRGELMAEALHNLLQEYPQ"
     gene            complement(18131..19096)
                     /gene="yeaX"
                     /locus_tag="OO357_002742"
     CDS             complement(18131..19096)
                     /gene="yeaX"
                     /locus_tag="OO357_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaX"
                     /protein_id="HCR5887635.1"
                     /translation="MSDYQMFEVQVSQVEPLTEQVKRFTLVATDGKPLPAFTGGSHIL
                     VQMSDGDNQYSNAYSLLSSPHNTSSYQIAVRLEENSRGGSRFLHQQVKVGDRLTISTP
                     NNLFALIPSARKHLFIVGGIGITPFLSHMAELQHSDIDWQLHYCSRNPESCAFRAELV
                     QHPQAEKVHLHHSSTGTRLELARLLADIEPGTHVYTCGPEALIEAVRSEAARLDIAAD
                     TLHFEQFAIEDKTGDAFTLVLARSGKEFVVPEEMTILQVIENNKAAKVECLCREGVCG
                     TCETAILEGEADHRDQYFSDEERASQQSMLICCSRAKGKRLVLDL"
     gene            complement(19152..19769)
                     /gene="yeaW"
                     /locus_tag="OO357_002743"
                     /pseudo
     CDS             complement(19152..19769)
                     /gene="yeaW"
                     /locus_tag="OO357_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310538.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="carnitine monooxygenase subunit YeaW"
     gene            complement(20111..>20614)
                     /locus_tag="OO357_002744"
     CDS             complement(20111..>20614)
                     /locus_tag="OO357_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005098561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5887636.1"
                     /translation="RAFAAKCGLSDGVIRNYLSGKTYPSLDRLAQIAYATGRPIEWFI
                     QSQSMPMVEHKNNQENKNSQPDRSTLKTKLIAIVELLEDKELHSAIELFRKKGFEALM
                     PEIFSSDDSIQADLQGISQQTLQTAKMLESLPTDTRKKILSKYGIYEQEGLVAPSQEP
                     QDVKKAV"
BASE COUNT         5069 a         5134 c         5335 g         5076 t
ORIGIN      
        1 taacgctatc gatgcgacca cgccggagca aaggaattgc cagcagggtg gggccgttac
       61 ggcggggcat gatgaactga tcgtagatcg cgaaggccag gagtgcggcg atgaaaagaa
      121 tcagtaccag gtccgtgatt gtcattaatc ctccagataa aaaaacgggg ccaaaaggcc
      181 ccggtagtgt acgacagtct tacagtccca gcaggccgca agcgtaacca gcgataccga
      241 tgacgaagaa gccaacgatg atccacagcg ggttaacttt tttgcgcagc agccacatac
      301 aagcaaatgt cagcagcagt ggtacaaggc ctggcattaa ctggtcaaga atagtttgca
      361 cggtggtgac acgggtctgt ccatcctgcc cggtgatggt cgaaaccacc agcgggatgt
      421 tgacatgtgt ccacttgtta accaatgccc ccatgacaaa caggccgagg atagacgccc
      481 cttccgtcag tttttgcagg aagccaccac ccatatcttt aacgatatcg atacctttgg
      541 agtaaccata cgctacgccg tagtaacggg ttgccagacg caccaggtta aacaggatga
      601 agaacagcag cggacctaac aggctgccgc tcatcgcgat accggcaccc agtgctgcaa
      661 ataccggacg tacggttccc cagaagatcg ggtcgcctac accagccagc ggccccatca
      721 aaccgacttt gataccgttg atagcaccgt catcgatctc tgcgccatta gcacgctgtt
      781 cttccagcgc cagggttacg ccgagaatcg gtgcagccac gaacggctgg gtgttaaaga
      841 actccaggtg acggcgaata gcttgtttac gagcttcgtt gttctcaggg tagaggcgac
      901 gaattgccgg taccatagag aagcagaaac ccagtgcctg catacgttcg aagttccatg
      961 aaccctggaa gaggttagaa cgcaggaata cgccacgaat atcactttga gtgagttttt
     1021 tctcggtggt agtttgagtt gtatcaacca tttcgctcac ctgttagtcc agttcgttat
     1081 cgagatcgtt gttaccagct gcctgagcag gcgcaccggc tacgcggttg tatttcgggc
     1141 taagttggat gtagagcact gccataacag taccaatcac acccagagca accaggttaa
     1201 agttggtgaa tgctgcggtt acgaagccga ggtagaagaa cggcatcagg tagccagcac
     1261 gcatcatgtt gataaccatc gcataaccaa ccaccacgat catgcccccg gcgatattca
     1321 gaccattggt caccacttcc ggaatcgcat tcagcatgtt ctgtacttcg ctggtaccaa
     1381 cagacagcgc aacgatgacg gccggaatag ccacacgcat tgcttgcagg aacagagaag
     1441 aaacgtggat ccaggaaatc gctgtcaggt tgccgttatc agcagcctta tccgcagcgt
     1501 gctggaaagc aacggtaata gtacgaacga tgatggtcag tacctggccc gcagcggcca
     1561 gagggattgc cagtgcgata cctgcaccaa tgctctgatg acctgcgata accagaatgg
     1621 tagaaatgat agaagccaga gcggcgtcag gcgcaactgc agcaccgatg ttcatccagc
     1681 ccagcgcgat catttccagc gtaccaccga taataatacc ggttttcata tccccaagaa
     1741 cgatacccac cagggtacac gcgattagcg gacggtgaaa ctgaaattca tcgaggattg
     1801 atcccatacc tgcgatacag gctacgataa ataccagcac aatttgaaga gtggtaatct
     1861 ccattgtact tctcctgttt acgtaagact taagtgtgaa aactgaatga taatcaacac
     1921 aatacgttac ttatcgattt tgctgatcag atccatcatt ttcagtttcg gatcggtgga
     1981 aaccttacgg acttccagct caataccgcg tgcattcagt tttttgaacg cctcgatatc
     2041 tttttcatca accgaaaccg cgttattcac ctgggtttta ccctgacgga atgccatacc
     2101 accgacgtta acagaggtga ttttcacgcc gccttcaacg agacgctcta catctgttgg
     2161 gttggtaaat aacagcatta cgcgttcgcc agcatatttc gggttgttgt agacgcgaat
     2221 cattttggca acatcaacta cgtgtgctgt tacgcccgga ggtgcaacct gggtgagcag
     2281 tgtcttacga acggtatccg cagccacttc atcactaaca acaataatac gggagacatt
     2341 ggtttctttg gtccagcggg tggcgacctg accgtgaatc agacggtcgt cgatacgcgc
     2401 aaggccaata accatgtagt cgtttggccc cattggtttt gccggagttg gagccgcttt
     2461 tggtgctgcg gcagcgggtg ctggcgcggc tttttcaacc ggtttggctt tcagtgcttt
     2521 cacgccttca cggcctgttt ctactgccag cgcaaccagt tcatcaaagc tcgggtcatc
     2581 atcacgggcc attaacgttt ccacgagcat tggaatgtta acgcctgcaa tgacttcata
     2641 atgctctttg tcgacgacaa tgcggctggc agcattgaac gggctgcctc cccatgtatc
     2701 aacgagaaac agcacgcctt tagtggtgtc gagttttgcc aactgagcgt tgtatttttc
     2761 aatcagcgtt tcggcatttt cacctggaac gaaatcgatc cagccgacgt tttcctgctc
     2821 gcctaacagc atttctgccg ttttaagcaa ctgctctgca gcccaaccat gtgtgcctat
     2881 aacaatagca atggtcactt gctacctcct ttattatcgt taacacctca acgtgccaga
     2941 tgtatttttg aatcgtcgtc cacaatcgaa tcgattcaga taagggctac aaccaaaaac
     3001 ccctgcgttt cgcgatttat tttagatatc gaaaaaatta ttttatgtga tgaagatccg
     3061 taatttaact ttcgattatc agaaatttcg aaaggtaaaa tatccttgtc acattcgttt
     3121 gcaaaggaag gtaaatcttt gccaaatcag aggcgtctct gatatgttta actcccgttt
     3181 aacaaccatg gagtataggg cagtagcccg cagtatggat cgtcaccgac gtcatttcag
     3241 catcaggcct tttaacgcct gcctttctgg cactctatgc cgcacctttc gtttgcattt
     3301 tgtcgttacg cctgcattat ttctggcgtc gaatagctat tccttaagca ggagcttgtc
     3361 atggaattct taatggaccc ctcaatttgg gcggggctac tcacgcttgt tgttctcgaa
     3421 attgtgctgg gtatcgataa cctggtcttc atcgccattc ttgctgacaa actgccgcca
     3481 aaacaacgcg ataaagcgcg tttgctgggg ttatcactgg cgctgattat gcgtctgggg
     3541 ctgctgtcgc tgatttcatg gatggtcacg ctgaccaaac cgctatttac cgtcatggat
     3601 ttctccttct ccggacgcga tctgattatg ttgttcgggg ggatattctt gctgttcaaa
     3661 gcaacaaccg aactgcatga acggctggaa aaccgcgatc atgattccgg ccacggtaaa
     3721 ggctacgcca gtttctgggt ggtcgtcaca cagattgtca tccttgacgc cgtcttctcg
     3781 ttggatgcgg taattactgc agtagggatg gttaaccatc tgccggtgat gatggcggcg
     3841 gtagtgattg cgatggcggt tatgttgctg gcatcgaaac cgctgacgcg attcgttaac
     3901 cagcacccca cggtggtggt gctctgtctg agcttcctgt taatgattgg tctgagtctg
     3961 gtggcagaag gtttcggttt ccacattccg aaaggttacc tgtatgccgc gattggcttc
     4021 tcgatcatta tcgaagtgtt taaccagatt gcgcgtcgca actttattcg tcaccagtcg
     4081 actttgccgc tgcgagcgcg tactgccgat gccatcctgc gtttgatggg cgggaaacgt
     4141 caggccaatg ttcagcacga tgccgataac ccgatgccga tcccggaagg tgcatttgcc
     4201 gaagaagaac ggtacatgat taacggcgta ctgacgctgg cgtcgcgttc tctgcgcggg
     4261 atcatgacgc cgcgcggtga aataagctgg gttgacgcta atctcggggt cgatgaaatc
     4321 cgcgagcaac tgctctcttc accgcacagt ctgttcccgg tatgtcgcgg tgaactggat
     4381 gaaatcatcg gtatcgtacg tgctaaagaa ctgctggtgg cgctggaaga gggcgttgat
     4441 gtggcggcga ttgcttcggc gtctccggcg attatcgtcc cggaaaccct cgatccgatc
     4501 aacctgctgg gcgtgctgcg tcgcgctcgc gggagctttg ttatcgtgac caacgagttt
     4561 ggtgtggtac aaggcttggt cacgccgctg gatgtgctgg aagccattgc gggtgaattc
     4621 ccggacgctg acgaaacgcc ggaaatcatt accgatggta acggctggct ggtaaaaggc
     4681 ggtacagatt tgcatgcctt gcagcaggcg cttgatgttg agcaccttgc cgatgacgat
     4741 gatatcgcga cggtcgcggg cctcgtgatc tcggcaaatg gtcacattcc ccgtgtgggc
     4801 gatgtgattg atgtagggcc actgcatatc accatctttg aagccaatga ttatcgtgtt
     4861 gatctggttc gcattgttaa agagcaaccg gcgcacgatg aagatgagta agcgttaacg
     4921 taacgacata atgggcgtga tatgtccatt atgccgggcg ggcggcggtt ggctgcccgc
     4981 cagccatttg ggaaaatcac gtagcggcat cggctgtgcg tacaaatacc cttgcaaaat
     5041 atgcacgcca tgacggcgca aatacttcgc ctgttcttgc gtttccacgc cctccgccac
     5101 cagttcaata ttcagtcttt gccccagcgc gatgatgata tcggtcaccg tcgagttaac
     5161 cgcgtcagaa cctatagctg cggtaaatga cttatcaatt ttcagcacgt caggacgtaa
     5221 tgtttcaagc caggaaaacg agctgttgcc ggtgccgaag tcatcaatcg ccagtttgac
     5281 gtttttacga tgcagctcgc gggcaatccg ataatcaaca tccagtaagg catcgcgttc
     5341 ggtgatttcg aggatcagtt gctgaatcgg gtgagcgcta aaccagtact gattgagatc
     5401 ttttatcagc acaccacggc gaaaatggct gggtgcgacg ttaatgccaa catgaaactg
     5461 actactcatc gggaaaacat ggtgctgacg aatggtttct gccatcacat agtgggtcag
     5521 tggcacaatt aaatgatgtt cttccgcgat aggaataaac acatccggtg aaatccagcc
     5581 ctgacgcgga ttattccagc gcagcaaaat ctctacacca atacattgct ggctgcgcgc
     5641 attaagcaaa ggctgacaga acaattcgaa ctcatgttgc gccaggccca gattaatttc
     5701 gcgggaaaag ctcatccggt aagcggtcgc cagccaggcg atatagccca ccagtaaact
     5761 tagcagcacc gccagcggta attgtgttgg aagatagtgc catgccagcg ccgtagcccc
     5821 cggaccattt acgttaatgg taaaaggaaa gctttgcgaa gaaacctgat agttttcatt
     5881 gtcttcaggt tgcggaaggg aatctaccag cccattacca tagagcaaat gccgtttgtc
     5941 caccgtcagg ctggcgctac tgatttgcgg cagttgtggc tcaaggagca ttgccgtcag
     6001 taagtcgatg ttaatcatct ctatgacccc agcattgcta ctgcccgctg caggcgtcca
     6061 ttgaatcaaa accggactgc ctttaatcag ggcacggtcg atcgtcaggc gtaaaagtgg
     6121 ttgcggtgca ggaagttcag ccagaatgtc cacgacgggc acattgcgat aaccaaaaat
     6181 gctggagcaa taaagtgtgc cgtcttgcac caggccaatg gatcgcacag tttggagttt
     6241 tgccgcctgt ttacgtaatg gcaaatgggc gacagagcag ggcagaccaa tcagcggaag
     6301 caagactttg ctacctgcct gtaggggaag cagtacttta tctaattcct ctacagcgtg
     6361 attggcgaat tgtactaccc gttgttgatt taagttacgc tgtgaaataa atcgcacgct
     6421 cagggtcgaa gcgagcgtaa cgagtgcgca aatcgtacaa acaatcattc gattacggcg
     6481 ataggtttta atgatccgtt gtgctttttg catgcgggcc accctgtaag cgtatggcga
     6541 caaacgcctg acgcaacagt ggaagtgtag tggggaatat tacagcagac gagaaagcgg
     6601 gtataaattc gcccatccgt tgcagatggg cgagtaagaa gtattagtca cactggactt
     6661 tgattgccag accaccgcgt gaggtttcgc ggtatttggc gttcatgtcc ttaccggttt
     6721 cgtacatcgt ttcgatgacc ttatccagcg agacgcgcgg tgcactggtg cggcgcagag
     6781 ccatccgtgc ggcgttaatc gccttcacag aggcaatggc attacgctca atgcacggca
     6841 cctgaacctg ccctgcaacc gggtcgcagg ttaaaccaag gttgtgttcc atgccaattt
     6901 ccgccgccac gcaaacctgt tccgggctac cgcccagcag ttctgcaaga cccgcagcag
     6961 ccattgaaca ggcaacaccc acttcgccct ggcaaccaac ttccgcaccg gaaatagagg
     7021 cgttcatttt atacaatgca ccaatcgcgc ccgctgccat aaagtaacgg gtatagatgt
     7081 ctgggctgac cgattcaata aagtggtcat agtaagccag cactgccgga acgataccgc
     7141 aggcaccgtt ggttggcgca gttaccacac gaccaccggc ggcgttttct tcgttaactg
     7201 ccagcgcaaa catgtttacc cagtcaatga cattcatcgg atcgttagac agtttatcgc
     7261 tggaaactaa catccggcgc agggcagacg cacgacgtgg cacgcgcagc gggcctggca
     7321 gtacaccttc ggtgttcatc ccgcgatcga tacatgcctg catggtttgc cagacatgcg
     7381 cgaaatactc gtcgatctct ttcttgctgt gcagcgccag ttcgttctgc atagcgagac
     7441 cagacagcga atagccggtt tcattacagt acgcgagcag ttcggtggca gatttgaacg
     7501 gatacggcac gctcacttcg ttggcagcat cctgaccaaa gtgttcttca tcgacgataa
     7561 aaccgccgcc gatggaataa taagttttgc tgtagacgac ttcatcgccg ttataggcgt
     7621 ggatttgcat accgttttca tgcagcggca ggttgccgtt atgaaaacgc atcccgttgt
     7681 cgcgcgggaa atccacttca tgccgtccct gtgccagcag cagacgttcg cgctctttta
     7741 cgtcgcgaat aaaaccggga atactgtcga tatccacggt ggcaggttcg ttacctgcaa
     7801 gacccataat aatggcgata tcggtgtggt ggcctttacc cgtcagcgac agtgaaccat
     7861 aaacgtccac ggcaacgcga gtaacgctat ccagtaagcc tttttcgacc agatcatcga
     7921 cgaactgttt acccgccttc ataggcccta cggtatggga agatgaggga ccaatcccca
     7981 ccttaaacat gtcgaataga ctaatcacga taatactcct gacaaggtga ctggacttcc
     8041 agtaacgaac taataacagc gcatagtgta agagggaacg cggcgactgg cttaactatt
     8101 cacatgaatt aaactaatca aaaaccgcga tttaactaat attgcgatga ttgtctcaag
     8161 tagtgttttg tagatgtaaa gataagtata gtcagggggt cacaccaatt tgcagcgcca
     8221 gctcacgaat tatgcctgcg gtcattcccc atacaaaata ctgttcgtac caggacagcc
     8281 atacccgatg tgaatcgcca cggcggtaga tatctaaagg gtgataacga cccagatgta
     8341 atgcctgggc gagcggcatt tcaaacaccg ccgagacttc atcttcactg gcgcgatacg
     8401 gcagatcggg cgggataatg ccgaccactg gggttacttg atagccagtt acgctatcga
     8461 cgggcggcag cacgccgata acttcaacgg cggaaggcgg tatagcgacc tcttcttcag
     8521 cttcgcgcag cgcggcggcg ataactgatg cgtccgtgtc atcgactgca cctccaggga
     8581 atgccacttg tccagcgtgt ttacgcagat gaattgaacg ctgagtcagc aacaaccccg
     8641 gttgcggtcg acggacgatg gggattaaca cggcagcctg acgatgattt aggggttccc
     8701 ggttaatttg cgggcgcaaa agttgaaagc gagataaaaa atcatcaagc gtcaggctac
     8761 ggtattccac gtcttacttc tccagttggc gtaatatctt attaacttta tcaaaagttt
     8821 cctgatattc cgcttcttcc tggctatcgg cgacaattcc accgcccgca gagcagtata
     8881 tttgtccgtt aatggcagtc agcgtgcgga tagtaatgct ggtatccatg ttgccgcaaa
     8941 agctcaaata gccaatgctg ccgcaccagg cattacgtcg ctgcggttcc agttcgtcga
     9001 taatttccat agcccgtact ttcggagccc cggttattga gccaccagga aaagctgcgc
     9061 gcagcagatc gctggcgtgt aactgttctg gtagtcgcgc cgttatagtg ctgaccagat
     9121 gatgcacggc agggaagggt tccaccacga agagctctgg tacttttacc gaaccggcta
     9181 cggcaacacg accgatatca ttacgcatta aatcgacaat catcagattt tcggcacgat
     9241 ctttcgctga gttcgccagt ttttctgctt gtttgctatc ttcctgagga tcgggcaggc
     9301 gtggtagcgt gcctttaatc gggcgagtct ggatttcact attatcacaa agaataaacc
     9361 gctctggcga aaggcttaaa attgcaccct gttcaagacg taaaaaagcg ctaaatggcg
     9421 cgcggttggc ctgattaagc tgaaggaatg cctgccattc atcgccagaa taggtcgcat
     9481 gaaaacgctg ggcgagattc acctgatagc aatcaccgct gtgcagatat tcctgtacct
     9541 ggcgaaattt ttcgccgtac tgctcgtggg tcatattgga ttgccagtcg gaagtgagcg
     9601 tgaaatcttc ctgcggcgag aattgctggc tttccagcca ggcccgacgg gcattgacat
     9661 cattatgact cagcaaagaa actgtatgac gctggtggtc gacaatgagc gcccaatcgt
     9721 agatacccac tgccatatcc ggcagaacga tatcttgttc cgcaatttct ggcagtgact
     9781 caaaacggcg gcccagatcg tagccaaaca accccagtgc gccgccctga aatggcaaat
     9841 cttcgttatg cgttgggcga atgtctgcgc gatccagcac ctgctggagc acctgtagcg
     9901 ggtcatcagt ggtcgttgtg cgtttttcgc tttcactaac aacggtttct ttacctaaag
     9961 tggttaaagt gcaaatcgga tcggcgacca caatatcaaa gcggctatac ggatgatcgg
    10021 catagccgga gtgtaaaagc atcgcccacg gcaggtggct taagcgggag aaataaaatt
    10081 cagcggcgtc ctgacgccag ggtaaagtaa tcacagtggg agataacgtc ttcatcagtc
    10141 ctgactctac tggctatgtg ccgggtagca tgaaataatt agcgccaaca atttagcagg
    10201 agttaacaat gtttgcaggt ttaccttcac tcatccatga acagcagcaa aaagctgtcg
    10261 agcggatcca ggaactgatg gcacaaggga tgagtagcgg ccaggcaatc gcgctggtgg
    10321 cagaagagct gcgtgccaac cactccggcg agctaatcgt ggcgcgtttt gaggatgaag
    10381 acgagtaaca cactaatccg tatcgatttc gttatagaag ccgatgatca tttgattgtg
    10441 agaaaaagga cgctttttat atgtgattaa aagcgtcctg acccatttat gctgatataa
    10501 atttaactca gaccagtgct tttagttctt cctgcatata tgttgaaaag tattcaggat
    10561 ttttaatcac agagcgttca cctgtctcca ttttttttgc ccatccaacc attttttccg
    10621 tgaacccagg gttccagcct tcctggtcag ctcgcgccgt aatatcagcg gcactcgcgg
    10681 gtactcccag ttcttttaaa tatttaaaga ttcccttcgc cgtgctttca ttcatcgaac
    10741 ttggtgttgg cgctgataca tccatggcac caatgaaatc cagggcttta tctattacag
    10801 caggcatatt cttatcctta aaattaacca tgttatcgat aaggctatat atgaccttaa
    10861 tttgcatcta atatcaaagg gattataacg atatggcacg agttaacctg ttgacattca
    10921 ggtgttttaa aggtagcagc tcatggtgtg ttatccagta agatggagaa aattaacggt
    10981 cagcccgcac gctataacca gattgttcag ctaagaaaga aaaccagagt tgctaatcaa
    11041 agggcgaaca caacggcatg gttatgttta tttatatagt tagttttata gataaaattg
    11101 aaattataaa tataaataat gaattagcag aagcatcaac agtaatatct tattctctgg
    11161 tcgatttatg taaaaccgac cagtattatc gataaattat cgtctgactt attttgcttc
    11221 gataaagctt acaccgcagc cacaatctta atttcaactt tatacttcgg gttcatcaaa
    11281 cccgcttgta ccgtgcagcg caccggcgca tgacccgcga caacccaggc atcccacgct
    11341 ttattcatcg ccgcgaagtc gtttttatcg gccaggaaaa tggtggcatc cagaatgctc
    11401 gatttattgc tgccctgttt ttccagcacg gcgtcaatct gtgccagcgt gttggcggtt
    11461 tgctcaaagg catcggcgtc gaggttttcc ggtacaccag tgtagtagag cgtgttgttg
    11521 tggattacta catcagacca gcgggcttca gcatcgatac gaacgatagt catcattttg
    11581 tcctcattat acttccattt tcgtgctgca aagactgcca caatgtgagg ttgccgtcac
    11641 cacttcaact ggcgaaagcg ccctcatcct gacataatcc ctgttcaaat caacaggggg
    11701 tagtgtgacg gacgattttg caccagacgg tcagctggcg aaagcgatac caggctttaa
    11761 gccgcgagaa ccacagcgac agatggcggt agccgtcacc caggcgatag aaaaaggcca
    11821 gccgctggtg gtggaagcag gaaccggtac gggcaaaacc tacgcttacc tggctcctgc
    11881 gctgcgggcg aaaaagaaag tcattatctc gaccggctca aaagcgttgc aggatcagct
    11941 ctacagccgc gatttgccaa cagtctcaaa ggcattgaaa tatacgggca acgtggcgct
    12001 gctgaaaggg cgctcaaact acctctgcct cgaacgtctc gaacagcagg cgctggcggg
    12061 gggcgatctg ccggtacaaa tcttaagcga tgtgatcctg ctgcgctcct ggtctaatca
    12121 aacagtcgat ggtgatatca gcacctgcgt cagcgtggcg gaagattcac aggcgtggcc
    12181 gctggtcacc agcaccaacg acaactgtct tggcagcgac tgcccgatgt ataaagattg
    12241 ctttgtggtc aaagcacgta aaaaagcgat ggacgccgat gtggtggtgg taaaccatca
    12301 tctctttctg gcggatatgg tggttaaaga gagtggattt ggcgagttga tccctgaagc
    12361 ggacgtcatg atcttcgacg aagcccacca gctaccggac attgccagcc agtattttgg
    12421 tcagtcactc tccagtcgac aactgctcga cctggcaaaa gacatcacca tcgcctaccg
    12481 caccgaatta aaagacaccc agcagttaca aaagtgcgct gatcgtcttg cccagagtgc
    12541 gcaggatttt cgtctgcaac tcggtgagcc aggttatcgc ggtaacctgc gtgagctgtt
    12601 agctaatccg caaattcagc gggcattttt actgctcgat gacaccttgg aactttgtta
    12661 tgatgtggcg aaactgtcac tggggcgttc cgccttgctg gatgcggcat ttgagcgcgc
    12721 cacgttgtat cgcacacggc tgaagcggct aaaagagatc aatcagccgg gctacagcta
    12781 ctggtacgaa tgcacttcgc gccattttac tctggctctc acgccgctca gcgtggcgga
    12841 taaattcaaa gagttaatgg cgcaaaagcc cggtagctgg atcttcacct cagcaacgct
    12901 gtcggtgaac gacgatctgc atcatttcac ctcgcggctt gggatagaac aggcggagtc
    12961 gttgctattg ccaagcccgt ttgattacag ccgccaggcg ttactctgtg tgccgcgcaa
    13021 tctgccgcaa accaaccagc caggttctgc tcgccagtta gcggcaatgc tgcgaccgat
    13081 catcgaagct aacaacggtc gttgttttat gctttgtacc tcgcacgcca tgatgcgcga
    13141 tctggccgag cagttccgcg ctaccatgac gcttcccgtt ttgttgcagg gggaaaccag
    13201 taaaggccaa ctgttgcagc agtttgtcag tgccggtaat gcgcttcttg tggcaaccag
    13261 tagtttctgg gaaggggtgg acgtgcgtgg cgatacattg tcattggtaa ttatcgacaa
    13321 attgccgttt acctcgccgg atgatccact gttaaaagcg cgcatggaag attgtcgttt
    13381 gcgcggtggc gacccgttcg atgaagtgca actaccagat gccgtcatta ctctcaaaca
    13441 gggggtaggt cgactgattc gcgacgccga cgatcgtggc gtgctggtga tttgtgacta
    13501 tcggctggtg atgcgtcctt acggcgcgac gtttctcgcc agtctgccgc ccgcgccacg
    13561 cacccgtgac attgcccgtg cggttcgttt ccttgcgata ccatcctcca ggtaatttgt
    13621 tacggagtat ggtatgatgc gcgccttttt attgatctaa gcacgaagac ccatgcgaat
    13681 tctggctatc gataccgcga cagaggcctg ctctgtcgcc ctgtggaacg acggtactgt
    13741 caacgctcat tttgagcttt gccctcgtga acatactcaa cgaatcttac cgatggtgca
    13801 ggatatcctg accaccagcg gaacttccct gactgatatt aacgctctgg cttacgggcg
    13861 cggccccggt agctttaccg gcgtgcgcat tggtattggc atcgcgcaag ggctggcgct
    13921 tggcgcggaa ttaccgatga ttggcgtctc cacgctaatg acgatggcgc aaggggcgtg
    13981 gcgcaaaaac ggcgcaacac gcgtgctgtc agccattgat gcgcgaatgg gcgaagttta
    14041 ctgggccgaa tatcagcgtg atgaaaacgg tatctggcac ggtgaagaaa ccgaagccgt
    14101 actcaaaccc gaactcgttc atgaacgaat gcaacagctt tccggcgaat gggtgacggt
    14161 agggacgggc tggcaagcct ggccggatct cggtaaagag agcgggctgg ttttgcgcga
    14221 tggcgaagtg ttactccccg ctgcagaaga tatgctgccg attgcttgtc agatgtttgc
    14281 cgagggtaaa acggtggcgg tggaacatgc cgagccggtt tatttgcgta acaacgtcgc
    14341 atggaagaaa cttccgggca aagaatgaat cttaagagta tgccctgaga aaaaaaggag
    14401 tcgcatcatg gcggttcaaa agaatgttat caaaggcata ctggcaggta cgtttgcgct
    14461 aatgctgagc ggttgtgtca ctgtgccgga cgccattaaa ggcagcagta ccacgccgca
    14521 acaagattta gttcgggtga tgagtgcgcc gcagctgtac gttggtcagg aggcacgctt
    14581 tggtggcaaa gtggttgcgg tacaaaacca gcaagggaaa acccgcctgg aaattgctac
    14641 cgtaccgctg gacagcggag ctaggccgac gctgggagaa ccttctcgcg gtcgcattta
    14701 tgccgatgtg aacggttttc tggacccggt ggatttccgt ggacaactgg ttacggtagt
    14761 cgggccaatc accggtgcgg ttgacggcaa aatcggcaat acgccctata aatttatggt
    14821 gatgcaagta acgggttaca aacgttggca tttaacccag caggtgatta tgccgcctca
    14881 gccgattgat ccatggtttt atggcggtcg tggctggccc tatggctacg gcggatgggg
    14941 ctggtataat cccggccccg cgagagtaca aacagttgta accgaataat tgcttgtttt
    15001 taaagaatgc agaaacagcg gctggtccgc tgtttctgca ttcttacggt aaagataaaa
    15061 ataaatagtg acgcgcttcg caaccttttc gttgggtaat tatcaagctg gtatgatgag
    15121 ttaatattat gttaacggcg tgtatatcat ttggggttgc gatgacgacg aacacgcatt
    15181 ttagaggtga agaattgaag aaggtttggc ttaaccgtta tcccgcggac gttccgacgg
    15241 agatcaaccc tgaccgttat caatctctgg tagatatgtt tgagcagtcg gtcgcgcgct
    15301 acgccgatca gcctgcgttt gtgaatatgg gggaggtgat gaccttccgc aagctggaag
    15361 aacgcagtcg cgcgtttgcc gcttatttgc aacaagggtt ggggctgaag aaaggcgatc
    15421 gcgttgcgtt gatgatgcct aacttattgc aatatccggt ggcgctattt ggcattttgc
    15481 gtgccgggat aatcgtcgta aacgttaacc cgttgtatac cccgcgtgag cttgagcatc
    15541 agcttaacga tagcggcgca tcggcgattg ttatcgtgtc taactttgcc cacactctgg
    15601 aaaaagtggt tgataaaacc gccgttcagc acgtaattct gacccgtatg ggcgatcagc
    15661 tatctacggc aaaaggcacg gtagtcaatt tcgttgttaa atacatcaag cgcttggtgc
    15721 cgaaatacca tctgccagat gccatttcat ttcgtagcgc actgcataac ggctaccgga
    15781 tgcagtacgt caaacccgaa ctggtgccgg aagatttagc cttcctgcaa tacaccggcg
    15841 gcaccacagg tgtggcgaaa ggcgcgatgc tgactcaccg caatatgctg gcgaacctgg
    15901 aacaggtcaa cgccacctat gggccgttgc tgcatccagg taaagaactg gtagtaacgg
    15961 cactgccgct gtatcacatt tttgccctga ccattaactg cctgctgttt atcgaactgg
    16021 gtgggcagaa cctgcttatc actaacccgc gtgatattcc agggttggta aaagagttag
    16081 cgaaatatcc gtttaccgct atcactggcg ttaacacctt gttcaatgcg ttgctgaaca
    16141 ataaagagtt ccagcagctg gatttctcca gtctgcatct ttccgcaggc ggtgggatgc
    16201 ctgttcagca agtggtggca gaacgctggg tgaaactgac cggacagtat ctgctggaag
    16261 gttatggcct gaccgagtgc gcgccgctgg tgagcgttaa cccgtatgat attgattatc
    16321 atagcggtag catcggtttg ccggtgccat cgacggaagc caaactggtg gatgacgacg
    16381 acaacgaagt accaccgggt caaccgggtg agctttgtgt caaaggaccg caggtgatgc
    16441 tgggttactg gcagcgtccg gatgctacag atgagatcat caaaaatggc tggttacaca
    16501 ccggcgacat cgcggtgatg gatgaagaag gattcctgcg cattgtcgat cgtaaaaaag
    16561 acatgatttt ggtttccggt tttaacgttt atcccaacga gattgaagat gtcgtcatgc
    16621 agcatcctgg cgtacaggaa gtcgcggctg ttggcgtacc ttccggctcc agtggtgaag
    16681 cggtgaaaat cttcgtagtg aaaaaagatc catcgcttac cgaagagtca ctggtgactt
    16741 tttgccgccg tcagctcacg ggatacaaag taccgaagct ggtggagttt cgtgatgagt
    16801 taccgaaatc taacgtcgga aaaattttgc gacgagaatt acgtgacgaa gcgcgcggca
    16861 aagtggacaa taaagcctga gcgttaagtc agtcgtcaga cgccggttaa tccggcgttt
    16921 tttttgacgc ccactaaaga gaaaacaatt tgaattacca aatgattacc acggacgatg
    16981 cgctggcttc tttgtgtgaa gccgtccgtg tctttccggc gatagccctg gatactgaat
    17041 ttgttcgtac gcgcacttat tacccgcagc tggggttgat tcaacttttc gatggcgagc
    17101 atctggcgct aattgatcct ctcgggatca ccgactggtc accgctgaaa gcgatcctgc
    17161 gcgatccgtc catcacaaaa tttctccatg caggcagtga agatctggaa gtgttcctca
    17221 atgtctttgg cgaattacca caacccttga ttgacacgca aatccttgct gccttctgcg
    17281 gacgcccgat gtcatggggt ttcgcttcca tggtggaaga gtattccggc gttacgctgg
    17341 acaagagtga atcgcgcacc gactggctgg ccagaccgct gaccgaacgt cagtgtgaat
    17401 acgcagcggc ggatgtctgg tatctgttac cgatcaccgc caagcttatg gtagaaacgg
    17461 aggcctccgg ctggctacct gcggcgctgg atgaatgccg cctgatgcaa atgcgtcgtc
    17521 aggaagtcgt tgcgccggaa gatgcctggc gtgatatcac caatgcctgg cagttacgca
    17581 cacgccaact ggcctgtctg caactgttag ccgactggcg actgcgcaag gcgcgagagc
    17641 gtgatctggc ggtgaacttt gtcgtgcgtg aagagcattt gtggtcggtc gcgcgttata
    17701 tgccgggaag tttaggcgaa ctggacagcc tgggtttatc cggtagcgaa atccgctttc
    17761 acggtaaaac gctgctggcg ctggtggaaa aagcgcagac attgccggaa gatgccttac
    17821 cgcagccgat gcttaacctg atggacatgc ctggttatcg taaagcgttt aaagcgatta
    17881 agtcgctgat tactgacgtg agcgaaacgc ataagatcag cgccgaattg ctggcatcgc
    17941 gtcggcaaat caaccaactg ctgaactggc actggaaact gaaaccgcag aacaatttgc
    18001 cggagctgat ttccggctgg cgtggtgagc tgatggcgga agcattacac aatttattgc
    18061 aggaatatcc gcagtaaaat cttccgaagc cggactgggc gcgctcagcg ccacatccgg
    18121 ctttggcaaa ctacaaatcc aacaccaggc gtttaccctt cgcacgcgaa caacagatca
    18181 acatactttg ctggctggca cgctcttcat cgctaaaata ctgatcccga tggtcagctt
    18241 caccttccag tattgccgtt tcgcaggttc cgcatacccc ttcacgacat aaacattcca
    18301 ctttcgcggc tttattattt tcaataacct gcaaaatagt catctcttcc ggcaccacaa
    18361 actcttttcc ggaacgggca agcaccaggg taaatgcatc gccggttttg tcttcgatag
    18421 caaattgctc aaagtgcagt gtatcggcgg cgatgtccag acgcgcagct tcacttctta
    18481 ccgcttcaat tagcgcctcg gggccacagg tataaacgtg tgtgccaggt tcgatatccg
    18541 ccaataatcg cgctaattcc agtcgtgttc cggttgatga atgatgcaaa tggactttct
    18601 cagcctgcgg atgctggact agctcagcac gaaatgcgca actttctgga tttcgcgagc
    18661 agtaatgtag ctgccagtcg atgtcgctgt gttgcaactc tgccatgtgc gacaggaaag
    18721 gggtgatacc aataccgccc acgataaaca gatgctttct ggctgaggga attagcgcaa
    18781 acaggttatt aggcgttgag atcgttaacc gatcgcccac ttttacctgc tgatgcaaaa
    18841 agcgggaacc gccgcgcgag ttttcttcca gtcgaacggc aatctgataa ctagaggtgt
    18901 tatgcggtga actcagtagt gaatacgcat tgctgtactg gttatcacca tcgctcatct
    18961 gcactaggat gtgacttcct ccggtgaacg caggcagcgg tttgccatcg gttgccacca
    19021 gcgtgaagcg tttcacctgt tcggtaaggg gttcaacctg gctcacctgt acttcaaaca
    19081 tttgatagtc tgacattgct gtactccatc aaaaatcagc ccagcggcgg aaaataccgc
    19141 cgccgatgtc attagtcctt aaacacctgc gccagcagat tatggaaatg ggcgatacca
    19201 tgttcggaaa tgccactacc gctactgtcg gccatgatgc gcccctgacc acgatagcca
    19261 cgcgatttca gccctttctg tacgctttca accagacgta aatcttccgg atggaacaca
    19321 tcgcgatacc actcaatcag cgatttttgc tcgtcggtta actcttcatt ggtgaagtaa
    19381 atatcgtagt tttgcagggt agtttcagaa tccaccggga attcataaat gaccgtcatc
    19441 atccctttga tcggggtgac gttcagcatc gtgcacggcc acagccagaa accgtggaat
    19501 gccgcatccg taccctcttc aaatttaaac gactgttcgg acggtttggc gaaaccgtat
    19561 tgcagcgtcc agttaccgtg catggtgtgc cagtaacgat caacctgtac ggagtcggag
    19621 aaacctagat gcgccggacc acagtgatag cactcgagat agttatcgac aatgttcttc
    19681 cagttggcag gcgtgcgggt ggtaaagcgg gccgctagtt tcagattgtg gacttccggg
    19741 caggcttcca gcactttcgc gcccagaccg gtgtattgat tcacttgaag tacgaaaaaa
    19801 aaccgggagg acattggatt attcgggttc tgatgggatt agatttggtg gggcttgcaa
    19861 gcctgtagcg caaattttag tcgttaatca atgaaacgcg aaagatagta aaaaattgct
    19921 tttgtttcat tgaaaatacg aaaaacaaaa acactgcaaa tcatttcaat aacagcttca
    19981 aaaaacgttc aaaaccgata acaaccaggc tgtaaccaaa tgactcatat cacaaatcag
    20041 ctcatgccgt ttaggtatgt tacatgtgtg attatgtgag gtgaagtatg ttttagctgg
    20101 ttcatggttg ttatacggct ttttttacat cttgtggttc ttgtgaagga gctacaagac
    20161 cttcctgttc ataaatacca tactttgaca aaatcttttt acgagtatct gttggtaatg
    20221 attcaagcat tttggctgtt tgtaaggttt gctgcgaaat accttgcaaa tcagcctgga
    20281 tggaatcatc ggaagaaaaa atctcaggca taagagcttc aaatcctttc tttctgaaca
    20341 actcaatcgc agaatgcagt tccttgtcct ctaacaattc gacaattgcg atcagtttag
    20401 ttttcaatgt gctgcggtca ggttgagaat ttttattctc ttgattattt ttatgctcaa
    20461 ccattggcat gctctgactt tggataaacc actctattgg acgccctgtt gcgtaggcta
    20521 tttgtgctaa tcgatctaaa gacgggtatg tcttgccaga caggtagttt ctgataaccc
    20581 catcagaaag tccgcactta gctgcaaacg cccg
//
LOCUS       DAJTRC010000071        20247 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.071, whole genome shotgun sequence.
ACCESSION   DAJTRC010000071 DAJTRC010000000
VERSION     DAJTRC010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20247
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.071"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            6..320
                     /gene="raiA"
                     /locus_tag="OO357_002745"
     CDS             6..320
                     /gene="raiA"
                     /locus_tag="OO357_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HCR5887637.1"
                     /translation="MEITPAIRQHVADRLAKLEKWQTHLINPHIILSKEPQGFVADAT
                     INTPNGVLVASGKHEDMYTAINELINKLERQLNKLQHKGEARRAATSVKDANFVEEVE
                     EE"
     misc_feature    423..548
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01859"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Phe leader region; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01859"
     gene            424..471
                     /gene="pheL"
                     /locus_tag="OO357_002746"
     CDS             424..471
                     /gene="pheL"
                     /locus_tag="OO357_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phe operon leader peptide"
                     /protein_id="HCR5887638.1"
                     /translation="MKHIPFFFAFFFTFP"
     gene            570..1730
                     /gene="pheA"
                     /locus_tag="OO357_002747"
     CDS             570..1730
                     /gene="pheA"
                     /locus_tag="OO357_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydratase"
                     /protein_id="HCR5887639.1"
                     /translation="MTSENPLLALREKISALDEKLLALLAERRELAVEVGKAKLLSHR
                     PVRDIDRERDLLERLITLGKAHHLDAHYITRLFQLIIEDSVLTQQALLQQHLNKINPH
                     SARIAFLGPKGSYSHLAARQYAARHFEQFIESGCAKFADIFNQVETGQADYAVVPIEN
                     TSSGAINDVYDLLQHTSLSIVGEMTLTIDHCLLVSGTTDLSTINTVYSHPQPFQQCSK
                     FLNRYPHWKIEYTESTSAAMEKVAQAKSPHVAALGSEAGGTLYGLQVLERIEANQRQN
                     FTRFVVLARKAINVSDQVPAKTTLLMATGQQAGALVEALLVLRNHNLIMTRLESRPIH
                     GNPWEEMFYLDIQANLESAEMQKALKELGEITRSMKVLGCYPSENVVPVDPT"
     gene            complement(1773..2894)
                     /gene="tyrA"
                     /locus_tag="OO357_002748"
     CDS             complement(1773..2894)
                     /gene="tyrA"
                     /locus_tag="OO357_002748"
                     /EC_number="1.3.1.12"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional chorismate mutase/prephenate
                     dehydrogenase"
                     /protein_id="HCR5887640.1"
                     /translation="MVAELTALRDQIDEVDKALLNLLAKRLELVAEVGKVKSRFGLPI
                     YVPEREASMLASRRAEAEALGVPPDLIEDVLRRVMRESYSSENDKGFKTLCPSLRPVV
                     IVGGGGQMGRLFEKMLTLSGYQVRILEQHDWDRAADIVADAGMVIVSVPIHVTEQVIG
                     KLPPLPKDCILVDLASVKNGPLQAMLAAHDGPVLGLHPMFGPDSGSLAKQVVVWCDGR
                     KPEAYQWFLEQIQVWGARLHRISAVEHDQNMAFIQALRHFATFAYGLHLAEENVQLEQ
                     LLALSSPIYRLELAMVGRLFAQDPQLYADIIMSSERNLALIKRYYKRFGEAIELLEQG
                     DKQAFIDSFRKVEHWFGDYAQRFQSESRVLLRQANDNRQ"
     gene            complement(2905..3975)
                     /gene="aroF"
                     /locus_tag="OO357_002749"
     CDS             complement(2905..3975)
                     /gene="aroF"
                     /locus_tag="OO357_002749"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase AroF"
                     /protein_id="HCR5887641.1"
                     /translation="MHKDALNNVHITDEQVLMTPEQLKAAFPLSLQQEAQIADSRKTI
                     SDIIAGRDPRLLVVCGPCSIHDPETALEYARRFKALAAEVSDSLYLVMRVYFEKPRTT
                     VGWKGLINDPHMDGSFDVEAGLQIARKLLLELVNMGLPLATEALDPNSPQYLGDLFSW
                     SAIGARTTESQTHREMASGLSMPVGFKNGTDGSLATAINAMRAAAQPHCFVGINQAGQ
                     VALLQTQGNPDGHVILRGGKAPNYSPADVAQCEKEMEQAGLRPSLMVDCSHGNSNKDY
                     RRQPAVAESVVAQIKDGNRSIIGLMIESNIHEGNQSSEQPRSEMKYGVSVTDACISWE
                     MTDALLREIHQDLNGQLTARVA"
     gene            4185..4550
                     /locus_tag="OO357_002750"
     CDS             4185..4550
                     /locus_tag="OO357_002750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2799 domain-containing protein"
                     /protein_id="HCR5887642.1"
                     /translation="MMKKFIAPLLALLVSGCQIDPYTHAPTLTSTDWYDVGMEDAISG
                     SAIKDDDAFSDSQADRGLYLKGYAEGQKKTCQTDFTYARGLSGKSFPASCNNVENASQ
                     LHEVWQKRADENASTIRLN"
     gene            4700..5218
                     /locus_tag="OO357_002751"
     CDS             4700..5218
                     /locus_tag="OO357_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfiR family protein"
                     /protein_id="HCR5887643.1"
                     /translation="MRFSHRLFLLLILLLTGAPILAQEPSDVAKNVRMMVSGIVSYTR
                     WPALSGPPKLCIFSSSRFSTALQENAATSLPYLPVIIHTQQEAMISGCNVFYFGNESP
                     TFQMELTEQYPSKALLLIAEQNTECIIGSAFCLIIHNNDVRFAVNLDASSRSGVKVNP
                     DVLMLARKKNDG"
     gene            5208..6433
                     /gene="dgcN"
                     /locus_tag="OO357_002752"
                     /pseudo
     CDS             5208..6433
                     /gene="dgcN"
                     /locus_tag="OO357_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417095.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcN"
     gene            6449..6931
                     /locus_tag="OO357_002753"
     CDS             6449..6931
                     /locus_tag="OO357_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HCR5887644.1"
                     /translation="MIKHLVAPLIFTSLILTGCQSPQGKFTPEQVAAMQSYGFTESAG
                     DWSLGLSDAILFAKNDYKLLPESQQQIQTMAAKLASTGLTHARMDGHTDNYGEDSYNE
                     GLSLKRANVVADAWAIGGQIPRSNLTTQGLGKKYPIASNKTAQGRAENRRVAVVIATP
                     "
     gene            complement(7008..7355)
                     /gene="rplS"
                     /locus_tag="OO357_002754"
     CDS             complement(7008..7355)
                     /gene="rplS"
                     /locus_tag="OO357_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012768745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HCR5887645.1"
                     /translation="MSNIIKQLEQEQMKQDVPSFRPGDTVEVKVWVVEGSKKRLQAFE
                     GVVIAIRNRGLHSAFTVRKISNGEGVERVFQTHSPVVDSISVKRRGAVRKAKLYYLRE
                     RTGKAARIKERLN"
     gene            complement(7397..8164)
                     /gene="trmD"
                     /locus_tag="OO357_002755"
     CDS             complement(7397..8164)
                     /gene="trmD"
                     /locus_tag="OO357_002755"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HCR5887646.1"
                     /translation="MWIGIISLFPEMFRAITDYGVTGRAVKNGLLSIQSWSPRDFTHD
                     RHRTVDDRPYGGGPGMLMMVQPLRDAIHAAKAAAGEGAKVIYLSPQGRKLDQAGVSEL
                     ATNQKLILVCGRYEGIDERVIQTEIDEEWSIGDYVLSGGELPAMTLIDSVSRFIPGVL
                     GHEASATEDSFAEGLLDCPHYTRPEVLEGMEVPPVLLSGNHAEIRRWRLKQSLGRTWL
                     RRPELLENLALTEEQARLLAEFKTEHAQQQHKHDGMA"
     gene            complement(8195..8743)
                     /gene="rimM"
                     /locus_tag="OO357_002756"
     CDS             complement(8195..8743)
                     /gene="rimM"
                     /locus_tag="OO357_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000043333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HCR5887647.1"
                     /translation="MSKQLTAQAPVDPIVLGKMGSSYGIRGWLRVFSSTEDAESIFDY
                     QPWFIQKAGQWQQVQLESWKHHNQDMIIKLKGVDDRDAANLLTNCEIVVDSSQLPQLE
                     EGDYYWKDLMGCQVVTTEGYDLGKVVDMMETGSNDVLVIKANLKDAFGIKERLVPFLD
                     GQVIKKVDLTTRSIEVDWDPGF"
     gene            complement(8762..9010)
                     /gene="rpsP"
                     /locus_tag="OO357_002757"
     CDS             complement(8762..9010)
                     /gene="rpsP"
                     /locus_tag="OO357_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000256453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HCR5887648.1"
                     /translation="MVTIRLARHGAKKRPFYQVVVADSRNARNGRFIERVGFFNPIAS
                     EKEEGTRLDLDRIAHWVGQGATISDRVAALIKEVNKAA"
     gene            complement(9147..10508)
                     /gene="ffh"
                     /locus_tag="OO357_002758"
     CDS             complement(9147..10508)
                     /gene="ffh"
                     /locus_tag="OO357_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HCR5887649.1"
                     /translation="MFDNLTDRLSRTLRNISGRGRLTEDNVKDTLREVRMALLEADVA
                     LPVVREFINRVKEKAVGHEVNKSLTPGQEFVKIVRNELVAAMGEENQTLNLAAQPPAV
                     VLMAGLQGAGKTTSVGKLGNFLREKHKKKVLVVSADVYRPAAIKQLETLAEQVGVDFF
                     PSDVGQKPVDIVNAALKEAKLKFYDVLLVDTAGRLHVDEAMMDEIKQVHASINPVETL
                     FVVDAMTGQDAANTAKAFNEALPLTGVVLTKVDGDARGGAALSIRHITGKPIKFLGVG
                     EKTEALEPFHPDRIASRILGMGDVLSLIEDIESKVDRAQAEKLASKLKKGDGFDLNDF
                     LEQLRQMKNMGGMASLMGKLPGMGQIPDNVKSQMDDKVLVRMEAIINSMTMKERAKPE
                     IIKGSRKRRIAAGCGMQVQDVNRLLKQFDDMQRMMKKMKKGGMAKMMRSMKGMMPPGF
                     PGR"
     gene            10675..11466
                     /locus_tag="OO357_002759"
     CDS             10675..11466
                     /locus_tag="OO357_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YpjD"
                     /protein_id="HCR5887650.1"
                     /translation="MPVFALLALVAYSVSLALIVPGLLQKNGGWRRMAIISAVIALVC
                     HAIALEARILPDGDSGQNLSLLNVGSLVSLVICTVMTIVASRNRGWLLLPIVYAFALI
                     NLALATFMPNEYITHLEATPGMLMHIGLSLFSYATLIIAALYALQLAWIDYQLKNKKL
                     AFNQEMPPLMSIERKMFHITQIGVVLLTLTLCTGLFYMHNLFSMENIDKAVLSIVAWF
                     VYIVLLWGHYHEGWRGRRVVWFNVAGAVILTLAYFGSRIVQQLIS"
     gene            11487..12773
                     /locus_tag="OO357_002760"
     CDS             11487..12773
                     /locus_tag="OO357_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026171.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HCR5887651.1"
                     /translation="MEHISTTTLIIILIIMVVISAYFSGSETGMMTLNRYRLRHMAKQ
                     GNRSAKRVEKLLRKPDRLISLVLIGNNLVNILASALGTIVGMRLYGDAGVAIATGVLT
                     FVVLVFAEVLPKTIAALYPEKVAYPSSFLLAPLQILMMPLVWLLNAITRMLMRMMGIK
                     TDIVVSGSLSKEELRTIVHESRSQISRRNQDMLLSVLDLEKMTVDDIMVPRSEIIGID
                     INDDWKSILRQLSHSPHGRIVLYRDSLDDAISMLRVREAWRLMSEKKEFTKETMLRAA
                     DEIYFVPEGTPLSTQLVKFQRNKKKVGLVVNEYGDIQGLVTVEDILEEIVGDFTTSMS
                     PTLAEEVTPQNDGSVIIDGTANVREINKAFNWHLPEDDARTVNGVILEALEEIPVAGT
                     RVRIGKYDIDILDVQDNMIKQVKVFPVKPLRESVAE"
     gene            complement(12828..13421)
                     /gene="grpE"
                     /locus_tag="OO357_002761"
     CDS             complement(12828..13421)
                     /gene="grpE"
                     /locus_tag="OO357_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HCR5887652.1"
                     /translation="MSSKEQKTPEGQAPEEIIMDQHEEIEAVEPEASAEQVDPRDEKI
                     ANLEAQLAEAQTRERDGILRVKAEMENLRRRTELDIEKAHKFALEKFINELLPVIDSL
                     DRALEVADKANPDMSAMVEGIELTLKSMLDVVRKFGVEVIAETNVPLDPNVHQAIAMV
                     ESDDVAPGNVLGIMQKGYTLNGRTIRAAMVTVAKAKA"
     gene            13544..14422
                     /gene="nadK"
                     /locus_tag="OO357_002762"
     CDS             13544..14422
                     /gene="nadK"
                     /locus_tag="OO357_002762"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) kinase"
                     /protein_id="HCR5887653.1"
                     /translation="MNNHFKCIGIVGHPRHPTALTTHEMLYRWLCTKGYEVIVEQQIA
                     HELQLKNVKTGTLAEIGQLADLAVVVGGDGNMLGAARTLARYDIKVIGINRGNLGFLT
                     DLDPDNAQQQLADVLEGHYISEKRFLLEAQVCQQDCQKRISTAINEVVLHPGKVAHMI
                     EFEVYIDEIFAFSQRSDGLIISTPTGSTAYSLSAGGPILTPSLDAITLVPMFPHTLSA
                     RPLVINSSSTIRLRFSHRRNDLEISCDSQIALPIQEGEDVLIRRCDYHLNLIHPKDYS
                     YFNTLSTKLGWSKKLF"
     gene            14508..16169
                     /gene="recN"
                     /locus_tag="OO357_002763"
     CDS             14508..16169
                     /gene="recN"
                     /locus_tag="OO357_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HCR5887654.1"
                     /translation="MLAQLTISNFAIVRELEIDFHSGMTVITGETGAGKSIAIDALGL
                     CLGGRAEADMVRTGAARADLCARFSLKDTPAALRWLEENQLEDGHECLLRRVISSDGR
                     SRGFINGTAVPLSQLRELGQLLIQIHGQHAHQLLTKPEHQKFLLDGYANETSLLQEMT
                     ARYQLWHQSCRDLAHHQQLSQERAARAELLQYQLKELNEFNPQPGEFEQIDEEYKRLA
                     NSGQLLTTSQNALALMADGEDANLQSQLYTAKQLVSELIGMDSKLSGVLDMLEEATIQ
                     IAEASDELRHYCDRLDLDPNRLFELEQRISKQISLARKHHVSPEALPQYYQSLLEEQQ
                     QLDDQADSQETLALAVTKHHQQALETARALHQQRQQYAEELAQLITDSMHALSMPHGQ
                     FTIDVKYDEHHLGADGADRIEFRVTTNPGQPMQPIAKVASGGELSRIALAIQVITARK
                     METPALIFDEVDVGISGPTAAVVGKLLRQLGESTQVMCVTHLPQVAGCGHQHYFVSKE
                     TDGAMTETHMQSLDKKARLQELARLLGGSEVTRNTLANAKELLAA"
     gene            16318..16659
                     /gene="bamE"
                     /locus_tag="OO357_002764"
     CDS             16318..16659
                     /gene="bamE"
                     /locus_tag="OO357_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417107.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HCR5887655.1"
                     /translation="MRCKTLTAAAAVLLMLTAGCSTLERVVYRPDINQGNYLTANDVS
                     KIRVGMTQQQVAYALGTPLMSDPFGTNTWFYVFRQQPGHEGVTQQTLTLTFNSSGVLT
                     NIDNKPALSGN"
     gene            complement(16721..17011)
                     /locus_tag="OO357_002765"
     CDS             complement(16721..17011)
                     /locus_tag="OO357_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RnfH family protein"
                     /protein_id="HCR5887656.1"
                     /translation="MLGKIAVEVAYALPEKQYLQRVTLQEGATVEEAIRASGLLELRT
                     DIDLTKNKVGIYSRPAKLSDSVHDGDRVEIYRPLIADPKELRRQRAEKSANK"
     gene            complement(17001..17438)
                     /gene="ratA"
                     /locus_tag="OO357_002766"
     CDS             complement(17001..17438)
                     /gene="ratA"
                     /locus_tag="OO357_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010376629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin RatA"
                     /protein_id="HCR5887657.1"
                     /translation="MPQISRTALVPYSAEQMYQLVNDVQSYPQFLPGCTGSRILESTP
                     GQMTAAVDVSKAGISKTFTTRNQLTSNQSILMNLVDGPFKKLIGGWKFTPLSQEACRI
                     EFHLDFEFTNKLIELAFGRVFKELAANMVQAFTVRAKEVYSAR"
     gene            17609..18091
                     /gene="smpB"
                     /locus_tag="OO357_002767"
     CDS             17609..18091
                     /gene="smpB"
                     /locus_tag="OO357_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HCR5887658.1"
                     /translation="MTKKKAHKPGSATIALNKRARHEYFIEEEFEAGLALQGWEVKSL
                     RAGKANISDSYVLLRDGEAFLFGANITPMAVASTHVVCDPTRTRKLLLNQRELDSLYG
                     RVNREGYTVVALSLYWKNAWCKVKIGVAKGKKQHDKRSDIKEREWQVDKARIMKNAHR
                     "
     gene            18306..18668
                     /gene="ssrA"
                     /locus_tag="OO357_002768"
     tmRNA           18306..18668
                     /gene="ssrA"
                     /locus_tag="OO357_002768"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            18873..20114
                     /locus_tag="OO357_002769"
     CDS             18873..20114
                     /locus_tag="OO357_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5887659.1"
                     /translation="MARQTKPLSVKEIESAKPKEADYVLYDGDGLELLIKSSGSKIWQ
                     FRYIRPVTKTRAKKSIGPYPSVTLADARNYRAESRSLLAKQIDPQEHQQEQLRSSLEA
                     KTNTFQLVAERWWNVKKASVTEDYAEDIWRSLERDVFPAIGDVSVTDIKAHTLVQAVQ
                     PVQARGALETVRRLCQRINEVMIYAQNTGLIDAVPSVNIGKAFEKPQKKNMPSIRPDQ
                     LSQLMQTMRTASISLSTRCLFMWQLLTITRPAEAAEARWEEVDIEAQEWKIPAARMKM
                     NRDHTVPLSDEAIAVLEMMKPLSGNREFIFPSRIKPNQPMNSQTVNASLKRAGFGGVL
                     VSHGLRSIASTALNEQGFPPDAIEAALAHVDKNEVRRAYNRSDYLEQRRPMMQWWANF
                     VMAADRGSMIEGGIKGMKLVG"
BASE COUNT         5207 a         5304 c         4766 g         4970 t
ORIGIN      
        1 aacaaatgga aattactccg gccatccgcc aacatgtcgc agaccgtctc gccaaactgg
       61 aaaaatggca aacacatctg attaatccac atatcattct gtccaaagag ccacaagggt
      121 ttgttgctga cgccacaatc aatacaccta acggcgttct ggttgccagt ggtaaacatg
      181 aagatatgta caccgcaatt aacgaattga tcaacaagct ggaacggcag ctcaataaac
      241 tgcagcacaa aggcgaagca cgtcgtgccg caacatcggt gaaagacgcc aacttcgtcg
      301 aagaagttga agaagagtag tcctttatat tgagtgtatc gccaacgcgc cttcgggcgc
      361 gttttttgtt gacagcgtga aaacagtacg ggtactgtac taaagtcact taaggaaaca
      421 aacatgaaac acataccgtt tttcttcgca ttctttttta ccttcccctg aatgggaggc
      481 gtttcgtcgt gtgaaacaga atgcgaagac gaacaataag gcctcccaaa tcggggggcc
      541 ttttttattg ataacaaaaa ggcaacacta tgacatcgga aaacccgtta ctggcgctgc
      601 gagagaaaat cagcgcgctg gatgaaaaat tattagcgtt actggcagaa cggcgcgaac
      661 tggccgtcga ggtgggaaaa gccaaactgc tctcgcatcg cccggtacgt gatattgatc
      721 gtgaacgcga tttgctggaa agattaatta cgctcggtaa agcgcaccat ctggacgccc
      781 attacattac tcgcctgttc cagctcatca ttgaagattc cgtattaact cagcaggctt
      841 tgctccaaca acatctcaat aaaattaatc cgcactcagc acgcatcgct tttctcggcc
      901 ccaaaggttc ttattcccat cttgcggcgc gccagtatgc tgcccgtcac tttgagcaat
      961 tcattgaaag tggctgcgcc aaatttgccg atatttttaa tcaggtggaa accggccagg
     1021 ccgactatgc cgtcgtaccg attgaaaata ccagctccgg tgccataaac gacgtttacg
     1081 atctgctgca acataccagc ttgtcgattg ttggcgagat gacgttaact atcgaccatt
     1141 gtttgttggt ctccggcact actgatttat ccaccatcaa tacggtctac agccatccgc
     1201 agccattcca gcaatgcagc aaattcctta atcgttatcc gcactggaag attgaatata
     1261 ccgaaagtac gtctgcggca atggaaaagg ttgcacaggc aaaatcaccg catgttgctg
     1321 cgttgggaag cgaagctggc ggcactttgt acggtttgca ggtactggag cgtattgaag
     1381 caaatcagcg acaaaacttc acccgatttg tggtgttggc gcgtaaagcc attaacgtgt
     1441 ctgatcaggt tccggcgaaa accacgttgt taatggcgac cgggcaacaa gccggtgcgt
     1501 tggttgaagc gttgctggta ctgcgcaacc acaatctgat tatgacccgt ctggaatcac
     1561 gcccgattca cggtaatcca tgggaagaga tgttctatct ggatattcag gctaatcttg
     1621 aatcagcgga aatgcaaaaa gcattgaaag agttagggga aatcacccgt tcaatgaagg
     1681 tattgggctg ttacccaagt gagaacgtag tgcctgttga tccaacctga tgaaaaggtg
     1741 ccggatgatg tgaatcatcc ggcactggat tattactggc gattgtcatt cgcctgacgc
     1801 aataacacgc ggctttcact ctgaaaacgc tgtgcgtaat cgccgaacca gtgctccacc
     1861 ttgcggaaac tgtcaataaa cgcctgctta tcgccctgct ccagcaactc aatcgcctcg
     1921 ccgaaacgct tatagtaacg tttgattaac gccagattac gctctgacga cataatgatg
     1981 tcggcataaa gctgcggatc ctgagcaaac agtcgcccga ccatcgccag ctcaaggcgg
     2041 taaatcggcg aagagagcgc cagaagttgc tcaagctgaa cattttcttc tgccaggtgc
     2101 agcccgtaag caaaagtagc aaagtggcgc agagcctgaa taaacgccat attctgatcg
     2161 tgctcgacag cgctaatacg atgcaaccga gcgccccaga cctgaatttg ctccagaaac
     2221 cattggtatg cttccggctt acgtccatca caccagacca caacttgctt tgccaggcta
     2281 ccgctgtccg ggccgaacat cgggtgtaac cccagtaccg ggccatcgtg cgccgccagc
     2341 atggcctgta atggtccatt tttcactgat gccagatcaa ccagaataca atctttcggt
     2401 aaaggcggta atttgccgat aacttgctca gtaacgtgga ttggcacact aacaatcacc
     2461 attccggcat cggcaacaat atcagccgct cgatcccagt catgttgctc cagaatccgc
     2521 acctgataac ccgatagtgt cagcatcttc tcgaacaggc gtcccatctg accgccaccg
     2581 ccgacgataa ccaccggacg cagtgacgga caaagtgttt taaatccttt gtcgttttca
     2641 ctggagtaag attcacgcat cacccgacgc aaaacatcct caatcagatc tggcggtaca
     2701 cccagagctt ccgcctctgc acgacgcgag gccaacatag atgcctcgcg ctccggaaca
     2761 taaataggca gtccaaagcg gcttttcacc ttgcccactt cagcaaccag ttccagacgc
     2821 ttcgctaata aattcagcag cgctttatcg acttcatcaa tttgatcgcg taatgcggtc
     2881 aattcagcaa ccataataaa cctcttaagc cacgcgagcc gtcagctgcc cgttcagatc
     2941 ctgatgaatt tcacgcagca aggcatcggt catttcccag ctaatgcagg catcggttac
     3001 ggatacaccg tatttcattt cactgcgcgg ttgctcagaa gactgattgc cctcgtggat
     3061 attactttcg atcatcagac caataattga gcgattacca tctttgattt gagcaaccac
     3121 ggattctgcc accgcaggct gacggcgata atctttattg gaattaccgt ggctgcaatc
     3181 taccatcaga gacgggcgca gtcccgcctg ttccatctct ttttcacatt gcgcaacatc
     3241 cgcagggcta tagttcggcg ctttaccacc gcgcaggatc acatgcccgt ccggattccc
     3301 ctgagtttgt agcaacgcaa cctgccctgc ctggttaatg ccaacaaaac agtgcggctg
     3361 ggcggcggcg cgcatagcgt taattgctgt tgccagactg ccgtcggtgc cgtttttaaa
     3421 accaaccggc atggaaagcc cggaggccat ttcacggtga gtttgcgatt ccgttgtacg
     3481 agcaccaatt gctgaccagc taaacagatc gcccaggtat tgcgggctat tcggatctaa
     3541 cgcttccgtc gccagtggca gtcccatatt caccagctca agcaacaatt tacgcgcgat
     3601 ttgcagcccg gcttctacat caaaagagcc atccatatgg ggatcgttaa ttaacccttt
     3661 ccagccgaca gtggtacggg gtttttcaaa atagacgcgc attaccagat agaggctatc
     3721 gctgacctct gcggcaagag ctttaaatcg acgagcatat tccagagcag tttccggatc
     3781 atgaatggaa caaggaccac atactaccag cagacgagga tcgcgcccgg cgataatatc
     3841 tgaaatggtt ttacgcgagt cagcaatctg ggcttcttgt tgcaggctca atggaaaagc
     3901 ggccttcagt tgttccggag tcattaaaac ctgttcgtcg gtaatatgta cgttattcag
     3961 cgcgtcttta tgcatgatgg cgatcctgtt tatgctcgtt tgcgatagtt gctcctcagc
     4021 gaggatgacg taacgataac acataaagta aagttttcaa tccatatttc gtacattttt
     4081 atttacacag gcaatttagt cgcgctttca acccttgctt ctgtatagat aaatttacat
     4141 cccctttaaa aacaatccgc tatgctttga aaaaggagaa agaaatgatg aaaaagttta
     4201 tcgccccctt gttggcttta ctggttagcg gatgtcagat tgatccttat actcacgcgc
     4261 caaccttgac cagcaccgac tggtatgatg tcggtatgga agatgcgata tcgggcagcg
     4321 ccataaaaga tgacgatgca tttagcgatt cacaggcgga tcgcggtcta taccttaaag
     4381 gatatgccga agggcaaaag aaaacttgcc agaccgattt tacttatgcc cggggacttt
     4441 ccggtaaaag ctttcctgcc agctgcaaca atgttgaaaa tgccagccaa ctgcatgaag
     4501 tatggcaaaa aagggctgat gaaaatgcca gcacaatacg attaaattaa ttaaaacccc
     4561 acaaataaat taaacataag attttactca ttgtgtttta tttctcacat tgatgacggt
     4621 cgcatgaaaa aatgatattt taatgagcat cctgaaaaaa tatcgatgca tttcgagcga
     4681 agatggtggg gatccctgaa tgcgtttttc tcaccgactg ttcttacttc tgattctcct
     4741 gctgacggga gcaccaatac tcgctcaaga gccttctgac gttgcgaaaa acgtgcgtat
     4801 gatggtttcc ggtattgtca gttacacccg ctggcctgcg ctatcagggc caccaaagtt
     4861 atgtatcttt tcatcttcac gttttagtac cgccctacaa gagaatgccg cgacatcttt
     4921 gccttatctg cctgtcatca ttcacactca acaagaggcg atgatttcag gctgcaatgt
     4981 tttttatttt ggcaacgagt caccgacatt ccagatggaa ttaaccgaac aatatccgtc
     5041 aaaggcgtta ttattaattg ccgaacaaaa taccgagtgc attattggta gcgccttttg
     5101 tctgatcatc cacaataatg acgtcagatt tgccgtaaac ctggatgcct catcgcgtag
     5161 cggtgtaaag gtcaacccgg atgtcttaat gctcgcacgg aagaaaaatg atggataacg
     5221 ataattctct taataagcgc cccacgttta aaagagcatt acgcaacatc agtatgacca
     5281 gcatatttat cactatgatg ctgatctggt tgctgctttc cgtgacctcg gtgctgaccc
     5341 tgaaacagta cgcgcaaaaa aacctggcac tgacagcagc aacaatgact tacagtctgg
     5401 aagcagctgt cgtttttgcc gatggccctg cagcaactga aacactggca gcgctgggcc
     5461 agcaagggca ttttcaactg cagaagtacg tgataagcag caaaatattc tggcatcctg
     5521 gcattacccc cgtaaggatc caggcgatac tttcagcaat ttcataagcc actggctctt
     5581 ccctgccccc atcattcagc cgattcgtca caatggtgaa accattggcg aagtacgctt
     5641 aaccgctcgc gacagttcaa tcagccattt tatctggttt tcgctcgccg tactgaccgg
     5701 ttgtattctg ctggcatcag gcatcgcaat taccctcacc cgccatttgc acaatggcct
     5761 ggtggaagca ctgaaaaata tcaccgatgt cgtacatgat gtgcgttcca accgcaattt
     5821 ttcccgacga gtttcggaag aacgtatcgc tgagtttcac cgcttcgctc tcgacttcaa
     5881 cagtctgctg gatgaaatgg aagagtggca gcttcgttta caggccaaaa atgcgcagct
     5941 tctgcgtacc gcgctacatg acccattaac cgggctggct aaccgcgcag cgtttcgtag
     6001 cggcatcaat acgttgatga acaattccga tgcccgaaaa acgtcggcgt tactatttct
     6061 tgatggcgat aatttcaaat atatcaatga tacctggggt catgcgacgg gcgatagggt
     6121 cttgattgaa atcgcaaaac ggttagctga atttggcggg ctgcgacata aagcataccg
     6181 cctgggcggc gatgaattcg ctatggtgct ctatgatgta cagtcggaat ctgaagtgca
     6241 gcagatatgc tcagcactga cacaaatctt taatctcccg tttgatcttc ataatggcca
     6301 tcagaccacc atgacattaa gcattggtta cgcgatgacc attgagcacg cctctgcgga
     6361 aaaattacaa gagcttgccg atcacaatat gtatcaggcc aaacaccagc gtgccgaaaa
     6421 gctggtgaga taacaaggat atatcgatat gataaagcac ctggtagcac ccctgatttt
     6481 cacctcacta atactgactg gctgccagtc ccctcaggga aagtttactc ctgagcaagt
     6541 cgccgctatg caatcttatg gatttactga atccgccggc gactggtcgc tgggcttatc
     6601 agatgccatt ctgttcgcaa aaaatgacta caaattgctc ccggaaagcc agcaacagat
     6661 ccaaaccatg gcagccaaat tggcctcgac agggctaaca catgcccgta tggatggaca
     6721 caccgataac tatggtgaag acagttacaa cgaaggctta tcattgaaac gggcgaatgt
     6781 cgtggccgat gcatgggcta ttggtggaca aattccacgc agcaatctca ccacacaggg
     6841 tttaggaaaa aaatatccca tagccagtaa caagaccgcc cagggccgcg ccgagaaccg
     6901 ccgcgtcgca gtggtgattg caacccctta aaacacgagc aaacagacaa aaaaagggcc
     6961 agccaattgg ccagcccttc ttaacaggat gtcgcttaag cgaaatctta gttaagacgc
     7021 tctttgatac gagcagcctt accagtacgc tcacgcaggt agtacagttt agctttacga
     7081 acagcaccac gacgtttgac agaaatgctg tcaactaccg gagagtgagt ctggaagaca
     7141 cgctcaacgc cttcgccgtt ggaaatttta cgaacagtga atgcagagtg cagaccgcgg
     7201 ttacgaatag cgataaccac gccctcgaat gcctgcagac gttttttgga accttcaaca
     7261 acccatactt tcacttccac ggtatcaccc ggacggaagg aaggtacgtc ctgcttcatc
     7321 tgctcttgtt caagttgctt aataatgttg ctcataattt aatctcttat cctgggtaaa
     7381 ctgatctctc gggggcttac gccatcccat catgtttatg ttgctgttgt gcgtgttccg
     7441 ttttgaactc cgccagcaac cttgcttgct cttcagtcag agccaggttt tccagaagtt
     7501 caggtcttct aagccaggta cggcccagcg actgtttcaa acgccagcga cgtatctcgg
     7561 catggttgcc cgacagtaac actggcggaa cttccatccc ttctaacacc tcaggccgcg
     7621 tatagtgcgg gcaatccagc aatccttcag caaaggaatc ttccgttgcc gaggcttcat
     7681 gtcccagtac tcccggaata aaccgggaaa cggagtcaat cagcgtcatt gctggtaact
     7741 caccaccact gagaacgtaa tcgccgattg accattcttc gtcaatttcg gtttggatca
     7801 cgcgctcatc tataccttcg tagcgaccgc acaccagaat caatttttga ttcgttgcca
     7861 gttcgctgac gcccgcttga tcaagcttgc gtccctgtgg tgacagataa atcacctttg
     7921 cgccttcacc cgccgcggct tttgctgcat gaatggcgtc ccgcaagggt tgcaccatca
     7981 ttaacatccc cggtccgccg ccgtaaggac gatcgtccac ggtacggtgc cggtcatgcg
     8041 tgaagtcgcg aggactccaa ctctggatgc tcagcaggcc atttttaact gcccggccag
     8101 ttaccccgta atcggtaatt gcgcggaaca tttcaggaaa caggctaatt atgccaatcc
     8161 acatagcgcc gccttttacc gtttatccgg tggtttaaaa accaggatcc caatctactt
     8221 cgattgaacg agtagtgaga tcgactttct tgataacctg cccatcgagg aacggtacga
     8281 gacgttcctt gataccaaac gcatctttca ggtttgcctt aatgacgaga acgtcattag
     8341 atccggtttc catcatatca acgactttac cgagatcgta gccttcagtg gttactacct
     8401 ggcagcccat caggtctttc cagtagtagt ccccctcttc aagctgaggc agctgcgatg
     8461 aatccacgac aatttcacaa ttcgtcagca ggttcgccgc atcacgatcg tcaacgcctt
     8521 tcagcttgat gatcatgtcc tgattgtggt gcttccagct ttccagctgg acttgctgcc
     8581 actgacccgc cttctggata aaccagggct gatagtcaaa aatgctttcg gcgtcttcgg
     8641 tggaagaaaa cactctgagc cacccacgaa taccgtaaga cgaacccatt tttcccaaaa
     8701 cgatgggatc aacaggtgct tgcgcggtga gttgtttgct catcatgacc accgtgacag
     8761 attaagctgc tttgtttact tctttgatca gcgcagcaac gcgatcagaa atagttgcgc
     8821 cctggccaac ccagtgagcg atgcgatcca gatccaggcg agtgccttct tctttttcgc
     8881 tagcgattgg gttgaagaaa ccaacgcgct cgatgaagcg accgttgcgt gcattacggc
     8941 tgtcagcgac aacaacctgg tagaacggac gctttttagc gccgtgacgt gctaaacgaa
     9001 tagttaccat aacatcctct tgtgtgaata aaacaaccgg accccatcga ggaacggagt
     9061 ccggtgtcat attaaaagcc cgaaaatttt actgatttct ggagaaaatg caatcagcag
     9121 ttaataactc tgtcgcataa ggtcatttag cgaccaggaa atcccggcgg catcattccc
     9181 ttcatgcttc tcatcatctt cgccattccg cctttcttca ttttcttcat catgcgctgc
     9241 atgtcgtcga actgcttcag aaggcggtta acgtcctgta cctgcatccc gcaaccggcg
     9301 gcgatacgac gcttacgcga acctttgatg atttctggct tagcgcgctc tttcatcgtc
     9361 attgagttga tgatggcttc catacgcacc agcactttat cgtccatctg cgacttgacg
     9421 ttatccggga tctgccccat gcccggcagt ttgcccatca agctggccat gccgcccata
     9481 tttttcatct ggcgcagttg ctcaagaaag tcgttgagat cgaagccgtc gccttttttt
     9541 agcttgctgg ctaatttctc tgcctgcgcg cggtcaactt tgctttcgat atcttcgatc
     9601 agcgacagta cgtcgcccat gccgagaata cgcgacgcga tgcggtccgg atggaacggc
     9661 tccagcgcct cagttttctc gccaacaccg aggaacttga tcggtttgcc agtgatgtga
     9721 cgaatagaga gcgccgcacc gccgcgggca tcgccgtcca ctttggtcaa cactacgccg
     9781 gtaagcggta acgcttcatt gaatgctttt gccgtattgg ccgcatcctg accggtcatg
     9841 gcgtcaacca caaacagggt ttcaaccggg ttaatcgacg catggacttg tttgatctcg
     9901 tccatcatcg cttcgtcaac gtgcagacga ccagcggtat ccaccagcag cacgtcgtag
     9961 aatttcagtt tggcttcttt cagcgccgcg ttaacaatat caaccggctt ctgaccaaca
    10021 tcagaaggga agaaatcaac gcccacctgc tctgccagcg tctcaagctg tttgattgcc
    10081 gccgggcgat agacgtcagc agaaaccacc agcactttct tcttgtgctt ctcgcgcagg
    10141 aaattaccga gtttaccaac gctggttgtt ttaccggcac cttgcaggcc tgccatcagc
    10201 acgaccgcag gcggttgcgc agccagattc agggtctggt tctcttcgcc catcgccgca
    10261 accagttcgt tacggacgat tttgacgaac tcctgccccg gcgtcaggct cttattaact
    10321 tcatgaccaa ccgctttctc ttttacgcga ttgataaact cacgcactac cggcagagcg
    10381 acgtccgcct ccagcagcgc catgcgcact tcgcgcagcg tatcttttac gttgtcttca
    10441 gtgaggcgtc cacggccact gatattgcgc agcgtgcgcg acaaacgatc ggttaaatta
    10501 tcaaacattg tctctcgcct ggggtggaaa cggttggccg caatcgcgac acatcatcag
    10561 tattttgccg cagtataaca tgaaggcgtc tttgttgtta tgcaacggtt ggagcagcgt
    10621 tcacctgacg ctatactgct tctctttctt attgctcaaa ctgtcgacat tactatgccc
    10681 gtttttgctc tgctcgcgct tgtcgcctac tccgtcagtc ttgcgctgat tgttcccggt
    10741 ctgctgcaaa aaaacggcgg ctggcggcgc atggctatta tttctgcggt cattgcgctg
    10801 gtctgccacg caatcgctct ggaagcccgc atcctgcccg acggtgatag cggacaaaac
    10861 ctcagcctgc tgaacgttgg ttcattggtc agtttggtga tctgtacggt aatgaccatt
    10921 gtggcttctc gcaatcgtgg ctggttgctg ctacccattg tctatgcctt tgcgcttatc
    10981 aacctggcgc tggcgacctt catgcccaat gaatacatca cccatctgga agcaacgcct
    11041 gggatgctga tgcacattgg cttatcgctc ttttcctatg ccacgctaat tatcgccgcc
    11101 ctgtacgcgc tgcaactggc gtggattgat taccaactga agaacaagaa gctggcattt
    11161 aaccaagaaa tgccgccatt gatgagtatc gagcgtaaaa tgttccacat cacgcagatt
    11221 ggcgtggtgc tgctaacgct cacgctttgc actggcctgt tctacatgca caacctattt
    11281 agcatggaaa atatcgacaa ggctgtgctc tctatcgtgg cgtggtttgt ctatattgtg
    11341 ctgctgtggg gacattacca tgaaggatgg cgtggacgcc gcgtcgtctg gtttaacgtt
    11401 gcgggcgcgg taattctgac actggcctac ttcggcagcc gaattgtcca gcagttaatc
    11461 agctaaaccc agaaaaggag tttcccctgg aacacatttc tactactacg ttgatcatta
    11521 ttctgatcat catggtggtc atttcagcct atttttccgg gtccgaaacc ggaatgatga
    11581 ccctcaaccg ctatcgtctt cgacatatgg cgaaacaggg taatcgctcg gccaaacgcg
    11641 tcgaaaaatt gctgcgtaag ccagaccgcc tgataagcct ggtgttaatc ggcaataacc
    11701 tggtcaatat tcttgcctcc gcgctcggca ctattgtcgg gatgcgtttg tacggcgatg
    11761 cgggcgtggc aattgcgact ggtgtgctga cttttgtcgt actggttttt gctgaggtat
    11821 tgccgaaaac cattgccgcg ctgtacccgg aaaaagtcgc ttatccgagt agttttctgc
    11881 tggctccgct gcaaattttg atgatgccgc tggtctggtt gctgaatgct atcacccgta
    11941 tgctgatgcg catgatgggt atcaaaaccg atatcgtggt tagcggctct ttgagcaaag
    12001 aagagttgcg cactatcgtg catgaatcgc gctcacaaat ttcccgtcgc aatcaggata
    12061 tgctgctgtc ggtgctcgat ctggaaaaaa tgaccgttga tgacatcatg gtaccgcgca
    12121 gtgaaattat cggtattgat atcaacgatg actggaaatc tattctgcgc caactctccc
    12181 actcacctca cgggcgcatc gtgctttacc gtgattcgct ggacgacgcc atcagtatgc
    12241 tgcgagtacg tgaagcctgg cggttgatgt cggagaaaaa agagttcacc aaagaaacca
    12301 tgctgcgcgc cgcggacgag atctattttg tgcctgaagg tacgccgctc agcacgcagt
    12361 tggtaaagtt tcagcgcaac aaaaagaaag tcggcctggt cgtcaacgag tatggagaca
    12421 ttcaggggct ggtgacggtt gaagatattc tggaagagat tgttggcgat ttcactacgt
    12481 cgatgtcgcc aacacttgcc gaagaggtca cgccgcaaaa cgacggttcg gtgattatcg
    12541 atggcaccgc caacgtgcgg gaaatcaaca aagcctttaa ctggcatcta ccggaagatg
    12601 atgcccgcac ggttaatggc gtcattcttg aggcactgga agagatccct gtcgcaggca
    12661 cccgcgtgcg tattggcaag tacgatatcg atattctcga cgtacaggac aatatgatta
    12721 agcaggtaaa agtttttcct gtgaaaccgc tgcgcgagag tgtggcggag taacgaaaac
    12781 ggccctgcat tcgcatgcag ggccgtgaat tattacgaaa acagaaatta cgcttttgct
    12841 ttcgctacag ttaccatcgc cgcacgaatc gtacgaccat tcagcgtata acccttctgc
    12901 ataatgccca gtacgttacc tggcgcaacg tcatcagatt ccaccattgc gatagcctga
    12961 tgcacattcg ggtccagcgg cacgttagtt tcggcgatca cttcaacacc aaacttacgc
    13021 acaacatcca gcatcgactt cagcgtcagc tcaatgcctt caaccatcgc agacatatcc
    13081 gggttagctt tatcagccac ttccagcgca cgatccaggc tatcaatcac cggcagcaat
    13141 tcgttgatga atttctccag cgcgaatttg tgggcttttt caatatccag ttcagtacga
    13201 cgacgcaggt tttccatttc ggcttttacg cgcaaaatac cgtcacgttc acgggtctgg
    13261 gcttcagcca gctgagcttc gagattcgca attttttcat cgcgcggatc cacctgctca
    13321 gcagaagctt ctggctcaac tgcctcaatc tcttcgtgct gatccatgat aatttcttcc
    13381 ggggcttgcc cctcaggcgt tttctgttct ttactactca tgaatttctc cgcgtttttt
    13441 tcgcattcat ctcgctaact tcgcttatta tggggatcag tttcagggtt tcaagggaag
    13501 cactcacatt gtcatcaatc ttcgcaacaa ggacctcgga aaaatgaata atcatttcaa
    13561 gtgtattggc attgtgggac acccacggca ccctactgca ctgacaacac atgaaatgct
    13621 ctaccgctgg ctgtgcacaa aaggttacga ggtcatcgtt gagcaacaaa tcgctcacga
    13681 actgcaactg aagaatgtga aaactggcac gctcgcggag attgggcaac tggctgatct
    13741 cgcggtagtc gttggtggcg acggtaatat gctgggcgcg gcgcgcacgc tcgcccgtta
    13801 cgatattaaa gttattggaa tcaaccgtgg caacctgggt ttcctgactg accttgaccc
    13861 cgataacgcc cagcaacaat tagccgatgt gctggaaggt cactacatca gcgagaaacg
    13921 ttttttgctg gaagcgcaag tctgtcagca agattgccaa aaacgcatca gcaccgcgat
    13981 taacgaagtg gtacttcatc ctggcaaagt ggcgcatatg atagagttcg aagtgtatat
    14041 cgacgagatc tttgcgtttt ctcagcgatc tgatgggcta attatttcga cgccaacagg
    14101 ctccaccgcc tattccctct ctgcgggcgg tcctattctg accccctctc tggatgcgat
    14161 taccctggtg cccatgttcc cgcatacgtt gtcagcacga ccactggtca taaacagcag
    14221 cagcacgatc cgtctgcgtt tttcgcatcg ccgtaacgac ctggaaatca gttgcgacag
    14281 ccagatagca ctgccgattc aggaaggtga agatgtcctg attcgtcgct gtgattacca
    14341 tctgaatctg attcatccga aagattacag ttatttcaat acattaagca ccaagctcgg
    14401 ctggtcaaaa aaattattct aattttacgc cagcctcttt actgtatata aaaccagttt
    14461 atactgtaca caataacagt aatggttttt catacaggaa aacgactatg ttggcacaac
    14521 tgaccatcag caactttgct atcgttcgtg agcttgagat tgattttcat agcggcatga
    14581 ccgtaataac tggcgagacc ggtgcgggta aatctattgc aatagatgcc ctcggtcttt
    14641 gtctcggtgg tcgcgctgaa gccgacatgg tgcgtaccgg cgctgctcgc gctgacctgt
    14701 gcgcccgttt ttctctgaaa gatacgccag cggccctgcg ctggctggaa gaaaaccagc
    14761 ttgaagacgg gcatgaatgt ttgcttcgtc gcgtgatcag cagtgatggt cgctcccgtg
    14821 gtttcatcaa cggtacagct gttcctctgt cacaactgcg cgaactgggt cagttgctga
    14881 ttcagatcca tggtcagcac gctcatcaat tactcaccaa acctgagcac caaaaattcc
    14941 tgcttgatgg ctatgccaat gaaacctctc tacttcagga aatgaccgca cgttatcagt
    15001 tgtggcatca aagctgccgt gacctcgcgc atcatcaaca gttaagtcag gaacgcgccg
    15061 cccgtgcgga actgctgcaa taccaattaa aagaacttaa cgaatttaat ccgcagcccg
    15121 gagagtttga gcaaatcgac gaagagtaca aacgtctggc gaacagcggt caattgctga
    15181 ccaccagcca gaatgcattg gcattaatgg ccgacggtga agacgcaaac ctgcaaagtc
    15241 agctttacac ggctaaacaa ctggtgagcg aattgattgg catggacagc aaactgtccg
    15301 gcgtacttga tatgctggaa gaagctacca tccagattgc tgaagccagc gacgaactgc
    15361 gccactactg cgatcgtctg gatctcgatc ccaaccgact gtttgaactt gaacagcgca
    15421 tctcaaaaca gatttcgctg gcacgtaaac atcacgtcag ccctgaggca ttaccacagt
    15481 attaccagtc gctactggaa gaacagcagc aactggacga tcaggccgac tcacaagaaa
    15541 cgctcgcgct ggcggtaacg aaacatcatc agcaggcact ggaaaccgcg cgcgcattac
    15601 accaacaacg ccagcaatat gcagaagaac ttgcacagct gatcaccgac agtatgcatg
    15661 cgctctcaat gccgcatggg cagtttacga ttgatgtcaa atatgacgag catcacctgg
    15721 gcgctgacgg tgccgatcgt attgagtttc gggtaaccac caacccaggt cagccaatgc
    15781 agcctattgc caaagtcgca tccggtggtg aattgtcccg catcgcactg gcaatccagg
    15841 tcatcacggc gcgtaaaatg gaaaccccgg cactgatttt tgatgaagtg gatgtaggga
    15901 ttagcggtcc aacagcggca gttgtcggca aactgctgcg tcaactcggc gaatcaactc
    15961 aggtgatgtg tgttacccac ctgccacaag tcgcgggatg tggtcatcaa cactattttg
    16021 tcagcaaaga aaccgatggt gcgatgacag aaacacatat gcaatctctg gataaaaaag
    16081 cgcggttaca agagctggcg cgcctgcttg gtggcagtga agtcacacgt aacacactgg
    16141 cgaatgcgaa agaactgctt gcagcgtaaa cttttttcct gcttcacggt cagagtaaac
    16201 agcaaaacgc cgtaagaccg gaaagcaaaa ggttttaaag tgatgaaagg tctattatca
    16261 tcggcatatt acagatgagc cacgtactgc tcgggcccga aaaggaatca aatcactatg
    16321 cgctgtaaaa cgctgactgc tgcagcagca gtactattga tgttaaccgc aggctgttcc
    16381 actctggagc gagtggttta ccgtcctgac atcaaccagg ggaactatct gaccgctaac
    16441 gacgtatcca aaatacgtgt tggcatgacg caacaacaag ttgcgtacgc attgggtaca
    16501 ccgctgatgt ccgatccatt tggtacgaat acctggttct atgtcttccg ccagcaacca
    16561 ggtcatgaag gtgtaacgca gcaaacgctg acgctgacct ttaacagtag cggtgtgttg
    16621 accaatattg ataacaaacc tgcgctgagt ggtaactaat aataaagttg ctctcaaaga
    16681 cgttaaaaaa ggtgctcaat gagcaccttt tttctgtctg ttatttattc gctgattttt
    16741 ctgctcgttg cctgcgaagc tctttcggat cggcaatgag agggcgataa atctccaccc
    16801 gatcgccatc atgcacacta tcgcttagtt ttgccggacg gctgtaaatg ccgactttgt
    16861 ttttagttaa atcgatatcg gtacgcaatt ccagcaagcc actggcgcga atagcttctt
    16921 caaccgtcgc gccctcctgc agcgtcactc gctgcaggta ttgcttctca ggtagcgcat
    16981 aagccacctc aacggcaatt ttacctagca ctgtaaacct ctttcgcacg aaccgtaaaa
    17041 gcctggacca tattagccgc cagctcttta aacacgcgac caaaggcgag ttcaatcaac
    17101 ttattggtaa actcaaagtc gagatgaaac tcgatacgac acgcctcctg gctcagcggc
    17161 gtaaacttcc atccaccaat caatttcttg aacggcccat ccaccagatt cataagaata
    17221 ctttggttac tggtcaactg gttgcgggta gtaaacgttt tgctgatccc agccttagag
    17281 acatctaccg ccgcagtcat ctgcccagga gtggactcca gaatccgact tccggtacaa
    17341 cctggcaaaa actgaggata agactgaacg tcattcacta actgatacat ttgctccgcg
    17401 ctgtagggta ccagtgcggt ccggctaatc tgaggcataa caatttccat caacaaaaat
    17461 ccaacaaata atatcattta tcctgctgta aaaaaaacgc tatcccggcg ctgggtaaca
    17521 tcgggttcat gctaagatag agccttgtcc cccgcaggat tgatatgggg tattttcgat
    17581 ttcagattac cgatgattca cgacgcttat gacgaagaaa aaagcacata aacctggttc
    17641 agcgaccatc gcgcttaaca agcgcgcccg tcacgaatac tttatcgaag aagagttcga
    17701 agcgggactt gccctgcaag gctgggaagt taaatccctg cgcgcaggaa aagccaatat
    17761 cagcgacagc tacgtccttc tgcgtgacgg agaggcattt ctgtttggcg ctaacatcac
    17821 gccaatggcc gtggcctcca cgcatgtggt gtgcgatcct acccgtaccc gcaagttact
    17881 tctcaaccag cgcgaactgg actcattgta cggtcgcgtc aatcgagaag gctataccgt
    17941 agtggcgctc tccctgtact ggaaaaatgc ctggtgcaaa gtgaaaatcg gcgtcgccaa
    18001 aggtaaaaaa cagcacgata aacgttcaga tatcaaagaa cgcgaatggc aggtggataa
    18061 agcacgcatc atgaaaaacg cccaccgtta aacctgcact ccaattattg accagttcct
    18121 caccgcgcct ccctctccgg cggcgcgaat gaacatctta ttggctatca catccgacac
    18181 aaatgttgcc atcccattgc ttaatcgaat aaaaatcagg ctacatgggt gctaaatctt
    18241 taacgataac gccattgagg ctggtcatgg cgctcataaa tctggtatac ttacctttac
    18301 acattggggc tgattctgga ttcgacggga tttgcgaaac ccaaggtgca tgccgagggg
    18361 cggttggcct cgtaaaaagc cgcaaaaaat agtcgcaaac gacgaaaact acgctttagc
    18421 agcttaataa cctgcttaga gccctctctc cctagcctcc gctcttagga cggggatcaa
    18481 gagaggtcaa acccaaaaga gatcgcgtgg aagccctgcc tggggttgaa gcgttaaaac
    18541 ttaatcaggc tagtttgtta gtggcgtgtc cgtccgcagc tggcaagcga atgtaaagac
    18601 tgactaagca tgtagtaccg aggatgtagg aatttcggac gcgggttcaa ctcccgccag
    18661 ctccaccaaa attctccatc ggtgattacc agagtcatcc gatgaagtcc taagagcccg
    18721 cacggcgcaa gccctgcggg cttttttgtg ctccgaattt gtcccgcgaa gtctgatgcc
    18781 aactaattaa atccgatcct tttaggcacc ttgttaggca cttcataaag ctatattgtc
    18841 tttgaggtgc ctaaaactat ggaaacccgg caatggcaag acaaaccaaa cctctatccg
    18901 ttaaggaaat cgaatctgct aaacccaagg aagcggacta cgttctctat gatggcgatg
    18961 gccttgagct actcattaaa tccagcggga gtaaaatctg gcagtttcgc tacattcgcc
    19021 ctgtcaccaa gacacgagca aagaaaagca taggccctta cccgtcagtt acccttgccg
    19081 atgccagaaa ctaccgtgca gagtcccgct cactcctagc taaacagatt gatccgcagg
    19141 aacatcagca agaacaattg cgtagttcgc tggaagccaa aaccaatact ttccagctcg
    19201 tagctgaacg ttggtggaat gtgaagaaag ccagtgtgac agaggactat gcggaagata
    19261 tctggcgctc tcttgaaaga gatgtctttc ctgcgattgg cgacgttagc gttacagata
    19321 ttaaagctca tacactggtt caggccgtac aaccggttca ggccagagga gcactggaaa
    19381 cggttcgtcg cctgtgtcaa cgtattaatg aggtcatgat ctatgcccag aacacagggc
    19441 tgattgatgc tgttcccagc gttaatatcg gtaaagcctt cgaaaagcct cagaaaaaga
    19501 acatgcccag cattcgaccg gatcagctat ctcaactgat gcagacaatg cgaacagcca
    19561 gcattagcct ttccacacgc tgcctgttca tgtggcaact tcttactatt acccgccctg
    19621 ccgaagcggc tgaagctcgc tgggaagaag tagacataga agcgcaagag tggaagattc
    19681 ccgcagcacg catgaaaatg aaccgcgacc atactgttcc attgtcagat gaagcaatag
    19741 ctgtgctgga gatgatgaaa ccgctaagcg gcaaccgaga atttatcttt cctagccgta
    19801 tcaagcccaa ccagcctatg aacagtcaga ctgttaacgc ctcactaaaa cgcgcaggtt
    19861 ttggtggtgt gcttgtttcg catggtctga gatcaattgc cagtacggct cttaatgaac
    19921 aaggctttcc gcctgatgcc attgaggcag cattggccca tgtggataag aatgaggttc
    19981 gtcgtgctta taaccgcagc gattacctgg aacagcgtcg cccgatgatg caatggtggg
    20041 caaattttgt gatggcagcg gatcgtggaa gcatgattga aggtgggata aaaggaatga
    20101 agctagttgg gtgactaaat agctgcgcgg catagtgtcc cactttcagc cagcccttgc
    20161 aggttaagcc accagacagt aaacggcggg tgtcgcgtta ttaggcgcag ctatttagta
    20221 gtaatattag gattggtgat tctgcac
//
LOCUS       DAJTRC010000072        20080 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.072, whole genome shotgun sequence.
ACCESSION   DAJTRC010000072 DAJTRC010000000
VERSION     DAJTRC010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 20080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.072"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..610)
                     /gene="aroD"
                     /locus_tag="OO357_002770"
     CDS             complement(<1..610)
                     /gene="aroD"
                     /locus_tag="OO357_002770"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HCR5887660.1"
                     /translation="MKTVTVKDLVIGAGAPKIIVSLMAKDIARVKSEALAYREADFDI
                     LEWRVDHFADLSNVESVMAAAKILRETMPEKPLLFTFRSAKEGGEQAISTEAYIALNR
                     AAIDSGLVDMIDLELFTGDDQVKETVAYAHAHDVKVVMSNHDFHKTPEAEEIIARLRK
                     MQSFDADIPKIALMPQSTSDVLTLLAATLEMQEQYADRPIITM"
     gene            complement(641..1507)
                     /gene="ydiB"
                     /locus_tag="OO357_002771"
     CDS             complement(641..1507)
                     /gene="ydiB"
                     /locus_tag="OO357_002771"
                     /EC_number="1.1.1.282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinate/shikimate dehydrogenase"
                     /protein_id="HCR5887661.1"
                     /translation="MDVTAKYELIGLMAYPIRHSLSPEMQNKALEKAGLPFTYMAFEV
                     DNDSFPGAIEGLKALKMRGTGVSMPNKQLACEYVDELTPAAKLVGAINTIVNDDGYLR
                     GYNTDGTGHIRAIKESGFDIKGKTMVLLGAGGASTAIGAQGAIEGLKEIKLFNRRDEF
                     FDKALAFAQRVNENTDCVVTDTDLADQQAFAEALASADILTNGTKVGMKPLENESLVN
                     DISLLHPGLLVTECVYNPHMTKLLQQAQQAGCKTIDGYGMLLWQGAEQFTLWTGKDFP
                     LEYVKQVMGFGA"
     gene            complement(1519..2791)
                     /locus_tag="OO357_002772"
                     /pseudo
     CDS             complement(1519..2791)
                     /locus_tag="OO357_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310425.2"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            complement(2974..4179)
                     /locus_tag="OO357_002773"
     CDS             complement(2974..4179)
                     /locus_tag="OO357_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5887662.1"
                     /translation="MKNPYYPTALGLYFNYLVHGMGVILMSLNMASLETLWQTNAAGV
                     SIVISSLGIGRLSVLLFAGLLSDRFGRRPFIMLGMCCYMAFFFDILQTNNIIIAYVFG
                     FLAGMANSFLDAGTYPSLMEAFPRSPGTANILIKAFVSSGQFLLPLIISLLVWAELWF
                     GWSFMIAAGIMFINALFLYRCTFPPHPGRRLPVIKKTTSSTEHRCSIIDLASYTLYGY
                     ISMATFYLVSQWLAQYGQFVAGMSYTMSIKLLSIYTVGSLLCVFITAPLIRNTVRPTT
                     LLMLYTFISFIALFTVCLHPTFYVVIIFAFVIGFTSAGGVVQIGLTLMAERFPYAKGK
                     ATGIYYSTGSIATFTIPLITAHLSQRSIADIMWFDTAIGFLLALFIGLRSRKKTRHHS
                     LKENVAPGG"
     gene            complement(4279..4635)
                     /locus_tag="OO357_002774"
     CDS             complement(4279..4635)
                     /locus_tag="OO357_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707588.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiL family protein"
                     /protein_id="HCR5887663.1"
                     /translation="MNAYELQALRHIFAMTIDECATWIAQTGDSESWRQWENGKCAIP
                     DCVVEQLLAMRQQRKKHLHAIIEKINNRIGNNTMRFFPDLTAFQQVYPDGNFIDWKIY
                     QSVAAELYAHDLERLC"
     gene            complement(4771..4878)
                     /gene="rprA"
                     /locus_tag="OO357_002775"
     ncRNA           complement(4771..4878)
                     /ncRNA_class="antisense_RNA"
                     /gene="rprA"
                     /locus_tag="OO357_002775"
                     /product="antisense sRNA RprA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00034"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00034"
     gene            complement(5064..6176)
                     /gene="ydiK"
                     /locus_tag="OO357_002776"
     CDS             complement(5064..6176)
                     /gene="ydiK"
                     /locus_tag="OO357_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter YdiK"
                     /protein_id="HCR5887664.1"
                     /translation="MVNVRQPRDVAQILLSVLFLAIMIVACLWIVQPFILGFAWAGTV
                     VIATWPVLLRLQKIMFGRRSLAVLVMTLLLVMVFIIPIALLVNSIVDGSGPLIKAISS
                     GDMTLPDLAWLNTIPVIGAKLYAGWHNLLDMGGTAIMAKVRPYIGTTTTWFVGQAAHI
                     GRFMVHCALMLLFSALLYWRGEQVAQGIRHFATRLAGVRGDAAVLLAAQAIRAVALGV
                     VVTALVQAVLGGIGLAVSGVPYATLLTVLMILSCLVQLGPLPVLIPAIIWLYWTGDTT
                     WGTVLLVWSGVVGTLDNVIRPMLIRMGADLPLILILSGVIGGLIAFGMIGLFIGPVLL
                     AVSWRLFAAWVEEVPPPTDQPEEILEELGEIEKPNK"
     gene            6565..9621
                     /locus_tag="OO357_002777"
     CDS             6565..9621
                     /locus_tag="OO357_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HCR5887665.1"
                     /translation="MIPQISLAPGVVQLVLNFLQELEQQGFTGDAATSYADRLTMSTD
                     NSIYQLLPDAVVFPRSTADVALIARLAAQERYSSLIFTPRGGGTGTNGQALNQGIIVD
                     MSRHMNRIIEINPEEGWVRVEAGVIKDQLNQYLKPFGYFFAPELSTSNRATLGGMINT
                     DASGQGSLVYGKTSDHVLGVRAVLLGGDILDTQPLPVELAETLGKSNTTIGRIYNTVY
                     QRCRQQRQLIIDNFPKLNRFLTGYDLRHVFNDEMTEFDLTRILTGSEGTLAFITEARL
                     DITRLPKVRRLVNVKYDSFDSALRNAPFMVEARALSVETVDSKVLNLAREDIVWHSVS
                     ELITDMPDKEMLGLNIVEFAGDDEALIDERVNALCVRLDELIASQQAGVIGWQVCREL
                     AGVERIYAMRKKAVGLLGNAKGAAKPIPFAEDTCVPPEHLADYIAEFRALLDSHGLSY
                     GMFGHVDAGVLHVRPALDMCDPQQEILMKQISDDVVALTAKYGGLLWGEHGKGFRAEY
                     SPAFFGEELFAELRKVKAAFDPHNRLNPGKICPPEGLDAPMMKVDAVKRGTFDRQIPI
                     AVRQQWRGAMECNGNGLCFNFDARSPMCPSMKITQNRIHSPKGRATLVREWLRLLADR
                     GVDPLKLEQELPESGVSLRTLIARTRNSWHANKGEYDFSHEVKEAMSGCLACKACSTQ
                     CPIKIDVPEFRSRFLQLYHTRYLRPLRDHLVATVESYAPLMARAPKTFNFFINQPLVR
                     KLSEKHIGMVDLPLLSVPSLQQQMVGHRSANMTLEQLEALNAEQKARTVLVVQDPFTS
                     YYDAQVVADFVRLVEKLGFQPVLLPFSPNGKAQHIKGFLNRFAKTAKKTADFLNRMAK
                     LGMPMVGVDPALVLCYRDEYKLALGEERGEFNVLLANEWLASALESHPVATVSGESWY
                     FFGHCTEVTALPGAPAQWAAIFARFGAKLENVSVGCCGMAGTYGHEAKNHENSLGIYE
                     LSWHQAMQRLPRNRCLATGYSCRSQVKRVEGTGVRHPVQALLEIIK"
     gene            9618..10028
                     /gene="menI"
                     /locus_tag="OO357_002778"
     CDS             9618..10028
                     /gene="menI"
                     /locus_tag="OO357_002778"
                     /EC_number="3.1.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA hydrolase"
                     /protein_id="HCR5887666.1"
                     /translation="MIWKRKITLEALNAMGEGNMVGFLDIRFEHIGDDTLEATMPVDS
                     RTKQPFGLLHGGASVVLAESIGSVAGYLCTEGEQKVVGLEINANHVRSAREGRVRGIC
                     KPLHLGSRHQVWQIEIFDEKGRLCCSSRLTTAIL"
     gene            10128..10316
                     /locus_tag="OO357_002779"
     CDS             10128..10316
                     /locus_tag="OO357_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416200.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiH family protein"
                     /protein_id="HCR5887667.1"
                     /translation="MSTQLDPTQLAIEFLRRDQSNLSPAQYLKRLKQLELEFADLLTL
                     SSAELKEEIYFAWRLGVH"
     gene            10864..11232
                     /gene="sufA"
                     /locus_tag="OO357_002780"
     CDS             10864..11232
                     /gene="sufA"
                     /locus_tag="OO357_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly scaffold SufA"
                     /protein_id="HCR5887668.1"
                     /translation="MDMHSGTFNPQDFAWQGLTLTPAAAIHIRELVAKQPGMVGVRLG
                     VKQTGCAGFGYVLDSVSEPDKDDLLFEHDGAKLFVPLQSMPFIDGTEVDFVREGLNQI
                     FKFHNPKAQNECGCGESFRV"
     gene            11241..12728
                     /gene="sufB"
                     /locus_tag="OO357_002781"
     CDS             11241..12728
                     /gene="sufB"
                     /locus_tag="OO357_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HCR5887669.1"
                     /translation="MSRNTEATDDVKTWTGGPLNYKEGFFTQLATDELAKGINEEVVR
                     AISAKRNEPEWMLEFRLNAYRAWLEMEEPHWLKAHYDKLNYQDYSYYSAPSCGNCDDT
                     CASEPGAVQQTGANAFLSKEVEAAFEQLGVPVREGKEVAVDAIFDSVSVATTYREKLA
                     EQGIIFCSFGEAIHDHPELVRKYLGTVVPGNDNFFAALNAAVASDGTFIYVPKGVRCP
                     MELSTYFRINAEKTGQFERTILVADEDSYVSYIEGCSAPVRDSYQLHAAVVEVIIHKN
                     AEVKYSTVQNWFPGDNNTGGILNFVTKRALCEGENSKMSWTQSETGSAITWKYPSCIL
                     RGDNSIGEFYSVALTSGHQQADTGTKMIHIGKNTKSTIISKGISAGHSQNSYRGLVKI
                     MPTATNARNFTQCDSMLIGANCGAHTFPYVECRNNSAQLEHEATTSRIGEDQLFYCLQ
                     RGISEEDAISMIVNGFCKDVFSELPLEFAVEAQKLLAISLEHSVG"
     gene            12738..13484
                     /gene="sufC"
                     /locus_tag="OO357_002782"
     CDS             12738..13484
                     /gene="sufC"
                     /locus_tag="OO357_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008500629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HCR5887670.1"
                     /translation="MLSIKDLHVSVEDKAILRGLSLDVRPGEVHAIMGPNGSGKSTLS
                     ATLAGREDYEVTGGTVEFKGKDLLALSPEDRAGEGIFMAFQYPVEIPGVSNQFFLQTA
                     LNAVRSYRGQETLDRFDFQDLMEEKIALLKMPEDLLTRSVNVGFSGGEKKRNDILQMA
                     VLEPELCILDESDSGLDIDALKVVADGVNSLRDGKRSFIIVTHYQRILDYIKPDYVHV
                     LYQGRIVKSGDFTLVKQLEEQGYGWLTEQQ"
     gene            13459..14730
                     /gene="sufD"
                     /locus_tag="OO357_002783"
     CDS             13459..14730
                     /gene="sufD"
                     /locus_tag="OO357_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HCR5887671.1"
                     /translation="MAGLPNSSNALQQWHHLFEAEGTKRSPQAQQHLQQLLRTGLPTR
                     KHENWKYTPLEGLTNSQFVSIAGDISPQQRDALALTLDAVRLVFVDGRYVPALSDAIE
                     GSGYEVSINDDRQGLPDAIQAEVFLHLTESLAQSVTHIAVKRGQRPAKPLLLMHITQG
                     VAGEEVNTAHYRHHLDLAEGAEATVIEHFVSLNDARHFTGARFTINVAANAHLQHIKL
                     AFENPVSHHFAHNDLLLADDATAFSHSFLLGGAVLRHNTSTQLNGENSTLRINSLAMP
                     VKNEVCDTRTWLEHNKGFCNSRQLHKTIVSDKGRAVFNGLINVAQHAIKTDGQMTNNN
                     LLMGKLAEVDTKPQLEIYADDVKCSHGATVGRIDDEQMFYLRSRGINQQDAQQMIIYA
                     FAAELTEALRDEGLKQQVLARIGQRLPGGAR"
     gene            14727..15947
                     /gene="sufS"
                     /locus_tag="OO357_002784"
     CDS             14727..15947
                     /gene="sufS"
                     /locus_tag="OO357_002784"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase SufS"
                     /protein_id="HCR5887672.1"
                     /translation="MTFSVDKVRADFPVLSREVNGLPLAYLDSAASAQKPSQVIDAEA
                     EFYRHGYAAVHRGIHTLSAQATEKMENVRKRASLFINARSAEELVFVRGTTEGINLVA
                     NSWGNSNVRAGDNIIISQMEHHANIVPWQMLCARVGAELRVIPLNPDGTLQLETLPTL
                     FDEKTRLLAITHVSNVLGTENPLAEMITLAHQHGAKVLVDGAQAVMHHPVDVQALDCD
                     FYVFSGHKLYGPTGIGILYVKEALLQEMPPWEGGGSMIATVSLSEGTTWTKAPWRFEA
                     GTPNTGGIIGLGAALEYVSALGLNSIAEYEQNLMHYALSQLESVPDLTLYGQQNRLGV
                     IAFNLGKHHAYDVGSFLDNYGIAVRTGHHCAMPLMAYYNVPAMCRASLAMYNTHEEVE
                     RLVTGLQRIHRLLG"
     gene            15960..16376
                     /gene="sufE"
                     /locus_tag="OO357_002785"
     CDS             15960..16376
                     /gene="sufE"
                     /locus_tag="OO357_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfuration protein SufE"
                     /protein_id="HCR5887673.1"
                     /translation="MALLPDKEKLLRNFLRCANWEEKYLYIIELGQRLPELRAEDGSP
                     QNSIQGCQSQVWIVMRQNAQGIIELQGDSDAAIVKGLIAVVFILYDQMTPQDIVNFDV
                     RPWFEKMALTQHLTPSRSQGLEAMIRAIRAKAAALS"
     gene            16525..17529
                     /gene="ldtE"
                     /locus_tag="OO357_002786"
     CDS             16525..17529
                     /gene="ldtE"
                     /locus_tag="OO357_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase LdtE"
                     /protein_id="HCR5887674.1"
                     /translation="MKRASLLTLTLIGAFSAIQAAWAVDYPLPPTGSRLVGQNQTYTV
                     QEGDKNLQAIARRFDTAAMLILEANNTIAPVPKPGTTITIPSQLLLPDAPRQGIIVNL
                     AELRLYYYPPGENIVQVYPIGIGLQGLETPVMETRVGQKIPNPTWTPTAGIRQRSLER
                     GIKLPPVVPAGPNNPLGRYALRLAHGNGEYLIHGTSAPDSVGLRVSSGCIRMNAPDIK
                     ALFSSVRTGTPVKVINEPVKYSVEPNGMRYVEVHRPLSAEEQQNVQTMPYTLPAGFTQ
                     FKDNKAVDQKLVDKALYRRAGYPVSVSSGATPAASNAPSVESAQNGEPEQGNMLRATQ
                     "
     gene            complement(17592..17828)
                     /gene="lpp"
                     /locus_tag="OO357_002787"
     CDS             complement(17592..17828)
                     /gene="lpp"
                     /locus_tag="OO357_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein lipoprotein Lpp"
                     /protein_id="HCR5887675.1"
                     /translation="MKATKLVLGAVILGSTLLAGCSSNAKIDQLSSDVQTLNAKVDQL
                     SNDVNAMRSDVQAAKDDAARANQRLDNMATKYRK"
     gene            complement(18139..19551)
                     /gene="pykF"
                     /locus_tag="OO357_002788"
     CDS             complement(18139..19551)
                     /gene="pykF"
                     /locus_tag="OO357_002788"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010346010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase PykF"
                     /protein_id="HCR5887676.1"
                     /translation="MKKTKIVCTIGPKTESEEMLAKMLDAGMNVMRLNFSHGDYAEHG
                     QRIQNLRNVMSKTGKTAAILLDTKGPEIRTMKLEGGNDVSLKAGQTFTFTTDKSVIGN
                     SEMVAVTYEGFTTDLSVGNTVLVDDGLIGMEVTAIEGNKVICKVLNNGDLGENKGVNL
                     PGVSIALPALAEKDKQDLIFGCEKGVDFVAASFIRKRSDVIEIREHLKAHGGENIHII
                     SKIENQEGLNNFDEILEASDGIMVARGDLGVEIPVEEVIFAQKMMIEKCIRARKVVIT
                     ATQMLDSMIKNPRPTRAEAGDVANAILDGTDAVMLSGESAKGKYPLEAVSIMATICER
                     TDRVMNSRLEFNNDNRKLRITEAVCRGAVETAEKLDAPLIVVATQGGKSARAVRKYFP
                     DATILALTTNEKTAHQLVLSKGVVPQLVKEITSTDDFYRLGKELALQSGLAHKGDVVV
                     MVSGALVPSGTTNTASVHVL"
BASE COUNT         5260 a         4814 c         5133 g         4873 t
ORIGIN      
        1 acatcgtgat gattggacga tcggcatact gctcctgcat ctccagggtc gcggcaagca
       61 acgtcagcac atcgctggta ctttgcggca tcagcgcaat cttaggaata tcggcgtcga
      121 aggactgcat tttgcgcaga cgggcaatga tttcttcggc ttccggcgtt ttatggaagt
      181 catggttgga catgacaact ttcacatcat gcgcgtgggc gtaggcgacg gtttctttga
      241 cctgatcatc gccggtaaat aactccagat cgatcatatc aaccaggccg ctgtcgatgg
      301 ctgcacgatt gagagcaata taagcctcgg tggaaatcgc ctgctcgccg ccttctttgg
      361 cactgcggaa ggtaaacagc agcggttttt ctggcatggt ttcacggaga atttttgccg
      421 ccgccatgac agactccaca ttggagaggt cggcaaagtg gtccacacgc cattccagaa
      481 tatcaaagtc cgcttcacga taggcgagag cttcggattt cacgcgggcg atatctttcg
      541 ccatcagcga gacgatgatt ttaggtgcgc ccgcaccaat gacgagatct tttacagtta
      601 cggttttcat tttttaccct ttctgcacgc ggtcagcctg tcaggcaccg aaccccatga
      661 cctgtttaac atattccaga gggaaatctt tgccagtcca taacgtgaac tgttcagccc
      721 cttgccacaa caacatgccg tatccatcaa tcgttttgca accagcttgt tgtgcctgct
      781 gcaataactt cgtcatatgc gggttataca cgcattcagt gaccagcagt cccggatgta
      841 acagactgat atcattaacc aatgattcat tctcaagggg tttcatacct acttttgtgc
      901 cattggttaa aatgtcggcg gaagccaggg cttcagcaaa ggcttgctga tcggcgagat
      961 cggtatccgt gacgacacaa tcggtgtttt cgttaacccg ctgcgcgaag gcgagggctt
     1021 tatcgaagaa ctcatcccga cggttaaaga gtttaatttc ttttaaacct tcaattgccc
     1081 cctgcgcgcc aatcgccgtt gaggcacctc cggcccctaa cagcaccatc gttttgcctt
     1141 tgatatcaaa accgctctct ttaatggcgc gaatatggcc cgtgccgtcg gtgttatagc
     1201 cacgcagata gccatcatca ttaacgatgg tgttgatggc acccaccagt ttggctgctg
     1261 gcgttaattc atcaacatat tcacacgcca gttgtttgtt cggcatcgac acaccagttc
     1321 cgcgcatttt gagggctttt aatccttcaa ttgctccggg aaagctatcg ttatccactt
     1381 cgaaggccat ataggtaaat ggcaatcccg ctttttctaa ggctttattc tgcatttcag
     1441 gcgataaact gtggcggata ggataagcca tcaatccaat caattcgtat tttgcggtaa
     1501 catccataat taactccttt aacctctatg cttaattgtg tttaaccggg tgctaaattt
     1561 gcgttcgcca aaccgcacat cattctcagg aataataaaa acgcggtaat agcggctaaa
     1621 aacaataatt gcggtaatca gggccagcag cgcgaaagta aaatcgagaa caatgatata
     1681 ttgcaggccg atgttcgaca gataaccggt aatcagcgga ataacaaagt tagccagtcc
     1741 acccatcatc atataaatac tggtgacttt ggctttgctt ttggggaaaa actctgacat
     1801 gaccgaaaca ccgagctgta aaatgccgcc agctgctgag aaaccgataa caaaggcgcc
     1861 ggcattacac accagtggag aagggtacag gtagataatg gcagctgtta ttgttgccag
     1921 cgcagagtta aatacattag cccagatggg ccggaccatt tttttcagta gtgcggcaaa
     1981 aataaagaca cagaccaacg agcccatact gtaataagag atggttttta atgcctcagc
     2041 ttctgacata ccagcaaaag ccatcgcata tttgggcatc cacaccacaa tcacataaaa
     2101 ggtcgagaat gcggctacac cgaacagtac cgatgaaaca ccttccagcc agactaaagg
     2161 ttttgctgtt catttgcggt aattcattag ctacgctggc gtccaccaac tggctgggga
     2221 atttgctttt caacagcatc agcgtgatca gtacaaacag aatacccgga ataatcagcc
     2281 catagccgta ccagatatta ttgagcaaca tatagctcac cagcattggg tagaacattt
     2341 gcccaaatga caccatcgct ttaaccagta tgaccgccga accagaggct ttcggaaagc
     2401 attccatgag cgcggggtag ccacccgtat ccagcgctga gttagcgata cctacgcaca
     2461 ctgccagacc gtaggcgaga gttaaattcg ggcaagcggg aataccaaag aagaatagca
     2521 gatacattat tactgccatt aatatcaccg cccgacgacc aaacttatcg gagatcacac
     2581 cgaagaataa aatactgatc aatcgcccca aaccgatacc ggaaattaaa taggcaatgc
     2641 ccgcgttgtc agtggaaaac ttttctgaca gagatgtcat attttgggca agcgtaataa
     2701 cactaatgcc gtgcaggaaa tagctgaagt aaatacaaag aacagccagg ataaatggcg
     2761 tgctgaaaga taatttcgtt accgtacgta ttgttctccg acggtttaca taattactat
     2821 actgaagatg attttccctg ggtacatggc ataaagttta tgtttccagg aatattgcat
     2881 gtcatagttt gtttttgatc gcgattttcc ttagttagcg cagaaagagt tatttatccg
     2941 taagatcttg catgacctga aaagaatatt gcattaccca cccggagcga cattttcctt
     3001 tagcgagtga tgccgcgttt ttttgcggct gcgtaagccg ataaacagtg ccagtaaaaa
     3061 accgatggca gtatcgaacc acataatatc ggcaatactt ctttgggaca gatgagccgt
     3121 aatcaacgga atagtaaagg tcgcaatact gcccgtactg taatagatcc ctgtagcttt
     3181 acctttagcg taagggaaac gttcagccat taacgtcagg ccaatttgca caacacctcc
     3241 agcagaggta aaaccaatga caaaagcaaa tattatcacc acataaaatg tgggatgcag
     3301 gcagacggta aacagagcaa taaatgagat aaaggtgtac agcatcagta atgttgttgg
     3361 gcgaacggta ttacgaatga gtggagcggt aataaataca caaagcagcg aacccacggt
     3421 gtagatactg agtagtttga tcgacatagt gtatgacatg cctgcaacaa attgtccgta
     3481 ctgtgccagc cactggctaa ccagataaaa cgttgccatt gagatatagc catataaggt
     3541 ataactggct aaatcgataa ttgaacagcg atgttccgta gagctggtgg ttttctttat
     3601 gacaggtaag cgacgacccg gatggggtgg gaacgtacaa cggtataaaa acagagcgtt
     3661 aataaacata atgcctgcag caatcataaa ggaccaaccg aaccacagtt cagcccacac
     3721 taacaggcta atgattagcg gtaataaaaa ttgtccgctg gaaacaaatg ctttaattaa
     3781 aatattggct gtcccaggtg agcgtggaaa agcttccatc aaactgggat aagtgcctgc
     3841 atcgagaaaa ctgtttgcca ttcccgccag aaagccaaaa acataagcga taatgatgtt
     3901 attggtctgc aggatgtcaa aaaagaaggc catatagcag cacatcccga gcatgataaa
     3961 agggcggcga ccaaagcgat cggataataa tcctgcaaaa agcaagacac ttaatcgacc
     4021 aatgcccagc gatgagataa ctatcgagac acccgcggca ttagtctgcc aaagtgtctc
     4081 cagcgaggcc atattcaggc tcatcaaaat gacgcccata ccatgcacca ggtaattaaa
     4141 atacaaccca agtgcggtag ggtaataggg atttttcata ggttctactt agcaaaggtg
     4201 ctttaaaaag tgtagcgatg ttgtgtcagt atccattcat tattgttgta acaacgacaa
     4261 ttcctttctg tggaaaaatt aacaaagccg ctccagatcg tgtgcatata attcagcggc
     4321 taccgattga tagatcttcc agtcaataaa attgccatca gggtagactt gttgaaacgc
     4381 ggttaagtcg gggaaaaaac gcatggtgtt gttgccgatg cggttattta ttttctctat
     4441 gatggcatga agatgttttt ttcgttgctg acgcatagcc aacagttgct cgactacaca
     4501 atcaggaatg gcgcatttgc cattttccca ctgacgccag ctttcactgt caccggtctg
     4561 ggcaatccag gtcgcgcatt catcaatagt catggcaaaa atatggcgga gtgcctggag
     4621 ttcataagcg ttcatcgtgt aatggctgca tgaaataaac aataaaatat aaataacatt
     4681 atcagcctgc tgacggcttg aagagagtca cagtatcttg tgcaacatta ttggaaagag
     4741 tgaggggcga ggtagcgaag cggaaaaatg ttaaaaaaaa gcccatcgtg ggagatggga
     4801 aaagactaca cacagcaatt cgttgtttca ctcaggggat ttccatgctt ataaatcaat
     4861 atgttgattt ataaccgtga gctaatagta ggcatacgga ctttttgcgt cgatcagatt
     4921 cgtctcatta gtttaaaaac tgtaaagaat ttgcgacagg tactaccagc tgaatggcgg
     4981 taagcaggaa aatatgggga agagcaagca aatatcaatt agcggtgatt aacaatcagg
     5041 cggcagagcc gcctgatgag aaattactta ttcggtttct cgatttcgcc aagttcttcg
     5101 agaatttctt ccggttggtc cgtcggtggc gggacttctt ccacccacgc ggcaaacaga
     5161 cgccaggaaa cggctaacag aaccggacca ataaacagac cgatcatccc gaaagcaatc
     5221 aaaccaccaa taacgccaga gaggatcagg atcagcggta aatcggcacc catgcgaatt
     5281 aacattgggc ggatgacgtt atccagcgtg ccaaccacac cgctccacac taacaatacc
     5341 gttccccagg tggtatcgcc ggtccagtag agccagataa tcgccggaat cagtaccggc
     5401 aacgggccaa gctggacaag gcaggagagg atcattaaca ccgttagcaa cgttgcataa
     5461 ggtacgccgg atacggcgag gccgataccg ccaagcactg cctgtactaa cgccgtcacc
     5521 accacaccca gcgccaccgc gcggatagcc tgtgccgcca gcagcacggc ggcatctccg
     5581 cgaacgcctg ccagacgggt tgcaaaatgg cgaatgcctt gtgccacctg ttcaccgcgc
     5641 cagtacagca gggcactgaa gagaagcatc agcgcacaat gcaccataaa gcgcccgata
     5701 tgcgccgcct gcccaacgaa ccaggtggtg gtggtgccaa tataagggcg gactttcgcc
     5761 atgatcgccg taccccccat atccagcaag ttgtgccagc ctgcatacag cttcgcgcca
     5821 atcaccggaa tggtattaag ccacgccaga tcgggtaacg tcatgtcgcc gctggaaatg
     5881 gctttaatta gcgggccgct gccgtcgacg atactgttaa ccagcaaagc aatagggatg
     5941 ataaacacca tcactaataa cagcgtcatc accagaacgg cgagagagcg gcggccaaac
     6001 atgatctttt gcaaacgtaa caataccggc caggtggcga taaccaccgt accggcccat
     6061 gcaaagccga gaataaaggg ttgaacaatc cacagacatg ccacaatcat gatggctaaa
     6121 aacagcaccg aaagcagaat ttgtgcgaca tccctgggct gacgaacatt taccataact
     6181 acttttcacc ttttttgtgc gtcaaatcgt cgacgcgacg tgaacacgcg aaactcacct
     6241 gcataatgat gagcaatttc agcggttttt aacaggccga ttctgccctt aattctgatg
     6301 ggcgtgttag aaaaaaatgt ggtacaacaa ctcaggcaac acgcaaacga tttactcgtc
     6361 gtatttcaag ttgcatgtgc tgcggctacg ttcgttcacc ccagtcactt actattgtaa
     6421 gctcctggag ttcactcgct tgccgccttc ctgcaactcg aaatccatag agtaaataaa
     6481 catcaccttt cagtgcgcta ttgcagccgc ctgaaagaat agtgtatcga cctacatcac
     6541 agaccgcagg aaagggtcaa tataatgatt ccacagattt ccctggcacc cggcgtcgtt
     6601 caactggtgc ttaatttttt gcaagagctg gagcaacaag gttttaccgg cgatgcggcg
     6661 acaagttatg ccgatcgtct gacaatgtcg accgacaaca gtatttacca acttctcccc
     6721 gatgcggtgg tatttccgcg ttcaaccgca gatgtggcgc tgatcgcccg tcttgccgcg
     6781 caggaacgct attcatcgct gatctttacc ccccgcggcg gcggcaccgg cactaacggt
     6841 caggcgctca accaggggat tattgttgat atgtcccgcc atatgaaccg catcatcgaa
     6901 attaaccctg aagagggctg ggtgcgcgtt gaggccgggg tgataaaaga ccaactcaat
     6961 cagtacctga aaccgttcgg ctactttttt gcgccggaac tttcgaccag caaccgggca
     7021 acgctcggtg ggatgatcaa taccgatgca tccggtcagg gatcgctggt ctatggcaaa
     7081 acgtcagatc acgtacttgg cgtacgcgcg gtgttgttgg ggggcgatat tctcgatacg
     7141 caacctttac ccgtcgagct ggcggaaacg ctgggtaaat ccaataccac aatcgggcga
     7201 atttataaca cggtttatca acgttgccgt cagcaacgcc agttaatcat cgacaacttc
     7261 cccaaactta accgctttct taccggttac gatctgcgtc atgtctttaa cgatgagatg
     7321 accgagttcg acctgacgcg cattctgacg ggttcagaag ggacgctggc ctttattacc
     7381 gaagcgcggc tggatattac gcgcttgcct aaagtgcgcc gtctggtgaa tgtcaaatat
     7441 gactcttttg actctgcgct gcgtaacgcg ccgtttatgg ttgaggcgcg ggcgctttcg
     7501 gtagagacgg tggactcaaa agtgctgaat ctggcgcggg aagatattgt ctggcattcc
     7561 gtcagcgagt tgattaccga tatgcctgac aaagagatgc tcggactgaa cattgtggaa
     7621 tttgctggtg atgatgaggc gctgattgac gagcgagtta atgcactctg tgtgcggctt
     7681 gatgagctga ttgccagcca acaggcaggt gtgattggct ggcaggtgtg ccgcgagctg
     7741 gcgggggttg aacgtatcta tgcgatgcgc aaaaaagccg ttggtctgct tggcaatgcc
     7801 aaaggtgccg ctaagccaat tccgtttgct gaggatacct gcgtaccgcc ggaacatctg
     7861 gccgattata ttgctgaatt tcgcgcgctg ctcgacagcc acggcttaag ctacggtatg
     7921 ttcggtcacg tcgacgcagg tgtcttgcac gtccgtccgg cactggatat gtgcgatccg
     7981 caacaagaga ttttgatgaa gcaaatctct gatgacgtgg tggcgctgac tgcgaaatac
     8041 ggtggtttgt tgtggggcga gcacggcaaa ggttttcgcg ctgaatacag cccggcgttt
     8101 ttcggtgagg aactttttgc agaactgcgc aaagtgaaag cggcatttga cccgcataac
     8161 cgactcaacc cagggaagat ttgcccgcca gaaggtctcg atgcgccgat gatgaaagtg
     8221 gacgcggtga agcgcggtac attcgatcgg cagatcccca ttgcggtacg ccagcagtgg
     8281 cgcggtgcga tggagtgtaa cggcaacggt ttatgcttca actttgatgc ccgtagtccg
     8341 atgtgtccgt cgatgaagat cacccagaac cggattcatt caccgaaagg gcgcgcaacg
     8401 ctggtgcgtg aatggctgcg cttgttggcg gatcgcggcg ttgacccact caaactggaa
     8461 caagaactgc ctgaatccgg cgtcagtttg cggacgttaa ttgcccgcac gcgcaatagc
     8521 tggcatgcga ataaaggcga atatgacttc tcacacgaag tcaaagaggc gatgtcgggc
     8581 tgtctggcct gtaaagcttg ttcgacccag tgccccatca aaattgatgt gccggagttt
     8641 cgctctcgtt ttctgcagct ctatcacacc cgttatttac gcccgcttcg cgaccacctc
     8701 gttgctacgg tcgagagcta cgcaccgctg atggcacgcg cgccgaaaac ctttaacttc
     8761 ttcattaacc agccgctggt gcgcaaactc tcggaaaaac atatcggcat ggttgatttg
     8821 ccactgctgt cggtcccctc gctacaacaa caaatggtgg ggcatcgctc ggcaaacatg
     8881 acgctggaac agcttgaagc tctcaatgca gagcagaaag cgcgcacggt gttggtggtg
     8941 caggacccct ttaccagcta ttacgatgcg caagtggtgg cggattttgt ccgtctggtt
     9001 gaaaaattag gtttccagcc tgtgttactg ccattttcgc caaatggcaa agcccagcat
     9061 atcaaaggct ttcttaatcg ttttgcgaag acggcgaaaa agacggcgga tttcctcaac
     9121 cgtatggcta agcttgggat gccaatggtg ggcgtcgatc cggcgctggt actttgttat
     9181 cgcgatgaat ataaactggc gctgggcgag gaacgtggcg agtttaacgt cttacttgcg
     9241 aatgaatggc tggcaagcgc acttgagtca catccagtgg ctacagtcag cggtgaatca
     9301 tggtatttct ttggtcactg taccgaggtt accgctttgc cgggcgcgcc agcacaatgg
     9361 gccgcgatat ttgcccgttt tggcgcgaaa ctggaaaatg tcagcgtggg ttgctgcggc
     9421 atggcaggga cttacggaca tgaagcgaaa aaccatgaaa attcgctcgg gatctatgag
     9481 ttatcctggc atcaggctat gcagcgactg ccgcgtaacc gctgtctggc gaccggatat
     9541 tcctgccgta gccaggtaaa acgggttgaa ggcacggggg tacgccatcc tgtgcaggct
     9601 ttactggaga ttattaaatg atatggaaac ggaaaatcac cctggaagca ctgaatgcta
     9661 tgggtgaagg aaacatggtg gggttcctgg atattcgctt tgaacatatt ggtgatgaca
     9721 cccttgaagc gacaatgcca gtagactcgc ggacaaagca gcctttcggg ttgctgcatg
     9781 gaggagcatc cgtggtactg gccgaaagta tcggttccgt tgccggttat ttatgtaccg
     9841 aaggtgagca aaaagtggtt ggtctggaaa tcaatgctaa tcacgtccgc tcggcacgag
     9901 aagggcgggt gcgcggcata tgtaaaccgt tgcatctcgg ttcgcgtcac caggtctggc
     9961 agattgaaat cttcgatgag aaagggcgtt tgtgctgttc gtcacgattg acgaccgcca
    10021 ttttgtgacg ttgtcaccag aaaagtgtga cgcatattgc ttttggtcat tatctgcaca
    10081 gtaaacggtg gtatactgtg caggtcttgt ctaaaagcga ggatgctatg tctactcaac
    10141 ttgatcccac ccagctggcc attgaatttt tacgccgtga ccagagcaat ctttcacctg
    10201 cccaatatct caaacgtctg aaacaactag aacttgaatt tgctgacctg cttactctct
    10261 cttctgctga attaaaagaa gaaatctact tcgcctggcg tctgggcgtg cattgatcct
    10321 gacttaatgc cgcaagttcg ccaaatcgac ctggattaac ctccgtccag gttcgatgac
    10381 cttcttaata ctgcttatct accgcctgat attgttcaaa aataatacta atcgcagttt
    10441 gtgttaaaac ggcgggttag ctttatgaga ttattcttat cgccccttca agagctaagc
    10501 cactgagagt gccggagata agcgccggat ggggtagaaa cccttaagcc tgtgtcgcac
    10561 agacttaagg gttttcttat ttctgaacgc tgcacttttt tacctatctc ttaaatagtc
    10621 attttcaata caactttttc tttttcatca cagccttaat gctccttaga taataaccat
    10681 tatctaacaa tgagatacct aattcttagc gtgcctgtta acccacacat caggggctat
    10741 gcttattaaa agcaggcaaa aagaatgttt tacggtaaag cccctgcgtt tgctgggttg
    10801 aactgataat cattatcact aacatgctgt tatacgctga aagcgatgaa gtgaggtaaa
    10861 tagatggaca tgcattcagg aacctttaac ccacaggatt tcgcctggca aggcttaacg
    10921 ctgacacccg cagcggcgat acacatccgc gagctggtgg caaagcagcc aggtatggtc
    10981 ggcgtgcgct taggtgtgaa gcaaacgggc tgcgcgggct ttggctatgt gctcgacagt
    11041 gttagcgagc cggacaaaga cgatctgctg tttgaacacg acggcgcgaa gctgtttgtc
    11101 ccgctgcaat cgatgccgtt tattgatggc acggaagtcg atttcgttcg tgaaggactt
    11161 aatcagatat tcaaatttca caaccctaaa gcccagaatg aatgtggctg tggcgaaagc
    11221 tttagggtat aggcggtact atgtctcgta atactgaagc aactgacgat gtcaaaacct
    11281 ggaccggcgg cccgctgaac tataaagaag gattcttcac ccagttagcc accgatgagc
    11341 tggcaaaggg gataaacgaa gaggtggtgc gcgcaatttc ggcgaagcgt aatgagccgg
    11401 agtggatgct ggagtttcgt ctaaacgcct atcgcgcatg gctggagatg gaagaaccgc
    11461 actggttgaa agcgcactac gacaagctga attatcagga ttacagctac tactcagcac
    11521 catcgtgcgg taattgtgac gacacttgcg cgtctgaacc tggcgcggtg cagcaaactg
    11581 gcgcgaacgc ctttttaagt aaagaggtgg aggcggcgtt tgagcagttg ggcgttcccg
    11641 tgcgggaagg caaagaggtg gcggtggatg ccattttcga ctctgtttcg gttgccacca
    11701 cttatcgcga aaaactggcg gagcagggaa ttattttctg ttcctttggt gaggcgatcc
    11761 acgatcaccc ggaactggtg cgtaaatatc tcggcaccgt ggtgccgggg aatgacaact
    11821 tctttgcagc acttaatgcg gcggtagcct ctgacggtac gtttatttat gtgcctaaag
    11881 gcgtgcgctg cccgatggaa ctttccacct attttcgcat taacgcggaa aaaaccgggc
    11941 agtttgagcg cacaattctg gtggccgacg aagacagcta cgtcagctac attgaaggct
    12001 gttccgctcc ggtgcgtgac agctatcagt tgcacgcggc agtggtggaa gtcatcatcc
    12061 ataaaaacgc cgaggtgaaa tattccacgg tacaaaactg gttccctggc gataacaaca
    12121 ccggcggtat tctcaacttc gtcaccaagc gtgctttgtg cgaaggcgaa aacagcaaaa
    12181 tgtcatggac gcaatcagaa accgggtcag cgattacgtg gaaatatccc agctgcattt
    12241 tgcgcggcga taactccatt ggtgagtttt actcagtggc gctgaccagc ggtcatcagc
    12301 aagcggatac cggcaccaag atgatccaca tcggtaaaaa caccaaatcg accattatct
    12361 cgaaagggat ctctgccgga catagtcaga acagttatcg cggcttagtg aaaatcatgc
    12421 cgacggcaac caatgcgcgc aatttcactc agtgcgactc aatgctgatt ggcgctaatt
    12481 gtggggcgca taccttcccg tatgtcgaat gtcgcaataa cagcgcacaa ctggagcacg
    12541 aggcaacgac atcacgtatt ggtgaagatc aactgtttta ctgcctgcaa cgcgggatca
    12601 gcgaagaaga cgccatctcg atgattgtta acggtttctg caaagacgtg ttctcagaac
    12661 tgccgctgga atttgccgtt gaagcacaaa aactcctcgc catcagtctt gaacacagcg
    12721 tcggataagg aataaacatg ttaagtatta aagatttaca cgtcagcgtg gaagataaag
    12781 ctatcctgcg tggattaagc ctcgacgttc gtcccggcga agttcacgcc attatggggc
    12841 caaacggttc gggcaaaagt accttatcgg caacgcttgc cgggcgcgaa gattatgaag
    12901 tgacgggcgg cacggttgag ttcaaaggca aagatttgct tgcactgtcg ccggaagatc
    12961 gtgcaggcga aggcatcttt atggccttcc agtatccggt ggagattcca ggcgtcagta
    13021 accagttttt cctgcaaacg gcacttaatg cagtgcgcag ctatcgcggc caggaaacgc
    13081 tcgaccgctt tgattttcag gatttgatgg aagagaaaat cgctctcctg aagatgccgg
    13141 aagatttatt aacccgttcg gtaaacgttg gtttttccgg cggcgagaaa aagcgcaacg
    13201 atattttgca aatggcggtg ctggaaccgg agttatgcat tcttgatgag tcggactccg
    13261 ggctggatat tgacgcatta aaagtggtcg ccgatggcgt gaactcgctg cgtgatggca
    13321 agcgctcatt catcattgtt acgcactacc aacgcattct cgactacatc aagcctgatt
    13381 acgttcatgt gctatatcag ggacgaattg tgaaatccgg cgatttcacg ttggtcaaac
    13441 aactggagga gcagggttat ggctggctta ccgaacagca gtaatgcgct gcaacagtgg
    13501 catcacttgt ttgaagctga agggacaaaa cgctcaccgc aagcacagca gcatttacaa
    13561 caattgctgc gtaccggact gccgacacgt aaacatgaaa actggaaata tacgccgctg
    13621 gaagggctga ccaatagtca gtttgtcagc attgcgggag atatatcccc acagcagcgt
    13681 gatgccttag cgttaacgtt agacgccgtg cggctggtgt ttgtcgatgg acgttacgtg
    13741 cccgcgctga gcgatgcaat tgaaggcagc ggatatgaag tgagcattaa cgacgaccgt
    13801 caggggttac ccgacgctat tcaggcggaa gtgtttctgc atttgacgga aagcctggca
    13861 caaagcgtga cgcatatcgc cgtgaagcgc ggtcaacggc cggcaaagcc attgctgtta
    13921 atgcatatca cccagggcgt ggcaggtgaa gaggtgaaca ctgcccatta ccggcatcat
    13981 ctggatctgg cggaaggtgc cgaagcaacg gtgatcgaac attttgtcag ccttaatgat
    14041 gctcgtcatt ttaccggggc acggttcact atcaatgtcg cagcgaacgc ccacttgcag
    14101 catatcaagc tggcgtttga aaacccggtc agtcaccact ttgcccataa cgatttgttg
    14161 ctggctgacg atgccaccgc atttagccat agtttcctgc tgggtggcgc agtgttacga
    14221 cacaacacca gtacgcaact caatggcgaa aacagcacgc tgcggatcaa tagcctggcg
    14281 atgccggtga aaaacgaggt gtgtgatacc cgtacctggc tggaacacaa taaaggtttt
    14341 tgtaacagcc gacagttgca caaaactatc gtcagcgaca aaggccgcgc ggtatttaac
    14401 ggtttgatca acgtcgcgca gcacgccatc aaaacggatg gtcagatgac caacaataat
    14461 ctgctgatgg gcaaactggc ggaagtggat acaaaaccgc agctggaaat ctatgcagat
    14521 gatgtgaaat gcagccacgg cgcgacggtg gggcgtattg atgatgaaca gatgttctat
    14581 ctgcgctcgc gcgggatcaa tcagcaggat gcccagcaga tgatcattta cgcctttgct
    14641 gccgaactga cggaagcact gcgtgatgag gggcttaaac agcaggtgct ggcccgaatc
    14701 ggtcaacggc tgccaggagg tgcaagatga ctttttccgt cgacaaagtg cgggccgact
    14761 ttccggtgct ttcgcgtgag gtaaacggtt tgccgctggc ttatctcgac agcgccgcca
    14821 gtgcacagaa accgagccag gtgattgacg ccgaggccga gttttatcgt catggctacg
    14881 cggcggtgca tcgcggtatt cataccttaa gcgcccaggc gaccgagaaa atggagaacg
    14941 tgcgcaagcg ggcatcgctg tttattaatg cccgttcggc ggaagagctg gtgtttgtcc
    15001 gcggcacgac ggaagggatc aatctggtcg ccaatagctg gggcaacagc aatgtgcggg
    15061 cgggcgataa catcatcatc agtcagatgg agcaccacgc taacattgtc ccctggcaga
    15121 tgctttgcgc acgcgttggc gcagagctgc gtgtgatccc tctcaacccc gacggtacgc
    15181 tgcaactgga gacgctgcct acgctgtttg atgagaaaac tcgcctgctg gcaattactc
    15241 atgtctccaa cgtgcttggc acagaaaatc cactggcgga aatgatcacg cttgcgcacc
    15301 agcatggcgc aaaagtgctg gtggatggcg ctcaggcggt gatgcatcat ccggtggatg
    15361 ttcaggcgct ggattgcgac ttttacgtgt tctccgggca taaactgtat ggccccaccg
    15421 gaattggcat tctttatgtg aaagaagcct tgttgcagga gatgccgccg tgggaagggg
    15481 gcggttctat gatcgccacc gtcagcctga gtgaaggcac tacctggacc aaagcaccat
    15541 ggcggtttga agccggtaca cccaataccg ggggcatcat tggtcttggc gcggcgctgg
    15601 agtatgtttc ggcgctgggg cttaatagca tagccgagta tgaacagaat ctgatgcatt
    15661 atgcgctatc acagctggaa tctgtaccgg atctcactct ctatggccaa caaaacaggc
    15721 ttggcgttat tgcttttaat ctcggtaaac accacgccta tgatgttggc agttttctcg
    15781 ataattacgg cattgctgtg cgtaccggac atcactgcgc tatgccatta atggcctatt
    15841 acaacgtccc tgcgatgtgt cgggcgtcgc tggccatgta taacacccat gaagaagtgg
    15901 agcgtctggt gaccggcctg caacgtattc accgtctgct gggataacag ggaggcacta
    15961 tggctttatt gccggataaa gaaaaattgc tgcgtaattt tttacgctgc gccaactggg
    16021 aagagaaata tctctacatt attgagctgg gccagcgtct gccagaatta cgcgccgaag
    16081 acggaagccc acaaaatagc attcagggct gccagagtca ggtgtggatt gtcatgcgcc
    16141 agaatgcgca gggaattatt gaattgcagg gcgacagcga tgcggcgatt gtgaaagggc
    16201 ttattgcggt cgtctttatt ctctacgatc agatgacgcc gcaggatatt gtcaatttcg
    16261 atgtgcgtcc gtggtttgaa aaaatggcgc tcacccaaca tctcacccca tctcgttcac
    16321 aaggtctgga agcgatgatt cgcgcaattc gcgccaaagc cgctgcactt agctaaacta
    16381 aagggacagc tttcatccgg ttggctcaca ttgccggatg gtcaatgttt acactgaccg
    16441 gcacaggagt gttttggttt caggtgaaca taagcaccat ccgacgtttc agcgtgagtc
    16501 tccggcaaat acaggaggtt tacaatgaaa cgcgcgtctt tgcttacact cacgcttatc
    16561 ggcgctttta gcgccatcca ggctgcctgg gcggttgatt atccgctacc accaaccgga
    16621 agccgactgg ttgggcaaaa tcaaacgtat acggtgcaag aaggggataa aaaccttcag
    16681 gctattgccc gacgttttga tactgcagca atgttgatcc ttgaagccaa taacactatc
    16741 gccccggtgc caaaacctgg tacgacgata actattcctt cacaactgtt attacctgat
    16801 gcaccgcgtc aggggattat cgttaacctt gcagagctgc gcctttatta ttatccgccg
    16861 ggagaaaata ttgtgcaggt ttatccaata ggtattggat tgcaggggct ggaaacgccg
    16921 gtgatggaaa cgcgtgttgg gcagaaaatc cctaacccaa cctggacgcc tacggcaggc
    16981 attcgtcagc gttcgctgga gcgtggcatt aaattaccgc cagtcgttcc tgccggacca
    17041 aataacccgc taggacgtta cgcactgcgc ctcgcgcatg gtaatggcga atacctcatt
    17101 catggcacca gtgcgccgga cagcgttggt ttgcgcgtca gttcagggtg tattcgcatg
    17161 aatgcaccgg atattaaagc cttgttctcc agcgtgcgga cgggaacgcc ggtgaaagtg
    17221 atcaacgaac cggtgaaata ttccgtggaa cctaacggga tgcgttatgt tgaagtacat
    17281 cgaccactat cagcagaaga acagcaaaac gttcagacaa tgccatacac actgccagca
    17341 ggctttacgc aatttaaaga caataaggct gtagatcaga agttagtcga taaagcgttg
    17401 tatcgtcggg cagggtatcc ggtttcggtg agcagtggag caactcccgc agccagcaat
    17461 gcgccttcag tagagtcagc gcagaatggt gaaccagagc aagggaatat gttacgcgcg
    17521 acgcagtagt ggtaaatagc agacaaaaaa atggcgcaca atgtgcgcca tttttcactt
    17581 cacaggtact attacttgcg gtatttagta gccatgttgt ccagacgctg gttagcacga
    17641 gctgcgtcat ctttagcagc ctgaacgtcg gaacgcattg cgttcacgtc gttgctcagc
    17701 tggtcaactt tagcgttcag agtctgaacg tcagaagaca gctgatcgat tttagcgttg
    17761 ctggagcaac ctgccagcag agtagaaccc aggattaccg cgcccagtac cagtttagta
    17821 gctttcatta ttaataccct ctagattgag ttaatctcca tgtagcgtta caagtattac
    17881 acaaagtttt ttatgttgag aatatttttt tgatgggaag gcacttattt ttgatcgttc
    17941 gctcaaagaa gcatcgaacg ctgggtttta gataaaaaaa ttgagagaaa acaggatgct
    18001 tccatcggat tcatcttaaa taaagtgaga ttatatagtg aaatccgatt tgatttttta
    18061 ttgcttctgg ttatcgatta aataaaaaaa gcgcccatca gggcgcttcg atatacaaat
    18121 taattcacaa aagcaatatt acaggacgtg aacagatgcg gtgttagtag tgccgctcgg
    18181 taccagtgca ccagaaacca taactacgac gtcacctttg tgtgccagac cgctctgcag
    18241 agccagttct ttacccagac ggtagaaatc atcagtagaa gtgatctctt taaccagctg
    18301 cggcacaacg cctttgctca gtaccaactg atgagccgtt ttttcgttgg tggtcagtgc
    18361 caggatggtg gcatccggga agtatttacg tactgcgcga gcagatttac cgccctgggt
    18421 agcaaccacg atcagcggag catccagttt ttcagcagtt tcaacggcac cacggcatac
    18481 cgcttcggtg atgcgcagtt tacggttgtc attgttgaac tcgagacggc tgttcatcac
    18541 gcggtcggta cgttcgcaga tggtcgccat gatagaaacc gcttccagcg ggtatttacc
    18601 ttttgcggat tcaccagaca gcatcactgc gtcagtaccg tcgaggatgg cgtttgcaac
    18661 gtcaccggct tctgcgcgag tcgggcgtgg gtttttgatc atggaatcca gcatctgggt
    18721 cgcagtgata acgactttac gtgcacggat acatttttcg atcatcatct tctgggcgaa
    18781 gataacttct tctaccggga tttctacacc caggtcgcca cgcgcaacca tgatgccgtc
    18841 agaggcttcg aggatttcgt cgaagttgtt gaggccttcc tggttttcga ttttggagat
    18901 gatgtggatg ttttcgccgc cgtgcgcttt caggtgctca cggatttcga taacgtcaga
    18961 acgcttacga ataaaggaag cagcaacaaa gtctacgcct ttttcgcaac caaagatcag
    19021 gtcctgtttg tctttttcag ccagtgctgg cagagcaatg gaaacgccag gcaggttcac
    19081 acctttgttt tcgcccaggt caccgttgtt cagcacttta cagataactt tgttaccttc
    19141 aatggcggta acttccatac cgatcagacc atcgtcaacc agtacggtgt tgccaacaga
    19201 caggtcagta gtgaaacctt catacgttac cgcaaccatt tcgctgttgc cgataacaga
    19261 tttatcagtg gtgaaagtaa aggtctgacc agctttcaga gaaacatcgt taccgccttc
    19321 cagtttcatg gtgcggattt ccggaccttt ggtatcaagc aggatagcgg cggttttacc
    19381 agttttgctc atcacgttgc gcagattctg aatgcgctga ccgtgttctg catagtcacc
    19441 atgagagaag ttcagacgca taacgttcat gccagcgtcc agcattttag ctaacatctc
    19501 ttcagattcg gttttcggtc cgatggtgca aacaattttg gtctttttca tgacagtctt
    19561 agtctttaag ttgagaagga tgggagaaac ttgctttctg ggcgcactgc cggaaagcga
    19621 atccggtatt acgaaagtgg tggtgcctcg ctctaaggat aggtgacatc gaaaaagcgt
    19681 gcagaggaat gtgtgcttgt gtctcagccc aacgtgagtg tctgtttttg agtcgttgag
    19741 ttctacagtc caaagtgctg aaaccattca agagtcaatt ggcgcgcatt atacgctgaa
    19801 attaatccaa aaggaaaaca aaaacagcgt ttcaaaaaat atacaacttg ttagacaata
    19861 aattgttatc aaagatttaa cgttccaggg aaaaggttac gcaaaaactt cgctgtttgc
    19921 gcaacgtctg gtgccgaagt tgtcagtgtg aggataagtg aaagttcatc cggtgtataa
    19981 atatatcatc gctcaattgc tacagttaaa ataatttatc gcgttatttg tctgattttg
    20041 tttgattttt gtttaattgg ttagattgat atatgctgat
//
LOCUS       DAJTRC010000073        19598 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.073, whole genome shotgun sequence.
ACCESSION   DAJTRC010000073 DAJTRC010000000
VERSION     DAJTRC010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.073"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            208..1305
                     /gene="mltA"
                     /locus_tag="OO357_002789"
     CDS             208..1305
                     /gene="mltA"
                     /locus_tag="OO357_002789"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008785702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase A"
                     /protein_id="HCR5887677.1"
                     /translation="MKGRWVKYLLMGTVVAMLAACSSKPTDRGQQYKDGKFTQPFSLV
                     NQPDAVGAPINAGDFAEQINHIRNSSPRLYGNQSNVYNAVQEWLRAGGDTRNMRQFGI
                     DAWQMEGADNYGNVQFTGYYTPVIQARHTRQGEFQYPIYRMPPKRGRLPSRAEIYAGA
                     LGDKYILAYSNSLMDNFIMDVQGSGYIDFGDGSPLNFFSYAGKNGHAYRSIGKVLIDR
                     GEVKKEDMSMQAIRHWGETHSEAEVRELLEQNPSFVFFKPQSFAPVKGASAVPLVGRA
                     SVASDRSIIPPGTTLLAEVPLLDNNGKFNGQYELRLMVALDVGGAIKGQHFDIYQGIG
                     PEAGHRAGWYNHYGRVWVLKTAPGAGNVFSG"
     gene            1543..2349
                     /gene="tcdA"
                     /locus_tag="OO357_002790"
     CDS             1543..2349
                     /gene="tcdA"
                     /locus_tag="OO357_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA cyclic N6-threonylcarbamoyladenosine(37)
                     synthase TcdA"
                     /protein_id="HCR5887678.1"
                     /translation="MSVVISDAWRQRFGGTARLYGEKALQLFADAHICVVGIGGVGSW
                     AAEALARTGIGAITLIDMDDVCVTNTNRQIHALRDNVGLAKAEVMAERIRQINPECRV
                     TVVDDFVTPDNVAQYMSAGYSYVIDAIDSVRPKAALIAYCRRNKIPLVTTGGAGGQID
                     PTQIQVTDLAKTIQDPLAAKLRERLKSDFGVVKNSKGKLGVDCVFSTEALVYPQSDGT
                     VCAMKATAEGPKRMDCASGFGAATMVTATFGFVAVSHALKKMMAKAARQG"
     gene            complement(2400..2843)
                     /gene="csdE"
                     /locus_tag="OO357_002791"
     CDS             complement(2400..2843)
                     /gene="csdE"
                     /locus_tag="OO357_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase sulfur acceptor subunit
                     CsdE"
                     /protein_id="HCR5887679.1"
                     /translation="MTNPQFAGHPFGTTVTAETLRNTFAPLSQWEDKYRQLIMLGKQL
                     PALPDELKAQAKEIAGCENRVWLGYTVAENGKMHFFGNSEGRIVRGLLAVLLTAVEGK
                     TAAELQAQSPLALFDELGLRAQLSASRSQGLNALSEAIIAATKQV"
     gene            complement(2843..4048)
                     /gene="csdA"
                     /locus_tag="OO357_002792"
     CDS             complement(2843..4048)
                     /gene="csdA"
                     /locus_tag="OO357_002792"
                     /EC_number="3.13.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase CsdA"
                     /protein_id="HCR5887680.1"
                     /translation="MNVFNPAQFRAQFPALQDAGVYLDSAATALKPEAVVEATRQFYS
                     LSAGNVHRSQFAEAQRLTARYEAAREKVAQLLNAPDDKTIVWTRGTTESINMVAQCYA
                     RPRLQPGDEIIVSVAEHHANLVPWLMVAQQTRAKVVKLPLNAQRLPDVDLLPELITPR
                     SRILALGQMSNVTGGCPDLARAITFAHSAGMVVMVDGAQGTVHFPADVQQLDIDFYAF
                     SGHKLYGPTGIGVLYGKSELLEAMSPWLGGGKMIHEVSFDGFTTQSAPWKLEAGTPNV
                     AGVIGLSAALEWLADYDINQAESWSRSLATLAEEALAKRPGFRSFRCQDSSLLAFDFA
                     GVHHSDMVTLLAEYGIALRAGQHCAQPLLAELGVTGTLRASFAPYNTKSDVDALVNAV
                     DRALELLVD"
     gene            4240..4467
                     /locus_tag="OO357_002793"
     CDS             4240..4467
                     /locus_tag="OO357_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgdI/YgdR family lipoprotein"
                     /protein_id="HCR5887681.1"
                     /translation="MKKTAAIISACMLTFALSACSGSNYVMHTNDGRTIVSDGKPQTD
                     NDIGMISYKDANGNKQQINRTDVKEMVELEQ"
     gene            4818..5735
                     /gene="gcvA"
                     /locus_tag="OO357_002794"
     CDS             4818..5735
                     /gene="gcvA"
                     /locus_tag="OO357_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system transcriptional
                     regulator GcvA"
                     /protein_id="HCR5887682.1"
                     /translation="MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSL
                     EDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLP
                     SFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
                     YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGFNHINVQQGP
                     IFSHSAMVLQAAIHGQGVALANNVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQA
                     ELGKIAAFRQWILAKAAAEQEKFRFRYEQ"
     gene            5754..6149
                     /locus_tag="OO357_002795"
     CDS             5754..6149
                     /locus_tag="OO357_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HCR5887683.1"
                     /translation="MTSRFMLIFAAISGFIFVALGAFGAHVLSKTMGAVEMGWIQTGL
                     EYQAFHTLAILGLAVAMQRRISIWFYWSSVFLALGTVLFSGSLYCLALSHLRLWAFVT
                     PVGGVSFLAGWALMLVGAIRLKRKGVSHE"
     gene            6142..7242
                     /gene="rlmM"
                     /locus_tag="OO357_002796"
     CDS             6142..7242
                     /gene="rlmM"
                     /locus_tag="OO357_002796"
                     /EC_number="2.1.1.186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010435097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (cytidine(2498)-2'-O)-methyltransferase
                     RlmM"
                     /protein_id="HCR5887684.1"
                     /translation="MNKVVLLCRPGFEKECAAEITDKAGQREIFGFARVKENAGYVIY
                     ECYQPDDGDKLIRELPFSSLIFARQWFVVGELLQHLPPEDRITPIVGMLQGVVEKGGE
                     LRVEVADTNESKELLKFCRKFTVPLRAALRDAGVLANYETPKRPVVHVFFIAPGCCYT
                     GYSYSNNNSPFYMGIPRLKFPAEAPSRSTLKLEEAFHVFIPADEWDERLANGMWAVDL
                     GACPGGWTYQLVKRNMWVYSVDNGPMAQSLMDTGQVTWLREDGFKFRPTRSNISWMVC
                     DMVEKPAKVAALMAQWLVNGWCRETIFNLKLPMKKRYEEVSHNLAYIQAQLDEHGINA
                     QIQARQLYHDREEVTVHVRRIWAAVGGRRDER"
     gene            complement(7286..8017)
                     /gene="fucR"
                     /locus_tag="OO357_002797"
     CDS             complement(7286..8017)
                     /gene="fucR"
                     /locus_tag="OO357_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose operon activator"
                     /protein_id="HCR5887685.1"
                     /translation="MKAARQQAIVDLLLNHTSLTTEALSEQLKVSKETIRRDLNELQT
                     QGKILRNHGRAKYIHRQNQDSGDPFHIRLKSHYAHKADIAREALAWIEEGMVIALDAS
                     STCWYLARQLPDINIQVFTNSHPICHELGKRERIQLISSGGTLERKYGCYVNPSLISQ
                     LKSLEIDLFIFSCEGIDSSGALWDSNAINADYKSMLLKRAAQSLLLIDKSKFNRSGEA
                     RIGHLDEVTHIISDERQVATSLVTA"
     gene            complement(8075..8497)
                     /gene="fucU"
                     /locus_tag="OO357_002798"
     CDS             complement(8075..8497)
                     /gene="fucU"
                     /locus_tag="OO357_002798"
                     /EC_number="5.1.3.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose mutarotase"
                     /protein_id="HCR5887686.1"
                     /translation="MLKTISPLISPELLKVLAEMGHGDEIIFSDAHFPAHSMEPQVIR
                     ADGLLVSDLLQAIIPLFELDSYAPPLVMMAAVEGDTLDPEVERRYRNALSLQAPCPDI
                     IRINRFAFYERAQKAFAIVITGERAKYGNILLKKGVTP"
     gene            complement(8499..9918)
                     /gene="fucK"
                     /locus_tag="OO357_002799"
                     /pseudo
     CDS             complement(8499..9918)
                     /gene="fucK"
                     /locus_tag="OO357_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417283.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculokinase"
     gene            complement(10027..11802)
                     /gene="fucI"
                     /locus_tag="OO357_002800"
     CDS             complement(10027..11802)
                     /gene="fucI"
                     /locus_tag="OO357_002800"
                     /EC_number="5.3.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose isomerase"
                     /protein_id="HCR5887687.1"
                     /translation="MKKISLPKIGIRPVIDGRRMGVRESLEEQTMNMAKATAALLTEK
                     LRHACGAAVECVISDTCIAGMAEAAACEEKFSSQNVGLTITVTPCWCYGSETIDMDPT
                     RPKAIWGFNGTERPGAVYLAAALAAHSQKGIPAFSIYGHDVQDADDTSIPADVEEKLL
                     RFARAGLAVASMKGKSYLSLGGVSMGIAGSIVDHNFFESWLGMKVQAVDMTELRRRID
                     QKIYDEAELEMALAWADKNFRYGEDENNKQYQRNAEQSRAVLRESLLMAMCIRDMMQG
                     NSKLADIGRVEESLGYNAIAAGFQGQRHWTDQYPNGDTAEAILNSSFDWNGVREPFVV
                     ATENDSLNGVAMLMGHQLTGTAQVFADVRTYWSPEAIERVTGHKLDGLAEHGIIHLIN
                     SGSAALDGSCKQRDSEGNPTMKPHWEISQQEADACLAATEWCPAIHEYFRGGGYSSRF
                     LTEGGVPFTMTRVNIIKGLGPVLQIAEGWSVELPKDVHDILNKRTNSTWPTTWFAPRL
                     TGKGPFTDVYSVMANWGANHGVLTIGHVGADFITLASMLRIPVCMHNVEETKVYRPSA
                     WAAHGMDIEGQDYRACQNYGPLYKR"
     gene            complement(11835..13151)
                     /gene="fucP"
                     /locus_tag="OO357_002801"
     CDS             complement(11835..13151)
                     /gene="fucP"
                     /locus_tag="OO357_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fucose:H+ symporter permease"
                     /protein_id="HCR5887688.1"
                     /translation="MGNTSIQTQSYRAVDKDAGQSRSYIIPFALLCSLFFLWAVANNL
                     NDILLPQFQQAFTLTNFQAGLIQSAFYFGYFIIPIPAGILMKKLSYKAGIITGLFLYA
                     LGAALFWPAAEIMNYTLFLVGLFIIAAGLGCLETAANPFVTVLGPESSSHFRLNLAQT
                     FNSFGAIIAVVFGQSLILSNVPHQSQDVLDKMSPEQLSAYKHSLVLSVQTPYMIIVAI
                     VLLVALLIMLTKFPALQSDNHSDAKQGSFSASLSRLARIRHWRWAVLAQFCYVGAQTA
                     CWSYLIRYAVEEILGMTAGFAANYLTGTMVCFFIGRFTGTWLISRFAPHKVLAAYALI
                     AMALCLISAFAGGHVGLIALTLCSAFMSIQYPTIFSLGIKNLGQDTKYGSSFIVMTII
                     GGGIVTPVMGFVSDAAGNIPTAELIPALCFAVIFIFARFRSQTATN"
     gene            13698..14345
                     /gene="fucA"
                     /locus_tag="OO357_002802"
     CDS             13698..14345
                     /gene="fucA"
                     /locus_tag="OO357_002802"
                     /EC_number="4.1.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuculose-phosphate aldolase"
                     /protein_id="HCR5887689.1"
                     /translation="MERNKLARQIIDTCLEMTRLGLNQGTAGNVSVRYQDGMLITPTG
                     IPYEKLTESHIVFIDGNGKHEEGKLPSSEWRFHMAAYQSRPDANAVVHNHAVHCTAVS
                     ILNRSIPAIHYMIAAAGGNSIPCAPYATFGTRELSEHVALALKNRKATLLQHHGLIAC
                     EVNLEKALWLAHEVEVLAQLYLTTLAITDPVPVLSDEEIAVVLEKFKTYGLRIEE"
     gene            14373..15521
                     /gene="fucO"
                     /locus_tag="OO357_002803"
     CDS             14373..15521
                     /gene="fucO"
                     /locus_tag="OO357_002803"
                     /EC_number="1.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708594.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactaldehyde reductase"
                     /protein_id="HCR5887690.1"
                     /translation="MANRMILNETAWFGRGAVGALTDEVKRRGYQKALIVTDKTLVQC
                     GVVAKVTDKMDAAGLAWAIYDGVVPNPTITVVKEGLGVFQNSGADYLIAIGGGSPQDT
                     CKAIGIISNNPEFADVRSLEGLSPTNKPSVPILAIPTTAGTAAEVTINYVITDEEKRR
                     KFVCVDPHDIPQVAFIDADMMDGMPPALKAATGVDALTHAIEGYITRGAWALTDALHI
                     KAIEIIAGALRGSVAGDKDAGEEMALGQYVAGMGFSNVGLGLVHGMAHPLGAFYNTPH
                     GVANAILLPHVMRYNADFTGEKYRDIARVMGVKVEGMSLEGARNAAVEAVFALNRDVG
                     IPPHLRDVGVRKEDISALAQAALDDVCTGGNPREATLEDIVELYHTAW"
     gene            complement(15574..16329)
                     /gene="xni"
                     /locus_tag="OO357_002804"
     CDS             complement(15574..16329)
                     /gene="xni"
                     /locus_tag="OO357_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417278.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flap endonuclease Xni"
                     /protein_id="HCR5887691.1"
                     /translation="MAVHLLIVDALNLIRRIHAVQGSPCVETCQHALDQLIMHSQPTH
                     AVAVFDDENRSSGWRHQRLPDYKADRPPMPEELHDEMPALRAAFEQRGVPCWSASGNE
                     ADDLAATLAVKVTQAGHQATIVSTDKGYCQLLSPTLRIRDYFQKRWLDAPFIDKEFGV
                     QPQQLPDYWGLAGISSSKVPGVAGIGPKSATQLLVEFQSLEGIYENLDAVAEKWRKKL
                     ETHKEMAFLCRDIARLQTDLHIDGNLQQLRLVR"
     gene            complement(16440..17807)
                     /gene="sdaB"
                     /locus_tag="OO357_002805"
     CDS             complement(16440..17807)
                     /gene="sdaB"
                     /locus_tag="OO357_002805"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase II"
                     /protein_id="HCR5887692.1"
                     /translation="MISVFDIFKIGIGPSSSHTVGPMKAGKQFTDDLIARNLLKDVTR
                     VVVDVYGSLSLTGKGHHTDIAIIMGLAGNLPDTVDIDSIPGFIQDVNTHGRLMLANGQ
                     HEVEFPVDQCMNFHADNLSLHENGMRITALAGDKVVYSQTYYSIGGGFIVDEEHFGQQ
                     DSAPVEVPYPYSSAADLQKHCQETGLSLSGLMMKNELALHSKEELEQHLANVWEVMRG
                     GIERGISTEGVLPGKLRVPRRAAALRRMLVSQDKTTTDPMAVVDWINMFALAVNEENA
                     AGGRVVTAPTNGACGIIPAVLAYYDKFIREVNANSLARYLLVASAIGSLYKMNASISG
                     AEVGCQGEVGVACSMAAAGLAELLGASPAQVCIAAEIAMEHNLGLTCDPVAGQVQVPC
                     IERNAIAAVKAVNAARMALRRTSEPRVCLDKVIETMYETGKDMNAKYRETSRGGLAMK
                     IVACD"
     gene            complement(17865..19154)
                     /gene="sdaC"
                     /locus_tag="OO357_002806"
     CDS             complement(17865..19154)
                     /gene="sdaC"
                     /locus_tag="OO357_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAAAP family serine/threonine permease SdaC"
                     /protein_id="HCR5887693.1"
                     /translation="METTQTSTIASKDSRSAWRKTDTMWMLGLYGTAIGAGVLFLPIN
                     AGVGGMIPLIIMAILAFPMTFFAHRGLTRFVLSGKNPGEDITEVVEEHFGIGAGKLIT
                     LLYFFAIYPILLVYSVAITNTVESFMSHQLGMTPPPRAILSLILIVGMMTIVRFGEQM
                     IVKAMSILVFPFVGVLMLLALYLIPQWNGAALETLSLDTASATGNGLWMTLWLAIPVM
                     VFSFNHSPIISSFAVAKREEYGDMAEQKCSKILAFAHIMMVLTVMFFVFSCVLSLTPA
                     DLAAAKEQNISILSYLANHFNAPVIAWMAPIIAIIAITKSFLGHYLGAREGFNGMVIK
                     SLRGKGKSIEINKLNRITALFMLVTTWIVATLNPSILGMIETLGGPIIAMILFLMPMY
                     AIQKVPAMRKYSGHISNVFVVVMGLIAISAIFYSLFS"
BASE COUNT         4825 a         4806 c         5172 g         4795 t
ORIGIN      
        1 ccgtggatgc gcactatact ccgctcgcgt tttcatgcaa cctttttttt accccgatca
       61 cggatttagc atgattttga acaaaaacaa accactaaag cctatttttt gtgcaaaatt
      121 tgcgtcaggg agaggctgct catttcatta aacgcggcgg tttgttatct tcgttgcgcc
      181 ttatttttta acctgaagaa gagaacaatg aaaggacgtt gggtaaagta ccttcttatg
      241 ggcacggttg tggcaatgct tgccgcctgc tcttccaaac caaccgatcg cggacagcaa
      301 tataaagacg ggaaatttac ccagcctttc tctctggtga accagccaga tgccgttggc
      361 gcgccgatta acgccggtga ttttgccgag caaattaacc atatccgtaa ttcgtcaccg
      421 cgtctgtatg gcaaccagag taatgtttat aacgcggtgc aagagtggct gcgcgcaggc
      481 ggtgataccc gcaatatgcg ccagttcggc attgatgcct ggcagatgga aggtgccgac
      541 aactatggta acgtgcagtt tacaggctat tacacgccgg taattcaggc gcgccatacc
      601 cgccagggcg agttccagta tcctatttac cgtatgccgc caaaacgtgg tcgtctgccg
      661 tctcgtgcgg agatctacgc cggggcattg ggcgataaat acatcctcgc ttacagcaac
      721 tctctgatgg ataacttcat tatggatgtg cagggtagtg ggtatatcga ctttggtgat
      781 ggcagtccgc ttaacttttt cagctatgca gggaaaaacg gtcatgccta tcgcagcatt
      841 ggtaaggtgc tgatcgaccg tggcgaagtg aaaaaagaag atatgtcgat gcaggcgatt
      901 cgtcattggg gcgaaacaca cagtgaagcc gaggttcgcg agctgctgga acagaacccg
      961 tctttcgtct tctttaaacc gcaatctttt gcaccggtga aaggggcaag tgcggtgccg
     1021 ctggttggcc gtgcttctgt tgcctccgat cgttcaatta ttccgccagg tactaccttg
     1081 ctggcagaag tgccgctgct ggataataac ggcaaattta acggtcagta cgaattgcgt
     1141 ctgatggtgg cgttggatgt cgggggcgca atcaaaggcc aacacttcga tatctatcaa
     1201 gggatcgggc cggaagccgg acaccgcgca ggttggtaca accactatgg acgggtttgg
     1261 gtgctgaaaa cggctccggg cgcaggtaac gtctttagcg gctgatgtga tattctgtat
     1321 gccgttttta cggatatgac aggggagggg taacctcaca tcctgattat tgactaagtg
     1381 cgcgttgttc ataccggatg cggcgtgaac gccttatccg gcctacaaat catgcaaatc
     1441 caaaatattg caggagttat gtaggcctga taagcgtagc gcatcaggca tttatgcgtt
     1501 tagggtgagg gataacctcg ccctttttaa ttctgaggtg ctatgtctgt ggtaattagt
     1561 gatgcatggc gtcagcgttt tggtggcaca gcgcgtctgt atggtgaaaa agcgttgcaa
     1621 ctgtttgctg acgcgcatat ttgtgtggtt ggtatcggtg gtgtcggttc ctgggcggcg
     1681 gaagcgctgg cgcgcacggg gattggcgca atcacgctta tcgatatgga tgatgtgtgc
     1741 gtcaccaata ccaatcggca aattcacgct ctgcgcgata acgtcgggct ggcaaaagcg
     1801 gaagtcatgg cggagcgcat tcgccagatt aacccggagt gccgtgtaac ggtggtggat
     1861 gatttcgtga cgccggataa cgtagcgcaa tatatgagtg ccggttattc gtacgtgatt
     1921 gatgccattg atagtgtacg gcccaaagcg gcgctgattg cttattgtcg gcgcaataaa
     1981 atcccgctgg tcacgaccgg tggcgcgggt gggcagattg acccgacgca gattcaggtt
     2041 accgatctgg cgaaaacgat tcaggatccg ctggcggcga agttgcgcga gcgcctgaaa
     2101 agcgattttg gcgtagtgaa aaacagtaaa ggtaagctcg gcgtggattg cgtgttttct
     2161 actgaagcgc tggtgtaccc gcagtcagac ggtacggtgt gtgcgatgaa agccacggca
     2221 gaagggccga agcggatgga ttgtgcatct ggatttggcg cggcaacgat ggtgaccgcc
     2281 acctttgggt ttgttgcggt ttctcatgcg ctgaagaaga tgatggcgaa agcggcgcgt
     2341 cagggttaaa caatcaggtc ggatgagacg cgaacgtctt atccgacccg gttctgtgct
     2401 taaacctgct tcgtcgcagc gataatcgcc tcgcttaacg catttaaccc ctggctgcgt
     2461 gaggcgctaa gctgcgcacg taatcccagc tcatcaaaca atgccagcgg cgactgtgcc
     2521 tgtaattcgg cggcggtttt cccctcaacg gcagtcaaca acaccgccaa caggccgcgc
     2581 acaatgcgcc cttcgctgtt gccaaagaag tgcattttgc cgttttcagc cacggtatat
     2641 cccagccaga cgcggttttc gcatccggca atctctttag cctgcgcttt taactcgtct
     2701 ggcaaagccg gaagctgttt ccccagcatg atcaactggc gatatttatc ttcccattgc
     2761 gacaacggtg cgaaggtatt gcgtaacgtt tctgcggtta cggttgtgcc gaacggatgt
     2821 ccggcgaatt gcgggtttgt cattaatcca ccaataattc cagcgcgcgg tcaacggcat
     2881 tcaccagcgc atccacatca ctctttgtat tatatggcgc aaatgaggcg cgcagtgtgc
     2941 cggttacgcc taattctgcc agtagcggct gagcgcaatg ttgcccggcc cgcaaggcaa
     3001 taccgtactc cgccagtagc gtcaccatat cgctgtgatg aacgccagca aaatcaaagg
     3061 ccagcaggct ggaatcctgg cagcggaatg aacgaaagcc tggacgtttc gccagcgctt
     3121 cttctgcaag cgttgctaag ctacggctcc agctttcggc ctggttgata tcgtaatctg
     3181 ccagccattc cagcgccgcg cttaatccta tgacgccagc gacatttggc gttcctgctt
     3241 ccagtttcca cggcgcagat tgagtggtga aaccgtcaaa actcacttcg tgaatcattt
     3301 tgccgccacc cagccagggc gacatcgctt ccagcagttc tgatttgcca tacagcacgc
     3361 cgatacccgt cgggccatac agtttgtgac cagagaaagc atagaaatcg atatccagtt
     3421 gctgaacatc cgcggggaaa tgcaccgtcc cctgagcacc atcaaccatc accaccatcc
     3481 cggctgaatg agcaaaggta atcgctcgcg ccagatccgg gcaaccgcca gtcacgttcg
     3541 acatctgacc caacgccaga atccgactac ggggagtaat cagctctggc aacaaatcga
     3601 catccggcag tcgctgcgca ttaagcggca atttcaccac tttggctcta gtttgttggg
     3661 cgaccatcag ccaggggacg aggttagcgt ggtgttctgc cacgctgaca ataatctcat
     3721 cgcccggttg cagacgcgga cgcgcatagc attgtgccac catgttgatg gattcagtgg
     3781 tgccgcgcgt ccagacgata gttttatcat ccggtgcatt cagtaattgc gccactttct
     3841 ctcgtgcagc ttcataacgc gcggtcaggc gttgggcttc ggcaaactgg ctgcgatgga
     3901 cgtttccggc gctcaggctg taaaactgtc gagtggcttc aaccacggct tcaggtttaa
     3961 gcgcggtcgc ggcgctgtcg agatagacgc ccgcatcctg tagtgcggga aactgggcgc
     4021 gaaactgcgc gggattaaaa acgttcatgg tactcctcgg cttgatgcac cgatcgtgac
     4081 gcaaaattca ctttgataca aggagagtgc taaccatcag aattatctgg atatcctggc
     4141 gaagccgcgt aagtaacata tgctgaatag tgttaggcgt taattttagc ctgttaatca
     4201 atactattaa gatttattaa tcgttttaag gagaagatga tgaaaaagac tgccgcaatt
     4261 atttctgcct gtatgctgac ttttgccctg agcgcctgtt ccggttcgaa ctatgtgatg
     4321 cacaccaatg acggacgtac catcgtctct gacggcaaac cacagactga taacgatatc
     4381 ggtatgattt cgtataaaga cgctaatggc aacaaacagc agatcaaccg tactgacgtg
     4441 aaagagatgg tcgaactgga acagtaattc gcgatcgcaa ggtaaaaaaa agcaccgcaa
     4501 ttaggcggtg ctacattaat cactatggac agacagggta aatgtacagg aagtgaaaaa
     4561 aggtagcttt gctaccatgg tctgaatcgc agaccaattg caaacacaac aacacaacat
     4621 cacaaccgta agccaaaagt tcaccagaat acgcattccg ataaaacttt tcgttccggc
     4681 tcaggaagtg ccgccactat aggtatttgc tggtagaagc tcaacggaca atttataatg
     4741 gctcagatta aaaaaactaa taggttacac agtgtgatct aattgttaaa ttcatttaac
     4801 atcaaagttt aaaagccatg tctaaacgat taccaccgct aaatgcctta cgagtttttg
     4861 atgccgcagc acgccattta agttttactc gcgcagcaga agagcttttt gtgacccagg
     4921 ccgcagtaag tcatcaaatc aagtctcttg aggatttttt ggggctaaaa ctgttccgcc
     4981 gccgtaatcg ttcactcctg ctgaccgagg aagggcaaag ctatttcctc gatatcaaag
     5041 agatattttc gcaattaacc gaagcgacgc gtaaactcca ggcccgtagc gccaaggggg
     5101 cgttgacggt cagtttactc cccagtttcg ccattcattg gttggttccg cgactttcca
     5161 gctttaattc agcttatccg ggaattgacg ttcgaatcca ggcggttgat cgtcaggaag
     5221 ataagctggc ggatgatgtt gatgtggcga tattttatgg tcggggcaac tggccggggc
     5281 tacgggtgga aaaactgtac gccgaatatt tattgccggt gtgttcgccg ctactgctga
     5341 caggcgaaaa acccttgaag accccggaag atctggctaa acatacgtta ttacatgatg
     5401 cgtcacgccg tgactggcag acatataccc gacagttggg gtttaatcat atcaacgttc
     5461 agcaagggcc aatttttagt catagcgcca tggtgctgca agcggctatt cacgggcagg
     5521 gagtggcgct ggcaaataac gtgatggcgc aatctgaaat cgaggccgga cgtcttgttt
     5581 gcccgtttaa tgatgttctg gtcagtaaaa atgcttttta tctggtttgt catgacagcc
     5641 aggcagaact gggtaaaata gccgcctttc gccaatggat cctggcgaaa gccgctgctg
     5701 aacaagaaaa attccgcttt cgttatgaac aataatttac gtagggtacg accatgacca
     5761 gccgttttat gctgattttc gccgccatta gcggcttcat ttttgtggca ctgggcgctt
     5821 ttggtgcgca tgtgttaagt aaaaccatgg gggccgttga gatgggctgg atccaaaccg
     5881 gtctcgaata ccaggcgttt catacgctgg cgatcttagg tctggcggtg gcaatgcagc
     5941 gtcgcatcag tatctggttt tactggagta gcgttttcct cgcgttaggc acggtgctgt
     6001 tcagcggcag cctttattgc ctggcgctgt cccatctgcg tttgtgggcg tttgtcacac
     6061 cggttggcgg cgtgagcttc cttgcgggct gggcgttaat gttagttggt gctatccgtt
     6121 taaagcgcaa gggcgtaagt catgaataag gttgtattgc tgtgccgtcc gggctttgaa
     6181 aaagagtgcg ccgcagaaat taccgataaa gccggccagc gggaaatttt cggttttgcc
     6241 cgcgtgaaag agaatgcggg ttatgtcatt tatgaatgtt atcaacctga tgatggcgat
     6301 aagttaatcc gtgagctgcc gttcagttca ttaatttttg cccgccagtg gtttgtggtg
     6361 ggggaactcc tgcagcattt gccgccagaa gatcgtatta cccccattgt cggcatgtta
     6421 cagggtgtag tagagaaggg cggtgaactg cgtgttgaag ttgccgatac caacgaaagc
     6481 aaagagttgc tgaaattctg ccgtaagttc actgttccgc tacgcgctgc cttgcgcgat
     6541 gctggggtgc tggcgaacta tgaaacgccg aagcgtccgg ttgtgcatgt attctttatt
     6601 gcaccaggat gctgctatac cggttactca tacagcaaca ataattcgcc gttctatatg
     6661 ggcattccgc gcctgaaatt tccggcagag gcgccgagtc gttccacgct caaactggaa
     6721 gaggcatttc atgtgtttat tcctgcggat gagtgggatg aacgcctggc gaacgggatg
     6781 tgggcggtgg atttaggcgc ttgccctggc ggctggacct accaactggt gaagcgcaac
     6841 atgtgggttt attccgtcga caacggcccg atggcgcaaa gtctgatgga taccgggcag
     6901 gtgacgtggc tgcgggaaga cggtttcaaa ttccgtccga cacgcagcaa tatctcctgg
     6961 atggtatgcg atatggttga aaaaccggct aaagttgcgg cattgatggc gcagtggctg
     7021 gttaatggct ggtgccgtga aaccattttc aatctcaaac tgccgatgaa aaaacgctac
     7081 gaagaagtat cacacaatct ggcgtatatt caggcacagc ttgatgaaca tggcataaat
     7141 gctcagattc aggcgcggca gttgtatcac gatcgcgaag aagtgacggt gcacgtccgc
     7201 cgcatctggg ctgcggtggg tggtcgtcgc gacgagcgat aacaggcaag gccggggcgt
     7261 cgtctccggc ctgctaccct ttccgtcagg ctgttaccaa agaagttgca acctggcgct
     7321 catcagaaat aatgtgcgtt acctcatcca gatgcccgat gcgggcttcc cctgaacgat
     7381 taaatttact tttatcaatc aataacaacg attgcgcggc acgttttaat agcatcgatt
     7441 tgtaatcagc gttgatcgca ttggagtccc acagtgcgcc gctgctatcg atcccttcac
     7501 aagaaaaaat aaacagatcg atttccagcg atttaagttg ggaaatcagc gagggattga
     7561 cgtaacagcc atatttgcgc tcaagtgtgc cgccggaact gatcagttga atgcgttcgc
     7621 gtttaccgag ttcatggcaa atcggatggc tattggtgaa gacctgaatg ttgatgtcag
     7681 gcaactggcg tgccagatac cagcaagttg aactggcgtc taaggctatc accatccctt
     7741 cttcaatcca cgcgagcgcc tcgcgcgcga tatctgcttt atgcgcataa tggcttttca
     7801 gcctgatgtg aaagggatcg ccactgtctt gattttgacg gtggatatat ttagcgcgtc
     7861 catgattgcg cagaatttta ccctgcgtct gtaattcatt gagatcgcga cgaatggttt
     7921 ctttactgac ctttagctgt tcagagagag cttccgtggt caggctggta tggttcagca
     7981 gcaggtcgac tatcgcttgc tggcgtgccg ctttcatatt tttcacccca tcattcgata
     8041 accgcccgcg tcatgcgggc gtaccggtat gagattacgg tgttacccct ttttttaaaa
     8101 gaatattccc gtacttcgct cgttcgcctg tgataacgat cgcaaaggct ttttgcgccc
     8161 gttcataaaa cgcaaaacga ttgatgcgga tgatgtcagg acacggggct tgtagtgaaa
     8221 gcgcattacg gtaacgtcgt tctacttcag gatcgagagt gtcaccctct accgccgcca
     8281 tcatcaccag cggcggggca taactgtcca gctcaaataa cgggataatc gcctggagca
     8341 agtcgctcac caacaggcca tcagcgcgga tcacctgcgg ttccatcgaa tgggcgggaa
     8401 agtgagcatc ggaaaaaata atttcatctc catgtcccat ctctgccagc actttcaata
     8461 gttcgggaga aattaacggc gaaattgttt tcagcatttc acacttcctc tataaattca
     8521 ggttcagttt gcgggtagaa ataacggtac tgataatgaa tctgtgcacg ggcttcttcc
     8581 gggctgttaa attcccctac gccataccaa ccgaacagcg cagctcctgc gacggtcgtt
     8641 tcggcatcgt cgagaacttt taccggaata tcaagcatat tggctttaat ctgattccac
     8701 aatgtgttgc gacttcctcc accgactaac aataattcat aggctttaaa gtgcccgatt
     8761 ttttccagca tctgtagatt gcgctgtaat tgcgcagtta acccttccag cgccgcgcga
     8821 tagaaatgcc cccgcgtggt attaagcgtc actccttgcc agccagcgtt ctgacacgac
     8881 aataaatcac actgcatttt cacaccatcc gcgccaggtg cgatcagacg agcttcttca
     8941 atcaacattt gccagggtgt ttcagccgtc cagaacagtt ttctcaccca ttccagcacg
     9001 ccggatgcca gccattgcat acctgggtta tacaaccatg cctggctatc tagttcgcag
     9061 gtggaaccgg cgtactgact taacagcgaa gtatcaacct aggcgctgcg aaccattaaa
     9121 atttcccatg taccggagga gagcacgggt tcattttgtt cggcaccagc gccaaaaagg
     9181 gcgaactggg tatcgtgacc tgcggaaatc accggtatgc caacgggtaa gccgagcatt
     9241 gctgcggcgt tgttctgtag cgtaccaatc tgttcacccg cttccaccag acgagggaag
     9301 agtcggcgtg gaataccggt ggcttgtaaa atttgcggac tgaaatcgcg ttgctggata
     9361 tccagcatct ggctggttcc ggccatcgtg atatcagtag tgaattcgct ggttaaacgg
     9421 tggttaatca gcgacgaaat gaagagccag gcgtgcgcgc gttccagcag ttgtggatga
     9481 ttttctttca accacaccaa cttatataac gtattgaaac taaaggctcc gacgccagaa
     9541 atagcctgca accgctgtgc ggagattaac cgttcaatat tgtccataac cgctgctgtt
     9601 cgcggacatt tccagctaat gatcggatag agcagattgc cttgcttatc taccagagcg
     9661 ccatccacac taaaggtggt gacggcgata ccgcggatgt ggcattcagt cagttcacta
     9721 ttgatttgcc gacagcaatc agcaaagcgt tgcaaaatgg cagtctaaag accactggtg
     9781 ccaggtgttg ttttccatcg cgatatcgct ggcattaggc gttgaggcgc gggcaacaat
     9841 ttttccctgc cgattaaccg cgatggccct gacattggtc gcgccacagt cgagtaccag
     9901 gataacttct tgtttcataa tcgctcctgc aatatagccg gataacattg tttatccggc
     9961 taaccactct tgcttgttca ggcccggtag ccggagcgac cgggcatcac atcagggtgt
    10021 aatgtattaa cgcttgtaca acggaccgta gttctggcaa gcgcggtaat cctggccttc
    10081 aatatccatg ccgtgcgcag cccaggcaga aggacgatac actttggtct cttcaacgtt
    10141 gtgcatacat accgggatac gcagcatgga ggcaagggtg ataaagtctg cgccaacgtg
    10201 gccgatggtc agaactccgt ggttggcacc ccagttcgcc attaccgaat acacatccgt
    10261 aaacggccct ttaccagtga ggcgcggtgc aaaccaggtg gttggccagg ttgagttggt
    10321 gcgtttgttg aggatgtcat gcacatcctt cggcaattcc acgctccagc cttccgcgat
    10381 ttgcagtacc ggtcccaggc ctttgatgat gttgacacga gtcatggtga acgggacgcc
    10441 gccttcggta aggaagcggg aagagtaacc gccgccacgg aagtattcgt ggatcgccgg
    10501 gcaccattcg gtagcggcga ggcaagcgtc agcctcttgc tgagagattt cccagtgcgg
    10561 cttcatcgtc gggttacctt cgctgtcgcg ttgtttacag gaaccgtcca gcgcagcaga
    10621 acctgagttg atcaaatgga tgatgccgtg ttctgccagt ccgtccagtt tatgccccgt
    10681 tacacgctca attgcttctg gtgaccagta ggtacgcaca tcggcaaata cctgagcggt
    10741 gccggtgagc tggtgaccca ttagcattgc cacgccgtta agactgtcgt tttcggtcgc
    10801 cacgacaaag ggttcgcgca cgccattcca gtcaaatgaa ctgttgagga tcgcttcggc
    10861 ggtgtcacca ttgggatatt gatcggtcca gtgacgttgc ccctggaagc ccgcagcgat
    10921 ggcgttgtag ccaagtgatt cttccacgcg accaatatcg gccagtttgc tgttgccttg
    10981 catcatgtcg cggatacaca tcgccatcag taaactttcg cgcagaactg cgcggctttg
    11041 ctcggcattg cgttgatact gtttgttatt ttcatcttcg ccatagcgga agtttttatc
    11101 agcccaggcc agtgccattt ccagttcggc ttcgtcgtaa atcttctgat cgatacggcg
    11161 acgcagttcg gtcatatcca ccgcctggac tttcattccc agccaggatt caaagaagtt
    11221 gtgatcaaca atagaaccgg cgatacccat cgaaacgccg cccagcgaca gatagctttt
    11281 acctttcatg ctggcgacgg ccaaaccggc gcgggcaaag cgcagcagtt tttcttcaac
    11341 atcggcagga atcgatgtgt catcggcatc ctgaacgtca tgaccgtaaa tggagaatgc
    11401 cgggatgcct ttctggctgt gagctgccag agccgcagcc aggtaaacag cgccggggcg
    11461 ttcagtgccg ttaaagcccc aaatggcttt cgggcgggtt ggatccatgt cgatggtttc
    11521 actgccatag caccagcaag gcgttaccgt aatggtgagg cctacattct ggctgctgaa
    11581 tttttcttcg caagcagcgg cttcagccat acccgcgata caggtatcgg aaatgacaca
    11641 ctcgacggca gctccgcagg catggcgcag tttctcggtc agcagtgcgg ccgtagcttt
    11701 cgccatattc attgtttgtt cttcaagcga ctcacgaaca cccatgcgac gaccgtcaat
    11761 aaccgggcgg ataccaattt tcggtaagct gatttttttc attacagatt cctcacttta
    11821 ttcggaaaat atgttcagtt agttgccgtt tgagaacgga aacgggcaaa gataaagatg
    11881 accgcgaagc agagtgcggg gatcagttca gcagtgggga tgttgcccgc cgcgtcactg
    11941 acaaaaccca tgaccggagt gacaataccg ccgccaataa tggtcataac gatgaaggac
    12001 gaaccgtatt tggtgtcctg gccgagattc ttaatgccca gcgagaagat tgttgggtac
    12061 tgaatcgaca taaaggcgct gcataaagtc agggctatta agcccacatg accgccagcg
    12121 aaggctgaga tcaggcacag tgccatagcg attaatgcgt aggcggccag gactttgtgt
    12181 ggtgcgaagc gactgatgag ccaggtaccg gtgaaacgac caataaagaa gcacaccatg
    12241 gttccggtta aatagttagc ggcaaagcct gcagtcatac ctagaatttc ttctacagcg
    12301 tagcgaatca aatagctcca gcaggccgtt tgtgcgccga catagcagaa ttgcgctaat
    12361 accgcccagc gccagtgacg aatacgcgcc agacgagaaa gcgatgcgga gaacgatcct
    12421 tgtttggcgt cactgtgatt atcactctgc aatgccggga atttcgtcag catgatcagc
    12481 agggcgacca gtaacacgat agccacgatg atcatataag gtgtctgtac cgataatacc
    12541 aggctgtgtt tatacgcact caattgctct ggagacattt tatcgagaac gtcttgcgat
    12601 tgatgtggca cgttagacaa aataagactt tgcccaaaga caaccgcgat aattgcgcca
    12661 aacgagttaa atgtttgcgc aagatttaag cggaagtgac tactgctttc cggccctaat
    12721 accgtaacaa aagggtttgc ggcagtttcc agacaaccta atccggctgc aataataaat
    12781 aggccaacta aaaataaggt gtagttcatt atttctgcag cgggccagaa taatgcagcg
    12841 cccaaggcat ataaaaataa tccggtaata atccctgctt tataactgag ttttttcatc
    12901 aatatcccag cagggattgg gataatgaaa taaccaaagt aaaaggccga ttggatcagg
    12961 ccagcctgga aatttgtcag cgtaaaagcc tgctggaatt gaggtaataa aatgtcgtta
    13021 aggttattgg ctaccgccca aagaaaaaac agtgagcaca gcagcgcgaa tggaataatg
    13081 taacttctgc tttgccctgc atctttatct accgcacggt aactctgcgt ttgtattgat
    13141 gtgtttccca tagcatcctc ttaggttcag aagcttaagc acgtaatcgg ttatgttttc
    13201 aaaagtgcaa aaccagcgag ttcgggcttc atatcggtga atatttgcct gtctgaaatg
    13261 aactcatgga ttcattttca aataaaaaac gctctgtaat atgacggcgg tcacacttat
    13321 tagctggaag gtgactttat gtgactacca tcactttaat aatcgaaatt tcatgcccgt
    13381 ttcagaaaat aaatcataaa aaacacaaac cagatgaccg tattaattgt ttgtgccgaa
    13441 aacgggcatt tctggcgagg ggaaatggaa aaatgaccgt tttgataaat atttttaaga
    13501 gcggtcattt ttaacggggt tataaaaaga gaacttgcag ttatttactg tgattagttg
    13561 agctaggtca caaaatataa gcttgtgaat ttctttcttg ttaatcagga agaagatggt
    13621 gggtaattaa acggctaatt caatagtgtg aaaggaacaa cattattgcc ctgttttgaa
    13681 tcagagagag gtagctaatg gaacgaaata aacttgctcg tcagattatt gacacttgcc
    13741 tggaaatgac ccgcctggga ctgaaccagg ggacagcggg gaacgtcagt gtacgttatc
    13801 aggatgggat gctgattacg cctacaggca ttccatatga aaaactgacg gagtcgcata
    13861 ttgtctttat tgatggcaac ggtaaacatg aggaaggaaa gctcccctca agcgaatggc
    13921 gtttccatat ggcagcctat caaagcagac cggatgccaa cgcggttgtt cacaatcatg
    13981 ccgttcattg cacggcagtt tccattctta accgatcgat ccccgctatt cactacatga
    14041 ttgcggcggc tggcggtaat tctattcctt gcgcgcctta tgcgaccttt ggaacacgcg
    14101 aactttctga acatgttgcg ctggctctca aaaatcgtaa ggcaactttg ttacaacatc
    14161 atgggcttat cgcttgtgag gtgaatctgg aaaaagcgtt atggctggcg catgaagttg
    14221 aagtgctggc gcaactttac ctgacgaccc tggcgattac ggacccggtg ccagtgctga
    14281 gcgatgaaga gattgccgta gtgctggaga aattcaaaac ctatgggtta cggattgaag
    14341 agtaatttcg taaagcaaca aggagaagga tgatggctaa cagaatgatt ctgaacgaaa
    14401 cggcatggtt tggtcggggt gctgttgggg ctttaaccga tgaggtgaaa cgccgtggtt
    14461 atcagaaggc gctgatcgtc accgataaaa cgctggtgca atgcggcgtg gtggcgaaag
    14521 tgaccgataa gatggatgct gcagggctgg catgggcgat ttacgacggc gtagtgccca
    14581 acccaacaat tactgtcgtc aaagaagggc tcggtgtatt ccagaatagc ggcgcggatt
    14641 acctgatcgc tattggtggt ggttctccac aggatacttg taaagcaatt ggcattatca
    14701 gcaacaaccc ggagtttgcc gatgtgcgta gcctggaagg gctttccccg accaataaac
    14761 ccagtgtacc gattctggca atccccacca cagcaggtac tgcggcagaa gtgaccatta
    14821 actacgtgat cactgacgaa gaaaaacggc gcaagtttgt ttgcgttgat ccgcatgata
    14881 tcccgcaggt ggcgtttatt gatgctgaca tgatggatgg tatgcctccg gcgctgaaag
    14941 ccgcgacggg tgttgatgcg ctcactcatg ctattgaggg gtatattacc cgtggcgcgt
    15001 gggcactaac cgatgcactg cacattaaag cgattgaaat cattgctggg gcgctgcgag
    15061 gatcggttgc tggtgataag gatgccggag aagaaatggc gctcgggcag tatgttgcgg
    15121 gtatgggctt ctcgaatgtt gggttagggt tggtgcatgg tatggcgcat ccactgggcg
    15181 cgttttataa cactccacac ggtgttgcga acgccatcct gttaccgcat gtcatgcgct
    15241 ataacgctga ctttaccggt gagaagtacc gcgatatcgc gcgcgttatg ggcgtgaaag
    15301 tggaaggtat gagcctggaa ggggcgcgta atgccgctgt tgaagcggtg tttgccctca
    15361 accgtgatgt cggtattccg ccacatttgc gtgatgttgg tgtacgcaag gaagacattt
    15421 cggcactggc tcaggcggca ctggatgatg tttgtaccgg tggcaacccg cgtgaagcaa
    15481 cgcttgagga tattgtagag ctttaccata ccgcctggta aatgcgctga tgtgataatg
    15541 ccggatacga tttgcggcgt atccggctcg ccgttaccgt accaaccgca attgctgtaa
    15601 attgccgtcg atatgcaaat cggtttgtaa gcgggcaata tcgcggcaca gaaacgccat
    15661 ctctttatgg gtttctaatt ttttgcgcca cttttcggca accgcatcca gattctcata
    15721 tatcccttcc agactctgaa actcgaccag cagctgcgtg gcgctttttg gtccgattcc
    15781 cgcaacaccc ggtacctttg aactgctgat ccccgcaagt ccccagtaat cgggcaactg
    15841 ctgcggttga acgccaaatt ctttatcgat aaatggcgca tccagccaac gtttctggaa
    15901 gtaatcacga atacgtaatg tcggtgaaag taactgacag tagcctttat ctgtcgaaac
    15961 aatcgttgcc tgatgcccgg cctgtgtcac tttgaccgcc agtgtggcgg ctaagtcatc
    16021 ggcttcgttg ccgctggctg accagcacgg gacgccgcgt tgctcaaagg cggcgcgtaa
    16081 tgcaggcatc tcgtcgtgca actcttccgg cattggcggt cgatccgctt tgtaatctgg
    16141 taaacgctga tgacgccagc cgctactgcg gttttcatca tcaaaaacgg cgaccgcgtg
    16201 ggttggctgg ctgtgcataa tgagctgatc gagcgcatgc tggcaggttt cgacacaggg
    16261 cgaaccctga acggcatgaa tgcgacggat aagattcagt gcatcgacaa taagcaaatg
    16321 aacagccacg ataaccccct taaagatcag gcgataaggg taacattcga tgccggaaca
    16381 ggctatggct ggttgagaaa acaggaagag gcttcgcaaa acgaggcctc tgggagagat
    16441 taatcgcagg caacgatctt catcgccagg ccgccgcgag aggtttcgcg gtacttggcg
    16501 ttcatgtctt tacctgtttc gtacatggtt tcgataactt tatcgaggca gacgcgcggt
    16561 tcgctggtac ggcgcagtgc catacgcgcg gcgttcaccg ctttcactgc tgcaatggcg
    16621 ttacgctcaa tgcacggtac ctgaacctgc cccgcgaccg ggtcacaagt cagaccgagg
    16681 ttgtgctcca tcgcgatttc cgctgcgatg caaacctgcg ccgggcttgc gcctaacagt
    16741 tctgccagac cagctgccgc catagagcac gccacgccaa cttcaccctg gcaacccact
    16801 tcggcaccag aaatcgacgc gttcatctta taaagagaac caatggcgct ggctaccagc
    16861 aggtaacgag ccagtgagtt agcgttcact tcgcggataa acttatcgta gtacgccagc
    16921 acagccggga taatcccgca cgcaccgtta gtcggcgcag tcaccacgcg accgccagca
    16981 gcgttctctt cgttcactgc cagtgcaaac atgttgatcc agtcaacaac cgccatcggg
    17041 tcagtggtgg ttttatcctg gctgaccagc atccggcgta gtgccgcagc acggcgtgga
    17101 acgcgcagtt tgccaggcaa cacgccttcg gtggaaatac cgcgctcaat accgccacgc
    17161 atgacttccc agacgttcgc caggtgctgt tccagctctt ctttactgtg cagcgccagc
    17221 tcgtttttca tcatcaggcc agagagtgac agcccggttt cctgacaatg tttttgcaga
    17281 tcggctgctg aactgtacgg ataaggaact tcaaccggtg cgctatcctg ctggccaaaa
    17341 tgctcttcat caacgataaa gccaccgcca atagagtagt aagtctggct gtaaacgact
    17401 ttatcgcccg ccagcgcggt aatgcgcata ccgttttcat gcagagaaag gttgtctgca
    17461 tggaagttca tgcactgatc aaccgggaac tccacttcat gctgaccgtt tgccagcatc
    17521 aggcgaccat gagtattcac atcctgaata aaaccgggga tggaatcgat atccacggta
    17581 tccggcaggt tacccgccag gcccataata atggcgatat ctgtgtggtg gcctttacca
    17641 gtcagagaga gcgagccgta cacgtcaacc accacgcggg tcacgtcttt aagcaggtta
    17701 cgggcaatca gatcgtcggt aaattgttta cccgctttca ttggtccaac ggtatgagaa
    17761 ctggaagggc caatgccgat tttgaaaata tcgaatacgc taatcatagg aaatacatcg
    17821 cgttaaaacg gaggaagcgc cgcccgaaag cggcgcgaaa ggacttagct gaacagagag
    17881 tagaagattg cggagattgc aatcagaccc atcacgacaa cgaatacgtt gctgatgtga
    17941 ccgctgtact tacgcattgc cggtactttc tgaattgcgt acatcggcat caggaacagg
    18001 atcatcgcga tgattgggcc gcccagggtt tcaatcatac ccaggatgct cgggttcagg
    18061 gtggcaacaa tccaggtcgt taccagcatg aacagcgcag tgatacggtt cagcttgttg
    18121 atttcgatag acttaccttt accacgcaga gatttaatca ccataccgtt gaagccttca
    18181 cgtgcgccca ggtagtggcc gaggaaggat ttggtgatag cgataatcgc gataatcgga
    18241 gccatccacg cgataaccgg tgcgttaaag tggttagcca ggtaagacag aatcgagatg
    18301 ttctgctctt tagccgcagc caggtctgcc ggagtcaggc tcagtacgca gctgaagacg
    18361 aagaacatta cggtcagcac catcatgatg tgtgcgaatg ccaggatctt cgagcatttc
    18421 tgttctgcca tatcgccgta ctcttcacgc ttcgcaacgg cgaaagaaga gatgatcgga
    18481 gagtggttga acgagaacac cattaccgga attgccagcc acagggtcat ccacagaccg
    18541 tttccggttg cagatgcagt gtccagagac agcgtttcca gtgctgcgcc gttccactgc
    18601 gggatcaggt acagagccag cagcatcagt acgccaacaa acgggaatac cagaatactc
    18661 atcgctttaa cgatcatctg ctcaccaaag cgaacgatgg tcatcatacc cacgatcagg
    18721 atcagcgaca gaatcgcacg cggcggtggc gtcataccca gctggtgaga catgaagctt
    18781 tcaacggtat tggtgattgc cacgctataa accagcagga tcgggtagat agcgaagaag
    18841 tagagcaggg taatcagttt acctgcgcca ataccaaagt gttcttctac aacctcggtg
    18901 atgtcttcgc ccgggttttt accagacagt acgaagcgag tcaggccgcg gtgagcaaaa
    18961 aacgtcatcg ggaacgcaag gatagccatg atgatcagcg ggatcatacc gccaacaccg
    19021 gcgttgattg gcaggaacag cacgcccgcg ccgattgccg tgccgtaaag gcccagcatc
    19081 cacatggtgt ctgtcttgcg ccaggcacta cgagagtctt tcgacgcaat cgtgctggtt
    19141 tgagtcgttt ccatctattt ctcctggagg aatgcaaata attcagcttt tagccagttc
    19201 agcccgggaa ggtgcctgac cgtaaaaatg aaatctgtct atcggcgatg gcttaatcat
    19261 ttaccgccgt aataatttga gggcggaaag atacacacaa gttatgtatc tatcagtgat
    19321 cttgatccca aatacaataa tctgcttgga agatagatta tcccaacgct ttactctgta
    19381 aatcaaaagt aaaagagaat ttaagggcaa tatttgccca ttaaacgaca tctgacttct
    19441 tccggggcgc gcaatatatg taggttgaac tgaaaaaatc aatactttta gttaatcatg
    19501 gcgataattt ctttttcata gcgataaaat acggtttatg aaaataggga aacgtttgtg
    19561 ttgcaacata aattgtatga catattaact gtgatgat
//
LOCUS       DAJTRC010000074        19259 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.074, whole genome shotgun sequence.
ACCESSION   DAJTRC010000074 DAJTRC010000000
VERSION     DAJTRC010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19259
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.074"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO357_002807"
     CDS             complement(151..1584)
                     /gene="hldE"
                     /locus_tag="OO357_002807"
                     /EC_number="2.7.1.167"
                     /EC_number="2.7.7.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase/D-glycero-beta-D-manno-heptose 1-phosphate
                     adenylyltransferase HldE"
                     /protein_id="HCR5887694.1"
                     /translation="MKVTLPEFERAGVMVVGDVMLDRYWYGPTSRISPEAPVPVVKVN
                     TIEERPGGAANVAMNIASLGANARLVGLTGIDDAARALSKSLADVNVKCDFVSVPTHP
                     TITKLRVLSRNQQLIRLDFEEGFEGVDPQPLHERINQALSSIGALVLSDYAKGALASV
                     QQMIQLARKAGVPVLIDPKGTDFERYRGATLLTPNLSEFEAVVGKCKTEEEIVERGMK
                     LIADYELSALLVTRSEQGMSLLQPGKAPLHMPTQAQEVYDVTGAGDTVIGVLAATLAA
                     GNSLEEACFFANAAAGVVVGKLGTSTVSPIELENAVRGRADTGFGVMTEEELKLAVAA
                     ARKRGEKVVMTNGVFDILHAGHVFYLANARKLGDRLIVAVNSDASTKRLKGDSRPVNP
                     LEQRMIVLGALEAVDWVVSFEEDTPQRLIAGILPDLLVKGGDYKPEEIAGSKEVWANG
                     GEVLVLNFEDGCSTTNIIKKIQQDKKG"
     gene            complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO357_002808"
     CDS             complement(1632..4472)
                     /gene="glnE"
                     /locus_tag="OO357_002808"
                     /EC_number="2.7.7.42"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamate--ammonia
                     ligase]-adenylyl-L-tyrosine
                     phosphorylase/[glutamate--ammonia-ligase]
                     adenylyltransferase"
                     /protein_id="HCR5887695.1"
                     /translation="MKPLSSPLQQYWQTVVERLPELLAEESLSAQAKSVLTFSDFVQD
                     SVIAHPEWLTELESQPPQADEWQHYSVWLQEALSNVSDEAGLMRELRLFRRRIMVRIA
                     WAQTLALVTEESILQQLSYLAETLIVAARDWLYDACCREWGTPCNAQGEAQPLLILGM
                     GKLGGGELNFSSDIDLIFAWPEHGCTQGGRRELDNAQFFTRMGQRLIKVLDQPTQDGF
                     VYRVDMRLRPFGESGPLVLSFAALEDYYQEQGRDWERYAMVKARIMGDSEGVYANELR
                     AMLRPFVFRRYIDFSVIQSLRNMKGMIAREVRRRGLTDNIKLGAGGIREIEFIVQVFQ
                     LIRGGREPLLQSRSLLPTLSAIAALHLLSENDAEQLRVAYLFLRRLENLLQSINDEQT
                     QTLPSDELNRARLAWAMDFADWPQLTGALTAHMTNVRRVFNELIGDDESETQEESLSE
                     QWRELWQDALQEDDTTPVLAHLSEDDRKQVLTMIADFRKELDKRTIGPRGRQVLDHLM
                     PHLLSDVCAREDAAVTLSRITALLVGIVTRTTYLELLSEFPAALKHLISLCAASPMIA
                     SQLARYPLLLDELLDPNTLYQPTATDAYRDELRQYLLRVPEDDEEQQLEALRQFKQAQ
                     LLRIAAADIAGTLPVMKVSDHLTWLAEAMIDAVVQQAWGQMVARYGKPNHLNEREGRG
                     FAVVGYGKLGGWELGYSSDLDLIFLHDCPMDAMTDGEREIDGRQFYLRLAQRIMHLFS
                     TRTSSGILYEVDARLRPSGAAGMLVTSAEAFADYQKNEAWTWEHQALVRARVVYGDPQ
                     LTAHFDAVRREIMTLPREGKTLQTEVREMREKMRAHLGNKHRNRFDIKADEGGITDIE
                     FITQYLVLRYAHEKPKLTRWSDNVRILELLAQNDIMEEQEAMALTRAYTTLRDELHHL
                     ALQELPGHVSEDCFTAERDLVRASWQKWLVEE"
     gene            complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO357_002809"
     CDS             complement(4495..5796)
                     /gene="ygiF"
                     /locus_tag="OO357_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic triphosphatase"
                     /protein_id="HCR5887696.1"
                     /translation="MAQEIELKFIVNHSAVEALRDHLNTLGGEHHDPVQLLNIYYETP
                     DNWLRRHDMGLRIRGENGRYEMTMKVAGRVTGGLHQRPEYNVALSEPTLDLAQLPTEI
                     WPNGELPADLASRVQSLFSTDFYREKWLVEIDGSRIEIALDQGEVKAGEFAEPICELE
                     LELLSGDTRAVLKLANQLVSQTGLRQGSLSKAARGYHLAQGNPAREIKPTTILHVAAK
                     ADVEQGLEAALELVLAQWQYHEELWVRGNDAAKEQVLAAISLVRHTLMLFGGIVPRKA
                     STHLRDLLTQCEATIASAVSAVTAVYSTETAMAKLALTEWLVSKAWQPFLDAKAQGKI
                     SDSFKRFADIHLSRHAAELKSVFCQPLGDRYRDQLPRLMRDIDSILLLAGYYDPVVAQ
                     AWLENWQGLRHAIATGQRIEIEHFRNEANNQEPFWLHSGKR"
     gene            6038..6658
                     /locus_tag="OO357_002810"
     CDS             6038..6658
                     /locus_tag="OO357_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HCR5887697.1"
                     /translation="MPKLRLIGLTLLALSATAVSHAEETRYVSDELNTWVRSGPGDHY
                     RLVGTVNAGEEVTLLQTDANTNYAQVKDSSGRTAWIPLKQLSTEPSLRSRVPDLENQV
                     KTLTDKLTNIDNTWNQRTAEMQQKVAQSDSVINGLKEENQKLKNELIVAQKKVDAASV
                     QLDDKQRTIIMQWFMYGGGVLGLGLLLGLVLPHLIPSRKRKDRWMN"
     gene            6722..7960
                     /gene="cca"
                     /locus_tag="OO357_002811"
     CDS             6722..7960
                     /gene="cca"
                     /locus_tag="OO357_002811"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused tRNA nucleotidyltransferase/2',3'-cyclic
                     phosphodiesterase/2' nucleotidase/phosphatase Cca"
                     /protein_id="HCR5887698.1"
                     /translation="MKIYLVGGAVRDALLGLSVKDRDWVVVGSTPQEMLDAGYQQVGR
                     DFPVFLHPQTHEEYALARTERKSGSGYTGFTCYTAPDVTLEDDLKRRDLTINALAQDD
                     NGEIIDPYNGLGDLQNRLLRHVSPAFGEDPLRVLRVARFAARYAHLGFRIADETLTLM
                     REMTHAGELEHLTPERVWKETESALTTRNPQVFFQVLRDCGALRVLFPEIDALFGVPA
                     PARWHPEIDTGIHTLMTLSMAAMLSPQVDVRFATLCHDLGKGLTPPELWPRHHGHGPA
                     GVKLVEQLCQRLRVPNEIRDLARLVAEFHDLIRTFPMLNPKTIVKLFDSIDAWRKPQR
                     IEQLALTSEADVRGRTGFESADYPQGRWLREGWEVAQSVPTKAVVEAGFKGVEIREEL
                     TRRRIAAVASWKEQRCPKPD"
     gene            complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO357_002812"
     CDS             complement(8123..8944)
                     /gene="bacA"
                     /locus_tag="OO357_002812"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HCR5887699.1"
                     /translation="MSDMHSLLIAAILGVVEGLTEFLPVSSTGHMIIVGHLLGFEGDT
                     AKTFEVVIQLGSILAVVVMFWRRLFGLIGIHFGRPLQHEGESKGRLTLIHILLGMIPA
                     VVLGLLFHDTIKSLFNPINVMYALVVGGLLLIAAECLKPKEPRAPGLDDMTYRQAFMI
                     GCFQCLALWPGFSRSGATISGGMLMGVSRYAASEFSFLLAVPMMMGATALDLYKSWGF
                     LTTGDIPMFAVGFITAFVVALIAIKTFLQLIKRISFIPFAIYRFIVAAAVYVVFF"
     gene            complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO357_002813"
     CDS             complement(9035..9403)
                     /gene="folB"
                     /locus_tag="OO357_002813"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional dihydroneopterin
                     aldolase/7,8-dihydroneopterin epimerase"
                     /protein_id="HCR5887700.1"
                     /translation="MDIVFIEQLSVITTIGVYDWEQTIEQKLVFDIEMAWDNRKAAKS
                     DDVADCLSYADIAETVVSHVEGARFALVERVAEEVAELLLARFNSPWVRIKLSKPGAV
                     ARAANVGVIIERGNNLKENN"
     gene            9508..10125
                     /gene="plsY"
                     /locus_tag="OO357_002814"
     CDS             9508..10125
                     /gene="plsY"
                     /locus_tag="OO357_002814"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HCR5887701.1"
                     /translation="MSAIAPGMILIAYLCGSISSAILVCRLCGLPDPRTSGSGNPGAT
                     NVLRIGGKGAAVAVLIFDVLKGMLPVWGAYELGVSPFWLGLIAIAACLGHIWPVFFGF
                     KGGKGVATAFGAIAPISWDLTGVMAGTWLLTVLLSGYSSLGAIVSALIAPFYVWWFKP
                     QFTFPVSMLSCLILLRHHDNIQRLWRRQETKIWTKFKRKREKDPE"
     gene            complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO357_002815"
                     /gene_synonym="dan"
                     /pseudo
     CDS             complement(10138..11069)
                     /gene="ttdR"
                     /locus_tag="OO357_002815"
                     /gene_synonym="dan"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417532.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator TtdR"
     gene            11276..12119
                     /gene="ttdA"
                     /locus_tag="OO357_002816"
                     /pseudo
     CDS             11276..12119
                     /gene="ttdA"
                     /locus_tag="OO357_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417533.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit alpha"
     gene            12116..12721
                     /gene="ttdB"
                     /locus_tag="OO357_002817"
     CDS             12116..12721
                     /gene="ttdB"
                     /locus_tag="OO357_002817"
                     /EC_number="4.2.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L(+)-tartrate dehydratase subunit beta"
                     /protein_id="HCR5887702.1"
                     /translation="MKKILTTPIKAEDLQDIRVGDVIYLTGTLVTCRDVCHRRLIELK
                     RPIPYDLNGKAIFHAGPIVRKNGDKWEMVSVGPTTSMRMESFEREFIEQTGVKLVVGK
                     GGMGPLTEEGCQKFKALHVIFPAGCAVLAATQVEEIEEVHWTELGMPESLWVCRVKEF
                     GPLIVSIDTHGNNLIAENKKLFAERRDPIVEEICEHVHYIK"
     gene            12769..14232
                     /gene="ttdT"
                     /locus_tag="OO357_002818"
     CDS             12769..14232
                     /gene="ttdT"
                     /locus_tag="OO357_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-tartrate/succinate antiporter"
                     /protein_id="HCR5887703.1"
                     /translation="MKPSTEWWRYLAPLAVIAIIALLPVPAGLENHTWLYFAVFTGVI
                     VGLILEPVPGAVVAMVGISIIAILSPWLLFSPEQLAQPGFKFTAKSLSWAVSGFSNSV
                     IWLIFAAFMFGTGYEKTGLGRRIALILVKKMGHRTLFLGYAVMFSELILAPVTPSNSA
                     RGAGIIYPIIRNLPPLYQSQPNDSSSRSIGSYIMWMGIVADCVTSAIFLTAMAPNLLL
                     IGLMKSASHATLSWGDWFLGMLPLSILLVLLVPWLAYVLYPPVLKSGDQVPRWAETEL
                     QAMGPLCSREKRMLGLMVGALVLWIFGGDYIDAAMVGYSVVALMLLLRIISWDDIVSN
                     KAAWNVFFWLASLITLATGLNNTGFISWFGKLLAGSLSGYSPTMVMVTLIVVFYLLRY
                     FFASATAYTCALAPMMIAAALAMPEIPLPVFCLMVGAAIGLGSILTPYATGPSPIYYG
                     SGYLPTADYWRLGAIFGLIFLVLLVITGLLWMPVVLL"
     gene            complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO357_002819"
     CDS             complement(14275..15288)
                     /gene="tsaD"
                     /locus_tag="OO357_002819"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001264383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HCR5887704.1"
                     /translation="MRVLGIETSCDETGIAIYDDEKGLLANQLYSQVKLHADYGGVVP
                     ELASRDHVRKTVPLIQAALKESGLTAKDIDAVAYTAGPGLVGALLVGATVGRSLAFAW
                     NVPAIPVHHMEGHLLAPMLEDNPPEFPFVALLVSGGHTQLISVTGIGQYELLGESIDD
                     AAGEAFDKTAKLLGLDYPGGPLLSKMAAQGTAGRFVFPRPMTDRPGLDFSFSGLKTFA
                     ANTIRDNGTDDQTRADIARAFEDAVVDTLMIKCKRALDQTGFKRLVMAGGVSANRTLR
                     AKLAEMMKKRRGEVFYARPEFCTDNGAMIAYAGMVRFKAGATADLGVSVRPRWPLAEL
                     PAA"
     gene            15526..15741
                     /gene="rpsU"
                     /locus_tag="OO357_002820"
     CDS             15526..15741
                     /gene="rpsU"
                     /locus_tag="OO357_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HCR5887705.1"
                     /translation="MPVIKVRENEPFDVALRRFKRSCEKAGVLAEVRRREFYEKPTTE
                     RKRAKASAVKRHAKKLARENARRTRLY"
     gene            15852..17597
                     /gene="dnaG"
                     /locus_tag="OO357_002821"
     CDS             15852..17597
                     /gene="dnaG"
                     /locus_tag="OO357_002821"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HCR5887706.1"
                     /translation="MAGRIPRVFINDLLARTDIVDLIDARVKLKKQGKNFHACCPFHN
                     EKTPSFTVNGEKQFYHCFGCGAHGNAIDFLMNYDKLEFVETVEELAAMHNLEVPFEAG
                     SGPSQIERHQRQTLYQLMDGLNTFYQQSLQQPVATSARQYLEKRGLSHEVIARFAIGF
                     APPGWDNVLKRFGGNPENRQSLIDAGMLVTNDQGRSYDRFRERVMFPIRDKRGRVIGF
                     GGRVLGNDTPKYLNSPETDIFHKGRQLYGLYEAQQDNAEPNRLLVVEGYMDVVALAQY
                     GINYAVASLGTSTTADHIQLLFRATNNVICCYDGDRAGRDAAWRALETALPYMTDGRQ
                     LRFMFLPDGEDPDTLVRKEGKEAFEARMEQAMPLSAFLFNSLMPQVDLSTPDGRARLS
                     TLALPLISQVPGETLRIYLRQELGNKLGILDDSQLERLMPKAAESGVSRPVPQLKRTT
                     MRILIGLLVQNPELATLVPPLENLDENKLPGLGLFRELVNTCLSQPGLTTGQLLEHYR
                     GTNNAATLEKLSMWDDIADKNIAEQTFTDSLNHMFDSLLELRQEELIARERTHGLSNE
                     ERLELWTLNQELAKK"
     gene            17792..>19259
                     /gene="rpoD"
                     /locus_tag="OO357_002822"
     CDS             17792..>19259
                     /gene="rpoD"
                     /locus_tag="OO357_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HCR5887707.1"
                     /translation="MEQNPQSQLKLLVTRGKEQGYLTYAEVNDHLPEDIVDSDQIEDI
                     IQMINDMGIQVMEEAPDADDLMLAENTADEDAAEAAAQVLSSVESEIGRTTDPVRMYM
                     REMGTVELLTREGEIDIAKRIEDGINQVQCSVAEYPEAITYLLEQYDRVEAEEARLSD
                     LITGFVDPNAEEDLAPTATHVGSELSQEDLDDDEDEDEEDGDDDSADDDNSIDPELAR
                     EKFAELRAQYVVTRDTIKAKGRSHAAAQEEILKLSEVFKQFRLVPKQFDYLVNSMRVM
                     MDRVRTQERLIMKLCVEQCKMPKKNFITLFTGNETSDTWFNAAIAMNKPWSEKLHDVS
                     EEVHRALQKLQQIEEETGLTIEQVKDINRRMSIGEAKARRAKKEMVEANLRLVISIAK
                     KYTNRGLQFLDLIQEGNIGLMKAVDKFEYRRGYKFSTYATWWIRQAITRSIADQARTI
                     RIPVHMIETINKLNRISRQMLQEMGREPTPEELAERMLM"
BASE COUNT         4577 a         5381 c         4907 g         4394 t
ORIGIN      
        1 gttgttactg ttaagtttcc cgacttacta acaactgatc agagggggga gaaatcctcc
       61 cttacccttg ttcctttact ttaggttgaa aaaacaacag cgtcaatagg cctgcctgct
      121 acgaagcgag atctgtgaac cgctttccgg ttagcctttt ttatcctgtt ggatcttctt
      181 gatgatgtta gtggtcgagc aaccgtcttc gaagttgagc accagcactt cgccgccgtt
      241 ggcccagact tctttactcc cggcgatctc ttctggttta tagtcgccgc ccttcaccag
      301 taaatctggc aggatcccgg cgatcaagcg ctgcggcgtg tcttcttcaa acgacaccac
      361 ccagtcgacc gcttccagtg cgcccagcac aatcatacgc tgttcgagcg ggttaacggg
      421 gcgagaatcc cctttcagcc gtttggtgga ggcatcgctg ttcacagcga caatcaagcg
      481 gtcacccagt ttgcgggcat ttgccagata gaagacgtgt ccggcgtgca ggatgtcaaa
      541 gacgccattg gtcatcacca ctttttcacc gcgtttacgc gctgccgcta cggccagctt
      601 cagttcctct tcggtcatca cgccaaagcc ggtatctgcg cgcccacgca ctgcattttc
      661 cagctcgatc ggcgaaaccg tggaggttcc cagtttgccg accaccacgc cagccgccgc
      721 attggcaaag aagcaggctt cttccagcga attacccgct gccagcgttg ccgccaggac
      781 gccaatcacc gtgtcgcccg caccggtaac gtcatacact tcctgcgcct gggttggcat
      841 atgcagcggc gctttacccg gttgcagcag cgacataccc tgttcggaac gggtcactaa
      901 cagagccgag agttcgtaat cggcaatcag tttcatgccg cgctcaacaa tctcttcttc
      961 ggtcttacat ttaccgacaa cagcttcaaa ttccgagaga ttcggtgtca gcagcgtagc
     1021 gccgcggtag cgctcaaaat cggtaccttt tggatcaatc agcaccggaa cgcccgcttt
     1081 acgcgccagt tggatcatct gctgtacgct tgccagcgca cctttggcgt agtcagaaag
     1141 caccagcgcg ccaatcgaac tcagcgcctg attaatccgc tcgtgcagcg gctgcggatc
     1201 aacaccttcg aaaccttctt caaaatccag acggatcagc tgttggttgc gggaaagtac
     1261 ccgtaatttg gtgatggtcg gatgcgtcgg tacagaaacg aagtcgcatt tgacgttgac
     1321 gtcggccaga gatttactca gcgcgcgcgc tgcatcgtca atgcccgtca acccgaccag
     1381 gcgtgcatta gcaccgagag aagcgatatt catcgccacg ttagccgcgc cgcccggacg
     1441 ttcttcgatg gtattcactt taaccacggg caccggcgct tccggcgaga tacgactggt
     1501 ggggccgtac cagtaacgat ccagcatcac atcaccaacc accatcactc ctgcacgttc
     1561 aaactctggc agcgttactt tcattcctgt ctcctgagag attcaaaatt tgcgcgcgat
     1621 aataccatac ttcattcttc caccagccac ttctgccagc ttgcccgcac cagatcacgc
     1681 tctgcggtga agcaatcctc cgacacatgg cccggcaatt cctgtaatgc cagatgatga
     1741 agttcatcgc gcagcgtcgt gtaagcacgg gtcagcgcca tcgcttcctg ctcttccata
     1801 atgtcgtttt gcgccagcag ctccagaata cgcacgttgt ctgaccagcg cgttaacttc
     1861 ggtttttcat gagcgtagcg caacaccaga tattgggtaa taaattcgat atcggtaatt
     1921 ccgccttcat cggctttgat atcaaagcga ttacgatgtt tattgccgag atgagcgcgc
     1981 attttctcgc gcatttcccg cacttccgtt tgcagagttt taccttctcg cggcagtgtc
     2041 ataatctcac gacgcaccgc gtcaaagtgc gcagtgagct gcggatcgcc gtacactaca
     2101 cgcgcacgca ccagcgcctg atgttcccac gtccaggcct cgtttttctg ataatcggca
     2161 aatgcttctg cggatgtcac cagcattccc gccgccccgg acggacgcag tcgagcatcc
     2221 acttcataca aaatgccgga agaggtacgc gtactgaaca gatgcataat gcgttgcgcc
     2281 agacgcagat aaaactgccg cccgtcgatt tcccgctcgc cgtcagtcat cgcatccatt
     2341 gggcaatcat ggaggaagat aaggtcaaga tcggaactgt agcctaactc ccagccgccc
     2401 agcttgccgt agccgaccac cgcaaaaccg cgcccttcac gttcgttgag gtgatttggc
     2461 ttaccgtagc gggccaccat ttgaccccac gcctgctgaa cgacggcatc tatcatagct
     2521 tctgccagcc aggttaagtg atcgctcact ttcattaccg gtagcgtacc ggcgatatcc
     2581 gctgccgcaa tgcgtaacag ctgcgcctgt ttgaactgac gcagcgcctc aagctgttgt
     2641 tcttcgtcat cttccggcac gcgcagcaaa tactggcgca gttcatcacg gtaggcatcg
     2701 gtcgccgtcg gctgataaag ggtatttgga tcgagcaatt catccagcaa taatggataa
     2761 cgcgctagct ggctggcaat catcggcgat gcggcacaca gagaaatcaa atgtttaagc
     2821 gccgcaggga attcactgag caattctaaa taggttgtgc gggtaacaat ccccaccagc
     2881 aaggcggtaa tgcgcgacag cgtgacagca gcgtcttcac gcgcacagac atcacttagc
     2941 agatgtggca tcagatggtc gagcacctga cgtcctcgcg gcccgatagt acgcttatcc
     3001 agctctttgc ggaaatcagc aatcatcgtt aggacctgtt tgcgatcatc ctcgctaaga
     3061 tgcgccagca ctggcgtagt gtcatcttcc tgcaacgcat cctgccacag ctcacgccac
     3121 tgttccgaca gcgactcttc ctgagtttca ctttcgtcgt cgccaatcaa ttcattaaac
     3181 acccggcgca cattggtcat atgtgcggtc agcgccccgg tcagttgcgg ccagtcagca
     3241 aagtccatcg cccacgccag ccgcgcacgg ttaagctcat cagaaggaag cgtttgggtt
     3301 tgttcgtcgt taatgctttg cagcaggttt tccagacgcc gcaggaacag atatgccact
     3361 cgcaattgtt cagcatcgtt ttcagaaagc agatgcagcg cggcaatggc gctgagcgtt
     3421 ggcagtaaag agcgcgactg cagcaacggt tcgcgcccac cgcgaatgag ctggaacacc
     3481 tgaacgataa attcaatttc gcgaatgccg cctgcgccga gtttgatatt gtcggtcaaa
     3541 ccacgtcgac gcacttcacg ggcaatcatc cctttcatgt tgcgcagcga ctgaatcacg
     3601 ctgaaatcga tgtaacgacg gaaaacaaac gggcgcagca tcgcacgcaa ctcgttagca
     3661 tagacgcctt cgctatcgcc cataatccgc gctttgacca tcgcgtaacg ctcccagtcg
     3721 cgtccctgct cctggtaata atcctccaac gcggcaaagc tcagcaccag cgggccactt
     3781 tcgccaaacg gacgcagtcg catatccacg cgatagacga agccatcctg cgttggttga
     3841 tccagcactt taatcagccg ctgccccatg cgggtaaaaa actgcgcgtt atccagttcc
     3901 cggcgtccgc cctgcgtaca accatgttcc ggccaggcaa aaatcagatc gatatcagag
     3961 gagaaattca gttccccgcc gcccagctta cccatgccta agatcagcag cggttgcgct
     4021 tcgccctgcg cattgcacgg cgttccccac tcgcggcagc aggcgtcata cagccagtca
     4081 cgcgccgcaa caatcagcgt ctccgccaga taactgagct gctgcaatat gctctcttca
     4141 gtaaccagtg ccagcgtttg cgcccatgcg atgcgcacca taatgcgccg ccggaatagc
     4201 cgcagctcgc gcattaaccc ggcttcgtcg ctcacattac tgagcgcctc ctgcaaccac
     4261 acgctgtaat gttgccattc gtcagcctgt ggcggttggc tttccagttc cgtcagccac
     4321 tctggatgcg caatcacgct gtcctgcaca aaatcactga aagtaagtac tgacttcgcc
     4381 tgtgcactaa gtgattcctc ggctaaaagc tctggcagcc gctcaacaac ggtctgccag
     4441 tactgctgta acggtgaaga gagcggcttc ataagcgatt ttatccttgc ctgattaacg
     4501 ttttccgctg tgcaaccaga acggttcctg attgtttgcc tcattacgga aatgttcaat
     4561 ttcaatgcgt tgcccggtcg caatagcgtg acgcagcccc tgccagttct ccagccaggc
     4621 ttgcgcgacg acaggatcat agtaacccgc cagcaacagt attgagtcaa tatcacgcat
     4681 caggcgtggc aactggtcac ggtagcgatc gcctaacggc tggcagaaaa cgcttttcag
     4741 ttcagcggca tggcgggaaa gatggatatc ggcaaagcgt ttgaaggagt cgctaatttt
     4801 gccctgcgct ttggcatcta aaaacggctg ccatgctttg cttaccagcc attcggtcaa
     4861 cgccagcttc gccattgccg tttcggtaga gtagaccgcc gtcacggcag aaaccgcaga
     4921 agcgatggtc gcttcgcatt gagtcagcaa atcacgtaag tgagtactcg ctttacgcgg
     4981 cacaatgcca ccgaacagca tcagtgtatg acggactagg ctaatggctg ccagcacctg
     5041 ctctttcgcc gcatcgttgc cgcgtaccca cagttcttca tgatactgcc attgcgctaa
     5101 caccagctcg agcgccgctt ccagcccctg ttccacatcg gcttttgccg caacatgcaa
     5161 aatggtggtc ggtttgattt cacgcgccgg attgccctgc gccagatgat agccacgcgc
     5221 cgctttgctc aggctgccct ggcgtaatcc ggtttgcgat accagttggt tcgccagttt
     5281 cagcaccgcg cgcgtgtcgc cgctaagcag ctccagttcc agctcgcaga taggctcagc
     5341 aaattcaccc gctttcactt ccccctggtc gagagcgatt tcaattcggc tgccatcgat
     5401 ctctactagc catttttcgc gataaaaatc ggtgctaaac agcgactgca cgcgggaagc
     5461 gagatcggca ggcaattcgc cgttcggcca gatttccgtc ggtaactgcg ccaggtcgag
     5521 cgtcggttcg ctcaacgcca cgttatattc cgggcgctgg tgtaagccgc ctgtcactct
     5581 tcctgcaact ttcatggtca tctcatagcg accgttttcg ccacgaatac gtaagcccat
     5641 atcgtgccta cgcagccagt tatccggcgt ttcgtagtaa atattcagca actgcacggg
     5701 gtcatggtgc tcgccgccca gcgtattgag atggtcacgc aacgcctcaa cggcactgtg
     5761 attaacaata aactttaatt cgatttcctg agccatggcc tttacttatc ggttatgtca
     5821 cataagtgac gatgaacggc gaacttaatg cgatcttttt tgtcagtaga tagtattttg
     5881 cgccaaattg ccatgcaacg ggcaatttga cgggcgtaaa agtttgaagc agtggcaaga
     5941 cctgacacag aggattccga ttgatgtcga atgctttgcg tagcgacact gatgccacta
     6001 ctatcgttcc actttccatg acaataacga cagcctgatg ccaaaattac gcctgatcgg
     6061 attaacttta ctcgcactta gcgcgactgc cgtctcacac gctgaagaaa cgcgctatgt
     6121 ttctgacgaa ctgaatacct gggtccgtag cggtccggga gatcattatc gcctcgtggg
     6181 cacggttaac gccggcgagg aagtgacctt attacaaact gacgccaaca ccaattatgc
     6241 ccaggtgaaa gacagctctg gccgtaccgc ctggatcccg ttgaaacaac ttagcactga
     6301 gccaagcctg cgctcccgtg tgccagatct ggaaaatcag gtcaaaaccc tgaccgataa
     6361 actcaccaat atcgataaca cctggaatca gcgcaccgca gaaatgcagc aaaaagtggc
     6421 gcagagcgac agcgtgatca acgggttaaa agaagaaaat cagaaactga aaaacgagct
     6481 gattgtcgcg cagaaaaagg tcgatgccgc cagcgtacag ctggatgaca aacagcgcac
     6541 catcatcatg caatggttta tgtatggcgg tggcgtgctg gggcttggct tgctgctcgg
     6601 tctggtactg ccgcacctga tcccaagccg caaacgcaaa gatcgctgga tgaactaaat
     6661 cgccttcttc gttgcactgt cttattatta ggaattgtgt atttgacgaa gggatgatgg
     6721 cgtgaagatt tatctggtcg gtggtgctgt tcgggatgca ttgttaggac tatcggtcaa
     6781 agacagagat tgggtggtgg tcggcagtac gccacaggag atgctcgacg cgggctacca
     6841 gcaggtaggc cgcgattttc ctgtgtttct gcatccgcaa acgcatgaag agtatgcgct
     6901 ggcacgtacc gaacggaaat ccggttccgg ttacaccggt tttacttgct ataccgcacc
     6961 ggatgtcacg ctggaagatg atcttaagcg tcgcgatctg accattaatg cgctggccca
     7021 ggacgataac ggcgagatta tcgacccgta caacggtctg ggcgatctgc aaaatcgtct
     7081 gttgcgccat gtttcccccg cttttggcga agatccgtta cgcgtactgc gcgtggcgcg
     7141 ttttgctgcg cgttatgccc acctcggttt tcgtattgcc gatgaaactc tgacgctgat
     7201 gcgcgagatg acccatgcgg gtgaactgga acacctgacg cctgaacggg tatggaaaga
     7261 gacggaaagc gccctcacca cccgcaatcc ccaggtgttc ttccaggtac tgcgcgattg
     7321 cggcgcactt cgcgttttat tcccggaaat tgacgcactg tttggcgttc cggcacctgc
     7381 caggtggcat ccggaaatcg acacgggtat tcatacctta atgacgctct caatggcggc
     7441 gatgctgagt ccgcaggttg atgtccgttt cgcaacttta tgccacgatc tcggtaaagg
     7501 actgacgccg ccagaacttt ggccgcgtca tcatgggcat ggcccggcgg gtgttaagtt
     7561 agtggaacaa ctatgccagc gtctgcgcgt gccaaatgaa attcgcgatt tagccagact
     7621 ggtggctgag tttcacgatc tcatccgcac cttcccaatg ctgaacccaa aaaccatcgt
     7681 caaattattt gattccatcg atgcctggcg taaaccgcag cgtatcgagc aactggcgct
     7741 gaccagcgag gctgacgtgc gcggcagaac cggttttgaa tcagcggact acccgcaagg
     7801 ccgttggttg cgcgaaggct gggaagtggc gcagtcagtg ccgacaaaag cggtcgttga
     7861 agcgggattt aaaggcgtgg agattcgcga ggagctgacc cgacggcgga ttgcggcggt
     7921 agccagctgg aaggaacaac gttgcccaaa gcctgactga gtataaaact aaacgttagc
     7981 acgaaccgtc ccctcgcccc ttcggggaga gggttagggt gaggggaaca gaccagcacc
     8041 ggtgcgaaca atcaccccca ccccggccct ctccctacag agcgagggag aaaacccggc
     8101 ctgctcccga accttttctc aatcaaaaga acacgacata caccgcagcc gccacaataa
     8161 agcgataaat ggcgaacggg ataaacgaaa tgcgcttaat caattgcagg aaggttttaa
     8221 tcgctatcag cgccaccaca aaagcggtga taaacccaac ggcaaacatc gggatatcgc
     8281 cggttgtcag gaagccccag cttttgtaga gatcgagcgc cgttgcgccc atcatcatcg
     8341 gtaccgccag caggaacgag aactcggaag cagcgtaacg gctcacgccc atcagcatcc
     8401 cacctgaaat ggtcgccccg gaacgggaga aacccggcca cagcgccaga cactggaaac
     8461 agccaatcat aaacgcctga cgataggtca tatcatcaag acccggcgca cgcggctctt
     8521 tcggcttcag gcattcggcg gcaatcagca acaaaccgcc aacgaccagc gcatacatca
     8581 catttatcgg gttaaacaat gacttaatcg tgtcgtggaa taatagcccc agtaccaccg
     8641 ccggaatcat ccccagcaaa atgtggatca gcgttaaacg acctttgctt tcaccttcgt
     8701 gctgcaacgg gcggccaaag tggatgccaa tcaggccaaa cagacgccgc cagaacatca
     8761 ctactaccgc cagaattgat cctaactgga tcacaacttc aaaggttttc gccgtgtcgc
     8821 cctcaaaccc caacaagtga ccgacaataa tcatatggcc cgtgctggat accggcagaa
     8881 attctgtcaa tccttcgacc acacccaata ttgccgctat cagcagcgag tgcatatcgc
     8941 tcatcaataa acccctaaat tattaaaatg taccgcttgt ccgaactact gcagtatgac
     9001 caggttataa ccgtttggtt taacagctgt aaaattaatt attttctttc agattattgc
     9061 cacgctcaat gattacgcca acattcgccg cccgcgccac tgcgcctggc ttgctgagtt
     9121 tgatacgcac ccacggcgag ttgaagcgtg ctaacagcag ctccgccacc tcttcagcca
     9181 cgcgttccac cagcgcaaaa cgcgccccct cgacgtggct gaccaccgtt tctgcaatgt
     9241 cagcgtaact gaggcaatcc gccacatcat cacttttcgc cgctttacgg ttatcccatg
     9301 ccatttcgat atcgaacact aacttctgtt cgatggtctg ttcccagtcg taaacaccaa
     9361 tagtggtgat taccgaaagt tgctctataa atacaatatc catcacgtcc tgcctgcttt
     9421 ttggctaacc ggataccact tccggcgaaa tgtgcgtatt atccacagat tcatcgttga
     9481 acacgaattt tcaaaacgga acagcttatg agtgcaatcg cgcctggaat gatcctcatc
     9541 gcgtacctct gcggctccat ttccagtgcc attctggttt gccgcttgtg tgggctgccc
     9601 gatccgcgaa ccagcggctc cggcaatcca ggcgcaacca atgtgttacg tatcggtggc
     9661 aagggagcag ccgtagcagt actgattttc gacgttctga aaggaatgtt gcctgtctgg
     9721 ggcgcgtatg aattaggtgt cagccccttc tggctaggct taattgccat cgccgcctgt
     9781 cttggacaca tctggcccgt tttcttcggt tttaaaggag gaaaaggcgt tgctaccgct
     9841 tttggagcaa tcgcaccaat tagctgggat ctcaccggag taatggcggg aacctggtta
     9901 ctgaccgtgc tattgagcgg atactcgtcg ctgggagcga ttgtcagtgc actgattgct
     9961 ccgttttatg tctggtggtt taagccacaa ttcaccttcc cggtttcgat gctctcttgc
    10021 ctgatcctgc tgcgtcatca tgacaacatc cagcgtctgt ggcgtcgtca ggagacaaaa
    10081 atctggacga aattcaaaag aaagcgcgaa aaggatcccg agtgatttct ggtggatcta
    10141 catgacctga tagccttcat cgggcttgcc cagccgttgc tggcaccatg ccgccagaaa
    10201 ttccgcgcag acacgaattt catgctgcga tagagcagct cccggtaaac agcccagata
    10261 ttggcgcttt gtgcatactc cggcaatact tgcaccagtt tgccactctc cagaaacggc
    10321 aacacatccc actcggaacg cagcataatc cctttgccct ccagcgccca ttgcaacact
    10381 atctcgccgc tattggagga aagatgcccg ctaaccttca ccgatttttt ctcctgaccg
    10441 ttccccaact cccatattcc gtgggtcata tcgcgctctt tggtcaccag acagtcatga
    10501 cgacttaatt cctgtaatga ttgtggctgc ggatattttt gcagatactc aggtgctgcg
    10561 cataatattc ttttattttt cgttaacaga tgcgcaatat aataatcagg aatttcgtca
    10621 ttaatacgaa tatccagatc aatattatcc tgcactaaat caatttgccg atcgaacaat
    10681 tcaaaatgca cctgtaactc aggataattg cgcataagtt cggtaatggc tggcgcaata
    10741 tggctgcgcc caaaaccaaa actgcaacca atacgaatca tcccctccgg gcgcgttttg
    10801 atttgcgtga cgtcatcgac cagtcgctga tattgggtga ggatttccag cgcatgttcg
    10861 taacaacgtt ggccactttc agtcagcgcc acgccgcgag cggagcggtt cagcagcgtt
    10921 gttgccagcg tattttcaag gatttggatc cgcttggtga caaatgcagg cgtttgccca
    10981 agcgtcgccg ctgcagcact aaaactcccg ctgtggacga tttctaccag tacctgcaag
    11041 tctttggcta aaggccagct attcagcatc gttaattatc cgcagttgtg ataagcgcag
    11101 tgtatttcgc aaaacattgc cattccctgc tctggcgcaa tattcattct tattttcgcg
    11161 ttgattcacg aactgttaat tacatattaa cggtggcaaa aaatataaaa ccacattttt
    11221 agtgttagtt tgtggcggtg aatttttcca gacaaataca aaaactggag ttgccatgat
    11281 gagcgaaagt aataagcaac aggcagtgaa taagttgaca gagattgtcg ctaactttac
    11341 cgccatgatt tctacccgaa tgcctgatga cgtggtggat aaactaaaac agctaaagga
    11401 tgccgaaacg tcgtcgatgg ggaaaattat ctaccatacg atgttcgaca acatgcaaaa
    11461 agcgattgac ctgaatcgtc ctgcctgtca ggacaccggg gagattatgt tcttcgttaa
    11521 agtcggttcc cgcttcccac tgcttggcga gctgcaaagc atactcaaac aagccgtgga
    11581 agaggcaacc gtcaaagcgc cactacgtca caatgcggta gaaatttttg acgaagtaaa
    11641 caccggcaaa aataccggta gcggcgtacc gtgggtcacc tgggacatca tccccgacaa
    11701 tgacgatgcg gaaatcgaag tttacatggc aggcggcggc tgcacgctac ctggccgctc
    11761 gaaagtgtta atgccgtcag aaggctacga aggcgtggtg aaattcgtct tcgaaaatat
    11821 ctccaccctc gccgtaaacg cctgtccacc ggtactggtg ggcgtgggca tcgccacctc
    11881 ggtggaaacc gccgccgtac tctcgcgtaa agccattttg cgcccgattg gcagccgcca
    11941 tcccaatcca aaagcggcag aactggagct acgcctggaa gaaggactca accgtctggg
    12001 gattggtcca caagggctga ccggcaacag ttcagtgatg ggcgcatcgt cgcggcacgc
    12061 tgctggttca tgccgatctc acctttgaaa atctgtctca cacccggagc gcgttatgaa
    12121 aaagatcctg acaaccccga tcaaagctga agatctgcaa gatattcgcg tcggcgatgt
    12181 gatctacctg accggtacgc tggtgacctg ccgcgacgtt tgtcaccgcc gtttgatcga
    12241 actgaaacgt ccgatccctt acgatctcaa cggcaaagcg attttccacg ctggccccat
    12301 cgtgcgcaaa aacggcgaca aatgggagat ggtctccgtc ggcccgacaa ccagtatgcg
    12361 tatggaaagt tttgaacgtg aatttattga gcagaccggc gtaaaactgg tggttggcaa
    12421 aggtggtatg gggccgctga ccgaagaagg ctgccagaaa ttcaaggcgt tacatgtgat
    12481 tttcccagca ggctgcgcgg tgctggcggc aacccaggta gaagagattg aagaagtgca
    12541 ctggacagaa ctcgggatgc cggagtcact gtgggtctgc cgggtcaaag agttcggccc
    12601 gctgattgtc tctattgata cccacggcaa caacctgata gccgaaaaca aaaagctgtt
    12661 cgccgaacgc cgcgatccca tcgtggaaga gatctgcgag cacgtccatt acatcaaata
    12721 accctcccgg agaggctcac ccctctcctt tttcgcaggc ataacacgat gaaaccttcc
    12781 actgaatggt ggcgatatct tgcgccgctg gcggtcatcg ccattattgc tctacttccg
    12841 gttcccgcag ggctggagaa ccatacctgg ctctactttg ccgtttttac tggcgtgatc
    12901 gttggactga tcctcgaacc cgtgccgggt gccgtggtgg cgatggtggg tatctccatc
    12961 atcgccatac tctctccctg gctgctgttc agccccgaac agctcgccca gccaggcttt
    13021 aagttcaccg ccaaatccct ctcctgggca gtttctggtt tttctaattc ggttatctgg
    13081 ctcattttcg ccgcctttat gtttggcaca ggctatgaaa aaaccgggct tggacgccgc
    13141 attgcgctga ttctggtgaa aaagatgggg catcgcacgc tgtttctcgg ctatgcggtg
    13201 atgttctccg agctgatcct ggcacctgta acaccgtcca actcggcgcg tggtgcgggt
    13261 attatctatc ccatcatccg taacctgcca ccgctctatc aatcacaacc aaacgacagc
    13321 agttcgcgca gcattggctc gtacatcatg tggatgggga ttgttgccga ctgcgtgacc
    13381 agcgccattt tcctgacggc gatggcacct aacttgctgt taattggact gatgaaaagc
    13441 gcatctcacg ccacgctgag ttggggcgac tggttcctcg ggatgttgcc gctcagcatt
    13501 ttactggttc tgctggttcc ctggctggct tacgtgctgt acccgccggt actgaagtct
    13561 ggtgatcagg tgccgcgctg ggcagagacg gaactgcagg caatgggccc gctctgttcg
    13621 cgtgaaaaac ggatgctggg gctgatggta ggcgcgctgg tgctgtggat tttcggcggt
    13681 gattatatcg atgccgcgat ggtcggttac agcgtggtgg cgctgatgct gcttctgcgc
    13741 attatcagtt gggacgacat tgtcagtaat aaagcggcat ggaacgtttt cttctggctg
    13801 gcctcgctta tcaccctcgc gaccggactc aacaataccg gttttattag ctggtttggc
    13861 aaactgttag caggcagctt aagtggttat tcgccgacga tggtgatggt gacgttgatt
    13921 gtggtgtttt atctactgcg ctactttttc gccagcgcca cggcgtatac ctgcgccctc
    13981 gcaccgatga tgatcgccgc cgcgctggcg atgccggaaa tcccgctgcc ggtgttctgc
    14041 ctgatggttg gcgcggcaat tggtctgggg agcattctta caccatacgc caccggaccc
    14101 agtccgattt actacggtag tggttatctg ccaacggcgg attactggcg actgggggcg
    14161 atttttgggc tgatattcct cgtgttgctg gtgattaccg gcttactgtg gatgcccgtg
    14221 gtgttgcttt aagtgaggac ggtgccggaa attgctctcc ggcacccaat agttttacgc
    14281 agccggtaac tccgccagcg gccagcgcgg acgcacgcta acgccgagat ccgccgtcgc
    14341 gcctgcttta aaccgcacca ttccggcata ggcgatcatc gcgccgttat cagtacaaaa
    14401 ttccggacgc gcgtagaaca cttcgccgcg gcgttttttc atcatttcag ccagcttcgc
    14461 ccgtaacgtg cggttagcac tcacgccgcc cgccatgacc agtcgcttaa agcccgtctg
    14521 atccaacgct cgcttacact taatcatcaa cgtatcgacc accgcatctt caaaggcgcg
    14581 ggcgatatca gcacgcgtct ggtcgtcggt gccgttgtca cgaatcgtgt tcgccgcaaa
    14641 ggttttcaga ccagaaaagc tgaaatccag ccccggacgg tcggtcatcg gacgcgggaa
    14701 aacaaagcgc ccggcagtac cctgagccgc cattttcgac agtaacggtc cgccaggata
    14761 atccagcccc agcagcttcg cggttttatc aaacgcttca ccggcggcat catcgataga
    14821 ctcgccgagc agctcgtact gaccaatgcc agtcacgctg attaactgcg tatgaccgcc
    14881 ggacaccagc agcgcgacaa acggaaattc cggcgggtta tcttccagca tcggcgctaa
    14941 cagatgccct tccatatggt gtaccgggat tgccggaacg ttccaggcaa acgccagaga
    15001 acgccccacg gttgcgccaa ccagcagtgc gccgactaat ccagggcctg cggtataggc
    15061 cacagcatca atgtcttttg ccgttaaacc agactccttt agcgccgcct ggatcaacgg
    15121 tacggtttta cgcacatgat cgcgggaggc cagttcaggc acgacgccgc cgtagtcagc
    15181 gtgcaatttc acctgactat acaattggtt ggctaacaaa cctttttcat cgtcgtaaat
    15241 ggcgatgccg gtttcatcgc aggaagtttc aatacccagt acacgcatga cttgttttac
    15301 ctcgctttat taccgcgcag tgtaggacca atgcgggttg atgtaaaact ttgttcgccc
    15361 ctggagaaag cctcgtgtat actcctcacc cttataaaag tccctttcaa aaaaggccgc
    15421 ggtgctttac aaagcagcag caattgcagt aaaattccgc accattttga aataagctgg
    15481 cgttgatgcc agcggcaaac cgaattaatc aaaggtgaga ggcacatgcc ggtaattaaa
    15541 gtacgtgaaa acgagccgtt cgacgtagct ctgcgtcgct tcaagcgttc ctgcgaaaaa
    15601 gcaggtgttc tggcggaagt tcgtcgtcgt gagttctatg aaaaaccgac taccgaacgt
    15661 aagcgcgcta aagcttctgc agtgaaacgt cacgcgaaga aactggctcg cgaaaacgca
    15721 cgccgcactc gtctgtacta attccccgag agcgttgctc tccgatcaga ccgagttgta
    15781 gttgtaaggc cgtgcttccg aaaggaatgc gcggcttatt ttcgtttatg aattgctaaa
    15841 aatcggggcc tatggctgga cgaatcccac gcgtattcat taatgatctg ctggcacgca
    15901 ctgacatcgt cgatctgatc gatgcccgtg tgaagctgaa aaagcagggc aagaatttcc
    15961 acgcgtgttg tccattccac aacgagaaaa ccccgtcatt caccgttaac ggtgagaaac
    16021 agttttacca ctgctttgga tgtggcgcgc acggcaacgc gatcgacttc ctgatgaact
    16081 acgacaagct cgagttcgtc gaaacggtcg aagagctggc agcaatgcac aatcttgaag
    16141 tgccatttga agcaggcagc ggccccagcc agatcgagcg ccatcagagg caaacgcttt
    16201 atcagttgat ggacggtctg aatacgtttt accaacaatc tttacaacaa cctgttgcca
    16261 cgtctgcgcg ccagtatctg gaaaaacgcg gattaagcca cgaggttatc gctcgctttg
    16321 cgattggttt tgcgcccccc ggctgggaca acgtcctgaa gcggtttggc ggcaatccag
    16381 aaaatcgcca gtcattgatt gatgcgggga tgttggtcac taacgatcag ggacgcagtt
    16441 acgatcgttt ccgcgaacgg gtgatgttcc ccattcgcga taaacgcggt cgggtgattg
    16501 gttttggcgg gcgcgtgctg ggcaacgata cccccaaata cctgaactcg ccggaaacag
    16561 acattttcca taaaggccgc cagctttacg gtctttatga agcgcagcag gataacgctg
    16621 aacccaatcg tctgcttgtg gtcgaaggct atatggacgt ggtggcgctg gcgcaatacg
    16681 gcattaatta cgccgttgcg tcgttaggta cgtcaaccac cgccgatcac atacaactgt
    16741 tgttccgcgc gaccaacaat gtcatttgct gttatgacgg cgaccgtgca ggccgcgatg
    16801 ccgcatggcg agcgctggaa acggcgctgc cttacatgac agacggccgt cagctacgct
    16861 ttatgttttt gccggatggc gaagaccctg acacgctggt acgaaaagaa ggtaaagaag
    16921 cgtttgaagc gcggatggag caggcgatgc cactctccgc atttctgttt aacagtctga
    16981 tgccgcaagt tgatctgagt acccctgacg ggcgcgcacg tttgagtacg ctggcactgc
    17041 cattgatatc gcaagtgccg ggcgaaacgc tgcgaatata tcttcgtcag gaattaggca
    17101 acaaattagg catacttgat gacagccagc ttgaacgatt aatgccaaaa gcggcagaga
    17161 gcggcgtttc tcgccctgtt ccgcagctaa aacgcacgac catgcgtata cttatagggt
    17221 tgctggtgca aaatccagaa ttagcgacgt tggtcccgcc gcttgagaat ctggatgaaa
    17281 ataagctccc tggacttggc ttattcagag aactggtcaa cacttgtctc tcccagccag
    17341 gtctgaccac cgggcaactt ttagagcact atcgtggtac aaataatgct gccacccttg
    17401 aaaaactgtc gatgtgggac gatatagcag ataagaatat tgctgagcaa accttcaccg
    17461 actcactcaa ccatatgttt gattcgctgc ttgaactgcg ccaggaagag ttaatcgctc
    17521 gtgagcgcac gcatggttta agcaacgaag aacgcctgga gctctggaca ttaaaccagg
    17581 agctggcgaa aaagtgattt aacggcttaa gtgccgaaga gcgatcggga agcccccgac
    17641 agccgcactg agaggcagcg gcaaatatat aagtacgccc tcgtaattat cgttggcggt
    17701 aaacaaccgt tggatttcag cgttaacggc tgaaggacat cgggtcaatc gcccaacacc
    17761 aacctcatga aataagtgtg gataccgtct tatggagcaa aacccgcagt cacagctgaa
    17821 acttcttgtc acccgtggta aggagcaagg ctatctgacc tatgccgagg tcaatgacca
    17881 tctgccggaa gatatcgtcg attcagatca gatcgaagac atcatccaaa tgatcaacga
    17941 catgggcatt caggtgatgg aagaagcacc ggatgccgat gatctgatgc tggctgaaaa
    18001 caccgcggac gaagatgctg ccgaagccgc cgcgcaggtg ctttccagcg tggaatctga
    18061 aatcgggcgc acgactgacc cggtacgcat gtacatgcgt gaaatgggca ccgttgaact
    18121 gttgacccgc gaaggcgaaa ttgacatcgc taagcgtatt gaagacggga tcaaccaggt
    18181 tcaatgctcc gttgctgaat atccggaagc gatcacctat ctgctggaac agtacgatcg
    18241 tgttgaagca gaagaagcgc gtctgtccga tctgatcacc ggctttgttg acccgaacgc
    18301 agaagaagat ctggcaccta ccgccactca cgtcggttct gagctttccc aggaagatct
    18361 ggacgatgac gaagatgaag acgaagaaga tggcgatgac gacagcgccg atgatgacaa
    18421 cagcatcgac ccggaactgg ctcgcgaaaa atttgcggaa ctacgcgctc agtacgttgt
    18481 aacgcgtgac accatcaaag cgaaaggtcg cagtcacgct gccgctcagg aagagatcct
    18541 gaaactgtct gaagtattca aacagttccg cctggtgccg aagcagtttg actacctggt
    18601 caacagcatg cgcgtcatga tggaccgcgt tcgtacgcaa gaacgtctga tcatgaagct
    18661 ctgcgttgag cagtgcaaaa tgccgaagaa aaacttcatt accctgttta ccggcaacga
    18721 aaccagcgat acctggttca acgcggcaat tgcgatgaac aagccgtggt cggaaaaact
    18781 gcacgatgtc tctgaagaag tgcaccgcgc cctgcaaaaa ctgcagcaga ttgaagaaga
    18841 aaccggcctg accatcgagc aggttaaaga tatcaaccgt cgtatgtcca tcggtgaagc
    18901 gaaagcccgc cgtgcgaaga aagagatggt tgaagcgaac ttacgtctgg ttatttctat
    18961 cgccaagaaa tacaccaacc gtggcttgca gttccttgac ctgattcagg aaggtaacat
    19021 cggcctgatg aaagcggttg ataaattcga ataccgccgt ggttacaagt tctccaccta
    19081 cgcaacctgg tggatccgtc aggcgatcac ccgttctatc gcggatcagg cgcgcaccat
    19141 ccgtattccg gtgcatatga ttgagactat caacaaactc aaccgtattt ctcgccagat
    19201 gctgcaagag atgggccgtg aaccgacgcc ggaagaactg gctgaacgta tgctgatgc
//
LOCUS       DAJTRC010000075        19152 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.075, whole genome shotgun sequence.
ACCESSION   DAJTRC010000075 DAJTRC010000000
VERSION     DAJTRC010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 19152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.075"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..1950
                     /gene="pbpC"
                     /locus_tag="OO357_002823"
     CDS             <1..1950
                     /gene="pbpC"
                     /locus_tag="OO357_002823"
                     /EC_number="2.4.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417014.1"
                     /note="penicillin-binding protein 1C; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan glycosyltransferase PbpC"
                     /protein_id="HCR5887708.1"
                     /translation="QVARLLDPHPKTFGGKILQLWRALQLEWHLSKREILILYLNRAP
                     FGGTLQGIGAASWAYLGKSPANLSYSEAAMLAVLPQVPSRLRPDRWPERAEAARNKVL
                     ERMAAQGVWSREQVKESREEPIWLAPRQMPQLAPLFSRMMLGKSKSDKIVTTLDAGLQ
                     RRLEELAQNWKGRLPPRSSLAMIVVNHTDMRVRGWVGSVDLNDDSRFGHVDMVNAIRS
                     PGSVLKPFVYGLALDEGLIHPASLLQDVPRRTGDYRPGNFDSGFHGPINMSEALVRSL
                     NLPAVQVLEAYGPKRFAAKLRNVGLPLYLPNGAAPNLSLILGGAGAKLEDMAAAYTAF
                     ARHGKAGKLRLQPDDPLLERPLMSSGAAWIIRRIMADEAQPLPDSALPRVAPLAWKTG
                     TSYGYRDAWAIGVNARYVIGIWTGRPDGTPVVGQFGFASAVPLLNQVNNILLSRSANL
                     PEDPRPNSVTRGVICWPGGQSLPEGDGNCRRRLATWLLDGSQPPTLLLSEQEGINGIR
                     FPIWLDDKGQRVAADCPQAKQEMVNVWPLPLEPWLPASERRAVRLPPASTICPPYGHD
                     AQLPLQLTGVRDGAIIKRLPGAAEATLPLQSSGGAGERWWFLNGEPLTERGRNVTLHL
                     TDKGDYQLLVMDDVGQIATVKFVMQ"
     gene            2100..2531
                     /gene="ndk"
                     /locus_tag="OO357_002824"
     CDS             2100..2531
                     /gene="ndk"
                     /locus_tag="OO357_002824"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HCR5887709.1"
                     /translation="MAIERTFSIIKPNAVAKNVIGNIFARFEAAGFKIVGTKMLHLTV
                     EQARGFYAEHDGKPFFDGLVEFMTSGPIVVSVLEGENAVQRHRDLLGATNPANALAGT
                     LRADYADSLTENGTHGSDSVESAAREIAYFFGEGEVCPRTR"
     gene            2681..3835
                     /gene="trmG/rlmN"
                     /locus_tag="OO357_002825"
     CDS             2681..3835
                     /gene="trmG/rlmN"
                     /locus_tag="OO357_002825"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional tRNA
                     (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA
                     large subunit methyltransferase RlmN"
                     /protein_id="HCR5887710.1"
                     /translation="MSEQLVTPENVTTKDGKINLLDLNRQQMREFFKDLGEKTFRADQ
                     VMKWMYHYCCDNFDEMTDINKVLRGKLKEVAEIRAPEVVEEQRSSDGTIKWAIAVGDQ
                     RVETVYIPEDDRATLCVSSQVGCALECKFCSTAQQGFNRNLRVSEIIGQVWRAAKIVG
                     AAKVTGQRPITNVVMMGMGEPLLNLNNVVPAMEIMLDDFGFGLSKRRVTLSTSGVVPA
                     LDKLGDMIDVALAISLHAPNDEIRDEIVPINKKYNIETFLAAVRRYLEKSNANQGRVT
                     IEYVMLDHVNDGTEHAHQLAELLKDTPCKINLIPWNPFPDAPYGRSSNSRIDRFSKVL
                     MSYGFTTIVRKTRGDDIDAACGQLAGDVIDRTKRTLRKRMQGEAIDIKAV"
     gene            4120..5133
                     /gene="rodZ"
                     /locus_tag="OO357_002826"
     CDS             4120..5133
                     /gene="rodZ"
                     /locus_tag="OO357_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytoskeleton protein RodZ"
                     /protein_id="HCR5887711.1"
                     /translation="MNTEATHDQNEALTTGARLRNAREQLGLSQQAVAERLCLKVSTV
                     RDIEEDKAPADLASTFLRGYIRSYARLVHIPEEELLPGLEKQAPLRAAKVAPMQSFSL
                     GKRRKKRDGWLMTFTWLVLFVVIGLSGAWWWQDHKAQQEEITTMADQSSAELSSNSEQ
                     GQSVPLNTSTTTDPATTSTPPASVDTTATNTQTPAVTAPAPAVDPQQNAVVSPSQANV
                     DTAATPVPTAATTPDGAAPLPTDQAGVTTPAADPNALVMNFTADCWLEVTDATGKKLF
                     SGMQRKDGNLNLTGQAPYKLKIGAPAAVQIQYQGKPVDLSRFIRTNQVARLTLNAEQS
                     PAQ"
     gene            5160..6287
                     /gene="ispG"
                     /locus_tag="OO357_002827"
                     /gene_synonym="gcpE"
     CDS             5160..6287
                     /gene="ispG"
                     /locus_tag="OO357_002827"
                     /gene_synonym="gcpE"
                     /EC_number="1.17.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HCR5887712.1"
                     /translation="MHNQAPIQRRKSTRIYVGNVPIGDGAPIAVQSMTNTRTTDVEAT
                     VNQIKALERVGADIVRVSVPTMDAAEAFKLIKQRVNVPLVADIHFDYRIALKVAEYGV
                     DCLRINPGNIGNEERIRMVVDCARDKNIPIRIGVNAGSLEKDLQEKYGEPTPQALLES
                     AMRHVDHLDRLNFDQFKVSVKASDVFLAVESYRLLAKQIDQPLHLGITEAGGARSGAV
                     KSAIGLGLLLSEGIGDTLRVSLAADPVEEIKVGFDILKSLRIRSRGINFIACPTCSRQ
                     EFDVIGTVNALEQRLEDIITPMDVSIIGCVVNGPGEALVSTLGVTGGNKKSGLYEDGV
                     RKDRLDNNDMIDQLEARIRAKASQLDEARRIDVQQVGKIIT"
     gene            6390..7664
                     /gene="hisS"
                     /locus_tag="OO357_002828"
     CDS             6390..7664
                     /gene="hisS"
                     /locus_tag="OO357_002828"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HCR5887713.1"
                     /translation="MAKNIQAIRGMNDYLPGETAIWQRIEGTLKNVLGSYGYSEIRLP
                     IVEQTPLFKRAIGEVTDVVEKEMYTFEDRNGDSLTLRPEGTAGCVRAGIEHGLLYNQE
                     QRLWYIGPMFRHERPQKGRYRQFHQLGCEVFGLQGPDIDAELIMLTARWWRALGIFEH
                     VTLELNSIGSLEARANYRDALVAFLEQHKEKLDEDCKRRMYTNPLRVLDSKNPEVQAL
                     LNDAPALGDYLDEESREHFAGLCKLLESAGIAYTVNQRLVRGLDYYNRTVFEWVTNSL
                     GSQGTVCAGGRYDGLVEQLGGRATPAVGFAMGLERLVLLVQAVNPEFKADPVVDIYLV
                     ASGADTQSAAMALAERLRDELLGVKLMTNHGGGNFKKQFARADKWGARVAVVLGESEV
                     ANGTAVVKDLRSGEQTAVAQDSVAAHLRTLLG"
     gene            7682..8302
                     /gene="yfgM"
                     /locus_tag="OO357_002829"
     CDS             7682..8302
                     /gene="yfgM"
                     /locus_tag="OO357_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ancillary SecYEG translocon subunit YfgM"
                     /protein_id="HCR5887714.1"
                     /translation="MEIYENENDQVEAVKRFFAENGKALAVGGILGVGALTGWRYWNS
                     HQVDSARSASLAYQNAVTAVSEGKPDSIPAAEKFAAENKNTYGALASLELAQQFVDKN
                     ELEKAAAQLQQGLADTSDENLKAVINLRLARVQVQLKQADAALKTLDTIKGEGWAAIV
                     ADLRGEALLSKGDKQGARSAWEAGVKSDVTPALSEMMQMKINNLSI"
     gene            8313..9491
                     /gene="bamB"
                     /locus_tag="OO357_002830"
     CDS             8313..9491
                     /gene="bamB"
                     /locus_tag="OO357_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB"
                     /protein_id="HCR5887715.1"
                     /translation="MQLRKLLLPGLLSVTLLSGCSLFNSEEDVVKMSPLPTVENQFTP
                     TTAWSTSVGSGIGNFYSNLHPALADNVVYAADRAGLVKALNADDGKEIWSVSLAEKDG
                     WFSKEPALLSGGVTVSGGHVYIGSEKAQVYALNTSDGTVAWQTKVAGEALSRPVVSDG
                     LVLIHTSNGQLQALNEADGAVKWTVNLDMPSLSLRGESAPATAFGAAVVGGDNGRVSA
                     VLMEQGQMIWQQRISQATGSTEIDRLSDVDTTPVVVNGVVFALAYNGNLTALDLRSGQ
                     IMWKRELGSVNDFIVDGNRIYLVDQNDRVMALTIDGGVTLWAQSDLLHRLLTSPVLYN
                     GNLVVGDSEGYLHWINVEDGRFVAQQKVDSSGFQTEPVAADGKLLIQAKDGTVYSITR
                     "
     gene            9609..11081
                     /gene="der"
                     /locus_tag="OO357_002831"
     CDS             9609..11081
                     /gene="der"
                     /locus_tag="OO357_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HCR5887716.1"
                     /translation="MVPVVALVGRPNVGKSTLFNRLTRTRDALVADFPGLTRDRKYGR
                     AEIEGREFICIDTGGIDGTEDGVETRMAEQSLLAIEEADVVLFMVDARAGLMPADEAI
                     AKHLRSREKPTFLVVNKTDGLDPDQAVVDFYSLGLGEIYPIAASHGRGVLSLLEHVLL
                     PWMEDLAPQEEVDEDAEYWAQFEAEENGEEEEEDDFDPQSLPIKLAIVGRPNVGKSTL
                     TNRILGEERVVVYDMPGTTRDSIYIPMERDGREYVLIDTAGVRKRGKITDAVEKFSVI
                     KTLQAIEDANVVMLVIDAREGISDQDLSLLGFILNSGRSLVIVVNKWDGLSQEVKEQV
                     KETLDFRLGFIDFARVHFISALHGSGVGNLFESVREAYDSSTRRVGTSMLTRIMTMAV
                     EDHQPPLVRGRRVKLKYAHAGGYNPPIVVIHGNQVKDLPDSYKRYLMNYFRKSLDVMG
                     SPIRIQFKEGENPYANKRNTLTPTQMRKRKRLMKHIKKNK"
     gene            11151..11366
                     /locus_tag="OO357_002832"
     CDS             11151..11366
                     /locus_tag="OO357_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417005.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HCR5887717.1"
                     /translation="MELHCPKCQHVLDQDNGHARCPSCGEFIEMKALCPDCHQPLQVL
                     KACGAVDYFCQHGHGLISKKRVEFVLA"
     gene            complement(11363..12733)
                     /gene="xseA"
                     /locus_tag="OO357_002833"
     CDS             complement(11363..12733)
                     /gene="xseA"
                     /locus_tag="OO357_002833"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708348.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HCR5887718.1"
                     /translation="MLPSQSPAIFTVSRLNQTVRLLLEHEMGQVWISGEISNFTQPAS
                     GHWYFTLKDDTAQVRCAMFRNSNRRVTFRPQHGQQVLVRANITLYEPRGDYQIIVESM
                     QPAGEGLLQQKYEQLKAKLQAECLFDQQYKKPLPSPAHCVGVITSKTGAALHDILHVL
                     KRRDPSLPVIIYPTSVQGDDAPGQIVRAIELANQRNECDVLIVGRGGGSLEDLWSFND
                     ERVARAIFASRIPVVSAVGHETDVTIADFVADLRAPTPSAAAEVVSRNQQELLRQVQS
                     ARQRLEMAMDYYLANRTRRFTQIHHRLQQQHPQLRLARQQTMLERLQKRMSFALENQL
                     KRAGQQQQRLTQRLNQQNPQPKIHRTQTRIQQLEYRLAEILRAQLSATRERFGNAVTH
                     LEAVSPLSTLARGYSVTTATDGNVLKKVKQVKTGEMLTTRLEDGWIESEVKNIQPVKK
                     SRKKVH"
     gene            12895..14361
                     /gene="guaB"
                     /locus_tag="OO357_002834"
     CDS             12895..14361
                     /gene="guaB"
                     /locus_tag="OO357_002834"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004151980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HCR5887719.1"
                     /translation="MLRIAKEALTFDDVLLVPAHSTVLPNTADLSTQLTKTIRLNIPM
                     LSAAMDTVTEARLAIALAQEGGIGFIHKNMSIERQAEEVRRVKKHESGVVTDPQTVLP
                     TTTLREVKELTERNGFAGYPVVTEENELVGIITGRDVRFVTDLNQPVSVYMTPKERLV
                     TVREGEAREVVLAKMHEKRVEKALVVDDEFHLIGMITVKDFQKAERKPNACKDEQGRL
                     RVGAAVGAGAGNEERVDALVAAGVDVLLIDSSHGHSEGVLQRIRETRAKYPDLQIIGG
                     NVATAAGARALAEAGCSAVKVGIGPGSICTTRIVTGVGVPQITAVADAVEALEGTGIP
                     VIADGGIRFSGDIAKAIAAGASAVMVGSMLAGTEESPGEIELYQGRSYKSYRGMGSLG
                     AMSKGSSDRYFQSDNAADKLVPEGIEGRVAYKGRLKEIIHQQMGGLRSCMGLTGCGTI
                     DELRTKAEFVRISGAGIQESHVHDVTITKESPNYRLGS"
     gene            14430..16007
                     /gene="guaA"
                     /locus_tag="OO357_002835"
     CDS             14430..16007
                     /gene="guaA"
                     /locus_tag="OO357_002835"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005130181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HCR5887720.1"
                     /translation="MTENIHKHRILILDFGSQYTQLVARRVRELGVYCELWAWDVTEA
                     QIRDFNPSGIILSGGPESTTEENSPRAPQYVFEAGVPIFGVCYGMQTMAMQLGGHVEA
                     SNEREFGYAQVEVVNDSALVRGIEDALTADGKPLLDVWMSHGDKVTAIPSDFVTVAST
                     ESCPFAIMANEEKRFYGVQFHPEVTHTRQGMRMLERFVRDICQCEALWTPAKIIDDAV
                     ARIREQVGDDKVILGLSGGVDSSVTAMLLHRAIGKNLTCVFVDNGLLRLNEAEQVLDM
                     FGDHFGLNIVHVPAEDRFLSALAGENDPEAKRKIIGRVFVEVFDEEALKLEDVKWLAQ
                     GTIYPDVIESAASATGKAHVIKSHHNVGGLPKEMKMGLVEPLKELFKDEVRKIGLELG
                     LPYDMLYRHPFPGPGLGVRVLGEVKKEYCDLLRRADAIFIEELRKADLYDKVSQAFTV
                     FLPVRSVGVMGDGRKYDWVVSLRAVETIDFMTAHWAHLPYDFLGRVSNRIINEVNGIS
                     RVVYDISGKPPATIEWE"
     gene            complement(16102..16641)
                     /locus_tag="OO357_002836"
     CDS             complement(16102..16641)
                     /locus_tag="OO357_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5384 family protein"
                     /protein_id="HCR5887721.1"
                     /translation="MKKVFLCAILASLSYPAIASSLQDQLSAVAEAEQQGKNEEQRQH
                     DEWVAERNREIQQEKQRRANAQAAANKRAATAAANKKARQDKLDAEATADKKRDQSYE
                     DELRSLEIQKQKLALAKEEARVKRENEFIDQELKHKAAQTDVVQSEADANRNMTEGGR
                     DLMKSVGKAEENKSDSWFN"
     gene            complement(16657..17175)
                     /locus_tag="OO357_002837"
     CDS             complement(16657..17175)
                     /locus_tag="OO357_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper 2TM domain-containing protein"
                     /protein_id="HCR5887722.1"
                     /translation="MMKFKKCLLPVAMLASFTLAGCQSNADDHAADVYQTDQLNTKQE
                     TKTVNIISILPAKVAVDNSQNKRNAQAFGALIGAVAGGVIGHNVGSGSNSGTTAGAVG
                     GGAVGAAAGSMVNDKTLVEGVSLTYKEGTKVYTSTQEGKECQFTTGLAVVITTTYNET
                     RIQPNTKCPEKS"
     gene            complement(17486..17677)
                     /locus_tag="OO357_002838"
     CDS             complement(17486..17677)
                     /locus_tag="OO357_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2633 family protein"
                     /protein_id="HCR5887723.1"
                     /translation="MSQATSMRKRHRFNSRMTRIVLLISFIFFFGRFIYSSVGAWQHH
                     QSKKEAQQSTLSVESPVQR"
     gene            17695..17847
                     /locus_tag="OO357_002839"
     CDS             17695..17847
                     /locus_tag="OO357_002839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887724.1"
                     /translation="MSHAVDGTYIKAQQDKMVNSSLCKKASVDTYFNVMEVNLIIYND
                     DVIMMN"
     gene            18029..>19152
                     /gene="pdeF"
                     /locus_tag="OO357_002840"
     CDS             18029..>19152
                     /gene="pdeF"
                     /locus_tag="OO357_002840"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5887725.1"
                     /translation="MKLNATYIKIRDKWWGLPLFLPSLILPIFAHINTFAHISSGEVF
                     LFYLPLALMISMMMFFSWAALPGITLGIFVRKYAELGFYETLSLTANFIIIIILCWGG
                     YRVFTPRRNNVSHGDTRLISQRIFWQIVFPATLFLILFQFAAFVGLLASRENLVGVMP
                     FNLGTLINYQALLVGNLIGVPLCYFIIRVVRNPFYLRSYYSQLKQQVDAKVTKKEFAL
                     WLLALGALLLLLCMPLNEKSTIFSTNYTLSLLLPLMMWGAMRYGYKLISLLWAVVLMI
                     SIHSYQNYIPIYPGYTTQLTITSSSYLVFSFIVNYMAVLATRQRAVVRRIQRLAYVDP
                     VVHLPNVRALNRALRDAPWSALCYLRIPGMEMLVKNYGIML"
BASE COUNT         4513 a         4663 c         5203 g         4773 t
ORIGIN      
        1 caggttgctc gtctgcttga tcctcacccc aaaacatttg gtggcaaaat tctccagctc
       61 tggcgcgcgt tgcaactgga gtggcatttg tctaagcgtg aaattctgat cttgtatctt
      121 aaccgcgctc cgtttggcgg tacgttgcag gggatcggtg cggcaagttg ggcttatctc
      181 ggaaaatcgc ctgcgaattt aagctattcc gaggcggcaa tgctggcggt gttaccgcaa
      241 gtgcccagcc gactgcgccc ggatcgttgg ccggagcgtg ccgaagccgc gcgtaataaa
      301 gtgctcgaac ggatggctgc gcaaggtgtg tggtcccgtg agcaggtaaa agagtcaagg
      361 gaagaaccca tctggctggc accccgacaa atgccgcaac tggcaccgct gttttcgcgc
      421 atgatgctcg gtaaaagcaa aagcgacaaa atcgttacta cgctggatgc cggtcttcaa
      481 cgacgtctgg aagaactggc gcaaaactgg aaagggcggt tgccaccgcg cagctcactg
      541 gcgatgatcg tggttaatca taccgatatg cgtgttcgcg gctgggtggg atcggttgat
      601 ctcaacgatg attcacgctt tggtcatgtt gatatggtca atgcgatccg atcgccagga
      661 tcggtgctca aaccgtttgt ttatggtctg gcgctggatg aaggcttgat ccacccggca
      721 tcactgctgc aagacgtccc ccggcgcacc ggtgattatc gaccaggtaa ctttgatagc
      781 ggttttcatg gcccgatcaa catgagcgag gcgctggtgc gctcgctgaa cttacctgct
      841 gtgcaggtgc tggaagccta tggaccgaaa cggtttgcgg caaagttacg caatgttgga
      901 ttgccgttat atttgcccaa cggcgctgcg ccgaatcttt cactcattct cggcggcgct
      961 ggtgcaaaac tggaagatat ggcggcagcg tataccgcgt ttgctcgcca cggcaaggca
     1021 ggcaaattgc gcttacagcc tgacgatccg ctgcttgaac ggcctttaat gtcgtcgggg
     1081 gcggcgtgga tcattcgccg gattatggct gatgaagcgc aacccttgcc ggatagtgcc
     1141 ttgccgcgcg tcgccccact ggcatggaaa acgggcacca gctatggcta tcgtgacgcc
     1201 tgggcgattg gggttaacgc tcgctatgtc attgggatct ggactggcag acctgacggc
     1261 acgcccgttg ttggtcagtt tggctttgcc agtgccgtac cattgttgaa tcaggtcaat
     1321 aatatcttac tgtcgcgcag tgcgaatctg ccggaagacc cgcgcccgaa ctcagtcact
     1381 cgtggcgtta tctgctggcc gggcggacag tctttgccgg aaggtgacgg taactgtcgc
     1441 cgccgcctgg caacctggct gctggatggc agccagccgc caacgctgtt actgtcagag
     1501 caggaaggga ttaatggcat tcgcttcccc atctggctgg atgacaaagg gcagcgcgtc
     1561 gccgctgact gcccgcaggc gaagcaagaa atggtaaatg tctggccgct accgctggaa
     1621 ccctggctgc ccgcatcaga acgtcgcgct gtgcgcttac caccggcctc gacaatttgc
     1681 ccgccatacg gtcacgatgc ccagctccct ctgcagctga caggcgttcg cgatggcgcg
     1741 attattaaac gtttaccggg cgcagcggaa gcgactttgc cgttgcaatc aagtggaggg
     1801 gcaggtgaac gctggtggtt tctgaatggc gaaccgttaa ctgaacgcgg gcgcaacgtg
     1861 accctgcatt tgacggataa aggtgattat caattgctgg tgatggatga tgtggggcaa
     1921 atcgcgacag tgaaatttgt catgcaatag tctgttttgt ccctgattgt tgctaaaact
     1981 cattttattt taaaaaaaat gttaccttca tcaatagtca acggccctgt tgctcattat
     2041 aatccgcgcc atctcgtacg ctggtacaga caacaacaga acaatttaca gaggtaaaaa
     2101 tggctattga acgtactttt tccatcatca aaccgaacgc ggtagcaaaa aacgtcattg
     2161 gtaatatctt tgcgcgcttt gaagctgcag ggttcaaaat tgtcggcacc aaaatgctgc
     2221 atctgaccgt tgaacaggca cgtggctttt atgctgaaca cgatggaaaa ccgttctttg
     2281 atggtctggt tgaattcatg acctctggcc cgatcgtggt ttccgtgctg gaaggtgaaa
     2341 acgccgttca gcgtcaccgc gatctgctgg gcgcgaccaa tccggcaaac gcactggctg
     2401 gtactctgcg cgctgattac gctgacagcc tgaccgaaaa cggtacccac ggttctgatt
     2461 ccgtcgaatc tgccgctcgc gaaatcgctt atttcttcgg cgaaggcgaa gtgtgcccgc
     2521 gcacccgtta ataatttcgt aaatgccgcg tgcaaacgtg gcatccgtgc gccagaattt
     2581 gtacaatgca gcgcccccgg acgagcagcc gctcaccggg gcgtttcttt tttcaaccct
     2641 ccaggggcca taacgtgtaa taacgaggcc ggaatagcat atgtctgaac aattagtcac
     2701 acctgaaaac gtcaccacga aagatggaaa aatcaacctg ctggatctca accgtcagca
     2761 gatgcgggag ttttttaaag atttaggtga aaaaaccttc cgcgccgatc aggtgatgaa
     2821 gtggatgtat cactattgct gcgacaactt tgatgagatg accgacatca acaaagtgtt
     2881 gcgcggcaaa ctgaaagagg tggcggaaat ccgcgcaccg gaagtggttg aagaacagcg
     2941 ttcatccgac ggcaccatta aatgggcgat cgccgttggc gatcagcgcg tcgaaacggt
     3001 gtatatcccg gaagacgacc gtgccacgct ctgcgtctct tcgcaggtgg ggtgtgcgct
     3061 ggagtgtaaa ttctgttcca ccgcccagca gggctttaac cgcaacctgc gggtatcgga
     3121 aattattggc caggtgtggc gtgcggcgaa aatcgtcggc gcagcaaaag tcaccggtca
     3181 gcgcccaatc actaacgtcg tgatgatggg catgggcgag ccgctgctca acctgaacaa
     3241 cgtcgttccg gcgatggaaa tcatgcttga tgatttcggt tttggcctgt ctaaacgtcg
     3301 cgtgacgctt tccacttccg gtgtagttcc ggcgctggat aaactgggcg atatgatcga
     3361 cgttgcactg gcaatttccc tgcacgcgcc gaacgacgaa attcgtgacg aaattgtgcc
     3421 gatcaacaaa aagtacaata tcgaaacgtt ccttgctgcg gtgcgccgtt atctggagaa
     3481 atccaacgcc aatcagggcc gagtcactat tgaatacgtg atgcttgacc acgttaacga
     3541 cggcactgaa cacgcgcacc aactggcgga actgctgaaa gatacgccgt gtaagatcaa
     3601 cctgatcccg tggaacccgt tcccggacgc gccgtatgga cgcagttcga acagccgtat
     3661 cgaccgtttc tcaaaagtgc tgatgagcta cggtttcacc actattgtgc gtaaaacgcg
     3721 tggtgatgat atcgatgctg cctgtggtca gttggcgggc gatgttatcg accgtacgaa
     3781 acgtactctg cgtaaacgga tgcagggtga agccatcgat attaaagcgg tctgatagcc
     3841 attacgccac ggttacttta tgtaccgtgg cgtaatcttt tatgaatgaa gtattaagtt
     3901 gcaattgtct ggtcaatatt aacggtgcgt ttttgctgac tttaaggcag tatgtaacga
     3961 tgcaacagta agttagcctc agtgaatgtg ggctttgtca cgagcacaca gacggtctta
     4021 tactgtatga taacggttaa cttaacggat gtttcgcggt gtgggtgagc atgatggttc
     4081 accggcatct caattctcat ttaaacgtac ctgcagcgaa tgaatactga agccacgcac
     4141 gaccaaaatg aagcacttac taccggcgct cgcctgcgta atgctcgcga acaactagga
     4201 cttagtcagc aggccgttgc cgagcgactt tgcctgaagg tttccacggt acgcgacatt
     4261 gaagaagata aggcacccgc cgatcttgct tcaacattcc tgcgcggata tatccgctct
     4321 tatgcgcgtc tggtacatat tccagaagaa gaactgctgc cagggctgga aaagcaggct
     4381 ccacttcggg ctgcaaaagt tgcgccaatg cagagttttt ccctcggtaa acgccgcaaa
     4441 aaacgcgacg gctggctaat gaccttcacc tggctggtgt tgtttgtggt tatcggcctg
     4501 agcggtgcct ggtggtggca agaccacaaa gctcagcagg aagagatcac cactatggcc
     4561 gatcaatctt cggcggaact gagcagtaat agcgagcagg ggcagagtgt tccgttaaat
     4621 acgtcgacaa ctacagaccc ggctacaacg tccacgccgc cagcgtctgt ggatactacc
     4681 gcaaccaaca cgcaaacacc tgccgtaact gcgccagcac cagctgttga tccgcaacag
     4741 aatgcggttg tttcgccttc gcaggcaaat gttgataccg ccgcgacccc ggtaccaacg
     4801 gcagcaacaa cgccagatgg tgctgcgccg ttgccaaccg atcaggctgg cgtgaccacg
     4861 ccggcggctg atccgaatgc gctggtgatg aactttactg ccgattgctg gctggaggtc
     4921 actgatgcta ccggtaaaaa attgtttagc gggatgcagc gtaaagacgg taatttgaat
     4981 ttaaccggcc aggcaccgta taaactgaaa attggtgcgc cagccgcagt acagatccag
     5041 tatcaaggga aacctgtcga tctgagtcgt tttatcagaa ctaaccaggt tgcgcgtctg
     5101 accctcaatg ccgaacaatc accggcgcag taacagacgg gtaacgcggg agatttttca
     5161 tgcataacca ggctccaatt caacgtagaa aatcaacacg tatttacgtt gggaatgtgc
     5221 cgattggcga tggtgctccc atcgccgtac agtccatgac caatacgcgt acgacagatg
     5281 tcgaagcaac ggtcaatcaa atcaaggcgc tggaacgcgt tggcgctgat atcgtccgtg
     5341 tctccgtacc gacgatggac gcggcagaag cgttcaaact catcaaacag cgggttaacg
     5401 tgccgctggt ggctgacatc cacttcgact atcgcattgc gctgaaagta gcggaatacg
     5461 gcgtcgattg tctgcgtatt aaccctggta atatcggtaa tgaagagcgt attcgcatgg
     5521 tggttgactg tgcgcgcgat aaaaacattc cgatccgtat tggcgttaac gccggatcgc
     5581 tggaaaaaga tctgcaagaa aagtatggcg aaccgacgcc gcaggcgttg ctggaatccg
     5641 ccatgcgtca tgttgatcat ctcgatcgcc tgaacttcga tcagttcaaa gtcagcgtga
     5701 aagcgtctga cgtcttcctc gctgttgagt cttatcgttt gctggcaaaa cagatcgatc
     5761 agccgctgca tctggggatc accgaagccg gtggtgcacg cagcggggca gtaaaatccg
     5821 ccattggttt aggtctgctg ctgtcggaag gcatcggcga cacgctgcgt gtatcgctgg
     5881 cggccgatcc ggtcgaagag atcaaagtcg gtttcgatat tttgaaatcg ctgcgtatcc
     5941 gttcgcgagg gattaacttc atcgcctgcc cgacctgttc gcgtcaggaa tttgatgtta
     6001 tcggtacggt taacgcgctg gagcaacgcc tggaagatat catcactccg atggacgttt
     6061 cgattatcgg ctgcgtggtg aatggcccag gtgaggcgct ggtttctaca ctcggcgtca
     6121 ccggcggcaa caagaaaagc ggcctctatg aagatggcgt gcgcaaagac cgtctggaca
     6181 acaacgatat gatcgaccag ctggaagcac gcattcgtgc gaaagccagt cagctggacg
     6241 aagcgcgtcg aattgacgtt cagcaggttg gaaaaataat aacgtgatgg gaagcgcctc
     6301 gcttcccgtg tatgattgaa cccgcatggc tcccgaaaca ttgagggaag cgttgagggt
     6361 tcatttttat attcagaaag agaataaacg tggcaaaaaa cattcaagcc attcgcggca
     6421 tgaacgatta cctgcctggc gaaacggcca tctggcagcg cattgaaggc acactgaaaa
     6481 acgtgctcgg cagctacggt tacagtgaaa tccgcttgcc gattgtagag cagaccccgc
     6541 tattcaaacg tgcgattggt gaagtcaccg acgtggttga aaaagagatg tacacctttg
     6601 aggatcgcaa tggcgacagc ctgactctgc gccctgaagg gacggcgggc tgtgtacgcg
     6661 ccggcatcga gcatggtctt ctgtacaatc aggaacagcg tctgtggtat atcgggccga
     6721 tgttccgtca cgagcgtccg cagaaagggc gttatcgtca gttccatcag ttgggctgcg
     6781 aagttttcgg tctgcaaggt ccggatatcg acgctgaact gattatgctc actgcccgct
     6841 ggtggcgtgc gctgggtatc ttcgaacacg taactcttga gctgaattct atcggttcgc
     6901 tggaagcacg cgccaattac cgcgatgcgc tggtggcatt ccttgagcag cataaagaaa
     6961 agctggacga agactgcaaa cgccgcatgt acactaaccc gctgcgcgtg ctggattcaa
     7021 aaaatccgga agtgcaggcg cttctcaacg acgctccggc attaggtgat tatctggacg
     7081 aggaatctcg tgagcatttt gccggtctgt gcaaactgct ggagagcgcg gggatcgctt
     7141 acaccgtaaa ccagcgtctg gtgcgtggtc tggattacta taaccgtacc gttttcgagt
     7201 gggtgactaa cagtctcggc tcccagggca ccgtgtgtgc aggcggtcgt tatgacggtc
     7261 ttgtggaaca actgggcggt cgtgcaacac cggctgtcgg ttttgcgatg ggcctcgaac
     7321 gtcttgtatt gttagtacag gccgttaatc cggaatttaa agccgatcct gttgtcgata
     7381 tatacctggt ggcttcaggt gctgatacac aatctgcggc tatggcatta gctgagcgtc
     7441 tgcgtgatga attactgggc gtgaaattga tgaccaacca cggcggcggc aactttaaga
     7501 aacagtttgc ccgtgctgat aaatggggtg cccgcgttgc tgtggtgctg ggtgagtctg
     7561 aagtggctaa cggcacagca gtagtgaagg atttgcgctc tggtgagcaa acggcagttg
     7621 cgcaggatag cgtagccgcg catttgcgca cgttactggg ttaaggaagg agaaggacag
     7681 cgtggaaatt tacgagaacg aaaacgacca ggtagaagcg gttaaacgct tttttgctga
     7741 aaatggcaaa gcactggctg ttggggggat tttgggcgtt ggcgcactga ctggctggcg
     7801 ctactggaac agccatcagg ttgattctgc acgctccgct tctcttgcct atcaaaatgc
     7861 ggttaccgca gtgagcgaag gcaaaccgga tagcatcccg gcggcggaaa aatttgctgc
     7921 tgaaaataaa aatacttatg gtgcgctggc ttctttggaa cttgcgcagc aatttgttga
     7981 caaaaatgaa ctagagaaag ctgccgccca gttacaacag gggctggcag acacgagcga
     8041 tgaaaatctc aaagccgtga taaatctgcg tcttgctcgc gttcaggtac agctcaagca
     8101 ggctgatgcc gcgctgaaaa cccttgatac catcaaaggt gaagggtggg ctgccattgt
     8161 tgccgacttg cgtggtgaag cattgctgag caaaggtgat aagcaaggtg cgcgtagtgc
     8221 atgggaagca ggcgtgaaaa gcgatgttac tccggcactg agcgaaatga tgcagatgaa
     8281 aattaataat ttgtccatct gagagggacc cgatgcaatt gcgtaaatta ctgctgccag
     8341 gactgctttc cgttaccctt ttaagcggct gttcgctgtt taacagcgaa gaagatgtgg
     8401 taaagatgtc cccattgcca accgttgaaa accagtttac gccgaccacg gcgtggagca
     8461 cttccgttgg tagcggcatt ggcaacttct attccaatct tcatccggca ctggcggaca
     8521 acgttgtcta tgcagcggac cgcgctggtt tggtaaaagc gctgaatgcg gatgatggca
     8581 aagaaatctg gtctgtcagc ctggccgaga aagatggctg gttctctaaa gagcctgcat
     8641 tactttctgg cggtgtgacc gtgtctggtg ggcatgtcta cattggcagc gaaaaggcgc
     8701 aggtttacgc gctgaatacc agcgatggta ctgtggcatg gcaaactaaa gtcgcgggtg
     8761 aagcactttc gcgcccggtg gtcagcgacg gtctggtgtt aatccacacc agtaacggtc
     8821 agttacaagc gctgaacgaa gctgacggcg ctgtcaaatg gacagttaac ctcgatatgc
     8881 cttcgctctc tttgcgtggc gagtctgcgc cggcaacggc ttttggtgcg gccgtcgtgg
     8941 gtggcgataa tggtcgcgtc agcgcagtgc tgatggaaca gggccagatg atttggcagc
     9001 agcgtatttc ccaggcgacc ggttctaccg aaattgaccg tctgagcgat gttgacacga
     9061 ctcccgtcgt tgttaacggc gttgttttcg cgctggccta taatggtaac ctgacggcgc
     9121 ttgatctgcg cagtggtcag attatgtgga aacgcgaact gggttcggtg aatgatttca
     9181 tcgtcgatgg caatcgcatc tatctggtcg atcaaaatga ccgggtgatg gcgttgacca
     9241 ttgatggcgg cgttacgctg tgggcacaaa gcgatctgct gcatcgcctg ctgacttctc
     9301 cggtgctgta taatggcaac ctggtggtcg gtgacagtga aggttatctg cactggatta
     9361 acgtcgaaga tggtcgtttc gttgcccagc aaaaagttga tagttccggt ttccagactg
     9421 aaccggttgc cgctgacggc aaactgctga tccaggcaaa agacggaacc gtgtactcta
     9481 ttacacgtta atcgtctctg tcgttcactt tgaaaacggc tcctggacag gggccgtttt
     9541 cctgttttta acaacgacgc gaatatagcg tgcgttgtct gatgatttat aaaaatgagg
     9601 ctttaaacat ggtacctgtg gtcgcgcttg tcgggcgccc taacgtagga aaatccacgt
     9661 tatttaaccg tctaactcgc acccgagatg cgctggttgc ggatttcccg ggtctgactc
     9721 gtgaccgtaa gtacggtcgt gcggaaattg aaggccgtga gtttatctgt attgataccg
     9781 gcgggattga tggcacagaa gacggtgtag aaacccgcat ggcggaacag tcgctgctgg
     9841 cgattgaaga agcggacgtc gtactgttta tggtggatgc gcgcgcgggc ctgatgccgg
     9901 cagatgaagc gattgccaaa catctgcgct cccgtgaaaa accgaccttc ctggtggtaa
     9961 acaaaactga cggtctggat cccgatcagg cagtggttga tttctactcg cttggtttag
    10021 gtgaaatcta cccgatcgcc gcgtctcacg gtcgtggcgt attaagtctg ctggagcatg
    10081 tgctgctgcc gtggatggaa gatctcgcac cgcaagagga agtcgacgaa gacgctgaat
    10141 actgggcgca atttgaagcg gaagagaacg gcgaagaaga agaggaagac gacttcgacc
    10201 cgcaaagtct gccgatcaaa ctggcgattg tgggtcgtcc gaacgtaggt aagtctacac
    10261 tcactaaccg tattcttgga gaagagcgcg ttgttgttta cgacatgcct ggcacgacgc
    10321 gtgacagcat ctacatccca atggaacgcg atggacgtga gtatgtgctc attgacaccg
    10381 ctggcgtacg taaacgcggc aaaatcaccg atgctgtaga gaaattctcc gtaatcaaaa
    10441 cgttgcaggc cattgaagac gccaacgtgg tgatgttagt gattgatgcg cgcgaaggta
    10501 tttccgatca ggatctctcg ctgctgggct ttattctcaa tagtgggcgc tcacttgtca
    10561 ttgtggtgaa taagtgggat ggcctgagcc aggaagtgaa agagcaggtg aaagaaacgc
    10621 tggacttccg tctgggcttt atcgattttg ctcgtgtgca ctttatctct gccttgcacg
    10681 gcagtggtgt tggtaacttg tttgaatcag tacgtgaagc gtatgacagc tccacccgtc
    10741 gtgtggggac ctctatgctg acgcgcatca tgacgatggc tgttgaagat caccaaccgc
    10801 cgctggtacg cggtcgtcgt gtgaagctga aatatgccca cgctggtggt tataacccgc
    10861 cgattgtggt gattcacggt aatcaggtga aagacctgcc tgattcctac aagcgctact
    10921 tgatgaacta cttccgcaaa tcgctggacg taatgggatc gccgattcgt attcagttca
    10981 aagaagggga aaacccgtat gcgaacaagc gtaacaccct gacgccaacc cagatgcgta
    11041 aacgtaagcg tctgatgaag cacatcaaga aaaataaata attatccgta agtattttat
    11101 ctgccattcc ctctatgaat gtagagggaa tggctaccgg aggaattcat atggaactgc
    11161 attgtccaaa atgtcagcat gtgcttgatc aggataacgg ccatgcgcgt tgcccatcct
    11221 gcggggaatt tattgaaatg aaagctcttt gtccagactg ccatcaaccg ttgcaggtgt
    11281 taaaagcctg cggtgcagta gattacttct gtcagcacgg gcatggcttg atatcgaaaa
    11341 aacgcgttga attcgtgctg gcttaatgaa cctttttacg cgattttttt accggctgga
    11401 tgttttttac ttcactttct atccagccgt cttccagacg tgtggttagc atttcacccg
    11461 ttttaacttg cttcactttt ttcagtacat tgccgtcagt agcagtagta acgctgtatc
    11521 cacgcgccag cgttgacagt gggcttacgg cttcgaggtg cgttactgca ttaccgaaac
    11581 gttcacgcgt ggcgctaagc tgtgcgcgca ggatttctgc taaacgatat tccagttgct
    11641 gaatgcgcgt ttgcgtgcga tgaatcttcg gctgtggatt ttgctgattc agccgctgtg
    11701 ttaatcgctg ctgctgttgc ccggcacgct taagctgatt ttccagcgca aagctcatcc
    11761 gtttttgcag gcgttcaagc atggtttgct ggcgtgccag ccggagctgc ggatgctgct
    11821 gctgcaagcg atgatgaatc tgcgtaaagc gacgtgtacg gttggcgaga taataatcca
    11881 tcgccatctc cagccgttga cgggccgatt gcacctggcg cagtaactct tgctgattac
    11941 ggctcactac ttcagcggcg gcagacggcg ttggcgcacg cagatcggca acaaaatcgg
    12001 caatggtcac atccgtctca tgcccgacgg cgctgacgac cggaatgcgg ctggcaaaaa
    12061 tcgcccgcgc tacgcgttcg tcgttaaaac tccataaatc ttccagcgaa ccgccgccgc
    12121 gcccaacgat caacacgtcg cactcattgc gctgattcgc cagttcaatg gcgcgaacaa
    12181 tttgccccgg cgcgtcatcg ccctgaacgg aggtggggta gatgatcacc ggtagagaag
    12241 gatcgcgacg ttttaacaca tgcaaaatat catgtagcgc agcaccggtt tttgaggtga
    12301 tcacaccaac gcaatgcgca ggggagggaa gtggtttttt gtattgctga tcgaacaaac
    12361 attcagcctg caacttcgct ttgagctgtt cgtacttctg ttgcagcagc ccttcaccgg
    12421 ccggctgcat actctcaacg attatctggt agtcgccgcg cggctcgtag agcgtaatat
    12481 tggcgcgaac taaaacttgt tgcccatgct gcggacggaa ggtgacccgg cggttgctgt
    12541 tgcggaacat cgcgcagcgt acctgggcgg tgtcgtcttt gagtgtaaag taccagtgac
    12601 cggaagccgg ttgtgtgaaa ttagagattt cgccgctgat ccaaacctgt cccatctcat
    12661 gctcaagcag cagacgaacc gtttgattca ggcgactaac ggtaaaaatt gcaggggatt
    12721 gagaaggtaa catgtgagcg agatcaaatt ctaaatcagc aggttattca gtctatagta
    12781 acccgccctt cggggatagc aagcattttt tgcaaaaagg ggtagatgca atcggttacg
    12841 ctctgtataa tgccgcggca atatttatta accactctgg tcgagatatt gcccatgcta
    12901 cgtatcgcta aagaagctct gacgtttgac gacgttctcc tcgttcctgc tcattctacc
    12961 gttctgccga atactgctga cctcagcacc cagctgacga aaactattcg tctgaatatc
    13021 cctatgcttt ccgcagcaat ggataccgta acggaagcgc gcctggctat tgctctggct
    13081 caggaaggcg gtatcggctt tatccacaaa aacatgtcca ttgaacgcca ggcagaagaa
    13141 gttcgccgtg tgaaaaaaca cgaatctggt gtggtgactg atccgcagac tgttctgcca
    13201 accacgacgc tgcgcgaagt gaaagaactg accgagcgta acggttttgc gggctatccg
    13261 gtcgttaccg aagaaaacga actggtgggt attatcaccg gtcgtgacgt gcgttttgtt
    13321 accgacctga accagccggt tagcgtttac atgacgccga aagagcgtct ggtcaccgtg
    13381 cgtgaaggtg aagcccgtga agtggtgctg gcaaaaatgc acgaaaaacg cgttgaaaaa
    13441 gcgctggtgg ttgatgacga attccacctg atcggcatga tcaccgtgaa agacttccag
    13501 aaagcggaac gtaaaccgaa cgcttgtaaa gacgagcaag gccgtctgcg tgttggtgct
    13561 gcagttggcg caggtgcggg taacgaagag cgtgttgacg cgctggttgc cgctggcgtt
    13621 gacgttctgc tgatcgactc ctcccacggt cactcagaag gcgttttgca gcgtatccgt
    13681 gaaacccgtg ctaaatatcc ggatctgcaa atcatcggcg gcaacgtggc aacagctgca
    13741 ggtgcacgcg ctttggcaga agctggttgc agcgcggtta aagtcggtat cggccctggt
    13801 tctatctgta cgactcgtat cgttactggc gtaggtgttc cgcagatcac tgccgttgct
    13861 gacgcagttg aagccctgga aggcaccggt attccggtta tcgcagatgg tggtattcgc
    13921 ttctccggcg acatcgccaa agctatcgcc gctggcgcaa gcgcggtgat ggtaggttcc
    13981 atgctggcgg gtactgaaga atctccgggt gaaatcgaac tctaccaggg ccgttcttat
    14041 aaatcttacc gtggtatggg ttccctgggc gcgatgtcca aaggttcctc tgaccgttat
    14101 ttccagagcg ataacgctgc cgacaaactg gtgccggaag gtatcgaagg tcgcgtagct
    14161 tataaaggtc gcctgaaaga gatcattcac cagcagatgg gcggcctgcg ctcctgtatg
    14221 ggtctgaccg gctgtggtac tatcgacgaa ctgcgtacta aagcggagtt tgtacgtatc
    14281 agcggtgcgg gcattcagga aagccacgtt cacgacgtga ccattactaa agagtccccg
    14341 aactaccgtc tgggctcctg attctcttcg cccgatttca tgtcgggcga tttatattat
    14401 ctgtttcact tgcctcggaa taagcgtcaa tgacggaaaa cattcataag catcgcatcc
    14461 tcattctgga cttcggttct cagtacactc aactggttgc gcgccgcgtg cgtgagctgg
    14521 gtgtttactg cgaactgtgg gcgtgggatg tgacagaagc acaaattcgt gacttcaatc
    14581 caagcggaat tattctttcc ggcggcccgg aaagtactac tgaagaaaac agtccgcgtg
    14641 cgccgcagta tgtctttgaa gcaggcgtac cgatattcgg cgtttgctat ggcatgcaga
    14701 ccatggcaat gcagttgggc ggtcacgttg aagcctctaa cgaacgtgaa tttggctacg
    14761 cgcaggtaga agtcgtaaac gacagcgcac tggttcgcgg tatcgaagat gcgctgaccg
    14821 cagacggtaa accgctgctc gatgtctgga tgagccacgg cgataaagtt accgctatcc
    14881 cgtccgactt cgtcaccgta gccagcaccg aaagctgccc gtttgccatt atggctaacg
    14941 aagaaaaacg cttctatggc gtacagttcc acccggaagt gactcacacc cgccagggta
    15001 tgcgcatgct ggagcgtttt gtgcgtgata tctgccagtg tgaagccctg tggacgccag
    15061 cgaaaattat cgacgatgct gtagctcgca tccgcgagca ggtaggcgac gataaagtca
    15121 tcctcggcct ctctggtggt gtggattcct ccgtaaccgc aatgctgctg caccgtgcta
    15181 tcggtaaaaa cctgacttgc gtattcgtcg acaacggcct gctgcgtctc aacgaagcag
    15241 agcaggttct ggatatgttt ggcgatcact ttggtcttaa cattgttcac gtaccggcag
    15301 aagatcgctt cctgtcagcg ctggctggcg aaaacgatcc ggaagcaaaa cgtaaaatca
    15361 tcgggcgcgt tttcgttgaa gtattcgatg aagaagcgct gaaactggaa gacgtgaagt
    15421 ggctggcgca gggcaccatc taccctgacg ttatcgaatc tgcggcttct gcaaccggta
    15481 aagcacacgt catcaaatct caccacaacg tgggcggcct gccgaaagag atgaagatgg
    15541 gcctggttga accgctgaaa gagctgttca aagacgaagt gcgtaagatt ggtctggagc
    15601 tgggcctgcc gtacgacatg ctgtaccgtc atccgttccc gggaccaggc cttggcgttc
    15661 gtgttctggg tgaagtgaag aaagagtact gtgacctgct gcgccgtgct gacgccatct
    15721 tcattgaaga actgcgtaaa gcggacctgt acgacaaagt cagccaggcg ttcaccgtct
    15781 tcctgccagt acgttccgtt ggcgtaatgg gcgatggtcg taagtatgac tgggttgtct
    15841 ctctgcgtgc tgtcgaaacc atcgacttta tgaccgcaca ctgggcgcat ctgccgtacg
    15901 atttcctcgg tcgcgtttcc aaccgcatta tcaatgaagt gaacggtatt tcccgcgtgg
    15961 tgtatgacat cagcggcaag ccgccagcta ccattgagtg ggaatgattt tgatccctgc
    16021 actatgaatg aacaaaaccc tccgttacta cagagggttt tttatcttca agaatcatag
    16081 gcttgaagtt actaacatcg attaattaaa ccagctgtcc gatttgttct cttctgcttt
    16141 gcccacgctt ttcatcagat cgcgaccgcc ttcagtcata tttctgtttg cgtcagcttc
    16201 agattgcacc acatcggttt gcgcagcttt gtgcttcagt tcctgatcga taaattcgtt
    16261 ttcgcgctta acgcgggctt cttctttcgc cagcgccagt ttttgtttct gaatctctaa
    16321 gctgcgtagc tcatcttcat aactttgatc gcgttttttg tccgcagtgg cttcggcgtc
    16381 cagtttatcc tgacgagctt tcttatttgc cgctgccgtt gccgctcttt tgttcgcggc
    16441 ggcctgggca tttgcgcgac gttgcttctc ttgctggatt tccctgttgc gctccgcgac
    16501 ccattcgtca tgctgccttt gctcttcatt tttaccttgc tgttccgctt ctgcgacagc
    16561 agagagttga tcctgcaatg atgaggctat agccggatag cttaaggagg ctaagatggc
    16621 gcaaagaaaa actttcttca tgactcctcc tgattattag ctcttttcag gacatttggt
    16681 atttggctga atacgcgttt cgttatacgt tgtggtaata acaacggcta aacctgtcgt
    16741 aaactggcac tctttaccct cctgggtgga ggtatacact ttggtgcctt ccttatatgt
    16801 taaagaaaca ccttccacta aggttttatc attcaccata gaacccgctg ccgcgcctac
    16861 agctccgccg ccaactgccc ctgccgtcgt tccggaattg ctgccagaac cgacgttgtg
    16921 gccgataaca ccgccagcga ctgcgccaat aagcgcgccg aaggcttgtg cgttccgttt
    16981 attttgggag ttgtctacgg caacttttgc gggaagaatg gaaataatat taacggtttt
    17041 agtttcttgt ttggtattca gttgatcggt ttgataaaca tcggcggcat gatcgtcagc
    17101 atttgactgg catcctgcca gagtgaatga cgctaacatt gccacaggca gaagacattt
    17161 tttaaatttc atcactattc cttgttatac acattacgaa aaataagaca tttaagatca
    17221 tcaaaagagg aaagtaaatg tattgcgtat aaagggatag attaaattta ttaaaattca
    17281 cgcagggaat aatttataaa aatatatcat tacaacatat atattaattt tatatttatt
    17341 tcgcagccaa acaggataaa catcagtcag gacaaagacg taaaatttaa gatgaaagaa
    17401 tatctgccgc tttgataatc ccaaagagca aaagcatccg acagcgaagt gccgttatgt
    17461 tctgtgaaag tggagaaggt aaccgctaac gttgtaccgg tgattcgacg gagagtgtgg
    17521 attgctgagc ttcttttttg ctctgatggt gctgccaggc accgacggac gagtagataa
    17581 aacggccaaa gaagaagata aagctgatga gcagtacgat acgggtcatg cgactgttaa
    17641 atcggtgtcg ttttcgcata ctagttgcct gactcacaaa aggttccttg aagtatgtcc
    17701 cacgctgtgg acggtactta cattaaggca caacaggaca aaatggtcaa ttcttcgtta
    17761 tgtaaaaaag cgtcagtgga tacatatttt aatgttatgg aagttaattt aattatttac
    17821 aatgatgatg taataatgat gaattaacat gataaacgta ataattcatt aatcagggtt
    17881 attttgtttg atatatatca attggaactt tcatatgaca cttagaatca atgctctcta
    17941 tagtgatgaa taatcatcat tcgaagtcag gtgggatgcc tgtctgaata cacctccttc
    18001 aggatgtggg ggattctgct gagcatctat gaaactgaat gcaacttata taaaaatacg
    18061 tgataaatgg tgggggcttc cgctgttcct gccttcttta atcttgccca ttttcgccca
    18121 cattaatact ttcgcgcata tttcttccgg tgaggttttt ctcttttatc tgcctttggc
    18181 actgatgatc agcatgatga tgtttttcag ctgggcggca ttgccaggga tcaccttagg
    18241 gatttttgtc cgcaaatatg cagagctggg tttttacgaa acgctctcat taacggctaa
    18301 ttttattatc attatcattc tctgttgggg cggttacagg gtctttactc cccggcgtaa
    18361 caacgtttca catggtgata cccgtttaat ttcccagcgt atattctggc agatagtgtt
    18421 tcctgcaacg ctgtttctga tacttttcca gtttgctgca tttgtaggat tactggcgag
    18481 cagagaaaat ctggtcggtg tcatgccttt taacctcggg actttaatca attatcaggc
    18541 cttactggtg ggtaatctga tcggtgtccc gctgtgctac ttcatcattc gggtagtgcg
    18601 aaatccattt tatttacgta gctattattc gcaattaaaa cagcaggttg atgccaaagt
    18661 caccaaaaaa gagttcgcgc tctggctact ggcattaggt gctttattgt tgctgttatg
    18721 catgccgtta aatgaaaaaa gcacaatttt tagcaccaat tataccttgt cattattgct
    18781 gcccctgatg atgtggggag caatgcgcta tggttataag ctgatttcgc tgctctgggc
    18841 ggtcgtgttg atgatcagca tccacagcta tcaaaattac attcccattt atcctggcta
    18901 taccacgcag ctgaccataa cctcctccag ttatctggta ttctctttta ttgtcaatta
    18961 tatggctgta ctggcaactc gtcagcgagc ggtagtcaga cgcattcagc ggcttgcgta
    19021 tgtggacccg gtggttcatc tgccaaatgt tcgcgccctg aatcgcgcgt tgcgtgatgc
    19081 cccctggtct gcgctttgtt atttacgcat ccctggcatg gaaatgctgg ttaagaacta
    19141 tggcatcatg ct
//
LOCUS       DAJTRC010000076        18835 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.076, whole genome shotgun sequence.
ACCESSION   DAJTRC010000076 DAJTRC010000000
VERSION     DAJTRC010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18835)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18835)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18835
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.076"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            1..624
                     /gene="fliF"
                     /locus_tag="OO357_002841"
                     /pseudo
     CDS             1..624
                     /gene="fliF"
                     /locus_tag="OO357_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310704.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
     gene            617..1612
                     /gene="fliG"
                     /locus_tag="OO357_002842"
     CDS             617..1612
                     /gene="fliG"
                     /locus_tag="OO357_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HCR5887726.1"
                     /translation="MSNLTGTDKSVILLMTIGEDRAAEVFKHLSQREVQTLSAAMANV
                     TQISNKQLTDVLAEFEQEAEQFAALNINANDYLRSVLVKALGEERAASLLEDILETRD
                     TASGIETLNFMEPQSAADLIRDEHPQIIATILVHLRRAQAADILALFDERLRHDVMLR
                     IATFGGVQPAALAELTEVLNGLLDGQNLKRSKMGGVRTAAEIINLMKTQQEEAVITAV
                     REFDGELAQKIIDEMFLFENLVDVDDRSIQRLLQEVDSESLLIALKGAEQPLREKFLR
                     NMSQRAADILRDDLAKRGPVRLSQVENEQKAILLIVRRLAETGEMVIGSGEDTYV"
     gene            1605..2291
                     /gene="fliH"
                     /locus_tag="OO357_002843"
     CDS             1605..2291
                     /gene="fliH"
                     /locus_tag="OO357_002843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707825.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar assembly protein FliH"
                     /protein_id="HCR5887727.1"
                     /translation="MSDNLPWKTWTPDDLAPPPAEFVPMVESEETIIEEAEPSLEQQL
                     AQLQMQAHEQGYQAGIAEGRQQGHEQGYQEGLAQGLEQGLAEAKAQQAPIHARMQQLV
                     SEFQTTLDALDSVIASRLMQMALEAARQVIGQTPTVDNSALIKQIQQLLQQEPLFSGK
                     PQLRVHPDDLQRVDDMLGATLSLHGWRLRGDPTLHPGGCKVSADEGDLDASVATRWQE
                     LCRLAAPGVV"
     gene            2291..3664
                     /gene="fliI"
                     /locus_tag="OO357_002844"
     CDS             2291..3664
                     /gene="fliI"
                     /locus_tag="OO357_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellum-specific ATP synthase FliI"
                     /protein_id="HCR5887728.1"
                     /translation="MTTRLTRWLTTLDNFEAKMAQLPAVRRYGRLTRATGLVLEATGL
                     QLPLGATCVIERQNGSETHEVESEVVGFNGQRLFLMPLEEVEGVLPGARVYAKNISAE
                     GLQSGKQLPLGPALLGRVLDGSGKPLDGLPFPDTTETGALITPPFNPLQRTPIEHVLD
                     TGVRPINALLTVGRGQRMGLFAGSGVGKSVLLGMMARYTRADVIVVGLIGERGREVKD
                     FIENILGAEGRARSVVIAAPADVSPLLRMQGAAYATRIAEDFRDRGQHVLLIMDSLTR
                     YAMAQREIALAIGEPPATKGYPPSVFAKLPALVERAGNGISGGGSITAFYTVLTEGDD
                     QQDPIADSARAILDGHIVLSRRLAEAGHYPAIDIEASISRAMTALISEQHYARVRTFK
                     QLLSSFQRNRDLVSVGAYAKGSDPMLDKAIALWPQLEGYLQQGIFERADWEASLQGLE
                     RIFPTVS"
     gene            3686..4027
                     /gene="fliJ"
                     /locus_tag="OO357_002845"
                     /pseudo
     CDS             3686..4027
                     /gene="fliJ"
                     /locus_tag="OO357_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310708.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagella biosynthesis chaperone FliJ"
     gene            4024..5151
                     /gene="fliK"
                     /locus_tag="OO357_002846"
     CDS             4024..5151
                     /gene="fliK"
                     /locus_tag="OO357_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook length control protein FliK"
                     /protein_id="HCR5887729.1"
                     /translation="MIRLAPLITADVDTTTLPGGKASDAAQDFLTLLSEALAGETTTD
                     KAAPQLLVATDKPTTKGEPLVSDILADAQQADLLIPVDETLPVINDEQSTSTPLTTAQ
                     TMTLAAVADKNTTKDEKADDLNEDVTASLSALFAMLPGFDNTPKVTDAPSTVLPAEKP
                     TLFTKLTSAQLTTAQPDDAPGTPAQPLTPLVAEAQSKAEVISTPSPVTADASPLITPH
                     QTQPLPTVAAPVLSAPLGSHEWQQSLSQHISLFTRQGQQSAELRLHPQDLGEVQISLK
                     VDDNQAQIQMISPHQHVRAALEAALPVLRTQLAESGIQLGQSNISGESFSGQQQAASQ
                     QQQSQRTANHEPLAGEDDDTLPVPVSLQGRVTGNSGVDIFA"
     gene            5256..5720
                     /gene="fliL"
                     /locus_tag="OO357_002847"
     CDS             5256..5720
                     /gene="fliL"
                     /locus_tag="OO357_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal body-associated protein FliL"
                     /protein_id="HCR5887730.1"
                     /translation="MTDYAISKKSKRSLWIPILVFITLAACASAGYSYWHSHQVAADD
                     KAQQRVVPSPVFYALDTFTVNLGDADRVLYIGITLRLKDEATRSRLSEYLPEVRSRLL
                     LLFSRQDAAVLATEEGKKNLIAEIKTTLSTPLVAGQPKQDVTDVLYTAFILR"
     gene            5725..6729
                     /gene="fliM"
                     /locus_tag="OO357_002848"
     CDS             5725..6729
                     /gene="fliM"
                     /locus_tag="OO357_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliM"
                     /protein_id="HCR5887731.1"
                     /translation="MGDSILSQAEIDALLNGDSEVKDEPTASISGESDIRPYDPNTQR
                     RVVRERLQALEIINERFARHFRMGLFNLLRRSPDITVGAIRIQPYHEFARNLPVPTNL
                     NLIHLKPLRGTGLVVFSPSLVFIAVDNLFGGDGRFPTKVEGREFTHTEQRVINRMLKL
                     ALEGYSDAWKAINPLEVEYVRSEMQVEFTNITTSPNDIVVNTPFHVEIGNLTGEFNIC
                     LPFSMIEPLRELLVNPPLENSRNEDQNWRDNLVRQVQHSQLELVANFADISLRLSQIL
                     KLKPGDVLPIEKPDRIIAHVDGVPVLTSQYGTLNGQYALRIEHLINPILNSLNEEQPK
                     "
     gene            6726..7139
                     /gene="fliN"
                     /locus_tag="OO357_002849"
     CDS             6726..7139
                     /gene="fliN"
                     /locus_tag="OO357_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HCR5887732.1"
                     /translation="MSDMNNPADDNNGAMDDLWAEALSEQKSTSEKSAADAVFQQFGG
                     GDVSGTLQDIDLIMDIPVKLTVELGRTRMTIKELLRLTQGSVVALDGLAGEPLDILIN
                     GYLIAQGEVVVVADKYGVRITDIITPSERMRRLSR"
     gene            7142..7507
                     /gene="fliO"
                     /locus_tag="OO357_002850"
     CDS             7142..7507
                     /gene="fliO"
                     /locus_tag="OO357_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliO"
                     /protein_id="HCR5887733.1"
                     /translation="MNNHATVQSSAPVSAEPLLQVSGALIAIIALILAAAWLVKRLGF
                     APKRTGVNGLKISASASLGARERVVVVDVEDARLVLGVTAGQINLLHKLPPSAPTEEI
                     PQTDFQSVMKNLLKRGGRS"
     gene            7507..8244
                     /gene="fliP"
                     /locus_tag="OO357_002851"
     CDS             7507..8244
                     /gene="fliP"
                     /locus_tag="OO357_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310714.1"
                     /note="The bacterial flagellar biogenesis protein FliP
                     forms a type III secretion system (T3SS)-type pore
                     required for flagellar assembly; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system pore protein
                     FliP"
                     /protein_id="HCR5887734.1"
                     /translation="MRRLFSVAPVLLWLITPLAFAQLPGITSQPLPGGGQSWSLPVQT
                     LVFITSLTFIPAILLMMTSFTRIIIVFGLLRNALGTPSAPPNQVLLGLALFLTFFIMS
                     PVIDKIYVDAYQPFSEEKISMQEALEKGAQPLREFMLRQTREADLGLFARLANTGPLQ
                     GPEAVPMRILLPAYVTSELKTAFQIGFTIFIPFLIIDLVIASVLMALGMMMVPPATIA
                     LPFKLMLFVLVDGWQLLVGSLAQSFYS"
     gene            8254..8523
                     /gene="fliQ"
                     /locus_tag="OO357_002852"
     CDS             8254..8523
                     /gene="fliQ"
                     /locus_tag="OO357_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013365797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HCR5887735.1"
                     /translation="MTPESVMMMGTEAMKVALALAAPLLLVALVTGLIISILQAATQI
                     NEMTLSFIPKIIAVFIAIIIAGPWMLNLLLDYVRTLFTNLPYIIG"
     gene            8532..9317
                     /gene="fliR"
                     /locus_tag="OO357_002853"
     CDS             8532..9317
                     /gene="fliR"
                     /locus_tag="OO357_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707835.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FliR"
                     /protein_id="HCR5887736.1"
                     /translation="MMQETSDQWLSWLSLYFWPLLRVLALISTAPILSERSVPKRVKL
                     GLAMMITFAIAPSLPANDVPVFSFFALWLAVQQILIGIALGFTMQFAFAAVRTAGEII
                     GLQMGLSFATFVDPGSHLNMPVLARIMDMLALLLFLTFNGHLWLISLLVDTFHTLPIG
                     SEPLNSNAFLAPTKAGSLIFLNGLMLALPLITLLLTLNLALGLLNRMAPQLSIFVIGF
                     PLTLTVGISLMAALMPLIAPFCEHLFSEIFNLLADIISELPLI"
     gene            9602..10225
                     /gene="rcsA"
                     /locus_tag="OO357_002854"
     CDS             9602..10225
                     /gene="rcsA"
                     /locus_tag="OO357_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator RcsA"
                     /protein_id="HCR5887737.1"
                     /translation="MSTIIMDLCSYTRLGLTGYLLSRGVKKREINDIETVDDLAIACD
                     SQRPSVVFINEDCFIHDASNSQRIKHIINQHPNTLFIVFMAIANVHFDEYLLVRKNLL
                     ISSKSIKPESLDDILGDILKKETTITSFLNMPTLSLSRTESSMLRMWMAGQGTIQISD
                     QMNIKAKTVSSHKGNIKRKIKTHNKQVIYHVVRLTDNVTNGIFVNMR"
     gene            complement(10269..10457)
                     /gene="dsrB"
                     /locus_tag="OO357_002855"
     CDS             complement(10269..10457)
                     /gene="dsrB"
                     /locus_tag="OO357_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707837.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein DsrB"
                     /protein_id="HCR5887738.1"
                     /translation="MKVNDRVTVKTDGGPRRPGVVLAVEEFSEGTMYLVALEDYPLGI
                     WFFNEAGHQDGIFVEKAE"
     gene            10620..10847
                     /gene="yodD"
                     /locus_tag="OO357_002856"
     CDS             10620..10847
                     /gene="yodD"
                     /locus_tag="OO357_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxide/acid resistance protein YodD"
                     /protein_id="HCR5887739.1"
                     /translation="MKTAKVYSDTAKREVSVDVDALLAAINEISESEVHRSQNDSEHV
                     SVDGREYHTWRELADAFELDIHDFSVSEVNR"
     gene            11145..11960
                     /locus_tag="OO357_002857"
                     /pseudo
     CDS             11145..11960
                     /locus_tag="OO357_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-3-phosphoglycerate phosphatase-related
                     protein"
     gene            complement(11957..13651)
                     /gene="yedQ"
                     /locus_tag="OO357_002858"
                     /pseudo
     CDS             complement(11957..13651)
                     /gene="yedQ"
                     /locus_tag="OO357_002858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416465.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis regulator YedQ"
     gene            complement(13822..14004)
                     /locus_tag="OO357_002859"
     CDS             complement(13822..14004)
                     /locus_tag="OO357_002859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2158 domain-containing protein"
                     /protein_id="HCR5887740.1"
                     /translation="MSFMVSEEVTVKEGGPRMIVTGYSSGMVECRWYDGYGVKREAFH
                     ETDLVPGEGSRSAEEV"
     gene            complement(14083..15000)
                     /locus_tag="OO357_002860"
     CDS             complement(14083..15000)
                     /locus_tag="OO357_002860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF808 domain-containing protein"
                     /protein_id="HCR5887741.1"
                     /translation="MLLAGSSLLTLLDDIATLLDDISVMGKLAAKKTAGVLGDDLSLN
                     AQQVSGVRANRELPVVWGVAKGSLINKVILVPLALIISAFIPWAITPLLMIGGAFLCF
                     EGVEKVLHMLEARKHKEDPAQSQQRLEKLAAQDPLKFEKDKIKGAIRTDFILSAEIVA
                     ITLGIVAEAPLLNQVLVLSGIALVVTVGVYGLVGVIVKIDDLGYWLAEKSSALMQALG
                     KGLLIIAPWLMKALSIVGTLAMFLVGGGIVVHGIAPLHHAIEHIAGQQSAVVAMILPT
                     VLNLILGFIIGGIVVLGVKAVAKMRGQAH"
     gene            15173..16093
                     /gene="yedA"
                     /locus_tag="OO357_002861"
     CDS             15173..16093
                     /gene="yedA"
                     /locus_tag="OO357_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HCR5887742.1"
                     /translation="MRFRQLLPLFGALFALYIIWGSTYFVIRIGVGSWPPLMMAGVRF
                     QAAGILLLAFLLLRGHKLPPLRPLLNAALIGLLLLAVGNGMVTVAEHQNVPSGIAAVV
                     IATVPLFTLCFSRLFGIKTRKLEWVGIAIGLAGIIMLNSGGNLSGNPWGAILILIGSI
                     SWAFGSVYGSRITLPVGMMAGAIEMLAAGVVLMIASMIAGEKLTALPSLSGFLAVGYL
                     ALFGSIIAINAYMYLIRNVSPALATSYAYVNPVVAVLLGTGLGGETLSKIEWLALGVI
                     VFAVVLVTLGKYLFPAKPVVAPVIQDASSE"
     gene            complement(16082..16552)
                     /gene="vsr"
                     /locus_tag="OO357_002862"
     CDS             complement(16082..16552)
                     /gene="vsr"
                     /locus_tag="OO357_002862"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VSPR family DNA mismatch endonuclease"
                     /protein_id="HCR5887743.1"
                     /translation="MVDVHDKATRSKNMRAIATRDTAIEKRLASLLTGQGLAFRVQDA
                     SLPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDIS
                     RLQELGWRVLIVWECALRRREKLTDAALTERLEEWICGEGASAQIDTQGIHLLA"
     gene            complement(16533..17951)
                     /gene="dcm"
                     /locus_tag="OO357_002863"
     CDS             complement(16533..17951)
                     /gene="dcm"
                     /locus_tag="OO357_002863"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-cytosine methyltransferase"
                     /protein_id="HCR5887744.1"
                     /translation="MQENISVTDSYSTGNAAQAMLEKLLQIYDVKMLVAQLNGVGENH
                     WSAAILKRALANDSAWHRLSEKEFAHLQTLLPKPPEHHPHYAFRFIDLFAGIGGIRRG
                     FESIGGQCVFTSEWNKHAVRTYKANHYCDPATHHFNEDIRDITLSHQEGVSDEAAAEH
                     IRQHIPEHDVLLAGFPCQPFSLAGVSKKNSLGRAHGFACDTQGTLFFDVVRIIDARRP
                     AMFVLENVKNLKSHDKGKTFRIIMQTLDELGYDVADAEDNGPDDPKIIDGKHFLPQHR
                     ERIVLVGFRRDLNLKADFTLRDISECFPAQRVTLAQLLDPMVEAKYILTPVLWKYLYR
                     YAKKHQARGNGFGYGMVYPNNPQSVTRTLSARYYKDGAEILIDRGWDMATGEKDFDDP
                     LNQQHRPRRLTPRECARLMGFEAPGEAKFRIPVSDTQAYRQFGNSVVVPVFAAVAKLL
                     EPKIKQAVALRQQEAQHGRRSR"
     gene            complement(18018..18558)
                     /locus_tag="OO357_002864"
                     /pseudo
     CDS             complement(18018..18558)
                     /locus_tag="OO357_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310727.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
     gene            complement(18598..>18835)
                     /locus_tag="OO357_002865"
     CDS             complement(18598..>18835)
                     /locus_tag="OO357_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887745.1"
                     /translation="WAFYTWCGYFVWAMARYIWVMSRIPDAPVSGFESDLGSTAGKWL
                     GALVGFLFMALVGALLGGIAWYTRPRPARCRRYE"
BASE COUNT         4518 a         5013 c         4872 g         4432 t
ORIGIN      
        1 gtagcacaca gcggaatgaa accagtaact acgaagtcga tcgcaccatt cgtcatacca
       61 aaatgaacgt gggcgatgtg caacgtctgt cagtcgcggt agtggtgaat tacaaaacct
      121 tgccagacgg taaaccattg cctctcagca acgaacagat gaagcaaatt gaagctctga
      181 cccgcgaggc gatgggcttt tctgaaaaac gcggtgattc gctcaatgtt gttaactcgc
      241 cgttcaatag cagtgacgaa agcggcggag cactgccgtt ttggcaacag caagtgttta
      301 tcgatcagtt gctggctgcc ggtcgctggt tgctggttgc tggtactgct ggtggcgtgg
      361 ttgctgtggc ggaaagcggt acgcccgcag ctaacacgtc gcgccgaggc ggtgaagact
      421 gtgcagcaac aggcgcaggc ccgcgaggaa gtggaagatg cggtggaagt ccgtctgagc
      481 aaagacgaac aactccaaca acggcgcgct aaccaacgac tgggggcaga agtcatgagc
      541 cagcgtatcc gtgaaatgtc tgataacgat ccgcgcgtgg tggcgctggt cattcgccag
      601 tggataaata acgatcatga gtaacctgac aggcaccgat aaaagcgtca tcctgctgat
      661 gaccattggc gaagaccggg cggcagaggt gttcaagcac ctctcccagc gcgaagtgca
      721 aaccctgagc gctgcaatgg cgaacgtcac gcagatctcc aacaagcagc taaccgatgt
      781 gctggcggag tttgagcaag aagctgaaca gtttgctgca ctgaatatca acgccaacga
      841 ttatctgcgt tcggtattgg tcaaagctct gggtgaagaa cgtgccgcca gcctgctgga
      901 agatattctc gaaacccgcg ataccgccag cggtattgaa acgctcaact ttatggagcc
      961 gcagagcgcc gccgatctga ttcgcgatga gcatccgcaa attatcgcca ccattctggt
     1021 gcatctgagg cgcgcccagg ccgccgatat tctggcgttg ttcgatgaac gtctgcgcca
     1081 cgacgtgatg ttgcgtatcg ccacctttgg cggcgtgcag ccagccgcgc tggccgaact
     1141 gaccgaagta ctgaatggct tgctcgacgg tcagaatctc aagcgcagca aaatgggcgg
     1201 cgtgagaacg gcagccgaaa ttatcaacct gatgaaaacg cagcaggaag aagccgttat
     1261 taccgccgtg cgtgaattcg acggcgagct ggcgcagaaa atcatcgacg agatgttcct
     1321 gttcgagaat ctggtggatg tcgatgatcg cagcattcag cgtctgttgc aggaagtgga
     1381 ttccgaatcg ctgctgatcg cgctgaaagg ggccgagcag ccactgcgcg agaaattcct
     1441 gcgcaatatg tcgcagcgtg ccgccgatat tctgcgcgac gatctcgcca agcgtggtcc
     1501 ggtgcgtctg tcgcaggtcg aaaacgaaca gaaagcgatt ctgctgattg tgcgccgtct
     1561 tgccgaaact ggcgagatgg tgattggcag cggcgaggat acctatgtct gataatctgc
     1621 cgtggaaaac ctggacgccg gacgatctcg cgccaccacc ggcagagttt gtgccaatgg
     1681 tcgagtcgga agaaaccatt attgaagagg ccgaacccag ccttgagcag caactggcgc
     1741 aactgcaaat gcaggcccac gagcagggtt atcaggcggg gattgccgaa ggtcgccagc
     1801 aaggtcacga acagggctat caggaaggac tggcccaggg gctggaacaa ggtctggcag
     1861 aggcgaaggc tcaacaagcg ccaattcatg cccggatgca gcaactggtc agcgaatttc
     1921 aaactaccct tgatgcgctt gatagtgtga tagcttcgcg cctgatgcag atggcgctgg
     1981 aggcagcacg tcaggtgatc ggtcagacac caacggtgga taactcggca ctgatcaaac
     2041 agatccaaca gttgttgcag caagaaccgt tattcagcgg gaaaccacag ctgcgcgtac
     2101 acccggatga tctgcaacgt gtggatgata tgctcggcgc aactttaagt ttgcatggct
     2161 ggcgcttgcg gggcgatccc accctccacc ctggcggatg taaagtctcc gccgatgaag
     2221 gcgatctcga cgccagtgtc gccactcgct ggcaagaact ctgccgtctg gcagcaccag
     2281 gagtggtgta atgaccacgc gcctgactcg ctggctaacc acgctggata actttgaagc
     2341 caaaatggcg cagttgcctg cggtacgtcg ctacgggcga ttaacccgcg ctaccgggct
     2401 ggtgctggaa gccaccggat tacaattgcc gctcggcgca acctgtgtca ttgagcgcca
     2461 gaacggcagc gaaacgcacg aagtagaaag cgaagtcgtt ggctttaacg gtcaacggct
     2521 gtttttaatg ccgctggagg aagtggaagg tgtcctgccc ggcgcgcgtg tatatgccaa
     2581 aaacatttcg gcagaagggc tgcaaagcgg caagcagttg ccgctcggtc cggcgttatt
     2641 aggtcgtgtt ctggacggta gcggtaaacc gctcgatggc ctgcccttcc ccgatacgac
     2701 ggaaaccggt gcgctgatta ccccgccatt taacccgttg caacgtacac cgattgaaca
     2761 tgtgctggac accggcgtgc gcccaatcaa tgccctgctt accgtcggac gcgggcagcg
     2821 tatggggctg tttgccgggt cgggcgttgg taaaagtgtg ctgctgggga tgatggcacg
     2881 ctacacccgc gccgatgtca ttgtcgtggg tttgattggt gaacgtgggc gcgaagtaaa
     2941 agattttatt gagaatatcc tcggtgccga aggacgtgca cgctcagtgg tgattgccgc
     3001 tccggcggat gtttctccgc tcctgcgaat gcaaggtgcc gcctatgcca cgcgcattgc
     3061 cgaagatttt cgcgatcgtg gtcagcatgt gttgctgatt atggactccc tcacccgcta
     3121 cgcgatggcc cagcgtgaaa ttgcgctggc gattggcgaa ccacctgcca ctaaaggtta
     3181 tccaccgtcg gtgtttgcca aattaccggc actggtcgag cgtgccggta atggcattag
     3241 cggcggcggc tcgattaccg cgttttatac cgtgctcacc gaaggcgatg accagcagga
     3301 ccccattgct gactccgcgc gggcaatcct cgacggtcac attgtgctgt ctcgccgact
     3361 ggcggaagcc gggcactatc cggctatcga tattgaagcg tcgatcagtc gcgcaatgac
     3421 ggcgttgatc agtgagcaac attacgcgcg agtgcgcacc ttcaaacagc tgttgtcgag
     3481 ttttcagcgt aaccgcgatc tggttagcgt cggcgcgtat gccaaaggca gcgatccgat
     3541 gctcgataaa gccatcgccc tgtggccgca gctggagggc tatttgcaac aaggcatttt
     3601 tgaacgcgcg gactgggaag cgtctctcca ggggctggag cgtattttcc cgacagtgtc
     3661 ataacccagg agataacggc agatggcgga agaacagctc aaaatgttga tcgattatca
     3721 gaatgaatat cgcaataacc tcaatagcga tatgagtgcc gggatgacca gcaaccgctg
     3781 gatcaactat cagcagttta tccagacgct ggaaaaagcc attactcagc atcgccagca
     3841 acttaatcag tggacgcaga aagttgacat tgccctgaac agctggcgag aaaagaaaca
     3901 acgtttgcag gcctggcaga cactgcagga acggcaatcc acggcggcac tgcttgcaga
     3961 aaaccgcctc gatcagaaaa agatggatga gttcgcccag cgcgccgcca tgaggaaacc
     4021 tgaatgattc gcttagcgcc cttgattacc gccgacgttg acaccaccac attgcctggc
     4081 ggcaaagcca gcgatgctgc acaagatttt ctcacgttgt tgagcgaagc attagcaggc
     4141 gagacaacaa ctgacaaagc ggccccccag ttgctggtag caacagataa gcccacgaca
     4201 aaaggcgagc cgctggtcag cgatattctt gccgatgcgc aacaagcgga tttactgatc
     4261 cctgtggatg aaacacttcc tgtcatcaac gacgaacaat ccacatcaac accgttaacc
     4321 accgctcaga cgatgacgtt ggctgcggtg gctgacaaaa atacgacaaa agacgaaaaa
     4381 gcggatgatc ttaatgaaga cgtcaccgca agtctgagcg ccctttttgc gatgttgccg
     4441 ggttttgaca atacgcccaa agtgactgat gcaccgtcaa ccgtgttacc ggcagagaaa
     4501 ccaacgctct tcacaaaact gacttctgcg caactcacta cagcacagcc tgatgacgcc
     4561 cccggcacac cagctcagcc attaacaccg ctggtagcag aagcccagag taaagcggaa
     4621 gtcatcagca cgccttcgcc ggtgaccgct gacgccagcc cgctaatcac tccacaccag
     4681 acacagccac tgcccaccgt cgccgcgcct gttttgagtg caccgctggg ttctcacgaa
     4741 tggcaacaat cattgagcca gcatatttcg ctgttcaccc gccaggggca acaaagtgca
     4801 gagttgcgtc tgcacccaca ggatttaggt gaagtgcaaa tttccctcaa agtggatgat
     4861 aaccaggcgc agatccagat gatttcaccg catcagcacg tacgcgccgc cctggaagca
     4921 gcgctgccgg tactgcgtac gcagctggcc gaaagtggca ttcagttagg gcaaagcaac
     4981 attagtggcg aaagctttag tggtcagcag caggccgctt cccagcaaca gcaaagccaa
     5041 cgcacagcaa accatgaacc tctggcgggg gaagacgacg atacgcttcc ggttcccgtc
     5101 tctttacaag ggcgtgtaac aggtaacagc ggcgttgata ttttcgccta acgtcagagg
     5161 tagcaccgta atccgcgtct tttccccgct ttgttgcgct caagacgcag gataattagc
     5221 cgataagcag tagcgacaca ggaagaccgc aacacatgac tgattacgcg ataagcaaga
     5281 aaagcaagcg atcgctttgg atcccgattc tggtattcat taccctcgcg gcctgtgcca
     5341 gcgcaggtta cagctactgg cattcgcatc aggttgccgc tgacgacaaa gcgcagcaac
     5401 gcgtcgtgcc ctccccggtc ttctatgcgc tggatacctt tacggtcaat ttgggcgatg
     5461 cggatcgcgt gctttatatc ggcataaccc tgcgactgaa agatgaagca acccgctcgc
     5521 ggctgagtga gtatttgccg gaagtccgta gtcgcttgct gttactgttt tcacgtcagg
     5581 atgctgccgt actggcgaca gaagaaggca agaaaaacct gattgccgag attaaaacca
     5641 cactttccac cccgcttgtt gccgggcaac cgaaacagga tgtcaccgac gtgctgtata
     5701 ccgcttttat tctgcgataa cgacatgggc gatagtattc tttctcaagc tgaaattgat
     5761 gcgctgttga atggtgacag cgaagtcaaa gacgaaccga cagccagtat tagcggcgaa
     5821 agtgacattc gtccgtacga tccgaatacc caacgacggg ttgtgcgcga acgtttgcag
     5881 gcgctggaaa tcattaatga gcgctttgcc cgccattttc gtatggggct gttcaacctg
     5941 ctgcgccgta gcccggacat taccgtcggg gccatccgca ttcagccgta ccatgaattt
     6001 gcccgcaacc tgccggtgcc gaccaacctg aacctaatcc acctgaaacc gctacgcggc
     6061 acagggctgg tagtgttctc gccgagtctg gtgtttatcg ccgtggataa cctgtttggc
     6121 ggcgatggac gcttcccgac caaagtggaa ggtcgcgagt ttacccatac tgaacagcgc
     6181 gtcatcaacc gtatgttgaa gctggcgctg gaaggctata gcgacgcctg gaaagcgatt
     6241 aatccgctgg aagttgagta cgtgcgttcg gaaatgcagg tggaatttac caatatcacc
     6301 acctcgccga acgacattgt ggttaacacg ccgttccatg tggagattgg caacctgacc
     6361 ggcgaattta atatctgcct gccattcagc atgatcgagc cgctacggga attgttggtt
     6421 aacccgccgc tggaaaactc gcgtaatgaa gatcagaact ggcgcgataa cctggtgcgc
     6481 caggtgcagc attcacagct ggaactggtg gccaactttg ccgatatctc gctacgcctg
     6541 tcgcagattt taaaactgaa acccggcgac gtcctgccga tagaaaaacc cgatcgcatc
     6601 atcgcccatg ttgacggcgt cccggttctg accagtcagt acggtactct caacggtcag
     6661 tatgcgttac ggatagaaca tttgattaac ccgattttaa attctctgaa cgaggaacag
     6721 cccaaatgag tgacatgaat aatccggccg atgacaacaa cggcgcaatg gacgatctgt
     6781 gggctgaagc gttgagcgag caaaaatcaa ccagcgaaaa aagcgctgcc gacgcggtgt
     6841 tccagcaatt tggcggtggt gatgtcagcg gaacgttgca ggatatcgac ctgattatgg
     6901 atattccggt caagctgacc gtcgagctgg gccgtacgcg gatgaccatc aaagagctgt
     6961 tgcgtctgac gcaagggtca gtcgtggcgc tggacggtct ggcgggcgaa ccgctggata
     7021 ttctgatcaa cggttattta atcgcccagg gcgaagtggt ggttgttgcc gataaatatg
     7081 gcgtgcggat caccgatatc attaccccgt ccgagcggat gcgccgcctg agccgttagt
     7141 gatgaataac catgctactg tgcaatcttc cgcgccggtt tctgctgagc cactgctgca
     7201 ggtgagcggc gcactcatcg ccattattgc cctgatcctc gctgctgcct ggctggtaaa
     7261 acggctggga tttgccccta aacgcactgg cgttaacggt ctgaaaatta gcgccagtgc
     7321 ttcgctgggc gcgcgtgaac gggtcgtggt ggtcgatgtg gaagatgcac ggctggtgct
     7381 cggcgttacc gcagggcaaa tcaatctgct gcataaactt cccccttcag caccaacgga
     7441 agagataccg cagaccgatt ttcagtcggt catgaaaaat ttgcttaagc gtggcgggag
     7501 atcctgatgc gtcgtttgtt ttctgtcgca ccggtccttc tctggctgat tacgcccctc
     7561 gccttcgcgc aactgccggg tatcaccagc cagccgctac ctggcggtgg acaaagctgg
     7621 tcgctcccgg tgcagacgct ggtgttcatc acctcgttga cgttcattcc agcgatttta
     7681 ctgatgatga ccagtttcac ccgcatcatt attgtttttg gtttattgcg taacgcgctg
     7741 ggaacgccct ccgcgccacc taaccaggta ttgctggggc tggcactgtt tttgaccttt
     7801 tttattatgt cgccagtgat cgacaaaatt tatgtcgatg cgtaccagcc attcagcgaa
     7861 gagaaaatct caatgcagga ggcgctggaa aaaggggcgc agccgctgcg tgagtttatg
     7921 ctgcgtcaga cccgcgaggc agatttaggg ttgtttgcca gactggcgaa taccggcccg
     7981 ttgcaggggc ctgaagctgt gccgatgcgc attttgctcc cggcctacgt gaccagcgag
     8041 ttgaaaaccg catttcagat aggcttcacg attttcatcc cttttttgat tatcgacctg
     8101 gtgattgcca gcgtgttgat ggcattgggg atgatgatgg ttccccccgc caccattgct
     8161 ctgcccttta aactgatgct gtttgtactg gtggatggct ggcaattgct ggtcggttcg
     8221 ctggcgcaga gcttttacag ctagagaggc aaaatgacac ctgaatcggt catgatgatg
     8281 ggaactgaag cgatgaaagt cgcgctggca ctggcagccc cgctgttgct ggtagcgctg
     8341 gtcacgggcc ttatcatcag tattttgcag gcagccacgc agattaacga aatgacgctg
     8401 tcgtttattc cgaaaatcat cgccgtattt atcgccatca ttattgccgg accgtggatg
     8461 ctcaatctgt tgctggatta cgtccgcacc ttgttcacta acctgccgta tatcatcggg
     8521 tagcccgtac aatgatgcag gagacaagcg atcaatggct ttcctggtta agcctgtact
     8581 tctggccgtt actgcgcgtg ctggcgctca tttctaccgc gccaattctg agcgaacgca
     8641 gcgtgccgaa acgggtaaaa ctgggtctgg caatgatgat cacgttcgcc attgccccat
     8701 cattacctgc caacgatgtt cctgtttttt cgttctttgc tctgtggctg gccgtacagc
     8761 agatcctgat cggcattgcg cttggtttta ccatgcaatt tgcctttgcc gctgtgcgaa
     8821 ccgctggcga aattatcggt ctgcaaatgg ggctgtcatt tgcgacattt gtcgatcctg
     8881 gcagccatct caatatgccc gttttagcgc gcatcatgga tatgctggca ttactgctgt
     8941 tcctgacatt taacggtcat ttatggttga tttcactgct ggtcgatacc tttcacaccc
     9001 tgccgattgg tagcgaaccg ttgaacagca atgcgtttct ggcacccacc aaagcaggga
     9061 gtttgatttt ccttaacggg ctgatgctgg cgttaccgct cattactctg ttgctgaccc
     9121 tgaatctggc attaggttta ctgaatcgta tggccccgca attatccatt tttgttattg
     9181 gatttccatt aactctgact gtcggcatct ctttaatggc ggcattaatg ccgttaattg
     9241 cgcctttttg cgaacattta ttcagtgaaa tttttaattt gctggctgat attattagtg
     9301 aattaccatt aatataattc cgtaacgttt atcatgctac cctaaggatt atccgaaaaa
     9361 taatatctgc gaacatcttc caggatactc ctgtagcgaa atatttgttt taagctcact
     9421 cacatatcgc aacatttact ttaagacaat tccaggcaaa ttatacaaca ctttacggga
     9481 tagtaagtcc gcctgaaaaa ttgcgagagt ggcgcattag gtgacccatg ttgttccgtt
     9541 tagtcatgat gaaatattca ggtaagggga attatcgtta cgcattgagt gagggtatgc
     9601 catgtcaacg attattatgg atttatgtag ttacacccga ctaggtttaa ccgggtatct
     9661 gttgagtaga ggggttaaaa aaagagaaat caacgacatt gaaaccgttg atgaccttgc
     9721 catagcttgt gattcacagc gtccttcagt ggtgtttatt aatgaggact gtttcatcca
     9781 cgatgcttct aacagtcagc gcatcaagca catcattaat caacatccca atacgttatt
     9841 tatcgttttt atggcaattg ccaatgttca ttttgatgaa tatctattgg tcagaaaaaa
     9901 tttattgatc agttctaaat cgattaaacc ggaatctctc gacgatatcc ttggcgatat
     9961 tctgaaaaaa gagacaacga taacctcgtt tttaaatatg ccgacgttat cattgagccg
    10021 aaccgaatcg agtatgttgc gaatgtggat ggcaggtcag ggaaccattc aaatctccga
    10081 ccaaatgaat atcaaagcca agaccgtttc atcgcataaa ggtaatatta aacgtaagat
    10141 caaaacgcat aataaacagg ttatctacca tgtcgtccga ctgacggata atgtgactaa
    10201 tggtattttt gtcaacatgc gctaacacat tctgactggt ggtttcccac cagtcaggct
    10261 gaataagatt actctgcttt ctcaacaaag ataccgtcct gatgccctgc ttcattaaag
    10321 aaccagatgc cgagcgggta gtcttccagc gcaaccaggt acattgtgcc ttcactaaac
    10381 tcctcaactg ccagtaccac tccaggacga cgcggaccgc catccgtttt gactgttacc
    10441 cgatcattca ccttcatcgt tttcctcctg tggctttgtg ccagtgtaga acaatttcgt
    10501 ttttctggca gcgccaagcg cgcgcgagtg ctgattttct cgacggtcta tacttaagag
    10561 atgccaggcg gacttaacga ctggcggcaa caacagagta acggttgcga ggaaagatga
    10621 tgaaaaccgc aaaagtgtac agcgataccg caaaacgtga ggtcagcgtc gatgtcgatg
    10681 ccctgctggc ggcgatcaat gaaattagcg aaagcgaagt tcatcgcagc cagaacgatt
    10741 ctgaacacgt tagcgtcgat ggacgtgaat atcatacatg gcgtgaattg gcggatgcct
    10801 tcgaactgga tattcatgac ttcagcgtct ctgaagtgaa tcgttgaatg cacaataaaa
    10861 aaatcccgac cctgagggtg tcgggatgaa acttgcttaa gcaagaagca cttaaaaaat
    10921 tcgttacacc aggaaatctg atgtgttcat caccttatct gcaatttttt tcgctgacaa
    10981 gaaaatattc gcgctatgaa tgattaacgc tacttatgaa tagaaatgtg acgcgcgtca
    11041 ccccttggtg gctctggtgt caacgggatc gctcccgaaa aaatcatctt tgattatttg
    11101 tgctgttttg agaatcatcc tggtaagggg agtactgcgc gaccatgttt tcaattcaac
    11161 aaccactact ggtttttagc gatcttgatg gcaccctgct ggacagtcat agttatgact
    11221 ggcaaccggc agccccctgg ctcagccgtt tacacgaagc caatattccc gtcattctct
    11281 gtagcagtaa aactttagcg gaaatgctgt acttgcaaaa aatgttgggg ctacaaggct
    11341 taccgctgat tgcagagaat ggcgcagtga tccagcttgc tgaacaatgg caggatatag
    11401 acggttttcc acgcatcatc tcaggtatta gccatggcga aatctgccag gttttaaata
    11461 cgctacgcga gaaagaacat tttaaattca cgacttttga tgatgtcgac gatgcaacca
    11521 ttgccgaatg gacgggatta agccgtagct aggcggcgct gacgcagctg catgaggcgt
    11581 cggtaacgct aatctggcgc gacagtgacg agcgtatggc acaatttatc gctcgtctga
    11641 acgaactggg cttgcagttt atgcagggtg cgcgcttctg gcacgtactg gatgcctctg
    11701 ccggaaaaga tcaggctgcc aactggatta tcgcgaccta tcaacaattg tcaggcagac
    11761 gcccaaccac acttggcctg ggcgatgggc caaacgatgc gcctttactg gaggtaatgg
    11821 attacgcggt gattgtgaaa gggctaaacc gtgaaggggt acatctgcat gatgaggatc
    11881 cggcccgcgt ctggcgaacg cagcgtgaag gaccggaagg ctggcgtgaa gggctggacc
    11941 attttttctc cgcccgttaa gcgttatcgc tcgcgaatac ccgattgcgc ccggcctgtt
    12001 ttgcgaggta aagccgacgg tcggccaatg actggagttg ttcaaaatca taatcaccgg
    12061 tttcctcgct gctacttacc cccagcgagg cactgatgcg tatcgttgta ctcttggcga
    12121 tcagcatctc tttttcattt aacttcaggc gaatacgttc tgcgacctcc gcagcctgcg
    12181 tcagattcgc gcctggcaga atcacacaaa actcctcacc accgacccgc ccggcaacgt
    12241 cctgcgcacg caaggaactg ctaattaatc cggcagcatg agaaagaaca cggtcgcccg
    12301 cctgatgacc aaagcggtca ttaatcgctt taaaatggtc aagatcgacc tgaatgacag
    12361 aaaaaggatg ttggtgcgtc tgacacaatt tagcgagcgg acgggctttt tcgaacagtg
    12421 cgccacggtt atataaacgc gttaaggtgt cgtgccacgc ctgccactgc aacgagcttt
    12481 gcagaacata catgttgctg accatccggc gaatcacata ccaggagagg agtaacatgg
    12541 tggtaaagag cgcccacagc agggttaatg caatgctgat actgccgaaa tcgccgcgca
    12601 cgccttcgct tagcgtatgg acgcgcgcca gcacaccgtc gaaatggtcc agacgttccc
    12661 agctaacata gcgactgtcc atacgaatgc cgccccgcgt gtcatgttcc attgcctgcg
    12721 ccagcaaggc cagttcacga ggatcaaaaa tattccctgt tggatggtca ggattggaag
    12781 aggtcaaaaa tctcagctta ctgtcataga gctgatactc accatcgagg tttttatcga
    12841 tggcgtttct taaaaattgc tgcatagtac gcacgggaat actcatcccc agcacgccat
    12901 accagtagtt attactgtct accggaacac tgacggtaac ctgcggttca gtattgctgg
    12961 cgtgttccgg ttgcgaagtg aaccagcgta ccgcgcggtg acgattttct cgttgcgaat
    13021 ggccgataaa ccagggttgg gtgacatagc cgtaataacg cgttggtaca ttgcgcgtaa
    13081 acaaggtcgg ctgcgtcgaa acgtaaaatc cagcacgcga gacatacatc gcctgttcaa
    13141 ccatcgacga ggagttgtgc gccagtcgca gcaagtaacc aacttccagt gcagcggtaa
    13201 tttcattgtc gaggctttca ttttcgcgag acaggaaatt cccctcgctg actaatgcat
    13261 ccgatacgcc attgactggc agggtgcgcc gtcggttgag ttcgatttgc caggcgtgct
    13321 cgtcgcgatg ctgttcgaac tcggttacgg cattacgcag agaggtgaaa tccagtggcg
    13381 ccacgagagc ttcgcgcatg ccattacgca aaaagatcag tttgtcgacg ttatactgca
    13441 aatgcttatc gagcgcgttg gctacgtttt ccagatgatt acgctagctg gagatatagg
    13501 catcttccag caccaccact tcacgccagg tgagcaaggt ggaaaaaagc aataccacga
    13561 taaagcagag attaacgaca tgaccgggcc ccaggcggcg tgcgagtttt ttcaaccagc
    13621 tctggttttc catttttgtc tcgtgctgca ccctgacgac tcccaacctg cttttttatg
    13681 attctggcat aacccggcaa tagcgtaccg caaaacagcg ataattttat aatggtggca
    13741 tgataagacg cgactatcat tcattgcagg cattgggaca ccaggccgga tgtgatccgg
    13801 cctggtgtta atgataccct ttcaaacttc ttccgcagaa cgactcccct cccccggaac
    13861 aagatcggtt tcatgaaaag cttcccgctt gaccccgtaa ccgtcatacc aacgacactc
    13921 aaccataccg ctggagtatc cagtgacaat catccgcggg ccgccctctt taaccgtaac
    13981 ttcctcacta accataaagc tcatactcgc ctcctttttt cgagtgaaac ttgttcacct
    14041 tagttgaaga tggcgaattt tgcataatag ccagtgcgag tattaatgtg cctgaccgcg
    14101 cattttcgct actgctttca ctcccagcac cacgatgccg ccgatgataa atccaagaat
    14161 cagatttaaa acagtcggta atatcatcgc caccactgca ctttgctgcc cggcgatatg
    14221 ttcaatagcg tgatgcagcg gcgcaatacc atgtaccaca atcccgccgc cgacgaggaa
    14281 catcgccagc gtgccgacaa tcgataatgc tttcatcagc caaggcgcga taatcaataa
    14341 ccctttacct aatgcctgca tcagcgcgct agatttttcc gccagccaat accctaggtc
    14401 atcaatctta acgataaccc ctaccagacc gtagacgccc acggtcacta ccagcgcgat
    14461 gcctgaaagc accagcacct gattaagcaa cggcgcctct gccacaatcc ccagcgtgat
    14521 ggcgacgatt tccgcagaca aaataaaatc ggtacgaatc gcccctttaa ttttgtcctt
    14581 ttcaaacttc agcggatcct gcgccgccag cttctccaga cgctgctggc tctgcgctgg
    14641 atcttcttta tgtttacgcg cctccagcat atgcagcact ttctctaccc cttcaaagca
    14701 gagaaacgcg ccaccaatca tcaacagtgg cgtaatcgcc cacgggataa acgcactgat
    14761 gatcagcgcc agcggcacca gaatcacttt attgatcagc gatcctttcg ccacgcccca
    14821 gaccacggga agttcccggt tggcccgcac gcctgaaact tgttgcgcat tgagcgataa
    14881 atcatcccct aatacaccgg cggttttctt cgccgccagt ttgcccatca cagagatatc
    14941 gtccagcagt gtggcgatat catcgagcaa cgtcagtaaa ctactcccgg ccaaaagcat
    15001 tccctctcat ttttattgtt gaagggtgta agtatgaagc aaaaacggca gctctgatac
    15061 ttgcttcaca ggtcaacatt tatttaacaa tcaagtgcaa ttatatctct cgccagcaga
    15121 atgattttca cgtttactat gaaccacctt tttatttccg ccgtgaggga ttatgcgttt
    15181 ccgccagttg ttaccgcttt ttggcgcgct gtttgcgttg tatatcattt ggggctcaac
    15241 ctattttgtc attcggattg gcgtgggaag ctggcctccg ttaatgatgg cgggcgttcg
    15301 attccaggca gccggtattt tattgctggc atttttgcta ctgcgcggac acaaacttcc
    15361 cccgctacgt ccgctgctca atgccgcgct gattggcctg ttattgctgg ctgtcggtaa
    15421 tggcatggtg acggttgccg aacatcaaaa tgttccttcc ggcatcgccg ccgtagtgat
    15481 tgcaaccgtg cccctcttta ccctgtgctt cagtcgcctg tttggcatta aaacgcgcaa
    15541 actggaatgg gtgggtattg ccattgggct tgccggaatc atcatgctca atagcggtgg
    15601 aaatttaagc ggcaatccgt ggggcgcgat tctgatttta atcggctcga ttagctgggc
    15661 gtttggctca gtttatggct cgcgcattac cttacctgta gggatgatgg cgggtgcgat
    15721 tgagatgctg gcggcaggcg tggtgttaat gatcgcatcg atgattgcgg gtgaaaaact
    15781 gacggcgctc ccttccctgt caggcttcct tgcggtcggc tatctggcgc tgtttggttc
    15841 gattatcgcc attaacgctt atatgtattt aatccgtaat gtcagtccgg ctctcgccac
    15901 cagctacgcc tacgttaacc cggtggtcgc ggtcttgctg ggtacgggac tgggtggcga
    15961 aacgctgtcg aagattgaat ggctggcgct cggcgtaatt gttttcgcgg tggtactggt
    16021 cacgttggga aaatatctct tcccggccaa acccgttgtc gcgccagtta ttcaggatgc
    16081 atcaagcgag taaatgaatc ccctgcgtgt cgatctgcgc gctggcacct tcgccgcaga
    16141 tccactcttc cagacgctcg gtaagcgccg catccgtcag cttctcgcgc ctacgtaacg
    16201 cacactccca gacaatcagt acgcgccagc cgagctcctg caagcgactg atatcgcggc
    16261 gatcgcgttc aacattttta cctatcttct ccagccagaa ctcagttcga gtcgcaggca
    16321 ctttaaacag atagcagtga tgatgatgcc agaagcagcc gtgggtaaat atcacgcagc
    16381 gatattcatc aacgacaaaa tccgggcgtc cgggcagact ggcgtcctga acgcgaaacg
    16441 caaggccctg cccggttaac aaactggcga ggcgcttctc aatcgccgta tcacgcgtgg
    16501 caatcgcgcg catatttttg ctgcgggtgg ccttatcgtg aacgtcgacc atgttgtgcc
    16561 tcttgctgac gcaacgccac cgcctgtttg atttttggct caagcagttt tgccaccgcg
    16621 gcaaagaccg gcacgaccac cgagttgccg aactggcgat aggcctgagt gtccgaaacc
    16681 ggaatgcgga atttcgcttc tcccggcgct tcaaaaccca ttaagcgcgc gcattcacga
    16741 ggcgttaacc gacgtggacg atgttgctga ttcagcggat cgtcaaagtc tttctcaccc
    16801 gtggccatat cccaaccgcg atcgattaaa atttccgcgc catctttgta ataacgagca
    16861 gacagcgtac gggtgacgct ttgcggattg ttcggataaa ccattccata accgaagccg
    16921 ttaccgcgcg cctgatgttt tttcgcatat cgatagaggt acttccacag caccggcgtc
    16981 aggatatatt tcgcctcaac catcgggtcc agcagctgcg ccagcgtcac tcgctgtgca
    17041 gggaaacatt cgctgatatc acgcagagta aaatcggctt tcagattcag atcgcgacga
    17101 aaacccacca gcacgatgcg ttcacggtgc tgcggcagga aatgtttacc atcgatgatt
    17161 ttcggatcgt ccggcccgtt atcttctgca tcggcaacgt catagcccag ttcgtccagc
    17221 gtctgcatga tgatgcggaa cgtttttccc ttatcgtgac tcttcaggtt tttgacgttt
    17281 tcgagcacaa acatcgccgg acgacgcgcg tcgataatgc gcaccacatc aaagaacagc
    17341 gtgccctggg tatcgcaggc aaaaccgtgc gcccgcccga gcgagttctt tttcgatacg
    17401 ccagccagcg aaaatggctg acaagggaaa ccggccagta aaacatcgtg ttcaggaatg
    17461 tgttgacgaa tatgttccgc cgccgcctca tcactcacgc cttcttgatg actgagggtg
    17521 atgtcgcgga tatcttcatt aaaatgatgc gtcgccgggt cgcaataatg gttggcttta
    17581 taagtgcgta ccgcatgttt gttccattcg ctggtaaaca cgcactgtcc accaatcgat
    17641 tcaaaaccac gacggatgcc gccaattccg gcaaatagat cgataaagcg aaacgcataa
    17701 tgcggatgat gttccggtgg tttgggtaac aacgtttgca gatgggcaaa ctctttctca
    17761 cttaaacggt gccatgccga gtcattcgcc agcgcacgtt ttaaaattgc cgcgctccag
    17821 tgattctcac ccacaccatt aagctgcgcc accagcattt taacatcata aatttgcagc
    17881 agtttctcca gcattgcctg tgcggcattc ccggtgctgt atgaatcggt tactgatata
    17941 ttttcctgca tagatttaac cggccatctg agatggccga caggttaaca taattactgc
    18001 cgtttagggc cacgcgctca gccagcgggt gaaaacgcat ctatcacctt gtgatcgata
    18061 ccttcattct cccccgccag ttcggcaccg agcttcgcca tcaaacagcg ccaagctcaa
    18121 tgcccctgaa acggcaaata cgcgcgccag gccaatcgcc cccagggctt ccagccgatc
    18181 cgcatcctgg acgattttag cttccgttgt taagggggcg atttgcgcgc tgaaactgtg
    18241 agcggcaatg gcatgacaaa cggcctcaat tttctccgcc ggaaattgca cgaactcttc
    18301 acggagcagg cgacgcgtct cttctgccgc caggattgaa gaacgctgcc gttgcggatg
    18361 atttttcgcc aggctcacga tatcgtgaaa ataacaggcg gttaaaatca ccagcatatc
    18421 gacatcgtca tctgccgcca gtttctgtgc cgtagcccag acacggcgaa aatgacacag
    18481 gtcatgcgcc gcgtcctgat gttgatgatg gtttttcaac cagttctcga actatgcctg
    18541 ccagtgttgt aactccatac gcgctccgtt gccaaagagt ctcaacctta gcagttttta
    18601 ttcatagcgt ctgcaacgtg cgggacgagg tcgggtgtac catgcaatgc cgcctaacaa
    18661 ggcaccaacc agggccataa acagaaatcc gaccaatgct cctaaccact tccccgcggt
    18721 agatcctaaa tcactttcaa atcccgacac gggggcatcg gggatccggc tcatcaccca
    18781 gatgtatcgc gccattgccc agacgaaata gccacaccat gtatagaaag cccac
//
LOCUS       DAJTRC010000077        18803 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.077, whole genome shotgun sequence.
ACCESSION   DAJTRC010000077 DAJTRC010000000
VERSION     DAJTRC010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18803)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18803)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18803
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.077"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(139..3654)
                     /locus_tag="OO357_002866"
     CDS             complement(139..3654)
                     /locus_tag="OO357_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5887746.1"
                     /translation="MDENALGFASYWRNSLADAESGKGSFKRKDAQNFTHWHGIAAGR
                     LDEAIVSKFFEGEKDDVETVDVILRPKVYFRLLQHGKDRSAGAPDIVTPIVTPALLSR
                     EGFLYPTPATSIPRDLLEPLPKGAFSIGEIGQYDKYKTTHTTFSINFDDSVDKTAETD
                     EEREARYAALQQEWRQYLYDSERLLKSVAGDWIEKPEQYELAEHGYIVKTAQSGGASS
                     HILSLYDHLLVCNKDVPLFNRFASREVHAAESLLAPGAKFSDRLGHSGDKFPLAKAQR
                     DALSHFLDARHGDILAVNGPPGTGKTTLVLSIIATQWARAALEKSEPPVIIATSTNNQ
                     AVTNIIEAFGKDFSQGSGAMAGRWLPELKSFGAYFPSSSRKAEAAKKYQTEDFFNQVE
                     SKEYVEDALLFYLEKAKAAFPGKECSSPEKVIELLHGQLAAKSEQLIRLNATWQTLSQ
                     IRAARELIANDIEQYLDNLNKLLSGQEQKVTLLKSAKTEWKKYRAGESLIYSLFSWLP
                     AVRNKRQYQIQLFLEDKLGALIAGNQWSDPETIERNIDGLLNSAEREQTTYRQQIDSA
                     HEIVLKEQQAVQEWQRLAFDLGYEGDEELSFSQADELADTQIRFPAFLLTTHYWEGRW
                     LMDMASIDDLQDEKKKKGAKGVTARWQRRMKLTPCVVMTCYMLPGNMQISEHKGQRKF
                     EKSYLYDFADLLIVDEAGQVLPEVAAASFALAKKALVIGDTEQIPPIWSIAPAIDVGN
                     MLAEKILSGSTQEEITEKYTAIADLGKSAASGSVMKIAQFASRYQYDPELARGMYLYE
                     HRRCYDNIIGYCNTLCYHGKLLPKRGREESNLMPAMGYLHIDGKGELASSGSRYNLLE
                     AETIAVWLAENQQNIEAHYGKSLHEVVGIVTPFSAQVSTIKQVLGKQDISTGTNEKSL
                     TVGTVHSLQGAERAIVIFSPVYSKHEDGGFIDSDNSMLNVAVSRAKDSFLVFGDMDLF
                     EVQPASSPRGLLAKYLFESEKNALSFDYKERKDLKTAGTKIYTLHGVEQHDNFLNQTF
                     ENTSKHITIISPWLTWQRLEQTGFLDSMIAACSRGINVTIVTDRSYNTEHNDFEKRKE
                     KQQNFKAALEKLNALGIATKLVNRVHSKIVIGDDGLLCVGSFNWFSATREARYERYDT
                     SMVYCGDNLKGEIEAIYNSLERRQV"
     gene            complement(4107..5369)
                     /locus_tag="OO357_002867"
     CDS             complement(4107..5369)
                     /locus_tag="OO357_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5887747.1"
                     /translation="MALTDAKIRAAKPTDKAYKLTDGAGMFLLVHPNGSRYWRLRYRI
                     LGKEKTLALGVYPEVSLSEARTKRDEARKLISEGIDPCEQKRVKKVVPDLQLSFEHIA
                     RRWHASNKQWAQSHSDKVLKSLETHVFPFIGNRDITTLNTPDLLIPVRAAEAKQIYEI
                     ASRLQQRISAVMRYAVQSGIIRYNPALDMAGALTTVKRQHRPALELSRLPELLSRIDG
                     YKGQPVTRLAVMLNLLVFIRSSELRYARWSEIDIDNSMWTIPAEREPLPGVKFSHRGS
                     KMRTPHLVPLSKQVVAILAELQTWAGENGLIFTGAHDPRKPISENTVNKALRVMGYDT
                     TQEVCGHGFRAMACSALIESGLWSRDAVERQMSHQERNGVRAAYIHKAEHLEERRLML
                     QWWADFLDANREKGISPFEYAKINNPLK"
     gene            complement(5567..5642)
                     /locus_tag="OO357_002868"
     tRNA            complement(5567..5642)
                     /locus_tag="OO357_002868"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5607..5609),aa:Phe,seq:gaa)
     gene            complement(5748..6455)
                     /locus_tag="OO357_002869"
     CDS             complement(5748..6455)
                     /locus_tag="OO357_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HCR5887748.1"
                     /translation="MVIGPFINASAVLLGGVLGALLSQRLPERIRVSMTSIFGLASLG
                     IGILLVVKCANLPAMVLATLLGALIGEICLLEKGVNTAVAKAQNLFRHSRKKPAHESF
                     IQNYVAIIVLFCASGTGIFGAMNEGMTGDPSILIAKSFLDFFTAMIFACSLGIAVSVI
                     SIPLLIIQLTLAWAAALILPLITPSMMADFSAVGGLLLLATGLRICGIKMFPVVNMLP
                     ALLLAMPLSAAWTAWFA"
     gene            6853..8988
                     /gene="speC"
                     /locus_tag="OO357_002870"
     CDS             6853..8988
                     /gene="speC"
                     /locus_tag="OO357_002870"
                     /EC_number="4.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311868.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HCR5887749.1"
                     /translation="MKSMNIAASSELVSRLSSHRRVVALGDTDFTDVAAVVITAADSR
                     SGILALLKRTGFHLPVFLYSEHAVELPAGVTAVINGNEQQWLELESAACQYEENLLPP
                     FYDTLTQYVEMGNSTFACPGHQHGAFFKKHPAGRHFYDFFGENVFRADMCNADVKLGD
                     LLIHEGSAKDAQKFAAKVFHADKTYFVLNGTSAANKVVTNALLTRGDLVLFDRNNHKS
                     NHHGALIQAGTTPVYLEASRNPFGFIGGIDAHCFNEEYLRQQIRDVAPEKADLPRPFR
                     LTIIQLGTYDGTVYNARQVIDTIGHLCDYILFDSAWVGYEQFISMMADSSPLLLELNE
                     NDPGIFVTQLVHKQQAGFSQTSQIHKKDNHIRGQARFCPHKRLNNAFMLHASTSPFYP
                     LFAALDVNAKIHEGESGRRLWAECVELGIESRKAILARCKLFRPFIPPVVDGKLWQDY
                     PTSVLASDRRFFSFEPGAKWHGFEGYAADQYFVDPCKLLLTTPGINAETGEYSDFGVP
                     ATILAHYLRENGIVPEKCDLNSILFLLTPAESHEKLAQLVAMLAQFEQHIEDDSSLAE
                     VLPSVYNKYPVRYRDYTLRQLCQEMHDLYVSFDVKDLQKAMFRQQSFPSVVMNPQDAH
                     SAYIRGEVELVRIRDAEGRIAAEGALPYPPGVLCVVPGEVWGGAVQRYFLALEEGVNL
                     LPGFSPELQGVYSETDADGMKRLYGYVLK"
     gene            complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO357_002871"
     CDS             complement(9037..10293)
                     /gene="nupG"
                     /locus_tag="OO357_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311867.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside permease NupG"
                     /protein_id="HCR5887750.1"
                     /translation="MNLKLQLKILSFLQFCLWGSWLTTLGSYMFVTLKFDGASIGAVY
                     SSLGIAAVFMPALLGIVADKWLSAKWVYAICHTIGAITLFMAAQVTTPEAMFLVILIN
                     SFAYMPTLGLINTISYYRLQNAGMDIVTDFPPIRIWGTIGFIMAMWVVSLSGFELSHM
                     QLYIGAALSAILVLFTLTLPHIPVAKQQANQSWTTLLGLDAFALFKNKRMAIFFIFSM
                     LLGAELQITNMFGNTFLHSFDKDPMFASSFIVQHASIIMSISQISETLFILTIPFFLS
                     RYGIKNVMMISIVAWILRFALFAYGDPTPFGTVLLVLSMIVYGCAFDFFNISGSVFVE
                     KEVSPAIRASAQGMFLMMTNGFGCILGGIVSGKVVEMYTQNGITDWQTVWLIFAGYSV
                     VLAFAFMAMFKYKHVRVPTGTQTVSH"
     gene            complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO357_002872"
     CDS             complement(10495..11574)
                     /gene="mltC"
                     /locus_tag="OO357_002872"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane-bound lytic murein transglycosylase
                     MltC"
                     /protein_id="HCR5887751.1"
                     /translation="MKKYLALALIAPLLISCSTTKKGDTYNEAWVKDTNGFDILMGQF
                     AHNIENIWGFKEVVIAGPKDYVKYTDQYQTRSHINFDDGTITIETIAGTEPAAHLRRA
                     IIKTLLMGDDPSSVDLYSDVDDITISKEPFLYGQVVDNTGQPIRWEGRASNFADYLLK
                     NRLKSRSNGLRIIYSVTINMVPNHLDKRAHKYLGMVRQASRKYGVDESLILAIMQTES
                     SFNPYAVSRSDALGLMQVVQNTAGKDVFRSQGKSGTPSRSFLFDPASNIDTGTAYLAM
                     LNNVYLGGIDNPTSRRYAVITAYNGGAGSVLRVFSNDKIQAANIINTMTPGDVYQTLT
                     TRHPSAESRRYLYKVNTAQKSYRRR"
     gene            complement(11639..11914)
                     /locus_tag="OO357_002873"
     CDS             complement(11639..11914)
                     /locus_tag="OO357_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidative damage protection protein"
                     /protein_id="HCR5887752.1"
                     /translation="MSRTIFCTFLQREAEGQDFQLYPGELGKRIYNEISKEAWAQWQH
                     KQTMLINEKKLNMMNAEHRKLLEQEMVNFLFEGKEVHIEGYTPEDKK"
     gene            complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO357_002874"
     CDS             complement(11942..12994)
                     /gene="mutY"
                     /locus_tag="OO357_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HCR5887753.1"
                     /translation="MQASQFSAQVLDWYDKYGRKTLPWQIDKTPYKVWLSEVMLQQTQ
                     VATVIPYFERFMAHFPTVTDLANAPLDEVLHLWTGLGYYARARNLHKAAQQVATLHGG
                     KFPETFEEVAALPGVGRSTAGAILSLSLGKHFPILDGNVKRVLARCYAVSGWPGKKEV
                     ENKLWSLSEQVTPAVGVERFNQAMMDLGAMICTRSKPKCSLCPLQNGCIAATNNSWSL
                     YPGKKPKQTLPERTGYFLLLQHEDEVLLAQRPPSGLWGGLYCFPQFADEESLRHWLAQ
                     RQIAADNLTQLTAFRHTFSHFHLDIVPMWLPVSSFTGCMDEGNALWYNLAQPPSVGLA
                     APVERLLQQLRTGAPV"
     gene            13155..13874
                     /gene="trmB"
                     /locus_tag="OO357_002875"
     CDS             13155..13874
                     /gene="trmB"
                     /locus_tag="OO357_002875"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HCR5887754.1"
                     /translation="MKNDVISPEFDENGRPLRRIRSFVRRQGRLTKVQEHALENYWPV
                     MGVEFSEDMLDFPALFGREAPVTLEIGFGMGASLVAMAKDRPEQDFLGIEVHSPGVGA
                     CLASAHEEGLSNLRVMCHDAVEVLHKMIPDNSLRMVQLFFPDPWHKARHNKRRIVQVP
                     FAELVKSKLQLGGVFHMATDWEPYAEHMLEVMSSIDGYKNLSESNDYVPRPASRPVTK
                     FEQRGHRLGHGVWDLMFERVK"
     gene            13874..14200
                     /locus_tag="OO357_002876"
     CDS             13874..14200
                     /locus_tag="OO357_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggL family protein"
                     /protein_id="HCR5887755.1"
                     /translation="MAKNRSRRLRKKMHIDEFQELGFSVAWRFPEGTSEEQIDKIVDD
                     FINEVIEPNKLAFDGSGYLAWEGLICMQEIGKCTEEHQAIVRKWLEECKLDEVRTSEL
                     FDVWWD"
     gene            14384..15103
                     /locus_tag="OO357_002877"
     CDS             14384..15103
                     /locus_tag="OO357_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2884 domain-containing protein"
                     /protein_id="HCR5887756.1"
                     /translation="MMRKMLLAAALSVTAMTAHADYQCSVTPRDDVIVSPQTVQVKGE
                     NGNLVITPDGNVMYNGKQYSLNAAQREQAKDYQAELRSTLPWIDEGAKSRVEKARIAL
                     DKIIVQEMGDSSKMRSRLTKLDAQLKEQMNRIIETRSDGLTFHYKAIDQVRAEGQQLV
                     NQAMGGILQDSINEMGAKAVLKSGGNPLQNVLGSLGGLQSSIQTEWKKQEKDFQQFGK
                     DVCSRVVTLEDSRKALVGNLK"
     gene            15279..16325
                     /gene="ansB"
                     /locus_tag="OO357_002878"
     CDS             15279..16325
                     /gene="ansB"
                     /locus_tag="OO357_002878"
                     /EC_number="3.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-asparaginase 2"
                     /protein_id="HCR5887757.1"
                     /translation="MEFFKKTALAALVMGFSGAALALPNITILATGGTIAGGGDSATK
                     SNYTAGKVGVENLVNAVPQLKDIANVKGEQVVNIGSQDMNDNVWLTLAKKINADCDKT
                     DGFVITHGTDTMEETAYFLDLTVKCDKPVVMVGAMRPSTSMSADGPFNLYNAVVTAAD
                     KASANRGVLVVMNDTVLDGRDVTKTNTTDVATFKSVNYGPLGYIHNGKIDYQRTPARK
                     HTSDTPFDVSKLNELPKVGIVYNYANASDLPAKALVDAGYDGIVSAGVGNGNLYKSVF
                     DTLATAAKNGTAVVRSSRVPTGATTQDAEVDDAKYGFVASGTLNPQKARVLLQLALTQ
                     TKDPQQIQQIFNQY"
     gene            16442..17404
                     /locus_tag="OO357_002879"
     CDS             16442..17404
                     /locus_tag="OO357_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1202 family protein"
                     /protein_id="HCR5887758.1"
                     /translation="MKKQWIVGTALLMLMTGNAWADGEPPTENILKDQFKKQYHGILK
                     LDAISLKNLDAKGNQATWSAEGDVSSSDDLYTWVGQLADYELLEQTWTKDKPVKFSAM
                     LTSKGTPASGWSVNFYSFQAAASDRGRVVDDIKTNNKYLIVNSEDFNYRFSQLESALN
                     TQKNSIPALEKEVKALDKQMVAAQKAADAYWGKDANGKQMTREEAFKKIHQQRDEFNK
                     QNDSEAFAVKYDKEVYQPAIAACHKQSEECYEVPIQQKRDFDINEQRRQTFLQSQKLS
                     RKLQDDWVTLEKGQYPLTMKVSEINSKKVAILMKIDDINQANER"
     gene            complement(17505..>18803)
                     /locus_tag="OO357_002880"
     CDS             complement(17505..>18803)
                     /locus_tag="OO357_002880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708726.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HCR5887759.1"
                     /translation="DSYIAITLFGCFFVLVFIGVPISFSIGIATVASMLLMFPWDIAA
                     ITVSQRLANGLDNFALLAIPFFIFAGTLMNSGGIAIRLINLAQVMVGRVPGSLGHVNV
                     LANMMFGSISGSAVAAAAAVGGTLNPIQTKEGYDPAFSTAVNVSSCITGLLIPPSNVL
                     IVFSLTAGGVSVASLFMAGYLPGILMGLAVMIVCGIIAKRRGYPLSERATFAQACKAF
                     LDALPSLLLVFIVMGGILGGIFTATEASAIAVVYTFILSVLIYREVKWRHLPKLILES
                     VVTTSIVLLLIGFSVGMSWAMTNADIPYMISDALMGISDNPLIILLLINIVLLIVGIF
                     MDMTPAVLIFTPIFLPIAQELGMDPVHFGIMMVANLCIGLLTPPVGSALFVGCSISGV
                     KIQHLIKPLLLFYAALLVALMMITYIPQISLFIPQLLGLM"
BASE COUNT         4899 a         4584 c         4643 g         4677 t
ORIGIN      
        1 gagcctctga ttcttccgga cagaagataa tcatgtcggt atgaatgtta taattaagga
       61 ctgtgcaata atagccattc cattcagcca tcttacgctc gaaaacgatc ggtaagctgg
      121 ctactcacca ttcctgctct aaacctgacg cctctcaaga ctattataaa tagcctcaat
      181 ctcacccttc aggttatcac cgcaataaac cattgatgta tcgtatcgtt catatcgcgc
      241 ttcccgtgtc gcactaaacc agttgaacga tcccacacac agcaaaccat catcaccaat
      301 aacaattttg ctatgaacac ggtttaccag ctttgtagca atacccagcg cattcagttt
      361 ctccagcgcc gctttaaagt tctgctgctt ctcttttcgc ttctcaaaat cattatgttc
      421 agtgttgtag cttctgtcag tgactatcgt gacgttaatt ccacgtgaac acgccgcaat
      481 catggaatca agaaaaccgg tttgctccag cctttgccag gtcagccatg gagaaattat
      541 cgtgatgtgt ttactggtat tttcaaatgt ctgattcagg aaattatcat gttgctccac
      601 accatgaagt gtgtagattt tggtcccggc ggtttttaaa tccttacgct ctttataatc
      661 aaaagagagc gcattcttct ctgactcaaa gaggtatttt gccagtaatc cccgtggcga
      721 tgaggctggc tggacctcaa acaggtccat atcgccgaag accagaaaac tgtccttcgc
      781 acgggagact gcaacattca gcatgctgtt atcgctatca ataaacccgc cgtcttcatg
      841 ttttgaatag actggcgaga atatcacaat cgctctttcc gctccctgaa gagagtgcac
      901 ggtgcccact gtgagcgact tttcattcgt gcctgtactg atatcttgtt tgcccagcac
      961 ctgtttgata gtggatacct gagcgctaaa aggcgtcaca ataccgacaa cttcatgaag
     1021 cgatttaccg taatgcgctt caatattttg ctggttctct gccaaccaga ccgctatcgt
     1081 ttcagcctca agcaaattat atcgacttcc actacttgcc agctctcctt taccatcaat
     1141 atggagatac cccattgcgg gcattaaatt gctctcttca cgccctcttt taggcaacaa
     1201 cttaccgtga tagcagagcg tattacagta tccaataata ttgtcgtagc accggcggtg
     1261 ttcatatagg tacataccac gagccagttc gggatcatat tgatagcgcg aagcaaactg
     1321 cgctattttc ataacgctgc cagatgcggc acttttacca aggtctgcga ttgccgtata
     1381 tttctcggta atctcttctt gcgtactgcc agacagaatt ttttccgcca gcatgttacc
     1441 gacatcaatc gcaggagcaa tactccatat tggcgggatc tgctccgtat cgccaatcac
     1501 taatgccttc ttagctaatg caaacgaggc agcagccact tcaggaagca cctgcccggc
     1561 ttcatcgaca atgagtaaat cggcaaaatc atacaaataa cttttctcga atttacgttg
     1621 tcctttgtgc tcacttatct gcatattacc gggcagcata tagcatgtca tcaccacaca
     1681 tggcgtgagt ttcattcgac gttgccaacg ggcggttacc cctttagcac cttttttctt
     1741 cttctcatcc tgcagatcat caatgctggc catatccatc agccaacgac cttcccagta
     1801 gtgagtcgtc agtaaaaatg cagggaagcg aatctgcgta tcagccagtt catcggcctg
     1861 tgagaagctc agttcctcgt cgccctcata ccctaaatca aatgccagcc tctgccactc
     1921 ctgaaccgcc tgctgttctt taagaacgat ttcatgggcg gagtcaatct gctgccggta
     1981 tgttgtttgc tcgcgctcag cggaattgag cagcccatca atattacgtt cgatagtttc
     2041 aggatcagac cactgatttc ctgcaatcag cgcgcctaat ttatcttcga gaaacagctg
     2101 tatttggtac tgtcgcttat tgcgaaccgc cgggagccag gaaaataatg aatagatcag
     2161 tgattcaccg gcgcgatatt ttttccattc cgttttagca ctcttcagta gagtgacttt
     2221 ttgttcttgt ccggaaagta atttatttaa attatcgaga tattgctcaa tatcattagc
     2281 aataagctca cgcgcagccc gaatctggct taacgtttgc catgttgcgt tcagtcttat
     2341 cagttgctca gattttgctg ccaactgacc atgcaagagt tcaatgacct tttcagggga
     2401 tgaacactct tttccaggaa aggctgcctt agccttttcc agataaaaca gcagtgcatc
     2461 ctctacatac tcttttgact caacctggtt gaagaaatct tcagtttgat attttttggc
     2521 tgcctcagct ttacgactgc ttgagggaaa ataagcaccg aagcttttca gctctggcaa
     2581 ccatcgcccg gccatcgcac ctgaaccttg cgaaaagtct ttcccgaatg cctcaataat
     2641 gttcgttaca gcctggttat tcgttgaagt cgcgataata accggaggct cagatttttc
     2701 gagagccgct ctggcccact gcgtggcgat gatagaaagc accagcgtgg tttttccggt
     2761 tcccggaggg ccattaacag caaggatatc gccatgtctt gcatccagaa aatggcttaa
     2821 ggcatcgcgc tgagcctttg ccagcggaaa cttatctccg gagtgtccaa gcctgtcgct
     2881 gaattttgct cctggggcca gcaaagactc tgcagcatga acctctcgcg aggcgaagcg
     2941 attgaagagc ggcacatcct tattgcaaac aagcaggtga tcataaagag aaaggatatg
     3001 ggaactggca ccgccagatt gagccgtttt aacaatataa ccgtgctcag cgagttcata
     3061 ttgctcaggt ttttcaatcc agtcgccggc aacgctcttc agtagcctct ctgagtcata
     3121 cagatattga cgccactcct gctgcaaggc ggcatatcgt gcttcccgtt cttcatccgt
     3181 ttcggcagtc ttatcaacgc tgtcatcaaa gttgatagag aacgtggtat gggtcgtctt
     3241 gtatttgtca tactgcccaa tctcaccaat cgaaaatgct ccttttggca aaggttcaag
     3301 caggtctctg ggaatggagg tcgctggcgt cggatataaa aaaccctcac ggcttagcaa
     3361 ggctggcgtc actatcgggg taacaatatc aggcgcgcct gcggaacggt ccttaccatg
     3421 ctgcagtaac cggaaataaa cttttgggcg caagatgaca tcgaccgttt cgacatcgtc
     3481 tttttctccc tcaaaaaatt tactgacaat cgcttcgtca agacgtcccg ccgctatccc
     3541 atgccagtga gtgaaatttt gggcgtcttt ccgtttaaaa ctgccctttc ctgactcagc
     3601 atccgcaagc gagttgcgcc agtatgaggc aaaccctaaa gcattttcat ccattaccag
     3661 tcctgtgact caatgaaatc cattgattta tcgaatataa aatcaccgta tggacagcta
     3721 ttgtccatat tgaaaaagtt ggatgcaatg ttttgtttat aattcgatat caaatgaact
     3781 ggttgtacaa ggttcagaac acaagctcat taaacagtac gtcaggacta ttgatttcat
     3841 aacagtgaat acatttttta ccgtttataa taactacctg tgttggacgg ggataccaga
     3901 gcagccgaaa acaccggggg ttattacctg ctctcatgga aaccatatat tcctggtgct
     3961 aatcacagtc ataaatagcc tgaagaacgt gacgaaggga ctttaatgga agccggagta
     4021 acttgtcaga aacatcaatc ttcgtcatca tttacaaaga aaaataatta gttaaagcag
     4081 tccgggctga ttgcccggac tgcttactat tttaatggat tgttaatctt tgcatattca
     4141 aacgggctga tacccttctc ccggttcgca tctagaaaat cggcccacca ttgcaacatc
     4201 agtcgccgtt cttccagatg ttctgcttta tggatatacg cagcacgtac accattacgc
     4261 tcctgatggc tcatctgacg ttccacagca tcgcgggacc acaaacctga ttcaatcaat
     4321 gcactgcacg ccatcgcccg gaatccatgg ccacagactt cctgggttgt gtcatacccc
     4381 atcaccctca gggctttatt tacagtattt tcactgattg gtttacgcgg gtcatgtgca
     4441 cccgtaaaga tcagaccatt ttcaccagcc catgtctgaa gttctgccag tatggctaca
     4501 acctgtttgc tgagtggcac aagatgtggt gttcgcatct tggatccccg gtgtgagaat
     4561 tttacgcctg gcagaggttc gcgttcggct ggaatagtcc acatggaatt gtcaatatca
     4621 atttccgacc agcgggcata tctgagttca ctggaacgaa taaaaaccag taaattcagc
     4681 ataactgcca gtcgggtgac aggctggcct ttataaccgt caatacgcga cagaagttca
     4741 ggcaggcgtg aaagctccag agcagggcga tgctggcgtt ttaccgtggt caatgcacca
     4801 gccatatcca gagccgggtt atatctgatg atgccagact gtacggcgta acgcattaca
     4861 gcagatattc tttgctgcag acgactggcg atttcataaa tttgttttgc ttctgcagca
     4921 cgaacaggga taagcaggtc cggggtattg agtgttgtga tatcccggtt gccgataaag
     4981 gggaaaacgt gtgtctcgag gcttttgagt actttatcgc tgtgtgattg tgcccattgt
     5041 ttattactgg catgccagcg tcgtgcaata tgttcaaaag agagctgtaa atcagggaca
     5101 acttttttaa ctcttttctg ttcgcaaggg tcaatcccct ctgaaatcag ttttcgggcc
     5161 tcatcccgtt ttgtacgagc ttcggagaga gaaacttctg gatacacacc aagtgccaga
     5221 gtcttctcct tacccagaat acgataacgg agacgccagt aacgggaacc attaggatga
     5281 accagcagga acatgccggc cccgtcagtg agtttataag ccttgtcagt gggctttgca
     5341 gcccggattt ttgcgtcagt cagtgccata gttcagcctt ctgaatgcgt taaagagggt
     5401 acaaattctg tcgaactggg gtatacccgc aattataccc acacaatggg gttgatgtgg
     5461 ggggaatctg gttgagttcg gtagaattga tttggaggaa gaacacttaa atcatggtgt
     5521 tctgaaacga aaaacggacc tccgtggagg tccgttgata tgaatttggt gcccggactc
     5581 ggaatcgaac caaggacacg gggattttca atcccctgct ctaccgactg agctatccgg
     5641 gcaacggggc gcattaaacc tgattcgcct cgtctcgtca atcaaatttc ttcaatttac
     5701 tgcagactgc acaatctatc atcactttgc cgctattgca cgcattgtca ggcaaaccag
     5761 gcggtccagg cggcggaaag cggcattgcc agaaggagtg ctggaagcat gttgaccacc
     5821 gggaacattt taatgccaca gatgcgtaat ccggttgcca gcagcaataa accgcctacg
     5881 gcgctgaagt ctgccatcat cgacggtgtg atcagcggta atatcagcgc ggcagcccac
     5941 gccagcgtta actggatgat cagtaatggg atactaatta ccgacaccgc aatacccagc
     6001 gagcaggcga agatcatcgc cgtaaagaaa tcaagaaatg acttggcgat taaaatactc
     6061 ggatcgccgg tcatcccttc gttcatcgcc ccgaagatcc cggtgccgct ggcgcaaaac
     6121 aggacaataa tcgcgacata attctgaata aaagattcat gcgctggctt cttacgtgag
     6181 tgacgaaaca gattttgtgc tttggcgact gctgtattga cacctttctc cagcaaacaa
     6241 atttcgccaa ttagagcgcc aagtagcgta gctaaaacca tcgctggaag gttggcgcat
     6301 ttcacaacca gtaaaatacc aatccccagc gatgccagac caaaaataga tgtcatggag
     6361 acgcggatac gttccggtaa gcgttggctg agcagtgcgc cgagaacgcc acccagtaag
     6421 actgcactag cgttgataaa agggccgatg accacaagag ttcctgttag ctgtattgtt
     6481 tgattttctt attatgaact ttttgccttg ttgatggctt gtctggtcgc aaagtcctgg
     6541 cttgcacgcc ttagcgaaag gtgccatgat tgcgcgaatt ttctcctctc tgtacggagt
     6601 ttgcccgatg cacgccatct ccttacattc tctcgcttat cgccgtttcg cgcgaaacgt
     6661 ttccctttct atgttactgc tcatgcggtg aagttaacgc acgctcactg caggacaaca
     6721 gtaaaatcag agcgtttctg cttttactga tgtctggcgg tcggagctgg tgaccagttt
     6781 gacccatatc tcatggggca gggttttcca ccttgtccgg tattcttact tccccgaaac
     6841 gggtttgcgc ttatgaaatc aatgaatatt gccgccagta gtgaactggt atcccgactt
     6901 tcttctcatc gccgcgtggt ggcgttggga gatactgatt ttacggacgt cgcggcagtc
     6961 gtcattaccg ctgcggatag tcgcagtggc attcttgcgt tgcttaagcg caccggtttt
     7021 catctaccgg tgtttttgta ttccgaacat gctgttgaat tacctgcggg cgttacggcg
     7081 gtaatcaacg gcaacgagca gcagtggctg gagctggaat ccgcagcctg tcagtatgaa
     7141 gagaatttgc tgccaccgtt ttatgacacg ctgacgcagt acgttgagat gggcaacagc
     7201 acctttgctt gccctggaca tcaacatggt gcgttcttta aaaagcatcc tgccggacgc
     7261 catttttacg atttctttgg tgagaacgtc tttcgcgccg atatgtgtaa cgctgacgta
     7321 aaattgggcg atctgcttat tcatgaagga tcggcgaaag atgcgcagaa attcgcagcc
     7381 aaagtctttc atgccgataa aacctatttt gtgctgaacg gcacatcggc agcgaataaa
     7441 gtggtgacga atgcgctgtt aacgcgtggc gatctggtgc tcttcgaccg taacaaccat
     7501 aagtcgaatc atcacggcgc gctgattcag gcggggacga cgccggtcta tctggaagct
     7561 tcacgtaacc cgtttggttt cattggcggt attgatgcgc actgttttaa tgaagagtat
     7621 ctgcgccagc aaattcgcga cgttgcgcca gaaaaagccg acctgccgcg cccgtttcgc
     7681 ctgacgatta ttcagctggg aacctatgac ggcactgtct ataacgcccg tcaggtgatc
     7741 gataccattg ggcatctgtg tgattacatt ctgtttgatt ccgcgtgggt cggttacgaa
     7801 cagtttatct cgatgatggc ggatagctcg ccgctgctgt tagaacttaa cgaaaacgat
     7861 ccggggattt ttgtgaccca gttggtgcac aaacagcagg ctggattctc acagacgtcg
     7921 cagatccata aaaaagataa ccatatccgc ggacaggcgc gtttttgccc gcataagcgg
     7981 ttgaataacg cctttatgct ccatgcttct accagccctt tctatccgct gtttgctgca
     8041 ctggatgtta acgccaaaat tcatgaaggg gagagtgggc gtcggctgtg ggctgagtgc
     8101 gttgagttgg gtattgagtc gcgcaaggct attcttgcgc gctgtaagct gttccgcccg
     8161 tttatcccgc ccgttgttga tggcaaattg tggcaggatt atccgacatc agtgttagcc
     8221 agcgaccgcc gttttttcag ttttgagccg ggggcgaagt ggcacggctt tgaaggatat
     8281 gccgcggatc agtattttgt tgatccgtgc aagctgttac tcactacacc aggtatcaat
     8341 gccgaaaccg gcgaatatag cgactttggc gttccggcga cgattctggc gcactatctg
     8401 cgtgagaacg gcattgtgcc ggagaagtgc gatctcaact ccattctgtt cttattaact
     8461 ccggcggaaa gccacgagaa gctggcacaa ctggtggcga tgctggcgca atttgaacag
     8521 catattgagg atgactcgtc gctggctgag gtgttgccga gcgtttataa caaatatccg
     8581 gtgcgctatc gcgactacac cctgcgccag ttgtgtcagg agatgcacga tctgtatgtc
     8641 agtttcgacg tcaaagacct acaaaaagcg atgttccgcc agcagagttt cccgtcagtg
     8701 gtgatgaacc cccaggatgc gcatagcgct tatattcgcg gtgaagtgga gttggtgcgg
     8761 attcgtgatg ccgaagggcg aattgcggca gaaggggcgt tgccttatcc acctggcgtg
     8821 ctttgcgtgg tacccgggga agtctggggt ggggcggttc aacgttattt ccttgcgctg
     8881 gaagaaggcg tgaatttgtt gccgggattt tcgccggagt tgcaaggtgt ctatagcgaa
     8941 accgatgcgg atggcatgaa acgattgtac ggttatgtgt tgaagtaaga ataaaaaaac
     9001 gggtcacctt ctggcgaccc gtttttcttt gcgtaattag tggctaaccg tctgtgtgcc
     9061 tgtcgggaca cgaacgtgtt tatatttgaa catcgccatg aacgcgaagg ccagaaccac
     9121 ggaataacca gcgaaaatca accatacggt ctgccagtcg gtaatgccgt tttgggtgta
     9181 catctcaaca actttaccgc tcacgatgcc gccgaggata cagccgaagc cgttagtcat
     9241 catcaggaac atcccttgtg cactggcgcg aattgccggg ctaacttctt tttcgacaaa
     9301 caccgaacca gagatgttga agaagtcgaa tgcgcaaccg taaacgatca tcgacagtac
     9361 cagcagtaca gtaccgaacg gagtcgggtc gccgtaagca aacagcgcaa aacgcaggat
     9421 ccacgccaca atactgatca tcattacgtt cttaataccg tagcggctta agaagaacgg
     9481 gatggtcaga atgaacaggg tttcagagat ctgcgaaatc gacatgatga ttgacgcatg
     9541 ctgcacgatg aagctgctgg caaacatcgg atctttgtcg aagctgtgca ggaaggtatt
     9601 accgaacatg ttggtaatct gcagttccgc gcccagcagc attgagaaga tgaagaagat
     9661 tgccatacgc ttgtttttaa acagcgcgaa tgcatcgagg cccagcaggg ttgtccagct
     9721 ctgattcgct tgctgtttag caaccggaat atgcggcaga gtcagggtaa acagaaccag
     9781 aatggcggaa agtgctgcgc caatatacag ctgcatgtgg cttaattcga agccagacag
     9841 gctcaccacc cacattgcca tgataaagcc gatggtgccc cagatacgga ttggcgggaa
     9901 gtcagtaacg atatccatcc cggcattttg caggcgatag taagagatgg tgttgattaa
     9961 cccaagcgtt ggcatataag caaacgagtt aatcaatatc acaaggaaca tcgcttccgg
    10021 cgtcgtgacc tgtgccgcca tgaacagcgt gatagcgcca atggtgtggc aaatggcata
    10081 tacccatttc gcacttaacc atttgtcggc cacaatcccc agcagcgcag gcataaatac
    10141 cgcggcgata cccagtgagc tataaactgc gccaatagaa gcaccgtcaa acttcagggt
    10201 aacaaacata taggagccga gggtcgtcag ccaacttccc cacagacaga actgcagaaa
    10261 agagaggatt ttcagctgca gcttaagatt catgttaatt tcctcacatc gtgatgcgga
    10321 tgagtgtttg aaaaagcaca tttgaccgtg cgagatgatg ggattctatc aacttcgcgg
    10381 actggttttt tgttacctgt ggcaaataat tgcaaacatt cccaatttgc aaaacgaaat
    10441 tgtgatgtgg ataacatttt tgcccctgag catcgtcagg ggcggttaat ggaattatcg
    10501 gcggcggtag gatttttgcg cggtattcac tttataaaga taacggcgag attccgcaga
    10561 gggatggcgg gtcgtcagcg tctgataaac atcgcccggc gtcatggtgt taataatatt
    10621 ggcagcctga atcttatcat tcgaaaagac tcgcagcacg ctgcctgcgc cgccgttata
    10681 ggcggtgatg acggcataac gccgcgatgt tgggttatca attccgccga gataaacatt
    10741 gttcagcatc gccagatacg cggtgccggt atcaatattg ctggcaggat caaacaagaa
    10801 actgcggctc ggcgtgccgg atttcccctg cgagcggaac acatctttcc cggcagtatt
    10861 ttgtaccacc tgcattaatc ccagcgcatc ggaacggctg accgcatacg ggttaaagga
    10921 agattcagtc tgcataattg ccagaatcag cgactcatca acgccatatt tccgtgacgc
    10981 ctggcggacc atgccgagat atttgtgcgc acgtttatca aggtggttcg gcaccatgtt
    11041 aatggtgacg ctgtagatga tacgtagccc gttgctgcgg cttttcagac ggtttttcag
    11101 cagataatcc gcgaagttgc ttgcgcgacc ttcccagcga atcggctgcc cggtgttgtc
    11161 caccacctga ccgtaaagga aaggttcttt cgaaattgta atatcatcaa cgtcggaata
    11221 gagatcgacc gaactcggat cgtcacccat cagcaacgtt ttgataattg cccggcgcaa
    11281 atgcgcggca ggttctgtcc cggcgatggt ttcgatagta atcgtaccgt catcgaagtt
    11341 gatgtggctg cgggtctgat attgatcggt gtatttcacg tagtccttag gaccggcgat
    11401 caccacctct ttgaagcccc agatgttctc aatattgtgg gcaaattgcc ccatcagaat
    11461 atcaaaaccg ttggtatctt tgacccaggc ttcgttatag gtatcgcctt ttttggtcgt
    11521 cgaacaggag atgagcaacg gcgcaatcaa agccagcgcg agatattttt tcatcattcc
    11581 gggtgcgtgt tgtgtttgtt tttatgcaag ctgccggagg cgtgctccgg cactgttttt
    11641 attttttatc ttccggcgta tagccctcga tatgcacctc tttaccctcg aacaggaagt
    11701 tgaccatctc ctgctccagc agcttgcggt gctcggcatt catcatgttg agtttctttt
    11761 cattaatcag catggtttgt ttgtgctgcc actgcgccca ggcttctttg gagatctcgt
    11821 tatagatgcg ttttcccagc tcgccggggt acagctgaaa atcctgacct tctgcttcac
    11881 gttgcaggaa agtacaaaaa atcgttctgc tcataaatca tcctctttat cgactcacgc
    11941 gctaaaccgg cgcgccagtg cgtaactgct gtaacaaacg ctccacggga gccgccaggc
    12001 caactgacgg cggttgcgct aagttatacc agagcgcatt gccttcatcc atgcagccgg
    12061 tgaatgacga cacgggaagc cacataggca caatatctaa gtggaaatgg ctgaaggtat
    12121 ggcgaaacgc ggtcagttgc gtcaggttat cggcagcaat ctgccgttgt gccagccaat
    12181 gccgcaaact ttcttcgtcg gcaaactgcg ggaaacagta taaaccgccc cacaatccgc
    12241 tcggcggacg ctgcgccagc aatacttcat cttcgtgctg taacaggaga aagtagccgg
    12301 tgcgctccgg cagcgtctgt ttcggttttt tgcccggata aagcgaccag ctattgttgg
    12361 tggcggcaat acatccgttt tgtagcggac agagcgaaca tttcggcttc gaacgcgtgc
    12421 aaatcatcgc gcccaaatcc atcatcgcct gattaaaccg ttccacgccg accgcgggcg
    12481 tcacctgctc gctcaaactc catagtttat tctcgacctc ttttttccca ggccagccgc
    12541 ttacagcata gcagcgcgcc agcacgcgtt tgacgttacc gtcgagaatc ggaaagtgct
    12601 tacccagaga aagcgagaga atcgcgcctg cggtggaacg cccgacgccc ggtaacgccg
    12661 cgacttcttc aaaggtttcc gggaatttac caccgtgtaa ggtcgccact tgttgtgccg
    12721 ctttatgcag gttgcgcgcg cgggcgtaat agccaagccc ggtccacaag tggagaactt
    12781 cgtcgagcgg cgcattggcg agatcggtca ccgtcgggaa gtgcgccata aagcgttcaa
    12841 aataggggat aacggtcgca acctgagttt gttgcaacat cacttctgag agccatactt
    12901 tgtagggcgt cttgtcaatt tgccagggca gcgttttccg cccgtattta tcgtaccagt
    12961 ccagaacctg ggctgaaaat tgcgacgctt gcatggtcac cgacttcact gttgttgggg
    13021 gcaagattgc agcacagcga cggcagggtg taaaccggaa ctttccgcag ccacggcctt
    13081 atcatgcttg catccggcaa ctaactttgg ataatgcccg ttttcagaac actttcacaa
    13141 gcgactaaat ctttatgaaa aacgacgtca tttcaccgga atttgatgaa aacggccgcc
    13201 cactgcgccg tatccgtagt tttgtgcgcc gccaggggcg actgaccaaa gtccaggaac
    13261 atgcgctgga aaactactgg ccggtgatgg gcgttgagtt cagtgaagat atgctggatt
    13321 tccccgcgct ttttggccgt gaagcgccgg tgacgcttga gattggtttt ggcatggggg
    13381 cgtcgctggt ggcaatggct aaagatcgcc ccgagcagga cttcctcggc attgaagtgc
    13441 attcaccggg cgttggtgcg tgcctggctt ctgcgcatga agaaggttta agcaacctgc
    13501 gcgtgatgtg tcacgatgcg gttgaagtgc tgcataaaat gattcctgac aattcattgc
    13561 gcatggtgca gctctttttc cctgacccgt ggcacaaagc gcgccataat aaacgccgta
    13621 tcgttcaggt gccgtttgcc gaactggtaa aaagcaaact gcagctgggg ggcgtattcc
    13681 atatggcgac cgactgggaa ccttatgcgg aacatatgct tgaagtgatg tcttctattg
    13741 acggttataa aaacctgtca gagagcaatg attacgtacc gcgtccggca tcacgtccgg
    13801 tgacgaaatt tgaacaacgt ggtcatcgtc ttggtcacgg agtatgggac ttaatgttcg
    13861 agagggtgaa ataatggcaa agaaccgtag ccgtcgtctg cgtaaaaaaa tgcacatcga
    13921 cgaattccag gaattaggat tttcggtggc atggcgattc ccggaaggta catcggaaga
    13981 acagattgat aaaatcgttg atgactttat taacgaggtt atcgaaccga acaaactggc
    14041 ctttgacggc agcggttatc tggcctggga aggtctgatc tgcatgcagg aaatcggcaa
    14101 atgcaccgaa gaacatcagg cgattgtgcg taagtggctg gaagagtgca aactggatga
    14161 ggtacgcacc agcgaacttt tcgacgtttg gtgggactaa gaaagcatac gggcgatgac
    14221 aaatgcaaaa ctgcctgatg cgctacgctt atcaggccta cagagatgca cgatcgagta
    14281 ggcgggataa ggcgtttacg ccgcatccgg catggaaaac gcgtactttg ttatcaatct
    14341 ggggccagca tatgctggcc tgatttgttc ttgagggaag actatgatgc gcaaaatgct
    14401 gctggcggca gcactttcag tgacggcaat gaccgctcac gccgactacc agtgcagcgt
    14461 cacgccgcgt gacgatgtga ttgtcagccc gcaaaccgtg caggtgaagg gcgaaaacgg
    14521 caatctggtg atcacgccag acggcaacgt gatgtataac ggtaagcaat attccctgaa
    14581 tgccgcccag cgcgagcagg cgaaggatta tcaggctgaa ctacgtagca cgctgccgtg
    14641 gattgatgaa ggcgcgaaaa gccgcgtcga gaaagcccgt attgcgctgg ataaaattat
    14701 cgttcaggag atgggcgaca gcagcaaaat gcgcagccgt ctgaccaaac ttgatgcgca
    14761 gcttaaagag cagatgaatc gcattatcga aacgcgcagc gatggcctga cgttccacta
    14821 taaagccatt gatcaggttc gcgccgaagg ccagcaacta gtgaatcagg caatgggcgg
    14881 aattttacag gacagcatta atgaaatggg cgcgaaagcg gtgctgaaaa gcggcggtaa
    14941 cccattacag aatgtgctgg gaagcttggg cggtctgcaa tcctcaatcc aaaccgagtg
    15001 gaaaaagcag gaaaaagatt tccagcagtt tggcaaagat gtttgtagcc gcgttgtgac
    15061 tctggaagat agccgcaaag ccctggtcgg gaatttaaaa taatcctcta ttttaagacg
    15121 gcataatact tttttatgcc gtttaattct tcgttttgtt acctgcctct aactttgtag
    15181 atctccaaaa tatattcacg ttgtaaattg tttaacgtca aatttcccat gcaaagctaa
    15241 gggataatgc gtagcgtcca cgtaactgga ggaatgaaat ggagtttttc aaaaagacgg
    15301 cacttgccgc actggttatg ggttttagtg gtgcagcatt ggcattaccc aatatcacca
    15361 ttttagcaac cggcgggacc attgccggtg gtggtgactc cgcaaccaaa tctaactaca
    15421 cagcgggtaa agttggcgta gaaaatctgg ttaatgcggt gccgcaacta aaagacattg
    15481 cgaacgttaa aggcgagcag gtagtgaata tcggctccca ggacatgaac gataatgtct
    15541 ggctgacact ggcgaaaaaa attaacgccg actgcgataa aaccgacggc ttcgtcatta
    15601 cccacggtac cgacacgatg gaagaaactg cttacttcct cgacctgacg gtgaaatgcg
    15661 acaaaccggt ggtgatggtc ggcgcaatgc gcccgtccac gtccatgagc gcagacggtc
    15721 cattcaacct gtataacgcg gtagtgaccg cagctgataa agcctccgct aatcgtggcg
    15781 tgctggtggt gatgaacgac accgtactgg acggtcgcga tgtcaccaaa accaacacca
    15841 ccgacgtagc gaccttcaag tctgttaact acggtcctct gggatacatt cacaacggta
    15901 agattgatta ccaacgtacc ccggcacgta agcacaccag cgatacgcca ttcgatgtct
    15961 ctaagctgaa tgagctgccg aaagtcggca tcgtttataa ctacgctaac gcatccgatc
    16021 ttccggctaa agcactggta gatgcgggct atgatggcat cgttagcgct ggtgtgggta
    16081 atggtaacct gtataaatcc gtgttcgaca ccctggcaac cgccgcgaaa aacggcactg
    16141 cagtagtgcg ttcttcccgc gtaccgacgg gtgctaccac tcaggatgct gaagtggatg
    16201 atgcgaaata cggcttcgtc gcctctggca cgctgaaccc gcaaaaagcg cgcgtcctgc
    16261 tgcagctggc tctgacgcaa accaaagatc cgcagcagat ccagcagatt ttcaatcagt
    16321 actaatcacc tcgtcccggc atcgtgccgg ggctttttca cttcagactt acgtccattg
    16381 ccaattttca ttaccctaat gataatcacc ggaataaatt attccgcgcg agggttttcg
    16441 ggtgaaaaag caatggattg ttggcacggc gctgcttatg ttgatgactg gtaatgcctg
    16501 ggcagatggc gaaccgccaa ctgaaaatat cttaaaagat caattcaaaa agcagtatca
    16561 cggcattctc aagcttgatg ccatcagctt aaaaaatctt gatgctaagg gtaatcaggc
    16621 cacctggtca gcggaaggcg atgtctcttc cagtgacgat ctctatacct gggtcggtca
    16681 gttggcagat tacgaactgc tcgaacagac ctggacgaaa gataaaccgg taaaattctc
    16741 ggcgatgtta accagtaaag gaacgcccgc gtctggctgg tcggtgaact tttactcttt
    16801 tcaggcggca gccagcgatc gtgggcgggt ggtcgacgat atcaaaacga ataataaata
    16861 tctgatcgtg aatagcgaag atttcaatta tcgctttagt cagcttgaat ctgcgttgaa
    16921 tacccagaag aactcgattc ctgcgctgga aaaggaagtg aaagcgcttg ataagcaaat
    16981 ggtggcggcg cagaaagcag cggatgcata ttggggaaaa gatgcaaacg gtaagcaaat
    17041 gacccgcgaa gaagccttta aaaaaattca ccaacagcgt gatgagttta ataaacagaa
    17101 cgatagcgag gcgttcgcgg ttaaatatga caaagaggtt tatcaaccgg cgattgcggc
    17161 atgtcataaa cagagtgaag agtgttatga agtgccgatt cagcagaaac gagatttcga
    17221 tatcaacgaa caacggcgac agacttttct gcaatcacaa aaactcagcc gtaaattgca
    17281 ggatgactgg gtaacgctcg aaaaagggca atatccgtta acgatgaaag tttcagaaat
    17341 aaacagtaaa aaagtcgcga ttttgatgaa aattgacgat atcaaccaag ctaatgaacg
    17401 ctagaaaaaa gataccgaac aactgcggcg caatggggtg attaagtaac tcatgttaag
    17461 tgcctttgcc ggataatata aaaagttatc cggcaaatta ttttttacat tagccctagt
    17521 agctgtggaa tgaacaagga tatttgcgga atgtaagtga tcatcatcaa cgctactagc
    17581 aatgcagcat aaaatagcag caatggtttg attaaatgtt gaatcttcac acccgagata
    17641 gaacaaccaa caaatagcgc actacccacc ggcggtgtta acagcccaat acaaaggttg
    17701 gccaccatca taataccgaa atgtactggg tccattccca gctcttgtgc aatcggcaag
    17761 aagatcggtg taaagattag tacagccggt gtcatgtcca taaagatgcc gacaattaat
    17821 aagacgatat taatcagcag caatatgatc agtgggttat cagaaatccc catcaatgcg
    17881 tcgctgatca tataaggaat atcagcatta gtcattgccc aggacatgcc cacggaaaaa
    17941 ccgatcaaca gtaatacaat cgaagtggta acaaccgact ccagaatcag tttgggtaaa
    18001 tggcgccatt ttacttcccg gtaaatcagc accgagagaa taaatgtgta gacaacggca
    18061 attgctgacg cttcagttgc ggtaaaaatg ccgcccagaa tgccgcccat cacaatgaac
    18121 accagaagca aacttggtaa ggcatccaga aacgctttac aggcctgagc aaaggttgct
    18181 ctttctgaaa gcgggtagcc acggcgttta gcaataatgc cgcagactat cattaccgca
    18241 agacccatta agatccctgg caaatatccc gccataaaca gcgatgctac agaaacgccg
    18301 ccagctgtca gggaaaacac aattaacacg tttgagggcg gtatcagtaa tccagtaata
    18361 caagatgaga cgtttacagc ggtagaaaat gccggatcat aaccttcttt ggtctgaatg
    18421 ggattcagtg tgccaccaac ggcagcagct gccgcaacgg ctgaaccaga aatggaacca
    18481 aacatcatat tcgccaggac gttcacatga ccaagcgaac caggaacgcg gccaaccatt
    18541 acctgggcga ggttaatcaa acgtatcgca ataccgccgc tgttcatcag tgttccggca
    18601 aagatgaaaa aggggattgc caacagggca aagttatcca gtccattagc cagtcgttgc
    18661 gaaacagtga ttgccgcgat atcccaggga aacatcaaaa gcattgaagc tacagtggca
    18721 ataccgattg agaacgaaat gggcacacca ataaagacca gcacaaaaaa acagccaaac
    18781 agtgtaatcg cgatataact atc
//
LOCUS       DAJTRC010000078        18051 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.078, whole genome shotgun sequence.
ACCESSION   DAJTRC010000078 DAJTRC010000000
VERSION     DAJTRC010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 18051)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18051)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18051
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.078"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            446..1129
                     /locus_tag="OO357_002881"
     CDS             446..1129
                     /locus_tag="OO357_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001049551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3251 domain-containing protein"
                     /protein_id="HCR5887760.1"
                     /translation="MNLKKIFFSAVTVSVLCALTGCDYIEEGKPESSLLKQQEEHNNK
                     IVLLEKQQAQLKSQLETIQKQQTGIINSTKTLTHVIKSVKDQQNTFIFTEFNPAKTKY
                     FILNNGSVALAGRVLSIDATENGSVIHISLVNLLSTPISNIGFNATWGGEKPVDAKEF
                     ARWQQLLFNTSMKSTLKLLPGQWQDINLTLKGVSPNNLGYLKLAINMENIQFDNLPSA
                     ENRQKRSKK"
     gene            1173..5835
                     /locus_tag="OO357_002882"
                     /pseudo
     CDS             1173..5835
                     /locus_tag="OO357_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
     gene            6203..7858
                     /gene="lldP"
                     /locus_tag="OO357_002883"
     CDS             6203..7858
                     /gene="lldP"
                     /locus_tag="OO357_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HCR5887761.1"
                     /translation="MNLWQQNYDPAGNIWLSSLIASLPILFFFFALIKLKLKGYVAAS
                     WTVAIALAVALLFYKMPVANALASVVYGFFYGLWPIAWIIIAAVFVYKISVKTGQFDI
                     IRSSILSITPDQRLQMLIVGFCFGAFLEGAAGFGAPVAITAALLVGLGFKPLYAAGLC
                     LIVNTAPVAFGAMGIPILVAGQVTGIDSFEIGQMVGRQLPFMTIIVLFWIMAIMDGWR
                     GIKETWPAVVVAGGSFAIAQYLSSNFIGPELPDIISSLVSLLCLTLFLKRWQPVRVFR
                     FGDLGASQVDMTLAHTGYTAGQVLRAWTPFLFLTATVTLWSIPPFKALFASGGALYEW
                     VINIPVPYLNKLVARMPPVVSEATAYAAVFKFDWFSATGTAILFAALLSIVWLKMKPS
                     DAISTFGSTLKELALPIYSIGMVLAFAFISNYSGLSSTLALALAHTGHAFTFFSPFLG
                     WLGVFLTGSDTSSNALFAALQATAAQQIGVSDLLLVAANTTGGVTGKMISPQSIAIAC
                     AAVGLVGKESDLFRFTVKHSLIFTCMVGVITTLQAYVLTWMIP"
     gene            7858..8634
                     /gene="lldR"
                     /locus_tag="OO357_002884"
     CDS             7858..8634
                     /gene="lldR"
                     /locus_tag="OO357_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LldR"
                     /protein_id="HCR5887762.1"
                     /translation="MIVLPRRLSDEVADRVRALIDEKNLEAGMKLPAERQLAMQLGVS
                     RNSLREALAKLVSEGVLLSRRGGGTFIRWRHDTWSEQNIVQPLKTLMADDPDYSFDIL
                     EARYAIEASTAWHAAMRATPGDKEKIQLCFEATLSEDPDIASQADVRFHLAIAEASHN
                     IVLLQTMRGFFDVLQSSVKHSRQRMYLVPPVFSQLTEQHQAVIDAIFAGDADGARKAM
                     MAHLSFVHTTMKRFDEDQARHARITRLPGEHNEHSREKNA"
     gene            8631..9821
                     /gene="lldD"
                     /locus_tag="OO357_002885"
     CDS             8631..9821
                     /gene="lldD"
                     /locus_tag="OO357_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent L-lactate dehydrogenase"
                     /protein_id="HCR5887763.1"
                     /translation="MIISAASDYRAAAQRILPPFLFHYMDGGAYSEYTLRRNVEDLSE
                     VALRQRILKNMSDLSLETTLFNEKLSMPVALAPVGLCGMYARRGEVQAAKAADAHGIP
                     FTLSTVSVCPIEEVAPAIKRPMWFQLYVLRDRGFMRNALERAKAAGCSTLVFTVDMPT
                     PGARYRDAHSGMSGPNAAMRRYLQAVTHPQWAWDVGLNGRPHDLGNISAYLGKPTGLE
                     DYIGWLGNNFDPSISWKDLEWIRDFWDGPMVIKGILDPEDARDAVRFGADGIVVSNHG
                     GRQLDGVLSSARALPAIADAVKGDIAILADSGIRNGLDVVRMIALGADTVLLGRAFLY
                     ALATAGQAGVANLLNLIEKEMKVAMTLTGAKSISEITQDSLVQGLGKELPTALAPMAK
                     GNAA"
     gene            10017..10490
                     /gene="trmL"
                     /locus_tag="OO357_002886"
     CDS             10017..10490
                     /gene="trmL"
                     /locus_tag="OO357_002886"
                     /EC_number="2.1.1.-"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HCR5887764.1"
                     /translation="MLNIVLYEPEIPPNTGNIIRLCANTGFRLHIIEPMGFAWDDKRL
                     RRAGLDYHEFTAVTRHHDYRAFLEAENPQRLFALTTKGTPAHSAVSYQDGDYLMFGPE
                     TRGLPASILDALPAEQKIRIPMVPDSRSMNLSNAVSVVVYEAWRQLGYPGALLRD"
     gene            complement(10543..11364)
                     /gene="cysE"
                     /locus_tag="OO357_002887"
     CDS             complement(10543..11364)
                     /gene="cysE"
                     /locus_tag="OO357_002887"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001112113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HCR5887765.1"
                     /translation="MSCEELEIVWNNIKAEARTLADCEPMLASFYHATLLKHENLGSA
                     LSYMLANKLSSPIMPAIAIREVVEEAYAADPEMIASAACDIQAVRTRDPAVDKYSTPL
                     LYLKGFHALQAYRIGHWLWNQGRRALAIFLQNQVSVTFQVDIHPAAKIGRGIMLDHAT
                     GIVVGETAVIENDVSILQSVTLGGTGKSGGDRHPKIREGVMIGAGAKILGNIEVGRGA
                     KIGAGSVVLQPVPPHTTAAGVPARIVGKPDSDKPSMDMDQHFNGINHTFEYGDGI"
     gene            complement(11444..12463)
                     /gene="gpsA"
                     /locus_tag="OO357_002888"
     CDS             complement(11444..12463)
                     /gene="gpsA"
                     /locus_tag="OO357_002888"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HCR5887766.1"
                     /translation="MNQRNASMTVIGAGSYGTALAITLARNGHEVVLWGHDPEHIATL
                     ERDRCNAAFLPDVPFPDTLHLESDLATALAASRNILVVVPSHVFGEVLRQIKPLMRPD
                     ARLVWATKGLEAETGRLLQDVAREALGDQIPLAVISGPTFAKELAAGLPTAISLASTD
                     QTFADDLQQLLHCGKSFRVYSNPDFIGVQLGGAVKNVIAIGAGMSDGIGFGANARTAL
                     ITRGLAEMSRLGAALGADPATFMGMAGLGDLVLTCTDNQSRNRRFGMMLGQGMDVQSA
                     QEKIGQVVEGYRNTKEVRELAHRFGVEMPITEEIYQVLYCGKNAREAALTLLGRARKD
                     ERSSH"
     gene            complement(12463..12930)
                     /gene="secB"
                     /locus_tag="OO357_002889"
     CDS             complement(12463..12930)
                     /gene="secB"
                     /locus_tag="OO357_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015369180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HCR5887767.1"
                     /translation="MSEQNNTEMTFQIQRIYTKDISFEAPNAPHVFQKDWQPEVKLDL
                     DTASSQLADDVYEVVLRVTVTASLGEETAFLCEVQQGGIFSIAGIEGTQMAHCLGAYC
                     PNILFPYARECITSMVSRGTFPQLNLAPVNFDALFMNYLQQQAGEGTEEHQDA"
     gene            complement(12993..13244)
                     /gene="grxC"
                     /locus_tag="OO357_002890"
     CDS             complement(12993..13244)
                     /gene="grxC"
                     /locus_tag="OO357_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HCR5887768.1"
                     /translation="MANVEIYTKETCPYCHRAKALLSSKGVSFQELPIDGNAAKREEM
                     IKRSGCTTVPQIFIDAQHIGGCDDLYALDARGGLDPLLK"
     gene            complement(13386..13817)
                     /locus_tag="OO357_002891"
     CDS             complement(13386..13817)
                     /locus_tag="OO357_002891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HCR5887769.1"
                     /translation="MQEIMQFVGRHPILSIAWIALLVAVLVTTFKSLTSKVKVITRGE
                     ATRLINKEDAVVVDLRQRDDFRKGHIAGSINLLPSEIKANNVGELEKHKDKSVIVVDG
                     SGMQCQEPANALTKAGFAQVFVLKEGVAGWAGENLPLVRGK"
     gene            14062..15606
                     /gene="gpmM"
                     /locus_tag="OO357_002892"
                     /gene_synonym="pgmI"
     CDS             14062..15606
                     /gene="gpmM"
                     /locus_tag="OO357_002892"
                     /gene_synonym="pgmI"
                     /EC_number="5.4.2.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-independent
                     phosphoglycerate mutase"
                     /protein_id="HCR5887770.1"
                     /translation="MSVSKKPMVLVILDGYGYREEQQDNAIFSAKTPVMDALWANRPH
                     TLIDASGLEVGLPDRQMGNSEVGHVNLGAGRIVYQDLTRLDVEIKDRAFFANPVLAGA
                     VHKAKNAGKAVHIMGLLSAGGVHSHEDHIMAMVELAAERGAEKIYLHAFLDGRDTPPR
                     SAESSLKKFEEKFAALGKGRVASIIGRYYAMDRDNRWDRVEKAYDLLTLAQGEFQADT
                     AVAGLQAAYARDENDEFVKATVIRAEGQPDAAMEDGDALIFMNFRADRAREITRAFVN
                     ADFDGFARKKVVNVDFVMLTEYAADIKTAVAYPPASLVNTFGEWMAKNDKTQLRISET
                     EKYAHVTFFFNGGVEESFKGEDRILINSPKVATYDLQPEMSSAELTEKLVAAIKSGKY
                     DTIICNYPNGDMVGHTGVMEAAVKAVEALDHCVEEVAKAVESVGGQLLITADHGNAEQ
                     MRDPATGQAHTAHTNLPVPLIYVGDKNVKAVAGGKLSDIAPTMLSLMGMEIPQEMTGK
                     PLFIVE"
     gene            15616..16899
                     /gene="envC"
                     /locus_tag="OO357_002893"
     CDS             15616..16899
                     /gene="envC"
                     /locus_tag="OO357_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC"
                     /protein_id="HCR5887771.1"
                     /translation="MRGKAINTMTRAVKPRRFAIRPIIYASVLSAGVLLCAFSAHADE
                     RDQLKSIQADIAAKERAVRQKQQQRASLLAQLKKQEEAISEATRKLRETQNTLNQLNK
                     QIDEMNASIAKLEQQKAAQERSLAAQLDAAFRQGEHTGIQLILSGEESQRGQRLQAYF
                     GYLNQARQETIAQLKQTREEVTMQHAELEEKQSEQQTLLYEQRAQQAKLTQALNERKK
                     TLAGLESSIQQGQQQLSELRANESRLRNSIARAEAAAKARAEREAREAQAVRERQKEA
                     TRKGTTYKPTESEKSLMSRTGGLGAPRGQAFWPVRGPTLHRYGEQLQGELRWKGMVIG
                     ASEGTEVKAIADGRVILADWLQGYGLVVVVEHGKGDMSLYGYNQSALVSVGSQVRAGQ
                     PIALVGSSGGQGRPSLYFEIRRQGQAVNPQPWLGR"
     gene            16903..17862
                     /locus_tag="OO357_002894"
     CDS             16903..17862
                     /locus_tag="OO357_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312519.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HCR5887772.1"
                     /translation="MFPFRRNVLAFAALLALSSPVLAGKLAIVIDDFGYRPHNENQVL
                     AMPSAISVAVLPDSPHAREMATKAHNSGHEVLIHLPMAPLSKQPLEKNTLRPEMSSDE
                     IERIIRSAVNNVPYAVGINNHMGSKMTSNLFGMQKVMQALERYNLYFLDSVTIGNTQA
                     MRAAQGTGVKVIKRKVFLDDSQNEADIRVQFNRAIDLARRNGSTIAIGHPHPSTVRVL
                     QQMVYNLPPDITLVKASSLLNEPQVDTSTPPKNAVPDAPRNPFRGVKLCKPKKPIEPV
                     YANRFFEVLSESISQSTLIVYFQHQWQGWGKQPEAAKFNASAN"
     gene            complement(17849..18051)
                     /locus_tag="OO357_002895"
                     /pseudo
     CDS             complement(17849..18051)
                     /locus_tag="OO357_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418072.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
BASE COUNT         4547 a         4664 c         4674 g         4166 t
ORIGIN      
        1 aattcactcc ctttacttcg atttaccgct ggttgatatt ctgaaaatgc atttttaccg
       61 aaatagccat ctataaacaa taaaaatata tagaagatat catttgttaa ttaacaagca
      121 tttacgtctc tttttttcgg atttaattgt gaaattttaa tcatcatatt cacaaaatca
      181 attatctgtg aaatttttcc cagacatggt ttctttcata tttgacattt attaataatc
      241 atcattagaa tccacgaaaa ataatatatt catacaaaca cagacatctt ttgaaacgaa
      301 tgatacattt ttatgatgaa tgcttaaaac aatatttaat cgacacacat cactgtcatt
      361 aatgtaaagc aacatagctc accaataata caatggacaa ctctaccggc attgagcgct
      421 ggtcggagga aataaggatt tttacatgaa cttgaagaag atttttttca gtgctgtcac
      481 tgtttcagtt ctctgcgcat taacgggatg tgactacatt gaagaaggca agccagaaag
      541 tagtttactg aaacaacaag aagaacataa taataaaatt gtgctacttg aaaaacaaca
      601 agcacagcta aagagtcaac tcgaaactat tcaaaaacaa caaactggca taataaacag
      661 tacaaagact ttaactcacg taattaaatc cgttaaagac caacaaaata cttttatttt
      721 taccgagttt aacccagcaa aaacgaagta tttcatcctg aacaacggtt cggtggcttt
      781 agcgggtcga gtgttatcca ttgacgccac agagaatggt agtgttattc atatctcact
      841 ggtcaactta ttaagtacac ctatctcaaa cattggtttt aatgcgacat ggggtggtga
      901 aaaacctgtc gatgccaaag agtttgcccg gtggcaacaa ttacttttca acacatcaat
      961 gaaatccaca ttgaaattat taccaggtca atggcaagac attaatttga ccctgaaggg
     1021 tgtatcgccc aataacctgg gatatctgaa gttagccatc aacatggaaa atattcagtt
     1081 cgacaatctt ccctctgctg aaaatcggca gaaaagaagc aaaaaataaa tgcagttagt
     1141 acgcttataa caaggaatag cgagcatcat taatgaacaa aatatttaaa gttatctgga
     1201 accctgcgac agggaattat actgttacca gcgaaacggc aaaaagccgt ggcaagaaat
     1261 ctgggcgcag taagctgtta atttctgcgc tggttgcggg tggaatgttg tcgtcgtttg
     1321 gggcattggc gaatgccggg aatgacaacg gtcagggtgt tgattacggt agtggatcag
     1381 ctggcgacgg ctgggttgct ataggcaaag gggcgaaagc aaatactttt atgaacacca
     1441 gtggttccag tactgctgtg ggttatgacg ctatagctga aggccaatat agctctgcca
     1501 tcgggtcaaa aacccatgcg attggtggtg catcaatggc ctttggggtt agtgcaatat
     1561 cagaaggcga tagaagtata gcactgggtg cctcttcgta ttcattgggc caatactcaa
     1621 tggccctcgg ccgttattca aaagcattgg gtaaattgtc tattgctatg ggggactctt
     1681 ccaaagcgga aggagcaaac gccattgccc tggggcgtag cagtgtagct agcggtactg
     1741 acagcctcgc atttggcaga caatcacttg ccagcgcagc gaacgctatt gcgataggtg
     1801 ctgagaccga agccgctgaa aatgcaactg ctattggcaa taatgcgaag gcaaaaggga
     1861 ctaatagcat ggcaatgggg ttcggaagcc ttgccgataa agtcaatact atcgcattag
     1921 gaaatggcag ccaggctctg gcagataatg caatcgccat aggccagggc aacaaagctg
     1981 atggcgtgga tgccatcgct ctgggtaatg gtagccagtc gagaggctta aacaccattg
     2041 ccttaggcac agccagtaat gcaactggtg ataagagtct tgcgcttggt agtaatagca
     2101 gtgccaacgg tattaactct gtcgcgctgg gcgcagattc cattgcggat ttagacaata
     2161 ccgtctctgt cggcaatagt tcattaaaac gcaagatcgt taatgtgaaa aatggcgcga
     2221 tcaagtctga cagttacgat gccattaatg gttcacagct ttatgccatt agcgactcgg
     2281 tagcaaaaaa ggcttggagg aggggctgca gtagatgttg atgacggtac tgttacagca
     2341 ccaacctaca atttaaaaaa tggtagcaaa aataacgtag gggctgcgct cgctgtactt
     2401 gatgaaaaca ccctgcaatg ggaccaaacc aaaggcaaat acagcgctgc tcatggtact
     2461 agtagcccaa ctgccagcgt aatcaccgat gttgcggatg gcacgatttc agcctccagt
     2521 aaggatgcgg ttaacggttc ccaactgaaa gctaccaatg acgatgtcga agccaacacc
     2581 accaatatca ccaacctgac ggattccgtt ggtgaccttc aggctgatgc cctgctctgg
     2641 aacgaaacta aaaaggcatt cagtgcagct cacggccagg ataccaccag caaaatcacc
     2701 aacgttaaag atgccgacct gacggctgac agcactgatg ctgttaacgg ctctcagctg
     2761 aaaaccacca acgatgctgt ggcgacgaat accaccaata tcgccaataa cacttccaat
     2821 attgccacta acaccaccaa catctctaac ctgactgaga cggtgactaa tcttggtgag
     2881 gatgcgctga aatgggataa ggacaatggt gtattcacgg cagctcatgg caccgagacc
     2941 accagcaaaa taaccaacgt taaagatggc gacctgacga ctggcagcac cgatgccgtt
     3001 aacggctctc agctgaaaac caccaacgat gccgtggcga cgaataccac caatatcgcc
     3061 actaacacca ccaacatctc taatctgact gagacggtga ctaatcttgg tgaggatgcg
     3121 ctgaaatggg ataaggacaa tggtgtcttc actgcagctc atggcaacaa taccgccagc
     3181 aaaatcacca atatcctgga cggcacagtc actgcaacca gttccgatgc cattaacggt
     3241 agccagcttt atgacttaag cagcaatatc gccacctact tcggcggcaa tgcttctgtg
     3301 aatactgacg gtgtgtttac cggtccaacc tacaaaatcg gtgaaacaaa ttattataac
     3361 gtcggcgatg cactggctgc gattaactcc tcatttagca cgtctctcgg cgatgctctg
     3421 ctttgggatg ccaccgcagg taaattcagt gccaaacacg gtactaatgg tgacgcaagc
     3481 gtgatcactg atgtcgcaga tggtgaaatt tcagactcca gttctgacgc agtaaacggc
     3541 tcacaactcc acggcgtgag cagttatgtt gttgatgcgc tggggggtgg tgccgaagtc
     3601 aatgcagacg gcaccatcac tgcgccgacg tacaccattg ctaatgctga ttacgataat
     3661 gtcggtgatg ccctgaatgc tatcgatacc actcctgacg acgctctgct ctgggatgcg
     3721 gacgccggtg aaaatggtgc atttagcgcc gctcacggaa aagataaaac tgccagtgta
     3781 atcactaacg tcgctaacgg tgcaatctct gctgccagca gcgacgcgat taacggctca
     3841 caactctata ccaccaataa gtacatcgct gatgcgctgg gtggtgacgc agaagtcaac
     3901 gctgacggca ccatcaccgc accgacttac accattgcga acgccgagta caacaacgtc
     3961 ggtgacgccc tggatgcgct tgatgataac gccctgctgt gggataagac tgccaatggc
     4021 ggtgctggag cctacaatgc cagccatgac ggtaaagcca gcatcatcac taatgtcgct
     4081 aatggcagta ttagtgagga cagtaccgat gcagtgaacg gttctcagtt gaatgcgacg
     4141 aatatgatga ttgagcagaa cacccaaatt atcaatcagc tcgctggtaa caccgacgca
     4201 acctatatcg aagaaaatgg cgctggtatc aactacgttc gtactaacga caacgattta
     4261 gcctttaacg atgcaagcgc ctctggtgtc ggcgctacag ctgtaggtta taacgctgtc
     4321 gcgtctggtg ccagcagcgt agccattggt cagaacagca gcagcaccgt tgataccggt
     4381 attgcgctgg gtagcagctc cgtttccagc cgtgtgattg ccaaaggttc tcgtgacact
     4441 agcgtaacgg aaaatggcgt ggtgattggt tatgacacca ctgacggcga actgctaggt
     4501 gcattgtcaa ttggtgatga cggtaaatac cgccaaatca tcaacgtagc tgatggttca
     4561 gaagcccatg acgccgttac ggttcgccag ttgcagaacg ctattggagc ggtcgccact
     4621 acgccaacca agtactttca cgccaactca acggcagaag actcactggc cgttggtgaa
     4681 gactcgctgg caatgggtgc gaaaactgtc gttaatggta atgcaggtat tggtatcggt
     4741 ttgaacactc tggttctggc tgatgcgatc aacggcattg ctatcggcag taacgcacgc
     4801 gcaaaccatg caaacagtat cgcaatgggt aacggttctc agaccacccg tggtgcacag
     4861 actggctaca ccgcctacaa catggacgca ccgcagaact ctgtaggtga gttttctgtc
     4921 ggtagtgaag acggtcaacg tcagatcaca aacgtcgcag ctggttcagc ggataccgat
     4981 gcggttaacg tgggtcagtt gaaagtcact gatgagcgcg tagcgcaaaa tacccagagc
     5041 attactaacc tgaacaatca ggtcactaat ctggatactc gcgttactaa tatcgaaaac
     5101 ggtattggcg acattgtcac caccggtagc accaagtact tcaagaccaa caccgatggc
     5161 gtagatgcca acgcccaggg taaagatagc gttgctattg gttctggttc cattgctgcc
     5221 gctgacaaca gcgtcgcact gggtaccggt tccgttgcag aggaagaaaa tacaatctct
     5281 gtaggttctt ctactaacca acgccgtatc accaacgtag ctgccagtgt taatgccacc
     5341 gatgcggtta acgtgtcgca gctgaaatct tctgaagcag gcggagtgcg ctacgacacc
     5401 aaagctgatg gttctatcga ctatagcaat atcaccctcg gtggtggcaa cggcagtacg
     5461 actcgtatca gcaacgtctc cgctggcgtc aacaacaacg acgcggtgaa ctacgcgcag
     5521 ttgaagcaaa gcgcgcagga aacgaagcaa tacaccgatc agcggatggt tgagatggat
     5581 aacaaactgt ctaaaactga aagcaagttg agcggtggta tcgcttctgc aatggcaatg
     5641 accggtctgc cgcaggctta tacaccgggt gccagcatgg cttctattgg tggcggtact
     5701 tacaacggtg aatcggcagt tgctttaggt gtatcgatgg tgagcgccaa tggtcgttgg
     5761 gtctacaaat tacaaggtag taccaatagc cagggtgaat actccgccgc actcggtgcc
     5821 ggtattcagt ggtaatgatc cattaagtta gtgtgactaa gggcaaacgc caaattgcct
     5881 gatgcgttac gcgcatcagg caagataagg cgctccgggt caacaatcta agggcaattc
     5941 tctgatgagg attgcccttt tctttaccag acatctcccc ccacaagaat tggccctacc
     6001 aattcttcgc ttatctgacc tctggttcac aattccccaa ttaaaactca catcaatatt
     6061 gccaatacat aacatttagt taaccattca ttgtcattat ccctacacaa cacaattggc
     6121 agtgccactt ttacacaacg tgtgacaagg agatgagcaa cagactcatt acacgatgtg
     6181 cgtggactct aggagacctg caatgaatct ctggcaacaa aactacgatc ccgccgggaa
     6241 tatctggctt tccagtctga tagcatcgct tcccatcctg tttttcttct ttgcgctgat
     6301 taagctcaaa ctgaaaggat acgtcgccgc ctcatggacg gtagcaatcg cccttgccgt
     6361 ggctttgctg ttctataaaa tgccggtcgc taacgcgctg gcctcggtgg tttatggctt
     6421 cttctatggt ttgtggccca tcgcgtggat cattattgca gcagtgttcg tctataagat
     6481 ctcggtgaaa accgggcagt ttgacatcat tcgctcgtct attctttcga taacccctga
     6541 ccagcgtctg caaatgctga tcgtcggttt ctgtttcggc gctttccttg aaggagccgc
     6601 aggctttggc gcaccggtag caattaccgc cgcattgctg gtcggcctgg gttttaaacc
     6661 gctgtacgcc gccggactgt gcctgattgt taacaccgcg ccagtggcat ttggcgcgat
     6721 gggcattcca atcctggttg ccggacaagt aacaggcatc gacagctttg agattggcca
     6781 gatggtgggg cgtcagctac cgtttatgac cattatcgtg ctgttctgga tcatggcgat
     6841 tatggacggc tggcgcggta tcaaagagac gtggcctgcg gtcgtggttg caggcggctc
     6901 gtttgccatc gctcagtacc ttagctctaa cttcattggg ccggagctgc cggacattat
     6961 ctcttcgctg gtatcactgc tctgcctgac gctgttcctc aaacgctggc aaccagttcg
     7021 cgtattccgc ttcggtgact tgggggcgtc acaggttgat atgacgctgg cccacaccgg
     7081 ttacaccgcg ggtcaggtgt tacgtgcctg gacaccgttc ctgttcctga ccgctaccgt
     7141 aacactgtgg agcatcccgc cgtttaaagc cctgttcgca tcgggtggtg cgctgtatga
     7201 gtgggtgatc aacattccgg tgccgtacct caataaactg gttgcccgta tgccaccagt
     7261 ggtcagcgag gctacagcct atgccgccgt gtttaagttt gactggttct ctgccaccgg
     7321 caccgccatt ctgtttgctg cactgctctc gattgtctgg ctgaagatga aaccgtctga
     7381 cgctatcagc accttcggca gcacgctgaa agaactggct ctacccatct actccatcgg
     7441 catggtgctg gcgttcgcct ttatctcgaa ctattccgga ctgtcatcaa cactggcact
     7501 ggcactggcg cacaccggtc atgcattcac cttcttctcg ccgttcctcg gctggctggg
     7561 ggtattcctg accgggtcgg atacctcatc taacgccctg ttcgccgcgc tgcaagccac
     7621 cgcagcacaa caaattggcg tctctgatct gttgctggtt gccgccaata ccaccggtgg
     7681 cgtcaccggt aagatgatct ccccgcaatc tatcgctatc gcctgtgcgg cggtaggcct
     7741 ggtgggcaaa gagtctgatt tgttccgctt tactgtcaaa cacagcctga tcttcacctg
     7801 tatggtgggc gtgatcacca cgcttcaggc ttatgtctta acgtggatga ttccttaatg
     7861 attgttttac ccagacgcct gtcagacgag gttgccgatc gtgtgcgggc gctgattgat
     7921 gaaaaaaacc tggaagcggg catgaagttg cccgctgagc gccaactggc gatgcaactc
     7981 ggcgtatcac gtaattcact acgcgaggcg ctggcaaaac tggtgagcga aggcgtgctg
     8041 ctcagtcgac gcggcggcgg gacgtttatt cgctggcgtc atgacacatg gtcggagcaa
     8101 aacatcgtcc agccgctaaa aacgctgatg gccgatgatc cggattacag tttcgatatt
     8161 ctggaagccc gctacgccat tgaagccagc accgcatggc atgcggcaat gcgcgccaca
     8221 cctggcgaca aagaaaagat tcagctttgc tttgaagcaa cgctaagtga agacccggat
     8281 atcgcctcac aagcggacgt tcgttttcat ctggcgattg ccgaagcctc acataacatc
     8341 gtgctgctgc aaaccatgcg cggtttcttc gatgtcctgc aatcctcagt gaagcatagc
     8401 cgtcagcgga tgtatctggt gccaccggtt ttttcacaac tgaccgaaca acatcaggct
     8461 gtcattgacg ccatttttgc tggtgatgcc gacggggcgc gtaaagcaat gatggcgcac
     8521 cttagttttg ttcacaccac catgaaacga ttcgatgaag atcaggctcg ccacgcacgg
     8581 attacccgcc tgcccggtga gcataatgag cattcgaggg agaaaaacgc atgattattt
     8641 ccgcagccag cgattatcgc gccgcagcgc aacgcattct gccgccgttc ctgttccact
     8701 atatggatgg tggtgcatat tctgaataca cgctgcgccg caacgtggaa gatttgtcag
     8761 aagtggcact gcgccagcgt attctgaaaa acatgtccga cttaagcctg gaaacgacgc
     8821 tgtttaatga gaaattgtcg atgccggtag cactggcacc ggtgggtttg tgtggcatgt
     8881 atgcgcgtcg tggcgaagtt caggcagcca aagcggcgga cgcgcatggc attccgttta
     8941 ctctctcgac ggtttccgtt tgcccgattg aagaagtcgc gccagccatc aagcgcccaa
     9001 tgtggttcca gctttatgta ctgcgcgatc gcggctttat gcgtaacgcg ctggagcgag
     9061 caaaagcagc gggttgttcg acgctggttt tcaccgtgga tatgccgaca ccgggcgcac
     9121 gctaccgtga tgcgcattca ggtatgagcg gcccaaatgc ggcaatgcgc cgctacttgc
     9181 aggcggtgac gcatccgcaa tgggcgtggg atgtgggcct gaacggtcgt ccacatgatt
     9241 taggtaatat ctcagcttat ctcggcaaac cgaccggact ggaagattac atcggctggc
     9301 tggggaataa cttcgatccg tccatctcat ggaaagacct tgagtggatc cgcgatttct
     9361 gggatggccc gatggtgatc aaagggatcc tcgatccgga agatgcgcgc gatgcagtac
     9421 gttttggtgc tgatggaatt gtggtttcta accacggtgg ccgccagctg gacggtgtac
     9481 tctcttccgc tcgtgcgttg cccgctattg cagatgcggt gaaaggtgat atcgccattc
     9541 tggcggatag cggaattcgt aacgggcttg atgtcgtgcg tatgattgcg ctcggtgccg
     9601 acaccgtact gctgggtcgt gctttcctgt atgcgctggc aacagcgggc caggcgggtg
     9661 tagctaacct gctaaatctg atcgaaaaag agatgaaagt ggcgatgacg ctgactggcg
     9721 cgaaatcgat cagtgaaatt acgcaagatt cgctggtgca ggggctgggt aaagagttgc
     9781 ctacggcact ggctccgatg gcgaaaggga atgcggcata gtcgtttgcg ccccctcacc
     9841 ctaaccctct ccctcaggga gaggggaccg ttcggcgctg tatgtactcc ctcactctga
     9901 aacgacaccg cactcttttt ttctccctcg cccctccggg gagggccggg gtgaggggaa
     9961 aaggccgcac ggttcaccaa ttttctgcta tcctgccccc gcactaaggg ggcagtatgc
    10021 taaacatcgt actttacgaa ccagaaattc cgccaaatac tggcaacatc atccgtcttt
    10081 gcgctaatac cggctttcgt ctacatatca tcgaaccgat gggatttgcc tgggacgaca
    10141 agcgcctgcg ccgcgcgggg ctggactatc acgagtttac cgccgttacg cgtcatcatg
    10201 actatcgcgc gttcctcgaa gcagaaaatc cccagcgcct gtttgccctc accacgaaag
    10261 gtacgcctgc tcacagcgcc gtaagctatc aggatggcga ttatctgatg ttcggcccgg
    10321 aaacacgcgg cctgccagcg agcattcttg atgccctgcc cgctgaacaa aaaattcgca
    10381 ttccgatggt gccggacagc cgcagcatga atctgtccaa tgcggtgtcg gtagtggtgt
    10441 atgaagcctg gcggcagctg ggatatccgg gagcgttgtt gagagattag ttactgtatg
    10501 ccggatagat gattacatcg catccggcac gatcacagga cattagatcc catccccata
    10561 ctcaaatgta tggttaatac cgttgaaatg ctggtccata tccattgatg gcttatcgct
    10621 gtctggttta ccgacaatac gagccggaac gccagcggcg gttgtatgcg gcggcaccgg
    10681 ttgcagcacc acggaacctg cgccaatctt cgcgccgcga ccaacttcaa tattgccgag
    10741 gattttcgcg cccgcgccaa tcatcacacc ttcacgaatt ttcgggtgac ggtcaccacc
    10801 agatttaccc gtaccgccaa gcgtcacaga ttgcagaatc gatacgtcgt tttcaattac
    10861 cgccgtttca cccacaacga tgcccgtggc gtggtcgagc ataatgccac ggccaatttt
    10921 tgctgccggg tgaatatcga cctggaacgt cacagaaacc tggttttgca gaaagattgc
    10981 caatgcgcga cgcccctgat tccacaacca gtgaccgatg cgataggcct gcaaggcatg
    11041 aaaacccttc aggtataaca acggggttga gtatttatcg actgccgggt cgcgggtacg
    11101 caccgcctga atatcacagg ccgcagaggc gatcatttcc gggtcagcgg cgtaggcttc
    11161 ttcaaccact tcacggatag caatagcagg cataattggc gatgacagct tgttcgccag
    11221 catgtagctc agtgcactgc caaggttttc gtgcttgagt agcgtcgcgt ggtaaaaact
    11281 ggccagcatt ggctcacagt ccgccagcgt tctggcttcg gctttaatat tgttccagac
    11341 aatttccagt tcttcacacg acattgctta ctccacacga tgagataatg accggcccgt
    11401 tctgcgcggg ccgggtcata gcggtaacaa aggttccctg gggttagtga ctgctgcgct
    11461 cgtccttgcg tgcacgacct aataatgtca atgctgcctc gcgcgcgttt tttccgcaat
    11521 ataatacttg ataaatttcc tcggttattg gcatttcaac gccgaagcga tgcgccagtt
    11581 cgcggacttc tttcgtattg cggtagcctt ccaccacctg accaatcttc tcctgcgcgc
    11641 tttgtacatc catgccctga ccgagcatca taccaaaacg gcggttacgc gactggttgt
    11701 cggtacaggt aagcaccaga tcgcccagcc ccgccatgcc cataaaggtg gcaggatcgg
    11761 cacccagcgc cgcaccaaga cgcgacattt cagccagccc acgggtgatc agcgccgtac
    11821 gcgcattcgc accaaaaccg ataccgtcag acatccccgc gccaatggca atgacgtttt
    11881 tcaccgcgcc gccaagctgc acgccaatga aatccgggtt gctgtaaacg cggaaacttt
    11941 tgccgcagtg cagcagctgc tggagatcat cggcaaaggt ctgatcggtc gaggccagcg
    12001 aaatagctgt cggtaaacct gccgccagtt ctttcgcaaa cgttgggcca gagataaccg
    12061 ccagcggaat ttgatcgcct aaggcctcac gcgccacgtc ctgtaacaga cgtccggttt
    12121 ccgcttccag ccctttggtc gcccacacca gacgcgcatc aggacgcatc agcggtttaa
    12181 tctggcgcag cacttcacca aagacatggc tgggtacgac gacgagaata ttacgactgg
    12241 ctgccagcgc agtggcgaga tcgctttcaa gatggagcgt atcgggaaaa ggcacatcgg
    12301 ggagaaacgc ggcgttacag cggtcgcgtt caagcgttgc gatatgttca gggtcatggc
    12361 cccagaggac aacctcgtgg ccatttcttg ccagggtaat ggcaagagcg gtgccgtacg
    12421 agccggcacc gatcacagtc attgaagcat tacgttggtt catcaggcat cctgatgttc
    12481 ttcagtacct tcgccagcct gctgctgcaa atagttcatg aacagcgcat cgaagttaac
    12541 cggcgcaagg ttcagttgcg ggaatgtacc gcgggatacc atgctggtga tgcactcacg
    12601 agcatacggg aacagaatgt tcgggcagta tgcgcccagg caatgtgcca tctgggtgcc
    12661 ttcgataccc gcgatggaga aaataccgcc ctgctgaact tcacacagga acgcggtttc
    12721 ttcgcccaag gaggccgtta cggtaacacg cagtaccact tcgtatacgt catctgccag
    12781 ttgggaagat gccgtatcca gatcaagttt aacttctggt tgccaatctt tctggaaaac
    12841 gtgcggcgcg ttcggcgctt cgaaagagat atccttggta taaatacgtt ggatctggaa
    12901 agtcatttca gtgttgtttt gttctgacat gtgtagaaaa cccttaagtg ttgtccttaa
    12961 atactgcgca atgtaatacc aacgttcaca cgttatttca gcaggggatc cagtccacca
    13021 cgtgcatcca atgcatacaa gtcatcacag ccgccaatgt gctgtgcgtc aataaaaatc
    13081 tggggaaccg tggtgcaacc gctgcgtttg atcatctctt cacgcttggc ggcattgcca
    13141 tcgatcggca actcctggaa actcacgccc ttgctgctca gcagtgcttt tgcacgatgg
    13201 caatacgggc aggtttcttt ggtatagatt tcaacattgg ccataattta gctcctgttt
    13261 ttttaccctg aaatttcatg ttgcagggcg gctgcaaaat ggagtgtccc cagaagctta
    13321 taacaataag tcgctggggg cctaattgca gctaacggcc tgcatcatga aagacgacag
    13381 gtaaattatt tgccgcgcac cagaggcaag ttttcgccag cccagccagc aacgccctct
    13441 ttcagcacga atacttgcgc aaaaccagct ttcgtcagcg cgtttgcagg ctcctggcac
    13501 tgcatgccag aaccgtctac cacgataacc gatttgtctt tgtgcttctc aagctcacca
    13561 acattgttgg ctttgatttc gctcggcaac agattgatag aacctgcgat atggccttta
    13621 cggaagtcat cacgctgacg taaatccaca accacagcgt cttctttgtt gatcagacgc
    13681 gtagcttcac cacgagtaat caccttcact ttcgaggtca ggctcttaaa cgtagtcaca
    13741 agaaccgcca ccagtaacgc gatccaggcg atactcagta tgggatgacg gccaacaaat
    13801 tgcataattt cttgcatggg gggtaacaac tcccgacgta gtgattaaaa aaaccaggaa
    13861 aggagtatac ctgcgcggtg cggcaaatac agccagcgcg ttaactggaa tgcaattttg
    13921 cggggcacga cgaaaaaaaa gcgcaaatct ggcatcggct tgccgcgagc ggtcgtatat
    13981 tttgatcttc agaggctatt ttatcgattc agctgtagta aaattacgca aattttgact
    14041 cttgagtatg aggttgtcgc aatgtcggtt tctaaaaaac ctatggtact ggtgattctg
    14101 gatggctatg gctatcgtga agaacagcag gataacgcca tttttagtgc taaaaccccg
    14161 gtaatggatg cactgtgggc caatcgtccg cataccctga tcgacgcttc cggtctggaa
    14221 gtcggtctgc ctgaccgtca gatgggtaac tccgaagtag gccacgttaa cctgggtgcc
    14281 ggccgcatcg tgtatcagga cctgactcgt ctggacgttg aaatcaaaga tcgcgctttc
    14341 tttgctaatc cagtgctggc tggcgcggta cataaagcga aaaacgcagg taaagcagta
    14401 cacattatgg gtctgctctc cgcaggcggc gtacacagcc acgaagatca catcatggcg
    14461 atggtagaac tggcagctga acgcggcgca gaaaaaatct acctgcacgc attccttgac
    14521 ggtcgcgaca ccccgccgcg cagtgctgaa tcctcgctga aaaaatttga agagaaattt
    14581 gctgcgctgg gtaaaggccg cgtagcgtcc atcattggtc gttactacgc gatggaccgc
    14641 gataaccgtt gggatcgcgt tgaaaaagcc tatgacctgc tgactctggc gcagggtgag
    14701 ttccaggccg atactgccgt tgctggtttg caggcagctt atgctcgcga cgaaaacgac
    14761 gagttcgtga aagcgaccgt tattcgtgca gaaggtcagc cagatgcggc aatggaagac
    14821 ggcgatgcgc tgattttcat gaacttccgt gctgaccgcg cgcgtgaaat cactcgtgca
    14881 ttcgtgaacg ctgatttcga tggcttcgcg cgtaagaaag tggttaacgt cgatttcgtg
    14941 atgctgaccg aatacgccgc tgacatcaaa actgcggttg cttacccacc cgcttccctg
    15001 gttaacacct tcggcgagtg gatggcgaaa aacgacaaaa ctcagttgcg tatttccgaa
    15061 accgaaaaat atgcccacgt tactttcttc ttcaacggtg gcgtagaaga gtcgttcaaa
    15121 ggcgaagatc gcattctgat caactcaccg aaagtggcta cctacgatct gcaaccggaa
    15181 atgagctccg cagagctgac cgaaaaactg gttgcggcca tcaagagcgg caaatacgac
    15241 accattatct gtaactatcc gaacggcgac atggtaggtc acaccggggt gatggaagcg
    15301 gcggttaaag cggttgaagc actggatcac tgcgtggaag aagtcgcgaa agcggttgag
    15361 tccgtgggtg gacaactgct gatcaccgct gaccacggta acgctgaaca gatgcgcgat
    15421 ccggcaacgg gtcaggcaca cacggcacac accaacctgc cagttccgct gatttacgtt
    15481 ggtgataaga acgtgaaagc ggttgcaggc ggcaaacttt ctgacatcgc gccgaccatg
    15541 ttgtcgctga tgggtatgga aatcccgcaa gagatgactg gtaagccgct gttcatcgtg
    15601 gaataatccc tccccatgag gggaaaggcg attaatacca tgacacgggc cgtgaaaccg
    15661 cgcaggtttg caatcaggcc catcatctac gccagcgttc ttagcgctgg cgtattgttg
    15721 tgcgcctttt ccgcccacgc ggatgagcgt gaccaactca aatctattca ggccgatatc
    15781 gccgcaaaag agcgcgcggt acgccaaaag caacaacaac gcgcaagcct gctcgcacaa
    15841 ttgaaaaagc aggaagaagc gatctctgaa gccacccgta agctgcgcga aacgcaaaac
    15901 acgctcaatc aactcaataa acagattgat gagatgaacg cgtcgattgc caaactggag
    15961 cagcaaaaag ccgcccagga gcgcagcctc gccgcacaac tggatgccgc attccgtcag
    16021 ggcgagcata ccggtattca gctaattctc agcggtgaag aaagccagcg tggacagcgt
    16081 ttacaggctt atttcggcta tctcaaccag gcgcgacaag aaaccattgc ccagttgaag
    16141 caaacgcgtg aagaagtcac catgcagcat gctgaactgg aagagaaaca aagcgagcaa
    16201 caaacgctgt tatatgaaca gcgcgcccaa caggcgaaac tgactcaggc tctgaacgag
    16261 cgtaaaaaga cgctggcggg gctggaatct tccatccagc aaggtcagca gcagctaagt
    16321 gaactgcgtg ccaacgaatc ccgtctgcgt aacagcattg cccgtgctga agccgcggcg
    16381 aaagcgcgtg ctgaacgaga agcgcgtgag gcccaggcgg ttcgcgagcg ccagaaagaa
    16441 gcgacgcgca aaggcaccac ctacaaaccg accgaaagcg aaaaatcgct gatgtcccga
    16501 actggtggcc tgggtgcgcc gcgtggtcag gcattctggc cggttcgcgg gccgacgctg
    16561 catcgctatg gcgagcagct acagggtgaa ttacgctgga aagggatggt tatcggtgcc
    16621 tctgaaggta ctgaagttaa agcaattgcc gacggtcgag tgattctggc tgactggctg
    16681 caaggttacg gtctggtggt ggtggttgag cacggtaaag gcgacatgag tctttacggc
    16741 tataatcaga gcgcactggt gagcgttggt tcgcaggttc gcgcgggcca gccaattgca
    16801 ctggtgggca gcagtggcgg tcagggtcgg ccttcactct atttcgaaat tcgccgccag
    16861 ggtcaggcgg tcaatccaca gccgtggttg ggaagataag ttttgtttcc atttcgtcgt
    16921 aacgttcttg catttgccgc tctgttggcg ctctcctccc ccgtacttgc tggcaaactt
    16981 gccatcgtca ttgatgattt tgggtatcgc ccgcacaacg aaaaccaggt gctggcgatg
    17041 ccttccgcta tctccgtcgc tgtattaccc gattcaccgc acgccagaga gatggcgacc
    17101 aaagcgcata acagcggtca cgaagtgttg attcatctcc cgatggcacc gttgagtaaa
    17161 cagccgctgg agaaaaatac gctacgcccg gagatgagca gcgacgaaat tgagcgcatt
    17221 attcgtagtg cggtcaataa cgtgccctat gccgtgggga tcaacaacca catgggcagc
    17281 aagatgacct ctaacctgtt tggtatgcag aaagtgatgc aggcgctgga gcgttacaat
    17341 ctttacttcc tcgacagcgt aaccatcggt aatacccagg cgatgcgcgc cgcgcaaggt
    17401 actggcgtga aggtgatcaa acggaaggtg ttcctcgacg attcgcagaa cgaagcggac
    17461 atccgtgtgc aatttaatcg cgcaattgat ctggcgcgtc gcaacggttc aaccattgcc
    17521 attgggcatc ctcacccttc aacggtacgc gtgctacaac agatggttta taacctgccg
    17581 ccagacatta cgctggtgaa agccagcagc ttgctgaatg aaccgcaggt tgatacttct
    17641 acaccgccga aaaacgctgt gcctgatgca ccgcgtaatc cgttccgtgg cgtgaagctg
    17701 tgcaaaccga agaaaccgat agaacctgtt tatgctaatc gcttctttga agtattaagc
    17761 gaaagcatca gccagagcac gctgatcgtt tacttccagc atcagtggca aggctggggc
    17821 aaacagcccg aagcggcgaa gtttaacgct agcgcaaatt aaaggcgcta cgcgtaatgc
    17881 gatggtgcga ccgcgttttg tcgcgccatt gccataagcg gaaagaccac agtaacgcct
    17941 ggtaaccgac cttcacgctg cgcacattgg taatcaggcg cttatacata cccggaagta
    18001 aatatctcgg caatcatccg ttggcgggta agaatatccg gctctttgcg c
//
LOCUS       DAJTRC010000079        17132 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.079, whole genome shotgun sequence.
ACCESSION   DAJTRC010000079 DAJTRC010000000
VERSION     DAJTRC010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17132)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17132)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17132
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.079"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..203)
                     /locus_tag="OO357_002896"
     CDS             complement(<1..203)
                     /locus_tag="OO357_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="represses the celABCDF-ydjC operon involved in
                     carbon uptake; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator ChbR"
                     /protein_id="HCR5887773.1"
                     /translation="MMQPVINAPEIATAREQQLFNGKNFHVFIYNKTESISGLHQHDY
                     YEFTLVLTGRYFQEINGKRVLLER"
     gene            complement(211..561)
                     /gene="chbA"
                     /locus_tag="OO357_002897"
     CDS             complement(211..561)
                     /gene="chbA"
                     /locus_tag="OO357_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIA"
                     /protein_id="HCR5887774.1"
                     /translation="MMDLDNIPDTQTEAEELEEVVMGLIINSGQARSLAYAALKQAKQ
                     GDFAAAKAMMDQSRMALNEAHLVQTKLIEGDAGEGKMKVSLVLVHAQDHLMTSMLARE
                     LITELIELHEKLKA"
     gene            complement(620..1968)
                     /gene="chbC"
                     /locus_tag="OO357_002898"
                     /pseudo
     CDS             complement(620..1968)
                     /gene="chbC"
                     /locus_tag="OO357_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707374.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIC"
     gene            complement(2053..2373)
                     /gene="chbB"
                     /locus_tag="OO357_002899"
     CDS             complement(2053..2373)
                     /gene="chbB"
                     /locus_tag="OO357_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N,N'-diacetylchitobiose transporter subunit
                     IIB"
                     /protein_id="HCR5887775.1"
                     /translation="MEKKHIYLFCSAGMSISLLVSKMRAQAEKYEVPVIIEAFPETLA
                     GEKGQNADVVLLGPQIAYMLPEIQRLLPNKPVEVIDSLLYGKVDGLGVLKAAVAAIKK
                     AAAN"
     gene            complement(2672..3010)
                     /gene="osmE"
                     /locus_tag="OO357_002900"
     CDS             complement(2672..3010)
                     /gene="osmE"
                     /locus_tag="OO357_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotically-inducible lipoprotein OsmE"
                     /protein_id="HCR5887776.1"
                     /translation="MNKNMAGILSAAAVLTMLAGCTAYDRTKDQFVQPVVKDVKKGMS
                     RAQVAQIAGKPSSEVSMIHARGTCQTYILGQRDGKAETYFVALDDTGHVINSGYQTCA
                     EYDTDPQAAK"
     gene            3212..4039
                     /gene="nadE"
                     /locus_tag="OO357_002901"
     CDS             3212..4039
                     /gene="nadE"
                     /locus_tag="OO357_002901"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HCR5887777.1"
                     /translation="MTLQQQIIKALGAKPQINAEEEIRRSVDFLKSYLQTYPFIKSLV
                     LGISGGQDSTLAGKLCQMAINELRQETRNESLQFIAVRLPYGVQADEQDCQDAIAFIQ
                     PDRVLTVNIKGAVLASEQALREAGIELSDFVRGNEKARERMKAQYSIAGMTSGVVVGT
                     DHAAEAITGFFTKYGDGGTDINPLYRLNKRQGKQLLTALGCPEHLYKKAPTADLEDDR
                     PSLPDEVALGVTYDNIDDYLEGKNLPEQVARTIENWYLKTEHKRRPPINVFDDFWKK"
     gene            4269..5156
                     /gene="cho"
                     /locus_tag="OO357_002902"
     CDS             4269..5156
                     /gene="cho"
                     /locus_tag="OO357_002902"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707370.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease Cho"
                     /protein_id="HCR5887778.1"
                     /translation="MVRRLTSPRLEFEAAAIYEYPEHLHSFLNDLPTRPGVYLFHGES
                     DTMPLYIGKSVNIRSRVLSHLRTPDEAAMLRQSRRISWICTAGEIGALLLEARLIKEQ
                     QPLFNKRLRRNRQLCALQLNEKRVDVVYAKEVDFSRAPNLFGLFANRRAALQALQSIA
                     DEQKLCYGLLGLEPLSRGRACFRSALKRCAGACCGKESHEEHALRLRQSLERLRVVCW
                     PWQGAVALKEQHPEMTQYHIIQNWLWLGAVNSLEEATTLIRTPAGFDHDGYKILCKPL
                     LSGNYEITELDPANDQRAS"
     gene            complement(5116..5691)
                     /gene="ves"
                     /locus_tag="OO357_002903"
     CDS             complement(5116..5691)
                     /gene="ves"
                     /locus_tag="OO357_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707369.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="environmental stress-induced protein Ves"
                     /protein_id="HCR5887779.1"
                     /translation="MEYFDMRKMSVNLWRNAAGETREICTFPPAKRDFYWRASIASIA
                     ANGEFSLFPGMERIVTLLEGGEMLLESADRFNHTLKPLQPFAFTADQVVKAKLTAGQM
                     SMDFNIMTRLDVCKAKVRIAERTFTTFGSRGGVVFVINGAWQLGDKLLTTDQGVCWFD
                     GRHTLRLLQPQGKLLFSEINWLAGHSPDQVQ"
     gene            complement(5894..6379)
                     /gene="spy"
                     /locus_tag="OO357_002904"
     CDS             complement(5894..6379)
                     /gene="spy"
                     /locus_tag="OO357_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-independent periplasmic protein-refolding
                     chaperone"
                     /protein_id="HCR5887780.1"
                     /translation="MRKLTALFVASTLALGAANLAHAADTTTAAPADAKPMMHHKGKF
                     GPHQDMMFKDLNLTDAQKQQIHEIMKGQRDQMKRPPLEERRAMHDIIASDTFDKAKAE
                     AQIAKMEEQRKANMLAHMETQNKIYNILTPEQKKQFNANFEKRLTERPAAKGKMPATA
                     E"
     gene            complement(6709..7677)
                     /gene="astE"
                     /locus_tag="OO357_002905"
     CDS             complement(6709..7677)
                     /gene="astE"
                     /locus_tag="OO357_002905"
                     /EC_number="3.5.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase"
                     /protein_id="HCR5887781.1"
                     /translation="MDNFLALTLTGKKPVITEREINGVRWRWLGDGVLELTPLTPPQG
                     ALVISAGIHGNETAPVEMLDALLGAISHGEIPLRWRLLVILGNPPALKQGKRYCHSDM
                     NRMFGGRWQLFAESGETCRARELEQCLEDFYDQGKESVRWHLDLHTAIRGSLHPQFGV
                     LPQRDIPWDEKFLTWLGAAGLEALVFHQEPGGTFTHFSVRHFGALACTLELGKALPFG
                     QNDLRQFAVTASAIAALLSGESVGIVRTPPLRYRVVSQITRHSPSFEMHMASDTLNFM
                     PFEKGTLLAQDGEERFTVTHDVEYVLFPNPLVALGLRAGLMLEKIS"
     gene            complement(7670..9003)
                     /gene="astB"
                     /locus_tag="OO357_002906"
                     /pseudo
     CDS             complement(7670..9003)
                     /gene="astB"
                     /locus_tag="OO357_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416259.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="N-succinylarginine dihydrolase"
     gene            complement(9000..10478)
                     /gene="astD"
                     /locus_tag="OO357_002907"
     CDS             complement(9000..10478)
                     /gene="astD"
                     /locus_tag="OO357_002907"
                     /EC_number="1.2.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate-semialdehyde dehydrogenase"
                     /protein_id="HCR5887782.1"
                     /translation="MTLWINGDWITGQGASRVKRNPVSGEVLWQGNDADAAQVEQACR
                     AARAAFPRWARLSLAERQVVVERFAGLLESNKAELTAIIARETGKPRWEAATEVTAMI
                     NKIAISIKAYHVRTGEQRSEMPDGAASLRHRPHGVLAVFGPYNFPGHLPNGHIVPALL
                     AGNTIIFKPSELTPWSGEAVMRLWQQAGLPPGVLNLVQGGRETGQALSALEDLDGLLF
                     TGSANTGYQLHRQLSGQPEKILALEMGGNNPLIIDEVADIDAAVHLTIQSAFVTAGQR
                     CTCARRLLLKSGAQGDAFLASLVAVSQRLTPGNWDDEPQPFIGGLISEQAAQQVVTAW
                     QQLEAMGGRTLLAPRLLQAGTSLLTPGIIEMTGVGGVPDEEVFGPFLRVWRYDTFDEA
                     IRMANNTRFGLFCGLVSPEREKFDQLLLEARAGIVNWNKPLTGAASTAPFGGIGASGN
                     HRPSAWYAADYCAWPMASLESDSLTLPATLNPGLDFSDEVVR"
     gene            complement(10475..11509)
                     /gene="astA"
                     /locus_tag="OO357_002908"
     CDS             complement(10475..11509)
                     /gene="astA"
                     /locus_tag="OO357_002908"
                     /EC_number="2.3.1.109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine N-succinyltransferase"
                     /protein_id="HCR5887783.1"
                     /translation="MMVIRPVERSDVSALMQLASKTGGGLTSLPANEATLSARIERAI
                     KTWQGELPKSEQGYVFVLEDSETGTVAGICAIEVAVGLNDPWYNYRVGTLVHASKELN
                     VYNALPTLFLSNDHTGSSELCTLFLDPDWRKEGNGYLLSKSRFMFMAAFRDKFNDKVV
                     AEMRGVIDEHGYSPFWQSLGKRFFSMDFSRADFLCGTGQKAFIAELMPKHPIYTHFLS
                     QEAQDVIGQVHPQTAPARAVLEKEGFRYRNYIDIFDGGPTLECDIDRVRAIRKSRLVE
                     VAEGQPAQGDFPACLVANENYHHFRVVLARTDPATERLILTAAQLDALKCHAGDRVRL
                     VRLCAEEKTA"
     gene            complement(11506..12726)
                     /gene="astC"
                     /locus_tag="OO357_002909"
                     /pseudo
     CDS             complement(11506..12726)
                     /gene="astC"
                     /locus_tag="OO357_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416262.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="succinylornithine/acetylornithine transaminase"
     gene            13172..13978
                     /gene="xthA"
                     /locus_tag="OO357_002910"
     CDS             13172..13978
                     /gene="xthA"
                     /locus_tag="OO357_002910"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HCR5887784.1"
                     /translation="MKFVSFNINGLRARPHQLEAIVEKHQPDVIGLQETKVHDDMFPL
                     EEVAKLGYNVFYHGQKGHYGVALLTKETPIAVRRGFPGDDEEAQRRIIMAEIPSLLGN
                     VTVINGYFPQGESRDHPIKFPAKAQFYQNLQNYLETELKRDNPVLIMGDMNISPTDLD
                     IGIGEENRKRWLRTGKCSFLPEEREWMDRLMSWGVVDTFRHANPQTADRFSWFDYRSK
                     GFDDNRGLRIDLLLASQPLAECCVETGIDYEIRSMEKPSDHAPVWATFRR"
     gene            14145..14858
                     /locus_tag="OO357_002911"
     CDS             14145..14858
                     /locus_tag="OO357_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707363.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5887785.1"
                     /translation="MNAERNFLFACLIFALVIYAIHAFGLFDLLTDLPHLQTLIRQSG
                     LFGYSLYILLFINAPLFLLPGSILVIAGGIVFGPLLGTQLSLIAATLASSCSFLLARW
                     LGRDLLLKYVGHSHTFQAIEKGIARNGIDFLILTRLIPLFPYNIQNYAYGFTTIAFWP
                     YTLISALTTLPGIVIYTVMASDLANEGITLRFILQLCLAGLALFILIQLAKLLYARHK
                     HVDLSASRRSPLTHPKNEG"
     gene            14863..15540
                     /gene="ydjY"
                     /locus_tag="OO357_002912"
     CDS             14863..15540
                     /gene="ydjY"
                     /locus_tag="OO357_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YdjY"
                     /protein_id="HCR5887786.1"
                     /translation="MSQHYSVSWKKGLAALCLLAVAGLSGCDQKENAAAKVEYDGLSN
                     SQPLRVDANNHTVTMLVQINGRFLTDDTRHGIVFKDGSNGHKSLFMAYAPPKAFYEAL
                     KEAGGTPGENMTMDNKETTHVTGSKLDISVNWQGAAKAYSFDEVIVDSNGKKLDMRFG
                     GNLTAAEEKKTGCLVCLDSCPVGIVSNATYTYGAVEKHGEVKFKGNASVLPADNTLAT
                     VTFKITE"
     gene            15555..16262
                     /locus_tag="OO357_002913"
     CDS             15555..16262
                     /locus_tag="OO357_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001697152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HCR5887787.1"
                     /translation="MMKIQSRKIWYYRITLIILLFAMLLAWALLPGVHEFINRSVAAF
                     AAVDQQGIERFIQSYGALAAVVSFLLMILQAIAAPLPAFLITFANASLFGAFWGGLLS
                     WTSSMAGAALCFFIARVMGREVVEKLTGKTVLDSMDGFFTRYGKHTILVCRLLPFVPF
                     DPISYAAGLTSIRFRSFFIATGLGQLPATIVYSWAGSMLTGGTFWFVTELFILFALTV
                     VIFMAKKIWLERQKRND"
     gene            16262..16810
                     /locus_tag="OO357_002914"
     CDS             16262..16810
                     /locus_tag="OO357_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HCR5887788.1"
                     /translation="MGLPPLSKIPFILRPQAWLHRRHYGEVLSPIRWWGRIPFIFYLV
                     SMFVGWLERKRSPLDPVVRSLVSARIAQMCLCEFCVDITSMKVAERTGSTDKLLAVAD
                     WRQSPLFSDEERLALEYAEAASVTPPTVDDALRTRLAAHFDAQALTELTALIGLQNLS
                     ARFNSAMDIPAQGLCRIPEKRS"
     gene            16820..>17132
                     /locus_tag="OO357_002915"
     CDS             16820..>17132
                     /locus_tag="OO357_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HCR5887789.1"
                     /translation="MRHCGWLLGLLSLFSLATHASDWQEIKNEAKGQTVWFNAWGGDT
                     AINRYLDWVSGEMKTHYAINLKIVRLADAADAVKRIQTEAAAGRKTGGSVDLLWVNGE
                     NF"
BASE COUNT         4205 a         4564 c         4216 g         4147 t
ORIGIN      
        1 cgttccagta acacgcgctt accgttaatc tcctggaaat aacgcccggt taataccaga
       61 gtaaattcat aatagtcgtg ctgatgcagt ccgctgatac tctcagtttt gttatagata
      121 aacacatgga agtttttgcc attaaacaac tgctgttctc gggcagtggc aatttccggc
      181 gcgttaatca ctggctgcat catcgactcc ttatgccttc agtttttcat gaagctcaat
      241 taattcagta atcagttcac gcgcaagcat ggacgtcatt aaatgatcct gagcgtggac
      301 cagcaccaga ctcactttca tcttaccttc gcccgcatcg ccttcaatca gtttcgtctg
      361 taccagatgc gcttcattca atgccatccg tgactgatcc atcatggctt tcgctgcggc
      421 aaaatcgccc tgcttcgcct gtttcaatgc ggcatacgcc aggctacgcg cttgtccaga
      481 gttgatgata agccccatca ccacttcttc cagctcttca gcttctgttt gcgtatcagg
      541 aatattatcg agatccatca tacatcgttc ctctctcttc ttaccggcaa gattacccgt
      601 accggcatcg attaaaattt caggggttag cgatatcttc ttcgctctct tctttatcaa
      661 tcgcattttg tgctttgtta gccaccacaa caaagggcag ataaattaac gttgcgatgc
      721 caaggttgaa gagtgcgacc agcaatgcgg cgacgctacc gttggtgtta aagaaggctc
      781 ccagaccggt tggcatggtc cacggtgcaa tattggtcac cggaggaatg atgcccatgt
      841 agtacgctgc gagggtgatt gccgccagaa tcggttgtac cagtacaaac gggataaaca
      901 tcaccgggtt catgataatt ggcagaccaa acagaatcgg ttcgttaatc tggaagatgc
      961 cggacggcag cgccagtttt gccacctgac gataatcagc acggcgagag gcgataaaga
     1021 tagccaggat caggcctaaa gtcgcaccac tgcccccaag gaaaataaag gagtccagca
     1081 tcggcttggc ccagatatgg aaggtcttac cggctgccag cgccgcttcg acggaaccat
     1141 attgctgata ggtcgcgata ttttccagcg cccacggcgt cataatgccg ttgtccagtg
     1201 cggtcagcgc cagcgcgcca tgaataccga agaaccagag cagtggaaca aagatcacat
     1261 aggcccagcc caccacgcta cccaacgatg ccagtggggt tgagatggta tccataatga
     1321 tctgatggaa gttggtgccc caggtattca acgcccaggc aataatcccc atcacggaaa
     1381 gaataataaa gccgggaatt aatgccgaga aagaacgcga tactgaagca ggtacgctgt
     1441 cgggcagttt aatgacccaa ttgcggcgga caataaaggt aaacatttct gccaccacca
     1501 ggccaataat aatcccggag atgatattcg ccccacctaa ccagtttgcg ccaaccgcat
     1561 aggcctcacc gacactatat ggggtgacgg tcataaatgc tgcaacggat aacaacccag
     1621 ccgccagcgc atcgacttta cgctcttctg ccagcgccat gccaataaag aacggtgcca
     1681 ttaaagacat tattcctaat gttccgttat atacgttgcc gccaatacct ttcagaccat
     1741 taagtgtttc aatggttgag gcgtcgaggc gaatacctaa ggaataaaaa acgacccctc
     1801 cccaaagctt agaaaaacgt tgttaattaa tacaaacatg gcccccgcaa gggttaacgg
     1861 cattaagcga ataaagccat ttttgattgc attaacgtgt ggctgctttc ctattttaac
     1921 tgcaaaaggg aggagtacct tttcaagcga tgcaataaca ttactcatag aaaaataccc
     1981 ttaaaaaccg caatttaaat attacggtat tgatttatga aataactctt tgacgggaaa
     2041 atttaaaata aattaatttg ctgcggcttt tttaatcgct gcaaccgcag ccttaagcac
     2101 gcctaaacca tcgactttgc cataaagcag cgagtcaatt acttcaaccg gtttgttggg
     2161 taacaaacgc tggatttcgg gcaacatata agcaatctgc ggccctaata acacgacatc
     2221 ggcattctga cctttttcac cagccagtgt ttccggaaat gcttcaataa tgaccggaac
     2281 ttcatatttt tctgcctgtg cgcgcatttt tgataccagt aaagagatag acatgcccgc
     2341 agaacaaaac agataaatgt gtttcttttc cataaaactg ccctcatcga cgattatctg
     2401 tcagccagac actccgcaag ccttgacctg cttccatgct ctgggtaact tgcgaaacca
     2461 acatgacttt ttttgtggct gaaaggagta tacggtatag acctgagcaa tagtatttct
     2521 ttgaccttat aaattgtttc tccttgacct gtggttatga ccctcttcac atttcgggca
     2581 aataattcgc gaagataatt aagaactcag gccaaaaaaa accggcgcaa tggccggttt
     2641 ccgttgttac tcaagctttc agacgaattg attacttcgc agcctgtgga tcagtgtcgt
     2701 attcagcaca ggtctgataa ccggagttga tgacatgtcc ggtatcatct aacgcgacaa
     2761 agtaggtttc tgctttacca tcacgttgac ccaggatgta ggtctggcaa gtaccgcgag
     2821 catggatcat gctcacttca gacgaaggtt tacccgcaat ttgtgcaacc tgcgcccggc
     2881 tcatgccttt tttgacgtct ttcaccacag gctgtacaaa ctggtctttg gtacgatcat
     2941 aagctgtaca acccgccagc atggttaata ccgccgctgc actcagaatt cctgccatat
     3001 tcttgttcat attccgtcct cttgtttatc agcgtgttag ataagcctgg aatacattgg
     3061 gcgctttttc aagcccgtga acgaaacggc tccgctttca gaggattcct gtatgacgtt
     3121 ttaaccacca ttcagcccgc tgtcgcttgt cgtttcagta gcaacgggtt agctttaagg
     3181 aagttttgtc ttttctgtct ggaggggttc aatgacattg caacaacaaa taataaaggc
     3241 gctgggcgca aaaccgcaga ttaatgctga agaggaaatt cgtcgtagtg tcgattttct
     3301 gaaaagctac ctgcaaactt atccgttcat taaatcactg gtgctcggga tcagcggcgg
     3361 tcaggactcc acgcttgccg gaaagctgtg ccagatggcg attaatgagc tgcgccagga
     3421 aactcgcaac gaatcactgc aatttattgc cgtacgcctg ccctatggtg ttcaggctga
     3481 cgaacaagat tgccaggatg ccattgcctt tattcaaccg gatcgcgtat taaccgttaa
     3541 tatcaagggc gcggtattgg ctagcgagca ggcattgcgg gaagcaggca ttgaactgag
     3601 cgattttgtc cgtggcaatg aaaaagcgcg tgagcggatg aaagcacaat atagcattgc
     3661 gggtatgacc agcggtgtcg tggtgggcac cgatcatgca gcagaagcca ttaccggatt
     3721 cttcactaaa tatggtgacg gcggtacgga tattaatccg ctgtatcgtc tcaacaaacg
     3781 ccagggtaaa cagttactga cggcattagg ttgcccggaa cacctttata agaaagcccc
     3841 aactgccgat cttgaagatg atcgcccttc tctgccagat gaagtggcac tcggcgtgac
     3901 ctatgacaat atcgacgact atctggaagg gaaaaaccta cctgaacagg tcgccagaac
     3961 aatagagaac tggtatctga aaaccgaaca taaacgccgt ccgccaatta acgttttcga
     4021 tgatttctgg aaaaagtaat aatttgcacc tattggattg tgcgaaaaag agtaatttgt
     4081 tcacgccgga tgcggcgtga acgccttatt ctacctacaa aactatgcaa attcaataca
     4141 ttgcaggagt cgaataggcc tgacaggcgt agcacgtcag acggtgtaac ctttgtcatc
     4201 aacccgcctc ttttttaatc gcctcccgcc tgttacactg gatagataac cagcattcgg
     4261 agtcaacagt ggtacggcgt ttaacttctc cgcggctcga atttgaagct gcggcaattt
     4321 atgaatatcc cgaacattta cattcattcc ttaatgactt acccacccga ccaggggtgt
     4381 atctgtttca tggcgaaagt gacaccatgc cgctctatat cggcaaaagc gttaatatcc
     4441 gcagtcgggt gctttctcat ttacgcaccc cggatgaagc cgccatgcta cggcaatccc
     4501 ggcgaattag ctggatctgc accgcaggcg aaatcggcgc tctgctcctt gaagcgcgat
     4561 taatcaaaga acaacagccg ctgtttaata aacggttgcg ccgcaatcgc cagctctgtg
     4621 ccctgcaatt aaatgaaaag cgcgtcgatg tggtgtatgc caaagaggtg gatttttcac
     4681 gagcccccaa cctgtttggc ctgtttgcca ataggcgcgc agctttgcaa gcattgcaga
     4741 gcatcgctga tgaacaaaaa ctttgttatg gcctgttggg actggaaccg ttaagtcgcg
     4801 gtcgtgcatg ttttcgttca gcgctaaaac gttgcgccgg agcatgctgc ggtaaagaga
     4861 gccatgagga acatgcgcta cgcttgcgcc agtctctgga gcgtttgcga gtggtgtgtt
     4921 ggccttggca aggtgcggtg gcactgaaag aacagcaccc ggaaatgact caatatcata
     4981 ttattcaaaa ctggctgtgg ctgggggcgg ttaattcgct ggaagaagcg acaacgttaa
     5041 ttcggacacc cgccgggttt gatcacgacg gttataaaat tctttgtaag ccgctgcttt
     5101 ccggtaacta tgaaattact gaacttgatc cggcgaatga ccagcgagcc agttgatttc
     5161 actgaacaac agtttacctt gcggttgtaa aagccgcagg gtgtgcctgc catcgaacca
     5221 gcacacgccc tggtcggtag tgagcagttt gtcgcccagt tgccacgccc cgttaatcac
     5281 aaacaccacc ccgccgcgtg agccaaaggt agtaaacgta cgttcggcaa ttctgacttt
     5341 cgccttacag acatccaggc gcgtcataat attgaaatcc atcgacatct gccctgccgt
     5401 cagtttcgct tttaccacct ggtccgccgt aaaggcaaaa ggctgtaacg gttttaaggt
     5461 atggttaaag cggtctgcgc tttcaaggag catctcgccg ccttccagca acgtcactat
     5521 cctttccatg ccgggaaaca aagaaaactc cccattcgcc gcgatggatg caatgctggc
     5581 acgccagtaa aaatcacgtt ttgccggtgg gaaagtgcaa atttcgcgcg tttcaccggc
     5641 agcgtttcgc cacagattca ccgacatttt acgcatatca aagtattcca tactcgctcc
     5701 cggcccgttc ttttcattgt tatcgttcag gtaaccgtgg atggttatcg gcaactcggg
     5761 tgatttacgt tagtggtgat caggcttttt ttacaactgt ttgagaagag aaaagaaaac
     5821 cgccgatcct gtccaccgca ttactgcaag gtagtggaca agaccggcgg tcttaagttt
     5881 tttggctgaa agattattca gcagttgcag gcattttacc ttttgccgct ggacgttctg
     5941 tcagacgctt ctcaaaatta gcattaaatt gctttttctg ttccggcgtc aggatgttgt
     6001 aaattttgtt ctgggtttcc atgtgcgcca gcatgttagc tttgcgctgt tcttccattt
     6061 ttgcgatctg cgcttcagct tttgctttat cgaaggtatc gctggcaatg atgtcatgca
     6121 ttgcgcggcg ttcttccagc ggcggacgtt tcatctggtc acgctggcct ttcatgattt
     6181 cgtggatctg ctgtttctgc gcgtcggtca ggttcaggtc tttgaacatc atgtcctgat
     6241 gcggaccgaa cttgcctttg tggtgcatca tcggcttcgc gtcagccggt gctgcggtag
     6301 tggtgtctgc ggcatgggcc aggttagccg cgccaagagc cagggtagag gcaacaaaca
     6361 gtgcagttaa tttacgcata ttctatatcc ttcctttcag ttatttatta cggctttctt
     6421 aagtagcgtg ccgtgttgac gagattaact ttactgactt tagcgtcaat taatcagagc
     6481 aacggtaaaa caatgaaagt gtaaaaaaca cttttgcgcc aattatggag aaaaaaagaa
     6541 aatttgatgg agagtgatga gagaatatta caacacgatg attttgcaga gattatgaag
     6601 aactataccg gatgactggt gataaataaa gcaaataacc aggattaatc tgtattaatt
     6661 tataagaaag caacttaata cccgcagaat gatttctgcg ggtaagtatt agcttatttt
     6721 ttcgagcatt aatcccgcgc gtaatcccaa cgctaccaac ggattaggga ataacacata
     6781 ctctacatca tgggttacgg taaaacgttc ctctccgtcc tgcgccagca atgttccttt
     6841 ctcaaacggc ataaaattca gcgtgtcact tgccatatgc atttcgaagg acggcgagtg
     6901 gcgagtaatt tgcgaaacca cccgataacg gagcggcggt gttctcacga taccgacact
     6961 ctcaccagat agcagcgcag caattgcgct ggcagttact gcaaactggc gaagatcgtt
     7021 ttgcccaaag ggcaacgctt tgccaagttc caacgtacag gccagcgcgc caaaatgtct
     7081 gacgctgaaa tgggtaaacg taccaccagg ctcctgatgg aacaccagcg cctccagccc
     7141 cgccgcaccc agccacgtca gaaatttctc gtcccaggga atgtcgcgtt gcggtaatac
     7201 accgaactgc ggatgcaagg agccacgaat tgcggtatgt agatcaaggt gccaacgcac
     7261 agattctttg ccctggtcat aaaaatcttc caggcactgt tccagttcgc gcgcccgaca
     7321 ggtttctccg ctttcagcaa atagctgcca acgaccgcca aacattcggt tcatatcgct
     7381 atggcaataa cgtttccctt gcttcagcgc aggaggattc ccgaggatca ccagcaaccg
     7441 ccaacgtaac gggatctcgc cgtgagatat cgcgccaagc aacgcgtcca gcatttccac
     7501 aggtgccgtc tcattaccgt ggatccccgc agaaatcacc agcgcgcctt gcggtggcgt
     7561 taatggcgtc agttccagca caccatcgcc cagccagcgc cagcgaacgc cgttgatttc
     7621 tcgctcggtg ataaccggtt ttttacccgt taaggtcaga gcaagaaaat tatccattgc
     7681 cccctccctc gcgctggaac ggataaaccg aaccgagatt cagtaattgg ctcaatacat
     7741 ccagtgcttc ccgcccttcg cgcagcaatt gcgggtcggc cagatcggca gcagtaagac
     7801 gatcgcggta gtaacgatcc acccagtcat tgagcgcatt aaacaaggta tcgttcatca
     7861 tcaccgccgg attcaccgcc cggcgttctt cttctgtcaa taccacccgc aaccgcaggc
     7921 acgccgggcc gccgccattc gccatgcttt cacgtaagtc aaagactttt agttcgctaa
     7981 tcgggttgtc agcggcaagg agttcattga gataacccca tactccggcg tgttcccgac
     8041 actcctgagg cagcaccaac atcatggaac catcatcgcg gctcaacagt tggctgttaa
     8101 acagataggt agacaccgca tccgacacgg aaacctgagt tgccggaact tctatcgcca
     8161 taaaaccgtt cacccgcgtg ccagtaactg tgactggcga gcgaacgcct gttggtggca
     8221 aaacagcacc tggcggttac tcacggcaat cacgtcatta tgaaaaacgc tctggtcgat
     8281 aacgtccggg ttttgctggg cgaaaatcac ctgttgggga ttcacctgat taagccttgc
     8341 caccgcctcg ctggcttcgc gagtctgtcg cgccggataa cgggaaggcc gggtatcatt
     8401 gccttcttct cgcccgtaga caaaaagtag catacccggt tcaccgtaat gaccgccgag
     8461 acgattgtgg tttgccgccc cctcatcacc gagcaacgct acctgcggca acgccgaatg
     8521 gacgctaaat ttctcttcgt ccttaaaaat cgcttttaac agcgattcag tgacgggcgc
     8581 ttccagcgaa cggtgaaatt tattgttcag gttggcaacg gtgagatgca ctttgccatc
     8641 cagcgtatcg gcagatggcg cgatcgttgc cgcattggct acccacattg gcgaagcgga
     8701 actgacgctg gaaagccagt gcggtgcctg gcgtgcaact ttttccagta cctgctcatc
     8761 gctaccactg aatcccaact gacgcagcac cggaataaac ggacgctcgt gcggcggaat
     8821 cacggcctgg gggaatcccg catcggcaag ggctttcatt ttcagtaagc cctgttttgc
     8881 cgccagtcgc gggttagaca cctgaaaacg gtgacgggta gaggcttcat taccaaacga
     8941 caggcccgcg taatgatgcg tcagccctac cagcccgtcg aaattaactt cccaggcgtt
     9001 catcgcacca cctcatcgga aaaatccagc ccggggttaa gcgtggcggg caatgttaac
     9061 gagtccgact ccaggctcgc cataggccat gcgcagtaat ctgcggcata ccaggcgctg
     9121 gggcgatggt taccggaagc accaatgccg ccgaatgggg cggtactggc agcaccagta
     9181 agcggtttgt tccagttaac aatccccgcc cgcgcctcca gcaacagttg atcgaatttt
     9241 tcccgctcgg gggaaaccag accgcaaaag agtccgaagc gagtgttgtt cgccatccga
     9301 atcgcctcat caaaagtatc ataacgccag acgcgcaaaa acggcccgaa cacctcttca
     9361 tccggcacac cgccaacgcc cgtcatttca atgatccctg gtgtcagcaa cgatgtccct
     9421 gcttgtaata agcgcggcgc aagcagggtt cgtccgccca tcgcttccag ttgctgccag
     9481 gcagtaacca cctgctgtgc ggcctgttca gaaatcagcc cgccaataaa cggctgcggt
     9541 tcgtcatccc agttgcccgg cgttaatcgc tggctgacgg caaccagact agcaagaaac
     9601 gcatcgccct gcgctccgct tttgagcaat aaacggcggg cgcaggtgca gcgttgcccg
     9661 gctgtgacaa acgccgactg aatggtcaga tggacagccg cgtcgatatc cgccacctca
     9721 tcgataatta gcggattatt accgcccatc tcaagggcga gaattttctc cggctgaccg
     9781 gagagctggc gatgcagctg gtagccggta ttggcgctac cggtaaacag caaaccgtcg
     9841 agatcctcca gcgcactcag cgcctgaccc gtttcacgcc cgccctgcac caggttcagc
     9901 acgcccggcg gcaagccagc ctgctgccat aaacgcatta ccgcttcgcc actccacggt
     9961 gtcagttcgc tgggtttaaa gataatggtg ttacctgcca gcaatgccgg aacgatatgt
    10021 ccgttcggca aatgaccagg gaaattatac ggcccaaaca ccgccagcac gccgtgcggg
    10081 cgatgtcgca ggctcgccgc accgtccggc atttcactac gctgctcgcc ggtacgaacg
    10141 tgatacgcct taattgatat cgcgatttta ttgatcatcg ccgtcacttc ggttgccgct
    10201 tcccagcgcg gcttacccgt ttctctggca ataatcgcgg ttaattcggc tttattgctt
    10261 tccagcaatc cggcaaagcg ttcgacaacg acctgacgtt cagccaatga gagccgcgcc
    10321 cagcgcggaa acgccgcacg ggctgcccga caagcctgtt cgacctgagc ggcatcggca
    10381 tcattgcctt gccataacac ctcgcccgat accggattac gcttcacacg cgatgcgccc
    10441 tggcccgtta tccagtcacc gttaatccat aaagtcatgc tgttttctcc tctgcgcaca
    10501 ggcgcaccag acgaacgcga tcccctgcgt ggcatttgag ggcatccagt tgtgcggcgg
    10561 ttaaaatcaa acgctcggtt gccggatcgg tacgcgccag caccacgcgg aaatggtgat
    10621 aattttcatt ggcgaccagg caggccggaa aatcgccctg cgcaggctgc ccttctgcca
    10681 cttccaccag ccgactttta cggatggcgc gcacgcggtc gatgtcacac tcaagcgttg
    10741 gcccgccgtc gaagatgtcg atatagttgc ggtaacgaaa accttctttc tccagcaccg
    10801 cgcgggcagg cgcggtttgc ggatgtacct gaccgatgac gtcctgggct tcctgggata
    10861 aaaagtgggt atagatcgga tgtttcggca tcagttctgc aataaatgcc ttttgcccgg
    10921 tgccgcagag aaaatcggcg cggctaaaat ccatcgaaaa gaagcgttta ccgaggcttt
    10981 gccagaacgg tgaatagccg tgttcgtcaa tcaccccgcg catttcagca accactttgt
    11041 cattaaactt gtcgcgaaaa gccgccataa acataaagcg cgatttcgac agcaaatagc
    11101 cgttgccctc tttgcgccag tccgggtcga gaaacagcgt gcacagctcg ctgctgccgg
    11161 tgtgatcgtt actaagaaac agcgtcggca atgcgttata gacattcagc tcttttgagg
    11221 cgtgaaccaa cgtgccgacg cgatagttgt accagggatc gttcagccca accgccacct
    11281 caatagcgca aatccccgcc acggtgcctg tctcgctatc ttccagcacg aacacatagc
    11341 cctgctcgct tttgggcagt tcaccttgcc aggttttgat tgccctttcg atacgcgccg
    11401 aaagcgtggc ttcattggcg ggaagcgacg tcaggccgcc gcccgttttg ctggcaagct
    11461 gcatcagcgc cgagacatct gagcgctcaa cgggacggat gaccatcatg atgaacctcg
    11521 gctaacaaag tgttcgcaag cagctgcaaa gcgatccagt ccggtcgtca cctcttcttc
    11581 gctgacattg agcgcaggcg caaaacgcac cacgttgcca cccgcaatca gtaccatcac
    11641 gcctgctttc gccgcttcca gagagagctg tttcgcttgc ccggcgtaat cggcattcag
    11701 tacacagcca atcaacaaac ctaagccgcg aacttcactg aacaaaccat agcggtgatt
    11761 aatagtatta agacgctcaa caaaccagtc gtgacgctgt ttaacgccat taagcatctc
    11821 tggtgtgttg atgagctcca gcactttgcc tgctaccgcc gaggccagcg gattaccgcc
    11881 ataggtggtg ccatgagtgc caacggtcat cacgcgggcg cactcttcgg ttgccaacaa
    11941 cgcaccgacc gggaaaccgc cgcccagcgc tttggcggtg gttaacagat cgggcgtcac
    12001 gccgtagtgc atataggcat acagctcacc agtgcgcccg acgccggttt gtacctcatc
    12061 aaaaatcagc aaggcattgt ggcggtcaca caattcacgc agaccttgta aaaacgcgtt
    12121 gctggctggc accacaccgc cttcgcccta gatgggctcg acaatcaccg cacaggtggc
    12181 gtcgtcaatc agcgcgctgg cagagttaat atcgttatat gcagcatgac gaatatccgc
    12241 cggcagtggc gcaaaatcct gtgaataggc tggctgccca cccgcactga cagtaaacag
    12301 cgtgcgacca tgaaacgcat ttttgaacgc cacgatgcca ctcttatggc tgccgtagcg
    12361 gtcgtgagcg aatttacgcg ccagttttag cgccgcttcg ttggcttccg caccggagtt
    12421 acaaaagaag acgcgatcgg caaacgtggc gtcgatcaat tttttcgcca gtcgcagtac
    12481 cggctcgttg gtgtaaccgt tgccggtatg ccagaactta ctcgcctgtt cgttcagcgc
    12541 ttcacgcagt tccggatgcg catggcccag cgcgttcacc gcaatgccac ccgcgaagtc
    12601 gatatactct ttcccctgct gatcccacaa gcgcgaacct tcgccacgta ccggtataaa
    12661 gggtgccgga gcgtaaacag gtatcatcca ttcatcaaag ttttcacgcg taattggctg
    12721 agacatagcg acctctacag taaataattc gttatttgta tgttaacaat aagtaatgtt
    12781 tgcgctgtaa atgtagattg cagggttcgt gccagccagt gataaaagtg cataaacggc
    12841 ggaggctaac tggaaatcaa ggagttataa ccaaaccata tgcatttaaa gtgcatataa
    12901 agtgaatacg tttgcgatgt gggtgaataa aaagaataaa aaacgcaatg ttatgcagaa
    12961 gtaaaatata attctagaat tgtgatcatt gacgaaattt actggaaatt actgcgccat
    13021 tctgacgcag cgcgcaccaa aagcgggcat tttttgcgcc atcgttgaca tcattaacaa
    13081 ccatcgatca aatcacttaa caacaggcgg taagcaacgc gaaattctgc taccatccac
    13141 gcactcttta tctgaataaa tggcagcgac tatgaaattt gtctctttta atatcaacgg
    13201 cctgcgcgcc agacctcacc agcttgaagc catcgtcgaa aagcaccaac cggatgtgat
    13261 tggcctgcag gagacaaaag ttcatgacga tatgtttccg ctcgaagagg tggcgaagct
    13321 cggctacaac gtgttttatc acgggcagaa aggccattat ggcgtggcgc tgctgaccaa
    13381 agagacgccg attgccgtgc gtcgcggctt tcccggtgac gacgaagagg cgcagcggcg
    13441 gattattatg gcggaaatcc cctcactgct gggtaatgtc accgtgatca acggttactt
    13501 cccgcagggt gaaagccgcg accatccgat aaaattcccg gcaaaagcgc agttttatca
    13561 gaatctgcaa aactacctgg aaaccgaact caaacgtgat aatccggtac tgattatggg
    13621 cgatatgaat atcagcccta cagatctgga tatcggcatt ggcgaagaaa accgtaagcg
    13681 ctggctgcgt accggtaaat gctctttcct gccggaagag cgcgaatgga tggacaggct
    13741 gatgagctgg ggggtggtcg ataccttccg ccatgctaat ccgcaaacag cagatcgttt
    13801 ctcatggttt gattaccgct caaaaggttt tgacgataac cgtggtctgc gcatcgacct
    13861 gctgctcgcc agccaaccgc tggcagaatg ttgcgtagaa accggcatcg actatgaaat
    13921 tcgcagcatg gaaaaaccgt ccgatcacgc ccccgtctgg gcgaccttcc gccgctaatt
    13981 tagcagctct cctggctcaa actgggtcag gagaattaac cttgagaaaa atcaacaaac
    14041 tgtcagtaat gatttgttgc ctgccgtcct ttgttatacc gtcactgcgt ttttagttgt
    14101 ctgaccactt ctctattatc aagtttgata taggaaactc cacgatgaac gctgagcgta
    14161 actttctgtt tgcctgtctt atttttgcgc tggtcattta cgctatccac gctttcggtt
    14221 tattcgatct gctcaccgat ttaccccact tacagacact cattcgccag agcggattgt
    14281 tcggttatag cctctatatt ctgttattca tcaatgcccc cctctttctg ttaccaggaa
    14341 gcatactggt gatcgctgga ggaatagttt ttggcccgct cttagggaca caactctcat
    14401 taattgccgc aacgctggcc tcctcgtgct cattcctgct ggcgcgctgg ctgggacgtg
    14461 atttactgct gaaatacgtg ggccatagcc ataccttcca ggccattgaa aaaggcattg
    14521 cgcgtaacgg tattgatttt cttattctga cccgcttaat cccgttgttt ccttacaata
    14581 ttcaaaatta cgcttacgga ttcaccacca tcgccttctg gccttatacc cttatttcgg
    14641 cactcacgac cctgcccggt attgttattt ataccgtgat ggcaagcgat ctcgccaatg
    14701 aaggcattac gctgcgcttt attttacaac tctgtctggc gggcctggcg ctgtttattc
    14761 tcatccagct cgcaaaacta ctctacgccc gacacaaaca tgtggatctg tctgcttcgc
    14821 gccgcagccc acttactcac ccaaaaaatg aaggatagaa cgatgtcgca acattattca
    14881 gtgtcatgga aaaaaggact ggctgcactc tgcttactgg ctgttgccgg gcttagcggc
    14941 tgcgatcaaa aagagaatgc ggcggcaaaa gtggaatacg acggactttc gaacagccaa
    15001 ccgctgcgcg tcgatgccaa taaccatacg gtaaccatgc tggtgcaaat taatggtcgt
    15061 ttcctcaccg acgacactcg tcacggtatt gtgtttaaag atggctccaa cggacataaa
    15121 tcgctgttta tggcttatgc gcccccgaaa gcattttatg aagccctgaa agaggcaggt
    15181 ggtacgccgg gcgaaaacat gacgatggat aataaagaaa cgactcatgt cacaggcagc
    15241 aaactggata tttcggtcaa ctggcaaggg gcggcaaaag cgtattcctt cgatgaagtg
    15301 attgttgaca gtaatggcaa gaaactggac atgcgctttg gcggtaattt aacggcagca
    15361 gaagagaaga aaacgggttg cctggtgtgt ctggatagct gcccggtcgg catcgtcagc
    15421 aatgcaacat acacttatgg tgcggtggaa aaacacggcg aagtgaaatt caaaggcaat
    15481 gcctcagttc tcccggcgga taacacgctg gcaacggtta cctttaaaat caccgaataa
    15541 agccaagata aaggatgatg aagatacaat cgcgtaaaat ctggtactac cgtataaccc
    15601 tcatcatcct gttgttcgct atgctgctcg catgggcgct gcttcccggc gtccatgagt
    15661 ttatcaatcg cagcgttgcg gcgtttgccg ccgtggacca acagggtata gaacgcttta
    15721 ttcagtctta cggcgcactg gcggcagttg tctcgttctt gttgatgatt ttacaggcca
    15781 ttgctgcacc gctacctgcg tttttgatca cctttgccaa tgcgtcgctg tttggcgcgt
    15841 tctggggcgg cttgctgtcg tggaccagtt cgatggccgg cgcggcgctg tgctttttta
    15901 tcgccagagt gatgggccgc gaagtggtgg aaaaattaac cggcaaaacc gtgcttgaca
    15961 gtatggacgg ctttttcact cgctacggca aacacaccat cctggtctgt cggttattgc
    16021 cttttgtccc tttcgatcca atcagctatg ctgccggtct gacttcaata cgttttcgct
    16081 cgttttttat cgccaccggg cttggtcaat taccggcgac tattgtttat tcctgggcag
    16141 gcagcatgtt aacaggcggt actttctggt ttgtcaccga actgtttatt ctgtttgccc
    16201 tgaccgtggt gatttttatg gcgaagaaaa tatggcttga gcgccagaag aggaatgact
    16261 gatgggttta ccgccgctta gcaaaattcc tttcatttta cgtccgcagg cgtggctgca
    16321 tcgtcgccat tacggcgagg tgctaagccc gattcgctgg tgggggcgga tcccgtttat
    16381 cttttatttg gtgtcaatgt ttgttgggtg gctggagcgc aaacgctcac cgctcgatcc
    16441 ggtagtgcga tcgcttgtca gcgcgcgcat tgcgcaaatg tgcctgtgtg agttttgcgt
    16501 ggatatcacc agtatgaaag tcgccgagcg taccggcagc accgataaac tgctggcagt
    16561 ggctgactgg cggcaaagcc cgctctttag cgatgaagaa cggctggcgc tggagtacgc
    16621 cgaagccgca agcgtcacac cgccaacagt cgatgatgcc ctgcgtaccc gactggctgc
    16681 gcattttgac gctcaggcgc tcaccgaact gacggcattg atcggcctgc aaaacctgtc
    16741 ggctcgtttt aattctgcca tggacattcc cgctcagggg ctgtgccgta ttccggaaaa
    16801 acgttcttaa ggagggaaga tgcgccattg tgggtggttg ctgggattgt tatcgctgtt
    16861 ttctttggca acacatgcca gtgactggca ggaaattaaa aatgaggcca aagggcaaac
    16921 cgtctggttt aacgcctggg gcggcgatac cgcaattaac cgctatctcg actgggttag
    16981 cggcgagatg aaaacccatt acgctataaa cctgaagatt gttcgtctcg cggatgccgc
    17041 agacgcggtg aagcgcattc agaccgaagc tgctgccggt cgtaaaacgg gcggctcggt
    17101 ggatctgctc tgggtgaacg gcgaaaactt cc
//
LOCUS       DAJTRC010000080        17071 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.080, whole genome shotgun sequence.
ACCESSION   DAJTRC010000080 DAJTRC010000000
VERSION     DAJTRC010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 17071)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17071)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17071
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.080"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..508
                     /locus_tag="OO357_002916"
     CDS             <1..508
                     /locus_tag="OO357_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ParB-like nuclease domain-containing protein"
                     /protein_id="HCR5887790.1"
                     /translation="FREKPVDCVLWVKNSQLMPNDYNPNNVAPPEKKLLQKSIEIDGF
                     TQPIVVTHTDKNAMEIVDGFHRHEIGKGSSSLKLRLKGYLPVTCLEGTRNQRIAATIR
                     HNRARGRHQITAMSEIVRELSQLGWDDNKIGKELGMDSDEVLRLKQINGLQELFADRQ
                     YSRAWTVK"
     gene            complement(509..1669)
                     /gene="ybdL"
                     /locus_tag="OO357_002917"
     CDS             complement(509..1669)
                     /gene="ybdL"
                     /locus_tag="OO357_002917"
                     /EC_number="2.6.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine-oxo-acid transaminase"
                     /protein_id="HCR5887791.1"
                     /translation="MTNNPLIPQSKLPQLGTTIFTQMSALAQQHQAINLSQGFPDFDG
                     PRYLQERLAYHVAQGANQYAPMTGVQALREAIAQKTERLYGYQPDADSDITVTAGATE
                     ALYAAITALVRNGDEVICFDPSYDSYAPAIALSGGIVKRMALQPPHFRVDWQEFAALL
                     SERTRLVILNTPHNPSATVWQQADFAALWQAIAGHEIFVISDEVYEHINFSQQGHASV
                     LAHPQLRERAVAVSSFGKTYHMTGWKVGYCVAPAPISAEIRKVHQYLTFSVNTPAQLA
                     LADMLRAEPEHYLALPDFYRQKRDILVNALNESRLEILPCEGTYFLLVDYSAVSTLDD
                     VEFCQWLTQEHGVAAIPLSVFCADPFPHKLIRLCFAKKESTLLAAAERLRQL"
     gene            1778..2866
                     /gene="hcxA"
                     /locus_tag="OO357_002918"
     CDS             1778..2866
                     /gene="hcxA"
                     /locus_tag="OO357_002918"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxycarboxylate dehydrogenase HcxA"
                     /protein_id="HCR5887792.1"
                     /translation="MPHNPIRVVVGPANYFSHPGSFNHLHNFFTDEQLSRAVWIYGKR
                     AIAAAQTKLPPAFGLPGAKHILFRGHCSESDVQQLAAESGDDRSVVIGVGGGALLDTA
                     KALARRLGLPFVAAPTIAATCAAWTPLSVWYNDAGQALHYEIFDDANFMVLVEPEIIL
                     NAPQQYLLAGIGDTLAKWYEAVVLAPQPETLPLTVRLGINNAQAIRDVLLNSSEQALA
                     DQQNQQLTQSFCDVVDAIIAGGGMVGGLGDRFTRVAAAHAVHNGLTVLPQTEKFLHGT
                     KVAYGILVQSALLGQDDVLAQLTGAYQRFHLPTTLAELEVDINNQAEIDKVIAHTLRP
                     VESIHYLPVTLTPDTLRAAFEKVESFKA"
     gene            complement(2876..3073)
                     /locus_tag="OO357_002919"
     CDS             complement(2876..3073)
                     /locus_tag="OO357_002919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbdD/YjiX family protein"
                     /protein_id="HCR5887793.1"
                     /translation="MFDSLAKAGKYLGQAAKLMIGMPDYDNYVEHMRVNHPDQTPMTY
                     EEFFRERQDARYGGKGGARCC"
     gene            complement(3255..5360)
                     /gene="cstA"
                     /locus_tag="OO357_002920"
     CDS             complement(3255..5360)
                     /gene="cstA"
                     /locus_tag="OO357_002920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation protein CstA"
                     /protein_id="HCR5887794.1"
                     /translation="MNKSGKYLVWTVLSVMGAFALGYIALNRGEQINALWIVVASVCI
                     YLIAYRFYGLYIAKNVLAVDPTRMTPAVRHNDGLDYVPTDKKVLFGHHFAAIAGAGPL
                     VGPVLAAQMGYLPGMIWLLAGVVLAGAVQDFMVLFVSTRRDGRSLGELVKEEMGPTAG
                     VIALVACFMIMVIILAVLAMIVVKALTHSPWGTYTVAFTIPLAIFMGIYLRYLRPGRI
                     GEVSVIGLVFLIFAIISGGWVAESPTWAPYFDFTGVQLTWMLVGYGFVAAVLPVWLLL
                     APRDYLSTFLKIGTIVGLAVGILIMRPTLTMPALTKFVDGTGPVWTGNLFPFLFITIA
                     CGAVSGFHALISSGTTPKMLANEGQACFIGYGGMLMESFVAIMALVSACIIDPGVYFA
                     MNSPMAVLAPAGTADVVASAAQVVSSWGFSITPDTLNQIASEVGEQSIISRAGGAPTL
                     AVGMAYILHGALGGMMDVAFWYHFAILFEALFILTAVDAGTRAARFMLQDLLGVVSPG
                     LKRTDSLPANLLATALCVLAWGYFLHQSVVDPLGGINTLWPLFGIANQMLAGMALMLC
                     AVVLFKMKRQRYAWVALVPTAWLLICTLTADWLKAFSPDAKVGFLVIANKFQAMIDSG
                     NIPSQYTESQLAQLVFNNRLDAGLTIFFMVVVVVLALFSIKTALAALKDPKPTAKETP
                     YEPMPENVEEIVAQAKGAH"
     gene            complement(5541..5954)
                     /gene="entH"
                     /locus_tag="OO357_002921"
     CDS             complement(5541..5954)
                     /gene="entH"
                     /locus_tag="OO357_002921"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proofreading thioesterase EntH"
                     /protein_id="HCR5887795.1"
                     /translation="MIWKRHLTLDELNATSDNTMVAHLGIVYTLLGDDVLEAEMPVDT
                     RTHQPFGLLHGGASAALAETLGSMAGFMMTRDGQCVVGTELNATHHRPVSEGKVRGVC
                     QPLHLGRQNQSWEIVVFDEQGRRCCTCRLGTAVLG"
     gene            complement(5957..6703)
                     /gene="entA"
                     /locus_tag="OO357_002922"
     CDS             complement(5957..6703)
                     /gene="entA"
                     /locus_tag="OO357_002922"
                     /EC_number="1.3.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-dihydro-2,3-dihydroxybenzoate dehydrogenase
                     EntA"
                     /protein_id="HCR5887796.1"
                     /translation="MDFSGKNVWVTGAGKGIGYATALTFVEAGAKVTGFDQAFTQEQY
                     PFATEVMDVADAGQVAQVCQRLLAETERLDVLINAAGILRMGATDQLSKEDWQQTFAV
                     NVGGAFNLFQQTMNQFRRQRGGAIVTVASDAAHTARIGMSAYGASKAALKSLALSVGL
                     ELAGSGVRCNVVSPGSTDTDMQRTLWVSDDAEEQRIRGFGEQFKLGIPLGKIARPQEI
                     ANTILFLASDLASHITLQDIVVDGGSTLGA"
     gene            complement(6703..7560)
                     /gene="entB"
                     /locus_tag="OO357_002923"
     CDS             complement(6703..7560)
                     /gene="entB"
                     /locus_tag="OO357_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis bifunctional
                     isochorismatase/aryl carrier protein EntB"
                     /protein_id="HCR5887797.1"
                     /translation="MAIPKLQAYALPESHDIPQNKVDWAFEPQRAALLIHDMQDYFVS
                     FWGENCPMMEQVIANIAALRDYCKQHNIPVYYTAQPKEQSDEDRALLNDMWGPGLTRS
                     PEQQKVVDRLTPDADDTVLVKWRYSAFHRSPLEQMLKESGRNQLIITGVYAHIGCMTT
                     ATDAFMRDIKPFMVADALADFSRDEHLMSLKYVAGRSGRVVMTEELLPAPIPASKAAL
                     REVILPLLDESDEPFDDDNLIDYGLDSVRMMALAARWRKVHGDIDFVMLAKNPTIDAW
                     WKLLSREVK"
     gene            complement(7574..9184)
                     /gene="entE"
                     /locus_tag="OO357_002924"
     CDS             complement(7574..9184)
                     /gene="entE"
                     /locus_tag="OO357_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2,3-dihydroxybenzoyl)adenylate synthase EntE"
                     /protein_id="HCR5887798.1"
                     /translation="MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVID
                     GERQLSYRELNQAADNLACSLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVL
                     ALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVAEHSSIRVVQLHNDSGE
                     HNLQDAINHPAEGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSAEIC
                     QFTQQTRYLCSIPAAHNYTMSSPGSLGVFLAGGTVVLAADPSATLCFPLIAKHQVNVT
                     ALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIPAEIGCQLQQVFGM
                     AEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFR
                     GYYKSPQHNASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEDIENL
                     LLSHPAVIYAALVSMENELMGEKSCAYLVVKEPLRAVQVRRFLREQGIAEFKLPDRVE
                     CVDSLPLTAVGKVDKKQLRQWLASRASA"
     gene            complement(9194..10369)
                     /gene="entC"
                     /locus_tag="OO357_002925"
                     /pseudo
     CDS             complement(9194..10369)
                     /gene="entC"
                     /locus_tag="OO357_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308659.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismate synthase EntC"
     gene            10661..11617
                     /gene="fepB"
                     /locus_tag="OO357_002926"
     CDS             10661..11617
                     /gene="fepB"
                     /locus_tag="OO357_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe2+-enterobactin ABC transporter
                     substrate-binding protein"
                     /protein_id="HCR5887799.1"
                     /translation="MRLAPLYRNALLLTGLLLSGIAVVQAADWPRQITDSRGTHTLES
                     QPQRIVSTSVTLTGSLLAIDAPVIASGATTPNNRVADDQGFLRQWSKVAKERKLQRLY
                     IGEPSAEAVAAQMPDLILISATGGDSALALYDQLSTIAPTLIINYDDKSWQSLLTQLG
                     EITGHEKQAAERIAQFDKQLAAAKEQIKLPPQPVTAIVYTAAAHSANLWTPESAQGQM
                     LEQLGFTLAKLPAGLNASQSQGKRHDIIQLGGENLAAGLNGESLFLFAGDQKDADAIY
                     ANPLLAHLPAVQNKQVYALGTETFRLDYYSAMQVLERLKALF"
     gene            complement(11621..12853)
                     /gene="entS"
                     /locus_tag="OO357_002927"
     CDS             complement(11621..12853)
                     /gene="entS"
                     /locus_tag="OO357_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin transporter EntS"
                     /protein_id="HCR5887800.1"
                     /translation="MNKQSWLLNLSLLKTHPAFRAVFLARFISIVSLGLLGVAVPVQI
                     QMMTHSTWQVGLSVTLTGGAMFVGLMVGGVLADRYERKKVILLARGTCGIGFIGLCLN
                     ALLPEPSLLAIYLLGLWDGFFASLGVTALLAATSALVGRENLMQAGAITMLTVRLGSV
                     ISPMIGGLLLATGGVAWNYGLAAAGTFITLLPLLSLPELPPPPQPLEHPLKSLLAGFR
                     FLLASPLLGGLLTMASAVLVLYPALADNWQMSAAQIGFLYAAIPLGTAIGALTSGKLA
                     HSARPGLLMLLSTLGSFLAIGLFGLMPMWILGVVCLALFGWLSAVSSLLQYTMLQTQT
                     PEAMLGRINGLWTAQNVTGDAIGAALLGGLGAMMTPVASASASGFGLLIIGVLLLLVL
                     VELRRFRQTPPQVTASDS"
     gene            12964..13968
                     /gene="fepD"
                     /locus_tag="OO357_002928"
     CDS             12964..13968
                     /gene="fepD"
                     /locus_tag="OO357_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe(3+)-siderophore ABC transporter permease"
                     /protein_id="HCR5887801.1"
                     /translation="MSGSVAVTRAIAVPGLLLLLIIATALSLLIGAKSLPASVVLEAF
                     SGTCQSADCTIVLDARLPRTLAGLLAGGALGLAGALMQTLTRNPLADPGLLGVNAGAS
                     FAIVLGAALFGYSSAQEQLAMAFAGALVASLIVAFTGSQGGGQLSPVRLTLAGVALAA
                     VLEGLTSGIALLNPDVYDQLRFWQAGSLDIRNLHTLKVVLIPVLIAGATALLLSRALN
                     SLSLGSDTATALGSRVARTQLIGLLAITVLCGSATAVVGPIAFIGLMMPHMARWLVGA
                     DHRWSLPVTLLATPALLLFADIIGRVIVPGELRVSVVSAFIGAPVLIFLVRRKTRGGA
                     "
     gene            13965..14957
                     /gene="fepG"
                     /locus_tag="OO357_002929"
     CDS             13965..14957
                     /gene="fepG"
                     /locus_tag="OO357_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter permease"
                     /protein_id="HCR5887802.1"
                     /translation="MIYVSRRLLITCLLLISACVVAGIWGLRSGAVTLEISQVFAALM
                     GDAPRSMTMVVTEWRLPRVLMALLIGAALGVSGAIFQSLMRNPLGSPDVMGFNTGAWS
                     GVLVAMVLFGQDLTAIALAAMVGGIITSLLVWLLAWRNGIDTFRLIIIGIGVRAMLVA
                     FNTWLLLKASLETALTAGLWNAGSLNGLTWAKTSPSAPIIILMLIAATLLVRRMRLLE
                     MGDDTACALGVSVERSRLLMMLVAVVLTAAATALAGPISFIALVAPHIARRISGTARW
                     GLTQAALCGALLLLAAVLCAQQLFMPYQLPVGVVTVSLGGIYLIVLLIQESRKK"
     gene            14954..15769
                     /gene="fepC"
                     /locus_tag="OO357_002930"
     CDS             14954..15769
                     /gene="fepC"
                     /locus_tag="OO357_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-enterobactin ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5887803.1"
                     /translation="MTESVARLRGEQLTLGYGKYTVAENLTVEIPDGHFTAIIGPNGC
                     GKSTLLRTLSRLMTPAHGHVWLDGEHIQHYASKEVARRIGLLAQNATTPGDITVQELV
                     ARGRYPHQPLFTRWRKEDEEAVTKAMQATGITHLADQSVDTLSGGQRQRAWIAMVLAQ
                     ETAIMLLDEPTTWLDISHQIDLLELLSELNREKGYTLAAVLHDLNQACRYASHLIALR
                     EGKIVAQGAPKEIVTAELIERIYGLRCMIIDDPVAGMPLVVPLGRTAPSTANS"
     gene            complement(15766..16899)
                     /gene="fepE"
                     /locus_tag="OO357_002931"
                     /pseudo
     CDS             complement(15766..16899)
                     /gene="fepE"
                     /locus_tag="OO357_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
BASE COUNT         3987 a         4860 c         4442 g         3782 t
ORIGIN      
        1 ctttcgtgaa aagccagtgg attgcgttct gtgggttaaa aacagccaac ttatgcctaa
       61 tgattacaac ccaaacaacg tggcaccacc cgaaaaaaaa ctgctgcaaa aatccattga
      121 aatagatggt tttacccaac ccattgtggt cacacatacg gataaaaatg ccatggaaat
      181 cgtggatggt tttcaccgcc atgaaattgg taaaggctca tcatcattaa aattacgctt
      241 aaaaggttat ttgccggtga cctgtctgga aggtacgcgt aatcaacgca tcgccgcgac
      301 tattcgtcat aatcgcgctc gtggacgcca tcaaatcacc gccatgtcgg aaatcgtccg
      361 tgaacttagc cagttgggat gggatgataa taaaattggc aaagagctgg gaatggacag
      421 cgacgaagtg ctgcgactga agcaaattaa cggcctgcaa gagttgtttg ccgaccgtca
      481 atattcccgc gcctggacgg ttaaatagct aaagctggcg caggcgttca gctgctgcca
      541 gcaacgtcga ttccttcttg gcaaaacaga gacgaatcag tttatgtggg aagggatcgg
      601 cgcaaaacac cgacagcgga atcgccgcta cgccgtgctc ctgcgtcagc cactggcaaa
      661 actcaacatc atccagggta gaaaccgcgc tgtaatccac cagcaaaaag tatgtacctt
      721 cacacggtaa aatctccagc cggctttcat ttaaagcatt caccagaata tcgcgcttct
      781 ggcgataaaa gtccggtaac gcaagataat gctcaggttc tgcacgtagc atatcagcaa
      841 gcgccagctg tgccggggta ttcaccgaaa aggtcagata ctgatgtacc ttgcgaattt
      901 cggcgctgat gggcgctggc gcaacacaat aacccacttt ccagccggtc atatgatagg
      961 tcttgccaaa tgaagaaacc gccactgccc gctcacgcag ctgcggatgc gccagcacac
     1021 tggcatggcc ctgttgtgaa aagttgatgt gctcgtagac ttcatcgcta atgacaaaaa
     1081 tctcgtgccc ggcgatcgcc tgccacaaag cggcgaaatc agcctgctgc cagacagttg
     1141 cactggggtt atgcggagtg ttgaggatca ccagtctggt gcgctcgctt aataatgcgg
     1201 caaattcctg ccagtcaacg cgaaaatgcg gtggttgcag tgccatacgt ttcactattc
     1261 ccccagaaag cgcgatggcg ggggcgtaac tgtcatagct gggatcaaaa caaatcactt
     1321 catcgccatt gcgcaccagt gcggtaatcg ccgcgtataa cgcttccgtc gcccctgccg
     1381 ttacggtgat atcgctatcg gcatctggtt gatagccata caaacgttcc gttttctgag
     1441 caatcgcctc gcgcaaggcc tgcacgccgg tcatgggcgc gtattggttt gccccctggg
     1501 caacgtggta cgccagccgc tcctgtaaat agcgcggacc atcaaaatca ggaaagcctt
     1561 gcgacaggtt aatcgcctgg tgttgctgcg ccagcgcgct catctgggtg aaaatagtgg
     1621 tgccaagttg tggaagtttg ctttgtggaa tcagagggtt atttgtcatt tttattgtac
     1681 ctgacgaagt tgcgtgttga aggcactata acacgatgcc agtatttggc aatcaagacg
     1741 tttagatgtc taaataaaac aataaggaca acacaacatg cctcacaatc ctatccgcgt
     1801 ggtcgtcggc ccggctaact acttttcaca tccaggaagt ttcaatcacc tgcacaattt
     1861 tttcactgat gaacaacttt ctcgcgcggt gtggatctac ggcaaacgcg ccattgctgc
     1921 ggcgcaaacc aaacttccgc cagcgtttgg acttccaggg gcaaagcata ttttgtttcg
     1981 tggtcattgc agcgaaagcg atgtacagca actggcggct gagtccggtg acgatcgcag
     2041 cgtggtgatt ggcgtcggcg gcggcgcact gctcgacacc gcgaaagccc tcgcccgccg
     2101 tctcggtctg ccatttgttg ccgctccgac gatcgccgcc acctgcgcag cctggacacc
     2161 gctctccgtc tggtataacg atgccggaca ggcgctgcat tatgagattt tcgacgacgc
     2221 caattttatg gtgctggtgg aaccggagat tatcctcaat gcaccgcaac aatatctgct
     2281 ggcggggatc ggtgacacgc tggcgaaatg gtatgaagcg gtggtgctgg ctccgcaacc
     2341 agaaacgttg ccgctaaccg tgcggctggg gatcaataat gcgcaggcca ttcgcgacgt
     2401 cttgttaaac agtagcgaac aggcacttgc cgatcagcaa aatcagcagt taacgcaatc
     2461 attttgcgat gtggtggatg ctattattgc tggtggtggg atggtcggtg gtctgggcga
     2521 tcgttttacg cgtgtggcgg cggctcatgc cgtgcataac ggtctgactg tcctgccgca
     2581 aaccgagaag tttcttcacg gcaccaaagt cgcttacgga attctggtgc aaagcgcctt
     2641 gctgggtcag gatgatgtgc tggcgcaatt aaccggggcg tatcagcgtt ttcatctgcc
     2701 gactacgctg gcggagctgg aggtggatat caataatcag gcggagatcg acaaagtgat
     2761 tgcccacacc ttgcgcccgg tggagtccat tcattacctg ccagtcacgc tgacaccaga
     2821 tacgttgcgt gcagcgttcg aaaaagtgga atcgtttaaa gcctgacgtg caaatttagc
     2881 agcagcgcgc gccgcctttt ccaccgtagc gcgcgtcctg ccgctcacgg aaaaactcct
     2941 cgtaggtcat cggcgtttga tcgggatgat taacccgcat atgttcgaca tagttgtcgt
     3001 aatcaggcat accaatcatc agcttcgccg cctgacctaa atattttccg gcttttgcca
     3061 gtgaatcaaa catcttggcc tccgctaacc atgttgctga aagagtgccg gatgcggcgt
     3121 gaacgcttta tccggcctac aaatgctatc aacgcactcg gtcagtcccc tcgccccatc
     3181 ggggagaggg ttagggtgag ggggaaccct cggcactatg ccaacattcg ccaacatccc
     3241 ccctcactct gactttagtg cgcgcctttc gcctgcgcta cgatctcctc gacattttcc
     3301 ggcatcggct catacggcgt ttctttcgcc gttggcttcg gatctttcaa tgccgcaagt
     3361 gccgtcttaa tcgagaacag tgccaggacc accacgacca ccataaagaa gatggttaac
     3421 ccggcatcca gacggttgtt gaacaccagt tgcgccagct gtgactcagt atactgcgac
     3481 ggaatattgc cgctgtcgat cattgcctgg aacttgttag caatgaccag gaagccgact
     3541 ttcgcatccg ggctaaacgc tttcagccag tcagcggtca gggtacaaat cagcagccag
     3601 gccgttggta ccagcgccac ccaggcgtaa cgttgacgtt tcatcttgaa caacaccacg
     3661 gcacagagca tcagcgccat ccctgccagc atctggttgg caataccaaa cagcggccac
     3721 agagtgttaa taccgcccag cggatcgacc acgctctgat ggaggaagta gccccacgcc
     3781 agtacgcaca gcgctgttgc cagcaggtta gcaggcagtg aatcggtccg cttcaggcca
     3841 ggagacacca cgcccagcag atcctgcaac ataaagcgcg cagcacgcgt acccgcatct
     3901 accgccgtca gaataaacag agcttcaaac agaatggcga agtgatacca gaacgccaca
     3961 tccatcatac cacccagcgc accgtgcagg atgtaagcca tccccaccgc caacgtcggc
     4021 gcaccgcccg cacgggaaat gatcgactgt tcacccactt cgctggcaat ctggttgagc
     4081 gtatctggtg taatgctaaa gccccagcta ctcaccacct gcgcggcaga agcgactaca
     4141 tccgccgtcc ctgccggagc cagcaccgcc attgggctgt tcatggcaaa atacacgccc
     4201 ggatcgatga tacaggcaga aactagcgcc ataatcgcca cgaaggattc catcaacatc
     4261 ccaccgtagc cgataaagca cgcctgccct tcgttcgcca gcatcttcgg cgtggtgccg
     4321 gaagagatca gcgcatggaa gccagacacc gcaccacagg cgatggtgat aaacaggaac
     4381 gggaacaggt taccggtcca taccgggcca gtgccatcga caaatttggt cagcgcaggc
     4441 atggtcagcg tcgggcgcat aatcaaaatg cctaccgcca gaccgacgat agtcccgatt
     4501 ttcaggaagg tcgagagata gtcacgcggg gccagcagca accacaccgg cagcaccgcc
     4561 gccacaaaac cgtagcccac cagcatccag gtcagctgca cgccggtaaa gtcaaagtac
     4621 ggtgcccagg tcggactttc tgccacccag ccgccagaga taatggcgaa aatcaggaac
     4681 accaggccaa tcactgacac ttcgccaatg cggcccggac gcagatagcg caggtagatt
     4741 cccataaaaa tcgccagcgg aatggtgaac gcaacggtat acgttcccca cgggctatga
     4801 gtcagggctt tcaccacgat catcgccagt actgcaagga taatgaccat gatcataaag
     4861 caggctacca gcgcaatcac cccggcagtt ggccccatct cttctttgac cagctcaccc
     4921 agcgagcgac cgtcacggcg cgtagaaaca aacagcacca tgaaatcctg caccgcaccg
     4981 gcgagaacca ccccggcgag cagccagatc atccccggca ggtagcccat ttgcgctgcc
     5041 agcaccggcc ccaccagcgg ccctgctccg gcaatggccg caaaatggtg accgaacagc
     5101 actttcttgt ccgtcggcac atagtccagc ccgtcgttat ggcgcaccgc tggcgtcata
     5161 cgcgtcgggt caaccgccag cacatttttg gcgatataca gcccataaaa acggtaagcg
     5221 atcagataga tacagaccga cgccaccaca atccacagcg cgttgatctg ttccccacga
     5281 tttaaagcaa tgtatcccag agcaaatgct cccattacag agagcactgt ccagacgagg
     5341 tatttccctg atttgttcat agttgttatc cgtgtgcgtg tccatagaga tgttacattt
     5401 tgtttctata acatctttgt acgatttaac aacacggtaa ccatgtaaat cggagttgaa
     5461 accaatattt aacttaacaa tgttaactcg atcactccgt taaccgagtt ttcctgcaat
     5521 ctcatttaat tctgtcgggc tcatcccaaa actgccgtac ccagccgaca agtgcagcaa
     5581 cgccgcccct gttcatcgaa aacgacgatt tcccaactct gattttgcct cccaagatgc
     5641 agcggctggc agacgccgcg taccttgcct tcagacaccg ggcgatgatg ggtggcgtta
     5701 agttccgtgc ccaccacaca ctgcccgtca cgggtcatca taaatccggc catcgatccc
     5761 agcgtttcag ccagcgccgc cgacgcgccg ccatgcagca ggccaaacgg ctgatgagta
     5821 cgggtatcaa ccggcatttc ggcttccagc acatcatctc ccagaagggt atacacaatt
     5881 cccagatgcg ccaccattgt gttatcgctg gtggcgttca gttcgtcgag cgttaaatgg
     5941 cgtttccaga tcatgcttat gcccccagcg ttgagccgcc atcgaccaca atatcctgta
     6001 gggtgatatg gctggcgagg tcagaggcga ggaacaaaat cgtgttggcg atctcttgtg
     6061 gacgggcgat tttccccagc ggaatgccga gtttaaactg ctcgccaaag ccgcgaatac
     6121 gctgttcttc ggcgtcatcg ctcacccaca gcgtgcgttg catatcggtg tcggtggagc
     6181 caggcgaaac cacattacag cgcacgccgc tacccgccag ttccagcccg acgctcaacg
     6241 ccaggctttt cagcgccgct ttcgatgcgc cataagcact cataccaata cgcgccgtgt
     6301 gtgcggcgtc ggacgccaca gtgacaatcg ccccgccccg ctgacggcga aactggttca
     6361 tggtttgctg gaacaggtta aacgcaccgc cgacgttaac cgcaaaagtc tgctgccagt
     6421 cctctttact gagctgatcg gtcgcgccca tgcgcaaaat tcccgccgca ttgatcagca
     6481 cgtccagtcg ctccgtttca gctaacagtc gctgacatac ctgcgccacc tgcccggcgt
     6541 cggcaacatc cattacttcg gtcgcaaagg gatattgctc ctgagtgaac gcttgatcaa
     6601 aacctgtaac tttcgctccc gcctcaacaa acgtcagcgc cgtggcgtag ccgatacctt
     6661 tacctgcgcc ggttacccag acatttttac cgctgaaatc cattatttca cctcgcggga
     6721 gagtagcttc caccaggcgt cgatggtcgg gtttttcgcc agcatgacaa agtcgatatc
     6781 accatgcact ttgcgccagc gcgccgccag cgccatcatg cgcaccgaat ccagaccgta
     6841 gtcgatcagg ttgtcgtcat cgaacggttc atcggactcg tccagcaacg gcaggatcac
     6901 ctcacgcagc gccgctttgc tggcggggat aggtgctggc agtaattctt cggtcatcac
     6961 cacccggcca gaacgtccgg ccacatattt cagcgacatc aaatgctcgt cacggctgaa
     7021 atcagccagc gcatccgcca ccataaacgg tttaatatcg cgcataaatg cgtcggttgc
     7081 ggtggtcata cagccaatgt gggcatatac tcctgtaata atcagctggt tacgtccact
     7141 ctctttgagc atttgctcca gcggagaacg atgaaacgcg ctgtagcgcc acttcaccag
     7201 caccgtgtcg tcggcatctg gcgtcaggcg atccaccacc ttttgctgtt ccggcgagcg
     7261 ggtcaggccc ggcccccaca tatcattcaa cagcgcccga tcttcatcgc tctgctcttt
     7321 cggctgggcg gtgtaataaa ccgggatatt gtgctgtttg cagtagtcgc gcagcgcagc
     7381 aatattcgcg atcacctgct ccatcatcgg gcagttctcg ccccagaagc tgacaaaata
     7441 gtcctgcata tcatggatta acaacgcggc acgttgcggt tcaaaggccc agtcaacttt
     7501 attctgcgga atatcgtgag actccggcag tgcgtaagcc tgtaattttg gaatagccat
     7561 cgtgttctct ccttcaggct gatgcgcgtg acgccagcca ctgacgtaat tgttttttat
     7621 cgactttccc gaccgccgtc agcggaagtg aatccacaca ctccacgcga tccggtaatt
     7681 taaattcggc aataccctgt tcacgcagga aacgacgcac ctgcaccgcg cgcagcggct
     7741 cttttaccac cagataagca cagctttttt cgcccatcag ctcattttcc atgctcacca
     7801 gtgcggcata gatcactgcc ggatggctca gcagcaggtt ttcgatatct tcggcagcga
     7861 tcttctcgcc gccacggttg atctgatctt tctcgcgccc ctgcacggtg atgtaaccct
     7921 ctggatcaat agagatcaga tcgccggaac agtaaaaacc gttggcatca aaggcactgg
     7981 cattgtgctg cggacttttg taatagccac ggaaggtgta cggcccgcgc gtcatcaggc
     8041 gtccaacttc cccttgcggc agtggatttc cttcggcatc ggcaacccac acttcatcgt
     8101 ccggacacat tgggtaaccc tgggtatgga taattttctc cgcgctatca tcaagacggg
     8161 tgtagttcac cagcccttcc gccatgccaa acacctgctg caactggcag ccaatctcag
     8221 cgggaatacg cgctgcaagc gtggcagaaa gacgtgcgcc gccgacctgt aacagtttca
     8281 gcgaggcaag ctgcgcccgg ctttcgcctt cagccagcgc ctgcaaccac aggctgactg
     8341 ccggcggcac cagcgcggta acgttaacct gatgttttgc aatcaacggg aagcaaagcg
     8401 tggcgctggg atcggccgcc agaacaaccg ttcctccggc aagaaagacg cccagcgatc
     8461 ctggcgaact catagtgtag ttatgagccg ccgggatcga gcacaggtag cgcgtctgtt
     8521 gtgtgaactg acaaatctcg gcgctacgac gcacgctgta gtaatagtcg ttatgagtgc
     8581 gcgggatcag tttcggtgtg ccggtggtgc cgccggaaag ctggaaatag gccacttcat
     8641 cagcaggtga tggcgtggca gtaaaaccct ctgccggatg gttaatcgca tcctgcaagt
     8701 tatgctcacc gctgtcgttg tgcagttgca ccacgcgaat ggaagaatgc tctgcaacaa
     8761 atgtgttgag gaaatcatcc ccgctaaata gcgcatgctg gcgatcggca atcagtaacg
     8821 ccggttcaat ctggctggcg taggcgttca gttcactacg ctgatggctg aacagcgcca
     8881 gcaccggcgc gacgcccagt ttcagcagcg cgaaaaaggt aatgtaaagt tcagcgacgt
     8941 tacccagttg taccagcgcg gtttcaccag gtttaatgcc ctgacggcgc aaactacagg
     9001 cgaggttatc cgccgcctga ttcagctccc gataactcaa ctgccgctcg ccgtcgataa
     9061 ccgcgatgct gtcactcgca gcgtggcgag tcagaatgtc ggtcagtggc aaatcctgcc
     9121 agtagccttt ttcccgatag cgacgggcaa actcttccgg ccagcgggtg aatggaatgc
     9181 tcatcctcgc tccttaatgc aatccaaaaa cgttcaacat ggtagaaagt ttgacgcctg
     9241 tttcgcgcca ctcacccaac ggtgacgacg caggcacaat ccccgctccg gcaaacagac
     9301 gcacctgatt ttcccgcagc ttcgcgcagc ggatggtcac cacccattcg ccgttacctt
     9361 cgctgtcaca ccaacccaca atgccgccaa acagttcgcg gtcgaacggc tccagttcag
     9421 caataacctg ggtcgcggcc tgatgcggga aaccgctcag cgcgggggtc ggatgcagca
     9481 gacaggccag agtcagtgcg ttttcttgcg aattcgcttt accttcaaag ggagttgcga
     9541 gatgccacag cgtcggcgtg gtaatcaatt gtggagagga gggaacgtgt aactcactac
     9601 tgcgttcgcg cagtacctct ttcatcgcct gagtccccag ttcatgttca tggcgatctt
     9661 tttctgacgc cagcagacga ttacccgctt cgcgatcgag cacttcatcc ggctgacgac
     9721 gcgcggaacc ggctaacgga atggagctaa aacgctcgcc gtctttacgt agcagcagtt
     9781 ccgggctggc ccccagcagg acgccaccat cagccagcgg gacatggaag ttgtaactaa
     9841 ccgggttttg cgcaatcaac cgttccagca atacgccact atcaatggcg gcgtcagtgg
     9901 tgatatcaat caatcgtgac aacaccactt tgtcgacctg cggcgtggcg gtaagtgcgg
     9961 cggcgcgggc acccatctgt tcaaacgtgg tttgttcagg aattgcctag cgttccacca
    10021 cgttcagcga ctggctgcgg gtaaaacagc gggctgaggt ctgtttttcc tgacgggaga
    10081 acgactgcta ggattcggga atatacagcg acgaaggctg acgtggatcg aagggaatcg
    10141 ccccgaccat caccggattt ttgatgccct gcgctttggc atcggcaaac agcgcggcga
    10201 gtttttgctg gaagggactg tcgggcgaat ccccgttcac agccggttca tcgaaacggg
    10261 cgaaacatcc tgacgtcgta aaactgcggt acggcgacat aaagaaaaag cgattgggcg
    10321 caagtgttgc catggtctgc tgtacttcct cagccagtga cgtatccata tcatcctcca
    10381 caaaatgata aaggctttaa taatgattat cacttatatt ttcggccgct aacctaaaag
    10441 caaacgcgca ctatgtcaac tcttgaggta acgcaatctg caactcgggg ttgcatctgg
    10501 ttgtggttac aatgaaaatg agaagcatta ttgatgaatt cgcataagcg caatgtgatg
    10561 tcctgcgccg ttttgccccc tctccccaaa gggacggatt gtgcgctttg tcgaatttgt
    10621 cattacgccc ttaaccttat taataacagg aagctgactt gtgagactcg ccccgctcta
    10681 ccgcaacgcc cttctattaa caggactttt gctttcagga atagccgtag ttcaggccgc
    10741 cgactggccg cgtcagatta ctgacagccg gggcactcat accctggaaa gccagccgca
    10801 gcgtattgtt tccaccagcg tcaccctgac cggatcactg ctggcgattg atgctccggt
    10861 gatcgccagc ggcgcgacca cgccgaataa ccgcgtcgca gatgaccagg gctttttacg
    10921 ccagtggagc aaggtggcga aagaacgcaa actgcaacgg ctctatatcg gcgaaccgag
    10981 cgccgaagcc gttgccgcgc aaatgccgga tctgatttta attagcgcaa ccggcgggga
    11041 ttcggcgctg gcgctgtatg atcagctttc caccatcgcc ccgacattaa tcatcaatta
    11101 cgacgacaaa agctggcagt cgctgttaac gcagcttggc gaaattaccg ggcatgagaa
    11161 acaagcggca gagcggattg cacagtttga taagcaactg gcagcggcga aagagcaaat
    11221 caaattaccg ccgcagccgg tcactgccat tgtctatacc gccgctgcac acagtgccaa
    11281 tctctggacg ccggaatcag cacaagggca gatgctggaa caactcggct ttacgctggc
    11341 gaaattgccc gcagggttaa acgccagcca aagccagggc aaacgccatg acatcattca
    11401 gcttggtggg gaaaatctgg ctgcggggtt aaacggtgaa tcgctattcc tgttcgctgg
    11461 tgatcagaaa gatgccgatg cgatttatgc caatccactg ctcgcgcacc tgcctgcggt
    11521 acaaaacaag caggtttatg cgctgggaac cgagacgttc cgcctggatt actacagcgc
    11581 catgcaagtg ctggaacggc ttaaggcgct gttttaagca ttaactgtcg gacgctgtca
    11641 cctgcggcgg cgtctggcga aaacgtcgca actccaccag caccagcaat aacaacacgc
    11701 cgataatcaa caaaccaaaa ccgctcgcgc ttgcggaagc aaccggtgtc atcatcgcgc
    11761 ccaaaccacc cagcagcgcc gcgcctatgg catcgcccgt cacgttctgc gccgtccaca
    11821 aaccgttaat ccgccctaac atcgcttccg gggtttgcgt ttgcagcatt gtatattgca
    11881 gcaacgaact aaccgcgctc agccagccga acagcgccag acaaaccacg cctaaaatcc
    11941 acatcggcat caggccaaac agaccaatgg cgaggaacga tcccagcgtg gagagcagca
    12001 tcaataaccc tggtcgcgca ctatgtgcca gcttcccgct ggttaacgca ccaatagccg
    12061 tgccgagcgg gatcgccgcg tagagaaaac caatctgtgc cgctgacatc tgccagttgt
    12121 cagccagcgc cggatacagt accagcaccg cgctcgccat cgtcaataaa ccacccagca
    12181 gcgggctggc aagcagaaaa cgaaatcctg ccagtaatga tttcaacgga tgctcaagcg
    12241 gctgcggtgg cggtggcaac tccggaaggc ttaataacgg taacaaggta ataaacgtgc
    12301 ccgccgccgc cagcccgtag ttccaggcta cgccgccggt cgccagcaat aaaccgccaa
    12361 tcatgggcga aatcaccgac cccaggcgca cggtcaacat ggtgatcgcc ccggcctgca
    12421 ttaagttttc acgccctacc agtgcagatg ttgccgccag tagcgccgta acaccaagtg
    12481 atgcgaaaaa accatcccat aaaccaagta aatagattgc cagcaatgac ggctccggca
    12541 gcagtgcatt aaggcacagt ccaatgaagc caatgccaca ggtgccgcgc gccagcaaaa
    12601 tcactttttt gcgttcatag cgatccgcca gcacaccgcc aaccatcagg ccaacaaaca
    12661 tcgcgccgcc ggtcagcgtc accgaaagcc ccacctgcca ggtagaatgg gtcatcatct
    12721 ggatctgcac cggcaccgcg acgccgagca aacccagaga cacaattgag ataaaacgag
    12781 cgaggaatac tgcgcgaaac gccgggtgcg ttttcaacag gctgaggtta agcagccagg
    12841 attgtttatt cattacaatg ccttgccatc aaatatttta aatacctatt cttaggctgc
    12901 acatgttaac atatccaaat aagatcgata acgataatta atttcattat catggaagtt
    12961 cgtatatctg gttctgttgc cgtgacacgc gccattgccg tgcccggatt gctgttatta
    13021 ctgattatcg cgacggcatt aagcctgctc attggggcaa aatcactccc cgcttccgta
    13081 gtgctggagg ccttctccgg cacctgccag agcgccgact gcaccatcgt gctcgacgcg
    13141 cggctgccgc gtacccttgc cggtttactg gcaggcggcg cgcttggcct tgccggggcg
    13201 ttaatgcaaa ccctcacccg aaacccactt gccgaccccg gcttgcttgg cgtgaacgcc
    13261 ggagccagct ttgccattgt gctgggtgcg gcgctgtttg gttactcatc cgcacaggaa
    13321 caactggcga tggccttcgc cggggcgctg gtggcctcat tgattgttgc ctttaccggc
    13381 agtcagggtg gagggcagtt aagtccggtg cgtttaaccc tggcgggcgt ggcgctggcg
    13441 gcggtgctgg aaggactgac cagcggcatc gccctgctta atcctgacgt ctacgatcaa
    13501 ctgcgtttct ggcaagccgg ttcgctggat attcgcaatc tacatacctt aaaagtggtg
    13561 ctgatcccgg ttctgatcgc cggagcaact gcgctattac tgagtcgcgc gctgaacagt
    13621 ttgagcctcg gcagtgacac cgcgacggcg ctgggcagtc gcgtggcgcg cacacagttg
    13681 attggtctgc tggcgattac cgtgctttgt ggtagtgcga cggcggtagt tggcccgatt
    13741 gcctttatcg gcctgatgat gccgcacatg gcgcgctggc tggtaggtgc cgatcatcgc
    13801 tggtcgctgc ccgtcacgct acttgctacc cctgccctgc tgctgtttgc cgatatcatc
    13861 gggcgggtga ttgtgcccgg cgaactgcgc gtttctgtgg tcagtgcgtt tattggtgca
    13921 ccggtgctga tcttcctcgt gcgacgtaaa acgcgaggtg gtgcatgatt tacgtctctc
    13981 gccgattact catcacctgt ttgctgctga tttccgcctg tgtggttgca ggtatctggg
    14041 gattacgcag cggtgccgtc acgctggaaa tctcgcaggt attcgccgcg ttgatgggcg
    14101 atgcgccgcg cagtatgacg atggtggtca ccgaatggcg tttaccacgc gtgctgatgg
    14161 cgctgttgat tggcgcggcg ctgggcgtca gcggcgcgat ttttcagtcg ctgatgcgta
    14221 acccgctcgg cagccctgac gtaatgggct ttaacaccgg ggcgtggagc ggcgtgctgg
    14281 tggcgatggt gctgtttggt caggatctga cggctatcgc gctggcagca atggtgggcg
    14341 gcattatcac ttcgctgttg gtctggctgc tcgcctggcg taacggtatc gacacctttc
    14401 ggttgattat tatcggtatc ggcgttcgcg ccatgctggt ggcctttaat acctggctgt
    14461 tgctgaaagc gtctttagaa acggcgctaa cagcaggttt gtggaatgcc ggatcgctca
    14521 acggcctgac gtgggcaaaa acctcgcctt ccgcacccat cattatattg atgctcattg
    14581 ccgccacctt gctggtgcgg cgaatgcgct tgctggagat gggcgatgat accgcctgcg
    14641 cgctgggcgt cagcgtcgag cgttcgcgtc tgttaatgat gctggttgca gtggtgctta
    14701 ccgctgcggc aacggcgctt gccgggccga tttcctttat tgctttagtc gcgccgcaca
    14761 ttgcccgacg cattagcggc accgctcgct gggggctgac ccaggcggcg ctgtgcggag
    14821 cgctgttact gctggcggcc gttctctgcg cccaacaact gtttatgccg tatcaacttc
    14881 cggttggcgt cgttaccgtc agcctcggcg gtatttacct tatcgtcttg ttaattcagg
    14941 agtctcgcaa aaaatgaccg aatcagtagc ccgtttgcgc ggcgaacagt taaccctggg
    15001 atatggcaaa tataccgttg cggaaaatct gactgtagaa attcctgatg gtcacttcac
    15061 ggcaattatc ggaccaaatg gctgtggtaa atccacgtta ctgcgtacct taagccgcct
    15121 gatgacgcct gctcacgggc atgtctggct ggatggcgag cacattcaac attacgccag
    15181 taaagaagtt gcacgccgga ttggtctgtt ggcgcaaaat gctaccacgc cgggcgatat
    15241 caccgtgcag gagctggtgg cgcgtggacg ttatccgcat caaccgctgt ttacccgctg
    15301 gcgcaaagag gatgaagaag cggtaacgaa agcgatgcag gccacgggaa taactcatct
    15361 ggcagatcaa agcgtggata ccctttctgg cggacaacgc cagcgagcgt ggatcgcgat
    15421 ggtgctggcc caggaaacgg caattatgct gctcgacgaa cccacgacct ggctggatat
    15481 cagtcatcag attgatctac tggaactgtt aagcgaactg aaccgcgaga aaggctatac
    15541 cctggcggcg gtgctgcacg atcttaatca ggcctgtcgt tacgccagcc atttgattgc
    15601 attgcgggaa gggaaaattg ttgctcaggg agcgccgaag gagattgtca ctgctgaact
    15661 gattgagcgc atttatggtc tgcgatgcat gatcattgac gatccggtag ccggaatgcc
    15721 gcttgtggtg ccgctcggac gaacggcacc ttcaaccgca aatagttaaa ctaagtggtc
    15781 tgccatcatg gcatcctgtt ttctggatgc catcgcatag cgcaataaca cgctaccaca
    15841 agccaccatc ccgccgatca gcgcggaaag gatcacaata atcgccttac ccggaccgtc
    15901 ttttttcact ggcaatgacg ggcttaactg atatttaaac ggcgtaaaat tcacatcgtt
    15961 gacatttgct tttgttaatt gctcgacaag atactgccga ttacgtaatt caccgttcag
    16021 ttccgcaacg tcagtaaccg ctttttcaat ttccagtttg cgttcaatac cgtctgcgcc
    16081 gagggaaata gagaaatcgg ggtcatcttt aacggcctga ccattactgt atacgggctt
    16141 tttaattcct gccgcgttgg caatgtcgag tgaataattg aggcgctgaa tgtttgcatc
    16201 aagttgattt ttcgttttaa tgcgatcctg agccagtttt tctttttcaa actgggtttt
    16261 gatctccagt ttattacgga cgttttctat cgactctttc accaccaacg cagagatata
    16321 atcgatatac ccgctcaaaa cggtctgcgc ctctttactg gttggcgcgg taaaacttag
    16381 cgtccaggat gtatacagtg acggttcatc ttttttctta ctagcattgt catcaaccgc
    16441 tttcattttt tcgctcaatg caacaatagc gcgatgcaaa tccagttcat cgattttcgc
    16501 ctcttttaat tagtccatca cataaggtga tgaacgcagg tactcttcca gcaagctaac
    16561 agactgaaac ttcttgataa acaggttaaa tgcaccgcca cggtcgatat taacatcgag
    16621 atcaagaacg cggagtttgg taaaggtttt ctccaggtcc tgccactgaa tggcttcggc
    16681 gggagtaata acggctgagc tggtccattt ttgcggcagg atgaaagaga tcagtaaacc
    16741 tgcgcaggca aatgcaaaaa cgaccgccat gaccgttttt ttggcccgcc ataaaacttc
    16801 gattagatta agtaaatcaa tttcattatt actgggcgac gccagaggat actcagaaaa
    16861 atgagcgtca cttccctgtt taatattcaa tgatgacatg gggtataacc tgaaaatgag
    16921 tccaataggg gaaaatctcc acggcgctca ctttaccagt tggtgagaat tatccgaagc
    16981 tgaagtttgt aaattccttc cactgaatgc aacattcact ctatttaaga gaaatattca
    17041 tcaaacctat aaatcaaaca aataaaaaac c
//
LOCUS       DAJTRC010000081        16564 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.081, whole genome shotgun sequence.
ACCESSION   DAJTRC010000081 DAJTRC010000000
VERSION     DAJTRC010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16564)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16564)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16564
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.081"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..517
                     /gene="mntP"
                     /locus_tag="OO357_002932"
     CDS             <1..517
                     /gene="mntP"
                     /locus_tag="OO357_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="manganese efflux pump MntP"
                     /protein_id="HCR5887804.1"
                     /translation="FAASIGKGAPLHKPKFSEALRTGLIFGAVETLTPLIGWGMGMLA
                     SRFVLEWNHWIAFVLLIFLGGRMIIEGFRGADDEDEEPRRRHGFWLLVTTAIATSLDA
                     MAVGVGLAFLQVNIIATALAIGCATLIMSTLGMMVGRFIGSIIGKKAEILGGLVLIGI
                     GVQILWTHFHG"
     gene            complement(514..1323)
                     /gene="rlmA"
                     /locus_tag="OO357_002933"
     CDS             complement(514..1323)
                     /gene="rlmA"
                     /locus_tag="OO357_002933"
                     /EC_number="2.1.1.187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (guanine(745)-N(1))-methyltransferase"
                     /protein_id="HCR5887805.1"
                     /translation="MSFSCPLCHQPLSREKNSYICPQRHQFDMAKEGYVNLLPVQHKR
                     SRDPGDSAEMMQARRAFLDAGHYQPLRDAIVAQLRERLDEKATAVLDIGCGEGYYTHA
                     FADALPEITTFGLDVSKVAIKAAAKRYPQVTLCVASSHRLPFSDTSMDAIIRIYAPCK
                     AEELARVVKPGGWVITATPGPRHLMELKGLIYNEVHLHAPHAEQLEGFTLQQSAELCY
                     PMRLRGDEAVALLQMTPFAWRAKPEVWQTLAAKEVFDCQTDFNIHLWQRSY"
     gene            complement(1489..1698)
                     /gene="cspE"
                     /locus_tag="OO357_002934"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
     CDS             complement(1489..1698)
                     /gene="cspE"
                     /locus_tag="OO357_002934"
                     /gene_synonym="gicA"
                     /gene_synonym="msmC"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator/RNA stability
                     regulator CspE"
                     /protein_id="HCR5887806.1"
                     /translation="MAKIKGQVKWFNESKGFGFITPADGSKDVFVHFSAIQGNGFKTL
                     AEGQNVEFEIQDGQKGPAAVNVTAI"
     gene            complement(1711..1854)
                     /locus_tag="OO357_002935"
     CDS             complement(1711..1854)
                     /locus_tag="OO357_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YobF family protein"
                     /protein_id="HCR5887807.1"
                     /translation="MCGIFSKEVLSKHVDVEYRFSAEPYIGASCSNVSVLSMLCLRAK
                     KTI"
     gene            complement(2525..2812)
                     /locus_tag="OO357_002936"
     CDS             complement(2525..2812)
                     /locus_tag="OO357_002936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebO family protein"
                     /protein_id="HCR5887808.1"
                     /translation="MNEVVNSGVMNIASLVVSVVVLLIGLILWFFINRASSRTNEQIE
                     LLEALLDQQKRQNALLRRLCEANEPEKADKKTIESQKSVDDEDIIRLVAER"
     gene            complement(2887..3030)
                     /gene="mgrB"
                     /locus_tag="OO357_002937"
     CDS             complement(2887..3030)
                     /gene="mgrB"
                     /locus_tag="OO357_002937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoP/PhoQ regulator MgrB"
                     /protein_id="HCR5887809.1"
                     /translation="MKKFRWVVLVVVVLACLLLWAQVFNMMCDQDVQFFSGICAINQF
                     IPW"
     gene            3189..3428
                     /locus_tag="OO357_002938"
     CDS             3189..3428
                     /locus_tag="OO357_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887810.1"
                     /translation="MRFIIRTVMLIALVWIGLLLSGYGVLIGSKENAAGLGLQCTYLT
                     ARGTSTVQYLHTKSGFLEITDCPLLRKSNIVVDNG"
     gene            complement(3571..4362)
                     /gene="kdgR"
                     /locus_tag="OO357_002939"
     CDS             complement(3571..4362)
                     /gene="kdgR"
                     /locus_tag="OO357_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator KdgR"
                     /protein_id="HCR5887811.1"
                     /translation="MANADLDKQPDSVSSVLKVFGILQALGEEREIGITELSQRVMMS
                     KSTVYRFLQTMKTLGYVAQEGESEKYSLTLKLFELGARALQNVDLIRSADIQMREISR
                     LTKETIHLGALDEDSIVYIHKIDSMYNLRMYSRIGRRNPLYSTAIGKVLLAWRDRDEV
                     KQILEGVEYKRSTERTITSTEALLPVLDQVREQGYGEDNEEQEEGLQCIAVPVFDRFG
                     VVIAGLSISFPTLRFSEERLQEYVAMLHTAARKISAQMGYHDYPF"
     gene            4539..5912
                     /locus_tag="OO357_002940"
     CDS             4539..5912
                     /locus_tag="OO357_002940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416342.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5887812.1"
                     /translation="MPKVQADGLPLPQRYGAILTIVIGISMAVLDGAIANVALPTIAT
                     DLHATPASSIWVVNAYQIAIVISLLSFSFLGDMFGYRRIYKCGLVVFLLSSLFCALSD
                     SLQMLTLARVIQGFGGAALMSVNTALIRLIYPQRFLGRGMGINSFIVAVSSAAGPTIA
                     AAILSIASWKWLFLINVPLGIIALLLAIRFLPPNGSRASKPRFDLPSAVMNALTFGLL
                     ITALSGFAQGQSLTLIAAELVVMVVVGIFFIRRQLSLPVPLLPVDLLRIPLFSLSICT
                     SVCSFCAQMLAMVSLPFYLQTVLGRSEVETGLLLTPWPLATMVMAPLAGYLIERVHAG
                     LLGALGLFIMATGLFSLVLLPASPADINIIWPMILCGAGFGLFQSPNNHTIITSAPRE
                     RSGGASGMLGTARLLGQSSGAALVALMLNQFGDNGTHVSLMAAAILAVIAACVSGLRI
                     TQPRARA"
     gene            complement(5958..6839)
                     /gene="htpX"
                     /locus_tag="OO357_002941"
     CDS             complement(5958..6839)
                     /gene="htpX"
                     /locus_tag="OO357_002941"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014229823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease HtpX"
                     /protein_id="HCR5887813.1"
                     /translation="MMRIALFLLTNLAVMVVFGLVLSLTGIQSSSVQGLMIMALLFGF
                     GGSFVSLLMSKWMALRSVGGEVIEQPRNERERWLVNTVATQARQAGIAMPQVAIYHAP
                     DINAFATGARRDASLVAVSTGLLQNMSPDEAEAVIAHEISHIANGDMVTMTLIQGVVN
                     TFVIFISRILAQLAAGFMGGNRDEGEESNGNPLIYFAVATVLELVFGILASIITMWFS
                     RHREFHADAGSAKLVGREKMIAALQRLKTSYEPQEATSMMAFCINGKSKSLSELFMTH
                     PPLDKRIEALRTGEYLK"
     gene            complement(7031..9079)
                     /gene="prc"
                     /locus_tag="OO357_002942"
     CDS             complement(7031..9079)
                     /gene="prc"
                     /locus_tag="OO357_002942"
                     /EC_number="3.4.21.102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy terminal-processing peptidase"
                     /protein_id="HCR5887814.1"
                     /translation="MNMFFRLTALAGLLAIAGQTFAVEDITRADQIPVLKEETQHATV
                     SERVTSRFTRSHYRQFDLDQAFSAKIFDRYLNLLDYSHNVLLASDVEQFAKKKTELGD
                     ELRSGKLDVFYDLYNLAQKRRFERYQYALSVLEKPMDFTGNDTYNLDRSKAPWPKNEA
                     ELNAQWDSKVKFDELSLKLTGKTDKEIRETLTRRYKFAIRRLAQTNSEDVFSLAMTAF
                     AREIDPHTNYLSPRNTEQFNTEMSLSLEGIGAVLQMDDDYTVINSMVAGGPAAKSKAI
                     SVGDKIVGVGQTGKPMVDVIGWRLDDVVALIKGPKGSKVRLEILPAGKGTKTRTVTLT
                     RERIRLEDRAVKMSVKTVGKEKVGVLDIPGFYVGLTDDVKVQLQKLEKQNVSSVIIDL
                     RSNGGGALTEAVSLSGLFIPAGPIVQVRDNNGKVREDSDTDGQVFYKGPLVVLVDRFS
                     ASASEIFAAAMQDYGRALVVGEPTFGKGTVQQYRSLNRIYDQMLRPEWPALGSVQYTI
                     QKFYRVNGGSTQRKGVTPDIIMPTGNEETETGEKFEDNALPWDSIDAATYVKSGDLTA
                     FEPELLKEHNARIAKDPEFQNIMKDIARFNAMKDKRNIVSLNYAVREKENNEDDATRL
                     ARLNERFKREGKPELKKLDDLSKDYQEPDPYLDETVNIALDLAKLEKARPAEQPAPVK
                     "
     gene            complement(9099..9797)
                     /gene="proQ"
                     /locus_tag="OO357_002943"
     CDS             complement(9099..9797)
                     /gene="proQ"
                     /locus_tag="OO357_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707288.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone ProQ"
                     /protein_id="HCR5887815.1"
                     /translation="MENQPKLNSSKEVIAFLAERFPHCFSAEGEARPLKIGIFQDLVD
                     RVAGEMNLSKTQLRSALRLYTSSWRYLYGVKPGATRVDLDGNPCGELDEQHVEHARKQ
                     LEEAKARVQAQRAEQQAKKREAAAAAGEKEDAPRRERKPRPTTPRRKEGAERKPRAQK
                     PVEKAPKTVKAPREEQHTPVSDISALTVGQALKVKAGQNAMDATVLEITKDGVRVQLN
                     SGMSLIVRAEHLVF"
     gene            complement(9894..10391)
                     /gene="msrC"
                     /locus_tag="OO357_002944"
     CDS             complement(9894..10391)
                     /gene="msrC"
                     /locus_tag="OO357_002944"
                     /EC_number="1.8.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine (R)-S-oxide reductase"
                     /protein_id="HCR5887816.1"
                     /translation="MNKTEFYADLNRDFNALMAGETSFLATLANTSALLYERLTDVNW
                     AGFYLLEDDTLVLGPFQGKIACVRIPVGRGVCGTAVARNQVQRIEDVHAFDGHIACDA
                     ASNSEIVLPLVVKNQIIGVLDIDSTVFARFTDEDEQGLRQLVAQLEKVLATTDYKKFF
                     ASVAG"
     gene            10521..11804
                     /gene="yebS"
                     /locus_tag="OO357_002945"
     CDS             10521..11804
                     /gene="yebS"
                     /locus_tag="OO357_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane integrity lipid transport subunit YebS"
                     /protein_id="HCR5887817.1"
                     /translation="MALNTPQITPTKKITVRAIGEELPRGDYQRCPQCDMLFSLPEIN
                     SHQSAYCPRCQAKIRDGRDWSLTRLAAMAFTMLLLMPFAWGEPLLHIWLLGIRIDANV
                     MQGIWQMTKQGDAITGSMVFFCVIGAPLILVSSIAYLWFGNRLGMNLRPVLLMLERLK
                     EWVMLDIYLVGIGVASIKVQDYAHIQAGVGLFSFVALVILTTVTLSHLNVEELWERYY
                     PQRPATRRDEKLRVCLGCHFTGYPDQRGLCPRCHIPLRLRRRHSLQKCWAALLASIVL
                     LLPANLLPISIIYLNGGRQEDTILSGIMSLASSNIAVAGIVFIASILVPFTKVIVMFT
                     LLLSIHFKCQQGLRTRILLLRMVTWIGRWSILDLFVISLTMSLINRDQILAFTMGPAA
                     FYFGAAVILTILAVEWLDSRLLWDAHESGNARFDD"
     gene            11773..14393
                     /gene="yebT"
                     /locus_tag="OO357_002946"
                     /pseudo
     CDS             11773..14393
                     /gene="yebT"
                     /locus_tag="OO357_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416348.2"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-binding membrane homeostasis protein YebT"
     gene            14473..15912
                     /gene="rsmF"
                     /locus_tag="OO357_002947"
                     /pseudo
     CDS             14473..15912
                     /gene="rsmF"
                     /locus_tag="OO357_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416349.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1407)-C(5))-methyltransferase
                     RsmF"
     gene            16030..16266
                     /locus_tag="OO357_002948"
     CDS             16030..16266
                     /locus_tag="OO357_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707284.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1480 family protein"
                     /protein_id="HCR5887818.1"
                     /translation="MKTSVRIGAFEIDDGELHGESPGDRTLTIPCKSDPDLCMQLDAW
                     DAETSIPALLNGEHSVLYRTRYDQQSDAWIMRLA"
     gene            16371..16562
                     /locus_tag="OO357_002949"
     CDS             16371..16562
                     /locus_tag="OO357_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416351.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebW family protein"
                     /protein_id="HCR5887819.1"
                     /translation="MFALVLFVCYLDGGCEDIVVDVYNTEQQCLYSMSDQRIRHGSCF
                     PIEDFIDGFWQPAQEYGDF"
BASE COUNT         3992 a         4204 c         4020 g         4348 t
ORIGIN      
        1 atttgctgca tcaatcggta aaggtgcccc cctccataaa ccgaaatttt ctgaagcatt
       61 gcgaaccggc cttatttttg gtgccgtcga aaccctgacg ccgctgatcg gctggggaat
      121 gggcatgtta gccagccggt ttgtccttga atggaatcac tggattgcgt ttgtgctgct
      181 gatattcctc ggcgggcgaa tgattattga gggttttcgt ggcgcagatg atgaagatga
      241 agagccgcgc cgtcggcacg gtttctggct actggtaacc accgcgattg ccaccagcct
      301 ggatgccatg gctgtgggtg ttggtcttgc tttccttcag gtcaacatta tcgcgaccgc
      361 attggccatt ggttgtgcaa ccttgattat gtcgacatta gggatgatgg ttggtcgctt
      421 tatcggctca attattggga aaaaagcgga aattctcggc gggctggtgc tgatcggcat
      481 cggcgtccag atcctctgga cgcacttcca cggttaataa gaacgctgcc agaggtgaat
      541 attaaagtcc gtctggcagt cgaacacttc ttttgctgcc agtgtttgcc agacttctgg
      601 cttcgcacgc caggcaaacg gcgtcatctg caataatgcg acggcttcat caccgcgaag
      661 acgcatcgga taacacaact ccgcactctg ctgtaatgta aaaccttcca gttgttctgc
      721 atgaggtgca tgaagatgta cttcattgta aatcagcccc ttcagctcca tcaaatgtcg
      781 cggtcccggc gtggcagtaa tgacccagcc gccgggcttc actactcgtg ctaattcttc
      841 tgctttacac ggcgcgtaaa tacgtattat ggcgtccata ctggtatcgg aaaacggcaa
      901 acggtggctg gaagcgacac aaagagtgac ctgcggatag cgtttcgccg ccgcttttat
      961 cgctaccttc gagacatcca ggccaaacgt ggtgatttcg ggcaacgcat cggcaaatgc
     1021 gtgtgtgtaa tacccttcac cacagccaat atccagcacc gccgtggcct tttcatcaag
     1081 ccgttcccgc agttgggcga caattgcatc acgcagcggc tgataatgtc cggcatctaa
     1141 gaatgcgcgg cgtgcttgca tcatttccgc gctgtcgccc ggatcacgag accgtttatg
     1201 ctgaacgggc agcagattga catacccttc tttcgccata tcaaactgat gtcgttgggg
     1261 acagatatag ctgttttttt cacgcgaaag aggctgatgg caaagtggac aggaaaacga
     1321 catgacaact ccggcaggat actaaaggcc gcaagtgtaa cgcgaatcac gcggcgggca
     1381 aatatccgaa cgccaggagc gaagataaag catagtaaaa agcctcgcat tcgacgaggc
     1441 tttatagcga gattgaagcg tattcacact tcagatcagt ggattcgatc agatagctgt
     1501 tacgttaaca gctgccggac ctttctggcc gtcctgaatt tcgaactcaa cgttctgacc
     1561 ttcagccaga gttttgaagc cattaccctg gatagcggag aagtgtacga acacatcttt
     1621 gctgccatca gccggagtaa tgaagccaaa acctttagac tcgttgaacc acttaacctg
     1681 acctttaatc tttgccattt gaaaaattcc ttagattgtt ttcttcgccc gcaggcataa
     1741 catagataaa actgacacat tactgcatga ggcaccaata taaggctcgg cagagaagcg
     1801 gtattcaacg tcaacgtgtt tactcaggac ttctttactg aaaatgccac acataaacag
     1861 aactgtacct cgtttaaccc gaaatctttt atcacataca acgttaatta tggcaagcca
     1921 tttttaaaca tatctcgatc agacgcacaa atctcgatac gtcttccact ttttttgcac
     1981 acttatgcaa cggaatacgc gccgatatat cattgtgctt aaccttgcca gttcaggcag
     2041 atacttaaac tggcgtagtt tctaacatag ttcaataagt tcagttgttc caggccagag
     2101 ggagaagtta ttacatagtg cgtgcaatat cacattttct gctatgcaat gaataaaaag
     2161 ttatatcact ttttctcata aaacagtcag ttaacggcta tcaattaccc taaagagaag
     2221 tcaatcccca aagggattgt aaatttaaaa taagaaaaat tgatgagtga gcaaaaaaaa
     2281 tcaagagaga aacgtttcgc taataaataa taccacgctt aatcacaaat ctgatgtaaa
     2341 catcctaata aattaatgag gaaaatatct ttttctgata tcccgttacg caaataaata
     2401 atatgataat tatttgaagt cagaccagag ttttaatttg aagcagttgc accatgacag
     2461 tgcgtataat caggatgaat tgagtaaccc ttcaacgacg gggttactca agattgagga
     2521 aggattatcg ttcggcgacc aggcgaataa tatcttcgtc atcaaccgat ttttgactct
     2581 caatggtctt tttatctgct ttctctggtt cgtttgcttc gcacaaacga cgtaacagtg
     2641 cattttgacg tttttgctga tccagcaacg cctcaagcag ttcaatctgt tcgttagtcc
     2701 gtgaactggc acgattgata aaaaaccaca agatgagccc gataagaaga accaccaccg
     2761 atacaaccaa agacgcaatg ttcatcacgc ctgaatttac aacttcgttc atttcaccac
     2821 ctctgagtag aggcgctatt ctaccactgc tggagaggaa gaaaatctag tgctgaaaaa
     2881 atgatatcac cacgggataa actggttaat ggcacaaatt ccgctgaaaa attgtacatc
     2941 ctgatcgcac atcatgttga atacctgcgc ccaaagcagc aagcaagcca acaccacgac
     3001 aaccagaacg acccatcgaa actttttcac tccaatctcc gcttcaccta ccttatgtca
     3061 ttaaactagc atgcgttagt taaacagcgc ctaactatgt cgatattcgt gctttttcgg
     3121 aatgagtagc ttgcttcagc gcatattgcc gctacgatta agcgaacata aaaagagaag
     3181 agattgtaat gcgattcatc attcgcacag ttatgctgat agcactggta tggataggat
     3241 tattactcag cggatatggc gttttgattg gaagcaaaga gaatgccgca ggattaggtt
     3301 tgcaatgtac ctatctgact gccagaggca ccagcacggt acaatacttg cacactaaaa
     3361 gtggtttctt ggagataaca gattgcccac tgttacgtaa aagcaatatc gtggtcgata
     3421 atggttaagg gtaataaata accagattct cactcatgag taaaatattc ttatagcaag
     3481 cgcgtttaat ataacgcacc ttcaacttta tttttctcat gctaaataaa gaacctgtct
     3541 gtttgatatt ccgacaggtt cttactctca tcagaacgga tagtcgtgat aacccatttg
     3601 ggcagaaatt ttgcgcgctg cggtatgcaa cattgcgaca tattcctgta aacgctcttc
     3661 agagaaacgc aacgtcggga aggagatgct caaaccggca atgaccacgc caaagcgatc
     3721 aaataccggt accgcaatgc attgcagccc ttcttcctgc tcttcgttat cttctccata
     3781 cccctgctcg cgcacctggt ccagaacggg taataacgct tctgtactgg tgatggtccg
     3841 ctcggtactg cgtttatact ccacgccctc aagaatttgc ttcacttcat cgcgatcgcg
     3901 ccatgccagc agtaccttac caatcgcggt gctgtacagc ggattacgac gcccaatccg
     3961 tgaatacatg cgcaaattgt acatagagtc aattttgtga atgtaaacaa tactgtcttc
     4021 gtccagtgcg ccgaggtgga tagtttcttt ggtcaggcgg gagatctcac gcatctggat
     4081 atctgcgcta cgaattaaat cgacgttttg taacgcgcga gcgcccagtt caaacaattt
     4141 cagggtcagg gaatatttct ccgactcccc ttcctgcgcc acataaccta aggttttcat
     4201 ggtctgtaaa aagcgataaa cggtgctttt tgacatcatg acgcgctgcg acagctcggt
     4261 tatccctatt tcgcgctctt cacccagcgc ctgcaaaatg ccaaaaactt ttagcacgga
     4321 agatacagaa tcaggctgtt tatccagatc tgcgttagcc atttatcacc tcattgcgag
     4381 tgttttataa aaatcagaac tgttttttat tataatttcg caccagggtg gtcgcaatcc
     4441 atcttttgcc ggttagttac aattctgcga catccaccgt gaatatcagt gctagaatca
     4501 tacccctgtt gattattcac caaagatata aaattcctat gccaaaagtt caggccgacg
     4561 gcctgccatt gccccagcga tacggtgcga tattaaccat tgtgattggt atttcgatgg
     4621 ctgtccttga cggcgcaatc gccaacgtcg ccctgccaac aattgccacg gaccttcatg
     4681 ccacaccagc cagttccatc tgggtagtga acgcctatca aatcgccatt gtcatctccc
     4741 tgctctcgtt ttcgtttctg ggcgatatgt ttggctatcg acgtatttat aaatgcggtc
     4801 tggtcgtttt tctgttgtct tcactgttct gcgccctttc tgattcgctg caaatgctca
     4861 cccttgcgcg tgtcatacaa ggtttcggcg gtgcagcgtt gatgagcgtt aataccgcac
     4921 ttatccgcct gatctatcca caacgttttc tggggagagg gatgggcata aactcgttta
     4981 ttgttgccgt ctcttctgct gccgggccga caattgctgc agcaatcctc tccatcgcat
     5041 cctggaaatg gttattttta atcaacgtac cgttaggtat tatcgccctg cttctggcga
     5101 tacgttttct gccacccaat ggttctcgcg ccagtaaacc ccgtttcgac ctgcccagcg
     5161 ccgtgatgaa cgcgttaacc ttcggcctgc ttatcactgc gttgagtggt ttcgctcagg
     5221 ggcaatcgct gacgttaatt gctgcggaac tggtggtaat ggttgttgtt ggtattttct
     5281 ttattcgccg ccagctttct cttcccgtac cgctgctacc ggtggattta ctgcgtatcc
     5341 cgctgttttc actttctatt tgcacatctg tttgctcttt ctgcgcacaa atgctggcaa
     5401 tggtttccct gcccttttac ctgcaaaccg tgctcgggcg tagtgaagtc gaaacaggtt
     5461 tacttctgac accgtggccg ttagcaacga tggtgatggc tcctctggca ggctatttga
     5521 ttgaacgcgt acatgcagga ttgctgggtg ctttagggtt gttcatcatg gctacggggc
     5581 ttttttccct ggttctgctg cccgcgtcac ctgcggatat caatattatc tggccgatga
     5641 tcttatgtgg cgctggattt ggcttattcc agtcacccaa taaccacacc attattacct
     5701 ccgctccgcg cgaacgtagc ggtggggcca gtggcatgtt aggaacggct cgtctactgg
     5761 gtcagagtag tggcgcggcg ctggtggcgc tgatgctaaa tcagtttggt gataatggta
     5821 cgcacgtctc gctgatggct gcggctattc tggcagtgat tgctgcctgt gtcagtggtt
     5881 tacgtatcac tcagccacga gccagggcat aaaaaaagcg cgtcgatcag gacgcgcttt
     5941 ttagtattta cttcatatta cttcaggtat tcacccgtac gcagagcttc aatacgttta
     6001 tccagcggcg ggtgagtcat gaacaactca ctgagcgatt tcgacttacc gttaatgcag
     6061 aaagccatca tgctggttgc ttcttgcggt tcatagctgg ttttcaggcg ctgcagcgcg
     6121 gcaatcattt tctcgcgacc aaccagtttt gccgaaccgg catctgcatg gaattcacga
     6181 tgacgcgaga accacatggt gataatgctc gccagaatac caaacaccag ttccagaacc
     6241 gttgcaaccg caaagtagat cagcgggttg ccgttgctct cttcaccttc atcacgattt
     6301 ccgcccataa aacccgcggc aagctgcgcc agaatacggg aaataaagat aacgaaggtg
     6361 ttcaccacgc cctgaatcag cgtcatggtg accatatcac cattggcgat gtggctgatt
     6421 tcgtgagcaa ttaccgcctc ggcttcatcc gggctcatgt tctgcagcaa accggtgctg
     6481 acagcaacca gagaggcatc acggcgcgca ccggttgcaa aagcgttgat gtccggcgca
     6541 tggtagatag ccacttgcgg catagcgatc cccgcctgac gagcctgggt tgctacagta
     6601 ttgaccagcc aacgttccct ttcgttacgc ggttgctcga tcacttcccc gccaacagat
     6661 cgtaatgcca tccatttgga catcagaagc gaaacgaagg aaccaccaaa accgaacagc
     6721 aaggccatga tcatcagccc ctgaacgctg ctcgactgta tccctgtcag gctcagtacc
     6781 agcccgaaaa cgaccattac ggccaggttc gttagcagga agagcgcgat tcgcatcata
     6841 attttctttt tacctcagtt taacaaaacg caatatgcga tacccacatc gtatgggtta
     6901 cgcgactatt ttcaagtctg gatagtgcgt aagtcaccag aaagacacaa ctttacattt
     6961 tgtagcatct gatttacggc atcttgccgc tgttaaaaaa tcaggcacaa tttcttgtgc
     7021 ctgattgata ttacttgacg ggagcgggtt gttccgcggg tctggctttt tcaagcttcg
     7081 ccagatcgag tgcgatattc accgtctcat ccagataagg atccggctcc tggtaatctt
     7141 tcgatagatc atccagcttc ttcaactccg gtttaccttc tcgtttaaag cgttcgttca
     7201 aacgcgccag acgcgtcgca tcatcttcat tattctcttt ctcacgcaca gcgtagttca
     7261 gagaaacgat attgcgcttg tccttcatag cgttgaagcg cgcgatatcc ttcatgatgt
     7321 tctggaactc aggatctttc gcgatacgcg cattatgttc cttcagcagc tccggttcaa
     7381 aggcagttaa atctcctgat ttcacataag tcgcggcatc gatgctatcc cacggcagcg
     7441 cgttatcttc gaatttctca cccgtttccg tttcttcatt acccgtcggc atgatgatgt
     7501 ctggtgttac gcctttacgt tgcgtactgc cgccgttaac gcgatagaat ttctggatcg
     7561 tgtactgcac agaacccagc gctggccatt caggacgtaa catctgatcg taaatacggt
     7621 tcaatgaacg gtattgctga acggtgcctt taccaaacgt cggttcaccc acaaccagcg
     7681 cacgaccgta atcctgcatt gccgcggcaa agatttctga agccgaagca ctgaaacggt
     7741 caaccagtac caccagcggg cctttataga acacctgccc gtcggtatcg ctgtcttcac
     7801 gaaccttgcc gttgttatcg cggacctgaa caatgggacc cgcaggaata aacagacctg
     7861 agagcgatac cgcttcggtc aacgccccac cgccattgct acgcaggtcg atgatcacac
     7921 tgctgacatt ctgtttttcc agtttctgca gttgaacttt gacatcgtct gtcaaaccca
     7981 catagaagcc cggaatatcc agcacgccga ctttctcttt accgacggtc ttcaccgaca
     8041 ttttaaccgc gcggtcttcg agacgaatac gttcacgggt caacgttaca gtacgggtct
     8101 tggtcccttt accagcaggt aaaatttcca gacgaacttt actgcccttc ggccctttaa
     8161 ttaaggcaac cacatcatca agacgccagc caatcacgtc aaccatcggc ttgcctgttt
     8221 gaccaacacc gacaattttg tcaccaacgc tgatagcttt actcttcgct gccggaccac
     8281 ctgccaccat cgaattgata acggtgtagt catcatccat ttgcagcact gcgccaatac
     8341 cttccagtga caaactcatt tcagtgttga actgttcggt attacgcggg gaaagatagt
     8401 tggtatgcgg atcgatttca cgcgcaaacg ccgtcattgc cagcgagaaa acatcttcgc
     8461 tgttggtttg cgccagacga cgaatggcaa atttgtagcg gcgtgtcagg gtttcacgaa
     8521 tttctttatc cgtttttcct gtcagcttca ggcttaactc gtcgaatttg actttactgt
     8581 cccactgcgc gttcaactca gcctcgtttt tcggccaggg cgctttgctg cggtcaaggt
     8641 tataagtgtc gttgccggtg aaatccatcg gcttttccag taccgacaaa gcgtactggt
     8701 aacgttcaaa acggcgcttt tgcgccagat tgtagagatc gtagaaaacg tcgagtttgc
     8761 ctgaacgcag ttcatcgcct aactcggttt tctttttcgc gaactgttca acatcgcttg
     8821 ccagcagcac attgtggctg taatcgagca gattcaggta gcggtcaaag attttggccg
     8881 aaaatgcctg atcgaggtcg aactggcgat aatgagaacg ggtgaagcgc gacgttacgc
     8941 gctcacttac cgtcgcatgc tgcgtctctt cctttaatac cggaatttga tcagcacgcg
     9001 tgatatcttc tacagcgaag gtctggcctg ctattgcaag caggccagct aacgcggtaa
     9061 gcctaaaaaa catgttcatg cctggcccgg cctccgtttc agaacaccag gtgttctgcg
     9121 cgcacaatca aagacatacc cgaattcagc tggacgcgga cgccgtcttt ggtgatttct
     9181 aatacggtgg catccatcgc gttttgaccc gctttcacct tcagggcttg tccgacagtc
     9241 agagctgaaa tgtcagaaac cggggtgtgc tgttcttcgc gaggtgcttt tactgttttt
     9301 ggcgctttct ctaccggctt ttgcgcacga ggtttacgtt cagcgccttc tttgcggcgt
     9361 ggcgtggtcg gacgtggctt gcgttcgcgg cgcggtgcgt cttctttctc accagcagct
     9421 gcggcagctt cgcgtttttt cgcttgctgt tcagcacgct gtgcctgaac acgcgctttc
     9481 gcttcttcaa gctgcttgcg agcatgctct acatgttgct cgtccagctc accgcatggg
     9541 ttgccgtcaa gatcgacacg cgttgcgccg ggtttaacac cgtaaagata acgccagctc
     9601 gaagtgtaga gacgtaaagc ggatcgtaat tgcgttttgc tcaggttcat ttccccggca
     9661 acacgatcga ccaaatcctg aaaaatacca attttcagcg gacgcgcttc accttccgca
     9721 ctgaaacagt ggggaaaacg ttcggccaga aacgcgatta cttctttact gctattcaac
     9781 ttaggttgat tttccatgaa atttcctgat tacaacggac gtagccaaca agcgcaggca
     9841 tgaacaggcg tcattataat gacgctacca gtaaatgcta cgttatccgt tgattatcct
     9901 gcgacgctcg caaagaattt tttgtaatcc gtcgttgcaa gcactttttc aagctgtgcc
     9961 acaagctgac gtaagccttg ctcgtcctcg tctgtaaagc gagcgaagac agtactatcg
    10021 atgtcgagaa caccaataat ctgatttttc accaccagcg gcagaacaat ttcagaatta
    10081 ctcgccgcat cacaggcaat atgcccgtca aacgcgtgaa catcctcgat acgctgcact
    10141 tgattgcggg caaccgctgt gccgcacacc ccgcgcccga cgggtatccg gacacaggca
    10201 attttgccct gaaatggtcc gagtaccagt gtatcgtcct caagcaaata aaaacctgcc
    10261 cagttaacgt cagtgagacg ctcgtataac aacgcactgg tgttcgcaag cgttgccaga
    10321 aaactggttt ctcccgccat cagcgcgtta aagtcgcgat ttaaatccgc gtaaaattct
    10381 gttttgttca ttacttaatc acttggttgt cttacctgga actgccagcc tattaaaata
    10441 agcattaaat gcgttaatgc tcaagatcat tcccatcatg ggttaagatt aatgttaatt
    10501 cttattacat ttggcacgtc atggctctta acacaccaca aattacgccg acaaaaaaga
    10561 taacagtgag ggcaatcggc gaggaactgc cgcgtggtga ttaccaacgt tgcccgcaat
    10621 gtgacatgct gtttagcctg cccgagataa attctcatca aagtgcctat tgtccgcgct
    10681 gtcaggcaaa aattcgcgac gggcgcgact ggtcgctaac gcgcctggcg gcaatggcct
    10741 tcaccatgct gttgcttatg ccgtttgcct ggggcgaacc gctgttgcat atctggctgt
    10801 taggcattcg tattgacgcc aacgtcatgc aaggcatctg gcaaatgacc aaacagggcg
    10861 atgcgataac gggatcaatg gtctttttct gcgttattgg tgcccccctc attctggtgt
    10921 cctccatagc atatttatgg tttggtaacc gactgggaat gaatttacgt ccggtactgc
    10981 tgatgcttga gcgacttaaa gagtgggtaa tgctggatat ctacctggtc ggcattggcg
    11041 ttgcttctat aaaggtacag gattatgccc atatccaggc gggtgtcggc ttgttctctt
    11101 ttgtggcgtt ggtgatttta acgacggtga cgttgtcaca tcttaatgtt gaggagctgt
    11161 gggagcgata ttatccgcag cgccccgcta cgcgtaggga cgagaaactt cgtgtctgtc
    11221 ttgggtgcca ttttaccggc tatcctgatc agcgtggtct ctgcccgcgt tgccatatcc
    11281 cgctacgcct gcgtcgtcgt catagtttgc aaaaatgctg ggcggcgctg ttagcgtcaa
    11341 tcgttttgtt gttaccagcc aacctattgc ctatttctat catttatctg aatggtggcc
    11401 ggcaggaaga tacgattctt tccggaatta tgtcgctggc aagtagcaac attgcggttg
    11461 cgggaatcgt gtttatcgcc agtattctgg taccgtttac taaagtgatc gtcatgttca
    11521 ctttactgtt gagcattcat tttaaatgcc agcaaggttt acgcacacgc attctgttac
    11581 tgcggatggt gacctggatt ggacgctggt cgatactcga cctgtttgtc atatctttaa
    11641 ccatgtcgct gattaatcgc gatcagatcc tcgcttttac tatgggaccg gctgcgtttt
    11701 attttggcgc tgccgtaatt ttgactattc ttgctgtgga atggctggac agccgcttac
    11761 tttgggatgc acatgagtca ggaaacgccc gcttcgacga ctgaagcgca gattaaaaat
    11821 aaacgccgta tctcaccttt ctggctgctg cctttcatcg cgctaatgat tgccggttgg
    11881 ctgatttggg acagttatca ggaccggggt aataccgtca ccatcgactt tatgtcggcg
    11941 gatggtattg ttccgggccg tacgcctgtt cgttatcagg gcgttgaagt cggaacagtg
    12001 caggatatca gcctcagcga cgatcttcgt aagattgaag tcaaggtcag catcaagtcc
    12061 gatatgaaag atgcgctgcg cgaagagact cagttctggc tgatgacgcc aaaagcatcg
    12121 ttggcaggtg tctccgggct ggacgccctc gtcggtggga actatatcgg catgatgccg
    12181 ggtaaaggta aagagcagga tcactttgtc gcgctcgata cccaaccgaa atatcggctg
    12241 gacaatggcg atctgatgat ccacctgcaa gcccccgctc tcggttcgct gaacagcggt
    12301 tcattggtct atttccgcaa gatcccggtg ggaaaagtct acgactatgc catcaatccc
    12361 aacaagcaag gcgtggtgat tgatgtcctg atcgagcggc gttttaccga tctggtgaaa
    12421 aaaggtagcc gtttctgaaa cgtttccggc gttgatgcca acgtcagtat cagtggcgcg
    12481 aaggtgaaac tggaaagtct ggcggcactg gttaacggtg cgattgcctt cgattcacct
    12541 gaagagtcga aacctgccga ggcggaagat acctttggtc tgtatgaaga tctggcccac
    12601 agccagcgtg gagtaataat aaaactggaa ctgccgagtg gagcaggatt aaccgccgac
    12661 tcgacgccgt taatgtatca ggggctggaa gtcggacagc tgactaaact ggatttaaat
    12721 cctggtggta aagtcactgg ggaaatgacc gttgatccct gcttcgtgaa aatacccgca
    12781 tcgaattacg caacccgaaa ttatccctta gcgatgctaa tctcagcgcc ctgctgaccg
    12841 gaaaaacctt tgagttggtg cccggcgatg gcgagccacg caaagagttc gttgttgtgc
    12901 cgggcgaaaa agccctgctg caagagccgg acgttctgac actgaccctg acagcaccgg
    12961 aaagttacgg tattgatgcg ggtcagccgc tcattcttca cggcgtgcag gtaggccagg
    13021 ttatcgatcg taaactcacc agcaaaggcg tcacctttac cgtcgccatc gagcctcagc
    13081 atcgagaact ggtaaaaggc gatagcaaat ttgtcgtcaa cagccgtgtc gacgtgaagg
    13141 tggggctgga tggcgttgag tttctcggtg ccagcgcctc agaatggatt aacggcggga
    13201 tacgtattct gccgggcgat aaaggcgaga tgaaagccag ctatccactg tatgccaatc
    13261 tggaaaaagc gctggagaac agccttagcg atttacccac cacaaccatg agtttgagtg
    13321 cagagacgct gccggatgtg caggcaggat cggtagtgct gtaccgtaaa tttgaagttg
    13381 gtgaagttat taccgtgcgt ccgcgagcta acgcgttcga tatcgatctg cacattaaac
    13441 cggaatatcg caaccttttg accagcaaca gcgtgttctg ggcagaaggc ggggcgaaag
    13501 ttcagctgaa tggtagtggc ctgaccgtac aggcatcccc gctctccaga gcattaaagg
    13561 gggccattag cttcgataac ctcagtggtg ccagcgccag tcagcgtaaa ggcgacaagc
    13621 gaattctgta tgcttccgaa acagcggccc gtgcggttgg caggcagatt acgcttcacg
    13681 ctttcgatgc cggaaaactg gcggtcggga tgccaattcg ctatctcggt attgatatcg
    13741 ggcaaatcca gacgctggat ctgattaccg cacgcaatga agtacaggca aaggcggtgc
    13801 tctatccgga atatgtccag acctttgctc gcggtggtac gcgcttctca gtggtcacac
    13861 cgcaaatctc ggcagctggc gttgagcatc tagatactat cctccagccg tatatcaacg
    13921 tcgaaccagg ccgtggcaat cctcgtcgcg actttgaatt agaagaggcc accattactg
    13981 attcgcgtta cctagatggt ttaagcatta ttgttgaagc gccggaagcc ggttcgttag
    14041 gcatcggtac gcctgtgctg ttccgtggtc tggaagtagg tacggttacc ggaatgacgc
    14101 tggggacatt gtcagatcgc gtgatgattg cgatgcgcat cagtaaacgc tatcaacacc
    14161 tggtgcgtaa caattccgtc ttctggttgg catcgggtta cagtctggac tttggtctga
    14221 cgggcggcgt agtgaaaacc ggcaccttta accaatttat ccgtggcggc atcgccttcg
    14281 ccacgcctcc gggtacgcca cttgcaccga aagcccaaga aggcaaacac ttcctgttgc
    14341 aggaaagtga accgaaagag tggcgtgaat ggggaactgc gcttcccaaa taatgcccac
    14401 tgctccggcg tgcctgcgcc ggagcgctta tgctaaactg cgcgcctgtt tttttgccag
    14461 tggtacatgc tcgtggccca acacaccgtt tatttcccgg acgcgtttct gacacaaatg
    14521 cgcgaagcta tgccttcgac gctctcattt gatgattttc ttgccgcctg tcagcgcccg
    14581 ttgcgccgca gcattcgcgt taatacgctg aaaatctccg ttgctgattt cctgcaatta
    14641 accgctcctt atggctggac gcttacgcca attccgtggt gtgaagaagg tttctggatt
    14701 gaacgcgaca atgaagatgc attgccattg ggtagtacca ccgagcattt aagtggcctg
    14761 ttttatattc aggaagccag ttcaatgttg cccgtcgccg ccttgtttgc tgacggtaat
    14821 gcaccacagc gggtgatgga tgtcgctgct gcgccaggct caaaaacgac gcaaattgcc
    14881 gcgcggatga ataacgaagg ggcaatcctt gccaatgagt tttccgccag ttgggtaaaa
    14941 gtgttacatg ccaatatcag ccgctgtggc atcagtaatg ttgcgctcac acattttggt
    15001 ggccgcgtgt ttggtgcggc agtgccagaa atgtttgatg ccattttgct ggacgctccc
    15061 tgctccggcg aaggcgtggt gcgtaaagat cccgatgcgc taaaaaactg gtcacccgaa
    15121 agcaatcagg aaatcgcagc tacccaacgg gaactgattg acagcgcctt tcatgcatta
    15181 cgtcctggcg gtacgctggt ttactcgacc tataccttaa accgggaaga aaacgaagcc
    15241 gtttgccggt ggctgaaaga gacttacccc gacgaagtag agtttttacc acttggcgat
    15301 ctcttccctg gtgcaaacaa ggcgctgacc gaagaaggtt ttttgcatgt tttcccacaa
    15361 atttacgact gcgaaggctt cttcgttgct cgtctgcgta aaactcaggc gatccccgcc
    15421 ttacccgccc caaaatacaa agtgggcaat ttcccgttca gcccggtgaa agatcgcgaa
    15481 gccggacaaa ttcgtcaggc ggctgcaagt gttggcttaa actgggatgg aaacctgcga
    15541 ctctggcaac gcgacaaaga actgtggttg ttcccggtag gcattgaagc cctgatcggt
    15601 aaagtccgat tttctcgctt agggattaaa cttgccgaaa cgcataacaa aggttatcgc
    15661 tggcagcatg aagcggttat tgcccatgcc tcccccgaca atgtaaacgc ttttgaactg
    15721 acaccgcagg aagcgtagga gtggtatcgc gggcgcgatg tttacccgca agccgcgcca
    15781 gtggcggatg acgtgttggt tactttccag catcagccga ttggtttagc caaacggatt
    15841 ggttcgcgac tgaaaaacag ctacccgcgt gaactggtgc gggacgggaa actttttacc
    15901 agtaacgcat gacagcgcac aaaaaaagcg cactttttga ctggcacatt cgccggcctc
    15961 aactaggctg aaaaattgtg cgatcggact ggtcgtacta caatcagcag ctaaatggag
    16021 agcacgaaga tgaaaaccag tgtgcgcata ggcgcttttg aaatcgacga cggcgaatta
    16081 cacggtgaat cgccgggtga tcgaacgtta accattcctt gtaaatctga ccccgattta
    16141 tgtatgcaac tggatgcctg ggatgctgaa accagtatcc ctgccctgct aaatggcgaa
    16201 cactctgtcc tttaccgtac ccgttacgat caacaatctg atgcctggat tatgcgtctt
    16261 gcctgatcca aaaagaaccc gtcggcatgg cgggttattt gtcctggtta ttcccccgtt
    16321 gtaaaatctc tcctaaactt aacggtacgg caccacactt cggggatgaa atgttcgcgc
    16381 tggtactttt tgtttgctac ctggatggcg gttgtgaaga tattgttgtg gatgtctaca
    16441 acacggaaca gcagtgtctt tattctatga gcgatcaacg gatccgccat ggcagttgtt
    16501 ttccgattga ggattttata gatggtttct ggcaaccagc acaggagtat ggtgattttt
    16561 aatt
//
LOCUS       DAJTRC010000082        16466 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.082, whole genome shotgun sequence.
ACCESSION   DAJTRC010000082 DAJTRC010000000
VERSION     DAJTRC010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16466)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16466)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16466
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.082"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(33..2474)
                     /gene="rnr"
                     /locus_tag="OO357_002950"
     CDS             complement(33..2474)
                     /gene="rnr"
                     /locus_tag="OO357_002950"
                     /EC_number="3.1.13.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HCR5887820.1"
                     /translation="MSQDPFQEREAEKYANPIPSREFILEHLTKREKPASRDELAVEL
                     HIEGEEQLEGLRRRLRAMERDGQLVFTRRQCYALPERLDLVKGTVIGHRDGYGFLRVE
                     GRKDDLYLSSEQMKTCIHGDQVLAQPLGADRKGRREARIVRVLVPKTSQIVGRYFTEA
                     GVGFVVPDDSRLSFDILIPPDQIMGARMGFVVVVELTQRPTRRTKAVGKIVEVLGDNM
                     GTGMAVDIALRTHEIPYIWPQAVEQQVAGLKEEVPEEAKAGRVDLRDLPLVTIDGEDA
                     RDFDDAVYCEKKRGGGWRLWVAIADVSYYVRPPTPLDREARNRGTSVYFPSQVIPMLP
                     EVLSNGLCSLNPQVDRLCMVCEMTVSSKGRLTGYKFYEAVMSSHARLTYTKVWHILQG
                     DQDLREQYAPLVKHLEELHNLYKVLDKAREERGGISFESEEAKFIFNAERRIERIEQT
                     QRNDAHKLIEECMILANISAARFVEKAKEPALFRIHDKPSTEAITSFRSVLAELGLEL
                     PGGNKPEPRDYAELLESVADRPDAEMLQTMLLRSMKQAIYDPENRGHFGLALQSYAHF
                     TSPIRRYPDLTLHRAIKYLLAKEQGHQGNTTETGGYHYSMEEMLQLGQHCSMAERRAD
                     EATRDVADWLKCDFMLDQVGNVFKGVISSVTGFGFFVRLDDLFIDGLVHVSSLDNDYY
                     RFDQVGQRLMGESSGQTYRLGDRVEVRVEAVNMDERKIDFSLISSERAPRNVGKTARE
                     KAKKGDAGKKGGKRCQVGKKVNFEPDSAFRGEKKTKPKAAKKDARKAKKPSAKTQKIA
                     AATKAKRAAKKKVAE"
     gene            complement(2513..2938)
                     /gene="nsrR"
                     /locus_tag="OO357_002951"
     CDS             complement(2513..2938)
                     /gene="nsrR"
                     /locus_tag="OO357_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitric oxide-sensing transcriptional repressor
                     NsrR"
                     /protein_id="HCR5887821.1"
                     /translation="MQLTSFTDYGLRALIYMASLPEGRMTSISEVTDVYGVSRNHMVK
                     IINQLSRAGYVTAVRGKNGGIRLGKPASAIRIGDVVRELEPLSLVNCSSEFCHITPAC
                     RLKQALSKAVQSFLTELDNYTLADLVEENQPLYKLLLVE"
     gene            complement(3143..4441)
                     /gene="purA"
                     /locus_tag="OO357_002952"
     CDS             complement(3143..4441)
                     /gene="purA"
                     /locus_tag="OO357_002952"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HCR5887822.1"
                     /translation="MGNNVVVLGTQWGDEGKGKIVDLLTERAKYVVRYQGGHNAGHTL
                     VINGEKTVLHLIPSGILRENVTSIIGNGVVLSPAALMKEMKELEDRGIPVRERLLLSE
                     ACPLILDYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVGDLFDKETFAE
                     KLKEVMEYHNFQLVNYYKAEAVDYQKVLDDTMAVADILTSMVVDVSDLLDQARQRGDF
                     VMFEGAQGTLLDIDHGTYPYVTSSNTTAGGVATGSGLGPRYVDYVLGILKAYSTRVGA
                     GPFPAELFDETGEFLCKQGNEFGATTGRRRRTGWLDTVAVRRAVQLNSLSGFCLTKLD
                     VLDGLKEVKLCVAYRMPDGREVTTTPLAADDWKGVEPIYETMPGWSESTFGVKDRSGL
                     PQAALNYIKRIEELTGVPIDIISTGPDRTETMILRDPFDA"
     gene            complement(4545..4742)
                     /locus_tag="OO357_002953"
     CDS             complement(4545..4742)
                     /locus_tag="OO357_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HCR5887823.1"
                     /translation="MNSTIWLALALVLVLEGLGPMLYPKAWKKMISAMTNLPDNILRR
                     FGGGLVVAGVVVYYMLRKTIG"
     gene            complement(4824..5828)
                     /gene="hflC"
                     /locus_tag="OO357_002954"
     CDS             complement(4824..5828)
                     /gene="hflC"
                     /locus_tag="OO357_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HCR5887824.1"
                     /translation="MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDDNK
                     PLVYEPGLHFKIPFIETVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSR
                     YYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSA
                     GTEDEVTTPAADNAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTE
                     VSEAIYNRMRAEREAVARRHRSQGQEEAEKLRATADYEVTRTLAEAERQGRIMRGEGD
                     AEAAKLFADAFSKDPDFYAFIRSLRAYENSFSGNQDVMVMSPDSDFFRYMKTPTSATR
                     "
     gene            complement(5831..7090)
                     /gene="hflK"
                     /locus_tag="OO357_002955"
     CDS             complement(5831..7090)
                     /gene="hflK"
                     /locus_tag="OO357_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HCR5887825.1"
                     /translation="MAWNQPGNNGQDRDPWGSSKPGGNSEGNGNKGGRDQGPPDLDDI
                     FRKLSKKLGGLGGGKGTGSGGGSSSQGPRPQLGGRVVTIAAAAIVIIWAASGFYTIKE
                     AERGVVTRFGKFSHLVEPGLNWKPTFIDEVKPVNVEAVRELAASGVMLTSDENVVRVE
                     MNVQYRVTNPEKYLYSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQR
                     ELEETIRPYDMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYSNEVQ
                     PRANGQAQRILEEARAYKAQTILEAQGEVARFAKLLPEYKAAPEITRERLYIETMEKV
                     LGNTRKVLVNDKGGNLMVLPLDQMLKGGNAPAAKSDNGASNLLRLPPASSSTTSGASN
                     TSSTSQGDIMDQRRANAQRNDYQRQGE"
     gene            complement(7176..8456)
                     /gene="hflX"
                     /locus_tag="OO357_002956"
     CDS             complement(7176..8456)
                     /gene="hflX"
                     /locus_tag="OO357_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HCR5887826.1"
                     /translation="MFDRYDAGEQAVLVHIYFTQDKDMEDLQEFESLVSSAGVEALQV
                     ITGSRKAPHPKYFVGEGKAVEIAEAVKAMGASVVLFDHALSPAQERNLERLCECRVID
                     RTGLILDIFAQRARTHEGKLQVELAQLRHLATRLVRGWTHLERQKGGIGLRGPGETQL
                     ETDRRLLRNRIVQIQSRLERVEKQREQGRQSRIKADVPTVSLVGYTNAGKSTLFNRIT
                     EARVYAADQLFATLDPTLRRIDVADVGETVLADTVGFIRHLPHDLVAAFKATLQETRQ
                     ATLLLHVIDAADVRVQENIEAVNTVLEEIDAHEIPTLLVMNKIDMLEDFEPRIDRDEE
                     NKPIRVWLSAQTGAGIPQLFQALTERLSGEVAQHTLRLPPQEGRLRSRFYQLQAIEKE
                     WMEEDGSVSLQVRMPIVDWRRLCKQEPALIDYLI"
     gene            complement(8532..8840)
                     /gene="hfq"
                     /locus_tag="OO357_002957"
     CDS             complement(8532..8840)
                     /gene="hfq"
                     /locus_tag="OO357_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA chaperone Hfq"
                     /protein_id="HCR5887827.1"
                     /translation="MAKGQSLQDPFLNALRRERVPVSIYLVNGIKLQGQIESFDQFVI
                     LLKNTVSQMVYKHAISTVVPSRPVSHHSNNAGGGTSSNYHHGSSAQNTSAQQDSEETE
                     "
     gene            complement(8926..9876)
                     /gene="miaA"
                     /locus_tag="OO357_002958"
     CDS             complement(8926..9876)
                     /gene="miaA"
                     /locus_tag="OO357_002958"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HCR5887828.1"
                     /translation="MSDISKASLPKAIFLMGPTASGKTALAIELRKILPVELISVDSV
                     LIYKGMDIGTAKPNAEELLAAPHRLLDIRDPSQAYSAADFRRDALAEMADITAAGRIP
                     LLVGGTMLYFKALLEGLSPLPSADPEVRARIEQQAAEQGWESLHRQLQEVDPVAAARI
                     HPNDPQRLSRALEVFFISGKTLTELTQTSGDALPYQVHQFAIAPASRELLHQRIEQRF
                     HQMLASGFEAEVRALFARGDLHTDLPSIRCVGYRQMWSYLEGEISYDEMVYRGVCATR
                     QLAKRQITWLRGWEGVHWLDSEKPEQARDEVLQVVGAIAG"
     gene            complement(9869..11716)
                     /gene="mutL"
                     /locus_tag="OO357_002959"
     CDS             complement(9869..11716)
                     /gene="mutL"
                     /locus_tag="OO357_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HCR5887829.1"
                     /translation="MPIQVLPPQLANQIAAGEVVERPASVVKELVENSLDAGATRIDI
                     DIERGGAKLIRIRDNGCGIKKDELALALARHATSKIASLDDLEAIISLGFRGEALASI
                     SSVSRLTLTSRTAEQQEAWQAYAEGRDMDVTVKPAAHPVGTTLEVLDLFYNTPARRKF
                     LRTEKTEFSHIDEIIRRIALARFDVTINLSHNGKIVRQYRAVPEGGQKERRLGAICGT
                     AFLEQALAIEWQHGDLTLRGWVADPNHTTPALAEIQYCYVNGRMMRDRLINHAIRQAC
                     EDKLGADQQPAFVLYLEIDPHQVDVNVHPAKHEVRFHQSRLVHDFIYQGVLSVLQQQL
                     ETPLPLDDEPQPAPRAIPENRVAAGRNHFAEPAAREPVAPRYSPAPASGSRPAAPWPN
                     AQPGYQKQQGEVYRQLLQTPAPMQKPKAPEPQEPALAANSQSFGRVLTIVHSDCALLE
                     RDGNISLLSLPVAERWLRQAQLTPGEAPVCAQPLLIPLRLKVSGEEKSALEKAQSALA
                     ELGIDFQSDAQHVTIRAVPLPLRQQNLQILIPELIGYLAKQSVFEPGNIAQWIARNLM
                     SEHAQWSMAQAITLLADVERLCPQLVKTPPGGLLQSVDLHPAIKALKDE"
     gene            complement(11726..13063)
                     /gene="amiB"
                     /locus_tag="OO357_002960"
     CDS             complement(11726..13063)
                     /gene="amiB"
                     /locus_tag="OO357_002960"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710034.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase AmiB"
                     /protein_id="HCR5887830.1"
                     /translation="MMYRIRNWLVATLLLLCTPVGAATLSDIQVSNGNQQARITLSFI
                     GDPDYAFSHQSKRTVALDIKQTGMIQGLPLLFSGNNLVKAIRSGTPKDAQTLRLVVDL
                     TENGKTEAVKRQNGSNYTVVFTINADVPPPPPPPPVVAKRVETPAVVAPRVSEPARNP
                     FKTESNRTTGVISSNTVTRPAARATANTGDKIIIAIDAGHGGQDPGAIGPGGTREKNV
                     TIAIARKLRTLLNDDPMFKGVLTRDGDYFISVMGRSDVAHKQNANFLVSIHADAAPNR
                     SATGASVWVLSNRRANSEMASWLEQHEKLSELLGGAGDVLANSQSDPYLSQAVLDLQF
                     GHSQRVGYDVATSMISQLQRIGEIHKRRPEHASLGVLRSPDIPSVLVETGFISNNSEE
                     RLLASDDYQQQLAEAIYKGLRNYFLAHPMQSAPQGATAQTASTVTTPDRTLPN"
     gene            complement(13082..13543)
                     /gene="tsaE"
                     /locus_tag="OO357_002961"
     CDS             complement(13082..13543)
                     /gene="tsaE"
                     /locus_tag="OO357_002961"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HCR5887831.1"
                     /translation="MMNRVIPLPDEQATLDLGERVAKACDGATVIYLYGDLGAGKTTF
                     SRGFLQALGHQGNVKSPTYTLVEPYTLDNLMVYHFDLYRLADPEELEFMGIRDYFAND
                     AICLVEWPQQGTGVLPDPDVEIHIDYQAQGREARVSAVSSAGELLLARLAG"
     gene            complement(13515..15062)
                     /gene="nnr"
                     /locus_tag="OO357_002962"
     CDS             complement(13515..15062)
                     /gene="nnr"
                     /locus_tag="OO357_002962"
                     /EC_number="4.2.1.136"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710032.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional ADP-dependent NAD(P)H-hydrate
                     dehydratase/NAD(P)H-hydrate epimerase"
                     /protein_id="HCR5887832.1"
                     /translation="MTDHTMKKNPVSIPHTVWYADDIRRGEREAADVLGLTLYELMLR
                     AGEAAFQVCRSAYPDARHWLVLCGHGNNGGDGYVVARLAKAVGIEVTLLAQESDKPLP
                     EEAALAREAWLNAGGEIHASNIVWPESVDLIVDALLGTGLQQAPRESISQLIDHANSH
                     PAPIVAVDIPSGLLAETGATPGAVINADHTITFIALKLGLLTGKARDVTGQLHFDSLG
                     LDSWLAGQETKIQRFSAEQLSHWLKPRRPTSHKGDHGRLVIIGGDHGTAGAIRMTGEA
                     ALRAGAGLVRVLTRSENIAPLLTARPELMVHELTMDSLTESLEWADVVVIGPGLGQQE
                     WGKKALQKVENFRKPMLWDADALNLLAINPDKRHNRVITPHPSEAARLLGCSVAEIES
                     DRLHCAKRLVQRYGGVAVLKGAGTVVAAHPDALGIIDAGNAGMASGGMGDVLSGIIGA
                     LLGQKLSPYDAACAGCVAHGAAADVLAARFGTRGMLATDLFSTLQRIVNPEVTDKNHD
                     ESSNSAP"
     gene            15061..16200
                     /gene="queG"
                     /locus_tag="OO357_002963"
     CDS             15061..16200
                     /gene="queG"
                     /locus_tag="OO357_002963"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710031.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HCR5887833.1"
                     /translation="MSEPLDLNQLAQKIKQWGLELGFQQVGITDTDLSESEPKLQAWL
                     DKQYHGEMDWMARHGMLRARPHELLPGTLRVISVRMNYLPANAAFASTLKNPKLGYVS
                     RYALGRDYHKLLRNRLKKLGEMIQQHCVSLNFRPFVDSAPILERPLAEKAGLGWTGKH
                     SLILNREAGSFFFLGELLVDIPLPVDQPVEEGCGKCVACMTICPTGAIVEPYTVDARR
                     CISYLTIELEGALPEELRPLMGNRIYGCDDCQLICPWNRYSQLTTEDDFSPRKPLHAP
                     ELIELFAWSEEKFLKVTEGSAIRRIGHLRWLRNIAVALGNAPWDETILTALESRKGEH
                     PLLDEHIAWAIAQQIERRNACIVEVQLPKKQRLVRVIEKGLPRDA"
     gene            complement(16183..16236)
                     /locus_tag="OO357_002964"
     CDS             complement(16183..16236)
                     /locus_tag="OO357_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887834.1"
                     /translation="MRFYFYSQAVDESGVTR"
BASE COUNT         3737 a         4702 c         4133 g         3894 t
ORIGIN      
        1 atctaaccct cttcttttaa agagggtatt gatcactctg ccactttttt cttcgccgca
       61 cgcttcgctt tggtcgctgc ggctattttc tgcgttttcg ccgatggctt tttcgctttt
      121 ctcgcgtctt tcttcgccgc tttcggcttc gtttttttct caccgcggaa ggcgctgtct
      181 ggctcaaagt ttaccttttt accgacctga caacgcttgc cgcctttttt gcctgcatcg
      241 ccttttttcg ctttctcgcg cgccgtttta ccgacgttgc gcggtgcgcg ttcactggag
      301 atcagactaa agtcgatttt gcgctcgtcc atattaaccg cttcgacgcg aacttccacg
      361 cgatcgccca ggcgataagt ctggccgctg gattccccca tcaggcgttg ccctacctgg
      421 tcaaagcgat agtagtcatt gtccagcgaa gagacatgga ccagaccatc aatgaacaag
      481 tcgtccagac ggacgaagaa gccaaagcca gtgacgctgg aaattacgcc tttaaagacg
      541 ttacctacct ggtcgagcat gaagtcacac ttcagccagt cagcgacatc gcgcgttgct
      601 tcgtcggcac gacgttccgc catcgaacag tgctgaccca gttgcaacat ctcttccatc
      661 gaataatggt agccgccggt ttcagtggtg ttgccctgat gcccctgctc tttcgccagc
      721 agatatttaa tggcgcggtg cagcgtcagg tcaggataac gacgaatcgg cgaagtaaag
      781 tgcgcatagg actgcaatgc cagaccgaag tgaccacggt tttctggatc gtaaatcgcc
      841 tgtttcatcg agcgtagcag catggtttgc agcatttctg catcaggacg gtcggcaacc
      901 gactccagca gttccgcgta gtcacgcggt tccggcttgt taccacccgg cagctccagc
      961 cccagctccg ccagcactga acggaaagag gtaatcgctt cggtgctcgg cttgtcgtga
     1021 atacggaaca gtgccggttc tttcgctttc tcaacgaaac gcgccgccga gatattcgcc
     1081 agaatcatgc actcttcaat taatttgtgc gcgtcgttac gctgggtctg ttcgatacgt
     1141 tcaatacggc gttcagcgtt gaaaatgaac ttcgcttctt cgctctcaaa tgagatccca
     1201 ccgcgttctt cacgggcttt atccagcact ttatagaggt tatgcaactc ttcgagatgc
     1261 ttaaccagcg gggcgtactg ctcgcgcaga tcctgatcgc cctgcagaat atgccagact
     1321 ttggtgtagg tcagacgcgc gtgagagctc atcaccgctt cgtagaattt gtagcccgtc
     1381 aggcggcctt tcgacgaaac cgtcatctcg cacaccatac acaggcggtc tacctgcggg
     1441 ttgagcgaac acaggccgtt agagagcact tccggcagca tcgggataac ctgcgaaggg
     1501 aagtacaccg acgtgccacg gttacgcgct tctctgtcca gcggcgttgg cggacgcaca
     1561 tagtagctga cgtcggcaat cgcgacccat aaacgccagc cgccgccgcg ttttttctcg
     1621 cagtaaactg catcgtcaaa gtcacgggcg tcttcgccat caatggtgac cagcggtaaa
     1681 tcgcgcaaat cgacacggcc cgcttttgct tcttccggca cttcttcttt cagccccgca
     1741 acctgttgct caacagcctg cggccagata tacggaattt catgggtacg cagagcgata
     1801 tcaaccgcca tgccggtgcc catattgtcg cccagcactt cgacgatttt acccaccgct
     1861 ttggtgcggc gagtcggacg ctgagtcagt tcgactacga ccacaaagcc catccgcgcg
     1921 cccatgatct gatcgggcgg gattaagata tcgaagctca gacggctatc gtcaggaacc
     1981 acaaagccga cgcccgcttc ggtaaagtag cgaccaacaa tctggctggt ttttggcacc
     2041 agtacgcgga caatacgcgc ttcacgacga cctttacggt cagcacccag cggctgcgcc
     2101 agcacctgat cgccatgaat gcaggttttc atctgctcgc tggagagata caaatcatct
     2161 ttacgccctt caacccgcag aaagccgtag ccatcacggt gaccaataac ggtacctttc
     2221 accaggtcga ggcgttccgg cagcgcatag cactgacgac gagtgaagac cagttgacca
     2281 tcgcgctcca tcgcgcgcag gcggcgacgc aggccttcaa gctgctcttc gccttcaatg
     2341 tgcagttcta ccgccagctc atcacggctg gccggttttt cacgtttggt taaatgttcg
     2401 aggataaatt cccgactagg gatgggattc gcgtattttt cagcttcgcg ttcctggaaa
     2461 ggatcttgtg acatctcggt tcctccgttg tcatctctga tgaagatttt cgtcactcca
     2521 ccagcaataa tttataaagc ggttgattct cttcaaccaa atcggcaagc gtgtagttat
     2581 ccagttccgt aagaaaactt tgcacggcct tagaaagcgc ctgtttcaac cgacaggcag
     2641 gtgtaatgtg gcaaaactca ctgctgcaat tcaccagcga taagggctcc agctcgcgca
     2701 ccacatcacc aatacgtatc gcactcgccg gtttacccag gcgaatgccg ccattttttc
     2761 cacgaacagc agtcacgtag ccggcacgac taagttgatt gattattttg accatatgat
     2821 tacgggagac gccgtagacg tcagtcactt cagaaatact ggtcatccgc ccttctggca
     2881 atgacgccat gtagatcagc gcacgtaatc cgtaatcagt gaaactcgtt aactgcacat
     2941 caacctcaaa agggaaatcg ggaaaaaata catttatatt gatgataaac cagccacaag
     3001 ctgtgtcgct aatttatttc agttcgggga aggaaaaaag cgaggattta acactgtgtc
     3061 ggatagcgta atggcttaac cgacacagca atgtatcaca ctgttagccc ggcaggcaaa
     3121 atatctgcca ggcgtaccag aattacgcgt cgaacgggtc gcgcagaatc atggtttcag
     3181 tacgatccgg accggtagag atgatatcga tcggcacacc ggtcagctct tcaatacgct
     3241 tgatgtagtt cagcgccgcc tgcggcaggc cgctacgatc tttcacgccg aaagtggatt
     3301 cagaccagcc cggcatggtt tcgtaaatcg gctctacacc tttccagtcg tcagctgcca
     3361 gcggagtggt agtcacttcg cgaccatccg gcatacggta agccacgcag agtttaacct
     3421 ctttcaggcc atccagaacg tccagtttag tcaggcagaa gccagacagg gagttcagct
     3481 gtaccgcacg acgaacggca acggtgtcca gccagccggt acgacgacga cgacccgtag
     3541 ttgcaccgaa ttcgttaccc tgcttgcaga ggaactcgcc agtttcatca aacagttcgg
     3601 ccgggaaagg acctgcacct acacgagttg agtaagcttt gaggataccc agaacgtaat
     3661 caacataacg cgggcccagg ccggaaccgg tcgccacgcc accagcagtg gtgttggaag
     3721 aagttacgta cggataagta ccgtggtcga tatccagcag cgtaccctgc gcaccttcga
     3781 acatgacgaa atcgccacgc tgacgcgcct ggtcgagcag gtcagaaacg tcaaccacca
     3841 tagaagtcag gatgtcggca acagccatcg tatcatccag aactttctgg taatcaaccg
     3901 cttcagcttt gtagtagtta accaactgga agttgtgata ttccatcact tctttcagtt
     3961 tttcagcgaa ggtttctttg tcgaaaaggt cgccaacacg cagaccgcga cgtgccactt
     4021 tatcttcata agcaggcccg ataccacgac cggtggtgcc gatcgctttc gcgccacgcg
     4081 ctttctcacg cgcgttatcc agagcaacgt gataatcaag gatcagcgga catgcttcag
     4141 acagcagcag acgctcacga acggggatgc cacggtcttc cagttctttc atctctttca
     4201 tcagcgcggc cggagacagc acaacaccgt taccgatgat gctggttaca ttctcgcgga
     4261 gaatacctga tggaataaga tggagaacgg ttttttcacc gttgattacg agagtatggc
     4321 ctgcgttgtg accgccctgg tagcgtacaa catatttagc ccgttcagtc agaagatcga
     4381 cgatcttacc tttaccttcg tcaccccatt gggtgcccag tacgacgacg ttgttaccca
     4441 tttttcaaaa tcaccgtttg cttaaaaatg gattctacca tcgctttttc agagttacag
     4501 cactttttgc actcaaaaat gacctcgatc agtctgtttt ttgttcagcc aatcgttttc
     4561 ctcaacatgt agtagaccac aacgcccgca accacaagtc caccgccaaa acgacgtaaa
     4621 atattatcgg gcaaattggt catcgcagag atcatcttct tccatgcctt cgggtaaagc
     4681 atcggcccta aaccttccag taccaaaacc agggcaagcg ccagccagat tgtcgaattc
     4741 attcattatc cttatagaaa aagaaaacca ccgacatccc tgaggatgcg gtggctttat
     4801 tgacctgtac cgcagtcgtt atattaacgc gttgcggaag tcggcgtctt catgtagcgg
     4861 aagaaatcgc tatccgggct catgaccatc acgtcctgat tgccagagaa gctgttctca
     4921 taagcacgca ggctacggat gaatgcgtag aagtccggat ctttactgaa tgcatcagca
     4981 aacagtttgg ctgcttcggc atcgccttca ccacgcataa tgcggccctg acgctcagct
     5041 tctgccagcg ttctggtcac ttcatagtcg gcagtcgcgc gcagtttttc cgcttcttcc
     5101 tgaccttgtg aacggtgacg acgcgctacc gcttcacgct cggcgcgcat acggttgtag
     5161 atcgcttcag acacttcggt cggcaggttg atctgcttga tacgcacatc gacaacttca
     5221 atacccagcg ccgccatact gttcgggttg atgaccggaa ctttgccctt cgtctcagcc
     5281 gttacgcgct ctgccgcttc ggcaatggcg ttatctgctg ccggggtagt aacttcatct
     5341 tctgtacccg cagaaccgga gttcagcgcg tcacgtactt cgagggtcag acgaccacgg
     5401 gaatcggtga cgatatcttt cacgtccagg cgaccaattt cagaacgcag acggtcagag
     5461 aacttacgtt tcaacagcac ttccgcttgc gaaatgtcgc caccacccgt tgccaggtag
     5521 taacggctga aatcgctgat gcgccatttg atgtaagagt cgacgatcag gtctttcttc
     5581 tctttggtca caaagcggtc ggcctggttg tccatggtct gaatacgtgc gtcgagcatt
     5641 ttcaccgttt caatgaacgg tatcttgaaa tgcagaccag gctcataaac caaaggtttg
     5701 ttgtcatcgt cacgcagtac cttaccaaaa cgcagcgtaa taccgcgctc accttctttg
     5761 acgacaaaga cagacatgta aagcactacc agcacgatga tgataatcgc gataactgac
     5821 ttacgcatcg ttattccccc tgacgctggt agtcgttacg ctgcgcgttg gcgcggcgtt
     5881 ggtccataat atcgccctga ctggtggacg acgtgttgct tgctccactg gttgtggagg
     5941 aagaggctgg cggcagacgc agcagattgc tggcaccgtt atcgctcttc gccgcagggg
     6001 cgttaccacc tttcagcatc tggtctaacg gcagaaccat caggttgcca cctttatcgt
     6061 taaccagcac tttgcgggtg ttacccaaca ctttttccat cgtctcgata tacagacgct
     6121 cgcgagtaat ttccggcgcg gctttatatt ccggcagaag tttagcaaag cgcgccactt
     6181 caccctgagc ttccaggatg gtctgggcct tgtacgcacg cgcctcttcg aggatacgtt
     6241 gcgcctgacc gttcgcacgc ggctgaactt cgttggaata cgcttctgct tcacgaatgt
     6301 attgctgttc gttttcacgc gcggcaatcg catcgtcaaa cgccgctttt acttcttccg
     6361 gcggacgagc agcctggaag ttgacgtcca gcagcgtgat acccatgtca tacggacgaa
     6421 tcgtctcttc cagttcgcgc tgagtatcgc tacgaatcac ggtacgacct tccgtcagaa
     6481 tgcggtccat ggtgtattta ccgataactc cacgcagggc gctgtcggta gcctgacgca
     6541 ggctgtcatc cgggctggtc acgctataca gatatttttc cggattggtg acgcggtact
     6601 gcacgttcat ctcaacgcgc actacgttct cgtccgacgt cagcatcaca ccagaagcgg
     6661 ccagttcacg cacggcttcc acgttcaccg gtttgacttc gtcgataaac gtcggtttcc
     6721 agttcagacc cggctcaacc agatggctga atttaccaaa gcgtgttacc acgccgcgtt
     6781 cggcttcttt aatggtatag aaaccactgg ccgcccagat aatgacaatc gctgccgctg
     6841 cgatggtaac gacacgaccg ccaagctgcg ggcgcgggcc ttgcgatgaa ctgccaccgc
     6901 cagatccggt gcctttaccg ccgcccagac caccgagctt tttgctcagt ttgcggaaga
     6961 tatcatctaa atcaggtggc ccttgatcgc gaccgccttt gtttccattt ccctcagagt
     7021 tgccgccagg tttgctgctt ccccacgggt cgcggtcttg tccgttatta ccgggctgat
     7081 tccacgccat gtttgtgctc catatttgtt atgcggtgat ccctgttgtc tttcgacgcc
     7141 agaggatatg actccacgct tcagacgcta cgccgttaga tcaggtaatc gatcaacgcc
     7201 ggttcttgtt tacagaggcg acgccagtca acgatcggca tacgaacttg cagacttacg
     7261 ctgccgtcct cctccatcca ctctttttct attgcctgaa gctgataaaa acgactcctc
     7321 agacgccctt cctgcggtgg cagacgcaat gtatgctgcg ccacctcgcc ggaaagccgc
     7381 tccgtcaaag cctgaaaaag ctgtggtatc cccgctccgg tctgtgcgga aagccagaca
     7441 cggatcggtt tgttctcttc gtcccgatca atacgcggtt cgaaatcttc cagcatatcg
     7501 atcttgttca tcaccagcag ggttgggatc tcgtgagcgt cgatctcttc aagaaccgta
     7561 ttcaccgctt cgatgttttc ttgtacacgc acatccgccg catcaatgac gtgcagcagt
     7621 aatgtggctt gccgcgtctc ttgtaacgtg gctttaaatg ccgccaccag atcgtgcggc
     7681 aggtggcgaa taaaccctac ggtatctgca agtacggttt caccgacatc tgcaacgtca
     7741 atacgccgca acgtcgggtc gagggtggca aacaactggt ctgccgcgta gacccgcgct
     7801 tcggtgatgc gattgaaaag ggtagattta ccggcgttgg tatatcccac cagcgaaaca
     7861 gtaggaacgt cggctttgat acgcgattgc cgcccctgct cacgctgctt ttcaactctt
     7921 tccaggcgcg actgtatctg cacgatgcga ttacgcaaca aacgacggtc ggtttcgagc
     7981 tgggtttcac ccggaccacg caaacctatc ccgcctttct gtctttcaag gtgggtccag
     8041 ccacgcacca ggcgcgtagc cagatggcgc agctgcgcca gctcaacctg caacttaccc
     8101 tcatgggtac gcgcacgttg ggcgaaaata tctaaaataa ggccggtgcg gtcgataaca
     8161 cgacactcgc acaaacgctc caggttacgc tcttgcgccg ggctcagggc atggtcaaaa
     8221 agaacgaccg aagcacccat cgctttgaca gcttccgcaa tttcaactgc tttaccttca
     8281 cctacaaaat actttgggtg cggcgcttta cggctaccgg taatcacctg caatgcttcg
     8341 acaccggcgg aagagaccag agattcaaac tcctggaggt cttccatatc tttgtcttgc
     8401 gtaaaataga tgtgtaccag taccgcctgc tcaccagcat cataacggtc aaacaagcgt
     8461 ataaccctct aaatagatca gcggggaacg caggatcgct ggctccccgt gtaaaaaaac
     8521 agcccgaaac cttattcggt ttcttcgctg tcctgttgcg cggaagtatt ctgcgcgctg
     8581 ctaccatgat ggtagttact gctggtaccg ccaccggcgt tgttactgtg atgagaaacc
     8641 gggcgagatg ggacaacagt agaaatcgcg tgcttgtaaa ccatctggct gaccgtgttt
     8701 ttcaacagga tcacgaactg atcaaaagac tcgatttgcc cttgcagctt aataccattc
     8761 accaaataaa tagaaactgg aacacgttcc cgacgcagtg cgttcaggaa cggatcttgt
     8821 aaagattgcc ccttagccat tctctctttt ccttatatgc ttatttgtac tttgaacctt
     8881 tcgattctga aaaaattgcg cacgatacgt ctcaattgta cacattcagc ctgcgatagc
     8941 accaacaacc tgtaatactt cgtcacgcgc ctgttctggt ttttcactgt caagccagtg
     9001 aaccccttcc caaccacgca gccaggttat ctgccgcttc gccaactgtc tcgtggcgca
     9061 aacacctcga taaaccattt catcgtatga gatttcgcct tcaaggtaag accacatctg
     9121 gcgataaccc acgcaacgaa tggaaggcaa gtccgtatgc aaatctcctc gggcaaaaag
     9181 cgcccggact tctgcttcaa aacctgaagc caacatctga tgaaaacgct gctcaatgcg
     9241 ttgatggagc agttcacggc tcgccggggc gatggcgaac tgatgcacct gatacggtag
     9301 agcgtctcct gacgtttgcg tcagttccgt taaagtttta cccgaaatga aaaaaacttc
     9361 cagtgcccgg gaaagccttt gtggatcatt tggatgaatc cttgctgccg caaccggatc
     9421 tacctcctga agttgacgat gcaatgactc ccaaccttgc tctgccgcct gttgctcaat
     9481 tctggcccgc acttccgggt ctgccgacgg tagcggcgac aacccttcca gcaatgcctt
     9541 gaaatacaac atcgtaccgc ccactaacag tgggatccgc cccgccgcgg tgatatcggc
     9601 catttccgcc agcgcatcgc ggcgaaaatc agcagccgag taagcctgcg acggatcgcg
     9661 aatatccagc aatcggtgcg gcgcggcgag taactcttca gcgttcggct tcgccgtccc
     9721 gatatccatc cctttgtaaa taaggacaga atcaacgctt atcaactcta ctggtaaaat
     9781 tttacgcagc tcaatggcta acgccgtttt accggaggcc gtcggcccca tcaaaaaaat
     9841 cgccttaggc aggctcgcct tactgatatc actcatcttt cagggctttt atcgccggat
     9901 gtaaatcaac agattgtaac agaccacccg gcggcgtttt cacaagttgc ggacataacc
     9961 gttccacgtc cgccagcagg gttatggcct gtgccattga ccactgcgca tgttcgctca
    10021 tcagatttcg tgcaatccac tgcgcaatat tgccaggttc gaatacggac tgcttcgcca
    10081 ggtagcctat cagttcagga atcaagattt gtaaattttg ttggcgtaag ggtaaaggca
    10141 cggccctgat ggtcacatgc tgtgcatctg actggaaatc aatacccaat tccgctaggg
    10201 cagactgcgc tttttctaat gccgattttt cttcgccaga aacttttagc cgcaacggaa
    10261 tcagcagcgg ctgggcgcaa acgggcgctt cacccggcgt caattgtgcc tgacgcagcc
    10321 aacgttctgc cactggcaag gataaaagtg aaatgttgcc atcgcgctcc agcaacgcac
    10381 agtcggaatg gacgatagtc agtacccgac caaaactctg actgttcgcc gcaagtgcag
    10441 gttcctgcgg ttccggcgct tttggttttt gcatcggcgc gggcgtttgc aaaagctggc
    10501 gatacacttc accttgctgt ttctggtagc ctggctgcgc attcggccag ggggcagccg
    10561 gacgactgcc tgaggcgggc gcaggagagt agcgcggagc taccggctca cgagctgccg
    10621 gttctgcaaa gtgattgcgc cccgccgcca cgcggttttc cgggatggca cgcggtgcgg
    10681 gttgtggttc atcgtccagc ggtagcggcg tttccagttg ctgttgtagc acgctcagca
    10741 cgccctgata gataaaatca tgcaccagac gcgactgatg gaaacgcact tcgtgtttgg
    10801 cggggtgtac gttgacgtcc acctgatgcg ggtcgatctc cagatacaac acaaatgccg
    10861 gttgctgatc ggcccccagt ttgtcttcgc aggcctggcg gatcgcatga ttgatcaggc
    10921 gatcgcgcat catgcgacca ttcacgtagc aatactgaat ttccgccagt gcgggcgtag
    10981 tgtgatttgg atcggccacc cagccgcgca gggtgagatc gccgtgttgc cattcaatcg
    11041 ccagcgcttg ttcgagaaaa gcggtgccgc aaatcgcgcc taagcgccgt tctttttgcc
    11101 cgccttccgg cactgcgcgg tactgacgca caattttacc gttatgcgac aggttgatcg
    11161 tgacgtcgaa acgcgccagc gcaatgcggc ggatgatttc atcaatatgg ctaaattcgg
    11221 ttttctcggt gcgcaggaat ttgcgccgcg ccggggtgtt gtagaacaga tccagcacct
    11281 ccagcgtcgt tcccaccgga tgcgctgccg gtttaaccgt cacgtccatg tcgcgtcctt
    11341 cggcataggc ctgccaggct tcctgctgtt ctgcggtgcg tgaagtgagc gtcaggcggg
    11401 aaaccgaact gatactcgcc agcgcctcac cgcgaaagcc caggctgata atggcttcga
    11461 gatcgtccag agaggcgatt ttactggtgg catgacgagc cagcgccagc gccagctcat
    11521 cttttttgat accgcagccg ttatcacgaa tgcggataag tttcgcccca ccgcgttcga
    11581 tatcaatatc gatacgcgtc gcacctgcat cgaggctgtt ttccactagt tctttgacta
    11641 ccgacgcagg tcgctcgacc acctcacctg cggcaatctg gttcgccagt tgtggcggta
    11701 agacctgaat tggcatcaat cgtccttagt ttggcagcgt gcgatctggc gtcgtcaccg
    11761 tactggcagt ttgtgccgtt gcaccctgcg gcgcagattg catcggatgc gcaaggaagt
    11821 aattgcgcag gcctttgtaa atggcttctg ccagctgttg ttggtaatcg tcgctcgcca
    11881 gcaaacgttc ttcgctgttg ttgctgataa aaccggtttc gaccagtact gatgggatat
    11941 ccggcgagcg cagaacgcca aggctggcgt gttctggtcg acgtttatgt atttcgccaa
    12001 tgcgttgcaa ctgactgatc atactggtcg ctacatcata ccctacccgc tgggaatgac
    12061 cgaactgtaa atccagcacc gcctggctta aataggggtc agactgactg ttcgccagca
    12121 catcacccgc cccacccagc agctccgaca gtttctcgtg ctgctccagc cagctggcca
    12181 tttcactgtt ggcgcgacgg ttagagagca cccatacgga agcgccagtc gcactacggt
    12241 tcggtgcggc atcagcgtga atcgacacga ggaaattggc gttttgctta tgtgccacat
    12301 cactgcgccc catcaccgag ataaagtaat ccccgtcacg ggttaaaacg cctttaaaca
    12361 tcggatcgtc attgagcaaa gtacgcaatt tacgcgcgat ggcgatggtg acatttttct
    12421 cccgcgtacc accggggccg atagcgccag ggtcctgacc gccgtgtccg gcatcaatag
    12481 cgatgataat tttatcgcca gtgttagccg tcgcgcgcgc tgccggacgc gttaccgtat
    12541 tactgctgat aacacccgta gtgcggttac tttccgtttt aaacggattg cgcgccggtt
    12601 cgctgacgcg cggtgcgaca accgcaggcg tttcaacgcg tttcgcaacc acgggcggcg
    12661 gaggaggcgg tggcggcaca tcggcgttaa tcgtaaagac gacagtgtaa ttgctgccat
    12721 tctgccgctt caccgcttcg gttttaccgt tttcggtaag atcgaccacc agccgtagcg
    12781 tttgtgcatc tttaggcgtt ccagagcgaa tcgccttcac cagattattg ccgctgaaca
    12841 acaacggcag tccctgaatc atgcccgttt gtttgatatc gagcgccacg gtgcgtttgc
    12901 tttgatggct aaacgcataa tcaggatcgc caataaaact caacgttatc cgcgcctgtt
    12961 gattaccgtt agaaacctga atatcagaga gcgtcgcggc acccaccggc gtgcacagca
    13021 gcagcagcgt cgctaccaac caatttctga tgcgatacat catcccgcca cctttcaaag
    13081 gttaaccggc taaacgcgcc agcaacaatt cacccgcaga ggaaaccgca ctcacgcgcg
    13141 cctcacggcc ttgtgcctga taatcaatgt gtatttcgac atccgggtca ggaagaacac
    13201 ctgtaccttg ttgtggccac tccaccaggc agatggcatc gttggcaaaa taatcgcgga
    13261 tccccataaa ctccagctcc tcgggatcgg caaggcggta caaatcaaag tgatagacca
    13321 ttaagttgtc gagcgtatag ggttcgacca gcgtataagt ggggcttttg acattaccct
    13381 gatgacccag agcctgtaaa aagccccggc taaaggtggt tttacctgcg cctaaatcgc
    13441 catacagata gattacggtt gcgccatcgc aggctttcgc tacccgctcg cccaggtcta
    13501 atgttgcctg ctcatcaggg agcggaatta ctcgattcat catggttttt atcagtcact
    13561 tccgggttaa caatacgctg tagcgtggaa aagagatcgg ttgccagcat cccgcgcgtt
    13621 ccaaaacgcg ccgccagtac gtcagctgcc gcaccgtgcg cgacacagcc tgcacaggct
    13681 gcatcatacg gcgacagttt ttgcccaagc aatgcgccaa taataccaga gagcacatcg
    13741 cccatgccgc cgctcgccat gcctgcattt ccggcatcaa taatgcctaa agcgtcagga
    13801 tgggcggcga ccacggttcc ggcacctttc agcaccgcta cgccgccata acgttgtacc
    13861 agacgtttgg cgcaatgtaa gcggtcactt tcaatttcag cgacggaaca gcctaacaac
    13921 cgtgcggcct cgctaggatg cggcgtgatc acgcgattgt gacgcttatc gggattgatt
    13981 gccagcaggt tcaatgcatc ggcatcccac aacatcggtt tgcgaaaatt ctcaactttt
    14041 tgcagggctt ttttccccca ctcttgctgg cccagaccgg gaccaatcac caccacatcg
    14101 gcccattcca ggctttcggt aagagagtcc atagtcagtt catgcaccat caattctggt
    14161 cgtgcagtca gcagcggcgc aatgttctca ctgcgggtca gtactcggac taaaccagca
    14221 ccagcacgta gcgccgcttc ccccgtcata cgaatagccc ccgccgtgcc gtgatcgcca
    14281 ccgataatca ccagccgccc gtgatcgcct ttatgcgaag tcgggcgacg cggtttcagc
    14341 cagtgagaaa gttgttctgc cgaaaaccgc tgaattttcg tctcctgacc tgccagccag
    14401 ctatccagcc ccagtgagtc aaaatgcagt tgtcctgtaa catcccgcgc ttttccagtg
    14461 agcaagccta gtttcagcgc aataaaagtg atggtgtgat cggcgttgat cactgcgcct
    14521 ggcgtagcgc cggtttcagc cagcaggccg gaagggatat caaccgccac aatcggcgca
    14581 ggatgggaat tagcgtggtc gattaactgg ctaatggatt cgcggggcgc ttgctgcaag
    14641 ccggtaccga gcagcgcatc aacaatcaga tctaccgatt cgggccagac aatattcgaa
    14701 gcatggatct cgccacccgc gtttaaccat gcttcgcgtg ccagcgcggc ctcttccggc
    14761 aacggtttgt cgctctcctg ggccaacaac gtgacctcaa tgccgaccgc tttggccagt
    14821 cgcgcgacca cgtagccatc gccgccgtta ttaccatgac cgcacagcac cagccagtgg
    14881 cgggcgtcag gatacgccga acgacacacc tggaatgcgg cctcgccagc gcgaagcatc
    14941 agctcataga gtgtgagccc cagcacatct gccgcctcgc gttctccgcg gcggatatcg
    15001 tcggcgtacc agacggtgtg tggtatactt acggggtttt tcttcattgt atggtccgtc
    15061 atgtcagagc ccctcgatct caatcagtta gcgcaaaaaa ttaaacagtg ggggctggaa
    15121 ctgggctttc agcaggtagg tattaccgat accgatctca gcgagtccga gcccaaactg
    15181 caagcatggc tggacaaaca ataccacggc gaaatggact ggatggcacg tcacggtatg
    15241 ctgcgcgctc gcccccatga gttattaccc ggtacgctgc gtgtaatcag cgtgcggatg
    15301 aattaccttc ctgctaacgc cgcatttgcc agcacgctga aaaaccccaa actcggctat
    15361 gttagccgtt atgcgctggg ccgtgactat cacaaacttc tgcgcaaccg actcaaaaag
    15421 ctgggcgaga tgattcagca acattgtgtt tcgctgaatt ttagaccgtt tgtcgattct
    15481 gcgcctattc tcgagcgccc gttagctgaa aaagctgggc tcggctggac aggtaagcac
    15541 tcacttatcc tcaatcgcga ggccggttcg ttcttctttt taggcgaatt gctggtcgat
    15601 attccgctgc ccgtggatca accagtcgag gaaggatgcg gcaaatgcgt ggcctgtatg
    15661 acgatttgcc cgaccggtgc catcgtcgag ccatataccg tcgatgctcg ccgctgtatc
    15721 tcttatctca ccatcgaact tgaaggggcg ctcccggaag agttgcgacc gttaatggga
    15781 aaccgtattt acggttgcga tgactgccag cttatctgcc cgtggaatcg ctattcgcaa
    15841 ctcactacag aagacgattt cagcccgcgt aagccgctac acgcaccgga actcattgag
    15901 ttatttgcct ggagcgaaga gaagttttta aaagtcacgg aaggatcggc gattcgccgt
    15961 ataggtcacc tgcgctggct gcgtaatatc gccgtggcat taggcaatgc cccttgggat
    16021 gaaacgattt tgacagcgct ggaaagtcgt aaaggtgagc acccacttct cgatgagcac
    16081 atagcgtggg cgattgcgca gcaaatagag agacgaaatg cgtgcatagt cgaagtgcaa
    16141 ttgccgaaaa aacagcgtct ggttcgggtg attgaaaaag ggttaccgcg tgacgcctga
    16201 ttcatccaca gcttgtgaat aaaaataaaa acgcattgca attcaagacg aataaaatcg
    16261 tcaagtgatc gactcgacaa aacggaatct tgatttttaa cttatttatc aataggttaa
    16321 aaaaatacca ttcaccgtgc gaagtttctt tgaaattaag attacagcct gaggctgtgg
    16381 ataactctgt ttacaagagt ttgtcagaga caacaaaaac cagcaataac gcatcgcgtc
    16441 gctgtagata ttttattgag agaaga
//
LOCUS       DAJTRC010000083        16391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.083, whole genome shotgun sequence.
ACCESSION   DAJTRC010000083 DAJTRC010000000
VERSION     DAJTRC010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 16391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.083"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(89..2065)
                     /gene="speA"
                     /locus_tag="OO357_002965"
     CDS             complement(89..2065)
                     /gene="speA"
                     /locus_tag="OO357_002965"
                     /EC_number="4.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic arginine decarboxylase"
                     /protein_id="HCR5887835.1"
                     /translation="MSDDMSMGLPSSAGEHGVLRSMQEVAMSSQEASKMLRTYNIAWW
                     GNNYYDVNELGHISVCPDPDVPEARVDLAQLVKTREAQGQRLPALFCFPQILQHRLRS
                     INAAFKRARESYGYNGDYFLVYPIKVNQHRRVIESLIHSGEPLGLEAGSKAELMAVLA
                     HAGMTRSVIVCNGYKDREYIRLALIGEKMGHKVYLVIEKMSEIAIVLDEAERLNVVPR
                     LGVRARLASQGSGKWQSSGGEKSKFGLAATQVLQLVETLREAGRLDSLQLLHFHLGSQ
                     MANIRDIATGVRESARFYVELHKLGVNIQCFDVGGGLGVDYEGTRSQSDCSVNYGLNE
                     YANNIIWAIGDACEENGLPHPTVITESGRAVTAHHTVLVSNIIGVERNEYTVPTAPVE
                     DAPRALQSMWETWQEMHEPGTRRSLREWLHDSQMDLHDIHIGYSSGTFSLQERAWAEQ
                     LYLSMCHEVQKQLDPQNRAHRPIIDELQERMADKMYVNFSLFQSMPDAWGIDQLFPVL
                     PLEGLDQVPERRAVLLDITCDSDGAIDHYIDGDGIATTMPMPEYDPENPPMLGFFMVG
                     AYQEILGNMHNLFGDTEAVDVFVFPDGSVEVELSDEGDTVADMLQYVQLDPKTLLTQF
                     RDQVKKTDLDAELQQQFLEEFEAGLYGYTYLEDE"
     gene            complement(2074..2205)
                     /gene="yqgB"
                     /locus_tag="OO357_002966"
     CDS             complement(2074..2205)
                     /gene="yqgB"
                     /locus_tag="OO357_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417414.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response protein YqgB"
                     /protein_id="HCR5887836.1"
                     /translation="MKKKPVAQSERQHTLLENPCAYGLLSQFQAATVVNCFTLNKII"
     gene            2341..2556
                     /locus_tag="OO357_002967"
     CDS             2341..2556
                     /locus_tag="OO357_002967"
                     /inference="COORDINATES: protein motif:HMM:NF037550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887837.1"
                     /translation="MGITSAGMQSRDAECGERVFTRTVRQVKQQTTVHYFVSPPRPPV
                     KTNPQAKTLISTRLEVATRKKRRVLFI"
     gene            2860..4014
                     /gene="metK"
                     /locus_tag="OO357_002968"
     CDS             2860..4014
                     /gene="metK"
                     /locus_tag="OO357_002968"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HCR5887838.1"
                     /translation="MAKHLFTSESVSEGHPDKIADQISDAVLDAILEQDPKARVACET
                     YVKTGMVLVGGEITTSAWVDIEEITRNTVREIGYVHSDMGFDANSCAVLSAIGKQSPD
                     INQGVDRADPLEQGAGDQGLMFGYATNETDVLMPAPITYAHRLVQRQAEVRKNGTLPW
                     LRPDAKSQVTFQYDDGKIVGIDAVVLSTQHSEEIDQKSLQEAVMEEIIKPILPAEWLT
                     SATKFFINPTGRFVIGGPMGDCGLTGRKIIVDTYGGMARHGGGAFSGKDPSKVDRSAA
                     YAARYVAKNIVAAGLADRCEIQVSYAIGVAEPTSIMVETFGTEKVPSEQLTLLVREFF
                     DLRPYGLIQMLDLLHPIYKETAAYGHFGREHFPWEKTDKAQLLRDAAGLK"
     gene            4451..5845
                     /gene="galP"
                     /locus_tag="OO357_002969"
     CDS             4451..5845
                     /gene="galP"
                     /locus_tag="OO357_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose/proton symporter"
                     /protein_id="HCR5887839.1"
                     /translation="MPDAKKQGRSNKAMTFFVCFLAALAGLLFGLDIGVIAGALPFIA
                     DEFQITSHTQEWVVSSMMFGAAVGAVGIGWLSFKLGRKKSLMIGAILFVAGSLFSAAA
                     PNVEVLILSRVLLGLAVGVASYTAPLYLSEIAPEKIRGSMISMYQLMITIGILGAYLS
                     DTAFSYTGAWRWMLGVIIIPAILLLIGVFFLPDSPRWFAAKRRFVDAERVLLRLRDTS
                     AEAKRELDEIRESLQVKQSGWALFKENSNFRRAVFLGVLLQVMQQFTGMNVIMYYAPK
                     IFELAGYTNTTEQMWGTVIVGLTNVLATFIAIGLVDRWGRKPTLTLGFLVMAAGMGVL
                     GTMMHIGIHSPSAQYFAIAMLLMFIVGFAMSAGPLIWVLCSEIQPLKGRDFGITCSTA
                     TNWIANMIVGATFLTMLNTLGNANTFWVYAALNVLFILLTLWLVPETKHVSLEHIERN
                     LMKGRKLREIGAHD"
     gene            5922..6419
                     /locus_tag="OO357_002970"
     CDS             5922..6419
                     /locus_tag="OO357_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HCR5887840.1"
                     /translation="MKTSRLPIAIQQAVMRRLREKLAQANLKLGRNYPEPKLSYTQRG
                     TSAGTAWLESYEIRLNPVLLLENSEAFIEEVVPHELAHLLVWKHFGRVAPHGKEWKWM
                     MESVLGVPARRTHQFELQSVRRNTFPYRCKCQEHQLTVRRHNRVVRGEAIYRCVHCGE
                     QLVAK"
     gene            6514..7221
                     /gene="endA"
                     /locus_tag="OO357_002971"
     CDS             6514..7221
                     /gene="endA"
                     /locus_tag="OO357_002971"
                     /EC_number="3.1.21.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708710.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease I"
                     /protein_id="HCR5887841.1"
                     /translation="MYRYLSIAAVVLSAAFSGPTLAEGINSFSQAKAAAVKVHADAPG
                     TFYCGCKINWQGKKGVVDLQSCGYQVRKNENRASRVEWEHVVPAWQFGHQRQCWQDGG
                     RKNCAKDPVYRKMESDMHNLQPSVGEVNGDRGNFMYSQWNGGEGQYGQCAMKVDFKEK
                     AAEPPARARGAIARTYFYMRDQYNLTLSRQQTQLFNAWNKMYPVTDWECERDERIAKV
                     QGNHNPYVQRACQARKS"
     gene            7301..8032
                     /gene="rsmE"
                     /locus_tag="OO357_002972"
     CDS             7301..8032
                     /gene="rsmE"
                     /locus_tag="OO357_002972"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003853382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HCR5887842.1"
                     /translation="MRIPRIYHPEPLTSHSHIALCEDAANHIGRVLRMGPGQALQLFD
                     GSNQVFDAEITSASKKSVEVKVLEGQIDDRESPLHIHLGQVMSRGEKMEFTIQKSIEL
                     GVSLITPLFSERCGVKLDSERLNKKLQQWQKIAIAACEQCGRNRVPEIRPAMDLEAWC
                     AEQDEGLKLNLHPRASNSINTLPLPVERVRLLIGPEGGLSADEIAMTARYQFTDILLG
                     PRVLRTETTALTAITALQVRFGDLG"
     gene            8045..8995
                     /gene="gshB"
                     /locus_tag="OO357_002973"
     CDS             8045..8995
                     /gene="gshB"
                     /locus_tag="OO357_002973"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HCR5887843.1"
                     /translation="MIKLGIVMDPIANINIKKDSSFAMLLEAQRRGYELHYMEMGDLY
                     LINGEARAHTRTLNVKQNYEEWFSFVGEQDLPLADLDVILMRKDPPFDTEFIYATYIL
                     ERAEEKGTLIVNKPQSLRDCNEKLFTAWFSDLTPETLVTRNKAQLKAFWEKHSDIILK
                     PLDGMGGASIFRVKEGDPNLGVIAETLTEHGTCYCMAQNYLPAIKDGDKRVLVVDGEP
                     VPYCLARIPQGGETRGNLAAGGRGEPRPLTESDWKIARQIGPTLKEKGLIFVGLDIIG
                     DRLTEINVTSPTCIREIEAEFPVSITGMLMDAIEARLQQQ"
     gene            9104..9667
                     /locus_tag="OO357_002974"
     CDS             9104..9667
                     /locus_tag="OO357_002974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005228750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HCR5887844.1"
                     /translation="MNLQHHFLIAMPALQDPIFRRSVVYICEHNTNGAMGIIVNKPLE
                     NLKIEGILEKLKITPEPRDESIRLDKPVMLGGPLAEDRGFILHTPPSNFASSIRISDN
                     TVMTTSRDVLETLGTDKQPSDVLVALGYASWEKGQLEQEILDNAWLTAPADLNILFKT
                     PIADRWREAAKLIGVDILTMPGVAGHA"
     gene            9667..10083
                     /gene="ruvX"
                     /locus_tag="OO357_002975"
     CDS             9667..10083
                     /gene="ruvX"
                     /locus_tag="OO357_002975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HCR5887845.1"
                     /translation="MSGTLLAFDFGTKSIGVAVGQRITGTARPLPAIKAQDGTPDWNL
                     IERLLKEWQPDEIIVGLPLNMDGTEQPLTARARKFANRIHGRFGVEVKLHDERLSTVE
                     ARSGLFEQGGYRALNKGKVDSASAVIILESYFEQGY"
     gene            complement(10259..11239)
                     /locus_tag="OO357_002976"
     CDS             complement(10259..11239)
                     /locus_tag="OO357_002976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HCR5887846.1"
                     /translation="MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDV
                     EELLREWLDDDQRAILLENGQLDFAVSLAENQRLRGSAFAQRQGISLALRLLPSHCPQ
                     LEQLGAPPVLPELLKSENGLILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
                     YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETG
                     HLVLATLHTRGAAQAVERLVDSFPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVAL
                     FELLINTPAVGNLIREGKTHQLPHVIQTGQKVGMLTFQQSYQQRVGEGRL"
     gene            11257..11961
                     /gene="yggS"
                     /locus_tag="OO357_002977"
     CDS             11257..11961
                     /gene="yggS"
                     /locus_tag="OO357_002977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate homeostasis protein"
                     /protein_id="HCR5887847.1"
                     /translation="MNDIAHNLAQVRDKISAAATRCGRSPEEITLLAVSKTKPASAIA
                     EAIDAGQRQFGENYVQEGVDKIRHFQELGVTGLEWHFIGPLQSNKSRLVAEHFDWCHT
                     IDRLRIATRLNEQRPAELPPLNVLIQINISDENSKSGIQLAELDELAAAVAELPRLRL
                     RGLMAIPALESEYVRQFEVARQMAVAFAGLKTRYPHIDTLSLGMSDDMEAAIAAGSTM
                     VRIGTAIFGARDYSKK"
     gene            11979..12545
                     /gene="yggT"
                     /locus_tag="OO357_002978"
     CDS             11979..12545
                     /gene="yggT"
                     /locus_tag="OO357_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmotic shock tolerance protein YggT"
                     /protein_id="HCR5887848.1"
                     /translation="MNTLTFLLSTVIELYTMVLLLRIWMQCAHCDFYTPFSQFVVKVA
                     QPIIGPLRRVIPAMGPIDSASLLVAYILSFIKAIVLFEVVTFLPIIWIAGLLILLKTI
                     GLLIFWVLLVMAIMSWVSQGRSPIEYVLIQLADPLLRPIRRLLPAMGGIDFSPMILVL
                     LLYAINMGVAEVLQATGNMLLPGLWMAL"
     gene            12542..12832
                     /locus_tag="OO357_002979"
     CDS             12542..12832
                     /locus_tag="OO357_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggU family protein"
                     /protein_id="HCR5887849.1"
                     /translation="MSAVTVNDDGLVLRLYIQPKASRDSIVGLHGDEVKVAITAPPVD
                     GQANSHLVKFLGKQFRVAKSQVVIEKGELGRHKQIKIINPQQIPPEIAALIN"
     gene            12840..13433
                     /locus_tag="OO357_002980"
     CDS             12840..13433
                     /locus_tag="OO357_002980"
                     /EC_number="3.6.1.66"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XTP/dITP diphosphatase"
                     /protein_id="HCR5887850.1"
                     /translation="MQKVVLATGNAGKVRELASLLSDFGLDIVAQTDLGVDSAEETGL
                     TFIENAILKARHAAKVTGLPAIADDSGLAVDALGGAPGIYSARYSGEDATDQKNLQKL
                     LETLKDVPDDQRQARFHCVLVYLRHAEDPTPLVCHGSWPGVITREPAGTGGFGYDPIF
                     FVPSEGKTAAELTREEKSAISHRGQALKLLLDALRNG"
     gene            13426..14562
                     /gene="hemW"
                     /locus_tag="OO357_002981"
     CDS             13426..14562
                     /gene="hemW"
                     /locus_tag="OO357_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HCR5887851.1"
                     /translation="MVKLPPLSLYIHIPWCVQKCPYCDFNSHALKGEVPHDDYVQHLL
                     CDLDNDVAYAQGREVKTIFIGGGTPSLLSGPAMQTLLDGVRARLPLAADAEITMEANP
                     GTVEADRFVDYQRAGVNRISIGVQSFSEEKLKRLGRIHGPQEAKRAAKLASGLGLRSF
                     NLDLMHGLPDQSLEEALGDLRQAIELNPPHLSWYQLTIEPNTLFGSRPPVLPDDDALW
                     DIFEQGHQLLTAAGYQQYETSAYAKPGYQCQHNLNYWRFGDYIGIGCGAHGKVTFPDG
                     RILRTTKTRHPRGFMQGRYLESQRDVEAADKPFEFFMNRFRLLEAAPRVEFIAYTGLC
                     EDVIRPQLDEAIAQGYLTECADYWQITEHGKLFLNSLLELFLAE"
     gene            14876..15839
                     /locus_tag="OO357_002982"
                     /pseudo
     CDS             14876..15839
                     /locus_tag="OO357_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231167.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            15884..16387
                     /locus_tag="OO357_002983"
     CDS             15884..16387
                     /locus_tag="OO357_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708723.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HCR5887852.1"
                     /translation="MIFNRLKLAVDRVIATFSVAVMLALVVCVVWQVFSRYVLNQPST
                     LTDELARFLMIWVGLLGAAYTVGAQRHLSIDLFALALNKRKQLLLSIVINVLILGFAG
                     SVIVTGGLKLIDKTLATSQVSAAMQIPMGYVYIILPLSGVVMMFYALCFINQSIQQLK
                     QPAQEAS"
BASE COUNT         4061 a         4220 c         4124 g         3986 t
ORIGIN      
        1 aaagcagcta gattattttt aagaatatat aaaaagaaag cgggccgatt tacgtaaata
       61 aatcgacccg ccagcggaat aatcttaatt attcatcttc aagataagta taaccgtaca
      121 aacctgcctc gaactcttca aggaactgtt gttgcagttc agcatcaaga tcggttttct
      181 tcacttgatc gcggaactgg gttaacagcg ttttcggatc gagctgtaca tattgcagca
      241 tgtccgccac ggtatcgcct tcgtcagaca gttctacttc tacgctaccg tcagggaaca
      301 cgaacacgtc aactgcttcg gtatcaccga acaggttgtg catattgcca aggatctcct
      361 gatatgcacc gaccataaag aaaccgagca tcggcggatt ctctggatcg tactccggca
      421 ttggcattgt cgtggcaata ccatcaccat caatatagtg gtcgatagca ccgtcagagt
      481 cacaggtaat atccagcagc acagcgcgac gttccggcac ttgatccagc ccttccagcg
      541 gtagaaccgg gaacaactgg tcgatccccc atgcgtccgg cattgactgg aacagcgaga
      601 aattgacgta cattttgtcc gccatacgtt cctgcagttc gtcgattatc ggacgatgcg
      661 cacggttttg cggatccagt tgcttctgca cttcatggca catgctcaga tagagttgtt
      721 ctgcccatgc acgttcttgc aggctaaagg tgccggaaga gtagccgata tgaatgtcgt
      781 gcagatccat ctggctgtcg tgtaaccatt cacgcaaaga acggcgagtt cccggttcgt
      841 gcatttcctg ccaggtttcc cacatgcttt gcagcgcacg cggcgcatct tctacgggcg
      901 cggtcggcac cgtatattcg ttacgttcca cgccgatgat attcgacacc agcacggtat
      961 ggtgcgcagt caccgcacgg cccgattcgg tgattaccgt cggatgcggc agaccatttt
     1021 cttcacacgc atcgccaatc gcccagatga tgttgttggc gtattcattg aggccatagt
     1081 ttacggagca gtcagactgc gaacgggtgc cttcataatc cacgcccaga ccgccgccga
     1141 cgtcgaagca ctgaatattg acgcccagct tgtgcagttc cacatagaaa cgcgcggatt
     1201 cacgaacgcc tgtcgcgata tcgcgaatat tcgccatctg cgaaccgagg tggaagtgca
     1261 gtagttgcag gctgtcgaga cgcccggctt cacgcagggt ttcaaccagt tgcagtacct
     1321 gagtcgcagc caggccgaac ttcgattttt ccccgccgga ggactgccat ttacccgagc
     1381 cctgcgaagc cagacgtgca cgcacgccca gacgaggaac gacattcaga cgttctgctt
     1441 catccagcac aatggcgatt tctgacatct tctcaatgac cagatagacc ttgtgcccca
     1501 tcttctcgcc aattaatgcc aggcggatat attcgcggtc tttataaccg ttgcagacga
     1561 tgacgctacg agtcatgcca gcatgtgcca gcactgccat caactcggct ttggaaccgg
     1621 cttccagacc cagcggttca cccgaatgaa tcagggactc gatcacgcgg cggtgctggt
     1681 taactttgat cggataaaca aggaagtaat cgccgttata gccgtaggat tccctcgcac
     1741 gtttgaacgc ggcgttaatg gaacgcaaac ggtgctgcag gatctgtggg aaacagaaca
     1801 gtgcaggcag acgctgaccc tgtgcttcac gagttttcac taactgcgcg agatcgacgc
     1861 gagcttccgg gacgtccggg tccgggcaca cgctgatgtg gcccagctcg ttaacgtcat
     1921 agtagttatt gccccaccag gcaatattgt aagtacgcag catcttgctg gcttcctggg
     1981 agctcattgc aacctcctgc atggagcgta gtacaccgtg ttcgcccgct gacgaaggca
     2041 aacccataga catgtcgtca gacatagcga acctcaaatt attttattaa gtgtaaaaca
     2101 gttaacgact gtcgcagcct ggaactgcga taacaaccca taagcacatg gattttccag
     2161 cagtgtatgc tgacgctccg actgcgcaac cggtttcttt ttcataacat tattaagcac
     2221 ataaccgaac gtaagtgtga cagttcggcg aaaccacgag aaaactcttg tttttacaag
     2281 agcgcccttg ttcagtcctc agtaactgta accagctctt gaatcctgag aagcgccgag
     2341 atgggtataa catcggcagg tatgcaaagc agagatgcag agtgcgggga acgagtcttc
     2401 accagaacgg tgagacaggt taagcagcag acaacggttc attatttcgt atcacctcca
     2461 cggccgcctg ttaagacgaa cccacaagcc aaaactctga tttcaacccg gctggaagtg
     2521 gcaacacgaa agaaacgtcg tgtgcttttt atttaagccg cgcgccgcgt tttatacccc
     2581 acaatggcag aaaattgcaa aagataaata cgcagaatgc cggcattgtc aggaaaaatt
     2641 tccagccacg tttttaacag aatgagacac gattcaaaaa aaagtggaaa tcgggtgaag
     2701 aattgaccta aaatagccat ccagatgtta atccatccat accgattaac actcagactg
     2761 ccagtgtttt taacctgcag agtcgtggta ggatccgcta ccacagaaaa tccacacaac
     2821 agtttgagct aaccaaattc tctttaggtg atattaaata tggcaaaaca cctttttacg
     2881 tccgagtccg tctctgaagg gcatcctgac aaaattgctg accaaatctc tgatgccgtt
     2941 ttagacgcga tcctcgaaca ggatccgaaa gcacgcgttg cttgcgaaac ctacgtaaaa
     3001 accggcatgg ttttagttgg cggcgaaatc accaccagcg cctgggtaga catcgaagag
     3061 atcacccgta acaccgttcg cgaaattggc tatgtgcatt ccgacatggg ctttgacgct
     3121 aactcctgtg cggttctgag cgctatcggc aaacagtctc ctgacatcaa ccaaggcgtt
     3181 gaccgtgccg atccgctgga acagggcgcg ggtgaccagg gtctgatgtt tggctacgca
     3241 actaatgaaa ccgacgtgct gatgccagca cctatcacct atgcacaccg tctggtacag
     3301 cgtcaggctg aagtgcgtaa aaacggcact ctgccgtggc tgcgcccgga cgcgaaaagc
     3361 caggtgactt tccagtatga cgacggcaaa atcgttggta ttgatgctgt cgtgctttcc
     3421 actcagcact ctgaagagat cgaccagaaa tcgctgcaag aagcggtaat ggaagagatc
     3481 atcaagccaa ttctgcccgc tgaatggctg acttctgcca ccaaattctt catcaacccg
     3541 accggtcgtt tcgttatcgg tggcccaatg ggtgactgcg gtctgactgg ccgtaaaatt
     3601 atcgttgata cctacggcgg catggcgcgt cacggtggcg gtgcattctc tggtaaagat
     3661 ccatcaaaag tggaccgttc cgcagcctac gcagcacgtt atgtcgcgaa aaacatcgtt
     3721 gctgctggcc tggccgatcg ttgtgaaatt caggtttcct acgcaatcgg cgtggctgaa
     3781 ccgacctcca tcatggtaga aactttcggt actgagaaag tgccttctga acaactgact
     3841 ctgctggtac gtgagttctt cgacctgcgc ccatacggac tgattcagat gctggatctg
     3901 ctgcacccga tctacaaaga aaccgcagca tacggtcact ttggtcgtga acatttcccg
     3961 tgggaaaaaa ccgacaaagc gcagctgctg cgcgatgctg ccggtctgaa gtaatctttc
     4021 ttcacctgcg ttcaaaggcc agcctcgcgc tggccttttt cttttacctc accgcggtgg
     4081 ataggcgttc acgccgcatc cggcaaaaaa actgcccgca caataacatc attcttcctg
     4141 atcacgtttc acggcaaatt accatcacaa ctgaaaccga ttacaccaac cacaccagca
     4201 aaatatttgt aatattttca tattattatt cagctttcac agttgttaca tttcttttca
     4261 gtaaagtctt aattgcagat gacagcgttt aatctatgat gatataactc aattattttc
     4321 atgcccttaa atcataacta agataaatgt tagtgtaagc gattacactg atgtgatttg
     4381 cttcacatct ttttacgtcg tactcaccta tcttaattca caataaaaaa taactatatt
     4441 ggagggcatc atgcctgacg ctaaaaaaca ggggcggtca aacaaggcaa tgacgttttt
     4501 cgtctgcttc cttgccgctc tggcgggatt actctttggc ctggatatcg gtgtaattgc
     4561 tggcgcactg ccgtttattg cagatgaatt ccagataact tcgcacacgc aagaatgggt
     4621 cgtaagctcc atgatgttcg gtgcggcagt cggtgcggtg ggcatcggct ggctctcctt
     4681 taaactcggg cgcaaaaaga gcctgatgat cggcgcaatc ctgtttgttg ccggttcact
     4741 gttctctgcg gctgcgccaa acgttgaagt actgattctt tcgcgcgttc tgctggggct
     4801 ggcggtgggt gtggcctctt ataccgcacc actgtacctg tcagaaattg cgccggaaaa
     4861 aattcgcggc agtatgatct cgatgtatca gttgatgatc actatcggga tcctcggtgc
     4921 ttatctttct gataccgcct tcagctacac cggtgcatgg cgctggatgc tgggtgtgat
     4981 tatcatcccg gcaattttgc tgctgattgg tgtcttcttc ctgccagaca gcccacgttg
     5041 gtttgccgcc aaacgccgtt ttgttgatgc cgaacgcgtg ctgctacgcc tgcgtgacac
     5101 cagcgcggaa gcgaaacgcg aactggatga aatccgtgaa agtttgcagg ttaaacagag
     5161 tggctgggcg ctgtttaaag agaatagcaa cttccgccgc gcggtgttcc ttggcgtact
     5221 gttacaggta atgcagcaat tcaccgggat gaacgtcatc atgtattacg cgccgaaaat
     5281 cttcgaactg gcgggttata ccaacaccac cgagcaaatg tgggggacag tgattgtcgg
     5341 cctgaccaac gtacttgcca cctttatcgc aatcggcctt gttgaccgct ggggacgtaa
     5401 accaacgcta acgctgggct tcctggtgat ggctgctggc atgggcgtac tcggtacaat
     5461 gatgcatatc ggtatccact ctccgtcggc gcagtatttc gccatcgcca tgctgctgat
     5521 gtttattgtc ggttttgcca tgagtgccgg tccgctgatt tgggtactgt gctccgaaat
     5581 tcagccgctg aaaggccgcg attttggcat cacctgctcc accgccacca actggattgc
     5641 caacatgatc gttggcgcaa cgttcctgac catgctcaac acgctgggta acgccaacac
     5701 cttctgggtg tatgcggctc tgaacgtact gtttatcctg ctgacattgt ggctggtacc
     5761 ggaaaccaaa cacgtttcgc tggaacatat tgaacgtaat ctgatgaaag gtcgtaaact
     5821 gcgcgaaatc ggcgctcacg attaatctcc tcaggcttcc tcccgtcgcg gaggaagccg
     5881 cctcttgcag tcatcttttc ttcgctctat cctctgccgc tatgaaaaca tcccgtctcc
     5941 ctatcgccat ccaacaggcc gttatgcgcc gcctgcggga aaaactcgcc caggccaacc
     6001 tgaagctagg gcgtaactac ccggagccaa aactctctta cacccagcgc ggaacctccg
     6061 ccggaacagc ctggctggaa agctatgaaa tccgcctcaa tcccgttttg ctgttggaaa
     6121 acagtgaagc ttttattgaa gaagtggtac cgcacgaact ggcacatttg ctggtatgga
     6181 aacatttcgg ccgcgtagcg ccacatggca aagagtggaa gtggatgatg gaaagcgtgc
     6241 tgggcgttcc cgcccgtcgt acgcatcagt tcgaactgca atccgtgcgt cgcaacacct
     6301 tcccctaccg ctgtaagtgc caggaacatc agcttaccgt acgccgccat aatcgcgtag
     6361 ttcgtggcga ggccatctat cgctgtgttc actgcggtga acagctggtt gcgaaataac
     6421 cacctgaact atcaggaact ttcctgatct ggctgattgc ataccaaaac aactttcgct
     6481 acgttgctgg ctcgttttaa cacggagtaa gtgatgtacc gttatttgtc tattgctgcg
     6541 gtggtactga gcgcagcatt ttccggcccg acgttggccg aaggtatcaa tagtttttct
     6601 caggcgaaag ccgcggcggt aaaagtccac gctgatgcgc ccggtacgtt ttattgcgga
     6661 tgtaaaatta actggcaggg caaaaaaggc gttgttgatc tgcaatcgtg cggctatcag
     6721 gtgcgcaaaa atgaaaaccg cgccagccgc gtagagtggg aacacgtcgt tcccgcctgg
     6781 caattcggtc accagcgcca gtgctggcag gacggtggac gtaaaaactg cgctaaagat
     6841 ccggtctatc gcaagatgga aagcgatatg cataacctgc agccgtcagt cggcgaggtg
     6901 aatggcgatc gcggcaattt tatgtacagc cagtggaatg gcggcgaagg ccagtacggt
     6961 caatgcgcca tgaaggtcga tttcaaagaa aaagctgccg aaccaccagc gcgtgcacgc
     7021 ggtgccattg cgcgcaccta cttctatatg cgcgaccaat acaacctgac actctctcgc
     7081 cagcaaacgc agctgttcaa cgcatggaac aagatgtatc cggttaccga ctgggagtgc
     7141 gagcgcgatg aacgcatcgc gaaggtgcag ggcaatcata acccgtatgt gcaacgcgct
     7201 tgccaggcgc gaaagagcta acctacacta gcgggattct ttttgttaac ccctacccca
     7261 cgcgtacaac cgcgtgggga gacgacgcgg atttttaact atgcgtatcc cccgcattta
     7321 tcatcctgaa ccactgacca gccattctca catcgcgctt tgcgaagatg ccgccaacca
     7381 tatcgggcgc gtactgcgca tggggccggg gcaggcgttg caattgtttg acggtagcaa
     7441 ccaggtcttt gacgccgaaa ttaccagcgc cagcaaaaaa agcgtggaag tgaaggtgct
     7501 ggaaggccag atcgacgatc gcgaatctcc gctgcatatt cacctcggtc aggtgatgtc
     7561 gcgtggtgaa aaaatggaat ttactatcca gaaatcgatc gaactcggtg taagcctcat
     7621 tacgccactt ttttctgagc gctgcggcgt taaactggat agtgaacgtc tgaacaagaa
     7681 gcttcagcag tggcagaaga ttgcaattgc tgcctgtgag cagtgtggtc gtaaccgggt
     7741 gccggaaatc cgtccagcga tggatctgga agcctggtgt gcagagcagg atgaaggact
     7801 gaaactgaat cttcacccgc gcgccagtaa cagcatcaat acgttgcctt taccggttga
     7861 acgcgtccgc ctgctgattg gcccggaagg cggtttatcg gcagatgaaa ttgccatgac
     7921 tgcccgctat caatttactg atatcctgtt gggacctcgc gttttgcgta cagagacaac
     7981 tgcgctcacc gccattaccg cgctacaagt acgatttggc gatttgggct aacggagaag
     8041 aataatgatc aagctcggca tcgtgatgga ccccatcgca aacatcaaca tcaagaaaga
     8101 ttccagtttc gctatgttgc tggaagcaca gcgtcgtggt tacgaacttc actatatgga
     8161 gatgggcgat ctgtatctga tcaatggcga agcccgcgcc catacccgca cgctgaacgt
     8221 gaagcagaac tacgaagagt ggttttcgtt cgtcggtgaa caggatctgc cgctggccga
     8281 tctcgatgtg atcctgatgc gtaaagaccc gccgtttgat accgagttta tctacgcgac
     8341 ctatattctg gaacgtgccg aagagaaagg gacgctgatc gttaacaagc cgcagagcct
     8401 gcgcgactgt aacgagaaac tgtttaccgc ctggttctct gacttaacgc cagaaacgct
     8461 ggttacgcgc aataaagcgc agctaaaagc gttctgggag aaacacagcg acatcattct
     8521 taagccgctg gacggtatgg gcggcgcgtc gattttccgc gtgaaagaag gcgatccaaa
     8581 cctcggcgtg attgccgaaa ccctgactga gcatggcact tgctactgca tggcgcaaaa
     8641 ttacctgcca gccattaaag atggcgacaa acgcgtgctg gtggtggatg gcgagccggt
     8701 accgtactgc ctggcgcgta ttccgcaggg gggcgaaacc cgtggcaatc tggctgccgg
     8761 tggtcgcggt gaacctcgtc cgctgacgga aagtgactgg aaaatcgccc gtcagatcgg
     8821 gccgacgctg aaagaaaaag ggctgatttt tgttggtctg gatatcatcg gcgaccgtct
     8881 gactgaaatt aacgtcacca gcccaacctg tattcgtgag attgaagcag agtttccggt
     8941 gtcgatcacc ggaatgttaa tggatgccat cgaagcacgt ttacagcagc agtaacccac
     9001 cttagcgaga aggatctcgt tgagactctg agtgacagcg cccttctttc cacgcatact
     9061 gggcgctgtt gcttttttga accaggaaac agaacctctg acaatgaatt tacagcatca
     9121 ctttcttatt gccatgcctg ctctccagga tccgattttc cgtcgttccg tggtctacat
     9181 ttgcgaacat aataccaatg gtgcaatggg gatcatcgtc aacaagccgc tggaaaatct
     9241 caaaattgaa gggattctgg aaaagctgaa gatcacgccg gagccgcgtg atgaatcaat
     9301 ccgtctggat aaaccggtta tgctcggcgg cccactggct gaagatcgcg ggtttatttt
     9361 gcatactccg ccctccaatt ttgcttccag cattcgcatt tcagacaaca cggtgatgac
     9421 cacttcccgc gatgtgctgg aaacgctcgg caccgataaa caaccgtctg acgtattggt
     9481 ggctctgggc tatgcctcct gggaaaaagg tcaactggaa caagaaattc tcgataacgc
     9541 gtggctaacg gccccggcgg atctgaatat tctgttcaaa acgccgattg ccgaccgctg
     9601 gcgcgaggcg gcaaaactga ttggtgtgga tattctcacc atgcctggtg tggcaggaca
     9661 cgcctgatga gtggaacctt actcgccttc gacttcggca ccaaaagcat tggcgtagcg
     9721 gtcggccaac gcattaccgg caccgctcgc cctttgcctg caattaaagc gcaggacggt
     9781 acgccggact ggaacctcat cgaacgttta ctgaaagagt ggcagcctga cgaaatcatc
     9841 gtcggtttgc cgctgaatat ggacggcacc gaacagccgt taaccgccag agcgcgcaaa
     9901 tttgccaacc gtattcatgg tcgtttcggt gttgaagtaa agctccatga cgagcgtctt
     9961 agcactgtgg aagcccgttc cggtcttttt gaacagggcg gctatcgggc gctcaacaaa
    10021 ggcaaagttg actctgcctc tgcggttatt attctcgaaa gctatttcga gcagggatat
    10081 taaggcgatc taaaagcctg gcggatagtc gttaccgcca tcttgccgga tgcggcgtga
    10141 acgccttatc cagcacacat ctggcagcgg ctgtaggtct gataagacgc gttagcgtcg
    10201 catcagacat ttattgcctt tataggccct ataaacttgc gcatcgggca tggcaacgtc
    10261 acaaacgccc ttcccccacc cgctgctgat aactctgctg aaacgttaac atccccacct
    10321 tctgcccggt ttgaataaca tgcggtaact ggtgggtttt cccttcgcga atcaaattcc
    10381 ccaccgcggg agtgttaatc agtaattcaa acagcgccac gcgtccttcc tgtttatcca
    10441 cttccagctt ttgtgacagc actgcccgta aactacccgc aagttgatta cgtacgggat
    10501 ctttttcctg cgccggaaat gaatccacca gtcgctcaac tgcctgcgcg gcaccacgtg
    10561 tatgtaaggt tgccagcacc agatgcccgg tttctgccgc cgtcagcgcc agacggattg
    10621 tctcactgtc acgtagctct ccgagcaaaa tcacatcagg atcttcccgc aatgcagccc
    10681 gcaaccccga tgcgaacgtc atacagtgca aaccaatttc ccgctgctgg atcaaacatc
    10741 gctggctggc atagagatat tccacaggat cttccagcgt cagaatatgc gcatcggcat
    10801 gttgattaag atagccaacc atcgccgcca gcgtggtaga tttgccactc cccgtagccc
    10861 ccgtcaccag aatcaggcca ttctcgctct tgagtaattc cggcaatacc ggtggtgcac
    10921 caagctgttc gagctgtgga cagtgcgaag gtaacaaccg taatgccaga gaaatacctt
    10981 gccgttgcgc gaacgcactg ccacgcaacc gctggttttc cgccagcgac acggcaaaat
    11041 ccagctggcc attttccagc aatattgccc gctgatcgtc atccagccac tcccgcagta
    11101 gctcttcgac gtccggcgca tcaaacggcg cagcttccat tctgccacga atgcgccatc
    11161 gtgcgggcca ggcgctgcac aggtgtagat ccgagacgtt atgctttaca ctaagggcca
    11221 caatttcttc catattcata ctaagatcct cggaaaatga acgatattgc gcataacctg
    11281 gcacaggtcc gggacaaaat ctcagcggct gcaacgcgtt gcggccgttc tccagaagaa
    11341 attacgctgc ttgcagtcag taaaacaaaa cctgcgagcg ccatcgcaga agccattgat
    11401 gccgggcagc gtcaatttgg tgaaaactac gttcaggaag gggtagataa aattcgccac
    11461 tttcaggaac tgggcgtaac aggattagaa tggcatttta ttggcccgtt gcagtctaat
    11521 aaaagccgcc tagtggcaga gcatttcgac tggtgtcata ccatcgaccg tttgcgcatt
    11581 gctacccgcc tcaacgagca gcgcccggca gaacttcccc ctcttaacgt actgattcaa
    11641 attaatatta gtgatgaaaa cagtaagtcc gggattcaac tggcagaact ggacgagctg
    11701 gcagcggcgg tcgctgaact accgcgttta cgtctgcgcg ggctgatggc aatccctgcg
    11761 cttgagtcag aatatgtaag gcagtttgaa gttgcacgcc aaatggctgt agcatttgcc
    11821 ggactgaaaa cgcgctaccc gcatatcgac acgctctctc tgggaatgtc ggacgatatg
    11881 gaagccgcca ttgcggcagg tagcacgatg gttcgtatcg gcactgcaat ttttggtgcg
    11941 cgtgattact ctaaaaaata aggaattaaa ggaacgccat gaatacgttg actttcctgc
    12001 tttcaacggt cattgagctg tatacaatgg tgctgttatt acgcatctgg atgcagtgtg
    12061 ctcattgtga tttttacacc cccttctcac agtttgtagt gaaggtagcg cagccaatta
    12121 tcgggccact gcgccgcgtt attccggcaa tgggtcccat tgacagcgcc tcgctgctgg
    12181 ttgcctatat tctcagtttt atcaaagcca ttgtgctgtt tgaagtggtg accttcctgc
    12241 caatcatctg gattgccggt ttactgattc tgctgaaaac catcggcttg ctgattttct
    12301 gggtcctgct ggtgatggcg attatgagct gggtaagcca gggtcgtagc ccgattgaat
    12361 acgtgctgat tcagctggcc gatccgctgc tgcgcccgat tcgccgcttg ctaccggcaa
    12421 tgggtgggat tgatttctcg ccgatgatcc tcgttctgct gctgtatgcc atcaatatgg
    12481 gtgtcgcaga agtattacag gcaaccggaa acatgctgct gccggggctg tggatggcgt
    12541 tatgagtgcc gtaacagtta atgatgacgg tctggtttta cggctctata ttcagccgaa
    12601 agccagccgt gattctattg tcggtttaca tggcgacgaa gttaaagtcg ccattaccgc
    12661 gccgccggtt gacggccagg ccaacagtca tctggtgaag tttctcggta agcaattccg
    12721 ggttgccaaa agccaggtgg tgattgaaaa aggcgaactt ggccgccaca aacaaattaa
    12781 aatcattaat ccgcaacaaa tcccgccaga aatcgcggcg ttaattaatt aggtatccta
    12841 tgcaaaaagt tgtcctcgca accggcaatg ccggtaaagt gcgtgagctg gcgtcgctgc
    12901 ttagcgactt tggtcttgat atcgtggccc aaacagacct cggcgttgat tccgctgaag
    12961 aaaccggcct aacctttatc gaaaacgcga ttctgaaagc gcgccatgcg gcaaaagtga
    13021 ccggtttacc ggcaattgcc gacgactctg gtctggcggt agatgcgctg ggtggcgcgc
    13081 cgggtattta ctccgcgcgt tattccggtg aagacgcgac cgatcaaaag aatctgcaaa
    13141 aactgttgga aacactgaaa gacgtaccgg acgaccaacg tcaggcgcgt ttccactgcg
    13201 tgctggtata tctgcgtcac gcggaagatc cgactccgct ggtatgccac ggcagctggc
    13261 cgggcgtgat tactcgcgaa ccagcgggca ctggtggctt tggttatgat ccaatcttct
    13321 tcgtaccttc cgaagggaaa accgccgccg aactgacccg cgaagaaaag agcgccattt
    13381 cccaccgtgg tcaggcgttg aaactgctgc tggacgcttt acgtaatggt taaattaccg
    13441 ccgctgagtc tctacattca catcccgtgg tgcgtgcaga aatgcccgta ctgcgatttc
    13501 aactctcacg cgttgaaagg agaagtgccg cacgacgatt atgttcagca tctgctttgc
    13561 gatctggaca atgatgtggc ttacgctcag ggccgtgaag taaagacaat ctttattggc
    13621 ggtggtacgc cgagcctgct ttccggcccg gcgatgcaaa cgctgctaga cggcgtgcgt
    13681 gcgcgtttgc cgctggcagc ggatgcagaa attactatgg aagcgaaccc aggcacggta
    13741 gaagcggatc gctttgtcga ttatcagcgt gctggtgtaa accgcatctc tattggtgtg
    13801 cagagtttta gcgaagaaaa gctgaaacga cttgggcgta ttcatggccc gcaagaagcg
    13861 aaacgagcgg caaagctggc gagcggttta gggttacgta gctttaacct tgatttgatg
    13921 catggactac cggatcaatc actggaagag gcgcttggcg atctacgcca ggccattgaa
    13981 ctgaatccgc cgcatctttc ctggtatcaa ctgactatcg aacctaatac gctgtttggc
    14041 tcgcgaccgc cggtgctgcc ggacgatgac gcgctgtggg atattttcga acaggggcat
    14101 cagttattaa ccgcagcggg ttatcagcaa tatgaaactt ccgcttacgc caaacccggt
    14161 tatcagtgcc agcacaatct caactactgg cgctttggtg actacatcgg tattggctgc
    14221 ggcgcgcacg gcaaagtgac cttcccggat gggcgcattc tgcgtaccac taaaacgcgt
    14281 catcctcgtg gttttatgca aggaaggtat ctggaaagcc agcgtgatgt cgaagccgca
    14341 gataagccgt ttgagttctt tatgaatcgc ttccgtctgc tggaggccgc gccgcgcgtg
    14401 gagtttattg cgtataccgg gctttgcgaa gatgtgattc gcccacagtt agacgaggcg
    14461 attgcccagg gttatctcac cgaatgtgcg gattactggc agataacgga acatggtaaa
    14521 ttattcttaa actcacttct tgaattattc ctcgctgagt aatcattaat gcgcctgcgg
    14581 gcgcattaat cacttctttt atccccccgt taattaacct cggcagaaat atatttctta
    14641 ctgaataaat atcgacgaca tttattatat atatttagtg aatatttact aatcattcat
    14701 tgattataaa tatgtacaac ctggcgcaca aatttaatat ttaatttctg actttatttc
    14761 aagttgtgat aaacatagac aagttgtcag acaggttaac caatttgtcg cacaaatcac
    14821 tgataagcac aattagatat caaccaattt ccatccttca acagggagtt tttttatgaa
    14881 aatgaggtca tttacccgct cattagtctg cgcatccttg ctggctctgg ttagcactgg
    14941 cgtaaacgcc gcagaaaaag tggcgttaaa gttagcacac aacctggaaa gaagtcatgt
    15001 tgtacatcag agttttgaag agctggcgaa agaggtaaaa cagctgtcaa aaggtaatat
    15061 ggtgatccgt atttatccca gcagccagat gggtaatgcg cgagaaacta tggaattgtt
    15121 gcaaaatggc gcactggata tgaccaaagg ttccgccagt gatctggagt cttttgataa
    15181 tatttatgct atctataatt tgccgttctt attaagttgt ttttggtgaa gttggtaaag
    15241 aaattatgga ctcaacaaaa gacaaaggct tttttgcttt atcagcttat gtagcgggta
    15301 ctcgcagttt ctatgcgaaa aaaccgatta caaagccaga agatcttaaa ggactgaaaa
    15361 ttcgtgttca gccaagtccg accaccatca aaatgattga actgatgggt ggttcaccca
    15421 ctccaatttc atttggcgaa gtttatactg cgatgcagca aggtgtagta gatggcgcag
    15481 aaaataacgt gccttcctgg atgcagactc gacatattga aattgccaaa gttttttctg
    15541 aagatgaaca cgcctcaatt cctgactttt tggttatctc caccaaaaca tggaataaat
    15601 taaccccaga acagcaacaa atcctcgaaa cagcggcgaa gaaatccgaa gcttatcaac
    15661 aaaaattgtg ggaaaaaatt gacgctgaca cacgtgctca ggcgaaagct atggggggtg
    15721 aaattgtaaa agtggataaa gcgccattcc gtaaagccgt gcaacctctg tttgacgact
    15781 tcaaaaaaga tccaaaacaa gctgctttgt tagagaaatt cgacaacgct gctcaataat
    15841 attccgggcc agcctctggc cctgattttc caggatgctt acaatgatat ttaaccgcct
    15901 gaaacttgcg gtagaccgcg tcattgccac gttttctgtc gccgtaatgc ttgctctggt
    15961 tgtatgcgta gtctggcaag tatttagccg ctacgtactt aaccagccaa gtacattaac
    16021 cgatgagctg gcacgctttt tgatgatctg ggtaggatta ttaggtgctg catataccgt
    16081 cggcgcgcag cgacatctct ctattgattt atttgctctc gccctgaata agcgcaaaca
    16141 attacttctt agtatagtca ttaacgttct cattttaggt tttgccggtt cggtcattgt
    16201 gactggtggc ctgaaattaa ttgataaaac actggcaaca tcacaggtat ctgccgctat
    16261 gcaaataccg atgggatacg tatatatcat tctgccatta agtggagtgg taatgatgtt
    16321 ttatgccttg tgttttatta atcaaagcat tcaacaactg aaacaacctg cgcaggaggc
    16381 cagttaatgg a
//
LOCUS       DAJTRC010000084        15930 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.084, whole genome shotgun sequence.
ACCESSION   DAJTRC010000084 DAJTRC010000000
VERSION     DAJTRC010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15930)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15930)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15930
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.084"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            446..1504
                     /gene="citC"
                     /locus_tag="OO357_002984"
     CDS             446..1504
                     /gene="citC"
                     /locus_tag="OO357_002984"
                     /EC_number="6.2.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415151.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="[citrate (pro-3S)-lyase] ligase"
                     /protein_id="HCR5887853.1"
                     /translation="MFGNDIFTRVKRSENKKMAEIAQFLHENDLSVDTTVEVFITVTR
                     DEKLIACGGIAGNIIKCVAISESVRGEGLALTLATELINLAYERHSTHLFIYTKTEYE
                     ALFRQCGFSTLTCVPGVMVLMENSATRLKRYAESLKKFRHPGNKIGCIVMNANPFTNG
                     HRYLIQQAAAQCDWLHLFLVKEDSSRFPYEDRLDLVLKGTADIPRLTVHRGSEYIISR
                     ATFPCYFIKEQSVINHCYTEIDLKIFRQYLAPALGVTHRFVGTEPFCRVTAQYNQDMR
                     YWLETPTISAAPIELVEIERLRYQEMPISASRVRQLLAKNDLTAIAPLVPAVTLHYLQ
                     NLLEHSRQDAAARQKPPA"
     gene            1519..1815
                     /gene="citD"
                     /locus_tag="OO357_002985"
     CDS             1519..1815
                     /gene="citD"
                     /locus_tag="OO357_002985"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase acyl carrier protein"
                     /protein_id="HCR5887854.1"
                     /translation="MKINQPAVAGTLESGDVMIRIAPLDTQDIDLQINSSVEKQFGDA
                     IRTTILDVLARYNVRGVQLNVDDKGALDCILHARLEALLARASGIPALPWEDCQ"
     gene            1812..2720
                     /gene="citE"
                     /locus_tag="OO357_002986"
     CDS             1812..2720
                     /gene="citE"
                     /locus_tag="OO357_002986"
                     /EC_number="4.1.3.34"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706470.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (pro-3S)-lyase subunit beta"
                     /protein_id="HCR5887855.1"
                     /translation="MISASLQQRKTRTRRSMLFVPGANAAMVSNSFIYPADALMFDLE
                     DSVALREKDTARRMVYHALQHPLYRDIETIVRVNALDSEWGVNDLEAVVRGGADVVRL
                     PKTDTAQDVLDIEKEILRIEKACGREPGSTGLLAAIESPLGITRAVEIAHASERLIGI
                     ALGAEDYVRNLRTERSPEGTELLFARCSILQAARSAGIQAFDTVYSDANNEAGFLQEA
                     AHIKQLGFDGKSLINPRQIDLLHNLYAPTQKEVDHAHRVVEAAEAAAREGLGVVSLNG
                     KMVDGPVIDRARLVLSRAELSGIREE"
     gene            2731..4263
                     /gene="citF"
                     /locus_tag="OO357_002987"
     CDS             2731..4263
                     /gene="citF"
                     /locus_tag="OO357_002987"
                     /EC_number="2.8.3.10"
                     /EC_number="4.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase subunit alpha"
                     /protein_id="HCR5887856.1"
                     /translation="MTQKIEQSQRQERVAAWNRRTECDLAAFQNSPKQTYQAEKARDR
                     KLCANLEEAIRRSGLQDGMTVSFHHAFRGGDLTVNMVMDVIAKMGFKNLTLASSSLSD
                     CHAPLVEHIRQGVVTRIYTSGLRGPLAEEISRGLLAEPVQIHSHGGRVHLVQSGELNI
                     DVAFLGVPSCDEFGNANGYTGKACCGSLGYAMVDADNAKQVVMLTEELLPYPHNPASI
                     EQDQVDLIVKVNRVGDAAKIGAGTTRMTTNPRELLIARSAADVIVNSGYFKEGFSMQT
                     GTGGASLAVTRFLEDKMRSRDIRADFALGGITATMVDLHEKGLIRKLLDVQSFDSHAA
                     QSLARNPNHIEISANQYANWGSKGASVDRLDVVVLSALEIDRQFNVNVLTGSDGVLRG
                     ASGGHCDTAIASALSIIVAPLVRGRIPTLVDNVLTCITPGSSVDILVTDHGIAVNPAR
                     PELAERLQEAGIKVVSIEWLRERARLLTGEPQPIEFTDRVVAVVRYRDGSVIDVVHQV
                     KE"
     gene            4267..4817
                     /gene="citX"
                     /locus_tag="OO357_002988"
                     /pseudo
     CDS             4267..4817
                     /gene="citX"
                     /locus_tag="OO357_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308680.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate lyase holo-[acyl-carrier protein]
                     synthase"
     gene            4792..5670
                     /gene="citG"
                     /locus_tag="OO357_002989"
     CDS             4792..5670
                     /gene="citG"
                     /locus_tag="OO357_002989"
                     /EC_number="2.4.2.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triphosphoribosyl-dephospho-CoA synthase CitG"
                     /protein_id="HCR5887857.1"
                     /translation="MSMPATSTKTTKLATSLIDEYALLGWRAMLTEVNLSPKPGLVDR
                     INCGAHKDMALEDFHRSALAIQGWLPRFIEFGACSAEMAPEAVLHGLRPIGMACEGDM
                     FRATAGVNTHKGSIFSLGLLCAAIGRLLQLNQPVTPTTVCSTAASFCRGLTDRELRTN
                     NSRLTAGQRLYQQLGLTGARGEAEAGYPLVINHALPHYLTLLDQGLDPELALLDTLLL
                     LMATNGDTNVASRGGEGGLRWLQREAQTLLQKGGIRTPTDLDYLRQFDRECIERNLSP
                     GGSADLLILTCFLAQI"
     gene            5721..7183
                     /gene="citT"
                     /locus_tag="OO357_002990"
                     /pseudo
     CDS             5721..7183
                     /gene="citT"
                     /locus_tag="OO357_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308678.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/succinate antiporter CitT"
     gene            7297..8103
                     /gene="rna"
                     /locus_tag="OO357_002991"
     CDS             7297..8103
                     /gene="rna"
                     /locus_tag="OO357_002991"
                     /EC_number="4.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease I"
                     /protein_id="HCR5887858.1"
                     /translation="MKAFCRNAALLAVSLLPLSSANAVALQAKQYGDFDRYVLALSWQ
                     TGFCQSQHDRNRNERDECRLQTETTNKTDFLTVHGLWPGLPKSVAARGVDERRWMRFG
                     CATRPIPNLPEARASRMCSSPETGLSLETAAKLSEVMPGAGGRSCLERYEYAKHGACF
                     GFDPDAYFGTMVRLNQEIKESEAGKFLADNYGKTVSRRDFDAAFAKSWGKENVKAVKL
                     TCQGNPAYLTEIQISIKADAINAPLSANSFLPQPHPGNCGKTFVIDKAGY"
     gene            8332..8742
                     /gene="rnk"
                     /locus_tag="OO357_002992"
     CDS             8332..8742
                     /gene="rnk"
                     /locus_tag="OO357_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside diphosphate kinase regulator"
                     /protein_id="HCR5887859.1"
                     /translation="MSRPTIIINDLDAERIDILLEQPAYAGLPIADALNAELDRAQMC
                     SPEEMPHDVVTMNSRVKFRNLSDGEVRVRTLVYPAKMTDSNTQLSVMAPVGAALLGLR
                     VGDSIHWELPGGVATHLEVLELEYQPEAAGDYLL"
     gene            complement(8973..10211)
                     /locus_tag="OO357_002993"
     CDS             complement(8973..10211)
                     /locus_tag="OO357_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent formaldehyde
                     dehydrogenase"
                     /protein_id="HCR5887860.1"
                     /translation="MKALTYHGPHHVQVENVPDPGIEQADDIILRITVTAICGSDLHL
                     YRGKIPQVKHGDIFGHEFMGEVVETGKDVKNLQKGDRVVIPFVIACGDCFFCRLQQYA
                     ACENTNAGKGAALNKKQIPAPAALFGYSHLYGGVPGGQAEYVRVPKGNVGPFKVPPLL
                     SDDKALFLSDILPTAWQAAKNVQIQQGSSVAVYGAGPVGLLTIACARLLGAEQIFVVD
                     HHPYRLRFAADRYGAIPINFDEDSDPAQSIIEQTAGHRGVDAVIDAVGFEAKGSTTET
                     VLTNLKLEGSSGKALRQCIAAVRRGGIVSVPGVYAGFIHGFLFGDAFDKGLTFKMGQT
                     HVHAWLGELLPLIEKGLLKPEEIVTHYMPFEEAARGYEIFEKREEECRKVILVPGAQS
                     AEAAQKAVSGLVNAMPGGTI"
     gene            10432..10860
                     /gene="uspG"
                     /locus_tag="OO357_002994"
     CDS             10432..10860
                     /gene="uspG"
                     /locus_tag="OO357_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspG"
                     /protein_id="HCR5887861.1"
                     /translation="MYKAIIMPVDVFEMELSDKAVRHAEFLAQDDGVIHLLHVLPGSA
                     SLSLHRFAADVRRFEEHLQHEAEERLQTMVSHFTIDPSRIKQHVRFGSVRDEVNELAE
                     ELGADVVVIGSRNPSISTHLLGSNASSVIRHANLPVLVVR"
     gene            complement(10981..12546)
                     /gene="ahpF"
                     /locus_tag="OO357_002995"
     CDS             complement(10981..12546)
                     /gene="ahpF"
                     /locus_tag="OO357_002995"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017455864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HCR5887862.1"
                     /translation="MLDTNMKTQLKAYLEKLTKPVELIATLDDSAKSAEIKELLAEIA
                     ELSDKVTFKEDNSLPVRKPSFLITNPGSNQGPRFAGSPLGHEFTSLVLALLWTGGHPS
                     KEAQSLLEQIRHIDGDFEFETYYSLSCHNCPDVVQALNLMSVLNPRIKHTAIDGGTFQ
                     NEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAENRAAEELNKRDAYDVLIV
                     GSGPAGAAAAIYSARKGIRTGLMGERFGGQILDTVDIENYISVPKTEGQKLAGALKVH
                     VDEYDVDVIDSQSASKLIPAAVEGGLHQIETASGAVLKARSIIVATGAKWRNMNVPGE
                     DQYRTKGVTYCPHCDGPLFKGKRVAVIGGGNSGVEAAIDLAGIVEHVTLLEFAPEMKA
                     DQVLQDKLRSLKNVDIILNAQTTEVKGDGSKVVGLEYRDRVSGNIHNIELAGIFVQIG
                     LLPNTNWLEGAVERNRMGEIIIDAKCETNVKGVFAAGDCTTVPYKQIIIATGEGAKAS
                     LSAFDYLIRTKTA"
     gene            complement(12791..13354)
                     /gene="ahpC"
                     /locus_tag="OO357_002996"
     CDS             complement(12791..13354)
                     /gene="ahpC"
                     /locus_tag="OO357_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003858764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit C"
                     /protein_id="HCR5887863.1"
                     /translation="MSLINTKIKPFKNQAFKNGEFIEITEKDTEGRWSVFFFYPADFT
                     FVCPTELGDVADHYEELQKLGVDVYAVSTDTHFTHKAWHSSSETIAKIKYAMIGDPTG
                     ALTRNFDNMREDEGLADRATFVVDPQGIIQAIEVTAEGIGRDASDLLRKIKAAQYVAS
                     HPGEVCPAKWKEGEATLAPSLDLVGKI"
     gene            13726..14472
                     /gene="dsbG"
                     /locus_tag="OO357_002997"
     CDS             13726..14472
                     /gene="dsbG"
                     /locus_tag="OO357_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415137.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiol:disulfide interchange protein DsbG"
                     /protein_id="HCR5887864.1"
                     /translation="MLKKILLLALLPAIAFAEELPSPVKAIEKQGITIIKTFDAPGGM
                     KGYLGKYQDMGVTIYLTPDGKHAISGYMYNEKGENLSNTLIEKEIYAPAGREMWQRME
                     QSHWLLDGKKDAPVIVYVFSDPFCPYCKQFWQQARPWVDSGKVQLRTLLVGVIKPESP
                     ATAAAILASKDPAKTWQQYEASGCKLKLNVPANVSTEQMKVLSDNEKLMDDLGANVTP
                     AIYYMSKENTLQQAVGLPDQKTLNIIMGNK"
     gene            14682..15584
                     /locus_tag="OO357_002998"
     CDS             14682..15584
                     /locus_tag="OO357_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5887865.1"
                     /translation="MANLYDLKKFDLNLLVIFECIYQHLSISKAAESLYITPSAVSQS
                     LQRLRAQFNDPLFIRSGKGIAPTTTGLNLHHHLEKNLKGLEQTINIVNKSELKKNFII
                     YGPQLISCSNNSMLIRCLRQDASVEIECHDILMSAENAEELLVHRKADLVITQMPVIS
                     RSVICMPLHTIRNTLICSNKHPRITDNSTYEQIMAEEFTQLIAKSAGVDDIQMEIDEK
                     FMNRKISFRGSSLLTIINSIAVTDLLGIVPYELYNYHRDFLNLKEIKLEHPLPSIKLY
                     ISYNKSSLNNLVFSRFIDRLNESF"
     gene            15731..15930
                     /locus_tag="OO357_002999"
                     /pseudo
     CDS             15731..15930
                     /locus_tag="OO357_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase"
BASE COUNT         3953 a         4119 c         3854 g         4004 t
ORIGIN      
        1 gatacgcagc ggaaatgcca gtctttggaa aaatgcaaac tgtttattct cgttaagctg
       61 caacattgtt ttccttgcgg gttgtcccga gcctctttga cggatgtaat taaaatgttg
      121 aatcaaaata catagatatt aattatgatc gtggcaaatt ttattcatta aatcaattaa
      181 atcacttaaa cctgcgagtc atttcgtaat tacattaatt aaaaaactta aaaccattaa
      241 ataaataaaa aaccactaac tctatgtgaa ataaatcaaa atttcacgcc gaaatactcc
      301 ttaggatgta tagcaaaagg agaaaaagat atacctcgat cacccccctt tctcccaagt
      361 gaaaataaaa ggttatcagt ttgcaacatt gaacaacatt cgttgcaaat cgataacaac
      421 atgcaccttc aggatactat ttattatgtt cggcaatgat attttcaccc gcgtaaaacg
      481 ttcagaaaat aaaaaaatgg cggaaatcgc ccaattcctg catgaaaatg atttgagcgt
      541 tgacaccaca gtcgaagtat ttattaccgt aacccgcgat gaaaagctta tcgcgtgcgg
      601 tggaattgcc ggaaatatta ttaaatgcgt tgctatcagt gaatccgttc gcggtgaagg
      661 actggcgctg acattagcca ctgaactgat aaacctcgcc tatgagcggc acagcacgca
      721 tctgtttatt tataccaaaa ccgaatacga ggcgctgttc cgccagtgcg gtttttccac
      781 gctgacctgc gtacccggcg tgatggtgct gatggaaaac agcgccacgc gactgaaacg
      841 ctatgccgaa tcgctgaaaa aatttcgtca tccagggaac aagattggct gcattgtgat
      901 gaacgccaat ccctttacga atggtcaccg ttatctgatt caacaagctg cggcacagtg
      961 cgactggttg catctgtttt tagtcaaaga agattcttca cgcttcccct atgaagaccg
     1021 gctggatctg gtgttaaaag gcaccgccga tattccacgc ctgactgtgc atcgcggctc
     1081 agaatacatc atctcccgcg ctacgttccc ttgctacttc attaaagaac agagcgtcat
     1141 taaccattgt tacaccgaaa ttgatctgaa gattttccgt cagtacctcg ctcccgcgct
     1201 tggtgtaact caccgctttg tcggtactga acccttttgt cgcgttaccg cccagtacaa
     1261 ccaggatatg cgctactggc tggaaacgcc gactatctcc gcagcgccca tcgaactggt
     1321 tgaaattgag cggctgcgtt accaggagat gccgatatcc gcttcccggg tacgtcaact
     1381 gctggcgaaa aacgatctca cggctatcgc gccgctggtc cctgcagtca cgctgcatta
     1441 tttgcagaac ctgcttgagc actcccgcca ggacgcggca gctcgtcaaa agccccccgc
     1501 atgagaaaca ggtgaaaaat gaaaataaac cagcccgccg ttgcaggcac ccttgagtct
     1561 ggggatgtga tgatacgcat cgccccactc gatacgcagg atatcgacct gcaaatcaat
     1621 agcagcgttg agaaacagtt tggcgatgca attcgcacca ccattctgga cgttctcgcc
     1681 cgctacaacg tgcgcggcgt acagctgaat gtcgatgaca aaggcgcact ggactgcatt
     1741 ttacatgcac gactggaagc cctgctggca cgcgccagcg gtatcccggc tctgccatgg
     1801 gaggattgcc aatgatttcc gcttcgctgc aacaacgtaa aactcgcacc cgccgcagca
     1861 tgttgtttgt gcctggtgcc aatgccgcga tggtcagcaa ctccttcatc tacccggctg
     1921 atgccctgat gtttgacctc gaagactccg tggcattacg tgaaaaagac accgcccgcc
     1981 gcatggttta tcacgcgctg caacatccgc tgtatcgcga tattgaaacc attgtgcgtg
     2041 tcaacgcgct ggattccgaa tggggtgtta acgacctgga agccgtcgtt cgcggtggtg
     2101 cggacgttgt gcgtctgccg aaaaccgata ccgctcagga tgttctggat attgaaaaag
     2161 agatcctgcg tatagaaaaa gcctgtggtc gtgaacccgg cagcaccggc ctgctggcgg
     2221 cgattgaatc tccgctgggc attacccgcg cagtggaaat cgctcacgct tccgagcgtt
     2281 tgatcggtat cgccctcggt gcagaagact atgtgcgcaa cctgcgtaca gaacgctccc
     2341 cggaaggaac tgaactgctg ttcgcacgct gttccatttt gcaggccgcg cgctctgcgg
     2401 gtattcaggc gttcgatacc gtctattccg acgctaacaa cgaagccgga ttcctgcaag
     2461 aagccgccca catcaaacag ctgggctttg acggcaaatc gctgatcaac ccgcgtcaga
     2521 ttgatctgct gcacaacctc tacgcaccga cccagaaaga agtggatcac gcccaccgcg
     2581 tcgtagaagc cgctgaagcc gccgctcgcg aaggcctcgg cgtggtttcc ctgaacggca
     2641 agatggtgga cggtccggtt atcgatcgcg cccgtctggt gctctcccgt gcagaacttt
     2701 ccggcatccg cgaagaataa ggcaatcaaa atgacgcaga aaattgaaca atctcaacga
     2761 caagaacggg tagcggcctg gaatcgtcgc actgaatgcg atcttgccgc tttccagaac
     2821 tcaccaaagc aaacctacca ggctgaaaaa gcgcgcgatc gcaaactgtg cgccaacctg
     2881 gaagaagcga ttcgtcgctc aggtttacag gacggcatga cggtttcctt ccatcacgct
     2941 ttccgtggcg gtgacctgac cgtcaatatg gtgatggacg tcatcgcgaa gatgggcttt
     3001 aaaaacctga ccctggcgtc cagctccctg agtgattgcc atgcgccgct ggtagaacac
     3061 attcgccagg gcgtggttac ccgcatttat acctctggcc tacgcggtcc actggcggaa
     3121 gagatctccc gtggtctgct ggcggaaccg gtgcagatcc actctcacgg cggtcgtgtg
     3181 catctggtac agagcggcga actgaatatc gacgtggctt tcctcggcgt cccgtcctgt
     3241 gatgaattcg gtaatgccaa cggctacacc ggtaaagcct gctgcggctc cctcggctat
     3301 gcaatggttg atgccgacaa cgcaaaacag gtcgtgatgc ttaccgaaga actgctgcct
     3361 tatccgcata atccggcaag cattgagcaa gatcaggttg atttgatcgt caaagttaat
     3421 cgcgttggcg atgctgcaaa aatcggcgct ggtactaccc gtatgaccac taacccgcgc
     3481 gaactgctta ttgcccgtag cgctgcggat gtgattgtca actctggcta cttcaaagaa
     3541 ggtttctcca tgcaaaccgg caccggcggc gcttcgctgg cggtaacccg tttcctggaa
     3601 gacaaaatgc gtagccgcga tattcgcgcc gacttcgccc tcggcggtat taccgcgacg
     3661 atggttgatc tgcacgaaaa aggtctgatc cgcaaactgc tggatgtgca gagctttgac
     3721 agccatgctg cgcaatcgct ggcccgtaac cccaatcaca tcgaaatcag cgccaaccag
     3781 tacgctaact ggggttcgaa aggcgcatcg gttgatcgtc tcgacgtggt ggtactgagc
     3841 gcgctggaaa ttgacaggca gttcaacgtt aacgtgctga ccggttctga cggcgtactg
     3901 cgtggcgctt ccggtggtca ctgcgatacc gcgattgcct ctgcgctttc catcatcgtc
     3961 gcgccactgg tacgcggtcg tattccgact ctggtggata acgtgctgac ctgcatcacc
     4021 ccgggctcca gtgtcgatat tctggtcaca gaccacggta tcgcagttaa cccggcacgt
     4081 ccggaactag cagaacgtct gcaggaagcg ggcattaaag tggtttccat tgagtggctg
     4141 cgcgaacgtg cgcgtctgct gaccggtgaa ccacagccga ttgaattcac cgaccgcgtc
     4201 gttgccgttg tgcgttaccg cgatggctcg gtgatcgatg ttgtgcatca ggtgaaggaa
     4261 taagccatgc acctgcttcc tgaactcgcc agccaccatg cggtgtcaat tcccgagctg
     4321 ctcgtcagcc gggatgaaag gcaagcacgg caacacgcct ggctcaagca ccatcctgtt
     4381 ccactggtct cctttaccgt ggttgcgcct gggccgatta aagacagcga ggtcacacgc
     4441 cgaattttta atcatcgcgt gacagccttg cgtgccttag ccacaaagca gggctggcaa
     4501 attcaggagc aggctgcact ggtttccgcc agcgggccgg agggcatgtt gagcattgcc
     4561 gccccggctc gcgacctcaa gctcgccacc attgagcttg aacatagtca tcctctcggg
     4621 cggttatggg atatcgatgt cctgacgccc gaaggcgaaa ttctctcccg ccgcgactat
     4681 tcactgcgcc tcgccgctgc ctgttgtgcg agcaaagcgc agccgtctgc gcgcgtggaa
     4741 aaacccatca actgaccgat ttactcaacc gcatggaggc actgctgaac gatgtcgatg
     4801 cctgcaacgt caactaaaac cacaaagctt gcgacgtcat taatcgatga gtacgccctg
     4861 ctgggctggc gcgccatgct gactgaagtc aatctgtcac cgaaaccagg cctcgtggat
     4921 cgcattaact gcggtgcgca caaagatatg gcgctggaag atttccaccg cagcgcgctg
     4981 gcgattcagg gctggttacc ccgtttcatt gaatttggtg cctgtagtgc agaaatggca
     5041 ccggaagcgg tactccacgg attacgccca attggtatgg cttgcgaagg tgatatgttc
     5101 cgcgccactg cgggcgtaaa cacgcataaa ggcagcattt tttctttagg gctgctatgt
     5161 gcggcaattg gccgtttgct tcagctcaac cagccggtta cgccaacaac cgtttgttct
     5221 acggcggcaa gtttctgccg tggcctgacc gatcgcgaac tgcgtaccaa taattcacga
     5281 ctgacggcag gtcaacggtt gtaccagcag cttggcctta ccggcgcacg cggtgaagcc
     5341 gaagcaggtt atccactggt gatcaaccac gccttgccgc attacctcac tctgctggat
     5401 caggggttag atcctgaact ggcattgctc gataccttgc tcctactgat ggctaccaac
     5461 ggcgatacca acgttgcatc gcgcggtggc gaggggggcc tgcgctggct acagcgcgag
     5521 gcgcaaacat tattgcaaaa agggggcatt cgaaccccca ccgatctcga ttatctccgg
     5581 cagttcgaca gggagtgtat cgaacgaaat ctcagtccag gcggcagtgc cgacctgctg
     5641 atccttacct gttttttagc acagatttaa ttatttaagc acttgataaa tttggaaata
     5701 ttaattttcg gagaacccgt atgtctttag caaaagataa tatatggaaa ctattggccc
     5761 cactggtggt gatgggtgtc atgtttctta tccctgtccc cgacggtatg ccgccgcagg
     5821 catggcatta cttcgctgtg tttgtggcaa tgattgtcgg catgatcctc gagccaattc
     5881 cggcaacagc gatcagtttt attgcggtta ctatttgcgt tattggcagt aattacctgc
     5941 tctttgatgc caaagaatta gctgacccag cgtttaatgc gcaaaaacag gcgctgaaat
     6001 ggggcctggc tggtttttcc agcaccacgg tatggctggt atttggcgca tttatttttg
     6061 cattagggta tgaagtttcc gggttaggtc gtcgcattgc ccttttcctg gtgaaattca
     6121 tggacaaacg cacgctgacg ttgggttatg cgattgtcat tatcgacatt ctgctggcac
     6181 cgtttacacc gtccaacacc gcgcgtaccg ggggtacggt ttttccggtc attaaaaacc
     6241 tgccgccgtt gtttaaatca ttcccgaacg atccgtccgc gcgtcgtatt ggcggctatt
     6301 tgatgtggat gatggtcatt agtaccagtc tgagttcgtc catgtttgtc accggtgcgg
     6361 accaaacgtg ctgggtctgg agttcgtcag caaaattgcc ggtatccaga ttagctggtt
     6421 gcagtggttc ctctgcttcc tgccggttgg ggttatcttg cttatcattg cgccgtggct
     6481 ttcctacgtg ctgtacaaac cggaaatcac acacagtgaa gaagtggcaa cctgggcggg
     6541 tgatgaacta aaaaccatgg gtgcgctgac acgcagagag tggacgctga ttggccttgt
     6601 attgctcagc ttaggtttgt gggtatttgg cagtgaagtc attaatgcta ctgcggttgg
     6661 tctgctggca gtttcgctaa tgctggctct gcacgttgtg ccgtggaaag acattacccg
     6721 ctataacagc gcatggaaca cgctggtcaa cctggcaact ctggttgtga tggctaacgg
     6781 cctgactcgt tctggtttta ttgactggtt cgccggtacc atgagtacgc acctggaagg
     6841 attctcacca aacgcaacgg tgattgtact ggttctggtg ttctactttg cacactacct
     6901 gtttgccagc ctgtctgcgc acaccgcaac catgctgccg gttattctgg ccgtcggtaa
     6961 aggtattccg ggcgtaccaa tggaacaact gtgtatcctg ctggtgctgt ctatcggtat
     7021 catgggctgt ctgacgccgt atgcaaccgg tcctggggtg attatttacg gctgtggcta
     7081 tgtgaaatca aaagattact ggcgtcttgg cgcaatcttc ggggtgattt acatctctat
     7141 gttgctgttg gttggctggc cgattctcgc catgtggaac taatacgttc gccaaacgct
     7201 gaataaatca tcacgcccgc caggtaagcc cacctggcgg gcttttttat gatttaatag
     7261 atagtattca gtgtatgtat gagttccaca cccattatga aagcattctg ccgtaacgcc
     7321 gcgttgctcg cggtttctct gcttccctta tcttctgcca acgccgtagc gttgcaggca
     7381 aaacagtatg gcgattttga tcgctatgtc ctggccctct cctggcaaac cggattttgc
     7441 cagagtcaac acgatcgaaa tcgtaacgaa cgagatgaat gtcgcctgca aaccgaaacg
     7501 accaacaaaa ctgattttct gaccgtacat ggtctgtggc caggattgcc taaatcggtt
     7561 gctgcccgtg gtgttgatga acgccgctgg atgcgcttcg gttgcgctac tcgcccaatc
     7621 ccgaatctac cagaagcacg cgccagccga atgtgttcat cgccggaaac cgggttatca
     7681 ctggaaacgg ccgctaaact aagtgaagtc atgccaggag ctggcggacg ttcctgcctg
     7741 gaacgctacg aatatgccaa acacggtgcc tgctttggtt ttgatccgga cgcatacttc
     7801 ggtacgatgg tacgcctgaa tcaagaaatt aaagagagcg aagccggaaa attccttgcg
     7861 gataattacg gtaaaacagt gagccgccgt gactttgacg ccgcctttgc caaaagctgg
     7921 ggaaaagaga acgtgaaagc agttaagcta acgtgccagg gtaaccctgc gtatttgact
     7981 gaaattcaga tctcgatcaa agctgacgcc atcaacgctc cgctttctgc aaactcattc
     8041 ttgccacaac ctcacccagg taactgtggc aaaacctttg tgattgataa agcgggttat
     8101 taatcctctg atttacaaaa cacgctaatt cgtcttccat aaccatgccg attaagaatt
     8161 atacataatg cgctgcattg aggaattccc tgaatgtgac gcaaatcact tcaaagagtg
     8221 agcaacagtc agtatcatca tgtgagttaa accctcgccg cctgacggtg agggttttct
     8281 tttgggtatg tttatctgcg acactcgcag taccgacgac atggagtaaa aatgtccaga
     8341 ccaactatca tcattaacga cctggatgcc gaacgcatcg atattctgct ggagcaaccc
     8401 gcctatgctg gtttgccaat cgccgacgcg ttaaacgcag agttggatcg cgcccaaatg
     8461 tgttcgccag aagagatgcc acacgacgtg gtgacaatga acagccgggt taaattccgc
     8521 aatcttagcg atggcgaagt gcgtgtgcgc acgctggtgt atccggcaaa aatgaccgat
     8581 agcaatactc agctttccgt tatggctccg gtaggtgccg cactgctggg gctgcgcgtt
     8641 ggcgattcca ttcactggga acttccgggc ggcgttgcaa cccaccttga agtgctggaa
     8701 ctcgaatacc agccagaagc tgctggcgac tacctgcttt aatcccatct gcacaagcga
     8761 taactcgctt tgcccagagg atgcactcgc atcctcttta cgcaaaatca gcaatttttc
     8821 ctggttctca ctgttcactt ttgctgcaaa attattccgt ttcgtttgtg ggaataacca
     8881 tggcagaagc attttacatc ctgataggat ttctgattat ggcggcgatt atcgtcatgg
     8941 ctgtccttta cctcgaaaac cactcctgac gatcatattg ttccccccgg catcgcattc
     9001 accagacctg aaaccgcctt ctgcgccgcc tctgcgcttt gtgcaccggg taccagaatc
     9061 accttacggc actcctcttc acgtttttcg aaaatctcat atccccgggc ggcctcttca
     9121 aacggcatat agtgggtaac aatttcttct ggtttcagta atcctttctc aattaacggt
     9181 aataattctc ccagccatgc gtgaacgtgg gtctgtccca ttttaaacgt caacccttta
     9241 tcaaaggcgt cgccaaacag gaaaccgtga ataaatccag cgtagacgcc cggtacgcta
     9301 acaatgccgc cacgcctgac cgccgcaata cactggcgca acgctttacc gctgctgccc
     9361 tccagtttca ggttagtcag caccgtttcc gtggtgctgc ctttcgcttc aaaaccgacg
     9421 gcgtctatta ctgcatccac gccccggtga cctgccgttt gttcaataat tgactgtgcc
     9481 ggatcgctat cttcatcgaa attaatcggg atcgcgccgt agcggtcagc ggcgaaacgc
     9541 aagcggtagg gatgatgatc aacaacaaaa atctgttccg caccgagcaa ccgtgcacag
     9601 gcgattgtca acaatcccac aggaccagca ccatagactg caacgcttga accttgttgg
     9661 atctgcacat tttttgctgc ctgccatgcc gttggcagaa tatcagaaag gaaaagggct
     9721 ttatcatctg aaagcaaagg cggtacttta aacggcccca cattcccttt cgggacgcgg
     9781 acatattccg cctgcccgcc aggaacgcca ccatacaggt gactataacc aaacaatgcc
     9841 gcgggagcag gtatctgttt tttattgagc gcagcgcctt tacccgcatt ggtattttcg
     9901 caggcggcat attgctgtaa tcgacagaaa aaacagtcgc cacaagcaat gacgaacgga
     9961 attaccaccc ggtcgccttt ttgcaaattt tttacgtcct tcccggtttc aacgacttcc
    10021 cccataaatt catgaccaaa aatatcgcca tgtttaacct ggggtatttt gcctcgataa
    10081 agatggaggt cagagccaca gatcgccgtt accgtaatac gcagaataat atcatctgcc
    10141 tgttcaatgc ccggatcggg aacattttct acctgaacgt gatgtgggcc gtgataagtc
    10201 aatgctttca tgcaacctcc agagacagtt caaaaaagag tcagtcttta agggtagcta
    10261 tcacagcggg aaaacgggcg gtaccgcagg ttttgggaga attcctagtg ttcagtgaga
    10321 atatattaat cacagaaacc gttcgtaagg aaaaagaacc agacaaatat cagggataaa
    10381 tattataatc atggagaatg atttgttcat gattaacagg gagaaagggt tatgtataag
    10441 gcaatcatta tgccagttga tgtatttgaa atggaattga gcgacaaagc tgttcgccac
    10501 gctgaattcc tcgcccagga tgacggagtt attcatctac ttcacgtact acccgggtca
    10561 gccagcctga gcctgcaccg ttttgccgct gatgtgcgtc gttttgaaga gcatctgcaa
    10621 catgaagcag aagaacgtct gcaaacgatg gtcagccact tcaccatcga tccttcccgc
    10681 attaaacaac atgtccgttt tggtagcgtg cgggatgaag tcaatgagtt ggcagaagaa
    10741 ctgggggctg atgttgtagt tatcggttct cgcaacccat cgatttcgac ccatctgtta
    10801 ggttctaacg cctcgagcgt gatccgccac gccaatctgc ctgtactggt cgttcgctaa
    10861 cagataaaag tatttatgcc gccctgcctg gaacatcccg gcagggcttt ttaatgccat
    10921 aaaaaagccg ccaggtttga ccccggcggc taagcaattg caggtgaatc ttacttcttc
    10981 ttatgcagtt ttggtgcgaa tcaggtagtc aaaagcactc agagaggctt tggcaccttc
    11041 gccagtggcg atgatgatct gcttgtacgg aaccgtcgta cagtcacccg ctgcgaacac
    11101 gcctttgacg ttggtttcac atttcgcatc aatgataatc tcgcccatgc ggttacgttc
    11161 gactgcgcct tccagccagt tggtgttcgg cagcagacca atctggacaa aaataccggc
    11221 cagttcgatg ttgtgaatat tgccgctgac acgatctcga tattccagac caacgacttt
    11281 gctgccgtcg cctttcactt ccgtggtttg cgcattcaga ataatgtcga cgtttttcag
    11341 gctgcgcagt ttgtcctgca gaacctggtc ggctttcatt tctggcgcaa attccagcag
    11401 cgttacgtgc tcaacgatac ccgccaggtc gattgccgct tccacgccgg agttaccgcc
    11461 gccgataacc gctacgcgtt tgcctttaaa cagcgggccg tcgcagtgcg ggcagtaggt
    11521 cacgccttta gtgcgatact gatcttcgcc cggaacgttc atgttgcgcc attttgcacc
    11581 cgtcgccacg ataatgctgc gtgctttcag taccgcgcca gaagctgttt caatctggtg
    11641 tagaccacct tcaaccgctg ccgggatcag tttgctggcg ctctggctgt cgatcacatc
    11701 aacgtcgtat tcatcaacgt gaactttcag tgcgcctgcc aacttctgcc cttcagtctt
    11761 cggtacagaa atgtagtttt cgatatcaac ggtatcgagg atctgaccac caaaacgttc
    11821 gcccatcaga ccggtacgga tgcctttacg tgcggagtaa attgctgccg ctgcacccgc
    11881 cgggccggaa ccaacgatta atacgtcata agcatcacgc ttgttcagct cttctgccgc
    11941 acgattttcc gcgccagtat caattttggc aacgatttca gtcaacgtca tgcggccctg
    12001 accaaactct ttcccgttta cgaacactgc cggaacgccc atcacgttgc gatcggtgat
    12061 ttcgttctgg aaggtgccgc cgtcaattgc agtgtgcttg atgcgcgggt tcaatacgct
    12121 catcaggttc agcgcctgca ccacgtccgg gcagttatgg caagagagcg agtaataggt
    12181 ttcgaattca aaatcaccgt caatatggcg aatctgctcc agcagagact gcgcttcttt
    12241 cgacggatga ccaccggtcc acagcaacgc cagtaccagc gaggtgaact cgtggcccag
    12301 cggggagcct gcaaaacgtg gtccctggtt ggaacctggg ttggtgatca ggaaagacgg
    12361 cttacgcact ggcaagctgt tatcttcttt aaaggtgact ttgtctgaca gttctgcgat
    12421 ttcagccaac agttccttga tttctgccga tttagcgctg tcatccagcg tggcaattaa
    12481 ctcaacaggc ttggtcaatt tctcaaggta agccttgagt tgagttttca tatttgtgtc
    12541 gagcatgttt atctcctggg ctttaaacat catcatgcaa gcggcctctg cggctacctg
    12601 gatgcagctt gcatcatggt gcggggaaga aaatcgggtg ccgcagcacc cgaagaatta
    12661 ggtgaatttc cgaagtattt cgcgtcagag caaggcgact agcctgtgaa tccccgggag
    12721 cttacacaag taagtgaccg gggtgagcgg gcgacgccaa cgccgctatg gcgtgaaaga
    12781 cgaaggaaat ttagatttta ccaaccaggt ccagagacgg agccagagtt gcttcacctt
    12841 ctttccattt agccgggcaa acttcacctg ggtgagaagc tacgtactga gctgctttga
    12901 ttttacgcag cagatcagac gcgtcacggc caatgccttc agcggtaact tcgattgcct
    12961 ggatgatacc ctgcgggtca acaacgaagg tcgcacggtc agccagacct tcatcttcac
    13021 gcatgttgtc gaagttacgg gtcagggcgc cagtcgggtc gccgatcatc gcatatttga
    13081 ttttagcgat ggtttcagag ctgctgtgcc atgctttgtg ggtgaagtga gtatcggtag
    13141 atactgcgta tacgtctacg cccagtttct gcagttcttc gtagtggtca gcaacgtcac
    13201 ccagttcggt cgggcatacg aaagtaaagt cagccgggta gaagaagaag acgctccagc
    13261 ggccttcggt atctttttcg gtgatttcga tgaattcgcc gtttttgaat gcctggtttt
    13321 taaaaggttt aattttggtg ttaatcaagg acatctatac ttcctccgtg ttttcgatga
    13381 gatgtaaggt aaccaatttt tgccgattcg gctaatgcgt ttccattatc aaatcgataa
    13441 gttttacctt acaaccttcg taagacaact ttgtgaactt tgcaccgcca gcggcgataa
    13501 aaagtaaaat ggcccccttt ccaggtgacc gtgtaatctg aactaccctt tggtatcttc
    13561 agcgcctgcc tgcaggctga ggtgttgccc ttacaaatgc aacaacgaca tggattacaa
    13621 caccctcata aacaaagggc aatcacctga tctaagctct tacctatgac agtgataggt
    13681 tatgcctttt actcgacttt tgcactgact gaaaagaaca aattaatgtt aaaaaagata
    13741 cttttactgg ctctgcttcc tgcaatcgcc ttcgcagagg aacttccttc cccagtaaaa
    13801 gcgattgaaa aacagggcat tacaatcatc aaaacgttcg atgcccccgg aggaatgaaa
    13861 ggttatctcg gaaagtatca ggatatgggc gtcaccatct acctgactcc cgatggtaag
    13921 cacgctatct ctggttacat gtacaacgag aaaggtgaaa acctgagtaa cacacttatc
    13981 gaaaaagaaa tttacgcacc agccggacgc gaaatgtggc aacggatgga acaatcccac
    14041 tggctcctcg acggtaaaaa agatgcgccg gtcattgtct acgtcttctc cgatccgttc
    14101 tgcccatatt gtaaacagtt ctggcagcag gcgcgcccgt gggtagattc tggcaaagtg
    14161 caattaagaa cattgttggt tggggttatc aagccagaaa gcccggcgac agcagcggca
    14221 attcttgcct ccaaagatcc cgcaaaaacc tggcaacaat atgaagcctc tggttgcaag
    14281 cttaagctaa acgtgcctgc aaacgtaagt acagagcaaa tgaaagtgtt aagtgacaat
    14341 gagaaactga tggacgatct gggggcaaat gtcacgccgg ctatctatta catgagtaag
    14401 gaaaatacgc tgcaacaggc cgtggggttg cccgatcaga aaacgcttaa tatcattatg
    14461 ggaaataaat aaaaggtaat tcatggatgg ttgaactata tccctgcttt tcaaccatcc
    14521 taaaaaagca tgaatttctc gctctaccct aagtggctgt gctattttag aaaaacttaa
    14581 tcctgagtta agcaaaaaaa cataatcaat aaaaatacat acgcaaaaaa cattgattaa
    14641 gtgaatatac catggaagaa aaatataaac cggagtagtg tatggccaat ctctacgact
    14701 tgaaaaagtt cgacttaaat cttcttgtca tatttgagtg tatttaccag catttaagca
    14761 ttagcaaagc tgctgaatca ctatatatca ccccatcagc cgtgagccag tcgttacaac
    14821 gcttacgtgc gcaatttaat gaccctttat tcattcgctc aggaaaaggt atcgcgccga
    14881 cgacaacagg gttgaatctg catcaccatc ttgagaaaaa cctcaaaggc cttgaacaaa
    14941 caattaatat agtcaataag tccgaactaa agaaaaactt tattatctac ggccctcagc
    15001 tcatttcttg ctctaataat agtatgctga tccgctgcct acggcaagat gcctctgtcg
    15061 agattgagtg ccatgatatc ctcatgtcag cagagaatgc tgaagaactg ttggttcacc
    15121 gcaaagccga tctggtgatc acccaaatgc cagtgatcag ccgttccgtc atttgcatgc
    15181 cattgcatac aattcgcaat actctaattt gtagtaataa gcatccgcga attactgaca
    15241 atagcactta tgaacaaatt atggcagaag agttcaccca attaatagcc aaatcagcag
    15301 gagttgatga tattcaaatg gaaatagacg agaagtttat gaatcgcaaa atttcatttc
    15361 gtggttcctc tttgctgaca ataattaata gcattgcagt tacagattta ctgggaatag
    15421 tcccctatga actttataac taccaccgtg actttcttaa tttaaaagag ataaaactag
    15481 aacatccgtt accttcaata aagctttaca tttcctataa taaatcttct ttaaataatt
    15541 tggtgttctc ccgatttatt gaccgtctaa atgagagttt ttgatatata actatagaca
    15601 gctactataa ctttcatcta tttattcaca gcgcgaatga aaaataaaac actctgcttt
    15661 acagtgttta ctttataatt aacagtctta tctcactgtt caccctgtaa cgttcagaga
    15721 gcgtcttcag atgtctattt ataaaattcc tcttccgctc aatattctgg aagctgcacg
    15781 agagcgtatt acctagacac tcaacaccct accccgcgtt tgtgtctcat tttctggcgg
    15841 caaagactct ggtttaatgc tgcatctgac agccgaactt gcccgacaaa tgggcaaaaa
    15901 aatctgcgtt ttgtttatca actgggaggc
//
LOCUS       DAJTRC010000085        15517 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.085, whole genome shotgun sequence.
ACCESSION   DAJTRC010000085 DAJTRC010000000
VERSION     DAJTRC010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15517)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15517)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15517
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.085"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(71..1282)
                     /locus_tag="OO357_003000"
     CDS             complement(71..1282)
                     /locus_tag="OO357_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001218317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase arm-type DNA-binding domain-containing
                     protein"
                     /protein_id="HCR5887866.1"
                     /translation="MALSDVKVRSAKPEAKAYKLTDGEGMVLLVHPNGSKYWRLRYRF
                     GGKEKMLALGKYPEVSLADARARRDEARKLLANGVDPSENKKAVKVEQEQEAITFEVV
                     ARDWHASNQKWSASHSARVLKSLEDNLFTAIGKRNIAELKTRDLLVPIKAVESSGRLE
                     VAARLQQRTTAIMRFAVQSGLIDYNPAQEIAGAVATAKRQHRAALELNRIPELLHRID
                     HYSGRPLTRLAVELTLLVFIRSSELRFARWSEIDFETAMWTIPGEREQLEGVKHSQRG
                     SKMRTPHLVPLSRQALSILEKIKSMSGNRELIFVGDHDPRKPMSENTVNKALRVMGYD
                     TKVEVCGHGFRTMACSSLIESGLWSRDAVERQMSHQERSSVRAAYIHKAEHLGERRLM
                     FEVVNKNWPPS"
     gene            complement(1481..1565)
                     /locus_tag="OO357_003001"
     tRNA            complement(1481..1565)
                     /locus_tag="OO357_003001"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1529..1531),aa:Leu,seq:caa)
     gene            1761..2780
                     /gene="ahr"
                     /locus_tag="OO357_003002"
     CDS             1761..2780
                     /gene="ahr"
                     /locus_tag="OO357_003002"
                     /EC_number="1.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent aldehyde reductase Ahr"
                     /protein_id="HCR5887867.1"
                     /translation="MSMIKSYAAKEAGGELEVYEYDPGELKPQDVEVQVDYCGICHSD
                     LSMIDNEWGFSQYPLVAGHEVIGRVVALGSAAQDKGLQVGQRVGIGWTARSCGHCDAC
                     ISGNQINCEQGAVPTIMNRGGFAEKLRADWQWVIPLPENIDIESAGPLLCGGITVFKP
                     LLMHHITATSRVGVIGIGGLGHIAIKLLHAMGCEVTAFSSNPAKEQEVLAMGADKVVN
                     SRDPQALKALSGQFDLIINTVNVSLDWQPYFEALTYGGNFHTVGAVLTPLSVPAFTLI
                     AGDRSISGSATGTPYELRKLMRFAARSKVAPTTELFPMSKINDAIQHVRDGKARYRVV
                     LKADF"
     gene            2909..4411
                     /locus_tag="OO357_003003"
     CDS             2909..4411
                     /locus_tag="OO357_003003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF853 domain-containing protein"
                     /protein_id="HCR5887868.1"
                     /translation="MSEPLLIARTPDTELFLLPGMANRHGLITGATGTGKTVTLQKLA
                     ESLSEIGVPVFMADVKGDLTGIAQAGTASEKLLTRLKNIGVNDWQPHANPVVVWDIFG
                     EKGHTVRATVSDLGPLLLARLLNLNDVQSGVLNIIFRIADDQGLLLLDFKDLRAITQY
                     IGDNAKSFQNQYGNISSASVGAIQRGLLSLEQQGAAHFFGEPMLDIKDWMRTDANGKG
                     VINILSAEKLYQMPKLYAASLLWMLSELYEQLPEAGDLEKPKLVFFFDEAHLLFNDAP
                     QVLLDKIEQVIRLIRSKGVGVWFVSQNPSDIPDNVFGQLGNRVQHALRAFTPKDQKAV
                     KTAAQTMRANPAFDTEKAIQELGTGEALISFLDAKGSPSVVERAMVIAPCSRMGPVTE
                     DERNGLINHSPVYGKYEDEVDRESAYEMLQKGFQASTEQQNNPPVKGKEVAVDDGILG
                     GLKDILFGTTGPRGGKKDGVVQTMAKSAARQVTNQIVRGMLGSLLGGRKR"
     gene            complement(4530..5612)
                     /gene="lptG"
                     /locus_tag="OO357_003004"
     CDS             complement(4530..5612)
                     /gene="lptG"
                     /locus_tag="OO357_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709939.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HCR5887869.1"
                     /translation="MQPFGVLDRYIGKTIFTTIMMTLFMLVSLSGIIKFVDQLKKAGQ
                     GSYDALGAGMYTLLSVPKDVQIFFPMAALLGALLGLGMLAQRSELVVMQASGFTRMQV
                     ALSVMKTAIPLVLLTMAIGEWVAPQGEQMARNYRAQAMYGGSLLSTQQGLWAKDGNNF
                     VYIERVKGDEVLGGISIYAFNENRRLQSVRYAATAKFDPEHKVWRLSQVDESDLTNPK
                     QITGSQTVSGTWKTDLTPDKLGVVALDPDALSISGLHNYVKYLKSSGQDAGRYQLNMW
                     SKIFQPLSVAVMMLMALSFIFGPLRSVPMGVRVVTGISFGFVFYVLDQIFGPLTLVYG
                     IPPIIGALLPSASFFLISLWLLMRKS"
     gene            complement(5612..6712)
                     /gene="lptF"
                     /locus_tag="OO357_003005"
     CDS             complement(5612..6712)
                     /gene="lptF"
                     /locus_tag="OO357_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HCR5887870.1"
                     /translation="MIIIRYLVRETLKSQLAILFILLLIFFCQKLVRILGAAVDGDIP
                     ANLVLSLLGLGVPEMAQLILPLSLFLGLLMTLGKLYTESEITVMHACGLSKAVLVKAA
                     MILAVFTAIVAAVNVMWAGPWSSRHQDEVLAEAKANPGMAALAQGQFQQATNGSSVLF
                     IESVDGSDFKDVFLAQIRPKGNARPSVVVADSGHLTQLRDGSQVVTLNQGTRFEGTAL
                     LRDFRITDFQDYQAIIGHQAVALDPNDTDQMDMRTLWNTDTDRARAELNWRITLVFTV
                     FMMALMVVPLSVVNPRQGRVLSMLPAMLLYLLFFLIQTSLKSNGGKGKLDPTLWMWTV
                     NLIYLALAIVLNLWDTVPVRRLRASFSRKGAV"
     gene            6979..8490
                     /gene="pepA"
                     /locus_tag="OO357_003006"
     CDS             6979..8490
                     /gene="pepA"
                     /locus_tag="OO357_003006"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HCR5887871.1"
                     /translation="MEFSVKSGSPEKQRSACIVVGVFEPRRLSPIAEQLDKISDGYIS
                     ALLRRGELEGKPGQTLLLHHVPNVLSERILLIGCGKERELDERQYKQVIQKTINTLND
                     TGSMEAVCFLTELHVKGRNNYWKVRQAVETAKETLYSFDQLKTNKSEPRRPLRKMVFN
                     VPTRRELTSGERAIQHGQAIAAGIKAAKDLGNMPPNICNAAYLASQARQLADSYSKNV
                     ITRVIGEQQMKELGMHSYLAVGQGSQNESLMSVIEYKGNASEDARPIVLVGKGLTFDS
                     GGISIKPSEGMDEMKYDMCGAAAVYGVMRMVAELQLPINVIGVLAGCENMPGGRAYRP
                     GDVLTTMSGQTVEVLNTDAEGRLVLCDVLTYVERFEPEAVIDVATLTGACVIALGHHI
                     TGLMTNHNPLAHELIAASEQSGDRAWRLPLGDEYQEQLESNFADMANIGGRPGGAITA
                     GCFLSRFTRKYNWAHLDIAGTAWRSGKAKGATGRPVALLAQFLLNRAGFNGEE"
     gene            8624..9067
                     /gene="holC"
                     /locus_tag="OO357_003007"
     CDS             8624..9067
                     /gene="holC"
                     /locus_tag="OO357_003007"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004098129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HCR5887872.1"
                     /translation="MKNATFYLLDNDTTVDGLSAVEQLVCEIAAERWRSGKRVLIACE
                     DEKQAYRLDEALWARPAESFVPHNLAGEGPRGSAPVEIAWPQKRSSSPRDILISLRTS
                     FADFATAFTEVVDFVPYEDSLKQLARERYKAYRVAGFNLNTATWK"
     gene            9067..11922
                     /gene="valS"
                     /locus_tag="OO357_003008"
     CDS             9067..11922
                     /gene="valS"
                     /locus_tag="OO357_003008"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000416368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HCR5887873.1"
                     /translation="MEKTYNPQDIEQPLYEHWEKQGYFKPNGDESQESFCIMIPPPNV
                     TGSLHMGHAFQQTIMDTMIRYQRMQGKNTLWQVGTDHAGIATQMVVERKIAAEEGKTR
                     HDYGREAFIDKIWEWKAESGGTITRQMRRLGNSVDWERERFTMDEGLSNAVKEVFVRL
                     YKEDLIYRGKRLVNWDPKLRTAISDLEVENRESKGSMWHIRYPLADGAKTADGKDYLV
                     VATTRPETLLGDTGVAVNPEDPRYKDLIGKYVILPLVNRRIPIVGDEHADMEKGTGCV
                     KITPAHDFNDYEVGKRHALPMINILTFDGDIRESAQVFDTKGNESDVYSSEIPAEFQK
                     LERFAARKAVVAAVDALGLLEEIKPHDLTVPYGDRGGVVIEPMLTDQWYVRADVLAKP
                     AVEAVENGDIQFVPKQYENMYFSWMRDIQDWCISRQLWWGHRIPAWYDEAGNVYVGRN
                     EEEVRKENNLGANVALRQDEDVLDTWFSSALWTFSTLGWPENTDALRQFHPTSVMVSG
                     FDIIFFWIARMIMMTMHFIKDENGKPQVPFHTVYMTGLIRDDEGQKMSKSKGNVIDPL
                     DMVDGISLPELLEKRTGNMMQPQLADKIRKRTEKQFPNGIEPHGTDALRFTLAALAST
                     GRDINWDMKRLEGYRNFCNKLWNASRFVLMNTEGQDCGFNGGEMTLSLADRWILAEFN
                     QTIKAYREALDSFRFDIAAGILYEFTWNQFCDWYLELTKPVMNGGTEAELRGTRHTLV
                     TVLEGLLRLAHPIIPFITETIWQRVKVLCGITADTIMLQPFPQYDASQVDEAALADTE
                     WLKQAIVAVRNIRAEMNIAPGKPLELLLRGCSADAERRVNENRGFLQTLARLESITVL
                     PADDKGPVSVTKIIDGAELLIPMAGLINKEDELARLAKEVAKIEGEISRIENKLANEG
                     FVARAPEAVIAKEREKLEGYAEAKAKLIEQQAVIAAL"
     gene            complement(11976..13172)
                     /locus_tag="OO357_003009"
     CDS             complement(11976..13172)
                     /locus_tag="OO357_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709944.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF898 domain-containing protein"
                     /protein_id="HCR5887874.1"
                     /translation="MAQVINEMDVPSHSFVFHGTGERYFLICVVNVLLTIITLGIYLP
                     WALMKCKRYLYANMEVNGQRFSYGITGGNVFVSCLVFVFFYFAILMTVSADMPLVGCV
                     LTLLLLVLLIFMAAKGLRYQALMTSLNGVRFSFNCSLKGFWWVTFFLPILMAIGMGTV
                     FFISTKMLHANSSSSVIISVVLMAIVGIVSIGIFNGTLYSLVMSFLWSNTSFGIHRFK
                     VKLDTAYCIKYAILAFLALLPFLAVAGYIIFDQILNEYDSSGYANDDIENLQQFMEMQ
                     RKMIIAQLIYYFGIAVSTSYLTVSLRNHFMSNLSLNDGRIRFRSTLTYHGMLYRMCAL
                     VVISGITGGLAYPLLKIWMIDWQAKNTYLLGDLDDLPLINKEEQPDKGFLASISRGVM
                     PSLPFL"
     gene            13365..13868
                     /locus_tag="OO357_003010"
     CDS             13365..13868
                     /locus_tag="OO357_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5887875.1"
                     /translation="MNNIAPQSPVMRRLTLQDNPAIASVIRQVYAEYGLTADKGYTVA
                     DPNLDELYQVYSQPGHAYWVVEYNGEVVGGGGIAPLTGSEADICELQKMYFLPAIRGK
                     GLAKKLALMAMEQAREMGFKRCYLETTAFLKEAIALYEHLGFEHIDYALGCTGHVDCE
                     VRMLREL"
     gene            complement(13914..14330)
                     /gene="rraB"
                     /locus_tag="OO357_003011"
     CDS             complement(13914..14330)
                     /gene="rraB"
                     /locus_tag="OO357_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E inhibitor RraB"
                     /protein_id="HCR5887876.1"
                     /translation="MANPEQLEEQREETRLIIEELLEDGSDPDALYTIEHHLSADDLE
                     TLEKAAVEAFKLGYEVTDPEELEVEDGDIVICCDILSECALNADLIDAQVEQLMTLAE
                     KFDVEYDGWGTYFEDPNGEDGDDEDFVDEDDDGVRH"
     gene            14492..15505
                     /gene="argF"
                     /locus_tag="OO357_003012"
     CDS             14492..15505
                     /gene="argF"
                     /locus_tag="OO357_003012"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HCR5887877.1"
                     /translation="MSGFYHKHFLKLLDFTPAELNSLLQLAAKLKADKKSGKEEAKLT
                     GKNIALIFEKDSTRTRCSFEVAAYDQGARVTYLGPSGSQIGHKESIKDTARVLGRMYD
                     GIQYRGYGQEIVETLAEYAGVPVWNGLTNEFHPTQLLADLLTMQEHLPGKAFNEMTLV
                     YAGDARNNMGNSMLEAAALTGLDLRLVAPQACWPEAALVTECRALAQQNGGNITLTED
                     VAKGVEGADFIYTDVWVSMGEAKEKWAERIALLRDYQVNSKMMQLTGNPEVKFLHCLP
                     AFHDDQTTLGKKMAEEFGLHGGMEVTDEVFESAASIVFDQAENRMHTIKAVMVATLSK
                     LNN"
BASE COUNT         4012 a         3959 c         3990 g         3556 t
ORIGIN      
        1 gcactaagac cgcacgaata taacggtggg ttacccccaa acgaatcgga aaatcgatac
       61 tggaaaaact ctaactcggt ggccagtttt tgttgaccac ttcaaacatc aacctacgct
      121 ccccaagatg ttccgcctta tggatgtaag ctgctcgcac agagctacgc tcctggtgac
      181 tcatctgccg ctctaccgca tccctcgacc ataatcccga ctcaatcaac gaactacaag
      241 ccatcgtcct gaagccatgc ccacaaacct cgaccttcgt gtcatacccc attacgcgca
      301 aagccttgtt caccgtattc tcactcatcg gcttacgtgg atcgtgatcg cccacaaaaa
      361 tcagctctcg atttccgctc atgcttttga tcttttccaa aatacttagg gcctgccgcg
      421 acaaaggtac aaggtgtggt gtccgcatct tcgagccacg ctgagaatgc ttgactcctt
      481 ccagttgctc acgctcacct gggattgtcc acatagccgt ttcaaaatct atttctgacc
      541 agcgggcaaa acgcagctcg ctcgaacgga tgaaaaccaa taaagtgagt tcaaccgcca
      601 gtcgagttaa cggtctgcca gaatagtgat ctatgcgatg aagtaattca ggtatacggt
      661 tcagttcaag agctgcacgg tgctgtcttt tcgccgtagc aaccgcacct gcaatctctt
      721 gcgcggggtt gtagtcgatt aagccgctct gaacggcaaa gcgcataatc gcggtagtac
      781 gctgctgtaa acgggcggca acttcgagcc gcccggatga ctcgactgct ttaatgggta
      841 caagaagatc ccgcgtcttc agttccgcaa tgttccgctt accgatagca gtaaagagat
      901 tatcctccag acttttcaaa acacgagcgc tatgcgatgc cgaccacttc tgattgctgg
      961 cgtgccagtc tctggcgact acttcaaacg ttatcgcctc ttgttcctgt tctaccttaa
     1021 cagctttctt gttttcactg ggatcgacac cattagctaa aagcttacga gcctcatccc
     1081 ggcgtgccct ggcatccgcc aacgacactt cggggtattt ccccaatgcc agcatcttct
     1141 ctttgccgcc aaagcgataa cgtagccgcc agtatttgga gccgttaggg tgaaccagca
     1201 aaaccatgcc ttcaccgtcg gtcagcttat aggcttttgc ttcaggctta gccgaacgaa
     1261 ccttcacatc actcagagcc atgatgagta tcctttcaag ggttctgtgt gggtacaaac
     1321 attatcgaac cgggaaatac ccgcagttgt acccgcatca gtaagttgat gtagattgaa
     1381 tcaggttgac ttaggttgag tgaaaaagcg aggaaagcct tgcggatact ggattttagg
     1441 cacaaaaaaa gacgtccgtt gacgtctatt gatgttccga tggtgccgaa ggccggactc
     1501 gaaccggcac gtatttctac ggttgatttt gaatcaactg cgtctaccga tttcgccact
     1561 tcggcactga agaggtatgc ggaaaacgtt gtggattata cctgtcgcac gccaccatgc
     1621 aagcgccagc ttgcacccac ctcgctaagt gctgaaattt tcagcattac actcagcgat
     1681 atgtaacctt tgtcacactc caggcacccc gccctgccat gctctacact tcccaaacaa
     1741 caccagagaa ggccccaaaa atgtcgatga taaaaagcta tgccgcaaaa gaagcgggcg
     1801 gcgaactgga agtttatgag tacgatcccg gtgagctgaa gccacaagat gttgaagtgc
     1861 aggtggatta ctgcgggatt tgtcattccg atctgtcgat gatcgataac gaatggggat
     1921 tttcacaata tccgctggtt gccgggcatg aggtgattgg gcgcgtagtg gcactcggga
     1981 gcgccgcgca ggataaaggt ttgcaggtcg gtcagcgtgt cgggattggc tggacggcgc
     2041 gtagctgtgg tcactgcgac gcctgtatta gcggtaatca gatcaactgc gagcaaggtg
     2101 cggtgccgac aattatgaat cgcggtggct ttgccgagaa gttgcgtgcg gactggcaat
     2161 gggtgattcc actgccagaa aatattgata tcgagtccgc cgggccgctg ttgtgcggcg
     2221 gtatcacggt ctttaaacca ctgttgatgc accatatcac tgctaccagc cgcgttgggg
     2281 taattggtat tggcgggctg gggcatatcg ctataaaact tctgcacgca atgggatgcg
     2341 aggtgacggc ctttagttct aatccggcga aagagcagga agtactggcg atgggtgccg
     2401 ataaagtggt gaatagccgc gatccgcagg cactgaaagc cctgtcgggg cagtttgatc
     2461 tcattatcaa tactgtgaac gtcagcctcg actggcagcc ttattttgag gcgctgacct
     2521 atggcggtaa tttccatacg gtcggtgcgg ttctcacgcc gctgtctgtt ccggccttta
     2581 cgttaattgc gggcgaccgc agcatctctg gttctgctac cggcacgcct tatgagctgc
     2641 gaaagctgat gcgctttgcc gcccgcagca aggttgcgcc gacaaccgaa ctgttcccga
     2701 tgtcgaaaat taacgacgcc attcagcatg tacgcgatgg taaagcgcgc taccgagtag
     2761 tcctgaaagc cgacttctga tccctttgcc gggtgctgtc gcccggcact taaatctctt
     2821 tggaaggcgt ctcgcaatct gctgaattta gcggtgaaga gtgcacagcc gttgcctata
     2881 ctccaggcag gaaactggag gaaatctcat gagcgaaccc ctgttaattg cccgcacgcc
     2941 ggacacagaa ctgtttttac tgccgggaat ggctaaccgt cacgggctga ttactggcgc
     3001 aacggggacg ggtaaaaccg ttacgctgca aaaactggca gagtcattgt cggaaatcgg
     3061 cgtgccggtg tttatggctg atgtgaaagg cgatctgacc ggtatcgcgc aggcaggaac
     3121 ggcgtcggaa aaactgctca caaggcttaa aaatatcggc gtcaatgact ggcaaccgca
     3181 tgccaatccg gtggtggtgt gggatatctt tggcgagaaa ggccacacgg tgcgggcgac
     3241 ggtttcggat ctggggccgc tgttgctggc gcgactgttg aatctcaacg atgtgcaatc
     3301 tggcgtgctg aatattatct tccgcattgc ggacgatcag ggattgttgc tgctcgactt
     3361 taaagatttg cgggcgatta cccagtacat cggcgataac gccaaatcct tccagaatca
     3421 gtacggaaat atcagtagcg catcggttgg tgccatccag cgcggattac tgtcgctgga
     3481 acagcaaggc gcagcacact tctttggtga gccgatgctg gatatcaaag actggatgcg
     3541 caccgatgcc aacggtaaag gcgttatcaa tatcctcagc gccgagaaac tttatcagat
     3601 gccgaaactg tacgccgcca gcctgctgtg gatgctttca gagttgtatg aacaattgcc
     3661 ggaagcgggc gatctggaga aaccaaaact ggtgtttttc ttcgacgagg cacatctgct
     3721 gtttaacgat gcaccgcagg tactgctgga taagattgag caggtgataa ggcttattcg
     3781 ctcaaaaggc gtgggcgtct ggttcgtttc gcaaaacccg tctgatattc cggataatgt
     3841 gttcggccag ctcggtaatc gtgttcagca cgctttgcgg gcttttacgc caaaagatca
     3901 gaaagcagtg aagacagcgg cgcaaaccat gcgggccaat ccggcatttg ataccgaaaa
     3961 ggcaatccag gaactgggga ccggcgaggc gttaatctcg tttctcgatg caaaaggaag
     4021 tccttctgtg gtggaacggg cgatggtgat cgcaccttgt tcgcgaatgg ggccggtgac
     4081 ggaagatgag cgtaatggcc tgattaatca ctccccggtg tatggcaaat atgaagatga
     4141 ggtggaccgc gagtccgcct atgagatgct gcaaaaaggc tttcaggcca gtaccgagca
     4201 gcaaaataat ccccccgtga aagggaaaga ggtggcggtg gatgacggta ttcttggtgg
     4261 attgaaggat attttgtttg gcactaccgg accacgcggc gggaagaaag atggtgtggt
     4321 gcaaacaatg gcgaaaagcg ccgctcgcca ggtgacgaat cagattgtac gcgggatgtt
     4381 ggggagtttg ctggggggga gaaaaagata agtcagacgt gatctgtgcc cctcacccta
     4441 accctctccc caaaggggcg aggggaccgt tcgaactcgt atttgacttt gtcaacaata
     4501 ataaagggag ctttcgctcc cttgttagat tacgattttc tcattaacag ccacaggctg
     4561 attaagaaga agctggcact tggcaacagt gcgccgatga tcggcgggat gccgtaaacc
     4621 aacgtcagcg ggccgaagat ctggtccagt acgtagaaga caaaaccgaa gctgatacct
     4681 gtgaccacac gcacgcccat cggtacgcta cgcagtgggc caaagataaa cgacagcgcc
     4741 atcagcatca tcaccgccac ggatagcggc tggaagattt tgctccacat gttgagctga
     4801 taacgtccgg catcctgacc gctcgacttc agatacttca cgtagttgtg caaaccactg
     4861 atggagagtg catccgggtc cagcgccacc acgcccagtt tgtctggcgt gaggtcggtt
     4921 ttccaggtgc cgctcaccgt ctgcgaaccg gtaatctgtt tcggattggt cagatcagat
     4981 tcatcaacct gcgacagacg ccagacttta tgttccgggt caaacttcgc ggtagcggca
     5041 taacgtacgg attgcagacg acgattctcg ttaaaggcat aaatactgat gccacctaac
     5101 acctcgtcac ctttaacccg ctcaatgtag acgaagttgt tgccatcttt cgcccataaa
     5161 ccttgctggg tagagagcaa cgagccgccg tacatcgcct gcgcacggta gttacgcgcc
     5221 atctgttcgc cctgcggcgc gacccattcg ccaatcgcca tcgtcagcaa gaccagtgga
     5281 atggcggttt tcatcaccga cagcgctacc tgcatacggg taaaaccaga agcctgcatc
     5341 accaccagtt cgctgcgctg cgccagcatc ccaagaccaa gcaacgcccc aagaagagcc
     5401 gccatcggga agaagatctg cacatctttc ggcacgctca gcaaggtata cattcctgcg
     5461 cctaacgcgt cgtaactccc ctgcccggct tttttcagct gatcgacaaa tttgataatg
     5521 cccgacagcg acaccagcat gaacagcgtc atcatgatgg tggtgaaaat agttttaccg
     5581 atatagcggt caagtacgcc aaaaggttgc atcacaccgc tcctttacgc gaaaaactgg
     5641 cgcgcaggcg gcggaccggc acggtgtccc aaaggttgag aacaatcgcc aaagccagat
     5701 aaatcaggtt aacggtccac atccacagtg tcgggtccag cttaccttta ccgccgttcg
     5761 atttcaggga ggtctggatc aggaagaaaa gtagatacag cagcatggct ggcagcatcg
     5821 acagtacgcg tccctgacgc gggttaacca cgctcaacgg tacaaccata agtgccatca
     5881 taaacacggt gaataccaac gtgatacgcc agttcagttc tgcgcgagca cgatcggtgt
     5941 cagtgttcca caatgtgcgc atgtccatct ggtcggtatc gttcgggtcg agcgccaccg
     6001 cctggtgacc aatgatcgcc tgataatcct ggaaatccgt aatgcggaaa tcacgtaaca
     6061 gtgcagtgcc ttcgaagcgc gttccctggt tgagagtgac gacctgggag ccgtcgcgca
     6121 gctgggttaa atgtccggaa tcggccacca ccacagaagg acgtgcatta ccttttggtc
     6181 gaatttgtgc gaggaacaca tctttgaaat cgctgccgtc aacgctttcg atgaacagca
     6241 ccgagctgcc attagtcgct tgctggaatt gcccttgcgc cagcgccgcc atgccagggt
     6301 tcgctttcgc ttctgctaac acttcatcct gatgacgcga tgaccacggt cccgcccaca
     6361 tcacgttaac cgccgcgacg attgccgtga ataccgcaag gatcattgcc gctttcacca
     6421 gaaccgcttt gctcaagccg caggcatgca ttaccgtaat ttcactttcg gtatacagtt
     6481 tgcccagcgt catcagcagc ccgaggaaca ggcttaatgg caggataagc tgcgccattt
     6541 ccggcacgcc caacccgaga agggagagca ccagattcgc cggaatatcg ccgtcaaccg
     6601 ctgcgccgag gatcctcact aacttttgac agaagaagat caaaagcaag atgaagagta
     6661 tcgccagctg gcttttgagc gtctcccgca ccagatatct tatgattatc actttaaata
     6721 cgcccgtaaa aactcgtctt ttgcaggatt ttagcttgtt tcatggctta aacgtcattt
     6781 attctcttga gtcgtcgaaa tcgtcgctaa gataattata ctcaacggat tcacctctca
     6841 gattttgttc tgacgtgcca atgccgtaat aacgttaaga ttaacacgaa gtcatcgcaa
     6901 cagcggacat gagttacgaa agcttgcaat tctatctgta gccaccgccg ttgtctttaa
     6961 gattcaggag cgtagtgcat ggagtttagt gtaaaaagcg gtagcccgga gaaacagcgg
     7021 agtgcctgca tcgtcgtggg cgtcttcgaa ccacgtcgcc tttctccgat tgcagaacag
     7081 ctcgataaaa tcagcgatgg gtacatcagc gccctgctac gccggggcga actggaagga
     7141 aaaccggggc agacattgtt gctgcaccat gttccgaatg tactttccga gcgaattctc
     7201 cttattggtt gcggcaaaga acgtgagctg gatgagcgtc agtacaagca ggttattcag
     7261 aaaaccatta atacgctgaa tgatactggc tcgatggaag cggtctgctt tctgactgaa
     7321 ctgcacgtta aaggccgtaa caactactgg aaagtgcgtc aggctgtcga gacagcaaaa
     7381 gagacgctct acagtttcga tcagttgaaa acgaataaga gcgaaccgcg tcgtccgctg
     7441 cgtaaaatgg tgttcaacgt gccgacccgc cgtgaactga ccagcggtga gcgcgcgatc
     7501 cagcacggtc aggcgattgc cgccgggata aaagcagcga aagatctcgg taatatgccg
     7561 ccgaatatct gtaacgccgc ttatctcgcc tctcaggcgc gccagctggc tgacagctac
     7621 agcaagaatg tcatcacccg cgttatcggc gaacagcaga tgaaagagct gggaatgcac
     7681 tcttatctgg cggtcggtca gggttcacaa aacgaatcgc tgatgtcggt gattgagtac
     7741 aaaggcaacg cgtcggaaga tgcacgccca atcgtgctgg tgggtaaagg tttaaccttc
     7801 gactccggcg gtatctccat caagccttca gaaggcatgg atgagatgaa gtacgatatg
     7861 tgcggcgcgg cggcagttta cggcgtgatg cgtatggtcg cggagctgca actgccgatt
     7921 aacgttattg gcgtgctggc aggctgtgaa aacatgcctg gcgggcgtgc ctatcgtccg
     7981 ggcgatgtgt taaccaccat gtccggtcaa accgttgaag tgctgaacac cgatgccgaa
     8041 ggccgcctgg tactgtgcga cgtgttaact tacgttgaac gttttgagcc ggaagcagtg
     8101 attgacgtgg cgacgctgac cggtgcctgc gtgatcgcgc tgggtcatca tattactggt
     8161 ctgatgacga accataatcc gctggcccat gaactgattg ccgcgtctga acaatccggt
     8221 gaccgcgcat ggcgcttacc gctgggtgac gagtatcagg aacagctgga gtccaatttt
     8281 gccgatatgg cgaacattgg cggtcgtcct ggtggggcga ttaccgcagg ttgcttcctg
     8341 tcacgcttta cccgtaagta taactgggcg cacctggata ttgcaggaac cgcctggcgt
     8401 tctggtaaag caaaaggcgc aaccggtcgt ccggtagcgt tgctggcaca gttcctgtta
     8461 aaccgcgctg ggtttaacgg cgaagagtaa gtttgcgttt taccctcacc ctaaccctct
     8521 ccctaaggga gagggaaccg atcgagcgca atagaaattc attgctcatc tttcagggtg
     8581 aggaatgcag caatatttaa atccaccaca agaagcccca tatatgaaaa acgcaacgtt
     8641 ctaccttctg gacaatgaca ccaccgtcga tggcttaagc gccgttgagc aactggtgtg
     8701 tgaaattgcc gcagaacgtt ggcgcagcgg taagcgcgta ctcatcgcct gtgaagatga
     8761 aaagcaggct taccggctgg atgaagccct gtgggcgcgt ccggcagaaa gctttgttcc
     8821 gcataattta gcgggagaag gaccgcgcgg cagtgcgccg gtggagatcg cctggccgca
     8881 aaaacgcagc agcagcccgc gcgatatatt gattagtctg cgaacaagct ttgcagattt
     8941 tgccaccgct ttcacagaag tggtagactt cgttccttat gaagattctc tgaaacaact
     9001 ggcgcgcgaa cgctataaag cctaccgcgt ggctggtttc aacctgaata cggcaacctg
     9061 gaaataatgg aaaagacata caacccacaa gatatcgaac agccgcttta cgagcactgg
     9121 gaaaagcagg gctactttaa gcctaatggc gatgaaagcc aggaaagttt ctgcatcatg
     9181 atcccgccgc cgaacgtcac cggcagtttg catatgggtc acgccttcca gcaaaccatc
     9241 atggatacca tgatccgcta tcagcgcatg cagggcaaaa acaccctgtg gcaggtcggt
     9301 actgaccacg ccgggatcgc tacccagatg gtcgttgagc gcaagattgc cgcagaagaa
     9361 ggtaaaaccc gtcacgacta cggccgcgaa gctttcatcg acaaaatctg ggaatggaaa
     9421 gcggaatctg gcggcaccat tacccgtcag atgcgccgtc tcggcaactc cgtcgactgg
     9481 gagcgtgaac gcttcaccat ggacgaaggc ctgtccaatg cggtgaaaga agttttcgtt
     9541 cgtctgtata aagaagacct gatttaccgt ggcaagcgcc tggtaaactg ggacccgaaa
     9601 ctgcgtaccg ctatttctga cctggaagtg gaaaaccgcg aatcgaaagg ttcgatgtgg
     9661 cacatccgct atccgctggc tgacggcgcg aaaaccgcag acggtaaaga ttatctggtg
     9721 gttgcgacca ctcgcccgga aaccctgctg ggcgataccg gtgtagctgt taacccggaa
     9781 gatccgcgtt acaaagatct gattggcaaa tatgtcattc tgccgctggt taaccgtcgt
     9841 attccgatcg ttggcgacga acacgccgac atggaaaaag gcaccggctg cgtgaagatc
     9901 acaccggcgc acgactttaa cgactatgaa gtcggtaaac gtcacgccct gccgatgatc
     9961 aacatcctga cctttgacgg tgatatccgt gaaagcgccc aggtgttcga taccaaaggt
    10021 aacgaatctg acgtttattc cagcgagatc ccggcagagt tccagaaact cgagcgtttt
    10081 gctgcacgta aagcagtcgt tgctgcggtt gatgcactcg gcctgctgga agaaattaaa
    10141 ccgcacgacc tgaccgttcc ttacggcgac cgtggcggcg tggttatcga accgatgctg
    10201 accgaccagt ggtacgtgcg tgccgatgta ctggcgaaac cggcagttga agcggttgag
    10261 aacggcgaca ttcagttcgt accgaagcag tacgaaaaca tgtacttctc ctggatgcgc
    10321 gatattcagg actggtgtat ctctcgtcag ctgtggtggg gtcaccgtat cccggcatgg
    10381 tatgacgaag cgggtaatgt ttatgttggc cgcaacgaag aagaagtgcg caaggaaaat
    10441 aacctcggtg ccaacgttgc cctgcgtcag gacgaagacg ttctcgacac ctggttctct
    10501 tctgcactgt ggaccttctc tacccttggc tggccggaaa ataccgacgc cctgcgtcag
    10561 ttccacccaa ccagcgtgat ggtatctggt ttcgacatca tcttcttctg gattgcccgc
    10621 atgatcatga tgaccatgca cttcatcaaa gatgaaaatg gcaaaccgca ggtgccgttc
    10681 cacactgttt acatgaccgg tctgattcgt gacgacgaag gtcagaagat gtccaaatcc
    10741 aagggtaacg ttattgaccc gctggatatg gttgacggta tttcgctgcc agaactgctg
    10801 gaaaaacgca ccggcaatat gatgcagccg cagctggcgg acaaaatccg taagcgcacc
    10861 gagaaacagt tcccgaacgg cattgagccg cacggcaccg atgccctgcg cttcaccctg
    10921 gcggcgctgg cgtctaccgg tcgtgacatc aactgggata tgaagcgtct ggaaggctac
    10981 cgtaacttct gtaacaagct gtggaacgcc agccgctttg tgctgatgaa cacagaaggt
    11041 caggattgcg gcttcaacgg cggcgaaatg acgctatcgc tggcggaccg ctggattctg
    11101 gcggagttca accagactat caaagcgtac cgcgaagcgc tggacagctt ccgcttcgat
    11161 atcgccgcag gcattttgta tgagttcacc tggaaccagt tctgcgactg gtatctggag
    11221 ctgaccaagc cggtaatgaa cggtggaaca gaagccgaac tgcgcggtac tcgccatacg
    11281 ctggtgactg tactggaagg tctgctgcgc ctcgcgcatc cgatcattcc gttcatcacc
    11341 gaaaccatct ggcagcgtgt gaaagtactt tgcggtatca ctgccgacac catcatgctg
    11401 cagccgttcc cgcagtatga tgcatctcag gttgatgaag ccgcactggc cgacaccgag
    11461 tggctgaaac aggcgatcgt tgctgtacgt aacatccgtg cagaaatgaa catcgcgccg
    11521 ggcaaaccgc tggagctgct gctgcgtggt tgcagcgcgg atgcagaacg tcgcgtaaat
    11581 gaaaaccgtg gcttcctgca aacgctggcg cgtctggaaa gcatcaccgt gctgcctgcc
    11641 gatgacaaag gtccggtttc cgttaccaag atcatcgacg gcgcagagct gctgatcccg
    11701 atggctggcc tcatcaacaa agaagatgag ctggcgcgcc tggcgaaaga agtggcgaag
    11761 atcgaaggtg aaatcagccg catcgagaac aagctggcaa atgaaggctt tgtcgcccgc
    11821 gcaccggaag cggttatcgc gaaagagcgt gagaagctgg aaggttatgc agaagcgaaa
    11881 gccaagctga ttgaacagca ggctgttatc gccgcactgt aatcaacaca gtgatgaaaa
    11941 cgaaggccgg agcatgctcc ggcctttttt atctcttaca gaaatggtaa agaaggcata
    12001 actccccgag aaatactggc taagaagcct ttatctggtt gttcttcttt attgattaaa
    12061 ggaaggtcat ccaaatcgcc cagcaaatac gtattttttg cctgccagtc aatcatccat
    12121 attttcagca gtgggtaagc cagaccaccc gtaatcccgg atatcaccac caacgcacac
    12181 atgcgataaa gcataccgtg gtacgttaaa gttgagcgaa aacgaatacg cccatcattc
    12241 agtgacaggt tgctcataaa atgatttcgc aacgacaccg ttaaataact ggtgctaaca
    12301 gcaatcccaa aataataaat taactgcgcg attatcattt tacgttgcat ttccataaat
    12361 tgctgtaaat tctcaatatc atcatttgca tacccagaac tatcatactc atttaatatt
    12421 tgatcgaaga taatataacc agcaacagcg agaaaaggta ataaagctaa aaatgcgagg
    12481 atggcatatt ttatacaata cgcagtatct aatttcacct tgaaacgatg tataccgaaa
    12541 ctggtattgc tccagagaaa actcattacc aggctatata aagtaccatt aaaaatacca
    12601 atggaaacaa taccaactat tgccatcaga accacagata taataacact acttgaacta
    12661 ttggcatgta gcatctttgt cgagataaag aaaacagtcc ccatcccaat ggccattaaa
    12721 atcggcaaga aaaaggttac ccaccagaac cctttcaaag agcaattaaa actaaatctt
    12781 acgccgttga gactggtcat taaggcctga taacgcagtc cttttgctgc cataaatata
    12841 agcaaaacca acagtaacaa agtcaaaaca cagccaacaa gcggcatatc tgctgacact
    12901 gtcattaaga ttgcgaaata gaaaaaaaca aaaacaagac aactaacaaa aacattccca
    12961 ccggtaattc cataagaaaa tcgttgtccg ttaacttcca tattagcata aagataacgc
    13021 ttacatttca ttaatgccca tggtaaatag atacctagcg ttataatcgt taacaacaca
    13081 ttcaccacac aaataagaaa atatctctca cctgtaccat gaaaaacaaa agaatgggac
    13141 ggaacatcca tttcattaat aacttgagcc ataaaaatcc cctgaattaa aaaatccatt
    13201 gatataattt atagatacag acagtaaaat gttctgccac gttacaatac tcttatcagt
    13261 aataggctgg caaaaacttt attgctttat aagacgcttg cgcacgtaag aagagagtgg
    13321 tattaaacaa atgtataatt actttatttt tattgggtta aactatgaat aacattgcgc
    13381 cgcaatcacc tgtaatgcgt cgcctgacgc tgcaggataa tcctgctatc gccagcgtca
    13441 tccgtcaggt ttacgcagag tatggtctta ccgccgataa aggctacacc gttgccgatc
    13501 cgaatctcga cgaactgtat caagtgtaca gccagcctgg ccatgcttat tgggtggttg
    13561 aatacaatgg cgaagtggtc ggcggcggcg ggattgcgcc attaacaggt agtgaagcgg
    13621 acatttgcga actgcagaaa atgtactttc ttcccgctat tcgcggtaaa gggctagcga
    13681 aaaaactggc cttaatggcg atggagcagg cacgagagat gggcttcaaa cgctgctatc
    13741 tggaaacgac cgctttttta aaggaagcca ttgcgcttta tgagcatttg ggctttgagc
    13801 atatcgacta tgcgcttggc tgcacgggcc atgtcgattg tgaagtgcgg atgctacgcg
    13861 aactctaaaa aaatgcccgc tagtatgtgc tatcgggcat tttaatcaat taattagtgg
    13921 cgaaccccgt catcgtcttc atcgacaaaa tcttcatcgt cgccatcttc gccgttagga
    13981 tcttcaaagt aagtgcccca accgtcgtac tcaacgtcaa atttctccgc cagcgtcatc
    14041 agttgttcaa cctgggcatc gatcagatcg gcattcaacg cgcactcgct gaggatgtcg
    14101 cagcaaatca cgatatcacc atcttcaact tccagctctt ctggatcggt cacttcgtaa
    14161 ccgagtttaa acgcttcaac tgccgctttt tccagagttt ccagatcgtc tgcggaaaga
    14221 tggtgttcga tggtgtacag cgcgtccgga tcgctgccat cttccagtaa ttcttcaata
    14281 atcaaacgtg tttcttcacg ctgttcttcc agttgttccg ggtttgccat ggctcattcc
    14341 tcttaatgtc ctgccgatac tcttattgtc acacaccact gacattgcct ccacctttgt
    14401 gacaaagatt tatgctttag acttgcaaat gaataataat acacataaat tgagttttaa
    14461 ttcattgagg cgttagccac aggagggatc tatgtccggg ttttatcata agcatttcct
    14521 gaaattactc gatttcacgc cagctgaact caacagcctg ctgcagttag ccgcgaagct
    14581 gaaagctgat aagaaaagcg gtaaagaaga agccaaactc actggtaaaa acatcgcgct
    14641 catcttcgaa aaagactcga ctcgtacccg atgctctttc gaagttgccg catatgacca
    14701 gggggctcgc gttacttatc ttggcccaag cggtagtcag attggtcata aagagtcgat
    14761 taaagacact gcccgcgtgc ttggtcgcat gtatgacggt attcagtatc gcggctatgg
    14821 tcaggagatt gtcgaaacac tggcggaata cgctggcgtg ccggtatgga acggcctgac
    14881 caatgagttt caccccacgc agttgctggc ggatctcctc accatgcagg agcatttacc
    14941 cggtaaagca ttcaacgaaa tgacgctggt ctatgcaggt gacgcacgta acaacatggg
    15001 caattcgatg ctcgaagctg cggcgcttac cggtctggat ttgcgtctgg tcgcgccaca
    15061 ggcatgctgg ccagaagctg cgctggttac ggaatgccgc gccctggcac aacaaaatgg
    15121 cgggaatatt acgctgacgg aagatgtagc taaaggagtt gaaggtgctg actttatcta
    15181 taccgatgta tgggtgtcga tgggtgaagc aaaagaaaaa tgggctgaac gcattgcatt
    15241 actgcgtgat tatcaggtga acagcaagat gatgcagttg accggtaacc cggaggtcaa
    15301 attcctccat tgcctgcccg cgtttcatga cgaccaaacg acgcttggca aaaaaatggc
    15361 ggaagagttt ggcctacatg gcggaatgga agtgactgat gaggtcttcg aatctgccgc
    15421 cagcattgta tttgatcagg cggaaaaccg catgcatacc atcaaagcgg tgatggtcgc
    15481 gacgctcagt aaattaaata attaatatct ctataaa
//
LOCUS       DAJTRC010000086        15344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.086, whole genome shotgun sequence.
ACCESSION   DAJTRC010000086 DAJTRC010000000
VERSION     DAJTRC010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.086"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(173..1198)
                     /gene="tdh"
                     /locus_tag="OO357_003013"
     CDS             complement(173..1198)
                     /gene="tdh"
                     /locus_tag="OO357_003013"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HCR5887878.1"
                     /translation="MKALSKLKAEEGIWMTDVPVPELGHNDLLIKIRKTAICGTDVHI
                     YNWDEWSQKTIPVPMVVGHEYVGEVVGIGQEVKGFKIGDRVSGEGHITCGHCRNCRGG
                     RTHLCRNTIGVGVNRPGCFAEYLVIPAFNAFKIPDNISDDLASIFDPFGNAVHTALSF
                     DLVGEDVLVSGAGPIGIMAAAVAKHVGARNVVITDVNEYRLELARKMGITRAVNVAKE
                     NLNDVMAELGMTEGFDVGLEMSGAPPAFRTMLDTMNHGGRIAMLGIPPSDMSIDWTKV
                     IFKGLFIKGIYGREMFETWYKMAALIQSGLDLSPIITHRFSIDDFQKGFDAMRSGQSG
                     KVILSWD"
     gene            complement(1208..2404)
                     /gene="kbl"
                     /locus_tag="OO357_003014"
     CDS             complement(1208..2404)
                     /gene="kbl"
                     /locus_tag="OO357_003014"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020239151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HCR5887879.1"
                     /translation="MRGEFYQQLTNDLETARAEGLFKEERIITSAQQADITVADGSHV
                     INFCANNYLGLANHPDLIAAAKAGMDSHGFGMASVRFICGTQDSHKELEQKLAAFLGM
                     EDAILYSSCFDANGGLFETLLGAEDAIISDALNHASIIDGVRLCKAKRYRYANNDMQE
                     LEARLKEAREAGARHVLIATDGVFSMDGVIANLKGVCDLADKYDALVMVDDSHAVGFV
                     GENGRGSHEYCDVMGRVDIITGTLGKALGGASGGYTAARKEVVEWLRQRSRPYLFSNS
                     LAPAIVAASIKVLEMVEAGSELRDRLWANARQFREQMSAAGFTLAGADHAIIPVMLGD
                     AVVAQKFARELQKEGIYVTGFFYPVVPKGQARIRTQMSAAHTPEQITRAVEAFTRIGK
                     QLGVIA"
     gene            complement(2679..3536)
                     /gene="yibB"
                     /locus_tag="OO357_003015"
     CDS             complement(2679..3536)
                     /gene="yibB"
                     /locus_tag="OO357_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YibB"
                     /protein_id="HCR5887880.1"
                     /translation="MKSSTTIITAYFDIGRGDWTANKGFREKLARSVDVYFSYFERLA
                     ALENEMIIFTSPDLKPRIEAIRNGKPTTVIVIDIKKKFRYIRSRIEKIQKDESFTNRL
                     EPRQLKNPEYWSPEYVLVCNLKAYFVNKAINMGLVKTPLVAWIDFGYCRKPNVTRGLK
                     IWDFPFDENKMHLFTIKKGLTVTSQQQAFDFMIGNHVYIIGGAIVGSQHKWKEFYKLV
                     LESQKITLNNNIVDDDQGIFVMCYYKRSDLFNLNYLGRGKWFDLFRCFRSNTLGAKMQ
                     ALRIFLSRK"
     gene            3840..4772
                     /gene="rfaD"
                     /locus_tag="OO357_003016"
     CDS             3840..4772
                     /gene="rfaD"
                     /locus_tag="OO357_003016"
                     /EC_number="5.1.3.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020233050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-glyceromanno-heptose 6-epimerase"
                     /protein_id="HCR5887881.1"
                     /translation="MIIVTGGAGFIGSNIVKALNDKGITDILVVDNLKDGTKFVNLVD
                     LDIADYMDKEDFLIQIMAGEEFGDVEAIFHEGACSSTTEWDGKYMMDNNYQYSKELLH
                     YCLEREIPFLYASSAATYGGRTSDFIESREYEKPLNVYGYSKFLFDEYVRQILPEANS
                     QIVGFRYFNVYGPREGHKGSMASVAFHLNTQLNNGESPKLFEGSENFKRDFVYVGDVA
                     DVNLWFLENGVSGIFNLGTGRAESFQAVADATLAYHKKGQIEYIPFPDKLKGRYQAFT
                     QADLTNLRAAGYDKPFKTVAEGVTEYMAWLNRDA"
     gene            4782..5828
                     /gene="rfaF"
                     /locus_tag="OO357_003017"
     CDS             4782..5828
                     /gene="rfaF"
                     /locus_tag="OO357_003017"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-heptose--LPS heptosyltransferase RfaF"
                     /protein_id="HCR5887882.1"
                     /translation="MKILVIGPSWVGDMMMSQSLYRTLQARYPQAIIDVMAPAWCRPL
                     LSRMPEVNEAIPMPLGHGALEIGERRKLGHSLREKRYDRAYVLPNSFKSALVPFFAGI
                     PHRTGWRGEMRYGLLNDVRVLDKEAWPLMVERYVALAYDKGIMRTAQDLPQPLLWPQL
                     QVSEGEKSYTCNQFSLSSERPMIGFCPGAEFGPAKRWPHYHYAELAKQLIDEGYQVVL
                     FGSAKDHEAGNEILAALNTEQQAWCRNLAGETQLDQAVILIAACKAIVTNDSGLMHVA
                     AALNRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDAAEGYHQSLIDITPQR
                     VLEELNALLLQEEA"
     gene            5832..6824
                     /gene="rfaC"
                     /locus_tag="OO357_003018"
     CDS             5832..6824
                     /gene="rfaC"
                     /locus_tag="OO357_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide heptosyltransferase RfaC"
                     /protein_id="HCR5887883.1"
                     /translation="MRVLIVKTSSMGDVLHTLPALTDAQQAIPGIKFDWVVEEGFAQI
                     PSWHAAVERVIPVAIRRWRKAWFSAPIKAERKAFREALQAENYDAVIDAQGLVKSAAL
                     VTRLAHGVKHGLDWQTAREPLASLFYNCKHHIAKQQHAVERTRELFAKSLGYSKPQTQ
                     GDYAIAQHFLTNLPTDAGEYAVFLHATTRDDKHWPEEHWRELIGLLADSGIRIKLPWG
                     APHEEERAKRLAEGFAYVEVLPKMSLEGVARVLAGAKFVVSVDTGLSHLTAALDRPNI
                     TVYGPTDPGLIGGYGKNQVECRSTSMSLADLPAQTVFQNLNLEIITNKLTSEIR"
     gene            6821..8029
                     /gene="rfaL"
                     /locus_tag="OO357_003019"
     CDS             6821..8029
                     /gene="rfaL"
                     /locus_tag="OO357_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase RfaL"
                     /protein_id="HCR5887884.1"
                     /translation="MTSTLFFSLEKKNWIAYWNRALVFLFITTYFLGGITRYKHLIVI
                     LMTITTIVYLCKRPKHYLSLFKTFLFGSVAILTIAALLSLLQSPDAGASMKEVFKAII
                     ENTLLCTIAIPVILRDEKREDVEKIVFFSFISALGLRCFSELITYYKDYQQGIMPFAD
                     YRHRSISDSMVFLFPALLNLWLIKSAKYRISFVVLSVIFIFLILGTLSRGAWLSVLVI
                     GLIWILMFKQWKLLLVGVMVSIIALSVIFTHKEMTAKLTYKLQQTNSSYRYANGTQGS
                     ALDLILENPVIGYGYGNVAYKDVYNKRVIDYPEWTFRQSIGPHNFALFIWFGTGLLGL
                     VSLMMLYCAILKECIKNGVKNKYRSPYNAYYIILLSFIGYFVIRGNVEQIEPNLLGVY
                     AGLLLAMKNK"
     gene            complement(8065..9207)
                     /locus_tag="OO357_003020"
     CDS             complement(8065..9207)
                     /locus_tag="OO357_003020"
                     /EC_number="2.4.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     N-acetylglucosaminyltransferase"
                     /protein_id="HCR5887885.1"
                     /translation="MVDKIIFTVTPIFSIPPRGAAAVETWMYQVAQRTNFPNRIVCIK
                     NPGYSNYTFVNDNCSIHRVGFSRIYKRLFQKWTRLDPLPYSQRILNIAHDFPITKESV
                     IVVHNSMKLYTQIRKLAPQARVVIHMHNAFEPKLLEQNVKMIVPSLYLKKYYQSYLAN
                     ADIEIVPNGIDLETYQSNFQPITRSELNISPEEKIIFYAGRIVPDKGILLLMQSFEKL
                     AAAHKNLKLVVIGDYTEMSKSDKGAYQRNVREIAKRLKDRCIMLGSIPPEKMYCYYPL
                     ADLVVIPSQFQEPFCMVAIEAMGAGKPVLVSTRGGMTEFVKENTTGFHLKEPMTADSI
                     SSDILKTLANPELTAVAKQGQDFVFDHYSWDGVTQRFEEVIHNWFE"
     gene            complement(9216..10229)
                     /locus_tag="OO357_003021"
     CDS             complement(9216..10229)
                     /locus_tag="OO357_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--(galactosyl) LPS
                     alpha1,2-glucosyltransferase"
                     /protein_id="HCR5887886.1"
                     /translation="MDFKHLTQFKDIIELDKRPVKLDERETFNVSWGIDENYQVGAAI
                     SIASILEKNKQNKFTFHIIADYLDKEYIELLSQLATKYQTVIKLYHIDSEPLKALPQS
                     NIWPVSIYYRLLSFDYFSARLDSLLYLDADIVCKGSLNELIALEFKDEYGAVVIDVDA
                     MQSKSAERLCNEDFNGSYFNSGVMYINLREWLKQRLTEKFFDLLSDESIIKKLKYPDQ
                     DILNLMFLHHAKILPRKYNCIYTIKSEFEEKNSEYYTRFINDDTVFIHYTGITKPWHD
                     WANYASADYFRNIYNISPWRNIPYKKAVKKHEHKEKYKHLLYQKKFLDGVFTAIKYNV
                     MKG"
     gene            complement(10254..10961)
                     /gene="rfaY"
                     /locus_tag="OO357_003022"
     CDS             complement(10254..10961)
                     /gene="rfaY"
                     /locus_tag="OO357_003022"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(II) kinase RfaY"
                     /protein_id="HCR5887887.1"
                     /translation="MIYNKTINGLKVFIKDNDPFYEQVLNDFLTCRVKTLKVFRSIDD
                     TKVILIDTARGPLVLKVYAPKHKMTERFLKSCIKKDYYENLIYQTDRVRGEGIQSIND
                     YFLLAERKTLNFAHYYIMLIEYIEGVGLNEYLEISEDLKDQLSESIKELHQHGMVSGD
                     PHKGNFIVSEKGLRLIDLSGKKTTAVLKAKDRIDLERHYNIKNELKDFGYTYLIFKKK
                     IKKVIRDVKVKLGLKSK"
     gene            complement(10987..11994)
                     /gene="waaO"
                     /locus_tag="OO357_003023"
                     /gene_synonym="rfaI"
     CDS             complement(10987..11994)
                     /gene="waaO"
                     /locus_tag="OO357_003023"
                     /gene_synonym="rfaI"
                     /EC_number="2.4.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide
                     3-alpha-galactosyltransferase"
                     /protein_id="HCR5887888.1"
                     /translation="MSQLNDSDIILFEYNFHYQNIRSKNTLDIAFGIDRNFLFGCGVA
                     IASILLNNREISCEFHIFTDYISDKDKLYFSDLAKQYNSRINIYVINCDKLKSLPSTK
                     NWTYATYFRFIIADYFYHKHEKILYLDADIACKGSIKELLDYQFSTNEIAAVVAERDI
                     EWWQNRASVLTTPQLASGYFNAGFLLINIDEWNLNNISSKAIEMLRDPDWVSKITHLD
                     QDVLNVLLNGKVKFISEKYNTRYSINYELKDKVDNPVNDDTVFIHYVGPTKPWHEWAD
                     YPVSRSFLIAKAASPWSKEDLLKPVNSNQYRYCAKHKFKQKHYMAGIFNYLKYYKEKC
                     F"
     gene            complement(12037..12834)
                     /gene="rfaP"
                     /locus_tag="OO357_003024"
     CDS             complement(12037..12834)
                     /gene="rfaP"
                     /locus_tag="OO357_003024"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312532.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptose(I) kinase RfaP"
                     /protein_id="HCR5887889.1"
                     /translation="MVELKEPFATLWRGKDPFEEVKTLQGEVFRELETRRTLRFEMAG
                     KSYFLKWHRGTTLKEIIKNLLSLRMPVLGADREWNAIHRLRDVGVDTMYGVAFGEKGM
                     NPLTRTSFIITEDLTPTISLEDYCADWATNPPDVRVKRMLIKRVATMVRDMHAAGINH
                     RDCYICHFLLHLPFSGKEEELKISVIDLHRAQLRTRVPGRWRDKDLIGLYFSSMNIGL
                     TQRDIWRFMKVYFAAPLKDILKQEQGLLSQAEAKATKIRERTIRKSL"
     gene            complement(12827..13951)
                     /locus_tag="OO357_003025"
     CDS             complement(12827..13951)
                     /locus_tag="OO357_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HCR5887890.1"
                     /translation="MIVAFCLYKYFPFGGLQRDFMRIAQTVAARGHHVRVYTQSWEGE
                     CPDVFEVIKVPVKSHTNHGRNAEYFAWVQKHLREHPVDKVVGFNKMPGLDVYYAADVC
                     YAEKVAQEKGFFYRLTSRYRHYAAFERATFEQGKPTQLLMLTDKQIADFQKHYQTEAE
                     RFHILPPGIYPDRKYSQQPANSREIFRKKNGITEQQYLLLQVGSDFTRKGADRSIEAL
                     ASLPDSLRHNTLLYVVGQDKPRKFEALAEKRGVRSNVHFFSGRNDVSELMAAADLLLH
                     PAYQEAAGIVLLEAITAGLPVLTTAVCGYAHYIVDANCGEAIAEPFRQETLNEILRKA
                     LTQSSLRQAWAENARHYADTQDLYSLPEKAADIITGGLDG"
     gene            complement(13948..15006)
                     /gene="rfaQ"
                     /locus_tag="OO357_003026"
     CDS             complement(13948..15006)
                     /gene="rfaQ"
                     /locus_tag="OO357_003026"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide core heptosyltransferase
                     RfaQ"
                     /protein_id="HCR5887891.1"
                     /translation="MDKPFRRILLIKMRFHGDMLLTTPVISSLKKNYPDAKIDVLLYQ
                     DTIPILSENPEINALYGIKNKKAKASEKIANFFHLIKVLRANKYDLIVNLTDQWMVAI
                     LVRLLNARVKISQDYHHRQSAFWRKSFTHLVPLQGGNVVESNLSVLTPLGLDSLVKQT
                     TMSYPPASWKRMRRELDHAGVGQNYVVIQPTARQIFKCWDNAKFSAVIDALHARGYEV
                     VLTSGPDKDDLACVNEIAQGCQTPPVTALAGKVTFPELGALIDHAQLFIGVDSAPAHI
                     AAAVNTPLISLFGATDHIFWRPWSNNMIQFWAGDYREMPTRDQRDRNEMYLSVIPAAD
                     VIAAVDKLLPSSTTGTSL"
BASE COUNT         4380 a         3445 c         3145 g         4374 t
ORIGIN      
        1 ctgtgagata ttatttttac aggtgagaga gtcacttcag ggtagagaga taatgagaac
       61 agctatataa aagatcagcg caattacgaa ctctttatta cactgacagg attttacata
      121 attaacagat tatcctcaga taaaagggct ggaataccag cccttgttcg tgttaatccc
      181 agctcagaat aactttcccg gactggcccg aacgcatagc gtcaaagccc ttctggaaat
      241 catcgataga gaaacgatgg gtaatgatcg gcgagagatc gaggccagac tgaatcagcg
      301 ccgccatctt gtaccaggtt tcaaacatct cacgaccgta aatgccttta atgaacaagc
      361 ctttaaagat cactttggtc cagtcgatag acatatcaga cggtggaata cccagcatcg
      421 caatacgacc gccgtggttc atggtatcga gcatggtacg aaacgctggc ggcgcgccgg
      481 acatttccag accgacatca aagccttcgg tcatgcccag ttcagccatc acatcattaa
      541 ggttttcttt cgcgacgtta accgcacggg tgatacccat tttgcgcgcc agctcaaggc
      601 ggtattcgtt aacatcggtg atcaccacat tgcgtgcacc aacgtgtttc gccaccgccg
      661 cagccataat acctatcgga cctgcaccag aaaccagcac atcttcgccc accagatcga
      721 acgacagcgc ggtatgcacg gcgttaccga agggatcaaa aatggaagcc aggtcgtcgg
      781 agatattgtc ggggattttg aaagcgttga acgccggaat caccagatat tcggcgaagc
      841 agcccggacg gttaacaccg acaccaatgg tgttgcggca cagatgggta cgaccgccac
      901 gacagttgcg acagtgaccg caggtgatat ggccttcgcc agaaacgcga tcgccgattt
      961 tgaagccttt cacttcctga ccaataccta ccacttcacc gacatattca tggcccacga
     1021 ccatcggcac cgggatggtt ttttgcgacc actcatccca gttatagatg tgaacgtcag
     1081 tcccgcagat ggctgtttta cggattttaa tcagcagatc gttatgcccg agttccggta
     1141 cagggacgtc ggtcatccag atgccctctt ccgctttcag tttggataac gctttcatct
     1201 cacatcctca ggcgataacg cccagttgtt taccaatacg cgtgaatgct tctactgcac
     1261 gcgtaatttg ctcaggggta tgcgccgcag acatctgggt acgaatacgc gcctgacctt
     1321 tcggaacgac cggatagaag aaaccggtga cgtaaatgcc ctctttttgc agctctcggg
     1381 caaatttctg cgccactacc gcatcaccaa gcatgaccgg aataatggcg tgatcggctc
     1441 ccgccagggt aaagcccgcc gccgacattt gctcacggaa ctgacgcgcg ttcgcccaca
     1501 gacggtcacg caactcgcta cccgcttcaa ccatctccag cactttgatg gaagcggcga
     1561 caattgctgg tgccagcgag ttggagaaca ggtacggacg agaacgctgg cgcagccact
     1621 caaccacttc tttgcgcgcc gcggtataac caccagaagc cccgcccagc gctttaccga
     1681 gcgtgccggt gatgatgtcg acacgcccca tcacatcgca gtattcatgg gaaccacgac
     1741 cattttcacc gacaaaaccg accgcgtggg agtcgtctac catcaccagg gcatcgtatt
     1801 tatctgccag atcgcaaacg cccttcagat tggcaatcac gccgtccatc gagaacacac
     1861 catcggtggc aatcagcaca tgacgcgcac cggcttcacg cgcttctttc agacgagctt
     1921 caagctcctg catatcgttg ttggcatagc gatagcgttt agctttacac agacgcacac
     1981 catcaataat agacgcgtgg ttcagtgcgt cggagataat ggcgtcttcc gcgcccagca
     2041 gcgtttcaaa caggccaccg ttagcatcaa agcaggaaga gtagagaatc gcatcttcca
     2101 tccccaggaa tgccgccagt ttttgttcaa gctctttatg gctgtcctga gtgccgcaaa
     2161 taaaacgcac cgaagccatg ccgaaaccgt gagaatccat tcccgccttt gctgccgcaa
     2221 tcagatccgg atgattcgcc agcccgagat agttgttggc acaaaagtta atgacgtggc
     2281 ttccatcagc cacggtgata tctgcttgct gcgcagacgt aataatgcgc tcttctttaa
     2341 acaacccttc cgcccgtgcg gtttccagat cgttggttaa ctgctgataa aattctccac
     2401 gcattgcgat tctccagaca gggcaaattc cagcacatat tacccaaact tataggtcgc
     2461 gacgagatga tgcgttaaca cttctgcaaa attcaggata aataacgtgt atcttgatgt
     2521 ctctcaacac ctacagatag tagaaaaact gtacagtttt ggctgagcat aatgatgagt
     2581 gattttactg cattgatata aaaagaaaaa atgatgccaa agcatcattt ttcctatctc
     2641 agttggcatc aaaacgctac ccactaatgg agggaaacct attttcttga aaggaaaatt
     2701 ctcagcgcct gcatttttgc ccctaaagtg ttactcctga agcagcgaaa caaatcgaac
     2761 cattttcctc tccccagata gtttaggttg aagagatcgg aacgtttgta atagcacatg
     2821 acaaaaatgc cctggtcatc gtccacaatg ttattgttta gtgttatttt ttgactttcc
     2881 agaactaatt tatagaattc tttccattta tgctgtgaac caacaattgc gcccccaata
     2941 atatatacat gattgccaat catgaaatca aaagcctgtt gttgagatgt aacagtaagc
     3001 ccttttttta tagtgaaaag atgcatctta ttttcatcaa atgggaaatc ccaaattttc
     3061 aatcctcgag ttacatttgg cttacggcaa tagccaaaat ctatccacgc aacaagaggc
     3121 gttttcacta aacccatgtt gatagcttta tttacaaaat aggccttaag gttacatacc
     3181 aaaacatact ctggtgacca gtactctggg ttttttaatt gtcgaggctc aagtctattt
     3241 gtaaatgact catctttttg aattttttcg atacgacttc tgatatatct aaatttcttt
     3301 tttatatcga taacgataac cgttgttggt tttccgttgc gaatcgcttc aatccttggt
     3361 ttcaaatcgg gagaagtgaa aataatcatc tcattttcaa gtgcggccag acgctcaaag
     3421 taactaaaat aaacatcaac tgaacgagct agtttttcac ggaacccttt attagcagtc
     3481 caatcccctc taccaatgtc aaaatatgca gtgataattg tggtggatga tttcattact
     3541 aataagacca ttaatataaa aaaagaatta tatcataaat attaacagaa tgtatactgt
     3601 atattatcca ttcaaatgat tccgttattt atcatacatc ggaatattga tactaaagca
     3661 ctattcacat gcaaaaccaa catccgccat gaaggactag ctaaaaccca aactagtttg
     3721 ttgcaattag catccttgca cctctatgta aagggctgaa gggattcgga tgtgatggta
     3781 tgattacaga cattcgtgtc tgagattgtc tctgactcca taattcgaag gttacagtta
     3841 tgatcatcgt taccggcggc gcgggcttta tcggcagcaa catcgttaag gccctgaatg
     3901 ataaaggcat caccgatatt ctggtggtgg acaacctgaa agacggcacc aagtttgtga
     3961 acctggtgga tctggatatc gcggactata tggataagga agacttcctg atccagatta
     4021 tggctggcga agagttcggt gatgtcgaag cgattttcca cgaaggcgca tgctcttcca
     4081 ccaccgagtg ggatggcaag tatatgatgg ataacaacta tcaatactcc aaagagctgc
     4141 tgcactactg tctggagcgc gaaatcccgt tcctgtatgc ctcttccgca gccacctacg
     4201 gcggacgcac ctccgacttt attgaatccc gcgagtacga aaaaccgttg aatgtctacg
     4261 gttactcaaa attcctgttt gatgaatatg ttcgtcaaat cctgccagaa gcgaactcgc
     4321 agattgttgg cttccgctat ttcaacgttt atggaccgcg tgaaggccat aaaggcagca
     4381 tggcgagcgt cgctttccat ctcaacaccc agcttaataa cggtgaatca ccgaagctgt
     4441 tcgaaggtag cgagaacttc aaacgtgact tcgtctatgt gggcgacgtg gcagatgtaa
     4501 acctgtggtt cctggaaaat ggcgtttccg gtatcttcaa cctcggcacc ggtcgtgcag
     4561 aatccttcca ggcagtagca gatgctacgc tggcttatca caagaaaggc cagatcgaat
     4621 acattccgtt cccggataag ctgaaaggcc gctaccaggc gttcactcag gcagatctga
     4681 caaatctgcg cgcggcgggt tacgacaaac cgttcaaaac cgttgctgaa ggtgtaacgg
     4741 aatacatggc ctggctgaat cgcgacgcat aagagctctg catgaaaata ctggtgatcg
     4801 gcccgtcttg ggttggcgac atgatgatgt cgcaaagtct ctatcgcacg ctccaggcgc
     4861 gctatcccca ggcgataatc gatgtgatgg caccggcatg gtgccgtcca ttattatcgc
     4921 ggatgccgga agttaacgaa gctatcccta tgcctctcgg tcacggagcg ctggaaatcg
     4981 gcgaacgccg caaactgggt catagcctgc gtgaaaagcg ctacgaccgc gcctacgtct
     5041 taccaaactc cttcaaatct gcattagtgc ctttcttcgc gggtattcct catcgcaccg
     5101 gctggcgcgg cgagatgcgc tacggtttac tcaacgatgt acgcgtgctc gataaagaag
     5161 cctggccgct aatggtggaa cgctatgtcg cgctggccta tgacaaaggc attatgcgta
     5221 ccgcacaaga tctgccgcag ccattgttat ggccgcagtt gcaggtgagc gaaggtgaaa
     5281 aatcatatac ctgtaatcaa ttttcgcttt catcagaacg tccgatgatt ggcttttgcc
     5341 cgggtgcgga gtttggtccg gcaaaacgct ggccacacta ccactatgcg gaactggcaa
     5401 agcagctgat tgatgaaggt tatcaggtgg ttctgtttgg ctctgcgaaa gatcatgaag
     5461 cgggcaatga gattcttgcc gctttgaata ccgagcagca ggcatggtgt cggaacctgg
     5521 cgggggaaac acagcttgat caagcggtta tcttgattgc agcctgtaaa gccattgtca
     5581 ctaacgattc tggcctgatg cacgttgcgg cggcgctcaa tcgtccgctg gttgccctgt
     5641 atggtccaag tagcccggac ttcacaccgc cgctatccca taaagcgcgc gtgatccgtc
     5701 tgattaccgg ctatcacaaa gtgcgtaaag gtgacgctgc ggagggttat caccagagct
     5761 tgatcgacat tactccccag cgcgtactgg aagaactcaa cgcgctattg ttacaagagg
     5821 aagcctgacg gatgcgggtt ttgatcgtta aaacatcgtc gatgggcgat gttctccata
     5881 cgttgcccgc actcactgat gcccagcagg caatcccagg gattaagttt gactgggtgg
     5941 tggaagaagg gttcgcacag attccttcct ggcacgctgc cgttgagcga gttattcctg
     6001 tggcaatacg tcgctggcgt aaagcctggt tctcggcccc cataaaagct gaacgcaaag
     6061 cgtttcgtga agcgctacaa gcagagaact atgacgcagt tatcgacgct caggggctgg
     6121 taaaaagcgc ggcactggtg acacgtctgg cgcatggcgt aaagcatgga ttggactggc
     6181 aaaccgctcg cgaaccttta gccagcctgt tttacaattg taagcatcat attgcaaaac
     6241 agcagcacgc cgtagaacgc acccgcgaac tgtttgccaa aagtttgggc tatagcaaac
     6301 cgcaaaccca gggcgattat gctatcgcac agcattttct gacgaacctg cctacagatg
     6361 ctggcgaata tgccgtattt cttcatgcga cgacccgtga tgataaacac tggccggaag
     6421 aacactggcg agaattgatt ggtttactgg ctgattcagg aatacggatt aaacttccgt
     6481 ggggcgcgcc gcatgaggaa gaacgggcga aacgactggc ggaaggattt gcttatgttg
     6541 aagtattgcc gaagatgagt ctggaaggcg ttgcccgcgt gctggccggg gctaaatttg
     6601 tagtgtcggt ggatacgggg ttaagccatt taacggcggc actggataga cccaatatca
     6661 cggtttatgg accaaccgat ccgggattaa ttggtgggta tgggaagaat caagtagagt
     6721 gtcgttctac aagtatgtct cttgcagatt tgccagctca aaccgttttt caaaatttaa
     6781 atcttgaaat aataaccaat aagttgacat cggagataag atgacctcaa cattattttt
     6841 ctctctcgag aaaaaaaact ggatagcgta ctggaacaga gctctcgtat tcttattcat
     6901 taccacctat tttttgggtg ggattacaag gtataaacat cttattgtta ttcttatgac
     6961 aataacgaca atcgtctatc tctgcaaacg gccaaaacac tatctctcac tatttaaaac
     7021 atttcttttt ggtagtgttg ccatattaac tattgctgca ttgctgtcac ttcttcaatc
     7081 ccctgatgca ggtgctagca tgaaggaagt tttcaaagct attattgaga atactttact
     7141 atgcacaata gcaataccgg tcatattgag agacgagaaa agagaagatg tcgaaaaaat
     7201 cgttttcttc tcatttatta gtgcgttggg cttacgctgt ttttctgaat tgattaccta
     7261 ttataaggac tatcaacaag ggataatgcc attcgcagat tatagacacc gtagcatttc
     7321 tgactcgatg gtctttttat tccctgcatt gttaaatctc tggcttatca aatcagcaaa
     7381 ataccgcatt tcttttgtgg ttctaagcgt tatttttatt tttctgatat taggaacttt
     7441 atccagaggg gcctggcttt ccgtgttagt cattggatta atatggattc tgatgtttaa
     7501 acaatggaag ttactattag taggagtaat ggtttccatc attgcattgt cggttatttt
     7561 cacacataag gagatgaccg caaagctaac gtataaactt caacaaacta atagttctta
     7621 tcgctatgca aatggtactc aaggcagcgc actcgatcta atattagaaa atcctgttat
     7681 tggttatggt tacggtaacg ttgcatataa agatgtctat aataaacgtg tcattgatta
     7741 tccagaatgg acctttagac aatcaatagg gccacataat tttgcgctat tcatctggtt
     7801 tggcactggt ttattagggc tggtaagtct tatgatgcta tactgtgcaa tattgaaaga
     7861 gtgtataaaa aatggcgtca agaataaata tcgctcacca tataatgcat attatataat
     7921 cttactatct tttataggtt attttgttat ccgtggaaac gtagaacaaa ttgaaccaaa
     7981 tttattaggc gtttacgccg gcttattatt agcgatgaaa aacaagtaaa aaataaaaaa
     8041 ggctgcataa tgcggccttt ttatttattc aaaccaatta tgaataacct cttcgaatct
     8101 ttgagttacg ccatcccaac tgtagtgatc aaacacaaag tcctgacctt gctttgcaac
     8161 tgctgttaat tcaggattcg ctaacgtttt taaaatatcg ctgcttatgg agtctgcagt
     8221 cataggttct tttaaatgga atcccgtagt attttctttg acaaattccg tcatcccccc
     8281 acgcgtactc actaaaacag gcttaccagc acccatagct tcaattgcca ccatgcaaaa
     8341 tggttcctgg aactgggaag gaatgacgac taaatctgcc agtgggtaat aacaatacat
     8401 cttttcaggc ggtatgcttc ctaacataat gcaacgatcc ttcaaacgtt ttgcaatttc
     8461 cctgacattg cgttgatatg cacctttgtc acttttactc atttctgtat aatcaccaat
     8521 cacaactaat tttaaatttt tgtgagcagc ggccaatttt tcgaatgatt gcatcaacag
     8581 caatatgcct ttgtcaggga cgatacgccc agcataaaaa ataattttct cttcaggaga
     8641 gatattaagt tcgcttctag tgatcggctg aaaattagat tgataggttt ctaaatcaat
     8701 tccatttggt acaatctcaa tatctgcatt tgctaaataa gattggtaat actttttcaa
     8761 gtataaacta gggactatca ttttaacatt ttgctcaagc aattttggtt caaatgcatt
     8821 atgcatatgt ataaccactc ttgcctgagg agctagctta cgaatttgag tgtatagctt
     8881 catgctatta tgaactacaa taacactttc tttcgttata ggaaaatcat gtgcaatatt
     8941 caatattctc tgtgaatatg gaagaggatc aagtcttgtc catttttgaa ataaccgctt
     9001 atatatgcga ctgaatccaa ccctatgaat actacaatta tcattaacaa aggtgtaatt
     9061 tgagtatcct ggatttttga tacaaacaat acgatttgga aaattggtac gctgagcaac
     9121 ctgatacatc cacgtttcaa cagcagccgc tccgcgagga ggaattgaaa aaataggagt
     9181 aaccgtaaat attattttat caaccatctc gtatattaac ctttcataac attatattta
     9241 attgctgtaa aaacaccatc gagaaatttt ttctggtaaa gcaagtgttt atatttttct
     9301 ttgtgctcat gttttttaac agctttttta taaggtatat ttctccatgg tgatatatta
     9361 taaatattac gaaaataatc tgcagaggcg tagttcgccc aatcatgcca tggcttagtt
     9421 ataccagtat aatgtatgaa gacagtgtca tcattaataa accgggtgta atattcacta
     9481 tttttttctt caaattctga ctttatagta taaatacaat tatattttct cggtaatatt
     9541 ttagcatgat gtagaaacat taagtttaaa atatcttgat ccgggtactt taattttttt
     9601 ataattgact catctgataa tagatcaaag aatttttccg ttagtctttg ttttaaccat
     9661 tcccgtaaat taatatacat tacaccagag ttaaaatagc taccgttaaa atcctcatta
     9721 cacaaacgct cagcgctttt actttgcata gcatctacat caattaccac tgccccatat
     9781 tcatctttaa actctaatgc tattaactcg ttcaatgaac ccttacagac gatatcagca
     9841 tcaagatata ataatgaatc caatcgcgca gaaaaataat caaatgaaag caaacgataa
     9901 taaatagata ctggccagat atttgattga ggtagcgcct tcaatggctc agaatcaata
     9961 tgatataatt taattactgt ttgatacttc gttgctaatt gtgataataa ttcaatatac
    10021 tctttgtcta agtaatcagc gattatgtga aaggtaaatt tgttttgttt attcttttca
    10081 agaattgaag caattgaaat cgcagcccca acctggtagt tctcatcaat accccatgag
    10141 acattaaacg tttcccgttc atcaagttta acggggcgct tgtccagttc aattatatct
    10201 ttaaattgag taagatgttt aaaatccaat ttaattaccc ttcatcataa tcattatttg
    10261 ctcttcaatc ctagtttgac ttttacatcc ctgattactt ttttaatttt ctttttgaat
    10321 ataagatatg tatagccaaa gtctttcaat tcatttttta tattatagtg ccgttcaaga
    10381 tcaatgcgat cttttgcttt taaaacagcg gttgtttttt tacctgaaag gtcaattagt
    10441 cttaaacctt tttcggatac tataaaattt cctttatgag gatcgccaga caccattcca
    10501 tgctgatgca actcttttat cgattctgat aattgatcct tcagatcttc agaaatttcc
    10561 aaatactcat ttaacccgac accttcaata tactcaatga gcataatata ataatgagcg
    10621 aaatttaatg ttttacgttc agctagcaaa aaatagtcat taattgattg gatgccttca
    10681 ccacgtaccc gatcggtttg ataaattaaa ttttcataat aatctttttt tatgcaagat
    10741 tttaagaaac gttcggtcat tttatgcttt ggcgcataaa ctttaagtac aagaggtcca
    10801 cgagcagtgt caataagaat gactttagta tcatcaatag aacgaaaaac ttttaatgtt
    10861 ttaaccctgc aagttaaaaa gtcatttaga acttgctcat aaaagggatc attatctttt
    10921 atgaaaactt tcaacccatt tatcgtttta ttatatatca taatgaatta ttttaacctt
    10981 aaatctttag aagcattttt ctttataata ctttaaataa ttaaaaatgc ctgccatata
    11041 atgcttttgt ttaaatttat gttttgcaca ataccgatac tgattgctat ttacaggttt
    11101 aagtaaatct tctttactcc acggagaagc tgctttggcg atcaaaaaac tacgtgaaac
    11161 cggataatca gcccactcat gccagggttt tgtaggtccc acatagtgta taaacacggt
    11221 gtcatcattg actggattat caactttgtc ttttaattca tagttaatac tatatcgggt
    11281 attatatttc tccgaaataa acttcacttt accattcaat aatacattca gtacatcttg
    11341 atcaaggtgg gtgattttac ttacccaatc tgggtcacgc aacatttcaa tagcttttga
    11401 cgaaatgtta tttagattcc actcatcaat atttatcagt aaaaaaccag cattgaaata
    11461 tccagaagct aactgtggtg tagttaatac cgaggctcga ttttgccacc attcaatatc
    11521 tctttcagct acaactgcgg caatttcatt agtagaaaat tgataatcta agagttcttt
    11581 aatactaccc ttgcaagcaa tatctgcatc aagatatagt attttttcat gtttatgata
    11641 gaaataatct gcaattataa atcgaaaata tgttgcgtaa gtccagtttt tcgtgcttgg
    11701 taatgacttc agcttatcac aattgataac ataaatatta attcttgaat tatattgttt
    11761 tgctaaatca gaaaaatata atttgtcttt atcactgata taatctgtga aaatatgaaa
    11821 ttcacaagag atttctctat tgtttaatag aatagatgcg atggctacac cacatccaaa
    11881 aagaaaattt ctgtcaatac caaatgctat atcaagagta tttttagatc ttatattttg
    11941 ataatgaaaa ttatactcaa aaaggatgat gtcactatca ttgagttgag acatatttat
    12001 tctgtatcca aaattttggc aaaaataagt tacgggttac aacgattttc gaatcgttct
    12061 ttccctgatt tttgtggctt ttgcttctgc ttgcgacagc agtccttgtt cctgcttgag
    12121 aatgtcttta agcggggcgg caaaatacac cttcataaag cgccagatat cccgctgagt
    12181 caggccaata ttcattgaag aaaaataaag ccctataaga tctttatcgc gccaacgacc
    12241 tggaacgcgc gtgcgaagct gcgcccggtg taggtcaatt accgaaattt ttaactcctc
    12301 ttccttaccg gaaaaaggca agtgcagcag gaaatgacag atataacagt cacgatggtt
    12361 aatgcccgca gcatgcatat cgcgcaccat cgtcgcgaca cgcttaataa gcatacgctt
    12421 tacgcgaaca tctggtgggt tggtcgccca gtcagcacag taatcttcca gactgatggt
    12481 tggtgtcaga tcttcggtaa taataaacga ggttctggtc agcggattca tgcctttttc
    12541 gccgaatgcc accccataca tagtatcaac gccgacatcc cgcagtcgat gaatcgcatt
    12601 ccattcgcgg tctgcgccta atactggcat ccgcaatgag agtaaatttt tgattatctc
    12661 tttcagggtc gtgccgcgat gccatttgag aaaatagctt tttcccgcca tttcaaagcg
    12721 cagagtacgg cgagtttcca gttcacgaaa tacctcaccc tgcaaggttt taacttcctc
    12781 aaaaggatct ttgccgcgcc ataacgtggc aaacggctct ttaagttcaa ccatccagac
    12841 cacccgttat gatatccgcc gctttctctg gcagactgta taaatcttgt gtatcagcat
    12901 aatgtcgcgc attttccgcc caagcctggc gcaatgaaga ttgcgttaac gctttgcgta
    12961 aaatctcatt caatgtttcc tggcggaatg gctcagcaat agcctcgccg caattagcgt
    13021 cgacaatata atgcgcatag ccacaaacgg cagttgttag taccggtaat cctgcagtaa
    13081 tcgcttccag cagcacaatt cccgccgctt cctggtaggc aggatgcagt aataaatccg
    13141 ccgccgccat taattccgag acatcgttgc gccccgagaa gaagtgaaca ttactgcgca
    13201 cgccgcgttt ttctgccagt gcctcaaatt ttcgcggttt atcctgccca acaacatata
    13261 gcaatgtgtt gtggcgcagc gaatccggta acgaagcaag tgcttcaatg gaacgatcgg
    13321 cacctttacg cgtgaagtct gaaccgacct gcaacaataa atattgttgt tcggttattc
    13381 cattcttctt acggaagatt tcacggctat tggctggctg ctggctatat ttacgatcag
    13441 gataaatccc cggtggcaga atatgaaaac gctccgcttc agtctgataa tgtttctgga
    13501 aatcggcgat ttgcttatct gtcagcatca gcagctgtgt cggcttgccc tgttcgaagg
    13561 ttgcccgctc aaaggcggca taatggcgat aacgtgacgt caggcgatag aaaaagcctt
    13621 tttcctgcgc tactttctcg gcataacaaa catcagcggc ataataaacg tccagccccg
    13681 gcattttgtt gaatccaacg actttatcga cgggatgttc gcgtaaatgt ttttgcaccc
    13741 aggcaaaata ctccgcattg cgcccgtgat tggtatgcga tttaaccggc actttgatca
    13801 cttcaaatac atcagggcat tcgccttccc acgactgggt ataaacccga acatgatgac
    13861 ctcgggcggc gactgtctga gcaatacgca taaaatcgcg ctgcaaaccg ccaaagggaa
    13921 aatatttata taaacaaaaa gcaacgatca taacgacgta cctgtcgtgg aggagggcag
    13981 taatttatcg acagcagcaa tgacatctgc cgccggaata accgaaagat acatctcatt
    14041 tcggtcacgc tgatcgcgcg ttggcatttc ccggtaatct cccgcccaga attgaatcat
    14101 gttatttgac cagggacgcc agaaaatatg atctgttgcg ccaaacagcg atatcagcgg
    14161 cgtattaact gcagcggcaa tatgcgccgg tgcggaatca acgccaataa acagctgcgc
    14221 atgatcgatt aacgcaccaa gttccgggaa ggtcaccttt ccagccagcg ccgttactgg
    14281 tggcgtctgg catccctgcg caatttcatt gacgcaggcc agatcgtctt tatctgggcc
    14341 ggacgtcaga acgacttcat aaccacgagc atgtaaggca tcaatcacag cggaaaactt
    14401 ggcgttgtcc cagcatttga agatttgccg cgccgtaggt tggataacca cataattttg
    14461 tccaacacca gcgtgatcaa gttcgcgacg catacgtttc cagcttgcag gcgggtaact
    14521 catggttgtc tgcttcacca acgaatcaag tcccaatggt gtcagcacgg ataagttact
    14581 ttccaccaca tttccaccct gcaacggcac caaatgggtg aaacttttac gccaaaaagc
    14641 agactgccga tgatgataat cctgggaaat tttcacacga gcatttaata agcgaaccag
    14701 tatagcaacc atccattgat cggtaagatt gacgataagg tcatacttat tggcacgtaa
    14761 taccttgatg agatgaaaaa agttggcaat tttttctgag gcttttgctt ttttattttt
    14821 tatgccgtag agcgcgttaa tctctggatt ttcagacagg atcgggatgg tgtcctgata
    14881 aagcagcaca tcgatttttg cgtcagggta attttttttc agagaactaa tgacgggagt
    14941 agttaataac atatccccat gaaaacgcat cttaatgagc aaaattcttc gaaatggctt
    15001 atccacaagt aactcttttt gttgagatta tccgattaag gtgaagcaga aaaagcacgc
    15061 taccgcccca ggctcaacag ctacctgaat actgataccg cactgatatt gacgcgctag
    15121 tgtatcactt ctttcgcggc aatccgaccc gaatgatttt taagcacatt gttgattttc
    15181 aataaagaaa tcaatcaaat aaaatcatta gcaaccatat tctttcactg ttgctctgcg
    15241 attccttcct gcacgtacag cagaaatcaa tgaatcgcat aaaaatacaa aaaaagtata
    15301 gatgttcaac aagaattggc gggtaaattg ctatttaagt caaa
//
LOCUS       DAJTRC010000087        15344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.087, whole genome shotgun sequence.
ACCESSION   DAJTRC010000087 DAJTRC010000000
VERSION     DAJTRC010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 15344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.087"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            201..395
                     /gene="bfd"
                     /locus_tag="OO357_003027"
     CDS             201..395
                     /gene="bfd"
                     /locus_tag="OO357_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin-associated ferredoxin"
                     /protein_id="HCR5887892.1"
                     /translation="MYVCLCNGVSDKKIRQAVRQFHPQSFQQLRKFIPVGNQCGKCIR
                     AAREVMQDELMQMPEFKEIA"
     gene            468..944
                     /gene="bfr"
                     /locus_tag="OO357_003028"
     CDS             468..944
                     /gene="bfr"
                     /locus_tag="OO357_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HCR5887893.1"
                     /translation="MKGDVKIINYLNKLLGNELVAINQYFLHARMFKNWGLMRLNDVE
                     YHESIDEMKHADKYIERILFLEGIPNLQDLGKLGIGEDVEEMLQSDLRLELEGAKDLR
                     EAIAYADSVHDYVSRDMMIEILADEEGHIDWLETELDLIGKIGLQNYLQSQIKVKD"
     gene            complement(941..1408)
                     /locus_tag="OO357_003029"
                     /pseudo
     CDS             complement(941..1408)
                     /locus_tag="OO357_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312215.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
     gene            1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO357_003030"
     CDS             1787..2098
                     /gene="rpsJ"
                     /locus_tag="OO357_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HCR5887894.1"
                     /translation="MQNQRIRIRLKAFDHRLIDQATAEIVETAKRTGAQVRGPIPLPT
                     RKERFTVLISPHVNKDARDQYEIRTHLRLVDIVEPTEKTVDALMRLDLAAGVDVQISL
                     G"
     gene            2131..2760
                     /gene="rplC"
                     /locus_tag="OO357_003031"
     CDS             2131..2760
                     /gene="rplC"
                     /locus_tag="OO357_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000579825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HCR5887895.1"
                     /translation="MIGLVGKKVGMTRIFTEDGVSIPVTVIEVEANRVTQVKDLANDG
                     YRAIQVTTGAKKANRVTKPEAGHFAKAGVEAGRGLWEFRLAEGEEFTVGQSISVELFA
                     DVKKVDVTGTSKGKGFAGTVKRWNFRTQDATHGNSLSHRVPGSIGQNQTPGKVFKGKK
                     MAGQMGNERVTVQSLDVVRVDAERNLLLVKGAVPGATGSDLIVKPAVKA"
     gene            2771..3376
                     /gene="rplD"
                     /locus_tag="OO357_003032"
     CDS             2771..3376
                     /gene="rplD"
                     /locus_tag="OO357_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010304939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HCR5887896.1"
                     /translation="MELVLKDAQSALTVSETTFGRDFNEALVHQVVVAYAAGARQGTR
                     AQKTRAEVTGSGKKPWRQKGTGRARSGSIKSPIWRSGGVTFAARPQDHSQKVNKKMYR
                     GALKSILSELVRQDRLIVVEKFSVEAPKTKLLAQKLKDMALEDVLIITGELDENLFLA
                     ARNLQKVDVRDATGIDPVSLIAFDKVVMTADAVKQVEEMLA"
     gene            3373..3675
                     /gene="rplW"
                     /locus_tag="OO357_003033"
     CDS             3373..3675
                     /gene="rplW"
                     /locus_tag="OO357_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007891652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HCR5887897.1"
                     /translation="MIREERLLKVLRAPHVSEKASTAMEKSNTIVLKVAKDATKAEIK
                     AAVQKLFEVEVEVVNTLVVKGKVKRHGQRIGRRSDWKKAYVTLKEGQNLDFVGGAE"
     gene            3693..4514
                     /gene="rplB"
                     /locus_tag="OO357_003034"
     CDS             3693..4514
                     /gene="rplB"
                     /locus_tag="OO357_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001738551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HCR5887898.1"
                     /translation="MAVVKCKPTSPGRRHVVKVVNPELHKGKPFAPLLEKNSKSGGRN
                     NNGRITTRHIGGGHKQAYRIVDFKRNKDGIPAVVERLEYDPNRSANIALVLYKDGERR
                     YILAPKGLKAGDQIQSGVDAAIKPGNTLPMRNIPVGSTVHNVEMKPGKGGQLARSAGT
                     YVQIVARDGAYVTLRLRSGEMRKVEADCRATLGEVGNAEHMLRVLGKAGAARWRGVRP
                     TVRGTAMNPVDHPHGGGEGRNFGKHPVTPWGVQTKGKKTRSNKRTDKFIVRRRSK"
     gene            4531..4809
                     /gene="rpsS"
                     /locus_tag="OO357_003035"
     CDS             4531..4809
                     /gene="rpsS"
                     /locus_tag="OO357_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HCR5887899.1"
                     /translation="MPRSLKKGPFIDLHLLKKVEKAVESGDKKPLRTWSRRSTIFPNM
                     IGLTIAVHNGRQHVPVFVTDEMVGHKLGEFAPTRTYRGHAADKKAKKK"
     gene            4824..5156
                     /gene="rplV"
                     /locus_tag="OO357_003036"
     CDS             4824..5156
                     /gene="rplV"
                     /locus_tag="OO357_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000447526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HCR5887900.1"
                     /translation="METIAKHRHARSSAQKVRLVADLIRGKKVSQALDILTYTNKKAA
                     VQVKKVLESAIANAEHNDGADIDDLKVTKIFVDEGPSMKRIMPRAKGRADRILKRTSH
                     ITVVVSDR"
     gene            5174..5875
                     /gene="rpsC"
                     /locus_tag="OO357_003037"
     CDS             5174..5875
                     /gene="rpsC"
                     /locus_tag="OO357_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001742302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HCR5887901.1"
                     /translation="MGQKVHPNGIRLGIVKPWNSTWFANTKEFADNLDSDFKVRQYLT
                     KELAKASVSRIVIERPAKSIRVTIHTARPGIVIGKKGEDVEKLRKVVADIAGVPAQIN
                     IAEVRKPELDAKLVADSITSQLERRVMFRRAMKRAVQNAMRLGAKGIKVEVSGRLGGA
                     EIARTEWYREGRVPLHTLRADIDYNTSEAHTTYGVIGVKVWIFKGEILGGMAAVEQPE
                     KPAAQPKKQQRKGRK"
     gene            5888..6298
                     /gene="rplP"
                     /locus_tag="OO357_003038"
     CDS             5888..6298
                     /gene="rplP"
                     /locus_tag="OO357_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017805229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HCR5887902.1"
                     /translation="MLQPKRTKFRKMHKGRNRGLAQGTDVSFGSFGLKAVGRGRLTAR
                     QIEAARRAMTRAVKRQGKIWIRVFPDKPITEKPLAVRMGKGKGNVEYWVALIQPGKVL
                     YEMDGVPEELAREAFKLAAAKLPIKTTFVTKTVM"
     gene            6298..6489
                     /gene="rpmC"
                     /locus_tag="OO357_003039"
     CDS             6298..6489
                     /gene="rpmC"
                     /locus_tag="OO357_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000644743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HCR5887903.1"
                     /translation="MKAKELREKSIEELNTELLNLLREQFNLRMQAASGQLQQSHLLK
                     QVRRDVARVKTLLNEKAGA"
     gene            6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO357_003040"
     CDS             6489..6743
                     /gene="rpsQ"
                     /locus_tag="OO357_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006817275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HCR5887904.1"
                     /translation="MTDKIRTLQGRVVSDKMEKSIVVAIERFVKHPIYGKFIKRTTKL
                     HVHDENNECGIGDVVEIRECRPLSKTKSWTLVRVVEKAVL"
     gene            6908..7279
                     /gene="rplN"
                     /locus_tag="OO357_003041"
     CDS             6908..7279
                     /gene="rplN"
                     /locus_tag="OO357_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015817037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HCR5887905.1"
                     /translation="MIQEQTMLNVADNSGARRVMCIKVLGGSHRRYAGVGDIIKITIK
                     EAIPRGKVKKGDVLKAVVVRTKKGVRRPDGSVIRFDGNACVLLNNNSEQPIGTRIFGP
                     VTRELRSEKFMKIISLAPEVL"
     gene            7290..7604
                     /gene="rplX"
                     /locus_tag="OO357_003042"
     CDS             7290..7604
                     /gene="rplX"
                     /locus_tag="OO357_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000729185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HCR5887906.1"
                     /translation="MAAKIRRDDEVIVLTGKDKGKRGKVKNVLSSGKVIIEGINLVKK
                     HQKPVPALNQPGGIVEKEAAIQVSNVAIFNAATGKADRVGFRFEDGKKVRFFKSNSET
                     IK"
     gene            7619..8158
                     /gene="rplE"
                     /locus_tag="OO357_003043"
     CDS             7619..8158
                     /gene="rplE"
                     /locus_tag="OO357_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001096198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HCR5887907.1"
                     /translation="MAKLHDYYKDEVVKKLMTEFNYNSVMQVPRVEKITLNMGVGEAI
                     ADKKLLDNAAADLAAISGQKPLITKARKSVAGFKIRQGYPIGCKVTLRGERMWEFFER
                     LITIAVPRIRDFRGLSAKSFDGRGNYSMGVREQIIFPEIDYDKVDRVRGLDITITTTA
                     KSDEEGRALLAAFDFPFRK"
     gene            8173..8478
                     /gene="rpsN"
                     /locus_tag="OO357_003044"
     CDS             8173..8478
                     /gene="rpsN"
                     /locus_tag="OO357_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005599304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HCR5887908.1"
                     /translation="MAKQSMKAREVKRVALADKYFAKRAELKAIISDVNASDEDRWNA
                     VLKLQTLPRDSSPSRQRNRCRQTGRPHGFLRKFGLSRIKVREAAMRGEIPGLKKASW"
     gene            8512..8904
                     /gene="rpsH"
                     /locus_tag="OO357_003045"
     CDS             8512..8904
                     /gene="rpsH"
                     /locus_tag="OO357_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011148781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HCR5887909.1"
                     /translation="MSMQDPIADMLTRIRNGQAANKAAVTMPSSKLKVAIANVLKEEG
                     FIEDFKVEGDTKPELELTLKYFQGKAVVESIQRVSRPGLRIYKRKDELPKVMAGLGIA
                     VVSTSKGVMTDRAARQAGLGGEIICYVA"
     gene            8917..9450
                     /gene="rplF"
                     /locus_tag="OO357_003046"
     CDS             8917..9450
                     /gene="rplF"
                     /locus_tag="OO357_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HCR5887910.1"
                     /translation="MSRVAKAPVVVPAGVDVKINGQVITIKGKNGELTRTLNDAVEVK
                     HADNTLTFGPRDGYADGWAQAGTARALLNSMVIGVTEGFTKKLQLVGVGYRAAVKGNV
                     INLSLGFSHPVDHQLPAGITAECPTQTEIVLKGADKQVIGQVAADLRAYRRPEPYKGK
                     GVRYADEVVRTKEAKKK"
     gene            9460..9813
                     /gene="rplR"
                     /locus_tag="OO357_003047"
     CDS             9460..9813
                     /gene="rplR"
                     /locus_tag="OO357_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HCR5887911.1"
                     /translation="MDKKSARIRRATRARRKLQELGATRLVVHRTPRHIYAQVIAPNG
                     SEVLVAASTVEKAIAEQLKYTGNKDAAAAVGKAVAERALEKGIKDVSFDRSGFQYHGR
                     VQALADAAREAGLQF"
     gene            9828..10331
                     /gene="rpsE"
                     /locus_tag="OO357_003048"
     CDS             9828..10331
                     /gene="rpsE"
                     /locus_tag="OO357_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004106339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HCR5887912.1"
                     /translation="MAHIEKQAGELQEKLIAVNRVSKTVKGGRIFSFTALTVVGDGNG
                     RVGFGYGKAREVPAAIQKAMEKARRNMINVALNNGTLQHPVKGVHTGSRVFMQPASEG
                     TGIIAGGAMRAVLEVAGVHNVLAKAYGSTNPINVVRATIDGLENMNSPEMVAAKRGKS
                     VEEILGK"
     gene            10335..10514
                     /gene="rpmD"
                     /locus_tag="OO357_003049"
     CDS             10335..10514
                     /gene="rpmD"
                     /locus_tag="OO357_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012147215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HCR5887913.1"
                     /translation="MAKTIKITQTRSAIGRLPKHKATLLGLGLRRIGHTVEREDTPAI
                     RGMINAVSFMVKVEE"
     gene            10518..10952
                     /gene="rplO"
                     /locus_tag="OO357_003050"
     CDS             10518..10952
                     /gene="rplO"
                     /locus_tag="OO357_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011095501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HCR5887914.1"
                     /translation="MRLNTLSPAEGSKKAGKRLGRGIGSGLGKTGGRGHKGQKSRSGG
                     GVRRGFEGGQMPLYRRLPKFGFTSRKAAITAEVRLSDLAKVEGGVVDLNTLKAANIIG
                     IQIEFAKVILAGEVTTPVTVRGLRVTKGARAAIEAAGGKIEE"
     gene            10960..12291
                     /gene="secY"
                     /locus_tag="OO357_003051"
     CDS             10960..12291
                     /gene="secY"
                     /locus_tag="OO357_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004868351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HCR5887915.1"
                     /translation="MAKQPGLDFQSAKGGLGELKRRLLFVIGALIVFRIGSFIPIPGI
                     DAAVLAKLLEQQRGTIIEMFNMFSGGALSRASIFALGIMPYISASIIIQLLTVVHPTL
                     AEIKKEGESGRRKISQYTRYGTLVLAIFQSIGIATGLPNMPGMQGLVINPGFAFYFTA
                     VVSLVTGTMFLMWLGEQITERGIGNGISIIIFAGIVAGLPPAIAHTIEQARQGDLHFL
                     VLLLVAVLVFAVTFFVVFVERGQRRIVVNYAKRQQGRRVYAAQSTHLPLKVNMAGVIP
                     AIFASSIILFPATIASWFGGGTGWNWLTTISLYLQPGQPLYVLLYASAIIFFCFFYTA
                     LVFNPRETADNLKKSGAFVPGIRPGEQTAKYIDKVMTRLTLVGALYITFICLIPEFMR
                     DAMKVPFYFGGTSLLIVVVVIMDFMAQVQTLMMSSQYESALKKANLKGYGR"
     gene            12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO357_003052"
     CDS             12323..12439
                     /gene="rpmJ"
                     /locus_tag="OO357_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020322760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HCR5887916.1"
                     /translation="MKVRASVKKLCRNCKIVKRDGVIRVICSAEPKHKQRQG"
     gene            12586..12942
                     /gene="rpsM"
                     /locus_tag="OO357_003053"
     CDS             12586..12942
                     /gene="rpsM"
                     /locus_tag="OO357_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019935869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HCR5887917.1"
                     /translation="MARIAGINIPDHKHAVIALTSIYGVGKTRSKAILAAAGIAEDVK
                     ISELSEGQIDTLRDEVAKFVVEGDLRREISMSIKRLMDLGCYRGLRHRRGLPVRGQRT
                     KTNARTRKGPRKPIKK"
     gene            12959..13348
                     /gene="rpsK"
                     /locus_tag="OO357_003054"
     CDS             12959..13348
                     /gene="rpsK"
                     /locus_tag="OO357_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HCR5887918.1"
                     /translation="MAKAPIRARKRVRKQVSDGVAHIHASFNNTIVTITDRQGNALGW
                     ATAGGSGFRGSRKSTPFAAQVAAERCADAVKEYGIKNLEVMVKGPGPGRESTIRALNA
                     AGFRITNITDVTPIPHNGCRPPKKRRV"
     gene            13382..14002
                     /gene="rpsD"
                     /locus_tag="OO357_003055"
     CDS             13382..14002
                     /gene="rpsD"
                     /locus_tag="OO357_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HCR5887919.1"
                     /translation="MARYLGPKLKLSRREGTDLFLKSGVRAIDTKCKIEQAPGQHGAR
                     KPRLSDYGVQLREKQKVRRIYGVLERQFRNYYKEAARLKGNTGENLLALLEGRLDNVV
                     YRMGFGATRAEARQLVSHKAIMVNGRVVNIASYQVSPNDVVSIREKAKKQSRVKAALE
                     LAEQREKPTWLEVDAGKMEGTFKRKPERSDLSADINEHLIVELYSK"
     gene            14028..15017
                     /gene="rpoA"
                     /locus_tag="OO357_003056"
     CDS             14028..15017
                     /gene="rpoA"
                     /locus_tag="OO357_003056"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005970247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HCR5887920.1"
                     /translation="MQGSVTEFLKPRLVDIEQVSSTHAKVTLEPLERGFGHTLGNALR
                     RILLSSMPGCAVTEVEIDGVLHEYSTKEGVQEDILEILLNLKGLAVRVQGKDEVILTL
                     NKSGIGPVTAADITHDGDVEIVKPQHVICHLTDENASISMRIKVQRGRGYVPASTRIH
                     SEEDERPIGRLLVDACYSPVERIAYNVEAARVEQRTDLDKLVIEMETNGTIDPEEAIR
                     RAATILAEQLEAFVDLRDVRQPEVKEEKPEFDPILLRPVDDLELTVRSANCLKAEAIH
                     YIGDLVQRTEVELLKTPNLGKKSLTEIKDVLASRGLSLGMRLENWPPASIADE"
     gene            15058..>15344
                     /gene="rplQ"
                     /locus_tag="OO357_003057"
     CDS             15058..>15344
                     /gene="rplQ"
                     /locus_tag="OO357_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HCR5887921.1"
                     /translation="MRHRKSGRQLNRNSSHRQAMFRNMAGSLVRHEIIKTTLPKAKEL
                     RRVVEPLITLAKTDSVANRRLAFARTRDNEIVAKLFNELGPRFASRAGGYTR"
BASE COUNT         3961 a         3599 c         4087 g         3697 t
ORIGIN      
        1 gctaaagttc tgggctaatt acacgttaat tagttttgaa ttgaaaaggg cgcttcggcg
       61 ccctttttgc tgcccgtaat ttgagctcga aatatttagt cgtaattttg ctcgctgaac
      121 tatggcgcaa ataaaactgt gctattgtaa tcataaccat tctcatttac actttgtgca
      181 gaatttgaac gggagcaatc atgtacgttt gtttgtgtaa tggtgtaagt gacaaaaaaa
      241 ttcgccaggc agtccgtcag tttcatccgc aatctttcca acaattgcgt aagtttattc
      301 ctgtgggaaa tcaatgcggt aagtgcattc gcgccgcgcg cgaagtgatg caggacgagt
      361 tgatgcagat gccagaattt aaagagattg cctgaagccc acgcttattt tttgacatcc
      421 tgctagcccc atctacgctc taatagtgga agcggaggga ctataaaatg aaaggtgatg
      481 ttaaaatcat aaattatctc aataaactat tgggaaatga gcttgtcgca atcaatcagt
      541 attttctcca tgcccggatg tttaaaaact ggggcctgat gcgtctcaat gacgtcgaat
      601 accatgaatc catcgatgag atgaaacacg ctgataaata tatcgagcgt attttgtttt
      661 tagaaggaat ccccaactta caggatttag gcaaactggg gattggtgaa gatgtcgagg
      721 agatgctgca atctgacctg cgattagaac tggaaggcgc gaaggatctg cgtgaggcta
      781 tcgcatatgc cgacagcgtt catgactatg ttagccgcga tatgatgatc gagatccttg
      841 ctgatgaaga aggtcatatc gactggctcg aaactgaact ggatctcatc ggtaaaatcg
      901 gcctacaaaa ttacctgcaa tcacaaatta aagttaaaga ttaacggcct atcagcaaac
      961 tatcccagcc tataacgaaa cccgcagccg ccagaaatgg cccaaaaggc agcgggtttt
     1021 ttaatgattc ttttcctctc atgagcagac caataactac ggctccgcaa gcgaatgagg
     1081 cagcaaggaa aaccagtcgt ggtaagaagg cccatgaatg ctaggcgcca agggcggcga
     1141 gaaacttcac atcaccatag cctaatcctt ctttatgtcg cagtatgcga tacccccagt
     1201 aaatgacagc gaatgtgccg taaccaacaa ttgcccccca taacgcatcg gctaaaccat
     1261 caggatgaca aacttgatag aacaaaagac ctgaccagag taacgggcac gtaaatctgt
     1321 caggtaacaa gccatgcttt gcatcccaga aaaaaagtag ggctgataaa caagcgtaca
     1381 aaattagaaa gggaagggta gtgtccataa aacatcctct gtaattggag tatcaccatc
     1441 gaataactcc ggatgctcag caacagagag agcgctgatt tgttaacctg attccggtga
     1501 aaaacgctat cttcacaacc ttacaaattt cgtggaatgc agctcgcaat ctgcgcgttg
     1561 cgattaattt ctacccgatg cttgcgtcag gctcagattt gcgtataatg cgcgggcttg
     1621 tcgtagttga cagcaggttc aatctaaacc tcagttagcc gaatttggct acctaacaat
     1681 gctcccaatc ggggagctac gtaagaacgg ttacactctc ccatcaatcg taatgggtct
     1741 gaggagtaat cattttcgtt tataaaataa ttggagctct ggtctcatgc agaaccaaag
     1801 aatccgtatc cgcctgaaag cgtttgatca tcgtctgatc gatcaagcaa ccgcggaaat
     1861 cgtcgagact gccaagcgca ctggtgcgca ggtccgtggt ccgatcccgc tgccgacacg
     1921 caaagagcgc ttcactgttc tgatctcccc gcacgtcaac aaagacgcgc gcgatcagta
     1981 cgaaatccgt actcacttgc gtctggttga catcgttgag ccaaccgaga aaaccgttga
     2041 tgctctgatg cgtctggatc tggctgccgg tgtagacgtg cagatcagcc tgggttaatc
     2101 aggtcattga gcgattgaga ggttgaaaca atgattggtt tagtcggtaa aaaagtgggt
     2161 atgacccgta tcttcacaga agacggcgtt tctatcccag taaccgtaat cgaagttgaa
     2221 gcaaaccgcg ttactcaggt taaagacctg gctaacgatg gctaccgtgc tattcaggtg
     2281 accaccggtg ctaaaaaagc taaccgtgtg accaagcctg aagctggcca cttcgctaaa
     2341 gctggcgtag aagctggccg tggtctgtgg gaattccgcc tggctgaagg cgaagagttc
     2401 actgtaggtc agagcattag cgttgaactg tttgctgacg ttaaaaaagt tgacgtaact
     2461 ggcacctcta aaggtaaagg tttcgcaggt accgttaagc gctggaactt ccgtacccag
     2521 gacgctactc acggtaactc cttgtctcac cgcgttccgg gttctatcgg tcagaaccag
     2581 actccgggca aagtgttcaa aggcaagaaa atggcaggtc agatgggtaa cgaacgtgta
     2641 accgttcaga gccttgacgt agtacgcgtt gacgctgagc gcaacctgct gctggttaaa
     2701 ggtgctgtcc cgggtgcaac cggtagcgac ctgatcgtta aaccagctgt gaaggcgtaa
     2761 ggagatagca atggaattag tattgaaaga cgcgcagagc gcgctgactg tttccgaaac
     2821 taccttcggt cgtgatttca acgaagcgct ggttcaccag gttgttgttg cttatgcagc
     2881 tggtgctcgt cagggtactc gtgctcagaa gactcgtgct gaagtaactg gttccggtaa
     2941 aaaaccgtgg cgccagaaag gcaccggccg tgcgcgttct ggttctatca agagcccgat
     3001 ctggcgttct ggtggcgtga cctttgctgc tcgtccgcag gaccacagtc aaaaagttaa
     3061 caagaagatg taccgcggcg cgctgaaaag catcctgtcc gaactggtac gtcaggatcg
     3121 tctgatcgtt gtcgagaagt tctctgtaga agcgccgaaa actaagctgc tggcacagaa
     3181 actgaaagac atggctctgg aagatgtgct gatcatcacc ggtgagctgg acgaaaacct
     3241 gttcctggct gcgcgcaacc tgcagaaggt tgacgtacgc gatgcaactg gtatcgaccc
     3301 ggttagcctg atcgccttcg acaaagtcgt aatgactgct gatgctgtta agcaagttga
     3361 ggagatgctg gcatgattcg tgaagaacgt ctgctgaagg tgctgcgtgc accgcacgtt
     3421 tctgaaaaag cgtctactgc gatggaaaaa tccaacacca tcgtactcaa agttgctaaa
     3481 gacgcgacca aagcagaaat caaagctgct gtgcagaaac tgtttgaagt cgaagtcgaa
     3541 gtcgttaaca ccctggtagt taaagggaaa gttaaacgtc acggacagcg tatcggtcgt
     3601 cgtagcgact ggaaaaaagc ttacgtcacc ctgaaagaag gccagaatct ggacttcgtt
     3661 ggcggcgctg agtaagtcgg aggagtaata caatggcagt tgttaaatgt aaaccgacat
     3721 ctccgggtcg tcgccacgta gttaaagtgg ttaaccctga gctgcacaag ggcaaacctt
     3781 ttgctccgtt gctggaaaaa aacagcaaat ccggtggtcg taacaacaat ggccgtatca
     3841 ccactcgtca tatcggtggt ggccacaagc aggcttaccg tattgttgac ttcaaacgca
     3901 acaaagacgg tatcccggca gttgttgaac gtcttgagta cgatccgaac cgttccgcga
     3961 acatcgcgct ggttctgtac aaagacggtg aacgccgtta catcctggcc cctaaaggcc
     4021 tgaaagctgg cgaccagatt cagtctggcg ttgatgctgc aatcaaacca ggtaacaccc
     4081 tgccgatgcg caacatcccg gttggttcta ctgttcataa cgtagaaatg aaaccaggta
     4141 aaggcggtca gctggcacgt tccgctggta cttacgttca gatcgttgct cgtgatggtg
     4201 cttatgtcac cctgcgtctg cgttctggtg aaatgcgtaa agtagaagca gactgccgtg
     4261 caactctggg cgaagttggc aatgctgagc atatgctgcg cgttctgggt aaagcaggtg
     4321 ctgcacgctg gcgtggtgtt cgtccgaccg ttcgcggtac cgcgatgaac ccggtagacc
     4381 acccacatgg tggtggtgaa ggtcgtaact ttggtaagca cccggtaact ccgtggggcg
     4441 ttcagaccaa aggtaagaag acccgcagca acaagcgtac tgataaattc atcgtacgtc
     4501 gccgtagcaa ataattttag aggataagcc atgccacgtt ctctcaagaa aggtcctttt
     4561 attgacctgc acttgctgaa gaaggtagag aaagcggtgg aaagcggaga caagaagccc
     4621 ctgcgcactt ggtcccgtcg ttcaacgatc tttcctaaca tgatcggttt gaccatcgct
     4681 gtccataatg gtcgtcagca cgttccggta tttgtaaccg acgaaatggt cggtcacaaa
     4741 ctgggtgaat tcgcaccgac tcgtacttat cgcggccacg ctgctgataa aaaagcgaag
     4801 aagaaataag gtaggaggaa gagatggaaa ctattgctaa acatcgccat gctcgttctt
     4861 ctgctcagaa ggttcgcctt gttgctgacc tgattcgcgg taagaaagtg tcgcaggctc
     4921 tggatatttt gacctacacc aacaagaaag cggctgtaca ggtcaagaaa gttctggaat
     4981 ctgccattgc taacgctgaa cacaacgatg gcgctgacat tgacgatctg aaagttacga
     5041 aaattttcgt agacgaaggc ccgagcatga agcgcattat gccgcgtgca aaaggtcgtg
     5101 cagatcgcat cctgaagcgc accagccaca tcactgtggt tgtgtccgat cgctgagact
     5161 ctggagacta gcaatgggtc agaaagtaca tcctaatggt attcgcttgg gtattgtaaa
     5221 accatggaac tctacttggt ttgcgaacac caaagaattc gctgacaacc tggacagcga
     5281 ttttaaagta cgtcagtacc tgactaagga actggctaaa gcgtccgtat ctcgtatcgt
     5341 tatcgagcgt ccggctaaga gcatccgtgt aaccattcac actgctcgcc cgggtatcgt
     5401 tatcggtaaa aaaggtgaag acgtagaaaa actgcgtaag gtcgtagcgg acatcgctgg
     5461 cgttcctgca cagatcaaca tcgccgaagt tcgtaagcct gaactggacg caaaactggt
     5521 tgctgacagc atcacttctc agctggaacg tcgcgttatg ttccgtcgtg ctatgaagcg
     5581 tgctgtacag aacgcaatgc gtctgggcgc taaaggtatt aaagttgaag ttagcggccg
     5641 tctgggcggc gcggaaatcg cacgtaccga atggtaccgc gaaggtcgcg taccgctgca
     5701 cactctgcgt gctgacatcg actacaacac ctctgaagcg cacaccactt acggtgtaat
     5761 cggcgttaaa gtgtggatct tcaaaggcga gatcctgggt ggtatggctg ctgttgaaca
     5821 accggaaaaa ccggctgctc agcctaaaaa gcagcagcgt aaaggccgta aataaggagc
     5881 gtcgctgatg ttacaaccaa agcgtacaaa attccgtaaa atgcacaaag gccgtaaccg
     5941 cggtctggcg cagggtacgg atgttagctt cggcagcttc ggtctgaaag ctgttggccg
     6001 tggtcgtctg actgcccgtc agatcgaagc agcacgtcgt gctatgaccc gtgcagttaa
     6061 gcgtcaaggt aagatctgga tccgtgtgtt cccggacaaa ccgatcactg aaaagccgct
     6121 ggcagtgcgt atgggtaaag gtaaaggtaa cgtggagtat tgggttgcct tgattcagcc
     6181 gggtaaagtc ctgtatgaaa tggacggtgt tccggaagag ctggcccgtg aagcattcaa
     6241 gctggcagca gcgaaactgc cgattaaaac cacctttgta actaagacgg tgatgtaatg
     6301 aaagcaaaag agctgcgtga gaagagcatt gaagagctga acaccgagct gctgaacctg
     6361 ctgcgtgagc agttcaacct gcgtatgcag gctgcaagtg gccagctgca acagtctcac
     6421 ctgttgaagc aagtgcgtcg cgatgtcgca cgcgttaaga ctttactgaa cgagaaggcg
     6481 ggtgcgtaat gaccgataaa atccgtactc tgcaaggtcg cgttgttagc gacaaaatgg
     6541 agaaatccat tgttgttgct atcgaacgtt ttgtgaaaca cccgatctac ggaaaattca
     6601 tcaagcgtac gaccaaactg cacgtacatg acgagaacaa cgaatgcggt atcggtgacg
     6661 tggttgaaat ccgcgaatgc cgtccgctgt ccaagactaa atcctggacg ctggttcgcg
     6721 ttgtagagaa agcggttctg taatacagta cactctctcg atacgaataa acggctcaga
     6781 aatgagccgt ttattttttc tacccatatc cttgaagcgg tgttataatg ccgcgccctc
     6841 gatatgggga tttttaacga cctgattttc gggtctcagt agtagttgac attagcggag
     6901 cactaaaatg atccaagaac agactatgct gaacgtcgcc gacaactccg gtgcacgtcg
     6961 cgtaatgtgt atcaaggttc tgggtggctc gcaccgtcgc tacgcaggcg taggcgacat
     7021 catcaagatc accatcaaag aagcaattcc gcgtggtaag gtcaaaaaag gtgatgtgct
     7081 gaaggcggta gtggtgcgca ccaagaaggg tgttcgtcgc ccggacggtt ctgtcattcg
     7141 cttcgatggt aatgcttgtg ttcttctgaa caacaacagc gagcagccta tcggtacgcg
     7201 tatttttggg ccggtaactc gtgagcttcg tagtgagaag ttcatgaaaa ttatctctct
     7261 ggcaccagaa gtactctaag gagcgaatca tggcagcgaa aatccgtcgt gatgacgaag
     7321 ttatcgtgtt aaccggtaaa gataaaggta aacgcggtaa agttaagaat gtcctgtctt
     7381 ccggcaaggt cattattgaa ggtatcaacc tggttaagaa acatcagaag ccggttccgg
     7441 ccctgaacca accgggtggc atcgttgaaa aagaagccgc tattcaggtt tccaacgtag
     7501 caatcttcaa tgcggcaacc ggcaaggctg accgtgtagg ctttagattc gaagacggca
     7561 aaaaagtccg tttcttcaag tctaacagcg aaactatcaa gtaatttgga gtagtacgat
     7621 ggcgaaactg catgattact acaaagacga agtagttaaa aaactcatga ctgagtttaa
     7681 ctacaattct gtcatgcaag tccctcgggt cgagaagatc accctgaaca tgggtgttgg
     7741 tgaagcgatc gctgacaaaa aactgctgga taacgcagca gcagacctgg cagcaatctc
     7801 cggtcaaaaa ccgctgatca ccaaagcacg caaatctgtt gcaggcttca aaatccgtca
     7861 gggctatccg atcggctgta aagtaactct gcgtggcgaa cgcatgtggg agttctttga
     7921 gcgcctgatc actattgctg tacctcgtat ccgtgacttc cgtggcctgt ccgctaagtc
     7981 tttcgacggt cgtggtaact acagcatggg tgtccgtgag cagatcatct tcccagaaat
     8041 cgactacgat aaagtcgacc gcgttcgtgg tttggatatt accattacca ctactgcgaa
     8101 atctgacgaa gaaggccgcg ctctgctggc tgcctttgac ttcccgttcc gcaagtaagg
     8161 tagggttact aaatggctaa gcaatcaatg aaagcacgcg aagtaaaacg cgtagcttta
     8221 gctgataaat acttcgcgaa acgcgctgaa ctgaaagcga tcatctctga tgtgaacgct
     8281 tccgacgaag atcgttggaa cgctgttctc aagctgcaga ctctgccgcg tgattccagc
     8341 ccgtctcgtc agcgtaaccg ctgccgtcaa acaggtcgtc cgcatggttt cctgcggaag
     8401 ttcgggttga gccgtattaa ggtccgtgaa gccgctatgc gcggtgaaat cccgggtctg
     8461 aaaaaggcta gctggtaatt gtcaccaatt gaatcacggg agtaaagaca gatgagcatg
     8521 caagatccga tcgcggatat gctgacccgt atccgtaacg gtcaggccgc gaacaaagct
     8581 gcggtcacca tgccttcctc caagctgaaa gtggcaatcg ccaacgtgct gaaggaagaa
     8641 ggttttattg aagattttaa agttgaaggc gacaccaagc ctgaactgga acttactctg
     8701 aagtatttcc agggcaaagc tgttgtagaa agcattcagc gtgtcagccg cccaggtctg
     8761 cgcatctata aacgtaaaga tgagctgccg aaagttatgg cgggtctggg tatcgcagtt
     8821 gtttctacct ctaaaggtgt tatgactgat cgtgcagcgc gccaggctgg tcttggtggc
     8881 gaaattatct gctacgtagc ctaatcggag gaaaaaatgt ctcgtgttgc taaagcaccg
     8941 gtcgttgttc ctgccggcgt tgacgtaaaa atcaacggtc aggttattac gatcaaaggt
     9001 aaaaacggcg agctgactcg tactctcaac gatgctgttg aagttaaaca tgcagataat
     9061 accctgacct tcggtccgcg tgatggttac gcagacggtt gggcacaggc tggtaccgcg
     9121 cgtgccctgc tgaactcaat ggttatcggt gttaccgaag gcttcactaa gaagctgcag
     9181 ctggttggtg taggttaccg tgcagcggtt aaaggcaatg tgattaacct gtctctgggt
     9241 ttctctcatc ctgttgacca tcagctgcct gcgggtatca ctgctgaatg tccgactcag
     9301 actgaaatcg tgctgaaagg cgctgataag caggtgatcg gccaggttgc agcggatctg
     9361 cgcgcctacc gtcgtcctga gccttacaaa ggcaaggggg ttcgttacgc cgacgaagtc
     9421 gtgcgtacca aagaggctaa gaagaagtaa ggtaacacta tggataagaa atctgctcgt
     9481 atccgtcgtg cgacccgcgc acgccgcaag ctccaggagc tgggcgcaac tcgcctggtg
     9541 gtacatcgta ccccgcgtca catttacgca caggtaattg caccgaacgg ttctgaagtt
     9601 ctggtagctg cttctactgt agaaaaagct atcgctgaac aactgaagta caccggtaac
     9661 aaagacgcgg ctgcagctgt gggtaaagct gtcgctgaac gcgctctgga aaaaggcatc
     9721 aaagatgtat cctttgaccg ttccgggttc caatatcatg gtcgtgtcca ggcactggca
     9781 gatgctgccc gtgaagctgg ccttcagttc taaggtagag gtgtaagatg gctcacatcg
     9841 aaaaacaagc tggcgaactg caggaaaagc tgatcgcggt aaaccgcgta tctaaaaccg
     9901 ttaaaggtgg tcgtattttc tccttcacag ctctgactgt agttggcgat ggtaacggtc
     9961 gcgttggttt tggttacggt aaagcgcgtg aagttccagc agcgatccag aaagcgatgg
    10021 aaaaagcccg tcgcaatatg attaacgtcg cgctgaataa cggcactcta caacaccctg
    10081 ttaaaggtgt tcacacgggt tctcgcgtat tcatgcagcc ggcttccgaa ggtaccggta
    10141 tcatcgccgg tggtgcaatg cgcgccgttc tggaagtcgc tggggttcat aacgttctgg
    10201 ctaaagcata tggttccacc aacccgatca acgtggttcg tgcaactatt gatggcctgg
    10261 aaaatatgaa ttctccagaa atggtcgctg ccaagcgtgg taaatccgtt gaagaaattc
    10321 tggggaaata aaccatggca aagactatta aaattactca aacccgcagt gcaatcggtc
    10381 gtctgccgaa acacaaggca acgctgcttg gcttgggtct gcgtcgtatt ggtcacaccg
    10441 tagagcgcga ggatactcct gctattcgcg gtatgatcaa cgcggtttcc ttcatggtta
    10501 aagttgagga gtaagagatg cgtttaaata ctctgtctcc ggccgaaggc tccaaaaagg
    10561 cgggtaaacg cctgggtcgt ggtatcggtt ctggcctcgg taaaaccggt ggtcgtggtc
    10621 acaaaggtca gaagtctcgt tctggcggtg gcgtacgtcg cggtttcgag ggtggtcaga
    10681 tgcctctgta ccgtcgtctg ccgaaattcg gcttcacttc tcgtaaagca gcgattacag
    10741 ccgaagttcg tctatctgac ctggctaaag tagaaggcgg tgttgtagac ctgaacacgc
    10801 tgaaagcggc taacattatc ggtatccaga tcgagttcgc gaaagtgatc ctggctggcg
    10861 aagtaacgac tccggtaact gttcgtggcc tgcgtgttac taaaggcgct cgtgctgcta
    10921 tcgaagctgc tggcggtaaa atcgaggaat aagtagcaga tggctaaaca accgggatta
    10981 gattttcaaa gtgccaaagg tggcttaggc gagctgaaac gcagactgct gtttgttatc
    11041 ggtgcgctga ttgtgttccg tattggctct tttattccga tccctggtat tgatgccgct
    11101 gtacttgcca aactgcttga gcaacagcga ggcaccatca ttgagatgtt taacatgttc
    11161 tctggtggtg ctctcagccg tgcttctatc tttgctctgg ggatcatgcc gtatatttcg
    11221 gcgtcgatca ttatccagct gctgacggtg gttcacccaa cgttggcaga aattaagaaa
    11281 gaaggggagt ctggtcgtcg taagatcagc cagtacaccc gttacggtac tctggtgctg
    11341 gcaatattcc agtcgatcgg tattgctacc ggtctgccga atatgcctgg tatgcaaggc
    11401 ctggtgatta acccgggctt tgcattctac ttcaccgctg ttgtaagtct ggtcacagga
    11461 acgatgttcc tgatgtggtt gggcgaacag atcactgaac gaggtatcgg caacggtatt
    11521 tcaatcatta tcttcgccgg tattgtcgcg ggactcccgc cagccattgc ccatactatc
    11581 gagcaagcgc gtcaaggcga cctgcacttc ctcgtgttgc tgttggttgc agtattagta
    11641 tttgcagtga cgttctttgt tgtgtttgtt gagcgtggtc aacgccgcat tgtggtaaac
    11701 tacgcgaaac gtcagcaagg tcgtcgtgtc tatgctgcac agagcacaca tttaccgctg
    11761 aaagtgaata tggcgggggt aatcccggca atcttcgctt ccagtattat tctgttcccg
    11821 gcgaccatcg cgtcatggtt cgggggcggt actggttgga actggctgac aacaatttcg
    11881 ctgtatttgc agcctgggca accgctttat gtgttactct atgcgtctgc aatcatcttc
    11941 ttctgtttct tctacacggc gttggttttc aacccgcgtg aaacagcaga taacctgaag
    12001 aagtccggtg catttgtacc aggaattcgt ccgggagagc aaacggcgaa gtatatcgat
    12061 aaagtaatga cccgcctgac cttggttggt gcgctgtata ttacctttat ctgcctgatc
    12121 ccggagttca tgcgtgatgc aatgaaagta ccgttctact tcggtgggac ctcactgctt
    12181 atcgttgttg tcgtgattat ggactttatg gctcaagtgc aaactctgat gatgtccagt
    12241 cagtatgagt ctgcattgaa gaaggcgaac ctgaaaggct acggccgata attggtcgcc
    12301 cgagaagtta cggagagtaa aaatgaaagt tcgtgcttcc gtcaagaaat tatgccgtaa
    12361 ctgcaaaatc gttaagcgtg atggtgtcat ccgtgtgatt tgcagtgccg agccgaagca
    12421 taaacagcgc caaggctgat tttttcgcat atttttcttg caaagttggg ttgagctggc
    12481 tagattagcc agccaatctt ttgtatgtct gtacgtttcc atttgagtat cctgaaaacg
    12541 ggcttttcag catggaacgt acatattaaa tagtaggagt gcatagtggc ccgtatagca
    12601 ggcattaaca ttcctgatca taagcatgcc gtaatcgcat taacttcgat ttatggcgtc
    12661 ggcaagaccc gttctaaagc catcctggct gcagcgggta tcgctgaaga tgttaagatc
    12721 agtgagctgt ctgaaggaca aatcgacacg ctgcgtgacg aagttgccaa atttgtcgtt
    12781 gaaggtgatc tgcgccgtga aatcagcatg agcatcaagc gcctgatgga tcttggttgc
    12841 tatcgcggtt tgcgtcatcg tcgtggtctc ccggttcgcg gtcagcgtac caagaccaac
    12901 gcacgtaccc gtaagggtcc gcgcaaaccg atcaagaaat aatcggggtg attgaataat
    12961 ggcaaaggca ccaattcgtg cacgtaaacg tgtaagaaaa caagtctctg acggcgtggc
    13021 tcatatccat gcttctttca acaacaccat cgtgactatc actgatcgtc agggtaacgc
    13081 gttgggttgg gcaacagccg gtggttccgg tttccgtggt tctcgcaaat ccactccgtt
    13141 tgcagctcag gttgcagcag agcgttgcgc tgacgccgtg aaagaatacg gcatcaagaa
    13201 tctggaagtt atggttaaag gtccgggtcc aggccgcgaa tctactattc gtgctctgaa
    13261 cgccgcaggt ttccgcatca ctaacattac tgatgtgact ccgatccctc ataacggttg
    13321 tcgtccgccg aaaaaacgtc gcgtataacg cctcgttttc caggtttgtt ggagaaagaa
    13381 aatggcaaga tatttgggtc ctaagctcaa gctgagccgt cgtgagggca ccgacttatt
    13441 ccttaagtct ggcgttcgcg cgatcgatac caagtgtaaa attgaacaag ctcctggcca
    13501 gcacggtgcg cgtaagccgc gtctgtctga ctatggtgtg cagttgcgtg aaaagcaaaa
    13561 agttcgccgt atctatggtg tgctggagcg tcagttccgt aactactaca aagaagcagc
    13621 acgtctgaaa ggcaacaccg gtgaaaacct gttggctctg ctggaaggtc gtctggacaa
    13681 cgttgtatac cgtatgggct tcggtgccac tcgtgcagaa gcacgtcagc tggttagcca
    13741 taaagcaatt atggtaaacg gtcgtgttgt taacatcgct tcttatcagg ttagtccgaa
    13801 tgacgttgta agcattcgtg agaaagcgaa gaagcagtct cgcgtgaaag ccgctctgga
    13861 gctggctgag cagcgtgaaa agccaacttg gctggaagtt gatgctggca agatggaagg
    13921 tacgtttaag cgtaagccgg agcgttctga tctgtctgcg gacattaacg aacacctgat
    13981 cgtcgagctt tactccaagt aaagcttagt accaaagaga ggacacaatg cagggttctg
    14041 tgacagagtt tctaaaaccg cgcctggttg atatcgagca agtgagttcg acgcacgcca
    14101 aggtgaccct tgagccttta gagcgtggct ttggccatac tctgggtaac gcactgcgcc
    14161 gtattctgct ctcatcgatg ccgggttgcg cggtgaccga ggttgagatt gatggtgtac
    14221 tacatgagta cagcaccaaa gaaggcgttc aggaagatat cctggaaatc ctgctcaacc
    14281 tgaaagggct ggcggtgaga gttcagggca aagatgaagt tattcttacc ttgaataaat
    14341 ctggcattgg ccctgtgact gcagccgata tcacccacga cggtgatgtt gaaatcgtca
    14401 agccgcagca cgtgatctgc cacctgaccg atgagaacgc gtctattagc atgcgtatca
    14461 aagttcagcg cggtcgtggt tatgtgccgg cttctacccg aattcattcg gaagaagatg
    14521 agcgcccaat cggccgtctg ctggtcgacg catgctacag ccctgtggag cgtattgcct
    14581 acaatgttga agcagcgcgt gtagaacagc gtaccgacct ggacaagctg gtcatcgaaa
    14641 tggaaaccaa cggcacaatc gatcctgaag aggcgattcg tcgtgcggca accattctgg
    14701 ctgaacaact ggaagctttc gttgacttac gtgatgtacg tcagcctgaa gtgaaagaag
    14761 agaaaccaga gttcgatccg atcctgctgc gccctgttga cgatctggaa ttgactgtcc
    14821 gctctgctaa ctgccttaaa gcagaagcta tccactatat cggtgatctg gtacagcgta
    14881 ccgaggttga gctccttaaa acgcctaacc ttggtaaaaa atctcttact gagattaaag
    14941 acgtgctggc ttcccgtgga ctgtctctgg gcatgcgctt ggaaaactgg ccaccggcaa
    15001 gcatcgctga cgagtaaccg gatcacaggt taaggtttta ctgagaagga taaggtcatg
    15061 cgccatcgta agagtggtcg tcaactgaac cgcaacagca gccatcgcca ggctatgttc
    15121 cgcaatatgg caggttcact ggttcgtcat gaaatcatca agacgactct gcctaaagcg
    15181 aaagagctgc gccgcgtagt tgagccgctg attactcttg ccaagactga tagcgttgct
    15241 aatcgtcgtc tggcattcgc ccgtactcgt gataacgaga tcgtggcaaa actgtttaac
    15301 gaactgggcc cgcgtttcgc gagccgtgcc ggtggttaca ctcg
//
LOCUS       DAJTRC010000088        14491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.088, whole genome shotgun sequence.
ACCESSION   DAJTRC010000088 DAJTRC010000000
VERSION     DAJTRC010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.088"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(114..380)
                     /locus_tag="OO357_003058"
     CDS             complement(114..380)
                     /locus_tag="OO357_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015964583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HCR5887922.1"
                     /translation="MRAAFWVGCAALLLSACSSEPVQQATAAHVAPSLKASMSSSGEA
                     NCAMIGGSLSVARQLDGTAIGMCALPNGKRCSEQSLAAGSCGSY"
     gene            654..4178
                     /gene="nifJ"
                     /locus_tag="OO357_003059"
     CDS             654..4178
                     /gene="nifJ"
                     /locus_tag="OO357_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HCR5887923.1"
                     /translation="MITIDGNGAVASVTFRISEVIAIYPITPSSTMAEQADAWAGNGL
                     KNVWGDTPRVVEMQSEAGAIATVHGALQTGALSTSFTSSQGLLLMIPTLYKLAGELTP
                     FVLHVAARTVATHALSIFGDHSDVMAVRQTGCAMLCAANVQEAQDFALISHIATLKSR
                     VPFIHFFDGFRTSHEINKIVPLADDTILDLMPQAEIDAHRARALNPEHPVIRGTSANP
                     DTYFQSREATNPWYNAVYDHVEQAMNDFAAATGRQYQPFEYYGHPQAERVIILMGSAI
                     GTCEEVVDELLTRGEKVGVLKVRLYRPFSAKHLLQALPGSVRSVAVLDRTKEPGAQAE
                     PLYLDVMTALAEAFNNGERETLPRVIGGRYGLSSKEFGPDCVLAVFAELNAAKPKARF
                     TVGIYDDVTNLSLPLPENTLPNSAKLEALFYGLGSDGSVSATKNNIKIIGNSTPWYAQ
                     GYFVYDSKKAGGLTVSHLRVSEQPIRSAYLISQADFVGCHQLQFIDKYQMAERLKPSG
                     IFLLNTPYSADEVWSRLPQEVQAVLNQKKARFYVINAAKIARECGLAARINTVMQMAF
                     FHLTQILPGDSALAELQGAIAKSYSSKGQDLVERNWQALALARESVEEVPLQPVNPHS
                     ANRPPVVSDAAPDFVKTVTAAMLAGLGDALPVSALPPDGTWPMGTTRWEKRNIAEEIP
                     IWKEELCTQCNHCVAACPHSAIRAKVVPPEAMENAPASLHSLDVKSRDMRGQKYVLQV
                     APEDCTGCNLCVEVCPAKDRQNPEIKAINMMSRQEHVEEEKINYDFFLNLPEIDRSKL
                     ERIDIRTSQLITPLFEYSGACPGCGETPYIKLLTQLYGDRMLIANATGCSSIYGGNLP
                     STPYTTDANGRGPAWANSLFEDNAEFGLGFRLTVDQHRVRVLRLLDQFADKIPAELLT
                     ALKSDATPEVRREQVAALRQQLNDVAEAHELLRDADALVEKSIWLIGGDGWAYDIGFG
                     GLDHVLSLTENVNILVLDTQCYSNTGGQASKATPLGAVTKFGEHGKRKARKDLGVSMM
                     MYGHVYVAQISLGAQLNQTVKAIQEAEAYPGPSLIIAYSPCEEHGYDLALSHDQMRQL
                     TATGFWPLYRFDPRRADEGKLPLALDSRPPSEALEETLLHEQRFRRLNSQQPEVAEQL
                     WKDAAANLQKRYDFLAQMAGKAEKSNTD"
     gene            4545..5674
                     /gene="ompN"
                     /locus_tag="OO357_003060"
                     /pseudo
     CDS             4545..5674
                     /gene="ompN"
                     /locus_tag="OO357_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415895.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpN"
     gene            5815..6249
                     /gene="uspF"
                     /locus_tag="OO357_003061"
     CDS             5815..6249
                     /gene="uspF"
                     /locus_tag="OO357_003061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspF"
                     /protein_id="HCR5887924.1"
                     /translation="MNRTILVPIDISDSELTQRVISHVEAEAKIDDAEVHFLTVIPSL
                     PYYASLGLAYSAELPAMDDLKAEAKSQLEEIIKKFKLPTDRVHVHVEEGSPKDRILEL
                     AKKIPAHMIIIASHRPDITTYLLGSNAAAVVRHAECSVLVVR"
     gene            6425..7360
                     /gene="ttcA"
                     /locus_tag="OO357_003062"
     CDS             6425..7360
                     /gene="ttcA"
                     /locus_tag="OO357_003062"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiocytidine(32) synthetase TtcA"
                     /protein_id="HCR5887925.1"
                     /translation="MSQNQEISKKEQYNLNKLQKRLRRNVGEAIADFNMIEEGDRIMV
                     CLSGGKDSYTMLEILRNLQQSAPINFSLVAVNLDQKQPGFPEHVLPEYLEKLGVEYKI
                     VEENTYGIVKEKIPEGKTTCSLCSRLRRGILYRTATELGTTKIALGHHRDDILQTLFL
                     NMFYGGKMKGMPPKLMSDDGKHIVIRPLAYCREKDIQRFADAKAFPIIPCNLCGSQPN
                     LQRQVIADMLRDWDKRYPGRIETMFSAMQNVVPSHLCDTNLFDFKGITHGSEVVNGGD
                     LAFDREEIPLQPAGWQPEEDENQLDELRLNVVEVK"
     gene            complement(7489..8856)
                     /gene="dbpA"
                     /locus_tag="OO357_003063"
     CDS             complement(7489..8856)
                     /gene="dbpA"
                     /locus_tag="OO357_003063"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase DbpA"
                     /protein_id="HCR5887926.1"
                     /translation="MTAFSTLNVLPPAQLTNLNELGYLTMTPVQAAALPAILAGKDVR
                     VQAKTGSGKTAAFGLGLLQQIDASLFQTQALVLCPTRELADQVAGELRRLARFLPNTK
                     ILTLCGGQPFGMQRDSLQHAPHIIVATPGRLLDHLQKGTVSLDALNTLVMDEADRMLD
                     MGFSDAIDDVIRFAPASRQTLLFSATWPEAIAAISGRVQRDPLAIEIDSTDALPPIEQ
                     QFYETSSKGKIPLLQRLLSLHQPSSCVVFCNTKKDCQAVCDALNEVEQSALSLHGDLE
                     QRDQTLVRFANGSARVLVATDVAARGLDIKSLELVVNFELAWDPEVHVHRIGRTARAG
                     NSGLAISFCAPEEAQRANIISDMLQIKLNWQTPPANSSIVTLEAEMATLCIDGGKKAK
                     MRPGDVLGALTGDIGLDGADIGKIAVHPAHVYVAVRQAVAHKAWKQLQGGKIKGKTCR
                     VRLLK"
     gene            complement(9334..10317)
                     /gene="zntB"
                     /locus_tag="OO357_003064"
     CDS             complement(9334..10317)
                     /gene="zntB"
                     /locus_tag="OO357_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc transporter ZntB"
                     /protein_id="HCR5887927.1"
                     /translation="MEAIKGSDVNVPDAVFAWMLDGRGGVKPLENTDVIDEAHPCWLH
                     LNYVHHDSAQWLATTPLLPNNVRDALAGESTRPRVSRFGEGTLITLRCINGSTDERPD
                     QLVAMRVYMDGRLIVSTRQRKVLALDDVVSDLEEGTGPTDCGGWLVDVCDALTDHSSE
                     FIEQLHDKIIDLEDNLLDQQIPPRGFLALLRKQLIVMRRYMAPQRDVYARLASERLPW
                     MSDDQRRRMQDIADRLGRGLDEIDACIARTGVMADEIAQVMQENLARRTYTMSLMAMV
                     FLPSTFLTGLFGVNLGGIPGGGWQFGFSIFCILLVVLIGGVALWLHRSKWL"
     gene            10572..11804
                     /gene="dgcM"
                     /locus_tag="OO357_003065"
                     /pseudo
     CDS             10572..11804
                     /gene="dgcM"
                     /locus_tag="OO357_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309952.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcM"
     gene            complement(11825..12388)
                     /gene="smrA"
                     /locus_tag="OO357_003066"
                     /pseudo
     CDS             complement(11825..12388)
                     /gene="smrA"
                     /locus_tag="OO357_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA endonuclease SmrA"
     gene            complement(12642..14108)
                     /locus_tag="OO357_003067"
     CDS             complement(12642..14108)
                     /locus_tag="OO357_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HCR5887928.1"
                     /translation="MMSMRKHIAFASMCMGLFIAQLDIQIVSSSLNEIGGGLSAGKDE
                     MAWLQTSYLIAEIIVIPLSGWLSRVFSTRWLFTLSAGIFTLMSIACGIACGLAWNIQI
                     MILFRALQGAAGASMIPLVFTMAFIYYQGKELGLAAAVVSALASLSPTLGPTLGGWLT
                     DNLDWRWLFYINILPGIYLVLSIPFLVNFDKPDLSLLKVADYPSIILLAMTLGCLEYT
                     LEEGARWGWLDDNTILLTSVLALVSFILFAARTLTISNPIMDLHAFKDKNFTLGCFFS
                     FSGGVGIFSTVYLIPVFLGQIRGLNAEEIGFAVCTTGIFQLFSVPFYFWLSKKINLRW
                     LLMAGLGGFVFSMYLFTPITHEWGWQELLFPQAIRGISQQFAMAPIVTLTLGGIPKER
                     LKLASGVFNLTRNLGGAIGIALCGSILNNRTNFHFSRMGEKMVSVPHTVNDFISRSAL
                     FFNRSRSDQTSEILASTKLLSQLMLREAQTMAFSVPFC"
     gene            complement(14105..>14491)
                     /locus_tag="OO357_003068"
     CDS             complement(14105..>14491)
                     /locus_tag="OO357_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5887929.1"
                     /translation="APIDGIVANRSAHTGSWVEGGTSLVSLVPVSELWVDANYKENQI
                     AGMKPGMKAEIRADILKGEVFHGHIESLSPATGASFSLIPIENATGNFTKIVQRVPVR
                     IAFDDAKELKQLLRPGLSVTVSVDER"
BASE COUNT         3863 a         3656 c         3419 g         3553 t
ORIGIN      
        1 aacaccatta gcgaaatgct gaaagaaggt gcacaagtgg atctgaccgc gaaccagtta
       61 acgctggcga ccgcgaaaca gacattaact tataagctgg cggatttaat gaattaatag
      121 ctgccacagc tcccggcggc aagtgactgt tcgctacagc gtttgccgtt gggtaatgca
      181 cacatcccaa tcgccgtacc atccagttga cgggcaacag aaagcgaacc gccgatcatt
      241 gcacaatttg cttctccact actggacatc gacgctttta aacttggcgc tacgtgcgcc
      301 gcagtcgcct gctgaacagg ctcactacta cacgccgaca acaataaagc ggcacaccct
      361 acccaaaacg ctgctcgcat ctcttcttcc tctgatcttc aagccaaacg acaccgccat
      421 aaataatagg cagcacagag ggcggcgtcg agagctgtcc tgcgcgttgc cccgcatttt
      481 tactttttta tggctatttt tttgccctct gtttgatcaa aacattcatt actctgatgt
      541 gggggacaca aaagcgaaaa tgcagaagaa agccatttgc taaaattgaa ggattactac
      601 tgggcgcgca gcaatttcgt gcgcccctca tttgcgcaat gtaagggtgt catatgatta
      661 ctattgacgg taatggcgcg gttgcttcgg tgacgtttcg catcagtgaa gttatcgcca
      721 tctaccctat tacccccagt tccacgatgg cagaacaggc tgatgcctgg gccggaaacg
      781 gcttgaagaa cgtttgggga gacacaccac gcgtggttga aatgcagtcg gaagcgggtg
      841 ctatcgctac cgtgcatggc gctttgcaga cgggtgccct ttcaacatcg tttacgtcat
      901 cgcagggttt gctgctgatg atcccgacgc tgtacaaact ggcaggcgaa ctgacgccat
      961 ttgtcctgca tgtagcggca cgtaccgttg ccacacatgc actttctatt tttggcgatc
     1021 attccgacgt tatggcggtg cgccagacgg gttgcgcgat gttgtgtgcg gctaacgtcc
     1081 aggaagcgca agactttgct ctcatttcgc acatcgcaac gctgaagagc cgcgtgccat
     1141 ttattcattt ctttgatggt tttcgcacgt cccacgaaat caataaaatt gtcccgctgg
     1201 ccgatgacac tattcttgat ctcatgccgc aggccgaaat tgatgctcat cgcgcccggg
     1261 cgctcaaccc ggaacatccg gtgatccgcg gtacgtccgc caatcctgac acttatttcc
     1321 agtctcgcga agccaccaat ccctggtaca acgcggtcta tgaccatgtt gaacaggcga
     1381 tgaatgattt cgctgccgcg acaggtcgtc agtatcagcc gtttgagtat tacgggcatc
     1441 cgcaagcgga acgggtgatt atcctgatgg gctctgccat tggcacctgt gaagaagtgg
     1501 ttgatgaatt gctaacccgt ggcgaaaaag ttggcgtgct gaaagttcgt ctgtaccgtc
     1561 ccttctccgc caaacacttg ctgcaagctc tgccgggatc cgtacgcagc gtggcggtac
     1621 tggacagaac caaagaaccc ggtgcccagg cagaaccgct ctatctggat gtgatgaccg
     1681 cactggcaga agcctttaat aatggtgagc gcgaaaccct gccccgtgtc attggtgggc
     1741 gctatggtct ttcatccaaa gaatttggcc cggactgtgt actggcggta tttgccgagc
     1801 tcaacgcggc taaacctaaa gcacgcttta cggttggtat ttacgatgat gtgaccaatc
     1861 tgtcactgcc gttgccggaa aacactctgc caaactcggc gaaacttgaa gccttgtttt
     1921 atggccttgg tagtgatggc agcgtttccg caaccaaaaa caatatcaag attatcggta
     1981 attccacgcc gtggtacgca cagggctatt ttgtttacga ctccaaaaaa gcgggcggcc
     2041 tgacggtttc tcaccttcgc gtgagcgaac agccgattcg ttccgcttat ctcatttccc
     2101 aggctgattt tgttggctgc caccagttgc agtttatcga taaatatcag atggctgagc
     2161 gtttaaaacc tagcggcatt ttcctgctta atacgccgta cagcgcagat gaagtgtggt
     2221 cgcgcttgcc gcaggaagtt caggccgtgt taaaccagaa aaaagcgcgc ttctatgtga
     2281 ttaacgcggc gaaaatcgcc cgcgaatgtg gcctggcggc ccgtattaat accgtcatgc
     2341 agatggcttt tttccatctg acgcaaattc tgcctggcga tagcgccctc gcagaattgc
     2401 agggtgcgat tgccaaaagt tacagtagca aaggtcagga tctggtggag cgcaactggc
     2461 aggctctggc gctggcgcgt gaatccgtag aagaagttcc gttgcaaccg gtaaatccgc
     2521 acagcgccaa tcgaccgcca gtggtttccg atgccgcccc tgatttcgtg aaaaccgtaa
     2581 ccgcggcgat gctcgccggg cttggtgatg ccctccccgt ttcggcgctg ccgccagacg
     2641 gcacctggcc gatgggcacc actcgctggg aaaaacgcaa tatcgccgaa gagatcccca
     2701 tctggaaaga ggaactctgt acccaatgta accactgcgt cgccgcttgc ccacactcag
     2761 ctattcgcgc aaaagtggta ccgcctgaag cgatggaaaa cgcccctgcc agcctgcatt
     2821 cgctagatgt gaaatcgcgt gatatgcgcg ggcagaaata tgtcttgcag gtggcaccgg
     2881 aagattgcac cggttgtaac ctgtgcgtcg aagtttgccc ggcgaaagac cgtcagaatc
     2941 cagagattaa agccatcaat atgatgtctc gccaggaaca tgtcgaagaa gagaaaatca
     3001 attacgattt cttcctcaac ctgccagaaa tcgaccgtag caaactggaa cgtattgata
     3061 ttcgtacatc gcagctgatt acaccgctgt ttgaatattc aggtgcttgc cccggttgtg
     3121 gcgagacgcc gtatattaaa ttgctgactc agctctatgg tgaccggatg ttgatcgcta
     3181 acgccaccgg ctgttcttca atttatggcg gtaacctgcc ctctacaccg tataccaccg
     3241 atgccaacgg tcgtgggccg gcatgggcga actctctatt tgaagataat gccgaatttg
     3301 gccttggttt ccgcctgacg gtcgatcaac accgtgtccg cgtgctgcgt ctgctggatc
     3361 aatttgccga taaaatcccg gcggaattac tgacggcgtt gaaatcagac gctacgccag
     3421 aggttcgtcg agaacaggtc gcagctttac gccagcaact caacgatgtt gccgaagcac
     3481 atgaacttct gcgtgacgca gatgcactgg tggaaaaatc aatctggctg attggtggcg
     3541 acggctgggc ttacgatatc ggctttggcg gtctggatca tgtattgagt ttgacggaaa
     3601 acgtcaacat tctggtgctg gatacgcaat gctattccaa caccggtggt caggcgtcga
     3661 aagcgacacc gctgggtgca gtaactaagt tcggcgagca cggcaaacgt aaagcgcgta
     3721 aagatcttgg cgtcagtatg atgatgtacg gtcatgttta tgtggcgcag atttctctcg
     3781 gcgcgcagct gaaccagacg gtgaaagcga ttcaggaagc ggaagcgtat ccgggaccat
     3841 cgctgatcat tgcttatagc ccgtgtgaag agcatggtta cgatctggct ctcagccacg
     3901 accagatgcg ccaactcact gctaccggct tctggccgct atatcgcttt gatccgcgtc
     3961 gtgccgatga aggcaaactg ccgctggcgc tggattcacg cccgccgtca gaagcactgg
     4021 aagaaacgtt acttcacgag cagcgtttcc gtcgtctgaa ttcgcagcag ccagaagtgg
     4081 cagaacagtt atggaaagat gctgcagcta atttgcaaaa acgctatgac ttcctagcac
     4141 aaatggccgg aaaagcggaa aaaagcaaca ccgattaaat gctctggata aggattatcc
     4201 aattctaaaa aaaaagcccg gacgactgtt cgggcttgtc tttttatatg ttggaaaatc
     4261 agtggcaatg caatggccca acagaaaata aaatctgtga caataaaggc atataacccg
     4321 cgcagaataa cgtataggaa attaaaagta taatttttat tttgtatgta ttatcttcga
     4381 tacattcacc agacttatta tcatttcaga ggaattattt gattaaggtt ttacttaagg
     4441 cgtaacaaat gatatttatc ggctaactga acttctcctt taggatgttt tcactccccg
     4501 aaatggggat aaaaggcaaa taaaataact aaaggattta ttcaatgaaa agaaaagtac
     4561 tggcactttt aattcctgcc ctgctcgccg caggtgctgc acatgcagcc gaagtttata
     4621 ataaagacgg caacaaatta gatctgtatg gcaaagttga tggcctgcat tatttttctg
     4681 ataattcagc gaaagatggc gaccagagct atgcgcgtct gggttttaaa ggcgaaaccc
     4741 aaattaacga tcaacttact ggctacggtc aatgggaata caatattcag gcaaacaaca
     4801 ctgaatcttc aaaaaaccag tcatggaccc gtctggcatt tgccggactg aaatttgcag
     4861 attacggttc tttcgattac ggacgtaatt atggcgtaat gtacgacatc gaaggctgga
     4921 ccgatatgct gcctgaattt ggcggtgact cttataccaa tgcagacaac tttatgactg
     4981 gtcgagccaa tggcgtcgcg acttatcgta atactgattt cttcggtctg gtaaatggtc
     5041 tgaacttcgc ggtgcagtat caaggtaaca acgaaggagc cagtaatggt caggaaggca
     5101 ccaataacga acgcgatgtt cgtcatgaaa acggtgacgg ctggggtctt tccacaacat
     5161 atgatttagg catgggcttt agcgctggtg cggcatacac ctcttctgac cgcaccaatg
     5221 accaggttaa ccatactgcg gcgggtggtg ataaagcaga cgcgtggact gctgggctaa
     5281 aatacgatgc taacaatatt tacctagcaa ccatgtattc agaaacgcgt aatatgaccc
     5341 cgtttggcga cagcgattat gcagttgcga acaaaaccca gaactttgaa gttactagta
     5401 ccagtttgat ttttgtctgc gtccggcagt ctctttcctg atgtctaaag gccgtgacct
     5461 tcacgctgct ggtggtgcag acaacccggc aggtgttgat gataaagatc tggttaaata
     5521 cgccgatgtt ggcgcgactt actatttcaa taaaaacatg tccacctatg ttgactataa
     5581 aatcaacctg ttggatgaag atgacagttt ctacgctgcc aacggcatct ctaccgatga
     5641 tattgtcgct ttaggtctgg tttatcagtt ctaaatcctc ctgcccgctg ttatggcggg
     5701 ctttttctgc ttattcttcc tcctttgatc taaattaaaa atgtgaactc cgtcattaca
     5761 caaaaagtgt catctggcgt tacactttat gcggatacta aaacaggagg ttttatgaac
     5821 agaacgattc ttgtccctat cgatatttca gattcagaat taactcaacg cgtgattagc
     5881 cacgttgagg cagaggcaaa gattgatgat gcagaggttc atttcctgac ggtaatacct
     5941 tcactgccct actatgcctc tctgggttta gcgtattccg cagaattacc ggcaatggat
     6001 gacctgaaag cggaagccaa atcgcaactg gaagagatca ttaaaaaatt taaactgcca
     6061 accgacagag tgcatgtcca tgttgaggaa ggctcgccca aagaccgcat tctggaattg
     6121 gcgaagaaga tccccgctca tatgatcatc attgcttccc atcgaccgga tatcaccact
     6181 tatctgctcg gttccaacgc cgcagctgta gtgcgtcacg cagagtgctc cgtgctggtt
     6241 gtgcgctgac actaacgccc gcacattgct gcgggctttt tgattcattt cgcaaatgtg
     6301 ctgacatttt cccctctaat ccgtaccata cacgccacag tttttatatc agatttctta
     6361 tgctgagcgt tccggcatga cgcatactct tctgatgcca tataacgaat tgagtcgctt
     6421 ttaaatgtcg caaaatcaag aaattagtaa gaaagaacaa tacaacctga acaaattaca
     6481 aaaacgtctg cgtcgtaacg tgggcgaagc cattgctgac ttcaatatga ttgaagaagg
     6541 cgatcgcatc atggtttgcc tctccggggg taaagacagc tataccatgc tggagattct
     6601 gcgcaatttg cagcaaagcg cgccaatcaa tttttcgctg gtggctgtta acctcgatca
     6661 aaagcaaccg ggcttcccgg aacacgttct gcccgagtat cttgaaaagc tgggcgttga
     6721 gtacaagatt gttgaagaga acacttacgg tatcgtgaaa gagaagattc cagagggcaa
     6781 aaccacttgc tcactgtgtt ctcggctacg tcgcggtatc ctttatcgca ccgcaacgga
     6841 actggggacg acgaagatcg cgttgggtca ccatcgtgac gatatcctgc aaacgttgtt
     6901 cttaaatatg ttctacggcg gcaagatgaa aggtatgcct ccgaaactga tgagcgatga
     6961 tggcaaacat atcgttattc gtccactggc ctactgccgc gagaaagata ttcagcgatt
     7021 tgccgatgca aaagcgttcc cgattattcc gtgcaacctg tgcggttcac agcctaacct
     7081 gcaacgtcag gtgattgctg acatgttgcg tgactgggat aaacgttatc cagggcgtat
     7141 cgagacgatg ttcagcgcga tgcagaatgt ggtgccgtcg catctgtgcg ataccaacct
     7201 gttcgatttc aaaggcatta cccacggttc tgaagtggtt aacgggggtg atctggcgtt
     7261 tgatcgcgaa gagatcccac tacaaccggc gggctggcag ccagaagaag atgaaaatca
     7321 gttggatgag ttacggctga atgtggttga agtgaaataa ccaggatagc gcccgatgcg
     7381 caagcgtatc gggctactct tatggaggcc ggataagacg cggccagcgt cgcatctggc
     7441 aatcccgaat aagatgttta ctcttgcacc cggcaattca acatttcatt attttaataa
     7501 ccgcacccgg cacgtttttc ctttaatctt cccgccctgt aactgtttcc atgctttatg
     7561 agcaacagcc tgacggaccg cgacatagac atgcgccgga tgcacggcga ttttgccaat
     7621 atctgcgcca tcaagcccga tatctcctgt cagtgcacct aatacatcac ccgggcgcat
     7681 tttggctttt ttcccgccat cgatacacaa cgttgccatt tctgcttcca gcgtcacaat
     7741 ggaactatta gctggcggcg tttgccagtt aagttttatc tgcaacatgt cagaaatgat
     7801 attggcccgc tgtgcttctt ccggagcaca gaaactgatc gccagaccgc tatttcctgc
     7861 acgagctgta cgaccgatgc gatgtacatg aacttcaggg tcccacgcca gctcaaagtt
     7921 caccaccagc tcaagcgatt taatatccag accacgcgca gcaacatcag tcgcaaccag
     7981 tacacgggcg ctaccgttag caaaacgtac cagggtctga tcgcgttgct ccagatcgcc
     8041 gtgtaacgac aatgcacttt gctctacttc attcagcgcg tcgcagacag cctggcaatc
     8101 ttttttggta ttgcaaaaca ccacacaaga ggatggctga tgcaagctta ataaccgttg
     8161 caacagagga attttgcctt tactggatgt ctcataaaat tgttgttcaa tgggtggcaa
     8221 agcatctgtt gagtcaattt caatcgccaa aggatcgcgt tgcactcgtc cgctgattgc
     8281 ggcgatggct tccggccagg ttgccgaaaa cagaagcgtc tgtcgagatg caggcgcaaa
     8341 acggatgaca tcatcaatgg catcgctaaa tcccatatcc agcatgcggt cggcctcatc
     8401 catcaccaaa gtattcagcg catccagtga taccgtgcct ttttgcaagt gatccagcaa
     8461 acgacccggc gttgccacga taatatgcgg cgcatgttgc agcgaatcac gctgcatacc
     8521 gaacggttga ccaccgcaca acgtcaaaat tttggtattt ggcagaaaac gcgccagccg
     8581 acgcaattca cctgccacct gatccgccag ttcacgcgta ggacacagca ctaaagcctg
     8641 ggtttgaaat agcgacgcat caatttgctg taacaagccg aggccaaaag ccgccgtttt
     8701 gccgctgccg gttttcgcct gcacgcgaac atcttttccg gcaaggatcg ccggaagcgc
     8761 ggcggcctgc accggcgtca tggttaaata acccaactca ttaaggttcg tgagttgggc
     8821 gggaggcaaa acattcaggg tagaaaaagc ggtcacaatc tattctcgtg gtcatcgacg
     8881 caaagttagc aggcgcgtat cctcgcagat ctacgctcac gatgcgacaa tttaatcggt
     8941 tcttcatcgg gtggtgggtc aggcatgggt tgcgggcgag ggatcggatc gggcactgga
     9001 acaggatcgc caggaatcgg ttcagggaca ggaatttgca aataaataag tgtcgtcata
     9061 tttccctccg gtcattgggt ggactcttaa agggtagacg ctgataaata acaggcaaaa
     9121 aaaagccgac tcatcaaagt cggcgtcgta cgaatcaatt gtgctatgca gtaattcaaa
     9181 aaaggaagta agacaatatg gagcgcaacg cccatcgctt gacgttgcat tcacctgcaa
     9241 gggagatatt gccctgaatg ggtagagagt ttattgactt cgctcaaact ttgcggcgtt
     9301 tttgtataca gacagccgga aaaattgctt tttttacaac catttactac ggtgcaacca
     9361 taaagcaaca ccaccaataa gaacaactaa cagaatacaa aaaattgaaa atccgaattg
     9421 ccacccgccg ccagggatcc caccaaggtt gacgccaaat aacccggtca gaaaggtact
     9481 gggtaaaaag accattgcca tcaacgacat tgtataggta cgacgagcta aattttcctg
     9541 catcacctga gcgatttcat ccgccatcac gccagtccgt gctatacagg cgtcgatttc
     9601 gtcaaggccg cgcccaaggc gatcggcaat atcctgcatc cgacggcgtt ggtcatcgct
     9661 catccacggc aaacgttcac tggcaagacg agcataaaca tcacgttgcg gtgccatata
     9721 gcgacgcatc acaattaatt gtttgcgcag cagagccagg aatccacgcg gtggaatttg
     9781 ctgatcaagg agattatctt caaggtcgat aattttatcg tgcagctgct cgataaattc
     9841 actggaatga tcggtcaacg catcgcacac atccaccagc catcccccgc aatcggtcgg
     9901 acccgtgccc tcttccagat cgctcaccac atcgtccagc gccagcactt tgcgttgtcg
     9961 ggtcgaaaca attaaccgcc cgtccatata tacacgcatg gcgaccagtt gatcggggcg
    10021 ttcatcggtg ctgccgttta tacagcgcaa tgtaatcagc gtgccttcac cgaaacggct
    10081 gactcggggc cgtgtgctct cgcccgccag cgcatcacgt acgttattgg gaagcagcgg
    10141 tgttgtcgcc agccattggg cgctatcatg gtgtacataa ttaaggtgga gccagcaggg
    10201 atgcgcttca tcaatcacat ctgtattttc cagcggttta acgccgcctc taccatccag
    10261 catccaggca aatactgcat ccgggacatt aacgtccgat cccttaatcg cttccactgt
    10321 gcctccatca tcaacgcatt attttgtagt ctagccttct ggccctgtta cgcaacatct
    10381 catcacccca ttaccctgaa atgattaata aaattctgtc taaattgaat acaaaaagca
    10441 aaatgctttt ccgtatacaa accgtgtgaa gtgttaaata gcgtctatca ttatcagaat
    10501 tatctgatca tatgacgtgg cttttttgcg atcggatagc aacaaaaatt gataaaaata
    10561 acgggatctc aatgattacg cacaacttca ataccctgga cttactcacc agtcctgtct
    10621 ggattgtttc gccctttgag gaacagttaa tttatgccaa tagcgcggcg cgactgttga
    10681 tgcaagacct cacgtttagt cagctacgaa ccggacccta ttccgtctcc tcacaaaaag
    10741 aactgccgaa atacctctcc gatctgcaaa accaacacga tattatcgaa atcctcactg
    10801 ttcagcgtaa agaagaggaa acagcattaa gctgtcggct tgttttgcga gagctgacag
    10861 aaacagaacc ggtgattatt ttcgaaggta tcgaagcgcc ggcaacgctg ggtttaaaag
    10921 ccagtcgctc ggcaaattat cagcgcaaaa aacaaggttt ttatgcgcgc ttttttctga
    10981 ctaactctgc accaatgttg ttgattgacc cgtcacgaga tggacaaatc gtcgatgcta
    11041 acctcgccgc gctcaatttc tatggttata accatgaaac gatgtgccag aaacatacct
    11101 aggaaataaa tatgcttggg cgtcgcgtca tgcctatcat gcatgaaatt tcgcatttac
    11161 ccggcggtca taaccctttg aattttgttc ataaactggc ggatggatct actcgtcatg
    11221 tgcagaccta tgccggaccg attgaaattt atggcgacaa gctcatgtta tgtattgtgc
    11281 atgatattac tgagcaaaaa cggctggagg agcagctgga acatgctgct caccatgacg
    11341 cgatgaccgg attactgaat cggcgacagt tttatcacat tacggaacca ggccaaatgc
    11401 agcatctcgc catcgctcag gattacagct tgttgctcat cgacaccgat cgttttaaac
    11461 acattaacga tctctatggg cattctaaag gtgatgaggt gttatgcgcc ctcgcccgcc
    11521 ccctcgaaag ttgcgctcgc aaaggcgatt tggtgtttcg ttggggaggc gaagagtttg
    11581 tcttattgct accaagaacc ccactggata ccgcgctttc gctggctgaa actatccgcg
    11641 taagcgtggc aaaagtgagt atttcgggct taccacgctt taccgtcagc attggtgtgg
    11701 cgcatcacga aggaaatgaa agcatcgatg aactgtttaa acgcgttgat gatgctttgt
    11761 atcgggcgaa aaatgatgga cgcaaccgcg tgctggcggc ataagccgcg gatgcgtctc
    11821 gagatcaacg actgcgctta gcgtggcgct cccagttttc ttgcttcgcc tgcgccgttt
    11881 tacgtagtgc gacgtaacac gccccgctgc cgccatgatg cggtagcgcg gtgcaatatg
    11941 cctgaacatc atcaaattcg gtcagccagc gcgccacata gctgcggaca atattggcat
    12001 gtgatttatc ttcccgccct ttaccatgaa taatcagcac attacgcaaa ccatccgcca
    12061 gggcttgttg aatgaaactg aacaccattt tgcggcactc ttccaccggc tggcgcaaaa
    12121 gattcaggct cgcctgttgc ggatatttac cactgcgcag cttatccagc accccatgct
    12181 acaacccttc ctgccgaaac tccagcggct gacttagcgg gatgatgtcg agaaatccgg
    12241 tagtgaggaa attatcaagc tgcagcgtgt cgatacgctg cggcgcacgt tggttacgcg
    12301 ttggatgcca gtggacatct gtagcacgtt tcagcggctg gacatcttcc atggcgtcaa
    12361 gaaacagcga tttgtcgtca aggttcatgt aacctcctat gcaccttcac acgtcgtgat
    12421 tcagacgggt taattgttca tacttactgc atcgttccct tcacagattg aaacaatact
    12481 ctacttcata cctgtgagta tctgcgcagg cggggcattt tatactagcc tgttagcagt
    12541 atcgaacagt ttagcccgag tttaattcag cacaggctcc gatcaggatg atttattcat
    12601 agcaggaacc aacaaaaatg cgataaataa cagaccactg attagcagaa aggtaccgaa
    12661 aatgccatag tttgcgcctc cctcagcatc aattgactca ataatttggt tgaagccagt
    12721 atttccgatg tctggtcact tctactgcga ttaaaaaata aagcacttct ggaaatgaag
    12781 tcattcaccg tatggggaac agaaaccatt ttttcaccca ttctcgaaaa atgaaaatta
    12841 gttcgattat tgagaataga gccacacagg gcaataccaa tcgcgccccc aagattacgt
    12901 gttaaattaa atacaccaga ggctaacttc agccgttctt ttggaattcc ccccagcgtt
    12961 aatgttacga tgggggccat cgcaaactgc tgcgaaatcc ctctaattgc ctgtggaaac
    13021 agtaattcct gccatcccca ttcgtgggtt ataggggtaa aaagatacat tgaaaacaca
    13081 aatccgccca gccccgccat caacagccat cgcagattta tttttttact taaccagaag
    13141 taaaagggaa cagaaaataa ctggaaaata cctgttgtgc aaacggcaaa acctatctct
    13201 tcggcattta atccgcgaat ctgccccaaa aacacaggta ttaaatatac agtagagaat
    13261 atcccaaccc ctccagaaaa agaaaaaaaa catcccagag tgaaattttt atccttaaaa
    13321 gcatgaagat ccattatcgg attggatatt gtaagtgtac gcgcagcaaa taaaataaaa
    13381 ctcaccaatg ccagaactga tgtcagcaaa atagtattat catccagcca tccccagcgc
    13441 gctccttctt caagggtata ttccagacat cccagagtca tggccaacag gataatactc
    13501 ggataatcag caactttcag taaagagaga tcaggtttat caaaattaac cagaaaagga
    13561 atactcaaaa ctaaatatat gccaggtaat atattgatat aaaatagcca tcgccagtcg
    13621 aggttatcag tgagccagcc ccccagagtt ggccctagcg tcggtgacag tgaggccagc
    13681 gcactgacta ccgcagcagc caaccccaac tctttaccct ggtaataaat aaacgccatc
    13741 gtgaaaacta aagggatcat ggatgcgcca gcagctccct gtaaagcacg aaacaaaatc
    13801 atgatctgaa tattccatgc aagtccacac gcgattccac acgcgatact cattaaagta
    13861 aatattcccg cggatagagt aaaaagccaa cgagtggaaa atacccgtga cagccaccca
    13921 gagagcggaa taacaataat ctcagcaatt aaatagctgg tctgaagcca ggccatttcg
    13981 tctttacctg cggacagacc accacctatt tcatttaggg atgacgatac aatttgaata
    14041 tcaagctgag caataaataa tcccatacac atactggcga aagcaatatg tttacgcata
    14101 gacatcatct ctcatcaaca ctgacggtga cggacagtcc cgggcgaaga agttgcttta
    14161 attcctttgc gtcgtcaaat gcaatccgta ccgggacacg ctgaacaatt ttggtgaaat
    14221 tgccagtggc attttctatc ggaatcaagc tgaaactggc tcctgtcgct ggggaaaggc
    14281 tttcaatgtg accgtgaaac acttcccctt tgagaatatc agcacggatc tcagccttca
    14341 ttcctggctt cattcccgct atttggtttt ctttataatt tgcatcaacc cacaactcac
    14401 tgactggtac tagtgaaact aacgatgtgc cgccttccac ccaactgcct gtatgggcgc
    14461 tgcggtttgc gacaatgcca tcaatcggcg c
//
LOCUS       DAJTRC010000089        14469 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.089, whole genome shotgun sequence.
ACCESSION   DAJTRC010000089 DAJTRC010000000
VERSION     DAJTRC010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14469)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14469)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14469
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.089"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(11..952)
                     /gene="fabY"
                     /locus_tag="OO357_003069"
                     /gene_synonym="yiiD"
     CDS             complement(11..952)
                     /gene="fabY"
                     /locus_tag="OO357_003069"
                     /gene_synonym="yiiD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001666856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid biosynthesis protein FabY"
                     /protein_id="HCR5887930.1"
                     /translation="MYHLQVPQTEEELERYYHFRWEMLRKPLHQPKGSERDAWDAMAH
                     HQMVVDEQGNLVAVGRLYINADNEASIRFMVVHPDVQDKGLGTLMAMTLESVARQEGV
                     KRVTCSAREDAVEFFAKLGFVNQGEITTPTTTPIRHFLMIKPVATLDDILHRGDWCAQ
                     LQQAWYEHIPLSEKMGVRIQQYTGQKFITTMPETGNQNPHHTLFAGSLFSLATLTGWG
                     LIWLMLRERHLGGTIILADAHIRYSKPISGKPHAVADLGALSGDLDRLARGRKARVQM
                     QVEIFGDETPGAVFEGTYIVLPAKPFGPYEEGGNEEE"
     gene            complement(997..1434)
                     /gene="dtd"
                     /locus_tag="OO357_003070"
     CDS             complement(997..1434)
                     /gene="dtd"
                     /locus_tag="OO357_003070"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000560980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HCR5887931.1"
                     /translation="MIALIQRVTRASVTVEGEVTGEIGAGLLVLLGVEKDDDEQKANR
                     LCERVLGYRIFSDAEGKMNLNVQQAGGSVLVVSQFTLAADTERGMRPSFSKGASPDRA
                     EALYDYFVERCRQQEMNTQTGRFAADMQVSLVNDGPVTFWLQV"
     gene            complement(1431..2303)
                     /locus_tag="OO357_003071"
     CDS             complement(1431..2303)
                     /locus_tag="OO357_003071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor BrkB family protein"
                     /protein_id="HCR5887932.1"
                     /translation="MLKTIQDKARHRTRPLWAWLKLLWQRIDEDNMTTLAGNLAYVSL
                     LSLVPLVAVVFALFAAFPMFSDVSIQLRHFIFANFLPATGDVIQRYIEQFVANSNKMT
                     AVGACGLIVTALLLMYSIDSALNTIWRSKRARPKIYSFAVYWMILTLGPLLAGASLAI
                     SSYLLSLRWASDLNTVIDNVLRIFPLLLSWISFWLLYSIVPTIRVPNRDAIVGAFVAA
                     LLFEAGKKGFALYITMFPSYQLIYGVLAVIPILFVWVYWTWCIVLLGAEITVTLGEYR
                     KLKQAAEQEEDDEP"
     gene            complement(2297..2896)
                     /gene="yihX"
                     /locus_tag="OO357_003072"
     CDS             complement(2297..2896)
                     /gene="yihX"
                     /locus_tag="OO357_003072"
                     /EC_number="3.1.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709685.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphatase"
                     /protein_id="HCR5887933.1"
                     /translation="MLYIFDLGNVIVDIDFNRVLGAWSDLTRIPLASLKKSFHMGEAF
                     HQHERGEISDEAFAEALCHEMALPLSYEQFSHGWQAVFVALRPEVIAIMHKLREQGHR
                     VVVLSNTNRLHTTFWPEEYPEIRDAADHIYLSQDLGMRKPEARIYQHVLQAEGFSPSD
                     TVFFDDNADNIEGANQLGITSILVKDKTTIPDYFAKVLC"
     gene            complement(2995..3780)
                     /locus_tag="OO357_003073"
     CDS             complement(2995..3780)
                     /locus_tag="OO357_003073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5887934.1"
                     /translation="MSLTELTGNPRHDQLLMLIAERGYMNIDELANLLDVSTQTVRRD
                     IRKLSEQGLITRHHGGAGRASSVVNTAFEQREVSQTEKKKAIAEAVADYIPDGSTIFI
                     TIGTTVEHVARALLNHNHLRIITNSLRVAHILYHNPRFEVMVPGGTLRSHNSGIIGPS
                     AASFVADFRADYLVTSVGAIESDGALMEFDVNEANVVKTMMAHARNILLVADHTKYHA
                     SAAVEIGNVAQVTALFTDELPPAALKSRLQDSQIEIILPQEDA"
     gene            complement(3814..4710)
                     /gene="yihV"
                     /locus_tag="OO357_003074"
     CDS             complement(3814..4710)
                     /gene="yihV"
                     /locus_tag="OO357_003074"
                     /EC_number="2.7.1.184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructose kinase"
                     /protein_id="HCR5887935.1"
                     /translation="MIRVACVGITVMDRIYYVEGLPTESGKYVARNYTEVGGGPAATA
                     AVAAARLGAQVDFIGRVGDDDAGNSLLAELESWGVNTRYTKRYNQAKSSQSAIMVDTK
                     GERIIINYPSPDLLPDAEWLEEIDFSQWDVVLADVRWHDGAKKAFTLARQAGVMTVLD
                     GDITPQDISELVALSDHAAFSEPGLARLTGVKEMASALKQAQTLTNGHVYVTQGSAGC
                     DWLENGGRQHQPAFKVDVVDTTGAGDVFHGALAVALATSGDLAESVRFASGVAALKCT
                     RPGGRAGIPDCDQTRSFLSLFV"
     gene            4878..5774
                     /gene="yihU"
                     /locus_tag="OO357_003075"
     CDS             4878..5774
                     /gene="yihU"
                     /locus_tag="OO357_003075"
                     /EC_number="1.1.1.373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfolactaldehyde 3-reductase"
                     /protein_id="HCR5887936.1"
                     /translation="MAAIAFIGLGQMGSPMASNLLQQGHQLRVFDVNAEAVRHLVDKG
                     ATPAANPAQAAKDAEFIITMLPNGDLVRNVLFGENGVCEGLSTDALVIDMSTIHPLQT
                     DKLIADMQAKGFSMMDVPVGRTSANAITGTLLLLAGGTAEQVERATPILMAMGSELIN
                     AGGPGMGIRVKLINNYMSIALNALSAEAAVLCEALNLPFDVAVKVMSGTAAGKGHFTT
                     SWPNKVLSGDLSPAFMIDLAHKDLGIALDVANQLHVPMPLGAASREVYSQARAAGRGR
                     QDWSAILEQVRVSAGMTAKVKM"
     gene            5798..6676
                     /gene="yihT"
                     /locus_tag="OO357_003076"
     CDS             5798..6676
                     /gene="yihT"
                     /locus_tag="OO357_003076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfofructosephosphate aldolase"
                     /protein_id="HCR5887937.1"
                     /translation="MNKYTINDITRASGGFAMLAVDQREAMRMMFAAAGAPAPVADSV
                     LTDFKVNAAKALSPYASAILVDQQFCYRQVVEQNAIAKSCAMIVAADEFIPGNGIPVD
                     SVVIDRKINPLQIKQDGGKALKLLVLWRSDEDAQQRLDMVKEFNELCHSHGLVSIIEP
                     VVRPPRRGDKFDREQAIIDAAKELGDSGADLYKVEMPLYGKGPQQELLCASQRLNDHI
                     NMPWVILSSSVDEKLFPRAVRVAMTAGASGFLAGRAVWASVVGLPDNELMLRDVCAPK
                     LQQLGDIVDEMMAKRR"
     gene            6693..7934
                     /gene="yihS"
                     /locus_tag="OO357_003077"
     CDS             6693..7934
                     /gene="yihS"
                     /locus_tag="OO357_003077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709680.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovose isomerase"
                     /protein_id="HCR5887938.1"
                     /translation="MKWFNTLSHNRWLEQETDRIFDFGKNSVVPTGFGWLGNKGQIKE
                     EMGTHLWITARMLHVYSVAAAMGRPGAYALVDHGIKAMNGALRDKKYGGWYACVNDEG
                     VVDASKQGYQHFFALLGAASAVTTGHPEARKLLDYTIEIIEKYFWSEEEQMCLESWDE
                     AFSKTEEYRGGNANMHAVEAFLIVYDVTHDKKWLDRAIRVASVIIHDVARNNHYRVNE
                     HFDTQWNPLPDYNKDNPAHRFRAFGGTPGHWIEWGRLMLHIHAALEARCEQPPAWLLE
                     DAKGLFNATVRDAWAPDGADGIVYTVDWEGKPVVRERVRWPIVEAMGTVYALYTVTGD
                     RQYETWYQTWWEYCIKYLMDYENGSWWQELDADNKVITKVWDGKQDIYHLLHCLVIPR
                     IPLAPGMAPAVAAGLLDINAK"
     gene            8072..8974
                     /locus_tag="OO357_003078"
     CDS             8072..8974
                     /locus_tag="OO357_003078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose-1-epimerase"
                     /protein_id="HCR5887939.1"
                     /translation="MQITNMHCSGQTVSLAAGDYHATIVTVGAGLAELTFQGCHLVIP
                     HKPEEMPLAHLGKVLIPWPNRIANGCYRYQGQEYQLPINEHSSKAAIHGLLAWRDWQI
                     SELTTTSVTLTAFLPPSYGYPFMLASQVVYSLNAHTGLSVEIASQNIGTVAAPYGVGI
                     HPYLTCNLTSVDEYLFQLPANQVYAVDEHVNPTTLHHVDELDLNFTQAKKIAATKIDH
                     TFKTANDLWEMTITHPQQALSVSLCSDQPWVQVYSGEKLQRQGLAVEPMSCPPNAFNS
                     GIDLLLLEPGKPHRLFFNIYGQRK"
     gene            9173..11209
                     /gene="yihQ"
                     /locus_tag="OO357_003079"
     CDS             9173..11209
                     /gene="yihQ"
                     /locus_tag="OO357_003079"
                     /EC_number="3.2.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfoquinovosidase"
                     /protein_id="HCR5887940.1"
                     /translation="MDTPRPQLLDFQFHQNNDSFTLHFQQRLILTHNKDNPCLWIGSG
                     IADIDMFRGNFSIKDKLQEKIALTDAIVSQSPDGWLIHFSRGSDISATLNISADDQGR
                     LLLELQNDNLNHNRIWLRLAAQPEDHIYGCGEQFSYFDLRGKPFPLWTSEQGVGRNKQ
                     TYVTWQADCKENAGGDYYWTFFPQPTFVSTQKYYCHVDNSCYMNFDFSAPEYHELALW
                     EDKATLRFECADTYISLLEKLTALLGRQPELPDWIYDGVTLGIQGGTEVCQKKLDTMR
                     NAGVKVNGIWAQDWSGIRITSFGKRVMWNWKWNSENYPQLDSRIKQWYQEGVQFLAYI
                     NPYVASDKDLCEEAAQHGYLAKDASGGDYLVEFGEFYGGVVDLTNPEAYAWFKEVIKK
                     NMIELGCGGWMADFGEYLPTDTYLHNGVSAEIMHNAWPALWAKCNYEALEETGKLGEI
                     LFFMRAGSTGSQKYSTMMWAGDQNVDWSLDDGLASVVPAALSLAMTGHGLHHSDIGGY
                     TTLFEMKRSKELLLRWCDFSAFTPMMRTHEGNRPGDNWQFDGDAETIAHFARMTTVFT
                     TLKPYLKEAVALNAKSGLPVMRPLFLHYEDDAHTYSLKYQYLLGRDILVAPVHEEGRS
                     DWTLYLPEDNWVHAWTGEAFRGGEVTVNAPIGKPPVFYRADSEWAALFASLNSI"
     gene            11255..12629
                     /locus_tag="OO357_003080"
                     /pseudo
     CDS             11255..12629
                     /locus_tag="OO357_003080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312826.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
     gene            12672..14075
                     /locus_tag="OO357_003081"
     CDS             12672..14075
                     /locus_tag="OO357_003081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5887941.1"
                     /translation="MSDHNPLTLKLNLREKIAYGMGDVGSNLMLCIGTLYLLKLYTDE
                     LGMPAYYGGIIFLVAKFFTAFTDMLTGFLLDSRKNIGPKGKFRPFILYAAVPAALIAT
                     LQFIATTFCLPVKTTIATTLFMMFGLSYSLMNCSYGAMIPAITKNPNERAQLAAYRQG
                     GATIGLLICTVAFIPLQSLFSDSTVGYACAALMFSIGGFIFMMLCYRGVKEHYVDTAP
                     TGHKASILKSFCAIFRNPPLLVLCIANLCTLAAFNIKLAIQVYYTQYVLNDINLLSWM
                     GFFSMGCILVGVLLVPVTVKCFGKKQVYLAGMVLWAVGDILNYFWGSNSFTFVMFSCV
                     AFFGTAFVNSLNWALVPDTVDYGEWKTGIRAEGSVYTGYTFFRKISAALAGFLPGIML
                     TQIGYVPNIAQSDATLQGLRQLIFIWPCALAIIAALTMGFFYTLNEKRFALIIEEINQ
                     RKNKEMATEKKTASVTL"
     gene            14143..>14469
                     /gene="ompL"
                     /locus_tag="OO357_003082"
     CDS             14143..>14469
                     /gene="ompL"
                     /locus_tag="OO357_003082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418311.1"
                     /note="porin involved in the transport of small molecular
                     weight solutes (up to 600 Daltons) across the cell wall;
                     specific substrate still unknown; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpL"
                     /protein_id="HCR5887942.1"
                     /translation="MKKINAIILLSSLTSASVFAGAYVENREAYNLASDQGEVMLRVG
                     YNFDMGAGIMLTNTYTFQREDELKHGYNEIEGWYPLFKPTDKLTIQPGGLINDKSISS
                     GGAVYLD"
BASE COUNT         3491 a         3769 c         3648 g         3561 t
ORIGIN      
        1 tctccataag ctactcttct tcgttcccgc cctcttcata cgggccaaat ggcttcgcgg
       61 gcagaacgat atacgtgcct tcaaacactg cacccggcgt ctcgtcgcca aagatttcga
      121 cctgcatctg cacccgtgct tttcgtccgc gcgccagacg gtcgagatcg ccgcttaagg
      181 caccaaggtc ggctaccgca tggggtttac cgctaatcgg tttgctgtag cggatatgcg
      241 catccgccag aataatcgtt ccgccgaggt ggcgttcacg cagcatcagc cagataagcc
      301 cccaaccggt gagcgtcgcc agcgagaata aactcccggc aaacagcgta tggtgcggat
      361 tctgattgcc ggtttccggc atggtggtga taaatttttg cccggtatat tgttgaatac
      421 gcacgcccat tttttcacta agcgggatat gttcgtacca cgcctgttgc agctgcgcgc
      481 accagtcgcc gcgatgcaaa atatcatcca gagtggcgac gggcttaatc atcaaaaaat
      541 ggcgaatcgg cgtggtggtt ggcgtggtga tttctccctg attaacaaac cccagcttgg
      601 cgaaaaactc caccgcgtct tcacgggcgc tacaggtcac gcgcttaacg ccttcctgac
      661 gcgccaccga ctccagggtc atcgccatca gcgttcctaa ccctttgtcc tgcacgtccg
      721 ggtgaacgac cataaagcga atggacgctt cattgtcggc attaatatac aatcggccta
      781 ccgccaccag attaccctgc tcgtcgacga ccatctggtg atgcgccatc gcatcccacg
      841 cgtcgcgttc cgaacctttt ggttgatgca ggggcttacg caacatttcc cagcgaaagt
      901 gatagtaacg ctctaactct tcttctgttt gtggaacctg aaggtgatac atagcgatac
      961 tctctcttgt ttcccgtgac aaccctggaa gctggctcat acctgcaacc agaatgtcac
     1021 ggggccatca ttgaccagcg atacctgcat atcggcagcg aagcgtcctg tttgcgtgtt
     1081 catctcttgc tggcggcagc gttcgacgaa atagtcatat aacgcctctg cgcgatccgg
     1141 tgatgcgcct ttggagaaac ttgggcgcat cccccgttcg gtatctgcgg cgagggtaaa
     1201 ctgggaaacc accagtacac tgccgcccgc ctgttgcacg ttgagattca tcttgccttc
     1261 ggcatcgcta aagatgcggt agccgagcac acgctcgcac agacggtttg ctttctgttc
     1321 gtcgtcatcc ttttcgacac ccaataacac caaaagtccc gcgccaattt cgcccgtcac
     1381 ttctccctcc acggtgacgc tggcacgggt tacgcgttga attaatgcaa tcatggttcg
     1441 tcgtcttctt cttgttcagc tgcttgtttt agtttgcggt attccccgag agtgacagta
     1501 atttccgcgc caagcaagac gatacaccat gtccagtaga cccaaacaaa gagaatgggg
     1561 atcaccgcca gcacaccgta aatgagctga tatgacggga acatggtgat ataaagcgcg
     1621 aaacctttct ttcctgcttc gaacaggagt gcggcgacaa acgcgccgac aatcgcgtcg
     1681 cggttaggta cgcggatggt aggaacaatg ctgtacagca accagaagga gatccacgac
     1741 aacagcaacg gaaaaatacg cagcacgttg tcgatgacag tattgagatc gctcgcccag
     1801 cgcagggaga gcaaatagga actgatcgcc agactggccc ctgccagcag cggccccagc
     1861 gttaaaatca tccagtacac ggcgaacgag taaatttttg gtcgcgctcg tttactgcgc
     1921 cagatggtat tcaacgcgct atcgatggag tacatcaaca ataacgcggt gacgatcagc
     1981 ccgcacgccc caacggcggt catcttgttg gaattagcaa caaattgttc gatataccgc
     2041 tgaataacat cgccagtagc aggcagaaag ttggcaaaaa taaagtgacg taactgaatg
     2101 ctgacgtcgg aaaacatggg aaaagcggcg aaaagcgcaa aaacaacggc aaccagcggc
     2161 actaatgaga gcaacgacac ataggcaagg ttacctgcca gggttgtcat gttgtcctca
     2221 tcaatacgtt gccagagtag ttttagccag gcccatagtg gacgggtacg gtgcctggct
     2281 ttgtcctgaa tggtttttag cataacacct tcgcgaaata gtccgggatg gtggttttat
     2341 ctttcaccag aatactggta atgcctagct gattagctcc ttctatatta tcggcgttat
     2401 cgtcgaaaaa gaccgtatcg ctgggtgaaa aaccttccgc ctgcaaaaca tgctggtaaa
     2461 ttcgtgcttc aggtttgcgc atccccagat cttgcgacag atagatatgg tcagcagcat
     2521 cacgaatttc cgggtattct tccggccaga aggtggtatg caggcggttg gtattggaaa
     2581 gcaccaccac gcgatgcccc tgctcacgca gtttatgcat gatggcgatc acttccgggc
     2641 gcagcgcaac aaacaccgcc tgccagccgt gagagaactg ctcgtagctt agcggtagag
     2701 ccatctcatg acacagcgcc tctgcgaacg cttcgtcgct aatttcccca cgctcatgct
     2761 gatgaaacgc ctcccccata tgaaaactct tcttaagcga tgccagcgga atacgcgtta
     2821 aatcgctcca ggctcccagc acacggttaa agtcgatatc gacaatcaca ttacctaaat
     2881 caaagatata gagcattttt gcctcctttc gcgccacaag aaattaactg tagcgggaaa
     2941 ggatgggttt ggctatgtgg caaggtatga agccaggtta acggtcacaa aaatctacgc
     3001 gtcttcctgg ggaagaatga tttcaatttg gctgtcttgt aagcgtgatt ttagcgcagc
     3061 gggcggcagc tcgtcggtaa agagcgcagt gacctgtgcc acgttaccaa tttcaaccgc
     3121 cgccgaagca tgatacttag tgtgatcggc gaccagcaga atatttctcg cgtgcgccat
     3181 catcgttttc accacgttag cttcgtttac atcaaactcc atcaacgcgc catcgctctc
     3241 aatcgcccca acgcttgtta ccagataatc agcacgaaaa tcagccacaa aggacgccgc
     3301 tgaagggcca atgatcccgc tattatgaga gcgcaacgta ccgccgggca ccatcacttc
     3361 aaagcgcggg ttgtggtaaa gaatatgcgc cacacgcagg ctgttggtga ttatccgcaa
     3421 atgattatgg ttaagtaacg cccgggcaac atgctcaaca gtcgtaccaa tggtgataaa
     3481 tattgttgaa ccatcaggaa tatagtctgc caccgcttcg gcaatcgctt tttttttctc
     3541 ggtttgcgaa acctcacgct gctcgaacgc cgtattaacg acgctggaag cccgacccgc
     3601 gccaccgtga tggcgcgtaa tcaggccttg ctcgcttaat ttacgaatat cccggcggac
     3661 cgtctgcgtg gagacatcca gcagatttgc cagctcatca atattcatat acccacgctc
     3721 ggcgatcagc atgaggagtt ggtcgtgccg cgggttaccg gttagttcgg taaggctcat
     3781 gaaaattcct cgaaaaacca tcaccctggc attttataca aaaagtgaca aaaaagatcg
     3841 ggtttgatca cagtcaggga tcccggctcg tccaccggga cgtgtgcatt ttaacgccgc
     3901 tacaccgctg gcgaagcgga ctgactccgc taaatcccca cttgttgcca gcgccaccgc
     3961 caaagcgccg tgaaaaacat ctcccgcacc tgtggtatct accacatcaa ctttgaaggc
     4021 cggttgatgc tgacgcccac cattttccag ccagtcgcag cctgcgctac cctgggtcac
     4081 atagacatga ccatttgtga gcgtttgtgc ctgttttagc gcactggcca tctctttcac
     4141 gcccgttaag cgcgccagac ccggttctga aaaggccgcg tgatcgctta atgccaccag
     4201 ctcactgata tcctgcggcg taatgtcccc gtccagaacg gtcatcacac ccgcctgacg
     4261 ggccagggtg aaggcttttt tagcgccgtc gtgccagcgt acatctgcca gcacaacatc
     4321 ccactgagag aaatcaattt cctccaacca ctctgcgtca ggcagcagat ccgggctggg
     4381 gtagttaatg attatccgct cgcctttggt atccaccatg atggcggatt gcgaagattt
     4441 cgcctggtta taccgtttgg tgtaacgggt gttaacgccc caggattcca gctctgccag
     4501 caggctgttg cccgcgtcgt catcacctac gcgaccaata aaatcgacct gcgcccccag
     4561 ccttgccgcc gcaaccgctg ccgtcgccgc tggcccgcca ccaacttccg tataatttct
     4621 cgccacgtat ttaccgctct ccgtcggtaa cccttccacg taatagatgc gatccatcac
     4681 ggttatacct acacaagcaa cacgaatcat ggtcattcct taaacatttt gggatgcgtt
     4741 cattttaatt tcaccaattg tttaaaaagt gacggctgtc aattttttga cgataaatta
     4801 caaattcgat caaaaacaga caagcaaagc atgtcataaa tgacaaaaag tgacatcact
     4861 gtattcagga gagggttatg gcagcaatcg cgtttatcgg tttaggacaa atgggttcgc
     4921 caatggcgag caatttattg cagcaagggc atcaacttcg cgtctttgat gtgaacgccg
     4981 aagctgtgcg gcatctggta gacaaaggtg cgactcccgc cgccaacccg gcgcaggccg
     5041 ctaaagatgc cgaatttatc attaccatgt tgccgaatgg cgatctggtg cgcaacgtgt
     5101 tgttcggtga aaacggcgtt tgcgaaggct tatctaccga tgcgctggtc attgatatgt
     5161 ccaccatcca tccgctgcaa accgataaat tgattgccga tatgcaagcc aaaggcttca
     5221 gcatgatgga tgttccggta ggccgtactt ctgcaaatgc cattaccggt actctgttac
     5281 tgctggctgg cggcaccgct gaacaagttg aacgtgccac accgatcctg atggcgatgg
     5341 gcagtgagtt gatcaacgct ggcggtccgg gcatggggat ccgcgttaag ctcatcaaca
     5401 actacatgag catcgcgctc aatgcgcttt cggcagaagc cgccgttttg tgcgaagccc
     5461 tgaatcttcc cttcgatgtt gccgtcaaag tgatgagcgg taccgccgcc ggtaaaggtc
     5521 acttcaccac ttcctggcca aacaaagtcc tcagcggaga tctttctccc gccttcatga
     5581 tcgatcttgc ccataaggat cttggcatcg cccttgatgt cgccaaccag ctgcatgtgc
     5641 caatgccgct gggggccgcc tcacgggagg tttatagcca ggcgcgcgca gcgggtcgcg
     5701 gtcgccagga ctggtccgcc attctggaac aggtccgtgt cagtgccggg atgactgcca
     5761 aagtaaaaat gtaacgactg gataaaggaa taaatgaatg aataagtaca ccatcaacga
     5821 cattacgcgc gcatcgggcg gttttgccat gctggcggtc gatcagcgcg aagccatgcg
     5881 catgatgttt gccgcggctg gggcacccgc tccggtagcc gatagcgttt taactgattt
     5941 caaagttaac gctgcaaagg ccctctcgcc ttatgcctcg gcgattctgg tagatcaaca
     6001 attctgctac cgccaggtgg ttgagcaaaa cgcgattgcc aaaagttgcg ccatgattgt
     6061 cgccgccgat gagttcattc ctggcaacgg tattccggtc gatagcgtgg ttattgaccg
     6121 caaaatcaat ccgctacaga tcaaacaaga cggtggcaaa gccttaaaac tgctggtgct
     6181 gtggcgtagt gatgaagatg cgcagcaacg tctggatatg gtgaaagagt tcaacgaact
     6241 gtgccactca cacggtctgg taagcatcat tgagccagtc gtccgcccac cgcgtcgtgg
     6301 cgataaattc gatcgcgaac aagcgatcat cgatgccgcc aaagagctgg gcgacagtgg
     6361 cgctgacctc tacaaagttg aaatgccgct ttatggcaaa ggtccgcaac aagagcttct
     6421 ctgtgcttca caacgtctga atgaccatat caatatgcca tgggtgatcc tctcttccag
     6481 tgtcgacgaa aaactgttcc cgcgtgccgt acgcgtggca atgacggcgg gcgcatcggg
     6541 attcctggca ggccgtgcag tctgggcatc ggtcgtcggt ttaccagaca acgagctgat
     6601 gctccgtgac gtttgcgcac cgaaattaca acaacttggc gatatcgtcg acgaaatgat
     6661 ggctaaacgc cgctaagatg aggataaaag gaatgaaatg gtttaacacc ctaagccaca
     6721 accgttggct ggaacaggaa accgaccgca tctttgattt tggtaaaaat tccgtagtgc
     6781 cgactggttt tggctggtta ggcaataaag ggcaaatcaa agaagagatg ggcacccatc
     6841 tgtggatcac agctcgtatg ttgcacgttt attccgttgc tgcggcgatg ggtcgacctg
     6901 gcgcttacgc gttggttgat cacggtatta aagccatgaa cggcgcactg cgtgataaaa
     6961 aatatggcgg ctggtatgcc tgcgtgaatg acgagggcgt ggtggatgcc tccaaacagg
     7021 gctatcaaca cttctttgct ctgctgggtg ccgccagcgc cgtcacaacg ggtcacccgg
     7081 aagcgcgcaa gctgctcgat tacaccattg agattatcga gaaatatttc tggagcgaag
     7141 aagagcagat gtgcctggag tcctgggacg aagccttcag caaaaccgaa gagtaccgcg
     7201 gcggcaatgc caatatgcac gcggtggaag ctttcttgat tgtttatgac gtcactcacg
     7261 acaaaaaatg gctggatcgc gcgattcgcg tggcttccgt gattatccac gacgtcgcca
     7321 gaaataatca ttatcgcgtt aacgaacact tcgataccca gtggaatccg ctgccggatt
     7381 acaacaaaga taacccggcg catcgcttcc gcgcgttcgg aggtacacca ggccactgga
     7441 tcgaatgggg ccgtttaatg ctgcacatcc atgctgccct ggaagcccgt tgcgaacaac
     7501 caccagcatg gctgctagaa gatgccaaag gtctgtttaa cgccaccgtg cgcgatgcct
     7561 gggcacccga tggagcggac gggattgttt ataccgttga ctgggaagga aaaccggtgg
     7621 tccgcgaacg tgtacgttgg cctatcgtcg aagcaatggg tacggtctac gcgctctaca
     7681 ccgtcactgg cgatcgccag tatgaaacct ggtatcaaac atggtgggag tactgcatta
     7741 agtacctgat ggactatgaa aatggttcct ggtggcagga gctggatgcg gacaataagg
     7801 tcatcaccaa agtctgggac ggcaaacagg atatttatca cctgctgcat tgcctggtga
     7861 tcccgcgtat cccgttagcg cccggcatgg ctccagcggt tgcagcgggt ctgctggata
     7921 ttaatgcgaa ataactgatc tgaaaatagg gtgcgggttt tcccctcacc ctaaccctct
     7981 ccccaaaggg gcgaggggac cgtccggtgc agtttttggg tttgacatca gcctcacggg
     8041 aggctgtatg tcgttaatta aggtgccttg tatgcaaatc accaatatgc attgcagtgg
     8101 acagaccgtt tcacttgccg cgggggatta tcacgcgacc attgtgaccg tcggtgctgg
     8161 tctggctgaa ttaacctttc agggttgtca tttagtcatc ccgcacaaac ccgaagagat
     8221 gccgctggcg catctgggta aagtgcttat tccgtggccg aaccggatcg ccaacggctg
     8281 ttaccgttat caggggcagg aatatcagtt acccattaat gaacatagtt caaaagccgc
     8341 tattcacggg ctgcttgcct ggcgagactg gcaaattagt gaattaacca ccaccagcgt
     8401 taccctgaca gcttttctgc cgcccagtta cggctatccg tttatgctgg cttcacaagt
     8461 tgtttattcg ctcaacgcac acacgggtct ttccgttgag atcgcaagcc agaatatcgg
     8521 cactgtcgca gcgccttatg gcgtgggtat tcatccttat ttaacctgca acttaacatc
     8581 tgttgatgaa tatctgttcc agcttccggc aaaccaggtt tatgccgtcg atgaacacgt
     8641 aaatccgaca acgcttcacc acgttgatga gctggattta aatttcacgc aggcgaaaaa
     8701 aatagccgcg acaaaaatcg accacacctt taaaaccgct aatgatttgt gggaaatgac
     8761 cattacccat ccacagcagg cgctttcggt aagcctttgt tccgatcaac cctgggtgca
     8821 ggtttatagc ggtgaaaaat tacagcgtca gggactggca gttgaaccta tgagttgccc
     8881 gcctaacgcc tttaattcag gtatcgactt attattactg gaaccaggaa aaccgcaccg
     8941 cctgtttttt aatatttacg gacagagaaa ataacttgta ttgattagca gtcaaaatca
     9001 ttgaagaaca tttcactcgc taaaggcaac attatgtatc ttcaatttat taagttaaaa
     9061 acagctcagc ttctgtaaag tttttatata tcagttttat gagaatcatt ttacataagc
     9121 ctgtaataac aggaaggcaa caatctcgtt ctcaactcta tggagattag ttatggatac
     9181 gccacgtcca cagttattag attttcaatt tcatcagaat aacgacagtt ttaccctaca
     9241 ttttcaacaa cgtcttattt taacccataa caaagataat ccttgtttat ggattggctc
     9301 aggtatagcg gatatcgata tgttccgcgg taatttcagc attaaagata aactacagga
     9361 gaaaattgcg cttaccgacg ccatcgtcag ccagtcaccg gatggttggt taattcattt
     9421 cagccgtggt tctgacatta gcgccacgct gaatatctct gccgacgatc agggccgttt
     9481 attgctggaa ctacaaaacg acaaccttaa ccacaaccgt atctggctgc gccttgccgc
     9541 tcaaccagag gaccatatct acggctgcgg cgaacagttt tcctacttcg atctgcgcgg
     9601 caaaccgttc ccgctatgga ccagtgaaca aggcgttggt cgcaacaaac aaacctatgt
     9661 cacctggcag gccgactgca aagaaaatgc gggcggcgac tattactgga ctttcttccc
     9721 acagcctacg tttgtcagca cgcagaagta ttactgccat gttgataaca gttgctacat
     9781 gaacttcgac tttagcgccc cggaatacca tgaactggcg ctgtgggaag acaaagctac
     9841 gctacgtttt gaatgtgctg acacatacat ctccctgctg gaaaaattaa ctgccctgct
     9901 gggacgccag ccagaactgc ccgactggat ttatgacggg gtaacgctcg gcattcaggg
     9961 cgggacggaa gtgtgccaga agaaactgga caccatgcgt aacgcgggcg tgaaggttaa
    10021 cggcatctgg gcgcaggact ggtctgggat ccgtataacc tcttttggca agcgcgtgat
    10081 gtggaactgg aagtggaaca gcgaaaacta cccgcaactg gattcacgca ttaagcagtg
    10141 gtatcaggag ggcgtgcagt tcctggccta tatcaacccg tatgttgcca gcgataaaga
    10201 tctctgcgaa gaagcggcac aacacggcta tctagcaaaa gatgcctctg gcggtgacta
    10261 tctggtggag tttggcgagt tttacggcgg cgttgtcgat ctcactaatc cagaagccta
    10321 cgcctggttc aaggaagtga tcaaaaagaa catgattgaa ctcggctgcg gcggctggat
    10381 ggctgacttc ggcgagtatc tgcccaccga cacgtacctg cataacggcg tcagtgccga
    10441 aattatgcat aacgcctggc ctgcgctgtg ggcgaagtgt aactacgaag cccttgaaga
    10501 aacgggcaag ctcggcgaga tcctcttctt tatgcgcgcc ggttctaccg gtagccagaa
    10561 atactccacc atgatgtggg cgggcgacca gaacgtggac tggagtctcg acgatggcct
    10621 ggcgtccgta gtcccggcgg cgctgtcgct ggcaatgacc ggacatggtc tgcatcacag
    10681 cgacattggc ggttacacca ccctgtttga gatgaagcgc agcaaagagc tgctgctgcg
    10741 ctggtgcgat ttcagcgcct tcacgccgat gatgcgcacc cacgaaggta accgtcctgg
    10801 cgacaactgg cagtttgacg gcgatgcgga aactatcgcc cattttgccc gtatgactac
    10861 cgttttcacc accctgaaac cttatctgaa agaggccgtc gcgctgaatg cgaagtccgg
    10921 tctgccggtc atgcgcccgc tgttcctgca ttacgaagac gatgcacata cctattccct
    10981 gaaatatcag tacctgttag gccgcgacat tctggtcgct ccggtacacg aagaaggtcg
    11041 cagcgactgg acgctctatc tgccggagga taactgggta catgcctgga cgggtgaagc
    11101 gttccggggc ggggaagtta ccgttaatgc gcccatcggc aagccgccgg tcttttatcg
    11161 cgccgatagc gaatgggcgg cactgttcgc gtcgttaaac agcatctaat cacatccgcc
    11221 cgtggggaaa ctcatgggca aagggagaac atcgatgagt cacatcacaa cggaagatcc
    11281 agcaacttta cgcctgccct ttaaagagaa actctcttac ggtattggcg acctggcctc
    11341 taacatcctg ctggatatcg gtacgcttta tcttttgaag ttttataccg acgttctggg
    11401 gctgccaggc acctatggcg gcattatctt tttgatttca aaattcttta ctgcgtttac
    11461 cgatatgggt accggcatta tgttggattc ccgacgcaag atcggtccaa aaggtaagtt
    11521 ccgtcctttt attctgtatg cgtcattccc ggtcacctta ctggcgatcg ccaactttgt
    11581 cggcacaccg tttgatgtca ccggtaaaac ggtgatggcc actattctgt ttatgcttta
    11641 cggactgttt ttcagcatga tgaactgctc ctacggcgca atggtcccgg caatcaccaa
    11701 aaaccccaac gaacgcgcct cactggcggc atggcgtcag ggcggcgcta cgctgggcct
    11761 gctgctgtgc acggtgggat tcgtgccagt tatgaatctt atcgaaggta atcagaaact
    11821 tggctatatc ttcgccgcca cgctgttttc actgttcggc ctgctgttta tgtggatctg
    11881 ctactcaggc gtgaaagagc gttatgtcga aacccaacca accaatccgg cgcaaaagcc
    11941 tggcctgttg caatctttcc gcgcaattgc cggtaaccgc ccactgttca ttctgtgtat
    12001 tgccaacctc tgcaccttag gggcgtttaa cgtcaagctc gccattcagg tctattacac
    12061 ccagtacgta cttaacgatc ccatcctgtt gtcgtatatg ggatttttca gcatgggctg
    12121 tattttcatc ggcgtgttcc tgatgcctgg cgcagtcaga cgttttggta agaagaaggt
    12181 ctatatcggc ggcctgctga tttgggtgct gggcgatctg ctcaactatt tcttcggcgg
    12241 cggctcggtc agctttgtgg cgttctcctg cctggcgttc ttcggctcag cgcctgaact
    12301 gggcgctggt ttccgacacc gtcgagtacg gcgagtggcg caccggcgtg cgttcggaag
    12361 gcacggtcta caccggcttc accttctttc gcaaagtgtc tcaggcgctg gctggtttct
    12421 tccccggctg gatgctgacg caaattggct atgtgccgaa cgtcgcacag gctgaccaca
    12481 ccattgaagg gttacgccag ttgatcttca tctacccaag cgcactggcg gtagtcacca
    12541 ttgtggcgat gggttgcttc tacagcctga acgagaagat gtatgtccgc attgtggaag
    12601 agatagaagc ccgtaaacgc acggcgtaat aataataaac gacgccctgc ggggcgttat
    12661 aaggagtgat tatgtctgac cataatccac tgacattaaa actgaatctg cgggaaaaaa
    12721 tcgcctatgg tatgggcgac gtcggttcga atttaatgct ctgcatcggt actctgtatc
    12781 tcctcaaact ttataccgat gaattaggga tgcctgctta ctatggcggg attatctttc
    12841 tggtcgcgaa gttttttacc gcgtttaccg atatgctcac cggattttta ctcgactcgc
    12901 ggaaaaatat tgggcctaaa ggcaaatttc gtccctttat tttatatgca gcagttccgg
    12961 cggcgttaat tgcaacgctc cagtttatcg ccaccacctt ttgtttgccg gttaaaacga
    13021 cgattgccac cacgctgttt atgatgtttg gactttcata cagtctgatg aactgctcgt
    13081 atggtgcgat gatcccggca attaccaaaa acccgaatga gcgcgcgcag ctcgcggctt
    13141 accgccaggg tggtgcaacc atagggctat tgatttgtac cgtggcgttt attccgttgc
    13201 agtcgctttt ttctgactca accgtcggtt atgcctgtgc ggcacttatg ttctccattg
    13261 gcggctttat ttttatgatg ctgtgctaca gaggcgtcaa agagcattat gtggacacag
    13321 cgccaaccgg acataaagcc agtattctca aatctttttg cgcgatattt cgtaatccac
    13381 cattgctggt tttatgcatt gctaacctgt gtactctggc ggcatttaat atcaaactgg
    13441 cgattcaggt ctattacacc cagtatgtgc tgaatgatat taatttattg tcgtggatgg
    13501 gattcttcag tatgggatgc atcctcgtcg gcgtattact ggtgccagta actgtaaaat
    13561 gttttggtaa aaaacaggtt tatttagctg gcatggtgct atgggcggtg ggtgatatac
    13621 tgaattattt ctggggaagt aactctttca ctttcgtcat gttctcttgt gtcgcctttt
    13681 ttggcacggc ctttgtaaac agcctgaact gggcactggt accagatacc gtagattacg
    13741 gtgaatggaa aaccggtatt cgtgccgaag gttctgttta taccggttat accttctttc
    13801 gtaaaatttc tgccgcactt gctggcttct tgccaggcat tatgctgacg caaattggtt
    13861 atgttcccaa catcgcgcaa agcgatgcga cattacaggg tttgcgtcag ctcattttta
    13921 tctggccttg tgcactggca attattgctg cattgacgat gggattcttt tacacactca
    13981 atgaaaaacg gtttgcattg attattgagg aaatcaacca acgcaaaaat aaagagatgg
    14041 cgacagaaaa aaaaacggct tccgtaacgt tataaatgtc aatagccgct atttccatcc
    14101 tggtgggtgg cggcctccct acgtttaaaa aatggactta ttatgaaaaa gattaatgca
    14161 ataattttat tatcttctct gacctctgcc tcggtatttg ctggcgcata cgtcgaaaac
    14221 agggaggcat ataatcttgc ctccgatcag ggcgaggtta tgctacgtgt gggttataac
    14281 ttcgatatgg gcgcgggtat tatgttaacc aatacctaca cctttcagcg agaagatgaa
    14341 ctaaaacatg gctataatga aattgaaggt tggtatcctt tattcaaacc aaccgataaa
    14401 ttaaccatcc agcccggtgg cttaattaat gataagagta tcagttcggg tggtgcagtg
    14461 tatctggac
//
LOCUS       DAJTRC010000090        14306 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.090, whole genome shotgun sequence.
ACCESSION   DAJTRC010000090 DAJTRC010000000
VERSION     DAJTRC010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14306)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14306)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14306
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.090"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            1..3051
                     /locus_tag="OO357_003083"
                     /pseudo
     CDS             1..3051
                     /locus_tag="OO357_003083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001215568.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            3061..6707
                     /locus_tag="OO357_003084"
                     /pseudo
     CDS             3061..6707
                     /locus_tag="OO357_003084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416621.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4132 domain-containing protein"
     gene            6768..7175
                     /locus_tag="OO357_003085"
     CDS             6768..7175
                     /locus_tag="OO357_003085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887943.1"
                     /translation="MIVQKELVAIYDYEVPVPEDPFSFRLEIHKCSELFTGSVYRLER
                     FRLRPTFHQRDREDVDPLINDALIYIRDEFIDEQKLRGESPEPVIVIFNCKLQNIFNQ
                     QIELYTLDNSSRSTCNLKYDEYSFIKGGNGIPP"
     gene            7717..8805
                     /locus_tag="OO357_003086"
     CDS             7717..8805
                     /locus_tag="OO357_003086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310954.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HCR5887944.1"
                     /translation="MSPQNNHLQRPPAAVLYADELAKLKQNDNAPCPPGWQLSLPAAR
                     AFILGDSAQNISRKVVISPSAVERMLVTLATGRGLMLVGEPGTAKSLLSELLATSISG
                     DAGLTIQGGASTTEDQIKYGWNYALLINHGPSTEALVPAPLYQGMRDGKIVRFEEITR
                     TPLEVQDCLLGMLSDRVMTVPELTGEASQLYAREGFNIIATANTRDRGVNEMSAALKR
                     RFDFETVFPIMDFAQELELVASASARLLAHSGIPHKVPDAVLELLVRTFRDLRANGEK
                     KTSMDTLTAIMSTAEAVNVAHAVGVRAWFLANRAGEPADLVDCIAGTIVKDNEEDRAR
                     LRRYFEQRVATHKEAHWQAYYQARHRLP"
     gene            8816..11095
                     /locus_tag="OO357_003087"
     CDS             8816..11095
                     /locus_tag="OO357_003087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708008.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887945.1"
                     /translation="MSEPLIVGIRHHSPACARLVKSLIESQRPRYVLIEGPADFNDRV
                     DELFLAHQLPVAIYSYCQYQDGAAPGRGAWTPFAEFSPEWQALQADRRIQAQTYFIDL
                     PCWAQSEEEDDSPDTQEESQTLLLRATRMDNSDTLWDHLFEDESQQTALPSALAHYFA
                     QLRGDSPGDALNRQREAFMARWIGWAMQQNNGDVLVVCGGWHAPALAKMWRECPQVIN
                     TPELPSLADAVTGCYLTPYSEKRLDVLAGYLSGMPAPVWQNWCWQWGLQQAGEQLLKT
                     VLTRLRQHKLPASTADMAAAHLHAMALAQLRGHTLPLRTDWLDAIAGSLIKEALNAPL
                     PWSYRGVIHPDTDPILLTLIDTLAGDGFGKLAPSTPQPPLPKDVTCELERTAISLPAE
                     LTLNRFNPNGLAQSQVLHRLAILEIPGIVRQQGSTLTLAGNGEERWKLTRPLSQHAAL
                     IEAACFGATLLEAARHKLEADMLDAGGIGSITTCLSQAASAGLASFSQQLLEQLTLLI
                     AQENQFAEMGQALEVLYALWRLDEISGMQGAQILQTTLCAAIDRTLWLCESNGRPDEK
                     EFHAHLHSWQALCHILRDLHSGVQLPGVSLSAAVALLERHSQAIHVLALDRGATLGAL
                     MRLEHPNASAEAALTMLAQLSPAQSGEALHGLLALARHQLACQPAFIAGFSSHLNQLS
                     DADFTNALPDLRAAMAWLPPRERGTLAHQVLEHYQLAQLPVSALQMPLHCPPQAIAHH
                     QQLEQQALASLQHWGVFHV"
     gene            11088..12224
                     /locus_tag="OO357_003088"
     CDS             11088..12224
                     /locus_tag="OO357_003088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708009.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HCR5887946.1"
                     /translation="MSELNDLLTTRELQRWRLILGEAAETTLCGLDDNARQIDHALEW
                     LYGRDPERLQRGERSSGLGGSNLTTPEWINSIHTLFPQQVIERLESDAVLRYGIEDVV
                     TNLDVLERMQPSESLLRAVLHTKHLMNPEVLAAARRIVRQVVEEIMARLAKEVRQAFS
                     GVRDRRRRSFIPLARNFDFKSTLRANLQHWHPQHGKLYIEFPRFNSRIKRQSEQWQLV
                     LLVDQSGSMVDSVIHSAVMAACLWQLPGIRTHLVAFDTSVVDLTADVADPVELLMKVQ
                     LGGGTNIASAMEYGRQLIEQPAKSVIILVSDFYEGGSSSLLTHQVKKCVQSGIKVLGL
                     AALDSTATPCYDRDTAQALVNVGAQIAAMTPGELASWLAENLQS"
     gene            12221..14224
                     /locus_tag="OO357_003089"
     CDS             12221..14224
                     /locus_tag="OO357_003089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887947.1"
                     /translation="MNSLRPELLELTPQALTALSNAGFVKRSLKELENGNVPEISHEN
                     SALIATFSDGVRTQLANGQALKEAQCTYGASGMCRHRVMLVLSYQRLCATAQPTGKEE
                     EWDPAIWLEELATLPDATRKRAQALVAKSITIELFCAPGEIPSARLPMSDVRFYSRSS
                     IRFARCDCIEGTLCEHVVLAVQAFVQAKAQQAELTHLIWQMRSEHVTSSDDPFASEEG
                     KTCRQYVQQLSQALWLSGISQPLIHYEAAFSRAQQAAERCSWRWVSESLRQLRASVDA
                     FHARASHYHAGECLRQLAALNSRLNCAQEMARSDSVGEVPPVPWRTVVGSGIAGEAKL
                     DHLRLVSLGMRCWQDIEHYGLRIWFTDPDTGSILHLSRSWPRSEQEDSPAATRRLFSF
                     QAGALAGGQIVSQAAKRSADGELLLATRNRLSSVVPLSPDAWQMLSAPLRQPGIVALR
                     EYLRQRPPACIRPLNQVDNLFILPVAECISLGWDSSRQTLDAQVISGEGEDNLLTLSL
                     PASASAPYAIERMAALLQQTDDPVCLVSGFVSFVDGQLTLEPQVMMTKTRAWALDAET
                     APVVVSLPSASVLPVPSTAHQLLMRCQALLIQLLHNGWRYQEQSAINQAELLANDLTA
                     VGFYRLAHVLAQFRNTESEARVEAMNNGVLLCEQLFPMLQQQG"
BASE COUNT         3544 a         3753 c         3651 g         3358 t
ORIGIN      
        1 gggattatgc gaataatgtc attattattc tgccgtctgg cattgcacct gactacttgg
       61 agcaatattc cagatttgaa ttacgcctac gtaaacattt atcactggcg gaagagtctc
      121 tctggcaaaa atgtgcacaa aaacttattg ccgcaattcc acatattcca gaatggcggc
      181 aaccattaat tgctttgtta ttacccgaaa aaccagaaat tgcacatgaa attgcccagc
      241 atctactggg gcaaaaaaaa ttaccctcgc ttgagtggtt aaaaatagtg gcgactgatg
      301 agcacattct tgcctcatta gaaaaatatc acgagccata tgccattttt gatgattact
      361 attgtggtgc gatatggtca gccaccgtat tacaggagca aggtgttgca gccctgcccc
      421 gatttgctcc ctatgccgca agtgactact gcgccgatgt gttgcgtcat atcaatcatc
      481 cgttcgcatt aacactgctt atacgtgtag ccgggcatac taaacgctgt cacgatcgaa
      541 tgacgaaagc ctgcgctgcg ttcccacacg cagcactggc agcactggcg gaacttctag
      601 tgcaaaaaga agagaatagt tggcgcatta tgctaatgac gatgtttatc tcgcaaccag
      661 cactggcaga acaggtcatt ccctggcttt cgacacccgc agttgcagtg ctgaaatcat
      721 gccagcacca actgacacag ccctcaaacc atgccagcgc cgatctactg ccagccatag
      781 tagtctcccc accatggatt tcgaaaaaga aaaaatcgcc gattccggtg ctggatttag
      841 cgccattagg catcgagcca atctgttatc tgacagaaga aatcagtaat caacttttgg
      901 cgaaatatat ctggtattca aaacacatca cggttagcca tgaagaaagt actaccaacc
      961 tgttggcaag gatgggtttt caacgacgga tcgctggtac atatattaaa gctcccgaag
     1021 cggtagttga ggcatggcta aatgaagatt attcaacctt actaagtgaa tttaaggtgt
     1081 ttcattcacc taccgggcat tattggcagt tggggatttt gacaacattg ccgctggagg
     1141 ctggagaaag cagtaaaagc atggaatgcc cttactctat ctccacatac cgataccgaa
     1201 tacgccatgt tacattttgg actcaaaggt ttacctggtt tagttaactc aattacacgc
     1261 tatccacagg aagccttgcc catcacgaat tacttcgcag cgagtgagct ggctcctgcc
     1321 gtcgcccgtg ccttcaacaa actcaaaacg ctacgcgaaa acgcccgcac ctggctgttg
     1381 aaatacccgg aacatgccct taccggcctg ctgcctgcgg cgctcggcaa agccggtgaa
     1441 gcacaggata acgcccgcgc tgccttgcgt atgcttaccg aaaacggtca tcagccatta
     1501 ctgcaagaaa tcgcccggcg ttataaccag tcggaagtaa cagacgcggt gaacgctctg
     1561 cttgcgctcg atcccttaga taatcacccg acaaaaatcc ccactcttcc gaccttttat
     1621 cagccatcga tctggacgcg cccggtatta aaagcaaacg cccaatcact gccagataac
     1681 gccctcctcc acctcggcga aatgctccgc ttccctcagg aagaggctct gtatccggga
     1741 ttattgcagg tgaaagacgc ctgtaccgct gactctctgg ctgaatttgc ctgggatctg
     1801 tttaccgcct ggcagaccgc tggcgcgccg tcgaaagaga gttgggcgtt cactgcgtta
     1861 ggcgttctcg gtaacgatga caccgcccgc aaactgacgc cattaatacg cgcctggcct
     1921 ggtgaatccc agcataaacg cgccaccgtt gggttggata ttctcgccgc tatcggtagt
     1981 gatatcgccc tgatgcagct taacggcatt gctcagaaac tgaaattcaa agcattacag
     2041 gagcgggcaa aagaaaagat tgccgacatt gccgagagcc gcgaactcac ggtagcggag
     2101 cttgaagatc ggttagcacc ggatctcggt ctggatgata acggttcgtt gctgctggat
     2161 tttggcccac gccagttcac cgtcagcttt gatgaaacct taaaaccgtt tgtgcgtgat
     2221 gtttccggca gccgcctgaa agacctgcca aaaccaaaca aaagcgatta tgagtcacag
     2281 gcgaacgatg ctgttaaccg ctacaaattg ctgaaaaaag atgcgcgtac cgtcgcggcc
     2341 cagcaggtag caaggcttga atccgccatg tgcctgcgtc gccgctggtc gccggaaaac
     2401 ttccaactct tcctggttgt gcatccgctg gttcgccact taacccgccg tctgatttgg
     2461 ggcgtttata gcgccgacaa ccagctactg gcctgtttcc gggtagcgga agataacagc
     2521 tacagcaccg ctgacgatga tcttttcacc ctgccggaag gcgatatctc tatcggcatt
     2581 cctcacgttc tggaaatatc accgacggat gctgtcgcct ttggtcagct ttttgccgac
     2641 tacgaactgc taccaccgtt ccgtcagctt gaccgtaaca gttacgccct gacagaagcc
     2701 gagcgcaacg ccagcgaact gacccgctgg gcaggcagaa aatgcccgag cggccgggtc
     2761 atggggctgg caaataaagg ctggataaag ggcgaaccac aggatggagg ctggatgatc
     2821 aaacctctcg gtcgctggtc gttaatcatg gaaatcgacg aaggttttgc ggtgggtata
     2881 tcaccagccg aacttagcgc tgagcagctc ttaagcaagc tgtggctatg ggaaggcaaa
     2941 gcggaaagct atggctgggg gagtaattca acacaggaag cgcagttctc cgtactcgat
     3001 gccatcaccg ccagcgagct aattaacgat attgaagccc tgtttgaata aggaaagagc
     3061 atggacaagg aattaccgtg gctggcggat aacgcccaac tggaactgaa atataaaaaa
     3121 ggcaaaacac cactcagtca tcgcaattgg ccgggcgaac cagtgcctgt tatcactgaa
     3181 agcatcattc agacactggg tgatgaattg ctacaaaaag ctgagaagaa aaaaaacatt
     3241 gtctggcgtt atgaaaattt ttcaccggag tggcagtccg ccattacgca ggccatcaac
     3301 ttgatcggcg aacacaaacc ctcagtcccg gcccgcacaa tggcggcact agcctttatc
     3361 gcgcaaaatg acaaccaaca gttgctcgac gaaatcgtcc aacaagaggg gctggaatat
     3421 gcgactgagg tggtgattgc acgccagttt attgcacggt gttatgagag tgatcctctg
     3481 gtagtcacat tgcagtatca gaacgaggac tatggctatg gttatcgctc agaaacctat
     3541 aacgaattcg atctccgact gcgtaaacac ctctctctgg cagaggaaag ctgctggcag
     3601 cgttgcgccg acaaactcat tgccgcactg ccaggaataa ccaaagttcg ccgccctttt
     3661 attgcgctga tcctccccga aaaaccagaa atcgccaatg agttggtaag ccttgaatgc
     3721 ccgcggactc attttcattc taaggagtgg ttaaaagttg ttgctactga ccccaaagcg
     3781 gtgagaaaac tcgaacgcta ctggagccag gatatattta gcgatcgaga agccagctac
     3841 atgtcgcatg aaaaccactt cggctacgcg gcctgcgctg cccttttgcg cgaacaagga
     3901 ctggcagtcg ttccgcgcct cgcgatatat gcccataaag aagattgcgg cagtctgctg
     3961 gtacaaatta accatccgca agtcatccgc accttgctgt tagttgctga taaaaacaaa
     4021 cccagcctgc aacgtgtggc taaatacagt aaaaacttcc cccatgcgac actcgccgca
     4081 ctggcagaac tgctggcgtt aaaagaacca ccagcccgcc caggttatcc aatcatcgaa
     4141 gacaaaaagc tgcctgcaca gcaaaaagct cgagatgaat actggcgtac gctgttacaa
     4201 acgctgatgg catcgcagcc acaactggca gcagaagtga tgccgtggtt aagtactcaa
     4261 gcccgggcag tgctgaatag ttatttatcg gcaccgccca aaccagttat tgatagcacc
     4321 gataacagcc aaatgcctga aatcctcgtt tcaccaccat ggcgtgggag aaagaaaaag
     4381 acttccatac gtcacttttc gctgcctgat cttacgcttg caccgcaaac gtacaggtcg
     4441 ccgaataaac actggacgcc ggaacagcaa actgcaattg accattttag cgccatgtct
     4501 tttgacgaac gtcttgctac gagaggagcg gatatcttct tgcgggaatt aggttttgac
     4561 gttcatagcc tggaatttaa taagcgtctt cttaatggac agttggatgc ctttgatgct
     4621 cagcaactgc aagaatacac atcaatagga ctattgcgta cttatcacgc gcatcaggcc
     4681 aaatctaaac agtaccggga agacgccacc aatgcattac tggcacaaga tagcattgcg
     4741 ttgatgggag ggtggcgaac ttttcagcac ggattttact atggtaatga acagcgttgg
     4801 gggatttgga atctgtacct tatcgcgcag atgccgcgcg aaatggcggt atcgtgctgg
     4861 caacaaattg tcgaggctga ttttcattac accggggtgg aatatttgtt aagtgtgctg
     4921 ggtaccgatg ctttgcctgg attaaacacc gcctttgccc gacatccaaa agagatattt
     4981 ccgctgctga ttcatttcgg cgcaacagaa ctggccctgc ccattgcccg cgtctggcac
     5041 ctttttgcgg gccagcgtaa tctggctcgc cagtggattt tacaatggcc ggaacatacg
     5101 gctactgcac ttatccctct tgtcttcacc aaatctagcg ataaaagcga agctgcatta
     5161 cttgccctgc gtttacttta cgaacatggg catggcgaat ggctacaaac tgtagccaat
     5221 cgttggcagc gtacagatct atggcctgcc ctggaacaat tacttaaaca gagtccaata
     5281 gagatttacc cggcacgcat tccaaaagcc cctgatttct ggcatccgca aatgtggtcc
     5341 aggccgcgcc ttatcactaa taatcaacct gttaccgatg acgctctgga aattatcggc
     5401 gaaatgctgc gctttaccca ggggggacgt ttttatagcg ggctggaaca actgaaaacg
     5461 ttctgccagc cacaaacgct ggcagctttt gcctgggatc tcttcactgc gtggcaacaa
     5521 gctggtgccc ccgcaaaaga caactgggca tttctggcgt taagtctctt tggtgacgaa
     5581 agcacggcac gggatctaac gacacatatc ctcgcctggc cacaagaagg caaatctgcc
     5641 cgtgcggtca gtggcatgaa catccttacc ctgatgaata atgatatggc gctgatgcag
     5701 ctgcatcata tatcgcaacg ggcgaaatcc cgccccttac gtgataacgc ggcggaattt
     5761 cttcaggtag tcgcagaaaa tcgcgggcta agccaggaag agttagcgga cagattagtc
     5821 ccaacactgg gccttaatga tccgcaggcg ttgagttttg attttggtcc ccggcagttt
     5881 accgttcgct tcgatgaaaa cctcaacccg gttatctttg atcagcaaaa cgttcgccag
     5941 aaaagtgttc cccggttgcg cgccgatgac gatcaactga aagcgcccga ggcactggcc
     6001 cgactaaaag ggttaaaaaa agatgctact caggtgagca aaaacctgct cccgcgtctt
     6061 gaagctgccc tacgtaccac ccgacgctgg tcgctggcag attttcattc tctgtttgtt
     6121 aatcatccct ttacccgtct ggttacccag cgattaatat ggggggttat ccggcaaatg
     6181 aaccgcgtcg tttactcaac gcctttcgtg tggccgcaga gggggagttc tgcaatgcgc
     6241 aagatgagcc aattgacctg cctgcggacg ctctgattgg cattgcccac ccgttagaaa
     6301 tgacagcaga aatgcgcagt gaatttgcac agctttttgc cgattacgaa attatgccgc
     6361 cttttcgcca gttgtcgcgc cgcacggtgc tgctcacacc tgacgagtca accagtaaca
     6421 gcctgactcg ctgggaaggt aaatccgcta ccgttgggca acttatggga atgcgataca
     6481 aaggctggga gtcaggttat gaggacgcat ttgtctatga cctgggtgag taccggctag
     6541 tccttaagtt ttcacccggt tttaaccact acaatgttga tagcaaagcg ctaatgagct
     6601 tccgttctct tcgagtgtac cgtgacaata aatccgtcac ttttgccgaa cttgatgtgt
     6661 ttgatttgag tgaggcgtta agcgcacctg acgtcatttt ccattaaaac aaagcgggca
     6721 gtgatgcgac tgcccgttat ctacacgact taccagcggg gaaagcgatg atcgtgcaaa
     6781 aagagctggt tgctatttac gattatgagg tccctgtacc tgaagatccg ttttccttca
     6841 gacttgagat ccataaatgc tctgaattat ttacaggttc cgtctaccga ctggaacggt
     6901 tccggctacg tccaacattt catcaacgtg atcgagagga tgttgacccg ctaataaatg
     6961 atgcgttgat ttatataaga gatgagttta ttgatgagca aaaattacga ggcgaatcac
     7021 ctgaacctgt aatagtaatt tttaattgta aactacagaa tatattcaac caacaaatag
     7081 aattatatac tctagataat tcaagtcgca gcacatgcaa cttgaagtat gacgagtata
     7141 gctttattaa aggggggaac ggtattcccc cttagatgcg tttcaatttg cccgttactt
     7201 caacaaatca atcaaccgta acagagccac taatagctcc agtacagcaa tgagcacggt
     7261 taacttttgt gtcagcgtgt ccatccgctc tccttattaa ggagcacaac accagcacct
     7321 tacccttaca ctgcctgtcg atactggttt acgtttgttg agtcgccatg ttgcactccg
     7381 gatcagagca ctacctgcgg caccacccgt ccggtagcca ggacttgaaa taggttcgat
     7441 ccggcgcaac ttcccaacag tcaggattat aaatctcatt atcttaattg caacactgtc
     7501 cagaggtggt tataattcgt tcgttggaac acgatgttgc acaggcagtg gtgttaggcc
     7561 tgaaaatagt aaataaaaga acctgcctca ccagcagttt tttttattta ctgtgatctg
     7621 ctttccagat atttttcgct caaacaacta atgcgccaaa catttattgc gcgtaaaata
     7681 tcgtttattt cattaataca tttcagggat gaatatatgt caccgcagaa taatcatctg
     7741 cagcgtccgc ctgctgctgt gttatacgcc gatgaactgg caaaattaaa acaaaatgat
     7801 aacgcacctt gcccgcccgg ttggcagtta agtttgcctg cggcccgtgc ttttatcctt
     7861 ggcgatagcg cgcaaaatat tagccgtaaa gtggtgatta gtccctccgc tgtcgaacgt
     7921 atgttagtca ctcttgccac cggacgcggt ttgatgttgg tgggggaacc tggcaccgca
     7981 aaatctctcc tgtctgaatt actggcaacc tctattagcg gcgatgccgg gttaaccatc
     8041 cagggcggcg catctactac cgaagatcaa atcaagtacg gctggaacta cgccctgctt
     8101 atcaatcatg gtccatcaac ggaagcgctg gtccctgccc cgctttacca gggaatgcgc
     8161 gacggcaaaa tcgtccgttt tgaagagata acgcgtacac cgctggaagt gcaagactgt
     8221 ctgttaggta tgctttctga cagggtgatg acggtgccgg aactcactgg tgaagccagt
     8281 cagctctatg cgcgagaagg ctttaatatt attgccactg ccaatacccg cgatcgcggc
     8341 gtcaatgaaa tgagtgctgc gctaaagcgt cgcttcgatt ttgaaactgt ctttccgatt
     8401 atggatttcg cccaggagct ggagcttgtc gccagtgctt cggcgcgttt gctggcccat
     8461 agcggtattc cgcataaagt gcctgacgcg gtactggagt tactggtccg caccttccgc
     8521 gatctgcgtg ccaacggcga aaagaaaacg tcaatggata cactaacagc gattatgtcc
     8581 accgctgaag ccgtgaacgt ggcccacgcc gtaggcgtcc gcgcctggtt tttagcgaat
     8641 cgcgcaggtg agccagccga cctggtggac tgcattgccg gaaccatcgt caaagataac
     8701 gaagaagatc gcgctcgtct gcgccgttac ttcgaacagc gtgtcgccac acataaagaa
     8761 gctcactggc aggcttatta tcaagcccgc caccgcctgc cgtgaggaaa gatgcatgag
     8821 cgagccgtta attgtcggca tccggcatca tagtccggcc tgcgcccggc tggtgaaatc
     8881 gttaatcgaa agccagcggc cacgatacgt gttgattgaa ggccctgctg attttaatga
     8941 ccgggtagac gaactgtttt tagcccacca gcttccggta gctatttaca gttattgcca
     9001 gtatcaggac ggtgcagccc ccgggcgtgg tgcctggacg ccatttgctg aattttcgcc
     9061 ggagtggcag gcgctacaag ccgatcgtcg tattcaggca caaacttact tcatcgattt
     9121 gccttgctgg gcgcagagtg aagaagagga cgattcgcct gatacgcaag aggaaagcca
     9181 gaccttgctg ctgcgtgcca cccgcatgga taacagcgac accctgtggg atcacttgtt
     9241 cgaagatgaa agccagcaaa ctgcattacc ctctgcactg gcgcactatt ttgcccaact
     9301 gcggggcgac tcccccggcg atgctctaaa tcgtcagcgc gaagccttta tggcccgctg
     9361 gattggatgg gcgatgcagc aaaataatgg cgacgtgtta gttgtctgcg gtggctggca
     9421 cgctccggca ctggcaaaaa tgtggcgcga atgtccgcag gtaattaaca cgccagaatt
     9481 gccgtcgctg gcagatgccg ttacaggttg ttatctcacc ccctacagtg aaaagcgcct
     9541 tgatgtgctg gcaggatacc tttcaggaat gcctgccccg gtctggcaaa actggtgctg
     9601 gcagtggggc ttacagcagg cgggagaaca actgctaaaa acggttctca cccgtttgcg
     9661 ccagcacaag ttgcctgctt cgaccgcgga tatggctgcc gctcatctgc atgcgatggc
     9721 actggcacag ttgcgcggtc atacactacc gttacgcact gactggctgg atgccatagc
     9781 aggatcgctg attaaagaag ccctgaatgc gccgttgccg tggagctatc gcggcgttat
     9841 tcatcccgat accgatccga ttctgctaac gttgatagac acattagcgg gtgacggatt
     9901 cggtaaactt gccccttcta cgccacaacc gcctctgcca aaagatgtca cttgcgaact
     9961 ggaacgtacc gcaatctctc ttccggcgga gcttacctta aatcgcttta accccaatgg
    10021 actagcgcaa agtcaggtgt tacatcggct ggcaatactg gagatcccag ggattgtacg
    10081 ccagcaggga agcacactga cacttgcagg caacggtgaa gaacgctgga aattaacccg
    10141 cccgcttagc cagcatgcgg cattgattga ggccgcctgc tttggtgcca cactcctgga
    10201 agccgcacgc cataaattag aagccgatat gctggacgcg ggtggaatcg gcagcattac
    10261 cacatgtctt agccaagcgg cgtcagcggg tctggcgtcc ttcagtcaac aattactgga
    10321 gcaactcaca ttattaatcg cccaggaaaa tcaatttgcc gaaatgggcc aggcgctgga
    10381 agtgctatat gccttatggc ggctggatga aattagcggt atgcaaggcg cgcagatatt
    10441 acaaacgacg ttatgcgcgg ctatcgatcg cacgctgtgg ctgtgtgaat ccaacggcag
    10501 accggatgaa aaggagtttc acgctcactt gcatagctgg caagcgttgt gtcatatcct
    10561 gcgcgatcta catagcggtg tgcaactccc cggcgtttcg ctttctgcgg cggtagcctt
    10621 gctggagcga cacagtcagg cgattcatgt cctggcactg gatcgcggcg cgactcttgg
    10681 tgcgctgatg cgtctggaac atcccaacgc tagtgccgaa gcggcgctga cgatgctggc
    10741 gcagttatcc ccggcacaat ccggcgaggc gctgcacggt ttgctggcat tagcccgtca
    10801 tcaactggcc tgtcagccgg catttatcgc cggtttcagc agtcatttaa atcaactaag
    10861 tgatgccgat tttaccaatg ccctgcccga tttacgcgcg gcaatggcct ggctaccgcc
    10921 acgagaacgc gggacgctgg cgcatcaggt gcttgagcat tatcaactgg cgcaacttcc
    10981 cgtttcggca ctgcaaatgc cgttgcattg tccaccgcaa gccattgcac atcatcaaca
    11041 actcgaacag caggcactgg catcgctgca acactgggga gttttccatg tctgaactga
    11101 acgatcttct gaccacccgt gagctacaac gctggcgatt aattcttggc gaagcggcag
    11161 aaacgacgct ttgtgggctg gatgacaacg cccggcagat agatcacgcg ctggagtggc
    11221 tgtatgggcg cgatcctgaa cggctccagc gtggtgaacg ctctagtgga ttaggtggct
    11281 caaatctcac cactcctgag tggatcaaca gtattcacac gctgtttccg caacaggtga
    11341 ttgagcggct ggaaagcgat gccgtactgc gctacggcat tgaagatgtg gtgacaaatc
    11401 tcgacgtgct ggaacgtatg cagccttccg aaagcctgct acgcgccgtt ttgcacacca
    11461 aacatctgat gaaccccgaa gtactggctg ccgcccgcag gattgtgcgc caggttgtgg
    11521 aagaaattat ggcgcgactg gcaaaggaag ttcgtcaggc tttttctggt gtccgcgatc
    11581 gtcgtcgccg ctcatttatt ccactggcgc gaaactttga tttcaaaagt actctgcgcg
    11641 ccaacctgca acactggcac ccgcaacacg gcaagttgta tatcgaattc ccccgcttta
    11701 acagccgcat taaacgccaa agcgagcaat ggcaactggt cttactggtt gatcaaagcg
    11761 gatcgatggt cgattcggtg atccactctg cggtgatggc ggcctgttta tggcagttac
    11821 ccggcattcg tacccatctg gtggcgtttg acacaagcgt cgttgatctc acggcagacg
    11881 ttgccgatcc ggtagagtta ttaatgaaag tgcaattagg cggcggaacc aatatcgcca
    11941 gtgccatgga gtatggtcgg caacttattg aacaacctgc aaaaagcgtc attatcctcg
    12001 tgagcgattt ttatgaaggg ggttcatcat cattgctgac gcaccaagtg aaaaagtgtg
    12061 tccagagcgg catcaaagtg ctgggactgg cagcgctcga tagcacagca acaccttgct
    12121 atgaccgcga tacggcccag gcgctggtta atgtcggcgc acaaatagcc gccatgacac
    12181 cgggcgagct ggcatcatgg cttgcggaga atcttcagtc atgaattcac tacgtccgga
    12241 attattagaa ctgacaccgc aggcactgac ggcgttaagc aatgccggtt ttgttaagcg
    12301 cagccttaag gaactggaaa atggcaacgt cccggaaatc agccatgaga acagcgcttt
    12361 aatcgccacc ttcagtgacg gtgtccgtac ccagctggcg aacggccagg cactgaaaga
    12421 ggctcagtgc acctatgggg ccagcggcat gtgccgtcat cgcgtgatgc tggtgttaag
    12481 ttatcaacga ctttgtgcta ccgcccagcc tacgggaaaa gaagaggaat gggatccggc
    12541 aatctggctg gaagaactgg ctacccttcc cgatgccacc cgcaaacgcg cacaggcgct
    12601 ggtcgctaaa agcatcacca ttgagttgtt ctgtgcgccg ggtgaaattc cctctgcccg
    12661 cttaccgatg agcgatgtgc gtttttattc ccgcagcagt attcgtttcg cccgttgtga
    12721 ttgtattgaa ggcacacttt gcgaacatgt cgtactggca gtacaggcct ttgtccaggc
    12781 aaaagcgcag caagcggaat tgactcattt aatctggcag atgcgcagcg aacacgtcac
    12841 atcatctgac gatccgtttg ccagcgaaga aggcaaaacg tgtcgtcaat atgttcaaca
    12901 actaagccag gcattatggc tgagcggcat cagccagccg cttattcact acgaggccgc
    12961 tttcagccgc gcgcagcaag cagcggaacg ctgcagctgg cgatgggtga gtgaatcgct
    13021 acggcaactg cgcgccagcg ttgatgcctt ccacgcccgc gccagccact atcatgccgg
    13081 agaatgctta cgtcagcttg cggcattaaa cagtcgatta aattgcgcac aagagatggc
    13141 ccggagcgac agtgttggtg aagttcctcc tgtgccgtgg cgcacggtcg ttggctctgg
    13201 cattgccgga gaagcaaagc ttgatcatct gcggctggtg tctttaggta tgcgttgctg
    13261 gcaggatatt gagcattatg gtttacgcat ctggtttacc gatcccgaca ccggcagtat
    13321 tttgcacctt tcgcgcagtt ggccgcgaag tgaacaggaa gactcaccgg cagctacgcg
    13381 tcggctgttt agttttcagg ctggcgcact ggcgggtggg caaattgttt cacaagcagc
    13441 aaaacgcagt gccgatggcg agctgctgtt agctacccgc aaccgcttaa gcagcgttgt
    13501 accgctgtcg cctgatgcct ggcaaatgtt gagcgcgccg ttacgccagc cgggcattgt
    13561 ggctttgcgg gaatatttac gccagcgtcc ccccgcctgc atacggcctc ttaatcaggt
    13621 cgataactta tttattctgc cggtcgctga gtgtatttcg ctcggctggg atagcagccg
    13681 ccagacgctg gatgcgcagg taatcagcgg cgaaggtgaa gataatctgc tgacgttatc
    13741 actgccagcc tcagccagcg cgccttatgc cattgaacgc atggcggcgc ttttgcaaca
    13801 aacagacgac cccgtgtgtc tggtttctgg ctttgtcagt tttgttgatg ggcaattgac
    13861 actggaacca caggtaatga tgacaaagac tcgtgcctgg gcgctggacg cagaaactgc
    13921 gcctgtggtg gtatcactac cttctgccag cgttctgcct gtgccgtcta ctgctcatca
    13981 gttgctgatg cgctgccagg cgttacttat tcaactgctc cataacggct ggcgctatca
    14041 ggaacagagc gctattaatc aggcagagtt gctggcgaat gacctcaccg cggtgggttt
    14101 ttatcggctg gcacatgtgt tggcacaatt tcgtaataca gaaagcgagg cacgggtaga
    14161 agcaatgaat aacggtgttt tgctttgcga acaattattc cccatgcttc agcaacaagg
    14221 atgaaatagt gctttttact aagagttcta ctccagttcc ggactgctca cgccacggta
    14281 ttaggcatat cctatatagc ccctgg
//
LOCUS       DAJTRC010000091        14299 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.091, whole genome shotgun sequence.
ACCESSION   DAJTRC010000091 DAJTRC010000000
VERSION     DAJTRC010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14299)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14299)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14299
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.091"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..132)
                     /locus_tag="OO357_003090"
     CDS             complement(<1..132)
                     /locus_tag="OO357_003090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5887948.1"
                     /translation="MFLDYFALGVLIFVFLVIFYGIIILHDIPYLIAKKRNHPHADAI
                     "
     gene            315..458
                     /locus_tag="OO357_003091"
     CDS             315..458
                     /locus_tag="OO357_003091"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887949.1"
                     /translation="MNNSSKTSTVQIKRIKPSIIYRLLLIGLGSPMVIYGLVRPLTIE
                     TRD"
     gene            complement(677..2215)
                     /gene="aldB"
                     /locus_tag="OO357_003092"
     CDS             complement(677..2215)
                     /gene="aldB"
                     /locus_tag="OO357_003092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001551836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase AldB"
                     /protein_id="HCR5887950.1"
                     /translation="MTNNPPSAQIKPGEYGFPIKLKARYDNFIGGEWVAPADGEYYQN
                     LTPVTGQLLCEVASSGKRDIDLALDAAHKVKDKWAHTSVQDRAAILFKIADRMEQNLE
                     LLATAETWDNGKPIRETSAADVPLAIDHFRYFASCIRAQEGGISEVDSETVAYHFHEP
                     LGVVGQIIPWNFPLLMASWKMAPALAAGNCVVLKPARLTPLSVLLLMEIVGDLLPPGV
                     VNVVNGAGGEIGEYLATSKRIAKVAFTGSTEVGQQIMQYATQNIIPVTLELGGKSPNI
                     FFADVMDEEDAFFDKALEGFALFAFNQGEVCTCPSRALVQESIYERFMERAIRRVESI
                     RSGNPLDSVTQMGAQVSHGQLETILNYIDIGKKEGADVLTGGRRKLLEGELKDGYYLE
                     PTILFGQNNMRVFQEEIFGPVLAVTTFKTMEEALELANDTQYGLGAGVWSRNGNLAYK
                     MGRGIQAGRVWTNCYHAYPAHAAFGGYKQSGIGRETHKMMLEHYQQTKCLLVSYSDKP
                     LGLF"
     gene            complement(2380..3531)
                     /gene="yiaY"
                     /locus_tag="OO357_003093"
     CDS             complement(2380..3531)
                     /gene="yiaY"
                     /locus_tag="OO357_003093"
                     /EC_number="1.1.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine dehydrogenase"
                     /protein_id="HCR5887951.1"
                     /translation="MASSTFFIPSVNVIGADSLTDAMNMMADYGFTRTLIVTDNMLTK
                     LGMAGDVQKALEERNIFSVIYDGTQPNPTTENVAAGLKLLKENNCDSVISLGGGSPHD
                     CAKGIALVAANGGDIRDYEGVDRSAKPQLPMIAINTTAGTASEMTRFCIITDEARHNK
                     MAIVDKHVTPLLSVNDSSLMIGMPKSLTAATGMDALTHAIEAYVSIAATPITDACALK
                     AVTMIAENLPLAVEDGSNAKAREAMAYAQFLAGMAFNNASLGYVHAMAHQLGGFYNLP
                     HGVCNAVLLPHVQVFNSKVAAARLRDCAAAMGVNVTGKNDAEGAEACINAIRELAKKV
                     DIPAGLRDLNVKEEDFAVLATNALKDACGFTNPIQATHEEIVVIYRAAM"
     gene            complement(3721..5565)
                     /gene="selB"
                     /locus_tag="OO357_003094"
     CDS             complement(3721..5565)
                     /gene="selB"
                     /locus_tag="OO357_003094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HCR5887952.1"
                     /translation="MIIATAGHVDHGKTTLLQAITGVNADRLPEEKKRGMTIDLGYAY
                     WPQPDGRVPGFIDVPGHEKFLSNMLAGVGGIDHALLVVACDDGVMAQTREHLAILQLT
                     GNPMLTVALTKADRVDEARVDEVERQVKEVLREYGFAEAKLFITAATEGRGIDALREH
                     LLQLPEREHASQHSFRLAIDRAFTVKGAGLVVTGTALSGEVKVGDSLWLTGVNKPMRV
                     RALHAQNQPTETANAGQRIALDIAGDAEKEQINRGDWLLADVPPEPFTRVIVELQTHA
                     PLTQWQPLHIHHAASHVTGRVSLLEDNLAELVFDTPLWLADNDRLVLRDISARNTLAG
                     ARVVMLNPPRRGKRKPEYLQWLASLARAQNDSEALAVHLQRGAVNLPDFAWARQLNGE
                     GMRELLQQPGYIQAGYSLLNAPVAARWQRKILDTLATYHEQHRDEPGPGRERLRRMAL
                     PMEDEALVLLLIEKMRESGDILSHHGWLHLPDHKAGFSEEQQAIWQKAEPLFGDEPWW
                     VRDLAKETGTDEQAMRLTLRQAAQQGIITAIVKDRYYRNDRIVEFANMIRDLDQECGS
                     TCAADFRDRLGVGRKLAIQILEYFDRIGFTRRRGNDHLLRDALLFPEK"
     gene            complement(5562..6953)
                     /gene="selA"
                     /locus_tag="OO357_003095"
     CDS             complement(5562..6953)
                     /gene="selA"
                     /locus_tag="OO357_003095"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HCR5887953.1"
                     /translation="MTTETRSLYSQLPAIDRLLRDSSFLSLRDTYGHTRVVELLRQML
                     DEAREVIRGSQTLPAWCENWAQEVDARLTKEAQSALRPVINLTGTVLHTNLGRALQAE
                     AAVEAVAQAMRSPVTLEYDLDDAGRGHRDRALAQLLCRITGAEDACIVNNNAAAVLLM
                     LAATASGKEVVVSRGELVEIGGAFRIPDVMRQAGCTLHEVGTTNRTHANDYRQAVNEN
                     TALLMKVHTSNYSIQGFTKAIDEAELVALGKELDVPVVTDLGSGSLVDLSQYGLPKEP
                     MPQELIAAGVSLVSFSGDKLLGGPQAGIIVGKKEMIARLQSHPLKRALRADKMTLAAL
                     EATLRLYLHPEALSEKLPTLRLLTRSAEVIQIQAQRLQAPLVAHYGAEFAVQVMPCLS
                     QIGSGSLPVDRLPSAALTFTPHDGRGSHLESLAARWRELPVPVIGRIYDGRLWLDLRC
                     LEDEQRFLEMLLK"
     gene            complement(7051..7659)
                     /locus_tag="OO357_003096"
     CDS             complement(7051..7659)
                     /locus_tag="OO357_003096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HCR5887954.1"
                     /translation="MKLVGSYTSPFVRKLFILLLEKGITFEFINELPYNADNGVAQFN
                     PLGKVPVLVTEEGECWFDSPIIAEYIELMNVAPAMLPRDPLESLRVRKIEALADGIMD
                     AGLVSVREQARPAAQQSEDELLRQREKINRSLDVLEGYLVDGTLKTDTVNLATIAIAC
                     AVGYLNFRRVAPGWCVDRPHLVKLVENLFSRESFARTEPPKA"
     gene            complement(7730..8815)
                     /locus_tag="OO357_003097"
     CDS             complement(7730..8815)
                     /locus_tag="OO357_003097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5887955.1"
                     /translation="MDLLIVLTYVALATAALGGVFLVSGLILLMNYNHPYTFTAQKAV
                     IAIPITPQVTGIVTEVTDKNNQLIQKGEVLFKLDPVRYQARVDRLQADLMTATHNIKT
                     LRAQLTEAQANTTQVSAERDRLFKNYQRYLKGSQAAVNPFSERDIDDARQNFLAQDAL
                     VKGSVAEQAQIQSQLDSMVNGEQSQIVSLRAQLTEAKYNLEQTVIRAPSNGYVTQVLI
                     RPGTYAAALPLRPVMVFIPEQKRQIVAQFRQNSLLRLKPGDDAEVVFNALPGQVFHGK
                     LTSILPVVPGGSYQAQGVLQSLTVVPGTDGVLGTIELDPNDDIDALPDGIYAQVAVYS
                     DHFSHVSVMRKVLLRMTSWMHYLYLDH"
     gene            complement(8818..9180)
                     /locus_tag="OO357_003098"
     CDS             complement(8818..9180)
                     /locus_tag="OO357_003098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3302 domain-containing protein"
                     /protein_id="HCR5887956.1"
                     /translation="MFLNYFALGVLIFVFLVIFYGIIAIHDIPYLIAKKRNHPHADAI
                     HTAGWVSLFTLHVIWPFLWIWATLYQPERGWGMQSHVASQEKATEPEIAALSDRISRL
                     EHQLAAEKKTDYSTFPEI"
     gene            9717..11630
                     /gene="mtlA"
                     /locus_tag="OO357_003099"
     CDS             9717..11630
                     /gene="mtlA"
                     /locus_tag="OO357_003099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HCR5887957.1"
                     /translation="MSSDIKIKVQSFGRFLSNMVMPNIGAFIAWGIITALFIPTGWLP
                     NETLAKLVGPMITYLLPLLIGYTGGKLVGGKRGGVVGAITTMGVIVGADMPMFLGSMI
                     AGPLGGWCIKHFDRWVDGKIKSGFEMLVNNFSAGIIGMILAILAFLGIGPIVEALSKM
                     LAAGVNFMVVHDMLPLASIFVEPAKILFLNNAINHGIFSPLGIQQSHELGKSIFFLIE
                     ANPGPGMGVLLAYMFFGRGSAKQSAGGAAIIHFLGGIHEIYFPYVLMNPRLILAVILG
                     GMTGVFTLTILGGGLVSPASPGSILAVLAMTPKGAYFANIAGVCAAMAVSFVVSAILL
                     KTSKVKEEDDIEAATRRMQDMKAESKGTSPLSAGDVTNDLSHVRKIIVACDAGMGSSA
                     MGAGVLRKKIQDAGLSQISVTNSAINNLPPDVDLVITHRDLTERAMRQVPQAQHISLT
                     NFLDSGLYTSLTERLVAAQRHTENEVKVKDSLKDSFDDSSANLFKLGAENIFLGRKAA
                     TKEEAIRFAGEQLVKGGYVEPEYVQAMLDREKLTPTYLGESIAVPHGTVEAKDRVLKT
                     GVVFCQYPEGVRFGEEEDDIARLVIGIAARNNEHIQVITSLTNALDDESVIERLAHTT
                     SVDEVLELLAGRK"
     gene            11725..12873
                     /gene="mtlD"
                     /locus_tag="OO357_003100"
     CDS             11725..12873
                     /gene="mtlD"
                     /locus_tag="OO357_003100"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709374.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HCR5887958.1"
                     /translation="MKALHFGAGNIGRGFIGKLLADAGIQLTFADVNQVVLDALNARH
                     SYQVHVVGETEQVDTVSGVNAVSSIGDDVVDLIAQVDLVTTAVGPVVLERIAPAIAKG
                     LVKRKEQGNESPLNIIACENMVRGTTQLKGHVMNALPEDAKAWVEEHVGFVDSAVDRI
                     VPPSASATNDPLEVTVETFSEWIVDKTQFKGALPNIPGMELTDNLMAFVERKLFTLNT
                     GHAITAYLGKLAGHQTIRDAILDEKIRAVVKGAMEESGAVLIKRYGFDADKHAAYIQK
                     ILGRFENPYLKDDVERVGRQPLRKLSAGDRLIKPLLGTLEYSLPHKNLIQGIAGAMHF
                     RSEDDPQAQELAALIADKGPQAALAQISGLDANSEVVSEAVTAYKAMQ"
     gene            12873..13460
                     /gene="mtlR"
                     /locus_tag="OO357_003101"
     CDS             12873..13460
                     /gene="mtlR"
                     /locus_tag="OO357_003101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol operon repressor MtlR"
                     /protein_id="HCR5887959.1"
                     /translation="MVDQAQDTLRPNNRLSDMQATMEQTQAVENRVLERLNAGKTVRS
                     FLITAVELLTEAVNLLVLQVFRKDDYAVKYAVEPLLDGDGPLGDLSVRLKLIYGLGVI
                     NRQEYEDAELLMALREELNHDGNEYAFTDDEILGPFGELHCVAALPPPPQFEPADSSL
                     YAMQIQRYQQAVRSTMVLSLTELISKISLKKAFQK"
     gene            complement(13472..13681)
                     /locus_tag="OO357_003102"
     CDS             complement(13472..13681)
                     /locus_tag="OO357_003102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887960.1"
                     /translation="MGKLGENVPLLIDKAVDFMASSQAFREYLKKLPPRNAIPSGIPD
                     ESVPLYLQRLEYYRRLYRPKQVEGQ"
     gene            13965..>14299
                     /locus_tag="OO357_003103"
     CDS             13965..>14299
                     /locus_tag="OO357_003103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibL family ribosome-associated protein"
                     /protein_id="HCR5887961.1"
                     /translation="MKEVEKNEIKRLSDRLDAIRHQQADLSLVEAADKYAELEKEKAT
                     LEAEIARLREVHSQKLSKEAQKLMKMPFQRAITKKEQADMGKLKKSVRGLVVVHPMTA
                     LGREMGLEE"
BASE COUNT         3500 a         3805 c         3598 g         3396 t
ORIGIN      
        1 aatggcgtcg gcatgaggat gattacgttt tttagcaatc aggtagggaa tatcatgtaa
       61 aataataatc ccatagaata tcaccagaaa tacaaaaata agcactccca gtgcaaaata
      121 gtccaggaac atatttccct caaagaatat aaaaaagaac aattaacgca tattatgaaa
      181 tgccatgatg cagtgcaatt tccttattta tataacgaca tattgttttt tatatacttt
      241 ctgaaatgtg ctgtctggtt tttcaacagg acgaggttta tcatattgaa atagtggaac
      301 tattggccaa actaatgaat aactccagta aaacatccac agtacagatt aagcgtatta
      361 aaccttcaat tatctaccgt ttattgctga ttggcctcgg atcaccaatg gtgatttacg
      421 gcctggttcg cccgctcacc atcgaaacgc gagattaaac tcctgacaaa acacgcccca
      481 gaaacgcact tctgttggcg tagtctgggt tattgcgcac gtaggtttaa aacgtaaatt
      541 gtaacaacgt gcgctttgtt tatgccggat gcggcgtgta cgccttatcc ggcctacggt
      601 caggttcccg taggcatgat aagacgcgta agcgtcgcat caggcaatga atacccaatg
      661 cgaccagctt cttatatcag aacagcccca acggtttatc cgagtagctc accagcaggc
      721 acttggtttg ctggtaatgc tccagcatca tcttgtgggt ttcgcgaccg atacctgatt
      781 gtttgtagcc accaaacgca gcatgtgccg ggtaagcgtg ataacagttg gtccacacgc
      841 gcccagcctg tatgccgcgc cccatcttat aggccagatt accgttgcgg ctccagacgc
      901 ccgcgcccag gccatattgc gtatcgttcg ccagctccag cgcttcttcc atcgttttga
      961 aggtggtcac cgccagcacc gggccaaaaa tctcctcctg gaacacccgc atattgttct
     1021 gaccaaacag aatcgtcggt tcgaggtagt agccgtcttt cagttcacct tccagcagct
     1081 tgcgccgccc gcctgtgagc acgtcagcgc cctctttttt accgatatca atgtagttga
     1141 ggatggtttc cagttgcccg tgagaaacct gcgcgcccat ttgcgtcacg ctgtcgagcg
     1201 ggttaccgct gcgaatgctt tcgacacggc ggatggcgcg ttccataaag cgttcgtaga
     1261 ttgactcctg cactaaggca cgactcggac aggtgcaaac ttcgccctgg ttaaaggcaa
     1321 acagtgcaaa gccttccagc gctttatcga aaaaggcatc ttcttcatcc atcacatcag
     1381 caaagaagat atttggcgac ttaccgccca actccagcgt caccggaata atgttttgcg
     1441 ttgcgtattg cataatttgt tggcccactt ccgttgagcc ggtaaacgcc actttggcga
     1501 tgcgtttcga ggtcgccaga tattcgccaa tttccccacc tgcgccattg accacgttca
     1561 ccacgcccgg cggcagtaaa tcaccgacaa tttccattag cagcagtaca gaaagcgggg
     1621 taagacgtgc gggtttcagc accacacagt tgcccgccgc cagcgcggga gccattttcc
     1681 agctcgccat cagcagcggg aagttccacg ggataatctg ccccaccacg cctaacggtt
     1741 catggaaatg ataggccacg gtttcgctat caacttcact gatcccacct tcctgcgccc
     1801 gaatacacga ggcgaaatag cggaaatggt caatcgccag cggtacatcc gcagcactgg
     1861 tttcgcgaat gggtttgccg ttatcccagg tttcagctgt cgctaacagc tcgaggtttt
     1921 gttccattcg atcggcaatc ttaaacagaa tcgccgcgcg atcctgcacc gaggtgtgcg
     1981 cccatttatc tttcactttg tgcgcagcat ccagcgccag atcgatgtct cgtttgcccg
     2041 aagacgccac ttcgcacagc agctgcccgg tcaccggcgt cagattctgg taatactcac
     2101 catcggcagg ggctacccat tcaccgccaa taaagttgtc gtagcgggct tttaacttga
     2161 tggggaaacc atactcgccg ggcttaatct gtgctgaagg gggattattg gtcatgatcg
     2221 tctccttgcg gtgtgaggta taacaagggt agacgtgact ggcgaaatct tcgccagtcg
     2281 gtaacagctt tacgacagct atcacgaatt tacgggcaag gattacagag atagttgaaa
     2341 aagcggctga caatttgcca gccgttgttg gaatgatgat tacattgcgg cgcgataaat
     2401 caccacaatt tcttcgtgag ttgcctggat cgggttagta aagccacagg catctttcag
     2461 ggcattagtc gccagaaccg cgaaatcttc ttctttcacg ttcaggtcgc gtaggcctgc
     2521 cgggatatcc actttcttcg ccagttcacg gatggcgtta atgcaggctt cagcaccttc
     2581 cgcgtcgttt ttacctgtca cgttcacgcc cattgcagcg gcacagtcac gcagacgtgc
     2641 agcggcgact ttgctgttga atacctgaac atgcggcagc agaacggcgt tacatacacc
     2701 gtgtggcagg ttgtagaaac cgcccagctg gtgcgccatc gcatgaacat aacccagaga
     2761 agcattattg aacgccatac cggcaaggaa ctgggcataa gccattgctt cacgcgcttt
     2821 cgcattactg ccatcttcaa cggctaacgg caggttttcg gcaatcatgg tcacggcttt
     2881 cagtgcacaa gcgtcagtga tcggcgtggc ggcaatagaa acatacgctt cgatagcgtg
     2941 cgttaaggca tccatacccg ttgcggcggt cagtgacttc ggcataccaa tcatcagaga
     3001 ggagtcattg acagaaagca gcggagtcac gtgcttatca acaatcgcca ttttgttatg
     3061 acgcgcttcg tcagtgatga tgcagaaacg ggtcatttca gaggccgtac ccgccgtggt
     3121 attgatggcg atcatcggca gctgcggttt tgcagagcgg tcaacgcctt cgtaatcgcg
     3181 aatatcgccg ccattggctg ccaccagcgc aatacctttt gcgcagtcgt gtggagaacc
     3241 accgcctaag gagatcacgc tatcgcaatt attttcttta agtaatttca aacctgcggc
     3301 gacgttttcc gtggtggggt taggttgggt gccatcataa ataacgctaa aaatattgcg
     3361 ttcttccagt gctttttgca catcacccgc catacctaat ttcgttaaca tattgtcagt
     3421 gacaattaag gtacgggtaa atccataatc tgccatcata ttcattgcat cagtcaatga
     3481 atcagcgccg atgacattca cagaaggaat aaagaaagtt gaagatgcca taacacactc
     3541 ctgaaagtgt aaaaaagggt gggcagctta ctgaggattt tcattcgaaa acatgatcat
     3601 tattattaat tatcaaaatt aatctaataa aaagcacatt gtattcatta aacaatgtgc
     3661 tttatttaaa atcataagaa atttgtatta atttatttta aaaattaatt aatcatttcc
     3721 ttatttttcc ggaaataata atgcgtcgcg caataaatga tcatttccac gacgacgcgt
     3781 aaagccaatg cggtcaaaat attccagaat ctgaattgcc agctttcggc ctacgcctaa
     3841 gcgatcgcgg aaatccgccg cgcaggttga accacactcc tgatcgagat cgcggatcat
     3901 attggcaaat tcgacaatcc gatcgttacg gtaataacga tctttgacga tcgcggtaat
     3961 tattccttgc tgcgccgcct ggcgtagcgt caggcgcatt gcctgctcgt cggtacccgt
     4021 ctcttttgcc agatcacgca cccaccacgg ctcgtcacca aacagcggct ctgctttttg
     4081 ccagatggcc tgctgctctt cgctgaagcc cgctttgtga tccggcagat gcagccaacc
     4141 gtgatgactg aggatgtcgc cgctttcgcg catcttttca atcagcaaca gtactagcgc
     4201 ttcatcttcc attggcaacg ccatacgtcg cagacgttcg cgcccaggcc caggttcatc
     4261 gcgatgttgc tcatgataag tcgctaatgt gtcgagaatt ttccgttgcc agcgagcggc
     4321 aaccggcgca ttcaacaagc tataaccagc ctgaatatag ccaggctgtt gcagcagttc
     4381 gcgcatccct tcgccgttga gctggcgcgc ccaagcgaaa tcaggaagat taaccgcgcc
     4441 gcgttgcagg tgaacggcta acgcttcgct atcattctgt gccctggcga gggaagccag
     4501 ccattgcaga tactctggtt tacgtttacc gcgacgcggc gggttaagca tcacgacgcg
     4561 cgctccggcc agcgtgttgc gggcagagat atcgcgcaat accaggcggt cgttatctgc
     4621 cagccataac ggggtgtcga agaccagttc agcaaggtta tcttccagca gtgaaacgcg
     4681 tcccgtgacg tggctggcgg cgtggtgaat atgcagcggc tgccactggg tcagcggtgc
     4741 atgggtttga agctcgacaa tcacccgtgt gaacggctct ggcggcacat cggcaagcag
     4801 ccagtcgcca cggttaatct gctctttttc cgcatcaccc gcgatgtcaa gcgcgatacg
     4861 ctgcccggca ttggcggttt ctgttggctg gttttgcgca tgcagcgcac gtacacgcat
     4921 cggtttattt acaccagtca gccagagtga atcgcctacc ttcacttccc cgcttaacgc
     4981 cgtaccggtg acgaccagcc cggcaccttt tacggtaaat gcgcggtcaa tcgcgaggcg
     5041 gaaactatgt tggctggcgt gctcgcgttc cggcaactga agcagatgct cgcgcagggc
     5101 atcaattccc cgaccttcgg ttgctgcggt gataaacagt tttgcctcag caaaaccgta
     5161 ttcccgcaga acctccttta cctggcgttc aacctcatca acacgcgctt cgtccacgcg
     5221 atcggctttg gtcagcgcca ctgtcagcat cgggttaccg gtcagctgca aaatcgccag
     5281 atgctcacgg gtctgtgcca tcacgccgtc atcgcacgcc accaccaaca gcgcgtgatc
     5341 gataccacca acgcccgcca gcatgttgga aagaaacttt tcatgaccgg gaacgtcgat
     5401 aaaaccaggc acgcgaccat ccggctgcgg ccagtaggca tagccgagat cgatggtcat
     5461 gccgcgcttt ttttcttccg gcagacggtc agcatttacg ccagtaatcg cctgcaataa
     5521 ggttgttttg ccgtggtcaa cgtgtccggc agtcgcaata atcatttcaa caacatctcc
     5581 aaaaaccgtt gctcatcttc aaggcagcgt aaatccagcc acaatcgtcc gtcataaata
     5641 cgaccaatca ccggcactgg caattcacgc cagcgggcgg ctaatgactc aaggtggcta
     5701 ccgcgtccat catggggtgt aaacgttaat gccgcgctcg gcaggcgatc aaccggcagc
     5761 gaaccactgc caatctgcga aagacatggc ataacctgta ccgcaaactc cgcgccgtaa
     5821 tgtgcgacaa ggggagcctg taaacgttgt gcctggattt gaatgacctc tgcgctgcgg
     5881 gtaagcaggc gcagggtcgg taatttttca ctcagagctt cagggtgtaa ataaagacgc
     5941 aacgtggctt ccagcgccgc gagggtcatt ttatccgcgc gtaatgcacg cttcagcggg
     6001 tggctttgca ggcgggcgat catctctttt ttaccaacga taatcccggc ctgcggcccg
     6061 cctaacaact tgtcgccgga gaagctcacc agactgacgc ccgccgcaat caactcctgc
     6121 ggcattggct ctttcggcaa accgtactgg ctaagatcga ccagcgagcc actgcctaaa
     6181 tcagtcacca ctggaacatc cagctctttg ccgagcgcca ccagttcagc ttcgtctatc
     6241 gctttggtga acccctgaat gctgtagtta ctggtatgta ctttcatcaa cagtgcggta
     6301 ttttcattca ccgcctgacg ataatcattc gcgtgcgtgc ggttggtggt ccctacttcg
     6361 tgtagggtgc agcctgcctg acgcataaca tcgggaatac gaaacgcgcc gccaatctcc
     6421 accagttcgc cgcgagatac caccacctct ttcccgctgg cagtggccgc caacatcaat
     6481 aacaccgccg ccgcattgtt attgacgata caggcatctt ccgcccccgt aatacggcac
     6541 agcagctgcg ccagcgcccg atcgcgatgt ccgcgtccgg cgtcgtccag atcgtactcg
     6601 agggtcactg gcgaacgcat agcctgcgca acggcttcca ccgcggcttc cgcctgtaaa
     6661 gctcgcccaa ggttggtatg cagcacggtt cccgtcaggt tgatcaccgg acgcagcgcg
     6721 ctctgcgcct ctttcgtcaa ccgggcatcg acttcttgcg cccagttttc acaccacgca
     6781 ggcagcgtct ggctgccacg aatcacttct cgcgcttcgt cgagcatctg acgcaacaat
     6841 tccaccacgc gggtgtgacc ataagtatca cgcaaagaaa ggaaggagct atcgcgcaat
     6901 aagcgatcaa tagccggaag ttgactatag agggaacgcg tttcggttgt cataggaaac
     6961 ctggctgatc aaggccctct cacacggaga agggcgttta acataaccac ggattgtaac
     7021 gtgagatggg tcaggaggac atatcgcgca tcaagccttt ggcggttcgg tgcgagcaaa
     7081 actttcgcgg ctaaacaggt tttcgaccag tttgactaag tgtgggcggt cgacacacca
     7141 acctggcgca acgcggcgga aattgagata tccgacagca caggcaatgg cgatagtcgc
     7201 cagattgacc gtatcggttt tgagtgtgcc atcgaccaga tatccttcca gcacatccag
     7261 actgcggttg attttctccc gctggcgtaa caattcatct tcagactgct gcgccgccgg
     7321 acgcgcctgt tcacgcaccg ataccagccc ggcatccata atgccatccg ccagcgcctc
     7381 aattttgcgc acccgcaacg actccagcgg atcgcgcggc aacatcgccg gagcgacatt
     7441 cattaactca atatattcag cgatgatcgg cgaatcaaac cagcattcgc cctcttcggt
     7501 caccagcacc ggcactttcc ctaacgggtt aaattgcgcc acgccgttat ccgcgttata
     7561 gggcagttca ttaataaatt cgaaagttat gcccttttct aacaacagaa taaaaagttt
     7621 gcgtacaaac gggctggtgt agctaccgac gagtttcatg ccgagtcctt tgtgcgagga
     7681 aaaatatcag tatggctcag gctcacgcaa aacacaggtg atataagtct caatgatcca
     7741 aataaagata atgcatccaa ctggtcattc ttagcagcac tttccgcatc accgaaacat
     7801 ggctgaaatg gtcggagtaa accgccacct gggcgtagat gccgtcgggt aaggcatcga
     7861 tatcatcgtt agggtccagt tcaatggttc ccagcacacc gtccgtgccg ggaacgaccg
     7921 ttaatgattg caataccccc tgcgcctgat aagaaccgcc tggcacgaca ggtaaaatac
     7981 tagtcaattt gccgtggaac acctgcccag gtagcgcgtt aaacaccact tccgcatcat
     8041 cgccaggttt cagacgtaac agcgagtttt gccgaaattg ggcgacaatt tgccgttttt
     8101 gctcggggat gaagaccatc accggacgca gcggcaaggc agctgcgtat gtacctgggc
     8161 ggatcagtac ctgagtgacg tagccattgc ttggtgcgcg aatgacagtc tgctcaagat
     8221 tatattttgc ttcagtaagt tgcgctctta agctcacaat ctgcgattgc tcgccgttga
     8281 ccatactgtc gagctgactc tggatctgcg cctgctccgc caccgagcct ttcactaacg
     8341 catcctgcgc gaggaaattt tgccgcgcat cgtcgatgtc acgttccgag aacggattca
     8401 ccgccgcctg gctgcctttc aggtaacgtt gataattttt aaacagacgg tcgcgctccg
     8461 ctgaaacctg ggtggtgttg gcctgcgctt cagtgagctg cgcacgcagc gtctttatat
     8521 tatgcgtcgc cgtcatcagg tcagcctgaa gtctgtcaac tcgcgcctgg taacgaacag
     8581 ggtcgagctt aaaaagcacc tcgccctttt gaataagctg attattcttg tcagtgactt
     8641 cagtaacaat tcccgtcacc tgtggcgtga tagggatcgc tatcactgcc ttttgcgcgg
     8701 taaaagtgta agggtggttg tagttcatca acaaaatcaa accactcacc agaaacacgc
     8761 ctcccagcgc cgccgtcgcc agcgccacgt aagttaaaac aatcaataga tccataatta
     8821 gatctccggg aacgtggaat agtcagtctt tttctcggcg gcgagttgat gctccagccg
     8881 ggaaattcgg tcagaaagtg cggcgatttc cggttcagtc gctttctcct gcgacgcaac
     8941 atgtgactgc ataccccagc cacgctccgg ttgatagagc gtcgcccaga tccacagaaa
     9001 cggccagata acatgcagag taaacaggct cacccagccc gccgtatgaa tagcgtcggc
     9061 atgggggtga ttgcgctttt tggcaatcag ataagggatg tcatgtatcg cgatgattcc
     9121 ataaaaaatc accagaaaga cgaagatcag cactcccaac gcgaaatagt ttagaaacat
     9181 atctgcctcg gattcacgtt tatcagtgtt gttttttggc tggcagccag aagggagtca
     9241 ggctgatatt ttgacaataa tccgggttcg cgattctcgc cataacacca aagaataatt
     9301 tttagaggtg atgagttgct tagttacata acgattgtat gacgaaggca taacatgctg
     9361 tagatcacat caggtgaacg ccgtaagaaa atatcttgtg attcagatca caaagattca
     9421 acaaaccatc aaaacaaaaa tgtgacatta ctcacattta aatgccattt ttagcgaaaa
     9481 tcaccgcctt gtttcttttt tatacaggcg ttttgtgatg agcgtcacgt caattacctc
     9541 tctaccccct atatttatgt gactgatatc acacaaaagc ccgtcgactg gacagttaac
     9601 cgattcagtg ccagatttcg cagtatctac aaggtccggc tacctctgcc gccacattaa
     9661 caaaaaacct cgggcttcca gcctgcgcga cagcaaacat aagaaggggt gtttttatgt
     9721 catccgatat taagatcaaa gtgcaaagct ttggtcgttt cctcagcaac atggtgatgc
     9781 caaatatcgg cgcgtttatc gcgtggggta tcatcaccgc gttatttatt ccaacagggt
     9841 ggttaccgaa cgagacgctg gcgaagctgg tcgggccgat gatcacttat ctcctaccgc
     9901 tgctgatcgg ttataccggt ggtaagctgg taggcggcaa acgtggtggc gtagtcggtg
     9961 ccattaccac catgggcgtt atcgtcggcg cagacatgcc gatgttcctc ggttctatga
    10021 ttgcaggtcc gctgggcggc tggtgcatta agcacttcga ccgctgggta gacggtaaga
    10081 tcaaatccgg ttttgagatg ctggtgaata acttctccgc aggcatcatc gggatgatcc
    10141 tcgctattct ggcattcctc ggcattggcc cgattgttga agccctgtcc aaaatgctgg
    10201 ctgcgggcgt taacttcatg gttgtccatg acatgctgcc gctggcgtct atctttgttg
    10261 aaccggcgaa aatcctgttc ctcaacaacg ccattaacca cggtatcttc tcgccgctgg
    10321 gtattcagca gtcccatgaa ctgggtaaat caatcttctt cctgattgaa gctaacccag
    10381 gtccaggtat gggcgtgctg ctggcgtaca tgttctttgg tcgtggtagc gctaaacagt
    10441 ctgcgggcgg tgcggcaatc atccacttcc tgggtggtat ccacgaaatc tacttcccgt
    10501 atgtgctgat gaatccgcgt ctgatcctcg cagtcatcct cggcggtatg actggcgtgt
    10561 tcacgctgac tatcctgggc ggtggtctgg tttctccggc atctccgggt tctatccttg
    10621 ctgtactggc gatgacacca aaaggtgctt atttcgctaa catcgcgggt gtgtgtgcgg
    10681 cgatggctgt ctccttcgtt gtctctgcta ttttgctgaa aaccagcaaa gtgaaagaag
    10741 aagatgatat tgaagcagca actcgtcgta tgcaggacat gaaagctgag tctaaaggca
    10801 catctccgct atctgcgggc gatgtgacta acgacctgag ccacgtacgt aaaatcatcg
    10861 ttgcctgtga cgccggtatg ggttcaagtg cgatgggcgc aggcgtgctg cgtaagaaaa
    10921 ttcaggatgc aggtctgtcg cagatttcgg tcactaacag cgcgatcaac aacctgccgc
    10981 cagatgtgga cctcgtcatc actcaccgtg acctgaccga acgcgctatg cgccaggttc
    11041 cgcaggcaca gcatatttcg ctgaccaact tcctcgacag cggcctgtac accagcctaa
    11101 ccgaacgtct cgttgctgcc cagcgccata ctgaaaacga agtgaaagtg aaagacagtc
    11161 tgaaagacag ctttgacgat tccagtgcta acctgttcaa actgggcgcg gagaacatct
    11221 tcctcggtcg caaagcagca accaaagaag aagcgattcg ttttgctggc gagcagctgg
    11281 tgaaaggcgg ttacgttgag ccggaatacg ttcaggcgat gctggatcgt gaaaaactga
    11341 ccccgactta tctgggtgag tctatcgcgg tgccacacgg tacggttgaa gcgaaagatc
    11401 gcgtactgaa aacgggcgtg gtgttctgcc agtacccgga aggcgtgcgc ttcggtgaag
    11461 aagaagatga cattgcccgt ctggtgattg gtattgcagc ccgtaacaac gagcacattc
    11521 aggttatcac cagcctgacc aatgcactgg atgatgagtc cgtcatcgag cgtctggcac
    11581 acaccaccag cgtggatgaa gtgctggaac tgctggcagg tcgtaagtaa tccaatccta
    11641 ccctctcctc acggagaggg tttgggtgag ggaaaagcct caccccagcc ctctcgggta
    11701 aaaacattga tgaaggttaa tactatgaaa gcattacatt ttggcgcagg taatatcggt
    11761 cgtggcttta tcggtaaact gctggcagac gcgggtatcc aactgacgtt tgccgatgtc
    11821 aatcaggtgg tacttgatgc cctgaatgcc cgtcatagct atcaggttca tgtggtcggt
    11881 gaaaccgagc aggtagatac cgtttccggc gtcaatgctg tcagcagcat tggtgatgat
    11941 gtcgttgatc tgattgctca ggtcgattta gtcactaccg ccgttggccc ggttgtgctg
    12001 gaacgtattg ctccggctat cgccaaaggg ctggtgaaac gcaaagaaca aggtaatgaa
    12061 tccccgctga acatcatcgc ctgtgaaaac atggtacgcg gcaccacgca gctgaaaggt
    12121 catgtgatga acgccctgcc ggaagacgcc aaagcgtggg tagaagagca cgttggcttt
    12181 gtcgattccg ccgttgaccg catcgtaccg ccttcggctt cggcaactaa cgatccgctg
    12241 gaagtgacgg tagaaacttt cagcgaatgg attgtcgata aaacgcagtt caaaggcgca
    12301 ctgccgaaca tcccaggcat ggagttaacc gacaacctga tggcatttgt cgaacgtaaa
    12361 ctcttcaccc tgaacacggg tcatgctata accgcgtacc tcggaaaact ggccggtcat
    12421 cagaccattc gtgacgctat tctcgacgag aaaatccgcg cggtggtaaa aggtgcgatg
    12481 gaagaaagtg gtgcggtact gatcaagcgc tacggctttg acgcagacaa acatgcggcg
    12541 tacatccaga aaatcctcgg tcgttttgag aacccgtatc tgaaagatga tgtagagcgc
    12601 gtaggccgtc agccgctgcg taaactgagt gctggcgacc gtctgatcaa gccactgctc
    12661 ggtacgctgg aatacagcct tccgcacaag aatctgattc aggggattgc tggtgcaatg
    12721 cacttccgca gtgaagatga tccgcaggct caggaactgg cagcactgat cgctgacaaa
    12781 ggtccgcagg cggcgctggc acagatttcc ggtcttgatg ccaacagcga ggttgtatcc
    12841 gaggcggtaa ccgcttataa agcaatgcaa taatggtgga ccaggcgcag gacaccctgc
    12901 gcccgaataa cagattgtca gatatgcagg caacaatgga acaaacccag gccgttgaaa
    12961 accgtgtgct tgagcgtctg aatgctggca aaaccgtgcg aagctttctg atcaccgccg
    13021 tcgagctttt gaccgaggcg gtaaatcttc tggtgcttca ggtattccgc aaagacgatt
    13081 acgcggtgaa gtatgctgta gaaccgttac tcgacggcga cggtccgctg ggcgatcttt
    13141 ctgtgcgttt aaaactcatt tacgggttgg gcgtcattaa ccgccaggaa tacgaagatg
    13201 cggaactgct gatggcattg cgtgaagagc taaatcacga cggcaacgag tacgccttta
    13261 ccgacgacga aatccttgga ccctttggtg aactgcactg cgtggcggcg ttaccaccgc
    13321 cgccacagtt tgaaccagca gactccagtt tgtatgcaat gcaaattcag cgctatcaac
    13381 aggctgtgcg atcaacaatg gtcctttcac tgactgagct gatttccaaa atcagcttaa
    13441 aaaaagcctt tcaaaagtaa gcaacgtctg cttactgccc ctctacctgc ttcggccgat
    13501 aaagccgacg ataatactcc agacgttgta gatataacgg cacgctttca tcgggtattc
    13561 cggacggaat cgcgttacgg ggaggaagtt ttttcagata ctcccggaac gcctggcttg
    13621 atgccatgaa atctacggct ttatcgataa gaagcggaac gttttcacct aatttgccca
    13681 tgatgatatc tccgtattac cccgcgccgg gaatgcgcgg ccgccaattt tagtttaggt
    13741 tcccggtaaa gatgacatta aggaaaacgt gctgaatcct caataatcat ccgctgctgt
    13801 ttattatctt tttctataga tttcttaatt aatcaacgaa ttgcgtagtt tttgagcatg
    13861 ttaatgatta cgttgcagaa ctattacaat gtcatcagtt gtgaacaaag cacctggtcg
    13921 cgcatactag gggctataaa tttatcttta tcagaagcca tcacatgaaa gaagtcgaaa
    13981 aaaacgaaat caagcgtctc agcgatcgcc tggacgccat ccgccaccag caggccgatc
    14041 tgtcgctggt tgaagccgca gacaaatatg ccgagctgga aaaagagaaa gccacgctgg
    14101 aagcagaaat tgctcgcctg cgtgaggttc atagccaaaa actgagtaaa gaagcacaga
    14161 agctgatgaa gatgccgttc cagcgcgcaa ttaccaaaaa agagcaggct gatatgggca
    14221 agctgaagaa aagtgttcgc ggactggtgg ttgtgcaccc aatgaccgcg ctggggcgcg
    14281 aaatgggcct ggaagagat
//
LOCUS       DAJTRC010000092        14181 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.092, whole genome shotgun sequence.
ACCESSION   DAJTRC010000092 DAJTRC010000000
VERSION     DAJTRC010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 14181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.092"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..300
                     /locus_tag="OO357_003104"
     CDS             <1..300
                     /locus_tag="OO357_003104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5887962.1"
                     /translation="GVTSSMLKISCPKEVAGVKLSLITNDKITGNDKAIASSNDTVGD
                     NSDVLDVSAPFNIESYKTAEGQYAIPFKAKYLKLTDNSVQSGDVLSSLVMRVAQD"
     gene            381..1055
                     /locus_tag="OO357_003105"
     CDS             381..1055
                     /locus_tag="OO357_003105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5887963.1"
                     /translation="MKGLLSLLIFSMVLPAHAGIVIYGTRIIYPAENKEVMVQLMNQG
                     NRSSLLQAWIDDGDTSLPPEKIQVPFMLTPPVAKIGANSGQQVKIKIMPNKLPTNKES
                     IFYLNVLYIPPNSPEQEGKNALKFAMQNRIKLFYRPAGIAPVNKATFKKLLVNRSGNG
                     LVIKNDSANWVTISDVKANNVKVNYETIMIAPLESQSVNIKSNNANNWYLTIINDHGN
                     YISDKI"
     gene            1071..3533
                     /locus_tag="OO357_003106"
     CDS             1071..3533
                     /locus_tag="OO357_003106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707994.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5887964.1"
                     /translation="MLRMTPLASAIVALLLGIEAYAAEETFDTHFMIGGMKDQQVANI
                     RLDDNQPLPGQYDIDIYVNKQWRGKYEIIVKDNPQETCLSREVIKRLGINSDNFASGK
                     QCLTFEQLVQGGSYSWDIGVFRLDFSVPQAWVEELESGYVPPENWERGINAFYTSYYV
                     SQYYSDYKASGNNKSTYVRFNSGLNLLEWQLHSDASFSKTNNNPGVWKSNTLYLERGF
                     AQFLGTLRVGDMYTSSDIFDSVRFSGVRLFRDMQMLPNSKQNFTPRVQGIAQSNALVT
                     IEQNGFVVYQKEVPPGPFAITDLQLAGGGADLDVSVKEADGSVTTYLVPYAAVPNMLQ
                     PGVSKYDFAAGASKQSDFVQAGYQYGFNNLLTLYGGTMVANNYYAFTLGTGWNTRIGA
                     ISVDATKSHSKQDNGDVFDGQSYQIAYNKFVSQTSTRFGLAAWRYSSRDYRTFNDHVW
                     ANNKDNYRRDENDIYDIADYYQNDFGRKNSFSANMSQSLPEGWGSVSLSTLWRDYWGR
                     SGSSKDYQLSYSNNWRRISYTLAASQAYGENHHEEKRFNIFISIPCDWGDDVTTPRRQ
                     IYMSNSTTFDDQGFASNNTGLSGTVGSRDQFNYGVNLSHQHQGNETTAGANLTWNAPV
                     ATVNGSYSQSSTYRQTGASVSGGIVAWSGGVNLANRLSETFAVMNAPGIKDAYVNGQK
                     YRTTNRNGVVVYDGMTPYRENHLMLDVSQSDSEAELRGNRKIAAPYRGAVVLVNFDTD
                     QRKPWFIKALRADGQPLTFGYEVNDIHGHNIGVVGQGSQLFIRTNEIPPSVNVAIDKQ
                     QGLSCTITFGKEIDESRNYICQ"
     gene            3549..4582
                     /locus_tag="OO357_003107"
                     /pseudo
     CDS             3549..4582
                     /locus_tag="OO357_003107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416611.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="putative fimbrial-like adhesin protein"
     gene            complement(4664..5002)
                     /gene="rcnB"
                     /locus_tag="OO357_003108"
     CDS             complement(4664..5002)
                     /gene="rcnB"
                     /locus_tag="OO357_003108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II) efflux transporter accessory
                     subunit RcnB"
                     /protein_id="HCR5887965.1"
                     /translation="MTIKNKMLLGVLLLVTSAAWAAPATAGSTNTSGISKYELSSFIA
                     DFKHFKPGDTVPEMYRTDEYNIKQWQLRNLPAPDAGTHWTYMGGAYVLISDTDGKIIK
                     AYDGEIFYHR"
     gene            complement(5221..6051)
                     /gene="rcnA"
                     /locus_tag="OO357_003109"
                     /pseudo
     CDS             complement(5221..6051)
                     /gene="rcnA"
                     /locus_tag="OO357_003109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416609.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt efflux protein RcnA"
     gene            6173..6445
                     /gene="rcnR"
                     /locus_tag="OO357_003110"
     CDS             6173..6445
                     /gene="rcnR"
                     /locus_tag="OO357_003110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ni(II)/Co(II)-binding transcriptional repressor
                     RcnR"
                     /protein_id="HCR5887966.1"
                     /translation="MSHTIRDKQKLKARASKIQGQVVALKKMLDEPHECAAVLQQIAA
                     IRGAVNGLMREVIKGHLTEHIVHQGDELKREEDLDVVLKVLDSYIK"
     regulatory      6537..6633
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            6668..7456
                     /gene="thiM"
                     /locus_tag="OO357_003111"
     CDS             6668..7456
                     /gene="thiM"
                     /locus_tag="OO357_003111"
                     /EC_number="2.7.1.50"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyethylthiazole kinase"
                     /protein_id="HCR5887967.1"
                     /translation="MQVDLLSSAQSAHALHLFHQHSPLVHCMTNDVVQTFTANTLLAL
                     GASPAMVIETEEASQFAAIASALLINVGTLTQPRAQAMRAAVEQAKSSQTRWTLDPVA
                     VGALDYRRHFCHELLSFKPAAIRGNASEIMALAGIANGGRGVDTTDAAANAIPAAQTL
                     ARETGAIVVVTGEVDYVTDGHRIIGIHGGDPLMTKVVGTGCALSAVVAACCALPGDTL
                     ENVASACHWMKQAGERAVARSEGPGSFVPHFLDALWQLTQEVQA"
     gene            7453..8253
                     /gene="thiD"
                     /locus_tag="OO357_003112"
     CDS             7453..8253
                     /gene="thiD"
                     /locus_tag="OO357_003112"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HCR5887968.1"
                     /translation="MKRINALTIAGTDPSGGAGIQADLKTFSALGAYGCSVITALVAQ
                     NTRGVQSVYRIEPDFVAAQLDSVFSDVRIDTTKIGMLAETDIVEAVAERLQRYQIQNV
                     VLDTVMLAKSGDPLLSPSAVATLRSRLLPQVSLITPNLPEAAALLDAPHARTEQEMLE
                     QGRSLLAMGCGAVLMKGGHLDDEQSPDWLFTREGEQRFTAPRIMTKNTHGTGCTLSAA
                     LAALRPRHTNWADTVQEAKIWLSSALAQADTLEVGHGIGPVHHFHAWW"
     gene            8318..9139
                     /locus_tag="OO357_003113"
     CDS             8318..9139
                     /locus_tag="OO357_003113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HCR5887969.1"
                     /translation="MQLRITSRKKLTALLCALGLISIVAIYPRQTVNFFYSTAVQITD
                     YIHFYGYRPVKSFAIRIPASYTIHGLDVSRWQERIDWQRVAKMRDNDIRLQFAFIKAT
                     EGEKLVDPYFSRNWQLSRENGLLRGAYHYFSPSVSASVQARLFLQTVDFSQGDFPAVL
                     DVEERGKLSAKELRKRVSQWLKMVEKRTGKKPIIYSGAVFYHTNLAGYFNEYPWWVAH
                     YYQRRPDNDGMAWRFWQHSDRGQVDGINGPVDFNVFNGTGMSCRHSLMGLKKRLK"
     gene            9187..9933
                     /locus_tag="OO357_003114"
     CDS             9187..9933
                     /locus_tag="OO357_003114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5887970.1"
                     /translation="MEQAHTQLIAQLNERILAADNTPLYIKFAETVKNAVRSGVLEHG
                     NILPGERDLSQLTGVSRITVRKAMQALEEEGVVTRSRGYGTQINNIFEYSLKEARGFS
                     QQVVLRGKKPDTLWVNKRVVKCPEEVAQQLAAEAGSDVFLLKRIRYVDEEAVSIEESW
                     VPAHLIHDVDAIGISLYDYFRSQHIYPQRTRSRVSARMPDAEFQSHIQLDSKIPVLVI
                     KQVALDQQQRPIEYSISHCRSDLYVFVCEE"
     gene            complement(9907..10872)
                     /locus_tag="OO357_003115"
     CDS             complement(9907..10872)
                     /locus_tag="OO357_003115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase"
                     /protein_id="HCR5887971.1"
                     /translation="MSGARLHTLLPELTTRQPVMVVGAAVIDVIADAYALPWRGCDIE
                     LKQQSVNVGGCALNIAVALKRLGIEAGNALPLGQGVWAEIIRNRMAKEGLISLIDNAE
                     SDNGWCLALVEPDGERTFMSFSGVENQWNRQWLARLTVAPGNLLYFSGYQLASPCGEL
                     LVEWLEKLQDVTPFIDFCPRIGDIPDALLARIMACRPLVSLNRQEAEIAAERFALSAE
                     ITTLGKQWQEKFAAPLIVRLDKEGAWYFSNDASGCIPAFPTQVVDTIGAGDSHAGGVL
                     AGLASGLPLADAVLLGNAVASWVVGHRGGDCAPTREELLLAHKNV"
     gene            complement(10869..11872)
                     /locus_tag="OO357_003116"
                     /pseudo
     CDS             complement(10869..11872)
                     /locus_tag="OO357_003116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
     gene            11912..12964
                     /gene="fbaB"
                     /locus_tag="OO357_003117"
     CDS             11912..12964
                     /gene="fbaB"
                     /locus_tag="OO357_003117"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006820796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I fructose-bisphosphate aldolase"
                     /protein_id="HCR5887972.1"
                     /translation="MTDIAQLLGKDADNLLQHRCMTIPSDQLYLPGHDYVDRVMIDNN
                     RPPAVLRNMQTLYNTGRLAGTGYLSILPVDQGVEHSAGASFAANPLYFDPKNIVELAI
                     EAGCNCVASTYGVLVSVSRRYAHRIPFLVKLNHNETLSYPNTYDQTLYASVEQAFNMG
                     AVAVGATIYFGSEESRRQIEEISAAFERAHELGMVTVLWAYLRNSAFKKDGVDYHVSA
                     DLTGQANHLAATIGADIVKQKMAENNGGYKAINYGYTDDRVYSKLTSENPIDLVRYQL
                     ANCYMGRAGLINSGGAAGGETDLSDAVRTAVINKRAGGMGLILGRKAFKKSMADGVKL
                     INAVQDVYLDSKITIA"
     gene            13272..14126
                     /gene="gatY"
                     /locus_tag="OO357_003118"
     CDS             13272..14126
                     /gene="gatY"
                     /locus_tag="OO357_003118"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatY"
                     /protein_id="HCR5887973.1"
                     /translation="MYVVSTKQMLNNAQRGGYAVPAFNIHNLETMQVVVETAANLHAP
                     VIIAGTPGTFTHAGTENLLALVSAMAKQYHHPLAIHLDHHTKFDDIAQKVRSGVRSVM
                     IDASHLPFAQNISRVKEVVDFCHRFDVSVEAELGQLGGQEDDVQVNEVDALYTNPAQA
                     REFAEATGIDSLAVAIGTAHGMYASAPVLDFSRLENIRQWVNLPLVLHGASGLSTKDI
                     QQTIKLGICKINVATELKNAFLQSLKNYLTEHPEATDPRDYLQSAKSAMRDVVSKVIA
                     DCGCEGRA"
BASE COUNT         3751 a         3302 c         3593 g         3535 t
ORIGIN      
        1 ggtgtaacca gcagcatgtt aaaaattagc tgcccgaaag aagttgctgg tgtaaaactc
       61 tcgttgatca caaacgacaa aataaccggt aacgataagg cgatagccag tagcaacgat
      121 accgtaggtg ataacagcga tgtcctagat gtttctgcac cttttaacat tgagagttat
      181 aaaacagcgg aaggtcaata tgctattccg tttaaagcaa aatacctgaa actgacagat
      241 aactcagtgc aatcaggtga tgtgttatct tctctggtta tgcgtgtggc gcaggattaa
      301 taaataaaat tgcaagtttc tccaggtaat aaacatggcc gctatcccca tggcggcttt
      361 ttaatttaag aggcattaag atgaaaggat tattatcttt actcattttt tctatggtcc
      421 ttcctgcaca tgccggaatt gttatctacg ggacgcgcat tatttacccg gcagaaaata
      481 aagaagttat ggtgcagttg atgaaccagg gaaatcgttc ttcgctgctg caggcgtgga
      541 ttgatgatgg cgatacgtcg ttaccaccag aaaaaattca ggttcctttc atgttaacgc
      601 caccagtggc aaaaataggg gcaaattccg ggcagcaagt aaaaatcaaa attatgccga
      661 ataaactgcc cactaataaa gaaagcattt tttatctgaa tgttctgtat attccaccaa
      721 atagtccaga gcaagaaggc aagaatgcac tgaagtttgc gatgcaaaac agaattaagt
      781 tgttttaccg gccagcgggt attgctccgg taaataaagc gacctttaaa aagttactgg
      841 taaatcgcag tggcaatgga ttggtgataa aaaatgactc agctaattgg gtgacgattt
      901 cggatgtcaa agctaataat gttaaagtca attatgaaac tattatgatt gcccccttag
      961 aaagtcagag tgttaatatc aaaagtaata atgcaaataa ctggtatctg accattatca
     1021 atgaccacgg caactatatt agtgacaaaa tttaattgca ggagctgcct atgttgagaa
     1081 tgaccccact tgcatcagca atcgtagcgt tattgctcgg cattgaagct tatgcagctg
     1141 aagaaacctt tgatacccat tttatgatag gtggaatgaa agaccagcag gttgcaaata
     1201 ttcgtcttga tgataatcaa cccttaccgg ggcagtatga catcgatatt tatgtcaata
     1261 agcaatggcg cgggaaatat gagattattg ttaaagacaa cccgcaagaa acatgtttat
     1321 caagagaagt tatcaagcgg ttaggcatta atagcgataa cttcgccagc ggtaagcaat
     1381 gtttaacatt tgagcaactt gttcagggtg ggagctatag ctgggatatc ggggtttttc
     1441 gtctcgattt cagtgtcccg caggcttggg tggaagaact ggaaagtggc tatgttccac
     1501 cggaaaactg ggagcggggt attaatgcgt tttatacttc ttattatgtg agtcagtatt
     1561 acagcgacta taaagcgtcg ggtaacaaca agagtacata tgtacgtttt aacagcgggt
     1621 taaatttact ggagtggcaa ctgcattctg atgccagttt cagtaaaaca aataacaatc
     1681 caggggtgtg gaaaagcaat accctgtatc tggaacgtgg atttgcccaa tttctcggca
     1741 cgcttcgtgt gggtgatatg tacacatcaa gcgatatttt tgattctgtt cgcttcagcg
     1801 gtgtgcggtt gtttcgtgat atgcagatgt tgcctaactc gaaacaaaat tttacgccac
     1861 gggtgcaggg gattgctcag agtaacgcgc tggtaactat tgaacagaat ggttttgtgg
     1921 tttatcagaa agaggttcct cctggcccgt ttgcgattac agatttgcag ttggccggtg
     1981 gtggagcaga tcttgatgtt agcgtgaaag aggcggacgg ctcggtaacc acctatctgg
     2041 tgccttatgc agcggtgcca aatatgctgc aacccggcgt gtcgaaatat gattttgcgg
     2101 cgggggcgag caagcaaagt gattttgtcc aggcaggtta tcagtatggt tttaataact
     2161 tattgacgct gtatggcggc acgatggttg ctaataatta ctatgcgttt accctcggaa
     2221 cgggttggaa cacgcgcatt ggcgcaattt ccgtcgatgc cacgaagtcg catagtaaac
     2281 aagacaacgg cgatgtgttt gacgggcaaa gttatcaaat tgcctacaac aaatttgtga
     2341 gccaaacgtc gacgcgtttt ggtctggcgg cctggcgtta ttcgtcgcgt gattaccgga
     2401 catttaacga tcacgtttgg gcaaacaata aagataatta tcgccgtgat gaaaacgata
     2461 tctatgacat tgccgattat taccagaacg attttggccg taaaaatagc ttttctgcca
     2521 atatgagcca gtcattgcca gaaggctggg gttctgtgtc cttaagtacg ttatggcgag
     2581 attactgggg gcgtagcggc agcagtaagg attatcagtt gagttattcc aataactggc
     2641 ggcggataag ctataccctc gcggcaagcc aggcgtatgg cgagaatcat catgaagaga
     2701 aacgttttaa tatttttata tcaattccct gtgattgggg tgatgacgtt acgacgcctc
     2761 gtcggcaaat atatatgtct aactcaacga cgtttgatga tcagggtttt gcctcaaata
     2821 atacgggatt atcaggaaca gtagggagtc gggatcagtt caattatggt gtcaacctga
     2881 gtcatcaaca tcagggaaat gaaacgacag ctggggcgaa tttgacctgg aacgcgccgg
     2941 ttgcgacagt gaatggcagt tatagtcagt cgagtactta tcgacagact ggagccagtg
     3001 tttcaggggg cattgtcgcc tggtcgggtg gcgttaatct ggcgaaccgt ctttccgaaa
     3061 cgtttgctgt gatgaatgcg ccaggaatta aagatgctta tgtcaatggg caaaaatatc
     3121 gcacaacaaa ccgtaatgga gtggtggtat acgacggaat gacaccttat cgggaaaatc
     3181 acctgatgct ggatgtgtcg caaagcgata gcgaagcaga attacgtggc aaccggaaaa
     3241 ttgccgcccc ttatcgcggc gcggttgtac tggttaattt tgataccgat cagcgcaagc
     3301 cctggtttat aaaagcgtta agagcggatg gtcaaccatt aacgtttggt tatgaagtca
     3361 atgatatcca tggtcataat attggcgttg tcggccaggg aagccagtta tttattcgca
     3421 ccaatgaaat accgccatcg gttaatgtag caattgataa gcaacaagga ctttcatgca
     3481 caatcacctt cggtaaagag attgatgaaa gtagaaatta tatttgccag taatagtcag
     3541 gtggttctat ggaaattcgc ataatgctat ttatattaat gatgatggtt atgcctgtga
     3601 gctatgcggc atgttatagt gagttatctg ttcagcacaa tttggttgtt caggggaatt
     3661 ttgcacttac tcaaacacaa atagcgacat atgagcataa ttttaatgat tcgtcatgcg
     3721 tgagtacaaa tactatcacc cctatgagcc catcggatat tattgttgga ctttataacg
     3781 ataccattaa attaaattta cattttgaat ggaccaataa aaacaacatc acgttgtcaa
     3841 ataatcagag cagtttcacc agtggttatt cggtaacggt gacacctgcg gccagtaatg
     3901 caaaagtgaa tatttctgcg gggggcggtg gttcagtgat gattaatggt gttgcgacat
     3961 tatccagtgc ttcatcatcg acacgcggga gtgccgcagt acaatttcta ctgtgtttat
     4021 taggtggcaa gtcatggggt gcatgtgtaa atagctacag aaatgcattg gcacaaaatg
     4081 caggtgtcta ttccttcaat ctgacattgt catacaaccc gataaccaca acctgcaaac
     4141 aggacgattt attaattact ttagacagta ttcccgtttt acaattacca gccacaggta
     4201 acaaagcaac aataaatagt aaaaaaaggg atattattct gcgttgtaaa aatttattag
     4261 gtcaacaaaa tcaaacatca cggaaaatgc aggtgtattt atcaagttct gacttgttaa
     4321 ccaacagcaa cacaatactg aaaggtgcgg aagataatgc gtaggattta ttcttgaaag
     4381 taatggttcg ccagtcacac ttttaaatat cactaacagc agtaaaggat acacaaattt
     4441 aaaagaaatt gcggcaaagt caaaacttac agatacaacg gtttcaattc cgataacagc
     4501 cagttactac gtctacgata caaacaaagt taaatctggc gcactggagg caaccgcatt
     4561 aatcaacgtg aaatacgact aataacaagc aaaacgagca agtggctaat aataaaaaat
     4621 aacaaggtgt ctgcatttcc ccctcatgat gagggggctt tttttagcga tgataaaaaa
     4681 tctcaccgtc gtaggcttta atgattttac cgtcggtgtc gctgatcaac acgtacgcgc
     4741 cacccatata ggtccagtgc gtcccggcat caggcgcggg caggttacgt aactgccact
     4801 gcttaatgtt gtactcatcg gtgcggtaca tctctgggac ggtatcacct ggtttgaaat
     4861 gcttaaagtc agcaatgaaa ctacttaact catacttaga aattcccgag gtattggtcg
     4921 aacccgcggt ggctggtgcg gcccaggcgg cactggtaac cagcaaaagc acacccagca
     4981 acatcttatt tttaatggtc ataaactctc cactttgggg atctgtaata tcagtcgcat
     5041 atctttcctg tcatacaata taactgacaa gcgatatttt gttagtgatc ccatgatttc
     5101 gtaaaaagtg taaatgaaag gatgccctaa aagcatcctt agcgagcaga gagtcagcgg
     5161 aaatagctga tttaagaatc atcctgggct gaagataaca tgcaagccta aaggattgag
     5221 ttatcgcatt atgcccatga agccatgtac gcccatatac acaccgacta agccaatcaa
     5281 cagactggaa aaataggggg cgcgtttagc gagagtgtta aatccgctcc agcgttttgc
     5341 gacctgctga acgctgattg ctgcgccaac gcctacggtg acaagcgtta acgccaggcc
     5401 aatgctgaaa ctgacgacca gtgttgcgcc cagtgtcagg gctttcaact gaatgcatat
     5461 caacagcacg gtaattgctg ccgggcaggg gataaagcca ccggttaagc caaataacaa
     5521 aatttgccag ttgatgacct ctctaccatc aaagcgtcgt ttaatgtcat tggcatgggc
     5581 tcgtgcatgg gcatcctgat actcgccatg ttcgtgatgg tgatgatgtc cttggtcgtg
     5641 gtgatgttcg tgatcgtgat gatggtgatg atgctcataa tcatgcccgt gcatattctc
     5701 cagccagttg cgttcgccgc gctaggtacg ccagaacatc cagaacgcgg tgccaataat
     5761 gatcactgcg gaaatcagct ggagccacgg ttctgctgat tgagcagtaa agcgcttact
     5821 gatcaccatc ccgccaaagg caatcaacca gaccaccgcg gtatgcgaaa tggttgctgc
     5881 cagtccgagc atcaccgcct gtttaatggt gcctttgatg gcgatgataa acgccgccat
     5941 catcgttttt gagtgccctg gttccaggcc atgaagcgca ccaagtaaga tggcgctggg
     6001 gatgaagaac caggcgtttc cttgctgaag aagagttgta aattcggtca tgataataat
     6061 tcttagtatt aattcggcaa tctgattcta ctccccccca gtacctgata ctaccccccc
     6121 agtagattaa tagtgctatg atttttcata ttcttgttaa ccaggtgttg ccatgtctca
     6181 tacaatccgt gataaacaga aactgaaagc gcgtgccagt aagattcagg gccaggtcgt
     6241 ggcgctcaag aaaatgctcg acgagccgca cgaatgcgct gcagttttac aacagattgc
     6301 tgctatccgt ggagcggtaa acggtctgat gcgggaagtg attaaaggtc atctgacgga
     6361 acacatcgtt caccaggggg atgagctaaa acgtgaagaa gatctggatg tcgttctgaa
     6421 ggtgctggat tcatatatca aataatttat taacgcgatt gtaaaactgc cgtttttcct
     6481 cgtttacaac gggggcgctg gacattacca tcctcctctg cgatttatca tcgcaaccaa
     6541 acgactcggg gtgcccttct gcgtgaaggc tgagaaatac ccgtatcacc tgatctggat
     6601 aatgccagcg tagggaagtc acggaccacc aggtcattgc ttcttcacgt tatggcagga
     6661 gcaaactatg caagtcgacc tgctgagttc agcgcaatct gcgcacgcgt tacacctttt
     6721 tcaccaacat tcccctcttg tgcactgcat gaccaatgat gtggtgcaaa cctttaccgc
     6781 caataccttg ctggcgctcg gtgcatcgcc agcgatggtt atcgaaaccg aagaggccag
     6841 tcagtttgcg gctatcgcca gtgccttgtt gattaacgtt ggcacactga cgcagccacg
     6901 cgcacaggcg atgcgtgctg ccgttgagca agcaaaaagc tctcaaacac gctggacgct
     6961 tgatccagta gcggttggtg cgctcgatta tcgccgccat ttttgtcatg aacttttatc
     7021 ttttaaaccg gcagcgatac gtggtaatgc ttcggaaatc atggcattag ctggcattgc
     7081 taatggcgga cggggagtgg ataccactga cgccgcagct aacgcgatac ccgctgcaca
     7141 aacactggca cgggaaactg gcgcaatcgt cgtggtcact ggcgaggtgg attatgttac
     7201 cgatggacat cgtatcattg gtattcacgg tggcgatccg ttaatgacca aagtggtagg
     7261 aactggctgt gcattatcgg cggttgtcgc tgcctgctgt gcgttaccag gcgatacgct
     7321 ggaaaatgtc gcatctgcct gtcactggat gaaacaagcc ggagaacgcg cagtcgccag
     7381 aagcgagggg ccaggcagtt ttgttccaca tttccttgat gcgctctggc aattgacgca
     7441 ggaggtgcag gcatgaaacg aattaacgct ctgacgattg ccggtactga tccgagtggt
     7501 ggtgcgggga ttcaggccga tcttaaaacc ttctcggcac ttggcgctta tggttgctca
     7561 gttattactg cactggtggc gcaaaatacc cgtggcgtac agtcggtgta tcgcattgag
     7621 cctgattttg tcgccgccca gctcgattcg gtgttcagcg atgtgcgaat cgataccact
     7681 aaaatcggta tgttggcgga aaccgatatt gttgaagcgg tggcagaacg gttgcaacgt
     7741 tatcagatcc aaaacgtggt actcgacacc gttatgctgg caaaaagcgg cgacccgctg
     7801 ctttcacctt cggcggttgc tacgctgcgc agtcgattat tgccacaggt ttcattaata
     7861 acgccaaact tgcccgaagc tgccgccttg ctcgacgcgc cacacgcgcg caccgaacag
     7921 gaaatgctgg aacaagggcg atcgctgttg gcgatgggct gtggcgcagt gctaatgaaa
     7981 ggtggtcatc tggatgatga gcaaagcccg gactggctgt ttacccgcga gggtgaacaa
     8041 cggtttaccg caccgcgcat tatgaccaaa aacacccacg gcactggttg tacactctct
     8101 gcggcgttgg ctgcactacg cccgcgccat acaaactggg ctgacaccgt acaggaggca
     8161 aaaatctggc tttcatcggc gttagcccag gccgacacgc tggaagttgg tcacggtatt
     8221 ggtccggttc accacttcca cgcctggtgg tgagcaccgt actatagctg ctttggttat
     8281 tgtcagcctt tggcactgac attcagggat gaaaccaatg cagctaagaa ttaccagtcg
     8341 aaaaaaactc accgctctat tatgcgctct tgggctaatt tccatcgttg caatttatcc
     8401 gcgtcagacg gtgaattttt tctattcgac agcagttcag attacagact acatccactt
     8461 ctacggttat cgcccggtta aatctttcgc tattcgcatt cctgccagtt acaccattca
     8521 cggattagat gtttcgcgct ggcaggagcg tatcgactgg cagcgggtgg caaaaatgcg
     8581 cgacaacgat atccgcttac agtttgcttt tattaaggct acggaaggcg aaaagctggt
     8641 ggacccctat ttttcgcgta actggcaatt aagccgcgaa aatggcctgc tgcgcggggc
     8701 gtatcattat ttctccccgt cggtatccgc ttcagttcag gcgcgattat ttctgcaaac
     8761 ggtggatttc tcacaaggcg atttccctgc cgtgctggac gtagaagaac ggggaaaatt
     8821 atcggcaaaa gaattacgca agcgggtaag tcagtggcta aaaatggtcg aaaaacgcac
     8881 gggaaaaaag ccgattattt actcaggagc cgttttttat cacaccaatc tggcgggcta
     8941 tttcaatgaa tatccgtggt gggtggctca ctattatcaa cgtcgtccgg acaatgacgg
     9001 catggcctgg cgcttctggc agcattccga ccgtggacag gtagatggca tcaatggtcc
     9061 ggtggatttt aatgtgttta atggcacggg gatgagttgc aggcattcgt tgatgggatt
     9121 aaagaaacgc cttaaataag aagcagtgat atactcccac cagactccct ggcaggaaaa
     9181 agacaaatgg aacaagcgca tacccagctg attgcccaac tgaacgagcg tattttagcg
     9241 gcggataaca cgccgcttta tattaagttt gccgaaacgg taaaaaatgc cgtgcgcagc
     9301 ggggtgctgg agcatggcaa tattttgccc ggtgagcgtg atttaagtca gttaaccggc
     9361 gtttcgcgca ttacggtgcg caaggcgatg caggcgctgg aagaagaggg cgtggtgacg
     9421 cgttcgcgcg gttacggcac gcagatcaac aacatcttcg aatactcgtt gaaagaagcg
     9481 agaggttttt ctcagcaggt ggtattgcgc gggaaaaagc ccgatacgct atgggttaat
     9541 aagcgtgtcg tgaaatgtcc cgaagaagtt gcgcagcagc tggcggccga agcgggaagt
     9601 gatgtctttt tgcttaagcg tattcgctat gtcgatgaag aagcggtatc gattgaggaa
     9661 tcgtgggttc cggcgcattt aattcatgat gttgatgcca tcggaatttc tctttatgac
     9721 tatttccgca gccagcacat ttacccacaa cgtacgcgtt cccgcgttag cgcccggatg
     9781 ccggatgccg agtttcagtc acatattcag ttagatagca aaataccggt gctggtgatc
     9841 aagcaagtgg cgcttgacca acagcaacgg cctattgagt acagcattag ccactgtcgc
     9901 agcgatctat acgtttttgt gtgcgaggag tagttcctcg cgcgttggcg cacaatcacc
     9961 gccccgatgc ccgacaaccc acgacgccac tgcattgccc agtaatacgg catccgccag
    10021 tggcagacca gaggccagcc cggcaagtac gccacctgca tgactgtcgc ccgccccaat
    10081 ggtgtctaca acttgcgttg gaaatgccgg aatgcagcca gaagcgtcgt tgctgaaata
    10141 ccatgcgcct tctttatcga gacgaacgat caacggcgcg gcaaatttct cctgccattg
    10201 cttgccaagt gttgttatct ctgcggataa agcaaaacgt tcggcggcaa tctcagcctc
    10261 ttgacgattg agtgacacta aaggtcgaca ggccatgatc cgcgccagta atgcgtccgg
    10321 gatatcgcca atacgtgggc aaaaatcgat aaacggcgtc acgtcttgca gcttttccag
    10381 ccattccact aacaattcgc cgcagggcga ggccagttga taaccggaaa aatagagcag
    10441 gttgccaggc gcaacggtta atcgtgccaa ccattggcga ttccattgat tttccacacc
    10501 gctgaatgac ataaaagtgc gttcgccatc cggctcaacc agtgccagac accagccgtt
    10561 atcgctttcg gcgttatcga tcaggctgat taacccctct ttcgccatcc ggttgcgaat
    10621 aatctccgcc cacacgccct gaccgagcgg caaggcatta cccgcttcga tgccgaggcg
    10681 ctttaacgcc acggcaatat tcagtgcgca gccgccaacg ttaacgctct gctgtttcag
    10741 ttcgatatcg cacccacgcc aggggagggc ataagcgtcg gcgatcacgt caatgaccgc
    10801 cgcgccgacg accatcaccg gctgacgcgt ggttaattca ggcagcagtg tgtgtaatcg
    10861 agcgccgctc ataccgcctc ccgttgttga cgatatttcg ccagcgctgt ggcatagcgg
    10921 ttgaaatcaa gctgatttac cgcatccagc tccgccttta acgcaggatc gacagcgtta
    10981 atgccatgca acgcgccgca aatcgccgtc gccatagcac ctatggtgtc tgtgtcgccg
    11041 ccaagattgg cgcacaggac ggcgcagcgg tttgggtcgg tttgtgccag ttcaactagc
    11101 gcaatggcgc acggaacgga ctcaatagtg ctggtgcctg cgccaacgac ctggtaaagc
    11161 tgttcgcggg cggattcggt gccgtcggca ttacgcacaa ttttcagcgc aatctccaga
    11221 cgtgccgcca gtgaggcgct gaacgtggtg gtgcgttttt gctgtgcatg tcgcgcaatt
    11281 gaaggcaatg aatcaacaat cgctgaccag cgttctccgt caatggcacg agaaatcgcc
    11341 catgcgatga ctaccgcgcc tgcaaccgcc agatcggatt tatgggtcgg gctggacgcc
    11401 agcgccacat catcaataaa ggaatcaaca tcacgcgctg gaagcaaaca acctaatggc
    11461 gagacgcgca tcgctgcgcc gttggttacg ccgttgtttt ccagttcagc aacgggttta
    11521 ccgtcgcgaa tggcgttaag cgcaatcttc gaggtcggac ctagtacgtt tttgttaaag
    11581 gcgtcgaaac gcaacgccca gtcgagaata ttacgcccaa tcagattcgg atcgatcttg
    11641 ccttcacatt ccagtaacgc atccgccaga cacaggccat cgaggtatcg tcggtgaatt
    11701 cggcgcggtt aaaataacag gccgcgttat tctcctttgg tccaggcaga aaacggtcaa
    11761 tccagccaaa gtgtgcttta acgcggctgc gtggccaaag ctcggagggc atccccatcg
    11821 catcccctaa cgcctgccca taaagagcac cgagaatacg ttctgttttc atttaacttc
    11881 cccttgaatc ggaatttacg ggagcatagt aatgacagat attgcgcagt tgcttggcaa
    11941 agacgccgac aaccttttac agcaccgttg tatgactatt ccttctgacc agctttatct
    12001 ccccggacat gactacgtag accgcgtgat gattgacaat aatcgcccgc cagcggtgtt
    12061 acgtaatatg cagacgttgt acaacaccgg gcgtctggct ggcacaggat atctttctat
    12121 tctgccggtt gaccagggcg ttgagcactc tgccggagct tcatttgctg ctaacccgct
    12181 ctactttgac ccgaaaaaca ttgttgaact ggcgatcgaa gcgggctgta actgtgtggc
    12241 atcaacttac ggcgtgttgg tgtcggtatc gcggcgctat gcgcatcgca ttccatttct
    12301 cgtcaaactt aatcacaacg agacgctaag ttacccgaat acctacgatc aaacgctgta
    12361 tgccagcgtg gagcaggcct tcaacatggg cgcggtggcg gttggtgcga ctatctattt
    12421 tggctcggaa gagtcacgtc gccagattga agaaatttct gcggcctttg aacgcgcgca
    12481 cgagctgggc atggtgacag tgctgtgggc ctatttgcga aactccgcct ttaagaaaga
    12541 tggcgttgat taccatgttt ccgccgacct gactggtcag gcgaaccatc tggcggcaac
    12601 cataggtgca gatatcgtca aacaaaaaat ggcggaaaat aacggcggct ataaagcgat
    12661 taattacggt tataccgacg atcgcgtgta cagcaagtta accagcgaaa acccgattga
    12721 tctggtgcgt tatcagttag ctaactgcta tatgggccgg gctgggttga taaactccgg
    12781 cggtgctgca ggcggtgaaa ctgacctcag cgatgcagtg cgtactgcgg ttatcaacaa
    12841 acgcgcaggc ggaatggggc tgattcttgg acgtaaggcg ttcaagaaat cgatggctga
    12901 cggcgtgaaa ctgattaatg ccgtgcagga cgtttatctc gatagcaaaa ttactattgc
    12961 ctgatacgcc tcatacgaac agcaccaccc cgtaggacgg ataagacgtt tacactgcat
    13021 ccggcaacct atgcctgatg cgacgcggaa gcgtcttatc atgcctacat agcactgcca
    13081 cgtatgttta caccgcatcc ggcataaaaa cacgcgcact ttgctacggc ttccctatcg
    13141 ggaggccgtt tttttgcctt tcactcctcg aacaattttc atattgttgt ttttgcgatc
    13201 attatctcga catttaaaag caaataattt cattatattt tgaaatcgaa agcaaacgac
    13261 aagatatgaa aatgtacgtg gtatcgacaa agcagatgct gaacaacgca cagcgcggcg
    13321 gttatgcggt tccggcattc aatattcaca atctcgaaac gatgcaagtg gtggtggaaa
    13381 ccgctgccaa cctgcatgcg ccggtcatca tcgccggaac gcctggcaca tttactcatg
    13441 ctggtacaga aaatctgttg gcgctggtca gcgcgatggc gaagcaatat caccatccac
    13501 tggcaattca tctcgaccat cacacgaaat ttgacgatat cgctcagaag gttcgttctg
    13561 gcgtgcgctc agtcatgatt gacgcctcgc atttgccttt tgcgcaaaat atttcacggg
    13621 tcaaagaggt ggtggatttt tgccatcgct ttgatgtcag cgtcgaggcg gagctggggc
    13681 aacttggcgg ccaggaagat gatgtgcaag tcaatgaagt cgatgcgttg tacaccaacc
    13741 ccgcacaggc gcgtgaattt gccgaggcaa ccgggattga ttccctggcg gtcgccatcg
    13801 gcacggctca cggtatgtat gccagcgcac cggtgcttga tttttctcga ctggagaaca
    13861 ttcgccagtg ggtgaactta ccgctggtgc tgcatggcgc gtcaggatta tcgactaagg
    13921 atattcagca aaccatcaaa ctggggatat gcaaaatcaa cgttgcaacg gagctgaaaa
    13981 atgccttctt gcagtcgtta aaaaattacc tgaccgagca ccccgaagcg accgatcccc
    14041 gggattattt gcagtcggct aaatccgcaa tgcgcgatgt ggtgagcaaa gtgattgccg
    14101 attgtggctg cgagggcagg gcataacgca cctgccattt aacaaggaaa aaacatgaaa
    14161 acgttaattg cccggcataa a
//
LOCUS       DAJTRC010000093        13706 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.093, whole genome shotgun sequence.
ACCESSION   DAJTRC010000093 DAJTRC010000000
VERSION     DAJTRC010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13706)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13706)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13706
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.093"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            405..1196
                     /locus_tag="OO357_003119"
     CDS             405..1196
                     /locus_tag="OO357_003119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887974.1"
                     /translation="MTTRITFSMTSDETLRIVDEYCHTHKLSRSKVINALLSATAPVL
                     NDINCYYQLAGELQSRLLNGVYQRDLPRKRNVVSAEKYCLEIWENKLFTRRILEFDSS
                     NGVLYALKHKRHYRRDKMIGRVESRCIKDICEYQMQLSGEKTKYACFIYIERTIYNHD
                     NPSDKIPVKAAVGNAVILLAKDVIYNEYFFDLRQSFFVSVTDLMVSGAKGIPETQTYP
                     DVYCWIPLFSINSGVLITPVYKIDPLKPVTVKKPDKITVVCNYRE"
     gene            1582..1788
                     /locus_tag="OO357_003120"
     CDS             1582..1788
                     /locus_tag="OO357_003120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5887975.1"
                     /translation="MNTPVSLMDDQMVDMSFITQLTGLTDKWFYKLIKHGAFPPPIKL
                     GRSSRWLKSEVEAWLQARIAQSRP"
     gene            1874..2446
                     /locus_tag="OO357_003121"
     CDS             1874..2446
                     /locus_tag="OO357_003121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887976.1"
                     /translation="MQQLENRCDLLLIQHQKWMTSVTRLIVAHGMGSPHLHGYHRLTL
                     AHFFLPEKGSVISVAPQGLYQVVNPGTPPFIPAIQEGLMTSIQTHEIMLLTHFNLGGV
                     LLSELHRLGENRLANRLNSLLRRFDDRDLYHTLIWLCWYDLMCAHSMQPWTEELKHKS
                     HAELENWAVARKREKRELELMIDEYLLYAC"
     gene            complement(2448..2732)
                     /locus_tag="OO357_003122"
     CDS             complement(2448..2732)
                     /locus_tag="OO357_003122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000261147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887977.1"
                     /translation="MVVLNTPGKYQYDLRNHLLVNLFPIALDAEITTSNVNKHQLHRS
                     NIPAALSGNSGITDYVPFSAERNAAMVIWRTAIDSRPAVCNDEEGTPGTL"
     gene            2703..2846
                     /locus_tag="OO357_003123"
                     /pseudo
     CDS             2703..2846
                     /locus_tag="OO357_003123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            4015..5034
                     /locus_tag="OO357_003124"
     CDS             4015..5034
                     /locus_tag="OO357_003124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5887978.1"
                     /translation="MNNEQMLIKCRDPRKCRFYAEYLNHKYPNEFPHFAPDNPQNCIR
                     NVNLFLKEKYCNVSPPPFGTFGTTEEEALNNEINELEIIAGQAILPEESFLWLKTDEL
                     ATFFTWTTIYLSKEDHTKFPKINHNSSGIKNGIEVRKIRTVTDETLNTYKKSNLPDNP
                     SSHNERIKVIISYFDSCHSLINKNAKSIYLEELRQAWLKKKSACQKFKWLLPDDDEMC
                     QWFWTRLKQKQDEKHPVHNPVTRWFIPSSTKEYYLASLVAFALWKEAPDTIELFRTRI
                     NHAWHQKKQKDKYRSDGKKAINIHIRSNVKEMLDELRTVYGLRTGEFFEMLITEKYLE
                     HKRRK"
     gene            5144..6016
                     /locus_tag="OO357_003125"
     CDS             5144..6016
                     /locus_tag="OO357_003125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase family protein"
                     /protein_id="HCR5887979.1"
                     /translation="MNPSDAIEAIEKPLSSLPYSLSRHILEHLRKLTSHEPVIGIMGK
                     SGAGKSSLCNALFQGEVTPVSDVHAGTREVQRFRLSGHGHSMVITDLPGVGESRDRDA
                     EYEALYRDILPELDLVLWLIKADDRALSVDEYFWRHILQCGHQQVLFVVTQADKTEPC
                     HEWDMAGIQPSPAQEQNIREKTEAVFRLFRPVHPVVAVSARTGWELDTLVSALMTALP
                     DHAASPLMTRLQDELRTESVRSQAREQFTGAVDRIFDTAESVCVASVVRTALRAVRDT
                     VVSVARAVWNWIFF"
     gene            6350..9472
                     /gene="ag43"
                     /locus_tag="OO357_003126"
     CDS             6350..9472
                     /gene="ag43"
                     /locus_tag="OO357_003126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter adhesin Ag43"
                     /protein_id="HCR5887980.1"
                     /translation="MKRHLNTCYRLVWNHITGAFVVASELARAQGKRGGVAVALSLAA
                     VTSLPVLAADIVVHPGETVNGGTLVNHDNQFVSGTADGVTVSTGLELGPDSDENTGGQ
                     WIKAGGTGRNTTVTANGRQIVQAGGTASDTVIRDGGGQSLNGLAVNTTLDNRGEQWVH
                     GGGKAAGTIINQDGYQTIKHGGLATGTIVNTGAEGGPESENVSSGQMVGGTAESTTIN
                     KNGRQVIWSSGMARDTLIYAGGDQTVHGEAHNTRLEGGNQYVHNGGTATETLINRDGW
                     QVIKEGGTAAHTTINQKGKLQVNAGGKASDVTQNTGGALVTSTAATVTGTNRLGAFSV
                     VEGKADNVVLENGGRLDVLTGHTATNTRVDDGGTLDVRNGGTATTVSMGNGGVLLADS
                     GAAVSGTRSDGKAFSIGGGQADALMLEKGSSFTLNAGDTATDTTVNGGLFTARGGTLA
                     GTTTLNNGAILTLSGKTVNNDTLTIREGDALLQGGALTGNGSVEKSGSGTLTVSNTTL
                     TQKAVNLNEGTLTLNDSTVTTDVIAQRGTALKLTGSTVLNGAIDPTNVTLASGATWNI
                     PDNATVQSVVDDLSHAGQIHFTSTRTGKFVPATLKVKNLNGQNGTISLRVRPDMAQNN
                     ADRLVIDGGRATGKTILNLVNAGNSASGLATSGKGIQVVEAINGATTEEGAFIQGNKL
                     QAGAFNYSLNRDSDESWYLRSENAYRAEVPLYASMLTQAMDYDRILAGSRSHQTGVSG
                     ENNSVRLSIQGGHLGHDNNGGIARGATPESSGSYGFVRLEGDLLRTEVAGMSVTAGVY
                     GAAGHSSVDVKDDDGSRAGTVRDDAGSLGGYLNLIHNASGLWADIVAQGTRHSMKASS
                     DNNDFRVRGWGWLGSLETGLPFSITDNLMLEPQLQYTWQGLSLDDGQDNASYVKFGHG
                     SAQHVRAGFRLGSHHDMNFGKGTSSRDTLRGSAKHSVRELPVNWWVQPSVIRTFSSRG
                     DMSMGTAAAGSNMTFSPSQNGTSLDLQAGLEARVRENITLGVQASYAHSINGSSAEGY
                     NSQATLNVTF"
     gene            9593..12109
                     /locus_tag="OO357_003127"
     CDS             9593..12109
                     /locus_tag="OO357_003127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887981.1"
                     /translation="MLQIVGALILLIAGFAILRLLFRALTSTASALAGFILLCLFGPA
                     LLAGYITERITRLFHIRWLAGVFLTIAGMIISFMWGLDGKHIALEAHTFDSVKFILTT
                     ALAAGLLALPVQIRTIQQNGLTPEDISKEINGYYCCFYTAFFLMACSAYAPLIALQFD
                     ISPSLMWWGGLLYWLAALVTLLWAASQIQALKRLTSAISQTLEEQPVLNSKSWLSSLQ
                     NDYSLPETLTERIWLTLISQRISRGELREFELADGNWLLNNAWYERNMAGFNEQLKEN
                     LSFTPDELKTLFRNRLNLSPEANDDFLDRCLDGGDWYPFSEGRRFVSFHHVDELRICA
                     SCGLTEVHHAPENHKPAPEWYCSSLCHETETLCQDIYERSYTGFISDATANGLILMKL
                     PETWSTNEKMFASGGQGHGFAAERGNHIVDRVRLKNARILGDNNARNGADRLVSGTEI
                     QTKYCSTAARSVGAAFDGQNGQYRYMGNHGPMQLEVPRDQYAGAVETMKNKIREGKVP
                     GVTDPAEASRLIRRGHLTYTQARNITRFGTIESVTYDIAEGSVVSLAAGGISFALTAS
                     VFWLSTGDRDAALQTAAVQAGKTFTRTLAVYVTTQQLHRLTVVQGMLKHIDFSTASPT
                     VRQALQKGTGAGNISALNKVMKGTLVTSLALVAVTTSPDMIKMLRGRISGAQFIRNLA
                     VASSGVAGGAVGSVAGGILFSPLGPFGALTGRVVGGVLGGMIASAVSGKIAGALVEED
                     RVKILAMIQEQVTWLAGSFLLTGHEIENLNANLARVIDQNALEIIFAAGIQQRAATNM
                     LIKPLVVSIIRQRPVMEYDASHLGKMVNRLEEAFPPELPA"
     gene            12185..12640
                     /locus_tag="OO357_003128"
     CDS             12185..12640
                     /locus_tag="OO357_003128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5887982.1"
                     /translation="MIHLFKTCMITAFILGLTWSAPLRAQDQRYISIRNTDTIWLPGN
                     ICAYQFRLDNGGNGEGFGPLTITLQLKDKYGQTLVTRKMETEAFGDSNATRTTDAFLE
                     TECVENVATTEIIKATEESNGHRVSLPLSVFDPQDYHPLLITVSGKNVN"
     gene            complement(12759..12970)
                     /locus_tag="OO357_003129"
                     /pseudo
     CDS             complement(12759..12970)
                     /locus_tag="OO357_003129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001386164.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            13051..>13706
                     /locus_tag="OO357_003130"
     CDS             13051..>13706
                     /locus_tag="OO357_003130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5887983.1"
                     /translation="MQLVSRFGYANQIRRDRPLTHEELMHHVPGIFGEEKHTSRSQNY
                     TYIPTITVLESLQREGFQPFFACQTRVRDPGRRGYTKHMLRLRRAGEINGEHVPEIIL
                     LNSHDGTSSYQMLPGYFRFVCQNGCVCGQSLGEVRVPHRGNVVEKVIEGAYEVVGVFD
                     RIEEKRDAMQSLVLPPPARQALAQAALTYRYGDEHQPVTTADILTPRRREDYGKDLWS
                     T"
BASE COUNT         3668 a         3402 c         3388 g         3248 t
ORIGIN      
        1 agtatatctt tatgagagat ccgtctacag gattgggtta taaggtgtat ttacaaggta
       61 taatcacagg atcatttaca tgaccatact tctgtaaata atactaatta accttaatta
      121 tttttttatg gtacatgaac tacctgtagc tgtaacgata aacaaatcac ccaaaatgaa
      181 aaatactggt tatcacagtt cagagaactc gtatgagtgt ccccctgagc aatgcttcat
      241 tttctgtgat tctgtgtgat agagttatac tgagtacagt ggcagtaaca ctgctttcca
      301 tcacttgagg ataaaattca ttaaaaaatt aattgcatga attaacaggt ttttttatga
      361 aatgttatgc atctcaacca atatgattaa cagaaaactt catcatgaca acacgtataa
      421 cgttcagtat gaccagtgat gagacgctgc gaattgtgga tgagtactgt catacccaca
      481 aactttcgcg ctctaaggta atcaacgcat tactcagcgc cacagcacct gtccttaatg
      541 atattaactg ttattatcaa cttgcaggcg aactgcagtc acgcttatta aatggagtgt
      601 atcaacggga tcttcctcgc aaacgcaatg tcgtcagtgc agagaagtat tgtctggaaa
      661 tctgggaaaa caaacttttc accagaagaa tcctggagtt tgacagttca aatggtgttc
      721 tgtacgcgct gaaacataaa agacactatc gccgggataa gatgattgga agggttgaaa
      781 gtcggtgtat caaagacatt tgcgaatacc agatgcagct atccggggag aagacgaaat
      841 atgcctgttt tatttatatc gaaagaacaa tatacaatca cgataatcca tctgataaaa
      901 tacctgtaaa agcggctgtt ggtaatgcag tcatcctgct ggcaaaggat gtgatatata
      961 acgagtactt ttttgatttg cggcagtcat tttttgtgag cgttacagat ctgatggtct
     1021 ctggcgcaaa aggtattccg gagacacaga catatccgga cgtttattgc tggatccctc
     1081 ttttttccat aaattccggc gtgctcatta cccctgtata taaaattgac cctctgaaac
     1141 ccgtaacggt aaaaaaaccg gataagatca ctgttgtttg taattacaga gaataatttc
     1201 agccttctac cttcaccgcg acagtgttta cctctgcttt atcttccgtt cagtaaatac
     1261 aaaccaccgc tctggcaggt acagggcgtg gtttatctcc gatactcccc tgtgcagtaa
     1321 taatcatcac tgaaaatact gatcccgtca cggcaaaacg ccatcccata ttccggcaac
     1381 acaaaatcat cttccgttta taccggattc actgacaaaa cagacaacct gtattgcctg
     1441 tacacaacag tttctctcct ggcaggaata ttcccctact cattttttac gtttctcccc
     1501 ggttgttatg ccggtgaagg tggtgcgtcg ttttcatcac caacgccggt tgagattaac
     1561 aacatccgga ggaacattct catgaatacc ccagtttcac tgatggatga ccagatggtc
     1621 gacatgtcgt ttatcactca gctgaccggc ctgaccgata agtggtttta taaactcatc
     1681 aagcatggag cctttccgcc ccccatcaaa ctgggccgta gctcccgctg gctgaaaagc
     1741 gaagtggaag cctggctgca ggcgcgtatt gcacagtccc gtccgtaatt cctgaccgtt
     1801 atccgttcca ccgcagcaaa ggcatgatcc ggcccccgga ggcattttgc tgcgcgctaa
     1861 aggagagaat attatgcaac aacttgaaaa caggtgtgac ctgctgctca tccagcatca
     1921 gaaatggatg acaagcgtga cgaggcttat cgtcgctcat ggaatggggt ctccgcatct
     1981 tcatggttat caccgtctga ccctggcaca cttttttctg ccggaaaaag gctccgtcat
     2041 ttcggtggcc ccccagggac tctatcaggt cgtcaacccg ggaacaccac cgttcattcc
     2101 tgccattcag gaagggttaa tgaccagcat tcagacccat gaaatcatgc tgcttacaca
     2161 ttttaatctg ggcggcgttt tactcagtga gctacatcgt ctgggtgaaa accggctggc
     2221 aaacaggctg aacagcctgc tccggcgttt cgacgatcgt gatctctacc acacgctgat
     2281 atggctgtgc tggtatgatc tgatgtgtgc ccactccatg cagccctgga ctgaagagtt
     2341 aaagcataaa tcacatgcag agcttgaaaa ctgggctgtg gcgcgcaaac gggaaaagcg
     2401 cgagctggaa ctgatgattg acgagtacct gttatacgcc tgctgattca taacgttccc
     2461 ggggttcctt cctcatcgtt acacacagcc ggacggctat ctatggctgt ccgccaaatg
     2521 accatcgccg catttcgttc cgctgaaaac ggtacgtagt cagtgatacc ggaatttccg
     2581 gagagggccg caggtatatt gcttctgtgc aattgatgct tatttacatt ggatgtagtt
     2641 atttctgcat ccaatgctat cggaaagaga ttaaccagca aatggttcct caaatcgtat
     2701 tgatatttac ccggcgtatt gagcaccacc atctgacact ccgttcccgc atcaaacgac
     2761 tggaccgaaa aacgatttac ttttcacgtg ctgtggaaat tcacgaaaaa attattgggt
     2821 cattcatcga aaaacatatg ttctgatgac tggagtcatc ctccagaaat atacgtcaac
     2881 tgaacaatat cgatttttta tgggattcta tacacttttc catatccctc tgaaaatcag
     2941 tcatcagctc accaacacct cttctgaaac ttgtattaaa cttattgctt aaatagatga
     3001 tataacatct cttccattgc actgaatcgg cgaaaactgc cgtttcaata atctcaacaa
     3061 tattatttat cggctaactt tatcagtaaa caatggaatg tcacactgat gaaatgaaca
     3121 ttcagagtaa gatgataaaa ttcatataaa cgccattgct gaatattatt tatttcatac
     3181 agataaacga taaaatacaa aagggtatat ttctcaatgt aacagtcaat tattcatctc
     3241 cactggcgag cacggtcagc acacaaacaa agttagtcct cctgcttaat cattgcgtat
     3301 cagaaactaa aaaagaaaaa attctgcaga caacttaaca atcttcatca catttcctga
     3361 agacagacat atccacaaac ggtagtcagc gttgaattaa caagtcttac agagctatac
     3421 gtagcttata aagccactca atgttcatca tcacccgact acttatctgg aataccacca
     3481 gtataaactc atcaataaaa acacctgttg ttaaaataag aatactgtca gcagtaaatt
     3541 attaaaacaa catctgtcca cactatctgt tcttttaacg ccaacggcag cgaaaatagt
     3601 gcatcacagc cttctttttt ctttcgccac cactcagaca aacacaacac tcacacccac
     3661 accacaaaac agaatcaaaa cataaaatta acaataacag tcaaaaggaa aacacagaat
     3721 attgaggtgg actgtcacca ggacagcgca tacgacgcat gtgatcagca catcccgttc
     3781 ttcgctggca tccccaaaat gcagaacaaa caatcataac attatgaata taaaagaaaa
     3841 tatgattttt caccatgaaa aaaacatcaa tgtatctctg tataaaaata atgataacat
     3901 gacataaaca tttaaaaaca taaataaaca gcaacaaaaa catttgataa tcacttagtg
     3961 attaaataca attaagacga ttttgttata agagaggcac catggagtga gataatgaat
     4021 aacgaacaga tgcttatcaa atgcagagat ccccgtaaat gtcggtttta tgccgaatat
     4081 ctgaatcaca aataccctaa tgaattccct cactttgcgc ctgacaaccc acaaaattgt
     4141 atcaggaatg ttaatttatt tttaaaggaa aagtattgta atgtttcacc tccccccttt
     4201 ggtacttttg gtactactga agaagaagca ctcaataacg aaattaatga gcttgaaatt
     4261 attgccgggc aggcaatact gcctgaagag agttttctct ggttgaaaac tgacgaactt
     4321 gcgactttct ttacctggac tactatttat ctgtcgaagg aagaccatac aaaattcccg
     4381 aaaataaacc ataattcatc aggaataaaa aacggaattg aagtcaggaa aatacgaaca
     4441 gttaccgatg aaacgttaaa cacttacaaa aaaagtaacc ttccggataa tccatcatcc
     4501 cataacgaac gaataaaagt aatcatttca tattttgact cctgccattc tttaattaat
     4561 aaaaatgcaa aaagcatata cctcgaggaa ctcaggcagg cgtggcttaa aaagaaaagc
     4621 gcttgtcaaa agtttaagtg gctccttcct gacgatgatg aaatgtgtca gtggttctgg
     4681 acaagactga aacaaaaaca ggatgaaaaa catccagtac ataacccagt gacccggtgg
     4741 ttcataccat cttcaacgaa agaatattat cttgcctctc ttgtcgcctt tgctttatgg
     4801 aaagaagcgc ctgataccat tgaactgttc agaacacgaa taaatcatgc ctggcatcag
     4861 aaaaaacaaa aggacaaata tcggagtgat ggtaaaaagg ccattaatat acatataaga
     4921 agcaatgtga aagaaatgct tgatgagctc agaactgttt acggtctgag aacaggagag
     4981 ttttttgaaa tgctgattac agagaaatat cttgaacaca agcgaaggaa gtaattcatt
     5041 acctgttaca ttctaaataa tctcagacag atattatctc cgtgaatccc ccgccacctt
     5101 tccggtgcgc ggggttttgt cttttttcac cgggaataca tgtatgaatc cgtctgatgc
     5161 cattgaggca attgaaaaac cgctctcctc cctgccttac tcactttccc gtcacatcct
     5221 ggaacatctg cgcaaactca ccagtcacga acccgtgatt ggcattatgg gtaaaagcgg
     5281 ggccggtaaa tcttcactct gtaatgcact gtttcagggg gaggtcacac cggtcagtga
     5341 tgttcacgcc ggcacccggg aagtgcagcg cttccgtctg agtggtcacg gtcacagcat
     5401 ggttatcact gacctgcccg gggtgggcga gagccgggac agggatgcag agtatgaagc
     5461 cctgtaccgt gacattctgc ctgaactgga cctggtgctg tggctgatta aagccgatga
     5521 ccgggccctg tctgtggatg agtatttctg gcgacacatc ctgcagtgcg gacatcagca
     5581 ggtgctgttt gtggtgacgc aggctgacaa aacggaaccc tgccatgaat gggatatggc
     5641 aggtattcag ccttctcccg cacaggaaca gaacattcgt gagaaaacgg aggcggtatt
     5701 ccgtctgttc cggcccgtac atccggttgt tgccgtatcg gcccgcaccg gctgggaact
     5761 ggatacgctg gtcagtgcgc tcatgacagc gcttcccgac catgccgcca gccccctgat
     5821 gacccgactg caggacgagc tgcgcacgga gtctgtccgc tctcaggccc gtgaacagtt
     5881 taccggtgcg gtggaccgga tatttgacac agcggagagc gtctgtgttg cctctgttgt
     5941 ccgtacggcc ctgcgcgctg ttcgtgacac cgtggtctct gttgcccgcg cggtatggaa
     6001 ctggatcttc ttctgaacct gttgtggatg atgtcctccc tgcctctgag tctgctcaca
     6061 aaagcgctgt tttcgttact gtctctcttg tccgtgcaat agactgataa tagaataaaa
     6121 cgatcgatac ctattttacc gatcgttaat atcgatcgtt atgctaataa taactcctgt
     6181 tagcaacgtg cgcagataca cacagacatg agattcaggg aacaacagag ccacacgtca
     6241 gaaacttccg tcagctggac ctccggcact gtaacccttt acctgccggt atccacatct
     6301 gtggataccg gcttttttat tcaccctcac tctgattaag gaaatgctga tgaaacgaca
     6361 tctgaatacc tgctacaggc tggtatggaa tcacattacg ggcgctttcg tggttgcctc
     6421 cgaactggcc cgcgcacagg gtaaacgtgg cggtgtggcg gttgcactgt ctcttgccgc
     6481 ggtcacgtca ctcccggtgc tggctgctga catcgttgtg cacccgggtg aaacagtgaa
     6541 tggcggaaca ctggtaaacc atgacaacca gtttgtatcc ggaacagctg atggcgtgac
     6601 tgtcagtacc gggcttgagc tggggccgga cagtgacgaa aacaccggcg ggcaatggat
     6661 aaaagcgggt ggcacaggca gaaacaccac tgtcaccgca aatggtcgtc agattgtgca
     6721 ggcaggagga actgccagtg atacggttat tcgtgatggc ggagggcaga gccttaacgg
     6781 actggcggtg aacaccacgc tggataacag aggtgagcag tgggtacacg ggggagggaa
     6841 agctgccggt acaattatta accaggatgg ttaccagacc ataaaacatg gcggactggc
     6901 aaccggaacc atcgtcaaca ccggtgcaga aggtggtccg gagtctgaaa atgtgtccag
     6961 cggtcagatg gtcggaggga cggctgaatc caccaccatc aataaaaatg gccggcaggt
     7021 tatctggtct tcggggatgg cacgggacac cctcatttac gccggtggtg accagacggt
     7081 acacggagag gcacataaca cccgactgga ggggggtaac cagtatgtac acaacggtgg
     7141 cacggcaaca gagacgctga taaaccgtga tggctggcag gtgattaagg aaggaggaac
     7201 tgccgcgcat accaccatca accagaaagg aaagctgcag gtgaatgccg gcggtaaagc
     7261 gtctgatgtc acccagaaca cgggcggtgc actggttacc agtacagctg caaccgtcac
     7321 cggcacaaac cgcctgggag cattctctgt tgtggagggt aaagctgata atgtcgtact
     7381 ggaaaatggc ggacgcctgg atgtgctgac cggacacaca gccactaata cccgcgtgga
     7441 tgatggcgga acgctggatg tccgcaacgg tggcaccgcc accaccgtat ccatgggaaa
     7501 tggcggtgta ctgctggccg attccggtgc cgctgtcagt ggtacccgga gcgacggaaa
     7561 ggcattcagt atcggaggcg gtcaggcgga tgccctgatg ctggaaaaag gcagttcatt
     7621 cacgctgaac gccggtgata cggccacgga taccacggta aatggcggac tgttcaccgc
     7681 caggggcggc acactggcgg gcaccaccac gctgaataac ggcgccatac ttaccctttc
     7741 cgggaagacg gtgaacaacg ataccctgac catccgtgaa ggcgatgcac tcctgcaggg
     7801 aggcgctctc accggtaacg gcagcgtgga aaaatcagga agtggcacac tcactgtcag
     7861 caacaccaca ctcacccaga aagccgtcaa cctgaatgaa ggcacgctga cgctgaacga
     7921 cagtaccgtc accacggatg tcattgctca gcgcggtaca gccctgaagc tgaccggcag
     7981 cactgtgctg aacggtgcca ttgaccccac gaatgtcact ctcgcctctg gtgccacctg
     8041 gaatattccc gataacgcca cggtgcagtc ggtggtggat gacctcagcc atgccggaca
     8101 gattcatttc acctccaccc gcacagggaa gttcgtaccg gcaaccctga aagtgaaaaa
     8161 cctgaacgga cagaatggca ccatcagcct gcgtgtacgc ccggatatgg cacagaacaa
     8221 tgctgacaga ctggtcattg acggtggcag ggcaaccgga aaaaccatcc tgaacctggt
     8281 gaacgccggc aacagtgcgt cggggctggc gaccagcggt aagggtattc aggtggtgga
     8341 agccattaac ggtgccacca cggaggaagg ggcctttatc caggggaata agctgcaggc
     8401 cggtgccttt aactactccc tcaaccggga cagtgatgag agctggtatc tgcgcagtga
     8461 aaatgcttat cgtgcagaag tccccctgta tgcctccatg ctgacacagg caatggacta
     8521 tgaccggatt ctggcaggct cccgcagcca tcagaccggt gtaagcggtg aaaataacag
     8581 cgtccgtctc agcattcagg gcggtcatct cggtcacgat aacaacggcg gtattgcccg
     8641 tggagccacg ccggaaagca gcggcagcta tggcttcgtc cgtctggagg gtgacctgct
     8701 cagaacagag gttgccggta tgtctgtgac cgcgggggta tatggtgctg ctggccattc
     8761 ttccgttgat gttaaggatg atgacggctc ccgtgccggc acggtccggg atgatgccgg
     8821 cagcctgggc ggatacctga atctgataca caacgcctcc ggcctgtggg ctgacattgt
     8881 ggcccaggga acccgccaca gcatgaaagc ctcatcggac aataacgact tccgcgtccg
     8941 gggctggggc tggctgggtt cgctggaaac cggtctgccc ttcagtatca ctgacaatct
     9001 gatgctggag ccgcaactgc agtacacctg gcagggactc tccctggatg acggccagga
     9061 taacgccagt tatgtgaagt tcgggcatgg cagtgcacaa catgtgcgtg ccggcttccg
     9121 tctgggcagc caccacgata tgaactttgg taaaggcacc tcatcccgtg acaccctgcg
     9181 cggcagtgcc aaacacagtg tgcgtgaact gccggtgaac tggtgggtac agccttctgt
     9241 tatccgcacc ttcagctccc ggggggacat gagcatgggt acagccgcag ccggcagtaa
     9301 catgacgttc tcaccgtcac agaatggtac gtcactggac ctgcaggccg gactggaagc
     9361 ccgtgtccgg gaaaatatca ccctgggcgt tcaggccagt tatgcccaca gcatcaacgg
     9421 cagcagcgct gaaggttata acagtcaggc cacactgaat gtaaccttct gataattctg
     9481 cattgcctct ctgtggtccc ggtcatgatg accgggaccc ggacggggga aaacgtttca
     9541 gtgccacatt cactggcatt cacaataaca tgatattcat cacggagtga ctatgttaca
     9601 gatagtcggc gcgctgattc tgctgatcgc aggatttgcc attcttcgcc ttttgttcag
     9661 agcattaacc agcacagcgt ctgcgctggc agggttcata ttgctgtgtc tgttcggtcc
     9721 ggctttactg gctggctata tcactgaacg cataacccgg ttattccata ttcgctggct
     9781 ggcaggcgta tttctgacga ttgccggaat gatcatcagc ttcatgtggg gacttgatgg
     9841 taaacatatc gcactggagg ctcatacttt tgactctgta aaatttattc tgaccaccgc
     9901 tctcgccgct ggtctgctgg ctcttcccgt gcagataaga accattcagc agaacgggct
     9961 cacacctgaa gatatcagca aggaaattaa cgggtattac tgctgttttt atactgcttt
    10021 tttccttatg gcgtgttctg catacgcacc attgatcgca ttgcagttcg atatttcacc
    10081 ctcactgatg tggtggggcg ggttgttgta ctggctggct gcattagtga cgctgctatg
    10141 ggcggccagc cagatccagg cgctgaaaag actgaccagt gccatcagcc agacactgga
    10201 agaacaaccg gtgctcaaca gtaaatcgtg gctgagcagt ttgcaaaacg attacagcct
    10261 tcctgaaacg ctgacggagc gcatctggct gacactcatt tcacaacgga tttcccgggg
    10321 agaactgagg gaatttgaac tggcagacgg aaactggtta ctgaacaatg cctggtatga
    10381 aagaaacatg gcagggttta acgaacagtt gaaagagaac ctgtcattca cacctgatga
    10441 actgaaaacg ctcttccgga accgcctgaa tttatcaccg gaagcgaatg acgattttct
    10501 cgatcgttgc ctggacggcg gtgactggta ccccttttca gaaggccgcc gttttgtatc
    10561 attccaccac gtggatgagc ttcgtatctg tgcctcctgc gggctgacag aagtacatca
    10621 tgccccggaa aatcataagc cggctccgga atggtactgc tcctctcttt gtcacgaaac
    10681 agaaacactg tgtcaggaca tttatgaacg ttcttacacc ggttttattt ccgatgcaac
    10741 ggcgaatggt ctgattctca tgaaactgcc ggaaacctgg agtacaaatg agaaaatgtt
    10801 tgcttccgga gggcagggac atgggtttgc cgctgaacgg ggaaaccata ttgtcgacag
    10861 agtccgtctg aaaaacgcac ggatcctcgg tgataataat gccagaaatg gagcagacag
    10921 actggtcagc ggaacagaaa tccagacgaa atattgttca actgcagccc gtagcgtcgg
    10981 tgcggcattc gacggacaaa acggacagta tcgttacatg ggaaatcatg gtcccatgca
    11041 actggaagtc ccccgtgatc agtatgccgg cgctgtggaa accatgaaga ataagatccg
    11101 cgaaggtaaa gtacccggtg taaccgatcc cgcagaagcg tcccggctga ttcgtcgggg
    11161 acatctgact tatacccagg cccgtaatat cacccggttc gggaccatcg aatcggtcac
    11221 ttatgatatt gccgaggggt cggttgtcag tctggcggcc ggagggatca gttttgccct
    11281 gacggcatcg gtcttctggc tcagcaccgg cgatcgcgat gctgccctgc agacagctgc
    11341 tgtccaggca ggaaaaacct tcacccgcac actggctgtc tacgtcacaa cccagcaact
    11401 tcaccggctc actgttgttc agggtatgct gaagcatatt gatttttcga cggccagccc
    11461 gactgtccgg caggcgcttc agaaggggac cggtgcagga aatatcagtg ccctgaacaa
    11521 agtgatgaag ggtacgctgg tgacatctct ggcactggta gctgtcacaa ccagccctga
    11581 catgatcaaa atgttgcggg gacggatctc cggtgcgcag ttcatcagga atcttgccgt
    11641 ggcatcttcc ggtgtggcag gtggtgctgt cgggtcagtg gcgggcggga tattgttcag
    11701 tccactggga ccatttggtg cactgacagg gcgtgtggtt ggcggtgttc tggggggaat
    11761 gattgcctcc gctgtatcag gaaaaattgc cggagcgctg gttgaagaag atcgcgtcaa
    11821 aattctggca atgattcagg agcaggtgac atggcttgcc ggcagtttcc tgctgaccgg
    11881 acatgagatt gaaaatctga acgcgaatct ggcccgtgtt atcgatcaga atgctctgga
    11941 gatcattttc gccgccggta tacaacaacg ggccgcgacc aatatgttaa tcaaaccact
    12001 ggtggtcagt atcatcaggc aacgccccgt catggaatat gatgcatccc atctcggcaa
    12061 gatggttaac cggctggaag aagcattccc cccggagctc ccggcataac gtgtgttctg
    12121 ctccttttag gttttatcac ctgatttgcc agtgtcatcg tcatgactca gagaggtaaa
    12181 taccatgatt cacctgttca aaacctgcat gattaccgcc ttcattctgg ggttaacgtg
    12241 gtctgcccca ctccgggcac aggatcaacg ttacatcagt atacgcaata cagatacgat
    12301 atggctcccg ggaaatattt gtgcttacca gttccggctg gataatggcg gaaacggtga
    12361 aggatttggc cccctcacca tcactctgca actcaaagac aaatatggtc agacgctggt
    12421 gaccagaaaa atggaaacgg aagcttttgg tgacagtaat gccacgcgaa ccacagacgc
    12481 atttctggaa acggagtgcg tggaaaatgt cgccacaacc gaaatcatta aagcaactga
    12541 agaaagtaac ggccatcgtg tcagtctgcc gttatcggtt ttcgatcccc aggactacca
    12601 tccactgctg attaccgttt ccggaaaaaa tgttaactga tccctgaagt cagtttcatg
    12661 cttcagcctc tgccacaacg tatggcagag gctatttatc aggagaaacc agatatgccg
    12721 ggttacacac agagtatgtc ctggctgaag gatctttctc atacgggcag gcggttgctg
    12781 tcataaccgc ttaccgcaat gtgtttatcc aggatgaccc gggaatgcat ttccgtcggg
    12841 ttatccgcaa cgcggaaggt cagcgacgct gacgcagccg gaactctgaa gctgatgccg
    12901 gaaaacagct taatgcgtgg ctcgccagtg gagggcttct cagacaataa acttcattga
    12961 tttcatccat caggccgcgt cttctccggg agacgcggct tttttcattt gttgcaccag
    13021 taaatcttaa ccaccgataa ggagcaaagt atgcaattag tcagtcgttt tggttatgcg
    13081 aaccagatac gccgtgaccg tccgctgaca catgaagagc tgatgcacca tgtgccgggg
    13141 attttcgggg aagagaaaca cacgtcccga agccagaact atacgtacat ccccaccatc
    13201 accgtactgg aaagtctgca gcgggaaggc ttccagccct ttttcgcctg ccagacccgt
    13261 gtgcgcgacc cgggccgccg gggatacaca aaacacatgc tgcgtctgcg gcgggccgga
    13321 gagataaacg gagaacatgt ccctgaaatt attctgctca actcccatga cggtacctcc
    13381 agctaccaga tgctgccggg ttacttcaga ttcgtctgcc agaacgggtg tgtctgtggt
    13441 cagtctctgg gggaagtgcg tgttccgcac cggggaaacg tggtggagaa agttattgaa
    13501 ggggcttacg aagtggtggg cgtgtttgac cggattgagg agaaacgtga tgccatgcag
    13561 tcgctggtcc tgccgccacc ggcacgccag gcgctggcac aggcggcact gacttaccgt
    13621 tatggtgacg aacatcagcc cgtcaccacc gccgacattc tgacgccacg acgccgggag
    13681 gattacggta aggacctgtg gagtac
//
LOCUS       DAJTRC010000094        13618 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.094, whole genome shotgun sequence.
ACCESSION   DAJTRC010000094 DAJTRC010000000
VERSION     DAJTRC010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13618)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13618)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13618
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.094"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO357_003131"
     CDS             complement(99..1778)
                     /gene="bcsG"
                     /locus_tag="OO357_003131"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsG"
                     /protein_id="HCR5887984.1"
                     /translation="MTQFTQNTAMPSSLWQYWRGLSGWNFYFLVKFGLLWAGYLNFHP
                     LLNLVFAAFLLMPIPRYSLHRLRHWIALPIGFALFWHDIWLPGPESIMSQGSQVAGFS
                     TDYLIDLVTRFINWQMIGAIFVLLVAWLFLSQWIRITVFVVAILLWLNVLTLAGPSFS
                     LWPAGQPTTTVTTTGGNAAATVAATGGAPVVGDMPAQTAPPTTANLNAWLNNFYNAEA
                     KRKSTFPSSLPADAQPFELLVINICSLSWSDIEAAGLMSHPLWSHFDIDFKNFNSATS
                     YSGPAAIRLLRASCGQTSHTNLYQPANNDCYLFDNLSKLGFTQHLMMGHNGQFGGFLK
                     EVRENGGMQTELMDQTNLPVILLGFDGSPVYDDTAVLNRWLDVTEKDKNSRSATFYNT
                     LPLHDGNHYPGVSKTADYKARAQKFFDELDAFFTELEKSGRKVMVVVVPEHGGALKGD
                     RMQVSGLRDIPSPSITDVPVGVKFFGMKAPHQGAPIVIDQPSSFLAISDLVVRVLDGK
                     IFTEDNVDWKKLTSGLPQTAPVSENSNAVVIQYQDKPYVRLNGGDWVPYPQ"
     gene            complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO357_003132"
     CDS             complement(1775..1966)
                     /gene="bcsF"
                     /locus_tag="OO357_003132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsF"
                     /protein_id="HCR5887985.1"
                     /translation="MMTISDIIEIIIVCALIFFPLGYLARHSLRRIRDTLRLFFAKPR
                     YVKPAGTLRRTEKARATKK"
     gene            complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO357_003133"
     CDS             complement(1963..3534)
                     /gene="bcsE"
                     /locus_tag="OO357_003133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsE"
                     /protein_id="HCR5887986.1"
                     /translation="MRDIVDPVFSIGISSLWDELRHMPAGGVWWFNVDRHEDAISLAN
                     QTIASQAETAHVAVISMDSDPAKIFQLDDSQGPEKIKLFSMLNHEKGLYYLARDLQCS
                     IDPHNYLFILVCANNAWQNIPAERLRSWLDKMNKWSRLNHCSLLVINPGNNNDKQFSL
                     LLEEYRSLFGLASLRFQGDQHLLDIAFWCNEKGVSARQQLSVQQQNGIWTLVQSEEAE
                     IQPRSDEKRILSNVAVLEGAPPLSEHWQLFNNNEVLFNEARTAQAATVVFSLQQNAQI
                     EPLARSIHTLRRQRGSAMKILVRENTASLRATDERLLLACGANMVIPWNAPLSRCLTM
                     IESVQGQKFSRYVPEDITTLLSMTQPLKLRGFQKWDVFCNAVNNMMNNPLLPAHGKGV
                     LVALRPVPGIRVEQALTLCRPNRTGDIMTIGGNRLVLFLSFCRINDLDTALNHIFPLP
                     TGDIFSNRMVWFEDDQISAELVQMRLLAPEQWGMPLPLTQSSKPVINAEHDGRHWRRI
                     PEPMRLLDDAVERSS"
     gene            3807..3995
                     /locus_tag="OO357_003134"
     CDS             3807..3995
                     /locus_tag="OO357_003134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjR family protein"
                     /protein_id="HCR5887987.1"
                     /translation="MNNNEPDTLPDPAIGYIFQNDIVALKQAFSLPDIDYADISQREQ
                     LAAALKRWPLLAEFAQQK"
     gene            4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO357_003135"
     CDS             4007..4759
                     /gene="bcsQ"
                     /locus_tag="OO357_003135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsQ"
                     /protein_id="HCR5887988.1"
                     /translation="MAVLGLQGVRGGVGTTTITAALAWSLQMLGENVLVVDACPDNLL
                     RLSFNVDFTHRQGWARAMLDGQDWRDAGLRYTSQLDLLPFGQLSIEEQENPQHWQTRL
                     SDICSGLQQLKASGRYQWILIDLPRDASQITHQLLSLCEHSLAIVNVDANCHIRLHQQ
                     ALPDGAHILINDFRIGSQVQDDIYQLWLQSQRRLLPMLIHRDEAMAECLAAKQPVGEY
                     RSDALAAEEILTLANWCLLNYSGLKTPVGSAS"
     gene            4756..5115
                     /locus_tag="OO357_003136"
     CDS             4756..5115
                     /locus_tag="OO357_003136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5887989.1"
                     /translation="MSILTRWLLIPPVNARLIGRYRDYRRHGASAFSATLGCFWMILA
                     WIFIPLEHPRWQRIRAEHKNLYTHINASRPRPLDPVRYLIQTCWLLIGASRKETPKPR
                     SGHFQVCKIFVDVTING"
     gene            5112..7373
                     /gene="bcsA"
                     /locus_tag="OO357_003137"
                     /pseudo
     CDS             5112..7373
                     /gene="bcsA"
                     /locus_tag="OO357_003137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077779.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-forming cellulose synthase catalytic
                     subunit"
     gene            7384..9723
                     /gene="bcsB"
                     /locus_tag="OO357_003138"
     CDS             7384..9723
                     /gene="bcsB"
                     /locus_tag="OO357_003138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis cyclic di-GMP-binding
                     regulatory protein BcsB"
                     /protein_id="HCR5887990.1"
                     /translation="MKRKLFWICAVAMGMSAFPSFMTQATPATQPLINAEPAVAAQTE
                     QNPQVGQVMSGEQGADAPIVAQNGPSRDVKLTFAQIAPPPGSMVLRGINPNGSIEFGM
                     RSDEVVTKAMLNFEYTPSPSLLPVQSQLKVYLNDELMGVLPVTKEQLGKKTLAQMPIN
                     PLFITDFNRVRLEFVGHYQDVCENPASTTLWLDVGRSSGLDLTYQTLNVKNDLSHFPV
                     PFFDPRDNRTNTLPMVFAGAPDVGLQQASAIVASWFGSRSGWRGQNFPVLYNQLPDRN
                     AIVFATNDKRPDFLRDHPAVKAPVIEMINHPQNPYVKLLVVFGRDDKDLLQAAKGIAQ
                     GNILFRGESVVVNEVKPLLPRKPYDAPNWVRTDRPVTFGELKTYEEQLQSSGLEPAAI
                     NVSLNLPPDLYLMRSTGIDMDINYRYTMPPVKDSSRMDISLNNQFLQSFNLSSKQEAN
                     RLLLRIPVLQGLLDGKTDVSIPALKLGATNQLRFDFEYMNPMPGGSVDNCITFQPVQN
                     HVVIGDDSTIDFSKYYHFIPMPDLRAFANAGFPFSRMADLSQTITVMPKAPNEAQMET
                     LLNTVGFIGAQTGFPAINLTVTDDGSTIQGKDADIMIIGGIPDKLKDDKQIDLLVQAT
                     ESWVKTPMRQTPFPGIVPDESDRAAETQSTLTSSGAMAAVIGFQSPYNDQRSVIALLA
                     DSPRGYEMLNDAVNDSGKRATMFGSVAVIRESGINSLRVGDVYYVGHLPWFERLWYAL
                     ANHPILLAVLATISVILLAWVLWRLLRIISRRRLNPDNE"
     gene            9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO357_003139"
     CDS             9730..10836
                     /gene="bcsZ"
                     /locus_tag="OO357_003139"
                     /EC_number="3.2.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase"
                     /protein_id="HCR5887991.1"
                     /translation="MNVLRSGIVTMLLLAAFSVQAACTWPAWEQFKKDYISQEGRVID
                     PSDARKITTSEGQSYGMFFALAANDRAAFDNILDWTQNNLAQGSLKERLPAWLWGKKE
                     NSKWEVLDSNSASDGDVWMAWSLLEAGRLWKEQRYTDIGSALLKRIAREEVVTVPGLG
                     SMLLPGKVGFAEDNSWRFNPSYMPPTLAQYFTRFGAPWTTLRETNQHLLLETAPKGFS
                     PDWVRYEKDKGWQLKAEKTLISSYDAIRVYMWVGMMPDSDPQKARMLNRFKPMATFTE
                     KNGYPPEKVDVATGKAQGKGPVGFSAAMLPFLQNRDAQAVQRQRVADNFPGSDTYYNY
                     VLTLFGQGWDQHRFRFSTKGELLPDWGQECANSH"
     gene            10913..11254
                     /locus_tag="OO357_003140"
     CDS             10913..11254
                     /locus_tag="OO357_003140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000377023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose synthase"
                     /protein_id="HCR5887992.1"
                     /translation="MDSGNTNAVRGLANIYRQQSPEKAEAFIASLSASQRRSIDDIER
                     SLQNDRLAQQAEVLENQGKWAQAAALQRQRLALGPGSVWITYRLAGRSCAAIQRWRQQ
                     SGLRLHGAGIT"
     gene            11169..11420
                     /locus_tag="OO357_003141"
                     /pseudo
     CDS             11169..11420
                     /locus_tag="OO357_003141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026226.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="BCSC C-terminal domain-containing protein"
     gene            11502..13490
                     /gene="hmsP"
                     /locus_tag="OO357_003142"
     CDS             11502..13490
                     /gene="hmsP"
                     /locus_tag="OO357_003142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm formation regulator HmsP"
                     /protein_id="HCR5887993.1"
                     /translation="MRVSRSLTIKQMAMVAAVVLVFVFIFCTVLLFHLVQQNRYNTAT
                     QLESIARSVREPLSSAILKGDIPEAEAILASIKPAGVVSRADVVLPNQFQALRKSFIP
                     ERPVPVMVTRLFELPVQISLGVYSLERPANPQPIAYLVLQADSFRMYKFVMSTLSTLV
                     TIYLLLSLILTVAISWCINRLILHPLRNIARELNAIPAQELVGHQLALPRLHQDDEIG
                     MLVRSYNLNQQLLQRHYEEQNENAMRFPVSDLPNKALLMEMLEQVVARKQTTALMIIT
                     CETLRDTAGVLKEAQREILLLTLVEKLKSVLSPRMILAQISGYDFAVIANGVQEPWHA
                     ITLGQQVLTIMSERLPIERIQLRPHCSIGVAMFYGDLTAEQLYSRAISAAFTARHKGK
                     NQIQFFDPQQMEAAQKRLTEESDILNALENHQFAIWLQPQVEMTSGKLVSAEVLLRIQ
                     QPDGSWDLPDGLIDRIECCGLMVTVGHWVLEESCRLLAAWQERGIMLPLSVNLSALQL
                     MHPNMVADMLELLTRYRIQPGTLILEVTESRRIDDPHAAVAILRPLRNAGVRVALDDF
                     GMGYAGLRQLQHMKSLPIDVLKIDKMFVEGLPEDSSMIAAIIMLAQSLNLQMIAEGVE
                     TEAQRDWLAKAGVGIAQGFLFARPLPIEIFEESYLEEK"
BASE COUNT         3195 a         3474 c         3716 g         3233 t
ORIGIN      
        1 aagcggaagt aacgtgtcat gcgggcgtca ggctgccgta atggcaattt gcgcccggat
       61 caggccgcag aggttaaact ctgcggcctt tttcgtgctt actgcgggta aggcacccag
      121 tcgccgccgt tcaggcgaac gtacggttta tcttggtatt gaataactac tgcatttgag
      181 ttctcggaga ccggtgctgt ttgtggcaac ccactggtga gttttttcca gtcaacattg
      241 tcttcggtga aaatcttgcc atcaagaacg cgaaccacca gatcggagat agccaggaag
      301 ctgctcggtt ggtcaatgac aatcggtgcc ccctgatgtg gtgccttcat gccgaagaat
      361 ttcaccccaa cggggacgtc tgtgatagac gggctaggga tatcacgtag gccagatacc
      421 tgcattctgt cgcccttcag cgcgccgccg tgttccggca ccacgaccac catcacttta
      481 cgacccgatt tctccagttc agtaaagaag gcgtccagtt catcaaagaa tttctgcgcc
      541 cgcgctttgt aatccgctgt tttgctgacc cccggataat ggttgccgtc atgcagtgga
      601 agcgtgttgt agaacgtggc actacggctg tttttatctt tttcggtaac gtccagccag
      661 cggttaagca cggcggtatc gtcataaacc ggcgaaccat caaagcccag caaaataacc
      721 ggcagatttg tttgatccat caattcagtc tgcatgccgc cattttcgcg aacttctttc
      781 aaaaaaccgc cgaactggcc gttatgtccc atcatcaggt gctgggtaaa gcccagtttc
      841 gaaaggttat caaacagata gcagtcgtta tttgccggtt gatacagatt agtgtgcgaa
      901 gtctgcccgc agctggcgcg cagtaaacgg atcgccgccg ggccactgta ggaggtggcg
      961 gagttaaagt tcttgaaatc aatatcgaaa tgcgaccaca gtggatgcga catcaacccg
     1021 gcggcttcta tatccgacca ggaaagcgaa cagatgttaa tcaccagtag ttcaaatggc
     1081 tgagcatcag cgggcagcga agacgggaag gtcgatttac gtttcgcctc cgcgttatag
     1141 aaattattca gccaggcgtt aaggttcgcc gttgttggcg gtgcagtttg tgcgggcata
     1201 tcacccacta ccggtgcgcc acccgtcgcc gcaacggttg ccgctgcgtt accacccgtc
     1261 gttgttacag tggtcgtcgg ttgtccggct ggccacaagg agaaacttgg tcccgccagg
     1321 gtaagtacgt tcagccatag cagtatggca accacaaaaa cggtaatgcg aatccattgt
     1381 gacaggaata accaggccac taataaaaca aaaatggccc caatcatctg ccagttaata
     1441 aagcgtgtga caaggtcgat taaataatcg gtactgaacc ccgccacctg cgaaccctgg
     1501 ctcattatgc tttccgggcc aggcaaccag atgtcatgcc agaacaaagc aaagccgatc
     1561 ggcagggcaa tccagtggcg caagcgatgc aggctgtaac gcggaatggg catcagcaga
     1621 aacgcggcaa acaccaaatt gaggagcggg tggaagttaa gatatcccgc ccacaacagg
     1681 ccaaacttaa ccagaaaata gaagttccag ccggaaaggc cgcgccagta ttgccagagg
     1741 gaagaaggca tggcggtatt ttgcgtaaat tgagtcattt tttggttgcc ctggcttttt
     1801 ccgtgcggcg taacgtcccg gccggtttaa cataacgagg tttagcaaag aacaaacgta
     1861 aggtgtcgcg aatgcgtcgc aaagagtgcc gcgccagata gcccagcggg aaaaatatca
     1921 gtgcgcaaac gataataatt tcaatgatat cgctgatggt catcatgatg agcgctccac
     1981 agcatcatct aacagtcgca tgggttctgg tattcgtcgc cagtggcgac catcgtgctc
     2041 ggcattgatg accggtttag aactttgcgt taaaggcagc ggcattcccc attgttctgg
     2101 ggcaagcagg cgcatctgca ccagctcggc actgatttga tcatcttcaa accagaccat
     2161 acggtttgag aaaatgtcgc cagtaggcaa tgggaaaata tgattcaacg cggtatccag
     2221 atcgttaatc cgacagaatg agagaaacag caccagccga ttaccgccaa tggtcatgat
     2281 atcgccggta cggttagggc gacatagcgt cagggcttgc tcaacgcgga tacccggtac
     2341 cggacgtagg gcaaccagaa cgcctttacc gtgggcaggc aagagagggt tattcatcat
     2401 gttgttgacg gcattacaga acacatccca cttctggaaa ccacgcagtt tgagcggctg
     2461 ggtcattgac agcaaggtag tgatatcttc cggtacatag cgactaaact tctgcccttg
     2521 cacgctttcg atcatcgtca gacaacggga gagtggcgca ttccacggaa taaccatatt
     2581 tgcaccgcag gccaataaca aacgttcatc ggtggcgcgc aggctagcgg tattttcccg
     2641 cacgaggatt ttcatcgcac taccgcgctg gcgacgcagg gtatgaatgc tgcgggccag
     2701 tggctcgatt tgcgcatttt gctgtaaaga aaagaccacc gtcgccgcct gagcggtacg
     2761 ggcttcattg aacaggactt cgttattgtt gaacagttgc cagtgttccg atagcggcgg
     2821 cgcaccttcc agtacagcaa cattactcag aatgcgtttt tcgtcgctgc gtggttggat
     2881 ctccgcctct tcgctttgaa ctaatgtcca gataccattt tgttgctgaa cgctaagctg
     2941 ctgacgggcg ctgacccctt tttcgttgca ccagaaggca atatccagca aatgttggtc
     3001 accctgaaaa cgcaaactgg caagaccaaa aagtgaacgg tattcctcaa gcaacaatga
     3061 aaattgttta tcgttattat ttccgggatt aattaccaaa agcgaacaat ggtttaacct
     3121 gctccattta ttcattttat ccaaccatga gcgaagccgc tcggcaggaa tattttgcca
     3181 tgcgttattt gcgcaaacaa gaataaaaag gtaattatgg ggatcaatag aacactgcaa
     3241 atcacgggcc aaatagtata gacctttttc atgatttagc attgaaaata attttatttt
     3301 ttccggccct tgagaatcat ctaattgaaa gattttcgcc ggatcgctgt ccatgctaat
     3361 gaccgcgacg tgtgcggttt cagcctggga tgcaattgtt tgattcgcca gactgatagc
     3421 atcttcatgg cgatcgacgt taaaccacca gacgccgcct gctggcatat gtcgcagctc
     3481 atcccataat gatgagatac cgatagagaa tacagggtcc acaatgtccc tcatgtcacc
     3541 attttgtctg attatcagtt tactagcgaa agctaagaaa taaacctacc attgaaatta
     3601 aaacttatca gatttagcat gtgaatcaat ttagaaggtc acaagacgtg tgattttagt
     3661 cttttcgttt ttaacttttt ctgttgcgta acggaaagtc aaaaagtgag caaattcccg
     3721 tctcgccgta aacaataaac cggcgaaatg ctcacggtta gaattgtctc atgaatggta
     3781 cggttatttc atagggatca agcaaaatga ataacaatga accagatact ctgcctgatc
     3841 ccgcgatagg ctatatcttc cagaatgata ttgtggcgtt aaagcaggca ttttcactgc
     3901 ctgatattga ttatgccgat atttcccaac gcgaacagtt ggccgcggca ttaaaacgct
     3961 ggccgttgct ggcagagttt gcgcaacaaa agtaggggat tggtgaatgg ccgtactggg
     4021 attgcagggg gtgcggggag gcgtggggac aacaaccatc accgccgcat tagcctggtc
     4081 attacaaatg ttgggagaaa atgtcctggt ggtcgatgcc tgcccggaca acttgttgcg
     4141 cctgtcattt aatgttgatt ttacccaccg tcagggctgg gccagagcga tgctggatgg
     4201 ccaggactgg cgtgacgctg ggttgcgcta cacctcgcag ctcgatttgc tgccttttgg
     4261 tcagttatcc attgaagaac aagaaaatcc acagcactgg caaacccggc tgagcgatat
     4321 ttgctccggc ttacagcaac taaaagccag cgggcgttac cagtggattt taatcgactt
     4381 accgcgtgat gcctcgcaga taacccacca gctgctgagt ttgtgcgagc actcgctggc
     4441 aatcgtcaat gtggatgcca actgccatat ccgactgcat cagcaagcgc tgccggatgg
     4501 cgcacatatt ttgattaatg acttccgtat tggcagtcag gttcaggacg atatttacca
     4561 gctttggttg caaagccagc gccgattact gccgatgctc attcatcgtg atgaagcgat
     4621 ggctgaatgc ctggcggcta agcaaccagt aggtgaatat cgcagtgatg cgctggcggc
     4681 tgaagagata ctgacgctgg cgaactggtg cctgttgaac tactccgggc tgaaaacgcc
     4741 agtcgggagt gcatcatgag tatcctgacc cggtggttgc ttatcccgcc ggtcaacgcg
     4801 cggcttatcg ggcgttatcg cgattatcgt cgtcacggtg cgtcggcttt cagcgcgacg
     4861 ctcggctgtt tctggatgat cctggcctgg atttttattc cgctggagca cccgcgctgg
     4921 cagcgtattc gcgcagaaca taaaaacctg tatacgcata tcaacgcctc gcgtccgcgt
     4981 ccgctggacc cggtccgtta tctcattcaa acatgctggt tattgatcgg tgcatcgcgc
     5041 aaagaaacgc cgaaaccgcg cagcgggcat tttcaggtct gcaaaatatt cgtggacgtt
     5101 accatcaatg gatgaacgag ctgcctgagc gcgttagcca taaaacacag catctggatg
     5161 aaaaaaaaga gctcggtcat ttgagtgccg gggcgcggcg gttgatcctc ggtatcatcg
     5221 tcaccttctc gctgattctg gcgttaatct gcgttactca gccgtttaac ccgctggcgc
     5281 agtttatctt cctgatgctg ctgtgggggg tagcgctgat cgtacggcgg atgccggggc
     5341 gcttctcggc gctaatgttg attgtgctgt cgctgaccgt ttcttgccgt tatatctggt
     5401 ggcgttacac ctctacgctg aactgggacg atccggtcag cctggtgtgc gggcttattc
     5461 tgctcttcgc tgaaacgtac gcgtggattg tgctggtgct cggctacttc caggtagtat
     5521 ggccgctgaa tcgtcagccg gtgccattgc cgaaggatat gtcgctgtgg ccgtcagtgg
     5581 atatctttgt cccgacttac aacgaagatc tcaatgtggt gaaaaatacc atttacgcct
     5641 cgctgggtat cgactggccg aaagacaaac tgaacatctg gatcctcgat gacggcggca
     5701 gggaagagtt tcgccagttt gcgcaaaacg tgggggtgaa atatatcgcc cgcaccactc
     5761 atgaacatgc gaaagcgggc aacatcaaca atgcgctgaa atatgccaaa ggcgagttcg
     5821 tgtcgatttt cgactgcgac cacgtaccaa cgcgatcgtt cctgcaaatg accatgggct
     5881 ggttcctgaa agagaaacag ctggcgatga tgcagactcc acaccacttc ttctcgccgg
     5941 acccgtttga acgcaacctg ggacgttttc gtaaaacgcc gaacgaaggc acgctgttct
     6001 atggtctggt gcaggatggc aacgatatgt gggacgccac tttcttctgc ggttcctgtg
     6061 cggtgattcg tcgtaagccg ctggatgaaa ttggcggcat tgctgtcgaa accgtgactg
     6121 aagatgcgca tacttctctg cgtttgcacc gtcgtggcta tacctccgcg tatatgcgta
     6181 ttccgcaggc ggcggggctg gcgaccgaaa gtctgtcggc gcatatcggt cagcgtattc
     6241 gctgggcgcg cgggatggta taaatcttcc gtctcgataa cccgctcacc ggtaaaggac
     6301 tgaagtttgc tcagcggctg tgctacgtca acgccatgtt ccacttcttg tcgggcattc
     6361 cacggctgat cttcctgact gcgccgctgg cgttcctgct gcttcatgcc tacatcatct
     6421 atgcgccagc gttgatgatc gccctattcg tgctgccgca tatgatccat gccagcctga
     6481 ccaactcgaa gatccagggc aaatatcgcc actctttctg gagtgaaatc tacgaaacgg
     6541 tgctggcgtg gtatatcgca ccaccgacgc tggtggcgct gattaacccg cacaaaggca
     6601 aatttaacgt caccgccaaa ggtggactgg tggaagaaga gtacgtcgac tgggtgatct
     6661 cgcggcccta catcttcctt gttctactca acctggtggg cgttgcggta ggcatctggc
     6721 gctacttcta tggcccgcca accgagatgc tcaccgtggt cgtcagtatg gtgtgggtgt
     6781 tctacaacct gattgttctt ggcggcgcag ttgcggtatc ggtagaaagc aaacaggtac
     6841 gccgatcgaa ccgcgtggag atgacgatgc ccgcggcaat tgcccgcgaa gatggtcacc
     6901 tcttctcgtg taccgttcag gatttctccg acggtggttt ggggatcaag atcaacggtc
     6961 aggcgcagat tctggaaggg cagaaagtga atctgttgct taaacgcggt cagcaggaat
     7021 acgtcttccc gacccaggtg gcgcgcgtga tgggtaatga agttgggctg aaattaatgc
     7081 cgctcaccac ccagcaacat atcgattttg tgcagtgtac gtttgcccgt gcggatacat
     7141 gggcgctctg gcaggacagc tacccggaag ataagccgct ggaaagtctg ctggatattc
     7201 tgaagctcgg cttccgtggc taccgccatc tggcggagtt tgcgccttct tcggtgaagg
     7261 gcatattccg tgtgctgact tctctggttt cctgggttgt atcgtttatt ccgcgccgcc
     7321 cggagcggag cgaaacggca caaccatcgg atcaggcttt ggctcaacaa tgatgataac
     7381 gcgatgaaaa gaaaactatt ctggatttgt gcagtggcta tggggatgag tgcgttcccc
     7441 tctttcatga cgcaggcgac gccagcaacg caaccactga tcaatgctga gccagctgta
     7501 gccgcccaga cggaacaaaa tccgcaggtg gggcaagtga tgtcaggcga gcagggagct
     7561 gatgccccca tcgtggcgca gaacggtcct tcgcgtgatg tgaagctgac ctttgcgcaa
     7621 atcgcgccgc cgccgggcag catggtgcta cgtggtatta acccgaacgg cagcattgag
     7681 tttggtatgc gcagcgatga agtggtgacg aaggcgatgc tcaacttcga atacacgcca
     7741 tcgccatcgt tactgcctgt ccagtcgcag ttaaaggttt atctcaatga tgagctgatg
     7801 ggcgtgctgc cagtgaccaa agaacagttg ggtaaaaaaa cgctggcgca aatgcccatt
     7861 aatccactgt ttattaccga cttcaaccgt gtgcggctgg agtttgtcgg ccattatcag
     7921 gacgtgtgcg aaaacccggc cagcaccacg ctttggctgg atgttgggcg gagcagtgga
     7981 ctggatctga cctatcagac cctgaatgtg aagaatgacc tgtcacactt cccggtgcca
     8041 ttctttgacc cgcgcgataa ccgtactaac accttgccga tggtctttgc gggtgcgccg
     8101 gatgttgggc tgcaacaagc atctgccatt gtcgcctcgt ggtttggttc gcgttctggc
     8161 tggcgtgggc agaacttccc ggtgctctat aaccaattgc cggatcgcaa tgccattgtc
     8221 tttgcaacca acgacaaacg gccggacttc ctgcgcgatc atccggcggt aaaagccccg
     8281 gtgattgaga tgattaacca tccgcagaat ccttacgtca aactgctggt ggtgtttggt
     8341 cgtgacgaca aagacctgtt gcaggcagcg aaaggtatcg ctcagggtaa cattctgttc
     8401 cgtggtgaaa gcgtggtagt gaatgaagtg aaaccgctgc taccgcgtaa gccgtacgat
     8461 gcgccgaact gggtacgtac cgatcgtccg gtcacatttg gcgaactgaa aacctatgaa
     8521 gaacagttac aatccagcgg ccttgagcca gcagcgatta acgtttcgct aaacctgccg
     8581 ccggatctct acctgatgcg cagtaccggc attgatatgg atattaatta ccgctacacc
     8641 atgccgccgg tgaaagacag ttcgcggatg gatatcagcc tgaataacca gttcctgcaa
     8701 tccttcaacc tgagcagcaa acaggaggcg aaccgtctgc tgctgcgtat tccggtatta
     8761 caaggtttgc tggatggcaa aacagatgtc tctattccgg cgctgaaact gggcgcgacc
     8821 aaccagctgc gcttcgactt tgagtatatg aacccgatgc caggcggttc ggtagataac
     8881 tgtattacct tccagccggt gcagaatcat gtggtgattg gtgacgattc caccatcgac
     8941 ttctcgaagt attaccactt catcccgatg ccggatctac gcgcctttgc taacgcgggc
     9001 ttcccattca gccggatggc ggatctgtcg caaaccatca ccgtgatgcc gaaagcgcct
     9061 aacgaagcac agatggaaac gttgctgaat actgttggtt ttatcggcgc acagacgggc
     9121 ttcccggcga ttaatctgac ggtgaccgat gatggcagca ccattcaggg caaagatgcc
     9181 gacatcatga tcatcggtgg tatcccggac aaactgaaag acgataagca gatcgaccta
     9241 ttggtgcagg cgaccgaaag ctgggtgaaa acaccgatgc gccagacccc gttccccggc
     9301 attgtgccgg acgagagcga tcgcgcggca gaaacccagt caacgctgac ctcttccggt
     9361 gcgatggcgg cggtgattgg cttccagtcg ccgtataacg accagcgcag cgtgattgcg
     9421 ctgttggcag atagcccacg cggttatgaa atgcttaacg atgcggtgaa cgatagcggc
     9481 aaacgcgcca ccatgttcgg ttcggtcgcg gtgatccgcg agtccggtat caacagccta
     9541 cgtgttggcg acgtttatta cgtaggtcat ctgccgtggt tcgagcgctt gtggtatgcg
     9601 ctggcaaacc atccgattct gctggcggtg ctggcgacta tcagtgtgat attgctggca
     9661 tgggtactgt ggcgtctgct gcgaattatt agtcgtcgtc gtcttaaccc ggataacgag
     9721 taattgaaga tgaatgtgtt gcgtagtgga atcgtgacga tgctgttgtt ggctgccttt
     9781 agtgttcagg cagcctgtac ctggcctgcc tgggagcagt ttaaaaagga ttacatcagt
     9841 caggaagggc gcgtcatcga ccccagcgac gcgcgcaaaa tcaccacctc cgaagggcaa
     9901 agttacggta tgttctttgc cctggcggct aacgaccgtg cagctttcga taatattctc
     9961 gactggacgc agaacaatct cgctcagggt tctttaaaag aacgtttgcc cgcctggctg
    10021 tggggcaaga aagagaacag taagtgggaa gtgctggaca gcaattcggc ctccgatggt
    10081 gatgtctgga tggcctggtc gttgctggag gcggggcgtt tgtggaaaga gcagcgttat
    10141 accgacatcg gcagcgcgtt gctaaaacgt atcgcgcggg aggaagtggt gacggtgcct
    10201 gggctgggtt ccatgttgtt accgggcaaa gtgggttttg ctgaggataa cagctggcgt
    10261 tttaacccca gctacatgcc gccgacgctg gcgcagtatt tcacccgctt tggcgcgccg
    10321 tggaccacgc tgcgcgaaac caatcaacat ttattgctgg aaaccgcccc gaaaggcttt
    10381 tcgccagact gggtgcgcta tgagaaagac aaaggctggc agctaaaagc cgaaaaaaca
    10441 ttgatcagca gctacgacgc tatccgcgtt tacatgtggg taggcatgat gcctgacagc
    10501 gatccgcaaa aagcgcggat gctcaaccgg tttaaaccga tggcgacatt cactgagaaa
    10561 aacggttatc cgccggaaaa agtggatgtg gctacgggga aagcgcaggg taaaggaccg
    10621 gtcggttttt ctgccgccat gctgcctttt ttacaaaacc gcgatgcgca ggcagttcag
    10681 cgccagcgcg tggccgataa ctttcccggc agcgatacct attacaacta tgtgctgacc
    10741 ctgtttggac aaggctggga tcaacaccgt ttccgcttct cgacaaaagg tgagttatta
    10801 cctgactggg gccaggaatg cgcaaattca cactaaacat attcacgctt tccctcggtc
    10861 tggccgtcat gccgatggtc gaggcagcac caaccatcag cagaccctgc ggatggacag
    10921 cggcaacact aatgccgtgc gcgggctggc gaatatttac cgccagcagt cgccagaaaa
    10981 agctgaagcg tttatcgcct cgctctctgc cagccagcgg cgcagcattg atgatatcga
    11041 acgcagcctg caaaacgacc gtctggcaca gcaggcagag gtactggaaa accagggcaa
    11101 atgggcgcag gcggcagcac ttcagcggca acgactggcg ctgggccccg gcagcgtatg
    11161 gattacttac cgactggcag gaagaagctg cgcggcaatc caacgatggc ggcagcagtc
    11221 agggcttcgg ctacacggcg cgggcattac ttgaacgacg tgttacttcc aactggtttg
    11281 ttggcacggc aattgatatc cagcaggtga aagattacgc acccagccat ttcctgctct
    11341 acgtacgtta ttccgccgcc ggatggcagg gtgacatgga tttaccgccg cagccgctga
    11401 taccttacgc cgactggtaa gttttcagat agcgcctctc ttaatgccgc tgcgatcggg
    11461 tatactcggg cggcaatctg ggatttccgg ggggagacaa tttgcgcgta agtcgctcgt
    11521 taacaatcaa gcagatggca atggtggcag ccgttgtcct ggtgttcgtt tttatttttt
    11581 gcaccgtttt gctgttccat ctggtccagc agaatcgcta taacacggct acgcaactgg
    11641 aaagcattgc tcgctctgtc cgcgaaccct tatcttcagc tattttgaaa ggcgatattc
    11701 ccgaagcgga agctattctt gccagcatta aaccggcagg cgtggtcagc cgtgccgatg
    11761 tagtgctgcc taaccagttc caggcgctgc gtaaaagttt tattccagag cgtccggtgc
    11821 cggtaatggt tactcgcctg tttgagctac cggttcaaat ctcgctgggc gtctactcgc
    11881 tcgaacgtcc ggcaaatccg cagccaatag cctatctggt gctacaggcg gattccttcc
    11941 gtatgtataa gttcgtgatg agcactctct caacgttagt gaccatttac ttacttttgt
    12001 cgcttatcct gaccgtcgcc atcagctggt gcattaaccg cctgattttg catccgttac
    12061 gcaatattgc tcgcgaactt aacgccatcc cagcccagga gcttgttggt caccaactgg
    12121 cattaccgcg tctgcatcag gacgatgaaa tcggtatgtt ggtgcgcagt tacaacctca
    12181 accagcaatt gctgcagcgc cattatgaag aacagaacga aaatgcgatg cgcttcccgg
    12241 tgtcggattt gccgaacaaa gccttgctga tggagatgct ggagcaggtt gtcgcgcgta
    12301 aacaaaccac cgcgctgatg atcatcacct gtgaaaccct gcgtgatact gcgggcgtgc
    12361 tgaaagaggc gcaacgagaa attctgctgc tgacgctggt ggaaaaactc aaatcggtac
    12421 tgtcgccacg tatgatcctc gcgcagatta gcggttatga ctttgctgtc attgccaacg
    12481 gtgtacagga accgtggcac gcaatcactt tgggtcagca agtactcact atcatgagcg
    12541 agcgcctgcc gattgaacgt attcaactcc gtccgcactg tagcattggc gtggcgatgt
    12601 tctacggcga tctcaccgcc gaacagcttt acagtcgcgc tatttctgcg gcatttaccg
    12661 ctcgccataa aggcaagaat cagattcagt tctttgatcc gcagcagatg gaagcagccc
    12721 agaagcggtt gacggaagag agcgatatcc ttaatgcact ggaaaatcat cagtttgcaa
    12781 tttggttaca gccacaggtc gagatgacca gcggtaaact ggtcagtgcg gaagtgttac
    12841 tgcgtatcca gcaaccggat ggcagttggg acctgccgga tggcttaatc gatcgcattg
    12901 agtgctgtgg gctgatggtt accgtcggtc actgggtgct ggaagagtcc tgtcgattgc
    12961 ttgcagcctg gcaagagcgc ggcattatgc tgcccttgtc ggtaaacctc tctgcgctgc
    13021 aactgatgca cccgaatatg gtggcggata tgctggaact gttaacccgc tatcgcattc
    13081 agccgggaac actgattctg gaagtgacag aaagccgacg tattgacgac cctcatgctg
    13141 cggtggcaat cctccgtccg ctgcgcaatg ccggagttcg ggtggcgctg gatgatttcg
    13201 gcatgggcta cgcagggctg cgtcagctgc agcatatgaa atcgttgcca atcgacgtac
    13261 tgaaaatcga caaaatgttt gttgaaggct tgccggaaga tagcagcatg attgctgcaa
    13321 ttatcatgct ggcgcagagc ctgaacttac aaatgattgc cgaaggcgtg gagactgaag
    13381 cacaacgaga ctggctggca aaagcgggcg ttggtattgc ccagggcttc ctttttgctc
    13441 gcccactccc tattgaaatc ttcgaagaga gttacctgga agaaaagtag ctaccccaaa
    13501 ctgattacaa aactttaaaa agtgctggtt tgtgcgagcc agctcaaact ttttaacctt
    13561 tttgtttcaa ttatgatcca ggtacatttc tgtgatgttg tctgggtgtt attttaag
//
LOCUS       DAJTRC010000095        13314 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.095, whole genome shotgun sequence.
ACCESSION   DAJTRC010000095 DAJTRC010000000
VERSION     DAJTRC010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13314)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13314)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13314
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.095"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            103..1950
                     /gene="ldtD"
                     /locus_tag="OO357_003143"
     CDS             103..1950
                     /gene="ldtD"
                     /locus_tag="OO357_003143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5887994.1"
                     /translation="MLLNMMCGRRLSAISLCLAVTFAPLFNAQADEPEVIPGDSPVAV
                     SEQGEALPQAQATAIMAGIQPLPEGAAEKARTQIESQLPAGYKPVYLNQLQLLYAARD
                     MQPMWENRDAVKAFQQQLAEVAIAGFQPQFNKWVELLTDPGVNGMARDVVLSDAMMGY
                     LHFIANIPIKGTRWLYSSKPYALSTPPLSVINQWQLALDKGQLPTFVAGLAPQHPQYA
                     AMHESLLVLLSDTKPWPQLTGKATLRPGQWSNDVPALREILQRTGMLDGGPKITLPGD
                     DTPTDAVVSPSAVTVETAETKPMDKQTTSRSKPAPAVRAAYDNELVEAVKRFQAWQGL
                     GADGAIGPATRDWLNVTPAQRAGVLALNIQRLRLLPTELSTGIMVNIPAYSLLYYQNG
                     NQVLDSRVIVGRPDRKTPMMSSALNNVVVNPPWNVPPTLARKDILPKVRNDPGYLESH
                     GYTVMRGWNSREAIDPWEVDWSTITASNLPFRFQQAPGPRNSLGRYKFNMPSSEAIYL
                     HDTPNHNLFKRDTRALSSGCVRVNKASDLANMLLQDAGWNDKRISDALKQGDTRYVNI
                     RQSIPVNLYYLTAFVGADDRTQYRTDIYNYDLPARSSSQIVSKAEQLIR"
     gene            2131..2679
                     /locus_tag="OO357_003144"
     CDS             2131..2679
                     /locus_tag="OO357_003144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcbK family protein"
                     /protein_id="HCR5887995.1"
                     /translation="MDKFDANRRKLLALGGVALGAAILPTPAFATLSTPRPRILTLNN
                     LHTGESIKAEFFDGRGYIQEELAKLNHFFRDYRANKIKSIDPGLFDQLYRLQGLLGTR
                     KPVQLISGYRSIDTNNELRARSRGVAKKSYHTKGQAMDFHIEGIALSNIRKAALSMRA
                     GGVGYYPRSNFVHIDTGPARHW"
     gene            2706..3353
                     /gene="gloC"
                     /locus_tag="OO357_003145"
     CDS             2706..3353
                     /gene="gloC"
                     /locus_tag="OO357_003145"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003035823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase GloC"
                     /protein_id="HCR5887996.1"
                     /translation="MNYRIIPVTAFSQNCSLIWCEQTRLAALVDPGGDAEKIKQEVDD
                     SGLTLMQILLTHGHLDHVGAAAELAQHYGVPVFGPEKEDEFWLQGLPAQSRMFGLEEC
                     QPLTPDRWLNEGDTISIGNVTLQVLHCPGHTPGHVVFFDDRAKLLISGDVIFKGGVGR
                     SDFPRGDHNQLISSIKDKLLPLGDDVTFIPGHGPLSTLGYERLHNPFLQDEMPVW"
     gene            complement(3576..4766)
                     /gene="aspC"
                     /locus_tag="OO357_003146"
     CDS             complement(3576..4766)
                     /gene="aspC"
                     /locus_tag="OO357_003146"
                     /EC_number="2.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009653304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate transaminase"
                     /protein_id="HCR5887997.1"
                     /translation="MFENITAAPADPILGLADLFRADERPGKINLGIGVYKDETGKTP
                     VLTSVKKAEQYLLENETTKNYLGIDGILEFGRCTQELLFGKGSALINDKRARTAQTPG
                     GTGALRVAADFLAKNTSVKRVWVSNPSWPNHKSVFNSAGLEVREYAYYDAENHTLDFD
                     ALINSLNEAQAGDVVLFHGCCHNPTGIDPTLEQWQTLAQLSVEKGWLPLFDFAYQGFA
                     RGLEEDAEGLRAFAAMHKELIVASSYSKNFGLYNERVGACTLVAADSEIVDRAFSQMK
                     AAIRANYSNPPAHGASVVATILSSDALRAIWEQELTDMRQRIQRMRQLFVNTLQEKGA
                     NRDFSFIIKQNGMFSFSGLTKEQVLRLREEFGVYAVASGRVNVAGMTPDNMAPLCESI
                     VAVL"
     gene            complement(4951..6039)
                     /gene="ompF"
                     /locus_tag="OO357_003147"
     CDS             complement(4951..6039)
                     /gene="ompF"
                     /locus_tag="OO357_003147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpF"
                     /protein_id="HCR5887998.1"
                     /translation="MMKRNILAVIVPALLVAGTANAAEIYNKDGNKVDLYGKTVGLHY
                     FSKGNGENSYGGNGDMTYARLGFKGETQINSDLTGYGQWEYNFQGNNSEGADAQTGNK
                     TRLAFAGLKYADVGSFDYGRNYGVVYDALGYTDMLPEFGGDTAYSDDFFVGRVGGVAT
                     YRNSNFFGLVDGLNFAVQYLGKNERDTARRSNGDGVGGSISYEYEGFGIVGAYGAADR
                     TNLQEAQPLGNGKKAEQWATGLKYDANNIYLAANYGETRNATPITNKFTNTSGFANKT
                     QDVLLVAQYQFDFGLRPSIAYTKSKAKDVEGIGDVDLVNYFEVGATYYFNKNMSTYVD
                     YIINQIDSDNKLGVGSDDTVAVGIVYQF"
     gene            complement(6640..8040)
                     /gene="asnS"
                     /locus_tag="OO357_003148"
     CDS             complement(6640..8040)
                     /gene="asnS"
                     /locus_tag="OO357_003148"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005123540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HCR5887999.1"
                     /translation="MSVVPVADVLQGRVAVDSEVTVRGWVRTRRDSKAGISFLAVYDG
                     SCFDPVQAVINNSLPNYNEDVLRLTTGCSVIVTGKVVASPGQGQQFEIQASKVEVAGW
                     VEDPDTYPMAAKRHSIEYLREVAHLRPRTNLIGAVARVRHTLAQALHRFFNEQGFFWV
                     STPLITASDTEGAGEMFRVSTLDLENLPRNDQGKVDFDKDFFGKESFLTVSGQLNGET
                     YACALSKIYTFGPTFRAENSNTSRHLAEFWMLEPEVAFANLNDIAGLAEAMLKYVFKA
                     VLEERADDMKFFAERVDKDAVSRLERFIEADFAQVDYTDAVTILENCGRKFENPVYWG
                     VDLSSEHERYLAEEHFKAPVVVKNYPKDIKAFYMRLNEDGKTVAAMDVLAPGIGEIIG
                     GSQREERLDVLDERMLEMGLNKEDYWWYRDLRRYGTVPHSGFGLGFERLIAYVTGVQN
                     VRDVIPFPRTPRNASF"
     gene            complement(8209..9411)
                     /gene="pncB"
                     /locus_tag="OO357_003149"
     CDS             complement(8209..9411)
                     /gene="pncB"
                     /locus_tag="OO357_003149"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HCR5888000.1"
                     /translation="MTQFASPVLHSLLDTDAYKLHMQQAVFHHYYDVHVAAEFRCRGD
                     DLLGIYADAIREQIQAMQHLRLQDDEYQWLSALPFFKADYLNWLREFRFNPEQVTVSN
                     DNGKLDIRLSGPWREVILWEVPLLAVISEMVHRYRSPQADVAQALDTLESKLVDFSAL
                     TAGLDMSRFHLMDFGTRRRFSREVQETIVKRLQQESWFVGTSNYDLARRLSLTPMGTQ
                     AHEWFQAHQQISPDLANSQRAALAAWLEEYPDQLGIALTDCITMDAFLRDFGVEFASR
                     YQGLRHDSGDPVEWGEKAIAHYEKLGIDPQSKTLVFSDNLDLRKAVELYRHFSSRVQL
                     SFGIGTRLTCDIPQVKPLNIVIKLVECNGKPVAKLSDSPGKTICHDKAFVRALRKAFD
                     LPHIKKAS"
     gene            9677..12292
                     /gene="pepN"
                     /locus_tag="OO357_003150"
     CDS             9677..12292
                     /gene="pepN"
                     /locus_tag="OO357_003150"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000193787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HCR5888001.1"
                     /translation="MTQQPQAKYRHDYRAPDYQITDIDLTFDLDAQKTVVTAVSQAVR
                     HGASDAPLRLNGEDLKLVSVHINDEPWTAWKEEEEGALVISNLPERFTLKIINEISLA
                     ANTALEGLYQSGDALCTQCEAEGFRHITYYLDRPDVLARFTTKIIADKIKYPFLLSNG
                     NRVAQGELENGRHWVQWQDPFPKPCYLFALVAGDFDVLRDTFTTRSGREVALELYVDR
                     GNLDRAPWAMTSLKNSMKWDEERFGLEYDLDIYMIVAVDFFNMGAMENKGLNIFNSKY
                     VLARTDTATDKDYLDIERVIGHEYFHNWTGNRVTCRDWFQLSLKEGLTVFRDQEFSSD
                     LGSRAVNRINNVRTMRGLQFAEDASPMAHPIRPDMVIEMNNFYTLTVYEKGAEVIRMI
                     HTLLGEENFQKGMQLYFERHDGSAATCDDFVQAMEDASNVDLSHFRRWYSQSGTPVVT
                     VKDDYNPETEQYTLTISQRTPATPDQAEKQPLHIPFAIELYDNEGKVIPLQKGGHPVN
                     SVLNVTQAEQTFVFDNVYFQPVPALLCEFSAPVKLEYKWSDQQLTFLMRHARNGFSRW
                     DAAQSLLATYIKLNVARHQQGQPLSLPVHVADAFRAVLLDEKIDPALAAEILTLPSVN
                     EMAELFDIIDPIAIAEVREALTRTLATELADELLAIYNANYQSEYRVEHEDIAKRTLR
                     NACLRFLAFGETHLADVLVSKQYHEANNMTDALAALSAAVAAQLPCRDALMQEYDDKW
                     HQDGLVMDKWFILQATSPAANVLETVRGLLQHRSFTMSNPNRIRSLIGAFAGSNPAAF
                     HAEDGSGYQFLVEMLTDLNSRNPQVASRLIEPLIRLKRYDAKRQEKMRAALEQLKGLE
                     NLSGDLYEKITKALA"
     gene            complement(12499..13266)
                     /gene="ssuB"
                     /locus_tag="OO357_003151"
     CDS             complement(12499..13266)
                     /gene="ssuB"
                     /locus_tag="OO357_003151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonates ABC transporter ATP-binding
                     protein"
                     /protein_id="HCR5888002.1"
                     /translation="MNTARLNQGTPLLLNAVSKHYAENIVLNQLDLHIPAGQFVAVVG
                     RSGGGKSTLLHLLAGLETPTAGDVLAGTTPLAEIQDDTRMMFQDARLLPWKSVIDNVG
                     LGLKGQWRDAARRALAAAGLENRAGEWPAALSGGQKQRVALARALIHRPGLLLLDEPL
                     GALDALTRLEMQDLIVSLWQQHGFTVLLVTHDVSEAVAMADRVLLIEEGKISLDLTVD
                     IPRPRRLGSVRLAELEAEVLQRVMRRGHSEQLIRRHG"
BASE COUNT         3363 a         3381 c         3374 g         3196 t
ORIGIN      
        1 acaggcttgc tcgtcatact ttcgtgagta ttggcgttgt acaggcaagt cgtaaaataa
       61 cagcctggct attcagagta tgataaaaac agggggcaag ggatgttgct taatatgatg
      121 tgtggtcgtc ggctgtcggc aatcagtttg tgcctggccg taacattcgc tccactgttc
      181 aatgcgcagg ccgatgagcc tgaagtaatc cctggcgaca gcccggtggc tgtcagtgaa
      241 cagggcgagg cactgccgca ggcgcaagcc acggcaataa tggcggggat ccagccattg
      301 cctgaaggtg cggcagaaaa agcccgcacg caaatcgaat ctcaattacc cgcaggttac
      361 aagccggttt atcttaacca gcttcaactg ttgtatgccg cacgcgatat gcaacccatg
      421 tgggaaaacc gtgatgctgt taaagccttc cagcaacagc tggcagaggt ggcgattgct
      481 ggtttccagc cgcagtttaa taaatgggta gagttactga ccgatcctgg tgttaacggg
      541 atggcacgtg acgtggtgct ctctgatgcg atgatgggct atctccattt cattgcaaat
      601 attccgatca aaggcactcg ctggctatat agcagtaaac cttatgcgct ttcaacgccg
      661 ccgctttcgg tgattaacca atggcagcta gcgctggata aaggtcaatt gcctacgttt
      721 gttgcaggac tggcaccgca gcatccgcaa tatgcggcga tgcatgaatc gttactggtc
      781 ttactcagtg acaccaaacc gtggccccaa ctgaccggca aagcaacgtt gcgcccaggg
      841 cagtggagta acgacgtacc ggcgttgcgc gaaatattgc aacgcacagg catgttggac
      901 ggggggccga aaattactct acctggcgat gacacgccaa ctgacgcggt agtcagccca
      961 tccgctgtta ctgttgaaac agcagaaact aagccgatgg acaagcaaac gacgtctcgt
     1021 agtaaacctg cgcctgccgt tcgtgccgcc tacgataatg aactggtgga agccgttaaa
     1081 cgttttcagg catggcaagg attgggagca gatggtgcta ttggcccggc aacgcgtgac
     1141 tggttaaacg taacgcccgc ccagcgtgct ggcgtgttgg cactcaacat ccagcgattg
     1201 cgcttgctgc caacagagct ttctaccggg atcatggtta acattccagc ctattcgctg
     1261 ctctactatc agaacggcaa tcaggtgctg gattcgcgag tcattgtcgg tcgccccgat
     1321 cgcaaaacgc cgatgatgag cagtgcactt aacaacgtag tggtaaaccc gccgtggaac
     1381 gtaccaccaa ctctggcacg caaagatatt ctgccgaaag tgcgcaacga tccgggatat
     1441 ctcgaaagcc atggctatac ggtgatgcgc ggctggaaca gcagagaagc gattgaccca
     1501 tgggaggttg actggtctac aatcacggcc tcgaatttac cgtttcgctt ccagcaggct
     1561 ccaggcccac ggaactcgct ggggcgctat aaattcaata tgccgagttc agaggccatt
     1621 tatttgcatg acacgccgaa ccacaatctg ttcaagcgtg atacacgcgc attgagctca
     1681 ggctgtgtac gagtgaataa agcttccgat ctggcgaata tgctgttgca ggatgcagga
     1741 tggaatgaca aacgtatttc tgatgcgctg aagcagggtg atacacgtta cgtcaatatt
     1801 cggcagtcga ttccggtgaa tctctactac ctgacggcct ttgttggtgc agatgatcgt
     1861 acccagtatc gtacagatat ttacaattat gatctgcctg cgcgatccag ctcgcaaatc
     1921 gtatcgaaag cggaacaatt aatcaggtaa atgaagtagt tcggggaaat caattgtcgt
     1981 aataattagc atgattgggg gcgattctct gcagcccccg tcactgctgg ggttgagtca
     2041 tcttgacgtc tgctttacgg gcggttaagg tgcctcttgt gcgccagaag tgcatataaa
     2101 cgataacatt gagctgtaga cttgattatc atggacaaat tcgacgctaa tcgccgcaaa
     2161 ttgctggcgc ttggtggcgt tgcactcggt gccgccatcc tgccgacccc tgcgtttgca
     2221 acactctcta ccccacgccc gcgcattttg acactcaata atcttcatac cggagagtca
     2281 atcaaagcgg agtttttcga tggcagaggc tatattcagg aagaattggc aaaacttaac
     2341 cattttttcc gcgattaccg cgcgaacaaa ataaagtcca tcgacccagg attattcgac
     2401 cagttgtatc gcctgcaagg gttgttaggc acgcgcaaac cggtgcaact catttccggt
     2461 tatcgttcca ttgataccaa caatgaacta cgcgcccgca gccgtggagt agcgaagaaa
     2521 agctatcaca ctaaaggcca ggcgatggat ttccatattg aaggtatcgc gttaagcaat
     2581 attcgcaaag ccgcgttatc tatgcgcgca ggtggtgtag gatattaccc acgtagtaac
     2641 tttgtgcata ttgataccgg gccagcacgg cactggtagt aatcgcttaa cgaaacaggg
     2701 gcagtatgaa ctatcgtatt attccggtca ccgcattctc ccagaactgt tcattgatct
     2761 ggtgtgaaca aacccgcctg gccgcactgg tcgatcctgg cggcgatgcg gaaaaaatca
     2821 aacaggaagt tgatgacagc ggcctgacac tgatgcagat cctgctgacg catggtcatc
     2881 tggaccacgt tggcgcagcg gcggaactgg cgcaacatta cggcgtgccg gtttttggcc
     2941 cggaaaaaga agatgagttc tggctgcaag gcttgcctgc gcaaagtcgt atgtttggtc
     3001 tggaagagtg ccagccgctg acgccagatc gttggctgaa cgaaggcgat accatcagca
     3061 tagggaatgt gactttacag gtgttacatt gccctgggca tacgccgggt catgtcgtgt
     3121 tttttgatga tcgggcaaag ctgctgattt ctggcgatgt tattttcaaa ggcggagtag
     3181 ggcgcagtga cttcccgcgt ggcgatcata atcaactgat ttcttcaatc aaagataaat
     3241 tgctgccact gggggatgac gtgacattta ttccgggtca cggaccatta tctacacttg
     3301 gttatgagcg cctgcacaat cccttcctgc aagacgaaat gcccgtctgg taaaggcgca
     3361 taaaaaagtc cacttttaat gcgggcctgg atttctggca aagtgcgctt tgtttatgcc
     3421 ggatgcggca cgagcgcctt atccggccta caaaatcgtg caaattcaaa atattgcagg
     3481 ggacgcgtag gcctgataag cgtagcgcat caggcaatgt tgcgtttgtc atcagtctca
     3541 gcccgctttt cagcgggctt cattgttttt aacgcttaca gcactgccac aatcgattcg
     3601 cacagcggag ccatgttatc tggtgtcatc ccggccacat ttacgcgacc agaagcaaca
     3661 gcatatacgc caaactcttc gcgcagacgc agcacttgtt cttttgtcag accactgaag
     3721 gagaacatgc cgttctgttt gatgataaag ctgaagtcgc ggtttgcacc tttttcctgc
     3781 agcgtattga cgaacaactg acgcatacgc tgaatacgct ggcgcatatc agtcagctct
     3841 tgttcccaaa tcgcacgtaa tgcatcgctg ctcaggatgg tggcaacaac agaagcgccg
     3901 tgtgctggag ggttagagta gttagcgcga atcgccgctt tcatttggct gaatgcgcga
     3961 tcaacgattt cactgtcggc agcaaccaga gtacaagcgc caacacgctc gttgtacagg
     4021 ccaaagtttt tagagtagga actggcaaca atcagctctt tatgcatagc cgcgaaagcg
     4081 cgcagtcctt cagcatcttc ttccagacca cgggcaaaac cctggtaagc gaagtcaaac
     4141 agcggtaacc agcctttctc aacggagagt tgtgccagtg tctgccattg ttccagcgta
     4201 gggtcgatac cggttgggtt atggcagcag ccatggaaca gcactacgtc gccagcctga
     4261 gcttcgttca gactgttaat cagtgcatcg aagtcaaggg tgtgattttc cgcatcataa
     4321 taagcgtatt cacgaacttc cagacctgca gagttaaaga cgctcttatg gttcggccag
     4381 cttgggttgc tcacccacac acgcttaacg ctggtatttt ttgccaggaa gtcggcagcc
     4441 acgcgtagtg cgccagtgcc acccggagtc tgtgccgtgc gagcacgttt gtcattgatc
     4501 agggcgctac ctttaccaaa cagcagttcc tgagtgcagc gaccaaattc aaggatgccg
     4561 tcaatgccga ggtaattttt ggtggtttca ttttcgagca gatactgttc agcctttttc
     4621 acgctggtca gtaccggggt tttgcccgtc tcatctttat agacaccaat cccgaggtta
     4681 attttgccgg gacgttcatc ggcacgaaac agatcggcca ggcccagaat cgggtcggca
     4741 ggagcggcgg taatgttctc aaacatgacg aggttccatt atggttacag aagggaagtc
     4801 cgctatcagg gtaacgggag atttacaaaa ttccaactat tactgatgaa aacgcaggct
     4861 gtttttgcaa gacgtgagat tgctctggaa ggcataaaaa aaacaggacc aacgtcctgt
     4921 tttttcggca tttaacaaag aggtgtgcta ttagaactgg taaacgatac ccacagcaac
     4981 ggtgtcgtct gaacctacgc ccagtttgtt gtcagaatcg atctggttga tgatgtagtc
     5041 aacataggtg gacatgtttt tgttgaagta gtaggttgcg cccacttcaa agtagttcac
     5101 cagatcaaca tcaccgatac cttctacgtc tttcgcttta gatttggtgt aagcgatgga
     5161 cggacgcaga ccgaaatcga actggtattg cgcaactaac agaacgtctt gcgttttgtt
     5221 ggcgaagccg ctggtgtttg taaatttatt agtgatcggc gtagcgttac gggtttcacc
     5281 gtagttcgct gccaggtaga tgttgttcgc gtcgtacttc agaccagtag cccactgttc
     5341 agctttttta ccgttgccaa gaggttgagc ttcttgcagg ttggtacggt cagctgcacc
     5401 ataagcacca acgataccaa agccttcgta ttcgtagctg atagaaccgc caacaccgtc
     5461 gccgttagaa cggcgtgcag tgtcacgctc gtttttaccc aggtactgaa cagcgaagtt
     5521 caggccatca accagaccaa agaagttgga gttacgatag gtagcaacgc cgccaacacg
     5581 accaacgaag aagtcatcgc tgtatgcagt atcaccacca aattctggca gcatatcggt
     5641 gtaacccagt gcatcataaa ccacaccgta gttacggccg taatcgaaag aaccaacgtc
     5701 agcgtattta agacccgcga atgccagacg cgttttgtta ccagtttgag cgtcagcgcc
     5761 ttcagagttg ttaccctgga agttatattc ccactgacca taaccggtca gatcggaatt
     5821 gatttgagtt tcccctttaa aaccaagacg ggcataggtc atgtcgccat tgccaccgta
     5881 actgttttca ccgttaccct tggaaaaata atgcagacca acagttttac cgtacagatc
     5941 tactttgttg ccatctttgt tatagatttc tgcagcgttt gcagtacctg ctactaacag
     6001 agcagggacg atcactgcca gaatattgcg cttcatcatt atttattacc ctcatggttt
     6061 ttttatgaca cctgccactg ccgtcaataa gttctgtcaa taaaaattta cggaactatt
     6121 gatgagagtt tggtgtcttt atgtgtctgc aggcatcttt ccattcaaac taacgtttcg
     6181 ctaccgtgaa agtgctacaa agataaagat ttagtttcaa aaagaaaaaa tatgtaacca
     6241 aaagtaaaaa ttaaggagct ttgtgaacac cgtcatattt ccataggaac gtgatgatat
     6301 ttacagcaat ttttatctat ttatatgatt tacttatatt taaattaact aaacggaaat
     6361 tttgtttctg atggaaactt tatcgacctg gcacaaaatc ctctttcaga catccagaat
     6421 gccaaaaaat agtatgaaaa ttgtgctatc agctaagaaa aaactaatcc gcaataagag
     6481 tgcgggttat tttttgtgcg taaatgttgc aatgtaatat tccgtaacag gatgatcgtt
     6541 atggcggggg tgcaaataga ttgcttgact taacaaacag ataacttgac agaaaggata
     6601 aaaaagagcc agcaggcgct ggctctttga ggaagaaaat cagaagctgg cgttgcgcgg
     6661 agtacgtggg aacggaatca catcacgcac gttttgcacg ccagttacgt aagcaatcag
     6721 gcgttcaaaa ccaagaccga aacctgaatg cggaacagta ccgtagcgac gcagatcgcg
     6781 ataccaccag taatcttctt tattcaggcc catttccagc atacgctcgt ccagaacgtc
     6841 cagacgttct tcacgctggg agccaccaat gatctcaccg atgcccggag ccagaacgtc
     6901 catagccgca acggttttac cgtcttcgtt gaggcgcata tagaacgctt taatatcttt
     6961 cggatagttt ttaaccacta ccggtgcttt aaagtgttct tccgccagat aacgctcatg
     7021 ctcagaagag agatcgacgc cccagtaaac cgggttttca aacttcctgc cgcagttttc
     7081 gagaatggtc actgcgtcgg tgtaatccac ctgcgcgaaa tcggcttcaa tgaagcgttc
     7141 cagacgtgaa acggcatctt tatctacgcg ttcagcgaag aatttcatgt cgtctgcgcg
     7201 ttcttcgaga accgctttga agacatattt cagcatggct tcagccagac ccgcgatatc
     7261 gttcaggtta gcaaacgcca cttccggctc cagcatccag aattccgcca ggtgacggct
     7321 ggtgttggag ttttcagcac ggaaagtcgg gccgaaggta taaattttgg ataatgcgca
     7381 agcgtaggtt tcgccgttca actggccaga tacggtcagg aaagactctt taccaaagaa
     7441 gtctttgtcg aaatccactt tgccctgatc gttacgcggc aggttttcca gatccagcgt
     7501 agaaacgcgg aacatttcgc ctgcaccttc ggtatcagat gcggtaatca gtggcgttga
     7561 aacccagaag aatccctgct cgttaaagaa gcgatgcagc gcctgcgcca gcgtatggcg
     7621 aacgcgcgcg acggcaccaa tcaggtttgt gcgcggacgc aggtgagcga cttcacgcag
     7681 atactcaatg ctgtggcgtt ttgccgccat cgggtaagtg tctggatctt caacccaacc
     7741 agcaacttca accttgctgg cctgaatttc aaattgttgc ccctggcccg gcgatgccac
     7801 gactttaccc gtcacaatga ccgagcagcc ggtggtcaga cgcaggacgt cttcattgta
     7861 attgggcaga gaattattga tgacagcctg tacaggatca aagcaggaac cgtcataaac
     7921 ggcgaggaag gagatgccag cttttgaatc tcggcgggta cgtacccatc cgcgtacggt
     7981 gacttcgctg tcaacggcta cgcggccctg gagtacgtcg gctacaggca caacgctcat
     8041 aatattctct ctgttaatag tcggaaaaaa taaacacttg tccacccgta aatggggggg
     8101 atacctatgt tacctggcat ctgcaatcag acaagcagaa ttcgcaaatg cagcgaaaga
     8161 tttcggaatt aaaggtaaaa aagggagccg attagctccc tgatgatatt aactggcttt
     8221 tttaatatgc ggaaggtcga acgctttgcg cagcgcccga acaaacgctt tatcatggca
     8281 gatagttttg ccagggctgt cagaaagttt cgccaccggt ttaccgttac actctaccaa
     8341 cttaatcaca atattcaggg gttttacctg ggggatatcg caggtcaggc gagtcccaat
     8401 accaaaactt aattgcacgc gggaagagaa gtggcggtat agctcaaccg ctttgcgtaa
     8461 atccagattg tcagagaaaa ccagcgtttt actctgtgga tcaattccca gcttttcata
     8521 atgtgcaatg gctttttcac cccattcaac cgggtcgcca gagtcatgac gcaggccctg
     8581 ataccgacta gcgaactcga caccgaaatc acgcaggaaa gcatccatag tgatgcagtc
     8641 ggttaatgca atgccaagtt ggtcgggata ctcttccagc caggcagcaa gtgcagctcg
     8701 ctggctgttg gctagatccg ggctgatttg ctgatgtgcc tggaaccatt cgtgtgcctg
     8761 tgttcccatc ggcgtgaggg aaagccgacg cgccagatcg tagttgctgg tgcccacgaa
     8821 ccaggattcc tgttgcagac gcttaacgat ggtttcttgt acttcgcgag aaaaacggcg
     8881 acgggtgcca aaatccatca gatggaagcg cgacatatca agaccggcgg ttaacgccga
     8941 gaagtcgact aatttgcttt ccagcgtgtc gagggcttgc gcaacgtcgg cctgcggtga
     9001 gcgatagcga tgtaccattt cactgataac cgccagcaaa ggaacttccc agaggatgac
     9061 ttcacgccac ggaccgctta agcgaatatc cagcttgcca ttatcgttgg acacggtgac
     9121 ttgttccggg ttaaagcgga actcgcgtaa ccagttaaga tagtcggcct taaagaaagg
     9181 cagggcagag agccactgat attcatcatc ctgcaggcgc aggtgctgca tcgcctgaat
     9241 ctgttcacga atagcatcgg cataaatacc cagcagatcg tcacctcggc aacgaaactc
     9301 cgccgcgaca tgcacatcgt aatagtgatg aaacacggct tgctgcatat gcaacttata
     9361 agcatctgta tccagcaacg agtgcagaac aggagaagcg aattgtgtca taggtgcgca
     9421 gtagcgtcct cgtacaggag cgtttagtac aataaacatc ttcaggaact gctggagtat
     9481 accttgtttc gcaatttatt gaaccccgat cacaccatat gccacctttc tggtcgatgg
     9541 cattacgcgg tgtatgttat aaaaatgtag caataaaggc gtttgtacct gaaaagatga
     9601 agattctgca tagcgcgatt tacgcaacag gaatagactg aacaccagac tctataaaag
     9661 atgctaaagg ttatttatga ctcaacagcc acaagccaaa taccgtcacg attatcgtgc
     9721 gccggattac cagattactg atattgactt gacctttgac ctcgacgcgc aaaagacggt
     9781 cgttaccgcg gtcagccagg ctgtccgtca tggtgcatca gatgctccgc ttcgtctcaa
     9841 cggcgaagac ctcaaactgg tttctgttca tattaatgat gagccgtgga ccgcctggaa
     9901 agaagaagaa gagggcgcac tggtcatcag taatttgccg gagcgtttta cgcttaagat
     9961 cattaatgaa ataagcctgg cggcgaatac ggcgctggaa gggctttatc agtcaggcga
    10021 tgcgctttgc acccagtgtg aagccgaagg tttccgccat attacgtatt atctcgaccg
    10081 cccggacgtg ctggcgcgtt ttaccaccaa aattattgcc gataaaatca aatatccctt
    10141 cctgctttcc aatggtaacc gcgttgcgca aggcgaactc gaaaacggac gccattgggt
    10201 acagtggcag gacccgttcc cgaaaccgtg ctacctgttt gcgctggtgg caggcgactt
    10261 tgatgtactg cgcgatacct ttaccacgcg ttctggtcgc gaagtagcac tggagctgta
    10321 cgtcgatcgc ggcaaccttg atcgcgcgcc gtgggcgatg acctcgctga aaaactccat
    10381 gaaatgggat gaagaacgct ttggcctgga gtatgacctc gacatctata tgatcgtcgc
    10441 ggtggatttc ttcaatatgg gcgcaatgga gaataagggt ctgaatatct ttaactccaa
    10501 atatgtgctg gcccgcaccg acaccgccac cgacaaagat tacctcgata ttgaacgcgt
    10561 tatcggccat gaatatttcc ataactggac cggtaaccga gtgacctgcc gcgactggtt
    10621 ccagctcagc ctgaaagaag gtttaaccgt cttccgcgat caggaattca gctctgacct
    10681 tggttcccgc gcagttaacc gcatcaataa tgtgcgcacc atgcgcggat tgcagtttgc
    10741 ggaagacgcc agcccgatgg cgcacccaat ccgcccggac atggtcattg agatgaacaa
    10801 cttctacacc ctgaccgttt acgagaaggg cgcggaagtg atccgtatga tccacaccct
    10861 gctgggcgaa gaaaacttcc agaaagggat gcaactctat ttcgagcgtc atgatggtag
    10921 cgcagcgacc tgtgacgact ttgtgcaggc gatggaagat gcgtcgaatg ttgatctctc
    10981 ccatttccgc cgttggtaca gccagtccgg tacgccggtt gtgaccgtca aagacgacta
    11041 caatccggaa actgagcagt acaccctgac catcagccag cgcacgccag ccacgccgga
    11101 tcaggcagaa aaacagccgc tgcatattcc gtttgccatc gaattgtatg acaacgaagg
    11161 taaagtgatc ccgttgcaga aaggcggtca tccggtgaat tccgtgctga acgtcaccca
    11221 ggcggaacag acctttgttt ttgataatgt ctacttccag ccggtgcctg cgctgctgtg
    11281 cgaattctct gcgccagtga aactggaata taagtggagc gatcagcaac tgaccttcct
    11341 gatgcgtcat gcgcgtaatg gtttctcccg ttgggatgcg gcgcaaagtt tgctggcaac
    11401 ctacatcaag ctgaacgtcg cccgtcatca gcaagggcag ccgctgtctc tgccggtaca
    11461 tgtggctgac gctttccgcg cggtgctgct cgatgagaag attgatccgg cgctggcggc
    11521 agaaatcctg acgctgcctt ctgtcaatga aatggctgaa ctgttcgata tcatagaccc
    11581 gattgctatt gccgaagtac gcgaagcact cactcgtact ctggcgactg aactggcgga
    11641 tgagctgctg gctatttaca acgcgaatta ccagagcgag taccgtgttg agcatgaaga
    11701 tattgcaaaa cgcactctgc gtaatgcctg cctgcgcttc cttgcttttg gtgaaacgca
    11761 tctggctgac gtgctggtga gcaagcaata ccatgaagca aacaatatga ccgatgcgct
    11821 ggcagcgctt tctgcggcgg ttgctgcaca gctgccttgc cgtgacgcgc tgatgcagga
    11881 gtacgacgat aagtggcatc aggacggtct ggtgatggat aaatggttta tcctgcaagc
    11941 caccagcccg gcggcgaatg tgctggagac ggtgcgtggt ctgttgcagc atcgctcatt
    12001 taccatgagc aacccgaacc gtattcgttc gttgattggt gcgtttgcgg gcagcaaccc
    12061 ggcagcgttc catgccgaag atggcagcgg ttaccagttc ctggtggaaa tgcttaccga
    12121 cctcaacagc cgtaacccgc aggtagcttc acgtctgatt gaaccgctga ttcgcctgaa
    12181 acgttatgat gccaaacgtc aggagaaaat gcgcgcggcg ctggaacagt tgaaagggct
    12241 ggaaaatctc tctggcgatc tttacgagaa gataactaaa gcactggctt gatacttttg
    12301 agccaaaatg cctgatgcga cgcttgacgc gtcttatcag gcctacaact ccgttgaaat
    12361 tgctggtttt gtaggctgga taaggcgttc acgccgcatc cggcagccgt gcccaactgc
    12421 ctgatgcgat gcttgacgtg tcttatcagg cctacaagtc cgttgaaaat gctggttttg
    12481 tgcgccacat ccggcatatt aaccatgacg ccgaatcagt tgttcagaat gcccacgccg
    12541 cattacccgt tgcaatactt ccgcttccag ctccgctaat cgcaccgacc ctaatcgacg
    12601 cggacggggg atatccactg tcaaatccag actaattttt ccctcttcaa ttaacaacac
    12661 ccggtcagcc attgctaccg cttcgctcac atcatgcgtc accaacagta cggtaaagcc
    12721 gtgctgctgc caaagtgaca caatcaaatc ctgcatctcg agtcgcgtta aggcgtccag
    12781 tgccccgagc ggttcatcaa gcaacaataa acccggtcga tgaatcaacg ctcttgccag
    12841 cgccacacgc tgtttctgcc cgccagaaag tgcggcaggc cattccccgg cgcgattctc
    12901 cagtcctgcc gcagccaacg cccgacgcgc ggcatcgcgc cactgacctt taaggcctaa
    12961 cccaacgtta tcaatcaccg atttccacgg cagcagacgc gcatcctgaa acatcattcg
    13021 cgtatcatcc tgaatttcag ccagcggtgt ggtgcccgct aacacatcgc ctgcggttgg
    13081 cgtttccaga ccggccagca ggtgcagcag ggtactttta ccaccaccgc tgcggcccac
    13141 caccgccaca aactgacctg ccggaatatg taaatccagt tggttcagga cgatattttc
    13201 cgcgtaatgt ttgcttactg cattgagcaa caatggtgtg ccctggttca gacgagcagt
    13261 attcatatcg tggcctcctt caaatgataa gccgggttcc agcgcaacca gagg
//
LOCUS       DAJTRC010000096        13108 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.096, whole genome shotgun sequence.
ACCESSION   DAJTRC010000096 DAJTRC010000000
VERSION     DAJTRC010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13108
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.096"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(1..858)
                     /gene="kdgT"
                     /locus_tag="OO357_003152"
                     /pseudo
     CDS             complement(1..858)
                     /gene="kdgT"
                     /locus_tag="OO357_003152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709713.2"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-keto-3-deoxygluconate transporter"
     gene            complement(1118..1738)
                     /gene="sodA"
                     /locus_tag="OO357_003153"
     CDS             complement(1118..1738)
                     /gene="sodA"
                     /locus_tag="OO357_003153"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010345169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase [Mn]"
                     /protein_id="HCR5888003.1"
                     /translation="MSYTLPSLPYAYDALEPHFDKQTMEIHHTKHHQTYVNNANAALE
                     SLPEFANLPVEELITKLDQLPADKKTVLRNNAGGHANHSLFWKGLKKGTTLQGDLKAA
                     IERDFGSVDNFKAEFEKAAASRFGSGWAWLVLKGDKLAVVSTANQDSPLMGEAISGAS
                     GFPILGLDVWEHAYYLKFQNRRPDYIKEFWNVVNWDEAAARFAAKK"
     gene            2023..3057
                     /gene="rhaT"
                     /locus_tag="OO357_003154"
                     /pseudo
     CDS             2023..3057
                     /gene="rhaT"
                     /locus_tag="OO357_003154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418343.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose/proton symporter RhaT"
     gene            complement(3054..3920)
                     /gene="rhaR"
                     /locus_tag="OO357_003155"
     CDS             complement(3054..3920)
                     /gene="rhaR"
                     /locus_tag="OO357_003155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaR"
                     /protein_id="HCR5888004.1"
                     /translation="MAEVATVAHQLKLLKDDFFASDQQAVAVADRYPQDVFAEHTHDF
                     CELVIVWRGNGLHVLNDRPYRITRGDLFYIHADDKHSYASVNDLVLQNIIYCPERLKL
                     NLDWQGAIPGFSASAGQPHWRLGSVGMAQARQVIGQLEHESSQHVPFANEMAELLFGQ
                     LVMLLNRHRYTSDSLPPTSSETLLDKLITRLAASLKSPFALDKFCDEASCSERVLRQQ
                     FRQQTGMTINQYLRQVRVCHAQYLLQHSRLLISDISTECGFEDSNYFSVVFTRETGMT
                     PSQWRHLNSQKD"
     gene            complement(3976..4812)
                     /gene="rhaS"
                     /locus_tag="OO357_003156"
     CDS             complement(3976..4812)
                     /gene="rhaS"
                     /locus_tag="OO357_003156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional activator RhaS"
                     /protein_id="HCR5888005.1"
                     /translation="MTVLHSVDFFPSCNASVAIEPRLPQADFPEHHHDFHEIVIVEHG
                     TGIHVFNGQPYTITGGTVCFVRDHDRHLYEHTDNLCLTNVLYRSPDRFQFLAGLNQLL
                     PQELDGQYPSHWRVNHSVLQQVRQLVAQMEQQEGENDLPSTASREILFMQLLLLLRKS
                     SLQENLENSASRLNLLLAWLEDHFADEVNWDAVADQFSLSLRTLHRQLKQQTGLTPQR
                     YLNRLRLMKARHLLRHSEASVTDIAYRCGFSDSNHFSTLFRREFNWSPRDIRQGRDGF
                     LQ"
     gene            5100..6569
                     /gene="rhaB"
                     /locus_tag="OO357_003157"
     CDS             5100..6569
                     /gene="rhaB"
                     /locus_tag="OO357_003157"
                     /EC_number="2.7.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulokinase"
                     /protein_id="HCR5888006.1"
                     /translation="MTFRNCVAVDLGASSGRVMLARYERECRSLTLREIHRFNNGLHS
                     QNGYVTWDVDSLESAIRLGLNKVCEEGIRIDSIGIDTWGVDFVLLDQQGQRVGLPVAY
                     RDSRTNGLMPQAQQQLGKRDIYQRSGIQFLPFNTLYQLRALTEQQPELIPHIAHALLM
                     PDYFSYRLTGKMNWEYTNATTTQLVNINSDDWDESLLAWSGANKAWFGRPTHPGNVIG
                     HWICPQGNEIPVVAVASHDTASAVIASPLNGSRAAYLSSGTWSLMGFESQTPFTNDTA
                     LAANITNEGGAEGRYRVLKNIMGLWLLQRVLQERQINDLPALIAATQALPACRFIINP
                     NDDRFINPDEMCSEIQAACRETAQPIPGSDAELARCIFDSLALLYADVLHELAQLRGE
                     DFSQLHIVGGGCQNTLLNQLCADACGIRVIAGPVEASTLGNIGIELMTLDELNNVDDF
                     RQVVSTTANLTTFTPNPDSEIAHYVAQIHSTRQTKELCA"
     gene            6566..7825
                     /gene="rhaA"
                     /locus_tag="OO357_003158"
     CDS             6566..7825
                     /gene="rhaA"
                     /locus_tag="OO357_003158"
                     /EC_number="5.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose isomerase"
                     /protein_id="HCR5888007.1"
                     /translation="MTTQLEQAWELAKQRFAAVGIDVEEALRQLDRLPVSMHCWQGDD
                     VSGFENPEGSLTGGIQATGNYPGKARNASELRADLEQAMRLIPGPKRLNLHAIYLESD
                     TPVSRDQIKPEHFKNWVEWAKANQLGLDFNPSCFSHPLSADGFTLSHPDDSIRQFWID
                     HCKASRRVSAYFGEQLGTPSVMNIWIPDGMKDITVDRLAPRQRLLAALDEVISEKLNP
                     AHHIDAVESKLFGIGAESYTVGSNEFYMGYATSRQTALCLDAGHFHPTEVISDKISAA
                     MLYVPQLLLHVSRPVRWDSDHVVLLDDETQAIASEIVRHDLFDRVHIGLDFFDASINR
                     IAAWVIGTRNMKKALLRALLEPTAELRKLEAAGDYTARLALLEEQKSLPRQAVWEMYC
                     QHHDTPAGSEWLESVRAYEKAILSQRG"
     gene            8067..8891
                     /gene="rhaD"
                     /locus_tag="OO357_003159"
                     /pseudo
     CDS             8067..8891
                     /gene="rhaD"
                     /locus_tag="OO357_003159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312856.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnulose-1-phosphate aldolase"
     gene            8901..9215
                     /gene="rhaM"
                     /locus_tag="OO357_003160"
     CDS             8901..9215
                     /gene="rhaM"
                     /locus_tag="OO357_003160"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HCR5888008.1"
                     /translation="MIRKAFVMQVNPDAHEEYQRRHNPIWPELEAVLKSHGVHNYAIY
                     LDKARNLLFAMVEIESEERWNAVASTDVCQRWWKYMTDVMPANPDNSPVSSELQEVFY
                     LP"
     gene            9516..9962
                     /locus_tag="OO357_003161"
     CDS             9516..9962
                     /locus_tag="OO357_003161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709704.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HCR5888009.1"
                     /translation="MAALTASCIDLNIQGNGAYSVLKQLATIALQNGFITDSHQFLQT
                     LLLREKMHSTGFGSGVAVPHGKSACVKQPFVLFARKAQAIDWKASDGEDVNCWICLGV
                     PQSGEEDQVKIIGTLCRKIIHQEFIHQLQQGDTDQVLALLNQTLSS"
     gene            9973..11424
                     /locus_tag="OO357_003162"
     CDS             9973..11424
                     /locus_tag="OO357_003162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose-like transporter subunit IIBC"
                     /protein_id="HCR5888010.1"
                     /translation="MESSLRIVAITNCPAGIAHTYMVAEALEQKARSLGHTIKVETQG
                     SSGVENRLSSEEIAAADYVILATGRGLSGDDRARFAGKKVYEIAISLALKNIDQIFSE
                     LPTNSQLFAADSGVKLGKQEVQSGSVMSHLMAGVSAALPFVIGGGILVALANMLVQFG
                     LPYTDMSKGAPSFTWVVESIGYLGFTFMIPIMGAYIASSIADKPAFAPAFLVCYLAND
                     KALLGTQSGAGFLGAVVLGLAIGYFVFWFRKVRLGKALQPLLGSMLIPFVTLLVFGVL
                     TYYVIGPVMSDLMGGLLHFLNTIPPSMKFAAAFLVGAMLAFDMGGPINKTAWFFCFSL
                     LEKHIYDWYAIVGVVALMPPVAAGLATFIAPKLFTRQEKEAASSAIVVGATVATEPAI
                     PYALAAPLPMITANTLAGGITGVLVIAFGIKRLAPGLGIFDPLIGLMSPVGSFYLVLA
                     IGLALNISFIIVLKGLWLRRKAKAAQQELVHEH"
     gene            11414..12484
                     /locus_tag="OO357_003163"
     CDS             11414..12484
                     /locus_tag="OO357_003163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HCR5888011.1"
                     /translation="MNIELLQQLCEASAVSGDEQEVRDILINTLEPCVNEITFDGLGS
                     FVARKGNKGPKVAVVGHMDEVGFMVTHIDESGFLRFTTIGGWWNQSMLNHRVTIRTHK
                     GVKIPGVIGFVAPHALTEKQRQQPLSFDEMFIDIGANSRDEVEKRGVAMGDFISPEAN
                     FACWGEDKVVGKALDNRIGCAMMAELLQTVNNPEITLYGVGSVEEEVGLRGAQTSAEH
                     IKPDVVIVLDTAVAGDVPGIDNIKYPLKLGQGPGLMLFDKRYFPNQKLVAALKNCAAH
                     NDLPLQFSTMKTGATDGGRYNVMGGGRPVVALCLPTRYLHANSGMISKADYDALLTLI
                     RGFLTTLTAEKVNAFSQFRQVD"
     gene            12484..>13108
                     /locus_tag="OO357_003164"
     CDS             12484..>13108
                     /locus_tag="OO357_003164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HCR5888012.1"
                     /translation="MLNERQLKIVDLLEQQPRTPGELAQQTGVSGRTILRDIDYLNFT
                     LNGKARIFASGSAGYQLEIFERRSFFQLLQKHDNDDRLLALLLLNTFTPRAQLASALN
                     LPETWVAERLPQLKQRYERTCCLASRPGLGHFIDETEEKLVILLANLLRKDPFLIPLA
                     GITRDNLQHLSTACDNQHRWPLIQGDYLSSLILAIYALRNQLTDEWPQ"
BASE COUNT         3119 a         3427 c         3459 g         3103 t
ORIGIN      
        1 ctgccggagc catcggttta aacgcaggca ccatttctgc aatcagcaca ggtgtcgcta
       61 ccgcggcccc tgcggaactg gaagcggcaa ttccggcagt gccatcgcca ccgccaatca
      121 atttatcggc gataatcaac ggaataccgg tcacgataat tactgccaca cccaacagga
      181 tccccagcaa accagtctgg gcaatcacag tcaaatcaat ggtattgccc agcgcgaagg
      241 caaagaatgg aatcagcgtt tgcaccgctt tgctgaaaaa ttctcgtaat tcagggtcaa
      301 gattccccag ggcaaagccc accaggaacg gtaatactgc gccgacgaaa acatgcggtt
      361 caaacgaggc aatcccggca gtgcccagaa taatcatcgt catgagcggc ccggactcca
      421 acgacatcaa cacaaatgcc cagcttcttc tttcgtaccg tactgctgca tgatggaagc
      481 gtaaagtccg ccgttggtca tatccatcgc cgccaccagc gccagcgttg aaagtccggc
      541 aaagaatcca acttcaacac catgttccgg aataatgcgc gaggcaattg ccgcaaccac
      601 ccacgcgacg gcaattttag ttaccaccag cgtaccggat ttacgcagta ccgttcccgt
      661 cgcgcttaat tttattgacg cccccatgca aaaaaaccac accgccagaa tgggcaccgt
      721 accggtaatc atcccgttgg tgaatgatcc aaaatatttc cccgcccccg gcgagaaggt
      781 gtggcacagt gcgccaagga atagcgggac gagcatcatc ccccccggga ttttctcaat
      841 cgagcgtttt atctgcattt ccatcacctg tcggcatatt tttaagggct gatgcgtaat
      901 tcctcaatgg agcatcagtg ttgatggaaa cgaagataga cattcattta atcaataaaa
      961 gtgatcaaaa tcacatcaat tgaaacgctg ttttattttt ataggaccat tgtttttatt
     1021 ttggaattga tcacaaaaaa accaccgccg ttggcgatgg ttcatttttt aagctgatat
     1081 gcggcctata cgcctcattg cagcaggcgg caaatgatta ttttttcgcc gcaaaacgtg
     1141 ccgctgcttc gtcccagttc accacgttcc agaactcttt gatgtagtcc ggacggcggt
     1201 tctggaattt caggtagtaa gcatgttccc acacatccag gcccagaatc gggaagccgg
     1261 aagcgccaga aatagcttca cccatcagcg gagaatcctg gttagcagta gaaaccaccg
     1321 ccagtttatc gcctttcagc accagccatg cccagccgga accaaagcgg gaagctgccg
     1381 ctttttcaaa ttctgctttg aagttatcaa cggagccgaa gtcacgttcg atagccgctt
     1441 tcaggtcacc ctgcagggtg gtgccttttt tcagaccttt ccagaacagg ctgtggttag
     1501 cgtgaccgcc agcgttgttg cgcagtacgg ttttcttgtc tgctggcagc tggtccagtt
     1561 tggtgatcag ctcttcaacc ggcaggttgg caaattctgg caggctttcc agcgccgcgt
     1621 tggcgttgtt tacgtaggtc tgatggtgtt tggtgtggtg gatttccatg gtctgcttat
     1681 cgaagtgcgg ttccagggca tcgtaagcat acggcaggga tggcagggta tagctcatat
     1741 tcatctccag tattgtcggg cggccgattg ttaatgccgc gtaagcagtt ggttcattat
     1801 agttaattaa atgatattga aaatgattat caatgccgta cttttcgtaa gggtatggtt
     1861 ttgcaggaaa atgcccgaga tgtgaagcaa atcacccact taatgccgtg attgccagta
     1921 aatcgacaac ggcggcaaca ggcgaaaggt taatcgacag cacgattttt acactcatct
     1981 cgtcggagat gtgaagcgac gaaaaatgat gaggataaga agatgagtaa cgcgattacg
     2041 atggggatat tttggcattt gatcggcgcg gccagtgcag cctgttttta cgctccgttc
     2101 aaaaaagtaa aaaaatggtc atgggaaacc atgtggtcag tcggtgggat tgtttcgtgg
     2161 attattctgc cgtgggccat cagcgccctg ttactaccgg atttctgggc gtattacagc
     2221 tcgtttagtc tctctacgct gctgcctgtt tttctgttcg gcgctatgtg ggggatcggt
     2281 aatatcaact atggcctgac catgcgttat ctcggcatgt cgatgggaat tggcatcgcc
     2341 attggcatta cgttgattgt cggtacgctg atgacgccaa ttatcaacgg caatttcgat
     2401 gtgctgatta acaccgaagg cggacgcatg acgttactcg gcgttctggt ggcgctgatt
     2461 ggcgtaggga ttgtaactcg agccgggcag ttgaaagagc gcaagatggg cattaaagcc
     2521 gaagagttca atctgaaaaa agggctggtg ctggcggtga tgtgcggcat tttctctgcc
     2581 gggatgtcct ttgcgatgaa cgccgcaaaa ccgatgcatg aagccgctgc cgcacttggc
     2641 gtcgatccac tgtatgttgc tctgccaagc tatgttgtca tcatgggcgg cggcgcgatc
     2701 attaacctcg gtttctgttt tattcgtctg gcaaaagtga aggatttgtc gctaaaagcc
     2761 gacttctcgc tggcaaaacc gctgatcatt cacaatgtgt tactctcggc actgggcggg
     2821 ttgatgtggt atctgcaatt ctttttctat gcctagggcc acgcccgcat tccggcgcag
     2881 tatgactaca tcagttggat gctgcatatg agtttctatg tattgtgcgg cggtatcgtc
     2941 gggctggtgc tgaaagagtg gaacaataca ggacgccgtc cggtaacggt gttgagcctc
     3001 ggttgtgtgg tgattattgt cgccgctaac atcgtcggca tcggcatggc gaattaatct
     3061 ttctgcgaat tgagatgacg ccactggctg ggcgtcatcc cggtttcccg ggtaaacacc
     3121 accgaaaaat agttactatc ttcaaagcca cattcggtcg aaatatcact gattaacagg
     3181 cggctatgct ggagaagata ttgcgcatga cacactctga cctgccgcag atattgattg
     3241 atggtcattc cagtctgctg gcgaaattgc tgacgcaaaa cgcgctcact gcacgatgcc
     3301 tcatcacaaa atttatccag cgcaaaggga cttttcaggc tagccgccag ccgggtaatc
     3361 agcttatcca gcaacgtttc gctggatgtt ggcggcaacg aatcactggt gtaacgatgg
     3421 cgattcagca acatcaccaa ctgcccgaac agcaactcag ccatttcgtt agcaaacggc
     3481 acatgctgac tactttcatg ctcaagctga ccgataacct gccgcgcctg cgccatcccc
     3541 acgctaccta agcgccagtg tggttgccct gcgctggcgc taaatcccgg aatcgctccc
     3601 tgccagtcaa gattcagctt cagccgctcc gggcaataaa taatattctg caaaaccaga
     3661 tcgttaacgg aagcgtagga gtgtttatcg tcagcatgaa tataaaagag atcgccacgg
     3721 gtaatgcgat aaggacgatc gttgagtaca tgcagaccat taccgcgcca gacaatcacc
     3781 agctcacaaa aatcatgtgt atgttcggca aagacatctt gcggataacg gtcagccaca
     3841 gcgactgcct gctggtcgct ggcaaaaaaa tcatctttga gaagttttaa ctgatgcgcc
     3901 accgtggcta cctcggccag agaacgaagt tgattattcg caatatggcg tacaaatacg
     3961 ttgagaagat tcgcgttatt gcagaaagcc atcccgtccc tggcgaatat cacgcggtga
     4021 ccagttaaac tctcggcgaa aaagcgtcga aaagtggtta ctgtcgctga atccacagcg
     4081 ataggcgatg tcagtgacgc tggcctcgct gtggcgtagt agatgtcggg ctttcatcag
     4141 tcgcaggcgg ttcaggtatc gctgaggcgt cagtcccgtt tgctgcttaa gctgccgatg
     4201 tagcgtacgc agtgaaagag aaaattgatc cgccacggca tcccaattca cctcatcggc
     4261 aaaatggtcc tccagccagg ccagaagcaa gttgagacgt gatgcgctgt tttccaggtt
     4321 ctcctgcaaa ctgcttttac gcagcaagag cagtaattgc ataaacaaga tctcgcgact
     4381 ggcggtcgag ggtaaatcat tttccccttc ctgctgttcc atctgtgcaa ccagctgtcg
     4441 cacctgctgc aatacgctgt ggttaacgcg ccagtgagac ggatactgcc catccagctc
     4501 ttgtggcagc aactgattca gcccggcgag aaactgaaat cgatccggcg agcgatacag
     4561 cacattggtc agacacagat tatcggtatg ttcatacaga tgccgatcat gatcgcgtac
     4621 gaaacagacc gtgccaccgg tgatggtata gggctgccca ttaaacacat gaatacccgt
     4681 gccatgttcg acaatcacaa tttcatgaaa atcatgatga tgttcaggaa aatccgcctg
     4741 cgggagccgg ggttctatcg ccacggacgc gttacaagac ggaaaaaaat ccacactatg
     4801 taatacggtc atactggcct cctgatgtcg tcaacacggc gaaatagtaa tcacgaggtc
     4861 aggttcttac cttaaatttt cgacggaaaa ccacgtaaaa aacgtcgatt tttcaagata
     4921 cagcgtgaat tttcaggaaa tgcggtgagc atcacatcac cacaattcag caaattgtga
     4981 acatcatcac gttcatcttt ccctggttgc caatggccca ttttcctgtc agtaacgaga
     5041 aggtcgcgaa ttcaggcgct ttttagactg gtcgtaatga aattcagcag gatcacatta
     5101 tgacctttcg caattgtgtc gccgtcgatc tcggcgcatc cagtgggcgc gtgatgctgg
     5161 cgcgttacga gcgtgaatgc cgcagcctga cgctgcgcga aatccatcgt tttaacaatg
     5221 ggctgcatag ccaaaacggt tatgtcacct gggatgtgga tagcctggaa agtgccattc
     5281 gccttggatt aaacaaggtg tgcgaggaag ggattcgtat cgatagcatt gggattgata
     5341 cctggggcgt ggactttgtg ctgctcgacc aacagggtca gcgtgtgggc ctgcccgttg
     5401 cttatcgcga tagccgcacc aatggcctga tgccgcaggc acaacagcaa ctcggcaaac
     5461 gcgatattta tcagcgtagc ggcatccagt ttctaccctt caatacgctt tatcagttgc
     5521 gtgcgctgac ggagcaacaa cctgaactta ttccacacat tgctcacgct ctgctgatgc
     5581 cggattactt cagttatcgc ctgaccggca agatgaactg ggaatatacc aatgccacca
     5641 ccacacaact ggtcaatatc aatagcgacg actgggacga gtcgctactg gcgtggagcg
     5701 gggccaacaa agcctggttt ggtcgcccga cgcatccggg taatgtcatt gggcactgga
     5761 tttgcccgca gggtaatgag attccggtgg tcgccgttgc cagccatgat accgccagcg
     5821 cggttatcgc ctcgccgtta aacggttcac gcgccgctta tctctcttct ggcacctggt
     5881 cattgatggg cttcgaaagc cagacgccat ttaccaatga cacggcgctg gcagccaaca
     5941 tcaccaatga aggcggggcg gaaggtcgct atcgggtgct gaaaaatatt atgggcttat
     6001 ggctgcttca gcgagtgctt caggaacggc aaatcaacga tctcccggcg cttatcgccg
     6061 cgacacaggc acttccggcc tgccgcttca tcatcaatcc caatgacgat cgctttatta
     6121 atcctgacga gatgtgcagc gaaattcagg ctgcgtgtcg ggaaacggcg caaccgatcc
     6181 cgggaagtga tgctgaactg gcgcgctgta ttttcgacag tctggcgctg ctgtatgccg
     6241 atgtgttgca tgagctggcg cagctgcgcg gtgaagattt ctcgcaactg catattgtcg
     6301 gcggcggctg ccagaacacg ctgctcaacc agctatgcgc cgatgcctgc ggtattcggg
     6361 tgatcgccgg gcctgttgaa gcctcaacgc tcggcaatat cggcatcgag ttaatgacgc
     6421 tggatgaact caacaatgtg gatgatttcc gtcaggtcgt cagcaccacc gcgaatctga
     6481 ccacctttac ccctaatcct gacagtgaaa ttgcccacta tgtggcgcag attcactcta
     6541 cacgacagac aaaggagctt tgcgcatgac cactcaactg gaacaggcct gggagctggc
     6601 gaaacagcgt ttcgcggcgg tggggattga tgtcgaggag gcgctgcgcc aacttgatcg
     6661 tttacccgtt tcaatgcact gctggcaggg cgatgatgtt tccggttttg aaaacccgga
     6721 aggttcgctg accgggggga ttcaggccac aggcaattat ccgggcaaag cgcgtaatgc
     6781 cagtgagcta cgtgccgatc tggaacaggc tatgcggctg attccggggc cgaaacggct
     6841 taatttacat gccatctatc tggaatcaga tacgccagtc tcgcgcgacc agatcaaacc
     6901 agagcacttc aaaaactggg ttgaatgggc gaaagccaat cagctcggtc tggattttaa
     6961 cccctcctgc ttttcgcatc cgctaagcgc cgatggcttt acgctctcgc atcccgacga
     7021 cagcattcgc cagttctgga ttgatcactg caaagccagc cgtcgcgttt cggcctattt
     7081 tggcgagcaa ctcggcacac catcggtgat gaacatctgg atcccggatg gtatgaaaga
     7141 tatcaccgtt gaccgtctcg ccccgcgtca gcgtctgctg gcagcactgg atgaggtgat
     7201 cagcgagaag ctaaaccctg cgcaccatat cgacgccgtt gagagcaaat tgtttggcat
     7261 tggcgcagag agctacacgg ttggctccaa tgagttttac atggggtatg ccaccagccg
     7321 ccagactgcg ctgtgcctgg acgccgggca cttccacccg actgaagtga tttccgacaa
     7381 gatttccgcc gccatgctgt atgtaccgca gttgctgctg cacgtcagcc gtccggttcg
     7441 ctgggacagc gatcacgtag tgctgctgga tgatgaaacc caggcaattg ccagtgagat
     7501 tgtgcgtcac gatctgtttg accgggtgca tatcggcctt gacttcttcg atgcctctat
     7561 caaccgcatt gccgcgtggg tcattggtac acgcaatatg aaaaaagccc tgctgcgtgc
     7621 gttgctggaa cctaccgctg agctgcgcaa gctggaagcg gcgggcgatt acactgcgcg
     7681 tctggcactg ctggaagagc agaaatcgtt gccgcggcag gcggtctggg aaatgtattg
     7741 ccaacatcac gatacgccag caggtagcga atggctggag agcgtgcggg cttatgagaa
     7801 agcgattttg agccagcgtg ggtaaacact gccggatgcg gcacgagcgc cttatccggc
     7861 ctacgggtcg aatgtccatg cgggctgtca ccctcacccc ggccctctcc ctgaaagggc
     7921 gagggagaaa aacatagcct cgtgcggcaa cggagctgaa caccgctccg gacagtcccc
     7981 tcgcccctac ggggagaggg ttagggtgag gggaacaagc cgcaccatgt ctaaacaagg
     8041 caacctaatt tacaggaaca cagaacatgc aaaacattac ccagtcctgg tttgtccagg
     8101 gaatgatcaa agccaccacc gatgcctagc tgaaaggctg ggacgagcgc aacggcggca
     8161 acctgacgct gcgcctggat gacgccgata tcgcgcttta tcacgacaac ttccacccac
     8221 aaccgcgcta tattccgctc agccaaccca tgcctttact ggcaaatacg ccgtttattg
     8281 tcaccggctc cggcaaattc ttccgtaacg tccagctcga tcctgcagct aacttaggcg
     8341 tcgtaaaagt cgacagcgat ggcgcgggct accatattct ctgggggtta accaacgaag
     8401 ccgttccaac ttctgaactt ccggctcact ttctctccca ctgcgagcgc attaaagcca
     8461 ccaacggtaa agatcgggtg atcatgcact gccacgccac caacctgatc gccctcacct
     8521 atgtactgga aaacgacacc gcggtcttta ctcgccaact gtgggaaggc agcaccgaat
     8581 gtctggtagt attcccggat ggcgttggca ttctgccgtg gatggtgccc ggcacggacg
     8641 aaatcggcca ggcgaccgca caagagatgc aaaaacattc gctggtgttg tggcccttcc
     8701 acggtgtgtt cggttccgga ccgacgctgg atgaagcctt tggtttaatc gacaccgcag
     8761 aaaaatcagc acaagtatta gtgaaggttt attcgatggg cggcatgaaa cagaccatca
     8821 gccgtgaaga gttgatagcg ctcggcaagc gcttcggcgt tacaccacta gccagtgcgc
     8881 tggcgctgta aggagcaaac atgatccgca aagcctttgt catgcaggta aaccccgacg
     8941 cccacgaaga gtatcagcgt cggcataatc ccatctggcc ggaactggaa gcagtgttga
     9001 aatctcacgg tgtgcataac tacgccattt acctcgacaa agcgcgtaat ctgctgtttg
     9061 ccatggtaga gattgaatct gaagaacggt ggaatgcggt agccagtact gatgtttgcc
     9121 aacgttggtg gaaatatatg accgatgtta tgcccgctaa cccagataac agcccggtga
     9181 gtagcgagct gcaagaagtg ttttacctgc cgtaattgcc caatgtgccg gagatgcctc
     9241 cggcacaata ttttaaattt cgatttgaaa aattaaacat attacatctc ccggtaagtt
     9301 atatttccct gatacattgt gagtaaatca caaaaataat gaataaccca ttaatgattc
     9361 atgtggttta tttaaataac cattatgtgc attactccgc aaatctgacc tttcactctg
     9421 tctcaaattg tcattgttta ccgacatatc gcctctcatc acttaatcgc tccaccatta
     9481 caattgatta aaatatattt tatggagtcc gattaatggc agctcttact gcaagctgta
     9541 ttgacctgaa tattcagggc aatggcgctt attccgttct gaagcagttg gcgacaatag
     9601 cgttacaaaa cggttttatc accgactcac accagttcct gcaaaccctg ctgctgcgcg
     9661 aaaaaatgca ctctactgga tttggttccg gtgtcgcagt gccgcacggt aaaagcgcct
     9721 gcgttaaaca accgttcgta ttattcgccc gcaaagcgca ggctattgac tggaaagcca
     9781 gcgatggcga agacgtcaat tgctggatct gcctcggcgt gccgcaaagc ggcgaagagg
     9841 atcaggtcaa aatcatcggc acactgtgtc gcaaaattat tcaccaggaa tttattcatc
     9901 aactgcaaca gggcgatacc gaccaggtgc tggccttgtt aaatcaaacc ctcagctcat
     9961 aaggaagtgg cgatggagtc atccttacgt attgtcgcga tcaccaactg ccccgccggg
    10021 atcgctcaca cctacatggt ggcggaagcc ctggaacaga aagcccgttc tctcggccat
    10081 accataaaag tggaaactca agggtccagt ggcgttgaaa accgcttatc cagcgaagag
    10141 attgccgctg ccgattacgt cattcttgct accgggcgtg gcctgagcgg tgatgatcgc
    10201 gcgcggtttg ccgggaagaa agtttatgag attgccatct ccctggcgtt gaaaaatatc
    10261 gaccagattt tcagcgaatt accgacaaac tcgcagcttt ttgccgcaga tagcggcgtg
    10321 aagcttggca aacaggaagt gcaaagcggc agcgtgatga gccacctgat ggcgggcgtc
    10381 tctgcggcgc tgccgtttgt catcggcggt ggtatcctgg tggcgcttgc caacatgctg
    10441 gtgcagttcg gtttgccgta taccgatatg tcgaaaggcg cgccgtcgtt tacctgggta
    10501 gttgaatcca tcggttacct tggcttcacc tttatgatcc ccatcatggg cgcttatatt
    10561 gcctcgtcga ttgccgataa accggctttc gctccggcgt ttctggtttg ctatctggcg
    10621 aacgacaaag cgctgctcgg cacccagtcg ggtgcgggat tcctcggcgc ggtggtgctg
    10681 gggctggcga ttggctattt cgtcttctgg ttccgcaaag tgcgccttgg caaagcatta
    10741 caaccgctgc tcggttcaat gctgatcccg ttcgttactc tgctggtttt cggcgtgctc
    10801 acttactacg ttatcgggcc ggtgatgtcc gacctcatgg gcgggctgct ccacttcctg
    10861 aataccattc ctccatcaat gaagtttgcg gcagcgtttc tggtcggtgc gatgctggcg
    10921 ttcgatatgg gcggccccat caataaaacc gcctggttct tctgcttctc actgctggaa
    10981 aaacacattt atgactggta cgccatcgtc ggcgttgtcg cgttgatgcc gcccgttgcg
    11041 gcgggtcttg cgacgttcat tgcgcccaaa ctgtttaccc gacaggaaaa agaggctgcc
    11101 agtagtgcca ttgtggtcgg tgccacagtc gcaaccgaac cggctatacc ttacgctctg
    11161 gcagctccgt tgccgatgat taccgccaat acgctggcgg gcggcattac cggcgtgctg
    11221 gtgatcgctt tcggaatcaa acgtctggca cctggtttgg gtatctttga cccgctgatc
    11281 ggcctgatgt cgccggtagg ttcgttttat ctggtgctgg cgattggcct cgcgctgaac
    11341 atttcattca ttatcgtcct gaaaggattg tggttacgcc gcaaagcgaa agccgcgcaa
    11401 caggagcttg tccatgaaca ttgagttact gcaacagttg tgcgaagcca gcgccgttag
    11461 cggcgatgaa caggaagttc gcgacatcct gataaacacg ctggaacctt gcgttaatga
    11521 aatcaccttt gatggtctgg gcagctttgt tgcccgtaag ggaaataaag gtccaaaagt
    11581 tgccgttgtc gggcatatgg atgaagtcgg ctttatggtc acccacatcg acgagagcgg
    11641 ttttctgcgt ttcaccacca ttggtggctg gtggaatcag tcgatgctca accatcgggt
    11701 aaccatacgc acacacaagg gagtgaaaat ccctggtgtg attggtttcg tcgcgcctca
    11761 tgcgttaacg gaaaagcaaa ggcaacagcc gctgtcattt gatgagatgt tcattgatat
    11821 tggcgcaaac agtcgcgatg aggtggaaaa acgcggtgtg gcgatgggcg attttattag
    11881 cccggaagcc aattttgcct gctggggcga agataaagtg gtcggcaagg cgctggataa
    11941 ccgcatcggc tgcgcaatga tggctgaact attgcagacg gtgaataatc ccgaaattac
    12001 gctgtatggc gtcggcagcg tggaagaaga agttgggcta cgcggggcac aaacctcggc
    12061 ggaacacatt aaaccggatg tagtgatcgt gctggatacc gctgtcgcag gcgatgttcc
    12121 gggcattgat aacattaaat acccgctgaa actgggccag gggccggggc tgatgctgtt
    12181 tgacaagcgc tacttcccca accagaaact ggtagcagcg ttaaaaaact gtgccgcaca
    12241 taacgattta ccgctgcaat tttccaccat gaaaaccgga gcgacggatg gcgggcgcta
    12301 caacgtgatg ggcggcgggc gtccggttgt tgcgctgtgc ctgccaaccc gctatctgca
    12361 cgctaacagc ggcatgattt caaaagccga ttacgatgct ctgctcacgt tgatacgggg
    12421 ttttctgacg accttaaccg cggagaaagt caacgcgttt agccagttcc gtcaggtgga
    12481 ttaatgctaa acgaacgcca gttaaagatt gtcgatctgc tggagcaaca gccgcgcacg
    12541 cctggtgagc tggcgcaaca gactggcgtt tcaggcagga ccatcctgcg tgatattgac
    12601 tatctcaact ttacccttaa cggcaaagcc cgcattttcg ccagtggcag tgcgggctat
    12661 cagctggaaa tcttcgagcg ccgcagcttt tttcagttgc tgcaaaagca cgataacgac
    12721 gatcggctgc tggcgctgtt attactgaat actttcactc cccgtgcgca actcgcctcg
    12781 gcgcttaatt tgccagaaac gtgggtagca gagcgtctgc cccagttaaa acagcgttat
    12841 gaacgcactt gttgcctggc cagccgccct ggtttgggcc atttcattga tgagacagaa
    12901 gagaaactcg ttatcttgct ggcgaacttg ctgcgcaaag atccgttttt aattccgctg
    12961 gcgggcataa cacgagacaa ccttcagcat ttatccacgg cctgcgacaa ccaacaccgc
    13021 tggccgctca tacagggtga ttatctctcc agcctgattc tggcgattta cgccctgcgt
    13081 aatcaactga ccgatgagtg gccgcaat
//
LOCUS       DAJTRC010000097        13086 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.097, whole genome shotgun sequence.
ACCESSION   DAJTRC010000097 DAJTRC010000000
VERSION     DAJTRC010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 13086)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13086)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13086
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.097"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..880)
                     /locus_tag="OO357_003165"
     CDS             complement(<1..880)
                     /locus_tag="OO357_003165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5888013.1"
                     /translation="MKATSEELAIFVSVVESGSFSRAAEQLGQANSAVSRAVKKLEMK
                     LGVSLLNRTTRQLSLTEEGERYFRRVQSILQEMAAAESEIMETRNTPRGLLRIDAATP
                     VVLHFLMPLIKPFRERYPEVTLSLVSSETIINLIERKVDVAIRAGTLTDSSLRARPLF
                     NSYRKIIASPDYISRYGKPETIDDLKQHVCLGFTEPASLNTWPIACSDGQLHEVKYGL
                     SSNSGETLKQLCLSGNGIACLSDYMIDKEIARGELVELMADKVLPVEMPFSAVYYSDR
                     AVSTRIRAFIDFLSEHV"
     gene            1121..1921
                     /locus_tag="OO357_003166"
     CDS             1121..1921
                     /locus_tag="OO357_003166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HCR5888014.1"
                     /translation="MRKNTYAMRYVAGQPAERILPPGSFASIGQALPPGEPLSTEERI
                     RILVWNIYKQQRAEWLSVLKNYGKDAHLVLLQEAQTTPELVQFATANYLAADQVPAFV
                     LPQHPSGVMTLSAAHPVYCCPLREREPILRLAKSALVTVYPLPDTRLLMVVNIHAVNF
                     SLGVDVYSKQLLPIGDQIAHHSGPVIMAGDFNAWSRRRMNALYRFAREMSLRQVRFTD
                     DQRRRAFGRPLDFVFYRGLNVSEASVLVTRASDHNPLLVEFSPGKPDK"
     gene            1999..2769
                     /locus_tag="OO357_003167"
     CDS             1999..2769
                     /locus_tag="OO357_003167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5888015.1"
                     /translation="MTTQSHHDHVEKQFSSQACEYLTSTVHASGRDLQRLAVRLADYP
                     GASALDMGCGAGHASFVAAQNVSAVVAYDLSAQMLDVVAQAAEARQLKNITTRQGYAE
                     SLPFADNAFDIVISRYSAHHWHDVGAALREVNRILKPGGRMIVMDVMSPGHPVRDIWL
                     QTVEALRDTSHVRNYASGEWLRLINEANLIVDNLITDKLPLEFCSWVARMRTPEALVD
                     AIRIYQQSASTEVRTYFALQNDGSFTSDIIMVEAHKAA"
     gene            complement(2817..4163)
                     /gene="mltD"
                     /locus_tag="OO357_003168"
     CDS             complement(2817..4163)
                     /gene="mltD"
                     /locus_tag="OO357_003168"
                     /EC_number="4.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706152.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein transglycosylase D"
                     /protein_id="HCR5888016.1"
                     /translation="MKAKAILLASVLLVGCQSTGNVQQHAQSLSAAGQGEAAKFTSQA
                     RWMDDGTSIAPDGDLWAFIGDELKMGIPENDRIREQKQKYLRNKSYLHDVTLRAEPYM
                     YWIAGQVKKRNMPMELVLLPIVESAFDPHATSGANAAGIWQIIPSTGRNYGLKQTRNY
                     DARRDVVASTTAALNMMQRLNKMFDDDWLLTVAAYNSGEGRVMKAIKTNKARGKSTDF
                     WSLPLPQETKQYVPKMLALSDILKNSKRYGVRLPTTDESRALARVHLSSPVEMAKVAD
                     MAGISVSKLKTFNAGVKGSTLGASGPQYVMVPKKHADQLRESLASGEIAAVQSTLVAN
                     NTPLNSRVYTVRSGDTLSSIASRLGVSTKDLQQWNKLRGSKLKPGQSLTIGAQRLANN
                     SDSITYRVRKGDSLSSIAKRHGVNIKDVMRWNSDTANLQPGDKLTLFVKNNNMPDS"
     gene            complement(4235..4990)
                     /gene="gloB"
                     /locus_tag="OO357_003169"
     CDS             complement(4235..4990)
                     /gene="gloB"
                     /locus_tag="OO357_003169"
                     /EC_number="3.1.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase"
                     /protein_id="HCR5888017.1"
                     /translation="MNLNSIPAFDDNYIWVLNDEAGRCLIVDPGDAEPVLNAITANNW
                     QPEAIFLTHHHHDHVGGVKELVEKFPQIVVYGPQETQDKGTTQVVKDGETAFVLGHEF
                     SVITTPGHTLGHICYFSKPYLFCGDTLFSGGCGRLFEGTALQMYQSLKKLSALPDDTL
                     VCCAHEYTLSNMKFALSIFPHDLSINDYYRKVKELRAKNQITLPVILKNERQINVFLR
                     TEDIDLINGINEETLLQQPEERFAWLRSKKDRF"
     gene            5024..5746
                     /locus_tag="OO357_003170"
     CDS             5024..5746
                     /locus_tag="OO357_003170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414749.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5888018.1"
                     /translation="MKPARVPQTVVAPDCWGDLPWGELYRKALERQLNPWFTKMYGFH
                     LLKIGNLSAEINCEACAVSHQVNVSAQGMPVQVQADPLHLPFADKSVDICLLAHTLPW
                     CTDPHRLLREADRVLIDDGWLVISGFNPISLMGLRKLVPVLRKTSPYNSRMFTLMRQL
                     DWLSLLNFEVLHASRFHVLPWNKHGGKLLNAHIPALGCLQLIVARKRTIPLTLNPMKQ
                     SKNKPRIRQAVGATRQCRKPQA"
     gene            complement(5743..6210)
                     /gene="rnhA"
                     /locus_tag="OO357_003171"
     CDS             complement(5743..6210)
                     /gene="rnhA"
                     /locus_tag="OO357_003171"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HCR5888019.1"
                     /translation="MLKQVEIFTDGSCLGNPGPGGYGAILRYRGREKTFSAGYTRTTN
                     NRMELMAAIVALEALKEHCEVILSTDSQYVRQGITQWIHNWKKRGWKTADKKPVKNVD
                     LWQRLDAALGQHQIKWEWVKGHAGHPENERCDELARAAAMNPTLEDTGYQVEV"
     gene            6275..7006
                     /gene="dnaQ"
                     /locus_tag="OO357_003172"
     CDS             6275..7006
                     /gene="dnaQ"
                     /locus_tag="OO357_003172"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HCR5888020.1"
                     /translation="MSTAITRQIVLDTETTGMNQIGAHYEGHKIIEIGAVEVVNRRLT
                     GNNFHVYLKPDRLVDPEAFGVHGIADEFLLDKPTFAEVADEFMDYIRGAELVIHNAAF
                     DIGFMDYEFSLLKRDIPKTNTFCKVTDSLAVARKMFPGKRNSLDALCARYEIDNSKRT
                     LHGALLDAQILAEVYLAMTGGQTSMAFAMEGETQQQQGEATIQRIVRQASKLRVVFAT
                     DEELAAHEARLDLVQKKGGSCLWRA"
     gene            7139..7215
                     /locus_tag="OO357_003173"
     tRNA            7139..7215
                     /locus_tag="OO357_003173"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7173..7175,aa:Asp,seq:gtc)
     gene            complement(7346..8392)
                     /locus_tag="OO357_003174"
     CDS             complement(7346..8392)
                     /locus_tag="OO357_003174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImpA family type VI secretion system protein"
                     /protein_id="HCR5888021.1"
                     /translation="MEIFLSDPISAECPCGPDLEYDPEYLLLFTRAAPREEAQYGDFV
                     STPENINWAELERDAHRLLMRSKDIRILVVLLRCRIQQAGARGLSEALTLLETLCSTY
                     PDAIHPQLLATEDITAEDAAVARSNALAALLDHEGVMADIRGITLSNNAAMRLQVRDV
                     ERSLSALRPADALAPESVRQQLADLEARGTLPLDAFRQAAETTERLQRHARETLNDQA
                     PDFSRLTQLLALLPGAVQSTTPEILPQPQAEQPENATIVHTEQMQAEQIALPVHIAEE
                     ISPMTGAEPRQIRDRNDALERLRVIRRWFEHSEPSSPTIPLLRQAERLVGKRVSEVIN
                     EIPVELLEKWDALE"
     gene            complement(8406..8965)
                     /gene="tssG"
                     /locus_tag="OO357_003175"
                     /pseudo
     CDS             complement(8406..8965)
                     /gene="tssG"
                     /locus_tag="OO357_003175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001601590.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
     gene            complement(8962..10845)
                     /gene="tssF"
                     /locus_tag="OO357_003176"
     CDS             complement(8962..10845)
                     /gene="tssF"
                     /locus_tag="OO357_003176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HCR5888022.1"
                     /translation="MDPRLLEYYNRELSYLRETGAEFAARHPKVAARLGMQGTDIADP
                     YVERMVEAFSFLTARTQLKIDAEFPRFTQRLLEVVSPNYVTPTPSMSVAQLHPDTEEG
                     DLAKGFTVPRDTAFFSAIPEGESTACQFRSSQDVTLWPLAIEEARLTAAPPDMPALHR
                     YLPANIHVAGALRITLRTFGELTFSQLAGLDRLPFYLCGEERTASHLLELLHTSAIAP
                     LAGIPGHFDGALDVNLQQPVMYEGLEPDQGLLPLAWNVFHGHNLLHEYFACPERFYFF
                     TPTGLSAGLQKIDGGVAEIVILLNRLPPDWLIHQTNAAQFSLFCTPVINLFPRVTARI
                     DVTHSTTEQHLVVDRTHPLDYEVFSVQEVEGLETDTTRKMAFRPLYHTRNNDEGNHGR
                     YFSLRREPRRLSENARRYGTRTPYTGSEVFLSLVDQYEAPYPENLRHITITAMVTNRD
                     LPCLIARNGRDDLTVDAAIPVAGVGLIRPPRSPQPPMAEREMAWRLIRQLSFNYLPLA
                     DLDHRTGGQALRDLLNLFIPAHDSPQSRQVRSLIGCKTTPVTRRLPGSGLLVYGRGVS
                     CELTVDEEGFSGISPYLFGLVLEHYIARHVSINTFSQMTLHSMQRGKIMTWPVRAGQR
                     GSV"
     gene            complement(10861..11355)
                     /gene="tssE"
                     /locus_tag="OO357_003177"
     CDS             complement(10861..11355)
                     /gene="tssE"
                     /locus_tag="OO357_003177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706160.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssE"
                     /protein_id="HCR5888023.1"
                     /translation="MSASRQRPGNHLLPTLFDRLCDDAPNQKRDHGISVSPVQIKEII
                     RRDLSFLLNTVSHEDDIDAARYPYAAASVLNYGLPPLAGSFLYEHKWDDIRRAILRTI
                     TRFEPRLKASTLQIIPLQDERRQSGHNTLQFEIRGEILTQPYPTAFRVRSALDMEQSR
                     ITFF"
     gene            complement(11352..12182)
                     /locus_tag="OO357_003178"
                     /pseudo
     CDS             complement(11352..12182)
                     /locus_tag="OO357_003178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016233830.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="impE family protein"
     gene            complement(12169..13083)
                     /gene="tagK"
                     /locus_tag="OO357_003179"
                     /pseudo
     CDS             complement(12169..13083)
                     /gene="tagK"
                     /locus_tag="OO357_003179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000804008.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagK"
BASE COUNT         3006 a         3392 c         3479 g         3209 t
ORIGIN      
        1 ttacatgctc gctaaggaaa tcgataaaag cccggatgcg cgtacttacc gcacggtcgc
       61 tgtaatagac ggcactgaat ggcatttcca ctggcaacac tttatctgcc attaactcca
      121 ccaattctcc gcgagcgatt tctttgtcga tcatgtagtc ggacaaacac gcaatcccgt
      181 tgccactcag gcaaagctgt ttcagtgttt ccccactatt ggatgacaaa ccgtacttca
      241 cctcatgtaa ttgtccatca ctacaggcta tcggccaggt attgagggaa gcgggttcag
      301 tgaatcccag gcaaacatgt tgctttaaat cgtcgatcgt ttctggcttc ccgtagcggg
      361 aaatataatc gggggaggcg ataatttttc gataactgtt aaataacggc ctggcacgta
      421 agctggagtc cgttaacgta ccagcgcgta tcgcgacatc cacttttctt tcgatcaaat
      481 taataatcgt ttcggaggag actagcgata aagtgacttc cggatagcgt tcacggaaag
      541 gcttaattaa cggcatcaga aagtgcagca ccactggagt tgcggcatcg atccgtaaca
      601 gtccacgcgg tgtattacgc gtctccataa tttctgattc tgccgctgcc atctcctgca
      661 aaattgactg tacgcgacga aaataacgct cgccttcttc cgtcaggcta agttgccgcg
      721 tggtccgatt aagcaggcta acgccaagtt tcatttccag ctttttcacc gcccggctta
      781 ccgctgagtt tgcttgccct aattgttccg ctgcccggct aaagctgccg ctttcgacga
      841 ccgaaacaaa aatggcgagt tcttccgacg tggctttcat ttttgctcct gttgcaaaat
      901 agatgagata ttttgaattt atttgtcatt aaaccatcag gatgcgtgat atgtcatccg
      961 atttaatgtt ctcaataatg agcaaatttc tgacaggtgt aagcacttgc ttacataaca
     1021 atatacaatt gctcgttgaa agagtgagct aaaatcccta taacagtaga accctcccga
     1081 gtgcggaagg gttgacgtaa tagaggtttc aaagtcaaaa gtgcgaaaaa acacctatgc
     1141 catgcgctat gttgccggac aacctgcgga aaggatctta ccgccggggt cttttgcgag
     1201 catcggccag gcattaccac ctggagaacc gttaagtacc gaagagcgta ttcggatcct
     1261 ggtgtggaac atatacaaac agcaacgcgc tgaatggttg tcggtattaa agaactacgg
     1321 caaagatgca catctggtgt tattgcagga agcgcagaca acgccagagt tagtacagtt
     1381 tgcgaccgct aactatcttg ccgccgatca ggtgcccgct ttcgtgctgc cacaacatcc
     1441 ttccggtgta atgacccttt cggcggcgca tccagtgtat tgctgcccgt tacgtgaacg
     1501 agaacccatt ttgcgtctgg cgaagtcggc actggtgacg gtctatccat tacctgacac
     1561 ccgcctgttg atggtggtta atatacacgc cgtcaacttc agtctgggcg tggatgtcta
     1621 tagtaagcag ttacttccta ttggtgatca gatagctcac cacagcggcc cggtcattat
     1681 ggcgggagat ttcaatgcct ggagccgtag aaggatgaac gcgttatatc gctttgcgcg
     1741 ggaaatgtcg ctgcgccagg tgcgttttac cgatgatcag cgccgtcggg cgtttggccg
     1801 cccgctcgat tttgttttct accgtggtct gaacgtcagc gaagcttctg tattggttac
     1861 gcgcgcttcc gatcacaatc cgctactcgt tgaattcagt cccggcaagc ctgataaata
     1921 aggtatgtca ggtctgccac agggcagacc aacgtttggc gctgtccttt tatactcaaa
     1981 caacaaaagg acagcgcaat gacaacacaa tcccaccatg accacgtaga aaagcagttt
     2041 agctctcaag cctgcgaata tctgaccagt accgtacatg catccggacg agatttacag
     2101 cgcctggcgg tgcgtctggc tgattatcct ggtgcaagtg cacttgatat gggctgcgga
     2161 gcagggcatg ccagctttgt tgctgcgcaa aacgtgagcg cggtggtggc gtatgactta
     2221 tctgcccaaa tgctggatgt cgtggcacaa gctgccgaag cccggcaact gaaaaatatc
     2281 accacccgcc agggatatgc cgaaagtctg ccatttgccg ataacgcatt tgatattgtt
     2341 atcagccgtt attctgccca tcactggcat gatgttggtg cagcactgcg tgaagtgaac
     2401 cggatattga aacctggcgg taggatgatt gtgatggacg taatgtctcc gggtcatcca
     2461 gtgcgcgaca tctggttaca gacggtagaa gcattacgcg atacctctca cgtacgaaac
     2521 tacgccagcg gtgagtggtt gaggttaatc aatgaagcca atctgatagt tgataattta
     2581 attacagata agttaccgct ggaattttgt tcatgggtcg cgagaatgcg tacgccagaa
     2641 gcgttagtag acgctattcg catttaccaa cagagcgcat cgacagaggt gagaacgtat
     2701 tttgccttgc agaatgacgg ctctttcacc agtgatatca tcatggtaga agcacataaa
     2761 gcggcataaa taaaaaaggc accgggggaa tcggtacctt tttattatct ggtttgtcag
     2821 gaatctggca tgttgttgtt tttcacaaac aacgtcagct tgtcgcctgg ttgcagattc
     2881 gcagtgtcgc tgttccagcg catcacatct ttgatgttca cgccgtggcg tttagcaatg
     2941 cttgaaagcg aatcgccttt gcgcacacga tacgtaatgc tatcgctgtt gtttgccagt
     3001 cgctgtgcac caatcgtcaa actttggcct ggcttcagct tagatccgcg cagtttgttc
     3061 cactgctgca aatctttggt gcttacgccg agacgtgaag cgatgcttga aagcgtgtcg
     3121 ccagagcgta cggtgtaaac acggctgtta agcggcgtat tgttggcaac cagcgtcgac
     3181 tgtacagcag caatttcgcc tgaagcgaga gattcacgca gttgatctgc atgcttcttt
     3241 ggcaccatca cgtactgcgg cccacttgcg cccagcgtgg agcctttcac gccagcgttg
     3301 aatgtcttca gcttgctgac ggaaatcccc gccatatctg caaccttcgc catttcaacc
     3361 gggctactca ggtgcacacg cgccagagca cggctttcat cggtcgttgg cagacgtacg
     3421 ccataacgct tgctgttttt gagaatatca ctcaatgcca gcattttagg cacgtactgc
     3481 ttcgtttcct gcggcaacgg taacgaccag aagtccgtgg atttcccacg cgctttgttc
     3541 gttttaattg ccttcatgac ccgaccttcg ccgctgttat aagccgctac ggtcagaagc
     3601 cagtcgtcgt caaacatttt gttcagacgc tgcatcatat tcagcgcggc agttgttgaa
     3661 gcaacaacat cgcgacgcgc gtcataattg cgggtctgtt tcaaaccata attgcgcccc
     3721 gtgctcggaa tgatctgcca gatgcctgcg gcattggcgc cagacgttgc gtgaggatca
     3781 aaagcgctct ccactatggg tagtagtacc agttccatag gcatgttacg ttttttaact
     3841 tgccctgcta tccagtacat atacggctct gcccgtaaag ttacatcgtg gagatagctc
     3901 ttattgcgta aatatttctg tttctgttcg cgaatccggt cattttccgg aattcccatc
     3961 tttagctcgt cgccaatgaa agcccacaag tcaccatctg gcgcgataga cgtcccatcg
     4021 tccatccatc gtgcctgact tgtaaacttt gctgcttccc cttgaccagc tgcagaaagg
     4081 ctctgtgcgt gctgttgaac gttgccggta ctctggcaac ccacgagcag gacagaggcg
     4141 agtaatatcg cttttgcctt catgtgtgtg tcaatagttg cttaaaagac gaccgatcat
     4201 aacggcgaac ggagccgatg acaagaaagt tttatcagaa cctatctttc tttgacctta
     4261 accatgcaaa acgctcttca ggttgttgca ataatgtttc ttcattaatt ccattaatta
     4321 aatcaatatc ttccgttctt aaaaaaacat taatttgccg ctcatttttc agaattacgg
     4381 gtagtgttat ttgatttttt gcccgtaact ccttaacttt acgataataa tcatttatgg
     4441 acaaatcgtg cggaaaaata ctcaaagcaa acttcatatt tgataacgta tattcatgag
     4501 cacaacatac caatgtatcg tcaggtaacg cacttagctt tttaagtgat tgatacattt
     4561 gtaaagctgt cccttcaaac aaccgaccac acccaccaga aaacagcgtg tcgccgcaaa
     4621 atagataagg tttactgaag taacagatat gtcctaaagt gtgacccggt gtggtaatta
     4681 cactaaattc atgccctaaa acgaaggcag tttcgccatc tttgactacc tgtgttgttc
     4741 ccttatcttg tgtctcttgt ggaccataca ctacaatttg cggaaacttt tccaccagtt
     4801 cttttacgcc gccaacgtga tcgtggtgat ggtgggttag aaatatggcc tccggttgcc
     4861 agttattggc ggtaatggcg tttaatactg gctctgcgtc tccgggatcg acaatcaggc
     4921 agcgacctgc ctcatcattc aaaacccaga tgtaattgtc atcaaaggcg ggaatactgt
     4981 taagattcat agattacctc tcagtgtaaa acggaaggtt gtgatgaaac cggcaagagt
     5041 ccctcaaact gtcgtggctc ctgattgctg gggcgatttg ccctggggag agctttatcg
     5101 caaggcgctg gagcgccagc tcaacccgtg gttcactaaa atgtatggtt ttcatctgct
     5161 taagattggc aatttaagcg cagaaatcaa ttgcgaagcg tgcgcggttt ctcatcaagt
     5221 gaatgtttct gcgcaaggaa tgcccgtcca ggtgcaggcg gatccacttc atcttccttt
     5281 tgccgataaa tccgttgata tttgtctact ggcacatacc ttgccgtggt gcaccgatcc
     5341 gcatcgttta ttacgtgaag ccgatcgggt actgattgat gatggctggc tggtgattag
     5401 tggcttcaac cccatcagtt taatgggatt acgcaaactt gtgccggtat tgcgcaaaac
     5461 ctcgccctat aatagccgga tgtttactct gatgcggcaa ctggactggc tctctttgtt
     5521 gaattttgaa gtgctgcacg ccagccgttt ccacgttctc ccgtggaaca aacacggagg
     5581 aaaactattg aatgcgcata ttcctgcgct tggttgctta caacttattg ttgcccggaa
     5641 acggactatt cctttaacgc taaatccgat gaaacagagt aaaaacaagc cacgaattcg
     5701 ccaggcggtt ggagccaccc ggcaatgtcg taaaccacag gcttaaactt cgacttggta
     5761 gcccgtatct tccagtgtgg gattcatcgc cgcggcacga gccagttcat cacagcgttc
     5821 gttttccggg tgtccggcat ggcctttaac ccattcccat ttgatttgat gctgccccaa
     5881 tgcagcatca agacgttgcc agagatcgac attttttact ggttttttgt ctgctgtttt
     5941 ccagccacgt tttttccagt tatggatcca ctgggtgata ccctggcgga catactggct
     6001 gtcggtactc aaaatgactt cgcaatgttc ttttaacgcc tccagcgcga caatagcggc
     6061 catcaactcc atacggttgt tggtggtgcg ggtgtagcct gcgctaaagg ttttctcgcg
     6121 tccgcgatag cgtaaaatag cgccgtaacc cccaggtcca ggattaccca gacacgaacc
     6181 atcggtgaaa atttctacct gtttaagcat ctctggtaga cttcctgtaa ttgaatcgaa
     6241 ctgtaaaacg acaagtctga cataaatgac cgctatgagc actgcaatta cacgccagat
     6301 cgttctcgat accgaaacca ccggtatgaa ccagattggt gcacactatg aaggccacaa
     6361 gatcattgag attggtgctg ttgaagtagt gaaccgtcgc ctgacgggca ataacttcca
     6421 tgtttatctc aaacccgatc ggctggtgga tccggaagcc tttggcgtac atggtattgc
     6481 tgatgaattt ttgctcgata agcccacgtt tgccgaagta gccgatgagt ttatggacta
     6541 tatccgcggc gcggagttgg tgatccataa cgcagcgttc gatataggct ttatggacta
     6601 cgagttttcg ttgcttaagc gcgatattcc gaagaccaat accttctgta aggtcaccga
     6661 tagccttgcg gtggcgagga aaatgtttcc cggtaaacgc aacagcctcg atgcgctatg
     6721 tgcccgctac gaaatagata acagtaaacg aacgctgcac ggggcattac tcgatgccca
     6781 gatccttgcg gaagtttatc tggcgatgac cggtggtcaa acgtcgatgg cttttgcgat
     6841 ggaaggagag acacagcagc aacaaggtga agcaacaatt cagcgcattg tacgtcaggc
     6901 aagtaagtta cgcgttgttt ttgcgacaga tgaagagctg gcagcacatg aagcccgtct
     6961 cgatctggtg cagaaaaaag gcggaagctg cctctggcgg gcataaatac atgtaaaagg
     7021 cgctaaaaat agcgacttgg gcgatttttg cagcaaacga ttcaaaagat gagaaaaatc
     7081 gttgacgaag gtcgaggcaa tccgtaatat tcgcctcgtt cccaacggaa cacaacgcgg
     7141 agcggtagtt cagtcggtta gaatacctgc ctgtcacgca gggggtcgcg ggttcgagtc
     7201 ccgtccgttc cgccaatatt cagaaagccc gaatcagtaa tgattcgggc tttcgtcgtt
     7261 ttggtgaagt gagaattttt cactccgaag taaaacggat atgatttaaa aaatggccag
     7321 acattataaa ttctggccac ttctgttact ccagcgcatc ccatttctcc agcagttcta
     7381 ctggaatttc atttattacc tccgagacgc gttttccgac caggcgttct gcctggcgca
     7441 gcaaaggaat ggtcgggctg ctcggttcgc tgtgctcaaa ccagcgacgg atgacacgca
     7501 ggcgttccag cgcgtcgttt cggtcgcgga tctgccgggg ttctgcgccg gtcatcggtg
     7561 atatttcctc cgcgatatgc accggtagcg caatctgctc tgcctgcatc tgttcagtat
     7621 gaacgatggt ggcgttttcc ggctgctcag cctgtggttg tggcaggatc tccggcgtgg
     7681 tgctctgcac tgctcccggt aacagggcca gcagttgcgt cagacgggag aagtccggtg
     7741 cctggtcatt cagcgtttca cgcgcatggc gttgcagccg ctcagtggtc tctgctgcct
     7801 ggcgaaaggc atccagcggc agtgtgccgc gcgcttccag gtctgccagt tgctggcgta
     7861 ccgattctgg tgccagcgca tccgccgggc gtaatgcaga tagcgaacgc tccacatccc
     7921 gcacctgaag gcgcatggca gcattatttg acagtgtaat gccacgaata tccgccatta
     7981 caccttcgtg gtccagcaac gccgccagcg cgttactgcg tgccaccgca gcatcttcgg
     8041 cggtgatatc ttcagtcgcg agcagttgag gatggatggc gtccgggtac gtggagcaca
     8101 acgtttccag cagcgtgagc gcctccgaca gtcccctggc accggcctgc tggatacggc
     8161 agcgcagcag gacaaccaga atgcggatat ctttgctgcg catcagcaga cgatgggcgt
     8221 cccgctcaag ctccgcccag ttaatgtttt ccggcgtact gacaaaatcg ccatattgcg
     8281 cttcttcacg cggtgcggcg cgggtgaaca gcagcaggta ttccggatcg tactccagat
     8341 ccgggccgca ggggcattcc gccgaaattg ggtcagacag gaatatttcc atttaattat
     8401 cctcagtgcg ccagcgtggt ggccagaccg tccggattgc tggactcacg caccagatgg
     8461 gccgatgccg ccagacgagc gtgatctggc aacgggctgt cagcgctctc ccgcaaatcc
     8521 tgcccgctga ggctggcgac ataaaaggca aagcggtcat gttgcggttt atccagaccg
     8581 cccccagcgg actgggcgct gcgccatgcc tggtaaaact gcgtcagcca gcggtgatga
     8641 aaaagatcag aaaaacgggt cagcgtcggc tttcagtccg gttacgcgcc agttcggtgt
     8701 agtgcagcgg cagtggaccg ttcggccccc acagcacgag gctgtacaga ctgatgtgca
     8761 ggcgcccttc ctgccagccc acactggcga tttctcgtgg cgcaaaggtc agcgcgggcg
     8821 tttgtccgag gcgaaatttt tccatccgtg gttgccaggt gttgcccgcc ggaacggtac
     8881 acaactgcac atccacgcgg cgcatcaggt tcagaaaacc gtaacgccag gggggggcct
     8941 gcgcctgagt gaaatcgttg ctcatacgct tccccgctgt cccgccctga caggccaggt
     9001 cataattttg ccgcgctgca tgctgtgcag cgtcatctgc gagaaggtat taatggaaac
     9061 atggcgggcg atatagtgtt ccagcaccag gccgaacaga tacggactga tgccggaaaa
     9121 accttcttca tccaccgtca gttcacaact gacgccacgc ccatagacca gtagtccgga
     9181 accgggcaga cggcgggtga ccggggtcgt tttacaacca atcaggctgc gcacctggcg
     9241 cgattgcgga ctgtcatgag caggaataaa caggttcagc agatctcgca acgcctggcc
     9301 gccggtacgg tgatccagat cggccagcgg cagataatta aaagataact ggcggatcaa
     9361 ccgccatgcc atctcacgtt cagccatcgg gggctgcggc gagcgtggcg ggcggataag
     9421 ccctacgccc gccaccggga tggctgcatc cacggttaga tcgtcccggc cattgcgggc
     9481 aataagacag ggcagatcgc ggttagtgac catggccgtg atggtgatgt ggcgcagatt
     9541 ttccgggtac ggcgcttcat actggtcaac cagcgacagg aacacttccg agcccgtata
     9601 gggggtacgg gtgccgtaac ggcgggcgtt ttctgataag cggcggggct cccggcgcag
     9661 ggaaaaataa cgtccgtgat tgccttcatc attattgcgg gtgtggtaga gcgggcgaaa
     9721 ggccatcttc cgtgtggtgt cggtttccag cccttccact tcctggaccg agaacacctc
     9781 ataatccagc ggatgggttc tgtccaccac cagatgctgt tccgtcgtgc tgtgcgtgac
     9841 atcgatccgg gcggtgacgc ggggaaacag gttgataacc ggggtacaga acaggctgaa
     9901 ctgtgccgca tttgtctggt ggatcagcca gtccggcggc aggcggttca gcaggataac
     9961 aatctccgcc acgccaccgt caattttctg taatccggca gacaggcctg tcggggtgaa
    10021 aaaatagaag cgttccggac aggcaaaata ttcatgcagc aggttatgac cgtgaaacac
    10081 gttccacgcc agcggcagca gcccctgatc cggctccagg ccttcgtaca taacgggctg
    10141 ttgcagattc acatccagcg cgccgtcaaa atgccccgga atgccagcca ggggcgcgat
    10201 ggcgctggta tgtagaagtt ctaacaggtg tgaggccgta cgctcctcgc cgcagagata
    10261 aaacggcagc ctgtccagtc cggcaagctg actgaacgtt agttcaccaa aggtgcgcag
    10321 ggtaatacgc agcgccccgg cgacgtgaat gttggccggc aggtagcggt gcagggcagg
    10381 catatccggt ggcgcggcgg tcagtcgcgc ctcttcgatg gcgagcggcc acagcgttac
    10441 atcctggctg ctgcgaaact ggcaggcagt gctttcgcct tccggaatgg cggagaaaaa
    10501 ggctgtgtcg cgcgggacgg taaatccttt cgccagatcg ccttcttccg tatcgggatg
    10561 caactgcgcc acgctcattg agggcgtggg tgtgacgtaa ttggggctga ccacttccag
    10621 caggcgctgg gtgaaacggg ggaattcagc gtcaattttt agctgcgtgc gggcggtcag
    10681 gaagctgaag gcctcgacca ttcgctctac atacggatcg gcaatatccg ttccctgcat
    10741 gcccagccgg gcggcgactt tgggatgacg ggcggcaaat tcggctcccg tttcccgcag
    10801 atagctcagt tcgcggttgt agtattccag tagccgtgga tccatcggtc tctctctgaa
    10861 ttaaaaaaac gtaatgcgac tctgctccat atccagcgcg ctacgtaccc ggaatgcagt
    10921 cggatacggc tgcgtgagga tctcgccgcg aatttcaaac tgtaatgtgt tatggccact
    10981 ctggcggcgc tcatcctgaa gtgggatgat ttgcagagtg gacgctttca gccggggttc
    11041 aaaccgggtg atggttcgca aaatggccct gcggatatcg tcccacttgt gctcatacag
    11101 aaagctgccc gccagtggcg gcaggccgta gttaagtacg gacgctgccg cgtacggata
    11161 acgtgcagca tcaatatcat cttcatggct gacggtgttg agcagaaatg acaaatcccg
    11221 gcggatgatc tccttaatct gaaccggact gacgctgata ccgtggtcgc gtttctggtt
    11281 tggggcgtcg tcacacaggc ggtcaaacag cgtgggcagc aggtgattgc ctggacgctg
    11341 gcgggaagcg ctcacgcgtc gcttccttca gcggtgttaa aggtacaggt ggacaaatcc
    11401 agcaggctga tatcgccgtg accggtgagc cacaccctct gacccagcgc ctgtaccagt
    11461 gtctctcccg gaccgtcctg ccagaccgtt tcccggcaca gacgcaggga gtcggcagcc
    11521 ttttcagagc cgctgtagcg gggaaacagc taggcactgt gtgtatcgcc attgttcagg
    11581 gtgacgttga cgggtttcca gatcaaatcg atcagatgcg caggccgcgg cgatttcagc
    11641 gaacagattt gtgaaaacgg cagccagata tatgctccgc ccgcgatcag ttcgagtacc
    11701 ggaccggtac gggagtcgct gtcgctaatc cagtcaaatg cgccgccatt ccactgcccg
    11761 gaagtgtctg ttatggcctc aagcgcgcgg ctgcggtgag catcagcttc ctgcgcttca
    11821 ccacgggcgt tacaggcgag ggcggtcagc agatcctcca tccaggcggg cggcgggaga
    11881 ataacaccgg gccgctgttc gccctgaaag caggcgtggc ggtatatctc acagcggatc
    11941 agctcgccaa atacctgcgc ttcgcgagta taatttgcgt ccatccgggc gcatagctgg
    12001 atctgttgca gtgcccgcga ccagtcgcct gccacgcaca ataactgaaa caggctgtgg
    12061 cggcgggagg cctgcgctgg gtgcatcctg acctgttgct ctgccatctg aatgccctcc
    12121 accacggaat aatcccgcag cagcgccgac aaggtaatcg gtagctcgtt attgtttgtc
    12181 atgatatatc tctccatgtt gtgccgggtt tatccggtaa tggctgtcaa tgccaatgat
    12241 gcgatgctct ttccgtgtca gatctggcag tacggtgtta tgcgctgttt ccggggcgta
    12301 ttccggggaa agtaaatgca ggatatcagg gggagtcttt tcgacaggcc agatagtttc
    12361 atcttcgcca gtggcatcca gtacattgag aatagcgtca atgcttaacc caccagtgac
    12421 catatcctgc aatgtctcgg cttcggtgac caggtcacgc aatgaatcca gatcctgcga
    12481 ggagggctct tcgttaaaag gaaaaggatc agcatggcga ttaagatctt gttcgggaga
    12541 taacaacgcc tcgcggtatt cctgataaag tcgggtcagg gcatttcctt tttccgcacc
    12601 ttctccggtg gaggaggctc catgaacagg aatgatatcg aatggatttt gtgatgtcag
    12661 atgctgctgc ctgaaccaat ccagatccag atattcggtt attgcttccg acgtgatggc
    12721 agtctgcgat atcgttttta ctgtgctcag gttcggagat gatggattgc catgtacaag
    12781 gcactatgaa gataaccccc attctatcag gtcgccttca ttcagacgca tacggtgatg
    12841 aggttccaca atctggtcgt ttaccgtaca gcaatattcg gtagactgat tgataatcca
    12901 ccatgcttct tcgtggcggg tcagagtgag ggcaatcttt acatcatcat tcaccttaaa
    12961 aacaggaatc cagggagagg taacggtgaa gataatttgt gttccttcag tcgcgtaaat
    13021 tgtgttatcg gcgatcccgt gcgcctgtat tttttggagt ccccacgtaa tatttggttt
    13081 cattgt
//
LOCUS       DAJTRC010000098        12981 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.098, whole genome shotgun sequence.
ACCESSION   DAJTRC010000098 DAJTRC010000000
VERSION     DAJTRC010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12981)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12981)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12981
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.098"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(5..1066)
                     /locus_tag="OO357_003180"
     CDS             complement(5..1066)
                     /locus_tag="OO357_003180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HCR5888024.1"
                     /translation="MLEMLMQWYRRRFSDPEAIALLVILVAGFGIIFFFSGLLAPLLV
                     AIVLAYLLEWPTVRLQSIGCSRRWATSIVLVVFVGILLLMAFVVLPIAWQQGIYLIRD
                     MPGMLNKLSDFAATLPRRYPVLMDAGIIDAMAENMRSRMLTMGDSVVKISLASLVGLL
                     TIAVYLVLVPLMVFFLLKDKEQMLNAVRRVLPRNRGLAGQVWKEMNQQITNYIRGKVL
                     EMIVVGIATWLGFLLFGLNYSLLLAVLVGFSVLIPYIGAFVVTIPVVGVALFQFGAGT
                     EFWSCFAVYLIIQALDGNLLVPVLFSEAVNLHPLVIILSVVIFGGLWGFWGVFFAIPL
                     ATLIKAVIHAWPDGQIAQE"
     gene            1279..2742
                     /gene="bepA"
                     /locus_tag="OO357_003181"
     CDS             1279..2742
                     /gene="bepA"
                     /locus_tag="OO357_003181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-barrel assembly-enhancing protease"
                     /protein_id="HCR5888025.1"
                     /translation="MFRQLKKNLVATLIAAMTIGQVAPAFADSADTLPDMGTSAGSTL
                     SIGQEMQMGDYYVRQLRGSAPLINDPLLTQYINSLGMRLVSHANSVKTPFHFFLINND
                     EINAFAFFGGNVVLHSALFRYSDNESQLASVMAHEISHVTQRHLARAMEDQQRSAPLT
                     WVGALGSILLAMASPQAGMAALTGTLAGTRQGMISFTQQNEQEADRIGIQVLQRSGFD
                     PQAMPTFLEKLLDQARYSSRPPEILLTHPLPESRLADARNRANQMRPMVVQSSEDFYL
                     AKARTLGMYNSGRNQLTSDLLDEWAKGNVRQQRAAQYGRALQAMEANKYDEARKTLQP
                     LLAAEPGNAWYLDLATDIDLGQNKANEAINRLKNARDLRTNPVLQLNLANAYLQGGQP
                     QEAANILNRYTFNNKDDSNGWDLLAQAEAALNNRDQELAARAEGYALAGRLDQAISLL
                     SSASSQVKLGSLQQARYDARIDQLRQLQKRFKPYTKM"
     gene            2763..3122
                     /gene="arsC"
                     /locus_tag="OO357_003182"
     CDS             2763..3122
                     /gene="arsC"
                     /locus_tag="OO357_003182"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708333.1"
                     /note="This arsenate reductase requires both glutathione
                     and glutaredoxin to convert arsenate to arsenite, after
                     which the efflux transporter formed by ArsA and ArsB can
                     extrude the arsenite from the cell, providing resistance;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase (glutaredoxin)"
                     /protein_id="HCR5888026.1"
                     /translation="MTKQVKIYHNPRCSKSRETLNLLKENGVEPEVVLYLETPADAAT
                     LRDLLKMLGMNSARELMRQKEDLYKELNLADSSLSEEALIQAMVDNPKLMERPIMVAN
                     GKARIGRPPEQVLEIVG"
     gene            complement(3255..4001)
                     /gene="hda"
                     /locus_tag="OO357_003183"
     CDS             complement(3255..4001)
                     /gene="hda"
                     /locus_tag="OO357_003183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA inactivator Hda"
                     /protein_id="HCR5888027.1"
                     /translation="MVNFSRFCEILVEVSLNTPAQLSLPLYLPDDETFASFWPGDNSS
                     LLAALQNVLRQEHSGYIYLWAREGAGRSHLLHAACAELSQRGDAVGYVPLDKRTWFVP
                     EVLDGMEHLSLVCIDNIECIAGDELWEMAIFDLYNRILESGKTRLLITGDRPPRQLNL
                     GLPDLASRLDWGQIYKLQPLSDEDKLQALQLRARLRGFELPEDVGRFLLKRLDREMRT
                     LFMTLDQLDRASITAQRKLTIPFVKEILKL"
     gene            complement(4051..5340)
                     /gene="uraA"
                     /locus_tag="OO357_003184"
     CDS             complement(4051..5340)
                     /gene="uraA"
                     /locus_tag="OO357_003184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HCR5888028.1"
                     /translation="MTRRAIGVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHINPA
                     TVLLFNGIGTLLYLFICKGKIPAYLGSSFAFISPVLLLLPLGYEVALGGFIMCGVLFC
                     LVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGVAAGMAGLLPAEGQTPNSKTII
                     ISITTLAVTVLGSVLFRGFLAIIPILIGVLVGYALSFAMGIVDTTPIINAHWFALPTL
                     YTPRFEWFAILTILPAALVVIAEHVGHLVVTANIVKKDLLRDPGLHRSMFANGLSTVI
                     SGFFGSTPNTTYGENIGVMAITRVYSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVM
                     GGVSLLLYGVIGASGIRVLIESKVDYNKAQNLILTSVILIIGVSGAKVNIGAAELKGM
                     ALATIVGIGLSLIFKLISVLRPEEVVLDAEDADITDK"
     gene            complement(5426..6052)
                     /gene="upp"
                     /locus_tag="OO357_003185"
     CDS             complement(5426..6052)
                     /gene="upp"
                     /locus_tag="OO357_003185"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001295473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HCR5888029.1"
                     /translation="MKIVEVKHPLVKHKLGLMREQDISTKRFRELASEVGSLLTYEAT
                     ADLETEKVTIEGWNGPVEIDQIKGKKITVVPILRAGLGMMDGVLENVPSARISVVGMY
                     RNEETLEPVPYFQKLVSNIDERMALIVDPMLATGGSVIATIDLLKKAGCSSIKVLVLV
                     AAPEGIAALEKAHPDVELYTASIDQGLNEHGYIIPGLGDAGDKIFGTK"
     gene            6376..7413
                     /gene="purM"
                     /locus_tag="OO357_003186"
     CDS             6376..7413
                     /gene="purM"
                     /locus_tag="OO357_003186"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HCR5888030.1"
                     /translation="MTDKTSLSYKDAGVDIDAGNALVGRIKGVVKKTRRPEVMGGLGG
                     FGALCALPQKYREPVLVSGTDGVGTKLRLAMDLKRHDTIGIDLVAMCVNDLVVQGAEP
                     LFFLDYYATGKLDVDTASAVISGIAEGCLQSGCSLVGGETAEMPGMYHGEDYDVAGFC
                     VGVVEKSEIIDGSKVSDGDVLIALGSSGPHSNGYSLVRKILEVSGCDPQTTELDGKPL
                     ADHLLAPTRIYVKSVLELIEKVDVHAIAHLTGGGFWENIPRVLPDNTQAVIDESSWQW
                     PEVFNWLQTAGNVERHEMYRTFNCGVGMIIALPAPEVDKALALLNANGENAWKIGIIK
                     ASDSEQRVVIE"
     gene            7413..8051
                     /gene="purN"
                     /locus_tag="OO357_003187"
     CDS             7413..8051
                     /gene="purN"
                     /locus_tag="OO357_003187"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HCR5888031.1"
                     /translation="MNIVVLISGNGSNLQAIIDACKTNKIKGTVRAVFSNKADAFGLE
                     RARQAGIATHTLIASAFDSREAYDRELIHEIDMYAPDVVVLAGFMRILSPAFVSHYAG
                     RLLNIHPSLLPKYPGLHTHRQALENGDEEHGTSVHFVTDELDGGPVILQAKVPVFAGD
                     TEDDITARVQTQEHAIYPLVISWFADGRLKMHENAAWLDGQRLPPQGYAADE"
     gene            8223..10289
                     /gene="ppk1"
                     /locus_tag="OO357_003188"
     CDS             8223..10289
                     /gene="ppk1"
                     /locus_tag="OO357_003188"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708339.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HCR5888032.1"
                     /translation="MGQEKLYIEKELSWLSFNERVLQEAADKSNPLIERMRFLGIYSN
                     NLDEFYKVRFAELKRRIIISEEQGSNAHSRHLLGKIQSRVLKADQEFDGLYNELLLEM
                     ARNQIFLINERQLSVNQQNWLRHYFKQYLRQHITPILINPDTDLVQFLKDDYTYLAVE
                     IIRSDSIRYALLEIPSDKVPRFVNLPPEAPRRRKPMILLDNILRYCLDDIFKGFFDYD
                     ALNAYSMKMTRDAEYDLVHEMEASLMELMSSSLKQRLTAEPVRFVYQRDMPNALVEVL
                     REKLTISRYDSIVPGGRYHNFKDFINFPNVGKANLVNKPLPRLRHIWFDKAQFRNGFD
                     AIRERDVLLYYPYHTFEHVLELLRQASFDPSVLAIKINIYRVAKDSRIIDSMIHAAHN
                     GKKVTVVVELQARFDEEANIHWAKRLTEAGVHVIFSAPGLKIHAKLFLISRKENGEVV
                     RYAHIGTGNFNEKTARLYTDYSLLTADARITNEVRRVFNFIENPYRPVTFDYLMVSPQ
                     NSRRLLYEMVDREIANAQQGLPSGITLKLNNLVDKGLVDRLYAASSSGVPVNLLVRGM
                     CSLIPNLEGISDNIRAISIVDRYLEHDRVYIFENGGDKKVYLSSADWMTRNIDYRIEV
                     ATPLLDPRLKQRVLDIIDILFSDTVKARYIDKELSNRYVPRGNRRKVRAQLAIYDYIK
                     SLEQPE"
     gene            10294..11835
                     /gene="ppx"
                     /locus_tag="OO357_003189"
     CDS             10294..11835
                     /gene="ppx"
                     /locus_tag="OO357_003189"
                     /EC_number="3.6.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolyphosphatase"
                     /protein_id="HCR5888033.1"
                     /translation="MPIHDKSPRPQEFAAVDLGSNSFHMVIARVVDGAMQIIGRLKQR
                     VHLADGLGPDNMLSEEAMTRGLNCLSLFAERLQGFSPASVCIVGTHTLRQALNATDFL
                     KRAEKVIPYPIEIISGNEEARLIFMGVEHTQPEKGRKLVIDIGGGSTELVIGENFEPI
                     LVESRRMGCVSFAQLYFPGGVINKENFQRARMAAAQKLETLTWQFRIQGWNVAMGASG
                     TIKAAHEVLMEMGEKDGIITPERLEKLVKEILRHRNFASLSLPGLSEERKTVFVPGLA
                     ILCGVFDALAIRELRLSDGALREGVLYEMEGRFRHQDVRSRTASSLANQYHIDSEQAR
                     RVLDTTMQMYEQWREQQPKLAHPQLEALLRWAAMLHEVGLNINHSGLHRHSAYILQNS
                     DLPGFNQEQQLMMATLVRYHRKAIKLDDLPRFTLFKKKQFLPLIQLLRLGVLLNNQRQ
                     ATTTPPTLTLITDDSHWTLRFPHDWFSQNALVLLDLEKEQEYWEGVAGWRLKIEEEST
                     PEIAA"
     gene            complement(11874..>12981)
                     /gene="pdeF"
                     /locus_tag="OO357_003190"
     CDS             complement(11874..>12981)
                     /gene="pdeF"
                     /locus_tag="OO357_003190"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase
                     PdeF"
                     /protein_id="HCR5888034.1"
                     /translation="KQKLSHWLSPLLEPGEDVYQLSGNDLALRLNTESHQERITALDS
                     HLKQFRFFWDGMPMQPQIGVSYCYVRSPVNHIYLLLGELNTVAELSIVTNAPENMQRC
                     GAMYLQRELKDKVAMMNRLQQALEHNHFFLMAQPITGMRGDVYHEILLRMKGENDELI
                     GPGSFLPVAHEFGLSSSIDMWVIEHTLQFMAENRAKMPAHRFAINLSPTSVCQARFPV
                     EVSQLLAKYQIEAWQLIFEVTESNALTNVKQAQITLQHLQELGCQIAIDDFGTGYASY
                     ARLKNVNADLLKIDGSFIRNIVSNSLDYQIVASICHLARMKKMRVVAEYVENEEIREA
                     VLSLGIDYMQGYLIGKPQPLIDTLNEIEPIRESA"
BASE COUNT         3356 a         3368 c         3272 g         2985 t
ORIGIN      
        1 aagattattc ttgcgcgatt tgcccatcgg gccaggcgtg gatcacggct ttgatcagcg
       61 tcgccaatgg aatggcgaag aatacgcccc agaatcccca caaaccaccg aagatcacca
      121 ccgataaaat aatcaccagc ggatgcaggt taaccgcttc ggagaacaac accggtacta
      181 gcaggttgcc gtccagcgcc tgaataatca gatacaccgc gaagcagctc cagaattcag
      241 tacctgcgcc aaactggaat agcgccacgc caaccaccgg aatggtcacc acaaatgcgc
      301 caatgtacgg aataagaacc gagaagccga ccagcactgc cagcagcagc gaatagttca
      361 gcccgaagag caagaacccc agccaggtgg cgatccccac cacgatcatc tccagcactt
      421 tgccgcggat atagttggtg atttgttgat tcatctcctt ccacacctgt cctgccagcc
      481 cacggttacg cggcagcacc cggcgaacgg cgttcagcat ctgctctttg tctttcagca
      541 ggaagaagac catcaatggc accagcacca gatagacggc tatggtcagc aaaccgacca
      601 gcgaggcgag ggaaattttc accaccgaat cgcccatggt cagcatccga ctgcgcatat
      661 tttcggccat tgcatcaata atgcccgcgt ccattaacac cggatagcgg cgcggcaacg
      721 tggcggcaaa gtcagaaagc ttattgagca tccccggcat atcgcggatt aagtagatgc
      781 cctgttgcca ggcgataggc agtaccacga acgccatcag tagcaatata ccgacaaaaa
      841 ccaccaatac aatcgacgtc gcccagcggc gggagcagcc aatagattgc aggcgcacgg
      901 ttggccattc cagcaaataa gccagcacaa tagccaccag caacggagca agcaggccac
      961 taaagaaaaa gataatgcca aatccggcaa ctaaaataac cagcaaggca atcgcttccg
     1021 ggtcgctaaa acggcggcga taccattgca tcaacatttc gagcataaag accttccctg
     1081 aacctcaaga gcgggattgc gatccgcaat tgtatcgaaa tgtcacaaaa aagacttcgc
     1141 tttttatgac ggattcagga aactgaaaag tcatttgagt gggctaatct tcgccgttac
     1201 actcaaaggc ggcgaggtgg gaacgatatt tcacagtatc ggtcaaatga ctatcttcag
     1261 aaatacagga tagaggttat gttcaggcag ttgaaaaaaa acctggttgc aaccctcatt
     1321 gctgctatga ccattggtca ggtagccccg gcgtttgccg acagcgcaga caccttgccg
     1381 gatatgggaa cctccgcagg aagcacgctt tccattggtc aggaaatgca gatgggcgac
     1441 tattatgtcc gccagctacg cggcagcgcg ccgttaatta atgacccgct gttaacgcaa
     1501 tatattaatt cgctggggat gcgtctggtt tcgcatgcca attcggttaa gacaccgttt
     1561 catttttttc tgatcaacaa cgacgaaatt aacgcctttg ctttctttgg cggcaacgtg
     1621 gtgctgcact ctgccctgtt ccgttattcc gataacgaaa gtcaactggc ttcagttatg
     1681 gcgcacgaaa tctcccacgt cacccaacgt cacctggcgc gagcgatgga agatcaacaa
     1741 cgcagcgcgc cgctgacctg ggtcggcgcg ttaggttcta ttttactggc gatggccagt
     1801 ccgcaggcgg ggatggcggc gctgaccggt acactggcgg gaacgcgcca ggggatgatc
     1861 agtttcaccc agcaaaatga acaggaagcg gaccgcattg gtattcaggt gctgcaacgc
     1921 tcgggattcg atccacaggc gatgccgacc ttcctggaaa aattgctcga tcaggcgcgt
     1981 tactcctcgc gcccgccgga aattttattg actcacccgt tgccggaaag tcgtctggca
     2041 gatgcccgca accgtgctaa tcagatgcgc ccgatggtgg tgcagtcgtc ggaagatttc
     2101 tatctggcga aagcgcgcac actggggatg tataattccg gacgtaacca gctcaccagt
     2161 gatttgctgg atgaatgggc gaaaggaaac gttcgtcagc aacgagcggc gcaatatggt
     2221 cgtgctttac aggcgatgga agccaataaa tacgacgagg cgcgtaaaac gctgcaaccg
     2281 ttactggcgg cagaacctgg caacgcatgg tatctcgatc tggctaccga tatcgatctt
     2341 gggcaaaaca aagccaatga ggcaatcaat cgcctgaaaa atgcccgcga tttacgcacc
     2401 aatccggtgt tgcagctcaa cctggcgaac gcttatctgc aaggcggtca accacaagaa
     2461 gcggccaata ttctgaatcg ctacaccttt aataataaag atgacagcaa cggctgggat
     2521 ttgctggcac aggcggaagc cgcgctaaat aaccgcgatc aggagctggc tgcgcgagca
     2581 gaaggttatg cgctcgccgg acgactcgat caggccattt cgctgttgag tagcgccagt
     2641 tcgcaggtga aattaggcag cctgcaacaa gcgcgttacg atgcgcgcat cgaccagttg
     2701 cgccagctgc agaaacgctt taagccttat accaagatgt aattcgcatt aaaggaagaa
     2761 caatgaccaa acaggtaaaa atctaccata acccacgctg ttcaaagagc cgggaaacgc
     2821 tgaacctgct gaaagaaaac ggcgtggagc cagaagtggt gctctacctt gagacacccg
     2881 ccgatgcggc aacgctgcgc gatttgctga aaatgctggg gatgaatagc gcccgtgaat
     2941 tgatgcgcca gaaagaggat ctttataaag aactaaacct ggctgacagt tcgcttagcg
     3001 aagaggcgct gattcaggca atggtggata atccgaagct gatggagcgc ccgattatgg
     3061 tcgcgaatgg caaagcgcgg attggtcgtc caccggagca ggtactggaa atcgtgggtt
     3121 aagatgtgct ccatcgctag ttgaagcaca ttgccggatg cgacgcttgc cacgtcttat
     3181 ccggcctacg aatactatgg attaacttcg cgtaggtcag gcgttcgcgc cgcatccgac
     3241 aataaacacc ttatctacaa cttcagaatt tctttcacaa acggaatggt cagcttacgt
     3301 tgcgcggtaa tcgacgcacg atccaactga tccaacgtca taaatagcgt gcgcatttct
     3361 ctgtcgagcc gcttcagcaa gaaacgcccc acatcttccg gcagttcaaa accacgcaaa
     3421 cgcgcgcgta actgtagcgc ctgcaactta tcttcatcag aaagtggctg caatttgtag
     3481 atctgccccc agtcgagacg cgacgcgaga tccggtaatc ccagattcaa ctgccgcggt
     3541 ggacgatcgc cggtgatcaa caaccgtgtt ttgcccgatt ccagaattcg attgtagaga
     3601 tcgaaaatcg ccatctccca caactcatcg cctgcaatac actcaatgtt gtcgatacag
     3661 accagcgaca aatgctccat accgtcgagc acttccggaa caaaccaggt gcgtttatcc
     3721 agcgggacat agcccaccgc atcgccacgc tgcgacaatt ccgcgcaagc cgcgtgcagc
     3781 agatggctgc gccccgcgcc ttcgcgtgcc cagagataga tgtaaccgct atgttcctga
     3841 cgcagcacgt tttgcagcgc ggccagtaaa gaggagttat cccccggcca gaaacttgca
     3901 aaggtttcgt cgtcaggaag ataaagtggc aaagagagct gtgccggtgt gttcagagat
     3961 acctcaacca ggatttcaca aaatcgcgag aagtttacca cagaatccca tgatgtttga
     4021 accgggcagc aacactgccc ggtcggtaca ttatttgtct gttatatccg cgtcttctgc
     4081 gtccagcacc acttcttcag ggcgcagcac actgatcagt ttgaaaatca aacttaagcc
     4141 gataccgacg atggtcgcca gcgccatacc ttttaactcc gccgcgccga tgtttacctt
     4201 cgcgccactg acgccgatga tcaaaatcac ggaagtcagg atcaggttct gtgctttgtt
     4261 gtaatccact ttcgattcga tcaaaacacg aataccggaa gcaccaatga caccataaag
     4321 cagcagcgaa acgccgccca tcaccggcaa tgggatcatc tggatagcgg cagccagttt
     4381 accgacgcag gaaagcagga tagcgaaaat cgccgccccg ccgataaccc aggtactgta
     4441 aacacgggtg atcgccatca cgccaatgtt ttctccgtaa gtggtatttg gcgtagagcc
     4501 aaagaagccg gagatcacgg tcgacaggcc gttagcaaac atcgaacggt gcagacctgg
     4561 atcgcgcagc agatcttttt tgacgatatt agccgttact accaggtgcc ctacgtgttc
     4621 ggcaataacc actaacgccg ctggcagaat agtcagaatg gcaaaccact cgaagcgcgg
     4681 cgtatagagg gttggcagtg caaaccagtg agcattaata atcggcgtgg tatcgacaat
     4741 tcccattgcg aaagagagcg cgtaccccac cagcacgcca attaaaatcg ggataattgc
     4801 caggaaacca cgaaacagca cggaacctaa aaccgtgacc gccagggtgg taatagagat
     4861 gatgatggtt ttggagtttg gcgtttgccc ttcagccggg agtaaacccg ccataccggc
     4921 agctacgccc gccagctcca gaccgatgac ggcaacgatt gcgcccattg ccgcaggtgg
     4981 aaacagcacg tccaaccagc cggtccccgc tttcttcacg ataaaagaaa ccaggcagaa
     5041 cagcacgccg cacataataa agccgcccag cgcgacttca taccctaacg gcaacagtaa
     5101 caataccggt gaaataaagg caaagctgga accaagataa gccggaattt tccctttaca
     5161 gatgaagaga tacagcagcg ttccaatacc gttaaataac agtacagtcg ccgggttaat
     5221 atgaaataag acgggcacca ggacggttgc accaaacatg gcgaacaaat gttgcaaact
     5281 aagcgggatt gtctgtaaaa gtggcggtct ttcactcacc ccgatagcac ggcgcgtcat
     5341 agtattatcc tctgtattat gtgttatagg cgctttactc aaaaaaaagc cgactcttaa
     5401 agtcggcttt aattattttt attctttatt tcgtaccaaa gattttgtca ccggcatcgc
     5461 cgaggcccgg aataatgtat ccgtgctcgt tcagtccctg gtcaatcgat gcagtataca
     5521 gttcgacgtc cgggtgcgct ttttccagcg cagcgatacc ttctggcgca gcgaccagca
     5581 ccagaacttt gatgctgctg cagcccgctt ttttcagcag gtcgatggtc gcgataacgg
     5641 aaccaccggt tgccagcatt gggtcaacga tcagcgccat acgctcatcg atgttagaaa
     5701 ccagtttctg gaagtacggt accggctcca gcgtttcttc attacggtac ataccgacaa
     5761 cgctgatgcg cgcgctcgga acgttttcca gcacaccgtc catcatacca agacccgcac
     5821 gcagaattgg cacaacggta attttcttac ctttgatctg gtcgatttct accgggccgt
     5881 tccagccttc gatagttact ttttccgttt cgaggtcggc ggtcgcttcg taagtcagca
     5941 ggctacccac ttcggaagcg agttcgcgaa agcgcttggt gctgatatct tgctcacgca
     6001 tcagtcccag cttgtgtttg acgagtgggt gtttgacttc cacgatcttc atactctttc
     6061 tcctttgagg ggcagccaca aaaaaatcga cggattatac ctcctttctt cagggcggca
     6121 atattatttt cgttgacttt agtcaaaatg ataacagttt gagataaagt tattttatat
     6181 tcaaatggtt atgaaagaag attattccat ccgaaaacta acctttgccc tggcacaagt
     6241 cttctttcgc cgcgcgcccg gggaaaagac gtgcaaaaag gttgtgtaaa acagtctcgc
     6301 aaacgtttgc tttccctgtt agaattgcgc cgaattttat ttttctaccg caagtaacgc
     6361 gtggggaccc aagcagtgac cgataaaacc tctcttagct acaaagatgc cggtgttgat
     6421 attgacgcgg gtaatgctct ggttggaaga atcaaaggcg tagtgaagaa aacgcgtcgt
     6481 ccggaagtga tgggcggtct gggcggcttc ggtgcgctgt gtgcattgcc gcaaaaatat
     6541 cgtgaacccg tgctggtttc cggcactgac ggcgtaggta ccaagctgcg tctggcaatg
     6601 gacttaaaac gtcacgacac cattggtatt gatctggtcg ccatgtgcgt taatgacctg
     6661 gtggtgcaag gtgcggaacc gctgtttttc ctcgactatt acgcaaccgg aaaactggat
     6721 gttgataccg cttcagcggt gatcagcggc atcgcggaag gttgtctgca atcgggctgt
     6781 tctctggtgg gtggcgaaac ggcagaaatg ccggggatgt atcacggtga agattacgat
     6841 gtcgcgggtt tctgcgtggg cgtggtagaa aaatcagaaa tcatcgacgg ctctaaagtc
     6901 agcgacggcg atgtgctgat tgcactcggt tccagcggtc cgcactcgaa cggctattcg
     6961 ctggtgcgca aaattcttga agtcagcggt tgtgatccgc aaaccaccga acttgatggt
     7021 aagccattag ccgatcatct gctggcaccg acccgcattt acgtgaagtc agtgctggag
     7081 ttgattgaaa aggtcgatgt gcatgccatt gcgcacctga ccggcggcgg cttctgggaa
     7141 aacattccgc gcgtattgcc agataatacc caggcagtga ttgatgaatc ttcctggcag
     7201 tggccggaag tgttcaactg gctgcaaacg gcaggtaacg ttgagcgcca tgaaatgtat
     7261 cgcaccttca actgcggcgt cgggatgatt atcgccctgc ctgctccgga agtggacaaa
     7321 gccctcgccc tgctaaatgc caacggtgaa aacgcgtgga aaatcggtat catcaaagcc
     7381 tctgattccg aacaacgcgt ggttatcgaa taatgaatat tgtggtgctt atttccggca
     7441 acggaagtaa tttacaggca attattgacg cctgtaaaac caacaaaatt aaaggcaccg
     7501 tacgggcagt tttcagcaat aaggccgacg cgttcggcct tgaacgcgcc cgccaggcgg
     7561 gtattgcaac gcatacgctc atcgccagcg cgtttgacag tcgtgaagcc tatgaccggg
     7621 agttgattca tgaaatcgac atgtacgcac ccgatgtggt cgtgctggct ggttttatgc
     7681 gcattctcag cccggcgttt gtctcccact atgccgggcg tttgctgaac attcaccctt
     7741 ctctgctgcc gaaatatccc ggattacaca cccatcgtca ggcgctggaa aatggcgatg
     7801 aagagcacgg tacatcggtg catttcgtca ccgatgaact ggacggtggc ccggttattt
     7861 tacaggcgaa agtcccggta tttgctggtg atacggaaga tgacatcacc gcccgcgtgc
     7921 aaacccagga acacgctatt tatccactgg tgattagctg gtttgccgat ggtcgtctga
     7981 aaatgcacga aaacgccgcg tggctggatg gtcaacgtct gccgccgcag ggctacgctg
     8041 ccgacgagta atgcccccgt agttaaagcg ccagctctgc cgctggcgtt tttcaattca
     8101 cctgtaaatc gcaagctcca gcagtttttt tccccctttt ctggcatagt tggacatctg
     8161 ccaatattgc tcgccataat atccaggcag tgtcccgtga ataaaacgga gtaaaagtgg
     8221 taatgggtca ggaaaagcta tacatcgaaa aagagctcag ttggttatcg ttcaatgaac
     8281 gcgtgcttca ggaagcggcg gacaaatcta acccgctgat tgaaaggatg cgtttcctgg
     8341 ggatctattc caataacctt gatgagttct ataaagtccg cttcgctgaa ctgaagcgac
     8401 gcatcattat tagcgaagaa caaggctcca acgctcattc ccgccattta ctgggcaaaa
     8461 ttcagtcccg ggtgctgaaa gccgatcagg aattcgacgg cctctacaac gagctattgc
     8521 tggagatggc gcgcaaccag atcttcctga ttaatgaacg ccagctctcc gtcaatcaac
     8581 aaaactggct gcgtcattat tttaagcagt atctgcgtca gcacattacg ccgattttaa
     8641 tcaatcctga cactgactta gtgcagttcc tgaaagatga ttacacctat ctggcggtgg
     8701 aaattatccg tagcgatagc atccgttacg cgctgctgga gatcccatca gataaagtgc
     8761 cgcgctttgt gaatttaccg ccagaagcgc cgcgtcgacg caagccgatg attcttctgg
     8821 ataacattct gcgttactgc cttgatgata ttttcaaagg cttctttgat tatgacgcgc
     8881 tgaatgccta ttcaatgaag atgacccgcg atgccgaata cgatttagtg catgagatgg
     8941 aagccagcct gatggagttg atgtcttcca gtctcaagca gcgtttaact gctgagccgg
     9001 tgcgttttgt ttatcagcgc gatatgccca atgcgctggt tgaagtgtta cgcgaaaaac
     9061 tgactatttc ccgctacgac tccatcgtcc ccggcggtcg ttatcataat tttaaagact
     9121 ttattaattt ccccaatgtc ggcaaagcca atctggtgaa caaaccactg ccgcgtttac
     9181 gccatatttg gtttgataaa gcccagttcc gcaatggttt tgatgccatt cgcgaacgcg
     9241 atgtgttgct ctattatcct tatcacacct ttgagcatgt gctggaactg ctgcgtcagg
     9301 cttcgttcga cccaagcgta ctggcgatta aaattaacat ttaccgcgtg gcgaaagatt
     9361 cacgcatcat cgactcgatg atccacgccg cacataacgg taagaaagtc actgtggtgg
     9421 ttgagttaca ggcgcgtttc gacgaagaag ccaacattca ctgggcgaag cgcctgaccg
     9481 aagcaggcgt gcacgttatc ttctctgcgc cggggctgaa aattcacgcc aaactgttcc
     9541 tgatttcacg taaagaaaac ggtgaagtgg tccgttacgc acacatcggg accgggaact
     9601 ttaacgaaaa aaccgcgcgt ctttatactg actattcgtt gctgaccgca gatgcgcgta
     9661 tcaccaacga agtacggcgg gtatttaact ttattgaaaa cccataccgc ccggtgacat
     9721 ttgattattt aatggtgtca ccgcaaaact ctcgccgtct gttatatgaa atggtagacc
     9781 gcgaaatcgc caacgcgcag caagggctgc ccagtggtat caccctgaag ctaaataacc
     9841 ttgtcgataa aggcctggtt gatcgtctgt atgcggcctc cagctccggc gtaccggtta
     9901 atctgctggt tcgcggaatg tgttcgctga tccccaatct ggaaggcatt agcgacaaca
     9961 ttcgtgccat cagtattgtt gaccgttacc ttgaacatga ccgggtttat atttttgaaa
    10021 atggcggcga taaaaaggtc tacctttctt ccgccgactg gatgacgcgc aatattgatt
    10081 atcgtattga agtggcgaca ccgctgctcg atccgcgcct gaagcagcgg gtgctggaca
    10141 tcatcgacat attgttcagc gatacggtca aagcacgtta tatcgataaa gaactcagta
    10201 atcgctacgt tccccgcggc aatcgccgca aagtacgggc gcagttggcg atttacgact
    10261 acatcaaatc actcgaacaa cctgaataac cctatgccaa tacacgataa atcccctcgt
    10321 ccgcaggagt ttgctgcggt cgatcttggt tcaaacagtt ttcacatggt catagcccgt
    10381 gtggtagatg gtgccatgca gattattggc cgcctgaaac agcgggtgca tctggcggac
    10441 ggcctggggc cagataatat gttgagtgaa gaggcaatga cgcgcggttt gaactgtctg
    10501 tcgctgtttg ccgaacggct acaagggttt tctcctgcca gcgtctgtat agtcggcacc
    10561 catacgctgc gtcaggcgct gaacgccact gactttctga aacgcgcgga aaaggtcatt
    10621 ccctacccga ttgaaattat ttccggtaat gaagaagccc gtctgatttt tatgggcgtg
    10681 gaacataccc aaccggaaaa aggtcgcaaa ctggttattg atattggcgg cggatctacg
    10741 gaactggtga ttggtgaaaa tttcgaacct attctcgttg aaagccgccg gatgggttgt
    10801 gtcagctttg cccagcttta ttttcctggc ggggtcatca ataaagagaa ttttcagcgc
    10861 gctcgcatgg cggcagcaca aaaactggaa actttaacct ggcaattccg tattcagggc
    10921 tggaacgttg caatgggcgc ttccggtacc ataaaagccg cccatgaagt gttaatggaa
    10981 atgggcgaga aagacgggat aattaccccg gaacgtctgg aaaaactggt aaaagaaatt
    11041 ttacgtcacc gtaatttcgc atcgctgagt ttaccgggtc tttccgaaga gcggaaaaca
    11101 gtcttcgttc ctggactggc gattttatgt ggtgtgtttg atgctttagc catccgtgaa
    11161 ctgcgccttt ctgacggggc gttacgcgaa ggcgtactgt atgaaatgga aggacgtttc
    11221 cgtcatcagg atgtgcgtag tcgcaccgcc agcagcctcg ccaaccagta tcacatagac
    11281 agcgaacagg cccgacgagt gctggatacc actatgcaaa tgtacgaaca gtggcgggaa
    11341 cagcaaccga agctggcgca tccgcaactg gaggcgctac tgcgatgggc cgccatgctg
    11401 catgaggtcg ggttgaatat caaccacagc ggtttgcatc gccactccgc ttatattctg
    11461 caaaacagtg acttgccggg ttttaatcag gaacagcagc tgatgatggc gacactggtg
    11521 cgctatcacc gtaaagcgat taagctcgac gatctgccgc gctttacctt gtttaagaag
    11581 aaacagttcc tgccactgat acagctattg cgccttggcg tattactaaa caatcaacgt
    11641 caggcaacca ccacaccgcc aacattgaca ctgattactg atgacagtca ctggacactg
    11701 cgtttcccgc atgactggtt tagtcagaat gcgctggtac tgcttgatct ggaaaaggag
    11761 caagaatact gggaaggcgt ggctggctgg cggttgaaaa ttgaagaaga aagtacccca
    11821 gaaatcgccg cttaatgctt tgcgggccga cgagaaatgt cggcccgcat tattcaggca
    11881 ctttcgcgaa tgggttcgat ttcattcagc gtatcaatta acggttgcgg cttaccaata
    11941 agataaccct gcatataatc gatccccaaa gagagcaccg cctcgcggat ctcttcgttt
    12001 tcaacgtact ctgccactac ccgcattttc ttcattcgcg ccagatggca aatcgacgcc
    12061 acaatctgat aatccagact atttgacaca atattgcgga taaagctgcc gtcaatttta
    12121 agcagatcgg cattcacatt tttaagccgt gcatagctgg cgtagccggt gccgaaatca
    12181 tcaatcgcaa tctggcagcc taattcctga agatgctgca aggtgatttg cgcctgctta
    12241 acattggtca gagcattact ttcggtgact tcaaaaataa gttgccacgc ttcaatctga
    12301 tatttagcca gcaactgact gacttcaaca ggaaaacgag cctgacatac cgaggttgga
    12361 gacagattaa tagcaaaacg gtgagcgggc atcttcgctc tgttttcagc cataaattgc
    12421 agcgtatgct caatgaccca catgtcgata ctcgacgata aaccaaattc gtgcgcgacc
    12481 ggcaagaagc taccggggcc gatcagttca tcattctcac ctttcatgcg cagaagaatt
    12541 tcatggtaaa catcaccacg cataccggta atcggctggg ccatcaggaa aaaatggttg
    12601 tgttccagcg cctgctgtag tcgattcatc atcgcgactt tatctttcaa ttcgcgttgc
    12661 aaatacattg ccccgcaacg ctgcatattt tccggggcgt tggtcacgat ggaaagttcg
    12721 gcgaccgtat ttagctctcc cagcagcagg tagatatgat tcactggcga gcgcacatag
    12781 cagtaactga cgccaatctg cggttgcatc ggcatcccat cccaaaagaa acgaaattgc
    12841 ttgagatgac tatccagtgc ggtaatgcgc tcctggtgcg attctgtatt cagtcgcagc
    12901 gcgagatcgt tacccgaaag ctgataaaca tcttcacccg gttccagcaa gggtgacagc
    12961 cagtgagaaa gtttttgctt g
//
LOCUS       DAJTRC010000099        12759 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.099, whole genome shotgun sequence.
ACCESSION   DAJTRC010000099 DAJTRC010000000
VERSION     DAJTRC010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12759)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12759)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12759
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.099"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(270..989)
                     /locus_tag="OO357_003191"
                     /pseudo
     CDS             complement(270..989)
                     /locus_tag="OO357_003191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
     gene            complement(1005..1337)
                     /gene="cusF"
                     /locus_tag="OO357_003192"
     CDS             complement(1005..1337)
                     /gene="cusF"
                     /locus_tag="OO357_003192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     metallochaperone CusF"
                     /protein_id="HCR5888035.1"
                     /translation="MKKALQVAMFSLFTVIGFNAQANEHHHETMSEAQPQVISATGVV
                     KGVDLESKKITIHHDPIAAVNWPEMTMRFTITPQTKMSEIKTGDKVAFNFVQQGNLSL
                     LQDIKVSQ"
     gene            complement(1361..2743)
                     /gene="cusC"
                     /locus_tag="OO357_003193"
     CDS             complement(1361..2743)
                     /gene="cusC"
                     /locus_tag="OO357_003193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter outer
                     membrane channel CusC"
                     /protein_id="HCR5888036.1"
                     /translation="MSPCKLLPFCVALALTGCSLAPDYQRPVMPVPQQFSLSQNGLVN
                     AADNYQNVGWRTFFVDNQVKTLISEALVNNRDLRMATLKVQEARAQYRLTDADRYPQL
                     NGEGSGSWSGNLKGDSATTREFSTGLNASFDLDFFGRLKNMSEAERQNYLATEEAQRA
                     VHILLVSNVAQSYFNQQLAYAQLQIAEETLRNYQQSYAFVEKQLLTGSSNVLALEQAR
                     GVIESTRSDIAKRQGELAQANNALQLLLGSYGKLPQAQTVNSGSLQSVKLPAGLSSQI
                     LLQRPDIMEAEHALMAANANIGAARAAFFPSISLTSGISTASSDLSSLFNASSGMWNF
                     IPKIEIPIFNAGRNQANLDIAEIRQQQSVVNYEQKIQNAFKEVADALALRQSLDDQIS
                     AQQRYLASLQITLQRARALYQHGAVSYLEVLDAERSLFATRQTLLDLNYARQVNEIYL
                     YTALGGGWQQ"
     gene            2900..3583
                     /gene="cusR"
                     /locus_tag="OO357_003194"
     CDS             2900..3583
                     /gene="cusR"
                     /locus_tag="OO357_003194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper response regulator transcription factor
                     CusR"
                     /protein_id="HCR5888037.1"
                     /translation="MKLLIVEDEKKTGEYLTKGLTEAGFVVDLADNGLNGYHLAMTGD
                     YDLIILDIMLPDVNGWDIVRMLRSANKGMPILLLTALGTIEHRVKGLELGADDYLVKP
                     FAFAELLARVRTLLRRGAAVIIESQFQVADLMVDLVSRKVTRSGTRITLTSKEFTLLE
                     FFLRHQGEVLPRSLIASQVWDMNFDSDTNAIDVAVKLLRGKIDNDFEPKLIQTVRGVG
                     YMLEVPDGQ"
     gene            3573..5015
                     /gene="cusS"
                     /locus_tag="OO357_003195"
     CDS             3573..5015
                     /gene="cusS"
                     /locus_tag="OO357_003195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) sensor histidine kinase CusS"
                     /protein_id="HCR5888038.1"
                     /translation="MVSKPFQRPFSLATRLTFFISQATIAAFFAFAWIIIHSVKVHFA
                     EQDINDLKEISATLERVLNHPDETQARRLMTLEDIVSGYSNVLISLADSHGKTVYHSP
                     GAPDIREFARDAIPDKDARGGEVYLLSGPTIMMPGHGHGHMEHSNWRMINLPVGPLVD
                     GKPIYTLYIALSIDFHLHYINDLMNKLIMTASVISILIVFIVLLAVHKGHAPIRSVSR
                     QIQNITSKDLDVRLDPQTVPIELEQLVLSFNHMIERIEDVFTRQSNFSADIAHEIRTP
                     ITNLITQTEIALSQSRSQKELEDVLYSNLEELTRMAKMVSDMLFLAQADNNQLIPEKK
                     MLNLADEVGKVFDFFEALAEDRGVELRFVGDKRQVAGDPLMLRRALSNLLSNALRYTP
                     TRETIVVRCQTVDHQVQVSVENPGTPIAPEHLPRLFDRFYRVDPSRQRKGEGSGIGLA
                     IVKSIVVAHKGTVAVTSDARGTRFVITLPA"
     gene            5165..7402
                     /gene="nrfB"
                     /locus_tag="OO357_003196"
     CDS             5165..7402
                     /gene="nrfB"
                     /locus_tag="OO357_003196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage adsorption protein NrfB"
                     /protein_id="HCR5888039.1"
                     /translation="MDWLLDVFATWLYGLKVIAITLAVIMFISGLDDFFIDVVYWVRR
                     IKRKLSVYRRYPRMSYRELYKPDEKPLAIMVPAWNETGVIGNMAELAATTLDYENYHI
                     FVGTYPNDPDTQRDVDEVCARFPNVHKVVCARPGPTSKADCLNNVLDAITQFERSANF
                     AFAGFILHDAEDVISPMELRLFNYLVERKDLIQIPVYPFEREWTHFTSMTYIDEFSEL
                     HGKDVPVREALAGQVPSAGVGTCFSRRAVTALLADGNGIAFDVQSLTEDYDIGFRLKE
                     KGMTEIFVRFPVVDEAKEREQRKFLQHARTSNMICVREYFPDTFSTAVRQKSRWIIGI
                     VFQGFKTHKWTSSLTLNYFLWRDRKGAISNFVSFLAMLVMIQLLLLLAYESLWPDAWH
                     FLSIFSGSAWLMTLLWLNFGLMVNRIVQRVIFVTGYYGLTQGLLSVLRLFWGNLINFM
                     ANWRVLKQVLQHGDPRRVAWDKTTHDFPSVTGDTRSLRPLGQILLENQVITEEQLDTA
                     LRNRVEGLRLGGSMLMQGLISAEQLAQALAEQNGVAWESIDAWQIPSSLIAEMPASVA
                     LHYAVLPLRLENDELIVGSEDGIDPVSLAALTRKVGRKVRYVIVLRGQIVTGLRHWYA
                     RRRGHDPRAMLYNAVQHQWLTEQQTGEIWRQYVPHQFLFAEILTTLGHINRSAINVLL
                     LRHERSSLPLGKFLVTEGVISQETLDRVLTIQRELQVSMQSLLLKAGLNTEQVAQLES
                     ENEGE"
     gene            7389..10357
                     /gene="nfrA"
                     /locus_tag="OO357_003197"
                     /pseudo
     CDS             7389..10357
                     /gene="nfrA"
                     /locus_tag="OO357_003197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415100.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriophage adsorption protein NfrA"
     gene            10358..11248
                     /locus_tag="OO357_003198"
     CDS             10358..11248
                     /locus_tag="OO357_003198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4434 family protein"
                     /protein_id="HCR5888040.1"
                     /translation="MRKFIFVLLTLLLVSPFSFAMKGIIWQPQNRDSQVTDTQWQGLM
                     SQLRFQGFDTLVLQWTRYGDAFTQPEQRTLLFKRAAAAQQTGLKLIVGLNADPEFFMH
                     QKQSSAALESYLNRLLAADLQQARLWSAAPGITPDGWYISAEIDDLNWRSEAARQPLL
                     TWLNNAQRLISDVSAKPVYISSFFAGNMSPDGYRQLLEQVKATGVNVWVQDGSGVDKL
                     TAEQRERYLQASADCQSSAPASGIVYELFVAGKGKTFTAKPKPDAEIASLLAKRSSCG
                     KDMLYFSLRYLPVAHGILEY"
     gene            11431..12192
                     /gene="envY"
                     /locus_tag="OO357_003199"
     CDS             11431..12192
                     /gene="envY"
                     /locus_tag="OO357_003199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator EnvY"
                     /protein_id="HCR5888041.1"
                     /translation="MQLSSSEPCVVILTEKEVEVSVNNHATFTLPKNYLAAFACNNNV
                     IELSTLNHVLITHINRNIINDYLLFLNKKLTCVKPWSRLATPVIACHSRTPEVFRLAT
                     NHSKQQSSKPCEAELTRALLFTVLSNFLEQSRFIALLMYILRSSVRDSVCRIIQSDIQ
                     HYWNLRIVASSLCLSPSLLKKKLKNENTSYSQIVTECRMRYAVQMLLMDNKNITQVAQ
                     LCGYSSTSYFISVFKAFYSLTPLNYLAKQRQKVMW"
     gene            complement(12238..12314)
                     /locus_tag="OO357_003200"
     tRNA            complement(12238..12314)
                     /locus_tag="OO357_003200"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12278..12280),aa:Arg,seq:tct)
     gene            12557..>12759
                     /locus_tag="OO357_003201"
     CDS             12557..>12759
                     /locus_tag="OO357_003201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HCR5888042.1"
                     /translation="MKPTSVIIMDTHPIIRMSIEVLLQKNSELQIVLKTDDYRITIDY
                     LRTRPVDLIIMDIDLPGTDGFTFL"
BASE COUNT         3122 a         3309 c         3237 g         3091 t
ORIGIN      
        1 attcttgtgg gccagaatat ggtttaccat aaatagtacg cagttttgtg tcgcttatag
       61 ccataaataa tatttggtac gctcagaaat aatggtttgg tacacatctg gtacacaata
      121 tcacatggac aacagcgcac agtaaacaac tatatttgac gttcgtagac atactcaggt
      181 gaaaaaaagg ttgtttttcg agttataaca gacagttaat taactacagt ggacaatgct
      241 agacaatgac agacacagat aagcaaccta ccttcctctt tcgcgaccac gttatctttg
      301 gcgatattca ttcccgcgcg cagatcaaac gcggtgatca cgccatcaat gggcgctttg
      361 agcgtaaagc gagtctggat tttttgcgtg gcgatcaggt ggcgaatatc cgcctccggc
      421 attcccgcca gtcgcagccg ctcaagaatg ccttcagtct gggtcgccgt accgccggtt
      481 tcgcgcaaca gtaaatactc actctgcgct tccacccagt cgggaatggt caggtcgaga
      541 agcggtgtgc ccttttgtac tttatcgccc acggtaagcg gatacacctt gtcgataaac
      601 ccggcagcgc gagcctgcac aatggcatac tgatactcgt tgtaactgac attcgtcggg
      661 aaactctggg caaaagtcag cggcccgcgc gtgacggtag ccgttttcac ccccagattc
      721 tgcgtctgag tcgggtcaat gcgcacacca gacgcagaac tctcttcatc ggcatatttc
      781 ggcaccagat ccatatccat aaacggcgat ttacctggtt tatcgaaccg cgtattggga
      841 tacatcgggt cgtaccagaa taagatttta cgttctgcgg tcggcatttt ttctgcgtgc
      901 ggttccgcct ggtcaaacca ggtaaaacct gccgctgaaa taataccgcc cgcgatcatg
      961 ctgccgataa taagcgcgat ttttttcatc tcattaaacc tgggttactg gctgacttta
     1021 atatcctgta ataaagaaag gttgccctgc tggacaaaat taaacgccac tttgtcgccg
     1081 gttttaattt cactcatttt cgtctgcggg gtgatggtaa agcgcatggt catctccggc
     1141 cagttgacgg cagcaatcgg atcgtgatgg atggtgattt ttttgctttc cagatcaaca
     1201 ccttttacca caccggtggc actaatgacc tgtggttgtg cttcgctcat ggtttcatga
     1261 tgatgttcgt tagcctgggc attaaagcca ataacggtaa acagactgaa cattgcgact
     1321 tgcagtgctt ttttcattta ttctctcctg gagttaaaaa ttattgctgc caaccgcccc
     1381 ctaaggcggt atacaaataa atttcgttaa cctgacgggc ataattcagg tcaagtaaag
     1441 tttgtcgggt tgcaaataaa gaacgctcgg catccagcac ttccagataa cttaccgcgc
     1501 cgtgctgata taatgcccgc gcccgttgca gggtaatttg cagcgacgcc agataacgct
     1561 gctgggcgct gatttgatcg tccaggcttt gacgtaatgc cagcgcatct gccacctctt
     1621 taaaggcgtt ctggattttc tgttcataat tcaccaccga ctgctgctgg cgaatttcgg
     1681 cgatatccag attggcctgg ttgcgtccgg cattaaaaat ggggatctca attttaggaa
     1741 taaaattcca catcccgctg ctagcgttaa ataatgacga cagatcgctg ctggcggttg
     1801 atattccgct ggtcaggctg atagacggga aaaatgccgc acgcgcagca ccaatattag
     1861 cattagccgc cattaacgcg tgttcagctt ccataatatc cgggcgctgc aataagattt
     1921 gcgacgacaa gcccgccggt aatttaacgc tttgcaggct gccgctgttt actgtctgcg
     1981 cttgcggtag cttgccgtag cttcccaata acagttgcaa tgcattattc gcctgcgcca
     2041 gttccccctg acgtttagcg atgtcactgc gggtactttc tatcaccccg cgagcctgtt
     2101 ccagcgccag aacattgctg ctaccggtca acagttgttt ttcgacaaac gcatatgact
     2161 gctgataatt acgcagcgtt tcttcggcta tttgcaattg cgcatacgcc agctgctgat
     2221 tgaaatagct ttgcgcgaca ttagaaacca gcaaaatatg caccgcgcgc tgggcttcct
     2281 cagtggctaa ataattttgg cgctcggctt cgctcatgtt ctttaagcga ccgaaaaaat
     2341 cgagatcaaa gctggcgtta aggccagtcg agaactcccg cgtcgtggct gaatcgcctt
     2401 taagattgcc gctccagctg ccgctgccct cgccattgag ctgtgggtag cggtcggcat
     2461 cggtcagacg atattgcgcc cgtgcctcct gcactttcag cgtcgccatg cgcaaatccc
     2521 ggttattcac cagcgcctcg ctaatcagcg tcttcacctg attatcaaca aaaaaggtgc
     2581 gccagcccac gttctggtag ttatccgctg cgttaaccag gccgttctgg ctgagtgaga
     2641 actgctgcgg tacgggcatt accggacgct gataatccgg tgccagtgaa caaccagtta
     2701 gcgcaagggc cacacaaaat ggcagaagtt tacaaggaga cataggctca taatttctgg
     2761 tgattttatg tcgccaactt tactcgccag gctctgattt tccggtgaca ggaaaatgac
     2821 aaaattgtca ttttgccaat aagcgattgc catctgatcc cgctactcta gaattgcccg
     2881 ggcaacatgc ggaggaaata tgaaactgtt gattgtcgaa gatgaaaaga aaaccggaga
     2941 atacttgacc aaagggttaa ccgaagccgg ttttgtggtc gatttggccg acaacgggct
     3001 gaatggctac catctggcga tgaccggtga ttatgacctg ataatcctcg atattatgct
     3061 gccagacgtg aacggctggg atatcgtgcg catgttgcgc tccgccaata aagggatgcc
     3121 gattctgttg cttaccgcgc ttggcaccat tgaacatcgc gtcaaggggc tggagttggg
     3181 ggcagatgac tacctggtga aaccgttcgc ttttgctgaa ctgctggcgc gggtgcgcac
     3241 tctcctgcgg cgcggggcgg cggtgattat cgaaagtcag tttcaggttg ccgatctgat
     3301 ggtcgatctc gtcagccgca aagtcacccg cagcggcacg cgcatcactc tgaccagtaa
     3361 agaatttact ctgctggagt tttttctccg ccatcagggc gaagtgctgc cccgctcgct
     3421 tatcgcctcg caggtatggg acatgaattt cgacagcgac actaacgcca ttgatgtggc
     3481 ggtgaagctg ctacgcggca aaatcgacaa cgactttgag ccgaagctga ttcaaaccgt
     3541 gcgcggcgtg ggttacatgc ttgaggtgcc ggatggtcag taagccattt cagcgcccgt
     3601 tttcgctggc aacccgcctg acctttttta tcagccaggc caccatcgcg gcgtttttcg
     3661 cctttgcatg gatcataatc cactcagtaa aagtgcattt tgccgagcag gacattaatg
     3721 atttaaaaga gattagcgcc acccttgaac gggtactaaa tcaccctgac gaaacgcaag
     3781 cccgacgctt aatgacgctg gaagatatcg tcagtggtta ttccaacgtg ttgatttccc
     3841 tggcagatag ccacggcaaa acggtgtatc actcccccgg tgcgcctgat atccgcgagt
     3901 ttgcacgtga tgccataccc gataaagacg cgcggggtgg cgaggtgtat ctcctttccg
     3961 gcccgacgat tatgatgcca ggccacggtc acgggcatat ggaacacagc aactggcgga
     4021 tgattaactt gccggttggc ccgttggtgg acggcaaacc gatttatacg ctctacatcg
     4081 cgctgtcgat cgattttcat cttcattaca taaatgattt gatgaataaa cttattatga
     4141 ccgcatcggt aatcagcatc ctgatcgtct ttatcgtgct gttggcggta cataaaggtc
     4201 acgcgccgat ccgcagcgtc agccgtcaaa tccagaatat cacctcgaaa gatctcgacg
     4261 ttcgccttga cccgcagacc gtgccgattg agctggaaca gctggtactg tcgttcaacc
     4321 atatgatcga gcgtattgag gatgtcttta ctcgccagtc caatttctca gcggatatcg
     4381 cccacgaaat tcgcacacca attactaatc tcattacgca aacggaaatc gccctcagcc
     4441 agtcgcgcag ccagaaggag ctggaagatg tgctctactc taatctcgaa gagctgacgc
     4501 gaatggcgaa aatggtcagc gatatgctgt ttctcgctca ggccgataac aaccagctaa
     4561 tccccgaaaa gaaaatgctc aacctggcgg atgaagtcgg caaagtgttc gattttttcg
     4621 aggcgctagc ggaagatcgc ggcgtggagt tgcggtttgt tggcgacaaa cgtcaggttg
     4681 cgggcgatcc gttgatgctg cgtcgggcgc taagcaacct gctttctaat gcccttcgtt
     4741 atacgccaac cagagagaca attgtagtac gctgtcagac ggtcgatcac caggtgcaag
     4801 tttccgtcga aaaccccggt acgcccattg cgcccgagca cttaccgcga ttgtttgacc
     4861 gtttctatcg cgttgacccc tcccgccagc gaaaaggtga aggtagcggt attgggctgg
     4921 cgatagtgaa atcgattgtt gtcgcgcata aaggcacggt tgcggtaacg tcagatgcgc
     4981 gggggacaag gtttgttatc acattacccg cttaattatt cagcaaacgg caacttttat
     5041 aaccagtgta aaaataacgt gccgcaatag tcatacatat taatggtaaa aagctgtcac
     5101 aattcataaa aaaccttaat atacgccacc ctaaacataa ccagcgttaa tgtaaggttt
     5161 ttgtgtggac tggcttcttg atgtttttgc tacctggctc tacggcttaa aagtaatcgc
     5221 gataacgtta gcggtcatca tgttcatcag cgggctggac gattttttta ttgatgtcgt
     5281 ctactgggta cgccgcatta aacgcaagtt gagtgtttat cgccgctacc cgcgaatgag
     5341 ttaccgcgaa ctgtataaac cagatgaaaa accgttagcg attatggttc cggcgtggaa
     5401 tgaaacgggc gtcatcggca atatggccga gctggcggcg accacgctcg actacgaaaa
     5461 ctatcatatc tttgttggca cctaccccaa cgaccccgat actcaacgtg atgttgacga
     5521 agtgtgcgct cgctttccga acgtgcataa ggtagtctgc gcgcgtcctg gtcccaccag
     5581 caaagccgac tgcctgaaca acgtgctgga cgccatcacc cagtttgaac gtagcgccaa
     5641 tttcgctttt gctggtttta ttctgcatga cgccgaagat gtgatttcac cgatggaatt
     5701 gcgtctgttc aactatctgg tcgagcgtaa agatctgatt cagatcccag tgtatccgtt
     5761 tgaacgcgaa tggacgcact tcaccagcat gacttacatt gatgagtttt cagaactgca
     5821 tggcaaagat gttccggtgc gtgaagccct cgccggacaa gtgcccagcg caggcgtcgg
     5881 cacctgtttc agccgccgcg ccgtgaccgc actgttagct gacggtaacg gtattgcttt
     5941 cgacgtgcag agtcttactg aagattacga cattggcttc cgcctgaaag aaaaaggtat
     6001 gacggaaatt tttgtccgtt ttccggtggt ggacgaagcc aaagaacgcg agcagcgtaa
     6061 atttttacag cacgcacgga cgtcaaacat gatctgcgtg cgcgaatatt tccccgatac
     6121 cttttcgact gcggttcgac aaaaatctcg ctggatcatc ggcattgttt tccagggctt
     6181 taaaacccat aaatggacct ccagcctgac gctgaactac tttctctggc gcgaccgcaa
     6241 aggggcaatc agtaactttg tcagcttcct cgcgatgctg gtgatgatcc agcttttgct
     6301 gttgctggcg tatgaaagtt tgtggcccga tgcctggcat ttcctttcta tttttagcgg
     6361 cagcgcatgg ttaatgaccc tgctgtggct aaactttggc ttgatggtta accgcatcgt
     6421 gcagcgagtg attttcgtca ctggctacta cggcctcacg cagggcctgc tttccgtcct
     6481 gcgtcttttc tggggcaacc tgattaactt tatggctaac tggcgcgtgc tgaaacaggt
     6541 tcttcagcac ggcgatccgc gtcgcgtcgc gtgggataaa acaacgcatg acttccccag
     6601 cgtcactggc gatacccgct cgttgcgccc gttaggtcaa attctgctgg aaaatcaggt
     6661 catcactgaa gaacaactcg atacagcact gcgtaatcgc gtcgaaggtc tacgcctggg
     6721 cggttcaatg ctgatgcagg ggctgattag cgccgagcag ctggcacagg cgctggcaga
     6781 gcaaaacggc gtggcgtggg aatccatcga tgcctggcag atcccgtcct cgctgattgc
     6841 cgaaatgcca gcctccgtag cgctgcatta tgcagtactg ccgctgcgtc tggaaaatga
     6901 tgagttaatt gtcggcagtg aagatggtat tgacccggtt tcgctggcgg ccctgacgcg
     6961 taaagtcgga cgcaaagtgc gttacgtcat tgttctgcgg ggacaaattg tcacagggtt
     7021 acgccactgg tatgcacgcc gacgcggtca cgatccgcgg gcaatgttgt acaatgcggt
     7081 tcagcatcag tggctcacgg aacagcagac cggtgaaatc tggcggcaat atgtgccgca
     7141 tcagttcctg ttcgccgaaa tactgaccac gctcggtcat attaatcgtt cagcaattaa
     7201 cgtgttgtta ttgcgccatg aacgcagttc tctgccgctc ggcaagtttt tggtcaccga
     7261 aggcgttatc agccaggaaa cgttggatcg cgtcctgaca attcaacgcg aattacaagt
     7321 ttcgatgcaa tcactattac tcaaagcagg tttaaacaca gaacaggttg cgcaactgga
     7381 gtccgaaaat gaaggagaat aaccttaatc gcgtcatcgg atggtctggt ttactgctga
     7441 cgtctttatt gagtaccagc gcactcgcag acaatatcgg caccagcgca gaagagctgg
     7501 ggctgagcga ttatcgccat tttgttattt atccccgtct cgataaggcg ctgaaggcac
     7561 agaaaaataa cgacgaagca accgccatcc gcgaatttga atatatacac caacaggtgc
     7621 cggataatat tccgctgact ttataccttg cggaagccta tcgtcatttt ggtcatgatg
     7681 accgggcgcg gctgttgctt gaggatcaac tgaaacgtca cccaggagat gcccgacttg
     7741 agcgcagtct ggcggctatt ccagttgaag tgaaaagcgt tacgactgtt gaagaactgc
     7801 ttgcccagca aaaagcgggc gacgctgcgc cgaccctgcg ttgtcgcagt gaagtcgggc
     7861 agaatgccct gcggctggca caattacctg tcgccagagc gcaactgaac gatgcgacgt
     7921 ttgctgcatc gccggaagga aaaacgctgc gaaccgatct gctgcaacgg gcaatctacc
     7981 tgaaacaatg atcccaggca gatacgctat acaatgaagc acgccagcag aacacattaa
     8041 gcgcggcaga acgccgtcag tggtttgacg tgcttcttgc cgggcagctg gacgatcgga
     8101 tcctggcact gcaatcacag gggatcttca ccgatcctca gtcatatatt acttacgcga
     8161 ccgcgctggc ttatcgtggc gaaaaagcac gcctccagca ttatctcatt gaaaataagc
     8221 cgctgtttac cacgggcgca caagagaaaa gttggctcta tctgttatct aaatacagcg
     8281 ccaaccccgt tcaggcattg gcgaattata cggtgcagtt tgccgataac cgccagtatg
     8341 ttgttggcgc gacgctaccg gtgctgttaa aagaaggtca gtacgacgca gcgcaaaaac
     8401 tgctcgccac cctccccgcc aatgaaatgc ttgaggagcg ttatgctgtc agcgtggcga
     8461 cccgtaacaa ggctgaatct ctgcgtctgg cacgattgct gtatcagcaa gaaccgacaa
     8521 atcttccccg cctggatcaa ctgacctggc aactgatgca gaacgagcag tcacgcgaag
     8581 ctgccgattt attgctgcaa cgctatcctt tccagggcga tgcgcgtgtc agccagactt
     8641 taatggcgcg actggcgtct ctgctggaaa gtcatcctta cctagcaacg ccggcgaagg
     8701 tggcgatttt atcgaaaccc ttaccgctgg cggagcaacg tcagtggcaa agtcagttgc
     8761 cgggtattgc agataattgc ccggcaatag ttcgcttgct gggcgatatg tcgccttcct
     8821 acgatgctgc cgcctggaac cgtctggcaa agtgttatcg ggacacgcta cccggtgtgg
     8881 cgttgtatgc atggcttcag gccgaacaac gacaaccgag cgcctggcaa catcgtgcgg
     8941 tagcctatca ggcgtatcag gttgaggact acgccaccgc actggcggcc tggcagaaaa
     9001 tcagtcttca cgacatgagc aatgatgatc tgctcgccgc cgccaacacc gctcaggcag
     9061 cgggaaatgg cgcagctcgc gatcgctggc ttcaacaggc agaacaacgt gagctgggaa
     9121 acaatgctca atactggtgg ctgcatgcgc gacgttatat cccagctcag ccagaactcg
     9181 cactgaacga tctcacgcgc tcaatcaata ttgccccttc tgccaactct tacgttgcgc
     9241 gggcgacaat ttatcgccaa cgtcataatg ttccggccgc ggtgagtgat ttgcgcgccg
     9301 cgctggaact ggaaccaaat aatagcaaca cccaggcagc gcttggttac gccttgtggg
     9361 atagcggtga tatcgcacag tcgcgggaaa tgctcgaaca ggcgcataaa gggcttccgg
     9421 acgatccggc actgatccga caactggcct acgtgaacca gcgtctggat gacatgcctg
     9481 cgacgcagca ctacgcccgg ctcgtgattg atgacattga taatcaggca taaccccact
     9541 gaccccagag caaaatcagc aacgcttcaa tttccgccgt ttgcatgagg aggtcggtcg
     9601 ccgctggacg ttcagtttcg attcttccat cggcttgcgt tccggcgcaa tgagtaccgc
     9661 taacaataat gtcggcggtg cagcgcctgg gaaaagctat cgtagctacg gacaactgga
     9721 agccgagtac cgcatcggac gcaatatgct gctggaaggc gacctgctct cagtttatag
     9781 ccgcgtcttt gccgataccg gagaaaacgg ggtgatgatg ccggtgaaaa atccgatgtc
     9841 cggcaccggt ctgcgctgga agccgctgcg cgatcagatc tttttcctcg ccgtcgaaca
     9901 gcagttgccg ctgaacgggc aaaatggcgc atccgatacc atgctgcgcg ccagcgcctc
     9961 attctttaat ggcggcaaat acagcgacga atggcacccg aacggttcag gctggtttgc
    10021 ccaaaacctg tacctcgatg cggcgcaata tatccgccag gatattcagg cgtggacggc
    10081 agattatcgc gtcagctggc atcagaaggt agctaacgga cagactattg agccttacgc
    10141 tcacgttcag gacaacggct atcgtgataa aggcactcag ggcgcgcagc ttggcggtgt
    10201 cggggtccgc tggaatattt ggaccggcga gacgcactac gacgcctggc cgcacaaagt
    10261 cagtctcggc gtcgagtatc aacatacctt taaggcgatt aatcaacgta acggagagcg
    10321 caacaacgcg tttctcacca ttggagtgca ctggtaaatg cgtaagttca ttttcgtatt
    10381 gctgacactg cttttggtca gccctttttc ctttgcgatg aaaggtatta tctggcaacc
    10441 acaaaaccga gatagtcagg ttaccgatac ccagtggcag gggctgatga gtcagttacg
    10501 ttttcaaggc ttcgataccc tggttttgca atggacccgt tacggcgatg catttaccca
    10561 gccagaacag cgcacgttat tgtttaagcg tgccgcagct gcgcaacaga ctggcctgaa
    10621 gctgattgtc gggctgaacg ccgatccgga attttttatg caccagaaac agtcgtctgc
    10681 agcgctggaa agctatctta atcgcctgct ggctgccgat ctccagcaag ccagattatg
    10741 gagcgccgcg cctggcataa cgccggatgg ctggtacatc agcgcggaaa ttgacgacct
    10801 gaactggcgc agcgaagccg cccgtcaacc tttgctaaca tggttaaaca acgcgcagcg
    10861 gctgattagc gatgtttcag caaaaccggt ttatatcagt agttttttcg ccggaaacat
    10921 gtcgcccgat ggctatcgcc aactgctgga acaagttaaa gcaaccggag ttaatgtctg
    10981 ggtacaggat ggcagcggcg tggataaact taccgctgaa cagcgtgaac gttatctaca
    11041 ggccagcgcc gattgccaaa gctccgcccc agcaagcggc attgtttatg aactgtttgt
    11101 cgccggcaaa ggcaaaacct ttacagcgaa accgaaaccg gacgcagaaa ttgcctcgct
    11161 gttagcgaaa cgttcctctt gcggtaaaga catgctctat ttctctctgc gctatttgcc
    11221 cgtcgcgcac ggcattctcg agtattaaat cccctccagg taagtcgggt acgacctggc
    11281 ttacctcttt cgctctcgtc agaattccat caagattaat tgctaaaaag catgctaaag
    11341 aaagaagatc gttatcggcg aacgggccga tcactcatgc aggaagcata gatcctttga
    11401 gcggagtttc tgatatctga ggagtgcgaa atgcaattga gcagcagtga accttgcgtg
    11461 gtgatcctga ccgaaaaaga ggtagaggta agcgtcaata accatgctac gtttaccctt
    11521 ccgaaaaact acctggccgc cttcgcgtgc aacaataacg tcattgaact ctcaacgtta
    11581 aatcacgtat taatcaccca catcaaccgt aacatcatca acgattatct gttgttttta
    11641 aataagaaat taacctgtgt aaagccctgg tcgcggctag caaccccggt tatcgcttgt
    11701 catagccgta caccggaagt gttccggcta gccaccaacc acagcaagca gcaatccagc
    11761 aaaccctgcg aagcggagtt gacgcgcgca ttacttttta ccgtattgtc taactttctt
    11821 gagcaatcgc ggtttattgc cctactgatg tatatcttac gcagcagcgt ccgcgacagc
    11881 gtctgccgca ttattcaaag cgatattcag cattactgga atctgcgaat tgtcgccagt
    11941 tcgctatgtt taagccccag cctgctcaaa aagaaattaa aaaacgaaaa taccagctat
    12001 agccagattg tcacagagtg tcgtatgcgt tacgccgtac agatgttatt gatggataac
    12061 aaaaatatca ctcaggtggc gcaattatgt ggctatagca gcacgtcgta ctttatctct
    12121 gtttttaagg cgttttacag cctgacaccg ttgaattatc tcgccaaaca gcgacaaaaa
    12181 gtgatgtggt gaagggcaaa gcggaaacgg ataagacggg cataaatgaa gaagaaatgg
    12241 cgcgccctgc aggattcgaa cctgcggccc acgacttaga aggtcgttgc tctatccaac
    12301 tgagctaagg gcgcgttgat accgcaatgc ggtgtaatcg cgtgaattat acggtcaacc
    12361 cttgctgagt caatggcttt tgatctggtt gctgaacaag tgaacgaccg cgtctgattt
    12421 tctgatttat ttcgctatag cggcaaacaa acgcacaccg ctgcgcgtct gaatcaagaa
    12481 aacccgtatt ttcatgtatc aaagtacaat ttaccgccct aacggaaaat tgtccgctcc
    12541 tatgagactg gtaactatga aaccaacgtc ggtgatcatt atggatactc atcctatcat
    12601 cagaatgtct attgaagttc tgttgcaaaa aaacagtgaa ttgcagattg tcctgaaaac
    12661 ggatgattac cgcataacca tcgattatct ccgaacccgt cctgttgatt taatcattat
    12721 ggatatagac ttgcccggaa cagacggttt taccttcct
//
LOCUS       DAJTRC010000100        12696 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.100, whole genome shotgun sequence.
ACCESSION   DAJTRC010000100 DAJTRC010000000
VERSION     DAJTRC010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12696
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.100"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(100..738)
                     /gene="rutR"
                     /locus_tag="OO357_003202"
     CDS             complement(100..738)
                     /gene="rutR"
                     /locus_tag="OO357_003202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator RutR"
                     /protein_id="HCR5888043.1"
                     /translation="MTQGAVKTTGKRSRAVSAKKKAILSAALDTFSQFGFHGTRLEQI
                     AELAGVSKTNLLYYFPSKEALYIAVLRQILDIWLAPLKAFREDFAPLAAIKEYIRLKL
                     EVSRDYPQASRLFCMEMLAGAPLLMDELTGDLKALIDEKSALIAGWVKSGKLAPIDPQ
                     HLIFMIWASTQHYADFAPQVEAVTGATLRDEVFFNQTVENVQRIIIEGIRPR"
     gene            1026..2117
                     /gene="rutA"
                     /locus_tag="OO357_003203"
     CDS             1026..2117
                     /gene="rutA"
                     /locus_tag="OO357_003203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309285.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein A"
                     /protein_id="HCR5888044.1"
                     /translation="MKIGVFVPIGNNGWLISTHAPQYMPTFELNKAIAQKAEHYHFDF
                     ALSMIKLRGFGGKTEFWDHNLESFTLMAGLAAVTSRIQIYATAATLTLPPAIVARMAA
                     TIDSISGGRFGVNLVTGWQKPEYEQMGIWPGDDYFSRRYDYLTEYVQVLRDLWGTGKS
                     DFKGDFFTMNDCRVSPQPSIPMKVICAGQSDAGMAFSAQYADFNFCFGKGVNTPTAFA
                     PTAARMKQATEQTGRDVGSYVLFMVIADETDDATRAKWEHYKAGADEEALSWLTEQSQ
                     KDTRSGTDTNVRQMADPTSAVNINMGTLVGSYASVARMLDEVASVPGAEGVLLTFDDF
                     LSGIETFGERIQPLMQCRAHLSALTQEVA"
     gene            2117..2809
                     /gene="rutB"
                     /locus_tag="OO357_003204"
     CDS             2117..2809
                     /gene="rutB"
                     /locus_tag="OO357_003204"
                     /EC_number="3.5.1.110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415531.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxyureidoacrylate/ureidoacrylate
                     amidohydrolase RutB"
                     /protein_id="HCR5888045.1"
                     /translation="MTTLTARPEAITFDPQQSAQIVVDMQNAYATPGGYLDLAGFDVS
                     TTRPVIANIQTAVTAARAAGMLIIWFQNGWDEQYVEAGGPGSPNFHKSNALKTMRKQP
                     QLQGKLLAKGSWDYQLVDELVPQPGDIVLPKPRYSGFFNTPLDSILRSRGIRHLVFTG
                     IATNVCVESTLRDGFFLEHFGVVLEDATHQAGPEFAQKAALFNIETFFGWVSDVETFC
                     DALSPTSFARIA"
     gene            2821..3207
                     /gene="rutC"
                     /locus_tag="OO357_003205"
     CDS             2821..3207
                     /gene="rutC"
                     /locus_tag="OO357_003205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein C"
                     /protein_id="HCR5888046.1"
                     /translation="MPKSVIIPAGSSAPLAPFVPGTLADGVVYVSGTLAFDQHNNVLF
                     ADDPKAQTRHVLETIRKVIETAGGTMADVTFNSIFITDWKNYAAINEIYAEFFPGDKP
                     ARFYIQCGLVKPDALVEIATIAHIAK"
     gene            3215..4015
                     /gene="rutD"
                     /locus_tag="OO357_003206"
     CDS             3215..4015
                     /gene="rutD"
                     /locus_tag="OO357_003206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization protein D"
                     /protein_id="HCR5888047.1"
                     /translation="MKLSLSSPPYADAPVVVLISGLGGSGSYWLPQLAVLEQEYQVVC
                     YDQRGTGNNPDTLAEDYSIAQMAAELHQALVAAGIERYAVVGHALGALVGMQLALDYP
                     ASVTMLVSVNGWLRINAHTRRCFQVRERLLYSGGAQAWVEAQPLFLYPADWMAARAPR
                     LEAEDALALAHFQGKNNLLRRLNALKRADFSHHADRIRCPVQIICASDDLLVPTACSS
                     ELHAALPDSQKMVMPYGGHACNVTDPETFNALLLNGLASLLHHREAAL"
     gene            4025..4615
                     /locus_tag="OO357_003207"
     CDS             4025..4615
                     /locus_tag="OO357_003207"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HCR5888048.1"
                     /translation="MNEAVSPGALSTLFTDARTHNGWRETPVSDETLQELYALMKWGP
                     TSANCSPARIVFIRTAEGKERLRPALSSGNLQKTLTAPVTAIVAWDSEFYERLPLLFP
                     HGDARSWFTSSPQLAEETAFRNSSMQAAYLIVACRALGLDTGPMSGFDRQHVDDAFFA
                     GSTLKSNLLINIGYGDSSKLFARLPRLSFEEACGLL"
     gene            4626..5120
                     /gene="rutF"
                     /locus_tag="OO357_003208"
     CDS             4626..5120
                     /gene="rutF"
                     /locus_tag="OO357_003208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization flavin reductase protein
                     F"
                     /protein_id="HCR5888049.1"
                     /translation="MNIVDQQTFRDAMSCMGAAVNIITTDGPAGRAGFTASAVCSVTD
                     TPPTLLVCLNRGASVWPVFNENRTLCVNTLSAGQEPLSNLFGGKTPMEHRFAAARWQT
                     GVTGCPQLEEALVSFDCRISQVVSVGTHDILFCAIEAIHRHTTPYGLVWFDRSYHALM
                     RPAC"
     gene            5141..6469
                     /gene="rutG"
                     /locus_tag="OO357_003209"
     CDS             5141..6469
                     /gene="rutG"
                     /locus_tag="OO357_003209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415526.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine utilization transport protein G"
                     /protein_id="HCR5888050.1"
                     /translation="MAMFGFPHWQLKSTSTESGVVAPDERLPFAQTAVMGVQHAVAMF
                     GATVLMPILMGLDPNLSILMSGIGTLLFFFITGGRVPSYLGSSAAFVGVVIAATGFNG
                     QGINPNISIALGGIIACGLVYTVIGLVVMKIGTRWIERLMPPVVTGAVVMAIGLNLAP
                     IAVKSVSASAFDSWMAVMTVLCIGLVAVFTRGMIQRLLILVGLIVACLLYGVMTNVLG
                     LGKAVDFTLVSYAAWFGLPHFSTPGFNGQAMMLIAPVAVILVAENLGHLKAVAGMTGR
                     NMDPYMGRAFVGDGLATMLSGSVGGSGVTTYAENIGVMAVTKVYSTLVFVAAAVIAML
                     LGFSPKFGALIHTIPAAVIGGASIVVFGLIAVAGARIWVQNRVDLSQNANLIMVAVTF
                     VLGAGDFALTLGGFTLGGIGTATFGAILLNALLSRRLVDVPPPEVVHQEP"
     gene            complement(6551..6658)
                     /locus_tag="OO357_003210"
     CDS             complement(6551..6658)
                     /locus_tag="OO357_003210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415525.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888051.1"
                     /translation="MANHRGGSGNFAEDRERASEAGKKGGKSSHGKSDN"
     gene            7031..7627
                     /gene="wrbA"
                     /locus_tag="OO357_003211"
     CDS             7031..7627
                     /gene="wrbA"
                     /locus_tag="OO357_003211"
                     /EC_number="1.6.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H:quinone oxidoreductase"
                     /protein_id="HCR5888052.1"
                     /translation="MAKVLVLYYSMYGHIETMARAVAEGASKVDGAEVVVKRVPETMP
                     PQLFEKAGGKTQTAPVATPQELADYDAIIFGTPTRFGNMSGQMRTFLDQTGGLWASGA
                     LYGKLASVFSSTGTGGGQEQTITSTWTTLAHHGMVIVPIGYAAQELFDVSQVRGGTPY
                     GATTIAGGDGSRQPSQEELSIARYQGEYVAGLAVKLNG"
     gene            7648..7875
                     /locus_tag="OO357_003212"
     CDS             7648..7875
                     /locus_tag="OO357_003212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002898701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccJ family protein"
                     /protein_id="HCR5888053.1"
                     /translation="MPTQEAKAHHVGEWASLRNTSPEIAEAIFEVAGYDEKMAEKIWE
                     EGSDEVLVKAFAKTDKDSLFWGEQTIERKNV"
     gene            complement(7913..9154)
                     /gene="agp"
                     /locus_tag="OO357_003213"
     CDS             complement(7913..9154)
                     /gene="agp"
                     /locus_tag="OO357_003213"
                     /EC_number="3.1.3.10"
                     /EC_number="3.1.3.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glucose-1-phosphatase/inositol
                     phosphatase"
                     /protein_id="HCR5888054.1"
                     /translation="MNKTLIAATVAGIVLLASNAQAQTVPEGYQLQQVLMMSRHNLRA
                     PLANNGSVLEQSTPNKWPEWDVPGGQLTTKGGVLEVYMGHYMREWLAEQGIVKSGECP
                     PPDTVYAYANSLQRTVATAQFFITGAFPGCDIPVHHQEKMGTMDPTFNPMITDDSAAF
                     SEQAVAAMEKELSKLQLTDSYQLLEKIVNYKDSPACKEKQQCSLVDGKNTFSAKYQQE
                     PGVSGPLKVGNSLVDAFTLQYYEGFPMDQVAWGEIKSDQQWKVLSKLKNGYQDSLFTS
                     PEVARNVAKPLVSYIDKALVTDRASAPKITVLVGHDSNIASLLTALDFKPYQLHDQNE
                     RTPIGGKIVFQRWHDSKANRDLMKIEYVYQSAEQLRNADALTLQAPAQRVTLELSGCP
                     IDANGFCPMDKFDSVLNEAVK"
     gene            complement(9447..10703)
                     /locus_tag="OO357_003214"
     CDS             complement(9447..10703)
                     /locus_tag="OO357_003214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccE family protein"
                     /protein_id="HCR5888055.1"
                     /translation="MGSNIHGISCTANNYLKQAWNDIKNEYEKNQTYSITLFENTLVC
                     FMRLYNELRRKVNEEDTPCLECESLEKEFEEMQNDNDLSLFMRTLRTNDTQIYSGVSG
                     GITYTIQYVQDVDIVRVSLPGRGSESITDFKGYYWYGFMEYIENINACDDVFSEYCLD
                     NENMSIQPEQINMPGISDLDTGIDLSGISFIQSEINKTYGLKYAPVDGDGYCLLRAIL
                     VLKEHEYSWALGSHKTQKQVYEEFIKIVDKQTIEALVDTAFYNLREDVKTLFGVDLQS
                     DNKIQGVGSFMSWSFLFFKKQFIDSCLNDKKCILHLPEFIFNDNKNLLALDTDTSDRI
                     KAVKNFLAVLSDSICSLFIVNSNVASISLGNESFSTDEDLEYGYLTNTGNHYDVYLPP
                     ELFSQAYKLKNKEMNAQLDYLNRYAT"
     gene            10964..11884
                     /gene="cbpA"
                     /locus_tag="OO357_003215"
     CDS             10964..11884
                     /gene="cbpA"
                     /locus_tag="OO357_003215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curved DNA-binding protein"
                     /protein_id="HCR5888056.1"
                     /translation="MELKDYYAIMGVKPTDDLKTIKTAYRRLARKYHPDVSKEPDAEA
                     RFKEVAEAWEVLSDEQRRAEYDQMWQHRNDPQFNRQFHHGDGQSFNAEDFDDIFSSIF
                     GQHARQSRQRPAARGHDIEIEVAVFLEETLTEHKRTISYNLPVYNAFGMIEQEIPKTL
                     NVKIPAGVGNGQRIRLKGQGTPGENGGPNGDLWLVIHIAPHPLFDIVGQDLEIVVPVS
                     PWEAALGTKVTVPTLKESILLTIPPGSQAGQRLRVKGKGLVSKKQTGDLYAVLKIVMP
                     PKPDENTAALWQQLADAQSSFDPRKDWGKA"
     gene            11884..12189
                     /gene="cbpM"
                     /locus_tag="OO357_003216"
     CDS             11884..12189
                     /gene="cbpM"
                     /locus_tag="OO357_003216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperone modulator CbpM"
                     /protein_id="HCR5888057.1"
                     /translation="MANVTVTFTITEFCLHTGISEEELNEIVGLGVVEPREIQETTWV
                     FDDHAAIVVQRAVRLRHELALDWPGIAVALTLMDDIAHLKQENRLLRQRLSRFVAHP"
     gene            complement(12282..>12696)
                     /gene="torD"
                     /locus_tag="OO357_003217"
     CDS             complement(12282..>12696)
                     /gene="torD"
                     /locus_tag="OO357_003217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5888058.1"
                     /translation="TLTVRDDARLELAADFCGLFLMTDKQAALPYASAYKQDEQEIKR
                     LLVEAGMETSGNFNEPADHLAIYLELLSYLHFSLGEGTVPARRIDSLRQKTLTALRQW
                     LPEFAARCRQYDSFGFYAALSQLLLVLVECDHQNR"
BASE COUNT         3031 a         3241 c         3200 g         3224 t
ORIGIN      
        1 tcgctgagtg tgaataccgc tgccgctggc ggtaacgcca gtttaatgac gatcggttaa
       61 acaggtagcc ggaggatgtt acaacctcct ccgtcatctt taacgtggtc gaatcccctc
      121 aataataatc cgctgcacgt tttcaaccgt ttgattgaaa aatacctcat cgcgcaacgt
      181 cgcgcctgtc accgcctcca cctgaggggc gaaatcggcg taatgttgag tggaagccca
      241 aatcataaaa atcaaatgct gcggatcaat cggcgcgagt ttgccgcttt tgacccaacc
      301 ggcaatcagc gccgatttct catcaattaa tgccttcaaa tcgcccgtca gttcatccat
      361 taacaacggc gcgcctgcca gcatctccat acagaacagg cgcgaagcct gcggataatc
      421 gcgtgagact tccagcttca gacggatgta ctcttttatc gccgccagcg gggcgaaatc
      481 ttcacgaaac gcttttaacg gtgccagcca gatatcgaga atctgccgca gcacggcaat
      541 atacagcgcc tcttttgacg ggaagtaata cagcagattg gtttttgaaa cacccgccaa
      601 ctctgcgatc tgctccagcc ttgtgccgtg aaaaccgaat tgtgaaaaag tgtccagtgc
      661 tgcgctaaga atcgcttttt tcttcgcgct tactgcgcgc gaacgtttac ccgttgtttt
      721 cactgcgcct tgcgtcatgc gctctcccct cttgtagatt ggtaacagga tagcaaaacc
      781 cccgcccggc ctcgaccttc tgcactctca tcgcgcacgg atgcatgttt tatgtgcaac
      841 tgttttgacc gtttagtcca ctttttacca gatattttag ataacacaat gctaacacat
      901 taataatatt cactttttaa aaactggcat ccgctttgcg aacaagccaa tacgcggctg
      961 aaaaggatat gcaggatgca gcgccccgtc tgactttcac tttacgagac gaagagaggt
     1021 tgatgatgaa aattggcgta ttcgtaccta ttggcaacaa cggctggctc atttcgaccc
     1081 acgcgccgca gtacatgccg acctttgaac tgaataaagc cattgcgcaa aaagcggagc
     1141 attaccattt cgatttcgcc ctgtcgatga tcaaactgcg tggctttggc ggcaaaactg
     1201 agttctggga tcacaacctt gagtcgttca ccttgatggc gggtctggca gccgtgacct
     1261 cgcgcattca gatttacgcc actgctgcca ccttaacgtt acctccggca atcgtcgccc
     1321 gtatggccgc aaccatcgac tccatctctg gcgggcgttt tggcgtcaac ctcgtgactg
     1381 gctggcaaaa gcccgagtat gagcagatgg gtatctggcc tggcgatgac tatttctccc
     1441 gtcgttacga ctatctcacc gaatatgttc aggtgctgcg cgacctgtgg ggcacgggga
     1501 aaagcgattt taaaggcgat tttttcacca tgaatgattg tcgcgtcagt ccgcaaccga
     1561 gtatccccat gaaagtgatc tgcgccgggc aaagcgacgc tggcatggcg ttctccgccc
     1621 agtatgccga tttcaacttc tgtttcggca aaggcgtaaa tacacccacg gctttcgccc
     1681 cgaccgctgc gcggatgaaa caggccacag agcaaaccgg acgcgacgtt ggctcttatg
     1741 tgttgtttat ggtgattgcc gatgaaaccg acgatgccac tcgcgccaaa tgggaacact
     1801 acaaagcggg cgcggatgaa gaggcgttaa gctggctaac cgaacaaagt cagaaagata
     1861 cccgctccgg tactgacacc aacgttcgtc agatggccga tcccacttcg gcggtaaaca
     1921 tcaatatggg gacgttagtc ggttcttacg ccagtgtcgc gcgcatgtta gatgaagtcg
     1981 caagcgtgcc tggtgcagaa ggcgtgctgt tgaccttcga tgattttctg tcgggaatcg
     2041 aaaccttcgg cgagcgcatt caaccactga tgcagtgccg cgcccatctc tctgcgctga
     2101 ctcaggaggt ggcatgatga caaccttaac cgctcgaccg gaagccatta ccttcgatcc
     2161 gcagcaaagt gcgcagatcg tggtggatat gcaaaacgct tatgccacgc caggcggcta
     2221 cttagatctc gccgggtttg atgtctcaac cactcgcccg gtcattgcca acattcaaac
     2281 cgctgtgacc gcagcgcgag cggcagggat gttgatcatc tggtttcaaa atggctggga
     2341 tgaacagtat gtcgaggctg gcggccccgg ctcaccgaat tttcataaat cgaacgccct
     2401 gaaaaccatg cgtaagcagc cgcagctgca ggggaaattg ctggcgaaag gctcctggga
     2461 ttatcaactg gtggatgaac tggtgccgca gcctggcgat attgtgctgc cgaagccgcg
     2521 ctacagcggt ttcttcaata cgccgctgga cagcatttta cgcagccgtg gaatacgcca
     2581 tctggttttc accggtatcg ctaccaacgt ctgcgtcgaa tcgacgctac gcgatggctt
     2641 ttttctggag catttcggcg tggtgctgga agacgcaaca caccaggcgg ggccggaatt
     2701 tgcacagaaa gccgcgttgt tcaatatcga aacctttttt ggctgggtca gcgacgtcga
     2761 aacgttctgc gacgcgcttt ctcccacgtc ctttgctcgt atcgcttaag gagtttagcg
     2821 atgccaaaat ccgtaattat tcccgctggc agcagcgcac cgctggcccc cttcgttccc
     2881 ggcacgctgg ctgatggcgt ggtgtatgtc tccggtacgc tggcttttga tcaacataat
     2941 aacgtgctgt ttgccgatga cccaaaggcg caaacccgcc acgttctgga aactatccgc
     3001 aaggtgatcg agacggcggg tggcacgatg gcggatgtga ccttcaacag catctttatt
     3061 accgactgga aaaattacgc cgcgattaac gaaatctacg ccgagttttt tccgggtgat
     3121 aaaccggcgc gattctacat tcagtgcgga ctggtaaaac ctgacgcgct ggtggaaatc
     3181 gccacaattg cgcatatcgc caagtgaggc cgcgatgaaa ctttcactct catctccccc
     3241 ttatgctgat gcacccgtag tggtgttgat ttcgggtctt ggcggtagcg gcagttactg
     3301 gttaccgcaa ctggcggtgc tggagcagga gtatcaggta gtctgttacg accagcgcgg
     3361 caccggcaat aatcccgaca cactggcaga agattacagt atcgcccaga tggcagcgga
     3421 actgcatcag gcgctggtag ccgcagggat tgagcgttac gcggtggtcg gccatgcgct
     3481 cggtgcgctg gtggggatgc agctggcgct ggattatccc gcgtcggtaa ctatgctggt
     3541 cagcgttaac ggctggctac gaataaacgc ccatacgcgc cgctgttttc aggttcgcga
     3601 acgattactg tatagcggcg gcgcgcaagc atgggtggaa gcgcagccgt tgttcctcta
     3661 tcccgccgac tggatggcgg cccgcgcacc tcgcctggag gcagaagacg cgctggcact
     3721 ggcgcatttt cagggcaaaa ataatttact acgtcgactt aacgccctca aacgcgctga
     3781 ctttagtcac catgcggatc gcatccgctg cccggtgcaa atcatctgcg ccagtgatga
     3841 tctgctggtg ccaacagcat gttccagtga acttcatgcc gccctgcccg atagccagaa
     3901 aatggtgatg ccctatggcg gacacgcctg caacgtgacc gatcccgaaa cgtttaatgc
     3961 tctgttactc aacgggcttg ccagcctgtt acatcaccgt gaagccgccc tgtaaggaat
     4021 tgctatgaac gaagccgtta gcccaggtgc gcttagcacc ctgttcaccg atgcccgcac
     4081 tcacaacggc tggcgggaga cacccgtcag cgatgagacg ttacaggagc tttatgccct
     4141 gatgaaatgg gggccgacat cagctaactg ttctccggca cggatcgtgt ttatccgcac
     4201 tgcagaagga aaagaacgtc tgcgcccggc gctttccagc ggcaatctgc aaaaaaccct
     4261 gaccgcgccc gtcaccgcta tcgtcgcctg ggacagtgaa ttttatgaac ggttaccact
     4321 actgtttccc cacggtgatg cccgcagttg gtttacctcc agcccacaac ttgccgaaga
     4381 aacagcgttt cgcaacagtt ccatgcaggc ggcttatctg atcgtcgctt gccgtgcgct
     4441 gggactggat accggcccga tgtcgggttt tgaccgtcaa cacgttgacg acgccttttt
     4501 tgcgggcagc acgctgaaga gcaatctgct gattaatatc ggctatggcg atagcagcaa
     4561 actttttgcg cgcctgccac gtctgtcctt tgaagaagcc tgcgggctgt tgtaaggagc
     4621 aaactatgaa catagttgat caacaaacct ttcgcgatgc gatgtcctgt atgggcgcgg
     4681 cggtcaatat catcaccacg gacggtccag ccgggcgcgc cgggttcacc gccagcgccg
     4741 tctgcagcgt gaccgacacg ccgcccacat tactggtgtg cctgaatcgt ggggcgtccg
     4801 tctggccggt attcaatgaa aatcgaacgc tgtgtgtaaa tacactgagc gccgggcagg
     4861 agccgctttc aaatcttttt ggcggcaaaa cgcccatgga acaccgcttt gccgccgccc
     4921 gctggcagac gggtgtgacc ggatgcccgc aactggaaga ggctctggtt tcgtttgact
     4981 gccgtatcag ccaggtggtg agcgtcggca cccacgacat tctgttttgc gccatcgaag
     5041 cgattcatcg tcacaccaca ccctacgggc tggtgtggtt cgatcgcagt tatcacgcgc
     5101 tgatgcgccc tgcttgttaa cctccttaag gagacagctg atggcaatgt tcggttttcc
     5161 tcactggcag ttaaaatcga cctctacaga gagcggcgtg gtcgcgccgg atgaacgatt
     5221 gccgtttgct cagacggcag taatgggcgt tcaacacgcg gtggcgatgt ttggcgcgac
     5281 ggtgttaatg ccgattctga tggggctgga tcccaatctt tccattttaa tgtcggggat
     5341 cggcacgtta ctgtttttct ttattaccgg cgggcgcgtc cccagttatc tcggctccag
     5401 cgcggctttt gtcggcgtgg tgattgccgc gacgggcttt aacggtcagg gcattaaccc
     5461 gaatatcagc atcgcgctgg gcgggattat cgcctgtggg ctggtttata ccgtcattgg
     5521 cctggtggtg atgaaaattg gtacacgctg gattgaacga ctaatgccgc cggtggtgac
     5581 gggtgcggtg gtgatggcga ttggcctgaa cttagcgcct atcgcagtga aaagtgtatc
     5641 ggcttcggcc tttgatagct ggatggcggt aatgacggtg ctgtgtattg ggctggtggc
     5701 ggtgtttacc cgtggcatga tccagcggtt attgattctc gtaggattaa ttgtcgcctg
     5761 tctgctgtat ggcgtgatga ccaacgtttt agggctggga aaagccgtcg attttactct
     5821 cgtcagctat gctgcctggt tcggtctgcc gcatttttcc actcctggtt ttaatggcca
     5881 ggcgatgatg ttgattgcgc ccgtggcggt gattctggtg gcggaaaact taggtcatct
     5941 caaggcggtc gccgggatga ccggacgcaa tatggacccg tatatggggc gagcattcgt
     6001 cggtgatggt ctggcaacga tgctttccgg ctctgtcggc ggtagcggcg tcaccaccta
     6061 tgctgaaaat atcggtgtga tggccgtgac aaaagtctac tcgacgctgg tgtttgttgc
     6121 agctgccgtc atcgctatgc tgttgggctt ttcgccgaaa tttggcgcgc tgatccatac
     6181 cattccggcc gcggtgattg gtggtgcatc aattgttgtg ttcgggctga ttgccgtcgc
     6241 aggcgcaagg atatgggtac aaaaccgtgt cgatttaagc cagaacgcta atttgattat
     6301 ggtcgcagta accttcgtgc tgggcgcggg tgattttgct ctcacgcttg gcggttttac
     6361 gttgggaggg atcggtacag caacctttgg cgcgatttta ctcaatgcgt tattaagcag
     6421 aaggttggtt gacgttccac cgccagaagt tgttcatcag gagccataaa cagaaaaccg
     6481 ccgggttacc ggcggtgatg actttttgac gagcttaaga aaaaaatgca ttcgtcattg
     6541 attagcccgg ctagttgtcg cttttgccgt gactgctctt gccacctttt ttacctgctt
     6601 ctgatgctct ttcgcggtct tctgcaaaat tgccggaacc gcctcgatgg tttgccatta
     6661 ctgacctccg tctgggtata gacatcatat tgcatagaat taacgcggcg tatggcggct
     6721 gccatatttt cgctacgtgg gactaagctt agtgaactga cgcggacaga cccgcgtttg
     6781 gtaatatcct gcaacagctt cttagcgaga atagatatca tctccaataa ttatccataa
     6841 gccgctctta tgattaaaaa aacgcgatga gataaaaatg tgtcattttg cgacaaaatt
     6901 acgtgcttgt gaaagttgtt ataaatcaaa taagtggttg tgaaatttgc actctgaaaa
     6961 ggacgtctta tctttaaata agtggtagcg aatcgctacg gaatagagat aacacgagga
     7021 gtggttagaa atggctaaag ttctggtgct ttattattcc atgtacggac atattgaaac
     7081 gatggcacgc gcagtcgctg agggtgcaag caaagtggat ggcgcagaag ttgtcgttaa
     7141 gcgtgtaccg gaaaccatgc cgccgcaatt atttgaaaaa gcaggcggta aaacgcaaac
     7201 tgcaccggtt gcaaccccgc aagaactggc cgattacgac gccattattt ttggtacacc
     7261 tacccgcttt ggcaacatgt ccggtcaaat gcgtaccttc ctcgaccaga cgggcggcct
     7321 gtgggcttcc ggcgcactat acggaaaact ggcgagcgtc tttagttcca ccggtactgg
     7381 cggcggtcag gaacaaacca tcacatccac ctggacgacc cttgcgcatc acggcatggt
     7441 gattgtcccc attggctacg cagcgcagga attatttgac gtttcacagg ttcgcggcgg
     7501 tacgccgtac ggcgcaacca ccatcgcagg cggtgacggc tcacgccagc caagccagga
     7561 agaactgtct attgctcgtt atcaagggga atatgtcgca ggtctggcag ttaaacttaa
     7621 cggctaatct tcaacaggag gatacgcatg ccaactcaag aagcgaaagc tcatcacgtc
     7681 ggtgaatggg catctctgcg caatacgtcg ccggaaatag ccgaggccat tttcgaagtc
     7741 gccgggtacg acgaaaaaat ggcagaaaag atttgggaag aaggtagtga tgaagtgtta
     7801 gtcaaagcct ttgctaagac cgataaagat tcgctttttt ggggcgaaca gaccatcgaa
     7861 cgtaaaaacg tttaaccagc gactcccccg cttctcgcgg gggagttttc tgttatttca
     7921 ccgcctcatt caacacgcta tcaaacttat ccatcgggca gaaaccgttg gcatctatcg
     7981 ggcaaccgct taattccagc gtcacgcgct gcgcaggtgc ctgcagggtt aacgcatcgg
     8041 cattacgtaa ctgttccgca ctctggtaca catattcaat tttcatcaaa tcgcgattgg
     8101 ctttgctgtc atgccaacgc tggaaaacga ttttgccgcc aatcggcgtg cgttcgttct
     8161 ggtcatgcaa ctgatacggt ttgaaatcca gcgccgttaa cagagaggct atgttggagt
     8221 cgtgcccaac taacaccgtg attttcggag cactggcgcg atcggtgacc agggctttgt
     8281 cgatataact gaccagcggt ttcgcaacat tgcgcgccac ttccggtgag gtaaacagac
     8341 tgtcctggta accgtttttc agcttcgaca ataccttcca ctgctggtca gatttgattt
     8401 ctccccaggc cacctgatcc atcggaaaac cttcgtagta ttgcaaagta aacgcatcta
     8461 ccagcgagtt gccgactttc agcggcccgg aaacacctgg ttcttgttga tacttggcgc
     8521 taaaggtatt tttgccatcc accagcgaac actgttgttt ctctttacag gcaggggaat
     8581 ctttatagtt aacgattttt tccagtagct ggtagctgtc ggtaagctgg agtttgctga
     8641 gctctttctc cattgccgcc accgcctgtt cactgaatgc ggcggaatca tcggtgatca
     8701 tcgggttaaa ggttgggtcc atagtgccca ttttttcctg gtgatgcaca ggaatatcac
     8761 acccagggaa tgcaccagta ataaagaact gggcggtagc gacggtacgt tgcagactat
     8821 tggcataggc ataaacggtg tccggcggcg ggcattcccc cgatttcact atcccctgct
     8881 ctgccagcca ttcacgcatg taatggccca tatacacttc gagcacgcca cctttggtgg
     8941 tgagttgccc accggggacg tcccattcgg gccatttatt cggcgtcgac tgctccagca
     9001 cactgccatt gttcgccagc ggcgcacgta agttatggcg gctcatcatg agcacttgct
     9061 gtagctgata gccttccggt acggtttgtg cctgagcatt tgaagcgagt aaaactatcc
     9121 ctgccacagt tgcggcgatt agcgttttgt tcattcctgg cacctctttt gttatcaatc
     9181 attaaagagt gtgacagaaa tatgactttt ttccgggaac aggttcgcaa aatggcttgt
     9241 tgaacgcctg tttttctata tacatcttca aaaacaggca aggtttgacg caaactatgc
     9301 ctgattagcc cctgcctcgc gtacgccgct gataattgca cctgtggcta aacagaatat
     9361 ctgcggcgtt accaatacag agacaatatt taccaataaa aaagtgtgag actaataaca
     9421 atggttttta gcatatgcct ttgccattaa gttgcataac gatttaaata gtcaagttgc
     9481 gcattcattt ccttattttt taacttataa gcctgagaaa aaagttcagg agggaggtaa
     9541 acatcataat gattgccagt gtttgttaaa tatccatact caagatcttc atctgttgaa
     9601 aaggattcat ttcccaagga gattgatgcc acattactat taacaataaa taatgagcaa
     9661 atgctatctg aaagaacagc aagaaaattc ttcaccgctt taatcctatc cgacgtgtcg
     9721 gtatctaaag caagcaaatt cttgttatca ttaaatataa actcgggtaa atgcagtatg
     9781 cattttttgt cattcaagca actatcaatg aattgttttt taaaaaacag aaagctccat
     9841 gacataaaac tacccacacc ctgaattttg ttgtcagatt gcagatcaac gccaaataat
     9901 gtctttacat cttccctgag attatagaat gccgtatcaa caagagcctc gatcgtttgt
     9961 ttatcaacaa ttttaataaa ctcttcgtat acttgctttt gcgtcttatg gctgcccagc
    10021 gcccatgaat actcatgttc ttttaaaacc agtatggctc ttaacagaca atatccatcg
    10081 ccatctactg gagcgtattt taatccatat gttttattaa tttcactctg aataaaagat
    10141 atgccagaca aatcaatacc agtatctaaa tcagatattc ccggcatatt tatctgttct
    10201 ggctggatac tcatattttc attatctaaa caatactcag aaaaaacatc atcacacgca
    10261 ttaatgtttt caatatattc cataaagcca taccaataat aacctttaaa atctgtgata
    10321 gactctgaac ctctgcccgg caaggacact ctaacaatat caacatcttg aacatattgt
    10381 atagtatatg taatacctcc tgaaacccct gaataaattt gtgtatcatt agtacgcaat
    10441 gttctcataa ataatgatag atcattatca ttctgcattt cctcaaattc tttttctagt
    10501 gattcacatt ccagacatgg agtatcctct tcatttactt tacgtctgag ttcattgtac
    10561 aaccgcataa aacacaccag tgtgttttca aaaagcgtga ttgaatatgt ttgatttttt
    10621 tcgtactcat tttttatatc gttccaggcc tgtttcaaat aattatttgc agtgcaacta
    10681 attccatgta tattactacc catatatagc gtctataaaa tttaataaat aatgacgccc
    10741 tagttaaact taaagtgcct ggttcaacta tcaaaaatcg ctcacccttt ttcacctgtt
    10801 taaaatatgt tcagcaaccc atcttgatgg cgacctcctc tccgcgatga tttcaataac
    10861 atattctgtg ttggcatatg aaattttgag gattacccta cacttatagg agttacctta
    10921 caggggttcc ttcaatttgt gttgatttac gcgagataac gctatggaat taaaggatta
    10981 ttacgccatc atgggcgtga aaccgacgga cgatctcaag acaatcaaga ccgcctatcg
    11041 tcgactggcc cgtaaatacc atcctgatgt cagcaaagaa ccggatgccg aagcccgctt
    11101 caaagaggtc gctgaagcct gggaagtgtt aagtgatgaa caacgtcgcg ctgagtatga
    11161 tcagatgtgg caacatcgca acgatccgca atttaaccgt cagttccatc atggcgacgg
    11221 tcagagtttt aatgccgaag attttgacga tatcttctcg tcaattttcg gtcagcatgc
    11281 ccgccagagc cgtcaacgcc ccgccgcacg tggccacgat attgaaatcg aagtggcggt
    11341 attcctcgaa gaaacgctta ctgagcataa gcgtaccatc agctataacc tgccggttta
    11401 taacgccttt ggcatgatcg aacaggaaat cccgaaaacg ctgaatgtga agatcccggc
    11461 aggcgtcggc aatggtcaac gcatccgcct gaaaggccag gggacgccgg gcgaaaacgg
    11521 cggtccaaat ggcgatttgt ggctggtgat tcatattgcg ccacatccgc tgttcgatat
    11581 tgtcggccag gatctggaaa ttgtggtgcc ggttagcccg tgggaagcgg cgctgggtac
    11641 taaagtcacc gttccaacac tgaaagaaag cattttgctg actatcccgc caggcagcca
    11701 ggccgggcaa cgattgcgcg ttaaaggcaa aggtctggtg agcaaaaaac agaccggcga
    11761 tctgtatgcg gtactgaaaa tcgtgatgcc gccgaaaccg gatgaaaaca ctgccgcgct
    11821 gtggcagcaa ctggcagacg cccagtcgtc ttttgatcca cgtaaagatt gggggaaagc
    11881 ataatggcta atgttacggt gacttttact attaccgaat tttgcctgca taccggcatc
    11941 tctgaagagg agttgaatga aattgtcggt ttgggggtgg ttgaaccgcg tgagatccag
    12001 gaaacaacct gggtatttga cgaccatgcc gccattgtgg tgcaacgcgc ggtacgcctg
    12061 cgtcatgaac tggctctgga ctggccgggg atcgcggtgg cgctgacgtt aatggatgat
    12121 attgcgcacc tgaagcagga aaaccgcctg ctacgccagc ggctttcccg gtttgtagct
    12181 catccgtgat gtgtgtttgc cggatgtgat gacgcatccg gcaactgcgc cataatgcct
    12241 gatgcgacgc taaacgcgtc ttttcaggcg cataaacgac gttatctgtt ttggtggtcg
    12301 cactccacta acaccagcaa taactggctt agtgccgcgt aaaaaccaaa gctgtcatac
    12361 tgtcgacaac gcgcagcaaa ctctggtaac cattgccgca gcgccgtcag tgttttttgc
    12421 cgcaaactgt cgattcttcg cgcaggaacg gtcccctctc ccagcgaaaa atgcagataa
    12481 ctcaacaact cgagatagat cgccagatga tctgccggtt cgttgaaatt gccactggtt
    12541 tccatccctg cctcaactaa caagcgttta atctcttgct cgtcctgttt gtaggccgat
    12601 gcatacggca gcgccgcttg tttgtcggtc atcagaaaca ggccgcaaaa gtcagcggcc
    12661 agttccagac gggcatcgtc acgtactgtc agcgtg
//
LOCUS       DAJTRC010000101        12652 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.101, whole genome shotgun sequence.
ACCESSION   DAJTRC010000101 DAJTRC010000000
VERSION     DAJTRC010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.101"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            118..1815
                     /gene="maeA"
                     /locus_tag="OO357_003218"
     CDS             118..1815
                     /gene="maeA"
                     /locus_tag="OO357_003218"
                     /EC_number="1.1.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707611.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HCR5888059.1"
                     /translation="MEPKTKKQRSLYIPYAGPVLLEFPLLNKGSAFSMEERRNFNLLG
                     LLPEVVETIEEQAERAWIQYQGFKTEIDKHIYLRNIQDTNETLFYRLVNNHLDEMMPV
                     IYTPTVGAACERFSEIYRRSRGVFISYQNRHNMDDILQNVPNHNIKVIVVTDGERILG
                     LGDQGIGGMGIPIGKLSLYTACGGISPAYTLPVVLDVGTNNQQLLNDPLYMGWRNPRI
                     TDDEYYEFVDEFIQAVKQRWPDVLLQFEDFAQKNAMPLLNRYRNEICSFNDDIQGTAA
                     VTVGTLIAASRAAGGQLSEKKIVFLGAGSAGCGIAEMIIAQTQREGLSEEAARQKVFM
                     VDRFGLLTDKMPNLLPFQTKLVQKRENLSDWDTDSDVLSLLDVVRNVKPDILIGVSGQ
                     TGLFTEEIIREMHKHCPRPIVMPLSNPTSRVEATPQDIIAWTEGNALVATGSPFNPVV
                     WKDKIYPIAQCNNAFIFPGIGLGVIASGASRITDEMLMSASETLAQYSPLVLNGEGLV
                     LPELKDIQKVSRAIAFAVGKMAQQQGVAVKTSAEALQQAIDDNFWQAEYRDYRRTSI"
     gene            1949..2959
                     /gene="adhP"
                     /locus_tag="OO357_003219"
     CDS             1949..2959
                     /gene="adhP"
                     /locus_tag="OO357_003219"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase AdhP"
                     /protein_id="HCR5888060.1"
                     /translation="MKTAVVTKDHHVDVTDKTLRSLKHGEALLKMECCGVCHTDLHVK
                     NGDFGDKTGVILGHEGIGVVAEVGPGVTSLKPGDRASVAWFYEGCGHCEYCNSGNETL
                     CRSVKNAGYSVDGGMEEECIVVADYAVKVPDGLDSAAPSSITCAGVTTYKAVKLSKIR
                     PGQWIAIYGLGGLGNLALQYAKNVFNAKVIAIDVNDEQLKLATEMDADLAINSRTEDA
                     AKIVQEKTGGAHAAVVTAVAKAAFNSAVDAVRAGGRVVAVGLPPESMSLDIPRLVLDG
                     IEVVGSLVGTRQDLTEAFQFAAEGKVVPKVALRPLADINTIFTEMEEGKIRGRMVIDF
                     RH"
     gene            3105..3389
                     /locus_tag="OO357_003220"
     CDS             3105..3389
                     /locus_tag="OO357_003220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415994.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antidote protein"
                     /protein_id="HCR5888061.1"
                     /translation="MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTG
                     KAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAEKTVVVSRLRRLVTQ"
     gene            complement(3617..4270)
                     /gene="fdnI"
                     /locus_tag="OO357_003221"
     CDS             complement(3617..4270)
                     /gene="fdnI"
                     /locus_tag="OO357_003221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit gamma"
                     /protein_id="HCR5888062.1"
                     /translation="MSKSKMIVRTKFIDRACHWTVVICFFLVALSGISFFFPTLQWLT
                     QTFGTPQMGRILHPFFGIAIFVALMFMFVRFVHHNIPDKKDIPWLLNIVEVLKGNEHK
                     VADVGKYNAGQKMMFWSIMSMIFVLLVTGVIIWRPYFAQYFPMQVVRYSLLIHAAAGI
                     ILIHAILIHMYMAFWVKGSIKGMIEGKVSRRWAKKHHPRWYREIEKAEAKKESEEGI"
     gene            complement(4263..5147)
                     /gene="fdnH"
                     /locus_tag="OO357_003222"
     CDS             complement(4263..5147)
                     /gene="fdnH"
                     /locus_tag="OO357_003222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase N subunit beta"
                     /protein_id="HCR5888063.1"
                     /translation="MAMETQDIIKRSATNSITPPSQVRDYKAEVAKLIDVSTCIGCKA
                     CQVACSEWNDIRDEVGHCVGVYDNPADLSAKSWTVMRFSETEQNGKLEWLIRKDGCMH
                     CEDPGCLKACPSAGAIIQYANGIVDFQSENCIGCGYCIAGCPFNIPRLNKEDNRVYKC
                     TLCVDRVSVGQEPACVKTCPTGAIHFGTKKEMLELAEQRVAKLKARGYEHAGVYNPEG
                     VGGTHVMYVLHHADQPELYHGLPKDPKIDTSVSLWKGALKPLAAAGFIATFAGLIFHY
                     IGIGPNKEVDDDEEDHHE"
     gene            complement(5160..8207)
                     /gene="fdnG"
                     /locus_tag="OO357_003223"
     CDS             complement(5160..8207)
                     /gene="fdnG"
                     /locus_tag="OO357_003223"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7620..7622),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5888064.1"
                     /translation="MDVSRRQFFKICAGGMAGTTVAALGFAPKQALAQARNYKLLRAK
                     EIRNTCTYCSVGCGLLMYSLGDGAKNAREAIYHIEGDPDHPVSRGALCPKGAGLLDYV
                     NSENRLRYPKYRAPGSDKWQRISWEEAFSRIAKLMKADRDANFIEKNEQGVTVNRWLS
                     TGMLCASGASNETGMLTQKFARSLGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANVVMVMGGNAAEAHPVGFRWAMEAKNNNDATLIVVDPRFTRTASVADIYAPIR
                     SGTDITFLSGILRYLIENNKINAEYVKHYTNASLLVRDDFAFEDGLFSGYDAEKRQYD
                     KSSWNYQFDENGYAKRDETLTHPRCVWNLLKAHVSRYTPDVVENICGTPKADFLKVCE
                     VLASTSAPDRTTTFLYALGWTQHTVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSTSLPGYLTLPSEKQVDLQSYLEANTPKATLADQVNYWSNYPKFFVSL
                     MKSFYGDAAQKENNWGYDWLPKWDQTYDVIKYFNMMDEGKVTGYFCQGFNPVASFPDK
                     NKVVSCLSKLKYMVVIDPLVTETSTFWQNHGESNDVDPASIQTEVFRLPSTCFAEEDG
                     SIANSGRWLQWHWKGQDAPGEARNDGEILAGIYHHLRELYQAEGGKGVEPLMKMSWNY
                     KQPHEPQSDEVAKENNGYALEDLYDANGVLIAKKGQLLSSFAHLRDDGTTASSCWIYT
                     GSWTEQGNQMANRDNSDPSGLGNTLGWAWAWPLNRRVLYNRASADINGKPWDPKRMLI
                     QWNGSKWTGNDIPDFGNAAPGTPTGPFIMQPEGMGRLFAINKMAEGPFPEHYEPIETP
                     LGTNPLHPNVVSNPVVRLYEQDALRMGKKEQFPYVGTTYRLTEHFHTWTKHALLNAIA
                     QPEQFVEISETQAAAKGINNGDRVTVSSKRGFIRAVAVVTRRLKPLNVNGQQVETVGI
                     PIHWGFEGVARKGYIANTLTPNVGDANSQTPEYKAFLVNIEKA"
     gene            8439..9320
                     /gene="yddG"
                     /locus_tag="OO357_003224"
     CDS             8439..9320
                     /gene="yddG"
                     /locus_tag="OO357_003224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid efflux DMT transporter YddG"
                     /protein_id="HCR5888065.1"
                     /translation="MTRQKATLIGLIAIVLWSTMVGLIRGVSEGLGPVGGAAAIYSLS
                     GLLLIFTVGFPRIRQIPKGYLLAGSLLFVSYEIYLALSLGYAATRHQAIEVGMVNYLW
                     PSLTILFAILFNGQKTNWLIVPGLLLALVGVCWVLGGENGLHYDEIINNITTSPLSYF
                     LAFIGAFIWAAYCTVTNKYARGFNGITVFVLLTGACLWIYYFLTPQPEMVFSTPVMIK
                     LISAAFTLGFAYTAWNVGILHGNVTIMAVGSYFTPVLSSALAAVLLSAPLSFSFWQGA
                     LMVCGGSLLCWLATRRG"
     gene            9575..10675
                     /gene="ompC"
                     /locus_tag="OO357_003225"
     CDS             9575..10675
                     /gene="ompC"
                     /locus_tag="OO357_003225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpC"
                     /protein_id="HCR5888066.1"
                     /translation="MKLKIVAVVVTGLLAANVAHAAEVYNKDGNKLDLYGKVTALRYF
                     TDDKRDDGDKTYARLGFKGETQINDQMIGFGHWEYDFKGYNDEANGSRGNKTRLAYAG
                     LKISEFGSLDYGRNYGVGYDIGSWTDMLPEFGGDTWSQKDVFMTYRTTGLATYRNYDF
                     FGLIEGLNFAAQYQGKNKRTDNSHLYGADDTRANGDGFGISSTYVYDGFGIGAVYTKS
                     DRTNAQERAAANPLNASGKNAELWATGIKYDANNIYFAANYDETLNMTTYGDGYISNK
                     AQSFEVVAQYQFDFGLRPSLAYLKSKGRDLGRYADQDMIEYIDVGATYFFNKNMSTYV
                     DYKINLIDESDFTRAVDIRTDNIVATGITYQF"
     gene            10839..11324
                     /locus_tag="OO357_003226"
     CDS             10839..11324
                     /locus_tag="OO357_003226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5888067.1"
                     /translation="MIMTNINTACVKNNASYQLNNALPNKETISSNFCERLEQWGNKS
                     VNNGEERAIAVERIKEAYNSNMASLDLSYLDLSELPPIPSTVNTLNLENNCLTCLDLA
                     DNASLVNINLSVNKINTITFPKEPKLENIYINHNNLESLDLKNQHSLVNLEAQNNSLK
                     K"
     gene            11434..12423
                     /locus_tag="OO357_003227"
     CDS             11434..12423
                     /locus_tag="OO357_003227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine-rich repeat domain-containing protein"
                     /protein_id="HCR5888068.1"
                     /translation="MITDLILHNHPRMKTITLNDNHIAHLNAKNTTKLEYLNLSNNNL
                     LPTNDIDQLISSKHLWHVLVNGINNDPLAQMQYWTAVRNIIDDTNEVTIDLSGLNLTT
                     QPPGLQNFTSINLDNNQLTHFDATNYDRLVKLSLNSNALESINFPQGRNVSITHISMN
                     NNALRNIDIDRLSSVTYFSAAHNQLEFVQLESCEWLQYLNLSHNQLTDIVAGNKNELL
                     LLDLSHNKLTSLHNVLFPNLNTLLINNNLLSEIKIFYSNFRNVQTLNAANNQLKYINL
                     DFLTYLPSIKSLRLDNNKITHIDTNNTSDIGTLFPIIKQSKNLNFLNVSGKNN"
BASE COUNT         3336 a         3126 c         2940 g         3250 t
ORIGIN      
        1 accattgcct gcgcaatggt gtttttatct gctttatact tggggacgac gccctggcgg
       61 taaagcaaag acgataaaag cgtgccaggg atggatattc aaaaaagagt gagtgacatg
      121 gaaccaaaaa caaaaaaaca gcgttcgctt tatatccctt acgctggccc tgtactgctg
      181 gaatttccgt tgttgaataa aggcagtgcc ttcagcatgg aagaacgccg taacttcaac
      241 ctgctggggt tactgccgga agtggtcgaa accatcgaag aacaagcgga acgagcatgg
      301 atccagtatc agggattcaa aaccgaaatc gacaaacaca tctacctgcg taacatccag
      361 gacaccaacg aaaccctctt ctaccgtctg gtaaacaatc atcttgatga gatgatgcct
      421 gttatttata ccccaaccgt cggcgcagcc tgtgagcgtt tttctgagat ctaccgccgt
      481 tcacgcggcg tgtttatctc ttaccagaac cgccacaata tggacgatat tctgcaaaac
      541 gtgccgaacc ataatattaa agtgattgtg gtgactgacg gtgaacgtat tctggggctt
      601 ggtgaccagg gcatcggcgg gatgggcatt ccgatcggta aactgtcgct ctataccgcc
      661 tgtggcggca tcagcccggc gtataccctt ccggtggtgc tggatgtcgg aacgaacaac
      721 caacagctgc ttaacgatcc gctgtatatg ggctggcgta atccgcgtat cactgacgat
      781 gaatactatg aattcgttga tgaatttatc caggctgtga aacaacgctg gccggacgtg
      841 ctgttgcagt ttgaagactt tgctcaaaaa aatgcgatgc cgttacttaa ccgctatcgc
      901 aatgaaattt gttcttttaa cgatgacatt cagggcactg cggcggtaac agtcggcaca
      961 ctgatcgcag ccagccgcgc ggcaggtggt cagttaagcg agaaaaaaat cgtcttcctt
     1021 ggcgcaggtt cagcgggatg cggcattgcc gaaatgatca tcgcccagac ccagcgcgaa
     1081 ggattaagcg aggaagcagc gcggcagaaa gtctttatgg tcgatcgctt tggcctgctg
     1141 acggacaaga tgccgaacct gctgcctttc cagaccaaac tggtgcagaa gcgcgaaaac
     1201 ctcagtgact gggataccga cagcgatgtg ttgtcactgc tggatgtggt gcgcaatgta
     1261 aaaccagata ttctgattgg cgtctcagga cagaccgggc tgtttacgga agagatcatc
     1321 cgtgagatgc ataaacactg tccgcgtccg atcgtgatgc cgctgtctaa cccgacgtct
     1381 cgcgtggaag ccacaccgca ggacattatc gcctggaccg aaggtaacgc gctggtcgcc
     1441 actggcagcc cgtttaatcc tgtggtatgg aaagataaaa tctaccctat cgcccagtgt
     1501 aacaacgcct ttattttccc gggcatcggc ctgggtgtta ttgcttccgg cgcgtcacgt
     1561 atcaccgatg agatgctgat gtcggcaagt gaaacgcttg ctcagtattc gccgctggtc
     1621 ctgaacggcg aaggtctggt actaccggaa ctgaaagata ttcagaaagt ctcccgcgca
     1681 attgcgtttg cggttggcaa aatggcgcag cagcaaggcg tggcggtcaa aacgtctgct
     1741 gaagctttgc aacaagccat tgacgataat ttctggcaag ccgaataccg cgactaccgc
     1801 cgtacctcca tctaagcctg cacccggtag tgaaggctac cgggctattt ccctctccct
     1861 ttttcagatc tcatccatac tgggtagtgg cgaataaatc tcatttgcct cacctgctat
     1921 gcagaacatc atccgaaaag gaggaactat gaagactgca gttgttacga aggatcatca
     1981 tgttgacgtt acggataaaa cactgcgctc actgaaacat ggcgaagccc tgctgaaaat
     2041 ggagtgttgt ggtgtatgtc ataccgatct tcatgttaag aatggcgatt ttggtgacaa
     2101 aaccggcgta attctgggcc atgaaggcat cggtgtggtg gcagaagttg gcccaggtgt
     2161 cacctcatta aaaccaggcg atcgtgccag cgtggcgtgg ttttacgaag gatgcggtca
     2221 ttgcgaatac tgtaacagtg gtaacgaaac gctctgccgt tcagttaaaa atgccggata
     2281 cagcgttgat ggcgggatgg aggaagagtg catcgtggtc gccgattacg cggtaaaagt
     2341 gccagatggt ctggactcgg cggcgcccag cagcattacc tgtgcggggg tcaccaccta
     2401 caaagccgtt aaactgtcaa aaattcgtcc cggtcagtgg attgctatct acggtcttgg
     2461 cggtctgggt aacctcgccc tgcaatacgc aaagaatgtc tttaacgcca aagtgatcgc
     2521 cattgatgtc aatgatgagc agttaaaact ggcaaccgaa atggatgcag atttagcgat
     2581 taactcacgc accgaagacg ccgccaaaat tgtgcaggag aaaaccggtg gcgctcacgc
     2641 tgcggtggta acagcagtag ctaaagctgc gtttaactcg gcagttgatg ctgtccgtgc
     2701 aggcggtcgt gttgtggctg tcggtctgcc gccggagtct atgagcctgg atatcccacg
     2761 tcttgtgctg gatggcattg aagtggtcgg ttcgctggtc ggcacgcgcc aggatctaac
     2821 cgaagccttc cagtttgccg ccgaaggcaa agtggtgccg aaagtcgccc tgcgtccgtt
     2881 agcggacatc aacaccatct ttaccgagat ggaagaaggc aaaatccgtg gccgtatggt
     2941 gattgatttc cgtcactaag aggtctttgc tgcgactccc atgttcgggt cgcagcatcg
     3001 cacactctcc aacatgaaat ggctaaatgg attattgttg cgtaggaagg aacaataatg
     3061 atagtgttcc cagagaagta acgctactga gagtaaagac actaatgaaa atggccaatc
     3121 atccccgccc gggggacatt attcaggaat cactggacga acttaatgtc agcctgcgcg
     3181 agtttgccag agcaatggaa attgcgccct caacggcaag ccgattgctg accggaaaag
     3241 cagctttgac gccagaaatg gcaattaaac tttccgtggt gatcggcagt tcgccgcaaa
     3301 tgtggctgaa tctgcaaaac gcctggagtc tggcagaggc agaaaaaacg gtggttgtgt
     3361 cgcgattacg ccgtctggtt acgcaataaa aaatggcacc ctataaagcg ccatttgaaa
     3421 ctgatgacaa acgcaacatt gcctgatgcg ctacgcttat caggcctaca cgttcacttc
     3481 aatctattgt atttgaaggg ttttcatgcc ggataaggca ttcacgacgc atccgggatg
     3541 aacaaagccc acgttgtcta caatatgaaa atggcgcctt ttacagcgcc attttcagct
     3601 taaagttcaa aagagattat atcccttctt cactctcttt tttcgcttct gccttctcga
     3661 tttcacgata ccagcgcgga tggtgtttct tcgcccagcg acggctcacc ttcccttcga
     3721 tcatcccttt aatcgaccct ttcacccaga atgccatata catatggatc aggatggcgt
     3781 ggatcaggat gatacccgca gccgcgtgga tcagcaggct gtagcgaaca acctgcatcg
     3841 ggaagtactg cgcaaagtac ggacgccaga taatcacccc ggtcaccagc agcacgaaaa
     3901 tcatgctcat gatcgaccag aacatcatct tttgcccggc gttgtactta ccaacatccg
     3961 ccactttgtg ctcattgcct tttaatactt cgacaatgtt caacagccac ggaatatctt
     4021 tcttatccgg gatgttgtga tgcacaaaac gcacaaacat aaacatcagt gcgacgaaaa
     4081 tcgcaatgcc gaagaagggg tgcaaaatgc gtcccatctg cggcgtaccg aaggtttgcg
     4141 tcagccattg cagcgtcggg aagaagaacg aaatcccgga cagcgccacc aggaagaagc
     4201 aaatcaccac ggtccagtga caggcgcgat caataaattt ggtgcgcaca atcattttcg
     4261 acttactcat gatgatcctc ctcgtcatcg tccacttcct tattcgggcc aataccgatg
     4321 tagtggaaaa tcaacccggc aaaagtggca ataaagccag ccgctgccag cggtttcaac
     4381 gcgcctttcc acaggcttac cgaggtgtcg atcttcggat ctttcggcag accgtgatac
     4441 agctccggct gatcggcgtg atgcagcacg tacataacgt gcgtaccacc gaccccttcc
     4501 gggttgtaga cgccagcatg ttcgtaacca cgcgctttca gtttcgccac gcgctgttcc
     4561 gccagctcca gcatctcctt cttggtgccg aagtggatag ccccggtcgg acaggttttc
     4621 acacaagccg gttcctggcc gacgctgacg cgatcgacgc agagcgtgca tttatatacc
     4681 cggttatcct ctttgttgag gcgcggaata ttaaacggac acccggcaat gcagtaacca
     4741 cagccgatgc agttttccga ctggaaatcg acaatcccgt tagcgtactg aatgattgca
     4801 ccagcagacg ggcacgcctt caggcagccc ggatcttcac agtgcataca gccgtcttta
     4861 cggatcagcc actccagctt gccattctgt tcggtttcgc taaagcgcat caccgtccag
     4921 gacttggcgc tcagatcggc ggggttatcg taaaccccga cgcagtgccc cacttcatca
     4981 cggatgtcgt tccactccga acacgccacc tgacaggctt tacagccgat acaggtggaa
     5041 acgtcgataa gtttcgcgac ttctgctttg taatcacgca cctgagaagg cggcgtgatg
     5101 gagttagttg cggacctttt gataatatcc tgcgtttcca tagccatctg ttcgccccct
     5161 tacgccttct cgatgttgac taagaacgct ttatattccg gcgtttgcga gtttgcatca
     5221 ccgacattcg gcgtcagagt gttagcgata taacctttac gcgcgacacc ctcaaagccc
     5281 cagtggattg gaatacccac cgtttcaacc tgctgaccgt ttacattcag cggtttcaga
     5341 cggcgcgtta ccactgccac cgcgcggata aagcctcgct tgctggagac agtgacacga
     5401 tcgccattat taatgccttt cgccgccgcc tgcgtttcgc tgatttccac aaactgttcc
     5461 ggctgagcaa ttgcgttgag caacgcgtgc ttggtccagg tgtggaagtg ctcggtcaga
     5521 cgataggtcg tacccacata cgggaactgc tcttttttac ccatccgcag cgcgtcttgt
     5581 tcatacagac gaacaaccgg gttagacacc acgttcggat gcagcgggtt agtgcccagc
     5641 ggcgtttcaa tcggctcgta gtgttccggg aacggacctt ccgccatttt gttgatggca
     5701 aacaggcgtc ccatcccttc cggctgcata ataaacggcc cggttggcgt acccggtgcg
     5761 gcattgccga agtccggaat atcgttaccc gtccacttgc tgccgttcca ctggatcagc
     5821 atccgtttcg gatcccacgg tttaccgttg atatccgccg aagcacggtt gtagagtacg
     5881 cgacggttga gcggccacgc ccaggcccat cccagcgtat tccccagacc ggacgggtcg
     5941 gagttatcgc ggtttgccat ctggttaccc tgctctgtcc agctaccggt gtagatccag
     6001 caagaagatg cggttgtacc gtcatcacgc agatgcgcaa agctactcag caactgacct
     6061 ttcttcgcaa tcagcacgcc attagcatca tagagatctt ccaacgcata gccgttgttc
     6121 tctttagcta cttcgtcaga ttgcggttcg tgcggctgct tgtagttcca gctcatcttc
     6181 atcagcggtt ctacgccttt accaccttcg gcctggtaca actcgcgcag atgatggtag
     6241 atacccgcca gaatttcgcc gtcgttacgc gcttcgcccg gcgcgtcctg acctttccag
     6301 tgccactgca accagcgacc ggagttagcg atagaaccat cctcttcagc aaagcaggtc
     6361 gaaggcagac ggaatacttc agtctgaata gacgccggat cgacatcgtt cgactcgccg
     6421 tggttctgcc agaaggtaga ggtttcagtc accagcggat cgataaccac catgtacttc
     6481 agtttgctca ggcagctcac cactttgttt ttatccggga aggacgcaac cgggttaaag
     6541 ccctggcaga aataaccggt aactttgcct tcatccatca tgttgaaata cttgatgacg
     6601 tcgtaggtct ggtcccactt cggcagccag tcatagcccc agttgttctc tttctgcgcg
     6661 gcatcgccat agaaagattt catcaggcta acgaagaact tcggatagtt gctccagtag
     6721 ttcacctgat cagccagcgt cgctttcggc gtgttcgctt ccagatacga ctgcaaatca
     6781 acctgttttt ctgacggcag cgtcagataa cctggcaggc tggtagagag cagacctaag
     6841 tcagtcagcc cctgaatgtt ggagtgacca cgcaatgcgt tcacgccgcc accggccata
     6901 cccatgttac cgagcagcaa ctggatcatc gccatagtac ggatgttctg cgcacccacg
     6961 gtgtgctgcg tccagcccag cgcgtacagg aaggtggttg tgcgatccgg tgcgctggtg
     7021 gaggccagca cttcacacac tttcaggaag tcggcttttg gcgtaccaca gatgttttca
     7081 acaacgtccg gcgtgtagcg ggaaacgtgc gctttcagca ggttccacac acagcgcgga
     7141 tgagtcagtg tttcatcgcg tttcgcatag ccgttttcat cgaactgata gttccaggac
     7201 gatttgtcgt actggcgttt ttcagcgtca taaccgctga acaggccatc ttcgaaagca
     7261 aaatcatcac gcaccagtag gctggcgttg gtgtaatgtt taacgtattc ggcgttgatt
     7321 ttgttgtttt cgatcaggta gcgcaaaatg ccagacagga acgtaatgtc cgtaccggaa
     7381 cgaataggtg cgtaaatatc cgccacagaa gcggtacgcg taaaacgggg atcgacaacg
     7441 atcaaggttg catcgttgtt gtttttcgct tccatcgccc agcggaaacc gacgggatgc
     7501 gcttcagcag cgttaccgcc cattaccatt acgacgttag cgtttttgat atccacccag
     7561 tggttggtca tagcaccgcg accaaatgtt ggagcaagac ttgctaccgt tggtccgtgt
     7621 cagacgcgcg cctggttgtc taccgccagc atcccgaggg agcgggcaaa tttctgggtc
     7681 agcatcccgg tttcgttgct ggcaccggag gcacacagca taccggtaga aagccaacgg
     7741 tttaccgtta cgccctgctc gttcttttca ataaagttag cgtcacggtc agctttcatc
     7801 agcttcgcaa tacgggagaa tgcttcttcc cagctaatgc gctgccattt gtcagaacct
     7861 ggcgcacgat atttcgggta gcgcagacgg ttttcactgt tgacgtaatc cagcaaaccg
     7921 gcccctttcg ggcacagcgc accacggctt accggatgat ccgggtcacc ttcaatgtga
     7981 taaatcgctt ctctggcgtt ttttgcgcca tcacccaggc tatacatcaa tagcccgcaa
     8041 cctacggaac agtatgtgca ggtgttacgg atctctttag cgcgtaataa tttgtagttt
     8101 cgcgcctgag ccagtgcttg cttcggggca aagcccaatg ccgctaccgt tgttccagcc
     8161 ataccgcccg cgcagatttt aaaaaattgt ctgcgactga cgtccattgc tttcctcttt
     8221 tttcaggggt attactgcgc gaggaaacta acagtaaaat tctgaatctt tttgcgcgaa
     8281 atcaaaaaga acacgtgttt gccacaaaat aatccgcctg cgcaaattga cctacctcaa
     8341 tagcggtaga aaaacgcacc actgcctgac aggccagtta aaaaaatgct ataaaattca
     8401 gcttaatttt taacggcaag agagacaaaa cagcgagcat gacacgacaa aaagcaacgc
     8461 tcatagggct aatagcgatc gtcctgtgga gcacgatggt aggattgatt cgcggtgtca
     8521 gcgaggggct cggcccggtc ggcggcgcag ctgctatcta ttcattaagc gggctgctgt
     8581 taatcttcac ggttggattt ccgcgtattc ggcagattcc gaaaggctat ttactcgccg
     8641 ggagtctgtt attcgtcagc tatgaaatct atctggcgct ttccttaggg tatgcggcga
     8701 cccgtcatca ggcgattgaa gtgggtatgg tgaactatct gtggcccagc ctgacaattc
     8761 tctttgccat tctgtttaat ggtcagaaaa ccaactggct gattgtacca ggattattat
     8821 tagccctggt cggcgtctgc tgggtgttag gcggtgagaa tgggttacat tatgatgaaa
     8881 tcatcaataa tatcaccacc agcccattga gttatttcct ggcgtttatt ggtgcgttta
     8941 tctgggcagc ctattgcaca gtaacgaata aatacgcacg cggatttaac ggaattaccg
     9001 tttttgtcct gctaacggga gcatgtctgt ggatttacta ttttctcacg ccacaaccag
     9061 aaatggtatt tagcacgccc gtcatgatta aactcatctc tgcggcattt accttaggat
     9121 ttgcttatac tgcatggaat gtcggtatat tgcatggcaa tgtcaccata atggcggtag
     9181 gctcgtattt tacgcctgta ctttcctcag cgcttgcagc cgtgctgctc agcgccccgc
     9241 tgtcgttctc gttctggcaa ggcgcactaa tggtctgcgg cggttccctg ctctgctggc
     9301 tggcgacacg tcgtggttaa aataattgcc gggtctacgg cccggcagtt aatcagataa
     9361 attccgacaa tattaaaaaa taaatttcaa ataccagtaa ttcaccttat atctcatttc
     9421 gacttcgatc acatatcgat aaataatatt attctatttt tctcattgtt tttaatcacc
     9481 attctatttt aaaaacagta tgtcatttat aaatgacgcc gtttaaaatt cgtttagaaa
     9541 atacgtttaa cgataattaa aaggattctg ttaaatgaaa ttaaaaatag ttgcggtggt
     9601 tgtaactggt ttgttagctg cgaacgtagc acacgctgcc gaagtctata acaaggatgg
     9661 taataaactc gacctttatg gcaaggttac cgctctacgt tattttactg atgataagcg
     9721 tgacgatggt gataaaactt atgcccgtct cggctttaaa ggagaaacgc aaatcaatga
     9781 tcaaatgatt ggttttggtc actgggaata tgattttaaa ggctataacg atgaagccaa
     9841 cggctcgcgc ggcaacaaga cccgtctggc ctatgctggt ttaaaaatta gtgaatttgg
     9901 ctctctggac tatggtcgta actacggtgt cggctacgac attggttcat ggaccgatat
     9961 gttgccagaa tttggtggcg atacctggag tcagaaagat gtcttcatga cataccgtac
    10021 caccggtcta gcaacctatc gcaactacga tttctttggc ttaattgaag ggctgaactt
    10081 tgccgcgcaa tatcaaggca aaaataaacg tactgacaac agtcatcttt atggtgctga
    10141 cgacacgcgt gccaacggtg acggtttcgg tatctcctca acttatgttt atgatggctt
    10201 tggtatcggt gcggtatata ccaaatccga tcggacaaat gcgcaggaaa gagccgcagc
    10261 taatcctctc aatgcctccg gtaagaatgc agaactatgg gccacaggta taaagtatga
    10321 tgccaacaac atctactttg cagctaatta cgatgaaaca ttaaacatga ccacctatgg
    10381 ggatggttat atctctaaca aagcacaaag ttttgaagtg gtggcgcaat atcaattcga
    10441 cttcggcttg cgcccatcac tcgcttacct gaaatcgaaa ggcagagatc tgggccgcta
    10501 cgccgaccag gacatgattg agtatatcga cgttggtgcg acgtatttct tcaacaaaaa
    10561 tatgtcgacc tatgttgatt ataaaatcaa cctgattgat gaaagcgact ttacccgtgc
    10621 cgtagatatt cgcaccgata acatcgtcgc tacgggcatt acctatcagt tctaacgtcc
    10681 tctcctgacc agcccttctc tgggctggtt tttgatttaa gtgctgctgt atgactcctt
    10741 aagtaaaaat gagcaattct tgatatttct ggtcattcac ctcaattaat attattccat
    10801 atcctccggg gacatcttac tataatattt attgatgagt gattatgacc aatattaata
    10861 cagcttgtgt aaaaaataat gccagttatc aacttaataa cgcattacct aacaaagaaa
    10921 ccatctccag caatttttgt gaacgactag aacaatgggg taataaatcg gttaataatg
    10981 gtgaagaaag agcaattgcc gtggagcgaa ttaaagaagc ttacaattcg aacatggcat
    11041 ctcttgattt gtcatatctt gatttgagtg aattaccgcc tatcccctcg acagttaata
    11101 cgttaaatct ggaaaacaat tgtctaactt gtcttgactt agctgacaat gccagtctcg
    11161 ttaatatcaa cctcagcgtt aataaaatta acacgataac ctttccaaaa gaaccaaaac
    11221 tggaaaacat ttatattaat cataacaatc tggaaagttt agatttaaaa aatcagcatt
    11281 cattggttaa tctggaagcg caaaataata gcctaaaaaa atgatatttc tgatagttat
    11341 aaactgaaat ttcttaatct tgattataac aagctagcat cgctggatgt ctcccggcaa
    11401 gaatctctga ttgagttaag tgcccatcac aatatgatca ctgaccttat attacacaat
    11461 catcccagga tgaaaacaat cactttaaac gacaaccata ttgcacattt aaacgccaaa
    11521 aacactacaa aactggaata tttaaactta agcaataaca atttactgcc aaccaatgac
    11581 attgatcaac taatatcatc aaaacatctt tggcatgtat tagttaacgg catcaacaat
    11641 gatccacttg cacaaatgca gtactggact gcagtaagaa atataattga tgacactaat
    11701 gaagtgacca ttgatttatc aggacttaat ttaaccactc aaccaccagg gctgcaaaac
    11761 ttcacctcta tcaatcttga taataaccaa ctcacacatt ttgatgcaac caactacgat
    11821 agactcgtaa agctaagtct gaatagtaat gctcttgagt caataaattt tcctcaaggc
    11881 agaaatgtaa gtattacaca tatatctatg aataataatg ctctcagaaa tattgatata
    11941 gataggcttt catcagttac ttattttagt gcggcacata atcaactaga gtttgtgcaa
    12001 ttagaatctt gcgaatggct gcaatacctg aatctcagcc ataatcaatt aactgatatt
    12061 gttgcaggta ataaaaatga actcttactg ctggatctat cccataataa actaacaagt
    12121 ttacacaatg tcttatttcc caacttgaat acgttactta ttaacaacaa tttgctttct
    12181 gaaattaaaa tattctatag caacttccgc aatgttcaga cattaaacgc tgctaacaac
    12241 cagttgaaat atataaatct tgatttcctg acttatcttc catctatcaa aagtttaaga
    12301 ctggacaata ataaaataac ccacattgat actaataata catccgatat tggaacttta
    12361 ttccccataa taaaacagag caaaaactta aattttttaa atgtttctgg gaagaacaat
    12421 tgacctacta tgcagctcat gttatttaat ttgttttccc cagcacttaa gcttaatact
    12481 ggcctggcaa ttctttcgcc tggtgcattt gaagttcact ctgacggaat agatgcggat
    12541 aacgaattgt ttcactatac tattaaaaaa gcatataccc catataatat acacacttac
    12601 aagacagagg aagttgtaaa ccagaggaat ataaaagtta aaaacatgac ct
//
LOCUS       DAJTRC010000102        12549 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.102, whole genome shotgun sequence.
ACCESSION   DAJTRC010000102 DAJTRC010000000
VERSION     DAJTRC010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12549
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.102"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..1040
                     /locus_tag="OO357_003228"
     CDS             <1..1040
                     /locus_tag="OO357_003228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000417858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HCR5888069.1"
                     /translation="FFPNLVDHLLVKHHTADGVEDGISICYFKPYSQGRARWQGDTIH
                     GVWFDEEPPYSIYGEGLTRTNKYGQFSILTFTPLMGMSDVVTKFLKNPSKSQKVVNMT
                     IYDAEHYTDEQKEQIIASYPEHEREARARGIPTMGSGRIFQIPEETIKCQPFECPDHF
                     YVIGGMDFGWDHPQAQVQLWWDKEADTIYVSRVWKAKEKTAVQAWGAVKSWAHKVPTA
                     WPHDGNQHEKGGGEQLKGQYADAGFMMLQEHATWPDGGNAVEPGITELRDMMLDGRFK
                     VFNTCEPFFEEFRLYHRDENGKIVKLNDDVLSAVRYAYMMRRFAKMMRDIKKPKEKKI
                     PAPIRPIARRT"
     gene            1041..3206
                     /locus_tag="OO357_003229"
     CDS             1041..3206
                     /locus_tag="OO357_003229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706171.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HCR5888070.1"
                     /translation="MADENRLNSILCKFDADWMASDEARTEATNDLYFSRVSQWDDWL
                     SNYTTLQYRGQFDVVRPEVRKLVAEMRRNPVDVLFRPKDGANPDAADVLMGMYRTDMR
                     HNTAKIAVNVGVREQIESGVGAWRLVTQYEDNDPTSNNQVIRRLPIHEACSHVIWDAN
                     SKQMDKSDAKHCTVINALSRNGWKEFAEDYGIDPDTLPSFQNPNDTWLFPWVSNDVVY
                     VAEYYEVEEEKEKVFIYRDPLTGEPVSYYQQDIKDVIDDLANRGFIKVAERKVKRRRV
                     YKSIITCTQILKDREKIAGEHIPIVPVYGEWSFAGDKECYEGVVRLTKDGQRLRNMIM
                     SFNADIVARSPKKKPTFFPEQIEGYEYMYGGNDDYPYYLQNRTDENGNDLPIGPISYM
                     ENPEVPQANAYMLEAATNAVKEVASLGVDAQAANGQVAFDTVNQLNMRADLETYVFQD
                     NLATAMRRDGEIYASMVNDIYDVPRHVTLTLEDGSEKDVQLYAQVVDYQSGNVVTLND
                     IRGRYECYTDVGPSFQSMKEQNRAEIQELLTKVPQGTPEFQMLMLQYFTLLDGKGVEM
                     MREYANKQLVMMGLKKPETPEEMEMVQQAQQQPQQPSAEQIQAQGILLQGQAELLKAE
                     NQQAQIQVEAAKVEAQNQLNAAKIAEIFNNMDLDKQAELREYLRLVGQFQQQRSKDAR
                     ANAELLLKDADQTHSQRMDFANLMRQVQIPSGGVAETPQ"
     gene            3220..4131
                     /locus_tag="OO357_003230"
     CDS             3220..4131
                     /locus_tag="OO357_003230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="scaffolding protein"
                     /protein_id="HCR5888071.1"
                     /translation="MDQTTDIQASEELTLPGNHAAASADGLVVDNANDSAGQEEGFEI
                     VRKDDEKPKQDPATNAEFARRRIERKRQRELEQQMEAVKRGELPEHLRVNPELPKQPD
                     PNDYLSEDALAKYDYDQSRALAAFQQANSEWQIKAMDARSQAVAEQGRKTQEFTQQSA
                     QYVEAARKHYEAAEKLNIPDYQEKEDAFMQLVPPAVGADIMRLFPEKSAALMYHLGAN
                     PEKTRQLLAMDGQSALIELTRLSERLTLKPRAKPVSEAPLPDEPIQGHAVAANISAIE
                     KQMEAAANKGDVETYRKLKAQLNKGIR"
     gene            4131..5426
                     /locus_tag="OO357_003231"
     CDS             4131..5426
                     /locus_tag="OO357_003231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coat protein"
                     /protein_id="HCR5888072.1"
                     /translation="MALNEGQLVTYALDEIIGTVQNLTPMASKVTKYPPPAESMQRSS
                     NTVWMPVEQEAPTQTGWDLTGNATGILELSVKCNMGDPDNDFFELRADDLRDERSYRR
                     RIQASAKKLANNIESAIAKQATEMGSLVVHDTRAIGPSTGLPGWDFVSDAERLMFSRE
                     LNRDMGISYFLNPDDYRKAGRNLVDGDIFGRVPEEAYRNGTIQRQIAGFDEILRSPKL
                     PAVTKSTATGVTVSGAQKFKPQAYTLDTDGNKENVDNRVATVTVSSTTGFKRGDKISF
                     IGVKFLSQMAKNVLTDDATFSITRVIDGTHIEITPKPIALDDASLTKEEKAYANVNTS
                     LADTTPVNVLNVATTTANVFWADDSIRLLSQPIPVTHELFAGMKTSSFSIPGIGVNGI
                     FATQGDINTLSGKCRIAVWYSACAVRPEAIGVGLPNQTA"
     gene            5471..5701
                     /locus_tag="OO357_003232"
     CDS             5471..5701
                     /locus_tag="OO357_003232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706174.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888073.1"
                     /translation="MTHMIFRHGDMKKWKGVGYDFEIVKAEELQEYLDAGWFSHPDDL
                     LKDVAEPEPEEKQRKKPGRKPKAAADEPDNEG"
     gene            5679..6179
                     /locus_tag="OO357_003233"
     CDS             5679..6179
                     /locus_tag="OO357_003233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RmuC"
                     /protein_id="HCR5888074.1"
                     /translation="MNLTTKGDLVLAALRKLGVASNATLTDVEPQSMEDGVNDLEMMM
                     AEWLGGDASPGINVGYIFADADVAPDPGDEHGLSNNAINAVIFNLACRIAPDYALEAS
                     AKLITTARYGKERLVKLSAMDRAKAAKCKSGYPNRMPVGSGNQLAKWNGWNYFHRKEP
                     CDNGSE"
     gene            6179..7597
                     /locus_tag="OO357_003234"
     CDS             6179..7597
                     /locus_tag="OO357_003234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="packaged DNA stabilization protein gp10"
                     /protein_id="HCR5888075.1"
                     /translation="MPIQQLPLMKGVGKDFRNADYIDYLPVNMLATPKEILNSSGYLR
                     SFPGIAKRSDVNGVSRGVEYNMAQNAVYRVCGGKLYKGESEVGDVAGSGRVSMAHGRT
                     SQAVGVNGQLVEYRYDGTVKTVSNWPTDSGFTQYELGSVRDITRLRGRYAWSKDGTDS
                     WFITDLEDESHPDRYSAQYRAESQPDGIIGIGTWRDFIVCFGSSTIEYFSLTGATTVG
                     AALYVAQPSLMVQKGIAGTYCKTPFADSYAFISNPATGAPSVYIIDSGQVSPIASASI
                     EKILRSYTADELADGVMESLRFDAHELLIIHLPRHVLVYDASSIANGPQWCVLKTGLY
                     DDVYRAIDFVYEGNQITCGDKLESVTGKLQFDISSQYDKQQEHLLFTPLFKADNARVF
                     DLEVESSTGVAQYADRLFLSATTDCINYGREQMIEQNEPFVYDKRVLWKRVGRIRKNV
                     GFKLRVITKSPVTLSGAQIRIE"
     gene            7597..8445
                     /locus_tag="OO357_003235"
     CDS             7597..8445
                     /locus_tag="OO357_003235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888076.1"
                     /translation="MADSNLNVPVIIQATRLDTSVLPRNIFSQSYLLYVIAQGTDVGN
                     VANKANEAGQGAYDAQVRNDEQDVILADHEQRISAAEATLVNHEERIRQAESTLQDHE
                     TRIAQNESDISSLDTRVQSLESQVSDHETRIDALEYATTRKKSEVVYSGVSVTIPTAP
                     TNLVSLLKTLTPSSGSLAPFFDTVNNKMVVFNENKTLLFKLSIVGTWPSGTANRSMQL
                     TFSGSVPDTLVSSRNAATTTDNILLATFFSVDKDGFLATNGSTLTIQSNGAAFTATTI
                     KIIAEQ"
     gene            8445..8900
                     /locus_tag="OO357_003236"
     CDS             8445..8900
                     /locus_tag="OO357_003236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706178.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2824 family protein"
                     /protein_id="HCR5888077.1"
                     /translation="MITFKPTRNIDLIEAVGNHPDIIAGSNNGDGYDYKPECRYFEVN
                     VHGQFGGIVYYQEIQPLTLDCHAMYLPEIRGFSKEIGLAFWRYILTNTTVQCVTSFAA
                     RKFRHGQMYCAMIGLKRVGTIKKYFKGVDDVTFYSATREELIDFLNHGR"
     gene            8903..9595
                     /locus_tag="OO357_003237"
     CDS             8903..9595
                     /locus_tag="OO357_003237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA transfer protein"
                     /protein_id="HCR5888078.1"
                     /translation="MLYAFKLGRKLRGEEPYCPEKGGKGGSSDKSAKYAAEAQKYAAD
                     LQNQQWQTIMKNLAPFTPLAEQYVNQLQNLSSLEGQGQALNQYYNSKQYKDLAGQARY
                     QSLAAAEATGGLGSTATSNQLATIAPTLGQSWLSNQMSNYNNLANVGLGALQGQANAG
                     QTYANNMSSIAQQSAALAAANANKPSSLQTAISGGTSGAIAGAGLASLLGTSTPWGAG
                     IGAGIGLLGSLF"
     gene            9605..10936
                     /locus_tag="OO357_003238"
     CDS             9605..10936
                     /locus_tag="OO357_003238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage DNA ejection protein"
                     /protein_id="HCR5888079.1"
                     /translation="MATWQGTNGGLLAGIGGVNSNAPSVNDIGNTLQLIRQNNDIERS
                     GANNVGLTALQGLSGIAGVFQQEKQAQRQKEFQQAYANAYASGDRGALRQLATQYPDQ
                     IESVRKGMGFIDEEQRNSIGTLAAGARLASSSPEAMQSWLQNNAGELARVGVNPHDVA
                     QMYQQNPRQFGEFVDHLGMNSLGPEKYFDLQDKMQGRQVTMRGQDLDSQTAARNQAIT
                     MRGQDIQANLGQQRINLDAETNRINNENKRLDRMLSAETNDLKRQEIQSRIAANNQQL
                     QQKQQALNDGYKDGINTLTTSMFTLNDIVSSPSLKSITGLRGVIPNVPGSQAADTQAR
                     LDTFKSQAYLTAVQAMRGMGALSDAEGKKLDQAVGSLQNSQSEESFRRNAGVILNTLN
                     QKRNEAVGKYVQQNGIKRVEAPQASIDYLKQHPELSIDFINRYGYLPSLGQ"
     gene            10937..>12549
                     /locus_tag="OO357_003239"
     CDS             10937..>12549
                     /locus_tag="OO357_003239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HCR5888080.1"
                     /translation="MANYRDLLEQAGARYGVPEGLMTALGAKESSYNPAAVSSAGAVG
                     LTQVMPGTWRDMGYTDEQMQNPEYQADAGARYLAKMYQQFGNWRDSLQAYHDGPGNVM
                     KAKRGEYTPGPEGRGYVDDRFAQWAGDPVTDSTVEQRATSAKVHPQQDPNNPFAQLEV
                     QSSEQVSASGVQSDPNNPFAQIEQQAASQQPPQPVSSVAPKPVQQQTVNQANNAPTRE
                     EPSLMQQAGDWLTGGQSAGQIAEQAGRGLVNIPFDVLQGGASLINAISQGLGGPKVLD
                     DVYRPVDRPTDPYAQAGESIGGYLVPGAGVAGNMVIGSLADAANQRGDFAENAAINAG
                     LSIATHGLINGVTRGVRGASNIISGNKTSAQRATTAPTETSPFSGDAAAATNPAVHAA
                     EARVAQGVPMTPATKNPEEVVRTVAAQKRPNLASSLDELDINPQAEVLESAERLNVDS
                     LLPSHFSGNEQYKAVEQAIKSRAGSALQVQENEAIRQLAQGAGEIIDRVSGAKDALGM
                     SDKFIDTVNGRMSALMKRSDQLYRNVEKA"
BASE COUNT         3369 a         3108 c         3308 g         2764 t
ORIGIN      
        1 ctttctttcc gaaccttgtt gatcaccttc tggttaagca tcacacggct gatggcgttg
       61 aagatggcat ttcaatctgc tacttcaagc catactcgca aggccgtgct cgctggcagg
      121 gtgacacaat ccacggcgtg tggtttgacg aagagccacc atacagcatt tatggcgaag
      181 gtcttacccg taccaacaaa tacgggcaat tctcaattct gacgtttacc ccgctgatgg
      241 ggatgtctga cgttgttacc aagttcctga agaaccccag caagtcgcag aaagtggtca
      301 acatgaccat ctatgacgct gagcactata ccgacgagca gaaagagcaa atcatcgcat
      361 catatcccga gcatgagaga gaggcgcgtg ctcgcggtat tcctacgatg ggtagtggtc
      421 gaatcttcca gataccggaa gagacgatta agtgtcagcc gttcgagtgt cctgatcact
      481 tctacgtaat tggcgggatg gatttcggat gggatcaccc acaggcgcaa gttcagcttt
      541 ggtgggataa ggaggcagac acaatctacg tttcacgcgt gtggaaggcg aaagaaaaaa
      601 cagccgttca ggcgtgggga gccgttaaat catgggcgca taaagtgcca accgcatggc
      661 ctcatgatgg aaaccagcac gagaagggcg gcggtgagca gctcaaaggg cagtatgcag
      721 acgctggatt tatgatgttg caggagcatg cgacatggcc tgatggcggt aacgctgttg
      781 agccaggcat cactgaattg cgcgacatga tgctcgatgg tcgcttcaaa gtattcaata
      841 cctgtgagcc attctttgag gagttccgcc tctatcaccg tgatgaaaac gggaaaatcg
      901 tcaagcttaa cgacgacgtg ctatccgccg ttcgctatgc atacatgatg cgccgcttcg
      961 caaaaatgat gcgcgacatc aaaaaaccaa aagagaaaaa gataccagcc ccaatcaggc
     1021 ccatcgcacg gagaacttaa atggccgacg aaaacagact caattccatt ctgtgtaagt
     1081 ttgacgcaga ctggatggcg agcgatgaag ccagaaccga ggcgacaaat gacctgtatt
     1141 ttagccgagt gtcgcaatgg gatgactggc tatcaaacta cactaccctg caatatcgcg
     1201 gacaattcga tgttgttcgc ccggaggtca ggaaactggt cgcagagatg cgccggaacc
     1261 ctgtcgacgt tctcttccga cccaaagacg gcgctaatcc tgatgcagcc gatgtattga
     1321 tggggatgta tcgtactgat atgcgccata acacggcaaa gattgccgtt aacgttggcg
     1381 ttcgtgagca gatagagtca ggcgttggtg catggcgtct ggtcacgcag tacgaagaca
     1441 acgatccaac aagcaacaat caggtaatcc gacgcctgcc aatccatgaa gcctgctcac
     1501 acgtcatatg ggacgccaac agcaagcaga tggataagag cgacgctaag cactgcacgg
     1561 tgattaacgc cttgtcgcgc aatggctgga aagagttcgc agaggattac ggtattgacc
     1621 cggataccct gccatctttc cagaatccta acgatacatg gctattcccg tgggtgtcga
     1681 atgatgtcgt ctacgtcgct gagtattacg aggtagaaga ggagaaggag aaggtcttca
     1741 tctaccgcga cccgctgaca ggtgagccgg tcagctatta ccagcaggat atcaaagacg
     1801 tcatcgacga cctggctaat cgtggattca ttaaggtagc agagcgcaag gtgaagcgtc
     1861 ggcgtgtgta taagtcgatc atcacctgta cgcagatact gaaagaccgc gagaagatag
     1921 ccggagagca tattccaatc gttccagtgt atggcgaatg gtcattcgct ggtgacaagg
     1981 agtgctacga gggtgtggta aggctgacga aagacggtca acgccttcgt aacatgatta
     2041 tgtcgttcaa cgccgatatt gttgctcggt caccgaagaa gaaaccgacc ttcttccctg
     2101 agcaaatcga aggctacgaa tacatgtacg gcggaaatga tgactatccg tactatctgc
     2161 agaacaggac tgatgaaaac ggtaacgacc tgccgattgg tccaatctcc tacatggaaa
     2221 accctgaagt gccgcaagcc aacgcttaca tgcttgaggc tgccaccaac gcagtgaaag
     2281 aagtggctag tcttggcgtg gatgcgcagg cggcaaatgg tcaggtcgct tttgataccg
     2341 tcaatcaact gaacatgcgg gcagaccttg agacatacgt gtttcaggat aacctggcta
     2401 ccgcaatgcg acgtgatggc gagatttatg cctcaatggt caacgatatt tatgacgttc
     2461 ctcgtcatgt aacgctgaca cttgaagatg gaagcgagaa agacgttcaa ctctatgcgc
     2521 aagttgtcga ttaccagtcc ggcaatgtgg tcacactcaa cgacattcgc ggtcgatatg
     2581 agtgctatac agacgttggg ccatccttcc agagtatgaa ggaacagaac cgcgcagaga
     2641 ttcaggagtt gctcaccaag gttccgcaag gtactccaga gttccagatg ctgatgctgc
     2701 aatacttcac gctgcttgac ggtaaaggcg tcgagatgat gcgagagtac gcgaacaagc
     2761 aactggtgat gatggggctg aagaaaccag aaacacctga agagatggag atggtgcagc
     2821 aggcacaaca acagccgcag cagccatcag cagagcaaat tcaggcgcag ggtatccttc
     2881 tgcaaggtca ggctgaattg ctcaaggcag agaaccaaca ggcgcagatt caggttgaag
     2941 ctgccaaggt tgaagcccaa aaccaactca acgccgcgaa gattgcagaa atattcaaca
     3001 atatggacct cgacaagcag gcagaactgc gtgagtacct caggctcgta ggtcaattcc
     3061 agcaacagcg cagcaaagat gctcgtgcta acgctgagct gcttcttaaa gatgcagacc
     3121 agactcattc acaacgcatg gatttcgcga atcttatgcg tcaagttcaa atcccctccg
     3181 gcggagtagc cgagacacct caataagaga gagttaatca tggaccaaac caccgacatt
     3241 caggcttctg aagaattaac cctgcccggc aatcatgcag cggcatctgc tgatggctta
     3301 gttgtcgata atgccaacga cagcgcaggt caggaagaag gctttgagat tgtccggaaa
     3361 gacgatgaga aaccaaaaca agacccggca actaatgctg aatttgcccg tcgccgcatc
     3421 gaacgcaaac gccagcgtga gcttgagcag cagatggaag cggttaagcg tggagagttg
     3481 ccggagcacc tgcgggtgaa ccctgagtta ccaaaacaac cagaccctaa cgattatctt
     3541 tccgaagatg cactggctaa gtacgactat gaccagagcc gcgcactggc tgccttccag
     3601 caggcaaaca gtgaatggca gatcaaggct atggacgcac gaagccaggc tgtcgccgag
     3661 cagggtcgca aaactcagga gttcacccag caatcagcgc aatacgtcga ggcggcccgt
     3721 aagcactacg aggcagcgga aaagctcaat atccctgact atcaggagaa agaggatgca
     3781 ttcatgcaac tggtgccgcc agcagtcggt gccgacatca tgcgcctctt cccggagaaa
     3841 tccgccgctc tcatgtatca ccttggtgct aatcctgaga aaacacgcca gttgctggcg
     3901 atggacgggc aatccgcgct gattgaactc actcgactgt cagaacgttt aactctcaag
     3961 cctcgagcca agcctgtttc agaagccccg ttacctgatg aacccattca gggacatgct
     4021 gttgctgcaa atatctctgc gattgaaaag cagatggagg cggcagcaaa caaaggggat
     4081 gtagagacgt accgcaagct caaggcgcaa ctgaataaag gaattcgata atggcattaa
     4141 atgaaggtca actggtcacg tatgctctgg atgaaatcat cggaaccgtc cagaacctga
     4201 cgccaatggc gtcaaaagtg acaaaatacc cccctccggc agaatccatg cagcgttcaa
     4261 gcaacaccgt gtggatgcct gttgagcagg aagcgccaac ccagactggc tgggatttaa
     4321 ctggcaacgc aaccgggatt ctggaactct ccgtgaagtg caacatgggc gatccggata
     4381 acgatttctt cgagcttcgt gcagatgacc tgcgtgatga gcgttcttac cgtcgccgca
     4441 tccaggcatc cgccaaaaaa ctggcgaata acattgagtc agcgattgcc aaacaggcaa
     4501 ctgaaatggg ctcgcttgtt gttcacgata cccgcgcaat tggtccatcg actggcctgc
     4561 ctggctggga ttttgtgtct gatgcagagc gactgatgtt ctcccgtgag ctaaaccgcg
     4621 acatgggcat cagttacttc ctgaaccctg acgattaccg caaagcaggc cgcaacctgg
     4681 tagatggtga catcttcggg cgcgttcctg aagaagcata tcgaaacggt actattcagc
     4741 gtcagattgc tggctttgat gaaattcttc gctcaccgaa acttccggca gttaccaagt
     4801 caaccgcgac tggtgtaact gtttctggtg cgcagaagtt taagccgcag gcatacaccc
     4861 ttgataccga tggtaacaaa gagaacgtcg acaaccgtgt tgcaacggtg accgtatcct
     4921 ccaccaccgg atttaagcgc ggcgacaaaa tcagcttcat tggtgtgaag ttcctgtctc
     4981 agatggcgaa gaatgtgctg actgatgacg cgactttctc aatcacccgt gtgatcgatg
     5041 gtactcacat cgaaatcacg ccgaagccga ttgcgctgga tgacgcgtca ctgacaaaag
     5101 aagagaaggc ttacgctaac gtaaacacct ctcttgctga taccactccg gtaaacgttc
     5161 tgaacgtggc aacaaccacc gctaacgtgt tctgggctga tgactcaatc cgcctgctgt
     5221 ctcagccgat cccggtaacc catgaactgt ttgctggcat gaaaacgtct tccttcagca
     5281 ttccaggtat tggtgttaac ggcatcttcg caacgcaggg tgatatcaac actctgtctg
     5341 gtaagtgccg tattgctgtg tggtattcag catgtgctgt acgaccagag gcaattggtg
     5401 ttggtctgcc taaccagacc gcgtgataac cagagggagc ttcggctccc gtttttatct
     5461 ggagacaagc atgacacaca tgatctttcg tcatggcgac atgaagaagt ggaaaggcgt
     5521 tggctacgac tttgaaatcg tgaaagccga agagcttcag gaatatctgg atgctggctg
     5581 gttttcacat cctgatgacc ttttgaagga tgttgcagag ccagagccag aagaaaaaca
     5641 gcgtaaaaag cctggtcgaa aacctaaggc ggcagcagat gaacctgaca acgaagggtg
     5701 atttagttct tgcggcatta cgtaagctcg gtgttgcatc aaatgccacg ttaaccgatg
     5761 tcgaaccgca gtctatggaa gacggcgtca acgaccttga aatgatgatg gctgaatggc
     5821 ttggcggtga tgcgtcacct ggtatcaacg ttggctacat ttttgctgat gcagatgttg
     5881 ctccggatcc gggcgatgag cacggtttat caaataacgc tatcaatgcc gtcattttca
     5941 accttgcctg ccgaattgct ccagattatg cgctggaagc gtctgcaaaa cttataacca
     6001 ctgccagata cgggaaagag cgactcgtca aactgtctgc aatggacagg gcaaaagcag
     6061 ctaaatgtaa gtccggttat ccaaaccgta tgcctgttgg tagtggtaac cagttggcga
     6121 agtggaatgg ttggaattac ttccaccgaa aggaaccttg cgataacggg agcgaataat
     6181 gcctattcag caacttccgc ttatgaaagg tgtcggcaaa gacttccgaa acgccgacta
     6241 tatcgactat ctgccagtga atatgctggc tacacccaaa gaaatcctga acagcagcgg
     6301 atatcttcgc tcattcccgg gcattgccaa acgttctgac gtgaacggtg tatctcgcgg
     6361 cgtcgagtac aacatggcgc agaatgctgt ttatcgcgtg tgtggcggca agctgtacaa
     6421 aggagaaagt gaagtcggtg atgttgccgg aagtggtcgt gtatcaatgg cgcatggtcg
     6481 gacatcacag gcggtaggcg ttaatggtca actggtcgag tatcgctatg atggcacggt
     6541 caaaaccgtc tcaaactggc ctacagacag tggattcact cagtatgagt taggttcagt
     6601 tcgcgacatt acgcgcttac gtgggcgtta tgcgtggtca aaagacggaa ctgattcatg
     6661 gtttatcact gaccttgaag acgaatctca tcctgaccgt tacagcgcac aatatcgtgc
     6721 cgagtctcag cctgacggca tcatcggtat cgggacatgg cgagacttca tcgtctgctt
     6781 tggttcatcg actattgaat atttctccct gactggcgca accaccgttg gtgccgcttt
     6841 gtatgttgca caaccatcgc tgatggtgca gaaaggcatt gccggaactt actgcaaaac
     6901 gccattcgct gattcgtatg cgtttatcag caatccggca acgggtgcgc cgtctgtgta
     6961 tatcatcgac tccggtcagg tgtcaccaat tgccagcgcg agcattgaga aaatcctccg
     7021 ctcctacact gctgatgaac tggctgatgg tgtgatggaa tcgctgcgat ttgatgctca
     7081 tgagttgctg attatccacc ttccgcgtca cgtcctcgtg tacgacgcat cttcaatcgc
     7141 caatggccca caatggtgtg tgctgaaaac aggtctgtat gacgatgtgt accgcgctat
     7201 cgacttcgtt tacgaaggca atcagataac gtgcggcgat aagctggaat ctgttaccgg
     7261 gaaactgcaa ttcgatatca gcagccagta cgacaagcaa caggaacacc tgctgtttac
     7321 tccactcttc aaagcggata acgccagagt gttcgacctt gaggttgaat cgtcaactgg
     7381 cgttgcgcag tacgccgacc gcctttttct ctctgcaacc actgactgca tcaattacgg
     7441 gcgtgagcag atgattgagc agaatgaacc gttcgtttac gacaagcgtg ttttgtggaa
     7501 gcgagtaggg cgcatcagga aaaatgtcgg cttcaaattg cgcgttatca cgaagtcacc
     7561 tgtcactctg tctggcgctc agataaggat tgagtaatgg ctgattcgaa tctcaatgtg
     7621 ccggtaatca tccaagctac gcggctcgat acatcagtcc ttccacgcaa tatcttctcg
     7681 cagtcgtatc tgctttacgt tatcgcacag ggcactgatg ttggtaacgt ggctaacaaa
     7741 gccaacgagg ccggacaggg cgcttatgat gcacaggtca ggaacgatga gcaggatgtg
     7801 attctcgctg accatgagca gcgaatttct gctgcggaag caacgcttgt taatcatgag
     7861 gagcgaatca gacaggcaga atcaactctt caggaccatg aaacacgaat agctcagaat
     7921 gaaagcgata tttcgtcgct tgacacaaga gttcagtcgc tggagtcgca ggtttcagac
     7981 catgaaacgc gcatcgatgc tctggagtat gcaaccacac gcaagaagtc agaggttgtt
     8041 tactctggtg tatctgtaac catcccgaca gcgccgacca accttgttag cctgctgaaa
     8101 acgctcacac cgtcatccgg ctcgttggca ccattcttcg acaccgttaa caacaagatg
     8161 gttgtgttca acgagaacaa aaccctgctc ttcaagttgt cgattgtcgg cacgtggccc
     8221 agcggaaccg ccaacaggtc aatgcagcta accttttccg gctctgttcc tgacacactg
     8281 gtcagcagtc gtaatgcggc gacaacaacc gacaacatcc tgttagctac gttcttcagc
     8341 gtggataaag acggctttct tgccacaaat ggcagtacgt taaccattca gtcgaatggt
     8401 gcggcgttta ctgccacaac catcaagata atcgcggagc agtgatgatt acattcaaac
     8461 caacgcgaaa catcgacctg atagaagcag tcggaaatca ccctgacatt atcgccggga
     8521 gcaacaacgg tgatggatac gactacaaac ctgaatgccg ttactttgag gtgaacgtgc
     8581 acggtcagtt tggcggcatt gtttactatc aggagattca gccgctgaca ttagattgcc
     8641 acgccatgta cctgccagag attcgcggct tcagcaagga aattgggctg gcgttctggc
     8701 gatacattct gactaacacc accgttcagt gcgtaacatc gttcgctgca cgcaaattcc
     8761 gacacggtca gatgtactgc gcaatgattg gccttaagcg tgtaggaacc atcaagaaat
     8821 acttcaaagg cgtggatgac gtgacttttt acagcgccac acgcgaagaa ctaatcgact
     8881 tcctgaatca cgggagatag ccatgttata tgcatttaag ctgggcagaa aactgcgcgg
     8941 cgaggaacct tattgccctg aaaaaggcgg gaaaggtggc agttctgata aaagcgcaaa
     9001 gtatgcagca gaagcccaga agtatgctgc agacctgcaa aatcagcagt ggcagacgat
     9061 catgaaaaac cttgctccgt tcacgcctct tgcggagcag tatgttaacc agcttcagaa
     9121 cctttccagt ttagaaggtc aggggcaggc acttaatcag tattacaact ctaagcagta
     9181 taaagacctt gcagggcagg cgcgttacca gagtcttgct gctgcggagg cgacgggtgg
     9241 acttggttcg acagccacaa gcaatcaact ggctacgatc gcgccgacac tcggtcagtc
     9301 ttggttatca aatcagatga gcaattacaa caatctggca aacgttgggc ttggtgcgct
     9361 gcaaggtcag gcaaacgccg ggcagacgta cgccaacaac atgagcagca ttgcacagca
     9421 aagcgcagct cttgccgctg ctaatgccaa taaaccatca agtcttcaga ctgcaattag
     9481 cggtggcacg tctggtgcga ttgccggtgc aggtcttgcc agccttttgg gaacatcaac
     9541 gccttggggc gctggtattg gtgctggtat cggattgctt ggctcgttgt tttaaggggg
     9601 aatcatggct acttggcaag gaacaaacgg cggattgttg gctggtatcg gcggcgtcaa
     9661 ctcaaacgct ccgagcgtaa atgacatcgg caatacgctt cagcttatca ggcagaacaa
     9721 tgatattgag cgttcaggcg ctaacaatgt tgggctgact gctttgcaag gtctttcagg
     9781 tattgcgggt gtttttcagc aggaaaagca ggctcagcgg cagaaagaat ttcagcaggc
     9841 gtacgctaat gcttatgcgt ctggtgatcg cggtgctttg cgtcagttgg ctactcaata
     9901 tccagaccag attgaatccg ttcgtaaagg catgggattc attgatgaag agcagcgtaa
     9961 ttctatcggc accttagcgg ctggcgcacg ccttgcgtca tcgtctccag aagcaatgca
    10021 atcatggctg caaaacaacg ccggtgagtt agctcgtgtt ggcgttaatc ctcatgacgt
    10081 cgctcagatg taccaacaga acccgcggca gttcggcgaa tttgtcgatc acctggggat
    10141 gaacagtctc gggcccgaaa aatactttga cctacaggat aaaatgcagg gtcgccaggt
    10201 taccatgcgc ggtcaggatc tggattcgca aaccgccgct cggaatcagg caatcacaat
    10261 gcgcggacaa gatatccagg cgaatttagg tcagcagcgc attaatctgg acgcagaaac
    10321 aaaccgcatt aacaacgaaa ataagcgcct tgaccggatg ctatcagcag aaactaacga
    10381 cctgaagcgc caggaaatac agagccgcat agcagccaac aaccagcagt tgcagcagaa
    10441 gcagcaagcg ctaaatgatg gctacaaaga cggcatcaac accctcacaa ccagcatgtt
    10501 cactctgaac gatatcgtta gttctccttc acttaagagc attacaggct tacgtggagt
    10561 aatccccaac gttccaggct cacaggctgc agacactcag gcacgacttg atacctttaa
    10621 atcacaagca tacctgacag cggttcaggc catgcgaggc atgggcgcac tttctgatgc
    10681 cgagggcaaa aagctcgacc aggctgttgg ttcgctgcag aactcgcaga gcgaggagtc
    10741 ctttcgtcgc aacgctggcg tcatcctgaa cacgctcaac cagaagcgta atgaggcggt
    10801 tggtaagtac gttcagcaaa acggtatcaa gcgagtggaa gcgcctcagg cttctataga
    10861 ttacctgaag cagcaccccg agctgtcaat cgacttcatt aatcgctacg gatatcttcc
    10921 atctttgggg cagtaaatgg ctaattaccg tgatttgtta gagcaggctg gcgcacgtta
    10981 cggtgtgcca gaagggttga tgactgcact gggtgccaag gagtcttctt acaaccctgc
    11041 cgcagtaagc tccgccgggg ctgtaggatt gactcaggtc atgcctggga catggcgtga
    11101 tatgggttat accgatgagc aaatgcaaaa ccccgaatat caggctgacg ctggcgcgcg
    11161 ctatctggca aagatgtacc agcagtttgg taactggcgt gactctcttc aggcttatca
    11221 cgacggtccc ggcaacgtta tgaaggcaaa gcgtggtgaa tatacgccag gacctgaagg
    11281 ccgcggttac gttgatgatc gctttgctca atgggctggt gacccggtga cagactcaac
    11341 agtcgaacag cgcgccacct ctgcaaaggt acatcctcag caagacccta acaacccgtt
    11401 tgcacaactg gaagtacagt catccgaaca agtatcggca tcaggtgtgc agtcagaccc
    11461 aaataatcca tttgctcaga ttgagcagca ggcagccagt cagcagccac ctcaacccgt
    11521 aagttctgtc gcaccgaaac ctgttcagca acaaacagtt aatcaggcca ataatgcacc
    11581 aacacgggaa gaaccatcat tgatgcaaca agctggcgat tggctcactg gtggtcaaag
    11641 tgcagggcaa attgcagaac aggctggtcg tggtctggta aacataccat ttgacgtatt
    11701 gcagggtggc gcaagtctga ttaatgcaat cagtcagggg cttggggggc caaaagtatt
    11761 ggatgatgtt tatcgtccag tagacagacc gacagacccc tacgcgcaag ctggagagtc
    11821 aataggcggt tatcttgttc ctggagcagg tgtagctgga aacatggtca ttggttctct
    11881 cgctgacgcg gcgaatcaac ggggtgattt tgccgaaaat gccgctatta atgccggact
    11941 tagcattgct acgcatggcc tgataaatgg cgttacccgt ggtgttcgtg gtgcatcaaa
    12001 tataattagt ggcaataaaa catctgcaca gagagcgacc actgcgccaa cagaaacatc
    12061 accattctcc ggtgatgccg ctgcagcaac aaatcctgcg gttcatgccg cagaggcaag
    12121 agtagcacaa ggtgtaccaa tgacgcctgc gacgaagaac ccagaggaag tcgttcgcac
    12181 agtagcagca caaaaaaggc caaatctcgc ttcatcgctt gatgaactag atatcaatcc
    12241 tcaggctgaa gttctggagt ctgctgaaag gcttaatgtt gattcattac tcccttcaca
    12301 cttttccggg aacgagcaat acaaggcagt tgagcaagca atcaagtccc gtgcgggttc
    12361 tgctctacag gtgcaggaaa atgaagcaat caggcagcta gcacagggcg cgggtgagat
    12421 aattgatcgc gtttccggtg caaaagatgc tcttggtatg agcgacaagt ttattgatac
    12481 ggtcaatgga agaatgtctg cgctgatgaa acgaagcgac cagctttatc gcaatgttga
    12541 aaaggcgat
//
LOCUS       DAJTRC010000103        12438 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.103, whole genome shotgun sequence.
ACCESSION   DAJTRC010000103 DAJTRC010000000
VERSION     DAJTRC010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12438)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12438)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12438
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.103"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..1326)
                     /gene="uxaB"
                     /locus_tag="OO357_003240"
     CDS             complement(<1..1326)
                     /gene="uxaB"
                     /locus_tag="OO357_003240"
                     /EC_number="1.1.1.58"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HCR5888081.1"
                     /translation="MKTLNRRDFPGAQYPERIIQFGEGNFLRAFVDWQIDLLNEHTDL
                     NSGVVVVRPIETSFPPSLSTQDGLYTTIIRGLNEKGEAVSDARLIRSVNREISVYSEY
                     DEFLKLAHNPEMRFVFSNTTEAGISYHAGDKFDDAPAVSYPAKLTRLLFERFSHFNGA
                     LDKGWIIIPCELIDYNGDALRELVLRYAQEWALPEAFIQWLDQANSFCSTLVDRIVTG
                     YPRDEVAKLEEELGYHDGFLDTAEHFYLFVIQGPKSLATELRLDKYPLNVLIVDDIKP
                     YKERKVAILNGAHTALVPVAFQAGLDTVGEAMNDAEICAFVEKAIYEEIIPVLDLPRD
                     ELESFASAVTGRFRNPYIKHQLLSIALNGMTKFRTRILPQLLAGQKANGTLPARLTFA
                     LAALIAFYRGERNGETYPVQDDAHWLERYQQLWSQHRDRVIGTQELVAIV"
     gene            complement(1553..2971)
                     /locus_tag="OO357_003241"
     CDS             complement(1553..2971)
                     /locus_tag="OO357_003241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HCR5888082.1"
                     /translation="MHVHPISTFRLFQEGHLLRNSIAIFALTTLFYFIGAELRLVHEL
                     SLFWPLNGVMAGVFARYVWLNRLHYYAISYVAMLVYDAITTEWGLVSLVINFSNMMFI
                     VTVALLVVRDKRLGKNKYEPVSALRLFNYCLIAALLCAIVGAIGSVSIDSLDFWPLLA
                     DWFSEQFSTGVLIVPCMLTLAIPGVLPRFKAEQMMPAIAFIVSVIASVVIGGAGSLAF
                     PLPALIWCAVRYTPQVTCLLTFVTGAVEIVLVANSVIDISVGSPFSIPEMFSARLGIA
                     TMAICPIMVSFSVAAINLLMKQVALRADFDFLTQVYSRSGLYEALKSPSLKQTQHLTV
                     MLLDIDYFKSINDNYGHECGDKVLSVFARHIQKIVGDKGLVARMGGEEFAVAVPSVNP
                     VDGLLMAEKIRKGVELQPFTWQQKTLYLTVSIGVGSGRASYRTLTDDFNKLMVEADTC
                     LYRSKKDGRNRTSTMRYGEEVV"
     gene            complement(3125..3469)
                     /locus_tag="OO357_003242"
     CDS             complement(3125..3469)
                     /locus_tag="OO357_003242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4186 domain-containing protein"
                     /protein_id="HCR5888083.1"
                     /translation="MQSLDPLFARLSRSKFRSRFRLGMKERQYCLEKGAPVIEQHAAD
                     FVAKRLAPALPANDGKQTPMRGHPVFIAQHATATCCRGCLAKWHNIPQGVSLSEEQQR
                     YIVAVIYHWLVV"
     gene            complement(3469..4395)
                     /gene="glsB"
                     /locus_tag="OO357_003243"
     CDS             complement(3469..4395)
                     /gene="glsB"
                     /locus_tag="OO357_003243"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase B"
                     /protein_id="HCR5888084.1"
                     /translation="MAVAMDNAILENILRQVRPLIGQGKVADYIPALATVDGSRLGIA
                     ICTVDGQLFQAGDAQERFSIQSISKVLSLVVAMRHYSEEEIWQRVGKDPSGSPFNSLV
                     QLEMEQGIPRNPFINTGALVVCDMLQGRLSAPRQRMLEVVRGLSGVSDISYDTVVARS
                     EFEHSARNAAIAWLMKSFGNFHHDVTTVLQNYFHYCALKMSCVELARTFVFLANQGKA
                     IHIDEPVVTPMQARQINALMATSGMYQNAGEFAWRVGLPAKSGVGGGIVAIVPHEMAI
                     AVWSPELDDAGNSLAGIAVLEQLTKQLGRSVY"
     gene            complement(4459..5394)
                     /locus_tag="OO357_003244"
     CDS             complement(4459..5394)
                     /locus_tag="OO357_003244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001580861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HCR5888085.1"
                     /translation="MRGAVPIILAGNGYLLKHAPNVMGCAQLIAQVFKDAGIPQGVYG
                     WLNADNDGVSQMIKDSRIAAVTVTGSVRAGAALKKCVLELGGSDPFIVLNDADLELAV
                     KAAVAGRYQNTGQVCAAAKRFIIEEGIASAFTERFVSAAAALKMGDPRDEENALGPMA
                     RFDLRDELHHQVEKTLAQGARLLLGGEKMAGAGNYYPPTVLANVTPEMTAFREEMFGP
                     VAAITIAKDAEHALELANDSEFGLSATIFTTDETQARQMAARLECGGVFINGYCASDA
                     RVAFGGVKKSGFGRELSHFGLHEFCNIQTVWKDRI"
     gene            5474..6589
                     /gene="yneK"
                     /locus_tag="OO357_003245"
     CDS             5474..6589
                     /gene="yneK"
                     /locus_tag="OO357_003245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative protein YneK"
                     /protein_id="HCR5888086.1"
                     /translation="MVTPVSICNYISLPDDFPARNIAPQVKEVLKDFIDALSTIICDE
                     EWRTSLNTNSATKKIFNNLDNLSYIQRTSFRGNDTLYNEKVQFKLTYPVKNGRHKENI
                     EFQVVINLSPIYLDNFRHDGEINIFCAPNPKPVTMGRVFQTGVERVLFLFMNDFIEQF
                     PMINLGAPIKRAHTPHIEPLPPDHHTAADYLRQFDLLVLNFISRGNFVILPRLWNNSE
                     VHRWFVNKDPNLITAILDITDSELKEDLLQSLMDSLGSNKHVQPEVCICFLSLLAEQE
                     SPHFQDLFLFFANMLLHYHQFMNPNESDLNDVLMPASLSDDKIIKHMARRTLKLFVKN
                     ETPPKVTHEDLVKNRPRSPVRPPIPATAKTPDLPERH"
     gene            6739..7929
                     /gene="ydeA"
                     /locus_tag="OO357_003246"
     CDS             6739..7929
                     /gene="ydeA"
                     /locus_tag="OO357_003246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose MFS transporter"
                     /protein_id="HCR5888087.1"
                     /translation="MTTNTVSRKVAWLRVVTLAVAAFIFNTTEFVPVGLLSDIAQSFH
                     MQTAQVGIMLTIYAWVVALMSLPFMLMTSQVERRKLLICLFVVFIASHVLSFLSWSFT
                     VLVISRIGVAFAHAIFWSITASLAIRMAPAGKRAQALSLIATGTALAMVLGLPLGRIV
                     GQYFGWRMTFFAIGIGALVTLLCLIKLLPLLPSEHSGSLKSLPLLFRRPALMSIYLLT
                     VVVVTAHYTAYSYIEPFVQNIAGFSANFATALLLLLGGAGIIGSVIFGKLGNQYASAL
                     VSTAIALLLVCLALLLPAANSEIHLGVLSIFWGIAMMIIGLGMQVKVLALAPDATDVA
                     MALFSGIFNIGIGAGALVGNQVSLHWSMSMIGYVGAVPAFAALIWSIIIFRRWPVTLE
                     EQTQ"
     gene            complement(7954..8619)
                     /gene="marC"
                     /locus_tag="OO357_003247"
     CDS             complement(7954..8619)
                     /gene="marC"
                     /locus_tag="OO357_003247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter MarC"
                     /protein_id="HCR5888088.1"
                     /translation="MLDLFKAIGLGLVVLLPLANPLTTVALFLGLAGNMNSAERNRQS
                     LMASVYVFAIMMVAYYAGQLVMDTFGISIPGLRIAGGLIVAFIGFRMLFPQQKAIDSP
                     EAKSKSEELEDEPSANIAFVPLAMPSTAGPGTIAMIISSASTVRQSSTFADWVLMVAP
                     PLIFFLVAVILWGSLRSSGAIMRLVGKGGIEAISRLMGFLLVCMGVQFIINGILEIIK
                     TYH"
     gene            8827..9261
                     /gene="marR"
                     /locus_tag="OO357_003248"
     CDS             8827..9261
                     /gene="marR"
                     /locus_tag="OO357_003248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance transcriptional
                     regulator MarR"
                     /protein_id="HCR5888089.1"
                     /translation="MKSTSDLFNEIIPLGRLIHMVNQKKDRLLNEYLSPLDITAAQFK
                     VLCSIRCAACITPVELKKVLSVDLGALTRMLDRLVCKGWVERLPNPNDKRGVLVKLTT
                     SGAAICEQCHQLVGQDLHQELTKNLTADEVATLEHLLKKVLP"
     gene            9281..9664
                     /gene="marA"
                     /locus_tag="OO357_003249"
     CDS             9281..9664
                     /gene="marA"
                     /locus_tag="OO357_003249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MDR efflux pump AcrAB transcriptional activator
                     MarA"
                     /protein_id="HCR5888090.1"
                     /translation="MSRRNTDAITIHSILDWIEDNLESPLSLEKVSERSGYSKWHLQR
                     MFKKETGHSLGQYIRSRKMTEIAQKLKESNEPILYLAERYGFESQQTLTRTFKNYFDV
                     PPHKYRMTNMQGESRFLHPLNHYNS"
     gene            9696..9914
                     /gene="marB"
                     /locus_tag="OO357_003250"
     CDS             9696..9914
                     /gene="marB"
                     /locus_tag="OO357_003250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multiple antibiotic resistance protein MarB"
                     /protein_id="HCR5888091.1"
                     /translation="MKPLSSAIAAALILFSAQSVAEQTTQPVVTSCANVVVVPPSQEQ
                     PPFDLNHMGTGSDKSDALGVPYYNQHAM"
     gene            complement(9945..10844)
                     /gene="eamA"
                     /locus_tag="OO357_003251"
     CDS             complement(9945..10844)
                     /gene="eamA"
                     /locus_tag="OO357_003251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylserine/cysteine exporter"
                     /protein_id="HCR5888092.1"
                     /translation="MSRKDGVLALLVVVVWGLNFVVIKVGLHNMPPLMLAGLRFMLVA
                     FPAIFFVARPKVPLNLLLGYGLTISFAQFAFLFCAINFGMPAGLASLVLQAQAFFTIV
                     LGAFTFGERLHGKQLAGIALAIFGVLVLIEDSLNGQHVAMLGFMLTLAAAFSWACGNI
                     FNKKIMSHSTRPAVMSLVIWSALIPIIPFFVVSLILDGSASMIHSLVTIDMTTILSLM
                     YLAFVATIVGYGIWGTLLGRYETWRVAPLSLLVPVVGLASAALLLDERLTGLQFLGAV
                     LIMTGLYINVFGLRWRKAVKVGS"
     gene            11108..12226
                     /gene="ydeE"
                     /locus_tag="OO357_003252"
     CDS             11108..12226
                     /gene="ydeE"
                     /locus_tag="OO357_003252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001538769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux MFS transporter YdeE"
                     /protein_id="HCR5888093.1"
                     /translation="MATLPFMTIYLSRQYSLSVDLIGYAMTIALTIGVVFSLGFGILA
                     DKFDKKRYMLLAITAFASGFIAIPLVNNVTLVVLFFALINCAYSVFATVLKAWFADNL
                     SSTSKTKIFSINYTMLNIGWTIGPPLGTLLVMQSINLPFWLAAICSAFPMLFIQIWVK
                     RSEKIIATETGSVWSPKVLLQDKALLWFTCSGFLASFVSGAFASCISQYVMVIADGDF
                     AEKVVAVVLPVNAAMVVTLQYSVGRRLNPANIRALMTAGTLCFVIGLVGFIFSGNNLL
                     LWGMSAAVFTVGEIIYAPGEYMLIDHIAPPGMKASYFSAQSLGWLGAAINPLVSGVVL
                     TSLPPSSLFVILALVIIAAWVLMLKGIRARPWGQPALC"
     gene            12353..>12438
                     /gene="mgtS"
                     /locus_tag="OO357_003253"
     CDS             12353..>12438
                     /gene="mgtS"
                     /locus_tag="OO357_003253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein MgtS"
                     /protein_id="HCR5888094.1"
                     /translation="MLGNMNVFMAVLGIILFSGFLAAYFSHK"
BASE COUNT         3270 a         3190 c         2844 g         3134 t
ORIGIN      
        1 cacaatcgct accagttcct gggtaccgat aacacggtca cgatgctggc tccagagttg
       61 ctgataacgt tccagccagt gtgcatcgtc ctgtaccgga tatgtttctc cgttacgctc
      121 accacgatag aacgcaatca atgccgctaa tgcgaaagta aggcgcgccg gaagtgtgcc
      181 atttgccttc tgccctgcca gcagctgcgg caggatgcgg gtgcggaact tggtcatacc
      241 gttgagcgcg atagacagca gctgatgctt aatgtacggg ttacggaaac gcccggtgac
      301 tgcactggcg aaagattcca gttcatcacg aggcaaatcc agtaccggaa taatttcttc
      361 gtagatggct ttttcaacga atgcgcaaat ttcagcatcg ttcatcgctt cacctacggt
      421 atccagccct gcctggaacg ccaccggcac cagcgcggtg tgcgcaccgt tgaggattgc
      481 cactttgcgt tctttatacg gtttaatatc gtcaacaatc agcacgttga gcggatattt
      541 gtccaggcgc agctcagtcg ctaaggattt cggcccctga atcacaaaca ggtaaaagtg
      601 ttcagcggtg tcgaggaaac catcgtgata acccagttct tcttccagtt tagccacttc
      661 atcgcgcgga taaccggtaa cgatacggtc caccagcgta gaacagaagc tgttagcctg
      721 atccagccat tgaataaatg cttctggcag tgcccactct tgcgcatagc gcagcaccag
      781 ttcacgcaac gcgtcgccat tgtagtcaat caactcacac ggaatgatga tccaaccttt
      841 atccagcgca ccgttgaaat ggctaaagcg ttcgaacagc agacgggtca gttttgccgg
      901 atagcttacc gctggcgcgt catcgaattt atcgcccgca tggtagctga tacccgcttc
      961 ggtggtgtta gagaaaacaa agcgcatttc cgggttgtgc gccagtttca ggaattcatc
     1021 gtattcactg tagacgctga tttcacgatt aaccgagcga atcagacgcg cgtcgctgac
     1081 cgcttccccc ttctcattca ggccacggat aatggtggtg tacagaccat cctgcgtgct
     1141 cagtgacggc gggaatgaag tttcaatcgg acgaacaacg accacgccag aattcagatc
     1201 ggtgtgctca ttcaggagat cgatttgcca gtcaacaaag gcgcgcagga agttaccttc
     1261 accaaactga atgatacgtt ctggatactg tgcaccggga aaatcgcgac gatttagtgt
     1321 tttcacaatg ggttcccttc tgattagtca tacaacccgt ttgaattggt gcgacaggtt
     1381 agcaaacttt aatacgccga acccctgttt tgatcaactc ctgatgatta atgagcagtt
     1441 ttatgagaaa agtgtggcgc ggatcatggt ttaatcgagg aaatacgcct tttcctggat
     1501 cataaagtgg tagaacacat tgcattcaaa tcgcgcggaa tgaataaaga tgtcagacaa
     1561 cttcctcacc gtaacgcata gtgctggtac ggttgcgccc atctttcttc gagcgataca
     1621 gacacgtatc ggcttcgacc atcaatttat taaaatcatc ggtcagcgtt cggtacgatg
     1681 cgcgaccact accgacgcca atacttaccg tgagataaag cgttttttgt tgccaggtga
     1741 atggttgcag ttcaacgcct ttacggattt tttccgccat tagcagacca tcgacaggat
     1801 tcaccgacgg cactgcaaca gcaaattctt cgccgcccat gcgcgccacc agccccttat
     1861 caccgacaat cttctgaata tgccgggcaa acacgcttaa cactttatcg ccacattcat
     1921 gtccatagtt atcgttaatg cttttgaaat agtcgatatc aagcagcatg accgtcagat
     1981 gttgtgtctg tttcagcgat ggacttttca gcgcctcata aagaccggac cgtgagtaaa
     2041 cctgagtcag aaaatcaaag tcggctcgca gcgcaacttg cttcattagc aaattgatcg
     2101 ctgccacgct aaaagaaacc ataattgggc atatcgccat cgtggcaata ccgagacgtg
     2161 cggagaacat ttccggaatg gagaacggcg aaccgaccga gatatcaatc accgaatttg
     2221 ccaccagtac gatttccacc gcaccggtga caaaggtcaa cagacatgtt acctgcggcg
     2281 tatagcgcac tgcacaccag attaatgcag gcagcgggaa cgccagactc cccgctccgc
     2341 caatgactac cgaggcaata accgacacaa taaacgcgat ggcgggcatc atctgttctg
     2401 ctttaaagcg cggcagcact ccaggaatag ccaacgtcag catacaaggc acgatcaaca
     2461 cgcccgttga gaattgctca ctgaaccagt ccgcaagcaa aggccagaaa tccagactat
     2521 caatactgac cgaaccaatc gccccgacaa tagcgcataa taatgcggca atcagacagt
     2581 aattaaatag ccgtaaagca cttactggct catacttatt tttccccaga cgcttatccc
     2641 gcacgaccag taaggcgacg gtaacaataa acatcatatt ggagaaattg ataaccagtg
     2701 aaaccagtcc ccattcggtg gttatggcat cataaaccag catcgccaca taactgatcg
     2761 catagtaatg cagtcgatta agccagacat agcgggcaaa cacccccgcc attacgccat
     2821 tcagcggcca gaaaagagaa agttcgtgaa ccagccgtaa ctctgcacca ataaaataga
     2881 acagcgtggt tagcgcaaaa atagcgatgc tattacgtag cagatgacct tcctggaaca
     2941 aacggaaagt tgaaatgggg tgtacatgca ttaattatct gtccatacaa ttgcgggtgg
     3001 caattatctc aacgtgataa gtaaattctc ataaaggata gcatgttcgc taacagcggc
     3061 ggcaaataaa ggtaccctgg gaatgctatt ttatcgtttt ccgcaattct catggctgat
     3121 tcatctatac aaccagccag tgatagatta ccgccacgat ataacgttgt tgttcttcgc
     3181 ttaacgaaac accctgcgga atgttatgcc acttagccag acaaccgcga cagcaggtcg
     3241 cggtggcgtg ctgagcgata aacaccggat gcccgcgcat gggggtttgc ttaccgtcat
     3301 tagccggtaa tgccggagca agacgtttag cgacaaaatc cgccgcatgt tgttcgatga
     3361 ctggcgcgcc tttctccagg caatactgac gctctttcat gcccagacga aagcgagagc
     3421 gaaattttga acgggataaa cgcgcgaaga gtggatcgag agactgcatt aataaaccga
     3481 acgccctaac tgtttcgtca attgttcaag gacggcgata cccgcaagcg agttacctgc
     3541 atcatccagt tccggactcc agactgcgat ggccatttca tgcggaacaa tcgccacaat
     3601 accgccacca acgccagatt tcgccggtag tcccacccgc caggcaaact cccccgcgtt
     3661 ctggtacata ccactggtcg ccatcagcgc gttaatttgc cgcgcctgca ttggcgtcac
     3721 cacaggttca tcaatatgaa tagctttccc ctgattagcc agaaagacaa acgtccgggc
     3781 cagctctaca cagctcattt tcagagcgca gtaatgaaag tagttttgca gaacggttgt
     3841 cacgtcatga tggaaattgc caaacgactt catcagccag gcgatagccg catttcgcgc
     3901 ggaatgttca aattcggaac gcgctaccac cgtatcgtat gaaatatcag acacaccgct
     3961 taagccgcgc acgacttcca gcatacgttg ccgtggtgcg cttagtcgcc cttgcaacat
     4021 atcgcagacc accagcgcac cggtattaat gaacggatta cgcggaatac cctgttccat
     4081 ttccagttgc actaaagaat tgaacggtga tccagacgga tctttgccga cgcgttgcca
     4141 gatttcctct tcggagtaat gacgcatggc gacaacgaga ctcagcactt tggaaataga
     4201 ctgaatggaa aaacgttctt gcgcgtctcc ggcctgaaaa agctgtccgt caacggtaca
     4261 gatagcaatc cccaatcggg aaccgtctac tgtagccagc gccggaatat aatccgcgac
     4321 tttaccctga ccaatgagcg gccgcacttg ccgcaagatg ttttctaaaa ttgcattatc
     4381 catggcgact gccactttct actcctggac cgcaggtctg aaaagacctg cgagtatatc
     4441 agagctgaat atgtcgcgtc agatccggtc tttccacact gtctggatat tacagaattc
     4501 gtgtaagccg aaatgggaaa gctcacgacc aaagccactc tttttcacgc caccaaaggc
     4561 cactcgcgcg tcgctggcac aataaccatt gataaacacc ccaccgcatt ccagacgtgc
     4621 cgccatctgt ctggcctgtg tttcgtcggt ggtaaaaatg gtcgctgaaa ggccgaactc
     4681 actatcatta gccagttcca gcgcatgttc tgcatctttc gcaatggtga ttgcagcaac
     4741 ggggccaaac atttcttccc gaaacgcggt catttctggg gtaacattcg ccagaaccgt
     4801 tggcggatag tagttacctg ctccagccat cttttccccg cccagtaaca aacgcgcacc
     4861 ctgcgccagg gttttctcca cctgatgatg cagctcatca cgtaaatcaa aacgagccat
     4921 tggtccgaga gcgttctctt catcacgggg atcgcccatt ttcaaggctg ccgcagctga
     4981 cacaaaacgt tcggtaaatg ccgaagcaat tccctcttcg ataataaagc gttttgccgc
     5041 tgcacaaacc tgtccggtat tctgataacg tccggctacc gccgctttaa ccgccagttc
     5101 cagatcggca tcgttaagca caataaatgg atccgaaccg cccagttcca gtacgcattt
     5161 tttcagtgcc gctccagcac gaacacttcc ggtcaccgtg acagcagcaa tgcgcgagtc
     5221 tttaatcatc tgactgacac cgtcgttgtc ggcattcagc cagccatata cgccttgcgg
     5281 gattcccgca tctttaaaca cttgggcaat gagctgagca cagcccatca cattcggcgc
     5341 atgtttaagt aagtagccgt tacctgcaag aatgatggga acagcgccac gcatcacctg
     5401 cctttgatag ttcttgcagc tcaaatgccc tatatagtga tgttccgatg acttatgact
     5461 atatggggca aatatggtta cgccagtaag catctgcaat tacatatctc ttcctgatga
     5521 ttttcccgct cgtaatattg cacctcaagt aaaagaagtt ttaaaggatt ttattgatgc
     5581 actcagtacc ataatatgtg atgaagagtg gcgcacgtct ttaaacacca actcagccac
     5641 aaaaaagata tttaacaacc ttgataactt atcatatatt cagaggactt cttttcgggg
     5701 taacgacaca ctatacaatg aaaaggttca gtttaaactc acttatcctg tcaagaatgg
     5761 aagacacaaa gaaaatattg aatttcaggt agtaataaat ttaagcccta tttatttaga
     5821 taattttcgc catgatggag aaattaatat tttttgcgct cccaacccaa agcctgtcac
     5881 tatggggcgc gtatttcaga ccggcgtcga gcgtgtgctc tttctgttta tgaatgattt
     5941 tattgaacaa tttccaatga tcaaccttgg cgcccccatc aaaagagcgc atacaccaca
     6001 tattgaaccc ctgcctccgg atcaccatac cgctgcagat tacttacgcc agtttgattt
     6061 gcttgtcctg aattttatct ctcgcggtaa ttttgtcata ctcccccgat tatggaataa
     6121 ctctgaggtt cacagatggt tcgtcaataa agatcctaac ttgatcaccg ccattctcga
     6181 cataacggac agcgaattaa aagaggattt attgcaaagc ctgatggatt cattaggttc
     6241 taacaaacat gtacaacccg aagtctgcat ctgcttctta tcccttttag cagaacaaga
     6301 atctcctcat tttcaggact tgtttttatt tttcgccaat atgttattgc actatcacca
     6361 atttatgaat cccaatgaaa gtgatctgaa tgacgtgttg atgccagcat cattaagtga
     6421 tgataaaatt atcaaacata tggcgcgcag gaccctcaaa ctgtttgtaa aaaatgaaac
     6481 gccgccaaaa gttactcacg aagacctggt gaaaaacagg cctcgctccc ctgtcagacc
     6541 acctataccc gcaaccgcca aaacgccaga cctccctgaa cgtcattaaa ccgtgatgtt
     6601 accgactctc tgactcggaa cagaatcagc gtcagagaaa cggaaaacgc gatccagatc
     6661 acaaatgcat tgtattcaca tcattaaccg ttttaagatc atttcatcac tttttcgcaa
     6721 ctcacccgat aatctgttat gacaacaaac actgtttccc gcaaagtggc gtggctacgg
     6781 gtcgttacgc tggcagtcgc cgccttcatc ttcaacacca ccgaatttgt ccctgttggc
     6841 ctgctctctg acattgcgca aagttttcac atgcaaaccg ctcaggtcgg catcatgttg
     6901 accatttacg catgggtagt agcgctaatg tcattgcctt ttatgttaat gaccagtcag
     6961 gttgaacggc gcaaattact gatctgcctg tttgtggtgt ttattgccag ccacgtactg
     7021 tcgtttttat cgtggagctt taccgttctg gtgatcagtc gcattggtgt ggcttttgca
     7081 catgcgattt tctggtcgat tacggcgtct ctggcgatcc gtatggctcc ggccgggaag
     7141 cgggcacagg ccttgagttt aattgccacc ggtacagcac tggcgatggt cttaggttta
     7201 ccgctcgggc gcattgtggg gcagtatttc ggctggcgaa tgaccttctt cgcgattggt
     7261 attggggcgc ttgttaccct tttgtgcctg attaagttac ttcccttact gcctagtgag
     7321 cattccggtt cattgaaaag cctcccgcta ttgttccgcc gcccggcatt gatgagcatt
     7381 tatttgttaa ctgtggtggt tgtcaccgcc cattacacgg catacagcta tatcgagcct
     7441 tttgtgcaaa acattgcggg attcagcgcc aactttgcca cggcattact gttattactc
     7501 ggtggtgcgg gcattattgg cagcgtgatt ttcggtaaac tgggtaatca atatgcgtct
     7561 gcgttggtaa gtacggcgat tgcgctgttg ctggtgtgtc tggcactgct gctacctgcg
     7621 gcgaacagtg aaatacacct cggggtgctg agtattttct gggggatcgc gatgatgatc
     7681 atcgggcttg gtatgcaggt taaagtgctg gcgctggcac cagatgctac cgacgtcgcg
     7741 atggcgctat tctccggcat atttaatatt ggaatcgggg cgggtgcgtt ggtaggtaat
     7801 caggtgagtt tgcactggtc aatgtcgatg attggttatg tgggcgcggt gcctgctttt
     7861 gctgcgttaa tatggtcaat cattatattt cgccgctggc cagtgacact cgaagaacag
     7921 acgcaatagt tgaaaggccc attcgggcct tttttaatgg tacgttttaa tgatttccag
     7981 gatgccgtta ataataaact gcacacccat acataccagc aggaacccca tcaggcggga
     8041 gatcgcttca atgccaccct tgcccaccag ccgcataatt gcgccggagc tgcgtaggct
     8101 tccccacaaa ataaccgcca ccaggaaaaa gatcagcggc ggcgcaacca tcagtaccca
     8161 atcagcgaag gttgaactct gacgcactgt ggacgccgag ctaataatca tcgctatggt
     8221 tcccggaccg gcggtacttg gcattgccag tggtacaaag gcaatattgg cactgggttc
     8281 atcttccagc tcttccgact tgcttttcgc ctccggtgaa tcaatcgctt tctgttgcgg
     8341 aaaaagcatc cgaaaaccga taaacgcgac gattaagccg cctgcaattc gcagaccggg
     8401 aatcgaaatg ccaaatgtat ccatcaccag ttgcccagcg taatacgcca ccatcatgat
     8461 ggcaaatacg tacaccgagg ccatcaacga ctgacgatta cgttcggcac tgttcatgtt
     8521 gcctgccagg ccaagaaata gcgcgacagt tgttaatggg ttagctaacg gcagcaacac
     8581 caccagcccc aggccaattg ctttaaacaa atctaacatt ggtggttgtt atcctgtgta
     8641 tctgggttat cagcgaaaag tataaggggt aaacaaggat aaagtgtcac tctttagcta
     8701 accttgcatc gcattgacca aaacttgaac cgatttagca aaacgtggca tcggtcaatt
     8761 catttgactt atacttgcct gggcaatatt accccctaca actaattact tgccaaggca
     8821 actaatgtga aaagtaccag cgatctgttc aatgaaatta ttccattggg tcgcttaatc
     8881 catatggtta atcagaagaa agatcgtctg cttaacgagt atctgtctcc gctggatatt
     8941 accgcggcac agtttaaggt gctctgctct atccgctgcg cggcgtgtat tactccggtt
     9001 gaactgaaaa aagtgttgtc ggtcgacctg ggagcactga cccgtatgct ggatcgcctg
     9061 gtctgtaaag gctgggttga aaggttgccg aacccgaatg ataagcgcgg cgtactggta
     9121 aaacttacca ccagcggcgc ggcaatatgc gaacaatgcc atcaattagt tggccaggac
     9181 ctgcatcaag aattaacaaa aaacctgacg gcggacgaag ttgcaacact tgagcatttg
     9241 cttaagaaag tcctgccgta aacaaaaaag aggtatgacg atgtccagac gcaatactga
     9301 cgctattacc attcatagca ttttggactg gatcgaggac aacctggaat cgccactgtc
     9361 actggagaaa gtgtcagagc gttcgggtta ctccaaatgg cacctgcaac ggatgtttaa
     9421 aaaagaaacc ggtcattcat taggccaata catccgcagc cgtaagatga cggaaatcgc
     9481 gcaaaagctg aaggaaagta acgagccgat actctatctg gcagaacgat atggcttcga
     9541 gtcgcaacaa actctgaccc gaaccttcaa aaattacttt gatgttccgc cgcataaata
     9601 ccggatgacc aatatgcagg gcgaatcgcg ctttttacat ccattaaatc attacaacag
     9661 ctagttgata acgtgacaac gtcactgagg caatcatgaa accactttca tccgcaatag
     9721 cagctgcgct tattctcttt tccgcgcaga gcgttgcgga acaaaccacg cagccagttg
     9781 ttacttcttg tgccaatgtc gtggttgttc ccccatcgca ggaacaacca ccgtttgatt
     9841 taaatcacat gggtactggc agtgataagt cggatgcgct cggcgtgccc tattataatc
     9901 aacacgctat gtagtttgtt ctggccccga catctcgggg cttattaact tcccaccttt
     9961 accgctttac gccaccgcaa gccaaataca ttgatataca gcccggtcat aatgagcacc
    10021 gcacctaaaa attgcagacc cgttaagcgt tcatccaaca atagtgccgc acttgccagt
    10081 ccgactacgg gcaccagtaa cgataacggt gcaacccgcc aggtttcata gcgtcccagt
    10141 aacgtccccc agatcccata accaacaatt gtcgccacaa acgccagata catcagcgac
    10201 aagatggtgg tcatatcaat agtaaccaga ctgtgaatca tggatgcgga accatcgaga
    10261 atcagcgaga caacaaagaa gggaatgatt gggattaaag cgctccagat taccagcgac
    10321 atcaccgccg gacgcgttga gtgcgacatg atctttttat tgaagatgtt gccacacgcc
    10381 caactaaatg ctgcagccag ggtcaacata aagccgagca tcgccacatg ctgaccgttc
    10441 agactatctt cgattaacac cagtacgcca aaaatcgcta aggcgatccc cgccaattgt
    10501 ttgccatgca gtcgctcccc gaaagtaaac gcgccaagca cgatagtaaa aaacgcctgt
    10561 gcctgtaaca ccagcgaagc cagtccagca ggcataccaa agttaatggc gcagaacaga
    10621 aaggcaaact gcgcaaaact gatggttaat ccatacccca agagcaaatt cagtggtact
    10681 ttcggtcgtg caacaaaaaa gatggccgga aaagcgacca gcataaagcg caaaccggcc
    10741 agcatcagcg gtggcatgtt atgaagcccc actttgatga ccacaaaatt tagcccccat
    10801 acgaccacta ccagtagcgc caacacccca tcttttcgcg acattctacc gcctctgaat
    10861 ttcatctttt gtaagcaatc aacttagctg aatttacttt tctttaacag ttgattcgtt
    10921 agtcgccggt tacgacggca ttaatgcgca aataagtcgc tatacttcgg atttttgcca
    10981 tgcgtccgat ggttaataac aacgacgagg caagaagggc gctggtagag cgtcgtaggg
    11041 ataagttcat ttgtctgtcc ggcagtgcgt ttcgttatgt tttattttag agatgtaaag
    11101 aaatagcatg gctacgctgc catttatgac catttacttg agtcgccagt acagcctgag
    11161 tgtcgatcta atcggttatg cgatgacaat tgcgctcact attggcgtcg tttttagcct
    11221 cggttttggt atcctggcgg ataagttcga caagaaacgc tatatgttac tggcaattac
    11281 cgccttcgcc agcggtttta ttgccattcc tttagtgaat aacgtgacgc tggttgtgct
    11341 cttttttgcc ctcattaact gcgcctattc tgtttttgct accgtgctga aagcctggtt
    11401 tgccgacaat ctttcgtcca ccagcaaaac gaaaatcttc tcaatcaact acaccatgct
    11461 aaacattggc tggaccatcg gtccgccgct cggcacgctg ttggtgatgc agagcatcaa
    11521 tctgcccttt tggctggcag ctatctgttc cgcgtttccc atgcttttca ttcaaatatg
    11581 ggtaaagcgc agcgagaaaa tcatcgccac ggaaacaggc agtgtctggt cgccgaaagt
    11641 tttattacaa gataaagcac tgttgtggtt tacctgctct ggttttctgg cttcttttgt
    11701 aagcggcgca tttgcttcat gcatttcaca atatgtgatg gtgattgctg atggggattt
    11761 tgccgaaaag gtggtcgcgg ttgttcttcc ggtgaatgct gccatggtgg ttacgttgca
    11821 atattccgtg ggccgccgac ttaacccagc taacatccgc gcgctgatga cagcaggcac
    11881 cctctgtttc gtcatcggac tggtcggttt tattttttcc ggcaacaacc tgctattgtg
    11941 gggtatgtca gctgcggtat ttactgtcgg tgaaatcatt tatgcgccgg gcgagtatat
    12001 gttgattgac catattgcgc cgccaggaat gaaagccagc tatttttccg cccagtcttt
    12061 aggctggctt ggtgccgcga ttaacccatt agtgagtggc gtagtgctaa ccagcctgcc
    12121 gccttcctcg ctgtttgtca tcttagcgtt ggtgatcatt gctgcgtggg tgctgatgtt
    12181 aaaagggatt cgtgcaagac cgtgggggca gcccgcgctt tgttgattta agtcgaacac
    12241 aataaaaatt taattcagcc ttcgtttagg ttatctctgc taatatcttt ctcattgaga
    12301 tgaaaattaa ggtaagcgag gaaacacacc acaccataaa cggaggcaaa taatgctggg
    12361 taatatgaat gtttttatgg ccgtactggg aataatttta ttttctggtt ttctggccgc
    12421 gtatttcagc cacaaatg
//
LOCUS       DAJTRC010000104        12262 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.104, whole genome shotgun sequence.
ACCESSION   DAJTRC010000104 DAJTRC010000000
VERSION     DAJTRC010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12262)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12262)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12262
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.104"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..98
                     /locus_tag="OO357_003254"
     CDS             <1..98
                     /locus_tag="OO357_003254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5888095.1"
                     /translation="FEDARDRIIAAEMPKQLGYALKQQLRLHLSK"
     gene            95..496
                     /locus_tag="OO357_003255"
     CDS             95..496
                     /locus_tag="OO357_003255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5888096.1"
                     /translation="MNRHTQIRQSVLARLREQCGDSATFFDGLPAFIDAQELPAVAVW
                     LSDAQYTGKMTDEDDWQAVLHIAVFVRAQAPDSELDTWMENTIFPAMKDIPVLSGLID
                     TMIPLGFNYQRDNEMATWAMAEITYQITYTN"
     gene            507..1247
                     /locus_tag="OO357_003256"
     CDS             507..1247
                     /locus_tag="OO357_003256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HCR5888097.1"
                     /translation="MTTPNPLAKTKGAGTTFWMYTGNGDAFANPLSDTDWLRLAMVKD
                     LQPGEMTADAEDDTYLDDEDADWKTTTQGQKSVGDTSATLAWRPGDSGQKKLVQLFDS
                     GEVCAFRIKYPNGTVDVFRGWLSSLGKTIASKDVMTRTVKISGVGRPYLAEEGTETVG
                     VTGLTVAPASVSVKAGATTTLTFTVKPDGASDKAISVHSSDPQTASVTLSGLVATVKG
                     VKQGSVSIVGMTSDGEFVAVAAVTVSAP"
     gene            1306..1692
                     /locus_tag="OO357_003257"
     CDS             1306..1692
                     /locus_tag="OO357_003257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein G"
                     /protein_id="HCR5888098.1"
                     /translation="MFLKQDTFNYEKQSVVLSELSGLQRIEYLTFVQQRTAKFDAQEG
                     ELPEAERQIAFLRMGMDINAWLVSRSLWNAEQSQDVETLCASIMTTWSYDALGAGAER
                     VLSLSGMGTIENAGDDDHEALTPEKS"
     gene            1701..2030
                     /locus_tag="OO357_003258"
     CDS             1701..2030
                     /locus_tag="OO357_003258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail assembly protein T"
                     /protein_id="HCR5888099.1"
                     /translation="MQFVMRLAREFRRADWRRMLSDMSATELGEWGDYFRMQSFSDVW
                     MDAQFASLKALIVRMVSGSSDAAVADFSLLPEENGIPERTDEELMHLGEGISGGVRYG
                     PDSQPGH"
     gene            2002..5043
                     /locus_tag="OO357_003259"
     CDS             2002..5043
                     /locus_tag="OO357_003259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5888100.1"
                     /translation="MDQIANLVIDLGIDAAEFKNEIPRIKNLLNGAASDAERSSARMQ
                     RFMERQTQAARQTMQAASSAATAASAHAQTVEKNARAHERMAREVEQTRLRVDALNQK
                     MREEQAQARALAEAQDKAAAAFYRQIDSVKQAGAGLQELQRIQQQIRQARNSGGVGQQ
                     DYLALISEITAKTRALTQAEEQATRQKAAFIRQLKEQATRQNLSSSELLRARAAQLGV
                     SSAAEVYIRKMERAGKATHSLGLKSAAARRELGVLISQMARGNFGALRGSGITLANRA
                     GWTGALMSPKGMMTGGVIGGLVAAVLGLGKAWHDGRKEGEKFNRQLALTGHYAGVTVG
                     QLWKLSRAISGNGITQHAAAGALAQVVGSGAFHGNDIGMVAKAAAQMERSVGQSVSDT
                     INQFKRLKDDPVNAAKALDNALHFLNATQLEQIRVLGEQGRSSDAARIAMSALAEETG
                     KRTSDIDNNLNALGSTLQTLSDWWKQFWDAAMNIGREDSLDAQIDALQEKIQRAKKYP
                     WTNASTQVEYDQQRLNDLQEKKRRKDLQDAKAQAERNYQEQQKRRNAENAALNRMNET
                     EAARHQREIARINAMQYADQAVRDAAIQRENERYEKAIKKNTRATRNDEATRLLLQYS
                     QQQAQVEGQIAAARQSAGIATERMTEAHKQLLALQQRISDLDGKKLTADEKSVLARKN
                     ELIQALTLLDVKQQELQKQTALNDLRKKTVQLTSQLADKERALREQHNLDIATAGMGD
                     KQRQRYQAQLRIRQEYRQQLQQLENDSRQKGTYGTEDYRRAEEVLKGSLKRQLNENKR
                     YWQELEVAQGDWKNGAMRAFQNFTADADNAAGTAEQMFTVAFSSAGNALATFCTTGKL
                     NFKSFTSSLLSDMARIMAQMAMMQAVKGVGSLFGFTTNADGGVYQSADLSRYSGTVVN
                     RPTFFAFAKGAGVMGEAGPEAILPLRRGADGKLGVVADTGGSGMVMFAPQYNIEINND
                     GTNGQIGPAALKVVYDLGKKAAADFMQQQARDGGRLSGAYR"
     gene            5043..5372
                     /locus_tag="OO357_003260"
     CDS             5043..5372
                     /locus_tag="OO357_003260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5888101.1"
                     /translation="METFHWKVRPDMNVVSEPKVVTVKLGDGYEQRRAAGLNNQLSTY
                     SVTIRVRKGEHPSLKAFLERHGGVRAFQWTPPYDWKLIRVVCRKWSASVGALWVTVTA
                     DFEQVVN"
     gene            5372..6070
                     /locus_tag="OO357_003261"
     CDS             5372..6070
                     /locus_tag="OO357_003261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage minor tail protein L"
                     /protein_id="HCR5888102.1"
                     /translation="MQDIRQETLNECTRAEQSARVELWEIDLTEVGGERYFFCNEQNE
                     KGEPVTWQGRQYQAYPIQGSGFELNGRGCAARPTLTVSNLHGMVTGMAEDLQSLVGGT
                     VVRRKVYARFLDAVNFVNGNSDADPEQEVISRWRIEQCSELSAVSASFVLATPTETDG
                     AVFPGRIMLANTCMWTYRSDECGYTGRAVADEFDKPTTDIRKDKCSKCMRGCELRNNT
                     GNFGGFLSINKLSQ"
     gene            6075..6818
                     /locus_tag="OO357_003262"
     CDS             6075..6818
                     /locus_tag="OO357_003262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5888103.1"
                     /translation="MTQTESAILAHARRCAPAESCGFVVRTPEGDRYLPSENISGEPE
                     ERFRMAPEDWLRAQMQGEIVALVHSHPGGLPWLSEADRRLQVQSDLPWWLVCRGTIHK
                     FRCVPHLTGRRFEHGVTDCYTLFRDAYHLAGIEMPDFHRGDDWWRHGQNLYLDNLEAT
                     GLYQVPLSSAQPGDVLLCCFGSSVPNHAAIYCGDGELLHHIPEQLSKRERYTDKWQRR
                     THSLWRHREWHASAFTGICNDLAAASTFV"
     gene            6755..7357
                     /locus_tag="OO357_003263"
     CDS             6755..7357
                     /locus_tag="OO357_003263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000090847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly protein"
                     /protein_id="HCR5888104.1"
                     /translation="MARICLYGDLQRFGRRIDLRVKTGAEAIRALATQLPAFRQKLSD
                     GWYQVRIAGRDAGETELSARLNEPLANGAVIHIVPRLVGAKSGGVFQAVLGAAVMAVA
                     IWMPGVGIMASNLLFSLGASMTLGGVAQMLAPKARTPRTQTTDNGKQNTCFSSLDNMV
                     AQGNVLPVLYGEMRVGSRVVSQEISTADEGDGGQVVVIGR"
     gene            7418..10897
                     /locus_tag="OO357_003264"
     CDS             7418..10897
                     /locus_tag="OO357_003264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="host specificity protein J"
                     /protein_id="HCR5888105.1"
                     /translation="MGKGSSKGHTPREAKDNLKSTQLLSVIDAISEGPVDGPVDGLKS
                     VLLNGTPVLDSEGKTNFSGVTVVFRAGEQEQTPPEGFESSGSETVLGTEVKYDTPITR
                     TITSANIDRLRLTFGVQALVETTSKGDRNPSEVRLLVQIQRNGGWVTEKDITIKGKTT
                     SQYLASVVVGNLPPRPFNIRMRRMTPDSTTDQLQNKTLWSSYTEIIDVKQCYPNTALV
                     GVQVDSEQFGNQQVSRNYHLRGRILQVPSNYNPQTRQYSGIWDGTLKPAYSNNMAWCL
                     WDMLTHPRYGMGKRLGAADVDKWALYVIGQYCDQSVPDGFGGTEPRITCNAYLTTQRK
                     AWDVLSDFCSAMRCMPVWNGQTLTFVQDRPSDKVWTYNRSNVVMPDDGAPFRYSFSAL
                     KDRHNVVEVNWIDPDNGHETATELVEDTQAIVRYGRNVTKMDAFGCTSRGQAHRAGLW
                     LIKTELLETQTVDFSVGAEGLRHVPGDVIEICDDDYAGISTGGRVLAVNSQTRTLTLD
                     REITLPSSGTTLISLVDGNGNPVSVEVQSVTDGVKVKVSRVPDGVAEYSVWGLKLPTL
                     RQRLFRCVSIRENDDGTYAITAVQHVPEKEAIVDNGAHFDGDRRGTVNGVTPPAVQHL
                     TAEVTADSGEYQVLARWDTPKVVKGVSFLLRLTVAADDGSERLVSTARTTETTYRFRQ
                     LALGNYSLTVRAVNARGQQGDPASVSFRIAAPAAPVTIELIPGYFQITVVPKLAVYDP
                     TVQFEFWFSEKRIADIRQVETSARYLGTALYWIAASINIRPGHDYYFYVRSVNTVGKS
                     AFVEAVGRASDDAEGYLSFYKGLINKTHLGKELWTQIDNGQLAPDLTEIRTSITNVSN
                     EITQTVNKKLENQSAAIQQIQKVQVDTNNNLNSMWAVKLQQMKDGRLYIAGIGAGIEN
                     TPAGMQSQVLLAADRIAMINPANGNTKPMFVGQGDQIFMNDVFLKRLTAPTITSGGNP
                     PAFSLTPGGRLTAKNADISGNVNANSGTLNNVTINKNCRVLGKLSANQIEGDLVKTVG
                     KPFPRDSRAPERWPSGTITVRVYDDQPFDRQIVIPAVAFRGAKHERKNNNIYSSCRLI
                     VKKNGAEIYNRTTLDNTLIYTGVIDMPAGHGHMTLEFSVSAWLVNGWYPTASISDLLV
                     VVMKKSTAGISIS"
     gene            10965..11564
                     /locus_tag="OO357_003265"
     CDS             10965..11564
                     /locus_tag="OO357_003265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ail/Lom family outer membrane beta-barrel
                     protein"
                     /protein_id="HCR5888106.1"
                     /translation="MRKLYAAILSAAICLAVSGAPAWASEHQSTLSAGYLHASTNAPG
                     SDDLNGINVKYRYEFTDTLGLVTSFSYANAEDEQKTHYSDTRWHEDSVRNRWFSVMAG
                     LSVRVNEWFSAYAMAGVAYSRVSTFSGDYLRVTDNKGKTHDVLTGSDDNRHSNTSLAW
                     GAGVQFNPTESVAIDLAYEGSGSGDWRTDGFIVGVGYKF"
     gene            11628..>12262
                     /locus_tag="OO357_003266"
     CDS             11628..>12262
                     /locus_tag="OO357_003266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004981847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5888107.1"
                     /translation="MAVKISGVLKDGAGKPVVNCAIELRARRTSPTVVAHVVATCVTD
                     NNGAYVIEAEPGYYEVALHCNGWQPTRVGDIDVAPTDAPGTLNAFLNAPKDGDLRPEV
                     MKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAATARDDAQRFAEKARQDATVTAEDRK
                     ATAEDVTSTGANAAAAGQSAQDAAGYARAAEQAKNDIDAALTGTLKMANHLS"
BASE COUNT         2887 a         2913 c         3925 g         2537 t
ORIGIN      
        1 catttgaaga tgcccgcgac cgcatcattg ctgcggaaat gccgaaacag ctggggtatg
       61 ccctgaaaca acaactgagg ttacatctga gtaaatgaac cgacacacac aaatccgtca
      121 gagcgtgctg gcacgccttc gggaacagtg tggagacagc gccacgtttt ttgacgggct
      181 tccggcattt attgatgcgc aggaactgcc cgccgtggcg gtgtggctga gtgatgcgca
      241 gtacaccgga aaaatgacgg atgaggacga ctggcaggct gttctgcata ttgccgtttt
      301 cgtccgtgca caggcgccgg attctgagct ggatacgtgg atggagaaca ccatatttcc
      361 ggccatgaag gatattccgg tgctttccgg actcatcgac accatgatcc cactcggttt
      421 taactatcaa cgtgataatg agatggccac ctgggcgatg gcggaaatca cgtaccagat
      481 cacgtacacg aattaaggag gtggtaatga ccacaccaaa tccactggca aagacgaaag
      541 gtgcggggac gacgttctgg atgtataccg gcaacggcga tgcgtttgcg aaccctttgt
      601 cggacactga ctggctgcgt cttgcgatgg tgaaggatct gcaacctggc gaaatgaccg
      661 ctgatgcaga agatgacact tatctcgatg atgaagatgc agactggaaa acgacaaccc
      721 aggggcagaa atccgttggt gatacttcgg cgacgctggc ctggcgtccg ggtgacagcg
      781 ggcagaaaaa actggttcag ttgttcgact ccggtgaagt ctgcgcgttt cgtatcaaat
      841 atcccaacgg cactgttgat gttttccgcg gctggctgag ctcactgggt aaaaccattg
      901 cctcaaaaga cgtgatgacc cgcactgtga aaatcagcgg tgtggggcgt ccgtatctgg
      961 cagaggaagg cactgaaacc gtgggtgtta ccggactgac ggtggcaccg gcatctgtca
     1021 gtgtcaaagc gggagcaacc accacactga cctttacagt aaaacctgac ggggccagtg
     1081 acaaagcgat cagtgtgcat tcgtcagatc ctcagactgc ttcggtgacc ctgagcgggc
     1141 ttgtggccac ggtgaaaggc gtgaagcagg gcagtgtcag catcgtgggc atgacctctg
     1201 acggagagtt tgtggcagtg gctgcggtga ccgtcagcgc accataacag gacgatactc
     1261 atcattgccc cggttatccg gggctttttg catccggaga acatgatgtt tctgaaacag
     1321 gatacgttta attatgaaaa acagtccgtg gtgctcagtg agctgtccgg gctgcagaga
     1381 attgaatatc tgacgtttgt tcagcagcga acggcaaagt ttgatgcaca ggagggagaa
     1441 ctgccggagg ctgaacgaca gattgctttt ctgcgtatgg gaatggatat caatgcctgg
     1501 ctggtttccc gctcactgtg gaatgctgag cagtctcagg atgttgagac gctttgcgca
     1561 tccattatga caacatggtc gtatgatgcg ctgggcgcgg gggcggagag ggttctgtcg
     1621 ctgagcggta tggggaccat tgagaatgcc ggggatgatg atcatgaggc gctgacgccg
     1681 gaaaagtcct gacgcgggaa atgcagtttg tcatgcggct tgcccgggag ttccggcggg
     1741 cagactggcg gcggatgctg tcggatatgt cggccactga gcttggtgag tggggcgatt
     1801 atttccggat gcagagcttc agtgatgtgt ggatggatgc gcagtttgcc tcgctgaagg
     1861 cattgatcgt gagaatggtg tccggcagca gcgatgctgc ggtggctgat ttcagccttt
     1921 taccggaaga gaacgggata ccggagcgaa cggacgaaga actgatgcat cttggggaag
     1981 gtatttccgg aggtgtgcgt tatggaccag atagccaacc tggtcattga tttggggatt
     2041 gatgcggcag agtttaaaaa tgaaattccc cgtatcaaaa accttctgaa tggtgcagcc
     2101 agcgatgcag aacggtcttc tgcccgtatg cagcgtttta tggagcgtca gactcaggcg
     2161 gcccggcaga caatgcaggc ggcttcttcg gctgcaacag cagcatcagc ccatgcgcag
     2221 acggtggaga agaatgcacg ggctcatgaa cgcatggccc gtgaggtgga acaaacccgt
     2281 ctgcgcgtgg atgccctgaa tcagaaaatg cgcgaggaac aggcgcaggc cagggcactg
     2341 gcggaggcgc aggataaagc ggctgccgcc ttttatcgcc agattgacag tgtgaaacag
     2401 gccggtgcgg ggcttcagga attacagcgt attcagcagc agatccgaca ggccagaaac
     2461 agtggcgggg ttggtcagca ggattatctg gcgctgattt cggagatcac ggcgaaaacc
     2521 cgtgccctga cgcaggcaga ggaacaggcc acccggcaga aagcggcgtt tatccgccag
     2581 cttaaagagc aggcaacccg ccagaatctg tcgtcttctg agttgcttcg cgccagggcg
     2641 gctcagctgg gggtaagcag tgctgcagaa gtgtatatcc gcaaaatgga gcgggcagga
     2701 aaagccacac attcgctggg gctgaaaagt gcggcagccc ggcgggagct gggggtgtta
     2761 atcagtcaga tggcgcgagg caattttggt gcgctgaggg gatccgggat aacgctggct
     2821 aaccgtgccg gatggacagg cgcactgatg tcgccgaaag gcatgatgac tggcggcgtt
     2881 attggcggac ttgtcgcggc ggtcctgggt ctgggtaaag cctggcatga cggccggaag
     2941 gagggcgaga aatttaaccg tcagctggcg ctgacgggac attatgccgg tgtcactgtc
     3001 gggcagttgt ggaaactcag ccgggccata tccgggaatg gtatcacgca acatgcggca
     3061 gccggtgcgc tggcacaggt ggtgggcagt ggggcatttc atggaaacga tattggtatg
     3121 gtggcgaaag ctgccgcaca gatggagcga tcggttggcc agtcggtcag cgataccata
     3181 aatcagttta agcggctgaa ggatgatcct gtaaatgccg cgaaggcgct ggacaatgcg
     3241 ctgcattttt tgaatgccac tcagcttgag cagatacgcg tccttgggga gcaggggcgg
     3301 tccagtgatg cggcccggat cgccatgtct gcgctggcag aggaaaccgg taaacgcaca
     3361 tctgatattg ataataatct caatgcgctg ggtagtacgc tgcaaacctt gtctgactgg
     3421 tggaagcagt tctgggatgc ggccatgaat attggtcgtg aagactcgct ggatgcgcag
     3481 attgatgcgt tacaggaaaa aattcagcgc gcgaaaaaat atccgtggac aaacgcctcc
     3541 acacaggtgg agtacgatca gcagcgtctt aacgatcttc aggagaaaaa acgccggaag
     3601 gatttgcagg atgcaaaagc gcaggcagaa cggaattacc aggagcaaca gaaacgccgg
     3661 aatgctgaaa atgccgcgct gaaccggatg aatgaaacgg aagctgcacg acatcagcgg
     3721 gaaattgcgc gtattaatgc catgcagtac gccgaccagg ctgtcaggga tgcggcgata
     3781 cagcgtgaaa atgaacgtta cgaaaaagcc attaagaaaa atacacgggc aacccgtaat
     3841 gatgaggcca cccggttatt gctgcagtac agtcagcagc aggcacaggt ggaagggcag
     3901 attgctgctg ccagacagtc agcaggcatt gccactgaaa ggatgactga agcgcataaa
     3961 cagcttctgg ccctgcagca gcgcatcagc gacctggacg ggaaaaagct gacggcagat
     4021 gaaaagagtg tgctggcccg taagaatgag ctgattcagg cgctgacgct gctggatgtg
     4081 aaacagcagg agctgcagaa acagacagcg cttaacgacc tgagaaaaaa aacggttcag
     4141 ctgaccagcc agctggcaga caaagaacgt gcactgcgtg agcagcacaa tctggatatt
     4201 gccactgcag gtatggggga taagcagcgg cagcgctacc aggcacagtt gcgcatccgg
     4261 caggaatacc ggcaacagtt gcaacagctt gagaatgaca gtcgccagaa aggcacttac
     4321 gggacggagg actaccggag ggctgaggag gtgctgaagg ggagcctgaa gcgacaactg
     4381 aatgaaaaca aacgctactg gcaggaactg gaagtggcgc agggcgactg gaaaaacggt
     4441 gccatgcggg cgtttcagaa ttttacggcg gatgcggata atgcggcggg aacggcagaa
     4501 cagatgttta cagtggcatt cagcagtgcc ggtaatgcac tggcgacatt ctgtaccacc
     4561 ggtaagctga attttaaatc cttcacctct tccctgttgt cagatatggc cagaattatg
     4621 gcacagatgg ccatgatgca ggcggtaaag ggcgtcggtt ctttattcgg cttcacgact
     4681 aatgctgatg gcggtgttta ccagtctgct gatttgagtc gctacagtgg cacggtggtt
     4741 aaccgtccga cgttttttgc ttttgcaaaa ggcgcgggtg tgatggggga agcgggacct
     4801 gaagccattc tgccactgcg tcgcggtgct gatggtaagc tgggggttgt ggcggatact
     4861 ggtggttcag gcatggtgat gtttgccccg cagtacaaca ttgagatcaa taacgacggc
     4921 acgaacgggc agataggtcc ggctgccctg aaggtggttt atgacctcgg gaaaaaagcg
     4981 gccgcggact ttatgcaaca gcaggcccgt gatggtggcc ggttaagtgg agcataccgg
     5041 taatggagac gtttcactgg aaagtgcgcc cggatatgaa tgtggtatca gagccgaaag
     5101 tggtgacagt gaagctgggc gatggttatg aacagcgtcg tgcggcggga ctgaataacc
     5161 agttgtcgac ttacagcgtg acgatacgtg ttcgtaaagg tgaacaccca tctttaaaag
     5221 cctttctgga acggcacggt ggcgtccgcg catttcagtg gacgccacct tatgactgga
     5281 aactgatcag ggtggtttgt cgtaaatggt cggcaagcgt gggggcgtta tgggtgactg
     5341 taacggccga ttttgaacag gtggtgaact gatgcaggat atccggcagg aaacactgaa
     5401 tgaatgcacc cgtgcggagc agtcagcccg ggtggagctc tgggaaatcg atctgacaga
     5461 ggtcggtggt gagcgttatt ttttctgtaa tgagcagaac gaaaaaggtg agccggtcac
     5521 ctggcagggg cggcagtatc aggcataccc cattcagggg tcggggtttg aactgaacgg
     5581 caggggctgt gctgcccgtc cgacactgac ggtctctaac ctgcacggca tggtcaccgg
     5641 gatggcggaa gatctgcaga gtctggtcgg cggaacggtg gtccggcgta aggtttacgc
     5701 ccgttttctg gatgcggtga acttcgtcaa cggaaacagc gacgccgatc cggaacagga
     5761 ggtgatcagc cgctggcgca tcgagcagtg cagcgaactg agcgcggtca gtgcctcttt
     5821 tgtgctggcc acaccaacgg agacggatgg cgcggttttc ccggggcgta tcatgctggc
     5881 gaatacctgt atgtggactt accgttctga tgagtgtggt tacacgggca gggcagtggc
     5941 tgacgagttc gacaaaccaa caacggatat ccggaaggac aaatgcagca agtgtatgcg
     6001 cgggtgtgag ttgcgcaaca ataccggtaa tttcggcggt ttcctttcca tcaataaact
     6061 ttctcagtaa atccatgaca cagacagaat cagcgattct ggcgcacgcc cggcgatgtg
     6121 cgccagcgga gtcgtgtggc ttcgtggtga gaacgccgga gggagacagg tatcttccca
     6181 gcgagaatat ctccggtgag ccggaggaac ggttccggat ggctccggag gactggctgc
     6241 gggcacaaat gcagggtgag attgtggcac tggtccacag tcatcccggt ggtctgccct
     6301 ggctgagtga ggctgaccgg cggctgcagg tgcagagtga tttgccgtgg tggctggtct
     6361 gccgggggac gattcacaag ttccgctgtg tgccgcatct caccgggcgg cgctttgagc
     6421 acggggtgac ggactgttac acgctgttcc gggatgctta tcatctggcg gggattgaga
     6481 tgccggattt tcatcgcggg gatgactggt ggcgtcacgg tcagaatctc tatctggata
     6541 atctggaggc cacagggctg tatcaggtgc cgttgtcatc agcacaaccg ggcgatgtgc
     6601 tgctgtgctg ttttggttca tcggtgccga atcatgccgc catttactgc ggcgatggtg
     6661 agctgctgca ccatattcct gaacaactga gtaaacgaga gaggtatacc gacaaatggc
     6721 agcgacgcac acactccctc tggcgtcacc gggaatggca cgcatctgcc tttacgggga
     6781 tttgcaacga tttggccgcc gcatcgacct tcgtgtgaaa acgggggctg aagccatccg
     6841 cgcactggcc acacagctcc cggcgtttcg tcagaaactg agtgacggct ggtatcaggt
     6901 gcgcattgcc gggcgtgatg caggtgaaac cgaattgtct gcccgtctta atgagccgct
     6961 ggcaaatggt gcagtgatcc acatagtacc gcgtctggtg ggagctaaaa gtggcggtgt
     7021 gtttcaggcg gtgctggggg cagctgttat ggcggttgct atatggatgc cgggggtagg
     7081 aattatggcg agtaatctgc tgttttctct cggtgccagt atgacgcttg gcggtgttgc
     7141 acagatgctg gcaccgaaag ccagaactcc ccgtacacag acaacggata acggcaaaca
     7201 gaacacctgt ttctcctcac tggataacat ggttgcccag ggcaatgttc tgcctgttct
     7261 gtacggtgaa atgcgcgtgg ggtcgcgggt ggtatctcag gagatcagca cggcagacga
     7321 aggggatggt ggtcaggttg tggtgattgg tcgctgatgc aaaatgtttc atgtgaaacc
     7381 gcctgcgggc ggttttgtcg tttatggagc gtgaggaatg ggtaaaggca gcagtaaggg
     7441 gcataccccg cgcgaagcga aggacaacct gaaatccacg cagttgctga gtgtgattga
     7501 tgccatcagc gaagggccgg ttgacggtcc ggtggatgga ttaaaaagcg tgctgctgaa
     7561 cggtacgccg gtcctggata gcgaggggaa gacaaacttt tccggtgtta cggtggtgtt
     7621 ccgcgccggc gagcaggagc agacaccgcc ggaggggttt gaatcttccg gctcagagac
     7681 tgtgctgggt acggaagtga aatacgacac gccgatcacc cggaccatca cgtcggcaaa
     7741 cattgatcgt ctgcgcctga ccttcggtgt gcaggcactg gtggaaacca cctcaaaggg
     7801 ggaccggaat ccgtcggaag tccgcctgct ggttcagata cagcgtaacg gtggctgggt
     7861 gacggaaaaa gacatcacca ttaagggcaa aaccacttcg cagtatctgg cctcggtggt
     7921 ggtgggtaac ctgccgccgc gcccgtttaa tatccggatg cgcaggatga caccggacag
     7981 caccacagac cagctgcaga acaaaacgct ctggtcgtcg tacaccgaaa tcatcgatgt
     8041 gaaacagtgc tacccgaaca cggcgctggt cggcgtgcag gtggattcag agcagttcgg
     8101 taaccagcag gtgagtcgca attatcatct tcgcgggcgc attctgcagg tgccgtcgaa
     8161 ctataacccg cagacgcggc aatacagcgg tatctgggac ggaacgctta agccagcata
     8221 cagcaacaac atggcctggt gtctgtggga catgctcact catccgcgct acggcatggg
     8281 gaaacgtctt ggtgcggcag atgtggacaa atgggcgctg tatgtcatcg gccagtactg
     8341 cgaccagtca gtgccggacg gatttggcgg cacggagccg cgcatcacct gtaacgctta
     8401 cctgaccaca cagcgtaagg cgtgggatgt tctcagtgat ttctgctcgg cgatgcgctg
     8461 tatgccggta tggaacgggc agacgctgac gttcgtgcag gaccgaccgt cggataaagt
     8521 gtggacctat aaccgcagta atgtggtgat gccggatgat ggcgcgccgt tccgctacag
     8581 cttcagcgcc ctgaaggacc gccataatgt cgttgaggtg aactggattg atccggacaa
     8641 cggtcatgag acggcgacag agcttgtgga ggacacgcag gccattgtcc gttacggtcg
     8701 taacgtcacg aagatggatg cctttggctg taccagccgg ggacaggcgc accgcgccgg
     8761 gctgtggctg attaaaacgg aactgctgga aacgcagacc gtggacttca gcgtcggcgc
     8821 agaagggctt cgccatgtac cgggtgatgt cattgaaatc tgcgatgatg attatgccgg
     8881 tatcagcacc ggtggtcgcg tactggcggt gaacagccag acccggacgc tgacgctcga
     8941 ccgtgaaatc acgctgccgt cctccggtac cacgctgata agcctggttg acggaaatgg
     9001 caatccggtc agcgtggagg ttcagtccgt caccgacggc gtgaaggtga aagtgagccg
     9061 ggttcctgac ggcgttgcag aatacagcgt gtgggggctg aagctgccga cgctgcgcca
     9121 gcgcctgttc cgctgcgtga gtatccgtga gaacgacgac ggcacgtatg ccatcaccgc
     9181 cgtgcagcat gtgccggaaa aagaggccat cgtggataac ggggcacact ttgacggtga
     9241 ccggcgcggc acggtgaatg gtgtcacgcc gccagcggtg cagcatctga ccgccgaagt
     9301 caccgcagac agcggggaat atcaggtgct ggcgcgatgg gacacgccga aggtggtgaa
     9361 gggtgtgagc ttcctgcttc gcctgaccgt ggcagcggac gacggcagtg agcggctggt
     9421 cagcacagcc aggacgacgg aaaccacata ccgcttcagg cagctggcgc tggggaatta
     9481 cagtctgaca gtccgggcgg taaatgcccg ggggcagcag ggcgatccgg cgtcggtatc
     9541 gttccggatt gcggcaccgg cagcgcctgt cactattgaa ctgataccgg ggtattttca
     9601 gataacggtg gtcccgaaac tggctgtata tgacccgacg gtgcagtttg agttctggtt
     9661 ttcggaaaag cggattgcgg atatcaggca ggttgaaacc agcgcccgct atcttggcac
     9721 ggcgctgtac tggatagccg ccagtatcaa tatcaggccg ggccatgatt attattttta
     9781 cgttcgcagt gtgaacaccg ttggcaaatc ggcatttgtg gaggccgtcg gtcgggcgag
     9841 cgatgatgcg gaaggttatc tgagttttta taaagggttg atcaataaaa cgcatctcgg
     9901 caaggagttg tggacgcaga ttgataacgg tcagcttgcg ccggacctga ctgaaatcag
     9961 gacgtccatt acgaatgtca gcaatgaaat cacgcaaacc gtcaataaaa aactggaaaa
    10021 tcagagtgct gcaatccagc agatacagaa agttcaggtt gatacaaata ataacctgaa
    10081 cagcatgtgg gctgtgaagc tgcaacagat gaaggacgga cgcctttata ttgcgggtat
    10141 cggtgccggt attgagaata cgccagcagg aatgcagagt caggtgctgc tggcggcaga
    10201 caggattgcg atgattaatc ctgcgaatgg caacacaaag ccgatgtttg ttggtcaggg
    10261 cgatcagata ttcatgaacg acgtgttcct gaaacgcctg acggctccga ccattaccag
    10321 cggcggtaat cctccggcat tttccctgac acctggcgga cggctgacgg cgaaaaatgc
    10381 cgatatcagc ggtaacgtga acgcgaactc cgggacgctc aacaacgtca cgattaataa
    10441 gaactgtcgg gttctgggaa aattgtccgc gaaccagatt gaaggcgatc tcgttaaaac
    10501 agtgggcaaa cctttcccac gggactcccg ggcaccggag aggtggccat cagggaccat
    10561 taccgtcagg gtttatgacg atcagccgtt tgatcggcaa attgttattc ccgcggtggc
    10621 gtttcgcggt gctaaacatg agcggaagaa taacaatatt tattcgtcat gccgcctgat
    10681 agtgaagaaa aacggtgctg aaatttataa ccgaacgacc ctggataata cgctgatata
    10741 tacgggtgtt attgatatgc ctgccggtca cggtcacatg acgctggagt tttctgtatc
    10801 ggcatggctg gtaaatggct ggtatcccac agcaagtatc agcgatttgc tggttgttgt
    10861 gatgaagaaa tccacagcag gtatcagtat cagctgaatt ttataaccca gatacgggca
    10921 ccagaaatgg tgcctttttt attgcagaaa agcgagaggt aattatgcgt aaactttatg
    10981 ctgccatttt gtccgcagcc atctgtctgg ctgtatccgg tgcgcctgca tgggcgtctg
    11041 aacatcagtc cacgctgagc gcggggtatc ttcatgcctc gacgaacgct cccggcagtg
    11101 atgatctgaa cgggattaac gtgaaatacc gttatgagtt tacggacacg ctggggctgg
    11161 tgacgtcatt cagctatgcc aacgctgaag atgagcaaaa aacgcattac agtgataccc
    11221 gctggcatga ggattccgtg cgtaaccgct ggttcagcgt gatggcgggg ctgtctgtgc
    11281 gcgtgaatga atggttcagc gcgtatgcga tggcgggcgt ggcttacagc cgtgtgtcga
    11341 ccttctccgg ggattatctc cgcgtaactg acaacaaggg gaaaacgcac gatgtgctga
    11401 ccgggagtga tgacaaccgc cacagcaaca cgtctctggc gtggggggct ggcgtgcagt
    11461 ttaacccgac cgaatccgtg gccattgacc ttgcttatga aggttccggc agtggcgact
    11521 ggcgcactga cggtttcatc gtgggtgtcg gttataagtt ctgattagcc aggtaacaca
    11581 gtgttatgac agcccgctgg ttcaggcggg ctttttgtgg ggtgaatatg gcagtaaaga
    11641 tttcaggtgt actgaaagat ggggcgggta aacctgtcgt aaattgtgcg attgaactgc
    11701 gggccagaag aaccagtccg accgttgtgg cacacgttgt tgccacttgc gtgacggaca
    11761 ataacggtgc ttatgtgatt gaggctgagc cggggtatta cgaggttgcg cttcactgta
    11821 acggctggca gccaacccgt gtcggggata ttgatgtggc accgactgat gcaccgggga
    11881 cactgaacgc gtttctgaat gcaccaaagg atggtgattt acgtccggag gtgatgaagc
    11941 gctttgagga aatggtggcg caggcgcagc agagtgccgg ggctgcagcc ggaaacgcac
    12001 agcagacggc gcaggatgtg gcggcagccg caacggcccg tgatgatgca caacgttttg
    12061 cggagaaagc ccgacaggat gcaaccgtca cagctgagga cagaaaggcc actgcggaag
    12121 atgtgacaag cacaggagca aatgcagccg cagccggaca gagcgcacag gatgccgcag
    12181 gttatgcccg cgcagcagaa caggccaaaa atgacattga tgctgcgctg accggcactc
    12241 tgaaaatggc taaccatctg tc
//
LOCUS       DAJTRC010000105        12220 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.105, whole genome shotgun sequence.
ACCESSION   DAJTRC010000105 DAJTRC010000000
VERSION     DAJTRC010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12220)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12220)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12220
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.105"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..493)
                     /locus_tag="OO357_003267"
     CDS             complement(<1..493)
                     /locus_tag="OO357_003267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5888108.1"
                     /translation="MKIPTTTDIPQRYTWCLAGICYSSLAILPSFLSYAESYFNPAFL
                     LENGTSVADLSRFERGNHQPAGVYRVDLWRNDEFIGSQDIVFESTTENTGDKSGGLMP
                     CFNQVLLERIGLNSSAFPELAQQQNNKCINLLKAVPDATINFDFAAMRLNITIPQIAL
                     LSSA"
     gene            complement(524..1216)
                     /gene="fimC"
                     /locus_tag="OO357_003268"
     CDS             complement(524..1216)
                     /gene="fimC"
                     /locus_tag="OO357_003268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5888109.1"
                     /translation="MMTKIKLLMLMIFYLIISASAHAAGGIALGATRIIYPADAKQTA
                     VWIKNSHTNELFLVNSWIENSSGVKEKSFIITPPLFVSEPKSENTLRIIYTGPPLAAD
                     RESLFWMNVKTIPSVDKNALNGRNVLQLAILSRMKLFLRPIQLQELPAEAPDTLKFSR
                     SGNYINVHNPSPFYVTLVNLQVGSQKLGNAMAAPRVSSQIPLPSGVQGKLKFQTVNDY
                     GSVTPVREVNLN"
     gene            complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="OO357_003269"
     CDS             complement(1436..1978)
                     /gene="fimA"
                     /locus_tag="OO357_003269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308619.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5888110.1"
                     /translation="MKLRFISSALAAALFAATGSYAAVVDGGTIHFEGELVNAACSVN
                     TDSADQVVTLGQYRTDIFNAVGNTSALIPFTIQLNDCDPVVAANAAVAFSGQADAIND
                     NLLAIVSSTNTTTATGVGIEILDNTSAILKPDGNSFSTNQNLIPGTNVLHFSARYKGT
                     GTSASAGQANADATFIMRYE"
     gene            2459..3325
                     /gene="folD"
                     /locus_tag="OO357_003270"
     CDS             2459..3325
                     /gene="folD"
                     /locus_tag="OO357_003270"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HCR5888111.1"
                     /translation="MAAKIIDGKTIAQQVRSEVAQKVQARIAAGLRAPGLAVVLVGSN
                     PASQIYVASKRKACEEVGFVSRSYDLPETTSEAELLELIDALNADNTIDGILVQLPLP
                     AGIDNVKVLERIHPDKDVDGFHPYNVGRLCQRAPRLRPCTPRGIVTLLERYNIDTFGL
                     NAVVIGASNIVGRPMSMELLLAGCTTTVTHRFTKNLRHHVENADLLIVAVGKPGFIPG
                     DWIKEGAIVIDVGINRLENGKVVGDVVFEDAAKRASYITPVPGGVGPMTVATLIENTL
                     QACVEYHDPQGE"
     gene            3327..3539
                     /gene="ybcJ"
                     /locus_tag="OO357_003271"
     CDS             3327..3539
                     /gene="ybcJ"
                     /locus_tag="OO357_003271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308617.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated protein YbcJ"
                     /protein_id="HCR5888112.1"
                     /translation="MATFSLGKHPHVELCDLLKLEGWSESGAQAKIAIAEGQVKVDGA
                     VETRKRCKIVAGQTVSFAGHSVQVVA"
     gene            3647..4156
                     /locus_tag="OO357_003272"
     CDS             3647..4156
                     /locus_tag="OO357_003272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5888113.1"
                     /translation="MPTVITHAAVPLCIGLGLGSKGLLFAGIILAMLPDADVLSFKFG
                     VAYGNVFGHRGFTHSLVFAFVVPLLCVLIGRRWFRAGLIRCWLFLTVSLLSHSLLDSV
                     TTGGKGVGWLWPWSDERFFAPWQVIKVAPFALSRYTTAYGHQVIISELMWVWLPGMLL
                     MGMLWWRRR"
     gene            complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="OO357_003273"
     CDS             complement(4192..5577)
                     /gene="cysS"
                     /locus_tag="OO357_003273"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HCR5888114.1"
                     /translation="MLKIFNTLTRQKEEFKPIHAGEVGMYVCGITVYDLCHIGHGRTF
                     VAFDVVARYLRFLGYKLKYVRNITDIDDKIIKRANENGESFVALVDRMIAEMHKDFDA
                     LNILRPDMEPRATHHIAEIIELTEQLIAKGHAYVADNSDVMFDVPTDPTYGVLSRQDL
                     DQLQAGARVDVVDDKRNRMDFVLWKMSKEGEPSWPSPWGAGRPGWHIECSAMNCKQLG
                     NHFDIHGGGSDLMFPHHENEIAQSTCAHDGQYVNYWMHSGMVMVDREKMSKSLGNFFT
                     VRDVLKYYDAETVRYFLMSGHYRSQLNYSEENLKQARAALERLYTALRGTDKTVAPAG
                     GEAFEARFIEAMDDDFNTPEAYSVLFDMAREVNRLKAEDMAAANAMASHLRKLSAVLG
                     LLEQEPEAFLQSGAQADDSEVAEIEALIQQRLDARKAKDWAAADAARDRLNEMGIVLE
                     DGPQGTTWRRK"
     gene            5751..6245
                     /gene="ppiB"
                     /locus_tag="OO357_003274"
     CDS             5751..6245
                     /gene="ppiB"
                     /locus_tag="OO357_003274"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017465360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase B"
                     /protein_id="HCR5888115.1"
                     /translation="MVTFHTNHGDIVIKTFDDKAPETVKNFLDYCREGFYNNTIFHRV
                     INGFMIQGGGFEPGMKQKATKEPIKNEANNGLKNTRGTLAMARTQAPHSATAQFFINV
                     VDNDFLNFSGESLQGWGYCVFAEVVDGMDVVDKIKGVATGRNGMHQDVPKEDVIIESV
                     TVSE"
     gene            6248..6970
                     /gene="lpxH"
                     /locus_tag="OO357_003275"
     CDS             6248..6970
                     /gene="lpxH"
                     /locus_tag="OO357_003275"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase"
                     /protein_id="HCR5888116.1"
                     /translation="MATLFIADLHLCVEEPAITAGFLRFLAGEARKADALYILGDLFE
                     AWIGDDDPNPLHRQMAAAIKAVSDSGVPCYFIHGNRDFLLGKRFARESGMTLLPEEKV
                     LELYGRRVLIMHGDTLCTDDAGYQAFRAKVHKPWLQMLFLALPLFVRKRIAARMRANS
                     KEANSSKSLAIMDVNQNAVVSAMEKHQVQWLIHGHTHRPAVHELIANQQPAFRVVLGA
                     WHTEGSMVKVTADDVELIHFPF"
     gene            7088..7597
                     /gene="purE"
                     /locus_tag="OO357_003276"
     CDS             7088..7597
                     /gene="purE"
                     /locus_tag="OO357_003276"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706401.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HCR5888117.1"
                     /translation="MSSRNNPARVAIVMGSKSDWATMQFAVEIFEILNVPHHVEVVSA
                     HRTPDKLFSFAESAEENGYQVIIAGAGGAAHLPGMIAAKTLVPVLGVPVQSAALSGVD
                     SLYSIVQMPRGIPVGTLAIGKAGAANAALLAAQILATHDKELHQRLNGWRKAQTDEVL
                     ENPDPRGAA"
     gene            7594..8661
                     /gene="purK"
                     /locus_tag="OO357_003277"
     CDS             7594..8661
                     /gene="purK"
                     /locus_tag="OO357_003277"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HCR5888118.1"
                     /translation="MKQVCVLGNGQLGRMLRQAGEPLGIAVWPVGLDAEPAAVPFQQS
                     VITAEIERWPETALTRELARHPAFVNRDVFPIIADRLTQKQLFDKLHLPTAPWQLLAD
                     RSEWPAVFDRLGELAIVKRRTGGYDGRGQWSLRANETEQLPAECYGECIVEQGINFSG
                     EVSLVGARGFDGSTVFYPLTHNLHQDGILRTSVAFPQANAQQQAQAEEMLSAIMQELG
                     YVGVMAMECFVTPQGLLINELAPRVHNSGHWTQNGASISQFELHLRAITDLPLPQPVV
                     NNPSVMINLIGSDVNYDWLKLPLVHLHWYDKEVRPGRKVGHLNLTDSDTSRLTATLEA
                     LIPLLPPEYASGVIWAQSKFS"
     gene            complement(8798..9691)
                     /locus_tag="OO357_003278"
     CDS             complement(8798..9691)
                     /locus_tag="OO357_003278"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HCR5888119.1"
                     /translation="MKTLVVALGGNALLQRGEALTAENQYRNIASAVPALARLARSYR
                     LAIVHGNGPQVGLLALQNLAWKEVEPYPLDVLVAESQGMIGYMLAQSLSAQPQMPPVT
                     TVLTRIEVSPDDPAFLQPEKFIGPVYQPEEQEALEAAYGWQMKRDGKYLRRVVASPQP
                     RKILDSEAIELLLKEGHVVICSGGGGVPVTEDGAGSEAVIDKDLAAALLAEQINADGL
                     VILTDADAVYENWGTPQQRAIRHATPDELAPFAKADGSMGPKVTAVSGYVRSRSKPAW
                     IGALSRIEETLAGEAGTCISL"
     gene            complement(9688..10335)
                     /locus_tag="OO357_003279"
                     /pseudo
     CDS             complement(9688..10335)
                     /locus_tag="OO357_003279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308609.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
     gene            10338..11570
                     /gene="allC"
                     /locus_tag="OO357_003280"
     CDS             10338..11570
                     /gene="allC"
                     /locus_tag="OO357_003280"
                     /EC_number="3.5.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoate deiminase"
                     /protein_id="HCR5888120.1"
                     /translation="MTHSRQAIEETLPWLSSFGADPTGGMTRLLYSPEWLETQQQFKK
                     RMAASGLETRFDEVGNLYGRLSGTEYPQEVVLSGSHIDTVVNGGNLDGQFGALAAWLA
                     IDWLKTQYGAPLRTVEVVAMAEEEGSRFPYVFWGSKNIFGLANPDDVRNICDAKGNSF
                     ADAMKACGFTLPNAPLTPRQAIKAFVELHIEQGCVLESNGQSIGVVNAIVGQRRYTVT
                     LNGESNHAGTTPMGYRRDTVYAFSRICHQSIEKAKKMGDPLVLTFGKVEPRPNTVNVV
                     PGKTTFTIDCRHTDAAVLRDFTQQLENDMRAICDEMDIGIDIDLWMDEEPVPMNKELV
                     ATLTELCEREKLNYRVMHSGAGHDAQIFAPRVPTCMIFIPSINGISHNPAERTNITDL
                     AEGVKTLALMLYQLAWQE"
     gene            11581..>12220
                     /gene="allE"
                     /locus_tag="OO357_003281"
     CDS             11581..>12220
                     /gene="allE"
                     /locus_tag="OO357_003281"
                     /EC_number="3.5.3.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(S)-ureidoglycine aminohydrolase"
                     /protein_id="HCR5888121.1"
                     /translation="MGYLNNVTGYREDLLANRAIVKHGNFALLTPDGLIKNIIPDFEN
                     CDATILSTPKLGASFVDYLVTLHQNGGNQQGFGGEGIETFLYVISGNITAKAEGKTFA
                     LSEGGYLYCPPGSLMTFVNAQAEDSQIFLYKRRYVPVEGHAPWLFSGNASELERIHYE
                     GMDDVILLDFLPKELGFDMNMHILSFAPGASHGYIETHVQEHGAYILSGQGVY"
BASE COUNT         2966 a         3158 c         3118 g         2978 t
ORIGIN      
        1 gagcgctact caacaacgct atctgaggaa tagtgatgtt caggcgcatc gctgcaaaat
       61 caaagttaat tgtggcatca ggtacagctt tcagtaaatt gatgcattta ttgttttgct
      121 gctgggctaa ctcgggaaat gcactgctat taaggccaat tcgttcaagg agtacctggt
      181 taaaacaggg cattaaccca cctgatttat cacctgtatt ttctgttgtc gattcaaata
      241 cgatatcctg tgaaccaatg aactcatcat tacgccagag atccacccga tacacgcccg
      301 caggttgatg attacctctc tcaaagcgcg ataaatcagc aacggatgtg ccattctcta
      361 ataaaaatgc cgggttgaaa taactttccg catagcttaa aaaggagggt aaaatggcaa
      421 gagatgaata acaaattccg gccagacacc aggtatacct ctgcggaata tccgtagtag
      481 tgggtatttt cataattagc tctgatattg tcagatgatt cggttagttt aagttcactt
      541 ctctgaccgg ggttactgaa ccataatcat taacggtctg aaatttcagc tttccctgca
      601 ctcctgaggg taagggaatt tgtgaactaa ctctgggtgc cgccatagca ttccccaact
      661 tttggctgcc cacttgcagg ttaacgaggg tgacataaaa aggtgatgga ttatgaacat
      721 tgatatagtt accggatcgc gaaaacttga gtgtgtccgg cgcttctgcg ggtaattctt
      781 gtaattgaat tggacggaga aataatttca tgcgcgataa aatcgccagt tgcaaaacat
      841 tcctgccgtt caatgcattt ttatctaccg aagggatcgt cttaacattc atccagaaca
      901 gagactcacg atctgccgcc agcggtggac cggtgtaaat aatacgcaaa gtattttcgc
      961 ttttgggttc actaacaaat agtggcggtg taatgatgaa tgacttttct tttacaccgc
     1021 tgctgttttc aatccacgaa ttgaccagaa agagctcatt ggtatggcta tttttaatcc
     1081 ataccgcagt ctgtttagca tcagcgggat aaataatacg cgtggcacct aatgcgatcc
     1141 ctccggcagc atgggcgctg gccgaaatga ttaaataaaa tatcatgagc atcaataact
     1201 ttattttagt catcatagtt aatacgcctt gtgtattcct gacggcaaaa ataaggagta
     1261 taaagaaatg attgctttgg cttcttacaa cacatcattc ccttttcatt taataaagcg
     1321 gcatgataca aagtgaacgt ggtttcgatt tacattgcca ccataccgct tcagacaacc
     1381 ggtacattac tttgttataa gacgtgatat ataattcaaa acaacgtggt tttgattatt
     1441 cgtatctcat aataaaagtc gcgtcagcat ttgcttgccc tgctgatgca cttgtaccgg
     1501 tgcctttata acgtgcagaa aaatgaagaa cgttggtccc ggggatcaag ttctggttgg
     1561 ttgagaagct attcccatca ggtttgagaa ttgcggatgt attatcaagt atttcaatac
     1621 cgacacccgt tgctgttgtt gtatttgtac tggatacaat ggccaataaa ttatcattga
     1681 ttgcatcagc ctgaccagaa aatgcaacgg cagcattagc ggcaacaaca ggatcgcagt
     1741 cgttcaactg aatggtgaat ggaattaatg cagaggtatt accaacagca ttgaaaatat
     1801 cggtacgata ttgaccgagt gtgacaacct ggtctgccga gtcagtattc actgaacagg
     1861 cagcattcac cagttcgcct tcaaagtgaa ttgtaccgcc atctacaacg gcagcataac
     1921 tacccgtagc ggcgaatagt gcggcagcca gcgcagacga aataaatctt aatttcatat
     1981 atattccttc aatctcattt atcgactcca catccgtata taaccgatta ctttatttaa
     2041 gacactgata gtagtaaaat tcctttttat cctctaagaa tgtcttaatt gaaaatatgc
     2101 actctattct aaaaaataga gagccccgtt agatgaatac ttccgcgcaa aatatattca
     2161 ccacaaatat agacctgaag cggtaaatta ccaggctgaa aattcttttt atattgtcag
     2221 gtatttctta aattatctta atccttaaac aaggaaataa atcagttcca gatttacaac
     2281 gccatcatgt acgaaaaatg aagctttcaa tctcagcgac ctcagcgacg gtgcgcctaa
     2341 ccttcgcaag aggtcgcttc acgcgataaa tctgaaacga aacctgacag cgcgccccgc
     2401 ttctgacaaa atagacgcat ccccttcgac ctacgtaaca gatggaatcc tctctctgat
     2461 ggcagcaaag attattgacg gtaaaacgat tgcgcagcag gtgcgctctg aagttgctca
     2521 aaaagttcag gctcgtattg cagccggact gcgggcacca ggactggccg ttgtgctggt
     2581 gggtagtaac cctgcatcgc aaatttatgt cgcaagcaaa cgcaaggctt gtgaagaagt
     2641 cgggttcgtc tcccgctctt atgacctccc ggaaaccacc agcgaagcgg agctgctgga
     2701 gcttatcgat gcgctgaatg ccgacaacac catcgatggc attctggttc aactgccgtt
     2761 accggcgggt attgataacg tcaaagtgct ggaacgtatt catccggaca aagacgtgga
     2821 cggtttccat ccttacaacg tcggtcgtct gtgccagcgc gcgccgcgtc tgcgtccctg
     2881 caccccgcgc ggtatcgtca cgctgcttga gcgttacaac attgatacct tcggcctcaa
     2941 cgccgtggtg attggcgcat cgaatatcgt cggtcgccca atgagcatgg aactgttgct
     3001 cgcaggttgc accaccaccg tgactcaccg cttcactaaa aatctgcgtc atcacgtaga
     3061 aaatgccgat ctgttgatcg ttgccgttgg caagccaggc tttattcccg gtgactggat
     3121 caaagaaggc gcaattgtga ttgatgtcgg catcaaccgt ctggaaaatg gcaaagttgt
     3181 gggcgacgtc gtgtttgaag acgcggctaa acgcgcctca tacattacgc ctgttcccgg
     3241 cggcgttggc ccgatgacgg ttgccacgct gattgaaaac acgctacagg cgtgcgttga
     3301 atatcatgat ccacagggtg agtaacatgg cgacattttc tttaggtaaa catccgcacg
     3361 ttgagctgtg cgacttgctg aaactggaag gctggagcga aagcggcgcg caggcgaaaa
     3421 tcgcgattgc cgaaggccag gtgaaagtcg acggtgcggt tgaaacgcgc aaacgctgca
     3481 aaatcgtcgc cggtcagaca gtgagttttg caggacacag cgtacaggtt gttgcctgat
     3541 ccctttcccg gcctgtatca acaggccggt tctcttttca gacttttgcc ctctacgccc
     3601 cgttcatcca tgctgatagc atcgacattt tgccgaagcg accatcatgc caaccgttat
     3661 tacgcacgcc gccgttcccc tttgcattgg tttagggttg ggttcgaaag gtctgttatt
     3721 tgccggaatc atcctggcga tgctgcccga tgccgacgta ttgtcgttta aatttggcgt
     3781 tgcttacggc aatgtttttg gtcatcgcgg ttttacccat tcgctggtgt ttgcgtttgt
     3841 tgtcccgctc ttatgtgtgc tgattggacg tcgatggttc agggctgggc tgattcgctg
     3901 ctggctgttt ttaaccgtct cgttgctatc gcacagctta ctggattcgg taaccactgg
     3961 cggtaagggc gttggctggc tgtggccgtg gtcagatgaa cgctttttcg ctccctggca
     4021 ggtgattaaa gtcgcgccgt ttgcattgtc tcgttacacc acagcgtacg ggcatcaggt
     4081 gattatttcc gaattgatgt gggtgtggtt gccggggatg ctactgatgg gaatgttgtg
     4141 gtggcgcaga cgataaccgg atgcgaaaac tcgcatccgg caattgcgca attacttacg
     4201 acgccaggtg gtcccttgcg ggccatcttc cagcacgatc cccatctcgt taagacggtc
     4261 acgtgccgca tctgccgccg cccagtcttt cgctttacgg gcatccagac gctgttgaat
     4321 taacgcttca atctcagcca cttcgctgtc gtctgcctgc gcgccgcttt gcaggaacgc
     4381 ttccggttct tgctccagca ggcccaatac agcggaaagt ttacgcaggt gagatgccat
     4441 tgcattcgcc gctgccatat cttctgcttt cagacggttc acttcacgcg ccatatcaaa
     4501 cagcacggaa taggcttccg gggtgttgaa atcgtcgtcc atcgcttcaa taaagcgcgc
     4561 ttcaaacgct tcgccaccgg caggcgcaac ggttttatct gtgccgcgca gcgcagtgta
     4621 gaggcgctcc agcgccgcac gcgcctgctt caggttctct tcgctgtagt tcagctggct
     4681 gcgatagtgg cccgacatca ggaagtaacg cacggtttcc gcgtcgtagt atttcagcac
     4741 atcgcgcacg gtaaagaagt tacccagcga tttggacatc ttctcgcggt caaccatcac
     4801 cattccggag tgcatccagt agttcacata ctgaccatca tgggcacagg tggactgcgc
     4861 gatttcgttc tcgtggtgcg ggaacatcag gtctgaaccg ccgccgtgga tatcaaagtg
     4921 gttacccagc tgcttgcagt tcatggccga acattcaatg tgccagccag gacgacccgc
     4981 gccccacgga gacggccagc tcggttcgcc ctctttcgac atcttccaca gaacgaagtc
     5041 catccggttg cgtttgtcat caaccacgtc aacgcgcgcg ccagcctgca gctggtcgag
     5101 atcctgacgc gacagcacgc cataagttgg atcggtcggg acgtcgaaca tcacgtcgct
     5161 gttgtccgcc acataagcgt gacctttggc gatcagttgt tcagtgagtt caataatttc
     5221 tgcgatatgg tgcgtcgcgc gcggctccat atccgggcgc agaatgttca aagcatcaaa
     5281 atctttgtgc atttcggcga tcatgcgatc caccagcgcc acaaagcttt cgccattttc
     5341 attggcgcgt ttgatgattt tgtcgtcgat atcggtaatg ttgcgcacat acttcagttt
     5401 atagccgagg aaacgcagat agcgcgcaac cacgtcaaaa gcaacaaagg tacgcccgtg
     5461 accgatatga cagagatcgt aaacggtgat tccacacacg tacatgccga cttccccggc
     5521 gtgaataggc ttaaattcct ctttttggcg tgtcagagta ttgaagattt ttagcatcga
     5581 agattccgtt tagacatgtg tgggtaattg agttgcgtat aatacccata tttcccgcgc
     5641 gaatcagcat acattgcgtg atgatccgat cgtgcggtta tgctataaca ccaccctata
     5701 tatgacccga actgggttga agcaccaatc aaacggaaca ggatgcaaaa atggttactt
     5761 tccacaccaa tcacggcgat attgtcatca aaacttttga cgataaagca cctgaaacag
     5821 ttaaaaactt cctggactac tgccgcgaag gtttttacaa caacaccatt ttccaccgtg
     5881 ttatcaacgg ctttatgatt cagggcggcg gttttgaacc gggcatgaaa caaaaagcca
     5941 ccaaagaacc gatcaaaaac gaagccaaca acggcctgaa aaatacccgt ggtacgctgg
     6001 caatggcacg tactcaggct ccgcactctg caactgcaca gttcttcatc aacgtggttg
     6061 ataacgactt cctgaacttc tctggcgaaa gcctgcaagg ttggggctac tgcgtgtttg
     6121 ctgaagtggt tgacggcatg gacgtggtag acaaaatcaa aggtgttgca accggtcgta
     6181 acggtatgca ccaggacgtg ccaaaagaag acgttatcat tgaaagcgtg accgttagcg
     6241 agtaatcgtg gcgacactct ttattgcaga tcttcatctc tgcgtggaag aaccggcgat
     6301 caccgccggt tttctgcgtt ttttagcggg ggaagcccgc aaggccgacg cgctgtatat
     6361 tcttggcgat ctgtttgaag catggattgg cgacgacgat cccaacccac tccatcgcca
     6421 gatggcggca gcgatcaaag cggtgtccga ttccggcgtt ccctgttatt tcattcatgg
     6481 caaccgtgat tttctgctcg gcaaacgctt tgcccgtgaa agcggcatga cattattgcc
     6541 ggaagaaaag gtgctcgaac tttatggtcg ccgggtgttg attatgcatg gcgacacgct
     6601 gtgcaccgat gacgcgggtt atcaggcttt tcgcgccaaa gtccacaaac cctggctgca
     6661 aatgctattc ctcgccctgc cgttgtttgt gcgcaaacgc attgccgcgc gaatgcgcgc
     6721 gaacagcaaa gaagccaaca gcagtaaatc gctggcgatc atggacgtta accaaaacgc
     6781 tgtggtcagt gcgatggaaa aacatcaggt gcaatggcta atccacgggc atacccatcg
     6841 cccggcggtg catgaactta tcgccaacca gcaacctgct tttcgcgtgg tactgggtgc
     6901 ctggcatacg gaaggttcga tggtgaaagt cacggctgat gacgttgagc tgattcattt
     6961 tccgttttaa aaaacccaca actttgctga tttcacagcc acgcaaccgt tttccttgct
     7021 ctctttccgt gctattctct gtgccctcta aagccgagag ttgtgcacca caggagtttt
     7081 aagacgcatg tcttcccgca ataatccggc gcgtgtcgcc atcgtgatgg ggtccaaaag
     7141 cgactgggct accatgcagt tcgccgtcga aatcttcgaa atcctgaatg tcccgcacca
     7201 cgttgaagtg gtttctgctc accgcacccc cgataaactg ttcagcttcg ccgaaagcgc
     7261 cgaagagaac ggttatcagg tgattattgc gggcgcaggc ggcgcagcgc acctgccagg
     7321 catgattgcc gccaaaacgc tggtgccggt gctgggcgtg ccagtacaga gcgccgcact
     7381 gagcggtgtc gatagcctct actccatcgt acaaatgccg cgcggcattc cggtgggtac
     7441 gctggcgatt ggtaaagctg gcgcggcaaa cgcggcatta ctggcagcac aaattctcgc
     7501 gactcacgat aaagagctac accagcgtct gaatggctgg cgcaaagccc agaccgacga
     7561 agtgctggaa aacccggacc cgcgaggtgc ggcatgaaac aggtttgcgt cctcggtaac
     7621 gggcagttag gccgtatgct gcgtcaggca ggcgaaccgt taggcattgc tgtctggccg
     7681 gtcgggctgg acgctgaacc ggcggcggtg ccttttcaac aaagcgtgat taccgctgag
     7741 atcgaacgct ggccggaaac cgcattaacc cgcgagctgg cgcgtcatcc ggcctttgtg
     7801 aaccgcgatg tgttcccgat tattgctgac cgtctgactc agaaacagct tttcgataag
     7861 ctccacctgc cgaccgcgcc gtggcagtta cttgccgatc gcagcgagtg gcctgcggtg
     7921 tttgatcgtt taggtgaact ggcgattgtt aagcgtcgca ctggtggcta tgacggtcgc
     7981 ggtcaatgga gtttacgcgc caatgaaacc gaacagttac cggcagagtg ttacggtgaa
     8041 tgtattgtcg agcagggcat taacttctct ggtgaagtat cgctggttgg cgcgcgcggc
     8101 tttgatggca gcaccgtgtt ttatccgctg acgcataacc tgcatcagga cggtattttg
     8161 cgcaccagcg tcgcttttcc gcaggccaac gcacagcagc aggcgcaagc cgaagagatg
     8221 ctgtcggcga ttatgcagga gctgggctat gtgggcgtga tggcgatgga gtgttttgtc
     8281 accccgcaag gtctgctgat caacgaactg gctccgcgtg tgcataacag cggtcactgg
     8341 acacaaaacg gtgccagcat cagccagttt gagctgcatc tgcgggcgat taccgatctg
     8401 ccgttaccgc aaccagtagt gaataatccg tcggtgatga tcaatctgat tggtagcgat
     8461 gtgaattatg actggctgaa attgccgctg gtgcatctgc actggtacga caaagaagtc
     8521 cgtccggggc gtaaagtggg gcatctgaat ttgaccgaca gcgacacatc gcgtctgact
     8581 gcgacgctgg aagccttaat cccgctgctg ccgccagagt atgccagcgg cgtgatttgg
     8641 gcgcagagta agttcagtta agcaggtgct catttgattg ccggatgcgg cgtaaacgcc
     8701 ttatccggct taccgctccg gtacccattg taggcctgat aagacgcgtc aagcgtcgca
     8761 tcaggcatgg tgcaccgatt gccggatgct ctcatcacta cagcgaaata caggtccccg
     8821 cttcgcccgc cagcgtctct tcaattcgcg ataacgcccc aatccacgcg ggtttactac
     8881 ggcttctgac ataaccactc accgccgtta ccttcggccc catcgaacca tcggctttgg
     8941 caaatggcgc taactcatcc ggtgtggcat ggcgaatggc acgttgctgc ggcgttcccc
     9001 agttttcata taccgcatca gcatcggtga ggatcaccag tccatcggca ttaatctgct
     9061 cggcgagcaa cgcagcggcg agatctttat caatcaccgc ttcactccct gccccatctt
     9121 ccgtcacagg cacaccgccg ccgccactgc aaatcaccac atgcccctct ttgagcaaca
     9181 actcgatggc ttcgctgtcg agaattttac gcggttgcgg agacgccacc acccggcgca
     9241 aatatttacc atcacgtttc atctgccagc cgtaagccgc ttccagtgcc tcttgttctt
     9301 ctggctgata aaccggacca ataaatttct ctggctgcaa aaacgccgga tcatcaggcg
     9361 aaacctcaat gcgcgtcagc accgtcgtaa caggcggcat ctgcggctgt gcgctcaagc
     9421 tctgcgccag catatagcca atcatcccct ggctttccgc caccagtaca tccagcggat
     9481 acggttctac ctctttccac gccagattct gtaacgccag cagccccacc tgcggcccgt
     9541 tgccgtgaac aatcgccagc cgataagaac gggccaggcg tgccagcgcg ggtacagcac
     9601 tggcgatatt gcgatattga ttttccgccg tcagcgcctc accgcgctgg agtaaggcgt
     9661 tgcccccaag agctacaacc agtgttttca tggttttcct tgtaataatt gttggccaag
     9721 ccagaacccc agcagcgtat ctgcccctga cgtatgcccc agcgccagca gcgaatcaat
     9781 cgcaacagcg gtacgtttcg ggcaactcag agcatggaca aagtgcagga gtggcgaggc
     9841 gaaatatcct tgcgcggcat aacgtaaata actgacgctc accgctgtgg taacgagttg
     9901 aagattgtcg gaacaggcaa aaaacgggcg accggcgcgc gcatctaaag cgccataata
     9961 ccaggccgcc agcagcattc cgctcaacgt gtcatcatga cttggcgtta atccggggcc
    10021 tttacccagc cagtgccgcc agtcggtctt aacgccattg agcgcggcct gaaaacagtg
    10081 acgaaactgg cgtaattcag cgggcagcgg atcgcttgcc gccatcgcca gtggcccgaa
    10141 aagtccggtt tcctccgcgc gttgcatcca tgcagctgca agcggttgag gatgcgaagg
    10201 cggcgtaata cgcagcaaac aataacgctg tggctgttta accgtgaaac gcccgaggcg
    10261 aatcccttga gccacaacct gtggtcgttc attgccgcat aatccaccgc ataacgcatc
    10321 gaattgcgtg cggcgaaatt acacattccc gtcaagctat agaagaaacg ctgccctggc
    10381 tttcctcttt tggcgctgac ccaacgggtg ggatgacccg tttactttat tcgccggaat
    10441 ggctggaaac ccagcagcaa tttaaaaaaa gaatggcagc aagtgggctg gaaacacgtt
    10501 tcgatgaagt ggggaattta tacggtcgcc tgagtggcac cgaatatcca caggaagtgg
    10561 ttctgagcgg ttcgcatatc gataccgtgg ttaacggcgg taacctcgac gggcaattcg
    10621 gcgcgctggc ggcgtggctg gcaattgact ggttgaaaac gcaatacggc gcaccgttac
    10681 gtacggtcga agtggtggcg atggcagaag aagaaggcag ccgcttcccg tatgtcttct
    10741 ggggcagtaa aaatatcttt gggctggcga atcctgacga cgtgcggaat atctgtgatg
    10801 ccaaaggaaa tagttttgcc gatgcgatga aggcttgcgg atttactctg ccgaacgccc
    10861 cactaactcc gcgtcaggct ataaaagcct ttgttgaact gcatattgaa cagggctgtg
    10921 tgctggaaag taatgggcaa tcaattggcg tggtgaatgc aattgtcggg cagcgtcgct
    10981 atacggtgac gctgaacggc gaatcaaacc atgcaggcac cacgccgatg ggttatcgtc
    11041 gtgatacggt ttacgctttc agtcgcattt gccatcagtc gatcgaaaaa gcgaaaaaga
    11101 tgggcgatcc gctggttctg acctttggca aagtagagcc gcgcccgaat acggtaaatg
    11161 tggtgccggg taaaaccacg ttcaccattg attgtcgtca taccgacgct gccgtgctgc
    11221 gcgatttcac ccaacagtta gaaaacgaca tgcgggcgat ttgcgatgaa atggacattg
    11281 gtattgatat cgatttatgg atggacgaag aacccgtgcc gatgaataag gagctggtcg
    11341 ccaccctgac agaattgtgt gaaagagaaa aactgaatta ccgggtgatg cacagtggtg
    11401 ccgggcacga cgcgcaaatt ttcgcgcctc gcgtaccaac ctgcatgatt tttatcccca
    11461 gcatcaacgg gatcagccat aacccggcgg aacgcaccaa tattactgac cttgccgaag
    11521 gggtcaaaac gttggcactc atgctttatc aacttgcctg gcaggaataa ggagtcataa
    11581 atgggatatt taaataacgt caccggttac cgcgaagatt tactggctaa ccgtgcgatt
    11641 gttaaacacg gtaatttcgc actgttaacc ccggatggtc tgataaaaaa tattattccg
    11701 gactttgaaa attgtgacgc gacaatcctc tccacgccaa agctgggtgc ctcttttgtt
    11761 gattatctgg tcacactgca tcaaaacggt ggcaaccaac agggcttcgg tggcgaaggc
    11821 attgaaacgt tcctgtatgt gatctctgga aatatcactg ccaaagccga aggcaaaaca
    11881 tttgccttaa gcgaaggtgg ctatctttat tgcccaccag gctccttaat gacgtttgtt
    11941 aacgcccagg ccgaagacag ccaaatcttt ttatataagc gccgctatgt tccggtagaa
    12001 ggccatgcac cgtggctgtt ttctggcaat gccagcgaac tggaacgcat tcactatgaa
    12061 ggtatggatg atgtgatcct gctggatttt ctgcccaaag agttaggttt tgatatgaac
    12121 atgcatatcc tctcttttgc accaggtgcc agccacggtt atatcgaaac acacgttcag
    12181 gaacatggtg cctatattct ttccggtcag ggggtttata
//
LOCUS       DAJTRC010000106        12102 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.106, whole genome shotgun sequence.
ACCESSION   DAJTRC010000106 DAJTRC010000000
VERSION     DAJTRC010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 12102)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12102)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12102
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.106"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..359
                     /locus_tag="OO357_003282"
     CDS             <1..359
                     /locus_tag="OO357_003282"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888122.1"
                     /translation="SWDAVLYDLRSEKIISKDNYLTDLNRRHLELVLHTNPNPSGSVT
                     KILRTIFTKQVKSLGPELCHFLHHELTKKRYEVWHHYDVLHYQSSSFSRSQFNALINL
                     LAQQSRFADLKLSNYK"
     gene            complement(447..1091)
                     /gene="cheZ"
                     /locus_tag="OO357_003283"
     CDS             complement(447..1091)
                     /gene="cheZ"
                     /locus_tag="OO357_003283"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase CheZ"
                     /protein_id="HCR5888123.1"
                     /translation="MMQPSIKPADEHSAGDIIARIGSLTHMLRDSLRELGLVQAIAEA
                     AEAIPDARDRLYYVVQMTAQAAERALNSVEASQPHQDQMEKSAKALTQRWDDWFADPI
                     DLADARELVTDTRQFLADVPAHTSFTNAQLLEIMMAQDFQDLTGQVIKRMMDVIQEIE
                     RQLLMVLLENIPEQESRPKRENQSLLNGPQVDTSKAGVVASQDQVDDLLDSLGF"
     gene            complement(1102..1491)
                     /gene="cheY"
                     /locus_tag="OO357_003284"
     CDS             complement(1102..1491)
                     /gene="cheY"
                     /locus_tag="OO357_003284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002210873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator CheY"
                     /protein_id="HCR5888124.1"
                     /translation="MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALN
                     KLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAG
                     ASGYVVKPFTAATLEEKLNKIFEKLGM"
     gene            complement(1506..2555)
                     /gene="cheB"
                     /locus_tag="OO357_003285"
     CDS             complement(1506..2555)
                     /gene="cheB"
                     /locus_tag="OO357_003285"
                     /EC_number="3.1.1.61"
                     /EC_number="3.5.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000036372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate methylesterase/protein
                     glutamine deamidase"
                     /protein_id="HCR5888125.1"
                     /translation="MSKIRVLSVDDSALMRQIMTEIINSHSDMEMVATAPDPLVARDL
                     IKKFNPDVLTLDVEMPRMDGLDFLEKLMRLRPMPVVMVSSLTGKGSEVTLRALELGAI
                     DFVTKPQLGIREGMLAYSEMIAEKVRTAAKASLAAHKPLSAPTTLKAGPLLSSEKLIA
                     IGASTGGTEAIRHVLQPLPLSSPALLITQHMPPGFTRSFADRLNKLCQIGVKEAEDGE
                     RVLPGHAYIAPGDRHMELARSGANYQIKIHDGPAVNRHRPSVDVLFHSVAKQAGRNAV
                     GVILTGMGNDGAAGMLAMRQAGAWTLAQNEASCVVFGMPREAINMGGVCEVVDLSQVS
                     QQMLATISAGQAIRI"
     gene            complement(2558..3418)
                     /gene="cheR"
                     /locus_tag="OO357_003286"
     CDS             complement(2558..3418)
                     /gene="cheR"
                     /locus_tag="OO357_003286"
                     /EC_number="2.1.1.80"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HCR5888126.1"
                     /translation="MTSSLPCGQTSLLLQMTERLALSDAHFRRISQLIYQRAGIVLAD
                     HKRDMVYNRLVRRLRSLGLTDFGHYLNLLESNQHSGEWQAFINSLTTNLTAFFREAHH
                     FPLLADHARRRSGEDRVWSAAASTGEEPYSIAMTLADTLGTVPGRWKVFASDIDTEVL
                     EKARSGIYRHEELKNLTPQQLQRYFMRGTGPHEGLVRVRQELANYVDFAPLNLLAKQY
                     TVPGPFDAIFCRNVMIYFDQTTQQEILRRFVPLLKPDGLLFAGHSENFSHLERRFTLR
                     GQTVYALSKD"
     gene            complement(3437..5038)
                     /gene="tap"
                     /locus_tag="OO357_003287"
     CDS             complement(3437..5038)
                     /gene="tap"
                     /locus_tag="OO357_003287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein IV"
                     /protein_id="HCR5888127.1"
                     /translation="MFNRIRISTTLFLILILCGILQIGSNGMSFWAFRDDLQRLNHGE
                     QSNQQRAALAQTRAVMLQASTALNKAGTLTALSYPADDIKTLMTTARASLTQSTTLFK
                     SFMAMTAGNEHVRALQKETEKSFARWHNDLEHQATWLESNQLSDFLTAPVQGSQNAFD
                     VNFEAWQLEINHVLEAASAQSQRNYQISALVFISMIIVAAIYISSALWWTRKMIVQPL
                     AIIGSHFDSIAAGNLARPIAVYGRNEITAIFASLKTMQQALRGTVSDVRKGSHEMHIG
                     IAEIVAGNNDLSSRTEQQAASLAQTAASMEQLTATVGQNADNARQASELAKNAATTAQ
                     AGGVQVSTMTHTMQEIATSSQKIGDIISVIDGIAFQTNILALNAAVEAARAGEQGRGF
                     AVVAGEVRNLASRSAQAAKEIKGLIEESVNRVQQGSKLVNNAAATMTDIVSSVTRVND
                     IMGEIASASEEQRRGIEQVAQAVSQMDQVTQQNSSLVEEAAVATEQLANQADHLSSRV
                     AVFTLEEHEVARHESAQLQIAPVVS"
     gene            complement(5084..6745)
                     /gene="tar"
                     /locus_tag="OO357_003288"
     CDS             complement(5084..6745)
                     /gene="tar"
                     /locus_tag="OO357_003288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein II"
                     /protein_id="HCR5888128.1"
                     /translation="MINRIRVVTLLVMVLGVFALLQLISGSLFFSSLHHSQKSFVVSN
                     QLREQQGELTSTWDLMLQTRINLSRSAVRMMMDSSNQQSNAKVELLDSARKTLAQAAT
                     HYKKFKSMAPLPEMVATSRNFDEKYKNYYTALTELIDYLDYGNTGAYFAQPTQGMQNA
                     MGEAFAQYALSSEKLYRDIVTDNADDYRFAQWQLAVIALVVVLILLVAWYGIRRMLLT
                     PLAKIIAHICEIAGGNLANTLTIDGRSEMGDLAQSVSHMQRSLTDTVTHVREGSDAIY
                     AGTREIAAGNTDLSSRTEQQASALEETAASMEQLTATVKQNADNARQASQLAQSASDT
                     AQHGGKVVDGVVKTMHEIADSSKKIADIISVIDGIAFQTNILALNAAVEAARAGEQGR
                     GFAVVAGEVRNLASRSALAAKEIKALIEDSVSRIDTGSVLVESAGETMNNIVNAVTRV
                     TDIMGEIASASDEQSRGIDQVALAVSEMDRVTQQNASLVQESAAAAAALEEQASRLTQ
                     AVSAFRLAASPLTNKPQTPSRPASEQPPAQPRLRITEQDPNWETF"
     gene            complement(6890..7393)
                     /gene="cheW"
                     /locus_tag="OO357_003289"
     CDS             complement(6890..7393)
                     /gene="cheW"
                     /locus_tag="OO357_003289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HCR5888129.1"
                     /translation="MTGMTNVTKLASEPSGQEFLVFTLGDEEYGIDILKVQEIRGYDQ
                     VTRIANTPAFIKGVTNLRGVIVPIVDLRIKFSQVDVDYNDNTVVIVLNLGQRVVGIVV
                     DGVSDVLSLTAEQIRPAPEFAVTLSTEYLTGLGALGDRMLILVNIEKLLNSEEMALLD
                     SAASEVA"
     gene            complement(7414..9378)
                     /gene="cheA"
                     /locus_tag="OO357_003290"
     CDS             complement(7414..9378)
                     /gene="cheA"
                     /locus_tag="OO357_003290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HCR5888130.1"
                     /translation="MSIDISDFYQTFFDEADELLADMEQHLLVLQPEAPDAEQLNAIF
                     RAAHSIKGGAGTFGFSVLQETTHLMENLLDEARRGEMQLNTDIINLFLETKDIMQEQL
                     DAYKQSQEPDAASYDYICQALRQLALEAKGETPSAVTRLSVVAKSEPQDEQSRSQLPR
                     RIILSRLKAGEVDLLEEELGHLTTLNDVVKGADSLSAILPGDIAEDDITAVLCFVIEA
                     DQITFETVEVSPKIATPPVLKLAAEQAPTGRVEREKTTRSSESTSIRVAVEKVDQLIN
                     LVGELVITQSMLAQRSSELDPVNHGDLITSMGQLQRNARDLQESVMSIRMMPMEYVFS
                     RYPRLVRDLAGKLGKQVELTLVGSSTELDKSLIERIIDPLTHLVRNSLDHGIELPEKR
                     LAAGKNSVGNLILSAEHQGGNICIEVTDDGAGLNRERILAKAASQGLTVSENMSDDEV
                     AMLIFAPGFSTAEQVTDVSGRGVGMDVVKRNIQEMGGHVEIQSKQGTGTTIRILLPLT
                     LAILDGMSVRVADEVFILPLNAVMESLQPREADLHPLAGGERVLEVRGEYLPIVELWK
                     VFNVAGAKTEATQGIVVILQSGGRRYALLVDQLIGQHQVVVKNLESNYRKVPGISAAT
                     ILGDGSVALIVDVSALQAINREQRMANTAA"
     gene            complement(9383..10309)
                     /gene="motB"
                     /locus_tag="OO357_003291"
     CDS             complement(9383..10309)
                     /gene="motB"
                     /locus_tag="OO357_003291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotB"
                     /protein_id="HCR5888131.1"
                     /translation="MKNQAHPIIVVKRRKAKSHGAAHGSWKIAYADFMTAMMAFFLVM
                     WLISISSPKELIQIAEYFRTPLATAVTGGDRISNSESPIPGGGDDYTQSQGEVNKQPN
                     IEELKKRMEQSRLRKLRGDLDQLIESDPKLRALRLHLKIDLVQEGLRIQIIDSQNRPM
                     FRTGSADVEPYMRDILRAIAPVLNGIPNRISLSGHTDDFPYASGEKGYSNWELSADRA
                     NASRRELMVGGLDSGKVLRVVGMAATMRLSDRGPDDAVNRRISLLVLNKQAEQAILHE
                     NAESQNEPVSALEKPEVAPQVSVPTMPSAEPR"
     gene            complement(10306..11193)
                     /gene="motA"
                     /locus_tag="OO357_003292"
     CDS             complement(10306..11193)
                     /gene="motA"
                     /locus_tag="OO357_003292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HCR5888132.1"
                     /translation="MLILLGYLVVLGTVFGGYLMTGGSLGALYQPAELVIIAGAGIGS
                     FIVGNNGKAIKGTLKALPLLFRRSKYTKAMYMDLLALLYRLMAKSRQMGMFSLERDIE
                     NPRESEIFASYPRILADSVMLDFIVDYLRLIISGHMNTFEIEALMDEEIETHESEAEV
                     PANSLALVGDSLPAFGIVAAVMGGVHALGSADRPAAELGALIAHAMVGTFLGILLAYG
                     FISPLASVLRQKSAETSKMMQCVKVTLLSNLNGYAPPIAVEFGRKTLYSSERPSFIEL
                     EEHVRAVKNPQQQTTTEEA"
     gene            complement(11320..11898)
                     /gene="flhC"
                     /locus_tag="OO357_003293"
     CDS             complement(11320..11898)
                     /gene="flhC"
                     /locus_tag="OO357_003293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhC"
                     /protein_id="HCR5888133.1"
                     /translation="MSEKSIVQEARDIQLAMELITLGARLQMLESETQLSRGRLIKLY
                     KELRGSPPPKGMLPFSTDWFMTWEQNVHASMFCNAWQFLLKTGLCNGVDAVIKAYRLY
                     LEQCPQAEEGPLLALTRAWTLVRFVESGLLQLSSCNCCGGNFITHAHQPVGSFACSLC
                     QPPSRAVKRRKLSQNPADIIPQLLDEQRVQAV"
     gene            complement(11901..>12102)
                     /locus_tag="OO357_003294"
     CDS             complement(11901..>12102)
                     /locus_tag="OO357_003294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416406.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar transcriptional regulator FlhD"
                     /protein_id="HCR5888134.1"
                     /translation="LPQMVKLAETNQLVCHFRFDSHQTITQLTQDSRVDDLQQIHTGI
                     MLSTRLLNDVNQPEEALRKKRA"
BASE COUNT         2780 a         3384 c         2932 g         3006 t
ORIGIN      
        1 tgagttggga tgccgtgctc tatgatctac gtagtgaaaa aattatcagt aaagataatt
       61 acttgaccga cttaaaccgc agacatcttg aacttgttct tcatactaac cccaatccaa
      121 gcggttcggt aacgaagatt ttacgcacta tttttactaa gcaggtaaag agtttagggc
      181 cggaactctg tcatttcttg caccatgaac tgacgaaaaa acgttatgaa gtatggcatc
      241 actacgatgt actgcactac cagtcatcgt cattttctcg ttcacaattt aatgcattga
      301 taaatttact ggcgcaacaa tcacgcttcg ctgatctaaa attatcaaac tacaaataac
      361 catcctccac gtactccctg aacgataccc gccaccctgc tatcggcttg gcatcatcgc
      421 gcaggccgaa gttcaggcaa tacaaatcaa aatccaagac tatccaacaa atcgtccacc
      481 tgatcctgac tggctaccac gccggctttg ctggtatcga cctgaggtcc attaagcaaa
      541 ctctggtttt cacgttttgg acgcgactcc tgttccggga tgttttccaa cagcaccatc
      601 agcaactggc gttcgatctc ctgaatgaca tccatcatcc gcttaataac ctgcccggtg
      661 agatcctgaa aatcctgcgc catcatgatt tccagcagtt gcgcgttagt aaagctggta
      721 tgcgcgggta catctgccag aaattgtcgt gtatctgtta ccagttcacg ggcgtcggca
      781 aggtcaatcg gatcggcaaa ccagtcatcc caacgttggg ttaacgcttt tgctgatttc
      841 tccatttgat cctgatgcgg ttgtgacgcc tcaacactgt tcagcgcccg ctccgcagcc
      901 tgggcggtca tctgcacaac atagtacaaa cgatcgcgcg catcggggat ggcttccgcc
      961 gcttcggcaa tggcctgaac cagccccagt tcccgcaaac tgtcgcgcag catatgcgtc
     1021 aggctgccga tgcgcgcaat gatatcgcca gctgaatgct cgtcagcagg tttgattgat
     1081 ggttgcatca tagtcgcatc ctcacatgcc cagtttctca aagattttgt tgagtttttc
     1141 ctccagcgtc gcggcggtaa atggcttcac cacatagcca ctggcccccg cttgcgccgc
     1201 agcaatgatg ttctctttct tcgcttctgc agtcaccatt aacactggca atgccgacat
     1261 cgcgccatcc gcacgaattg ttttcagcaa ttccaggcca tccatattgg gcatgttcca
     1321 gtcggagata acaaatccat aaccgcctgc ctgcaactta ttgagagcgt cgacaccatc
     1381 ttccgcttcc tcaacattat tgaatcccag ctctttcagc aggttacgca ctatgcgtcg
     1441 catggtggaa aagtcatcca caaccaaaaa tttaagttct ttatccgcca tttcacactc
     1501 ctgatttaaa tacgtatcgc ctgtccggca ctaattgttg ccaacatttg ctggcttacc
     1561 tggctaagat cgaccacttc gcagacacca cccatattga tggcctcgcg cggcatgccg
     1621 aacaccacgc aacttgcttc gttttgcgca agggtccatg cccccgcctg acgcatcgcc
     1681 aacattcccg ccgcgccgtc gttgcccata ccggtcagga tcaccccaac cgcattacgc
     1741 cccgcctgtt tggcgacaga atggaacaac acatctaccg aaggccgatg acggttaacc
     1801 gccgggccgt cgtgaatttt gatttgataa tttgcgccac tacgcgccag ttccatatgc
     1861 cgatcgcccg gcgcaatata ggcatgcccc ggcaagacac gttctccgtc ttcggcttct
     1921 ttaaccccga tctggcaaag cttattaagt ctgtcggcaa aagagcgggt gaaaccgggc
     1981 ggcatatgtt gggtaattaa caatgccggg ctggaaagcg gcaacggttg cagtacgtga
     2041 cgaattgcct cagttccacc cgttgaagca ccaatcgcaa tcagtttttc agaactcaac
     2101 aacggccctg ccttcagcgt tgtcggtgct gacaatggct tatgtgctgc aaggctcgcc
     2161 tttgctgccg tacgcacctt ttcagcaatc atttcgctat acgccagcat tccttcgcga
     2221 atacccagtt gcggtttggt gacaaaatct attgccccca gctccagcgc gcgcagcgtg
     2281 acttctgatc ctttgccggt caaggaagaa accatcacaa cgggcattgg acgcaaacgc
     2341 attaattttt cgaggaaatc cagtccgtcc atccgcggca tttcaacatc cagcgtcagc
     2401 acatcgggat tgaatttctt aatcaagtca cgcgcaacca gcggatcagg cgcggtcgcc
     2461 accatttcca tgtcgctatg gctgttgatg atttctgtca tgatctggcg catcagtgcc
     2521 gaatcatcga cagataacac cctgattttg ctcatcgtta atccttactt agcgcataca
     2581 ccgtctgacc acgcagcgtg aagcggcgct cgaggtggct aaagttttca gagtgacccg
     2641 caaacagcaa tccgtcgggt ttaaggagcg gaacaaagcg gcgcaaaatc tcctgctggg
     2701 tagtttgatc gaagtagatc atgacgttac gacagaagat cgcatcaaac ggccccggca
     2761 cggtgtactg tttcgccagt aaattcagcg gggcaaaatc aacatagttc gccagctcct
     2821 gacgcacgcg taccagccct tcatgcggtc ccgtccctcg catgaaatac cgttgcagtt
     2881 gctgcggcgt caggtttttc aactcttcat ggcgatagat accgcttctg gctttttcca
     2941 gcacttcggt gtcgatatca ctggcaaaca ctttccagcg tccgggcacg gtgcccaatg
     3001 tgtcagccag agtcatcgca atgctgtacg gctcttcgcc ggtcgaagcc gccgcgctcc
     3061 atacgcgatc ctcgccagaa cggcgacgtg cgtgatccgc gagcagaggg aaatgatgag
     3121 cctcacggaa aaatgccgtc agattcgtgg tcagcgaatt gataaacgcc tgccactcac
     3181 cgctgtgctg attagattcc agcaagttca gataatgacc gaaatccgtc agtcccagag
     3241 aacgcaaacg acgaaccagt cggttgtaaa ccatgtcgcg tttatggtca gccagaacga
     3301 tcccggctcg ttgatagatc aattgactta tccgccgaaa atgcgcgtcg gaaagcgcca
     3361 ggcgctcggt catctgtaac aataaagacg tttgcccaca gggcagagat gaagtcatag
     3421 cgccttctca atcacttcag gataccactg gcgcaatttg taactgcgcc gactcatgtc
     3481 gtgctacttc atgttcttca agggtaaata ccgccacacg cgacgaaaga tggtcggcct
     3541 gattcgccag ttgttccgtt gccaccgccg cttcttctac cagcgaggag ttctgctgag
     3601 tcacctgatc catctggctg acagcctgtg caacctgctc aatcccccgc cgttgttctt
     3661 ccgacgccga agcaatttct cccataatgt cgttcacgcg ggtcaccgaa ctgacaatat
     3721 cggtcatggt cgcggcggcg ttattcacca gtttcgaacc ctgctggaca cgattgactg
     3781 actcttcgat cagccctttg atctcttttg ctgcctgcgc gctacggctg gcaagattgc
     3841 gcacttcacc tgccactacc gcaaaaccac gcccctgctc tccggcgcga gccgcttcca
     3901 ctgccgcatt cagggccaga atattggtct ggaaagcaat tccgtcgata acgctgataa
     3961 tgtcgccaat tttttgcgag ctggtggcga tctcctgcat ggtgtgagtc atggtactga
     4021 cctgaacacc gcccgcctgc gccgttgtcg cggcattttt tgccagttcc gacgcctgtc
     4081 gtgcgttatc ggcgttttgc cctaccgtgg cggttaattg ctccatactg gcggccgttt
     4141 gtgccagcga tgccgcctgc tgttcggtac gacttgagag atcgttattg cctgcgacaa
     4201 tctccgcgat cccaatgtgc atctcatggc ttcccttacg cacatcactc accgtcccac
     4261 gcaaagcctg ctgcatggtc ttcaggctgg caaaaatggc ggtgatctca ttacgaccat
     4321 ataccgcaat cggacgcgcc agattacccg cagcaatgct gtcaaaatgg ctaccgataa
     4381 tggccagtgg ttgaacaatc atcttgcgcg tccaccacag cgcactgctg atgtagatcg
     4441 ccgcaacaat aatcatgctg ataaacacca gcgccgaaat ctgatagtta cgctggcttt
     4501 gcgcactggc ggcttccagc acatggttga tctccagctg ccaggcctca aagttaacgt
     4561 caaacgcatt ctgtgatccc tgcaccggcg cagtgaggaa atccgaaagt tgattacttt
     4621 caagccaggt cgcctgatgt tcgagatcgt tgtgccagcg ggcaaaactt ttctccgtct
     4681 ctttttgcaa tgccctgacg tgctcgttgc ccgcagtcat cgccataaaa cttttaaaca
     4741 gcgtggtgga ttgcgtcaga ctggcgcgcg ccgtcgtcat caacgtttta atgtcatccg
     4801 ccggatagct aagcgctgtc agagtgcctg ctttgttcag cgcggtactg gcctgtaaca
     4861 ttaccgcccg agtttgcgct aatgccgcac gttgctgatt gctctgctcg ccgtgattca
     4921 gtcgttgcaa atcgtcgcga aatgcccaaa aagacatgcc gttactgcca atctgcaaga
     4981 tcccgcagag aatcaaaatt aaaaacagcg tggtcgaaat tcgaatacga ttaaacatca
     5041 acgctcccca tcaggcggca atgaccgcgt tagtaaaaac tcgtcaaaat gtttcccagt
     5101 ttggatcttg ttcagtaatt cgcagtcgtg gctgtgccgg tggttgctca ctggcaggac
     5161 gggatggtgt ttgcggttta ttggtgagtg ggctggctgc cagacggaac gcggaaaccg
     5221 cttgcgttaa acgactcgcc tgttcttcca gcgcagctgc ggcggcagct gattcctgca
     5281 ccagcgatgc gttctgttgc gtgacgcgat ccatttccga aaccgccaat gcgacttgat
     5341 cgatgccacg gctctgttca tccgatgccg atgcaatctc gcccataatg tcagtcacgc
     5401 gagtgacagc attgacgata ttgttcattg tttccccggc gctttcgacc agcaccgaac
     5461 cggtatcaat gcgcgagacg gagtcttcaa tgagggcttt gatctctttt gccgccaggg
     5521 cgctgcgact ggcaagatta cgcacttcac ccgccaccac ggcaaaacca cggccctgtt
     5581 cacccgcacg cgcggcttca accgcggcat tcagcgcgag gatattagtc tggaaggcaa
     5641 taccgtcgat aacgctgata atgtcggcaa ttttcttcga actatcggcg atctcatgca
     5701 tcgttttcac tacgccatcc accactttgc cgccgtgctg ggcggtgtcg gaggcacttt
     5761 gcgccagttg cgaggcctgg cgggcgttat cggcgttttg cttcactgtc gcggtgagct
     5821 gctccatgct ggcggcagtt tcttccagcg cggatgcctg ctgttcagta cgggaggaaa
     5881 gatcggtgtt gcccgccgca atttcacggg taccggcata gatggcatct gaaccttcgc
     5941 ggacatgagt gacggtgtca gtcaaagagc gttgcatatg tgaaacgctc tgcgccaggt
     6001 cacccatttc actgcgcccg tcaatggtca gggtattcgc caggttacca ccggcgattt
     6061 cgcaaatgtg agcaataatt tttgccagcg gagtaagcaa catacggcga atgccgtacc
     6121 acgccaccag cagaatcaat accaccacca gcgcgataac cgccagttgc cactgggcaa
     6181 atcggtaatc atctgcgttg tcagtgacga tatcgcgata cagtttttca ctgctgaggg
     6241 cgtactgagc aaacgcttcg cccattgcat tttgcattcc ctgggttggc tgagcgaaat
     6301 aagctccagt attgccataa tcaagataat caatcagttc agttaacgct gtgtaatagt
     6361 ttttatattt ttcatcaaaa ttacggctgg tagcgaccat ttcaggtaac ggtgccatgc
     6421 ttttgaattt tttataatgc gtcgcggcct gggccaatgt tttcctggcg ctatcgagca
     6481 attcaacttt ggcgttactt tgttgattgg aggaatccat catcatccgc accgctgaac
     6541 gactcaggtt aatgcgcgtt tgcagcatta aatcccaggt tgacgtcagc tcgccctgct
     6601 gttcccgtaa ttgattggaa accacaaagc tcttctggct atggtgaagg gaagaaaaaa
     6661 acaaactgcc ggaaataagc tgtaacagtg cgaatacccc cagcaccatt accaacagcg
     6721 tgactacgcg gatacggtta atcataaggc accttcctga aaacaagttg atctcgttat
     6781 cggcaagaag gggggaaact ttattgctga tgccacccgc cgcgaaattg aaataaaaaa
     6841 cccgatgcgc agatcatcgg gttcatttca attgaggaaa tcgggagaat tacgccactt
     6901 ctgacgccgc gctatctaac agcgccatct cttcgctgtt cagcagtttt tcgatgttca
     6961 ccagaatcaa catacggtcg cccagtgcgc ccagtccagt gagatattct gttgaaagcg
     7021 tcacggcaaa ctctggtgcc ggacgaattt gctccgccgt caatgaaagc acgtctgaaa
     7081 cgccgtcaac cacgatgccg accacccgct gtccgagatt caggacgata actaccgtgt
     7141 tgtcgttata gtccacatcc acctggctga acttaattcg taagtcaaca atcggcacaa
     7201 taacgccgcg cagattcgtg acgcctttga taaacgctgg cgtgttcgca atccgtgtta
     7261 cctgatcgta gccacggatc tcctgcactt tcaggatatc aataccgtac tcttcatcac
     7321 caagggtaaa taccagaaat tcctggcctg acggctcgct ggccagcttt gttacattcg
     7381 tcataccggt catattgtta cctttttact cattcaggcg gcggtgttcg ccatacgttg
     7441 ttcgcggttt atcgcctgca aggcggagac atcaacaatc agtgccacgc tgccgtcgcc
     7501 aagaatggtc gcagcagaaa tgccggggac tttgcgatag ttactttcaa ggtttttaac
     7561 cacaacctgg tgttgaccaa ttaattgatc caccagcaag gcgtagcggc gaccgccact
     7621 ttgtaagatc accacaattc cctgggtggc ttcggttttc gcgcccgcga cgttgaacac
     7681 tttccacagt tcgacgatgg gcagatattc accccgcact tccagcaccc gctcgccgcc
     7741 ggccagtgga tggagatcgg cttcacgggg ttgcagtgat tccataacag cattcagcgg
     7801 cagaatgaaa acttcatccg caacgcgtac ggacatgccg tcgaggatgg ccagcgtcag
     7861 cggcagtaaa atgcggatcg tagtgccagt accctgcttc gactggattt cgacatgacc
     7921 gcccatctcc tggatattac gtttaacgac gtccatgccg acaccgcgcc cggagacgtc
     7981 ggtgacctgc tctgccgtgg agaagcctgg tgcaaatatc agcattgcaa cttcgtcgtc
     8041 gctcatgttt tcgctgacag tcaaaccttg cgaggccgct tttgccagaa ttcgctcacg
     8101 gtttagcccc gccccatcgt cggtcacttc aatgcaaatg ttgccgccct gatgttcggc
     8161 agacagaatt aaatttccga cgctgttttt acctgcggcg agccgttttt ctggcagttc
     8221 aataccgtga tcgaggctat tgcgtaccag gtgggtcagc gggtcgataa tgcgttctat
     8281 caggctcttg tcgagctcgg tggaactgcc caccagcgtc agttctacct gcttgccgag
     8341 ttttcccgcc agatcacgca ccagccgggg atagcgacta aagacatatt ccatcggcat
     8401 catgcgaatc gacatcactg attcctgcaa atcacgggcg ttacgttgta actgccccat
     8461 gctggtaatc aaatcaccat gattaaccgg gtccagttcg ctggaacgct gggcaagcat
     8521 ggactgggtg ataaccagct cgccgacgag gttaattaat tgatcaacct tttctaccgc
     8581 tacacggatg ctggtggatt cactgctgcg cgtcgttttt tcccgctcca cgcggccggt
     8641 tggcgcttgt tcggctgcca gtttaagcac aggtggggta gctatttttg gcgaaacttc
     8701 tactgtttca aaggtaatct gatcggcttc aatcacaaaa cagagtaccg ctgtgatgtc
     8761 atcttcggcg atatcgcccg gtaatattgc cgagagagaa tccgcccctt tcaccacgtc
     8821 atttaacgtt gtcagatgcc ccagctcttc ttccagcagg tcgacttcgc cggccttcag
     8881 gcgcgaaagg ataattcgtc gcggcaactg actgcgactt tgctcatctt gcggttcact
     8941 tttggcaacc acacttaatc gggtcactgc ggatggcgtt tcgcctttcg cttctaatgc
     9001 cagttgacgc aaggcctggc agatataatc gtagctggcg gcatccggct cttgcgactg
     9061 tttataagcg tcgagctgtt cttgcatgat gtccttcgtt tccaaaaaca gattgataat
     9121 gtcggtgttg agttgcatct cacctcgtct ggcttcatcg agcaggtttt ccatcagatg
     9181 cgtggtttcc tgcaaaacgc tgaagccaaa agttcctgcc cctcctttga tcgagtgggc
     9241 agcccgaaag atggcattca attgttcggc atctggcgct tccggctgca aaaccagcag
     9301 atgctgctcc atatcagcca acagttcgtc cgcttcatca aaaaatgtct gataaaaatc
     9361 gcttatatcg atgctcacgc tgtcacctcg gttcggctga tggcattgtg ggaacactga
     9421 cctgtggtgc aacctcaggt ttttctaggg cgcttactgg ctcattctgg ctttcggcgt
     9481 tttcatgcaa aatggcctgt tcggcttgtt tgttcagtac caacaggctg atgcgacggt
     9541 tgacggcatc atcaggtccg cgatcgctta agcgcatcgt tgccgccatg ccgacgacac
     9601 gtaacacttt gccgctatcc aaccctccga ccatcagttc gcggcgggat gcattggccc
     9661 gatcggcaga aagctcccag ttgctgtaac ctttctcacc gctggcgtag ggaaaatcat
     9721 cggtatgacc tgaaaggcta atacggttgg gaataccgtt cagcacaggc gcgatggcgc
     9781 gcagaatgtc gcgcatatag ggttcgacat cggcactgcc ggttctaaac atcgggcgat
     9841 tctggctatc gatgatctga atacgtagac cttcctggac cagatcgatt ttgagatgga
     9901 gacgtaacgc ccgcagtttc ggatcggact ctatcaactg gtcgagatca ccccgcaatt
     9961 tccgcaatcg actttgctcc atgcgttttt tcagctcttc gatgttcggc tgcttattca
    10021 cttccccctg gctttgggtg taatcatcac caccgccggg aattgggctt tcactattgg
    10081 aaatgcgatc gccgcccgta accgctgtcg ccagtggagt ccggaagtac tccgcaatct
    10141 gaatcaactc ttttgggctg gagatggaga tcagccacat caccagaaaa aaggccatca
    10201 tcgcagtcat aaagtcggca taagcaatct tccacgatcc atgtgctgcc ccgtggcttt
    10261 tggctttgcg tcgtttgacg acaataatcg gatgcgcttg attcttcatg cttcctcggt
    10321 tgtcgtctgt tgttgcggat ttttcaccgc acgcacatgc tcttccagtt caataaacga
    10381 cggacgttcg ctggaataga gcgttttgcg accaaactca acggcgatag gcggtgcgta
    10441 accgttcaga ttagaaagca gagtgacttt gacgcactgc atcattttgc tggtttcggc
    10501 gcttttctga cgtaaaacac tcgctagtgg ggaaataaat ccgtaagcca ataaaatacc
    10561 gaggaaagtc cccaccatcg catgtgcgat aagcgccccc agctcggcgg caggacgatc
    10621 ggctgaacct aacgcgtgaa cgccccccat tacagccgcg acaataccaa acgccggaag
    10681 tgagtccccg accagcgcca gactgttcgc cgggacttct gcctcgcttt cgtgcgtctc
    10741 aatctcttca tccatcaggg cttcgatttc gaaggtgttc atgtgaccgc tgataatcag
    10801 gcgcagataa tcgacgataa aatcaagcat gacgctatcc gcgaggatgc gtgggtagct
    10861 ggcgaagatc tcgctctcac ggggattttc aatatcacgt tccagcgaaa acatccccat
    10921 ctgccgcgat ttcgccatca accgataaag cagagccagc agatccatat acattgcttt
    10981 ggtgtatttg gagcgacgaa acagcaacgg cagcgctttc agcgtgcctt taatcgcttt
    11041 gccattattg ccgacgataa acgacccaat ccctgcaccg gcaataatca ccagttcagc
    11101 gggttgatag agtgctccaa ggcttccacc ggtcatcaaa taaccgccga aaactgtacc
    11161 gagaacaacc aggtaaccta ataagataag cacgacatca tccttccact gttgaccatg
    11221 acaggatgtt cagtcgtcag gcgttaacgc gcgattgggg aaaaaaaaag cagcggtacg
    11281 tcgttaccgc tgctggaatg ttgcgccaca ccgtatcagt taaacagcct gtactctctg
    11341 ttcatccagc agttgtggga taatatcggc aggattctgg gaaagtttac gtctttttac
    11401 tgcccgggat ggcggttgac ataagctgca ggcaaagctg ccaacaggct ggtgagcgtg
    11461 ggtaataaaa ttgccgccgc agcagttgca gctggaaagt tgcagtaatc cactttcaac
    11521 aaaccgcacc aatgtccagg cacgggttaa tgccagcagt ggtccttctt ctgcttgtgg
    11581 gcactgttca aggtataaac ggtaggcttt gatcaccgca tcgacgccat tacacaaacc
    11641 ggttttcagt aaaaactgcc atgcattaca gaacatcgaa gcatgaacgt tttgttccca
    11701 ggtcataaac cagtcggttg agaatggcag catgcctttc ggcggtgggc ttccgcgcag
    11761 ttctttataa agttttatca ggcgtccgcg acttaactgt gtttcgcttt ccagcatctg
    11821 caaacgagcg cccagggtga tcaattccat tgccagctga atatcccgcg cttcctgaac
    11881 aatgcttttt tcactcatga tcaggccctt ttcttgcgca gcgcttcttc aggctgatta
    11941 acatcattca gcaagcgtgt tgagagcatg atgccggtat gaatttgctg gagatcgtca
    12001 acgcgggaat cttgcgtcaa ctgagtaatc gtctggtggc tgtcaaaacg gaagtgacaa
    12061 accagctgat tggtttctgc cagcttaacc atttgcggaa ga
//
LOCUS       DAJTRC010000107        11773 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.107, whole genome shotgun sequence.
ACCESSION   DAJTRC010000107 DAJTRC010000000
VERSION     DAJTRC010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11773)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11773)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11773
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.107"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..190)
                     /locus_tag="OO357_003295"
     CDS             complement(<1..190)
                     /locus_tag="OO357_003295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding response regulator"
                     /protein_id="HCR5888135.1"
                     /translation="MQIVMFDRQSIFIHGIKISLQQRIPGVSIQGVSQADELWQKLES
                     YPEALVMLDGDQDGEFCYW"
     gene            710..1609
                     /locus_tag="OO357_003296"
     CDS             710..1609
                     /locus_tag="OO357_003296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417978.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5888136.1"
                     /translation="MDKIHAMQLFIKVAELESFSRAADFFALPKGSVSRQIQALEHQL
                     GTQLLQRTTRRVKLTPEGMTYYQRAKDVLSNLSELDGLFQQDATSISGKLRIDIPSGI
                     AKNLLLPRLSEFLYQHPGIELELSSHNRPVDILHDGFDCVIRTGALPEDGVIARPLGK
                     LTMVNCASPHYLTRFGYPQSPDDLTSHAIVRYTPHLGVHPLGFEVASVNGVQWFKSGG
                     MLTVNSSENYLAAGLAGLGIIQIPRIAVREALRAGRLIEVLPGYRAEPLSLSLVYPQR
                     RELSRRVNLFMQWLAGVMKEHLD"
     gene            1658..2671
                     /gene="yhjD"
                     /locus_tag="OO357_003297"
     CDS             1658..2671
                     /gene="yhjD"
                     /locus_tag="OO357_003297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YhjD"
                     /protein_id="HCR5888137.1"
                     /translation="MTQENEIKRPTQDLEHEPIKQLDNSEKGGKVSQALETVTTTAEK
                     VQRQPVIAHLIRATERFNDRLGNQFGAAITYFSFLSMIPILMVSFAAGGFVLASHPML
                     LQDIFDKILQNISDPTLAATLKNTINTAVQQRTTVGLVGLAVALYSGINWMGNLREAI
                     RAQSRDVWERSPQDQEKFWVKYLRDFISLIGLLIALIVTLSITSVAGSAQQMIISALH
                     LNSIEWLKPTWRLIGLAISIFANYLLFFWIFWRLPRHRPRKKALIRGTFLAAIGFEVI
                     KIVMTYTLPSLMKSPSGAAFGSVLGLMAFFYFFARLTLFCAAWIATAEYKDDPRMPGK
                     TQP"
     gene            3082..4404
                     /locus_tag="OO357_003298"
     CDS             3082..4404
                     /locus_tag="OO357_003298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MHS family MFS transporter"
                     /protein_id="HCR5888138.1"
                     /translation="MQATATTLDHEQEYTPINSRNKVLVASLIGTAIEFFDFYIYATA
                     AVIVFPHIFFPQGDPTAATLQSLATFAIAFVARPIGSAVFGHFGDRVGRKATLVASLL
                     TMGISTVVIGLLPGYATIGIFAPLLLALARFGQGLGLGGEWGGAALLATENAPPRKRA
                     LYGSFPQLGAPIGFFFANGTFLLLSWLLTDEQFMSWGWRVPFIFSAVLVIIGLYVRVS
                     LHESPVFEKVAKAKKQVKIPLGTLLTKHVRVTVLGTFIMLATYTLFYIMTVYSMTFST
                     AAAPVGLGLPRNEVLWMLMMAVIGFGVMVPVAGLLADAFGRRKSMVIITTLIILFALF
                     AFNPLLGSGNPILVFAFLLLGLSLMGLTFGPMGALLPELFPTEVRYTGASFSYNVSSI
                     LGASVAPYIAAWLQANYGLGAVGLYLAAMAGLTLIALLLTHETRHQSL"
     gene            complement(4586..6646)
                     /locus_tag="OO357_003299"
     CDS             complement(4586..6646)
                     /locus_tag="OO357_003299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HCR5888139.1"
                     /translation="MSKAGKITAAISGAFLLLIVVAIILIATFDWNRLKPTINQKVSA
                     ELNRPFAIRGNLGVVWERQKQETGWRSWVPWPHVHAEDIILGNPPDIPEVTMVHLPRV
                     EATLAPLALLTKTVWLPWIKLEKPDARLIRLSEKNNNWTFNLANDDNKDANAKPSAWS
                     FRLDNILFDQGRIAIDDKVSKADLEIFVDPLGKPLPFSEVTGSKGKADKEKVGDYIFG
                     LKAQGRYNGEPLTGTGKIGGMLALRGEGTPFPVQADFRSGNTRVAFDGVVNDPMKMGG
                     VDLRLKFSGDSLGDLYELTGVLLPDTPPFETDGRLVAKIDTEKSSVFDYRGFNGRIGD
                     SDIHGSLVYTTGKPRPKLEGDVESRQLRLADLGPLIGVDSGKGAEKSKRSEQKKGEKS
                     VQPAGKVLPYDRFETDKWDVMDADVRFKGRRIEHGSSLPISDLSTHIILKNADLRLQP
                     LKFGMAGGSIAANIHLEGDKKPMQGRADIQARRLKLKELMPDVELMQKTLGEMNGDAE
                     LRGSGNSVAALLGNSNGNLKLLMNDGLVSRNLMEIVGLNVGNYIVGAIFGDDEVRVNC
                     AAANLNIANGVARPQIFAFDTENALINVTGTASFASEQLDLTIDPESKGIRIITLRSP
                     LYVRGTFKNPQAGVKAGPLIARGAVAAALATLVTPAAALLALISPSEGEANQCRTILS
                     QMKK"
     gene            complement(6716..7483)
                     /gene="pdeH"
                     /locus_tag="OO357_003300"
     CDS             complement(6716..7483)
                     /gene="pdeH"
                     /locus_tag="OO357_003300"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-guanylate-specific phosphodiesterase"
                     /protein_id="HCR5888140.1"
                     /translation="MIRQVIQRISNPEASIESLQERRFWLQCERAYTWQPIYQTCGRL
                     MAVELLTVVTHPLNPSQRLPPDRYFTEITVSHRMEVVKEQIDLLAQKADFFVEHGLLA
                     SVNIDGPTLIALRQQPKILRQIERLPWLRFELVEHIRLPKDSTFASMCEFGPLWLDDF
                     GTGMANFSALSEVRYDYIKIARELFVMLRQSPEGRTLFSQLLHLMNRYCRGVIVEGVE
                     TPEEWRDVQNSPAFAAQGWFLSRPAPMETLNTAVLAL"
     gene            7715..8644
                     /gene="kdgK"
                     /locus_tag="OO357_003301"
     CDS             7715..8644
                     /gene="kdgK"
                     /locus_tag="OO357_003301"
                     /EC_number="2.7.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygluconokinase"
                     /protein_id="HCR5888141.1"
                     /translation="MSKKIAVIGECMIELSEKGADVKRGFGGDTLNTSVYIARQVDPA
                     ALTVHYVTALGTDSFSQQMLDAWHGENVDTSLTQRMENRLPGLYYIETDSTGERTFYY
                     WRNEAAAKFWLASEQSAAICEELANFDYLYLSGISLAILSPTSREKLLSLLRECRAKG
                     GKVIFDNNYRPRLWASKEETQQVYQQMLECTDIAFLTLDDEDALWGQQPVEDVIARTH
                     NAGVKEVVVKRGADSCLVSIAGEALVDVPAVKLPKEKVIDTTAAGDSFSAGYLAVRLT
                     GGSAENAAKRGHLTASTVIQYRGAIIPREAMPA"
     gene            complement(8740..10236)
                     /locus_tag="OO357_003302"
     CDS             complement(8740..10236)
                     /locus_tag="OO357_003302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5888142.1"
                     /translation="MQGIKIRLLAGGLLMMATAGYVQADALQPDPAWQQGTLSNGLQW
                     QVLTTPQRPSDRVEIRLLVNTGSLAESTQQSGYSHAIPRIALTQSGGLDAAQARSLWQ
                     QGIDPKRPMPPVIVSYDTTLFNLSLPNNRNDLLKEALSYLANATGKLTITPETINHAL
                     QSQDMVATWPADTKEGWWRYRLKGSTLLGHDPADPLKQPVEAEKIKDFYQKWYTPDAM
                     TLLVVGNVDARSVVDQINKTFGELKGKRETPAPVPTLSPLRAEAVSIMTDAVRQDRLS
                     IMWDTPWQPIRESAALLRYWRADLAREALFWHVQQALSASDSKDIGLGFDCRVLYLRA
                     QCAINIESPNDKLNSNLNLVARELAKVRDKGLPEEEFNALVAQKKLELQKLFAAYARA
                     DTDILMGQRMRSLQNQVVDIAPEQYQKLRQDFLNSLTVEMLNQDLRQQLSNDMALILL
                     QPKGEPEFNMKALQAAWDQIMAPSTAAATTSVATDDVHPEVTDIPPAQ"
     gene            complement(10457..11743)
                     /gene="dctA"
                     /locus_tag="OO357_003303"
     CDS             complement(10457..11743)
                     /gene="dctA"
                     /locus_tag="OO357_003303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C4-dicarboxylate transporter DctC"
                     /protein_id="HCR5888143.1"
                     /translation="MKTSLFKSLYFQVLTAIAIGILLGHFYPEIGEQMKPLGDGFVKL
                     IKMIIAPVIFCTVVTGIAGMESMKAVGRTGAVALLYFEIVSTIALIIGLIIVNVVQPG
                     AGMNVDPATLDAKAVAVYADQAKDQGIVAFIMDVIPASVIGAFASGNILQVLLFAVLF
                     GFALHRLGSKGQLIFNVIESFSQVIFGIINMIMRLAPIGALGAMAFTIGKYGVGTLVQ
                     LGQLIICFYITCILFVVLVLGSIAKATGFSIFKFIRYIREELLIVLGTSSSESALPRM
                     LDKMEKLGCRKSVVGLVIPTGYSFNLDGTSIYLTMAAVFIAQATNSQMDIVHQITLLI
                     VLLLSSKGAAGVTGSGFIVLAATLSAVGHLPVAGLALILGIDRFMSEARALTNLVGNG
                     VATIVVAKWVKELDHKKLDDVLNNRAPDGKTHELSS"
BASE COUNT         2825 a         3191 c         2912 g         2845 t
ORIGIN      
        1 accagtagca aaactcacca tcctgatcgc catcgagcat aactaaggct tcagggtaac
       61 tttccagctt ttgccataac tcgtctgcct gactgacccc ctgaatactc actcctggaa
      121 tacgctgctg taaactgatt tttattccat gaataaatat tgactgcctg tcaaacatga
      181 ctatttgcat aactgaatct ccacctgaat acgttaaaaa gacttaagta gtggaagggt
      241 attacccgcg agaaaaaata agaattcgcc atttggcggt ggccattcta cagagatgac
      301 gcgtagaaaa tagttaccga tataaatagt tacagctaaa cgcctgaaat tacatgtcga
      361 gggcactatt taaaacaatt ttgaggattt ccttatattg gtggttagta cgcatgcaat
      421 taaaaatgaa attccgcgac tacaagccaa aataacaaat ggcaatgaga caaaaataag
      481 cacaaatagc caatacgtcc tctattcact ttaaagggaa tcactgaaaa atacgctctg
      541 tttaagggga ttcacctttc tcataaagct attccgccct tttcctgctg agaaatcgcc
      601 acattcgtca tgacaacatt gtgaaacccg gcattagatg ttagaaaaaa caataaacaa
      661 tgcgatatgc gcgttactgt ttcgctgtga acaacaatcg gtcaaagaaa tggataaaat
      721 tcacgcaatg cagttgttca tcaaagtcgc ggagctggaa agtttttccc gcgcggcgga
      781 tttctttgct ttgccaaagg gaagtgtttc gcgccagata caggcactgg aacatcaact
      841 tggcacccag cttctccagc gcactacgcg acgggtcaaa ctcacgccag aaggcatgac
      901 ctattatcaa cgggcaaaag atgtgttgag taatctcagc gaactggacg gtctgtttca
      961 acaggatgcc accagtatca gcggtaaatt acgcatcgac atcccgtcag gaatcgcgaa
     1021 aaacctgtta ctgccgcgtc tgtcggaatt tctctatcag catccgggaa ttgagctgga
     1081 actgagtagc cataaccgtc cggtagatat tcttcatgat ggttttgatt gcgtgatacg
     1141 cactggcgcg ttaccggaag atggcgttat cgcccgtccc ctcggcaaac taaccatggt
     1201 caactgtgcc agtccgcact atctgacgcg ctttggttat ccgcaaagcc ccgatgatct
     1261 gacttcacac gcaatagtgc gttatacacc gcacctgggt gtacatccgt taggttttga
     1321 ggttgccagc gttaatggcg tccagtggtt taagtctggc ggcatgttga cggtaaacag
     1381 tagcgaaaac tatctcgccg ccggtcttgc cggtctgggg attattcaga tcccgcgcat
     1441 tgccgtgcgc gaagccctgc gtgccgggcg gcttattgaa gtgttgccag gctaccgtgc
     1501 cgagccgctc tccctttcgc tggtttaccc gcagcgtcgg gagctttccc ggcgtgtaaa
     1561 cctgtttatg cagtggctgg ctggcgtaat gaaagagcac ctggactgac cgactatact
     1621 ttttaagaat gaccacgaca gaaggacaaa agagcggatg acgcaggaaa acgagatcaa
     1681 acgtcccacc caggatctgg agcacgagcc gattaagcag ttggataata gcgagaaagg
     1741 cggcaaagtt agccaggcgc tggaaaccgt caccaccact gccgaaaaag tccaacgcca
     1801 accggttatt gcgcacctta ttcgcgcgac agaacgcttt aacgatcggc tgggtaacca
     1861 gtttggtgcg gctatcacct atttctcatt tttgtcgatg atcccgattt tgatggtgtc
     1921 gtttgccgca gggggctttg tgctggcctc ccatccgatg ttgctacagg atatcttcga
     1981 caaaattctg caaaacatca gcgatccgac actagccgcc acgttgaaaa acaccatcaa
     2041 caccgccgtt cagcagcgta cgactgtagg gcttgtcggc ctggcggtgg cactttattc
     2101 cggcatcaac tggatgggta acctgcgtga agcgattcgt gcccagtcgc gcgatgtctg
     2161 ggaacgctcg ccgcaagatc aggagaagtt ctgggtaaaa tatctgcgtg attttatttc
     2221 actgattggt ttgttgattg cgctgattgt gacgctttcg atcacctcgg ttgccggttc
     2281 ggcgcagcaa atgattatta gcgccctgca cctgaacagc attgagtggc tgaaaccgac
     2341 gtggcgattg attggcctgg cgatttccat cttcgccaac tatctgcttt tcttctggat
     2401 cttctggcgg ttgccgcgcc accgtccgcg caaaaaagcc ctgattcgcg gaacatttct
     2461 ggctgctatt ggttttgaag tgattaaaat cgtgatgacc tacaccctgc catcgctgat
     2521 gaaatccccc tctggcgcag catttggttc cgtgctgggg ctgatggcat ttttctactt
     2581 cttcgcccgt ttgacgctgt tttgcgcggc gtggattgcc accgccgaat ataaagacga
     2641 cccgcgaatg ccagggaaaa cgcagcctta aaataggccg gatgcggcgt aaacgcctta
     2701 tccggcatac gattttattc ggcctggctc cccgtaggcc ggataagatg cgccagcatc
     2761 gcatccggct ataatgcgca cataacctct tgaaactctt ccccagagcc tcttcagcca
     2821 tctatttggg agcaaacaat ttcactccaa ctcataaccc cagcatataa atccagttgg
     2881 taacttttat ttaacctgaa accagtttta tccactattt ataaaattat gtgaagcatt
     2941 tcatagaaga aaaatcactg gcctaaacat tatccccttt ttgcctggtt tttgaccatt
     3001 tccgcgattt gttacacatt gaaatatcac ttttgctgtg cgtaatatgg ctattcgtta
     3061 gccaaaaaat aagaaaagat tatgcaagca acagccacaa cactcgacca cgaacaagaa
     3121 tacacgccga tcaactcgcg taataaagtc cttgtcgcct ctctcattgg cacggccatt
     3181 gagttcttcg acttttacat ttacgccact gcggccgtta ttgtgtttcc gcatatcttc
     3241 ttcccgcagg gcgatccaac ggcagcaacg ctacagtcgc tcgccacctt cgccatcgcc
     3301 ttcgtcgcgc gccccattgg ctctgccgtt tttggtcatt ttggcgatcg cgttgggcgt
     3361 aaagcgacgc tggtcgcctc gttgctaacg atggggattt cgaccgtggt gattggtctg
     3421 ctgccgggct atgccacgat tggtattttc gccccactgc tgctggcgct ggctcgattt
     3481 ggtcagggtc tgggcttagg cggtgaatgg ggcggcgcgg cgctgctggc gactgaaaac
     3541 gccccaccgc gcaaacgtgc actgtatggc tccttcccac agctaggcgc accgattggc
     3601 ttcttctttg ccaacggcac tttcttgctg ctttcctggc tactgaccga tgagcagttt
     3661 atgagctggg gctggcgtgt gccatttatc ttctcggcgg tgctggtcat tatcggcctg
     3721 tatgttcgcg tgtcgctgca tgagtcgccg gtgtttgaga aagtcgctaa agcgaaaaaa
     3781 caggtgaaga tcccgctggg tacgctgctg accaaacatg ttcgcgtaac cgtactgggt
     3841 acgttcatta tgctggcaac ctatacgctg ttttacatca tgacggtcta ctctatgacc
     3901 ttcagtaccg ccgccgcgcc agttgggctt ggcctgccgc gtaacgaagt gttgtggatg
     3961 ctgatgatgg cagttattgg ttttggcgtg atggtgccag tcgccggatt actggctgat
     4021 gcctttggtc gccgtaaaag catggtgatc atcaccacgc tgatcatcct gttcgcgctg
     4081 ttcgccttta acccgctgct cggttctggc aacccgattc tggtttttgc cttcctgctg
     4141 ctggggttaa gtctgatggg gctgaccttc gggccgatgg gtgcgctgtt accagagctg
     4201 tttccgacag aagtgcgtta caccggagca tcgttctctt acaacgtatc gtcgattctc
     4261 ggggcttccg ttgcgccgta tatcgcagcc tggttgcagg ctaactacgg gttaggtgcg
     4321 gtggggttat atctggcggc gatggctggc ttgacgttaa tcgccctgct gctgacccat
     4381 gagacgcgac atcagtcgtt gtaataagtg tgaaaagcct gatgcgctac gcttatcagg
     4441 actgaatttc catacaatat attgaattca caaggatttg taggccggat aaggcgttca
     4501 cgccgcatcc ggcatgaaca aagcacactt tgccaaaaat ctaaatctgt ttaattgccg
     4561 gatgtcagac atccggcaat tatcatcact tcttcatctg cgacaatatc gtccgacact
     4621 gattagcctc cccttcggaa ggtgagatca gtgccagtaa cgccgccgcc ggtgttacca
     4681 gcgttgccag tgccgcagca acagcaccac gggcaatcag cggtccggct ttcaccccag
     4741 cctgcggatt tttaaacgtc ccacgcacat acagcggcga acgcagtgtg ataatccgaa
     4801 tccctttact ctccggatca atagtcaaat ccagctgttc cgaagcaaaa cttgccgtgc
     4861 cggtaacatt aatcaacgcg ttctcagtat cgaaagcaaa aatctgcggg cgcgccacgc
     4921 cgttggcaat attcagattc gccgccgcgc agttcacccg cacctcatcg tcaccaaata
     4981 tcgcaccgac aatgtagttg ccgacattca gcccaacaat ctccatcagg ttgcggctca
     5041 ccagcccgtc attcatcaac agtttcaggt tgccgttact gttgcctaaa agcgccgcca
     5101 ccgagttacc gctaccgcgt agttccgcgt caccgttcat ttcccccagc gtcttctgca
     5161 tcagttccac atcgggcatc agttctttca gtttcagtcg acgagcctga atatctgccc
     5221 gcccctgcat cggcttttta tcgccttcca gatgaatatt cgccgcaatg ctgccgcccg
     5281 ccatgccaaa tttcagcggt tgcaggcgca ggtcagcatt tttgaggatg atatgagtag
     5341 aaagatcgct aatcggcagg ctactgccat gctcaatgcg ccgccctttg aagcgaacat
     5401 cggcatccat aacgtcccat ttatcggttt cgaagcggtc ataaggcagc actttgcccg
     5461 caggctgaac gcttttttcg cccttcttct gttcagaccg tttcgacttt tctgcccctt
     5521 tcccggaatc aacgccaatc aacggtccca ggtccgccag ccgcaattgc cgcgactcga
     5581 catcaccttc cagttttggt cgtggctttc cggtggtgta gaccagagaa ccgtggatat
     5641 cgctatcgcc aattcgccca ttaaaaccgc gataatcaaa gaccgacgat ttttcagtgt
     5701 cgattttcgc taccagccga ccatccgttt caaacggcgg ggtatcgggc agcagaacgc
     5761 ccgtcagttc atagagatca cccagtgaat cgccagaaaa tttaagccgt aaatcgacac
     5821 cgcccatctt cattgggtca ttcacgacgc catcaaaagc aacacgggta ttacctgaac
     5881 ggaaatcagc ctgtaccgga aacggcgtcc cttcgccacg cagcgccagc ataccgccta
     5941 tttttcccgt acccgtgagc ggttcaccgt tataacgtcc ctgcgccttc aggccaaaaa
     6001 tgtaatcgcc caccttttct ttatccgctt tacctttcga tccagtaact tcgctgaatg
     6061 gcagcggctt gcctaacgga tcaacaaaaa tttccagatc cgctttgctt actttgtcat
     6121 caatggcgat ccgcccttga tcgaaaagaa tattatccag ccgaaacgac catgccgacg
     6181 gctttgcatt cgcgtctttg ttgtcatcgt tggcaagatt aaacgtccag ttattgttct
     6241 tttcagagag gcgaatcagg cgcgcgtcgg gcttttcgag cttgatccac ggcagccaga
     6301 ccgttttggt cagcagcgcc agcggtgcca gcgttgcctc tactcgcggc aaatgcacca
     6361 tcgtgacttc gggaatatcc ggtggattac caagaatgat gtcttctgca tgtacatgag
     6421 gccacggcac ccagctgcgc cagccagttt cttgtttttg ccgctcccac accacgccca
     6481 gattgccacg gatagcgaac ggacgattca actccgcaga gactttctgg ttgatggtcg
     6541 gtttgagtcg attccagtca aaagttgcaa tcaaaatgat cgccacgaca atcaacaaca
     6601 agaaagcccc tgaaatcgca gcggttattt tgcctgcctt gctcatcgtt cgcttcctca
     6661 tattcttcct gtgccagtcc taaagatagt ccagcccggc ggaaaatgag gcagcttata
     6721 gcgccagaac cgccgtattc agcgtttcca tcggtgccgg gcgtgaaaga aaccagcctt
     6781 gtgcggcgaa tgcgggcgag ttctgaacat cacgccactc ttccggtgtt tctacgccct
     6841 cgacaatcac cccgcgacaa tagcgattca ttagatgtaa aagctgagaa aagagtgtgc
     6901 ggccttccgg cgactgacgc agcatcacaa acagttctcg cgcgattttg atgtagtcat
     6961 aacgcacttc acttagtgca gagaaatttg ccatcccggt accaaaatca tccagccaca
     7021 gcgggccaaa ttcacacatc gaggcaaagg ttgaatcttt cggcagacgg atatgctcca
     7081 ccagttcgaa acgcagccag ggaagacgct caatctggcg caggattttt ggttgctgac
     7141 gcagggcgat gagcgtaggg ccatcaatat tgaccgatgc cagcaggccg tgctctacaa
     7201 agaagtcggc tttttgcgcc agcaaatcaa tctgctcttt cacaacctcc atccgatggc
     7261 tgacggtgat ttcagtaaaa tagcgatccg gcggcaggcg ttgcgaaggg ttcaagggat
     7321 gcgtgaccac cgttaatagc tccacggcca ttaaccgccc gcatgtttga tagatcggct
     7381 gccaggtgta agcacgctca cactgcaacc aaaaacgccg ttcctgcaag ctctcgatgc
     7441 ttgcttcagg gttgcttatt cgctggataa cctgccttat catcaaagat gtcctgtgtt
     7501 taaaagtgat gctgcccgga ctcgacaaag attatcggcg cgtaagggca gaactttacc
     7561 gcctggccgg ggtaaatttc ctcactccac aacggaatct ttcaggaata cgttctggct
     7621 caaaaaaata atggaacact gttttaatat ggttgaccag caaaccacca cagcgcaaac
     7681 taacgctaat tttttacaga tcaggttcac gactatgtcc aaaaagattg ccgtgattgg
     7741 cgaatgcatg attgagcttt ccgagaaagg cgcggacgtt aagcgcggtt tcggcggcga
     7801 taccctgaac acttccgtct atatcgcccg tcaggtcgat cctgcggcat taaccgttca
     7861 ttacgtaacg gcgctgggaa cggacagttt tagccagcag atgctggacg cctggcacgg
     7921 cgagaacgtt gatacttccc tgacccaacg gatggaaaac cgtcttccgg gcctttacta
     7981 cattgaaacc gacagcaccg gcgaacgtac gttctactac tggcgtaacg aagccgccgc
     8041 caaattctgg ctggcgagcg agcagtctgc ggcgatttgc gaagagctgg cgaatttcga
     8101 ttatctctac ctgagcggga ttagcctggc gatcttaagc ccgaccagcc gcgaaaagct
     8161 gctttccctg ctgcgcgaat gccgcgccaa aggcggaaaa gtgattttcg acaataacta
     8221 tcgtccgcgc ctgtgggcca gcaaagaaga gacacagcag gtgtaccagc aaatgctgga
     8281 atgcacggat atcgccttcc tgacgctgga cgacgaagac gcgctgtggg gtcaacagcc
     8341 ggtggaagac gtcattgcgc gcacccataa cgcgggcgtg aaagaagtgg tggtgaaacg
     8401 cggggcggat tcttgcctgg tgtccattgc tggtgaagcg ttagttgatg ttccggcggt
     8461 gaaactgccg aaagaaaaag tgatcgatac caccgcagct ggcgactctt tcagcgccgg
     8521 ttatctggcg gtacgtttga cgggcggcag tgcggaaaac gcggcgaaac gcgggcacct
     8581 gaccgcaagt accgttattc agtatcgcgg cgcgattatc ccgcgtgagg cgatgccagc
     8641 gtaaacgcag catatgcaaa aggctggata aagtgttacc cctctatcca gctcatccat
     8701 aaatacctga tggttgtgct aactcatcac catcaggcat tactgtgcag gtggaatatc
     8761 cgtcacttca ggatgtacgt catccgtggc gacagaggtg gtcgcagcgg cggtagatgg
     8821 ggccatgatt tgatcccagg ccgcctgcaa cgctttcatg ttaaattccg gctcgccttt
     8881 cggctgcagc agtattaacg ccatatcatt cgacaactgc tgacgcagat cctgatttaa
     8941 catctccaca gtcaggctgt taaggaaatc ctgccgcagt ttctgatact gctccggcgc
     9001 gatatcgacg acctgatttt gcaacgaacg catccgctgg cccatcagaa tatcggtatc
     9061 agcccgtgca taggcggcaa acagtttctg cagctccagt ttcttttgcg ccactaacgc
     9121 attgaactct tcttccggca gacctttatc gcgaaccttc gccagttcac gcgccaccag
     9181 attaaggttg ctgttcagct tgtcgtttgg tgattcgatg ttgatggcac actgcgcacg
     9241 cagatacagc acacggcagt caaatccaag accgatgtct ttgctgtcac tggcgcttaa
     9301 cgcttgctga acatgccaga acagcgcctc gcgggccagg tccgcacgcc agtagcgcag
     9361 cagtgcggct gattcacgaa tcggctgcca cggcgtatcc cacatgatag ataaccggtc
     9421 ctgacgcacc gcgtcagtca taatactcac cgcttccgca cgcagcggag aaagcgtcgg
     9481 caccggagct ggcgtttcac gtttgccttt cagttcgcca aacgttttgt tgatttggtc
     9541 gacaaccgag cgcgcatcca cgtttcccac caccagtagc gtcattgcat ccggggtgta
     9601 ccatttctga tagaaatctt taattttttc cgcttcaacg ggttgtttca gcggatcggc
     9661 aggatcgtga cctaacaagg ttgacccttt cagacgatag cgccaccagc cctctttagt
     9721 atcggcaggc caggttgcca ccatgtcctg actttgcagc gcgtggttga tggtttctgg
     9781 tgtgatggtc aatttgccag tggcatttgc cagataagag agcgcttctt tcagcaaatc
     9841 gttacggtta ttgggcaaac tcagattaaa cagcgtggtg tcataagaga caattaccgg
     9901 cggcatcggg cgtttagggt cgatcccctg ctgccacaat gaacgcgcct gtgctgcgtc
     9961 aaggccaccg ctttgcgtta gcgcaatacg agggatggcg tgactgtaac cgctctgttg
    10021 tgtactttcg gcgagcgaac cggtattaac cagcaggcga atttcaacac gatcgctggg
    10081 acgctggggg gtggtcagca cttgccactg taaaccgttg gaaagcgtcc cctgttgcca
    10141 tgctggatca ggctggagcg catctgcctg cacatagcca gcagtggcca tcatcagcaa
    10201 accgcccgct aaaagtcgaa tttttatgcc ctgcatgtga acccctgatc aactatcctg
    10261 gtaataaaaa aactgcccgt tgtcggtcag tctaaatgac gttaaaaaca cttcgtgtta
    10321 gaccgcaaga acatgaaaat gtcacggaag aagtgaaata aacctgaact cacccaggtg
    10381 gtaagttcag gcacaggggt caattatgcg caaacacccg cactcgggga agggagtgcg
    10441 ggcataagtg gtgagattaa gaggataatt cgtgcgtttt gccatccggc gcacgattat
    10501 tcagcacatc gtccagtttt ttgtggtcca gttctttcac ccacttagca acgacaatgg
    10561 tcgctacgcc gttaccgacc aggttagtta gcgcacgagc ttctgacata aagcggtcga
    10621 taccgaggat cagcgccaga cccgctaccg gcaaatggcc caccgcagag agcgtcgccg
    10681 ccagcacgat aaagccacta cccgttaccc ctgccgcccc tttagaagaa agcagcaaca
    10741 cgattaacag cgtgatttgg tggacgatat ccatctgact gttagtggcc tgggcgataa
    10801 acaccgccgc cattgtcagg tatatcgatg tgccatcaag gttaaacgag tagcctgtcg
    10861 ggatgaccag ccccaccacc gatttacggc agccgagttt ctccatcttg tcgagcatac
    10921 gcggcagcgc cgactcggaa gatgaagtcc ccagtacaat cagcagttct tcacggatgt
    10981 agcggataaa tttgaagata ctgaaaccag tcgctttagc gattgaaccc aataccagca
    11041 ccacaaacag gatacaggta atgtagaaac agataatcag ctgccccagt tgcaccagtg
    11101 tgccgacgcc gtatttaccg atggtaaacg ccattgcccc gagcgcacca ataggtgcca
    11161 gacgcatgat catattgatg atgccgaaga tgacctgcga gaaactttcg atcacgttaa
    11221 aaatcagttg gcctttgctg cccagacggt ggagcgcaaa accaaacagt acggcaaaca
    11281 gcagcacctg cagaatgtta ccgctggcaa atgcgccaat gacgctcgcc gggatgacat
    11341 ccataatgaa ggcgacaatg ccctggtctt tcgcctgatc ggcgtaaacc gctaccgctt
    11401 tcgcatcaag cgttgccgga tcgacgttca ttccggcacc aggctgcacg acgttaacga
    11461 tgataagacc aataatcagc gcgatggtac tgacaatttc aaagtaaagc agtgcgactg
    11521 cgccggtacg accgaccgcc ttcatgcttt ccatgcccgc aatgcccgtt acgacggtac
    11581 aaaagatgac aggagcgatg atcatcttaa tgagcttaac gaagccgtcg ccaagcggtt
    11641 tcatttgctc gcctatttca ggatagaaat ggccaaggag aataccaatg gctatcgctg
    11701 tcaggacctg aaagtaaagg cttttaaaca gagaggtttt catagggtgt cctttagtaa
    11761 aaccacaggt ctt
//
LOCUS       DAJTRC010000108        11682 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.108, whole genome shotgun sequence.
ACCESSION   DAJTRC010000108 DAJTRC010000000
VERSION     DAJTRC010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.108"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..346
                     /locus_tag="OO357_003304"
     CDS             <1..346
                     /locus_tag="OO357_003304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418608.6"
                     /note="catalyzes the formation of 3-methylcrotonyl-CoA
                     from isovaleryl-CoA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5888144.1"
                     /translation="GNIMCLDVLRVLNKQAGVYDLLSEAFVEVKGQDRYFDRAVRRLQ
                     QQLRKPAEELGREITHQLFLLGCGAQMLKYASPPMAQAWCQVMLDTRGGVRLSEQIQN
                     DLLLRATGGVCV"
     gene            complement(463..738)
                     /gene="yjfN"
                     /locus_tag="OO357_003305"
     CDS             complement(463..738)
                     /gene="yjfN"
                     /locus_tag="OO357_003305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease activator YjfN"
                     /protein_id="HCR5888145.1"
                     /translation="MKQLLASPSLQLVTYPASATAQSAEFASADCVTGLNEIGQISVS
                     NISGDPQDVERIVALKADEQGASWYRIITMYEDQQPDNWRVQAILYA"
     gene            complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="OO357_003306"
     CDS             complement(887..1216)
                     /gene="bsmA"
                     /locus_tag="OO357_003306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313192.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biofilm peroxide resistance protein BsmA"
                     /protein_id="HCR5888146.1"
                     /translation="MVSRKRNSVIYRFASLLLVLMLSACSALQGTPQPAPPVTDHPQE
                     IRRDQTQGLQRIGSVSTMVRGSPDDALAEIRAKAVAAKADYYVVVMVDETIVTGQWYS
                     QAILYRK"
     gene            1398..2147
                     /gene="yjfP"
                     /locus_tag="OO357_003307"
     CDS             1398..2147
                     /gene="yjfP"
                     /locus_tag="OO357_003307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HCR5888147.1"
                     /translation="MIEIESRELADIPVLHAYPVGQKDTPLPCVIFYHGFTSSSLVYS
                     YFAVALAQAGLRVIMPDAPDHGSRFSGDEARRLNQFWQILLQSMQEFTTLRAAIAEEN
                     WLLDDRLAVGGASMGAMTALGITARHPTVKCTASMMGSGYFTSLARSLFPPLIPETAA
                     QQNEFNKIVAPLAEWEATNHLEQLGDRPLLLWHGLDDDVVPADESLRLQQALSETGRD
                     KLLTCSWQPGVRHRITPEALDAAVTFFRQHL"
     gene            complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="OO357_003308"
     CDS             complement(2144..2899)
                     /gene="ulaR"
                     /locus_tag="OO357_003308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710056.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator UlaR"
                     /protein_id="HCR5888148.1"
                     /translation="MTEAQRHQILLEMLAQLGFVTVEKVVERLGISPATARRDINKLG
                     ESGKLKKVRNGAEAITQQRPRWTPMNLHQAQNHDEKVRIAKAASQLVNPGESVVINCG
                     STAFLLGREMCGKPVQIITNYLPLANYLIDQEHDSVIIMGGQYNKSQSITLSPQGSEN
                     SLYAGHWMFTSGKGLTAEGLYKTDMLTAMAEQKMLSVVGKLVVLVDSSKIGERAGMLF
                     SRADQIDMLITGKNANPEILQQLEAQGVSILRV"
     gene            complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="OO357_003309"
     CDS             complement(3007..4071)
                     /gene="ulaG"
                     /locus_tag="OO357_003309"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003856017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HCR5888149.1"
                     /translation="MSKVKSITRESWILSTFPEWGSWLNEEIEQEQVAPGTFAMWWLG
                     CTGIWLKSEGGTNVCVDFWCGTGKQSHGNPLMKQGHQMQRMAGVKKLQPNLRTTPFVL
                     DPFAIRQIDAVLATHDHNDHIDVNVAAAVMQNCADDVPFIGPKTCVDLWIGWGVPKER
                     CIVVKPGDVVKVKDIEIHALDAFDRTALITLPADQKAAGVLPDGMDDRAVNYLFKTPG
                     GSLYHSGDSHYSNYYAKHGNEHQIDVALGSYGENPRGITDKMTSADMLRMGEALNAKV
                     VIPFHHDIWSNFQADPQEIRVLWEIKKDRLKYGFKPFIWQVGGKFTWPLDKDNFEYHY
                     PRGFDDCFTIEPDLPFKSFL"
     gene            4426..5823
                     /gene="ulaA"
                     /locus_tag="OO357_003310"
     CDS             4426..5823
                     /gene="ulaA"
                     /locus_tag="OO357_003310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418614.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HCR5888150.1"
                     /translation="MEILYNIFTVFFNQVMTNAPLLLGIVTCLGYILLRKSVSVIIKG
                     TIKTIIGFMLLQAGSGILTSTFKPVVAKMSEVYGINGAISDTYASMMATIDRMGDAYS
                     WVGYAVLLALALNICYVLLRRITGIRTIMLTGHIMFQQAGLIAVTLFIFGYSMWTTII
                     CTAILVSLYWGITSNMMYKPTQEVTDGCGFSIGHQQQFASLIAYKVAPFLGKKEESVE
                     DLKLPGWLNIFHDNIVSTAIVMTIFFGAILLSFGIDTVQAMAGKVHWTVYILQTGFSF
                     AVAIFIITQGVRMFVAELSEAFNGISQRLIPGAVLAIDCAAIYSFAPNAVVWGFMWGT
                     IGQLIAVGILVACGSSILIIPGFIPMFFSNATIGVFANHFGGWRAALKICLVMGMIEI
                     FGCVWVVKLTGMSAWMGMADWSILAPPMMQGFFSIGIAFMAVIIVIALAYMFFAGRAL
                     RAEEDAEKQLAEQSA"
     gene            5839..6144
                     /gene="ulaB"
                     /locus_tag="OO357_003311"
     CDS             5839..6144
                     /gene="ulaB"
                     /locus_tag="OO357_003311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710059.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIB"
                     /protein_id="HCR5888151.1"
                     /translation="MTVRILAVCGNGQGSSMIMKMKVDQFLTQSNIDHTVNSCAVGEY
                     KSELSGADIIIASTHIAGEITVTGNKYVVGVRNMLSPADFGPKLLEVIKAHFPQDVK"
     gene            6154..6618
                     /gene="ulaC"
                     /locus_tag="OO357_003312"
     CDS             6154..6618
                     /gene="ulaC"
                     /locus_tag="OO357_003312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIA"
                     /protein_id="HCR5888152.1"
                     /translation="MKLHDSLAENKSIRLQAEAETWQDAVKIGVDLLVAADVVEPRYY
                     QAILDAVEQHGPYFVLAPGLAMPHGRPEEGVKKTGFALVTLKKPLEFNHEDNDPVDIL
                     ITMAAVDANTHQEVGIMQIVNLFEDEENFDRLRACRTEQEVLDLIDRTNAAA"
     gene            6632..7282
                     /gene="ulaD"
                     /locus_tag="OO357_003313"
     CDS             6632..7282
                     /gene="ulaD"
                     /locus_tag="OO357_003313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HCR5888153.1"
                     /translation="MSLPMLQVALDNQTMDSAYETTRLIAEEVDIIEVGTILCVGEGV
                     RAVRDLKALYPHKIVLADAKIADAGKILSRMCFEANADWVTVICCADINTAKGALDVA
                     KEFNGDVQIELTGYWTWEQAQQWRDAGIQQVVYHRSRDAQAAGVAWGEADITAIKRLS
                     DMGFKVTVTGGLALEDLPLFKGIPIHVFIAGRSIRDAASPVEAARQFKRSIAELWG"
     gene            7292..8146
                     /gene="ulaE"
                     /locus_tag="OO357_003314"
     CDS             7292..8146
                     /gene="ulaE"
                     /locus_tag="OO357_003314"
                     /EC_number="5.1.3.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase UlaE"
                     /protein_id="HCR5888154.1"
                     /translation="MLSKQIPLGIYEKALPAGECWLERLRLAKTLGFDFVEMSVDETN
                     ERLSRLDWSREQRLALVNAIVETGVRVPSMCLSAHRRFPLGSEDDAVRAQGLEIMRKA
                     IQFAQDVGIRVIQLAGYDVYYQEANNETRRRFRDGLKESVEMASRAQVTLAMEIMDYP
                     LMNSISKALGYAHYLNNPWFQLYPDIGNLSAWDNDVQMELQAGIGHIVAVHVKDTKPG
                     VFKNVPFGEGVVDFERCFETLKQSGYCGPYLIEMWSETAEDPAAEVVKACDWVKARMA
                     KAGMVEAA"
     gene            8146..8832
                     /gene="ulaF"
                     /locus_tag="OO357_003315"
     CDS             8146..8832
                     /gene="ulaF"
                     /locus_tag="OO357_003315"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710063.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase UlaF"
                     /protein_id="HCR5888155.1"
                     /translation="MQKLKQQVFEANMDLPRYGLVTFTWGNVSAIDRERGLVVIKPSG
                     VAYETMKADDMVVVDMSGKVVEGEYRPSSDTATHLELYRRYPSLGGIVHTHSTHATAW
                     AQAGLAIPALGTTHADYFFGDIPCTRGLSEEEVQGEYELNTGKVIIETLGNAEPLHTP
                     GIVVYQHGPFAWGKDAHDAVHNAVVMEEVAKMAWIARSINPQLNHIDSYLMNKHFMRK
                     HGPNAYYGQK"
     gene            complement(8961..9236)
                     /locus_tag="OO357_003316"
     CDS             complement(8961..9236)
                     /locus_tag="OO357_003316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1471 domain-containing protein"
                     /protein_id="HCR5888156.1"
                     /translation="MFSRVLALLAVLLLSANTWAAIEINNHQARNMDDVQSLGVIYIN
                     HNFATESEARQALNEETDAQGATYYHVILMREPGSNGNMHASADIYR"
     gene            9563..9958
                     /gene="rpsF"
                     /locus_tag="OO357_003317"
     CDS             9563..9958
                     /gene="rpsF"
                     /locus_tag="OO357_003317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020456251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HCR5888157.1"
                     /translation="MRHYEIVFMVHPDQSEQVPGMIERYTAAITGAEGKIHRLEDWGR
                     RQLAYPINKLHKAHYVLMNVEAPQEVIDELETTFRFNDAVIRSMVMRTKHAVTEASPM
                     VKAKDERRERRDDFANETADDAEAGDSEE"
     gene            9965..10279
                     /gene="priB"
                     /locus_tag="OO357_003318"
     CDS             9965..10279
                     /gene="priB"
                     /locus_tag="OO357_003318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal replication protein N"
                     /protein_id="HCR5888158.1"
                     /translation="MTNRLVLSGTVCRTPLRKVSPSGIPHCQFVLEHRSVQEEAGFHR
                     QAWCQMPVIVSGHENQAITHSITVGSRITVQGFISCHKAKNGLSKMVLHAEQIELIDS
                     GD"
     gene            10284..10511
                     /gene="rpsR"
                     /locus_tag="OO357_003319"
     CDS             10284..10511
                     /gene="rpsR"
                     /locus_tag="OO357_003319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012986941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HCR5888159.1"
                     /translation="MARYFRRRKFCRFTAEGVQEIDYKDIATLKNYITESGKIVPSRI
                     TGTRAKYQRQLARAIKRARYLSLLPYTDRHQ"
     gene            10553..11002
                     /gene="rplI"
                     /locus_tag="OO357_003320"
     CDS             10553..11002
                     /gene="rplI"
                     /locus_tag="OO357_003320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HCR5888160.1"
                     /translation="MQVILLDKVANLGSLGDQVNVKAGYARNFLVPQGKAVPATKKNI
                     EFFEARRAELEAKLAEVLAAANARAEKINALETVTIASKAGDEGKLFGSIGTRDIADA
                     VTAAGVEVAKSEVRLPNGVLRTTGEHEVSFQVHSEVFAKVIVNVVAE"
     gene            complement(11073..11636)
                     /locus_tag="OO357_003321"
                     /pseudo
     CDS             complement(11073..11636)
                     /locus_tag="OO357_003321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418625.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
BASE COUNT         2767 a         2871 c         3087 g         2957 t
ORIGIN      
        1 cggcaatatt atgtgcctgg atgtgttgcg cgttctcaat aagcaagcgg gcgtatacga
       61 cttattgtcg gaagcatttg tggaagtgaa agggcaggat cgctattttg atcgcgcggt
      121 tcgtcgttta cagcagcagc tgcgtaagcc agctgaagaa ctggggcgag agattactca
      181 tcagctattc ctgctgggct gcggtgcgca aatgttgaaa tatgcttctc cgccaatggc
      241 gcaggcgtgg tgtcaggtga tgttagatac gcgcggcggc gtacggttgt cagagcagat
      301 ccagaatgat ttattgctgc gggcgacggg gggagtgtgt gtgtaagcgt atacgactga
      361 tgcgacgctg gtttcgatta actaaatgaa atatgtgaaa attgtaggcc ggacaaggcg
      421 ctcgcgccgc atccggcatt gttcatttgc tgtagatgac actcatgcat acagtatcgc
      481 ctgtacgcgc cagttatcag gctgctggtc ttcgtacatg gtaataatgc ggtaccatga
      541 cgcaccttgt tcatcggcct ttaacgccac gatacgttcc acgtcctgcg gatcccctga
      601 aatattgcta actgagatct gacctatttc attcaggccc gtcacgcagt cagcactggc
      661 gaattctgca gactgtgccg tggcgctcgc aggataagtc actaattgca gcgagggtga
      721 ggcaagtaat tgtttcatcg tcagctccat ttttcgtttc cccacatgtt gcagggcatt
      781 ccatcgcggt aataaacgcc caagcttcca gccggtaatt tattgtgcac aggtaaacgc
      841 atagggacgc aaagcaatgt aaagctgact atgtaagttg tctgatttat ttacggtaca
      901 aaatggcttg tgaataccac tgtcctgtca cgatggtttc gtccaccatg acgacaacgt
      961 aataatcagc ttttgcagcg acagcttttg ctctgatttc tgctaatgcg tcatccggag
     1021 aaccccgaac catcgtgctt acgctaccta ttcgctgtaa cccttgcgtc tggtcgcggc
     1081 gaatctcttg cggatggtcc gttactggcg gtgctggctg tggcgtacct tgcagtgcgc
     1141 tacaggcact taacatcaac accaataata aactggcaaa ccggtaaata acgctattac
     1201 gtttcctgct aaccatagtg tagtgcctta ttaatttaac tttgggggaa tgttcccgaa
     1261 ttgttacctg gtacgcgatt ttcgaatgaa aacgtgacga aagcgtaacc cacatctcaa
     1321 cattatgaac taataccgcc agcagaaact tgtgctaagg atttcttcat gtcataccag
     1381 acaaaaagga gagacagatg attgaaatag aatcacgcga gctggcagat attcccgttc
     1441 ttcatgctta tcctgtcggg caaaaagata ccccgttacc gtgcgtaatt ttttatcacg
     1501 gctttacttc atccagcctg gtgtatagct attttgccgt tgcgctggcg caggctggtt
     1561 tgcgggtgat catgccggat gcgcccgatc acggtagccg ttttagtggc gacgaagcgc
     1621 ggcggttaaa tcaattctgg caaatcttgc tacaaagtat gcaggaattc actactttac
     1681 gtgcggcaat agccgaagaa aactggctgc ttgatgaccg tctggcagtc ggtggcgcgt
     1741 cgatgggcgc gatgacggca ctgggaatta ccgctcgcca ccccacggtg aaatgtaccg
     1801 ccagcatgat gggatcgggc tattttacat cgctcgcccg ttcactgttt ccaccgctga
     1861 tacctgaaac ggcagcacag cagaatgaat tcaataaaat tgtcgcgccg ctggcagagt
     1921 gggaagcgac aaaccacctg gaacaacttg gggacagacc tctactgctg tggcatggcc
     1981 tcgacgatga cgttgtgcct gccgacgaat cactacgttt gcagcaggcc ttaagcgaga
     2041 cgggacggga taaactgcta acctgttcat ggcagccagg cgtgcgtcac cgcattacgc
     2101 ctgaggcgtt agatgctgcc gtgacatttt tccgccagca tctttaaaca cgcagaatgc
     2161 tgaccccttg cgcttccagt tgttgcagga tttccgggtt agcatttttg ccggtgatca
     2221 gcatatcgat ttgatcggca cggctaaaaa gcattcccgc gcgttcgcca atcttactgc
     2281 tatcaaccag taccaccagt ttccctacca cgctcagcat cttctgctct gccattgctg
     2341 tcagcatatc ggttttatac aacccttctg cggtcagccc ttttccgctg gtaaacatcc
     2401 agtgcccggc atagagactg ttttcgctgc cctgcgggct taaagtgatg gactgacttt
     2461 tgttgtactg tccacccata atgatcacgc tgtcatgttc ttgatcgatc aggtaatttg
     2521 ccagcggtag ataattagtg atgatttgca ctggcttgcc acacatttcc cgcccaagca
     2581 gaaacgcggt ggagccgcag ttgatgacta cgctttcgcc cggattaacc agctgcgagg
     2641 ccgctttagc aatacgtact ttttcatcgt gattctgcgc ctgatgcaga ttcatcggcg
     2701 tccagcgcgg gcgctgttgg gtaatagctt ctgcgccatt gcgcaccttt ttcagtttgc
     2761 cgctttcgcc aagtttattg atatcgcgtc gcgcagtggc aggcgaaatt cccagacgct
     2821 caacgacttt ctcaacggtc acaaagccca attgtgcgag catttccagg aggatttgat
     2881 gtctttgtgc ttcagtcatg agctattccg ataaaatttg atttttttag atgatatttg
     2941 aaatagccag gaaatactac gccggatagc gcgaacttct ccacgctatc cggtataaac
     3001 gacagattac aggaatgact tgaacggcag atccggttca atagtgaagc aatcatcgaa
     3061 accgcgcgga tagtggtact cgaagttgtc tttatccagc ggccaggtaa atttgccgcc
     3121 aacctgccag ataaacggct tgaagccata cttcagacga tcttttttga tctcccacag
     3181 cacgcggatc tcttgcggat cggcctggaa gtttgaccag atgtcgtggt ggaacgggat
     3241 aaccactttc gcattcaggg cttcacccat acgcagcata tcggcgctgg tcattttgtc
     3301 ggtaataccg cgcgggttct caccatacga tcctaacgcc acatcgatct gatgttcgtt
     3361 accgtgcttc gcataatagt tagagtagtg ggaatcgcca ctgtgatata gggaaccgcc
     3421 aggggttttg aacaggtagt ttactgcgcg atcgtccatg ccatctggca gtacgccagc
     3481 cgctttttga tcggcaggca gggtgatcag tgcagtacgg tcgaaagcgt caagcgcatg
     3541 aatttcaatg tctttcactt ttacaacatc gcccggtttg accacgatgc aacgctcttt
     3601 cggtacgccc cagccaatcc acaaatcaac gcaggttttc ggtccaatga atggtacgtc
     3661 atcagcacag ttctgcatca cggcagcagc gacgttaacg tcgatatgat cgttgtgatc
     3721 gtgagtcgcc agtaccgcgt cgatctggcg aatcgcaaac ggatcaagaa caaacggggt
     3781 ggtgcgtagg tttggctgca gttttttcac gccagccatg cgctgcatct ggtgaccctg
     3841 tttcattaac gggttaccgt gactttgttt gccagtgccg caccagaaat caacgcaaac
     3901 gttggtgcca ccttctgatt tcaaccagat cccggtgcag cctagccacc acatcgcaaa
     3961 tgtgccaggg gcgacctgct cttgttcaat ttcttcattc agccagctac cccactccgg
     4021 gaaagtgctc aggatccagg attctcgggt gatacttttc actttactca tcgccattta
     4081 ccttcatgat agttcaattc gaatcaacat gtgattggtt ttgattaatc ctgacactat
     4141 tccttcagga aggtaatgac cattttttga ctttttgcca gggaagttgt tgttgatttt
     4201 gagtggggaa agatttaatg gaatgtgtag ttaaattaac taaatgaatt taaataaata
     4261 attgtttcgt tgtgtgaatc ccactctatc catgtggaat tatttgcggg tcgcgtcaca
     4321 tttaatcata aataatcttg ttgtgattac ttttgaaaat tagagtgagt gcacaacatt
     4381 ccgggtgtgc ggaatacccg gttacctctt cttcaggaga tcgttatgga gatcctctac
     4441 aacatcttta ccgtgttttt taaccaggtc atgaccaatg ccccgttgtt gctgggtatt
     4501 gtgacctgtc tgggctacat cctactgcgc aaaagtgtca gcgttattat taaaggcacg
     4561 attaaaacca taattggttt catgttgttg caggcagggt ccggcatcct caccagcacc
     4621 ttcaaaccgg tggtggcgaa aatgtccgaa gtctacggca ttaacggcgc aatttccgat
     4681 acttacgcgt cgatgatggc aaccatcgac cgcatgggcg atgcctatag ctgggtaggt
     4741 tacgcggtgt tgttagcgct ggcgctgaac atctgttacg tgctgctgcg tcgcattacc
     4801 ggtattcgta ccatcatgct gactggtcat atcatgttcc agcaggcggg gctgattgcc
     4861 gttacgttgt ttattttcgg ctactccatg tggacaacca ttatctgcac ggcgattctg
     4921 gtttcgctct actggggcat tacctccaac atgatgtaca agccgactca ggaagtgacg
     4981 gacggctgcg gtttctccat cggtcaccag caacagtttg catcattaat tgcctataaa
     5041 gttgcgccgt tcctcggcaa aaaagaggaa agcgttgaag acctcaaact gccaggctgg
     5101 ctgaatattt tccacgacaa catcgtctcc acggcgattg tcatgaccat cttctttggt
     5161 gccattctgc tctccttcgg tatcgacacc gtgcaggcga tggcaggcaa agtgcactgg
     5221 acggtctata tcctgcaaac cggtttttcc ttcgcggtgg cgatcttcat catcactcag
     5281 ggtgtgcgca tgtttgtggc ggaactctct gaagcgttta acggtatctc ccagcgcctg
     5341 atccctggtg cggttctggc gattgactgt gcggctattt atagcttcgc tccgaacgcc
     5401 gtggtctggg gctttatgtg gggcaccatc ggtcagctga ttgcggttgg catcctggtc
     5461 gcctgcggtt cctcgatcct gattatccca ggctttatcc cgatgttctt ctccaacgcc
     5521 accatcggcg tgttcgctaa ccacttcggc ggctggcgtg cggcgctgaa gatttgtctg
     5581 gtgatgggga tgatcgaaat ctttggttgc gtctgggtgg tgaaacttac cggtatgagt
     5641 gcctggatgg ggatggcgga ctggtcgatt ctggcaccgc cgatgatgca gggcttcttc
     5701 tccatcggta tcgcctttat ggccgtcatc attgtaattg cactggctta tatgttcttc
     5761 gctggccgcg cactgcgcgc agaagaagat gcagaaaaac aattggcaga acagtctgct
     5821 taataaggag ttttgattat gaccgtacgt attctggctg tgtgtggcaa cggacaaggc
     5881 agttccatga tcatgaagat gaaagtggac cagtttttaa cccaatcaaa cattgaccac
     5941 acggtaaata gctgcgcggt tggcgaatac aaaagcgagt tgagcggcgc ggatatcatc
     6001 atcgcttcta cgcacattgc gggcgaaatc accgtgaccg gcaacaaata cgtggttggc
     6061 gtgcgcaaca tgctctctcc tgccgacttt ggcccgaaac tgctggaagt gatcaaagcg
     6121 catttcccgc aggatgtgaa gtaaggacgc gccatgaaat tacatgattc gctggcggag
     6181 aataaatcca tccgcctgca ggctgaagct gagacatggc aggacgcagt aaaaatcggc
     6241 gttgacctgc tggtggcggc ggatgttgtc gagccgcgtt actaccaggc gattctggat
     6301 gcggttgaac agcacggtcc gtactttgtg ctcgcaccag gcctggcaat gccgcacggt
     6361 cgcccggaag agggcgttaa gaaaaccggt tttgcgctgg tgacgctgaa aaagccgctg
     6421 gagttcaacc acgaagataa cgacccggtg gatatcctca tcaccatggc ggcggtcgat
     6481 gccaacacgc accaggaagt gggcatcatg cagatcgtca acctgtttga agatgaagaa
     6541 aatttcgacc gtttacgtgc ctgccgtacc gagcaggaag tactggatct cattgaccgc
     6601 accaacgcgg cagcttaaga aggaattgaa gatgtcatta ccgatgttgc aagtcgcgct
     6661 ggacaaccag actatggata gcgcctacga aaccactcgc ctgattgccg aagaagtcga
     6721 cattatcgaa gtgggtacca ttctgtgcgt gggcgaaggc gtgcgtgcgg ttcgcgatct
     6781 gaaagcgctc tacccgcaca aaatcgtgct ggcagacgcc aaaattgccg atgcgggcaa
     6841 aattctttct cgtatgtgct tcgaagccaa cgctgactgg gtgaccgtga tttgttgtgc
     6901 ggatatcaac accgccaaag gtgcgctgga cgtagcaaaa gagtttaacg gcgacgtgca
     6961 gatcgaactg accggttact ggacctggga acaggcgcaa cagtggcgcg acgcaggtat
     7021 tcagcaggtg gtttatcacc gcagccgtga cgcgcaggcc gctggcgtgg cgtggggtga
     7081 agccgatatc actgcgatca aacgtctttc cgatatgggc ttcaaagtca ccgtcaccgg
     7141 tggcctggcg ctggaagatc tgccgctgtt caaaggcatt ccaattcatg tgtttatcgc
     7201 cggtcgcagc atccgtgatg ccgcttctcc ggttgaagcc gcgcgtcagt tcaaacgttc
     7261 catcgccgaa ctgtggggct aaggagcgga tatgttgtcc aaacaaatcc cgcttggcat
     7321 ctatgaaaaa gcgctccccg ccggggagtg ctggctggaa cgcctgcgac tggcaaaaac
     7381 gttgggcttc gattttgtcg agatgtcggt agatgaaact aacgagcgcc tgtcgcgcct
     7441 tgactggagc cgcgaacagc gtctggcgct ggtgaatgct attgttgaaa ccggcgtgcg
     7501 tgtgccgtcc atgtgccttt ctgcccatcg tcgtttcccg ttgggcagcg aagatgacgc
     7561 ggtgcgggcg caggggctgg agattatgcg taaagccatc cagttcgccc aggacgttgg
     7621 cattcgcgtg atccagctgg cgggctatga cgtttactat caggaagcca ataacgaaac
     7681 gcgtcgtcgt ttccgtgacg gcctgaaaga gagcgttgag atggcgagcc gcgcgcaggt
     7741 gacgctggcg atggagatca tggattatcc gttgatgaac tccatcagca aggcgctggg
     7801 ttacgcgcac tatctcaaca acccgtggtt ccagctctac ccggatatcg gcaacctgtc
     7861 ggcgtgggac aacgatgtgc agatggagtt gcaggccgga atcgggcata tcgtcgcggt
     7921 gcatgtgaaa gacaccaaac cgggcgtctt caaaaacgtg ccatttggcg aaggtgtggt
     7981 ggatttcgaa cgttgcttcg aaacgctcaa acagagtggc tattgcgggc cgtacctgat
     8041 tgagatgtgg agcgaaacgg cggaagaccc ggcggcagaa gtggtgaaag cgtgtgattg
     8101 ggtgaaagcg cgcatggcca aagcaggcat ggtggaggcg gcataatgca aaagctaaaa
     8161 cagcaggtgt ttgaagccaa catggatctg ccgcgctatg ggctggtgac ctttacttgg
     8221 ggcaacgtca gcgctatcga ccgcgaacgc gggctggtgg tgatcaagcc cagcggcgtt
     8281 gcctacgaaa ccatgaaagc agacgatatg gtggtggtcg atatgagcgg caaggtggtg
     8341 gaaggggagt atcgcccatc ttccgacact gcgacgcatc tcgaactcta ccgtcgttac
     8401 ccgtcgcttg gcggcattgt ccatacccac tccactcatg ccaccgcatg ggcgcaggcg
     8461 gggctggcga tcccggcatt aggcaccacg cacgctgact acttctttgg tgatatcccg
     8521 tgtacgcgtg gattaagcga agaagaggtg cagggtgagt atgaactgaa cactggcaaa
     8581 gtgattatcg aaacgctggg caacgccgag ccgctgcata cgccgggaat tgtggtgtat
     8641 cagcacggac cgttcgcctg ggggaaagat gcccacgacg cggtgcataa cgcggtagtg
     8701 atggaagaag tggcgaaaat ggcgtggatt gcgcgcagta ttaatccaca actcaatcat
     8761 atcgacagct acctgatgaa taaacacttc atgcgtaagc acggtcctaa cgcttattac
     8821 gggcagaagt aaaacacgcg ctgcggaaat ttccttcctc gggagataac tggtctaatt
     8881 ccgcagccgt ttttcaaaaa aagccccctg cgaagggggc aaagcaaact atggcaatgt
     8941 ttcgttggtt atacctggtg ctagcgataa atatccgcgc tggcgtgcat attgccgtta
     9001 ctccccggtt cccgcatcag aattacgtgg tagtacgttg cgccttgcgc atctgtttct
     9061 tcatttaatg cctgacgtgc ttcgctttca gtggcgaaat tatgattgat ataaatcacg
     9121 cctaagcttt gcacatcgtc catatttctg gcctggtggt tattaatttc aatggctgcc
     9181 catgtatttg cacttagcaa aagcacagcc agaagggcta aaactcgact gaacatagat
     9241 acctcctcga cggctgactt tgtgtgctct cctctgtgat gatcttctga tttaatttta
     9301 atcaatgata aagaagttga tggtgaccat ttctgatgca gttgttcaaa aaaacactgt
     9361 gacggaatgt gatgaacttc aaatcagcgg tttagaggtt atttgcgaaa ggggagattt
     9421 atttctgctc cgcccttgag tttagcgggg tatgccagta ctataacgcg tcatttttca
     9481 gccgaccttt aacacgttcc ttgcctcccc gggattcggc tgacccagac aggaggctga
     9541 ataatccgta aggagcaatt cgatgcgtca ttacgaaatc gtttttatgg tccatcctga
     9601 ccagagcgaa caggttccgg gcatgatcga gcgctacact gctgccatca ctggtgcaga
     9661 aggcaagatc caccgtctgg aagactgggg ccgccgtcag ctggcttacc cgatcaacaa
     9721 actgcacaaa gcacactacg ttctgatgaa cgttgaagct ccgcaggaag tgatcgatga
     9781 gctggaaact accttccgct tcaacgatgc cgttatccgc agcatggtta tgcgtaccaa
     9841 gcacgctgtt accgaagcat ctccgatggt taaagcgaaa gacgagcgcc gtgagcgtcg
     9901 cgatgatttc gcaaacgaaa ccgctgatga tgctgaagct ggggattctg aagagtaatt
     9961 tctgatgacc aaccgtctgg tgttgtccgg caccgtgtgc aggactcccc ttcgaaaggt
    10021 cagtccatca ggaattcctc actgccagtt cgtgcttgag catcgttctg tgcaggagga
    10081 agccggcttt caccggcagg catggtgtca aatgcccgtt attgttagcg gacacgaaaa
    10141 ccaggccatt actcacagta taacggtcgg cagtcgcata accgttcagg ggttcatttc
    10201 atgccacaag gcaaagaacg gactgagcaa aatggttttg catgccgagc agattgaatt
    10261 gatagattct ggagactagc catatggcac gttatttccg tcgtcgcaag ttctgccgtt
    10321 tcaccgcgga aggcgttcaa gagatcgact ataaagatat cgctacgctg aaaaactaca
    10381 tcaccgaaag cggtaagatt gtcccaagcc gtatcaccgg tacccgtgca aaataccagc
    10441 gtcagctggc tcgcgctatc aaacgcgctc gctacctgtc cctgctgccg tacactgatc
    10501 gccatcagta atcggtcacg gtccattaat acgactttga gaggataagg taatgcaagt
    10561 tattctgctt gataaagtag caaacctggg tagcctgggt gatcaggtaa acgttaaagc
    10621 gggctatgct cgtaacttcc tggtaccgca gggtaaagct gttccagcta ccaagaaaaa
    10681 cattgaattc ttcgaagcac gtcgcgctga actggaagct aaactggctg aagttctggc
    10741 agctgctaat gctcgcgctg agaaaatcaa tgcactggaa actgttacca tcgcgtctaa
    10801 agctggcgac gaaggtaaac tgttcggttc catcggtact cgcgacatcg ctgacgctgt
    10861 aactgcagct ggcgttgaag tggctaagag cgaagttcgt ctgccgaacg gcgttctgcg
    10921 taccactggc gaacacgaag tgagcttcca ggttcacagc gaagtattcg cgaaagtgat
    10981 cgtaaacgta gtagctgaat aattcgttat tcaacgagac gtaaaaacgc cgaccattgg
    11041 tcggcgtttt gctttctatt tttcgtcagg cattaatttc gcaagtagat ccattcacca
    11101 ttctcttgtg tccataagtc aggatggttt gctggcgtta gatgcatacg atcactcctt
    11161 atgtctcggt cctgcggcac tcgctcgaaa cctaaacccg agtagattgc ggctgaattt
    11221 gataaggaac gtagaccaat tttcaaatca tggggtaaat cagaatacgc gtttagcaat
    11281 gcagcaatga tgaatccgag ggcattagaa acgcctgtaa cagtgtgaag ttggtcgata
    11341 taaagatcga ttgttggtag ttcagatgag cagcaattag gcatttgggc aggaagggtt
    11401 acaacgtatc ctatcgtttg gccggtatag tctggagtac tataaccagg tcccaaaaca
    11461 acctcgtgta ttgtgtattt actgttgggt tcgaaggatt cgacatcttg caataaatat
    11521 ttcagttctg cggttaacct cgcacccgcg taagctttat tgtatttctg ggcgtgaata
    11581 ttatctaatg tagcttcatg gttccatcca aaagaattat caccattcat tgttactgaa
    11641 tcgccacggg tttaacagac acctcagagt catttaagat gg
//
LOCUS       DAJTRC010000109        11646 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.109, whole genome shotgun sequence.
ACCESSION   DAJTRC010000109 DAJTRC010000000
VERSION     DAJTRC010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11646)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11646)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11646
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.109"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            598..795
                     /locus_tag="OO357_003322"
     CDS             598..795
                     /locus_tag="OO357_003322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888161.1"
                     /translation="MEITPQQLVEFTIPDGRGQQARTLPDMLSSDAGPLSYPLIYSSK
                     VMIISLFDSTVMPDKYRHNGK"
     gene            complement(745..1938)
                     /locus_tag="OO357_003323"
     CDS             complement(745..1938)
                     /locus_tag="OO357_003323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5888162.1"
                     /translation="MSPGIEDTPQKPLSCWPLAFSAGLLGIGQNGLLVVLPVLVIQTN
                     LSLSVWAALLMLGSMLFLPSSPWWGKQISRTGSKPVVLWALGGYGISFTLLGLGSVLM
                     ATSAITTAVGLGILIIARIAYGLTVSAMVPACQVWALQRAGEGNRMAALATISSGLSC
                     GRLFGPLCAAAMLAIHPLAPLGLLMAAPVLALLMLLRLPGTPPQPTPECKSVSLKRDC
                     LPYLLCAILLAAAVSMMQLGLSPALTRQFATDTTAISQQVAWLLGLSAVAALIAQFGV
                     LRPQRLTPVALLLSAGVLMSGGLAIMLSEQLWLFYPGCAVLSFGAALATPAYQLLLND
                     KLADGAGAGWLATSHTLGYGLCALLVPLVSKTGVAIALIMAALFATILFTIVSVFIWH
                     YRTIK"
     gene            2074..3798
                     /gene="iucA"
                     /locus_tag="OO357_003324"
     CDS             2074..3798
                     /gene="iucA"
                     /locus_tag="OO357_003324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001314127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucA"
                     /protein_id="HCR5888163.1"
                     /translation="MTLPSEKPATDVAAQCFLNALIRETTDWKLTEYPPDELLIPLDE
                     QKSLHFRVAYFSPTQHHRFAFPARLVTASGSYPVDFTTLSRLIIDKLRHQLFLPVPLC
                     ETFHQRVLESHVHTQQAIDARHDWAALREKALNFGEAEQALLTGHAFHPAPKSHEPFN
                     RREAERYLPDMAPHFPLRWFSVDKTQIAGESLHLNLQQRLTRFAAENAPQLLNELSDN
                     QWLFPLHPWQGEYLLQQGWCQALVAKGLIKDLGEAGTSWLPTTSSRSLYCATSRDMIK
                     FSLSVRLTNSIRTLSVKEVKRGMRLARLAQTDGWQMLQVRFPTFRVMQEDGWAGLLDL
                     NGNIMQESLFALRENLLVDQPKSQTNVLVSLTQAAPDGGDSLLVSAVKRLSDRLGITV
                     QQAAHAWVDAYCQQVLKPLFTAEADYGLVLLAHQQNILVQMLGDLPVGFIYRDCQGSA
                     FMPHATDWLDSIGEAQAENIFTHEQLLRYFPYYLLVNSTFAVTAALGAAGLDSESNLM
                     ARVRASLAEVRDQVTHKTCLNYVLESPYWNVKGNFFCYLNDHNENTIVDPSVIYFDFA
                     NPLQAQEV"
     gene            3799..4746
                     /gene="iucB"
                     /locus_tag="OO357_003325"
     CDS             3799..4746
                     /gene="iucB"
                     /locus_tag="OO357_003325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N(6)-hydroxylysine O-acetyltransferase"
                     /protein_id="HCR5888164.1"
                     /translation="MSEANIIHSRYGLRCEKLDKPLNLGWGLDNSAVLHCPGELPTGW
                     LCDALDQIFIAAPQLSAVALPWAEWREEPQALTLFGQVKSDIIHRTAFWQLPLWLSSP
                     ANRASGEMVFDAEREIYFPQRPPRPQGEVYRRYDPRIRRMLSFRIADPVSDAERFTRW
                     MNDPRVEYFWEQSGSLEVQTAYLERQLTGKHAFPLIGCFDDRPFSYFEIYWAAEDRIG
                     RHYSWQPFDRGLHLLVGEQQWRGAHYVQSWLRGLTHYLLLDEPRTQRTVLEPRTDNQR
                     LFRHLEPAGYRTIKEFDFPHKRSRMVMADRHHFFTEVGL"
     gene            4746..6488
                     /gene="iucC"
                     /locus_tag="OO357_003326"
     CDS             4746..6488
                     /gene="iucC"
                     /locus_tag="OO357_003326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aerobactin synthase IucC"
                     /protein_id="HCR5888165.1"
                     /translation="MNHKDWDFVNRRLVAKMLSEMEYEQVFHAESQGDDHYCINLPGA
                     QWRFIAERGIWGWLWIDAQTLRCTDEPVLAQTLLMQLKPVLSMSDATVAEHMQDLYAT
                     LLGDLQLLKARRGLSASDLINLDADRLQCLLSGHPKFVFNKGRRGWGKEALERYAPEY
                     TNTFRLHWLAVKREHMIWRCDNDLDIQQLLTAAMDPQEFTRFSQVWQENGLDHNWLPL
                     PVHPWQWQQKIATDFIADFAEGRMVSLGEFGDQWLAQQSLRTLTNASRRGGLDIKLPL
                     TIYNTSCYRGIPGRYIAAGPLASRWLQQVFATDATLVQSGAVILGEPAAGYVSHEGYA
                     ALARAPYRYQEMLGVIWRENPCRWLKPDESPVLMATLMECDENNQPLAGAYIDRSGLD
                     AETWLTQLFRVVVVPLYHLLCRYGVALIAHGQNITLAMKEGVPQRVLLKDFQGDMRLV
                     KEAFPEMDSLPQEVRDVTSRLSADYLIHDLQTGHFVTVLRFISPLMVRLGVPERRFYQ
                     LLAAVLSDYMNKHPQMAERFALFSLFRPQIIRVVLNPVKLTWPDLDGGSRMLPNYLEN
                     LQNPLWLVTQEYES"
     gene            6485..7822
                     /gene="iucD"
                     /locus_tag="OO357_003327"
     CDS             6485..7822
                     /gene="iucD"
                     /locus_tag="OO357_003327"
                     /EC_number="1.14.13.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent L-lysine N(6)-monooxygenase"
                     /protein_id="HCR5888166.1"
                     /translation="MKKSVDFIGVGTGPFNLSIAALSHQIEELNCLFFDEHPHFSWHP
                     GMLVPDCHMQTVFLKDLVSAVAPTNPYSFVNYLVKHKKFYRFLTSRLRTVSREEFSDY
                     LRWAAEDMNNLYFSHTVENIDFDKKSRLFLVQTSRGEYFAHNICLGTGKQPYLPPCVK
                     HVTQSCFHASEMNLRRPDLSGKRITVVGGGQSGADLFLNALRGEWGEAAEINWVSRRN
                     NFNALDEAAFADEYFTPEYISGFSGLKEDIRHQLLDEQKMTSDGITADSLLTIYRELY
                     HRFEVLRKPRNIRLLPSRSVTTLESSGPGWRLLMEHHLDQGRESLESDVVIFATGYRS
                     ALPQILPSLMPLITMHDKNTFKVRDDFTLEWSGPKENNIFAVNASMQTHGIAEPQLSL
                     MAWRSARILNRVLGRDLFDLSMPPALIQWRSGSRKKPQPEAASLTHYTANIQE"
     gene            7828..10023
                     /gene="iutA"
                     /locus_tag="OO357_003328"
     CDS             7828..10023
                     /gene="iutA"
                     /locus_tag="OO357_003328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709458.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric aerobactin receptor IutA"
                     /protein_id="HCR5888167.1"
                     /translation="MRKKYMPRALGPLLLVVLSPAVAQQNDDNEIIVSASRSNRTVAE
                     MAQTTWVIENAELEQQIQGGKELKDALAQLIPGLDVSSQSRTNYGMNMRGRPLVVLID
                     GVRLNSSRSDSRQLDSVDPFNIDHIEVISGATALYGGGSTGGLINIVTKKGQPETMME
                     FEAGTKSGFNSSKDHDERIAGAVSGGNDHISGRLSVAYQKFGGWFDGNGDATLLDNTQ
                     TGLQHSNRLDIMGTGTLNIDESRQLQLITQYYKSQGDDNYGLNLGKGFSAISGSSTPY
                     VSKGLNSDRIPGTERHLISLQYSDSDFLGQELVGQVYYRDESLRYYPFPTVNANKQAT
                     AFSSSQQDTDQYGMKLTLNSQLMDGWQITWGLDAEHERFTSNQMFFDLAQASASGGLN
                     NHKIYTTGRYPSYDITNLAAFLQSSYDINDIFTVSGGVRYQYTENRVDDFIDYTQQQK
                     IATGKAISADAIPGGSVDYDNFLFNAGLLMHITERQQAWFNFSQGVALPDPGKYYGRG
                     IYGAAVNGHLPLTKSVNVSDSKLEGVKVDSYELGWRFIGDNLRTQIAAYYSLSNKSVE
                     RNKDLTISVKDDRRRIYGVEGAVDYLIPDTDWSTGVNFNVLKTESKVNGQWQKYDVKE
                     SSPSKATAYINWAPEPWSLRVQSTTSFDVSDAEGNDINGYTTVDFISSWQLPVGTLSF
                     SVENLFDRDYTTVWGQRAPLYYSPGYGPASLYDYKGRGRTFGLNYSVLF"
     gene            complement(10574..10717)
                     /locus_tag="OO357_003329"
     CDS             complement(10574..10717)
                     /locus_tag="OO357_003329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888168.1"
                     /translation="MEHRSVIHDELAIDHDAILQCAEEVLHHCVIQTVSFVRHILCYS
                     MGL"
     gene            10956..>11646
                     /locus_tag="OO357_003330"
     CDS             10956..>11646
                     /locus_tag="OO357_003330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5888169.1"
                     /translation="MNKIYSLKYSAATGGLIAVSELAKRVSGKTNRKLVATMLSLAVA
                     GTVNAANIDISNVWARDYLDLAQNKGIFQPGATDVTITLKNGDKFSFHNLSIPDFSGA
                     AASGAATAIGGSYSVTVAHNKKNPQAAETQVYAQSSYKVVDRRNSNDFEIQRLNKFVV
                     ETVGATPAETNPTTYSDALERYGIVTSDGSKKIIGFRAGSGGTSFINGESKISTNSAY
                     SHDLLSASLFEV"
BASE COUNT         2953 a         2894 c         2989 g         2810 t
ORIGIN      
        1 tggagtgaaa caaaggcagc ttatcgttgt ttttcaaacg ataaagtctc tgctaataaa
       61 atcatgactc ctcacaaaga aaatatcact gaaagagctc agcagtttaa aagagtttta
      121 gttttacagg atacaacaga acttaattat tctggtcaga aagaaaaaca aggagttggt
      181 cccaaaaaac ataaagatga aagaaactta tttcttcatc ctcaactcgt catcagtgag
      241 tcaggtgttt gcctcggtgt ttatgatgac tatcagtggt tcagggatga attaaaaacc
      301 agtaaaaata cccgacaaga aatctgcaat gatttacttc ataaaaaaca tgtcagtgaa
      361 aaagaaacct ggcgttgggt tgaaggatat aataaagcaa cagaactggc tcgccaatgc
      421 cctgataccc atgtattgtc aatttctgat cgtgaaggcg atttttatga cctgtttgaa
      481 cgcgcagcgc agactcccgg aataaaagct gactggcttg tcagaatgaa atttaataac
      541 agagcaacgc tgaatattaa tggtaaacgg gatcataggt tgttgcacga aagaatcatg
      601 gagattacac cgcagcaact ggttgaattc actatacctg atgggcgggg gcagcaagcc
      661 agaacactgc ctgacatgct ttcatcagac gcaggtcctc tctcatatcc tctgatttac
      721 tccagtaagg tgatgatcat atctttattt gatagtacgg taatgccaga taaatacaga
      781 cacaatggta aataatatag ttgcaaataa tgctgccata atcagcgcga ttgcgacacc
      841 tgttttcgat accagcggca ccagcaaagc gcacagtcca tagccaagtg tgtgactggt
      901 agcgagccag cccgcgcctg cgccatcagc cagcttatca ttcagtagaa gttgataagc
      961 gggtgttgcc agagctgctc caaatgacag cactgcacag cccgggtaaa acaaccatag
     1021 ctgttcggag agcatgatag ccagaccacc actcatcaac actccggcac tcaggagcag
     1081 ggccaccgga gtcaggcgct gcggacggag taccccgaac tgcgcgataa gcgcagctac
     1141 tgcggacaga cccaacagcc aggccacctg ttggctaatg gcggtggtat ccgtagcgaa
     1201 ctggcgagta agggcaggcg aaagtccaag ctgcatcatg ctcaccgccg cagccagtaa
     1261 tattgcgcaa agcagataag gcagacaatc ccgcttcaga ctgacgctct tgcactccgg
     1321 tgtgggctgt ggtggtgtgc cgggcaaccg cagcagcatc agcagcgcca gcactggggc
     1381 tgccatcagc agccccagtg gcgccagagg atgaatggcc agcattgctg ccgcgcacag
     1441 cggcccgaat agcctgccgc aactcaggcc ggagctgatg gttgccagag cggccatgcg
     1501 attcccttct ccagctctct gcaatgccca gacctgacag gctggcacca ttgccgacac
     1561 ggtcagcccg taggcgatcc gggcgatgat taatattccc agtcccaccg ctgttgttat
     1621 ggcgctggta gccatcagca cgcttcccag ccctagcagg gtaaagctta tgccatatcc
     1681 tcccagagcc cacaacacca caggtttact gccagtacgg gaaatttgct ttccccacca
     1741 cggggacgat ggcagaaaca gcattgagcc aagcatcagc agggcagccc atacagacag
     1801 actcagattt gtctgtatga ccagaacagg gagcacaacc agcagaccgt tctgtccgat
     1861 accgagaagc ccggcactga acgcaagtgg ccagcaggac agtggttttt ggggcgtatc
     1921 ttcgatccct ggtgacatta taatgtttca actccatgta ttaattgtgt ttatttgtaa
     1981 aattaattta tctgacaata acatttattg ttgataatga gaatcattat tgacataatt
     2041 gttattattt tactgtgtgg gagctgtttg actatgaccc tgccctctga aaaaccagcc
     2101 acagatgtgg ctgcgcagtg cttcctgaat gcactgattc gtgaaaccac agactggaaa
     2161 ctgacagaat acccgccaga cgaattgctc atcccgctgg atgagcagaa atcgctccat
     2221 ttcagagtgg cttatttctc cccaacccag catcaccgct ttgcatttcc tgcacgtctg
     2281 gtcacggcat caggcagtta tcctgtcgac tttaccactc tctcccggct tattattgat
     2341 aagctacgcc atcaactgtt tctgcccgtt cccctctgcg aaactttcca ccagcgcgtg
     2401 ctggaaagcc atgtccatac gcaacaggca attgatgccc gtcatgactg ggccgctctg
     2461 cgtgaaaaag cgttgaattt tggcgaggct gagcaggcgc tgctgacagg acacgctttc
     2521 caccctgcgc ctaagtctca tgaaccgttt aaccggcggg aggctgaacg ctacctgcct
     2581 gatatggcac cccacttccc actgcggtgg ttttcggtgg ataaaacgca aatcgccggt
     2641 gaaagtttgc atctcaacct tcaacagcgg ctgacgcgat ttgccgcaga gaatgcgcct
     2701 cagttactca acgaattaag tgacaaccaa tggctgttcc cgttgcaccc gtggcagggg
     2761 gaatatcttt tgcagcaggg gtggtgccag gcacttgttg ctaaagggct gattaaagac
     2821 ttaggtgagg ccggcacgtc gtggctgccg accacctctt cccgttccct ctactgtgcc
     2881 accagccgcg atatgatcaa gttctccctg agcgtacggc tgaccaactc catccgtact
     2941 ctgtccgtga aagaagtgaa gcgtgggatg cgcctggcac gcctggctca aaccgacggc
     3001 tggcagatgc tacaggtccg cttcccgact ttccgggtaa tgcaggagga tggctgggcc
     3061 gggctgctcg atcttaacgg caacatcatg caggaaagtc tgtttgccct gcgtgaaaat
     3121 ctgctggtgg atcagccgaa aagccagact aacgtactgg tctccctgac tcaggccgca
     3181 cctgatggcg gtgattcgct gctggtttcg gcggtaaaac gcctgagcga tcgcctcggt
     3241 atcactgtgc aacaggccgc ccatgcatgg gttgatgcat actgtcagca agtgctaaag
     3301 ccgctgttta cggctgaagc ggattacggc ctggtgctgc tggcgcatca gcaaaatatt
     3361 cttgtccaga tgcttgggga tctgccggtc ggatttattt accgtgactg tcagggcagc
     3421 gcttttatgc ctcacgcgac agactggctc gattctattg gcgaggcgca ggcggaaaat
     3481 attttcaccc atgagcagtt gctgcgctat ttcccttatt acctgctggt taactccact
     3541 tttgctgtga ccgctgcgct gggggctgcc gggctggaca gcgaatcgaa tctgatggct
     3601 cgtgtacgag catcgctggc tgaagtgcgt gatcaggtga ctcataaaac ctgtctcaac
     3661 tacgtgctgg aaagtccgta ctggaacgta aaaggtaact ttttctgtta tctgaacgat
     3721 cataacgaga acaccattgt tgacccttcg gtgatctatt tcgatttcgc taacccgctg
     3781 caggctcagg aggtctgaat gtctgaggca aacattattc acagcagata tggactgcgc
     3841 tgtgaaaaac tcgacaagcc cctgaatctt ggctggggac tggacaatag cgcggtgttg
     3901 cactgtcccg gggagctgcc gacagggtgg ctgtgcgatg cgctggatca gatatttatc
     3961 gccgcaccac aactgtcagc agtggctctc ccctgggccg aatggcgtga ggagccacag
     4021 gcgctgacgc tttttgggca ggtaaaaagc gacattatcc atcgtactgc cttctggcag
     4081 ttaccgttat ggttgagttc accggcaaac cgggcctccg gtgaaatggt ttttgatgca
     4141 gagcgtgaga tttatttccc gcagcgccca ccccgtccac agggtgaggt ttaccgccgt
     4201 tacgatccac gtattcgcag gatgctgagt ttccgcattg ccgatcccgt ttctgatgca
     4261 gaacgtttca cccgctggat gaatgatccg cgcgttgagt atttctggga gcaaagtggc
     4321 tcactggagg tacagaccgc ctatctggag cgccagctca ccggtaaaca tgcattcccg
     4381 ctgattgggt gcttcgacga tcggccattc agctatttcg aaatctactg ggcggcggaa
     4441 gaccgcattg gtcgtcacta ttcgtggcag ccctttgacc gtggcctgca tctgctggtt
     4501 ggtgaacagc aatggcgcgg ggcccactat gtgcaaagct ggctgcgcgg gctgacacat
     4561 tacctgctgc tggatgagcc ccgtacgcag cgcacagtac tggagccacg caccgataac
     4621 cagcgcctgt tccgccatct tgagcctgcg ggataccgga caattaaaga gttcgacttc
     4681 ccacataagc gctcgcgcat ggtgatggcg gatcgtcatc acttcttcac ggaggtcggt
     4741 ctgtaatgaa tcacaaggac tgggattttg ttaaccgtcg gctggtggca aaaatgttgt
     4801 ctgagatgga gtatgagcag gttttccacg cggaatctca gggcgatgac cactactgca
     4861 ttaacctgcc gggagcacaa tggcgcttca tcgctgaacg tggtatctgg ggctggctgt
     4921 ggattgatgc ccaaacgttg cgctgcacgg acgaaccagt actggcgcag acgctgctga
     4981 tgcagctaaa gccggtgctg tcaatgagtg atgccactgt tgcagagcat atgcaggatt
     5041 tgtatgccac gctactgggc gacctgcaac tgctgaaggc ccgtcgcgga ctgagtgcca
     5101 gtgacctgat taatcttgat gccgatcgtc tgcaatgcct gctgagcggt catcctaaat
     5161 tcgtttttaa taaaggtcgc cgtggctggg gcaaagaggc gctggaacga tatgcgccag
     5221 agtataccaa caccttcaga ctgcactggc tggcggtaaa acgtgaacat atgatctggc
     5281 gctgcgataa cgatctggat attcagcagt tgttgactgc cgcaatggat ccgcaggagt
     5341 ttacacgctt cagtcaggtc tggcaggaaa acgggctgga tcacaactgg ctgccgctgc
     5401 cggtacatcc gtggcagtgg cagcaaaaaa tcgccacaga cttcatcgct gattttgccg
     5461 aaggcaggat ggtgtctctc ggcgagtttg gcgaccagtg gctcgcccag cagtcgctgc
     5521 gtaccctgac caacgccagc cgccggggag ggctggatat caagctgccg ctgaccatct
     5581 acaatacctc atgctaccgg gggatccctg gcagatatat cgctgccgga ccactggctt
     5641 cacgctggct gcaacaggtt tttgcgaccg acgccaccct ggtgcaaagc ggcgcagtga
     5701 tccttggtga accggctgca ggctatgtgt cccatgaagg ctatgccgcg cttgcccggg
     5761 ctccctatcg ctaccaggaa atgcttggtg ttatctggcg ggagaatccg tgccgctggc
     5821 tgaaaccgga tgaaagtcct gttctgatgg caacactgat ggagtgcgac gaaaacaatc
     5881 agccgctggc aggcgcatat atcgaccgct ccggactgga cgctgaaacc tggcttacgc
     5941 aattgttccg ggtggttgtg gttccgctgt atcacctgct ttgccgctac ggtgtcgcgc
     6001 ttattgcaca cggacaaaat ataacgctcg ccatgaaaga gggggtacca cagcgtgttc
     6061 tgctgaaaga cttccagggc gatatgcggc tggtgaaaga agcgtttccc gaaatggact
     6121 ctttgcctca ggaggtgcgt gatgtcacat cccgtctgag tgcggactac ttaatccatg
     6181 atttgcagac gggtcacttc gtgacagtac tgcgttttat ttcgccactg atggttcgtc
     6241 ttggcgtacc tgaaaggcga ttttatcaac tgctggcagc agtgttgagt gattacatga
     6301 acaaacatcc acaaatggca gagcgttttg cgcttttctc actcttcagg ccacaaatca
     6361 ttcgcgtggt gctgaacccg gtaaaactga cctggccgga cctggatggc ggcagtcgca
     6421 tgctgccgaa ttaccttgag aatctgcaaa atccgctgtg gctggtaact caggaatatg
     6481 aatcatgaaa aaaagtgtcg attttattgg tgtcgggaca gggccattta atctcagcat
     6541 tgctgcattg tcacatcaga tcgaagaact gaactgtctc ttctttgacg aacatcctca
     6601 tttttcctgg catccgggta tgctggtacc ggattgtcat atgcagaccg tctttctgaa
     6661 agatctggtc agtgcagttg cacctacaaa tccctacagc tttgttaact atctggtgaa
     6721 gcacaaaaag ttctatcgct tccttacaag caggctacgt acagtatccc gtgaagagtt
     6781 ttctgactat ctccgctggg ctgctgaaga tatgaataac ctgtatttca gtcataccgt
     6841 tgaaaatatt gatttcgaca aaaaaagtcg attgtttctg gttcagacca gtcggggaga
     6901 atattttgcc cacaatatct gccttggtac aggaaaacaa ccttatttac caccctgtgt
     6961 gaagcatgtg acacaatcct gtttccatgc cagtgaaatg aatcttcgtc ggccggacct
     7021 gagtggaaaa cggataaccg tggttggtgg aggacagagt ggtgcagacc tgttccttaa
     7081 tgcattacgc ggggaatggg gagaagcggc ggaaataaac tgggtctcac ggcgtaataa
     7141 ttttaacgca ctggatgagg ctgcttttgc tgatgagtat tttacacctg aatatatttc
     7201 aggcttctcc ggactgaagg aagatattcg ccatcagtta ctggatgagc agaaaatgac
     7261 atcggatggc atcactgccg attctttact gaccatttat cgtgagttgt accaccgttt
     7321 tgaagttctg agaaaaccaa gaaatatccg tctgctaccc agtcgctcgg taacaactct
     7381 ggaaagtagt ggtccgggct ggaggttatt gatggaacat catctggatc agggcagaga
     7441 gagcctggaa agtgatgtgg tgattttcgc cacaggttac cgttctgcat tgccacaaat
     7501 acttccctca ctgatgccct tgatcaccat gcacgataag aacaccttta aagtgcgtga
     7561 tgacttcact ctggaatgga gtggcccgaa agaaaataac atctttgcgg tcaacgccag
     7621 catgcaaact catggcatcg ccgaacccca gctcagcctg atggcctggc gatctgcacg
     7681 tattcttaat cgcgtactgg ggcgtgattt attcgatctc agtatgccgc ccgcactgat
     7741 tcagtggcgc agcggcagcc ggaaaaaacc acagccggag gctgcttctt taactcacta
     7801 tacagcaaat attcaggaat aacgatgatg cgcaaaaagt atatgccccg ggctcttggt
     7861 ccgctgcttc ttgtcgtgct gtcaccagct gtcgcccagc aaaacgatga taatgagatc
     7921 atagtgtctg ccagccgcag caatcgaact gtagcggaga tggcgcaaac cacctgggtt
     7981 atcgaaaatg ccgaactgga gcagcagatt cagggcggta aagagctgaa agacgcactg
     8041 gctcagttaa tccccggcct tgatgtcagc agccagagtc gaaccaacta cggtatgaac
     8101 atgcgtggcc gcccgctggt tgtcctgatt gacggtgtgc gcctcaactc ttcacgttcc
     8161 gacagccgac aactggactc tgtcgatcct tttaatatcg accatattga agtgatctcc
     8221 ggcgcgacgg ccctgtacgg tggcgggagt accggagggt tgatcaacat cgtgaccaaa
     8281 aaaggtcagc cggaaaccat gatggagttt gaggctggca caaaaagtgg ctttaacagc
     8341 agtaaagatc acgatgagcg cattgccggt gctgtctccg gcggaaatga ccatatctcc
     8401 ggacgtcttt ccgtggcata tcagaaattt ggcggctggt ttgacggtaa cggcgatgcc
     8461 accctgcttg ataacaccca gaccggcctg cagcactcca atcggctgga catcatggga
     8521 accggtacgc tgaacatcga tgaatcccgg cagcttcaac tgataacgca gtactataaa
     8581 agtcagggag acgacaatta cgggcttaat ctcgggaaag gcttttccgc catcagcggg
     8641 agcagcacac catacgtcag taaggggctg aattctgacc gcattcccgg cactgagcgg
     8701 catttgatca gcctgcagta ctctgacagt gatttcctgg gacaggaact ggtcggtcag
     8761 gtttactacc gcgatgagtc gttgcggtac tacccgttcc cgacggtaaa tgcgaataaa
     8821 caggcgacgg ctttctcctc gtcacagcag gataccgacc agtacggcat gaaactgact
     8881 ctgaacagcc aactgatgga cggctggcaa atcacctggg ggctggatgc tgagcatgag
     8941 cgctttacct ccaaccagat gttcttcgat ctggctcagg caagtgcttc cggagggctg
     9001 aacaaccata agatttacac caccgggcgc tatccgtcat atgacatcac caatctggcg
     9061 gccttcctgc aatccagcta tgacattaat gatattttta ccgttagcgg tggcgtacgc
     9121 tatcagtata ctgagaacag ggtagatgat ttcatcgact acacgcagca acagaagatt
     9181 gctaccggga aggcgatatc tgccgacgcc attcctggtg gttcggtaga ttacgataac
     9241 tttctgttca atgctggtct gctgatgcac atcaccgaac gtcagcaggc atggttcaat
     9301 ttttcccagg gggtggcatt gccggatccg gggaaatatt atggtcgcgg catctatggt
     9361 gcagcagtga acggccatct tcccctgaca aagagcgtga acgtcagcga cagtaagctg
     9421 gaaggcgtga aagtcgattc ttatgaactg ggctggcgct ttatcggtga caacctgcgg
     9481 actcaaatcg cggcatatta ctcgctttcc aataagagcg tggaaaggaa taaagatctg
     9541 accatcagtg tgaaggacga caggcgccgt atttacggcg tggaaggtgc ggtggactac
     9601 ctgatcccgg atactgactg gagtaccggt gtgaacttca atgtgctgaa aaccgagtcg
     9661 aaagtgaacg gtcaatggca aaaatatgac gtgaaggaat caagtccatc gaaagcgaca
     9721 gcttacatta actgggcgcc ggaaccgtgg agtctgcgtg tacagagcac cacttctttc
     9781 gacgtaagcg atgcagaggg taacgatatt aatggttaca ctaccgtcga ttttatcagt
     9841 agttggcagc ttccggtggg aacactcagc ttcagcgttg agaacctctt cgaccgtgac
     9901 tataccactg tctggggaca gcgtgcacct ctgtactaca gcccgggtta cggccctgct
     9961 tcactgtacg actacaaagg ccggggccga acctttggtc tgaactactc agtgctgttc
    10021 tgacggggat gcttttgcaa caagggtatg ctgtaaacaa catggtcctt ggtggccatg
    10081 ttgtttacag ccagtataat atacagcctt aacaagatat tccgtctccg gaatgggctg
    10141 ttcaccactg aataacatta tgtctgtttt atggatcttt gactgaaatt tatcgcatat
    10201 cctcatttca ttgtccgtct gaaaactaac cttacaagta agcgtcgtct cagcaccgtc
    10261 tggcagatcc tgaaattcct gagaggatga tctgcgccca atatcctgag ccggaacttt
    10321 gctgcaatga aggccaacga aaaatggggg agtatgttat agagtacttg ctacagggta
    10381 aaaagaggta cctgtcgctg gttctccgtt cagtttaacc gggagatttt ctcctgcgcc
    10441 tgtcataaag tccggtaatg gagattgtta atacgatggt gcacgatgca tcgtcagtgc
    10501 ccggcccgga ggatgccccg tagctacata cggatcaggg ttggcaatat cgaatggcag
    10561 gctataggca aagttaaagc cccatggagt agcacaaaat atgccgcaca aaggaaacgg
    10621 tctgaataac gcagtgatgg agaacttctt cggcacactg caaaatcgca tcgtggtcga
    10681 tcgctaattc gtcgtgaatc actgaacgat gttccatgcg aacagatatt cgatcatatc
    10741 gaaatattaa acattacccc tccccacatt gggcaaaaaa aaacaaaaaa cgatcaatta
    10801 gtattgattc acataatctc tcatcattaa tgagaacatt gtcacatatt tacaatatag
    10861 aactgttact tttagcaagc tgttagtaac caaccgttac taataatttt aattcttaat
    10921 actttgttca ttcaataaat gagttgagag aatatatgaa taaaatatac tcccttaaat
    10981 atagtgctgc cactggcgga ctcattgctg tttctgaatt agcgaaaaga gtttctggta
    11041 aaacaaaccg aaaacttgta gcaacaatgt tgtctctggc tgttgcaggt acagtaaatg
    11101 cagcaaatat tgatatatca aatgtatggg cgagagacta tcttgatctt gcacaaaata
    11161 aaggtatttt ccagcccgga gcaacagacg taacaatcac tttaaaaaac ggagataaat
    11221 tctctttcca taatctctca attccggatt tttctggtgc agcagcgagt ggcgcagcta
    11281 ccgcaatagg aggttcttat agtgttactg ttgcacataa caaaaagaac cctcaggccg
    11341 cagaaactca ggtttacgct cagtcttctt acaaggttgt tgacagaaga aattccaatg
    11401 attttgagat tcagaggtta aataaatttg ttgtggaaac agtaggtgcc accccggcag
    11461 agaccaaccc tacaacatat tctgatgcat tagaacgcta cggtatagtc acttctgacg
    11521 gttcaaaaaa aatcataggt tttcgtgctg gctctggagg aacatcattt attaatggtg
    11581 aatccaaaat ctcaacaaat tcagcatata gccatgacct gttaagtgct agtctatttg
    11641 aggtca
//
LOCUS       DAJTRC010000110        11636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.110, whole genome shotgun sequence.
ACCESSION   DAJTRC010000110 DAJTRC010000000
VERSION     DAJTRC010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.110"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(1..634)
                     /locus_tag="OO357_003331"
                     /pseudo
     CDS             complement(1..634)
                     /locus_tag="OO357_003331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS911 family transposase"
     gene            864..1214
                     /locus_tag="OO357_003332"
     CDS             864..1214
                     /locus_tag="OO357_003332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000905996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HCR5888170.1"
                     /translation="MLILDSRPVHASRPHSEAIRDAQRKKPKVPVHAVLTATNPLIRF
                     IGSDDMTQNRELFQVWLQKLAQWHQTTTPYLFLHTPDIAQAPELVHTLWEDLRKTLPE
                     IGAVPAIPQQSSLF"
     gene            1267..1662
                     /locus_tag="OO357_003333"
     CDS             1267..1662
                     /locus_tag="OO357_003333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HCR5888171.1"
                     /translation="MVSALYAVLSALLLMKFSFDVVRLRMQYRVAYGDGGFSELQSAI
                     CIHGNAVEYIPIAIVLMLFMEMNGAETWMVHICGIVLLAGRLMHYYGFHHRLFRWRRS
                     GMSATWCALLLMVLANLWYMPWELVFSLR"
     gene            1703..2446
                     /gene="cmoA"
                     /locus_tag="OO357_003334"
     CDS             1703..2446
                     /gene="cmoA"
                     /locus_tag="OO357_003334"
                     /EC_number="2.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxy-S-adenosyl-L-methionine synthase CmoA"
                     /protein_id="HCR5888172.1"
                     /translation="MSHRDTLFSAPIARLGDWTFDERVAEVFPDMIQRSVPGYSNIIS
                     MIGMLAERFVQPGTQVYDLGCSLGAATLSVRRNIHHDNCKIIAIDNSPAMIERCRRHI
                     DAYKAPTPVDVIEGDIRDIAIENASMVVLNFTLQFLEPSERQALLDKIYQGLNPGGAL
                     VLSEKFSFEDAKVGELLFNMHHDFKRANGYSELEISQKRSMLENVMLTDSVETHKARL
                     HKAGFEHSELWFQCFNFGSLVALKAEDAA"
     gene            2443..3414
                     /gene="cmoB"
                     /locus_tag="OO357_003335"
     CDS             2443..3414
                     /gene="cmoB"
                     /locus_tag="OO357_003335"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 5-methoxyuridine(34)/uridine 5-oxyacetic
                     acid(34) synthase CmoB"
                     /protein_id="HCR5888173.1"
                     /translation="MIDFGNFYSLIAKNHLSHWLETLPAQIANWQREQQHGLFKQWSN
                     TVEFLPEIKPYRLDLLHSVTAESEEPLSTGQIKRIETLMRNLMPWRKGPFSLYGVNID
                     TEWRSDWKWDRVLPHLSDLTGRTILDVGCGSGYHMWRMIGAGAHLAVGIDPTQLFLCQ
                     FEAVRKLLGNDQRAHLLPLGIEQLPALKAFDTVFSMGVLYHRRSPLEHLWQLKDQLVN
                     EGELVLETLVIDGDENTVLVPGDRYAQMRNVYFIPSALALKNWLKKCGFVDIRIVDVC
                     VTTTEEQRRTEWMVTESLSDFLDPHDPSKTVEGYPAPKRAVLIARKP"
     gene            complement(3579..6008)
                     /gene="torZ"
                     /locus_tag="OO357_003336"
     CDS             complement(3579..6008)
                     /gene="torZ"
                     /locus_tag="OO357_003336"
                     /EC_number="1.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine N-oxide reductase TorZ"
                     /protein_id="HCR5888174.1"
                     /translation="MALTRREFIKHSGIAAGALVVTSAAPLPAWAEEKGGKILTAGRW
                     GAMSVEVKDGKIVSSTGALAKTIPNSLQSTAADQVHTTARIQHPMVRKSYLDNPLQPA
                     KGRGEDTYVQVSWEQALKLIHEQHERIRKANGPSAIFAGSYGWRSSGVLHKAQTLLQR
                     YMNLAGGYSGHSGDYSTGAAQVIMPHVVGSVEVYEQQASWPLILENSQVVVLWGMNSL
                     NTLKIAWSSTDEQGLEYFHQLKKSGKPVIAIDPIRSETIEFFGDNATWIAPNMGTDVA
                     LMLGIAHTLMTQGKHDKVFLEKYTTGYPQFEEYLTGKSDNTPKSAVWTAEITGVPEAQ
                     IVKLAELMAANRTMLMAGWGIQRQQYGEQKHWMLVTLAAMLGQIGTPGGGFGFSYHYS
                     NGGNPTRVGGVLPEMSAAIAGQASEAADDGGMTAIPVARIVDALENPGGKYQHNGKEQ
                     TYPNIKMIWWAGGGNFTHHQDTNRLIKAWQKPEMIVVSECYWTAAAKHADIVLPITTS
                     FERNDLTMTGDYSNQHIVPMKQAVAPQFEARNDFDVFADLAELLKPGGKEIYTEGKDE
                     MAWLKFFYDAAQKGARAQRVTMPMFNAFWQQNKLIEMRRSEKNEQYVRYGDFRADPVK
                     NALGTPSGKIEIYSKTLEKFGYKDCPAHPTWLAPDEWKGTADEKQLQLLTAHPAHRLH
                     SQLNYAELRKKYAVADREPITIHTEDAARFGIANGDLVRVWNKRGQILTGAVVTDGIK
                     KGVVCVHEGAWPDLENGLCKNGSANVLTADIPSSQRANACAGNSALVYIEKYTGNAPK
                     LTAFDKPAVQA"
     gene            complement(6033..7133)
                     /locus_tag="OO357_003337"
     CDS             complement(6033..7133)
                     /locus_tag="OO357_003337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707761.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NapC/NirT family cytochrome c"
                     /protein_id="HCR5888175.1"
                     /translation="MRGKKRIGLLFLLIAVVVGGGGLLLAQKALHKTSDTAFCLSCHS
                     MSKPFEEYQGTVHFSNQKGIRAECADCHIPKSGMDYLFAKLKASKDIYHEFVSGKIDS
                     DDKFETHRQEMAETVWKELKATDSATCRSCHSFDAMDIASQSESAQKMHNKAQKGGET
                     CIDCHKGIAHFPPEIKMDDNAAHELESQAATSVTNGAHIYPFKTSRIGELATVNPGTD
                     LTVVDASGKQPIVLLQGYQMQGSENTLYLAAGQRLALATLSEEGIKALTVNGEWQADE
                     YGNQWRQASLQGALTDPALADRKPLWQYAEKLDDTYCAGCHASIAADHYTVNAWPSIA
                     KGMGARTSMSENEPDILTRYFQYNAKDITEKQ"
     gene            complement(7521..8267)
                     /gene="cutC"
                     /locus_tag="OO357_003338"
     CDS             complement(7521..8267)
                     /gene="cutC"
                     /locus_tag="OO357_003338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HCR5888176.1"
                     /translation="MALLEICCYSMECALTAQQNGADRVELCAAPKEGGLTPSLGVLK
                     SVRQRVTIPVHPIIRPRGGDFCYSDGEFAAILEDVRTVRELGFPGLVTGVLDVDGNVD
                     MPRMEKIMAAAGPLAVTFHRAFDMCANPLYTLNNLAELGIARVLTSGQKSDALQGLSK
                     IMELIAHRDAPIIMAGAGVRAENLHHFLDAGVLEVHSSAGAWQASPMRYRNQGLSMSS
                     DEHADEYSRYIVDGAAVAEMKGIIERHQAK"
     gene            complement(8281..8847)
                     /locus_tag="OO357_003339"
     CDS             complement(8281..8847)
                     /locus_tag="OO357_003339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5888177.1"
                     /translation="MANWQSIDELQDIASDLPRFTHALDELSRRLGLDITPLTADHIS
                     LRCHQNVTAERWRRGFEQCGELLSEKMINGRPICLFKLHEPVQVAHWQFSNVELPWPG
                     EKRYPHEGWEHIEIVLPGDPETLNARALALLSDEGLSLPGISVKTSSPKGEHERLPNP
                     TLAVTDGKTTIKFHPWSIEEIVASEQSA"
     gene            9063..10796
                     /gene="argS"
                     /locus_tag="OO357_003340"
     CDS             9063..10796
                     /gene="argS"
                     /locus_tag="OO357_003340"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001025321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HCR5888178.1"
                     /translation="MNIQALLSEKVRQAMIAAGAPADCEPQIRQSAKVQFGDYQANGM
                     MAVAKKLGMAPRQLAEQVLTHLDLNGIASKVEIAGPGFINIFLDPAFLAEHVQQALAS
                     DRLGVATPEKQTIVVDYSAPNVAKEMHVGHLRSTIIGDTAVRTLEFLGHKVIRANHVG
                     DWGTQFGMLIAWLEKQQQENAGEMELADLEGFYRDAKKHYDEDEEFAERARNYVVKLQ
                     SGDEYFREMWRKLVDITMTQNQITYDRLNVTLTRDDVMGESLYNPMLPGIVADLKAKG
                     LAVESEGATVVFLDVFKNKEGEPMGVIIQKKDGGYLYTTTDIACAKYRYETLHADRVL
                     YYIDSRQHQHLMQAWAIVRKAGYVPESVPLEHHMFGMMLGKDGKPFKTRAGGTVKLAD
                     LLDEALERARRLVAEKNPDMPADELEKLANAVGIGAVKYADLSKNRTTDYIFDWDNML
                     AFEGNTAPYMQYAYTRVLSVFRKAEINEEQLAAAPVIIREDREAQLAARLLQFEETLT
                     VVAREGTPHVMCAYLYDLAGLFSGFYEHCPILSAENEEVRNSRLKLAQLTAKTLKLGL
                     DTLGIETVERM"
     gene            10973..11461
                     /locus_tag="OO357_003341"
     CDS             10973..11461
                     /locus_tag="OO357_003341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HCR5888179.1"
                     /translation="MFKFLVLTLGIISCQAYAEDTVIVNDHDTSAIKDCWQKNSDDDT
                     DINVIKSCLRQEYNLVDAQLNKAYGEAYRYIEQVPRTGAKKLDTEQLNLLKKSQRAWL
                     DFRDKECELILSNEDVQDLSDPYSESEWLSCMIIQTNTRTRQLQLYRNSEDFYPSPLT
                     RG"
BASE COUNT         2812 a         2970 c         2779 g         3075 t
ORIGIN      
        1 tcaaggtagt tagggatagc aacatgttca tgaccaccac gtttataccg gtgagtcggc
       61 tgctgacagc tgaccagccc cagctctttc atgagcctgc cagcaagcca gcgtcccatc
      121 tggtagcctc tccgggttgc cattgtggcg atgcttcttg ctccggccga accgtggctg
      181 atgccatgta gctcaagtac ctgactgcgt aatacagccc gtctgccgtc tggtttttca
      241 ggacggtttt tccagtatct gtagctgctg cgatgaaccc cgaacacatg gcagagtgtg
      301 accacaggat aatgcgctct gagtttcccg attatcgaga actgttcagg gagtctgaca
      361 tcaagagcgc ggtagccttt tttaatattt cattctccat ttcaatgcgt tgtagctttt
      421 tcctgagctc acggatttca atttgttccg gggtaatggg ggaggctttt ggtgttttgc
      481 cctgacgctc atcacgcagt tgtttgaccc atcttgtcat tgtggaaagg ccaacatcca
      541 tagctttggc ggcatctgcc accgtgtatt tctggtcaac aaccagttga gcggattcgc
      601 gtttaaactc tgcgctaaaa tttctttttt tcattggagc acctgtgttg ttctgaggtg
      661 agcatatcac ctctgttcag gtggccaaat tcagtgtgcc acttcaccca taatgggctt
      721 ttaatcctat cgtttattgc ttgcatagta gaatctaacc atccatcact catagctaac
      781 cctcggaaat cttacatttc tgtctatcat aaccactagc atcaccttta ggaaccatct
      841 gaaacgggag gtgtatttag accttgctaa tcttagacag ccgcccggtt catgcatcac
      901 gtccacacag tgaagctatt cgcgacgctc aacgaaaaaa acctaaagtt ccggtacatg
      961 ctgtactgac ggcgacaaat ccgctgatcc gttttatcgg tagtgatgat atgacgcaaa
     1021 accgggaatt atttcaggtc tggttacaaa aattagcgca gtggcatcag accactacgc
     1081 cttatctttt tttacatacg ccagatattg cccaggcccc ggaactggta cataccctgt
     1141 gggaagactt acgtaaaacg cttccagaga tcggagcagt tccggctatt ccacagcaat
     1201 cttctctttt ctgaatttgc cacctatcat agacaggtgc catcggccat tttaaaggga
     1261 gtttgtatgg taagcgcgct atatgccgtt ttaagtgcgt tgttattaat gaagttctct
     1321 tttgatgtcg ttcgcctgcg aatgcagtac cgcgttgcct atggtgacgg tggttttagc
     1381 gaactgcaaa gcgctatttg cattcatggt aacgcggtgg aatatattcc catcgcgatt
     1441 gtgctgatgc tgtttatgga aatgaatggc gcagaaacct ggatggtgca tatttgcggc
     1501 atcgttttgc ttgctggtcg tctgatgcat tattacggtt ttcatcaccg tctgtttcgc
     1561 tggcgacgtt ccggcatgag cgccacctgg tgtgcgctgt tgctgatggt gctggcgaat
     1621 ctttggtata tgccctggga gttggttttc tccctgcgtt agcgcacaat acgccacttt
     1681 ctttttcccg gatttttacg ttatgtctca ccgcgacacg ctattttctg cccctatcgc
     1741 cagactgggc gactggacct ttgatgaacg ggtcgctgaa gtcttcccgg atatgatcca
     1801 gcgttccgtt cccggctatt ccaatattat ttccatgatt ggtatgttag ccgagcgctt
     1861 cgttcaacct ggtacgcagg tttacgatct gggttgttct ctgggcgcgg cgacgctctc
     1921 ggtgcgtcgc aacattcatc atgataattg caaaattatt gccatcgaca actccccggc
     1981 gatgattgaa cgctgccgtc gtcatattga cgcctataaa gcccctacgc cagtagacgt
     2041 cattgaaggt gatattcgcg atatcgccat tgaaaacgca tcgatggtgg tgctgaattt
     2101 taccctgcaa ttcctggaac cttccgagcg ccaggcatta ctggataaaa tttatcaagg
     2161 actgaacccg ggcggcgcgc tggtgctttc ggaaaaattc agtttcgaag atgccaaagt
     2221 tggtgaactg ttgttcaaca tgcaccacga ctttaaacgt gccaacggtt acagcgaact
     2281 ggagatcagc cagaagcgca gcatgctgga aaacgtgatg ctgaccgatt ccgtggaaac
     2341 ccataaagca cgcctgcata aagccggttt tgagcatagc gagctgtggt tccagtgctt
     2401 taactttggt tcactggtgg cattaaaagc agaggacgct gcatgatcga ctttggtaac
     2461 ttttattctc tgattgccaa aaatcatctt tcacactggc tcgaaacgct gcccgcgcag
     2521 attgctaact ggcagcgcga gcagcagcac gggctgttta agcagtggtc caacacggtg
     2581 gaatttctgc ctgaaattaa accgtatcgt ctggatttat tgcatagcgt aaccgccgaa
     2641 agcgaagagc cactgagcac cgggcaaatt aaacgcattg aaacgcttat gcggaacctg
     2701 atgccatggc gcaaagggcc gttctcactg tatggcgtca acatcgatac cgaatggcgt
     2761 tccgactgga aatgggatcg tgttctgccc catctttctg atttaaccgg acgcaccatt
     2821 ctggatgtcg gctgcggcag cggttatcac atgtggcgca tgattggcgc aggcgcgcac
     2881 ctcgcggtgg ggatcgaccc tacgcaactg tttctgtgcc agtttgaagc ggtgcgtaaa
     2941 ctactgggta acgatcagcg cgcacatttg ttaccgttag gtattgaaca gcttccggca
     3001 ctgaaagcct ttgataccgt cttttcgatg ggcgtgcttt accatcgccg ttcgccgctg
     3061 gagcatctct ggcagttgaa agatcaactg gtgaatgagg gtgaactggt gctggaaacg
     3121 ctggttattg atggcgatga gaacacggtg ctggtaccag gcgatcgtta cgcgcaaatg
     3181 cgtaatgtct atttcatccc ttccgcgctg gcgctgaaaa actggctgaa gaagtgtggt
     3241 tttgtagata tccgcattgt ggatgtgtgc gttaccacta ctgaagagca gcgacgcact
     3301 gaatggatgg ttaccgaatc tctgtccgat ttcctcgacc cacatgatcc gagtaagaca
     3361 gttgaaggtt atcctgcgcc taaacgcgcg gtgctgattg cgcgcaagcc gtaaaggtct
     3421 ggtattactg ccggatgcgg cgtgaacgcc ttatccggcc tacaaaccct tgctaattca
     3481 atctattgca gggactatgt aggcctgata agcatagcgc atcaggcagt tttacgtttg
     3541 cataacctca gcgcccgttt ccgggcgcta ttcacttctt acgcctgaac agcaggctta
     3601 tcaaatgccg ttaacttcgg tgcgttaccc gtatattttt cgatatacac cagcgcagag
     3661 ttaccggcac aggcatttgc ccgctgcgag ctggggatat ccgccgttaa cacgttcgca
     3721 ctgccgtttt tacacaagcc gttttccaga tctggccatg caccttcatg cacgcatacc
     3781 acgccttttt tgatcccgtc agtcaccacc gcgcctgtca aaatctgacc acgtttgttc
     3841 cagacacgca ccagatcgcc attcgcaata ccaaatcgag cagcatcttc ggtgtgaata
     3901 gtgatcggtt cacgatcggc aaccgcatat tttttacgca gttccgcata gttaagctga
     3961 ctgtgtaaac ggtgtgccgg atgtgcggtc agaagctgca actgcttctc gtcggcggta
     4021 cccttccact catcaggcgc aagccaggtt gggtgtgccg ggcaatcctt atagccaaat
     4081 ttttccagcg ttttggagta aatctcaatt ttgccgcttg gcgtacccag cgcatttttc
     4141 accggatcgg cgcggaaatc accataacga acgtactgtt cgttcttctc gctgcggcgc
     4201 atttcgatca gtttattttg ctgccagaag gcattaaaca ttggcatagt gacgcgttgc
     4261 gcacgggccc ctttctgagc ggcatcatag aaaaatttca gccacgccat ttcatcttta
     4321 ccttcggtat agatctcttt tccgccaggt ttgagtaatt ccgcaagatc ggcaaacacg
     4381 tcaaaatcgt tacgcgcttc aaattgcgga gcaacagcct gcttcatcgg cacaatatgc
     4441 tggttgctgt aatcaccggt catcgtcaag tcattgcgct caaacgatgt ggtgatcggt
     4501 aatacgatat ctgcgtgttt ggctgctgcg gtccagtagc attcagaaac gacgatcatc
     4561 tccggtttct gccaggcttt gatcagacga ttagtatcct ggtgatgggt aaagttaccg
     4621 ccacccgccc accagatcat tttgatattc ggatacgttt gttctttacc gttatgctga
     4681 tatttgccgc caggattttc cagcgcatcg acgatacggg caacaggaat cgccgtcatt
     4741 ccgccatcat cagcggcctc gctggcctgt ccggcaatcg ccgcagacat ttccggcagc
     4801 acgccgccaa cacgcgtcgg gttgccacca ttggagtaat gataagagaa accaaaaccg
     4861 ccgcccggtg taccaatctg ccctaacatc gccgccagcg tcaccagcat ccagtgtttc
     4921 tgttcaccat attgctggcg ctgaattccc cagcctgcca tcagcattgt gcgattcgcc
     4981 gccatcagtt cagcgagttt gactatctgc gcttccggta cgccagtaat ttccgccgtc
     5041 cagaccgcgc tcttcggcgt attatcgctc ttacctgtca gatactcttc aaactgcgga
     5101 tacccggtag tgtatttttc aaggaacact ttatcgtgtt tgccttgtgt catcagggta
     5161 tgcgcaatcc ctaacatcag tgccacgtcg gtgcccatat tcggcgcgat ccaggtggca
     5221 ttatcgccaa agaattcgat ggtttcggag cggataggat caatggcaat cactggtttg
     5281 ccagattttt tcagctgatg gaagtattcc agcccttgct catcggtact gctccaggca
     5341 atttttaagg tattcagcga gttcattccc cacagcacca caacctggct gttttccaga
     5401 atcagcggcc aggaggcctg ctgttcatac acctctacag aaccgaccac atgcggcatg
     5461 atcacctgtg ctgccccggt tgaataatcg ccgctatgcc cggaataacc gcccgccagg
     5521 ttcatataac gttgcagtaa ggtttgcgct ttatgcaaca cgccagaaga gcgccagccg
     5581 taagatccgg caaaaatggc cgatggtccg ttagctttac gaatacgctc atgttgctca
     5641 tgaatcagtt ttaatgcctg ttcccagctc acctgtacat aggtatcttc gccacgacct
     5701 ttcgccggtt gcagtggatt gtcgagatag ctttttctca ccatcggatg ctgaatacgc
     5761 gccgtggtgt gtacctgatc cgccgccgta gactgtaagg agttcggtat ggttttcgcc
     5821 agcgcgcctg tcgaagaaac aatcttgccg tccttcactt ctacgctcat cgctccccaa
     5881 cgtcccgcgg tgaggatttt accgcccttc tcttctgccc atgcggggag cggtgctgcc
     5941 gatgtcacca ccagcgctcc agcggcaata ccgctgtgtt taataaattc acgtcttgtt
     6001 aatgccataa cttcctccct gatcaacgag gatcactgtt tctcggtaat atctttggcg
     6061 ttgtactgga aataccgcgt taaaatgtcc ggttcgtttt cgctcatgct ggttcgtgcc
     6121 cccattcctt tggcaatgga cggccacgca ttgacggtgt aatggtcggc ggcaatagag
     6181 gcatgacaac cagcgcaata ggtatcgtca agtttttcag cgtattgcca tagcggttta
     6241 cggtccgcta atgcgggatc ggtaagcgca ccctgtaaag acgcctgacg ccattgattg
     6301 ccgtattcgt cagcctgcca ttccccgttt accgtgagcg ccttgatacc ttcttcactt
     6361 aatgtggcta gcgccagccg ttgacctgcc gccaggtaga gcgtgttttc actgccctgc
     6421 atttgataac cctgcaacag aacgatcggc tgtttgccac tggcatcaac gacggtgaga
     6481 tcggtaccag gattcacggt agccagctcg cctatgcgag aagttttgaa aggataaata
     6541 tgtgcgccat tagtcactga agtagcggcc tgactttcca gctcatgcgc cgcgttgtca
     6601 tccattttta tttctggcgg aaaatgggca atgcctttat gacaatcgat acaggtttcg
     6661 ccgccctttt gtgctttgtt atgcattttc tgcgcagatt cactttgcga ggcaatatcc
     6721 atggcatcaa aagaatggca actacggcac gttgcagagt cagttgcttt taattctttc
     6781 catactgttt cggccatttc ctggcgatga gtttcgaact tatcgtcact gtctattttg
     6841 ccgctaacaa attcatgata aatatcttta gatgctttta atttagcaaa taaataatcc
     6901 atccctgact ttggaatatg gcaatcggca cattccgcac gtatcccttt ctggttcgaa
     6961 aagtggacag ttccctgata ttcctcaaaa ggtttactca tcgagtggca ggaaaggcaa
     7021 aatgctgtat ccgacgtttt atgtaaggct ttttgcgcca gcaataaccc gccgccacca
     7081 accacaaccg ctatcagcaa aaacaataac ccaatgcgtt ttttccctcg catagattcc
     7141 tctaaataaa ttccagaaca ccgaaatcat tgtataatta aatacacaac gaattaaccg
     7201 cttgcataac tcggcacaac actgcctgaa ataatcgata aagaatatga tttattacaa
     7261 tgtaatcatt aattgctaag gaataaccca gttgctattt agaatatata tttatcagtt
     7321 ttagcaattt aaatcccata atcaatgtga atataaaaaa tatttttagc acaacgctat
     7381 gcgcactgtc acccgctcaa cagatcacgg ttatcgttcg ttttttatac tgctcaggga
     7441 taaaaaaagg cccctgttga aattgcaggg gcctggtacg agcaagcatc atattgggcg
     7501 acatgatgca acggtaaaaa tcatttggcc tgatggcgtt caatgattcc tttcatttca
     7561 gcaaccgccg ccccgtctac gatataacgc gaatactcgt ccgcgtgttc atctgatgac
     7621 atggacaatc cttgattacg ataacgcatc ggtgaggctt gccacgctcc cgcggagcta
     7681 tggacttcca gcactccggc atcgaggaag tggtgcaggt tttctgcacg gactcctgct
     7741 ccagccataa tgattggagc atcacgatgg gcaataagtt ccataatttt tgataaacct
     7801 tgcagtgcgt ctgatttttg ccctgatgtc agtacccggg caatgcccaa ttccgcaaga
     7861 ttattgagtg tatataaagg gttagcgcac atatcgaagg cgcgatgaaa agtcactgcc
     7921 agcggaccgg cagcagccat tattttttcc attcgtggca tatcgacatt cccgtcaaca
     7981 tcgagaacgc ccgtcaccag tccaggaaaa cctaattcgc ggaccgtgcg cacatcctca
     8041 agaatggcgg caaactcacc gtcgctgtag caaaaatcac caccgcgtgg gcgaattatc
     8101 ggatgcacag ggatcgtcac ccgctggcgc acggatttca gtacccccaa cgacggcgtt
     8161 aagcccccct cttttggggc tgcgcataat tcaactctgt ctgcgccgtt ttgctgcgcc
     8221 gttagtgcac attccatgct gtaacagcaa atttccagta atgccatttt tgctccttaa
     8281 ttacgctgac tgctcgctgg caacgatctc ttcaatggac cacggatgaa acttaatagt
     8341 tgttttgcca tcggtgaccg ccagcgtcgg attcggtaat cgctcatgct caccttttgg
     8401 tgaactggtt ttaactgaga tccccggtag gctgagtcct tcatcagaaa gcaatgccag
     8461 cgcacgggca ttcagcgttt ctggatcacc cggcagaacg atttcaatat gttcccaacc
     8521 ttcgtgtggg taacgttttt ccccgggcca cggtagctcc acattggaaa actgccagtg
     8581 cgcaacctgt accggttcat gcaatttaaa cagacaaatc ggtctgccat tgatcatttt
     8641 ttctgacaaa agctcgccac actgttcaaa accgcgacgc cagcgctcag cggtgacgtt
     8701 ttgatggcaa cgcaaagaaa tgtgatcggc agtcagcgga gtgatatcca accccagacg
     8761 gcgggaaagt tcatctaatg cgtgggtaaa tcgcggtaaa tccgatgcaa tatcctgcag
     8821 ctcgtcgata gattgccagt tcgccataat cactcttcgt ctttcagtaa aagcgttaat
     8881 ttaccctgtt gccctgtgcc aaccaaccgc tgatttcacg ccgcttctga tgcaatagtg
     8941 aaaacggcaa tacgccacgc gcacgttgct gacgaaaaca gccatttgca gtatactccc
     9001 gccctaattt ctttaactgg tgcgggcaat ttttgctcgc ttcatcaatg taaggtattc
     9061 cggtgaatat tcaggctctt ctctcagaaa aagtccgtca ggccatgatt gcggcaggcg
     9121 cgcctgcgga ttgcgaaccg cagattcgtc agtcagcaaa agttcagttc ggcgactatc
     9181 aggctaacgg catgatggca gttgctaaaa aactgggtat ggcaccgcga caattagcag
     9241 agcaggtgct gactcatctg gatcttaacg gtatcgccag caaagttgag atcgccggtc
     9301 caggctttat caacattttc cttgatccgg cattcctggc tgaacatgtt cagcaggcgc
     9361 tggcgtccga tcgtctcggt gttgctacgc cagaaaaaca gaccattgtg gttgactact
     9421 ctgcgccaaa cgtggcgaaa gagatgcacg tcggtcacct gcgctctacc attattggtg
     9481 acacagcagt gcgtactctg gagttcctcg gtcacaaagt gattcgcgca aaccacgtcg
     9541 gcgactgggg cactcagttc ggtatgctga ttgcatggct ggaaaagcag cagcaggaaa
     9601 acgccggtga aatggagctg gctgaccttg aaggtttcta ccgcgatgcg aaaaagcatt
     9661 acgacgaaga tgaagagttt gccgagcgtg cacgtaacta cgtggtaaaa ctgcaaagcg
     9721 gtgacgaata tttccgcgag atgtggcgca aactggtcga catcaccatg acgcagaacc
     9781 agatcactta cgatcgtctc aacgtgacgc tgacccgtga tgacgtgatg ggcgaaagcc
     9841 tctacaaccc aatgctgcca ggaattgtgg cggatctcaa agccaaaggt ctggcagtag
     9901 aaagcgaagg ggcgaccgtc gtattccttg atgtgtttaa aaacaaggaa ggcgaaccga
     9961 tgggcgtgat cattcagaag aaagatggcg gctatctcta caccaccact gatatcgcct
    10021 gtgcgaaata tcgttatgaa acactgcatg ccgatcgcgt gctgtattac atcgactccc
    10081 gtcagcatca acacctgatg caggcatggg cgatcgtccg taaagcaggc tatgtaccgg
    10141 aatccgtacc gctggaacac cacatgttcg gcatgatgct gggtaaagac ggcaaaccgt
    10201 tcaaaacccg cgcgggtggt accgtgaaac tggcagacct gctggatgaa gccctggagc
    10261 gtgcacgtcg tctggtggca gaaaagaacc cggatatgcc agctgacgag ctggaaaaac
    10321 tggctaacgc ggttggtatt ggtgcggtga aatatgcgga tctctccaaa aaccgcacca
    10381 cggactacat cttcgactgg gacaacatgc tggcgtttga gggtaatacc gcgccataca
    10441 tgcagtatgc ttacacgcgt gtattgtccg tgttccgtaa agcagaaatt aacgaagagc
    10501 aactggctgc agctccggta atcatccgtg aagatcgtga agcgcaactg gcagctcgcc
    10561 tgctgcagtt tgaagaaacc ctcaccgtgg ttgcccgtga aggcacgccg catgtaatgt
    10621 gtgcttacct gtacgatctg gccggtctgt tctctggctt ctacgagcac tgcccgatcc
    10681 tcagcgcaga aaacgaagaa gtgcgtaaca gccgtctgaa actggcacaa ctgacagcga
    10741 agacgctgaa gctgggtctg gatacgctgg gtattgagac tgtagagcgt atgtaatcga
    10801 tttttcgtga gagtgaagcc tgatcagggt tagccgatca ggctttttta ttgccatcta
    10861 aatgtattct gaaaacggac atgccattgt tttctcactg ttgagtaaga ggccagaagc
    10921 gtaatatcca ggcccaggaa aacgataacg gttcaattta aggaataccg aagtgtttaa
    10981 atttcttgta ttaacattag gcattatctc ttgccaggct tacgcagaag atacggttat
    11041 agtaaacgac catgacactt cagccatcaa agattgttgg caaaaaaatt cagatgatga
    11101 tactgacatt aacgtgatca aatcatgcct gcgacaagaa tacaatctcg tcgatgcgca
    11161 attaaataaa gcctatggtg aagcttatcg ttatatagaa caagtgccac gcacaggtgc
    11221 aaaaaaactt gatactgaac aacttaactt gcttaaaaaa tcacagcgag cctggctgga
    11281 ttttagggac aaagaatgtg aattaatcct ttcaaatgag gatgttcagg atttaagtga
    11341 cccctattct gaatcagaat ggctatcatg tatgatcata cagaccaata cgcgaactcg
    11401 ccagttgcag ctataccgta actctgaaga tttttatcca agccctttga caagaggata
    11461 attcacatct ttttggcatg ttttgttgca agctattcct gataaatgat tgcaacaaaa
    11521 catccagcct tttgactgag ttattaaaca tactcgcgag cgcgtaattt tttgtctttc
    11581 agcgataatt cacaatcact tcattccgtt gtacctttag cggtggaatt aaccgc
//
LOCUS       DAJTRC010000111        11598 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.111, whole genome shotgun sequence.
ACCESSION   DAJTRC010000111 DAJTRC010000000
VERSION     DAJTRC010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11598
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.111"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..2342)
                     /locus_tag="OO357_003342"
     CDS             complement(<1..2342)
                     /locus_tag="OO357_003342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014640177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HCR5888180.1"
                     /translation="MDTQRFQSQFHWHLSFKFSGAIAACLSLSLVGTGLAKADDSLPS
                     SNYAPPAGGTFFLLADSSFSSSEEAKVRLEAPGRDYRRYQMEEYGGVDVRLYRIPDPM
                     AFLRQQKNLHRIVVQPQYLGDGLNNTLTWLWDNWYGKSRRVMQRTFSSQSRQNVTQAL
                     PELHLGNAIIKPSRYVQNNQFSPLKKYPLVEQFRYPLWQAKPVEPQQGVKLEGASSNF
                     ISPQPGNIYIPLGQQEPGLYLVEAMVGGYRATTVVFVSDTVALSKVSGNELLVWTAGK
                     KQGEAKPGSEILWTEGLGVMTRGVTDDSGTLQLQHISPERSYILGKDAEGGVFVSENF
                     FYESEIYNTRLYIFTDRPLYRAGDRVDVKVMGREFRDPLHSSPIVSAPAKLSVLDANG
                     SLLQTVDVTLDARNGGQGSFRLPENAVAGGYELRLAYRNQVYSSSFRVANYIKPHFEI
                     GLALDKKEFKTGEAVSGKLQLLYPDGEPVKNARVQLSLRAQQLSMVGNDLRYAGRFPV
                     SLEGSETVSDASGHVALNLPAADKPSRYLLTVSASDGAAYRVTTTKEILIERGLAHYS
                     LSTAAQYSNSGESVVFRYAALESSKQVPVTYEWLRLEDRTSHSGELPSGGKSFTVNFA
                     KPGNYNLTLRDKDGLILAGLSHAVSGKGSTAHTGTVDIVADKTLYQPGETAKMLITFP
                     EPIDEALLTLERDRVEQQSLLSHPANWLTLQRLNDTLYEARVPVSNSFAPNITFSVLY
                     TRNGQYSFQNAGIKVAVPQLDIRVKTDKTHYQPGELVNVE"
     gene            complement(2276..2899)
                     /locus_tag="OO357_003343"
     CDS             complement(2276..2899)
                     /locus_tag="OO357_003343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1175 domain-containing protein"
                     /protein_id="HCR5888181.1"
                     /translation="MRHGLLALICWLCCVVAHSEMLNVEQSRLFRAWFVRIAQEQLRQ
                     GPSPRWYQQDCAGLVRFAANETLKVHDSKWLKSNGLSSQYLPPEMTLTPEQRQLAQNW
                     NQGSGKNGPYVTAINLIQYNSQFIGQDINQALPGDMIFFDQGDAQHLMVWMGRYVIYH
                     TGSATKTDNGMRAVSLQQLMTWKDTRWIPNDSNPNFIGIYRLNFLAR"
     gene            complement(2896..4584)
                     /locus_tag="OO357_003344"
                     /pseudo
     CDS             complement(2896..4584)
                     /locus_tag="OO357_003344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416733.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2138 domain-containing protein"
     gene            complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="OO357_003345"
     CDS             complement(4733..7360)
                     /gene="gyrA"
                     /locus_tag="OO357_003345"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit A"
                     /protein_id="HCR5888182.1"
                     /translation="MSDLAREITPVNIEEELKSSYLDYAMSVIVGRALPDVRDGLKPV
                     HRRVLYAMNVLGNDWNKAYKKSARVVGDVIGKYHPHGDLAVYNTIVRMAQPFSLRYML
                     VDGQGNFGSIDGDSAAAMRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMP
                     TKIPNLLVNGSSGIAVGMATNIPPHNLTEVINGCLAYIDDEDISIEGLMEYIPGPDFP
                     TAAIINGRRGIEEAYRTGRGKVYIRARAEVEVDAKTGRETIIVHEIPYQVNKARLIEK
                     IAELVKEKRVEGISALRDESDKDGMRIVIEVKRDAVGEVVLNNLYSQTQLQVSFGINM
                     VALHHGQPKIMNLKDIIAAFVRHRREVVTRRTIFELRKARDRAHILEALAVALANIDP
                     IIELIRHAPTPAEAKTALVANPWQLGNVAAMLERAGDDAARPEWLEPEFGVRDGLYYL
                     TEQQAQAILDLRLQKLTGLEHEKLLDEYKELLDQIAELLRILGSADRLMEVIREELEL
                     VREQFGDKRRTEITANSADINLEDLITQEDVVVTLSHQGYVKYQPLSEYEAQRRGGKG
                     KSAARIKEEDFIDRLLVANTHDHILCFSSRGRVYSMKVYQLPEATRGARGRPIVNLLP
                     LEQDERITAILPVTEFEEGVKVFMATANGTVKKTVLTEFNRLRTAGKVAIKLVDGDEL
                     IGVDLTSGEDEVMLFSAEGKVVRFKESSVRAMGCNTTGVRGIRLGEGDKVVSLIVPRG
                     DGAILTATQNGYGKRTAVAEYPTKSRATKGVISIKVTERNGLVVGAVQVDDCDQIMMI
                     TDAGTLVRTRVSEISIVGRNTQGVILIRTAEDENVVGLQRVAEPVDEEDLDTIDGSAA
                     EGDDEIAPEVDVDDEPEEE"
     gene            7507..8229
                     /locus_tag="OO357_003346"
     CDS             7507..8229
                     /locus_tag="OO357_003346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 3-demethylubiquinone
                     3-O-methyltransferase/2-octaprenyl-6-hydroxy phenol
                     methylase"
                     /protein_id="HCR5888183.1"
                     /translation="MNAEKSPENHNVDHEEIAKFEAVASRWWDLEGEFKPLHRINPLR
                     LGYIAERAGGLFGKKVLDVGCGGGILAESMAREGATVTGLDMGFEPLQVAKLHALESG
                     IQVDYVQETVEEHAAKHAGQYDVVTCMEMLEHVPDPQSVVRACAQLVKPGGDVFFSTL
                     NRNGKSWLMAVVGAEYILRMVPKGTHDVKKFIKPAELLGWVDQTSLKERHITGLHYNP
                     ITNSFKLGPGVDVNYMLHTQNK"
     gene            complement(8357..11598)
                     /gene="yfaL"
                     /locus_tag="OO357_003347"
                     /pseudo
     CDS             complement(8357..11598)
                     /gene="yfaL"
                     /locus_tag="OO357_003347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416736.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="AIDA-I family autotransporter adhesin YfaL/EhaC"
BASE COUNT         2666 a         3230 c         2877 g         2825 t
ORIGIN      
        1 cattcgacat tgaccagttc accaggctgg taatgggttt tgtccgtttt cacccggata
       61 tcaagctgag gaacggcaac tttgatcccg gcgttctgaa aactgtactg accattacgg
      121 gtatacagca ccgaaaaagt gatgttaggc gcaaaggaat tgctcactgg tacccgggct
      181 tcatacaggg tatcgtttaa acgttgtagc gttagccagt ttgccggatg cgaaagcagc
      241 gactgctgtt ccacgcgatc gcgttccagc gtcaataacg cttcatcaat tggctccggg
      301 aaagtaatca gcatcttcgc ggtttcgcct ggctggtaca gcgttttatc cgccacgata
      361 tctaccgtac cagtatgcgc cgtgctgccc ttaccgctga cggcatgact taacccagcg
      421 agaattaagc cgtctttatc gcgtaatgtc agattgtagt tgccaggttt agcgaaattg
      481 acggtaaagg atttgccgcc tgacggtagc tctccgctat ggctcgtgcg gtcttcgaga
      541 cgcaaccatt catacgtaac aggaacctgt tttgaagatt ccagcgcggc ataacggaac
      601 acaaccgact cgccgctatt actgtattgt gcggcggtac ttaaagagta atgcgccagg
      661 ccgcgttcaa tgaggatctc tttggtggtg gtgacgcgat acgccgcgcc gtcactggcg
      721 gagacggtta acaaatagcg gctcggttta tcggcggcgg ggagatttaa cgccacatga
      781 ccgctggcgt cggacaccgt ttcgctgcct tccagcgaca cggggaaacg tccggcataa
      841 cgcaaatcgt taccgaccat tgataattgc tgagcgcgca aacttaactg cacgcgggca
      901 ttttttaccg gctcgccatc cgggtagagc agttgcagtt tgccgctgac cgcttcgcca
      961 gttttgaact cttttttgtc gagagctaaa ccaatctcga aatgtggctt gatgtagttt
     1021 gccacgcgaa aactgctgct atagacctga ttgcggtaag caagacgtaa ctcataacct
     1081 ccggcaacgg cattttctgg caggcggaaa cttccctgcc cgccattgcg cgcatccagc
     1141 gtgacatcga cggtttgcaa cagactgccg ttagcgtcca gcaccgaaag cttcgccggg
     1201 gcgctgacga tgggggatga atgcaacgga tcgcggaact cgcggcccat cactttaaca
     1261 tcgacacgat cgcctgcgcg atatagcggg cgatcggtaa aaatatacaa gcgggtgttg
     1321 tagatttcgc tttcgtagaa gaagttctcg gagacaaaaa cgccgccttc agcatcctta
     1381 cccagaatgt atgaacgttc tggcgatata tgttgtaact gcaaagtacc gctgtcatcg
     1441 gtcaccccgc gggtcatcac gccaagacct tcagtccaca agatctctga gccgggcttc
     1501 gcttcaccct gttttttacc cgcggtccat accagaagct cgttacctga cactttgcta
     1561 agcgccacgg tatcggaaac aaacaccacc gtcgtcgccc gatacccacc taccatcgcc
     1621 tcgacgaggt acagccccgg ctcttgttgg ccgagaggaa tataaatatt acccggctgc
     1681 ggcgagatga aattgctgga tgcgccttcc agttttaccc cttgctgcgg ctcgaccggt
     1741 ttagcctgcc atagtggata acggaactgt tccaccaagg gatatttttt cagcggggaa
     1801 aactggttgt tctgtacata acgggaaggt ttaataatgg cattgccgag atgtaattcg
     1861 ggtaatgcct gagtcacatt ctgccgtgac tgagaagaga aagtacgctg catcacgcgg
     1921 cgagatttgc cgtaccagtt atcccacagc caggtcagcg tattgttcag cccgtcgccc
     1981 agatattgcg gttgcaccac aatgcgatgc aggtttttct gctggcgcaa aaatgccatc
     2041 gggtcaggaa tacgatatag gcgaacgtcc acgccgccgt actcttccat ctgatagcgc
     2101 cgataatcac gccccggcgc ttccagtcgc actttcgcct cttcactgct gctaaaactg
     2161 ctgtcagcaa gcaaaaagaa tgttcccccg gcgggcggcg catagttact ggaaggaagc
     2221 gaatcatcag ctttagcaag ccctgttccc acaagagaca gggacaagca cgctgctatc
     2281 gcgccagaaa atttaaacga taaatgccaa tgaaattggg attggaatcg ttgggtatcc
     2341 atcgggtgtc cttccatgtc ataagttgtt gcagactgac tgcgcgcatt ccgttgtcag
     2401 ttttcgtggc gcttccggtg tggtagatga cgtaacgccc catccagacc attaagtgct
     2461 gggcatcgcc ctgatcgaaa aaaatcatat cgccaggcag cgcctggttt atgtcttggc
     2521 caataaactg gctgttgtac tgaatcaaat taatcgcggt cacgtagggg ccgtttttcc
     2581 cgctcccctg attccagttt tgcgccagtt gacgctgttc aggtgttagc gtcatctctg
     2641 gcggcaaata ctggctagat aaaccgttac tttttagcca tttactgtcg tgaactttca
     2701 gcgtctcgtt cgccgcaaat cgcaccaggc ccgcacaatc ctgctgatac cagcgtggac
     2761 ttggcccctg gcggagctgt tcttgtgcaa tgcgtacaaa ccaggcgcga aacagcctgg
     2821 attgttcgac attcagcatt tcgctatggg caacaacaca acacagccag caaatcagcg
     2881 ccagcagccc gtgtctcata gcggctgcca ggttatgggt agccactgcc aggcgacacc
     2941 gggttccatc tgggccagct tcatgacata acgcggttgt tgagataaag cgtccagctt
     3001 cggcattaat aaagtttgtg cggcgttata aaaaaccggt tcgagattct tcggcagact
     3061 ggtcaacgtt tcgttacgca gcagtttcgc cacgccttgt ggattgatat agagcggaac
     3121 gatgccatca gtgggtatta catccaccat tgccgggcgg gttttattca gtgtttgcag
     3181 ggcgttatta accagcgtgt catcgaggga gaaaagcagc gttttgtttt gcatcgccag
     3241 cgacacacgg aaaaaataat ccgacattaa ttgatcgggt tgcgccgcct gcgctttcgg
     3301 atactgaccg tatcgggaac tcacttcgcg acaccagatt tgcgcttcgc cctgctgagt
     3361 ctggcttacc ggcaaaacac cttctggcgc tttgctttcg tgcgcaccaa tattttgcgt
     3421 aaacagtttc cctggcaatt gcgcctgttc ggcagtgcca tcaaactgac cgacaaacag
     3481 cggggtttgc aattttgagt cttcatacca gcacagcccc gcggcaccgt ctaacgcccc
     3541 attcagcttg tcgttttcct ggctgatgtg cgaaagcatc tcttcggcaa taccgtgtga
     3601 atacggcacc gccacacaga agctggctcc ggcaggcata ctgttccaga ccggcgtaaa
     3661 atcgaaactg gcatctacgc tggcggattc atcatttaac gccacaaaac tgtgccagcc
     3721 gtcgttaccc atttcgaagc gtacgccagc aaaagaaggc attaaccgct ggtagccaaa
     3781 acccagcaac ctggcgctga ccacgatgcg caggcgtact ggcgtttttt cggctgtacg
     3841 ctcttccagg ccaaagctgg cttgccagcg ttttttaccg ctcaacaaat cacttgcgat
     3901 cgccgtggct tcggtatcct gctgatcatc tttaaacaac atatccgtac tggaaaacac
     3961 cagcatcttg tcctgatatg tcgcgaacat cagggcgtta ttgccgttat agcgcaactg
     4021 ataaacagga atagtttcac tatttatctt gatgctactg atttccgttt tgcttaacta
     4081 gctgtcgctg gtagcggcaa acaacaatgg ctccagcagt ttgcttaaac cactgcgctg
     4141 gatcagcacc atgtaatgtg aaagatggcc ttgtttatcg tgccatagcg ccgcctgcgc
     4201 gggctgatct aagagtgacg aaaagagctt atctttcagc gtgagatcgt gttcatagac
     4261 aatgcggcga atgctgcctt caatgcccag acgatcggca tgattctgat aatagaaaac
     4321 gaaatcttcg ctcagaacat cgtggagaaa cggaatggtg aggagatctt tgggaagctg
     4381 gctcagagag tcgctgtcta gaaagaggtc cggctcattg agatcgattt gcagattgtt
     4441 gtgcaccacc agcggcgaca acgttttttc tggcccactg ccagcatatt gcaacgccca
     4501 gacgccagcg gaaagcagtg ctattgcgcc aaaacctaca agaccataga accgccagcc
     4561 tttcgccttt ttttcaccac tcattgccac attccttgtg tatagccagc cattttttac
     4621 gggcacagcc aaactttacc gcgccctaat acgacaaaag cccagacttt gcagcctgga
     4681 cttttcaatt caaacaaggg agatagctcc cttttggcat gaagaagtaa aattattctt
     4741 cttctggctc gtcgtcaacg tccacttccg gagcaatttc atcatcccct tccgcggcac
     4801 tgccgtcgat ggtatccaga tcttcctcgt caaccggttc agcaacacgc tgcagaccca
     4861 ctacgttttc atcttccgca gtacggatga ggatcacgcc ctgggtgtta cggcccacga
     4921 tgctgatttc tgaaacgcga gtacgtacca gcgtaccggc atcggtgatc atcatgatct
     4981 ggtcgcagtc atctacctgc accgcgccaa ccactaaacc gttacgttcg gtaaccttga
     5041 tggagataac ccctttcgtc gcacgcgact tggttgggta ttccgccact gcagtacgtt
     5101 taccgtaacc gttttgcgtt gcggtgagga ttgcgccatc gccacgaggc acgatcagag
     5161 agacgacttt atcgccttca cctaagcgaa taccgcgcac accagtggta ttgcagccca
     5221 tcgcacggac agaagactct ttaaagcgca ccactttacc ttcggcggag aacagcatta
     5281 cttcatcttc gccactggtc agatcaacgc cgatcagctc atcgccgtca accagtttga
     5341 tcgccacttt accggcggta cgcagacggt tgaattcggt gaggacggtt ttcttcacag
     5401 taccgttagc ggtcgccatg aagactttca cgccttcttc aaactcggtc accggcagga
     5461 tcgcggtgat acgttcgtcc tgttccagcg gtagcaggtt gacgatcgga cgaccgcgcg
     5521 cgccacgagt ggcttccggc aactggtaaa ctttcatcga atagacgcga ccacggctgg
     5581 agaagcacag aatatggtcg tgcgtgttcg ccaccagcag tcggtcgata aagtcttctt
     5641 ctttaatacg tgcggcagat ttacctttcc cgccacgacg ctgcgcttcg tattcagaaa
     5701 gcggctgata tttaacgtag ccctggtgag agagcgtcac gaccacatct tcctgggtga
     5761 tcagatcttc caggttgatg tctgcgctgt tggcggtgat ttcagtacga cgtttgtcac
     5821 cgaactgttc acgaaccagc tccagctctt cgcggatcac ttccatcaga cgatcggcgc
     5881 taccaagaat acgcaacagt tccgcgatct gatccagcag ctctttgtat tcgtcgagca
     5941 gtttttcgtg ctcaaggccg gtcagtttct gcaaacgcag atccagaatc gcctgagctt
     6001 gctgttcggt caggtagtac agaccatcac gcacgccgaa ctctggctcc agccattccg
     6061 gacgcgcagc atcgtcgcca gcacgttcca acatggcggc aacgttgccc agctgccacg
     6121 gattagcaac cagcgcagtt ttcgcttctg caggcgtcgg cgcatgacgg atcagttcga
     6181 tgatcgggtc aatgttcgcc agcgccacgg ctaatgcttc aaggatatga gcacgatcgc
     6241 gagctttacg cagttcgaaa atagtacgac gggtcaccac ttcacggcgg tgacgaacaa
     6301 acgccgcgat gatgtctttc aggttcatga tcttcggctg accatggtgc aatgccacca
     6361 tgttgatacc gaaagaaacc tgcaactggg tctgggagta gaggttgttg agcacaactt
     6421 caccgaccgc atcgcgtttc acttcaatca cgatgcgcat accgtctttg tcagactcgt
     6481 cacgcagcgc gctgatgcct tccacgcgtt tttcttttac cagttccgca atcttctcga
     6541 tcaggcgcgc tttgtttacc tgatacggaa tttcgtggac gataatggtt tcacgtccgg
     6601 ttttggcgtc aacttccact tcagcacggg cgcggatata taccttgccg cgaccggtac
     6661 ggtaagcttc ttcaataccg cgacgaccgt taatgattgc cgccgtcggg aagtccggcc
     6721 ccgggatgta ttccatcagc ccttcaatgc tgatgtcttc atcatcgata tacgccagac
     6781 aaccgttgat gacttccgtc aggttgtgcg gcgggatgtt ggttgccata cctacggcga
     6841 taccggaaga accgttcacc agcaggttag gaattttggt tggcatgacg tcgggaattt
     6901 tttccgtgcc gtcatagtta tcaacgaaat cgaccgtctc tttttcgaga tcggccatca
     6961 gttcatgggc aattttcgcc agacggattt ccgtataacg cattgccgcc gcagagtcgc
     7021 cgtcgatgga accgaagtta ccctgaccgt ctaccagcat gtaacgcagc gagaatggct
     7081 gcgccatacg gacgatcgtg ttataaaccg ccaagtcacc atggggatgg tatttaccga
     7141 ttacgtcacc aacgacacgg gcagattttt tataggcttt gttccagtca ttgcctagta
     7201 cgttcatggc gtaaagtacg cgacggtgta ccggcttcag gccatctcgg acatctggca
     7261 gcgcacggcc aacaatgacc gacatcgcat aatccagata ggagctcttt agctcttcct
     7321 caatgttgac cggtgtaatt tctctcgcaa ggtcgctcat ctaaccgcta tccctctact
     7381 gtatcccgga ttcaaaggtc gcaaattata acacagccgc gcagtttgag gtaaacctat
     7441 acgctttatt cacatccaat gcctgatata ctcgtttgtc ttgccaatta cggagtagaa
     7501 gtgccaatga atgccgaaaa atcgccggaa aaccataacg tagaccacga agagatcgct
     7561 aaatttgaag ccgtcgcctc ccgctggtgg gatcttgaag gtgagttcaa accgttgcac
     7621 cgcattaacc cgctgcgtct gggctatatt gccgagcgtg ctggcggttt atttggcaaa
     7681 aaggtgctcg atgtcggttg tggcggcggc attctggccg agagtatggc gcgcgaaggc
     7741 gcgacggtga ccggtctgga tatgggcttt gagccattgc aggtggcaaa gctgcacgcg
     7801 ctggaaagcg gcattcaggt ggattacgtg caggaaaccg tggaagagca cgcggcaaaa
     7861 catgccgggc agtatgatgt ggtgacctgc atggagatgc tggagcacgt acccgatccg
     7921 cagtcagtgg tcagagcctg tgcgcaactg gtgaaaccag gcggcgatgt ctttttctcg
     7981 acgcttaacc gcaacggcaa gtcatggctg atggcggtgg ttggcgcgga atatattttg
     8041 cgcatggtgc ccaaaggtac gcatgatgtg aagaagttta ttaaaccggc agagctgctt
     8101 ggctgggtgg atcagaccag tttaaaagag cgacatataa ccgggctaca ttacaacccg
     8161 atcactaatt cctttaagct cggccccggc gtggatgtga actatatgct gcacacgcag
     8221 aataagtgag gttgatgttt ggccgcgcca atgcctgatg cgacgcttgc agcgtcttat
     8281 caggcctaca aatgctctcc gtaggccaga taaggcgttt acgccgcatc cggcaattgt
     8341 gatggtcaga cagcgattac catttcaccg tcatcgacaa aaaccctgcc gtctgggcaa
     8401 aatcatcact ccctttctgc cacgccacgc tgccgcgcag ggacactcgc tgactgatat
     8461 tgcccgtgac tcccactttt atttcacccc gtagcttcac cgcatcgtca ctgatagtgc
     8521 tgccatcttc ttcaatttcc gtcgcatggg gatcgtgata gtaattcaga tctaatgttg
     8581 gtgtgacacc aaccgcggta cgccattcgc tgtgtaaacc cagccgcgtc tgaatatcat
     8641 ccccctgcga ttgtgacacg cgcgcatggt tagcggcggt aaaatcatcc tgctgcacgc
     8701 cctgataaat cacctgcgcc tgcggttcaa tcaccacacc acgccccggt aaccactgat
     8761 acccagcttc cagcgaggcg ataatccccg acgaatggta acgatccacg ccatcttcat
     8821 gttcggaaac atcattgtta aaccacgcgt attgcagcca gctatccagc caggccccct
     8881 gcttctgatt accgtgctga taccagcttg aggtcagccc aacggcataa ccgtggttct
     8941 ggttatcggc gcgagttccg gtcatattcg agcggctgtc gccctgatta tcactgtagc
     9001 caccaaccgc cccaagcatc cactcgccat ccgtgcccca gcgcccgcta aacaagccgc
     9061 cgctaagctg caccgtagaa gtgtcttcct gctgagccag ttgccctgcc gccgtgtaat
     9121 gataacgtcc gccgataaca cgtaaattca gcgtctgacc atcgccacca gcgtgatcgc
     9181 gtcgctccat cataaacgcc tgatttgccg cccgcaggtt attgagataa ccgccaactt
     9241 tggcattcag caccggctgg taagcaggcg taggggccgg gtcgggggtt gggtcaggat
     9301 ccggcgtggg atcaggatcg ggagtcgggt ctggctcagg tggcgatggc ggcgttactt
     9361 cttgcgatcg cagataccag tcgttgttat cttccactag cgtgtagtca tacgctccca
     9421 tgttgacgta gccactgcca gccagactga actgtgcatt gttttgaaac tgcgtgggat
     9481 cagctgcgaa atcaaccact ttaatgcctg tcgatgtcgg ctcaccaatc cctgtaatgg
     9541 agttaaccac cacagttgtg ttgccagcag tattaccgtt catcaccaat tgatcgctta
     9601 ccgagtcatc gccgtttaat tcgctgtcga atagtagcgt accgccgccg gtataatcac
     9661 cgtttaccgt cagcgtatcg ccagcgacgc cattttgcag gctaaccgca ccggacgtat
     9721 tggtcagatc accattaacc gtcacgtcag caagataggt caggctgtca gtttcaaggt
     9781 tggtattaag ctcgcctgtc aacgtcacgc catcggtagc atttaccagc gtcccatcgc
     9841 cgttaaatag tgaggcatta agggcgacgg aagtatcctg cccggtcaga cgcaaaaccg
     9901 taccatcgct gatgtcgata gtgccgctgg aagtagcatc aattgactga atatcctgat
     9961 ccgcgccagt aacgaacgtt gccccatcac caacccacag tgacgaagca taaggaaaga
    10021 tatccgcaac atcgcctttc agggtgcctt cttcgacacg taccgccgac tgcgttgtac
    10081 cgctggcggt cagctccagc gtacccgccc ccgttttagt aaatgtgcta ccctcatcct
    10141 gatgctgccc gctgctgtca gccatcagtg cgccccactg cgtgtctacc cccgcatcaa
    10201 ctgccacttc accgtcggcg cgcatttcaa tatcacgccc atgctcagtc gtcgtagagc
    10261 gagtatcgac attggcagta atctccatca cgctttcttg ttacttatcg gtaaagataa
    10321 cctggcggtt atagtgggta tctccaagct gcgagttgtc gctcaccatc agcgtaccgg
    10381 aggcgatttg tgttgtgccc aaataactat tattgtttgc cagacttacc tgcccgtcgc
    10441 tcgcatcgac caccacgtag acgccatctt tcccgtcgcc gccgatatgc aaattgtcgc
    10501 cacctgcaag atctaccaca tcctgactgc cgataaccgt tccgctgctg ccactgactt
    10561 caaggccatc attgtatgat gtgccgctct gccaagtgga gaaatcagag agatcgagca
    10621 caccgccgtt taacacgatc gactgcggat cgtcctggag agcggtaaga tctgccgcgt
    10681 cgccttccag cgaaagcacc gcaccatcat caacgactat atcgccggtt agcgccatcg
    10741 actgcgcccc tgccagcacg tagctgccgt tttgcgcaat ggtgagctga ccagcacctt
    10801 cgatgatgcc agcaaaactg ccctgattaa cagtaacgtt gccaccagca tcgatattta
    10861 aagtgccatt ctgaaagccc gtcaatgcgt gcacaaaagt ttgttgggtc gagccaacgt
    10921 ttagctcagc ctgattctga tactgatcaa tactccctat cgtcagaccg tagcagtctt
    10981 gcggatcgtc ctggcaatgc gtatcgccga cattcatcag ggagttgctg cgacccaggg
    11041 taacttcacc gttttcaatc tgcatctcac cagtaaagtc attgttatct gcattaagta
    11101 ccagatcgcc ggaacctgtt ttggtgatta acccggtacc agcaatagag tcaacagctc
    11161 cgtcattctc tgtattgccg ataaccagcg tttttccgtc ggcaatatca aaggtaactt
    11221 cacttaagcc gagatacata aagccacccg ccgcagtgga gggaccatct ccatagcctg
    11281 ctgcgctatt gttctcatcg actaacacgc cgccgttctg gctgtagctg tcatcaacag
    11341 aaatatcaat aagataggga gccgtcgcgc tattggtata tatcgccccg ccataacctt
    11401 cggctgtgtt atttgtaaag gcggtgttat ttattatcgt ataacctgaa ggatgcttgc
    11461 tgtcgctatt attatcggta acatcgattg ccccgccatc gccatcactg taacttgttg
    11521 atgtatatgc ctggttattg ttaaaaacat catcacttaa atagacgtca ttattaatgg
    11581 tataaattgc gccacctt
//
LOCUS       DAJTRC010000112        11524 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.112, whole genome shotgun sequence.
ACCESSION   DAJTRC010000112 DAJTRC010000000
VERSION     DAJTRC010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.112"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(217..292)
                     /locus_tag="OO357_003348"
     tRNA            complement(217..292)
                     /locus_tag="OO357_003348"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(257..259),aa:Ile,seq:cat)
     gene            851..3103
                     /locus_tag="OO357_003349"
     CDS             851..3103
                     /locus_tag="OO357_003349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888184.1"
                     /translation="MFSIKPGPGNLPIDNPTLLSWNITDGDLNSKFNTLEYLNCITNI
                     INACGVYPQDLKDREIISTFHAEKVINDLLKNDYKISLSPDTTYRELNKAAQSSITAP
                     DRIGEGKTWVYQRDTMVERGDNSGVHQYGPAEHFTHIISDKPSPKDKYVAYAINIPDY
                     ELAADVYNINVTSPSGQQETFKILINPEHLRQTLERKSLTAVQKSQCEIITPKKPGEA
                     ILHAFNATYQQIRENMSEFARSHYGYIQIPPVTTFRADGPETPEEEKGYWFHAYQPED
                     LCTIHNPMGDLQDFIALVKDAKKFGIDIIPDYTFNFMGIGGSGKNDLDYPSADIRAKI
                     SKDIESGIPGYWQGQVLIPFIKDPVTKERKQIHPEDIHLTAKDFEASKDNISKDEWKN
                     LHALKEKSLNGMPKTTPKSDQVIMLQNQYVREMRKYGVRGLRYDAAKHSKHEQIERSI
                     TPPLKNYNERLHNTNLFNPKYHEKTVMNYMEYLVTCQLNEEQMSSLLYERDDLSAIDF
                     SLLMKTIKAFSFGGDLLTLASKPGSTISSIPSERRILININHDFPNNGNLFNDFLFNH
                     QQDEQLAMAYMAALPFSRPLVYWDGQVLKSTTEIKNYDGPTRVGGEAWLNKGCSTYQQ
                     LYNEFHALYIDKAGIWSAFEGVFATKNVLAFSRGDSVNINHSPHDGLVIINKGNEEVE
                     GTWPNKLQPGIYKNMGSNSVNIIINNTRKIIPPCKVFTLRGGSLNINIPRRSALLLGK
                     TGEPPNYLYL"
     gene            3439..4371
                     /gene="csiD"
                     /locus_tag="OO357_003350"
     CDS             3439..4371
                     /gene="csiD"
                     /locus_tag="OO357_003350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311547.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon starvation induced protein CsiD"
                     /protein_id="HCR5888185.1"
                     /translation="MNALTAVQNNAVDSGQDYSGFTLIPSAQSPRLLELTFTEQTTKQ
                     FLEQVAEWPVQALEYKSFLRFRVGKILDDLCANQLQPLLLKTLLNRAEGALLINAVGI
                     DDVAQADEMVKLATAVAHLIGRSNFDAMSGQYYARFVVKNVDNSDSYLRQPHRVMELH
                     NDGTYVEEITDYVLMMKIDDWEHLDHYFRHPLARRPMRFAAPPSKNVSKDVFHPVFDV
                     DQQGRPVMRYIDQFVQPKDFEEGVWLSELSDAIETSKGILSVPVPVGKFLLINNLFWL
                     HGRDRFTPHPDLRRELMRQRGYFAYATHHYQTHQ"
     gene            4391..5659
                     /gene="lhgO"
                     /locus_tag="OO357_003351"
     CDS             4391..5659
                     /gene="lhgO"
                     /locus_tag="OO357_003351"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311548.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HCR5888186.1"
                     /translation="MYDFVIIGGGIIGMSTAMQLIDVYPDARIALLEKESGPACHQTG
                     HNSGVIHAGVYYTPGSLKAQFCLAGNRATKAFCDQNGIRYDNCGKMLVATSQLEMERM
                     RALWERTAANGIEREWINAVELREREPNITGLGGIFVPSSGIVSYREVTAAMAKIFQA
                     RGGEIIYNAEVSALSEHKNGVVIRTRQGGEYEASTLISCSGLMADRLVKMLGLEPGFI
                     ICPFRGEYFRLAPEHNQIVNHLIYPIPDPAMPFLGVHLTRMIDGSVTVGPNAVLAFKR
                     EGYRKRDFSFSDTLEILSSSGIRRVLQNHLRSGLGEMKNSLCKSGYLRLVQKYCPRLS
                     LSDLQPWPAGVRAQAVSPDGKLIDDFLFVTTPRTIHTCNAPSPAATSAIPIGAHIVSK
                     VQTLLASQSNPGRTLRAARSVDALHAAFNQ"
     gene            5682..7130
                     /gene="gabD"
                     /locus_tag="OO357_003352"
                     /pseudo
     CDS             5682..7130
                     /gene="gabD"
                     /locus_tag="OO357_003352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311549.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent succinate-semialdehyde
                     dehydrogenase"
     gene            7138..8424
                     /gene="gabT"
                     /locus_tag="OO357_003353"
                     /pseudo
     CDS             7138..8424
                     /gene="gabT"
                     /locus_tag="OO357_003353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001307963.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="4-aminobutyrate--2-oxoglutarate transaminase"
     gene            8662..10062
                     /gene="gabP"
                     /locus_tag="OO357_003354"
     CDS             8662..10062
                     /gene="gabP"
                     /locus_tag="OO357_003354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GABA permease"
                     /protein_id="HCR5888187.1"
                     /translation="MGQSSQPHELGGGLKSRHVTMLSIAGVIGASLFVGSSVAIAEAD
                     PAVLLAYLFAGLLVVMIMRMLAEMAVATPDTGSFSTYADKAIGRWAGYTIGWLYWWFW
                     VLVIPLEANIAAMILHSWVPGIPIWLFSLVITLALTGSNLLSVKNYGEFEFWLALCKV
                     IAILAFIFLGAVAISGFYPYAEVSGISRLWDSGGFMPNGFGAVLSAMLITMFSFMGAE
                     IVTIAAAESDMPEKHIVRATNSVIWRISIFYLCSIFVVVALIPWNMPGLKAVGSYRSV
                     LELLNIPHAKLIMDCVILLSVTSCLNSALYTASRMLYSLSRRGDAPAVMGKINRSKTP
                     YVAVLLSTGAAFLTVVVNYYAPAKVFKFLIDSSGAIALLVYLVIAVSQLRMRKILRAE
                     GSEIRLRMWLDPWLTWLVIGFITFVLVVMLFRPAQQLEVISTGLLAIGIICTVPIMAR
                     WKKLVLWQKTPVHNTR"
     gene            10083..10745
                     /gene="csiR"
                     /locus_tag="OO357_003355"
     CDS             10083..10745
                     /gene="csiR"
                     /locus_tag="OO357_003355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator CsiR"
                     /protein_id="HCR5888188.1"
                     /translation="MTITSLDGYRWLKNDIIRGNFQPDEKLRMSLLTSRYALGVGPLQ
                     EALSQLVAERLVTVVNQKGYRVASMSEQELLDIFDARANMEAMLVSLAIARGGDEWEA
                     DVLAKAHLLSKLEACDASEKMLDEWDLRHQAFHTAIVAGCGSHYLLQMRERLFDLAAR
                     YRFIWLRRTVLSVEMLEDKHDQHQTLTAAVLPRDTARASELMRQHLLTPIPIIQQAMA
                     GN"
     gene            complement(10746..11195)
                     /gene="kbp"
                     /locus_tag="OO357_003356"
     CDS             complement(10746..11195)
                     /gene="kbp"
                     /locus_tag="OO357_003356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium binding protein Kbp"
                     /protein_id="HCR5888189.1"
                     /translation="MGLFNFVKDAGEKLWDAVTGQHDKDDQAKKVQEHLSKTGIPDAD
                     KVNIQIADGKATVTGDGLSQEAKEKILVAVGNISGIASVDDQVKTATPATASQFYTVK
                     SGDTLSAISKQVYGNANLYNKIFEANKPMLKSPDKIYPGQVLRIPEE"
     gene            complement(11279..11437)
                     /locus_tag="OO357_003357"
     CDS             complement(11279..11437)
                     /locus_tag="OO357_003357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaE/Pmp3 family membrane protein"
                     /protein_id="HCR5888190.1"
                     /translation="MGFWRIVITIILPPIGVLLGKGFGWAFIINILLTLLGYIPGLIH
                     AFWVQTRD"
BASE COUNT         2962 a         2830 c         2874 g         2858 t
ORIGIN      
        1 tttgcccgct tggcatagcg ggcatttttt ccaggtactt ttgaatgagt actgatgaat
       61 aaatacattg cagtggcgtg ccacgtacca aaacaccagc cctcattcga aaccacccac
      121 cgcacttctt ccttgaaatg gcgttagtca tgaaatatag accgccatcg agtacccctt
      181 gtacccttaa ctcttcctga tacatgaatg atgatttggt ggcccttgct ggacttgaac
      241 cagcgaccaa gcgattatga gtcgcctgct ctaaccactg agctaaaggg ccttaagtat
      301 gcaataacaa tacttataaa ccacgcaata aacatgatga tcttatgatg taaataacag
      361 atttttatgc gttcccattc accttgggtc gtgattacac gcgacataaa acccgacaca
      421 gcctccattc gcaaagtcga tactcgcagt caacaagcaa atgttaataa ttagcactat
      481 ctatagttat catcgattca atgatacttt gtaatgattt tgtatctaat aatataactt
      541 tattacatta gctgaagagt tttcgcatca ttatgatatc tgttactttt cactccataa
      601 aaataaactt catatagcaa tatattcttt catagatctt attctgctaa tcattagttt
      661 cgtatgcgcg atttttgaca gttgcctctc cagaccacat cgataattaa aaaacagatt
      721 taaacattat ccttttccat atagatattg gataaaaata ggacatctgt ttgcaattac
      781 tttcacaaca attaaacatt tttatgtttt cgtatacatc atattactct accattagag
      841 gaacgttatt atgttttcta taaaaccagg acctggaaat ttacctatcg acaaccccac
      901 attgttatca tggaacatta ctgatgggga tctaaattcc aaatttaata cattagaata
      961 tctaaactgt ataacaaata ttattaatgc ttgtggagtt tatcctcagg atttaaaaga
     1021 cagagaaatt atatcaactt ttcacgcaga aaaagtcatt aatgatctgt taaaaaacga
     1081 ttataaaatt tccctttctc cagatacaac ttaccgagag ctgaataaag ctgcacagag
     1141 tagcattaca gcgccagaca ggataggaga agggaaaaca tgggtttatc aacgagatac
     1201 aatggttgaa agaggtgata acagtggtgt tcatcagtat ggtccagctg aacatttcac
     1261 tcacattata tctgacaaac cgtccccaaa agataagtat gttgcatatg ctattaacat
     1321 tcctgactat gagctagcag ccgatgtata taatattaac gtgacgtcac cttccggaca
     1381 gcaagaaaca tttaaaatac taatcaatcc agaacatcta cggcaaacac ttgagcgtaa
     1441 atctcttact gctgttcaga aatcacaatg tgaaatcatc acccccaaaa aacctggcga
     1501 agcgattctt catgctttta atgccaccta ccagcaaatc agggaaaata tgtctgagtt
     1561 tgcacgttcc cattatgggt atatacaaat ccctccagtg acaactttcc gcgccgacgg
     1621 accagaaact cccgaagaag aaaaaggtta ctggtttcac gcttatcaac ccgaagatct
     1681 ttgtaccatc cataatccaa tgggggattt gcaggatttt atcgcattgg ttaaagatgc
     1741 taaaaaattt ggtatcgata tcattcctga ttataccttt aactttatgg gaatcggggg
     1801 aagtggtaaa aatgacctgg attatccctc tgctgatata cgagcgaaga tcagtaaaga
     1861 tatagaaagt ggtatccctg gctattggca aggtcaggtt ttgattccat tcattaaaga
     1921 tccagtaaca aaagaacgta aacaaatcca tccagaagat atacatctca ctgcaaaaga
     1981 cttcgaagca agtaaagata acatctctaa ggatgaatgg aaaaacctcc atgcattaaa
     2041 agaaaagagt ttaaatggaa tgcctaaaac aacccccaaa agtgaccagg ttattatgtt
     2101 gcaaaatcaa tacgttcgtg aaatgcgaaa atatggcgta cgaggtttac gttatgatgc
     2161 agcaaaacac tcaaaacatg aacaaataga aagatcaata accccaccgc ttaaaaatta
     2221 taatgagcga ttacacaata ctaacttatt taacccaaaa tatcatgaaa aaaccgttat
     2281 gaattacatg gaatatctgg taacttgtca gttgaatgaa gaacaaatgt catcgctgct
     2341 ttatgaaaga gatgatttaa gcgccattga tttttcattg ctcatgaaga cgataaaagc
     2401 cttttcattc gggggagatc tcctaaccct tgcatcaaaa ccgggttcca caatctcaag
     2461 catcccatca gaaagacgga tattgattaa cattaaccac gattttccta acaatggcaa
     2521 tcttttcaat gactttctat ttaaccatca acaagatgaa caattagcaa tggcatatat
     2581 ggccgctctc ccgttcagca ggcctttagt ttactgggat ggccaggtat taaaatcaac
     2641 gactgaaatt aaaaattatg atgggcccac gcgtgtcggt ggtgaggcgt ggcttaataa
     2701 aggttgctct acctatcagc agctctacaa tgaattccac gcattatata tagataaagc
     2761 aggaatatgg agcgcatttg agggtgtatt tgcaactaaa aacgttctgg cctttagtcg
     2821 tggggattct gtgaacatta atcactctcc tcatgatgga ctagttataa taaataaagg
     2881 aaacgaagaa gttgaaggta cctggcctaa caaattgcaa cctggaatat ataaaaacat
     2941 ggggagtaat agcgttaaca ttattattaa taatacccga aaaattatcc ccccttgtaa
     3001 agtatttacg cttagaggcg gatctctaaa tatcaatatt cctaggcgta gcgctcttct
     3061 tttagggaaa acaggagaac cgccgaacta tctctattta taatttaatg ttatatctgc
     3121 cccgataaaa cggggcagat aatatgttta gtttactaac ggtcattttg tagtgaagcc
     3181 atttactgtt ttttatcgac cagataatct gttctctaat gttaactccc cctaacctgt
     3241 tgctttagtt attcatttcc tgtctcactt tgccttaata ccctacgtta aatgttacta
     3301 atttgttgct tttgatcaca ataagaaagc aatatgtcgc ttttgtgcgc atttttcaga
     3361 aatgtagata tttttagatt atggctacga aatgagcatc gccatgtcac cctacatctc
     3421 ataagaggat cgcttctgat gaatgcactg accgccgtac aaaataacgc tgtcgattca
     3481 ggccaggact atagcggatt cactctcatc ccgtcggcgc aatccccgcg tctgctggaa
     3541 ctcaccttca ccgaacagac gaccaaacag tttctcgaac aggttgccga atggcccgtg
     3601 caggcgctgg agtacaaatc gtttctgcgt tttcgggtag gcaaaattct tgacgatctg
     3661 tgtgcgaatc agctgcaacc attgctgttg aagaccctgc taaaccgtgc tgaaggtgcg
     3721 ctgttgatca atgcggtagg tatcgatgat gtggcgcagg cagatgagat ggtgaagctg
     3781 gcaacggcgg tagcgcatct gattggtcgc tcaaatttcg atgcgatgag cggtcagtat
     3841 tacgcgcgtt tcgtggtgaa aaatgtcgat aactcagaca gctatctgcg tcagccgcac
     3901 cgcgtgatgg aactgcacaa cgacggcacg tatgtcgaag agatcaccga ttacgtgctg
     3961 atgatgaaaa tcgatgactg ggaacatctg gaccactatt tccgccatcc actggcgcgt
     4021 cgcccgatgc gctttgccgc gccgccgagc aaaaacgtca gcaaagatgt tttccatcca
     4081 gtgttcgacg ttgatcaaca gggtcgcccg gtgatgcgct atatcgacca gttcgtccag
     4141 ccaaaagatt ttgaagaagg cgtgtggttg agcgagcttt ccgatgccat tgaaaccagc
     4201 aaaggcattc tttctgtacc cgttcccgtt ggcaaattcc tgttgataaa caacctgttc
     4261 tggctgcacg gtcgcgaccg ctttactccg cacccggatc tgcgccgtga actgatgcgt
     4321 cagcgtggct atttcgctta cgccactcac cactaccaga cgcatcagta agcgcaaagg
     4381 aattgagcgg atgtatgatt ttgtgattat tggcggcggc atcatcggca tgtcgaccgc
     4441 catgcaactg attgatgttt atccggatgc ccgcattgcg ttgctggaaa aagagtccgg
     4501 cccggcctgt caccagacgg gccacaacag cggcgtgatc catgccgggg tctattacac
     4561 gcccggcagc ctgaaggcgc agttttgcct ggcggggaac cgtgccacca aagccttttg
     4621 cgatcaaaac ggcattcgct atgacaactg cggcaagatg ctggtcgcca cgtctcagct
     4681 cgaaatggaa cggatgcgcg ccttatggga acgcacagcg gcgaacggta tcgagcgcga
     4741 gtggataaac gccgtggaac tgcgcgagcg cgaaccgaat atcaccgggc ttggtggcat
     4801 ttttgtgccg tccagcggca ttgtcagcta ccgcgaagtg acggcggcaa tggcaaaaat
     4861 cttccaggcc agaggcggcg agattattta taacgccgaa gtcagcgccc tcagtgagca
     4921 taaaaacggc gtggtgatac gcacccgtca gggcggtgaa tatgaagcat cgacgctgat
     4981 tagctgttcc gggctgatgg ctgaccggct ggtgaaaatg ctcggcctcg aaccgggctt
     5041 tattatctgc ccgttccgtg gcgagtattt ccgccttgcg ccggagcata accagattgt
     5101 taaccacctg atttacccca ttcccgaccc cgcgatgcca tttttgggcg tgcatctcac
     5161 ccgcatgatc gacggcagcg tgaccgtcgg gccaaacgcg gtgctggctt tcaaacgcga
     5221 aggctatcgc aagcgcgact tctcatttag tgacacgctg gagattttga gctcgtcggg
     5281 gattcgccgg gtgctgcaaa accatctacg ctcaggactg ggcgagatga aaaactcgct
     5341 gtgcaaaagc ggctatctgc ggctggtgca aaagtattgt ccccggcttt cgttaagcga
     5401 tctccagccc tggcccgccg gtgtgcgggc gcaggcagta tcgccggacg gcaagctgat
     5461 tgacgatttt ctgtttgtca ccaccccacg cacgatccac acctgcaatg cgccctcccc
     5521 ggcagcgaca tcagcaattc ctattggtgc gcatattgtc agcaaggtac aaacgctgtt
     5581 ggcaagccag agtaaccccg gacgcacgct gcgagcggca cgtagtgtgg atgccttaca
     5641 cgccgcattt aatcaataac ctttgaaaac aggacgtaac aatgaaactt aacgacagta
     5701 acttattccg ccagcaggcg ttgattaacg gggaatggct ggacgccaac aatggtgaag
     5761 tcatcgacgt caccaatccg gcgaacggcg acaagctggg tagcgtgccg aaaatgggcg
     5821 cagatgaaac ccgcgccgct atcgacgccg ccaaccgcgc cctgcccgcc tggcgtgcgc
     5881 tcaccgccaa agaacgcgcc accattctgc gcaactggtt caatttgatg atgcagcatc
     5941 aggacgattt agcgcgcctg atgaccctcg aacagggtaa accgctggct gaagcgaaag
     6001 gcgaaatcag ctacgccgcc tccttcattg agtggtttgc tgaagaaggc aaacgtattt
     6061 atggcgacac cattcctggt catcaggccg ataagcgcct gattgttatc aagcagccga
     6121 ttggcgtcac cgccgccatc acgccgtgga actttccggc ggcgatgatt acccgtaaag
     6181 ccggtccggc gctggcggca ggctgcacca tggtgctgaa gcccgccagt cagacgccgt
     6241 tctctgcgct ggcgctggcg gaactggcga ttcgcgcggg cattccggct ggcgtgttta
     6301 acgtggtcac cggttcggca ggcgtggtcg gtaatgaact gaccagcaac ccgctggtac
     6361 gcaaactgtc gtttaccggt tagaccgaga ttggccgcca gttaatggaa cagtgcgcga
     6421 aagacatcaa aaaagtgtcg ctggagctgg gcggcaacgc gccgtttatc gtctttgacg
     6481 atgccgatct cgataaagcc gtggaaggcg cgctggcctc gaaattccgc aacgccgggc
     6541 aaacctgcgt ctgcgccaac cgtttatacg tgcaggacgg cgtgtatgac cgctttgccg
     6601 aaaaattaca gcaggcgggg agcaaactgc acatcggcga cgggctggag aaaggcgtca
     6661 ccatcgggcc gctgatcgat gaaaaagcgg tagcaaaagt ggaagagcat attgccgatg
     6721 cgctggagaa aggtgcgcgt gtggtttgcg gcggtaaagc gcacgaacgc ggcggcaact
     6781 tcttccagcc aaccattctg gtggacgttc cggccaacgc caaagtgtcg aaagaagaga
     6841 cgttcggccc cctcgcccca ctgttccgtt ttaaagatga agccgatgtg attgcacaag
     6901 ccaatgacac cgagtttggt cttgccgcct atttctacgc ccgtgattta agccgcgtct
     6961 tccgcgtcgg cgaggcgctg gagtacggca tcgtcggcat caataccggc attatttcca
     7021 atgaagtggc cccattcggc ggcatcaaag cctcgggtct gggtcgtgaa ggttcgaagt
     7081 atggcatcga agattactta gaaatcaaat atatgtgcat cggtctttaa ctggagaatg
     7141 cgaaggaaca gcaataaaga gttaatgcag cgccgcagtc aggcgattcc ccgtggcgtt
     7201 gggcaaattc acccgatttt cgctgaccgc gcggaaaact gccgggtgtg ggacgtttaa
     7261 ggccgtgagt atcttgattt cgcgggcggg attgcggtgc tcaataccgg gcacctgcat
     7321 ccgaaggtgg tggccgcggt ggaagcgcag ttgaaaaaac tgtcgcacac ctgcttccag
     7381 gtgctggctt acgagccgta tctggagctg tgcgagatta tgaatcagaa ggtgccgggc
     7441 gatttcgcca agaaaacgct gctggttacg accggttccg aagcggtgga aaacgcggta
     7501 aaaatcgccc gcgccgccac caaacgtagc ggcaccatcg cttttagcgg cgagtatcac
     7561 gggcgcacgc attacacgct ggcgctgacc ggcaaggtga atccgtactc tgcgggcatg
     7621 gggctgatgc cgggtcatgt ttatcgcgcg ctttatcctt gcccgctgca cggcataagc
     7681 gaggatgacg ctatcgccag catccaccgg atcttcaaaa atgatgccgc gccggaagat
     7741 atcgccgcca tcatgattga gccggttcag ggcgaaggcg gtttctacgc cgcgtcgcca
     7801 gcctttatgc agcgtttacg cgctctgtgt gacgagcacg ggatcatgct gattgccgat
     7861 gaagtgcaga gcggcgcggg gcgtaccggc acgctgtttg cgatggagca gatgggcgtg
     7921 gcgccggatc ttaccacctt tgcgaaatcg atcgcaggcg gtttcccgct ggcgggcgtc
     7981 accgggcgcg cggaagtgat ggacgctgtt gcaccgggcg gtctgggcgg cacctatgcc
     8041 ggtaacccga ttgcctgcgt ggctgcgctg gaagtgttga aggtgttcga gcaggaaaat
     8101 ctgctgcaaa aagccaacga tctggggcag aagctaaaag acggattgtt ggcgatagcc
     8161 gaaaaacacc cggagatcgg cgacgtacgc gggctgggat cgatgattgc catcgagctg
     8221 tttgaagatg gcgatcacag caaaccggac gcaaaactca ccgccgagat cgtggcacgc
     8281 gcccgcgata agggcctgat tcttctctcc tgcggcccgt attacaacgt gctgcgcatc
     8341 cttgtaccac tcaccattga agacgctcag atccgtcagg gtctggagat tatcggtcag
     8401 tgttttgatg aggcgaagca gtagcaccgt ccccatgcag gaggcgacgc tgcgcgtctt
     8461 gtccggccta cgagaatcca agtcggataa ggcgtttacg ccgcatccga caatctgtac
     8521 gtgaacagga ataaatctat gtcggccgga taaggcggag ccgctctccg gcaaaaagaa
     8581 tcaataacaa ttaaacgcgt gacccgacgc gggaaatgtc ggggcgctct cccaagtgac
     8641 acactttcga gaggattcag gatggggcaa tcatcgcaac cacacgagtt aggcggcggg
     8701 ctgaaatcac gccacgtcac catgttgtct attgccggtg ttatcggcgc aagtctgttt
     8761 gtcggttcca gcgtcgccat cgccgaagcg gacccggcgg tattactggc ctatctgttc
     8821 gccggattac tggtggttat gattatgcgg atgctggcgg aaatggcagt tgccacgccc
     8881 gataccggtt cgttttccac ctatgccgat aaagccattg gccgctgggc gggctatacc
     8941 atcggctggt tgtactggtg gttttgggta ctggttatcc cactggaagc caacatcgcc
     9001 gccatgatcc tgcactcgtg ggttccaggc attcccatct ggttattttc cctcgtcatt
     9061 accctcgcct taactggcag taatttatta agcgttaaaa actacggcga atttgagttc
     9121 tggctggcgc tgtgcaaagt catcgctatc ctggccttta ttttccttgg tgcagtcgca
     9181 attagcggtt tttaccctta tgccgaagtg agcgggatct caagattgtg ggatagcggc
     9241 ggctttatgc ccaacggttt cggtgcggta ttaagcgcga tgttgatcac catgttctcg
     9301 tttatgggcg cagaaattgt caccattgcc gccgcggaat ccgacatgcc ggaaaaacat
     9361 attgtccgcg ccaccaactc ggttatctgg cgtatttcta tcttctattt gtgctctatt
     9421 tttgtcgtcg tggcattaat accgtggaat atgccggggc tgaaagccgt tggatcttat
     9481 cgctcggttc tggaattgct caatattccc catgcgaaat taatcatgga ctgtgtgata
     9541 ttactttccg taaccagttg cctgaactcg gccctgtata ccgcctcaag gatgctctac
     9601 tccttaagcc gtcgcggtga tgctcccgcg gtaatgggta aaatcaaccg cagtaaaacc
     9661 ccgtacgtgg cggtgttact ctccaccggc gcagcattct taacggtggt ggtgaactat
     9721 tacgcgcctg cgaaggtatt taaatttctg atcgacagct ccggcgctat cgccctgctg
     9781 gtttatttag tcatcgccgt ttcacagttg cggatgcgca aaattctgcg agcagaagga
     9841 agcgaaattc gcttgcgaat gtggcttgat ccctggctca cctggctggt catcggcttt
     9901 attacctttg tgttggtagt gatgctattc cgtccggcgc aacagttaga agtgatctcc
     9961 accggcttat tagcgatagg gattatctgt accgtgccaa ttatggctcg ctggaaaaag
    10021 ctggtattgt ggcaaaaaac acccgttcat aatacgcgct gataatgatc aggagtcaca
    10081 ccatgaccat tacgtctctg gatggctatc gctggctgaa gaacgatatt attcgcggta
    10141 attttcaacc ggatgaaaaa ttacggatga gtttactgac atcgcgttat gcacttggcg
    10201 ttgggccgtt gcaggaagca ctttctcaac tggtggcgga acggctggtc acggtggtga
    10261 atcaaaaagg gtatcgggtg gcgtctatgt cagagcagga gctgctcgat attttcgacg
    10321 cccgcgccaa tatggaagcg atgctggtca gtctggcgat tgcccgcggt ggcgatgaat
    10381 gggaggcaga cgttctcgca aaagcgcatc tgctcagtaa gctggaagcc tgtgacgcca
    10441 gcgagaaaat gcttgatgag tgggatctgc gtcatcaggc gtttcataca gcaattgtgg
    10501 cgggctgtgg ttctcactat ttgctgcaaa tgcgtgaacg gttgtttgat ctggcggcgc
    10561 gttatcgatt tatctggctg cggcgaacgg tgctttcggt ggaaatgctg gaggataaac
    10621 acgatcagca ccagaccctg actgcggcgg tactgccgcg agatacagcg cgcgccagtg
    10681 agttaatgcg ccagcattta ctgacgccaa ttcccattat ccagcaggcg atggctggca
    10741 attaactact cttccggaat acgcaacact tgccccggat aaattttatc cgggcttttt
    10801 agcatcggtt tattcgcttc gaagatttta ttgtacagat tagcgttacc gtagacctgt
    10861 ttggaaatgg cactcagagt gtcgccagac ttaacggtat aaaactggct ggcagtggct
    10921 ggtgtcgccg ttttcacctg atcatcaaca ctggcaatac cggaaatatt ccccaccgca
    10981 acaaggattt tctccttcgc ctcctgactc aggccgtcac cagtgaccgt cgctttgccg
    11041 tcggcaattt gaatgttcac tttatcggca tcaggtatac cggttttgct cagatgctcc
    11101 tgcaccttct tcgcctgatc gtctttatcg tgctgacctg taaccgcatc ccagagtttt
    11161 tctccggcat ctttaacaaa attgaacaga cccatagcta cctcattcgt taacggaaac
    11221 actcagaaag tgtagcagag gaacgcggcg cgttaacgcc gcgggggtga gcacaggttt
    11281 aatcgcgggt ttgcacccag aacgcgtgaa tcaaaccggg gatataccca agcagtgtca
    11341 gcagaatatt aataatgaac gcccaaccga accctttacc gagcagcacg ccgatcggcg
    11401 gcagaatgat ggtgatgacg attctccaga aacccatatg tactccctat aagaaaatta
    11461 ctcattgttt aaaaagagat tttatctctt aagcgtagta attttagcgg agcctgccag
    11521 tttc
//
LOCUS       DAJTRC010000113        11478 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.113, whole genome shotgun sequence.
ACCESSION   DAJTRC010000113 DAJTRC010000000
VERSION     DAJTRC010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11478
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.113"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            373..2013
                     /gene="trg"
                     /locus_tag="OO357_003358"
     CDS             373..2013
                     /gene="trg"
                     /locus_tag="OO357_003358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein Trg"
                     /protein_id="HCR5888191.1"
                     /translation="MNTTPSQRLGFLHHIRLVPLFACILGGILVLFALSSALAGYFLW
                     QADRDQRDVTAEIEIRTGLANSSDFLRSARINMIQAGAASRIAEMEAMKRNIAQAESE
                     IKQSQQGYRAYQNRPVKTPADEAFDTELNQRFQAYITGMQPMLKYAKNGMFEAIINHE
                     SEQIRPLDNAYTDILNKAVKIRSTRANQLAELAHQRTRLGGMFMIGAFVLALVMTLIT
                     FMVLRRIVIRPLQHAAQRIEKIASGDLTMNDEPAGRNEIGRLSRHLQQMQHSLGMTVG
                     TVRQGAEEIYRGTSEISAGNADLSSRTEEQAAAIEQTAASMEQLTATVKQNADNAHHA
                     SKLAQEASIKASDGGQTVSGVVKTMGAISTSSKKISEITAVINSIAFQTNILALNAAV
                     EAARAGEQGRGFAVVASEVRTLASRSAQAAKEIEGLISESVRLIDLGSDEVATAGKTM
                     STIVDAVASVTHIMQEIAAASDEQSRGITQVSQAISEMDKVTQQNASLVEEASAAAVS
                     LEEQAARLTEAVNVFRLNKHSVSAEPRGAGEPVSFATV"
     gene            complement(2051..2974)
                     /locus_tag="OO357_003359"
     CDS             complement(2051..2974)
                     /locus_tag="OO357_003359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5888192.1"
                     /translation="MEKNSLFSQRIRLRHLHTFVAVAQQGTLGRAAETLNLSQPALSN
                     TLNELEQLTGARLFERGRQGAQLTLPGEQFLTHAVRVLDAINTAGQSLHRKEGLNNDV
                     VRVGALPTAALGILPSVIGQFHQQQKETTLQVATMSNPMILAGLKTGEIDIGIGRMSD
                     PELMTGLNYELLFLESLKLVVRPNHPLLQENVTLSRVLEWPVVVSPEGTAPRQHSDAL
                     VQSQGCKIPSGCIETLSTSLSRQLTVEYDYVWFVPSGAVKDDLRHATLVALPVPGHGA
                     GEPIGILTRVDATFSSGCQLMINAIRKSMPF"
     gene            3191..4534
                     /locus_tag="OO357_003360"
     CDS             3191..4534
                     /locus_tag="OO357_003360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HCR5888193.1"
                     /translation="MANSITADEIREQFSQAMSAMYQQEVPQYGTLLELVADVNLAVL
                     ENNPQLHEKMVNADELARLNVERHGAIRVGTAQELATLRRMFAIMGMYPVSYYDLSQA
                     GVPVHSTAFRPIDDASLARNPFRVFTSLLRLELIENEILRQKAAEILRQRDIFTPRCR
                     QLLEEYEQQGGFNETQAQEFVQEALETFRWHQLATVDEETYRALHNEHRLIADVVCFP
                     GCHINHLTPRTLDIDRVQSMMPECGIEPKILIEGPPRREVPILLRQTSFKALEETVLF
                     AGQKQGTHTARFGEIEQRGVALTPKGRQLYDDLLRNAGTGQDNLTHQMHLQETFRTFP
                     DSEFLMRQQGLAWFRYRLTPSGEAHRQAIHPGDDPQPLIERGWVVAQPITYEDFLPVS
                     AAGIFQSNLGNETQTRSHGNASREAFEQALGCPVLDEFQLYQEAEERSKRRCGLL"
     gene            4759..6414
                     /gene="opgD"
                     /locus_tag="OO357_003361"
                     /gene_synonym="mdoD"
     CDS             4759..6414
                     /gene="opgD"
                     /locus_tag="OO357_003361"
                     /gene_synonym="mdoD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein OpgD"
                     /protein_id="HCR5888194.1"
                     /translation="MDRRRFIKGSMAMAAVCGTSGIASLFSQAAFAADSDIADGQTQR
                     FDFSILQSMAHDLAQTAWRGAPRPLPDTLATMTPQAYNSIQYDAEKSLWHNVENRQLD
                     AQFFHMGMGFRRRVRMFSVDPATHLAREIHFRPELFKYNDAGVDTKQLEGQSDLGFAG
                     FRVFKAPELARRDVVSFLGASYFRAVDDTYQYGLSARGLAIDTYTDSKEEFPDFTAFW
                     FDTVKPGATTFTVYALLDSASITGAYKFTIHCEKSQVIMDVENHLYARKDIKQLGISP
                     MTSMFSCGTNERRMCDTIHPQIHDSDRLSMWRGNGEWICRPLNNPQKLQFNAYTDNNP
                     KGFGLLQLDRDFSHYQDIMGWYNKRPSLWVEPRNKWGKGTIGLMEIPTTGETLDNIVC
                     FWQPEKAVKAGDEFAFQYRLYWSAQPPVHCPLARVMATRTGMGGFPEGWAPGEHYPEK
                     WARRFAVDFVGGDLKAAAPKGIEPVITLSSGEAKQIEILYIEPIDGYRIQFDWYPTSD
                     STDPVDMRMYLRCQGDAISETWLYQYFPPAPDKRQYVDDRVMS"
     gene            6554..6778
                     /locus_tag="OO357_003362"
     CDS             6554..6778
                     /locus_tag="OO357_003362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415943.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcH family protein"
                     /protein_id="HCR5888195.1"
                     /translation="MFPEYRDLISRLKNENPRFMSLFDKHNKLDHEIARKEGSDGRGY
                     NAEVVRMKKQKLQLKDEMLKILQQESVKEV"
     gene            6841..7377
                     /gene="rimL"
                     /locus_tag="OO357_003363"
     CDS             6841..7377
                     /gene="rimL"
                     /locus_tag="OO357_003363"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707645.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12-serine
                     acetyltransferase"
                     /protein_id="HCR5888196.1"
                     /translation="MTETIKVSESLELHAVAESHVTPLYQLICKNKTWLQQSLNWPQF
                     VQSEEDTRKTVQGNVMLHQRGYAKMFMIFKEDELIGVISFNRIEPLNKTAEIGYWLDE
                     SHQGQGIISQALQTLIHHYAQSGELRRFVIKCRVDNPQSNQVALRNGFILEGCLKQAE
                     FLNDAYDDVNLYARIIDS"
     gene            complement(7372..8352)
                     /locus_tag="OO357_003364"
     CDS             complement(7372..8352)
                     /locus_tag="OO357_003364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888197.1"
                     /translation="MRKYRLSEEQRAFSYQEDGTKKSVLLRQIIAISDFNDVIAGTAG
                     GWIDRETVLAQEGNCWIYDQNAIAFGGTVISGNTRITGTSVLWGEVYATDNVWIDNSE
                     ISQGAYISDSVTIHDSLVYVQCRIFGHALIDQHSMIVAAQGLTPDHQLLLQIYDRARV
                     SASRIVHQAQIYGDAVVRYAFIEHRAEVFDFASVEGNEENNVWLCDCAKVYGHAQVKA
                     GIEEDAIPTIHYSSQVAEYAIVEGNCVLKHHVLIGGNAVVRGGPILLDEHVVIQGESR
                     ITGAVIIENHVELTDHAVVEAFDGDTVHVRGPKVINGEERITRTPLAGLL"
     gene            8476..9468
                     /gene="tehA"
                     /locus_tag="OO357_003365"
     CDS             8476..9468
                     /gene="tehA"
                     /locus_tag="OO357_003365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707643.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dicarboxylate transporter/tellurite-resistance
                     protein TehA"
                     /protein_id="HCR5888198.1"
                     /translation="MQSDKVLNLPAGYFGIVLGTIGMGFAWRYASQVWQVSHWLGDGL
                     VILAMIIWGLLTSAFITRLIRFPHSVLAEVRHPVLSSFVSLFPATTMLVAIGFVPWFR
                     PLAVCMFSFGVVVQLAYAAWQTAGLWRGSHPEEATTPGLYLPTVANNFISAMACGALG
                     YTDAGLVFLGAGVFSWLSLEPVILQRLRSSGELPTALRTSLGIQLAPALVACSAWLSV
                     NGGEGDTLAKMLFGYGLLQLLFMLRLMPWYLSQPFNASFWSFSFGVSALATTGLHLGS
                     GSDNGFFHTLAVPLFIFTNFIIAILLIRTFALLMQGKLLVRTERAVLMKAEDKE"
     gene            9465..10058
                     /gene="tehB"
                     /locus_tag="OO357_003366"
     CDS             9465..10058
                     /gene="tehB"
                     /locus_tag="OO357_003366"
                     /EC_number="2.1.1.265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tellurite resistance methyltransferase TehB"
                     /protein_id="HCR5888199.1"
                     /translation="MIIRDENYFTDKYELTRTHSEVLEAVKVVKPGKTLDLGCGNGRN
                     SLYLAANGYDVDAWDKNAMSIANVERIKSIENLDNLHTRVVDLNNLTFDGQYDFILST
                     VVLMFLEAKTIPGLIANMQRCTKPGGYNLIVAAMDTADYPCTVGFTFAFKEGELRRYY
                     EGWERVKYNEDVGELHRTDANGNRIKLRFATMLARKK"
     gene            10361..11017
                     /locus_tag="OO357_003367"
     CDS             10361..11017
                     /locus_tag="OO357_003367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3313 domain-containing protein"
                     /protein_id="HCR5888200.1"
                     /translation="MRTTSFAKVAALCGLLALSGCASKITQPDKYNNYSDLKETTSAT
                     GKPVLRWVDPSFDQSKYDSIVWNPITYYPVPKPSTQVGQKVLDKILNYTNTEMKEAIA
                     QRKPVVTTAGPRSLIFRGAITGVDTSKEGLQFYEVVPVALVVAGTQMATGHRTMDTRL
                     YFEGELIDAATNKPVIKVVRQGEGKDLNNESTPMAFENIKQVIDDMATDATMFDVNKK
                     "
     gene            complement(11035..11478)
                     /gene="tnpA"
                     /locus_tag="OO357_003368"
                     /pseudo
     CDS             complement(11035..11478)
                     /gene="tnpA"
                     /locus_tag="OO357_003368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000604919.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
BASE COUNT         2967 a         2679 c         2883 g         2949 t
ORIGIN      
        1 aggggctatt ccatttcatc gtccaacaaa atgggtgcag tacagttgat agaaatatcg
       61 gtcggggcct tcgtctttct gattcccggt tagcctgaaa acagaaagtc tcaggcaccc
      121 gcaggcatcc tatgaggttt ccttagggac gaaaataatc acttcacgaa attgcgtgct
      181 gttttccaga atttttcgtc attcgggtta gccagtttag ccattcgtta ctctcttcat
      241 tccaatagca ttaattttct ctgcaataat tgttgtaaaa atgtgacgca aagaggtttt
      301 tggtcataag taattaccgt caagtgccga tgactttcta tcaggagtaa acctggacga
      361 gagacaacgg taatgaatac aactccctca cagcgattag gttttttgca tcacatcagg
      421 ctggttccgt tatttgcctg cattcttggc ggtatcttag ttctattcgc attaagttct
      481 gctctggctg gctatttcct ctggcaggcc gatcgcgatc agcgtgatgt tactgcggag
      541 attgagattc ggaccgggtt agcgaacagt tcagattttt tgcgttcagc ccggatcaat
      601 atgattcagg ccggggctgc gagtcgtatt gcggaaatgg aagcaatgaa gcgaaatatt
      661 gcgcaagccg aatcggagat taaacagtcg cagcaaggtt atcgtgctta tcagaatcga
      721 ccggtgaaaa cacctgctga tgaagccttc gacactgaat taaatcaacg ctttcaggct
      781 tatatcacgg gtatgcaacc tatgttgaaa tatgccaaaa atggcatgtt tgaagcgatt
      841 atcaatcatg aaagtgagca gatccgaccg ctggataatg cttataccga tattttgaac
      901 aaagccgtta agatacgtag caccagagcc aaccaactgg cggaactggc ccatcagcgc
      961 acccgcctgg gtgggatgtt catgattggc gcgtttgtgc ttgccctggt catgacgctg
     1021 ataacattta tggtgctacg tcggatcgtc attcgtccac tgcaacatgc cgcacaacgg
     1081 attgaaaaaa tcgccagtgg cgatctgacg atgaatgatg aaccggcggg tcgtaatgaa
     1141 atcggtcgct taagtcgtca tttacagcaa atgcagcatt cactggggat gacagtaggg
     1201 actgttcgac agggcgcgga agagatttat cgtggcacca gcgaaatttc agctggcaat
     1261 gcggacctgt catctcgcac cgaagaacaa gcggcggcta tcgaacaaac tgccgccagc
     1321 atggagcaac tcactgcgac ggtgaaacag aatgcggata acgcgcatca tgccagcaaa
     1381 ctggcgcaag aggcttctat taaagccagc gatggcgggc agacggtttc cggtgtagta
     1441 aaaacgatgg gcgctatctc cacgagttcg aagaaaattt ctgagatcac cgccgtcatc
     1501 aacagtattg ctttccagac gaatattctg gcactgaatg ctgccgttga agccgcgcga
     1561 gcgggtgagc aagggcgtgg atttgccgtt gtcgccagcg aagtacggac actcgcaagc
     1621 cgcagcgctc aggcagcgaa agagattgaa ggcttgatca gtgaatcagt caggttaatt
     1681 gacctggggt cggatgaggt ggcaacggca gggaaaacca tgagcactat tgttgatgcc
     1741 gtcgcgagtg tcacacatat catgcaggaa atcgccgccg cctcggatga acaaagtaga
     1801 ggcataactc aggttagcca ggcgatttct gaaatggata aggtgacgca acagaatgct
     1861 tctctggtag aagaggcctc agcagcggcg gtgtcccttg aagaacaggc tgcacgatta
     1921 actgaggcgg tgaacgtatt ccgtctgaac aaacattctg tgtcggcaga acctcgcgga
     1981 gcgggtgaac cagttagttt cgctacggtg tgaaaatgtt caaggaggga tcgacagatc
     2041 ccttcacctt tcagaacggc attgattttc gaatagcgtt aatcatcaac tggcaaccag
     2101 aagagaacgt cgcatctacg cgggtcagta ttccaatcgg ttcgcctgca ccatgtcccg
     2161 gaacaggcag ggccaccagc gtggcatgac gcaggtcgtc ttttacagcg ccagaaggga
     2221 caaaccacac gtaatcgtat tcaacggtaa gttgacgaga tagcgaagta gacagcgttt
     2281 cgatacaacc cgaaggaatt ttacagccct ggctctgcac taatgcatct gaatgctggc
     2341 gtggcgcagt gccttctggt gatacaacga ccggccattc cagcacccgg cttagcgtta
     2401 cgttctcctg aagtagcggg tgattagggc ggacaaccag cttcaacgat tcaagaaaca
     2461 gcagttcgta attaagcccg gtcatcagtt caggatctga catccgacca atgccgatat
     2521 cgatttcccc ggtttttaaa cccgccagaa tcatagggtt actcattgtc gcaacttgca
     2581 aggtcgtctc tttttgttgc tgatgaaact gacctataac cgaaggtaat atccccagtg
     2641 ccgcagtagg tagtgcacca accctgacga catcattatt aagaccttct ttacgatgaa
     2701 gcgactgtcc ggcagtgttg atggcgtcaa gaactctgac tgcatgcgtt aaaaattgtt
     2761 cgccgggtaa ggtaagttgc gccccctgac gaccacgctc aaacaagcga gcgcccgtca
     2821 gctgctccag ttcattcaat gtattagaga gcgcaggttg actcaaatta agggtttcag
     2881 ctgcgcgccc caaagttcct tgttgtgcga cagctacgaa tgtatgaagg tggcgcaaac
     2941 ggatgcgctg actaaacaga ctattttttt ccataagcga tgttaaaaac gaagcggtgt
     3001 cgctgacaag tgaagttgtt tgattatgat aacttgattg caaaatatta ttaacaatta
     3061 aagcaattat gttacagcaa aatggataat attgatgttt tcgcggcgag atcacagttt
     3121 gtaaattctt cccgccagag tgaatgcggt tacctacact ccagattact gaccactgga
     3181 ggcagacact atggcgaaca gcatcacggc ggatgagatt cgggaacagt tttcgcaggc
     3241 aatgtcagcc atgtaccagc aagaagttcc gcaatatggc acgctgctgg aactggtagc
     3301 tgatgtgaat ctggctgtgc tggaaaacaa tcctcaactg cacgaaaaaa tggtaaatgc
     3361 agacgagctg gcgcgactga atgttgaacg tcatggggcg attcgcgttg ggactgcaca
     3421 agagcttgct actcttcggc ggatgtttgc cattatgggg atgtacccgg tgagctatta
     3481 cgatctctcg caggcagggg tgccggtaca ttcgacagca tttcggccca ttgatgatgc
     3541 ttctctggcg cgtaatccct tccgcgtttt tacatcctta ctccgccttg agcttatcga
     3601 gaacgaaatt ttgcgccaga aagcggcgga gattctgcgt cagcgcgata tcttcacccc
     3661 acgttgtcga caactgttag aggaatatga gcagcagggc ggttttaacg aaacacaggc
     3721 acaggagttt gtgcaggaag ccctggaaac gtttcgctgg caccagttag caacggtaga
     3781 tgaagaaacc tatcgcgcct tgcacaacga acatcggttg attgctgatg tggtctgttt
     3841 tcctggatgc catatcaacc acctgacgcc acgtacgctg gatattgacc gggtgcagtc
     3901 gatgatgcct gaatgcggaa ttgaacccaa aattctcatc gaggggccgc cgcgccgcga
     3961 ggtaccgatt ttactacgcc agaccagctt taaagcactg gaagagacgg tgttgtttgc
     4021 ggggcagaaa cagggcacgc ataccgcgcg ctttggtgaa attgagcagc gtggcgtggc
     4081 attaacgccg aaagggcgac aactgtatga tgatcttctg cgtaacgctg gaaccgggca
     4141 ggataatctc actcaccaaa tgcatttaca ggaaaccttc cgcacttttc ctgacagtga
     4201 gtttttaatg cgtcagcaag ggttggcatg gttccggtac cgtctgacgc cttcgggtga
     4261 ggcgcatcgt caggcgattc atcctggaga cgatccacag cccttaattg aacgtggttg
     4321 ggtagtggcg caacccatca cctatgaaga tttcttgccc gttagcgcgg cggggatctt
     4381 ccagtcaaat ctgggtaatg aaacgcagac acgcagtcac ggtaatgcca gtcgcgaagc
     4441 atttgagcag gcgttgggtt gtccggtttt ggatgagttc cagctttacc aggaagcgga
     4501 agaacgcagt aaacgtcgct gtggtttgct ttaaattctg accatccgcc tttgcaaaaa
     4561 tttgcctgat tttacaaacg aatcaggctc atcccatcga cataaaaaaa atgccgattt
     4621 atgcatattc tctcagttca acaattggat tattaataaa tattgtctag agtgagcggt
     4681 cataaataag cactttcttg ccgctgaaaa cgaccagcgc gggaccattc acaacaccag
     4741 aaggactcac tttcaggtat ggatcgtaga cgatttatta aaggttcaat ggctatggcc
     4801 gccgtgtgcg gtaccagcgg cattgcttct cttttttctc aggcagcatt cgcggcagat
     4861 tctgatattg ccgacgggca aacccagcgt tttgacttct ccattctaca gtcaatggcg
     4921 cacgacttag cgcaaacagc gtggcgtggt gcgcctcgtc cgttacctga cacgctggcg
     4981 acaatgacgc cgcaggctta taacagtatt caatacgacg ccgaaaaatc gctctggcat
     5041 aacgttgaga accgtcaact ggacgctcag ttcttccata tgggaatggg attccgtcgc
     5101 cgcgttcgta tgttttctgt agatccagca acacatctgg cgcgtgaaat tcactttcgc
     5161 ccggagttgt tcaaatacaa cgatgcaggt gttgatacca aacaattaga agggcaaagc
     5221 gatctcggct ttgccggttt tcgcgtgttt aaagcccccg aactggcgcg ccgtgatgta
     5281 gtatcatttc tcggcgcgag ttatttccgc gccgttgatg atacatatca atacggtttg
     5341 tcggcccgcg gcctggcgat cgacacttac accgacagta aagaagagtt ccccgacttt
     5401 accgccttct ggtttgatac ggtaaaaccg ggggcaacta cctttaccgt ttatgcgttg
     5461 ctcgatagcg ccagcattac tggtgcctat aagttcacta tccattgtga gaaaagtcag
     5521 gtgattatgg atgtggaaaa tcacctgtat gcgcgcaaag acattaaaca gctgggcatt
     5581 tcgccgatga ccagtatgtt cagctgcggt actaatgaac gtcggatgtg cgatacaatt
     5641 catccgcaaa ttcatgactc tgatcgtctg tccatgtggc ggggcaacgg cgagtggatt
     5701 tgccgtccgc tgaataatcc gcaaaaattg cagttcaatg cttacaccga caacaacccg
     5761 aaagggtttg gtttattgca actggatcgt gacttctccc attatcagga cattatgggc
     5821 tggtataaca aacgcccaag tctgtgggtg gaaccgcgta acaagtgggg taagggcacc
     5881 atcggcctga tggaaatccc aacaacgggc gaaacgctgg ataacattgt ctgcttctgg
     5941 cagccagaaa aagctgtaaa agcaggtgat gagtttgcat tccagtatcg tctgtactgg
     6001 agtgcgcaac cgcctgttca ttgcccatta gcgcgcgtta tggcgacgcg taccggcatg
     6061 ggcggtttcc cggaaggttg ggcgccaggt gaacactatc ccgaaaaatg ggcgcgtcgt
     6121 tttgccgtcg atttcgttgg tggtgatctg aaagctgccg cgccaaaagg cattgagccg
     6181 gtgattacgc tttccagtgg ggaagcgaag caaatcgaaa ttctctatat tgaacccatc
     6241 gatggttatc gtattcagtt tgactggtat ccgacttcgg actccactga tccggtcgat
     6301 atgcggatgt atctacgttg tcagggggac gctatcagtg aaacatggct gtatcagtat
     6361 ttcccgccag cgccggataa acgtcagtat gttgacgacc gcgtgatgag ttaatcgttt
     6421 tttcttcggc accttcttcg ggaggtgccg tctggttaaa cacgatcccg ctcgcatttt
     6481 tccctaagtt aaatgagtaa tctgatggtg tgtatttcag atacaccgtg tcagccacta
     6541 acagggagtg cgtatgtttc cagaataccg agatttaata tcccgtctga aaaacgaaaa
     6601 tcctcgcttt atgtccttgt tcgataaaca caataaactt gatcatgaaa ttgccagaaa
     6661 ggaaggttcc gacggtcgag ggtacaatgc ggaagtggtc cgcatgaaaa aacaaaagct
     6721 acagttaaaa gatgagatgc tcaaaatcct gcagcaggag agcgtcaaag aggtgtaaac
     6781 tttcctaagc cgccagccag gcggcttttt taacaacagc atggattgac tggagataag
     6841 atgactgaaa cgataaaagt aagcgaatca cttgaattac atgctgttgc ggaaagtcac
     6901 gtcacacctc tttatcagtt aatctgtaaa aataaaacct ggttacagca gtcgctaaac
     6961 tggccgcagt tcgttcagag tgaagaggac acgcgaaaaa cggtgcaggg taatgtgatg
     7021 ttgcatcaac gcggctatgc caaaatgttc atgattttca aagaagatga acttatcggc
     7081 gttatctcgt ttaatcgtat tgaaccactg aataaaactg ctgaaatcgg ctactggctg
     7141 gacgaatctc atcaggggca ggggatcatt tctcaggcgc tgcagacatt gattcatcat
     7201 tacgctcagt ctggtgaact tagacgcttc gtgatcaaat gtcgggtgga caatccgcaa
     7261 agcaaccagg tcgctttgcg caatggtttt atccttgaag gttgcctgaa acaggctgag
     7321 ttcctgaatg atgcctatga tgatgtgaac ttatacgcgc gtattatcga ttcataataa
     7381 ccctgccagc ggcgttcgcg taatgcgctc ctcgccgtta atgacttttg gaccacggac
     7441 atgaaccgtg tcgccatcaa aggcttcaac gacggcgtgg tcggttaact ccacatggtt
     7501 ttcaattatc accgcacctg taatacggct ctcaccttgt attacaacgt gctcgtcgag
     7561 cagaatcggt ccaccacgta ccacggcatt tccgccaatc aagacatgat gttttaacac
     7621 acaattacct tccacaatgg catattccgc cacctgcgaa ctgtaatgaa tcgtcggaat
     7681 ggcatcttct tctatgccag ctttcacctg cgcgtgaccg tagactttag cgcaatcgca
     7741 tagccagacg ttgttctctt cattaccttc aacactggca aaatcaaaaa cttcggcgcg
     7801 atgttcaata aaagcatacc ggacgacggc atcgccataa atttgtgcct ggtggacaat
     7861 acgcgaggcg ctaacccttg cgcgatcata aatttggagc aggagttgat ggtcgggcgt
     7921 taagccttgt gcggcgacga tcatagaatg ttgatcaatt aaggcgtgac caaatattct
     7981 acattgcacg taaactaatg agtcatgaat ggtgacactg tcacttatat atgcaccctg
     8041 gctgatttca ctattatcga tccagacgtt atccgttgcg taaacctctc cccacagcac
     8101 gctggtgccg gtgatacgcg tattaccgga aatcaccgtt ccgccgaacg caatagcatt
     8161 ctggtcgtaa atccagcaat ttccttcctg tgctaacacc gtttcgcgat cgatccagcc
     8221 gccagcggtt cctgctatca catcgttaaa atcgctaatg gcgataatct gccgtaataa
     8281 cacacttttt ttagtgccat cttcctgata actaaaggct cgctgttctt cacttaggcg
     8341 atatttgcgc atagcgtttt cccacaggtg acttactata accgtagcaa attctgcggc
     8401 tctggctatg ctcgagaaaa ttccataaaa tgcatttcaa atatacttta taaattaaac
     8461 aaaatgagta agaagatgca gagcgataaa gtgctcaatt tgccggcagg ctactttggt
     8521 attgtgttgg ggacgatagg gatgggattt gcctggcgct atgccagcca ggtttggcag
     8581 gtcagccact ggttagggga tgggctggtg attctggcga tgatcatctg gggattattg
     8641 actagcgcat ttattacccg actcatacgc tttccgcata gcgtgctggc ggaagttcgc
     8701 catccagtgc tgagcagttt tgtgagtttg tttccggcaa cgacgatgct ggtggcgatt
     8761 ggttttgttc cgtggtttcg cccactggcg gtgtgcatgt tcagttttgg tgtcgtggtt
     8821 cagttggctt atgccgcctg gcaaactgcg ggattatggc gcggatctca ccctgaagaa
     8881 gctaccacgc ctggactgta tctgccgaca gttgccaaca actttatcag cgcaatggcc
     8941 tgtggtgcgt tgggctacac cgacgccggt ctggtgtttt taggcgcagg cgttttctca
     9001 tggctaagcc ttgaaccggt gatcttgcag cgtctgcgca gttcgggaga attacccacg
     9061 gcactgcgga catcactcgg cattcagctc gctcctgcgc tggtggcctg tagtgcctgg
     9121 ctgagcgtca acggcggcga gggtgacacg ctggcgaaaa tgcttttcgg ttatggactg
     9181 ctgcaactgc tgtttatgct acgtctgatg ccatggtatc tctcccagcc atttaatgct
     9241 tcattctgga gtttctcgtt cggcgtatct gcactggcaa ccaccggttt gcatctgggg
     9301 agtggcagcg ataatggatt tttccatacg ctggcggtgc cgctgtttat ctttaccaat
     9361 tttattattg caatactgct catccgtact tttgcgcttc tgatgcaggg aaaattgtta
     9421 gtcagaaccg agcgcgccgt tttaatgaaa gcagaggaca aagaatgatc attcgtgacg
     9481 aaaactattt tactgataaa tatgaattaa cccgcacaca ctctgaagta ctggaagcgg
     9541 tgaaagtggt taaaccgggt aaaacgctgg atctgggctg tggcaatggt cgtaacagtc
     9601 tttacctggc agccaatggt tatgatgttg acgcatggga taaaaatgcc atgagtatcg
     9661 ccaacgtcga gcgcattaaa tccattgaaa atctggataa tttacacacc cgggtcgttg
     9721 atctgaataa cctcacattt gatggacagt acgattttat tctttcgact gtggtgctga
     9781 tgttccttga ggctaaaacc atcccagggt tgattgccaa tatgcaacgt tgcactaaac
     9841 ctggtggtta caacctgatt gtggcggcga tggataccgc tgattatcca tgtaccgtcg
     9901 gcttcacgtt tgccttcaaa gagggagaat tacgtcgata ttacgaaggc tgggagaggg
     9961 tgaaatacaa tgaagacgtc ggcgagctgc accgcaccga cgccaacggt aatcgtatta
    10021 aactgcgttt cgccacgatg ctggcacgta aaaaatgacc cggtaagcac aaaacgcgtg
    10081 aaaattcccc acgctgagat gatttactgt tcttcttttc ggtaagcata ttttttatcg
    10141 aagggatgtg aaattaatca cagtagtcga agttttttag cagcttaact tactgaaatt
    10201 taactactga tgattgactt agcccctttt tcggcattga ctatgtcgtc tgaaaagggg
    10261 ctgaaaaatt tattttcacc aacacttttt ttgccacaac acgaagcggc gctttttgct
    10321 ataacttaga aagtaatata atcatctcag gaaactattc atgcgtacca catcatttgc
    10381 gaaagttgca gctttatgcg gcttgttggc tctgtctggt tgtgcctcta aaatcaccca
    10441 gccagataaa tataacaatt actctgattt aaaagaaaca acctcagcaa caggtaaacc
    10501 tgttttacgt tgggtagacc cgagttttga tcaaagcaaa tatgacagca tcgtctggaa
    10561 cccaatcact tattatccgg taccaaaacc ttctacccag gtagggcaga aagttctgga
    10621 taaaattttg aactatacca acaccgaaat gaaagaagcg atagcgcagc gtaaaccagt
    10681 ggttaccacc gctgggccgc gtagtctgat tttccgtggg gccattaccg gtgtagatac
    10741 cagcaaagaa gggctgcaat tctatgaagt ggttcctgtt gcattagtgg ttgcggggac
    10801 gcaaatggct acaggccacc gtaccatgga tactcgcctc tattttgaag gtgagctgat
    10861 tgatgcagcg actaataaac cggttatcaa agtcgttcgt cagggcgaag gtaaagacct
    10921 gaataacgaa agtacgccaa tggctttcga aaatattaaa caagttattg atgacatggc
    10981 gaccgatgcc accatgtttg acgttaacaa aaagtagttc cagacgcgcc atcgttagat
    11041 ggcgcttttt atccggtgcg ccgtaaaacc ccatccttca gggcggggat ataaggcgcg
    11101 gttttccacc tgaccaggtg tttgctgttg ttcaatgtat tggcggatga cggatattgg
    11161 cgcacctccg cagctactgg caaaatagcc aggactccac aaaacgcctt tgtagtaata
    11221 cctgactgca atatctggtc gatctcgtcg cagtaaccta cccgatacgc ctttgaggct
    11281 gtttaccaga ctggatatcg ccagtttggg aggttagtta attaacaaat ggacgtgatc
    11341 tggttcgcca tccatttcaa ccagttcagc ttcaaaataa gcacatacat ttgaaaagta
    11401 agtgcgtagt ttttctgtcg cgtcatggtc aaaaatctgg cgtcggtatc tggtgacaaa
    11461 gactaggtga acatgcat
//
LOCUS       DAJTRC010000114        11186 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.114, whole genome shotgun sequence.
ACCESSION   DAJTRC010000114 DAJTRC010000000
VERSION     DAJTRC010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.114"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..611)
                     /gene="hdhA"
                     /locus_tag="OO357_003369"
     CDS             complement(<1..611)
                     /gene="hdhA"
                     /locus_tag="OO357_003369"
                     /EC_number="1.1.1.159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="7-alpha-hydroxysteroid dehydrogenase"
                     /protein_id="HCR5888201.1"
                     /translation="MFNSDNLRLDGKCAIITGAGAGIGKEIAITFATAGASVVVSDIN
                     ADAANHVVDEIQQLDGQAFAWRCDITSEQELSALADFAVSNLGKVDILVNNAGGGGPK
                     PFDMPMADFRRAYELNVFSFFHLSQLVAPEMEKNGGGVILTITSMAAENKNINMTSYA
                     SSKAAASHLVRNMAFDLGEKNIRVNSIAPGAILTDALKSVITP"
     gene            complement(723..1751)
                     /gene="malI"
                     /locus_tag="OO357_003370"
     CDS             complement(723..1751)
                     /gene="malI"
                     /locus_tag="OO357_003370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mal regulon transcriptional regulator MalI"
                     /protein_id="HCR5888202.1"
                     /translation="MATAKKITIHDVALAAGVSVSTVSLVLNGKGRISTATGERVNAA
                     IEELGFVRNRQASALRGGQSGVIGLIVRDLSAPFYAELTAGLTEALEAQGRMVFLLHG
                     GKDGEQLAQRFSLLLNQGVDGVVIAGAAGSSDDLRRMAEEKAIPVIFASRASYLDDVD
                     TVRPDNMQAAQLLTEHLIRNGHQRIAWLGGQSSSLTRAERVGGYCATLLKFGLPFHSD
                     WVLECTSSQKQAAEAITALLRHNPTISAVVCYNETIAMGAWFGLLKAGRQSGESGVDR
                     YFEQQVSLAAFTDATPTTLDDIPVTWASTPARELGITLADRMMQKITHEETHSRNLII
                     PARLIAAK"
     gene            1926..3518
                     /gene="malX"
                     /locus_tag="OO357_003371"
     CDS             1926..3518
                     /gene="malX"
                     /locus_tag="OO357_003371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707521.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS maltose transporter subunit IICB"
                     /protein_id="HCR5888203.1"
                     /translation="MTAKTAPKVTLWEFFQQLGKTFMLPVALLSFCGIMLGIGSSLSS
                     HDVITLIPVLGNPVLQAIFTWMSKIGSFAFSFLPVMFCIAIPLGLARENKGVAAFAGF
                     VGYAVMNLAVNFWLTNKGILPTTDAAVLKANNIQSILGIQSIDTGILGAVIAGIIVWM
                     LHERFHNIRLPDALAFFGGTRFVPIISSLVMGLVGLVIPLVWPIFAMGISGLGHMINS
                     AGDFGPMLFGTGERLLLPFGLHHILVALIRFTDAGGTQEVCGQTVSGALTIFQAQLSC
                     PTTHGFSESVTRFLSQGKMPAFLGGLPGAALAMYHCARPENRHKIKGLLISGLIACVV
                     GGTTEPLEFLFLFVAPVLYVIHALLTGLGFTVMSVLGVTIGNTDGNIIDFVVFGILHG
                     LSTKWYMVPVVAAIWFVVYYVIFRFAITRFNLKTPGRDSEVASSIEKAVAGAPGKSGY
                     NVPAILEALGGADNIVSLDNCITRLRLSVEDMSLVNVQALKDNRAIGVVQLNQHNLQV
                     VIGPQVQSVKDEMAGLMHTVQA"
     gene            3528..4700
                     /gene="malY"
                     /locus_tag="OO357_003372"
     CDS             3528..4700
                     /gene="malY"
                     /locus_tag="OO357_003372"
                     /EC_number="4.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional maltose regulon transcriptional
                     repressor/cystathionine beta-lyase MalY"
                     /protein_id="HCR5888204.1"
                     /translation="MFDFSKVVDRHGTWCTQWDYVADRFGTADLLPFTISDMDFATAP
                     CIIEALNQRLMHGVFGYSRWKNDEFLAAIAHWFSTQHYTAIDTQTVVYGPSVIYMVSE
                     LIRQWSETGEGVVIHTPAYDAFYKAIEGNQRTVMPVALEKQADGWFCDMGKLEAVLAK
                     PECKIMLLCNPQNPTGKVWTCDELEIMADLCERHDVRVISDEIHMDMVWGEQPHIPWS
                     NVARGDWALLTSGSKSFNIPALTGAYGIIENSSSRDAYLSALKGRDGLSSPSVLALTA
                     HIAAYQQGAPWLDALRVYLKDNLTYISDKMNAAFPELNWQIPQSTYLAWLDLRPLNID
                     DNALQKALIEQEKVAIMPGYTYGEEGRGFVRLNAGCPRSKLEKGVAGLINAIRAVR"
     gene            4804..5805
                     /gene="add"
                     /locus_tag="OO357_003373"
     CDS             4804..5805
                     /gene="add"
                     /locus_tag="OO357_003373"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HCR5888205.1"
                     /translation="MIDTTLPLTDIHRHLDGNIRPQTILELGRQYNISLPAQSLETLI
                     PHVQVIANEPDLVSFLTKLDWGVKVLASLDACRRVAFENIEDAARNGLRYVELRFSPG
                     YMAMAHKLPVAGVVEAVIDGVREGCRTFGVQAKLIGIMSRTFGEAACQQELEAFLAHR
                     DQITALDLAGDELGFPGSLFLSHFNRARDAGWHITVHAGEAAGPESIWQAIRELGAER
                     IGHGVKAIEDRALMDFLAEQQIGIESCLTSNIQTSTVAELAAHPLKTFLEHGIRASIN
                     TDDPGVQGVDIIHEYTVAAPAAGLSREQIRQAQINGLEMAFLSAEEKRALREKVAAK"
     gene            complement(5841..6881)
                     /locus_tag="OO357_003374"
     CDS             complement(5841..6881)
                     /locus_tag="OO357_003374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HCR5888206.1"
                     /translation="MSDNIRVGLIGYGYASKTFHAPLIAGTPGLELAVISSSDETKVK
                     ADWPTVTVVSEPKHLFNDPNIDLIVIPTPNDTHFPLAKAALEAGKHVVVDKPFTVTLS
                     QARELDALAKSLGRVLSVFHNRRWDSDFLTLKGLLAEGVLGEVAYFESHFDRFRPQVR
                     DRWREQGGPGSGIWYDLAPHLLDQAITLFGLPVSMTVDLAQLRPGAQSTDYFHAILSY
                     PQRRVILHGTMLAAAESARYIVHGSRGSYVKYGLDPQEERLKNGERLPQEDWGYDMRD
                     GVLTRVEGEERVEETLLTVPGNYPAYYAAIRDALNGDGENPVPASQAIQVMELIELGI
                     ESAKHRATLCLA"
     gene            7124..7249
                     /gene="blr"
                     /locus_tag="OO357_003375"
     CDS             7124..7249
                     /gene="blr"
                     /locus_tag="OO357_003375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_025303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division septum protein Blr"
                     /protein_id="HCR5888207.1"
                     /translation="MNRLIELTGWIVLVVSVILLGVASHIDNYQPPEQSASVQHK"
     gene            7522..7737
                     /gene="ydgT"
                     /locus_tag="OO357_003376"
     CDS             7522..7737
                     /gene="ydgT"
                     /locus_tag="OO357_003376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription modulator YdgT"
                     /protein_id="HCR5888208.1"
                     /translation="MTVQDYLLKFRKISSLESLEKLYDHLNYTLTDDQELINMYRAAD
                     HRRAELVSGGRLFDLGQVPKSVWHYVQ"
     gene            7823..8263
                     /locus_tag="OO357_003377"
     CDS             7823..8263
                     /locus_tag="OO357_003377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707526.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2569 domain-containing protein"
                     /protein_id="HCR5888209.1"
                     /translation="MTTTTPQRIGGWLLGPLAWLLVALLSTTLALLLYTAALSSPQTF
                     QTLGGQALTTQILWGVSFITAIAMWYYTLWLTIAFFKRRRCVPKHYIIWLLISVLLAV
                     KAFAFSPVEVGIAVRQLLFTLLATALIVPYFKRSSRVKATFVNP"
     gene            8340..8921
                     /gene="rsxA"
                     /locus_tag="OO357_003378"
     CDS             8340..8921
                     /gene="rsxA"
                     /locus_tag="OO357_003378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017456621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxA"
                     /protein_id="HCR5888210.1"
                     /translation="MTDYLLLFVGTVLVNNFVLVKFLGLCPFMGVSKKLETAMGMGLA
                     TTFVMTLTSICAWLIDTWILIPLNLIYLRTLAFILVIAVVVQFTEMVVRKTSPVLYRL
                     LGIFLPLITTNCAVLGVALLNINLGHNFLQSALYGFSAAVGFSLVMVLFAAIRERLAV
                     ADVPAPFRGNAIALITAGLMSLAFMGFSGLVKL"
     gene            8921..9499
                     /gene="rsxB"
                     /locus_tag="OO357_003379"
     CDS             8921..9499
                     /gene="rsxB"
                     /locus_tag="OO357_003379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxB"
                     /protein_id="HCR5888211.1"
                     /translation="MNAIWIAVAAVSLLGLAFGAILGYASRRFAVEDDPVVEKIDEIL
                     PQSQCGQCGYPGCRPYAEAISCNGEKINRCAPGGEAVMLKIAELLNVEPQPLDGEAQE
                     LTPARMVAVIDENNCIGCTKCIQACPVDAIVGATRVMHTVMSDLCTGCNLCVDPCPTH
                     CISLQPVAETPDSWKWDLNTIPVRIIPVEHHA"
     gene            9492..>11186
                     /gene="rsxC"
                     /locus_tag="OO357_003380"
     CDS             9492..>11186
                     /gene="rsxC"
                     /locus_tag="OO357_003380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxC"
                     /protein_id="HCR5888212.1"
                     /translation="MLKLFSAFRKNKIWDFNGGIHPPEMKTQTNGTPLRQVLLAQHFV
                     IPLKQHIGAEGELCVSVGDKVLRGQPLTRGRGKMLPVHAPTSGTVTAIAPHSTAHPSA
                     LAELSVIIDADGEDCWIPRDGWVDYRSRSREELIERIHQFGVAGLGGAGFPTGVKLQG
                     GGDKIETLIINAAECEPYITADDRLMQDCAAQVVEGIRILAHILQPREILIGIEDNKP
                     QAISMLRAVLADSNDISLRVIPTKYPSGGAKQLTYILTGKQVPHGGRSSDIGVLMQNV
                     GTAYAVKRAVIDGEPITERVVTLTGEAIARPGNVWARLGTPVRHLLNDAGFCPSADQM
                     VIMGGPLMGFTLPWLDVPVVKITNCLLAPSANELGEPQEEQSCIRCSACADACPVDLL
                     PQQLYWFSKGQQHDKATTHNIADCIECGACAWVCPSNIPLVQYFRQEKAEIAAIRQEE
                     KRAAEAKARFEARQARLEREKATRLERHKSAAVQPAAKDKDAIAAALARVKEKQAQAT
                     QPIVIKAGERPDNSAIIAEREARKAQARAKQAELEQTNDAATVAEPRKTAVEAAIAR"
BASE COUNT         2598 a         2878 c         2817 g         2893 t
ORIGIN      
        1 tctggtgtaa taacggattt cagggcatcg gttaatattg cccccggcgc aatgctattc
       61 acccgaatat ttttttcacc caggtcaaat gccatatttc tgaccagatg actggccgca
      121 gctttagatg atgcatagga agtcatgttt atatttttat tttctgccgc catagaagtg
      181 atggtcagaa taacgccacc tccatttttt tccatttctg gcgcaacaag ttgtgacaga
      241 tggaaaaaag aaaacacatt cagttcataa gcacggcgaa aatctgccat tggcatatca
      301 aacggtttag gaccaccgcc accggcgttg ttaaccagga tatcaacttt acccagatta
      361 ctgacggcaa agtctgccag tgcagagagt tcctgttcgg aagtaatatc acaacgccag
      421 gcaaatgcct gaccatccag ttgttgaatt tcgtctacaa catggttagc tgcgtcggcg
      481 ttaatatcac tgaccaccac agatgcgcca gctgtcgcga atgtaatggc gatttcttta
      541 ccaatacctg cacccgcacc tgtgatgatg gcgcattttc cgtcgagtct caggttgtca
      601 gaattaaaca cataaacctc ctgtgaactt cagtaaaaat aaaaggcacc gcctgtgtgc
      661 cacatttttt atcattaacc ttgaggaaag gacactacca agtatagcag ataaagagag
      721 gattatttcg ccgcaatgag ccgggcggga ataataagat tgcgtgaatg cgtctcttca
      781 tgggtgattt tttgcatcat gcgatccgca agtgtgatac caagttcccg cgctggcgtg
      841 ctggcccagg taacaggtat atcatcaagt gtggttggtg tcgcatcggt aaatgccgcc
      901 agcgaaacct gttgctcaaa gtaacgatcg actccgcttt ccccgctttg ccgccctgct
      961 ttcagcaaac caaaccatgc ccccatcgca atagtttcgt tatagcaaac cacggcactg
     1021 atggtcgggt tatgacgtaa aagcgccgtg atagcttccg cggcttgctt ctggctggaa
     1081 gtgcactcca acacccaatc gctgtgaaac ggcaggccaa attttagtag agttgcacaa
     1141 tagcccccca cccgttctgc acgggttaat gaggaacttt gccctcccag ccaggcgatc
     1201 cgctgatgcc cattgcgaat gagatgctcc gtcaacaact gtgcagcctg catgttgtcc
     1261 gggcgaaccg tatcaacatc atcaagataa ctggcacggg aagcgaaaat caccgggata
     1321 gctttttctt ctgccatccg ccgcaggtca tcgctgcttc ctgcagcccc ggcaattacc
     1381 acaccatcga caccctgatt cagtaacagt gaaaaccgct gtgccagctg ctcgccgtct
     1441 ttaccgccgt gaagcaaaaa aaccatccgt ccctgtgctt ccagagcttc cgtcaatccg
     1501 gccgtcaatt cggcgtaaaa cggcgcagat aaatcacgga cgatcaaacc aatgacgccg
     1561 ctttgcccgc cgcgcagcgc cgacgcctgg cgattgcgca caaatcccag ctcttcaatg
     1621 gcggcgttaa cgcgttctcc tgtggcggta gagattcgcc ctttgccatt aagcaccagc
     1681 gaaacggtac ttaccgacac gcccgcagcc agcgcaacat catgaatggt tatttttttg
     1741 gcggtagcca tgcagatgac ctactccctg attatgtgac agataaaacg ttttaccttt
     1801 tatttatctt atacccgcta ttatcgttgc gtaatgtgat ttatgactca ctaaaatttg
     1861 ataaaacgtt ttatcttctc gcgcaattta ctgaatccag attgttctct acgaggagtc
     1921 gttttatgac ggcgaaaaca gcaccgaaag tcacgctgtg ggagttcttc cagcagttag
     1981 gcaaaacctt catgttaccc gtggcattat tgtcgttctg cggcattatg ctcggcattg
     2041 gtagttctct tagcagccat gatgtcataa ccctgatccc ggtcctgggc aaccccgtgt
     2101 tgcaggctat ctttacctgg atgagtaaga ttggctcgtt tgcttttagt ttcctgcctg
     2161 tcatgttctg tatcgccatc ccgctgggcc tggcacgcga aaataaaggc gtagcggcat
     2221 tcgctggctt cgtcggttat gcggtaatga acctcgcggt aaacttctgg ttgaccaata
     2281 aaggcattct gccaaccaca gatgccgccg ttctgaaagc caataacatc cagagcattc
     2341 ttgggatcca gtcgatcgat accgggatcc tcggtgcggt gatcgccggt attatcgtct
     2401 ggatgctgca tgagcgtttc cataatatcc gcctgccgga tgcgctggca ttcttcggcg
     2461 gtacgcgctt cgtaccaatt atctcctcgc tggtgatggg ccttgtcggc ctggtgattc
     2521 cattagtctg gccgattttc gccatgggta ttagcggctt gggccatatg ataaacagcg
     2581 cgggtgattt cggaccgatg ctgtttggta ccggtgaacg tctgctgttg ccgtttggtc
     2641 tgcatcacat tctggtggca ttaattcgct ttaccgacgc aggcggcacg caggaggtct
     2701 gcggtcaaac cgtcagcggc gcactgacca tcttccaggc gcaattgagt tgcccgacca
     2761 ctcacggttt ttctgaaagc gtcacgcgtt tcctttcgca aggtaaaatg cctgcgtttc
     2821 tcggcggtct accaggtgca gcgttagcta tgtatcactg cgcgcgcccg gaaaatcgcc
     2881 ataaaattaa aggtctgctg atttctggcc tgatcgcctg cgtcgttggc ggcactaccg
     2941 aaccgctgga attcctgttc ctgttcgtag cgccagttct gtatgtcatc cacgcgctgt
     3001 taaccggcct cggcttcacc gtcatgtctg tgctcggcgt caccatcggt aataccgacg
     3061 gcaatatcat cgacttcgtg gtgttcggta ttttgcatgg tctgtcaacc aagtggtaca
     3121 tggtgccagt ggtggcggca atctggtttg tcgtttacta cgtcatcttc cgtttcgcta
     3181 tcacccgctt caatctgaaa accccggggc gcgatagcga agttgccagc tcaatcgaaa
     3241 aagccgttgc cggtgcgccg ggtaaatcag gttacaacgt tcctgcaatc ctcgaagcat
     3301 taggcggtgc cgacaatatt gtcagcctcg ataactgcat tacccgtctg cgtttgtctg
     3361 tggaagatat gtcgcttgtt aatgtgcagg cactgaagga caatcgggca attggcgtag
     3421 tacaacttaa tcaacataac ctgcaggttg ttatcgggcc acaagttcag tcagtaaaag
     3481 atgaaatggc cggtctgatg catactgtcc aggcataagg ataagatatg ttcgattttt
     3541 caaaggtcgt ggatcgtcat ggcacatggt gtacacagtg ggattatgtc gctgaccgtt
     3601 tcggcactgc tgacctgtta ccgttcacga tttcagacat ggattttgcc actgccccct
     3661 gcattatcga ggcgctgaat cagcgcctga tgcacggcgt atttggctac agccgctgga
     3721 aaaacgatga gtttctcgcg gctattgccc actggttttc cacccagcat tacaccgcaa
     3781 ttgatacgca gacggtggtg tatggccctt ctgtcatcta tatggtttca gaactgattc
     3841 gtcagtggtc tgaaacaggt gaaggcgtgg tgatccacac gcccgcctat gacgcatttt
     3901 acaaggccat tgaaggtaac cagcgcacag taatgcccgt tgctttagag aagcaggctg
     3961 acggctggtt ttgcgatatg ggcaagctgg aagccgtttt ggcgaaacca gaatgtaaaa
     4021 ttatgctcct gtgtaaccca cagaatccta ccgggaaagt gtggacgtgc gatgagctgg
     4081 agatcatggc tgacctgtgc gagcgtcatg atgtgcgggt tatttccgat gaaatccata
     4141 tggatatggt ctggggcgag cagccgcata ttccctggag taatgtggcg cggggagact
     4201 gggcgttgct gacatcggga tcgaaaagtt tcaatattcc cgcgctgacc ggtgcttacg
     4261 ggattataga aaatagcagc agccgtgatg cctatttatc ggcactgaaa ggacgtgatg
     4321 ggctttcttc cccttcggta ctggcgttaa ctgcccatat cgccgcctat cagcaaggtg
     4381 caccgtggct ggatgcctta cgcgtctatc tgaaagataa cctgacgtat atctcagata
     4441 aaatgaacgc cgcgtttcct gaactcaact ggcagatccc gcaatccact tatctggcct
     4501 ggcttgatct acgtccgttg aatattgacg acaacgcgtt gcaaaaagcg cttatcgagc
     4561 aagaaaaagt cgcgatcatg ccggggtata cctatggtga agaaggtcgt ggttttgtcc
     4621 gtctcaatgc cggctgccca cgttcgaaac tggaaaaagg tgtggctgga ttaataaacg
     4681 ccatccgcgc tgttcgctaa ccccaattgc gcaacgtaaa aaatcgttgc gcaatcgtgg
     4741 atttttaccc tgctttgttt ttataatggt gcgcactttt atatccagaa aaagagtgcg
     4801 accatgattg ataccaccct gccattaact gatatccatc gccaccttga tggcaacatt
     4861 cgtccccaga ccattcttga acttggccgc cagtataata tctcgcttcc tgcacaatcc
     4921 cttgaaacac tgattcccca cgttcaggtc attgccaacg aacccgatct ggtgagcttt
     4981 ctgaccaaac ttgactgggg cgttaaagtt ctcgcctctc ttgatgcctg ccgccgcgtg
     5041 gcatttgaaa acattgaaga tgcagcccgt aacggcctgc gctatgtcga gctgcgtttt
     5101 tcaccaggct acatggcaat ggcacataag ctgcctgtag cgggtgttgt cgaagcggtg
     5161 atcgatggcg tacgtgaagg ttgccgcacc tttggtgtgc aggcgaagct tatcggcatt
     5221 atgagccgaa ccttcggcga agccgcctgt cagcaagagc tggaggcctt tttagcccac
     5281 cgtgaccaga ttaccgcact tgatttagct ggtgatgaac ttggattccc gggaagtctg
     5341 ttcctttctc acttcaaccg cgcgcgtgat gcgggctggc atattaccgt ccatgcaggc
     5401 gaagctgccg ggccggaaag catctggcag gcgattcgtg aactgggtgc ggagcgtatt
     5461 ggacatggcg taaaagccat tgaagatcgg gcgctgatgg attttctcgc cgagcaacaa
     5521 attggtattg aatcctgtct gacctccaat attcagacca gcaccgtagc agagctggct
     5581 gcacatccgc tgaaaacgtt ccttgagcat ggcattcgtg ccagcattaa cactgacgat
     5641 cccggcgtac agggagtgga tatcattcac gaatataccg ttgccgcgcc agctgctggg
     5701 ttatcccgtg agcaaatccg ccaggcacag attaatggtc tggaaatggc tttcctcagc
     5761 gcagaggaaa aacgcgcact gcgagaaaaa gtcgccgcga agtaacaaaa atggatggtg
     5821 caaattgcac catccatttt tcacgcaagg cacaacgtcg cgcgatgttt ggcggattcg
     5881 atgcccagct caatcaactc cattacctgg attgcctggc ttgccggaac cggattttca
     5941 ccatcgccat ttaacgcatc acgaatcgcc gcatagtaag ccggataatt cccaggcacc
     6001 gtcaacagcg tttcttcgac acgttcctca ccttccacgc gggtaagtac gccatcacgc
     6061 atatcgtagc cccagtcttc ctgcggcaga cgctcgccat ttttcagacg ttcttcctgt
     6121 ggatcgaggc catatttcac ataactgcct cgggatccat gcacgatata ccgtgctgac
     6181 tcagcggctg ccagcatggt accgtgtaaa atgactcgcc gctgtggata ggacaagatg
     6241 gcgtggaaat aatcggtcga ctgcgctccg ggccgtaact gtgccaaatc taccgtcatg
     6301 ctgaccggta aaccaaatag cgtaatggcc tgatcaagaa gatgcggtgc taaatcgtac
     6361 cagataccgc tgcctgggcc gccctgttca cgccaacgat cgcgcacctg cggacggaag
     6421 cggtcaaaat gagactcaaa gtaagcaact tcacccagca cgccttccgc gagcaaacct
     6481 tttagcgtca agaaatcgct atcccaacgg cggttatgga atacagacag cacacgcccc
     6541 aggctttttg ccagcgcatc cagctctcgc gcttgtgaca gtgtcacggt aaagggttta
     6601 tcaacgacca catgtttacc cgcctcaagc gccgctttgg ctaacgggaa atgggtatcg
     6661 ttgggtgtag gaatgacaat caggtctatg ttgggatcgt taaacagatg cttcggctca
     6721 gagacaaccg taaccgttgg ccagtcggct tttacttttg tttcatcact gctggagatt
     6781 accgccagtt ccagcccggg cgtgcccgca atcaggggcg catggaaggt tttgctcgca
     6841 taaccatacc caatcaaccc aacacggatg ttgtcgctca tgtcattgcc tctcattgaa
     6901 gtatgatggc tatttgacac tatcctttac ccacgctcaa cagtttaata acctgccagc
     6961 aataagggat gttgtttaac ttaagtcaaa aaaatagcga attttccaac gacaaaagct
     7021 aaatatcgca aaaacctcag taaaaatctt gctggagcta ttattgctaa gtaacattta
     7081 ccccctgaag ttaatggatc aatcaagaga gatgtgggct gtaatgaatc gtcttattga
     7141 attaacaggt tggatcgttc ttgtcgtttc agtcattctt cttggcgtgg cgagtcacat
     7201 tgacaactat cagccacctg aacagagtgc ttcggtacaa cacaagtaag ctctgcactt
     7261 gtggagcgac atgctgcccg tccgggtgca tgttttcact tgtcggatat taaaccagga
     7321 atttattatc ttgttcgatg ttgttggtga ttgtcaggga tagtaaagcg cgttactctt
     7381 ttcagagcag tttttcatcc ctgaatattt gccatcagcg cattcttatt attgtaataa
     7441 cacaagcgtt cacttttggt gaaattttat catcactaat aatacctacc cctttggtat
     7501 taattatttt atggaccttt tatgactgtt caggactact tattaaaatt tcgcaaaatc
     7561 agttcactcg aaagtctgga aaaactctac gaccatctta attacaccct gacggacgat
     7621 caggaactga tcaatatgta tcgtgctgcc gatcaccgtc gcgcagagct ggtttctggc
     7681 gggcgtttgt ttgacctcgg ccaggtaccg aagtccgtct ggcactatgt ccaataaaga
     7741 aagtagcgat cggttataaa agccttataa taacgcccct aaaattagat ttgcctgttt
     7801 gcgtcgctat tctggagaac ctatgaccac cacaacgcca caacgtattg gaggctggtt
     7861 gcttggcccg ttggcctggc ttttagtcgc tctgttgagt acgacgctgg cgttgttgct
     7921 gtataccgct gcgttatctt ctccgcaaac atttcaaacg ctcggcggac aagcactgac
     7981 gacacaaatc ttatggggcg tttcttttat taccgctatc gccatgtggt attacacact
     8041 gtggttgacc atcgctttct ttaaacgtcg gcgttgtgtt cctaaacact atattatctg
     8101 gctgcttatt tccgtattgc tggcagtaaa agcattcgct ttttcacccg tggaagttgg
     8161 catagccgtt cgccagttgc tgtttacttt gttggcaacc gcattgatcg tcccctattt
     8221 caaacgctcg tcgcgggtaa aagcgacgtt tgtgaatccg taataacctt acagttaacc
     8281 tgttgtcgcc tgctctggat taacggataa taggcggctt ttttatttca ggccgaaaaa
     8341 tgactgacta cctgttactg tttgtcggaa ctgtactggt caataacttt gtactggtca
     8401 agtttctcgg tctctgtccg tttatggggg tttccaaaaa actggaaacc gcgatgggca
     8461 tggggctggc aacaacgttt gtgatgacgc tgacgtctat ttgcgcctgg cttatcgata
     8521 cgtggatttt gatcccactt aatctgattt acctgcgcac cctggcattt attctggtga
     8581 ttgctgtggt cgtgcagttc accgagatgg tggtgcgcaa aaccagcccg gtgctttacc
     8641 gtctgctggg gatttttttg ccgcttatca ccaccaactg tgcagtgctc ggcgtggcgt
     8701 tgctgaatat caatctcggg cacaatttct tgcagtcggc gctgtacggt ttttccgccg
     8761 ctgtcggttt ctcgctggtg atggtgctct tcgccgccat ccgcgaacgc cttgctgtgg
     8821 ctgatgtccc ggcacctttt cgcggtaatg ccattgcgtt aattaccgca ggtcttatgt
     8881 ctctggcctt tatgggcttt agtggtttgg tgaagttgta atgaatgcta tctggattgc
     8941 cgttgccgcc gtgagcctgc tgggcctggc gtttggcgcc attctgggtt atgcctcccg
     9001 ccgttttgcg gtggaagacg atccggtcgt tgagaaaatt gacgaaatct taccgcagag
     9061 ccagtgtggt cagtgcggtt atcccggctg tcgcccctac gcggaagcca tcagctgtaa
     9121 cggtgaaaaa atcaaccgtt gcgccccagg tggcgaagct gtgatgctaa aaattgccga
     9181 gttgcttaat gtcgagccgc agccgctgga tggcgaagcg caagagctaa cgcctgcgcg
     9241 gatggtggcg gttattgatg aaaataactg tattggctgc actaaatgta ttcaggcttg
     9301 tccggtagac gccatcgttg gcgctacccg tgtcatgcat acggtaatga gtgatctctg
     9361 tacgggctgc aatttatgtg ttgatccgtg cccgacgcac tgcatctcgt tgcaaccggt
     9421 cgcagaaaca cctgactcct ggaaatggga tctgaacacc attcccgtgc gtatcattcc
     9481 cgtggaacac catgcttaag ttattctctg cattcagaaa aaataaaatc tgggatttca
     9541 acggcggcat ccatccaccg gagatgaaaa cccagaccaa cggtaccccc ctgcgccagg
     9601 tactactggc gcagcatttt gttattccac tgaaacagca tattggcgct gaaggtgagt
     9661 tgtgcgttag cgtcggcgat aaagtattgc gcggccagcc gcttacccgt ggtcgcggca
     9721 aaatgctgcc tgttcacgcg cccacctcgg gtaccgttac ggctattgcg ccccactcta
     9781 cggctcatcc ttcagcttta gctgaattaa gcgtgattat tgatgccgat ggtgaagact
     9841 gctggatccc gcgcgacggc tgggtcgatt atcgctctcg cagtcgcgaa gagttaatcg
     9901 agcgcataca tcagtttggt gttgccgggc tgggcggtgc cggattcccg acaggcgtta
     9961 aattgcaggg tggtggagat aagattgaaa cgttgattat caacgctgct gagtgcgagc
    10021 cgtacattac cgccgatgac cgtttgatgc aggattgcgc ggctcaggtc gtagagggta
    10081 ttcgcattct tgcgcatatt ctgcagccac gcgaaattct tatcggcatt gaagataaca
    10141 aaccgcaggc gatttccatg ctgcgcgcgg tgctggcgga ctctaacgat atttctctgc
    10201 gggtgattcc aaccaaatat ccttctggcg gtgctaaaca attaacctac attctgaccg
    10261 ggaagcaggt tccacatggc gggcgttcat ccgatatcgg cgtattaatg caaaacgtcg
    10321 gcactgctta tgcagtgaaa cgtgccgtta ttgatggcga gccgattacc gagcgtgttg
    10381 taaccctgac tggcgaagca atcgctcgcc cgggcaacgt ctgggcacgg ctggggacgc
    10441 cagtgcgtca tttattgaat gatgccggat tctgcccctc tgccgatcaa atggtgatta
    10501 tgggtggccc gctaatgggc tttaccttgc catggctgga tgtcccggtc gtaaagatta
    10561 ccaactgtct gttggctccc tctgccaatg aacttggcga accacaggaa gaacaaagct
    10621 gcatccggtg tagcgcctgt gctgacgcct gccctgtgga tcttttgccg caacagttgt
    10681 actggttcag caaaggtcag caacacgata aagctaccac gcataacatt gccgactgca
    10741 ttgaatgtgg ggcttgcgcg tgggtttgcc cgagcaatat tcccttggtg caatatttcc
    10801 gtcaggaaaa agctgaaatt gcggctattc gtcaggaaga aaagcgcgcc gcagaagcca
    10861 aagcgcgttt cgaagcgcgc caggctcgtc tggagcgcga aaaagcgact cgccttgaac
    10921 gacataagag cgcagccgtt caacctgcag ccaaagataa agatgcgatt gctgccgctc
    10981 tggcgcgggt gaaagagaaa caggcccagg ctacacagcc tattgtgatt aaagcgggcg
    11041 aacgcccgga taacagtgca attattgcag aacgggaagc ccgtaaagcg caagccagag
    11101 cgaaacaggc agaactggag caaactaacg acgcagcaac cgttgctgaa ccacgtaaaa
    11161 ctgccgttga agcagctatc gcccgc
//
LOCUS       DAJTRC010000115        11110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.115, whole genome shotgun sequence.
ACCESSION   DAJTRC010000115 DAJTRC010000000
VERSION     DAJTRC010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 11110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.115"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO357_003381"
     CDS             complement(<1..459)
                     /gene="intS"
                     /locus_tag="OO357_003381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage integrase IntS"
                     /protein_id="HCR5888213.1"
                     /translation="MLTVKQIEAAKPKEKPYRLLDGNGLYLYVPVSGKKVWQLRYKID
                     GKEKILTVGKYPLMTLQEARDKAWTARKDISAGIDPVKAKKSSSNNNSFSAIYKEWYE
                     HKKQVWSVGYATELAKMFDDDILPIIGGLEIQDIEPMQLLEVIRRFEDRGA"
     gene            complement(621..695)
                     /locus_tag="OO357_003382"
     tRNA            complement(621..695)
                     /locus_tag="OO357_003382"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(660..662),aa:Arg,seq:cct)
     gene            complement(769..1701)
                     /locus_tag="OO357_003383"
                     /pseudo
     CDS             complement(769..1701)
                     /locus_tag="OO357_003383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311257.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
     gene            1995..2750
                     /gene="mlaA"
                     /locus_tag="OO357_003384"
     CDS             1995..2750
                     /gene="mlaA"
                     /locus_tag="OO357_003384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipid-binding lipoprotein MlaA"
                     /protein_id="HCR5888214.1"
                     /translation="MKLRLSALALGTTLLVGCASSGTDQQGRSDPLEGFNRTMYNFNF
                     NVLDPYIVRPVAVAWRDYVPQPARNGLSNFTGNLEEPAVMVNYFLQGDPYQGMVHFTR
                     FFLNTILGMGGFIDVAGMANPKLQRTEPHRFGSTLGHYGVGYGPYVQLPFYGSFTLRD
                     DGGDMADALYPVLSWLTWPMSVGKWTLEGIETRAQLLDSDGLLRQSSDPYIMVREAYF
                     QRHDFIANGGELKPQENPNAQAIQDDLKDIDSE"
     gene            complement(2932..3990)
                     /locus_tag="OO357_003385"
     CDS             complement(2932..3990)
                     /locus_tag="OO357_003385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888215.1"
                     /translation="MLPSISINNTSAAYPESINENNNDEINGLVQEFKNLFNGKEGIS
                     TCIKHLLELIKNAIRVNDDPYRSNINNSSVTYIDIGSNDTDHITIGIDNQEPIELPAN
                     YKDKELVRTIINDNIVEKTHDINNKEMIFSALKEIYDGDPGFIFDKISHKLRHTVTEF
                     DESGKSEPTDLFTWYGKDKKGDSLAIVIKNKNGNDYLSLGYYDQDDYHIQRGIRINGD
                     SLTQYCSENARSASAWFESSKAIMAESFATGSDHQVVNELNGERLREPNEVFKRLGRA
                     IRYNFQVDDAKFRRDNVKEIISTLFANKVDVDHPENKYKDFKNLEDKVEKRLQNRQTK
                     YQNEINQLSALGVNFDDI"
     gene            complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO357_003386"
     CDS             complement(4356..5696)
                     /gene="fadL"
                     /locus_tag="OO357_003386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708225.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid transporter FadL"
                     /protein_id="HCR5888216.1"
                     /translation="MSQKTLFTKSALAVAVALISTQAWSAGFQLNEFSSSGLGRAYSG
                     EGAIADDAGNVSRNPALITMFDRPTFSAGAVYIDPDVNISGTSPSGRSLKADNIAPTA
                     WVPNMHFVAPINDQFGWGASITSNYGLATEFNDTYAGGSVGGTTDLETMNLNLSGAYR
                     LNNAWSFGLGFNAIYARAKIERFAGDLGQLVAGQIMQSPAGQTPQGQALAATANGIDS
                     NTKIAHLNGNQWGFGWNAGILYELDKNNRYALTYRSEVKIDFKGNYSSDLNRAFNNYG
                     LPIPTATGGATQSGYLTLNLPEMWEVSGYNRVDPQWAIHYSLAYTSWSQFQQLKATST
                     SGDTLFQKHEGFKDAYRIALGTTYYYDDNWTFRTGIAFDDSPVPAQNRSISIPDQDRF
                     WLSAGTTYAFNKDASVDVGVSYMHGQSVKINEGPYQFESEGKAWLFGTNFNYAF"
     gene            6068..6352
                     /locus_tag="OO357_003387"
     CDS             6068..6352
                     /locus_tag="OO357_003387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005129756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcZ/YiiS family protein"
                     /protein_id="HCR5888217.1"
                     /translation="MSKCSADETPVCCCMDVGTIMDNSDCTASYSRVFANRAEAEQTL
                     AALTEKSRSVKSEPCKISPTFTEESDGVRLDIDFTFACEAEMLIFQLGLR"
     gene            6532..7842
                     /gene="fadI"
                     /locus_tag="OO357_003388"
     CDS             6532..7842
                     /gene="fadI"
                     /locus_tag="OO357_003388"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase FadI"
                     /protein_id="HCR5888218.1"
                     /translation="MGQVLPLVTRQGDRIAIVSGLRTPFARQATAFHGIPTVDLGKMV
                     VGELLARSEIPAEVIEQLVFGQVVQMPEAPNIAREIVLGTGMNVHTDAYSVSRACATS
                     FQAVANVAESLMAGTIRAGIAGGADSSSVLPIGVSKKLAYVLVDVNKARTMSQRLKLF
                     SRLRLRDLMPVPPAVAEYSTGLRMGDTAEQMAKTYGITREQQDALAHRSHQRAAQAWS
                     DGKLKEEVMTAFIPPYKQPLVEDNNIRGNSSLADYAKLRPAFDRKHGTVTAANSTPLT
                     DGAAAVILMTESRAKELGLVPLGYLRSYAFTAIDVWQDMLLGPAWSTPLALERAGLTM
                     SELTLIDMHEAFAAQTLANIQLLGSERFAREVLGRAHATGEVDDSKFNVLGGSIAYGH
                     PFAATGARMITQTLHELRRRGGGFGLVTACAAGGLGAAMVLEAE"
     gene            7842..9986
                     /gene="fadJ"
                     /locus_tag="OO357_003389"
     CDS             7842..9986
                     /gene="fadJ"
                     /locus_tag="OO357_003389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid oxidation complex subunit alpha FadJ"
                     /protein_id="HCR5888219.1"
                     /translation="MEMASAFTLNVRLDNIAIITIDVPDEKMNTLKAEFASQVRAIIK
                     QLRENKELRGVVFISAKPDNFIAGADINMIGNCKTAQEAEALARQGQQLMAEIHALPI
                     PVIAAIHGACLGGGLELALACHGRVCTDDPKTVLGLPEVQLGLLPGSGGTQRLPRLIG
                     VSTALEMILTGKQLRAKQALKLGLVDDVVPHSILLEAAVELAKKDRPSSRPLPVRERI
                     LAGPLGRALLFKMVGKKTEHKTQGNYPATERILEVVETGLAQGTSSGYDAEARAFGEL
                     AMTPQSQALRSIFFASTDVKKDPGSDAPPAPLNSVGILGGGLMGGGIAYVTACKAGLP
                     VRIKDINPQGINHALKYSWDQLEGKVRRRHLKASERDKQLALISGTTDYRGFAHRDLI
                     IEAVFENLELKQQMVAEVEQNCAAHTIFASNTSSLPIGDIAAHAARPEQVIGLHFFSP
                     VEKMPLVEIIPHAGTSAQTIATTVKLAKKQGKTPIVVRDKAGFYVNRILAPYINEAIR
                     MLTEGERVEHIDAALVKFGFPVGPIQLLDEVGIDTGTKIIPVLEAAYGERFSAPANVV
                     SSILNDDRKGRKNGRGFYLYGQKGRKSKKQVDPAIYPLIGAQGQGRLSAPQVAERCVM
                     LMLNEAVRCVDEQVIRSVRDGDIGAVFGIGFPPFLGGPFRYIDSLGAGEVVAIMQRLA
                     TQYGSRFTPCERLVEMGARGESFWKTTATDLQ"
     gene            10189..10674
                     /gene="sixA"
                     /locus_tag="OO357_003390"
     CDS             10189..10674
                     /gene="sixA"
                     /locus_tag="OO357_003390"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohistidine phosphatase SixA"
                     /protein_id="HCR5888220.1"
                     /translation="MQVFIMRHGDAALDSASDSVRPLTTNGCDESRLMANWLKGQKVE
                     IERVLVSPFLRAEQTLEEVGDCLNLPSSAEVLPELTPCGDVGLVSAYLQALTNEGVAS
                     VLVISHLPLVGYLVAELCPGETPPMFTTSAIASVTLDESGNGTFNWQMSPCNLKMAKA
                     I"
BASE COUNT         2778 a         2542 c         2747 g         3043 t
ORIGIN      
        1 tgcaccgcga tcttcaaatc tgcggattac ttccagcagt tgcatcggct caatatcctg
       61 aatctcaagg ccgccgatga tgggtaaaat gtcgtcatca aacatttttg caagttcagt
      121 cgcataccct actgaccaga cttgcttctt gtgttcgtac cattccttgt aaatcgcact
      181 aaatgaattg ttgttagacg aagacttttt cgcctttacc ggatcgatgc cagccgagat
      241 gtctttcctc gcagtccatg ctttatccct tgcctcctgc aaagtcataa gcggatattt
      301 tccgacggtc aggattttct ccttaccgtc aatcttgtag cgaagctgcc ataccttttt
      361 cccggataca gggacataaa ggtacaggcc attaccatcg agaaggcggt atggtttttc
      421 tttcggcttt gctgcttcaa tctgcttaac ggtgagcatg ggtaaaaatc cggtgggtaa
      481 aattatttta tccacttttt acccgtcatg gagtgcggct gtcaacgatc tgacgcgaac
      541 catgacgaac tgtgaatata cggaaggctt gatattcagg ggattttgcg gactggtacg
      601 gatgggagcg aactgataaa tggtgtcccc tgcaggaatc gaacctgcaa ttagccctta
      661 ggaggggctc gttatatcca tttaactaag aggacaatgc ggcgagtata cccgctaatg
      721 gactgcgggg taagtacgct gccgctcgat tgcttaaacc ctcgccattt atgctgggtt
      781 tttatcattt ttcttaatgt tttccgcacg ttctgctttt tggcgtgctt ctgctttacg
      841 cttgttgctc atgtcgttac gaatctgtgc atgactcatt aacgcgaaga taaaggtgcc
      901 gccgcagatg ttccccgcta aagtaggtag tgcgaagggc cagatgaaat cgctccagtg
      961 cagcgtaccg ttaaacacca gatagaggat ttcaacagaa ccgaccacga tatgggtggt
     1021 gtcacccagg gcaataagcc aggtcatcaa tataatcacc acaatctttg ccgcacctgc
     1081 tgcaggaaac atccatacca tagtggcgat cagccagccg gaaatgatcg cgttggcaaa
     1141 catctcgctg ggggtgttct tcatcacatc catgccgatt ttgacaaatg catcgcgagt
     1201 ttcttcattg aagataggca tatattcaaa tgcccacgca gcaataccag tcccgagaat
     1261 attacccagc agcacgactc cccataaccg cataagtaag ccaacgttgc tcattgtcgg
     1321 tttttgcatg acgggtagta ccgcagtcac ggtgttttcg gtaaataatt gctggcgggc
     1381 cattatgacg ataataaaac caaaggtata accgagattc tccagcaaga agctgcccgg
     1441 cacaccttcc agttcggcat gaaatatccc ttttgccagt aacgaagcgc ccatcgacag
     1501 acccgccgca atggctgacc acagtagcgc cattgcgtcg cgttctagct ctttttcacc
     1561 atcctggcgg atatgctcat gaattgccat cgctcgggag gggagtcggt cttcatctat
     1621 ttctattttt ttgccgcgct ctttttcttc gctctaaact tcaatttcgt cgctgtgttg
     1681 atcaattttg tcgttgtcca tggtctcttc tcttgaatta gcacgtatag ctaaagcgta
     1741 gcggcttttt tgctcgcaac tggcgggagt tactctgaaa atgtagaaaa ggctgcgttt
     1801 gcctttttct gtttctatag aatcaagtag cctacagggc ggcgattacc aggctatgat
     1861 caaatcagca aatcagggcg tctggacatc agttgacgtg ctgttacaat cgcccacacc
     1921 taaacaggcg gatacggtat cgttccgtca tggatggcaa actgcataag ccataaaaaa
     1981 acagggagac atttatgaag cttcgcctgt cggcgcttgc tctgggaact acgcttctgg
     2041 tggggtgtgc gagttccggt acagatcagc aagggcgttc tgacccgtta gaagggttca
     2101 accgcaccat gtacaacttc aacttcaacg tattagaccc gtatattgtt cgaccggtcg
     2161 ctgtcgcctg gcgtgattat gttccgcaac cggcgcgtaa tggtttgagc aactttactg
     2221 gcaaccttga agaacctgcg gtgatggtta actacttctt gcagggcgac ccttatcagg
     2281 ggatggtcca ctttacccgc tttttcctga acaccatttt ggggatgggc ggctttattg
     2341 atgttgcagg gatggcgaac ccgaaactgc aacggactga acctcaccgc ttcggtagta
     2401 cgcttggtca ttatggcgtg ggttatgggc cttacgttca gttaccgttc tacggtagct
     2461 tcacgctgcg tgatgacggt ggtgatatgg cggatgctct ttacccggtt ctttcctggc
     2521 tgacctggcc gatgtctgtg ggtaaatgga cgcttgaagg gatcgaaacc cgcgctcagc
     2581 tgctggattc cgatggtctg ctgcgtcagt cgtccgatcc ttatattatg gtgcgcgaag
     2641 cgtacttcca gcgtcatgat ttcatcgcta atggcggcga actcaaaccg caggaaaatc
     2701 cgaacgcgca agcgattcag gatgatttaa aagatatcga ttctgaataa gaaacaaata
     2761 aaaaaggtga gtcgcaaaac tcaccttttt tgttgttatc cctcaattac cacaagtaaa
     2821 aggagataaa ccgacaatga cccggacgta atgatgtaaa aatactgatg tttgtcactg
     2881 actatttccg atattcaggc gttgaaaaat caaaaaactg gaaaaataat tttaaatatc
     2941 atcaaaatta acacctagtg cagatagttg attaatttca ttttgatatt tcgtctggcg
     3001 attttgaagt cttttttcaa ctttatcttc gagattctta aagtctttgt atttattttc
     3061 aggatggtca acgtcaactt tgttagcgaa taaagtagaa attatttctt ttacattatc
     3121 gcgtctaaat tttgcatcgt ccacttgaaa attatatctt attgctcgac ctaaacgttt
     3181 aaaaacttcg ttaggttctc tcagtctttc cccgttgagc tcgtttacaa cctgatgatc
     3241 ggaaccagtt gcaaatgatt ctgccatgat agctttactg ctttcaaacc acgctgaagc
     3301 actcctggcg ttttcactac aatattgggt gagactatca ccattaatac gaattcctct
     3361 ttgaatgtgg tagtcgtcct gatcgtagta accgagagat aagtaatcat ttccgttttt
     3421 atttttaatt acaatagcga gagagtcgcc ttttttatct ttaccgtacc aggtaaataa
     3481 gtcagttggt tcgcttttcc cgctctcatc aaattccgtt accgtatgtc tgagtttgtg
     3541 tgatatctta tcgaagataa aaccaggatc tccatcatat atttctttta atgcgctgaa
     3601 gatcatttcc ttgttattga tatcatgagt cttctcaact atgttgtcat taatgatagt
     3661 acggacgagt tctttgtctt tatagttcgc aggtaattct attggttctt ggttgtcgat
     3721 accaatagta atatggtctg tatcattgga gccaatatca atataagtaa ctgaggaatt
     3781 attaatatta gatctataag gatcgtcgtt tactcgtatg gcgtttttta taagctcaag
     3841 tagatgttta atacaggtgc ttattccttc cttaccatta aaaaggtttt tgaactcttg
     3901 tactaatcca ttaatttcat cattgttatt ttcattgatg gattctgggt aagctgcgct
     3961 ggtattgttg attgaaatag atggtagcat gattttattc catgttgatt gatttgttac
     4021 aaatatatat tacctaatga aatagactat caaaaaacgc tcatttattt agaacctatc
     4081 tttaatttga gtgtataagc gcacaatagc caggggggtt gagtatcctg gaaatgcaga
     4141 aaagtaaact ctttgagaag gacgagcaga acgcttgaaa atatgcattt cccttcaggt
     4201 ttgtctgtgg ataccactta ctgatttgaa agggcaagct gccatcaggc tgtttgcaaa
     4261 actcaggagg caagcaatga ggaaactact ttcggtgaag tgggggagtt aaagtcacct
     4321 gcatagcagg tgactttatc caggcgaacg cgttatcaga acgcgtagtt aaagttagta
     4381 ccgaacagcc aggctttacc ttcagactcg aactggtatg ggccttcgtt aattttcacg
     4441 ctctgaccgt gcatataaga aacaccaacg tcgactgaag catctttatt aaatgcgtaa
     4501 gtcgtacctg cactcagcca gaaacggtcc tggtccggaa tggagataga acgattctga
     4561 gccggaactg ggctgtcatc aaaggcgata ccggtacgga aggtccagtt atcatcgtag
     4621 taataagtgg tacccaacgc gatgcggtaa gcatctttaa agccttcatg tttctggaac
     4681 agcgtgtcgc cactggttga ggtcgctttc agctgctgga actgactcca gctggtgtaa
     4741 gccaggctat agtgaatcgc ccactgtgga tcaacacggt tataacctga cacttcccac
     4801 atttcaggca ggttcagcgt cagataaccc gattgcgttg cgccacctgt cgcggtagga
     4861 attggcaaac cgtagttatt aaacgcacga ttaagatcgc tgctgtagtt acctttgaag
     4921 tcaattttca cttcagaacg gtaggtcagt gcatagcggt tatttttatc cagttcatac
     4981 aggattccgg cgttccagcc aaagccccac tggttaccgt tcagatgagc gattttggta
     5041 ttactgtcga taccgttggc ggtagctgcc aatgcttgcc cttgaggagt ttgcccggca
     5101 ggagattgca taatctgacc agcaaccaac tgccccagat cgcctgcgaa acgttcaatt
     5161 ttcgcgcgag cgtagatggc gttgaaacca agaccaaagc tccatgcatt atttaagcga
     5221 tacgcaccgc ttaagttcag gttcatggtt tcaaggtcag ttgtaccccc gacagagccg
     5281 cctgcataag tatcattaaa ctctgttgcc agaccatagt tagaggtaat agaagcgccc
     5341 caaccaaatt ggtcgttaat cggtgcaaca aagtgcatgt tcggaaccca tgccgtaggc
     5401 gcgatgttat cggctttcag gctacgacca gacggagacg ttccgctgat attcacatcc
     5461 gggtcaatat aaaccgcacc cgcagaaaat gtcgggcggt caaacatggt aatcaatgcg
     5521 gggttacggc taacgttacc tgcatcatcg gcaattgcgc cttcccctga ataagcccgg
     5581 cccaggccag aggaagaaaa ttcgtttaac tgaaagcctg ccgaccaggc ctgggtggag
     5641 ataagtgcca ctgcgactgc gagagcagac tttgtaaaca gggttttctg gctcatgacc
     5701 ataacctcaa tgatttattt ttatacaaac tatgttacgt gctgtaacag gagcgcgaag
     5761 tgtagggtct gaggtacatc ttagaaatca gaccagtggc gagagtatag gtcggaccag
     5821 ctagaatgtt gcaagtatgt ttccatcttt tttcaaatat gatctaagaa acgcgatcca
     5881 gctggcaaaa tttggaggtt gcgcactcgc ggattcgggt gaattttgtt ttagatcatt
     5941 tttaagtgtg atttcggtca cttatccgat tttgcagaat taacaactgg agcagcactt
     6001 tccggaagcg taaaatatcc acaacgttta tctggcacct tgggtgcaca tacagaggaa
     6061 atcaacgatg agtaaatgta gtgctgatga aaccccggtt tgctgctgta tggatgttgg
     6121 caccattatg gacaactccg actgcaccgc gtcttacagc cgcgtgtttg caaaccgcgc
     6181 agaagcagaa caaacgctgg cggcgctgac tgaaaaatcc cgtagcgtga aatccgaacc
     6241 gtgcaaaatc tccccgactt tcactgaaga gtctgacggt gtgcgcctgg atatcgattt
     6301 cacttttgcc tgcgaagctg aaatgctgat cttccagctg ggtctgcgtt aattcctctc
     6361 gggtacgtac gcccctgtca gttgctggca ggggcgtttt cttttctttc ttctgtcttc
     6421 actgtgtcat agctcccact ttcctttttc tccgcttggc taaatgtaaa aaaatggtta
     6481 agactatgat caggtcagac cactttattt atttttttac aggggagtgt tatgggtcag
     6541 gttttaccac tggttacccg ccagggcgat cgtatcgcca ttgttagcgg tttacgtacg
     6601 ccttttgccc gccaggcgac ggcttttcat ggcattccca cggttgattt agggaagatg
     6661 gtggtaggcg aactgctggc acgcagcgag atccccgctg aagtgattga acagctggtc
     6721 tttggtcagg tcgtacaaat gcctgaagcc cccaacattg cgcgtgaaat tgttctcggt
     6781 accggaatga atgtgcatac cgatgcttac agcgtcagcc gcgcttgcgc taccagtttc
     6841 caagcggttg caaacgtcgc agaaagcctg atggcgggaa ctatccgagc ggggattgcc
     6901 ggtggggcag attcctcttc cgtattgcca attggcgtca gtaaaaaact ggcgtacgtg
     6961 ctggttgatg tcaacaaagc tcgaaccatg agccagcgcc tgaaactctt ctctcgcctg
     7021 cgtttgcgcg acttaatgcc cgtaccgcct gcggtagcag aatattctac tggcttgcgg
     7081 atgggcgaca ccgccgaaca aatggcgaaa acctacggca tcacccgaga acagcaagat
     7141 gcattagcgc accgttcgca tcagcgtgcc gctcaggcat ggtcagacgg aaaactcaaa
     7201 gaagaggtga tgactgcctt tatccctcct tataaacaac cgcttgtcga agacaacaat
     7261 attcgcggta attcctcgct tgccgattac gcaaagctgc gcccggcgtt tgatcgcaaa
     7321 cacggaacgg taacggcggc aaacagtacg ccgctgaccg atggcgcggc agcggtgatc
     7381 ctgatgactg aatcccgggc gaaagaatta gggctggtgc cgctggggta tctgcgcagc
     7441 tacgcattta ctgcgattga tgtctggcag gacatgttgc tcggtccagc ctggtcaaca
     7501 ccgctggcgc tggaacgtgc cggtttgacg atgagcgaac tgacattgat cgatatgcac
     7561 gaagcctttg cagctcagac actggcgaat attcagttgc tgggtagtga acgttttgct
     7621 cgtgaagtac tggggcgtgc acatgccact ggcgaagtgg acgatagcaa atttaacgtg
     7681 cttggcggtt cgattgctta cggacatccc ttcgcggcga ccggcgcgcg gatgattacc
     7741 cagacattgc atgaacttcg ccgtcgcggc ggtggatttg gtttagttac cgcctgtgct
     7801 gccggtgggc ttggcgcggc aatggttctg gaggcggaat aatggaaatg gcatcagcgt
     7861 ttactcttaa tgttcgtctg gacaacattg ctatcatcac catcgacgta ccggatgaga
     7921 aaatgaatac cctgaaggcg gaatttgcct cgcaggtgcg cgccattatt aagcaactcc
     7981 gtgaaaacaa agagttgcgc ggcgtggtgt ttatctccgc gaaaccggac aattttatcg
     8041 ccggagcaga catcaacatg attggcaact gcaaaacggc gcaagaagcg gaagcactgg
     8101 cgcggcaggg ccaacagttg atggcggaga ttcatgcttt gcccattccg gttatcgcgg
     8161 ctattcatgg cgcttgcctg ggcggtgggc tggagctggc gctggcgtgc cacggtcgcg
     8221 tttgtactga cgatcctaaa acggtgctcg gtttgcctga agtacaactt ggattgttac
     8281 ccggttcagg cggcacccag cgtttaccgc gtctgatagg cgtcagtaca gcattagaga
     8341 tgattctcac cggaaaacaa cttcgggcga aacaggcatt aaagctgggg ctggtggatg
     8401 acgttgttcc gcactccatt ttgctggaag ccgctgttga gctggcgaag aaggatcgcc
     8461 catcttcccg ccctctacct gtacgcgagc gtattctggc ggggccgtta ggtcgtgcgc
     8521 tgctgttcaa aatggtcggc aagaaaacag aacacaaaac tcaaggcaat tatccggcga
     8581 cagaacgcat cctggaggtt gttgaaacgg gattagcgca gggcaccagc agcggctatg
     8641 acgctgaagc tcgcgccttt ggcgaactgg cgatgacgcc gcaatcgcaa gctttgcgta
     8701 gtatcttttt tgccagtacg gacgtgaaga aagatcccgg tagtgatgca ccgcctgcgc
     8761 cattaaacag cgtgggaatt ttaggtggtg gcttgatggg cggcggtatt gcttatgtca
     8821 ctgcttgtaa agcggggctt ccggtcagaa ttaaagatat caacccgcag ggcataaatc
     8881 atgcgctgaa gtacagttgg gatcagctgg agggcaaagt tcgccgtcgt catctcaaag
     8941 ccagcgaacg tgacaaacag ctggcattaa tctccggaac gacggactat cgcggctttg
     9001 cccatcgcga tctgattatt gaagcggtgt ttgaaaatct cgaattaaaa caacagatgg
     9061 tggcggaagt ggagcaaaat tgcgccgctc ataccatttt tgcttcgaac acgtcatctt
     9121 taccgattgg tgatatcgcc gctcacgccg cgcgacctga gcaagttatt ggcctgcatt
     9181 tcttcagtcc ggtggaaaaa atgccgctgg tagagattat tcctcatgcg ggtacatcgg
     9241 cgcaaaccat cgccactacg gtaaaactgg cgaaaaaaca gggcaaaacg cccattgtcg
     9301 tgcgtgacaa agccgggttt tacgtcaatc gcatcttagc gccttacatt aatgaagcta
     9361 tccgcatgct gaccgaaggt gaacgggtag agcacattga tgccgcgcta gtgaaatttg
     9421 gttttccggt aggcccaatc caacttttgg atgaggtagg aatcgacacc gggactaaaa
     9481 ttattcctgt actggaagcc gcttatggag aacgttttag cgcgcctgca aatgttgttt
     9541 cttcaatttt gaacgacgat cgcaaaggca gaaaaaatgg tcggggtttc tatctttatg
     9601 gtcagaaagg gcgtaaaagc aaaaaacagg tcgatcctgc catttacccg ctcattggcg
     9661 cacaagggca ggggcgactc tccgcaccgc aggttgctga acggtgtgtg atgttgatgc
     9721 tgaatgaagc agtacgttgt gttgatgagc aggttatccg tagcgtgcgt gacggggata
     9781 ttggtgcggt atttggcatt ggttttccgc catttctcgg tggaccgttc cgctatatcg
     9841 attctctcgg cgcgggcgaa gtggttgcaa taatgcaacg actggccacg cagtatggtt
     9901 cccgttttac cccttgcgag cgtttggtcg agatgggcgc gcgtggggaa agtttttgga
     9961 aaacaactgc aactgacctg caataagaag gtcaaagcta tatgaatccg cgctgaatgg
    10021 cggagtgttg gtcaaaatgt aaacgcatat tgactatact tacgccattg aggtaaaaaa
    10081 cagcgtttca ttcggtgaat ggataaggca caatgccggc caccgttttc tttctctggt
    10141 ttcagatgaa agaaaacggg cgaatctggt taacaaaagc ggtgcaatat gcaagttttt
    10201 atcatgcgtc acggcgacgc agccctcgat tccgccagtg attcggttcg tcctctgacc
    10261 actaatggtt gtgacgaatc tcgcctgatg gcgaactggc tgaaaggtca aaaagtggaa
    10321 atcgaacgcg ttctggtgag cccgttcctg cgagccgagc aaacactgga agaagtaggg
    10381 gattgtctga acctgccttc cagtgcagaa gttctgccgg agttaacgcc ctgcggcgat
    10441 gttggtctgg tcagtgccta tttgcaggcg ttgaccaatg aaggtgttgc ttcggtactg
    10501 gtgatctctc acttaccgtt agtcgggtat ctggttgccg agttatgtcc gggcgaaacg
    10561 ccgcctatgt ttaccacctc ggccattgcc agcgtaacgc ttgatgaaag cggaaacggt
    10621 acattcaact ggcagatgag cccgtgtaat ctgaagatgg caaaagctat ctgatgtgtt
    10681 ccaaattatg cccaatgagg tagattcatt gggcataatt gctcattagg cagcggatat
    10741 ctgtcaacct cactactatc gaagactctc cttatctata aataaaacac tttctacatt
    10801 cattcggaaa ttattcttct acgcgtatct tgattttgcc caaagttaca tgttgaaatg
    10861 aataatatct gtttgacact cattgtcatt tattaataca gataatagag aaaatagagt
    10921 tgaataatca ggcaggatgt aattgaaact tgattaaatt ctggctatag tgtttatata
    10981 ttatattgtc aaaagtggat aaatgccaat cgcattatat gttcattgtt tgttttttaa
    11041 gctgttgata ttcattattg atatactttc tattttttct aaatacttat tccgtatttt
    11101 ttaaggatgt
//
LOCUS       DAJTRC010000116        10936 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.116, whole genome shotgun sequence.
ACCESSION   DAJTRC010000116 DAJTRC010000000
VERSION     DAJTRC010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.116"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(3..1217)
                     /gene="rspA"
                     /locus_tag="OO357_003391"
     CDS             complement(3..1217)
                     /gene="rspA"
                     /locus_tag="OO357_003391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="starvation-sensing protein RspA"
                     /protein_id="HCR5888221.1"
                     /translation="MKIVKAEVFVTCPGRNFVTLKITTEDGITGLGDATLNGRELSVA
                     SYLQDHLCPRLIGRDAHRIEDIWQFFYKGAYWRRGPVTMSAISAVDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGHSIDEALDDYARHQELGFKAIRVQCGIPGMKTTYG
                     MSKGKGLAYEPATKGQWPEEQLWSTEKYLDFMPKLFDAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRMFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIDYIRTTLTHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCMAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPHNWTFDNGYMHPGDKPGLGIEFDEKLAAKYPYEPAYLP
                     VARLEDGTLWNR"
     gene            complement(1423..1749)
                     /locus_tag="OO357_003392"
     CDS             complement(1423..1749)
                     /locus_tag="OO357_003392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707471.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HCR5888222.1"
                     /translation="MIKTTLLFFATALCEIIGCFLPWLWLKRNASIWLLLPAGISLAL
                     FVWLLTLHPAASGRVYAAYGGVYVCTALMWLRVVDGVKLSLYDWTGALIALCGMLIIV
                     AGWGRT"
     gene            1884..2225
                     /locus_tag="OO357_003393"
     CDS             1884..2225
                     /locus_tag="OO357_003393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1283 family protein"
                     /protein_id="HCR5888223.1"
                     /translation="MKITLSKRIGLLAFLLPCALALSTTVHAETNKLVIESGDSAQSR
                     QHAAMEKEQWNDTRNLRQKVNKRTEKEWDKADAAFDNRDKCEQSANINAYWEPNTLRC
                     LDRRTGRVITP"
     gene            2291..2820
                     /gene="speG"
                     /locus_tag="OO357_003394"
                     /pseudo
     CDS             2291..2820
                     /gene="speG"
                     /locus_tag="OO357_003394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226795.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="spermidine N1-acetyltransferase"
     gene            complement(2823..3533)
                     /locus_tag="OO357_003395"
     CDS             complement(2823..3533)
                     /locus_tag="OO357_003395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnfC family lipoprotein"
                     /protein_id="HCR5888224.1"
                     /translation="MKYKLLPCLLAIFLTGCDRTEVTLSFTPEMASFSNEFDFDPLRG
                     PVKDFTQTLMDEQGEVTKRVSGTLSEEGCFDSLELLDLENNTVVALVLDANYYRDAET
                     LEKRVRLQGKCQLAELPSAGVSWETDDNGFVIKASSKQMQMEYRYDDQGYPLGKTTKS
                     NDKTLSVSATPSTDPIKKLDYTAVTLLNNQRVGNVKQSCEYDSHANPVDCQLIIVDEG
                     VKPAVERVYTIKNTIDYY"
     gene            3641..3946
                     /locus_tag="OO357_003396"
     CDS             3641..3946
                     /locus_tag="OO357_003396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707474.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1161 domain-containing protein"
                     /protein_id="HCR5888225.1"
                     /translation="MKLSTCCAALLLALASPAVLAAPGSCERIQSDISQRIINNGVPE
                     SSFTLSIVPNDQVDQPDSQVVGHCANDTHKILYNRTTSGNVSAPAQSTQDGAPAEPQ"
     gene            4145..4465
                     /locus_tag="OO357_003397"
     CDS             4145..4465
                     /locus_tag="OO357_003397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005091980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dimethylsulfoxide reductase"
                     /protein_id="HCR5888226.1"
                     /translation="MSKNDRMVGISRRTLVKSTATGSLALAAGGFSLPFTLRSAAAAV
                     QQASEKVIWGSCSVNCGSRCALRLHVKDNEVTWVETDNTGSDEYGNHQGTRLFARSLH
                     PPAY"
     gene            5025..5603
                     /locus_tag="OO357_003398"
                     /pseudo
     CDS             5025..5603
                     /locus_tag="OO357_003398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000606644.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
     gene            complement(6289..7278)
                     /locus_tag="OO357_003399"
     CDS             complement(6289..7278)
                     /locus_tag="OO357_003399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707477.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888227.1"
                     /translation="MTTIKDAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYVLQLAK
                     TVKAMLNGVLKDAQENIPFPVEVLPTRNSLPTPIIAHTLADRSVVVPVRGGKRPEVVT
                     VPSGQEIVVEPIEQAILISEQTKLWDAKSSTGFTQGTLQQDAMNICENVVRTINARMV
                     DVLESSKLLKTVELPVLTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESQLKA
                     LSRLAAKQGFSGEDAIVDMLGTDIAYYNGEDKGVFMLAKRFTALSFGCFRHDGENITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLKFTKTEA"
     gene            complement(7363..7854)
                     /locus_tag="OO357_003400"
     CDS             complement(7363..7854)
                     /locus_tag="OO357_003400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888228.1"
                     /translation="MMFNNNNWKLSVTDINLYENTVSLDGQPYPLSLAIKTLIPGYLS
                     GLPSTSREAMELLEALAEAGVTIGNFFSNDLMTAYGRRQMNKRAEAERIAKEQRLQAE
                     RMREENMTDAEWQKELQRREQVKAERRTYGESLRSATHSAGRSRAAIVADLESGGNWM
                     DSL"
     gene            complement(8175..8675)
                     /locus_tag="OO357_003401"
     CDS             complement(8175..8675)
                     /locus_tag="OO357_003401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707479.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888229.1"
                     /translation="MTSDNKQEYTIHIFGQDITSQDGLFRLDEIRRVGIQIGKVEDNR
                     STEVARFMRTKAGRSLTNPENGFVKKINMGHLGTSWLADPVAAIEFGRWLDLGYGLAV
                     TRAFVSMTSSATVQSAVASAEDIKPETKEFIQRHGTGLYIAGREVSQAEYSDYQAVTA
                     GRCEWR"
     gene            complement(8665..9180)
                     /locus_tag="OO357_003402"
     CDS             complement(8665..9180)
                     /locus_tag="OO357_003402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707480.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888230.1"
                     /translation="MNTELPVTNRQFLIILLTNAESSVDCCRYNLDLLKLSRWYAQAE
                     DWDYEKIREMYAEPIQSYLQTHTEEELKRGLQDIFYFNNPYTCEGDSEEMDWIASRSD
                     ADLKHISNAMMQVESKWSRRLQVLTEDYENGTFDKLVPPDAERLQAKADQEQRFKSFA
                     EALAGGASYDF"
     gene            complement(9611..9979)
                     /locus_tag="OO357_003403"
     CDS             complement(9611..9979)
                     /locus_tag="OO357_003403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888231.1"
                     /translation="MARPIATHDNTFTKAYLQQHCGDLLSFDGQGDLSGWLDDVLTGA
                     GRLNESMASNTKPVSPYLILTQLLTHDTLTVSAVQESLSRKRVALGEPMVSTRYARYV
                     YATVVSASKSVQYHASKAGS"
     gene            complement(10391..>10936)
                     /locus_tag="OO357_003404"
     CDS             complement(10391..>10936)
                     /locus_tag="OO357_003404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015675229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5888232.1"
                     /translation="YQFTRHTAHTTSEARRWASLVSVVTGARSAEVCHLTKRDIVTLD
                     NGLVCIDINEDGDGKSVKNKHSVRLVPLTDGAYGFDLTSFLSWVDMQPDEGPLFGMTP
                     SAYSSWFNSRVLTEALGDSQDVSLHSLRHWLATRMKERGVNLVDAQGILGHSSQSITY
                     DLYGKGHAVGRLADVLKTALL"
BASE COUNT         2809 a         2781 c         2704 g         2642 t
ORIGIN      
        1 ccttacctgt tccacagcgt gccatcttcc agacgtgcga ctggtagata agcaggttca
       61 tagggatatt tcgccgccag cttttcatcg aattcgatac caagacccgg tttgtctccc
      121 ggatgcatat agccgttatc gaaagtccag ttgtgcggga agacttcgag catttgttcg
      181 gaataaccca tgtattcctg gacaccgaaa ttggggaccc acagatcaaa gtgcagcgcc
      241 gcagccatgc agactggtga caaatcggaa ggaccgtgtg agccagtacg tacctgatac
      301 agcgaagcaa aatcggcaat ccggcgcata ccggtaattc cgcctgcatg ggtcagcgtg
      361 gtgcggatat aatcgatgag ttgctcttca atcagttgtt tgcagtccca gatgctgttg
      421 aagacttcac ccactgcgat gggtgtgacg gtatgttggc gaatgagacg gaagcattct
      481 tggttttccg ctggcgtcgg gtcttccatc cagaacatgc gataatcttc aatgctttta
      541 ccaaagcgcg ccgcttcaat aggcgttaag cgatggtgca tgtcatgcag caaatgttca
      601 ttaaaaccaa acttgttacg taccgcgtca aacaatttcg gcatgaaatc gaggtatttc
      661 tccgtcgacc acagctgctc ttccggccac tgtcctttgg ttgcgggttc ataagccaga
      721 cctttacctt tcgacatgcc gtaggtggtt ttcataccag ggattccgca ctgcacgcgg
      781 atggctttga atcccagctc ctgatgacgg gcataatcat ccagagcttc atcaatactg
      841 tgaccggtgg tatggcaata aaccatcacc ccttcacgag acgcgccgcc gagtaactgg
      901 taaagcggca tgttggcagc tttggcttta atatcccaca gcgccatatc aaccgctgaa
      961 atggccgaca tcgtaaccgg accgcgacgc cagtaagcac ctttatagaa aaactgccag
     1021 atatcttcga tacggtgcgc atcgcgacca ataagccgcg gacaaagatg atcctgcaaa
     1081 taagaggcca cggaaagctc acgtccattg agggtggcat ccccaaggcc cgtaataccg
     1141 tcctcagtgg tgatttttaa tgtgacgaaa ttacgccccg gacaggtaac aaaaacttca
     1201 gcctttacga tcttcatgtt cgattccttg catcgcttgt cgtgatgcat gaaatctacg
     1261 caactgagct actgccatac aagtatgaag atcgaaaaaa gccggagtga tcacaaaaaa
     1321 aggcgtatat ttgcgctgtg aatggttgac aaaagatgaa atagaatacc ttttgtcagc
     1381 tgacacttcc tcgtatctta ttgataaaat ggatttatgt tcctacgtgc gcccccagcc
     1441 cgcaacaatg atcaacattc cgcaaagcgc aatcaacgca cccgtccagt cataaagact
     1501 tagtttcacg ccatccacaa cgcgcagcca catcaatgcc gtgcagacat aaacgccacc
     1561 ataagccgcg taaacacgcc cactcgccgc aggatgcaac gttaacaacc agacaaacag
     1621 cgccagtgaa atccccgccg gaagcaacag ccagatactg gcgtttcgtt ttaaccacaa
     1681 ccagggcaga aagcatccaa taatttcaca cagcgcagta gcaaaaaata gtaacgttgt
     1741 tttaatcatc tttgtcactt attgacatca tgtatagtta tagggcgaca taatatcatc
     1801 aatataaaca tcctcctggt acgttttgcg ttcgcagtgg atggtgtaga atcaccttta
     1861 atcattcata cagggaatga attatgaaaa tcactctcag caaacgaatc ggcctgctcg
     1921 cttttctgct gccttgcgca ctggcattga gcacaactgt tcatgccgaa actaacaaac
     1981 tggtgattga gtctggcgac agtgcacaaa gccgccagca cgccgctatg gaaaaagagc
     2041 aatggaatga cacgcgcaat ctgcgccaga aagtgaataa acgcactgaa aaagagtggg
     2101 ataaagccga cgccgctttt gataaccgcg ataaatgtga gcaaagcgcc aacatcaatg
     2161 cctactggga gcccaatact ttgcgctgcc tggaccgtcg aactggccgc gttattaccc
     2221 cctaacctgt tattgattta aggaatgtaa ggacacgtta tgccaagcgc ccacagttaa
     2281 gctacgcccg ctggagcgtg aagatttacg ctatgtacat caactcgaca ataacgccag
     2341 tgtgatgcgt tactggtttg aggaacccta cgaagccttt gttgaactct ctgatctgta
     2401 tgataagcgc atattcacga tcagagcgaa cggcgctttg tggtggaatg tgacggcgaa
     2461 aaagccggtc tggtggagct ggtggaaatt aaccatgttc atcgccgggc agaatttcag
     2521 ataattatct ccccggagta tcaggggaaa ggtctggcaa cccgtgccgc caaattagca
     2581 atggactatg gcttcaccgt tctcaatctc tataagctgt atctgatcgt cgataaagag
     2641 aatgaaaaag cgattcacat ttaccgcaag cttggcttta cggttgaagg tgaattgatg
     2701 catgagtttt ttattaatgg tcaatatcgt aatgccattc gcatgtgtat attccagcat
     2761 cagtatctgg cagagcacaa aacaccgggt cagactctcc tgaagccgac cgcacaatag
     2821 cattaataat aatcgatcgt atttttgatg gtgtaaaccc gttcgacggc gggttttact
     2881 ccttcatcaa caatgatcag ctgacagtcc actggattgg cgtgactgtc atattcacag
     2941 ctctgtttta cattaccaac tcgttgatta tttagtaaag taaccgctgt gtaatctaat
     3001 tttttgatcg gatccgttga tggcgtggcg ctgacagata atgttttgtc gttacttttc
     3061 gtggttttac ccagcggata cccctgatca tcatagcgat attccatctg catttgtttg
     3121 ctgctggctt taatcacgaa gccattatca tcggtttccc aactcacccc ggcagaaggt
     3181 aattctgcta gctggcattt tccctgtaaa cgtactctct tctccagcgt ctcggcatca
     3241 cggtaataat tggcgtccag taccagagcg accacggtat tattttccag atccagtaat
     3301 tcgagtgaat caaaacagcc ttcttccgac aaagtcccag aaacacgttt cgtcacttca
     3361 ccttgctcat ccattaatgt ctgagtgaaa tcttttaccg gaccacgcag cggatcaaaa
     3421 tcgaattcat tagagaaact ggccatctca ggggtaaatg aaagtgttac ctctgtgcgg
     3481 tcacatcctg tgaggaatat cgcgagtaag catggtagta atttgtattt cacaacagtc
     3541 accagagagt agagatgatt ctcaatcata gtagcaaata cagtacttta cacgttaaat
     3601 gctatgctta aagaagttat cttcgcgtaa ggagcttatg atgaaactct caacctgctg
     3661 tgccgcgctt ctgctcgccc tcgcctcacc cgcggtactc gccgcgccgg gatcttgtga
     3721 gcgcatacag agcgatatat cacagcgcat tatcaataat ggcgtacccg aaagcagctt
     3781 cacgttaagt attgtaccca atgaccaggt tgatcagcct gattcccagg tcgtcgggca
     3841 ttgcgctaat gatacgcata aaattctcta caaccgcacc accagcggta acgtctctgc
     3901 tcccgcgcag tctacccagg atggcgcgcc tgccgaaccg cagtaacaca ttatcgactg
     3961 aacgccggat atgacacatc cggcgatttg aacatacaac ataatctcac cttattactc
     4021 atacccttct attgatgtgg attaataatt cttaacccaa aatgggtaga ctccctctat
     4081 tgttagcgcg ctaaatattc aatgtataaa catttatata acaataaaga acagggagtg
     4141 agttatgtcc aaaaatgacc gaatggtggg catcagccgc agaacactcg ttaaatcaac
     4201 cgcgacaggt tctctggcgc tggctgcagg cggtttttct ttaccgttca ccctgcgcag
     4261 tgcagcggca gcggtacaac aggccagcga aaaagtgatc tggggttcct gttccgtcaa
     4321 ctgtggtagc cgctgtgcac ttcgtctaca tgttaaagat aatgaagtga cctgggtgga
     4381 aactgacaat accggcagcg atgagtacgg caaccatcag ggtacgcgcc tgtttgcgcg
     4441 gtcgctccat ccgccggcgt attaatcatc ccgatcgctt gaatgaccca atgaaacgcg
     4501 tgggtaaacg cggtgaaggc aaattcgaac ggattagctg ggatgaagcc ctggatacta
     4561 tcgccagtag cctgaaaaaa accgtcgaac aatatggcaa tgaagctgta tatattcagt
     4621 actcttcggg gattgttggc ggcaatatga cccgctcttc gccatcagcc tcggcggtca
     4681 aacgcctgat gaactgctac ggcggttcac tcaatcagta tggctcctac agcaccgcgc
     4741 aaatttcctg tgccatgccc tacacctacg gcagtaatga tggcaacagc accacggata
     4801 ttgaaaacag caagctggtc gtgatgtttg gtaacaaccc ggcagaaacc cgcatgagcg
     4861 gtggtggcat cacttatctt cttgaaaaag cgcgcgagaa atcgaacgcc aaaatgattg
     4921 ttatcgatcc gcgatatacc gatacggctg ccggtcgtga agaggaatgg ctccctattc
     4981 gcccgggcac cgatgccgcg ctggttgcgg gtattgcctg ggtattgatt aacgaaaatc
     5041 tcgttgatca accttttctc gataaatact gcgtcggcta tgacgaaaaa accttacccg
     5101 cagatgcacc caaaaatggt cactataaag cctatattct tggtgaaggt gacgataaaa
     5161 cagcaaaaac gccgcagtgg gcttcgcaaa ttaccggtat cccggtagac cgtatcatca
     5221 aactggcgcg tgaaattggc acaacaaaac ccgcttatat ctgctagggc tgggggccgc
     5281 aacgccaggc aaacggcgag ctgactgcac gcgctattgc tatgttacct attttgacgg
     5341 gcaatgtcgg catcagcggc ggaaatagtg gcgcgcgtga atcgacctat accattacga
     5401 tagaacgcct accggtgctg gataatccgg tcaaaacgtc aatctcctgc ttcagctgga
     5461 cggatgctat cgatcatggc ccgcaaatga cggcactccg cgacggtgtc cggggcaaag
     5521 ataaactgga tgtgcccatt aagttcatct ggaactacaa ggacttgaag cggaccctta
     5581 gtaggcctga ggtcctgcat tgagtcaaga ccttgtgtca ttctttgtgc cagacttgtg
     5641 tcattagacg ggatgtctag acggctggtc aggaccctcc ggtggcctcg gtctggactt
     5701 gaggcgggct atgggggaag tctgctggct taatgtatta gatggtcatt cacatttttc
     5761 tgtcagaaac catcggtccc actgtgagta caaacctaag cccccagaag cccacccaca
     5821 gccttagaca gagtgtcgaa tacattcaca ccgagtcgac cggagaggac catatagagg
     5881 cccacagaga ggaacacgag tttatagacc agagtcagga gggaccttag gtcttcggaa
     5941 gtcatggaca taaagccccc ccctatcttc tattgcacta ccgcactaga caactagtac
     6001 aaaacaaagg cccagaccat tggtccagac cgtagttagt taatcacagt gacaagccac
     6061 cacctgaagt cctgctgata ttcagtccag acttacctct gctcatggtc ttacctccct
     6121 cggtctcctg accttgggta tcgactacag cttgctgagc gtcatctgga ggagctacag
     6181 gaggagctac aggaggcggg accgaagccc caccttgagt cccaccacca cctgtcaggc
     6241 ccttggcgat ggacctgaag cctttactaa tgctcttacc cattggtctt acgcttcggt
     6301 cttggtgaac ttcaggcgtt tgactacagg caggacggca gtagctgagc cagtgcccat
     6361 cttgatggtc gtagcagctt cagcgacaac gaacacttta ccgaggattt ccaagtcgtg
     6421 ggactgagag tcgccatcac gtgaaaggac gaccgtaata ttctcaccgt cgtgacggaa
     6481 gcagccgaag ctcagggccg tgaagcgttt agccagcatg aatacaccct tgtcttcacc
     6541 gttgtaatag gcgatgtctg tccccagcat atcgacgata gcgtcttcac cggagaagcc
     6601 ttgcttagca gccagacggg acagtgcttt cagttggctc tcatgagcga taataccgta
     6661 gtcagacacc tcagaaccga aagaagactc tgtgttctca tacagagcgt ccatgatagc
     6721 gtctgcctta gcggtcaggc taccagtcag gacaggaagc tctacggtct tcagcagctt
     6781 agaggattcc agaacgtcga ccatgcgagc gttgatggta cggaccacat tctcacagat
     6841 gttcatagcg tcctgctgaa gtgtgccctg agtgaatccg gtggatgact tggcgtccca
     6901 gagcttggtc tgctcagaga tgagaatagc ctgctcgatt ggttcgacca cgatttcttg
     6961 tccagacgga acggtcacga cttcaggtcg cttgccacca cggacaggaa cgaccacaga
     7021 gcggtcagcc agagtgtgag caatgattgg ggtcggtaag ctgttacggg tcggcaggac
     7081 ttctacaggg aacgggatgt tctcttgagc gtctttaagt acaccgttca gcatagcctt
     7141 aaccgtctta gctaactgga ggacatagac ctgcgggtca gcgttagtcg gcagaccttc
     7201 attgacctga atgttgtcgg tatcggtgat gcgtactggc tcaatgccaa actggaaagc
     7261 gtctttaatc gtagtcataa taatcaatct cctaaattag tttaaatact gtgagtcctc
     7321 aaaactgagg agtccctaac atcgggtcag ggactgtgtg ttttacaagc tgtccatcca
     7381 gttaccgcct gactcaaggt cagcgacaat agcggcacgg gagcgaccag cggaatgggt
     7441 agcactgcgg aggctctcac cataggtccg gcgttcagct ttgacctgtt cgcggcgctg
     7501 tagctctttc tgccactctg catcggtcat gttctcttca cgcatacgct cagcctgaag
     7561 tcgttgctct ttagcgatac gctcagcttc agctcgtttg ttcatctggc ggcgaccata
     7621 ggcggtcatc aagtcattac tgaagaagtt gccaatggtc acgccagctt cagccagagc
     7681 ttccagtaac tccattgcct cgcggctggt agacggcagg cctgacaggt agcccggaat
     7741 gagggtcttg atagcgagcg acagcggata gggctgaccg tccagactca cagtgttttc
     7801 ataaaggttg atgtcggtga ctgacagctt ccagttgttg ttgttgaaca tcataggtaa
     7861 ttctcctctt gagagttgag actatcggta tcgcagacgc agtagcgcct acgggtctcc
     7921 ccgcatcggt catctcaaaa ggtttaaaat taggtttggt atggtcagga ctgcttagca
     7981 aaggctcggc gtaactgatt gtctaaatca cggtccacca gcatagctat cagctcgtct
     8041 cgactgtagt aagggcgctc agggtcttcg actccaagtc tcagcagaga cagcagatga
     8101 cgtgtgggca ggccacggta acgaagggtc gctttgccga taggtgtacg agggtcaatc
     8161 atagccatga ttacttacct ccactcacag cgaccagcgg tcacagcttg atagtctgaa
     8221 tactcagcct gtgagacttc acgtccggcg atgtagaggc ctgtgccatg acgctggatg
     8281 aattccttgg tctccggctt gatgtcctca gcagacgcta cagcagactg cacggtggcg
     8341 ctggaggtca tagacacgaa ggctcgggtt actgccagtc cgtacccaag gtccaaccac
     8401 cgaccgaact ctatagcggc tactgggtca gccagccacg aggtccctaa gtgacccatg
     8461 ttaatctttt taacaaaccc gttttctggg ttggttagac ttctaccagc cttggtcctc
     8521 atgaagcgag caacctcggt actacggtta tcttctacct taccaatctg aatgcctacc
     8581 cggcgtatct catccagtcg aaacaatccg tcctgactgg tgatgtcctg accgaagata
     8641 tgaatggtgt attcctgctt attgtcagaa gtcataagag gcacctcccg ccagagcttc
     8701 ggcaaaggac ttgaagcgct gttcttggtc agctttcgcc tgaaggcgtt cggcatcagg
     8761 tgggaccagc ttatcaaagg tgccgttttc gtaatcttca gttaggacct gaaggcggcg
     8821 ggaccatttg ctctccacct gcatcatcgc gttagagatg tgcttcaagt cggcatcaga
     8881 gcgtgacgct atccaatcca tctcttctga gtcaccctca caggtgtaag gattgttgaa
     8941 atagaagatg tcctgcaggc cacgcttcag ctcttcctcg gtgtgtgtct ggaggtatga
     9001 ctggataggc tccgcgtaca tctcccggat tttctcatag tcccagtctt ccgcctgggc
     9061 ataccatcgg gataacttca gcaggtctaa gttatagcga cagcaatcga cactggactc
     9121 tgcattggtc agcaggatga ttaagaactg gcggttagtt actggtaatt cggtattcat
     9181 acaaagatgc tcctttggta aagcactttc tgtctccaca tggagtccgc accattcaga
     9241 cgcatcctaa acggacccat cagaaagtgc ttaacgaaag tgaatggtta aatgttgacg
     9301 gtctggagtg accatgttct ccatgtacgt ataaaggtgc gaccgcatgt caggacgagc
     9361 tgtaaggact ccagatggtg agcggtgata gacttaagcc aatgtagtga gtagacgagc
     9421 tactaaccga gacgactaaa gaacgctaac gagctggagg tcgatacagg aggtctgtca
     9481 gaggcggtca cgggccttga ttacttagga gagatactta atgtcggttg accagatgct
     9541 cgcatgttct gacgcatcag actcagaggc ggaggctggt ctccctatag tgatagttta
     9601 gggtccgtaa tcatgaacca gctttactgg cgtggtactg gacggattta ctggcgctga
     9661 cgacggtagc atagacgtag cgagcgtagc gagttgaaac cataggttca cccaaggcaa
     9721 cacgcttacg gctcagggac tcctgcacag cgcttacagt taatgtgtcg tgcgttagta
     9781 gttgggtcag aatcaagtat gggctgacag gcttggtatt gcttgccatt gattcgttca
     9841 ggcgaccagc accagtaagg acatcatcca gccaaccact caggtcgccc tgaccatcga
     9901 atgacagcag gtccccgcag tgctgctgca agtaggcttt ggtgaacgtg ttgtcgtgag
     9961 ttgcgattgg tcgtgccata gagtatctcc tgtagttgaa ttagttggtt cccctatagt
    10021 gataatcaag tccatcactg gtgatgcata cagtagcgcg acaggatgtg actcacaggt
    10081 tgaaccgaat gtcaaggact aaagacgcct caggaattaa ggaagtatat ataaagagag
    10141 accagaggcg actcacagtt aaacaggaat gtcaagagga gaccatagga atttggaggg
    10201 actggagttt aggacgcagt ttaaataaga ccacagacag acttagagtc ggacagccga
    10261 aggcggacca gtgggcctat agtcaggagg gattatgatt attatcaccc tccctaactt
    10321 cagtcagact tcagtgaccc aaggtctcaa ccatcaagcc gtatatggtc tccaccttgg
    10381 gtctccctac ttaaagtaaa gctgtcttaa gcacatcagc cagcctaccc acagcgtgtc
    10441 ctttcccgta caggtcatac gtaattgact gactgctgtg acctaagatg ccttgagcgt
    10501 ccaccagatt gactccacgc tctttcattc gggtcgccag ccaatgtctc agactgtgga
    10561 gagagacatc ctgagagtct cctagggcct cagtcaggac ccttgagtta aaccagctag
    10621 agtaggcgct cggagtcatc ccgaagagag gaccctcatc aggctgcatg tctacccacg
    10681 acaggaagga cgtcaggtca aacccataag cgccatcggt taacggcaca agtcggactg
    10741 aatgtttgtt cttcacagac ttcccgtccc catcttcatt gatgtcgata cagaccagtc
    10801 cattgtccaa ggttacaatg tcacgtttag tcaggtggca gacctcagca gaccgagcgc
    10861 cagtgaccac agacaccaga gacgcccatc tacgggcctc agaggtcgtg tgagccgtgt
    10921 gtcgtgtgaa ctgata
//
LOCUS       DAJTRC010000117        10781 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.117, whole genome shotgun sequence.
ACCESSION   DAJTRC010000117 DAJTRC010000000
VERSION     DAJTRC010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10781)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10781)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10781
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.117"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            143..973
                     /locus_tag="OO357_003405"
     CDS             143..973
                     /locus_tag="OO357_003405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2686 family protein"
                     /protein_id="HCR5888233.1"
                     /translation="MSMPLSNAANNQFNTNNHFLHHPKVDSELTRRYEYARLDTENIY
                     LLPLARGNNHNYDGKSVVEIRKLNIGKQSWPFNYVTDTCREFNGITTTGRMLYRNLKI
                     TSALDEIYGGICKKAHAATELAEGLRLNLFMKSPFDPVEDYTVHEITLGPGCNVPGYA
                     GTTIGYISTLPASQAKRWTNEQPRIDIYIDQIITVSGVANSSGFALAALLNANIEMGN
                     DPIIGIEAYPGTAEIHSKMGYNVIPGDEDAPLKRMTLQPSSLPELFELKNGEWNYIGK
                     "
     gene            complement(1114..1887)
                     /gene="uxuR"
                     /locus_tag="OO357_003406"
     CDS             complement(1114..1887)
                     /gene="uxuR"
                     /locus_tag="OO357_003406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Uxu operon transcriptional regulator"
                     /protein_id="HCR5888234.1"
                     /translation="MKSVTSAQRPYQEVGAMIHDLIIKTPYNPGERLPPEREIAEMLD
                     VTRTVVREALIMLEIKGLVEVRRGAGIYVLDSSGSHNTDSPDANVCNDAGPFELLQAR
                     QLLESNIAEFAALQATREDIVKMRQALQLEERELASSAPGSSESGDMQFHLAIAEATH
                     NSMLVELFRQSWQWRENNPMWIQLHSHLDDSLYRKEWLGDHKQILAALIKKDARAAKL
                     AMWQHLENVKQRLLEFSNVDDIYFDGYLFDSWPLDKVDA"
     gene            complement(2102..3562)
                     /locus_tag="OO357_003407"
     CDS             complement(2102..3562)
                     /locus_tag="OO357_003407"
                     /EC_number="1.1.1.57"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructuronate reductase"
                     /protein_id="HCR5888235.1"
                     /translation="MTTIVDSNLPVARPSWDHSRLESRIVHLGCGAFHRAHQALYTHH
                     LLESTDSDWGICEVNLMPGNDRMLIENLKKQQLLYTVAEKGAESTELKIIGSMKEALH
                     PEIDGCEGILNAMARPQTAIVSLTVTEKGYCADAASGQLDLNNPLIKHDLENPTAPKS
                     AIGYIVEALRLRREKGLKAFTVMSCDNVRENGHVAKVAVLGLAQARDPQLAAWIEENV
                     TFPCTMVDRIVPAATPETLQEIADQLGVYDPCAIACEPFRQWVIEDNFVNGRPDWDKV
                     GTQFVADVVPFEMMKLRMLNGSHSFLAYLGYLGGYETIADTMTNPDYRKAAFALMMQE
                     QAPTLSMPEGTDLNAYATLLIERFSNPSLRHRTWQIAMDGSQKLPQRLLDPVRLHLQN
                     GGSWRHLALGVAGWMGYTQGVDEQGNAIDVVDPMLAEFQKINAQYQGADRVKALLGLS
                     GIFADDLPQNADFVGAVTSAYQQLCERGARECVAAL"
     gene            complement(3643..4827)
                     /gene="uxuA"
                     /locus_tag="OO357_003408"
     CDS             complement(3643..4827)
                     /gene="uxuA"
                     /locus_tag="OO357_003408"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709912.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HCR5888236.1"
                     /translation="MEQTWRWYGPNDPVSLADVRQAGATGVVTALHHIPNGEVWSVEE
                     ILKRKAIVEDAGLVWSVVESVPIHEDIKTHTGNYEQWIANYQQTLRNLAQCGIRTVCY
                     NFMPVLDWTRTDLEYVLPDGSKALRFDQIEFAAFEMHILKRPGAEADYTEEEIAQAAE
                     RFATMSDEDKARLTRNIIAGLPGAEEGYTLDQFRKHLELYKDIDKAKLRENFAVFLKA
                     IIPVAEEVGVRMAVHPDDPPRPILGLPRIVSTIEDMQWMVDTVNSMANGFTMCTGSYG
                     VRADNDLVDMIKQFGPRIYFTHLRSTMREDNPKTFHEAAHLNGDVDMYKVVKAIVEEE
                     HRRKAEGKEDLIPMRPDHGHQMLDDLKKKTNPGYSAIGRLKGLAEVRGVELAIQRAFF
                     IR"
     gene            5167..6510
                     /gene="gntP"
                     /locus_tag="OO357_003409"
     CDS             5167..6510
                     /gene="gntP"
                     /locus_tag="OO357_003409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate permease GntP"
                     /protein_id="HCR5888237.1"
                     /translation="MHVLNILWVVFGIGLMLILNLKFKINSMVALLVAALSVGMLAGM
                     DLMSLLHTMKAGFGNTLGELAIIVVFGAVIGKLMVDSGAAHQIAHTLLARLGLRYVQL
                     SVIIIGLIFGLAMFYEVAFIMLAPLVIVIAAEAKIPFLKLAIPAVAAATTAHSLFPPQ
                     PGPVALVNAYGADMGMVYIYGVLVTIPSVICAGLILPKFLGNLERPTPSFLKADQPVD
                     MNNLPSFGVSILVPLIPAIIMISTTIANIWLVKDTPAWEVVNFIGSSPIAMFIAMVVA
                     FVLFGTARGHDMQWVMNAFESAVKSIAMVILIIGAGGVLKQTIIDTGIGDTIGMLMSH
                     GNISPYIMAWLITVLIRLATGQGVVSAMTAAGIISAAILDPATGQLVGVNPALLVLAT
                     AAGSNTLTHINDASFWLFKGYFDLSVKDTLKTWGLLELVNSVVGLIIVLIISMVA"
     gene            complement(6761..7663)
                     /gene="fimH"
                     /locus_tag="OO357_003410"
     CDS             complement(6761..7663)
                     /gene="fimH"
                     /locus_tag="OO357_003410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria D-mannose specific adhesin FimH"
                     /protein_id="HCR5888238.1"
                     /translation="MKRAITLFAVLLMGWSVNAWSFACKTANGTAIPIGGGSANVYVN
                     LAPVVNVGQNLVVDLSTQIFCHNDYPETITDYVTLQRGSAYGGVLSNFSGTVKYSGSS
                     YPFPTTSETPRVVYNSRTDKPWPVALYLTPVSSAGGVAIKAGSLIAVLILRQTNNYNS
                     DDFQFVWNIYANNDVVVPTGGCDVSARDVTVTLPDYPGSVPIPLTVYCAKSQNLGYYL
                     SGTTADAGNSIFTNTASFSPAQGVGVQLTRNGTIIPANNTVSLGAVGTSAVSLGLTAN
                     YARTGGQVTAGNVQSIIGVTFVYQ"
     gene            complement(7683..8186)
                     /gene="fimG"
                     /locus_tag="OO357_003411"
     CDS             complement(7683..8186)
                     /gene="fimG"
                     /locus_tag="OO357_003411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimG"
                     /protein_id="HCR5888239.1"
                     /translation="MKWCKRGYVLAAMLALASATIQAADVTITVNGKVVAKPCTVSTT
                     NATVDLGDLYSFSLMSAGAASAWHDVALELTNCPVGTSRVTASFSGAADSTGYYKNQG
                     TAQNIQLELQDDSGNTLNTGATKTVQVDDSSQSAHFPLQVRALTVNGGATQGTIQAVI
                     SITYTYS"
     gene            complement(8199..8729)
                     /gene="fimF"
                     /locus_tag="OO357_003412"
     CDS             complement(8199..8729)
                     /gene="fimF"
                     /locus_tag="OO357_003412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria minor subunit FimF"
                     /protein_id="HCR5888240.1"
                     /translation="MRNKPFYLLCAFLWLAVSRVLAADSTITIRGYVRDNGCSVAAES
                     TNFTVDLMENAAKQFNNIGATTPVVPFRILLSPCGNAVSAVKVGFTGVADSHNANLLA
                     LENTVSAAAGLGIQLLNEQQNQIPLNAPSSAISWTTLTPGKPNTLNFYARLMATQVPV
                     TAGHINATATFTLEYQ"
     gene            complement(8739..>10781)
                     /gene="fimD"
                     /locus_tag="OO357_003413"
     CDS             complement(8739..>10781)
                     /gene="fimD"
                     /locus_tag="OO357_003413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial usher FimD"
                     /protein_id="HCR5888241.1"
                     /translation="RIGGNSHYAYLNLQSGLNIGAWRLRDNTTWSYNSSDSSSGSKNK
                     WQHIITWIERDIIPLRSRLTLGDGYTQGDIFDGINFRGAQLASDDNMLPDSQRGFAPV
                     IHGIARGTAQVTIKQNGYGIYNSTVPPGPFTINDIYAAGNSGDLQVTIKEADGSTQIF
                     TVPYSSVPLLQREGHTRYSITAGEYRSGNAQQEKPRFFQSTLLHGLPAGWTIYGGTQL
                     ADRYRAFNFGIGKNMEALGALSVDMTQANSTLPDDSQHDGQSVRFLYNKSLNESGTNI
                     QLVGYRYSTSGYFNFADTTYSRMNGYNIETQDGVIQVKPKFTDYYNLAYNKRGKLQLT
                     VTQQLGRTSTLYLSGSHQTYWGTSNVDEQFQAGLNTAFEDINWTLSYSLTKNAWQKGR
                     DQMLALNVNIPFSHWLRSDSKSQWRHASASYSMSHDLNGRMTNLAGVYGTLLEDNNLS
                     YSVQTGYAGGGDGNSGSTGYATLNYRGGYGNANIGYSHSDDIKQLYYGVSGGVLAHAN
                     GVTLGQPLNDTVVLVKAPGAKDAKVENQTGVRTDWRGYAVLPYATEYRENRVALDTNT
                     LADNVDLDNAVANVVPTRGAIVRAEFKARVGIKLLMTLTHNNKPLPFGAMVTSESSQS
                     SGIVADNGQVYLSGMPLAGKVQVKWGEEENAHCVANYQLPPESQQQLLTQLSAECR"
BASE COUNT         2584 a         2790 c         2649 g         2758 t
ORIGIN      
        1 ttcctaccct ttaatatcac tgtgtagagt tgatatcact acaacagaaa catgcatgtc
       61 atttgtttac ttctcttttt actacagaaa aatttatcta tttttctaaa catcagttaa
      121 atactcataa aaggtaacta ttatgtctat gccattaagc aatgccgcta ataaccaatt
      181 caatacaaat aatcacttcc ttcatcatcc aaaagtcgac agtgagttaa cacgtcgata
      241 tgaatatgca cgcctggata cggaaaatat ctatcttctg cctcttgctc gtggcaataa
      301 ccataattac gatggaaaaa gcgttgtcga aattagaaaa cttaatattg gcaaacagtc
      361 ctggccattt aattatgtga cagatacatg cagagaattc aatggtatta caacaacagg
      421 acgtatgctc tatcgaaact tgaaaatcac ctcagcttta gacgaaatat atggtggtat
      481 atgtaaaaag gcgcatgctg ctacagagtt agcagaaggt ttacggctaa atttatttat
      541 gaagagccct ttcgatcctg ttgaggacta tacagttcat gaaataacat taggccctgg
      601 ttgcaatgtt cctggttatg ccggaaccac gataggatat atttcaaccc ttccagcttc
      661 tcaggcaaaa agatggacca atgagcagcc aagaattgat atctatattg atcagataat
      721 aactgtctca ggggtagcaa attcttccgg attcgccctt gccgcattac ttaatgctaa
      781 tattgagatg ggaaatgacc caatcattgg tatagaagcc tatcctggta ccgcggagat
      841 ccattcaaaa atgggatata acgttatccc cggtgatgaa gatgcgccac tcaaacgcat
      901 gactctgcaa ccgtcatctc ttccagaact gtttgaatta aaaaatggtg aatggaatta
      961 tattggtaaa tagccactga gaagaatgag tactaatatt caggttatac agatatttaa
     1021 acaaaaccct gcaatttaca aagtaatact cattgcgact tcttaattta agaaagcgca
     1081 aggaacgttt acccttgcgc ttattataat aagtcaggcg tcgactttat ccagcggcca
     1141 tgaatcaaag agatagccgt caaaataaat atcgtcaacg ttcgagaatt ccagcagacg
     1201 ttgcttaacg ttttccagat gctgccacat tgccagcttc gccgctcggg catctttttt
     1261 gattaacgcg gcgaggatct gtttgtgatc gcccaaccac tctttgcgat acaggctgtc
     1321 atccagatgg ctgtgcaact ggatccacat tggattgttt tcccgccact gccaggactg
     1381 acggaataac tccaccagca tgctgttatg cgttgcttca gcaatggcga gatggaactg
     1441 catgtcaccg ctttcgctgc tacccggcgc gctggaagcc agttcacgct cttccagttg
     1501 caatgcctga cgcattttga cgatatcttc gcgggtagcc tgcagtgcgg caaactcggc
     1561 gatgttactt tccagtaact gacgcgcctg taacagctca aaaggacccg catcattgca
     1621 gacgttggca tccggactgt ctgtgttgtg gctgcctgag ctatcaagca catagatacc
     1681 ggcaccccgg cgcacttcca ccagcccttt gatctccagc atgatcagcg cttcacgtac
     1741 caccgtccgc gtgacatcaa gcatttctgc aatttcacgc tccggcggca gccgttcgcc
     1801 aggattgtac ggcgtcttta tgatcagatc gtggatcatc gccccgactt cctggtaagg
     1861 tctttgcgca gaggtgacag atttcatggt gttgtgtccg ttaggtgcgg ttaatcaaat
     1921 ttttgccgga tgcggcgtga acgccttatc cggcctacaa cgggcaattt tacggccccc
     1981 gttaatctca aactatacca ttagcactgg aagttggcac gtacaggtgg gcaaaaaaac
     2041 gaaaactggt caaccaaata tcagtttgat tgaccagtaa gtctgtaggg taatcagtta
     2101 gttacagcgc agccacacac tcgcgcgcac cgcgttcgca cagctgctga tatgccgacg
     2161 tcaccgcgcc aacaaagtcg gcattctgcg gcagatcatc ggcaaaaata ccgctcaggc
     2221 ccagcagcgc tttcacgcgg tctgcgccct gatactgcgc gttaatcttc tggaactcgg
     2281 ccagcatcgg gtcgaccacg tcaatggcat taccctgctc atccacgccc tgggtgtaac
     2341 ccatccagcc agccacgccc agcgccaggt gacgccagct gccgccgttt tgcaggtgca
     2401 gacgcaccgg gtccagtaga cgctgcggta acttctggct gccgtccatc gcaatctgcc
     2461 aggtacggtg acgcagagac gggttgctga aacgctcgat cagcagcgtc gcataggcgt
     2521 tcaggtccgt accttccggc atcgacagcg ttggcgcttg ttcctgcatc atcagggcaa
     2581 aggccgcttt gcgataatcc gggttagtca tggtgtcggc aatggtttca tagccgccga
     2641 ggtaaccgag gtacgccagg aaagagtggc tgccgttcag catacgcagc ttcatcattt
     2701 cgaacggcac aacgtctgca acgaactgtg tgcccacttt atcccagtcc gggcgaccat
     2761 taacgaagtt atcttcaatc acccactgac ggaacggttc gcaggcaatg gcgcacgggt
     2821 cgtaaacacc cagctggtca gcaatttcct gtaaggtttc tggcgtcgcc gccggaacga
     2881 tgcggtcaac catggtgcac gggaaggtga cgttctcttc aatccatgcc gctagctgcg
     2941 ggtcacgcgc ctgagccagc cccagtaccg cgaccttcgc cacatggccg ttttcacgca
     3001 cgttatcgca ggacatcacc gtaaatgctt tcagcccttt ttcacgacgc agacgcaagg
     3061 cttcgacgat gtaaccaatc gcggacttcg gcgcagtcgg gttttccaga tcgtgcttga
     3121 tcagcgggtt attgagatcc agttgaccgc ttgccgcatc agcgcagtag cctttttccg
     3181 tgaccgttag agagacaatc gccgtttgcg gacgcgccat cgcgttgaga ataccttcgc
     3241 agccgtcgat ttccggatgc agcgcttctt tcatcgaacc gataattttc agctcggtgc
     3301 tttctgcgcc tttttccgct acggtgtaca gcagttgctg ttttttcagg ttttcgatca
     3361 gcatgcggtc gttgcctggc atcaggttaa cttcgcagat gccccagtcg ctgtcggtgc
     3421 tttccagcag atgatgggta tacagcgcct ggtgcgcgcg gtgaaacgcc ccgcaaccga
     3481 gatgcacaat gcgtgattcc agacgagaat gatcccatga cgggcgggca accggcagat
     3541 tgctgtcaac aatagtagtc atcgtaaact ccagaatccg tcttcagaga gtgacggcaa
     3601 ggaagaagga accggcacgg cgcgcagcca tgccggtggg tattaacgga taaagaaagc
     3661 gcgctggatc gccagttcga caccgcgaac ttcggccagg cctttcagac gaccaattgc
     3721 ggagtaacca gggttggttt tcttcttcag gtcgtccagc atctgatgac cgtggtccgg
     3781 acgcatcggg atcaggtctt ctttgccttc cgctttacga cggtgttctt cttcaacaat
     3841 cgctttcacc actttgtaca tatcaacgtc accgttcagg tgcgccgctt cgtggaaggt
     3901 tttcgggtta tcttcacgca tggtggagcg cagatgggtg aagtaaatac gcggaccgaa
     3961 ctgcttgatc atatcaacca gatcgttgtc agcacgcacg ccgtaggaac cggtgcacat
     4021 ggtgaaaccg ttcgccatgc tgtttacggt atcaaccatc cactgcatat cttcaatggt
     4081 ggaaacaatg cgcggcaagc cgaggatcgg gcgcggcgga tcgtccgggt gaaccgccat
     4141 acgcacgcca acttcttcag caactggaat aatcgctttc aggaagacgg caaagttttc
     4201 gcgcagtttg gctttgtcga tatctttgta cagctccagg tgcttacgga actggtcgag
     4261 ggtataccct tcttccgcgc ccggcagacc ggcaatgatg ttacgcgtca gacgcgcttt
     4321 gtcttcgtcg ctcatagtgg cgaagcgttc agctgcctga gcaatttctt cttcggtgta
     4381 atccgcttcc gcgcctggac gtttcaggat atgcatttcg aatgcagcga attcgatctg
     4441 gtcgaagcgc agagctttgg agccgtctgg cagcacgtat tcgaggtcag tacgggtcca
     4501 gtcgagcacc ggcatgaagt tgtagcacac ggtgcgaatg ccacactgcg ccaggttgcg
     4561 cagagtctgc tgatagttag cgatccactg ctcatagttg ccagtgtggg ttttgatatc
     4621 ttcgtgaatt ggtacgcttt ctacgacaga ccacaccagg cctgcgtctt caacgatcgc
     4681 cttgcgtttg aggatctctt ctacggacca tacttcgccg ttcgggatat ggtgcagcgc
     4741 ggtaaccacg ccagttgcgc ccgcctgacg gacatcagct aaagaaaccg gatcgtttgg
     4801 gccgtaccag cgccaggtct gttccatgat ttttcctctt tcacatagtg ggttccacag
     4861 caaagagacg cggaaccttg gagttgttta ggtatccgga tgaacaacca gaccgcagcc
     4921 gtggcgttgc atcggatgag atggcgtata agttctacct tttggtaaga catgtcaacc
     4981 agaattctga aattggttaa ccgcgtcgca agaatttcac tctcatggtt gttgctgcaa
     5041 attgcagcaa agggtaatcg atggttattg atcagaggat gtgacattca tcgcaacaat
     5101 ggttgaccaa tttacataac atgtcgccca aaataacaac ggttcaacca agtaaggtaa
     5161 aacgttatgc atgtgcttaa cattctctgg gtggtattcg gcattggcct gatgctgata
     5221 ctgaatttga agttcaaaat caattcaatg gtggctttgt tggtggcggc gctgtccgtc
     5281 gggatgctgg caggcatgga tttgatgtcg ctgctgcaca ccatgaaagc gggcttcggc
     5341 aacacgctgg gagaactggc tatcatcgtg gtgttcggtg cggtcatcgg taaattgatg
     5401 gtcgactccg gcgcggctca ccagatagca catacgctgc tggcgcgtct cggtctgcgc
     5461 tatgtacagc tgtcggtgat tatcatcggc cttattttcg gtctggcgat gttctatgaa
     5521 gtggccttta tcatgttagc gccgctggtt attgttattg ccgccgaagc taaaattccg
     5581 ttcctgaaac tggcgatccc ggcagtagca gctgccacta ccgcacattc actgttccca
     5641 ccgcagccgg gtccggtggc gctggtgaat gcttatggcg cggatatggg gatggtttat
     5701 atctatggcg tactggtgac gatcccaagt gtaatctgcg caggtctgat cctgccgaag
     5761 ttcctcggca atcttgagcg cccaacgcca tcattcctga aagcagatca accggtagat
     5821 atgaataatc tgccctcttt cggcgtttcg attctggtgc cgctgatccc ggcgatcatt
     5881 atgatctcta ccaccatcgc caatatctgg ctggtaaaag atacccctgc ctgggaagtg
     5941 gttaacttta ttggttcctc gccgattgcg atgtttattg cgatggtggt tgcattcgta
     6001 ctctttggca ccgcgcgtgg tcatgacatg cagtgggtga tgaacgcttt tgaaagcgcg
     6061 gtgaagagta ttgcaatggt gattctgatc atcggtgcgg gtggcgtgct gaagcagacc
     6121 atcatcgaca ccggcattgg cgacaccatc ggcatgttga tgtcccacgg caatatctcg
     6181 ccctacatta tggcatggct gatcactgtg ctaattcgtc tggcgacggg tcagggtgtc
     6241 gtttcggcga tgaccgccgc cgggattatc agtgctgcaa tccttgatcc agcaaccggt
     6301 cagctggttg gcgtgaatcc ggcgctgctg gtactggcga cggctgcggg ttccaacacc
     6361 ctcacccaca ttaatgatgc atctttctgg ttgttcaaag gttactttga cctgtcagta
     6421 aaagacacgt tgaaaacctg gggtctgctg gagctggtca actccgtggt tgggctgatt
     6481 attgtgctga ttattagcat ggtagcgtaa aagaacacca gagcctgcca atggcaggct
     6541 cagactgatg actaaagtct aaaaatgtgc ccggacagtc ccctcgcccc tccggggaga
     6601 gggttagggt gaggggaaca gaccagtaaa gtgcgctttg tttatgccag atgcgacgct
     6661 gacgcgtctt atctggccta caaagggcta acgtgcaggt tttgtaggtc tgatagcgta
     6721 gcgcctcagg taccagcatt agcaatgcgc tgtaattttt ttattgataa acaaaagtca
     6781 cgccaataat cgattgcaca ttccctgcag tcacctgccc tccggtacgt gcgtaatttg
     6841 ccgttaatcc cagacttacc gccgaagttc ctactgctcc taacgatacc gtgttattcg
     6901 ctggaataat cgtaccgttg cgcgtcaact gtacgccgac gccctgcgct ggtgaaaacg
     6961 acgcggtatt ggtgaaaatc gagttgcccg catctgcggt tgtgccggag aggtaatacc
     7021 ccaggttttg gcttttcgca caataaacgg taagaggaat tggcactgaa ccagggtagt
     7081 ccggcagagt aacggtgaca tcacgagcag aaacatcaca accgccagtg ggcaccacca
     7141 catcattatt ggcgtaaata ttccacacaa actgaaaatc atcgctgtta tagttgttgg
     7201 tctgtcgcaa aataagcacg gcaattaatg aaccagcttt aatagccacc ccgcccgcac
     7261 tgctcacagg cgtcaaataa agcgccaccg gccacggctt atccgttctc gaattataaa
     7321 caacccgcgg cgtttcgctg gtggtcggaa atggatagct actgccacta tattttacgg
     7381 tcccggaaaa attagataac acgccgccgt aagccgagcc tcgttgcagt gtgacatagt
     7441 ctgtaatggt ttccggataa tcgttatggc aaaagatttg cgtcgaaaga tctacgacca
     7501 ggttttgccc cacattcacg acaggcgcaa ggtttacata aacattagcg ctgccaccgc
     7561 caatagggat agcggtacca ttggcggttt tacaggcgaa tgaccaggca tttaccgacc
     7621 agcccatcag cagtacagca aacagggtaa tagctcgttt cattacaatt atctcttcgg
     7681 gttcagctgt aggtataggt gatgctaatc actgcctgaa tggttccctg agtggctccg
     7741 ccatttaccg tcaatgctct gacctgtaac gggaagtgcg ctgattgtga ggaatcatcc
     7801 acctgaactg ttttggttgc gccagtattc aatgtgttgc cactgtcatc ctgtagctct
     7861 aactggatgt tttgcgcggt cccctggttt ttataatatc cggtgctgtc ggctgccccg
     7921 ctgaagctgg cagtgaccct cgacgttccc accggacaat tagtcaactc aagcgcaaca
     7981 tcatgccagg ccgatgccgc cccggcagac ataagactga aagaataaag atcgccgaga
     8041 tctaccgtgg cattggtggt ggaaaccgta cacggtttgg cgacgacctt accgttcacc
     8101 gtgatggtga catcggctgc ctgtatcgtc gcacttgcga gcgccaacat tgccgccaat
     8161 acatacccac gtttgcacca tttcatgagc atctccagtt actgatattc aagagtgaag
     8221 gtagccgtag cattgatatg ccccgcagtg acaggcacct gtgtcgccat tagccgggcg
     8281 taaaaattca gcgtatttgg tttacccggc gtcagggtcg tccacgaaat cgcagacgat
     8341 ggagcattaa ggggtatttg attttgctgc tcattcagaa gctgtattcc cagtcccgca
     8401 gccgctgaca ccgtattttc aagtgcaagc aggttggcat tgtggctatc tgcaacgccg
     8461 gtaaacccaa cttttacggc agaaacggca ttaccacagg gtgacagcaa aatacgaaat
     8521 ggaacgacag gagtcgtcgc gccaatgttg ttaaattgct tcgccgcgtt ttccatcaga
     8581 tcaacagtaa aattggttga ttcagcggcc acactacagc cgttatctct gacatagccg
     8641 cggatagtaa tcgtgctatc cgcagccaaa acgcgactta ccgccagcca caaaaaagcg
     8701 cacagaagat aaaaaggttt gtttctcatc acgccccctt aacgacattc agctgatagc
     8761 tgggttaata actgctgctg actctctggt ggcagttgat aattggcgac acagtgagca
     8821 ttttcctctt ctccccattt cacctgaact tttcccgcta aaggcattcc gctgaggtaa
     8881 acctgaccat tatccgcaac aatgccgcta ctctggctac tctctgatgt caccatcgcc
     8941 ccaaacggca gcggcttatt attgtgagtt agcgtcatga gcagttttat cccaacgcgc
     9001 gctttaaact ctgctcgcac gatcgcccca cgagtgggaa caacgttagc gaccgcgtta
     9061 tctaaatcga cgttatcagc caaggtattg gtatccagcg ccactctatt ttcccgatat
     9121 tcagtggcat aaggcagcac ggcataacca cgccagtcgg tacgcacccc cgtctggttt
     9181 tcgacttttg catcttttgc gccaggcgct ttaacaagca ccaccgtatc gtttaacggc
     9241 tgccccagcg ttacgccatt ggcatgagcc aataccccac cgctgactcc gtaatagagc
     9301 tgcttaatat catcgctatg gctgtaaccg atattggcat tgccgtaacc accgcgataa
     9361 ttcagcgtgg cgtagcctgt acttccgcta ttaccatcgc ctcccccggc atagccggtt
     9421 tgcacgctat aactgaggtt gttgtcttcc agcaacgtac cgtatacacc agccagattg
     9481 gtcatccgac cgttgagatc gtgtgacatg ctgtagctgg cactggcatg tcgccactga
     9541 gatttactgt cagaacgcag ccagtggctg aaaggaatat tgacgttaag cgctaacatc
     9601 tgatcccgtc ctttttgcca ggcgtttttc gtcaggctat agctgagcgt ccagttgata
     9661 tcttcgaacg cagtatttaa tccagcctgg aattgctcat cgacattact cgttccccaa
     9721 taagtttgat ggctaccact caaatacagt gttgatgtgc gcccgagttg ctgagtaacg
     9781 gtgagctgta atttcccgcg tttgttataa gcgaggttgt aatagtcggt gaatttcggc
     9841 ttaacctgaa taactccatc ctgtgtttcg atgttgtagc cattcattcg actgtatgtt
     9901 gtatcagcga aattaaaata tccgctggtc gaataacggt aacccactaa ctgaatattc
     9961 gtgcctgatt cattgagcga tttgttatag agaaaacgca ccgattgtcc gtcatgctga
    10021 ctgtcatcgg gaagtgtgga attagcctgc gtcatatcca cagacagagc gcccagtgcc
    10081 tccatgtttt tcccgatacc gaaattaaaa gcacgataac gatccgccag ttgcgttcca
    10141 ccatatattg tccagccagc cggaaggccg tggagtaatg tactctggaa aaagcggggt
    10201 ttttcctgct gcgcatttcc actacggtat tctcctgccg taatggaata acgagtatgc
    10261 ccttcacgtt gcaaaagcgg gactgacgaa tagggtacgg taaaaatctg cgtgctgccg
    10321 tcagcctctt tgatcgttac ctgcaagtca ccactattac ctgcggcata gatatcgttg
    10381 atggtaaaag gccccggtgg caccgtacta ttataaatgc catatccatt ttgtttaata
    10441 gtgacctgtg cagtaccacg agcaataccg tggatcaccg gggcaaatcc tctttggcta
    10501 tcgggtaaca tattgtcatc tgaggccaat tgtgcgccgc gaaagttaat accatcgaaa
    10561 atatcgccct gggtataacc atcacccaac gtcagccggg aacgtaaagg aattatgtct
    10621 cgctcaatcc aggtaatgat atgctgccat ttatttttgc tacctgatga actgtcgcta
    10681 ctgttataac tccaggtggt attgtcgcgt aaacgccacg caccaatatt taacccactc
    10741 tgtaggttta aatatgcata atggctgtta cccccaatcc g
//
LOCUS       DAJTRC010000118        10622 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.118, whole genome shotgun sequence.
ACCESSION   DAJTRC010000118 DAJTRC010000000
VERSION     DAJTRC010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10622)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10622)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10622
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.118"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(116..469)
                     /locus_tag="OO357_003414"
     CDS             complement(116..469)
                     /locus_tag="OO357_003414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4156 domain-containing protein"
                     /protein_id="HCR5888242.1"
                     /translation="MHVKYLAGIVGAALLMAGCSSSNELSAAGQSVRIVDEQPGAECQ
                     LIGTATGKQSNWLSGQHGEEGGSMRGAANDLRNQAAAMGGNVIYGISSPSQGMLSSFV
                     PTDSQIIGQVYKCPN"
     gene            complement(607..2253)
                     /gene="groL"
                     /locus_tag="OO357_003415"
     CDS             complement(607..2253)
                     /gene="groL"
                     /locus_tag="OO357_003415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120975.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HCR5888243.1"
                     /translation="MAAKDVKFGNDARVKMLRGVNVLADAVKVTLGPKGRNVVLDKSF
                     GAPTITKDGVSVAREIELEDKFENMGAQMVKEVASKANDAAGDGTTTATVLAQAIITE
                     GLKAVAAGMNPMDLKRGIDKAVTAAVEELKALSVPCSDSKAIAQVGTISANSDETVGK
                     LIAEAMDKVGKEGVITVEDGTGLQDELDVVEGMQFDRGYLSPYFINKPETGAVELESP
                     FILLADKKISNIREMLPVLEAVAKAGKPLLIIAEDVEGEALATLVVNTMRGIVKVAAV
                     KAPGFGDRRKAMLQDIATLTGGTVISEEIGMELEKATLEDLGQAKRVVINKDTTTIID
                     GVGEEAAIQGRVAQIRQQIEEATSDYDREKLQERVAKLAGGVAVIKVGAATEVEMKEK
                     KARVEDALHATRAAVEEGVVAGGGVALIRVASKLADLRGQNEDQNVGIKVALRAMEAP
                     LRQIVLNCGEEPSVVANTVKGGDGNYGYNAATEEYGNMIDMGILDPTKVTRSALQYAA
                     SVAGLMITTECMVTDLPKNDAADLGAAGGMGGMGGMGGMM"
     gene            complement(2297..2590)
                     /locus_tag="OO357_003416"
     CDS             complement(2297..2590)
                     /locus_tag="OO357_003416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000027827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HCR5888244.1"
                     /translation="MNIRPLHDRVIVKRKEVETKSAGGIVLTGSAAAKSTRGEVLAVG
                     NGRILENGEVKPLDVKVGDIVIFNDGYGVKSEKIDNEEVLIMSESDILAIVEA"
     gene            2866..4122
                     /gene="yjeH"
                     /locus_tag="OO357_003417"
     CDS             2866..4122
                     /gene="yjeH"
                     /locus_tag="OO357_003417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710006.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine/branched-chain amino acid
                     transporter"
                     /protein_id="HCR5888245.1"
                     /translation="MSGLKQELGLAQGIGLLSTSLLGTGVFAVPALAALVAGNNSLWA
                     WPVLIILVFPIAIVFAILGRHYPSAGGVAHFVGMAFGSRLERVTGWLFLSVIPVGLPA
                     ALQIAAGFGQAMFGWHSWQLLLAELGTLALVWYIGTLGASSSANLQTVIAGLIVALIV
                     AIWWAGDIKPASIPFPAPGNIELTGLFAALSVMFWCFVGLEAFAHLASEFKNPERDFP
                     RALMIGLLLAGLVYWGCTVVVLHFDAYGEQMAAAASLPKIVVQLFGVGALWIACVIGY
                     LACFASLNIYIQSFARLVWSQAQHNPDHYLARLSSRHIPNNALNAVLGCCVVSTLVIH
                     ALEINLDALIIYANGIFIMIYLLCMLAGCKLLQGRYRLLAVVGGLLCVLLLAMVGWKS
                     LYALIMLVGLWLFLPKRKTPENGITT"
     gene            complement(4138..4614)
                     /gene="fxsA"
                     /locus_tag="OO357_003418"
     CDS             complement(4138..4614)
                     /gene="fxsA"
                     /locus_tag="OO357_003418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein FxsA"
                     /protein_id="HCR5888246.1"
                     /translation="MRWLPFIAIFLYVYIEISIFIQVAHVLGVLLTLVLVIFTSVIGM
                     SLVRNQGFKNFVLMQQKMAAGENPAAEMIKSVSLIIAGLLLLLPGFFTDFLGLLLLLP
                     PVQKHLTVKLMPHLRFSRMPGGGFSAGTGGGNTFDGEYQRKDDDRDRLDHKDDRQD"
     gene            4951..6387
                     /gene="aspA"
                     /locus_tag="OO357_003419"
     CDS             4951..6387
                     /gene="aspA"
                     /locus_tag="OO357_003419"
                     /EC_number="4.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313147.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate ammonia-lyase"
                     /protein_id="HCR5888247.1"
                     /translation="MSNNIRIEEDLLGTREVPADAYYGVHTLRAIENFYISNNKISDI
                     PEFVRGMVMVKKAAAMANKELQTIPKSVANAIIAACDEVLNNGKCMDQFPVDVYQGGA
                     GTSVNMNTNEVLANIGLELMGHQKGEYQYLNPNDHVNKCQSTNDAYPTGFRIAVYSSL
                     IKLVDAINQLREGFERKAVEFQDILKMGRTQLQDAVPMTLGQEFRAFSILLKEEVKNI
                     QRTAELLLEVNLGATAIGTGLNTPKEYSPLAVKKLAEVTGFPCVPAEDLIEATSDCGA
                     YVMVHGALKRLAVKMSKICNDLRLLSSGPRAGLNEINLPELQAGSSIMPAKVNPVVPE
                     VVNQVCFKVIGNDTTVTMAAEAGQLQLNVMEPVIGQAMFESVHILTNACYNLLEKCIN
                     GITANKEVCEGYVYNSIGIVTYLNPFIGHHNGDIVGKICAETGKSVREVVLERGLLTE
                     AELDDIFSVQNLMHPAYKAKRYTDESEQ"
     gene            6505..7806
                     /gene="dcuA"
                     /locus_tag="OO357_003420"
     CDS             6505..7806
                     /gene="dcuA"
                     /locus_tag="OO357_003420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter DcuA"
                     /protein_id="HCR5888248.1"
                     /translation="MLVVELIIVLLAIFLGARLGGIGIGFAGGLGVLVLAAIGVKPGN
                     IPFDVISIIMAVIAAISAMQVAGGLDYLVHQTEKLLRRNPKYITILAPIVTYFLTIFA
                     GTGNISLATLPVIAEVAKEQGVKPCRPLSTAVVSAQIAITASPISAAVVYMSSVMEGH
                     GISYLHLLSVVIPSTLLAVLVMSFLVTMLFNSKLSDDPIYRKRLEEGLVELRGEKQIE
                     IKSGAKTSVWLFLLGVVGVVIYAIINSPSMGLVEKPLMNTTNAILIIMLSVATLTTVI
                     CKVDTDNILNSSTFKAGMSACICILGVAWLGDTFVSNNIDWIKDTAGEVIQGHPWLLA
                     VIFFFASALLYSQAATAKALMPMALALNVSPLTAVASFAAVSGLFILPTYPTLVAAVQ
                     MDDTGTTRIGKFVFNHPFFIPGTLGVALAVCFGFVLGSFML"
     gene            7922..8260
                     /gene="cutA"
                     /locus_tag="OO357_003421"
     CDS             7922..8260
                     /gene="cutA"
                     /locus_tag="OO357_003421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710002.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divalent cation tolerance protein CutA"
                     /protein_id="HCR5888249.1"
                     /translation="MLDEKSSNTTSVVVLCTAPDEATAQDLAAKVLAEKLAACATLIP
                     GATSLYYWEGKLEQEYEVQMILKTTVSHQQALLECLKSHHPYQTPELLVLPVTHGDTD
                     YLSWLNASLR"
     gene            8236..9933
                     /gene="dsbD"
                     /locus_tag="OO357_003422"
     CDS             8236..9933
                     /gene="dsbD"
                     /locus_tag="OO357_003422"
                     /EC_number="1.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD"
                     /protein_id="HCR5888250.1"
                     /translation="MAQRIFTLILLLCSTSVFAGLFDAPGRSQFVPADQAFAFDFQQN
                     QHDLNLTWQIKDGYYLYRKQIRITPEHAKIADEQLPQGVWHEDEFYGKSEIYRDRLTL
                     PVTINQASAGATLTVTYQGCADAGFCYPPETKTVPLSEVVANNAASQPVSVSQQEQHT
                     AQLPFSALWALLIGIGIAFTPCVLPMYPLISGIVLGGKQRLSTARALLLTFIYVQGMA
                     LTYTALGLVVAAAGLQFQAALQHPYVLIGLAIVFTLLAMSMFGLFTLQLPSSLQTRLT
                     LMSNRQQGGSPSGVFVMGAIAGLICSPCTTAPLSAILLYIAQSGNMWPGGGTLYLYAL
                     GMGLPLMLITVFGNRLLPKSGPWMEQVKTAFGFVILALPVFLLERVIGDVWGLRLWSA
                     LGVAFFGWAFITSLQAKRGWMRVVQIILLAAALVSVRPLQDWAFGATHTAQTQTHLNF
                     TQIKTVDELNQALVEAKGKPVMLDLYADWCVACKEFEKYTFSDPQVQKALADTVLLQA
                     NVTANDAQDVALLKHLNVLGLPTILFFDGQGQEHPQARVTGFMDAETFSAHLRDRQP"
     gene            9970..10545
                     /locus_tag="OO357_003423"
     CDS             9970..10545
                     /locus_tag="OO357_003423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5888251.1"
                     /translation="MQREDVLGEALKLLELQGIANTTLEMVAERVDYPLDELRRFWPD
                     KEAILYDALRYLSQQIDVWRRQLMLDETQTAEQKLLARYQALSECVKNNRYPGCLFIA
                     ACTFYPDPGHPIHQLADQQKSAAYDFTHELLTTLEVDDPAMVAKQMELVLEGCLSRML
                     VNRSQADVDTAHRLAEDILRFARCRQGGALT"
BASE COUNT         2483 a         2791 c         2634 g         2714 t
ORIGIN      
        1 gccttatccg gcctacaaat tctcaagcat tcaatcagtt gcaatgcctg ctgtaggcct
       61 gataagcgaa gcgcatcagg caataatacg tttgtcaccc agcctctgga tcacatcagt
      121 tcgggcactt ataaacctga ccgataatct ggctatccgt cgggacaaaa ctggacaaca
      181 ttccctgcga cgggctgctg atgccataaa tcacgttacc gcccattgca gccgcctggt
      241 tgcgcagatc gtttgctgcg ccgcgcatag aaccgccctc ttctccgtgt tgcccggaaa
      301 gccagttgct ttgcttacct gtcgcagtac caatcagctg gcactctgcg cctggctgct
      361 cgtccacaat gcgtacactc tgaccggcag cactcaattc gttgctggag ctacaacccg
      421 ccatcagtag cgcggcaccg acaatccctg ctaagtattt tacgtgcatg ttattcccca
      481 taatcaatga gctggacgcc actcgcgtca tccgtgtctg aatcttatac tagaaagatg
      541 accaaaagaa aaacccccag acatttctgc ccgggggttt gtttatttct gcgaagtgca
      601 gggcaattac atcatgccgc ccatgccacc catgccgccc ataccgccag cagcgcctaa
      661 gtcagctgca tcgtttttcg gcaggtcggt aaccatgcat tcggtggtga tcatcaggcc
      721 agccacagaa gctgcgtact gcagagcaga acgggttact ttggttgggt ccaggatacc
      781 catgtcgatc atgttgccgt attcttcggt tgctgcgttg taaccgtagt tgccgtcgcc
      841 gcctttaacg gtgttagcaa caacagacgg ttcttcgccg cagttcagga cgatctgacg
      901 cagcggagct tccattgcac gcagtgcaac tttgataccc acgttctggt cttcgttctg
      961 accacgcagg tcagccagtt tagacgctac gcggatcagc gcaacaccac caccagcaac
     1021 cacgccttct tctaccgcag cacgggtcgc gtgcagggca tcttcaacgc gtgctttttt
     1081 ctctttcatt tcaacttcgg tagcagcacc tactttgata actgcaacgc cgcctgccag
     1141 tttcgctacg cgctcctgca gtttttcacg gtcgtagtca gaagttgctt cttcaatctg
     1201 ctgacggatc tgagcaacac ggccctggat tgcagcttct tcacccacgc catcgatgat
     1261 agtggtggtg tctttgttga tcacaacacg tttagcctga cccaggtctt ccagggttgc
     1321 tttttccagc tccataccga tctcttcaga gattacggta ccgccagtca gggttgcgat
     1381 atcctgcagc atagctttac gacgatcgcc gaagcccgga gctttaaccg cagcaacttt
     1441 cacgatgcca cgcatggtgt taacaaccag agttgctagc gcttcgcctt caacatcttc
     1501 agcgatgatc agcagcggtt tgcctgcttt ggcaacagct tccagaaccg gcagcatttc
     1561 gcggatgttg gagattttct tgtcagccag caggatgaac gggctttcca gttctactgc
     1621 gccagtttcc ggcttgttga tgaagtaagg agacaggtag ccacggtcga actgcatacc
     1681 ttcaaccacg tccagttcgt cctgcagacc ggtaccgtct tcaacggtga taacgccttc
     1741 tttaccgact ttgtccatcg cttcagcgat cagtttacct acggtttcgt cggagttagc
     1801 ggagatggta ccaacctgag caatcgcttt agaatcagag cacggtacgg acagcgcttt
     1861 cagttcttca actgcagcgg taaccgcttt gtcgatacca cgtttcaggt ccatcgggtt
     1921 catgcccgca gcaacagctt tcagaccttc agtgatgata gcctgagcca gtacggttgc
     1981 agtggtggta ccgtcgcctg cagcgtcgtt cgctttggag gcaacttctt tcaccatctg
     2041 cgcacccata ttttcgaact tgtcttccag ttcgatttca cgagcaacgg aaacaccatc
     2101 tttggtgatg gtcggtgcac cgaaagattt atccagaact acgttacggc cttttggacc
     2161 gagggtaact ttcactgcat ctgccagtac gtttacgccg cgcagcattt tcacacgagc
     2221 gtcgttaccg aattttacgt ctttagctgc cattatcttt attccttaaa ttcgtatgtt
     2281 cagtgtcgtg cgcggattac gcttcaacaa ttgccagaat gtcgctttcg gacatgatca
     2341 acacttcttc attgtcgatc ttctcggatt tcacaccgta gccatcgttg aaaataacga
     2401 tgtcgccaac tttcacgtcc agcggcttca cttcgccatt ttcaaggata cggccattgc
     2461 cgacagccag cacttcgccg cgggtggatt tagccgctgc agagccggtc agaacgatgc
     2521 cgccagcaga tttagtttca acttctttac gcttgacgat cacgcgatca tgcaatggac
     2581 gaatattcat tgataactct cctttgagaa agtccgtatc tgttatgggt gacgccggag
     2641 cttacgtggt ttcccggctg gtgaccagag aaatggggat gaggcttcgc cccttcaagg
     2701 gggaaaaaga aaaaaaattc tgatcagcac aaaatcgggt gaaaaccctg attcacctca
     2761 catttcatcg caatttcttc atcgccgtca taagcgaatc tgattgtgct accatcgaaa
     2821 atctacgcag ttgcccaaaa tttgggcgca atcggatatc aacccatgag tggactcaaa
     2881 caagaactgg ggctggccca gggcattggc ctgctatcga cgtcattatt aggcactggc
     2941 gtgtttgccg ttcctgcgtt agctgcactg gtagcaggca ataacagcct gtgggcgtgg
     3001 cccgttttga ttatcttagt gttcccgatt gcgattgtgt ttgcgattct gggtcgccac
     3061 tatcccagcg caggcggcgt cgcgcacttc gtcggtatgg cgtttggttc gcggcttgag
     3121 cgagtcaccg gctggctgtt tttatcggtc attcccgtgg gtttgcctgc cgcactacaa
     3181 attgccgccg gattcggcca agcaatgttt ggctggcata gctggcaact gttgttggca
     3241 gaactcggta cgctggcgtt ggtgtggtat atcggtactc tcggtgccag ttccagtgct
     3301 aatctacaaa ccgttattgc cggacttatc gtcgcactga ttgtcgctat ctggtgggcg
     3361 ggcgatatca aacctgcgag tatccccttc cccgcgccag gaaatatcga acttaccggg
     3421 ttatttgctg cgttatcagt gatgttctgg tgttttgtcg gtctggaagc atttgcccat
     3481 cttgcctcgg aatttaaaaa tccagagcgt gattttcctc gtgctttgat gattggtctg
     3541 ctgctggcag gattagtcta ctggggctgt acggtagtcg tcttacactt cgacgcctat
     3601 ggtgaacaaa tggcggcggc agcatcgctt ccaaaaattg tagtacagtt gttcggtgta
     3661 ggagcgttat ggattgcctg cgtaattggc tatctggcct gctttgccag tcttaacatt
     3721 tatatacaga gcttcgcccg cctggtctgg tcgcaggcgc aacataatcc tgaccactac
     3781 ctggcacgcc tctcttctcg ccatatcccg aataatgccc tcaatgcggt gcttggctgc
     3841 tgcgtggtga gcacgttggt aattcatgcc ttagagatca atctggacgc tcttattatt
     3901 tatgccaatg gcatctttat tatgatttat ctgttatgca tgctggcagg ctgtaaatta
     3961 ttgcaaggtc gttatcgact actggcggtg gttggcgggc tgttatgcgt tctgttactg
     4021 gcaatggtcg gctggaaaag tctctatgcg ctgatcatgc tggtggggtt atggctgttt
     4081 ctgccaaaac gaaaaacgcc ggaaaatggc ataaccacat aatccggcgt ttcgacatta
     4141 atcctggcga tcgtctttat gatcaaggcg gtcgcggtca tcatcctttc gctgatactc
     4201 accatcaaaa gtattaccgc caccggtccc ggcgctaaaa ccgccgcctg gcatgcgaga
     4261 aaagcgcaaa tgtggcatca acttcactgt cagatgcttt tgcaccggcg gcaataaaag
     4321 cagaagaccg aggaagtcgg tgaaaaagcc cggtaataaa agcagcaaac cggcaataat
     4381 cagcgaaaca cttttaatca tctccgccgc agggttttca cccgccgcca ttttttgctg
     4441 catcagcacg aaattcttaa agccctggtt acgtaccagt gacataccga taactgacgt
     4501 gaatataacc agcacgaggg tcagcaatac ccccaataca tgggcaacct gaataaagat
     4561 tgaaatctca atatagacat aaaggaaaat ggcaataaaa ggtaaccagc gcaaaggttt
     4621 ctcctgtaat agcagccggt taaacccggc tacctgaatg ggttgcgaat cgccttcagc
     4681 ttatattgtg gtcattagca aaatttcaag atgtttgcgc aactattttt ggtagtaatc
     4741 ccaaagcggt gatctatttc acaaattaat aattaagggg taaaaaccga cacttaaagt
     4801 gatccagatt acggtagaaa tcctcaagca gcatatgatc tcgggtattc ggtcgatgca
     4861 ggggataatc gtcggtcgaa aaacattcga aaccacatat attctgtgtg tttaaagcaa
     4921 atcattggca gcttgaaaaa gaaggttcac atgtcaaaca acattcgtat cgaagaagat
     4981 ctgttgggta ccagggaagt tccagctgat gcctactatg gtgttcacac tctgagagcg
     5041 attgaaaact tctatatcag caacaacaaa atcagtgata ttcctgaatt tgttcgcggt
     5101 atggtaatgg ttaaaaaagc cgcagctatg gcaaacaaag agctgcaaac cattcctaaa
     5161 agtgtagcga atgccatcat tgccgcatgt gatgaagtcc tgaacaacgg aaaatgcatg
     5221 gatcagttcc cggtagacgt ctaccagggc ggcgcaggta cttccgtaaa catgaacacc
     5281 aacgaagtgc tggccaatat cggtctggaa ctgatgggtc accagaaagg tgaatatcag
     5341 tacctgaacc cgaacgacca tgttaacaaa tgtcagtcca ctaacgacgc ctacccgacc
     5401 ggtttccgta tcgcagttta ctcttccctg attaagctgg tagatgcgat taaccaactg
     5461 cgtgaaggct ttgaacgtaa agctgtcgaa ttccaggaca tcctgaaaat gggtcgtacc
     5521 cagctgcagg acgcagtacc gatgaccctc ggtcaggaat tccgcgcttt cagcatcctg
     5581 ctgaaagaag aagtgaaaaa catccaacgt accgctgaac tgctgctgga agttaacctt
     5641 ggcgcaacag caatcggtac tggtctgaac acgccgaaag agtactctcc gctggcagtg
     5701 aaaaaactgg ctgaagtcac tggcttccca tgcgtaccgg ctgaagacct gatcgaagcg
     5761 acctctgact gcggcgctta tgttatggtt cacggcgcgc tgaaacgcct ggctgtgaag
     5821 atgtccaaaa tctgtaacga cctgcgcttg ctctcttctg gtccacgtgc cggcctgaac
     5881 gagatcaacc tgccggaact gcaggcgggc tcttccatca tgccagctaa agtaaacccg
     5941 gttgttccgg aagtggttaa ccaggtatgc ttcaaagtca tcggtaacga caccactgtt
     6001 accatggcag cagaagcagg tcagctgcag ttgaacgtta tggaaccggt cattggccag
     6061 gccatgttcg aatccgttca cattctgacc aacgcttgct acaacctgct ggaaaaatgc
     6121 attaacggca tcactgctaa caaagaagtg tgcgaaggtt acgtttacaa ctctatcggt
     6181 atcgttactt acctgaaccc gttcatcggt caccacaacg gtgacatcgt gggtaaaatc
     6241 tgtgccgaaa ccggtaagag tgtacgtgaa gtcgttctgg aacgcggtct gttgactgaa
     6301 gcggaacttg acgatatttt ctccgtacag aatctgatgc acccggctta caaagcaaaa
     6361 cgctatactg atgaaagcga acagtaatcg tacagggtag tacaaataaa aaaggcacgt
     6421 cagatgacgt gccttttttc ttgtgagcag taacttaaaa ataacaatct aatatcaact
     6481 tgttaaaaaa caaggaaggc taatatgcta gttgtagagc tcatcatagt tttgctggcg
     6541 atcttcttgg gcgccagatt ggggggaata ggtattggtt ttgcaggcgg attgggggtg
     6601 ctggttcttg ccgctattgg cgttaaaccc ggtaacatcc cgttcgatgt catttccatt
     6661 atcatggcgg ttatcgccgc tatttctgcc atgcaggttg ctggcggtct ggactatctg
     6721 gttcatcaga cagaaaagct gctgcgccgt aacccgaaat acatcacgat cctcgcaccg
     6781 atcgtgacct atttcctgac tatctttgct ggtactggca acatctctct ggcgacactg
     6841 ccagttatcg ctgaagttgc gaaggaacaa ggcgttaaac cttgccgtcc gctctctact
     6901 gcagtggtat ccgcgcagat tgcgatcacc gcatcgccaa tctcagcggc agtggtttac
     6961 atgtcttccg tgatggaagg tcatggcatc agctacctcc atctgctctc cgtggtcatc
     7021 ccgtccaccc tgctggcggt tctggtgatg tccttcctgg tcactatgct gttcaactcc
     7081 aaactctctg acgatccgat ttatcgcaag cgtctggaag agggcttggt tgaactgcgc
     7141 ggtgaaaagc agattgaaat caaatccggt gcaaaaacgt ccgtctggct gttcctgctg
     7201 ggcgtagttg gcgtggttat ctatgcaatc atcaacagcc caagcatggg tctggttgaa
     7261 aaaccgctga tgaacaccac caacgcaatc ctgatcatca tgctcagcgt tgcaactctg
     7321 accaccgtta tctgtaaagt cgataccgac aacatcctca actccagcac cttcaaagca
     7381 ggtatgagcg cctgtatttg tatcctgggt gttgcgtggc tgggcgatac tttcgtttcc
     7441 aacaacatcg actggatcaa agataccgct ggtgaagtga ttcagggtca tccgtggctg
     7501 ctggccgtca tcttcttctt tgcttctgct ctgctgtact ctcaggctgc aaccgcaaaa
     7561 gcactgatgc cgatggctct ggcactgaac gtttcaccgc tgaccgctgt tgcttctttc
     7621 gctgcggtgt ctggtctgtt cattctgccg acctacccga cgctggttgc tgcggtacag
     7681 atggatgaca cgggtactac ccgtatcggt aaattcgtct tcaaccatcc gttcttcatc
     7741 ccgggtactc tgggtgttgc cctggccgtt tgcttcggct tcgtgctggg tagcttcatg
     7801 ctgtaatgat caatcgcggg gcgttcacgc cccgctttct ttcccgccga ctaacatcct
     7861 tcccccgtcc gttgtatagt gacctctctc ttgcggttcc atctgttctt gcgaggtgtt
     7921 tatgcttgat gaaaaaagtt cgaataccac gtctgtcgtg gtgctatgta ccgcaccgga
     7981 tgaagcgaca gcccaggatt tagccgccaa agtgctggcg gaaaaactgg cggcctgcgc
     8041 gaccttgatc cccggcgcta cctctctcta ttactgggaa ggtaagctgg agcaagaata
     8101 cgaagtgcag atgattttaa aaactaccgt atctcaccag caggcactgc tggaatgcct
     8161 gaagtctcat catccatatc aaaccccgga acttctggtt ttacctgtta cacacggaga
     8221 cacagattac ctctcatggc tcaacgcatc tttacgctga ttctgctact ttgcagcact
     8281 tccgtttttg ccggattatt cgacgcgccg ggacgttcac aatttgtccc cgcggatcaa
     8341 gcctttgctt ttgattttca gcaaaaccaa catgacctta atctgacctg gcagatcaaa
     8401 gacggttact acctctaccg taaacagatc cgcattacgc cggaacacgc gaaaattgcc
     8461 gacgagcagc tgccgcaagg cgtctggcat gaagatgagt tttacggcaa aagcgagatt
     8521 taccgcgatc ggctgacgct tcccgtaacc atcaaccagg cgagtgcggg agcgacgtta
     8581 actgtcacct accagggctg tgctgatgcc ggtttctgtt atccgccaga aaccaaaacc
     8641 gttccgttaa gcgaagtggt cgccaacaac gcagcgtcac agcctgtgtc tgtttcgcag
     8701 caagagcagc acaccgcgca attgcccttt tccgcgctct gggcgttgtt gatcggtatt
     8761 ggtatcgcct ttacgccatg cgtgctgcca atgtacccac tgatttctgg catcgtgctg
     8821 ggtggtaaac agcggctctc cactgccaga gcattgttgc tgacctttat ttatgtgcag
     8881 gggatggcac tgacttacac ggcgctgggt ctggtagttg ccgccgcagg gttacagttc
     8941 caggcggcgc tacagcaccc atacgtgctc attggcctcg ccatcgtctt taccttgctg
     9001 gcgatgtcaa tgtttggctt gtttactcta caactcccct cttcgctgca aacacgtctc
     9061 acgttgatga gcaatcgcca acagggcggc tcacctagcg gtgtgtttgt tatgggggcg
     9121 attgccggac tgatctgttc accatgcacc accgcaccgc ttagcgcgat tctgctgtat
     9181 atcgcccaaa gcgggaacat gtggccgggc ggcggcacgc tttatctcta tgcgttgggc
     9241 atgggcctgc cgctgatgct aattaccgtc tttggtaacc gcttgctgcc gaaaagcggc
     9301 ccgtggatgg aacaagtcaa aaccgcgttt ggttttgtga tcctcgcact gccggtcttc
     9361 ctgctggagc gagtgattgg tgatgtatgg ggattacgct tgtggtcggc gctgggtgtc
     9421 gcattctttg gctgggcctt tatcaccagc ctacaggcta aacgcggctg gatgcgcgtg
     9481 gtgcaaataa tcctgctggc agcggcattg gttagcgtgc gcccacttca ggattgggca
     9541 tttggtgcga cgcataccgc gcaaactcaa acgcatctca actttacaca aatcaaaacg
     9601 gtagatgagt taaatcaggc gctcgttgaa gccaaaggca aaccggtgat gttagatctt
     9661 tatgccgact ggtgcgtcgc ctgtaaagag tttgagaaat acaccttcag cgacccgcag
     9721 gtgcaaaaag cgttagcaga cacggtctta cttcaggcca acgtcacggc caacgacgca
     9781 caagatgtgg cgctgttaaa gcatcttaat gtccttggcc taccgacaat tctctttttt
     9841 gacggacaag gccaggagca tccacaagca cgcgtcacgg gctttatgga tgctgaaacc
     9901 ttcagcgcac atttgcgcga tcgccaaccg tgaacgacac ttgcagtggg aaagacggag
     9961 gaaaatagcg tgcaacgtga agatgtactg ggagaagccc tgaaattatt agaattacaa
    10021 gggattgcca acaccacgct ggagatggtt gctgaacgtg tggattatcc actggacgag
    10081 ctacgccgct tctggccaga caaagaggca atcctctacg atgcgctgcg ttatcttagc
    10141 caacagatag atgtctggcg tcgtcagctg atgctggacg aaacgcaaac cgccgaacaa
    10201 aagctgctgg cacgttatca ggcgttatcg gagtgcgtta aaaacaaccg ctatccgggc
    10261 tgtctattta tcgctgcctg tacgttttat cccgatcctg gccaccctat tcatcaactg
    10321 gccgatcagc aaaaaagcgc tgcctacgat ttcacccacg aactgttaac cacgctggaa
    10381 gttgacgatc cggcgatggt agcaaagcag atggaactgg tgctggaagg ctgtttaagc
    10441 cgaatgctgg tgaatcgtag ccaggcggat gtcgacactg cacatcggct ggcggaagat
    10501 atcctgcgct tcgcccgctg ccgccagggt ggtgcattga cctgacagaa acacagaaaa
    10561 gaagcgattt gccgcaatct taagcagttg aatcgctttt actgaaatta ggttgacgag
    10621 at
//
LOCUS       DAJTRC010000119        10464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.119, whole genome shotgun sequence.
ACCESSION   DAJTRC010000119 DAJTRC010000000
VERSION     DAJTRC010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.119"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            3..203
                     /gene="cspG"
                     /locus_tag="OO357_003424"
     CDS             3..203
                     /gene="cspG"
                     /locus_tag="OO357_003424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001990088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock protein CspG"
                     /protein_id="HCR5888252.1"
                     /translation="MTGLVKWFNADKGFGFITPDDGSKDVFVHFTAIQSNEFRTLNEN
                     QKVEFSIEQGQRGPAAANVVTL"
     gene            214..402
                     /locus_tag="OO357_003425"
     CDS             214..402
                     /locus_tag="OO357_003425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HCR5888253.1"
                     /translation="MTQHLHFRCPCCHGSQYRTSAFDVTERNPLGAKCIFCKSTMITF
                     DNVALQIRTDHAPLDFTK"
     gene            377..607
                     /gene="ymcE"
                     /locus_tag="OO357_003426"
     CDS             377..607
                     /gene="ymcE"
                     /locus_tag="OO357_003426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YmcE"
                     /protein_id="HCR5888254.1"
                     /translation="MRRWISQNNIRLPRGAFFISALFFFNAVCIVSDNLLIIESFGKM
                     AYNISYLTRVPGTNTLLACCCLSRPDEVNSEY"
     gene            597..770
                     /locus_tag="OO357_003427"
     CDS             597..770
                     /locus_tag="OO357_003427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module toxin, GnsA/GnsB family"
                     /protein_id="HCR5888255.1"
                     /translation="MNIEELKKQAETEIADFIAQKIAELNKNTGKEVSEIRFIAREKM
                     TGLESYDVKIKIM"
     gene            complement(819..1892)
                     /locus_tag="OO357_003428"
     CDS             complement(819..1892)
                     /locus_tag="OO357_003428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HCR5888256.1"
                     /translation="MAEKKRTRWQRRPGTTGGKLPWNDWRNATTWRKATQFLLLAMNI
                     YIAITFWYWVRYYETAGSTTFVARPGGIEGWLPIAGLMNLKYSLATGQLPSVHAAAML
                     LLVAFIVISLLLKKAFCSWLCPVGTLSELIGDLGNKLFGRQCVLPRWLDIPLRGVKYL
                     LLSLFLYIALLMPAQAIHYFMLSPYSVVMDVKMLDFFRHMGTATLISVTVLLIASLFI
                     RHAWCRYLCPYGALMGVVSLLSPFKIRRNAESCIDCGKCAKKCPSRIPVDKLIQVRTV
                     ECTGCMTCVESCPVASTLTFSLQKPAANKKAFALSGWLMTLLVLGIMFAVIGYAMYAG
                     VWQSPVPEELYRRLIPQAPMIGH"
     gene            complement(1994..4705)
                     /gene="torS"
                     /locus_tag="OO357_003429"
                     /pseudo
     CDS             complement(1994..4705)
                     /gene="torS"
                     /locus_tag="OO357_003429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415513.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system sensor histidine
                     kinase/response regulator TorS"
     gene            4788..5816
                     /gene="torT"
                     /locus_tag="OO357_003430"
     CDS             4788..5816
                     /gene="torT"
                     /locus_tag="OO357_003430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TMAO reductase system periplasmic protein TorT"
                     /protein_id="HCR5888257.1"
                     /translation="MRVLLFLLLSLFMLSAFSADNLLRWHDAQHFTVQASMPLKAKRV
                     WKLCALYPSLKDSYWLSLNYGMQEAARRYGVDLKVLEAGGYSQLATQQAQIDQCKQWG
                     AEAILLGSSTTSFPDLQKQVANLPVIELVNALDDPQVKSRVGVPWFQMGYQPGRYLVQ
                     WAHGKPLNVLLMPGPDNAGGSKEMVEGFRAAIAGSPVRIVDIALGDNDIEIQRNLLQE
                     MLERHPEIDVVAGTAIAAEAAMGEGRNLKTPLTVVSFYLSHQVYRGLKRGRVIMAASD
                     QMVWQGELAVEQAIRQLQGQSVSDNVSPPILVLTPKNADREHIRRSLSPGGFRPVYFY
                     QHTSAAKK"
     gene            complement(5789..6481)
                     /gene="torR"
                     /locus_tag="OO357_003431"
     CDS             complement(5789..6481)
                     /gene="torR"
                     /locus_tag="OO357_003431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator TorR"
                     /protein_id="HCR5888258.1"
                     /translation="MPHHIVIVEDKPVTQARLQSYFTQEGYTVSVTASGAGLREIMQN
                     QPVDLILLDINLPDENGLMLTRALRERSTVGIILVTGRSDRIDRIVGLEMGADDYVTK
                     PLELRELVVRVKNLLWRIDLARQAQPHTQDYCYRFAGYCLNVSRHTLERDGEPIKLTR
                     AEYEMLVAFVTNPGEILSRERLLRMLSARRVENPDLRTVDVLIRRLRHKLSADLLVTQ
                     HGEGYFLAADVC"
     gene            6568..7740
                     /gene="torC"
                     /locus_tag="OO357_003432"
     CDS             6568..7740
                     /gene="torC"
                     /locus_tag="OO357_003432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentaheme c-type cytochrome TorC"
                     /protein_id="HCR5888259.1"
                     /translation="MRKLWNALRRPSARWSVLALVAIGIVIGIALIVLPHVGIKVTST
                     TEFCVSCHSMQPVYEEYKQSVHFQNASGVRAECHDCHIPPDIPGMVKRKLEASNDIYQ
                     TFIAHSIDTPEKFEAKRAELAEREWARMKENNSATCRSCHNYDAMDHARQHPEAARQM
                     KVAAKDNQSCIDCHKGIAHQLPDMSSGFRKQFDELRASANDSGDTLYSIDIKPIYAAK
                     GDKEASGSLLPASEVKVLKRDGDWLQIEITGWTESAGRQRVLTQFPGKRIFVASIRGD
                     VQQQVKTLEKTTVADTNTEWSKLQATAWMKKGDMVNDIKPIWAYADSLYNGTCNECHG
                     APEIAHFDANGWIGTLNGMIGFTSLDKREERTLLKYLQMNASDTAGKAHGDKKEEK"
     gene            7740..10280
                     /gene="torA"
                     /locus_tag="OO357_003433"
                     /pseudo
     CDS             7740..10280
                     /gene="torA"
                     /locus_tag="OO357_003433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415517.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine-N-oxide reductase TorA"
     gene            10277..>10464
                     /gene="torD"
                     /locus_tag="OO357_003434"
     CDS             10277..>10464
                     /gene="torD"
                     /locus_tag="OO357_003434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415518.1"
                     /note="TorD; involved in the biogenesis of torA; acts on
                     torA before the insertion of the molybdenum cofactor and,
                     as a result, probably favors a conformation of the
                     apoenzyme that is competent for acquiring the cofactor;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone TorD"
                     /protein_id="HCR5888260.1"
                     /translation="MTTLTAQQIACVYAWLAQLFSRELDDEQLTQIASAQMAEWFSLL
                     KSEPPLTAAVNDLENSIAT"
BASE COUNT         2523 a         2685 c         2789 g         2467 t
ORIGIN      
        1 aaatgactgg tttagtaaaa tggtttaacg cagataaagg ttttggcttt atcactcctg
       61 atgatggcag caaagacgtt ttcgtccatt tcaccgccat ccagagcaat gaattccgca
      121 cgctgaacga aaatcagaaa gttgaatttt ctattgagca ggggcaacgt ggccccgcgg
      181 cagcgaacgt tgttacgctc taaggttgcc gttattactc aacatctcca tttccgctgt
      241 ccatgttgtc atggttcaca gtaccgtaca tcggcattcg atgtgacgga gcgaaaccct
      301 ttgggcgcta agtgtatttt ttgtaaatcg actatgatca cctttgataa cgtcgcgctg
      361 caaatacgca ctgaccatgc gccgctggat ttcacaaaat aatatcaggc tccctcgtgg
      421 agcctttttt atatctgcct tatttttctt caacgctgta tgtatagtaa gcgataacct
      481 gttgattatt gaatctttcg ggaagatggc ttataacatt tcttacctga ccagggtacc
      541 gggaaccaac accttactgg cgtgttgctg tctttcaaga ccagacgagg ttaacagtga
      601 atattgaaga gttaaaaaaa caagccgaaa cggaaatcgc cgactttatc gcgcaaaaaa
      661 tcgccgagct gaacaagaat acagggaaag aagtctctga aattcgcttc atcgcacgag
      721 aaaaaatgac cgggcttgaa agttatgatg tcaaaatcaa aataatgtga ttttgtgaac
      781 atcaccccgt gcgaggtgat gttccgcttg ttgctaattt agtgaccaat cattggcgct
      841 tgtggaatta agcgtcggta caattcctcc ggcaccgggc tttgccatac tcccgcatac
      901 attgcgtaac caatcaccgc aaacataatc cccagaacca gtagcgtcat taaccagcca
      961 gacaacgcaa aggctttttt atttgctgca ggtttttgca gtgaaaaggt caatgttgag
     1021 gctaccggac atgactctac gcaagtcata cagccggtac attccactgt tcgtacctga
     1081 attaatttat ctaccgggat ccgtgatggg cacttttttg cgcatttgcc acaatcgata
     1141 caactttcgg cattgcgacg aatcttaaac ggcgacaata gcgaaaccac gcccatcagc
     1201 gcgccatatg ggcaaagata acgacaccag gcatggcgaa taaacaggct ggcaatcagc
     1261 aaaacggtca cgctgattaa tgtcgcggtc cccatatgac gaaagaaatc gagcatttta
     1321 acgtccatca ccacgctata gggcgacaac ataaaatagt gaatcgcctg agcgggcatc
     1381 aataacgcga tatagagaaa taaactcaac agcaaatact tcacgccgcg cagaggaata
     1441 tccagccagc ggggaaggac acattgccga ccaaacagtt tgttaccgag atcgccgatt
     1501 aattcagaaa gcgtaccaac cgggcataac catgagcaaa aggccttttt gagtaataga
     1561 ctgatgacga taaaagcgac caataacagc atcgcggcgg cgtggacgga cggtaactga
     1621 cctgttgcaa ggctatattt cagattcatc agaccggcaa tcggtagcca gccttcgata
     1681 cctcccggtc tggcgacaaa tgtcgtacta cctgccgttt cgtaatagcg cacccaatac
     1741 cagaacgtga tggcaatata aatattcatt gccaatagta agaattgcgt cgctttacgc
     1801 caggtcgtgg cattgcgcca gtcattccac ggtaatttgc cgcccgtcgt gcctggccgc
     1861 cgctgccagc gggttctttt tttctctgcc atgattttgc cagtccgtta agttgcatac
     1921 caaatgccac tattctagtt attcttaact ggctgatatt gattcaaatc gcgttcaggt
     1981 ctttcttatt caatcatgct tccagagcgg caacactgcg tgtaatttct tcgtgtggaa
     2041 ggggggcaga taatggctgc tgctccagtt gtgcgcatag ctgactggcg ctacgcattc
     2101 ccagactcga gcaactgctt tttagctgat gcgcggcacg ctttattttc tcgctgtcct
     2161 ggctggcgcg ggcaatgtcg atttcatcga gaagcggcag ggcatgttgt ttaaataatg
     2221 ccagccattc gtggatcttc tccgtcccca ttaactgagc gtcttcattg agttgcgaga
     2281 catccagtgg tagatcgtta ttggcttgca gttggagata gtgcgccagt aactgaccga
     2341 gcacttcacg cggcaccggt ttagggataa tcccgcgaaa tagtgaactg gtacgctggc
     2401 gcagcgtttc gtcaatgaca tgggcgctaa agccaatcaa aaccagcgac ggatattgct
     2461 gtgccagttg ccgggcaagg gtaatgccgt cgatatccgg cagatcaaaa tccaccagtg
     2521 cggcagcaaa cggttcgcta ttttgcagtg tctctaaagc ctgcgcggca ttgccaatag
     2581 caacaacctg cgcaccactg gtgttcagca tctcaacggt aattcgctgg gttagcgggt
     2641 tatcttcaat taacagcaaa cgtaaaccgt caagacgcac cgcctgattg gctgtttttg
     2701 gcacgggtgc agtggcaaca cgtaacggca agcgtaaaca aaaacagctt ccaacctccg
     2761 gcgtgctggt ggcgctcagt tcgccgccca tcgcctgggc cagacggcta ctgatagtca
     2821 gccccagccc ggtgccgccg cgtttgccgc ttacctgcac aaatggctgg aagatttctg
     2881 ccagtttcgc gggatcaata ccgcagccgc tgtcttccac ttcgaccagc cattgctcgc
     2941 catcagtgcg actacgcagg ataatgtgcc cttcgtcagt aaaacgcagg gcattgctca
     3001 ataggttggt aatgacctga cgaatacgtc gtggatcgcc cattaacgcg ctcggtacat
     3061 catcggcaat tgccgttgcc aggcgaatcg ggcgaccttt cacccgtccg ctcattaatt
     3121 gcagggtact ttccagcagc gggcgcggtt caaagggctc atcgctgact gaaacatttt
     3181 tgccacctgc ttcgatagcc gaataatcga gaatatcgtt gaggatggtc agcaacgatt
     3241 cgccagagtc ggtaatcgcc tgcaaatcat cacgctgggc gttaagtgcg gggttatctg
     3301 ccagcagttg agcagtgccg agaataccgt acagcggtgt gcggatctca tggctcatcg
     3361 ccgtcagaaa cgccgatttt gcctggctgg ctttttctgc ttccgcccgt gcctgtcggt
     3421 gttctatcac cagttcctgc aattcagctg tacgcgcttt gacctgcgcc gccagctgtt
     3481 cgcggtggcg attcagtgca tgaacactgc tgcgaaacgc atccatcagc cgcccgatgg
     3541 tatccagctc ccgtacgcca gcggtttccg ggaaagggga gtcaatatca ccgtccagca
     3601 gccgttgcag cgcctgcgtt tgttcggcaa gtggacgcgt gaatgagcga taaaccacgc
     3661 gccagaggat cataatcagt gcgcaaagtg aaaccatccc cagcaataac aggctgtatt
     3721 gcccgcgtgc actggctttt tccagatgtg ccagtccatg ctgattgtgc agctcaatgg
     3781 tgtcgaccag ctgactgact tcgctactaa actgcgcgaa ctgggcgata ttattttggg
     3841 cgagagtttg caggtgattg ctgatttcac tgtcctgctg gaacagcgcc aacagatcgc
     3901 tatattggct aacggtagtt aacgttgttg cgacctgcgc acgaactccc ggatcttcaa
     3961 tgcgtatttg ccgacgttgc agaattttca ccgcattatt gagctgcttt tccagcgttg
     4021 gtgcattttt ctggatctgc tccagcccca gattcatcac catttgctgc acccgcagag
     4081 cgctaaggcg cagttcattc atctggttaa catactcaag atcgatatca atcagccgat
     4141 cgagtgcact ttcagcagcc tgacgctgat cttgttcgat caaatcgtaa atcccggcct
     4201 gggtcgctcc agcggaagtt gtcgcattat tcgcctgacc ttgctccagg cgtgcgatct
     4261 catcggcggc agctatctgc tggctgagtt gccgttgttg ctggcgtagt tgcagacgcc
     4321 gccccaccag ttccccttgc tgacgtaatg aacgggagat ctcctgctcc tgttgttcaa
     4381 tagcggtggt atcaaaacct tgttcccgta acgcttgcag caacgcatta atcttcaggc
     4441 tttatgcggt gagcattcgc ccctgcgcct gccacatctt ttcgttatcg gcactggtca
     4501 ggttctgcgc ggcgaaaagt tcccaggcgc tggcttcgct caactggcgc gccatattca
     4561 tggtaggaat caatgcctga gtattgtctt tttccacctg gctgataaag cgcaggttgt
     4621 accatcccac cagggtactg gtcagggtta acagcgccat cagggcaaag cccatccaga
     4681 gtcttcgggt cagggttaaa ttcacggtcg gtgcacttaa ggtgaaaaag gttgagtcgc
     4741 aaagtggaat gcatctagca taaagcctta ttattgatga ggctatcatg cgcgtactgc
     4801 tatttttact tctttccctt ttcatgttgt cggcattttc ggctgataac ctgttgcgct
     4861 ggcatgatgc gcagcatttc acggtgcaag cctctatgcc gcttaaagcc aaacgcgtat
     4921 ggaaactgtg cgcgctttat cccagcctga aagattcata ctggttatcg ttgaactatg
     4981 gtatgcagga ggctgctcgc cgctacggtg tggatttaaa agtgctggag gcaggcggct
     5041 acagccagtt ggctacccag caagcacaaa tcgaccagtg taaacagtgg ggcgcagagg
     5101 ccattttgct cggtagtagc acgacgtcat ttcccgacct gcaaaagcag gtagcaaatc
     5161 tgccggtgat cgaactggta aatgctcttg atgatcccca ggtgaaaagc cgcgttggtg
     5221 taccctggtt tcagatgggc tatcaacctg ggcgatattt ggtgcaatgg gcgcacggta
     5281 aaccactgaa cgtgctgctg atgcccggac ccgataacgc cgggggcagt aaggagatgg
     5341 tcgagggttt tcgcgcagcc attgccggaa gcccggtgcg tattgttgat attgcgcttg
     5401 gtgataacga tattgaaatc cagcgcaacc tgttgcagga gatgctggaa cgccacccag
     5461 aaatcgacgt cgttgccgga acggccattg cggcagaggc ggcaatgggt gaagggcgta
     5521 acctgaaaac gccgcttacc gtggtgtcgt tttatctttc acatcaggtg tatcgcgggc
     5581 tgaagcgggg aagagtgatt atggctgcca gcgatcaaat ggtctggcag ggggaactgg
     5641 cagttgagca ggccatcagg caattacagg ggcaatcggt gtctgataat gtcagcccac
     5701 cgattttagt tctgacgccg aaaaatgccg accgagaaca tatccgccgc tcgctgtcac
     5761 cggggggatt tcgtccggtc tatttttatc agcacacatc agcggctaag aaataacctt
     5821 caccatgttg cgtcaccagt aaatccgcgc tgagtttatg acgtaaacga cgaattaaca
     5881 catcgacggt gcgcaggtca gggttttcca cccgacgcgc agaaagcata cgcagcagac
     5941 gctcacggct gagaatttcg cccggattag tcacaaatgc caccaacatt tcatactctg
     6001 cgcgggtcag tttaatcggc tcgccatccc gctccagcgt atggcgcgac acattcaggc
     6061 aataacccgc aaagcgatag cagtagtcct gagtgtgcgg ttgagcttgt cgcgcgaggt
     6121 cgattcgcca gagcagattt ttcacccgta ctaccagttc gcgcagttcc agcggtttgg
     6181 tgacgtaatc gtctgcgccc atttccagcc caacaatacg gtcaatccga tcgctgcgtc
     6241 cggtaaccag aataatcccc accgttgagc gttctcgcag ggcgcgggtt aacatcaggc
     6301 cattttcatc gggtaagttg atatccagca gaattaaatc taccggctga ttctgcataa
     6361 tttcccgtag cccggcaccg ctcgccgtaa cggaaacggt atacccctcc tgagtgaagt
     6421 aggattgtaa tcgcgcctgg gtaaccggct tatcttcaac aataacaatg tgatgtggca
     6481 tcagagggtt ttactcattc tgttcatatc tgttcatatt tgctcattaa gatcgcttca
     6541 ctaaaccata attctacagg ggttattatg cggaaactct ggaacgcgct acgccgaccc
     6601 agtgctcgtt ggtcggtact ggcgctggtc gcaattggga ttgtgattgg cattgcgctg
     6661 attgtattgc cacacgttgg gatcaaagtc accagcacaa ccgaattttg tgtcagttgc
     6721 cacagtatgc aaccggtgta tgaagaatat aaacagtcgg tgcatttcca gaacgcctcc
     6781 ggcgtgcgag ctgaatgcca tgactgtcat atcccgccgg atattccagg catggtgaag
     6841 cgcaaactgg aagcgagcaa cgacatttac cagaccttta ttgcccactc cattgataca
     6901 cctgaaaaat tcgaagccaa acgcgcggaa cttgccgagc gtgaatgggc gcgaatgaaa
     6961 gaaaacaact cggcaacctg tcgctcctgc cataactacg atgcgatgga tcatgccagg
     7021 cagcatcctg aagcggcacg tcagatgaag gtggcagcga aagataatca atcctgcatc
     7081 gactgtcata aaggtattgc ccaccagtta ccggatatga gtagcggctt ccgtaagcag
     7141 ttcgatgagc tgcgcgccag tgctaatgac agtggtgaca cgctgtactc tattgatatt
     7201 aagccaattt atgcggcgaa aggcgataaa gaagcctctg gttctctgct gcctgcttcg
     7261 gaagtgaaag tccttaaacg tgacggcgac tggctgcaaa ttgaaattac cggctggacg
     7321 gaaagcgccg gacgtcagcg tgtactcacc caattcccag gtaaacgcat ctttgttgcc
     7381 tcgattcgtg gtgatgtgca gcagcaggtg aaaacgctgg agaaaaccac cgttgccgac
     7441 accaataccg agtggagcaa gttgcaggcc accgcgtgga tgaagaaagg cgacatggtg
     7501 aacgatatca aaccgatctg ggcctatgcg gattcgttgt acaacggcac ctgtaacgag
     7561 tgccacggcg caccggaaat cgcccacttt gacgctaacg gttggatcgg cacgctcaac
     7621 ggcatgattg gctttaccag tctcgataaa cgtgaagaac gcactttgtt gaaatatctg
     7681 caaatgaatg cgtctgacac cgcaggtaag gctcacggcg ataagaagga agaaaaataa
     7741 tgaacaataa cgatctcttt caggcatcac gtcggcgttt tctggcacaa ctcggcggct
     7801 taaccgtcgc cgggatgctg gggccgtcat tgttaacgcc gcgccgtgca actgcggcgc
     7861 aagcggcgac tgaggctgtc atctcgaaag agggcattct taccgggtcg cactgggggg
     7921 ctatccgcgc gatggtgaag gatggtcgct ttgtggcggc aaaaccgttc gaactggata
     7981 aatatccgtc gaaaatgatc gccggattgc cggatcacgt acacaacgcg gcacgtattc
     8041 gttatccgat ggtacgcgtg gactggctgc gtaagcgcca tctgagcgac acctcccagc
     8101 gcggtgataa ccgttttgtg cgcgtgagct gggatgaagc cctcgacatg ttctatgaag
     8161 aactggaacg cgtacagaaa actcacgggc cgagtgcctt gctgaccgcc agtggttggc
     8221 aatcgacggg gatgttccat aacgcttcgg ggatgctggc gaaagctatt gccttgcatg
     8281 gtaatagcgt tggtacgggc ggagattact ctaccggtgc tgcgcaggtg atcctgccgc
     8341 gcgtagtcgg ttcaatggaa gtgtatgaac agcaaacctc ctggccgctg gtattgcaga
     8401 acagcaaaac cattgtgctg tggggctccg atttgctgaa aaaccagcaa gcgaactggt
     8461 ggtgcccgga tcacgatgtt tatgaatatt acgagcagct aaaagcgaaa gtcgccgccg
     8521 gtgaagttga ggtcatcagc atcgatccgg ttgtcacatc cacccatgag tatctggggc
     8581 gcgagcatgt gaagcacatt gcggttaacc cgcaaactga cgtgccgctg caactggcgc
     8641 tggcacatac gctgtacagt gaaaacctgt acgacaaaaa cttcctcgct aactactgtg
     8701 tgggttttga gcagttcctg ccgtatctgc tgggtgagaa agacggtcag ccgaaagatg
     8761 ccgcatgggc tgaaaaactg accggcattg atgccgaaac cattcgtggg ctggcgcggc
     8821 agatggcggc gaacagaacg caaattattg ctggctggtg cgtgcagcgt atgcagcacg
     8881 gtgaacagtg ggcgtggatg attgtggttc tggcggcgat gctggggcaa attggcctgc
     8941 caggtggtgg ttttggctgg cactacaacg gcgcaggcac gccggggcgt aaaggcgtta
     9001 ttctgagtgg tttctccggc tctacgtcga ttccgcctgt tcacgacaac agtgactaca
     9061 aaggctacag cagcactatt ccgattgccc gttttatcga tgcgatcctc gaaccgggga
     9121 aagtgatcaa ctggaacggt aaatcggtaa aactgccgcc gctgaaaatg tgtatttttg
     9181 ccggaactaa cccattccat cgccatcagc agatcaaccg cattattgaa ggctggcgca
     9241 agctggaaac ggttatcgcc atagataacc agtggacctc aacctgccgc tttgccgata
     9301 tcgtgctgcc tgcgaccacg cagtttgagc gtaacgatct cgaccagtac ggcaaccact
     9361 ccaaccgtgg cattatcgcc atgaaacagg tggtgccgcc gtagttcgag gcgcgcaacg
     9421 actttgatat tttccgcgag ctgtgccgtc gctttaatcg tgaagaagcc tttaccgaag
     9481 ggctggacga aatgggctgg ctgaaacgca tctggcagga aggtgtacaa caaggcaaag
     9541 gacgcggcgt tcatctgcca gcgtttgatg acttctggaa taacaaagag tacgtcgagt
     9601 ttgaccatcc gcagatgttt gttcgccacc aggcattccg cgaagatccg gatctcgaac
     9661 cgctgggcac gccgagtggc ctgattgaga tctactcgaa aaccatcgcc gatatgaact
     9721 acgacgattg tcaggggcat ccgatgtggt ttgagaaaat cgaacgctcc cacggtgggc
     9781 ccggctcgca gaagtatccg ttgcatctgc aatctgtgca tccggatttc cgacttcact
     9841 cgcagctatg tgagtcggaa acgctgcgtc agcaatatac ggtagcgggt aaagagccag
     9901 tgttcattaa cccgcaggat gccagcgcgc gcggtattcg taacggtgat gtggtacgcg
     9961 tctttaacgc tcgcggtcag gtgttggcag gagcagtagt ttctgaccgc tatgcacccg
    10021 gcgtggcgcg aattcacgaa ggggcatggt acgatccaga taaaggcggc gagcctggtg
    10081 cgctgtgcaa atacggtaac cccaacgtgc tgaccatcga catcgggact tcgcagctgg
    10141 cgcaggcgac cagtgcgcac actacgctgg tggaaattga gaagtacaat ggaacagtgg
    10201 agcaggtgac ggcgtttaac ggccccgtgg agatggtggc gcagtgcgaa tatgttcccg
    10261 cgtcgcaggt gaaatcatga ccacgctgac agcacaacag attgcctgtg tttacgcctg
    10321 gctggcgcag ttgttctccc gtgaactgga cgatgaacaa ctgacgcaaa tcgccagtgc
    10381 gcagatggct gaatggtttt cgttgctgaa aagcgaaccg ccgctcactg cggcggtgaa
    10441 tgatctggaa aacagtattg ccac
//
LOCUS       DAJTRC010000120        10435 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.120, whole genome shotgun sequence.
ACCESSION   DAJTRC010000120 DAJTRC010000000
VERSION     DAJTRC010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10435)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10435)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10435
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.120"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..272)
                     /locus_tag="OO357_003435"
     CDS             complement(<1..272)
                     /locus_tag="OO357_003435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001300094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5888261.1"
                     /translation="MYRAAATHPKIKLLQFKLIPCPGMNKAIPSLIADFTGELHRLIS
                     EHLQCKRRKDKSVEPTPLYYLWSREAVANDATPYLMVLLLNNDVWC"
     gene            complement(366..928)
                     /locus_tag="OO357_003436"
                     /pseudo
     CDS             complement(366..928)
                     /locus_tag="OO357_003436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004714555.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus Gp2 family protein"
     gene            complement(1132..1338)
                     /locus_tag="OO357_003437"
     CDS             complement(1132..1338)
                     /locus_tag="OO357_003437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001444904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HCR5888262.1"
                     /translation="MVYKSDSDELAVLRAENTRLVSLLEAHGIEWRRKPQSPVQCVFV
                     LSTDEKVALFRRLFRGRDDVWALR"
     gene            complement(1445..2008)
                     /locus_tag="OO357_003438"
     CDS             complement(1445..2008)
                     /locus_tag="OO357_003438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5888263.1"
                     /translation="MYNANPNYKMDFMILKDVNEHMDGMFQRFSKLLPFRIDFAYRKD
                     TPSFGHSCKHSMCMEIYRLLSETQTMLAGYYWVMEYTPDKGLHIHFIGYLDGQRHKNS
                     YQISRQLGDIWRRITEGEGYFHLCRAKDKYPVRIDHVIHYSDKSAVDDLRYALSYLAK
                     QDQKEHGIILGRSRLPEKSNRGRPRHN"
     gene            complement(3383..5218)
                     /locus_tag="OO357_003439"
     CDS             complement(3383..5218)
                     /locus_tag="OO357_003439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001258873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888264.1"
                     /translation="MRTFTNMLYDICTVLGLFKEGENPAHKRKSTNFEMHQKFWDQRY
                     NEISRIIDAEGVFSQEQRRIIYARYEHFYYMMNSYPVHSTLKPEFLRSYCLRTFGVIF
                     LVVDMYNTYRPENDSAFYYHIYNFLQKSYCPCLDHADTESDEAAVKRYLREYLAELGF
                     NKEDFHENGKLYALGKYTGTIRKDNGKSKSLMQQYIMAIKNEYKKDYREKKLDKDELE
                     KVLRNIDKFYNAFYSLSVLLDIQRKTKILQSLAYYLRVLVREGLWIHGLYGYAAQYLY
                     DFTSFDTTPYAKKLLEMFYKFQNSAEGTLSRYSVSLDDKSQEYISRLKDLVFNINDKN
                     GCDDAYLKKIISYFGQLQNEAVHVTSCYETLAVYICLIRKNKINDVLQHYDDMERKGL
                     FGELPSGYVRGALSLLRTALEVKVNRKNIKYGSLFYWLDHVKAYQDAFIEKIPLIDPV
                     YKEGEIQYDANNFTLMRVIKMYNCMLEKISTKPYIAPPYITGLLDDVEKVLDKINILI
                     DKEYVYDGKTLAEVIMENKVLSSRERKETMIGLFTGSKKYTLLQCVEKLGVLVHYVKS
                     PVDEIKNVMMLYGDKAENRNRRRMIYDALTIICEDDIRNNPPELS"
     gene            5319..5606
                     /locus_tag="OO357_003440"
     CDS             5319..5606
                     /locus_tag="OO357_003440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000070930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlpA family transcriptional regulator"
                     /protein_id="HCR5888265.1"
                     /translation="MSNQTGQLYTPSRILRMAELTRLLGISRSTIYVKINPASKYHDP
                     TFPKPIRLGSASVGWRASAIDEWLMLHTAPAWSEPENKLKELNDDDKTITP"
     gene            5578..7107
                     /locus_tag="OO357_003441"
     CDS             5578..7107
                     /locus_tag="OO357_003441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016231106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HCR5888266.1"
                     /translation="MMTTKLSLPELDTTKLPDRCQALLDEMHEETGIRREILLSVMLT
                     VMAASVQDTHEVELSGGQRTSLQIFMCLSSASGSGKTSACAKLIAPVHETEEELHQAY
                     IDDKKNYDRMMEMWTTDKKILERRYKKEIERSPENAAAARAALEKCIANKPEPPVQQV
                     LIVNDATPEGIALKLSQSPSLLLLSDEGGTILDKRFERKSALYNTLWSGQPVNVERAS
                     RPGFRLNDVRLTILILTQPVIFNKFFTLTGDQIRGNGFLARLLFCEPGAIKIMTTEPH
                     TATPVVTQQCASCFRKKSFGSQIRDSLRASRERRAKSKERICMTLSNAASRALERFHK
                     ENMDTVRQNPHMGTFEDIIVRKREQVVRIAALLELEKDPDSTVITLESTNSAIYLIEF
                     YFKHLIYKLESLREISPAEKLDKWLQKRIITTAGYIFQKSYILQYAPYALRKKCVLDE
                     ALDILAEQRKIRIDDNLVVYIGNTITPSELAKKLNIPAFDAGVFICDHQNILKYHRNR
                     L"
     gene            complement(7277..8494)
                     /locus_tag="OO357_003442"
     CDS             complement(7277..8494)
                     /locus_tag="OO357_003442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000279856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5888267.1"
                     /translation="MAVLTDTKARHIKPDDKPLPHGGITGLTLHPSSVKGRGKWVFRY
                     VSPVTQKRRNAGLGTYPEVSIAEAARTARIMREQLAAGDDPLEIKKAESEKVVIPTFA
                     DAARRVHAELSPGWENPKHVRQWLSTLENYAFPQLGAKTLDSITAADVAETLRPVWLT
                     LSETASRVKQRIHVVMQWGWAHGFCVANPVDVVDHLLPQQTRGRDEHQPAMPWRQLPL
                     FVATSVYSDEPYNVTRALLLMVILTATRSGEARGMRWAEIDFHKRVWTIPAERMKARL
                     QHRVPLSRQAIYILENIRGLHDELVFPSPRKQQILSDMVLTSFLRKKKAVSDIPGRVA
                     TAHGFRSTFRDWCSEQGYSRDLAERALAHTLKNKVEAAYHRTDLLEQRVPMMQAWADY
                     VMSQLAKNTKEFA"
     gene            complement(8861..9847)
                     /locus_tag="OO357_003443"
     CDS             complement(8861..9847)
                     /locus_tag="OO357_003443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888268.1"
                     /translation="MIPDYLTFIRFQDKRNLIYIYAIGLILIGFYWKNAGFTFPSEDI
                     GVVSGILALVLYNFIFDLKAYWAYKCVTKNIDFSWFKKKQNHKIELFLTQPLVAGFLS
                     LIMLSAMSWGLYQLLPSLYALFLISLLGPLVIFLLFRMIRTSYVKQVAISVAKKVKYK
                     SLTRYVLLSVCISTVVNLLTISPLRNSDSFVTEGQWLTFKSIIALLILCGVVLAINLF
                     FLRFSKRPAFLGRFFLQEIDLFFSSENTLSTFFAKPLWLRLFILRVIEMMWITLVSVL
                     ATLVEWRIWFEAYFLLCYVPCLIYYFFHCRFLWHNDFMMACDMYFRWGHFNK"
     gene            complement(9844..10372)
                     /locus_tag="OO357_003444"
                     /pseudo
     CDS             complement(9844..10372)
                     /locus_tag="OO357_003444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309324.2"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
BASE COUNT         3249 a         2386 c         1965 g         2835 t
ORIGIN      
        1 ttacaccaga catcgttatt aagtagcagc accataagat aaggagtggc atcgttagcc
       61 acagcttccc tactccagag gtaatataaa ggggtgggct cgactgattt atctttacgt
      121 cgcttacact gcaaatgttc agaaattagt ctatgcagct caccagtaaa atcagcaatc
      181 agagaaggaa ttgccttatt cataccaggg caaggtatta atttaaactg taataattta
      241 attttaggat gtgtagcagc agcccgatac agagttgcaa ggacacactt ttgccagagg
      301 gcgttacagg aaagcttaat gtttgattct gtatacataa taaatcacct tatagttaca
      361 ataggtcaaa aaccgctgta gccaaagtta cgctggcctg atgctttagt gtcgagcttc
      421 gtcaaataat ccagacgtac caataagcgc tggtactgct ctgggaaatt aggatcgtta
      481 atatccagga tgtaacgtcc attagcaggg aaatgaacta atccctggtg atcttccagt
      541 tccaacccta aggcactgta ccaggctccg gtaattaacc ctctcagggt gtcagagcgc
      601 tcatagtttc ccagatggta ataggcgtct ttgttaaaaa aaggcagacg tgataatgac
      661 atttaaagga ctgcgtatat tctttcgccc agataataca aaccgacgtt gcgtaggtgc
      721 gtttgccttg ttgtttttta cggtgttgat ctgcattaat tttcgcattc agcgagttac
      781 ggaaacgaga tatctctccg gggatcaggt tagggaaaca ggtaatgata tccccgttgt
      841 caatcacagg agggtaatgg agatcccccc gtattgctgt tattctggaa gtgtttttaa
      901 tattcgtatt gatagttctc agagatatat cacactctag atattttaat taagtatcag
      961 ttcgatatcg gcatattgta cactacctca ggtagatgat gagatcagat acagggatca
     1021 actgacggtg agcgcagtcc ccacatttta tccagggctt actgcatatt cccaacagcc
     1081 attcgttagc caggtagacg aataccctga tttgccgctg gttttgcttt cctatcgaag
     1141 ggcccataca tcatcacgcc cacgaaacaa ccgacgaaat aacgcaactt tctcatccgt
     1201 ggataatacg aaaacgcact gcacagggct ctgcggttta cgtcgccatt caatcccatg
     1261 agcttcaagt aatgagacca gccgggtatt ttctgcccgc agtactgcta gttcatcgct
     1321 gtcagattta taaaccatat gcgttatcac ttgtacctgt ctcccggcgg caccacgact
     1381 gaaattcgtg tgaaggcaaa tccacgcgat aacttacgat agttcggaca taagccccca
     1441 taattcagtt atgtcgtggt cgaccccggt tacttttttc gggaagcctg ctacggccca
     1501 gaataatacc gtgttctttc tgatcctgtt tagccagata gctaagggcg taacgtaagt
     1561 cgtctaccgc agatttatca ctatagtgaa tcacgtgatc gatacgcacc gggtatttgt
     1621 ctttagcccg gcacagatga aaataaccct ccccttccgt gatccgcctc cagatatctc
     1681 ccagttgcct ggatatctga taagagttct tgtgacgttg tccatccagg tagccgataa
     1741 aatgaatatg aagccctttg tctggtgtat attccattac ccagtaatat cccgccaaca
     1801 tcgtctgggt ttcgctaagt aaacggtata tttccataca catactgtgc ttacaggaat
     1861 gcccgaaact gggcgtgtct ttcctgtaag caaaatcaat tctgaaaggt aacagtttag
     1921 aaaaacgttg aaacatacca tccatgtgtt cattcacgtc tttcagaatc atgaaatcca
     1981 ttttgtaatt gggattggca ttatacattt tatagtcctt actttataaa actcacagga
     2041 agagagaatt acgtaaatac tgagagtaga gaaacgccgt accacggcat taataaacga
     2101 agtaaaccag cagcacagac aacctgataa aactgttttg tattaccgca ttactgtgat
     2161 gaggtaatac ttcagaatac agtgagactc tcttaaactg agaaccagta cgaattacaa
     2221 tggaaaaaca cacctcatag aattgagttt caggaagcac cactaaattc tttacaggaa
     2281 gtaatctgac attcacaaaa ctcagtatcg gctagctaca ggcaacatcg ctattaatat
     2341 tgtcgaacca ttaagtaaag ccgagagaga ccacgggtat ttaatcatta gcataaaaga
     2401 ataatggtat acagaacata cccacacacc ataagaagat tgttccatac agaaaagatc
     2461 atatttacct atacctataa actgtaacgg agtcataaca cgctctaata gtgttccgat
     2521 acgtaaccag tgttaagtac gtacaggaca acattacaca cataattcac catacagctt
     2581 cccccatatg cttcactgcg tagtgtatgg agatatatgc attacacaca tacagaatac
     2641 ttagcataaa gaggagtgac attaccacgt aaagagataa gttatacaac aaacatacaa
     2701 acaatacggc acagacacac aataccataa ctccaactaa taatactaat taaccttaat
     2761 tatttatggt atatgaaata aatgtgattc tcccggaact aactaaccta accagacaga
     2821 tgatcactgt ataaaaactt cagttaatac tcatttgttt tgtcgactca gtatataatg
     2881 agtgaccagt aatcaggata atcacactta cataacctgt aaaaaagaac aagaagaata
     2941 agccactaac ataatgcaaa atggcatctg aatcgatcgc agtttggcat tcgaatcgat
     3001 catgatttgg cccaatccgt gatcgaatag agcggaatac gcaacataat tcaccagaca
     3061 agagcttaca ccgaattaca gattatgtat taatgtcaaa actggcatat tttacagata
     3121 caatgaacat gcaacccatc aatctaatag gggcggtatg tacatctgcc catgaatggg
     3181 atggcgctaa gaatacaaaa caattagaca tagatcacaa aaacacacca ttctaattga
     3241 aaaacaaaac aaacaaataa atcatactat tatagaattt tctcttgcca gacatagata
     3301 atacccctgc taattttgct aatggttagc aaaattagca gggtatataa agattattga
     3361 cccaaaacac actcagatga attcacgaca gctcaggagg gttattcctg atgtcatctt
     3421 cacaaataat ggttaacgca tcatatatca ttctgcgtct gttcctgttt tcggccttat
     3481 cgccatacaa catcatcaca tttttaattt catcgaccgg tgatttcaca tagtgaacta
     3541 atacaccaag cttctcaaca cattgcagta aagtatattt cttactgcct gtgaaaagcc
     3601 ctatcatagt ttctttacgt tcgcgactgg ataatacttt gttttccata atgacttctg
     3661 ccagcgtctt accatcataa acatactctt tatctatcag aatatttatc ttatccagca
     3721 ccttctctac gtcatcaagc aaacctgtaa tatatggggg cgcaatatat ggtttcgtac
     3781 tgatcttttc aagcatacaa ttatacatct taataacacg catcagggta aaattgttcg
     3841 catcatactg tatttcaccc tccttatata caggatcaat aagtggtatt ttttcaataa
     3901 atgcatcctg ataggctttt acatggtcaa gccaataaaa caacgagcca tactttatat
     3961 tttttctatt tactttaacc tccagtgctg tccgaagcaa cgataatgcc cccctgacat
     4021 atcctgaagg taattcacca aacaatcctt ttctttccat gtcatcataa tgctgcagca
     4081 catcattaat tttatttttc cttatcagac aaatgtacac cgcaagtgtt tcgtagcagc
     4141 tggtcacatg aacagcctca ttctgcaact gcccaaaata gcttataatc tttttcagat
     4201 atgcgtcatc acaaccattc ttatcattaa tattaaatac aagatctttc agacgggaaa
     4261 tatattcctg agatttatca tcaagtgaaa cactataacg agacaatgtt ccttctgcac
     4321 tgttttgaaa cttataaaac atttccagta atttttttgc ataaggcgtt gtatcaaagc
     4381 tggtaaagtc ataaagatat tgcgcagcgt aaccatacaa accatgaatc cagagtcctt
     4441 ctctcaccaa cactctgaga taatatgcaa ggctttgaag tatcttcgtc tttcgttgta
     4501 tatcaagtaa gacacttaat gaataaaatg cattatagaa tttatcaata ttcctgagca
     4561 ctttctccag ttcatcctta tccagcttct tctcccggta atccttttta tattcatttt
     4621 tgatagccat gatatattgt tgcatcaggc tcttgctttt cccgttatcc ttccgtatag
     4681 ttcctgtata tttccccaga gcatacagct ttccattttc atgaaaatct tccttgttga
     4741 atcccagttc agccagatat tcccgaagat aacgtttaac agcagcttca tcgctttctg
     4801 tatcagcgtg atcaagacaa ggacaatacg atttctgtaa aaaattataa atatgataat
     4861 aaaaagcaga gtcattctct ggtctgtatg tattatacat atctacaaca agaaaaataa
     4921 ccccaaaagt gcgcagacaa tagctgcgaa ggaattcagg ttttagtgtg gaatgcactg
     4981 gataagaatt catcatgtaa taaaaatgtt catatcgggc atatatgatc cgccgttgct
     5041 cctgactgaa caccccttca gcatcaataa tacgactgat ctcattataa cgttgatccc
     5101 agaatttttg atgcatttca aaattggtac tcttccgctt gtgtgccgga ttctcgcctt
     5161 ccttaaacaa ccccaaaaca gtacaaatat catacaacat atttgtaaat gtgcgcatat
     5221 gctgtgctct ccggttggtt tgcattttat cgcagaggac attctgagct taacgtatca
     5281 aactgacaca acgacatgca atgcatatgg agaacaatat gtcaaatcaa accggacagc
     5341 tttacacgcc ttcacgcatc ctgcgtatgg ctgaacttac ccgactgctg ggtatatcaa
     5401 gatctactat ttacgtgaaa ataaatccgg cctcgaaata ccacgaccct acctttccca
     5461 aaccaataag gcttggtagt gcatccgtag gctggcgggc atctgccatc gatgagtggc
     5521 tgatgttaca tacggcacca gcctggtctg aacctgaaaa caaactaaag gaacttaatg
     5581 atgacgacaa aactatcact ccctgaactt gacacaacta aactgcctga caggtgccag
     5641 gcattacttg atgaaatgca tgaagaaaca ggaatacgcc gtgaaatact tctgtctgtt
     5701 atgctgacgg tcatggctgc ctcggtccag gatacgcatg aagttgaact ttccggaggg
     5761 caacgtacca gccttcagat atttatgtgt ctttcatcag catcaggcag tggaaaaacc
     5821 tctgcctgcg caaaattaat cgctcctgtt catgaaacag aagaagaact acatcaggca
     5881 tatatcgacg ataaaaaaaa ctatgaccga atgatggaaa tgtggacaac agataaaaaa
     5941 atcctggagc ggagatataa aaaggaaatt gaaagatccc cggaaaatgc tgctgctgca
     6001 cgagcagcac ttgaaaagtg tatagcaaat aaacccgaac ctcccgtaca acaggttctc
     6061 atcgtgaatg acgcgacacc agaagggata gctttgaaac tcagccagtc accctctctt
     6121 ctgttgctgt ctgatgaagg aggtactatt ctggataagc gtttcgaacg caaatcggca
     6181 ctgtacaaca cattatggag tgggcaacca gtcaacgttg aacgggcatc cagacccggg
     6241 ttccggctga atgatgtccg tctcactata cttatactga ctcaaccggt aatatttaat
     6301 aagtttttta ctctcactgg cgatcagatc cggggcaatg gctttctggc ccggttgcta
     6361 ttttgcgaac ccggagcaat taaaataatg acgacagaac cgcatactgc cactcctgtt
     6421 gtgacgcaac aatgtgcaag ctgttttcgt aagaaaagtt ttggcagtca gatcagagac
     6481 tctctgagag catccagaga aagacgtgca aaaagtaaag agcgcatctg catgacatta
     6541 tcaaatgcag catcccgcgc actggagcgc tttcacaaag aaaatatgga tactgtcaga
     6601 cagaatcctc atatgggtac atttgaagac attattgtca gaaaaagaga gcaggttgtc
     6661 aggatagcag cacttctgga actggagaaa gatccagact cgacagtaat aacgctggaa
     6721 agtactaata gcgccattta tctcattgaa ttttatttca agcatcttat atataaactt
     6781 gaatcccttc gggaaatatc cccggctgag aaacttgata aatggcttca gaaaagaatt
     6841 atcacaacag caggttacat attccagaaa agttatattt tacaatatgc cccatatgct
     6901 ctcaggaaaa aatgtgttct ggatgaagca cttgacatac tggcggaaca aaggaaaata
     6961 aggattgatg ataacctggt tgtttatata ggaaacacga ttacaccatc cgaactggca
     7021 aaaaaactaa atattcctgc attcgatgcc ggagttttca tttgtgatca tcaaaacata
     7081 ctgaagtatc accgaaacag actatagata ctatcggaca aaaaataaat tatcacaagt
     7141 gtattattcg ttataataat gaataatcgt tatctgagtg caatgccagg ttttcattac
     7201 gaactggcat tgcatttatt tttcaaaaaa gaaagcagta accatttggc tactctgagt
     7261 atgccagtcc atgaaactaa gcgaattcct tagtgttttt agcaagttga gacatcacat
     7321 aatccgccca tgcctgcatc atcggtacac gctgctccag tagatcagta cgatgatatg
     7381 ccgcctcaac cttatttttc agcgtatgag cgagcgccct ttccgccaga tcccgcgaat
     7441 acccctgttc actacaccag tccctgaatg ttgagcgaaa accatgtgcc gtggcaactc
     7501 gccccggaat gtcactgacg gctttctttt tacgcagaaa acttgtcaac accatatcgg
     7561 aaaggatctg ctgctttctg ggtgaaggga acaccagttc atcatgcagg ccacgtatat
     7621 tttccagaat gtaaatagcc tgccgggata aaggaacacg atgctgtagc ctagctttca
     7681 ttctttctgc aggtatagtc catacccgct tatgaaaatc aatttcagcc cagcgcattc
     7741 ccctggcttc gcccgagcga gttgctgtaa gtatcaccat taataacagt gcgcgggtaa
     7801 cattataagg ttcatcacta tacacactgg tcgccacaaa aagcggtaac tgcctccagg
     7861 gcattgcggg ttggtgttca tcacgtcctc ttgtctgctg aggaagcaaa tggtcaacca
     7921 catcaacagg atttgccaca caaaaaccgt gcgcccatcc ccactgcata acaacatgaa
     7981 tgcgctgttt aacccggctt gccgtttctg acaaggttaa ccagactgga cgcagtgttt
     8041 ctgccacgtc cgcagccgta atcgaatcca gcgtttttgc tcccagttga ggaaacgcgt
     8101 aattctcaag cgtcgataac cactgcctta catgctttgg attctcccat ccaggagaca
     8161 gttctgcatg tacacgcctg gctgcatcgg caaatgttgg gataacgact ttctcagatt
     8221 cagccttttt aatctccaga ggatcatcac ctgcagcaag ttgctctcgc attatccggg
     8281 cagtacgtgc agcttcagca atactgacct ctgggtaagt tcccaatcca gcattacgtc
     8341 ttttttgtgt caccggactt acataacgaa aaacccattt cccccgcccc tttactgaag
     8401 aaggatgaag ggtcagtccg gtaattcccc catggggcaa tggtttgtca tcaggtttga
     8461 tatgtcttgc tttcgtatcc gtcaatactg ccatacgcta attcccgtca ctctggtatg
     8521 ccatccagta tgccattagt ccctcgcttc actaagaata cattagataa catcggacaa
     8581 caaaaagtgt aatacattgt ttataaagaa attatataac acccctggat taagtcagat
     8641 ttatttcagg cggtccccct caccgccata tttaaagaag agcgcgtacg aaagtacggg
     8701 cttttttttc gtatattgta cacaccgggt gcccccgccc acgtctgtta gggcgagggg
     8761 aaattgtgca catcaactgt ctggttactc agtcaacaat caaaatgcgt aagatagtac
     8821 agaacatata tgcgttatct ggtcacttcc tgtaacttcc tcacttatta aaatgccccc
     8881 aacggaaata catgtcacat gccatcataa aatcgttatg ccagaggaat cgacaatgga
     8941 aaaaatagta aattaagcac ggtacatagc agagtaaaaa ataggcttca aaccaaatcc
     9001 gccattccac aagcgttgcc aataccgaca ccagcgtaat ccacatcatt tcaataaccc
     9061 gcaatatgaa taaccgaagc caaagcggct tggcaaaaaa ggtcgacaac gtattttcac
     9121 tggagaaaaa cagatcgatt tcctgcaaaa aaaacctgcc cagaaaagcg ggccgcttgg
     9181 agaagcgcag aaaaaacaga ttaatcgcca acactacgcc acaaagaatg agcaatgcaa
     9241 ttatcgattt aaacgttaac cactgcccct ctgtcacaaa agaatcactg ttacgcagcg
     9301 ggctgatagt aagcaggtta acaaccgttg agatgcacac cgaaagcagc acatagcgag
     9361 tcagactttt atattttact tttttcgcta ctgaaatagc gacctgcttg acataactgg
     9421 tgcggatcat ccgaaacagc agaaagatga ccaacggccc aagtaacgaa atcaggaaca
     9481 gcgcatataa cgagggtaga agctggtata gcccccaact cattgcactc aacatgatta
     9541 acgacagaaa tcctgccacc agaggttgtg taagaaataa ttctattttg tggttctgct
     9601 ttttcttaaa ccacgaaaaa tcgatattct tcgtgacgca tttataagcc cagtacgcct
     9661 tgagatcaaa aataaaatta tacagcacca gggccagaat cccactaact acaccaatat
     9721 cctctgatgg aaaagtaaac cctgcatttt tccaataaaa gcctatcaga ataagtccaa
     9781 tagcataaat gtatatcaga tttcgtttat cctgaaagcg aataaaagtt aaataatcag
     9841 gaatcatttg aatacctttg catcactaaa tgctttacga caatgcataa ctcgttgctg
     9901 atccacgcca gcaaaatcat ttaacatatt ccccaaatag ggcttattac ttatttcaag
     9961 gaaagctacc ggggttggca tattcagaat atgtatcgag agattcagtc catttcaccg
    10021 tatgagttaa gtgtaatgag agattttctt tgataattga cggagcgacc tcgctttttg
    10081 ctgttacgtt catcagaacc tgatgctctg gcgaagcgat atccagtccg gcaagataat
    10141 cgcgcattgc ctgaacgccg tcttccatca aacgcgtgtg ccaggcaccg ctcacaccga
    10201 gtttaaccgg ttcgtatcca gcggccatca gcagcgtggt aaattcattc aacgcagcct
    10261 gcgttccccc aataacctgc tggcgcggcg tgttatcaca gctaatatcc agcgcaatgc
    10321 ctgattccgt aatcatcgtc tgcagttgtt cgcggttgat gcctttaacc gcctgatctt
    10381 acccagcaat agtggacacg cggctaagtg agtaaactct cagtcagagg tgact
//
LOCUS       DAJTRC010000121        10307 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.121, whole genome shotgun sequence.
ACCESSION   DAJTRC010000121 DAJTRC010000000
VERSION     DAJTRC010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 10307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.121"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            660..743
                     /locus_tag="OO357_003445"
     CDS             660..743
                     /locus_tag="OO357_003445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165328.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Damage inducible protein"
                     /protein_id="HCR5888269.1"
                     /translation="MIDKAIIVLGALIALLELIRFLLQLLN"
     gene            complement(797..2149)
                     /gene="gorA"
                     /locus_tag="OO357_003446"
     CDS             complement(797..2149)
                     /gene="gorA"
                     /locus_tag="OO357_003446"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003861215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HCR5888270.1"
                     /translation="MTKHYDYIAIGGGSGGIASINRAAMYGQKCALIEAKELGGTCVN
                     VGCVPKKVMWHAAQIREAIHMYGPDYGFDTTINKFNWETLIASRTAYIDRIHTSYENV
                     LGKNNVDVIKGFARFVDAKTLEVNGETITADHILIATGGRPSHPDIPGVEYGIDSDGF
                     FALPALPERVAVVGAGYIAVELAGVINGLGAKTHLFVRKHAPLRSFDPMISETLVEVM
                     NAEGPQLHTNAIPKAVVKNADGSLTLELEDGRSETVDCLIWAIGREPANDNINLEAAG
                     VKTNEKGYIVVDKYQNTNIEGIYAVGDNTGAVELTPVAVAAGRRLSERLFNNKPDEHL
                     DYSNIPTVVFSHPPIGTVGLTEPQAREQYGDDQVKVYKSSFTAMYTAVTTHRQPCRMK
                     LVCVGPEEKIVGIHGIGFGMDEMLQGFAVALKMGATKKDFDNTVAIHPTAAEEFVTMR
                     "
     gene            complement(2221..3063)
                     /gene="rlmJ"
                     /locus_tag="OO357_003447"
     CDS             complement(2221..3063)
                     /gene="rlmJ"
                     /locus_tag="OO357_003447"
                     /EC_number="2.1.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase"
                     /protein_id="HCR5888271.1"
                     /translation="MLSYRHSFHAGNHADVLKHTVQSLIIESLKEKDKPFLYLDTHAG
                     AGRYQLGSEHAERTGEYLEGIARIWQQDDLPAELEAYINVVKHFNRSGQLRYYPGSPL
                     IARQLLREQDSLQLTELHPSDYPLLRSGFQKDSRARVEKADGFQQLKAKLPPVSRRGL
                     ILIDPPYEMKTDYQAVVSGIAEGYKRFATGTYALWYPVVLRQQIKRMIHDLEATGIRK
                     ILQIELAVLPDSDRRGMTASGMIVINPPWKLEQQMNNVLSWLHSKLVPAGTGHATVSW
                     IVPE"
     gene            3266..5308
                     /gene="prlC"
                     /locus_tag="OO357_003448"
     CDS             3266..5308
                     /gene="prlC"
                     /locus_tag="OO357_003448"
                     /EC_number="3.4.24.70"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020317218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptidase A"
                     /protein_id="HCR5888272.1"
                     /translation="MTNPLLTPFELPPFSKILPEHVVPAVTKALNDCRENVERVVAQG
                     APYTWENLCQPLAEVDDVLGRIFSPVSHLNSVKNSPELREAYEQTLPLLSEYSTWVGQ
                     HEGLYKAYRDLRDGDHYATLNTAQKKAVDNALRDFELSGIGLPKEKQQRYGEIATRLS
                     ELGNQYSNNVLDATMGWTKLVTDEAGLAGMPESALAAAKAQAEAKELEGYLLTLDIPS
                     YLPVMTYCDNQALREEMYRAYSTRASDQGPNAGKWDNSKVMEEILALRHELAQLLGFE
                     NYAFKSLATKMAENPQQVLDFLTDLAKRARPQGEKELEQLRAFAKAEFGVDELQPWDI
                     AYYSEKQKQHLYSISDEQLRPYFPENKAVNGLFEVVKRIYGITAKERKDVDVWHPDVR
                     FFELYDENNELRGSFYLDLYARENKRGGAWMDGCVGQMRKADGSLQKPVAYLTCNFNR
                     PVNGKPALFTHDEVITLFHEFGHGLHHMLTRIETAGVSGISGVPWDAVELPSQFMENW
                     CWEPEALAFISGHYETGEPLPKELLDKMLAAKNYQAALFILRQLEFGLFDFRLHAEFR
                     PDQGAKILETLAEIKKLVAVVPSPSWGRFPHAFSHIFAGGYAAGYYSYLWADVLAADA
                     FSRFEEEGIFNRETGQSFLDNILSRGGSEEPMDLFKRFRGREPQLDAMLEHYGIKG"
     gene            5316..6068
                     /gene="rsmJ"
                     /locus_tag="OO357_003449"
     CDS             5316..6068
                     /gene="rsmJ"
                     /locus_tag="OO357_003449"
                     /EC_number="2.1.1.242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709277.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(1516)-N(2))-methyltransferase
                     RsmJ"
                     /protein_id="HCR5888273.1"
                     /translation="MKICLIDETGAGDGALSVLAARWGLEHDEDNLMALVLTPEHLEL
                     RKRDEPKLGGIFVDFVGGAMAHRRKFGGGRGEAVAKAVGIKGDYLPDVVDATAGLGRD
                     AFVLASVGCRVRMLERNPVVAALLDDGLARGYADAEIGGWLQERLQLIHASSLTALTD
                     ITPRPQVVYLDPMFPHKQKSALVKKEMRVFQSLVGPDLDADGLLEPARLLATKRVVVK
                     RPDYAPPLANVATPNAVVTKGHRFDIYAGTPV"
     gene            complement(6117..7586)
                     /gene="dtpB"
                     /locus_tag="OO357_003450"
                     /pseudo
     CDS             complement(6117..7586)
                     /gene="dtpB"
                     /locus_tag="OO357_003450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417953.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpB"
     gene            complement(7904..8338)
                     /gene="uspA"
                     /locus_tag="OO357_003451"
     CDS             complement(7904..8338)
                     /gene="uspA"
                     /locus_tag="OO357_003451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspA"
                     /protein_id="HCR5888274.1"
                     /translation="MAYKHILIAVDLSPESKVLVEKAVSMARPYNAKVSLIHVDVNYS
                     DLYTGLIDVNLGDMQKRISEETHHALTELSTNAGYPITETLSGSGDLGQVLVDAIKKY
                     DMDLVVCGHHQDFWSKLMSSARQLINTVHVDMLIVPLRDEEE"
     gene            8732..9067
                     /gene="uspB"
                     /locus_tag="OO357_003452"
     CDS             8732..9067
                     /gene="uspB"
                     /locus_tag="OO357_003452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspB"
                     /protein_id="HCR5888275.1"
                     /translation="MISTVALFWALCVVCIVNMARYFSSLRALLVVLRNCDPLLYQYV
                     DGGGFFTSHGQPNKQVRLVWYIYAQRYRDHHDDEFIRRCERVRRQFILTSALCGLVVV
                     SLIALMIWH"
     gene            complement(9138..>10307)
                     /locus_tag="OO357_003453"
     CDS             complement(9138..>10307)
                     /locus_tag="OO357_003453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HCR5888276.1"
                     /translation="TWYFGLPASSSHTLIGAIIGIGLTNALMTGTSVVDALNIPKVLS
                     IFGSLIVSPIVGLVFAGGLIFLLRRYWSGTKKRARIHLTPAEREKKDGKKKPPFWTRI
                     ALILSAIGVAFSHGANDGQKGIGLVMLVLIGVAPAGFVVNMNATGYEITRTRDAINNV
                     EAYFEQHPALLKQATGADQLVPAPEAGATQPAEFHCHPSNTINALNRLKGMLTTDVES
                     YDKLSLDQRSQMRRIMLCVSDTIDKVVKMPGVSADDQRLLKKLKSDMLSTIEYAPVWI
                     IMAVALALGIGTMIGWRRVATTIGEKIGKKGMTYAQGMSAQMTAAVSIGLASYTGMPV
                     STTHVLSSSVAGTMVVDGGGLQRKTVTSILMAWVFTLPAAVLLSGGLYWFSLQFL"
BASE COUNT         2505 a         2650 c         2773 g         2379 t
ORIGIN      
        1 gcagaggaag gtaataggtg tgaattttga gttggctatt catttgaaag gcggtttgaa
       61 tcaggacgta aaaaaaccga ctttgcgtcg gttttttact cttcagcctg agttggtggc
      121 tctggctgga gtgagaatgc ccacatctaa cggttcaata ctattagcca gtggctaaca
      181 ttgcaagcgt tagcgatttc ttcagctgga gaagggacag tttgctatac atggtggtga
      241 aagaggattt atatactttc atcgggtcgc tgccgtacca agccttgtcc gaatccggac
      301 gtaaaaaaac cgacttcgcg tcggtttttt tactcttcag cccagagttg gtggctctgg
      361 ctggagtgag aatgccatca tctaacgcat caatgctaat accatactga aactattgca
      421 aggttatgct ggttatttag cctaattaca ctgtatgatt atccagttag ctctgaggca
      481 ttttcactct ggcaatgcgc ataaacgctt tcaaagtcct ggtcagaagt acgggtggtg
      541 ccgttaactg atgctctggc cggagtgaga ttgcccacat ctaacaatga ggcatgcgcc
      601 gtgacaggca gtggatgagt aagcggatgc attctcactc catcgcatgg agaaaacggg
      661 tgattgataa agcaatcatc gttcttgggg cgttaattgc gctgctggaa ctgatccgct
      721 ttctgcttca gcttctgaac tgatagcgga aacgtaatta agggctaaga gcacactact
      781 cttagccctt taacatttaa cgcatggtca cgaactcttc tgccgccgtt gggtgaatgg
      841 cgacggtatt gtcgaagtct tttttggttg cgcccatctt cagcgccacc gcgaagccct
      901 gcaacatttc gtccatacca aagccaatgc cgtgaatacc gacaatcttc tcttctggcc
      961 caacgcacac cagcttcatg cggcacggct ggcggtgagt ggtgacggcg gtatacatcg
     1021 cggtgaaaga ggatttatac actttcacct gatcgtcgcc gtactgctcg cgcgcctgcg
     1081 gttccgttaa accaacagta ccaatcggcg gatggctgaa gaccacggtc ggaatgttgc
     1141 tgtaatccag atgctcatcc ggcttgttat taaacaggcg ttcagagaga cggcgacccg
     1201 ctgcaactgc caccggtgtc agttccactg cacccgtgtt atcgcccacc gcgtaaatac
     1261 cttcaatatt ggtgttttga tatttatcga cgacgatata gcctttttcg ttggttttaa
     1321 cgccagcggc ttcgaggttg atgttgtcat tggcaggctc gcgaccaatc gcccaaatca
     1381 ggcaatccac cgtttcactg cgaccatctt ccagctccag cgtcaggcta ccatcggcat
     1441 ttttcactac cgctttcggg atggcgttgg tatgcaactg tgggccttcg gcgttcatca
     1501 cttcgaccag cgtttcagag atcatcgggt cgaagctgcg cagcggcgca tgtttacgca
     1561 caaacagatg cgttttcgcg ccgaggccgt taatcacgcc agccagctca acggcgatgt
     1621 aacccgcgcc aacaaccgcc acgcgctctg gcaaagcagg aagggcgaag aagccatcgg
     1681 aatcaatacc gtattccacg cctggaatat ccgggtggct cggacgaccg ccggtggcga
     1741 tcagaatatg atcggccgtg atggtttcgc cgttaacttc cagcgttttg gcatcaacga
     1801 agcgggcaaa gcctttgatt acatcaacgt tatttttacc gagcacgttt tcataggaag
     1861 tatgaatacg gtcgatatag gcggtacggc tggcgatcaa cgtttcccag ttgaacttgt
     1921 tgatggtggt atcgaaacca taatcagggc cgtacatatg gatcgcttca cggatttgcg
     1981 ccgcgtgcca catcactttt ttcggcacgc agccgacgtt cacgcaggtg ccgcccagct
     2041 ctttggcttc aatcagtgcg catttctggc cgtacatagc cgcgcggttg atggaggcga
     2101 taccgccgct gccgccgccg atggcgatgt aatcatagtg tttagtcatg acatattgtc
     2161 cttatcgttg attaccgcga ttgtagcgcg agacgtaata ggtgccagca atggctgcaa
     2221 ttactccggc acgatccagc ttacggtggc gtgcccggta cctgccggaa ccagtttgct
     2281 gtgcagccac gacagcacgt tattcatctg ctgttccagc ttccacggcg ggttaatcac
     2341 aatcatgccg gaagcggtca tgccacggcg atcgctgtct ggcagcaccg ccagttcaat
     2401 ttgcagaatt ttgcgaatgc cggtcgcttc caggtcgtgg atcatgcgct taatttgctg
     2461 gcgcagcacc accggatacc acaatgcgta agtgccagtg gcgaaacgtt tgtaaccttc
     2521 tgctatcccg ctgaccaccg cctgatagtc ggttttcatt tcatacggcg ggtcgataag
     2581 gattaaacca cggcgagaaa ccggcggcag tttggcctta agttgctgga aaccgtcggc
     2641 tttttcgacg cgtgcacggc tgtctttctg aaatccagaa cgcagcaacg ggtaatcgct
     2701 cgggtgcagt tcggtcagtt gcaggctgtc ttgttcgcgc agtagctggc gagcaatcaa
     2761 cggcgaaccg gggtagtaac gcaactggcc gctacggttg aagtgtttta ccacattgat
     2821 gtacgcctcc agttctgcgg gcaaatcgtc ctgctgccag atacgggcga taccttcgag
     2881 atattcgccg gtacgctcgg catgttcgct acctaactga taacgcccgg cccctgcgtg
     2941 ggtgtcgaga tagagaaacg gtttatcttt ctctttcagc gactcgatga tcaggctctg
     3001 aacggtatgt ttaaggacgt cggcgtggtt gccagcgtga aagctgtggc gataactgag
     3061 catgggtaaa ggtgttccgg taagtaaaaa ttggcccgta ttcggggcgc acaaaagcct
     3121 atcaggacag tataccgaaa agaggccgcc gccgcgaaag cgtaacgttt ctcattgaaa
     3181 ttcactacac ttaaccccat gctacacaca ttatgtaaag cgcctgttga gcgcttcctt
     3241 aacctcttta accaggactg cgctaatgac gaatccgtta ctgactccct ttgaattgcc
     3301 tccgttttct aaaattctcc cggaacatgt cgttccagcc gtgactaagg cattgaacga
     3361 ctgccgcgaa aatgtggagc gcgtagtagc gcaaggggca ccgtacacct gggaaaatct
     3421 ctgccagccg ttggcggaag tggacgatgt tttggggcgt atcttctccc cggtcagcca
     3481 cctgaactcg gtgaaaaata gcccggaact gcgtgaagcg tacgaacaaa ccctgccgct
     3541 gctgtcggaa tacagcacct gggtagggca acatgaaggg ctgtataaag cgtaccgcga
     3601 cctgcgcgat ggcgatcatt acgccacgct gaacacggcg cagaagaaag cggttgataa
     3661 cgccctgcgt gattttgaac tctctggcat aggtctgccg aaagagaaac agcagcgtta
     3721 cggcgaaatt gctacccgtc tttctgaact gggcaaccag tacagcaaca acgtcctcga
     3781 tgcgacgatg ggctggacca aactcgttac cgacgaagcg gggctggcgg ggatgccaga
     3841 aagcgcgctg gctgcggcaa aagcccaggc cgaagcgaaa gagctggaag gctacctgct
     3901 gacgctggat atcccaagct acctgccggt aatgacctac tgcgacaacc aggctctgcg
     3961 cgaagagatg tatcgcgctt acagcacccg cgcctccgat caaggcccga atgccggtaa
     4021 gtgggataac agcaaggtga tggaagagat cctcgcgctg cgtcacgaac tggcgcaact
     4081 gctgggcttt gaaaactacg cctttaaatc ccttgccact aaaatggcag aaaacccgca
     4141 gcaggtgctg gatttcttaa ccgatctggc aaaacgcgcg cgtccgcaag gcgaaaaaga
     4201 gctggagcaa ctgcgtgcct tcgctaaagc cgaatttggt gtcgatgagt tgcagccgtg
     4261 ggatatcgct tactacagcg aaaaacagaa acagcacctc tacagcatca gcgacgagca
     4321 gctgcgtccg tacttcccgg aaaacaaagc ggttaacggc ctgtttgaag tggtgaaacg
     4381 tatttacggc atcaccgcta aagagcgtaa agatgttgat gtctggcatc cggatgtacg
     4441 tttcttcgaa ctgtatgacg aaaataacga actgcgcggc agcttctacc tcgatctgta
     4501 tgcccgtgaa aacaagcgcg gcggggcgtg gatggatggc tgcgtaggcc agatgcgtaa
     4561 agctgatggt tctctgcaaa aaccggtcgc gtatttgact tgtaacttca accgcccggt
     4621 aaatggtaaa ccggcgctgt tcactcacga cgaagtgatc accctgttcc acgagttcgg
     4681 tcacggcctg caccatatgc tgacccgcat cgaaaccgct ggtgtttccg gtatcagcgg
     4741 tgtgccgtgg gatgcggtcg aactgccgag tcagtttatg gaaaactggt gctgggagcc
     4801 ggaggcgctg gcgtttatct ctggtcacta tgaaaccggc gaaccgctgc cgaaagagtt
     4861 gctggataaa atgctggcgg cgaagaacta ccaggcggcg ctgtttattc tgcgtcagct
     4921 ggagttcggc ctgtttgatt tccgccttca tgccgagttc cgcccggatc agggggcaaa
     4981 aatcctcgaa actctggcag aaatcaagaa actggttgcc gtggtgccat ctccgtcctg
     5041 gggccgtttc ccgcacgctt tcagccatat tttcgccggt ggttatgcgg caggttacta
     5101 cagctacctg tgggctgacg tactggcggc agatgctttc tcgcgctttg aggaagaggg
     5161 cattttcaac cgtgaaaccg ggcagtcgtt cctcgacaac attctgagcc gtggcggttc
     5221 agaagagccg atggatctgt tcaaacgctt ccgtggtcgt gaaccgcagc tggatgcgat
     5281 gctggagcat tacggcatta agggctgatc attcagtgaa aatctgctta attgatgaaa
     5341 caggcgccgg agacggcgcc ttatctgttc tggcggcccg ctgggggctg gagcacgatg
     5401 aagacaacct gatggcgctg gtgttaacgc cggaacatct ggaattgcgc aagcgtgatg
     5461 agccaaaact tggcggcatc tttgttgatt ttgttggcgg agcgatggcg caccgacgca
     5521 aattcggcgg tggtcgcggt gaggcggtgg cgaaagcggt gggcattaaa ggcgattatt
     5581 tgccggatgt ggtggacgcc accgcagggc tggggcgcga tgcctttgta ctggcttcag
     5641 tcggctgccg cgtgcggatg ctggagcgta atccagtggt tgccgcgcta ctcgacgacg
     5701 gcctggcgcg tggttatgcg gatgcggaaa tcggcggctg gttgcaggag cggttgcagt
     5761 taattcacgc ctccagcctg acggcgctaa ccgatattac cccacgcccg caggtggtct
     5821 atctcgatcc gatgttcccg cataagcaga aaagcgcgct ggtgaaaaaa gagatgcgtg
     5881 tctttcagtc gctggtgggg ccggatcttg atgccgatgg attactggag cctgcacgct
     5941 tgctggcgac caaacgcgtg gtggtaaagc gcccggacta cgcgccaccg ctggcgaatg
     6001 tcgccacgcc aaacgcggtt gtgactaaag ggcatcgctt tgatatctat gcaggtacgc
     6061 cagtgtaaaa gtaaagcagg gtagcgataa cgctaccctg ctttctgcaa gaataattaa
     6121 tggctttccg gcgtcgcaat catgcgtttc agccacggca ccatcaacag catcactact
     6181 gcaacgccca gcgtgaccag accaatctta ccaaacacgt tggtatagac gggcaacgtc
     6241 tcaagcggat cggtaatgtt gtccggcacc gcggtaaatg ttgccacata gccgcccagc
     6301 aagaacgcgg cagcctgcgt caggaaccac atcccgagaa taaagcccat caaatgctgc
     6361 ggcaccaggg cagcaatcat cgccaggcca agggcgctaa taaacaattc acccaggctc
     6421 tagaataaat acaccagcac gataaaccat ggcgatgtca gcccctggtc atccgcaaac
     6481 cacattcccg cagcagcagc cgtcaaaaag cccagtgagc acataaacat gctgagagta
     6541 aatttcatcg gcatcgagag gtctttgcct ttgttaccca gatgcgtgta aatgcctgcc
     6601 agtattgggc tggcgagtac cacccagaac gggttaagcg cctggaagct gaccgggttg
     6661 atggaaaaac cgagaatttc atgatgcacg ttgttgatgg caaagaagtt cagcgatgtt
     6721 ggcatctggg cgtagagaat gtaaaacacc accgcttcga gcatcaggac aaaggcgaca
     6781 aacattttat tgcgcccggt tttatccagc ttgaatgcct gacgaaagaa gatgatagtg
     6841 acgacgatgg agagaacaat cagcaccaga ttggcgactt ctacgttgtg catcagccat
     6901 gcacatacga agatcatcac cacgctgcca agtaatacgt acaacagttt gctgaagctc
     6961 atcggcttga agtcgggttc agaaccaatg tctttcacca ttccacgaca ggcgatgtaa
     7021 accagtaatg cgataattaa ccccgccccg cacaggttgt aggtgactga ataaccgaat
     7081 ctatcagcga tcacaggggc cagcgataac gctatcaacg agccgatatt gatcgacata
     7141 tagaacaggg tgaatgcgcc atcaagccgc ggatctttcg gcggatagca cttcgaaagc
     7201 aagctggctg ggttagcttt aaacaggccg ttaccgacag cgatagtccc cagggcgatg
     7261 aaaatcaggt caggcttaag tagcgacatg ccggtcatga agtagccaat cgccagcaca
     7321 agtgctccaa gaacaatggt gcgtttggtc cccaacaggt ggtcgccgac atagccgcca
     7381 atggaaatga ggccatagac cagtgcagca aaagcaccaa aagtgacaaa agcctgctct
     7441 tgcgagaatc caagctgttt aacgaagaaa accgccagta cgccctgcac gccgtagtag
     7501 ccgaatcgct cccataactc gacaaaaaag atcatgaaaa atgggcgagg ttgctgcagc
     7561 atccccatgg gtgttgttgt attaatattt attaaccttc caataccatc ctgaaaagta
     7621 tgaacgcgta agggataacg cgcgaaaaag tttacatctg tcagagattc ttaatattga
     7681 tgcggtgaat agattaatac actattacaa cagaaaataa ccagctgatc acactaatga
     7741 tggaaaatat gatggtgtta aattttgtat tggcaggaga ttttgattgc gtacgcagat
     7801 cgtaggccag ataaggggtt tacgctgcat caggtaacaa ggcgcgccaa aaagcaaaac
     7861 gcccgcatat gcgggcgttc aggaacacgt cgtggaggga tgattattct tcttcgtcgc
     7921 gtagcggaac aatcagcata tcaacgtgaa cggtgttgat cagctgacgt gcggaagaca
     7981 tcagtttgct ccagaagtcc tggtggtgac cacaaaccac cagatccata tcgtatttct
     8041 tgattgcatc gaccagaacc tggcctaggt cgccgctgcc gctcagggtt tcagtgattg
     8101 gatagcctgc attagtggaa agctcggtca gcgcatgatg tgtctcttca gagatgcgtt
     8161 tctgcatatc acccagattc acatcaataa gcccggtgta taggtcagag tagtttacat
     8221 ctacgtggat cagagaaact ttcgcattgt aggggcgagc catagagact gctttctcta
     8281 ccagaacttt gctttccggg gagagatcga ctgcgatgag aatgtgttta taagccatag
     8341 tgttactcct tccataaagt tgtcgatgac tggccagcta gcgtttcttg tgccgttacg
     8401 tcacccgcgt cctgcgaact acgtgcgcgg ggctaaccca gccgtaaaga ttcagtgtta
     8461 agaccatcct taccttatag cgacccggat gatccgtcaa tccgccttgc ttacccgtta
     8521 gataaacaaa ttttcgacat gaatattata ttgatagtgg ttaaccttct ggaaaaaaaa
     8581 caacctgatc tcctacacta tctatagagc cgctcggatg ttggtcacca caaaagcggt
     8641 ttctccagga cctggtgtta actgattggc agcgtatcgg gagcggtagc cccggggtgg
     8701 aactccgcgg gcaggtcgcc ggggaggagg tatgataagc accgtcgcat tattttgggc
     8761 tttatgtgtc gtttgcattg ttaacatggc gcgctatttc tcatcactac gcgcgttgtt
     8821 agtggtactg cgtaactgcg atccattgct ctatcaatat gttgatggag ggggcttttt
     8881 tacctcacat ggccaaccca acaaacaggt gcgtctcgtt tggtatatct atgcccaacg
     8941 ttatcgcgat catcacgatg atgagtttat tcgccgctgt gagcgggtgc gtcggcagtt
     9001 tattctgact agcgcattgt gtggtctggt ggtggtcagc ctgattgcat tgatgatttg
     9061 gcattaagca cttcagcaat aataacgtat aaaaaaagcg ggccagtaaa ctgacccgct
     9121 cgttttggtg cgtacgatta caggaactgc aaggagaacc agtacagccc gccggaaagt
     9181 agtaccgcag ccggaagggt aaacacccag gccatcagaa tactggttac ggttttacgc
     9241 tgtaagccgc cgccatctac caccatcgtc cccgcgacag aagaggagag tacgtgagtg
     9301 gtggaaaccg gcatcccggt ataactcgcc aggccgattg acactgccgc cgtcatctgg
     9361 gcagacatcc cctgagcgta ggtcatgcct ttcttaccga ttttctcacc gatagtcgtt
     9421 gccacacgac gccagccaat catcgtaccg atacctaacg ccagcgcgac cgccatgatg
     9481 atccacaccg gcgcatactc gatggtgcta agcatgtcgg acttcagttt cttcaacagg
     9541 cgctgatcgt cagcactcac accaggcatc ttcaccactt tgtcgatagt gtcagaaacg
     9601 cacagcataa tgcggcgcat ctggctacgt tgatcaagcg acagcttgtc gtagctttcc
     9661 acatcggtgg tcaacatacc tttcaggcgg ttgagcgcgt taatggtatt cgacggatgg
     9721 cagtggaact ccgcaggttg cgttgcgcca gcttccggag ccggtactaa ctgatcagca
     9781 ccggtagcct gtttgagcag cgcaggatgc tgctcaaagt aagcttcgac gttgttgatg
     9841 gcatcacggg tacgggtgat ttcgtagcca gtggcattca tgttcaccac gaagcctgct
     9901 ggcgcgacgc caatcaatac caacataacc agaccaatgc ctttctgacc atcgttcgcg
     9961 ccgtgcgaaa acgccacgcc gatagcggaa aggatcagcg cgatacgcgt ccagaacggc
    10021 ggctttttct tgccgtcttt cttttcacgc tccgctgggg tcaggtggat acgggcgcgt
    10081 ttcttggtgc cgctccagta gcgacgcagc aagaaaatca gaccgccagc aaacaccagg
    10141 ccgacaatag gggaaacgat cagagaaccg aaaatactta atactttcgg gatattgagt
    10201 gcatccacca ctgacgtccc ggtcatcaac gcattggtta aaccaatccc gatgatcgcg
    10261 ccaatcagcg tatgagagct ggatgcaggt aaaccaaagt accaggt
//
LOCUS       DAJTRC010000122         9939 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.122, whole genome shotgun sequence.
ACCESSION   DAJTRC010000122 DAJTRC010000000
VERSION     DAJTRC010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9939)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9939)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9939
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.122"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..1025)
                     /gene="bglB"
                     /locus_tag="OO357_003454"
     CDS             complement(<1..1025)
                     /gene="bglB"
                     /locus_tag="OO357_003454"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase BglB"
                     /protein_id="HCR5888277.1"
                     /translation="MKAFPETFLWGGATAANQVEGAWLEDGKGITTSDLQPHGVMGKM
                     EPRILGKENIKDVAIDFYYRYPEDIALFAEMGFTCLRISIAWARIFPQGDEAEPNEAG
                     LAFYDRLFDEMAQAGIKPLVTLSHYEMLYGLVKNYGGWANRAVIDHFEHYARTVFTRY
                     QHKVALWLTFNEINMSLHAPFTGVGLAEESGEAEVYQAIHHQLVASARAVKAYHSLIP
                     EAKIGNMLLGGLVYPLTCQPQDMLQAMEENRRWMFFGDVQARGQYPGYMQRFFRDHNI
                     TIEMTESDAEDLKHTVDFISFSYYMTGCVSHDESINKNAQGNILNMIPNPHLKSSEWG
                     WQIDPVG"
     gene            complement(1044..2921)
                     /gene="bglF"
                     /locus_tag="OO357_003455"
     CDS             complement(1044..2921)
                     /gene="bglF"
                     /locus_tag="OO357_003455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709472.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIABC"
                     /protein_id="HCR5888278.1"
                     /translation="MTELARKIVAGVGGADNIVSLMHCATRLRFKLKDESKAQAEVLK
                     KTPGIIMVVESGGQFQVVIGNHVADVFLAVNSVAGLGEKAQQAPENDEKGNLLNRFVY
                     VISGIFTPLIGLMAATGILKGMLALALTFQWTTEQSGTYLILFSASDALFGFFPIILG
                     YTAGKRFGGNPFTAMVIGGALVHPLILTAFENGQKADAPGLDFLGIPVTLLNYSSSVI
                     PIIFSAWLCSILERRLNAWLPSAIKNFFTPLLCLMVITPVTFLLVGQLSTWISEMIAA
                     GYLWLYQAVPAFAGAVMGGFWQIFVMFGLHRGLVPLCINNFTVLGYDTMIPLLMPAIM
                     AQVGAALGVFLCERDAQKKVVAGSAALTGLFGITEPAVYGVNLPRKYPFVIACISGAL
                     GATIIGYAQTKVYSFGLPSIFTFMQTIPSTGIDFTVWASVIGGVIAIGCAFVGTLMLH
                     FITSKRQPAQGAPQEKTPEVITPPEQGGICSPMTGEIVPLIHVADTTFASGLLGKGIA
                     ILPSVGEVRSPVAGRIASLFATLHAIGIESDDGVEILIHVGIDTVKLDGKFFSAHVNV
                     GDKVNTGDRLISFDIPAIREAGFDLTTPVLISNSDDFTDVLPHGTAQISAGEPLLSII
                     R"
     gene            complement(3055..3876)
                     /gene="bglG"
                     /locus_tag="OO357_003456"
     CDS             complement(3055..3876)
                     /gene="bglG"
                     /locus_tag="OO357_003456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional antiterminator BglG"
                     /protein_id="HCR5888279.1"
                     /translation="MNMQITKILNNNVVVVIDDQQREKVVMGRGIGFQKRAGERINSS
                     GIEKEYALSSHELNGRLSELLSHIPLEVMATCDRLISLAQERLGKLQDSIYISLTDHC
                     QFAIKRFQQNVLLWDIQRLYPKEFQLGEEALTIIDKRLGVQLPKDEVGFIAMHLVSAQ
                     MSGNMEDVAGVTQLMREMLQLIKFQFSLNYQEESLSYQRLVTHLKFLSWRILEHASIN
                     DSDESLQQAVKQNYPQAWQCAERIAIFIGLQYQRKISPAEIMFLAINIERVRKEH"
     gene            complement(4163..4888)
                     /gene="phoU"
                     /locus_tag="OO357_003457"
     CDS             complement(4163..4888)
                     /gene="phoU"
                     /locus_tag="OO357_003457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HCR5888280.1"
                     /translation="MDSLNLNKHISGQFNAELESIRTQVMTMGGMVEQQLSDAITAMH
                     NQDSDLAKRVIEGDKNVNMMEVAIDEACVRIIAKRQPTASDLRLVMVISKTIAELERI
                     GDVADKICRTALEKFSQQHQPLLVSLESLGRHTIQMLHDVLDAFARMDIDEAVRIYRE
                     DKKVDQEYEGIVRQLMTYMMEDSRTIPSVLTALFCARSIERIGDRCQNICEFIFYYVK
                     GQDFRHVGGDELDKLLAGKDSDK"
     gene            complement(4903..5676)
                     /gene="pstB"
                     /locus_tag="OO357_003458"
     CDS             complement(4903..5676)
                     /gene="pstB"
                     /locus_tag="OO357_003458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000063125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HCR5888281.1"
                     /translation="MSMVETAPSKIQVRNLNFYYGKFHALKNINLDIAKNQVTAFIGP
                     SGCGKSTLLRTFNKMFELYPEQRAEGEILLDGDNILTNSQDIALLRAKVGMVFQKPTP
                     FPMSIYDNIAFGVRLFEKLSRADMDERVQWALTKAALWNETKDKLHQSGYSLSGGQQQ
                     RLCIARGIAIRPEVLLLDEPCSALDPISTGRIEELITELKQDYTVVIVTHNMQQAARC
                     SDHTAFMYLGELIEFSNTDDLFTKPAKKQTEDYITGRYG"
     gene            complement(5768..6658)
                     /gene="pstA"
                     /locus_tag="OO357_003459"
     CDS             complement(5768..6658)
                     /gene="pstA"
                     /locus_tag="OO357_003459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HCR5888282.1"
                     /translation="MAMVEMQTTAALAESRRKMQARRRLKNRIALTLSMATMAFGLFW
                     LIWILMSTITRGIDGMSLALFTEMTPPPNTEGGGLANALAGSGLLILWATVFGTPLGI
                     MAGIYLAEYGRKSWLAEVIRFINDILLSAPSIVVGLFVYTIVVAQMEHFSGWAGVIAL
                     ALLQVPIVIRTTENMLKLVPDSLREAAYALGTPKWKMISAITLKASVSGIMTGILLAI
                     ARIAGETAPLLFTALSNQFWSTDMMQPIANLPVTIFKFAMSPFAEWQQLAWAGVLIIT
                     LCVLLLNILARVVFAKNKHG"
     gene            complement(6658..7617)
                     /gene="pstC"
                     /locus_tag="OO357_003460"
     CDS             complement(6658..7617)
                     /gene="pstC"
                     /locus_tag="OO357_003460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008806729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstC"
                     /protein_id="HCR5888283.1"
                     /translation="MAATKPAFNPPGKKGDIIFSVLVKLAALIVLLMLGGIIVSLIIS
                     SWPSIQKFGLAFLWTKEWDAPNDIYGALVPIYGTLVTSFIALLIAVPVSFGIALFLTE
                     LAPGWLKRPLGIAIELLAAIPSIVYGMWGLFIFAPLFAVYFQEPVGNIMSNIPIVGAL
                     FSGPAFGIGILAAGVILAIMIIPYIAAVMRDVFEQTPVMMKESAYGIGCTTWEVIWRI
                     VLPFTKNGVIGGIMLGLGRALGETMAVTFIIGNTYQLDSASLYMPGNSITSALANEFA
                     EAESGLHVAALMELGLILFVITFIVLAASKFMIMRLAKNEGAR"
     gene            complement(7704..8744)
                     /gene="pstS"
                     /locus_tag="OO357_003461"
     CDS             complement(7704..8744)
                     /gene="pstS"
                     /locus_tag="OO357_003461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HCR5888284.1"
                     /translation="MKVMRTTVATVVAATLSMSAFSVFAEASLTGAGATFPAPVYAKW
                     ADTYQKETGNKVNYQGIGSSGGVKQIIANTVDFGASDAPLSDEKLAQEGLFQFPTVIG
                     GVVLAVNIPGLKSGELVLDGKTLGDIYLGKIKKWDDEAIAKLNPGLKLPSQNIAVVRR
                     ADGSGTSFVFTSYLAKVNEEWKNNVGTGSTVKWPIGLGGKGNDGIAAFVQRLPGAIGY
                     VEYAYAKQNNLAYTKLISADGKPVSPTEENFANAAKGADWSKTFAQDLTNQKGEDAWP
                     ITSTTFILIHKDQKKPEQGTEVLKFFDWAYKTGAKQANDLDYASLPDSVVEQVRAAWK
                     TNIKDSSGKPLY"
     gene            complement(8992..>9939)
                     /locus_tag="OO357_003462"
     CDS             complement(8992..>9939)
                     /locus_tag="OO357_003462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001685709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5888285.1"
                     /translation="SGYLITANENKVGTTFNTTVTNGSSYLGRCHCDTGNVGEFPYIY
                     YTSKINQALTYAGVHSNINYYDLNPNLDVGIAIDILGVGYVNAPFEYHANNPSGNTKY
                     NCNRIEPLSISSGAKAIVYFYIKKTFAGKLIIPETKIVTLYGTISRDTPVDYSQPMAD
                     VYIRGDITAPQSCEINNLKPVCFDFKEIPAADFSSVVGSAVTTHKITKTVTIECENLG
                     ILNTDDISTSFYATEPNTDNSMVVTSNSNVGIKIYDKNNKEIKVNGGELPTDMDKSTV
                     YGEKSGSVTFSAAPASLTGARPAPGQFTATATITVEIVR"
BASE COUNT         2565 a         2587 c         2295 g         2492 t
ORIGIN      
        1 aatccaaccg gatcaatttg ccacccccac tctgaacttt tcagatgcgg attggggatc
       61 atattcagta tgttgccctg cgcattttta ttaatgcttt cgtcgtggga aacacaacca
      121 gtcatgtaat aactaaaaga gatgaaatct acagtatgtt ttaaatcttc tgcgtcactt
      181 tcagtcatct caatggtgat attgtggtcg cggaagaaac gctgcatata gccgggatat
      241 tggccacgcg cctgaacatc accaaagaac atccagcgcc ggttctcttc catggcctgc
      301 aacatatcct gtggctggca ggtgaggggg taaaccagcc caccgagaag catattgccg
      361 attttcgctt cggggatcag gctatgatag gctttaactg cccgcgcact ggcaaccagt
      421 tgatgatgga tcgcctgata aacttccgcc tcgccactct cttctgccag ccccacgccc
      481 gtgaatggcg cgtgtaacga catattgatt tcattaaacg tcagccataa cgctacttta
      541 tgttggtagc gggtaaagac cgtgcgggcg taatgctcga agtgatcgat gaccgcccga
      601 ttagcccaac cgccgtagtt tttcaccagc ccatatagca tttcgtaatg ggataacgtt
      661 accagcggct tgatccccgc ctgcgccatt tcatcaaaca gccgatcgta aaacgctaac
      721 cccgcttcat tcggttcggc ttcgtcgccc tgagggaaaa ttcgcgccca ggcaatggaa
      781 atacgcagac aggtgaagcc catctcggca aataacgcga tatcttccgg gtaacggtaa
      841 taaaaatcga tggcgacatc tttgatattc tctttcccca ggatgcgcgg ttccattttt
      901 cccattacgc catgaggctg taaatctgag gtcgtgatcc ctttgccatc ttccagccag
      961 gcaccttcca cctgattggc agctgttgcg ccaccccaaa gaaatgtttc tggaaatgct
     1021 ttcataatta actcctttta tcgttagcga atgatggata acagcggttc acctgcgctt
     1081 atctgcgccg tgccgtgggg taatacgtct gtaaaatcat cgctattact gattaatacc
     1141 ggcgtcgtca gatcaaatcc ggcctcgcga atagcaggga tatcaaaaga aatcagccga
     1201 tcgcctgtat tgaccttgtc acccacgttg acgtgagcgg aaaagaattt gccgtccagt
     1261 tttacggtgt cgataccgac atgaatcagg atctccacac catcatctga ctcaatgcca
     1321 atggcgtgta atgtggcgaa caacgaagca attcgacccg caaccggaga acgcacttca
     1381 ccaaccgagg gcagaatggc gataccttta cccaacaggc cactggcaaa cgtggtatca
     1441 gcgacgtgaa tgagcggcac aatctctccc gtcatcggtg aacagatacc gccctgctca
     1501 ggtggtgtaa taacctctgg tgttttctct tgcggggcac cctgcgctgg ctgacgttta
     1561 ctggtgatga aatgaagcat caacgtacca acaaatgcgc aacctatggc aatgacaccg
     1621 ccaataacgc tggcccagac ggtgaaatca atccccgttg acgggatggt ctgcatgaag
     1681 gtgaaaatac ttggcaaacc aaaggagtaa actttcgttt gcgcgtagcc aatgatggtg
     1741 gcccccaatg ctccactgat acaggcgata acaaaggggt acttacgcgg caggttgacg
     1801 ccatataccg ctggttcggt gataccaaac agacccgtca acgccgctga tcccgccacc
     1861 acttttttct gcgcatcgcg ttcgcagagg aagacgccga gcgccgcccc gacctgcgcc
     1921 ataatggcgg gcattaacag cgggatcatg gtgtcgtagc ccagcacggt gaagttattg
     1981 atacacagcg gcaccaggcc cctgtgcagt ccgaacatga cgaagatttg ccagaagccg
     2041 cccattaccg cgcccgcaaa tgcaggaacc gcctgataaa gccagagata accggcggca
     2101 atcatttcgc ttatccaggt tgatagctgc cccaccagca gaaaggtgac gggtgtgata
     2161 accatcagac atagcaatgg tgtgaagaaa tttttgattg ccgacggtaa ccacgcatta
     2221 agtcggcgtt ccagaatgct gcacaaccag gcagaaaaaa taatgggaat aaccgatgac
     2281 gagtaattca acaatgtgac cggaataccc aggaaatcca gccccggcgc atccgctttt
     2341 tgcccgttct cgaaggcagt cagaattaat ggatgcacta acgctccacc aatcaccatg
     2401 gcagtaaatg gattaccgcc gaagcgtttg cccgcggtgt atcccaggat tatcgggaag
     2461 aacccaaaca aggcatcact ggcgctgaat aaaattaaat aagtaccact ttgttcggtc
     2521 gtccactgaa aagtgagcgc cagagccagc atacctttca ggatcccggt tgccgccatc
     2581 agaccgatca gaggcgtaaa aatacctgaa ataacataaa caaagcggtt tagcagatta
     2641 cctttctcat cattttccgg tgcctgttgc gctttttcgc caaggcctgc cacgctgtta
     2701 accgccagga agacatcggc cacatggtta cctatgacca cctgaaactg accaccgctt
     2761 tccaccacca taataatacc gggggtcttt ttcagtacct cagcttgcgc tttgctttca
     2821 tcctttaact taaaacgtaa tcgcgttgcg caatgcatca gactcacaat gttatctgcg
     2881 cccccgactc ctgcgactat ttttctggct aactccgtca taacttgccc tctaccgctt
     2941 tgcggcaaaa ctccaaaaaa aaacctgaaa aaaacggcct gacgtgaatc aagcaatttt
     3001 tttcaggttt tgcccgctta gtgcggtaac aatcctttac tcagtaataa tatttcagtg
     3061 ttctttgcgc acgcgctcta tatttatggc taaaaacata atctctgcgg gtgaaatttt
     3121 acgttgatac tgcaaaccaa taaaaatggc gatccgttcc gcacattgcc atgcttgcgg
     3181 gtaattttgt tttactgctt gttgtaatga ttcatcacta tcgttaattg aagcatgttc
     3241 aagaatacgc caggataaaa acttcagatg tgtaaccagt cgctgataac tcaagctttc
     3301 ttcctggtaa ttaaggctga actgaaattt tattaattgc agcatttcgc gcattaactg
     3361 cgtgacacct gcaacatcct ccatatttcc gctcatttgg gcactgacca gatgcatggc
     3421 aataaagccc acttcatctt tcggtaactg cacgcccaac cgtttatcaa taatggttaa
     3481 cgcttcttcc cctagctgga actctttcgg gtaaagccgc tggatatccc acagcaacac
     3541 gttttgctga aagcgtttaa tcgcaaactg gcaatggtca gttagcgaga tataaatact
     3601 gtcctgtaat tttcccaagc gctcctgcgc taaagagata agacgatcac aggttgccat
     3661 cacctcaaga ggaatatgac ttaagagttc gcttaatcgc ccgttcagtt catgactgct
     3721 caaggcatac tctttttcta ttccacttga gttaattctt tcgccagcgc gtttttgaaa
     3781 gccaattccg cgccccatga cgactttttc ccgctgttga tcatcaataa ccaccacaac
     3841 attattgttg agaattttgg tgatttgcat gttcatagca aggacctttt ttataaacaa
     3901 aaaaaaaccg acttcaccag tattctctgg ttatgtcagg ttttgcctgc gaatgcagta
     3961 acaatccagt catttattaa tggtttttat aacgaacatc caggttcgga aattaattta
     4021 attgcgtgca gagaaaatag caatgcgcta ttgataaaaa tatgaccatg ctcgcagtta
     4081 ttaaccttgt gtaattttag gaatttataa agttatatat aacaaatccc aataattaag
     4141 ttattgggat ttgtctggtg aattatttgt cgctatcttt tcccgccagc agtttatcca
     4201 gctcatcacc gcctacatga cggaaatcct gacccttcac gtagtagaag ataaactcac
     4261 aaatattctg gcagcggtcg ccgatacgtt cgattgaacg cgcacagaac agcgcggtaa
     4321 gtacgctcgg aatagtgcgc gaatcttcca tcatgtaggt catcagttga cgaacaatac
     4381 cttcgtattc ctgatcgact tttttatctt cacgataaat acgtaccgct tcgtcaatgt
     4441 ccatccgcgc gaacgcgtcc agcacgtcgt gcagcatctg gatggtatga cggcccagcg
     4501 actccagact taccagcaac ggctgatgct gctgggagaa tttctccagc gcagtacggc
     4561 agattttgtc cgccacgtcg ccaatacgct ccagctcggc aatggtttta ctgatcacca
     4621 taaccagtcg caggtcgctc gccgtcggct gacgtttggc gataatgcgc acgcaggctt
     4681 catcgatcgc cacttccatc atgttgacgt tcttgtcgcc ttcgatcacg cgcttcgcca
     4741 gatcgctgtc ctggttatgc atcgcggtga ttgcatcaga aagctgctgc tccaccatgc
     4801 cgcccatggt catcacctgc gtgcggatac tttccagttc ggcgttgaac tggccggaaa
     4861 tatgtttatt aagattgaga ctgtccataa cgcactcctg aatcaaccgt aacgaccggt
     4921 gatgtagtct tctgtttgtt tcttcgctgg cttggtgaac agatcgtccg tgttgctgaa
     4981 ctcaatcaat tcgcccaggt acataaatgc cgtgtggtcg gaacaacgcg cagcctgctg
     5041 catgttgtgg gtgacgatca ccacggtgta atcctgcttc agttcggtga tcagctcttc
     5101 aatacgcccg gtagagatgg ggtcgagcgc cgaacacggt tcgtcgagca gcagcacttc
     5161 cgggcgaatg gcgataccac gcgcaataca cagacgctgt tgctgaccac cagagagaga
     5221 gtaaccgctc tggtgcaatt tatctttggt ttcgttccac aatgcggctt tggtcaatgc
     5281 ccactgcacg cgctcgtcca tgtcggcacg ggagagcttc tcaaacagac gaacgccaaa
     5341 ggcgatgttg tcgtagatgg acatcgggaa cggcgtcggt ttctggaaca ccatgcccac
     5401 tttcgcacgc agcagggcga tatcctgaga gttggtcagg atgttgtcgc catcaagcag
     5461 aatttcacct tccgcacgct gctccgggta cagttcaaac attttgttga aggtacgcag
     5521 cagcgtcgat ttaccgcagc cggacggccc gataaacgcc gtaacctggt ttttggcgat
     5581 atccaggttg atgtttttca gggcatggaa tttgccgtag tagaagttca aattacgaac
     5641 ctgaatttta ctcggggcag tttcaaccat actcattgca atcttttcct catcgtacct
     5701 gatgcttttc gctaatcagg cttaccgtag ggccgggtaa ggcgttcacg ccgcatccgg
     5761 aaaagtgtta accgtgttta ttcttcgcaa aaacaacgcg cgccagaatg ttcagcagca
     5821 ggacgcacag ggtaatgatc aataccccgg cccaggccag ttgttgccat tccgcaaacg
     5881 ggctcatcgc aaacttaaag atcgtcaccg gcaggttggc gatcggctgc atcatgtccg
     5941 tgctccagaa ctggttggag agcgcggtaa acagcagcgg cgcggtttca ccggcaatac
     6001 gggcaatcgc cagcaggata ccggtcataa tcccggacac cgacgctttc agcgtaatcg
     6061 cggagatcat cttccacttc ggtgtaccca gcgcataagc cgcttcacgc aggctgtccg
     6121 gcaccagttt cagcatgttc tcagtggtgc ggataacgat cggcacctgc aacagcgcca
     6181 gcgcaattac gcccgcccag ccggagaagt gctccatctg tgccaccacg atggtgtaga
     6241 caaacagacc aaccacaatc gacggcgcgg agagcagaat gtcgttaatg aagcgaatca
     6301 cttctgccag ccaggattta cgaccatatt ccgccagata aatccccgcc ataatgccca
     6361 gcggcgtacc gaataccgtg gcccacaaaa ttaacagccc gctacccgcc agggcgttcg
     6421 ccagaccacc accttccgta ttgggcggcg gcgtcatttc agtgaacagc gccagcgaca
     6481 taccgtcgat accgcgagtg atggtggaca ttaaaatcca gatcagccag aacaggccga
     6541 aggccatcgt cgccatcgag agcgtcagcg caatacggtt tttgaggcgg cgacgcgcct
     6601 gcattttgcg gcgagattca gccagcgccg cagtggtttg catttcaacc atagccatta
     6661 gcgtgccccc tcattcttag ccaggcgcat aatcataaac ttcgatgcgg cgaggacgat
     6721 gaaggtaatc acaaacagga tcaggcccag ttccatcagt gcggcaacgt gcagaccgga
     6781 ttccgcttcc gcaaattcgt tcgccagcgc agaggtgata ctattgcccg gcatgtacag
     6841 cgaggcgctg tcgagctggt aggtgttacc gatgataaag gtcaccgcca tggtttcacc
     6901 gagcgcgcgg cccagcccca gcatgatgcc gccgataaca ccatttttgg tgaacggaag
     6961 aacgatacgc cagataactt cccaggtggt gcagccaata ccgtaggccg actctttcat
     7021 catcaccggg gtttgttcga acacatcacg cattaccgcc gcaatgtacg gaataatcat
     7081 gatggcgagg atcacgcctg ccgcgaggat accgatacca aatgcggggc cagagaacag
     7141 cgcgccaaca atcgggatat tcgacatgat attgccgacc ggctcctgaa agtaaacggc
     7201 gaacagcggc gcaaagataa acaggcccca catgccgtaa acgatacttg gaatggctgc
     7261 cagcagctca atggcgatac ccagcgggcg tttcagccag ccaggcgcaa gctcagtcag
     7321 gaacagggcg ataccgaaac tcaccgggac ggcgatcagc agcgcgataa acgaagtcac
     7381 caacgtaccg tagatcggca ccagcgcccc gtagatatcg ttcggtgcat cccactcttt
     7441 ggtccatagg aaagccagac caaatttctg aatgctcggc caggaggaga tgatcagaga
     7501 gacaataatg ccacccaaca tcaatagcac aatcagcgcc gccagtttta ccagcacgct
     7561 gaaaattatg tcgccctttt tacccggtgg gttaaaagcg ggcttggttg cagccataag
     7621 ttactcttca gttaaacgcg tttacaaagt cattttgtaa tgccggatgc ggcgtaaaac
     7681 accgtacccg gcctggagtt ttattagtac agcggcttac cgctactgtc tttaatattg
     7741 gtcttccacg cagcgcgaac ctgttcaact acactatccg gcaggctggc gtaatccagg
     7801 tcgttcgcct gtttagcccc ggttttgtac gcccagtcga agaatttcag cacctctgtg
     7861 ccttgttctg gtttcttctg atctttgtgg atcagaatga acgtggtaga ggtaataggc
     7921 catgcatctt cgcctttctg gttggtcaga tcctgagcga aggttttgct ccagtctgca
     7981 ccttttgctg cattagcgaa gttttcttcg gtcggactaa ccggtttacc atcagcggag
     8041 atcagtttgg tgtacgccag gttgttctgc ttcgcgtaag catattcaac ataaccaatt
     8101 gcacccggca gacgctgaac gaacgcggcg ataccgtcgt tacctttacc gcccagaccg
     8161 atcggccatt ttacggtaga gccagtacca acgttgtttt tccactcttc gttcactttc
     8221 gccaggtagc tggtgaagac gaaggaagtc ccggagccat ctgcgcggcg tactacagca
     8281 atgttttgtg aaggcagttt cagacccgga ttcagtttgg cgatggcttc atcatcccac
     8341 ttcttgattt tgcccaggta gatgtcgccg agggttttac catccagcac cagttcgcca
     8401 gacttcagcc ctggaatgtt aaccgccagc accacgccgc caatcacggt cgggaactgg
     8461 aacagacctt cctgagccag tttttcgtca gacagcggcg cgtcagaggc accaaaatca
     8521 acggtattag cgataatctg ttttacgcca ccggaagaac cgataccctg gtagttaact
     8581 ttattaccgg tttctttctg gtaagtgtca gcccatttgg catacaccgg cgcagggaag
     8641 gttgcacctg cacctgtcag gcttgcttct gcaaacacag agaaagcact catcgataag
     8701 gtcgcggcga caacagttgc gacggtggta cgcataactt tcataatgtc tcctgggagg
     8761 attcataaag cattgtttgt tggctacgag aagcaaaata ggacaaacag gtgacagtta
     8821 tatataagga atatgacatt tttatgacag aaaggaatta ccaaataact tactgatttt
     8881 aaatgtgaag atgaaaattc cacatgccat ctttttgtca tgatgcattt aaaagtgata
     8941 aagaaatggg tgagatgaca acgatgtgat gaacaatcgt tgtcagacct attagcgcac
     9001 aatctcaaca gttatcgtcg ctgttgcagt aaattgcccc ggggctgggc gagctccagt
     9061 aaggctcgca ggagcagctg aaaaagttac actacctgat ttttcaccat acactgttga
     9121 tttatccatg tctgttggca actcaccacc gttcactttg atttccttat tatttttgtc
     9181 gtaaattttt atccccacgt tagagtttga agttacgacc attgaattgt ccgtattggg
     9241 ttcggtagca taaaaagacg tactgatatc atcggtattc agtatcccca ggttttcaca
     9301 ctcaatagtg actgttttag taattttatg tgttgttacc gcacttccaa caacagatga
     9361 aaaatctgca gctggtattt ctttaaaatc aaaacaaact ggctttaaat tatttatttc
     9421 gcaactttgc ggagctgtaa tatcgccccg aatataaaca tcagccatcg gctgtgagta
     9481 atccaccggg gtgtcacggc taattgttcc atacaatgtc actatctttg tttcaggaat
     9541 aatcaatttt cctgcaaatg tcttcttgat ataaaaatat actatcgctt tagccccact
     9601 ggatatactt aaaggttcaa tgcggttgca attgtacttt gtattaccag acgggttgtt
     9661 agcatgatat tcaaaaggtg cattcacata cccaacccca agaatatcta tagcaatccc
     9721 aacatcaagg ttcggattaa gatcataata attaatatta gagtgaaccc cagcataagt
     9781 taatgcctgg tttatttttg acgtataata gatatagggg aattctccta cgttaccagt
     9841 atcacaatga cagcgtccaa gatatgagga tccattagtt acagtggtgt taaatgtcgt
     9901 tccaacctta ttttcgttcg cggtaataag atatcccga
//
LOCUS       DAJTRC010000123         9586 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.123, whole genome shotgun sequence.
ACCESSION   DAJTRC010000123 DAJTRC010000000
VERSION     DAJTRC010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9586)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9586)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9586
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.123"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..317)
                     /gene="psiF"
                     /locus_tag="OO357_003463"
     CDS             complement(<1..317)
                     /gene="psiF"
                     /locus_tag="OO357_003463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PsiF"
                     /protein_id="HCR5888286.1"
                     /translation="MKITLLVTLLFGLVFLTTVGAAERTLTPQQQRMTSCNQQATAQA
                     LKGDARKTYMSDCLKNSKSAPGEKSLTPQQQKMRECNNQATQQALKGDDRNKFMSACL
                     KKAA"
     gene            complement(436..1851)
                     /gene="phoA"
                     /locus_tag="OO357_003464"
     CDS             complement(436..1851)
                     /gene="phoA"
                     /locus_tag="OO357_003464"
                     /EC_number="3.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HCR5888287.1"
                     /translation="MKQSTIALALLPLLFTPVTKARTPEMPVLENRAAQGDITAPGGA
                     RRLTGDQTAALRDSLSDKPAKNIILLIGDGMGDSEITAARNYAEGAGGFFKGIDALPL
                     TGQYTHYALNKKTGKPDYVTDSAASATAWSTDVKTYNGALGVDIHEKDHPTILEMAKA
                     AGLATGNVSTAELQDATPAALVAHVTSRKCYGPSATSEKCPGNALEKGGKGSITEQLL
                     NARADVTLGGGAKTFAETATAGEWQGKTLREQAQARGYQLVNDAASLNSVTEANQQKP
                     LLGLFADGNMPVRWLGPKATYHGNIDKPAVTYTPNPQRNDSVPTLAQMTDKAIELLSK
                     NEKGFFLQVEGASIDKQDHAANPCGQIGETVDLDEAVQRALEFAKKEGNTLVIVTADH
                     AHASQVVAPDTKAPGLTQALNTKDGAVMVMSYGNSEEDSQEHTGSQLRIAAYGPHAAN
                     VVGLTDQTDLFYTMKAALGLK"
     gene            complement(1952..2212)
                     /gene="iraP"
                     /locus_tag="OO357_003465"
     CDS             complement(1952..2212)
                     /gene="iraP"
                     /locus_tag="OO357_003465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraP"
                     /protein_id="HCR5888288.1"
                     /translation="MKNLIAELLFKLAQKEEESKELCAQVEALEIIVTAMLRNMAQND
                     QQRLIDQVEGALYEVKPDASIPDDDTELLRDYVKKLLKHPCQ"
     gene            complement(2394..2597)
                     /locus_tag="OO357_003466"
     CDS             complement(2394..2597)
                     /locus_tag="OO357_003466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888289.1"
                     /translation="MLQSSADKACNVLVKKVRYLKDVRKITKMMNGKLIYSWLKWQCG
                     EFFSLNYKLTKRIYFIAFIYNKG"
     gene            2675..3769
                     /gene="ddlA"
                     /locus_tag="OO357_003467"
     CDS             2675..3769
                     /gene="ddlA"
                     /locus_tag="OO357_003467"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HCR5888290.1"
                     /translation="MEKLRVGIVFGGKSAEHEVSLQSAKNIVDAIDKSRFDVVLLGID
                     KQGQWHVSDASNYLLNADDPAHIALRPSATSLAQVPGKHEHQLIDAQNGQPLPTVDVI
                     FPIVHGTLGEDGSLQGMLRVANLPFVGSDVLASAACMDKDVTKRLLRDAGLNIAPFIT
                     LTRANRHNISFAEVESKLGLPLFVKPANQGSSVGVSKVTSEEQYAIAVDLAFEFDHKV
                     IVEQGIKGREIECAVLGNDNPQASTCGEIVLTSDFYAYDTKYIDEDGAKVVVPAAIAP
                     EINDKIRAIAVQAYQTLGCAGMARVDVFLTPENEVVINEINTLPGFTNISMYPKLWQA
                     SGLGYTDLITRLIELALERHAADNALKTTM"
     gene            complement(3793..4005)
                     /locus_tag="OO357_003468"
     CDS             complement(3793..4005)
                     /locus_tag="OO357_003468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414914.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2754 domain-containing protein"
                     /protein_id="HCR5888291.1"
                     /translation="MNLPVKIRRDWHYYAFAIGLIFILNGVVGVLGFEAKGWQTYAVG
                     LVTWVISFWLAGLIIRRRDEETENAQ"
     gene            4265..4573
                     /locus_tag="OO357_003469"
     CDS             4265..4573
                     /locus_tag="OO357_003469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiY family protein"
                     /protein_id="HCR5888292.1"
                     /translation="MADFTLSKSLFSGKYRNASSTPGNIAYALFVLFCFWAGAQLLNL
                     LVHAPGVYERLMQVQETGRPRVEIGLGVGTIFGLIPFLAGCLIFAVVALWLHWRHRRQ
                     "
     gene            complement(4632..5726)
                     /gene="yaiW"
                     /locus_tag="OO357_003470"
     CDS             complement(4632..5726)
                     /gene="yaiW"
                     /locus_tag="OO357_003470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="surface-exposed outer membrane lipoprotein YaiW"
                     /protein_id="HCR5888293.1"
                     /translation="MSRVNHLSSLSLLAVLVLAGCSSQAPQPLKKGEKAIDVASVVRQ
                     KMPASVKDRDAWAKDLATTFESQGLAPTLENVYSVLAVAQQESNYQADPAVPGLSKIA
                     WQEIDRRAERMHIPAFLVHTALKIKSPNGKSYSERLDSVRTEKQLSAIFDDLISMVPM
                     GQTLFGSLNPVRTGGPMQVSIAFAEQHTKGYPWKMDGTVRQEVFSRRGGLWFGTYHLL
                     NYPASYSAPIYRFADFNAGWYASRNAAFQNAVSKASGVKLALDGDLIRYDSKEPGKTE
                     LATRKLAGKLGMSDSEIRRQLEKGDSFSFEETALYKKVYQLAEAKTGKSLPREMLPGI
                     QLESPKITRNLTTAWFAKRVDERRARCMKQ"
     gene            complement(5739..6959)
                     /gene="sbmA"
                     /locus_tag="OO357_003471"
     CDS             complement(5739..6959)
                     /gene="sbmA"
                     /locus_tag="OO357_003471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706191.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide antibiotic transporter SbmA"
                     /protein_id="HCR5888294.1"
                     /translation="MFKSFFPKPGTFFLSAFVWALIAVIFWQAGGGDWVARITGASGQ
                     IPISAARFWSLDFLIFYAYYIVCVGLFAFFWFIYSPHRWQYWSILGTALIIFVTWFLV
                     EVGVAVNAWYAPFYDLIQTALSSPHKVTIEQFYREVGVFLGIALIAVVISVLNNFFVS
                     HYVFRWRTAMNEYYMANWQQLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIA
                     FLPVLVTLSAHVPELPIIGHIPYGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVE
                     AAYRKELVYGEDDATRATPPTVRELFSAVRKNYFRLYFHYMYFNIARILYLQVDNVFG
                     LFLLFPSIVAGTITLGLMTQITNVFGQVRGAFQYLINSWTTLVELMSIYKRLRSFEHE
                     LDGDKIQEVTHTLS"
     gene            7311..8468
                     /gene="ampH"
                     /locus_tag="OO357_003472"
     CDS             7311..8468
                     /gene="ampH"
                     /locus_tag="OO357_003472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine-
                     carboxypeptidase/endopeptidase AmpH"
                     /protein_id="HCR5888295.1"
                     /translation="MKRSLLFSAVLCAASLTSVHAAQPITEPEFASDIVDRYADHIFY
                     GSGATGMALVVIDGNQRVFRSYGETRPGNNVRPQLDSVVRIASLTKLMTSEMLVKLLD
                     QGTVKLNDPLSKYAPPGARVPTYNGTPITLVNLATHTSALPREQPGGAAHRPVFVWPT
                     REQRWKYLSTAKLKAAPGSQAAYSNLAFDLLADALANASGKPYTQLFEEQITHPLGMK
                     DTTYTPSPDQCRRLMVAERGASPCNNTLAAIGSGGVYSTPGDMMRWMQQYLSSDFYQR
                     SNQADRMQTLIYQRAQFTKVIGMDVPGKADALGLGWVYMAPKEGRPRIIQKTGGGGGF
                     ITYMAMIPQKNIGAFVVVTRSPLTRFKNMSDGINDLVTELSGNKPLVIPAS"
     gene            complement(8469..8972)
                     /locus_tag="OO357_003473"
     CDS             complement(8469..8972)
                     /locus_tag="OO357_003473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414909.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5888296.1"
                     /translation="MLSVVKPLQEFGKLDKCLSRYGTRFEFNNEKQVIFSSDVNNEDT
                     FVILEGVISLRREENVLIGITQAPYIMGLADGLMKNDIPYKLISEGNCTGYHLPAKQT
                     RATLLSMIDWNEELRSRIGVMNYIHQRTRISRSVVAEVLAALRKGGYIEMNKGKLVAI
                     NRLPSEY"
     gene            complement(9060..>9586)
                     /locus_tag="OO357_003474"
     CDS             complement(9060..>9586)
                     /locus_tag="OO357_003474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5888297.1"
                     /translation="LSYSHFNNDLSATMSNGTYVDGSTNSDAWGFGLKTGYDFKLGDA
                     GYVTPYGSISGLFQSGDDYQLSNDMKVDGQPYDSMRYELGVDAGYTFTYSEDQALTPY
                     FKLAYVYDDSNNDNDVNGDSIDNGTEGSAVRVGLGTQFSFTKNFSAYTDANYLGGGDV
                     DQDWSANVGVKYTW"
BASE COUNT         2378 a         2447 c         2282 g         2479 t
ORIGIN      
        1 gcggctttct tgaggcaggc actcataaac ttattacgat catcaccttt cagagcttgt
       61 tgcgttgctt gattattaca ttcacgcatc ttttgctgct gtggcgtcaa acttttttca
      121 ccaggcgcag acttgctgtt cttcaggcaa tcactcatgt aggtcttacg agcatcccct
      181 ttcaacgcct gcgccgtcgc ctgctgatta caggaggtca tacgctgttg ttgtggggtt
      241 aaggttctct cggcagcgcc gacggtggtt aaaaaaacca gaccaaaaag caaggtaacc
      301 agtaatgtta ttttcatagc accatccctc ttcatgtttt aaccatgagc gtttgcgccc
      361 gtgatctgcc attaagtctg gttgctaaca gcaaaaaaac cacccggcag cgaaaattca
      421 ctgccgggcg cagttttatt tcagccccag agcggctttc atggtgtaga agagatcggt
      481 ctggtcggtc agtccaacaa cattggcggc atgcgggcca tacgccgcaa tacgcaactg
      541 actgccggta tgttcttgtg aatcctcttc ggaattcccg taactcatca ccatcactgc
      601 gccatcttta gtatttagcg cctgagtgag gcccggcgct ttggtatccg gcgcaacaac
      661 ctggctggcg tgggcgtgat cagcggtgac tatgaccagc gtgttaccct cctttttagc
      721 gaattccagc gcccgttgta cggcttcatc gagatcgacc gtctcgccaa tttgcccaca
      781 agggttcgca gcgtgatcct gtttatcgat tgacgcacct tcaacttgca ggaaaaatcc
      841 tttctcattt ttactcaaca attcaatggc tttgtcggtc atctgtgcca gggttggtac
      901 actgtcatta cgttgcggat taggcgtata ggtgactgcg ggcttatcga tattgccgtg
      961 gtacgttgct ttcggcccta gccagcgcac tggcatattg ccgtcagcaa acaggccaag
     1021 caggggtttt tgctgattcg cttccgtcac cgaattcagt gaggcagcat cgttcaccaa
     1081 ctgataacca cgcgcctgtg cctgttcacg cagcgttttt ccctgccatt caccagcggt
     1141 tgccgtttca gcaaaggttt ttgcgccgcc gccaagcgta acgtcggcac gagcgttaag
     1201 cagctgttcg gtaatcgatc cttttccgcc tttttccaga gcgttacccg gacatttttc
     1261 actggtcgcg ctcggaccgt agcatttgcg cgaagtcaca tgtgccacca gcgcagcggg
     1321 cgtggcatcc tgcaactctg cggtagaaac gttaccggtc gccagacctg cggcttttgc
     1381 catttccaga atcgttgggt gatctttttc gtgaatatcg acgcccagcg cgccgttata
     1441 ggttttgaca tcggttgacc aggcggttgc tgatgcagcc gagtcggtga cgtagtccgg
     1501 tttgccagtt tttttattca gcgcatagtg agtgtattgc ccggtaagcg gtaaggcatc
     1561 gataccttta aaaaagccgc ccgcaccttc ggcataatta cgtgcggcag taatttccga
     1621 atcccccatc ccatcgccaa tcagcaaaat aatatttttt gcaggtttat cgctaagaga
     1681 atcacgcaga gcggcggtct gatcacccgt taagcggcga gcaccgccgg gtgcagtaat
     1741 atcgccctga gcagcccggt tttccagaac aggcatttct ggtgtccggg cttttgtcac
     1801 aggggtaaac agtaacggta agagtgccag tgcaatagtg ctttgtttca ctttattttc
     1861 tccatgtaca aatacattaa aaaataaaaa caaagcgact ataagtctcg gccgtgacaa
     1921 ctttatgaca gctgttgaaa agattaactt tttactgaca aggatgcttc aataacttct
     1981 ttacgtaatc gcgcagcagc tctgtatcgt cgtcaggaat gctggcatcg ggctttacct
     2041 cgtacagcgc cccctctacc tgatcaatca accgctgttg gtcattttgc gccatattgc
     2101 gaagcattgc agtgacgata atctccaaag cttctacctg cgcacacagt tctttcgact
     2161 cttcttcttt ttgggcaagc ttaaataaca actcagcaat gagatttttc atgtctgtat
     2221 ttccttatcc aaagtatgga gaagttaaca ttatgtttca caacttcata gtgtctggaa
     2281 gtattatttt tatgacaaag gaaatacttt gcgcaaatat tttttgtttg attgccagcg
     2341 aaacgtttct cttatttaaa taattaccca cacaaaatat aggcttttaa ttatcaacct
     2401 ttattataaa taaaagctat gaaatatatt ctcttcgtta acttataatt aagagaaaaa
     2461 aactcaccgc attgccattt aagccaggaa taaattagct tcccgttcat catttttgtg
     2521 atcttgcgca catctttgag ataacgcact ttcttcacca gtacgttgca cgctttgtca
     2581 gcagaagatt gcaacaatct ttcagaaggc cgcgaatccc ccgttttgtc gtggtaatgt
     2641 atgcactttg tgttagacgg gataggtttt taagatggaa aaactgcggg taggaatcgt
     2701 ttttggtggt aaatcagcgg aacatgaagt gtctctgcaa tcggcaaaaa acattgtcga
     2761 tgccattgat aaaagtcgct tcgacgttgt gctgctgggc attgataaac aagggcaatg
     2821 gcacgtcagc gatgccagca attatctgct aaatgcagac gatcctgccc atattgcgtt
     2881 gcgcccttcg gcgaccagcc ttgcgcaggt gccaggtaaa catgagcatc agcttatcga
     2941 cgcgcaaaac ggtcagccgt tgccgacggt ggatgtcatt ttcccgattg tccacggtac
     3001 gctgggcgaa gatggttcct tgcagggaat gctgcgggtc gccaatttac cgtttgtagg
     3061 ttctgatgtt ctggcttcag cagcctgtat ggataaagat gtcaccaaac gtctgctacg
     3121 cgatgccggg ctgaacattg cgccatttat taccctgacg cgcgctaatc gtcacaacat
     3181 cagttttgcc gaagtggagt ctaaactggg gttaccactg tttgtaaaac cggctaatca
     3241 gggctcttct gttggtgtca gcaaagtaac cagtgaagaa cagtacgcaa ttgccgtcga
     3301 tctggcgttc gagttcgatc ataaagtgat cgttgagcaa gggatcaaag gtcgtgagat
     3361 cgaatgcgca gttctgggca acgacaatcc gcaagccagc acctgtggcg agatcgtact
     3421 caccagcgat ttctatgcct acgacaccaa gtacattgac gaagatggcg cgaaagtggt
     3481 agttccggca gccattgcac cagagatcaa cgataagatc cgggcgattg ccgttcaggc
     3541 ttatcagacg ttgggatgcg caggcatggc gcgtgtagac gtgtttttaa ccccagagaa
     3601 cgaagtggtg atcaacgaga tcaacacact gcctggcttc accaacatca gtatgtatcc
     3661 gaagctgtgg caagccagcg gtctgggtta caccgatctg atcacacgtt tgattgagct
     3721 ggcgctggag cgtcacgctg cggataacgc attgaaaacc acaatgtaat attttaacgc
     3781 ccgataatat tcttattggg cgttttcagt ttcttcatcg cgacgacgaa taatcaaccc
     3841 cgccagccag aaactaatca cccacgtcac cagaccgacg gcataggtct gccaaccttt
     3901 tgcttcaaat cccagtaccc ccaccacgcc attaagaatg aatataaggc caatggcgaa
     3961 cgcatagtag tgccagtcac ggcggatttt tacaggcaga ttcatggcag ctccgttaac
     4021 gaaaaacaac atccttgcat attctgtaac aaccgtctgt ggtgtagaca acattttcgt
     4081 aattattaat aaatttaact taaggttgcc attttgtgac tcacaacgaa gattcacgct
     4141 tcgggaatat tcccaacatc aatttaccat gattctgata ttgacaatct aaatgacctg
     4201 tcagacttgc cataagccgc tggtatcaca ccagaagaga agcagatgac ttccggaggt
     4261 ttttatggct gatttcaccc tgtcaaaatc gctgtttagc ggaaaatatc gcaatgcctc
     4321 ttcaacgcct ggcaacattg cctatgcgtt gtttgtgctg ttttgctttt gggctggggc
     4381 gcaattgctg aacctgttag tgcatgcgcc cggcgtctat gagcgtttaa tgcaggtcca
     4441 ggaaacaggt cgcccacggg tggaaattgg tttaggtgtc ggcaccattt tcgggctgat
     4501 cccgttttta gcaggctgcc tcatttttgc agtggtggcg ctatggctgc actggcgaca
     4561 tcgccgccag taaaatttac gttgccagat gcggcctgaa acgccttatc tggcctgaca
     4621 ttcctgtttg atcactgttt catacaacgc gcccgccgtt cgtctacgcg cttcgcaaac
     4681 caggccgtag tcaggttgcg ggtgattttc gggctttcca gttgaatgcc aggcaacatt
     4741 tcgcggggta gtgatttacc cgttttcgct tcggcaagtt gataaacttt cttgtacagc
     4801 gccgtctcct caaaagagaa gctgtcacct ttctctaact gacggcgaat ttcgctgtcg
     4861 ctcatcccca gttttcccgc cagtttgcgc gttgccagtt cggttttccc gggttcttta
     4921 ctgtcgtaac gaattaaatc accgtccagt gccagcttca cgccgctggc cttactgact
     4981 gcgttctgaa acgcggcatt acggctggcg taccagccag cgttaaaatc agcaaagcgg
     5041 tatatcggtg cgctatagct ggcgggataa ttcagtaaat ggtaagtacc aaaccacaac
     5101 ccgccgcggc gactgaagac ttcctgacga actgtaccgt ccattttcca cggataccct
     5161 ttggtatgct gttcggcaaa agcaatgctg acctgcatcg gcccaccggt gcgcaccgga
     5221 ttaagcgaac caaacagcgt ctgccccatt ggtaccatgc tgatcagatc gtcaaaaatc
     5281 gcgctcaatt gcttttctgt acgcaccgaa tctaaccgtt cgctataact tttgccgttt
     5341 ggcgatttga tttttagcgc ggtatgaacc agaaaagcag gaatgtgcat ccgttcagca
     5401 cgacggtcaa tctcttgcca ggcgatcttg cttaagcccg gaacggccgg atctgcctga
     5461 taatttgact cctgctgcgc caccgccagc accgagtaga cgttttccag cgtcggtgcc
     5521 agcccctgac tttcaaaggt ggtggcgaga tctttcgccc aggcatcgcg gtctttcacg
     5581 ctcgcaggca tcttctgccg cacgacgctc gctacatcta tcgctttttc gccctttttc
     5641 aacggttgcg gagcctgact actacacccc gctaaaacca gaaccgccaa caatgacagc
     5701 gaagatagat gattgacgcg cgacattact tctccttttt agctcaaggt atgggttact
     5761 tcctgaattt tgtcaccatc cagctcatgt tcaaagctgc gcagacgttt gtagatagac
     5821 atcaactcaa ccagtgtggt ccatgagtta atcaggtact ggaaagcacc gcgaacctga
     5881 ccaaaaacgt tggtaatctg cgtcatcagg ccgagcgtaa tcgtaccggc aacaattgac
     5941 ggaaacagca agaacaaacc gaaaacgtta tcgacctgca aatagagaat gcgggcgatg
     6001 ttgaaataca tatagtgaaa atagaggcga aaatagtttt tccgtacggc gctaaacagc
     6061 tcgcgtaccg taggcggcgt cgcgcgcgtg gcatcgtctt caccataaac cagctcttta
     6121 cggtaggcgg cctctactcg ctggttttta aactccagcc ccggcagttt gatccctacc
     6181 actgccagca atccggtccc catcagcgac cagacgatag cggcaatcac cagaccatac
     6241 ggaatgtgcc cgataatcgg cagctccggc acatgcgcgg agagcgttac cagcaccggc
     6301 aggaaggcga tcaacgtcat gatggcgttg ataaaactga cgcccatatt ctccagcgtt
     6361 gaagcaaaac gcatggtgtc ttcctgcaca cgctgtgcgg caccttcgat atgacgcagt
     6421 tgttgccagt tcgccatgta atattcgttc atcgccgtac gccagcggaa cacgtagtga
     6481 ctgacaaaga agttgttcag tacactgatc accaccgcga tcagcgcaat ccccagaaag
     6541 acgcccactt cgcggtaaaa ttgttcgata gtgactttat gcggcgaact tagcgcggtt
     6601 tgaatcagat catagaacgg cgcataccag gcgttgacgg cgaccccgac ttccaccaaa
     6661 aaccaagtga cgaagataat cagcgcagta ccgagtattg accagtattg ccaacggtgc
     6721 gggctgtaga taaaccagaa aaatgcaaaa agtcctacgc aaacaatgta gtaagcgtaa
     6781 aaaatcagga aatccaacga ccagaaacgc gcggcgctaa tcgggatctg cccggaagcg
     6841 ccggtgatac gtgccaccca gtcaccccca ccggcttgcc agaagataac ggcaatcaat
     6901 gcccaaacaa aggccgagag aaaaaacgtt cccggctttg ggaaaaaaga cttaaacata
     6961 tgcactcctg ctaacttctt atcgttttaa caactgctgt gtattaaccg catgaccgct
     7021 acaaagcgtc agccaaatga aaccgcccat tgtgacccat agcaaggcgc ttagttcgcc
     7081 agcctgacca taagaattgt ttcgacggat ttcacaaacc aaagattgtt tacataagcg
     7141 gattaacacg cgcaggatca caaatgcagg cattggtgag caggaaaaag atcgtgttta
     7201 cgcgccgtta cgtttttaac actattattg cactaattcg ccctttgcaa tccatcaatg
     7261 agtagtataa atacgctcag ttaccttcat tcaatctatg gacaccaccg ttgaaacgta
     7321 gtctgctttt ttctgccgtg ctgtgtgcgg cgtcattgac ctccgtccat gcggcgcagc
     7381 cgatcactga accggagttt gcctctgata ttgtcgatcg ttatgccgat catatttttt
     7441 acggcagcgg tgccacgggg atggcgctgg ttgttatcga cggtaaccag cgcgtctttc
     7501 gtagttatgg tgaaacgcga cctggtaata acgttcgccc gcagctggat tcggtcgtgc
     7561 gtattgcttc cctcaccaaa ctaatgacca gtgaaatgct ggtgaaattg ctcgaccagg
     7621 ggaccgtaaa gttaaacgat ccgctaagta aatatgctcc gccaggcgca cgcgtgccga
     7681 cctacaatgg gacgcccatt accctagtga atctggcaac ccataccagc gccctgcccc
     7741 gtgaacagcc cggtggcgcg gcacatcgtc cggtgtttgt ctggccaacg cgcgagcaac
     7801 gctggaaata cctttctacg gcgaagctga aagccgcgcc aggcagtcag gcggcgtact
     7861 ctaaccttgc gttcgacctg ctggcagatg cactggcgaa tgcgtccggc aagccttata
     7921 cccagttgtt tgaagagcag attacccatc cattggggat gaaagacacc acctacaccc
     7981 cctcaccgga tcagtgccgc cgtttaatgg ttgctgagcg cggtgccagt ccgtgcaata
     8041 acacgctggc ggcaattggc agcggcggcg tctattccac gcctggcgat atgatgcgct
     8101 ggatgcagca gtatttgtca tctgattttt atcagcgcag taaccaggca gatcgcatgc
     8161 agacgttgat ttatcagcgc gcgcagttta cgaaagtgat tggcatggat gtccccggca
     8221 aagcggatgc gcttggttta ggctgggtat atatggcccc gaaagagggt cgtccgagga
     8281 ttattcagaa gacaggcggt ggtggcggtt tcattaccta tatggcgatg atcccgcaga
     8341 agaatattgg cgcgtttgtg gtggtcactc gttcgccgtt gacgcgcttt aaaaatatga
     8401 gcgatggcat caatgatctg gtgaccgagc taagcgggaa taaaccgttg gttatccccg
     8461 catcctgatt aatactctga aggcaaacgg ttgatagcga ccagtttgcc tttattcatt
     8521 tcgatatagc cgcctttacg caaagcagcg agaacttctg cgacgacaga acgcgatatg
     8581 cgtgtacgtt gatggatata attcatcaca ccaatacgtg atcgcaattc ttcattccag
     8641 tcaatcattg ataataatgt tgcgcgggtt tgtttggctg gtagatgata tcccgtacaa
     8701 tttccttctg atattaattt gtatggtata tcgtttttca ttaaaccatc agccagcccc
     8761 ataatataag gagcctgggt aataccgata agtacgtttt cttctctacg cagagagata
     8821 actccttcta aaataacgaa agtatcttcg ttattgacat cactggaaaa tataacttgc
     8881 ttttcattat taaactcgaa gcgcgtaccg tatctggaca aacatttatc gagcttacca
     8941 aattcctgaa gaggtttaac tacagataac atttgcgcgt cctttgcagt aatgcccgtc
     9001 aaatccttga cgggcattat ttagattaaa ttaccagtat ttcttcggag tgaagaatat
     9061 taccaagtat atttaacacc cacgttcgcg gaccagtctt gatctacgtc accaccaccg
     9121 aggtagttag catcggtata ggcgctgaag ttcttggtga agctgaactg agtacccaga
     9181 ccaacacgta ccgcagaccc ttcagtaccg ttatcgatgg aatcaccgtt cacatcgtta
     9241 tcgttgttag agtcgtcgta gacgtaagcc agtttgaagt acggagtcag agcctgatct
     9301 tcgctgtagg tgaaggtata acctgcatct acacccagtt cataacgcat gctgtcgtaa
     9361 ggctgaccgt caactttcat gtcgttgctc agctggtagt catcaccaga ctggaacaga
     9421 ccagaaatgc tgccgtaagg agtcacgtaa ccagcatcac ccagtttgaa gtcgtaaccg
     9481 gttttcaaac cgaagcccca agcgtcggag ttggtgctac cgtcaacgta agtaccgttg
     9541 ctcatggttg cagacaggtc gttgttgaag tgagagtagc tcaagc
//
LOCUS       DAJTRC010000124         9550 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.124, whole genome shotgun sequence.
ACCESSION   DAJTRC010000124 DAJTRC010000000
VERSION     DAJTRC010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9550)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9550)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9550
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.124"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            123..254
                     /locus_tag="OO357_003475"
     CDS             123..254
                     /locus_tag="OO357_003475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001344347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA invertase"
                     /protein_id="HCR5888298.1"
                     /translation="MLIGYVRVSTNDQNTDLQRNAFICAGSELIVARMGSWPEVVMA"
     gene            514..1509
                     /gene="pgl"
                     /locus_tag="OO357_003476"
     CDS             514..1509
                     /gene="pgl"
                     /locus_tag="OO357_003476"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HCR5888299.1"
                     /translation="MKQTVYIASPESQQIHVWNLNHEGALTLTQVVDVPGQVQPMVVS
                     PDKRYLYVGVRPEFRVLAYRIAPDDGALTFAAESVLPGSPTHISTDHQGQFVFVGSYN
                     AGNVSVTRLEDGLPVGVVDVVEGLDGCHSANISPDNRTLWVPALKQDRICLFTVSDDG
                     HLVAQDPAEVTTVEGAGPRHMVFHPNEQYAYCVNELNSSVDVWELKDPHGNIECVQTL
                     DMMPENFSDTRWAADIHITPDGRHLYACDRTASLITVFSVSEDGSVLSKEGFQPTETQ
                     PRGFNVDYSGKYLIAAGQKSHHISVYEIVGEQGLLHEKGRYAVGQGPMWVVVNAH"
     gene            complement(1550..2503)
                     /locus_tag="OO357_003477"
                     /pseudo
     CDS             complement(1550..2503)
                     /locus_tag="OO357_003477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001473949.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
     gene            2687..3739
                     /locus_tag="OO357_003478"
     CDS             2687..3739
                     /locus_tag="OO357_003478"
                     /EC_number="5.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalomesaconate tautomerase"
                     /protein_id="HCR5888300.1"
                     /translation="MKKIPCVMMRGGTSRGAFLLAEHLPEDQTQRDKILMAIMGSGND
                     LEIDGIGGGNPLTSKVAIISRSSDPRADVDYLFAQVIVHEQRVDTTPNCGNMLSGVGA
                     FAIENGLIAATSPVTRVRIRNVNTGTFIEADVQTPNGVVEYEGSARIDGVPGTAAPVA
                     LTFLNAAGTKTGKVFPTDNQIDYFDDVPVTCIDMAMPVVIIPAEYLGKTGYELPAELD
                     ADKALLARIESIRLQAGKAMGLGDVSNMVIPKPVLISPAQKGGAINVRYFMPHSCHRA
                     LAITGAIAISSSCALEGTVTRQIVPSVGYGNINIEHPSGALDVHLSNEGQDATTLRAS
                     VIRTTRKIFSGEVYLP"
     gene            3815..5248
                     /locus_tag="OO357_003479"
     CDS             3815..5248
                     /locus_tag="OO357_003479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anion permease"
                     /protein_id="HCR5888301.1"
                     /translation="MNKKSLWKLILILAIPCIIGFMPAPAGLSELAWVLFGIYLAAIV
                     GLVIKPFPEPVVLLIAVAASMVVVGNLSDGAFKTTAVLSGYSSGTTWLVFSAFTLSAA
                     FVTTGLGKLIAYLLIGKIGNTMLGLGYVTVFLDLVLAPATPSNTARAGGIVLPIINSV
                     AVALGSEPEKSPRRVGHYLMMSIYMVTKTTSYMFFTAMAGNILALKMINDILHLQISW
                     GGWALAAGLPGIIMLLVTPLVIYTMYPPEIKKVDNKTIAKAGLAELGPMKIREKMLLG
                     VFVLALLGWIFSKSLGVDESTVAIVVMATMLLLGIVTWEDEVKNKGGWNTLIWYGGII
                     GLSSLLSKVKFFEWLAEVFKNNLAFDGHGNVAFFVIIFLSIIVRYFFASGSAYIVAML
                     PVFAMLANVSGAPLMLTALALLFSNSYGGMVTHYGGAAGPVIFGVGYNDIKSWWLVGA
                     VLTILTFLVHITLGVWWWNMLIGWNML"
     gene            5431..7611
                     /locus_tag="OO357_003480"
     CDS             5431..7611
                     /locus_tag="OO357_003480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706815.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydratase"
                     /protein_id="HCR5888302.1"
                     /translation="MIKLSEKGVFLASNNEIIAEEHFTGEIKKEEAKKGTIAWSILSS
                     HNTSGNMDKLKIKFDSLASHDITFVGIVQTAKASGMERFPLPYVLTNCHNSLCAVGGT
                     INGDDHVFGLSAAQRYGGIFVPPHIAVIHQYMREMMAGGGKMILGSDSHTRYGALGTM
                     AVGEGGGELVKQLLNDTWDIDYPGVVAVHLTGKPAPYVGPQDVALAIIGAVFKNGYVK
                     NKVMEFVGPGVSALSTDFRNSVDVMTTETTCLSSVWQTDEEVHNWLALHGRGQDYCQL
                     NPQPMAYYDGCISVDLSAIKPMIALPFHPSNVYEIDTLNQNLTDILREIEIESERVAH
                     GKAKLSLLDKVENGRLKVQQGIIAGCSGGNYENVIAAANALRGQSCGNDTFSLAVYPS
                     SQPVFMDLAKKGVVADLIGAGAIIRTAFCGPCFGAGDTPINNGLSIRHTTRNFPNREG
                     SKPANGQMSAVALMDARSIAATAANGGYLTSASELDCWDNVPEYAFDVTPYKNRVYQG
                     FVKGATQQPLIYGPNIKDWPELGALTDNIVLKVCSKILDEVTTTDELIPSGETSSYRS
                     NPIGLAGNVSELTEVFARIKQIAGQEHIDPLQTEIGSMVYAVKPGDGSAREQAASCQR
                     VIGGLANIAEEYATKRYRSNVINWGMLPLQMAEVPTFEVGDYIYIPGIKAALDNPGTT
                     FKGYVIHEDAPVTEITLYMESLTAEEREIIKAGSLINFNKNRQM"
     gene            complement(7752..9035)
                     /locus_tag="OO357_003481"
     CDS             complement(7752..9035)
                     /locus_tag="OO357_003481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative acyl-CoA thioester hydrolase"
                     /protein_id="HCR5888303.1"
                     /translation="MNTFSVSRLALALAFGVTLTACSSTPPDQRPSDQTAPGTSSRPI
                     LSAKEALNFDAQHYFASLTPGAAAWNPSPITLPAQPDFVVGPAGTQGVTHTTIQAAVD
                     AAIIKRTNKRQYIAVMPGEYQGTVYVPAAPGGITLYGTGEKPIDVKIGLSLDGGMSPA
                     DWRHDVNPRGKYMPGKPAWYMYDSCQSKRSDSIGVLCSAVFWSQNNGLQLQNLTIENT
                     LGDSVDAGNHPAVALRTDGDQVQINNVNILGRQNTFFVTNSGVQNRLETNRQPRTLVT
                     NSYIEGDVDIVSGRGAVVFDNTEFRVVNSRTQQEAYVFAPATLSNIYYGFLAVNSRFN
                     ASGDGVAQLGRSLDVDANTNGQVVIRDSAINEGFNTAKPWADAVISNRPFAGNTGSVD
                     DNDEIQRNLNDTNYNRMWEYNNRGVGSKVVAEEKK"
     gene            complement(9170..>9550)
                     /locus_tag="OO357_003482"
     CDS             complement(9170..>9550)
                     /locus_tag="OO357_003482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5888304.1"
                     /translation="VEQSKTGVKIAIPTTLHVDALGISMKETLDKCKEILGGETIIAS
                     TRREPLSSGTVSRYFMRARKASGLSFEGDPPTFHELRSLSARLYEKQISDKFAQHLLG
                     HKSDTMASQYRDDRGREWDKIEIK"
BASE COUNT         2334 a         2231 c         2433 g         2552 t
ORIGIN      
        1 tttttttctt taaaaaaaca gtacattcta ttttataatg actgtgtatt cccatggaag
       61 gagatcaccc aattctcccc cccgactttt gacgcagtgt cttgttaaca gggggattga
      121 ccatgcttat tggctatgta cgcgtatcaa caaatgacca gaataccgat ctacaacgta
      181 atgcgtttat ttgtgccgga agtgagttga ttgttgcgcg aatgggaagt tggccagaag
      241 ttgttatggc atagtgttta cggtcgttga tacaggtaaa caaaagcggg gagagtcata
      301 ctccctgaat agtcgcgaaa atatctgtgg cattgtcaaa ttcgtgtcgg tgactaatat
      361 tttagtaata tgccattcga aatggagtgc taacttattg atttaattga tgtgcgatat
      421 tgtattttat cgtgttttgt tgtctgtttt ttatccagta agtgttgtta tatgtatgaa
      481 agataaggga tttatcattt ttgaggtgtg ttcatgaagc aaacagttta tatcgccagc
      541 cctgagagcc agcaaattca cgtctggaac ctgaatcatg aaggcgcact gacgctgaca
      601 caggttgtcg atgtgccggg gcaggtgcag ccgatggtgg tcagcccgga caaacgttat
      661 ctctatgttg gtgttcgccc tgagtttcgc gtcctggcgt atcgtattgc cccggacgat
      721 ggcgcactga cctttgccgc agagtctgtg ctgccgggta gtccgacgca tatttccacc
      781 gatcaccagg ggcagtttgt ctttgtaggt tcttacaatg cgggtaacgt gagcgtaacg
      841 cgtctggaag atggcctgcc agtgggcgtc gtcgatgtag tcgaggggct ggacggttgc
      901 cattccgcca atatctcacc ggacaaccgt acgctgtggg ttccggcatt aaagcaggat
      961 cgcatttgcc tgtttacggt cagcgatgat ggtcatctgg tggcgcagga ccctgcggaa
     1021 gtgactaccg ttgaaggggc cggcccgcgt catatggtat tccatccaaa cgaacaatat
     1081 gcgtattgcg tcaatgagtt aaacagctca gtggatgtct gggaactgaa agatccgcac
     1141 ggcaatatcg aatgtgtcca gacgctggat atgatgccgg aaaacttctc cgacacccgt
     1201 tgggcggctg atattcatat caccccggat ggtcgccatt tatacgcctg cgaccgtacc
     1261 gccagtctga ttaccgtttt cagcgtttcg gaagatggca gcgtgttgag taaagaaggc
     1321 ttccagccaa cggaaaccca gccgcgcggc ttcaatgttg attacagcgg caagtatctg
     1381 attgccgctg ggcaaaaatc tcaccacatc tcggtatacg aaattgttgg cgagcagggg
     1441 ctactgcatg aaaaaggccg ctatgcggtc gggcagggac caatgtgggt ggtggttaac
     1501 gcacactaac cgctgattta accggcgcag tctctcctgc gccggtgtat taacttatct
     1561 cctgtaacgc atgtctctgg cgttcgacga tattggtcca caaattgtct ttatcgtcag
     1621 tccacaaatt aatcagcaag gaaaaaaagc gttcagctgc cggagaaagc acggcatctt
     1681 tgcggcgaat aatccccaat gtccgacgta taacgggttc caccagtggg ataccaataa
     1741 tcgaagaata gggcgcatgg ggcattgcca gaccaggaag cgccgaaatc cccagtcctg
     1801 cctccactag tcctaatgac gtcgaaagat gacgcacttc gtaaaaccag tccagcttcc
     1861 agggcttgtc ggctagctgt tgttctatca acaatcggtt gccgctggag gaacgtacgc
     1921 caatcatttt gtaacccact aactcctgcc attctacaag ctactttttg gccaatggat
     1981 ggtcacgccg acaggccagc acgaatggct cgttgaccag tggggtaaaa tcaatggatg
     2041 aatttgtgac gttgttcatg tttatgccaa agtctgattc gttacacagc accgattcca
     2101 tgcaattatt cgtgccttgt tccagaatcc gcactttaat attgggatac agctcattaa
     2161 atttaccgat tgccagcggt aaaaaataaa atactgcggt cggaatacac gctaatgtca
     2221 ccataccacg atgataagca ttcatatcac gaatattaaa aagcgtttca tcgaatttct
     2281 ttattaattc ccgcgcctcc gggagcaacc tttttcctgc ttttgttaac gtaaccttgc
     2341 gagttgtacg ctcaaatagt tgaatatgta aatcttcctc catctttttt attcttcgcg
     2401 ttaatgcagg ttgcgtaata ttgagtaatt tagcggcatt gttaaatgaa ctggactctg
     2461 ccagtatgac aaatgccttc atacttgata attcatgctt cattattact ccggaaaatg
     2521 gaagcgacga ttttgggggg ctggtcgtta aaaattttaa ctgcatttag ccaacttaaa
     2581 ttaatgaaaa aatgttatta atcgttgagc taaagtcatt agagatgctt tgtccttaat
     2641 gtaaccatat cgcaataagt tatgttttta aattgagggc attattatga aaaaaatacc
     2701 ctgcgtgatg atgcgaggtg gaacctcgag gggcgcgttc ctgttagcgg aacatttacc
     2761 cgaagatcaa acgcagcgcg ataaaatatt gatggcaatt atgggttccg gtaacgatct
     2821 ggaaattgac ggtattggcg gcggtaatcc actgaccagt aaagtcgcca ttattagccg
     2881 ttccagcgat ccgcgtgctg atgtcgatta tctgtttgct caagttatcg tccatgagca
     2941 acgtgtcgat accacgccta actgcggcaa tatgctgtct ggcgtagggg cattcgccat
     3001 tgaaaatggt ttgattgcag cgacttcgcc agttacccgt gtacgtatcc gcaacgtcaa
     3061 tacgggtacg ttcatcgaag ctgatgtgca aacgccaaat ggtgttgtcg agtacgaggg
     3121 tagcgccaga attgacggcg taccgggtac tgccgcaccg gttgcgctca ctttcctgaa
     3181 tgccgctgga acgaaaactg gaaaagtttt cccgactgat aatcagattg attattttga
     3241 cgatgtcccg gtgacctgta tcgatatggc gatgccagtc gtcattattc cggctgaata
     3301 tctgggaaaa acaggttatg aattaccggc ggaactggat gccgacaaag cattattagc
     3361 ccgcattgaa tctatccggc tacaagcggg taaagcaatg ggcttaggtg atgtcagtaa
     3421 tatggttatc cctaaacctg tgcttatttc tccagcgcag aaaggcgggg caattaatgt
     3481 gcgttatttt atgccgcatt cttgccatcg cgcgctggcg ataaccggtg ctattgctat
     3541 ttccagtagt tgtgcattgg aaggcaccgt cacccgacaa atcgtccctt ctgtaggata
     3601 tggcaatatc aatattgaac accccagtgg tgcgctcgac gttcatttaa gtaatgaagg
     3661 tcaggatgcc acgacgttac gcgcatctgt tattcggaca accagaaaaa tattttccgg
     3721 tgaagtttat cttccctgaa aaaattcgtt gtcaggataa ggacaatcaa taaaggactt
     3781 ctgtatgagt catacagaaa gaacaggatt ttaaatgaat aagaaatcgt tatggaagct
     3841 aattctgata ttagcgatcc catgtattat tggttttatg ccagctccgg caggattaag
     3901 cgaactggcg tgggtgcttt ttggtattta cctggcggcc attgtgggtc tggttatcaa
     3961 gcctttcccg gaacctgtcg tactgttaat tgccgttgct gcctcaatgg tggtggtcgg
     4021 taacttatcc gacggtgcgt ttaagaccac cgccgtatta agcggttact cttcaggtac
     4081 tacctggctg gtgttctcgg cgtttacctt aagcgccgca tttgtgacca ccggtttagg
     4141 taaacttatt gcctatctgc tgattggtaa aatcggtaac accatgctgg gtctgggtta
     4201 cgttacggta ttcctcgatc tggtactggc tccggcaaca ccgtctaaca ccgcgcgtgc
     4261 gggcggcatt gtgttaccga tcatcaacag cgtggcggtg gctttggggt ccgaaccgga
     4321 aaaaagtccg cgtcgtgtcg gacattacct gatgatgtcc atttacatgg tcaccaaaac
     4381 caccagctat atgttcttta ccgcaatggc ggggaacatt ctggcgctga aaatgatcaa
     4441 cgacattctg cacctgcaaa ttagctgggg tggatgggcg ctggccgccg gattgccggg
     4501 catcattatg ctgctggtca ccccgctggt aatttacacc atgtatccac cagaaattaa
     4561 aaaggtggat aacaaaacca tcgctaaagc gggccttgcc gaactgggac cgatgaaaat
     4621 ccgcgaaaaa atgctgctcg gtgtctttgt gctggcgcta ctgggctgga ttttcagtaa
     4681 gtctctgggg gttgatgaat ccactgtggc aatcgttgtt atggcgacca tgctgctgct
     4741 gggtatcgtt acctgggaag acgaggttaa aaataaaggc ggctggaata ccttaatctg
     4801 gtacggcggt attatcggct taagctcctt attatcgaaa gttaaatttt tcgaatggtt
     4861 agctgaagtc tttaaaaata acctggcatt tgatggtcac ggtaacgttg ctttcttcgt
     4921 tattattttc ctcagcatca tcgtgcgtta tttcttcgct tccggtagtg cctatatcgt
     4981 tgctatgtta cctgtatttg ccatgctggc gaacgtctcc ggcgcaccgt taatgttaac
     5041 cgcgctggca ctgttgttct ccaactccta tggcggcatg gttactcact atggcggcgc
     5101 ggcaggtccg gtcatctttg gcgtgggtta caacgatatt aaatcctggt ggttggtcgg
     5161 tgcggtactg acgatattaa ccttcttggt gcatatcacc ctcggcgtgt ggtggtggaa
     5221 tatgctgatc ggctggaaca tgctgtaaat atactcgtca tacttcaagt tgcatgtgct
     5281 gcgtctgcgt tcgctcaccc cagtcactta cttatgtaag ctcctgggga ttcactcact
     5341 tgtcgccttc ctgcaactcg aattatttag agtatatcca tttattatct ttctgcgcac
     5401 ttcacggtgc gcagatatct ggagcatttg atgatcaagt tatctgaaaa aggcgtgttt
     5461 ctcgccagta ataacgaaat aattgccgaa gaacatttca ccggcgaaat taaaaaagaa
     5521 gaagcaaaaa aaggcactat tgcctggtct attctctctt ctcataatac gtccggaaat
     5581 atggataaac ttaaaattaa gtttgattca ttagcctctc acgatattac ctttgttggt
     5641 attgtacaga ccgctaaagc gtccggaatg gaacgtttcc cgctgccgta tgtgctgacc
     5701 aactgccata actcactctg cgccgtcggc ggcaccatta acggtgatga ccatgttttt
     5761 ggtttatcgg cagctcagcg ttatggcggt atttttgtgc ctccgcatat tgcggtcatc
     5821 catcaatata tgcgtgagat gatggctggc gggggcaaaa tgatcctcgg gtcagacagc
     5881 cacacccgtt acggtgcatt agggacaatg gcagtcggtg agggtggcgg tgagttggta
     5941 aaacagctgc ttaatgacac ctgggatatc gactatccgg gcgtggttgc ggtgcatctg
     6001 accggaaaac ctgcgccgta tgtggggcca caggatgtgg cgctggctat cattggcgcg
     6061 gtgttcaaaa acggttacgt caaaaacaaa gtcatggagt tcgttggacc gggcgttagc
     6121 gcgctctcta ccgatttccg taacagcgtt gacgtgatga ccacggaaac cacctgttta
     6181 agctccgtct ggcaaactga tgaagaagtg cataactggc tggcgctgca cggtcgcggt
     6241 caggattact gccagcttaa ccctcaaccg atggcgtact acgatggctg catcagcgtt
     6301 gatttaagcg ccatcaaacc aatgattgcg ctgccgttcc acccgagcaa cgtgtatgaa
     6361 atcgacacac tgaaccagaa cctgaccgac attctgcgtg agattgaaat tgagtccgaa
     6421 cgcgtggcgc acggtaaagc caaactctcg ctgctggata aagtggaaaa tggtcgcctg
     6481 aaagtgcagc aggggattat cgcgggctgt tctggcggta actacgaaaa cgtcatcgcg
     6541 gcggcgaatg cactgcgcgg tcaatcctgt ggcaatgaca ccttctcgct ggcggtttac
     6601 ccgtcatcac agccggtgtt tatggatctc gccaaaaaag gtgtggtagc agatttgatt
     6661 ggcgcaggcg caatcatcag aaccgcattc tgtggcccat gctttggcgc gggcgatacg
     6721 ccaatcaaca acggtttgag tattcgccac accacgcgta acttcccgaa ccgcgaaggc
     6781 tctaagccag ccaatgggca gatgtcagcg gtggcgttga tggacgctcg ttctatcgct
     6841 gcgactgcgg caaacggtgg ctatttaacc tctgccagcg aacttgattg ctgggacaac
     6901 gtgccggagt acgccttcga tgtaacgccg tataaaaacc gtgtttatca gggctttgtg
     6961 aaaggggcaa ctcagcaacc gctgatttac ggaccgaaca ttaaagactg gccggaattg
     7021 ggtgcgctga ctgacaatat cgtcctgaaa gtgtgctcga agatcctcga cgaagtcacc
     7081 accaccgacg aactgattcc ttccggtgaa acctcttctt atcgttcaaa tccgattggt
     7141 ctggcgggga atgtcagcga gctgacagag gtgtttgcgc gcattaagca gattgctggt
     7201 caggagcata ttgatccgct gcaaactgaa attggcagca tggtctatgc ggtgaaacca
     7261 ggcgatggtt ctgcgcgtga acaggcggcg agctgccagc gtgtgattgg cggtctggcg
     7321 aatattgccg aggagtacgc gactaaacgc taccgttcta acgtcatcaa ctgggggatg
     7381 ttaccgctgc agatggcgga agtgccaacc tttgaagtgg gggattacat ttacatccct
     7441 ggcattaaag cggcgctgga taatccgggt acgacgttta aaggttatgt gatccatgaa
     7501 gatgcgccgg taacggaaat tacgctttac atggaaagcc tgacggctga agagcgcgag
     7561 attatcaagg cgggtagttt gattaacttc aataaaaacc gtcagatgta aaaagcgcca
     7621 tgtgaatgta ggtcgcattc ggcacttatt gtcggatgcg atgctggcgc atcttatccg
     7681 acctacaaat cgcatcgaac cgtaggccgg ataaggcgtt tacgccgcat ccggcaaata
     7741 gttaatagct cttacttctt ctcctctgca accactttgc tgcccacgcc gcggttattg
     7801 tattcccaca tgcggttgta gttagtgtca ttcagattgc gctgtatttc gtcgttatca
     7861 tctacgctgc cggtattacc cgcaaacgga cgattagaga tcaccgcatc ggcccacggt
     7921 ttagccgtgt taaaaccttc gttgatggcg ctatcacgga tcaccacctg accgttggta
     7981 ttagcatcaa catccagcga gcggcccagt tgcgccacgc catcaccgga agcattgaaa
     8041 cggctgttta cggcgaggaa accgtaatag atgttagaca gcgtagccgg tgcaaacaca
     8101 tatgcttctt gctgggtacg tgagttcacc acgcggaatt cggtgttatc gaacaccact
     8161 gcgccgcgac cagaaacgat atccacatcc ccttcaatat agctgttggt caccagcgta
     8221 cgcggctgac ggttggtttc cagacggttc tgcacaccgc tgttggtgac aaagaaggtg
     8281 ttctgacgac cgagaatgtt aacgttgtta atctgtactt ggtcaccgtc agtacgcagt
     8341 gctaccgccg gatggttacc tgcatctacg ctatcgccca gcgtgttttc gatggtcaga
     8401 ttttgcagtt gcagtccatt gttttgtgac cagaagaccg cagaacacag aacaccgata
     8461 ctgtcgctgc gtttgctctg gcagctatcg tacatatacc acgctggttt acctggcata
     8521 tatttgccgc gcgggttgac gtcgtgacgc cagtcggcag ggctcatgcc cccatcaagg
     8581 gaaagcccaa tcttcacatc aatcggtttt tcacctgtac cgtacagagt gattccacct
     8641 ggagcagcag ggacatacac cgttccctga tactcaccag gcatcacggc aatatactgg
     8701 cgcttgttgg tgcgcttgat aattgccgca tctaccgccg cctgaatcgt ggtatgcgtt
     8761 acaccttgag tacccgccgg gccgacaaca aagtcaggtt gcgcaggcag ggttatcggg
     8821 gacggattcc acgctgcagc acctggtgtc agggatgcaa aatagtgttg cgcatcgaaa
     8881 ttcagcgctt cttttgccga cagaatcggg cgagaagagg taccaggcgc agtttgatca
     8941 gaaggacgtt gatcgggtgg tgttgagcta caggcggtca gcgtcacgcc aaaagccaat
     9001 gccagcgcca gacgggaaac tgaaaatgtg ttcacaggtt gctccgggct atgaaataga
     9061 aaaatgaatc cgttgaagcc tgctttttta tactaagttg gcattataaa aaagcattgc
     9121 ttatcaattt gttgcaacga acaggtcatt atcagtcaaa ataaaatcat tatttgattt
     9181 caattttgtc ccactccctg cctctgtcat cacgatactg tgatgccatg gtgtccgact
     9241 tgtgcccgag aagatgttga gcaaacttat cgcttatctg cttctcatag agtcttgcag
     9301 acaaactgcg caactcgtga aaggtaggtg gatccccttc gaaggaaaga cctgatgctt
     9361 ttcgtgcgcg cataaaatac cttgatactg tgccggatga aagcggttca cgacgagtag
     9421 atgcaattat ggtttctccg ccaagaatct ctttgcattt atcaagtgtt tccttcattg
     9481 atatcccgag agcatcaaca tgcaatgttg tagggatggc aatttttacg cctgttttgc
     9541 tttgctcgac
//
LOCUS       DAJTRC010000125         9509 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.125, whole genome shotgun sequence.
ACCESSION   DAJTRC010000125 DAJTRC010000000
VERSION     DAJTRC010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9509
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.125"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(150..890)
                     /gene="pflA"
                     /locus_tag="OO357_003483"
     CDS             complement(150..890)
                     /gene="pflA"
                     /locus_tag="OO357_003483"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase 1-activating protein"
                     /protein_id="HCR5888305.1"
                     /translation="MSVIGRIHSFESCGTVDGPGIRFITFFQGCLMRCLYCHNRDTWD
                     THGGKEVTVEDLMKEVVTYRHFMNASGGGVTASGGEAILQAEFVRDWFRACKKEGIHT
                     CLDTNGFVRRYDPVIDELLEVTDLVMLDLKQMNDEIHQNLVGVSNHRTLEFAKYLANK
                     NVKVWIRYVVVPGWSDDDDSAHRLGEFTRDMGNVEKIELLPYHELGKHKWVAMGEEYK
                     LDGVKPPKKETMERVKGILEQYGHKVMF"
     gene            complement(1082..3364)
                     /gene="pflB"
                     /locus_tag="OO357_003484"
     CDS             complement(1082..3364)
                     /gene="pflB"
                     /locus_tag="OO357_003484"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_459948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HCR5888306.1"
                     /translation="MSELNEKLATAWEGFTKGDWQNEVNVRDFIQKNYTPYEGDESFL
                     AGATEATTTLWDKVMEGVKLENRTHAPVDFDTAVASTITSHDAGYINKALEKVVGLQT
                     EAPLKRALIPFGGIKMIEGSCKAYNRELDPMIKKIFTEYRKTHNQGVFDVYTPDILRC
                     RKSGVLTGLPDAYGRGRIIGDYRRVALYGIDYLMKDKYAQFTSLQADLENGVNLEQTI
                     RLREEIAEQHRALGQMKEMAAKYGYDISGPATNAQEAIQWTYFGYLAAVKSQNGAAMS
                     FGRTSTFLDVYIERDLKAGKITEQEAQEMVDHLVMKLRMVRFLRTPEYDELFSGDPIW
                     ATESIGGMGLDGRTLVTKNSFRFLNTLYTMGPSPEPNMTILWSEKLPLNFKKFAAKVS
                     IDTSSLQYENDDLMRPDFNNDDYAIACCVSPMIVGKQMQFFGARANLAKTMLYAINGG
                     VDEKLKMQVGPKSEPIKGDLLSYDEVMERMDHFMDWLAKQYITALNIIHYMHDKYSYE
                     ASLMALHDRDVIRTMACGIAGLSVAADSLSAIKYAKVKPIRDEDGLAIDFEIEGEYPQ
                     FGNNDPRVDDLAVDLVERFMKKIQKLHTYRDAIPTQSVLTITSNVVYGKKTGNTPDGR
                     RAGAPFGPGANPMHGRDQKGAVASLTSVAKLPFAYAKDGISYTFSIVPNALGKDDEVR
                     KTNLAGLMDGYFHHEASIEGGQHLNVNVMNREMLLDAMENPEKYPQLTIRVSGYAVRF
                     NSLTKEQQQDVITRTFTQSM"
     gene            complement(3419..4276)
                     /gene="focA"
                     /locus_tag="OO357_003485"
     CDS             complement(3419..4276)
                     /gene="focA"
                     /locus_tag="OO357_003485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706822.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate transporter FocA"
                     /protein_id="HCR5888307.1"
                     /translation="MKADNPFDLLLPAAMAKVAEEAGVYKATKHPLKTFYLAITAGVF
                     ISIAFVFYITATTGTGTMPFGMAKLVGGICFSLGLILCVVCGADLFTSTVLIVVAKAS
                     GRITWCQLAKNWLNVYFGNLVGALLFVLLMWLSGEYMTANGQWGLNVLQTADHKVHHT
                     FIEAVCLGILANLMVCLAVWMSYSGRSLMDKAFIMVLPVAMFVASGFEHSIANMFMIP
                     MGIVIRDFASPEFWTAVGSAPENFSHLTVMNFITDNLIPVTIGNIIGGGLLVGLTYWV
                     IYLRENDHH"
     gene            complement(4683..6443)
                     /locus_tag="OO357_003486"
     CDS             complement(4683..6443)
                     /locus_tag="OO357_003486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcaO-like family protein"
                     /protein_id="HCR5888308.1"
                     /translation="MTQTFIPGKDAALEDSIARFQQKLSDLGFQIEEASWLNPVPNVW
                     SVHIRDKECALCFTNGKGATKKAALASALGEYFERLSTNYFFADFWLGETIANGPFVH
                     YPNEKWFPLSENDDVPEGLLDDRLRAFYDPENELTGSMLIDLQSGNEDRGICGLPFTR
                     QSDNQTVYIPMNIIGNLYVSNGMSAGNTRNEARVQGLSEVFERYVKNRIIAESISLPE
                     IPADVLARYPAVVEAIETLETEGFPIFAYDGSLGGQYPVICVVLFNPANGTCFASFGA
                     HPDFGVALERTVTELLQGRGLKDLDVFTPPTFDDEEVAEHTNLETHFIDSSGLISWDL
                     FKQDADYPFVDWNFSGTTEEEFATLMAIFNKEDKEVYIADYEHLGVYACRIIVPGMSD
                     IYPAEDLWLANNSMGSHLRETILSLPGSEWEKEDYLNLIEQLDEEGFDDFTRVRELLG
                     LATGSDNGWYTLRIGELKAMLALAGGDLEQALVWTEWTMEFNSSVFSPERANYYRCLQ
                     TLLLLAQEEDRQPLQYLNAFVRMYGADAVEAASAAMSGEAAFYGLQPVDSDLHAFAAH
                     QSLLKAYEKLQRAKAAFWAK"
     gene            6573..7199
                     /locus_tag="OO357_003487"
                     /pseudo
     CDS             6573..7199
                     /locus_tag="OO357_003487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415426.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
     gene            7398..8486
                     /gene="serC"
                     /locus_tag="OO357_003488"
     CDS             7398..8486
                     /gene="serC"
                     /locus_tag="OO357_003488"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HCR5888309.1"
                     /translation="MAQIFNFSSGPAMLPAEVLKQAQQELRDWNGLGTSVMEVSHRGK
                     EFIQVAEEAEKDFRDLLNVPSNYKVLFCHGGGRGQFAAVPLNILGDKTTADYVDAGYW
                     AASAIKEAKKYCTPNVFDAKVTVDGLRAVKPMREWQLSDNAAYMHYCPNETIDGIAID
                     ETPDFGKDVVVAADFSSTILSRPIDVSRYGVIYAGAQKNIGPAGLTIVIVREDLLGKA
                     NIACPSILDYSILNDNGSMFNTPPTFAWYLSGLVFKWLKANGGVAEMDKINQQKAELL
                     YGVIDNSDFYRNDVAKANRSRMNVPFQLADSALDKLFLEESFAAGLHALKGHRVVGGM
                     RASIYNAMPLEGVKALTDFMVEFERRHG"
     gene            8557..>9509
                     /gene="aroA"
                     /locus_tag="OO357_003489"
     CDS             8557..>9509
                     /gene="aroA"
                     /locus_tag="OO357_003489"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5888310.1"
                     /translation="MESLTLQPIARVDGTINLPGSKSVSNRALLLAALAHGKTVLTNL
                     LDSDDVRHMLNALTALGLSYTLSADRTRCEIIGNGGPLHAEGALELFLGNAGTAMRPL
                     AAALCLDSNDIVLTGEPRMKERPIGHLVDALRLGGAKITYLEQENYPPLRLQGGFTGG
                     NVDVDGSVSSQFLTALLMTAPLAPEDTVIRIKGDLVSKPYIDITLNLMKTFGVEIENQ
                     HYQQFVVKGGQSYQSPGTYLVEGDASSASYFLAAAAIKGGTVKVTGIGRNSMQGDIRF
                     ADVLEKMGATICWGDDYISCTRGELNAIDMDMNHIPDAAMT"
BASE COUNT         2414 a         2272 c         2379 g         2444 t
ORIGIN      
        1 aagaacttca cgcgagctat tatcagtcgt tattatctca ggtacgggat atggtagatg
       61 cactataaga tgtgttaaaa acgctgtagc agaatgaagc gcggaataaa aaagcggcaa
      121 ctcaataaag ttgccgcttt acgaggaaat tagaacatta ccttatgacc gtactgctca
      181 agaatgcctt tcacgcgttc catggtctct ttcttcggtg gtttaacacc atcgagtttg
      241 tattcttcac ccatcgccac ccatttgtgt ttgcccagtt cgtggtaggg gagaagttcg
      301 attttctcaa cgttacccat atcacgggta aattcaccga ggcgatgtgc cgaatcgtca
      361 tcgtcagacc agcctgggac aacaacgtag cggatccaca ccttcacatt tttgttcgcc
      421 agatatttag cgaactccag cgtgcggtgg ttggaaactc caaccagatt ttggtggatc
      481 tcgtcgttca tctgtttgag atcgagcatt accaggtcgg ttacttccag cagttcatca
      541 atcaccggat cgtaacgacg aacaaaaccg ttggtatcca gacaggtatg aatgccttct
      601 tttttacagg cgcggaacca gtcacgaaca aactcagctt gcaggattgc ttcaccgccg
      661 gatgcggtaa cgccgccgcc ggaagcgttc ataaagtggc gataggtcac cacttccttc
      721 atcaaatctt caacggtaac ttctttaccg ccatgcgtgt cccaggtgtc gcggttatga
      781 caatacaggc agcgcatcag gcagccctgg aaaaaggtga taaagcgaat acctgggccg
      841 tctacggttc cacaggattc aaaggagtga atgcgaccaa taactgacat tgcggtgttt
      901 ctccagatgt ggcccatctg aggccgtgtt ggtgcgcagc tcgaaggcta cgtcgagtct
      961 gttttggcag tcaccttaaa gtatagatag ctgacaaaaa aggctctcgt gctaaaaaag
     1021 gccccacttt cgtggagcct ttattgtacg ctttttactg tacgatttca gtcaaatcta
     1081 attacataga ttgagtgaag gtacgagtaa taacgtcctg ctgctgttct ttagtcagcg
     1141 agttgaaacg tactgcgtag ccagatacac ggatggtcag ctgcggatat ttttccgggt
     1201 tttccatcgc gtcgagcagc atttcacggt tcatcacgtt aacgttcagg tgctgaccac
     1261 cttcgatgga tgcttcgtgg tggaagtaac catccatcag accagccagg ttggtcttac
     1321 gaacttcgtc gtctttaccc agtgcgttcg gaacgataga gaaggtgtag gagataccat
     1381 ctttagcgta agcaaacggc agtttagcaa cggaagtcag agacgctaca gcacctttct
     1441 ggtcacgacc gtgcatcggg ttagcacccg gtccgaacgg cgcgccagca cgacgaccgt
     1501 ctggggtgtt accagttttc ttaccataca caacgttaga agtgatggtc agaacagact
     1561 gagtcgggat agcgtcacgg taggtgtgca gtttctgaat tttcttcatg aaacgttcta
     1621 ccaggtcaac agccaggtca tctacacgcg gatcattgtt accaaactgc gggtattcgc
     1681 cttcgatttc gaagtcgata gccagaccgt cttcgtcacg aatcggttta actttcgcat
     1741 atttgattgc agacagggag tcagcagcaa cggacagacc agcgatacca cacgccatgg
     1801 tgcggataac gtcacggtcg tgcagcgcca tcagagaggc ttcgtagctg tacttgtcgt
     1861 gcatgtagtg gatgatgttc agtgcagtga tgtactgttt agccagccag tccatgaagt
     1921 gatccatgcg ctccatcact tcatcgtagc tcaggagatc gcctttgatc ggttcagact
     1981 tcggaccaac ctgcattttc agtttttcgt caacgccgcc gttgattgcg tacagcatgg
     2041 ttttcgccag gtttgcacgc gcaccgaaga actgcatttg tttaccaacg atcatcgggc
     2101 ttacgcagca agcgatagcg tagtcatcgt tgttgaagtc cggacgcatc aggtcatcgt
     2161 tctcatactg cagagaagag gtgtcgatgg acactttagc ggcgaatttc ttgaagttca
     2221 gcggcagttt ttcagaccac agaatggtca tgttcggttc cggagacggc cccatagtgt
     2281 acagggtgtt caggaaacgg aagctgtttt tggtaaccag ggtacgacca tcgaggccca
     2341 taccaccgat agattcggtt gcccagattg ggtcgccaga gaacagttca tcgtattccg
     2401 gagtacgcag gaagcgaacc atacgcagtt tcatgaccag gtggtcaacc atttcctgcg
     2461 cttcttgttc ggtgatcttg ccagctttca ggtcacgttc gatgtacaca tccaggaagg
     2521 tggaggtacg accgaaggac attgcagcac cgttctgaga cttaacagca gccaggtagc
     2581 cgaagtaagt ccactggata gcttcctgag cgttggtagc cggaccagag atgtcgtagc
     2641 cgtatttcgc agccatttct ttcatctgac ccagagcgcg gtgctgttca gcgatttctt
     2701 cgcgcagacg gatagtctgt tccaggttta cgccgttttc cagatcagcc tgtagagagg
     2761 tgaactgagc gtatttgtct ttcatcaggt agtcgatacc gtacagcgca acgcgacggt
     2821 agtcaccgat gatacggcca cggccataag catctggcag accggtcaga acaccggatt
     2881 tacggcaacg caggatgtcc ggagtgtaaa cgtcgaacac gccctggttg tgagttttac
     2941 ggtattcagt gaagattttt ttgatcatcg ggtccagttc gcggttgtac gctttgcagg
     3001 aaccttcgat cattttgata ccaccgaacg ggataagagc acgtttcagc ggagcttcag
     3061 tctgcagacc aacaactttt tccaacgctt tattgatgta gccagcgtcg tgagaggtga
     3121 tggtggaagc aacagcggtg tcaaaatcaa ctggcgcgtg agtgcggttt tccagtttaa
     3181 caccttccat tactttgtcc cacagggtgg tggtcgcttc agtagcgcca gccaggaagg
     3241 actcgtcacc ctcgtacgga gtgtagtttt tctgaatgaa gtcacggacg tttacttcat
     3301 tctgccagtc acctttggta aaaccttccc aggctgtggc taacttttca ttaagctcgg
     3361 acatgtaaca cctaccttct taagtggatt ttttatttac tgcgtacttc gacaaccatt
     3421 aatggtgatc gttttcacgc aggtaaatga cccagtatgt caacccaacc aacaaaccac
     3481 cgccgataat attaccgatc gtaaccggga tcaggttatc agtgatgaag ttcatcacgg
     3541 tcaggtgaga aaaattttcc ggtgcagaac cgacagcggt ccagaattcc ggagatgcga
     3601 agtcgcggat tacaataccc atcgggatca taaacatgtt tgcgatactg tgctcaaaac
     3661 cgctggcaac aaacatcgcg accggcagca ccataatgaa cgctttgtcc atcaggctgc
     3721 ggccagaata actcatccat actgccagac ataccatcag gtttgccagg ataccaagac
     3781 agacggcctc aataaaagta tggtgcactt tgtggtcggc ggtttgtagg acgtttagtc
     3841 cccattgacc atttgcggtc atatactcgc cggaaagcca cattaaaagt acaaacagca
     3901 gtgcgccgac caggttgcca aaatagacat ttagccagtt tttagccaac tgacaccagg
     3961 tgatgcgccc actcgcctta gcaacaacaa tcaacactgt ggaagtaaag agatcggctc
     4021 cgcagacaac acaaagaatc agccccagag agaagcaaat gccgccaacc agttttgcca
     4081 tgccgaaggg cattgtgcct gtgccagtgg ttgctgtgat atagaagacg aatgcgattg
     4141 agatgaaaac accggcggta atcgccagat agaaagtctt aagcggatgt ttcgttgctt
     4201 tatagacacc cgcctcttcg gccactttgg ccattgcagc aggaagtaaa agatcaaaag
     4261 ggttgtcagc tttcacacta actctctctt tattaagtcg gcgacgagat actaacaaag
     4321 cattatagat gagaaattga tatagatcat atctcgcctg gcttataggc ccgtaactcg
     4381 catggttttt atgcaaatac ggagtaacta tttgattatc caaaataaaa ataaatttta
     4441 aaaattaaca aatgagttga attttttccg catcctccgc taaaacagtt aattaaaagg
     4501 gagcatcagg cgaataaagt aacaatatcg atcgtattta ttaaatacaa attaccgata
     4561 tttaacctta taattacaat tattttatta atgcaaatat atgtaaagcg gggcattaaa
     4621 aaaacgcccc gtaatataac tcagactaat cattaagcct acattgcgta ggctatttga
     4681 ttttattttg cccagaatgc tgctttggcg cgctgcagct tttcgtaggc cttcaacaac
     4741 gactgatgtg cagcaaacgc gtgcagatcg ctatctaccg gttgcaggcc gtaaaacgcc
     4801 gcttcgccgc tcattgccgc actggcggct tctacggcat ctgcgccgta catgcgaaca
     4861 aacgcattca gatattgcag cggctggcga tcttcttcct gtgccagtaa taacaacgtt
     4921 tgcaggcagc gataatagtt ggcgcgttcc ggactaaata ctgatgagtt aaactccatc
     4981 gtccattcgg tccagaccag agcctgttcc agatcgccac cagccagcgc cagcatggct
     5041 tttaattcac cgatacgcag ggtgtaccaa ccgttatccg acccggtcgc cagacccaac
     5101 agctcacgca cgcgggtaaa gtcatcaaaa ccttcttcat ccagttgctc gatgaggttc
     5161 aggtaatctt ctttttccca ctcgctgcct ggtagcgaaa gaatcgtttc acgtaaatgg
     5221 ctgcccatac tgttattcgc gagccacaga tcttcagccg gataaatatc ggacatgcca
     5281 ggcacgataa tacggcaagc ataaacgccc agatgctcgt aatcggcaat ataaacttct
     5341 ttatcttctt tgttgaagat agccatcagc gtagcaaact cttcttccgt ggtgccggag
     5401 aaattccagt ccacaaacgg ataatcggca tcctgcttga acaggtccca ggagattaaa
     5461 ccgctggaat cgataaagtg cgtttcgagg ttggtatgtt cagcgacttc ttcatcatcg
     5521 aaggttggcg gagtaaacac atccaaatct ttcaggccac gaccttgcag cagctcggtc
     5581 acggtacgtt ccagtgctac gccaaaatca ggatgcgcac cgaaagaggc aaagcaggtg
     5641 ccgtttgcag gattaaacag taccacgcaa atcaccggat actggccgcc aagggaacca
     5701 tcatatgcga agatcgggaa accttctgtt tccagtgttt cgatcgcttc aaccaccgct
     5761 gggtaacgcg ccagcacgtc tgccgggatt tccggcaagc tgatgctttc agcaataatg
     5821 cggtttttca cgtagcgttc gaaaacttcg gacaacccct gaacgcgtgc ttcgttgcgg
     5881 gtattgccag cggacatacc gttagaaacg tacaggttac caatgatatt catcggaata
     5941 taaacggtct gattgtcgga ctggcgcgta aacggcagac cgcaaatacc acgatcttcg
     6001 ttaccggatt gtaggtcaat cagcatactg ccggtcaatt cattctccgg atcgtaaaat
     6061 gcgcgcagac ggtcatcgag cagcccttct ggcacatcgt cattttcgct cagtgggaac
     6121 catttttcgt tgggataatg cacaaatgga ccgttggcga tggtttcgcc cagccagaaa
     6181 tcagcaaaaa agtagttggt tgagagacgc tcgaaatatt caccgagtgc agaagccagc
     6241 gccgctttct tggttgcgcc tttaccgttg gtaaaacaca gtgcgcactc tttgtcgcga
     6301 atatgtacag accagacgtt aggcacggga ttcagccagg aggcctcttc aatctgaaag
     6361 ccgaggtctg aaagtttttg ctggaagcga gcgatggaat cttccagagc ggcatctttg
     6421 ccggggataa atgtttgcgt catgaaaatc actttagtcg tacggaaagc gcgcaataat
     6481 acgggtttta tctcaaaggc gctatcaccg ccgccatgcc gatgaactgt tgactatgct
     6541 tttagcggat aaaccacgtt aagagcataa aaatgaaggc gttcgatctc caccgtatgg
     6601 catttgataa agtgcctttt gatttccttg gcgaagttgc actacgtagt ctttatacct
     6661 ttgtactggt tttttgttcc tcaaaatgac cggaagacgc ggtgtgcgac agatgtcgct
     6721 gtttgaagtg ttaatcattc tcacgctggg ttcagcggcg ggagatgtgg cgttttatga
     6781 tgatgtgccg atggtgccgg tacttatcgt ctttatcact ctggcgttgt tataccgcct
     6841 ggtaatgtga tggcgagctg gcctggtcga aactcaataa ctccaacatg acggaatttg
     6901 agttctttat ggagctacga ttgcgtggcg tggagcagct ggggcaggta cgtctggcga
     6961 ttctcgaaac caacgggcaa atcagtgtct atttctttga agatgacaag gtgaaaccgg
     7021 gtttacttat tttacccagt gattgtacgc agcgttacaa agtggtgccg gagtcggcgg
     7081 actatgcctg cattcgttgt agtgaaatca ttcatatgaa cgcgggggaa aaacaattat
     7141 gtccgcgctg tgcaaatcca gaatggacga aggcaagtcg ggcaaaacgg gtgacctgac
     7201 agtaaaaaca tcggcttttt gctaataatc cgagagattc ttttgtgtga tgcaagccac
     7261 atttttgccc tcaacggttt tactcattgc gatgtgtgtc actgaatgat aaaaccgata
     7321 gccacaggaa taatgtatta cctgtggtcg caatcgattg accgcgggtt aatagcaacg
     7381 caacgtggtg aggggaaatg gctcaaatct tcaattttag ttctggtccg gcaatgctac
     7441 cggcagaggt gcttaaacag gctcaacagg aactgcgcga ctggaacggt cttggtacgt
     7501 cggtgatgga agtgagtcac cgtggcaaag agttcattca ggttgcagag gaagccgaga
     7561 aggattttcg cgatcttctt aatgtcccct ccaactacaa ggtattattc tgccatggcg
     7621 gtggtcgcgg tcagtttgct gcggtaccgc tgaatattct cggtgataaa accaccgcag
     7681 attatgttga tgccggttac tgggcggcaa gtgccattaa agaagcgaaa aaatactgca
     7741 cgcctaatgt ctttgacgcc aaagtgactg ttgatggtct gcgcgcggtt aagccaatgc
     7801 gtgaatggca actctctgat aatgcggctt atatgcatta ctgcccgaat gaaaccatcg
     7861 acggtatcgc catcgacgag acgccagact tcggcaaaga tgtggtggtc gccgccgact
     7921 tctcttcaac cattctttcc cgtccgattg acgtcagccg ttatggtgta atttacgctg
     7981 gcgcgcagaa aaatatcggc ccggctggcc tgacaatcgt catcgttcgt gaagatttgc
     8041 tgggcaaagc gaatatcgcg tgtccgtcga ttctggatta ttccatcctc aacgataacg
     8101 gctccatgtt taacacgccg ccgacatttg cctggtatct atctggtctg gtctttaaat
     8161 ggctgaaagc gaacggcggt gtagctgaaa tggataaaat caatcagcaa aaagcagaac
     8221 tgctatatgg ggtgattgat aacagcgatt tctaccgcaa tgacgtggcg aaagctaacc
     8281 gttcgcggat gaacgtgccg ttccagttgg cggacagtgc gcttgacaaa ttgttccttg
     8341 aagagtcttt tgctgcaggc cttcatgcac tgaaaggaca ccgtgtggtc ggcggaatgc
     8401 gcgcgtctat ttataatgcc atgccgctgg aaggcgttaa agcgctgaca gacttcatgg
     8461 ttgagttcga acgccgtcac ggttaatgcc gaaattttgc ttaatcccca cagccagcct
     8521 gtggggtttt tatttctgtt gtagagagtt gagttcatgg aatccctgac gttacaaccc
     8581 atcgctcgtg tcgatggcac tattaatctg cccggttcca agagcgtttc taaccgcgct
     8641 ttattgcttg cggcattagc acacggcaaa acagtattaa ccaatctgct ggatagcgac
     8701 gacgtgcgcc atatgctgaa tgcattaaca gcgttagggt taagctatac gctttcagcc
     8761 gatcgtacgc gttgcgaaat tatcggtaac ggcggtccat tacacgcaga aggtgccctg
     8821 gagttgttcc tcggtaacgc cggaacggca atgcgtccgc tggcggcagc tctttgtctg
     8881 gatagcaatg atattgtgct gaccggtgag ccgcgtatga aagagcgccc gattggtcat
     8941 ctggtggatg cgctgcgcct gggcggggcg aagatcactt acctggaaca agaaaattat
     9001 ccgccgttgc gtttacaggg cggctttact ggcggcaacg ttgacgttga tggctccgtt
     9061 tccagccaat tcctcaccgc actgttaatg actgcgcctc ttgcgccgga agatacggtg
     9121 attcgtatta aaggcgatct ggtttctaaa ccttatatcg acatcacact caatctgatg
     9181 aagacgtttg gtgttgaaat tgaaaatcag cactatcaac aatttgtcgt aaaaggcggg
     9241 cagtcttatc agtctccggg tacttatttg gtcgaaggcg atgcatcttc ggcttcttac
     9301 ttcctggcag cagcagcaat caaaggcggc actgtaaaag tgaccggtat tggacgtaac
     9361 agtatgcagg gtgatattcg ctttgctgat gtgctggaaa aaatgggcgc gaccatttgc
     9421 tggggcgatg attatatttc ctgcacgcgt ggtgaactga acgctattga tatggatatg
     9481 aaccatattc ctgatgcggc gatgaccat
//
LOCUS       DAJTRC010000126         9483 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.126, whole genome shotgun sequence.
ACCESSION   DAJTRC010000126 DAJTRC010000000
VERSION     DAJTRC010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9483)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9483)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9483
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.126"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..179)
                     /locus_tag="OO357_003490"
     CDS             complement(<1..179)
                     /locus_tag="OO357_003490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888311.1"
                     /translation="MNMMRIIYIGLSGVGMMFSSMASGHDAGGLQSPTCGVVCDPYIC
                     VNSDGISPELTRKYLG"
     gene            699..1394
                     /gene="minC"
                     /locus_tag="OO357_003491"
     CDS             699..1394
                     /gene="minC"
                     /locus_tag="OO357_003491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HCR5888312.1"
                     /translation="MSNTPIELKGSSFTLSVVHLHEAEPKVIHQALKDKIAQAPTFLK
                     HAPVVLNVSALEDPVNWSAMHKAVSATGLRVIGVSGCKDAQLKAEIEKMGLPILTEGK
                     EKAPRPAPAPQAPAQNTMPVTKTRLIDTPVRSGQRIYAPQCDLIVTSHVSAGAELIAD
                     GNIHVYGMMRGRALAGASGDRETQIFCTNLMAELVSIAGEYWLSDQIPAEFYGKAARL
                     QLVENALTVQPLN"
     gene            1418..2230
                     /gene="minD"
                     /locus_tag="OO357_003492"
     CDS             1418..2230
                     /gene="minD"
                     /locus_tag="OO357_003492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020801817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HCR5888313.1"
                     /translation="MARIIVVTSGKGGVGKTTSSAAIATGLAQKGKKTVVIDFDIGLR
                     NLDLIMGCERRVVYDFVNVIQGDATLNQALIKDKRTENLYILPASQTRDKDALTREGV
                     AKVLDDLKAMDFEFIVCDSPAGIETGALMALYFADEAIITTNPEVSSVRDSDRILGIL
                     ASKSRRAENGEEPIKEHLLLTRYNPGRVSRGDMLSMEDVLEILRIKLVGVIPEDQSVL
                     RASNQGEPVILDINADAGKAYADTVERLLGEERPFRFIEEEKKGFLKRLFGG"
     gene            2234..2500
                     /gene="minE"
                     /locus_tag="OO357_003493"
     CDS             2234..2500
                     /gene="minE"
                     /locus_tag="OO357_003493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HCR5888314.1"
                     /translation="MALLDFFLSRKKNTANIAKERLQIIVAERRRSDAEPHYLPQLRK
                     DILEVICKYVQIDPEMVTVQLEQKDGDISILELNVTLPEAEELK"
     gene            complement(2872..3216)
                     /locus_tag="OO357_003494"
                     /pseudo
     CDS             complement(2872..3216)
                     /locus_tag="OO357_003494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311542.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
     gene            complement(3251..3370)
                     /locus_tag="OO357_003495"
     CDS             complement(3251..3370)
                     /locus_tag="OO357_003495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001131457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HCR5888315.1"
                     /translation="MPLSYAGYHHFIMMSLVSGEHNTPALSLQDESPGLVPAG"
     gene            complement(3331..3516)
                     /locus_tag="OO357_003496"
     CDS             complement(3331..3516)
                     /locus_tag="OO357_003496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888316.1"
                     /translation="MSRRYSSATITLVILFFRRPHCLKISVRQYYLKRVIFYPTTLQR
                     TACKKCLFLMRDIIISS"
     gene            3617..3790
                     /locus_tag="OO357_003497"
     CDS             3617..3790
                     /locus_tag="OO357_003497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888317.1"
                     /translation="MSYSSFKIILIHVKNIIPIITATLILNYLNNSERSLVKQILIED
                     EIIVCATYLIPDI"
     gene            3792..4136
                     /locus_tag="OO357_003498"
     CDS             3792..4136
                     /locus_tag="OO357_003498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415690.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine zipper family protein"
                     /protein_id="HCR5888318.1"
                     /translation="MKKKILAFGLISALFCSTPAIADMNRTTKGALLGAGVGLLTGNG
                     VNGVLKGAAVGAGVSAVTEKGRDGKNARKGAKVGAAVGAVTGVLTGNGLEGAIKGAVI
                     GGTGGAILGKMK"
     gene            4140..4475
                     /locus_tag="OO357_003499"
     CDS             4140..4475
                     /locus_tag="OO357_003499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YmgD family protein"
                     /protein_id="HCR5888319.1"
                     /translation="MMMKKFALLAGLFVFAPMTWAQDYNIKNGLPSETYITCAEANEM
                     AKTDSAQVAEIVAVMGNASVASRDLKIEQSPELSAKVVEKLNQVCAKDPQMLLITAID
                     DTMRAIGKK"
     gene            complement(4532..6853)
                     /locus_tag="OO357_003500"
     CDS             complement(4532..6853)
                     /locus_tag="OO357_003500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter outer membrane beta-barrel
                     domain-containing protein"
                     /protein_id="HCR5888320.1"
                     /translation="MQGKNTIVTTGDYSIGLLSQTSGNLNTDTIIRVNSDGSVTPSFS
                     DGDDTFIVTAGNHAVGVLACASPGSACACVSSLDEESTADTGSNENNAIAKLDMAKGE
                     ITTHGTESYAAYANGTVVKAGDTLDYTNASVTLTDVDITTHGDNAHAIAARQGTVSFN
                     QGEIYTTGPDAAIAKIYNGGTVTLKNTSAVAHQGSGIVLESSINGQEATVDILSGSSL
                     RSANEILYHKDETSNVTITDSEVSSAADVFINNIKGHLTVDATNSKITGSANISTDDN
                     THTYLSLSDNSTWDIKADSTVSNLTVDNSTVYISRADGRDVEPTRLTITENYVGNNGV
                     LHLRTELDDDNSATDKVVINGNTSGTTRVKVTNAGGSGAYTLNGIEIISVEGESNGEF
                     IKDSRIFAGAYEYSLTRGNTEATNKNWYLTNFQATSGGETNSGGSSAPTVAPTPVLRP
                     EAGSYVANLAAANTLFVMRLNDRAGETRYIDPVTEQERSSRLWLRQIGGHNAWRDSNG
                     QLRTTSHRYVSQLGGDLLTGGFTDSDSWRLGVMAGYARDYNLTHSSVSDYRSKGSVRG
                     YSAGLYATWFADDISKKGAYIDSWAQYSWFKNSVKGDELAYESYSAKGATVSLEAGYG
                     FALNKSFGLEAAKYTWIFQPQAQAIWMGVDHNAHTEANGSRIENDANNNIQTRLGFRT
                     FIRTQEKNSGPHGDDFEPFVEMNWIHNSKDFAVSMNGVKVEQDGVSNLGEIKLGVNGN
                     LNPAASVWGNVGVQLGDNGYNDTAVMVGLKYKF"
     gene            complement(6970..7179)
                     /locus_tag="OO357_003501"
     CDS             complement(6970..7179)
                     /locus_tag="OO357_003501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001297677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888321.1"
                     /translation="MKLKKLPGFSLGLIALAVGNAYAEQFIQHDEYKHAVAIKDGKVK
                     DIDNALVITTGNGSYGYPLQERVVS"
     gene            complement(7930..9453)
                     /locus_tag="OO357_003502"
     CDS             complement(7930..9453)
                     /locus_tag="OO357_003502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707066.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HCR5888322.1"
                     /translation="MRNTLIPILVAICLFITGVAILNIQLWYSAKAEYLAGARYAANN
                     INHILEEASQATQTAVNIAGKECDLEEQYQLGTEAALKPHLRTIIILKQGIVWCTSLP
                     GNRVLLSRIPVVPDSNLLLAPAIDTVNRLPILLYQSQFADTRILVTISDQHIRGALNV
                     PLKGVRYVLRVADDIIGPTADVMTLNGHYPYTEKVHSTKYHFTIIFNPPPLFSFYRLI
                     DKGFGILIFILLIASAAAFLLDRYFNKSATPEEILRRAINNGEIVPFYQPVVNGREGT
                     LRGVEVLARWKQPHGGYISPAAFIPLAEKSGLIVPLTQSLMNQVARQMNAIASKLPEG
                     FHIGINFSASHIISPTFVDECLNFRDSFTRRDLNLVLEVTEREPLNVDESLVQRLNIL
                     HENGFVIALDDFGTGYSGLSYLHDLHIDYIKIDHSFVGRVNADPESTRILDCVLDLAR
                     KLSISIVAEGVETKEQLGYLNQNNITFQQGYYFYKPVTYIDLVKIILSKPKVKVVVE"
BASE COUNT         2660 a         2203 c         2076 g         2544 t
ORIGIN      
        1 ccgagatatt tccttgttaa ctctggggaa atgccatctg agttcacaca aatatacggg
       61 tcgcaaacaa cgccgcacgt cggagattga agtccgccag cgtcatgtcc agacgccatt
      121 gatgagaaca tcatccccac acctgacaat ccgatataaa taatacgcat catattcata
      181 gcacatcctg ataatccaac cgtacaacac ttatgctcat ttcgtcatca atccgtaatc
      241 aaaaccgctg acctggacga gttacgatga cctgacagaa gtatcagacg tcttctgcgg
      301 tttctatgat agagcaattc tgtggcggat attaagataa cgggtatctg ttctttaatg
      361 atgctgcaaa aaatggaaag ttaagaaagc ctggcgtggt gaaaaaatac acttgcctca
      421 atactggata tttaaacagg tctggagatg ctgtcacgat tagggtgcgg aaaaaggaag
      481 cgtaaataag aagaatcaaa agtacgacgg caatgggttg attgacaagg gtatttttta
      541 agctatgaat cagcgccatt tatcacagaa tagactttta ctctgaataa atgggagggt
      601 gacttgcctc aatataatcc agactataac atgccttata gtcttcggaa catcatcgcg
      661 cgctggcgat gattaatagc taattgagta aggccaggat gtcaaacact ccaatcgagc
      721 ttaaaggcag tagcttcact ttatctgtgg ttcatctgca tgaggcagaa cctaaggtta
      781 tccatcaggc gctgaaagac aaaatcgctc aggcccccac atttttaaaa catgcccccg
      841 ttgtactcaa cgtcagtgca ctggaagacc cggtaaactg gtcagcgatg cataaggcgg
      901 tttcggcaac cggtttgcgg gttattggcg taagtggctg caaagatgcg caacttaaag
      961 ccgaaattga aaaaatgggg ctgcctatcc tgacggaagg aaaagaaaaa gcgccacgtc
     1021 cagctcccgc accgcaggct ccagcgcaaa atacaatgcc ggtcacaaaa acgcgtttaa
     1081 tagatacccc ggtgcgttcc ggtcagcgta tttatgctcc acaatgtgat ctgattgtta
     1141 caagccacgt tagcgctggg gccgaattga ttgccgatgg gaacattcat gtctatggca
     1201 tgatgcgcgg tcgtgcgctg gcaggggcaa gtggtgaccg ggaaacgcaa atattttgta
     1261 cgaacctgat ggcggaattg gtgtccatcg caggtgaata ctggctgagt gatcaaatcc
     1321 cagcagaatt ttatggcaaa gcggcgcgac tgcagttagt cgaaaacgct ttgaccgttc
     1381 aaccgttaaa ttgatccctt tttaacaagg aatttctatg gcacgcatta ttgttgttac
     1441 ttcgggtaaa gggggtgttg gtaagacaac ctccagcgcg gccatcgcca ctggtttggc
     1501 ccagaaggga aagaaaactg tcgtgataga ttttgatatc ggcctgcgta atctcgacct
     1561 gattatgggt tgcgaacgcc gggtcgttta cgatttcgtc aacgtcattc agggcgatgc
     1621 aacgctaaat caggcgttaa ttaaagataa gcgtactgaa aatctctata ttcttccggc
     1681 atcgcaaaca cgcgataaag atgccctcac ccgtgaaggg gtcgccaaag ttcttgatga
     1741 tctgaaagcg atggattttg aatttatcgt ttgtgactcc ccggcaggga ttgaaaccgg
     1801 tgcgttaatg gcactctatt ttgcagacga agccattatt accaccaacc cggaagtctc
     1861 ctcagtacgc gactctgacc gtattttagg cattctggcg tcaaaatcac gccgcgcaga
     1921 aaatggcgaa gagcctatta aagagcacct gctgttaacg cgctataacc caggccgcgt
     1981 aagcagaggt gacatgctga gcatggaaga tgtgctggag atcctgcgca tcaaactcgt
     2041 cggcgtgatc ccagaggatc aatcagtatt gcgcgcctct aaccagggtg aaccggtcat
     2101 tctcgacatt aacgccgatg cgggtaaagc ctacgcagat accgtagaac gtctgttggg
     2161 agaagaacgt cctttccgct tcattgaaga agagaagaaa ggcttcctca aacgcttgtt
     2221 cggaggataa gttatggcat tactcgattt ctttctctcg cggaagaaga acacagccaa
     2281 cattgcaaaa gaacggctgc agattattgt tgctgaacgc cgtcgcagcg atgcagaacc
     2341 gcattatctg ccgcagttgc gtaaagatat tcttgaggtc atttgtaaat acgtacaaat
     2401 tgatcctgag atggtaaccg tacagcttga gcaaaaagat ggcgatattt ctattcttga
     2461 gctgaacgtg accttaccgg aagcagaaga gctgaaataa gcccgctgta aaaacgcatt
     2521 tatcttcaag gcagagtcat ttctgccttg agtttttcat ccctctcatc cacgttgtgg
     2581 taaagcggcg agtattcttg ctgatactcc tcattgctat ttccacctcc ccctttacac
     2641 cttaaggctg ttcccacgca cagaacaaaa tgtttatgcc tggttgagta aataaccatg
     2701 ttattagtgt gtatatactg ggagatgata accccgcaat ttcattattc attgttctcc
     2761 cataggatga ggtttcccgc cgtaactggc gggctttttt tgccccagaa ttttctcttt
     2821 caacatcctg taaacgaaaa ctgcgccgaa gcgcagttta atcgttgatg gtcagaacga
     2881 ccagttcaca ccagccaccg cgttccacgg ggactccaca ccggcaccat ggctataccc
     2941 caccccgaaa tgcccgctta acgtactgct gaatgaggct ttaatacctg cctggtatat
     3001 tccacgtctg cccgacaaat cattgacaaa attaccgtca ctattcactt tcacccgatt
     3061 atcatcgaca aattctttgc gcacagccgc cttcaggcag ggttcaattt ccataccgtt
     3121 ccccagacgc atgttgtaac tcagcgttgc acccagttca cgatatatac tgcgggtatc
     3181 gactgatttc gattccatgc cattggataa atgatagtag cgtctaagtg acctaagctc
     3241 tgctgcaaca tcagcccgca ggcaccagac caggggattc atcctgaaga gacagcgcag
     3301 gtgtattgtg ttcaccgctc accaaagaca tcatgatgaa atgatgatat cccgcataag
     3361 aaagaggcat tttttacacg cagtgcgctg aagtgtagtt ggataaaaaa tcactctttt
     3421 cagataatac tgtcgtacgc ttatctttag acaatgaggc cgtcggaaaa ataaaatgac
     3481 taatgtaatc gttgctgatg aatatcttct gctcacataa aaatcacaca ataatattga
     3541 taaatcagat tatcttacct ttacaacatt gcgttaacag tcaatgacac ttcttgaata
     3601 aaaatattgt caatcaatga gttattcctc atttaagatt attctcattc acgtaaaaaa
     3661 cataattcct atcatcacag ccacgctcat tttgaattat ctgaataata gcgagcgttc
     3721 tttagttaag cagatcttaa ttgaagacga aattattgtg tgtgcaactt acttaatacc
     3781 ggacatctaa gttgaagaaa aaaatacttg ccttcgggct tatctcagca ttgttctgct
     3841 ccacgccagc gatagcggat atgaaccgca ccacgaaagg tgcattgcta ggcgcaggcg
     3901 ttggtttact tactggcaat ggtgttaatg gcgtactgaa aggtgcagct gttggcgctg
     3961 gtgttagtgc agtaacagaa aaaggccgcg acggtaaaaa tgcgcgtaaa ggtgcaaaag
     4021 tgggtgccgc cgttggtgct gtcactggcg ttctgaccgg caatggcctc gaaggggcga
     4081 ttaaaggtgc cgtgattggc ggtacgggtg gtgcaattct gggtaaaatg aaataaggaa
     4141 tgatgatgaa aaaatttgct ttactggcgg gcctgtttgt tttcgcacca atgacctggg
     4201 cgcaggacta caatattaaa aacggtttgc cgtcagaaac ctatattact tgcgcagaag
     4261 ccaacgaaat ggcaaaaact gacagcgcac aggttgcaga aattgttgcg gtaatgggta
     4321 atgccagcgt tgccagccgt gatttaaaaa ttgagcaatc tccggaactg agcgccaaag
     4381 tggtagaaaa actgaatcag gtttgcgcta aagacccgca aatgctgttg atcacggcaa
     4441 ttgatgacac catgcgtgct atcggcaaaa aataatattt catatgtacc gcgtcgatga
     4501 atacgacgcg gttttttagc taacggcggg atcagaactt atatttcagg cccaccatca
     4561 ctgcggtgtc attgtagcca ttatcaccca gctgcacgcc cacattcccc cagacgctgg
     4621 ccgctggatt caggttgcca tttacgccaa gtttaatttc ccccaaatta ctcaccccat
     4681 cttgttcgac tttcacaccg ttcattgaga cagcaaaatc tttactgtta tggatccagt
     4741 tcatttcaac aaaaggttca aagtcgtcac cgtgcggacc gctgtttttc tcctgagtac
     4801 gaataaaggt gcggaagccg agtcgggtct ggatgttgtt atttgcgtca ttctcaatac
     4861 gtgagccatt ggcttccgtg tgcgcattat gatcgacgcc catccagata gcctgtgcct
     4921 gtggctggaa gatccacgta tatttcgccg cttccagacc aaaggattta ttcagggcaa
     4981 aaccgtaacc cgcttccagc gagacggttg cacctttcgc gctataggat tcataggcta
     5041 attcatcccc tttcacggag tttttaaacc agctatattg cgcccaggag tcaatgtatg
     5101 cgcctttttt actgatgtca tcggcaaacc aagtggcata cagccctgcg ctatagcctc
     5161 tgacactccc tttcgaacga taatccgaca cgctggaatg agttaagttg tagtcgcggg
     5221 cataaccagc catcactccc aaacgccaac tgtcactatc ggtaaaacca ccggttaaca
     5281 gatcgccccc taactgcgag acgtagcgat gcgaggtcgt tctcaactgt ccgttgctgt
     5341 cacgccaggc attatgcccg ccaatttgac gtagccaaag tcggcttgaa cgctcctgtt
     5401 cagttacagg atcgatgtag cgcgtttcac ccgcacggtc gttcagacgc ataacaaaaa
     5461 gagtgttagc ggctgccagg ttggcgacgt aacttccagc ttcggggcgc aggacggggg
     5521 taggcgcaac agtaggcgct gaacttcctc cggagtttgt ttcaccgccg ctcgttgcct
     5581 ggaagttagt cagataccag tttttattgg tcgcttcggt attacctcgg gttaatgaat
     5641 attcgtaggc accggcgaaa atcctcgaat ccttaataaa ttccccattt gattccccct
     5701 caacgctgat aatctctatc ccatttaacg tgtaagctcc gctgcctcct gcattggtaa
     5761 ctttaactcg ggttgttcca gaggtatttc cattaataac gactttatcc gtagctgaat
     5821 tatcatcatc caattcagtt ctgagatgca atacgccatt attaccaacg taattttcag
     5881 ttattgttaa tcttgttggt tcgacatccc gtccatctgc ccgggaaata tagaccgtac
     5941 tattatcaac cgtgaggttg ctcaccgttg agtcagcttt aatatcccag gtactattat
     6001 ctgaaagcga cagataggta tgagtgttat catctgttga aatattagct gaacccgtta
     6061 tttttgaatt agttgcatcg acggtcaaat gacctttaat attattgata aaaacatctg
     6121 cagccgatga tacctcacta tccgtaatgg tcacgttact cgtctcatct ttatggtaga
     6181 ggatttcatt tgctgaccgt agtgagctac cagataaaat atctaccgtt gcttcctgac
     6241 catttatgga agactccagc acaattccag acccctgatg cgcgactgca gatgtatttt
     6301 tcagcgtcac cgtgccgcca ttataaattt tagcaatcgc ggcgtcagga cccgttgtat
     6361 aaatttctcc ctggttaaat gaaactgtcc cctggcgggc agcgatggca tgagcattgt
     6421 ccccatgcgt ggtgatatca acatcggtta atgtaacgct ggcattggta taatcaagcg
     6481 tgtcaccagc cttgacgaca gtgccattag cgtacgcggc ataactttct gttccgtggg
     6541 ttgtgatctc cccttttgcc atatccagtt ttgctatggc attattttca ttacttcctg
     6601 tatcggcagt actttcttca tcaagagaag atacacacgc acacgcgctt ccgggagatg
     6661 cacacgcaag aacgccgact gcgtgattac ccgcagtaac aataaatgta tcatctccgt
     6721 cagaaaaaga gggggtaacc gaaccgtcag agttgactct tattatggtg tcagtattca
     6781 ggtttccact cgtttgactt agcaaaccaa ttgagtaatc ccccgttgtg acaatcgtgt
     6841 ttttaccctg caaaataaca gcaccgccaa attcacttac gattgcgcta gctgttaatg
     6901 ctgggcttag aggactattg tcagaaggat ataaaccgcc cgttgttaca atgacgccat
     6961 tgttaatggt taacttacta ccctctcctg caacggatac ccgtaacttc cattacccgt
     7021 tgttataact agcgcattat caatatcttt tacttttcca tcttttattg ccacagcatg
     7081 tttatattca tcgtgctgaa taaactgttc agcatatgca ttacccaccg ccagagcaat
     7141 aagtcccaaa ctaaacccgg ggagtttttt cagtttcata gattaactat ccattcattg
     7201 gattaacaaa cattcctggt caaataacct atgagctcag attaccaaaa cggagggtaa
     7261 agacaaaaac aaaagcacaa aaaatttaaa tagataaaca gcgacaatat ataaatgata
     7321 aataagtcgg cattatcatt tttcacctta ttttcatgac attgatcact ttggggtgac
     7381 tattttgttt aacaaataac tcttcgacat caaaattatt aatagttgcc atggtaatta
     7441 tttcggtgat atataaaaac aactgtgata acaccaaggt tgacaactat ttgcgcaata
     7501 attcctttcc ttcaatgatc caggcgttca ctgccagcaa cccattcagc cgcatactga
     7561 cagtgagaaa ctgaaagatt attgctgatc cctgtggcta acgagtcttt ctcttaaatc
     7621 gattaaccag attgatcgcc agccaaacaa gggcattaag caaatttatc gtcacaagca
     7681 cttctcccag cccggtactg ggttttgcaa taaggatgac caggagggtg atcagcataa
     7741 atgcaatgga aaagatgata tagagagtga aataatccag attattacta ttattcatgc
     7801 tactaatggc aatcctgtca aaactttcaa acaattatag caaacaaaat aatgaacaat
     7861 agtaacaaga ggcaacgagc taattagctg tctataatta caccgcacta tggccttact
     7921 gatcaatttt cactcaacca caaccttcac cttcggttta gaaaggataa ttttgaccag
     7981 gtcgatgtat gtaacaggtt tatagaaata ataaccctgc tgaaatgtga tattattttg
     8041 gttcagatag ccaagttgtt ctttcgtttc gacaccttca gcgacgatac tgatcgaaag
     8101 tttacgcgcc agatccaata cacaatccag aattcgggtt gattctgggt ctgcgtttac
     8161 gcggccaacg aaactgtgat caattttgat ataatcaata tgcaggtcat gaagataaga
     8221 aagccctgag tagccagtac cgaaatcatc cagcgcgatg acaaaaccat tttcatgcag
     8281 aatgttcaac cgctgaacca gactttcatc aacattcaat ggctcacgct cggtgacttc
     8341 cagaacaagg tttaaatcgc ggcgggtaaa actgtcacgg aaatttaaac actcgtcgac
     8401 aaacgtcggc gaaataatat gcgaagcgct aaaattaatt ccaatatgaa aaccttccgg
     8461 cagtttactc gcgatagcgt tcatctgtct ggcaacctga ttcatcaggc tttgtgtaag
     8521 cggaacgatt aatccggatt tttcagcaag tggaataaat gccgcgggtg aaatatatcc
     8581 accgtgaggt tgtttccagc gggctaacac ctcaactccc cgcaatgtcc cttcccgacc
     8641 atttaccaca ggttggtaaa aagggacgat ctccccatta tttattgccc gtcgcaggat
     8701 ctcttcaggc gttgcgcttt tattgaaata tctatcaagc aggaaggcag cggcgctggc
     8761 gattaacaga ataaatatta aaatcccaaa gcctttatcg ataagtctgt agaagctaaa
     8821 gagtggcggc gggttaaaga taatagtgaa atgatatttt gtggagtgaa ccttctcggt
     8881 atagggataa tgtccattaa gcgtcatcac atcagccgtt ggtccaataa tgtcatccgc
     8941 cacgcgtaat acatacctta cccctttcaa gggtacatta agcgccccgc gaatatgttg
     9001 atcacttatc gtaaccaaaa tgcgcgtatc tgcaaattgg ctctgataga gcaggatagg
     9061 taatctatta acggtgtcga ttgccggagc caacagtaaa ttactgtccg ggacaacagg
     9121 aatacgagac agcaggaccc gattcccagg cagggatgta caccagacta ttccctgttt
     9181 gagaatgatg attgtgcgca ggtgaggttt cagagctgct tcagtgccaa gctgatattg
     9241 ctcctcgagg tcgcattcct tcccggcaat gttaaccgct gtttgagtcg cctgtgacgc
     9301 ttcttcaagt atatgattga tattgttggc ggcatatctt gctcccgcca ggtactctgc
     9361 ttttgcggaa taccagagct ggatgttcaa aatagcgaca ccggtaataa acaagcatat
     9421 cgcaacgagg atgggtatga gtgtattgcg catttgcttg tcccggccaa agggtttaat
     9481 aca
//
LOCUS       DAJTRC010000127         9440 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.127, whole genome shotgun sequence.
ACCESSION   DAJTRC010000127 DAJTRC010000000
VERSION     DAJTRC010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9440)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9440)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9440
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.127"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            39..1154
                     /locus_tag="OO357_003503"
     CDS             39..1154
                     /locus_tag="OO357_003503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001521807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888323.1"
                     /translation="MFNHTTKTADNIEHNLQLSFLVGSRPSITNKYSAKDVGPDILAL
                     PVNVYHADFKIKINNAVEKASSIVLCAVQKNLHAARKVLSGIIGNVPKNNKPKTIIFD
                     LRFLSAIKQKVFLGSEKPLFVKHTAMMREACKELPQSVEYVPLACEAHSHRSVALAKT
                     VAAPIMATGVGLALMYATSSGPYYAQSLSGSPDIEMIKNRMAELFNQSDISVRNKYSP
                     AFDKWNELVDKLNHERNTVPFACLTTILATAINETLEGDTNVAVVMGCKSAKDRTISI
                     VLGNSMLQTLLEKRLADSGEIENLFDQQGYFSCDSLTPEELMMLKDLFDIRVLHVSNK
                     FNVGLQGNINTDVLQDSFFKNVDFIHEYNSYTVGMGV"
     gene            1833..2051
                     /locus_tag="OO357_003504"
     CDS             1833..2051
                     /locus_tag="OO357_003504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312842.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HCR5888324.1"
                     /translation="MAMNTVFLHLSEEAIKRLNKLRGWRKVSRSAILREAVEQYLERQ
                     QFPVRKAKGGRQKGEVVGVDDQCKEHKE"
     gene            2269..2511
                     /locus_tag="OO357_003505"
     CDS             2269..2511
                     /locus_tag="OO357_003505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709694.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888325.1"
                     /translation="MNSLAGIDMGRILLDLSNEVIKQLDDLEVQRNLPRADLLREAVD
                     QYLINQSQTARTSVPGIWQGCEEDGVEYQRKLREEW"
     gene            complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="OO357_003506"
     CDS             complement(2841..3770)
                     /gene="fdhE"
                     /locus_tag="OO357_003506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory protein FdhE"
                     /protein_id="HCR5888326.1"
                     /translation="MSIRIIPQDELGSSEKRTADMIPPLLFPRLKNLYNRRAERLREL
                     AENNPLGDYLRFAALIAHAQKVVLYDHPLEMDLTARIKEASAQGKPPLDIHVLPRDKH
                     WQKLLMALIAELKPEMSGPALAVIENLEKASTQELEDMASALFASDFSSVSSDKAPFI
                     WAALSLYWAQMANLIPGKARAEYGEQRQYCPVCGSMPVSSMVQIGTTQGLRYLHCNLC
                     ETEWHVVRVKCSNCEQSGKLHYWSLDDEQAAIKAESCDDCGTYLKILYQEKEPKVEAV
                     ADDLASLVLDARMEQEGYARSSINPFLFPGEGE"
     gene            complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="OO357_003507"
     CDS             complement(3767..4402)
                     /gene="fdoI"
                     /locus_tag="OO357_003507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase cytochrome b556 subunit"
                     /protein_id="HCR5888327.1"
                     /translation="MKRRDTIVRYTAPERINHWITAFCFILAAVSGLGFLFPSFNWLM
                     QIMGTPQLARILHPFVGVVMFASFIIMFFRYWHQNLINRDDIFWAKNIRKIVVNEEVG
                     DTGRYNFGQKCVFWAAIIFLVLLLVSGVIIWRPYFAPAFSIPVIRFALMLHSFAAVAL
                     IVVIMVHIYAALWVKGTITAMVEGWVTSAWAKKHHPRWYREVRKTTEKKAE"
     gene            complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="OO357_003508"
     CDS             complement(4399..5301)
                     /gene="fdoH"
                     /locus_tag="OO357_003508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase O subunit beta"
                     /protein_id="HCR5888328.1"
                     /translation="MAYQSQDIIRRSATNGLTPAPQARDFQEEVAKLIDVTTCIGCKA
                     CQVACSEWNDIRDTVGNNIGVYDNPNDLSAKSWTVMRFSEVEQNDKLEWLIRKDGCMH
                     CSDPGCLKACPAEGAIIQYANGIVDFQSEQCIGCGYCIAGCPFDIPRLNPEDNRVYKC
                     TLCVDRVVVGQEPACVKTCPTGAIHFGTKESMKTLASERVAELKTRGYDNAGLYDPAG
                     VGGTHVMYVLHHADKPNLYHGLPENPEISETVKFWKGIWKPLAAVGFAATFAASIFHY
                     VGVGPNRADEEENNLHEEKDEERK"
     gene            complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="OO357_003509"
     CDS             complement(5314..8364)
                     /gene="fdnG"
                     /locus_tag="OO357_003509"
                     /EC_number="1.17.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(7777..7779),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase-N subunit alpha"
                     /protein_id="HCR5888329.1"
                     /translation="MQVSRRQFFKICAGGMAGTTAAALGFAPSVALAETRQYKLLRTR
                     ETRNTCTYCSVGCGLLMYSLGDGAKNAKASIFHIEGDPDHPVNRGALCPKGAGLVDFI
                     HSESRLKFPEYRAPGSDKWQQISWEEAFDRIAKLMKEDRDANYIAQNAEGVTVNRWLS
                     TGMLCASASSNETGYLTQKFSRALGMLAVDNQARVUHGPTVASLAPTFGRGAMTNHWV
                     DIKNANLVVVMGGNAAEAHPVGFRWAMEAKIHNGAKLIVIDPRFTRTAAVADYYAPIR
                     SGTDIAFLSGVLLYLLNNEKFNHEYTEAYTNASLIVREDYGFEDGLFTGYDAEKRKYD
                     KSSWTYELDENGFAKRDTTLQHPRCVWNLLKQHVSRYTPDVVENICGTPKDAFLKVCE
                     YIAETSAHDKTASFLYALGWTQHSVGAQNIRTMAMIQLLLGNMGMAGGGVNALRGHSN
                     IQGLTDLGLLSQSLPGYMTLPSEKQTDLQTYLTANTPKPLLEGQVNYWGNYPKFFVSM
                     MKAFFGDKATAENSWGFDWLPKWDKGYDVLQYFEMMKEGKVNGYICQGFNPVASFPNK
                     NKVIGCLSKLKFLVTIDPLNTETSNFWQNHGELNEVDSSKIQTEVFRLPSTCFAEENG
                     SIVNSGRWLQWHWKGADAPGIALTDGEILSGIFLRLRKMYAEQGGANPDQVLNMTWNY
                     AIPHEPKSEEVAMESNGKALADITDPATGAVIVKKGQQLSSFAQLRDDGTTSCGCWIF
                     AGSWTPEGNQMARRDNADPSGLGNTLGWAWAWPLNRRILYNRASADPQGNPWDPKRQL
                     LKWDGTKWTGWDIPDYSAAPPGSGVGPFIMQQEGMGRLFALDKMAEGPFPEHYEPFET
                     PLGTNPLHPNVISNPAARIFKDDAEALGKADKFPYVGTTYRLTEHFHYWTKHALLNAI
                     LQPEQFVEIGESLANKLGIAQGDTVKVSSNRGYIKAKAVVTKRIRTLKANGKDIDTIG
                     IPIHWGYEGVAKKGFIANTLTPFVGDANTQTPEFKSFLVNVEKV"
     gene            8558..9391
                     /gene="fdhD"
                     /locus_tag="OO357_003510"
     CDS             8558..9391
                     /gene="fdhD"
                     /locus_tag="OO357_003510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HCR5888330.1"
                     /translation="MKKTQRKEIENVTNITGVRQIELWRRDDLQHPRLDEVAEEVPVA
                     LVYNGISHVVMMASPKDLEYFALGFSLSEGIIESPRDIFGMDVVPSCNGLEVQIELSS
                     RRFMGLKERRRALAGRTGCGVCGVEQLNDIGKPVQPLPFTQTFDLNKLDDALRHLNDF
                     QPVGRLTGCTHAAAWMLPSGELVGGHEDVGRHVALDKLLGRRSQEGESWQQGAVLVSS
                     RASYEMVQKSAMCGVEILFAVSAATTLAVEVAERCNLTLVGFCKPGRATVYTHPQRLS
                     N"
BASE COUNT         2311 a         2315 c         2446 g         2368 t
ORIGIN      
        1 ttgaggatat gtgtgaaaga atgaaaggtg gcatctctat gtttaaccac accacaaaaa
       61 cagctgataa tatagagcat aatttgcagc taagttttct cgtgggttca cgtccgtcga
      121 tcaccaataa atactcagca aaagatgtag gtccagatat acttgcattg cccgtcaacg
      181 tatatcatgc ggattttaaa attaaaatca ataatgcagt ggagaaggct tcatcaattg
      241 tgttatgtgc tgtgcaaaaa aatctccatg cagcaaggaa ggttcttagc gggataatag
      301 gaaatgtgcc aaaaaataat aaaccaaaaa caattatttt tgatttgcgt tttcttagtg
      361 caattaagca gaaagttttt ttgggcagtg aaaaaccgtt gtttgtaaag cataccgcaa
      421 tgatgaggga ggcatgtaag gaattaccgc agtcagtgga atatgttcct ttagcctgtg
      481 aagcgcatag ccatcgcagc gtcgcccttg ccaaaaccgt ggctgcgccc atcatggcga
      541 caggtgtggg cttggcatta atgtatgcaa catcatcagg accgtattat gcgcaaagtc
      601 tttctggttc gccggatatc gagatgataa aaaatcgcat ggctgaattg tttaaccagt
      661 cggatatttc tgtgcgcaac aaatatagcc cggcttttga taaatggaat gaactggtag
      721 ataaattaaa tcacgaaaga aatacagttc cgtttgcgtg tttgaccacc attttagcga
      781 cggcgataaa tgaaactctt gagggggata cgaatgttgc cgttgtgatg gggtgtaaat
      841 ctgcgaaaga tcgcacgata tccattgtcc ttggcaactc gatgttgcaa acactccttg
      901 aaaaacgcct cgccgatagt ggagaaatag aaaatctttt tgatcaacag ggatatttta
      961 gttgcgattc attaacaccc gaagaattaa tgatgttaaa agaccttttt gatattcgag
     1021 ttcttcatgt ctctaacaaa ttcaatgttg gattgcaagg taatatcaac actgatgttt
     1081 tacaagattc gttttttaaa aatgtcgatt tcattcatga atataactcg tataccgtag
     1141 ggatgggagt ctgacgagta aaactcatta ggattatcag aattcggaca atgcattgca
     1201 aaaatgacat acaaagagag atgtgtgcag gtacttagcc gcgaggggca tggcgaagcc
     1261 atacagaaag taccagtatt cagaactcca gtgaaagcgg catagtggcc gcctgctcag
     1321 gagttttgct atgacccgta atcgtgctga acgtcgccac cctatgatgc gattaaaaaa
     1381 actcatcgtc gcgctgatga ttgacgtcaa tgctatcgga tgatgccggg ggtgttccgg
     1441 aaggaattcc ttgtttcccg aactgttacc aacaaaatta taacgatgag caagtaaaga
     1501 ccggttggtg gcagtactga ccggtctgtg atgaaagatt aatttcacct taaatcctgg
     1561 tgctgttagg atgctgacgt ttttcggcta caccattcag attatgtaaa taaatatccc
     1621 tgaatataat atattcatag cacccataac tcatcctggg cgataactaa accatttttg
     1681 agtaataaat gaaacaactg caacgctctt attatttctg gaaaacgcct cgcaaaaaca
     1741 aaatgacatg caaaaatccg cattttttta aatgcttttc cggcgtaatt ttcgcacata
     1801 cattaatgag taatatatgt aaggtggtgg taatggcgat gaatacggtt tttcttcatt
     1861 tatcagaaga ggcaattaaa cggctgaaca agcttcgggg ctggcgtaag gtttcacgat
     1921 ctgcaatttt acgcgaagcg gttgagcaat atctggagcg gcagcaattt ccggtgcgta
     1981 aagcaaaggg aggcaggcaa aagggggagg tagttggtgt tgatgatcag tgtaaggagc
     2041 ataaggaatg aaaaaaattt ttcattcttg aatataaaaa acagatgccc ttattctggt
     2101 attaatacaa ggctgtttta cttgaactta taataactgc aactgttaca tcgtatctgg
     2161 aaaacgcctc gcaaaaataa aaaatgatgc gtaaatgagc tttctttaaa gattcggcaa
     2221 gggtactttt ctctccatag attcaaaaat gatattggcg agatatttat gaactctctg
     2281 gcaggtatag atatgggcag aattttactc gatttatcga atgaggtgat taagcaactt
     2341 gatgatctcg aggtgcagcg taatcttcct cgtgcggacc tattaaggga agcggtagat
     2401 caatacctga taaatcaatc gcaaacagca agaaccagtg ttcctggcat ctggcaaggg
     2461 tgtgaggaag atggtgtcga atatcagcgt aagctgcgcg aggaatggta atgggaaaaa
     2521 atgatgtcaa tcagattgct gacaacgtgc gcgttgttca tgccggatgc ggcgtgaacg
     2581 ccttatccgg cctacaaagt catgcaaatt caatatattg aagtttattg gtaggcctga
     2641 taagcgcagc gcatcaggca atcatacgct tgtcatcagt gacataccca taaccttatc
     2701 gatgtgttgg agggaagaac gaggcaaaca gacaattgcg gcatgatctt gaatgggtaa
     2761 cgcggaatac gagagatggt gtcgtcacac cctatgagct gtaatccggg cgacctgcgc
     2821 cacccggaaa acgtcagaga ttactctcct tcacccggaa acaggaacgg gttaatggaa
     2881 ctacgggcgt agccttcttg ctccattcgc gcgtccagta ccagagaggc gaggtcatct
     2941 gccacggctt caactttcgg ttctttttcc tgataaagaa tcttcaggta agtgccgcag
     3001 tcatcgcagc tttcggcttt aatcgcggcc tgttcgtcat ccagcgacca gtaatgcagt
     3061 ttgccgctct gctcgcagtt gctgcatttt acgcgcacta cgtgccattc ggtttcacac
     3121 aggttgcagt gcaggtaacg cagaccctga gtggtgccaa tttgcaccat gctggacacc
     3181 ggcatagagc cacataccgg gcaatattga cgttgttcgc cgtattcagc gcgggctttg
     3241 ccggggatca gattggccat ctgcgcccag tagagcgaca gcgcagccca gataaacggc
     3301 gctttatcgc tgctgacgga cgagaaatca gaggcaaaca gcgcgctggc catatcttcc
     3361 agctcctgag tcgatgcctt ctccagattc tcaatcactg ccagcgccgg gccgctcatt
     3421 tcaggtttca gctcagcaat tagcgccatc agcagctttt gccagtgctt atcacgcggc
     3481 agaacgtgaa tatccagcgg gggcttgccc tgtgcgctgg cttctttaat gcgcgcggtc
     3541 agatccatct ccagcggatg gtcgtacagc accactttct gggcgtgggc gataagcgca
     3601 gcaaagcgca ggtaatcacc cagcggatta ttttctgcca gctcgcgcag acgctcggcg
     3661 cggcggttgt ataaattctt gagccgaggg aacaataacg gcggaatcat atccgccgta
     3721 cgtttctcgc tcgaacccag ctcatcttgc gggattatgc gaatactcat tcagctttct
     3781 tttccgttgt cttgcggacc tcacggtacc agcgcgggtg atgtttcttc gcccaggcgc
     3841 tggtaaccca tccttccacc atggcggtaa tcgtgccttt cacccaaagg gcggcgtaga
     3901 tatgcaccat gataaccaca attaacgcca ctgcggcaaa tgaatgcagc attaacgcga
     3961 atcggatcac cgggattgag aaagcaggcg caaaataagg acgccagata atcacgccgc
     4021 tcaccagcag cagaaccagg aaaataatcg ccgcccagaa aacgcatttc tgaccgaagt
     4081 tataacgccc ggtgtcacct acttcctcgt tgacgacgat cttacgaata ttcttcgccc
     4141 aaaagatatc atcccgattg attaggtttt gatgccagta acggaaaaac atgatgatga
     4201 acgaggcaaa cataaccacg ccgacaaacg ggtgcagaat tcgcgccagc tgcggtgtgc
     4261 ccatgatttg catcaaccag ttgaaggacg ggaacaaaaa gcccagcccg ctcaccgccg
     4321 ccaggatgaa gcagaaggcg gtgatccagt ggttgatacg ttccggcgcg gtgtagcgca
     4381 cgatggtgtc acgtcgtttc atttgcgctc ctcgtctttc tcttcgtgca gattattctc
     4441 ttcctcatcc gcacggttcg gaccgacacc cacgtagtgg aagatactgg ccgcaaaggt
     4501 agccgcaaag ccaacagccg cgagcggttt ccagatgcct ttccagaatt tcacggtttc
     4561 gctgatttcc gggttctccg gcaagccatg atacagattt ggcttgtcag catggtgcag
     4621 cacgtacatg acgtgtgtac caccgacgcc tgccggatcg tacagacccg cattgtcgta
     4681 accgcgggtt ttcagctcag caacgcgctc gctcgccagc gttttcatcg actctttcgt
     4741 gccgaagtga atcgcgcccg ttgggcaggt cttcacgcag gctggttctt gcccaaccac
     4801 cacgcggtca acgcacagcg tacatttgta gacgcggttg tcttccgggt tgaggcgcgg
     4861 aatgtcgaac ggacagcccg caatgcaata accgcagcca atgcactgct cggactggaa
     4921 gtcgacgata ccgttggcat actgaataat tgcaccttcc gccgggcacg ctttcaggca
     4981 gcctggatcg gaacagtgca tacagccgtc tttgcggatc agccattcca gtttgtcgtt
     5041 ctgctccact tccgagaagc gcattaccgt ccacgatttg gcgcttaaat cattggggtt
     5101 gtcgtacacc ccaatgttat tgccgacggt atcgcgaatg tcgttccact ctgaacacgc
     5161 cacctgacag gctttacagc cgatacaggt ggtaacgtcg atgagtttcg ccacttcttc
     5221 ctggaagtcc cgcgcctgag gcgcgggggt cagaccgtta gtcgcggaac gacgaatgat
     5281 atcttgagat tgataagcca tatgtcgtct ccgttacacc ttttccacat tcacaaggaa
     5341 ggacttaaac tccggcgtct gcgtgttcgc atcaccaacg aatggcgtta aagtattggc
     5401 aataaagcct tttttagcta cgccttcata gccccagtga atcggaatac cgatggtatc
     5461 gatatctttg ccgttcgctt tcagcgtgcg aatacgtttg gtcaccaccg ctttggcttt
     5521 gatatagcca cggttggagg agactttcac ggtatcgccc tgggcaatgc caagtttatt
     5581 cgccagcgac tccccgattt ccacaaactg ctctggctgc aaaatcgcgt tcaacagcgc
     5641 gtgtttggtc cagtagtgga agtgctcggt cagacgatag gtggttccga catacgggaa
     5701 cttatcggct ttacccaatg cttcggcgtc gtctttaaag atacgtgcag ctgggttcga
     5761 gataacgttt ggatgcagcg ggttagttcc cagcggcgtt tcaaacggct cgtagtgttc
     5821 cgggaacgga ccttccgcca tcttatcgag ggcaaacaga cgccccatgc cttcctgctg
     5881 catgataaac ggcccgacgc cgctacccgg aggcgctgcg ctgtagtccg gaatatccca
     5941 gccggtccac ttagtgccgt cccatttcag caactgacgc ttcggatccc acgggttacc
     6001 ctgcggatct gcggaggcgc ggttatacag aatgcggcgg ttaagcggcc atgcccatgc
     6061 ccagcccagc gtgttaccga ggccagacgg atcggcgtta tcacgacgcg ccatctggtt
     6121 gccttccggc gtccagctac cggcgaaaat ccagcagcca caggaggttg taccgtcatc
     6181 gcgcaattgg gcgaacgagc taagttgttg gcctttcttg acgataaccg caccggttgc
     6241 cggatcggta atatcggcca gcgccttacc gttgctctcc atcgctactt cttccgactt
     6301 cggctcatgc ggaatggcgt agttccaggt catgttcagc acctgatccg ggttcgcgcc
     6361 accctgttcg gcatacatct tgcgcaagcg caggaagata ccggagagga tctcaccgtc
     6421 agtcagcgca atccccgggg cgtccgcacc tttccagtgc cactgcaacc agcgacctga
     6481 gttaacgatt gaaccgttct cttctgcgaa gcaggtcgat ggcagacgga acacttcggt
     6541 ctggatcttc gacgagtcaa cttcgttcag ctcaccgtgg ttctgccaga agttagaggt
     6601 ttcagtgttc agtgggtcga tggtgacgag gaacttcagt ttcgacagac aaccgatcac
     6661 tttgtttttg ttcgggaatg aggcaacagg gttaaagccc tggcagatat agccattgac
     6721 cttgccctct ttcatcatct cgaagtattg caggacgtcg tagcctttat cccacttcgg
     6781 caaccagtca aagccccagc tattttccgc cgtcgcttta tcaccaaaga aggccttcat
     6841 catagagacg aagaatttcg ggtagttgcc ccagtagttt acctggcctt ccagcagcgg
     6901 ttttggcgtg ttggcggtaa ggtaggtttg cagatcggtc tgcttctcgc ttggcagcgt
     6961 catgtaacct ggcagactct gcgacagtag ccccaggtcc gtcagcccct gaatattgga
     7021 gtgaccgcgc agggcgttaa cgccgccgcc ggccatcccc atattgccga gcagcagctg
     7081 gatcatcgcc atcgtacgaa tgttttgcgc accaacggag tgttgcgtcc agccgagggc
     7141 atacaggaac gaggcagttt tatcgtgagc actggtttct gcgatgtatt cgcagacttt
     7201 caggaacgcg tcttttggcg taccacagat gttttcaacc acatctggcg tgtagcggga
     7261 aacgtgctgt ttcagcaagt tccacacgca gcgcggatgt tgcagcgtgg tatcgcgttt
     7321 ggcgaagccg ttttcgtcca gttcataagt ccaggaggat ttatcgtact tgcgtttttc
     7381 cgcgtcgtag ccggtgaaca ggccatcttc aaagccgtaa tcctcacgca cgatcaggct
     7441 ggcgttggta taggcttcgg tgtattcgtg gttgaatttt tcattgttca gcaggtacag
     7501 caatacgcct gacaggaaag caatgtcagt accggaacga ataggggcat agtagtcagc
     7561 taccgccgcc gtacgcgtaa agcgaggatc gatcacaatc agcttcgcgc cgttgtgaat
     7621 tttggcttcc atcgcccagc ggaacccgac cgggtgagct tcagcggcgt taccgcccat
     7681 caccacgacg aggttggcgt tcttgatgtc gacccagtgg ttggtcatcg caccgcgacc
     7741 aaatgttgga gcaagacttg ctaccgttgg tccgtgtcag acacgcgcct ggttgtcgac
     7801 cgcgagcata cccagcgcgc gggagaattt ttgcgttaaa tagccggttt cgttactcga
     7861 cgcggaagca cacagcatcc cggtggagag ccagcggtta acagtcacgc cttcggcgtt
     7921 ttgcgcaatg tagttagcat cgcggtcttc tttcatcagt ttggcgatgc gatcaaacgc
     7981 ctcttcccaa ctgatttgct gccatttatc agaacctggc gcacggtatt ccggaaactt
     8041 cagacggctt tcggagtgga tgaaatccac caggccagcg cctttcggac aaagtgcacc
     8101 gcggttgacc gggtgatccg gatcgccttc gatatggaag atagatgctt tggcgttttt
     8161 tgctccgtca ccgaggctgt acatcaacag cccacagcct acggaacaat aggtgcaggt
     8221 attacgggtt tcgcgggtgc gcagcagttt atactgccgg gtttccgcga gtgctacgct
     8281 gggtgcaaaa cccagtgccg ctgccgtggt gcctgccata ccgccagcgc agatcttaaa
     8341 gaactgcctt ctgctgacct gcatggattg ctccttgttt cgacattgtc acgtcccatt
     8401 tacattcgct tgctgcgtgt gcagggagtg ggagttattt ttctttgcgg aaggggccgc
     8461 aaaggtccag aattggctca atttccctcc atctaggaag ggtttgtaac agaataccat
     8521 aatgttggtg tgtatgttct tatctggtta agagaaagtg aaaaaaacac agcgaaaaga
     8581 aatcgaaaat gtgacaaata tcacaggtgt tcgtcaaatt gagttatggc ggcgtgatga
     8641 tttacaacac ccacggttag atgaggtcgc ggaagaagtt cccgttgcgc tggtctacaa
     8701 cggcatttcg catgtggtga tgatggcgtc gcccaaagac cttgagtact ttgcgctcgg
     8761 tttttcgctt tccgagggga taatagaaag tccgcgcgat atcttcggca tggatgtcgt
     8821 tccttcctgt aatggtcttg aagtacaaat tgagctttcc agccgccgct ttatggggtt
     8881 gaaggagcgc cgccgggcgc tggcgggacg tacgggctgt ggcgtatgcg gcgtggagca
     8941 acttaatgac atcggaaaac cggtgcagcc gctaccgttc acccagacgt ttgatctcaa
     9001 caaactggat gatgcattac gtcatctcaa cgattttcag ccagtggggc gactgactgg
     9061 ttgtactcac gccgctgcct ggatgttgcc atctggcgaa ctggtcggcg ggcatgaaga
     9121 cgtgggtcgc catgtggcgc tggacaaact gttaggccgc cggtcacaag aaggggaaag
     9181 ctggcagcaa ggtgcggtac tggtttccag tcgtgccagt tatgaaatgg tgcaaaagtc
     9241 ggcgatgtgc ggcgtagaga ttttgtttgc ggtgtctgcc gcgaccacgc ttgctgtaga
     9301 agtggctgag cgctgtaatc tgacactggt aggtttttgt aaaccgggtc gggcaacggt
     9361 ttatacccat ccgcagcgtt tgagcaatta attaaaataa gtcctgcgat atatattaaa
     9421 ttgcaggact tattcatttc
//
LOCUS       DAJTRC010000128         9416 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.128, whole genome shotgun sequence.
ACCESSION   DAJTRC010000128 DAJTRC010000000
VERSION     DAJTRC010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9416)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9416)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9416
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.128"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..613)
                     /locus_tag="OO357_003511"
     CDS             complement(<1..613)
                     /locus_tag="OO357_003511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FimD/PapC N-terminal domain-containing protein"
                     /protein_id="HCR5888331.1"
                     /translation="MSYLNLRLYQRNTQCLHIRKHRLAGFFVRLVVACAFAAQAPLSS
                     ADLYFNLRFLADDPQAVADLSRFENGQELPPGTYRVDIYLNNGYMATRDVTFNTGDSE
                     QGIVPCLTRAQLASMGLNTASVAGMNLLADDACVPLTTMVQDATAHLDVGQQRLNLTI
                     PQAFMSNRARGYIPPELWDPGINAGLLNYNFSGNSVQNRVMLPT"
     gene            complement(680..1405)
                     /gene="fimC"
                     /locus_tag="OO357_003512"
     CDS             complement(680..1405)
                     /gene="fimC"
                     /locus_tag="OO357_003512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria chaperone FimC"
                     /protein_id="HCR5888332.1"
                     /translation="MSNKNVNVRKSQEITFCLLAGILMFMAMMVAGRAEAGVALGATR
                     VIYPAGQKQVQLAVTNNDENSTYLIQSWVENADGVKDGRFIVTPPLFAMKGKKENTLR
                     ILDATNNQLPQDRESLFWMNVKAIPSMDKSKLTENMLQLAIISRIKLYYRPAKLALPP
                     DQAAEKLRFRRSANSLTLINPTPYYLTVTELNAGTRVLENALVPPMGESTVKLPSDAG
                     SNITYRTINDYGALTPKMTGVME"
     gene            complement(1442..1981)
                     /locus_tag="OO357_003513"
     CDS             complement(1442..1981)
                     /locus_tag="OO357_003513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418735.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5888333.1"
                     /translation="MKRKRLFLLASLLPMFALAGNKWNTTLPGGNMQFQGVIIAETCR
                     IEAGDKQMTVNMGQISSNRFHAAGEDSAPVPFVIHLRECSTVVSERVGVAFHGVADGK
                     NPDVLSVGEGPGIASNIGVALFDDEGNLVPINRPPANWKRLYSGSTSLHFIAKYRATG
                     RRVTGGIANAQAWFSLTYQ"
     gene            complement(2046..2594)
                     /gene="fimA"
                     /locus_tag="OO357_003514"
     CDS             complement(2046..2594)
                     /gene="fimA"
                     /locus_tag="OO357_003514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial major subunit FimA"
                     /protein_id="HCR5888334.1"
                     /translation="MKIKTLAIVVLSALSLSSAAALADTTTVNGGTIHFKGEVVNAAC
                     AVDAGSVDQTVQLGQVRTASLKQAGATSSAVGFNIQLNDCDTTVATKAAVAFLGTAID
                     ATRTDVLALQSSAAGSATNVGVQILDRTGNALTLDGATFSAQTTLNNGTNTIPFQARY
                     YAIGEATPGAANADATFKVQYQ"
     gene            complement(3075..3671)
                     /gene="fimE"
                     /locus_tag="OO357_003515"
     CDS             complement(3075..3671)
                     /gene="fimE"
                     /locus_tag="OO357_003515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418733.1"
                     /note="upgraded from regulatory protein to switch DNA
                     invertase; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimE"
                     /protein_id="HCR5888335.1"
                     /translation="MSKRRYLTGKEVQAMMQAVCYGATGARDYCLILLAYRHGMRISE
                     LLDLHYQDLDLNEGRINIRRLKNGFSTVHPLRFDEREAVERWTQERANWKGADRTDAI
                     FISRRGSRLSRQQAYRIIRDAGIEAGTVTQTHPHMLRHACGYELAERGADTRLIQDYL
                     GHRNIRHTVRYTASNAARFAGLWERNNLINEKLKREEI"
     gene            complement(4149..4751)
                     /gene="fimB"
                     /locus_tag="OO357_003516"
                     /pseudo
     CDS             complement(4149..4751)
                     /gene="fimB"
                     /locus_tag="OO357_003516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313298.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbria switch DNA invertase FimB"
     gene            6205..6921
                     /gene="nanC"
                     /locus_tag="OO357_003517"
     CDS             6205..6921
                     /gene="nanC"
                     /locus_tag="OO357_003517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminic acid outer membrane channel
                     NanC"
                     /protein_id="HCR5888336.1"
                     /translation="MKKAKILSGVLLLCFSSPLISQAATLDVRGGYRSGSHAYETRLK
                     VSEGWQNGWWASMESNTWNTIHDNKKENAALNDVQVEVNYAIKLDDQWTVRPGMLTHF
                     SSNGTRYGPYVKLSWDATKDLNFGIRYRYDWKAYRQQDLSGDMSRDNVHRWDGYVTYH
                     INSDFTFAWQTTLYSKQNDYRYANHKKWATENAFVLQYHMTPDITPYIEYDYLDRQGV
                     YNGRDNLSENSYRIGVSFKL"
     gene            6941..8047
                     /gene="nanM"
                     /locus_tag="OO357_003518"
     CDS             6941..8047
                     /gene="nanM"
                     /locus_tag="OO357_003518"
                     /EC_number="5.1.3.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418730.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate epimerase"
                     /protein_id="HCR5888337.1"
                     /translation="MNKTIMALAIMMASFAANASVLPETPVPFKSGTGAIDNDTVYIG
                     LGSAGTAWYKLDTQAKDKKWTALAAFPGGPREQATSAFIDGNLYVFGGIGKNSEGLTQ
                     VFNDVHKYNPKTNSWVKLMSHAPMGMAGHVTFVHNGKAYVTGGVNQNIFNGYFEDLNE
                     AGKDSTAIDKINAHYFDKKAEDYFFNKFLLSFDPSTQQWSYAGESPWYGTAGAAVVNK
                     GDKTWLINGEAKPGLRTDAVFELDFTGNNLKWNKLDPVSSPDGVAGGFAGISNDSLIF
                     AGGAGFKGSRENYQNGKNYAHEGLKKSYSTDIHLWHNGKWDKSGELSQGRAYGVSLPW
                     NNSLLIIGGETAGGKAVTDSVLISVKDNKVTVQN"
     gene            8112..9092
                     /locus_tag="OO357_003519"
     CDS             8112..9092
                     /locus_tag="OO357_003519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709926.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialate O-acetylesterase"
                     /protein_id="HCR5888338.1"
                     /translation="MNAIISPDYYYVLTVAGQSNAMAYGEGLPLPDREDAPHSRIKQL
                     ARFAHTHPGGPPCHFNDIIPLTHCPHDVQDMQGYHHPLATNHQTQYGTVGQALHIARK
                     LLPFIPDNAGILIVPCCRGGSAFTAGSEGTYSERHGASHDACRWGTDTPLYQDLVSRT
                     RAALAKNPQNKFLGVCWMQGEFDLMTSDYASHPQHFNHMVEAFRRDLKQYHSQLNNIT
                     DAPWFCGDTTWYWKENFPHSYEAIYGNYQNNVLANIIFVDFQQQGERGLTNAPDEDPD
                     DLSTGYYGSAYRSPENWTTALRSSHFSTAARRGIISDRFVEAILQFWRER"
BASE COUNT         2755 a         2057 c         1980 g         2624 t
ORIGIN      
        1 aagttggcag cattacccga ttctgtacac tatttccgct gaaattataa ttgagcaatc
       61 cggcattaat accgggatcc cataactcag gaggaatata accacgcgcg cgattactca
      121 taaatgcctg agggatcgtc aggttcagtc gctgctgacc aacatccaga tgcgcagtag
      181 cgtcctggac cattgtggtt aatggcacac aggcatcatc cgccagcaga ttcataccgg
      241 cgacagaagc cgtattcagc cccatactgg cgagttgcgc gcgtgtcagg cagggaacaa
      301 tcccttgttc actgtcgccc gtattaaatg tgacatcacg cgttgccata taaccattat
      361 tcaaatagat atcgacgcga tacgtccctg gcggtaattc ttgcccattt tcaaaacgcg
      421 ataaatcggc cacagcctgg ggatcatccg ctaaaaagcg cagattaaaa tagaggtcgg
      481 cagatgacaa aggtgcctgt gcggcaaaag cacaggcgac aacgagtcgg acaaaaaaac
      541 cagccaaacg atgcttacga atatgcaagc attgtgtgtt tcgctggtaa agtcttaaat
      601 tcagatatga cattattcct ccggtttaaa atcacccggc agtcaattct ttttattcag
      661 gcgaaaaatt ccccctgcgt tattccatta cgcccgtcat tttgggggta agtgcgccat
      721 aatcatttat tgttcggtaa gtaatattgc ttcctgcatc agaaggcaat ttaaccgtgc
      781 tttcgcccat tggaggcacc aatgcatttt caagaacccg ggttccggca ttcaactctg
      841 ttaccgtcag gtaatagggt gtcgggttaa tcagcgtcag agaattcgcg ctacgacgaa
      901 atcttaattt ttctgcggcc tgatcgggtg gcaacgctaa tttagccggg cgatagtaca
      961 gtttaatgcg gctgataatt gcgagctgta gcatattctc agtcaatttt gatttatcca
     1021 ttgacggaat cgctttaacg ttcatccaga ataaactttc ccggtcctgt ggcaattggt
     1081 tatttgttgc atcaagaata cgtaaggtat tctctttttt tcccttcatc gcaaacagag
     1141 gaggcgtcac gataaaacga ccatccttta caccatcggc attttccacc catgattgaa
     1201 ttaaataggt actattttca tcattatttg tcacggcaag ttgcacttgt ttttgccctg
     1261 ccggataaat tacgcgagtc gcacctaagg ccactcctgc ttcagcgcgt ccggcaacca
     1321 tcattgccat gaacatcagg atacctgcca gcaagcagaa tgttatttcc tgcgatttcc
     1381 ttacattgac gtttttatta ctcactgtcc tgttcctgtt atcacattat ctactgaaca
     1441 attactgata ggttaaagag aaccaggcct gggcattggc gatgccgcca gtaacccgac
     1501 gcccggtagc acgatatttg gcgatgaaat gtagcgaagt agagcctgag taaagccgtt
     1561 tccagtttgc tggaggacga ttaatcggta cgaggtttcc ttcatcatca aacaacgcta
     1621 caccaatatt ggaggctatc cctggcccct ctcccacgga gagcacatcc ggatttttac
     1681 catccgcgac accgtgaaac gccacaccca cacgttcact caccaccgtg ctacattccc
     1741 gtaaatgaat aacaaaaggc accggtgcgc tatcttcccc tgccgcatga aaccggttac
     1801 tgctgatttg ccccatattg accgtcattt gtttatcacc ggcttcaatc cggcaagttt
     1861 ccgcaataat gacgccctga aattgcatat ttccgccggg cagcgtggta ttccatttat
     1921 ttccggccag agcaaacatt ggcagcaacg acgctaacag aaataatctc ttccttttca
     1981 tcgttatttt tatcgcacaa gggtgggcat ccctgcccgt aatgacgtcc ttgaacctgg
     2041 gtaggttatt gatactgaac cttgaaggtt gcatccgcat tagctgcacc cggggttgcc
     2101 tcgccgattg cataataacg cgcctggaac ggaatggtgt tggtaccgtt attcagggtt
     2161 gtttgtgcac taaatgtcgc accgtccagc gtcagagcat tgcctgttct gtctaggatc
     2221 tgcacgccaa cgtttgttgc actacctgca gcagaactct gcagagccag tacatcagta
     2281 cgcgtagcat caattgccgt acctaagaag gcaacagcgg cttttgtggc aacagtggta
     2341 tcgcaatcat tcagctgaat gttaaaacca acggcagagc tggttgctcc agcctgcttc
     2401 aggctagcgg tacgaacctg tcccaactga acggtttgat caacagagcc tgcatcaact
     2461 gcgcaagcgg cgttaacaac ttccccttta aagtgaatgg tcccaccatt taccgtcgta
     2521 gtatcggcca gagccgctgc ggaactgagg gacagagccg acagaacaac gattgccaga
     2581 gttttaattt tcatgctgct ttcctttaaa aaaactattt ctaaatcgac atgggcagtc
     2641 gttctgtaca ctttgttttg tcaacgagtt tgactgccaa cactgcacag ttttccccca
     2701 aaagatgaaa catttggggc cattttgact catagaggaa agcatcgcgc acaaactttt
     2761 tcagtttatt tgttggctta atattctatt attatcttta tttatagatt tttatattgc
     2821 atgaggtggt ttttggagag aagaatgagg aagatgcgtc gagccacaga aacgttagct
     2881 ttacatatag cggaggtgat gtgaaattaa tttacaagag aaataattta catatcaaat
     2941 agttagatgc tttttgtcgt tttttaatat ttttatgctt gagaaaaaat acgtaactta
     3001 tttatgatat ggacagtttg gccccaattg tcttgtgttt atttgttttt taactttatt
     3061 atcaataagt taaatcaaat ctcttctctt tttaattttt cgtttatgag attatttctt
     3121 tcccataatc cggcaaaacg agcagcatta ctggcggtat aacgcacagt atggcgaata
     3181 tttcgatgcc cgagataatc ctgaattaaa cgggtatctg caccacgctc cgccagttca
     3241 taaccgcaag catgccttaa catatgagga tgagtctgcg ttacggttcc agcttcaata
     3301 ccggcatcgc gaataatgcg ataggcctgc tggcgagaaa gccgactccc gcggcgagaa
     3361 ataaatatag cgtcggtccg gtcagcgcct ttccagttag cacgttcctg ggtccagcgt
     3421 tccacggctt cacgctcatc aaaacgtaac gggtgaacgg tagaaaatcc gttcttcagt
     3481 cggcgaatat ttattcgacc ttcattaagg tcaaggtcct gataatgcag atcaagcagt
     3541 tcactaatac gcatcccatg ccgatatgcc aacagaataa gacaataatc tctggctccc
     3601 gttgccccgt aacaaaccgc ctgcatcatg gcctgaactt ctttaccggt aagataacga
     3661 cgtttactca caatagtagt actcctaact gagatatatt tgaatgaata cctataggaa
     3721 acctcaatcg gtcaaaatta gcccaataga taaaaagata accacacacg aagttatgtg
     3781 gctatcagtc aattacatta aacatcatac aaaaataaaa tatcactgat gtgacaacat
     3841 ctattttcat tgatttgatt caatataaca atagattaca gaggattaac ttccagttac
     3901 ttaaaaaata aagaaaaaag tgtggaaaca ggagaattta agtgatgtgt aaacttatga
     3961 atgattttgt ttatttattt tgaagaacat aaatcattag attttgtatg ggtatggtta
     4021 acttaatgtt tattatgata attaagaatc acacttacaa taacatcaat taataaatgg
     4081 ttaatcattt ttgatatatc gtaagaataa tgtagttttt aacaccatcc ctggtatctc
     4141 aactatctct ataaaacagc atgacgctgt cgtcctctgg ctctatccca gatgccgtaa
     4201 aaacgccctg cattgctggc ggtataccag acagtatgac gaatattgcg atgcccaaga
     4261 taatcctaga taagtcgcgt atctattccc atattcgcca aagcaaaacc acacgaatag
     4321 cgtaacatgt gcggatgaat ctccagtgac aacccggcat taccaccaga agtcgagata
     4381 atatggtaaa actgttgccg agaaagcgga ttccccttac gtgataaaaa tacccactcg
     4441 ctctcagcat gcggataaga agtacggata ctcaaccagt tttttaaagc ctgaacttct
     4501 ttgttcaata gcgggtgcgt tgttgaaaag ccttttttta atcgatggat atatatacac
     4561 tttgctttaa gatcaatatc cgaaatcctc aatcgacaaa tttcactcgc ccggaaacca
     4621 tgaataaaac aaagcaaaat cagacaataa ttacgtgttg catgaggccc ggtatttgct
     4681 gctttaagga gtgactcgat ttcactatgg gtaaggaagt tccttttttt gttatcagcc
     4741 ttattcctca tcgttttccc ttataattac agacgcgcac tagctgtgct gggttagcaa
     4801 taatccaaca tttttatcct gattatgttt ataaaagcga acgtttgctt aataacttac
     4861 gtaagtggac cagttcttct gagcgaactt aaatggagta gcgatagtta cttataataa
     4921 ctttgccata caaacgccat gctcttgcat gctatgtacc tttatatatt tatcaatctg
     4981 atacgaatct aataataacc ctcatcatta atgcaactaa tcttatctat atatcatgtg
     5041 atatgttctg taacaagtaa aatcaacata aaaaattcag caaaatttaa tatttgttaa
     5101 actagccgtc tcaaatatga cattattagt taactgcatt tgccacaaaa tggctatttc
     5161 atggaacgca gtaatttctt agtggctatt atcatgctaa ttcttagatc cctagcgatt
     5221 attcctgcct ggttcgtcag cttaggccat gagattgtta cattcctttt tatcctggag
     5281 atatggggga acgcaggcga cgctttttca tctacatccg ttggtaaata tagctttggt
     5341 cactacgact taagccttcc tcaatcacag ctattcgccg ggcgatacgg caaacaaact
     5401 aagtgaaaca tcatcctgaa aacaccaaca tcaacaagcc tctccagatc gacatcagga
     5461 gtgaccagtt acaagccaca aaccaagaaa catatttcat gatgagaatt atgctcagcc
     5521 tgatggcgag taaaccctta ttttcaccac tctcactgag ttagcttgca aagctttctt
     5581 tgtttagatt ctaaataaat tatttataaa acgtatagtt aaaagaaaat ctttagtttt
     5641 tgaagaccac cgcaagtgtt cgtctggctt cacatggcat cttcttctta gaaaaagatc
     5701 gacatatttt gtgacgcgaa ttgcaaatct ggttttgttg tatggattgc gtgatttttg
     5761 atctggtata acaggtataa agatgcacca cgatagtcaa tgagactggg catctcgtaa
     5821 tctatggcaa acatcacttc agttctttca catcgggtga tgaaaatgca cttcagtctg
     5881 aaaggaatat gaaaatgaga tcaacagaca ctctatttta tgactctggg taaaatgggt
     5941 tgagtaagtg atataactta cgaatattca aatcaattaa aaatcagaag agattttata
     6001 ctcaggtatt taatctggat ctctgtttat ttaaataatg ttaaaagaga attttcacag
     6061 gagaccttat acaaaaaata taaaatacag ttacccgttg ccaaagacac tataagcctg
     6121 gcaaaaaaat attacacaac ataaatgcta attgtttatg cgggctttgt attgctttct
     6181 gtatcctaca aatgagtgaa atttatgaaa aaggctaaaa tactttctgg cgtattatta
     6241 ctgtgctttt cgtccccatt aatttctcag gctgcgacac tggacgtacg tggtggatat
     6301 cgtagtggaa gccacgccta tgagactcga ctcaaagtca gtgagggatg gcaaaatgga
     6361 tggtgggcaa gcatggaaag taatacctgg aataccattc atgataataa aaaggaaaat
     6421 gccgcactca atgatgttca ggttgaagtt aattacgcaa ttaaacttga tgatcaatgg
     6481 acggtgcgcc cgggaatgtt aacgcatttt agcagcaacg gcacacgcta cggaccctac
     6541 gtaaaactgt cctgggacgc gacaaaagat cttaattttg gcattcgcta tcgttacgac
     6601 tggaaagctt accgacaaca agacttatcc ggtgatatgt ctcgtgataa cgttcatcgt
     6661 tgggatggat atgtcactta ccatattaat agtgatttca ccttcgcatg gcaaacgacg
     6721 ctatacagca aacagaatga ttatcgctat gcaaaccaca agaaatgggc gacggaaaat
     6781 gcctttgttc tacaatacca tatgacgccc gatattacgc catacataga atatgactac
     6841 cttgaccgcc agggtgttta caacggcaga gataatttat cggaaaacag ttatcgcatt
     6901 ggtgtgtcat ttaaactgta gtagacagga gacagtcaca atgaataaaa ctataatggc
     6961 gcttgctatc atgatggctt catttgccgc aaacgcgtct gtattaccgg aaactcctgt
     7021 gccatttaaa agtggtaccg gagcaattga taacgacact gtctacattg gtttaggtag
     7081 cgcaggtaca gcatggtaca agctagatac acaggccaaa gataaaaaat ggactgcgtt
     7141 agctgcattc cctggtggac cgagagagca agcaacctct gcatttattg atggcaatct
     7201 gtatgtgttt ggcggcattg gcaaaaacag cgagggattg actcaggtat ttaatgacgt
     7261 acacaaatac aaccccaaaa ccaatagctg ggttaaattg atgtcgcacg cgccgatggg
     7321 catggcgggt catgtgactt ttgtacacaa cggcaaggct tatgttactg gcggtgttaa
     7381 ccagaatatc ttcaatggct attttgaaga tctcaacgag gctggaaaag attcaaccgc
     7441 tatagataaa atcaacgccc actattttga caaaaaagca gaagattatt tcttcaataa
     7501 gtttctgttg tcttttgatc cctcaacaca gcaatggagt tacgctggcg aatcgccctg
     7561 gtacggaacg gctggtgcgg cggttgtgaa taaaggtgat aaaacctggc ttattaatgg
     7621 cgaagccaaa ccaggattgc gaacggatgc cgtatttgaa cttgatttca ccggtaataa
     7681 tttaaaatgg aataagcttg atcccgtctc atcaccagat ggcgtcgctg gcggttttgc
     7741 ggggataagc aatgattctc ttatatttgc tggaggggcc ggattcaaag gttcacgaga
     7801 aaattaccag aacggtaaga actatgcgca tgaaggcctg aaaaaatcat atagcactga
     7861 tattcatctt tggcataacg ggaaatggga taaatcgggt gaattatcgc aaggtcgggc
     7921 ctacggagta tcattgccct ggaataatag tttattaatt attggcggtg aaactgcagg
     7981 cggcaaagcg gtgacggatt cagttttaat ttctgtgaag gataataaag tcacagtaca
     8041 aaactaacgc ttcagggccc cggtaaaggg gccctgctat caatttgtca tttgtattac
     8101 gcgaaaatta tatgaacgca ataatatcac ccgattatta ctatgttctt accgttgctg
     8161 gtcagtctaa tgccatggcg tatggcgaag gactgccatt accggacagg gaagatgcgc
     8221 ctcattccag aattaaacaa ttagcgagat ttgcgcatac gcatcccgga ggcccgccat
     8281 gtcactttaa cgacattatt ccactgactc actgcccaca cgatgttcag gatatgcagg
     8341 gttatcacca tcctctggca acgaatcatc aaacacagta cggcaccgtt ggccaggcac
     8401 tgcatattgc acggaaatta ctgcccttca ttcctgataa tgcagggatt cttatcgttc
     8461 cgtgttgccg tggcggatcg gcttttaccg cgggcagcga agggacatat tcagaacggc
     8521 acggagccag ccatgatgct tgtcgttggg gaacggatac tccgctgtac caggatttag
     8581 tcagcagaac gcgagccgca ctggcaaaaa atccgcagaa caaattcctc ggcgtatgct
     8641 ggatgcaagg cgaatttgac ttaatgacca gtgactacgc gtcacaccct caacacttta
     8701 atcatatggt tgaagccttt cgtagggatc taaaacaata ccattctcag cttaataata
     8761 ttactgacgc gccgtggttt tgcggcgata ccacctggta ctggaaagaa aatttcccgc
     8821 attcgtatga agctatttat ggcaattatc aaaataatgt tttagccaat attattttcg
     8881 tcgacttcca gcaacaaggt gaaagaggac tgacgaacgc gcctgatgaa gacccggacg
     8941 atttaagcac gggatattac ggttcagcgt accggtcacc ggagaactgg acgacggcac
     9001 tgcgaagcag tcatttcagc acggcagccc gtcgggggat tatttctgac agatttgtag
     9061 aagcaatttt gcagttttgg cgcgaaaggt gagcggggag tttattcatg ggggatgtcc
     9121 ttgttcgttc tcagagtaga caacaaaaaa caaatgagat caaggtaata tctcttcact
     9181 tgcgggctcg aacagcaggc aattgccaat ataataatct ggcacaaggg tgaaaaacgc
     9241 gcaccgcata aaccgttgtt attgcttaca gattagccgg atgccttaat agacatccgc
     9301 gccttttcga ttatggctcg gaaatctacg aacatctaca cagtttactg gaacgttttg
     9361 ggccacagcg ttcgcagcac cgtcctgata tgccgttctg gcgattataa gtcgac
//
LOCUS       DAJTRC010000129         9400 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.129, whole genome shotgun sequence.
ACCESSION   DAJTRC010000129 DAJTRC010000000
VERSION     DAJTRC010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9400)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9400)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9400
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.129"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(26..214)
                     /locus_tag="OO357_003520"
     CDS             complement(26..214)
                     /locus_tag="OO357_003520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014334100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter"
                     /protein_id="HCR5888339.1"
                     /translation="MPHTIKKMSLIGLILMIFTSVFGFANSPSAYYLMGYSAIPFYIF
                     SALLFFIPFALMVMTPTY"
     gene            complement(433..1410)
                     /gene="epmA"
                     /locus_tag="OO357_003521"
     CDS             complement(433..1410)
                     /gene="epmA"
                     /locus_tag="OO357_003521"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000004771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P--(R)-beta-lysine ligase"
                     /protein_id="HCR5888340.1"
                     /translation="MSETASWQPSASIPNLLKRAAIMAEIRRFFADRGVLEVETPCMS
                     QATVTDIHLVPFETRFVGPGHSQGMNLWLMTSPEYHMKRLLVAGCGPVFQLCRSFRNE
                     EMGRYHNPEFTMLEWYRPHYDMYRLMNEVDDLLQQVLDCPAAESLSYQQAFLRYLEID
                     PLSADKTQLREVAAKLDLSNVADTEEDRDTLLQLLFTFGVEPNIGKEKPTFVYHFPAS
                     QASLAQISTEDHRVAERFEVYYKGIELANGFHELTDAREQQQRFEQDNRKRAARGLPQ
                     HPIDQNLIEALKVGMPDCSGVALGVDRLVMLALGAETLAEVIAFSVDRA"
     gene            1735..3543
                     /gene="frdA"
                     /locus_tag="OO357_003522"
     CDS             1735..3543
                     /gene="frdA"
                     /locus_tag="OO357_003522"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5888341.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            3536..4270
                     /gene="frdB"
                     /locus_tag="OO357_003523"
     CDS             3536..4270
                     /gene="frdB"
                     /locus_tag="OO357_003523"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5888342.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            4281..4676
                     /gene="frdC"
                     /locus_tag="OO357_003524"
     CDS             4281..4676
                     /gene="frdC"
                     /locus_tag="OO357_003524"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5888343.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            4687..5046
                     /gene="frdD"
                     /locus_tag="OO357_003525"
     CDS             4687..5046
                     /gene="frdD"
                     /locus_tag="OO357_003525"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5888344.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            5109..6242
                     /locus_tag="OO357_003526"
     CDS             5109..6242
                     /locus_tag="OO357_003526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5888345.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            6331..6864
                     /gene="blc"
                     /locus_tag="OO357_003527"
     CDS             6331..6864
                     /gene="blc"
                     /locus_tag="OO357_003527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5888346.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="OO357_003528"
     CDS             complement(6861..7178)
                     /gene="sugE"
                     /locus_tag="OO357_003528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5888347.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="OO357_003529"
     CDS             complement(7354..7500)
                     /gene="ecnB"
                     /locus_tag="OO357_003529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5888348.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="OO357_003530"
     CDS             complement(7611..7736)
                     /gene="ecnA"
                     /locus_tag="OO357_003530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5888349.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(7788..8354)
                     /gene="efp"
                     /locus_tag="OO357_003531"
     CDS             complement(7788..8354)
                     /gene="efp"
                     /locus_tag="OO357_003531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004871142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5888350.1"
                     /translation="MATYYSNDFRAGLKIMLDGEPYAVEASEFVKPGKGQAFARVKLR
                     RLLTGTRVEKTFKSTDSAEGADVVDMNLTYLYNDGEFWHFMNNETFEQLSADAKAIGD
                     NAKWLLDQAECIVTLWNGQPISVTPPNFVELEIVDTDPGLKGDTAGTGGKPATLSTGA
                     VVKVPLFVQIGEVIKVDTRSGEYVSRVK"
     gene            8396..>9400
                     /gene="epmB"
                     /locus_tag="OO357_003532"
     CDS             8396..>9400
                     /gene="epmB"
                     /locus_tag="OO357_003532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P beta-lysylation protein EpmB"
                     /protein_id="HCR5888351.1"
                     /translation="MAHIVTLNTPSREDWLTQLADVVTDPDELLRLLNIDADEKLLAG
                     RSAKKLFALRVPRSFIDRMEKGNPDDPLLRQVLTSQDEFVVASGFSTDPLEEQHSVVP
                     GLLHKYHNRALLLVKGGCAVNCRYCFRRHFPYAENQGNKRNWQTALEYVAAHPELDEM
                     IFSGGDPLMAKEHELDWLLTQLEAIPHIKRLRIHSRLPIVIPARITEALVERFARSTL
                     QILLVNHINHANEVDETFRQAMAKLRRVGVTLLNQSVLLRGVNDNAQTLANLSNALFD
                     AGVMPYYLHVLDKVQGAAHFMVSDDEARQIMRELLTLVSGYLVPKLAREIGGEPSKTP
                     L"
BASE COUNT         2376 a         2420 c         2414 g         2190 t
ORIGIN      
        1 ggcattatct gaacataaaa cactatcagt aagttggagt cattaccatt aaggcgaatg
       61 gaataaagaa taacaatgca gaaaatatat aaaagggaat cgcactataa cccattaagt
      121 aataagccga tgggctattg gcaaatccaa atacggaagt aaagatcatc aatatgagtc
      181 ctatcagact catctttttt atcgtgtgag gcatgaaacc atccttcatt tcagcgcgtg
      241 tataacacgt gcccactgaa ctgcactgcg caacattgag caggcagcgt aaaaaccaat
      301 acagaattga tacttggatg atatcaaata tccagtctta aattgtggcc tttaacggaa
      361 aaatgaaaac atatgctgca gaatggcgct tatcacgcca ttcttcgctg ttaattcagt
      421 aatttttcag aattatgccc ggtcaacgct aaaggcgatg acttcagcca gtgtctccgc
      481 gcccagcgcc aacatcacca gacgatcaac acctaatgcc acgccggaac agtcaggcat
      541 accgactttc aaggcttcaa tcagattctg gtcaatgggg tgctgcggca aaccgcgcgc
      601 cgcgcgctta cggttatctt gttcaaagcg ttgttgctgc tcacgggcat ccgttaattc
      661 atggaaacca ttcgccagct caataccttt ataataaacc tcaaagcgtt cagcgacccg
      721 atgatcttcg gtactgattt gcgccagtga tgcctggctg gctggaaagt ggtacacaaa
      781 ggtcggtttt tctttaccaa tatttggctc tacgccaaag gtaaacagca attgtagcaa
      841 cgtgtcgcgg tcttcttcgg tatcagcaac attgctcaaa tccagtttcg ctgcgacttc
      901 ccgcagttgc gttttgtcgg cagagagcgg gtcaatttcc agataacgca agaaagcttg
      961 ttgataagaa aggctttctg ctgccgggca gtccagcacc tgttgtaaga gatcgtccac
     1021 ctcgttcatc aaccggtaca tatcatagtg cggtcgatac cactccagca tagtgaactc
     1081 agggttgtga taacgcccca tctcttcatt acggaagctg cggcacagct ggaataccgg
     1141 cccacaacca gcaaccagca ggcgtttcat atggtattcc gggctggtca ttaaccagag
     1201 attcatcccc tgcgaatgcc cggggccaac gaaacgtgtc tcaaacggga ccaaatgaat
     1261 atcggttacc gtcgcctggc tcatacaagg cgtctccacc tccagcactc cacgatcggc
     1321 aaagaaacga cggatctccg ccataatcgc cgcgcgtttt aataagttag gaatggatgc
     1381 gctcggctgc caggatgccg tttcgctcat agttaaatct ccagtttttg acaagggcac
     1441 gaagtctact cgcaacgcga cggcgagaca aattttacgc aggaatcaaa cagcggtggg
     1501 cagtgactaa aaaaagcacg atctgatggt ttagtaatta aattaatcat cttcagtgat
     1561 aatttagccc tcttgcgcac taaaaaaatc gatctcgtca aatttcagac ttatccatca
     1621 gactatactg ttgtacctat aaaggagcag tggaatagcg ttcgcagacc gtaactttca
     1681 ggcacttacc ctgaagtacg gggctgtggg ataaaaacaa tctggaggaa tgtcgtgcaa
     1741 acctttcaag ccgatcttgc cattgtaggc gccggtggcg cgggattacg tgctgcaatt
     1801 gctgccgcgc aggcaaatcc aaatgcaaaa atcgcactaa tctcaaaagt atacccgatg
     1861 cgtagccata ccgttgctgc agaagggggc tccgccgctg tcgcgcagga tcatgacagc
     1921 ttcgaatatc actttcacga tacagtagcg ggtggcgact ggttgtgtga gcaggatgtc
     1981 gtggattatt tcgtccacca ctgcccaacc gaaatgaccc aactggaact gtggggatgc
     2041 ccatggagcc gtcgcccgga tggtagcgtc aacgtacgtc gcttcggcgg catgaaaatc
     2101 gagcgcacct ggttcgccgc cgataagacc ggcttccata tgctgcacac gctgttccag
     2161 acctctctgc aattcccgca gatccagcgt tttgacgaac atttcgtgct ggatattctg
     2221 gttgatgatg gtcatgttcg cggcctggta gcaataaaca tgatggaagg cacgttggtg
     2281 cagatccgtg ctaacgcggt cgttatggct accggcggtg cgggtcgcgt ttatcgttac
     2341 aacaccaacg gcggcatcgt taccggtgac ggtatgggta tggcgctaag ccacggcgtt
     2401 ccgctgcgtg acatggaatt cgttcagtat cacccaaccg gtctgccagg ttccggtatc
     2461 ctgatgaccg aaggctgccg tggtgaaggc ggtattctgg tcaacaaaaa tggctaccgt
     2521 tatctgcaag attacggcat gggcccggaa actccgctgg gcgagccgaa aaacaaatat
     2581 atggaactgg gtccacgcga caaagtttct caggccttct ggcacgaatg gcgtaaaggc
     2641 aacaccatct ccacgccgcg tggtgatgtg gtttatctcg acctgcgtca cctcggcgag
     2701 aaaaaactgc atgaacgtct gccgttcatc tgcgaactgg cgaaagcgta cgttggcgtc
     2761 gatccggtta aagaaccgat tccggtacgt ccgactgcac actacaccat gggcggtatc
     2821 gaaaccgatc agaactgtga aacccgcatt aaaggtctgt tcgccgtggg tgaatgttcc
     2881 tctgttggtc tgcacggtgc aaaccgtctg ggctccaact ccctggcgga actggtggtc
     2941 ttcggccgtc tggccggtga acaagcgaca gagcgtgcag caactgccgg taatggcaac
     3001 gaagcggcaa ttgaagcgca ggcagctggc gttgaacaac gtctgaaaga tctagttaac
     3061 caggatggcg gcgaaaactg ggcgaagatc cgcgacgaaa tgggcctggc aatggaagaa
     3121 ggttgcggta tctaccgtac gccggaactg atgcagaaaa ccatcgacaa gctggcagag
     3181 ctgcaggaac gcttcaagcg cgtgcgcatc accgacacct ccagcgtgtt caacaccgac
     3241 ctgctctaca ccattgaact gagccacggt ctgaacgttg ctgaatgtat ggcgcactcc
     3301 gcaatggcac gtaaagagtc ccgcggcgca caccagcgtc tggacgaagg ttgcaccgag
     3361 cgtgacgacg tcaactttct caaacacacc ctcgccttcc gcgatgctga tggcacgact
     3421 cgcctggagt acagcgacgt gaagattact acgctgccgc cagctaaacg cgtttacggt
     3481 ggcgaagcgg atgcagccga taaggcggaa gcagccaata ataaggagaa ggcgaatggc
     3541 tgagatgaaa aacctgaaaa ttgaggtggt gcgctataac ccggaagtcg ataccgcacc
     3601 gcatagcgca ttctatgaag tgccttatga cgcaactacc tcattactgg atgcgctggg
     3661 ctacatcaaa gacaacctgg caccggacct gagttaccgc tggtcctgcc gtatggcgat
     3721 ttgtggttcc tgcggcatga tggttaacaa cgtgccaaaa ctggcatgta aaaccttcct
     3781 gcgtgattac accgacggta tgaaggttga agcgttagct aacttcccga ttgaacgcga
     3841 tctggtggtc gatatgaccc acttcatcga aagtctggaa gcgatcaaac cgtacatcat
     3901 cggcaactcc cgcaccgcgg atcagggtac taacatccag accccggcgc agatggcaaa
     3961 gtatcaccag ttctccggtt gcatcaactg tggtctgtgc tacgccgcgt gcccgcagtt
     4021 tggcctgaac ccagagttca tcggtccggc tgccattacg ctggcgcatc gttataacga
     4081 agatagccgc gaccacggta agaaggagcg tatggcgcag ttgaacagcc agaacggtgt
     4141 atggagctgt actttcgtgg gctattgctc cgaagtctgc ccgaaacacg tcgatccggc
     4201 tgcggccatt cagcagggca aagtagaaag ttcgaaagac tttcttatcg cgaccctgaa
     4261 accacgctaa ggagtgcaac atgacgacta aacgtaaacc gtatgtacgg ccaatgacgt
     4321 ccacctggtg gaaaaaattg ccgttttatc gcttttacat gctgcgcgaa ggcacggcgg
     4381 ttccggctgt gtggttcagc attgaactga ttttcgcgct gtttgccctg aaaaatggcc
     4441 cggaagcctg ggcgggattc gtcgactttt tacaaaaccc ggttatcgtg atcattaacc
     4501 tgatcactct gacggcagct ctgctgcaca ccaaaacctg gtttgaactg gcaccgaaag
     4561 cggccaatat cattgtaaaa gacgaaaaaa tgggaccaga gccaattatc aaaagtctct
     4621 gggcggtaac tgtggttgcc accatcgtaa tcctgtttgt tgccctgtac tggtaaggag
     4681 cctgagatga ttaatccaaa tccaaagcgt tctgacgaac cggtattctg gggcctcttc
     4741 ggggccggtg gtatgtggag cgcaattatt gcgccggtga tgatcctgct ggtgggtatt
     4801 ctgctgccac tggggctgtt tccgggtgat gcgctgagct acgagcgcgt tctggcgttc
     4861 gcgcagagct tcattggtcg cgtattcctg ttcctgatga tcgttctgcc gctgtggtgt
     4921 ggtttacacc gtatgcacca cgcgatgcac gatctgaaaa tccacgtgcc tgcgggcaaa
     4981 tgggttttct acggtctggc tgctatcctg acagttgtca cgctgattgg tgtcgttaca
     5041 atctaacgca tcgccaatgt aaatccggcc cgcctatggc gggccgtttt gtatggaaac
     5101 cagaccttat gttcaaaacg acgctctgcg ccttattaat taccgcctct tactccacat
     5161 ttgctgcccc tcaacaaatc aacgatattg tgcatcgcac aattaccccg cttatagagc
     5221 aacaaaagat ccccggtatg gcggtggcgg taatttatca gggtaaacct tattacttta
     5281 cctggggcta tgcggacatc gccaaaaagc agcccgtcac acagcaaacg ttgtttgagt
     5341 taggttcggt cagcaaaaca tttacgggcg tgcttggtgg cgacgctatt gctcgagggg
     5401 aaatcaagtt aagcgatccc gcaacaaaat actggcctga acttaccgct aaacagtgga
     5461 atgggatcac actattacat cttgcgacct acaccgctgg cggcctgcca ttgcaggtgc
     5521 cggatgaagt gaaatcctca agcgacttgc tgcgcttcta tcaaaactgg cagcctgcat
     5581 gggctccagg aacacaacgt ctgtatgcca actccagtat cggtttgttc ggcgcactgg
     5641 ctgtgaagcc gtctggtttg agttttgagc aggcgatgca aactcgtgtc ttccagccac
     5701 tcaaactcaa ccatacgtgg attaatgtac ctcccccaga agaaaagaat tacgcctggg
     5761 gatatcgcga aggtaaggca gtgcatgttt cgccaggggc gttagatgct gaagcttatg
     5821 gtgtgaagtc gaccattgaa gatatggccc gctgggtgca aagcaattta cccccccttg
     5881 atatcaatga gaaaacgctt caacaaggga tacaactggc acaatctcgc tactggcaaa
     5941 ccggcgatat gtatcagggc ctgggctggg aaatgctgga ctggccggtc aatcctgaca
     6001 gcatcattaa cggcagtgac aataaaattg cactggcagc acgcccggta aaagcgatta
     6061 agcccccaac tcctgcagta cgcgcatcat gggtacataa aacgggggcg accggcggat
     6121 ttggtagcta tgtcgcgttt attccagaaa aagagctggg tatcgtgatg ctggctaaca
     6181 aaaactatcc caatccagcg agagtcgccg ccgcctgtca gattctcaac gctctacagt
     6241 aaaatttcat cgggtccgaa ttttcggacc ttttctgcgc ttttccttgc tgtcatctac
     6301 acttagaaaa aaaccagtaa ggaaacattt atgcgcctgc tccctctcgt tgccgcagcg
     6361 acagctgcat ttctggtcgt tgcctgtagt tttcctacgc cgccgcgtgg cgtgaccgta
     6421 gtgaataatt tcgacgccaa acgttatctc ggtacctggt atgagattgc ccgttttgat
     6481 caccgttttg aacgtggact ggaaaaagtc accgcaacat acagcctgcg tgatgacggc
     6541 ggcctgaatg tcattaataa aggctataac cctgacagag gaatgtggca gcagagtgaa
     6601 gggaaagcgt actttaccgg cgcaccaact cgcgctgcgc tgaaagtgtc attctttggt
     6661 cctttctatg gcggttataa cgttattgca ctcgatcggg aataccgcca tgcgctggtt
     6721 tgcggcccgg accgcgacta cctgtggata ctctcccgca cgccaaccat ttctgacgaa
     6781 gtgaaacagg agatgctggc agtcgcgacc cgggaagggt ttgatgtcag taagtttatt
     6841 tgggtacagc agcccggtag ttagtgagtg ctgagtttca gaccaataat ccccaatacg
     6901 attaacgcca gactcgccag acgcatcggg ttagcggact caccgagcag cacaatgccg
     6961 gtgatggccg cgccgactgc gccaataccc gtccacacgg cataagccgt ccctaccggt
     7021 aacgatttca tcgcccaggc aagtagcgcc atactgacta tcatcgccgt cacggtaata
     7081 acactcggcg tcaaacggct aaagccgtgg gtatatttca ggccaacggc ccataccact
     7141 tccagcagac cagcaataac taagataatc caggacatat caggctccgg aaatttgggg
     7201 ccgtccccgg tgaaaagagg cgtttgcagg tcgtcctgca aagctgatgg ttgaacggat
     7261 attttgccag gtggcagggg aatttcaact attaccttta tgacgatcgc agaaaaaaca
     7321 aacggcacga cactgctgtc gtaccgttat tgcttattgc tgcgctttcg ttgctgcgcc
     7381 agaaatcgcg ttaccgccat cagaaatgtc ttcaccaacg ccacgcgtgg tgttgcaggc
     7441 agttaatact gttgaaagca ccagaacaga aaagatcgct gcaattgtct tcttcaccat
     7501 aacgtcttcc ttttaggtaa tgttatttat gtttgcctat agcaaattaa taatagacaa
     7561 catcgccaaa aatgatggaa atctgatgat ttttcggaag agaagaaaaa ttagctggca
     7621 gcgcgagaga tggagttacc gagatgtttg atgtcttcgc cgaaaccgca agcggtgtta
     7681 cagcccgtga gcagcgtgct ggcaagcaaa acaagaacga taaggcgttt catcatctct
     7741 gacctgaata agtgatggtg cagcctgcag gccgcaccac aaccgcatta cttcacgcga
     7801 gagacgtatt caccagagcg ggtatccact ttgatgactt cgccgatttg tacgaacagc
     7861 ggaactttaa ccacagcgcc agtagacagg gtagccggtt tgccgccagt acctgcggta
     7921 tcacctttca ggcccggatc ggtatcaacg atttccagtt caacgaagtt cggcggagta
     7981 acggagatcg gctgaccatt ccacagagtt acgatacact ctgcctgatc cagcagccat
     8041 ttagcattgt cgccaattgc ttttgcatca gcagacagct gctcgaaagt ttcgttgttc
     8101 atgaagtgcc agaactcacc gtcgttgtac aggtaagtca ggttcatatc gacaacatca
     8161 gcgccttcag cggaatcagt agatttgaag gttttttcta cgcgagtacc ggtcagcaga
     8221 cgacgcagtt taacgcgagc aaatgcctgg cctttacccg gttttacgaa ttcactcgct
     8281 tcaaccgcgt aaggttcgcc gtctaacatg attttaagac cagcacgaaa atcgttgcta
     8341 tagtacgttg ccataaggcc ctctgaaatt tgttaattgg tagctaagcc acaaaatggc
     8401 gcatattgta accctaaata ccccatccag agaagattgg ttaacgcaac ttgccgatgt
     8461 tgtgaccgat cctgatgaac ttctgcgtct tttgaatata gacgcggacg aaaaactgtt
     8521 ggccggacgc agcgccaaaa agctgtttgc cctgcgtgtg ccccgctcat ttatcgatcg
     8581 catggagaaa ggcaatccgg acgatcctct tttgcgtcag gtacttacct cgcaagatga
     8641 gtttgtcgtc gcgtccggat tctccaccga cccactggaa gaacagcaca gcgtagtgcc
     8701 tggtttgttg cataaatacc acaaccgggc gcttttgctg gtcaaaggcg gctgcgcggt
     8761 aaattgccgc tattgcttcc gtcgccactt cccctatgcc gaaaatcagg gcaacaagcg
     8821 taactggcag actgcacttg agtatgttgc tgcgcatccg gaactggacg agatgatttt
     8881 ctccggcggc gatccgctga tggcgaaaga gcacgagctg gactggttgc tcacacaact
     8941 ggaagccatc ccgcatatca aacgtctgcg gattcacagc cgtctgccga ttgtgatccc
     9001 ggcacgtatc accgaggcgc tggttgaacg ctttgcccgt tctacgctgc aaatcttgct
     9061 ggtgaatcac atcaatcatg ccaatgaggt agatgaaacg ttccgtcagg cgatggctaa
     9121 attgcgccgt gtcggtgtca ccctgcttaa ccagagcgtt ctgttacgtg gtgtgaacga
     9181 taacgcacaa acgttggcaa acctgagtaa tgcgttgttc gatgccggcg tgatgccgta
     9241 ttacctgcat gtgctagata aagtacaggg cgcggcgcat tttatggtga gtgatgacga
     9301 agcacggcag attatgcgtg agttgctgac attggtgtcg ggatatctgg tgccgaaact
     9361 ggcgcgagaa attggcggcg aacccagcaa aacgccgctg
//
LOCUS       DAJTRC010000130         9394 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.130, whole genome shotgun sequence.
ACCESSION   DAJTRC010000130 DAJTRC010000000
VERSION     DAJTRC010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9394
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.130"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            78..1196
                     /locus_tag="OO357_003533"
     CDS             78..1196
                     /locus_tag="OO357_003533"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HCR5888352.1"
                     /translation="MPLPDFHVSEPFTLGIELEMQVVNPPGYDLSQDSSMLIDAVKNK
                     ITAGEVKHDITESMLELATDVCRDINQAAGQFSAMQKVVLQAAADHHLEICGGGTPPF
                     QKWQRQEVCDNERYQRTLENFGYLIQQATVFGQHVHVGCASGDDAIYLLHGLSRFVPH
                     FIALSAASPYMQGTDTRFAPSRPNIFSAFPDNGPMPWVSNWQQFEALFRCLSYTTMID
                     SIKDLHWDIRPSPHFGTVEVRVMDTPLTLSHAVNMAGLIQATAHWLLTERPFKHQEKD
                     YLLYKFNRFQACRYGLEGVITDPHTGDRRPLTEDTLRLLEKIAPSAHKIGASSAIEAL
                     HRQVVSGLNEAQLMRDFVADGGSLIGLVKKHCEIWAGD"
     gene            1262..1510
                     /locus_tag="OO357_003534"
     CDS             1262..1510
                     /locus_tag="OO357_003534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1158 domain-containing protein"
                     /protein_id="HCR5888353.1"
                     /translation="MKHPLETLTTAAGILLMAFLSCLLLPAPALGLTLAQKLVTMFHL
                     MDLSQLYTLLFCLWFLVLGAIEYFVLRFIWRRWFSLAD"
     gene            1575..1943
                     /locus_tag="OO357_003535"
     CDS             1575..1943
                     /locus_tag="OO357_003535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HCR5888354.1"
                     /translation="MDKQSLHETAKRLALELPFVELCWPFGPEFDVFKIGGKIFMLSS
                     ELRGVPFINLKSDPQKSLLNQQIYPSIKPGYHMNKKHWISVYSGEEISEALLRDLIND
                     SWNLVVDGLAKRDQKRVRPG"
     gene            2037..2690
                     /gene="nfsB"
                     /locus_tag="OO357_003536"
     CDS             2037..2690
                     /gene="nfsB"
                     /locus_tag="OO357_003536"
                     /EC_number="1.5.1.34"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-insensitive NAD(P)H nitroreductase"
                     /protein_id="HCR5888355.1"
                     /translation="MDIISVALKRHSTKAFDASKKLTPEQAEQIKTLLQYSPSSTNSQ
                     PWHFIVASTEEGKARVAKSAAGNYVFNKRKILDASHVVVFCAKTAMDDAWLKLVVDQE
                     DADGRFATPEAKAANDKGRKFFAYMHRKDLHDDAEWMAKQVYLNVGNFLLGVAALGLD
                     AVPIEGFDAAILDAEFGLKEKGYTSLVVVPVGHHSVEDFNATLPKSRLPQNTTLTEV"
     gene            2798..4045
                     /gene="ybdG"
                     /locus_tag="OO357_003537"
     CDS             2798..4045
                     /gene="ybdG"
                     /locus_tag="OO357_003537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel YbdG"
                     /protein_id="HCR5888356.1"
                     /translation="MQDLISQVEDLAGIEIDHTTSMVMIFGIIFLTAVVVHIILHWVV
                     LRTFEKRAIASSRLWLQIITQNKLFHRLAFTLQGIIVNIQAVFWLQKGTEAADILTTC
                     AQLWIMMYALLSVFSLLDVILNLAQKFPAASQLPLKGIFQGIKLIGAILVGILMISLL
                     IGQSPAILISGLGAMAAVLMLVFKDPILGLVAGIQLSANDMLKLGDWLEMPKYGADGA
                     VIDIGLTTVKVRNWDNTITTIPTWSLVSDSFKNWSGMSASGGRRIKRSISIDVTSIRF
                     LDEDEMQRLNKAHLLKPYLTSRHQEINEWNRQQGSTESVLNLRRMTNIGTFRAYLNEY
                     LRNHPRIRKDMTLMVRQLAPGDNGLPLEIYAFTNTVVWLEYESIQADIFDHIFAIVEE
                     FGLRLHQSPTGNDIRSLAGAFKQ"
     gene            complement(4113..5489)
                     /gene="pheP"
                     /locus_tag="OO357_003538"
     CDS             complement(4113..5489)
                     /gene="pheP"
                     /locus_tag="OO357_003538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine transporter"
                     /protein_id="HCR5888357.1"
                     /translation="MKNASTVSEDTASNQEPTLHRGLHNRHIQLIALGGAIGTGLFLG
                     IGPAIQMAGPAVLLGYGVAGIIAFLIMRQLGEMVVEEPVSGSFAHFAYKYWGPFAGFL
                     SGWNYWVMFVLVGMAELTAAGIYMQYWFPDVPTWIWAAAFFIIINAVNLVNVRLYGET
                     EFWFALIKVLAIIGMIGFGLWLLFSGYGGEKASIDNLWRYGGFFATGWNGLILSLAVI
                     MFSFGGLELIGITAAEARDPEKSIPKAVNQVVYRILLFYIGSLVVLLALYPWVEVKSN
                     SSPFVMIFHNLDSNVVASALNFVILVASLSVYNSGVYSNSRMLFGLSVQGNAPKFLTR
                     VSRRGVPINSLMLSGAITSLVVLINYLLPQKAFGLLMALVVATLLLNWIMICLAHLRF
                     RAAMRRQGRETQFKALLYPFGNYLCIAFLGMILLLMCTMDDMRLSAILLPVWIVFLFV
                     AFKTLRRK"
     gene            complement(5590..8733)
                     /gene="cusA"
                     /locus_tag="OO357_003539"
     CDS             complement(5590..8733)
                     /gene="cusA"
                     /locus_tag="OO357_003539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter permease
                     subunit CusA"
                     /protein_id="HCR5888358.1"
                     /translation="MIEWIIRRSVANRFLVLMGALFLSIWGTWTIINTPVDALPDLSD
                     VQVIIKTSYPGQAPQIVENQVTYPLTTTMLSVPGAKTVRGFSQFGDSYVYVIFEDGTD
                     PYWARSRVLEYLNQVQGKLPAGVSAELGPDATGVGWIYEYALVDRSGKHDLADLRSLQ
                     DWFLKYELKTIPDVAEVASVGGVVKEYQVVIDPQRLAQYGISLAEVKSALDASNQEAG
                     GSSIELAEAEYMVRASGYLQTLDDFNHIVLKASENGVPVYLRDVAKVQIGPKMRRGIA
                     ELNGEGEVAGGVVILRSGKNAREVIAAVKDKLETLKSSLPEGVEIVTTYDRSQLIDRA
                     IDNLSGKLLEEFIVVAVVCAPFLWHVRSALVAIISLPLGLCIAFIVMPFQGLNANIMS
                     LGGIAIAVGAMVDAAIVMIENAHKQLEEWQHQHPDATLDNKTRWQVITDASVEVGPAL
                     FISLLIITLSFIPIFTLEGQEGRLFGPLAFTKTYAMAGAALLAIVVIPILMGYWIRGK
                     IPPESSNPLNRFLIRVYHPLLLKVLHWPKTTLLVAALSVLTVLWPLNKVGGEFLPQIN
                     EGDLLYMPSTLPGISAAEAASMLQKTDKLIMSVPEVARVFGKTGKAETATDSAPLEMV
                     ETTIQLKPQDQWRPGMTMDKIIEELDNTVRLPGLANLWVPPIRNRIDMLSTGIKSPIG
                     IKVSGTVLADIDAMAEQIEEVARTVPGVASALAERLEGGRYINVEINREKAARYGMTV
                     ADVQLFVTSAVGGAMVGETVEGIARYPINLRYPQSWRDSPQALRQLPILTPMKQQITL
                     ADVADVKVSTGPSMLKTENARPTSWIYIDARDRDMVSVVHDLQKAIAEKVQLKPGTSV
                     AFSGQFELLERANHKLKLMVPMTLMIIFVLLYLAFRRVGEALLIISSVPFALVGGIWL
                     LWWMGFHLSVATGTGFIALAGVAAEFGVVMLMYLRHAIEAEPSLNNPQTFSEQKLDEA
                     LYHGAVLRVRPKAMTVAVIIAGLLPILWGTGAGSEVMSRIAAPMIGGMITAPLLSLFI
                     IPAAYKLMWLHRHRVRN"
     gene            complement(8745..9263)
                     /gene="cusB"
                     /locus_tag="OO357_003540"
                     /pseudo
     CDS             complement(8745..9263)
                     /gene="cusB"
                     /locus_tag="OO357_003540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308639.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(+)/Ag(+) efflux RND transporter periplasmic
                     adaptor subunit CusB"
BASE COUNT         2282 a         2604 c         2309 g         2199 t
ORIGIN      
        1 cattccagaa cttccgcatc tcgcccagcc gctccgactt ctatactgaa tagaaaacgt
       61 caacttaaga gaaacctatg ccattacccg attttcatgt ttctgaacct tttaccctcg
      121 gtattgaact ggaaatgcag gtggttaatc cgccgggcta tgacttaagc caggactctt
      181 caatgctgat tgacgcggtt aaaaataaga tcacggccgg agaggtaaag cacgatatca
      241 ccgaaagtat gctggagctg gcgacggatg tttgccgtga tatcaaccag gctgccgggc
      301 agttttcagc gatgcagaaa gtcgtattgc aggcagccgc agaccatcat ctggaaattt
      361 gcggcggtgg cacgcccccg tttcagaaat ggcagcgtca ggaggtatgc gacaacgaac
      421 gctatcaacg aacgctggaa aactttggct atctcatcca gcaggcgacc gtttttggtc
      481 agcatgtcca tgttggctgt gccagtggcg atgacgccat ttatttgctg cacggcttgt
      541 cacggtttgt gccgcacttt attgcccttt ccgccgcgtc gccatatatg cagggaacgg
      601 atacgcgttt tgccccctca cgaccgaata ttttttccgc ctttcctgat aatggcccga
      661 tgccgtgggt cagtaactgg caacaatttg aagccctgtt tcgctgtctg agttacacca
      721 cgatgatcga cagcattaaa gatctgcact gggatattcg ccccagtcct cattttggca
      781 cggtggaggt tcgggtgatg gataccccgt taacccttag ccacgcggta aatatggcgg
      841 gattaattca ggccaccgcc cactggttac tgacggaacg cccgttcaaa catcaggaga
      901 aagattacct gctgtataaa ttcaaccgtt tccaggcctg tcgctatggt ctggaaggcg
      961 tcattaccga tccgcacact ggcgatcgtc gaccgctaac ggaagatacc ttgcgattgc
     1021 tggaaaaaat cgccccttca gcacataaaa ttggcgcatc gagcgcaatt gaagcactgc
     1081 atcgccaggt cgtcagcggt ctgaatgaag cgcagctgat gcgcgatttc gtcgccgatg
     1141 gcggctcgct gattgggctg gtgaaaaagc attgtgagat ctgggccggt gactaagccg
     1201 ggaattgcct tacagaagaa tatccctgac aatggtgttt taacttacat ttaacaatgt
     1261 tatgaaacac cctttagaaa ccttgaccac cgcagcaggc attttgctga tggctttcct
     1321 ctcttgcctg ctgctgcccg cccccgcact ggggctgacg ctggcacaaa aactggtgac
     1381 catgttccat ctgatggatc ttagccagct ttacacttta ttgttttgtc tgtggttttt
     1441 agtgctgggc gctattgagt attttgttct gcgctttatc tggcgacgct ggttctcgct
     1501 ggcggattaa acgtcgatta tcgccgaact tcgcggcata ctttgcttat tctccttcgc
     1561 ctaaaggaac gtttatggat aagcaatcac tgcacgaaac ggcgaaacgc ctagctcttg
     1621 agttaccctt tgtcgagctt tgctggcctt ttggcccgga gttcgatgtt tttaaaattg
     1681 gcggcaagat ttttatgctg tcgtcggagc tacgcggcgt cccctttatc aatctgaagt
     1741 ccgatccaca aaaatccctg ttaaatcagc aaatataccc aagcattaag ccagggtatc
     1801 acatgaataa aaagcactgg atttcggtgt attccggcga ggaaatctcc gaagcgttac
     1861 ttcgcgatct gatcaacgat tcgtggaatc tggtggttga tggtctggct aaacgcgatc
     1921 aaaaaagagt gcgtccaggc taaagcggga atctatagcg catttttctc gcttaccatt
     1981 tctcgttgaa ccttgtaatc tgctggcacg caaaattact tacacatgga gtctttatgg
     2041 atatcatttc tgtcgcctta aagcgtcatt ccactaaggc atttgatgcc agcaaaaaac
     2101 ttaccccgga acaggccgag cagatcaaaa cgctactgca atacagccca tccagcacca
     2161 actcccagcc gtggcatttt attgttgcca gcacggaaga aggtaaagcg cgtgttgcca
     2221 aatccgctgc cggtaattat gtgttcaaca aacgtaaaat acttgatgcc tcgcacgtcg
     2281 tggtgttctg tgcaaaaacc gcgatggacg atgcctggct gaagctggtt gttgaccagg
     2341 aagatgccga tggccgcttt gccacgccgg aagcgaaagc cgcgaacgat aaaggtcgca
     2401 agttcttcgc ctatatgcac cgtaaagatc tgcatgatga tgcagagtgg atggcaaaac
     2461 aggtttatct caacgtcggt aacttcctgc tcggcgtggc ggcgctgggt ctggacgcgg
     2521 tgcccatcga aggttttgac gccgcaatcc tcgatgcaga atttggtctg aaagagaaag
     2581 gctacaccag tctggtggtt gttccggtag gtcatcacag cgttgaagat tttaacgcta
     2641 cgctgccgaa atctcgtctg ccgcaaaaca ccaccttaac cgaagtgtaa ttctctcttg
     2701 ccgggcatct gcccggctat ttcccctcag attctcctga tttgcataac cctgtttcag
     2761 ccgtcatcat aggctgctgt tgtataaagg agacgttatg caggatttaa tatcccaggt
     2821 tgaagattta gcgggtattg agatcgatca caccacctcg atggtgatga ttttcggtat
     2881 tatttttctg accgccgtcg tggtgcatat tattttgcat tgggtggtac tgcggacctt
     2941 cgaaaaacgt gccatcgcca gttcacggct ttggttgcaa atcattaccc agaataaact
     3001 cttccaccgt ttagctttta ccctgcaggg gattatcgtc aatattcagg cggtattctg
     3061 gctgcaaaaa ggcaccgaag cggcagatat tctgactacc tgcgcgcagt tgtggatcat
     3121 gatgtatgcg ctgctttcag tcttctcgtt gctggatgtt attttgaatc tggcgcagaa
     3181 attcccggcg gcatctcagt taccgctgaa agggatattt caggggatta aactgatcgg
     3241 cgcaatcctg gtcggcattt tgatgatctc gctgctgatt ggtcagtcgc cagcgattct
     3301 aatcagcggt cttggtgcaa tggctgccgt gctgatgttg gtatttaaag atccgattct
     3361 tggtctggtg gcaggtattc agctttccgc gaacgatatg ctgaaactgg gcgactggct
     3421 ggaaatgccg aaatacggcg cggatggcgc ggtgatcgat attgggttaa ccaccgtcaa
     3481 agtgcgtaac tgggacaata ccattaccac tattcccacc tggtctctgg tttctgactc
     3541 ctttaaaaac tggagcggga tgtcagcatc tggcgggcga cgtattaagc gcagtatcag
     3601 tattgatgtc accagtattc gttttcttga tgaagacgaa atgcaacgtc tgaataaagc
     3661 gcatttgtta aagccttatt taaccagccg ccatcaggaa attaatgagt ggaatcgcca
     3721 gcaaggttct acggagtcgg tattaaacct gcgccgaatg accaatattg gaacctttcg
     3781 tgcctatctg aacgaatatc tgcgtaacca tccgcggatt cgtaaagata tgaccttaat
     3841 ggtacgccaa ctggctccag gtgataacgg tttaccgctc gagatctacg cgtttactaa
     3901 caccgtggtg tggctggaat atgaaagcat tcaggctgat atattcgatc acatatttgc
     3961 gattgtcgaa gagtttggtc tgcgacttca tcagtcgcca accggcaatg atattcgctc
     4021 tctggcgggt gcatttaagc agtaattaaa aaaaccgctc tcatcgaatg gatgagagcg
     4081 gacatcgttt tgtcggatgt agcgtgaatg ccttatttcc gacgcagcgt tttaaatgcc
     4141 acaaacagga atacaatcca caccggcagc aggatcgctg acaagcgcat atcatccatc
     4201 gtgcacatca gcagcaaaat catgccgagg aaggcgatgc aaagatagtt gccgaacgga
     4261 tagagcagcg ccttaaactg tgtttcacgc ccctgacgtc gcatcgccgc gcgaaaacgc
     4321 agatgcgcca ggcagatcat aatccagttc aacagcaacg ttgctaccac cagcgccatc
     4381 agcagaccaa acgctttttg cggcagcaga tagttgatta acaccaccag cgaagtgatc
     4441 gctccggaaa gcatcagcga gttaatcggc acaccgcgac ggctgacgcg agtcaaaaac
     4501 ttcggcgcat taccctgcac agaaaggcca aacagcatgc ggctgttaga gtaaaccccg
     4561 ctgttataca ccgacagcga tgccactaga atgacgaagt tcagcgcaga agctaccaca
     4621 ttgctgtcga gattatggaa aatcatcaca aacgggctac tgttggattt cacttccacc
     4681 cacggataga gtgccagtaa aaccaccagt gaaccgatat aaaacagcag gatgcgatac
     4741 accacctgat ttaccgcttt tggaatgctt ttttccggat cgcgcgcttc agcggctgta
     4801 atcccaatca gctccagacc gccgaaggag aacataatta ccgccagcga caaaatcagc
     4861 ccattccagc cggtggcgaa gaaaccaccg tagcgccaga ggttgtcgat actggctttc
     4921 tcgccgccgt aaccagaaaa cagcagccac aggccaaagc cgatcatacc gatgatcgcc
     4981 agcactttaa tcagcgcaaa ccagaactcg gtttcgccat ataagcgcac gttcaccaga
     5041 ttaacggcgt tgatgataat aaagaaggcg gcagcccaaa tccacgttgg aacatccggg
     5101 aaccagtact gcatatagat gcccgcagcg gtcagctctg ccattcccac cagcacgaac
     5161 attacccagt agttccagcc agagaggaag cccgcaaacg gtccccagta tttataggca
     5221 aagtgggcaa atgaaccgga taccggctct tcgaccacca tctcgccaag ctggcgcata
     5281 atcaggaaag cgatgatccc ggcgacgccg tagcccagca atacagccgg acccgccatc
     5341 tgaatcgccg ggccaatgcc aagaaacaga ccagtaccaa ttgcgccacc cagcgcaatc
     5401 agttgaatat gacggttatg taatccgcga tgaagcgtcg gctcttgatt cgacgcagta
     5461 tcttccgata cggttgacgc gtttttcacg cctttcccct gtgtgtcttt tttgttgagg
     5521 ggcacctttt aacatttagt gcccatcgtc gcaagacaca atccacacgg ttaaacgagg
     5581 tatcctgctt taattccgta cccgatgtcg gtgcagccac atcagcttat acgccgccgg
     5641 gataataaac agcgacagca aaggtgcggt gatcatgccg ccaatcatcg gtgcggcaat
     5701 ccggctcatc acctctgaac cagcccccgt tccccacaga atcggcagca gaccggcgat
     5761 aatcaccgcc accgtcatcg ctttcgggcg cacgcgcagg accgcgccgt gatacaacgc
     5821 ctcatccagc ttctgctcgc tgaatgtttg tggattattc aacgacggct cggcctctat
     5881 ggcgtgacgt aaatacatca gcatcaccac gccaaattcg gcggcgaccc cggcaagggc
     5941 aataaagccg gtacccgtcg ccacagaaag atgaaagccc atccaccaca gtaaccagat
     6001 gccgcccacc agcgcaaacg gtacgctgct gataatcagc aacgcttcgc ccacccgacg
     6061 gaatgccaga tacaacagca cgaagatgat catcagtgtc atcggcacca tgagtttcag
     6121 cttatggttg gcgcgctcca gtagctcgaa ctgcccggag aatgccacgc tggtgcccgg
     6181 ttttaactgc actttttcag ctatcgcttt ttgcaaatcg tgaaccaccg acaccatgtc
     6241 acgatcgcgg gcatcgatat aaatccagct cgtcgggcgc gcattctcgg ttttcagcat
     6301 cgacggtccg gtagagactt taacgtcggc cacgtctgcc agggtgattt gctgcttcat
     6361 cggcgtcagg atcggcaact ggcgcagtgc ctgcggacta tcgcgccagc tttgcggata
     6421 acgcagatta attggataac gggcaatccc ttccaccgtt tcgccaacca tcgccccgcc
     6481 caccgcagaa gtcacaaaca actgcacatc cgccaccgtc ataccgtaac gtgcggcttt
     6541 ttcacggtta atctcaacgt tgatatagcg cccaccttcc agccgctcag caagcgcaga
     6601 agctacgcct ggcaccgttc gcgccacttc ttcaatttgc tcagccatcg cgtcgatatc
     6661 cgccagcaca gtgccggaaa ctttaatgcc gatggggctt ttaatgccgg ttgagagcat
     6721 atcgatacgg ttacgaattg gcggcaccca cagattcgcc agccccggca gacgcacggt
     6781 gttatccagt tcctcaatga ttttgtccat tgtcatgcct ggccgccact gatcctgtgg
     6841 cttaagctgg atggtcgttt ctaccatctc cagcggagca gaatcggtgg cggtttccgc
     6901 tttcccggtt ttgccaaata cccgcgccac ttcaggtacg ctcataatta gcttgtcggt
     6961 tttttgcagc atactcgccg cctctgctgc ggaaatcccc ggcagcgtcg atggcatata
     7021 caacaagtcg ccttcattga tctgcggtaa aaattccccg ccaactttat tgagcggcca
     7081 gagaaccgtc agcaccgaaa gcgccgccac cagcagcgtg gttttcggcc agtgcagtac
     7141 tttcagcaac agcggatgat aaacacgaat caaaaagcga ttgagcggat tactgctttc
     7201 cggcggaatt ttgccacgga tccagtaacc catcaggata gggatcacta cgatcgccag
     7261 cagcgccgca cccgccatcg catacgtttt ggtgaacgcc aacgggccaa acagacgccc
     7321 ttcctgccct tccagggtga agatcgggat aaacgacaac gtgataatca gcagactgat
     7381 aaacagcgcc ggccccactt caacagacgc atcggtgatc acctgccagc gcgttttatt
     7441 atccagcgtg gcgtcaggat gctggtgctg ccactcttcc agctgtttat gcgcattctc
     7501 gatcatgacg atagcggcat cgaccatcgc cccgacggca atcgcaatgc cacccagcga
     7561 cataatattg gcattcagcc cctggaaggg catgacaata aaagcaatac acaaccccag
     7621 cggcaacgaa ataatcgcca ccagcgccga gcgcacatgc cagagaaacg gcgcgcagac
     7681 taccgccacc acaataaact cttccagcaa cttgccgctg aggttgtcga tggcccggtc
     7741 aatgagctgg ctgcggtcgt atgttgtaac tatctccacg ccttccggca gactactttt
     7801 cagcgtttcc agtttgtcct tcacggcggc gatcacttct cgggcgtttt tgccggatcg
     7861 caggatcacc accccgcccg ccacttcgcc ttcgccgttt agttcggcaa tgccccggcg
     7921 catcttcggg ccaatctgga ccttcgcaac atcgcgcaga taaacgggca cgccattttc
     7981 actggctttt aaaacgatgt gattaaagtc gtcgagcgtt tgtagatagc cgctggcgcg
     8041 caccatatat tctgcttccg ccagttcgat tgacgaaccg cccgcttcct ggtttgaagc
     8101 atccagcgcg ctttttactt cggcgagact gatgccatac tgcgccaggc gctgaggatc
     8161 gataaccacc tgatactctt tcaccacacc gcctaccgac gccacttccg caacgtcagg
     8221 aatggttttc agctcatatt tgagaaacca gtcctgcaat gagcgtaaat cggcaagatc
     8281 gtgcttaccg ctgcgatcca ccagtgcata ttcatagatc cagccaacac ccgtggcatc
     8341 tggtcccagc tcggcgctga ctcccgcagg cagcttaccc tgtacctggt tgaggtactc
     8401 cagcacccgc gaacgcgccc agtacggatc ggtgccatct tcgaaaatga catacacata
     8461 agagtcgcca aactgcgaga aaccacgcac agtcttcgcg ccaggcaccg acaacatggt
     8521 ggtggttagc ggataagtca cctgattttc aacgatttgc ggtgcctggc cgggatagct
     8581 ggttttaata atcacctgca catcggagag atccggcaac gcatccactg gcgtattaat
     8641 gatggtccag gtgccccaga tgctcagaaa caacgcgccc atcagcacca aaaaacggtt
     8701 cgccaccgag cgacgaataa tccattcaat cattggttat tccctcaatg cgcatgggta
     8761 gcactttcag agcgcatccg ctccagtgcg ccagaaatat tggcttcgga atcaatcagg
     8821 aacaggccgc cggaaaccac cttttcacct tccgccagac cagagcgtaa tgcggtgacg
     8881 ccttgcgatg cctggaaaac agcaacgcgt ttcggtacaa agcgcccgtc ggcatcaacg
     8941 gtaatcaccc gctgttcgtt gccggtatca atcagcgcct gtgacggaat gagcagcatc
     9001 ggttcgctgg cggtgttgag ttgcaaccag gcgttcatgc ccggttttag cgcctcgtcg
     9061 gcgttgtcga cttccagacg cagctgcagc gtgcgggtcg cggcatccac gccaggtagc
     9121 agcgtccatt tgcggatggt gagtgtttta tccggtcgcg ccggaacggt gagggtaaac
     9181 tgcgaggcat ctttcaccag ccaggcgatg gactccggga tcgcagcagt gacccacacc
     9241 gggtccatac cctgaattcg cgattacgcg caatccccct gagaaccgcc atgaacaagg
     9301 gtttcatgat ttgtgatttt agtttggtac gcaatttggt acacaacact attttcactt
     9361 cagcgggtag tcatcaaact caccataacg agca
//
LOCUS       DAJTRC010000131         9340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.131, whole genome shotgun sequence.
ACCESSION   DAJTRC010000131 DAJTRC010000000
VERSION     DAJTRC010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.131"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            392..2023
                     /gene="oppA"
                     /locus_tag="OO357_003541"
     CDS             392..2023
                     /gene="oppA"
                     /locus_tag="OO357_003541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein OppA"
                     /protein_id="HCR5888359.1"
                     /translation="MTNITKRSLVAAGVLAALMAGNVALAADVPAGVTLAEKQTLVRN
                     NGSEVQSLDPHKIEGVPESNISRDLFEGLLVSDLDGHPAPGVAESWDNKDAKVWTFHL
                     RKDAKWSDGTPVTAQDFVYSWQRSVDPNTASPYASYLQYGHIAGIDEILEGKKPITDL
                     GVKAIDDHTLEVTLSEPVPYFYKLLVHPSTSPVPKAAIEKFGEKWTQPGNIVTNGAYT
                     LKDWVVNERIVLERSPTYWNNAKTVINQVTYLPIASEVTDVNRYRSGEIDMTYNNMPI
                     ELFQKLKKEIPDEVHVDPYLCTYYYEINNQKPPFNDVRVRTALKLGMDRDIIVNKVKA
                     QGDMPAYGYTPPYTDGAKLTQPEWFGWSQEKRNEEAKKLLAEAGYTADKPLTINLRYN
                     TSDLHKKLAIAASSLWKKNIGVNVKLVNQEWKTFLDTRHQGTFDVARAGWCADYNEPT
                     SFLNTMLSNSSMNTAHYKSPAFDSIMAETLKVTDEAQRTALYTKAEQQLDKDSAIVPV
                     YYYVNARLVKPWVGGYTGKDPLDNTYTRNMYIVKH"
     gene            2109..3029
                     /gene="oppB"
                     /locus_tag="OO357_003542"
     CDS             2109..3029
                     /gene="oppB"
                     /locus_tag="OO357_003542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppB"
                     /protein_id="HCR5888360.1"
                     /translation="MLKFILRRCLEAIPTLFILITISFFMMRLAPGSPFTGERTLPPE
                     VMANIEAKYHLNDPIMTQYFSYLKQLAHGDFGPSFKYKDYSVNDLVASSFPVSAKLGA
                     AAFFLAVILGVSAGVIAALKQNTKWDYTVMGLAMTGVVIPSFVVAPLLVMIFAIILHW
                     LPGGGWNGGALKFMILPMVALSLAYIASIARITRGSMIEVLHSNFIRTARAKGLPMRR
                     IILRHALKPALLPVLSYMGPAFVGIITGSMVIETIYGLPGIGQLFVNGALNRDYSLVL
                     SLTILVGALTILFNAIVDVLYAVIDPKIRY"
     gene            3044..3952
                     /gene="oppC"
                     /locus_tag="OO357_003543"
     CDS             3044..3952
                     /gene="oppC"
                     /locus_tag="OO357_003543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000979653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter permease OppC"
                     /protein_id="HCR5888361.1"
                     /translation="MMLSKKNSETLENFSEKLEVEGRSLWQDARRRFMHNRAAVASLI
                     VLVLIALFVILAPMLSQFAYDDTDWAMMSSAPDMESGHYFGTDSSGRDLLVRVAIGGR
                     ISLMVGVAAALVAVVVGTLYGSLSGYLGGKVDSVMMRLLEILNSFPFMFFVILLVTFF
                     GQNILLIFVAIGMVSWLDMARIVRGQTLSLKRKEFIEAAQVGGVSTPGIVIRHIVPNV
                     LGVVVVYASLLVPSMILFESFLSFLGLGTQEPLSSWGALLSDGANSMEVSPWLLLFPA
                     GFLVVTLFCFNFIGDGLRDALDPKDR"
     gene            3964..4977
                     /gene="oppD"
                     /locus_tag="OO357_003544"
     CDS             3964..4977
                     /gene="oppD"
                     /locus_tag="OO357_003544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppD"
                     /protein_id="HCR5888362.1"
                     /translation="MSVIETATVPLAQQQADALLNVKDLRVTFSTPDGDVTAVNDLNF
                     SLRAGETLGIVGESGSGKSQTAFALMGLLAANGRIGGSATFNGREILNLPERELNKLR
                     AEQISMIFQDPMTSLNPYMRVGEQLMEVLMLHKNMSKAEAFEESVRMLDAVKMPEARK
                     RMKMYPHEFSGGMRQRVMIAMALLCRPKLLIADEPTTALDVTVQAQIMTLLNELKREF
                     NTAIIMITHDLGVVAGICDKVLVMYAGRTMEYGNARDVFYQPVHPYSIGLLNAVPRLD
                     AEGETMLTIPGNPPNLLRLPKGCPFQPRCPHAMEICSSAPPLEEFTPGRLRACFKPVE
                     ELL"
     gene            4974..5978
                     /gene="oppF"
                     /locus_tag="OO357_003545"
     CDS             4974..5978
                     /gene="oppF"
                     /locus_tag="OO357_003545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide/oligopeptide ABC transporter
                     ATP binding protein OppF"
                     /protein_id="HCR5888363.1"
                     /translation="MNAVTEGRKVLLEIADLKVHFEIKDGKQWFWQPPKTLKAVDGVT
                     LRLYEGETLGVVGESGCGKSTFARAIIGLVKATDGHVAWLGKELLGMKPDEWRAVRSD
                     IQMIFQDPLASLNPRMTIGEIIAEPLRTYHPKMSRQEVRERVKAMMLKVGLLPNLINR
                     YPHEFSGGQCQRIGIARALILEPKLIICDEPVSALDVSIQAQVVNLLQQLQREMGLSL
                     IFIAHDLAVVKHISDRVLVMYLGHAVELGTYDEVYHNPLHPYTRALMSAVPIPDPDLE
                     KNKTIQLLEGELPSPINPPSGCVFRTRCPIAGPECAKTRPVLEGSFRHAVSCLKVDPL
                     "
     gene            complement(6031..6360)
                     /locus_tag="OO357_003546"
     CDS             complement(6031..6360)
                     /locus_tag="OO357_003546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309775.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciU family protein"
                     /protein_id="HCR5888364.1"
                     /translation="MDMDLNNRLTEDETLEQAYDIFLELAADNLDPADVLLFNLQFEE
                     RGGAELFDPAEDWQEHVDFDLNPDFFAEVVIGLADSEDGEISDVFARILLCREKDHKL
                     CHIIWRE"
     gene            complement(6395..7855)
                     /gene="cls"
                     /locus_tag="OO357_003547"
     CDS             complement(6395..7855)
                     /gene="cls"
                     /locus_tag="OO357_003547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005126413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HCR5888365.1"
                     /translation="MTTVYTLVSWLAILGYWLLIAGVTLRILMKRRAVPSAMAWLLII
                     YILPLVGIIAYLAVGELHLGKRRAERARAMWPSTAKWLNDLKACKHIFAEENSSVAAP
                     LFKLCERRQGIAGVKGNQLQLMTESDDVMQALIRDIQLARHNIEMVFYIWQPGGMADQ
                     VAESLMAAARRGIHCRLMLDSAGSVAFFRSPWPELMRNAGIEVVEALKVNLMRVFLRR
                     MDLRQHRKMIMIDNYIAYTGSMNMVDPRYFKQDAGVGQWIDLMARMEGPIATAMGIIY
                     SCDWEIETGKRILPPPPDVNIMPFEQASGHTIHTIASGPGFPEDLIHQALLTAAYSAH
                     EYLIMTTPYFVPSDDLLHAICTAAQRGVDVSIILPRKNDSMLVGWASRAFFTELLAAG
                     VKIYQFEGGLLHTKSVLVDGELSLVGTVNLDMRSLWLNFEITLAIDDKGFGADLAAVQ
                     DDYISRSRLLDARLWLKRPLWQRVAERLFYFFSPLL"
     gene            7998..8171
                     /locus_tag="OO357_003548"
     CDS             7998..8171
                     /locus_tag="OO357_003548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciY family protein"
                     /protein_id="HCR5888366.1"
                     /translation="MKRSRTEVGRWRMQRQASRRKSRWLEGQSRRNMRIHSIRKCILN
                     KQRNSLLFAIYNI"
     gene            complement(8226..>9340)
                     /gene="kch"
                     /locus_tag="OO357_003549"
     CDS             complement(8226..>9340)
                     /gene="kch"
                     /locus_tag="OO357_003549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="voltage-gated potassium channel protein"
                     /protein_id="HCR5888367.1"
                     /translation="IFHIKAFSELDLSLLANAPLFMLGVFLVLNSIGLLFRAKLAWAI
                     SIILLLIALIYTLHFYPWLKFSIGFCIFTLVFLLILRKDFSHSSAAAGTIFAFISFTT
                     LLFYSTYGALYLSEGFNPRIESLMTAFYFSIETMSTVGYGDIVPVSESARLFTISVII
                     SGITVFATSMTSIFGPLIRGGFNKLLKGNNHTMHRKDHFIVCGHSILAINTILQLNQR
                     GQNVTVISNLPEDDIKQLEQRLGDNADVIPGDSNDSSVLKKAGIDRCRAILALSDNDA
                     DNAFVVLSAKDMSSDVKTVLAVSDSKNLNKIKMVHPDIILSPQLFGSEILARVLNGEE
                     INNDMLVSMLLNSGHGIFSDNDEQETKADSKESAQK"
BASE COUNT         2424 a         2202 c         2345 g         2369 t
ORIGIN      
        1 tgaaagatgt ttgttaaggc atggatgcaa gctatagatt ctgatacggt caataaaaga
       61 gaatcgctta acaattttgc aaaatgtatt ggcgagtaag aaccgcattt ggtactttcc
      121 gggcaaccgc cagacgattc tttattggta atgagaataa ttaacaatta aagagcgtcg
      181 cgaaagaata atgtgtctcg acaggggaga cacagtacga atcgacataa ggtgatcgtc
      241 tgaatcacca gaataaataa agtcggtgat agtaatacgt aacgataaag taacctgaca
      301 gcagaaagtc tccgagcttg tgcagggtcc caatccggga ttacacatgc tggttaatac
      361 cagtaattat aatgagggag tccaaaaaac aatgaccaac atcaccaaga gaagtttagt
      421 agcagctggc gttctggctg cgctaatggc agggaatgtc gcgctggcag ctgatgtacc
      481 cgcaggcgtc acactggcgg aaaaacaaac actggtacgt aacaatggtt cagaagttca
      541 gtcattagat ccgcacaaaa ttgaaggtgt tccggagtct aatatcagcc gagacctgtt
      601 tgaaggctta ctggtcagcg atcttgacgg tcatccagca cctggcgtcg ctgaatcctg
      661 ggataataaa gacgcgaaag tctggacctt ccatttgcgt aaagatgcga aatggtctga
      721 tggcacgcca gtcacagcac aagactttgt gtatagctgg caacgttctg ttgatccgaa
      781 cactgcttct ccgtatgcca gttatctgca atatgggcat atcgccggta ttgatgaaat
      841 tcttgaaggg aaaaaaccga ttaccgatct cggcgtgaaa gctattgatg atcacacatt
      901 agaagtcacc ttaagtgaac ccgttccgta cttctataaa ttacttgttc acccatcaac
      961 ttcaccggtg ccaaaagccg ctatcgagaa attcggcgaa aaatggaccc agcctggtaa
     1021 tatcgtcacc aacggtgcct ataccttaaa agattgggtc gtaaacgaac gaatcgttct
     1081 tgaacgcagc ccgacctact ggaacaacgc gaaaaccgtt attaaccagg taacctattt
     1141 gcctattgct tctgaagtta ccgatgtcaa ccgctaccgt agtggtgaaa tcgacatgac
     1201 gtataacaac atgccgatcg aattgttcca gaagctgaaa aaagagatcc cggacgaagt
     1261 tcacgttgat ccatacctgt gcacttacta ttacgaaatt aacaaccaga aaccgccatt
     1321 caacgatgtg cgtgtgcgta ccgcactgaa actaggtatg gaccgcgata tcattgttaa
     1381 taaagtgaaa gcgcagggcg acatgcccgc ctatggttat actccaccgt atactgatgg
     1441 cgcaaaattg actcagccag aatggtttgg ctggagccag gaaaaacgta acgaagaagc
     1501 gaaaaaactg ctggctgaag cgggttatac cgcagacaaa ccgttgacca tcaacctgcg
     1561 gtataacacc tccgatctgc ataaaaagct ggcgattgct gcctcttcat tgtggaagaa
     1621 aaacattggt gtaaacgtca aactggttaa ccaggagtgg aaaacgttcc tcgacacccg
     1681 tcaccagggc acttttgatg tggcccgtgc aggctggtgt gcagactaca acgaaccaac
     1741 ttccttcctg aacaccatgc tgtcgaacag ctcgatgaat accgcgcatt ataagagccc
     1801 ggcctttgac agcattatgg cggaaacgct gaaagtgact gacgaggcgc agcgcacagc
     1861 tctgtacact aaagcagaac aacagctgga taaggattcg gccattgttc ctgtttatta
     1921 ctacgtgaat gcgcgtctgg tgaaaccgtg ggttggtggc tataccggca aagatccgct
     1981 ggataatacc tatacccgga atatgtacat tgtgaagcac taatggcaat acgtggggca
     2041 ggagtgtcct gctccacggt gtctgatttt tatcgcatta cagaaggcac aggccagaag
     2101 gtagggcaat gttaaaattt attctacgtc gttgtctgga agcgattccg acgctattta
     2161 ttcttattac tatttcgttc tttatgatgc gcctcgcacc gggaagccct tttaccggcg
     2221 aacgtacttt accgccagaa gtgatggcca atatcgaagc gaaatatcat cttaatgatc
     2281 cgatcatgac acagtatttc agctacctga aacaactggc gcatggcgat ttcggtccat
     2341 cgtttaaata taaagattat tcggtcaatg acctggtggc atccagtttt cccgtttctg
     2401 ccaaactggg agccgccgca tttttccttg cggtaatact gggtgttagt gctggcgtta
     2461 ttgccgcatt aaaacaaaac accaaatggg actataccgt gatggggctg gcaatgaccg
     2521 gggttgttat ccccagtttt gtggttgcgc cattattagt catgatattt gcgatcattt
     2581 tgcattggct gccgggcggt ggctggaatg gtggggcgct taaattcatg atattgccga
     2641 tggtggcgtt gtcactcgcc tatatcgcca gtattgcgcg tattacccgt ggctctatga
     2701 ttgaagtatt acactccaac tttattcgta ctgcccgggc gaaagggtta cctatgcggc
     2761 ggatcatttt acgccatgca ttaaaacctg ctctgttacc cgtgctctcc tatatgggcc
     2821 ccgcatttgt cggcattatt accggttcta tggtcatcga aaccatttat ggtttgccgg
     2881 ggattgggca actgttcgtt aatggcgcat tgaaccgtga ctattcctta gtgttaagcc
     2941 tgaccatcct ggttggtgcg ttaaccattt tgtttaatgc cattgtcgat gtgctatatg
     3001 cggttatcga cccgaaaatc cgttactgat actggagctc gcgatgatgt taagtaagaa
     3061 aaacagcgag acgctggaaa atttcagtga aaagctggag gtcgaagggc gcagcttgtg
     3121 gcaggacgca cgtcgacgtt ttatgcataa ccgtgcggcg gttgccagtc tgatagtgct
     3181 ggtgctgatc gcgttatttg taatcctggc accgatgctt tcccagtttg cctatgacga
     3241 tactgactgg gcgatgatgt ccagcgcccc ggatatggag tccggtcact actttggtac
     3301 tgactcatcc ggtcgcgacc tgcttgtgcg cgttgcgatt ggcgggcgta tctcactcat
     3361 ggtcggtgtt gctgcggcac tggtggcagt ggtcgtgggg acactttatg gttcgctttc
     3421 cggttatctg ggcggtaaag tggattcggt aatgatgcgt ctgctggaaa tcctcaactc
     3481 cttcccattc atgttcttcg tcattttgct ggtgaccttt ttcggccaaa acatcctgct
     3541 gattttcgtg gcgattggca tggtttcctg gctggatatg gctcgtattg tgcgtgggca
     3601 aaccctgagt ctgaagcgca aagagtttat tgaggcggca caagttggcg gtgtatcgac
     3661 gccgggcatt gttattcgcc acattgtgcc gaacgtactc ggtgtggtgg tggtctacgc
     3721 atcgctactg gtgcccagca tgatcctctt tgaatctttc cttagcttcc tggggttggg
     3781 tacgcaagag ccgttaagca gctggggggc attgctgagt gatggcgcga actcgatgga
     3841 agtctctcca tggttactgt tgttcccagc gggattcctt gtggtgacac tgttttgttt
     3901 caactttatc ggcgatggct tgcgtgatgc cctcgacccg aaagatcgtt aaggagtgca
     3961 gccatgagcg taattgaaac tgcaactgtg ccgctcgcac aacaacaggc tgacgcactg
     4021 ctgaacgtga aagatttgcg tgtcaccttt agtaccccgg acggcgacgt cacggcggtc
     4081 aatgatttaa atttttccct acgtgccgga gaaacgctgg gcattgtagg tgagtctggt
     4141 tcgggtaaat cgcaaactgc atttgcgttg atgggcctgt tggctgccaa cggacgcatt
     4201 ggcggatcgg caaccttcaa tgggcgtgaa atcctcaatt tgccagagcg tgaactcaat
     4261 aaactgcgcg ctgaacaaat ctcaatgatt tttcaggacc caatgacttc gttgaatccc
     4321 tatatgcgcg tcggtgagca gttgatggaa gtgctgatgc tgcataagaa catgagcaaa
     4381 gctgaggcgt ttgaagagtc ggtgcggatg ctcgatgcgg taaaaatgcc ggaagcacgt
     4441 aaacgcatga aaatgtaccc gcacgaattt tctggcggca tgcgtcagcg agtcatgatt
     4501 gcgatggcat tgctatgtcg acctaagttg ctgattgcgg atgaacccac tacagcgctg
     4561 gacgtcaccg tacaggcgca gatcatgacg ctattgaatg aactgaagcg ggaatttaat
     4621 actgccatca ttatgatcac ccacgatctt ggcgtggtgg cggggatctg tgacaaagtg
     4681 ctggtaatgt acgccgggcg cacgatggaa tatggcaatg cgcgcgatgt cttttatcaa
     4741 cccgttcatc cttattctat cggtttgctc aacgcggtgc cgcgtcttga tgcggaaggt
     4801 gaaacaatgt tgaccatccc gggtaatccg ccaaacttgc tgcgattacc gaaaggttgc
     4861 ccgttccagc cacgttgtcc acatgcgatg gaaatttgta gtagcgcacc gccgctggaa
     4921 gagtttacgc ctggccgtct gcgtgcttgc tttaaaccgg tggaggaact gttatgaatg
     4981 ctgtaactga aggaagaaaa gtcctccttg aaatcgccga tcttaaagtg cactttgaaa
     5041 tcaaagatgg caaacagtgg ttctggcaac cgccgaaaac gctcaaagcc gtcgatggtg
     5101 tcactcttcg cctgtatgaa ggggaaacat taggtgtggt aggggaatcg ggatgcggta
     5161 agtccacctt tgctcgcgcc atcatcggtt tggtcaaggc gaccgacggt catgttgcct
     5221 ggttaggtaa agagttgctg ggcatgaagc ccgatgaatg gcgtgccgtt cgcagtgata
     5281 ttcagatgat tttccaggat ccgttggcat cgctaaaccc gcgtatgacc atcggcgaga
     5341 tcatcgctga accactgcgt acttatcatc cgaaaatgtc acgccaggaa gttcgcgagc
     5401 gcgtgaaggc gatgatgctg aaagtcgggt tattgcctaa cctgattaac cgctatccgc
     5461 atgagttctc cggtgggcag tgccagcgta tcgggattgc tcgtgctctt attcttgaac
     5521 cgaagctgat tatctgcgat gagccggttt cggcgctgga cgtgtcaatt caggcgcagg
     5581 tggttaacct gctccagcag ctgcaacgtg agatgggatt gtcattaatt tttattgctc
     5641 atgacctggc cgtggtaaaa cacatttccg atcgtgtgtt ggtgatgtat ctcggccatg
     5701 cagtagaact ggggacctat gatgaggtct accacaatcc actacatcct tacaccaggg
     5761 cattgatgtc ggcagtcccc atacctgatc cggatctgga gaagaacaaa accatccagt
     5821 tactggaagg ggaattaccg tcgccgatca acccgccttc cggttgtgtt ttccgtaccc
     5881 gttgcccgat tgccggtccg gagtgcgcca aaacacgtcc tgttctggag gggagtttca
     5941 gacacgccgt ttcttgcctg aaagtcgatc cgctttaaaa acaataaggg ctgacagttg
     6001 tcagcccttt ttcacgctaa aagcgattat ttattcccgc cagatgatat ggcaaagttt
     6061 gtgatctttt tcgcggcata acagaatgcg ggcgaaaaca tcgctgattt caccatcttc
     6121 actgtccgcc agaccaatca ccacttcggc aaaaaagtcc gggttcaaat cgaaatcaac
     6181 atgttcctgc cagtcttccg ccggatcgaa taactccgcg ccgccgcgct cttcaaactg
     6241 aagattgaac agcagaacat cagctggatc gagattgtcg gcagccagtt cgagaaaaat
     6301 atcgtaggcc tgctcaagcg tttcatcttc agtcaggcga ttgtttagat ccatatccat
     6361 agtcactacc tgtttaacct ctgttggcga cgttttacag caacgggctg aagaagtaaa
     6421 acagtcgctc ggcgacacgt tgccataatg gacgttttag ccataaacgg gcatcgagca
     6481 gacgtgaacg cgaaatataa tcgtcctgaa cggcggcaag gtctgcacca aaacctttat
     6541 cgtcgattgc caaggtaatc tcgaaattca gccacagact acgcatatca aggttaacgg
     6601 tgccaaccag acttagttcg ccatcgacca gcacgctctt ggtatgcagt aacccgcctt
     6661 caaactgata aattttaacc ccagcagcaa gcagttccgt aaagaatgcg cgactggccc
     6721 agccgaccag catcgagtca ttttttcgcg gaaggataat actgacatcc accccgcgct
     6781 gcgccgccgt gcaaatcgca tgaagtaaat catcgcttgg cacaaagtag ggcgtggtca
     6841 tgatcaaata ttcatgcgcc gaataagccg cagtcaataa tgcctggtga atgagatctt
     6901 ccggaaagcc ggggccagaa gcaattgtgt gaatggtgtg accgctggcc tgttcaaacg
     6961 gcataatatt gacatctggt ggtggcggca gaatacgttt tccggtttca atctcccagt
     7021 cgcaggaata aataatcccc atcgcggtgg cgatggggcc ttccatacgc gccatcagat
     7081 caatccattg ccctacgccc gcatcttgtt tgaagtagcg aggatcgacc atattcatgc
     7141 tgccggtgta cgcgatgtaa ttatcgatca tgatcatctt gcgatgttgg cgcaggtcca
     7201 tacggcgtaa aaacacacgc atcagattga cctttaaggc ttcgaccact tcaataccgg
     7261 cattacgcat tagctcgggc cacgggctgc ggaaaaaagc cacactcccg gcggagtcga
     7321 gcatcaatcg gcaatgaata ccacgccgtg cagccgccat taatgattca gccacctggt
     7381 ccgccatgcc gccgggctgc cagatataaa acaccatctc aatattatgg cgcgcgagct
     7441 ggatgtcgcg gattaacgcc tgcatcacat catctgactc ggtcatcagc tgtagctgat
     7501 tccctttgac cccagcgatc ccctgacgac gctcgcaaag cttgaataat ggcgcagcga
     7561 cactgctatt ttcttcggcg aagatatgct tacaggcttt aaggtcgtta agccattttg
     7621 cggtggaagg ccacatcgct ctggcgcgct cagcgcggcg tttgcctaaa tggagctcgc
     7681 caacggcaag ataggcaata attccgacta acggcagaat gtaaataatc aacagccagg
     7741 ccatcgcgga gggaactgcg cgtcgtttca ttagaatgcg taaagttacg cctgcaatga
     7801 gcaaccagta tcccagaatg gccaaccaac tcaccaacgt ataaacggtt gtcatagatt
     7861 agaaatcctt ttgaaagcgc attgttatga gtttacgcat atggattcat ctggcaaata
     7921 aaaacgcggg aaaagcactg gtcagcgtcg ggggcggggg tataatgacc attctgttat
     7981 tgcatagagt agttaacatg aagcggagta gaacggaagt ggggcgctgg cgcatgcagc
     8041 gtcaggctag ccgacgtaaa tcgcgttggc ttgaggggca atcgcgccga aatatgcgta
     8101 tccacagcat caggaagtgc attctaaaca aacagcgtaa ctcgttattg tttgcgatct
     8161 acaatatcta aatgtactgg gcaccattgc ggtgcccaat aagtttactc ttccgtcttt
     8221 aaactctatt tttgcgccga ttctttactg tcagctttcg tttcttgttc atcgttatcg
     8281 ctgaaaatac catgacctga gttcaacaac attgaaacga gcatatcatt attaatctct
     8341 tcaccattta aaactcgcgc cagaatttcg ctgccaaaca gttgcggtga gagaatgata
     8401 tccggatgta ccatcttaat cttattcagg tttttgctat cactgacggc gagaactgtt
     8461 ttgacatcac tgctcatatc ttttgccgag agtacaacaa acgcgttatc tgcatcgtta
     8521 tcactcagcg ccagaatggc ccggcaacga tcgattcccg ctttctttaa tactgaactg
     8581 tcattactgt caccggggat aacatcagcg ttatcgccta agcgttgctc aagttgcttg
     8641 atatcatctt caggcaagtt gctgataacc gttacgtttt gtccgcgttg attcagttgc
     8701 aggatcgtat tgatggcgag aatcgaatgt ccgcaaacaa taaaatgatc tttacgatgc
     8761 attgtatgat tgtttccttt taaaagtttg ttgaatcccc cgcggataag cggaccaaaa
     8821 attgaggtca tagatgtggc aaaaacggta atgccggaaa taatgaccga aatagtgaac
     8881 aatcgtgctg attcagaaac agggacaata tctccatagc cgacggttga catggtttct
     8941 atcgaaaaat agaacgcggt catcaaactt tctattcgcg gattaaaacc ttcgcttaaa
     9001 taaagcgcac cgtaggttga gtaaaacagt aatgtcgtga aactaataaa tgcaaaaatt
     9061 gtcccggctg cggcgctact gtgggagaag tctttgcgca gtatcagcaa aaacaccagc
     9121 gtaaaaatgc aaaatccaat actaaatttc agccagggat aaaaatgcag ggtgtaaatt
     9181 agcgctatca acaacaaaat gatactgatt gcccatgcga gctttgcccg gaacagtaag
     9241 ccaatggagt tcaggacaag aaagacgccg agcataaata gtggggcgtt tgccagcaag
     9301 gagagatcca actctgaaaa tgctttgata tggaaaatat
//
LOCUS       DAJTRC010000132         9332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.132, whole genome shotgun sequence.
ACCESSION   DAJTRC010000132 DAJTRC010000000
VERSION     DAJTRC010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.132"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(42..521)
                     /gene="xdhC"
                     /locus_tag="OO357_003550"
     CDS             complement(42..521)
                     /gene="xdhC"
                     /locus_tag="OO357_003550"
                     /EC_number="1.17.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase iron sulfur-binding
                     subunit XdhC"
                     /protein_id="HCR5888368.1"
                     /translation="MNHSETITTECTINGMPFQLHAAPGTPLSELLREQGLLSVKQGC
                     CVGECGACTVLVDGTAIDSCLYLAAWAEGKEIRTLEGEAKGGKLSHVQQAYAKSGTVQ
                     CGFCTPGLIMATTAMLAKPREKPLTITEIRRGLAGNLCRCTGYQMIVNTVLDCEKTK"
     gene            complement(518..1396)
                     /gene="xdhB"
                     /locus_tag="OO357_003551"
     CDS             complement(518..1396)
                     /gene="xdhB"
                     /locus_tag="OO357_003551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase FAD-binding subunit XdhB"
                     /protein_id="HCR5888369.1"
                     /translation="MFDFASYHRAATLADAINLLADNPQAKLLAGGTDVLIQLHHHND
                     RYRHIVDIHNLAELRGITLAEDGSLRIGSATTFTQLIEDPITQRHLPALCAAATFIAG
                     PQIRNVATYGGNICNGATSADSATPTLIYDAKLEIHSPRGVRFVPINGFHTGPGKVSL
                     EHDEILVAFHFPPQPKEHAGSAHFKYAMRDAMDISTIGCAAHCRLDNGNFSELRLAFG
                     VAAPTPIRCQHAEQTAQNAPLNLQTLEAISESVLQDVAPRSSWRASKEFRLHLIQTMT
                     KKVISEAVAAAGGKLQ"
     gene            complement(1407..3704)
                     /gene="xdhA"
                     /locus_tag="OO357_003552"
                     /pseudo
     CDS             complement(1407..3704)
                     /gene="xdhA"
                     /locus_tag="OO357_003552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708647.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase molybdenum-binding
                     subunit XdhA"
     gene            4119..4874
                     /locus_tag="OO357_003553"
     CDS             4119..4874
                     /locus_tag="OO357_003553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311765.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HCR5888370.1"
                     /translation="MSAGRLNKKSLGIVMFLSVGLLLAGCSGSKSSDTGTYSGSVYTV
                     KRGDTLYRISRTTGTSVKELARLNGISPPYTIEVGQKLKLGGAKSSSSTRKSTAKSTT
                     KTASVTPSSAVPKSSWPPVGQRCWLWPTTGKVIMPYSTADGGNKGIDISAPRGTPIYA
                     AGAGKVVYVGNQLRGYGNLIMIKHSEDYITAYAHNDTMLVNNGQSVKAGQKIATMGST
                     DAASVRLHFQIRYRATAIDPLRYLPPQGSKPKC"
     gene            4953..5026
                     /locus_tag="OO357_003554"
     tRNA            4953..5026
                     /locus_tag="OO357_003554"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4985..4987,aa:Gly,seq:ccc)
     gene            5161..6771
                     /locus_tag="OO357_003555"
     CDS             5161..6771
                     /locus_tag="OO357_003555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5888371.1"
                     /translation="MLKSRTYLYQRNGVFYIRLRMKTTSRLTASLPSHNRYKLASVSL
                     RTKDRRTAMAHSRHIKSALKAIHADNPNASYEELREHLKTIVEWELSVSRDDLNDPES
                     YQLYVDQYDDIKSNLREAVATERLTVDQHRYINDVIGVLKACQDRLNGDSSGLLSYLE
                     PETGSLRPSVSLSVLAEPEVPEPKALTLASLIEQYEQENAQNWKPATLSENRASHSTL
                     IEIFDYLDIQDVGKATRADMLRVREVLQQLPKNRKQRFKSMPLSDLLNRESKTDCLDV
                     VTINNKYLIKMAAVFKWAVRNDLIAKNLTEGLELKVPQRKASDARDAFSPEQVGQLLV
                     AAKAYSQKTSGKPYHYYVTALAAITGARLNEVAQLQVKDVRTTEAGTVFIHINEDDSS
                     LPGKSIKNAHSDRCVPLVDGAYGFVLADFMSLVEDRRKTEGDNAMVFNGLKLMKNGYG
                     EQVSKWFNRTLLPKVLADRSGLAFHSFRHTVATQLKQHGVELAYAQAIMGHSSGSITY
                     DRYAKEVEVETLKEKLAESLSVKKIDGK"
     gene            6803..7410
                     /locus_tag="OO357_003556"
                     /pseudo
     CDS             6803..7410
                     /locus_tag="OO357_003556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000237836.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            8341..9087
                     /locus_tag="OO357_003557"
     CDS             8341..9087
                     /locus_tag="OO357_003557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HCR5888372.1"
                     /translation="MSQQEISIINLDQLVSMTSVEIAELTGKEHKHVLRDIRNMVEEL
                     NGAKTEHCSTLSSELNGSKFGLVGEEVYKDAKGESRTMYRLDRKHTFILVAGYSVHLR
                     AKCYDHIQTLERRVLQLEDQKKRAAIQSANRRGVTWGDYCKTYGLPAQKLMTALLQHR
                     GLFRKNPISNEWSVNPKYSDCFRIIKPSDQKFSAGGYNFRFNAKGLEVFGKPEMVDKM
                     RGILIAFTGTDQQKQEHLLKLAQSGKVEGI"
BASE COUNT         2377 a         2305 c         2382 g         2268 t
ORIGIN      
        1 aacctcagtg aatccggcct gaattcaggc cggatatcct tttacttcgt tttctcgcaa
       61 tccagaactg tatttacaat catctgatac cccgtgcagc gacaaagatt tcccgccagt
      121 ccgcgacgaa tttccgtaat ggttaatggc ttctcgcgtg gtttcgccag cattgccgtg
      181 gtagccataa tcaggccagg cgtacaaaac ccgcactgca ctgtgccgga tttcgcataa
      241 gcctgctgaa catgagaaag ttttccgcct ttcgcttcac cttccagcgt gcggatctct
      301 tttccttcag cccaggcggc aaggtataag caactgtcta ttgctgtgcc gtcgaccaac
      361 accgtacagg caccacattc acccacgcag cacccttgtt tgacacttag cagtccttgt
      421 tcgcggagta attccgagag cggcgtgcct ggtgcggcgt gaagctgaaa aggcatcccg
      481 ttaatggtgc attcggtggt aattgtttcg ctgtgattca ttgcaatttt ccccccgccg
      541 cggcgacggc ttcgctaatc acttttttgg tcatcgtctg gatgagatgc agacgaaact
      601 ctttactggc ccgccatgaa gaacgcgggg cgacatcttg caggacagat tcgctgatag
      661 cttccagcgt ttgcaggttt aatggcgcat tttgtgcagt ctgttcggca tgttggcagc
      721 gaatcggcgt tggcgcggca acaccaaatg ccaggcgtaa ttcgctgaaa ttgccgttat
      781 ccagtcggca atgtgcggcg cagccaatcg ttgaaatatc cattgcgtcg cgcatggcat
      841 atttaaaatg cgcgctgccc gcgtgttctt tcggctgtgg cggaaaatga aaggcgacga
      901 ggatttcgtc atgctcaaga gacactttgc ccggcccggt gtgaaagcca ttaatcggga
      961 cgaaacgaac accgcgcggg gagtggatct ccagtttcgc gtcataaatt agcgttggcg
     1021 tggcagaatc tgcgctggtg gcaccgttgc aaatatttcc accgtaggta gcgacgttac
     1081 ggatctgcgg tccagcaatg aacgtggccg cagcacataa cgccgggaga tgacgttgag
     1141 ttataggatc ttctattagc tgggtaaatg tcgttgcaga gccgatacgt agcgagccat
     1201 cttccgccag cgtaattccc cgcagctccg ccagattatg aatatcaaca atatggcgat
     1261 aacggtcatt gtgatggtgg agctgaatca gtacgtcagt gccaccggcg agcagtttgg
     1321 cctgcgggtt gtcagccagc aggttgatgg catcggcaag ggttgctgcg cgatggtaag
     1381 aagcaaaatc aaacatgatg ttatcctcaa atcaatcctg ccagatggaa ctcttcatat
     1441 aaccgttttg gcgtcagcgg cagtgtattg attgcaacac cggtagccat cttcaccgcg
     1501 ttacgaatag cagcggcaac aggaattatg ggtggctcac ccagtgactt atgtccgtat
     1561 gcggattgcg gctcattgat ttcgacgaac gcgctttcca gttgtggcag atccggcatg
     1621 gtcggcattt tgtaatccag cagattgggg ttacggacca cgccgctttt agcatcgatg
     1681 atcatctctt caaatagcgc ccagccaatg cccattccca ttccgccgtg tacctgacct
     1741 tctgccagca gtggattaag aatatgccct gaatcatgaa cgttgaggat gcggttgatg
     1801 gtgactttgc acagcgcaat atcgaccgtc agatcaacaa aggtacagcc aaacgccggt
     1861 gggttagtgg tggttttgat ggagctttca gcagagagct gcccgccgcg ttcagggtgg
     1921 tagaaagcgt ccatcgccaa atcttttaac gacattaacg gctcttccgg tcgttcaacc
     1981 agcacgatat ggccttttat cagggtcaaa ttcatcgctg actgatgtag catgactgcg
     2041 gcgtgagcga tgattttctc ttttaataat agtgccgcac tgcgcagcgc aggcgcggca
     2101 acatagctct ggcgtgaggc aaatgcgccg ggatcgaacg gcgtaacgtc ggtatcttgt
     2161 gttgaaataa cgcgaacgtc gctgaccgga acccccacgg tttctgccac catttgcgag
     2221 aagacggtgt cggcaccctg accgatttcc gtcgcgccgc tttgcacgtt gatggttcca
     2281 tcctgattca tcagaaggcg cgcgcctgct atttctacgc cgacaggcca ggtgttagag
     2341 gtgtagctaa aacaggcgac gccaacgccg cggcgcaaat tgccttgctg gttctggcat
     2401 tctgcacggc gtttttccca ttcaaagatt ttccggcctt tttcaagaca ctccggcaac
     2461 cctgcgctgc aaatacgttt gcccgtgagc ggattagcat ctccttcgcg ggcggcgttg
     2521 cgtaaacgaa tttcaacagg atcaatacct aacgctgtcg cggcgtcatc aagcatagac
     2581 tcaacggcaa atacgacttg tggcgcgcca taaccacgca tcgcaccagc cgaggggagg
     2641 ttggtatagc aggtctttga actgtaagcg taggcacaac gaggataaag gtaagcgact
     2701 ttattccccc ccgcagaagc gatggagtgc ccgtgagatg cataagcgcc ggtgttagac
     2761 agaacatcca gactataacc tttcaaggtt ccgtcgcggt tcacgcccat ttgcccgtca
     2821 atagtaaaag cgtggcgggt acgggttgcg aggaaacact cttcacggct aagggaaact
     2881 ttcaccggaa tgccgccaag cttgctggtc aggaatgccg ccattggctc ttccagtaca
     2941 tcctgtttat taccaaaacc gccaccgaca aacggtttga tgactcgtac gcatgaccag
     3001 ggaatatcca gcgcctgacc aaccacgcgg cgaacaatgt gcgggatctg ggtgctggaa
     3061 acgatggtaa ttcgcgagtc atcctccatc cacgccagcg atgttacgct ttccatgtga
     3121 caatgttgaa taacgggagt ctgatagtgc ccctgtacct ggtagtcggc ggcatcgatt
     3181 gtttgttgga cattgcccgt cgacatcgtg ctttgtttca gtaaattgcc gccgttatgg
     3241 attggtgcag cgtcttctgt cagcgccgct tctggcgtgg tgataacggg taattcttgc
     3301 cactcaatgc tgaccaattg cgccgctttt tctgccgtga gttcatcgcg ggccacgacg
     3361 atggcaacgg cgtcaccatg atgacgaaca tggcgagtta gcagtgcgcg atcggcggta
     3421 tcgcgcttgt tttcgtcaag tgtctaggca tgcccagctg tagcgaatgg aatatcaggc
     3481 acatcttccc aagtaaaaat cgccagtacg cccggtaaac ttctggcttg ttcatcatta
     3541 atacttacgg cataaccatg tgcgataggg ctacgtacat atttcgcgta acacatgccc
     3601 gccataacat aatcgtcagt atatcgtgcc cgcccggtga ccttagcaat ggcatcgacg
     3661 cgcatgcatg attcacccgt agcggttgct tcccgcgctt ccatagaaat cccctcgatt
     3721 tgaacatttg gttcttatgg caaagattta gtctaagcag gcaagattta cgccagaaac
     3781 gacgataagc gttaggggag gaaggaagtg tgatgacgtt aaaaaaacac agatatgata
     3841 cgaaacagga ctctgaatag ggacagtgat acgtatcaat atgagtggcg ataaaatcaa
     3901 tgtgataaat caggattatt acgcatcagg atgggaaata gggtaaaaag tcaggaactt
     3961 cgatacctct acagcgaata ttttgctgat gatacttccc tttgccaaat gaaattaacc
     4021 tctttctcta ttaccacgtt tttccagaag caagagattg ggtatcaaga ggctggctgc
     4081 tatgatatgg cgtcttgttt tttaagcgag gaaagatttt gagtgcggga cgcctgaata
     4141 aaaaatctct gggtatcgtg atgtttttat cggttggact gctattggcg ggctgttcgg
     4201 gtagcaaatc atccgataca ggaacgtatt ccggctccgt ttacaccgtg aaacgggggg
     4261 atacgctata tcgtatttcg cgcaccacgg gaaccagcgt aaaagagctg gcgcgactga
     4321 acggcatttc ccccccttac accattgaag ttggtcagaa actaaaactg ggtggggcga
     4381 aaagtagcag tagtacacgt aaatcaaccg ccaaatcaac gactaaaacc gcatcggtta
     4441 caccgtcatc agcggtaccg aaatcttcct ggccgccagt agggcaacgt tgttggttat
     4501 ggccaacgac agggaaagtt atcatgccgt attcgacagc agatggcggc aataaaggga
     4561 ttgatatctc agctccacgg ggtacaccta tttacgccgc gggtgcagga aaggtggtgt
     4621 atgtgggcaa ccagctgcgt ggctacggta atctcatcat gattaaacac agtgaagatt
     4681 acattacggc ttacgcccat aatgacacga tgctggtaaa taatgggcaa agcgtgaagg
     4741 ctgggcaaaa aatcgccact atggggagca cggatgcggc atctgttcgc ctgcatttcc
     4801 agattcgtta ccgtgcaacg gcaattgatc cgctacgtta cttgccgccg cagggcagca
     4861 agccaaaatg ctgatggcga attaatcagc agtcagcagc gcggcacttg ctaaggagag
     4921 cgtaaggttt ataatgcctt acgcatctcg aagcgggcgt agttcaatgg tagaacgaga
     4981 gcttcccaag ctctatacga gggttcgatt cccttcgccc gctccatgac acttcttaaa
     5041 gtctactaac ttactgaatc ctaagggata cggcttaacg ccttccgata gttttactca
     5101 ctgtaaccaa cactgtgact aactttgttg aagtagtgag cagacctaag gtctcagtac
     5161 atgctcaagt caagaaccta cctttatcag cgtaatggtg tcttttacat ccgtcttagg
     5221 atgaagacca ctagtcgcct gaccgcttcg cttccttctc acaaccgtta taaactagcg
     5281 tcagtatctt tacggaccaa ggatagacgt accgctatgg ctcactcacg gcacatcaag
     5341 tcagcactta aagcaatcca tgcagacaac cctaacgcct cttatgagga gctacgggag
     5401 cacctgaaga ccattgtcga gtgggaactt agtgtcagcc gtgatgacct gaacgacccg
     5461 gagtcctacc agctttacgt tgaccagtac gatgacatta agtccaacct tcgggaagct
     5521 gtagcgaccg agcgtctgac tgtagaccag catcgctata ttaatgacgt tatcggtgtg
     5581 cttaaggcct gtcaggatag actcaatggc gatagctctg gcctattgtc ttaccttgag
     5641 cctgagactg gcagtctaag accgtccgtc tccctatctg tattggcgga acctgaagtc
     5701 cctgaaccta aagcactgac cctcgcgtct ctcattgagc agtacgagca ggagaacgcc
     5761 cagaactgga agccagcgac cctaagcgaa aaccgagcgt cacactctac gctaatcgag
     5821 atattcgact atctggatat tcaggatgtc ggcaaggcga cccgtgcaga tatgctcaga
     5881 gttcgtgaag tcctccagca gctaccgaag aaccgcaagc aacgctttaa gtctatgccg
     5941 ctgtctgact tgctgaaccg ggagtctaag actgactgtc tggacgtcgt taccattaac
     6001 aataaatatc ttatcaagat ggctgctgtg tttaagtggg cggtacgcaa cgacttgata
     6061 gctaagaacc tgaccgaagg tcttgagctg aaagtccctc agcgtaaagc ctccgacgct
     6121 cgtgatgcgt tcagtcctga gcaggtaggg caactactgg tcgcagctaa agcgtactcg
     6181 cagaagacct caggtaagcc ataccattat tacgtcaccg ctctggctgc gattacaggt
     6241 gcgagactta acgaggtggc ccaacttcag gttaaagatg tcaggaccac tgaggctggg
     6301 accgttttta tccatatcaa cgaagacgat agcagcctgc cgggtaagag tatcaagaac
     6361 gcccacagtg accgctgtgt cccgttggta gatggtgctt atggttttgt attggctgac
     6421 tttatgtcac tcgtggaaga ccgtagaaag actgagggtg ataatgctat ggtgttcaat
     6481 ggcctcaagc taatgaagaa cggctacggt gagcaggtta gcaagtggtt taaccgaacg
     6541 ttgctgccca aagtcctcgc tgaccgtagc ggcttagctt ttcactcgtt tagacataca
     6601 gttgcgaccc agttaaaaca acatggggtc gagttagcgt atgctcaggc gattatgggt
     6661 catagctcag ggtctataac ttacgaccgt tacgcgaaag aggtcgaggt ggaaacgctg
     6721 aaagaaaaat tagcggagtc attgtcagta aaaaaaatag acggaaagta atcgcatagg
     6781 gatagcggaa tgtgaggacg taatggtcaa agaattttta gttagcaaag aaaataagcc
     6841 ttcatatgaa tgtatgaata ctgctgaaac ttgtttgggg ctggctggaa tggcttcgga
     6901 agattggcaa cgtgagcatc attgccgcac aggtataata tttactgctt ttgctattga
     6961 ggcaatgttc atcttctata gaaaacaggt tgaccccggc tacgataaga cgcagaaaga
     7021 gtgcagaaag acaatgcaca agaatacgtt aaaattgtgc ggtataaaca attatatggg
     7081 gacaaaacct taccaaatta tcaaagagtg tcttgaggta agagacgcta ttgctcacgg
     7141 cgattcgtat acttcgagct ttaatttctc ggcggatcat ttggataacc aagacgatat
     7201 tgcaaaagtg tcctggcggt aaactctgaa caatttcggg gattaactgt tgagcgcctt
     7261 gcaaaataca ttgaaatggc aaagcatatt gaccatgaga tttctgataa tggttacagg
     7321 ccttctgaag gttaccttcc agcagccgag cgcagaagtc tgatgttagc ttttggcgtc
     7381 agtggtgtgt caatgtggcc cagtgcttga gcgataatac acctcactat agaggaatgg
     7441 acccttaact atcactatag gggagagacc ctaagtcccc accttaatag cagtcacgac
     7501 ccatacacac attcattaag tctcaggctt ccggtagcgg accgattgga aaccatccgc
     7561 agacgcctag tgttaaatca ccttggcctg agaccattcc ttttaacgta agctatcttc
     7621 ccttttgaca ggcggggagc agtagaccac atccgacttt aagttggctc tgtgataatc
     7681 gctgcgcgga taactgaaga accaagacac cattactgag gtctgccatc catttgtgga
     7741 caggcggaaa ctcaaagagg ttagatcagt ggtgtctgag gttggagctt tgagtcttga
     7801 ccagcaggct ggaggagacc atgagtttat cttgttatgg attgtgttat cacaattcca
     7861 ataagaagaa ctttaagtcc agcctccact cccgccaacc cgaagtaaaa ttatggtcgc
     7921 tatgatgtgg tctccatgct tcgggtctta ctggaagtac tgaacacttt cgcgtcaagt
     7981 tctccgaagt tcattcctgt ttgactaaac tatcactata gggaccatat ctcgcctctg
     8041 acctcctgag agacggcaag cgagaaaccg tggtccacgc tagcaagcta gccaccagcc
     8101 taaggcaaga cagcctctgg tcagcctgtg tgttctttgg tctcgtgact taagtctatc
     8161 accacacacg ctcggacatg ctgtcgaacc ttcatctcta tggtcatcgc agaccactaa
     8221 ccccaattac ctcaagtcat tctgtttgcg tcctgttgcc tgctggtctc cctttacgtg
     8281 ggggcttgag taggtcagtg gggtgcgact tcagggtgca aacaaaagga gtacacgtaa
     8341 atgtcccaac aagaaatcag catcattaac ctcgaccagc ttgtgtccat gacctcagtt
     8401 gaaattgctg agctgacagg aaaagaacat aaacacgttt tacgagatat tcgtaatatg
     8461 gtcgaagagc ttaacggggc caaaactgag cactgctcaa ctttgagcag cgagcttaac
     8521 gggtccaaat ttgggctcgt tggcgaggaa gtatacaagg acgcgaaagg tgagagccga
     8581 acaatgtatc gtctcgaccg taagcatact ttcattctgg tcgctggcta ttccgttcac
     8641 ctccgcgcca agtgttacga ccatattcag acgttagagc gtcgagtcct tcaactggaa
     8701 gaccagaaga agcgagcagc cattcagtcc gctaaccgcc gaggcgtgac ttggggcgac
     8761 tactgcaaaa cctatggtct gcccgctcag aagctaatga ccgcacttct ccagcatcgt
     8821 ggcctctttc gtaagaaccc aatcagtaac gagtggtctg taaatccgaa gtattccgac
     8881 tgctttcgta tcattaagcc gtcagaccag aagttctcag caggtgggta taacttccgg
     8941 ttcaacgcta aggggctgga ggtcttcggt aagcctgaga tggttgacaa gatgcgaggc
     9001 atccttattg cattcactgg caccgaccag cagaagcaag agcacctcct gaagctggca
     9061 caatccggta aggtggaggg aatctaagat gatgattgac ccgcgcacac ctgaggggcg
     9121 catgactctc cgttaccgtg gctaccggac tgaagtcctc ctcagagagc ttggtctaga
     9181 cccggaagat gagacccgcc agcaccaatc ccgagacgag ctaatagccc aactggtcgc
     9241 tatgaaactg ccgcttaacc gttagtagct cacctactca atacctaatt taaacacctt
     9301 ttgagatgac cgatgcgggg agacccgtag gc
//
LOCUS       DAJTRC010000133         9301 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.133, whole genome shotgun sequence.
ACCESSION   DAJTRC010000133 DAJTRC010000000
VERSION     DAJTRC010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9301)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9301)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9301
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.133"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            28..879
                     /gene="hchA"
                     /locus_tag="OO357_003558"
     CDS             28..879
                     /gene="hchA"
                     /locus_tag="OO357_003558"
                     /EC_number="3.5.1.124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein deglycase HchA"
                     /protein_id="HCR5888373.1"
                     /translation="MTVQTSKNPQVDIAEDNAFFPSEYSLSQYTSPVSDLDGVDYPKP
                     YRGKHKILVIAADERYLPTDNGKLFSTGNHPIETLLPLYHLHAAGFEFEVATISGLMT
                     KFEYWAMPHKDEKVMPFFEQHKSLFRNPKKLADVVASLNADSEYAAIFVPGGHGALIG
                     LPESQDVAAALQWAIKNDRFVISLCHGPAAFLALRHSDNPLNGYSICAFPDAADKQTP
                     DIGYMPGHLTWYFGEELKKMGMNIINDDITGRVHKDRKRLTGDSPFAANALGKLAAQE
                     MLAAYAS"
     gene            complement(987..2345)
                     /gene="hprS"
                     /locus_tag="OO357_003559"
     CDS             complement(987..2345)
                     /gene="hprS"
                     /locus_tag="OO357_003559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine kinase
                     HprS"
                     /protein_id="HCR5888374.1"
                     /translation="MKRLSITVRLTLLFIFLQSVAGAGIVWTLYNGLASELKWRDDTT
                     LINRTAQIKQLLIDGVNPDTLPVYFNRMMDVSQDILIIHSDGINKIVNRTNVSDDMLN
                     NIPASETISAAGIYRSIINDTEIDALRINIDEVSPSLTVTVAKLASARHNMLEQYKIN
                     SIIICIVAIILCSVLSPLLIRTGLREIKKLSGVTEALNYNDSRVPVEVNALPRELKPL
                     GQALNKMHHALVKDFERLSQFADDLAHELRTPINALLGQNQVTLSQIRSIAEYQKTIA
                     GNIEELENISRLTENILFLARADKNNVLVKLDSLSLNKEVENLLDYLEYLSDEKEICF
                     KVKCNQQIFADKILLQRMLSNLIVNAIRYSPEKSRIHITSFLDANGSLNIDIASPGTK
                     INEPEKLFRRFWRGDNSRHSVGQGLGLSLVKAIAELHGGSATYHYLSKHNVFRITLPQ
                     RN"
     gene            complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="OO357_003560"
     CDS             complement(2345..3016)
                     /gene="hprR"
                     /locus_tag="OO357_003560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor HprR"
                     /protein_id="HCR5888375.1"
                     /translation="MKILLIEDNQRTQEWVTQGLSEAGYVIDAVSDGRDGLYLALKDD
                     YALIILDIMLPGMDGWQILQTLRTAKQTPVICLTARDSVDDRVRGLDSGANDYLVKPF
                     SFSELLARVRAQLRQHHALNSTLEISGLKMDSVSQSVSRDNISITLTRKEFQLLWLLA
                     SRAGEIIPRTVIASEIWGINFDSDTNTVDVAIRRLRAKVDDPFPEKLIATIRGMGYSF
                     VAVKK"
     gene            3149..3562
                     /gene="hiuH"
                     /locus_tag="OO357_003561"
     CDS             3149..3562
                     /gene="hiuH"
                     /locus_tag="OO357_003561"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HCR5888376.1"
                     /translation="MLKRYLVLSVATAAFSLPSLVYAAQQNILSVHILNQQTGKPAAD
                     VTVTLEKKADNGWLQLNTAKTDKDGRIKALWPEQTATTGDYRVVFKTGDYFKKQNLES
                     FFPEIPVEFHINKVNEHYHVPLLLSQYGYSTYRGS"
     gene            3671..4675
                     /gene="msrP"
                     /locus_tag="OO357_003562"
                     /pseudo
     CDS             3671..4675
                     /gene="msrP"
                     /locus_tag="OO357_003562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310736.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase catalytic
                     subunit MsrP"
     gene            4676..5311
                     /gene="msrQ"
                     /locus_tag="OO357_003563"
     CDS             4676..5311
                     /gene="msrQ"
                     /locus_tag="OO357_003563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-methionine-sulfoxide reductase
                     heme-binding subunit MsrQ"
                     /protein_id="HCR5888377.1"
                     /translation="MRLTAKQVIWLKVCLHLAGLLPFLWLVWAINHGGLGADPVKDIQ
                     HFTGRTALKFLLAALLITPLARYAKQPLLIRTRRLLGLWCFAWATLHLTSYALLELGV
                     NNLALLGKELITRPYLTLGIISWVILLALAFTSTQSMQRKLGKHWQQLHNFVYLVAIL
                     APIHYLWSVKIISPQPLIYAGLAVLLLALRYKKLLSLFNRLRKQAHNKLSL"
     gene            5569..6219
                     /gene="zinT"
                     /locus_tag="OO357_003564"
     CDS             5569..6219
                     /gene="zinT"
                     /locus_tag="OO357_003564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-binding protein ZinT"
                     /protein_id="HCR5888378.1"
                     /translation="MAIRLHKLAVALGVFIVSAPAFSHGHHSHGKPLTEVEQKAANGV
                     FDDTNVQNRTLSDWDGVWQSVYPLLQSGKLDPVFQKKADADKTKTFAEIKDYYRKGYP
                     TDIEMIGIEDGIVEFHRNNETTSCKYDYDGYKILTYKSGKKGVRYLFECKDPESKAPK
                     YIQFSDHIIAPRKSSHFHIFMGNDSQQSLLNEMENWPTYYPYQLSSEEVVEEMMSH"
     gene            complement(7297..7566)
                     /locus_tag="OO357_003565"
     CDS             complement(7297..7566)
                     /locus_tag="OO357_003565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5888379.1"
                     /translation="MLPQHSDIEIAWYASIQQEPNGWKTVTTQFYIQGFSEHIAPLQD
                     AVDLEIATEEENSLLEAWKKYRVLLNRVDTSTAPDIEWPEEPDTM"
     gene            7623..8291
                     /locus_tag="OO357_003566"
     CDS             7623..8291
                     /locus_tag="OO357_003566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HCR5888380.1"
                     /translation="MVKLMKKNTDDGAKIYTPLTLKLYDWWVLGVSNRLAWGCPTKEH
                     LLPHFLEHLGNNHLDIGVGTGFYLTHVPESSLISLMDLNEASLNAASTRAGESKIKHK
                     ISHDVFEPYPAALHGQFDSISMFYLLHCLPGNISTKSCVIRNAAQALTDDGTLYGATI
                     LGDGVVHNSFGQKLMRIYNQKGIFSNTKDSEEGLTHILSEHFENVKTKVQGTVVMFSA
                     SGKK"
     gene            8607..>9301
                     /locus_tag="OO357_003567"
     CDS             8607..>9301
                     /locus_tag="OO357_003567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005105549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5888381.1"
                     /translation="MLPVNNPPLSTGNVSFYRTTSIDNVHNNYLSEWVEWTKNSISGE
                     NRETAFTRLQLCLENSETSLDLSCLGLRSLPRLPDNLDEINVSNNQLSMLPELPRALK
                     ELNASSNQLSALPELPVSLEYINVSDNHLFALPELPASLEYINVSDNHLSVLPRLPMS
                     LELLDAARNALEVIPDFPERDDHIIRIFWLNQNRITAIPESILGLSSDSVVNLRENQL
                     SPRIMQTLLQQTA"
BASE COUNT         2673 a         2094 c         1898 g         2636 t
ORIGIN      
        1 taactaagca acaataagga atacactatg actgttcaaa caagtaaaaa cccgcaggtc
       61 gatattgctg aagataatgc gttcttccct tcagaatatt cgcttagcca atatacgagt
      121 cccgtctctg atcttgatgg cgtggactat ccaaaaccgt atcgcggtaa acataaaatt
      181 ctggtgatcg ccgcggacga acgttatttg ccgaccgata acggaaaact gttctcgacc
      241 ggtaaccatc cgattgaaac gttgctgccg ttgtatcatc tccatgctgc aggtttcgaa
      301 ttcgaagtgg cgaccatttc cggtctgatg accaagtttg aatactgggc tatgccgcac
      361 aaagatgaaa aagtaatgcc attctttgaa cagcataaat cgttattccg taatccgaag
      421 aaactcgcgg atgttgtcgc cagcctcaac gctgatagcg aatatgcagc aatctttgtt
      481 cctggtgggc atggcgcact tattggttta ccagaaagcc aggacgtggc tgccgcttta
      541 cagtgggcaa tcaaaaatga ccgttttgtt atctcccttt gccacggtcc ggcggctttt
      601 ctggcacttc gccacagcga taacccactg aatggttatt ctatttgcgc attcccagac
      661 gccgcagaca aacaaacgcc agatattggc tatatgccgg gtcatctcac ctggtacttc
      721 ggcgaagagt tgaagaaaat gggcatgaac atcattaatg acgacatcac cgggcgagta
      781 cataaggacc gtaaacgtct caccggcgac agtccttttg cagcgaatgc gttgggtaaa
      841 ctggcggcgc aggaaatgct ggcagcttac gcgagttaat ccctgttaca ttgtgatgta
      901 agcgcataat caatcgcagc cacgttaata ctcacgacgt ggctgcgttt tttcacggtt
      961 aacttatggc gtactgaagc ccgatgttaa tttctttgcg gtaacgtaat tcggaacacg
     1021 ttatgcttac tgagatagtg atacgtagca cttcctccat gtaattcggc aatcgctttg
     1081 actaaagaaa ggccaagtcc ctgacctacg gaatgacgcg aattatctcc ccgccaaaat
     1141 ctacggaaga gtttttcagg ctcattaatt ttcgttccag gactggcgat atcaatatta
     1201 agagagccgt tggcatcaag aaaactggtt atatgaatac gcgatttttc tggtgaatat
     1261 cgaatggcgt taacaataag attcgataac attcgttgca gtaaaatttt atccgcaaag
     1321 atttgttgat tgcacttgac cttaaagcaa atctctttct cgtctgaaag atattcaaga
     1381 tagtccaaaa gattttcaac ttccttattg agagaaagcg aatccagttt caccaaaaca
     1441 ttgtttttgt ctgccctggc aagaaacagt atattctctg ttaaccgcga aatattttcc
     1501 agctcttcaa tgtttccggc aattgttttt tgatattcag cgatacttct gatttgactg
     1561 agcgtaacct gattctgacc cagtaatgca ttaattggcg ttctaagttc atgagcgaga
     1621 tcgtcagcaa attgactaag gcgctcaaaa tctttgacta aagcatgatg cattttattc
     1681 aacgcctgcc caagaggttt tagttctctc ggtaatgcgt taacctcaac cggcactcgg
     1741 ctatcgttat aattcagcgc ttccgttacg ccactcaact ttttgatctc tcgcaacccc
     1801 gtcctgatta acaacggact taatactgag cacagtataa tggcgacaat gcaaattata
     1861 atgctattaa ttttatactg ttcaagcata ttatgtctgg ctgaagccaa tttagccaca
     1921 gtaaccgtta atgatggcga aacttcatca atgttaattc gtaaagcatc tatctctgta
     1981 tcattaataa tgcttctgta aatgcctgct gcgctgattg tctcactagc aggtatgtta
     2041 tttaacatgt catcactgac atttgtccgg ttaacaattt tattgatgcc atcactatga
     2101 atgatcaaaa tatcctgact aacatccatc atccggttaa agtacacagg taacgtatct
     2161 ggatttaccc catcaattaa caactgcttg atctgcgctg tccggttaat gagtgttgta
     2221 tcatcgcgcc atttcaactc acttgccagg ccattataga gagtccatac aattccagcg
     2281 ccagcaacag actgcagaaa tataaaaagc aaggttaaac ggacggttat agataatctt
     2341 ttcattattt ttttaccgct acgaatgaat agcccatccc ccggattgtg gcgattagct
     2401 tttcaggaaa aggatcatca acttttgcgc ggagcctgcg aatggcgaca tccaccgtat
     2461 tggtatcact atcaaagttg attccccaaa tttcactcgc aataaccgtt ctgggtataa
     2521 tctcgccagc tctggaggcc agtagccaaa gtaactgaaa ctccttgcgc gtcagtgtaa
     2581 tactgatatt gtccctgctg acactttgac tgacagagtc catttttaag ccactgattt
     2641 ctaatgttga attcagagcg tgatgttgcc ttaattgtgc ccgaaccctt gctagcaact
     2701 cagaaaatga aaaaggtttt accagataat catttgcccc actgtccaac cctctgactc
     2761 tgtcatcgac agaatccctt gcagtaaggc aaataacagg ggtttgcttt gccgttctta
     2821 acgtttgtaa gatctgccag ccatccatac ccggaagcat aatatccaga atgatcaatg
     2881 cataatcatc cttcagcgca agataaagcc catctctgcc atcagaaacg gcatcaatga
     2941 cataacccgc ttcggaaagc ccctgcgtta cccattcctg ggtcctttga ttatcttcaa
     3001 taagtagaat cttcatgaaa ataatatgcc ataaatcata tgttatagaa acaacctggt
     3061 tcattacaaa attgtaatgc tgctgtaagg ttaccctgga cgttttttcg ctatcctcaa
     3121 aactcattca catgacaagg atataaacat gttaaagcgt tatttagtac tctcggtagc
     3181 aacggcagcc ttttcattac cttctttggt ttatgccgca caacaaaaca ttcttagcgt
     3241 gcacattttg aaccagcaaa caggaaaacc cgctgccgac gtgacagtca ctcttgaaaa
     3301 gaaagcggat aacggctggt tacaacttaa taccgccaaa acagataagg atggacgaat
     3361 taaggcactg tggcccgagc aaactgcgac tactggcgat taccgtgtcg tatttaaaac
     3421 cggggactat ttcaagaaac aaaatcttga aagtttcttc cctgagattc ccgttgaatt
     3481 tcatattaat aaagtgaacg agcattatca tgtgcctttg ttactcagcc aatatgggta
     3541 ttcaacctat cgtggcagtt aatgcgtaaa tatgtaaaag ctgatctctg ctactgttag
     3601 ggaggtgact ccctgacaga ttaacagtaa acagctcttg ttggttaacg acaaaaaagt
     3661 gtgatggctt atgaaaaaga agcaattttt aaaagaatca gatgttacgg ccgagtcggt
     3721 attctttatg aagcgtcggc aggtgttaaa agcactgggc atcagcgcag ctgcattttc
     3781 tttgcctcac gctgcgcatg ccgatctgct tagctggttt aaagggaacg atcgtccgcc
     3841 cgcccccgcc ggaaaaccgc tggagttcag caagcctgcc gcctagcaaa ataacctgcc
     3901 actgacgcca gcagataaag tctccggtta taacaacttc tatgaattcg ggctggataa
     3961 agacgatccc gcggctaatg ctggtagcct gaaaaccgat ccatggacac tgaaaatcag
     4021 cggcgaagtg gcaaaaccat tgaccctcga tcatgatgat ttaacccgtc gcttcccgct
     4081 ggaagagcgt atttatcgta tgcgctgcgt ggaagcgtgg tcgatggtgg tgccgtggat
     4141 tggttttccg ctacacaaat tgctggcgct tgccgaaccc accagcaatg cgaagtatgt
     4201 cgctttcgaa acgatttatg cgccggaaca gatgcctggc cagcaggacc gctttatcgg
     4261 cggcgggctg aaatatcctt atgtcgaagg attgcgtctc gacgaagcaa tgcatccgct
     4321 cacactgatg accgtaggtg tttatggcaa ggcgttacca ccgcaaaatg gcgcgccggt
     4381 gcgactgatt gtgccgtgga aatatggctt taaagggatt aaatcgatcg tcagtattaa
     4441 gctgacccgc gagcgtccgc caaccacctg gaatctggca gcgcctgacg aatacggttt
     4501 ttacgccaac gttaatccgc atgttgatca cccgcgctgg tcacaggcta ccgaacgatt
     4561 tattggttca ggcggcattc tcgatgttca gcgccagcca acactactgt ttaatggtta
     4621 cgccgaccag gtggcatcgc tgtatcgtgg cctggatttg cgggagaatt tctaaatgcg
     4681 tctgacagca aaacaagtga tatggctgaa agtttgcctg catcttgccg gattgttgcc
     4741 gtttctctgg ctggtctggg cgatcaatca cggtggactg ggtgccgatc cggtgaaaga
     4801 tattcagcat tttaccggtc gcactgcact gaaatttttg cttgcggcct tgttaatcac
     4861 acctctggca cgctacgcaa aacagccgtt attgatacgc actcgccgcc tgttaggctt
     4921 atggtgcttt gcctgggcga cactgcattt aaccagttac gcattgctgg agttgggcgt
     4981 gaataatctg gcgttattag gtaaggagtt aattacccgg ccttatttaa cgttaggaat
     5041 tatcagctgg gttattttgc ttgctttagc gttcacttca acccagtcga tgcagcgaaa
     5101 actcggcaaa cactggcaac agttgcataa cttcgtctat cttgtcgcga tcctggcccc
     5161 aatacattat ctgtggtcag tgaagattat ttcaccgcag ccactcatct acgccgggct
     5221 ggctgtactg cttttagcct tacggtataa gaagttgctt tcccttttca accggttacg
     5281 caagcaggcc cacaacaaat tatctctata acttcatatt gccgacaaac ttcgcgttat
     5341 cccggccgga tgcggcgcga gcgccctatc cggcccacac agatcgcatc aagttatgtt
     5401 tcgtttgtca tcaacctttc atcaccagaa taatttattt tatttcaata agatagttca
     5461 atacacctga gaataccatg ctctcatttc ctaatgagtt gttgcatttt ttcatatgtt
     5521 acaatataac attacacata atgtacatta actctggagg aaattgtttt ggcgattcgt
     5581 cttcataaac tggctgttgc tttaggtgtc tttattgtta gcgctcctgc cttttcgcat
     5641 ggtcatcatt ctcatggcaa acccttaaca gaggtcgaac aaaaagctgc taatggtgtt
     5701 tttgatgata ccaatgtaca aaaccgaacg ctcagtgact gggatggagt ctggcaatcc
     5761 gtttaccctt tactgcaaag tggcaaactt gatcccgtct ttcagaagaa agcggatgca
     5821 gataaaacca aaacatttgc tgaaattaaa gattattatc gcaaaggtta tccgacagat
     5881 atcgaaatga ttggcattga ggacggcatt gtcgaattcc atagaaataa tgaaacaaca
     5941 tcctgtaaat atgattacga tggatacaaa atactcacct ataaatcagg caagaaaggc
     6001 gttcgttact tattcgaatg taaagatcct gaaagcaaag cccctaaata tatacaattt
     6061 agcgatcata taattgcacc acgaaaatca tctcattttc acatttttat gggtaatgat
     6121 tcccagcaat ctttattgaa tgaaatggaa aactggccaa cgtattatcc ctatcagttg
     6181 agtagcgaag aagtggtcga ggagatgatg tctcattgat ttttttgcgc tgacttcagt
     6241 tatttaacag aacagtgcct ggtagtaaca acaataattt accaagacaa ataagtaaga
     6301 caaactcaga cacgctcata cttgagtcgt gtctttttta ttatgtctgt agaaaaaaac
     6361 tgagaaaaag gctaacaata aatacacaac tcagccatac agactggcag cgcaaatgtt
     6421 tccaggagca agtcatctgt catggtaaag ctaaccagaa atataggtta attttggtgt
     6481 ttaaaccttt tttacttgtt agtatgatac aaaggctttc aaaaaagctg ggaaattcaa
     6541 caactcttgg aaattatgga ctttgtcccg cggctttaat ctgacggccg cgttcctttt
     6601 ttggctcaat tattgacccc ttctctacgc aacttcagtt cccgccagca actttgcggc
     6661 agtttcatag gatcaatgtc taaaagaata atggtgaccg ataagaaaac gactgaataa
     6721 ctgtagattt tcgctcgaaa ccttcctgtc agctccatag cgaatcaagc gctgaatgtc
     6781 acagtatcga acagaaaaca gtgacgatct aacccttcaa gaatattcta cgattgttct
     6841 gtttaggaaa agcaaggcgg gaagtcggga gataagtcat tgataaaatg gcggagagag
     6901 ggggatttga acccccggta gagttgcccc tactccggtt ttcgagaccg atgatacaga
     6961 gtcgaaaaat caaagagtta aaaatttttc ttggaaaaaa tgtcttaaaa tcgtacacga
     7021 aaaatcaatg aattacataa cattaaagaa gaatattcca agccttcttg agtccaccac
     7081 ctgttttcag gtgtcattca gacgtgattt gccccagatt gggaaagcac gcttttttct
     7141 cagcttattc cgttttgcct gtgtgttgaa taattccact gcagttatat aaaatcggta
     7201 acggctggaa atcattcaat actcgcacta tcgaaagttt accagccaac cgcagcacgt
     7261 cctgcatacg tcgtgtctgc ggtttttctt tttcgcttac attgtgtctg gttcttccgg
     7321 ccactcaata tcaggtgcag ttgatgtatc aacacggttc agcaacaccc gatacttctt
     7381 ccaggcttcc agcaacgagt tttcttcctc cgttgcgatc tccagatcta cagcatcctg
     7441 aagtggcgca atatgctcac tgaatccctg gatgtagaac tgtgtggtga cggtcttcca
     7501 gccattcggc tcctgctgta tcgaagcata ccaggctatt tcaatatcgc tatgctgcgg
     7561 cagcatttaa ccccttgtaa ttcatcgcca taattgattt aattcacaaa taaaactata
     7621 atatggtgaa attaatgaaa aaaaacacag atgatggggc taaaatttac acaccactta
     7681 ccctaaagct ttatgactgg tgggttttgg gagtatcaaa tcggcttgca tggggatgtc
     7741 ctacaaagga acaccttctt ccacactttc tggaacattt aggtaacaac catctggata
     7801 ttggtgttgg aactgggttt taccttactc acgtacctga gagtagtctg atatctttaa
     7861 tggatttgaa cgaagctagc ctgaacgcgg catctacaag ggctggggaa tcaaaaatta
     7921 aacataaaat tagccatgat gtttttgaac cttatcctgc ggcgttacat ggtcaatttg
     7981 attccatttc catgttttac cttcttcact gcctgcctgg aaatatatct acaaaaagct
     8041 gtgtaatacg caatgcggcg caggccttaa ctgatgatgg aactctatac ggagccacaa
     8101 ttcttggcga tggagttgtg cacaatagct tcggtcaaaa actgatgcgc atttacaatc
     8161 agaaaggcat cttttcaaac acaaaagatt ccgaagaagg cttaacacat atactctcag
     8221 agcatttcga gaatgttaaa accaaggttc aaggtactgt agtaatgttt tccgcttcag
     8281 ggaaaaaata gcatccaacc gcagcacgtt cttgcttaag acgtgctacg gtttccttta
     8341 cgatttgggg ttgcgacttt acctctattg ataatgcatt ccggcagaac gttcaaatat
     8401 gaacgtacga tatttaacta accgaaaaac aaaatatagc acaggcgaga catttaccat
     8461 ctaagaaaat agtatcgttt ttttacagtt aaatcaacat cacttcctta aaatgaaaac
     8521 aataaatttc aatcctgaaa cattttattt cagcttatta ttccccagta tcacatttta
     8581 tcaggatatc cttctgcgag gttattatgc ttcctgtaaa taatcccccc ctatccactg
     8641 gaaacgtctc tttttacaga actacatcaa tcgacaatgt tcacaataat tatctctccg
     8701 aatgggttga atggactaaa aacagcattt ccggagaaaa cagggaaact gcttttaccc
     8761 ggctccaatt atgtctggag aacagtgaaa catcgttgga cttatcttgt ttaggtctca
     8821 gatctctacc acgattgcct gacaatcttg atgaaattaa tgtaagcaat aaccaactat
     8881 caatgctccc cgagctacca agggcattga aagagctgaa tgcaagcagt aatcaattat
     8941 ctgcacttcc tgaattacca gtgtcgctgg aatatataaa tgtgagtgat aaccatttgt
     9001 tcgcacttcc tgaattacct gcgtcactag aatatattaa tgtaagtgac aatcacctgt
     9061 ctgtacttcc gaggttacca atgtcattgg aattacttga tgcagccaga aatgctttgg
     9121 aagtaatacc agattttcca gaaagagatg atcatattat aagaatattc tggcttaatc
     9181 agaaccggat cacggcaatt ccggaaagca tacttggcct cagttctgat agcgttgtca
     9241 atcttagaga aaatcaacta tctcccagaa taatgcaaac tttgttacaa caaaccgccc
     9301 a
//
LOCUS       DAJTRC010000134         9242 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.134, whole genome shotgun sequence.
ACCESSION   DAJTRC010000134 DAJTRC010000000
VERSION     DAJTRC010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9242)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9242)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9242
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.134"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            162..1697
                     /gene="gadC"
                     /locus_tag="OO357_003568"
     CDS             162..1697
                     /gene="gadC"
                     /locus_tag="OO357_003568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance gamma-aminobutyrate antiporter
                     GadC"
                     /protein_id="HCR5888382.1"
                     /translation="MATSVQTGKAKQLTLLGFFAITASMVMAVYEYPTFATSGFSLVF
                     FLLLGGILWFIPVGLCAAEMATVDGWEEGGVFAWVSNTLGPRWGFAAISFGYLQIAIG
                     FIPMLYFVLGALSYILKWPALNEDPITKTIAALIILWALALTQFGGTKYTARIAKVGF
                     FAGILLPAFILIALAAIYLHSGAPVAIEMDSKTFFPDFSKVGTLVVFVAFILSYMGVE
                     ASATHVNEMSNPGRDYPLAMLLLMVAAICLSSVGGLSIAMVIPGNEINLSAGVMQTFT
                     VLMSHVAPEIEWTVRVISALLLLGVLAEIASWIVGPSRGMYVTAQKNLLPAAFAKMNK
                     NGVPVTLVISQLVITSIALIILTNTGGGNNMSFLIALALTVVIYLCAYFMLFIGYIVL
                     VLKHPDLKRTFNIPGGKGVKLVVAIVGLLTSIMAFIVSFLPPDNIQGDSTDMYVELLV
                     VSFLVVLALPFILYAVHDRKGKANTGVTLEPINSQNAPKGHFFLHPRARSPHYIVMND
                     KKH"
     gene            1828..3147
                     /locus_tag="OO357_003569"
     CDS             1828..3147
                     /locus_tag="OO357_003569"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 10 protein"
                     /protein_id="HCR5888383.1"
                     /translation="MDICSRNKKLTIRRPAILVALALLLCSCKSTPPESMVTPPAGSK
                     PPATTQQSSQPMRGIWLATVSRLDWPPVSSVNISNPTSRARVQQQAMIDKLDHLQRLG
                     INTVFFQVKPDGTALWPSKILPWSDLMTGKIGENPGYDPLQFMLDEAHKRGMKVHAWF
                     NPYRVSVNTKPGTIRELNSTLSQQPASVYVQHRDWIRTSGDRFVLDPGIPEVQDWITS
                     IVAEVVSRYPVDGVQFDDYFYTESPGSRLNDNETYRKYGGAFASKADWRRNNTQQLIA
                     KVSHTIKSIKPGVEFGVSPAGVWRNRSHDPLGSDTRGAAAYDESYADTRRWVEQGLLD
                     YIAPQIYWPFSRSAARYDVLAKWWADVVKPTRTRLYIGIAFYKVGEPSKIEPDWMING
                     GVPELKKQLDLNDAVPEISGTILFREDYLNKPQTQQAVSYLQSRWGS"
     gene            3445..3486
                     /locus_tag="OO357_003570"
     CDS             3445..3486
                     /locus_tag="OO357_003570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888384.1"
                     /translation="MVQLVDLARCVSF"
     gene            3524..4669
                     /gene="dosC"
                     /locus_tag="OO357_003571"
     CDS             3524..4669
                     /gene="dosC"
                     /locus_tag="OO357_003571"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DosC"
                     /protein_id="HCR5888385.1"
                     /translation="MEMYFKRMKDEWTGLVEQADPLIRAKAAEIALAHAHYLSIEFYR
                     IVRIDPHAEEFLSNEQVERQLKSAMERWIINVLSAQVDDVERLIQIQHTVAEVHARIG
                     IPVEIVEMGFRVLKKILYPVIFSSDYSAAEKLQVYHFSINSIDIAMEVMTRAFTFSDS
                     SASKEDENYRIFSLLENAEEEKERQIASILSWEIDIIYKVLLDSDLGSSLPLSQADFG
                     LWFNHKGRHYFSGIAEVGHISRLIQDFDGIFNQTMRNTRILNNRSLRVKFLLQIRNTV
                     SQIITLLRELFEEVSRHEVGMDVLTKLLNRRFLPTIFKREIAHANRTGTPLSVLIIDV
                     DKFKEINDTWGHNTGDEILRKVSFLSQKRLVKSKILGAGSSRKLAVS"
     gene            4594..5211
                     /locus_tag="OO357_003572"
                     /pseudo
     CDS             4594..5211
                     /locus_tag="OO357_003572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416006.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
     gene            5469..5801
                     /gene="ddpX"
                     /locus_tag="OO357_003573"
                     /pseudo
     CDS             5469..5801
                     /gene="ddpX"
                     /locus_tag="OO357_003573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310119.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine dipeptidase"
     gene            5815..6711
                     /locus_tag="OO357_003574"
     CDS             5815..6711
                     /locus_tag="OO357_003574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D,D-dipeptide ABC transporter permease"
                     /protein_id="HCR5888386.1"
                     /translation="MMLSEETSAVRPQKQTRFNGAKLVWMLKGSPLTVTGAVIIVLMR
                     LMMIFSPWLATHDPNAIDLTARLLPPSAAHWFGTDEVGRDLFSRVLVGSQQSILAGLV
                     VVAIAGMIGSLLGCLSGVLGGRADAIIMRIMDIMLSIPSLVLTMALAAALGPSLFNAM
                     LAIAIVRIPFYVRLARGQALVVRQYTYVQAAKTFGASRWHLINWHILRNSLPPLIVQA
                     SLDIGSAILMAATLGFIGLGAQQPSAEWGAMVANGRNYVLDQWWYCAFPGAAILLTSV
                     GFNLFGDGIRDLLDPKAGGKQS"
     gene            6708..7682
                     /locus_tag="OO357_003575"
     CDS             6708..7682
                     /locus_tag="OO357_003575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HCR5888387.1"
                     /translation="MTQPVLDIQQLHLSFPGFNGDVHALNNVSLQINRGEIVGLVGES
                     GSGKSVTAMLIMRLLPTGSYCVHRGQISLLGEDVLNAREKQLRQWRGARVAMIFQEPM
                     TALNPTRRIGLQMMDVIRHHQPISRREARAKAIALLEEMQIPDAVEVMSRYPFELSGG
                     MRQRVMIAPAFSCEPQLIIAGEPTTALDVTVQLQVLRLLKHKARASGTAVLFISHDMA
                     VVSQLCDSVYVMYAGSVIESGGTADVIHHPRHPYTIGLLQCAPEHGVPRQPLPAIPGT
                     VPNLTHLPDGCAFRDRCYAAGAQCALTACGDNNQHCTCWYPQQEVISV"
     gene            7675..8601
                     /locus_tag="OO357_003576"
                     /pseudo
     CDS             7675..8601
                     /locus_tag="OO357_003576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416000.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
     gene            complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO357_003577"
     CDS             complement(8657..9088)
                     /gene="osmC"
                     /locus_tag="OO357_003577"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin OsmC"
                     /protein_id="HCR5888388.1"
                     /translation="MTIHKKGQAHWEGDIKRGKGTVSTESGVLNQQPYGFNTRFEGEK
                     GTNPEELIGAAHAACFSMALSLMLGEAGFTPTSIDTTADVSLDKVDAGFAITKIALKS
                     EVAVPGIDASTFDGIIQKAKAGCPVSQVLKAEITLDYQLKS"
BASE COUNT         2259 a         2257 c         2317 g         2409 t
ORIGIN      
        1 acctgataac gtttaacggt aacggtgtcc cgaaacgaac ccgtttcggg ataatttcca
       61 aagtctgttc actggcatta gcaacggaaa atattgttct gaatacgctt cagaacaaaa
      121 caggtgcggt tccgacagga ataccgtttt agggggataa tatggctaca tcagtacaga
      181 caggtaaagc taagcagctc acattactcg gattctttgc cataacggca tcgatggtaa
      241 tggctgttta tgaataccct accttcgcaa catcgggctt ttcattagtc ttcttcctgc
      301 tattaggcgg gattttatgg tttattcccg tgggactttg tgctgcggaa atggccaccg
      361 tcgacggctg ggaagaaggt ggtgtcttcg cctgggtatc aaatactctg ggtccgagat
      421 ggggatttgc agcgatctca tttggctatc tgcaaatcgc cattggtttt attccgatgc
      481 tctatttcgt gttaggggca ctctcctaca tcctgaaatg gccagcgctg aatgaagacc
      541 ccattaccaa aactattgca gcactcatca ttctttgggc gctggcatta acgcagtttg
      601 gtggcacgaa atacacggcg cgaattgcta aagttggctt cttcgccggt atcctgttac
      661 ctgcatttat tttgatcgca ttagcggcta tttacctgca ctccggtgcc cccgttgcta
      721 tcgaaatgga ttcgaagacc ttcttccctg acttctctaa agtgggcaca ctggttgtat
      781 ttgttgcctt cattttgagt tatatgggcg tagaagcttc cgcaactcac gtcaatgaaa
      841 tgagcaaccc cgggcgcgac tatccactgg ctatgttact gctgatggtg gcggcaatct
      901 gcttaagctc tgttggcggt ttgtctattg cgatggtcat tccgggtaat gaaatcaacc
      961 tctccgcagg ggtaatgcaa acctttaccg ttctgatgtc ccatgtggca ccggaaattg
     1021 agtggacggt tcgcgtgatc tccgcactgc tgttgctggg tgttctggcg gaaatcgcct
     1081 cctggattgt tggtccttct cgcgggatgt atgtcacagc gcagaaaaac ctgctgccag
     1141 cggcattcgc taaaatgaac aaaaatggcg taccggtaac gctggtcatt tcgcagctgg
     1201 tgattacttc tatcgcgttg atcatcctca ccaataccgg tggcggtaac aacatgtcct
     1261 tcctgatcgc actggcgctg acggtggtga tttatctgtg tgcttatttc atgctgttta
     1321 ttggctacat tgtgttggtt cttaaacatc ctgacttaaa acgcacattt aatatccctg
     1381 gtggtaaagg ggtgaaactg gtcgtggcaa ttgtcggtct gctgacttca attatggcgt
     1441 ttattgtttc cttcctgccg ccggataaca tccagggtga ttctaccgat atgtatgttg
     1501 aattactggt tgttagtttc ctggtggtac ttgccctgcc ctttattctc tatgctgttc
     1561 atgatcgtaa aggcaaagcg aataccggcg tcactctgga gccaatcaac agtcagaacg
     1621 caccaaaagg tcacttcttc ctgcacccgc gtgcacgttc accacactat attgtgatga
     1681 atgacaagaa acactaacca tgtctgaatg gttataagac aagggagcga taattcatcg
     1741 ctcccttttt cgtgcttgct gtcgtattga cttaaccggg ataagtacga gaatgagcgc
     1801 acatctgttt accggaaacc agcacatatg gatatctgct cccgaaacaa gaaattaacg
     1861 attagaagac cagcgatact ggttgcattg gcacttttac tgtgtagttg taaaagcacg
     1921 cctccagagt ccatggtgac accaccagca ggttcaaagc caccagccac gacgcaacaa
     1981 tcgtcacaac cgatgcgtgg catctggctg gccacggttt ctcgcctcga ctggccaccg
     2041 gtttcctcgg ttaacattag taatcccacc agccgagccc gtgtacaaca acaggcgatg
     2101 atcgacaaac tggatcatct ccaacgtctc ggcataaaca cggtcttttt ccaggtcaag
     2161 ccggacggta ccgccctgtg gccatcgaaa attttgccgt ggtccgatct tatgaccggt
     2221 aagattggtg aaaatccggg ttacgatccg ctgcaattca tgctcgacga agcccacaag
     2281 cgtgggatga aagtacacgc ctggtttaac ccctatcgcg tatcggttaa tacgaagccc
     2341 ggtactatca gggaactgaa tagcactctg tctcaacaac cggcgagcgt ctatgtgcaa
     2401 caccgtgact ggatcagaac gtctggcgat cgctttgtcc tcgacccggg catccctgag
     2461 gttcaggact ggatcacatc aatagtcgct gaagtggttt cccgctatcc ggtagatggc
     2521 gtgcagtttg acgactattt ctatactgaa tcaccgggtt cacggctaaa tgataacgaa
     2581 acgtaccgta aatacggagg cgcatttgcg tcaaaagcag actggcggcg caacaatact
     2641 cagcagttaa ttgcaaaggt atcacacacc attaaaagca ttaagccggg agtcgaattt
     2701 ggcgttagcc cggcaggcgt gtggcgtaac cgatcacacg atccgctcgg ttccgatacc
     2761 cgaggcgcgg cagcctatga cgaatcctac gcagacaccc gtcgatgggt ggaacaagga
     2821 ttgctggatt acattgctcc ccaaatttac tggccgttct cacggagtgc cgcgcgttat
     2881 gacgtgttgg caaaatggtg ggcggatgtc gttaaaccaa ccaggacccg cctgtatatc
     2941 ggtatcgcct tctataaagt gggtgaacct tcaaagatag agccagactg gatgattaac
     3001 ggcggcgtac cggaactgaa aaagcagctc gatcttaacg atgcggtacc agaaattagc
     3061 ggcaccatct tgttccgtga ggactatctg aataaaccgc agactcaaca agcggtcagc
     3121 tatctgcaaa gtcgttgggg cagttaaact accatgctta ctttgtaaca agccggtgct
     3181 ttacccaccg gcttgttaca ccttgtgaaa tatcccacta gtaatcatgc ttacataagt
     3241 caaattaacc acgcaattaa ccacacttag ttaaacagct ataaatccct aataaaaatg
     3301 tatgaatatg aaaaaccata cgatattcgc tgtcaccgaa aaataattac gatgggctat
     3361 actcttctga agaacagtag cgtagattga aactgtctta agtcgcttat ttcttttcag
     3421 tatatcttca tatttcagga gaatatggtg cagttagtcg atctcgcacg ttgcgtttcg
     3481 ttttgattcc gtctgatttc ccagccttat aagggtgtga attatggaga tgtattttaa
     3541 aagaatgaaa gatgagtgga ccggacttgt cgaacaggca gatcctctca ttcgtgctaa
     3601 agccgcagaa attgcccttg cgcatgctca ttatctgagt atcgagtttt atcgaattgt
     3661 ccgcatcgac ccgcatgccg aagaattctt gagtaatgaa caagttgagc ggcagttgaa
     3721 aagtgcgatg gaacgctgga ttattaacgt gctttctgcc caggttgacg atgtcgaaag
     3781 gctaatacaa atccagcata ccgtcgcgga agtgcatgcc cgcataggaa ttccggtaga
     3841 aattgtcgag atggggtttc gggtgctgaa aaaaatcctc tatccggtaa tcttctcttc
     3901 ggattattcc gccgcagaaa aacttcaggt ctaccatttc tcgattaaca gtattgatat
     3961 cgcgatggaa gtgatgaccc gcgcgtttac ctttagtgac agtagtgcct caaaggaaga
     4021 tgaaaactat cgtatcttct cgttactgga aaacgccgaa gaagaaaaag aacggcaaat
     4081 agcctcaata ctttcatggg aaatagatat tatctataaa gtcctgctgg attctgattt
     4141 aggcagcagt ttgcctttaa gccaggctga ttttggcctg tggtttaacc ataaaggtcg
     4201 acattatttt agcggcattg ccgaagtggg ccatatctcc cgtctgattc aggatttcga
     4261 cggtattttc aatcaaacca tgcgtaacac caggattttg aataacagaa gtttgcgggt
     4321 gaaatttcta ttacagataa gaaataccgt atcgcagatt attaccttgc tgcgtgaatt
     4381 gtttgaagaa gtatcgcgcc acgaagtcgg tatggatgta ctgacgaaat tacttaaccg
     4441 ccgtttcctg ccgactatct tcaaacgcga aattgcccat gccaaccgga ccggtacacc
     4501 gctgtcagtg ctgattattg acgttgataa attcaaagag atcaacgata cgtggggcca
     4561 taacactggc gatgaaattc tgcgtaaagt ctcattcctc tcgcagaaga gattggtgaa
     4621 atcgaaaata ttgggcgctg ggtcatcgcg gaagcttgcc gtcagttagc agaatggcgt
     4681 agccagaata ttcatatccc ggcgttatcc gtgaacttgt cggcgctgca ctttcgcagt
     4741 aatcagctgc ctaatcaggt gtctgatgca atgcaagcct ggggtattga cggccaccag
     4801 ctgacggtgg aaatcacgga aagcatgatg atggaacacg ataccgaaat ctttaagcgc
     4861 attcagatcc tgcgtgatat gggcgtgggt ttatcggtag atgattttgg cacgggcttt
     4921 tccggattat cccgcttagt cagtcttccg gtaacggaaa tcaaaattga caaaagcttt
     4981 gtcgatcgtt gtctgacgga aaaacgcatc ctggccttac ttgaagccat taccagcatt
     5041 gggcaaagcc tcaatttaac cgtcgtggcg gaaggggtcg aaaccaaaga gcaatttgag
     5101 atgctacgta agatccactg tcgcgttatt cagggatatt tcttttcccg cccccttccc
     5161 gccgaagaaa ttccaggctg gatgagcagc gtgttaccgc tgaaaatctg acaaattcct
     5221 ctcgcccgca ctcgcgggtt ttcctttaag gtgacactgt cacttaaata ctgtgatttc
     5281 agctacgatt ccgggagatt ccttccttaa ctttccctct tgcaccaaaa ctgcactacg
     5341 ctgcacaaat gttgatcaaa agttaaaaaa tataacttta agccagcatt ctggcgctta
     5401 tcccggcatg gcatgagatc tgcataagcg gaaagcgcag caatttttgt cttatacagg
     5461 aaccgtttat gtcggatacc accgaactgg ttgatttagc cgtgatcttc cctgatctgg
     5521 agatcgaatt gaaatatgcc tgcgttgata acatcacagg taaagctatt tatcagcaag
     5581 cgcgttgtct gctacataaa gatgcgatta ccgcgctggc gaaaagtatc agtatcgccc
     5641 agctgtcagg cttacaactg gtgatttacg atgcgtatcg cccacaacaa gcacaggcga
     5701 tgttgtggca agcctgccca gacccgcaat atgttgttga tgtgacggtc ggttctaatc
     5761 acagccgtgg cacggcgctc gacctgacgc ttcgtgatga cgtggaggtg gtgaatgatg
     5821 ctaagcgagg aaacgtccgc cgtacgccca caaaaacaaa cgcgatttaa cggtgcaaaa
     5881 ctggtatgga tgctgaaagg cagtccgctc accgtgaccg gcgcagtcat cattgtatta
     5941 atgcgactga tgatgatttt ttcaccgtgg ctggcgacgc atgatcccaa cgccattgat
     6001 ttaaccgccc gccttttgcc gccttctgcg gcgcactggt ttggcaccga tgaagtggga
     6061 cgcgatctgt ttagccgcgt actggtcggc agtcagcaat caattctcgc cggattagtg
     6121 gtggtcgcca ttgcgggtat gattggttcg ctactcggat gtctatccgg tgtgcttggc
     6181 ggacgcgcag acgccatcat catgcgcatc atggacatta tgctgtcgat tccttcgttg
     6241 gtactgacaa tggcactggc tgccgctctc gggccgagtt tatttaacgc catgctggcg
     6301 attgctattg tgcgaattcc cttttatgtg cgcttggcgc gggggcaagc attagttgta
     6361 cgccagtata cctatgttca ggcggcgaaa acctttggcg cgtctcgttg gcatctgatc
     6421 aactggcata ttttacgtaa ctccctaccg ccgctgatcg tgcaggcatc gctggatatc
     6481 ggtagcgcga ttttaatggc cgccacgttg ggatttattg gcctgggtgc tcaacaaccg
     6541 agtgctgaat ggggcgcgat ggtagcgaat ggtcgcaact atgtactcga tcagtggtgg
     6601 tattgcgcat ttccgggggc agcgattttg cttacctccg tcgggtttaa tctctttggt
     6661 gatggtattc gcgatctgct tgacccgaaa gcaggaggaa agcagtcatg acccaacccg
     6721 ttctggacat tcaacagctg catttgagtt tccccggttt taacggtgat gttcacgcgc
     6781 tcaacaatgt gtccttgcag attaaccgcg gcgaaattgt cggtctggtg ggagaatccg
     6841 gctcaggtaa atcagtcacc gcaatgctga ttatgcgtct gctaccgacg ggcagttatt
     6901 gcgtacatcg ggggcagatt tcactgctgg gagaagatgt tcttaacgct cgggaaaaac
     6961 agcttcgtca gtggcgcggc gcacgagtgg cgatgatctt tcaggaaccg atgaccgccc
     7021 ttaatccgac acgtcgaata ggtcttcaga tgatggacgt aatccgccat catcaaccaa
     7081 taagtcgtcg ggaagccaga gctaaagcga ttgcactgct ggaagagatg caaatcccgg
     7141 atgccgtgga agttatgtcg cgctatccgt ttgagctttc aggtggtatg cgccagcggg
     7201 taatgattgc gccggcattc tcctgcgagc cgcaattgat tattgccggc gaaccgacga
     7261 cggcgctgga cgtcacagta cagttgcagg tactgcgttt gcttaaacat aaagcacgcg
     7321 ccagtggaac tgcggtactg ttcatcagcc atgatatggc cgtggtgtcg caactgtgcg
     7381 atagcgttta cgtgatgtat gccggaagcg taattgaaag cggcgggacg gcagacgtta
     7441 tccatcatcc ccggcatccg tataccattg gtttgctgca atgcgcacca gaacatggag
     7501 taccacgcca gccattaccc gccattccag ggacggtacc aaacctcacc catttgcctg
     7561 acggctgcgc ttttcgcgat cgttgctatg cggcaggtgc acagtgtgcg ctgacagcgt
     7621 gtggtgacaa caaccagcac tgcacctgtt ggtatccgca gcaggaggtc ataagtgtct
     7681 gacacgttat taacgttacg cgacgtccat atcaattttc cggcccgtaa aaactggctt
     7741 ggcaaatcta cggaacatgt tcatgccatt aatggtattg atttacagat ccgccgtggt
     7801 gaaaccttag ggatcgtcgg cgagtcaggc tgcggcaaaa gcaccctcgc acagctttta
     7861 atgggcatgc tgcaaccgag ccacgggcag tacatccgtt caggctcaca acgcattatg
     7921 cagatggtgt ttcaggaccc gctctcttcg cttaatccgc gcttaccggt gtggcgcatc
     7981 atcacagaat cgctctggat agctaagcgt agtagtgaac aacagcggcg agcgttggca
     8041 gaggagttgg ctgtgcaggt gggtattcgt ccggagtatc tcgaccgcct gcctcatgcg
     8101 ttctccggcg ggcagcggca acgcatcgcc attgccagag cactctcttc gcagcctgac
     8161 gtgattgtgc ttgatgagcc aacctcggcg ctggatatct ccgtgcaggc gcagatcctc
     8221 aatttgctgg taacgctaca ggaaaatcac gggctgactt atgtgctgat ttcacacaat
     8281 gtctcggtga tacgtcatat gagcgatcgg gtggcggtga tgtatctcgg gcagattgta
     8341 gaactgggcg acgcgcaaca ggtgttgact gcttcggcac atccatacac ccgattattg
     8401 ctggattccc tccccgccat tgataaaccg ctggaggaag aatgagcatt acgtaaaacg
     8461 gatctgccgg gaaaccgcac gttgccgcaa ggctgttttt tctacgaacg ttgccctctg
     8521 gcaacccacg gatgtgaagt ccggcaatca ttagcaataa gggaggacgg acgtgacctc
     8581 cggtgctggc gggcgctgta gagatgctaa caacgcatca ggcattcacg cgcctgacgc
     8641 gtcatccggc aatgctttac gatttcaact ggtaatccag cgtaatttcc gctttcagta
     8701 cctgagagac cgggcatcct gcttttgctt tctggatgat gccgtcaaag gtagaggcat
     8761 caatacccgg caccgcaact tcactcttca gtgcgatttt cgtaatcgca aaaccggcat
     8821 ccactttatc cagcgacaca tcggcggtgg tatcaattga tgttggcgtg aatcccgctt
     8881 cccccagcat taatgaaagc gccattgaga aacatgcggc atgcgctgcg ccaatcagtt
     8941 cttcagggtt ggttcctttt tcgccttcaa aacgcgtgtt aaatccatac ggctgttggt
     9001 tcagcacgcc gctctcggtg gatactgttc ccttcccgcg tttgatatcg ccttcccagt
     9061 gtgcctgacc tttcttatgg attgtcattg ttgctctcct gtgggcttaa aaaggaaatt
     9121 atagactacg tgagaaacag cacgaggata agcaggatat tccgaataaa atacggcgaa
     9181 aatattactt ctgcacttaa attaaattcc cacaatagat aaccgggaat atatatctca
     9241 ag
//
LOCUS       DAJTRC010000135         9182 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.135, whole genome shotgun sequence.
ACCESSION   DAJTRC010000135 DAJTRC010000000
VERSION     DAJTRC010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9182)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9182)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9182
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.135"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..894)
                     /locus_tag="OO357_003578"
     CDS             complement(<1..894)
                     /locus_tag="OO357_003578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HCR5888389.1"
                     /translation="MNRRRKLLIPLLFCGAMLTACDDKSAENAAAMTPEVGVVTLSPG
                     SVNVLSELPGRTVPYEVAEIRPQVGGIIIKRNFIEGDKVNQGDSLYQIDPAPLQAELN
                     SAKGSLAKALSTASNARITFNRQASLLKTNYVSRQDYDTARTQLNEAEANVTVAKAAV
                     EQATINLQYANVTSPITGVSGKSSVTVGALVTANQADSLVTVQRLDPIYVDLTQSVQD
                     FLRMKEEVASGQIKQVQGSTPVQLNLENGKRYSQTGTLKFSDPTVDETTGSVTLRAIF
                     PNPNGDLLPGMYVTALVDEGSR"
     gene            954..1232
                     /locus_tag="OO357_003579"
     CDS             954..1232
                     /locus_tag="OO357_003579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888390.1"
                     /translation="MRDLQTSGIVGLSASKVGYRHSDRHGEVNDKKNVAVLPTVPASI
                     RATKGSTGGYTYQGNQVVLASPLVITGGNEISICIPRHASHQFQLLMS"
     gene            complement(1233..1760)
                     /gene="gadE"
                     /locus_tag="OO357_003580"
     CDS             complement(1233..1760)
                     /gene="gadE"
                     /locus_tag="OO357_003580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadE"
                     /protein_id="HCR5888391.1"
                     /translation="MIFLMTKDSFLLQGFWQLKDNHEMIKINSLSEIKKVGNKPFKVI
                     IDTYHNHILDEEAIKFLEKLDAERIIVLAPYHISKLKAKAPIYFVSRKESIKNLLEIT
                     YGKHLPHKNSQLCFSHNQFKIMQLILKNKNESNITSTLNISQQTLKIQKFNIMYKLKL
                     RRMSDIVTLGITSYF"
     gene            complement(2559..3131)
                     /gene="hdeD"
                     /locus_tag="OO357_003581"
     CDS             complement(2559..3131)
                     /gene="hdeD"
                     /locus_tag="OO357_003581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-resistance protein HdeD"
                     /protein_id="HCR5888392.1"
                     /translation="MLYIDKATILKFDLEMLKKHRRAIQFIAVLLFIVGLLCISFPFV
                     SGDILSTVVGALLICSGIALIVGLFSNRSHNFWPVLSGFLVAVAYLLIGYFFIRAPEL
                     GIFAIAAFIAGLFCVAGVIRLMSWYRQRSMKGSWLQLVIGVLDIVIAWIFLGATPMVS
                     VTLVSTLVGIELIFSAASLFSFASLFVKQQ"
     gene            3386..3718
                     /gene="hdeA"
                     /locus_tag="OO357_003582"
     CDS             3386..3718
                     /gene="hdeA"
                     /locus_tag="OO357_003582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeA"
                     /protein_id="HCR5888393.1"
                     /translation="MKKVLGVILGGLLLLPVVSNAADAQKAADNKKPVNSWTCEDFLA
                     VDESFQPTAVGFAEALNNKDKPEDAVLDVQGIATVTPAIVQACTQDKQANFKDKVKGE
                     WDKIKKDM"
     gene            3834..4160
                     /gene="hdeB"
                     /locus_tag="OO357_003583"
     CDS             3834..4160
                     /gene="hdeB"
                     /locus_tag="OO357_003583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid-activated periplasmic chaperone HdeB"
                     /protein_id="HCR5888394.1"
                     /translation="MNISSLRKAFIFMGAVAALSLVNAQSALAANESAKDMTCQEFID
                     LNPKAMTPVAWWMLHEETVYKGGDTVTLNETDLTQIPKVIEYCKKNPQKNLYTFKNQA
                     SNDLPN"
     gene            4224..4870
                     /locus_tag="OO357_003584"
                     /pseudo
     CDS             4224..4870
                     /locus_tag="OO357_003584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417965.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
     gene            complement(4912..5442)
                     /locus_tag="OO357_003585"
     CDS             complement(4912..5442)
                     /locus_tag="OO357_003585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709290.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5888395.1"
                     /translation="MFLIITRDTMFFTAMKNILSKGNVVHIQNEEEIDVMLHQNAFVI
                     IDTLMNNVFHSNFLTQIERLKPVHVIVFSPFNIKRCLGKVPVTFVPRTITIIDFVALI
                     NGSYCSVPEANVSLSRKQHQVLSCIANQMTTEDILEKLKISLKTFYCHKHNIMMILNL
                     KRINELVRHQHIDYLV"
     gene            complement(5598..6164)
                     /gene="slp"
                     /locus_tag="OO357_003586"
     CDS             complement(5598..6164)
                     /gene="slp"
                     /locus_tag="OO357_003586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane lipoprotein Slp"
                     /protein_id="HCR5888396.1"
                     /translation="MNMTKGALILSLSFLLAACSSIPQNIKGNNQPDIQKSFVAVHNQ
                     PGLYVGQQARFGGKVINVINGKTDTLLEIAVLPLDSYAKPDIEANYQGRLLARQSGFL
                     DPVNYRNHFVTILGTIQGEQPGFINKVPYNFLEVNMQGIQVWHLREVVNTTYNLWDYG
                     YGAFWPEPGWGAPYYTNAVSQVTPELVK"
     gene            complement(6411..7634)
                     /locus_tag="OO357_003587"
     CDS             complement(6411..7634)
                     /locus_tag="OO357_003587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888397.1"
                     /translation="MSIDFTPGIINTYHGDIYNCTTNTDNVKTPDTPKWPCDNWEEQQ
                     PINSTFSGEGYNPEQFDLAQQQLQQINACHTNTTYTNADYSKVVVQLVSLINNIETIS
                     STPLTQQTQSILNQIHNIRYEKDKDSVCQIIVVVNPEPNNPIITKILVEEGIPERFSV
                     QTVSSDNKNFAGQRADLPTDIRDIQSLYLKMAKLYIEHSKNENRMEALAGCDFIDFNM
                     TGQDMSKLVLTLSKFYFEDLLNINFTDANLFNTIFSHKENPIPKLHKYEQHLDKQING
                     LFSTLLTINDNSLRAKAEIASRIIDFLEAKVVNLSFDDILKYKQEFKKICYKQLQEFT
                     TPSLYNKIQKWATMSKNEFIEFHYETLQPEKISYPFYLKRDLPNEKDINYGVEIEIPS
                     GKRIRLSNHYQNIIP"
     gene            complement(8215..>9182)
                     /locus_tag="OO357_003588"
     CDS             complement(8215..>9182)
                     /locus_tag="OO357_003588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000100290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="permease"
                     /protein_id="HCR5888398.1"
                     /translation="EMFVFLAVELSLLFIVISAGVSLIRQKVPDHKIQQMMGARKGRG
                     YLLAALLGAVTPFCSCSTIPMLRGLLSAKAGFGPTLTFLFVSPLLNPIIVGLMWMTFG
                     WKVTLLYAIIAAGVSVLASIILDSLGFERHIIASKSSSANCCAPAKTSPGTTYTPIKV
                     SCCSPAAKAIENPVVTCCSTKAVVSINPIKLATKDALQQFKDVLPYLLLGVLIGSFIY
                     GFIPSAWIAAHAGSDNPFAIPLSAVVGIPLYIRAEAVIPLASVLMTKGMGLGALMALI
                     IGSAGASLTEVILLKSMFRMPMIAAFLTVILGMAILMGYLTQLLF"
BASE COUNT         2679 a         1824 c         1922 g         2757 t
ORIGIN      
        1 gcggctacct tcatccacta atgccgtgac atacatgcca ggcagcaagt caccatttgg
       61 gttggggaaa atcgcccgta acgtcacgga gcccgtggtt tcatcaaccg tcgggtcgga
      121 gaatttcagc gtgccggtct ggctgtaacg tttaccattt tccagattga gctgtactgg
      181 cgtactgccc tgaacctgtt tgatttgccc actggcgacc tcttctttca tgcgtaagaa
      241 atcttgcacc gactgcgtga gatcgacata aatcgggtcc agacgttgta cggtaaccag
      301 cgaatctgcc tgattagcgg taacgagtgc gccgacggtc accgacgatt tcccgctgac
      361 gcccgtaatc ggcgaggtga cattcgcgta ttgcagattg atcgtcgcct gttcaacagc
      421 cgctttggcg acggtgacat tggcttctgc ttcattcagc tgggtgcgcg cggtgtcgta
      481 atcctgacgg ctaacgtagt tggtcttcag caacgatgcc tggcggttaa aggtgatgcg
      541 ggcattgctg gcggtagaga gcgctttcgc cagcgagcct ttggcggagt ttagctcggc
      601 ctgtaaaggt gcaggatcaa tctgatacag cgaatcgccc tggttcactt tatcgccttc
      661 gataaagttg cgtttaatga taataccgcc cacctgggga cgtatctcgg caacttcata
      721 aggaacggtt ctaccgggca attcgctcaa cacattgacc gaaccagggg agagtgtgac
      781 gacaccgacc tcaggcgtca tggcggcggc gttttccgcc gatttgtcat cgcaggcggt
      841 gagcatcgcg ccgcagaata acaacggtat taacagcttt cttcttctgt tcattttagt
      901 ccctgaaaat tcttgagaaa taacggttct ttaaattgcg cgcgaggaga gatatgaggg
      961 atttacagac cagcggcata gtggggttgt cagccagtaa ggtgggatac aggcacagtg
     1021 atcgacatgg tgaggtcaac gacaaaaaaa atgttgccgt tctgccaaca gttcctgcca
     1081 gcattcgggc aacgaagggt tctactggtg gatacacata ccaggggaat caagtcgttt
     1141 tagcctctcc tttagtaatc accgggggta atgaaatcag catctgcatc cctcgtcatg
     1201 ccagccatca atttcagttg cttatgtcct gactaaaaat aagatgtgat acccagggtg
     1261 acgatgtcgc tcatacgtct tagtttcagc ttgtacataa tgttgaattt ctgaatcttt
     1321 aatgtttgtt gcgaaatatt gagcgtcgac gtgatattgc tttcattttt atttttcaga
     1381 atcagttgca taattttgaa ctgattatgt gaaaaacata attgtgaatt cttatggggc
     1441 aagtgtttac cataagtaat ctcaagaaga tttttgatac tttctttgcg gctaacaaaa
     1501 taaataggcg ctttagcttt tagtttactg atgtgataag gtgccaaaac aataattctc
     1561 tcggcatcta atttctccag aaatttaatc gcttcttcat caaggatatg attgtgatag
     1621 gtatcaatga taaccttgaa gggtttattg cctacttttt tgatctctga cagggaattg
     1681 atttttatca tttcgtgatt atctttcaac tgccaaaagc cctgtaaaag aaaagaatct
     1741 ttcgtcatga gaaaaatcat aacttgctcc ttagccgtta tcgtttcgaa catgtcatcc
     1801 ttttcattat ttacatcctt gtccgaatcg ttgttcaata tagtaaacgc ctataagaac
     1861 tttgcccttc gtgaactaat acgtctatta acactaatca atacttcaga tttatccatc
     1921 acgcactcca tatattgatc ctaagtaaga ttcctggttg ttatcagctt gtaaatagta
     1981 aacaaaaagt taacgtttgc ggattactgc ccaagaataa gttatcaata accagtttgt
     2041 gatctctgaa gaatattact aaagttaaaa tgtaatccga tttaaatatc gagtctcctt
     2101 gtttcgactt aagctggcaa ttggattgcc agctttcttt taacgcattg aaattacctg
     2161 agtaaataac gcattcatgt cttcaggttt ttatatagcg tctggagaca cggatattta
     2221 tgcaatgtaa tcaacgccag ttgcaaaaaa atgaacccgg attacttatt taaccgccca
     2281 tagcatcaac gcttacggaa ctctttgttc actttcccac agcactttta aaacattaaa
     2341 cctgactacg gaaaatatca gccatgataa tgtttgaaat ggctaatttg ccatggagtg
     2401 aaaaaaatta gatgaaattc agtaggttga aataatcact agcaggtaat tatttcaatg
     2461 atagtgcgca attgatctac aacactgcgt agcggagaga gtattaatcg gatcatagtc
     2521 acatcaagtg actatgatcc gggtgacaac cggggtaatt attgctgctt aacgaacaaa
     2581 ctggcgaagc tgaacaggct ggcggcgcta aatatcagtt caattcccac cagtgtggaa
     2641 accagcgtta cagacaccat cggcgttgca ccaaggaata tccaggcaat gacgatatcc
     2701 agcacaccaa taacgagctg tagccagctg cctttcattg aacgctgacg ataccaactc
     2761 atcaggcgaa taacccctgc aacacagaac aaaccggcaa taaatgccgc aatggcaaaa
     2821 atgcccagct ccggtgcacg gatgaagaaa tagccgatca ataaataggc gactgcgacg
     2881 aggaaaccgg ataataccgg ccagaaatta tgactgcggt tgctgaataa cccgacaata
     2941 agcgcaatac ccgagcagat taataatgca cccactactg tgcttaaaat atcgccagag
     3001 acgaacggga aactgataca cagcaacccg acgataaaca gcagcacggc aataaactgg
     3061 attgctctgc gatgcttttt cagcatctcc agatcaaact tcaaaattgt tgccttatct
     3121 atatataaca tagaaccacc ctataaaatt aagaagaaaa tcccctgcta tcaatctatg
     3181 ccaaaaacgc gtctaagaat gcagtcgatt taataaaaat ttcctaattg cagtatctga
     3241 tgcatctgta actcattgta ttgaaataaa aatatctgat tttgatattt tccatcaaca
     3301 tgacatatac agaaaaccag gttataacct cagtgtcgaa attgattcgt gacggctctt
     3361 tcactttata gttgaggata ttacgatgaa aaaagtatta ggcgttattc ttggtggtct
     3421 gcttcttctg ccagttgtga gcaatgcagc ggatgcgcaa aaagcagctg ataacaaaaa
     3481 accggtcaac tcatggacct gtgaagattt cctggctgtg gacgaatcct tccagccaac
     3541 tgcagttggt tttgctgaag cgctgaacaa caaagataaa ccagaagatg cggttttaga
     3601 tgttcagggt attgcaaccg taaccccagc tatcgttcag gcttgtactc aggataaaca
     3661 agccaacttt aaagataaag ttaaaggcga atgggacaaa attaagaaag atatgtaatt
     3721 ccgggaatgc gttacatcgt acttccttgc atattgaaca ggccggaata tcttctttaa
     3781 aagcagctat tcctcctgtt catatataat ctctatattg aatgggttac aaaatgaata
     3841 tttcatctct ccgtaaagcg tttattttta tgggcgctgt agcggctttg tcactggtga
     3901 acgcacaatc tgcgttggca gccaatgaat ctgctaaaga tatgacctgc caggaattta
     3961 ttgatctgaa tccaaaagca atgaccccgg ttgcatggtg gatgctgcat gaagaaacag
     4021 tatataaagg tggcgatacc gttactttaa atgaaaccga tctcactcaa attcctaaag
     4081 tgatcgaata ctgtaagaaa aacccgcaga aaaatttgta taccttcaaa aatcaagcat
     4141 ctaatgactt gccgaattaa tgaggtacaa gtaaaaagga gtagcaagtt gagccatctt
     4201 gctgctcctt tttgcatttt tatatgacag cagaatttat tatacgtctt atactcgcgg
     4261 caattgcctg tggcgctatt ggcatggaaa ggcaaatgcg cggcaaagga gcagggttac
     4321 gcacacatgt attaattggc atgggaagcg ccctgtttat gattgtttcg aaatatggtt
     4381 ttgctgacgt gctgtcttta gatcacgtcg gactcgaccc cagccgtatt gctgctcagg
     4441 tggtgacggg cgtcgggttt atcggtgcag gtaacattct cgttcgtaac caaaatattg
     4501 taggtctgac gacggcagcg gatatctggg tgaccgccgc ataggtatgg ttattggcag
     4561 cggtatgtac gaactgggta tttatggttc agtgatgacc ttgttggtgc tggaagtctt
     4621 ccatcaatta accttccgcc tgatgaacaa aaattaccat ttacagctga cattagtgaa
     4681 cggtaatacg gtatcaatgc tcgactggtt taagcagcaa aaaataaaaa ctgatttagt
     4741 ttcgttgcag gaaaatgaag atcatgaagt ggtcgccatt gatatccagc ttcatgccac
     4801 aacgttgata gaagacctgt tgcgattatt aaaaggtatg gcaggggtga aaggcgtttc
     4861 aattagctaa tctgtgtggc agcgtagcca gactcaccgt aagcctgaaa ttcacaccag
     4921 ataatcaata tgctgatggc gtaccagctc attgatccgc ttaagattga ggatcatcat
     4981 gatattgtgt ttatggcagt agaacgtttt tagcgatatt ttcagtttct ccagaatatc
     5041 ttcagttgtc atttgattcg caatgcagct cagaacctga tgttgcttgc gcgaaagtga
     5101 cacattcgct tcaggcacag agcagtaact gccattgatg agtgcgacaa aatcaatgat
     5161 agtgatagtc cgcggaacaa aggtcaccgg tactttcccc agacagcgtt taatattaaa
     5221 gggggagaaa acaatgacat ggacaggttt taatcgttca atttgagtga gaaaattaga
     5281 atgaaataca ttattcatta atgtatcaat aatgacgaag gcattctgat gcaacattac
     5341 gtcgatctct tcttcgttct gtatatgaac gacattacct ttactcagaa tgtttttcat
     5401 cgcggtgaag aacatcgtat ccctggtaat tataagaaac ataaccgact cctggtgcgg
     5461 acattaactt tataatcagt ttgccaaagg taattaacag gacagggctt ataataataa
     5521 tagttagctt actgtatatt aattttatgt aatacatgat gcagatgctg agaaagcatc
     5581 tgcatctttc ggtggtgtta tttgaccagc tcaggtgtta cctgactcac cgcattggtg
     5641 tagtaaggcg caccccagcc cggttccggc cagaatgcac catagccgta atcccacagg
     5701 ttataggtgg tattaaccac ttctctcaaa tgccacacct ggatgccctg catattcact
     5761 tccaggaagt tatacgggac tttattgata aagcctggtt gttcaccctg aatggtaccg
     5821 aggatggtaa caaagtgatt acgatagttc actggatcaa ggaagccgct ttgtctggcg
     5881 agcagtcggc cctgatagtt ggcttcaata tcaggcttcg catagctatc caacggtaat
     5941 acagcgattt ctaacaacgt atccgttttg ccattgataa cgttgataac cttcccacca
     6001 aagcgcgctt gttgaccaac atataacccc ggctggttat gaacagcaac aaaacttttt
     6061 tgaatatcag gttggttatt gcctttgata ttttgcggaa ttgaactaca tgcggcaagc
     6121 aaaaatgaaa ggctgaggat gagtgcacct tttgtcatgt tcatgttact atccttatca
     6181 acaaatatta aaaccataat agatatttct ttatagtttt catctgattc tgaggtgata
     6241 tgcctgatag taacgtaaac ctgtctgatg tttatatccc ttctgtttag ttcaattccg
     6301 ttattttggg gcgtggtatg agtatccatc gcccaataat ataacccttt gttttaaaat
     6361 tacataaaat ctatttttat tcaatgatta taaagtgcta ataatcaata ttatggtatt
     6421 atattctgat aatgatttga taagcgaatt cttttgccag atggtatttc tatttccacc
     6481 ccataattta tatctttttc attaggcaga tctctcttta aataaaaggg gtatgaaatc
     6541 ttttcgggtt gaagtgtctc gtaatgaaat tctatgaatt catttttaga cattgttgcc
     6601 catttttgaa ttttattgta tagagagggt gttgtaaact cttgaagttg tttataacat
     6661 atttttttga actcttgctt atattttaat atgtcatcaa atgaaagatt cactaccttt
     6721 gcctctaaga aatcaattat tctcgaagca atttccgctt tcgcgcgtaa agagttgtca
     6781 ttaatagtta acaatgtaga aaacaaacca ttgatttgtt tgtcaagatg ttgttcatat
     6841 ttatgtagtt tggggatggg attttccttg tgtgaaaaaa tagtgttaaa taaatttgcg
     6901 tcagtgaaat ttatattaag tagatcttca aaataaaatt ttgataaggt taggactagc
     6961 ttggacatat cttgccctgt catgttgaaa tcaataaagt cgcatccagc aagggcttcc
     7021 atcctatttt catttttact gtgctcaata tacagcttcg ccatttttaa gtacaagctt
     7081 tgaatgtctc ttatatcagt tggtaagtca gctcgttgcc cagcaaaatt tttattatcg
     7141 gaagaaacgg tttggacact gaatctttcg ggtattcctt cttctacgag aattttagtt
     7201 atgattggat tgttgggttc tggattaacg acaacaatga tttgacatac agaatcctta
     7261 tccttttcgt atctaatgtt atgaatctga ttaagtattg attgtgtctg ttgcgtaagt
     7321 ggtgtcgaac ttatcgtttc aatgttatta ataagactta caagttgaac cacaactttt
     7381 gagtagtctg catttgtata tgtggtgttc gtgtggcatg cattaatttg ttggagttgt
     7441 tgttgcgcta aatcaaattg ttcagggttg tatccttctc cagagaatgt ggaattaatg
     7501 ggttgttgtt cttcccaatt atcacaaggc cacttcggtg tgtctggcgt tttaacatta
     7561 tcggtatttg ttgtgcagtt ataaatatcg ccgtgatatg tatttattat acctggggta
     7621 aagtcaattg acataacatt ccttattcat catctgtttt atgctaatgt ttttaaattg
     7681 gataattaga acaggaatgg attttcttct acacacaata aatataatgc gttatatctt
     7741 cctgatataa ttacctttaa attccctttc atttccaccc ggtaatattt gtgtataaaa
     7801 aaagggcagt gatagtatcg taaaggaaaa cgttaacaac tatcaaaaaa cgctcatttt
     7861 aaaaagttat tatgttctcc gatagtgtgt gaagtaatat ttttgtgatg ttttagatgt
     7921 gtaataatga accaggttaa agtgtattta aaaatgtgtt gtattagcga ctgaaatctg
     7981 ccttttaaaa aggaggaggg tactgagaca aagagtcatt gcttctccga taaaatgtca
     8041 gagaagcatg attacgtggt tattgtctgt tgatgacttg ttgaccgagc cgtcatcatt
     8101 ctcaatgcat tagttaaatc atttgtgatt tttaaggatt atcctgtcta tgattttcta
     8161 cttagaagga tatggcacta actatgcatc ttatagttta ctgctggcat ctatttaaaa
     8221 tagcaattga gtcaaatagc ccatcaagat agccatacct aatataaccg tcaggaatgc
     8281 agctatcatc ggcattctga acattgattt aagtaatatc acttccgtca ggcttgcgcc
     8341 ggcactgccg atgattaatg ccattaatgc tcccagaccc attccttttg tcatcagaac
     8401 agatgccaga gggataaccg cctcagcccg gatatacagc ggaataccga caacggcgct
     8461 taatgggatg gcgaagggat tatctgatcc tgcatgagcg gcaatccacg ctgagggaat
     8521 aaagccataa ataaaagatc ctattaatac tcctaacaaa agatatggca gtacatcttt
     8581 aaactgttgc aacgcatctt tggtggctag ttttatggga ttaatactta ctacagcttt
     8641 ggtactgcaa caggtaacta cggggttctc aatggcttta gccgctggac tacaacaact
     8701 cacttttatg ggcgtatatg tcgttcccgg cgaagttttt gctggagcac aacaatttgc
     8761 tgatgagctt ttactggcaa tgatatgacg ttcaaacccc agagaatcca ggataatact
     8821 ggcaagaacg gagacgccgg ctgcgataat cgcgtacaac aaggtgactt tccaaccaaa
     8881 ggtcatccac attagtccga cgataatggg attaagtaat ggtgaaacaa ataaaaaagt
     8941 gagagtcgga ccaaacccgg ctttcgctga caacaatcca cgtaacatgg gtattgtcga
     9001 gcaactacag aacggggtaa cggctcccaa cagagcagcc aggagatagc ctctcccttt
     9061 tctggccccc atcatctgct ggattttatg gtctggcacc ttttgtctta tcaggctgac
     9121 accggcacta atcactataa acagcaacga aagctcgacg gcgagaaaca caaacatctc
     9181 tg
//
LOCUS       DAJTRC010000136         9029 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.136, whole genome shotgun sequence.
ACCESSION   DAJTRC010000136 DAJTRC010000000
VERSION     DAJTRC010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9029)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9029)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9029
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.136"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OO357_003589"
     CDS             complement(97..1473)
                     /gene="trkA"
                     /locus_tag="OO357_003589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004125888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk system potassium transporter TrkA"
                     /protein_id="HCR5888399.1"
                     /translation="MKIIILGAGQVGGTLAENLVGENNDITVVDTNGERLRTLQDKFD
                     LRVVQGHGSHPRVLREAGADDADMLVAVTSSDETNMVACQVAYSLFNTPNRIARIRSP
                     DYVRDADKLFHSDAVPIDHLIAPEQLVIDNIYRLIEYPGALQVVNFAEGKVSLAVVKA
                     YYGGPLIGNALSTMREHMPHIDTRVAAIFRHDRPIRPQGSTIVEAGDEVFFIAASQHI
                     RAVMSELQRLEKPYKRIMLVGGGNIGAGLARRLEKDYSVKLIERNQQRAAELAEKLQN
                     TIVFFGDASDQELLAEEHIDQVDLFIAVTNDDEANIMSAMLAKRMGAKKVMVLIQRRA
                     YVDLVQGSVIDIAISPQQATISALLSHVRKADIVGVSSLRRGVAEAIEAVAHGDESTS
                     RVVGRVIDEIKLPPGTIIGAVVRGNDVMIANDNLRIEQGDHVIMFLTDKKFITDVERL
                     FQPSPFFL"
     gene            complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OO357_003590"
     CDS             complement(1495..2784)
                     /gene="rsmB"
                     /locus_tag="OO357_003590"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HCR5888400.1"
                     /translation="MKKQRNLRSMAAQAVEQVVEQGQSLSNILPPLQQKVSDKDKALL
                     QELCFGVLRTLSQLDWLINKLMARPMTGKQRTVHYLIMVGLYQLLYTRIPPHAALAET
                     VEGAIAIKRPQLKGLINGVLRQFQRRQEELLAEFNTRDARYLHPSWLLKRLQKAYPEQ
                     WQSIAEANNQRPPMWLRINRTHHSRDSWLALLDEAGMKGFPHADYPDAVRLETPAPVH
                     ALPGFEDGWVTVQDASAQGCMTWLAPQNGEHILDLCAAPGGKTTHILEVAPEAQVVAV
                     DIDEQRLSRIYDNLKRLGMKATVKQGDGRYPSQWCGEQQFDRILLDAPCSATGVIRRH
                     PDIKWLRRDRDIPELAQLQSEILDAIWPHLKSGGTLVYATCSVLPEENSLQIKAFLQR
                     TADAELCETGTPEQPGKQNQPGAEEGDGFFYAKLIKK"
     gene            complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OO357_003591"
     CDS             complement(2830..3777)
                     /gene="fmt"
                     /locus_tag="OO357_003591"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HCR5888401.1"
                     /translation="MSESLRIIFAGTPDFAARHLDALLSSGHNVVGVFTQPDRPAGRG
                     KKLMPSPIKVLAEEEGLPVFQPVSLRPQENQQLVADLQADVMVVVAYGLILPKAVLEM
                     PRLGCINIHGSLLPRWRGAAPIQRSLWAGDAETGVTIMQMDVGLDTGDMLYKLSCPIT
                     AEDTSGTLYDKLAELGPQGLITTLKQLADGTAKPEVQDETLVTYAEKLSKEEARIDWS
                     LSAAQLERCIRAFNPWPMSWLEIEGQPVKVWKASVIDTATNAAPGTILEANKQGIQVA
                     TGDGILNLLSLQPAGKKAMSAQDLLNSRREWFVPGNRLV"
     gene            complement(3792..4301)
                     /gene="def"
                     /locus_tag="OO357_003592"
     CDS             complement(3792..4301)
                     /gene="def"
                     /locus_tag="OO357_003592"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HCR5888402.1"
                     /translation="MSVLQVLHIPDERLRKVAKPVEEVNAEIQRIVDDMFETMYAEEG
                     IGLAATQVDIHQRIIVIDVSENRDERLVLINPELLEKSGETGIEEGCLSIPEQRALVP
                     RAEKVKIRALDRDGKPFELEAEGLLAICIQHEMDHLVGKLFMDYLSPLKQQRIRQKVE
                     KLDRLKARA"
     gene            4466..5590
                     /gene="dprA"
                     /locus_tag="OO357_003593"
     CDS             4466..5590
                     /gene="dprA"
                     /locus_tag="OO357_003593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HCR5888403.1"
                     /translation="MVDIEIWLRLMSISSLYGDDMVRIAHWLAKQSHIDAVVLQQTGL
                     TLRQAQRFLSFPRKSIESSLCWLEQPNHHLIPADSEFYPPQLQATTDYPGALFVEGEL
                     HALHSFQLAVVGSRAHSWYGERWGRLFCETLATCGVTITSGLARGIDGVVHKAALQVN
                     GVSIAVLGNGLNTIHPRRHARLAASLFEQGGALVSEFPLDVPPLAYNFPRRNRIISGL
                     SKGVLVVEAALRSGSLVTARCALEQGREVFALPGPIGNPGSEGPHWLIKQGAILVTEP
                     EEILENLQFGLHWLPDAPENSFYSPDQQDVALPFPELLANVGDEVTPVDVVAERAGQP
                     VPEVVTQLLELELAGWIAAVPGGYVRLRRACHVRRTNVFV"
     gene            5562..6035
                     /locus_tag="OO357_003594"
     CDS             5562..6035
                     /locus_tag="OO357_003594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005134083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF494 domain-containing protein"
                     /protein_id="HCR5888404.1"
                     /translation="MFDVLMYLFETYIHTEAELRVDQDKLEQDLTDAGFEREDIYNAL
                     LWLEKLADYQEGLAEPMQLASDPLSMRIYTPEECERLDASCRGFLLFLEQIQVLNLET
                     REMVIERVLALDNAEFELDDLKWVILMVLFNIPGCENAYQQMEELLFEVNEGMLH"
     gene            6064..6606
                     /locus_tag="OO357_003595"
     CDS             6064..6606
                     /locus_tag="OO357_003595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_026210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
                     /protein_id="HCR5888405.1"
                     /translation="MAKSALFTVRNNESCPKCGAELVIRSGKHGPFLGCSQYPACDYV
                     RPLKSSADGHIVKVLEGQVCPVCGANLVLRQGRFGMFIGCSNYPECEHTELIDKPDET
                     AITCPQCRTGHLVQRRSRYGKTFHSCDRYPECQFAINFKPIAGECPECHYPLLIEKKT
                     AQGVKHFCASKQCGKPVSAE"
     gene            6611..7183
                     /gene="tsaC"
                     /locus_tag="OO357_003596"
     CDS             6611..7183
                     /gene="tsaC"
                     /locus_tag="OO357_003596"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709070.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase type 1
                     TsaC"
                     /protein_id="HCR5888406.1"
                     /translation="MNNNLQGDAIAAAIDVLNEKRVIAYPTEAVFGVGCDPDSETAVM
                     RLLELKQRPVDKGLILIAANYEQLKPYIDDTMLTDVQRETIFSCWPGPVTFVFPAPAT
                     TPRWLTGRFDSLAVRVTDHPLVVALCQAYGKPLVSTSANLSGLPPCRTVDEVRAQFGA
                     AFPVVPGETGGRLNPSEIRDALTGELFRQG"
     gene            7188..8006
                     /gene="aroE"
                     /locus_tag="OO357_003597"
     CDS             7188..8006
                     /gene="aroE"
                     /locus_tag="OO357_003597"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HCR5888407.1"
                     /translation="METYAVFGNPIAHSKSPFIHQQFAQQLNIEHPYGRVLAPINDFI
                     NTLNAFFRAGGKGANVTVPFKEEAFARADELTERAALAGAVNTLKRLEDGRLLGDNTD
                     GVGLLSDLERLSFIRPGLRILLIGAGGASRGVLLPLLSLDCAVTITNRTVSRAEELAK
                     LFAHTGSIQALGMDELEGHEFDLIINATSSGISGDIPAIPSSLIHPGIYCYDMFYQKG
                     KTPFLAWCEQRGSKRNADGLGMLVAQAAHAFLLWHGVLPDVEPVIKQLQEELSA"
     gene            8003..8260
                     /locus_tag="OO357_003598"
     CDS             8003..8260
                     /locus_tag="OO357_003598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1488 domain-containing protein"
                     /protein_id="HCR5888408.1"
                     /translation="MNQAIQFPDREEWDENKKCVCFPALVNGMQLTCAISGESLAYRF
                     TGDTPEQWLASFRQHRWDLEEEAENLIQEQSEDDQGWVWLP"
     gene            complement(8236..8790)
                     /locus_tag="OO357_003599"
     CDS             complement(8236..8790)
                     /locus_tag="OO357_003599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HCR5888409.1"
                     /translation="MSDVLRPYRDLFPQIGQRVMIDDSSVVIGDVRLADDVGIWPLVV
                     IRGDVHYVQIGARTNIQDGSMLHVTHKSSYNPDGNPLTIGEDVTVGHKVMLHGCTIGN
                     RVLVGMGSILLDGAIVEDDVMIGAGSLVPQNKRLESGYLYLGSPVKQIRPLSDEEKAG
                     LRYSANNYVKWKDEYLDQGNQTQP"
BASE COUNT         2259 a         2192 c         2252 g         2326 t
ORIGIN      
        1 ctaacaaatg ttaagccatt ttcctgccag gaatgaattt aattaatcct tgataatcaa
       61 agggaaataa taaggcgtca ttagacgcct tattaattac aagaagaaag ggcttggctg
      121 gaagagtctt tcgacgtcgg taataaactt tttatctgtg aggaacataa ttacgtgatc
      181 gccttgctca atgcgcagat tgtcattggc aatcatcacg tcgtttccac gtaccaccgc
      241 tccaataatc gttcctggcg gtagcttgat ttcgtcaatg actctgccga caacgcgtga
      301 ggtgctttca tcaccgtgag caacggcttc aatagcttct gctacgccgc ggcgcaatga
      361 ggaaacacca acaatatctg cttttcgcac atggctaagc aacgcagaaa tagttgcttg
      421 ttgtggtgaa attgcaatat cgatcacgct cccctgaacc agatccacat aagcgcgacg
      481 ctggatcaat accatcacct ttttcgcacc catacgtttg gcaagcatgg cggacatgat
      541 attggcctcg tcatcgttgg tgacggcaat aaacagatca acttgatcga tatgttcttc
      601 ggccagcagt tcttgatccg acgcatcacc aaaaaagacg atcgtattct gcaacttttc
      661 cgccagttcg gcagcgcgct gctgattacg ttcgatgagt ttaacgctgt aatctttttc
      721 cagacgacgc gccagccctg caccgatatt accgccacca accagcatga tccgtttata
      781 cggtttttcc agtcgctgta attcactcat caccgcgcgg atatgctgtg aagcggcaat
      841 aaagaacact tcatcaccag cttcaacaat ggtcgaacct tgtggacgaa tggggcgatc
      901 gtggcggaaa attgctgcca cacgagtatc gatatgtggc atatgttcgc gcatggtcga
      961 aagtgcatta ccaatcagcg ggccgccata ataggcttta actacagcga ggctgacttt
     1021 accctcagcg aagttcacca cctgcaatgc gcctggatac tcaatcagtc ggtaaatatt
     1081 atcgataacc aactgctctg gtgcgatcag atgatcaatc ggtacagcat ctgaatgaaa
     1141 tagcttatcg gcatcgcgta cgtagtctgg tgagcggatg cgagcgatgc gattaggggt
     1201 gttgaaaagt gagtaggcta cctggcaggc aaccatattg gtttcatctg aactggttac
     1261 agcaaccagc atatcggcgt cgtcggcacc tgcctcccgc aatacgcgtg gatgagagcc
     1321 atgcccctgc acgactcgca ggtcaaattt atcctgcaag gtccgcagac gctcaccgtt
     1381 ggtatcgaca acagtaatat cgttgttctc gccaaccagg ttttccgcca gtgtgccgcc
     1441 aacctggccg gcacccagaa tgataatttt catcagtcgc gacccgttat ctcatcactt
     1501 tttgattagc ttagcgtaaa agaagccgtc gccctcttcg gcaccaggct gattttgttt
     1561 acccggttgc tctggtgttc ctgtttcgca aagttcggca tcagcggtac gttgcaaaaa
     1621 ggctttaatc tgcaggctat tctcttccgg taacaccgaa caggtggcat agaccagggt
     1681 tccaccggat tttaaatgcg gccaaatggc gtcgagaatt tcagactgca attgcgcgag
     1741 ttccgggata tcgcgatcgc gacgtaacca tttaatatct ggatggcgac gaatcacccc
     1801 cgttgctgaa caaggcgcat ctaataaaat gcgatcaaac tgttgctcgc cacaccattg
     1861 ggaagggtaa cggccatcac cttgtttcac ggttgccttc ataccaaggc gttttaaatt
     1921 gtcgtaaatg cgagagaggc gctgttcgtc aatatcaacc gcaacaacct gcgcttctgg
     1981 tgccacctca aggatatgcg ttgttttacc gccgggggcg gcacaaagat ccaagatgtg
     2041 ttcaccgttt tgtggcgcaa gccaggtcat gcaaccttgt gctgatgcat cctgaacggt
     2101 aacccatccg tcttcaaaac caggtagcgc atgaacaggt gcaggtgttt ccagccgtac
     2161 agcatcaggg taatccgcat gcgggaaacc tttcattcct gcttcatcca gcaatgcaag
     2221 ccagctgtcg cgggaatgat gcgtacgatt aatacgcagc cacattggcg gacgctggtt
     2281 attggcttcg gcgatggatt gccactgctc tggatacgct ttttgcagac gcttcagcaa
     2341 ccaggaagga tgcagataac gtgcatcacg ggtattaaac tcggctaata actcttcttg
     2401 ccgacgctgg aactggcgta atacaccgtt aatcaaccct ttaagttgcg gacgcttaat
     2461 tgcgatagcg ccttcaaccg tttcagccag cgcagcatga ggtggaatgc gggtataaag
     2521 cagttgatac aaaccaacca taatcaggta atgcacagtg cgctgtttgc cggtcatcgg
     2581 acgggccatt aacttattaa tcagccagtc taactgcgaa agcgtacgca gtacgccaaa
     2641 gcacaactct tgaagaagtg ctttgtcttt atcggaaact ttttgctgga gcggtggcag
     2701 aatgttgctt aatgattgcc cttgctcgac gacttgttca acggcctggg ccgccatgct
     2761 acgtaaatta cgttgttttt tcataagtat aaaaatgctc ggcaagaccg ggcttagaag
     2821 agtggactat cagaccagac ggttgcccgg aacaaaccat tcccgacgag agttcaggag
     2881 gtcttgcgcg ctcatcgctt tcttacccgc aggttgtaac gagagcaggt tcaggatgcc
     2941 atcaccagtc gcaacctgaa tgccttgttt gttggcttca aggatcgttc ctggtgcagc
     3001 gttggttgcc gtatcaatga ccgacgcttt ccagacttta accggctgtc cttcaatttc
     3061 cagccagctc attggccatg ggttgaaagc gcgaatgcag cgttcaagct gtgctgccga
     3121 aagtgaccag tcaatacgcg cttcttcttt actcaacttc tcggcgtaag tgacaagagt
     3181 ttcgtcctga acttctggtt tcgccgtgcc gtctgccaat tgtttcaacg tggtgataag
     3241 cccttgtggg ccaagctctg ccagcttgtc gtacaacgta ccactggtat cttctgcagt
     3301 aatcgggcag gagagcttat agagcatgtc accggtgtct aaaccaacat ccatttgcat
     3361 aatggttaca ccagtttctg catcacccgc ccatagtgag cgttggattg gtgcagcacc
     3421 gcgccagcgt ggcagcagtg aaccatgaat gttgatacag ccaagacgcg gcatctccag
     3481 cacagcttta ggcagaatta aaccataggc gacgacgacc ataacatcag cctgcaaatc
     3541 ggcgaccagt tgctggtttt cttgtggacg cagggaaaca ggttgaaaaa cgggcagacc
     3601 ttcttcctca gccagaactt taatcgggct gggcatcagt tttttaccgc gtcctgccgg
     3661 tcggtctggc tgggtgaaca cgccaacgac gttatgacca gaagacaaca gcgcgtcgag
     3721 atgacgcgct gcaaagtcag gtgtacccgc aaaaataata cgtagtgatt ctgacacgtt
     3781 agttcttatc cttaagcccg ggctttcaga cgatccagtt tttcaacttt ctgacgaatt
     3841 cgttgttgtt tcagcggtga cagataatcc ataaacagtt tgccaaccag gtgatccatc
     3901 tcatgctgaa tacagatagc taacagaccc tctgcttcca gttcaaatgg tttaccgtcg
     3961 cggtcaaggg cgcgaatttt aactttctct gcgcgcggca ctaaagcacg ttgttcaggg
     4021 atcgacaggc aaccttcttc aatgcctgtt tcgccgcttt tttctaaaag ctccggattg
     4081 attaacacca gccgttcgtc acggttttcc gaaacatcaa taacaatgat acgttgatgg
     4141 atatcaacct gggttgccgc caggccaata ccttcttctg cgtacatcgt ctcgaacata
     4201 tcatcgacga tacgctgaat ttctgcattc acttcttcta ccggtttagc aactttgcga
     4261 agccgctcgt ccggaatatg taacacttgc aaaactgaca taaatctcca gagatgtgtt
     4321 caggagttag aaagattatt tcttctattc tagacaaatc cccctctgat tgacagcatc
     4381 acttgattga cagcatcact tgattgacag catcactgac caatcgcaaa gattgctaag
     4441 gcttgcttat ggcagggaga taaggatggt cgatatagaa atttggctgc gtttaatgag
     4501 catcagcagc ttgtacggcg atgatatggt ccgtatcgct cactggctgg caaaacagtc
     4561 gcatattgat gcggttgtat tgcagcaaac agggcttaca ttgcggcagg cacaacgctt
     4621 tctttcattt ccacgaaaga gtatcgaaag ctcactttgt tggttggagc aacccaacca
     4681 tcatttaatc cctgcggaca gcgaatttta tcctcctcaa cttcaggcga cgacagatta
     4741 ccccggcgca ctgtttgttg aaggagaact gcacgcgctg cattcatttc agcttgccgt
     4801 agtggggagt cgggcgcatt catggtatgg cgagcgatgg gggcgattat tttgcgaaac
     4861 tctggcgacg tgtggagtga caattacgag tggactggcg cgtggaatcg atggtgtggt
     4921 gcataaagcg gccttacagg taaatggcgt cagcattgct gtattgggga atggacttaa
     4981 taccattcat ccgcgtcgcc atgcccgact ggctgccagt ctgtttgaac agggtggcgc
     5041 tctcgtctcg gaatttcccc tcgatgttcc accccttgct tacaatttcc cacgaagaaa
     5101 tcgcattatc agtggtctaa gtaaaggtgt actggtggtg gaagcggctt tgcgcagtgg
     5161 ttcgctggtg acagcacgtt gtgcgcttga gcaggggcgt gaagtttttg ccttgccagg
     5221 tccaataggg aatccgggaa gcgaagggcc tcactggtta ataaaacaag gtgcgattct
     5281 tgtgacggaa ccggaagaaa ttctggaaaa cttgcaattt ggattgcact ggttgccaga
     5341 cgcccctgaa aattcatttt attcaccaga tcagcaagac gtggcattgc catttcctga
     5401 gctcctggct aacgtaggag atgaggtaac acctgttgac gtcgtcgctg aacgtgccgg
     5461 ccaacctgtg ccagaggtag ttactcaact actcgaactg gagttagcag gatggatcgc
     5521 agctgtaccc ggcggctatg tccgattgag gagggcatgc catgttcgac gtactaatgt
     5581 atttgtttga aacctatatt cacactgaag ctgagttgcg tgtggatcaa gacaaacttg
     5641 aacaggatct taccgacgca ggttttgagc gagaagatat ctacaatgcc ctgctatggc
     5701 tggaaaaact tgctgattat caggaagggt tggcagaacc gatgcaactg gcctcggatc
     5761 ctctctccat gcgtatttat acaccggaag agtgtgaacg actggatgcc agctgccgtg
     5821 ggtttctgct tttccttgag cagattcagg tgctcaacct tgaaactcgt gaaatggtga
     5881 tagagcgagt gctcgcgctg gataacgcag agttcgaact ggatgatctg aaatgggtga
     5941 tcctgatggt gttgttcaac atcccgggct gcgaaaatgc gtaccagcaa atggaagaat
     6001 tactctttga agtgaatgaa ggtatgctgc attaatcatt tctttaattc agcataagtt
     6061 gttatggcaa aatcagcact gttcacggtg cgtaataatg agtcctgccc aaagtgcggg
     6121 gctgaactgg ttattcgatc cgggaaacac ggtccgtttc ttggatgctc acagtatccg
     6181 gcgtgtgact acgtccgtcc tctgaaatct tcagcggatg gacatattgt caaagttctg
     6241 gaggggcagg tttgccccgt atgtggcgca aatctggtat tacgccaggg acgctttggt
     6301 atgtttattg gttgcagtaa ctaccctgaa tgcgaacata ccgaacttat cgataaaccg
     6361 gacgaaacag caattacatg cccccaatgt cggacgggcc atctggtcca gcgccgctcc
     6421 cgttatggca aaacatttca ctcctgtgat cgctacccgg agtgtcaatt tgccattaac
     6481 ttcaaaccaa tagctggaga atgccctgag tgtcattatc cgctactcat cgaaaagaaa
     6541 accgcgcagg gtgtaaaaca cttttgtgcc agtaaacaat gtggaaagcc ggtttcggcg
     6601 gaataataac gtgaataata acctgcaagg agacgctatc gcagctgcga tagatgtcct
     6661 caatgaaaaa cgtgtcatcg cctatccaac ggaagccgtt ttcggtgtcg ggtgcgatcc
     6721 tgatagcgaa acagcagtga tgagactgtt ggagttaaaa cagcgtccgg ttgataaggg
     6781 gctgatttta atcgcggcaa attacgagca gcttaaaccc tatattgatg acaccatgtt
     6841 gactgacgtg cagcgtgaaa ccattttttc ctgctggcca ggtcctgtca cctttgtctt
     6901 tcccgcgcct gcgacaacac cgcgctggtt gacgggccgc tttgattcgc ttgctgtacg
     6961 agtcaccgac catccgttgg tggttgcttt gtgccaggct tatggtaaac cgctggtttc
     7021 taccagtgcc aacttgagtg gattgccacc ttgtcgaaca gtagacgaag ttcgcgcaca
     7081 atttggcgcg gcgttcccgg ttgtgcctgg tgaaacgggg gggcgtttaa atccttcaga
     7141 aatccgcgat gccctgacgg gtgaactgtt tcgacagggg taacataatg gaaacctatg
     7201 ctgtttttgg taatccgata gcccacagca aatcgccatt cattcatcag caatttgctc
     7261 agcaactgaa tattgaacat ccctatgggc gcgtgttggc acccatcaat gatttcatca
     7321 acacactgaa cgctttcttt cgtgctggtg gtaaaggtgc gaatgtgacg gtgcctttta
     7381 aagaagaggc ttttgccaga gcggatgagc ttactgaacg ggcagcgttg gctggtgcag
     7441 ttaataccct caagcggtta gaagatggac gcctgctggg tgacaatacc gatggtgtag
     7501 gcttgttaag cgatctggaa cgtctgtctt ttatccgccc tggtttacgt attctgctta
     7561 tcggcgctgg tggagcatct cgcggcgtac tactgccact tctttccctg gactgtgcgg
     7621 tgacaatcac taatcggacg gtatcccgcg cggaagagtt ggctaaattg tttgcgcaca
     7681 ctggcagtat tcaggcgttg ggtatggacg aactggaagg tcatgagttt gatctcatta
     7741 ttaatgcaac atccagtggc atcagtggtg atattccggc gatcccgtca tcgctcattc
     7801 atccaggcat ttattgctat gacatgttct atcagaaagg aaaaactcct tttctggcat
     7861 ggtgtgagca gcgaggctca aagcgtaatg ctgatggttt aggaatgctg gtggcacagg
     7921 cggctcatgc ctttcttctc tggcacggtg ttctgcctga cgtagaacca gttataaagc
     7981 aattgcagga ggaattgtcc gcgtgaatca ggccatccag tttccggaca gggaagagtg
     8041 ggacgagaat aaaaaatgtg tatgttttcc cgctctcgtg aatggtatgc aactgacatg
     8101 cgcgatctct ggcgagagtc tggcgtatcg ctttactgga gatacgccag aacagtggtt
     8161 agcgagtttt cgtcagcatc gctgggacct ggaagaagaa gcggaaaact taattcagga
     8221 acaaagtgaa gatgatcaag gctgggtctg gttaccctga tccagatatt cgtccttcca
     8281 tttcacgtaa ttattcgcgg aatagcgtaa cccagccttc tcttcatcac ttaacgggcg
     8341 gatctgtttg acggggctac cgagatacag atatccgctc tccagccgtt tattttgtgg
     8401 gaccagacta cccgcaccaa tcatcacatc atcttctact attgcgccat caagtaaaat
     8461 tgagcccatc ccaaccaaaa ctcgattgcc aatggtgcag ccgtggagca tcaccttgtg
     8521 accaacagtg acatcttcgc caatggttaa tgggttgcca tctgggttgt acgaggattt
     8581 atgagtgaca tgcaacatac tgccatcctg gatattggtg cgtgctccga tctgtacata
     8641 atgtacatct ccacgaatca caacgagcgg ccagatcccc acatcatcag ccagacgaac
     8701 gtcaccaatc acgacactgc tatcgtcgat cattacgcgc tgaccgattt gtggaaaaag
     8761 atcgcggtat gggcgtaaaa catcagacat acttacctca gcaataaatg atttactaat
     8821 gactttgggg gcattattgg ccttgtgcaa gtcttttagt atgcaaaaaa gcaccgtttt
     8881 gtgtgcgatt gcagcaaaaa gggtgaaaaa acaacaaaca gaaaagaaga tcaaaaaaat
     8941 acttgtgcaa aatattggga tccctataat gcgcctccgt tgagacgaca acgtgaaaca
     9001 cttcacagga tggtcggaac aacgaagag
//
LOCUS       DAJTRC010000137         9003 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.137, whole genome shotgun sequence.
ACCESSION   DAJTRC010000137 DAJTRC010000000
VERSION     DAJTRC010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 9003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9003
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.137"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..923
                     /locus_tag="OO357_003600"
     CDS             <1..923
                     /locus_tag="OO357_003600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5888410.1"
                     /translation="GIGEAPFMPAGVKSITDWYAQKERGTALGIFNSSTVIGQAIAPP
                     ALVLMQLAWGWRTMFVIIGVAGILVGICWYAWYRNRAQFVLTDEERTYLSASVKPRPQ
                     LQFSEWLALFKHRTTWGMILGFSGVNYTGWLYIAWLPGYLQAEQGFSLAKTGWVAAIP
                     FLAAAVGMWVNGIVVDRLAKKGYDLAKTRKTAIVCGLMMSALGTLLVVQSSSPAQAVA
                     FISMALFCVHFAGTSAWGLVQVMVSETKVASIAGIQNFGSFVFASFAPIVTGWVVDTT
                     HSFNLALVIAACVTFTGALCYFFIVKDRIE"
     gene            1175..2092
                     /gene="oxyR"
                     /locus_tag="OO357_003601"
     CDS             1175..2092
                     /gene="oxyR"
                     /locus_tag="OO357_003601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016831323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator OxyR"
                     /protein_id="HCR5888411.1"
                     /translation="MNIRDLEYLVALAEHRHFRRAADSCHVSQPTLSGQIRKLEDELG
                     VMLLERTSRKVLFTQAGMLLVDQARTVLREVKVLKEMASQQGETMSGPLHIGLIPTVG
                     PYLLPHIIPMLHQTFPKLEMYLHEAQTHQLLAQLDSGKLDCVILALVKESEAFIEVPL
                     FDEPMLLAIYEDHPWANRECVLMADLAGEKLLMLEDGHCLRDQAMGFCFEAGADEDTH
                     FRATSLETLRNMVAAGSGITLLPALAVPPERKRDGVVYLPCIKPEPCRTIGLVYRPGS
                     PLRSRYEQLAEAIRARMDGHFDKVLKQAV"
     gene            complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="OO357_003602"
     CDS             complement(2075..3475)
                     /gene="sthA"
                     /locus_tag="OO357_003602"
                     /EC_number="1.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418397.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Si-specific NAD(P)(+) transhydrogenase"
                     /protein_id="HCR5888412.1"
                     /translation="MPHSYDYDAIVIGSGPGGEGAAMGLVKQGARVAVIERYQNVGGG
                     CTHWGTIPSKALRHAVSRIIEFNQNPLYSDHSRLLRSSFADILNHADNVINQQTRMRQ
                     GFYERNHCEILQGNARFVDEHTLALDCPDGSVETLTAEKFVIACGSRPYHPTDVDFTH
                     PRIYDSDSILSMHHEPRHVLIYGAGVIGCEYASIFRGMDVKVDLINTRDRLLAFLDQE
                     MSDSLSYHFWNSGVVIRHNEEYEKIESCDDGVIMHLKSGKKLKADCLLYANGRTGNTD
                     SLALQNIGLETDSRGQLKVNSMYQTAQPHVYAVGDVIGYPSLASAAYDQGRIAAQALV
                     KGEATAHLIEDIPTGIYTIPEISSVGKTEQQLTAMKVPYEVGRAQFKHLARAQIVGMN
                     VGTLKILFHRETKEILGIHCFGERAAEIIHIGQAIMEQKGGGNTIEYFVNTTFNYPTM
                     AEAYRVAALNGLNRLF"
     gene            3796..4446
                     /gene="fabR"
                     /locus_tag="OO357_003603"
     CDS             3796..4446
                     /gene="fabR"
                     /locus_tag="OO357_003603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional repressor FabR"
                     /protein_id="HCR5888413.1"
                     /translation="MMGVRAQQKEKTRRSLVEAAFSQLSAERSFASLSLREVAREAGI
                     APTSFYRHFRDVDELGLTMVDESGLMLRQLMRQARQRIAKGGSVIRTSVSTFMEFIGN
                     NPNAFRLLLRERSGTSAAFRAAVAREIQHFIAELADYLELENHMPRAFTEAQAEAMVT
                     IVFSAGAEALDVGVEQRRQLEERLVLQLRMISKGAYYWYRREQEKTAIIPGNVKDE"
     gene            4446..4805
                     /locus_tag="OO357_003604"
     CDS             4446..4805
                     /locus_tag="OO357_003604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YijD family membrane protein"
                     /protein_id="HCR5888414.1"
                     /translation="MKQANQDRGTLLLALVAGLSINGTFAALFSSIVPFSVFPIISLV
                     LTVYCLHQRYLNRTMSVGLPGLAAACFILGVLLYSTVVRAEYPDIGSNFFPAVLSVIM
                     VFWIGAKMRNRKQEVAE"
     gene            complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="OO357_003605"
     CDS             complement(4845..5945)
                     /gene="trmA"
                     /locus_tag="OO357_003605"
                     /EC_number="2.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (uridine(54)-C5)-methyltransferase TrmA"
                     /protein_id="HCR5888415.1"
                     /translation="MTPEHLPTEQYEAQLAEKVVRLQSMMAPFSDLVPEVFRSPVSHY
                     RMRAEFRIWHDGDDLYHIIFDQQTKSRIRVDSFPAASELINQLMTAMIAGVRNNPVLR
                     HKLFQIDYLTTLSNQAVVSLLYHKKLDDEWRQEAEALRDALRAQNLNVHLIGRATKTK
                     IALDQDYIDERLPVAGKEMIYRQVENSFTQPNAAMNIQMLEWALDVTKGSKGDLLELY
                     CGNGNFSLALARNFDRVLATEIAKPSVAAAQYNIAANHIDNVQIIRMAAEEFTQAMNG
                     VREFNRLQGIDLKSYQFETIFVDPPRSGLDSETEKMVQAYPRILYISCNPETLCKNLE
                     TLSQTHKVERLALFDQFPYTHHMECGVLLTAK"
     regulatory      6059..6249
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            6314..8158
                     /gene="btuB"
                     /locus_tag="OO357_003606"
     CDS             6314..8158
                     /gene="btuB"
                     /locus_tag="OO357_003606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709770.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent vitamin B12 receptor BtuB"
                     /protein_id="HCR5888416.1"
                     /translation="MIKKASLLTACSVTAFSAWAQDTSPDTLVVTAIRFEQPRSTVLA
                     PTTVVTRQDIDRWQSTSVNDVLRRLPGVDITQNGGSGQLSSIFIRGTNASHVLVLIDG
                     VRLNLAGVSGSADLSQFPIALVQRIEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEI
                     SAGWGSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFL
                     SKTLYGALEHNFTDAWSGFVRGYGYDNRTNYDAYYSPGSPLLDTRKLYSQSWDAGLRY
                     NGELIKSQLITSYSHSKDYNYDPHYGRYDSSATLDEMKQYTVQWANNVIVGHGSIGAG
                     VDWQKQTTTPGTGYVEDGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQ
                     TSAGWEFIEGYRFIASYGTSYKALNLGQLYGFYGNPNLDPEKSKQWEGAFEGLTAGVN
                     WRISGYRNDVSDLIDYDDHTLKYYNEGKARIKGVEATANFDTGPLTHTVSYDYVDARN
                     AITDTPLLRRAKQQVKYQLDWQLYDFDWGITYQYLGTRYDKDYSSYPYQTVKMGGVSL
                     WDLAVAYPVTSHLTVRGKIANLFDKDYETVYGYQTAGREYTLSGSYTF"
     gene            8103..8960
                     /gene="murI"
                     /locus_tag="OO357_003607"
     CDS             8103..8960
                     /gene="murI"
                     /locus_tag="OO357_003607"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HCR5888417.1"
                     /translation="MATKLQDGNTPCLAATPSEPRPTVLVFDSGVGGLSVYDEIRHLL
                     PDLHYIYAFDNVAFPYGEKSEAFIVERVVAIVTAVQERYPLALAVVACNTASTVSLPA
                     LREKFDFPVVGVVPAIKPAARLTANGIVGLLATRGTVKRSYTHELIARFANECQIEML
                     GSAEMVELAEAKLHGEDVSLDALKRILRPWLRMKEPPDTVVLGCTHFPLLQEELLQVL
                     PEGTRLVDSGAAIARRTAWLLEHEAPDAKSADANIAFCMAMTPEAEQLLPVLQRYGFE
                     TLEKLAVLG"
BASE COUNT         2046 a         2173 c         2434 g         2350 t
ORIGIN      
        1 tgggtattgg cgaagcgcca tttatgcctg cgggagtaaa gtcgatcacc gactggtatg
       61 cacaaaaaga acgcggcacg gcgctgggga tttttaactc atctaccgtt atcggtcagg
      121 ctatcgcgcc tcctgctctt gtattgatgc agctggcatg gggctggcgg acgatgttcg
      181 ttatcatcgg cgtggcaggg attctggttg ggatctgttg gtacgcgtgg tatcgcaacc
      241 gggcgcagtt tgtcctgact gacgaagaac ggacgtatct ctccgcgtcg gttaaaccgc
      301 gtccacagct gcaatttagc gagtggctgg cgctgtttaa gcatcggaca acctggggga
      361 tgattttagg tttctctggc gtcaattata ccggttggct ttacattgcg tggttacccg
      421 gttatttgca agccgaacaa ggtttcagtc tggcgaaaac cggttgggta gcggcgattc
      481 ctttcctcgc ggcggcggtc gggatgtggg ttaacggtat tgtggtcgat cggctggcga
      541 aaaaaggcta cgacctggcg aagacgcgta aaacggctat tgtctgcggt ttgatgatgt
      601 cggcattagg cacgttgctg gtcgtgcaat cttcctcgcc agctcaggcg gtagcgttta
      661 tctcaatggc gctgttctgc gtgcatttcg cgggaacgtc tgcatgggga ctggtgcagg
      721 tgatggtgtc agaaacaaaa gtggcttcca tcgccgggat tcaaaacttt ggcagttttg
      781 tttttgcttc ctttgctccg atcgtaaccg gttgggtagt ggataccaca cactcgttta
      841 atctggcgct ggttatcgcg gcctgtgtga cgttcaccgg ggcgctgtgt tacttcttta
      901 ttgtcaaaga tcgcattgag taatcgttac tatcaggctc tcttgctgtg ggcctgtaga
      961 ataaaaaaaa gcggatcctg gagatccgca aaagttcacg ttggctttag ttattcgagt
     1021 tgagaaactc tcgaaacggg cagtgacttc aagggttaaa agaggtgccg ctccgtttct
     1081 gtgagcaatt atcagtcaga atgcttgata gggataatcg ttcattgcta ttctacctat
     1141 cgccatgaac tatcgtggcg atggaggatg gataatgaat attcgtgatc ttgagtacct
     1201 ggtggcattg gctgaacacc gccattttcg gcgtgcggca gattcctgcc acgttagcca
     1261 gccgacgctt agcggccaaa ttcgtaagct ggaagatgag ctgggcgtga tgttgctgga
     1321 gcggaccagc cgtaaagtgt tgttcaccca ggcgggaatg ctgctggtgg atcaggcgcg
     1381 taccgtgctg cgtgaggtga aagtccttaa agagatggca agccagcagg gtgagacgat
     1441 gtccggaccg ctgcatattg gtttgattcc tacggttgga ccatacctgc taccgcatat
     1501 tatcccgatg ctgcaccaga catttccaaa gctggaaatg tatctgcatg aagcacagac
     1561 ccaccagtta ctggcgcaac tggacagcgg caaactcgat tgcgtgatcc tcgcgctggt
     1621 gaaagagagc gaagcattca ttgaagtgcc gttgtttgat gagccaatgt tgctggctat
     1681 ctatgaagat cacccgtggg cgaaccgcga atgcgtactg atggccgatc tggcagggga
     1741 aaaactgctg atgctggaag atggtcactg tttgcgcgat caggcaatgg gtttctgctt
     1801 tgaagccggg gcggatgaag atacacactt ccgcgcgacc agcctggaaa ctctgcgcaa
     1861 catggtggcg gcaggtagcg ggatcacttt attgccagcg ctggctgtgc cgccggagcg
     1921 caaacgcgat ggggttgttt atctgccatg cattaagccg gaaccatgcc gcactattgg
     1981 cctggtttat cgtcctggct caccgctgcg cagccgctat gagcagctgg cagaggccat
     2041 ccgcgcaaga atggatggcc atttcgataa agtgttaaaa caggcggttt aaaccgttta
     2101 acgcagctac ccgataggct tccgccatcg tcgggtagtt aaaggtggtg ttgacgaagt
     2161 actcaatagt gttgccgcca cctttctgtt ccataatcgc ctgaccgata tgaataattt
     2221 cggcagcgcg ctcgccaaag cagtgaatac ccagaatctc ttttgtttcc cgatggaaca
     2281 aaattttcag cgtgcccacg ttcatgccga cgatttgtgc acgtgccaga tgtttgaact
     2341 gggcgcggcc cacttcatac ggcactttca tcgcggtcag ctgctgttcg gttttgccca
     2401 cagagctgat ttccgggatg gtgtaaatgc cggtagggat atcttcaatc agatgtgcgg
     2461 tggcttcgcc ttttaccagc gcctgcgcgg caatgcgccc ctggtcatag gccgctgatg
     2521 ccaggctcgg ataaccaatc acgtcgccca ccgcgtaaac gtgcggctgt gcggtctgat
     2581 acatgctgtt gaccttcagc tgtccgcgac tgtcagtttc cagcccaatg ttctgtaacg
     2641 ccagcgaatc ggtattaccg gtacgaccgt tggcatagag caggcagtca gctttcagtt
     2701 ttttacccga cttcagatgc atgatcacac cgtcgtcaca gctttcgatc ttctcgtact
     2761 cttcgttgtg acgaatcact acgccactgt tccagaagtg gtaggagaga gaatctgaca
     2821 tctcttgatc gagaaatgcc agcaggcgat cgcgggtgtt gatcagatcc acttttacat
     2881 ccataccgcg gaagatcgac gcatattcac agccgatcac tccagcacca tagataagta
     2941 catggcgcgg ttcgtggtgc atgctaagaa ttgagtcgct gtcgtaaatg cgtggatggg
     3001 tgaaatcaac atctgttgga tgatatggac gagagccgca ggcaataaca aatttttcag
     3061 cggttagtgt ttcaacgctg ccgtccgggc aatccagcgc caacgtatgc tcgtcaacaa
     3121 agcgagcgtt cccctgcaat atttcacagt gattacgttc gtaaaatccc tgacgcatgc
     3181 gcgtttgttg attaatcacg ttatcggcat ggttaaggat atcggcaaaa gaagagcgga
     3241 gcagtcggga atggtcgctg tatagtgggt tttgattgaa ttctataatg cggctgacgg
     3301 cgtgacggag agctttcgac gggatggtgc cccagtgggt gcaaccgccg ccaacatttt
     3361 gataacgctc gataactgcg acgcgcgcac cttgcttaac caggcccatt gcagcgcctt
     3421 cgccgccggg gccggaacct attactatgg catcgtaatc gtaggaatgt ggcatggtag
     3481 ggcttacctg ttcttataca taaaagcaac agaatggtaa cattctatcg cgggtaagcc
     3541 aattgatccc cgtcatttat ctggctatat cctgagcggc ctttgctttg tctgtttctt
     3601 acttttgccc tgacgtttta ttggattttt atcgacgata ctctccgttt aagcggcagg
     3661 tttccgctgt acgtaaaaga accggccagt aaatatgttt tattgcgtta ccgttcattc
     3721 acaatactgg agcaatccag tatgttcatt ctctggtata gtgccagcag tacttttggc
     3781 aaggattcag acatcgtgat gggcgtaaga gcgcaacaaa aagaaaaaac ccgccgttcg
     3841 ctggtggaag ccgcatttag ccaattaagt gctgaacgca gcttcgccag cctgagtttg
     3901 cgtgaagtgg cgcgtgaagc gggcattgct cccacctctt tttatcggca tttccgcgac
     3961 gtagacgaac tgggtctgac catggttgat gagagcggtt taatgctacg ccaactcatg
     4021 cgccaggcgc gtcagcgtat cgccaaaggc gggagtgtga tccgtacctc ggtctccaca
     4081 tttatggagt tcatcggtaa taatcctaac gccttccggt tattattgcg ggaacgctcc
     4141 ggcacctccg ctgcgtttcg tgccgccgtt gcgcgtgaaa ttcagcactt cattgcggaa
     4201 cttgcggact atctggaact cgaaaaccat atgccgcgtg cgtttactga agcgcaagcc
     4261 gaagcaatgg tgacaattgt cttcagtgcg ggtgccgagg cgttggacgt cggcgtcgaa
     4321 caacgtcggc aattagaaga gcgactggta ctgcaactgc gaatgatttc gaaaggggct
     4381 tattactggt atcgccgtga acaagagaaa accgcaatta ttccgggaaa tgtgaaggac
     4441 gagtaatgaa acaagcaaat caagatagag gtacgctgct gctggcgttg gttgctggtt
     4501 tatcgattaa tggtactttc gcagcgctgt ttagctccat tgtgccattt tctgtattcc
     4561 cgattatttc cctggtgctg acggtttact gcctgcatca acgttatctt aatcgcacca
     4621 tgtcggtagg cttgccgggt ctggcagctg cctgttttat tctcggcgta ctgctgtaca
     4681 gcacggtagt tcgtgcggaa tatccggata tcggctctaa cttcttcccg gcagtactct
     4741 ccgtcattat ggtgttctgg attggcgcga agatgcgtaa ccgtaagcag gaagttgctg
     4801 agtaatcggt atcatgccgg atgctggcgc atccggcatg ggttttactt cgcggtcagt
     4861 aatacgccgc actccatatg gtgcgtgtag gggaactgat caaacagagc cagacgttcg
     4921 accttgtgcg tctggcttaa tgtttccaga tttttgcata acgtttccgg attgcaggag
     4981 atatacagaa tacgtggata cgcctgcacc attttctcgg tttcactgtc cagaccgctg
     5041 cgcggtgggt cgacaaaaat ggtttcgaac tgatagctct ttaagtcgat cccttgcagg
     5101 cggttaaact cgcgcacacc attcatcgcc tgagtaaatt cttctgccgc catacgaata
     5161 atttgtacgt tatcaatatg gttagcggca atgttgtatt gcgcggcagc gactgacggt
     5221 ttggcgattt cggtggctaa tacccgatca aaattgcgcg ccagcgctaa tgaaaagtta
     5281 ccgttgccgc agtacagctc cagtaaatcg ccttttgagc ctttggttac gtccagcgcc
     5341 cattccagca tctgaatatt catcgccgcg ttcggctggg taaagctgtt ttctacctga
     5401 cgatagatca tctctttccc tgcgaccggc agacgctcgt cgatgtaatc ttgatccagc
     5461 gcgattttgg ttttcgttgc ccgaccaatc agatgcacat tcagattctg cgcgcgcagt
     5521 gcatcgcgca gtgcttccgc ttcctgacgc cactcatcat ccagcttttt atggtacagc
     5581 agggaaacca ccgcctgatt actcagcgtg gtgaggtaat caatctggaa caacttgtgg
     5641 cgcagaacgg gattattacg cacacccgca atcatcgccg tcatcaactg gttgatgagt
     5701 tcactggcgg cggggaagct atccacgcgg atgcggcttt tggtttgttg atcgaaaatg
     5761 atgtgataca ggtcatcgcc atcgtgccag atgcggaact ccgcgcgcat ccggtaatga
     5821 ctgaccggcg agcgaaacac ttccggaacc aggtcagaaa acggtgccat catactttgc
     5881 aaacgtacca ctttttcggc taactgcgct tcatactgtt ctgttggaag gtgttcgggg
     5941 gtcatgatgt atcctgaaaa attaaagtac gcggcgattg tagggattgc tcatcagatg
     6001 tccagatctt gatgaattcc tatttgtgag ctacgtctgg acagtaactt gttacaacct
     6061 gtagcatcca cttgccggtc ctgtgagtta atagggaatc cagtgcgaat ctagagctga
     6121 cgcgcagcgg taaggaaagg tgcgatgatt gcgttatgcg gacactgcca tccggtggga
     6181 agtcatcatc tcttagtatc ttagataccc ctccaagccc gaagacctgc cggccaacgt
     6241 cgcatctggt tctcatcatc gcgtaatatt gatgaaacct gcggcatcct tcttctattg
     6301 tggatgcttt acaatgatta aaaaagcttc gctgctgacg gcgtgttccg tcacggcatt
     6361 ttccgcttgg gcacaggata ccagcccgga tactctcgtc gttactgcta tccgttttga
     6421 acagccgcgc agcactgtgc ttgcaccaac caccgttgtg acccgtcagg atatcgaccg
     6481 ctggcagtcg acctcggtca atgatgtgct gcgccgtctt ccgggcgtcg atatcaccca
     6541 aaacggcggt tcaggtcagc tctcatctat ttttattcgc ggtacaaatg ccagtcatgt
     6601 gctggtgtta attgatggcg tccgcctgaa tctggcgggg gtgagtggtt ctgccgacct
     6661 tagccagttc cctattgcgc ttgtccagcg tattgaatat atccgtggac cgcgctccgc
     6721 tgtttatggt tccgatgcaa taggcggggt ggtgaatatc atcacgacgc gcgatgaacc
     6781 cggaacggaa atttcagcag ggtggggaag caatagttat cagaactatg atgtctctac
     6841 gcagcaacaa ctgggggata aaacacgggt aacgctgttg ggcgattatg cccatactca
     6901 tggttatgat gttgttgcct atggtaatac cggaacgcaa gcgcagacag ataacgatgg
     6961 ttttttaagt aaaacgcttt atggggcgct ggagcataac tttactgatg cctggagcgg
     7021 ctttgttcgc ggctatggct atgataaccg taccaattat gacgcgtatt attctcccgg
     7081 ttcaccgttg ctcgataccc gtaaactcta tagccaaagt tgggacgccg ggctgcgcta
     7141 taacggcgaa ctgattaaat cacaactcat taccagctat agccatagca aagattacaa
     7201 ctacgatccc cattatggtc gttatgattc gtcggcgacg ctcgatgaga tgaagcaata
     7261 caccgtccag tgggcaaaca atgtcatcgt tggtcacggt agtattggtg cgggtgtcga
     7321 ctggcagaaa cagactacga cgccgggtac aggttatgtt gaggatggat atgatcaacg
     7381 taataccggc atctatctga ccgggctgca acaagtcggc gattttacct ttgaaggcgc
     7441 agcacgcagc gacgataact cacagtttgg tcgtcatgga acctggcaaa ccagcgccgg
     7501 ttgggaattc atcgaaggtt atcgcttcat tgcttcctac gggacatctt ataaggcact
     7561 aaatctgggg caactgtatg gcttctacgg aaatccgaat ctggacccgg agaaaagcaa
     7621 acagtgggaa ggcgcgtttg aaggcttaac cgctggggtg aactggcgta tttccggata
     7681 tcgtaacgat gtcagtgact tgatcgatta tgatgatcac accctgaaat attacaacga
     7741 agggaaagcg cggattaagg gtgtcgaggc gacagccaat tttgataccg gaccactgac
     7801 gcatactgtg agttatgatt atgtcgatgc gcgcaatgcg attaccgaca cgccgttgtt
     7861 acgccgtgct aaacagcagg tgaaatacca gctcgactgg cagttgtatg acttcgactg
     7921 gggtattact tatcagtatt taggcactcg ctatgataag gattactcat cttatcctta
     7981 tcaaaccgtt aaaatgggcg gtgtgagctt gtgggatctt gcggttgcgt atccggtcac
     8041 ctctcacctg acagttcgtg gtaaaatagc caacctgttc gacaaagatt atgagacagt
     8101 ctatggctac caaactgcag gacgggaata caccttgtct ggcagctaca ccttctgaac
     8161 cacgtcccac cgtgctggtg tttgactccg gcgtcggtgg gttgtcggtc tatgacgaga
     8221 tccggcatct cttaccggat ctccattaca tttatgcttt cgataacgtc gctttcccgt
     8281 atggcgaaaa aagcgaagcg tttattgttg agcgagtggt ggcaattgtc accgcggtgc
     8341 aagaacgtta tccccttgcg ctggctgtgg tcgcttgcaa caccgccagt accgtttcac
     8401 ttcctgcatt acgcgaaaag ttcgacttcc cggttgttgg tgtcgtgccg gcgattaaac
     8461 ctgctgcacg tctgacggca aatggcattg tcggattact ggcaacccgc ggaacagtta
     8521 aacgttctta tactcatgag ctgatcgcgc gtttcgctaa tgaatgccag atagaaatgc
     8581 tgggctcggc agagatggtt gagttggctg aagcgaagct gcatggcgaa gatgtttctc
     8641 tggatgcact aaaacgtatc ctacgcccgt ggttaagaat gaaagagccg ccagataccg
     8701 ttgtattggg ttgcacccat ttccctctac tacaagaaga actgttacaa gtgctgccag
     8761 agggaacccg gctggtggat tctggcgcag cgattgctcg ccgaacggcc tggttgttag
     8821 aacatgaagc cccggatgca aaatctgccg atgcgaatat tgccttttgt atggcaatga
     8881 cgccagaagc tgaacaatta ttgcccgttt tacagcgtta cggcttcgaa acgctcgaaa
     8941 aactggcagt tttaggctga tttggttgaa tgttgcgcgg tcggaaaatt attttaaatt
     9001 tcc
//
LOCUS       DAJTRC010000138         8852 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.138, whole genome shotgun sequence.
ACCESSION   DAJTRC010000138 DAJTRC010000000
VERSION     DAJTRC010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8852)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8852)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8852
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.138"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            74..226
                     /gene="hokE"
                     /locus_tag="OO357_003608"
     CDS             74..226
                     /gene="hokE"
                     /locus_tag="OO357_003608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5888418.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLAGDSLCEFTVKERNIEFRAVL
                     AYEPKK"
     gene            577..729
                     /gene="hokE"
                     /locus_tag="OO357_003609"
     CDS             577..729
                     /gene="hokE"
                     /locus_tag="OO357_003609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokE"
                     /protein_id="HCR5888419.1"
                     /translation="MLTKYALVAVIVLCLTVLGFTLLVGDSLCEFTVKERNIEFKAVL
                     AYEPKK"
     gene            complement(851..1471)
                     /gene="entD"
                     /locus_tag="OO357_003610"
     CDS             complement(851..1471)
                     /gene="entD"
                     /locus_tag="OO357_003610"
                     /EC_number="6.3.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit EntD"
                     /protein_id="HCR5888420.1"
                     /translation="MKTTHTSLPFAGHTLHFVEFDPANFCEQDLLWLPHYAQLQHAGR
                     KRKTEHLAGRIAAVYALREYGYKCVPAIGELRQPVWPAEVYGSISHCGATALAVVSRQ
                     PIGVDIEEIFSAQTATELTDNIITPAEHERLADCGLAFSLALTLAFSAKESAFKASEI
                     QTDAGFLDYQIISWNKQQVIIHRENEMFAVHWQIKEKIVITLCQHD"
     gene            complement(1646..3886)
                     /gene="fepA"
                     /locus_tag="OO357_003611"
     CDS             complement(1646..3886)
                     /gene="fepA"
                     /locus_tag="OO357_003611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore enterobactin receptor FepA"
                     /protein_id="HCR5888421.1"
                     /translation="MNKKIHSLALLVNLGIYGVAQAQEPTDTPVSHDDTIVVTAAEQN
                     LQAPGVSTITADEIRKNPVARDVSEIIRTMPGVNLTGNSTSGQRGNNRQIDIRGMGPE
                     NTLILIDGKPVSSRNSVRQGWRGERDTRGDTSWVPPEMIERIEVLRGPAAARYGNGAA
                     GGVVNIITKKGSGEWHGSWDAYFNAPEHKEEGATKRTNFSLTGPLGDEFSFRLYGNLD
                     KTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPLQSLELEAGYS
                     RQGNLYAGDTQNTNSDAYTRSKYGDETNRLYRQNYALTWNGGWDNGVTTSNWVQYEHT
                     RNSRIPEGLAGGTEGKFNEKATQDFVDIDLDDVMLHSEVNLPIDFLVNQTLTLGTEWN
                     QQRMKDLSSNTQALTGTNTGGAIDGVSATDRSPYSKAEIFSLFAENNMELTDSTIVTP
                     GLRFDHHSIVGNNWSPALNISQGLGDDFTLKIGIARAYKAPSLYQTNPNYILYSKGQG
                     CYASAGGCYLQGNDDLKVETSINKEIGLEFKRDGWLAGVTWFRNDYRNKIEAGYVAVG
                     QNAVGTDLYQWDNVPKAVVEGLEGSLNVPVSETVMWTNNITYMLKSENKTTGDRLSII
                     PEYTLNSTLSWQAREDLSMQTTFTWYGKQQPKKYNYKGQPAVGPETKEISPYSIVGLS
                     ATWDVTKNVSLTGGVDNLFDKRLWRAGNAQTTGDLAGANYIAGAGAYTYNEPGRTWYM
                     SINTHF"
     gene            4129..5331
                     /gene="fes"
                     /locus_tag="OO357_003612"
     CDS             4129..5331
                     /gene="fes"
                     /locus_tag="OO357_003612"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterochelin esterase"
                     /protein_id="HCR5888422.1"
                     /translation="MTALKVGSESWWQSKHGPEWQRLNDEMFEVTFWWRDPQGSEEYS
                     TIKRVWVYITGVTDHHQNSQPQSMQRIAGTDVWQWTTQLNANWRGSYCFIPTERDDIF
                     SAPSPDRLELREGWRKLLPQAIADPLNPQSWKGGLGHAVSALEMPQAPLQPGWDCPQA
                     PEIPAKEIIWKSERLKNSRRVWIFTTGDVTAEERPLAVLLDGEFWAQSMPVWPVLTSL
                     THRQQLPPAVYVLIDAIDTTHRAHELPCNADFWLAVQQELLPLVKVIAPFSDRADRTV
                     VAGQSFGGLSALYAGLHWPERFGCVLSQSGSYWWPHRGGQQEGVLLEKLKAGEVSAEG
                     LRIVLEAGIREPMIMRANQALYAQLHPIKESIFWRQVDGGHDALCWRGGLMQGLIDLW
                     QPLFHDRS"
     gene            5334..5552
                     /gene="ybdZ"
                     /locus_tag="OO357_003613"
     CDS             5334..5552
                     /gene="ybdZ"
                     /locus_tag="OO357_003613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin biosynthesis protein YbdZ"
                     /protein_id="HCR5888423.1"
                     /translation="MAFSNPFDDPQGAFYILRNAQGQFSLWPQQCALPAGWDIVCQPQ
                     SQESCQQWLEAHWRTLTPANFTQLQEAQ"
     gene            5549..>8852
                     /locus_tag="OO357_003614"
     CDS             5549..>8852
                     /locus_tag="OO357_003614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enterobactin synthase subunit F"
                     /protein_id="HCR5888424.1"
                     /translation="MSQHLPLVAAQPGIWMAEKLSDLPSAWSVAHYVELTGEVDAPLL
                     ARAVVAGLAQADTLRMRFTEDNGEVWQWVDDALIFELPEIIDLRTNIDPHGTAQALML
                     ADLQQDLRVDSGKPLVFHQLIQVADNRWYWYQRYHHLLVDGFSFPAITRQIANIYCAL
                     LRGEPTPASPFTPFADVVEEYQQYRESEAWQRDAAFWVEQRRQLPPPASLSPAPLAGR
                     SASADILRLKMEFTDGEFRQLATQLSGVQRTDLALALTALWLGRLCNRMDYAAGFIFM
                     RRLGSAALTATGPVLNVLPLGIHIAAQETLPELATRLATRLAAQLKKMRRHQRYDAEQ
                     IVRDSGRAAGDEPLFGPVLNIKVFDYQLDIPGVQAQTHTLATGPVNDLELALFPDEHG
                     DLSIEILANKQRYDEPTLIQHAERLKMLIAQFAADPALLCGDVDIMLPGEYAQLAQIN
                     ATQVEIPETTLSALVAEQAAKTPDAPALADARYLFSYREMHEQVVALANLLRERGVKP
                     GDSVAVALPRSVFLTLALHAIVEAGAAWLPLDTGYPDDRLKMMLEDARPSLLITTDDQ
                     LPRFSDVPNLTNLCYNAPLTPQGSAPLQLSQPHHTAYIIFTSGSTGRPKGVMVGQTAI
                     VNRLLWIQNHYPLTGEDVVAQKTPCSFDVSVWEFFWPFIAGAKLVMAEPEAHRDPLAM
                     QQFFAEYGVTTTHFVPSMLAAFVASLTPQTARQSCVTLKQVFCSGEALPADLCREWQQ
                     LTGAPLHNLYGPTEAAVDVSWYPAFGEELAQVRGSSVPIGYPVWNTGLRILDAMMHPV
                     PPGVAGDLYLTGIQLAQGYLGRPDLTASRFIADPFAPGERMYRTGDVARWLDNGAVEY
                     LGRSDDQLKIRGQRIELGEIDRVMQALPDVKQAVTHACVINQAAATGGDARQLVGYLV
                     SQSGLPLDTSALQAQLRETLPPHMVPVVLLQLPQLPLSANGKLDRKALPLPELKAQAP
                     GRAPKAGSETIIAAAFASLLGCDVQDADADFFALGGHSLLAMKLAAQLSRQFARQVTP
                     GQVMVASTVAKLATIIDGEEDSSRRMGFETILPLREGNGPTLFCFHPASGFAWQFSVL
                     SRYLDPQWSIIGI"
BASE COUNT         1917 a         2336 c         2432 g         2167 t
ORIGIN      
        1 acaccctggg gctgccactc caaaccagac aatttggatg gtagtccctt cttcgcatgg
       61 aggtaatata aacatgctga cgaaatatgc ccttgtggca gtcatagtgc tgtgtttgac
      121 ggtactggga tttacgcttc tggccggaga ctcgctgtgt gagtttacgg tgaaggaacg
      181 taatattgag ttcagggctg ttctcgctta cgaaccgaag aagtagccgt tgtgcgggga
      241 gtaatccccg catatccggt tgtcaggtca ggatggtaag gcacctgctt tacactttcg
      301 cccgcggtca gtgatggctg cgggcgattt cgttttaaga gcaatgatga gttttgccga
      361 cagaagtccg aaacaggact cactgtataa ataaacagct attttgctaa ggaagggtaa
      421 gataacggcg ggtgcctgaa gctttccggt ttcaggttaa ctctgaggtt tggaaagatg
      481 aagccccaag agatatttct atcaatcctg gggctgccac tccgaacctc aacaactcgg
      541 atggtagtcc cttcttcgca tggaggcaat ataaacatgc tgacgaaata tgcccttgtg
      601 gcagtcatag tgctgtgttt gacggtgctg ggatttacgc ttctggtcgg agactcgctg
      661 tgtgagttca ccgtaaagga acgtaatatt gagtttaagg ctgttcttgc ttacgaaccg
      721 aagaagtagc cgttgtgcgg ggagtaatcc ccgcatatcc ggttgtcagg tcagagtggt
      781 aaggcacctg ctttacactt tcgcccgcag tcagtgatgg ctgcgggcga atcgtaccag
      841 atgttgtcaa ttaatcgtgt tggcacagag ttatgactat tttttctttt atctgccagt
      901 gcacagcaaa catctcattc tcacgatgaa tgatgacctg ctgtttattc cagctaatta
      961 tctgatagtc cagaaaacct gcatcagttt ggatctcact tgccttaaat gcgctctctt
     1021 tggcggaaaa tgcaagtgtc agcgccagag aaaaggctaa accgcagtct gcgagtcgct
     1081 cgtgttctgc tggtgtaata atgttgtctg tcaattcggt cgctgtttgc gcagaaaaaa
     1141 tttcttcgat atcaacgcca atcggttgac gggaaaccac ggccaatgcc gtagccccgc
     1201 agtggctaat actgccgtat acctccgcag gccacaccgg ttggcgtagc tcgccgattg
     1261 cgggcacaca tttatagcca tattcccgca aggcataaac agcagcgatc cgtccggcta
     1321 aatgctctgt tttacgttta cgtccagcgt gttgcagttg tgcgtagtgc ggcagccaga
     1381 gtaaatcctg ctcacaaaaa ttcgccggat cgaactcaac aaaatgcagc gtatgtccgg
     1441 caaaggggag ggaggtatgc gtagttttca tatcgaccat attcgagact gatgacaaac
     1501 gcaaaactgc ctgatgcgct acgcttatca ggcctacgtg atcctgcaat atattgaatt
     1561 ggcaagatct ttgtaggccg gataaggcgt tcacgccgca tccggcgtga acgacgcgca
     1621 ctttgtcaac aatctgacgt tagcatcaga agtgagtatt aatgctcata taccacgtac
     1681 gtcccggctc gttataggta tacgcgccgg caccggcgat atagttggct cctgccagat
     1741 cgcccgtggt ctgggcatta cctgcacgcc acaaacgttt gtcgaacagg ttgtccacgc
     1801 cgccggtcag actgacattc ttcgtcacat cccaggtcgc gctcagaccc acaatgctgt
     1861 aaggactgat ttctttggtt tccgggccaa ccgctggctg acctttatag ttgtacttct
     1921 tcggctgctg cttgccgtac caggtgaagg tcgtttgcat cgacaaatct tcccgtgcct
     1981 gccagctcag cgttgagttc aacgtgtact ccgggatgat cgacaaacgg tcgcccgtgg
     2041 ttttgttttc actcttcagc atataagtga tgttattggt ccacatcacc gtttcgctaa
     2101 ctggtacgtt taacgatcct tccagacctt caaccactgc tttcggcacg ttatcccact
     2161 gatagagatc agtgccgact gcgttttgcc ctacagccac atagcccgct tcaatcttat
     2221 tgcgataatc gttacggaac caggtgacgc ccgccagcca gccgtcgcgt ttgaactcca
     2281 gaccaatctc tttgttgatg ctggtttcta ctttcaggtc atcgttacct tgcagatagc
     2341 agccgcccgc gctggcatag cagccctgac ctttactgta gagaatgtag ttcgggttag
     2401 tctggtatag gctcggcgct ttataggcgc gggcaatgcc tattttcagc gtgaagtcat
     2461 cgcctaaacc ttgcgatatg ttcagcgccg ggctccagtt attgccgaca atactgtgat
     2521 gatcgaaacg cagccccggc gttacgatgg tgctgtcagt cagctccatg ttgttttcgg
     2581 caaacagcga gaaaatttct gcttttgaat acgggctacg gtcggtggca ctcacgccat
     2641 caatagcgcc accggtattc gttccggtca gtgcttgggt gttggaactt aagtccttca
     2701 tccgttgctg attccactcc gtacccagcg tcagcgtctg gttaacgagg aaatcaatcg
     2761 gcaggttaac ttcgctgtgc agcatcacgt catcgagatc gatatcgacg aaatcctgtg
     2821 tcgctttttc gttaaatttc ccttcggtac cgcccgccag accttccgga atacgcgagt
     2881 tacgggtgtg ttcatactgc acccagttgc tggtggtcac gccgttatcc cagccaccgt
     2941 tccaggtcag cgcgtagttc tggcgataca ggcggttggt ttcatcgcca tatttcgagc
     3001 gagtgtaagc gtcagagttg gtgttctggg tatcgcccgc atacaggtta ccctggcggc
     3061 tgtaacccgc ttccagttcc agcgattgca atggtgcgaa gtcccagcgc accacgccgt
     3121 taatatcttt attgatcacc ccttcgcgcc cggctggtaa cgtcgtggca tacgttccgg
     3181 cacgcgcgga ctgatggccc tggttgatat cccacgcgtc agcctgagtt ttgtcgaggt
     3241 tgccatacaa gcggaagctg aattcgtcgc ccagcggacc ggtcaggcta aagttagtgc
     3301 gtttggtggc accttcctct ttatgttctg gcgcattgaa atatgcgtcc caggagccgt
     3361 gccactcgcc gctgcctttt ttggtaatga tgttaaccac gccgcccgcc gcgccgttgc
     3421 cataacgcgc agctgccgga ccacgcagaa cttcaatacg ttcaatcatt tcaggtggca
     3481 cccaggaggt atcaccacgg gtatcgcgct cgccacgcca gccctgacgc accgagttac
     3541 ggctgcttac cggcttgccg tcaatcaaaa tcagcgtgtt ttccggaccc ataccgcgaa
     3601 tatcaatctg tcggttattc ccacgctgac cactggtgga gttaccggtc aggttaacgc
     3661 ctggcatggt acggatgatc tccgacacat cgcgggcaac cgggtttttg cggatttcat
     3721 ctgcggtgat ggtcgaaacg ccaggagcct gcaagttctg ctcggcggcg gtaacgacaa
     3781 tggtatcgtc atgtgaaaca ggtgtatcgg tcggctcttg tgcctgcgct accccataaa
     3841 tccccagatt gaccaacaag gccagggaat gaatcttctt gttcattgtt ttattcctgc
     3901 atttttgcca cgaattgcaa ctgtcgggca tggtcgtcat caacacgacg catcccgtta
     3961 ccgcgaaaac ctttgatcct gaaagacacg cagtgcagtt ggttaattaa tgtccgcgct
     4021 tcccacggcg cgccattacg ctattgcaaa tgcaaatagt tattaataac attatcaata
     4081 tatttctgca atcaatgaaa aattgcacag taaacatggg gttatggtgt gacggcgtta
     4141 aaagtaggaa gtgagagctg gtggcagtcg aaacatggcc cggaatggca gcgtctgaat
     4201 gacgaaatgt ttgaggtcac tttctggtgg cgtgatcccc aaggttctga agaatactcg
     4261 acgataaagc gcgtatgggt ctacatcact ggtgtgaccg atcatcatca gaacagccag
     4321 ccccagtcga tgcagcgaat tgcaggtact gatgtctggc agtggacgac acaactcaat
     4381 gccaactggc gcggcagcta ctgctttatt cccaccgaac gcgatgacat tttttctgca
     4441 ccatcccccg atcgcctcga attacgtgaa ggctggcgaa aactattacc ccaggcaata
     4501 gccgatccgc tgaatccaca aagctggaaa ggcgggctag ggcacgctgt ttctgcactc
     4561 gaaatgccgc aagcgcctct gcaaccggga tgggattgtc cgcaagcgcc agaaatacca
     4621 gccaaagaaa ttatctggaa aagtgaacgg ttgaaaaatt cacgtcgtgt atggattttt
     4681 accaccggcg atgtaacagc agaagaacgc ccgctggcag ttttgctcga tggcgaattt
     4741 tgggcgcaaa gtatgcccgt ctggcctgtg ctgacttcgc tgacccatcg tcagcaactt
     4801 cctcccgccg tgtatgtgtt gatcgacgct atcgacacca cgcaccgcgc ccacgaactg
     4861 ccgtgtaatg cggatttctg gctggcagta cagcaagagt tattacccct ggtgaaagtt
     4921 attgcccctt ttagcgatcg cgccgatcgc accgtggtcg ccgggcagag ttttggtggg
     4981 ctgtccgcgc tgtatgccgg actgcactgg cctgaacgct ttggctgtgt attaagccag
     5041 tcaggatcgt actggtggcc gcatcggggc gggcagcaag agggcgtgtt acttgaaaag
     5101 ctaaaagctg gtgaagttag cgccgaaggt ctgcgcattg tgctggaagc gggtattcgc
     5161 gagccgatga tcatgcgggc caatcaggcg ctgtatgcgc aattacaccc tataaaagaa
     5221 tccattttct ggcgtcaggt tgacggcgga catgatgcgc tttgttggcg cggtggcttg
     5281 atgcaggggt taatcgacct ctggcaacca cttttccatg acaggagttg aatatggcat
     5341 tcagtaatcc cttcgatgat ccacagggag cgttttacat tttgcgcaat gcgcaggggc
     5401 aattcagtct gtggccgcaa caatgcgcct taccggcagg ctgggacatt gtgtgtcagc
     5461 cgcagtcaca agagtcctgc cagcagtggc ttgaagccca ctggcgtact ctgacaccgg
     5521 cgaatttcac ccagttgcag gaggcacaat gagccagcat ttacctttgg tcgccgcaca
     5581 gcccggcatc tggatggcag aaaaactgtc ggatttaccc tccgcctgga gcgtggcgca
     5641 ttacgttgag ttaaccggag aggttgatgc gccattactg gcccgcgcgg tggttgccgg
     5701 attagcgcaa gcagatactt tgcggatgcg ttttacggaa gataacggcg aagtctggca
     5761 atgggtcgat gatgcgctga tattcgaact gccagaaatt atcgacctgc gaaccaatat
     5821 tgatccgcac ggtactgcgc aggcattaat gctggcggat ttgcaacaag atttgcgcgt
     5881 cgatagcggt aaaccactgg tctttcatca actgatacag gtggcagata accgctggta
     5941 ctggtatcag cgttatcacc atttactggt cgatggcttc agttttccgg ccattacccg
     6001 ccagatcgcc aacatttact gcgcattgct gcgtggcgaa ccaacgcctg cttcgccgtt
     6061 tacgcctttc gctgatgtag tggaagagta ccagcaatac cgcgaaagcg aagcctggca
     6121 gcgtgatgcg gcattctggg tggaacagcg tcgccaactg ccaccgcccg cgtcactttc
     6181 tccggcacct ttagcggggc gcagcgcctc ggcagatatt ttgcgtctga aaatggaatt
     6241 taccgacggg gaattccgcc agctggctac gcaactttca ggtgtgcagc gtaccgattt
     6301 agcccttgcg ctgacggctt tgtggctggg gcgattgtgc aatcgcatgg actacgccgc
     6361 cggatttatc tttatgcgtc gactgggctc ggcggcgctg acggctaccg gacccgtgct
     6421 caacgttttg ccgctgggta ttcatattgc ggcgcaagaa acgctgccgg aactggcaac
     6481 ccgactggca acccgactgg cagcacaact gaaaaaaatg cgtcgtcatc aacgttacga
     6541 tgccgaacaa attgtccgtg acagcgggcg agcggcaggt gatgaaccgc tgtttggtcc
     6601 ggtactcaat attaaggtat ttgattacca actggatatt cctggtgttc aggcgcaaac
     6661 ccataccctg gcaaccggtc cggttaatga ccttgaactg gccctgttcc cggatgaaca
     6721 cggtgatttg agtattgaga tcctcgccaa taaacagcgt tacgatgagc caacgttaat
     6781 ccagcatgct gaacgcctga aaatgctgat tgcccagttc gccgcggatc cggcgctgtt
     6841 gtgcggcgat gtcgatatta tgctgccagg tgagtatgcg cagctggcgc agatcaacgc
     6901 cactcaggtt gagattccag aaaccacgct gagcgcgctg gtggcagaac aagcggcaaa
     6961 aacaccggat gctccggcgc tggcagatgc gcgttacctg ttcagctatc gggaaatgca
     7021 cgagcaggtg gtggcgctgg cgaatctgct gcgtgagcgc ggcgttaaac caggggacag
     7081 cgtggcggtg gcactaccgc gctcggtctt tttgaccctg gcactccatg caatagttga
     7141 agctggcgcg gcctggctac cgctggatac cggctatccg gacgatcgcc tgaaaatgat
     7201 gctggaagat gcgcgtccgt cgctgttaat taccaccgac gatcaactgc cgcgctttag
     7261 cgatgttccc aatttaacca acctttgcta taacgccccg cttacccccc agggcagtgc
     7321 gccgctgcaa ctttcacaac cgcaccacac ggcttatatc atctttacct ctggctccac
     7381 cggcaggccg aaaggggtaa tggtcgggca gacggctatc gtcaaccgcc tgttgtggat
     7441 tcaaaatcat tatccgctca caggcgaaga tgtcgttgcc caaaaaacgc cgtgcagttt
     7501 tgatgtctcg gtgtgggagt ttttctggcc gtttattgcc ggggctaaac tggtgatggc
     7561 tgaaccggaa gcgcaccgcg acccgctcgc tatgcagcaa ttctttgccg aatatggcgt
     7621 aacgaccacg cactttgtgc cgtcgatgct ggcagcattt gttgcctcgc tgactccgca
     7681 aaccgctcgc cagagttgcg tgacgttgaa acaggttttc tgtagtggtg aggccttacc
     7741 ggctgattta tgccgcgaat ggcaacagtt aaccggcgcg ccgttgcata acctgtatgg
     7801 cccgacggaa gcggcggtag atgtcagctg gtatccggct tttggcgagg aactggcaca
     7861 ggtgcgcggc agcagtgtgc cgattggtta tccggtatgg aatacgggtc tgcgtattct
     7921 tgatgcgatg atgcatccgg tgccgccggg tgtggcgggt gatctctatc tcaccggtat
     7981 tcaactggcg caggggtatc ttggacgacc cgatctgacc gccagccgct ttattgccga
     8041 tccttttgct cctggtgaac ggatgtatcg taccggagac gttgcccgct ggctggataa
     8101 cggcgcggtg gagtacctcg ggcgcagtga cgatcagcta aaaattcgcg ggcagcgtat
     8161 cgaactgggc gaaatcgatc gcgtgatgca ggcgctgccg gatgtcaaac aagccgttac
     8221 ccacgcctgt gtgattaacc aggcggcagc cactggtggt gatgcgcgtc agttggtggg
     8281 ctatctggta tcgcaatcgg gcctgccgtt ggataccagc gcattgcagg cgcagcttcg
     8341 cgaaacattg ccgccgcata tggtgccagt cgttctgctg caactgccgc agttgccact
     8401 tagcgccaac ggcaagctgg atcgcaaagc cttaccgttg cctgaactga aggcacaagc
     8461 gccagggcgt gcgccgaaag cgggcagtga aacgattatc gccgcggcat tcgcgtcgtt
     8521 gctgggttgt gacgtgcagg atgccgatgc tgatttcttt gcgctgggcg gtcattcgct
     8581 actggcaatg aaactggcag cgcagttaag tcgacagttt gctcgtcagg tgacgccggg
     8641 gcaggtgatg gtcgcgtcaa ccgtcgccaa actggcaacg attattgatg gtgaagagga
     8701 cagctcccgg cgcatgggat tcgaaaccat tctgccgttg cgtgaaggta atggcccgac
     8761 gctgttttgt ttccatccgg cgtccggttt tgcctggcag tttagcgtgc tctcgcgtta
     8821 tctcgatcca caatggtcga ttattggcat tc
//
LOCUS       DAJTRC010000139         8798 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.139, whole genome shotgun sequence.
ACCESSION   DAJTRC010000139 DAJTRC010000000
VERSION     DAJTRC010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8798)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8798)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8798
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.139"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..387
                     /gene="pdxH"
                     /locus_tag="OO357_003615"
     CDS             <1..387
                     /gene="pdxH"
                     /locus_tag="OO357_003615"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR5888425.1"
                     /translation="HQIENNPRVSLLFPWHTLERQVMVIGKAERLSTLEVMKYFHSRP
                     RDSQIGAWVSKQSSRISARGILESKFLELKQKFQQGEVPLPSFWGGFRVSLEQIEFWQ
                     GGEHRLHDRFLYQRENDAWKIDRLAP"
     gene            516..1790
                     /gene="tyrS"
                     /locus_tag="OO357_003616"
     CDS             516..1790
                     /gene="tyrS"
                     /locus_tag="OO357_003616"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HCR5888426.1"
                     /translation="MASSNLIKQLQERGLVAQVTDEEALAERLAQGPIALYCGFDPTA
                     DSLHLGHLVPLLCLKRFQQAGHKPVALVGGATGLIGDPSFKAAERKLNTEETVQEWVD
                     KIRKQVAPFLDFDCGENSAIAANNYDWFGNMNVLTFLRDIGKHFSVNQMINKEAVKQR
                     LNREDQGISFTEFSYNLLQGYDFACLNKQYDVVLQIGGSDQWGNITSGIDLTRRLHQN
                     QVFGLTVPLITKADGTKFGKTEGGAVWLDPKKTSPYKFYQFWINTADADVYRFLKFFT
                     FMSIEEINALEEEDKNSGKAPRAQYVLAEQVTRLVHGEEGLQAAKRITECLFSGSLSA
                     LSEADFEQLAQDGVPMVEMEKGADLMQALVDSELQPSRGQARKTIASNAITINGEKQS
                     DPEYFFKEEDRLFGRFTLLRRGKKNYCLICWK"
     gene            1849..2712
                     /gene="pdxY"
                     /locus_tag="OO357_003617"
     CDS             1849..2712
                     /gene="pdxY"
                     /locus_tag="OO357_003617"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase"
                     /protein_id="HCR5888427.1"
                     /translation="MMKNILAIQSHVVYGHAGNSAAEFPMRRLGANVWPLNTVQFSNH
                     TQYGKWTGCVMPPSHLTEIVQGIAAIDKLHTCDAVLSGYLGSAEQGEHILGIVRQVKA
                     ANPQAKYFCDPVMGHPEKGCIVAPGVAEFHVRHGLPASDIIAPNLVELEILCEHAVNN
                     VEEAVLAARELIEQGPQIVLVKHLARAGYSRDRFEMLLVTADEVWHISRPLVDFGMRQ
                     PVGVGDVTSGLLLVKLLQGATLQEALEHVTAAVYEIMVTTKAMQEYELQVVAAQDRIA
                     KPEHYFSATKL"
     gene            complement(2756..3361)
                     /gene="gstA"
                     /locus_tag="OO357_003618"
     CDS             complement(2756..3361)
                     /gene="gstA"
                     /locus_tag="OO357_003618"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione transferase GstA"
                     /protein_id="HCR5888428.1"
                     /translation="MKLFYKPGACSLASHITLRESGKDFTLVSVDLMKKRLENGDDYF
                     SVNPKGQVPALLLDDGTLLTEGVAIMQYLADSVPDRQLLAPVNSISRYKTIEWLNYIA
                     TELHKGFTPLFRPDTPEEYKPTVRAQLDKKLQYVNEALKDEHWICGQRFTIADAYLFT
                     VLRWAYAVKLNLEGLEHIAAFMQRMAERPEVQDALSAEGLK"
     gene            complement(3467..4969)
                     /gene="dtpA"
                     /locus_tag="OO357_003619"
     CDS             complement(3467..4969)
                     /gene="dtpA"
                     /locus_tag="OO357_003619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide/tripeptide permease DtpA"
                     /protein_id="HCR5888429.1"
                     /translation="MSTANQKPTESVSLNAFKQPKAFYLIFSIELWERFGYYGLQGIM
                     AVYLVKQLGMSEADSITLFSSFSALVYGLVAIGGWLGDKVLGTKRVIMLGAIVLAIGY
                     ALVAWSGHDAGIVYMGMAAIAVGNGLFKANPSSLLSTCYEKNDPRLDGAFTMYYMSVN
                     IGSFFSMIATPWLAAKYGWSVAFALSVVGLLITIVNFAFCQRWVKQYGSKPDFEPVNY
                     RNLLLTIIGVVALIAVATWLLHNQEVARMALGVVAFGIVVIFGKEAFAMKGAARRKMI
                     VAFILMLEAIIFFVLYSQMPTSLNFFAIRNVEHSILGLAVEPEQYQALNPFWIIIGSP
                     ILAAIYNKMGDTLPMPTKFAIGMVMCSGAFLILPLGAKFASDAGIVSVSWLVASYGLQ
                     SIGELMISGLGLAMVAQLVPQRLMGFIMGSWFLTTAGANLIGGYVAGMMAVPDNVTDP
                     LMSLEVYGRVFLQIGVATAVIAVLMLLTAPKLHRMTQDDAADKAAKATVA"
     gene            complement(5580..6215)
                     /gene="nth"
                     /locus_tag="OO357_003620"
     CDS             complement(5580..6215)
                     /gene="nth"
                     /locus_tag="OO357_003620"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HCR5888430.1"
                     /translation="MNKAKRLEILTRLRENNPHPTTELNFSSPFELLIAVLLSAQATD
                     ISVNKATAKLYPVANTPAAMLELGVEGVKTYIKTIGLYNSKAENIIKTCRILLEQHNG
                     EVPEDRAALEALPGVGRKTANVVLNTAFGWPTIAVDTHIFRVCNRTQFAPGKNVEQVE
                     EKLLKVVPAEFKVDCHHWLILHGRYTCIARKPRCGSCIIEDLCEYKEKVDI"
     gene            complement(6215..6910)
                     /gene="rsxE"
                     /locus_tag="OO357_003621"
     CDS             complement(6215..6910)
                     /gene="rsxE"
                     /locus_tag="OO357_003621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxE"
                     /protein_id="HCR5888431.1"
                     /translation="MSEIKDVIVQGLWKNNSALVQLLGLCPLLAVTSTATNALGLGLA
                     TTLVLTLTNLTISTLRHWTPAEIRIPIYVMIIASVVSAVQMLINAYAFGLYQSLGIFI
                     PLIVTNCIVVGRAEAFAAKKGPALSALDGFSIGTGATCAMFVLGSLREIIGNGTLFDG
                     ADALLGSWAKVLRVEIFHTDSPFLLAMLPPGAFIGLGLMLAGKYLIDERMKKRRAEAA
                     AERALPNGETGNV"
     gene            complement(6914..7534)
                     /gene="rsxG"
                     /locus_tag="OO357_003622"
     CDS             complement(6914..7534)
                     /gene="rsxG"
                     /locus_tag="OO357_003622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxG"
                     /protein_id="HCR5888432.1"
                     /translation="MLKTIRKHGITLALFAAGSTGLTAAINQMTKTTIAEQASLQQKT
                     LFDQVLPAERYNNALAQSCYLVTAPELGKGEHRVYIAKQDDKPVAAVLEATAPDGYSG
                     AIQLLVGADFNGTVLGTRVTEHHETPGLGDKIELRLSDWLTHFAGKKISGADDAHWAV
                     KKDGGDFDQFTGATITPRAVVNAVKRAGLYAQTLPAQLSQLPACGE"
     gene            complement(7538..8596)
                     /gene="rsxD"
                     /locus_tag="OO357_003623"
     CDS             complement(7538..8596)
                     /gene="rsxD"
                     /locus_tag="OO357_003623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transport complex subunit RsxD"
                     /protein_id="HCR5888433.1"
                     /translation="MVFRIASSPYTHNQRQTSRIMLLVLLAAVPGIAAQLRFFGWGTL
                     VQILLASVSALLAEALVLKLRKQSVAATLKDNSALLTGLLLAVSIPPLAPWWMVVLGT
                     VFAVIIAKQLYGGLGQNPFNPAMIGYVVLLISFPVQMTSWLPPHEIAVNIPGFIDAIQ
                     VIFSGHTASGGDMNTLRLGIDGISQATPLDTFKTSVRAGHSVEQIMQYPIYSGILAGA
                     GWQWVNLAWLAGGVWLLWQKAIRWHIPLSFLVTLALCATLGWLFSPETLAAPQIHLLS
                     GATMLGAFFILTDPVTASTTNRGRLMFGALAGLLVWLIRSFGGYPDGVAFAVLLANIT
                     VPLIDYYTRPRVYGHRKG"
     gene            complement(8597..>8798)
                     /locus_tag="OO357_003624"
     CDS             complement(8597..>8798)
                     /locus_tag="OO357_003624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888434.1"
                     /translation="EPEEQVDPRKAAVEAAIARAKARKLEQQQANAEPEEQVDPRKAA
                     VAAAIARVQAKKAAQQKVVNED"
BASE COUNT         2236 a         2200 c         2246 g         2116 t
ORIGIN      
        1 catcaaattg aaaataatcc gcgcgttagc ctgctgttcc cgtggcatac ccttgaacgc
       61 caggtgatgg tgatcggtaa agcagaacga ctttcgactc tcgaagtgat gaaatatttt
      121 catagccgcc cgcgtgatag ccagattggt gcatgggttt cgaagcagtc cagtcgcatt
      181 tctgcccgcg gtatccttga aagtaaattc ctggagctga agcagaagtt tcaacagggc
      241 gaagtgccat tgccgagctt ttggggcggt tttcgcgtca gccttgaaca gattgagttc
      301 tggcagggcg gtgagcatcg cctgcatgac cgctttttgt accagcgtga aaatgatgcg
      361 tggaagattg atcgtcttgc accctgaaaa gatgcaaaaa tcttgcttta atcgctggta
      421 ctcctgattc tggcacttta ttctatgtct ctttcgcatc tggcgaaaag tcgtgtaccg
      481 gcaaaggtgc agtcgttata tacatggaga ttttgatggc aagcagtaac ttgattaaac
      541 aattgcaaga gcgggggctg gtagcccagg tgacggacga ggaagcgtta gcagagcgac
      601 tggcgcaagg cccgatcgcg ctctattgcg gcttcgatcc taccgctgac agcttgcatt
      661 tggggcatct tgttccattg ttatgcctga aacgcttcca gcaggcgggc cacaagccgg
      721 ttgcgctggt aggcggcgcg acgggtctga ttggcgaccc gagcttcaaa gctgccgagc
      781 gtaagctgaa caccgaagaa actgttcagg agtgggtgga caaaatccgt aagcaggttg
      841 ccccgttcct cgatttcgac tgtggagaaa actctgctat cgcggcgaac aactatgact
      901 ggttcggcaa tatgaatgtg ctgaccttcc tgcgcgatat tggcaaacac ttctccgtta
      961 accagatgat caacaaagaa gcggttaagc agcgtctcaa ccgtgaagat caggggattt
     1021 cgttcactga gttttcctac aacctgttgc agggttatga cttcgcctgt ctgaacaaac
     1081 agtacgatgt ggtgctgcaa attggtggtt ctgaccagtg gggtaacatc acttctggta
     1141 tcgacctgac ccgtcgtctg catcagaatc aggtgtttgg cctgaccgtt ccgctgatca
     1201 ccaaagcaga cggcaccaaa tttggtaaaa ctgaaggcgg cgcagtctgg ttggatccga
     1261 agaaaaccag cccgtacaaa ttctaccagt tctggatcaa cactgcggat gccgacgttt
     1321 accgcttcct gaagttcttc acctttatga gcattgaaga gatcaacgcc ctagaagaag
     1381 aagataaaaa cagcggtaaa gcaccgcgcg cccagtatgt actggcggag caggtgactc
     1441 gtctggttca cggtgaagaa ggtttacagg cggcaaaacg tattaccgaa tgcctgttca
     1501 gcggttcttt gagtgcgctg agtgaagcgg acttcgaaca gctggcgcag gacggcgtac
     1561 cgatggttga gatggaaaag ggcgcagacc tgatgcaggc gctggtcgat tctgaactgc
     1621 aaccttcccg tggtcaggcg cgtaaaacta tcgcctccaa tgccatcacc attaacggtg
     1681 aaaaacagtc cgatcctgaa tacttcttta aagaagaaga tcgtctgttt ggtcgtttta
     1741 ccttactgcg tcgcggcaaa aagaattact gtctgatttg ctggaaataa tgcattaagt
     1801 ggaaaggggg agtgagaaat cactccccct ggtttttata cagggaacat gatgaaaaat
     1861 attctcgcta tccagtctca cgttgtttat ggtcatgcgg gtaacagtgc ggcagagttt
     1921 ccgatgcgcc gcctgggcgc gaacgtctgg ccgctgaaca ccgttcaatt ttctaatcac
     1981 acccaatacg gcaaatggac tggctgcgtg atgccgccca gccatttaac cgaaattgtg
     2041 caaggcattg ccgccattga taaattacac acctgtgatg ccgtattaag tggctatctg
     2101 ggatcggcgg agcagggtga acatatcctc ggtatcgtcc gtcaggtgaa agcagcgaat
     2161 ccgcaggcga aatatttttg cgatccggta atgggtcatc cggaaaaagg ctgtatcgtt
     2221 gcaccgggtg tcgcagagtt tcatgtgcgg cacggtttgc ctgccagcga tatcattgcg
     2281 ccgaatctgg ttgagctgga aatactctgt gagcatgcgg taaataacgt cgaagaagcg
     2341 gttctggcag cgcgcgaact cattgagcaa gggccacaaa ttgtgttggt taaacacctg
     2401 gcgcgagctg gctacagccg tgaccgtttt gaaatgctgc tggtcaccgc cgatgaagtc
     2461 tggcatatca gccgtccact ggtggatttt ggtatgcgcc agccggtagg tgttggtgat
     2521 gtgacgagcg gtttactgct ggtgaaactg cttcaggggg caacgctgca ggaggccctg
     2581 gaacatgtga ccgctgcagt ctacgaaatc atggtgacca ccaaagcaat gcaggaatat
     2641 gagctgcaag tggtggctgc tcaggatcgt attgccaaac cagaacatta cttcagcgca
     2701 acaaagctct gaaatatctc atttcggccc acaacgacct gtgggccgat ggctattact
     2761 ttaagccttc cgctgacagc gcgtcttgta cttccggacg ttcagccata cgttgcataa
     2821 atgccgcaat gtgctctaac ccttccagat tcagtttcac cgcgtatgcc cagcgcagaa
     2881 ccgtaaacag ataggcatca gcaattgtaa atctttgccc gcagatccag tgctcatcct
     2941 tcagtgcctc gttcacatat tgcagcttct tatccagctg cgcgcgaact gtcggtttgt
     3001 actcttccgg tgtatctggg cgaaacagag gtgtgaaacc tttatgcagc tcggtggcga
     3061 tgtaattcag ccattcgatg gttttatagc gggaaatact gtttaccggt gccagcaact
     3121 ggcggtcggg gacgctgtcg gcaagatact gcataatcgc aacgccttcc gtcagcaaag
     3181 taccatcgtc cagcagcaat gcaggcacct gccccttagg gttaacggaa aagtaatcgt
     3241 caccgttttc gagacgtttt ttcattaaat ccacactgac gagcgtaaaa tcctttccgc
     3301 tctcacgcag ggtgatatgg gaagcgagag agcaggcgcc cggtttgtag aacaatttca
     3361 tcggtaactc cttttgtctg tggtttaaag tatggtagtg cgttgctgaa caaaaaaaaa
     3421 gccgctaacg aaaagttagc ggcttgtaaa agagtttccc tgaattttac gctacagtag
     3481 ctttcgccgc tttgtctgca gcgtcatcct gcgtcatgcg gtgcagttta ggcgcggtca
     3541 gcagcatcag tactgcaata acggcagtag cgacaccaat ctgcaagaat acgcgaccat
     3601 agacttccag tgacatcagc gggtcggtaa cgttatccgg cacagccatc atacccgcaa
     3661 cataaccacc aatcaggttt gcaccggcag tggtcaggaa ccagctaccc ataatgaagc
     3721 ccatcagacg ctgcggaacg agttgtgcaa ccattgccag acccagacca gagatcatca
     3781 gttccccgat gctctgcagg ccatagcttg cgaccagcca gctgacagac acgatacctg
     3841 cgtcagacgc gaatttcgca cccagcggca gaatcaggaa cgcaccagaa cacatcacca
     3901 tgccgattgc aaacttggtt ggcatcggca gggtatcacc catcttgtta tagatagcgg
     3961 ccagaatcgg actaccgatg atgatccaga acgggttcag tgcctgatac tgctcaggtt
     4021 ctacggccag acccagaatg gagtgctcaa cgttacgaat cgcaaagaag ttcagtgacg
     4081 ttggcatctg gctgtacagc acgaagaaga tgattgcttc gagcatcagg atgaaggcaa
     4141 cgatcatttt acgacgcgca gcacctttca tcgcgaaagc ttctttaccg aagataacca
     4201 caataccgaa ggcaacaacg cccagcgcca tacgcgcaac ttcctgattg tgcagtagcc
     4261 aggtggcaac agcgatcagc gccacaacac caataatggt cagcaacagg ttacggtagt
     4321 tgactggttc aaagtctggt tttgaaccgt actgtttaac ccagcgttgg cagaaggcga
     4381 agttaacgat agtgatcagc aggcctacaa cgctcaacgc aaacgcaaca ctccagccgt
     4441 atttcgcggc cagccacggg gtggctatca tagagaagaa agagccgatg ttgacggaca
     4501 tgtagtacat ggtgaatgca ccgtccagac gtgggtcgtt tttctcatag catgtagaaa
     4561 gcagagaaga cgggttagct ttaaacaggc cgttaccgac cgcaatagcc gccataccca
     4621 tataaacaat accggcgtcg tgaccagacc aggcaaccag agcataacca atcgccagca
     4681 caatagcgcc gagcataatt acgcgtttag tacccagtac cttgtcacct aaccagccgc
     4741 cgatagcgac cagaccataa accagggcac taaaggaaga gaaaagggtg attgaatccg
     4801 cttcagacat acccagttgt ttaaccaggt aaacagccat aattccttgt aggccgtaat
     4861 aaccaaaacg ttcccataac tcaatcgaga agatgagata gaacgccttc ggttgtttga
     4921 aagcgttcaa actgacgctt tcagttggtt tttggtttgc agtggacact tttacctctt
     4981 tttttacatc ccatattaac gggggtgctt tctcgcgtta tgtctaaaaa acatacgcgc
     5041 aattgttata gtgggagggg aaacggcagg taatgttcac tatcttgcac tttcagacaa
     5101 gtcctttgta ataatctgtt acatataact gaggcgggga attcaaccat tccgggaaat
     5161 gttatccagc gttaaatttt tcaaaaaacg taacggcaga ttttttcact gcattgagag
     5221 ggggatgatg gcttggcggc gatatgttct gctatttagc gtttttccta gtgagatagt
     5281 ccagtttctg aaaaatagcc agtgtaatgt tttgcaggtc aatatttgtt gcgtgtgtaa
     5341 atttaaatca ttttaacctg attcttgcaa tgtttttaca ttgccacagc atcgtgatct
     5401 tgatcacgaa tgaatagaaa atttcgctat taaaaaaaca attaacctgc atttttggtt
     5461 atccgcaaac ggattacctg atgataacgc taagcaatgg catcattgac gttaaaaaga
     5521 tgttttaaaa gaggataaag aaaggttatc aatggggtaa tcggtgttac cccttttctt
     5581 cagatgtcaa ctttctcttt gtattcacaa agatcttcaa taatacaaga gccacagcgg
     5641 ggcttgcggg caatgcaggt ataacgcccg tgcaggatca accaatggtg gcagtcgact
     5701 ttaaactctg ccggaaccac tttcagtagc ttttcttcta cctgttcgac gtttttcccc
     5761 ggcgcaaatt gagtacgatt acaaacgcgg aaaatgtgcg tgtcgacagc aatagtcggc
     5821 cagccgaatg cagtgtttaa tacgacgttg gctgttttac gacctacgcc gggcagggct
     5881 tcaagcgcag cacgatcttc cggaacctcg ccattatgtt gttccagtaa tatacggcag
     5941 gttttgatga tattttctgc tttgctgtta taaaggccaa tcgttttgat ataggttttc
     6001 accccttcaa cgcctagttc aagcatcgct gcaggcgtat tcgccaccgg gtagagtttc
     6061 gccgtcgcct tattaacact gatatcggtc gcctgagcgg aaagcagtac ggcaatcagc
     6121 aattcaaaag gcgaactgaa attaagctcg gtggtgggat gaggattgtt ctcacgcagg
     6181 cgagtgagga tctccagacg ttttgctttg ttcatcagac attccctgtt tcaccgtttg
     6241 gcaatgcacg ttcggcagct gcttcagcac ggcgcttttt cattctttca tcaatcaggt
     6301 attttcctgc cagcatcagt cccaagccaa taaatgcacc tggtggcagc atcgccagca
     6361 ggaaagggga gtcggtgtgg aaaatctcca cgcgtaatac ttttgcccag ctacctaaca
     6421 gcgcatctgc accgtcaaac aacgtgccat tgccgataat ttcgcgtagt gaacccagca
     6481 cgaacatggc gcaggttgcg cccgtaccaa ttgaaaagcc gtccagtgcc gaaagcgccg
     6541 gacctttttt ggcggcgaag gcttcagcgc ggcccacaac gatacagtta gtgacaatca
     6601 gcggaataaa aatccctaat gattgataca ggccaaaggc gtaggcgttg atcagcatct
     6661 gtacagcgct gaccaccgag gcgatgatca tcacgtaaat gggaatgcgg atctcggctg
     6721 gcgtccagtg acgcagcgtc gaaatggtca ggttggtcag cgtcagtacc agcgtagtcg
     6781 caagtcctaa acccagagcg ttagtggcag tggatgtgac cgccaatagc ggacaaaggc
     6841 cgagcagttg gaccagtgca gagttgtttt tccacaaccc ctgaacaata acgtctttaa
     6901 tttcgctcac gctttattct ccacaggcag gaagttgaga aagttgtgcc ggtaacgtct
     6961 gagcgtacaa tccggcgcgt tttaccgcat taaccaccgc gcggggagta atcgtcgcgc
     7021 cggtgaactg gtcgaaatca ccaccatctt tcttcaccgc ccagtgcgca tcatccgcac
     7081 cactgatttt ttttcccgca aaatgggtga gccagtcaga aaggcgcaat tcgattttat
     7141 cgccaagccc tggcgtttcg tggtgctctg tcacgcgcgt gccaagtacc gtgccattaa
     7201 aatcggctcc caccagcagc tgaatcgcac cggaatagcc atctggcgcg gttgcttcca
     7261 gaacggcggc taccggtttg tcatcctgtt tggcgatgta aacccgatgc tcacctttac
     7321 ctaactctgg cgcagttacc agatagcaac tctgtgcgag cgcattgtta tagcgttcgg
     7381 ctggcagcac ctgatcaaat aacgtctttt gttgcagact ggcctgttca gcaattgtcg
     7441 ttttggtcat ctggttgatg gccgcagtta accctgtaga acccgctgca aatagtgcca
     7501 gtgtaatgcc gtgttttcgg atagttttca gcatggttta ccctttgcga tggccgtaga
     7561 cgcgcggacg cgtgtagtaa tcgatcagag gaaccgtgat gttcgccagc aggacggcaa
     7621 aagccacgcc gtcaggatag ccgccgaaac tgcggatcaa ccagactaat aagcccgcca
     7681 gcgcgccgaa catcagccga ccacgattgg tcgtagaagc agtaactggg tcagtcaaaa
     7741 taaagaatgc gccgagcata gtcgcaccag acagcagatg aatttgcggt gctgccagtg
     7801 tttctggtga gaacaaccag cccaacgttg cgcataacgc cagcgttact aagaagctga
     7861 ggggaatatg ccagcgaatc gctttctgcc atagcaacca cacgccacca gccagccagg
     7921 cgagatttac ccattgccaa ccagcgcccg ccagaatacc gctgtagatc ggatattgca
     7981 taatctgttc aaccgaatga cctgcgcgga cagaggtttt gaatgtatcc agcggtgtcg
     8041 cctgactaat gccatcaata cctaagcgta gcgtattcat atcaccacca ctggcggtat
     8101 gaccgctgaa aataacctgt atggcgtcga taaaaccagg gatgttgacc gcaatttcat
     8161 gtggcggtaa ccagctggtc atctgcaccg ggaaggagat cagtaagacc acataaccaa
     8221 tcattgccgg attaaacggg ttttgcccca gaccgccata caactgttta gcgatgatca
     8281 ccgcaaacac cgtacccagt acgaccatcc accatggcgc taggggggga atacttaccg
     8341 ccagcaataa gcctgtcagc aatgctgagt tatctttcaa cgttgcggct accgactgct
     8401 tgcgtagttt gagtacgaga gcttcggcta acagagcact gaccgacgcc aacaggatct
     8461 gaacgagagt accccaacca aaaaaccgca gttgcgctgc gattcctggc acggctgcga
     8521 gcaacaccaa cagcataatg cgcgatgtct ggcgctggtt atgggtataa ggggagctag
     8581 ctattctgaa taccatttag tcctcgttta caaccttttg ctgggcggct tttttggcct
     8641 gaacgcgggc aatagccgcg gcaactgcgg ctttgcgcgg atcgacctgt tcttctggtt
     8701 ccgcattagc ctgttgctgt tccagcttgc gtgctttggc gcgggcaata gcggcttcga
     8761 cggcagcttt gcgcggatca acctgttctt ctggctcc
//
LOCUS       DAJTRC010000140         8675 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.140, whole genome shotgun sequence.
ACCESSION   DAJTRC010000140 DAJTRC010000000
VERSION     DAJTRC010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8675)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8675)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8675
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.140"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(803..1999)
                     /gene="hemY"
                     /locus_tag="OO357_003625"
     CDS             complement(803..1999)
                     /gene="hemY"
                     /locus_tag="OO357_003625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX biogenesis protein HemY"
                     /protein_id="HCR5888435.1"
                     /translation="MLKVLLLFVLLIAGIVVGPMIAGHQGYVLIQTDNYNIETSVTGL
                     AIILILAMVVLFAIEWLLRRIFRTGAHTRGWFVGRKRRRARKQTEQALLKLAEGDYQQ
                     VEKLMAKNADHAEQPVVNYLLAAEAAQQRGDEARANQHLERAAELAGNDTIPVEITRV
                     RLQLARNENHAARHGVDKLLEVTPRHPEVLRLAEQAYIRTGAWSSLLDIIPSMAKAHV
                     GDEEHRAMLEQQAWIGLMDQARADNGSEGLRNWWKNQSRKTRHQVALQVAMAEHLIEC
                     DDHDTAQQIIIDGLKRQYDDRLLLPIPRLKTNNPEQLEKVLRQQIKNVGDRPLLWSTL
                     GQSLMKHGEWQEASLAFRAALKQRPDAYDYAWLADALDRLHKPEEAAAMRRDGLMLTL
                     QNNPPQ"
     gene            complement(2002..3213)
                     /gene="hemX"
                     /locus_tag="OO357_003626"
     CDS             complement(2002..3213)
                     /gene="hemX"
                     /locus_tag="OO357_003626"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HCR5888436.1"
                     /translation="MTEQEKTSAVVEETREAVDTTSQPVATEKKSKNNTALILSAVAI
                     AIALAAGVGLYGWGKQQAVNQTATSDALANQLTALQKAQESQKAELEGIIKQQAAQLK
                     QANRQQETLAKQLDEVQQKIATISGSDAKTWLLAQADFLVKLAGRKLWSDQDVTTSAA
                     LLKSADASLADMNDPSLITVRRAITDDIASLSAVSQVDYDGIILKLNQLSNQVDNLRL
                     ADNDSDGSPMDSDGEELSSSISEWRINLQKSWQNFMDNFITIRRRDDTAVPLLAPNQD
                     IYLRENIRSRLLVAAQAVPRHQEETYRQALENVSTWVRAYYDTDDATTKAFLDEVDQL
                     SQQNISMDLPETLQSQAMLEKLMQTRVRNLLAQPAAGTTEAKPAPAPAPAPAPAPAPQ
                     GDTPAAAPQGE"
     gene            complement(3235..3975)
                     /gene="hemD"
                     /locus_tag="OO357_003627"
     CDS             complement(3235..3975)
                     /gene="hemD"
                     /locus_tag="OO357_003627"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HCR5888437.1"
                     /translation="MSILVTRPSPAGEELVSRLRTLGQVAWHFPLIEFSPGRQLPQLA
                     DQLAALGESDLLFALSQHAVAFAQSQLHQQDRKWPQLPTYFAIGRTTALALHTVSGQK
                     ILYPQDREISEVLLQLPELQNIAGKRALILRGNGGRELIGDTLTVRGAEVTFCECYQR
                     CAIHYDGAEEAMRWQSREVTMVVVTSGEMLQQLWSLIPQWYREHWLLHCRLLVVSERL
                     AKLARELGWQDIKVADNADNDALLRALQ"
     gene            complement(3972..4913)
                     /gene="hemC"
                     /locus_tag="OO357_003628"
     CDS             complement(3972..4913)
                     /gene="hemC"
                     /locus_tag="OO357_003628"
                     /EC_number="2.5.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylbilane synthase"
                     /protein_id="HCR5888438.1"
                     /translation="MLDNVLRIATRQSPLALWQAHYVKDKLMASHPGLVVELVPMVTR
                     GDVILDTPLAKVGGKGLFVKELEVALLENRADIAVHSMKDVPVEFPQGLGLVTICERE
                     DPRDAFVSNNYDSLDALPAGSIVGTSSLRRQCQLAERRPDLIIRSLRGNVGTRLSKLD
                     NGEYDAIILAVAGLKRLGLESRIRAALPPEISLPAVGQGAVGIECRLDDTRTRELLAA
                     LNHHETALRVTAERAMNTRLEGGCQVPIGSYAELIDGEIWLCALVGAPDGSQIIRGER
                     RGAPQDAEQMGISLAEELLNNGAREILAEVYNGEAPA"
     gene            5300..7846
                     /gene="cyaA"
                     /locus_tag="OO357_003629"
     CDS             5300..7846
                     /gene="cyaA"
                     /locus_tag="OO357_003629"
                     /EC_number="4.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate cyclase"
                     /protein_id="HCR5888439.1"
                     /translation="MYLYIETLKQRLDAINQLRVDRALAAMGPAFQQVYSLLPTLLHY
                     HHPRMPGYLDGNVPKGICLYTPDETQRHYLNELELYRGMSVQDPPKGELPITGVYTMG
                     STSSVGQSCSSDLDIWVCHQSWLDSEERQLLQRKCSLLESWAASLGVEVSFFLIDENR
                     FRHNESGSLGGEDCGSTQHILLLDEFYRTAVRLAGKRILWNMVPCDEEEHYDDYVMTL
                     YAQGVLTPNEWLDLGGLSSLSAEEYFGASLWQLYKSIDSPYKAVLKTLLLEAYSWEYP
                     NPRLLAKDIKQRLHDGEIVSFGLDPYCMMLERVTEYLTAIEDFTRLDLVRRCFYLKVC
                     EKLSRERACVGWRRAVLSQLVSEWGWDEARLAMLDNRANWKIDQVREAHNELLDAMMQ
                     SYRNLIRFARRNNLSVSASPQDIGVLTRKLYAAFEALPGKVTLVNPQISPDLSEPNLT
                     FIYVPPGRANRSGWYLYNRAPNIESIISHQPLEYNRYLNKLVAWAWFNGLLTSRTRLY
                     IKGNGIVDLPKLQEMVADVSHHFPLRLPAPTPKALYSPCEIRHLAIIVNLEYDPTAAF
                     RNQVVHFDFRKLDVFSFGENQNCLVGSVDLLYRNSWNEVRTLHFNGEQSMIEALKTIL
                     GKMHQDAAPPDSVEVFCYSQHLRGLIRTRVQQLVSECIELRLSSTRQETGRFKALRVS
                     GQTWGLFFERLNVSVQKLENAIEFYGAISHNKLHGLSVQVETNHVKLPAVVDGFASEG
                     IIQFFFEETQDENGFNIYILDESNRVEVYHHCEGSKEELVRDVSRFYSSSHDRFTYGS
                     SFINFNLPQFYQIVKVDGREQVIPFRTKSIGNLPPANQDHDTPLLQQYFS"
     gene            complement(7886..8206)
                     /gene="cyaY"
                     /locus_tag="OO357_003630"
     CDS             complement(7886..8206)
                     /gene="cyaY"
                     /locus_tag="OO357_003630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000999950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron donor protein CyaY"
                     /protein_id="HCR5888440.1"
                     /translation="MNDSEFHRLADQLWLTIEERLDDRDGDSDIDCEINGGVLTITFE
                     NGSKIIINRQEPLHQVWLATKQGGYHFDLKGDEWICDRSGETFWDLLEQAATQQAGET
                     VSFR"
     gene            complement(8254..>8675)
                     /locus_tag="OO357_003631"
     CDS             complement(8254..>8675)
                     /locus_tag="OO357_003631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5888441.1"
                     /translation="FHLITGNIAQYKDRLYVTKLSTFGEDQLEIIPLDTPDLVIDRSF
                     NSGKQHAYFIRQLRSKSLQIIPVNGSLTKNDRFAYDDRNVYTWTDTEVRITPSPCPAK
                     TRVREENVREVQNRDIIIPVTDESCRNAAAEVQTLKP"
BASE COUNT         1972 a         2241 c         2236 g         2226 t
ORIGIN      
        1 ctggttgtct ggagattcag ggggccagtc tagcagttga cggggtatct gctgcgctgg
       61 ccatgagagg aagagcggcg gcgacagcaa caacaagacg tttgggtgaa aacgaaaatt
      121 ccatgcaaaa tgctccggtt tcatgtcatc aaaatgatga cgtaattaag cattgataat
      181 tgagatccct ctccctgaca ggatgattac ataaataata gtgacaaaaa taaattattt
      241 atttatccag aaaatgaatt ggaaaatcag gagagcgttt tcaatcctac ctctggcgca
      301 gttgatatgt aaggcaggtt tattatatcg cgttgattat tgatgctgtt tttagtttta
      361 acggcaatta atatatgtgt tattaattga atgaatttta tcattcataa taagtatgtg
      421 taggatcaag ctcaggttaa atattcactc aggaagttat tactcaggaa gcaaagagga
      481 ttacagaatt atctcataac aagtgttaag ggatgttatt tcccgattct ctgtggcata
      541 ataaacgagt agatgctcat tccatctctt atgttcgcct tagtgcctca taaactccgg
      601 aatgacgcag agccgtttac ggtgcttatc gtccactgac agatgtcgct tatgcctcat
      661 cagacaccat ggacacaacg ttgagtgaag cacccacttg ttgtcataca gacctgtttt
      721 aacgcctgct ccgtaataag agcaggcgtt tttttatgca tcagaaaggt cccggaggtg
      781 ctttcctccg ggtgagaagg aactactgtg gcgggttatt ctgcaacgtt aacatcaaac
      841 cgtcgcgacg catcgctgca gcttcttccg gcttgtgcag tctgtccagc gcgtcggcaa
      901 gccatgcgta atcgtaggcg tccggacgtt gtttcagcgc cgcgcggaag gcgagcgatg
      961 cttcctgcca ttcaccgtgc ttcatcagtg actgacccaa tgtgctccac aacagcgggc
     1021 gatcaccgac gtttttgatt tgctggcgta gcactttttc cagctgttcc gggttattgg
     1081 ttttcagacg cgggatcggc agcagtaggc gatcgtcata ctggcgtttc aggccgtcga
     1141 tgataatttg ctgggcagta tcatgatcgt cacattcaat aagatgttcc gccattgcca
     1201 cctgcaacgc tacctgatga cgcgttttcc ggctttggtt tttccaccag ttacgcaaac
     1261 cttcactacc gttatcggca cgcgcctgat ccatcaggcc aatccatgcc tgttgttcca
     1321 gcattgcacg atgttcttca tcaccaacat gggctttcgc cattgatggg atgatatcca
     1381 gcagagaact ccatgcccct gtgcggatat acgcctgttc cgccagacgt aatacttcag
     1441 gatggcgtgg cgtaacttcc agcagcttat ccacgccgtg gcgtgcagca tggttttcat
     1501 tacgggccag ttgcagacgt acacgggtga tttctaccgg aatggtgtca ttgccggcca
     1561 gctccgctgc gcgttccaga tgttggttgg cgcgtgcttc atcaccacgt tgttgcgcgg
     1621 cttcggcagc cagtagatag ttcaccaccg gttgttcagc gtgatcggca tttttcgcca
     1681 tcagcttttc aacttgctga taatcacctt ccgccagttt cagcagcgcc tgttcggtct
     1741 gcttacgcgc acggcgacgc ttacgtccga caaaccatcc acgggtgtgc gcgccagtgc
     1801 ggaagatccg ccgcagtagc cactcaatgg caaacagcac taccatcgcc agaatcaata
     1861 tgatcgccag gcccgtgacg ctggtttcga tattgtagtt gtcggtctgg atcagcacat
     1921 aaccctgatg accggcaatc atcgggccaa ccactatccc cgcaatcagc aaaacaaaga
     1981 gtaataacac ttttagcatg gttattctcc ttgcggcgcg gctgccggag tatcaccttg
     2041 cggtgcaggt gcaggtgcag gtgcaggtgc aggtgcaggt gcaggtttag cttccgttgt
     2101 ccccgctgcc ggttgtgcca gcaggttacg cacgcgagtc tgcatcagtt tttccagcat
     2161 cgcctggctt tgcagggttt ccggaagatc catcgagata ttttgctggc ttaactggtc
     2221 cacctcgtcg aggaacgctt tggtggtggc atcatcagta tcgtagtaag cacgtaccca
     2281 ggtggagacg ttctccagcg cctggcgata agtctcttcc tgatgacgcg gtacagcttg
     2341 tgctgcgacc agcaggcgag agcgaatatt ttcgcgcaga tagatatcct gatttggcgc
     2401 taacagcggt acggcggtgt catcacgacg gcgaatcgta atgaagttgt ccataaagtt
     2461 ctgccagctt ttttgcagat tgatacgcca ttcgctgatg gaactggaaa gctcttcacc
     2521 gtctgaatcc atcggcgaac catcgctgtc attatcggcc agacgcaggt tatctacctg
     2581 atttgaaagc tgattaagct taaggatgat gccgtcataa tccacctgcg atactgcaga
     2641 aaggctggcg atatcatcgg taattgcccg acgaacggta atcagactcg ggtcattcat
     2701 atccgccagg ctggcgtctg cacttttcag caacgctgca gaggtcgtga cgtcctgatc
     2761 gctccacagc ttccgtccgg cgagtttcac cagaaaatcg gcctgagcca gcagccaggt
     2821 tttagcatcg ctgccggaaa tggtggcgat cttttgttgg acttcatcca actgttttgc
     2881 cagcgtttct tgctgacgat tcgcctgctt aagctgcgca gcttgttgct taataatgcc
     2941 ttccagctcg gctttttggc tctcctgggc tttttgcaat gccgtcagtt ggttagccag
     3001 ggcatcgctg gtggcggtct gattgacggc ctgttgttta ccccagccat acaaaccgac
     3061 acccgccgcc agagcaatag cgatagccac cgcgctgaga atcaatgcag tattgttctt
     3121 actctttttt tctgttgcga caggttgtga cgtggtgtcc acggcctccc tggtctcttc
     3181 aaccacggcg gaggtttttt cttgttccgt cattatggct tcctgttatg agagttattg
     3241 taatgcccgt aaaagcgcat cgttgtcagc gttatcggcg accttaatgt cttgccagcc
     3301 cagttcccgg gcgagtttcg ccaaacgctc actgacgacc aatagtcgac agtgtagtaa
     3361 ccagtgctca cgataccatt gtgggatcag cgaccagagt tgctgcaaca tttcaccgct
     3421 ggtaacaacg accatcgtca cctcgcggga ttgccagcgc atcgcttctt ctgcaccatc
     3481 gtaatggatt gcgcatcgtt gataacattc acaaaaagtg acctcagcac cgcgcaccgt
     3541 cagggtatcc ccaattagct cacgaccgcc attgccacgt aatatcagcg cacgtttgcc
     3601 cgcaatattt tgtaattcag gtaattgtag caagacttcg ctgatttccc gatcctgtgg
     3661 gtagagaatc ttctgtccgc ttacggtatg tagcgccagt gcggtggtgc gtccaatggc
     3721 gaaataagta ggtagttggg gccatttacg atcttgctga tgcagctgtg attgggcaaa
     3781 agcaaccgcg tgttgcgaga gggcaaacaa cagatcgctc tcccccagcg ctgccagttg
     3841 atcagcaagt tgcggtaatt gtcgacccgg agaaaactca atcagcggaa aatgccaggc
     3901 cacctgcccc agtgtgcgca gacggctcac taactcttct ccagcgggag acgggcgggt
     3961 gaccaggata ctcatgccgg ggcctctccg ttatagactt cagcgaggat ctcgcgcgcg
     4021 ccgttattca gtagctcttc tgccagcgaa atccccattt gttcggcatc ttgcggcgca
     4081 ccgcggcgtt caccgcgaat aatctgcgaa ccgtccggcg cgccgaccag cgcacatagc
     4141 cagatttcgc catcaataag ctcggcgtag ctaccaattg gcacctgaca tccgccttcg
     4201 agacgggtat tcatggcgcg ttctgcggta acgcgcagtg cagtttcgtg gtgattcagc
     4261 gcggcaagca gctcgcgagt gcgtgtatca tcaaggcggc attcaatacc gactgcacct
     4321 tgtcctaccg ccggaagaga aatctcgggt ggtaacgcgg cgcgaatgcg ggactccaga
     4381 cctaaacgtt ttagtccggc tacggcaaga atgatggcat cgtattcgcc gttatctagt
     4441 ttgctcaggc gagtgccgac gttgccgcgc agggagcgga taatcagatc cgggcggcgt
     4501 tcagccagtt ggcactggcg acgtaaactg gacgtcccga cgatactgcc tgctggtaac
     4561 gcatccagac tgtcatagtt attggacaca aaggcatcgc gaggatcttc acgctcgcaa
     4621 atagtgacca gccccagacc ttgcgggaat tcaaccggca catctttcat tgaatgtacg
     4681 gcgatatcag cgcgattctc caggagcgcg acttccagct cttttacaaa taagcctttt
     4741 ccgcctactt tcgccagcgg cgtatcaaga atcacatcgc cgcgcgtcac catcggtacc
     4801 agttcaacga ccaggcccgg atggctcgcc atcaacttgt ctttgacata gtgtgcctgc
     4861 cagagtgcaa gtgggctttg gcgtgtggca attcttaaaa cattgtctaa catgcttgtt
     4921 accgtcatta tcatccgtgg tccatcctaa catccttgcc agagtgatgt cagtgttgtg
     4981 gtgaaacgta gacgcctgcg caaaccgtaa aatgaggtct ggcagtggat cctgacaggc
     5041 gtttcacgcc gtcgtaataa ggaatttaca gagaataaac ggtgctacac ttgtatgtag
     5101 cgcatctttc tttacggtca atcagcaagg tgttaaattg atcacgtttt agaccatttt
     5161 ttcgtcgtga aactaaaaaa accaggcgcg aaaagtggta acggttacct ttgacatacg
     5221 aaatatcccg aatgccgcgt gttaccgttg atgttggcgg aatcacagtc atgacgggta
     5281 gcaaatcagg cgatacgtct tgtacctcta tattgagact ctgaaacaga gactggatgc
     5341 cataaatcaa ttgcgtgtgg atcgcgcgct tgctgctatg gggcctgcat tccaacaggt
     5401 ctacagtcta ctgccgacat tgttgcacta tcaccatccg cgaatgccgg gttaccttga
     5461 tggtaacgtt cccaaaggca tttgccttta cacgcctgat gaaactcaac gccactacct
     5521 gaacgagctt gaactgtatc gtggaatgtc agtacaggat ccaccgaaag gtgagcttcc
     5581 aattactggt gtatacacca tgggcagcac ctcgtccgta gggcaaagtt gttcctctga
     5641 cctggatatc tgggtctgtc atcaatcctg gctcgatagc gaagagcgcc agttactaca
     5701 acgtaaatgc agcctgctgg aaagctgggc cgcctcgctg ggtgtggaag tcagcttctt
     5761 cctgattgat gaaaaccgct tccgtcataa tgaaagcggc agcctggggg gcgaagattg
     5821 tggctccacc cagcatatac tgctgcttga cgaattttat cgtactgccg tgcgtctcgc
     5881 cggtaagcgt attctgtgga atatggtgcc gtgcgacgaa gaagagcatt acgacgacta
     5941 tgtgatgacg ctttacgcgc agggcgtgct gacgccaaat gaatggctgg atctcggtgg
     6001 cttaagctcg ctttctgctg aagagtactt tggtgccagc ctttggcagc tctacaagag
     6061 tatcgattcc ccatacaaag cggtgctgaa aacactgctg ctggaagcct attcctggga
     6121 atacccgaac ccacgtctgc tggcgaaaga tatcaaacag cgtttgcacg acggcgagat
     6181 tgtatcgttt ggcctcgatc catactgcat gatgctggag cgtgttactg aatacctgac
     6241 ggcgattgaa gatttcaccc gtctggattt agtacgtcgc tgcttctatt taaaagtgtg
     6301 cgaaaaactc agccgtgaac gcgcctgtgt aggctggcgt cgcgcagtgt tgagccagtt
     6361 agtgagcgag tggggttggg acgaagctcg tctggcaatg ctcgataacc gtgctaactg
     6421 gaagattgat caggtgcgtg aggcgcacaa cgagctgctc gacgcgatga tgcagagcta
     6481 ccgtaatctg atccgctttg cgcgtcgcaa taaccttagc gtctccgcca gtccgcagga
     6541 tatcggcgta ctgacgcgta aactgtatgc cgcgtttgaa gcattgccag gtaaagtgac
     6601 gctggtaaac ccgcagattt cacccgatct ctcggaaccg aatctgacct ttatttatgt
     6661 gccgccgggc cgggctaacc gttcaggttg gtatctgtat aaccgcgcgc caaatattga
     6721 gtcgatcatc agccatcagc cgctggaata taaccgttac ctgaataaac tggtggcgtg
     6781 ggcatggttt aacggcctgc tgacctcgcg cacccgtttg tatattaaag gtaacggcat
     6841 tgtcgatttg cctaagttgc aggagatggt cgccgacgtg tcgcaccatt tcccgctgcg
     6901 cttacctgca ccgacaccga aggcgctcta cagcccgtgt gagattcgcc atctggcgat
     6961 tatcgttaac ctggaatatg acccgacagc tgcgttccgc aatcaggtgg tgcattttga
     7021 tttccgtaag ctggatgtct tcagctttgg cgagaatcaa aattgcctgg taggtagcgt
     7081 tgacctgctg taccgcaact cgtggaacga agtgcgtacg ctgcacttca acggcgagca
     7141 atcgatgatc gaagccctga aaactattct cggcaaaatg catcaggacg ccgcaccgcc
     7201 agatagcgtg gaagtcttct gttatagcca gcatctgcgc ggcttaatcc gtactcgcgt
     7261 gcagcaactg gtttctgagt gtattgaact gcgcctttcc agcacccgcc aggaaaccgg
     7321 gcgtttcaag gcgctgcgcg tttctggtca aacctggggc ttgttcttcg aacgcctgaa
     7381 tgtatcggta cagaagctgg agaacgccat cgagttttat ggcgctattt cgcataacaa
     7441 actgcacggc ttgtcagtgc aggttgaaac caatcacgtc aaattaccgg cggtggtgga
     7501 cggctttgcc agcgaaggga tcatccagtt ctttttcgaa gaaacgcaag acgagaatgg
     7561 ctttaatatc tacattctcg acgaaagcaa ccgggttgag gtgtatcacc actgcgaagg
     7621 cagcaaagag gagctggtac gtgacgtcag tcgcttctac tcgtcatcgc acgaccgctt
     7681 cacctacggc tcaagcttca tcaacttcaa cctgccgcag ttctatcaga ttgtgaaggt
     7741 tgatggtcgc gaacaggtga ttccgttccg aacaaaatcg attggcaacc tgccgcccgc
     7801 caatcaggat cacgatacgc cgctattaca gcagtatttt tcgtgatgag cgtgccggaa
     7861 agcgaggctt atccggcatg caatcttagc ggaaactgac tgtttcaccc gcctgttgcg
     7921 tcgccgcctg ttccagcaaa tcccagaagg tttcgccgct gcgatcgcaa atccattcat
     7981 cgcctttcag gtcaaaatgg tagccgccct gtttggttgc cagccacacc tggtgcagcg
     8041 gctcctggcg gttaataatg attttgctgc cgttttcaaa ggtaatggtc agtacgccgc
     8101 cgttgatttc gcagtcgata tcgctgtcgc catccctgtc gtccagacgc tcttcaatgg
     8161 tcagccacag ttgatcagcc aggcgatgaa attcactgtc gttcattgtt gtatcctgtt
     8221 tttaagtgat ggcggcagta tagcggcatg gggtcagggc ttcaaagttt gcacctctgc
     8281 tgctgcgttc cggcatgatt catccgtcac cggaataatg atgtctctgt tttgaacttc
     8341 acgtacgttc tcctctctga cacgagtttt cgccgggcag ggggagggcg taatccttac
     8401 ctctgtatcg gtccatgtat aaacattgcg atcgtcataa gcgaagcgat cgtttttagt
     8461 tagcgaaccg ttaactggaa taatttgcaa gctctttgac cgtaattggc ggataaagta
     8521 agcatgctgt ttgccgctat taaatgagcg atcaatgacc aggtctggcg tatccagcgg
     8581 gattatctca agctggtctt caccaaatgt cgataatttt gttacataca gccgatcttt
     8641 atattgcgca atattgccag taatcagatg gaatt
//
LOCUS       DAJTRC010000141         8412 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.141, whole genome shotgun sequence.
ACCESSION   DAJTRC010000141 DAJTRC010000000
VERSION     DAJTRC010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8412
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.141"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..155
                     /gene="csgC"
                     /locus_tag="OO357_003632"
     CDS             <1..155
                     /gene="csgC"
                     /locus_tag="OO357_003632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706954.2"
                     /note="involved in autoagglutination of curliated cells;
                     not involved in production of curli fibers; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="curli assembly protein CsgC"
                     /protein_id="HCR5888442.1"
                     /translation="TLSLPANQPIALTKLSLNISPEDRVKIVVTVSDGQALHLSQQWT
                     PSSEKS"
     gene            276..587
                     /locus_tag="OO357_003633"
     CDS             276..587
                     /locus_tag="OO357_003633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5888443.1"
                     /translation="MFRPFLDSLMLGSLFFPFIAIAGSTAQGGVIHFYGQIVEPACDV
                     STQSSPVEMNCPQNGSIPGKTYSSKALMSGNVKNAQIASVKVQYLDKQKKLAVMNIEY
                     N"
     gene            682..1215
                     /gene="ymdB"
                     /locus_tag="OO357_003634"
     CDS             682..1215
                     /gene="ymdB"
                     /locus_tag="OO357_003634"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HCR5888444.1"
                     /translation="MKTRIHVVQGDITKLAVDVIVNAANPSLMGGGGVDGAIHRAAGP
                     ALLDACLKVRQQQGDCPTGHAVITLAGDLPAKAVVHTVGPVWRGGEQNEDQLLQDAYL
                     NSLRLVAANSYTSVAFPAISTGVYSYPRAAAAEIAVKTVSEFITRHALPEQVYFVCYD
                     EENAHLYERLLTQQGDE"
     gene            1157..2638
                     /gene="clsC"
                     /locus_tag="OO357_003635"
     CDS             1157..2638
                     /gene="clsC"
                     /locus_tag="OO357_003635"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase ClsC"
                     /protein_id="HCR5888445.1"
                     /translation="MMKKTPTSTKDSLPNKEMNDLPRLASAVLPLCSQHPGQCGLFPL
                     EKSLDAFAARYRLAEMAEHTLDVQYYIWQDDMSGRLLFSALLAAAKRGVRVRLLLDDN
                     NTPGLDDILRLLDSHPRIEVRLFNPFSFRLLRPLGYITDFSRLNRRMHNKSFTVDGVV
                     TLVGGRNIGDAYFGAGEEPLFSDLDVMAIGPVVEDVADDFARYWYCKSVSPLQQVLDV
                     PEGEMADRIELPASWHNDAMTHRYLRKMESSPFINHLVDGTLPLIWAKTRLLSDDPAK
                     GEGKAKRHSLLPQRLFDIMGSPSERIDIISSYFVPTRAGVAQLLRMVRKGVKIAILTN
                     SLAANDVAVVHAGYARWRKKLLRYGVELYELKPTREQSSTLHDRGITGNSGASLHAKT
                     FSIDGKTVFIGSFNFDPRSTLLNTEMGFVIESETLAQLIDKRFIQSQYDAAWQLRLDR
                     WGRINWVDRHAKKEVILKKEPATSFWKRVMVRLASILPVEWLL"
     gene            complement(2646..3803)
                     /gene="mdoC"
                     /locus_tag="OO357_003636"
     CDS             complement(2646..3803)
                     /gene="mdoC"
                     /locus_tag="OO357_003636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoC"
                     /protein_id="HCR5888446.1"
                     /translation="MNPVPAQREYFLDSIRAWLMLLGIPFHISLIYSSHTWHVNSAEP
                     SLWLTLFNDFIHSFRMQVFFVISGYFSYMLFLRYPLKKWWKVRVERVGIPMLTAIPLL
                     TLPQFIMLQYVKGKAESWPGLSLYDKYNTLAWELISHLWFLLVLVVMTTLCVWIFKRI
                     RNNLENSDKTNKKFSMVKLSVIFLCLGIGYAVIRRTIFIVYPPILSNGMFNFIVMQTL
                     FYLPFFILGALAFIFPHLKALFTTPSRGCTLAAALAFVAYLLNQRYGSGDAWMYETES
                     VITMVLGLWMVNVVFSFGHRLLNFQSARVTYFVNASLFIYLVHHPLTLFFGAYITPHI
                     TSNWLGFLCGLIFVVGIAIILYEIHLRIPLLKFLFSGKPVVKRENDKAPAR"
     gene            4197..5732
                     /gene="mdoG"
                     /locus_tag="OO357_003637"
     CDS             4197..5732
                     /gene="mdoG"
                     /locus_tag="OO357_003637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis protein MdoG"
                     /protein_id="HCR5888447.1"
                     /translation="MMKMRWLSAAVMLTLYTSSSWAFSIDDVAKQAQSLAGKGYEAPK
                     SNLPSVFRDMKYADYQQIQFNHDKAYWNNLKTPFKLEFYHQGMYFDTPVKINEVTATA
                     VKRIKYSPDYFTFGDVQHDKDTVKDLGFAGFKVLYPINSKDKNDEIVSMLGASYFRVI
                     GAGQVYGLSARGLAIDTALPSGEEFPRFKEFWIERPKPTDKRLTIYALLDSPRATGAY
                     KFVVMPGRDTVVDVQSKIYLRDKVGKLGVAPLTSMFLFGPNQPLPANNYRPELHDSNG
                     LSIHAGNGEWIWRPLNNPKHLAVSSFSMENPRGFGLLQRGRDFSRFEDLDDRYDLRPS
                     AWVTPKGEWGKGSVELVEIPTNDETNDNIVAYWTPDQLPEPGKEMNFKYTITFSRDED
                     KLHAPDNAWVQQTRRSTGDVKQSNLIRQPDGTIAFVVDFTGAEMKKLPEDTPVTAQTS
                     IGDNGEIVESTVRYNPVTKGWRLVMRVKVKDAKKITEMRAALVNADQTLSETWSYQLP
                     ANE"
     gene            5725..8268
                     /gene="mdoH"
                     /locus_tag="OO357_003638"
     CDS             5725..8268
                     /gene="mdoH"
                     /locus_tag="OO357_003638"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucans biosynthesis glucosyltransferase MdoH"
                     /protein_id="HCR5888448.1"
                     /translation="MNKTTEYIDAMPIAASEKAALPKTDIRAVHQALDAEHRTWARED
                     DSPQGSVKARLEQAWPDSLADGQLIKDDEGRDQLKAMPEAKRSSMFPDPWRTNPVGRF
                     WDRLRGRDVTPRYLARLTKEEQESEQKWRTVGTIRRYILLILTLAQTVVATWYMKTIL
                     PYQGWALINPMDMVGQDLWVSFMQLLPYMLQTGILILFAVLFCWVSAGFWTALMGFLQ
                     LLIGRDKYSISASTVGDEPLNPEHRTALIMPICNEDVNRVFAGLRATWESVKATGNAK
                     HFDVYILSDSYNPDICVAEQKAWMELIAEVGGEGQIFYRRRRRRVKRKSGNIDDFCRR
                     WGSQYSYMVVLDADSVMTGDCLCGLVRLMEANPNAGIIQSSPKASGMDTLYARCQQFA
                     TRVYGPLFTAGLHFWQLGESHYWGHNAIIRVKPFIEHCALAPLPGEGSFAGSILSHDF
                     VEAALMRRAGWGVWIAYDLPGSYEELPPNLLDELKRDRRWCHGNLMNFRLFLVKGMHP
                     VHRAVFLTGVMSYLSAPLWFMFLALSTALQVVHALTEPQYFLQPRQLFPVWPQWRPEL
                     AIALFASTMVLLFLPKLLSILLIWCKGTKEYGGFWRVTLSLLLEVLFSVLLAPVRMLF
                     HTVFVVSAFLGWEVVWNSPQRDDDSTSWGEAFKRHGSQLLLGLVWAVGMAWLDLRFLF
                     WLAPIVFSLILSPFVSVISSRATVGLRTKRWKLFLIPEEYSPPQVLVDTDRFLEMNRQ
                     RSLDDGFMHAVFNPSFNALATAMATARHRASKVLEIARDRHVEQALNETPEKLNRDRR
                     LVLLSDPVTMARLHFRVWNSPERYSSWVSYYEGIKLNPLALRKPDAASQ"
BASE COUNT         2123 a         1994 c         2191 g         2104 t
ORIGIN      
        1 agaccctctc attgcctgct aatcaaccca ttgctttgac gaagttgagt ttaaatattt
       61 ccccggaaga tcgggtgaaa atagttgtta ctgtttctga tggacaggca cttcatttat
      121 cacaacaatg gacgccctct tcagaaaagt cttaatttgt tgaaatatcg agcataagat
      181 gaatctggag agaatggtct gctgcgaatc agccaacctg aaagtatgga taacacaacc
      241 ttcaaggatg actaatcatt gaggaaatag aataaatgtt cagacctttt ttagactctc
      301 ttatgctcgg cagtttgttt tttcctttta ttgccattgc aggaagcacc gcgcaagggg
      361 gcgtgatcca tttttatggc caaattgtgg aaccggcatg tgacgtcagc acccagtcat
      421 cacccgtaga aatgaactgc ccacagaatg gttctattcc gggcaaaacc tactccagca
      481 aagcgttaat gagcggcaat gtcaaaaacg cgcaaatagc ctcagtaaaa gtgcagtatc
      541 ttgataaaca gaaaaagctg gctgtaatga acatcgaata taactaagtt tctgaacaac
      601 tcacgttgct gagcagaaaa atgcgattta accaaaaagc ctgctgtaca cttaagaaac
      661 aagaaggtgt aaggaggcgt tatgaaaacg cgtattcatg ttgtgcaggg tgatattacc
      721 aaactggccg ttgatgtgat tgtgaatgcg gctaatccgt cattaatggg aggcggcggc
      781 gtcgatgggg ccattcatcg cgcagcgggt ccggccctgc tggatgcctg tttaaaagtc
      841 aggcaacagc agggcgattg ccctacgggg catgccgtta ttacgcttgc aggcgatctt
      901 cccgctaaag ccgtagtaca caccgtcggg ccagtctggc gtggtgggga acaaaacgaa
      961 gaccagcttt tgcaggatgc ctatctgaat agcctacgac tggtggcggc aaacagctat
     1021 acgtcagtgg cttttcctgc aatcagtact ggggtttata gttaccctcg tgcggcagcg
     1081 gctgaaatcg cagttaaaac cgtttcagaa tttattaccc gtcacgcttt acccgaacag
     1141 gtatactttg tctgttatga tgaagaaaac gcccacctct acgaaagact ccttacccaa
     1201 caaggagatg aatgatttgc cccggctggc gagcgcggtg ctgccactgt gttcgcaaca
     1261 tcccggtcag tgtggtcttt tccctctgga gaaaagtctg gatgcgtttg ccgcccggta
     1321 tcgtctggcc gaaatggcag agcatacgct ggatgttcag tattacatct ggcaggacga
     1381 tatgtcgggg cggttactgt tttccgccct gttagccgca gcaaagcgtg gcgttcgcgt
     1441 ccgtttgttg ctggacgaca acaatacgcc tggacttgac gacattttgc gcttgcttga
     1501 cagtcatcca cgcattgaag tccggctttt taatcctttc tcgtttcgct tgctacgtcc
     1561 gcttggatat atcaccgact tttcccgtct caatcgccgg atgcacaata aaagtttcac
     1621 tgtcgatggc gtggttaccc tagtgggagg acgaaatatt ggtgatgcct attttggtgc
     1681 aggggaagag ccactttttt cggatttaga tgtcatggca ataggccccg tggtagagga
     1741 tgttgccgat gatttcgccc gctactggta ttgcaaatcg gtttcaccct tacagcaggt
     1801 gctggatgtc ccggaaggtg aaatggcgga tcgcatcgag ttacccgcct cctggcataa
     1861 cgatgccatg acgcatcgtt atttacgcaa aatggaatcc agtccattta taaatcatct
     1921 ggttgatgga acgttgccgc ttatctgggc gaagacacgt ttattaagtg atgatccggc
     1981 gaaaggggag ggcaaggcaa aacggcattc actgttaccg cagcgcctgt tcgatatcat
     2041 gggctcaccc agtgaacgca tcgatattat ctcttcctat tttgtaccga cacgcgcagg
     2101 tgtggcgcaa ctcttacgga tggtgagaaa aggggtaaag attgcgatcc taaccaattc
     2161 tcttgccgct aacgatgttg ctgtcgtcca tgccggatac gcgcgctggc gcaaaaaatt
     2221 gctccgctat ggcgtggaat tatatgaact caagccgacg cgtgaacaaa gcagtacgtt
     2281 acacgatcgc ggcataaccg gtaattccgg tgccagcctg catgctaaaa cttttagcat
     2341 cgatggtaaa acggtgttta tcggctcttt caatttcgat ccgcgttcaa cattgctcaa
     2401 tactgaaatg ggcttcgtga tagagagcga aacgctggca cagttaattg ataaacgctt
     2461 tattcagagc cagtacgatg cggcctggca gctccgtctg gacaggtggg gacggatcaa
     2521 ctgggttgat cgtcatgcaa agaaagaggt tattctcaaa aaagaacccg ccaccagttt
     2581 ctggaagcgg gttatggtca gactggcgtc gatattgccc gtggaatggt tattgtaaat
     2641 gtggcttaac gggctggtgc tttatcgttc tcacgcttaa caaccggttt accagagaac
     2701 aaaaacttca gtaacgggat gcgtaaatga atttcataca gaattatcgc aatccctact
     2761 acgaatatca ggccacagag aaaaccaagc cagttggagg tgatgtgcgg tgtaatgtat
     2821 gcgccgaaaa acagcgttaa cgggtggtga accagataga taaacagcga tgcgttaaca
     2881 aaataagtca cccgcgctga ctggaagtta agcaaacggt ggccaaagga gaagaccaca
     2941 ttcaccatcc acaggccgag gaccatggtg atcaccgact cggtttcgta catccaggca
     3001 tcgccactgc catagcgctg gttgagtaaa taagcgacaa acgccaatgc tgcagcgagg
     3061 gtacagccac gagacggcgt ggtaaacaag gctttaagat gagggaaaat gaaagccagt
     3121 gcgccgagga taaagaacgg taaataaaat agcgtttgca tgacaataaa attgaacatg
     3181 ccattactca gaatgggtgg atacacaata aaaatcgttc ttcttattac cgcatagccg
     3241 atgccgaggc ataaaaaaat caccgatagt tttaccatcg agaatttttt attcgtttta
     3301 tcagaatttt ctaaattatt tctgatgcgc ttaaatatcc atacacacag cgtcgtcatg
     3361 actactaaca ccagtaaaaa ccacaggtgt gatattaatt cccaggccaa cgtattatat
     3421 ttgtcataca atgatagccc aggccaactt tccgctttcc ctttgacata ttgcagcata
     3481 ataaattgcg gtaatgtcag tagggggatg gctgttaaca tcgggatacc tacacgttcg
     3541 acacgtactt tccaccattt tttcaaggga tagcgtaaaa aaagcatgta ggaaaagtat
     3601 ccggatataa cgaaaaatac ctgcatgcgg aacgagtgga tgaagtcatt aaaaagggtt
     3661 agccacaatg acggttcggc gctattcaca tgccatgtat ggctcgaata gattaaagaa
     3721 atatgaaaag ggatccctaa caacatcagc caggcgcgga tggagtcgag gaaatattca
     3781 cgttgcgcgg gtactggatt catatatggt taactaatct cggatttttc gtcttatccc
     3841 tgtcgggtta tgcctttagg cttgttgcca tagcgacacc gacctgaccg cgccaggcgc
     3901 aggcttcaag gtttttatgc atagcatcat cgctaccact aaccagaatg gaagcgtctg
     3961 taagacggtt gataaataaa tttgctggca aaccctacac gaagtcgatg cttctgtctt
     4021 taggagaagc acggaaagtg aaaacggttg caatcaggtg cttaatccat gagccagcgt
     4081 gctgaacgat accgggattc agttgtcgga atggctggtt atccattaaa atagatcgga
     4141 tcgatataag cacacaaagg gggaagtgct tactaattat gaaacataaa ctacaaatga
     4201 tgaaaatgcg ttggttgagt gctgcagtaa tgttaaccct gtatacatct tcaagctggg
     4261 ctttcagtat tgatgatgtc gcaaagcaag ctcaatcctt agccgggaaa ggctatgagg
     4321 cgcctaaaag caacttgccc tccgttttcc gcgacatgaa atacgcggac tatcagcaga
     4381 tccagtttaa tcatgacaaa gcgtactgga acaatctgaa gaccccattc aaactcgagt
     4441 tctaccatca gggtatgtac ttcgataccc cggtcaaaat aaatgaagtg actgccaccg
     4501 cagtcaaacg aatcaaatac agcccggatt atttcacttt cggcgatgtt cagcatgaca
     4561 aagacacggt aaaagacctt ggttttgccg gttttaaagt gctttacccg atcaacagca
     4621 aagataaaaa cgatgaaatc gtcagcatgc tcggggccag ctatttccgc gtgattggtg
     4681 caggtcaggt ttatggcctt tctgcccgcg gcctagcaat tgataccgct ttgccatcgg
     4741 gtgaagaatt tccacgcttc aaagagttct ggatcgagcg tccaaaaccg actgataaac
     4801 gtttaaccat ttatgcattg cttgactcgc cgcgtgcgac aggtgcttac aaattcgtgg
     4861 ttatgccagg gcgtgacacg gttgtggatg tgcagtcgaa aatctatctg cgcgataaag
     4921 tcggcaaact gggggttgca ccgttaacca gtatgttcct gtttgggccg aaccaaccgt
     4981 tgcctgcaaa taactatcgt ccggagttgc acgactctaa cggtctgtct atccatgctg
     5041 gtaatggcga atggatctgg cgtccgttga ataacccgaa acatttagcg gtcagcagct
     5101 tctccatgga aaacccgcga ggctttggtc tgttgcaacg aggtcgtgat ttctcccgct
     5161 ttgaagatct cgatgatcgt tacgatctcc gtccaagcgc atgggtgact ccgaaagggg
     5221 agtggggcaa aggcagcgtt gagctggtgg aaattccaac caacgatgaa accaacgata
     5281 acatcgtcgc ttactggacg ccggatcagc tgccggagcc gggtaaagag atgaacttta
     5341 aatacaccat caccttcagc cgtgatgaag acaaactgca tgcgccagat aacgcatggg
     5401 tgcaacaaac gcgtcgttca acgggggatg tgaagcagtc gaacctgatt cgccagcctg
     5461 acggtactat cgcctttgtg gtcgatttta ccggcgcaga gatgaaaaaa ctgccagagg
     5521 ataccccggt cacagcgcaa accagcattg gtgataatgg tgagatagtt gaaagcacgg
     5581 tgcgctataa cccggttacc aaaggctggc gtctggtgat gcgtgtgaaa gtgaaagatg
     5641 ccaagaaaat cactgaaatg cgtgctgcgc tggtgaatgc cgatcagacg ttgagtgaaa
     5701 cctggagcta ccagttacct gccaatgaat aagacaactg agtacattga cgcaatgccc
     5761 atcgccgcaa gcgagaaagc agcattgccg aagactgata tccgcgccgt tcatcaggcg
     5821 ctggatgccg aacaccgcac ctgggcgcgg gaggatgatt ccccgcaagg ctcggtaaag
     5881 gcgcgtctgg aacaagcctg gccagattca cttgctgatg gacagttaat taaagacgac
     5941 gaagggcgcg atcagcttaa ggcgatgcca gaagcaaaac gctcctcgat gtttcccgac
     6001 ccgtggcgta ccaacccggt aggccgtttc tgggatcgcc tgcgtggacg cgatgtcacg
     6061 ccgcgctatc tggctcgttt gaccaaagaa gagcaggaga gcgagcaaaa gtggcgtacc
     6121 gttggtacca tccgccgtta cattctgttg atcctgacgc tcgcgcaaac tgttgtcgcg
     6181 acctggtata tgaagaccat tcttccttat cagggttggg cgctgattaa tcctatggat
     6241 atggttggtc aggatttgtg ggtttccttt atgcagcttc tgccttatat gctgcaaacc
     6301 ggtatcctga tcctttttgc ggtactgttc tgttgggtgt ccgccggatt ctggacggcg
     6361 ttgatgggct tcctgcaact gcttattggt cgcgataaat acagtatatc tgcgtcaaca
     6421 gttggcgatg aaccattaaa cccggagcat cgcacggcgt tgatcatgcc tatctgtaac
     6481 gaagacgtga accgtgtttt tgctggcttg cgtgcaacgt gggaatcagt aaaagccacc
     6541 gggaatgcca aacactttga tgtctacatt ctgagtgaca gttataaccc ggatatctgc
     6601 gtcgcagagc aaaaagcctg gatggagctt atcgctgaag tcggtggcga aggtcagatt
     6661 ttctatcgcc gccgccgtcg ccgcgtgaag cgtaaaagcg gtaatatcga tgacttctgc
     6721 cgtcgctggg gcagccagta cagctacatg gtggtgctgg atgctgactc ggtaatgacc
     6781 ggtgattgtt tatgcggcct ggtgcgcttg atggaagcca acccgaacgc cgggatcatt
     6841 cagtcgtcgc cgaaagcgtc cggtatggat acgctgtatg cgcgctgtca gcagttcgcg
     6901 acccgcgtgt atgggccact gtttacagcc ggtttgcact tctggcaact tggcgagtcg
     6961 cactactggg gacataacgc gattatccgc gtgaaaccat ttatcgagca ctgcgcactg
     7021 gctccgctgc cgggcgaagg ttcctttgcc ggttcaatcc tgtcacatga cttcgtagaa
     7081 gcggcgttga tgcgccgtgc aggttggggg gtctggattg cttacgatct cccgggttct
     7141 tatgaagaat tgccgcctaa cttgcttgat gagctaaaac gtgaccgccg ctggtgccac
     7201 ggtaacctga tgaacttccg tctgttcctg gttaagggta tgcacccggt tcaccgtgcg
     7261 gtgttcctga cgggcgtgat gtcttatctc tccgctccgc tgtggtttat gttcctcgcg
     7321 ctctctactg cattgcaggt agtgcatgcg ttgaccgaac cgcaatactt cctgcaacca
     7381 cggcagttgt tcccggtgtg gccgcagtgg cgtcctgagc tggcgattgc actttttgct
     7441 tcgaccatgg tgctgttgtt cctgccgaag ttattgagca ttttgcttat ctggtgcaaa
     7501 ggaacgaaag aatacggcgg cttctggcgc gttacattat cgttgctgct ggaagtgctg
     7561 ttttccgtgc tgctggctcc ggtacgcatg ctgttccata cggtcttcgt tgtcagcgcg
     7621 ttccttggct gggaagtggt gtggaattca ccgcagcgtg atgatgactc cacttcatgg
     7681 ggcgaagcgt tcaaacgcca cggctcacag ctgctgttgg ggttagtgtg ggctgttggg
     7741 atggcgtggc tggatctgcg tttcctgttc tggctggcac cgattgtctt ctcgttgatc
     7801 ctgtcaccgt ttgtttcggt gatttccagc cgtgccaccg ttggtctgcg caccaaacgc
     7861 tggaaactgt tcctgatccc ggaagagtat tcaccgccgc aggtgctggt tgataccgat
     7921 cggttccttg agatgaatcg ccaacgctcc cttgacgatg gctttatgca cgcagtgttt
     7981 aacccgtcat ttaacgctct ggcaaccgca atggcgacag cgcgtcaccg cgccagtaag
     8041 gtgctggaaa tcgcccgtga ccgccacgtt gaacaggcgc ttaacgagac gccagagaag
     8101 ctgaatcgcg atcgtcgcct ggtgctgcta agcgatccgg tgacgatggc ccgtctgcat
     8161 ttccgcgtct ggaattcccc tgaaagatat tcttcatggg tgagttatta cgaagggata
     8221 aagctcaatc cactggcatt gcgtaaaccg gatgcggctt cgcaataaaa acgtagttgc
     8281 ctgatgcgct acgcttatca ggcctacatc gttcctgcaa tttattgatt ttgcaagatt
     8341 ttgtaggtcg gataaggcgt tcacgccaca tccggcaaaa acaacgagcc aataaaaata
     8401 ccggcgttat gc
//
LOCUS       DAJTRC010000142         8333 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.142, whole genome shotgun sequence.
ACCESSION   DAJTRC010000142 DAJTRC010000000
VERSION     DAJTRC010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8333)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8333)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8333
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.142"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..158
                     /locus_tag="OO357_003639"
     CDS             <1..158
                     /locus_tag="OO357_003639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000009331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888449.1"
                     /translation="SESGEVVDMAAYEAWQQENHPDTWQQMQRREEVNINVHRERGED
                     VEPGDDF"
     gene            complement(167..565)
                     /gene="vapC"
                     /locus_tag="OO357_003640"
     CDS             complement(167..565)
                     /gene="vapC"
                     /locus_tag="OO357_003640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA(fMet)-specific endonuclease VapC"
                     /protein_id="HCR5888450.1"
                     /translation="MLKFMLDTNICIFTIKNKPASVRERFNLNQGKMCISSVTLMELI
                     YGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHTGQIRAELARQGRPVGPFDQMIA
                     GHARSRGLIIVTNNTREFERVGGLRTEDWS"
     gene            complement(565..792)
                     /gene="vapB"
                     /locus_tag="OO357_003641"
     CDS             complement(565..792)
                     /gene="vapB"
                     /locus_tag="OO357_003641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin-antitoxin system antitoxin VapB"
                     /protein_id="HCR5888451.1"
                     /translation="METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAG
                     ETWDEWFDGHSVSTDFMDNREQPGMQERESF"
     gene            874..1245
                     /locus_tag="OO357_003642"
     CDS             874..1245
                     /locus_tag="OO357_003642"
                     /inference="COORDINATES: protein motif:HMM:NF020332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative relaxase"
                     /protein_id="HCR5888452.1"
                     /translation="MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQ
                     GSVDKDVFTRLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSISMMAMLGGDKRL
                     IEAHNQAVDFAVRQVEASAST"
     gene            1266..6127
                     /gene="traI"
                     /locus_tag="OO357_003643"
                     /pseudo
     CDS             1266..6127
                     /gene="traI"
                     /locus_tag="OO357_003643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005079803.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugative transfer relaxase/helicase TraI"
     gene            6147..6893
                     /gene="traX"
                     /locus_tag="OO357_003644"
     CDS             6147..6893
                     /gene="traX"
                     /locus_tag="OO357_003644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858383.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer pilus acetylase TraX"
                     /protein_id="HCR5888453.1"
                     /translation="MTTDNTNTTRNDSLAARTDTWLQSFLVWSPGQRDIIKTVALVLM
                     VLDHINLIFQLKQEWMFLAGRGAFPLFALVWGLNLSRHAHIRQPAINRLWGWGIIAQF
                     AYYLAGFPWYEGNILFAFAVAAQVLTWCETRSGWRTAAAILLMALWGPLSGTSYGIAG
                     LLMLAVSNRLYRAEDRAERLALVACLLAVIPALNLATSDAAAVAGLVMTVLTVGLVLC
                     AGKSLPRFWPGDFFPTFYACHLAVLGVLAL"
     gene            6948..7508
                     /locus_tag="OO357_003645"
     CDS             6948..7508
                     /locus_tag="OO357_003645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fertility inhibition protein FinO"
                     /protein_id="HCR5888454.1"
                     /translation="MTEQKRPVLTLKRKTEGTAPVRSRKTIINVTTPPKWKVKKQKLA
                     EKAAREAELAAKKAQARQALSIYLNLPTLDEAVNTLKPWWPGLFDGDTPRLLACGIRD
                     VLLEDVAQRNIPLSHKKLRRALKAITRSESYLCAMKAGACRYDTEGYVTEHISQEEEA
                     YAGARLAKIRRQNRIKAELQAVLDEK"
     gene            7639..7851
                     /locus_tag="OO357_003646"
     CDS             7639..7851
                     /locus_tag="OO357_003646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANR family transcriptional regulator"
                     /protein_id="HCR5888455.1"
                     /translation="MNGFRNSSRNGQVWRYQRAGGRAVILEVSGRWMEAAEAWRRAAC
                     VAPRTDWQQFARKRAEHCHRRCRGRV"
     gene            8095..>8333
                     /locus_tag="OO357_003647"
     CDS             8095..>8333
                     /locus_tag="OO357_003647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5888456.1"
                     /translation="MRKYIPLALFIFSWPVLSADIHGRVVRVLDGDTIEVMDSLKAVR
                     IRLVNIDAPEKKQDYGRWSTDMMKSLVAGKTVTVT"
BASE COUNT         1913 a         2085 c         2671 g         1664 t
ORIGIN      
        1 tcagtgaatc cggtgaagtg gtggatatgg ccgcttatga ggcatggcag caggaaaatc
       61 atccggacac ctggcagcag atgcagcgtc gtgaagaggt gaacatcaat gtgcaccggg
      121 aacgcgggga ggatgttgag ccgggagatg atttctgatg aacaggtcag ctccagtctt
      181 cagttctcag gccgcccaca cgttcaaatt cccgggtgtt attagtcaca ataatcagcc
      241 cccgactgcg ggcatgacct gcgatcattt gatcaaatgg cccgacaggg cgtccctgac
      301 gggcaagttc tgctcttatc tggccggtgt gtgtggctgc agcagcgtcg taatccagaa
      361 catcaatgcg ggaaacaaat ccctcgatca cagcgagatt acgttcaggc atctggcttt
      421 tttctgcacc atatatcagc tccatcaggg tgaccgaact gatacacatt ttcccctggt
      481 tcaggttaaa acgctccctg acgctggcgg gtttgttctt tatcgtaaaa atgcagatgt
      541 tggtatcgag cataaacttc agcatcagaa tgactccctt tcttgcatgc cgggctgttc
      601 cctgttatcc ataaaatcgg tgctgacgct gtgtccgtcg aaccattcgt cccatgtctc
      661 tccggctggc gtaatgattc tggttcgtcc gacagcaatc acttcaacgc gttttacgtt
      721 ttccggcaat gcaaccgctt ttggcagtct gaccgcctgg ctgcggttgc tgagaaatac
      781 ggtggtttcc attatttgcc tccttatgtg gatatgtctt gtgtatatct attgtgggat
      841 tgataccggg atatgtcaaa gggatatacg tttatgatga gtatcgcgca ggtcagatcg
      901 gccggaagtg ccggtaatta ttataccgac aaggataatt actatgtgct gggcagcatg
      961 ggagaacgct gggccggcca gggggctgaa caactggggc tgcagggcag tgtcgataag
     1021 gatgtcttta cccgccttct ggagggcagg ctgccggacg gggcggattt aagccgcatg
     1081 caggatggca gtaacaggca tcgtcccggc tacgacctga ccttctccgc tcccaaaagt
     1141 atctccatga tggccatgct cgggggggac aaacgtctta ttgaagcaca taaccaggcc
     1201 gtggattttg ctgtccgtca ggtggaggcc agtgcctcca cctgacggac ggacagtcag
     1261 aaacggtgct gaccggtaat ctggtgatgg cactgtttaa ccacgacacc agtcgcgatc
     1321 aggaaccaca gttacacacg catgcggtgg tgactaatgt tacgcagtat aacggcgaat
     1381 ggaagacact gagcagtgac aaagtgggga aaacggggtt cattgagaat gtgtacgcta
     1441 atcagattgc ctttggcagg ctctaccggg aaaaactgaa agagcaggtt gaggcgctgg
     1501 gctatgaaac tgaagtggtc ggtaagcacg gtatgtggga aatgccgggt gtaccggtgg
     1561 aggccttttc cggacgctca cagactatcc gggaggccgt cggggaagac gcctcgctga
     1621 aatcccggga tgtggcggcg ctggatacgc gtaaatccaa acagcacgtc gatcctgaag
     1681 tcagaatggc cgagtggatg cagacgctga aggaaaccgg gttcgacatc cgggcatatc
     1741 gtgacgcagc ggaacaacgg gcatataccc gcacgcagac acccggaccc gcttcacagg
     1801 acgggccgga tgtgcagcag gcggtgacac aggccattgc cggattaagt gaacgcaaag
     1861 tgcagtttat gtatacggac ctgctggcca ggacggtcgg catactgccg ccggaaaatg
     1921 gtgtgattga acgggcacgc gccggtatcg atgaggccat cagccgtgag cagcttatcc
     1981 ccctcgatcg tgagaagggg ctgttcacgt tcgggattca tatgctcgat gagctgtcag
     2041 tccgggcact cagtcgtgac atcatgaaac agaaccgggt gaccgtacat ccggagaaaa
     2101 gtgtcccccg gacggccggt tacagcgatg ccgtcagtgt tctggcacag gaccgtccgt
     2161 cgctggccat tgtgtccggg cagggcggtg cagccgggca gcgtgagcgg gtggctgaac
     2221 tggtcatgat ggcccgggag caggggcggg aggtgcagat tatcgctgct gaccgtcgct
     2281 cgcagatgaa cctgaagcag gatgaacggc tgtccggtga actgataacc ggacgtcgtc
     2341 agttgctgga aggaatggcc ttcacgccgg gcagtaccgt tatcgtcgac cagggcgaaa
     2401 aactctccct gaaagagacg ttaaccctgc tggatggtgc cgcacgtcat aacgtacagg
     2461 tcctgataac cgacagcggg cagcgaaccg gtacaggcag tgcactgatg gccatgaagg
     2521 atgccggggt gaacacatat cgctggcagg ggggagaaca gcgaccggcc accatcatca
     2581 gtgaaccgga ccgtaatgtc ggctggccgg agattttgcg gccagcgtga aagccggaga
     2641 agagagcgtg gcacaggtca gcggagtacg ggaacaggcc atactgacac aggccattcg
     2701 cagtgagctg aaaacacagg gcgtgctcgg acacccggag gtaaccatga ccgccctgtc
     2761 accggtctgg ctggacagcc ggagccgtta tctgcgggat atgtaccgcc cgggaatggt
     2821 gatggagcag tggaacccgg agacacgcag tcatgaccgc tatgtgacag aacgtgtgac
     2881 ggcgcagagt cacagcctga ccctgcggaa tgcgcagggc gaaacgcagg tggtgcgtat
     2941 ttcttccctg gacagcagct ggtcgctgtt ccggccggaa aaaatgccgg tggcagacgg
     3001 tgagcgtctg agggtgacag ggaaaattcc cggactccgc gtctccggcg gtgaccgcct
     3061 gcaggtggca tccgtcagtg aagatgcgat gacggttgtt gtgccgggac gggctgaacc
     3121 ggccaccctg cctgtgagcg attcaccgtt cactgcactg aagctggaga acggctgggt
     3181 ggaaacgccc gggcattccg tcagcgacag tgcgacggtt tttgcctccg tcacacagat
     3241 ggcaatggac aatgccaccc tgaatggtct ggcccgcagt ggtcgtgatg tccggctgta
     3301 ttcctcactg gatgaaaccc gtactgcgga aaaacttgcc cgccatccct cctttacggt
     3361 ggtttctgag cagataaagg cgcgtgccgg tgagacattg ctggaaaccg ctatcagtct
     3421 gcagaaagcc gggcttcaca cgccggcaca gcaggccatt catctggccc ttcctgtgct
     3481 ggaaagtaaa aacctggcct tcagcatggt ggacctgctg acagaggcga agtcgtttgc
     3541 tgcagaagga accggttttg ctgacctggg aggggaaatc aatgcgcaga taaaacgggg
     3601 tgatttactg tatgtggatg tggcaaaagg ctatggcaca ggcctgctgg tttcccgtgc
     3661 gtcgtatgag gcagaaaaga gcattcttcg ccatattctc gaaggtaagg aggcggtcac
     3721 gccgctgatg gagagagtac ctggcgaact catggagaaa ctgacatcag gacagcgcgc
     3781 cgccacccgt atgatactgg aaacgtctga ccgtttcacg gtggtgcagg gctatgccgg
     3841 tgtgggtaag accacacagt tccgggcggt gatgtcagcc gtgaacatgc tgccggagag
     3901 tgaacgtccc cgtgtcgtgg ggctggggcc cacacaccgt gcggtcgggg agatgcgcag
     3961 cgccggcgtg gatgcacaga cactggcgtc ctttctgcat gacacgcagc tgcagcagcg
     4021 cagcggagaa actccggatt tcagcaacac gctgttcctt ctcgatgaga gctctatggt
     4081 gggcaatacc gacatggcac gggcatacgc cctgattgcg gccggtggcg gtcgggctgt
     4141 ggccagcggt gatacggacc agctgcaggc catcgcgccc ggtcagcctt tccgtctcca
     4201 gcagacgcgc agtgctgccg atgtggtcat catgaaggag attgtgcgtc agacgccgga
     4261 actgcgggag gcggtataca gcctgattaa ccgggatgtg gaaagggcgc tgtccgggct
     4321 tgagcgtgtg aaaccgtctc aggtgccacg tctggagggc gcatgggcac cggagcactc
     4381 cgtgacggag ttcagtcaca gccaggaagc gaaactggca gaagcgcagc agaaggcgat
     4441 gctgaaaggc gaggcttttc cggatgtccc catgacactg tatgaagcca ttgtccgcga
     4501 ctataccggc aggacaccgg aagcacggga gcagacgctg attgtcacgc acctgaatga
     4561 ggaccggcgc gtactgaaca gcatgattca tgatgcacgg gaaaaggccg gagaactggg
     4621 gaaggtgcag gtcatggtgc ctgtcctgaa cacagcgaat atacgcgacg gggagctgcg
     4681 tcgtctctcc acctgggaga ataacccgga tgcccttgcc ctggtggata atgtgtatca
     4741 ccggattgcc ggtatcagta aggatgacgg gctgataacc ctgcaggatg cggaaggtaa
     4801 cacgcggctg atttcgcccc gggaggcggt ggctgaaggc gtcacgctgt acaccccgga
     4861 caccatccgg gtggggaccg gtgacaggat acgcttcacg aagagtgacc gggagcgcgg
     4921 ttatgtggcc aacagcgtct ggacggtgac agcggtttcc ggtgacagtg tcacactttc
     4981 tgacggtcag cagacccgtg tgattcgccc cggtcaggag cgggcagagc aacatattga
     5041 cctggcctat gccatcaccg cccacggtgc acagggggcg agtgaaacct ttgccatcgc
     5101 gcttgaaggc acggaaggta accggaaact gatggccggc tttgagtcag cctacgtggc
     5161 cctgtcgcgt atgaagcagc atgtgcaggt gtacaccgat aaccgtcagg gctggacgga
     5221 tgccatcaac aatgccgtac agaaaggaac tgcccacgat gtgtttgagc cgaaaccgga
     5281 ccgggaggtc atgaatgcag agcggctgtt cagtacggcg cgggagctgc gggacgtggc
     5341 ggcaggccgt gccgttctcc gtcaggcggg gctggccggg ggagacagtc ctgcacggtt
     5401 tattgctccg ggacgtaaat atccgcaacc gtatgtggca ctgccggcgt ttgaccgtaa
     5461 cggcaagtcc gccggtatct ggctgaaccc gctgaccacg gatgacggaa acgggctgcg
     5521 gggattcagc ggtgaaggtc gggtaaaagg cagcggggat gcgcagttcg tggccctgca
     5581 gggcagccgt aacggagaaa gcctgctggc tgataatatg caggatggcg tccggattgc
     5641 ccgtgataat cctgacagtg gtgtggtggt aagaatcgcc ggtgaaggcc gtccgtggaa
     5701 tcccggtgcc atcaccggtg gccgcgtgtg gggggatatc ccggacaaca gcgtccagcc
     5761 gggagccgga aatggtgaac cggtcacggc agaggtactg gcacagcggc aggctgaaga
     5821 ggccatccgc cgtgaaacgg aacgtcgcgc agatgaaatt gtccgtaaaa tggcagagaa
     5881 caaacctgac ctgccggacg gcaaaacaga gcaggctgtc agggaaattg ccgggcagga
     5941 gcgtgaccgg gctgccataa ctgaacggga agccgcgctg ccggagagtg tactgcgtga
     6001 accacaacgg gtgcgggaag cggtccggga agttgcccgg gaaaatttgc tgcaggagcg
     6061 tctgcagcag atggagcggg atatggttcg tgacctgcag aaagagaaaa ccccgggcgg
     6121 agactgatac aggaagacaa acactgatga caacggataa cacgaacacg acacgtaacg
     6181 attcactggc tgcacggacc gatacctggt tgcagtcatt tctggtctgg tcacccggac
     6241 agcgggatat catcaaaacg gtggcactgg tgctgatggt actggaccac atcaatctga
     6301 tattccagct gaagcaggaa tggatgtttc tggccgggcg aggagccttt ccgctttttg
     6361 ccctggtgtg ggggctgaat ctgtcccgtc atgcgcatat ccggcaaccg gccattaacc
     6421 ggctgtgggg atggggaatt attgcccagt tcgcatatta tctggcaggc tttccttggt
     6481 atgaggggaa tatcctgttt gcctttgcag tggcggcaca ggtgctgacg tggtgtgaaa
     6541 cgcgcagtgg gtggcgtaca gcagcggcca tactcctgat ggccctgtgg gggcctttgt
     6601 ccggcaccag ttacggtatt gccgggttgc tgatgctggc agtcagcaac cggctgtacc
     6661 gggcggaaga cagagcggaa cgtctggcgc tggtcgcctg cctgctggct gttattccgg
     6721 cgcttaacct tgccaccagt gatgcagcgg cggtggccgg tcttgtgatg acggtgctga
     6781 ccgttggtct ggtgttgtgt gccggaaaat cattaccccg tttctggccc ggagactttt
     6841 tcccgacgtt ctatgcctgt catctggctg tgctgggcgt tctggcgctg tgacgggtgt
     6901 ggtatctttg gccgcaagag gatgattcgt cagaggcaga acacagcatg acagagcaga
     6961 agcgaccggt actgacactg aagcggaaga cagagggaac agcgcctgtc cgcagccgga
     7021 aaaccatcat caatgtcacc acgccaccaa agtggaaggt gaaaaagcag aagctggccg
     7081 agaaagcggc ccgtgaagca gagctggcgg caaaaaaagc gcaggccaga caggcgctgt
     7141 ccatttatct gaacctgccc acgctggatg aggctgtgaa taccctgaag ccctggtggc
     7201 cgggattatt tgacggtgac acgccccggc ttctggcctg cggtatccgg gacgtgttac
     7261 tggaagacgt ggcgcagcgg aatatcccgc tctcgcataa aaaactgcgc agggcgctga
     7321 aggccatcac ccgttcagaa agctatctgt gtgccatgaa agccggtgcc tgccggtatg
     7381 acacggaagg gtatgtgacg gagcatattt ctcaggagga ggaagcgtat gcgggagcgc
     7441 gtctggcgaa aattcgtcgc cagaaccgga taaaggcaga acttcaggcc gtgcttgatg
     7501 agaaataaaa aaagcctccc ccagagagag gaggcaggga aataaggctt aaaaggaaat
     7561 aacttccgct tccgaaagga agccagtcca ctgaattatt tatcacaata atactgacaa
     7621 ctaaaggggg acaagaaaat gaatggattc agaaacagtt cacggaacgg ccaggtctgg
     7681 cgttaccagc gtgccggcgg gcgggccgtt attctggaag tcagtggacg ctggatggaa
     7741 gcggcagaag catggcgacg ggctgcctgt gttgcgccta ggacagactg gcagcagttt
     7801 gcccggaaaa gggctgaaca ctgccaccgg cgctgccggg gaagggtgta acgaaaaaag
     7861 cagccttcat tactctagga gtcagaaggc tgaaaggagt ttatgagatt tgcttcttaa
     7921 tatccggtcg ggcagtatga ccggacagtt ctgagtagca cagagtcttt tccgcattaa
     7981 gtcgattttt tatattttgt gattagtttc acaaaataag gtgttattca atgtgtgctg
     8041 cactattcag gggggctgaa atacaggtgg catttatctg atatggagaa taacatgagg
     8101 aaatatattc cactggcatt atttatcttt tcatggccgg tattgtctgc agatattcat
     8161 ggacgggttg ttcgtgtact ggacggtgat accatcgagg tcatggactc actgaaggcg
     8221 gtgcggatca ggctggtaaa tattgatgca ccggaaaaga aacaggatta tggccggtgg
     8281 tcaacagaca tgatgaaatc actggtcgca ggaaaaacgg ttaccgtcac ata
//
LOCUS       DAJTRC010000143         8168 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.143, whole genome shotgun sequence.
ACCESSION   DAJTRC010000143 DAJTRC010000000
VERSION     DAJTRC010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8168)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8168)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8168
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.143"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..2152)
                     /gene="uvrD"
                     /locus_tag="OO357_003648"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
     CDS             complement(<1..2152)
                     /gene="uvrD"
                     /locus_tag="OO357_003648"
                     /gene_synonym="mutU"
                     /gene_synonym="recL"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase II"
                     /protein_id="HCR5888457.1"
                     /translation="MDVSYLLDSLNDKQREAVAAPRSNLLVLAGAGSGKTRVLVHRIA
                     WLMSVENCSPYSIMAVTFTNKAAAEMRHRIGQLMGTSQGGMWVGTFHGLAHRLLRAHH
                     MDANLPQDFQILDSEDQLRLLKRLIKAMNLDEKQWPPRQAMWYINSQKDEGLRPHHIQ
                     SYGNPVEQTWQKVYQAYQEACDRAGLVDFAELLLRAHELWLNKPHILQHYRERFTNIL
                     VDEFQDTNNIQYAWIRLLAGDTGKVMIVGDDDQSIYGWRGAQVENIQRFLNDFPGAEI
                     IRLEQNYRSTSNILSAANALIENNNGRLGKKLWTDGADGEPISLYCAFNELDEARFVV
                     NRIKTWQDNGGALAECAILYRSNAQSRVLEEALLQASMPYRIYGGMRFFERQEIKDAL
                     SYLRLIANRNDDAAFERVVNTPTRGIGDRTLDVVRQTSRDRQLTLWQACRELLQEKAL
                     AGRAASALQRFMELIDALAQETADMPLHVQTDRVIKDSGLRTMYEQEKGEKGQTRIEN
                     LEELVTATRQFSYNEEDEDLMPLQAFLSHAALEAGEGQADTWQDAVQLMTMHSAKGLE
                     FPQVFIVGMEEGMFPSQMSLDEGGRLEEERRLAYVGVTRAMQKLTLTYAETRRLYGKE
                     VYHRPSRFIGELPEECVEEVRLRATVSRPVSHQRMGTPMVENDSGYKLGQRVRHAKFG
                     EGTIVNMEGSGEHSRLQVAFQGQGIKWLVAAYARL"
     gene            complement(2236..2952)
                     /gene="yigB"
                     /locus_tag="OO357_003649"
     CDS             complement(2236..2952)
                     /gene="yigB"
                     /locus_tag="OO357_003649"
                     /EC_number="3.1.3.104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(5-phospho-D-ribitylamino)uracil
                     phosphatase YigB"
                     /protein_id="HCR5888458.1"
                     /translation="MRFYRPLGRISALTFDLDDTLYDNRPVILRTEREALTFVQNYHP
                     ALRSFQNEDLQRLRQAVREAEPEIYHDVTRWRFRSIEQAMLDAGLSAEEASAGAHAAM
                     INFAKWRSRIDVPQQTHDTLKQLAKKWPLVAITNGNAQPELFGLGDYFEFVLRAGPHG
                     RSKPFSDMYFLAAEKLNVPIGEILHVGDDLTTDVGGAIRSGMQACWIRPENGDLMQTW
                     DSRLLPHLEISRLASLTSLI"
     gene            complement(2952..3848)
                     /gene="xerC"
                     /locus_tag="OO357_003650"
     CDS             complement(2952..3848)
                     /gene="xerC"
                     /locus_tag="OO357_003650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HCR5888459.1"
                     /translation="MTDLHTDVERYLRYLSVERQLSPITLLNYQRQLEAIINFASENG
                     LQSWQQCDAAMVRNFAVRSRRKGLGAASLALRLSALRSFFDWLVSQNELKANPAKGVS
                     APKAPRHLPKNIDVDDMNRLLDIDINDPLAVRDRAMLEVMYGAGLRLSELVGLDIKHL
                     DLESGEVWVMGKGSKERRLPIGRNAVAWIEHWLDLRDLFGSEDDALFLSKLGKRISAR
                     NVQKRFAEWGIKQGLNNHVHPHKLRHSFATHMLESSGDLRGVQELLGHANLSTTQIYT
                     HLDFQHLASVYDAAHPRAKRGK"
     gene            complement(3845..4552)
                     /locus_tag="OO357_003651"
     CDS             complement(3845..4552)
                     /locus_tag="OO357_003651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF484 domain-containing protein"
                     /protein_id="HCR5888460.1"
                     /translation="MKQPGEELQETLTELDDRAVVDYLIKNPEFFIRNARAVEAIRVP
                     HPVRGTVSLVEWHMARARNHIHVLEENMALLMEQAIANEGLFYRLLYLQRSLTAASSL
                     DDMLMRFHRWARDLGLAGASLRLFPDRWRLGAPSNHTHLALSRQSFEPLRIQRLGQEQ
                     HYLGPLNGPELLVVLPEAKAVGSVAMSMLGSDADLGVVLFTSRDASHYQQGQGTQLLH
                     EIALMLPELLERWIERV"
     gene            complement(4549..5373)
                     /gene="dapF"
                     /locus_tag="OO357_003652"
     CDS             complement(4549..5373)
                     /gene="dapF"
                     /locus_tag="OO357_003652"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005133314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HCR5888461.1"
                     /translation="MQFSKMHGLGNDFMVVDAVTQNVFFSPELIRRLADRHLGVGFDQ
                     LLVVEPPYDPELDFHYRIFNADGSEVAQCGNGARCFARFVRLKGLTNKRDIRVSTANG
                     RMVLTVTDDDLVRVNMGEPNFEPSAVPFRANKAEKTYIMRAAEQTILCGVVSMGNPHC
                     VIQVDDVDTAAVETLGPVLESHERFPERANIGFMQVVKREHIRLRVYERGAGETQACG
                     SGACAAVAVGIQQGLLDEEVRVELPGGRLDIAWKGPGHPLYMTGPAVHVYDGFIHL"
     gene            complement(5410..5613)
                     /locus_tag="OO357_003653"
     CDS             complement(5410..5613)
                     /locus_tag="OO357_003653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HCR5888462.1"
                     /translation="MKNVFKALTVLLTLFSLTGCGLKGPLYFPPADKNAPPPTKPVET
                     QTQSTVPDKNDRATGDGPSQVNY"
     gene            5802..7280
                     /locus_tag="OO357_003654"
                     /pseudo
     CDS             5802..7280
                     /locus_tag="OO357_003654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001659238.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
     gene            7277..>8168
                     /locus_tag="OO357_003655"
     CDS             7277..>8168
                     /locus_tag="OO357_003655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001584058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DKNYY family protein"
                     /protein_id="HCR5888463.1"
                     /translation="MKGQIITVALILLGHTFLTPAVQAIGFDYYNDHGVMSYGKGYWG
                     DKKIIAQFPEMSRAGLRLVKNISGKTGYIPSDEISIENEEYHWVTDGRVILWRGKIVS
                     NPPGTLTVDIASFQAMGRFAVDKYSLYFDGQRTESNSGASRVELATLKAIEGNSTTLV
                     DSKNLYLSGRRQGSSSDVTVLEKRWWGINPRLMSVNRNSYSNDLLIRSGQNIYLNGVH
                     LTANADSFEIIRWIPHSLLVFRDNKGLHRYPFGQLSGKAIPVDDDVSFEVGESRVRWR
                     KQLTPDRQWSKWIDLPGIEPE"
BASE COUNT         1884 a         2268 c         2099 g         1917 t
ORIGIN      
        1 ccagccgggc gtaagctgcc accagccatt tgattccctg gccctggaat gccacctgca
       61 aacggctatg ttcaccgctg ccttccatat tgacgatggt gccttcacca aacttagcgt
      121 ggcgtacacg ctggccgagc ttgtagccgc tgtcgttctc gaccatcggc gtacccatcc
      181 gctgatggct gaccgggcgg cttaccgtgg cgcgcaggcg cacctcttcc acacactctt
      241 ccggcaactc gccaataaag cgcgacgggc gatggtaaac ctctttgcca tacagacggc
      301 gagtttccgc gtaggtcagc gtcagtttct gcatcgcgcg ggttacgcca acgtaggcca
      361 gacggcgttc ttcttccaga cgcccgcctt catccagcga catctggctt gggaacatgc
      421 cctcttccat accgacgata aacacctgcg ggaactccag acctttcgcc gagtgcatgg
      481 tcatcagttg taccgcatcc tgccaggtat ccgcctgccc ttcgcctgct tccagcgccg
      541 catgggatag gaacgcctgc agcggcatta aatcttcgtc ttcttcgttg tagctgaact
      601 ggcgcgttgc cgtcaccagt tcctctaagt tttcgatacg cgtctgacct ttttcgccct
      661 tctcctgctc gtacatggta cgcaggccgg agtctttaat tacccggtca gtctgtacat
      721 gcagcggcat atcggcagtt tcctgcgcta aggcgtcgat cagttccata aaccgctgta
      781 aggcgctggc agcacgtccg gcgagggctt tttcctgcaa cagttcacga catgcctgcc
      841 agagtgttaa ctggcgatcg cgcgatgtct gacgtaccac gtccagcgtc cggtcaccaa
      901 taccccgcgt tggcgtattc accacacgct caaaggccgc gtcgtcgtta cggttggcaa
      961 tcaggcgcag atacgagagc gcatctttga tttcctggcg ttcgaagaag cgcatcccgc
     1021 cgtaaatacg gtacggcata ctcgcctgca acagtgcttc ttccagcaca cgcgactggg
     1081 cgttactgcg gtagagaatg gcgcactcgg caagcgcccc gccgttgtcc tgccaggttt
     1141 tgatgcggtt aaccacaaaa cgcgcttcat cgagttcgtt aaaagcgcaa tagagggaaa
     1201 taggctcacc gtccgcgcca tcggtccaca gttttttacc cagacgcccg ttattgtttt
     1261 caatcagggc gttagcggcg ctcagaatat tgctggtaga gcggtagttt tgctccagac
     1321 gaataatttc ggcaccgggg aaatcattaa ggaaacgctg aatattctcc acctgcgccc
     1381 cgcgccagcc gtagattgac tggtcgtcat caccgacgat catcactttg ccggtgtcgc
     1441 ccgccagcag gcggatccac gcgtactgaa tgttgttggt atcctggaat tcgtccacca
     1501 ggatattggt aaaacgttcg cggtagtgtt gtaggatatg cggcttgtta agccacagtt
     1561 cgtgagcgcg cagcagcagc tcggcgaagt ccaccaggcc cgcgcgatcg cacgcttcct
     1621 gatacgcctg atacaccttc tgccaggtct gctccaccgg attaccgtag ctttgaatat
     1681 gatgcggacg caggccttca tctttctggc tgttgatgta ccacattgcc tggcgcggcg
     1741 gccactgctt ctcgtcgagg ttcatggctt tgatcagacg cttaagcagg cgtagctggt
     1801 cttcgctatc gaggatctgg aaatcctgcg gcagattggc gtccatatgg tgcgcacgca
     1861 gcaggcggtg cgccagcccg tggaaggtgc ctacccacat gccgccctgg ctggtgccca
     1921 tcagttgtcc gatacgatga cgcatctccg ccgccgcttt gttggtaaac gtcacagcca
     1981 taatcgagta tggcgagcag ttttccacgc tcatcagcca ggcgatacga tgcaccagta
     2041 cgcgcgtctt accactgccc gcgcccgcca gcaccagaag gttgctgcgt ggcgcggcca
     2101 ccgcttcgcg ttgtttgtca ttaaggctgt cgagcaggta agaaacgtcc attggcaccg
     2161 ccgcgtaaaa ataggttggg cggagaagcg caacgccctc cgccaaaaag ctgggtatat
     2221 atacagattt gctgattata tcagcgaggt cagagatgcc aaccgggaaa tttccagatg
     2281 tggcagtaaa cggctgtccc aggtttgcat cagatcgcca ttttccggtc tgatccaaca
     2341 agcctgcatt ccgctgcgaa ttgccccacc cacgtcagtg gtgagatcgt ccccaacatg
     2401 taagatctcg ccgatcggca cgttgagttt ttccgcagcc aaaaagtaca tatcgctgaa
     2461 cggttttgag cgcccgtgcg ggccagcgcg cagcacaaac tcaaaataat cccccaaacc
     2521 aaacagttcc ggctgggcgt taccgttggt gatcgccacc agcggccatt tcttcgccag
     2581 ctgttttaag gtgtcgtgag tttgctgcgg gacgtcgatt cggctgcgcc atttggcaaa
     2641 gttaatcatt gctgcgtgtg cgcctgcact ggcttcttcg gcactcagcc cggcgtcgag
     2701 catcgcttgt tcaatcgaac gaaaacgcca acgtgtcacg tcgtgataaa tctcgggttc
     2761 cgcttcccgt accgcctggc gcaggcgttg cagatcttca ttctggaagc tgcgcagcgc
     2821 cggatgataa ttttgcacaa aggtaagcgc ctctcgctcg gtgcgcaaaa tcaccggacg
     2881 gttatcgtaa agggtatcat ccaggtcaaa ggtgagcgcc gagatgcgcc ccaaaggccg
     2941 gtaaaaacgc attatttccc ccgtttggcg cgtggatgcg ccgcatcgta caccgaggca
     3001 aggtgttgaa aatcaagatg agtatagatt tgcgtggtgg agaggttggc atgacccagt
     3061 agctcctgca caccacgaag atcgccgctc gactccagca tatgcgtagc gaacgagtga
     3121 cgtaatttat gtgggtgaac gtgattattc agcccttgtt ttatgcccca ttcggcaaag
     3181 cgtttctgca cattacgcgc ggagatacgc ttgcccagtt tcgacagaaa aagcgcgtcg
     3241 tcttcgctac caaacaggtc gcgcaaatca agccagtgct caatccacgc cacagcgttg
     3301 cgaccaatcg gcaggcggcg ctctttgctg cctttcccca tcacccacac ttcgccggac
     3361 tccaggtcga ggtgtttgat atccagcccc accagctcgg aaagacgcag acccgcgccg
     3421 tacatcactt ccagcattgc acggtcgcgt acagcgaggg gatcgttgat atcaatatcc
     3481 agcagccgat tcatatcgtc gacgtcgata tttttcggca gatgacgcgg cgctttcggt
     3541 gccgaaactc ctttcgccgg gttagctttg agttcgttct ggctgaccag ccagtcaaaa
     3601 aagctacgta gcgcagaaag ccgtaacgcc agacttgctg ctcccagccc tttacggcga
     3661 ctgcgtacag caaaattgcg taccatcgct gcatcgcatt gttgccagct ttgcaggccg
     3721 ttttcgctgg caaaattgat gatcgcctca agctgacgct ggtagttaag tagggttatc
     3781 gggctaagct gacgctccac gctcagataa cgtaggtagc gttctacatc ggtgtgtaaa
     3841 tcggtcatac gcgttcaatc caacgctcca gaagctccgg caacatcagc gcaatttcat
     3901 gaagtaactg cgttccctgc ccttgttgat agtgactggc atcgcgactg gtaaacagca
     3961 caacacccaa atcagcatcg cttcccagca tcgacatcgc caccgatccc accgctttcg
     4021 cttccggtag caccaccagc agctccggtc cgttaagcgg cccaagatag tgctgttcct
     4081 gccccaaacg ctgaatacgc agcggttcga aagactgacg gcttaatgcc agatgagtgt
     4141 ggttcgacgg cgcacctaag cgccagcgat ctggaaacag gcgcagactc gcaccagcta
     4201 ggccgagatc gcgcgcccag cggtgaaagc gcatcagcat atcgtcgaga ctgctggcgg
     4261 cggtgagact gcgctgcagg tagagtaggc gataaaacag cccttcgttg gcgatagcct
     4321 gctccatcaa caacgccatg ttctcttcca gaacatgaat gtgattacgt gcgcgggcca
     4381 tgtgccactc gaccaacgaa acggtgccgc gtaccggatg cggcacacgt atcgcttcta
     4441 ctgcgcgcgc attgcggata aaaaactcag gatttttaat cagataatcg acaaccgccc
     4501 ggtcatcaag ctccgtgagt gtttcctgca gttcttcccc tggttgcttc atagatgaat
     4561 aaatccatcg tagacatgta ccgccgggcc agtcatatat aacgggtgac ccggaccttt
     4621 ccaggcgata tcaagacgac cgccggggag ttccacgcgt acttcttcgt ccagcaaacc
     4681 ttgctgaatc cctaccgcca ccgccgcaca cgcgccgctg ccgcaggcct gggtttctcc
     4741 tgccccacgc tcataaacgc gtaaacgaat atgctcgcgc ttaaccactt gcataaaacc
     4801 gatattggcg cgctccggaa aacgctcgtg actttccaga acaggaccaa gcgtttctac
     4861 cgccgcggta tcgacatcat cgacctgaat cacgcaatgc ggatttccca tcgacaccac
     4921 gccgcataag attgtctgct cggcggcgcg cataatatag gtcttttccg ctttgttagc
     4981 gcgaaacggc acggcggaag gttcgaagtt gggttcgccc atatttacgc ggaccagatc
     5041 atcatcggtg acggtcagaa ccatccgccc gttggcggtg ctgacgcgga tatcacgctt
     5101 attggtcagt cctttcagac gcacaaaacg ggcaaagcag cgcgcaccgt taccgcactg
     5161 cgccacttca ctgccatcag cattgaaaat gcgatagtga aaatccagtt caggatcata
     5221 cggcggctca accaccagca gctggtcaaa ccctaccccc aggtgccgat ccgccaggcg
     5281 acgaatcagc tccggtgaaa aaaagacatt ctgcgttacc gcgtcgacga ccataaaatc
     5341 gttgccaagg ccatgcattt tcgagaactg catcatttac tccaatcacg cgggtacaga
     5401 aactgacttt tagtaattca cctgggatgg accatcgcca gtggcgcgat cgtttttatc
     5461 cggcaccgtg gattgcgttt gcgtctctac cggtttggtc ggcggcggtg cgtttttatc
     5521 tgcaggcggg aaatagagcg gacctttcag accgcagccc gtcaggctga agagagtaag
     5581 taatacagtg agtgccttaa acacgttttt cattatggat tgcctgtaaa gttcatcgct
     5641 ttctgctttc tatcatcgca ggagaaggcg taaaagcaag cgtttagcga ctctttatga
     5701 agtctgaaaa gtgaggaaat gctgcgagta taacgcttac tcgtgaggat ttatgttctg
     5761 ctggcagttt cgtgatcaac acctcgcgcc aggatggctt tatgcactat cgcaatgtgg
     5821 tttgtttgtt gtcttgttca ttgtttttat cgtcggcatg gggatgtcgg ctttccgcac
     5881 ctgaacataa tatttaccag aaacagggaa aaggcgtggt gtacctgcgc ccttataaag
     5941 aaacaaatct ttctctacct gacgtgaatt acaagagttt gcgtcggtta cccaatttat
     6001 taatagaccc gacaacacta gacgaatggg ataaagaacc gcctctgact gatttgacaa
     6061 ccgactacct ctatgagggc gcacaggcct ggtatcctca ttattcatgg catagcgatg
     6121 gtcgcaatat cctctacgcg ggtgaagttg tgcaaaaccc tcccggcaca cctcccgtcg
     6181 atgtcgcctc gttcaaggca tggggtgatt ttgccgcaga taaacacagt ctctattttg
     6241 aaggcaagcg caccgatgac aatggtggtg gaaatagctt ggatatcaaa acgctgcatc
     6301 aggtggaatt ccggcctccc tgggatcctg acttactggg gctgatatta cgtgacgcta
     6361 atttcttgta tatcaatggg catcgcctcg ctgaccccga gagtttccgt gtgctggcgc
     6421 agaagtcctg ggatcagcga ggaaagtttt ccaccacgtt taacccctgc attgccgtac
     6481 catttggccc ctgggatacc ctggctcgta cacggaccaa aatcctgctc aacggcgaac
     6541 agcttgatgc cgacccggac accttttccg tcgtgcgctg gatgcccggc tcactcttga
     6601 cctggcgtga taaaaacggg ctgcagcgta aagtcctcga caaggaaaat ctggcgtggg
     6661 atgaagattt aacaaagcac tgtctggatt tttctctgct ggaaaagaaa gtgttctggc
     6721 gtaaaggacc tgcttgtaaa caggaagaat tacccggact cgatccggag cagtttcaac
     6781 ccatcagtga tgctgtcgcc cagcatcagg actcgcttta taccatcatc gaaacagagt
     6841 ctggtaaccg taagctggag atcgtgaaac ttgatgatcc caatcttatt atcaacaaac
     6901 gtttcaacgc cgggaaacgc cacggctatt tacttacgcg tgccgaaggg tagccatacc
     6961 attccggttt acacgtgttt gaatctgacg gaccgctgat cttactggat aaccgttctc
     7021 cggatgaacg cgaagcccat cttaatgacc atcccttttt gcgcagatgg tatgcccgcg
     7081 ataaccgcta catttacagc tttgatggcg cgcagctctg gcgataccgc accgctgatc
     7141 cgaaacaagt tcgcttaatc tggaaggaac aacattcggg atatgtctat ggcgtaaatt
     7201 acaaaacggg atatctggac ggaaaaatta ctgatgacgg cgaatttatt cctgccccgc
     7261 gcaatgaggc gacaaaatga agggtcaaat aatcacagtt gcactcatcc tgttgggaca
     7321 cacatttctg acgcctgctg tacaggccat agggttcgac tattacaatg atcacggtgt
     7381 tatgtcttac ggtaaaggtt attgggggga taagaaaatc attgcccagt ttccggagat
     7441 gagcagggcc ggtttgcgcc ttgtgaaaaa tatctctggg aaaacgggtt atataccttc
     7501 cgatgaaata agcatagaaa atgaggaata ccactgggtg acagacggcc gcgttattct
     7561 ctggcgtggc aaaatcgtta gcaatccacc gggaacactc actgtcgata ttgccagctt
     7621 tcaggctatg ggccgcttcg cggtcgataa atatagcctc tattttgacg gacagcgcac
     7681 cgaaagtaat agcggcgcgt cacgtgttga gctggcgaca ctaaaagcta tcgaaggtaa
     7741 ctctaccacg ctggtggata gtaaaaatct ctacttgtcc ggtcgacgtc aggggagcag
     7801 cagtgatgtt actgtattag aaaaaagatg gtggggtatt aatccacgtc ttatgagtgt
     7861 aaacagaaat tcatattcca atgatctgct tatccgcagt gggcagaata tttatttaaa
     7921 tggcgttcac cttacggcga atgcagactc atttgagata attcgctgga tacctcattc
     7981 actgctggtt tttcgcgaca ataagggtct gcatcgttat ccctttggtc aattatcagg
     8041 caaagcgata cccgtagatg atgacgtctc ttttgaagta ggggaaagtc gcgttcgctg
     8101 gcgtaaacag ctcacgcccg accgtcagtg gagcaagtgg atagacctac caggtattga
     8161 acctgaac
//
LOCUS       DAJTRC010000144         8103 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.144, whole genome shotgun sequence.
ACCESSION   DAJTRC010000144 DAJTRC010000000
VERSION     DAJTRC010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8103)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8103)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8103
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.144"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            7..657
                     /locus_tag="OO357_003656"
     CDS             7..657
                     /locus_tag="OO357_003656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888464.1"
                     /translation="MHRIDTKTAKKDKFGAGKNGFTRGNPQTGTPATDLDDDYFDMLQ
                     EELCSVVEASGASLEKGRHDQLLTALRALLLSRKNPFGDIKSDGTVKTALENLGLGEA
                     AKRNVGTGANQIPDMSLFASINTVTAAAQKFPSGLILQCGQLNGAPNVSSTYGMRFPM
                     TFSRVIAVVVTLNVTGAAGQPTVSATSVQNTGFNITVSPGSGYGSSADAYYIAMGY"
     gene            629..1063
                     /locus_tag="OO357_003657"
     CDS             629..1063
                     /locus_tag="OO357_003657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5888465.1"
                     /translation="MRITLQWDINKMSYFYSASTNGFYSTEFHGTNIPDDAVEISESE
                     WKTLINAQSVTKMITCGENGHPVIVDRPSPTPEQLALINDEKKSALIAEATNVIAPLQ
                     DAVDLGMATDDETKLLLAWKKYRVLLMRVNVVKPEWPMHPNK"
     gene            complement(1836..2885)
                     /locus_tag="OO357_003658"
     CDS             complement(1836..2885)
                     /locus_tag="OO357_003658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HCR5888466.1"
                     /translation="MYNNKIDSNRKIRHDWVDALKFLGIFAIYLGHLGLGAGKLYPFV
                     FSYHVPLFFFAAGFFTIKKNDLSVFDYIKSKFYRLMIPYFTFAFSILIINTINSGETI
                     DYIYNHIYDIIYGVRNNQFVGTIWFINCLFVIIAIDAIFREIVKNNIVILIISLLSFM
                     LSQTVLNHNPLLDPQWFWNIDSAMAYWWLLPLGRCMFLELTRDRFFGKSKIGFIVFSI
                     TAIMSAYQLLNQKPLLFKIISIFNADIISSSYIQAINTIITTVGLIIFNIFIAKIICG
                     NDFIVRAGRNTLNICGMEYITKLFIPMALAIIGFSVTIPNPICAIIYTCICVYVSDKI
                     GHWLSRTVGGPFLIK"
     gene            3336..4526
                     /locus_tag="OO357_003659"
     CDS             3336..4526
                     /locus_tag="OO357_003659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001111520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl9 family transposase"
                     /protein_id="HCR5888467.1"
                     /translation="MPARKVCQTFFRNALASTHQYRQNAIIDSAAALVGGASLSLTSI
                     GRHLPGPARVKDKIKRVDCLLGNKRLHNDIPLIFKNITSMMTNKLSWCVIAVDWSGYP
                     FQEYHVLRASLLCDGRSIPLMSQVFPSKKNNEAVEIAFLDALSGAISPRTRVVIVTDA
                     GFQSAWFRHIKSQGWDFIGRIRGVVKFRLDSDKDKWLDIKMCRGSSEAKYLGTGTLAR
                     KKRSQCEGHFYLYKHSPKGRKSRRARGRPGLPTTEKEQKAAGREPWLIFSNTAEFNAK
                     KIMKLYSRRMQIEQNFRDEKSERFGFGLRASRSRRGERFLVLSLLVTLASIVLWLLGY
                     HFENKGFHLKYQANSLKKRRVLSFLTLAENVLRFNPELLRRAQPEKILCRLASTYRSM
                     VLAY"
     gene            complement(4916..6376)
                     /gene="gtrII"
                     /locus_tag="OO357_003660"
     CDS             complement(4916..6376)
                     /gene="gtrII"
                     /locus_tag="OO357_003660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase GtrII"
                     /protein_id="HCR5888468.1"
                     /translation="MIKINLFKNANLLAFISCFAISIYCYWGWLYDGTLNIDGEFTNN
                     FYQTITLGRWFHTFLRHYFLPEPFSLYITPLIALSFIIISAFIICRSLKLESYELLIG
                     MLVFITFPQISYQLEFLNQADTVGIAFLLAAISAIIFHSQKNRIVIFSGIVLSILSMA
                     IYQTFVTYIIAFVIGLQINSIIRNEKNIRESFYSSCLSLSLIALSTLIYLLLTKAIKH
                     YFSLESNEYISNYIQNASDIKWLVKSAIDNIYNFYNNPPTGLNLYKWLLIPLLILMFT
                     LTYKLKTRSIYLISSIIFIYILPVIFIVVVGSGAPPRLFVLMPIVAVILFSCLSNFRS
                     IKYLNCMFFLFIIFNGVSTSKNLFLNDTLARQKDISLAKEISYTSQTKGISLNGKYIY
                     IYGSNDSGNMLSMSADTFGKSFFWWDGGNYFRMVAFMNYYGICNCKPANKEQIEKIYP
                     IVKSLPSWPNPDSIAEINGLVIIKLSEKKGWLPFNI"
     gene            complement(6373..7302)
                     /locus_tag="OO357_003661"
     CDS             complement(6373..7302)
                     /locus_tag="OO357_003661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HCR5888469.1"
                     /translation="MKISLVVPVFNEEEAIPVFYKTVREFQELKPYEVEIVFINDGSK
                     DATESIINALAVSDPLVVPLSFTRNFGKEPALFAGLDHASGDAVIPIDVDLQDPIEVI
                     PHLIEKWQAGADMVLAKRSDRSTDGRLKRKTAEWFYKLHNKISTPKIEENVGDFRLMS
                     REVVENIKLLPERNLFMKGILSWVGGPTDVVEYVRAERVAGISKFNGWKLWNLALEGI
                     TSFSTFPLRVWTYIGLFVASISFLYGAWMIIDTLVFGNPVRGYPSLLVSILFLGGVQL
                     IGIGVLGEYIGRIYIEVKNRPKYIIKKSHRGNP"
     gene            complement(7299..7661)
                     /locus_tag="OO357_003662"
     CDS             complement(7299..7661)
                     /locus_tag="OO357_003662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HCR5888470.1"
                     /translation="MLKLFVKYTSIGVLNTLIHWVVFGVCIYAAHTSQALANFTGFVV
                     AVSFSFFANARFTFKALTTAMRYMLYVGFMGILSVIVGWAADKCSLPPIVTLITFSAI
                     SLVCGFVYSKFIVFRDAK"
BASE COUNT         2504 a         1545 c         1640 g         2414 t
ORIGIN      
        1 taatccatgc atcgtataga cacgaaaacc gcgaagaagg ataagttcgg cgcgggtaag
       61 aacggtttta cccgtggtaa cccccagact ggcacgcctg ccaccgatct ggatgatgac
      121 tactttgaca tgttgcagga ggagctttgc agcgtggtgg aggcatccgg tgccagcctg
      181 gagaaggggc ggcacgatca gttgcttacc gcacttcgcg cgctgctgtt aagccgcaag
      241 aatccgtttg gcgatatcaa atcggatggc acggtgaaaa cggctctcga aaaccttggt
      301 ttgggagaag cagctaaaag gaatgtaggt acaggggcga atcagatacc tgatatgagc
      361 ctgttcgcgt caattaatac cgtaacggct gctgcgcaaa aatttccgtc tggattaatt
      421 ttacagtgcg gtcagttgaa tggtgccccg aatgtatctt caacatacgg gatgaggttc
      481 ccgatgacgt tctcaagagt aattgctgtc gtagttacat tgaacgttac tggcgcggct
      541 gggcagccga ctgtatcggc gacaagtgtc cagaacactg gatttaatat tactgtgtcg
      601 cccggttcag gatacggttc atctgctgat gcgtattaca ttgcaatggg atattaacaa
      661 aatgtcatat ttttattctg catcgacaaa cggattttat tcgactgaat ttcacggcac
      721 caatattcct gatgatgcag tggaaatctc ggaatcagag tggaaaacac tgattaatgc
      781 acagagtgta acaaaaatga ttacctgtgg tgagaacggt catcctgtca ttgttgaccg
      841 tccttctcca acaccagaac aattagcctt aataaatgat gaaaagaaat ctgcactgat
      901 agcagaggca acgaatgtaa tagctccgct tcaggatgcg gttgatttag gtatggcaac
      961 agatgatgaa acgaaactgt tactggcatg gaaaaaatat cgggtgctgc ttatgcgtgt
     1021 taatgtagta aaacccgagt ggcctatgca tccaaataaa tagacttgtt aatctaggat
     1081 aattgttgga ataatactcg tttttcagta ggttcaggta gcctgggtat aatgctcact
     1141 tctacaatga tttcaactta ttttatttaa ttgatattac gaaacttaac tcatacataa
     1201 acaatatgac tcaatactgt cctaaatagc tgcgcagaaa agtgctccac tttcgctcag
     1261 cccttgctgg gcgaagcatc aggaaggcgt tctcagctag gttgtgttat atctggcttg
     1321 agttttgttg cccatacgca acacattacc catctgtaaa cgacaagtgt ggcagaatta
     1381 ggtgcagcta tttagactat atcgacgcag taacgataat agatatcgaa actgtactag
     1441 atattagcta cttatggtta ccggggggag tcttttgagc actggtgtta tcaattggct
     1501 tcaaataatt cgccaataaa ttatattgcg atagctcatc accttccagg ttgtttatag
     1561 ccagtttatc agcgtcacca cctactgttt atgcttttat ttctttgatt aataagtata
     1621 tgatatttta attcatctgg tgtatttctt tctcatgtgt tggtcgactg aatatctgtt
     1681 cagtgaaggg gtaattttat gtttgcactt cgaacattcc cttcagttgc taggaaagaa
     1741 aaccgtaaca tttgcgcatt aattttaaca gtataatctg agcataaata tgcgctatat
     1801 atctataaaa aggcgcaatt aattgcgcct ttttatcatt ttatcaaaaa tggcccaccg
     1861 actgtcctcg atagccaatg gccaatttta tctgatacat atacacaaat acaggtatat
     1921 atgattgcgc agataggatt tggaatagtc acgctgaagc ctataattgc taaagccata
     1981 gggataaata atttggtgat atactccatt ccacatatgt taagagtatt tcttccagct
     2041 cttacaataa agtcatttcc acaaattatt tttgctatga aaatattaaa gattatcaag
     2101 cctaccgtgg tgataatagt attaatagct tgaatataac tacttgatat tatgtctgca
     2161 ttgaatattg agataatctt aaaaagtaaa ggtttctggt taagtagttg atacgcactc
     2221 attatagcgg taattgagaa gacaatgaaa ccaattttag attttccaaa aaacctgtca
     2281 cgtgttaatt ctaaaaacat acatctaccg agaggcaata accaccagta tgccatagcg
     2341 gagtctatgt tccaaaacca ttgaggatct agtaatgggt tatgattgag cactgtttgt
     2401 gacaacatga acgataacag tgatatgatt aaaatcacta tattgttctt gactatttct
     2461 ctaaagattg catcaattgc tattataaca aaaaggcagt ttataaacca tattgttcct
     2521 acgaattggt tatttctaac accatatatt atatcataaa tgtggttgta tatataatca
     2581 atcgtctcac ctgagttaat tgtgtttatt attaatatag aaaatgcaaa agtgaaatat
     2641 ggaatcatta atctgtaaaa ctttgacttg atataatcga aaacagataa gtcatttttt
     2701 ttgatagtaa aaaaaccagc agcgaaaaag aatagtggga cgtgataact aaaaacaaaa
     2761 ggatacaatt tgccagcccc caacccaagg tgacctaaat atatagcaaa aataccgaga
     2821 aattttaaag catcaaccca atcatgtctt atttttctgt tgctgtcgat tttgttatta
     2881 tacatttaaa gtaggctctc cagttttgtg tgatttttaa acactcattg caaatatgaa
     2941 tgcctgaaag tttatgatta ttaatcaacg agttcaatat taatttatta tttttcaaag
     3001 tcgcactata taggcattta cttccgcagt accaccgata gtatcgatat caaatcagcc
     3061 tgcggccgcg ataactttcg taacattctg ccagcggctt tgttgaataa atcaggttca
     3121 agataatcgt ctctgcatca ggttagagtt tcaggcaata catcctcttt ctggcatacc
     3181 tgccactctc gcaccatggt catagccttt acgatctgca tactcaccag accagagccc
     3241 gctccctttt aggctgagga atccccagaa aagaagacag gcatagagtt ttgtgatcta
     3301 ctgattgcgc taacaaattc aatgagatca cctctatgcc tgcccgtaaa gtatgccaga
     3361 cttttttccg taatgcttta gcttccacgc atcagtatcg acaaaatgcc attattgatt
     3421 ctgctgctgc tttagtcggt ggcgcatctc tttctcttac cagcattggc cggcatttac
     3481 ccggtcctgc tcgcgtgaaa gataaaataa aacgcgttga ctgtctgttg ggtaataaac
     3541 gacttcataa tgatatccct ctgatattca aaaatattac atccatgatg acaaataaac
     3601 tgtcgtggtg tgttatcgct gttgactgga gcggttaccc ttttcaggaa tatcatgtcc
     3661 ttcgtgccag cctcctctgc gacggtcgct ctatccccct gatgagtcag gtcttcccgt
     3721 caaaaaaaaa taacgaggcg gtggaaatcg cttttctgga tgcgctttcc ggggccattt
     3781 ctccccggac tcgtgttgtt attgttactg atgctggttt tcaaagtgca tggttccgtc
     3841 atatcaaatc acagggatgg gattttattg gtcgtatcag gggggtagtg aagttcaggc
     3901 tggatagtga caaagataag tggctggaca taaaaatgtg cagggggagt tcagaggcaa
     3961 aatacctggg gacgggaacc ctggcccgga aaaaacgctc gcagtgtgaa ggacattttt
     4021 atctttataa acattcacct aaaggcagaa aaagcaggcg tgccagaggg aggccgggct
     4081 taccaacaac ggaaaaagaa caaaaagccg caggcagaga accatggtta atattcagca
     4141 atacagctga gtttaatgcc aaaaaaatca tgaaactgta cagtcgaaga atgcagatag
     4201 agcaaaactt tcgggatgaa aaaagtgaac gctttggttt tggccttcgg gcgagcagaa
     4261 gtcggagggg agagcgtttt ctggtattaa gtctactggt tacactggcc agtatcgtac
     4321 tctggctact tggttatcat tttgaaaata aaggatttca tttaaagtat caggctaata
     4381 gtctcaaaaa acgaagagta ttgtcttttc tgacgctggc tgaaaacgtc ctgagattta
     4441 accctgaact tctacgacga gcacaacctg aaaagatatt gtgccggttg gccagcactt
     4501 accgaagtat ggtgctggct tactgatgaa aattttctgg ggatccctca gcctttcggg
     4561 gaggaatgag ggctctgata ttcttttgcc gtagttcatc gtgaaaccgt cggatgtcat
     4621 ttggcctgag tgacccatgc ctcccaaaag cagagtcgct agtgttatgt aattaggcgg
     4681 ttaaacttca attctcagtg ttaagtttgg gtaaacgctt gtaaatggta ttgctaattt
     4741 tttaattgac taatattaga aacggttagt gtgattgtat atagatattt gagtgtaagg
     4801 tatttaaagg ggcattcagc ccctccgtct atttgtgatt gcatccattg atgtgtgtat
     4861 tattttgagc ttgaaaaagg gaggcgctct ttaataataa ataaatctat caaagctaaa
     4921 tattaaatgg aagccaccct tttttctctg atagttttat tatcaccaaa ccatttattt
     4981 cagctatcga atctggattc ggccaggaag gtaaactctt cacaattgga taaatcttct
     5041 ctatttgttc tttatttgct ggtttgcaat tacagattcc atagtaattc ataaatgcaa
     5101 ccatcctaaa atagttgcca ccatcccacc aaaaaaaaga ttttccaaaa gtgtctgcgc
     5161 tcatggagag catatttcct gagtcgtttg aaccatatat atatatatat tttccgttaa
     5221 gggaaatgcc ttttgtttga gatgtgtatg atatttcttt agctaaagag atatctttct
     5281 gtcttgcgag agtatcattc aaaaatagat ttttggatgt tgaaacgcca ttaaatataa
     5341 taaataaaaa aaacatgcag tttaggtatt ttatagagcg aaaattgctt aagcaagaaa
     5401 acaaaattac tgctactata ggcattaaaa caaacaggcg aggtggcgcc cctgagccaa
     5461 caacaacgat aaatataacc ggaagtatat aaatgaaaat gattgatgaa atcaaataaa
     5521 ttgatcttgt ttttaattta tatgttaggg taaacatcag aattaataaa ggaatcagta
     5581 accacttgta taggtttaaa ccagtgggag gattgttata gaagttatat atattatcta
     5641 tggctgattt aacaagccat ttaatatcgc ttgcattttg tatataattt gagatgtact
     5701 cgttcgattc aagcgaaaaa taatgcttga tagctttggt taatagcagg taaattaagg
     5761 tagataaagc tatgagggat agagataaac atgaactata aaaagattca cgaatatttt
     5821 tctcatttcg tattatcgaa tttatctgca acccaatgac gaatgcaata atatatgtta
     5881 cgaatgtttg gtagattgcc attgaaagaa tggacagtac tataccagaa aatatcacaa
     5941 tcctattttt ttgcgagtga aaaataattg ctgatatcgc tgccagtaga aaagcaattc
     6001 ccacagtatc agcttggtta agaaactcta attgatagga gatctgaggg aaggtaataa
     6061 atactaacat acctatcaat aattcataag attctagctt tagcgatctg cagattataa
     6121 atgctgaaat gatgataaaa gataaggcta ttaatggtgt tatataaagt gaaaaaggct
     6181 cagggagaaa gtaatgtcgc aaaaaagtgt gaaaccaccg cccaagcgtg attgtttgat
     6241 aaaaattatt tgtaaactcg ccatcaatat taagtgttcc atcgtacaac cacccccaat
     6301 aacaataaat tgatattgca aaacatgata tgaaggcaag gagatttgca tttttaaata
     6361 aattaatttt aatcatgggt tacctcgatg agattttttt atgatgtatt tgggtctatt
     6421 ttttacttca atatagattc tacctatata ttctccgaga acaccaatcc cgatcagttg
     6481 cactccaccc aagaaaagta ttgatacaag cagggaggga tacccgcgta ctgggttacc
     6541 aaagacaagg gtgtctataa tcatccatgc accatataaa aatgaaatgc ttgcaacaaa
     6601 caagcctata taagtccata cgcgaagagg aaaggttgaa aaacttgtga taccttccag
     6661 tgccagattc cataatttcc agccattaaa ttttgagatg ccagcaacac gctctgcgcg
     6721 tacatattca acgacatccg tcggaccacc cacccagctc agtatgcctt tcatgaaaag
     6781 attgcgctca ggcaacagtt taatgttctc cacaacctca cgagacatga gtcgaaaatc
     6841 tccgacattt tcctcgatct ttggggtgct tattttgttg tgtaatttat agaaccactc
     6901 agctgtctta cgtttcagtc gtccatcagt tgagcggtct gaacgtttag caagcaccat
     6961 gtcagcacct gcctgccact tttcaataag atgagggata acctcaattg ggtcttgcag
     7021 gtcgacatca ataggaatta cagcatcgcc gcttgcatgg tctaaccctg caaataaggc
     7081 tggttcttta ccaaagttgc gtgtaaatga cagcggaaca actagcgggt ctgaaacagc
     7141 cagcgcgtta ataattgact ctgtggcatc tttacttccg tcatttatga atacaatttc
     7201 tacttcatat ggcttcaact cttggaattc acgtaccgtt ttatagaaaa caggtatcgc
     7261 ttcttcttca ttgaagacag gaacgacaag agatattttc atttcgcatc cctaaagaca
     7321 atgaactttg aatagacgaa accgcacacc agactgatgg cggagaaggt gataagagtg
     7381 actattggag gaagtgagca tttatcagct gcccaaccaa caatcacact caatattccc
     7441 ataaatccca cgtataacat gtagcgcatc gctgtagtca atgctttgaa tgtgaatctt
     7501 gcattcgcga agaagctaaa gctcacagcc actacgaaac ctgtgaagtt tgccagagcc
     7561 tgactggtat gtgcggcata gatacataca ccaaaaacca cccagtgtat aagtgtgttc
     7621 agcacaccta tagatgtgta ctttacaaat aactttaaca tttatttaat caatgagctc
     7681 tgaaaggcat gaagtctatc atccaagtct caattgatcg atacttgcta tgtctgatga
     7741 gacaaaactg agacacataa ggcctcacaa tggcttgcaa ggctttacat gttttgatgt
     7801 ggtgggacgt gtgagcgcag tgttgatggg ataatccttt gaattacaag cggattctta
     7861 taattcgtaa tgcgaaggtc gtaggttcga ctcctattat cggcaccatt taaatcaata
     7921 agttacctcg catttaagta aactacgttc tcctcttgtg ccgtatttgt gccattgcga
     7981 cttataatcg catcgatttt gctcgcgtgc tcggtaagat gcccggctga aaggtgggcg
     8041 tatctttgaa ccatttcgag agtttcccat cctcccatct ctttaagcgc aagaagagag
     8101 aca
//
LOCUS       DAJTRC010000145         8019 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.145, whole genome shotgun sequence.
ACCESSION   DAJTRC010000145 DAJTRC010000000
VERSION     DAJTRC010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 8019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.145"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..100
                     /locus_tag="OO357_003663"
     CDS             <1..100
                     /locus_tag="OO357_003663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR5888471.1"
                     /translation="LKILIAKGANQCARAGDKLPDLLDEYIRTTTE"
     gene            72..1982
                     /locus_tag="OO357_003664"
     CDS             72..1982
                     /locus_tag="OO357_003664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase large subunit family protein"
                     /protein_id="HCR5888472.1"
                     /translation="MNISEQQLSNMMVAVTIALQPLVRVLPVTAVEWADQNYYLPKES
                     SYGEGEWKTLQFQVAIMNCMGNDEIRTVNLIKSARVGYTKMVLGVIGYFIEHKSRNTL
                     LFQPTDSAAEDFMKSHVEATIRDVPCLKKLSPWLGRKHRDNTLTLKRFSSGVGFWCLG
                     GAAAKNYREKSVDVVCYDELSSFEPDVEKEGSPTLLGDKRIEGSVWPKSIRGSTPKIK
                     GSCQIEKAANESAHFMRFYVPCPHCGEEQYLKFGDDATSFGLKWEKGKPETVYYLCEH
                     NGCVIRQSELDQTGGRWICDNTGMWTCDGLTFFSASGNEIPPPRSITFHIWTAYSPFT
                     TRVQIVCDWLDALKDPNGVKTFVNTTLGETWEEAVGEKLDHQVLMDKVVPYTATVPVR
                     VVYLTAGIDSQRNRFEMYVWGWAPGEEAFLVDKIIIMGRPDEEETLLRVDAAINKKYR
                     HADGTEMTISRVCWDIGGIDGEIVYQRSKKHGIFRVLPVKGASVYGKPVITMPKTRNQ
                     RGVYLCEVGTDTAKEILYARMKADPTPVNEATSYAIRFPDNPEIFSQTEAQQLVAEEL
                     VEKWEKGKMRLLWDNKKRRNEALDCLVYAYAALRVSVQRWQLDLAVQLHPARMQLHNR
                     PLKNWQRSCPEE"
     gene            1983..2186
                     /locus_tag="OO357_003665"
     CDS             1983..2186
                     /locus_tag="OO357_003665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5888473.1"
                     /translation="MVTVAELQALRQARLDLLTGKRVVSVQKDGRRIEYTAASLNELN
                     RAINDAESVLGTTRRRRPLGVRL"
     gene            2183..3776
                     /locus_tag="OO357_003666"
                     /pseudo
     CDS             2183..3776
                     /locus_tag="OO357_003666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309134.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
     gene            3766..5271
                     /locus_tag="OO357_003667"
     CDS             3766..5271
                     /locus_tag="OO357_003667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S49 family peptidase"
                     /protein_id="HCR5888474.1"
                     /translation="MRRNLSHIIAAAFNEPLLLEPAYARVFFCALGREMGAASLSVPQ
                     QQVQLDAPGMLAETDEYMAGGKRPARVYRVVNGIAVLPVTGTLVHRLGGMRPFSGMTG
                     YDGIVACLQQAMADTQVRGVLLDIDSSGGQATGAFDCADMIYRLRQLKPVWALCNDTA
                     CSAAMLLASACSRRLVTQTSRIGSIGVMMGHVSYAGHLAQAGVDITLIYAGSHKVDGN
                     QFESLPAKVRQDMQQRIDAARRMFAEKVAMYTGLSVDAVMGTEAAVFEGQSGIEAELA
                     DELINASDAISVMAATLNTHDTGGTMPQLTATEAAAQENQRVMGILTCQEAKGREQLA
                     TMLAGQQGMSVEQARAILAVAAPQQSGVSAQSEADRIMACEEAKGREQLAATLAAMPE
                     MTVEKARPILAASPQANAGPSLRDQIMALDEAKGAEAQAEKLAACPGMTVENARAVLA
                     AASGRAEPVSASTTAMFEHFMANHSPAAVQGGVPQTSADGDADVKMLMAMP"
     gene            5308..5655
                     /locus_tag="OO357_003668"
     CDS             5308..5655
                     /locus_tag="OO357_003668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head decoration protein"
                     /protein_id="HCR5888475.1"
                     /translation="MVTKTITEQRAEVRIFAGNDPAHTATGCSGISSATPALTPLMLD
                     EDTGKLVVWDGQKAGHVVGILVLPLEGTETVLTYYKSGTFATEAIHWPERVDTHKKAN
                     AFAGSALSHAALP"
     gene            5713..6741
                     /locus_tag="OO357_003669"
     CDS             5713..6741
                     /locus_tag="OO357_003669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="major capsid protein"
                     /protein_id="HCR5888476.1"
                     /translation="MGLFTTRQLLGYTEQKVKFRALFLELFFRRTVNFHTEEVMLDKI
                     TGKTPVAAYVSPVVEGKVLRHRGGETRVLRPGYVKPKHEFNYQQAVERLPGEDPAQLN
                     GPAYRRLRIITDNLKQEEHAIVQVEEMQAVNAVLYGKYTMEGEQFETVEVDFGRSVGN
                     NITQAGGTEWSAQDRDTFDPTHDLDAYCDFASGTINIAIMDGKVWRLLNGFKLFREKL
                     DTRRGSNSQLETAVKDLGAVVSFKGYYGDLAIVVAKTSYVAEDGTEKRYLPDGTLVLG
                     NTAAEGIRCYGAIQDAQALSEGVVASSRYPKHWLTVGDPAREFTMTQSAPLMVLPDPD
                     EFVVVQVK"
     gene            6793..7179
                     /locus_tag="OO357_003670"
     CDS             6793..7179
                     /locus_tag="OO357_003670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5888477.1"
                     /translation="MATKEENLKRLCELAERLGREPDVSGSAADIAQRVAELEEELGD
                     AGEVAEPDTYSPSKDDPTAHKGESVPEQPGIVTGDETGQVTVVALATLHTKCNGSVIF
                     VSPGTSFRVSAEVAASMAAGGLAKRQ"
     gene            7191..7568
                     /locus_tag="OO357_003671"
     CDS             7191..7568
                     /locus_tag="OO357_003671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5888478.1"
                     /translation="MDGFDNLFDAALAGVNEVILRDMGISAVITSGELEGTHLTGVFD
                     DPESISFVAGGIRLEDSSPCLFVKTADISQLRRQDTLTIGDDSFFVDRITPDDGGCCY
                     IRLRRGSPAPQNNARMRRYDEGT"
     gene            7555..>8019
                     /locus_tag="OO357_003672"
     CDS             7555..>8019
                     /locus_tag="OO357_003672"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706636.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5888479.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQTSAWAVNRVAAKIVSVATRQVAQ
                     NTVAGDNQVKGIPLKTVRERVRLLKASPSGKMYARMRVNRGNLPAIKLGTAQVRLARS
                     RHGSNSRHRGSVLKVGKYLFRDAFIQQLANGRWHVMRRIDGKNRYPIDVVKIP"
BASE COUNT         1852 a         1883 c         2496 g         1788 t
ORIGIN      
        1 tctgaaaatc cttattgcta agggagcgaa tcagtgcgcg cgtgcggggg ataaattacc
       61 ggatttactc gatgaatata tcagaacaac aactgagtaa tatgatggtg gcagtcacta
      121 tcgcactgca gccactggtc agggtattgc cggtgacggc tgtagaatgg gccgatcaaa
      181 attattacct gcctaaagaa tcgtcatacg gtgaggggga atggaaaacg ctgcagtttc
      241 aggtcgccat catgaactgc atgggcaatg acgagattcg tacagttaac ctgattaaat
      301 ctgcccgtgt tggttataca aaaatggtgc ttggtgtgat cgggtatttt attgagcaca
      361 aatcccgcaa cactcttctt tttcagccga cggactcagc tgctgaagat tttatgaagt
      421 ctcacgtgga agcaacaatt cgtgatgttc cttgcctgaa aaaactttct ccctggctgg
      481 gccggaaaca ccgggataat acgctcacac tgaagcgttt ttcctctggt gtggggttct
      541 ggtgtttggg tggtgctgct gccaaaaact accgtgaaaa atctgtcgat gtggtctgct
      601 atgacgaact ctcctcgttt gaaccggatg tggaaaaaga aggttcgcca acactgcttg
      661 gcgataaacg tattgaaggt tcggtatggc caaaatccat acgcggttca acgccaaaaa
      721 ttaaaggctc ctgtcagatt gaaaaagctg cgaatgaatc tgcgcatttc atgcggtttt
      781 atgtcccttg ccctcattgc ggggaggagc agtatctgaa gtttggcgat gatgcgacgt
      841 cgtttggcct gaaatgggag aagggtaaac cggaaacggt gtattacctg tgtgaacata
      901 atggctgcgt gatccgtcag tctgaacttg atcagaccgg ggggcgctgg atttgtgaca
      961 ataccgggat gtggacgtgt gacggtctga cgtttttcag tgcttcgggt aatgaaattc
     1021 cgccgccgcg ctccatcact ttccatatat ggacggcgta cagtccgttc accaccaggg
     1081 tacagatagt ctgtgactgg ctggatgcac tgaaagatcc caacggcgtg aaaacttttg
     1141 tgaacaccac gctgggcgag acctgggaag aggccgtggg cgaaaaactc gatcaccagg
     1201 tactgatgga taaggttgtg ccttacacgg caacggtgcc tgtccgggtg gtttatctga
     1261 cggcgggcat tgactcgcag cgaaaccgtt ttgagatgta tgtctggggg tgggctccgg
     1321 gagaggaagc ctttctggtg gataaaatca tcatcatggg acgtcctgat gaggaagaga
     1381 cgctgttacg tgtggatgcg gcgatcaata aaaaataccg ccatgcagac ggtaccgaaa
     1441 tgaccatttc ccgtgtctgc tgggatatcg gggggatcga tggcgaaatc gtgtatcaga
     1501 ggtcaaaaaa acacggtatt ttccgggtac tgccggtaaa aggtgcctct gtctatggca
     1561 agccggtgat caccatgccg aaaacccgca atcagcgggg tgtgtatctg tgtgaggtgg
     1621 ggacggatac cgcaaaagaa attctctatg cccgaatgaa ggccgatccc acccctgtga
     1681 atgaggccac atcgtatgcc atccgctttc ctgataatcc ggagattttt tcgcagacag
     1741 aggcacaaca actggtggcg gaggaactgg tggaaaagtg ggaaaaagga aagatgcgtc
     1801 tgttgtggga taacaaaaag cggcgtaacg aagcgctgga ctgcctggtg tatgcctatg
     1861 cggcattacg tgtgtccgtg caacgctggc aacttgatct ggctgtgcag ttgcatcccg
     1921 caaggatgca actgcacaac cgacccttga agaactggca gcgaagctgt ccggaggagt
     1981 gaatggttac agtcgctgaa ctgcaggcgc tgcgtcaggc gcgccttgat ttattaaccg
     2041 gtaaacgggt tgtgtcggta cagaaagacg gtcgcaggat cgaatatacg gcagcgtccc
     2101 tgaatgagct taaccgtgcg atcaacgatg cggagtcggt tctgggtacg acccgacgcc
     2161 gccgaccgct gggagtgagg ttatgaaacg aacgcctgtc ctgattgatg tgaacggcgt
     2221 tccgcttcgt gagagtctca gctacaacgg aggcggcgca ggatttggcg ggcaaatggc
     2281 agagtggtta ccaccggcgc agagtgccga tgcggccctg ttgcctgcat tgcgtctggg
     2341 gaatgcccgg gcagatgatc tggtgcgcaa taacggtatc gcggctaatg cggtggccct
     2401 gcataaggat catatcgtcg ggcacatgtt tcttatcagc taccgtctga actggcgttg
     2461 gctggggatg cgggagacca cggcaaaaag ttttgtcggg gaggtggaag cagcctggtc
     2521 ggaatacgca gaaggaatgt ttggcgagat cgacgtggaa gggaaacgca cgtttacgga
     2581 atttatccgt gaaggggtgg gcgtgcatgc ctttaacggc gaaatctttg tgcagccggt
     2641 ctgggatacg gaaaccacgc agttattccg tacgcgtttt aaagccgtga gtccgaaacg
     2701 ggtggacacg ccaggacacg gtatggggaa ccactttctg cgggccgggg ttgaggtcga
     2761 tcggtatggg cgggcgattg cgtaccatat ctgtgaggat gattttccgt tctctggtgc
     2821 cggacgctgg gagcggatcc cgcgtgagct tcccaccggg cgtccggcca tgctgcatat
     2881 ttttgagccg gtggaggacg ggcagacccg tggggctaat cagttttaca gcgtaatgga
     2941 gcggatgaag atgctggact ccctgcaggc cacacagctt cagtcggcca ttgtgaaggc
     3001 gatgtatgca gccacaatcg aaagtgaact tgataccgaa aaggcatttg aatatatcgc
     3061 gggggcaccg caaggtcagc aggataatcc gcttattaat attctggaga agttcacccg
     3121 ctggtatgac acgaataacg tgacgctggg cggtgttaaa attccgcacc ttttttcctg
     3181 gcgatgcgct gaacctgcag acagcgcagg attcagacaa tggattttcg gcgcttgaac
     3241 aggcgctgct gcggtatatt gcagccggtc ttggcgtttc ctacgaacag ttgtcccgtg
     3301 attactcgaa ggtcagttac tcaagtgccc gcgcctccgc caatgagtcg tggcgctatt
     3361 ttatggggcg gcgaaaattt attgcggccc ggctggccac gcagatgttt tcctgctggc
     3421 tggaagaggc gcttcttcgg gggattattc gtccgccacg ggcgcgtttt gatttttacc
     3481 aggcgcgttc agcctggtca cgggcagagt ggattagctc cggaagaatg gccattgacg
     3541 ggctcaagga ggtccaggaa tcggtgatgc gcattgaggc cggactgagc acgtatgaga
     3601 aagaactggc actgatgggc gaagattatc aggacatttt ccgccagcag gtcagggaat
     3661 ccgcagagcg cgaaaaagcc ggactctcac gtccggtgtg gatagcccag gcgtatcagc
     3721 agcagatagc ggagagtcgc aggccggaag aggagacaac atcacgtgag acgtaatctt
     3781 tcacatatta tcgctgcggc attcaatgag ccgctgcttc tggagcccgc ctatgcgcgg
     3841 gttttctttt gcgcgctcgg gcgtgagatg ggcgcagcaa gcctttcggt accgcaacag
     3901 caggtacagc ttgacgctcc cgggatgctg gctgaaacgg acgagtacat ggccggaggc
     3961 aaacgacctg cccgtgttta ccgggtggtg aacggtattg ctgtactgcc ggtgaccggc
     4021 acgctggtac accggctggg tggcatgcgg ccattttccg gaatgactgg ctatgacggc
     4081 attgtcgcct gtcttcagca ggcaatggcg gacactcagg tacggggcgt actgctggac
     4141 attgacagtt ctggcgggca ggccaccggc gcgtttgact gcgctgacat gatttaccgt
     4201 ctccggcagc tgaagcctgt ctgggcactg tgcaatgaca cggcctgttc agccgccatg
     4261 ttactggcat cggcctgctc ccgacggctg gttacccaga catcccgtat cggctccatt
     4321 ggggtgatga tgggccatgt cagctatgcc ggtcatctgg cacaggccgg tgtggatatc
     4381 acgctgattt acgccggatc gcacaaggtg gatggcaatc agtttgaatc cctgccagca
     4441 aaggttcgcc aggacatgca gcagcgcatt gatgcggcgc gccggatgtt tgctgaaaaa
     4501 gtggcgatgt ataccggttt atctgtggat gcggttatgg gaacagaggc cgccgttttt
     4561 gaaggtcagt ccggtattga ggccgaactg gcggatgaat taatcaatgc gtcggacgcc
     4621 atcagcgtga tggctgcgac gctgaacaca cacgatacag gaggcactat gccgcaatta
     4681 actgcaacgg aggctgccgc gcaggagaac cagcgagtga tggggatcct gacgtgtcag
     4741 gaagcgaaag gacgtgaaca actggccacg atgctggcag ggcaacaggg tatgagcgtt
     4801 gagcaggccc gggcgattct ggccgtggcg gcaccgcaac aatctggtgt atctgcgcag
     4861 agtgaagccg atcgcattat ggcatgtgaa gaggcgaaag gacgtgaaca actggcggca
     4921 acgctggcgg cgatgccgga gatgacggtg gaaaaagccc gtccgattct ggctgcttca
     4981 ccgcaggcga atgccggacc ctcacttcgt gatcagatta tggccctgga tgaggcaaaa
     5041 ggggcagagg cgcaggctga aaaactggcg gcctgtccgg ggatgaccgt ggagaatgcc
     5101 cgggctgtac tggcggcagc ctcaggcagg gcagaaccgg tttctgcatc cacaaccgcc
     5161 atgtttgaac atttcatggc gaaccattca ccggcagcgg tacagggtgg cgtgccacag
     5221 acgtcagcag acggtgatgc ggacgtgaaa atgctcatgg ccatgccatg aagtcagtgc
     5281 tgaccatcaa tatgaggttt ttacaatatg gtaacgaaaa ccatcactga acagcgtgcg
     5341 gaagtacgta tttttgccgg taatgatccg gcccataccg ccacaggctg cagcgggatt
     5401 tcctcggcaa caccggctct gacgcccctg atgctggatg aggataccgg gaaactggtg
     5461 gtctgggacg gacagaaggc aggccatgtg gttggcatac tggtactgcc gcttgaaggt
     5521 acagagacgg tgctgacgta ttacaagtcg gggacctttg cgacggaggc catccactgg
     5581 cctgagagag tggatacaca caaaaaggcg aatgcctttg ccggcagtgc cctgagtcac
     5641 gcagcgctgc cgtaacattt tatcaggtca ctgcggtagc ctgactgatt tctgaatgaa
     5701 aggaactgat ttatgggatt gtttacgacc cgccagttac tcggttatac cgaacaaaaa
     5761 gttaaatttc gtgcgctgtt tctggagctg tttttccgcc gtacggtgaa tttccatacc
     5821 gaagaggtaa tgctggacaa aattaccgga aaaacgccgg tggcggccta tgtctccccg
     5881 gttgttgaag gaaaggtgtt gcgtcaccgt ggcggtgaaa ctcgtgtact gcgcccgggc
     5941 tacgttaagc cgaaacacga atttaattac cagcaggcgg tggagcgtct tcccggtgaa
     6001 gatccggccc agctgaacgg cccggcttac cgtcgcctgc gtatcatcac cgataacctc
     6061 aaacaggaag agcacgccat tgtccaggtg gaagagatgc aggcggtgaa tgccgtgctg
     6121 tatggcaaat acaccatgga aggagagcag tttgaaactg ttgaggtcga ttttggccgg
     6181 tcggtgggaa ataacattac gcaggcagga gggacggagt ggagcgctca ggaccgcgat
     6241 acctttgatc cgacgcatga tctggatgcg tactgtgatt ttgcttccgg caccatcaat
     6301 attgccatta tggatggtaa ggtctggcgt ctgctgaatg gctttaagct gttccgcgaa
     6361 aaactggata cccgtcgtgg ctcaaattca cagctggaaa cggcagtgaa agacctgggg
     6421 gcggtggtgt ccttcaaagg gtattatggt gatctggcaa tcgtggtggc aaaaacgtcc
     6481 tatgtggcag aggacggtac cgaaaaacgt tatctgccgg acggcacact ggtactggga
     6541 aatacggccg cagagggtat tcgttgctac ggtgccattc aggatgcgca ggcattgtct
     6601 gaaggcgtgg tggcgtcttc ccgttatccg aaacactggc tgaccgtggg tgatccggcg
     6661 cgtgaattca ccatgacgca gtccgcgccg ctgatggtgc tgccggatcc ggatgagttt
     6721 gtggtggtgc aggtgaaata atccgtgagc gggagctaaa tgctcccgtt tttcattcaa
     6781 tcaggagcgg gtatggctac gaaagaagaa aacctgaaac gtctttgtga gctggctgag
     6841 aggctggggc gtgaaccgga tgtctccggt agtgcggcag atattgcgca gcgagtggcg
     6901 gaactggagg aagagcttgg agatgcgggg gaggtcgcag agccggatac gtactcacca
     6961 tcgaaagatg atccgaccgc gcataaaggc gagtcagttc ctgaacagcc aggcattgtt
     7021 accggagacg aaacggggca ggttacggtg gtggcactgg cgacactgca tacaaaatgt
     7081 aatggttcag tgatatttgt ttcaccggga acgtcgtttc gtgtttctgc tgaggtggct
     7141 gccagcatgg cggcaggtgg tctggcaaaa aggcagtaac aggagatgtc gtggacggtt
     7201 tcgataatct gtttgatgct gcgcttgccg gtgtcaatga agtcattctc cgtgatatgg
     7261 ggatcagcgc cgtcattacg tccggagaac ttgaggggac acacctgaca ggggtttttg
     7321 atgatcccga aagtatttct tttgttgccg gggggatccg gcttgaggat tcctcaccgt
     7381 gcctgtttgt gaaaacggca gatatatcgc agttacgccg tcaggatacg ttaactatcg
     7441 gggatgattc tttctttgtg gatcgtatta ctccggatga cgggggatgc tgttatatcc
     7501 ggttaaggcg gggcagtcct gccccacaga ataatgccag aatgaggcga tatgatgaag
     7561 ggacttgaga atgccatccg caatctgaac agcctcgata cccgcatggt gccacagacc
     7621 agcgcatggg cggtaaaccg tgtggcggca aagattgttt ctgtcgccac acggcaggtt
     7681 gcgcagaata ccgttgccgg ggataaccag gtgaaaggta ttcccctgaa aacggtcagg
     7741 gagcgtgtca ggctgcttaa agccagcccc tcaggaaaaa tgtatgcccg catgcgtgtt
     7801 aaccggggta acctgcccgc cattaagctg ggtaccgcac aggtcagact ggcccgctcc
     7861 cggcatggca gcaactcacg tcatcgtggc agcgtactga aagtggggaa ataccttttc
     7921 cgggatgcgt ttattcagca actggcgaat ggtcgctggc atgtgatgcg gcgtattgac
     7981 ggcaaaaatc gttaccccat tgatgtggtg aaaatcccg
//
LOCUS       DAJTRC010000146         7940 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.146, whole genome shotgun sequence.
ACCESSION   DAJTRC010000146 DAJTRC010000000
VERSION     DAJTRC010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7940
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.146"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(14..707)
                     /gene="ospZ"
                     /locus_tag="OO357_003673"
                     /pseudo
     CDS             complement(14..707)
                     /gene="ospZ"
                     /locus_tag="OO357_003673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000625270.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector cysteine
                     methyltransferase OspZ"
     gene            763..915
                     /locus_tag="OO357_003674"
                     /pseudo
     CDS             763..915
                     /locus_tag="OO357_003674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            2296..3243
                     /gene="icsP"
                     /locus_tag="OO357_003675"
     CDS             2296..3243
                     /gene="icsP"
                     /locus_tag="OO357_003675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858404.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="omptin family outer membrane protease IcsP"
                     /protein_id="HCR5888480.1"
                     /translation="MKLKFFVLALCVPAIFTTHATTNYPLFIPDNISTDISLGSLSGK
                     TKERVYHPKEGGRKISQLDWKYSNATIVRGGIDWKLIPKVSFGVSGWTTLGNQKASMV
                     DKDWNNSNTPQVWTDQSWHPNTHLRDANEFELNLKGWLLNNLDYRLGLIAGYQESRYS
                     FNAMGGSYIYSENGGSRNKKGAHPSGERTIGYKQLFKIPYIGLTANYRHENFEFGAEL
                     KYSGWVLSSDTDKHYQTETIFKDEIKNQNYCSVAANIGYYVTPSAKFYIEGSRNYISN
                     KKGDTSLYEQSTNISGTIKNSASIEYIGFLTSAGIKYIF"
     gene            complement(3805..4177)
                     /locus_tag="OO357_003676"
                     /pseudo
     CDS             complement(3805..4177)
                     /locus_tag="OO357_003676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611434.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing N-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            4421..4540
                     /locus_tag="OO357_003677"
                     /pseudo
     CDS             4421..4540
                     /locus_tag="OO357_003677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309413.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
     gene            4790..5656
                     /gene="ospB"
                     /locus_tag="OO357_003678"
     CDS             4790..5656
                     /gene="ospB"
                     /locus_tag="OO357_003678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspB"
                     /protein_id="HCR5888481.1"
                     /translation="MNLDGVRPYCRIVNKKNESISDIAFAHIIKRVKNSSCTHPKAAL
                     VFLGEKGFCDSNDVLSIMGQQIPRVFKNKMLYDYVFKNEKSKNDFLKMAESWLPQSEP
                     IVINNDDDALNAAAYFSVKKAKIKTVNDTDFKEYNKVYILGHGSPGSHQLGLGSELID
                     VQTIISRMKDCGILNVKDIRFTSCGSADKVAPKNFNNAPAESLSCILNSLPFFKEKES
                     LLEQIKKHLENDESLSDGLKISGYHGYGVHYGQELFPYSHYRSTSIPADPEHTVKRSS
                     QKKTFIINKELD"
     gene            5985..6725
                     /gene="phoN2"
                     /locus_tag="OO357_003679"
     CDS             5985..6725
                     /gene="phoN2"
                     /locus_tag="OO357_003679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAP2 family phosphatase PhoN2"
                     /protein_id="HCR5888482.1"
                     /translation="MKTKNFLLFCIATNMIFIPSANALKAEGFLTQQTSPDSLSILPP
                     PPAENSVVFQADKAHYEFGRSLRDANRVRLASEDAYYENFGLAFSDAYGMDISRENTP
                     ILYQLLTQVLQDSHDYAVRNAKEYYKRVRPFVIYKDATCTPDKDEKMAITGSYPSGHA
                     SFGWAVALILAEINPQRKAEILRRGYEFGESRVICGAHWQSDVEAGRLMGASVVAVLH
                     NTPEFTKSLSEAKKEFEELNTPTNELTP"
     gene            complement(7085..>7940)
                     /gene="ospC4"
                     /locus_tag="OO357_003680"
     CDS             complement(7085..>7940)
                     /gene="ospC4"
                     /locus_tag="OO357_003680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_156647404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="type 3 secretion system effector OspC4"
                     /protein_id="HCR5888483.1"
                     /translation="KLPLNKTHHTVDFGANAYIIDHDSPYGYMTLTDHFDNAIPPVFY
                     HEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIFNTKDMRLGIGLHLIDFIRKSKDQG
                     FREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLSTDNFKKIKLRDISLEDAIKASNYE
                     EINNKVTDKKMAHQALAYSLGNKKADIALYLLSKFNFTKQDVAEMEKMKNNRYCNLYD
                     VEYLLSKDGANYKVLEYFINNGLVDVNKKFQKANSGDTMLDNAMKSKDSKMIDFFIKK
                     WSGIRQTI"
BASE COUNT         2488 a         1487 c         1488 g         2477 t
ORIGIN      
        1 caattaatat acattaagta acaggcattc gagccttgat ttgagaaagc ctgtcattca
       61 tttgttttat gtagctatct atttcctctg atgcataact aaaaagttct cccatttcac
      121 gagcaataat aatctcagat ttaatagact ttaatctctg gcgactcatc tcttaacact
      181 tgcataatct ctctttttat atcatatagt tctataaacc gatttgcccg tagttctcct
      241 tgggaagaat catttctgaa tacaacaatt atatacaaaa aatcactatt ttctttagag
      301 aagatcttac aactaatctc tgggagctca cataatgcat gtaagtcagg atggtacaaa
      361 ctatccacag aaaattgccc cataggacgt tttccagata taactgtcaa ccagcttcct
      421 tccggtgtac tggcttcggc tctggaaata ggctgaaaat cttccggaca tggagaatac
      481 attgtcttca tataattaag tcgtctggat ttctcattaa taaactcctc tatttttaat
      541 tgtgccatac ttctggttat accagagata tacttcttgt catatatgtt tggtgattca
      601 ttaaagtaac catgttcaac tctaggataa atatttctca ctatatattc agtatttgca
      661 gtatttattg gaaatacgtt cttaatattc ttgatgggac taatcatttt aatctctata
      721 ctcaaatata aacattacca tgaacaatat aattgttcct aagtggaatg tctccacgga
      781 gaacacttta tctatcggga aatagtgcgg gcaacagtgt ttaattatat cgagtgcaat
      841 tacaatcgct ggcgacggca ccgttggtgt ggcggcctca gtccggaaca atttgaaaac
      901 cagaacctcg cttaggcctg tgtccatatt acgtgggtag gctcacagag cctcacttac
      961 gactgcgtcc atattatgtg ggtaggatca ataaagacgt tttatatgtg tctgcttgca
     1021 gccagaaaat ttataccctg caggtatcat aaacatgctt gataacttaa ttggggctcc
     1081 ctcttccttt tatggtgtaa ttgtttgatt gaatacttcc ggggatttca gtatgaaatg
     1141 aagtatattt aatatacttt acaaaaagtt tgtattagtg gaataaggac aatagcttat
     1201 ccaaactgcc aagcctcttt attataagta agatctggca ctggaatatt ctcaaaagga
     1261 ctaacatcat caccacgacc ttcaaccaac cattttattg catttaaatc ccagaatagt
     1321 tcagtagtgg tcaatttggg aaacgcacaa tattgtacgg ttacgttatc gcaatagagt
     1381 gaactctcgc cctatacggc gttttcaagg attagtcctt aatcggacaa ccacattaaa
     1441 gcataataaa taattaagct ttaataatat actatagata cagttctatt ataaaaaata
     1501 ctattttagg tgatgcatta aattagtaga atatttattc ttttataaaa gcaccaatga
     1561 attgtccaag taaacatgag ataaaatgtg tagaggataa ctgagtttgg tcactttaac
     1621 tgtattagtc gcagtctgac tctcgacttt aaaaggatgg gaattacctg tactgatagt
     1681 gagaaagctt tccattgaaa taagtcaaat aaaagttgat ggcttcagtg ctaaagtgag
     1741 gtgcccgcta gccgtattca ctcgaatcgg aattcaatgt attgcttctg ccatttccat
     1801 aatatttcac gggaaaaaca aaatctgaaa aactatactt aagtgttgta ttgagtcagt
     1861 tgagatacaa atacatattt atcagcacac gttacgtcaa atacaggagg ttgtcatgtt
     1921 ggtactgaaa ggcacgttgg ctattgcagc cgtgatgaca atttattgtc ttatcgttgt
     1981 tcttatggaa cgcttttctg attgatttca tattggcgag agaacaagaa ggtgagtaga
     2041 attgtcgcgg aatcctgaaa ctatcagcct caggtatgaa caccaaaagg taggtagaga
     2101 aatctatggc ccccgttact ttattcgcac aatgaggtat ttttttattt ttacaaaagt
     2161 tcaataaata ttataataaa agatatgttc ttggtaacat aaacaaatat ttaattaaaa
     2221 taatgcttgg cattgacgtt tatcttataa agtaagaaga tcatggacat ttcaaccaaa
     2281 aaagtagagt tctcgatgaa attaaaattc tttgtacttg cactttgtgt acctgcgatc
     2341 tttactacac atgctaccac taactatcca cttttcatac cggacaacat cagtacggat
     2401 attagtctag gatctctgag tggcaaaaca aaagaacgcg tttatcatcc caaggaaggg
     2461 gggagaaaaa ttagtcaact ggactggaaa tacagtaatg cgactattgt tagaggtggc
     2521 atcgattgga agctaattcc aaaagtgtct ttcggagttt ccggttggac tactttaggt
     2581 aaccagaaag caagcatggt tgataaagac tggaacaatt ccaatacccc tcaggtatgg
     2641 accgaccaga gctggcatcc caatacgcat ctccgtgatg ctaacgaatt cgagctgaat
     2701 cttaaaggtt ggttattaaa taatttggat tatcgacttg gactaatagc aggttaccaa
     2761 gagagtcgtt acagttttaa tgcaatggga gggagttata tttatagtga aaacgggggg
     2821 agcaggaata aaaaaggggc acatcctagt ggtgaaagaa caataggtta caaacagctc
     2881 ttcaaaatac cttatattgg attaactgct aattaccgcc atgagaattt tgagtttgga
     2941 gcagaactga aatatagtgg ttgggttctt tcatctgata cagataaaca ctatcagact
     3001 gagacaattt ttaaagatga aataaaaaac caaaattact gctctgttgc tgcgaatatt
     3061 ggatactatg tcacccccag tgcaaaattt tatatagaag gctccagaaa ttacatttct
     3121 aataaaaaag gtgatacatc tctttatgag caaagtacca atatatctgg caccattaaa
     3181 aatagtgcaa gtattgaata tattggtttt ctcacttccg caggtataaa gtatattttt
     3241 tgaattggtt caccgagttg attacgtttt catggctaaa aattgccgcc cattgtgagt
     3301 catattcgcc ctgactttcc agaaccatac gaactgcccg ttgacggact tcgggggaaa
     3361 aacgtgtatt tttagtcatt ctgtttacct ctttctcagg gcgtttagtc tccaggattt
     3421 ccggggcggt tcaaaaaact cttcgttgat tactgtggac ttactgttgg cattaccgac
     3481 cctgagaccg gagaaataag aaccgcttag atcatcgtgg ctgttctcgg ggtatcaagt
     3541 tacacatggg cagaggctac ccaaggtcta ctattatcgc ctgaaagcgc tgatggaaca
     3601 gtgctatcag ggacatgctg atgaaaatac ctaaataatc agccagcact ctatctttcc
     3661 aaatccacag catagcaaag agagcaaaag agcctgtaaa ttcagaaatt aaggaaacgc
     3721 ctcttggtgt acataatcta tcttatgtta aatgaaatct aatttcttga ttatatgctg
     3781 gatcgttcgt gggtggcttt gcccctatat ttccagacac ctgttatcat tggcccgctg
     3841 ccgcagatat tcccgtggcg agcgataatc cagcgcacta tgcggatgcc attcgttata
     3901 atgctcgaat gcctctgcaa ggttctttgc tgccgttaac ccgtcaggtt tgggcatgat
     3961 actgatgtag tcacacttta ttgttttcac gaagctcttt gctattctgt tactctccgg
     4021 actccgcacc gccgtgttct tcggttcaac aaagtcccaa catccgggtg aactggcgtg
     4081 tttcattagc tcggtagcat gaaccattat ccgtcagtca cttcactgga gacgccggaa
     4141 gctcgttgcc gaagcggtgt tccaccgctc ccagcatgtc gccaacggga gagcatctac
     4201 ggtctgattg aactgtttat tcaaattgtc caccgtctgt cagtacgctc tgaacgtcgg
     4261 ctggttaaaa cactgctcgc tgattttcag agggttcacg gtaaaacagc acttttattc
     4321 cggattgcgg aagcggctct gaataatccg gatgggctgg ttaaagaggt tgtgtaccat
     4381 ctgcatattg ttgaaccaga tcgcagcggt aagagaagtt catcatactt ggcgcagtta
     4441 cgcgatgttt ctgctagagg tgatgccgta aagaacggca gaacattacc ggaacaagat
     4501 agcggtctac ctgcactggt atcagacccg gggcttcccc ggatgatctc cacggtactt
     4561 taggtactgt gtctatatta tgtaggtagg atcaattaac tttgtagttg aaattctttc
     4621 attgaaataa tgaataaaaa agacaaaccg cattaaacaa tgccagaata taattgcatt
     4681 tttgttccgt ttttttaaat catagtatac aaagaagctt gcatactata tgaaaatata
     4741 acacccttta ctatgatgtt tataaaacaa tatatggagt catgtaggta tgaatttaga
     4801 tggtgttaga ccatactgta gaatagtcaa taaaaagaat gaaagcatat cagatattgc
     4861 atttgcacat ataataaaaa gggtaaaaaa ttcatcatgt actcacccaa aagcagcatt
     4921 ggttttttta ggagagaaag gtttttgtga tagcaatgat gttctatcta ttatgggaca
     4981 acaaatacca agagtattta agaacaagat gttatatgat tatgttttta aaaatgaaaa
     5041 aagtaaaaat gattttctaa aaatggctga atcatggcta ccacagagtg aaccaatagt
     5101 aataaataat gatgatgacg cattgaatgc tgctgcttat ttttctgtaa aaaaagcgaa
     5161 aataaaaaca gtaaacgata ctgattttaa agagtataat aaggtttata ttcttgggca
     5221 cggtagtcct ggttctcatc aattaggcct tggttcggaa cttattgatg tacaaacaat
     5281 catttcaaga atgaaagact gtggtattct aaatgtgaaa gatatccgtt ttacttcatg
     5341 cggctccgct gataaagtgg ctcctaaaaa ttttaacaat gcccctgctg aaagtctttc
     5401 ttgtatcctt aactctctgc ctttttttaa ggaaaaagaa tctttgctag agcagataaa
     5461 aaaacacctt gaaaacgatg agtcattgag tgatggtcta aaaatatccg gctatcatgg
     5521 atatggagtt cactatggtc aagagctttt tccctactca cattatcgtt caacttcaat
     5581 tcctgctgat ccggagcata cagtaaaaag aagctctcag aaaaagactt ttattattaa
     5641 taaagaactg gattagtata aaatttttaa cctatagtta attgtttgca tttaagatga
     5701 agttatttta tctttagtag aaaagtgctt ccctatgagg gaaaatcaca aatcatcata
     5761 atacagagac aaaacgatac gaaaaaatag ataaaaaaca tcattctttt accacattat
     5821 tttccgtgaa tatgaaaaat aatgttatta ctttaatata agactatttt ttgtttttcc
     5881 atcactctgt tcaaattttt ccgcatgact tgtgtttttt gtaatacagc tcgtttttta
     5941 cagctgacca aaatcatcaa ttaattatgc taaggaaata aattatgaaa accaaaaact
     6001 ttcttctttt ttgtattgct acaaatatga tttttatccc ctcagcaaat gctctgaagg
     6061 cagaaggttt tctcactcaa caaacttcac cagacagttt gtcaatactt ccgccgcctc
     6121 cggcagagaa ttcagtagta tttcaggctg acaaagctca ttatgaattc ggccgctcgc
     6181 tccgggatgc taatcgtgta cgtctcgcta gcgaagatgc atactacgag aattttggtc
     6241 ttgcattttc agatgcttat ggcatggata tttcaaggga aaatacccca atcttatatc
     6301 agttgttaac acaagtacta caggatagcc atgattacgc cgtgcgtaac gccaaagaat
     6361 attataaaag agttcgtcca ttcgttattt ataaagacgc aacctgtaca cctgataaag
     6421 atgagaaaat ggctatcact ggctcttatc cctctggtca tgcatccttt ggttgggcag
     6481 tagcactgat acttgcggag attaatcctc aacgtaaagc ggaaatactt cgacgtggat
     6541 atgagtttgg agaaagtcgg gtcatctgcg gtgcgcattg gcaaagcgat gtagaggctg
     6601 ggcgtttaat gggagcatcg gttgttgcag tacttcataa tacacctgaa tttaccaaaa
     6661 gccttagcga agccaaaaaa gagtttgaag aattaaatac tcctaccaat gaactgaccc
     6721 cataaagctg gacagcctgt atcaggctat ggagggccca tagacaaatc taccctatat
     6781 gagcatagga ggagtctatg ggcacaccac gttttacccc tgaatttaag ggattactgg
     6841 aaaggctggg acatatccct ccggcagaag cagaaaaagc ttattatgct gccatcggaa
     6901 acgatgatct ggcaacctga gttcacagat aaaacattct ctaggaaact cggggcggtt
     6961 ccgttcacca catgcaatgt ggtgttgcag gggaacggtc tgcaccatcc cctatgtcga
     7021 tcaatataac agaaatgaca acttcagatt cagggcacaa cctaaatata ttttaggtca
     7081 cctctcaaat cgtttgcctg ataccgctcc attttttaat aaaaaaatca atcatttttg
     7141 aatctttgct tttcattgca ttgtcaagca tagtgtcccc actatttgct ttttgaaatt
     7201 ttttgtttac atcaaccaat ccattattga taaaatattc tagcacttta tagttggcac
     7261 catctttact aagtaagtat tcaacatcat acagattaca atatctattg tttttcattt
     7321 tctccatctc tgcgacatct tgttttgtaa aattaaattt agatagtaaa taaagtgcta
     7381 tatcagcttt tttattgcct aaagaataag caagggcctg atgagccatt tttttatcag
     7441 tcactttatt gttaatctct tcataattag atgctttaat cgcatcctcc aaagatatgt
     7501 ctctgagttt tatttttttg aagttatctg tacttagcat tcttggtata tgatactcaa
     7561 gctgaaacac aaagtttata attctatcca gactaacagg atcaatattt ttgttataac
     7621 aaaactctct gaatccttga tctttactct ttcttataaa atcaataaga tgcaacccaa
     7681 ttccaagcct catatcttta gtgttaaata ttggaacatc agttttcccc tgattaccat
     7741 gcacatacct gctaacttca tccacaacct ctttaaagtt atctaagaaa aatgattggt
     7801 gctcatggta aaaaacaggt ggaatagcat tatcaaagtg atcggttaat gtcatatatc
     7861 catatggaga gtcatgatca atgatatacg cattggcgcc aaaatctaca gtatgatgtg
     7921 ttttatttag tgggagtttt
//
LOCUS       DAJTRC010000147         7909 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.147, whole genome shotgun sequence.
ACCESSION   DAJTRC010000147 DAJTRC010000000
VERSION     DAJTRC010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.147"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(123..953)
                     /locus_tag="OO357_003681"
     CDS             complement(123..953)
                     /locus_tag="OO357_003681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5888484.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
     gene            1163..2176
                     /gene="intI1"
                     /locus_tag="OO357_003682"
     CDS             1163..2176
                     /gene="intI1"
                     /locus_tag="OO357_003682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5888485.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            2379..2729
                     /locus_tag="OO357_003683"
     CDS             2379..2729
                     /locus_tag="OO357_003683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5888486.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            2855..3415
                     /locus_tag="OO357_003684"
     CDS             2855..3415
                     /locus_tag="OO357_003684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5888487.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            3418..6384
                     /locus_tag="OO357_003685"
     CDS             3418..6384
                     /locus_tag="OO357_003685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5888488.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     TLVALATEGMATVTDEIIDLHDRILGKLFNAAKNKHQQQFQASGKAINAKVRLYGRIG
                     QALIDAKQSGRDAFAAIEAVMSWDSFAESVTEAQKLAQPDDFDFLHRIGESYATLRRY
                     APEFLAVLKLRAAPAAKNVLDAIEVLRGMNTDNARKLPADAPTGFIKPRWQKLVMTDA
                     GIDRRYYELCALSELKNSLRSGDIWVQGSRQFKDFEDYLVPPEKFTSLKQSSELPLAV
                     ATDCEQYLHERLTLLEAQLATVNRMAAANDLPDAIITESGLKITPLDAAVPDTAQALI
                     DQTAMVLPHVKITELLLEVDEWTGFTRHFTHLKSGDLAKDKNLLLTTILADAINLGLT
                     KMAESCPGTTYAKLAWLQAWHTRDETYSTALAELVNAQFRHPFAGHWGDGTTSSSDGQ
                     NFRTASKAKSTGHINPKYGSSPGRTFYTHISDQYAPFHTKVVNVGLRDSTYVLDGLLY
                     HESDLRIEEHYTDTAGFTDHVFALMHLLGFRFAPRIRDLGDTKLYIPKGDAAYDALKP
                     MIGGTLNIKHVRAHWDEILRLATSIKQGTVTASLMLRKLGSYPRQNGLAVALRELGRI
                     ERTLFILDWLQSVELRRRVHAGLNKGEARNALARAVFFNRLGEIRDRSFEQQRYRASG
                     LNLVTAAIVLWNTVYLERAAHALRGNGHAVDDSLLQYLSPLGWEHINLTGDYLWRSSA
                     KIGAGKFRPLRPLQPA"
     gene            6451..6828
                     /locus_tag="OO357_003686"
     CDS             6451..6828
                     /locus_tag="OO357_003686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5888489.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(7029..7688)
                     /locus_tag="OO357_003687"
     CDS             complement(7029..7688)
                     /locus_tag="OO357_003687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5888490.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
BASE COUNT         1753 a         2241 c         2133 g         1782 t
ORIGIN      
        1 cttccctcat gatgtttaac gtttgaaata aggggcgccg agcgccagcg aggggagcca
       61 aaagcttgct tttggccgtc ccgacttgat tgaagggttg ggcgattttg ccattagatt
      121 ttttataaat ttagtgtgtt tagaatggtg atcgcatttt tcttggcttt tatgcttgat
      181 gttaaattcg accccaagtt tcctgtaagt gcggacacaa aaacatattt atgtcctgat
      241 ttgcttataa taaacccttc aaaccatccg ttttgtaagg ttctatttgc tgtgaatcct
      301 gcaccagttt tcccatacag ttttgtacta ttatccagat cttgtagata catgttctct
      361 atggtgtttt ctatggctga gtttttaact gggagattgt gattaataat tttacgcagg
      421 aattgaattt gttcttctgg tgaaattttt aagctacttt cgagccatgc ttctgttaat
      481 ccgttgtttc tttctttatc tccagagaag tcttgatttc cataatcaaa atctttgaga
      541 taattcttga ttttatttaa tccaattttt tgggttattt cttgcgaaac ccaaacaaca
      601 gaaaattgca tccacgtctt tggtgtatga ttgctgttcc agatctccat tcctttgggg
      661 gttttatccc atttgaatat ggttttctga tctattattt ccgcatcaaa tgccataagt
      721 gataatgcga tcttgaaagt tgaatctggt gccatttgcg ttgcacactt tgctttattg
      781 aattgagcaa tttcagcgtt tgtggatgca tcgtaaagta aaaaacaacc ttcagttcct
      841 tcaaataatg gagatgcaac agtagagata tctgttgatg cactggcgct gctgtagata
      901 atatttgcaa ttattaaaaa aatagcgaag ttgatatgta ttgtgttttt cataataagt
      961 attggtttgg taaagggctt aattttaacg gctaacaatt aatgaggctc cgggttcgcc
     1021 caactttgtt ttagggcgac tgccctgctg cgtaacatcg ttgctgctcc ataacatcaa
     1081 acatcgaccc acggcgtaac gcgcttgctg cttggatgcc cgaggcatag actgtacccc
     1141 aaaaaaacag tcataacaag ccatgaaaac cgccactgcg ccgttaccac cgctgcgttc
     1201 ggtcaaggtt ctggaccagt tgcgtgagcg catacgctac ttgcattaca gcttacgaac
     1261 cgaacaggct tatgtccact gggttcgtgc cttcatccgt ttccacggtg tgcgtcaccc
     1321 ggcaaccttg ggcagcagcg aagtcgaggc atttctgtcc tggctggcga acgagcgcaa
     1381 ggtttcggtc tccacgcatc gtcaggcatt ggcggccttg ctgttcttct acggcaaggt
     1441 gctgtgcacg gatctgccct ggcttcagga gatcggaaga cctcggccgt cgcggcgctt
     1501 gccggtggtg ctgaccccgg atgaagtggt tcgcatcctc ggttttctgg aaggcgagca
     1561 tcgtttgttc gcccagcttc tgtatggaac gggcatgcgg atcagtgagg gtttgcaact
     1621 gcgggtcaag gatctggatt tcgatcacgg cacgatcatc gtgcgggagg gcaagggctc
     1681 caaggatcgg gccttgatgt tacccgagag cttggcaccc agcctgcgcg agcagctgtc
     1741 gcgtgcacgg gcatggtggc tgaaggacca ggccgagggc cgcagcggcg ttgcgcttcc
     1801 cgacgccctt gagcggaagt atccgcgcgc cgggcattcc tggccgtggt tctgggtttt
     1861 tgcgcagcac acgcattcga ccgatccacg gagcggtgtc gtgcgtcgcc atcacatgta
     1921 tgaccagacc tttcagcgcg ccttcaaacg tgccgtagaa caagcaggca tcacgaagcc
     1981 cgccacaccg cacaccctcc gccactcgtt cgcgacggcc ttgctccgca gcggttacga
     2041 cattcgaacc gtgcaggatc tgctcggcca ttccgacgtc tctacgacga tgatttacac
     2101 gcatgtgctg aaagttggcg gtgccggagt gcgctcaccg cttgatgcgc tgccgcccct
     2161 cactagtgag aggtagggca gcgcaagtca atcctggcgg attcactacc cctgcgcgaa
     2221 ggccatcggt gccgcatcga acggccggtt gcggaaagtc ctccctgcgt ccgctgatgg
     2281 ccggcagcag cccgtcgttg cctgatggat ccaacccctc cgctgctata gtgcagtcgg
     2341 cttctgacgt tcagtgcagc cgtcttctga aaacgacaat ggaggtggta gccgagggtg
     2401 tggaaacacc cgactgcctt gcgtggttgc ggcaggcggg ttgcgacacg gtgcagggtt
     2461 tcctgttcgc caggccgatg ccggcggcgg ccttcgtcgg cttcgtcaac caatggagga
     2521 acaccaccat gaacgccaat gaaccgagca ccagttgctg cgtgtgctgc aaggaaatcc
     2581 cgctcgatgc cgccttcacg ccggaagggg ccgagtacgt ggagcatttc tgcgggctgg
     2641 agtgctatca gcgcttccag gcgcgggcca gcactgcgac cgaaaccagc gtcaaaccgg
     2701 acgcttgtga ttcgccgccg tcaggttgag gcatacccta acctgatgtc agatgccatg
     2761 tgtaaattgc gtcaggatag gattgaattt tgaatttatt gacatatctc gttgaaggtc
     2821 atagagtctt ccctgacatt ttgcagggaa ttccatgact ggacagcgca ttgggtatat
     2881 cagggtcagc accttcgacc agaacccgga acggcaactg gaaggcgtca aggttgatcg
     2941 cgcttttagc gacaaggcat ccggcaagga tgtcaagcgt ccgcaactgg aagcgctgat
     3001 aagcttcgcc cgcaccggcg acaccgtggt ggtgcatagc atggatcgcc tggcgcgcaa
     3061 tctcgatgat ttgcgccgga tcgtgcaaac gctgacacaa cgcggcgtgc atatcgaatt
     3121 cgtcaaggaa cacctcagtt ttactggcga agactctccg atggcgaacc tgatgctctc
     3181 ggtgatgggc gcgttcgccg agttcgagcg cgccctgatc cgcgagcgtc agcgcgaggg
     3241 tattgcgctc gccaagcaac gcggggctta ccgtggcagg aagaaatccc tgtcgtctga
     3301 gcgtattgcc gaactgcgcc aacgtgtcga ggctggcgag caaaagacca agcttgctcg
     3361 tgaattcgga atcagtcgcg aaaccctgta tcaatacttg agaacggatc agtaaatatg
     3421 ccacgtcgtt ccatcctgtc cgccgccgag cgggaaagcc tgctggcgtt gccggactcc
     3481 aaggacgacc tgatccgaca ttacacattc aacgataccg acctctcgat catccgacag
     3541 cggcgcgggc cagccaatcg gctgggcttc gcggtgcagc tctgttacct gcgctttccc
     3601 ggcgtcatcc tgggcgtcga tgaactaccg ttcccgccct tgttgaagct ggtcgccgac
     3661 cagctcaagg tcggcgtcga aagctggaac gagtacggcc agcgggagca gacccggcgc
     3721 gagcacctga gcgagctgca aaccgtgttc ggtttccggc ccttcaccat gagccattac
     3781 cggcaggccg tccagatgct gaccgagctg gcgatgcaaa ccgacaaagg catcgtgctg
     3841 gccagcgcct tgatcgggca cctgcggcgg cagtcggtca ttctgcccgc cctcaacgcc
     3901 gtcgagcggg cgagtgccga ggcgatcacc cgtgctaacc ggcgcatcta cgacgccttg
     3961 gccgaaccac tggcggacgc gcatcgccgc cgcctcgacg atctgctcaa gcgccgggac
     4021 aacggcaaga cgacctggtt ggcttggttg cgccagtctc cggccaagcc aaattcgcgg
     4081 catatgctgg aacacatcga acgcctcaag gcatggcagg cactcgatct gcctaccggc
     4141 atcgagcggc tggttcacca gaaccgcctg ctcaagattg cccgcgaggg cggccagatg
     4201 acacccgccg acctggccaa attcgagccg caacggcgct acgccactct cgtggcgctg
     4261 gccaccgagg gcatggccac cgtcaccgac gaaatcatcg acctgcacga ccgcatcctg
     4321 ggtaagctgt ttaacgctgc caagaataag catcagcagc agttccaggc gtcaggcaag
     4381 gccatcaacg ccaaggtacg tctgtacggg cgcatcggtc aggcgctgat cgacgccaag
     4441 caatcaggcc gcgatgcgtt tgccgccatc gaggccgtca tgtcctggga ttcctttgcc
     4501 gagagcgtca ccgaggcgca gaagctcgcg caacccgatg acttcgattt cctgcatcgc
     4561 atcggcgaga gctacgccac cctgcgccgc tatgcaccgg aattccttgc cgtgctcaag
     4621 ctgcgggccg cgcccgccgc caaaaacgtg cttgatgcca ttgaggtgct gcgcggcatg
     4681 aacaccgaca acgcccgcaa gctgccagcc gatgcaccga ccggcttcat caagccgcgc
     4741 tggcagaaac tggtgatgac cgacgccggc atcgaccggc gctactacga actgtgcgcg
     4801 ctgtccgagt tgaagaactc cctgcgctcg ggcgacatct gggtgcaggg ttcacgccag
     4861 ttcaaggact tcgaggacta cctggtaccg cccgagaagt tcaccagcct caagcagtcc
     4921 agcgaattgc cgctggccgt ggccaccgac tgcgaacaat atctgcatga gcggctgacg
     4981 ctgctggaag cacaacttgc caccgtcaac cgcatggcgg cagccaacga cctgccggat
     5041 gccatcatca ccgagtcggg cttgaagatc acgccgctgg atgcggcggt gcccgacacc
     5101 gcgcaggcgc tgatagacca gacagccatg gtcctgccgc acgtcaagat caccgaactg
     5161 ctgctcgaag tcgatgagtg gacgggcttc acccggcact tcacgcactt gaaatcgggc
     5221 gatctggcca aggacaagaa cctgttgttg accacgatcc tggccgacgc gatcaacctg
     5281 ggcctgacca agatggccga gtcctgcccc ggcacgacct acgcgaagct cgcttggctg
     5341 caagcctggc atacccgcga cgaaacgtac tcgacagcgt tggctgaact ggtcaacgct
     5401 cagtttcggc atccctttgc cgggcactgg ggcgatggca ccacatcatc atcggacgga
     5461 cagaatttcc gaaccgctag caaggcaaag agcacggggc acatcaaccc aaaatatggc
     5521 agcagcccag gacggacttt ctacacccac atctccgacc aatacgcgcc attccacacc
     5581 aaggtggtca atgtcggcct gcgcgactca acctacgtgc tcgacggcct gctgtaccac
     5641 gaatccgacc tgcggatcga ggagcactac accgacacgg cgggcttcac cgatcacgtc
     5701 ttcgccctga tgcacctctt gggcttccgc ttcgcgccgc gcatccgcga cctgggcgac
     5761 accaagctct acatcccgaa gggcgatgcc gcctatgacg cgctcaagcc gatgatcggc
     5821 ggcacgctca acatcaagca cgtccgcgcc cattgggacg aaatcctgcg gctggccacc
     5881 tcgatcaagc agggcacggt gacggcctcg ctgatgctca ggaaactcgg cagctacccg
     5941 cgccagaacg gcttggccgt cgcgctgcgc gagttgggcc gcatcgagcg cacgctgttc
     6001 atcctcgact ggctgcaaag cgtcgagcta cgccgccgcg tgcatgccgg gctgaacaag
     6061 ggcgaggcgc gcaatgcgct ggcccgtgcc gtgttcttca accgccttgg tgaaatccgt
     6121 gaccgcagtt tcgagcagca gcgctaccgg gccagcggcc tcaacctggt gacggcggcc
     6181 atcgtgctgt ggaacacggt ctacctggag cgtgcggcgc atgcgttgcg cggcaatggt
     6241 catgccgtcg atgactcgct attgcagtac ctgtcgccac tcggctggga gcacatcaac
     6301 ctgaccggtg attacctatg gcgcagcagc gccaagatcg gcgcggggaa gttcaggccg
     6361 ctacggcctc tgcaaccggc ttagcgtgct ttattttccg ttttctgaga cgacccctat
     6421 tatggcgaag gtatcattca ggaagcgttg atgaaggatt atctctgtaa aaagttattc
     6481 aaccggcttt ccggtaccct ggtgatcaga gcgcgctgcg gcaataacat tactggcctg
     6541 gcatgttgca atattcttta tccctcgccc cgatacagcg gtcagctgca tattaaagag
     6601 ctgtatgttt ctcagtgcga tcgaaataag ggcacgggta aagcgataat gcgctttata
     6661 gcccggcttg cgcttgaaca ggaatgcctt agccttagct ggaacgctga aaaatccaac
     6721 cccggcgcta accgttttta tcaggctctg ggaggcagaa taaatgatca tatcgtcaat
     6781 tattacctcc acggggagag cctgagcaaa ctggcctcag gcatttgaga agcacacggt
     6841 cacactgctt ccggtagtca ataaaccggt aaaccagcaa tagacataag cggctattta
     6901 acgaccctgc cctgaaccga cgaccgggtc gaatttgctt tcgaatttct gccattcatc
     6961 cgcttattat cacttattca ggcgtagcaa ccaggcgttt aagggcacca ataactgcct
     7021 taaaaaaatt acgccccgcc ctgccactca tcgcagtact gttgtaattc attaagcatt
     7081 ctgccgacat ggaagccatc acaaacggca tgatgaacct gaatcgccag cggcatcagc
     7141 accttgtcgc cttgcgtata atatttgccc atggtgaaaa cgggggcgaa gaagttgtcc
     7201 atattggcca cgtttaaatc aaaactggtg aaactcaccc agggattggc tgagacgaaa
     7261 aacatattct caataaaccc tttagggaaa taggccaggt tttcaccgta acacgccaca
     7321 tcttgcgaat atatgtgtag aaactgccgg aaatcgtcgt ggtattcact ccagagcgat
     7381 gaaaacgttt cagtttgctc atggaaaacg gtgtaacaag ggtgaacact atcccatatc
     7441 accagctcac cgtctttcat tgccatacgg aattccggat gagcattcat caggcgggca
     7501 agaatgtgaa taaaggccgg ataaaacttg tgcttatttt tctttacggt ctttaaaaag
     7561 gccgtaatat ccagctgaac ggtctggtta taggtacatt gagcaactga ctgaaatgcc
     7621 tcaaaatgtt ctttacgatg ccattgggat atatcaacgg tggtatatcc agtgattttt
     7681 ttctccattt tagcttcctt agctcctgaa aatctcgata actcaaaaaa tacgcccggt
     7741 agtgatctta tttcattatg gtgaaagttg gaacctctta cgtgccgatc aacgtctcat
     7801 tttcgccaaa agttggccca gggcttcccg gtatcaacag ggacaccagg atttatttat
     7861 tctgcgaagt gatcttccgt cacaggtatt tattcggcgc aaagtgcgt
//
LOCUS       DAJTRC010000148         7892 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.148, whole genome shotgun sequence.
ACCESSION   DAJTRC010000148 DAJTRC010000000
VERSION     DAJTRC010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7892)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7892)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7892
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.148"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..106)
                     /locus_tag="OO357_003688"
     CDS             complement(<1..106)
                     /locus_tag="OO357_003688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin"
                     /protein_id="HCR5888491.1"
                     /translation="MKLLKVAAIAAIVFSGSALAGVVPQYGGGGNHGGG"
     gene            complement(147..602)
                     /gene="csgB"
                     /locus_tag="OO357_003689"
     CDS             complement(147..602)
                     /gene="csgB"
                     /locus_tag="OO357_003689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curlin minor subunit CsgB"
                     /protein_id="HCR5888492.1"
                     /translation="MKNKLLFMMLTILGAPGIAAAAGYDLANSEYNFAVNELSKSSFN
                     QAAIIGQAGTNNSAQLRQGGSKLLAVVAQEGSSNRAKIDQTGDYNLAYIDQAGSANDA
                     SISQGAYGNTAMIIQKGSGNKANITQYGTQKTAVVVQRQSQMAIRVTQR"
     gene            1356..2006
                     /gene="csgD"
                     /locus_tag="OO357_003690"
     CDS             1356..2006
                     /gene="csgD"
                     /locus_tag="OO357_003690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator CsgD"
                     /protein_id="HCR5888493.1"
                     /translation="MFNEVHSIHGHTLLLITKPSLQATALLQHLKQSLAITGKLHNIQ
                     RSLDDISSGSIILLDMMEADKKLIHYWQDTLSRKNNNIKILLLNTPEDYPYRDIENWP
                     HINGVFYAMEDQERVVNGLQGVLRGECYFTQKLASYLITHSGNYRYNSTESALLTHRE
                     KEILNKLRIGASNNEIARSLFISENTVKTHLYNLFKKIAVKNRTQAVSWANDNLRR"
     gene            2011..2400
                     /gene="csgE"
                     /locus_tag="OO357_003691"
     CDS             2011..2400
                     /gene="csgE"
                     /locus_tag="OO357_003691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgE"
                     /protein_id="HCR5888494.1"
                     /translation="MKRYLRWIVAAEFLFAAGNLHAVEVEVPGLLTDHTVSSIGHDFY
                     RAFSDKWESDYTGNLTINERPSARWGSWITITVNQDVIFQTFLFPLKRDSEKTVVFAL
                     IQTEEALNRRQINQALLSTDDLAHDEF"
     gene            2425..2841
                     /gene="csgF"
                     /locus_tag="OO357_003692"
     CDS             2425..2841
                     /gene="csgF"
                     /locus_tag="OO357_003692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgF"
                     /protein_id="HCR5888495.1"
                     /translation="MRVKHAVVLLMLISPLSWAGTMTFQFRNPNFGGNPNNGAFLLNS
                     AQAQNSYKDPSYNDDFGIETPSALDNFTQAIQSQILGGLLSNINTGKPGRMVTNDYIV
                     DIANRDGQLQLNVTDRKTGQTSTIQVSGLQNNSTDF"
     gene            2868..3574
                     /locus_tag="OO357_003693"
                     /pseudo
     CDS             2868..3574
                     /locus_tag="OO357_003693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001571243.1"
                     /note="involved in the stability of the curlin proteins
                     during assembly; involved in the secretion of the major
                     curlin subunit CsgA across the outer membrane;
                     frameshifted; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="curli production assembly/transport protein
                     CsgG"
     gene            complement(3707..4198)
                     /locus_tag="OO357_003694"
     CDS             complement(3707..4198)
                     /locus_tag="OO357_003694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1097 domain-containing protein"
                     /protein_id="HCR5888496.1"
                     /translation="MNILLSIAITTGILSGIWGWVAVSLGLLSWAGFLGCTAYFACPQ
                     GGLKGLAISAATLLSGVVWAMVIIYGSALAPHLEILGYVITGIVAFLMCIQAKQLLLS
                     FVPGTFIGACATFAGQGDWKLVLPSLALGLVFGYAMKNSGLWLAARSAKTAHREQEIK
                     NKA"
     gene            complement(4300..4854)
                     /gene="ycdY"
                     /locus_tag="OO357_003695"
     CDS             complement(4300..4854)
                     /gene="ycdY"
                     /locus_tag="OO357_003695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone YcdY"
                     /protein_id="HCR5888497.1"
                     /translation="MNEFSILCRVLGSLYYRQPQDPLLVPLFTLIREGKLAANWPLEQ
                     DELLTRLQKSCDMTQVSADYNALFIGDECAVPPYRSAWVEDATEAEVRAFLSERGMLL
                     ADTPADHIGTLLLAASWLEDQSTEDESEALETLFSEYLLPWCGAFLGKVEAHATTPFW
                     RTMAPLTRDAISAMWDELEEDSEE"
     gene            complement(4878..5615)
                     /gene="ycdX"
                     /locus_tag="OO357_003696"
     CDS             complement(4878..5615)
                     /gene="ycdX"
                     /locus_tag="OO357_003696"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding phosphatase"
                     /protein_id="HCR5888498.1"
                     /translation="MYPVDLHMHTVASTHAYSTLSDYIAQAKQKGIKLFAITDHGPDM
                     EDAPHHWHFINMRIWPRVVDGVGILRGIEANIKNVDGEIDCSGKMFDSLDLIIAGFHE
                     PVFAPHDKATNTQAMISTIASGNVHIISHPGNPKYEIDVKAVAEAAAKHQVALEINNS
                     SFLHSRKGSEDNCRAVAAAVRDAGGWVALGSDSHTAFTMGEFEECLKILDAVDFPLER
                     ILNVSPRRLLNFLESRGMAPIAEFADL"
     gene            complement(5670..6608)
                     /gene="ghrA"
                     /locus_tag="OO357_003697"
                     /pseudo
     CDS             complement(5670..6608)
                     /gene="ghrA"
                     /locus_tag="OO357_003697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415551.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase GhrA"
     gene            6843..6930
                     /locus_tag="OO357_003698"
     tRNA            6843..6930
                     /locus_tag="OO357_003698"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6877..6879,aa:Ser,seq:gga)
     gene            complement(7080..7361)
                     /locus_tag="OO357_003699"
                     /pseudo
     CDS             complement(7080..7361)
                     /locus_tag="OO357_003699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            complement(7643..>7892)
                     /locus_tag="OO357_003700"
     CDS             complement(7643..>7892)
                     /locus_tag="OO357_003700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5888499.1"
                     /translation="YSTGGKSTLLGISKRGDKNLRRLLVQCARSFIMRLERKHGKLAE
                     WVREQLNKKHSNVVTCALANKLARIAWAITTQKKEYQA"
BASE COUNT         2143 a         1895 c         1709 g         2145 t
ORIGIN      
        1 cgccaccacc gtggttaccg ccgccgccgt actgaggaac aacacctgcc agagcgctac
       61 cggagaatac gattgctgca attgctgcta cttttaaaag tttcatgtaa aacccccatc
      121 ggattgattt aaaagtcgaa tggtaattaa cgttgtgtca cgcgaattgc catttgcgac
      181 tgtctctgca ctacaactgc cgttttttga gtaccatact gtgtaatatt tgctttatta
      241 ccagaacctt tctggataat catcgcagta ttaccataag caccttgcga aatactggca
      301 tcgttggcac tgcccgcctg atcaatatat gcaaggttat aatctcctgt ctggtcaatc
      361 tttgcccgat tgctactacc ttcttgcgca acaaccgcca aaagttttga gcctccctgc
      421 cgtaactgag cactattatt agtcccagcc tgaccaatta tggctgcctg attaaatgaa
      481 gacttactca attcatttac cgcgaagtta tattctgaat tagctaaatc ataacctgct
      541 gcggctgcaa tcccaggcgc acccagtatt gttaacatca taaataacaa tttgtttttc
      601 atgttgtcac cctggacctg gtcgtacatt taagaaatta aatcatttca acttggttgt
      661 taacgcaacc tgtattttgt taacgctgcg ttacgatgga aagtatgtct gcggaaatat
      721 ttttaataac tcacccgcgc gttgtatttt tctttttttg ttcataccaa agtattaaaa
      781 cccgcgtaaa tacgctgtta tctacgcaaa aaatattttg ttttctctta aatctccgtt
      841 ttccgctatc aaaaatcacc agacagtcat tcttcttgcc cgtcgctgat tgctgcgata
      901 tgtcttgcgc acaagccgtg acaaacctcg tctttttgca gtaaaaaaat tgtccacgga
      961 ggtgtggaga aaaaacaaaa acgttttaca tgacgaaagg actacaccga aatatttttt
     1021 atatgtatta ttagtaagtt agcaccattt gtatgatttt ttaaaattgt gcaataaaaa
     1081 ccaaatgtac aacttttcta tcatttctaa acttaataaa accttaatgt taacatttta
     1141 atataatgag ttacattttg ttacatgttt aacacttgat ttaagatttg taatggctag
     1201 attgaaatca gatgtaatcc attagtttta tattttaccc atttagggct gatttattac
     1261 tacacacagc agtgcaacat ctgtcagtac ttctggtgct tctattttag aggcagctgt
     1321 caggtgtgcg atcaataaaa aagtggggtt tcatcatgtt taatgaagtc catagtattc
     1381 atggtcatac attattgttg atcactaaac cttctttgca ggcgacagct ctcttgcagc
     1441 accttaaaca atcgctagca attacaggaa aattacataa tattcaacgt tctctggacg
     1501 atatctcttc aggctctatt attcttctgg atatgatgga agcggataaa aagcttatcc
     1561 attattggca ggatactttg agcaggaaaa acaacaatat caaaatattg ttgctaaata
     1621 cgcctgaaga ttacccgtac cgcgacattg aaaactggcc tcatatcaac ggcgtttttt
     1681 atgccatgga ggatcaagaa cgtgttgtca atgggttgca aggcgtcctg cgtggcgaat
     1741 gctactttac gcaaaagctt gccagttacc tgattacaca ttctggtaac tatcgttata
     1801 acagcacgga atcagccctc cttactcatc gggaaaaaga gatcctgaat aagctgcgta
     1861 tcggcgcgtc taataacgag atcgctcgtt cgttgttcat cagcgaaaat acggttaaaa
     1921 cgcatcttta taatcttttc aagaagatag ccgtaaaaaa ccggacacaa gcggtttcct
     1981 gggcaaacga taacctcagg cgataaagcc atgaaacgtt atttacgctg gattgtggcg
     2041 gcagaatttc tgttcgccgc agggaatctt cacgccgttg aggtagaagt cccgggattg
     2101 ttaactgacc atactgtttc atctattggc catgattttt accgagcctt tagtgataaa
     2161 tgggaaagtg actatacggg taacttaacg attaatgaaa ggcccagtgc acgatgggga
     2221 agctggatca caataacggt caatcaggat gttattttcc agactttttt attcccgttg
     2281 aaaagagact ccgagaaaac tgtcgtcttt gcactgattc aaactgaaga agcactaaat
     2341 cgtcgccaga taaatcaggc gttattaagt acggacgatt tggcgcatga tgaattctaa
     2401 ataaaaaatt gttcggaggc tgcaatgcgt gtcaaacatg cagtagttct actcatgctt
     2461 atttcgccat taagttgggc tggaaccatg actttccagt tccgtaatcc aaactttggt
     2521 ggtaacccaa ataatggcgc ttttttatta aatagcgctc aggcccaaaa ctcttataaa
     2581 gatccgagct ataacgatga ctttggtatt gaaacaccct cagcgttaga taactttact
     2641 caggccatcc agtcacaaat tttaggtggg ctactgtcga atattaatac cggtaaaccg
     2701 ggccgcatgg tgaccaacga ttatattgtt gatattgcta accgcgatgg tcaattgcag
     2761 ttgaacgtga cagatcgtaa aaccggacaa acctcgacca tccaggtttc gggtttacaa
     2821 aataactcaa ccgattttta agccccagct tcataaggaa aataatcatg cagcgcttat
     2881 ttcttttggt tgccgtcatg ttactgagcg gatgcttaac cgccccgcct aaagaagccg
     2941 ccagaccgac attaatgcct cgtgctcaga gctacaaaga tttgacccat ctgccagcgc
     3001 cgacgggtaa gatctttgtt tcggtataca acattcagga cgaaaccggg caatttaaac
     3061 cctacccggc aagtaacttc tccactgctg ttccgcaaag cgccagggct tacaaaacct
     3121 gcttaacgag cgcaaggtta ttcgtgcggc acaagaaaac ggcacggttg ccattaataa
     3181 ccgaatcccg ctgcaatctt taacggcggc aaatattatg gttgaaggtt cgattatcgg
     3241 ttatgaaagc aacgttaaat cgggcggtgt tggggcaaga tattttggga tcggtgcaga
     3301 cacgcaatac cagctcgatc agattgccgt gaacctgcgt gtcgtcaatg tgagtaccgg
     3361 tgagatcctc tcctcggtga acaccagtaa gacgatactt tcctatgaag ttcaggccgg
     3421 ggttttccgc tttattgact accagcgctt gctggaaggg gaagtgggtt acacctcaaa
     3481 cgaacccgtt atgctgtgtc tgatgtcggc tatcgaaaca ggggtcattt tcctgattaa
     3541 tgatggtatc gaccgtggtc tgtgggattt gtaaaataaa gcagaacggc agaatgacat
     3601 tctggtgaaa taccgccata tgtcggttcc accggaatcc tgacagataa aaatgccgga
     3661 attaataatc cggcatttac tgtttaagcg aaagcgtgag tgcccctcac gctttatttt
     3721 tgatttcctg ctcacggtga gccgtctttg cactacgcgc cgccagccac aggccgctgt
     3781 ttttcattgc gtaaccaaat accagcccaa gcgccagcga aggtaacacc agtttccagt
     3841 caccctgccc ggcaaacgtc gcgcatgcgc ctataaacgt ccccggaaca aatgaaagca
     3901 gcagctgttt ggcctgaata cacatcagaa acgcgacaat accggttatg acataaccga
     3961 gaatttccag atgtggtgcc agtgcactac cgtaaataat gaccatcgcc cacaccacgc
     4021 cactaagcag ggttgcagcg gagatcgcca gccctttcag cccaccttgc gggcaagcaa
     4081 agtaggccgt acagcccagg aagcccgccc agcttagtaa gcccagggaa acagccaccc
     4141 atccccagat accggagaga atgcctgttg tgattgcaat agagagaagt atgttcatgc
     4201 cgcgcatgat agcagaaaac gcggccatga atgagatcga gcacacattt tatgcaacaa
     4261 caaattatct gttgcagtta tgagtgacga tgatcacatt tactcttcag aatcttcttc
     4321 cagctcgtcc cacattgcac taatggcatc acgggttagc ggtgccatgg tgcgccagaa
     4381 aggcgtggtt gcatgggcct ccactttgcc gaggaacgca ccacaccagg gtaacagata
     4441 ctcactgaac agtgtttcca gtgcttcgct ctcatcttcc gttgactgat cttccagcca
     4501 ggaagccgca agcagcaatg tgccgatgtg atcggctggc gtatccgcca gtagcatccc
     4561 tcgttcggaa agaaaagcgc gcacttccgc ttccgttgcg tcctcaaccc atgcgctacg
     4621 atatggcggc acggcacatt catcgccgat aaacaacgca ttgtaatcgg cagagacttg
     4681 cgtcatatca cagcttttct gtaaacgcgt cagtaactca tcctgctcca gtggccagtt
     4741 cgcagccagt ttcccctcac gaatcagagt aaacagcggc accagtaaag gatcttgcgg
     4801 ttggcggtaa tagagcgaac ccagcacacg acagaggata gaaaactcgt tcattaatat
     4861 atttccatta ctagtaatta aaggtctgca aattccgcaa tcggtgccat accgcgagat
     4921 tcaaggaagt tcagtaagcg gcgcggagaa acattcaaaa tgcgctctag cggaaaatct
     4981 accgcgtcga ggattttaag acactcttca aattccccca tggtaaacgc ggtgtgagaa
     5041 tccgagccta atgccaccca acctccagca tcacgtaccg ctgcagctac cgcacgacag
     5101 ttgtcttcac tgcccttacg tgagtgtaaa aatgaggaat tattgatttc cagcgccacc
     5161 tgatgtttcg cggctgcttc agcaacggct ttaacatcta tttcatattt cggatttccg
     5221 ggatggctta ttatatgcac attgccgctg gcgatagtgg agatcatcgc ttgtgtattg
     5281 gtcgctttgt catgtggcgc aaaaaccggc tcatgaaaac cggcaataat cagatccagc
     5341 gagtcaaaca ttttaccgct gcagtcaatt tcaccatcaa catttttaat gttagcttca
     5401 atgccgcgca ggatccctac cccatcaacc actcgcggcc agatacgcat gttaatgaag
     5461 tgccagtgat gcggcgcatc ttccatatcc gggccatgat cggtgatcgc aaaaagttta
     5521 atgccctttt gtttggcctg ggcaatgtaa tcactcaatg tgctatatgc atgtgtgctg
     5581 gcaacggtat gcatatgaag gtcgacggga tacataactc tctcctgtat tcaattcccg
     5641 ccaaggatag caggaatcct gatgctttat tagtagccac gtgcgcggtc gacttgcccg
     5701 cagaccctct cccctttttc gagctgggca atagtgcgag aaatgtactc cacagcttca
     5761 gcgggacggg taatcgcggc gacatgtggt gttatcgtca cgcgtggatg ttgccagagc
     5821 ggactttcag gcggtaaggg ttcacgatta aaaacatcca gcattgcgcc tttaactttc
     5881 ccgctatcca gcgccgcgag caggtcatct tccacaacat gaacaccacg cgccaggttg
     5941 aggagatacg cgccatcagg taatttttcg agtaattgtt gattaataat gccgacggtt
     6001 tcaggggtat tcggtaacaa attaatcaat acccgacatt ggctcagaaa tgcagacagt
     6061 tcttcccgtc cggcaaagct ttgcacgcca ggccacgatt tacgggttcg actccagcaa
     6121 cgcagcggaa agcgctaggt ttgcagactc tgagcaactt tacagcccag tacgcctgcg
     6181 cccaaaatgc cgatggtaaa atcttcccga tgatattcag gcagcggttg ccaatgcgaa
     6241 ctattttgct ggatgcgata atcgtcaaaa cgtcgaaacc aatgcagcac ctgactgaca
     6301 gcatattcct gcatttgctc gcccataccg gtatcttcca ggcgaaaaag tggaacagaa
     6361 gggttcagca tttcagggtg tgcctgtagc ttgctcaaaa tagaatcaac accggccccg
     6421 agtgcgaaca ccgctttaag atcgcgccct gccagcattt caacaggagg atgccagact
     6481 aaaacataat cagcagagtc attatctccg cttttccatg ccctgactct tgcctgagga
     6541 atagctttgc gcagtgcctc aatccaccat tgggtatcga acgttgggtg ataaaagatg
     6601 atatccatac tgactcccga aaagcgttgt gcgaaattta ttcgcactta tcgttatgat
     6661 ctacaaaggc caccagcata acaaatccgt ggtcggtggc aaaaaaagca gatttcgctt
     6721 attaaaacca cacattgatt gaatgtttga ataaacgcgc gattttttca aaaagtttgt
     6781 tgacctcagg tcatgatttc cctaaattag cgcccgttcc agcaagacag gaacgacaat
     6841 ttggtgaggt gtccgagtgg ctgaaggagc acgcctggaa agtgtgtata cggcaacgta
     6901 tcgggggttc gaatcccccc ctcaccgcca gattataagt gcatgttatt gtttaacatg
     6961 cgctttttta tttttccttc cagacagtat gccatcgggt atgccatctg aaaccggttt
     7021 atatgccccg cgatcatatc ttatcggaca atttccgatc tttgagcata ctggcaaagc
     7081 tatactgcca gcatatcagg atagtgtttt gcgataatat cattcagtct gtcaaccagt
     7141 tctggttttc tgaacgtgat atgcgcggag cccttcttaa agtatctgat gctgaacatc
     7201 tcatcttcat agctctcttt accctgaaca gactgaatgt gatcatccag acgaagggtg
     7261 atgtttcccc ggttgtcggg gattggttta ccgctgaaca ggtgcaacat tctttccaga
     7321 tcggcgagtc ggtcagcctg ttgtccggtg ataagatgaa atctatggtc acccccgttt
     7381 ttgcaacact gatttttgat taatggttgg cttgcttaaa tctatccggc gtctctgtgg
     7441 gcaaggatgc ccgcgccccg atgagttccg cacctgataa tcctaaaaat cccctcggct
     7501 tcagtgagcc atttcatctg ttaggttttt aatcaggtcg atttgccgtt catgtcatca
     7561 ataatcagtc ttcgcaaaac cagaaaggta acttgtatct tattgtttaa aataaactaa
     7621 ttgagttata tcagcatcta tatcaggcct gatattcttt tttctgagta gtgattgccc
     7681 aggcaattcg agccagttta tttgccagag cgcaggtaac cacattcgaa tgcttcttgt
     7741 tgagttgctc ccgaacccac tcagcaagtt ttccatgctt gcgttcaagt cgcattatga
     7801 atgagcgggc acactgaacc agtaaccggc gtaaattttt atcacctcgc ttactgatac
     7861 ccaacagagt cgattttcct ccagtgctat ac
//
LOCUS       DAJTRC010000149         7859 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.149, whole genome shotgun sequence.
ACCESSION   DAJTRC010000149 DAJTRC010000000
VERSION     DAJTRC010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7859)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7859)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7859
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.149"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(1..86)
                     /locus_tag="OO357_003701"
                     /pseudo
     CDS             complement(1..86)
                     /locus_tag="OO357_003701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            84..408
                     /locus_tag="OO357_003702"
                     /pseudo
     CDS             84..408
                     /locus_tag="OO357_003702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612087.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(438..3350)
                     /locus_tag="OO357_003703"
     CDS             complement(438..3350)
                     /locus_tag="OO357_003703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888500.1"
                     /translation="MFSKAFLRKISMFFARRKPAAMKICLYHTLNPDTIPGYKKFAQA
                     IATDNFVQADVRKIDTNLYRARLSIRDRLLFSLYRYHGETICLVLEYIRNHAYNTSRF
                     LRRNVVIDEGRLQQQPVPDPVDIATEALTYINPSHGRFHRLDKMLSFDDDQQALYEHP
                     LPLVIVGSAGSGKTALVLEKMKQAAGDILYLSLSSFLVEKARTLYDASGEGSEVQNID
                     FLSLTEFLETLRIPEGREVTFSAFSDWLPRNRAIAALGAAHTLYEEFRGVIGAVASGN
                     GPLSREAYLSLGIRQSLYGMEDRPTVYVLFERYIAWLKQSHQYDSNLLSHQYLSLATP
                     RYDVIFVDEVQDMTPVQLQLVLKTLRHPGQFLLCGDANQIVHPSFFSWSSLKSLFFRQ
                     QQGNDTTVNILQANYRNGHHVTALANRLLRLKQVRFSAIDRESHHFVRSCGQAEGTIR
                     LLDDREETKQELNAKTSLSNRVAVIVMHPEQKAQARCWFSTPLVFSVQEVKGLEYETV
                     ILYNIVSAARQAFDDICEGLTPADLEGEARYSRPRDRQDRSAEIYKFFTNALYVALTR
                     AKHNVYLVEQQVEHPLWSLLALTHQEEPLNLQEEISSRDEWQKTAHLLEKQGKQEQAD
                     TIRSRILQTSEMPWQIITAEDARQWKQHILAGTADKTIQLQALEYSLIYSLFPLYNAL
                     YREDFKPTRQPRTKTLQLLELKYFRPYSMNNPVAVLRDIERYGVDHRSPFNLTPLMSA
                     ARAGNIALVQLLLERGADPLLTGNDGLAAYHQVLSAAVSTPRYAQQKSAQLYTLLKPE
                     SLSLQVEGRLIKLDNRQMAMFLVILMQALFHTHLGSALFFSEAFSAARLAECVVHLPE
                     ALLPERRKRRSYISSQLSQHEVNSKNPYGKKLFLRLNHGQYILNPGLKIRKGDVWRAV
                     YELQSPEDLGHDLQTYLQDMSPELVDMLGGKKGFYERSEKSVGYWVGGIRRAAQKA"
     gene            3353..3469
                     /locus_tag="OO357_003704"
     CDS             3353..3469
                     /locus_tag="OO357_003704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004996477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HCR5888501.1"
                     /translation="MPGIPPEIRWNNQKNALQLWNKEGGVVRREGDPLEMAQ"
     gene            3833..4675
                     /locus_tag="OO357_003705"
     CDS             3833..4675
                     /locus_tag="OO357_003705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HCR5888502.1"
                     /translation="MLNEGGILFKANHVPVLMYHHVSHCPGLVTLSPVTFRKQIKWLA
                     ENNWKTLSSDELEFFYRGGKLPRKSVMLTFDDGYLDNWFQVYPLLKEFNLKAHIFLIT
                     GFIGNGPVRHSPGKEYSHRDCEHQIATGNADNVMLRWSEVNEMLQSGLVEFHVHTHTH
                     TRWDKKFSSREEQCKHLRQDLLSGREYLKEMTGKCSKHLCWPEGYYNKDYIQVAEELG
                     FYYLYTTERRMNAPAKGTTRIGRISTKERESCAWLKRRLFYYTTPFFSSLLAFHKGPR
                     LPDD"
     gene            4678..5766
                     /gene="capU"
                     /locus_tag="OO357_003706"
     CDS             4678..5766
                     /gene="capU"
                     /locus_tag="OO357_003706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative hexosyltransferase CapU"
                     /protein_id="HCR5888503.1"
                     /translation="MNILFTESSPNIGGQELQAVAQMKALKKMGHSVLLVCRENSKIA
                     FEASKLGIDITFALFRNSLHIPTAWRLLGIVHGFQPNAIVCHSGHDSNIVGLVRLFTR
                     KHPFRIIRQKTYLTRKTKVFSINHFCDEVIVPGTSMKTHLEQEGCRTRVTVVPPGFDF
                     QKLYVDSRNSLPPNVLSWLASRRGCPVIAQVGMLRPEKGHEFMLNLLFHLKMNGRQFC
                     WLIVGSGSPELREHLQYQIDSMGMHDDVFIADNVFPAAPVYRVASLVVLPSENESFGM
                     VLAEASAFSVPVLASQIGGIPDVIQNNQTGTLLPAGNKHAWMCALNDFFNDPGRFYQM
                     ARQAKQDIEERFDINKTALKILTLAKHK"
     gene            5771..6721
                     /gene="virK"
                     /locus_tag="OO357_003707"
     CDS             5771..6721
                     /gene="virK"
                     /locus_tag="OO357_003707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5888504.1"
                     /translation="MFSVSNLSFIGFLKRIVFSSDSLPGKWEHRKFRFMYILRCAINP
                     VASIRYYYELRSLQCIEDILAIQPTLPARIHRPYLHKGGRAWSRGQYILEHYRFVQNL
                     PEKYSEFLFPQKSVSLVQFIGKDGEDFDIQCSPSGFDREGELMLSLFFNKIVIARLTF
                     SVILTQNGHTAFIGGLQGAPKNTGPDIIRCATRACYGLFPKRIIFEAFCALMKACNVS
                     ECLAVSEHSHVFRQLRYWYQKRKTFVAVYSDFWESVAGKTCGDWYKLPTQVVRKPLSN
                     IASKKRSEYRKRYALLDYIHETAIRSLDAYPVNSEHQDLN"
     gene            6786..7730
                     /gene="lpxM"
                     /locus_tag="OO357_003708"
                     /gene_synonym="msbB"
     CDS             6786..7730
                     /gene="lpxM"
                     /locus_tag="OO357_003708"
                     /gene_synonym="msbB"
                     /EC_number="2.3.1.243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="LpxM is lauroyl-Kdo(2)-lipid IV(A)
                     myristoyltransferase, an enzyme characterized in
                     Escherichia coli and involved in biosynthesis of the form
                     of lipid A found in that species and some closely related
                     species; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5888505.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWFTWFVLGMIAGISMFPPSFRDP
                     VLAKIGRWVGRLSRKARRRATINLSLCFPEKSDTEREIIVDNMFATALQSIVMMAELA
                     IRGPEKFQKRVFWKGLEILEEIRHNNRNVIFLVPHGWSVDIPAMLLAAQGEKMAAMFH
                     QQRNPVIDYVWNSVRRKFGGRLHSREDGIKPFIQSVRQGYWGYYLPDQDHGPEYSEFA
                     DFFATYKATLPIIGRLMNISQAMIIPLFPVYDEKKHFLTIEVRPPMDACIASADNKMI
                     ARQMNKTVEILVGSHPEQYIWVLKLLKTRKSNEADPYP"
BASE COUNT         2116 a         1759 c         1860 g         2124 t
ORIGIN      
        1 tcctgctccg acatacctgc aggcaaaggc catgaaagcc ctgatttccg gaagcgagcg
       61 aacagtccac aaactgttga acgtggtgtc agcggcggac agggataagc cctcgtcagc
      121 agttccgtca gcactgcgac agcgttcttt acccggtcaa aacagcgttg cggtgcacca
      181 cgcctgacgg atgaactgcg tgctcagggt taccacttta acgtaaaaac cgtggcggca
      241 agcctgcgcc gtcaggggct gagggcaaag gcctcccgga agttcagccc ggtcagttac
      301 cgcgcacatg gcctgcccgt gtcagaaaat ctgctggagc aggattttta cgccagtggt
      361 ccgaaccaga agtgggcggg agacatcacg tacttacgta cagatgaagt tcggttacat
      421 cctgtcagca cggaacctca cgctttttga gcggcacgac ggatcccccc cacccagtat
      481 ccgacagact tttcactgcg ttcgtaaaaa cccttcttcc ctcccagcat atccactaat
      541 tccggtgaca tatcctgcag ataagtctgc aaatcatgcc ccagatcttc cggtgactgc
      601 aactcataaa ccgcgcgcca cacatcaccc ttccttattt taagccccgg gttaagaata
      661 tactgtccgt ggttcagcct cagaaaaagc ttttttccat aaggattttt gctgttaact
      721 tcatgttgag agagctggct gctgatataa ctccttcgtt tgcggcgttc cggcagcaac
      781 gcttcaggaa gatgaaccac acactctgcc agtcgtgccg cactgaaggc ttccgaaaaa
      841 aacagagcac ttcccagatg cgtgtggaac agcgcctgca tcaggatcac cagaaacatt
      901 gccatctgcc ggttgtccag tttgatgagg cgtccttcca cctgcagtga cagactttca
      961 ggtttcagca gggtatacaa ctgcgcactt ttctgttgcg cataacgggg cgtactgacc
     1021 gccgctgaca gaacttgatg ataagccgcc agcccgtcat taccggtcag caagggatct
     1081 gcaccacgct ccagcagtaa ctgaaccagt gcaatattac ctgcccgggc tgctgacatc
     1141 agcggagtaa gattaaacgg cgaacggtga tcaacaccat aacgttcaat atcccgcaac
     1201 acggcaaccg ggttattcat tgagtatggc cggaaatact tcagctccag taactgcaga
     1261 gtcttcgttc tgggctgcct tgtgggctta aaatcttccc ggtacagtgc attatacagc
     1321 gggaacaggg aataaatcag actgtactcc agcgcctgca gttgtatggt tttatcggct
     1381 gtacctgcga ggatatgctg tttccactgt cgtgcatcct ctgcggtgat aatctgccat
     1441 ggcatctcag atgtctgcag gatccggcta cggatggtgt ccgcctgctc ctgcttcccc
     1501 tgtttttcaa gcagatgtgc ggttttctgc cactcatcac ggctggaaat ctcttcctgc
     1561 agattcagag gctcttcctg atgcgtgagc gcaagcagac tccacagagg atgctccacc
     1621 tgctgttcaa caagatacac attgtgttta gcacgggtga gcgcaacata cagcgcgttg
     1681 gtaaaaaatt tatagatttc cgctgaacga tcctgcctgt cccgcggacg ggaataacgg
     1741 gcctcgccct caagatcagc cggtgtaaga ccctcgcaaa tatcatcaaa tgcctgccgg
     1801 gcagcgctta ctatattgta aaggatcacc gtttcgtatt ccagcccctt aacctcctga
     1861 accgaaaaga ccagcggcgt actgaaccag catcgcgcct gtgctttctg ttccggatgc
     1921 atcacaatga ccgctacacg attcgaaagt gaggttttgg cattcagttc ctgctttgtc
     1981 tcttccctat cgtccagcaa ccggatggtc ccttctgcct gaccgcagct tctgacaaaa
     2041 tggtgacttt cccgatcaat agcactaaag cggacctgtt tgagtctcag caaccggtta
     2101 gccagtgcag tgacgtggtg cccgttacgg taattcgcct gcaggatatt aacggttgtg
     2161 tcgtttccct gttgctgccg gaaaaagaga cttttgagcg aactccatga gaaaaagctc
     2221 ggatgaacta tctgattggc atcaccacag agtaaaaact gaccgggatg acgtagcgtt
     2281 ttcagtacca gttgcagttg aacaggcgtc atatcctgaa cttcatcaac aaaaatgaca
     2341 tcataacggg gagttgccaa cgaaagatac tgatgactga gcagattact gtcatactga
     2401 tgcgactgtt tcagccatgc gatataacgt tcaaacagga catagaccgt cgggcggtcc
     2461 tccatgccat agagcgactg ccgtatcccc agtgacagat acgcctcccg ggacagcggc
     2521 ccattcccgg aggcaaccgc accgatcacg cccctgaact cctcatacag ggtatgggca
     2581 gcccccagtg cggcgatagc cctgttgcgg ggcagccagt cgctgaacgc actgaaagtc
     2641 acctccctgc cttccggaat gcgcagagtt tccagaaatt ccgtcagcga aagaaagtca
     2701 atattctgta cctcactccc ttcccctgaa gcgtcataga gagtacgcgc tttttccacc
     2761 agaaatgagg aaaggctgag atacagtata tcaccagcag cctgcttcat tttttccagt
     2821 accagcgccg tcttaccact tcccgccgag ccaacaatca ccagcggtag cgggtgttcg
     2881 tacagtgcct gctgatcatc atcaaaggac agcattttat ccaggcggtg gaaacgccca
     2941 tgagacggat tgatatacgt cagcgcttca gtcgcgatat caacaggatc cggcactggt
     3001 tgttgttgta agcgcccttc atcaatcacc acgtttctgc gaagaaaacg tgaagtgtta
     3061 taggcatgat tgcgaatata ttccagaaca aggcagatag tttcaccgtg gtagcgatac
     3121 agcgaaaaca gtaatcggtc ccggatactg agccgggcgc ggtaaagatt ggtgtcaatt
     3181 ttacgaacat cagcctgaac aaagttatcg gtggcaatag cctgtgcaaa ttttttataa
     3241 cccgggatcg tgtcaggatt cagagtatga taaagacaga ttttcatggc tgccggtttc
     3301 cgtcgtgcaa aaaacatgct gattttacga aggaatgcct tactgaacaa ccttgcccgg
     3361 aatacccccc gaaatcagat ggaacaatca gaaaaatgct ctgcagttat ggaataaaga
     3421 aggtggggtg gtacgccggg agggcgatcc gctggaaatg gcgcagtagc tgcaggcctg
     3481 tcatgatgca ggaatagaag ttcgtgtaca ggttaatgaa agcgtcaccc cgtaaataaa
     3541 ctctgcgctg aaattccttt ttttcattgg agtacctgtg ttgttctgag gtgaacatat
     3601 cacctctgtt caggtagcca aattcagtgt gccacctcaa ggttcttagt gagtcgtttt
     3661 taaaagatat gataatatat cattgtttat gaattgttga tgtttaattt agtaaattat
     3721 atcaaatatg ccatgggttt gctatgttat tcatccagta tctggaaata aaatgcacca
     3781 gctttattgg gttttccttt tgtctctgct tgctaggtgc cagaaacttt aaatgttaaa
     3841 cgaagggggc attttgttca aagccaatca tgttccggta ttaatgtatc atcatgtcag
     3901 tcattgcccc ggactggtta cactttctcc cgtgacattc cggaagcaaa taaaatggtt
     3961 ggctgaaaac aactggaaaa cactgtcatc agatgaactg gagttttttt atcggggcgg
     4021 aaaactcccc cggaaaagcg ttatgttgac atttgatgat ggataccttg acaactggtt
     4081 tcaggtatat cctttattaa aagagtttaa tctcaaagca catatttttt taattaccgg
     4141 ttttattggg aatggtccgg ttcgtcactc tcctggaaag gaatattctc atcgcgattg
     4201 cgaacatcaa atagcgacag ggaatgctga taatgttatg cttcgctggt ctgaagtaaa
     4261 cgaaatgttg caatctggtc tggtcgaatt tcatgttcat acgcatactc atacacgctg
     4321 ggataaaaag ttcagttcac gagaagaaca gtgtaaacat cttcggcagg acttgttgag
     4381 cgggagggaa tatttgaaag aaatgacagg aaaatgcagt aaacatttgt gttggccgga
     4441 aggttattat aacaaggatt atattcaggt cgctgaagaa cttggattct attatttata
     4501 tacaacagaa agaagaatga atgctcctgc taaaggaact acacggatag gaagaataag
     4561 caccaaagaa cgggaaagtt gcgcatggct gaaaagacga cttttttatt atacaacccc
     4621 gtttttttca tcgttgctgg cctttcataa ggggccaaga ttaccggatg attaataatg
     4681 aatatactat ttacggaatc atcaccgaat ataggtggtc aggaattaca ggctgttgct
     4741 caaatgaagg ccctgaagaa aatggggcat tcagttctgc ttgtctgcag ggaaaacagc
     4801 aaaattgctt ttgaagccag taaattgggg attgatatca cattcgcgtt atttcgaaac
     4861 agtcttcaca tccctactgc atggagatta ctcggaatag ttcatggttt tcagcccaat
     4921 gcaatcgttt gtcacagtgg gcatgatagc aatattgttg gtttagtacg gttattcact
     4981 aggaaacatc cattcagaat tatcaggcaa aagacatatt tgacacgaaa aacaaaagtt
     5041 ttttcaataa atcatttttg cgatgaggtg attgttcccg gaacaagtat gaagacacat
     5101 ctggagcagg aaggatgtcg aacccgggtt actgttgtgc ctccaggctt tgatttccag
     5161 aaattatacg ttgattcccg aaacagtttg cctccaaatg ttctttcttg gctggcgtcc
     5221 cgaaggggat gccccgttat tgctcaggta ggaatgttgc gcccggaaaa agggcacgaa
     5281 tttatgttga atttactgtt ccatttaaaa atgaatggac gacagttctg ttggttgatt
     5341 gtggggtctg gttcgcctga actgcgggag catttacagt atcagattga cagtatgggc
     5401 atgcatgatg atgtttttat tgctgacaat gtttttcctg ccgcccccgt atatcgggtt
     5461 gccagtctgg tggttctgcc ttcagaaaac gaatcgtttg gtatggtact ggcagaagca
     5521 tcggcatttt ctgtgcctgt actggccagt cagattggtg gaatccctga tgttattcag
     5581 aataaccaga ccgggacatt gttaccagca ggtaataagc acgcatggat gtgcgccctg
     5641 aatgattttt ttaatgaccc tgggcgtttt tatcagatgg ctcgccaggc aaaacaggat
     5701 atagaagagc ggtttgatat taataaaact gcgttaaaaa tactcacatt agcgaagcac
     5761 aagtaacaat atgttttctg taagtaactt atcatttatc ggtttcctta aaaggattgt
     5821 tttttcctca gattcactcc cggggaagtg ggaacacaga aaattccggt tcatgtacat
     5881 tttgcgatgt gctataaatc cggttgccag tattcgatat tattacgaac tgcgttcctt
     5941 gcagtgcatt gaggatattc tggcaataca acccacgttg ccagcaagaa ttcatcgacc
     6001 ttatttgcat aaaggggggc gagcctggtc ccgggggcaa tatattcttg agcattatcg
     6061 ctttgtgcaa aatttgccag aaaaatattc tgaatttttg tttccacaaa aatcggtatc
     6121 attagtccaa ttcatcggga aagatggaga ggattttgat atccagtgtt ctcccagtgg
     6181 ttttgaccgg gaaggagagt tgatgttgtc attgtttttc aataaaatag tgattgcccg
     6241 gttaacattt tcagtcattc tgactcaaaa tggacacacc gcttttattg gcggattaca
     6301 gggagccccc aaaaataccg gacctgatat tattcggtgc gcaacccggg cctgttatgg
     6361 gcttttccca aaacggatta tttttgaagc attttgtgcg ttgatgaaag cttgtaatgt
     6421 ctctgagtgt ctggcagtca gtgagcacag tcatgttttc cggcaattga gatactggta
     6481 tcagaaacgc aaaacctttg ttgctgtcta cagtgatttc tgggagtctg tagcagggaa
     6541 aacctgtggt gactggtaca aattaccaac acaggtggtt cgaaagccgc taagcaatat
     6601 agctagtaaa aaacgctctg agtatcgaaa acgatacgct ttgctggatt atatccatga
     6661 aactgctatc cgctctttgg atgcatatcc tgtaaactca gaacatcagg acttaaatta
     6721 aggtttgatg tattctctga ataaaatatt aatgatgatt ttggtagggg cattcgcact
     6781 aaataatgaa aaaatacaaa tccgagttta ttcctgaatt taagaaaaat tatctttccc
     6841 ctgtttactg gtttacatgg ttcgttttgg gaatgattgc aggtatttca atgtttcccc
     6901 cttcattcag agatcctgtc ttggccaaaa tagggcgttg ggtgggcaga ttgagcagaa
     6961 aagctcgtcg cagggcgacg attaatttat cgctttgttt cccggaaaag agtgatacag
     7021 aacgggaaat aattgtcgac aatatgtttg ccacagcatt gcaatccata gtgatgatgg
     7081 cagaactggc aattcgtggt ccggaaaagt tccagaaacg agtgttctgg aaaggcctcg
     7141 aaattcttga ggaaattcgg cacaataaca gaaatgtgat ttttctggtt ccccatggct
     7201 ggagcgtgga tattcctgca atgttgctgg cagctcaggg ggaaaaaatg gccgccatgt
     7261 ttcatcagca acgaaatcca gtgattgatt atgtctggaa ttctgtacgg cgtaaattcg
     7321 gggggcgctt acattcccgg gaggatggga taaaaccatt tattcagtca gtacgccagg
     7381 gatactgggg gtattacctt ccagatcagg atcatggtcc tgaatacagt gaatttgctg
     7441 atttttttgc gacctataaa gcgacattac caattattgg acgtctgatg aacatcagtc
     7501 aggctatgat tataccgctt ttcccggttt atgatgaaaa aaaacatttc ctgactattg
     7561 aagttcggcc accaatggat gcatgcattg ccagcgcgga caataaaatg attgcccgac
     7621 aaatgaacaa aacagtggaa attttggtgg ggtcacatcc ggaacagtat atctgggttt
     7681 taaaattgtt aaaaacgcgc aaatcaaacg aagcggaccc gtacccttga aattataaag
     7741 tacaggtatt tccactagtt gtttcttaca ggttaccaat cgaaacacat cccccttccg
     7801 tgtgttgccc gtttggcacg gaggactgtg tagatgcagt tcgttaaatt taatttatg
//
LOCUS       DAJTRC010000150         7829 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.150, whole genome shotgun sequence.
ACCESSION   DAJTRC010000150 DAJTRC010000000
VERSION     DAJTRC010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7829
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.150"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            1..957
                     /gene="espL1"
                     /locus_tag="OO357_003709"
                     /pseudo
     CDS             1..957
                     /gene="espL1"
                     /locus_tag="OO357_003709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310454.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type III secretion system effector EspL1"
     gene            1129..1236
                     /locus_tag="OO357_003710"
     CDS             1129..1236
                     /locus_tag="OO357_003710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_588455.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888506.1"
                     /translation="MPTKRFDKKHWKMVVVLLAICGAMLLLRWAAMIWG"
     gene            complement(1289..2047)
                     /locus_tag="OO357_003711"
     CDS             complement(1289..2047)
                     /locus_tag="OO357_003711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdiY family protein"
                     /protein_id="HCR5888507.1"
                     /translation="MKLLKTVPAIVMLAGGMFASLNAAADDSVFTVMDDPASAKKPFE
                     GNLNAGYLAQSGNTKSSSLTADTTMTWYGQTTAWSLWGNASNTSSNDERSSEKYAAGG
                     RSRFNLTDYDYLFGQASWLTDRYNGYRERDVLTAGYGRQFLNGPVHSFRFEFGPGVRY
                     DKYTDNASETQPLGYASGAYAWQLTDNAKFTQGVSVFGAEDTTLNSESALNVAINEHF
                     GLKVAYNVTWNSEPPESAPEHTDRRTTLSLGYSM"
     gene            2334..3263
                     /gene="pfkB"
                     /locus_tag="OO357_003712"
     CDS             2334..3263
                     /gene="pfkB"
                     /locus_tag="OO357_003712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707391.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase II"
                     /protein_id="HCR5888508.1"
                     /translation="MVRIYTLTLAPSLDSATITPQIYPEGKLRCTAPVFEPGGGGINV
                     ARAIAHLGGSATAIFPAGGATGEHLVSLLADENVPVATVEAKDWTRQNLHVHVEASGE
                     QYRFVMPGAALNEDEFRQLEEQVLEIESGAILVISGSLPPGVKLEKLTQLISAAQNQG
                     IRCIIDSSGEALSAALAIGNIELVKPNQKELSALVNRELTQPDDVRKAAQEIVNSGKA
                     KRVVVSLGPQGALGVDSENCIQVVPPPVKSQSTVGAGDSMVGAMTLKLAENASLEEMV
                     RFGVAAGSAATLNQGTRLCSHDDTQKIYAYLSR"
     gene            3364..3654
                     /gene="ghoS"
                     /locus_tag="OO357_003713"
     CDS             3364..3654
                     /gene="ghoS"
                     /locus_tag="OO357_003713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type V toxin-antitoxin system endoribonuclease
                     antitoxin GhoS"
                     /protein_id="HCR5888509.1"
                     /translation="MASGDLVHYVITVMLHEDTLTEINELNNYLTRDGFLLTMTDDEG
                     NIHELGTNTFGLISTQSEEEIRELVSGLTQSATGKDPEITITTWEEWNSNRK"
     gene            3760..4620
                     /locus_tag="OO357_003714"
     CDS             3760..4620
                     /locus_tag="OO357_003714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HCR5888510.1"
                     /translation="MWQAISRLLSEQLGEGEIELRNELPGGEVHAAWHLRYAGHDFFV
                     KCDERELLPGFTAEADQLELLSRSKTVTVPKVWAVGADRDYSFLVMDYLPPRPLDAHS
                     AFILGQQIARLHQWSDQPQFGLDFDNSLSTTPQPNTWQRRWSTFFAEQRIGWQLELAA
                     EKGIAFGNIDAIVEHIQQRLASHQPQPSLLHGDLWSGNCALGPDGPYIFDPACDWGDR
                     ECDLAMLPLHTEQPPQIYDGYQSVSPLPADFLERQPVYQLYTLLNRARLFGGQHLVIA
                     QQSLDRLLAA"
     gene            complement(4661..5197)
                     /locus_tag="OO357_003715"
     CDS             complement(4661..5197)
                     /locus_tag="OO357_003715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YniB family protein"
                     /protein_id="HCR5888511.1"
                     /translation="MTYQQAGRIAVLKRILGWVIFIPALISTLISLLKFMNTRQENQE
                     GINAVMLDFAHVMIDMMQANTPFLNLFWYNSPTPNFNGGVNVMFWVIFILIFVGLALQ
                     DSGARMSRQARFLREGVEDQLILEKAKGEEGLTREQIESRIVVPHHTIFLQFFSLYIL
                     PVICIAAGYVFFSLLGFI"
     gene            5344..6012
                     /gene="hxpB"
                     /locus_tag="OO357_003716"
     CDS             5344..6012
                     /gene="hxpB"
                     /locus_tag="OO357_003716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexitol phosphatase HxpB"
                     /protein_id="HCR5888512.1"
                     /translation="MSTPRQILAAIFDMDGLLIDSEPLWDRAELDVMASLGVDISRRN
                     ELPDTLGLRIDMVVDLWYARQPWNGPSRQEVVERVIARAISLVEETRPLLPGVREAVA
                     LCKEQGLLVGLASASPLHMLEKVLTMFDLRDSFDALASAEKLPYSKPHPQVYLDCAAK
                     LGVDPLTCVALEDSVNGMIASKAARMRSIVVPAPEAQNDPRFVLANVKLSSLTELTAK
                     DLLG"
     gene            6175..6765
                     /locus_tag="OO357_003717"
     CDS             6175..6765
                     /locus_tag="OO357_003717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707386.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HCR5888513.1"
                     /translation="MTAEGHLLFSIACAVFAKNAELTPVLAQGDWWHIVPSAILTCLL
                     PDIDHPKSFLGQRLKWISKPIARAFGHRGFTHSLLAVFALLATFYLKVPEGWFIPADA
                     LQGMVLGYLSHILADMLTPAGVPLLWPCRWRFRLPILVPQKGNQLERFICMALFVWSV
                     WMPHSLPENSAVRWSSQMINTLQIQFHRLIKHQVEY"
     gene            6898..>7829
                     /gene="tcyP"
                     /locus_tag="OO357_003718"
     CDS             6898..>7829
                     /gene="tcyP"
                     /locus_tag="OO357_003718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
                     /protein_id="HCR5888514.1"
                     /translation="MNFPLIANIVVFVVLLFALAQTRHKQWSLAKKVLVGLVMGVVFG
                     LALHTIYGSDSQVLKDSVQWFNIVGNGYVQLLQMIVMPLVFASILSAVARLHNASQLG
                     KISFLTIGTLLFTTLIAALVGVLVTNLFGLTAEGLVQGGAETARLNAIESNYVGKVSD
                     QSVPQLVLSFIPKNPFADLTGANPTSIISVVIFAAFLGVAALKLLKDDAPKGERVLTA
                     IDTLQSWVMKLVRLVMQLTPYGVLALMTKVVAGSNLQDIIKLGSFVVASYLGLLIMFA
                     VHGILLGINGVSPLKYFRKVWPVLTFAFTSRSSAA"
BASE COUNT         2072 a         1858 c         1850 g         2049 t
ORIGIN      
        1 atgctcagac ctgggctgat aggtatgttc gcaactgtgc aattgctatt ctgcccctat
       61 tacctgtggg agttctaggg gctattttcg cgggtattgc atcacgaatg ccatttgccc
      121 ctatacatcc atcggcaatg ttgttaataa tggctacagg ccagactcaa cagcttatta
      181 cattattcaa acagttgccc atactccctg aaaaagaaat cattgaaata ataactgcgc
      241 agaatagcgt tggtacacct gctttatttt tggctatgat gaacggacat actgacaacg
      301 tgaaaatatt tatgcaagaa attcagtcac tggtagataa tcacatcatt catgaagata
      361 atctggttaa attactgcaa actaaaagtg ctaacgaaac acctggactt tatatctcca
      421 tgttgtatgg attcgatgaa ataatcgata tctttctgaa tacattagcc actccaatag
      481 cactaagagc ttttaaacaa aaaactggtg atgagtattt tagccatgaa aatacatgat
      541 ggtgagccag gattatacgc cgcaatggaa aataatcacc ctttgtgtgt cacacggttc
      601 ctctctaaaa ttaatggcat cgcctttaaa tacaagttga gcaaagctaa catcatggat
      661 ttattaaaag gagctacagc acagggaacc cctgcattat acatcgccat gagcaagggt
      721 aatgaagacg tcgtgttatc ttatatatcg acgctgggtg cttttgcaaa aaaacattct
      781 tttagtcaac atcagttatt tacactattg gccgctaaaa atcatgacaa catgtcagct
      841 gttcatatag ccattcatca taagcattat aaaactgtag aaacatatta tgctgctata
      901 aatgtcatca gccaaagcct gagttttagt gctgatgaaa taaagacgta tttataacca
      961 gctatttacg atatatatcg atacataatt ttattttttt cataaaacgc agaaacagtg
     1021 accatgtgca caacgccacc cgacggctgt aataacaaaa aatcccttga tgcctgtccc
     1081 ttttgttaca ctccgttatc acgcacaaga gatatgcagg acactggtat gccgactaaa
     1141 cgctttgata aaaaacactg gaagatggtg gtggtgctac tggcaatctg tggcgctatg
     1201 ttgttgctac gttgggcagc aatgatttgg ggctgagcag tgacaaacga agacagccag
     1261 accgcataac acggtccggc agataatttt acatgctgta gcccagcgat aaggttgtcc
     1321 gacggtcggt atgttccggt gcagactctg gaggttcaga gttccatgtg acgttgtaag
     1381 ccaccttcag tccaaaatgt tcattaatag caacatttaa tgcgctttcg gagttcagcg
     1441 ttgtgtcttc cgcgccaaag accgaaacac cctgcgtaaa tttagcgttg tcagtcaact
     1501 gccatgcata agccccggag gcatagccca gcggctgggt ttcactggca ttgtcggtat
     1561 atttgtcgta acgcacacct ggaccgaatt caaagcggaa actgtgtacc gggccattca
     1621 aaaactgacg accataaccc gcggtcaaca catcgcgctc tcgatagccg ttataacggt
     1681 ctgtcagcca gcttgcctga ccaaataaat agtcataatc agttaaatta aagcggctac
     1741 gcccgcccgc cgcatatttt tctgaagaac gctcatcatt agaagaagta ttactggcgt
     1801 tcccccacag cgaccaggca gtggtttgtc cataccaggt catggtggtg tcagccgtaa
     1861 gtgaggagct tttcgtattg cctgattgtg caagatatcc tgcgttcaga ttaccttcga
     1921 aaggtttttt agcgctggca ggatcgtcca tgacagtaaa aacggaatca tcggcagctg
     1981 cattcagtga cgcaaacatg ccccccgcca acataacgat ggcaggaact gtcttcaaaa
     2041 gcttcattta tcaagagtcc gtacaacaaa aaaagagacc atcgcggtcc cggaaacttt
     2101 cttaaggatc aaagattagc gtccctggaa aggtaacgaa ttataaaaag gcgcgaataa
     2161 cttagcaatg tattcttatt tcattttttg aataagcatg tggcgaaaac agatttttat
     2221 ttatatatat ttatctgcaa aattttaaat aaagctctaa taaatcatat tgttaatttc
     2281 ttcactttcc gctgattcgg tgccagactg aaatcagcct ataggaggaa atgatggtac
     2341 gtatctatac gttgacactt gcgccctctc tcgatagcgc aacaattacc ccgcaaattt
     2401 atcccgaagg aaaactgcgc tgtaccgcac cggtgttcga acccgggggc ggcggcatca
     2461 acgtcgcccg cgccattgcc catcttggag gcagtgccac agcgatcttc ccggcgggtg
     2521 gcgcgaccgg cgaacacctg gtttcactgt tggcggatga aaatgtcccc gtcgctactg
     2581 tagaagccaa agactggacc cggcagaatt tacacgtaca tgtggaagca agcggtgaac
     2641 agtatcgttt tgttatgcca ggcgcggcat taaatgaaga tgagtttcgc cagcttgaag
     2701 agcaagttct ggaaattgaa tccggggcca tcctggtcat aagcggaagc ctgccgccag
     2761 gtgtgaagct ggaaaaatta acccaactga tttccgctgc gcaaaaccaa gggatccgct
     2821 gcatcatcga cagttctggc gaagcgttaa gtgcagcact ggcgattggt aacatcgagt
     2881 tggttaagcc taaccaaaaa gaactcagtg cgctggtgaa tcgcgaactc acccagccag
     2941 acgatgtccg caaagccgcg caggaaatcg ttaatagcgg caaggccaaa cgggttgtcg
     3001 tttcccttgg tccacaagga gcgctgggtg tcgatagtga aaactgcatt caggtggtgc
     3061 caccaccggt gaaaagccag agtaccgttg gcgctggtga cagcatggtc ggcgcgatga
     3121 cactgaaact ggcagaaaat gcctctcttg aagagatggt ccgttttggc gtagctgcgg
     3181 ggagtgcagc cacactcaat cagggaacac gtctgtgctc ccatgacgat acgcaaaaaa
     3241 tttacgctta cctttcccgc taacaaaaac attcccccag cattggggga atcaccacca
     3301 acctgtcggc aacgcgtttc tccgactatg ctcaaaagtc atgtgataac aaaggggtga
     3361 actatggcca gtggcgatct tgtccattat gtcataaccg tcatgttgca tgaggataca
     3421 ttgactgaaa ttaacgagtt gaataattac ctgactcgcg acggtttttt gctcaccatg
     3481 acggatgatg agggaaatat ccatgagctg gggactaaca cttttggact tatcagtacc
     3541 caaagtgaag aagaaattag agaactggtt tcggggctta cccaaagtgc aaccggcaaa
     3601 gatcctgaaa tcaccatcac gacctgggag gaatggaata gcaacagaaa ataaatggtt
     3661 tttgggcaat aatcagtctg tggtgtgcgt tagctcgtgt ttttacaccg cattcttgcg
     3721 ctaaccttat gatctggcag acaacatggg agagacatca tgtggcaggc aatcagtcgt
     3781 cttttgagcg agcagttagg tgaaggcgaa atcgaactgc gtaatgaact gcctggcgga
     3841 gaagtccatg ccgcatggca tttgcgctat gcaggacatg actttttcgt caaatgtgat
     3901 gaaagggaac tgcttcccgg ttttaccgcc gaagccgacc aactggagtt actgtcgcgt
     3961 agtaaaaccg tcaccgtgcc taaggtttgg gcagtaggcg ctgaccgtga ctacagtttt
     4021 ctggtgatgg attatctccc acctcgtccg ctggatgcgc atagcgcatt tattcttggt
     4081 cagcaaattg cgcgtttaca tcaatggagt gaccaaccac aatttggcct cgattttgat
     4141 aactcgctct ccacaactcc acagcccaac acctggcaac gtcgctggtc aacgtttttt
     4201 gctgaacaac ggattggctg gcagttggaa ctggcagcag agaaagggat cgctttcggc
     4261 aatatcgacg ccatcgtcga gcatattcag cagcgtctgg cctcgcatca accgcagcct
     4321 tctctgttgc acggcgattt atggtccggc aactgtgcgc tgggtccgga tggcccgtac
     4381 attttcgacc ctgcctgcga ctggggtgac cgagagtgcg acctagcgat gttaccgctg
     4441 catactgaac agccgccaca aatctatgac ggctatcagt cagtatcccc gctacctgcc
     4501 gatttccttg aacgtcaacc ggtttaccaa ctctacacgc tgttaaatcg tgcaagatta
     4561 tttggcggtc agcatttggt tattgctcag cagtcattgg atagattatt agcagcatga
     4621 tatgggttga ggataatggc cgctccgtgc ggccttttga ttaaataaac ccaagcagag
     4681 aaaagaacac ataacctgca gcaatacaaa taaccggcag gatatatagt gagaaaaact
     4741 gcaggaaaat agtatggtgc ggaacaacaa tacgggactc aatttgttca cgcgttagcc
     4801 cctcttcccc tttggctttt tccagaatga gttgatcttc aacaccttcc cgcaagaagc
     4861 gcgcctggcg actcatccgg gcaccggaat cctgcaacgc cagtccgaca aaaatcagga
     4921 tgaaaatcac ccagaacata acgttcacac caccattaaa attaggggtc ggggagttat
     4981 accagaacag attcaaaaaa ggcgtattcg cctgcatcat atcgatcatg acatgagcaa
     5041 agtcgagcat gaccgcatta atgccttcct ggttttcctg ccgggtattc ataaatttca
     5101 gcaaagaaat caatgtagag atcaatgcag gtataaaaat cacccaaccc aaaatccttt
     5161 tcaaaacagc aatgcgtcca gcttgttgat acgtcatgag ttctccttga ttaagacgcg
     5221 tcgtttcact tagtttacct gtagatatct gttttcgccc attctttaaa ggcgatatga
     5281 taggcgctta atcataagca cggcttaata ccttacacat aatgctctaa aggagaggtt
     5341 gtaatgtcaa ccccgcgtca gattcttgct gcaatttttg atatggatgg attacttatc
     5401 gactcagaac ctttatggga tcgagccgaa ctggatgtga tggcaagcct gggggtagat
     5461 atctcccgtc gtaacgagct gccggacacc ttaggtttac gcatcgatat ggtggtcgat
     5521 ctttggtacg cccggcaacc gtggaatggg ccaagccgtc aggaagtagt agaacgggtt
     5581 attgcccgtg ccatttcact ggttgaagag acacgtccat tattaccagg cgtgcgcgaa
     5641 gctgttgcgt tatgcaaaga acaaggttta ttggtgggac tggcctccgc gtcaccacta
     5701 catatgctgg aaaaagtgtt gaccatgttt gacttacgcg acagtttcga tgccctcgcc
     5761 tcggctgaaa aactgcctta cagcaagccg catccgcaag tatatctcga ctgcgcagca
     5821 aaactgggcg ttgacccact gacctgcgtg gcgctggaag actcggtaaa tggcatgatc
     5881 gcttctaaag cagcccgcat gcgttccatc gtcgttcctg cgccagaagc gcaaaatgat
     5941 ccacgttttg tattagcaaa cgtcaaactc tcatcgctga cagaactcac cgcaaaagac
     6001 cttctcggtt aatgaccagg ggcagtgatc acgctgcccc tggttcttta tctgaatttg
     6061 cattcaactg acggattaat cgtcaattta agagaaagag ttacaccgtc accacttccg
     6121 tgcactgtat aaaaatccta tactgtacgt atcgacagtt tagtgagttt tatcatgacg
     6181 gcggaaggtc accttctctt ttctattgct tgtgcggtat ttgccaaaaa tgccgagctg
     6241 acgcccgtgc tggcacaggg tgactggtgg catattgtcc cttccgcaat cctgacgtgt
     6301 ttgttaccgg acatcgatca cccaaagtcg tttcttgggc agcgattaaa atggatatca
     6361 aaaccgatcg cccgcgcttt tgggcatcgt ggttttaccc acagtctgct ggcggtattt
     6421 gcgctgctgg caacctttta ccttaaggtt ccggaaggct ggttcattcc ggctgatgcg
     6481 ctacaaggaa tggtgctggg ttatttgagc cacatacttg ccgatatgct gacacccgcc
     6541 ggtgttcccc tgctctggcc atgccgctgg cgtttccgct tgcctatcct ggttccccaa
     6601 aagggcaacc aactggaacg ttttatctgc atggcattat ttgtctggtc ggtatggatg
     6661 ccccattcat tacccgagaa cagcgctgtt cgttggtcat cgcaaatgat caataccttg
     6721 cagatccagt ttcatcggct tattaagcat caggttgaat actaaaaagg caaaaatcac
     6781 ctttctggaa taagcaattc catttgaata taagagccag ctcacagttc tgttaatctt
     6841 gcgccaacac tatgactgct acgcagtgat agaaataata agatcaggag aacggggatg
     6901 aactttccat taattgcgaa catcgtggtg ttcgttgtac tgctgtttgc gctggctcag
     6961 acccgccata aacagtggag tctggcgaaa aaagtgctgg tgggtctggt gatgggtgtg
     7021 gtttttggcc ttgccctgca taccatttat ggttctgaca gccaggtact taaagattct
     7081 gtacagtggt ttaacatcgt tggtaacggc tatgttcaac tgctgcaaat gatcgttatg
     7141 ccgttagtct tcgcctctat tctgagcgcg gttgcccgtc tgcataacgc atctcagtta
     7201 ggcaaaatca gttttctgac catcggtacg cttttgttta ccacgctgat tgcggcgctg
     7261 gtcggtgtgc tggtcaccaa cctgtttggt ttgacggctg aaggtctggt tcagggtggt
     7321 gcagaaactg cacgtctgaa cgccattgaa agtaactatg ttggtaaagt ctctgatcag
     7381 agcgttccgc agctggtctt gtcctttatc ccgaaaaacc cgtttgccga tcttaccgga
     7441 gccaatccga cgtcaattat cagcgtggta atttttgccg cattcctcgg cgtagctgca
     7501 ttgaaactgc tgaaggatga tgcgccgaaa ggggaacgcg tcttaaccgc tatcgatacc
     7561 ctacaaagct gggtgatgaa actggttcgc cttgtcatgc agttgacccc ttacggcgtt
     7621 ctggctctaa tgaccaaagt ggttgcaggt tctaacttgc aagacatcat caaactggga
     7681 agtttcgttg tcgcgtccta cctcggtctg ctgattatgt ttgctgtgca tggcattctg
     7741 ctgggcatta atggcgtgag tccgctgaag tacttccgta aggtatggcc tgtgctgacg
     7801 tttgccttta ccagccgttc cagtgctgc
//
LOCUS       DAJTRC010000151         7782 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.151, whole genome shotgun sequence.
ACCESSION   DAJTRC010000151 DAJTRC010000000
VERSION     DAJTRC010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7782)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7782)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7782
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.151"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            1..1332
                     /gene="yjeM"
                     /locus_tag="OO357_003719"
                     /pseudo
     CDS             1..1332
                     /gene="yjeM"
                     /locus_tag="OO357_003719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418580.2"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate/gamma-aminobutyrate family transporter
                     YjeM"
     gene            complement(1483..4794)
                     /gene="mscM"
                     /locus_tag="OO357_003720"
     CDS             complement(1483..4794)
                     /gene="mscM"
                     /locus_tag="OO357_003720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="miniconductance mechanosensitive channel MscM"
                     /protein_id="HCR5888515.1"
                     /translation="MRLIITFLMAWCLSWGAYAATAPDSKQITQELEQAKAAKPAQPE
                     VVEALQSALNALEERKGSLERIKQYQQVIDNYPKLSATLRAQLNNIRDEPRSVSPGMS
                     TDALNQEILQVSSQLLDKSRQAQQEQERAREIADSLNQLPQQQTDARRQLNEIERRLG
                     TLTGNTPLNQAQNFALQSDSARLKALVDELELAQLSANNRQELARLRSELAEKESQQL
                     DAYLQALRNQLNSQRQLEAERALESTELLAENSADLPKDIVAQFKINRELSAALNQQA
                     QRMELVASQQRQAASQTLQVRQALNTLREQSQWLGSSNLLGEALRAQVARLPEMPKPQ
                     QLDTEMAQLRVQRLRYEDLLNKQPLLRQIHQADGQPLTAEQNRILEAQLRTQRELLNS
                     LLQGGDTLLLELTKLKVSNRQLEDALKEVNEATHRYLFWASDVRPMTIAWPLEIAQDL
                     RRLISLDTFSQLGKASVMMLTSKETILPLFGALILVGCSIYSRRYFTRFLERSAAKVG
                     KVTQDHFWLTLRTLFWSILVASPLPVLWMTLGYGLREAWPYPLAVAIGDGVTATVPLL
                     WVVMICATFARPNGLFIAHFGWPRERVSRGMRYYLMSIGLIMALMMFDNLDDREFSGS
                     LGRLCFILICGALAVVTLSLKKAGIPLYLNKEGSGDNITNHMLWNMMIGAPLVAILAS
                     AVGYLATAQALLARLETSVAIWFLLLVVYHVIRRWMLIQRRRLAFDRAKHRRAEMLAQ
                     RARGEEEAHHHSSPEGAIEVDESEVDLDAISAQSLRLVRSILMLIALLSVIVLWSEIH
                     SAFGFLENISLWDVTSTVQGVESLEPITLGAVLIAILVFIITTQLVRNLPALLELAIL
                     QHLDLTPGTGYAITTITKYLLMLIGGLVGFSMIGIEWSKLQWLVAALGVGLGFGLQEI
                     FANFISGLIILFEKPIRIGDTVTIRDLTGSVTKINTRATTISDWDRKEIIVPNKAFIT
                     EQFINWSLSDSVTRVVLTIPAPADANSEEVTEILLTAARRCSLVIDNPAPEVFLVDLQ
                     QGIQIFELRIYAAEMGHRMPLRHEIHQLILAGFHAHGIDMPFPPFQMRLESLNGKQTG
                     RTLTSAGKGRQAGSL"
     gene            complement(4816..5784)
                     /gene="psd"
                     /locus_tag="OO357_003721"
     CDS             complement(4816..5784)
                     /gene="psd"
                     /locus_tag="OO357_003721"
                     /EC_number="4.1.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313166.1"
                     /note="Phosphatidylserine decarboxylase is synthesized as
                     a single chain precursor. Generation of the pyruvoyl
                     active site from a Ser is coupled to cleavage of a Gly-Ser
                     bond between the larger (beta) and smaller (alpha chains).
                     It is an integral membrane protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase"
                     /protein_id="HCR5888516.1"
                     /translation="MLNSFKLSLQYILPKLWLTRLAGWGASKRAGWLTKLVIDLFVKY
                     YKVDMKEAQKPDTASYRTFNEFFVRPLRDEVRPIDTDPNVLVMPADGVISQLGKIEED
                     KILQAKGHNYSLEALLAGNYLMADLFRNGTFVTTYLSPRDYHRVHMPCNGILREMIYV
                     PGDLFSVNHLTAQNVPNLFARNERVICLFDTEFGPMAQILVGATIVGSIETVWAGTIT
                     PPREGIIKRWTWPAGENDGSVALLKGQEMGRFKLGSTVINLFAPGKVNLVEQLESLSV
                     TKIGQPLAVSTETFVTPDAEPAPLPAEEIEAEHDASPLVDDKKDQV"
     gene            complement(5881..6933)
                     /gene="rsgA"
                     /locus_tag="OO357_003722"
     CDS             complement(5881..6933)
                     /gene="rsgA"
                     /locus_tag="OO357_003722"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313167.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small ribosomal subunit biogenesis GTPase RsgA"
                     /protein_id="HCR5888517.1"
                     /translation="MSKNKLSKGQQRRVNANHQRRLKTSKEKPDYDDNLFGEPDEGIV
                     ISRFGMHADVESADGDVHRCNIRRTIRSLVTGDRVVWRPGKPAAEGVNVKGIVEAVHE
                     RTSVLTRPDFYDGVKPIAANIDQIVIVSAILPELSLNIIDRYLVACETLQIEPIIVLN
                     KIDLLDDEGMAFVNEQMDIYRNIGYRVLMVSSHTQDGLKPLEEALTGRISIFAGQSGV
                     GKSSLLNALLGLQKEILTNDVSDNSGLGQHTTTAARLYHFPHGGDVIDSPGVREFGLW
                     HLEPEQITQGFVEFHDYLGLCKYRDCKHDTDPGCAIREAVEEGKIAETRFENYHRILE
                     SMAQVKTRKNFSDTDD"
     gene            7028..7573
                     /gene="orn"
                     /locus_tag="OO357_003723"
     CDS             7028..7573
                     /gene="orn"
                     /locus_tag="OO357_003723"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoribonuclease"
                     /protein_id="HCR5888518.1"
                     /translation="MSANENNLIWIDLEMTGLDPERDRIIEIATLVTDANLNILAEGP
                     TIAVHQSDEQLALMDDWNVRTHTASGLVERVKASTMGDREAELATLEFLKQWVPAGKS
                     PICGNSIGQDRRFLFKYMPELEVYFHYRYLDVSTLKELARRWKPEILDGFTKQGTHQA
                     MDDIRESVAELAYYREHFIKL"
BASE COUNT         1762 a         2018 c         2104 g         1898 t
ORIGIN      
        1 cttaatgatg gctgaaatgg gagctgctta tcgcaaagaa gaaggtggta tctattcctg
       61 gatgaataat agtgtcggac cacgttttgc cttcattggt acgtttatgt ggttttcctc
      121 ttatatcatc tggatggtga gtacctccgc gaaagtttgg gtaccgttct caacattcct
      181 ctatggtagt gacatgaccc agcactggcg tattgccgga ctggagccta cgcaggtggt
      241 tggtctgctg gcagtagcat ggatgattct ggtcacagtc gttgcttcaa aggggattaa
      301 taaaattgcc cgcattactg cggtgggcgg tattgcagta atgtgtctga atttagtatt
      361 gctgttagta agcattacta ttttgttatt aaatggtggg catttcgcgc aggatattaa
      421 tttccttgca tcaccgaacc cgggttatca gtccggtctg gcaatgctat cgtttgtggt
      481 atttgctatt tttgcctatg gcggaattga agcggttggt ggtctggtcg ataaaacgga
      541 aaatccagaa aagaactttg ccaaaggtat tgtttttgcc gctattgtta tttcaatcgg
      601 ttattcgctg gcaatatttt tatggggcgt cagcacaaac tggcagcagg tattaagtaa
      661 tggttccgtt aaccttggca atattaccta tgtgctgatg aagagcctcg gggtgatgct
      721 gggtaacgca ctgcatttgt cacctgaagc gtcattgtcg ctgggcgtat ggtttgcgcg
      781 tattaccgga ctttcgatgt tcctcgctta taccggtgcg tttgctattc accgctgaaa
      841 gccatcatcc aggggacgcc gaaagcgttg tggccggaac cgatgacgcg cctgaatgcg
      901 atggggatgc cgtctatcgc catgtggatg cagtgcgggt tggttactgt cttcattctg
      961 ctggtttcgt ttggtggcgg taccgcatcg gcgttcttta acaagctgac gctgatggcg
     1021 aacgtgtcta tgacgcttcc ttacctgttc ctcgcgctgg ctttcccgtt ttttaaagca
     1081 cgtcaggatc tcgacagacc atttgtgatt ttcaaaacgc gtatgtcggc aatgattgcg
     1141 acggtggttg tcgttctggt ggtgacattt gcgaacgtct tcaccattat tcagcctgtg
     1201 gttgaagccg gagactggga cagcacattg tggatgattg gcggccctgt cttcttctcg
     1261 ctgttagcga tggcgattta ccagaactat tgcagtcgca tggcgaataa acctgagtta
     1321 gctctcgact gatcatatga aatcctgcaa ctcatcgtct ctccggcgat gagttgtcag
     1381 gaagtaatgc gtatggatga tactctccag ggccggtgat aactgaagat gatgttaagc
     1441 tgctaaatga gccacgttga catcatttgg cagcttcatg cattacaaac ttcccgcctg
     1501 acgacctttg cccgcagacg tcagcgttct ccccgtttgt ttaccgttga ggctttccag
     1561 acgcatctgg aagggcggga atggcatatc gataccgtgg gcgtggaagc ctgccagaat
     1621 cagctggtgg atctcatggc gtagcggcat acggtgaccc atctcagcgg cgtaaatacg
     1681 cagctcgaaa atctgaatcc cctgttgcag atccaccagg aagacttccg gtgccgggtt
     1741 gtcgatcacc agcgagcagc gacgcgctgc ggtgagcagg atttccgtca cttcttcact
     1801 gttggcatcg gcaggggccg gaatcgtcaa cactacgcgc gtgaccgagt cagagagcga
     1861 ccagttgata aactgctcgg taataaacgc cttgttcggc acgattatct ctttacggtc
     1921 ccagtcgctg atggtggtgg cgcgggtgtt aatttttgtc acgctaccag taagatcgcg
     1981 gatcgttacc gtatcgccaa tgcgaatcgg tttttcgaac aggatgatca ggccagagat
     2041 aaagttggcg aaaatttcct gcaaaccaaa accgagacca acaccgagcg cggcaaccag
     2101 ccactgcaat ttcgaccact caataccaat cattgagaag ccgaccagcc cgccaatcag
     2161 catcagcaga tattttgtga tggtggtgat ggcgtaaccc gtacccggcg ttaaatccag
     2221 gtgctgcaaa atcgccagtt ccagcagcgc gggcaggttg cgcaccagct gcgtggtgat
     2281 gataaatacc agaatggcaa tcagcaccgc accgagggta attggctcca gactttctac
     2341 gccctgtacc gtggaggtga catcccacag cgaaatattt tcgaggaagc cgaaagcgga
     2401 atggatttct gaccacagca caatgaccga aagcagggcg atcaacatca aaattgagcg
     2461 caccagccgc aaggattgcg cactgatggc atcgagatcg acttcgcttt catcgacttc
     2521 aattgctcct tccgggctac tgtgatgatg cgcttcctct tcgccacgcg cacgttgcgc
     2581 taacatctct gcccggcgat gcttcgcccg gtcaaacgcc agcctgcgac gctggatcag
     2641 catccagcgg cggataacgt gataaaccac cagtagcagg aaccagatgg caaccgaggt
     2701 ttcaagcctc gctaacagtg cctgcgccgt tgccagataa cccaccgccg acgccagaat
     2761 ggcaaccagt ggtgcgccaa tcatcatgtt ccacagcata tggttggtaa tgttgtcgcc
     2821 gctgccttct ttgttgagat acaacggaat accggctttt ttcaggctta gggtgaccac
     2881 cgccagcgca ccgcaaatga ggataaagca aagccgtccc agcgaaccgg aaaattcgcg
     2941 gtcgtcgagg ttatcgaaca tcatcagcgc cataatcagc ccgatgctca tcaggtagta
     3001 gcgcatccca cgggaaacac gttcgcgcgg ccagccaaaa tgagcgataa acaagccgtt
     3061 cgggcgggca aaggtggcgc aaatcatcac tacccacagc agcggcacgg tggccgttac
     3121 accatcgcca atcgcgaccg ccagcggata aggccacgcc tcgcgcaagc cgtaacccag
     3181 cgtcatccac agcaccggta acggtgacgc gacgagaatc gaccagaaaa gagtgcgcaa
     3241 cgtcagccag aagtgatcct gagtcacttt gccgactttc gccgccgaac gttcaagaaa
     3301 acgggtgaaa tagcggcgcg agtaaatact gcaaccgacc agaatcaacg cgccaaacag
     3361 cggcaaaatt gtctctttgc tggtcagcat catcacactg gctttgccca actgactgaa
     3421 ggtgtccagc gaaatgagac gacgcagatc ctgggcgatt tccagcggcc aggcgatggt
     3481 catcgggcgc acgtcagagg cccagaacag atagcggtgc gttgcttcgt tcacctcttt
     3541 cagcgcatcc tccagttgcc tgttggagac tttcagcttg gtcagttcca atagtagcgt
     3601 gtcgccaccc tgcaacaatg agttcagcaa ctcacgctga gtgcgcagtt gtgcttccag
     3661 aatacggttt tgctcggcgg tcagcggctg accgtcggcc tgatggattt gccgtagcag
     3721 cggctgttta ttaagtaaat cctcataacg taaccgttgc acacgcaact gcgccatttc
     3781 ggtatcaagc tgttgtggtt ttggcatttc tggtagccgt gccacctgcg cccgcagcgc
     3841 ttcgccgagc aggttggacg atcccagcca ttgcgactgt tcacgcagcg tattcaacgc
     3901 ctgccggacc tgtaacgtct ggctggcagc ctgacgctgt tgcgaggcaa cgagctccat
     3961 ccgctgcgcc tgttgattca gcgctgccga tagttcgcgg ttaattttga attgcgcgac
     4021 gatatctttc ggcaaatcgg cactgttttc tgccagtaat tcggtacttt ccagcgcccg
     4081 ctccgcctca agctgacgtt ggctattcaa ctgattacgt aaggcctgca aatacgcatc
     4141 cagttgctgg ctctcttttt ccgccagctc tgagcgtaag cgcgctaatt cctggcggtt
     4201 attggcagac agctgcgcca gctccagttc atcaacgagc gccttaagac gtgcagagtc
     4261 agactgcaac gcgaaatttt gtgcctgatt gagcggagta ttgccggtaa gcgttcccag
     4321 gcggcgctcg atctcattta actgacggcg ggcgtcggtt tgctgttgcg gcagttgatt
     4381 cagcgaatcg gcaatctcgc gggcgcgctc ctgctcttgc tgggcctgac ggcttttatc
     4441 cagcaactgg ctgctgacct ggagaatttc ctgattcagc gcgtcggtag acattcccgg
     4501 cgacacgctg cgcggctcgt cacgaatgtt gtttaattgt gcgcgcagag tagcggagag
     4561 tttcggataa ttatcgataa cttgctgata ttgtttgatg cgctcaaggg aaccttttcg
     4621 ttcctcaagc gcatttaagg cagactggag cgcctctacg acttccggct gtgcgggttt
     4681 cgccgctttt gcctgctcca gttcctgagt gatttgtttg ctatcggggg ccgtcgcggc
     4741 gtacgccccc caactgaggc accaggccat cagaaaagtg ataatcaggc gcacgtcagc
     4801 gtttcctttg atggattaga cctggtcttt tttatcgtca accaatgggc tggcgtcgtg
     4861 ttctgcttcg atctcttcag caggaagtgg ggcgggttca gcgtctggcg taacaaaggt
     4921 ttcggtagat actgccagcg gctggccaat tttcgtgaca gacaggcttt ccagttgctc
     4981 aaccagattc actttacccg gtgcaaacag gttgataacg gtggaaccga gtttaaagcg
     5041 acccatttcc tggcctttca gcagtgccac agaaccgtcg ttttccccgg caggccaggt
     5101 ccagcgcttg atgatacctt cgcgcggcgg cgtaatggtg cccgcccaga ccgtctcaat
     5161 gctgccaaca atcgtcgctc cgaccagaat ctgcgccatt gggccaaatt cggtatcgaa
     5221 aaggcaaatc acgcgttcgt tacgggcaaa cagatttggc acgttctgag cggtgagatg
     5281 gttaacggaa aagagatcgc ccggcacgta gatcatctca cgcagaatac cgttgcacgg
     5341 catgtgtacg cgatggtagt cacgcgggga gaggtaagtg gtcacaaacg taccgttgcg
     5401 gaacaggtcc gccatcagat agttgcctgc cagcagggct tcgaggctgt agttgtggcc
     5461 tttggcttgc aggattttat cttcttcgat tttacccaac tggctgataa cgccatcggc
     5521 aggcatgacc agtacattcg gatcggtatc gattgggcgt acttcgtcac gcagcggacg
     5581 gacaaagaat tcgttaaagg tgcggtagct ggcggtgtcc ggcttttgcg cctctttcat
     5641 gtcgaccttg tagtatttaa cgaacagatc gataaccagt tttgtcagcc atcctgcccg
     5701 cttgcttgcg ccccaacccg ccaggcgagt aagccatagt ttcggcagaa tgtactgtag
     5761 cgaaagttta aatgaattta acaaggtagc ctccaggcca ttgttttgtc gttcctgatc
     5821 cggccaacat gccggatcct gaaaaaaagg ggacgattct aacgacggtt agcttaattg
     5881 tcagtcatcc gtatcagaaa agtttttacg cgtttttacc tgcgccatgc tttccagaat
     5941 acggtgatag ttttcgaaac gggtttccgc gattttccct tcctcaaccg cttcccggat
     6001 agcgcagccc ggatcggtat cgtgtttgca atcgcgatat ttacacagac ctaaatagtc
     6061 atggaattcg acaaagccct gagtgatttg ttccggctcc aggtgccaga ggccgaactc
     6121 acgcacacca ggggaatcaa tcacatcacc gccgtgcggg aagtgataca gccgagcggc
     6181 ggtcgtggtg tgctgaccga gccccgagtt gtcagagaca tcgttggtca ggatctcttt
     6241 ttgcagcccc agcaacgcgt tcagcaggct ggatttaccg acgccagact gcccggcaaa
     6301 aatgctgatg cgcccggtca acgcctcttc cagcggtttc agcccatcct gagtatggct
     6361 ggaaaccatc aatacgcgat aaccgatgtt gcggtagata tccatctgct cgttgacgaa
     6421 cgccatgcct tcgtcgtcca gcaggtctat cttgttgagc acgataatcg gctcaatctg
     6481 caaggtttcg caggccacca ggtaacggtc gataatattg agcgacagct ccggcaaaat
     6541 ggcggagaca atgacaatct ggtcgatgtt ggcggctatg ggtttcacgc cgtcgtagaa
     6601 atccggacgc gttaacaccg aggtgcgctc atgcaccgct tccacgatcc ctttgacatt
     6661 tacgccttcc gccgccggtt tacccggacg ccagactacg cggtcgccgg ttaccagcga
     6721 acggatggta cggcgaatat tgcagcggtg aacgtcgcca tcggcggatt ccacatcagc
     6781 gtgcatacca aagcggctga tgacgatacc ttcatcaggc tcgccaaaca gattgtcgtc
     6841 gtagtcgggc ttctccttag acgttttaag acgacgctgg tgattggcgt tcacgcggcg
     6901 ctgctggcct ttggagagtt tatttttact caatcgtact gactcctggt cgcccctgat
     6961 gggcaaaaca tctatgatac acgcaattgt ggatcaatat agtcactgtg aatgggtgga
     7021 aaatagcatg agtgccaatg aaaacaacct gatttggatc gatcttgaga tgaccggtct
     7081 ggatcccgag cgcgatcgca ttattgagat tgccacgctg gtgaccgatg ccaatctgaa
     7141 tattctggca gaagggccga ccattgcagt acatcagtcc gatgaacagc tggcgctgat
     7201 ggatgactgg aacgtgcgca cccataccgc cagcgggctg gtagagcgcg tgaaagcgag
     7261 cacgatgggc gatcgggaag ctgaactggc aacgctcgaa ttcttaaaac agtgggtgcc
     7321 tgcgggaaaa tcgccgattt gcggtaacag catcggacag gaccgtcgtt tcctgtttaa
     7381 atacatgccg gagctggaag tctacttcca ctaccgttat ctcgatgtca gcaccctgaa
     7441 agagctggcg cgccgctgga agccggaaat tctggatggt tttaccaagc aggggacgca
     7501 tcaggcgatg gatgatatcc gtgaatcggt ggcggagctg gcttactacc gcgagcattt
     7561 tatcaagctg taaaattttt cctggtcacg taagcgccgg aatccacttg cggcggcgtg
     7621 attttgacgc taaatcgaac gtcttgctga taatttcagc acttgagata aaaacgcaaa
     7681 aaaaactttt ttggggggtt gcagagggaa agatttctcg tataatgcgc ctcccgtaac
     7741 gacgcagaaa tgcgaaaatt acgaaagcaa aattaagtag ta
//
LOCUS       DAJTRC010000152         7744 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.152, whole genome shotgun sequence.
ACCESSION   DAJTRC010000152 DAJTRC010000000
VERSION     DAJTRC010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7744)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7744)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7744
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.152"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..1015
                     /gene="ydfJ"
                     /locus_tag="OO357_003724"
     CDS             <1..1015
                     /gene="ydfJ"
                     /locus_tag="OO357_003724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000582800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MHS family MFS transporter YdfJ"
                     /protein_id="HCR5888519.1"
                     /translation="ILLVTLRIIQGLGAGAEISGAGTMLAEYAPKGKRGIISSFVAMG
                     TNCGTLSATAIWAFMFFILSKEELLAWGWRIPFLASVVVMVFAIWLRMNLKESPVFEK
                     VNDSNQPTAKPAPAGSMFQSKSFWLATGLRFGQAGNSGLIQTFLAGYLVQTLLFNKAI
                     PTDALMISSILGFMTIPFLGWLSDKIGRRIPYIIMNTSAIVLAWPMLSIIVDKSYAPS
                     TIMVALIVIHNCAVLGLFALENITMAEMFGCKNRFTRMAISKEIGGLIASGFGPILAG
                     IFCTMTESWYPIAIMIMAYSVIGLISALKMPEVKDRDLSALEDAAEDQPRVVRAAQPS
                     RSL"
     gene            1104..2564
                     /locus_tag="OO357_003725"
     CDS             1104..2564
                     /locus_tag="OO357_003725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HCR5888520.1"
                     /translation="MGNNLLSAKATLPVYDRNNLAPRIVHLGFGAFHRAHQGVYADIL
                     ATEHFSDWGYYEVNLIGGEQQIADLQQQDNLYTVAEMSADAWTARVVGVVKKALHVQM
                     DGLETVLAAMCEPQIAIVSLTITEKGYFHSPATGQLMLEHPMVAADVQNPHQPKTATG
                     VIVEALARRKAAGLPAFTVMSCDNMPENGHVMRDVVTSYAQAVDVKLAQWIEDNVTFP
                     STMVDRIVPAVTEDTLAKIEQLTGVRDPAGVACEPFRQWVIEDNFVAGRPEWEKAGAE
                     LVSDVLPYEEMKLRMLNGSHSFLAYLGYLAGYQHINDCMEDEHYRHAAYGMMLQEQAP
                     TLKVQGVDLQDYANRLIARYSNPALRHRTWQIAMDGSQKLPQRMLDSVRWHLAHDSKF
                     DLLALGVAGWMRYVGGVDEQGNPIEISDPLLPVIQKAVQSSAEGKARVQSLLAIKAIF
                     GDDLPDNSLFTAKVTEAYLSLLAHGAKATVAKYSVK"
     gene            complement(2600..2803)
                     /gene="ydfZ"
                     /locus_tag="OO357_003726"
     CDS             complement(2600..2803)
                     /gene="ydfZ"
                     /locus_tag="OO357_003726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative selenium delivery protein YdfZ"
                     /protein_id="HCR5888521.1"
                     /translation="MTTYDRNRNAITTGSRVMVSGTGHTGKILSIDTEGLTAEQIRRG
                     KTVVVEGCEEKLAPLDLIRLGMN"
     gene            complement(2981..3667)
                     /gene="ydfH"
                     /locus_tag="OO357_003727"
     CDS             complement(2981..3667)
                     /gene="ydfH"
                     /locus_tag="OO357_003727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator YdfH"
                     /protein_id="HCR5888522.1"
                     /translation="MTVETQLNPTQPVNQQIYRILRRDIVHCLIAPGTPLSEKEVSVR
                     FNVSRQPVREAFIKLAENGLIQIRPQRGSYVNKISMAQVRNGSFIRQAIECAVARRAA
                     SMITESQCYQLEQNLHQQRIAIERKQLDDFFELDDNFHQLLTQIADCQLAWDTIENLK
                     ATVDRVRYMSFDHVSPPEMLLRQHLDIFSALQKRDGDAVERAMTQHLQEISESVRQIR
                     QENSDWFSEE"
     gene            complement(3756..4502)
                     /gene="ydfG"
                     /locus_tag="OO357_003728"
     CDS             complement(3756..4502)
                     /gene="ydfG"
                     /locus_tag="OO357_003728"
                     /EC_number="1.1.1.298"
                     /EC_number="1.1.1.381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707438.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional NADP-dependent 3-hydroxy acid
                     dehydrogenase/3-hydroxypropionate dehydrogenase YdfG"
                     /protein_id="HCR5888523.1"
                     /translation="MIVLVTGATAGFGECITRRFIQQGHKVIATGRRQERLQELTDEL
                     GDNLYIAQLDVRNRAAIEEMLASLPAEWSNIDILVNNAGLALGMEPAHKASVEDWETM
                     IDTNNKGLVYMTRAVLPGMVERNHGHIINIGSTAGSWPYAGGNVYGATKAFVRQFSLN
                     LRTDLHGTAVRVTDIEPGLVGGTEFSNVRFKGDDGKAEKTYQNTVALTPEDVSEAVWW
                     VSTLPAHVNINTLEMMPVTQSYAGLNVHRQ"
     gene            4639..6684
                     /gene="dcp"
                     /locus_tag="OO357_003729"
     CDS             4639..6684
                     /gene="dcp"
                     /locus_tag="OO357_003729"
                     /EC_number="3.4.15.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-dipeptidase Dcp"
                     /protein_id="HCR5888524.1"
                     /translation="MTTMNPFLVQSTLPYLAPHFDQIANHHYRPAFDEGMQQKRAEIA
                     AIALNPQTPDFNNTILALEQSGELITRVTSVFFAMTAAHTNDELQRLDEQFSAELAEL
                     ANDIYLNGELFARVDAVWQRRESLGLDSESIRLVEVIHQRFVLAGAKLAQADKAKLKV
                     LNTEAATLTSQFNQRLLAANKSGGLVVNDIAQLAGMSEQEIALAAEAAREKGLDNKWL
                     IPLLNTTQQPALAEMRDRATREKLFIAGWTRAEKNDANDTRAIIQRLVEIRAQQAKLL
                     GFPHYAAWKIADQMAKTPEAALNFMREIVPAARQRASDELASIQAVIDKQQGGFSAQP
                     WDWAFYAEQVRREKFDLDEAQLKPYFELNTVLNEGVFWTANQLFGIKFVERFDIPVYH
                     PDVRVWEIFDHNGVGLALFYGDFFARDSKSGGAWMGNFVEQSTLNETHPVIYNVCNYQ
                     KPAAGEPALLLWDDVITLFHEFGHTLHGLFARQRYATLSGTNTPRDFVEFPSQINEHW
                     ATHPQVFARYARHYQSGAAMPDELQQKMRNASLFNKGYEMSELLSAALLDMRWHCLEE
                     NEAMQDVDDFELRALVAENMDLPAIPPRYRSSYFAHIFGGGYAAGYYAYLWTQMLADD
                     GYQWFVEQGGLTRENGQRFREAILSRGNSEDLERLHRQWRGKAPQIMPMLQHRGLNI"
     gene            complement(6729..7247)
                     /locus_tag="OO357_003730"
     CDS             complement(6729..7247)
                     /locus_tag="OO357_003730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-tetronate isomerase"
                     /protein_id="HCR5888525.1"
                     /translation="MNINKDKIVQLADTDTIENLTSALSQRLIADQLRLTTAESCTGG
                     KLASALCAAEDTPKFYGAGFVTFTDQAKMKILSVSQQSLERYSAVSEKVAAEMATGAI
                     ERADADVSIAITGYGGPEGGEDGTPAGTVWFAWHIKGQNYTAVMHFAGDCETVLALAV
                     RFALAQLLQLLL"
     gene            7523..>7744
                     /gene="ydeI"
                     /locus_tag="OO357_003731"
     CDS             7523..>7744
                     /gene="ydeI"
                     /locus_tag="OO357_003731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI family stress tolerance OB fold protein"
                     /protein_id="HCR5888526.1"
                     /translation="MKFQSIVLASFLVMPYALADDQGGLKQDAAPPPPHAIEDGYRGT
                     DDAKKMTVDFAKNMHDGASVSLRGNLISHK"
BASE COUNT         1941 a         1812 c         1955 g         2036 t
ORIGIN      
        1 catcttgctg gtgacgctgc gtattattca gggattgggt gcaggtgcgg aaatttccgg
       61 tgccggtacg atgctggcgg aatatgcgcc aaaaggtaag cgcggaatta tctcctcatt
      121 tgtggctatg ggaactaact gcggcacctt aagcgcaacg gcaatctggg cctttatgtt
      181 cttcattctc agtaaagagg aactgctggc gtggggatgg cgtataccgt tcctggcgag
      241 cgttgtcgtg atggtctttg ctatctggtt gcgtatgaat ctgaaagaaa gcccggtctt
      301 tgagaaggtt aacgacagca accaaccgac agcaaaacct gcacctgctg gtagcatgtt
      361 ccagagcaaa tccttctggc tggcaacagg gctgcgtttt ggtcaggcgg gtaactccgg
      421 tttaattcag actttccttg caggctattt agtacagacg ttattgttta acaaagcaat
      481 tccaacagat gcattgatga tcagttcgat tctcggcttt atgaccattc cgttccttgg
      541 ttggttatcc gataaaattg gtcgccggat cccgtatatt attatgaata cctctgcgat
      601 tgtgctggcg tggccaatgc tttctatcat cgtagataaa agctatgccc cgagcaccat
      661 tatggttgca ctgattgtga ttcataactg tgcggtgctg ggattatttg ctctggaaaa
      721 cattaccatg gcagaaatgt tcggctgtaa aaaccgcttc acccggatgg ctatttcaaa
      781 agaaattggt ggtcttatcg cttccggttt tggtcctatc ctggcgggta ttttctgcac
      841 catgacggaa tcctggtatc cgatcgccat tatgatcatg gcatattcag tgattggttt
      901 aatctctgcg ctgaaaatgc cagaggtgaa agaccgtgat ttaagtgcgc tggaagacgc
      961 tgcggaagat caaccgcgtg ttgtaagagc tgcgcaacct tccagaagtc tgtaaaccct
     1021 caatcccttc tcttaccgga gaggggattt ttattcatat aacaaaacat atagcttgcc
     1081 atatttaaat ttaaggaatt gttatgggaa ataatttgtt atcagcaaaa gcgacactcc
     1141 ctgtttatga tcgtaataac ctggccccaa gaattgttca tttaggcttt ggtgcatttc
     1201 accgtgcgca tcagggcgtg tatgccgata ttcttgctac agaacatttc agtgactggg
     1261 gatattatga ggtcaactta atcggcggcg aacagcaaat tgccgattta caacagcaag
     1321 ataatcttta taccgttgcg gaaatgtcgg ccgatgcgtg gacggctcgc gtcgttggcg
     1381 tcgttaaaaa agccttgcac gtacagatgg atggcttaga aaccgtgttg gctgcgatgt
     1441 gtgaaccgca aatcgcgatt gtctctctga caatcaccga aaaagggtat ttccactctc
     1501 cggcgaccgg acagttaatg ctcgagcacc cgatggtcgc tgccgacgtg caaaatcccc
     1561 accagccgaa aacagcaaca ggggtgattg ttgaggcgct ggctcgccgt aaagcggcag
     1621 gacttcccgc atttaccgtc atgtcatgtg acaacatgcc agaaaacggt catgttatgc
     1681 gtgacgttgt cacttcctac gcgcaagccg ttgatgtaaa actggcacaa tggatcgaag
     1741 ataacgtgac tttcccatca acaatggtgg accgtattgt gcccgcagtg acagaggata
     1801 cgctggcgaa aatcgaacaa cttaccggtg tgcgcgatcc tgctggcgtt gcctgtgaac
     1861 ctttccgcca gtgggttatc gaagataact ttgtagccgg acgtccggaa tgggaaaaag
     1921 cgggagccga actggttagt gatgtgctgc cttatgaaga gatgaagttg cgcatgctca
     1981 acggcagtca ttcattcttg gcatatctgg gttatcttgc tggatatcag cacattaatg
     2041 actgtatgga agatgaacat tatcgtcatg cggcgtatgg catgatgttg caggaacaag
     2101 cgccgacgct gaaagtgcag ggcgttgatt tgcaagatta cgctaaccga ttaattgcac
     2161 gctatagcaa cccggcgtta cgtcatcgaa cctggcagat tgcgatggat ggcagccaga
     2221 aattgccaca gcggatgttg gattctgttc gctggcatct ggcgcatgac agcaagttcg
     2281 atctgctggc gctgggcgtc gcgggttgga tgcgttatgt cggtggtgtt gatgaacagg
     2341 gaaatccgat agaaatcagt gacccactgt tacctgttat tcagaaggct gtacaaagta
     2401 gtgccgaagg gaaagcgcgc gtccagtcat tgctggcgat taaggcaatc tttggtgatg
     2461 atttgccaga caatagcttg tttactgcaa aagtgacgga agcgtacttg tctttattag
     2521 cgcatggcgc gaaagcgacc gtggcgaaat attccgtgaa gtaaaaaaag caatgccgcc
     2581 atcggcggca ttcacacact tagttcatgc cgagacgaat caggtccaga ggtgccagtt
     2641 tctcttcaca accttcaaca actacggttt ttccgcggcg gatttgctca gcggtcagac
     2701 cttcagtatc aatcgacagg atcttgccag tgtgaccggt gccgctaacc ataacacggc
     2761 tgccagtggt gattgcgtta cggttacgat cgtaggtcgt cataggtgtt ttctcctttc
     2821 tgatttacag tcgaagccca cgtaacgggc gcaatataaa tactcctgtc gagtgaaata
     2881 ttttttgttt ttgatcaagc tcacatcttt tttgatgcat gcatgagccg taaggcgggg
     2941 ggaaaagcct ctcatcccgg atgggatgag agtaaatgaa ttactcttcg ctaaaccagt
     3001 cgctgttttc ctggcggatc tggcgcacgg attcgctgat ttcctgcaaa tgttgcgtca
     3061 ttgcacgttc taccgcatcg ccatcacgtt tttgcagggc agagaaaata tcaagatgct
     3121 ggcgtaacag catttctggt ggagaaacat ggtcgaaact catatagcgc acgcgatcaa
     3181 cggtcgcttt caggttctca atggtatccc acgccagttg acagtcggca atctgcgtca
     3241 ggagttgatg gaagttgtca tcaagttcaa aaaaatcatc cagttgcttg cgctcaatgg
     3301 caatgcgttg ctggtgaaga ttttgttcca gttggtagca ctggctttcg gtaatcatgc
     3361 tcgccgcccg acgcgccacc gcgcactcaa tggcctgacg gataaaactg ccgttgcgca
     3421 cctgggccat ggaaattttg ttgacgtagc tgccacgctg cggacgaatt tgaatcaggc
     3481 cgttttccgc cagtttaata aaggcttcac gaaccggctg gcgtgacaca ttgaaacgaa
     3541 cagaaacttc tttttccgac aacggtgtgc ctggtgcaat caggcaatgg acaatgtcgc
     3601 gacgaagaat acgataaatc tgctgattga caggctgtgt gggattaagt tgcgtttcga
     3661 cggtcattcg ttcttgcttg tgagtgagtt aactgcatga gtctaccact tttttgtgac
     3721 aagcaataac ccggcagtta cgccgggtat aaaaattact gacggtggac attcagtccg
     3781 gcatagcttt gggtaaccgg catcatttcc agggtattga tattgacgtg agcaggcagc
     3841 gttgacaccc accagacggc ttcgctgaca tcttctggcg tcaatgcaac ggtattttga
     3901 taggtttttt ccgctttacc gtcatcgcct ttaaagcgga cattggaaaa ctcggtgcca
     3961 cccaccagac ccggttcgat gtcggtgacg cgcaccgccg taccatgcag atccgtacgc
     4021 agattcaggc taaactgacg aacaaacgct ttcgtcgcac cgtaaacgtt accaccggca
     4081 tacggccagc tgcctgccgt tgagccaatg ttaataatat gaccatgatt acgttcaacc
     4141 atacccggta agacggcgcg cgtcatatat actaggcctt tgttgttggt atcaatcatc
     4201 gtttcccagt cttcaacgct ggctttatgc gcaggctcca tgcccaacgc caggccggca
     4261 ttatttacca ggatatcaat attgctccac tcggcaggga gcgatgccag catctcttca
     4321 atagcggcgc ggttgcgaac gtccagttgg gcgatataca gattatctcc cagttcgtct
     4381 gttaactcct gcaaccgctc ctggcgacgg ccagtggcga taactttatg cccttgttga
     4441 ataaaacgac gagtaatgca ttcaccaaaa cctgccgttg ctccagttac taaaacgatc
     4501 atctcactgt tcctcaacgc ttttgtgtgt caataacata gcacgtgatg cttaagagcg
     4561 ggtaatactg gtttgcaaga ttgcactccg ttatcagtaa gcctacgcta tggaacacct
     4621 cgcgttcagg agaagaaaat gacaacaatg aatcctttcc ttgtgcaaag cacactgccg
     4681 tatctggctc ctcattttga tcaaattgcc aatcatcact atcgcccggc attcgatgag
     4741 ggaatgcaac aaaagcgggc agaaattgct gccatcgcgc ttaacccgca aacgcctgat
     4801 ttcaacaata ctattctggc actggaacaa agcggagaat taattacccg cgttaccagc
     4861 gtcttttttg cgatgactgc ggcgcatacc aatgatgaat tacagcgtct tgatgaacag
     4921 ttttccgctg aactggcgga actggctaat gatatctatc tgaacggtga attattcgcg
     4981 cgggtagatg ctgtctggca gcgccgtgaa tccctggggc ttgatagtga atccatccgc
     5041 ctggtggagg ttattcatca acgttttgtc cttgccggag ccaaacttgc acaagctgat
     5101 aaagcaaaat taaaagtact gaatacagaa gctgcgaccc tgaccagcca gtttaaccag
     5161 cggttacttg cagcaaataa atccggcggt ctggttgtga acgatatcgc gcagctggca
     5221 ggaatgagtg agcaagagat tgcgctggcg gcagaggcgg ctcgcgagaa aggtctggat
     5281 aacaaatggc tgattccgct gctgaatacc acccaacaac cggcgcttgc cgaaatgcgc
     5341 gatcgtgcga cgcgtgaaaa actgtttatt gcgggctgga cgcgagcgga aaaaaatgat
     5401 gccaatgata cccgcgctat cattcaacgt ctggtagaga ttcgcgcaca gcaggcgaaa
     5461 ctgcttggtt ttcctcatta tgccgcatgg aaaatcgccg atcagatggc aaaaacgcca
     5521 gaagcagcac tcaactttat gcgggaaatt gttccagcgg cgcgtcaacg tgcgagcgat
     5581 gaattagcct ccatacaggc ggttatcgat aagcagcagg gcgggtttag cgcgcagccg
     5641 tgggactggg cattttatgc ggaacaggta cggcgggaga aatttgatct tgatgaggcg
     5701 cagctcaagc catattttga attaaacacg gtgttaaatg aaggtgtatt ctggaccgcg
     5761 aatcagctct tcggtattaa gtttgtcgaa cgttttgata ttccggtcta ccatcctgac
     5821 gttcgtgtgt gggaaatttt tgatcataat ggcgtgggac tggcgttatt ttacggtgat
     5881 ttcttcgccc gtgattcaaa aagcggcggt gcatggatgg gcaattttgt tgagcaatca
     5941 acgcttaatg aaacgcatcc ggtaatttat aacgtctgca attatcagaa acccgctgcc
     6001 ggtgagcctg cgttgttact ctgggatgat gtaataacct tattccatga atttggtcat
     6061 acgctgcacg gcctttttgc ccgccagcgt tatgccacgc tttccggcac caacacgccg
     6121 cgtgattttg tcgaatttcc gtcgcaaatc aacgaacact gggcaacgca tccgcaggta
     6181 ttcgctcgct acgcccggca ttatcagagc ggggcagcaa tgcctgacga actgcaacag
     6241 aaaatgcgta atgccagcct gttcaacaaa gggtatgaga tgagcgaact gcttagcgcc
     6301 gcacttctcg atatgcgctg gcattgcctg gaagaaaacg aagcaatgca ggatgtcgat
     6361 gattttgaat tgcgggcgct ggtggcggaa aatatggatc ttcctgctat accgccacgc
     6421 tatcgcagca gttatttcgc ccatattttt ggtggcggat atgctgcagg ttattacgct
     6481 tatctgtgga cgcaaatgtt ggccgatgat ggttaccagt ggtttgttga gcagggcgga
     6541 ttaacgcgtg aaaatgggca gcgttttcgc gaggcgatcc tttccagagg taacagcgaa
     6601 gatctggaac gcctgcatcg acaatggcgt ggtaaggctc ctcagattat gccgatgctg
     6661 caacatcgtg gcttgaacat ataaacacca ccaatggctg gcctggagat atcgccaggc
     6721 cagcctggtt atagcagtaa ttgcagcagc tgggcgaggg caaacctcac cgctaaagct
     6781 aataccgttt cgcagtcgcc agcaaaatgc ataaccgcag tgtagttctg gcctttaata
     6841 tgccacgcga accagacggt acccgctggc gtaccatctt caccgccctc cggtccgccg
     6901 tagccggtaa tggcaatact gacatcagca tccgcacgct ctatggcacc tgttgccatt
     6961 tctgctgcca ctttctcact caccgcagaa tatcgttcaa gagactgctg gcttacgctg
     7021 aggattttca tctttgcctg atcggtgaaa gtaacaaagc ctgcaccgta aaatttgggt
     7081 gtatcttcag ctgcacacag ggcgctagcc aacttaccgc cggtgcatga ttcggcggta
     7141 gttaagcgta attgatccgc gataagtctt tgactcaacg cggatgtcag gttttcaata
     7201 gtgtcggtat ctgcgagttg tacaatttta tctttattaa tgttcactgt tttttgtcca
     7261 cgctattata tgtaaaggtg atttgctttt tctgtatgct cgtccaatat aataattttc
     7321 aaacatataa atccgattat tctgaaaaca tgtttaagac tcttcaatct tcttaacttt
     7381 agtgagtcaa attctctgaa tatttgacag ctagtgggct gattttaaat gccttataac
     7441 aattggcagt attcttattt ggcaattata aaaatagcaa ccagacttaa taagtcaaac
     7501 caaacgcaat taaggagtta gtatgaaatt tcagtccatt gtattagcaa gttttcttgt
     7561 catgccttat gcgttggcag atgaccaggg cggtttaaaa caggatgcag cgccaccgcc
     7621 gcctcatgca atagaagatg gctatcgcgg aaccgatgat gcaaaaaaaa tgaccgttga
     7681 tttcgcaaaa aacatgcacg atggcgcttc ggtttcacta cgcggtaatt tgatttctca
     7741 caaa
//
LOCUS       DAJTRC010000153         7539 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.153, whole genome shotgun sequence.
ACCESSION   DAJTRC010000153 DAJTRC010000000
VERSION     DAJTRC010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.153"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            1..1158
                     /gene="rbsA"
                     /locus_tag="OO357_003732"
                     /pseudo
     CDS             1..1158
                     /gene="rbsA"
                     /locus_tag="OO357_003732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418205.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="ribose ABC transporter ATP-binding protein RbsA"
     gene            1163..2128
                     /gene="rbsC"
                     /locus_tag="OO357_003733"
     CDS             1163..2128
                     /gene="rbsC"
                     /locus_tag="OO357_003733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002882531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HCR5888527.1"
                     /translation="MITQTVSGRRYFTKAWLMEQKSLIALLVLIAIVSTLSPNFFTIN
                     NLFNILQQTSVNAIMAVGMTLVILTSGIDLSVGSLLALTGAVAASIVGIEVNALVAVA
                     AALTLGAAIGAVTGVIVAKGRVQAFIATLVMMLLLRGVTMVYTNGSPVNTGFTENADL
                     FGWFGIGRPLGVPTPVWIMGIVFLAAWYMLHHTRLGRYIYALGGNEAATRLSGINVNK
                     IKIIVYSLCGLLASLAGIIEVARLSSAQPTAGTGYELDAIAAVVLGGTSLAGGKGRIV
                     GTLIGALILGFLNNGLNLLGVSSYYQMIVKAVVILLAVLVDNKKQ"
     gene            2153..3043
                     /gene="rbsB"
                     /locus_tag="OO357_003734"
                     /pseudo
     CDS             2153..3043
                     /gene="rbsB"
                     /locus_tag="OO357_003734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312720.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter substrate-binding protein
                     RbsB"
     gene            3169..4098
                     /gene="rbsK"
                     /locus_tag="OO357_003735"
     CDS             3169..4098
                     /gene="rbsK"
                     /locus_tag="OO357_003735"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HCR5888528.1"
                     /translation="MQNAGSLVVLGSINADHILNLQSFPTPGETVTGNHYQVAFGGKG
                     ANQAVAAGRSGANIAFIACTGDDSIGESVRQQLATDNIDITPVSVIKGESTGVALIFV
                     NGEGENVIGIHAGANAALSPSLVEAQRERIANASALLMQLESPLESVMAAAKIAHQNK
                     TIVALNPAPARELPDELLALVDIITPNETEAEKLTGIRVENDEDAAKAAQVLHEKGIR
                     TVLITLGSRGVWASVNGEGQRVPGFRVQAVDTIAAGDTFNGALITALLEEKPLPEAIR
                     FAHAAAAIAVTRKGAQPSVPWREEIDAFLDRQR"
     gene            4102..5094
                     /gene="rbsR"
                     /locus_tag="OO357_003736"
     CDS             4102..5094
                     /gene="rbsR"
                     /locus_tag="OO357_003736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose operon transcriptional repressor RbsR"
                     /protein_id="HCR5888529.1"
                     /translation="MATMKDVARLAGVSTSTVSHVINKDRFVSEAITAKVEAAIKELN
                     YAPSALARSLKLNQTHTIGMLITASTNPFYSELVRGVERSCFERGYSLVLCNTEGDEQ
                     RMNRNLETLMQKRVDGLLLLCTETHQPSREIMQRYPTVPTVMMDWAPFDGDSDLIQDN
                     SLLGGDLATQYLIDKGHTRIACITGPLDKTPARLRLEGYRAAMKRAGLNIPDGYEVTG
                     DFEFNGGFDAMRQLLSHPLRPQAVFTGNDAMAVGVYQALYQAELQVPQDIAVIGYDDI
                     ELASFMTPPLTTIHQPKDELGELAIDVLIHRITQPTLQQQRLQLTPILMERGSA"
     gene            complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="OO357_003737"
     CDS             complement(5060..6487)
                     /gene="mdtD"
                     /locus_tag="OO357_003737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug transporter subunit MdtD"
                     /protein_id="HCR5888530.1"
                     /translation="MSDKKKRSMSGLPWIAAMAFFMQALDATILNTALPAIAHSLNRS
                     PLAMQSAIISYTLTVAMLIPVSGWLADRFGTRRIFTLAVSLFTLGSLACALSNSLPQL
                     VVFRVIQGIGDAMMMPVARPALLRAYPRNELLPVLNFVAMPGLVGPILGPVLGGVLVT
                     WATWHWIFLINIPIGIAGLLYARKHMPNFTTARRRFDITGFLLFGLSLVLFSSGIELF
                     GEKIVASWIALTVIVTSIGLLLLYILHARRTPNPLISLDLFKTRTFSIGIVGNIATRL
                     GTGCVPFLMPLMLQVGFGYQAFIAGCMMAPTALGSIIAKSMVTQVLRRLGYRHTLVGI
                     TVIIGLMIAQFSLQSPAMAIWMLILPLFILGMAMSTQFTAMNTITLADLTDDNASSGN
                     SVLAVTQQLSISLGVAVSAAVLRIYEGMEGTTTVEQFHYTFITMGIITVASAAMFMLL
                     KTTDGNNLIKRQRKSMPNRVPSESE"
     gene            complement(6510..7202)
                     /locus_tag="OO357_003738"
     CDS             complement(6510..7202)
                     /locus_tag="OO357_003738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709569.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FadR family transcriptional regulator"
                     /protein_id="HCR5888531.1"
                     /translation="MPLSAQQLAAQKNLSYVLAEKLAQRILKGEYEPGTILPGEIELG
                     EQFGVSRTAVREAVKTLTAKGMVLPRPRIGTRVMPQSNWNFLDQELLTWWITEENFHQ
                     VIDHFLVMRICLEPQACLLAATVGTAEQKAHLNTLMAEMAALKENFRRERWIEVDMAW
                     HEHIYEMSANPFLTSFASLFHSVYHTYFTSITSDTVIKLDLHQAIVDAIIQSDGDAAF
                     KACQALLRSPDK"
BASE COUNT         1916 a         1830 c         1986 g         1807 t
ORIGIN      
        1 aaccatgtat gtcgaagcgg ataaattgct ggctaaactt aacctgcgct ttaaaagcga
       61 caagttagtg ggcgatcttt ccatcggtga ccagcaaatg gttgaaatcg ccaaagtgct
      121 gagctttgag tcgaaagtca tcattatgga tgaaccgacc gatgcgctga ccgataccga
      181 aaccgaatcc ctgttccgcg tcatccgcga gctgaaatcg caaggccact gtattgtcta
      241 tatctcccac cgcatgaaag aaatcttcga gatttgcgat gacgttaccg tttttcgtga
      301 tgggcaattt attgctgagc gcgaagtggc atcactgacc gaagattcgc tgattgagat
      361 gatggtgggc cgcaagctgg aagatcaata tccacacctg gacaaagcgc caggagatat
      421 ccgcctgaaa gtcgataatc tctgcggacc tggcgttaac gatgtctctt ttactttacg
      481 caaaggcgaa attcttggcg tctctggttt gatgggcgca ggtcgtaccg aactgatgaa
      541 agtgctctac ggcgcactgc cgcgcaccag cggttacgtc accctggatg ggcatgaagt
      601 cgttacccgt tcaccgcagg atggcctggc aaacggcatt gtgtatatct ccgaagaccg
      661 taaacgtgac ggtttagtgc tgggcatgtc agtaaaagag aacatgtcgc tgacagcgct
      721 gcgctacttc agccgcgctg gcggcagttt gaagcatgcc gatgaacagc aggctgtgag
      781 tgatttcatt cgtctgttta atgtgaaaac gccgtcgatg gaacaggcaa ttggtctgct
      841 ttccggtggc aatcagcaaa aagtggcgat tgcccgcggt ctgatgacac ccccaaagta
      901 ttgatccttg atgagcctac ccgtggcgta gatgtcggcg cgaaaaaaga gatctatcaa
      961 ctgattaacc agttcaaagc cgatggcttg agtatcattc tggtgtcatc ggagatgcca
     1021 gaagtattag gcatgagcga tcgcatcatc gtcatgcatg aagggcatct cagcggggaa
     1081 tttactcgtg agcaggccac ccaggaagtg ttaatggctg tcgctgtggg caagcttaat
     1141 cgcgtgaatc aggagtaaaa aaatgataac ccagactgtc tctggtcgcc gttatttcac
     1201 gaaagcgtgg ctgatggagc agaaatcgct tatcgctctg ctggtgctga tcgcgattgt
     1261 ctcgacgtta agcccgaact ttttcaccat caataactta ttcaatattc tccagcaaac
     1321 ttcggtgaac gccattatgg cggtcgggat gacgctggtg atcctgacgt cgggcataga
     1381 cttgtcggta ggttctctgt tggcgctaac cggcgcagtt gctgcatcta tcgtcggcat
     1441 tgaagtcaat gcgctggtgg ctgtcgctgc tgctctcacg ttaggtgccg caattggtgc
     1501 ggtaaccggg gtgattgtag cgaaaggtcg cgtccaggcg tttatcgcta cgctggttat
     1561 gatgctttta ctgcgcggcg tgaccatggt ttataccaac ggtagcccag tgaataccgg
     1621 ctttactgag aacgccgatc tgtttggctg gtttggtatt ggtcgtccgc tgggcgtacc
     1681 gacgccagtc tggatcatgg ggattgtctt cctcgcggcc tggtacatgc tgcatcacac
     1741 gcgtctgggg cgttacatct acgcgctggg cggcaacgaa gcggcaacgc gtctttctgg
     1801 tatcaacgtc aataaaatca aaatcatcgt ctattctctt tgtggtctgc tggcatcgct
     1861 ggccgggatc attgaagtgg cgcgtctctc ctctgcacaa cccacggcgg ggactggcta
     1921 tgagctggat gctattgctg cggtggttct gggcggtacg agtctggcgg gcggaaaagg
     1981 tcgcattgtt gggacgttga tcggcgcatt aattcttggc ttccttaata atggattgaa
     2041 tttgttaggt gtttcctcct attaccagat gatcgtcaaa gcggtggtga ttttgctggc
     2101 ggtgctggta gacaacaaaa agcagtaata acgactacag gacatcttga atatgaacat
     2161 gaaaaaactg gctaccctgg tttccgctgt tgcgctaagc gccaccgtca gtgcgaatgc
     2221 gatggcaaaa gacaccatcg cgctggtggt ctccacgctt aacaacccgt tctttgtatc
     2281 gctgaaagat ggcgcgcaga aagaggcgga taaacttggc tataacctgg tggtgctgga
     2341 ctcccagaac aacccggcga aagagctggc gaacgtgcag gacttaaccg ttcgcggcac
     2401 caaaattctg ctgattaacc cgaccgactc cgacgcagtg ggtaatgctg tgaagatggc
     2461 taactaggcg aacattccgg ttatcactct tgaccgccag gcaacgaaag gtgaagtggt
     2521 gagccacatt gcatctgata acgtactggg cggcaaaatc gccggtgatt acatcgcgaa
     2581 gaaagcgggt gaaggtgcca aagttatcga gctgcaaggc attgccggta catccgcagc
     2641 ccgtgaacgt ggcgaaggct tccagctggc cgttgctgct cacaagttta atgttcttgc
     2701 cagccagcca gcagattttg atcgcaccaa aggtttgaac gtaatgcaga acctgttgac
     2761 cgctcatccg gatgttcagg ctgtattcgc gcagaatgat gaaatggcgc tgggcgcgct
     2821 gcgcgcactg caaactgccg gtaaatcgga tgtgatggtc gtcggatttg acggtacacc
     2881 ggatggcgaa aaagcggtga atgatggcaa actagcagcg actatcgctc agctacccga
     2941 tcagattggc gcgaaaggcg ttgaaaccgc agataaagtg ctgaaaggcg agaaagttca
     3001 ggctaagtat ccggttgatc tgaaactggt tgttaagcag tagttttaat caggttgtat
     3061 gacctgatgg tgacataaat acgtcatcga cagatgaacg tgtaatataa agaaaagcag
     3121 ggcacgcgcc accctaacac ggtggcgcat tttatggaca tcccgaatat gcaaaacgca
     3181 ggcagcctcg ttgttcttgg cagcattaat gctgaccaca ttcttaatct tcaatctttt
     3241 cctactccag gcgaaaccgt aaccggtaac cactatcagg ttgcatttgg cggcaaaggc
     3301 gcgaatcagg ctgtggctgc tgggcgtagc ggtgcgaata tcgcgtttat tgcctgtact
     3361 ggcgatgaca gcattggcga gagcgttcgc cagcagctcg ccactgataa catcgatatt
     3421 actccggtca gcgtgatcaa aggcgaatca acaggtgtgg cgctgatttt tgttaatggc
     3481 gaaggtgaga atgtcatcgg tattcatgcc ggcgctaatg ctgctctttc cccatcgctg
     3541 gtggaagcgc aacgtgagcg tattgccaac gcgtcagcgt tattaatgca gctggaatca
     3601 ccactcgaaa gtgtgatggc agcggcgaaa atcgcccatc aaaataagac tatcgttgcg
     3661 cttaacccgg ctccggctcg cgaacttcct gacgaactgc tggcgctggt ggacattatt
     3721 acgccaaacg aaacggaagc agaaaagctc accggtattc gtgttgaaaa tgatgaagat
     3781 gcagcgaagg cggcgcaggt actgcatgaa aagggtatcc gtactgtact gattacttta
     3841 ggaagtcgtg gtgtatgggc tagcgtgaat ggtgaaggtc agcgcgttcc tggattccgg
     3901 gtgcaggctg tcgataccat tgctgccgga gataccttta acggcgcgtt aatcacggcg
     3961 ttgctggaag aaaaaccatt gccagaggcg attcggtttg cccatgctgc cgctgcgatt
     4021 gccgtaacac gtaaaggcgc acaaccttcc gtaccgtggc gtgaagagat cgacgcattt
     4081 ttagacaggc agaggtgacg cttggctaca atgaaagatg ttgcccgttt ggcgggcgtt
     4141 tctacctcaa cagtttctca cgttatcaat aaagatcgct tcgtcagtga agcgattacc
     4201 gccaaagttg aagcggcgat taaagaactc aattacgcgc catcagctct ggcgcgtagc
     4261 ctcaaactca atcaaacaca taccattggc atgttgatca ctgccagtac caatcctttc
     4321 tattcagaac tggtgcgtgg cgttgaacgc agctgcttcg aacgcggtta tagtctcgtc
     4381 ctttgcaata ccgaaggcga tgaacagcgg atgaatcgca atctggaaac gctgatgcaa
     4441 aaacgcgttg atggcttact gttactgtgt accgaaacgc atcagccttc gcgtgaaatc
     4501 atgcaacgtt atccgacagt gcctaccgtg atgatggact gggctccgtt cgatggcgac
     4561 agcgatctta ttcaggataa ctcgttgctg ggcggagact tagcaacgca atatctgatc
     4621 gataaaggtc atacccgtat cgcctgtatt accggtccgc tggataaaac tccggcgcgc
     4681 ctgcggttgg aaggttatcg ggcggcgatg aaacgtgcgg gtctcaacat tcctgatggc
     4741 tatgaagtca ctggtgattt tgaatttaac ggcgggtttg acgccatgcg ccaactgtta
     4801 tcacatccgc tgcgtcctca ggccgtcttt accggaaatg acgctatggc tgttggcgtt
     4861 taccaggcgt tatatcaggc agagttacag gttccgcagg atatcgcggt gattggctat
     4921 gacgatatcg aactggcaag ctttatgacg ccaccattaa ccactatcca ccaaccgaaa
     4981 gatgaactgg gggagctggc gattgatgta ctcatccatc ggataaccca gccgaccctt
     5041 cagcaacaac gattacaact tactccgatt ctgatggaac gcggttcggc atagatttac
     5101 gctgtctttt gatcaaatta ttaccatcgg ttgttttcag aagcatgaac attgctgctg
     5161 aagcaacagt aataatgccc atcgtgataa acgtatagtg gaattgttcg acagtcgttg
     5221 tgccttccat cccttcataa atgcgaagga cggccgcact tacagcaacg cctaaactaa
     5281 tcgacagttg ctgcgtgacc gccagaacac tgttaccgct gctggcgttg tcatcggtca
     5341 gatcggcaag tgtgatggta ttcatcgcgg taaactgcgt cgacatagcc atccctaata
     5401 taaacaacgg caagatcagc atccatatcg ccattgccgg tgattgcaaa gagaactgag
     5461 cgatcattag cccaataatc accgtgatcc ccactaatgt atggcgatag cccagacgac
     5521 gtaagacttg ggtaaccatc gattttgcaa taatggaacc taacgctgtc ggtgccatca
     5581 tacagccggc aataaacgcc tgataaccaa atcctacctg taacatcaat ggcataagga
     5641 acggtacaca gccggtcccc agacgggttg caatattgcc tacgataccg atcgagaaag
     5701 tgcgggtttt aaataaatct aatgaaatta atgggtttgg cgtacgtcgc gcatggagaa
     5761 tatagagaag cagtaacccg atgctggtga caattaccgt caaggcaatc cagctggcga
     5821 caatcttttc cccgaatagc tctattccgc ttgaaaagag aacaaggctg aggccaaaca
     5881 gcaaaaagcc agtgatatcg aatctgcgtc gtgcggtggt gaaattgggc atatgtttgc
     5941 gcgcgtaaag aaggcccgca atacctatgg ggatattaat taaaaatatc cagtgccagg
     6001 ttgcccaggt gaccagcacg ccgccaagaa cggggcctaa aattggcccc accagacccg
     6061 gcatggcgac aaaattcaat accggaagaa gttcattacg aggataagca cgcagtaagg
     6121 ccggccgagc aacaggcatc atcattgcgt cgcctatccc ttgaataacc cggaagacaa
     6181 ccagctgtgg tagcgaatta gaaagtgcgc aggccagaga acccaacgtg aacagactca
     6241 cggcaagggt aaaaatgcga cgcgtaccga agcgatcggc tagccatccg cttaccggaa
     6301 taagcatcgc caccgtcagc gtataactga tgatggctga ttgcatcgcg agaggagaac
     6361 gattaaggct atgagcgatt gcgggtaagg cggtattcag aatagtggca tcaagtgcct
     6421 gcatgaagaa ggccatcgcc gcgatccacg gcaaacccga catactacgc ttctttttat
     6481 cgctcattca atgtcctgtt atcgggttat cacttatcag gtgagcgtag cagcgcctga
     6541 caagctttaa atgccgcgtc gccatcgctt tggataatcg catcgacaat cgcctggtgc
     6601 agatccagct ttatcactgt gtcgctggta attgacgtga agtaagtgtg ataaaccgaa
     6661 tggaatagcg aggcgaatga ggtcaaaaac ggattggcgc tcatttcata gatatgctca
     6721 tgccaggcca tatcgacttc gatccagcgt tcacggcgaa agttctcttt taatgccgcc
     6781 atttcggcca ttaacgtatt gagatgcgcc ttctgttccg cggtgccaac cgttgctgcc
     6841 agtaggcagg cttgcggctc cagacagata cgcataacca gaaagtgatc gatgacctga
     6901 tgaaagttct cttctgttat ccaccaagta agcaattcct gatcaagaaa attccagttc
     6961 gattgtggca tgacccgagt accaattcgc ggtcgcggta aaaccatccc ttttgccgtt
     7021 aacgttttga ccgcttcgcg taccgctgta cgactcactc caaattgctc gcccagctca
     7081 atctcaccgg gcaaaatggt gccgggttca tattcacctt ttaagatccg ttgcgccagc
     7141 ttctcagcca gaacatacga aaggtttttc tgtgcagcta actgttgtgc gcttaaaggc
     7201 attacttatc ttcctttttc tttttattcc tccttagtat gccaccagga agtgtgatta
     7261 cggttgcaaa aacggcaaat tgcttgtttt atggcacatt aacggggtgt tttgctgaaa
     7321 aaatgcgcgg tcagaaaatt attttaaatt tcctcttgtc aggccgggat aactccctat
     7381 aatacgccac cactgacacg gaacaacggc aaatacgccg ccgggtcagc ggggttctcc
     7441 tgagaattcc gacagaaaaa gcaaaaataa atgattgact ctgtagcggg aaagcgtatt
     7501 atgcacaccc cccccccgcg ccgctgagaa aaagcgaag
//
LOCUS       DAJTRC010000154         7183 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.154, whole genome shotgun sequence.
ACCESSION   DAJTRC010000154 DAJTRC010000000
VERSION     DAJTRC010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7183)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7183)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7183
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.154"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(296..1198)
                     /locus_tag="OO357_003739"
     CDS             complement(296..1198)
                     /locus_tag="OO357_003739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HCR5888532.1"
                     /translation="MARLFTPSESKYYLMALDAGTGSIRAVIFDLEGNQIAVGQAEWR
                     HLAVPDVPGSMEFDLNKNWQLACECMRQALHNAGIDPEYIAAVSACSMREGIVLYNNE
                     GTPIWACANVDARAAREVSELKELHNNTFENEVYRATGQTLALSAIPRLLWLAHHRSD
                     IYRQASTITMISDWLAYMLSGELAVDPSNAGTTGLLDLTTRAWKPALLDMAGLRADIL
                     SPVKETGTLLGVVSSQAAELCGLKAGTLVVVGGGDVQLGCLGLGVVRPAQTAVLGGTF
                     TDNKQCTLSEARHQLPITPFRMPS"
     gene            complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OO357_003740"
     CDS             complement(1277..2230)
                     /gene="lsrR"
                     /locus_tag="OO357_003740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator LsrR"
                     /protein_id="HCR5888533.1"
                     /translation="MTINDSAISEQGMCEEEQVARIAWFYYHDGLTQSEISDRLGLTR
                     LKVSRLLEKGHQSGIIRVQINSRFEGCLEYETQLRRQFSLQHVRVIPGLADADVGGRL
                     GIGAAHMLMSLLQPQQMLAIGFGEATMNTLQRLSGFISSQQIRLVTLSGGVGSYMTGI
                     GQLNAACSVNIIPAPLRASSADIARTLKNENCVKDVLLAAQAADVAIVGIGAVSQQDD
                     ATIIRSGYISQGEQLMIGRKGAVGDILGYFFDAKGDVVTDIKIHNELIGLPLSALKTI
                     PVRVGVAGGENKAEAIAAAMKGGYINALVTDQDTAAAILRS"
     gene            2479..4014
                     /gene="lsrA"
                     /locus_tag="OO357_003741"
     CDS             2479..4014
                     /gene="lsrA"
                     /locus_tag="OO357_003741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter ATP-binding
                     protein LsrA"
                     /protein_id="HCR5888534.1"
                     /translation="MQTSDTRALPLLCARSVYKQYSGVNVLKGIDFTLHQGEVHALLG
                     GNGAGKSTLMKIIAGITPADSGTLEIGGNNYARLTPVHAHQLGIYLVPQEPLLFPSLS
                     IKENILFGLAKKQLSMQKMKNLLAALGCQFDLHSLAGSLDVADRQMVEILRGLMRDSR
                     ILILDEPTASLTPAETERLFSRLQELLATGVGIVFISHKLPEIRQIADRISVMRDGTI
                     ALSGKTSELSTDDIIQAITPAVREKSLSASQKLWLELPGNRPQHAVGTPVLTLENLTG
                     EGFRNVSLTLNAGEILGLAGLVGAGRTELAETLYGLRTLRGGRIMLNGKEINKLSTGE
                     RLLRGLVYLPEDRQSSGLNLDASLAWNVCALTHNLRGFWAKTAKDNATLERYRRALNI
                     KFNQPEQAARTLSGSNQQKILIAKCLEASPQALIVDEPTRGVDVSARNDIYQLLRSIA
                     AQNVAVLFISSDMEEIELMADRVYVMHQGEITHSALTGRDINVETIMRVAFGDSQRQE
                     ASC"
     gene            4008..5036
                     /gene="lsrC"
                     /locus_tag="OO357_003742"
     CDS             4008..5036
                     /gene="lsrC"
                     /locus_tag="OO357_003742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrC"
                     /protein_id="HCR5888535.1"
                     /translation="MLKFIQNNREITALLAVVLLFVLPGFLDRQYLSVQTLTMVYSSA
                     QILILLAMGATLVMLTRNIDVSVGSITGMCAVLLGMLLNAGYSLPVACVATLLLGLLA
                     GFFNGALVAWLKIPAIVATLGTLGLYRGIMLLWTGGKWIEGLPAELKQLSAPLLLGIS
                     AIGWLTIILVAFMAWLLAKTAFGRSFYATGDNLQGARQLGVRTEAIRIVAFSLNGCMA
                     ALAGIVFASQIGFILNQTGTGLEMKAIAACVLGGISLLGGSGAIIGAVLGAWFLTQID
                     SVLVLLRIPAWWNDFIAGLVLLAVLVFDGRLRCALERNLRRQKYARFMTPPPSVKPAS
                     SGKKREAA"
     gene            5036..6028
                     /gene="lsrD"
                     /locus_tag="OO357_003743"
     CDS             5036..6028
                     /gene="lsrD"
                     /locus_tag="OO357_003743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter permease LsrD"
                     /protein_id="HCR5888536.1"
                     /translation="MRIRYGWELALAALLVIEIVAFGAINPRMLDLNMLLFSTSDFIC
                     IGIVALPLTMVIVSGGIDISFGSTIGLCAIALGVLFQSGVPMPLAILLTLLLGALCGL
                     INAGLIIYTKVNPLVITLGTLYLFAGSALLLSGMAGATGYEGIGGFPMAFTDFANLDV
                     LGLPVPLIIFLICLLVFWLWLHKTHAGRNVFLIGQSPRVALYSAIPVNRTLCALYAMT
                     GLASAVAAVLLVSYFGSARSDLGASFLMPAITAVVLGGANIYGGSGSIIGTAIAVLLV
                     GYLQQGLQMAGVPNQVSSALSGALLIVVVVGRSVSLHRQQIKEWLARRANNPLP"
     gene            6040..7063
                     /gene="lsrB"
                     /locus_tag="OO357_003744"
                     /pseudo
     CDS             6040..7063
                     /gene="lsrB"
                     /locus_tag="OO357_003744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310150.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="autoinducer 2 ABC transporter substrate-binding
                     protein LsrB"
     gene            7090..>7183
                     /locus_tag="OO357_003745"
     CDS             7090..>7183
                     /locus_tag="OO357_003745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="involved in autoinducer 2 transport and processing;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase LsrF"
                     /protein_id="HCR5888537.1"
                     /translation="MADLDDIKDGKDFRTDQPQQNIPFTLKGCGA"
BASE COUNT         1676 a         1812 c         1876 g         1819 t
ORIGIN      
        1 tttcgtatgc agtgtctatg cgacagacac tgcgctctta gcatggggca gggaataacc
       61 aggctatttc tccatgcaca ggccaattat gaaagaggag aggcttaagt tcttgaaccc
      121 tgaacgaata acgactacca gaaaagcact tcgcatcact cagacatgat ttaacataat
      181 atacattatg cgtagtaata ttgtagtagc taaattgaga tgtccgtctc tgactaaata
      241 gacatgtccg cgctacagtc tcaccacagc gatcactgct ttcaaaggat gggactcatg
      301 acggcatacg gaacggagta atcggcaact gatgccgggc ctcgctgagc gtgcactgct
      361 tattatccgt gaacgtgccg ccaagaaccg cggtttgtgc cggacgcaca acgcctaacc
      421 caaggcaacc aagctgcacg tcgcctcctc caacgaccac cagagtgccc gccttcagac
      481 cgcagagttc cgccgcttgt gaacttacca cgcccagcaa tgtgccggtt tctttgacag
      541 gagaaagaat atcggcacgc aggccagcca tatccagcaa tgcaggtttc caggcacggg
      601 tggttagatc aagaagtccc gtggtgccag cgttagaggg atccaccgcc agttcgccgc
      661 tgagcatata ggccagccag tcgctgatca tggtgatggt tgatgcctga cggtaaatat
      721 cggaacgatg gtgcgccagc caaagtaatc tggggatggc acttaaagcc agtgtttgtc
      781 cggtcgctcg ataaacttcg ttttcaaagg tattgttgtg cagttcttta agttcgctaa
      841 cttcgcgtgc cgctctggca tccacattgg cgcaggccca gatcggtgtt ccttcattgt
      901 tatataaaac aatgccttca cgcatagaac atgccgaaac ggcagcgata tactccgggt
      961 ctatgccggc gttgtgcagc gcctggcgca tacactcaca cgccagttgc cagtttttgt
     1021 tgagatcaaa ttccatagaa ccaggaacgt ccggtactgc cagatgccgc cactccgcct
     1081 gtcccactgc tatttgattg ccttccaggt cgaatatcac agcccgaata cttccggtgc
     1141 ctgcatccag cgccatcagg tagtactttg attctgaagg ggtaaagagt cgagccatta
     1201 gattatcctc ggcttatgcg ttctgtctgt tcctctatac gttctccatc attcccggta
     1261 ataaggtcat gcaaatttaa ctacgtaaaa tcgccgctgc tgtgtcctga tcggtaacca
     1321 gtgcgttgat ataaccgcct ttcattgcag cggcaattgc ttcagcttta ttttctcccc
     1381 ctgccacgcc aacccggacg ggtatggtct tcagcgcgct taaaggtaag ccaatcagtt
     1441 cgttatgtat tttgatatcc gtgacaacgt caccttttgc atcaaaaaag tagcctaaaa
     1501 tgtcgccaac cgcccctttt cggccaatca ttaactgttc gccctggctg atataaccgg
     1561 agcgaatgat tgtcgcgtcg tcttgttgac tcacggctcc aatgccgaca atcgccacat
     1621 ccgctgcttg cgcggctagc agaacatctt tgacgcaatt ttcatttttt agcgtacggg
     1681 caatgtcagc ggaggatgcc cgcaacggag ccggaataat attcacactg cacgccgcgt
     1741 taagctgccc gatacccgtc atataagaac cgacgccacc ggagagcgtg accaggcgaa
     1801 tttgctgtga cgaaataaaa ccacttaagc gttgcagcgt attcatggtt gcctcgccaa
     1861 aaccaatcgc cagcatctgt tgtggttgaa gtaaactcat caacatatgc gccgcgccta
     1921 tccctagtcg cccgccgaca tcagcatccg caagcccagg gatcacccgg acatgttgca
     1981 gcgaaaactg acgacgtaat tgagtttcat attccagaca gccttcaaag cgagaattaa
     2041 tctgtacgcg aataatgccg gactgatgcc ctttctccag caagcgcgac actttcaaac
     2101 gggtcaggcc gagacgatcg ctgatctcgc tctgggtcag cccgtcgtga tagtaaaacc
     2161 acgcgatccg cgcgacctgt tcttcttcac acattccctg ttctgaaatt gccgaatcgt
     2221 tgattgtcat aattcattct tcactttgaa catatttaaa tctttaatgc aattgttcag
     2281 ttcttgcgca tttatatctg tgatggcaac cacagtttga ctctacgagc atgaacaaac
     2341 gcaaccgtga aaatcaaaat agcataaatt gtgatctatt cgtcggaaat atgtgcaatg
     2401 tccacctaag gttatgaaca aattaaaagt agaaatacat ttgttcaaaa ctcacctgca
     2461 aaactgaacg ggggaaatat gcaaacgagt gatacccgcg cgttaccgct actttgcgcc
     2521 cgctcggttt ataaacagta ttcaggggtc aatgtcctga aaggcatcga ttttacgttg
     2581 catcaggggg aggtccacgc cctgctcggc ggcaatggtg ccggtaaatc gacgttaatg
     2641 aagattattg ccggtattac ccctgctgat agcggtacgc tggagattgg gggcaacaac
     2701 tacgccagat taacgccagt tcatgctcat cagctgggca tttatctcgt tccccaggaa
     2761 ccgctgcttt tcccaagcct gtcgataaaa gaaaacatcc tgtttgggct ggcaaaaaaa
     2821 cagctctcca tgcagaaaat gaagaacttg ctggcggcgc tgggctgcca gtttgatctg
     2881 catagtctgg caggatcgct ggatgtcgcc gatcgccaaa tggtggaaat cctccgcggg
     2941 ctgatgcgcg actcgcggat tctgatcctc gatgaaccta ccgcctcgct tacccctgcg
     3001 gaaaccgaac gcttgtttag tcgcttgcaa gagctgcttg ctactggcgt gggtattgtt
     3061 tttatctcgc ataagctgcc ggaaattcgc cagattgccg atcgaattag cgtgatgcgc
     3121 gacggaacca tcgccttaag cggcaaaacc agcgaactgt ctaccgacga cattattcag
     3181 gccatcaccc cagcggtacg ggaaaaatcg ctctctgcca gccaaaaatt atggctggag
     3241 ttacctggta accgcccaca acatgccgtc ggaacgccgg tgctgacact ggaaaatctg
     3301 actggcgaag gcttcaggaa tgtcagcctg acgctcaatg ccggagaaat tctgggcctg
     3361 gctgggctgg tgggagccgg acgcacagaa ctggccgaga cgctctatgg tctgcgtact
     3421 ttgcgtggcg gacgcattat gctgaatggt aaagagatca ataaattatc cactggagaa
     3481 cgtttactgc gcggtctggt ttatctgccg gaagatcgcc agtcatccgg actgaatctc
     3541 gatgcttcgc tggcctggaa cgtctgcgcc cttactcata accttcgtgg attctgggcg
     3601 aaaaccgcga aagataatgc caccctggaa cgttatcgtc gggcgctgaa tattaaattt
     3661 aaccaaccgg aacaagctgc acggacatta tccggtagca accagcaaaa aatcctcatt
     3721 gccaaatgcc tggaagcttc gccgcaagca ttgattgtcg atgagccgac gcgcggcgtg
     3781 gatgtctcgg cccgtaatga tatctaccag ctgttgcgca gcatcgccgc acagaatgtg
     3841 gctgtgctgt ttatctcctc tgatatggaa gagatcgaac tgatggcaga tcgtgtgtat
     3901 gtgatgcatc agggcgaaat tacccactct gcactgaccg ggcgcgatat taatgtcgag
     3961 actattatgc gcgttgcctt cggcgatagt cagcgtcagg aggcatcatg ctgaagttta
     4021 ttcagaacaa ccgtgaaatc acggcattgc tggcggtggt gctgctgttt gtattacccg
     4081 gttttctcga ccgccagtat ttaagtgtgc aaacgctgac catggtttat agcagcgcgc
     4141 aaatcctgat cctgctggca atgggcgcga cgctggtaat gcttacgcgc aatattgatg
     4201 tttcagtggg ttcgattacc ggaatgtgcg cggtgctgtt ggggatgtta ctgaacgcag
     4261 gatattcact acctgttgcc tgtgtcgcga ctttactgct tggtttgctc gcgggatttt
     4321 tcaacggtgc cctggtcgcg tggctaaaga tccctgccat tgttgccacc cttggcacgt
     4381 tagggttgta cagaggcatc atgttgctgt ggactggcgg taaatggatt gaagggttac
     4441 ccgccgaact gaaacagctc tccgccccgc tgctgcttgg catttcagca attggttggt
     4501 tgacgataat tctggtggca tttatggcct ggctgctggc gaagacggcg tttggacgca
     4561 gtttttatgc cacgggcgat aatttacagg gcgctcgtca actgggcgtt cgtacggaag
     4621 ccattcgcat tgtggcattt tcgttgaacg gctgcatggc ggcactggcg ggaattgtgt
     4681 ttgcttcgca gattggtttt atcctcaacc agaccggtac cgggctggag atgaaagcaa
     4741 ttgcagcctg cgtgctgggc ggcattagtt tgctcggtgg ttccggtgcg atcatcggtg
     4801 cggtactcgg cgcatggttc ctgacgcaga tcgatagcgt actggtgctg ttgcgcattc
     4861 cggcatggtg gaatgatttt atcgcgggtc tggttctgct ggcggtgctg gtgtttgatg
     4921 gacgcctgcg ttgtgcgctg gaacgtaatc tacggcggca aaaatatgcc cgctttatga
     4981 cgccaccgcc atccgttaaa cccgcttcgt caggtaaaaa acgggaggcc gcataatgcg
     5041 tattcgctac ggttgggaac tggctcttgc cgcactgctc gttattgaga ttgtcgcatt
     5101 tggtgcaatt aacccgcgaa tgttagatct caatatgttg ctgttcagca ccagtgactt
     5161 tatctgcatt ggcattgtcg ctctaccgct gacgatggtg attgtcagtg gcgggatcga
     5221 tatttcgttt ggttcgacca tcggcctctg cgccattgca ttgggcgtac tgtttcaaag
     5281 tggtgtgccg atgccgctgg cgatactcct gaccttactg ctcggcgcat tgtgcgggct
     5341 gatcaacgcc ggattaatta tctataccaa agttaacccg ctggtgatta cgcttggcac
     5401 gctgtatctg tttgccggaa gcgctctgct gctttccggt atggccggag cgacggggta
     5461 cgaaggtatt ggtggattcc cgatggcgtt tacagatttc gctaacctgg atgtgctggg
     5521 actccccgtt ccgctgatta tcttcctgat atgtctcctc gttttctggc tctggctgca
     5581 taaaacccat gccggacgta atgtgttttt gattgggcaa agcccgcgcg tggcgcttta
     5641 tagcgcgatt ccagttaacc gcaccttatg tgcgctctat gccatgacgg ggctggcgtc
     5701 tgcggtcgcc gctgtgctgc tggtatcgta ttttggttca gcacgttccg atctcggtgc
     5761 gtcgtttctg atgcccgcca tcaccgccgt ggtgcttggc ggggccaata tttatggtgg
     5821 ttccggttcc attatcggca ccgccattgc ggttttatta gtgggatatt tgcagcaagg
     5881 tttgcaaatg gcaggagtgc caaatcaggt gtccagcgcc ctttccggtg cgctgcttat
     5941 cgtcgttgtc gtaggtcgtt ccgttagcct gcatcgccag caaattaaag agtggctggc
     6001 gcgtcgggcc aataacccat tgccataaag gatatcttca tgacacttca tcgctttaag
     6061 aaaatcgcct tacttagcgc tcttggcatt gccgcaatct ctatgaatgt gcaggccgca
     6121 gagcgtattg catttattcc caaactggtt ggcgtgggat tttttaccag cggtggcaac
     6181 ggcgcacaac aagcgggtaa agagctgagc gttgatgtga cctacgacgg gccgacagaa
     6241 cccagtgttt ctggtcaggt acagttgatt aataacttcg tcaatcaagg ttataacgcc
     6301 attattcgtt tctgcggttt cgcctgatgg cttgtgtccg gcactgaaac gcgccatgca
     6361 acgtggtgtg agagtgctga cctgggactc tgatactaaa ccggagtgcc gctcttacta
     6421 cattaatcag ggaacgcccg cccagttggg aggtatgttg gtggatatgg cggcgcgtca
     6481 ggtgaataaa gacaaagcca aagtcgcgtt tttctactca agccccaccg ttacggacca
     6541 aaaccagtgg gtgaaagaag cgaaagcgaa aatcgccaaa gagcatccag gctgggaaat
     6601 tgtcactacg cagtttggct ataacgatgc cactaaatcg ttacaaaccg cagaaggaat
     6661 attaaaagcg tatagcgatc tcgacgccat tatcgccccc gatgccaacg ccctgcccgc
     6721 tgccgcacaa gccgcagaaa acctgaaaaa tgacaaagta gcgattgtcg gattcagtac
     6781 gccaaatgtg atgcgcccgt atgtagagcg cggcacggtg aaagaatttg gactgtggga
     6841 tgtggttcag caaggcaaaa tttcagtgta tgtcgcggat gtattattga aaaaaggatc
     6901 aatgaaaacg ggcgacaagc tggatatcca gggcgtaggt caggttgaag tctcgccaaa
     6961 cagcgttcag ggctatgact acgaagcgga tggtaatggc atcgtactgt taccggagcg
     7021 cgtgatattc aacaaagaga atatcggcaa atacgatttc tgatgtgcat tacttaaccg
     7081 gagtaagtta tggcagattt agacgatatt aaagatggta aagattttcg taccgatcaa
     7141 ccgcaacaaa atatcccttt taccctgaaa ggttgcggtg cgc
//
LOCUS       DAJTRC010000155         7108 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.155, whole genome shotgun sequence.
ACCESSION   DAJTRC010000155 DAJTRC010000000
VERSION     DAJTRC010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7108
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.155"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..1274
                     /locus_tag="OO357_003746"
     CDS             <1..1274
                     /locus_tag="OO357_003746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5888538.1"
                     /translation="AIMTGRAPARFGVYSNTDAQDGIPLTETFLPELFQNHGYYTAAV
                     GKWHLSKISNVPVPEDKQTRDYHDNFTTFSAEEWQPQNRGFDYFMGFHAAGTAYYNSP
                     SLFKNRERVPAKGYISDQLTDEAIGVVDRAKTLDQPFMLYLAYNAPHLPNDNPAPDQY
                     QKQFNTGSQTADNYYASVYSVDQGVKRILEQLKKNGQYDNTIILFTSDNGAVIDGPLP
                     LNGAQKGYKSQTYPGGTHTPMFMWWKGKLQPGNYDKLISAMDFYPTALDAADISIPKD
                     LKLDGVPLLPWLQDKKQGEPHKNLTWITSYSHWFDEENIPFWDNYHKFVRHQSDDYPH
                     NPNTEDLSQFSYTVRNNDYSLVYTVENNQLGLYKLTDLQQKDNLAAANPQVVKEMQGV
                     VREFIDSSQPPLSEVNQEKFNNIKKALSEAK"
     gene            1326..2483
                     /locus_tag="OO357_003747"
     CDS             1326..2483
                     /locus_tag="OO357_003747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707596.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic sulfatase maturase"
                     /protein_id="HCR5888539.1"
                     /translation="MHVTAKPSSFQCNLKCDYCFYLEKESQFTHEKWMDDSTLKEFIK
                     QYIEASGNQVYFTWQGGEPTLAGLDFFRKVIHYQQRYAGQKRIFNTLQTNGILLNNEW
                     CAFLKEHEFLVGISIDGPQELHDRYRRSNSGNGTFAKVIAAIERLKSYQVEFNTLTVI
                     NNVNVHYPLEVYHFLKSIGSKHMQFIELLETGTPNIDFSGHSENTFRIIDFSVPPTAY
                     GKFMSTIFMQWVKNDVGEIFIRQFESFVSRFLGNGHTSCIFQESCKDNLVVESNGDIY
                     ECDHFVYPQYKIGNINKSELKTMNSVQLTAQKKRISAKCQQCAYKPICNGGCPKHRIT
                     KVNNETVSYFCEGYKILFSTMVPYMNAMVELAKNRVPLYHIMDVAKQMENN"
     gene            complement(2449..2571)
                     /locus_tag="OO357_003748"
     CDS             complement(2449..2571)
                     /locus_tag="OO357_003748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009518784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888540.1"
                     /translation="MTKHPTGIYVGCLVKVIRRRLRMELKESVINYSPFVLQHP"
     gene            2774..4459
                     /locus_tag="OO357_003749"
                     /pseudo
     CDS             2774..4459
                     /locus_tag="OO357_003749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416013.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
     gene            4497..6857
                     /locus_tag="OO357_003750"
     CDS             4497..6857
                     /locus_tag="OO357_003750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TonB-dependent receptor plug domain-containing
                     protein"
                     /protein_id="HCR5888541.1"
                     /translation="MKRVLIPGVILCGADVAQAVDDKNMYMYFFEEMTVYAPVPVPVN
                     GNTHYTSESIERLPTGNGNISDLLRTNPAVRMDSTQSTSLNQGDIRPEKISIHGASPY
                     QNAYLIDGISATNNLNPANESDASSATNISGMSQGYYLDVSLLDNVTLYDSFVPVEFG
                     RFNGGVIDAKIKRFNADDSKVKLGYRTTRLDWLTSHIDENNKSAFNQGSSGSTYFSPD
                     FKKNFYTLSFNQELADNFGVTAGLSRRQSDITRADYVSNDGIVAGRAQYKNVIDTALS
                     KFTWFASDRFTHDLTLKYTGSSRDYNTSTFPQSDREMGNKSYGLAWDMDTQLAWAKLR
                     TTVGWDHISDYTRHDHDIWYTELSCTYGDITGRCTRGGLGHISQAVDNYTFKTRLDWQ
                     KFAVGDVSHQPYFGAEYIYSDAWTERHNQSESYVINAAGKKTNHTIYHKGKGSLGIDN
                     YTLYMADHISWRNVSLMPGVRYDYDNYLSNHNISPRFMTEWDIFADQTSMITAGYNRY
                     YGGNILDMGLRDIRNSWTESVSGNKTLTRYQNLKTPYNDELAMGLQQKIDKNVIARAS
                     EAHDQISKSSRTDSATKTTITEYNNDGKTKTHSFNLSFELAEPLHIRQVDINPQIVFS
                     YIKSKGNLSLNNGYEESNTGDNQVVYNGNLVSYDSVPVADFNNPLKISLNMDFTHQPS
                     GLVWANTLAWQEARKARIILGKTNAQYISEYSDYKQYVDEKLDSSLTWDTRLSWTPQF
                     LKQQNLTISADILNVLDSKTAVDTTNTGVATYASGRTFWLDVSMKF"
     gene            6914..>7108
                     /locus_tag="OO357_003751"
     CDS             6914..>7108
                     /locus_tag="OO357_003751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888542.1"
                     /translation="MRNLCFLLTLVATLLLPGRLIAAALPQDEKLITGQLDNDLRYMI
                     YPHAHPKDQVNLWLQIHTGSL"
BASE COUNT         2079 a         1556 c         1511 g         1962 t
ORIGIN      
        1 ccgcaataat gaccggtcga gctcccgccc gctttggtgt ctattccaat accgatgctc
       61 aggatggtat tccgctaaca gaaactttct tgcctgaatt attccagaat catggttatt
      121 acactgcagc agtaggtaaa tggcacttgt caaaaatcag taatgtgccg gtaccggaag
      181 ataaacaaac gcgtgactat catgacaact tcaccacatt ttctgcggaa gaatggcaac
      241 ctcaaaaccg tggctttgat tactttatgg gattccacgc tgcaggaacg gcatattaca
      301 actccccttc actgttcaaa aatcgtgaac gtgtccccgc aaaaggttat atcagcgatc
      361 agttaaccga tgaggcaatt ggcgttgttg atcgtgccaa aacacttgac cagcctttta
      421 tgctttacct ggcttataat gctccgcacc taccaaatga taatcctgca ccggatcaat
      481 atcagaagca atttaatacc ggtagtcaaa cagcagataa ctactacgct tccgtttatt
      541 ctgttgatca gggtgtaaaa cgcattctcg aacaactgaa gaaaaacgga cagtatgaca
      601 atacaattat tctctttacc tccgataatg gtgcggttat cgatggtcct ctgccgctga
      661 acggggcgca aaaaggctat aagagtcaga cctatcctgg cggtactcac accccaatgt
      721 ttatgtggtg gaaaggaaaa cttcaacccg gtaattatga caagctgatt tccgcaatgg
      781 atttctaccc aacagctctt gatgcagccg atatcagcat tccaaaagac cttaagctgg
      841 atggtgttcc cttgctgccc tggttgcaag ataagaaaca aggcgagcca cataaaaatc
      901 tgacctggat aacctcttat tctcactggt ttgatgagga aaatattcca ttctgggata
      961 attaccacaa atttgttcgc catcagtcag acgattaccc gcataacccc aacactgagg
     1021 acttaagcca attctcttat acggtgagaa ataacgatta ttcgcttgtc tatacagtag
     1081 aaaacaatca gttaggtctc tacaaactga cggatctaca gcaaaaagat aaccttgccg
     1141 ccgccaatcc gcaggtcgtt aaagagatgc aaggcgtggt aagagagttt atcgacagca
     1201 gccagccacc gcttagcgag gtaaatcagg agaagtttaa caatatcaag aaagcactaa
     1261 gcgaagcgaa ataactaaac cttcatgcgg cggatttttc cgccgcctta ttgagcgaga
     1321 tagcgatgca cgttacagcc aagccctcca gttttcaatg taatctcaaa tgtgattact
     1381 gtttttacct tgaaaaagag tcgcagttta ctcatgaaaa atggatggat gacagcactc
     1441 tgaaagagtt catcaaacaa tatatcgaag cgtctggcaa tcaggtctat tttacctggc
     1501 aaggcggtga acccactctg gctggcctgg attttttccg taaagttatt cactatcaac
     1561 aacgctatgc aggccaaaaa cgtattttta atacattaca aacgaatggc attttattga
     1621 ataatgaatg gtgtgccttt ctcaaagaac atgaatttct ggttggtatc tcgatcgatg
     1681 gcccccagga gttacatgac cgttacagac gcagtaattc aggtaacggt acttttgcaa
     1741 aagtgatagc agccatcgag cgtctgaaat catatcaagt agagtttaat acgttaaccg
     1801 tcattaataa cgttaatgtc cattatcctc ttgaggttta tcatttttta aaatctatcg
     1861 gcagtaaaca tatgcaattt atcgaattgc tagaaaccgg gacgccgaat attgatttca
     1921 gtggtcatag tgagaacaca ttccgtatca ttgatttttc tgtgcctccc acggcttatg
     1981 gcaagtttat gtcaaccatt tttatgcaat gggttaaaaa cgatgtgggt gaaattttca
     2041 ttcgtcagtt tgaaagcttt gtcagccgtt ttttggggaa tgggcataca agttgtattt
     2101 tccaggagtc ctgcaaggat aatctggttg ttgaaagtaa tggagacatt tacgaatgcg
     2161 accattttgt ctatccacag tacaaaattg gaaacattaa taaatctgaa ctcaaaacga
     2221 tgaacagtgt acaactgaca gcgcaaaaaa aacggatttc agcgaaatgt cagcaatgtg
     2281 catataaacc tatctgcaat ggcggttgtc ctaagcatcg tattactaaa gtaaacaatg
     2341 agactgtttc ttatttttgc gaaggttata aaatcctttt ttcaaccatg gtaccttata
     2401 tgaacgccat ggttgagtta gctaagaaca gagtaccgct ttaccacatt atggatgttg
     2461 caaaacaaat ggagaataat taataacgct ctcttttaac tccattctta gcctccttct
     2521 tatgacctta acaaggcacc cgacataaat tcctgtcggg tgtttcgtca tcatcatctt
     2581 cccttgcgaa aatcctggct tacgtggtat gcgtcagggc attagcaata cccttgatta
     2641 tcaatagcat aaccataacc actgaaaatt aaaaatttct atatatttca tattgttaaa
     2701 tgatgcttaa ataaatttca tcagtaaatc attgccatag ataatcattg gatggcacaa
     2761 tcaattttcg ctaatgataa ccattcccat tacgttttgt atgttgatag caaagtatct
     2821 ctgtctgctg aagccctttt ggctgcgtaa gaataataaa acttcagtgt tgttaatcat
     2881 tattatcctc gcaatgatcc ttggcgtggt taaaattcag gtctggttaa acgactggaa
     2941 taatgatttt ttcaatgcgc taagccagaa agaaaccgat aagctttggc agctcgttct
     3001 ttggttccct gccctgctgg ggatctttgt attaatatcc gcaaacaaaa catggttaat
     3061 taaactactg actattcgct ggcgcgaatg gttaacagat tactacctta atcgctggtt
     3121 tgcagataaa aactactact tcacgcagat ctacggcgag cataaaaata cagataaccc
     3181 tgaccaacgt atcgctgaag acattctctt actgatcaac aaaacgctca gtctttcatt
     3241 tggcttcatc cagtcactta gcatgctgat cacctttacc gttattcttt ggcaaagcgc
     3301 gggtacgctc tcattcactg ttggtggaac agaatggaat atccaggggt atatggtcta
     3361 taccgtcgtg ctcattgtga tcggtggaac tttatttacc cataaggtgg gtaaacgtat
     3421 tcgtccgctt aatgtggaaa aacaacgtag cgaagcgaca tttcgaacta atcttgtgca
     3481 gcataataag caggcagaac tgattgcgct aagcaatgct gagtctctac aacgccagga
     3541 gttgcgcgac aattttcata ccattaaaga gaactggcat cgtttaatga atcgtcagcg
     3601 gtggcttgat tactggcaaa atatttattc gcgctcgctt agcgttcttc cctactttct
     3661 gttattgccg cagtttatta gcgggcagat caatctgggc ggactgatga aatcgcgcca
     3721 ggcatttatg ctagtatcga acaatttaag ctggtttatt tataaatatg acgaacttgc
     3781 tgaactggct gcggttatcg atcgcttgta tgagttccat caactcactg aatagcgccc
     3841 tacgaataag cctaaaaatt gccaacatgc ggtacaagtg gctaatgcga gtattcgtac
     3901 gcctgataat aagatcatat tagagaactt gaactttcat gtttcgccag gcaaatggct
     3961 attactgaaa ggctactctg gtgcgggaaa aaccacactg cttaaaacat tatcccactg
     4021 ctggccgtgg tttaaaggtg atatttcttc tcctgctgac agttggtatg tgtcacaaac
     4081 accgttaatc aaaagcggct tactgaaaga gattatttgt aaagcacttc ccctgcccgt
     4141 agacgataaa tcgttgagcg aagtactgca tcaggttggt ctgggaaaat tggctgcgcg
     4201 tattcatgac cacgatcgct ggggagatat tctttccagc ggcgaaaaac aacgtatcgc
     4261 cctggcacga ttaattttac gacgtccgaa atggatattt cttgacgaaa ctacctctca
     4321 tcttgaggaa caagaggcta tacgcttact gcgtttagtg cgtgaaaaac tacccacaag
     4381 cggcgtcatt atggttacac atcaacccgg tgtctggaac ctggctgatg atatttgtga
     4441 cattagcacg gttttataac aacaataaaa gccctgctgt acggagatat aaataaatga
     4501 agcgagttct tattcctggc gtcattttat gtggcgctga tgtggcgcag gccgtcgatg
     4561 acaaaaacat gtacatgtat ttttttgaag agatgacggt ctatgctcct gtccctgtac
     4621 ccgtaaacgg caacacgcat tacaccagtg aaagcatcga gcgtttaccg accgggaatg
     4681 gcaatatcag cgatctgctg agaaccaacc ctgcggtacg catggattca acgcaaagta
     4741 cctcgttgaa ccagggagat attcgccctg agaaaatctc tattcacggt gcgtcgccct
     4801 accagaatgc ctatttgatt gacggtatta gtgcaactaa taacctgaac ccagcgaatg
     4861 agtccgatgc cagtagtgca accaatatta gcgggatgtc acaggggtat tatcttgatg
     4921 tcagcttact ggacaatgtg acgctttatg acagttttgt gccggttgaa tttggtcgct
     4981 tcaatggcgg ggtaattgat gcaaagatca aacgcttcaa cgctgatgat agcaaggtga
     5041 aattgggtta tcgcactacg cgtttggact ggttaacatc gcatatcgat gagaataaca
     5101 agagcgcatt taatcaaggt tcttcaggaa gtacctattt ctctccagat tttaaaaaga
     5161 acttttatac cttgtcgttt aatcaggagc tggctgataa ctttggcgtt accgccggtt
     5221 tatcgcgccg ccagtctgat atcacccgcg cggattatgt ttcgaatgac ggcattgtcg
     5281 ccggtcgggc acagtataaa aacgttatcg atactgcatt gagcaaattt acctggtttg
     5341 ccagcgaccg ctttacccac gatttaacct taaaatatac cggctccagc cgtgattata
     5401 ataccagcac cttcccgcag tctgatcgcg aaatgggtaa taaatcctat ggtctggcat
     5461 gggatatgga tacgcaactc gcatgggcca aactacgtac caccgttggt tgggatcata
     5521 ttagtgatta tacccgtcac gatcatgaca tctggtacac cgaactttca tgtacatatg
     5581 gtgatattac agggcgttgt acccgtggcg gattaggaca catttcccag gctgtagata
     5641 attacacctt caaaacacgc ctggactggc aaaaattcgc cgtgggtgat gtttcgcatc
     5701 aaccctactt cggcgcggaa tacatctatt ccgatgcatg gactgaacgc cataaccagt
     5761 ctgaatccta tgtgattaat gctgcaggaa agaaaactaa ccataccatt taccataaag
     5821 gtaaaggcag cctgggaatt gacaactaca cgctgtatat ggcggatcac attagctggc
     5881 ggaatgtgtc gttaatgccc ggtgtgcgtt atgactatga caactatctg tcaaaccaca
     5941 atatctcccc gcgctttatg acggaatggg atatttttgc tgatcaaacc tcaatgatta
     6001 ccgccggtta taaccgttac tatggcggga atattcttga tatgggatta cgtgatatcc
     6061 gcaatagctg gacggaatcg gtatcaggta ataaaaccct gacgcgttat cagaatttga
     6121 aaacgcctta taacgatgaa ctggcaatgg gattgcagca aaaaatcgat aagaacgtta
     6181 ttgcacgcgc aagtgaagcg catgatcaaa tcagcaaaag cagtcgtacc gacagcgcga
     6241 ctaaaaccac cattactgaa tataacaacg atggcaaaac caaaacgcat tcgtttaacc
     6301 tcagttttga actggccgaa cccctgcata tccgccaggt agatattaac ccgcaaattg
     6361 tctttagcta tatcaagagc aagggcaact tgtcgttaaa caatggttat gaggagagca
     6421 ataccggtga taaccaggtg gtttataacg gtaatctggt ctcttacgat agcgttccag
     6481 tggcagattt taataaccca ttaaagatct ccttaaacat ggatttcacg catcaaccga
     6541 gcgggttagt gtgggcgaat acgctggcct ggcaagaagc gcgtaaagct cgcattatcc
     6601 ttggtaagac aaatgcgcaa tacatcagcg aatattcaga ttacaagcag tatgttgacg
     6661 aaaaactgga tagcagcctg acctgggaca cccgcttgtc ctggacgcca caatttctga
     6721 aacaacaaaa cctgacgatc agtgccgata ttctcaatgt actggatagc aaaaccgctg
     6781 ttgatacaac gaacaccggt gtggcgacct acgccagtgg ccgtactttc tggcttgatg
     6841 tcagcatgaa attttaatag ttcggatttt acttaaccgg gtaaattcgc ccggttttcg
     6901 catggagata attatgagaa acctctgttt cttactgacg ttagtggcaa ctctgttgct
     6961 ccccgggcgg ctgattgccg ccgccttacc gcaggatgaa aagttaatta ccgggcaact
     7021 ggacaatgac ttgcgatata tgatttatcc gcatgctcat ccaaaggatc aggtaaattt
     7081 atggctgcaa attcataccg gttcattg
//
LOCUS       DAJTRC010000156         7070 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.156, whole genome shotgun sequence.
ACCESSION   DAJTRC010000156 DAJTRC010000000
VERSION     DAJTRC010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 7070)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7070)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7070
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.156"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..302
                     /locus_tag="OO357_003752"
     CDS             <1..302
                     /locus_tag="OO357_003752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5888543.1"
                     /translation="SLLSSQLGDGKQFSCSRDFAASTGLVPRQYSTGGKSTLLGISKR
                     GDKNLRRLLVQCARSFMMQLERQHGKLAEWVREQLNKKHSNVVACALANKLARIA"
     gene            complement(641..1411)
                     /locus_tag="OO357_003753"
     CDS             complement(641..1411)
                     /locus_tag="OO357_003753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888544.1"
                     /translation="MNETLNALICRHARNLLLAQGWPEETDVDQCNPNYPGWISIYVR
                     LDAPRLATLLVNRHDGVLPPHLASAIQKLTGTGAELVLSGSQWQSLPVLPADGTQVSF
                     PYAGEWLTEDEIRAVLDAVRDAVCSVSCRGAEDARRIRAALTTSGQTLLTRQTRRFRL
                     VVKESDHPCWLDEDDENLPVVLDAILNRGARFSAVEMYLVSDCIEHILSSGLACDVLR
                     IPDEPPRRWFDRGVLREVVREARAEIRSMADALAKIRK"
     gene            complement(1456..1890)
                     /locus_tag="OO357_003754"
                     /pseudo
     CDS             complement(1456..1890)
                     /locus_tag="OO357_003754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001562898.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1380 domain-containing protein"
     gene            complement(1902..2123)
                     /locus_tag="OO357_003755"
     CDS             complement(1902..2123)
                     /locus_tag="OO357_003755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888545.1"
                     /translation="MNYAGHEKLRAEVAEVANAMCDLRTTMNEMERRYSFNADTLPER
                     LVRQTLFRANRLLMEAYTEILELDSCFKD"
     gene            complement(2124..2807)
                     /locus_tag="OO357_003756"
     CDS             complement(2124..2807)
                     /locus_tag="OO357_003756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858331.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methylase"
                     /protein_id="HCR5888546.1"
                     /translation="MSRFVLGNCIDVMARIPDNAIDFILTDPPYLVGFRDRQGRTIAG
                     DKTDEWLQPACNEMYRVLKKDALMVSFYGWNRVDRFMSAWKNAGFSVVGHLVFTKNYT
                     SKAAYVGYRHECAYILAKGRPRLPQNPLPDVLGWKYSGNRHHPTEKPVTSLQPLIESF
                     THPNAIVLDPFAGSGSTCVAALQSGRRYIGIELLEQYHRAGQQRLAAVQRAMQQGAAN
                     DDWFMPEAA"
     gene            complement(2871..3182)
                     /locus_tag="OO357_003757"
     CDS             complement(2871..3182)
                     /locus_tag="OO357_003757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858330.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888547.1"
                     /translation="MKYDGDGRATARFFSDKGCRRAPLFTAPADAARHKRCLWSVSRV
                     RRARDGRFYRSRLVSVTVYASPSPFSDERPSSRFRGITLLSKRRRLRYSTVGLTRYRK
                     R"
     gene            complement(3179..4081)
                     /locus_tag="OO357_003758"
     CDS             complement(3179..4081)
                     /locus_tag="OO357_003758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1281 domain-containing protein"
                     /protein_id="HCR5888548.1"
                     /translation="MYFPGEPAQIAEIKRLASGAVTPLYRRATNEGIQLFLAGSAGLL
                     QITENIRSEQCPGVTAAGRGAVSTENIAFTRWLTHLQNGVLLDEQNCLMLHELWLQSG
                     TGQRRWEGLPDDARETITVHFTAKRGDWCDIWGNEDVSVWWNRLCDNVVPEKTMPFDL
                     LTVLPTRLDVEVNGFNGGVLNGVPSAYHWYTERYGVKWPCGYDLNISSQGENFIQVDF
                     DTPWCQPESDVIAELSRRFSCTLEHWYAEQGCDFCGWQLYERGELVNVLWGELEWSSP
                     TDDDEQPEVTGPAWIVDNVAHYGG"
     gene            4521..5480
                     /locus_tag="OO357_003759"
     CDS             4521..5480
                     /locus_tag="OO357_003759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid segregation protein ParM"
                     /protein_id="HCR5888549.1"
                     /translation="MLKVSCDDGSTNVKLAWLEDGEVRTSLSGNSFKEGWNPGLFNAG
                     KVYNYVVDEKKYTYDLGSTAVIGTTHVSYQYSTTNLLAIHHALLTSGLQPQDVELTVT
                     LPVTEFFDNDNQPNEERIERKKANVLREISLNKGETFKIKKVNVMPESLPAAFESLKK
                     DKVNKLERSLIIDLGGTTLDCGLILGAFEGISEIRGYSEIGTSRITHTVMNALTKAST
                     PCNYFIADELIKNRHDNEYLQTLINDVAEIKNISHVIDREVKSLAESIRQEISTFSGM
                     NRIYLTGGGAELIYPHIKQYFPNLKVNKVDEPQFALVKAMVHA"
     gene            5480..5875
                     /locus_tag="OO357_003760"
     CDS             5480..5875
                     /locus_tag="OO357_003760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid partitioning/stability family protein"
                     /protein_id="HCR5888550.1"
                     /translation="MESSDPKKRKKVVAYLHPALYPQDNLTQQTIDSLPVQMRGDFYR
                     QSLICGAALYSVAPRLLTLISVFFSEKITAENLVKLIEQTTGYTSTSIDISVLKNIIE
                     ASSENKSESITSKDDFEEQTRRNLSMLKK"
     gene            6058..6438
                     /locus_tag="OO357_003761"
     CDS             6058..6438
                     /locus_tag="OO357_003761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858326.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HCR5888551.1"
                     /translation="MTMATINARIDDDIKNQADEVLKLMNISQTQAIAAFYQYITEQK
                     KLPFVITSIVKTPHDLLRESTDMLAEALAVISNLQVWTEQQDGIGKAKLMEYYRRLDA
                     LYCCAKEKIGLLSDNRDAELGCVP"
     gene            6484..7070
                     /locus_tag="OO357_003762"
                     /pseudo
     CDS             6484..7070
                     /locus_tag="OO357_003762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611198.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
BASE COUNT         1911 a         1836 c         1729 g         1594 t
ORIGIN      
        1 ccagcttact atcgtcgcaa cttggtgacg ggaaacagtt ttcatgcagc agagattttg
       61 cggcttccac agggctcgtt ccccggcagt atagcactgg aggaaaatcg actctgttgg
      121 gtatcagtaa gcgaggtgat aaaaatttac gccggttact ggttcagtgt gcccgctcat
      181 tcatgatgca gcttgaacgc cagcatggaa aacttgctga gtgggttcgg gagcaactca
      241 acaagaagca ttcgaatgtg gttgcctgcg ctctggcaaa taaactggcc cgaattgcct
      301 aggcaatcac tactcagcaa aaagaatatc aggcctgata tatagatgct gacaaaactc
      361 aattagctta ttgcaaacaa taagatacaa gttaccgtct ggttttgcga agattgatta
      421 ttgatgacat gaacggcaaa tcgacctgat taaaaaccta acaggggaaa atggctcact
      481 gaagccgagg ggatttttag gattatcagg tgcggaactc atcgaggcgc gggcatccct
      541 tgcccacaga gacgccggat agatttaagc aagcccacca ttaatcaaaa atcagtgttg
      601 caaaaacggg ggtgaccata gattttcccc gccatcgtca tcatttccgg atttttgcca
      661 gggcatccgc catgctgcgg atttcggccc gggcttcccg gacaacttcc cgcagaacac
      721 cacggtcaaa ccaccggcgg ggcggttcat ccggtatacg cagcacatcg caggccagcc
      781 cactggacag gatatgctca atacaatcgc tgaccagata catttccacc gccgaaaaac
      841 gtgcgccccg gttcaggatg gcatcgagca ccacgggcag gttttcatcg tcttcatcga
      901 gccagcaggg gtgatcgctt tccttcacga ccaggcgaaa gcggcgcgtc tggcgggtca
      961 gcaacgtctg accgcttgtg gtcagcgccg cccggatacg ccgcgcatct tctgcccccc
     1021 ggcaactgac actgcatact gcatcgcgca ccgcatcaag aactgccctg atttcgtctt
     1081 cggtcagcca ttcaccggca tacgggaaag acacctgcgt tccgtccgcc ggaagtaccg
     1141 gcagcgactg ccactgactg ccggacagaa caagttccgc tccggttccg gtcagtttct
     1201 gaatggcgga ggccaggtgc ggcggcagca cgccatcgtg gcggttaacg agtaacgtcg
     1261 ccagccgggg cgcatccagc cgcacgtaaa tgctgatcca gcccggatag ttcgggttac
     1321 attggtcaac atccgtctct tccggccagc cctgcgccag cagcaggtta cgggcgtgac
     1381 gacaaatcag agcattcagc gtttcattca taattcatga actccctgta tcgccgccca
     1441 caggcggcga taacatcagt cgttttcgtg tgtgttgttt ttcagcaggc tgcgggcctg
     1501 atcaaccatc gcctgtcggc gggtaacgta ttccgggagg gggaaaccgt gatcccgggc
     1561 agcccattca cgtttccaca gtgcctgttc agcgatctga gtcagggtct ccagcagttc
     1621 agcagggacg gtcacctggc ggttttcgcg cacgttgctg ataatctcca tcgccgcggc
     1681 ggaagaaata ccggactcaa tccgctagtc ttccgggatg tcctccaggt gcgccaggac
     1741 ggtcctgatt tcatgatcgg acagggaaat atccattccg tcagcgaggg cctgaatctc
     1801 ttccggggac cagataacca gcatcagcgg cgtgtctccc gaatactttg ctgccagcgc
     1861 ccggcatagc gtttcgcatg ttccgtacat actctctccc ctcaatcttt gaagcaggaa
     1921 tccagttcca gaatttcggt atatgcctcc atcaagaggc ggtttgcgcg aaacaacgtc
     1981 tgacgcacca gacgttccgg cagggtgtca gcattaaagc tgtaccgccg ctccatctca
     2041 ttcatggttg tacgcaggtc gcacatggca ttggccacct ccgccacttc ggcgcgaagt
     2101 ttttcgtgtc cggcatagtt catttacgca gcctccggca taaaccagtc atcattcgcg
     2161 gccccctgct gcatggcccg ctgtacggca gccagtcgtt gctgtccggc acggtgatac
     2221 tgctcaagca gctcgatacc gatataccgg cgtccggact ggagggcggc gacgcaggtt
     2281 gaaccgctgc ctgcaaacgg gtccagcact atcgcattcg ggtgtgtgaa gctctcaatc
     2341 agcggttgca ggctggtaac gggcttttcc gtcgggtgat ggcggttgcc cgaatatttc
     2401 cagcccaaca cgtccggcag cgggttttgt ggcagacgtg gacggccttt tgccaggatg
     2461 taggcgcatt cgtggcgata gcccacatat gcggccttcg atgtgtagtt tttggtgaag
     2521 accaggtgac caacaacgct gaatcccgca tttttccagg cagacataaa gcgatcgacg
     2581 cggttccagc catagaagct caccatcagc gcgtcttttt tgagtacgcg gtacatttca
     2641 ttacaggccg gttgcagcca ttcatcagtt ttatcaccag caatggtacg cccctgacgg
     2701 tcacgaaaac cgacgagata tggcggatcg gtgaggatga aatcaatggc gttatcagga
     2761 atacgggcca taacatcgat gcagttaccg aggacaaaac gggacataac agatccttct
     2821 cttacagagg gcacccctgt ggtgcccgtt ccccgctgcc tgtgggctgt tcatcgcttc
     2881 cggtaacggg tcaggccgac cgtggaatac cgcagccgtc gtctttttga cagcaaggtt
     2941 atgccgcgaa agcggcttga agggcgttca tcagaaaagg gcgacgggct ggcgtacact
     3001 gtcaccgaca ccagccggga gcggtaaaag cgcccgtccc gtgcccgccg gacgcgggac
     3061 actgaccaca ggcagcgttt atgccgcgcc gcgtcagccg gcgcagtaaa aagaggagca
     3121 cggcgacatc ctttgtcgga aaagaaacgg gcggttgccc gcccgtctcc gtcatacttc
     3181 atccgccata atgcgccaca ttgtcgacta tccacgcagg tccggtgact tccggctgct
     3241 catcgtcatc tgtcggggaa gaccattcaa gttcccccca cagcacattg acgagctctc
     3301 cacgctcata caactgccag ccacagaaat cacagccctg ttcggcatac cagtgctcca
     3361 gcgtgcagct gaaacggcgg cttaattctg caataacgtc gctttccggc tgacaccacg
     3421 gagtgtcgaa atccacctga atgaagtttt ctccctgact gctgatattc aggtcatatc
     3481 cacaaggcca cttcacgcca taccgttccg tataccagtg atatgcagaa ggaacaccgt
     3541 tcagaacacc accattaaag ccgttcactt caacatccag gcgggtcggc agaaccgtca
     3601 gcaggtcaaa cggcatggtt ttttccggca ctacgttgtc acacagacgg ttccaccaca
     3661 ccgatacatc ctcgttaccc catatgtcac accagtcacc tctttttgcg gtgaaatgca
     3721 cagtgatggt ttccctggca tcatccggta atccttccca acgacgctgg ccagtaccgc
     3781 tctgtaacca gagttcatgc agcatcaggc agttttgttc atccagcaac acaccgttct
     3841 gcaggtgtgt cagccagcgg gtgaacgcga tattttccgt cgatacagca ccacgtccgg
     3901 cagccgtcac tccggggcac tgttcagagc ggatattttc agtgatctgc agaagtccgg
     3961 cacttccggc cagaaacagc tgaatacctt catttgtggc gcggcgataa agcggtgtga
     4021 ctgcaccgct ggccagtcgt ttaatctcag cgatctgtgc cggctcacca ggaaaataca
     4081 tacgattcga gcaccagttt ggcatgggaa ttctcctctc acatgcggcc tccatcaggc
     4141 cgtgccgcac ctgtaaccgg gcgaccgcat acgaatgcag gtgcggcccc gaagccgcag
     4201 cgcgtggagg aggaatgacg aaacgtgacc gggtgaggag gccggcgaca gcgacacact
     4261 tgcatcggat gcagcccggt taacgtgccg gcacggcctg gttaaatagt atccgtctca
     4321 gttcgtggcg caaaatgaga aaccaataag aactcaaaat gaaccacaac aagaacataa
     4381 agagactcta aaagagcaac accgtttgac atagactcaa aatgagtcac aataagaaac
     4441 aataaaaaac tcaaaaagat gcaccgacat aaaggatgct caatttgaga taaacacaaa
     4501 ctcaaaacga ggcaaaaaga atgctaaaag tatcatgcga tgatggttca actaacgtaa
     4561 aactggcctg gcttgaagat ggcgaggtaa gaaccagtct gtcaggaaac agctttaaag
     4621 aaggctggaa cccgggactg tttaatgccg gcaaagttta caattacgtc gtggatgaaa
     4681 aaaaatacac ctatgatttg ggaagcactg ctgtcatagg tacaacacat gtcagttatc
     4741 aatacagcac caccaatctg cttgccatac atcatgcact gctgacatcc ggccttcagc
     4801 cacaggatgt agaactgaca gtaacattac ctgtgaccga attctttgat aatgacaatc
     4861 aaccaaatga agaaagaata gaaagaaaaa aggcaaatgt tctccgtgag atatctctga
     4921 ataaagggga aacatttaaa attaaaaaag ttaatgtaat gcctgaatct ttgccagctg
     4981 catttgaatc actaaaaaaa gataaagtaa acaaacttga acgcagcctg attatcgatc
     5041 ttggaggaac cactctggat tgtggtttaa ttctcggtgc atttgaaggc atttctgaaa
     5101 tccgtggcta ttcagaaatc gggacatccc gcatcactca taccgtcatg aatgcgctga
     5161 cgaaagcctc cacaccatgt aattatttta ttgctgatga gttaataaaa aacagacacg
     5221 ataatgaata tctccagaca ttaataaatg atgtagctga aataaaaaac atttctcatg
     5281 taatagacag agaagtgaaa tctctggcag aaagcatcag gcaggaaatt tcaacattct
     5341 caggaatgaa ccgaatctat ctcactggcg gaggagccga gcttatttac cctcacataa
     5401 agcaatattt cccgaatctg aaagtgaaca aggtggatga acctcagttt gcattggtga
     5461 aagcaatggt gcatgcgtaa tggaaagcag tgatccaaaa aaaagaaaga aagttgtggc
     5521 atacctgcac ccggcacttt atccgcagga caacctgaca caacaaacaa tagactctct
     5581 cccagtacaa atgcggggag atttctacag acaatccctt atttgcggag cagctcttta
     5641 ttctgttgcc ccccgactgt taacgctaat cagcgttttt ttcagtgaga aaatcactgc
     5701 agaaaatctg gtgaaactta tagaacaaac aacaggatac acatccacat caattgatat
     5761 aagtgtgtta aaaaacatca tagaggcatc ttcagaaaac aaatcagaga gcatcacttc
     5821 aaaagatgat ttcgaagaac aaaccagacg caatctttct atgttaaaaa aataataaaa
     5881 gaagagcagg ttcagtcaat tacctgctct tcttttatac atactaaaaa atcagatttt
     5941 tacgaatatt cagattatca taaaaaatgc aacagacagg aatgcaataa ttttgcatta
     6001 tctcgttgct tgtaatgcaa atatgcatta ttgtcagtgt gtgacaataa cagagagatg
     6061 acgatggcga ccattaacgc acgtattgat gatgatataa agaaccaagc agatgaagtg
     6121 ctgaagctga tgaatatcag tcaaactcag gctattgcag ctttttatca gtatattact
     6181 gaacaaaaga aactgccatt tgtgatcacc tcaattgtaa aaacgccgca tgatttatta
     6241 cgtgaaagta cagacatgtt agctgaagct cttgcagtga tttccaatct acaggtgtgg
     6301 actgaacagc aggatgggat tggaaaagca aagctaatgg agtattaccg ccgcctcgac
     6361 gcactgtact gttgtgcaaa agagaaaata ggtcttcttt cagataaccg ggatgctgaa
     6421 ctaggctgtg tcccttaatc atctgagtta tagtaactgt ctgtttacac aacatattga
     6481 actatggctc gctacgacct tcccgatgag gcatggacca tcatcaagcc cctgttacct
     6541 cctgaacccg ccacaccacg ggccggacgc ccatgggctg agcaccgtaa aatcatcaat
     6601 ggcatgttct gggtgttatg ttccagtgca ccatggcgcg atttacccga acgatatggg
     6661 gcatggaaaa ctgtttataa ccgctttaac cgatggtcaa agtcaggcgt gattaatatt
     6721 attttcaaca ggttgctttc gctacttgat gcaaacggct ttattgactg gtctgccacc
     6781 gcgctggatg gcagtaatat ccgggcgctg aaatgtgccg ccggtgctca aaaaaacatc
     6841 ccgatatcga cggagataat gggccgggtc gctctcgcgg cggttttggc accaaaatcc
     6901 atctggcaac agacggaagt ggcctcccgt taaatatcgt gctaagcccc ggacaagctc
     6961 atgaaagcca gttcgcgcaa cgacttctgg acgggattgg cgttcagcgt cagaacggca
     7021 gcatgaaacg ccgtggacat gcggtactgg ctgataaagc gtattcaggg
//
LOCUS       DAJTRC010000157         6987 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.157, whole genome shotgun sequence.
ACCESSION   DAJTRC010000157 DAJTRC010000000
VERSION     DAJTRC010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6987)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6987
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.157"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..221)
                     /locus_tag="OO357_003763"
     CDS             complement(<1..221)
                     /locus_tag="OO357_003763"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5888552.1"
                     /translation="MKNVTVSQSCGKWYISIQAESEVSTPVHPSASMVGLDAGVAKLA
                     SLSDGTVFEPVNSFQKNQKTLARLQRQLSR"
     gene            complement(354..659)
                     /locus_tag="OO357_003764"
                     /pseudo
     CDS             complement(354..659)
                     /locus_tag="OO357_003764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001324015.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            727..1342
                     /gene="tnpA"
                     /locus_tag="OO357_003765"
                     /pseudo
     CDS             727..1342
                     /gene="tnpA"
                     /locus_tag="OO357_003765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015695610.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
     gene            1670..2752
                     /locus_tag="OO357_003766"
     CDS             1670..2752
                     /locus_tag="OO357_003766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin OmpD"
                     /protein_id="HCR5888553.1"
                     /translation="MKKLTVAISAVAASVLMAMSAQAAEIYNKDSNKLDLYGKVNAKH
                     YFSSNDADDGDTTYVRLGFKGETQINDQLTGFGQWEYEFKGNRAESQGSSKDKTRLAF
                     AGLKFGDYGSIDYGRNYGVAYDIGAWTDVLPEFGGDTWTQTDVFMTGRTTGVATYRNN
                     DFFGLVDGLNFAAQYQGKNDRTDVTEANGDGFGFSTTYEYEGFGVGATYAKSDRTNDQ
                     VIYGNNSLNASGQNAEVWAAGLKYDANNIYLATTYSETQNMTVFGNNHIANKAQNFEV
                     VAQYQFDFGLRPSVAYLQSKGKDLGAWGDQDLIEYIDVGATYYFNKNMSTFVDYKINL
                     IDKSDFTKASGVATDDIVAVGLVYQF"
     gene            complement(3241..4039)
                     /locus_tag="OO357_003767"
                     /pseudo
     CDS             complement(3241..4039)
                     /locus_tag="OO357_003767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415078.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
     gene            complement(4049..4600)
                     /gene="ybcL"
                     /locus_tag="OO357_003768"
     CDS             complement(4049..4600)
                     /gene="ybcL"
                     /locus_tag="OO357_003768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Raf kinase inhibitor-like protein YbcL"
                     /protein_id="HCR5888554.1"
                     /translation="MKKLIVSSVLAFITFSAQAAAFQVTSNEIKTGEQLTTSHVFSGF
                     GCEGGNTSPSLTWSGAPEGTKSFAVTVYDPDAPTGSGWWHWTVANIPATVTYLPTDAG
                     RRDGTKLPTGAVQGRNDFGYAGFGGACPPKGDKPHHYQFKVWALKTDKIPVDSNSSGA
                     LVGYMLNANKIATAEITPVYEIK"
     gene            complement(4769..5101)
                     /gene="emrE"
                     /locus_tag="OO357_003769"
     CDS             complement(4769..5101)
                     /gene="emrE"
                     /locus_tag="OO357_003769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMR family multidrug efflux protein EmrE"
                     /protein_id="HCR5888555.1"
                     /translation="MNPYIYLGGAILAEVIGTTLMKFSEGFTRLWPSVGTIICYCASF
                     WLLAQTLAYIPTGIAYAIWSGVGIVLISLLSWGFFGQRLDLPAIIGMMLICAGVLVIN
                     LLSRSAPH"
     gene            complement(5446..5760)
                     /gene="fliE"
                     /locus_tag="OO357_003770"
     CDS             complement(5446..5760)
                     /gene="fliE"
                     /locus_tag="OO357_003770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707823.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-basal body complex protein FliE"
                     /protein_id="HCR5888556.1"
                     /translation="MSAIQGIEGVISQLQATAMSARAQESLPQPTISFAGQLHAALDR
                     ISDTQTVARTQAEKFTLGEPGVALNDVMTDMQKASVSMQMGIQVRNKLVAAYQEVMSM
                     QV"
     gene            5974..6987
                     /gene="fliF"
                     /locus_tag="OO357_003771"
                     /pseudo
     CDS             5974..6987
                     /gene="fliF"
                     /locus_tag="OO357_003771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416448.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar M-ring protein FliF"
BASE COUNT         1991 a         1605 c         1488 g         1903 t
ORIGIN      
        1 cggcttaact gtcgctgaag tctcgccagc gtcttctggt ttttctgaaa actgtttaca
       61 ggctcaaaga ctgtgccatc tgacagcgag gcaagtttag ccacgccagc atccagcccg
      121 accattgatg ctgaagggtg aaccggagtt gatacttcac tttctgcctg aatactgatg
      181 taccacttac cgcaggactg gctgacagtg acatttttca caacacccgt gacctgacgg
      241 ctgttccggt agcgcatcca gccaagtttt ggcagaaaaa tacggctgtt ttcctgagcg
      301 agcttaacgc cctgcggata gcggaatgca tcattctgtc accgcttttt gaatcagggg
      361 aacgccgagc gcttctggaa aaagtttttg caggcctgct cagggtcttt cagtgactgt
      421 tgcaatggct gtgacggagc atctttaatc cattgcgttt cagtggcatt tttccactca
      481 accaatcagg aagccatttt cgcgtaaggg atgtctttgt taccggcctt atgattctca
      541 ttctgacgtg ccagagtacg actgaaaacg aaacgacatg cgcctgcgaa gcgcctcatt
      601 tcacgcgcct gttgaccacc gggtcttaac cggaatttga atacctgtag tcgtttcaca
      661 gggcaaatta tactttggtc tgtgaaaaaa actgatatcc gacgcggaag gcactgtgtt
      721 ttcctgatgc atgctcaccg ggtctttgtt accaagtacc gacgccagat ttttgaccat
      781 gacgcgacag aaaaactacg cacttacttt tcaaatgtat gtgcttattt tgaagctgaa
      841 ctggttgaaa tggatggcga accagatcac gtccatctgt taattaacta tccccccaaa
      901 ctggcgatat ccagtctggt aaacaaacag cctcaaaggc gtatcgggta ggttactgcg
      961 acgggatcga ccagatattg ctgccaggta ttaccgcaaa gatgttttgt ggcgtcccgg
     1021 ctattttgcc agtagctgcg gaggtgcgcc aatatccgtc atccgccaat acattgaaca
     1081 acagcaaaca cctggtcaga tgaaaaaacg agccttatat ccccgccctg aaggacgggc
     1141 ttttacggtg cactggataa gcctggagat tatctacacc tgcatggaca tcattgtact
     1201 atgccccgta catgctccat cacatcctca acatcaaagt attagagcct tgcaaaccat
     1261 tatgcgtctc agtttgtctc atctatctta cgcaaaaaaa atagaactaa tatcaagcgg
     1321 cgctatattg cgtattaaat aaaaccacct caattacatt aataaatcta aataaatatc
     1381 atgcaaataa tttccatggt tatttttagg ctatttaaat taaaaaaata aattatgagt
     1441 caatcataaa taacaacctt taatataatt tgactaccaa atttatattc gttcacaaaa
     1501 tagtcatgca ttgtgtgcaa tagaaacagt tcagataaaa atagggatct acttcacaat
     1561 ttaaatgaga aactaaaact tacatcttga aataatctca ttgattggat gaatatttgt
     1621 agtgcagtga catcattttt aaataatagt tcaaataaaa gggctcacga tgaaaaaatt
     1681 aacagtggca atttctgctg tagctgcatc agtactgatg gcgatgtctg ctcaggcagc
     1741 agagatttac aataaagaca gtaacaagct ggatctgtac ggaaaagtta atgctaagca
     1801 ctacttctct tctaacgatg cagatgatgg cgacacaact tatgttcgtc tgggcttcaa
     1861 aggcgaaact caaatcaacg atcaactgac tggtttcggt cagtgggaat atgaattcaa
     1921 aggcaaccgt gctgaatctc aaggttcttc caaagataaa acccgtcttg ccttcgctgg
     1981 cctgaaattc ggtgactacg gctccatcga ttatggccgt aactacggtg tagcatacga
     2041 tatcggtgca tggaccgacg ttctgccaga attcggtggc gatacctgga cccaaacaga
     2101 tgtgttcatg actggtcgca ccacaggtgt tgcaacctat cgtaacaacg acttctttgg
     2161 tctggttgat ggtctgaact ttgctgctca gtatcagggt aaaaatgacc gcactgacgt
     2221 aactgaagct aacggtgatg gtttcggttt ctccactact tatgagtatg aaggattcgg
     2281 tgtaggtgca acctatgcta aatctgaccg cactaatgat caggttatct acggtaacaa
     2341 cagcctgaat gcatctggtc aaaatgctga agtatgggca gctggtctga aatatgatgc
     2401 gaacaacatc tatctggcta ccacctattc tgaaacccag aacatgactg tttttggtaa
     2461 taaccatatt gccaacaaag cacaaaactt cgaagtagtt gcacaatatc agttcgactt
     2521 cggtctgcgt ccgtccgttg cttacctgca atctaaagga aaagacttgg gtgcgtgggg
     2581 tgatcaggac ctgattgaat atattgatgt aggtgcaacc tattacttca acaaaaatat
     2641 gtccactttt gttgattaca aaatcaacct gattgataag agcgatttca cgaaagcatc
     2701 tggcgttgct accgatgata tcgttgctgt aggattggtt taccagttct aatctgatta
     2761 ctcaaagata tgttgtggga ggctttgcct ccccaacata taagtggcgc tctcaagcca
     2821 ctttccttag aagcactatc ttgcttctga ctatataaat taccctttat tttctgggac
     2881 gtttcaacgc cccattttta ataatactca gtgaacaatt aacgtatcaa ttagatttaa
     2941 taacaacgat atccgtacct aacctgatat ctaatgtcat aagcatccct ttaattatcc
     3001 cctcaacctc ttgtaaattt cccactcttg agctccacat agcccaaacg ccggatgcaa
     3061 aaaaaatcat acgtgcatct ctgaaattgt gatggaggaa gtgagtatca attatatgtg
     3121 cttataaacg ttcccgattg gcacagaagt caccggattg ttcaggctcc aataggttga
     3181 gtatgtcgta tttattttcc aaaataaaaa tcgatgctgt tgctccttaa taaaatactt
     3241 ttactctctg gacgcttttc ttagctctct tggtgttata ttaaaacgat tatggaacct
     3301 ttcagtaaaa cgagaaggac acttgtaacc attttccctg gcaatctcgc tgatgggttt
     3361 tactgtcgtt tgtatagcag gacaacgcat tatttaacct cacgtcgtcc aatatacttt
     3421 gaaaacttat tccctcgctt gccagacggc gatgcaatgt ggaaacagaa atgtagagat
     3481 atctcgctac cttgtttgct gtccattttg ttctaggttc ggatagcatt aggttataac
     3541 aacgacttat taatgattgt ttactatctg ataaaagtaa acaattaaca tgatgtgctc
     3601 ctaatgaaag tagaacgccc attgctaagt gttcctgaat ttgcgttgag aagcctcggg
     3661 aaatagatgt ttttagttgc tcccaacaat atattaactc ggggttctga ggtaagcaga
     3721 aacctgtcgt gttacgaatt tgatcagtta tcgcataaaa tttttgaaac ttttcaatta
     3781 gatcaatggg ataataaagc atttctgcaa gataaagacc tgcttctgga taattcacaa
     3841 tataaaattc atatccacag ggaaataata ttatctgatt attatcaaca gttagactat
     3901 gcgtctccca attgataact ttttttccct gacggatacg acataaagct ggcataagag
     3961 gcttaaccct gtgaatctca tgatgtttat gcatccgtat ttcttcaatc tttaggttag
     4021 acttatctct tgccagcata ctcttaccct attttatctc ataaactggt gttatctcag
     4081 cggttgcaat tttattggca ttaagcatat aaccaactaa cgctccgctg gaattagaat
     4141 ccacaggaat cttatcagtt tttagagccc atactttaaa ctggtaatga tggggtttat
     4201 ctcctttggg aggacatgct ccaccaaatc ctgcataacc aaaatcattt cggccttgaa
     4261 cagcaccagt cggaagtttt gtaccatcac gtcttcctgc atcagtgggc aaatatgtta
     4321 cagttgctgg aatattagca acagtccagt gccaccatcc actgcctgta ggagcatctg
     4381 gatcatacac agttacggca aagcttttgg tgccttcagg agcaccagac caggttaatg
     4441 agggcgatgt attaccacct tcacacccaa atccagaaaa tacatgagac gttgtaagtt
     4501 gctctcctgt ttttatttca ttactagtga cctgaaatgc tgcggcctgc gcagaaaatg
     4561 ttatgaatgc caatacactt gaaacgataa gttttttcat aaaaacctca ttgttgtgac
     4621 ctatcgttat tttatttgat gtgccttgat ctcattatgc ataaaggcgc aatgatcgcg
     4681 caaatgcaat cataattata aaggtgcccc gaacattagc cataatatgc acaaatcaga
     4741 ttcctgttat ctcaaggaga attatttttt aatgcggtgc acttcgtgac aacaagttaa
     4801 tgactaacac accggcacaa atcaacatca tgcctatgat agctggcagg tccagtcgtt
     4861 gaccaaaaaa tccccaggaa agcaaactaa tcagaacgat accgactcct gaccagatag
     4921 cataagcaat ccctgtaggt atataagcca gcgtctgagc taataaccag aatgatgcac
     4981 aatagcaaat aattgtgcca acagagggcc ataaccgtgt aaacccctct gaaaacttca
     5041 ttaaggttgt accaatgacc tctgcaagta ttgcgccacc aagataaata taagggttca
     5101 tagcatattc tttcctgttc atactggaga gaattgtact acagtttgaa cacaactcac
     5161 cgttttcatc ctggtgtatg tgtatccaag gggctctggc gtggcaagag ataaaggcag
     5221 gaacaataat gatcagaagg aaccccaggc taacgaatga gttcactctt cctgggagca
     5281 aagcgaagac aaaacatgtt atcgcgttta cggatcgtgg atggcagaaa acaaccagga
     5341 tcaggtatcc attttaaacc agaaattgag tgagtttgcc ccatccatgc cccacgctgt
     5401 gggatcggat ataattaaac aagcatagtc aacaggttac acaacctaca cctgcatgga
     5461 catcacttcc tgatacgccg ccaccagctt attacgcacc tgaatcccca tttgcataga
     5521 aactgaggct ttttgcatat cggtcatcac atcgtttagc gccacgccgg gttcaccgag
     5581 ggtgaatttt tctgcctgcg tgcgggcgac tgtttgtgta tcgcttatgc gatcgagcgc
     5641 ggcgtgcagc tgcccggcaa aactaatggt cggttgcggc agtgattcct gcgcacgcgc
     5701 actcatcgcc gtagcctgta actggctgat aaccccttca atcccctgta tcgctgacat
     5761 tttcgtctcc cggataattt ctggtaacaa agcctaccag taagtcaata agacaaaggc
     5821 gctaaatagc aacaaaaaaa ctggtttatt ggcggataga aaaaacgaaa gcacaaataa
     5881 tgggagcgtc aatttttcga gtgtgctgac ccgagagtga gtcttgttcc actttgccaa
     5941 taacgccgtc cataatcagc cacgaagtgc gcgatgaatg cgactgcagc ccagacaaaa
     6001 tctcttgagt ggcttaatcg cctgcgtgcg aatccgaaaa ttccattgat tgttaccggt
     6061 tccgcggcag tggcggtcat ggtcgcactg atcctgtggg cgaaagcccc cgactaccgc
     6121 acattattca gcaatctttc cgatcaggat ggtggcgcaa ttgtcagcca actgacgcaa
     6181 atgaatattc cttaccgctt cagcgaagcc agcggcgcta ttgaggttcc ggcagataaa
     6241 gttcacgaac tgcgcctggc gcaacaaggt ttgcccaaag gcggcgcggt cggtttcgaa
     6301 ctgctcgatc aggaaaagtt tggtatcagc cagttcagcg aacaggtgaa ttatcagcgg
     6361 gcgctggaag gcgagctttc tcgtaccatc gaaactatcg gcccggtaaa aggggcgcgc
     6421 gtacatctgg caatgccgaa accgtcttta ttcgtccgtg aacaaaaatc cccttctgca
     6481 tcggtgacgg taaatctgtt acccggccgc gcactcgatg aagggcaaat tagcgctatt
     6541 gtacatctgg tttccagcgc ggttgctggt ctgccgcctg gaaacgtcac gctggtggat
     6601 cagggcgggc atctgttaac ccagtccaat accagcgggc gcgatcttaa tgacgctcag
     6661 ttgaaatatg ccagcgatgt cgaaggccgt attcagcagc gtattgaagc gatcctgtca
     6721 cctattgttg gtaacggtaa tattcacgcc taggtcacgg cgcaactgga cttcgccagt
     6781 aaagaacaaa cggaagaaca gtatcgccct aacggtgatg aatctcatgc ggcgcttcgt
     6841 tcacgccagc ttaatgagag cgagcaaagc ggttccggtt atccgggcgg cgtaccgggg
     6901 gcgttgtcga atcaaccggc acctgcgaat aacgcgccaa tcagcacgcc tccgacaaat
     6961 caaaataacc gccagcagca ggcgagc
//
LOCUS       DAJTRC010000158         6963 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.158, whole genome shotgun sequence.
ACCESSION   DAJTRC010000158 DAJTRC010000000
VERSION     DAJTRC010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6963)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6963)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6963
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.158"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(365..946)
                     /locus_tag="OO357_003772"
     CDS             complement(365..946)
                     /locus_tag="OO357_003772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000291745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888557.1"
                     /translation="MYYPVTDYIALALIISFLFLTLFICLLCLKHERIKKETIRQKNA
                     HILEHGWNATEFSWFRYGQYNETGIYISIEKTIIITITVSGGCFKKEYSIVSHMLVTD
                     TITEATLYENGLYTRHIRLSRPVSDSKYPLPPGSQLIKNMTLRLRLQDQQEETSVTLF
                     QGKMSTDGNNYYIIKGKVSSVLLLLKMLQINHA"
     gene            complement(993..4850)
                     /gene="sigA"
                     /locus_tag="OO357_003773"
     CDS             complement(993..4850)
                     /gene="sigA"
                     /locus_tag="OO357_003773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SigA"
                     /protein_id="HCR5888558.1"
                     /translation="MNKIYSLKYSHITGGLVAVSELTRKVSVGTSRKKVILGIILSSI
                     YGSYGETAFAAMLDINNIWTRDYLDLAQNRGEFRPGATNVQLMMKDGKIFHFPELPVP
                     DFSAVSNKGATTSIGGAYSVTATHNGTQHHAITTQSWDQTAYKASNRVSSGDFSVHRL
                     NKFVVETTGVTESADFSLSPEDAMKRYGVNYNGKEQIIGFRAGAGTTSTILNGKQYLF
                     GQNYNPDLLSASLFNLDWKNKSYIYTNRTPFKNSPIFGDSGSGSYLYDKEQQKWVFHG
                     VTSTVGFISSTNIAWTNYSLFNNILVNNLKKNFTNTMQLDGKKQELSSIIKDKDLSVS
                     GGGVLTLKQDTDLGIGGLIFDKNQTYKVYGKDKSYKGAGIDIDNNTTVEWNVKGVAGD
                     NLHKIGSGTLDVKIAQGNNLKIGNGTVILSAEKAFNKIYMAGGKGTVKINAKDALSES
                     GNGEIYFTRNGGTLDLNGYDQSFQKIAATDAGTTVTNSNVKQSTLSLTNTDAYMYHGN
                     VSGNISINHIINTTQQHNNNANLIFDGSVDIKNDISVRNAQLTLQGHATEHAIFKEGN
                     NNCPIPFLCQKDYSAAIKDQESTVNKRYNTEYKSNNQIASFSQPDWESRKFNFRKLNL
                     ENATLSIGRDANVKGHIEAKNSQIVLGNKTAYIDMFSGRNITGEGFGFRQQLRSGDSA
                     GESSFNGSLSAQNSKITVGDKSTVTMTGALSLINTDLIINKGATVTAQGKMYVDKAIE
                     LAGTLTLTGTPTENNKYSPAIYMSDGYNMTEDGATLKAQNYAWVNGNIKSDKKASILF
                     GVDQYKEDNLDKTTHTPLATGLLGGFDTSYTGGIDAPAASASMYNTLWRVNGQSALQS
                     LKTRDSLLLFSNIENSGFHTVTVNTLDATNTAVIMRADLSQSVNQSDKLIVKNQLTGS
                     NNSLSVDIQKVGNNNSGLNVDLITAPKGSNKEIFKASTQAIGFSNISPVISTKEDQEH
                     TTWTLTGYKVAENTASSGAAKSYMSGNYKAFLTEVNNLNKRMGDLRDTNGEAGAWARI
                     MSGAGSASGGYSDNYTHVQIGVDKKHELDGLDLFTGLTMTYTDSHASSNAFSGKTKSV
                     GAGLYASAIFDSGAYIDLISKYVHHDNEYSATFAGLGTKDYSSHSLYVGAEAGYRYHV
                     TEDSWIEPQAELVYGAVSGKRFDWQDRGMSVTMKDKDFNPLIGRTGVDVGKSFSGKDW
                     KVTARAGLGYQFDLFANGETVLRDASGEKRIKGEKDGRILMNVGLNAEIRDNLRFGLE
                     FEKSAFGKYNVDNAINANFRYSF"
     gene            complement(5211..6215)
                     /locus_tag="OO357_003774"
                     /pseudo
     CDS             complement(5211..6215)
                     /locus_tag="OO357_003774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003557916.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraF"
     gene            6200..6577
                     /locus_tag="OO357_003775"
                     /pseudo
     CDS             6200..6577
                     /locus_tag="OO357_003775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004902302.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6616..>6963)
                     /locus_tag="OO357_003776"
     CDS             complement(6616..>6963)
                     /locus_tag="OO357_003776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000555401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HCR5888559.1"
                     /translation="LVPGEYSSGNSIRPRGITKVGNSELRRLLYEAAWSYRTPAKVGA
                     WLIYYRPDSVTQYSKDIAWKAQQRLCSRYRTLTAKGKKSQVAITAVARELTGFMWDIA
                     LAAQSSFSQQKQN"
BASE COUNT         1734 a         1568 c         1573 g         2088 t
ORIGIN      
        1 catcacctgc tattaatccc cgagtcagta cttcgcggcc tgacgcgatc accctactca
       61 gtacttcaga gcccgcctcc ggtacctgcc tggcgatcgc ccctgtgcaa caaaataaat
      121 caaccgttat caaaccacga tcgttcgtat gcgccgccga tgacgcgcgc ctgaaaaacg
      181 cgtcaatatg agttccccgg aagcttacga taatacgctt atgaacatta caacacgctc
      241 atgcggaacc tggatggggg aaggaacggt gaagctgtta gcgaatgacc ggcgtttata
      301 caaacgccgg tcatgatgaa tgatatttca gaatatacct cgggctaaaa taccgggaca
      361 gagatcaggc gtgattaatc tgcagcattt ttaataacag aagtactgag cttactttac
      421 ctttgatgat gtaatagtta tttccgtcgg tactcatttt tccctgaaat aatgtcacgg
      481 atgtttcttc ctgctgatcc tgcaggcgca gtcgtaaggt catattttta atgagctgag
      541 atccgggtgg caaggggtat ttgctgtcag atactgggcg actgagtctg atgtggcgcg
      601 tatacagccc gttttcgtac agggtggcct ctgttatggt gtcagtgacc agcatgtggg
      661 agacaatgct gtattctttt ttaaagcacc caccagaaac ggtgattgta atgataatgg
      721 tcttctcgat cgagatgtaa atacccgttt cgttatactg tccgtatcgg aaccatgaga
      781 actcagttgc attccagcca tgctccagaa tatgtgcatt tttttgcctg atagtttctt
      841 tttttattcg ctcatgttta aggcataaca ggcagataaa taatgtcaga aaaagaaagc
      901 taataatgag agcaagagcg atatagtctg tcacagggta atacatattc tctccggtag
      961 aagaagggcc gcaaacgcgg cccgggctgt tatcagaaag agtaacggaa gttggcgttg
     1021 atcgcgttat ccacgttgta tttaccaaat gccgatttct caaactcaag accgaagcga
     1081 agattatcgc gaatttcggc gttgagacca acattcatga gaatacgacc gtctttttca
     1141 cctttgatac gtttctcacc ggacgcatca cgcagtacgg tttcaccgtt ggcaaacagg
     1201 tcaaactggt agccaaggcc ggcgcgggct gtgactttcc agtccttacc ggagaaggat
     1261 ttacccacat caacaccggt acgcccaatc agcggattaa agtccttatc cttcatggtc
     1321 acgctcattc cgcgatcctg ccagtcgaac cgtttacctg atacggcccc ataaaccagt
     1381 tctgcctgcg gctcaatcca ggagtcttct gttacatgat agcggtagcc tgcttcagca
     1441 cccacataca aggaatgaga actgtagtct tttgttccga gtccagcaaa ggtcgccgag
     1501 tactcattat catggtgaac atacttacta atcaggtcga tataggcacc agagtcaaat
     1561 atagcggaag catacagacc tgccccgacg gacttcgtct tgccactgaa tgcattactg
     1621 ctggcatgac tgtcggtata cgtcatagtc agaccagtga aaaggtcaag tccatccagc
     1681 tcatgttttt tatccacacc aatctgcaca tgggtgtagt tgtcactgta tccaccagaa
     1741 gctgaacctg ctccgctcat gatgcgggcc catgcaccgg cctcgccatt ggtgtcacgc
     1801 agatccccca ttcgtttatt caggttgttg acttctgtca ggaaggcttt gtaattaccg
     1861 gacatatacg attttgctgc accggaagat gctgtatttt cagccacctt atatccggtc
     1921 agggtccacg tggtatgttc ctgatcctct ttcgtgctga tcacaggaga tatgttgctg
     1981 aaacctatgg cctgagtact ggctttaaat atctctttat tgcttccttt tggggctgtt
     2041 atcaggtcaa cgtttaatcc tgagttatta tttcccactt tctgtatatc gaccgacaga
     2101 ctgttattgc ttccggttaa ctgattttta acaatgagtt tatccgattg atttacagac
     2161 tggctcagat cagcccgcat aatcacagca gtattagtgg catccagtgt gtttactgtc
     2221 acagtatgga aacccgaatt ctctatgtta ctaaacaaca aaagactgtc gcgggttttt
     2281 aatgattgca gggctgactg tccgtttact ctccataagg tgttatacat gctggctgag
     2341 gctgcaggag catcaatacc tccggtataa gaagtatcaa agccacccag caaacctgta
     2401 gccagcggtg tgtgtgtggt tttgtccagg ttatcttctt tatactggtc aacaccaaac
     2461 agaatagatg cttttttgtc tgattttata ttaccattga cccaggcata attttgagcc
     2521 tttaacgtgg caccatcttc tgtcatatta tatccatctg acatatagat tgccgggctg
     2581 tatttattat tttctgtagg ggtgcctgtt aatgtcaggg ttccggccag ttcaatagct
     2641 ttatctacat acatttttcc ctgggcggta acagtagctc ctttgttgat aatcaggtct
     2701 gtattaatta aggataatgc accagtcata gtaacagttg atttatcacc aacagttatt
     2761 ttgctgtttt gagcactcag actgccgttg aaactacttt cgcctgctga atccccggag
     2821 cgaagctgtt gtctgaatcc aaaaccttcg ccagtaatgt ttcttcctga gaacatgtca
     2881 atgtatgcag ttttatttcc cagaacaatt tgagagtttt tagcctctat gtgtcctttt
     2941 acattagcat cccggcctat actcagggtt gcgttttcta aatttaattt ccggaaatta
     3001 aatttacgac tttcccagtc gggctgggaa aaagaggcta tctgattgtt ggacttatat
     3061 tccgtattgt aacgtttatt tacagtgctt tcctggtcct ttatggcagc agaatagtct
     3121 ttttgacata aaaaaggaat tggacagttg ttattgcctt ctttaaatat ggcatgttct
     3181 gtcgcatgtc cttgtaatgt taactgtgca ttccggacag agatatcgtt tttgatatcg
     3241 actgagccat caaagatcag attggcatta ttgttatgtt gctgggtagt attgataata
     3301 tgatttatgc ttatattacc tgatacattc ccatggtaca tatatgcatc agtattagta
     3361 agtgataatg ttgattgctt cacgtttgag ttcgttacgg ttgttcccgc atctgttgct
     3421 gcgattttct gaaatgactg gtcatagccg tttagatcca gtgttccgcc atttctggta
     3481 aaatagattt cgccattacc gctttcgctt aaagcgtctt tggcatttat ttttaccgta
     3541 cctttacctc cggccatgta aattttattg aaggcttttt cagcactaag gatgacagtc
     3601 ccattaccta ttttaaggtt atttccctgt gctattttta catccagagt accactacct
     3661 attttatgca gattatctcc ggcaacgccc ttaacattcc attcaacggt ggtattatta
     3721 tcaatatcta tcccggcacc tttataagac ttatcttttc cgtacacttt atatgtctgg
     3781 ttcttatcga atataagccc gccaatgcca agatcggtat cctgcttgag cgttaatacc
     3841 cctcctcctg agacagacag gtccttatct tttataatcg atgataactc ttgtttttta
     3901 ccatccagct gcatagtgtt tgtgaaattc ttttttaaat tgtttaccag aatattatta
     3961 aataacgagt agtttgtcca ggctatattg gtactactga taaaaccaac tgtactggta
     4021 acaccatgga aaacccattt ttgttgttct ttatcatata gataagaacc agaaccacta
     4081 tcgccaaaaa ttggtgagtt tttaaaaggg gttctgttgg tataaatgta actcttgttt
     4141 ttccagtcca gattaaaaag acttgcgctt aacaagtcgg gattatagtt ttgtccaaac
     4201 agatattgtt tgccgtttaa tatcgttgag gttgttccgg cacctgctct gaagccaatt
     4261 atttgttcct taccgttgta gtttacgcca tatcttttca tcgcatcttc gggagaaagt
     4321 gagaagtcgg cactctccgt aacccctgtt gtttccacga cgaatttatt cagacgatga
     4381 accgaaaagt cgccagatga tactctgtta cttgctttat atgctgtctg atcccatgac
     4441 tgtgttgtta ttgcatgatg ctgtgtaccg ttatgagtcg cagtaacact gtacgcacct
     4501 ccaattgatg ttgttgcacc tttgttggaa acagcagaaa aatcaggtac aggtagttct
     4561 ggaaaatgaa atatctttcc atctttcatc attaattgaa catttgttgc acccggtctg
     4621 aactctcctc tgttttgagc aaggtcaaga taatcgcggg tccatatatt atttatatcc
     4681 agcattgctg caaatgctgt ttcgccataa cttccatata ttgaggataa aataataccg
     4741 aggataactt tctttcttga tgtaccgaca ctaactttcc gggtcagttc agaaacagca
     4801 actaatccac ctgtaatatg actatatttc agtgaataaa ttttattcat acgtgaactc
     4861 cgatatttct gagttgtggt tatgggatag catttaatta caacggggaa ctatcagtca
     4921 tggtacgtaa ctgataaggg tataatgata atcactgtca gttatatttg tatgacagat
     4981 gtaacaaaat gaaaaaaaag ataagcacaa gtgattgatt ggttagatat tgatcacttg
     5041 gtgtttgtat tttgaatttt gcgatcaaaa tttgatcttt gttgtttccg gtcaatcctt
     5101 tacaggtttt ctgtattatc cgggggagtt aagtctgtcg atgtccgaca gacttttgat
     5161 gatggaaaca acatgcagcg ataatgtgca tgctgttttg caggtggaac tcagaaggtg
     5221 aactggaact gtgcgaccgc accgtaagtg ttgtccgtgc cgatcaggcc tgcaacatca
     5281 aggtgaacca catcaaacgg tgagatcccc accccggccg tgaatgcact gccgtcgtta
     5341 ccggccagat tctgacggta accggcgcgc agctgtgccc atttccaggc attaaattct
     5401 gtgccaatgg cggcaaactg acggttgctg tcggaggtga aaccgctggc cggggtcaga
     5461 tccacatcaa atgcagtggt gaacatcgcg ttgtgccagg agacaccggc cgtcacctgc
     5521 gggcggatcc tgaaggtttc cgtgataccg ttcacttctt ttgtctcaat actacgggag
     5581 atcaggttct gtgcgaccag tcccagcgtc cagttgtcat ccaggtccgt actggcaccg
     5641 atgtcggcgt tgatgccatt tttcgtgttg tggtagcggt cgcctttgaa ggcgctgctg
     5701 tcataatttc tgaccagcac gttgtagttg aacaggtcga cgcgctggaa tttggggggg
     5761 aagcccagag accatttctg cccggccgtt tccagttccg tagccagagc aatacccaca
     5821 tcagtgatca gtgccgtgcg ggcacaggcc tgtgatgtca ggcggctttt gtccacctcc
     5881 tgcccggtgc tgtttgccac ctcttccagg taagtgaggt cagcatcaga gactttaccg
     5941 tcgacactga cggtgccgtg tgatttaacc atcagtgcgg cagagaggcg atcaccggga
     6001 agggccgcca cagcagaaac gcccagctgt gcggcggcat gtgtgttacg gaagtcccgg
     6061 aggtgttctt tcaggcgtgc ggcagcctca ggaacaccgt ggttactttc gatggcgcgg
     6121 tcgaaggcct tccactcatc actgatccgg tcggcgttgt cggtgatatt atccggattg
     6181 gagagctgcg cgccgacgga cggaagcagg atgctgggct catgcgcgac gcaaaatcca
     6241 cgatgtatat atcagtacta aaagcgcgac ggcggaagaa gctctgaaac taatcggtga
     6301 actgtacgcc atagagcacg atatacgcgg gttaccggtg tctgaacgcc tggcggtcag
     6361 gcaaatgcag agtagaccgc tcctgacttc cctgtataag ctgatgcagg agaaagaaca
     6421 cacgttatcg aagaaatgtc gtctgggaga tgcgttccgg tatatcagga agcactgggc
     6481 tgcgttgtgc aacttctgtg atgatggcct ggctgaggta gataataatg cagcagaaag
     6541 agcgcttcgt gcagtctgtc tcgggaagaa aactttttcg aggatcactc attccgtgtc
     6601 tgactgcttc agggtttaat tttgcttctg ctgactgaat gatgattggg cagcaagtgc
     6661 aatatcccac ataaatccag tcaactcacg agccaccgcc gtaatggcta cttgtgattt
     6721 tttccctttt gctgtcagag ttcggtaacg agaacacaat cgttgttgag ctttccatgc
     6781 aatatctttg gaatattgtg ttacagagtc cggtcggtaa tatataagcc atgctccaac
     6841 ttttgcaggt gtacgataag accaggcggc ttcgtaaagc agacgtctca gttcgctgtt
     6901 tccaactttt gttattcctc tgggacgaat actgtttcca ctggaatatt cacctggcac
     6961 cag
//
LOCUS       DAJTRC010000159         6801 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.159, whole genome shotgun sequence.
ACCESSION   DAJTRC010000159 DAJTRC010000000
VERSION     DAJTRC010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6801)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.159"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            18..1076
                     /locus_tag="OO357_003777"
     CDS             18..1076
                     /locus_tag="OO357_003777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005119414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HCR5888560.1"
                     /translation="MSPEQRRAICEAFESANNTHGLNLTAHKYPGLRGTLQTASTDCD
                     TIVEAAALLPAFDQAVEGNRHQDDYGSGLGMAEEKFHYYLDLNDRYVYFYEPVNVEYF
                     AMNNWSFLQSGSIGIDRKDIEKVFTGRTVLSSIYQDQRTKQNVMSLLTPVYVAGQLKG
                     IVLLDINKNNLRNIFYTHDRPLLWRFLNVTLTDTDSGRDIIINQSEDNLFQYVSYVHD
                     LPGGIRVSLSIDILYFITSSRKSVLFWILTALILLNMVRMHFRLYQNVSRENISDAMT
                     GLYNRKILTPELEQRLQKLVQSGSSVMFIAIDMDKLKQINDTLGHQEGDLAITLHDAY
                     KASDERLYVNKQNKNSRS"
     gene            1119..1622
                     /gene="yeaK"
                     /locus_tag="OO357_003778"
     CDS             1119..1622
                     /gene="yeaK"
                     /locus_tag="OO357_003778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mischarged aminoacyl-tRNA deacylase"
                     /protein_id="HCR5888561.1"
                     /translation="MTEMAKGSVTHQQLIALLSQEGANFRVVTHEAVGKCEAVSEIRG
                     TALGQGAKALVCKVKGNGVNQHVLAILAADQQADLSQLASHIGGLRASLASPAEVDEL
                     TGCVFGAIPPFSFHPKLKLVADPLLFERFDEIAFNAGMLDKSVILKTADYLRIAQPEL
                     VNFRRTA"
     gene            complement(1623..1727)
                     /locus_tag="OO357_003779"
     CDS             complement(1623..1727)
                     /locus_tag="OO357_003779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416302.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888562.1"
                     /translation="MNDQMFVETLIITSSFFAIAVVLVLSVLLIERIG"
     gene            1897..2343
                     /locus_tag="OO357_003780"
     CDS             1897..2343
                     /locus_tag="OO357_003780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HCR5888563.1"
                     /translation="MFDVTLLILLGLAALGFISHNTTVAVSILVLIIVRVTPLSTFFP
                     WIEKQGLSIGIIILTIGVMAPIASGTLPPSTLIHSFLNWKSLVAIAVGVIVSWLGGRG
                     VTLMGSQPQLVAGLLVGTVLGVALFRGVPVGPLIAAGLVSLIVGKQ"
     gene            complement(2300..3118)
                     /gene="nimR"
                     /locus_tag="OO357_003781"
                     /pseudo
     CDS             complement(2300..3118)
                     /gene="nimR"
                     /locus_tag="OO357_003781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416304.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator NimR"
     gene            3215..4395
                     /gene="nimT"
                     /locus_tag="OO357_003782"
                     /pseudo
     CDS             3215..4395
                     /gene="nimT"
                     /locus_tag="OO357_003782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416305.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="2-nitroimidazole transporter"
     gene            4450..4797
                     /locus_tag="OO357_003783"
     CDS             4450..4797
                     /locus_tag="OO357_003783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HCR5888564.1"
                     /translation="MNIQCKRVYDPAEQSDGYRVLVDRLWPRGIKKTDLALDEWDKEI
                     TPSTELRKAFHGEVVDFATFREQYRAELAQHEQEGKRLADIAKKQPLTLLYAAKNTTQ
                     NHALVLADWLRSL"
     gene            complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="OO357_003784"
     CDS             complement(4819..5073)
                     /gene="yoaF"
                     /locus_tag="OO357_003784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing lipoprotein YoaF"
                     /protein_id="HCR5888565.1"
                     /translation="MKIISFVLPCLLVLAGCSTPSQPEAPKPPQIGMANPASVYCQQK
                     GGTLIPVQTAQGVSNNCKLPGGETIDEWALWRRDHPAGEK"
     gene            5256..6281
                     /gene="dgcP"
                     /locus_tag="OO357_003785"
     CDS             5256..6281
                     /gene="dgcP"
                     /locus_tag="OO357_003785"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707323.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase DgcP"
                     /protein_id="HCR5888566.1"
                     /translation="MSDQIIARVSQSLAKEQSLESLVRQLLEMLEMVTDMESTYLTKV
                     DVEARLQHIMFARNSQKMHIPENFTVLWDYSLCKRAIDENCFFSDEVPDRWGDCIAAR
                     NLGITTFLSTPIHLPDGSFYGTLCAASSEKRQWSERAEQVLQLFAGLIAQYIQKEALV
                     EQLHEANAALIAQSYTDSLTGLPNRRAIFENLTTLFSLARHLNHKIMIAFIDLDNFKL
                     INDRFGHNSGDLFLIQVGERLNTLQQNGEVIGRLGGDEFLVVSLNNENADISSLRERI
                     QQQIRGEYHLGDVDLYYPGASLGIVEVDPETTDADSALHAADIAMYQEKKHKQKTPFV
                     AHPALHS"
     gene            6314..6412
                     /locus_tag="OO357_003786"
     CDS             6314..6412
                     /locus_tag="OO357_003786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YoaK family small membrane protein"
                     /protein_id="HCR5888567.1"
                     /translation="MRIGIIFPVVIFITAVVFLAWFFIGCYAAPGA"
     gene            6415..6489
                     /gene="yoaJ"
                     /locus_tag="OO357_003787"
     CDS             6415..6489
                     /gene="yoaJ"
                     /locus_tag="OO357_003787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein YoaJ"
                     /protein_id="HCR5888568.1"
                     /translation="MKKTTIIMMGVAIIVVLGTELGWW"
     gene            complement(6650..6801)
                     /locus_tag="OO357_003788"
                     /pseudo
     CDS             complement(6650..6801)
                     /locus_tag="OO357_003788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001683974.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1636 a         1660 c         1668 g         1837 t
ORIGIN      
        1 ctgctgagca gtcggaagtg tcgccagaac agcggcgcgc catctgcgag gcttttgagt
       61 ctgccaataa tacccatggc ttaaacctga ctgcccataa atacccgggc ttacgcggca
      121 cactacaaac cgcatccact gactgcgaca caattgtgga agctgcagca ctattacccg
      181 cttttgatca ggcagtggaa ggcaaccgcc accaggatga ttacggttca ggtcttggga
      241 tggccgaaga gaaatttcac tattatctcg atctcaatga ccgctatgtc tatttttatg
      301 agccggttaa tgttgaatac tttgcgatga ataactggtc cttcctgcag tcaggaagta
      361 ttggcatcga tcgcaaagat attgaaaagg tatttaccgg gcgtaccgta ttgtcgagca
      421 tttaccagga tcagcgtact aaacagaacg tgatgagttt gctgacgccg gtatatgtcg
      481 cagggcagct aaaagggatt gtgctgctgg atattaacaa aaacaatttg cggaatatct
      541 tttacactca tgaccgccct ctcctctggc gttttctcaa tgtcacgcta accgataccg
      601 attcggggcg cgacattatc atcaaccaga gcgaagataa tctgttccag tatgtcagtt
      661 acgtccatga cttacctggc ggcattcgtg tctcgttatc cattgatatt ctttacttta
      721 tcacgtcttc gaggaaaagc gttctgttct ggattttgac ggcgttaatt ttgctgaata
      781 tggtccggat gcacttccgt ttataccaaa atgtgtcgcg agaaaatatt agtgatgcga
      841 tgactggact gtataatcgc aaaattttaa cccctgaact ggagcagcgg ttgcagaaac
      901 tggtgcaatc cggttcttcg gtgatgttta ttgcaattga catggacaag ttaaagcaaa
      961 taaatgacac cctcggtcat caggaggggg atttagcgat tacgttacat gatgcgtata
     1021 aagcttccga tgagcgttta tatgtcaata agcagaacaa aaacagccgt tcatgataac
     1081 cttctgtggt tgtttgcttg taatctcagg agcgtgaaat gactgaaatg gctaaaggaa
     1141 gcgtgacgca tcagcagtta atcgctttat tatcacaaga aggtgctaac ttccgcgtcg
     1201 ttacccatga agcggtaggt aaatgtgaag cggtgtcaga aattcgtggt accgctctag
     1261 gccagggtgc taaggcactg gtatgtaaag tcaaaggcaa tggcgtaaat caacatgttc
     1321 tggcgatcct cgcagccgat caacaggccg atctgagcca actcgccagc catataggcg
     1381 gattacgcgc ttcgttggcc agcccggcgg aagttgatga actcacaggc tgtgtcttcg
     1441 gcgcgatccc ccctttcagc ttccatccaa aactcaaact ggttgccgac cctctactct
     1501 tcgaacgatt tgatgaaatc gccttcaatg cagggatgct ggataaatcc gttattttga
     1561 aaaccgcgga ttatttgcgc attgcgcaac cagaactggt caatttccgc cgcactgcgt
     1621 agctagccta tccgttcaat aagcagaacg gacaaaacca gcacaaccgc aatagcaaaa
     1681 aacgatgatg tgataatcag tgtctcgacg aacatttgat cgttcatagc atgcccccgg
     1741 gagatagctg ttttcacgtt attgttagcg tgcacaaatg gcagtttgat gacagttcgc
     1801 ctgatttttt atgcaaaaaa cgtaaagatt ttctacttcc ttcctgcagc aagcataaag
     1861 taagcaggct tattattttt tggcaaggaa accacgatgt ttgatgtcac tttgctgatc
     1921 ctgctcgggt tagctgcgct gggctttatc agtcataaca ccactgtcgc cgtttcaatt
     1981 ctggtgttga tcattgtccg cgtcacaccg ttaagcacct ttttcccgtg gattgaaaaa
     2041 caggggctaa gtatcgggat aatcattctg acgattggcg ttatggcccc catcgccagc
     2101 gggacgctac cgccctcaac cttgattcac tcgtttctga actggaaatc gctggtggca
     2161 attgcagtag gcgttattgt ctcctggctg ggtgggcgcg gcgtgacgtt aatgggcagc
     2221 cagccgcaac tggttgctgg gctgctggtg ggcactgttt taggcgtagc gctgtttcgc
     2281 ggcgtaccgg ttggcccgct tattgccgcg ggtctggttt cgctgattgt ggggaaacag
     2341 tagttaatcc cgcaatatat cggcctggcg tttgccccaa gcctttctta aacatagtaa
     2401 taaaggccgt tgttgaatca taccccagcg tatgagcaac tttctgtaca gtatcgcctt
     2461 tcactaatcc ctgaagcgcc ataatcagct gtaactgctg acgccactgg cgaaaactca
     2521 accccgtctc tttcactatc aggcgggcaa gattgcgttc actcatcgca aaaaagccag
     2581 cccattgccc caatgccccc cactcgacgg gccctttcgc catcatctcc accatcgtgc
     2641 ggattttagg atgagaagaa acgggcaggt gcagctgttg ttgtggctgc tgagggagtt
     2701 catcaaacag gacctgaatt aatctgcggg tcatcggctc agcacgctga gtcgtcgtcc
     2761 tgtttgccag ggttaaaatc aattcccggc ataagggcga tattttcaat gtgcaacatg
     2821 gtcggcattg tcactgcgct gggttcgata aacagaaaac agagttcagc gttagctgtc
     2881 acttgattgc tatgcttcac cccgcccggt atccaaaccg catattgtgg cggcaccatc
     2941 cacaaagcat tttccaccgt acaggtaatt gcaccatgta atgccagtat cagctgtcct
     3001 ttgcggtgct gatgtacagg actggttaac tcatccgttc cggcgtgaat acaaaatgcg
     3061 accgcggctt catggtgtcg gtgggttcat agccattgag attcaacctg tgcatcattt
     3121 tgtccgaact tagcgataat ttgtcatttt agcttgattc aacataacaa taaaaacggt
     3181 aaggtacagc ctcgtttgta acaatgagaa gcatatgacc tgttcaactt cattaagcgg
     3241 caaaaacagg attgtcctta tcgctggcat tctgatgatt gccacaacat tacgcgtcac
     3301 ctttaccggc gcagcgccgt tactggatac gattcgttcc gcttactcgc tgacgacagc
     3361 gcaaaccggc ttattaacca ccctgccatt attggccttt gcgctaatct cacctctggc
     3421 tgccccggtg gcgcgacgtt ttggtatgga acgtagcctg tttgccgcgt tacttttgat
     3481 ctgtgcaggt atcgcaattc gttctctccc ttcgccttac ttattatttg gcggtacagc
     3541 ggtcattggc ggtgggattg cattaggcaa tgtcttactg ccaggattaa ttaaacgcga
     3601 tttccctcat tccgtcgcca gacttaccgg cgcatattcc ctgacaatgg gagctgcagc
     3661 ggcactggga tcggctatgg tcgtgccgct ggctttgaac ggttttggct ggcaaggcgc
     3721 gttgttcatg ctgatgtgtt ttcctctgct ggctcttttt ttatggctgc cacagtggcg
     3781 aagtcagcaa catgcaaatt tgagtacctc acgcgcctta catactcggg gtatctggcg
     3841 ttcgccactt gcctggcagg taacattgtt tcttgggatc aactcactgg tctattacgt
     3901 gattattggc tggcttccgg cgatcctcat cagtcacggc tatagcgaag cacaggcggg
     3961 ttcactgcat ggtttgctgc aactagccac agcagctccc ggtttgctga tcgcactttt
     4021 cttacatcat gtgaaagacc agcgtggtat tgcagcgttc gttgccttga tgtgcgcagt
     4081 gggcgcggtt gggctctgct ttatgccagc gcacgcgatc acctggactc tgcttttcgg
     4141 ttttggttcc ggcgcaacaa tgatactggg gttgacgttc attggtcttc ggcaagttct
     4201 gcgcatcagg cggcggcact ctcggggatg gcacaatccg tcgggtattt gctggcagcc
     4261 tgtgggccgc cgctaatggg taaaatacac gatgctaacg gtaactggtc cgtaccactg
     4321 atgggtgttg caatactttc actactgatg gcgattttcg gactttgcgc cgggagagac
     4381 aaagaaattc gctaatatcc ggtgctatag tgacgtgaca aatcttgcgt gaaagggaga
     4441 acaaacacga tgaatattca gtgcaaacgc gtttatgatc cggctgaaca gagcgatggt
     4501 tatcgcgtac tggtcgaccg cctctggccg cgcggtatca aaaaaaccga tttagccctt
     4561 gatgagtggg ataaagaaat cacgccgtca acggaactgc gcaaagcctt tcacggcgaa
     4621 gtcgtcgatt ttgcaacctt tcgcgagcaa tatcgcgcag aactggcgca acacgagcaa
     4681 gaaggaaagc ggctggcgga catcgccaaa aaacagccgc tgaccctgct ctacgcagca
     4741 aaaaacacca cgcagaacca tgcgctggtg ctggccgact ggctacgtag cttgtgattt
     4801 tagtacggca tccggcggtt atttttcacc agccggatgg tcccgtcgcc acaatgccca
     4861 ttcatcaatc gtttcaccgc ccggtaattt gcaattgttg ctgacccctt gcgctgtctg
     4921 cactggaatg agcgtcccgc ccttctgctg gcaatagacc gacgccggat ttgccatacc
     4981 aatctgcggc ggtttaggtg cttctggctg agaaggggtt gaacaaccag ccaggaccag
     5041 caagcaaggc agaacaaaac tgataatttt catttattga tctcacatat ttatccaagc
     5101 ttagagtatc gcggtatcgt tttgttttgc agcactattt ttattacatt cactcaaaac
     5161 atattacgtc ttgtttcatc tttgttgatg atgttttatc atgcctgcaa agattaaata
     5221 atcagcactt acccgccgta tcctggagtt gttccgtgtc agatcagatt atcgcccgcg
     5281 tctcgcaatc ccttgccaaa gaacagtcac tggaaagtct ggtccgacag cttctggaga
     5341 tgctggaaat ggtcactgat atggaatcaa cctacctgac caaagtggat gtcgaagcgc
     5401 gcctgcagca tattatgttt gcccgtaaca gccagaaaat gcacatcccg gagaatttta
     5461 ccgtcttgtg ggattactcg ttatgcaaac gcgccattga tgaaaactgc tttttcagcg
     5521 atgaagtccc tgaccgttgg ggcgactgta ttgcggcacg caatcttggc atcaccacat
     5581 ttctgagcac gccaattcac ttaccggatg gatcattcta tggcacgctt tgcgccgcca
     5641 gcagtgagaa gcgccagtgg agtgaacgcg cggaacaggt tttacagtta ttcgccggac
     5701 tgattgcaca atatattcaa aaagaggcac tggttgaaca gctgcacgaa gccaatgctg
     5761 cgctgattgc gcaatcgtat accgactcgt taaccgggct accgaatcgg cgggcgattt
     5821 ttgaaaatct gacgacgctg ttttccctcg cccggcatct taaccataag ataatgatcg
     5881 cgtttatcga tctggataac ttcaaattaa tcaatgatcg ttttggtcat aatagtggcg
     5941 atctgtttct cattcaggtt ggcgagcgcc ttaatacgct ccagcaaaat ggcgaagtta
     6001 ttggtcgtct cggtggtgat gagtttttgg ttgtttcact aaacaacgag aatgcggata
     6061 tttcgtcgct gcgagaacgt attcaacagc aaatacgtgg agaatatcac ttaggtgatg
     6121 ttgatttgta ttatcccggt gccagtcttg gcatagtaga agtcgatcct gaaacaaccg
     6181 atgcagacag tgccctgcat gctgccgata ttgcgatgta tcaggagaaa aaacacaaac
     6241 agaaaacacc ttttgtcgcg catccagcgc tacattcctg aggcgtattc acatcctttt
     6301 gattggtgat aacatgcgaa tcggtattat ttttccggtt gtaatcttca ttacagcggt
     6361 cgtattttta gcatggtttt ttattggctg ctatgctgcc ccgggagcat aaagatgaaa
     6421 aaaacaacga ttattatgat gggtgtggcg attattgtcg tactcggcac tgagctggga
     6481 tggtggtaac gtcacctcta aaaaatagca aaggctgcct gtgtgcagcc tttgtgcaat
     6541 ttaagcgtta acttttaatc ttcctgtaga taaatagcac gacaatcgca ccaataacgg
     6601 ctgaagcggc gcacgaaaaa cgctttcgcg tttttcgtgc gccgcttcac ccctggttca
     6661 gcatttcgtc tgcagtctga accgcaggca accatttttc agcaagtctg cgctgggaat
     6721 cgctggcttt atcccatttg ctccacagtg cctcacgatc gtaatcgtgc gcttccagcg
     6781 tggggcgggc gatttcaaaa t
//
LOCUS       DAJTRC010000160         6795 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.160, whole genome shotgun sequence.
ACCESSION   DAJTRC010000160 DAJTRC010000000
VERSION     DAJTRC010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6795
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.160"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            240..2672
                     /locus_tag="OO357_003789"
     CDS             240..2672
                     /locus_tag="OO357_003789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HCR5888569.1"
                     /translation="MAELNLSNLTEADIITKCVMPAILNAGWDNTTQIRQEVKLRDGK
                     VIVRGKVAARRTVKSADIVLYHKPGIPLAVIEAKANKHEIGKGMQQGIEYARLLDVPF
                     VFATNGDGFIFRDATAAEGECLEKQITLDDFPSPAELWQKFCLWKGYTQAQLPVITQD
                     YYDDGSGKSPRYYQLQAINKTIEAVSNGQNRVLLVMATGTGKTYTAFQIIWRLWKSKN
                     KKRILFLADRNILVDQTKNNDFQPFGTAMTKVSGRTIDPAYEIHLALYQAITGPEEDQ
                     KAFKQVAPDFFDLIVIDECHRGSASEDSAWREILDYFSSATQIGLTATPKETHEVSST
                     DYFGDPVYVYSLKEGIEDGFLAPYKVVRVDIDVDLQGWRPTKGQTDLNGEVIDDRIYN
                     QKDFDRTMVIDERTELVARTITDYLKRTNPMDKTIVFCNDIDHAERMRRALVNLNPQQ
                     VKKNDKYVMKITGDDEIGKAQLDNFINPKKPYPVIATTSELMTTGVDAKTCKLVVLDQ
                     NIQSMTKFKQIIGRGTRIDERYGKLWFTILDFKKATELFADERFDGIPEKVMDTTPED
                     IADPESDFEEKLEEISEHDDEQVTGVDEPPAPPYQVTDTDDVGPFPEEDEKKIRKFHV
                     NGVAVGVIAQRVQYYDADGKLVTESFKDYTRKTLLKEYASLDDFTRKWQDADRKEAII
                     HELEQQGIIWEVLAEEVGKDLDPFDMLCHVVYGQPPLTRKERAENVHKRNYFTKYSEA
                     AQAVLDNLLDKYADAGVQEIESIQVLKLKPFDSMGTLPEIIKTGFGDRNGYNQALSEL
                     ENEIYQLPPRSA"
     gene            2739..4208
                     /locus_tag="OO357_003790"
     CDS             2739..4208
                     /locus_tag="OO357_003790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HCR5888570.1"
                     /translation="MSISSVIKSLQDIMRKDAGVDGDAQRLGQLSWLLFLKIFDAQEE
                     ALELEQDNYQYPIPQRYLWRSWAANAQGITGDSLLEFVNDDLFPALKNLTAPIDKNPR
                     GYVVKQAFSDAYNYMKNGTLLRQVINKLNEIDFTSASERHLFGDIYEQILKDLQSAGN
                     AGEFYTPRAVTRFMVDRVDPKLGESIMDPACGTGGFLACAFDHVKNKYVKSVADHQTL
                     QQQIHGVEKKQLPHLLATTNMLLHGIEVPVQIRHDNTLNKPLSSWDEQLDVIVTNPPF
                     GGTEEDGIEKNFPAEMQTRETADLFLQLIVEVLAKNGRAAVVLPDGTLFGEGVKTKIK
                     KLLTEECNLHTIVRLPNGVFNPYTGIKTNLLFFTKGQPTKEIWFYEHPYPAGVKNYSK
                     TKPMKFEEFQAEIDWWGNEADGFASRVENEQAWKVSIDDVIARNFNLDIKNPHQAETV
                     SHDPDELLAQYAKQQAEIQTLRNQLRDILGAALSGKEVN"
     gene            4208..5869
                     /locus_tag="OO357_003791"
     CDS             4208..5869
                     /locus_tag="OO357_003791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HCR5888571.1"
                     /translation="MSVEKLIVDHMETWTSALQTRSTAGRGSSGKIDLYGIKKLRELI
                     LELAVRGKLVPQDPNDEPASDLLKRIAAEKAELVKQGKIKKQKPLPEISEEEKPFELP
                     EGWEWVHLPDIYCSISESSRKIKSSEILPEGKYPVIEQSQEFISGYCNNECLLIKLNN
                     PVIVFGDHTRNIKFIDFDFVVGADGVKILSPILICERFFFWQLRSFKLDVRGYARHFK
                     VLNSCLFALPPIAEQERIVEKVSSLMSLCDQLEQQSLTSLDAHQQLVETLLGTLTDSQ
                     NTAELAENWARISEHFDTLFTTEASVDALKQTILQLAVMGKLVPQDPNDEPASELLKR
                     IAQEKAQLVKEGKIKKQKPLPPISDEEKPFELPEGWEWCRIGNIVNIKSELVSPKDYL
                     NLYQVAPDIIEKGTGRVISKRTVKESGVKGPNSRFYKGQIVYSKIRPSLSKVFLAEYN
                     GLCSADMYPLDCYINPNYLLKYILSIPFLMQVKKAENRIKMPKLNSDSFYNIIVAIPP
                     YNEQQAIFDKINSIEAVCNGLISYIGIYHKTQLHLADALTDAAIN"
     gene            6090..6431
                     /gene="symE"
                     /locus_tag="OO357_003792"
     CDS             6090..6431
                     /gene="symE"
                     /locus_tag="OO357_003792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endoribonuclease SymE"
                     /protein_id="HCR5888572.1"
                     /translation="MTDTHSIAQPLEAEVSPANNRQLTVSYASRYPDYSRIPAITLKG
                     QWLEAAGFATGTAVDVKVMEGCIVLTAQPAAAEESELMQSLRKVCKLSARKQRQVQEF
                     IGVITGKQKVA"
     gene            complement(6478..>6795)
                     /locus_tag="OO357_003793"
     CDS             complement(6478..>6795)
                     /locus_tag="OO357_003793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888573.1"
                     /translation="LFDHLRDEVMRLDAGITQEVLKLYIAFKAETNFVDVVPQKSRLR
                     LSLNMQFHELVDPKGIAKDVTNVGRWGNGDVEIGFSDLAQLPYIMGLIRQAFEKQMES
                     ALV"
BASE COUNT         2003 a         1612 c         1554 g         1626 t
ORIGIN      
        1 tttcttgttc gaaagaaacg caactataag atttttatat attttgttag agttgatatg
       61 gttcctgtaa cttctgttta tctgtctctg atatgcccag attaattcaa gcaagatata
      121 acagagatat caatgtgatc tctgctacgt actttacgtt gaaacaccgc cctaaatcat
      181 agccaggcta ttgattttat gtacaatcag aatcgtcctg ataaaagcga gaacccccca
      241 tggcggaact gaacctaagt aacctgacgg aagcagacat cattaccaaa tgcgttatgc
      301 cagccattct caatgcaggc tgggacaaca caacgcaaat cagacaggag gtcaaactcc
      361 gggacggtaa agtcatcgta cgtggtaaag ttgcggcacg cagaacggta aaatctgctg
      421 acatcgtgct gtaccacaag ccaggcattc ccttagccgt gattgaagca aaagccaaca
      481 aacatgaaat tggcaaaggg atgcaacagg gcattgaata tgcgcgcctg ctggacgttc
      541 cttttgtttt tgccaccaac ggtgatggct ttatcttccg cgatgccacc gcagccgaag
      601 gtgaatgcct tgaaaagcaa ataacgctgg atgacttccc ctcccccgct gaactctggc
      661 aaaaattctg cctctggaaa ggatacacac aagctcagct tccggtgatt actcaggact
      721 attacgacga tggcagcggc aaatcgcctc gttattatca acttcaggca atcaacaaaa
      781 ccattgaagc cgtctccaac gggcaaaacc gcgttctgct ggtcatggcg accggaacag
      841 ggaaaaccta taccgcattc cagatcatct ggcgcctgtg gaaatcaaaa aataaaaaac
      901 gcattttgtt ccttgccgat cgcaacattc tggtcgacca aaccaaaaat aatgatttcc
      961 agccatttgg tacggcaatg accaaagtca gcggacgcac cattgatccc gcttatgaaa
     1021 ttcacctcgc gctctatcag gctataactg gcccggagga agaccaaaaa gcgtttaaac
     1081 aagtcgcacc agatttcttc gatctgatcg tgatcgacga atgccatcgc ggcagcgcat
     1141 ccgaagacag cgcctggcga gagatccttg attatttcag ttccgccacc caaattggct
     1201 taaccgccac gccaaaagag acgcatgaag tctccagcac ggattacttc ggcgatccgg
     1261 tttacgtcta ctcactaaaa gaagggatcg aagacggctt cctcgctcct tataaagttg
     1321 tccgtgttga tattgatgtc gacctgcaag gctggcgccc aaccaaaggg caaactgact
     1381 taaacggcga agtgattgac gatcgtatct ataaccagaa agatttcgat cgcacgatgg
     1441 tgatcgacga acgcacagaa ctggttgcca gaaccattac cgactacctc aagcgcacca
     1501 atccgatgga taaaaccatc gtcttctgta acgacatcga tcatgcagaa cgtatgcgcc
     1561 gcgccctggt taatctcaac ccgcagcagg tgaaaaagaa cgacaaatac gtcatgaaaa
     1621 tcaccggcga tgatgaaatt ggcaaagctc agttggataa cttcatcaac ccgaaaaaac
     1681 cgtacccggt tatcgcgacc acttcagagc tgatgaccac tggtgtggat gctaaaacct
     1741 gcaaactggt agtactagac cagaacatcc agtcgatgac caaattcaag caaattatcg
     1801 gtcgtggtac acgcatcgac gaacgttacg gcaaactctg gtttaccatc ctcgacttta
     1861 aaaaagccac cgaactgttt gccgatgagc gtttcgatgg cattcccgaa aaagtcatgg
     1921 ataccacacc agaggatatc gccgatccag aatctgattt tgaagagaaa ctcgaagaaa
     1981 tcagcgaaca tgacgacgaa caggtaacag gcgttgatga accgcctgcg ccaccatacc
     2041 aggttaccga taccgatgat gtcggcccat ttccggaaga agacgagaag aaaatccgca
     2101 agtttcacgt caacggtgta gcggtgggcg ttattgcaca gcgcgttcag tattacgacg
     2161 ccgacggtaa actggttacc gaatccttta aagattacac ccgcaaaaca ctgctcaaag
     2221 aatatgcctc gctggatgac tttacccgca agtggcagga cgccgatcgc aaagaagcga
     2281 tcattcacga gctggagcaa caggggatca tctgggaagt actggcagaa gaagtcggta
     2341 aagatctcga cccgttcgac atgctttgcc acgtagtgta tggtcagccg ccgttaaccc
     2401 gcaaagagcg cgccgagaac gtgcacaagc ggaactactt cacaaaatac tctgaagcag
     2461 cgcaagccgt gctcgataat ctgctggata aatacgccga tgcgggcgta caggagatcg
     2521 aaagtattca ggtgctgaaa cttaaaccat tcgacagcat gggcacctta ccggagatta
     2581 ttaaaaccgg atttggcgac cgtaacgggt ataatcaggc gctcagcgag ctggaaaacg
     2641 aaatctacca attaccgccc cgctctgctt aacaactctc ttttgcgcct tctgcaacca
     2701 tcatgttgca gaaggccttg tttttcatgg aatacaacat gtctatcagc tcagtaatca
     2761 aatcattaca agatattatg cgcaaagatg ccggtgtgga cggcgatgcg cagcgtctcg
     2821 gtcagctctc ctggctgctg tttttgaaaa tcttcgatgc ccaggaagag gcgctggaac
     2881 tggagcagga taactatcaa tatccgatcc cacagcgtta tttgtggcgc agttgggccg
     2941 ccaacgcgca gggcattacc ggtgattccc tgctggaatt cgttaatgat gatctgtttc
     3001 cggcgttgaa aaacctcact gcgcctatcg ataaaaaccc acgcggctac gtggtgaagc
     3061 aggcgttcag cgatgcctat aactatatga aaaacggtac gctactgcgc caggtgatca
     3121 acaagctgaa cgaaattgac tttaccagcg ccagcgaacg ccatctgttt ggtgatattt
     3181 acgaacagat ccttaaagat ctgcaatctg cgggcaatgc gggcgaattc tatactccac
     3241 gcgccgtcac tcgctttatg gtggatcgcg ttgatccgaa actcggcgaa tccattatgg
     3301 acccggcctg cggtacgggc ggttttcttg cctgcgcgtt cgatcatgta aagaacaaat
     3361 acgtgaagag cgtcgccgat catcagacac tgcaacaaca gatccacggt gttgagaaaa
     3421 aacagcttcc gcacctgctg gcgaccacca atatgctgct gcacggcatt gaagtaccgg
     3481 tacaaattcg tcacgacaac accctgaata aaccgctttc ctcctgggat gagcaactgg
     3541 atgtcattgt taccaacccg ccgtttggtg gcacggaaga agacggtatt gagaagaact
     3601 ttccggcaga gatgcaaacc cgcgaaacgg cggatctgtt tctgcaactg attgtggaag
     3661 tactggcgaa aaacggtcgt gcggcggtgg tattgccgga tggcacacta tttggcgaag
     3721 gtgttaaaac caaaatcaaa aagctactta ccgaagagtg caacctgcat accatcgtgc
     3781 gtttaccgaa tggtgtgttt aatccctata ccggcattaa aaccaacctg ttgttcttta
     3841 ccaaaggtca gccaaccaaa gagatttggt tctatgaaca tccgtatccg gcgggcgtga
     3901 aaaactacag caaaaccaag ccgatgaagt ttgaagagtt tcaggcggag atcgactggt
     3961 ggggtaacga ggccgatggt tttgccagcc gcgtcgagaa tgagcaagcg tggaaagtca
     4021 gcattgatga cgtgattgcg cgcaacttca atctggatat taaaaaccca catcaggcgg
     4081 aaaccgtcag ccatgatccg gacgaactgt tagcgcagta tgcaaaacag caggcggaga
     4141 tccagacgct gcgtaatcaa ctgcgcgata ttcttggcgc tgcgctgtct ggcaaggagg
     4201 ttaactaatg agtgtggaaa agctgatcgt tgatcatatg gaaacctgga cctcggcgtt
     4261 gcaaacccgt tccaccgccg ggcgcggcag ctccggtaag attgatttgt atggtattaa
     4321 gaaattgcgt gagctgattc tggaactggc ggtgcgcggt aaactggtgc cgcaggatcc
     4381 gaatgatgaa ccggcgtcgg acctgctgaa gcgtattgcg gcggaaaaag cagagctggt
     4441 gaagcaaggc aaaattaaaa agcaaaaacc actgccagaa attagtgagg aagagaagcc
     4501 gtttgaattg ccggagggat gggagtgggt gcacctccct gatatttatt gctcaatttc
     4561 tgagtcatct agaaaaataa aatcctccga aatactacct gagggaaaat atcctgttat
     4621 cgagcaaagc caagaattta tttctggtta ttgcaacaat gaatgtctgt taataaagtt
     4681 aaataatcca gttatagttt ttggtgatca cacaaggaat attaaattta ttgattttga
     4741 ttttgttgtc ggcgctgacg gtgtgaagat tttatcacct atattaattt gtgagcgttt
     4801 ctttttttgg cagttacgat catttaaatt agatgtaaga ggatacgcga gacattttaa
     4861 agtattaaat agctgcttat ttgcattacc ccccattgca gaacaagagc gaatagttga
     4921 aaaagttagc tctttgatgt ccctctgcga ccaactagaa cagcaatccc tgaccagtct
     4981 ggacgcacat cagcaactgg ttgaaaccct gttgggaacg cttacagaca gccaaaacac
     5041 cgcggaactg gctgaaaact gggcgcgtat tagcgagcat ttcgacaccc tatttaccac
     5101 cgaagccagc gtggatgcgt taaaacagac cattctgcaa ctggccgtaa tgggcaaact
     5161 tgtgccgcag gatccgaatg acgaacccgc ctctgaactg ctcaaacgta ttgcgcagga
     5221 aaaagctcaa ttggtgaaag aagggaaaat aaaaaaacaa aaaccactgc cgccaattag
     5281 cgatgaggaa aaaccgtttg aacttccgga agggtgggaa tggtgtcgta ttggtaatat
     5341 agttaatatt aaatctgaat tagtatcacc aaaagattat ttaaatttat atcaagtagc
     5401 acctgatatt attgaaaagg gcactgggag agtgatatca aagagaacag tgaaagaatc
     5461 aggagtcaaa gggccaaata gtcgctttta taaaggacaa attgtttatt ctaaaatccg
     5521 cccgtcatta tccaaagttt ttttagcaga gtataatggt ctttgcagtg ctgatatgta
     5581 tccattagac tgttacatta atccaaatta cttattgaaa tatatccttt ccattccatt
     5641 tcttatgcaa gtcaaaaaag cagagaacag aataaaaatg ccaaaactta attctgacag
     5701 tttttacaat attattgttg cgattccccc ctataatgaa caacaagcaa tatttgataa
     5761 gataaattct attgaagcag tatgcaatgg tcttatttct tatattggga tttatcataa
     5821 aacccaactg catcttgcag acgcactcac tgacgcagcg ataaactaat tccctcgccc
     5881 tcacaaaacc aataaagcgg ctaaatagcc gctttattca catcaccaaa atttatattt
     5941 cacgctaatt ttcatcttat tttcattaat ttttagctgg actttccctg tatttactga
     6001 tgatgtatcc aggtatttag cacggtgcgg atgtgcgcca acacactcgc accgctaatc
     6061 acaatcacta tttatagaga atagtaatca tgactgacac gcattctatt gcacaaccgt
     6121 tagaagcaga agtctccccg gcaaataatc gtcaattaac cgtgagttat gcgagtcgtt
     6181 acccggatta cagccgtatt cccgccatca ccctgaaagg tcagtggctg gaagccgccg
     6241 gttttgccac cggcacggcg gtagatgtca aagtgatgga aggctgtatt gtcctcaccg
     6301 cccaacccgc cgccgccgaa gagagcgaac tgatgcagtc actacgcaag gtgtgtaagc
     6361 tgtcggcgcg taaacaaagg caggtacagg aatttattgg ggtaattaca ggtaaacaga
     6421 aagtcgcctg atacattgac ccggtagcgc cagtgctacc gggtattatt cgccgcgtta
     6481 taccaacgcg ctctccatct gtttttcaaa tgcctgacga attaatccca taatgtaagg
     6541 aagttgtgcg aggtcgctga aaccaatttc tacatcgcca ttaccccagc gcccaacatt
     6601 tgtcacatct ttggcaatac ctttcggatc gaccagttca tgaaactgca tattaagcga
     6661 caatcgcagt cggctttttt gcggcacaac atcaacaaaa ttcgtttccg ctttaaacgc
     6721 aatatatagc ttcaatactt cctgcgtgat ccctgcgtcc aggcgcataa cttcatcgcg
     6781 aagatgatcg aataa
//
LOCUS       DAJTRC010000161         6682 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.161, whole genome shotgun sequence.
ACCESSION   DAJTRC010000161 DAJTRC010000000
VERSION     DAJTRC010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6682)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6682)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6682
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.161"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..1399
                     /gene="sbcB"
                     /locus_tag="OO357_003794"
     CDS             <1..1399
                     /gene="sbcB"
                     /locus_tag="OO357_003794"
                     /EC_number="3.1.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="exodeoxyribonuclease I"
                     /protein_id="HCR5888574.1"
                     /translation="FLFHDYETFGTHPALDRPAQFAAIRTDDEFNVIGEPEVFYCKPA
                     DDYLPRPGAVLITGITPQEARAKGENEAAFAARIHSLFTVPKTCILGYNNVRFDDEVT
                     RNVFYRNFYDPYAWSWQHDNSRWDLLDVMRACYALRPEGINWPENDDGLPSFRLEHLT
                     KANGIEHSNAHDAMADVYATIAMAKLVKTRQPRLFDYLFTHRNKHKLMALIDVPQMKP
                     LAHVSGMFGAWRGNTSWVAPLAWHPENRNAVIMADLAGDISPLLELDIDTLRERLYTA
                     KADLGDNAAVPVKLVHINKCPVLAQANTLRPEDADRLGINRQHCLDNLKILRENPQVR
                     EKVVAIFAEAEPFTPSDNVDAQLYNGFFSDADRAAMKIVLETEPRNLPALDITFVDKR
                     IEKLLFNYRARNFPGTLDYAEQQRWLEHRHQVFTPEFLQGYADELQMLVQQYADDKEK
                     VALLKALWQYAEEIV"
     gene            complement(1442..1669)
                     /locus_tag="OO357_003795"
     CDS             complement(1442..1669)
                     /locus_tag="OO357_003795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HCR5888575.1"
                     /translation="MAIKKLDVVTQVCPFPLIEAKAALAEMASGDELVIEFDCTQATE
                     AIPQWAAEEGHAITDYQQIGDAAWSITVQKA"
     gene            complement(1683..2741)
                     /gene="yeeE"
                     /locus_tag="OO357_003796"
     CDS             complement(1683..2741)
                     /gene="yeeE"
                     /locus_tag="OO357_003796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transport protein YeeE"
                     /protein_id="HCR5888576.1"
                     /translation="MFSMILSGLICGALLGFVMQRGRFCLTGGFRDMYIAKNNRMFYA
                     LLIAISAQSVGVFALIQAGLLTYEAGAFPWLGTVIGGYLFGLGIVLAGGCATGTWYRA
                     GEGLIGSWIALFTYMVMSAVMRSPHASGLNQTLQHYTTEHNSIAETFNLSVWPLVTVL
                     LVITLWVVMKELKKPKLKVATLPPRRTGIAHILFEKRWHPFVTAVLIGLIALLAWPLS
                     EATGRMFGLGVTSPTANILQFLVAGDVKYINWGVFLVLGIFVEAFIAAKASREFRVRA
                     ADAQTTLRSGLGGVLMGFGASIAGGCSIGNGLVMTAMMTWQGWIGLVFMILGVWTASW
                     LVYVRPQRKARLATAAAN"
     gene            complement(2920..4278)
                     /gene="plaP"
                     /locus_tag="OO357_003797"
     CDS             complement(2920..4278)
                     /gene="plaP"
                     /locus_tag="OO357_003797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putrescine/proton symporter PlaP"
                     /protein_id="HCR5888577.1"
                     /translation="MSHNVTPNTSRVELRKTLTLVPVVMMGLAYMQPMTLFDTFGIVS
                     GLTDGHVPTAYAFALIAILFTALSYGKLVRRYPSAGSAYTYAQKSISPTVGFMVGWSS
                     LLDYLFAPMINILLAKIYFEALVPSIPSWMFVVALVAFMTAFNLRSLKSVANVNTVIV
                     VLQVVLIAVILGMVVYGVFEGEGAGTLASTRPFWSGDAHVIPMITGATILCFSFTGFD
                     GISNLSEETKDAERVIPRAIFLTALIGGMIFIFATYFLQLYFPDISRFKDPDASQPEI
                     MLYVAGKAFQVGALIFSTITVLASGMAAHAGVARLMYVMGRDGVFPKSFFGYVHPKWR
                     TPAMNIILVGAIALLAINFDLVMATALINFGALVAFTFVNLSVISQFWIREKRNKTLK
                     DHFQYLFLPMCGALTVGALWVNLEESSMVLGLIWAAIGLIYLACVTKSFRNPVPQYED
                     VA"
     gene            complement(4268..4330)
                     /gene="yoeI"
                     /locus_tag="OO357_003798"
     CDS             complement(4268..4330)
                     /gene="yoeI"
                     /locus_tag="OO357_003798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein YoeI"
                     /protein_id="HCR5888578.1"
                     /translation="MGQFFAYATVITVKENNHVA"
     gene            complement(4491..5474)
                     /locus_tag="OO357_003799"
     CDS             complement(4491..5474)
                     /locus_tag="OO357_003799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HCR5888579.1"
                     /translation="MKPLLDVLMILDAIEKEGSFAAASAKLYKTPSALSYTVHKLESD
                     LNIQLLDRSGHRAKFTRTGKMLLEKGREVLHTVRELEKQAIKLHEGWENELVIGVDDT
                     FPFSLLAPLIEAFYQHHSVTRLKFINGVLGGSWDALTQGRADIIVGAMHEPPSSSEFG
                     FSRLGDLEQVFAVAPHHPLAQEEEPLNRRIIKRYRAIVVGDTAQAGASTASQLLDEQE
                     AITVFDFKTKLELQISGLGCGYLPRYLAQRFLDSGALIEKKVVAQTLFEPVWIGWNEQ
                     TAGLASGWWRDEILANSAIAGVYAKSDDGKSAIQRKIIMTSLSFSCHFPPI"
     gene            complement(5520..6344)
                     /locus_tag="OO357_003800"
     CDS             complement(5520..6344)
                     /locus_tag="OO357_003800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HCR5888580.1"
                     /translation="MKKVAIVGLGWLGMPLAMSLSARGWQVTGSKTTQDGVEAARMSG
                     IDSYLLRMEPELVCDSDDLDALMDADALVITLPARRSGPGDEFYLQAVQELVDSALAH
                     RIPRIIFTSSTSVYGDAQGTVKETTPRNPVTNSGRVLEELEDWLHNLPGTSVDILRLA
                     GLVGPGRHPGRFFAGKTAPDGEHGVNLVHLEDVIGAITLLLQAPKGGHIYNICAPAHP
                     ARNVFYPQMARLLGLQPPQFRNSLDSGKGKIIDGSRICNELGFEYQYPDPLVMPLE"
     gene            complement(6427..6681)
                     /gene="yoeB"
                     /locus_tag="OO357_003801"
     CDS             complement(6427..6681)
                     /gene="yoeB"
                     /locus_tag="OO357_003801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     toxin YoeB"
                     /protein_id="HCR5888581.1"
                     /translation="MKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGK
                     PEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAACRYHY"
BASE COUNT         1793 a         1760 c         1571 g         1558 t
ORIGIN      
        1 cttcctcttt cacgattacg aaacctttgg cacgcacccc gcgttagatc gccctgcaca
       61 gttcgcagcc attcgcaccg atgacgaatt caatgtcatc ggcgaacccg aagtctttta
      121 ctgcaagccc gcggatgact atttaccccg gcctggagca gtattaatta ccggtattac
      181 cccgcaggag gcacgggcga aaggagaaaa cgaagccgcg tttgccgccc gtattcactc
      241 gctttttacc gtaccgaaga cctgtattct gggctacaac aatgtgcgtt tcgacgacga
      301 agtcacacgc aacgtttttt atcgtaattt ctacgatcct tacgcctgga gctggcagca
      361 tgataactcg cgctgggatt tactggatgt tatgcgtgcc tgttatgccc tgcgcccgga
      421 aggaataaac tggcctgaaa atgatgacgg tctaccgagc tttcgccttg agcatttaac
      481 caaagcgaat ggtattgaac atagcaacgc ccacgatgcg atggctgatg tgtacgccac
      541 tattgcgatg gcgaaactgg taaaaacgcg tcagccacgc ctgtttgatt atctctttac
      601 ccatcgtaat aaacacaaac tgatggcgtt gattgatgtt ccgcagatga aacccctggc
      661 tcacgtttcc ggaatgtttg gggcatggcg tggcaatacc agctgggtgg caccgctggc
      721 gtggcatcct gaaaatcgca atgccgtaat tatggcggat ttggcaggag acatttcgcc
      781 attactggag ctggatatcg acacattgcg cgagcgttta tataccgcaa aagccgatct
      841 tggcgataac gccgccgttc cggttaagct ggtgcatatc aataaatgtc cggtactggc
      901 ccaggcgaat acgctacgcc cggaagatgc cgaccgactg ggaattaatc gtcagcattg
      961 cctcgataac ctaaaaattc tgcgtgaaaa tccgcaagtg cgtgaaaaag tggtagcgat
     1021 attcgcggaa gccgaaccgt ttacaccttc agataatgtg gatgcacagc tttataatgg
     1081 ctttttcagt gacgccgatc gtgcagcaat gaaaattgtg ctggaaaccg agccgcgtaa
     1141 tttaccggca ctggatatca cttttgttga taaacgaatt gaaaagttgt tgttcaatta
     1201 tcgggcacgc aacttcccgg ggacgctgga ttatgccgag cagcaacgct ggctggagca
     1261 ccgccaccag gtcttcacgc cagagttttt gcagggttat gctgatgaat tgcagatgct
     1321 ggtacaacaa tatgccgatg acaaagagaa agtggcgctg ttaaaagcac tttggcagta
     1381 cgcggaagag atcgtctaat ttgaagccgc cgccgctggt acggcggagg acaatgttga
     1441 gtcaggcttt ttgaacggtg atgctccacg ctgcatcgcc aatctgctga taatcggtga
     1501 tggcatgtcc ttcttctgcc gcccactgtg ggattgcttc agttgcctgg gtgcaatcaa
     1561 actcaattac caactcatcg ccgctggcca tttctgccag tgctgctttt gcttctatga
     1621 gtggaaacgg acagacctgc gtcaccacat ccagcttttt aatcgccatc tttacacctt
     1681 acttaatttg ccgcagcagt tgccagtcgc gccttacgct gcggtcgaac atacacaagc
     1741 caggacgcag tccagactcc gagaatcata aatacaaggc caatccagcc ctgccaggtc
     1801 atcattgcag tcataaccag tccattaccg atagagcaac cacctgcaat actggcaccg
     1861 aagcccatca gtacaccacc taacccgcta cgtaatgttg tttgtgcatc agctgcgcga
     1921 acgcggaact cacggctcgc tttggctgca ataaatgcct ccacgaagat ccctaacacc
     1981 aggaaaacgc cccagttaat gtatttcacg tcacccgcga ccagaaattg aagaatattg
     2041 gccgttgggg aagtgactcc taacccaaac atgcgtccgg ttgcttcact caggggccag
     2101 gctaaaagcg caatcaaacc gatgagtaca gcagtgacaa agggatgcca gcgtttttca
     2161 aacagaatat gagcgatccc ggttcggcgc ggcggtaagg tcgcgacttt gagttttggc
     2221 ttcttcaact ctttcatcac cacccagagc gttatcacca gcaaaacggt aaccaacggc
     2281 cacacagaca aattaaaagt ctcagcaata gagttatgtt cagtagtgta gtgctgcaag
     2341 gtttgattta aaccactggc gtgtggagaa cgcatcaccg cactcatcac catataagtg
     2401 aaaagcgcga tccaactgcc gatcaatccc tcacccgcgc gataccaggt cccggtggca
     2461 catccacccg ccagaacaat tcccagcccg aaaagatacc cacctataac agtaccaagc
     2521 cacgggaacg ccccggcttc gtaagtcagt aggcccgcct gaattaacgc aaaaaccccc
     2581 acgctttgtg ccgaaattgc aatcagcaag gcgtaaaaca tgcgattatt tttcgcgata
     2641 tacatatcgc gaaaaccacc agtcaggcaa aaacgcccac gctgcatgac aaatcccagc
     2701 agagcaccac aaattagccc gcttaatatc attgaaaaca taaattaatt aaccagatga
     2761 atgttaatgg ggaaattttt catgactggt ggaatgcaga ccaataacca aattctctaa
     2821 attagaacaa atggttatta atgaggattt cgataaaaaa gaataaaaaa accggagcca
     2881 aatgttcatt cgactccggt ttattattag aaaagatgat tatgctacgt cttcgtactg
     2941 cggaaccgga ttacggaagc tcttggtgac gcaagccagg taaatcagac cgatagcagc
     3001 ccagatcaga cccagaacca ttgagctttc ttccaggtta acccacagcg caccaacggt
     3061 cagcgcacca cacatcggca ggaacagata ctggaagtga tctttcagcg tcttgttacg
     3121 cttctcacgg atccagaact gcgagatgac cgacaggtta acaaaggtga acgccaccag
     3181 cgcaccaaag ttaatcagcg ccgttgccat taccaggtcg aagttgattg ccagcagggc
     3241 aatcgcgcca accaggatga tgttcatcgc cggagtacgc catttcgggt gtacataacc
     3301 gaagaagctt ttcgggaaca cgccgtcacg acccattacg tacatcagac gcgctacgcc
     3361 tgcgtgcgcc gccataccgg acgccagtac ggtaatggtg gagaagatca gcgcgccaac
     3421 ctggaacgct ttacccgcaa cgtacagcat gatttcaggc tgtgacgcat ccggatcttt
     3481 aaagcgagag atatccggga agtacagctg caggaagtaa gttgcaaaga tgaagatcat
     3541 gccgccaatc agcgcggtca ggaaaatcgc acgcgggatc acgcgctctg catctttggt
     3601 ttcttccgac agattgctga tgccgtcaaa gccggtaaag gagaagcaca ggattgtcgc
     3661 cccggtaatc atcgggataa catgcgcatc gccagaccag aacggacgcg tgctcgccag
     3721 cgtaccagcg ccttcacctt caaatacgcc ataaacaacc atgcccagaa tcaccgcgat
     3781 cagcactacc tgcaacacga cgattacagt gttgacgttc gctacggatt tcagactacg
     3841 caggttaaag gcggtcataa aggccaccag cgccaccaca aacatccacg atggaatgga
     3901 aggcaccaga gcttcaaaat agattttcgc cagcagaatg ttgatcatcg gcgcgaacag
     3961 atagtcgagc agagaagacc aacccaccat aaagccgaca gtcgggctaa tggatttctg
     4021 ggcgtaagtg tatgcagagc cagcagaagg atagcggcga accagcttcc cgtagctcag
     4081 agccgtaaac aggatcgcaa tcaatgcgaa cgcataggct gtcggcacat gaccatccgt
     4141 gaggcctgaa acgataccaa atgtatcaaa cagcgtcatc ggctgcatat aggctagacc
     4201 catcattaca accggaacta acgtaagcgt tttacgcaat tccacgcgag aggtgtttgg
     4261 agtaacgtta tgcgacatgg ttattctcct ttacggtgat aaccgtcgcg taagcaaaaa
     4321 attgccccat ttttttggat tcctcagcga caacaactgt cgatttttag taaatatcta
     4381 tccggtacga agcccggcct cttggtatga atgattggtt tgaagcaaaa aataaccgac
     4441 gctaattaaa cgtcggtttt taatcatttt ttgacagcgg cgcattgtgc ctaaatgggg
     4501 gggaaatgac aagagaatga gaggcttgtc ataataattt ttctttgaat ggctgatttt
     4561 ccgtcatcag attttgcata aacaccggcg atcgcactat ttgctaaaat ttcatctcgc
     4621 caccagccgc tggcaagccc tgcggtctgt tcattccagc caatccagac aggttcaaag
     4681 agagtttggg cgaccacttt cttctcgatt aacgcgccac tatcgagaaa acgttgcgcc
     4741 agataccgtg gtaaatagcc gcagccaagg ccgctaattt gcagctccag tttggtttta
     4801 aaatcgaaaa cggtaatggc ttcttgttcg tcgagaagtt gtgaggccgt agaagcgcct
     4861 gcctgcgcag tatcccccac cacaatcgcc cgatatcgtt taatgatacg acggtttaac
     4921 ggttcttctt cctgggctaa cggatgatga ggcgcgaccg caaagacttg ttcaagatcg
     4981 cccaaccgcg agaaaccaaa ctcactggaa gatggtggct catgcatcgc gccaacgata
     5041 atatccgctc gcccctgagt cagggcatcc caggaaccac cgagtacgcc attgataaac
     5101 ttcaggcgcg tcacgctatg atgttgataa aaagcttcaa tgagaggcgc aagcagagaa
     5161 aaaggaaacg tgtcgtccac gccaatcacc agctcgtttt cccagccttc atgcagtttg
     5221 atcgcctgct tttccagttc tcgcacggta tgcagaactt cccgcccttt ctctaataac
     5281 atttttccgg tgcgggtgaa tttagcgcgg tgtccgctac gatcaagcaa ttgaatattt
     5341 aggtcgcttt ccagcttgtg aacggtgtaa ctcagagcgg acggcgtctt gtagagtttg
     5401 gctgacgccg ccgcaaaact gccctctttt tctatggcat cgaggatcat caacacatcc
     5461 agcagtggtt tcatacacgc ccccttgcag tgcatgcggc gtcctgctgg cgtgatcgtt
     5521 tactccaacg gcattaccag cggatcggga tactggtatt caaatcccag ttcattacaa
     5581 atccgactgc catcaataat cttacctttg ccgctgtcca gactatttct gaactgcgga
     5641 ggttgcagcc ccagtaaacg ggccatctgc ggatagaaaa cattacgcgc agggtgagct
     5701 ggcgcacata tattatagat gtgtccgcct ttaggtgcct gtaacaacag agtgatagcg
     5761 ccaataacat cttctaaatg gactaaatta acaccatgtt caccatcagg ggcggttttt
     5821 ccggcaaaga agcgtcccgg atgacgtccc ggtccgacca ggcccgcaag acgcagaata
     5881 tcgaccgaag taccgggtaa attgtgcagc cagtcttcga gttcttctaa tactcgtcca
     5941 ctgttggtta ccggattacg cggggtagtt tctttaaccg tgccttgcgc gtcgccatag
     6001 acagatgttg agctggtaaa aataattcgg ggaatacgat gggccagcgc gctatccact
     6061 aactcttgta ccgcttgtaa atagaactca tcgccggggc cgctacgacg tgccggaagc
     6121 gtaatgacca gcgcatcggc atccataagg gcatccagat cgtcagaatc gcaaactaac
     6181 tcaggctcca tgcgaagcag atagctatca atgccactca ttcgggccgc ttcgacacca
     6241 tcttgtgtgg ttttactccc ggtgacttgc cagcctcgcg ctgaaagtga catcgccagc
     6301 ggcataccta accaccctaa cccgacaatt gcgacctttt tcatccgtta tctcctgata
     6361 caccttagat ctataaggct acgctagcgt atcaaaactg acaattcatt ctatgaatga
     6421 atctgttcaa taatgataac gacatgctgc aatgagcagt gaatcatcag taaccgcgta
     6481 taccagacgg tgctcctctg taatgcgtcg ggaccagaaa cctgacaaat tatgtttcag
     6541 gggttctggt ttccccttac cttcaaatgg tgttctgcgg gtatctttga taagttcatt
     6601 gatcttttta acaattcgct tatctgtttc ctgccagtac agataatcat cccatgattc
     6661 ctcagaccag attagtttca ct
//
LOCUS       DAJTRC010000162         6521 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.162, whole genome shotgun sequence.
ACCESSION   DAJTRC010000162 DAJTRC010000000
VERSION     DAJTRC010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6521)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6521)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6521
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.162"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            439..1827
                     /gene="narU"
                     /locus_tag="OO357_003802"
     CDS             439..1827
                     /gene="narU"
                     /locus_tag="OO357_003802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/nitrite transporter NarU"
                     /protein_id="HCR5888582.1"
                     /translation="MALQNEKNSRYLLRDWKPENPAFWENKGKHIARRNLWISVSCLL
                     LAFCVWMLFSAVTVNLNKIGFNFTTDQLFLLTALPSVSGALLRVPYSFMVPIFGGRRW
                     PVFSTAILIIPCVWLGIAVQNPNTPFGIFIVIALLCGFAGANFASSMGNISFFFPKAK
                     QGSALGINGGLGNLGVSVMQLVAPLVIFVPVFAFLGVNGVPQADGSVMSLANAAWIWV
                     PLLAIATIAAWSGMNDIASSRASIADQLPVLQRLHLWLLSLLYLATFGSFIGFSAGFA
                     MLAKTQFPDVNILRLAFFGPFIGAIARSVGGAISDKFGGVRVTLINFIFMAIFSALLF
                     LTLPGTGSGNFIAFYAVFMGLFLTAGLGSGSTFQMIAVIFRQITIYRVKMKGGSDEQA
                     QREAVTETAATLGFISAIGAVGGFFIPQAFGMSLNMTGSPVGAMKVFLIFYIVCVLLT
                     WLVYGRRKFSQK"
     gene            1909..5649
                     /gene="narZ"
                     /locus_tag="OO357_003803"
                     /pseudo
     CDS             1909..5649
                     /gene="narZ"
                     /locus_tag="OO357_003803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415985.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase Z subunit alpha"
     gene            5646..>6521
                     /gene="narH"
                     /locus_tag="OO357_003804"
     CDS             5646..>6521
                     /gene="narH"
                     /locus_tag="OO357_003804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate reductase subunit beta"
                     /protein_id="HCR5888583.1"
                     /translation="MKIRSQVGMVLNLDKCIGCHTCSVTCNNVWTGREGMEYAWFNNV
                     ETKPGIGYPKNWEDQEEWQGGWVRDVNGKIRPRLGSKMGVITKIFANPVVPQQIDDYY
                     EPFTFDYEHLHSTPEGKHIPTARPRSLIDGKRMDKVIWGPNWEELLGGEFEKRARDRN
                     FEAMQKEMYGQFENTFMMYLPRLCEHCLNPSCVATCPSGAIYKREEDGIVLIDQDKCR
                     GWRLCISGCPYKKIYFNWKSGKSEKCIFCYPRIESGQPTVCSETCVGRIRYLGVLLYD
                     ADRIEEASSTEREVDL"
BASE COUNT         1558 a         1714 c         1690 g         1559 t
ORIGIN      
        1 cttagatgaa ataaacaata cttactataa taacgattat tacaatcagg caataagaga
       61 ggaaccgata gactttctgg acagatcgtt ttcctccaat tcatggcctt tttagtgaat
      121 aaatcatcac taattctata agtgtctggt cttgttgaga atatagttaa tatgtaaccc
      181 tttagattca cgatctgaag ggttgaaaca tgacatacgt tcaaagatta acccccgttt
      241 gaaggttagc ttgttagtta catttagtaa cacatatctg gctacttaat ccccattctg
      301 cccccgcggt tacgctttct tatctattct ttcaatcagc acttcaccca attcatcctt
      361 aattctaata actggctatt gcgccttacc tcctgttttg tcttttttca ttaccaatgt
      421 gtgcatatga ggaacaatat ggcactgcaa aatgagaaaa atagtcgtta tcttttacgc
      481 gactggaaac cagaaaatcc ggccttctgg gaaaataaag gaaaacatat tgctcgaaga
      541 aacctctgga tatcagtcag ttgtctactt cttgccttct gtgtctggat gctatttagc
      601 gcagttaccg ttaatctcaa taaaatcggt tttaatttta ctaccgatca actcttttta
      661 ttaaccgcat taccctccgt ttctggcgca ttattgcgtg ttccctactc ctttatggtg
      721 cctatattcg gtggacgccg atggccggtt tttagtactg caatcctgat tattccttgc
      781 gtctggctcg gaattgccgt gcaaaatccg aatactcctt ttgggatatt tatcgttatc
      841 gctttgctat gcggttttgc aggtgcaaac tttgcttcga gcatgggcaa tatcagtttc
      901 ttctttccaa aagccaaaca agggagcgct cttgggatta atggcggatt aggaaactta
      961 ggtgtaagtg taatgcagct ggttgcaccg ctggtcattt ttgtacctgt atttgccttt
     1021 ctcggcgtca atggcgtacc gcaggccgac ggttcggtga tgtcgctggc gaatgccgca
     1081 tggatttggg taccgctact ggcgattgcc acgatcgccg cctggtcagg gatgaatgat
     1141 atcgccagtt cacgcgcctc aattgccgac cagctccctg tcttacaacg cctgcatctc
     1201 tggctgctga gcctgcttta ccttgccacc ttcggttcgt ttatcggttt ttctgcgggt
     1261 tttgccatgc tggcaaaaac ccagttcccg gatgtgaata ttctgcgcct ggcgttcttt
     1321 ggcccattta ttggtgccat cgcgcggtcg gttggtggtg ctatttccga taaattcggc
     1381 ggcgtgcggg tgacgttgat caactttatt tttatggcga ttttcagtgc cctgctgttc
     1441 cttaccttac cgggcacagg ctccggtaat ttcatcgcct tttacgccgt atttatgggg
     1501 ctgtttctga ccgcgggtct gggaagtggt tctactttcc agatgatcgc cgtcatcttt
     1561 cgccagataa ccatttatcg ggtgaagatg aaaggcggta gtgatgagca agctcagaga
     1621 gaagccgtca ccgaaacggc ggcaacgctg ggctttatct cagccattgg cgcagtgggc
     1681 ggctttttta ttccgcaggc atttggcatg tcgctcaata tgaccggctc tccggtcggc
     1741 gcgatgaaag tgtttttaat cttctacatc gtttgtgtgc tgctgacctg gctggtttat
     1801 ggtcggcgga agttcagcca aaaataagag atcgatgcct gatgtacaac catgtcgcat
     1861 ccgacaaggc atcagatccc aattcattcc tggagcagga gtcatgtcat gagtaaactt
     1921 ttggatcgct ttcgctactt caaacaaaag ggcgaaacct ttgccgatgg tcacggacag
     1981 gtgatgcata gcaaccgcga ctgggaggac agctatcgcc agcgttggca gttcgacaaa
     2041 atcgtgcgtt ccacccacgg cgttaactgt acaggctcct gtagctggaa aatctacgtt
     2101 aaaaatggtc tggtgacctg ggaaatccaa cagaccgact acccgcgcac tcgccctgac
     2161 ctgcccaatc atgaacctcg cggctgcccg cgtggcgcaa gttactcctg gtatctttac
     2221 agcgctaacc gcctgaaata cccgctcatt cgtaaacgac tgattgaact gtggcgcgaa
     2281 gccctcaaac aacacagcga tccggtactg gcgtgggcat cgattatgaa tgatccgcaa
     2341 aagtgcctga gctacaaaca agtgcgtggg cgcggcgggt ttatccgctc caactggcag
     2401 gaactaaacc agctgattgc cgccgctaac gtctggacca tcaaaaccta cggcccggat
     2461 cgcgttgccg gtttctcgcc gatcccggcg atgtcgatgg tttcttacgc cgccggaacg
     2521 cgttatctgt cgctgcttgg cggcacctgt taaagtttct acgactggta ttgcgacctg
     2581 ccgcccgcct cgccgatgac ctggggcgag caaaccgacg taccggaatc tgccgactgg
     2641 tataactcca gctacatcat cgcttggggg tctaacgtac cgcagacacg tacgccggac
     2701 gcccacttct ttaccgaagt acgctacaaa ggcactaaaa ccatcgccat tacccctgac
     2761 tactctgaag tggccaaatt gtgcgaccag tggctggcac cgaaacaagg cactgatagc
     2821 gccctggcga tggcaatggg gcatgtgatt ttaaaagagt ttcatctcga taatcccagc
     2881 gactacttta tcaactactg ccgccgctac agcgacatgc cgatgctggt aatgctggag
     2941 cctcgcgacg atggtagcta cgttcccggg cgcatgatcc gcgcatctga cctggtggat
     3001 ggactgggcg aaagcaacaa tccgcagtgg aaaaccgtag cagttaatac cgcaggtgaa
     3061 ttggtagtgc cgaacggttc gattggtttc cgctggggag aaaaaggcaa atggaatctg
     3121 gaatccattg ccgccggtac ggaaaccgaa ttgtcgttaa ccctgctcgg tcaacatgac
     3181 gctgttgcag gcgtggcctt cccctacttt ggcggcattg aaaatccgca ttttcgcagc
     3241 gtaaaacaca atccggtgct ggtgcgccaa ttgcccgtta aaaacctgac gttagtcgat
     3301 ggcaacacct gtccggtggt cagcgtttat gatttggtac tggcgaatta cggcctcgat
     3361 cgcgggctgg aagatgaaaa cagcgcgaaa gattacgctg aaatcaaacc gtacacccca
     3421 gcctggggtg agcaaattac cggcgtaccg cgccagtata ttgaaaccat cgcccgcgaa
     3481 tttgccgata ccgcccataa aacgcatggg cgctcaatga ttatcctcgg cgcaggtgtt
     3541 aaccactggt atcacatgga catgaactac cgcgggatga tcaatatgct gatcttctgc
     3601 ggttgtgtcg ggcaaagcgg tggcggctgg gcacactatg tcggccagga aaaactgcgc
     3661 ccacaaaccg gctggttgcc actggccttt gcgctcgact ggaaccgacc accgcgccag
     3721 atgaacagca cctcgttttt ctacaatcat tccagccaat ggcgctatga aaaagtctct
     3781 gctcaggagt tactttcacc gctcgccgat gccagtaagt acagcggtca tctgattgat
     3841 ttcaacgttc gcgccgaacg tatgggctgg ctaccttctg cgccgcagct ggggcgtaac
     3901 ccgctcggga ttaaagctga agccgacaag gccggattat cccccacaga atttaccgcc
     3961 caggcgctga aatcgggcga tttacgtatg gcctgcgaac aaccagatag cggcagcaat
     4021 catccgcgta atttgtttgt ctggcgctct aacctgcttg actcctccgg caaaggccac
     4081 gagtatatac agaagtatct gctggggacc gaaagcggga ttcagggcga ggaactcggt
     4141 gccagcgacg ggatcaaacc ggaagaagtc gagtggcaaa ctgcggcgat tgaaggcaag
     4201 ctcgacctgc tggtgacgct cgacttccgc atgtccagta cctgcctgtt ctccgatatc
     4261 gttctgccca ccgccacctg gtatgaaaaa gacgatatga acacctcgga tatgcatcca
     4321 tttattcatc cgctttctgc ggcggtcgat ccggcctggg aatcacgcag cgactgggaa
     4381 atctacaaag gtattgccaa agcattttcg caagtgtgcg tgggccatct tggcaaagaa
     4441 accgacgtgg tattacaacc actgctgcat gactctccgg cagagctctc acagccgtgt
     4501 gaagtgctcg actggcgcaa aggcgaatgc gatctgatcc cgggtaaaac cgcgccgaat
     4561 attgtggcgg tggagcgcga ctaccctgct acgtatgaac gctttacctc gctcgggcca
     4621 ttgatggaca aacttggcaa tggcggtaaa gggatttcgt ggaataccca ggatgaaatc
     4681 gatttcctcg gcaaactcaa ttacaccaag cgtgatggcc cggcacaggg gcgtccgctg
     4741 attgacaccg ccattgacgc ttcagaagtg attctggcac tggcaccgga aaccaacggt
     4801 catgttgcag tcaaagcgtg gcaggcgctg ggcgagatca ccgggcgcga acatacccat
     4861 ctggcgctgc acaaagagga cgagaagatt cgctttcgcg atattcaggc gcagccgcgt
     4921 aaaattatct ccagccccac atggtccggt ctggaaagcg atcacgtatc ctataacgcg
     4981 ggatacacca acgttcatga gttaattccg tggcgcacgc tgtcgggacg ccagcagctc
     5041 tatcaggatc atccgtggat gcgtgctttt ggtgaaagcc tggtggctta tcgcccgcct
     5101 atcgacaccc gtagcgtcag tgagatgcgc cagataccgc caaacggctt ccaggaaaaa
     5161 gcacttaact tcctgacgcc gcaccagaaa tggggcattc actcaaccta cagtgaaaac
     5221 ctgctaatgc tgacgctctc tcgcggtgga ccgattgtct ggatcagcga aacagatgcc
     5281 cgagaactga ccattgtcga taacgactgg gtggaagtat ttaacgccaa cggcgcgctg
     5341 actgcccgcg cggtggtcag ccaacgtgta ccgccgggca tgaccatgat gtatcacgcc
     5401 caggaacgca ttatgaatat tcctggttcg gaagtaactg gcatgcgcgg cggcattcat
     5461 aactcggtca cccgcgtttg cccgaaaccg acgcatatga ttggcggtta cgcgcagctg
     5521 gcctggggct ttaactacta cggcaccgtc ggttcgaacc gcgacgaatt catcatgatc
     5581 cgcaagatga agaacgttaa ctggctggat gatgaaggtc gcgatcaggt acaggaggcg
     5641 aaaaaatgaa aatacgttcg caagtcggca tggtgcttaa cctcgataaa tgtatcggct
     5701 gccatacctg ttcggtgacc tgtaacaacg tctggaccgg acgcgaaggc atggagtacg
     5761 catggtttaa caacgtcgaa accaaaccgg gcattggtta tccgaaaaac tgggaagatc
     5821 aggaagagtg gcaaggcggc tgggtccgcg atgtgaatgg caagatccgt ccgcgtctgg
     5881 gcagcaagat gggcgtaata accaaaatct tcgccaaccc ggtggtgccg cagcagattg
     5941 atgattacta cgaacctttc accttcgact acgaacattt gcatagcaca ccggaaggca
     6001 aacatatccc tactgctcgc ccgcgttcac tgattgacgg caaacggatg gacaaagtga
     6061 tctggggccc aaactgggaa gaactgctgg gcggcgagtt tgaaaaacgt gcccgcgacc
     6121 gcaacttcga ggccatgcaa aaggagatgt acgggcagtt tgaaaacacc ttcatgatgt
     6181 acctgccgcg cctgtgcgaa cactgcctca atcccagctg cgtggcgacc tgcccaagcg
     6241 gcgctatcta caaacgcgaa gaagacggca ttgtgctgat tgatcaggat aaatgccgtg
     6301 gctggcgttt gtgcattagc ggttgtccgt acaaaaaaat ctacttcaac tggaaaagcg
     6361 gcaagtcaga aaaatgtatc ttctgttatc cgcgaattga gtcaggccaa cccaccgtgt
     6421 gctcagaaac ctgcgtgggt cgcatccggt atctgggcgt gctgctttac gacgccgacc
     6481 gcatcgagga agcgtcgagc accgagcgcg aagttgacct c
//
LOCUS       DAJTRC010000163         6343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.163, whole genome shotgun sequence.
ACCESSION   DAJTRC010000163 DAJTRC010000000
VERSION     DAJTRC010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.163"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..276
                     /locus_tag="OO357_003805"
     CDS             <1..276
                     /locus_tag="OO357_003805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5888584.1"
                     /translation="PAVCFKLKDGEDPGYTLYDLSERLRLRGWQVPAFTLGGEATDIV
                     VMRIMCRRGFEMDFAELLLEDYKASLKYLSDHPKLQGIAQQNSFKHT"
     gene            646..1470
                     /gene="gadX"
                     /locus_tag="OO357_003806"
     CDS             646..1470
                     /gene="gadX"
                     /locus_tag="OO357_003806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadX"
                     /protein_id="HCR5888585.1"
                     /translation="MQSLHGNCLIAYARHKYILTMVNGEYRYFNGGDLVFADASQIRV
                     DKCVENFVLVSRDTLSLFLPMLKEEALNLHAHKKISSLLVHHCSRDIPVFQEVAQLSQ
                     NKNLRYAEMLRKRALIFALLSVFLEDEHFIPLLLNVLQPNMRTRVCTVINNNIAHEWT
                     LARIASELLMSPSLLKKKLREEETSYSQLLTECRMQRALQLIVIHGFSIKRVAVSCGY
                     HSVSYFIYVFRNYYGMTPTEYQERSAQGLPNRDSAASIVAQGNFYGTNRSAEGIRL"
     gene            1838..2566
                     /gene="gadW"
                     /locus_tag="OO357_003807"
     CDS             1838..2566
                     /gene="gadW"
                     /locus_tag="OO357_003807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance transcriptional activator GadW"
                     /protein_id="HCR5888586.1"
                     /translation="MTHVCSVILIRRSFDIYHEQQKISLHNESILLLEKNLADDFAFC
                     SPDTRRLDIDELTVCHYLQNIRQLPRNLGLHSKDRLLINQSPPMPLVTAIFDSFNESG
                     VNSPILSNMLYLSCLSMFSHKKELIPLLFNSISTVSGKVERLISFDIAKRWYLRDIAE
                     RMYTSESLIKKKLQDENTCFSKILLASRMSMARRLLELRQIPLHTIAEKCGYSSTSYF
                     INTFRQYYGVTPHQFAQHSPGTFS"
     gene            complement(2929..6042)
                     /gene="mdtF"
                     /locus_tag="OO357_003808"
                     /pseudo
     CDS             complement(2929..6042)
                     /gene="mdtF"
                     /locus_tag="OO357_003808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417971.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux pump RND permease MdtF"
     gene            complement(6067..>6343)
                     /locus_tag="OO357_003809"
     CDS             complement(6067..>6343)
                     /locus_tag="OO357_003809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5888587.1"
                     /translation="NNFARFILLVPQEGVTHNAQGKATALILDKDDVVQLREIEASKA
                     IGDQWVVTSGLQAGDRVIVSGLQRIRPGIKARAISSSQENASTESKQ"
BASE COUNT         1653 a         1509 c         1511 g         1670 t
ORIGIN      
        1 ccggcggttt gcttcaaact gaaagatggt gaagatccgg gatacaccct gtacgacctc
       61 tctgaacgtc tgcgtctgcg cggctggcag gttccggcct tcactctcgg cggtgaagcc
      121 accgacatcg tggtgatgcg cattatgtgt cgtcgcggct tcgaaatgga ctttgctgaa
      181 ctgttgctgg aagactacaa agcctccctg aaatatctca gcgatcaccc gaaactgcag
      241 ggtattgccc agcagaacag ctttaaacac acctgataac ataacgttgt aaaaaccgaa
      301 tgcccagcct ttaaaaaaac agctgggcat tcggttgctt attaatcgca ataatatatt
      361 ggctgtctat tcatcgtgtt gataagatat atgttatatg aatgtttatt aaatgtagcg
      421 attaccttta atgtcacgaa aagggattat ttgcttgcta ttaatttcac tgcgttctta
      481 aacacgtttt tgtattataa atccagtcat cctgcccgta gaacatcaca cattatcatc
      541 ctgttctccc gcttcgttta aatttattta tcaatcaatt tgacttaaga gggcggcgtg
      601 ctacattaat taacagtaat atgtttatgt aatattaagt caactatgca atcattacat
      661 gggaattgtc taattgcgta cgcaagacat aaatatattc tcaccatggt taatggtgaa
      721 tatcgctatt ttaatggcgg tgacctggtt tttgcggatg caagccaaat tcgagtagat
      781 aagtgtgttg aaaattttgt attagtatca agggatacgc tttcattatt tctgccgatg
      841 ctcaaggagg aggcattaaa tcttcatgca cataaaaaaa tttcttcatt actcgttcat
      901 cactgtagca gagatattcc tgtttttcag gaagttgcgc aactatcgca gaataagaat
      961 cttcgctatg cagaaatgct acgtaaaaga gcattaatct ttgcgttgtt atctgttttt
     1021 cttgaggatg agcactttat accgctgctt ttgaacgttt tgcaaccgaa catgcgaaca
     1081 cgagtttgta cggttatcaa taataatatc gcccatgagt ggacactagc ccgaatcgcc
     1141 agcgagctgt tgatgagtcc aagcctgtta aagaaaaaat tgcgcgaaga agagacatca
     1201 tattcacagt tgcttactga gtgtagaatg caacgtgctt tgcaacttat tgttatacat
     1261 ggcttttcaa ttaagcgagt cgcagtgtcc tgtggatatc acagcgtgtc gtatttcatt
     1321 tacgtctttc gaaattatta tgggatgacg cccacagagt atcaggagcg atcggcgcag
     1381 ggattgccga accgtgactc ggcggcaagt attgttgcgc aagggaattt ttacggcact
     1441 aaccgttctg cggaaggaat aagattatag agttttactc agacataaaa aaaacccggc
     1501 ataggggacc gggaagagga tagtctgccg tctccagact aataaaccgt tataacactc
     1561 cctgttggca cgggaaactt tgtgctctca gtaagttaaa tataactttt acaggaaata
     1621 agatcagcca tttttttata aacataagct atacgctgtg cgaaaatata ttcgtgctgc
     1681 atttacttat tatcaattaa ctgttatgca aaactacttt gtggataaat tttggtccta
     1741 ccaaatctgg cagtttttgc gctaagaaac agtctgtcat catttcatta gtatactgaa
     1801 attgaaataa tcgcagtatg aaatataagg gataatcatg actcatgtct gctcggtgat
     1861 cctcattcgt cgttcattcg atatttatca tgaacagcaa aaaatatcgc tgcataacga
     1921 gagtatcctg ctgctggaga aaaatttggc agacgatttt gcgttttgtt caccggatac
     1981 gcgacgactg gatatcgatg agctgacagt ttgccattac ttacaaaata ttcgtcagct
     2041 accacgcaat ttagggttac atagcaaaga ccgtctgtta attaaccagt caccccccat
     2101 gccgctggtg acggcgattt ttgatagctt caatgaatcc ggggtaaatt caccgatact
     2161 gagcaatatg ctctaccttt cctgtttatc gatgttttct cataagaaag aactgatccc
     2221 cttacttttc aatagcatca gcactgtttc aggaaaagtt gaacgcctta ttagctttga
     2281 tatcgccaaa cgttggtatc tgcgcgatat cgcggaaaga atgtatacca gcgagagtct
     2341 catcaaaaaa aagttgcagg atgaaaatac ctgtttcagt aaaatattac tcgcttccag
     2401 gatgtcgatg gccagacgat tactcgagtt acgtcaaatt cctctgcata ctattgcgga
     2461 aaaatgtggc tatagcagta cgtcgtactt tataaacaca tttcgacaat attatggtgt
     2521 aacgccacat cagtttgcgc aacattcgcc aggtaccttt tcctgacata ttttgcattt
     2581 gaatattggt caggatctca cacctgcttc atgtgaaact cttccctgat gatttctgcc
     2641 gggctaccgg ctagttctct ttcgcagttt gccaggcggt ataggtattt ttaggaggaa
     2701 aagactcggc atgtttggga ttattaagct gacaattcat acgattacgg gaatgtgggt
     2761 cagtattgtg ttgttcaaat tgatgacgaa tggctggagt ggtttctact ttcagtgctg
     2821 tgtactaagc ctggtattcc tgacggttag ctggttgttg agtggtgaat ggttagcagg
     2881 aaagagtaag gctgaacctt cacgttcaac cttactctca tttacacgtt acgctttttt
     2941 aaagcgggca aagagatgtt ccactacaac gaaaaagacc ggaacgaagt aaattgccag
     3001 cactgttgcg gcaaacatcc cgcccatcac gccggtacct accgcgtttt gcgcgccaga
     3061 accggcacca tgactgataa ccagcggcag cacgccgaga ataaaggcca gagaggtcat
     3121 caggattggg cgtaaacgca tccgcgccgc ttcgatgatt gcctctatcg gcgttttccc
     3181 ttctttctgc atcatctcaa cggcaaattc gacaatcagg atggcgttct tggcggaaag
     3241 cccgatggtg gtcagcaaac caacctggaa gtagacgtca ttacttaagc cgcgcagatc
     3301 ggtggccagt aatgcgccaa cgacgcctaa cggaacaacc aacatcaccg agaacggaat
     3361 tgaccagctc tcatagagtg cggcgagggc gaggaacacc acgaccagtg aaatggcata
     3421 cagtgcagga gcctggtttg aggataacgc ttcctgatac gacagtccgg tccatgagta
     3481 gccgacgcct gccggaagtt tagcgaccag gtctgccata aatttcatag cgtcaccggt
     3541 acttttcccg gccgccgctt cacctaaaat ctccattgac gggatgccgt tgtagcgttc
     3601 caatcgcggt gaaccatagg tccattcggt agacgagtag gcagaaagcg gtgccatcgt
     3661 gccagaggcg ttgcgtacat accattggtt gatgttatcc ggcaacatac ggaacggcgt
     3721 gcctgcctgg acatacactt ttttcacccg cccctggttg aggaagtcgt tcacgtagct
     3781 gctgccgaag gcggtggaaa ttgtctggtt gatatcagac agcgccacac ccattgcctc
     3841 agctttcgcg gcgttgacgt tcactttgaa catcggcgta tcttccaggc cgttcgggcg
     3901 taccccggtg acctggtcag gcgattgcgc tgccagtgat aacagctcgt ttcgtgccta
     3961 ggttagtttt tcgtgcccca ggttaccgtt gtccagcagt tccatatcaa aacctgacgc
     4021 ggtacccagt tcagccaccg cgggtaagtt gaacgggaag acgacggctt tattgatact
     4081 gcttaacgca atcattgccc gctgaatgat cgcggtaacc gagttttcct caccgacacg
     4141 ttcagaccac ggcttgagac tgataaacgc caggccgttg ttttgcccct ggccgctgaa
     4201 gccaaagccg ccaacggtaa acaccgactg aacattatct ttctcttttg taagataata
     4261 atccgtcacc tgttgcagta ctttcgtggt gttaaccatg gtggcaccgg aaggtaactg
     4321 cgcggtggtc ataaataccc cctgatcctc ttctggtaag aaagaggtcg gtgtacgcag
     4381 gaacagcacc gccatcccgg cgcaaatcag caggtagacc accatgtagc gaccggtaca
     4441 acgcaacagc gagcgggtgc tatcggtata gtgctgagtt gatttttcaa acagcgtgtt
     4501 gaagcgtgcg aacagggcgt taggtttgtg accgccttcc ggcgtggctt tcagaatggt
     4561 ggcgcacagg gcaggggtca ggctcattgc cacaaatact gaaagccgca tggaggagat
     4621 cagcgtgatg gagaactggc ggtagatctc cccggttgca ccgctcataa aggccatcgg
     4681 cataaacact gcagaaagaa caacggcaat cccgaccagc gcacgttgga tctgccccat
     4741 cgatttatgc gtcgcttcct tcggcggtag cttatcttcc gcaatgacac gctcgacgtt
     4801 ctccaccacc acgatggcgt catccaccag taaccctatc gccagcacca tcccgaacat
     4861 agtcaacgtg ttgatggtga aaccgaccgc cgacaagatc gcaaacgtcc cgagaataac
     4921 caccggtacg gcaattgtcg ggatgatggt ggcacggaaa ttctgcaaaa acagataaat
     4981 gaccaggaag acgaggatga tagcctcaac cagtgttttg aaaacttcct gaatagaaat
     5041 ttcgataaac ggcgtggtgt cgtaaggata aaccgtcttc agacttgcgg ggaaataggc
     5101 tgataagcgg ttcagctcct ctttgactgc ccgcgaggta tccagggcgt ttgctccggc
     5161 agccagtttg atggcgatcc cggcaacagg tttgccgtta tagcgcgcca cggtggaata
     5221 atcttccgcc ccaagttcga cgcgggcgac atcacgcagc agcacttgcg aaccatcttg
     5281 ctgaactttc aacaggattt tgccaaattc ttccggcgtt tgcagacgcg tctgcacaat
     5341 gatcgaggcg tttagttgct ggtctgccgc ctgtggcatg ccacccagtt gaccaccaga
     5401 aatctggttg ttttgcacct taatctggga aataacatcg gaaggtacca ggttatattt
     5461 attgagtttt tgcgggtcca gccagatacg catggcgtac tcggaaccaa agagctgtac
     5521 gctaccaacg cccgcggtac ggcttagcgg gtctttgata ttagacgcta catagtccgc
     5581 gatatcgtac tggttgaggc tgccgttatc agaaataaac gccgctacca tcaggatatt
     5641 actgctcgac ttatcgacgc taatcccctg ttgctgcacc gcttcaggta atgaaggcat
     5701 agcgagttgc agtttatttt gcacctgaac ctgtgcgata tcaggagatg ccccagtctc
     5761 gaaggtcaga gtgatagagg cattacccgc cgcatcactg gttgaagaca tgtacatcag
     5821 gccatcaagc ccattcatat tttgctcaat cacctgagtg accgagtctt ctaccgtttg
     5881 cgcatcggca cccggatagg tagcgctgac ggtaatggtc ggtggcgcaa tctgcggata
     5941 ctgcgcaacc ggtaagttca tgatcgccag accacctgca agcatcataa taatggcaag
     6001 tacccaggca aaaaccgggc gatcaataaa atagttagcc atgaaagtcc ccttaagcct
     6061 gcaacgttat tgtttcgatt cggtgctggc gttttcctgg ctggaggaaa ttgctcgtgc
     6121 tttgataccc ggacgaatgc gttgcaaacc ggaaacgatc acccgatcgc cagcctgcaa
     6181 gccagaggtg acaacccact ggtcgccgat ggctttgctg gcttcaattt cgcgtagctg
     6241 cacgacatcg tctttatcca gaatgagcgc cgttgcttta ccctgggcgt tgtgggtgac
     6301 gccttcctgc ggcaccagta atataaagcg cgcaaaatta ttc
//
LOCUS       DAJTRC010000164         6320 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.164, whole genome shotgun sequence.
ACCESSION   DAJTRC010000164 DAJTRC010000000
VERSION     DAJTRC010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6320)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6320)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6320
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.164"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            73..378
                     /locus_tag="OO357_003810"
     CDS             73..378
                     /locus_tag="OO357_003810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5888588.1"
                     /translation="MIYIEQLELIHKSGDVLYPVKITRKSSGKTAFHLVPFGLNKTHD
                     LLEVEDASEAIRLVIDERHSIRCSTLTATITNKKGKRIKRTGIYSIKGVNIKEYNVR"
     gene            complement(454..918)
                     /locus_tag="OO357_003811"
     CDS             complement(454..918)
                     /locus_tag="OO357_003811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhcH/YjgK/YiaL family protein"
                     /protein_id="HCR5888589.1"
                     /translation="MMMGEVQSLPSAGLHPALQDALTLALAARPQEKAPGRYELQGDN
                     IFMNVMTFNTQSPVEKKAELHEQYIDIQLLLNGEERILFGMAGTARQCEEFHHEDDYQ
                     LCSAIENEQTIILKPGMFAVFMPGEPHKPGCVVGEPGEIKKVVVKVKADLMA"
     gene            complement(915..1790)
                     /gene="nanK"
                     /locus_tag="OO357_003812"
     CDS             complement(915..1790)
                     /gene="nanK"
                     /locus_tag="OO357_003812"
                     /EC_number="2.7.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine kinase"
                     /protein_id="HCR5888590.1"
                     /translation="MTILAIDIGGTKLAAALIGADGQIRDRRELPTPASQTPEALRDA
                     LSALVSPLQAHAQRVAIASTGIIRDGSLLALNPHNLGGLLHFPLVKTLEQLTNLPTIA
                     INDAQAAAWAEYQALEGDITDMVFITVSTGVGGGVVSGGKLLTGPGGLAGHIGHTLAD
                     PHGPVCGCGRTGCVEAIASGRGIAAAAQGELAGADARTIFTRAGQGDEQAQQLIHRSA
                     RTLARLIADIKATTDCQCVVVGGSVGLAEGYLALVEMYLAQEPAAFHVDLLAAHYRHD
                     AGLLGAALLAQGEKL"
     gene            complement(1787..2476)
                     /locus_tag="OO357_003813"
     CDS             complement(1787..2476)
                     /locus_tag="OO357_003813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HCR5888591.1"
                     /translation="MSLLAQLDQKIAANGGLIVSCQPVPDSPLDKPEIVAAMALAAEQ
                     AGAVAIRIEGVANLQATRAVVSVPIIGIVKRDLEDSPVRITAYIEDVDALAQAGADII
                     AIDGTDRPRPVPVETLLARIHHHGLLAMTDCSTPEDGLACQKLGAEIIGTTLSGYTTP
                     ETPEEPDLALVKTLSEAGCRVIAEGRYNTPAQAADAMRHGAWAVTVGSAITRLEHICQ
                     WYNTAMKKAVL"
     gene            complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="OO357_003814"
     CDS             complement(2524..4014)
                     /gene="nanT"
                     /locus_tag="OO357_003814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312124.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sialic acid transporter NanT"
                     /protein_id="HCR5888592.1"
                     /translation="MSTTTQNIPWYRHLNRAQWRAFSAAWLGYLLDGFDFVLIALVLT
                     EVQGEFGLTTVQAASLISAAFISRWFGGLMLGAMGDRYGRRLAMVTSIVLFSAGTLAC
                     GFAPGYITMFIARLVIGMGMAGEYGSSATYVIESWPKHLRNKASGFLISGFSVGAVVA
                     AQVYSLVVPVWGWRALFFIGILPIIFALWLRKNIPEAEDWKEKHAGKAPVRTMVDILY
                     RGEHRIANIVMTLAAATALWFCFAGNLQNAAIVAVLGLLCAAIFISFMVQSTGKRWPT
                     GVMLMVVVLFAFLYSWPIQALLPTYLKTDLAYNPHTVANVLFFSGFGAAVGCCVGGFL
                     GDWLGTRKAYVCSLLASQLLIIPVFAIGGANVWVLGLLLFFQQMLGQGIAGILPKLIG
                     GYFDTDQRAAGLGFTYNVGALGGALAPIIGALIAQRLDLGTALASLSFSLTFVVILLI
                     GLDMPSRVQRWLRPEALRTHDAIDGKPFSGAVPFGSAKNDLVKTKS"
     gene            complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="OO357_003815"
     CDS             complement(4124..5017)
                     /gene="nanA"
                     /locus_tag="OO357_003815"
                     /EC_number="4.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate lyase"
                     /protein_id="HCR5888593.1"
                     /translation="MATNLRGVMAALLTPFDQQQALDKASLRRLVQFNIQQGIDGLYV
                     GGSTGEAFVQSLSEREQVLEIVAEEAKGKIKLIAHVGCVSTAESQQLAASAKRYGFDA
                     VSAVTPFYYPFSFEEHCDHYRAIIDSADGLPMVVYNIPALSGVKLTLDQINTLVTLPG
                     VGALKQTSGDLYQMEQIRREHPDLVLYNGYDEIFASGLLAGADGGIGSTYNIMGWRYQ
                     GIVKALKEGDIQTAQKLQTECNKVIDLLIKTGVFRGLKTVLHYMDVVSVPLCRKPFGP
                     VDEKYLPELKALAQQLMQERG"
     gene            complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="OO357_003816"
     CDS             complement(5139..5930)
                     /gene="nanR"
                     /locus_tag="OO357_003816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417693.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NanR"
                     /protein_id="HCR5888594.1"
                     /translation="MGLMNAFDSQTEDSSPAIGRNLRSRPLARKKLSEMVEEELEQMI
                     RRREFGEGEQLPSERELMAFFNVGRPSVREALAALKRKGLVQINNGERARVSRPSADT
                     IIGELSGMAKDFLSHPGGIAHFEQLRLFFESSLVRYAAEHATDEQIDLLAKALEINSQ
                     SLDNNAAFIRSDVDFHRVLAEIPGNPIFMAIHVALLDWLIAARPTVTDQALHEHNNVS
                     YQQHIAIVDAIRRHDPDEADRALQSHLNSVSATWHAFGQTTNKKK"
BASE COUNT         1610 a         1709 c         1564 g         1437 t
ORIGIN      
        1 atcatcagat ggctgcttaa aaaaagaaca aatggtagtg tccgctattg ccagtacacc
       61 tcaaaagttg ccatgattta cattgaacag cttgaattaa tccataaaag tggtgatgtg
      121 ttgtatccag taaaaataac cagaaaatca tcaggaaaaa ctgccttcca ccttgttcca
      181 ttcggcttaa ataagactca cgaccttcta gaggtggaag atgcttcaga agcaattcgg
      241 ctggtgattg atgaacgtca ttccattcgc tgcagtacac ttacagcaac tattacgaat
      301 aaaaagggca agcgaattaa gcggacaggt atatacagta tcaagggtgt taacataaaa
      361 gaatacaacg tcaggtaatg gcacagagcg gtccgtcagt tcaggtttgg ctctgtgcca
      421 aagatgggtc aggtcacacc tgatctaata aatttaagcc attaaatcag ccttaacctt
      481 caccaccacc tttttaatct cacccggctc gccaacaacg catcccggct tatgcggctc
      541 tcctggcata aacacggcga acattcccgg tttcagaatg atggtttgct cgttttcaat
      601 ggcgctacaa agctggtaat catcttcatg gtggaactct tcacattggc gcgcagtgcc
      661 tgccatgcca aacagaatcc gttcctcacc gtttaataac agctggatat caatgtattg
      721 ctcgtgcaat tccgcttttt tctcgacggg cgattgagtg ttaaacgtca tgacattcat
      781 aaaaatattg tcgccctgta attcgtaacg acccggcgct ttttcttgcg gtctggcagc
      841 taaggccagc gttaacgcgt cctgtaacgc aggatgtaac ccagcagacg gtaatgactg
      901 tacttcaccc atcatcataa tttttctccc tgggccaaca gcgcagcccc aagtaaacct
      961 gcatcatggc ggtaatgcgc cgccagtaaa tcaacatgaa atgccgctgg ctcctgcgcc
     1021 agatacattt ccaccaacgc cagataccct tctgccagac caacgctgcc accaaccacc
     1081 acgcactggc aatcagttgt ggctttaata tcagcgatca gccttgcaag cgtacgtgcg
     1141 gagcgatgaa tcagttgctg cgcctgctca tcgccctgcc cggcgcgtgt gaaaatagtt
     1201 ctcgcatccg cgccagccag ctcgccctgc gctgccgccg caatgccgcg accagaagca
     1261 attgcttcca cgcaacctgt gcgtccacag ccgcagactg ggccgtgtgg atcggcaagc
     1321 gtatgcccga tgtgccccgc cagaccgcca gggccggtaa gcagtttgcc gccgctcact
     1381 acaccgccgc cgacgccagt ggaaacggtg ataaagacca tatcggttat atcgccttcc
     1441 agcgcctgat actctgccca tgctgcggcc tgcgcgtcgt taatggcaat ggtcggcaaa
     1501 ttggtaagtt gttccagcgt tttgactaac ggaaagtgta gcaatccacc gagattatgc
     1561 ggattcagcg ccagcaaact gccgtcgcga ataattccgg ttgaagcgat ggcaacccgc
     1621 tgcgcatgag cttgcaacgg agagactaat gcggataagg catcacgcaa ggcttctggt
     1681 gtctggctgg ctggcgtagg aagttcacga cgatcgcgga tctgcccgtc agcgccaatc
     1741 agcgcggcgg caagtttagt accgccgata tcaatcgcca gtatggtcat agcaccgcct
     1801 ttttcatcgc tgtgttgtac cactgacaaa tgtgttcaag acgcgtaatg gcagaaccga
     1861 ccgtcaccgc ccacgcgccg tggcgcatcg catctgccgc ctgagcaggc gtgttgtaac
     1921 gcccttcggc aatcacccga catccggctt cgctcaacgt tttcaccagc gccagatccg
     1981 gctcttctgg cgtttcaggc gtggtatagc cagaaagcgt agtgccaata atttcggctc
     2041 ccagcttttg gcatgccagg ccgtcttccg gcgttgagca gtcggtcatc gccagtaaac
     2101 cgtgatgatg gatacgtgcc agcagcgttt caacaggcac cggacgcggg cggtcggtgc
     2161 cgtcaatggc gataatgtcc gcgcccgcct gcgccagcgc atcgacatct tcaatatagg
     2221 ccgtgatgcg taccggagaa tcctccagat cgcgtttcac aattccaata atcggcacgc
     2281 tcaccaccgc acgcgtggct tgcagatttg ccacaccttc gatgcgaatg gcaaccgcgc
     2341 ccgcctgctc tgccgctaat gccatggctg cgacgatttc gggtttatcg agcgggctgt
     2401 caggaaccgg ctggcaggag acaatcaggc caccgttagc agcgattttt tgatccagtt
     2461 gtgcaagtaa cgacatacat cttcccttag cgaaaggccc ggtacataga ccgggcaaca
     2521 ggattaactt ttggttttga ctaaatcgtt tttggcgctg ccaaatggca cggcaccgct
     2581 aaatggtttg ccgtcgatag cgtcatgagt acgcaatgct tccgggcgca gccaacgctg
     2641 aacgcgggaa ggcatatcca gaccaatcag caggatcacc acaaatgtca ggctgaacga
     2701 gagcgatgcc agcgcagtac ccagatccag acgttgagcg atcaacgcgc cgatgattgg
     2761 ggccagtgcg ccacccaatg cgccaacgtt gtaggtaaag cccaggcccg ctgcacgctg
     2821 gtcggtatcg aaataaccgc caatcagttt cggtaagatc ccggcgatcc cttgtccaag
     2881 catttgctgg aagaacagta acagaccgag cacccagacg tttgcgccgc caatcgcaaa
     2941 taccggaata atcagcagct gcgaggccag caggctacaa acgtacgctt tgcgggttcc
     3001 cagccagtca ccgaggaagc cacctacgca gcatcccacg gccgcgccaa agccactaaa
     3061 gaacagcaca ttggcgacag tatgcgggtt ataagccaga tcggttttca gatacgttgg
     3121 cagcagcgcc tgaatcggcc atgagtagag gaaagcaaac aacacgacca ccatcagcat
     3181 tacgcccgtt ggccagcgtt tacccgtact ttgcaccata aagctgataa agattgcggc
     3241 gcataacagc ccgagaacag cgacgattgc agcattttgc aggttaccgg cgaagcagaa
     3301 ccacagcgca gtagccgccg ccagtgtcat tacgatattg gcaatgcgat gttcaccacg
     3361 gtagagaata tccaccattg tgcgtactgg tgctttacct gcgtgtttct ctttccagtc
     3421 ttccgcttcc gggatgtttt tacgcagcca gagagcaaag atgattggca aaatgccgat
     3481 aaagaacagc gcacgccaac cccagaccgg aaccaccagg ctatagacct gagcggcaac
     3541 gacggccccc acagagaagc ctgaaatcaa aaaaccactg gctttgttac gcagatgttt
     3601 tggccagctt tcaatgacat aggtggcgct ggaaccgtat tcacccgcca tccccatgcc
     3661 gatgaccaga cgagcgataa acatggtgat gtagcctggc gcaaagccgc aggccagcgt
     3721 cccggccgag aagagaacga tgctggtgac cattgccaga cgacgcccgt agcggtcacc
     3781 catagcgccg agcatcaggc cgccgaacca gcgagagata aaggctgcgg agatcagact
     3841 tgccgcctgc accgtcgtca gcccgaattc accctgtact tcggtgagta ccagggcgat
     3901 taaaacgaaa tcaaaaccgt caagcagata tcccaaccag gcagcggaaa atgcgcgcca
     3961 ttgtgcacgg ttgagatggc gataccacgg gatattctgg gttgtagtac tcattgtgag
     4021 tctcccgcgg tgggcgatgc ccacacgctt tggtatgaaa attgtagggt acagatgcgt
     4081 ttatttcccc tcacccggta ggggcgagcg aggggaaaac aactcacccg cgctcttgca
     4141 tcaactgctg ggccagcgcc ttcagttctg gcagatattt ttcatctacc ggtccaaacg
     4201 gtttgcggca cagcggcaca gaaacgacat ccatataatg caggacagtt ttcaggccgc
     4261 ggaatacgcc cgttttgatc agtaaatcaa tgactttgtt gcactcagtt tgcagtttct
     4321 gcgcggtctg gatatcgcct tctttcagcg ccttcacgat cccctgatat cgccagccca
     4381 tgatgttgta agtactgccg ataccaccat cagcgcctgc cagcaagccg gaagcgaaga
     4441 tttcgtcgta accgttatag agcaccagat caggatgttc acgacggatc tgttccatct
     4501 gatagagatc gccagaagtc tgtttcagcg caccgacgcc aggcaacgta accagggtgt
     4561 tgatctgatc cagggtcagt tttaccccac tcagggctgg aatgttgtac accaccatcg
     4621 gcaaaccatc cgctgaatca ataattgccc gatagtgatc gcagtgttct tcaaagctga
     4681 aaggatagta gaacggcgtg acggcggaga cggcatcgaa gccataacgt ttagccgatg
     4741 ccgcaagttg ttggctttcg gcggtgctga cgcaaccgac gtgggcgatg agtttaatct
     4801 tacctttcgc ctcttcggcg acgatttcca gtacctgttc acgctcggaa aggctttgta
     4861 caaaggcttc gccggtcgaa ccacccacgt ataaaccgtc gatgccctgc tgaatattga
     4921 actgaaccag gcggcgcaga ctcgctttat ccagtgcttg ttgttgatca aaaggagtca
     4981 ggagtgcagc cattacgcca cgtaaattcg ttgccataaa tacctctgaa gtgatgcttg
     5041 tctgataaac gatatacctt tatacctgtt ataccagatc aattaagcaa caccccatac
     5101 agaaagctta taatgcgatc tgcttcacta aagtggcatt atttcttttt gttggtggtc
     5161 tgaccgaaag cgtgccaggt agcagagacg ctgttgagat gcgattgcaa cgcacgatcg
     5221 gcttcgtcag gatcatgacg gcggatcgca tcaacgatcg caatatgctg ttgataacta
     5281 acgttgttat gttcgtgcag tgcctgatcg gtaaccgttg ggcgtgcggc aataagccag
     5341 tcgagcaggg caacgtggat cgccatgaag attgggttac cggggatctc cgccagcacg
     5401 cggtggaaat caacgtctga acgaatgaat gccgcgttgt tatccagcga ctgactgttg
     5461 atttccagtg cttttgccag caaatcgatt tgctcatcgg tggcgtgttc agccgcatag
     5521 cgcaccaggc tggattcaaa gaacagacgt aattgttcga aatgggcaat cccaccagga
     5581 tgagaaagga aatctttcgc catgccggaa agctcgccga tgatagtgtc cgcagaaggt
     5641 cgagagacac gagcgcgttc gccgttgttt atttgcacca gacctttgcg ttttaacgct
     5701 gccagcgctt cacgcacaga aggacgcccg acgttaaaga acgccatcag ttcgcgttca
     5761 gacggtaatt gttcaccttc gccaaattca cgacggcgga tcatctgttc cagctcttct
     5821 tccaccattt cggagagttt tttacgcgcc agcgggcggc tacgcaagtt gcgaccaatt
     5881 gcaggtgaag aatcttcggt ttgcgaatca aatgcgttca taaggcccat tctgtaaggt
     5941 cagtgtgatt aacatcatca gtgacatcct atcacaggat tgaaagtagg ggaaaatggc
     6001 aggtttttct ctttgtgcct catcattacc ataattaacg gaataattaa ctattgcgaa
     6061 aaattaatgt aacgcagata aacacatccc gattgaatta ttcataagac tattcacaag
     6121 cattatgaat attatgaatg tgttcttaca aaataatcat aatcgcatat tttttaatga
     6181 aaaatcacct cacctacaat taaaaacacg acatccgcac cataaataga cttgcaaaaa
     6241 atataacatc attgttttca atctgccatt tatgggattg accgttttct tttgacacgg
     6301 agttcaacaa tgggtaatgc
//
LOCUS       DAJTRC010000165         6200 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.166, whole genome shotgun sequence.
ACCESSION   DAJTRC010000165 DAJTRC010000000
VERSION     DAJTRC010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.166"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO357_003817"
     CDS             complement(85..900)
                     /gene="sul2"
                     /locus_tag="OO357_003817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5888595.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            2089..2238
                     /locus_tag="OO357_003818"
     CDS             2089..2238
                     /locus_tag="OO357_003818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017880354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888596.1"
                     /translation="MPSEYDEQLDSTDTQLPGEYDLFDLMAQVTEENLHAPVDFGSPV
                     GREAL"
     gene            complement(2983..3423)
                     /locus_tag="OO357_003819"
     CDS             complement(2983..3423)
                     /locus_tag="OO357_003819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5888597.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
     gene            3641..4030
                     /locus_tag="OO357_003820"
     CDS             3641..4030
                     /locus_tag="OO357_003820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888598.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4074..4478
                     /locus_tag="OO357_003821"
                     /pseudo
     CDS             4074..4478
                     /locus_tag="OO357_003821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(4585..5421)
                     /locus_tag="OO357_003822"
     CDS             complement(4585..5421)
                     /locus_tag="OO357_003822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5888599.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(5421..>6200)
                     /locus_tag="OO357_003823"
                     /note="aph(3'')-Ib"
     CDS             complement(5421..>6200)
                     /locus_tag="OO357_003823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR5888600.1"
                     /translation="GESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASRRGELAGER
                     DRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAWPSMGQQLG
                     AVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLLARVERELP
                     VRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLALMIANAEE
                     NWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
BASE COUNT         1431 a         1618 c         1641 g         1510 t
ORIGIN      
        1 aaaaaaaata ttagttcgat tcaatggagg ttccttcagt tttctgatga agcgcgaata
       61 tagagaaata tcccgaatgt gcagttaacg aattcttgcg gtttctttca gcgccgccaa
      121 taccgccagc ccgtcgcgca aggggcgcgg ctcgtgtgtg cggatgaagt cagctccacc
      181 tgcggcggcg gcaagctctg cagcgagtgt cgcggccccg acatcccccg gaccacggcc
      241 tgtgagcgcg cgcagaaagg atttgcgcga aacagacaga agcaccggca aatcgaagcg
      301 cagccgcaat tcatcgaacc gcgccagcac cgagagcgag gtttcgggag cagcccccag
      361 aaaaaacccc atgccgggat caaggacaag gcggttgcgt ttgataccgg cacccgtcag
      421 cgccgcgatg cgcgcgtcaa agaacgccgc aatgtgatcc atgatgtcgc cagcgggtgc
      481 ctcgcgccga tctgcctgcc cgtcttgcac cgaatgcata acgacgagtt tggcagatga
      541 tttcgccaat tgcggataga acgcagcgtc tggaaaaccg cgaatatcat tgagataggc
      601 cacaccacgc gacaaggcat aggcttgcgt cgcgggttga taactgtcga gcgagacggg
      661 aatgccatct gccttgagcg cgtccagcac cggcgcgata cgcgcgattt ctgtgtcgga
      721 cgaaacaggc gcggcgtcgg gattgctgga tgccggaccg aggtcgatca catctgcccc
      781 ctcggccatc agcttacgcg cctgcgcaat ggctgcgtct ggcgccagat accggcctcc
      841 atcggagaaa ctgtccgagg ttatgttgac gatgccgaaa atgatgagcg atttattcat
      901 gggggcttct ataataataa taatcgagca tgagtctcat acggatgctc gggtcgaaag
      961 ggaatcccca ggcgagtaac ctgtttgcgg tgatccgttt catgatgatg tatctgtagc
     1021 attttggaaa taaatatatc atctctcaat aagtagatat atttatcttt tttgcttctt
     1081 tggtgctgca agtcaccaat acgagggaaa cctgttacgg agcggtttaa ccgtaatcaa
     1141 cagtgactgg tgtcatgttc ggaagattaa ttgatagtaa tctacaaagg atcttcttga
     1201 gatccttttc aatgaatcgt aatctcttgc tctgtaaacg aacaaaccgc ctggggaggc
     1261 ggtttgttcg aaggttaagt cagttgggga actgcttaac ccggtaacag ggttcacgag
     1321 accgccatta ccaaaactgt tctttcagtg tagccgcagt caggcctcta cttcaagacc
     1381 tcttaatcta tcttctcgtg cactcctgtt actaatggct gctgccagtg gcgttttgtc
     1441 gtgtctttcc gggttggact caagttgata gttaccggac aaggcgcagc ggtcggactg
     1501 aacggggggt tcgtgcatac agtccagctt ggagcgaact gccttcacgg aactgagtgt
     1561 caggcgtgga atgagaaacc gcggccataa cagcggaatg acaccggtaa accgaaaggc
     1621 aggaacagga gagcgcacga gggagctacc agggggaaac gcctgggatc tttatagtcc
     1681 tgtcgggttt cgccaccact gatttgagcg tcagattttg cgatgcttgt caggggggcg
     1741 gagcctatgg aaaaacggct tcgccgtggc cttatctctt ctttgctaag tgtcttcctg
     1801 gtatcttcca ggaaatttcc gtccccgtcc gtaagccggt accgctcgcc gcagccgaac
     1861 gaccgagcgt agcgagtcag tgagcgagga agcggaatat ctcctgtatc gcatattctg
     1921 ctgacgcgcc ggtgctgcct tttttctcct gccacatgaa gcacttcact gattttcgca
     1981 atcgttgcaa catagtaagc cagtatacac tccgctagcg ctacatgact ggttcagggc
     2041 tgcgccccga aacccgctaa caacctctca gctactggat atcaccccat gccatctgaa
     2101 tatgatgaac aactggattc gactgatacg cagcttcccg gcgaatatga cctttttgat
     2161 ttaatggcgc aggttactga agagaacctg catgcccctg tggattttgg gtcgcctgtc
     2221 ggccgggaag cgttatagct ttttcgcttg cccgcgtacg tggtaatact ccggtattac
     2281 cttttatttc tttattttca tgtaattaca gtggattttt aatacccggg tattactcgt
     2341 gaaaacgaaa tattcctttt ttttctttta attcatgcag ttgcgatgaa aatcgcggct
     2401 gcgttaggtg tatgaccatt ttgaggttga aaaataatca accatggcgg gtgatttacg
     2461 caggttaagg ggagatcgtg acaaaaaaag cgttcctcac tgtatcgttc atgcctgcat
     2521 gaacgggtta ttcttcctga ctacagaatt acatcgctcc ttcagttcat acatcacttt
     2581 tacatcactt atacatcact tttacatcat tccttcagct catacgtcac tttcatcctc
     2641 tgtgccattt gtacatcacc cccttcctga acgtgctgac agcgggtttt ccggctgcgc
     2701 cggcaacgct tcacggccac gcaataaatt gcgtccccgt tcaccgtttt cggatgggaa
     2761 gaacgccgga aacaggactt accccggctg aatggtgtga atttccgcgc tatgcacttg
     2821 tgcgcatact catgcatgcc gtaaaaacag agcctgcgcg tttctggcgg gttttcgggt
     2881 ggtttgttgc ctgttttacc ggtttcccgt cagaaacgcc ctgagggcct ctcaggcggt
     2941 gctgataaca gatgttatgg taaatcagag tcacctgcac tgttactctg aaccggagcg
     3001 aaacagccgc gaccagaatc cccgcttttt ttcctgtgcc agcgcgtcag ccagacggct
     3061 aatttcggcc tgtagttgtt ccctctcctc atttcgcagg cggttttctg cttcccgcgc
     3121 catcttgtct tcaagcatta gggtaacagt ctgctgcaga gcgtccagtt tctgctgaat
     3181 gacgtctagc tgagcatttt ccgcctggct aacgacagat ttggagtgtc cctccttgtg
     3241 cctttttggt gtctcagaag tgcgcagctc gccataggcc cgaatcagtt cactggtctc
     3301 gaactcccgg cgatcatttt ttcctgtgcg atagctgaca aggcctttag ccatgtcacg
     3361 atataattgc gagcgcgact ttcctgttaa tttcagtgtc tcttgaactg tgtgccatgc
     3421 cataaaagtg acagtgtctc ctgtgtgtac cgtattgtaa cagcagaaca tattactgtt
     3481 ttaacacaaa aggtctgagg ctgcccgtga ggcgtgaaaa atacacaaaa aacctgttga
     3541 cggtgtggtt ttgacgggcg tagtatttcg aatactgtat ataaacacat tgttataaat
     3601 acagtttttg ctgtttttaa aagtcaaaga ggataatttt atgagtacag agttaaatag
     3661 cggtactgtc ggtggttttg gtcacatggg accaaatgac ccctacaatg atggtcatag
     3721 cggctctgga agttcgggag gtagttttaa cggtggtggt aatggtggcc atgataatag
     3781 tagtggttca tcttatactg catcctttgg tctcatcact ggccgacacc cgataactgt
     3841 cccaggtacg aagaatatta attctgtcga tctttctgct gtcattaata acattataca
     3901 tcatgaaggt gcacgcaata acatgtacct agatactgcg aacaatgtaa gagtgggagt
     3961 gacgggcact ggctggcaat gtctagcaac ggcaggcatt tcggctgagg gtaaaagaac
     4021 tttccgctaa gcgatagact gtatgtaaac acagtattgc aaggacgcgg aacatgcctc
     4081 atgtggcggc caggacggcc agccgggatc gggatactgg tcgttaccag agccaccgac
     4141 ccgagcaaac ccttctctat cagatcgttg acgagtatta cccggcattc gctgcgctta
     4201 tggcagagca gggaaaggaa ttgccgggct atgtgcaacg ggaatttgaa gaatttctcc
     4261 aatgcgggcg gctggagcat ggctttctac gggttcgctg cgagtcttgc cacgccgagc
     4321 acctggtcgc tttcagctgt aagcgtcgcg gtttctgccc gagctgtggg gcgcggcgga
     4381 tggccgaaag tgccgccttg ctggttgatg aagtactgcc tgaacaaccc atgcgtcagt
     4441 gggtgttgag cttcccgttt cagctgcgtt tcctgtttgg ggtcgtttgc gggaaggggc
     4501 ggaatcctac gctaaggctt tggccagcga tattctccgg tgagattgat gtgttcccag
     4561 gggataggag aagtcgcttg atatctagta tgacgtctgt cgcacctgct tgatcgcggc
     4621 cgcgatagct agatcgcgtt gctcctcttc tccatccgcg ttccaagctg cggaaaggca
     4681 cccataagcg tacgcctggt cgagcaggcg acgcggatcg acgtccagcg cacgagagaa
     4741 tgcgtccgcc atctgtgcaa tgcgtctagg atcgagacaa aggtcgtctc tgtcagccgg
     4801 atcgtagaac atattggcgg cgccaaagcc cacttcaccg accagaccga cgggatctat
     4861 caccagccag ccgcgactgg agaacatgat gttttcatga tgcagatcgc catgtagccc
     4921 acgcagttcc gaggcattgc tcatcatttg atcggctata atcgccgcgt ggacgtagtc
     4981 agtttgacaa cctgcgtttt gatcatcgcg cgcccgctga aacaaagctg caaagcgatc
     5041 ccggatcggg agaagggcag aaggcagggg ttcctcagat gcggcataca gcttcgccat
     5101 tagttccgct gcaatttcgg tcgcctggta gtcgccgtgc tcggcaacga tgtgagagag
     5161 cattcgctcc ccggcatatt cgagcaacat cagattgttc tcacgaccga gcaaccggac
     5221 tgctcccctc ccattgcgcc ataccagata gtcggccccg cgcagttcat cagcaatgtc
     5281 ttctataggt ttcaatccct tgacgattgc aggagtcccg tctggcaatg aaactttcca
     5341 aacgaggctg gaaaaggtgt ccgcaatgag aacaggttgc gaaacgtgcc aatgagcagg
     5401 aaaaacaggc ggcatgaaca tcaaccccaa gtcagagggt ccaatcgcag atagaaggca
     5461 aggcgttcgc ggtcgggggc ttcgatcccc aatacattga ataggacagc gaaggcgcgc
     5521 tctgcttcat ctggcgctgc ccagttctct tcggcgttag caatcatgag tgccaaatcg
     5581 gcatagcgat ctgctgttcc gagccgccca aggtcgatca gacccgtgca ttgaagagtt
     5641 ttagggtcca ccatgaagtt cggcatgcag ggatcaccat ggcaaacaac catatcggtg
     5701 cgctcttggt cgagccgcac cggtagctct cgttcgacac gagccaaaag atcgagctgc
     5761 ggcgtactct tgtcctcgtc cggtaagaag tcgggattga cggcattgcg ggacaccaca
     5821 tcaacggcgc gtccgaacat tcgcgacagc ctgcgctcaa acggacattg atcaaccgat
     5881 aggctgtgaa cagcgccaag ttgctgcccc attgacggcc acgctttgag caaatccgct
     5941 ccagacagat cagccgccgg tactcccgga attgccgtta tcaccaagca tgcaccctcc
     6001 tgttcctcct gccagttgat gacctcgggg caagccacac ctcgaccttt gagccaaatg
     6061 aggcggtcac gctctccagc gagctcaccg cggcgggaag caggtgcgat tttcgcgaag
     6121 gcatgcccgt caccacgtcg aaaaacaaaa tcaccagatt ctccgcctct gacaggcaac
     6181 cagtcagaat gcgattcacc
//
LOCUS       DAJTRC010000166         6174 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.167, whole genome shotgun sequence.
ACCESSION   DAJTRC010000166 DAJTRC010000000
VERSION     DAJTRC010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6174)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6174)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6174
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.167"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            1..163
                     /locus_tag="OO357_003824"
                     /pseudo
     CDS             1..163
                     /locus_tag="OO357_003824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            688..1866
                     /gene="ltrA"
                     /locus_tag="OO357_003825"
     CDS             688..1866
                     /gene="ltrA"
                     /locus_tag="OO357_003825"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005017045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5888601.1"
                     /translation="MCVATREPPGCDGQTLKMFDQQRDGNLYKIWNRLCSGTWFPPPV
                     LEKRIPKSNGKERILGIPTVSDRIAQGAIKLFMEEKLDPIFHADSYGYRPGKSAHDAL
                     KQCAIRCWRYSWILEVDISAFFDHVRHDLVLKALEHHGMPKWVILYCRRWMEAPMQSC
                     ENGELITRTRGTPQGGVISPLLANLFLHYAFDLWMEREYRGVPFERYADDIVVHCSRM
                     SDATRLKNRLSERFSEVGLVLNAGKTNIAYIDTFKRRNVATSFTFLGYDFKVRTLKNF
                     NGERYRKCMPGASNAAMRKITETIKKWRIHRSTAESLLDFARRYNAIVRGWIEYYGKF
                     WSRNFNYRLWSAMQSRLLKWMQSKYRLSNRKAQRKLTLVRKEYPKLFVHWYLLRASNE
                     "
     gene            1949..2128
                     /locus_tag="OO357_003826"
                     /pseudo
     CDS             1949..2128
                     /locus_tag="OO357_003826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(2158..2640)
                     /locus_tag="OO357_003827"
                     /pseudo
     CDS             complement(2158..2640)
                     /locus_tag="OO357_003827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016154415.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            complement(2896..3876)
                     /locus_tag="OO357_003828"
     CDS             complement(2896..3876)
                     /locus_tag="OO357_003828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HCR5888602.1"
                     /translation="MENRKHRPTIGRTLNTNILNNTEEISAPVHVFTLNTGRKAKFTE
                     IKVDHDKVDTQTFVVEEVNGREQTALTPDSLKDITRTIRLQQFYPCIGIRTGDLIEIL
                     DGSRRRAAALLCKVGLRVLVTDDELTVSEAQHLAKDLQTSLEHNIREIGLRLVRLKEA
                     GMNQKQIAEREGLSAAKVTRALQAASVPKDFVSLFPVQSELTYADYRQLAELSERLRL
                     GDISIDEVVKNISPSIELITADDNLSEDEVKNSIMRLITKEMSSLLDSGVKDKAVVTL
                     LWKFDSKDKFARKRVKGRTFSYEFGRLPLEVQDKLDRMIALVLKDNLNSL"
     gene            complement(3876..5075)
                     /locus_tag="OO357_003829"
     CDS             complement(3876..5075)
                     /locus_tag="OO357_003829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HCR5888603.1"
                     /translation="MTSFEQLSKVAQRADKMLLALTKQIQEQKQEFQADVFYQVYSKS
                     AVAKLPKLTRASVDGAVGEMEAQGYQFEKRPAGTATKYALTIQNIIDIYAHRGIPKYR
                     DRYSEAYSIFIGSLKGGVSKTVSSVSVAHALRAHPHLLSEDLRILLLDLDPQSSATMF
                     LNYLHAVGLVDTTAPQAMLQNVSREELLEDFIVPSVIPGVYVMPASIDDAFIASNWDT
                     LCEEHLLGQNKHAILRENIIDKLKHDFDFILIDTGPHLDAFLKNAIAAADIMFTPVPP
                     AQVDFHSTLKYLARLPELVQIIEQDGCSCRLQANIGFMSKLANKSDHKYCHSLTKEIF
                     GGDMLDVSMPRLDGFERSGESFDTVISANPVTYVGSGEALKNARMAAEDFAKAVFDRI
                     EFIRANY"
     gene            complement(5397..5735)
                     /locus_tag="OO357_003830"
                     /pseudo
     CDS             complement(5397..5735)
                     /locus_tag="OO357_003830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            complement(5802..>6174)
                     /gene="istB"
                     /locus_tag="OO357_003831"
     CDS             complement(5802..>6174)
                     /gene="istB"
                     /locus_tag="OO357_003831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS21-like element IS100kyp family helper ATPase
                     IstB"
                     /protein_id="HCR5888604.1"
                     /translation="TAADLLLQLSTAQRQGRYKTTLQRGVMAPRLLIIDEIGYLPFSQ
                     EEAKLFFQVIAKRYEKSAMILTSNLPFGQWDQTFAGDAALTSAMLGRILHHSHVVQIK
                     GESYRLRQKRKAGVIAEANPE"
BASE COUNT         1672 a         1423 c         1473 g         1606 t
ORIGIN      
        1 gctggaaccg ctgtatgaca tcctgcgcca gtatgtgctg atgcccggta aagtccatgc
       61 cgatgatatc cccgttccgg tccaggagcc gggcagcggt aaaacaagga ctgcccggct
      121 gtgggtctac gttcgtgatg accgtaacgc cggttcggaa attgcagcat aagttatcca
      181 tgctctgagg tgggtagagg tgggccctcc ggtcagggtt tacaggagca cagaccaaac
      241 gacccggtgc tgcttcattc taaggaatga ggtggtgagc gactcgggaa aaggcagaga
      301 tctactgtca cgtgggttcg ggaagctgaa cgaaagtaaa ctgtccgatg aagcatcgaa
      361 atcttagatg atgccaaaat ggggccgtac agccgtcccc tgataagcgc agaagacacc
      421 tgcttactga ctgcgcggca tccggtgtga aggcagcagg atcccgggcc agaccatctc
      481 agggaacgtg agaacctgtt tccggcacgt atcgggaaac aggggcggac ccattcgtag
      541 tagtgatgaa agatctgtaa tgggtctgga gcgaagggat ggggatcaca ttcattgcag
      601 aagacacaac ccaaaagggg atgacgtaac tgatgcaacg aaaatcattt gaaataccaa
      661 aagcactggt ctgggcatca tatctggatg tgcgtcgcaa caagggagcc ccccggatgc
      721 gacgggcaga ctttgaaaat gttcgaccag caacgtgatg gcaatctata caagatctgg
      781 aaccggctat gttcgggaac atggtttccg ccaccagtgc tggaaaaacg gatcccgaag
      841 tcgaatggca aggaacgcat tctggggatc ccaacagtat ccgatcgaat tgctcaggga
      901 gcgataaaac ttttcatgga agaaaagctt gacccgattt tccatgctga ttcatatggt
      961 taccgaccag gcaaatcagc tcatgacgct ctgaaacaat gtgccatccg gtgctggcgt
     1021 tatagctgga tactggaagt ggacatcagt gcgtttttcg accatgtgag acatgatctg
     1081 gtgctcaaag ctctggaaca ccacgggatg cctaaatggg tcatcctgta ctgtcgacgc
     1141 tggatggaag cgccaatgca gagttgtgaa aatggagaat taataacccg aacacgaggc
     1201 actccgcagg gcggggtcat tagtccgcta cttgctaact tgttccttca ctatgcattt
     1261 gatttgtgga tggaaagaga atatcggggg gtaccgtttg agaggtacgc tgacgatatt
     1321 gtagtacatt gttcacgaat gagtgatgca acaaggctaa agaacaggtt atcggagcgc
     1381 ttctcggaag tcgggctggt cctgaatgca gggaaaacga atatcgccta cattgacacg
     1441 tttaaaagga gaaacgtcgc aacgagtttc acctttctcg gatatgactt caaagtgcgt
     1501 acgctgaaga atttcaacgg cgaacggtac cgaaaatgca tgccgggtgc gtcaaatgca
     1561 gcaatgcgca aaataacaga aacaatcaag aagtggcgta tacatcgctc aacagctgag
     1621 agtttgctgg attttgcgag gcgctacaat gcgatagtga gaggctggat cgagtactac
     1681 ggaaagttct ggtccagaaa tttcaactat cgactgtgga gtgcaatgca gtcacgtctg
     1741 ctcaagtgga tgcagtctaa atacagactt tcgaaccgga aggctcagcg aaagctgacg
     1801 ctggtaagga aggagtatcc gaagctattt gttcactggt atttactacg tgcatcaaat
     1861 gagtgatcaa gagccgtatg acgcgagagt gtcacgtacg gttccgtgag cagccgggga
     1921 gtgaaattct ctccggctga ctcgactcgc gggttccgac agcgatgacg aacatgcggc
     1981 ggtgttgtac tcgctgatcg gcacatgccg tctgaacaat gtggaaccag aaaaatggct
     2041 gcgttacgtc attgagcata tccaggactg gccggcaaac cgggtacgtg atctgttgcc
     2101 ctggaaagtt gatctgagct ctcagtaaat atcaatacgg ttctgacgag ccgcttactt
     2161 ttggcgtaat tctgctgcgc ctttactgaa cacaatgccg tattcagcaa gaagtccacg
     2221 aatgtgattt atcagttgtg tcctttccat tgaaaccaac cgacgcatat tcaaaaaagt
     2281 ctggacgtcc tgttgctcca gagtttttat tggtacgggt cgtatagttc catgttggca
     2341 ggcctcagct attgcctgtg cgtcgttata atcatttttc tggcaccgct gataagcttt
     2401 aacatgttgt gccggaagca atattacatc atggtcgagt ttcctgattt cccgggccca
     2461 gtaatgagat gccccgccag cctccatcgc aacagtcatt ttaggctgtt gcaccagcca
     2521 gttaagcagt tgattgcgag aaagtttggt ctttcctgcg ggtttgccat gagcattgat
     2581 actgaaaaga taaaagttcg ttttagccag atcaatgccg gtgattttct gttcagacat
     2641 ggtatcccgc ttacttttgt tgttaaagtt tatccatcat ggcgcaattg aaggctgccg
     2701 tgaaagaggg ggaagactat ctcatcggtt actgttaaac ggctaagaga aactatcttt
     2761 ttcaagcaaa gaagatacgg tgaaacaggc gatttcaccg tgaaatactg ttcaaaaaaa
     2821 acgctaataa aagcaaaatt tcttttaaaa cagccaatta aaaactggcg agttgctggc
     2881 gaaaaacttg aattttcaca gcgaattcag attatctttt agtacaagag caatcatacg
     2941 atctaactta tcctgaactt ccaacggtaa tctgccgaat tcatatgaga acgtacgtcc
     3001 ctttactcgt tttcgagcaa atttatcttt actatcaaat ttccataaca aagttacaac
     3061 cgctttatcc tttactcctg aatcaagcaa tgaagacatt tccttggtta taagccgcat
     3121 aatactgttt ttaacctcgt cttcagaaag attgtcatca gcagtaataa gctcaatact
     3181 cggcgagata ttcttcacaa cttcatcaat agatatatca cccagcctca gtcgttcact
     3241 taattcagcc agttgcctgt aatcggcgta tgtcagctca gactgaacag gaaaaagaga
     3301 cacaaaatct ttaggtacgg acgctgcttg gagggcacga gtcaccttag ctgccgacaa
     3361 tccctctctt tccgcaattt gcttctggtt cataccggcc tctttgagac gtactaaacg
     3421 caacccaatt tcacgaatat tatgttcaag tgacgtctgc aaatctttcg ccagatgctg
     3481 agcttcagaa acagtaagct catcatccgt tacaagtaca cgtaatccaa ccttgcatag
     3541 caaagctgct gcccgacgac gggaaccatc caaaatctca ataagatcgc cagtccgaat
     3601 cccaatacat ggatagaatt gttgtaaacg tatagttctg gtgatgtctt ttagtgagtc
     3661 cggcgtcagg gctgtctgtt cgcggccatt cacctcttca accacaaaag tttgagtatc
     3721 cactttgtca tgatcaacct tgatctcagt aaattttgcc tttcggccag tattgagggt
     3781 aaatacatga actggagccg atatttcttc agtattattc aggatatttg tattcagcgt
     3841 acggccaata gtcggtcggt gctttctgtt ttccatcagt agttggccct tataaattca
     3901 atacgatcaa aaactgcttt agcgaaatct tctgctgcca tacgtgcatt tttcaaagct
     3961 tcaccactgc caacataagt aacaggattc gcagatatca cggtatcaaa cgactcgccg
     4021 cttctctcaa aaccatcaag acgtggcatt gaaacatcaa gcatgtcacc accgaaaatc
     4081 tcttttgtca ggctatggca gtatttatga tctgacttat tcgccagttt cgacatgaag
     4141 ccaatatttg cctgcaaacg gcatgaacat ccatcctgtt caataatctg tacaagttca
     4201 ggtaaacgag ccaaatattt aagcgtggaa tggaaatcaa cttgtgctgg cggcaccggt
     4261 gtaaacatga tatctgcggc agcaatggca tttttcaaaa atgcgtccag atgcgggcct
     4321 gtatcaataa ggatgaagtc gaaatcatgc ttcagcttat caataatatt ctcacgcaat
     4381 atcgcgtgtt tgttttgtcc taatagatgc tcctcgcaca gagtatccca gtttgatgca
     4441 ataaaggcgt cgtcaataga agccggcata acataaacgc cagggataac tgacggaacg
     4501 ataaaatctt ccagtaattc ctcacgactc acgttctgca acatagcctg cggtgcggtt
     4561 gtatcaacca gccccaccgc atgcaagtag ttcagaaaca tagtggctga tgactgggga
     4621 tctaaatcaa ggagcagaat acgtaaatct tctgacagca ggtgaggatg agcacgcagg
     4681 gcgtgagcta cggaaacact actcacggtt ttagacacac cgccttttag actaccgatg
     4741 aatatggaat atgcctcact ataacgatcg cgatatttag gaatgccacg atgtgcatag
     4801 atatcgataa tgttctggat ggttaatgca tattttgtcg cggttcctgc tgggcgtttt
     4861 tcaaactgat agccctgagc ctccatctca ccgactgcac catccacgct tgcgcgcgtt
     4921 aattttggaa gcttagctac agcagacttt gaatagactt gataaaaaac atcagcctga
     4981 aactcctgct tttgctcctg aatctgtttt gttagggcga gcaacatttt atctgcacgc
     5041 tgcgcaacct tacttaactg ctcaaaacta gtcattcccc ctccgtgcta tgtaggattt
     5101 taacttatta caaaaaaaac tgcattagca gggtaatgca tgcaagtaca tattgtacat
     5161 gcttatatta aaaatgcgcg cattgctgat ttattaatca tcggaatgaa tattactgta
     5221 agatagtatg cctttccggg agaaagacgc tgatttcccc cgaataatcc agttcaccac
     5281 cacactccgg acaacagctt tcttcaggct ccagatggtg tatttcgcgg gaaacatgtt
     5341 ccggtaacgg acggcgatga cgtgactgtc gcaacaggcg ggggtagtgc ttccaatcag
     5401 tagaacatgt gcttttcaat gaaggagccg atgacttttt cgtggatttc cacggaacgc
     5461 gagaagcaga ttgttttacg tgccagccgc ttgatgcggg tgcggagggt ccggttgtta
     5521 cgtgcaatac gttgcgtgaa gattttgccg gtcaggtgtt tctccttcgg cacttctctg
     5581 gcgtagctgc cccagtcatc gctggtcacc atgccgatac agaacggagt caacagggct
     5641 aacagttccc gacaggtctc atcattccgt ggcccgaaag tgtaagccaa cacaccgcca
     5701 gttttagtgt tgtacgcata ccagagccag tgttgtgtca acgacggatg aaaagtgatc
     5761 cacttatatc tccaccaacg gcccaatatt gatccaccgt tttactcagg attagcttct
     5821 gctataaccc cggcctttcg tttctgtctg agtcgatagc tttctccttt gatttgaacg
     5881 acatgtgagt ggtgtaagat acggcccagc atcgctgagg tcagtgctgc atcaccggcg
     5941 aacgtttgat cccactgccc gaacggtaga ttggatgtca ggatcattgc gctcttttcg
     6001 taacgtttag cgatgacctg gaagaacagt tttgcttctt cctgactgaa cggcagatag
     6061 cctatttcat caatgatgag caggcggggg gccattactc cacgctgaag cgtcgtttta
     6121 taacggccct gacgttgtgc cgtagataac tgaagtaaca gatctgctgc tgtt
//
LOCUS       DAJTRC010000167         6152 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.168, whole genome shotgun sequence.
ACCESSION   DAJTRC010000167 DAJTRC010000000
VERSION     DAJTRC010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6152)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6152)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6152
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.168"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..171
                     /locus_tag="OO357_003832"
     CDS             <1..171
                     /locus_tag="OO357_003832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5888605.1"
                     /translation="IRINFAKKQLEMTNYSVTDIAFEAGYSSPSLFIKTFKKLTSFTP
                     KRYRKKLTEFNQ"
     gene            276..1628
                     /gene="celF"
                     /locus_tag="OO357_003833"
                     /gene_synonym="chbF"
     CDS             276..1628
                     /gene="celF"
                     /locus_tag="OO357_003833"
                     /gene_synonym="chbF"
                     /EC_number="3.2.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HCR5888606.1"
                     /translation="MSQKLKVVTIGGGSSYTPELLEGFIKRYHELPVSELWLVDVEGG
                     KAKLDIIFDLCQRMIDNAGVPMKLYKTLDCREALKDADFVTTQLRVGQLPARELDERI
                     PLSHGYLGQETNGAGGLFKGLRTIPVIFDIVKDVEELCPNAWVINFTNPAGMVTEAVY
                     RHTGFKRFIGVCNIPIGMKMFIHDVLMLKDSDDLSIDLFGLNHMVFIKDVLVNGKSRF
                     AELLDGVASGQLKVSGVKNIFDLPFSEGLIRSLNLLPCSYLLYYFKQKEMLAIEMGEY
                     YKGGARAQVVQKVEKQLFELYKNPELKVKPKELEQRGGAYYSDAACEVINAIYNDKQA
                     EHYVNIPHHGHIDNIPADWAVEMTCKLGRDGATPHPRITHFDDKVMGLIHTIKGFEIA
                     ASNAALSGEFNDVLLALNLSPLVHSDRDAELLAREMILAHEKWLPNFADCIAELKKAH
                     "
     gene            1641..2399
                     /gene="chbG"
                     /locus_tag="OO357_003834"
     CDS             1641..2399
                     /gene="chbG"
                     /locus_tag="OO357_003834"
                     /EC_number="3.5.1.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chitin disaccharide deacetylase"
                     /protein_id="HCR5888607.1"
                     /translation="MERLLIVNADDFGLSKGQNYGIIEACRNGIVTSTTALVNGQAID
                     HAVQLSRDEPSLAIGMHFVLTMGKPLTAMPGLTRDGVLGKWIWQLAEEDALPLEEITQ
                     ELASQYLRFIELFGRKPTHLDSHHHVHMFPQIFPIVARFAAEEGIALRIDRQPLSNAG
                     DLPANLRSSQGFSSAFYGEEISEALFLQVLDDASHRGDPSLEVMCHPAFIDNTIRQSA
                     YCFPRLTELEVLTSASLKYAIAERGYRLGSYLDV"
     gene            complement(2446..4707)
                     /gene="katE"
                     /locus_tag="OO357_003835"
     CDS             complement(2446..4707)
                     /gene="katE"
                     /locus_tag="OO357_003835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707380.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catalase HPII"
                     /protein_id="HCR5888608.1"
                     /translation="MSQHNEKNPHQHQSPLHDSSEAKPGMDSLAPEDGSHRPAAEPTP
                     PGAQPTAPGSLKAPDTRNEKLNSLEDVRKGSENYALTTNQGVRIADDQNSLRAGNRGP
                     TLLEDFILREKITHFDHERIPERIVHARGSAAHGYFQPYKSLSDITKADFLSDPNKIT
                     PVFVRFSTVQGGAGSADTVRDIRGFATKFYTEEGIFDLVGNNTPIFFIQDAHKFPDFV
                     HAVKPEPHWAIPQGQSAHDTFWDYVSLQPETLHNVMWAMSDRGIPRSYRTMEGFGIHT
                     FRLINAEGKATFVRFHWKPLAGKASLVWDEAQKLTGRDPDFHRRELWEAIEAGDFPEY
                     ELGFQLIPEEDEFKFDFDLLDPTKLIPEELVPVQRVGKMVLNRNPDNFFAENEQAAFH
                     PGHIVPGLDFTNDPLLQGRLFSYTDTQISRLGGPNFHEIPINRPTCPYHNFQRDGMHR
                     MGIDTNPANYEPNSINDNWPRETPPGPKRGGFESYQERVEGNKVRERSPSFGEYYSHP
                     RLFWLSQTPFEQRHIVDGFSFELSKVVRPYIRERVVDQLAHIDLTLAQAVAKNLGIEL
                     TDDQLNITPPPDVNGLKKDPSLSLYAIPDGDVKGRVVAILLNDEVRSADLLAILKAMK
                     AKGVHAKLLYSRMGEVTADDGTVLPIAATFAGAPSLTVDAVIVPCGNIADIADNGDAN
                     YYLMEAYKHLKPIALAGDARKFKATIKVADQGEEGIVEADSTDGSFMDELLTLMAEHR
                     VWSRIPKIDKIPA"
     gene            4890..5153
                     /gene="cedA"
                     /locus_tag="OO357_003836"
     CDS             4890..5153
                     /gene="cedA"
                     /locus_tag="OO357_003836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division activator CedA"
                     /protein_id="HCR5888609.1"
                     /translation="MRLVKPVMKKPLRQQNRQIISYVPRTEPAPPEHAIKMDSFRDVW
                     MLRGKYVAFVLMGESFLRSPAFTVPESAQRWANQIRQEGEVTE"
     gene            5430..6152
                     /locus_tag="OO357_003837"
                     /pseudo
     CDS             5430..6152
                     /locus_tag="OO357_003837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707382.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1577 a         1310 c         1557 g         1708 t
ORIGIN      
        1 atccgtatta attttgcaaa aaaacaactg gaaatgacca actattccgt gacggatatt
       61 gcgtttgagg ccggttatag tagcccgagt ttgtttatta aaacgtttaa gaaattaacg
      121 tcctttacgc ctaagagata tcgtaagaaa ttgactgaat ttaatcagta agtcgttata
      181 cctgacaatt cacatatctg tcctgttgct ggattattta tgtccggggg ggcagatatg
      241 ccagatatca gtattctgta ctgaagggag aaattatgag ccagaaatta aaagtcgtca
      301 ctattggtgg cgggagcagc tataccccgg agttactgga aggatttatt aagcgttatc
      361 acgaattgcc ggtcagcgaa ttatggctgg tggatgtcga gggtggcaaa gctaaactgg
      421 atattatttt cgatctctgc caacggatga ttgataacgc tggcgtcccg atgaagcttt
      481 ataaaacgct ggattgccgc gaagcattga aagatgctga tttcgttact acccaactgc
      541 gcgttggcca attaccggcg cgtgaactgg atgaacgtat tccattaagt catggttatc
      601 ttggtcagga aaccaacggc gcgggcggtt tgtttaaagg tctgcgtacc attccggtga
      661 tttttgacat cgtaaaagat gtcgaagaac tttgtccgaa tgcatgggtg attaacttca
      721 ctaacccggc gggaatggtt actgaagccg tttatcgtca taccggattt aaacgcttta
      781 tcggcgtgtg taatattccg atcggcatga agatgtttat tcacgatgtt ctgatgctga
      841 aagacagcga tgatttatct atcgatttgt ttggcctcaa ccatatggtg ttcattaagg
      901 atgtgctggt aaatggcaag tcgcgctttg ccgaattgct tgatggtgtg gcatccgggc
      961 agttaaaagt atctggcgtt aaaaatattt tcgatctgcc atttagcgaa ggcttaattc
     1021 gttctctgaa tctgttgccg tgttcttatt tgctttatta cttcaagcaa aaagagatgc
     1081 tggctattga aatgggcgaa tactacaaag gcggcgcacg agcgcaggta gtacagaaag
     1141 tcgagaaaca actttttgag ctgtataaaa acccggagtt gaaagttaag ccgaaagaac
     1201 tggaacagcg cggtggggct tattactctg atgcagcgtg cgaagtgatc aacgctatct
     1261 acaacgacaa gcaagctgaa cattacgtta atatcccgca tcatgggcat attgataata
     1321 ttccggcaga ctgggcggta gaaatgacct gtaagctggg gcgcgatggc gcgacgccac
     1381 atccgcgcat tacgcatttc gatgataaag tgatggggct gattcacacc attaaaggct
     1441 tcgagattgc tgccagcaac gccgcactta gcggagaatt taacgatgtg ttactggcgc
     1501 taaaccttag tccgttggtg cattccgatc gcgatgctga gctgctggca cgcgagatga
     1561 ttctggcgca cgagaaatgg ctgccaaatt ttgccgactg catcgcagag cttaaaaaag
     1621 cacattaacc gaggctgatt atggaacgct tactgattgt taatgccgat gattttggct
     1681 taagcaaagg tcagaactac ggcattatcg aggcctgtcg caatgggatt gtcacatcga
     1741 cgacggcgct ggtgaatggg caggctattg accatgcggt gcagttgagt cgtgatgaac
     1801 cgagtctggc catagggatg cactttgtcc ttactatggg caagccactg acagctatgc
     1861 cggggttaac ccgcgatggt gtgctgggaa aatggatctg gcagttggca gaagaagatg
     1921 ctttaccgct ggaagaaatt actcaggagc ttgccagtca gtatttgcgt ttcattgagc
     1981 tatttggacg caaacctacg catcttgata gccatcatca tgtgcatatg ttcccgcaga
     2041 ttttcccgat tgttgccagg tttgcggctg aagaggggat tgcgttgcgc atcgaccgtc
     2101 agccgctatc taacgctggt gatttaccgg ctaatctgcg cagttcgcag ggattcagta
     2161 gcgcgtttta tggtgaagag attagcgagg cactgttcct gcaagtgctg gatgacgcaa
     2221 gccatcgggg tgatccttcg ctggaggtga tgtgtcatcc ggcgtttatc gataatacta
     2281 tccgtcagag tgcttactgt ttcccacggt taacggagct ggaagtgctg acatcagcgt
     2341 cgttgaaata tgcaattgct gagcgtggtt atcggttggg tagttatctt gatgtgtaag
     2401 taaggtggta aggtcactcc ccaggggacg tgaccttatc catatttacg ccggaatttt
     2461 gtcaatctta ggaatgcgtg accacacgcg gtgttctgcc atcagcgtta acagttcatc
     2521 cataaaacta ccgtcggtgc tgtcagcttc cacaatccct tcttcaccct ggtcagctac
     2581 cttgattgtt gctttaaact tgcgcgcgtc tcccgccagc gcaatcggtt taaggtgttt
     2641 gtaggcttcc atcagatagt agttggcatc gccgttgtca gcgatatccg cgatattgcc
     2701 gcaagggaca atgaccgcat cgaccgtcag cgaaggtgca ccggcaaagg tagcggctat
     2761 aggcaacacc gtaccgtcat ccgcagtcac ttcacccatt cgggagtaga gcagtttggc
     2821 atgaacgcct ttggccttca tggccttgag aatggccaga aggtctgccg atctcacttc
     2881 atcattaagt aaaatcgcta ccacgcgacc tttcacatca ccgtcaggaa tggcgtacaa
     2941 acttaaggat ggatcctttt tcagaccgtt gacgtccgga ggtggggtga tattcagctg
     3001 gtcgtcagtc agttcgatac cgagattttt cgccaccgcc tgggccagag tgagatcaat
     3061 atgcgccagc tggtcaacaa cgcgctcacg aatatacgga cgaacgactt tgcttaactc
     3121 aaaactgaaa ccatcgacaa tatggcgctg ctcgaatggc gtctgactta gccagaacag
     3181 acgcggatgg gaataatatt cgccaaacga tgggctgcgc tcgcgaactt tattgccttc
     3241 cacgcgctct tggtatgatt caaaaccgcc gcgtttcggc cccggtggtg tttcgcgcgg
     3301 ccagttatcg ttgatcgagt tcggttcata attcgccggg ttagtgtcga tccccatacg
     3361 atgcatgccg tcacgctgga aattatggta agggcaggtc gggcggttaa tcggaatctc
     3421 atggaaattc ggcccaccaa gacgactgat ttgtgtatcg gtataggaga acaaacgtcc
     3481 ctgcaacagg ggatcgttgg tgaagtccag accaggaaca atatgacctg gatggaatgc
     3541 tgcttgttcg ttttcggcaa agaagttatc cggattgcga ttgagcacca ttttgccgac
     3601 acgctgaacg ggcaccagtt cttccgggat cagtttggtt ggatcgagaa gatcgaagtc
     3661 gaacttgaat tcatcttctt caggaatcaa ctggaagccc agttcgtatt ccggaaaatc
     3721 gcctgcttca atggcttccc acaactcgcg gcggtggaag tccgggtcac gtccggtgag
     3781 tttttgtgct tcatcccaaa cgagtgaggc tttacctgcc agtggtttcc agtggaaacg
     3841 tacaaacgtt gccttccctt cggcattaat caggcggaag gtgtgaatac cgaagccttc
     3901 catggtgcgg taactgcggg ggatgccgcg atccgacatc gcccacatca cgttgtgcag
     3961 agtttcaggt tgcagagaaa cataatccca gaaagtatcg tgggcacttt gcccttgtgg
     4021 aattgcccag tgcggttctg gttttaccgc atgaacaaaa tcggggaatt tatgcgcatc
     4081 ctggataaag aagattggcg tgttattgcc aacgaggtca aaaataccct cttcagtata
     4141 gaacttggtg gcaaaaccac ggatatcacg cacggtatcg gcagagccag cgccaccctg
     4201 aacggtagag aacctaacaa atactggggt gattttgttc ggatctgaga ggaaatccgc
     4261 tttggtgata tcgcttaagc ttttatatgg ctggaaataa ccgtgagctg ctgagccgcg
     4321 tgcatgaaca atacgttccg ggatgcgctc atggtcaaag tgggtgattt tctcgcgcag
     4381 aataaaatct tccagcagcg ttggaccacg gttaccggca cgcagtgagt tttgatcatc
     4441 ggcgatgcgc acgccctgat tagtggtcag cgcataattt tcactgcctt tgcgtacgtc
     4501 ttccagagaa ttaagttttt cgttacgtgt atcaggggct ttcaggctcc caggggcggt
     4561 aggttgtgca cctggcggtg ttggttcagc cgctggacga tgagatccgt cctcaggtgc
     4621 cagtgagtcc atccccggtt tcgcttcgct ggaatcgtgt agtggtgact ggtgctgatg
     4681 tgggttcttt tcgttatgtt gcgacattga actcgtctcc ttaatttatt actgaaaggg
     4741 ccgctgtttt ggtcaaaaaa ctctctaact atagaccacc agccagatcc cgcttcggag
     4801 actattctgg aaacggtgtt atgaaataca cgcaaagcgg gacgtacagg ggctaaatcg
     4861 gctaaactac agtttttcta atttttaata tgcgtttagt gaagccagta atgaagaaac
     4921 cgctccgcca gcaaaaccgc cagattatta gctatgtccc acgcacggaa cccgcgccgc
     4981 cagaacatgc gataaagatg gattcgtttc gtgatgtctg gatgctgcgt ggcaaatatg
     5041 ttgcgtttgt actgatggga gagtcatttc tgcgctcacc ggcgtttact gtgcctgaat
     5101 ctgcccaacg ttgggcaaat cagatccgcc aggaagggga agtgactgag taaggattac
     5161 atacctcccg gttttgccgg gagttacagg acctgaaatt ctgcatttcg ttgctacatt
     5221 cattcaaacc acctgtaaca aaaagctgcc tttgacgagc tattgctata aataagagat
     5281 cttttttctt taattattta tttattttaa aatggttaag ttttgctaat catgatgagt
     5341 tatttttgat agttcatcct gtggcgtcgc tcttaaactt atattgtaaa tgacttagat
     5401 ttaaataaga taataatttt gttaacaata tgaggcggga gatgttttct acaaacttaa
     5461 tacagagtaa ttatggtgat ttaaatatta aaagcctggc tttcgactct tttaaagaaa
     5521 ggcttgagtc taccatggcg gcactcactt tctttatatc aacgggtcaa tgtgactgcg
     5581 atgaaatagc agagtcgaac ttcaatcgta tgattgccta tatgtctaac attaactatg
     5641 atgcctcaaa gctaggagcg ccagcactta gttttgatac gtatctgcag gacaatgtta
     5701 aatatagagt gataattaat aatctttatg gcagtgagtt tcggataaga gatattaatg
     5761 agaattttgt tgggatggat tttacctgtg aattccgacc cgaaaaaatg acgaatttga
     5821 tcaatataaa aaataggttg gttattcatt atttaaggac tatatactat gaaaaaaatt
     5881 atatacatcc tgccgggagc atttttgctg cgattcaaaa aaaatgagag tctcctaaag
     5941 tttccttcga taatatcaat gcttaatatc aatcttcttt ttaatccact aaatcttccg
     6001 ggaatgggaa gtggtgtgct tgaagatatt atgagtattc cagatagttc acttagaaaa
     6061 cgtctcgggt atgaggtttt aagcttctca ctacaggcac attcattgag ccaggaatgt
     6121 attgataagt tggacatttt tttcgctgac ga
//
LOCUS       DAJTRC010000168         6052 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.169, whole genome shotgun sequence.
ACCESSION   DAJTRC010000168 DAJTRC010000000
VERSION     DAJTRC010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6052)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6052)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6052
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.169"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            430..1338
                     /locus_tag="OO357_003838"
     CDS             430..1338
                     /locus_tag="OO357_003838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HCR5888610.1"
                     /translation="MKAPVSLTSFRPQKSLAIAIGVLAVVVLPFLSYYTVNEGERGIL
                     LRYGKIVKVAEPGLGFKIPFMESVEKISTRNQAVVYQGLQAYSRDQQPAQMTVSVSFH
                     IKPSEAGAVYTTYNTIEALKDRLIVRQLPTQLENIFGQYTAISAVQDRTKLVQDLQNA
                     MRKAVVGPVVIDGVQIENIDFSDAYEKSIENRMKAEVAIATRKQNLETEKIQAQIAVT
                     QAQAEADSKLAAAKAEAETIRVRGAAEAETIRLKSAAEAEAIRLRGEALRDNPGLVAL
                     TTAERWDGKLPDTMIPGSTVPFISTK"
     gene            complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="OO357_003839"
     CDS             complement(1384..3375)
                     /gene="tkt"
                     /locus_tag="OO357_003839"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708699.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HCR5888611.1"
                     /translation="MSSRKELANAIRALSMDAVQKAKSGHPGAPMGMADIAEVLWRDF
                     LKHNPQNPSWADRDRFVLSNGHGSMLIYSLLHLTGYDLPMEELKNFRQLHSKTPGHPE
                     VGYTAGVETTTGPLGQGIANAVGMAIAEKTLAAQFNRPGHDIVDHYTYAFMGDGCMME
                     GISHEVCSLAGTLKLGKLIAFYDDNGISIDGHVEGWFTDDTAMRFEAYGWHVIRDIDG
                     HDAASIKRAVEEARAVTDKPSLLMCKTIIGFGSPNKAGTHDSHGAPLGDAEIALTREQ
                     LGWKYAPFEIPSEIYAQWDAKEAGQAKESAWNEKFAAYAKAYPQEAAEFTRRMKGEMP
                     SDFDAKAKEFIAKLQANPAKIASRKASQNAIEAFGPLLPEFLGGSADLAPSNLTLWSG
                     SKAINEDAAGNYIHYGVREFGMTAIANGISLHGGFLPYTSTFLMFVEYARNAVRMAAL
                     MKQRQVMVYTHDSIGLGEDGPTHQPVEQVASLRVTPNMSTWRPCDQVESAVAWKYGVE
                     RQDGPTALILSRQNLAQQERTEEQLANIARGGYVLKDCAGQPELIFIATGSEVELAVA
                     AYEKLTAEGVKARVVSMPSTDAFDKQDAAYRESVLPKAVTARVAVEAGIADYWYKYVG
                     LNGAIVGMTTFGESAPAELLFEEFGFTVDNVVAKAKALL"
     gene            3653..4411
                     /gene="loiP"
                     /locus_tag="OO357_003840"
                     /gene_synonym="yggG"
     CDS             3653..4411
                     /gene="loiP"
                     /locus_tag="OO357_003840"
                     /gene_synonym="yggG"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311838.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metalloprotease LoiP"
                     /protein_id="HCR5888612.1"
                     /translation="MKIRALLVAMSVATVLTGCQNMDSNGLLSSGAEAFQAYSLSDAQ
                     VKTLSDQACQEMDSKATIAPANSEYAKRLTTIANALGNNINGQPVNYKVYMAKDVNAF
                     AMANGCIRVYSGLMDMMTDNEVEAVIGHEMGHVALGHVKKGMQVALGTNAVRVAAASA
                     GGIVGSLSQSQLGDLGEKLVNSQFSQRQEAEADDYSYDLLHQRGISPAGLATSFEKLA
                     KLEEGRQSSMFDDHPASAERAQHIRDRISADGIK"
     gene            complement(4617..5537)
                     /gene="speB"
                     /locus_tag="OO357_003841"
     CDS             complement(4617..5537)
                     /gene="speB"
                     /locus_tag="OO357_003841"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HCR5888613.1"
                     /translation="MSTLGHQYDNSLVSNAFGFLRLPMNFQPYDSDADWVITGVPFDM
                     ATSGRAGGRHGPAAIRQVSTNLAWEHNRFPWNFDMRERLNVVDCGDLVYAFGDAREMS
                     EKLQAHAEKLLAAGKRMLSFGGDHFVTLPLLRAHAKHFGKMALVHFDAHTDTYANGCE
                     FDHGTMFYTAPKEGLIDPNHSVQIGIRTEFDKDNGFTVLDACQVNDRSVDDVIAQVKQ
                     IVGDMPVYLTFDIDCLDPAFAPGTGTPVIGGLTSDRAIKLVRGLKDLNIVGMDVVEVA
                     PAYDQSEITALAAATLALEMLYIQAAKKGE"
     gene            complement(5673..>6052)
                     /locus_tag="OO357_003842"
     CDS             complement(5673..>6052)
                     /locus_tag="OO357_003842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888614.1"
                     /translation="GMNYQLYVRNYSRNTNYEIVATADGLDVLNGKQGSLNNNGYIVN
                     AGDSLVIKGFRKDKHTEAAFQFANVADSYAANSAQGDVRNTGVIGFAAFELQGPAQNA
                     LPPCSGQAFPADNNGYAPPPCRK"
BASE COUNT         1491 a         1576 c         1561 g         1424 t
ORIGIN      
        1 ctgaatgtat aactgagatt gatgattctg gaaaataatc actaagccgc ataaattatc
       61 cttattttat ttttagggta ccgtccggcg gtaaaaataa aatactcgtg cggtttaatg
      121 aaagcaattg ggtacgcgag aagaaaaacc ccacaaacca aaataataaa accttaaata
      181 acaacaatat gaaattgata tttttaaagt caactttgta tttgttatgt tcatcacaaa
      241 atcaacattt tgtttgttat tgtatttgtg atgcatatct ctatttattt attgaatgtt
      301 cgaaaacata cttttctaat gcaatgtttt ttcgctatac ccctttatag attctgaatt
      361 ctcacctgtc ggctgttttc tcctcatctc atgtgacata ttcccatact ttactcaagg
      421 aagattgtca tgaaagcacc tgtttcatta acctcatttc gcccacaaaa atctcttgct
      481 atcgccattg gcgtgcttgc cgtggttgtg ctgccatttc tctcgtatta caccgttaat
      541 gaaggtgaac gcggcatttt gctgcgctac ggaaaaatcg taaaagttgc cgaaccgggg
      601 ctgggtttta agatcccttt tatggaaagc gtggagaaaa tttccacgcg taatcaggct
      661 gtggtctatc agggattaca ggcctacagc cgcgaccagc aaccggcaca aatgacagta
      721 tcagtcagct tccacattaa accttcagaa gcaggcgcgg tttataccac ctacaacact
      781 attgaagcat taaaagatcg tctaattgtc cgtcagttgc caactcagct ggagaacatt
      841 ttcggccaat ataccgccat cagcgccgta caggatcgta caaaattggt tcaggatctg
      901 caaaatgcca tgcgcaaagc cgttgttggg ccagtggtaa ttgacggggt ccagattgaa
      961 aatattgatt tttctgacgc ttatgaaaag tcgattgaaa accgaatgaa agcagaagtc
     1021 gccattgcca cgcgtaagca aaatctggaa acagagaaaa tccaggcgca aatagcggta
     1081 actcaggccc aggcagaagc agacagtaaa ctggcggcag ccaaagcaga agcggaaact
     1141 attcgcgtaa gaggcgcggc agaggctgaa actattcgtt taaaaagtgc ggctgaagca
     1201 gaagcaatcc gcctgcgcgg tgaagctctg cgggataatc ccggacttgt agccctcacc
     1261 actgccgaac gttgggacgg taaactgccc gataccatga tccccggcag cacagttccg
     1321 tttatctcta ctaagtaaaa aaagggccgc tttcgcggtc cttctcacaa aacgtcttac
     1381 tgattacagc agcgcttttg ctttcgcaac aacgttatca acagtgaagc cgaactcttc
     1441 aaacagcagc tctgccggag cagattcacc gaaggtggtc atgccgacga tagcgccgtt
     1501 caggccaaca tacttgtacc agtagtcagc aatacccgct tctaccgcaa cgcgtgcagt
     1561 aaccgctttc ggcagtacgg attcacggta agccgcatcc tgcttgtcaa atgcgtcggt
     1621 agacggcatg gacaccacgc gcgctttcac gccttcggca gtcagttttt cgtaggcagc
     1681 aaccgccagt tcaacttcag aaccggtggc gatgaagatc agttccggct gaccagcaca
     1741 gtctttcagc acataaccac cgcgcgcgat gtttgccagc tgctcttcag tacgttcctg
     1801 ctgcgccagg ttctgacggg agaggataag cgcagtcggg ccgtcctgac gctcaacgcc
     1861 gtatttccac gcgaccgcgg attcaacctg gtcacacgga cgccatgtag acatgttcgg
     1921 ggtcacgcgc agagaagcga cctgctctac cggctggtga gtcgggccat cttcgcccag
     1981 accgatggag tcgtgggtgt aaaccatcac ctggcgctgt ttcatcagcg cagccatacg
     2041 tacggcgtta cgtgcgtatt ccacgaacat caggaaggtg gaggtgtacg gcaggaagcc
     2101 accgtgcagg gagataccgt tagcaatcgc ggtcataccg aactcgcgaa caccgtagtg
     2161 gatgtagtta cctgcagcat cttcgttgat tgctttagaa ccagaccaca gggtcaggtt
     2221 agacggtgcc aggtcagcag agccgccgag gaattcaggc aacagcgggc cgaacgcttc
     2281 gatagcattc tgcgacgctt tacggctggc gattttcgcc ggattagcct gcagtttagc
     2341 gataaactct ttcgctttgg cgtcgaagtc agacggcatt tcgcctttca tacggcgggt
     2401 aaattcagcc gcttcctgcg gataagcttt cgcgtaagcc gcaaacttct cattccatgc
     2461 agattctttc gcctggcctg cttctttcgc atcccactga gcatagattt cagacgggat
     2521 ttcgaacggt gcatatttcc agcccagctg ttcgcgggtc agggcaattt cagcgtcgcc
     2581 cagcggcgca ccgtgggagt cgtgagtacc ggctttgttc ggggaaccga aaccgatgat
     2641 ggttttgcac atcagcaggg acggtttgtc agtcactgcg cgcgcttctt caactgcgcg
     2701 tttgatggat gccgcgtcat gaccgtcgat gtcgcgaata acgtgccagc cgtaagcttc
     2761 gaaacgcatt gctgtgtcgt cggtgaacca gccttcaacg tgaccatcga tagaaatacc
     2821 gttgtcatcg tagaacgcaa tcagtttacc cagcttcagc gtacccgcca gagagcaaac
     2881 ttcgtgggag atgccttcca tcatgcagcc gtcgcccatg aaggcgtagg tgtagtggtc
     2941 gacaatgtcg tggcccggac ggttaaactg cgccgccagc gttttttctg caatcgccat
     3001 accgactgcg ttggcaatac cctgacccag cggaccggtg gtggtttcca caccagcggt
     3061 ataacctact tccggatggc ccggagtttt agagtgcagc tgacggaagt ttttcagttc
     3121 ttccatcggc agatcgtaac cggtgaggtg cagcaggctg tagatcagca tggagccgtg
     3181 gccgttggac agcacgaagc ggtcacggtc agcccaggac ggattctgcg ggttgtgttt
     3241 caggaaatca cgccacagga cttcggcaat gtcagccata cccatagggg cacccgggtg
     3301 accggatttg gctttctgta ctgcgtccat gctcagcgca cgaatagcat tggcaagctc
     3361 tttacgtgag gacattttga ctccagatcg gatgatgaag ggcacgccct taacgacttg
     3421 acgacagcgc gttttgggct acgccggaaa atttgccaac aatttaccgc aagccgcgcg
     3481 tcatgtacat ggaacatcct tttgccgctt cagaaatctc tggatcatgc tcgcatgttg
     3541 cgcaatctgc tcgcccgtcc gctgcgcttt tccttatact gagactgagc gtcgattcac
     3601 ctgcaaacgg cgcattttta gaataatcct gaccttgtgc ggaagagaaa acatgaaaat
     3661 tcgcgcctta ttggtagcaa tgagcgtggc aacggtactg accggttgcc agaatatgga
     3721 ctccaacgga ctgctctcat caggagcgga agcttttcag gcttacagtt tgagtgatgc
     3781 gcaggtgaaa accctgagcg atcaggcatg tcaggagatg gacagcaagg cgacgattgc
     3841 gccagccaat agcgaatacg ctaaacgtct gacaactatt gccaatgcgc taggcaacaa
     3901 tatcaacggt cagccggtga attacaaagt gtatatggcg aaggatgtga acgcctttgc
     3961 aatggctaac ggctgtatcc gcgtctatag cgggctgatg gatatgatga cagataacga
     4021 agtcgaagcg gtgatcggtc acgagatggg gcacgtggcg ttaggccatg tgaagaaagg
     4081 gatgcaggtg gcgcttggta caaatgccgt gcgagtagct gcggcctctg cgggcgggat
     4141 tgtcggcagt ttatctcaat cacaacttgg tgatctgggc gagaaattag tcaattcgca
     4201 attctcccag cgccaggaag cagaagccga tgattattct tacgatcttc tgcaccaacg
     4261 cggcatcagc ccggcaggtc ttgccaccag ctttgaaaaa ctggcgaagc tggaagaagg
     4321 tcgtcaaagc tcaatgtttg acgaccatcc tgcatccgcc gaacgtgccc agcatattcg
     4381 cgatcgcatc agcgcggatg ggattaagta aagcctagtg gtgtcgaacg taggtcagat
     4441 aaggcgttca cgccgcatct gacattaatg gcacatttgt taccttgtgc gcattgccgg
     4501 atgcgatgct ggcgcatctt atccggccta cgggtgccga acgtaggtcg gataaggcgt
     4561 tcacgccgca tccgacatta atggcacgtt ttacccgtgc gcatcgcatc tggtgcttac
     4621 tcgccctttt tcgccgcctg aatatacagc atctccagcg ccagcgtcgc cgcagccaga
     4681 gcggtgattt ccgactgatc gtatgccgga gccacttcca ctacgtccat cccaacgatg
     4741 ttgagatctt tcaggccgcg taccagttta atggcgcgat cggaggtcag gccgccaatc
     4801 actggcgtac cggttcccgg cgcgaaagca ggatccaggc agtcgatatc aaaagtcagg
     4861 taaaccggca tatcacccac aatctgtttc acctgggcga taacgtcatc cacgctgcga
     4921 tcgttcacct ggcaggcgtc cagcacggta aagccgttgt ctttatcaaa ctcggtacga
     4981 ataccaatct gcacggaatg attcgggtcg atcagacctt ctttcggcgc ggtatagaac
     5041 atagtgccgt ggtcaaattc acaaccgttc gcataggtat cggtgtgggc gtcaaagtgt
     5101 accagcgcca ttttgccgaa atgcttcgca tgagcacgca gcagcggcag cgtaacaaag
     5161 tggtcaccac cgaaggagag catacgctta ccggcagcca gcagcttctc ggcgtgcgcc
     5221 tgcagctttt cgctcatctc acgggcatcg ccaaaggcat ataccagatc gccgcagtcc
     5281 acgacgttca gacgctcacg catgtcgaaa ttccacggga agcggttgtg ttcccaggcc
     5341 agattcgtcg aaacctgacg gatcgctgcc ggaccgtggc gaccacccgc acgaccagaa
     5401 gtggccatat cgaacggcac gccagtaatc acccagtctg catcgctgtc atacggctgg
     5461 aagttcatcg gcaggcgtaa aaaaccaaag gcattggaaa ccagtgagtt atcgtattga
     5521 tgacctaagg tgctcatgga tggactccta ttacaaagtc gatataaaaa aacccttccg
     5581 cgtcgttagg cccgacgagg aagggttgga tttgcgaaac acaagtttgt gctaattatc
     5641 gccttaaatg ggcgaggcat caagcgaggt tgctatttcc gacacggtgg cggcgcatag
     5701 ccattgttat ctgccggaaa ggcttgtcca gagcacggcg gcagcgcgtt ttgcgccggg
     5761 ccttgtaatt caaaggcggc aaagccgatt accccggtat tacgcacatc gccttgcgcc
     5821 gaattggctg cgtaggaatc agcaacgttg gcgaactgaa aagccgcttc agtatgttta
     5881 tctttgcgga atcctttaat cactaacgaa tctccggcat tcacaatata gccgttgtta
     5941 ttcagcgaac cttgcttgcc gttcagcaca tccaggccat cagccgtagc aacgatctcg
     6001 tagttggtgt tacgactgta attacggaca tataattgat aattcattcc ga
//
LOCUS       DAJTRC010000169         5983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.170, whole genome shotgun sequence.
ACCESSION   DAJTRC010000169 DAJTRC010000000
VERSION     DAJTRC010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.170"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(1..393)
                     /locus_tag="OO357_003843"
                     /pseudo
     CDS             complement(1..393)
                     /locus_tag="OO357_003843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_151035092.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
     gene            complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO357_003844"
                     /gene_synonym="virG"
     CDS             complement(579..3887)
                     /gene="icsA"
                     /locus_tag="OO357_003844"
                     /gene_synonym="virG"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane autotransporter IcsA"
                     /protein_id="HCR5888615.1"
                     /translation="MNQIHKFFCNMTQCSQGGAGELPTVKEKTCKLSFSPFVVGASLL
                     LGGPIAFATPLSGTQELHFSEDNYEKLLTPVDGLSPLGAGEDGMDAWYITSSNPSHAS
                     RTKLRINSDIMISAGHGGAGDNNDGNSCGGNGGDSITGSDLSIINQGMILGGSGGSGA
                     DHNGDGGEAVTGDNLFIINGEIISGGHGGDSYSDSDGGNGGDAVTGVNLPIINKGTIS
                     GGNGGNNYGEGDGGNGGDAITGSSLSVINKGTFAGGNGGAAYGYGYDGYGGNAITGDN
                     LSVINNGAILGGNGGHWGDAINGSNMTIANSGYIISGKEDDGTQNVAGNAIHITGGNN
                     SLILHEGSVITGDVQVNNSSILKIINNDYTGTTPTIEGDLCAGDCTTVSLSGNKFTVS
                     GDVSFGENSSLNLAGISSLEASGNMSFGNNVKVEAIINNWAQKDYKLLSADKGITGFS
                     VSNISIINPLLTTGAIDYTKSYISDQNKLIYGLSWNDTDGDSHGEFNLKENAELTVST
                     ILADNLSHHNINSWDGKSLTKSGEGTLILAEKNTYSGFTNINAGILKMGTVEAMTRTA
                     GVIVNKGATLNFSGMNQTVNTLLNSGTVLINNINAPFLPDPVIVTGNMTLEKNGHVIL
                     NNSSSNVGQTYVQKGNWHGKGGILSLGAVLGNDNSKTDRLEIAGHASGITYVAVTNEG
                     GSGDKTLEGVQIISTDSSDKNAFIQKGRIVAGSYDYRLKQGTVSGLNTNKWYLTSQMD
                     NQESKQMSNQESTQMSSRRASSQLVSSLNLGEGSIHTWRPEAGSYIANLIAMNTMFSP
                     SLYDRHGSTIVDPTTGQLSETTMWIRTVGGHNEHNLADRQLKTTANRMVYQIGGDILK
                     TNFTDHDGLHVGIMGAYGYQDSKTHNKYTSYSSRGTVSGYTAGLYSSWFQDEKERTGL
                     YMDAWLQYSWFNNTVKGDGLTGEKYSSKGITGALEAGYIYPTIRWTAHNNIDNALYLN
                     PQVQITRHGVKANDYIEHNGTMVTSSGGNNIQAKLGLRTSLISQSCIDKETLRKFEPF
                     LEVNWKWSSKQYGVIMNGMSNHQIGNRNVIELKTGVGGRLADNLSIWGNVSQQLGNNS
                     YRDTQGILGVKYTF"
     gene            4416..5618
                     /gene="virA"
                     /locus_tag="OO357_003845"
     CDS             4416..5618
                     /gene="virA"
                     /locus_tag="OO357_003845"
                     /EC_number="3.4.22.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-tubulin-specific protease"
                     /protein_id="HCR5888616.1"
                     /translation="MQTSNITNHERNDSSWMSTVKSTTEVSWNKLSFCDILLKIITFG
                     IYSPHETLAEKHSEKKLMDSFSPSLSQDKMDGEFAHANIDGISIRLCLNKGICSVFYL
                     DGDKIQSTQLSSKEYNNLLSSLPPKQFNLGKVHTITAPVSGNFKTHKPAPEVIETAIN
                     CCTSIIPNDDYFHVKDTDFNSVWHDIYRDIRASDSNSTKIYFNNIEIPLKLIADLINE
                     LGINEFIDSKKELQMLSYNQVNKIINSNFPQQDLCFQTEKLLFTSLFQDPAFISALTS
                     AFWQSLHITSSSVEHIYAQIMSENIENRLNFMPEQRVINNCGHIIKINAVVPKNDTAI
                     SASGGRAYEVSSSILPSHITCNGVGINKIETSYLVHAGTLPSSEGLRNAIPPESRQVS
                     FAIISPDV"
     gene            5797..>5983
                     /locus_tag="OO357_003846"
     CDS             5797..>5983
                     /locus_tag="OO357_003846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5888617.1"
                     /translation="MTKNTRFSPEVRQRAVRMVLESQGEYDSQWAAICSIAPKIGCIL
                     ETLRVWVRQHERDTGGGE"
BASE COUNT         1840 a         1355 c         1018 g         1770 t
ORIGIN      
        1 tcaggctgta cgcttcccca tgtgatttgc ctcgccactt caccgggctc aggaccccgt
       61 aggatcttca ccgaaaaaaa tgcatcacca gttgctttat tatttgtttt aggtagacct
      121 gttactaatt tatacctact ctgaccaagt ctggataaga aaggcacacc tgcacatttc
      181 tacttggaaa tgttttcttt taccattttg ctcaaaaatt tcaacatcca atattcctcg
      241 tacagaacta ctcaagtcct gaatagaaaa agttccagct ggtacagtag agtcatatat
      301 tactcgacct tgttgagata caacaacacg tgcattagtt tctgcaatac cagtaatctg
      361 aggcgcataa ccacgaatat ttggcggtag cattcgttca tcgctttcta atgcaattcc
      421 tgtgtaacgc caagcaacag cgcattctgg cgcatgatct ggtaaacacg tttggcattg
      481 atcgcaggca taccatcatg tgcacaaaaa cccaccgagc ttttacacgg tgggtcccag
      541 agaaatgcag gacatcaaca cgccctgcat ttttattatc agaaggtata tttcacaccc
      601 aaaatacctt gggtgtctct gtaactgtta ttacccaatt gctgagatac gtttccccag
      661 atgcttaggt tatctgcaag acgccccccc acaccagttt tgagttcaat cacattacgg
      721 ttgcctatct ggtgatttga catgccattc ataattacac catattgctt tgagctccat
      781 ttccaattca cttccaaaaa tggttcgaac ttacgaagag tctccttatc gatacaactc
      841 tgactaatta aggatgtacg caatcccaat tttgcttgaa tattattgcc cccagaggat
      901 gtgaccatag tgccattgtg ttcaatatag tcgtttgctt ttaccccatg cctagttatc
      961 tggacttgtg gattgagata caatgcgttg tcaatattat tatgagcagt ccagcgtatg
     1021 gttgggtaga tatagccagc ttccaaagct cctgttattc ctttgctgga atatttctca
     1081 ccagttaacc catctccttt gactgtatta ttaaaccaac tgtactgcaa ccaagcatcc
     1141 atatatagac ctgttcgttc tttttcatcc tgaaaccaag aactgtacaa accggcagta
     1201 taaccgctca cagttcctcg tgaactataa ctagtatact tattatgagt tttgctatcc
     1261 tgatatccat aagctcccat aatacccaca tgcaagccat catgatcagt gaagtttgtc
     1321 ttcaaaatat ctccaccaat ctgataaacc atcctgttag ctgtggtttt taattgtcta
     1381 tcagctaaat tatgctcatt atgtccacca acagtacgaa tccacatggt ggtttcgctg
     1441 agctgacctg tagtaggatc aacaatagtg ctaccgtgtc ggtcatagag agaaggacta
     1501 aacatcgtgt tcattgctat caggttagca atataactgc cagcttcagg gcgccatgtg
     1561 tgaatactac cttcacccaa attaagtgaa gatacaagct gtgaactagc tcggcgacta
     1621 ctcatttgag tagactcttg attgctcatc tgttttgatt cttgattatc catctgacta
     1681 gttagatacc acttattggt attcagtcca gatacagtgc cctgtttcag gcgatagtca
     1741 taactaccag caacaatacg gcctttctga ataaaagcat tcttatcaga agaatctgtc
     1801 gaaataattt gaacaccttc aagagtttta tctccagagc ctccctcatt tgtcactgca
     1861 acataggtaa taccagacgc atggcctgca atttccagcc ggtcagtttt actgttgtca
     1921 ttgccgagaa ccgcgcccaa agataatatt ccgccctttc catgccaatt acctttctga
     1981 acataggtct gaccgacatt tgacgaacta ttattgagaa taacatgacc gtttttctcc
     2041 agagtcatgt tacctgtgac aatgacgggg tcaggcaaaa aaggggcatt aatattattg
     2101 attagcacag tcccactatt taataaagtg ttaacagttt ggttcatgcc tgaaaaattc
     2161 aaggtagcac ctttattaac aataacacca gcggtacgtg tcatagcttc aactgtcccc
     2221 atttttagaa tgcctgcatt gatgttggtg aaaccagagt aggtattttt ttccgccaaa
     2281 atgagagttc cctcccctga ttttgttagg gattttccgt cccagctatt tatattatga
     2341 tggctgagat tatctgccag aatagtacta acagtaagtt cagcgttttc tttcagattg
     2401 aactctccat gactgtcgcc atctgtatca ttccagctca aaccgtagat caatttattc
     2461 tggtcactga tatagctttt tgtatagtca atagcaccag tagtgagtaa cggattgatg
     2521 atagatatat tagaaacact gaaacctgtt atccctttat ctgcacttag cagtttatag
     2581 tccttctgcg cccagttatt tataatagcc tccactttta cattgttgcc aaatgacata
     2641 tttccagaag cttccagact actgattcca gctaaattta aagaactgtt ctcaccaaaa
     2701 gaaacgtcac ctgaaacagt gaatttgtta cctgatagtg aaacagttgt acaatcacca
     2761 gcacataaat caccttcaat agttggtgtg gtcccagtgt aatcattgtt gataattttc
     2821 agaatggatg aattgttaac ctgtacatca ccagtaatga cagaaccttc atggagtatt
     2881 aatgaattgt ttccaccagt gatgtggata gcattacctg ctacattttg tgttccatca
     2941 tcttctttac ctgaaattat atatccgcta ttagcaatgg tcatattgct accatttata
     3001 gcatcccccc aatgtccacc attaccgcct aaaatagctc cattgttgat tacagacagg
     3061 ttatctcctg tgatagcatt accaccgtag ccatcataac cataaccgta agcagcacct
     3121 ccgttgcctc cagcgaacgt gcccttattg attacagaga ggctgcttcc tgtgatggca
     3181 tcacctccat taccgccatc accctcacca taattgttac ctccattacc accggaaata
     3241 gtccctttgt tgattatggg taaattgact cctgtgacgg catcacctcc attcccccca
     3301 tcactatcac tataactatc gccaccatgt ccacctgaaa taatttctcc atttattata
     3361 aacagattgt ctcctgtaac agcctcacca ccatcaccgt tatggtcagc accgctaccg
     3421 ccgctaccac caagaatcat gccttgattg attatagaca agtcagatcc ggtaatagag
     3481 tcaccaccat taccgccaca actattacca tcattattat caccagcacc accatgacct
     3541 gcgctaatca taatatcaga gttaatccgt agcttagttc tagatgcatg agaggggttg
     3601 gaagaagtta tataccacgc atccattcca tcttcaccag ctcccaaggg agaaagtcca
     3661 tcaacaggtg ttaataattt ttcataattg tcctctgaaa aatgaagttc ttgagtaccc
     3721 gaaagaggag tagcaaaagc tattggcccc ccgagcaaca gggatgcacc aacaacaaaa
     3781 ggagaaaaag acaatttgca tgttttttcc tttaccgtag gtaattctcc ggcccccccc
     3841 tgtgaacatt gggtcatatt acaaaaaaat ttgtgaattt gattcatgca ctatattatc
     3901 agtaagtggt tgataaaccc ctgaaaagat tccgttttat ccggaataaa gggacgatat
     3961 atgcaaaaac atattaaaca aagcctcaac caaaacatga gagctgtgtt atatatcaat
     4021 gcaacgggag aaatttacct gtgtcttgct ttgaaaaaaa gagggggaaa gctatatgtt
     4081 cgataataaa ttaataattt tactatgata gtctgcttca gaccgcaact tttcagttct
     4141 ttctgtacgc ttgcccaaag tttaaccact ttgtttttct atacacccaa tggttctatc
     4201 tgccccttga tgttaatccc caaagccaat aacatgaatg tattcatatc acaactttcc
     4261 taccagactc tccaatgcaa taactgcata attgttttta gtggaaatgt atcgttttct
     4321 taaagagaag aataacattc cattttatta tgttcccaat ttaattatat taatatatat
     4381 tggcattaat aggaaaatac atcaggagaa atcaaatgca gacatcaaac ataactaacc
     4441 atgaaagaaa tgactcatca tggatgtcaa ctgtcaaatc aactacagaa gtaagttgga
     4501 ataaattgag tttttgtgac attttactga aaataataac tttcggcata tattctccac
     4561 acgaaactct agccgaaaaa catagcgaaa aaaaactaat ggatagtttc tcaccatcac
     4621 tatcacaaga taaaatggac ggagagtttg cccacgcaaa catagatggc atttcaatac
     4681 ggctatgcct taacaaaggc atatgttcgg ttttttatct tgatggagat aaaatacaat
     4741 ccactcaact ttcatcaaaa gaatataata acttgttaag ttcactcccc cccaaacaat
     4801 tcaacttagg aaaagtacat actattacag ctccggtatc agggaatttt aaaactcaca
     4861 agcctgcacc agaagttatt gaaacagcca ttaattgctg tacatcgata ataccaaacg
     4921 atgattactt ccacgttaaa gacactgatt ttaatagtgt ttggcatgat atttacagag
     4981 atattcgagc atctgacagc aatagtacaa aaatatattt caacaatatt gaaataccac
     5041 taaaattaat tgcagatcta attaatgagt taggaattaa tgaattcatt gatagcaaaa
     5101 aagagcttca aatgctaagt tacaaccaag taaataaaat aatcaattca aacttccccc
     5161 aacaagacct atgttttcaa acagaaaaac ttttgtttac ttcactcttt caagaccctg
     5221 catttatatc tgcactaaca tctgcattct ggcaatctct tcacatcacg tcttcctctg
     5281 tggaacacat atatgcccaa attatgtccg aaaacataga aaacaggctt aatttcatgc
     5341 ctgaacaacg agttattaac aattgtggac atattataaa aatcaacgca gttgtcccta
     5401 aaaacgacac tgcaatctct gcctctgggg ggagggctta cgaagttagc tcatcaatat
     5461 taccatcgca cataacatgt aatggtgttg gtattaataa aatagaaaca agctatctcg
     5521 ttcatgctgg aacacttcca agcagtgaag ggcttcgcaa tgctatccct ccagaaagtc
     5581 gtcaagtatc atttgccatc atatctcctg atgtttaagc caatattata ttggctgcca
     5641 gactgtaaaa aaacgttctt cagaacagct ctataaacta ttaaaccaaa gctaaaagga
     5701 tggggaccaa tagaatctca gcccatcctt tttatatcat tatgaaccgc cccggaaatc
     5761 ctggagacta aactccctga gaaagaggta aacaggatga ctaaaaatac acgtttttcc
     5821 cctgaagtcc gtcaacgggc agttcgtatg gttctggaaa gtcagggcga atatgactca
     5881 caatgggcag cgatttgttc cattgcccca aagattggct gtatactgga aactctgcgt
     5941 gtctgggtac gccagcatga gcgggatacc gggggcggtg agg
//
LOCUS       DAJTRC010000170         5718 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.171, whole genome shotgun sequence.
ACCESSION   DAJTRC010000170 DAJTRC010000000
VERSION     DAJTRC010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5718)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5718)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5718
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.171"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..231
                     /locus_tag="OO357_003847"
     CDS             <1..231
                     /locus_tag="OO357_003847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888618.1"
                     /translation="FYLVTRVCGFANGYFRVRTSEPVRGNVFLHNMTAFNKLAPRTVG
                     KREHKRGMYVYYCIIFCHRVILNGPARGPCGC"
     gene            complement(228..1169)
                     /locus_tag="OO357_003848"
     CDS             complement(228..1169)
                     /locus_tag="OO357_003848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2184 domain-containing protein"
                     /protein_id="HCR5888619.1"
                     /translation="MITFDQATVDSSGAFLIGELERLDQTLNLPLVGYTWSRDIQLRE
                     DASIADDISSWTNTSFAAAGTGANPNGKNWVGKDSTAIAGVNVDIGKSGNPLNLWGME
                     LGWTVIELQAAQQVGRPIDTQKYDGMQLKWQMDNDEQVYVGDSALNLKGLVTLDGVPV
                     NNAAKTWATSTPDEIRASINQVLSDAWAASGYSVVPRDLLIPPEQFALLSSIIVSSAG
                     NQSLLTNLQTNTISYHQNGVPLNIRAVKWLKGRGVGNKDRIVAYTNDKKYVRYPLVPL
                     QSVPVQYRGLYQIVTYYGKLGAVEPVYKETISYVDGI"
     gene            complement(1181..1670)
                     /locus_tag="OO357_003849"
                     /pseudo
     CDS             complement(1181..1670)
                     /locus_tag="OO357_003849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001066728.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1674..2894)
                     /locus_tag="OO357_003850"
     CDS             complement(1674..2894)
                     /locus_tag="OO357_003850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2213 domain-containing protein"
                     /protein_id="HCR5888620.1"
                     /translation="MKYFFETRLGETRYRLADGSLLCKDVPIGRTGKQLYGADDLPKL
                     KPDKFVEIVVTRSPEQVFHPATLASFEGMSITILHPEDENGNVRLVNPENWKVLAVGH
                     LQNVRRGTGEQSDLMLADLIVKDESAIQLIEDGLREVSCGYDAEYEQTEPGKAEQVDI
                     TGNHVALVPKGRAGNRCAIGDRDTMANQKKNWWNRMRAAIKTGDADTMNELVESAPAS
                     VTGDEGDLPQGVNLNINLSPQQPLPDKAPEMGGDPTGDSDDDLKTLLKALLAKLERNA
                     TGDNDNKPDDNPTGDGEDDEEETTITGDSAWRAEVIVPGIDLSRKMKPTAFKREVLAS
                     ADKTLVRQIVGDADIRKLPKQSVDMAFNAVSEVAKGRNTRATTGDAQRLNMGMTSIAS
                     LNKQNAEFWANRKG"
     gene            complement(2898..3641)
                     /locus_tag="OO357_003851"
     CDS             complement(2898..3641)
                     /locus_tag="OO357_003851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706597.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HCR5888621.1"
                     /translation="MTNRRSRQAEIRYRTSLRRIARAVGDIVNGRYDGSNDSITEIIA
                     ALERYSEIITPWATKVAENFTADLTRQNEKVWRQHSKNISRELRNLVESAPVGQVMQS
                     IIAEQVKYIKSLPLEAADRVYDIQNKAIEAVVTGGRAEQFAKEIASTGDVAKSRADLI
                     ARTELGRATGALDMARAIAIGSDGYIWRTADDGDVRDSHDHMKGKFVRWDSPPTLDGM
                     TGHAGELPNCRCYKEIVFVRVPFAMKRAA"
     gene            complement(3532..4998)
                     /locus_tag="OO357_003852"
     CDS             complement(3532..4998)
                     /locus_tag="OO357_003852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1073 domain-containing protein"
                     /protein_id="HCR5888622.1"
                     /translation="MARNKQALRRTVQATADGYENFIARVGMQTPNQHSASTYRANFT
                     SRNRMLVEWSYRSSWIIGEAVDAIPDDMTRKGIRITSEIDAKDRGILESQLDELQIWD
                     ALNDVLKWSRLYGGAVGFIMIEGQAPMTPLRPETIGKGKFKGILPLDRWMVDPALTRR
                     IKDMGPDLGKPEFYDVVTTATGIPAWRIHHSRLIRFDGVTLPFQQKMTENEWGMSVVE
                     RIWDRLTAFDSATVGAAQLVYKAHLRTYSVEKLRELIALGGPAYEALLKNIDLIRQFQ
                     SNEGMTLMDSRDKFETHQYSFSGLDDILSQFAEQISGAVGIPLVRLFGQSPKGFSTGD
                     ADLANYYDRISSLQERRLRLPVRRILDIMHRSELGKPLPDDFTFEFNPLWQMSDVDRS
                     TVALNTTNAISTAMGDGLMTLKAAMTDLRENSDVTGIGASITNEDIENAEDEAPPGIG
                     EPDDEPQEPSGGNPVSNQPTQDSEGGRRHRKWSLRWFK"
     gene            complement(4998..5231)
                     /locus_tag="OO357_003853"
     CDS             complement(4998..5231)
                     /locus_tag="OO357_003853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005125539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HCR5888623.1"
                     /translation="MDELISIDSRCPLLEKLKLELTTPHRDFDRNGRVMVESKKDLAK
                     REIPSPNVADAFIMAFAPIDTSLDIWEQLGRQA"
     gene            complement(5219..5698)
                     /locus_tag="OO357_003854"
     CDS             complement(5219..5698)
                     /locus_tag="OO357_003854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005049509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888624.1"
                     /translation="MDAHKTLNFEPSGRKRIGFDVADSGTDKCANVYRHGSVVFWADE
                     WKAKEDELLKSCQRTYQAALEREADIVYDSIGVGASAGAKFSEINADRKSENAYARRV
                     NYQRFNAGAGVHEPDDEYNGIPNKDFFANLKAQAWWLTVSEIRLTPLTTENSILWMS"
BASE COUNT         1223 a         1616 c         1429 g         1450 t
ORIGIN      
        1 ttttacctcg ttacgcgcgt ctgtggcttt gccaacggat acttccgcgt gcgcacgagt
       61 gaaccagtgc gtggcaacgt cttcctccac aacatgacgg cctttaacaa actcgcgccg
      121 agaaccgtcg ggaagcgtga gcacaaacgg ggtatgtacg tgtattactg catcattttt
      181 tgccatcggg tcatccttaa tggccccgcc agggggccat gtggctgtta aatgccatca
      241 acgtacgaaa tggtttcttt gtacactggc tcgactgcac ccagcttgcc gtagtaagtg
      301 acgatctgat acagaccgcg atactgcacc ggcacgctct gaagcggaac cagcgggtag
      361 cggacgtatt ttttatcgtt ggtgtacgca actatgcgat ccttattccc cacaccacgg
      421 cctttcagcc atttaaccgc gcggatattc agcggaacac cgttctggtg atagctgatg
      481 gtgttggtct gaaggttcgt caacagggac tggttacctg cagatgaaac gatgatgctg
      541 gacaacagag caaactgctc aggcgggatc agcaaatcac gcggaaccac agagtaacca
      601 gaagcggccc acgcatcaga cagcacctgg ttaatgcttg cgcggatttc gtccggtgtt
      661 gaggttgccc acgttttggc agcgttgttg acaggcacgc cgtccagggt aacaaggcct
      721 ttcaggttta atgcggaatc gccaacatat acctgttcat cgttatccat ctgccatttc
      781 agttgcatac cgtcatactt ctgcgtatca atcgggcggc cgacctgctg agcagcctgc
      841 aattctatga ccgtccagcc aagttccatc ccccacaggt tcagcgggtt accggatttg
      901 ccgatatcca cgttcacgcc agcaatagcg gttgagtctt tgcctaccca gttttttcca
      961 ttcggatttg cgccagtacc cgcagcggcg aagctggtat tcgtccagct ggaaatgtca
     1021 tctgcgatgg aggcgtcttc acgcagttga atatcgcggc tccaggtgta ccccaccagt
     1081 ggcaggttca gcgtctggtc gagtctctcc agctccccga tgagaaaggc accagagctg
     1141 tcaacggttg cctgatcaaa agtaatcatt cgtctgttcc ttaaatcttc caggaaattt
     1201 ctgcattgcc gtcagcatca ccggcacctg tgaattcagc gttggtcagc accacgtttt
     1261 tgccactgac tgacgtggac atgaatccac ccagaggcac tttgatggat tcatcagtgg
     1321 agatcgcctt ttttgatagt gctggcatca aaatcagaac cgagattaac ggtcacgtag
     1381 ccacgcttca tggcgtcgcc cgggaagttc ttgcctgttc ccacctggcg aaccatgtcc
     1441 ggctgcgacg tggtcggata agggcgcacg tagatcccct tcaccttgtc tgcggtatca
     1501 ccatctgcca gcggcacgaa aaaaccgtca tcatcgtatt taccagccag gccataggca
     1561 gcgaaggcgt tatcggattt aaggaccacc ggttcgacgg ttaagtcctg cgggcgagag
     1621 acagccccgg caatgccaac aggcatccgg tacagaaata cattattcat tttttaccct
     1681 ttacggtttg cccagaattc agcgttttgt ttgttcaggg aagcgatact ggtcatgccc
     1741 atgtttaggc gctgtgcatc gccggtggtg gcgcgggtgt ttcgcccttt ggcaacctca
     1801 gacacggcat taaacgccat gtcgaccgat tgtttcggta atttgcggat atccgcatca
     1861 ccgactatct ggcgaaccag cgttttgtca gcagaagcca gaacctcgcg tttgaacgcg
     1921 gtcggtttca tcttacggct cagatcgata cccggaacga taacttcggc acgccaggct
     1981 gagtcaccag taatcgtggt ttcctcttca tcgtcctcgc cgtcaccggt cggattatcg
     2041 tcaggcttat tatcgttatc gcccgtggca tttctttcca gcttagccag cagggctttc
     2101 agtaatgttt tgaggtcatc atcactgtcg ccggttggat ctccgcccat ctctggtgct
     2161 ttgtccggta gtggttgctg cggggacagg ttgatgttga gattaacgcc ctgcggcaaa
     2221 tccccctcat ctcctgtaac cgatgcggga gccgactcca ccagttcgtt catggtgtcg
     2281 gcatctcctg tcttgatggc cgcacgcatg cggttccacc agtttttctt ttgatttgcc
     2341 attgtgtctc tgtctccaat tgcacaacga tttccggctc tgcctttagg gacaagagcc
     2401 acatggtttc cggtaatatc gacctgctcg gctttacctg gctcggtctg ctcgtactcc
     2461 gcgtcatagc cgcacgacac ttcgcgcagg ccatcttcga taagctgaat ggcgctttcg
     2521 tctttgacga taaggtcagc cagcatcaaa tcagactgct cacccgtccc gcgccggaca
     2581 ttctggaggt gcccgacagc aagcactttc cagttctcgg gatttaccag ccgcacattc
     2641 ccgttttcat cttcaggatg caggatcgtg atgctcatcc cttcgaatga ggcaagcgtg
     2701 gccggatgga atacctgctc aggagaacgc gtgacgacta tttcaacgaa cttatcgggt
     2761 ttcagttttg gcaggtcatc agcaccatag agctgcttac ctgttcgtcc tatcggcacg
     2821 tctttgcaca gcaacgagcc gtcagccagg cggtatcggg tttccccgag cctggtctca
     2881 aaaaagtatt tcatgggtta tgctgccctt ttcattgcga atggaacgcg aacaaacacg
     2941 atctctttat agcagcggca gtttggcagc tcgcccgcgt ggccggtcat gccgtccaaa
     3001 gttggaggtg aatcccagcg gacgaattta cctttcatgt gatcgtggga atctcggaca
     3061 tcgccatcat cggctgtacg ccaaatataa ccatccgagc caatagctat cgctcgggcc
     3121 atatccagcg cgcccgttgc tcttcccagt tccgttcggg caatcagatc ggccctagac
     3181 ttagcaacat cgccggtaga tgcaatctct ttagcaaact gctccgcccg accaccggtg
     3241 acaacagcct ctatcgcttt gttctggatg tcgtacaccc tgtctgcggc ctcgagaggc
     3301 agagatttga tgtacttgac ctgttcggcg atgatggatt gcatcacctg gcccacagga
     3361 gcgctttcca caagattgcg gagctcgcga ctgatgttct tgctgtgttg ccgccaaact
     3421 ttctcgttct gccgggttag gtccgcagta aagttttccg cgacctttgt cgcccagggg
     3481 gtgatgattt cactgtaacg ctccagcgcc gcaataattt ccgtgatact gtcatttgaa
     3541 ccatcgtagc gaccatttac gatgtctccg accgccctcg ctatcctgcg taggctggtt
     3601 cgataccgga tttccgcctg acggctcctg cggttcgtca tcaggttcgc cgatgccggg
     3661 cggcgcttca tcttcggcat tctcgatgtc ctcgttggta atggatgccc ctatgccggt
     3721 tacgtcagaa ttttctcgca aatcagtcat agcggctttc agtgtcatca gaccatcacc
     3781 catcgccgta ctgattgcgt tggtggtgtt taacgccacc gttgagcgat cgacatcaga
     3841 catttgccag agcgggttaa actcaaacgt gaaatcgtcc ggcagcggct tgccaagttc
     3901 cgaacgatgc atgatgtcca gtatccgccg caccggaaga cgtaaacgcc tctcctgcaa
     3961 cgaactgatg cgatcgtaat agttggcaag gtctgcatca ccggtagaaa atcctttcgg
     4021 ggactgtccg aacaaccgca ccagtgggat accaacagcg ccactaatct gttctgcaaa
     4081 ctgcgaaagg atgtcatcca gaccactgaa gctgtactga tgggtttcaa acttatcccg
     4141 cgagtccatg agcgtcatgc cttcattact ctggaactgt cgaatcaggt cgatattctt
     4201 cagcaacgct tcatacgcag gaccaccaag tgcgataagc tcgcgtagct tctccacgct
     4261 gtaggtacgc aaatgcgcct tgtagaccag ctgcgccgcg ccgacagtag cgctgtcgaa
     4321 cgcggtaaga cgatcccaga tacgctctac aaccgacatt ccccattcgt tctcggtcat
     4381 cttctgctga aatggcaacg tgacgccatc aaagcgaatc aggcgactgt gatgaatgcg
     4441 ccaggcagga attcccgttg ctgtggtcac cacatcgtaa aactcaggtt tacccaggtc
     4501 cggccccata tctttaatgc ggcgggtcag tgccgggtcg accatccagc ggtcgagcgg
     4561 gagaatcccc ttaaacttgc ccttaccgat ggtttcgggt cgcagcgggg tcattggtgc
     4621 ctgcccctca atcatgatga aacccaccgc gccgccgtag aggcgcgacc atttcagcac
     4681 gtcattcagc gcatcccaga tttgcaactc atccagttgt gattcgagaa tgccacgatc
     4741 ttttgcatca atttccgaag tgatgcgaat gcctttgcgg gtcatatcat ccgggatagc
     4801 atcgactgct tcgccgatga tccaggatga acgataggac cattccacca gcatgcggtt
     4861 acgactggtg aaattagccc ggtaggtgga tgctgagtgc tggttaggtg tctgcatccc
     4921 tactcgggca ataaaattct cataaccatc agctgtggcc tgcacagttc gccgcagggc
     4981 ttgtttgttt cgtgccatca ggcctgtctc cccagctgtt cccagatatc cagcgatgta
     5041 tcgattggcg cgaaggccat aatgaatgca tcagcaacgt ttggtgacgg tatctcgcgt
     5101 tttgcgaggt cttttttact ttcgaccatc acacgtccgt tacggtcgaa atcacgatga
     5161 ggtgttgtca gttccagttt cagcttttca agcaacggac aacgagaatc tatgctgatc
     5221 agctcatcca caggatactg ttctccgttg ttaatggcgt taaacgtatt tctgaaacgg
     5281 tcagccacca tgcctgagcc ttaagatttg cgaaaaagtc tttgttgggg atgccgttgt
     5341 actcgtcatc tggctcatgc acaccagcac cggcgttaaa cctctggtaa ttcacacgtc
     5401 gcgcgtatgc gttctcgctc ttccggtcag cgttaatttc agagaattta gcaccggcag
     5461 acgcaccaac accgatagag tcgtaaacaa tatctgcttc acgctccagc gccgcctgat
     5521 aagtacgctg gcagctcttc agtaattcat cttctttggc cttccattcg tcggcccaga
     5581 aaacaacgga tccgtgacgg taaacgttag cgcacttatc tgtaccactg tcagccacgt
     5641 caaagccaat acgctttctt ccactgggtt cgaaatttaa cgttttgtgc gcatccactg
     5701 cggcttctat ccaagaca
//
LOCUS       DAJTRC010000171         5601 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.173, whole genome shotgun sequence.
ACCESSION   DAJTRC010000171 DAJTRC010000000
VERSION     DAJTRC010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5601)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5601)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5601
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.173"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..411
                     /locus_tag="OO357_003855"
     CDS             <1..411
                     /locus_tag="OO357_003855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000080164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5888625.1"
                     /translation="AVRQQRSKALLTSLNEWMVEKNGTLSKKSRLGEAFSYVLNQWDA
                     LCYYSDDGLAEADNNTAERALRTVCLGKKNYMFFGSDHGGDRGALLYGLIGSCRLNGI
                     DPEAYLRHILSVLPEWPSNRVDELLPWNVVLTDK"
     gene            443..1057
                     /gene="istB"
                     /locus_tag="OO357_003856"
                     /pseudo
     CDS             443..1057
                     /gene="istB"
                     /locus_tag="OO357_003856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001522280.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element ISEc10 family helper ATPase
                     IstB"
     gene            complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="OO357_003857"
     CDS             complement(1226..5320)
                     /gene="sepA"
                     /locus_tag="OO357_003857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin SepA"
                     /protein_id="HCR5888626.1"
                     /translation="MNKIYYLKYCHITKSLIAVSELARRVTCKSHRRLSRRVILTSVA
                     ALSLSSAWPALSATVSAEIPYQIFRDFAENKGQFTPGTTNISIYDKQGNLVGKLDKAP
                     MADFSSATITTGSLPPGDHTLYSPQYVVTAKHVSGSDTMSFGYAKNTYTAVGTNNNSG
                     LDIKTRRLSKLVTEVAPAEVSDIGAVSGAYQAGGRFTEFYRLGGGMQYVKDKNGNRTQ
                     VYTNGGFLVGGTVSALNSYNNGQMITAQTGDIFNPANGPLANYLNMGDSGSPLFAYDS
                     LQKKWVLIGVLSSGTNYGNNWVVTTQDFLGQQPQNDFDKTIAYTSGEGVLQWKYDAAN
                     GTGTLTQGNTTWDMHGKKGNDLNAGKNLLFTGNNGEVVLQNSVNQGAGYLQFAGDYRV
                     SALNGQTWMGGGIITDKGTHVLWQVNGVAGDNLHKTGEGTLTVNGTGVNAGGLKVGDG
                     TVILNQQADADGKVQAFSSVGIASGRPTVVLSDSQQVNPDNISWGYRGGRLELNGNNL
                     TFTRLQAADYGAIITNNSEKKSTVTLDLQTLKASDINVPVNTVSIFGGRGAPGDLYYD
                     SSTKQYFILKASSYSPFFSDLNNSSVWQNVGKDRNKAIDTVKQQKIEASSQPYMYHGQ
                     LNGNMDVNIPQLSGKDVLALDGSVNLPEGSITKKSGTLIFQGHPVIHAGTTTSSSQSD
                     WETRQFTLEKLKLDAATFHLSRNGKMQGDINATNGSTVILGSSRVFTDRSDGTGNAVS
                     SVEGSATATTVGDQSDYSGNVTLENKSSLQIMERFTGGIEAYDSTVSVTSQNAVFDRV
                     GSFVNSSLTLGKGAKLTAQSGIFSTGAVDVKENASLTLTGMPSAQKQGYYSPVISTTE
                     GINLEDNASFSVKNMGYLSSDIHAGTTAATINLGDSDADAGKTDSPLFSSLMKGYNAV
                     LRGSITGAQSTVNMINALWYSDGKSEAGALKAKGSRIELGDGKHFATLQVKELSADNT
                     TFLMHTNNSRADQLNVTDKLSGSNNSVLVDFLNKPASEMSVTLITAPKGSDEKTFTAG
                     TQQIGFSNVTPVISTEKTDDATKWVLTGYQTTADAGASKAAKDFMASGYKSFLTEVNN
                     LNKRMGDLRDTQGDAGVWARIMNGTGSADGDYSDNYTHVQIGVDRKHELDGVDLFTGA
                     LLTYTDSNASSHAFSGKNKSVGGGLYASALFNSGAYFDLIGKYLHHDNQHTANFASLG
                     TKDYSSHSWYAGAEVGYRYHLTKESWVEPQIELVYGSVSGKAFSWEDRGMALSMKDKD
                     YNPLIGRTGVDVGRAFSGDDWKITARAGLGYQFDLLANGETVLQDASGEKRFEGEKDS
                     RMLMTVGMNAEIKDNMRLGLELEKSAFGKYNVDNAINANFRYVF"
BASE COUNT         1378 a         1402 c         1333 g         1488 t
ORIGIN      
        1 gccgtcaggc agcagcgaag caaagcgtta ctgacgtcgc tgaatgaatg gatggtggag
       61 aagaatggta cgctgtcgaa aaaatccaga ctgggcgaag cgttcagcta tgtactgaat
      121 cagtgggatg ccctctgtta ttacagtgat gacggtctgg cggaggcgga caataacaca
      181 gcagaaagag cgcttcgtac cgtctgtctc gggaaaaaga attacatgtt cttcggcagc
      241 gatcatggag gcgatcgcgg agctctgctg tacggactaa tcgggagctg tcggttgaac
      301 ggtatcgatc cagaagcgta tctgcgccat atcctgagcg tactgccgga atggccttcc
      361 aaccgagttg acgaactgct gccatggaac gtagttctca ccgataaata agcgtcaata
      421 cggccttccg ttgacgctta cagaactcct gtggcgcgag agtgaaaaac tgcgtcgtta
      481 taaaaaggag gcccggttgc cagttgccaa aacgctaagc gaatacgact tcattcagtt
      541 gccggaactg aatggagctc agttccagca actctgtgaa acgacagact gggttgatgc
      601 tggagaaaac gttctgctgt tcggggccag cgggttgggg aaaagccatc tggcggcagc
      661 gatcgtggat ggcgtagtag gccagggcta ccgggcccgg ttctacagcg caggagagct
      721 gttgcaggaa ctacgtaaag ccagagctca gttgaaactg aatgagctgc tactgaaact
      781 ggatcgctac cgggtgatag tggtggatga tcttggctat gtcaaacgcg acagcgccga
      841 aacgggtgtg ctgttcgagt taatagcgca tcgctatgaa cgtgggagcc tggtgataac
      901 cagtaaccat ccgttcagca tgtggggcag catcttcgtg gatgagacta tggcggtggc
      961 ggcagcagac cgactgatcc atcacggata tatgttcgaa ctgaaagggg aaagctacag
     1021 gaaaaagaca gcgaaggcag taacaagcgt gacttgatgt cgcactgaag gggcggccag
     1081 tatagttggc gccagtcggc aaaactagtt gacgtctaat acctggacgg tcacttctga
     1141 gaaaaggcat ttacacagaa tcttaaacag gctcacggag ttctttcaga aaaaggcctg
     1201 ccgaatggca gacctatccc attcgtcaga aaacataacg gaagttggcg tttatcgcat
     1261 tatccacatt atatttcccg aacgctgatt tctccagctc cagtcccaaa cgcatattat
     1321 ccttaatttc cgcattcatc cctaccgtca tcagcatcct gctatctttt tcaccttcga
     1381 aacgtttctc tccggaagca tcctgcagaa ccgtttctcc gttcgccagc aggtcgaact
     1441 gataacccag cccggctcga gctgtgattt tccagtcgtc tccggagaag gctcttccca
     1501 cgtcaacacc agtacggcca atcagtgggt tataatcctt gtctttcatg ctcagagcca
     1561 ttccccggtc ttcccagcta aaagcttttc ctgatacaga accgtaaacc agctctatct
     1621 gtggctccac ccaggactct ttcgtcaggt ggtaacgata accaacttca gcaccggcat
     1681 accaggaatg agagctgtag tcttttgttc ccagtgaggc aaaattcgcc gtgtgctgat
     1741 tatcatgatg gagatattta ccaatcaggt caaaataagc tccggaatta aagagtgcag
     1801 aggcatacag accgccaccc acggatttgt tttttccact gaatgcgtgg ctgcttgcat
     1861 tgctgtccgt ataggtcagc aatgcccccg taaataaatc cacaccgtcc agctcatgct
     1921 ttctgtcgac accaatctga acgtgagtgt agttatcgct gtagtcacca tctgccgaac
     1981 cggtaccatt cattatgcgt gcccagacac cggcatcccc ctgagtatcc cgcaggtcac
     2041 ccatacgttt gttcaggtta ttgacctctg taaggaagga cttataacct gatgccataa
     2101 agtcttttgc ggctttcgag gctccggcat cagcggtagt ctgatatcct gtcagcaccc
     2161 attttgtggc atcatccgtt ttttccgtgc tgattactgg cgtaacgtta ctgaaaccaa
     2221 tctgctgcgt accggcagtg aacgttttct catcactccc tttcggtgcg gtaattaacg
     2281 tcacgctcat ttcactggct ggtttgttta aaaagtcaac caggacgcta ttattactgc
     2341 ctgacagttt gtctgtgaca ttcaactggt cagcccggct gttgttggta tgcatcagga
     2401 acgtggtatt atctgcagaa agctctttta cttgtaaagt ggcaaagtgt ttcccgtccc
     2461 ccagttcaat tcgcgagccc ttagccttca gtgcaccggc ctctgatttt ccgtcagagt
     2521 accacagagc attgatcata tttaccgtac tctgtgcccc cgtaatactg cctctcagaa
     2581 cagcgttata tcccttcatt aaggagctga ataacggaga gtccgtcttc cctgcatcag
     2641 catcactgtc tcccagatta atggttgccg cagtggttcc tgcatgaata tccgaactca
     2701 gatagcccat atttttaaca gaaaagctgg cgttatcttc gaggttaatc ccttctgttg
     2761 tagaaattac gggtgagtaa tacccctgtt tctgcgcaga aggcatcccc gtcagggtca
     2821 gagaagcatt ttcttttaca tccacagccc cggtgctgaa aataccgctc tgagccgtaa
     2881 gttttgctcc ttttccgagg gtcaggctgc tgttgacaaa gctgccaaca cggtcaaaaa
     2941 cagcattctg agaggtcaca ctcacggtgc tatcataagc ctcaatacca ccggtgaatc
     3001 tctccatgat ttgcagggat gatttatttt ccagcgtaac atttccgctg taatcactct
     3061 ggtcaccaac tgtggttgca gtggcactcc cttcaacaga ggagaccgca ttaccggttc
     3121 cgtcactcct gtcagtaaag acacggctac ttcccagaat gactgtactt ccattggtgg
     3181 cgttaatatc tccctgcatc ttgccgtttc tggacagatg gaatgttgcc gcatcgagtt
     3241 tcagtttttc cagcgtaaac tgacgggttt cccagtcgct ctggctggaa gaagtcgtcg
     3301 ttccagcatg aataactgga tgaccctgga atatcagcgt gccggacttc ttggttatcg
     3361 aaccttctgg caggttaact gaaccatcaa gagccagtac atccttacct gagagctgcg
     3421 gaatattcac atccatattg ccgtttagtt ggccgtgata catataaggc tggctgctgg
     3481 cctcaatttt ttgttgcttc acagtatcaa tggctttatt acgatctttt ccgacattct
     3541 gccagacact gctgttgttc agatcggaga agaacgggga atatgaactc gctttcagga
     3601 tgaaatattg cttggtagaa ctgtcataat ataggtctcc cggcgctcct ctccccccaa
     3661 aaatactgac agtattaacc ggtacattga tgtcactggc ttttaatgtc tgaaggtcga
     3721 gtgttaccgt tgattttttc tcactgttat tggtaatgat ggccccgtag tcagccgcct
     3781 gaagacgggt aaaggtcaga ttattaccat tcagttccag acgtcctcca cggtatcccc
     3841 acgaaatatt atccggatta acttgctgtg agtcagaaag cacaactgtc ggacgaccgc
     3901 tggcaatacc aacagagctg aaagcctgta cctttccgtc agcatccgcc tgctggttga
     3961 gaataacggt accgtccccc accttgagtc cgcctgcatt cacaccggtc ccgtttactg
     4021 tcagggtacc ttcgccagtt ttatgcaggt tatcaccggc aactccgttc acctgccaca
     4081 gaacgtgggt tcctttatcg gtgataatcc cgccccccat ccatgtctgg ccgttcaggg
     4141 cggacaccct gtaatcgccg gcaaactgca gatacccagc tccctgatta acggaattct
     4201 gcagaacgac ctcgccatta ttgccggtga acaggaggtt ttttcctgcg ttcagatcat
     4261 ttcctttctt tccatgcata tcccaagtcg tgtttccctg agtcagtgtg ccggtgccat
     4321 tagccgcatc atatttccac tgcagtactc cttcaccaga ggtgtaggct atagttttgt
     4381 caaaatcatt ttgcggttgc tgaccaagga aatcctgagt ggtgacgacc cagttatttc
     4441 catagttagt tcctgaggaa agcacgccaa tcagtaccca ttttttttgc agggaatcat
     4501 aagcaaacag gggggagccg ctgtccccca tattcagata attagccaga ggtccattcg
     4561 cgggattaaa aatgtcaccg gtctgagctg taatcatctg tccgttgtta taggagttca
     4621 gcgcgctgac ggttccccca acaagaaatc cgccattggt atacacttgg gtgcgattac
     4681 catttttgtc tttgacgtac tgcatccccc ccccaaggcg atagaattca gtaaagcgtc
     4741 cgccggcctg ataggcaccg ctcacagccc cgatgtcaga cacttctgcc ggcgccacct
     4801 ctgtcaccag tttactcagg cggcgggttt taatatcaag accgctatta ttatttgtac
     4861 caaccgcagt ataggtgttt ttggcgtatc cgaagctcat cgtgtcagaa ccgctgacat
     4921 gtttggcggt aaccacatac tgaggggagt aaagtgtatg gtctcccgga ggcaggctac
     4981 cggtagtaat agtggcacta ctgaaatcag ccatcggggc tttatcaagt ttaccgacca
     5041 gattgccctg cttgtcgtaa atggaaatat tcgtggtccc cggcgtaaac tgacctttat
     5101 tctcggcaaa gtcgcggaat atctgataag ggatctctgc actgaccgtt gctgacagag
     5161 ctggccatgc cgaggaaagt gataaagctg caacagacgt aagaataacc cgacgtgaaa
     5221 gtctgcgatg gcttttgcag gtaaccctgc gggccagttc ggagacagca atcaggcttt
     5281 tggttatatg gcaatactta agataatata ttttgttcat atagaaacat caacctaatt
     5341 ttgacaaaga ttaattacat aggatgtaga tggagcagtc cgagattcta ctaatatcaa
     5401 ttattaagca tctgtttttt agttggtgta atataatttt tattaaggta agcggaaaaa
     5461 caaccaaaaa aaacgcaaga tgttattttt ttatcatttt gagccagttt ggctgggggg
     5521 atactcttct cttaagcttc ttgctcaact ttaaaaatag ttatgccagt tcggtatcga
     5581 taaggggcag tttggatatc g
//
LOCUS       DAJTRC010000172         5491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.174, whole genome shotgun sequence.
ACCESSION   DAJTRC010000172 DAJTRC010000000
VERSION     DAJTRC010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.174"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(45..578)
                     /locus_tag="OO357_003858"
     CDS             complement(45..578)
                     /locus_tag="OO357_003858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706619.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme"
                     /protein_id="HCR5888627.1"
                     /translation="MSAKIKYGLSAAVLALIAAGASAPQILDQFLNEKEGNHTTAYRD
                     GSGIWTICRGATMVDGKPVIPGMKLSKEKCDQVNAIERDKALAWVERNIKVPLTEPQK
                     AGIASFCPYNIGPGKCFPSTFYKRLNAGDRKGACEAIRWWIKDVGRDCRIRSNNCYGQ
                     VIRRDQESALACWGIDQ"
     gene            complement(644..1273)
                     /locus_tag="OO357_003859"
     CDS             complement(644..1273)
                     /locus_tag="OO357_003859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888628.1"
                     /translation="MKHEEMNQRFNHLENEITELNKKLSALVSSEDENKRRDEHYAAI
                     YDYCHKVAHETFMKFLQEKFLPAALSEKEAAYLRPEYVITVNSAGEEEHKSDFIASAP
                     DKDQEPHRPFRVSCEEGEFVVYENGKPVRASHHHCLKIINLAIRCLKDENTRVMKRIG
                     RCMGYLQVAAEIEALASGADMDAAVREALLRDFNTPPLRKSLMTGSSRG"
     gene            complement(1277..1492)
                     /locus_tag="OO357_003860"
     CDS             complement(1277..1492)
                     /locus_tag="OO357_003860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II holin family protein"
                     /protein_id="HCR5888629.1"
                     /translation="MKSMDKISTGIAYGTSAGSAGYWFLQWLDQVRPSQWAAIGVLGS
                     LLLGLLTYLTNLYFKIREDKRKAARGE"
     gene            complement(1785..1859)
                     /locus_tag="OO357_003861"
     tRNA            complement(1785..1859)
                     /locus_tag="OO357_003861"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1824..1826),aa:Gly,seq:tcc)
     gene            complement(1863..1938)
                     /locus_tag="OO357_003862"
     tRNA            complement(1863..1938)
                     /locus_tag="OO357_003862"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1903..1905),aa:Thr,seq:tgt)
     gene            complement(1941..2017)
                     /locus_tag="OO357_003863"
     tRNA            complement(1941..2017)
                     /locus_tag="OO357_003863"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1981..1983),aa:Arg,seq:tct)
     gene            complement(2019..2095)
                     /locus_tag="OO357_003864"
     tRNA            complement(2019..2095)
                     /locus_tag="OO357_003864"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2059..2061),aa:Ile,seq:cat)
     gene            complement(2295..2675)
                     /locus_tag="OO357_003865"
     CDS             complement(2295..2675)
                     /locus_tag="OO357_003865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5888630.1"
                     /translation="MRDIQQVLERWGAWVANNHEDVTWSSIAAGFKGLIPSKVKSRPQ
                     CCDDDAMVICGCMARLNRNNNDLHDLLVDYYVFGMTFMTLARKHGCSDGYIGKKLQKA
                     EGVVEGMLMMLGVRLEMDKYIEHP"
     gene            complement(2668..2868)
                     /locus_tag="OO357_003866"
     CDS             complement(2668..2868)
                     /locus_tag="OO357_003866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000111769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ninH"
                     /protein_id="HCR5888631.1"
                     /translation="MSVKIQTIPELLIQTRGNMTEVSRMLNCNRATVRKYAEDKEGKG
                     HAIVDGVLIVHRGWDRGKDSDA"
     gene            complement(2963..3220)
                     /locus_tag="OO357_003867"
     CDS             complement(2963..3220)
                     /locus_tag="OO357_003867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888632.1"
                     /translation="MNNQTMTFTPEQLRKHAQEMLRHAEQLEKTGITKDAIRKDMVPA
                     LRELMQAKHRAQKAVDELVDCVAELETRVGKFEKLVQEALR"
     gene            complement(3217..4608)
                     /locus_tag="OO357_003868"
     CDS             complement(3217..4608)
                     /locus_tag="OO357_003868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HCR5888633.1"
                     /translation="MTTPVWRNDDLEGAVIGAFFLRGADPEVMDILATLPADVFSVRA
                     YQDIYTGICRQARVSGVIDPVLLCNEMPELAPVITDTGRKTWVKSSLEHYVAALRRNA
                     ALRDAEKTLNEALQKLRDAHTCEAAEDALKDAQNMMVTLSTGKGVIQPVHIDDVLPEV
                     VERVECRNQGLEKSRTLMTGIDELDAKTGGMEPGDLVFIAARPSMGKTELALDIIDKV
                     TEQGHGVLLFTMEMANIQIGERMVSAAGGMPVSRLKSVAHFEDEDWTRFSQGVGRMTG
                     RNIWMVDQANLAIDEICATTKHHLIKYPETALVVVDYLGLIKTRTTGRHDLAVGEISK
                     GLKGLAKSGGFPLIALSQLSRGVESRPNKRPMNSDLKNSGEIEADADIILMLYRDEVY
                     NPDTQARGIAEINITKQRNGSLGTIYRRFYNGHFLPVDQESARVLSTPMKPGNPRRYS
                     NKRTDSSKMERFF"
     gene            complement(4605..5483)
                     /locus_tag="OO357_003869"
     CDS             complement(4605..5483)
                     /locus_tag="OO357_003869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5888634.1"
                     /translation="MMTFNLREQQKRLQARMDELRAEIAFAQKGEKPWPYRSCLMREG
                     RGYCEKHGEYHTHILVWSDRNGEDREEISCCPDCLIAEANDLTMELSSIKAEELTDNA
                     GIALRFRDCEFDNYLEVNPGAARNLAACRRYAENWPDMLENGTSLVMTGSCGTGKNHL
                     AVAMAKHIIRNYLASVEITDVMRLTRAVKNCWRNDSEKTADEVIERYASMDLLIIDEV
                     GVQFGSAAEMAILQEIINARYESILPTILISNLSPEELWAFISPRIADRITDGGRNWL
                     SFNWPSYRSRIRGVAA"
BASE COUNT         1329 a         1541 c         1201 g         1420 t
ORIGIN      
        1 gatgatgcag ataaccagag cggagataat cgcggtgact ctgctcattg atctatcccc
       61 caacaggcta atgcgctttc ctggtcacga cgaataacct gtccatagca gttatttgaa
      121 cgtatgcggc aatcgcgccc aacatctttt atccaccagc gaatcgcctc gcatgcgccc
      181 ttacgatcac cggcattcag ccgcttataa aacgtcgacg ggaaacactt accggggcca
      241 atgttatagg ggcaaaatga cgctataccc gctttctggg gttcggtcag aggtacttta
      301 atattgcgct ccacccatgc cagcgcctta tcacgttcaa tagcgttaac ctggtcacat
      361 ttttccttcg acagcttcat tcccggtatg acaggcttac catccaccat tgtggcacca
      421 cgacagatgg tccatatgcc agaaccatca cggtatgccg ttgtgtgatt accctctttt
      481 tcgttcagaa actggtcaag tatctgggga gcagacgcgc ctgcggcaat cagcgccaga
      541 acagcagccg acaggccgta tttgattttt gcgctcatgg atatttatca ggatttatcg
      601 gcaacaaata acgagccaac ttatatacgc cttttaagat aagtcagccc cggctggatc
      661 cagtcatcag gctctttctt aaagggggag tattgaaatc acgaagaaga gcctcccgca
      721 ccgcagcatc catgtctgca ccactggcaa gtgcttcaat ctctgccgcc acctgcaaat
      781 accccatgca acggccaatg cgtttcataa ctctggtgtt ttcgtctttc aggcatctga
      841 tagcgagatt aataattttc aggcagtgat gatgcgatgc ccggacaggt tttccgttct
      901 cataaacgac gaactcaccc tcttcacagg aaaccctgaa aggccgatgg ggttcctggt
      961 ctttatccgg tgcagacgca ataaaatcac ttttatgctc ttcctctccg gcactgttaa
     1021 ccgtaatgac gtactcaggc cgcaggtaag ccgcctcttt ttctgacaat gcggccggta
     1081 aaaacttttc ctgcaaaaac ttcataaaag tctcgtgagc gactttgtgg cagtaatcgt
     1141 aaatcgccgc ataatgctca tcgcggcgtt tgttttcatc ttcagaactc accaacgccg
     1201 acaatttttt attaagctca gtgatttcat tttccaggtg attgaatcgc tgattcattt
     1261 cttcatgctt cattatttac tctccccgtg cagccttacg cttgtcttct cttattttga
     1321 aatacagatt cgtcagataa gtcagaagcc ccagaagcag acttcccagc acaccaatcg
     1381 cagcccactg tgatggcctg acctgatcca accactgcaa aaaccagtag ccggcactgc
     1441 cggcggaggt gccgtaggca atgcccgttg aaattttgtc catggatttc atagcctcac
     1501 ctccgcaaat aacggatggc gtagttttac actgagaaat gaaagggatt tgaaaagaaa
     1561 aaaacgcaaa agcgggcgaa acgatatata cagtaaggaa agcactctat ccaacaaacc
     1621 acccacagtt aatcggaata aaagcagagt gcttatgaat gatcgcacgc ccgaaggtta
     1681 gtatttctgc acagcaattt tgcaaaaaaa ggcgatcatt cataacttaa acgtctttca
     1741 gtcactccgg gatttcccat catcgcagac tgaaagactc tgactggagc gggcagcggg
     1801 aatcgaaccc gcatcatcag cttggaaggc tgaggtaata gccattatac gatgcccgca
     1861 tatggtgccg actaccggaa tcgaactggt gacctactga ttacaagtca gttgctctgc
     1921 ctgctgagct aagtcggcgc tggcccacca ccgaggactc gaacctcgca ccgtcaactt
     1981 agaaggttga tgctctatcc ggatgagcta gtggtggttg gtggcccttg ctggacttga
     2041 accagcgacc tggcgattat gagtcgctcg ctctgaccaa ctgagctaaa gggccggagg
     2101 cagaataata accatatgtc atcacatctg caaactcatc tgaccaccag cgtgtttaac
     2161 gtcctgtacc gtttttcagg cataaaaaac ccgcccggag gcgggtttaa gctgtgtggc
     2221 gaagcaacca ctcttaacag aatatcccgt tttttacgta cgtaaaatat tttctagaaa
     2281 gtcgcccctt accatcaggg atgttcaata tatttgtcca tttctaaccg gacacccaac
     2341 atcatcaaca ttccttccac caccccctcg gctttttgta gcttctttcc tatataacca
     2401 tctgagcatc catgcttccg cgccagcgtc atgaatgtca tcccaaacac gtagtagtca
     2461 accagcaagt catgtagatc gttgttgttc cggttaaggc gggccataca cccacatata
     2521 accatcgcgt catcgtcaca gcattgcggg cgggatttta cttttgaagg gattaatccc
     2581 ttaaaaccgg cagcaatgga cgaccaggtc acatcctcat gattattagc cacccacgcg
     2641 ccccagcgct caagaacctg ctgaatatca cgcatcactg tctttacccc tgtcccatcc
     2701 acgatgaaca atcagaacac catcaacaat ggcgtgccct ttgccctctt tatcttcagc
     2761 atattttctt accgtggcgc gattacagtt cagcattcgt gaaacttcgg tcatattacc
     2821 tcgtgtctgg ataagcagtt ccggtatcgt ttgaattttg acgctcatca aatactctcc
     2881 ggttcggtga tttttatccc gggctttcca ccagggacat aatgatcgcg cacaagccag
     2941 ggcaaaacaa actcatggcg catcagcgca gcgcctcctg caccagtttt tcaaactttc
     3001 cgactctggt ttccagctct gccacacagt ccaccagctc atctactgct ttctgtgcgc
     3061 gatgcttcgc ctgcatcagt tcccgaagcg cgggtaccat atctttacgg atagcatctt
     3121 ttgttatgcc cgttttttcg agttgttcag catgacgcag catttcctgc gcgtgtttac
     3181 gcaattgttc aggggtaaaa gtcattgtct ggttgttcaa aagaaacgct ccatcttact
     3241 gctgtcagtt cgtttattac tgtatctgcg cggattgccg ggcttcatgg gagtggaaag
     3301 cacccgtgca ctttcctggt ccacaggcag aaaatgtccg ttataaaaac gccggtaaat
     3361 cgttcccaga gaaccgttac gttgtttcgt gatattgatt tctgcgatgc ccctggcctg
     3421 cgtatccggg ttgtacactt catccctgta aagcatcaga atgatgtctg catccgcctc
     3481 tatttctccg gaatttttca ggtctgagtt catgggacgt ttattgggtc tggactccac
     3541 accgcgggag agctggctca gcgcaatcaa cggaaaacca ccggattttg ccaggccttt
     3601 aagccccttt gagatttcac ccacggcaag gtcatgacgc cccgtggttc gggtttttat
     3661 cagcccgaga taatcaacca ccaccagcgc cgtttccgga tatttaatca ggtggtgttt
     3721 cgttgttgcg catatctcat caatggccag gttcgcctgg tccaccatcc agatattgcg
     3781 cccggtcatc cgccccaccc cttgtgagaa acgcgtccag tcttcatctt caaagtgagc
     3841 cacagatttc aggcgtgata ctggcatccc tccagccgca gacaccatac gttcaccaat
     3901 ctggatgttc gccatctcca tggtgaacag aagcacacca tgcccctgct cagtcacctt
     3961 gtcgatgatg tccagcgcaa gttcggtttt ccccatcgaa ggacgggcgg caatgaatac
     4021 caggtctccg ggctccatac cgcctgtttt tgcgtccagt tcatcaatac cggtcatcaa
     4081 cgtcctggat ttctccagcc cctgattccg gcattcaaca cgctcaacca cttccggaag
     4141 cacatcatca atatgtaccg gctgaatgac gccctttcct gtcgacagtg tgaccatcat
     4201 gttctgcgca tccttcaggg catcttcagc tgcttcacag gtatgcgcat cacgtaattt
     4261 ctgcagcgcc tcattcagtg ttttttctgc atcgcgcagt gcggcattgc gccgcaacgc
     4321 tgcaacatag tgctccagtg aagacttcac ccaggtttta cgcccggtat cagtaatcac
     4381 cggggcaagt tccggcatct cattacacaa cagcacgggg tcaatcactc ctgaaacacg
     4441 ggcctgtctg cagatgcctg tatagatatc ctgatacgct cgtacagaaa aaacgtccgc
     4501 tggcagtgtg gccagaatat ccatcacttc aggatctgct ccgcgcagaa agaacgcgcc
     4561 gatgacagcg ccttcaaggt catcgttacg ccatactgga gttgtcatgc agccacacct
     4621 ctgatacgag aacggtaact gggccagtta aacgacaacc agttgcgtcc cccgtctgtg
     4681 atcctgtcgg caattcgggg actgatgaac gcccacaact cttccggtga gaggttactg
     4741 atcagaatgg tgggcaagat actttcgtac ctggcgttga taatttcctg caaaatagcc
     4801 atttcagccg cgctgccaaa ctgaacgcca acttcgtcga tgatcagcaa atccattgac
     4861 gcataacgct caataacttc atccgctgtt ttttcgctgt cattccgcca gcaatttttc
     4921 acagcacggg taaggcgcat cacgtcggtg atctccacac tggccagata gttacggatg
     4981 atgtgttttg ccatagccac agccagatga ttttttccgg taccacaact gccggtcata
     5041 acaagactgg taccgttctc cagcatatct ggccagttct ccgcatagcg gcgacaggcc
     5101 gcaagatttc tggctgcgcc aggattaacc tccagataat tatcaaactc gcagtcccga
     5161 aaacgcaggg caattccggc gttatcagtc agctcttccg ccttgatgga cgacagctcc
     5221 atggtcaaat cgttggcctc agcgattaag cagtcagggc agcatgaaat ttcttctctg
     5281 tcctcgccat tacgatcgct ccacaccagt atatgcgtgt gatattcgcc atgtttttcg
     5341 caatatccgc gaccttcacg catcaggcag gaacgataag gccatggctt ttcgcccttc
     5401 tgagcaaatg caatctctgc ccgtaactca tccattcgcg cctgtagtct tttttgttgt
     5461 tcacgcaggt taaacgtcat catcgctgtc a
//
LOCUS       DAJTRC010000173         5485 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.175, whole genome shotgun sequence.
ACCESSION   DAJTRC010000173 DAJTRC010000000
VERSION     DAJTRC010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.175"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..1630)
                     /locus_tag="OO357_003870"
     CDS             complement(<1..1630)
                     /locus_tag="OO357_003870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional alpha/beta hydrolase/class I
                     SAM-dependent methyltransferase"
                     /protein_id="HCR5888635.1"
                     /translation="MENSRIPGEHFFTTSDNTALYYRHWPTLQPGAKKVIVLFHRGHE
                     HSGRLQHLVDELAMPDTAFYAWDARGHGKNSGPRGYSPSLMRSVQDVDEFVRFAASDS
                     QVGLEEVVVIAQSVGAVLVATWVHDYAPAIRGLVLASPAFKVKLYVPLARPALALWHR
                     LRGLFFINSYVKGRYLTHDRQRVASFNNDPLITRAIAVNILLDLYKTSERIVSDAAAI
                     TLPTQLLISGDDYVVHRQPQIDFYQRLRSPLKELHLLPGFYHDTLGEENRAQAFEKMQ
                     SFICRLYANKSQKFDYQHEDRTGPSADRWRLLSGGPVPLSPVDLAYRFMRKAMKLFGA
                     HSAGLHLGMSTGFDSGSSLDYVYQNQPQGSNAFGRLIDKIYLNSVGWRGIRQRKTHLQ
                     ILIKQAVADLHAKGLAVRVVDIAAGHGRYVLDALANEPAVSDILLRDYSELNVAQGQE
                     MIAQRGMSGRVRFEQGDAFNPEELSALTPRPTLAIVSGLYELFPENEQVKNSLAGLAN
                     AIEPGGILIYTGQPWHPQLELIAGVLTSHKDGKPW"
     gene            complement(1646..2530)
                     /locus_tag="OO357_003871"
     CDS             complement(1646..2530)
                     /locus_tag="OO357_003871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005142702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HCR5888636.1"
                     /translation="MSGNTFRVVNFSKLINRFLLWRLPERKGDEVTLRIRTWWGIVIC
                     FSLVISGPRWMTLTFFTLISFLALKEYCTLISVHFSRWLYWVIPLNYLLIGFNCFELF
                     LLFIPLTGFLILATWRVFVGDPSGFQHTVSAIFWGWIMTVFALSHAAWLLMLPTINIQ
                     GGALLVLFLLALTESNDIAQYLWGKSCGRRKVVPKVSPGKTLEGLLGGVITTMIASLI
                     IGPLLTPLNTLQALLAGLLIGISGFCGDVVMSAIKRDIGVKDSGKLLLGHGGLLDRID
                     SLIFTAPVFFYFIRYCCY"
     gene            complement(2541..3146)
                     /locus_tag="OO357_003872"
     CDS             complement(2541..3146)
                     /locus_tag="OO357_003872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5888637.1"
                     /translation="MTLYQIKPLFQSLLRPTMFWLYKHHVTANHITLTALALSLFTGL
                     LLVLVAQPILFLLLPIVLFIRMALNALDGMLARECNQQTRLGAILNETGDVISDIALY
                     LPFLFLPESNASLVILMLFCTILTEFCGLLAQTINGVRSYAGPFGKSDRALIFGLWGL
                     AVAIYPQWMQWNNLLWSIASILLICTAINRCRSVLLMSAER"
     gene            complement(3317..>5485)
                     /locus_tag="OO357_003873"
     CDS             complement(3317..>5485)
                     /locus_tag="OO357_003873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415925.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2773 domain-containing bactofilin"
                     /protein_id="HCR5888638.1"
                     /translation="KQQVEQAINSSVNLVPFGLSASDWKVHRGDLVVEGNIESNQKLI
                     VLGNLTVKGNISTFSLSNPWVILGNVTATNIVTDSPLLITGSINASGLVFIDSYYDNP
                     STIKGSINARGVFINDIIAPVVASSTNSEFMVRASDKNDTENVKKALMIINPDAYYWG
                     LINDEDALKEIFKRSNIRMAGNVCNQMKKEALFRPKPSPELVQELQMLDEGNVAAFEG
                     RDIATFDLAIMRTLPRLKGISANLRKQLINSNDEQTIESMARYMPDNEILELTDQQLG
                     YQPVVLGLLDREPLSVEIMTRMSRLPDGVGPLNLALRENLPLDIVMTLAKRDWDMIIQ
                     ELYKDAWLLPESIIDGYIRSDDSSIRQVGAGGQLTYNQAMQLANDSSNNVVTSLAFKL
                     AEMKHHGQLLRMTPQESDKVAAYLYQKFENDDDLIRVLFLALPDNLQFNFVKRMEKKS
                     PAYFCCRDMQVIHSDAALQRLLTRFNDPEGWSNLAKNQYLSTSMKQKIWQRALSHRKN
                     NPKADSDAYETSADMILSELISHGEVDDQMLLNAAALIRLEDWDFLESALVSWDNLPA
                     VVLKELQQNTPRNDIWAKFFLRQENSSRAQVDEALRVYYALDPDALAQLDVLAKQPDR
                     IWWSTLAKSNLTFFKFGALSNRHTPSAALAAEIDPEWWIVAMNNPRFPVDVLKARLKR
                     DPLLALELVNPELDLVRQLALNGKTRAIREQAMRKLDELY"
BASE COUNT         1581 a         1364 c         1141 g         1399 t
ORIGIN      
        1 cccacggttt accatcttta tgactggtta acaccccggc aatcagctcc agttgtgggt
       61 gccacggctg cccggtgtag atgagaatgc cacccggttc gatggcattg gctagacctg
      121 cgagtgagtt ttttacctgc tcattttcgg gaaaaagctc atataggcca gagacaatcg
      181 ccagcgtagg ccgcggagtt aacgcgctga gttcctccgg gttaaacgca tcgccctgtt
      241 caaaacgcac ccgcccagac attccccgtt gggcaatcat ctcttgcccc tgtgcaacat
      301 ttaactcact gtaatcacgt aacaaaatat cgcttacggc aggctcgttt gccagcgcat
      361 ccagtacata gcgtccatgc cctgcggcaa tgtcaaccac gcggacggct aaacctttgg
      421 cgtggagatc ggcaacggct tgtttaatca atatttgcaa atgggttttg cgctggcgaa
      481 taccgcgcca tccaacactg ttcaggtaga ttttgtcgat taaacgcccg aatgcgttac
      541 tgccttgcgg ttgattttga tagacataat ccagggaact gcctgaatca aagccggtac
      601 tcattccgag atgcaggccc gcagagtgcg caccgaacaa tttcatcgcc tttcgcataa
      661 agcgatacgc taaatcaacc ggcgataatg gcacgggtcc accagaaagg agccgccatc
      721 gatccgctga tggtcctgtg cggtcttcat gctgataatc aaatttttgc gatttgttag
      781 catataagcg gcagataaag ctttgcattt tttcaaatgc ctgcgccctg ttctcttcac
      841 ccaatgtgtc gtgataaaag cctggcagca gatgcagctc tttcagaggg ctacgtaatc
      901 tctgataaaa atcaatctgc ggttggcgat gcaccacata gtcatcacct gatatcagaa
      961 gttgcgtggg gagcgtaatc gccgccgcat cgctaacaat acgttcagac gttttataga
     1021 gatcgagcaa gatattgacg gcaatcgccc gtgtgatcag cggatcatta ttgaaactcg
     1081 ccacccgttg ccgatcgtgg gtcaaatagc gccctttcac ataggaatta ataaaaaata
     1141 gaccacgcag acgatgccat aacgccagcg caggacgtgc cagcggcaca tacaatttaa
     1201 ccttaaaggc cggagaagcc agcaccagcc cacgaattgc aggtgcataa tcatgaaccc
     1261 atgtggcaac aagcactgcg ccgacgcttt gcgcgatcac taccacctct tccagtccga
     1321 cctggctgtc gctggcagca aaacggacaa attcatcgac atcctgcact gagcgcataa
     1381 gagacgggct ataaccacgc ggccccgaat tttttccatg cccccgggca tcccaggcat
     1441 aaaaagcagt atcaggcatc gccagttcat caacgagatg ttgtagacga ccagaatgtt
     1501 catgcccgcg atgaaataag acgatgacct ttttcgcccc tggctgtaaa gtgggccagt
     1561 gccgataata caatgctgta ttatcactgg tggtaaaaaa atgttcccca gggatgcgtg
     1621 aattttccat tttctctatt ttccttcagt aacagcagta gcgaataaaa taaaaaaata
     1681 ccggagcggt gaaaattaat gagtcaatcc tgtcaaggag tccgccatgt cctagtaata
     1741 gttttccact atctttaaca ccaatatctc gtttgatggc tgacatcact acatcgccgc
     1801 aaaaaccact aataccaatt aacaaacccg ctaataatgc ctgtaatgta tttagcggtg
     1861 tcagcaacgg tccaataatc agtgacgcaa tcatggtggt gattacgccc cccagcagac
     1921 cttccagcgt ttttcccggg ctgactttgg ggaccacttt tcttctgccg caggatttcc
     1981 cccataaata ctgtgcaata tcgtttgact cggttaacgc caaaagaaat aacaccagta
     2041 acgccccgcc ctggatattt atggttggca acattaatag ccaggcggca tgactcaagg
     2101 caaaaacggt cattatccat ccccaaaaaa tggcgctcac ggtatgctga aaaccggagg
     2161 ggtcacccac aaaaactcgc caggtggcta atatcagaaa cccagtcaaa ggtataaata
     2221 acagaaacag ctcaaagcaa ttaaaaccga taagcaaata gttaagagga ataacccaat
     2281 ataaccaacg cgaaaaatgt acggatataa gcgtacaata ttctttcaat gccagaaaac
     2341 ttatcagcgt aaaaaacgtc aacgtcatcc agcgtgggcc tgaaatcacc agtgaaaaac
     2401 aaatgacgat gccccaccag gtacgaatac gtaatgtaac ttcatcccct tttctctcag
     2461 gtaaccgcca tagcagaaaa cggtttatca gcttgctaaa attaacaacg cgaaaagtgt
     2521 tgccagagat ttttccagca ttatctttca gcgctcataa gaagcacact ccgacaacga
     2581 ttaatcgcag tacagataag cagaattgaa gcaatgctcc ataaaagatt attccattgc
     2641 atccactgtg gataaatggc aacggccaga ccccacagac caaatattaa cgcgcgatca
     2701 cttttgccaa atggaccggc ataactgcga acaccgttaa tcgtctgcgc gagtaaaccg
     2761 caaaactcgg tcaatatggt gcaaaataac atgagtatca cgagtgatgc gttactttcc
     2821 ggtaaaaata aaaacggtaa atagagcgca atatcggaaa taacatcgcc agtctcattc
     2881 aaaatcgccc ccagacgtgt ttgctgattg cactcacgcg ccaacatgcc atccagcgca
     2941 ttgagcgcca tacggataaa aagcacgatg ggcaatagca aaaagaggat gggttgtgcc
     3001 accaacacca acagcaatcc ggtaaaaaga gaaagcgcca gtgccgtaag agtgatgtga
     3061 ttcgctgtaa cgtggtgctt ataaagccaa aacatcgtcg gccttaacag cgactgaaag
     3121 agcggtttta tctggtagag cgtcattatc aatccttgat aattcggttt gcgctatggt
     3181 gcatcaattc ccctgacgga gatatcgcaa cacgccgctt cattaaaaat tcagaatata
     3241 tcaaggtatt gggggctcac tgtcaggaat gattatcccc aataaatcaa gtagttaaaa
     3301 attattacgc tgttattcaa tacaactcat caagtttcct catcgcttgt tcccgtattg
     3361 cgcgcgtctt accgttaagc gccagctggc ggactaaatc cagttcaggg ttaacaagct
     3421 ctaacgccag caaaggatca cgtttcagcc tcgccttcaa tacatcaact ggaaagcggg
     3481 gattattcat cgccacaatc caccactcgg gatcaatttc tgccgccagt gccgcagagg
     3541 gggtgtggcg gttgctaagt gcgccgaact tgaaaaatgt gagattgctt ttcgccagtg
     3601 tactccacca tatacgatcc ggttgttttg ccagtacatc cagttgtgct aacgcatcgg
     3661 ggtcgagtgc ataataaaca cgtaatgctt catcaacctg cgcacgggag ctattttcct
     3721 gcctcagaaa aaacttcgcc caaatatcat tgcgtggcgt gttttgctgt aattccttga
     3781 gaacaacagc gggtaaatta tcccaactaa ctaatgcact ctctaaaaag tcccagtctt
     3841 ccaaacgtat cagggcggcg gcatttaata gcatctggtc atcaacttcg ccgtggctaa
     3901 tcagctcaga caaaatcata tcagcgctgg tttcatatgc atctgaatct gctttcggat
     3961 tatttttccg atgcgataaa gcacgttgcc aaattttttg tttcatcgac gtgctcagat
     4021 actgattttt cgccagatta ctccacccct caggatcgtt gaaacgtgtc aataaccgtt
     4081 gtaaagcagc gtcagagtga attacctgca tatcccgaca gcaaaagtag gcaggggatt
     4141 ttttctccat ccttttaaca aaattaaact gtaagttatc tggcaatgct aaaaataaca
     4201 cacgtattag atcatcgtca ttctcgaatt tttggtataa ataggctgca actttgtcac
     4261 tctcttgtgg cgtcatccgc aataattgac catggtgttt catctctgcc agcttgaacg
     4321 ctaagcttgt gacaacattg tttgatgaat cgttcgccaa ctgcattgcc tgattgtaag
     4381 tgagttgtcc tccagcaccg acctgacgaa tagaggaatc atcactgcgg atatagccat
     4441 caataatgga ttcgggcaat aaccacgcat ctttataaag ctcctgaatg atcatatccc
     4501 aatctctttt cgccagagtc atgacgatat ccagagggag attttcacgt agcgcaagat
     4561 tcaacggacc gacaccatca ggtagacgac tcattcgcgt cattatttcg acagagagcg
     4621 gttcacggtc gagcaacccc agaacaacag gctggtaacc caattgctga tcggtcaact
     4681 ccagaatctc attgtcaggc atatatctcg ccatactttc aatcgtttgt tcatcattgc
     4741 tattaataag ttgtttgcgt aggttagctg aaattccttt aagccttggg agagttcgca
     4801 ttatggcaag atcaaatgtc gcaatatctc gtccttcaaa tgcagcaacg ttgccttcat
     4861 ccagcatttg caattcctgc actaactcag gagaaggctt agggcgaaac agcgcttctt
     4921 ttttcatctg attacagaca ttccctgcca tgcgaatatt gcttcgctta aaaatttctt
     4981 tcagagcatc ttcatcattt attagacccc aataatatgc atcaggattt attatcatca
     5041 gcgctttttt gacattttca gtgtcatttt tgtcactcgc acggaccatg aattcactat
     5101 ttgtcgaaga cgcaacaaca ggtgcaatta tgtcattgat aaatacccca cgcgcattaa
     5161 tactcccctt aatcgtagac ggattatcgt aatatgagtc gataaatacc agtccacttg
     5221 catttatcga accggtaatt aacagtggtg aatccgtaac tatattcgtt gccgtcacgt
     5281 taccgagaat aacccatgga ttagaaagag agaaagtgga aatattacct ttgactgtca
     5341 aattgccaag aacaatcaat ttttgattac tctctatatt accttctacc actaaatcgc
     5401 cacgatgcac tttccagtcc gatgcagata aaccgaaggg gacaagattc acgctactgt
     5461 tgatagcttg ttcaacttgt tgttt
//
LOCUS       DAJTRC010000174         5470 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.176, whole genome shotgun sequence.
ACCESSION   DAJTRC010000174 DAJTRC010000000
VERSION     DAJTRC010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5470)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5470)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5470
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.176"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..246
                     /gene="mddA"
                     /locus_tag="OO357_003874"
     CDS             <1..246
                     /gene="mddA"
                     /locus_tag="OO357_003874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707633.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-methionine sulfoximine/L-methionine sulfone
                     acetyltransferase"
                     /protein_id="HCR5888639.1"
                     /translation="QGKGLGRKLLSRLIDEARDCGKHVMVAGIESQNQASLHLHQSLG
                     FVVTAQMPQVGTKFGHWLDLTFMQLQLDERTEPDAIG"
     gene            243..692
                     /locus_tag="OO357_003875"
     CDS             243..692
                     /locus_tag="OO357_003875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HCR5888640.1"
                     /translation="MNQSLTLAFLIAAGIGLVVQNTLMARITQTSSTILIAMLLNSLV
                     GIVLFVSILWFKQGMAGFGELVSSVRWWTLIPGLLGSFFVFASISGYQNVGAATTIAV
                     LVASQLIGGLVLDIFRSHGVPLRALFGPICGAILLVVGAWLVARRSF"
     gene            complement(693..926)
                     /locus_tag="OO357_003876"
     CDS             complement(693..926)
                     /locus_tag="OO357_003876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcY family protein"
                     /protein_id="HCR5888641.1"
                     /translation="MSHLDEVIARVDAAIEESVIAHMNELLIALSDDAELSREDRYTQ
                     QQRLRTAIAHHGRKHKEDMEARHEQLTKGGTIL"
     gene            complement(1012..1185)
                     /gene="ortT"
                     /locus_tag="OO357_003877"
     CDS             complement(1012..1185)
                     /gene="ortT"
                     /locus_tag="OO357_003877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415962.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="orphan toxin OrtT"
                     /protein_id="HCR5888642.1"
                     /translation="MFLYQHMLVFYAVMAAIAFLITWFLSHDKKRIRFLSAFLVGATW
                     PMSFPVALLFSLF"
     gene            1380..1475
                     /gene="yncL"
                     /locus_tag="OO357_003878"
     CDS             1380..1475
                     /gene="yncL"
                     /locus_tag="OO357_003878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001165318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stress response membrane protein YncL"
                     /protein_id="HCR5888643.1"
                     /translation="MNVSSRTVVLINVFAAVGLFTLISMRFGWFI"
     gene            complement(1877..2686)
                     /locus_tag="OO357_003879"
     CDS             complement(1877..2686)
                     /locus_tag="OO357_003879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CatB-related O-acetyltransferase"
                     /protein_id="HCR5888644.1"
                     /translation="MKVQLYKFTEDKNKTLTFRWTKKHFEFCMDNKIFLNHKGKKSYK
                     ERNLFLFSKGDKITIEDNVIAEEYSTMPVKNFSSVGAFSFPTCHFSGNIRIGRFCSIA
                     SNVKIMGGNHPLNRFTTHMMTYNGEFDKFAMSEFERSWTLKPFITKPENPIIGNDVWI
                     GNDVVLKGGIAIGDGAVIAANSVVTKDVPPYAIVAGVPAKIIRFRFDSNVIDELLRIK
                     WWNYNYSDLPDNNKCDDINYFVEEMNRLISNGNIQERDYKKFNLSEVFRGL"
     gene            complement(2896..4320)
                     /gene="patD"
                     /locus_tag="OO357_003880"
     CDS             complement(2896..4320)
                     /gene="patD"
                     /locus_tag="OO357_003880"
                     /EC_number="1.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminobutyraldehyde dehydrogenase"
                     /protein_id="HCR5888645.1"
                     /translation="MQHKLLINGERVSGEGEKQPVYNPATGDVLLEIAEVSAEQVDAA
                     VRAADAAFAEWGQTTPKARAECLLKLADVIEENGQVFAELESRNCGKPLHSAFNDEIP
                     AIVDVFRFFAGAARCLNGLAAGEYLEGHTSMIRRDPLGVVASIAPWNYPLMMAAWKLA
                     PALAAGNCVVLKPSEITPLTALKLAELAKDIFPAGVINILFGRGKTVGDPLTGHPKVR
                     MVSLTGSIATGEHIISHTASSIKRTHMELGGKAPVIVFDDADIEAVVEGVRTFGYYNA
                     GQDCTAACRIYAQKGIYDTLVEKLGAAVATLKSGAPDDESTELGPLSSLAHLERVSKA
                     VEEAKATGHIKVITGGEKRKGNGYYYAPTLLAGALQDDAIVQKEVFGPVVSVTPFDNE
                     EQVVNWANDSQYGLASSVWTKDVGRAHRVSARLQYGCTWVNTHFMLVSEMPHGGQKLS
                     GYGKDMSLYGLEDYTVVRHVMVKH"
     gene            complement(4342..5136)
                     /locus_tag="OO357_003881"
     CDS             complement(4342..5136)
                     /locus_tag="OO357_003881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HCR5888646.1"
                     /translation="MHSERAPFFLKLAAWGGVVFLHFPILIIAAYAFNTEDAAFSFPP
                     QGLTLRWFSVAAQRSDILDAVTLSLKVAALATLIALVLGTLAAAALWRRDFFGKNAIS
                     LLLLLPIALPGIVTGLALLTAFKTINLEPGFFTIVVGHATFCVVVVFNNVIARFRRTS
                     WSLVEASMDLGANGWQTFRYVVLPNLSSALLAGGMLAFALSFDEIIVTTFTAGHERTL
                     PLWLLNQLGRPRDVPVTNVVALLVMLVTTLPILGAWWLTREGDNGQ"
     gene            complement(5126..>5470)
                     /locus_tag="OO357_003882"
     CDS             complement(5126..>5470)
                     /locus_tag="OO357_003882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5888647.1"
                     /translation="ILPVQAALERLPPSLLQASADLGARPRQTFRYVVLPLAIPGIAA
                     GSIFTFSLTLGDFIVPQLVGPPGYFIGNMVYSQQGAIGNMPMAAAFTLVPIILIALYL
                     AFVKRLGAFDAL"
BASE COUNT         1448 a         1400 c         1198 g         1424 t
ORIGIN      
        1 cagggcaaag gtctggggcg taaattgtta agtcgattga ttgatgaagc gcgggattgc
       61 gggaagcatg tcatggtcgc cgggatcgaa tcgcaaaatc aggcttcgct gcatctgcac
      121 cagtcgctgg gatttgtcgt caccgcacaa atgccgcagg taggcactaa atttggtcat
      181 tggctggatc tgacatttat gcagttgcaa ctcgacgagc gcactgaacc ggacgcgatt
      241 ggatgaatca gtcgctcacc cttgcttttt tgattgccgc cggaattggt ctggtagtcc
      301 agaacacgct gatggcgcgc atcacccaga cctcctctac cattctcatc gccatgttgc
      361 tgaactcact ggtggggatt gtgctctttg tttctatttt atggtttaaa caaggcatgg
      421 cggggtttgg cgaactggtg tccagcgtgc gctggtggac acttatacct ggcttgctgg
      481 gttcgttttt tgtcttcgcc agtatcagcg gatatcagaa tgtgggagcg gcgacaacta
      541 ttgcggtact ggtcgccagc cagctgattg gcggtctggt gctggatatc ttccgcagtc
      601 atggcgtacc actacgggcg ttgttcggcc caatatgtgg cgcaatttta cttgtggttg
      661 gcgcctggtt ggtggccaga cgctcatttt aatcagagga tggtgccgcc tttggttaac
      721 tgttcgtggc gcgcttccat atcttcttta tgcttgcgac cgtgatgagc aatagcggtg
      781 cgcaaacgct gctgctgggt gtagcgatct tcccgactta actccgcgtc atcgctcagg
      841 gcgattaata attcgttcat atgggcaatg acgctctctt caatcgcggc atccacgcgc
      901 gcgatgactt catccagatg cgacatactt tctccttagc cacttgtgaa atttatcgat
      961 taatccaaat gatataagtc tgcgtggtgc cgtcaagcca gtgagtaaga tttaaaacag
     1021 tgaaaacaac agcgccaccg ggaaactcat cggccatgtg gcccccacca gaaaggcgct
     1081 taagaaacgg atgcgtttct tatcgtgaga aagaaaccag gtgataagaa atgcgattgc
     1141 tgccataacc gcataaaaaa caagcatatg ttgatagaga aacatgtacg ggcgtccagc
     1201 catatgaata aaacgcgcgc aaatatgcgc cattttttga cgtgcatcaa gttttttcac
     1261 cagtttgaga aaaaatcatc aaacagcaac ttgcccttat ccatcttcag aatgagtatt
     1321 attgcggatt ttcttaaccc gtactataca cataggggca atgataaaag gtggcaaaaa
     1381 tgaatgtttc cagtagaact gtagtactga taaatgtctt tgctgctgtt ggtttgttta
     1441 ctcttatctc tatgagattt ggctggttta tttgatgtag tgattttgca cgcagcacgt
     1501 actgtcagag aaatcaactc tctgacagta cgcaagatct tctcatactc tccaagtttg
     1561 caacattatc cttaatattt acttcttttt gctggcatct acattgaaat gagtagaaaa
     1621 catcccccta ctgtcgtcta tacaaacagc ttactgattc aaacggaatc ttgttttcac
     1681 atttttctat cacgaatact ttatacgcgc gggagtctat aaatgcttta tactttcaag
     1741 gaataacgta aatataaaaa gatggcgtaa agtatatgca tggcaggatg tttgcaacct
     1801 ccttgatgac gctgccccat tgaaattgca aaccccgccc tggttatctt gcccatttat
     1861 catgcaaaac agataattat aggcctctga atacctcaga aagattgaat tttttatagt
     1921 ccctctcttg aatatttcca tttgatatca atcgattcat ctcttcaaca aaataattta
     1981 tatcatcaca cttattatta tccggaagat ctgagtagtt ataattccac cattttattc
     2041 tcaacagttc atcaataaca tttgaatcaa acctaaatct gattattttt gcaggaactc
     2101 ctgcaactat ggcatatggg ggcacatctt tggtaacaac ggaattggca gcaataacag
     2161 caccatcacc tattgcgatg ccacctttca atacaacatc atttcctatc cagacatcat
     2221 ttcctataat cggattttca ggtttggtaa tgaatggttt caatgtccag ctacgttcaa
     2281 attcactcat agcaaactta tcaaattcac cgttataagt catcatgtga gtagtaaaac
     2341 gattcaaagg atgatttcca cccattattt ttacatttga tgcaatacta caaaatcgac
     2401 ctatacgtat attccctgaa aaatggcagg tcggaaatga aaaagcgccg acactggaga
     2461 aatttttcac aggcattgtt gaatattctt cagcaataac attatcttca attgttatct
     2521 tgtcaccttt tgagaaaaga aataaattcc tttctttata cgacttcttc cccttatgat
     2581 ttaaaaagat tttattatcc atacaaaact caaagtgttt tttcgtccat ctgaaggtaa
     2641 gggttttatt tttatcttcc gtgaatttgt acaattgaac tttcattgtt aatacttcta
     2701 cttataaagt attagtttaa taacgatact ctaaagcctt atgctaatca actgtgacag
     2761 gatgaaaaat cgccatcaaa catttagcct aatttttacg ccagttatta atgtaatcat
     2821 tctaatactt aaccgccatt aaactcagaa cctaaaacga tagtggccgt cctataacag
     2881 ccactaacat ctaacttaat gtttaaccat gacgtggcgg acgacggtgt aatcctccag
     2941 cccataaagt gacatatcct tgccgtaacc agaaagtttc tgcccaccgt gcggcatttc
     3001 acttaccagc atgaaatggg tattgaccca ggtacaacca tattgcagcc gtgcgctgac
     3061 gcgatgcgcc ctgcccacat ctttcgtcca taccgaagat gcaagtccgt actggctgtc
     3121 attcgcccag ttcaccacct gttcttcgtt gtcgaagggc gtaacactca ctactggacc
     3181 aaatacctct ttttgcacga tggcatcgtc ctgtaatgcg ccagccagca gcgtcggcgc
     3241 atagtaatag ccattaccct tgcgcttttc accgccagtg atcactttga tgtgccctgt
     3301 cgctttcgcc tcttctactg ccttgctgac gcgttcgaga tgcgccagcg agcttaaagg
     3361 tccaagctcc gtagactcgt catccggcgc accagatttt aacgttgcca ccgcagcacc
     3421 cagtttttcc accagcgtat cgtaaatgcc tttttgcgcg tagatccgac aagccgcagt
     3481 acaatcctgt ccagcattgt aatagccaaa tgtacgtaca ccttcgacca ctgcttcaat
     3541 atccgcatca tcaaaaacaa tcactggcgc tttgccacca agttccatat gagtacgctt
     3601 aatggacgac gcggtatggc tgatgatgtg ctcgccggtg gcgatagagc ccgtcagcga
     3661 caccatccgc actttgggat gaccggtcag cggatcaccc accgttttgc ctctgccaaa
     3721 cagtatgtta atcacgcctg ccgggaagat atctttcgcc agctctgcca acttcaacgc
     3781 ggtcagcggg gtaatttctg atggtttaag cactacgcag ttccctgccg ccagcgccgg
     3841 agcaagtttc cacgcggcca tcatcagcgg ataattccac ggtgcgatag aagccacgac
     3901 ccccaacgga tcgcgacgga tcatcgaagt atgaccttca agatattcac ctgccgccag
     3961 accattcaga cagcgcgccg cacccgcgaa aaagcgaaaa acatcgacaa tcgccgggat
     4021 ttcatcattg aacgcactat gcagcggttt gccacaatta cgggattcca gttcggcaaa
     4081 aacctgacca ttttcttcga taacatccgc cagtttcagc aggcattccg cacgcgcttt
     4141 cggtgtggtt tgcccccatt cagcaaatgc tgcatctgcc gcacgcacag cagcatcgac
     4201 ctgctctgcg gatacctcgg caatctccag taaaacgtcc cccgttgccg gattatagac
     4261 aggctgtttt tccccttcgc cgctaacccg ttctccgtta atcagtaact tatgttgcat
     4321 agcttattcc tgtatcagtg gttattgacc attgtcgcct tcgcgggtta gccaccaggc
     4381 ccccaggatc ggcaaggttg ttaccaacat aaccagcagt gccaccacgt tggttaccgg
     4441 tacatcacgc ggtcgcccaa gctgattgag caaccacaac ggtaacgttc gttcatgacc
     4501 tgccgtaaag gtcgtaacga tgatttcatc gaacgacaag gcaaacgcca gcattcctcc
     4561 tgccagtaac gccgaactga gattcggcaa cactacgtag cggaaggttt gccagccatt
     4621 ggccccaaga tccattgacg cctcaaccag actccacgag gtgcggcgaa aacgggcgat
     4681 gacattgtta aacaccacca ctacgcaaaa agtcgcatga ccgaccacga tggtgaaaaa
     4741 tcccggctcc agattgatgg ttttaaaggc ggttaatagc gccagaccag tgacaatgcc
     4801 cggcagcgca atgggcagca gcaataacag cgaaatggcg tttttgccga aaaagtctcg
     4861 tctccacagc gcggcagctg ccagcgtccc taacaccagc gcaattaatg tcgccagcgc
     4921 cgccacttta agtgacagcg tcacggcatc aagaatatca ctacgctgtg ctgccacgct
     4981 aaaccagcgc agcgtcaggc cctgcggtgg aaaactaaac gccgcatctt cagtgttaaa
     5041 cgcataggcg gcgattatca ggatgggaaa atgtaggaaa acaacgccgc cccaggccgc
     5101 cagtttgagg aaaaacggtg cgcgttcaga gtgcatcgaa cgctcccaga cgtttcacga
     5161 acgccaggta cagtgcgatg agaataatcg gcaccagggt gaatgccgcc gccatcggca
     5221 tattgccaat cgccccctgc tgggaataaa ccatattgcc gataaaatat cctggaggac
     5281 caaccagctg cgggacgata aaatcgccca gtgtgagtga gaaggtaaag atagagccag
     5341 cggcaatacc cgggattgcc agcggcagca ccacatagcg aaaggtttgt cgtggacgtg
     5401 cgccgagatc agccgacgcc tgcaacaatg acggcggcaa acgctcaagc gccgcctgaa
     5461 cgggcaggat
//
LOCUS       DAJTRC010000175         5450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.177, whole genome shotgun sequence.
ACCESSION   DAJTRC010000175 DAJTRC010000000
VERSION     DAJTRC010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.177"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(307..462)
                     /gene="hokD"
                     /locus_tag="OO357_003883"
     CDS             complement(307..462)
                     /gene="hokD"
                     /locus_tag="OO357_003883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokD"
                     /protein_id="HCR5888648.1"
                     /translation="MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVF
                     TAYEPEE"
     gene            complement(1114..1530)
                     /locus_tag="OO357_003884"
     CDS             complement(1114..1530)
                     /locus_tag="OO357_003884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888649.1"
                     /translation="MTELTKEQLIEEAKLKIAITKCHPNSGMARVEGELFKIARASLE
                     AEPIAWRYRYVKKGVMDSQGELWVGDWKYVPKKEDCNDRPNYEIQALFTAPPVPVTSE
                     ELVKAVHFYEQLKRENPPASGNQINGLTMSVKRPAN"
     gene            1557..1697
                     /locus_tag="OO357_003885"
                     /pseudo
     CDS             1557..1697
                     /locus_tag="OO357_003885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310799.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4224 domain-containing protein"
     gene            1697..2722
                     /locus_tag="OO357_003886"
     CDS             1697..2722
                     /locus_tag="OO357_003886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5888650.1"
                     /translation="MGRRRKNPEHEKLPPNVYPNKYSYVWKPTSRESVTLTAIKDGLA
                     ALWKKYEETVNNRDRAMTFGRLWEKFLASAYYSDLSPRTQKDYLQHQKKLLAVFGKVP
                     ADSIKPEHIRRYMDKRGEQSKTQANHEKSSMSRVYSWGYERGYVKANPCAGVSKFKAK
                     NRERYVTDKEYQAVLSVAPLPVFIAMEIAYLCAARVSDVLSLKWEQIGNDGIFIQQGK
                     TGKKQIKAWSPRLQAAIEKAKQLPTSAYVISNQYGNRYMYKGFNEMWVEARNRAGKIS
                     GILTDFTFHDLKAKGISDYEGSSRDKQLFSGHKTEGQVLIYDRKVKVSPTLDVPLPEN
                     IPRKYSK"
     gene            complement(2775..2864)
                     /locus_tag="OO357_003887"
     tRNA            complement(2775..2864)
                     /locus_tag="OO357_003887"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2828..2830),aa:Ser,seq:cga)
     gene            2958..3755
                     /gene="mtfA"
                     /locus_tag="OO357_003888"
     CDS             2958..3755
                     /gene="mtfA"
                     /locus_tag="OO357_003888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310801.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DgsA anti-repressor MtfA"
                     /protein_id="HCR5888651.1"
                     /translation="MIKWPWKVQESAHQTALPWQEALSIPLLTCLTEQEQSKLVALAE
                     RFLQQKRLVPLQGFELNSLRSCRIALLFCLPVLELGLEWLDGFHEVLIYPAPFVVDDE
                     WEDDIGLVHNQRIVQSGQSWQQGPIVLNWLDIQDSFDASGFNLIIHEVAHKLDTRNGD
                     RASGVPFISLREVAGWEHDLHAAMNNIQEEIELVGENAASIDAYAASDPAECFAVLSE
                     YFFSAPELFAPRFPSLWQRFCQFYQQDPLQRLHHANDTDSFSATNVH"
     gene            3856..3931
                     /locus_tag="OO357_003889"
     tRNA            3856..3931
                     /locus_tag="OO357_003889"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3889..3891,aa:Asn,seq:gtt)
     gene            4093..5355
                     /locus_tag="OO357_003890"
                     /pseudo
     CDS             4093..5355
                     /locus_tag="OO357_003890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005227760.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
BASE COUNT         1478 a         1294 c         1250 g         1428 t
ORIGIN      
        1 gaatcagcca ttcagcctgt tcttcggtgc aggggtcgtg ctgatacccg tcagatttga
       61 atgcatgaga acgccgcccg tgcctgctgg caggggcggc agagttatcc gaattgtaaa
      121 atttggtatc gtgcgccatc tgttttctct gctggcgcag caggtgccag ttgttcaggc
      181 tgacggatgg attgtaaacc agaacgacca gaaaaaacaa aacccgccga agcgggttaa
      241 gtgcgggtgc gttgaggatg cctgacacat cagcggtggc gagggatttc tcccccgccg
      301 ggtctcttac tcctcaggtt cgtaagctgt gaagacagcg acctccgtct ggccggttcg
      361 gattcgtacc tcgcagaggt ctttcctcgt taccagtgcc gtcactatga cggttaaaca
      421 gatgacgata agggcgatta acatcgcctt ttgctgcttc atagcctgct tctccttgcc
      481 tttcggcacg taagaggcta acctacatgt gttcagcatg gattgagcct cagattaatg
      541 ttaagcgtct tgcaggacgc gtaatgttaa ctggggcttt tctctatctg ccttttggtg
      601 ttcatgcctg agacagatag cctcaagcac ccgcagtcat tctacttaac taagatttcc
      661 ctgcaaaccg tttttgtccg gcacagtaaa tatccaacta aaccaatagc gttcgctgta
      721 tttaccgcca gtattcaatg cacatgaccg ccatgaacac ccctaaaaaa agggcattta
      781 tatgtccaaa cattaatatc aaaacatcaa ttttttccat ataccttgct gtgaagatga
      841 tgggcataca tgatgcgaac aaccagaacg caacaaacaa aaactgcaat gcgtttttca
      901 ttattccccc tacaatcaat gtgcaataac atttaaacac acctcaattt ggccggacat
      961 ataaatatct aaaccagaaa aaatcactta catagcgtta caaactcttt agtctaaata
     1021 ctcatcgtaa aacattcccc atacttatca gtccgttccg cgccaggtag cttattgcct
     1081 tatctggcaa cctataatca ggtttccgct ttttcagttg gctggtcgtt taaccgacat
     1141 agttaaccca ttaatctggt tgccggatgc tggtggattt tcgcgtttta attgttcata
     1201 aaagtgcaca gctttaacca gttcttctga tgtaaccggg actggtgggg cagtgaataa
     1261 ggcctgaatt tcatagttcg gcctgtcgtt acaatcctct tttttcggta catatttcca
     1321 gtcaccaacc cacaactccc cctgagagtc cataacacct tttttcacgt agcgatatcg
     1381 ccacgctatc ggctctgctt ccagcgatgc ccgtgcaatt ttgaataact cgccctctac
     1441 tcgcgccatc cctgaattgg ggtggcattt cgtaatcgct atttttaatt tggcttcttc
     1501 gattaattgt tcttttgtta attcagtcat tttcattacc gccctggtaa tgctgccacc
     1561 agcgaaaatg gttaatggaa aacgggcttc cgttctatac caatcgtagt ggcaaaccga
     1621 ttgtcagccg ggatctattt acctgcaata aaactttacc accacgcgag gtagagccga
     1681 attttggtgc gatctgatgg gaagacgaag gaaaaatcct gaacacgaaa aattacctcc
     1741 aaatgtatac ccaaataaat atagttatgt atggaaacca acatccagag aatctgtaac
     1801 actaaccgcc atcaaggatg gtttagctgc tttatggaaa aagtatgagg aaactgtaaa
     1861 taatcgcgat cgtgcaatga cattcggtcg cttgtgggaa aaattcctcg ccagcgccta
     1921 ttacagtgac ctcagtccaa gaacacaaaa agattatctg caacatcaaa aaaagttgct
     1981 tgccgtattc ggtaaggtgc cggcagattc cataaaacca gaacacatcc gtcgatacat
     2041 ggacaagaga ggggaacaga gtaaaacgca agccaaccat gaaaaaagca gtatgtcccg
     2101 cgtttacagt tgggggtatg agcgagggta cgtgaaggct aacccatgtg caggtgtaag
     2161 taaattcaag gccaaaaacc gcgaacgata tgtaaccgac aaagaatacc aggcagtatt
     2221 aagcgttgca cctcttcctg tttttatcgc aatggaaatt gcctatctgt gtgcagcgag
     2281 ggtttccgat gtgttatcgc tgaaatggga gcagattgga aacgacggga tctttatcca
     2341 gcaagggaaa acaggaaaaa aacagataaa agcatggagt ccacgattac aggcggcgat
     2401 cgaaaaagca aaacagttac caacatccgc ctatgtaatc agcaatcaat acggcaaccg
     2461 atatatgtac aaaggcttta acgaaatgtg ggtagaagca agaaatcgcg caggcaaaat
     2521 ttcaggtatt ttaaccgact tcacctttca tgatctgaag gcgaaaggaa tttcagacta
     2581 tgaaggaagc agtcgggata agcaactttt ctctggtcac aaaaccgagg ggcaagtgct
     2641 aatctatgac aggaaggtta aagtttcacc gacacttgat gtcccgttac ctgaaaatat
     2701 tccaagaaaa tattccaagt aattccaagt gtgatttttg tcactgactt aatgatgtat
     2761 aagtgattga attttggcgg agagaggggg atttgaaccc ccggtggagt tgcccccact
     2821 ccggttttcg agaccggtcc gttcagccgc tccggcatct ctccgttcag atggttgcca
     2881 tgatgccagg aaatttggca ttttaacagt ccctgtccgt gcaattttgt tcaagtgacg
     2941 agtttgcgag caaaacgatg attaagtggc cctggaaagt acaagaatca gcacatcaaa
     3001 ctgcccttcc ctggcaggaa gcactatcga tccccctttt aacgtgtctg acagaacagg
     3061 aacaaagcaa attggtcgct cttgccgaac gttttttaca gcaaaaacgg cttgttcctt
     3121 tacagggctt tgagctgaat tcattaagaa gctgccggat agcacttcta ttttgcctgc
     3181 ccgttctgga gttaggactg gaatggctgg atggttttca tgaagtctta atttatcctg
     3241 cgccatttgt ggtcgatgat gaatgggaag acgatatcgg tctggtgcat aaccaacgta
     3301 ttgttcagtc aggtcagagc tggcagcaag ggcctatcgt tttgaactgg ttggatatac
     3361 aagattcttt tgatgcttct ggttttaacc tgattattca tgaagtcgct cataagctgg
     3421 acacccgtaa cggcgatcgc gccagcggag ttccctttat ttcgttgcgt gaggttgctg
     3481 gctgggaaca cgatcttcat gctgcaatga acaacattca ggaagaaatc gaattagttg
     3541 gtgagaatgc ggcgagcatt gatgcttatg ctgccagtga tcctgctgaa tgttttgccg
     3601 tactttctga atatttcttt agcgccccag aactttttgc tcctcgtttc ccttcattgt
     3661 ggcaacgttt ctgtcaattt tatcaacaag atcctttgca gagactgcat cacgctaatg
     3721 atacagactc gttttcagcg acgaatgttc attaattaac aactttgaag attaattaac
     3781 caattgaaat ggcttatgaa atttagtgtt gacagacaag gtaccgctaa gtaatatgcg
     3841 ccccgttctc acgattcctc tgtagttcag tcggtagaac ggcggactgt taatccgtat
     3901 gtcactggtt cgagtccagt cagaggagcc aattttctgt tttcatgcat ccttgcgaat
     3961 ctttatctga tgttgattca acaggttagt gtgaaaactc ttctcggtgc attttgattt
     4021 taccctacgc atcgggaaaa attggtggtc aaatctggga tcaggttagt tcgataatgg
     4081 agtgaccccc atatatccct taccgacgca aaaatccgca ccctcaagcc ttctgataaa
     4141 ccctttaaag tctccgattc tcacggtctg tatctgctgg tcaagccggg tggctcccgc
     4201 cactggtatc tcaaataccg tattagcggt aaagaatccc gcattgcgct gggtgcctat
     4261 ccagccatct ccctgtctga tgcgcgacag caacgtgaag gtatccgtaa aatgctggcg
     4321 ctgaatatca acccggtaca gcagcgggct gctgaacgtg gctcacgaac accggagaaa
     4381 gtttttaaaa acatggcgct ggcgtgacat aaaagtaaca ggaaatggtc gcagaacacc
     4441 gccgaccgtc tgcttgccag cctgaacaat cacatctttc cggtcatcgg gaacctacct
     4501 gtatcagaac ttaaaccccg tcatttcatt gacctgctga aagggatcga ggaaaaaggt
     4561 ctgctggagg ttgcgtcccg cacacggcag cacctgagta acataatgcg ccatgcggtc
     4621 catcaggagt taatcgatac gaaccctgca gcaaaccttg gcggcgtgac cacacctcct
     4681 gtcagacggc actatcctgc cctgccgctg gagcggctgc ctgaactgct tgaacgtatt
     4741 ggggcatatc atcagggccg tgaactgacc cggcatgccg ttctgctgat gctgcatgtg
     4801 ttcattcgct ccagtgaact gcgtttcgcc cgctggtcag agattgattt cacaaaccga
     4861 gtctggacga tacccgcgac gcgagaaccc attattggcg tgcgttattc cggccgcggg
     4921 gcaaaaatgc gaatgccgca tatcgtcccc ctctcagaac agtccatcgc cattctgaaa
     4981 cagattaagg atatcaccgg taataatgaa ctgatcttcc ccggcgacca taacccgtat
     5041 aagccaatgt gtgaaaacac ggtcaataag gcactgcggg tgatgggtta cgacacgaaa
     5101 aaggatatct gcggtcacgg cttccgggca atggcatgca gtgcgctgat ggaatcgggt
     5161 ttatgggcaa aggacgcagt agaacgccag atgagtcatc aggagcgcaa taccgtgcgc
     5221 atggcttata ttcataaggc agagcacctg gaagcccgca aagcgatgat gcagtggtgg
     5281 tcggattatc tggaagcatg ccgagaatct tatgcaccgc cttatacaat tggtaaaaat
     5341 aagtttatcc catagtatac taacgaatcc cacactctgg caaattacca ccacctctac
     5401 aggaggtggt tctatataga cttttatcaa cttcatgacg aaaaacacct
//
LOCUS       DAJTRC010000176         5390 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.178, whole genome shotgun sequence.
ACCESSION   DAJTRC010000176 DAJTRC010000000
VERSION     DAJTRC010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5390
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.178"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            1..487
                     /locus_tag="OO357_003891"
                     /pseudo
     CDS             1..487
                     /locus_tag="OO357_003891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414966.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="COX aromatic rich motif-containing protein"
     gene            509..2500
                     /gene="cyoB"
                     /locus_tag="OO357_003892"
                     /pseudo
     CDS             509..2500
                     /gene="cyoB"
                     /locus_tag="OO357_003892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002444673.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit I"
     gene            2490..3104
                     /locus_tag="OO357_003893"
     CDS             2490..3104
                     /locus_tag="OO357_003893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit III"
                     /protein_id="HCR5888652.1"
                     /translation="MATDTLTHATAHAHEHGHHDAGGTKIFGFWIYLMSDCILFSILF
                     ATYAVLVNGTAGGPTGKDIFELPFVLVETFLLLFSSITYGMAAIAMYKNNKSQVISWL
                     ALTWLFGAGFIGMEIYEFHHLIVNGMGPDRSGFLSAFFALVGTHGLHVTSGLIWMAVL
                     MVQIARRGLTSTNRTRIMCLSLFWHFLDVVWICVFTVVYLMGAM"
     gene            3104..3433
                     /locus_tag="OO357_003894"
     CDS             3104..3433
                     /locus_tag="OO357_003894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome o ubiquinol oxidase subunit IV"
                     /protein_id="HCR5888653.1"
                     /translation="MSHSTDHSGASHGSVKTYMTGFILSIILTVIPFWMVMTGAASPA
                     VILGTILAMAVVQVLVHLVCFLHMNTKSDEGWNMTAFVFTVLIIAILVVGSIWIMWNL
                     NYNMMMH"
     gene            3445..4335
                     /gene="cyoE"
                     /locus_tag="OO357_003895"
     CDS             3445..4335
                     /gene="cyoE"
                     /locus_tag="OO357_003895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protoheme IX farnesyltransferase"
                     /protein_id="HCR5888654.1"
                     /translation="MMFKQYLQVTKPGIIFGNLISVIGGFLLASKGSIDYPLFIYTLV
                     GVSLVVASGCVFNNYIDRDIDRKMERTKNRVLVKGLISPAVSLVYATLLGIAGFMLLW
                     FGANPLACWLGVMGFVVYVGVYSLYMKRHSVYGTLIGSLSGAAPSVIGYCAVTGEFDS
                     GAAILLAIFSLWQMPHSYAIAIFRFKDYQAANIPVLPVVKGISVAKNHITLYIIAFAV
                     ATLMLSLGGYAGYKYLVVAAAVSVWWLGMALRGYKVADDRIWARKLFGFSIIAITALS
                     VMMSVDFMVPDSHTLLAAVW"
     gene            4519..4830
                     /locus_tag="OO357_003896"
     CDS             4519..4830
                     /locus_tag="OO357_003896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5888655.1"
                     /translation="MPGKTAALYDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLS
                     AFILSAAMERAESVLDNQRRRELSNQSWELMNQLIAEPAQPTLALKALMKRKNSDGRQ
                     A"
     gene            4965..5363
                     /locus_tag="OO357_003897"
     CDS             4965..5363
                     /locus_tag="OO357_003897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5888656.1"
                     /translation="MRNSDCAAKALIDRQSGELIGICTFTAYSLERQRVSGVLQGSQP
                     SEIGVVRLVMLGGARKYQKRGFGQDLLCDFFEHVKKIHPALPIKGVYLDADPAAINFY
                     ARLGFVQLSATPNAFGAVPMFLAIQHILAA"
BASE COUNT         1174 a         1341 c         1424 g         1451 t
ORIGIN      
        1 tttcccggcg aacactccgg tgtacttcaa agtgacctcc aactccgtga tgaactcctt
       61 cttcattccg cgtctgggta gccagattta tgccatggcc ggtatgcaga ctcgcctgca
      121 tctgatcgcc aacgaacccg gtacttatga cggtatctcc gccagctaca gcgggccggg
      181 cttctcaggc atgaagttca aagctattgc aacaccggat cgcgccgaat tcgaccaatg
      241 ggtcgcaaaa gcgaaacagt cgccgaacac catgtctgac atggctgcgt tcgaaaaact
      301 ggccgcgcct agcgaataca actaggtgga atatttctcc aacgtgaaac cagacttgtt
      361 tgccgatgtg attaacaagt ttatggctca cggtaagagc atggacatga cccagccaga
      421 aggtgagcac agtgcacacg aaggtatgga aggcatggac atgagccacg cggaatccgc
      481 ccattaaagg ggttgaggaa gaataaagat gttcggaaaa ttatcacttg atgcagtccc
      541 gttccatgaa cctatcgtca tggttacgat cgctggcatt attttgggag gtctggcgct
      601 cgttggcctg atcacttact tcggtaagtg gacctacctg tggaaagagt ggctgacctc
      661 cgtcgaccat aaacgcctcg gtatcatgta tatcatcgtg gcgattgtga tgttgctgcg
      721 tggttttgct gacgccatta tgatgcgtag ccagcaggct cttgcctcgg cgggtgaagc
      781 gggcttcctg ccacctcacc actacgatca gatcttcacc gcgcacggcg tgattatgat
      841 cttcttcgtg gcgatgcctt tcgttatcgg tctgatgaac ctggtggttc cgctgcagat
      901 cggcgcgcgt gacgttgcgt tcccgttcct caacaactta agcttctggt ttactgttgt
      961 tggcgtgatt ctggttaacg tttctctcgg cgtgggcgaa tttgcgcaga ccggctggct
     1021 ggcctatcca ccgctatcgg gaatagagta cagtccggga gtcggtgttg attactggat
     1081 atggagtctc cagctatccg gtataggtac gacgcttacc ggtatcaact tcttcgttac
     1141 cattctgaag atgcgcgcac cgggcatgac catgttcaag ataccagtat ttacctaggc
     1201 atcactgtgc gcgaacgtac tgattattgc ttccttccca attctgacgg ttaccgtcgc
     1261 gttgttgacc ctggatcgct atctgggcac ccatttcttt accaacgata tgggtggcaa
     1321 catgatgatg tacatcaacc tgatttgggc ctggggccac ccggaagttt acatcctgat
     1381 cctgcctgtt ttcggtgtgt tctccgaaat tgcggcaacc ttctcgcgta aacgtctgtt
     1441 tggttatacc tcgctggtat gggcaaccgt ctgcatcacc gtgctgtcgt tcatcgtttg
     1501 gctgcaccac ttctttacga tgggtgcggg cgcgaacgta aacgccttct ttggtatcac
     1561 cacaatgatt atcgccatcc cgaccggggt gaagatcttc aactggctgt tcaccatgta
     1621 tcagggccgc atcgtgttcc attctgcgat gctgtggact atcggtttta tcgtcacctt
     1681 ctcggtgggc ggtatgactg gcgtgctgct ggcagtaccg ggcgcggact tcgttctgca
     1741 taacagcctg ttcctgattg cgcacttcca taacgtgatc atcggcggcg tggtcttcgg
     1801 ctgcttcgca gggatgacct actggtggcc taaagcgttc ggtttcaaac tgaacgaaac
     1861 ctggggtaaa cgcgcgttct ggttctggat catcggcttc ttcgttgcct ttatgccgct
     1921 gtatgcgctg ggcttcatgg gcatgacccg tcgtttgagc cagcagattg acccgcagtt
     1981 ccacaccatg ctgatgattg cagccagcgg tgcagtactg attgcgctgg gtattctctg
     2041 cctcgttatt cagatgtacg tttctattcg cgaccgcgac cagaaccgtg acctgactgg
     2101 cgacccgtgg ggtggccgta cgctggagtg ggcaacctct tccccgcctc cgttctataa
     2161 ctttgccgta gtgccgcacg ttcacgaacg tgatgcattc tgggaaatga aagagaaagg
     2221 cgaagcgtat aaaaagcctg accactatga agaaattcat atgccgaaaa acagcggtgc
     2281 aggtatcgtc attgcagctt tctccaccat cttcggtttc gccatgatct ggcatatctg
     2341 gtggctggcg attgttggct tcgcaggcat gatcatcacc tggatcgtga aaagcttcga
     2401 cgaggacgtg gattactacg tgccggtggc agaaatcgaa aaactggaaa accagcattt
     2461 cgatgagatt actaaggcag ggctgaaaaa tggcaactga tactttgacg cacgcgactg
     2521 cccacgcgca cgaacacggg caccacgatg caggcggaac caaaatcttc ggattttgga
     2581 tctacctgat gagcgactgc attctgttct caatcttgtt tgctacctat gccgttctgg
     2641 tgaacggcac cgcaggcggc ccgacaggta aggacatttt cgaactgccg ttcgttctgg
     2701 ttgaaacttt cttgctgttg ttcagctcca tcacctacgg catggcggct atcgccatgt
     2761 acaaaaacaa caaaagccag gttatctcct ggctggcgtt gacctggttg tttggtgccg
     2821 gatttatcgg gatggaaatc tatgaattcc atcacctgat tgttaacggc atggggccgg
     2881 atcgcagcgg cttcctgtca gcgttctttg cgctggtcgg cacgcacggt ctgcacgtca
     2941 cttctggtct tatctggatg gcggtgctga tggtgcaaat cgcccgtcgc ggcctgacca
     3001 gcactaaccg tacccgcatc atgtgcctga gcctgttctg gcacttcctg gatgtggttt
     3061 ggatctgtgt gttcactgtt gtttatctga tgggggcgat gtaatgagtc attctaccga
     3121 tcacagcggc gcgtcccatg gcagcgtaaa aacctacatg acaggcttta tcctgtcgat
     3181 cattctgacg gtgattccgt tctggatggt gatgacaggg gctgcctctc cggccgtaat
     3241 tctgggaaca atcctggcaa tggcagtggt acaggttctg gtgcatctgg tgtgcttcct
     3301 gcacatgaat accaaatcag atgaaggctg gaacatgacg gcgtttgtct tcaccgtgct
     3361 aatcatcgct atcctggttg taggctccat ctggattatg tggaacctca actacaacat
     3421 gatgatgcac taagagcggc ggttatgatg tttaagcaat acctgcaagt aacgaaacca
     3481 ggcatcatct ttggcaacct gatctcggtg attgggggat tcctgctggc ctcaaagggc
     3541 agcattgatt atcccctgtt tatctacacg ctggttgggg tgtcactggt tgtggcgtcg
     3601 ggttgtgtgt ttaacaacta catcgacagg gatatcgaca gaaagatgga aaggacgaag
     3661 aatcgggtgc tggttaaagg cctgatctct cctgctgtct cgctggtgta cgccaccttg
     3721 ctgggtattg ctggctttat gctgctgtgg tttggcgcga atccgctggc ctgctggctg
     3781 ggggtgatgg gctttgtggt ttatgtcggc gtctatagcc tgtacatgaa acgccactca
     3841 gtctacggca cattgattgg ttcgctctcc ggcgctgcgc cgtcggtgat tggctactgt
     3901 gcagtaaccg gcgagtttga tagcggcgca gcgatcctgc tggctatctt cagcctgtgg
     3961 cagatgcctc actcctatgc catcgccatt ttccgcttta aggattacca ggcggcaaac
     4021 attccggtat tgccagtggt aaaaggcatt tcggtggcga agaatcacat cacgctgtat
     4081 atcatcgcct ttgccgttgc cacgctgatg ctctctcttg gcggttacgc tgggtataaa
     4141 tatctggtgg tcgccgcggc ggttagcgtc tggtggttag gtatggctct gcgcggttat
     4201 aaagttgctg atgacagaat ctgggcgcgc aagctgttcg gcttctctat catcgccatc
     4261 actgccctct cggtgatgat gtccgttgat tttatggtac cggactcgca tacgctgctg
     4321 gctgctgtgt ggtaacaaaa cctctctatt aaaaaggtgc tacggcacct ttttattgtc
     4381 tgagatcaga gatacggcta ataaccgtat ctcttgacag agtgagcaga tgcacataaa
     4441 ttaatcatac ggttgatagc cgtattatgt atgagatcga ctccctacga tttcattcag
     4501 ttaatcaaag aagtcataat gcctggaaaa acagccgctc tatacgatgt cgataaaacg
     4561 ctgaaaaatg cgcgggtcga actcaaaact agtcccgacg ccaaaaacaa acttcgtgaa
     4621 gctgcgcaag ccgttggggt agatttaagc gcttttattt tgagcgctgc tatggaacgc
     4681 gccgaaagtg tgcttgataa ccaacgtcgc cgtgagcttt cgaatcaaag ctgggaactg
     4741 atgaaccaac tcatcgctga acctgctcaa ccgacgctcg ccctcaaggc gttaatgaaa
     4801 aggaaaaaca gcgatggtcg acaagcatga agagattact ctgcccatag tcctctcctg
     4861 taattatcag tctgatatta cttatcctgg gcaaaaacag tttgattgcg gtaaccctgt
     4921 tatcgataaa tttgtacgcg ctacgcgcat cgctaaagaa aagtgtgcgt aatagcgact
     4981 gtgcggctaa agcacttatt gacagacaaa gtggtgaact gattggcatc tgtactttta
     5041 cggcatattc gctggaaaga caacgcgttt ctggcgtcct tcagggttca caaccttcag
     5101 aaattggtgt tgtcagatta gtcatgttgg ggggagcgcg gaagtatcaa aagcggggct
     5161 ttggtcagga cctactatgt gatttttttg aacatgtaaa aaaaattcac ccggcattac
     5221 caattaaagg ggtttatctt gatgctgacc ctgccgccat taacttttat gctcgtctcg
     5281 gctttgttca gctttcagcg acaccaaatg cttttggtgc tgtacctatg tttttggcga
     5341 ttcagcatat tctcgcggct tagattatta tcgccacccg ccagggcaag
//
LOCUS       DAJTRC010000177         5327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.179, whole genome shotgun sequence.
ACCESSION   DAJTRC010000177 DAJTRC010000000
VERSION     DAJTRC010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.179"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(23..760)
                     /gene="bamD"
                     /locus_tag="OO357_003898"
     CDS             complement(23..760)
                     /gene="bamD"
                     /locus_tag="OO357_003898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HCR5888657.1"
                     /translation="MTRMKYLVAAATLSLFLAGCSGSKEEVPDNPPNEIYATAQQKLQ
                     DGNWRQAITQLEALDNRYPFGPYSQQVQLDLIYAYYKNADLPLAQAAIDRFIRLNPTH
                     PNIDYVMYMRGLTNMALDDSALQGFFGVDRSDRDPQHARAAFSDFSKLVRGYPNSQYT
                     TDATKRLVFLKDRLAKYEYSVAEYYTERGAWVAVVNRIEGMLRDYPDTQATRDALPLM
                     ENAYRQMQMNAQAEKVAKIIAANSSNT"
     gene            895..1875
                     /gene="rluD"
                     /locus_tag="OO357_003899"
     CDS             895..1875
                     /gene="rluD"
                     /locus_tag="OO357_003899"
                     /EC_number="5.4.99.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017383977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA pseudouridine(1911/1915/1917) synthase
                     RluD"
                     /protein_id="HCR5888658.1"
                     /translation="MAQRVQLTATVSENQLGQRLDQALAEMFPDYSRSRIKEWILDQR
                     VLVNGKVCDKPKEKVLGGEQVAINAEIEEEARFEPQDIPLDIVYEDEDIIVINKPRDL
                     VVHPGAGNPDGTVLNALLHYYPPIADVPRAGIVHRLDKDTTGLMVVAKTVPAQTRLVE
                     SLQRREITREYEAVAIGHMTAGGTVDEPISRHPTKRTHMAVHPMGKPAVTHYRIMEHF
                     RVHTRLRLRLETGRTHQIRVHMAHITHPLVGDPVYGGRPRPPKGASEAFISTLRKFDR
                     QALHATMLRLYHPISGIEMEWHAPIPQDMVELIEVMRADFEEHKDEVDWL"
     gene            1872..2603
                     /gene="pgeF"
                     /locus_tag="OO357_003900"
     CDS             1872..2603
                     /gene="pgeF"
                     /locus_tag="OO357_003900"
                     /EC_number="1.10.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase"
                     /protein_id="HCR5888659.1"
                     /translation="MSKLIVPQWPLPKGVAACSSTRIGGVSLPPYDSLNLGAHCGDNP
                     DHVEENRKRLFAAGNLPSKPVWLEQVHGKDVLKLTGEPYASKRADASYSNTPGTVCAV
                     MTADCLPVLFCNRAGTEVAAVHAGWRGLCAGVLEETVSCFADKPENILAWLGPAIGPR
                     AFEVGAEVREAFMAVDAKASAAFIQHGDKYLADIYQLARQRLANVGVEQIFGGDRCTY
                     TENETFFSYRRDKTTGRMASFIWLI"
     gene            2733..5306
                     /gene="clpB"
                     /locus_tag="OO357_003901"
     CDS             2733..5306
                     /gene="clpB"
                     /locus_tag="OO357_003901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002461515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HCR5888660.1"
                     /translation="MRLDRLTNKFQLALADAQSLALGHDNQFIEPLHLMSALLNQEGG
                     SVSPLLTSAGINAGQLRTDINQALNRLPQVEGTGGDVQPSQDLVRVLNLCDKLAQKRG
                     DNFISSELFVLAALESRGTLADILKATGATTANITQAIEQMRGGESVNDQGAEDQRQA
                     LKKYTIDLTERAEQGKLDPVIGRDEEIRRTIQVLQRRTKNNPVLIGEPGVGKTAIVEG
                     LAQRIINGEVPEGLKGRRVLALDMGALVAGAKYRGEFEERLKGVLNDLAKQEGNVILF
                     IDELHTMVGAGKADGAMDAGNMLKPALARGELHCVGATTLDEYRQYIEKDAALERRFQ
                     KVFVAEPSVEDTIAILRGLKERYELHHHVQITDPAIVAAATLSHRYIADRQLPDKAID
                     LIDEAASSIRMQIDSKPEELDRLDRRIIQLKLEQQALMKESDEASKKRLDMLNEELSD
                     KERQYSELEEEWKAEKASLSGTQTIKAELEQAKIAIEQARRVGDLARMSELQYGKIPE
                     LEKQLEAATQLEGKTMRLLRNKVTDAEIAEVLARWTGIPVSRMMESEREKLLRMEQEL
                     HHRVIGQNEAVDAVSNAIRRSRAGLADPNRPIGSFLFLGPTGVGKTELCKALANFMFD
                     SDEAMVRIDMSEFMEKHSVSRLVGAPPGYVGYEEGGYLTEAVRRRPYSVILLDEVEKA
                     HPDVFNILLQVLDDGRLTDGQGRTVDFRNTVVIMTSNLGSDLIQERFGELDYAHMKEL
                     VLGVVSHNFRPEFINRIDEVVVFHPLGEQHIASIAQIQLKRLYKRLEERGYEIHISDE
                     ALKLLSENGYDPVYGARPLKRAIQQQIENPLAQQILSGELVPGKVIRLEVNEDRIVAV
                     Q"
BASE COUNT         1308 a         1286 c         1453 g         1280 t
ORIGIN      
        1 cgttttgtgt ttcaggtttc tgttatgtat tgctgctgtt tgcggcgatg attttcgcta
       61 ctttttcagc ttgcgcattc atctgcatct gacggtatgc attttccatc agcggcagcg
      121 catcacgcgt agcctgggta tccgggtagt cgcgcaacat gccttctatg cggttaacga
      181 cggcaaccca tgcgccacgt tctgtatagt actcggccac ggagtattca tatttcgcca
      241 gacgatcttt caggaatacc agacgtttgg tggcatcggt agtgtactga ctgttcggat
      301 agccgcgcac cagtttggaa aagtcactaa acgcagctcg tgcatgttga ggatcgcgat
      361 cgctacggtc aacgccaaag aacccttgca gcgcactgtc atccagcgcc atattggtca
      421 ggccacgcat gtacatgaca taatcgatat tcggatgggt cgggttaagg cgaataaaac
      481 gatcgatggc agcctgcgct aacggcaaat cggcgttttt atagtaggcg tagatgagat
      541 ccagctgcac ctgctgcgaa tacggaccaa acggatagcg attatctaac gcttccagtt
      601 gcgttattgc ctgtctccag ttaccgtcct gcagcttttg ttgtgcagtc gcgtaaattt
      661 catttggcgg attatcaggt acttcttcct ttgaccccga gcaacccgcc aaaaacaggc
      721 ttagtgtggc ggctgccacc agatatttca tgcgcgtcat gacgttttga ctttcctcaa
      781 aatgtaatac gggagattct ctgttcctgc acccggttaa gaccagctac aatagcacac
      841 tatattaaac ggcaaagccg taaaacccca acgataaacg aagaagcagt atatatggca
      901 caacgagtac agctcactgc aacggtgtcc gaaaaccaac tcggtcaacg cttagatcag
      961 gctttggccg aaatgttccc ggattattca cgttcgcgaa taaaagaatg gatcctcgac
     1021 cagcgcgtgc tggtaaacgg caaagtttgt gataagccga aagaaaaagt attgggtggc
     1081 gagcaggttg ccatcaacgc tgagattgaa gaagaagcgc gttttgaacc gcaggatatc
     1141 cctctggata tcgtctatga agatgaagat atcatcgtca ttaataaacc gcgcgaccta
     1201 gtggtacatc ctggcgcggg taacccggat ggcacggtac tgaatgcgtt gcttcattac
     1261 tatccgccca ttgccgatgt accacgtgcg ggcatcgtcc atcgtctgga taaagacacc
     1321 actggcctga tggttgtggc aaaaaccgtt ccggctcaga cgcgtttagt cgaatctttg
     1381 caacggcgtg aaattactcg tgagtatgaa gcggtggcga ttggtcatat gaccgctggc
     1441 ggtacagtag atgaacccat cagccgccac ccgaccaaac gtactcacat ggcggtgcat
     1501 ccgatgggca aaccggcggt gactcactat cgcatcatgg aacacttccg tgtgcacacg
     1561 cgtctgcggt tgcgtctgga aactggacgt acgcaccaga tccgcgtgca tatggcccat
     1621 atcactcatc cgctggtggg cgatccggtt tatggtggcc gtccgcgtcc gccaaaaggt
     1681 gcttcggaag catttatctc cacgctgcgt aagtttgacc gccaggcgct tcatgcaacc
     1741 atgctgcgtc tttatcaccc gatctccggc atcgaaatgg aatggcatgc gcctattcca
     1801 caagatatgg tggagctgat tgaggtgatg cgcgccgatt tcgaagagca taaggatgaa
     1861 gtggactggt tatgagtaag ctgattgtcc cgcagtggcc gctgccaaaa ggtgttgcgg
     1921 cctgtagctc cactcgtatc ggcggcgtga gcttgccccc gtatgactca ctcaacctcg
     1981 gtgcccattg tggcgataac ccggatcacg ttgaggagaa tcgcaagcga ctttttgctg
     2041 cgggcaattt gccttctaaa ccggtctggc ttgagcaggt acacggcaaa gatgtgctta
     2101 agcttactgg cgaaccttat gcctcaaaac gagcagatgc ctcttatagc aatacgcctg
     2161 gcacggtttg cgcagtgatg actgccgact gcctccctgt gctgttttgc aatcgagcgg
     2221 gaacggaagt cgccgccgtt catgctggct ggcgtggact gtgcgcaggc gtgctggaag
     2281 agacggtttc ctgttttgct gataaacctg aaaacattct cgcctggtta gggccggcaa
     2341 ttggtccacg cgcgttcgaa gtgggggcgg aggttcgcga ggcgtttatg gcagtagacg
     2401 ctaaagcaag tgcagctttc attcagcatg gtgataagta tctggcggat atttatcagc
     2461 ttgcccggca gcgtctggcg aacgtgggtg ttgagcaaat tttcggcggc gaccgttgta
     2521 catatacgga aaatgagact ttcttctctt atcgtcgcga caagaccacc ggtcgtatgg
     2581 caagtttcat ttggctgata taacctaaag aatcaagacg atccggtacg cgtgattttc
     2641 ttttcacatt aatctggtca ataaccttga ataattgagg gatgacctca tttaatctcc
     2701 agtagcaact ttgatccgtt atgggaggag ttatgcgtct ggatcgtctt actaataaat
     2761 tccagcttgc tcttgccgat gcccaatcac ttgcactcgg gcacgacaac caattcatcg
     2821 aaccacttca tttaatgagc gccctgctga atcaggaagg gggttcggtt agtcctttat
     2881 taacatccgc tggcataaat gctggccagt tgcgcacaga tatcaatcag gcattaaatc
     2941 gtttaccgca ggttgaaggt actggtggtg atgtccagcc atcacaggat ctggtgcgcg
     3001 ttcttaatct ttgcgacaag ctggcgcaaa aacgtggtga taactttatc tcgtcagaac
     3061 tgttcgttct ggcggcactt gagtctcgcg gcacgctggc cgacatcctg aaagcaacag
     3121 gggcgaccac cgccaacatt actcaagcga ttgaacaaat gcgtggaggt gaaagcgtga
     3181 acgatcaagg tgctgaagac caacgtcagg ctttgaaaaa atataccatc gaccttaccg
     3241 aacgagccga acagggcaaa ctcgatccgg tgattggtcg tgatgaagaa attcgccgta
     3301 ccattcaggt gctgcaacgt cgtactaaaa ataacccggt actgattggt gaacccggcg
     3361 tcggtaaaac tgccatcgtt gaaggtctgg cgcagcgtat tatcaacggc gaagtgccgg
     3421 aaggattgaa aggccgccgc gtactggcgc tggatatggg cgcgctggtg gctggggcga
     3481 aatatcgcgg tgagtttgaa gaacgtttaa aaggcgtgct taacgatctt gccaaacagg
     3541 aaggcaacgt catcctgttt atcgacgaat tacataccat ggtcggcgcg ggtaaagccg
     3601 atggcgcaat ggacgccgga aacatgctga aaccggcgct ggcgcgtggt gaattgcact
     3661 gcgtaggtgc cacgacgctt gacgaatatc gccagtacat cgaaaaagat gctgcgctgg
     3721 aacgtcgttt ccagaaagtg tttgttgccg agccttctgt tgaagatacc attgcgattc
     3781 tgcgtggcct gaaagaacgt tacgaattgc accaccatgt gcaaattact gacccggcaa
     3841 ttgttgcagc agcgacgttg tctcatcgct acattgctga ccgtcagctg ccggataaag
     3901 ccatcgacct gatcgatgaa gcagcatcca gcattcgtat gcagattgac tcaaaaccag
     3961 aagaactcga ccgactcgat cgtcgtatca tccagctcaa actggaacaa caggcgttaa
     4021 tgaaagagtc tgatgaagcc agtaaaaaac gcctggatat gctcaacgaa gaactgagcg
     4081 acaaagaacg tcagtactcc gagttagaag aagagtggaa agcagagaag gcatcgcttt
     4141 ctggtacgca gaccattaaa gcggaactgg aacaggcgaa aatcgctatt gaacaggctc
     4201 gccgtgtggg ggacctggcg cggatgtctg aactgcaata cggcaaaatc ccggaactgg
     4261 aaaagcaact ggaagccgca acgcagctcg aaggcaaaac tatgcgtctg ttgcgtaata
     4321 aagtgaccga tgccgaaatt gctgaagtgc tggcgcgttg gacggggatt ccggtttctc
     4381 gcatgatgga aagcgagcgc gaaaaactgc tgcgtatgga gcaagaactg caccatcgcg
     4441 tcattggtca gaacgaagcg gttgatgcgg tatctaacgc tattcgccgt agccgtgcgg
     4501 ggttggcgga tccaaatcgc ccgattggtt cattcctgtt cctcggccca acgggtgtgg
     4561 ggaaaactga gctttgtaag gcgctggcga actttatgtt tgatagcgac gaggcgatgg
     4621 tccgtatcga tatgtccgaa tttatggaga aacactcggt gtctcgtctg gttggtgcgc
     4681 ctccgggata tgtcggttat gaagaaggtg gctacctgac cgaagctgtg cgtcgtcgtc
     4741 cgtattccgt catcctgctg gatgaagtgg aaaaagcgca tccggatgtc ttcaatattc
     4801 tgttgcaggt actggatgat gggcgtctga ctgacgggca ggggagaacg gtcgacttcc
     4861 gtaatacggt tgtcattatg acctctaacc tcggttccga tctgattcag gaacgcttcg
     4921 gtgaactgga ttatgcgcac atgaaagagc tggtgctcgg tgtggttagc cataacttcc
     4981 gtccggaatt cattaaccgt attgatgaag tggtggtctt ccatccgctg ggtgaacagc
     5041 acattgcctc gattgcgcag attcagttga aacgtctgta caaacgtctg gaagaacgtg
     5101 gttatgaaat ccacatttct gacgaggcgc tgaaactgct gagcgagaac ggttacgatc
     5161 cagtctatgg tgcacgtcct ttgaaacgtg ctatccagca gcagatcgaa aacccgctgg
     5221 cacagcaaat actgtctggt gaattggttc cgggtaaagt gattcgcctg gaagttaatg
     5281 aagaccggat tgtcgctgtc cagtaatgat aaaacgagcc cttcggg
//
LOCUS       DAJTRC010000178         5295 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.180, whole genome shotgun sequence.
ACCESSION   DAJTRC010000178 DAJTRC010000000
VERSION     DAJTRC010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5295
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.180"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..170)
                     /locus_tag="OO357_003902"
     CDS             complement(<1..170)
                     /locus_tag="OO357_003902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888661.1"
                     /translation="MIPELIISGEEANGEFLMLVPGKMLFCSFCVMLMKQVRKIVSIG
                     EPFFFRVIVPFK"
     gene            246..2807
                     /gene="mutS"
                     /locus_tag="OO357_003903"
     CDS             246..2807
                     /gene="mutS"
                     /locus_tag="OO357_003903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HCR5888662.1"
                     /translation="MSAIENFDAHTPMMQQYLKLKAQHPEILLFYRMGDFYELFYDDA
                     KRASQLLDISLTKRGASAGEPIPMAGIPYHAVENYLAKLVNQGESVAICEQIGDPATS
                     KGPVERKVVRIVTPGTISDEALLQERQDNLLAAIWQDSKGFGYATLDISSGRFRLSEP
                     ADRETMAAELQRSNPAELLYAEDFAEMSLIEGRRGLRRRPLWEFEIDTARQQLNLQFG
                     TRDLVGFGVENAPRGLCAAGCLLQYAKDTQRTTLPHIRSITMEREQDSIIMDAATRRN
                     LEITQNLAGGAENTLASVLDCTVTPMGSRMLKRWLHMPVRDTRVLLERQQTIGALQDF
                     TAELQPVLRQVGDLERILARLALRTARPRDLARMRHAFQQLPELRAQLETVDSAPVQA
                     LREKMGEFAELRDLLERAIIDTPPVLVRDGGVIASGYNEELDEWRALADGATDYLERL
                     EVRERERTGLDTLKVGFNAVHGYYIQISRGQSHLAPINYMRRQTLKNAERYIIPELKE
                     YEDKVLTSKGKALALEKQLYEELFDLLLPHLEALQQSASALAELDVLVNLAERAYTLN
                     YTCPTFIDKPGIRITEGRHPVVEQVLNEPFIANPLNLSPQRRMLIITGPNMGGKSTYM
                     RQTALIALMAYIGSYVPAQKVEIGPIDRIFTRVGAADDLASGRSTFMVEMTETANILH
                     NATEYSLVLMDEIGRGTSTYDGLSLAWACAENLANKIKALTLFATHYFELTQLPEKME
                     GVANVHLDALEHGDTIAFMHSVQDGAASKSYGLAVAALAGVPKEVIKRARQKLRELES
                     ISPNAAATQVDGTQMSLLSVPEETSPAVEALENLDPDSLTPRQALEWIYRLKSLV"
     gene            2913..3565
                     /gene="pphB"
                     /locus_tag="OO357_003904"
                     /pseudo
     CDS             2913..3565
                     /gene="pphB"
                     /locus_tag="OO357_003904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311617.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="protein-serine/threonine phosphatase"
     gene            complement(3616..4383)
                     /locus_tag="OO357_003905"
     CDS             complement(3616..4383)
                     /locus_tag="OO357_003905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417215.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5888663.1"
                     /translation="MIPVERRQIILEMVAEKGIVSIAELTDRINVSHMTIRRDLQKLE
                     QQGAVVLVSGGVQSPGRVAHEPSHQVKTALAMTQKAAIGKLAASLVLPGSCIYLDAGT
                     TTLAIAQHLIHMESLTVVTNDFVIANYLLDNSNCTIIHTGGAVCRENRSCVGEAAATM
                     LCSLMIDQAFISASSWSVRGISTPAEDKVTVKRAIASASRQRVLVCDATKYGQVATWL
                     ALPLSEFDQIITDDGLPESASRALAKQDLSLLVAKNE"
     gene            4579..5295
                     /locus_tag="OO357_003906"
                     /pseudo
     CDS             4579..5295
                     /locus_tag="OO357_003906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417216.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
BASE COUNT         1308 a         1366 c         1374 g         1247 t
ORIGIN      
        1 atcttaaagg gcactatgac tctaaagaag aagggttcgc caatcgatac aattttgcgt
       61 acttgcttca taagcatcac gcaaaagctg caaaacagca tctttcccgg aaccagcatc
      121 aagaactcgc cgttcgcttc ttcccctgaa atgattaact ccggtatcat gtgcgcctta
      181 tgtgattaca acgaaaataa aaaccatcac accccattta atatcaggga accggacata
      241 accccatgag tgcaatagaa aatttcgacg cccatacgcc catgatgcag cagtatctca
      301 agctgaaagc ccagcatccc gagatcctgc tgttttaccg gatgggtgat ttttatgaac
      361 tgttttatga cgacgcaaaa cgcgcgtcgc aactgctgga tatttcactg accaaacgcg
      421 gtgcttcggc gggagagccg atcccgatgg cggggattcc ctaccatgcg gtggaaaact
      481 atctcgccaa actggtgaat cagggagagt ccgttgccat ctgcgaacaa attggcgatc
      541 cggcgaccag caaaggtccg gttgagcgca aagttgtgcg tatcgttacg ccaggcacca
      601 tcagcgatga agccctgtta caggagcgtc aggacaacct gctggcggct atctggcagg
      661 acagcaaagg tttcggctac gcgacgctgg atatcagctc cgggcgtttt cgcctgagcg
      721 aaccggccga ccgcgaaacg atggcggcag agctgcaacg ctctaatccg gcggaactgc
      781 tgtatgcaga agatttcgcc gagatgtcgc tgattgaagg ccgtcgcggc ctgcgccgtc
      841 gcccgctgtg ggagtttgaa atcgacaccg cgcgccagca gttgaatctg caatttggca
      901 cccgcgatct ggtcggtttt ggcgtcgaga acgcgccgcg cggactttgt gctgccggtt
      961 gtctgttgca gtatgcgaaa gatacccaac gcacgaccct gccgcatatt cgttctatca
     1021 ccatggaacg tgagcaggac agcatcatta tggatgccgc gacacgtcgt aacctggaaa
     1081 ttactcagaa cctggcgggc ggtgcggaaa atacgctggc ttctgtgctc gactgcaccg
     1141 tcacgccgat gggtagtcgt atgctgaaac gctggctgca tatgccagtg cgcgataccc
     1201 gcgtgttgct tgagcgccag caaactattg gcgcattgca ggatttcacc gccgagttgc
     1261 agccggtact gcgtcaggtc ggcgacctgg aacgtattct ggcacgtctg gctttacgaa
     1321 ctgctcgccc acgcgatctg gcccgtatgc gccacgcttt ccagcaactg ccggagctgc
     1381 gtgcgcagtt agaaactgtc gatagtgcac cggtacaggc gctacgtgag aagatgggcg
     1441 agtttgccga gctgcgcgat ctgctggagc gagcaatcat cgacacaccg ccggtgctgg
     1501 tacgcgacgg tggtgttatc gcatcgggct ataacgaaga gctggatgag tggcgcgcgc
     1561 tggctgacgg cgcgaccgat tatctggagc gtctggaagt ccgcgagcgt gaacgtaccg
     1621 gcctggacac gctaaaagtt ggctttaatg cggtgcacgg ctactacatt caaatcagcc
     1681 gtgggcaaag ccatctggca cccatcaact acatgcgtcg ccagacgctg aaaaacgccg
     1741 agcgctacat cattccagag ctaaaagagt acgaagataa agttctcacc tcaaaaggca
     1801 aagcactggc actggaaaaa cagctttatg aagagctgtt cgacctgctg ttgccgcatc
     1861 tggaagcgtt gcaacagagc gcgagcgcgc tggcggaact cgacgtgctg gttaacctgg
     1921 cggaacgggc ctataccctg aactacacct gcccgacctt cattgataaa ccgggcattc
     1981 gcattaccga aggtcgccat ccggtagttg aacaagtact gaatgagcca tttatcgcta
     2041 acccgctgaa tctgtcgccg cagcgccgta tgttgattat taccggcccg aacatgggcg
     2101 gtaaaagtac ctatatgcgc cagaccgcac tgattgcgct gatggcctac atcggcagct
     2161 atgtaccggc acaaaaagtc gagattggac ctatcgatcg catctttacc cgcgtaggcg
     2221 cggcagatga cctggcttcc gggcgctcaa cctttatggt ggagatgact gaaaccgcca
     2281 atattttaca taacgccacc gaatacagtc tggtgttaat ggatgagatc gggcgtggaa
     2341 cgtccaccta cgatggtctg tcgctggcgt gggcgtgcgc agaaaatctg gcgaataaga
     2401 ttaaggcatt gacgctgttt gccacccact atttcgagct gacccagcta ccggagaaaa
     2461 tggaaggcgt cgccaacgta catctcgatg cgctggagca cggcgacact atcgccttta
     2521 tgcacagcgt gcaggacggc gcggcgagca aaagctacgg tctggcggtt gctgctctgg
     2581 cgggcgtacc gaaagaggtt attaagcgcg cacggcaaaa actgcgtgag ctggaaagca
     2641 tttcgccgaa cgccgccgcg acgcaagtgg atggtacgca gatgtctttg ctgtcagtgc
     2701 cagaagaaac ttcgcctgcg gtcgaagcac tggaaaatct cgacccggat tcactgactc
     2761 cgcgtcaggc gctggagtgg atttatcgct tgaagagtct ggtgtaataa caattcccga
     2821 tagtcttttg ctatcgggaa tattaacgac aactgacgaa tcaaataaaa ataccctgta
     2881 taataggaaa gcttatttta cagggtaaaa ccatgccatc tacacgctac caaaaaatca
     2941 atgcccatca ctatcgccat atatgggtcg ttggtgatat tcatggtgaa tatcagttat
     3001 tacaatcccg cttacatcaa ctctcttttt tccccgaaac cgacttactt atttctgtcg
     3061 gcgataatat tgatcgtggg ccggagagtc ttgacgtcct gcgcctgcta aaccagccct
     3121 ggtttatctc cgttaaaggc aaccacgaag caatggcgct ggatgcgttc gcgaccggcg
     3181 atggcaatat gtggcttgcc agcggtggtg actggttttt cgatttaaat gattgcaaca
     3241 agaggcaata gttctgttgc tgaaattcca tcaccttcca catattattg aaatcactaa
     3301 cgacataata aaatatgtca tcgcacatgc agattatccg ggtgatgaat atctctttgg
     3361 caaagaaata gcggagagcg aattactctg gcctgtttat cgtgtgcaga aatcgcttaa
     3421 tggcgagtta caacaaataa acggcgctga ttattttata tttggacata tgatgtttga
     3481 taacattcag acgttcgcta accagattta tattgatacc ggatcgccga aaagtgggcg
     3541 gctgtcattt tataaaataa ggtagtctca tgcttcttct gtgaagcatg agtaaccaga
     3601 tgttatttca ggccattatt catttttcgc taccagcaaa gagagatcct gcttcgccag
     3661 cgcgcgactg gcactctccg gcaaaccgtc gtctgtaata atctgatcaa actcgcttaa
     3721 tggtaacgcc agccatgtcg ccacctgacc atatttcgtc gcatcacaga ccaaaactcg
     3781 ctggcggctg gcactggcaa tcgcccgttt caccgtgact ttatcttccg ctggcgtaga
     3841 aatcccccgc acactccatg acgatgcaga aataaaagcc tgatcaatca tcaggctgca
     3901 cagcatggtc gcagcggctt ccccgacaca ggaacggttt tcccgacaca ctgcaccgcc
     3961 agtgtgaata attgtgcaat tactgttgtc gagcaagtag ttcgcaataa cgaaatcgtt
     4021 tgtgaccaca gtcagtgact ccatgtgaat cagatgctgt gctatcgcta acgtggtcgt
     4081 tcccgcatcc agatagatac aacttcccgg cagaacaaga cttgccgcca gcttgccaat
     4141 agccgctttt tgcgtcattg ccagcgcagt ttttacctga tgagaaggtt catgcgccac
     4201 gcgtcccgga gactggacgc ctccggacac cagcacaacg gctccctgct gctccagttt
     4261 ttgtaaatcc cgacgaatgg tcatatgtga cacattaatt ctgtccgtta gttcagcaat
     4321 actgacaatg cctttttcag ctaccatctc aaggatgatt tggcgacgct ctacgggtat
     4381 caacttttgc tccttccttt gtcctgctga cattctacgc tatttgcctg cgaaacgtgc
     4441 gcggcgcaac taacgcttag ttcacataaa ataacacaaa atgttaattt atgtgaatca
     4501 tctcaccata tcgttatctc ccagcgctta tgttcacaat atcaaacaaa atatcactta
     4561 aattaacaag gagagcagat gaaaacggga tctgagtttc atgtcggtat cgttggctta
     4621 gggtcaatgg gaatgggagc agcactgtca tgtgtccgcg caggtttttc tacctggggc
     4681 gcagacctga acagcaatgc ctgcgctacg ttgaaagagg caggtgcttg cggggtttct
     4741 gataacgccg cgacgtttgc cgaaaaactg gacgctctgc tggtgctggt ggtcaatgcg
     4801 gcctaggtta aacaggtgct gtttggtgaa acaggcgttg cacaacatct gaaacccggt
     4861 acggcagtaa tggtttcttc cactatcgct agtgctgatg cgcaagaaat tgctaccgct
     4921 ctggctggat tcgatctgga aatgctggat gcgccagttt ctggtggtgc agtaaaagcc
     4981 gctaacggtg aaatgactgt catggcctcc ggtagcgata ttgcctttga acgactggta
     5041 cccgtgctgg aagccgttgc cggaaaagtt tatcgcatag gtgcagaacc gggactaggt
     5101 tcgaccgtaa aaattattca ccagttgtta gcgggcgtac atattgctgc cggagccgaa
     5161 gcgatggcac ttgcagcccg tgcggggatc ccgctggatg tgatgtatga cgtcgtgacc
     5221 aatgccgccg gaaattcctg gatgttcgaa aaccggatgc gtcatgtggt ggatggcgat
     5281 tacaccccgc attca
//
LOCUS       DAJTRC010000179         5193 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.181, whole genome shotgun sequence.
ACCESSION   DAJTRC010000179 DAJTRC010000000
VERSION     DAJTRC010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5193
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.181"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..945
                     /gene="tauA"
                     /locus_tag="OO357_003907"
     CDS             <1..945
                     /gene="tauA"
                     /locus_tag="OO357_003907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5888664.1"
                     /translation="NTLLAALAFIAFQAQAVNVTVAYQTSAEPAKVAQADNTFAKESG
                     ASVDWRKFDSGASIVRALASGDVQIGNLGSSPLAVAASQQVPIEVFLLASKLGNSEAL
                     VVKKTISKPEDLIGKRIAVPFISTTHYSLLAALKHWGIKPGLVEIVNLQPPAIIAAWQ
                     RGDIDGAYVWAPAVNALEKDGKVLTDSEQVGQWGAPTLDVWVVRKDFAEKHPEVVKAF
                     AKSAIDAQQPYIANPDAWLKQPENISKLARLSGVPEGDVPGLVKGNTYLTPQQQTAEL
                     TGPVNKAIIDTAQFLKEQGKVPAVANDYSQYVTSRFVQ"
     gene            958..1725
                     /gene="tauB"
                     /locus_tag="OO357_003908"
     CDS             958..1725
                     /gene="tauB"
                     /locus_tag="OO357_003908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter ATP-binding subunit"
                     /protein_id="HCR5888665.1"
                     /translation="MLQISHLYADYGGKPALEDINLTLESGELLVVLGPSGCGKTTLL
                     NLIAGFVPYQHGSIQLAGKRIEGPGAERGVVFQNEGLLPWRNVQDNVAFGLQLAGIEK
                     MQRLEIAHQMLKKVGLEGAEKRYIWQLSDGQRQRVGIARALAANPQLLLLDEPFGALD
                     AFTRDQMQTLLLKLWQETGKQVLLITHDIEEAVFMATELVLLSSGPGRVLERLRLNFA
                     RRFVAGESSRSIKSDPQFIAMREYVLSRVFEQREAFS"
     gene            1722..2549
                     /gene="tauC"
                     /locus_tag="OO357_003909"
     CDS             1722..2549
                     /gene="tauC"
                     /locus_tag="OO357_003909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine ABC transporter permease TauC"
                     /protein_id="HCR5888666.1"
                     /translation="MSVLINEKLHSRRLKWRWPLSRQVTLSIGTLAVLLTVWWTVAAL
                     QLISPLFLPPPQQVLAKLLTIAGPQGFMDATLWQHLAASLTRIVLALLAAVVIGIPVG
                     IAMGLSPTVRGILDPVIELYRPVPPLAYLPLMVIWFGIGETSKILLIYLAIFAPVAMS
                     ALAGVKSAQQVRIRAAQSLGASRAQVLWFVILPGALPEILTGLRIGLGVGWSTLVAAE
                     LIAATRGLGFMVQSAGEFLATDVVLAGIAVIAIIAFLLELGLRALQRRLTPWHGEVQ"
     gene            2546..3397
                     /gene="tauD"
                     /locus_tag="OO357_003910"
     CDS             2546..3397
                     /gene="tauD"
                     /locus_tag="OO357_003910"
                     /EC_number="1.14.11.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="taurine dioxygenase"
                     /protein_id="HCR5888667.1"
                     /translation="MSERLSITPLGPYIGAQISGADLTRPLSDNQFEQLYHAVLRHQV
                     VFLRDQAITPQQQRALAQRFGELHIHPVYPHAEGVDEIIVLDTHNDNPPDNDNWHTDV
                     TFIETPPAGAILAAKELPSTGGDTLWTSGIAAYEALSVPFRQLLSWLRAEHDFRKSFP
                     EYKYRKTEEEHQRWREAVAKNPPLLHPVVRTHPVSGKQALFVNEGFTTRIVDVSEKES
                     EALLGFLFAHITKPEFQVRWRWQPNDIAIWDNRVTQHYANADYLPQRRIMHRATILGD
                     KPFYRAG"
     gene            complement(3503..4477)
                     /gene="hemB"
                     /locus_tag="OO357_003911"
     CDS             complement(3503..4477)
                     /gene="hemB"
                     /locus_tag="OO357_003911"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308450.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HCR5888668.1"
                     /translation="MTDLIQRPRRLRKSPALRAMFEETTLSLNDLVLPIFVEEEIDDY
                     KAVEAMPGVMRIPEKHLAREIERIANAGIRSVMTFGISHHTDETGSDAWREDGLVARM
                     SRICKQTVPEMIVMSDTCFCEYTSHGHCGVLCEHGVDNDATLENLGKQAVVAAAAGAD
                     FIAPSAAMDGQVQAIRQALDAAGFKDTAIMSYSTKFASSFYGPFREAAGSALKGDRKS
                     YQMNPMNRREAIRESLLDEAQGADCLMVKPAGAYLDIVRELRERTELPIGAYQVSGEY
                     AMIKFAALAGAIDEEKVVLESLGSIKRAGADLIFSYFALDLAEKKILR"
     gene            5001..>5193
                     /locus_tag="OO357_003912"
     CDS             5001..>5193
                     /locus_tag="OO357_003912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888669.1"
                     /translation="MHSWKKKLVVSQLALACTLAITSQANAATNDISGQTYNTFHHYN
                     DATYADDVYYDGYVGWNNYA"
BASE COUNT         1195 a         1305 c         1455 g         1238 t
ORIGIN      
        1 aacacacttc ttgccgcact ggcattcatc gcttttcagg cacaggcggt gaacgtcacc
       61 gtggcgtatc aaacctcagc cgaaccggcg aaagtggctc aggccgacaa cacctttgct
      121 aaagagagcg gggcgagcgt ggactggcgt aagtttgaca gcggagccag catcgtgcgg
      181 gcgctggctt ctggcgacgt gcaaatcggc aacctcggtt ccagcccatt agcggttgca
      241 gccagccaac aggtgccgat tgaagtcttc ttgctggcgt caaaactggg taactccgaa
      301 gcgctggtgg taaagaaaac tatcagcaaa cccgaagatt tgattggtaa acgcattgcc
      361 gtaccgttta tctccaccac ccactacagc ctgctggcgg cgctgaaaca ctgggggatt
      421 aaaccagggc tagtggagat tgtgaacctg cagccgcccg cgattatcgc cgcatggcag
      481 cggggtgata ttgatggtgc ttatgtctgg gcaccggcgg ttaacgccct ggaaaaagac
      541 ggcaaggtgc tgaccgattc tgaacaggtc gggcagtggg gtgcgccaac gctggacgtc
      601 tgggtggtgc gcaaagattt tgccgagaaa catcctgagg tcgtgaaagc gttcgccaaa
      661 agcgccatcg atgcacagca accgtacatt gctaaccctg acgcgtggct gaaacagccg
      721 gaaaacatca gcaaactggc gcgtttaagc ggcgtgcctg aaggtgacgt tccggggctg
      781 gtgaagggaa atacctatct gacgccgcag caacaaacgg cagaactgac cggaccggtg
      841 aacaaagcaa tcatcgacac cgcgcagttt ttgaaagagc agggcaaggt cccggctgta
      901 gcgaatgatt acagccagta cgttacctcg cgcttcgtgc aataaaagga ggcgcagatg
      961 ctgcaaatct ctcatctcta tgccgattat ggcggcaaac cggcactgga agatatcaac
     1021 ctgacgctgg aaagcggcga gctactggtg gtgctggggc cgtccggctg cggtaaaacc
     1081 accctgctga atctgattgc cggttttgtg ccttatcagc atggcagcat tcaactggcg
     1141 ggtaagcgta ttgagggacc gggagcagag cgtggcgtag tttttcagaa tgaagggctg
     1201 ctaccgtggc gcaatgtaca ggacaacgtg gcgttcggcc tgcaattggc aggtatagag
     1261 aaaatgcagc gactggaaat cgcgcaccag atgctgaaaa aagtggggct ggaaggcgca
     1321 gaaaaacgct atatctggca gctttccgat ggtcaacgtc agcgggtggg gattgcccgc
     1381 gcgctggcgg cgaatcccca gctgttatta ctcgacgaac cgtttggtgc gctggacgcc
     1441 ttcacccgcg accagatgca aactctgctg ctgaaactct ggcaggagac gggcaagcag
     1501 gtgctgttga ttacccacga tatagaagaa gcggtgttta tggcgactga actggttctg
     1561 ctttcatccg gccctggccg tgtgctggag cggctgcggc tcaactttgc tcgccgcttt
     1621 gttgcgggag agtcgagccg cagcatcaag tccgatccac aattcatcgc catgcgcgaa
     1681 tatgttttaa gccgcgtatt tgagcaacgg gaggcgttct catgagtgtg ctcattaatg
     1741 aaaaactgca ttcgcggcgg ctgaaatggc gctggccgct ctcgcgtcag gtgaccttaa
     1801 gcattggcac gttagcggtt ttactcactg tatggtggac ggtggcggcg ctgcaactga
     1861 ttagcccgct gtttttgccg ccgccgcaac aagtactggc aaaactgctc accattgccg
     1921 gaccgcaggg atttatggac gccacgctgt ggcagcatct ggcagccagc ctgacgcgca
     1981 ttgtgctggc gctactcgcg gcggtggtga tcggtattcc ggtcgggatc gccatggggc
     2041 taagcccgac ggtgcgcggc attctggatc cggtaatcga actttatcgt ccggtgccac
     2101 cgctggctta tttgccgctg atggtgatct ggtttggtat tggtgaaacc tcgaagatct
     2161 tactgatcta tttagcgatt tttgcgccgg tggcgatgtc ggcgctggca ggggtgaaaa
     2221 gcgcgcagca ggttcgcatt cgtgccgccc agtcgctggg tgccagccgt gcgcaggtgc
     2281 tgtggtttgt cattttgccc ggcgcgctgc cggaaatcct caccggatta cgcattggtc
     2341 tgggtgtggg ctggtctacg ctggtagcgg cggagctgat tgccgcgaca cgcggtttag
     2401 gatttatggt tcagtcagcg ggtgaatttc tcgcgactga cgtggtactg gcggggatcg
     2461 cggtgattgc gattatcgcc tttcttttag aactgggtct gcgcgcgtta cagcgccgtc
     2521 tgacgccctg gcatggagaa gtacaatgag tgaacgtctg agcattaccc cgctggggcc
     2581 gtatatcggc gcacaaattt cgggtgccga cctgacgcgc ccgttaagcg ataatcagtt
     2641 tgaacagctt taccatgcgg tgctgcgcca tcaggtggta tttctgcgcg atcaagctat
     2701 tacgccgcag cagcaacgcg cgctggccca gcgttttggc gaactgcata ttcaccctgt
     2761 ttaccctcat gccgaagggg ttgacgagat catcgtgctg gatacccata acgataatcc
     2821 gccggataac gacaactggc ataccgatgt gacatttatt gaaacgccac ccgcaggggc
     2881 gattctggca gctaaagagt taccttcgac cggcggtgat acgctctgga ccagcggtat
     2941 tgcggcctat gaggcgctct ctgttccctt ccgccagctg ctgagctggc tgcgtgcgga
     3001 gcatgatttc cgtaaatcat tcccggaata caaataccgc aaaaccgagg aggaacatca
     3061 acgctggcgc gaggcggtcg cgaaaaatcc tccgttgcta catccggtgg tgcgaacgca
     3121 tccggtgagc ggtaaacagg cgctgtttgt gaatgaaggc tttactacgc gaattgtcga
     3181 tgtcagcgag aaagagagcg aagccttgtt aggcttttta ttcgcccata tcactaaacc
     3241 ggagtttcag gtgcgttggc gctggcaacc gaatgatatt gcgatttggg ataaccgcgt
     3301 gactcagcac tatgccaatg ccgattacct gccacagcgg cggattatgc atcgggcgac
     3361 gatccttggg gataaaccgt tttatcgggc ggggtgatac gagtgcggac ggttccctcg
     3421 cccccttggg gagagggtta gggtgagggg gcgttcactg tactttcaac aggttaactc
     3481 cccctttctg agaggaaaaa aattaacgca gaatcttctt ctcagccaaa tccagcgcaa
     3541 agtagctgaa aatcagatcc gcacccgcac gcttaattga acctaagctt tcgagcacga
     3601 ctttctcttc atctatagca cccgccagcg cggcgaactt aatcatcgcg tactcaccgc
     3661 tcacctgata cgcgccaatc ggcaattcag tacgttcacg cagctcacgc acgatgtcga
     3721 ggtacgctcc agcaggttta accatcaggc agtctgcgcc ctgggcttca tccagcagtg
     3781 actcacgaat cgcctcgcga cggttcattg ggttcatctg atagcttttg cggtcgcctt
     3841 ttaatgcgct tcctgcagct tcacggaacg ggccataaaa ggaagaggcg aacttggtcg
     3901 aatacgacat aatcgccgta tctttaaagc ctgcagcgtc cagcgcctga cgaatcgcct
     3961 gtacctggcc gtccatcgcg gcggaagggg cgatgaagtc tgcgcctgca gcagctgcaa
     4021 ccacggcttg cttgcctaaa ttttccagag tcgcgtcgtt gtcgacgcca tgctcgcaca
     4081 gcacaccgca gtgaccgtga gaagtgtatt cacagaaaca agtgtctgac atgacgatca
     4141 tttctggcac agtctgcttg cagatgcgcg acatacgcgc caccagtcca tcttcccgcc
     4201 aggcgtcgct gccggtttca tcggtatggt gagagatgcc aaaagtcatt acggaacgga
     4261 taccggcgtt ggcgatgcgt tcaatttcgc gtgccagatg tttctctgga atgcgcatca
     4321 cgcctggcat ggcttcaacg gctttgtagt cgtcaatttc ttcttcaaca aagatcggca
     4381 acaccaggtc gttaaggcta agtgttgtct cttcaaacat agcgcgcagc gcaggagatt
     4441 tgcgcaggcg acgagggcgt tggattaagt ctgtcatggt ctgcctgatg tttgtggaat
     4501 taaggtgcac agtatacctg aagcggggca ggagtgtttt acgaaagttg tcgcgatgtt
     4561 gacaagaaga gaatgaaaga gagaccagga catctttgta atagaaatag caataatttc
     4621 atattcactg aaaatatttt taatctttat ttatagctct tggttattat tttttggcgt
     4681 ttggttgtgc tgctataaat tgaataattc aaactttgtt gtatattgat aagatattta
     4741 tctggtttgt atttttgata tttataacgc gtatgcatca aaaataacat tatctgcaac
     4801 atactgaaat atcgtccttt tatcaatttg atgcgtgttt ttctccataa attatatatg
     4861 tccacattcg gacttagggg aaagaataat tgaaccattc gtctgtaacg cagcataatc
     4921 gttagcgcga aacataatat gttttctatg cattgataat tgatggatca acttattacg
     4981 tccctgagga gggatgacaa atgcactcct ggaaaaagaa acttgtagta tcacaattag
     5041 cattggcttg cactctggct atcacctctc aggctaatgc agcgaccaac gatatttctg
     5101 gtcaaactta caatactttc catcactaca acgacgccac ctatgctgat gacgtttact
     5161 atgatggtta tgtaggctgg aacaactatg ccg
//
LOCUS       DAJTRC010000180         5186 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.182, whole genome shotgun sequence.
ACCESSION   DAJTRC010000180 DAJTRC010000000
VERSION     DAJTRC010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5186)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5186)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5186
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.182"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(32..982)
                     /gene="cbl"
                     /locus_tag="OO357_003913"
     CDS             complement(32..982)
                     /gene="cbl"
                     /locus_tag="OO357_003913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator Cbl"
                     /protein_id="HCR5888670.1"
                     /translation="MNFQQLKIIREAARQDYNLTEVANMLFTSQSGVSRHIRELEDEF
                     GIEIFVRRGKRLLGMTEPGKALLVIAERILNEASNVRRLADLFTNDTSGVLTIATTHT
                     QARYSLPEVIKAFRELFPEVRLELIQGSPQEIATLLQNGEADIGIASERLSNDPQLVA
                     FPWFCWHHSLLVPLDHPLTQITPLTLESIAKWPLITYRQGITGRSRTDDAFTRKGLPA
                     DIVLSAQDSDVIKTYVALGLGIGLVAEQSSGEQEEENLIRLDTRHLFDANTVWLGLKR
                     GQLQRNYVWRFLELCNAGLSVEDIKRQVMENSEEEIDYQI"
     gene            complement(1084..2001)
                     /gene="nac"
                     /locus_tag="OO357_003914"
     CDS             complement(1084..2001)
                     /gene="nac"
                     /locus_tag="OO357_003914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707891.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen assimilation transcriptional regulator
                     NAC"
                     /protein_id="HCR5888671.1"
                     /translation="MNFRRLKYFVKIVDIGSLTQAAEVLHIAQPALSQQVAILEGELN
                     QQLLIRTKRGVTPTDAGKILYTHARAILRQCEQAQLAVHNVGQSLSGQVSIGFAPGTA
                     ASSITMPLLQAVRAEFPEVVIYLHENSGAVLNEKLINHQLDMAVIYEHSPVAGVSSQA
                     LLKEDLFLVGTQDCPGQNVDVNAIAQMNLFLPSDYSAVRLRVDEAFSLRRLTAKVIGE
                     IESIATLTAAIASGMGVAVLPESAARSLCGAVNGWMSRITTPSMSLSLSLNLPARANL
                     SPQAQAVKELLMSVISSPVMEKRQWQLVS"
     gene            2329..2404
                     /locus_tag="OO357_003915"
     tRNA            2329..2404
                     /locus_tag="OO357_003915"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2362..2364,aa:Asn,seq:gtt)
     gene            complement(2460..3395)
                     /gene="ldtA"
                     /locus_tag="OO357_003916"
     CDS             complement(2460..3395)
                     /gene="ldtA"
                     /locus_tag="OO357_003916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HCR5888672.1"
                     /translation="MMRRVNILCSFALLFASQNSLAVTYPLPPEGSRLVGQSLTVTVP
                     DHNTQPLETFAAQYGQGLSNMLEANPGADVFLPKPGSQLTIPQQLILPATVRKGIVVN
                     VAEMRLYYYPPDSNTVEVFPIGIGQAGRETPRNWVTTVERKQEAPTWTPTPNIRREYA
                     KRGESLPAFVPAGPDNPMGLYAIYIGRLYAIHGTNANFGIGLRVSQGCIRLRNDDIKY
                     LFDNVPVGTRVQIIDQPVKYTTEPDGSKWLEVHEPLSRNRAEYESDRKVPLPVTPSLR
                     AFINGQEVDVNRANAALQHRSGMPVQISSGSRQMF"
     gene            complement(3457..4536)
                     /gene="cobT"
                     /locus_tag="OO357_003917"
     CDS             complement(3457..4536)
                     /gene="cobT"
                     /locus_tag="OO357_003917"
                     /EC_number="2.4.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide--dimethylbenzimidazole
                     phosphoribosyltransferase"
                     /protein_id="HCR5888673.1"
                     /translation="MQIIADLLNTIPAINSAAMSRAQRHVDGLFKPVGSLGKLEALAI
                     QLAGMPGVNGIPHVGKKAVLVMCADHGVWEEGVAISPKEVTAIQAENMTRGTTGVCVL
                     AAQAGANVHVIDVGIDTAEPIPGLINMRVARGSGNIASAPAMSRRQAEKLLLDVICYT
                     RELAKNGVTLFGVGELGMANTTPAAAIVSTITGRDPEEVVGIGANLPTDKLANKIDVV
                     RRAITLNQPNPQDGVDVLAKVGGFDLVGIAGVMLGAASCGLPVLLDGFLSYAAALAAC
                     QMSPAIKPYLIPSHLSAEKGARIALSHLGLEPYLNMDMRLGEGSGAALAMSIIEAACA
                     IYNNMGELAASNIVLPGNTTSDLNS"
     gene            complement(4548..>5186)
                     /gene="cobS"
                     /locus_tag="OO357_003918"
     CDS             complement(4548..>5186)
                     /gene="cobS"
                     /locus_tag="OO357_003918"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707894.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HCR5888674.1"
                     /translation="TFPLIGLLLGAISGLVFMVLQAWCGAPLAALFSVLVLALMTGEF
                     HLDGLADTCDGVFSARSRDRMLEIMRDSRLGTHGGLALIFVVLAKILVLSELALRGEP
                     ILASLAAACAVSRGTAALLMYRHRYAREEGLGNVFIGKIDGRQTCVTLGLAAIFAAVL
                     LLGMHGVAAMVVTMVAIFILGQLLKRTLGGQTGDTLGAAIELGELVFLLALL"
BASE COUNT         1375 a         1399 c         1144 g         1268 t
ORIGIN      
        1 tgaaaagccc gcttttatag cgggcttttt gctatatctg ataatcaatt tcctcttcac
       61 tgttttccat cacctggcgc ttgatatcct caactgacag tcctgcatta caaagttcca
      121 gaaagcgcca aacatagtta cgctgaagtt gtcctcgctt cagtcccaac cagacagtat
      181 tagcatcgaa aagatgccgc gtatccaggc ggattaaatt ctcttcctct tgttcgccgc
      241 tggattgttc ggcaactaat ccgatcccaa gcccaagagc aacataggtt ttaatgacat
      301 cagaatcctg tgcacttaat acaatatctg ccggcaaacc tttgcgggta aatgcgtcat
      361 cagtgcgtga gcgccccgta attccctgtc ggtaagtaat taacggccac ttcgctattg
      421 attccagcgt caatggtgta atttgcgtta agggatgatc gagtggaaca agcaaactat
      481 ggtgccaaca aaaccatggg aaggcgacga gctgcgggtc attactcaaa cgctcgctgg
      541 cgataccaat atcagcttcg ccattttgca acaacgtcgc aatttcctgt ggcgaaccct
      601 ggattagctc gagccgaacc tccgggaaaa gttcgcgaaa agctttaatg acctctggca
      661 agctataacg tgcctgagta tgcgtcgttg caatagtgag aacaccagac gtatcgttgg
      721 taaacaggtc tgcaagccga cgaacattac tggcttcatt cagaatacgt tctgcaatga
      781 ccagtaatgc tttgcccggt tcagtcatcc ccagcagtcg cttacctcgt cgaacaaata
      841 tttcgatgcc aaattcatcc tccagttccc gaatatgacg gctaacgcct gactgtgagg
      901 taaaaagcat attcgcaacc tctgtcaggt tgtaatcctg acgtgcagcc tcgcggatta
      961 tctttagttg ttggaaattc acggtaaact ccgggcagtt cagatatccc gttattgtta
     1021 aagtctaatg cccggcataa caaataataa aaacccgcat cttattccat cccgatataa
     1081 cgcttagctc accaattgcc actgcctttt ttccatcact ggagaactaa tcactgacat
     1141 taacaactct ttcactgcct gtgcctgtgg tgacaagttt gctctggcgg gtaaatttaa
     1201 ggacaaagag agactcattg aaggagtggt aatgcgtgac atccacccat ttactgcgcc
     1261 acataacgaa cgcgcagccg attcaggtaa tactgcaacg cccatgccgc tggcaatcgc
     1321 tgcggtaagc gtggcaatag actcaatttc accaatgact tttgccgtga gccgccgtag
     1381 ggaaaaagcc tcatcaacac gaagtctaac agcgctgtaa tcactgggga gaaaaaggtt
     1441 catttgcgca atagcattaa catcaacgtt ttgccccggg caatcttgag ttcctaccag
     1501 gaaaagatct tctttcagca aagcctgact ggatacccca gccacagggg aatgctcata
     1561 aatcaccgcc atatcgagtt ggtgatttat cagtttttcg ttaagcactg caccactatt
     1621 ttcatgaaga tagataacga cctccgggaa ttcagcgcga accgcctgta ataagggcat
     1681 ggtgatggat gacgcagcgg ttcctggcgc aaagccaatc gagacttgcc ccgataatga
     1741 ctgaccaacg ttatgcaccg ccagttgggc ctgctcacac tgacgtaaaa tggcccgtgc
     1801 atgggtatag agaatttttc cggcgtctgt tggcgtaacg ccccgttttg tgcgaatcaa
     1861 aagctgttga tttaactcac cttccagtat ggcaacctgt tggctgagcg ctggttgtgc
     1921 gatatgcaat acttcagcag cctgggtcag gctaccaata tctacaattt ttacgaagta
     1981 tttcaggcgt ctgaagttca tgttgcctcc ggtttttaag aatcggccca agtgccgcca
     2041 ttacttacaa ccagtatgca agatgcctgc cagttctatt ttggcgttga tgcgcaagct
     2101 aaccaactgt caaataagag attatgatag attcgtcatt ggcccttttt atcagcagtc
     2161 gcattcacct gccctataaa aggagggtat gcaccacgat ggttcattac ccaataagat
     2221 tgaaagctca ccactttgtt gaaattggca gcaaacaaac aaaaaaaatg catttcaccc
     2281 tttgacatca ccatgcactg ccattaatat gcgccccgtt cacacgattc ctctgtagtt
     2341 cagtcggtag aacggcggac tgttaatccg tatgtcactg gttcgagtcc agtcagagga
     2401 gccaaattca aaaaagcctg ctttcgagca ggctttttac tttctaatta ccaacgctct
     2461 taaaacatct gtcttgaacc agaactaatt tgcacaggca ttcccgatcg atgttgcaac
     2521 gcagcatttg cgcgatttac atcaacttct tgcccgttga taaacgcccg caaagatggg
     2581 gttaccggca atggcacttt tcggtcagac tcatattctg cacgattgcg cgacagcggc
     2641 tcatgaactt ccagccactt tgaaccatcc ggttccgttg tgtatttcac tggctgatca
     2701 ataatttgta cacgagtgcc tacaggaaca ttatcaaaca gatatttgat atcgtcatta
     2761 cgcagacgaa tacagccctg acttacacgg agcccaatac caaaattggc attggtaccg
     2821 tggatggcat acaacctgcc aatataaatc gcgtacagcc ccatggggtt atcgggcccc
     2881 gcaggaacaa atgcgggcaa actctcccct cgtttcgcat attcgcgccg gatgttcggc
     2941 gttggcgtcc aggttggtgc ttcttgttta cgttcaacgg tagtcaccca gttacgcggg
     3001 gtttctcgcc cagcctggcc gataccaata ggaaagactt ccactgtatt actgtctggc
     3061 ggatagtaat aaagacgcat ttcagcgacg ttaaccacaa tccctttacg aacagtggcg
     3121 ggcaaaatca gttgctgcgg aatggtgagt tgtgagccag gcttcggcaa aaaaacatca
     3181 gcgcccggat tcgcttccag catattactt aacccttgcc cgtattgtgc ggcaaaagtc
     3241 tccagcggct gagtattgtg atcaggaaca gttacagtaa gcgactgccc cactaaacgg
     3301 ctaccctctg gaggtaatgg ataagttacc gccaggctat tttggctggc aaaaagcaga
     3361 gcaaatgagc aaagaatatt tacacgacgc atcatgtccc tttcctaagt cgcgaatact
     3421 atccgataag tatagctttt atcagacttt tcgtttttag ctgttcaaat cagaagtcgt
     3481 attccccggt agaacaatat tactggcagc aagttcgccc atgttgttgt atatcgcaca
     3541 agcagcttcg atgatggaca tcgccagagc tgcaccactc ccctcaccta aacgcatatc
     3601 catattgaga taaggctcca gccccaaatg cgagagcgct atacgcgcgc ctttttctgc
     3661 cgacaagtga gaaggaatga gatacggttt gattgcagga gacatctggc aggctgcgag
     3721 cgcagcagca taagaaagaa atccatccag caacacgggt aaaccgcagg aagcagcacc
     3781 taacatcact ccagctattc cgaccaaatc aaatccaccc acttttgcca ggacatcaac
     3841 accatcctga ggatttggtt gattcaacgt aatcgcccga cgcacaacat caattttatt
     3901 agccagttta tctgtcggca ggtttgcgcc aatcccaacc acttcttcag gatcccggcc
     3961 agtgattgtg ctgactattg ccgctgccgg tgtcgtgttt gccatcccca gttcacctac
     4021 accaaacagc gtgacaccgt ttttcgccag ctcccgcgta taacatatga cgtccaaaag
     4081 caacttttca gcctgacggc gactcattgc cggagctgaa gcaatattgc cgctacctcg
     4141 tgcgacacgc atgttgataa gcccggggat aggctcagca gtatcaatac caacatcaat
     4201 tacgtggacg ttagcgcccg cttgtgctgc cagcacacac acgccggttg ttccacgggt
     4261 catattttca gcctgtatgg ctgtcacttc ttttggagaa atagcgaccc cttcctccca
     4321 gacgccgtga tcggcacaca taaccagtac cgcttttttg cccacatgcg gtatgccatt
     4381 cacccccggc attcctgcca gttgtatggc aagcgcctcc agctttccta ggctaccaac
     4441 aggtttgaat aacccgtcaa catgccgttg tgcacgcgac atagcggcag aattgatggc
     4501 tgggatcgta ttcagtaaat cggcaattat ttgcatgtca cgtttcttta tagcagtgcc
     4561 agcaggaaga ccagttcacc aagctctatg gctgctccca gcgtatcgcc cgtttgcccg
     4621 cccagcgtac gtttgagaag ctgaccgagg atgaaaatcg ccaccatcgt caccaccata
     4681 gcagccacac catgcatacc cagcaataat acggcagcaa aaatagcggc taagccgagt
     4741 gtgacgcagg tttgtcgccc atcaattttg ccaatgaaca cattgccaag cccttcttcc
     4801 cgcgcataac gatgacgata catcaataat gcggcagtcc cgcggctgac cgcacatgct
     4861 gccgccagcg atgcaaggat cggttcgcca cgcagagcca actcgcttaa caccagaatc
     4921 tttgccagta ccacaaaaat caatgccaga ccgccgtggg tgcctaaacg gctatcacgc
     4981 atgatttcca acatgcgatc gcgactacgt gcagaaaata cgccatcaca ggtatcagcg
     5041 aggccgtcca gatggaattc cccggtcatc agcgccagca caagtacgct aaacagtgcc
     5101 gccagtggtg cgccacacca tgcctgcagc accatgaaga ccagcccgct aatcgcgcca
     5161 agcaataatc caatcaaagg aaaagt
//
LOCUS       DAJTRC010000181         5172 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.183, whole genome shotgun sequence.
ACCESSION   DAJTRC010000181 DAJTRC010000000
VERSION     DAJTRC010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.183"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..736
                     /locus_tag="OO357_003919"
     CDS             <1..736
                     /locus_tag="OO357_003919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5888675.1"
                     /translation="SQHISCWNDYGGWYDTDHINLVQGSAFAGSLQSYKGSLYWNNVT
                     YPFPLTTNTNVLDIGDKTPMPLPLKLYITPVGAAGGVVIKAGEVIARIHMYKIATLGS
                     GNPRNFTWNIISNNSVVMPTGGCTVDSRNVTVDLPDFPGSAEIPLGVYCSSEQKLSFY
                     LSGATTDSSRQVFANTAPDATKASGVGVTLMRNGKILATGENVSLGTVNKSKVPLGLS
                     ATYGQTGNKVSAGTVQSVIGVTFIYE"
     gene            1070..3349
                     /gene="ydeP"
                     /locus_tag="OO357_003920"
     CDS             1070..3349
                     /gene="ydeP"
                     /locus_tag="OO357_003920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid resistance putative oxidoreductase YdeP"
                     /protein_id="HCR5888676.1"
                     /translation="MKKKIESYQGAAGGWGAVKSVANAVRKQMDIRHDVIAMFDMNKP
                     EGFDCPGCAWPDPKNSASFDICENGAKAIAWEVTDKQVNASFFAENTVQSLLTWGDHE
                     LEAAGRLTQPLKYDAVSDCYKPLSWQQAFDEIGARLQSYSDPNQVEFYTSGRTSNEAA
                     FLYQLFAREYGSNNFPDCSNMCHEPTSVGLAASIGVGKGTVLLEDFEKCDLVICIGHN
                     PGTNHPRMLTSLRALVKRGAKMIAINPLQERGLERFTAPQNPFEMLTNSETQLASAYY
                     NVRIGGDMALLKGMMRLLIERDDAASAAGRPSLLDDEFIQTHTVGFDELRRDVLNSEW
                     KDIERISGLSQTQIAELADAYAAAERTIICYGMGITQHEHGTQNVQQLVNLLLMKGNI
                     GKPGAGICPLRGHSNVQGDRTVGITEKPSAEFLARLGERYGFPPPHAPGHAAIASMQA
                     ICTGQARALICMGGNFALAMPDREASAVPLTQLDLAVHVATKLNRSHLLTARHSYILP
                     VLGRSEIDMQKSGAQAVTVEDSMSMIHASRGVLKPAGVMLKSECAVVAGIAQAALPQS
                     VVAWEYLVEDYDRIRNDIEAVLPEFADYNQRIRHPGGFHLINAAAERRWMTPSGKANF
                     ITSKGLLEDPSSAFNSKLVMATVRSHDQYNTTIYGMDDRYRGVFGQRDVVFMSAKQAK
                     ICRVKNGERVNLIALTPDGKRSSRRMDRLKVVIYPMADRSLVTYFPESNHMLTLDNHD
                     PLSGIPGYKSIPVELEPSN"
     gene            3596..3799
                     /gene="safA"
                     /locus_tag="OO357_003921"
     CDS             3596..3799
                     /gene="safA"
                     /locus_tag="OO357_003921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system connector SafA"
                     /protein_id="HCR5888677.1"
                     /translation="MHATTVKNKITQRDNYKEIMSVIVVVLLLTLTLIAIFSAIDQLS
                     ISEMGRIARDLTHFIINSLQDWK"
     gene            3867..4628
                     /gene="ydeO"
                     /locus_tag="OO357_003922"
     CDS             3867..4628
                     /gene="ydeO"
                     /locus_tag="OO357_003922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acid stress response transcriptional regulator
                     YdeO"
                     /protein_id="HCR5888678.1"
                     /translation="MSLVCSVIFIHHAFNANILDKDYAFSDGEILMVDNAVRTHFEPY
                     ERHFKEIGFNENTIKKYLQCTNIQTVTVPVPAKFLRASNVPTGLLNEMIAYLNSEERN
                     HHNFSELLLFSCLSIFAACKGFITLLTNGVLSVSGKVRNIVNMKLAHPWKLKDICDCL
                     YISESLLKKKLKQEQTTFSQILLDARMQHAKNLIRVEGSVNKIAEQCGYASTSYFIYA
                     FRKHFGNSPKRVSKEYRCQRHTGMNTGNTMSALAI"
     gene            5030..>5172
                     /locus_tag="OO357_003923"
     CDS             5030..>5172
                     /locus_tag="OO357_003923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase"
                     /protein_id="HCR5888679.1"
                     /translation="MKSALKKSVVSTSISLILASGMAAFAAHAADDVKLKATKTNVAF
                     SDFT"
BASE COUNT         1478 a         1102 c         1154 g         1438 t
ORIGIN      
        1 gtctcagcat ataagttgct ggaatgatta cggcggctgg tacgacactg atcatataaa
       61 cctggtacaa ggctcagctt tcgcaggctc cctacagtca tataaaggat cgctgtactg
      121 gaataatgtg acctacccct tcccgttgac gacaaatacc aacgtattgg atattggtga
      181 taagacaccg atgccattac cgttaaaact ctatattacc ccagttgggg cggccggtgg
      241 agttgtcata aaagcggggg aagtcattgc ccgtattcat atgtataaaa ttgcaacatt
      301 aggaagcgga aatccgcgta attttacgtg gaatattatt tcaaataaca gtgttgttat
      361 gccaacaggc ggctgcaccg ttgatagtcg taatgtcacc gtggatctcc cggactttcc
      421 tggaagcgca gaaatacccc ttggcgtata ttgctcaagc gaacaaaagc tctcatttta
      481 tctttcgggt gccaccactg acagtagccg acaagtgttt gctaacacag cacctgatgc
      541 aacaaaagca tcgggagtgg gtgtcactct aatgcgtaat ggaaaaatac tggctacggg
      601 agaaaatgtc tccctgggga cagtaaataa aagcaaagta cctctgggtt taagcgcaac
      661 ctatggacaa acaggaaaca aggtttcagc cggaaccgtc cagtcagtca ttggtgtcac
      721 ttttatctat gaataattgt gattttgacc ttagtctatg cgctccattc acgaaaattg
      781 cgcggataat tattttgtga aggctattat cctacacctg taagaaaatc cgcgcacaat
      841 tgaagccaga gtcttatttt ttatattgga aaatatgtta atttaaacac tatgagagat
      901 gatgcttaac ttatcgtatt taatctattg tttaacgata atagattaaa aatagaaaga
      961 gagtagtccc ttactcttat atatccatgt tggcgataat ccctttgtat gtactgtgca
     1021 tcatcgctat tacaaatcct aataattcat ttccacacag gataagtaga tgaagaaaaa
     1081 aattgaatcc taccagggtg ctgcaggtgg ttggggtgct gttaaatccg tagcgaatgc
     1141 agtacgtaag cagatggata tacgccatga tgttattgcc atgtttgaca tgaataagcc
     1201 agagggcttt gactgtccgg gttgtgcatg gccagatcct aagaacagtg cgtcattcga
     1261 catttgtgaa aacggcgcaa aagcaatcgc ttgggaagtc acggataagc aggtaaatgc
     1321 ctctttcttt gctgagaata cggttcaatc attacttacc tggggagatc acgagcttga
     1381 ggctgcgggg cgtctcactc agcctttgaa atatgatgcc gtcagcgact gttacaagcc
     1441 attaagctgg caacaagctt tcgacgaaat tggcgcacgc cttcaaagct atagtgatcc
     1501 caatcaggtt gaattctata cttcgggccg cacttccaat gaagctgcct ttctttatca
     1561 gctttttgcc cgtgaatacg ggagcaataa ctttcccgac tgctccaaca tgtgccatga
     1621 accgacaagc gtgggtttgg cagcgagtat cggtgtaggt aaagggaccg tgttgctgga
     1681 agactttgag aagtgcgatt tagtcatttg cattgggcat aaccctggta caaaccaccc
     1741 tcgcatgctg acttcgttgc gcgctttagt gaaacgggga gcgaaaatga tcgccatcaa
     1801 tcctctacag gaacgtggcc tggagcgatt taccgcaccg caaaacccgt ttgaaatgct
     1861 gacgaactct gagactcagt tggccagtgc ctactacaac gtgcgcattg gtggcgatat
     1921 ggcgttgctc aaggggatga tgcgcctgtt aattgagcgc gatgatgctg caagtgccgc
     1981 aggtcggccc tcattgcttg atgacgaatt tattcaaacg cataccgtcg gctttgacga
     2041 gctacgccgt gacgttctca attccgagtg gaaagatatc gaacgtattt ctggactaag
     2101 tcagacacaa atcgccgaac tggctgacgc atatgccgct gccgaacgca ccattatctg
     2161 ttacggaatg gggatcactc agcacgaaca tggtacccag aacgtgcagc aactggtcaa
     2221 tttgctgttg atgaaaggta acattggcaa gcctggtgcg ggtatctgcc cactacgtgg
     2281 tcactctaat gtacagggcg accgaaccgt cggtatcacc gagaaaccgt ctgcagagtt
     2341 tctggctcgc ctgggtgagc gctatggctt ccccccacct catgcacctg gacatgctgc
     2401 aattgccagc atgcaagcaa tatgtacggg gcaggctcga gcattgatct gcatgggggg
     2461 caactttgca ctggcaatgc cagatcggga agcgagcgct gtaccgttaa cgcaattaga
     2521 tttggcggta cacgtagcca ctaagcttaa ccgctctcat ctgctgaccg cacggcatag
     2581 ctatattctg ccggttctgg ggcgtagcga gattgatatg caaaaaagcg gtgcgcaggc
     2641 ggtaaccgtt gaggattcaa tgtcgatgat tcatgcctcg cgtggcgtgt taaaacccgc
     2701 cggtgtaatg ctgaaatcag agtgtgcagt ggtcgcggga atcgcgcagg cagcactacc
     2761 ccagagcgtg gtagcctggg agtatctggt ggaagattat gatcgcattc gcaatgacat
     2821 tgaagctgtg ctgccagagt tcgccgacta taaccagcgc atccgtcatc ccggtggttt
     2881 tcacctgata aatgcagctg ctgaaaggcg ctggatgacg ccgtcaggta aggctaattt
     2941 cattaccagc aaagggctgt tagaagatcc ctcttcagcg tttaacagta agctggtcat
     3001 ggcgacagta cgcagccacg atcagtacaa cacgacgatt tatggtatgg atgatcgcta
     3061 tcgaggggta ttcggtcaac gagatgtggt ctttatgagt gctaaacaag ctaaaatttg
     3121 ccgtgtaaaa aacggcgaaa gagttaatct tattgcgctt acgccagacg gtaagcgcag
     3181 ctcacgccgc atggatagat taaaagtggt catttaccct atggctgacc gctcactggt
     3241 gacctatttt ccagaatcga atcacatgct aacacttgat aaccacgatc cattaagtgg
     3301 tattcctggc tataaaagta ttccggttga attagaacca tcaaattaat gcctcttctc
     3361 atttcttctg ctgtcatccg cacagcagaa gaattcctca ttgactatta ttgcgcaatt
     3421 tgctcacatg gattaaatta aactacatac tataagatat aaacttctgc ctacagctgt
     3481 aagaaactcc gctcagtact gaagcaccag tcctatttct tcttttctcc agcctgttat
     3541 attaagcata ctgattaacg acttttaacg ttatccgcta aataaacata tttgaatgca
     3601 tgcgaccaca gtgaaaaaca aaatcacgca aagagataac tataaagaaa tcatgtctgt
     3661 aattgtggtt gtcttattac tgacacttac gttgatagcc attttttcgg caattgatca
     3721 gctgagtatt tcagaaatgg gtcgcattgc aagagatctt acacatttca ttatcaatag
     3781 tttgcaagac tggaaataaa cagcaaatta taaatgtgaa atgtttaaaa ggtatcgata
     3841 aaactttatt gttttaagga gataaaatgt cgctcgtttg ttctgttata tttattcatc
     3901 atgctttcaa cgctaacatt ttagataaag attacgcctt ctccgacggc gagatcctga
     3961 tggtagataa cgctgttcgt acacattttg aaccttatga gcggcatttt aaagaaatcg
     4021 gatttaatga aaataccatt aaaaaatatc tacaatgcac taacatccag acagtgacgg
     4081 tgcctgttcc tgcgaagttt ttacgtgctt caaatgtacc gactggattg cttaatgaaa
     4141 tgattgctta tctcaactcg gaagaacgca atcatcataa tttttcagaa cttttgcttt
     4201 tttcttgcct gtctattttt gccgcatgca aaggtttcat tacactatta actaacggtg
     4261 tgctatccgt ttccgggaaa gtgagaaata ttgtcaacat gaagctggcg cacccatgga
     4321 agctgaaaga tatttgtgac tgcctgtaca tcagtgaaag cctgttgaag aaaaaactaa
     4381 agcaagagca aacgacattc tcacagattc tattagatgc cagaatgcag cacgcaaaaa
     4441 atttgatacg cgtagaaggt tcagtcaata aaattgccga acaatgtggt tatgccagta
     4501 catcttattt tatttatgcg ttccgcaaac atttcggcaa cagtccgaag agagtttcta
     4561 aggagtaccg ttgtcaaagg cacacgggta tgaatacggg caacacgatg agtgctttag
     4621 ctatttgatt atttgctaac gagtagtcaa ccacgcacgc tacgtaagaa ttatatgggg
     4681 cagccattcc ctgccccgcg ttgtttttag gcgatatatt tattgaaata aataagtgac
     4741 atccatcaca tatttatgca cttgcataac ctgttgcatg attatttatg atctcaattc
     4801 ggcattttgt cagtaaaatg caataattta ttaaatatca ataaattagt tgtttatcgg
     4861 cgagaaatta cttaatagaa cagaaagtaa tgtcaacgct ttatggactg ttatttccct
     4921 ttttttagct aaatctgcta tctctttatg tgactaactt cacttacatc cacttatttc
     4981 tcttcgtaaa attacttcgg aattaagtac aataagaaga ggaacattta tgaagtctgc
     5041 attaaagaaa agtgtcgtaa gtacctcgat atctttgata ctggcatctg gtatggctgc
     5101 atttgctgct catgcggcag atgatgtaaa gctgaaagca accaaaacaa acgttgcttt
     5161 ctcagacttt ac
//
LOCUS       DAJTRC010000182         5141 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.184, whole genome shotgun sequence.
ACCESSION   DAJTRC010000182 DAJTRC010000000
VERSION     DAJTRC010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.184"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            180..437
                     /locus_tag="OO357_003924"
                     /pseudo
     CDS             180..437
                     /locus_tag="OO357_003924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309940.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2534 family protein"
     gene            complement(487..1437)
                     /gene="uspE"
                     /locus_tag="OO357_003925"
     CDS             complement(487..1437)
                     /gene="uspE"
                     /locus_tag="OO357_003925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein UspE"
                     /protein_id="HCR5888680.1"
                     /translation="MAMYQNMLVVIDPNQDDQPALRRAVYLHQRIGGKIKAFLPIYDF
                     SYEMTTLLSPDERTAMRQGVISQRTAWIHEQAKYYLNAGVPIEIKVVWHNRPFEAIIQ
                     EVISGGHDLVLKMAHQHDRLEAVIFTPTDWHLLRKCPSPVWMVKDQPWPEGGKALVAV
                     NLASEEPYHNALNEKLVKETIELAEQVNHTEVHLVGAYPVTPINIAIELPEFDPSVYN
                     DAIRGQHLLAMKALRQKFGINENMTHVEKGLPEEVIPDLAEHLQAGIVVLGTVGRTGI
                     SAAFLGNTAEQVIDHLRCDLLVIKPDQYQTPVELDDEEDD"
     gene            complement(1589..2341)
                     /gene="fnr"
                     /locus_tag="OO357_003926"
     CDS             complement(1589..2341)
                     /gene="fnr"
                     /locus_tag="OO357_003926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007849744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate/nitrate reduction transcriptional
                     regulator Fnr"
                     /protein_id="HCR5888681.1"
                     /translation="MIPEKRIIRRIQSGGCAIHCQDCSISQLCIPFTLNEHELDQLDN
                     IIERKKPIQKGQTLFKAGDELKSLYAIRSGTIKSYTITEQGDEQITGFHLAGDLVGFD
                     AIGSGHHPSFAQALETSMVCEIPFETLDDLSGKMPNLRQQMMRLMSGEIKGDQDMILL
                     LSKKNAEERLAAFIYNLSRRFAQRGFSPREFRLTMTRGDIGNYLGLTVETISRLLGRF
                     QKSGMLAVKGKYITIENNDALAQLAGHTRNVA"
     gene            complement(2536..3051)
                     /gene="ogt"
                     /locus_tag="OO357_003927"
     CDS             complement(2536..3051)
                     /gene="ogt"
                     /locus_tag="OO357_003927"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HCR5888682.1"
                     /translation="MLRLLEEKIATPLGPLWVICDEQFRLRAVEWEEYSERMVQLLDI
                     HYRKEGYERISATNPGGLSDKLREYFAGNLSIIDTLPTATGGTPFQREVWKTLRTIPC
                     GQVMHYGQLAEQLGRPGAARAVGAANGSNPISIVVPCHRVIGRNGTMTGYAGGVQRKE
                     WLLRHEGYLLL"
     gene            complement(3062..3205)
                     /locus_tag="OO357_003928"
                     /pseudo
     CDS             complement(3062..3205)
                     /locus_tag="OO357_003928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415852.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
     gene            complement(3595..4410)
                     /locus_tag="OO357_003929"
     CDS             complement(3595..4410)
                     /locus_tag="OO357_003929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707689.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5888683.1"
                     /translation="MTITLQPKASPGHHIIGLDSEHLNGGTVPWHKHLYAQLLYPAEG
                     VVRVWAGESVWLVHASSALWLPPQMPHKFVATGNVLLKTVLVSEAESETLGTVCFMTG
                     ISPLLRELLIAINQLPPSQSTTDKQQLRFSALETLILQEIKMGVKMSLELPWPNDERL
                     QQLCENLLNNQGYLPTLDNLADKINVSSRTLMRLFVKETGLTFRHWVQQMHVISAVTL
                     LDDGYSLTKIAHRLGYASAESFGNMFKRRTGYSPGKFTRRLTMHNYAITRQMI"
     gene            4507..>5141
                     /locus_tag="OO357_003930"
     CDS             4507..>5141
                     /locus_tag="OO357_003930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000587186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HCR5888684.1"
                     /translation="MIISKKQLIGVVAIGILLTGVVFFIWRVSKGRFIQTTDDAYIGG
                     NITTVASKVSGYISAIEVRDNQSVKKGDIILRLDDRDYRANVARLEAKIKSSKANLEG
                     IQATITMQQSIIQSASETWQAVKHEEQKRLRDTERYEKLAQSAAISQQIIDNARFDYQ
                     QVAAKERKAANDFLVEKQRLAVLSAQEENVRASIEEVQAALTQALLDLEYTL"
BASE COUNT         1372 a         1173 c         1225 g         1371 t
ORIGIN      
        1 cgcgacaatt cattcagaac atcaatagtc agggaaggaa gtagcaacat tagctaacta
       61 atcaatgcgt tgattgtaaa tccagctaag aggtgaggtt ttcagagcag acaacggtga
      121 aacgtcatgg tattgttacg tttaggtaac aagaaatttg tctgcacaag gattacatca
      181 tgattatggc gaaactgaag tcagcgaaag ggaagaaatt tctctttggt ttgttggcgg
      241 ttttcattat tgcggcgtcg gttgtgactc gcgcgaccat cggcggcgtt atagaacagt
      301 acaatattcc gctgtctgag tggacgacat caatgtatgt gatttagtca tcgatgattt
      361 ttgtttatag cctggtcttt actgtgttgc tggcaatccc gttgggaatt tatttccttg
      421 gcggcgaaga gcagtaagta aaaaataggc ccgataactc gggccttgtc agttattgaa
      481 gagtcgttaa tcgtcttctt cgtcatccag ttcaacgggt gtctgatact ggtcaggttt
      541 aatgaccagc aggtcgcagc gaagatgatc aatcacctgt tccgccgtgt tgccgaggaa
      601 tgctgctgaa ataccggtgc gtccgaccgt gcccagaacc acaattcccg cctgtaaatg
      661 ctccgccaaa tcaggaatca cctcttcggg cagacctttt tctacgtgcg tcatgttttc
      721 attaatgccg aatttctgcc gcagggcttt cattgccagc aaatgttgcc cacgaatggc
      781 atcgttataa acgctcgggt caaattccgg cagttcaatc gcgatattaa ttggcgttac
      841 cggataagcg ccaaccagat gaacttcggt atggttgact tgttctgcca gttcgatcgt
      901 ctctttgacc agtttttcat tgagcgcatt atgatacggc tcttcactgg cgagattcac
      961 cgccaccagc gccttgcctc cttccggcca cggctggtct ttcaccatcc acaccgggct
     1021 tgggcatttg cgtaacagat gccagtccgt tggcgtaaaa atcaccgctt ccagacggtc
     1081 atgttggtgc gccattttta gcaccaaatc gtgtccgccg ctgatcactt cctgaatgat
     1141 ggcttcgaaa ggacggttat gccagaccac tttaatttca atgggaacgc cagcattgag
     1201 ataatatttt gcctgctcgt ggatccaggc tgtacgctgg ctgatgacgc cctgacgcat
     1261 agcggtacgt tcgtccgggg agagcagggt ggtcatttcg tatgagaagt catagatcgg
     1321 caaaaaggct ttaattttgc caccaatccg ttgatgtaaa taaacagctc gccgcaatgc
     1381 tggttggtcg tcctggttag gatcgataac aacgagcatg ttctgataca tagccataca
     1441 gggtctcctt acaacaactg tcaacgcagt ttgtaattaa aagattaacc catatctggt
     1501 gaatgaaaca gtgatgaacc ttctgccaga tcaataaatc agaaaaattt aatgatatga
     1561 cagaaggata gtgagttatg cggaaaaatc aggcaacgtt acgcgtatgg ccagcaagct
     1621 gggccagcgc atcgttattt tcgatggtga tgtatttacc tttgactgcc agcataccgc
     1681 ttttctggaa gcgacccagc agacggctga tggtttctac cgtcaggccc agatagttac
     1741 caatatcgcc acgagtcatc gtcaggcgga attcacgagg ggagaagccg cgttgggcaa
     1801 aacgacggga caggttgtag atgaatgcag ccagacgttc ctcggcattt ttcttcgaca
     1861 acagcaagat catgtcctga tcgcctttga tttcaccgct catcagacgc atcatctgct
     1921 gacgcagatt cggcatttta ccggacaaat cgtccagcgt ttcgaacgga atttcacata
     1981 ccatcgaggt ttccagcgcc tgagcgaagc tcgggtgatg gccgctgccg atggcgtcaa
     2041 atcccactag gtcgcctgct aaatggaaac cagtgatttg ctcgtcgcct tgctcagtga
     2101 ttgtataact tttaatcgta ccggagcgga tggcataaag cgatttaagt tcatcaccag
     2161 ccttaaacag cgtctggcct ttctgaatag gcttcttccg ctcaatgata ttatcaagct
     2221 gatcaagctc atgttcgttg agtgtgaacg ggatgcaaag ctggctgatg ctgcaatcct
     2281 ggcaatggat agcacaaccg ccagactgaa tgcgccgtat aattcgcttt tccgggatca
     2341 taggtctgct caagccgtaa ttgatatttg tcaattttaa catcttttta gggagcaagt
     2401 aagtctaagc aaaccttaac agcagagaat tccgatatta gatgtaaata tatgtctatc
     2461 tatttgaaac tccttaagtt gtcaagggta actttacata aaagtgtgaa caagctggca
     2521 caaattgttt aatgtttaca gcaaaagata accttcgtgg cgcaataacc actcttttcg
     2581 ctgaactccg cctgcatatc cggtcatggt gccgtttcgg ccaataaccc gatggcaagg
     2641 tacgacgatg ctgatgggat tcgatccgtt tgccgcacca acggcacgcg ccgcgccagg
     2701 gcggcccaat tgttcagcca gttggccgta atgcattacc tgcccgcagg ggatagtgcg
     2761 tagtgttttc cagacttcgc gctgaaatgg cgtccccccc gtggcagtgg gaagcgtatc
     2821 aataatgcta agattaccgg caaaatattc acgaagcttg tcgcttaaac cacctgggtt
     2881 ggtggcagaa atgcgctcat agccttcttt gcgataatgg atgtccagca gctgcaccat
     2941 gcgttcgctg tactcttccc attcaaccgc ccgcaggcga aattgctcat cgcaaatcac
     3001 ccacagtgga cccagtggcg tggcaatttt ttcttcaagt aatctcagca tccgttctct
     3061 cttaagacaa acgtgggtaa ataccctgac ctatcggcag accgacaaga taccacgcca
     3121 gcaacatcag cagccatacc accaaaaaga taagtggata gggcaagact aacgaatagt
     3181 aagtacccag tttcgcgtct ggtttgtcgc agtctgatag cataaagcga ttttttaatt
     3241 tttctgcctg aagaaatgag ggcaagccat cacatggatc gagggtggga taattccagt
     3301 cagtgaaatt ttaccgtagg ctatctgtta tttgattctg tggcgtgata gccgccagac
     3361 accactgaac tcgtcactca ttccccaatc ccggcgagaa tgtggttatt tacagagcta
     3421 aaaaatgaat aaaaaaatag agcctgtcga catccgccag actctacagt acacacagca
     3481 gtgcatcctc gtcttaaatc cggcctgggg tcctggtgac cggtgacagt gttataacgg
     3541 ttttacccat ccgttgccaa ttttcttcag tgacagattt cacgaaaata taagttaaat
     3601 catttgtctt gtgattgcat aattatgcat cgttaatcga cgagtaaatt taccgggcga
     3661 gtagcccgtt cgacgcttaa acatattgcc gaacgattca gctgaggcat agcccagacg
     3721 atgggcaatt ttggtcaacg aatatccatc atcaagaagc gtcactgcag aaataacgtg
     3781 catttgctga acccagtggc gaaatgttaa tcccgtctct ttaacaaata agcgcatgag
     3841 tgtacggcta ctgacgttaa ttttatccgc tagattatcc agtgttggca gatacccttg
     3901 gttgtttagc aaattctcac aaagctgctg tagccgctca tcatttggcc aagggagttc
     3961 aagtgacatt ttcaccccca tttttatttc ctgaagtatc agtgtctcga gggctgaaaa
     4021 acgtaactgt tgtttatctg tggtactttg cgacggtgga agttggttaa ttgcaattag
     4081 taattccctt aacaggggtg aaatacccgt catgaagcaa actgttccca gcgtttcact
     4141 ttccgcttct gaaaccagta cggtttttaa aagtacgttg cctgtcgcaa caaacttatg
     4201 cggcatttgt gggggcagcc aaagcgcgct gctggcatgg actagccaga cgctttctcc
     4261 agcccataca cgaacgactc cttcagccgg gtaaagcaac tgggcataga gatgtttatg
     4321 ccagggaact gtacctccat tcagatgttc cgaatccagt ccgataatgt gatgccctgg
     4381 tgatgctttg ggttgcagtg taattgtcat ttacagtagg tattttgtca gaaatcatca
     4441 aataagccat gttaaatttt aaatatgacg tatgcaagtt tccggatata tacggtgtaa
     4501 agagtgatga taatttctaa aaaacagctt attggggtgg tagcgatcgg tattcttttg
     4561 actggcgttg tcttttttat ctggagggta agcaaaggaa gatttataca gacgacggat
     4621 gatgcttaca ttggtggtaa tatcaccacg gtggcaagta aagtttctgg ttatatttct
     4681 gctatagagg tgcgcgataa tcagagcgta aaaaaaggcg atattatcct gcgtctcgat
     4741 gatcgggatt atcgtgcaaa tgtcgccagg cttgaggcaa aaataaaaag tagcaaagcc
     4801 aatctcgaag gcattcaagc cactattacg atgcagcagt cgataattca aagtgccagt
     4861 gaaacatggc aggcagtaaa acatgaggag caaaagcgcc tgcgagatac tgaacgttat
     4921 gaaaaactcg cacaatcagc ggcaatatcg caacaaatta ttgataacgc caggtttgac
     4981 taccagcagg tcgcagcaaa ggagcgcaaa gccgcgaacg atttcctggt ggagaagcaa
     5041 cggctggcag ttttatctgc tcaggaagaa aacgtacgtg cgagtatcga agaagttcag
     5101 gccgctttga ctcaggcact actggatctg gaatataccc t
//
LOCUS       DAJTRC010000183         5104 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.185, whole genome shotgun sequence.
ACCESSION   DAJTRC010000183 DAJTRC010000000
VERSION     DAJTRC010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5104
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.185"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(327..572)
                     /locus_tag="OO357_003931"
     CDS             complement(327..572)
                     /locus_tag="OO357_003931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000875412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888685.1"
                     /translation="MKYKEVACGKQRMHWLALQFVDENNNPVSGLNVQLEYHPRAMAA
                     ELALWARGRHTQFDPTPPPPPVGVTDSQGLVRFDDLY"
     gene            complement(1349..2191)
                     /locus_tag="OO357_003932"
     CDS             complement(1349..2191)
                     /locus_tag="OO357_003932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
                     /protein_id="HCR5888686.1"
                     /translation="MSEITASRPEVVNGHTDVICSTSIRHILAVRKSTLLQIDTLIRQ
                     LAEVSAMTESIRGKTALDWAMKQDFRCGCWLMEKPETAMKAITRNLDRELWRDLMQRS
                     GMLSLMDAQTRDTWYRSLEYDNFPEISEANILSTFEQLHQNKDEVFERGVINVFKGLS
                     WDYKTNSPCKFGSKIIVNNLVRWDRWGFHLITGQQTDRLVDLERMLHLFSGKPIPDNR
                     ENITIRLDGHIQSVQGKERYEDEMFIIKYFKKGSAHITFKRLELIDRINDIIARYFPS
                     VLSA"
     gene            complement(2276..2473)
                     /locus_tag="OO357_003933"
     CDS             complement(2276..2473)
                     /locus_tag="OO357_003933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888687.1"
                     /translation="MKLLTTETALDILIAWLQDNIDCESGIIFDNDEDKTDSAALLPC
                     IEQAREDIRTLRQLQFLQQNR"
     gene            complement(2493..2981)
                     /locus_tag="OO357_003934"
     CDS             complement(2493..2981)
                     /locus_tag="OO357_003934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888688.1"
                     /translation="MKLALTLEADSVNVQALNMGRIVVDVDGVNLSELINKVSENGYS
                     LRVVDKSDQHATSTPPPLTTLTCIRCSTAHITETDNAWLYSLSHQTNDDGETEWIHFT
                     GSGYLLRTDAWSYPVLRLKRLGLSRTFRRLVVTLTRRYGVSLIHLDAGAECLPGFPTF
                     DW"
     gene            complement(2978..3355)
                     /locus_tag="OO357_003935"
     CDS             complement(2978..3355)
                     /locus_tag="OO357_003935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5888689.1"
                     /translation="MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFA
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSAGAPSQLINSIDILRARRATG
                     LMTRDNYRTVNNITRGKHPEAKQ"
     gene            complement(3402..3779)
                     /gene="cbtA"
                     /locus_tag="OO357_003936"
     CDS             complement(3402..3779)
                     /gene="cbtA"
                     /locus_tag="OO357_003936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system toxin CbtA"
                     /protein_id="HCR5888690.1"
                     /translation="MSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYL
                     ADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGGELNPRHQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAAPATTV"
     gene            complement(3859..4080)
                     /locus_tag="OO357_003937"
     CDS             complement(3859..4080)
                     /locus_tag="OO357_003937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5888691.1"
                     /translation="MKIITRGEAMRIHRQHPASRLFPFCTGKYRWHGSTDTYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            complement(4167..4643)
                     /locus_tag="OO357_003938"
     CDS             complement(4167..4643)
                     /locus_tag="OO357_003938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RadC family protein"
                     /protein_id="HCR5888692.1"
                     /translation="MQQLSFLPGEMTTRERSLILRALKTLDRHLHEPGVAFTSTHAAR
                     EWLILNMAGLEREEFRVLYLNNQNQLIAGETLFTGTINRTEVHPREVIKRALYHNAAA
                     VVLAHNHPSGEVPPSKADRLITERLVQALALVDIRVPDHLIVGGSQVFSFAEHGLL"
     gene            complement(4659..>5104)
                     /locus_tag="OO357_003939"
     CDS             complement(4659..>5104)
                     /locus_tag="OO357_003939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708769.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="antirestriction protein"
                     /protein_id="HCR5888693.1"
                     /translation="SACAPETDLQQLVATLVPDEQRISFWPQHFGLIPQWVTLEPRVF
                     GWMDRLCEDYCGGIWNLYTLNNGGAFMAPEPDDDDDETWVLFNAMNGNRAEMSPEAAG
                     IAACLMTYSHHACRTECYAMTVHYYRLRDYALQHPECSAIMRIID"
BASE COUNT         1190 a         1242 c         1351 g         1321 t
ORIGIN      
        1 ccccagaaaa gaagacaggc atagagtttt gtgatctact gattgcgcta acaaattcaa
       61 tgagatcacc tctatgcctg cccgttaagt atgtcaggct tttttccgta atgctctaac
      121 ttccacgcat tggtttctga catcaaataa ggctatgact ttctctccct tttaatctca
      181 ttctgatcta aaaccatatc ttaaaaaaat gcatgggtat tttaattatt ttgatgttga
      241 tctaacgcac tcaaggacca ttttattacc gattatccat tctatttaac tgttgcgata
      301 taaatgtttg ttttaggtgc tttaattcag tacaagtcat caaaccgtac cagaccctga
      361 ctgtcagtga caccgacagg tgggggcggc ggtgtggggt caaactgagt gtgtctgcct
      421 ctggcccaca gagccagctc tgccgccatt gcccgtggat gatactccag ctgcacgttg
      481 agaccggaaa ctgggttgtt gttctcatcc acgaactgca gcgccagcca gtgcatccgc
      541 tgcttaccgc aggcgacttc tttgtacttc attttgtgac tcaggcaggt gactgtcgtg
      601 ttgcctgttg cgtccatttt gcggttaaac ccttttaatt caatttgtta aattgctttc
      661 caccttttgc ggggcgcgac ttaaccaaaa tattcactgt tatcataata tgcaatgggc
      721 tggaggttgc aggtcattta gggattttct gtaagccccg ttttgcagaa cgttctatct
      781 ccgattgaga tcctgtctct cggtattacc ggtagtctgg ccataatccc ctatggatca
      841 tattgatata gtaggcataa cacccaaaag ttgactaaag ctaaatcaat cagagtgaaa
      901 aatgttcggt ttgatgttgg gggggactga ggaggggcca acgcaggtgg tatgcgtttt
      961 acttcagacc ggacaaagac cggatgtatc cgtgcagtaa tgaatccgtt agccaggtgc
     1021 tcatgtggaa atagtgtcta ttggggattt gaagtttgac agaaaaggcc acagtgacca
     1081 gaagggggct acttagaaga aatgttcagg ctgggtggaa gaggtaaagc agtgacatca
     1141 gcattcaaag tgaccggata tctaccagaa ccccagaaag caaaaaacca gcccgtaggc
     1201 tggttcttct aaatagtggt gccggactcg gaatcgaaaa tttcttttat catattgata
     1261 ttaatgcaat ttgtttggtt tgattaaaaa tgtaccctca attatacccg cagtcccttt
     1321 tgatatcgaa agggaatcac tctcgggatc aggctgagag cacagaagga aagtacctgg
     1381 ctattatatc gttaattctg tcaatcagtt ccagcctttt gaaagtgatg tgtgcagatc
     1441 ccttcttaaa gtatttaatg atgaacatct cgtcttcata gcgctcttta ccctgaacgg
     1501 actggatgtg accatccaga cgaatagtga tgttttcccg gttgtcgggg atcggtttgc
     1561 cgctgaacag atgtagcatt ctttccaggt cgacgagtcg gtcagtctgt tgcccggtga
     1621 taagatgaaa cccccaccgg tcccacctca ccaggttgtt gacgataatt ttactgccaa
     1681 atttacaggg tgagttggtt ttgtaatccc agctcagccc tttgaagacg ttgatcactc
     1741 cccgttcaaa cacttcatct ttgttctggt gcagttgttc aaatgtgctc agaatgttcg
     1801 cttcgctgat ttccggaaaa ttatcatatt ccagtgacct gtaccatgtg tcccgggtct
     1861 gcgcatccat cagactcagc atcccggaac gttgcatcag atcacgccag agttcgcgat
     1921 caagattgcg tgtgatggct ttcattgcgg tttccggttt ctccatcagc cagcaaccgc
     1981 agcgaaaatc ctgtttcatg gcccagtcca gagccgtttt acctctaata ctttctgtca
     2041 ttgctgatac ttcagccagt tgtcggataa gtgtgtcgat ttgcagcaat gtactctttc
     2101 gtacagccag aatgtggcgg atactggttg agcagataac gtccgtatgc ccgttgacca
     2161 cttccggacg agaggctgtg atttcagaca taatcgatat tcctttttgc agataaacga
     2221 aaacgccagc cgtattcagg ctggcgtcag tggaatgaag tctggtgagt aagactcacc
     2281 ggttctgttg cagaaactgc agctgacgca gggtacggat atcttccctg gcctgttcaa
     2341 tacagggcag cagtgctgcc gaatccgttt tatcctcatc gttgtcgaag ataatccctg
     2401 attcgcagtc aatattgtcc tgcagccacg caatcagaat atccagcgcc gtttctgtgg
     2461 ttaataattt catccagtgt cttttgtcct gattaccagt cgaaagtggg gaaccccggc
     2521 agacattctg cgccggcgtc cagatgaatg agactgacgc cataacgccg ggtgagagtg
     2581 acgaccagac gacggaacgt tctggacagg cccaggcgtt taagccgcag aaccgggtac
     2641 gaccatgcat cggtacgtaa cagatagccg ctgcctgtaa aatgaatcca ttctgtttcg
     2701 ccgtcgtcat tagtctggtg tgacagcgag tacagccagg cgttgtccgt ttccgtgata
     2761 tgtgcggtgc tgcaacgtat gcaggtaagg gttgttaacg gtggtggtgt actggttgcg
     2821 tgttggtcgg atttatcgac cacgcggagt gaataaccgt tttcagagac cttgttaatc
     2881 agttcactga gattaacacc atcgacgtca acgacaatgc gccccatgtt cagtgcctgc
     2941 acgttaacgc tgtcggcttc cagcgtcagg gccagtttca ttgtttcgct tccggatgct
     3001 tcccccgggt aatgttattt accgttctgt agttatcgcg ggtcatcagg ccggttgccc
     3061 ggcgtgcccg gagaatatca atgctgttga ttaactgcga cggggctcct gcgctgaatc
     3121 ccggctggtc ggtacgtacc agcgcgtatt tttcaacgag aaagttcacc gcgtcacaca
     3181 gtgaaattcc tgcctcaata tgctgctcaa tcacacgttc atcagcgaac ggtgtgtcat
     3241 tcagcgtgag gccatagtgc tggtccagca gtcgggtgag cagtgtctgc cagatggtga
     3301 tgggagacgg gcagcccgat gcctcccgta cgtgagtgtc gggtaatgta ttcatcttaa
     3361 gttctctgta acaggtggtt ataaagggga gatatccggt gttatacggt ggttgcgggc
     3421 gctgccggtg tcggataaac agccagataa acgtaaccac aggagccgag ggtgtcggct
     3481 tcgcaggtca gccctttcgc atacagtgtg acggtatgct gatggcgggg attcagttca
     3541 ccgccagtga gcatgagttc cagttgtttc atcagcagcg gaaaggcctg gtccaggtgg
     3601 tacgcatcta cgtcgctgaa cctgcctctg ataccggcgc ggtctgcaag gtaatgcagg
     3661 cggttaccct cctgtaccag tcgggccccg aaacagggcg tcacggtgca gggcagcccc
     3721 caccaggggc ggtcgtgatt gtcgtcggga ggcgttgtcc cggggagtgt gtctgacacg
     3781 ataaagtcct cacagaaaat cggttaagaa tggtccggta gtggcgataa ttctgctcat
     3841 cagaattccc ttccagactc agttcagggt gacgctcatc agccggacat acggaccaaa
     3901 actgtcctta cggcgttctg caaacacagc cagtacaccg ggaatatcct gcacttcacg
     3961 gccggtatat gtatccgtgc taccgtgcca gcggtattta ccggtacaga acggaaaaag
     4021 acgggatgca ggatgctgac ggtgaatacg catggcttca ccacgggtga tgattttcat
     4081 gagaatagtc ctcctgaatg tgaaagtaag aaacagctga gaaagagaaa ctgaagtgat
     4141 atgaaggtga ttgtgacggt gacgggttaa agcagaccgt gttccgcaaa ggagaaaacc
     4201 tggctgccac cgactatcag atggtccggt acccggatat caaccagggc cagtgcctgt
     4261 accagacgct cagtgataag gcggtctgcc ttgctggggg ggacttcacc ggacgggtga
     4321 ttgtgcgcca gcaccacggc agcggcattg tggtacaggg cgcgtttaat cacttcccgg
     4381 ggatggactt ccgtacggtt gatggtgccg gtgaagaggg tttcaccggc aatcagctga
     4441 ttctggttgt tcagatacag cacccggaac tcttcacgct ccagtcccgc catgttcaga
     4501 atcagccatt cccgtgccgc atgcgtggag gtgaaggcca cgccgggttc atgaagatgg
     4561 cggtccaggg ttttcagggc ccgcagaatg aggctgcgct ctctggttgt catttctccg
     4621 ggcagaaagg aaagctgctg cattgtccct gccaccattc agtcgatgat gcgcataatg
     4681 gcgctgcatt ccggatgctg cagggcgtaa tcccgcagcc ggtaataatg gaccgtcatg
     4741 gcataacact ccgtacgaca ggcatgatga ctgtacgtca tcagacaggc agcaatgccg
     4801 gcagcttccg gactcatttc agcgcggtta ccgttcatgg cattgaacag tacccatgtt
     4861 tcgtcatcat cgtcatccgg ttcgggtgcc ataaatgccc cgccgttgtt cagggtgtac
     4921 agattccaga taccaccgca gtagtcttcg cacagacggt ccatccagcc gaagacacgg
     4981 ggctccaggg tcacccactg tggaatgagg ccaaagtgct gcggccagaa gctgatgcgc
     5041 tgttcatcag ggacgagggt ggcaaccagt tgttgcaggt cagtctccgg tgcacaggcc
     5101 gaag
//
LOCUS       DAJTRC010000184         5025 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.186, whole genome shotgun sequence.
ACCESSION   DAJTRC010000184 DAJTRC010000000
VERSION     DAJTRC010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.186"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            48..122
                     /gene="tnaC"
                     /locus_tag="OO357_003940"
     CDS             48..122
                     /gene="tnaC"
                     /locus_tag="OO357_003940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase leader peptide"
                     /protein_id="HCR5888694.1"
                     /translation="MNILHVCVTSKWFNIDNKIVDHRP"
     gene            343..1758
                     /gene="tnaA"
                     /locus_tag="OO357_003941"
     CDS             343..1758
                     /gene="tnaA"
                     /locus_tag="OO357_003941"
                     /EC_number="4.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709490.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophanase"
                     /protein_id="HCR5888695.1"
                     /translation="MENFKHLPEPFRIRVIEPVKRTTRAYREEAIIKSGMNPFLLDSE
                     DVFIDLLTDSGTGAVTQSMQAAMMRGDEAYSGSRSYYALAESVKNIFGYQYTIPTHQG
                     RGAEQIYIPVLIKKREQEKGLDRSKMVAFSNYFFDTTQGHSQINGCTVRNVYIKEAFD
                     TGVRYDFKGNFDLEGLERGIEEVGPNNVPYIVATITSNSAGGQPVSLANLKAMYSIAK
                     KYDIPVVMDSARFAENAYFIKQREAEYKDWTIEQITRETYKYADMLAMSAKKDAMVPM
                     GGLLCMKDDSFFDVYTECRTLCVVQEGFPTYGGLEGGAMERLAVGLYDGMNLDWLAYR
                     IAQVQYLVDGLEEIGVVCQQAGGHAAFVDAGKLLPHIPADQFPAQALACELYKVAGIR
                     AVEIGSFLLGRDPKTGKQLPCPAELLRLTIPRATYTQTHMDFIIEAFKHVKENAANIK
                     GLTFTYEPKVLRHFTAKLKEV"
     gene            1849..3096
                     /gene="tnaB"
                     /locus_tag="OO357_003942"
     CDS             1849..3096
                     /gene="tnaB"
                     /locus_tag="OO357_003942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low affinity tryptophan permease TnaB"
                     /protein_id="HCR5888696.1"
                     /translation="MTDQAEKKHSAFWGVMVIAGTVIGGGMFALPVDLAGAWFFWGAF
                     ILIIAWFSMLHSGLLLLEANLNYPVGSSFNTITKDLIGNTWNIISGITVAFVLYILTY
                     AYISANGAIISETISMNLGYHANPRIVGICTAIFVASVLWISSLAASRITSLFLGLKI
                     ISFVIVFGSFFFQVDYSILRDATSTTAGTSYFPYIFMALPVCLASFGFHGNIPSLIIC
                     YGKRKDKLIKSVVFGSLLALVIYLFWLYCTMGNIPRESFKAIISSGGNIDSLVKSFLG
                     TKQHGIIEFCLLVFSNLAVASSFFGVTLGLFDYLADLFKIDNSHGGRFKTVLLTFLPP
                     ALLYLIFPNGFIYGIGGAGLCATIWAVIIPAVLAIKARKKFPNQMFTVWGGNLIPAIV
                     ILFGITVILCWFGNVFNVLPKFG"
     gene            3228..4403
                     /gene="mdtL"
                     /locus_tag="OO357_003943"
     CDS             3228..4403
                     /gene="mdtL"
                     /locus_tag="OO357_003943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter MdtL"
                     /protein_id="HCR5888697.1"
                     /translation="MSRFLICSFALVLLYPAGIDMYLVGLPRIAADLNASEAQLHIAF
                     SVYLAGMAAAMLFAGKVADRSGRKPVAIPGAALFIIASVFCSLAETSTLFLAGRFLQG
                     LGAGCCYVVAFAILRDTLDDRRRAKVLSLLNGITCIIPVLAPVLGHLIMLKFPWQSLF
                     WAMAMMGIAVLMLSLFILKETRPASPAASDKPRENSESLLNRFFLSRVVITTLSVSVI
                     LTFVNTSPVLLMEIMGFERGEYATIMALTAGVSMTFSFSTPFALGIFKPRTLMITSQV
                     LFLAAGITLAVSPSHAVSLFGITLICAGFSVGFGVAMSQALGPFSLRAGVASSTLGIA
                     QVCGSSLWIWLAAVVGIGAWNMLIGILIACSIVSLLLIMFVAPGRPVAAHEEIHHHA"
     gene            4378..>5025
                     /gene="yidZ"
                     /locus_tag="OO357_003944"
     CDS             4378..>5025
                     /gene="yidZ"
                     /locus_tag="OO357_003944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH-type transcriptional regulator YidZ"
                     /protein_id="HCR5888698.1"
                     /translation="MKKSITTLDLNLLLCLQLLMQERSVTKAAKRMNVTPSAVSKSLA
                     KLRAWFDDPLFVNSPLGLSPTQLMVSMEQNLAEWMQMSNLLLDKPHHQTPRGLKFELA
                     AESPLMMIMLNALSKRIYQRYPQATIKLRNWDYDSLDAITRGEVDIGFSGRESHPRSR
                     ELLSSLPLAIDYEVLFSDVPCVWLRKDHPALHETWNLDTFLRYPHISICWEQSDTW"
BASE COUNT         1144 a         1208 c         1257 g         1416 t
ORIGIN      
        1 tctgagtgta ataatgtagc ggcgtgtctt tcgaggataa gtgcattatg aatatcttac
       61 atgtatgtgt gacctcaaaa tggttcaata tcgacaataa gattgtcgat caccgccctt
      121 aatttaccct tctgtagcca tcaccagagc caaaccgatt agattcaatg tgatcgatta
      181 gtttgctata tcttaatttt gccttttgca aaggccatct ctcgtttatt tacttatttt
      241 agtaaatgat ggtgcttgta tatatacatg gtgaattaat ggatattgca gatgtaatat
      301 tcacagggat cactgtaatt aaaataaatg aaggattatg taatggaaaa ctttaaacat
      361 ctccctgaac cgttccgcat tcgtgttatt gagccagtaa aacgtactac tcgcgcttat
      421 cgtgaagaag caattattaa atccggtatg aacccgttcc tgctggatag cgaagatgtg
      481 tttatcgatt tactgaccga cagcggcacc ggggcggtaa cccaaagtat gcaggcagcg
      541 atgatgcgcg gcgacgaagc ctacagcggc agccgcagct actatgcgtt agccgagtca
      601 gtgaaaaata tctttggtta tcaatatact attccgactc accagggccg tggcgcagag
      661 caaatctata ttccggtact gattaaaaaa cgcgagcagg aaaaaggcct ggatcgcagc
      721 aaaatggtgg cattctctaa ctatttcttt gataccacgc agggccatag ccagattaac
      781 ggctgtaccg tgcgtaacgt ctatatcaaa gaagccttcg atacgggcgt gcgttacgac
      841 tttaaaggca actttgacct cgaaggatta gaacgcggta ttgaagaagt tggcccgaat
      901 aacgtgccgt atatcgttgc aaccatcacc agtaactctg caggtggtca gccggtttca
      961 ctggcaaact taaaagcgat gtacagcatc gcgaagaaat acgatattcc ggtggtaatg
     1021 gactccgcac gctttgctga aaacgcctat ttcatcaagc agcgtgaagc agaatacaaa
     1081 gactggacca tcgagcagat cacccgcgaa acctacaaat atgccgatat gctggcgatg
     1141 tccgccaaga aagatgcgat ggtgccgatg ggcggcttgc tgtgcatgaa agacgacagc
     1201 ttctttgatg tgtacaccga gtgcagaacc ctttgcgtgg tgcaggaagg cttcccgaca
     1261 tatggcggct tggaaggcgg cgcgatggag cgtctggcgg taggtctgta tgacggcatg
     1321 aatctcgact ggctggctta tcgtatcgcg caggtacagt atctggtcga tggtctggaa
     1381 gagattggcg ttgtctgcca gcaggcgggc ggtcacgcgg cattcgttga tgccggtaaa
     1441 ctgctgccgc atatcccggc agaccagttc ccggcacagg cgctggcgtg cgagctgtat
     1501 aaagtcgccg gtatccgtgc ggtagaaatt ggctctttcc tgttaggccg cgatccgaaa
     1561 accggtaaac aactgccatg cccggctgaa ctgctgcgtt taaccattcc gcgcgcaaca
     1621 tatactcaaa cacatatgga cttcattatt gaagccttta aacatgtaaa agagaacgcg
     1681 gcgaatatta aaggattaac ctttacctac gaaccaaaag tattgcgtca cttcaccgca
     1741 aaactgaaag aagtttaatt aatactacag agtggctata aggatgttag ccactctctt
     1801 accctacatc ctcaataaca aaaatagcct tcctctaaag gtggcatcat gactgatcaa
     1861 gctgaaaaaa agcactctgc attttggggt gttatggtta tagcaggtac agtaattggt
     1921 ggaggtatgt ttgctttacc tgttgatctt gccggtgcct ggtttttctg gggtgccttt
     1981 atccttatca ttgcctggtt ttcaatgctt cattccgggt tattgttatt agaagcaaat
     2041 ttaaattatc ctgtcggctc cagttttaac accatcacca aagatttaat aggtaacacc
     2101 tggaacatta tcagcggtat taccgttgcc ttcgttctct atatcctcac ttatgcctat
     2161 atctctgcta atggtgcgat cattagtgaa acgatatcaa tgaatttggg ctatcacgct
     2221 aatccacgta ttgtcgggat ctgcacagcc attttcgttg ccagcgtatt gtggataagt
     2281 tcgttagccg ccagtcgcat tacctcattg ttcctcgggc tgaagattat ctcctttgtg
     2341 atcgtgtttg gttctttctt cttccaggtc gattactcca tcctgcgcga tgccaccagc
     2401 accactgcgg gaacgtctta cttcccgtat atctttatgg ctttgccggt gtgtctggcg
     2461 tcatttggtt tccacggcaa tattcccagc ctgattattt gctatggcaa acgcaaagat
     2521 aagttaatca aaagcgtggt atttggttcg ctgctggcgc tggtgattta tctcttctgg
     2581 ctctattgca cgatggggaa tattccgcgc gaaagcttta aggcgataat ctcctcaggc
     2641 ggcaacattg attcgctggt gaaatcgttc ctcggcacca aacagcacgg cattatcgag
     2701 ttttgcctgc tggtgttctc taacttagct gttgccagtt cgttctttgg tgtcacgctg
     2761 gggttgttcg attatctggc ggacctgttt aagattgata actcccacgg cgggcgtttc
     2821 aaaaccgtgc tgttaacctt cctgccacct gcgttgttgt atctgatctt cccgaacggc
     2881 tttatttacg ggatcggcgg tgccgggctg tgcgccacca tctgggcggt cattattccc
     2941 gcagtgcttg caatcaaagc tcgcaagaag tttcccaatc agatgttcac ggtctggggc
     3001 ggcaatctta ttccggcgat tgtcattctc tttggtataa ccgtgatttt gtgctggttc
     3061 ggcaacgtct ttaacgtgtt acctaaattt ggctaaatcc ttcaagaagc cagccattcg
     3121 ctggcttctt gcctctcagg aaatcactta tgtccaaatg gcaactcgcc ttatcctcct
     3181 tcaccacgta tgctttgcgt caccttacta tcaggacgct ttagcccatg tcccgctttt
     3241 tgatttgtag ttttgccctg gttttacttt atcccgccgg gattgatatg tacctcgttg
     3301 gtttaccgcg catcgccgcc gatctcaatg ccagcgaagc gcagttgcat attgcgttct
     3361 ccgtatatct ggcggggatg gcagctgcga tgctatttgc cggtaaagtg gccgatcgtt
     3421 cagggagaaa gccggtcgcc atacccggcg cggcgctatt tattattgcc tcggtgttct
     3481 gttcactggc tgaaaccagc acgttatttc ttgcaggccg atttctacag gggttgggcg
     3541 caggctgttg ttacgtggtg gcgttcgcca ttttgcgcga cacgctggat gatcgacgtc
     3601 gggctaaagt gctgtcatta ctcaacggta ttacctgcat cattccggtg ttagcgccag
     3661 tgctcggaca tctgattatg cttaaattcc cgtggcagag tctgttctgg gcgatggcaa
     3721 tgatgggcat cgcggtactg atgttgtctt tgtttatttt aaaagaaacg cgcccagcgt
     3781 cccccgctgc ttcggacaaa ccacgagaaa atagcgagtc gctgcttaat cgttttttcc
     3841 tcagccgtgt tgttatcacc accctcagcg tttcggtgat cctcactttc gtcaatacat
     3901 cgccggtatt gctgatggaa atcatggggt ttgagcgcgg agaatacgcc accattatgg
     3961 cgctgaccgc tggcgtcagc atgacctttt cattctcgac gccatttgcg ctgggaattt
     4021 ttaagccacg tacgttgatg atcacctcgc aggtgttatt ccttgcggcg gggatcaccc
     4081 ttgccgtttc accttcccat gcggtttctc tgtttggtat cacgctgatt tgcgccggtt
     4141 tctcggtagg ttttggcgta gcgatgagtc aggcgttagg accattttca ttacgcgcgg
     4201 gcgtagccag ctcgacctta ggtattgcgc aggtttgcgg ttcgtcactg tggatttggc
     4261 tggccgcggt ggttggtatc ggcgcatgga atatgctgat cgggattctg attgcctgta
     4321 gcatagtgag cctgttgctg attatgttcg tcgcgcctgg acgccccgtt gccgctcatg
     4381 aagaaatcca tcaccacgct tgatctcaat ctgctgctct gtctgcaact gctgatgcag
     4441 gagcgcagcg tgaccaaagc ggcgaagcgg atgaacgtga ccccttcagc ggtgagtaag
     4501 tcgctggcaa agttaagagc gtggtttgac gacccgctct ttgtgaactc accgctgggg
     4561 ctgtcgccca cacagctaat ggtcagcatg gagcaaaatc tggcggaatg gatgcaaatg
     4621 agcaatctgc tgctggataa accgcaccac cagacaccgc gcggcctgaa gtttgagttg
     4681 gcggcggaat cgccgctgat gatgatcatg cttaatgcgc tgtcgaaacg gatctaccaa
     4741 cgttacccgc aggcgaccat caaactacgt aactgggatt acgattcctt agatgccatt
     4801 actcgtggtg aagtggatat tggtttttcc ggtcgcgaaa gccatcctcg ctcgcgggag
     4861 ctgttaagct cgctaccgtt agccattgat tatgaagtgc tgtttagtga tgtaccctgc
     4921 gtctggttac gcaaagatca tccggcactg catgaaacgt ggaatctgga caccttttta
     4981 cgttatccgc atatcagcat ttgctgggaa cagagcgaca cctgg
//
LOCUS       DAJTRC010000185         4868 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.187, whole genome shotgun sequence.
ACCESSION   DAJTRC010000185 DAJTRC010000000
VERSION     DAJTRC010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.187"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(97..426)
                     /locus_tag="OO357_003945"
     CDS             complement(97..426)
                     /locus_tag="OO357_003945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707676.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbL family protein"
                     /protein_id="HCR5888699.1"
                     /translation="MMKKTLLLCAFLVGLVSSNVMALTLDEARTQGRVGETFYGYLVA
                     LKTDAETEKLVTEINAERKASYQQRAKQNNVSVDDIAKLAGQKLVARAKPGEYVQGIN
                     GKWVRKF"
     gene            complement(431..616)
                     /locus_tag="OO357_003946"
     CDS             complement(431..616)
                     /locus_tag="OO357_003946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnbE family lipoprotein"
                     /protein_id="HCR5888700.1"
                     /translation="MKILLAALTSSFMLVGCTPRIEVAAPKEPITINMNVKIEHEIII
                     KADKDVEELLETRSDLF"
     gene            complement(613..3252)
                     /locus_tag="OO357_003947"
     CDS             complement(613..3252)
                     /locus_tag="OO357_003947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbH family protein"
                     /protein_id="HCR5888701.1"
                     /translation="MLGKYKAVLALLLLIILVPLTLLMTLGLWVPTLADIWLPLGTRI
                     ALDESPRITRKGLIIPDLRYLVGDCQLAHITNASLSHPSRWLLNVGTVELDSACLAKL
                     PQTEQSPAAPKTLAQWQSMLPNTWINIDKLIFSPWQEWQGKLSLALTSDIQQLRYQGE
                     KVKFQGQLKGQQLTVSELDVVAFENQPPVKLVGEFTMPLVPDGLPVSGHATATLNLPQ
                     EPSLVDAELDWQENSGQLIVLARDNGDPLLDLPWQITRQQLTVSDGRWSWLYAGFPLS
                     GRLGVKVDNWQAGLENALVSGRLNVLTQGQAGKGNAVLNFGPGKLSMDNSQLPLQLTG
                     EAKQADLILYARLPAQLSGSLSDPTLTFEPGALLRSKGRVIDSLDIDEIRWPLAGVKV
                     TQRGVDGRLQAILQAHENELGDFVLHMDGLANDFLPDAGRWQWRYWGKGSFTPMNATW
                     DVAGKGEWHDSTITLTDLSTGFDQLQYGTMTVEKPRLILDKPVVWVRDAQHPSFSGAL
                     SLDAGQTLFTGGSVLPPSTLKFSVDGRNPTYFLFKGDLHAGEIGPVRVNGRWDGIRLR
                     GNAWWPKQSLTVFQPLVPPDWKMNLRDGELYAQVAFSAAPEQGFRAGGHGVLKGGSAW
                     MPDNQVNGVDFVLPFRFADGAWHLGTRGPVTLRIAEVINLVTAKNITADLQGRYPWTE
                     EEPLLLTDVSVDVLGGNVLMKQLRMPQHDPALLRLNNLSSSELVSAVNPKQFAMSGAF
                     SGALPLWLNNEKWIVKDGWLANSGLMTLRLDKDTADAVVKDNMTAGSAINWLRYMEIS
                     RSSTKINLDNLGLLTMQANITGTSRVDGKSGTVNLNYHHEENIFTLWRSLRFGDNLQA
                     WLEQNARLPGNDCPQGKECEEKQ"
     gene            3460..4449
                     /gene="ldhA"
                     /locus_tag="OO357_003948"
     CDS             3460..4449
                     /gene="ldhA"
                     /locus_tag="OO357_003948"
                     /EC_number="1.1.1.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase"
                     /protein_id="HCR5888702.1"
                     /translation="MKLAVYSTKQYDKKYLQQVNESFGFELEFFDFLLTEKTAKTANG
                     CEAVCIFVNDDGSHPVLEELKKHGVKYIALRCAGFNNVDLDAAKELGLKVVRVPAYDP
                     EAVAEHAIGMMMTLNRRIHRAYQRTRDANFSLEGLTGFTMYGKTAGVIGTGKIGVAML
                     RILKGFGMRLLAFDPYPSAAALELGVEYVDLPTLFSESDVISLHCPLTPENYHLLNEA
                     AFDQMKNGVMIVNTSRGALIDSQAAIEALKNQKIGSLGMDVYENERDLFFEDKSNDVI
                     QDDVFRRLSACHNVLFTGHQAFLTAEALTSISQTTLQNLSNLEKGETCPNELV"
     gene            4560..>4868
                     /gene="hslJ"
                     /locus_tag="OO357_003949"
     CDS             4560..>4868
                     /gene="hslJ"
                     /locus_tag="OO357_003949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein HslJ"
                     /protein_id="HCR5888703.1"
                     /translation="MKKVAAFVALSLLMAGCVSNDKIAVTPEQLQHHRFVLESVNGKP
                     VTSDKNPPEISFGEKMMISGSMCNRFSGEGKLSNGELTAKGLAMTRMMCANPQLNELD
                     N"
BASE COUNT         1231 a         1265 c         1131 g         1241 t
ORIGIN      
        1 gcaatttgac aggagttttt gaatattcac cttgtttcag gaaaatagat caatcgcttt
       61 ctcctgtagc gttatcccga tattgcggtt ttgctattaa aactttcgca cccatttacc
      121 gtttatccct tgtacatatt cccccggttt ggcccgagct acgagctttt gcccggcgag
      181 tttcgcgata tcatctacag acacattatt ttgctttgcc cgttgttggt aactcgcttt
      241 acgttcggca ttaatttcgg ttactaattt ctctgtttca gcatccgttt tcagcgcaac
      301 cagataacca taaaatgttt cacctacccg cccctgagtt ctggcttcat ccagagtcaa
      361 tgccattaca ttgctgctta ccagcccaac aagaaacgca caaagaagta atgttttctt
      421 catcatcgcc tcagaaaaga tcgctacgag tttcaagcag ctcttcgaca tctttgtctg
      481 ccttgatgat gatctcatgc tcaattttaa cgttcatatt gatagtgatc ggttccttag
      541 gtgcagcgac ttcaatgcga ggcgtacagc caaccagcat aaaagatgac gtcaacgcag
      601 ccagtaaaat tttcattgtt tttcctcaca ctcttttcct tgcggacagt catttcccgg
      661 cagacgtgcg ttctgctcca accatgcctg gagattatcg ccaaagcgta aactgcgcca
      721 cagcgtaaaa atattctctt catgatggta attaaggttt accgtaccgc ttttaccatc
      781 aacgcgactg gtacctgtaa tgttggcctg catggttaat aaaccgagat tatctaagtt
      841 aatttttgtc gatgaccggc taatttccat atagcgcaac cagttaattg ctgaacccgc
      901 agtcatattg tctttcacca ccgcatctgc ggtatcttta tccagccgca aggtcattag
      961 cccgctattt gccagccaac catctttcac tatccatttt tcgttgttta gccataacgg
     1021 caatgcacca ctaaatgccc cggacatggc gaattgtttc ggattgacgg cgctaaccag
     1081 ttcgctggat gagagattat tcagccgcaa cagcgccggg tcatgttgcg gcatacgtaa
     1141 ttgtttcatc agcacgttac cgcctaacac atcgacgcta acatcagtca acagcaaggg
     1201 ttcttcttca gtccacggat aacgcccttg caaatcagcc gtaatatttt tcgctgtcac
     1261 cagattaatc acttcggcaa ttcgcaacgt aacggggccg cgagtcccca gatgccaggc
     1321 tccatcggca aaacggaaag gcaggacaaa atcgacaccg ttaacctgat tatctggcat
     1381 ccaggcacta ccgcctttca acacgccgtg ccctcccgcg cggaatcctt gttcaggcgc
     1441 agcagaaaat gcaacctgag catacagttc accatcgcgt aagttcatct tccagtcggg
     1501 tggcaccagc ggctggaata cagtcagtga ttgttttggc caccaggcgt taccgcgcag
     1561 acgaataccg tcccagcgac catttacccg aaccgggcca atctcaccag catgtaaatc
     1621 gcctttaaag aggaaatagg tcggattgcg cccatcgacg ctaaatttta aggttgatgg
     1681 cggtaacaca ctgccgccag tgaacagcgt ttgcccggcg tccagtgaca gcgcgccgct
     1741 gaaggaggga tgctgtgcgt cacgtaccca gacgacgggc ttgtcgagaa ttaatcgcgg
     1801 cttttctacc gtcatcgtac cgtattgtaa ctggtcgaaa ccggtggaga gatcggtcag
     1861 cgtaatcgtg ctgtcatgcc actcaccttt tcctgcgaca tcccaggtgg cattcatcgg
     1921 tgtaaaactc ccttttcccc agtagcgcca ctgccagcgg ccagcgtcag ggagaaaatc
     1981 attcgccagc ccatccatat gcagcacgaa atcgcccagt tcattttcat gcgcctgcaa
     2041 aatggcctgc aaacgtccgt caacaccacg ttgggtgact tttacacccg ctaaaggcca
     2101 gcggatttca tcgatgtcca gcgaatcgat gactcttccc tttgaacgaa gtaacgcgcc
     2161 tggctcaaag gtcagcgttg ggtcagacag acttccactt agctgcgcag gtaaacgggc
     2221 atataaaatg aggtccgcct gtttcgcttc accggtcagc tgcagaggca gttgactgtt
     2281 atccatgctt aattttcctg ggccaaaatt aagcaccgcg ttgcccttac ccgcttgccc
     2341 ctgggtcagc acattcagtc gtccgctgac cagagcgttc tcaagccctg cctgccagtt
     2401 gtcgactttg acacccagtc ggccactcaa aggaaaacca gcatacagcc agctccagcg
     2461 accatcgctt acggtcaatt gttgacgagt aatttgccac ggcaaatcga gcaacggatc
     2521 gccgttatcc cgtgccagca cgatcaattg cccgctattt tcctgccagt ccagctcggc
     2581 atccaccagt gacggttcct gcggcaagtt taacgtcgca gtagcatgcc cacttacagg
     2641 aagtccatcc ggcacgagcg gcatagtaaa ttcccccacc agtttaaccg gcggctgatt
     2701 ttcaaacgcg acgacatcca gttcgctgac tgtaagttgt tgccctttca gctggccttg
     2761 aaatttaact ttttcgccct gataacgcag ttgctggata tcagaggtta atgcgagaga
     2821 gagttttccc tgccattcct gccagggaga aaaaatcagt ttatcgatat tgatccaggt
     2881 gttaggcagc atggactgcc actgcgcgag ggtttttgga gcagctggcg attgctccgt
     2941 ctgcggcaat ttcgccagac aagcagaatc aagttctacc gtgccgacgt tcaataacca
     3001 gcggctggga tgtgaaaggc tggcgttggt gatatgcgca agctgacaat ctcccaccag
     3061 ataacggaga tcgggaatga ttaaaccttt acgcgtaatg cgtgggcttt catctaatgc
     3121 aatacgtgtc ccgagcggta gccagatgtc cgccagcgtg ggaacccaca gcccgagcgt
     3181 catcagcagc gtcaacggca caagaataat cagtaataac agcgcgagaa cggctttata
     3241 tttacccagc atgggtagtt aatatcctga tttagcgaaa aattaagcat tcaatacggg
     3301 tattgtggca tgtttaaccg ttcagttgaa ggttgcgcct acactaagca tagttgttga
     3361 tgaatttttc aatatcgcca tggctttcaa ttatatttga aattttgtaa aatattttta
     3421 gtagcttaaa tgtgattcaa catcactgga gaaagtctta tgaaactcgc cgtttatagc
     3481 acaaaacagt acgacaagaa gtacctgcaa caggtgaacg agtcctttgg ctttgagctg
     3541 gaattttttg actttctgct gacggaaaaa accgctaaaa ctgccaatgg ctgcgaagcg
     3601 gtatgtattt tcgtaaacga tgacggcagc cacccggtgc tggaagagct gaaaaagcac
     3661 ggcgttaaat atatcgccct gcgctgtgcc ggtttcaata acgtcgacct tgatgcggca
     3721 aaagaactgg ggctgaaagt agtccgtgtt ccagcctatg atccagaggc cgttgctgaa
     3781 cacgccatcg gtatgatgat gacgctgaac cgccgtattc accgcgcgta tcagcgtacc
     3841 cgtgacgcta acttctctct ggaaggtctg accggcttta ctatgtatgg caaaacggca
     3901 ggcgttatcg gtaccggtaa aatcggtgtg gcgatgctgc gcattctgaa aggttttggt
     3961 atgcgtctgc tggcgttcga tccgtatcca agtgcagcgg cgctggaact cggtgtggag
     4021 tatgtcgatc tgccaaccct gttctctgaa tcagacgtta tctctctgca ctgcccgctg
     4081 acaccggaaa actaccatct gttgaacgaa gccgccttcg atcagatgaa aaatggcgtg
     4141 atgatcgtca ataccagtcg cggtgcattg attgattctc aggcggcaat tgaagcgctg
     4201 aaaaatcaga aaattggttc gttgggtatg gacgtctatg agaacgaacg cgatctgttc
     4261 tttgaagata aatccaacga cgtgatccag gatgacgtat tccgtcgcct gtctgcctgc
     4321 cacaacgtgc tgtttaccgg gcaccaggca ttcctgacag cagaagctct gaccagtatt
     4381 tctcagacta cgctgcaaaa cttaagcaat ctggaaaaag gtgaaacctg cccgaacgaa
     4441 ctggtttaat cttgcagctc ccctgcaatc caggggagct gattcagata atccccaatg
     4501 acctttcatt ctctattctt aaaatagtcc tgagtcagaa actgtaattg agaaccacaa
     4561 tgaagaaagt agccgcgttt gttgcgctaa gcctgctgat ggcgggatgt gtaagtaatg
     4621 acaaaattgc tgttacgcca gaacagctac agcatcatcg ctttgtgctg gaaagcgtaa
     4681 acggtaagcc cgtgaccagc gataaaaatc cgccagaaat cagctttggt gaaaaaatga
     4741 tgatttccgg cagcatgtgt aaccgcttta gcggtgaagg caaactgtct aatggtgaac
     4801 tgacagccaa agggctggca atgacccgta tgatgtgcgc taacccgcag cttaatgaac
     4861 tcgataac
//
LOCUS       DAJTRC010000186         4728 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.188, whole genome shotgun sequence.
ACCESSION   DAJTRC010000186 DAJTRC010000000
VERSION     DAJTRC010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4728)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4728)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4728
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.188"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(78..866)
                     /gene="phoH"
                     /locus_tag="OO357_003950"
     CDS             complement(78..866)
                     /gene="phoH"
                     /locus_tag="OO357_003950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate starvation-inducible protein PhoH"
                     /protein_id="HCR5888704.1"
                     /translation="MGRQKAVIKARREAKRVLRRDSRSHKQREEESVTSLVQMSGVEA
                     IGMARDSRDTSPILARNEAQLHYLQAIESKQLIFATGEAGCGKTWISAAKAAEALIHK
                     DVDRIIVTRPVLQADEDLGFLPGDIAEKFAPYFRPVYDLLVRRLGASFMQYCLRPEIG
                     KVETAPFAYMRGRTFENAVVILDEAQNVTAAQMKMFLTRLGENVTVIVNGDITQCDLP
                     RGVCSGLSDALERFEEDEMVGIVRFGKEDCVRSALCQRTLHAYS"
     gene            complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="OO357_003951"
     CDS             complement(1473..2738)
                     /gene="efeB"
                     /locus_tag="OO357_003951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deferrochelatase/peroxidase EfeB"
                     /protein_id="HCR5888705.1"
                     /translation="MQYKDENGVNEPSRRRLLKGIGALAGSCPVAHAQKTQSAPGTLS
                     PDARNEKQPFYGEHQAGILTPQQAAMMLVAFDVLASDKADLERLFRLLTQRFAFLSQG
                     GAAPETPNPRLPPLDSGILGGYIAPDNLTITLSVGHSLFDERFGLAPQMPKKLQKMTR
                     FPNDSLDAALCHGDVLLQICANTQDTVNHALRDIIKHTPDLLSVRWKREGFISDHAAR
                     SKGKETPINLLGFKDGTANPDSQNDKLMQKVVWVTADQQEPAWTIGGSYQAVRLIQFR
                     VEFWDRTPLKEQQTIFGRDKQTGAPLGMQHEHDVPDYASDPEGKVIALDSHIRLANPR
                     TAESESSLMLRRGYSYSLGVTNSGQLDMGLLFVCYQHDLEKGFLTVQKRLNGEALEEY
                     VKPIGGGYFFSLPGVKDANDYLGRALLQV"
     gene            complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="OO357_003952"
     CDS             complement(2744..3871)
                     /gene="efeO"
                     /locus_tag="OO357_003952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron uptake system protein EfeO"
                     /protein_id="HCR5888706.1"
                     /translation="MTINFRRNALQLSVAALFSSAFMANAADVPQVKVTVTDKQCEPM
                     TITVNAGKTQFIIQNHSQKALEWEILKGVMVVEERENIAPGFSQKMTANLQPGEYDMT
                     CGLLTNPKGKLIVKGEATADAAQSDALLSLGGAITAYKAYVMAETTQLVTDTKAFTDA
                     IKAGDIEKAKALYAPTRQHYERIEPIAELFSDLDGSIDAREDDYEQKAADPKFTGFHR
                     LEKALFGDNTTKGMDQYAEQLYTDVVDLQKRISELAFPPSKVVGGAAGLIEEVAASKI
                     SGEEDRYSHTDLWDFQANVEGSQKIVDLLRPQLQKANPELLAKVDANFKKVDTILAKY
                     RTKDGFETYDKLTDADRNALKGPITALAEDLAQLRGVLGLD"
     gene            complement(3929..4603)
                     /locus_tag="OO357_003953"
                     /pseudo
     CDS             complement(3929..4603)
                     /locus_tag="OO357_003953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309290.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
BASE COUNT         1112 a         1328 c         1140 g         1148 t
ORIGIN      
        1 ttcagaaaaa gcgctagtcc gggcataaac acccaaaaca aagcccggtt cgcccgggct
       61 ctgcaccgat aatacactta actgtatgca tgcagcgtac gttggcaaag tgccgaacgt
      121 acgcaatcct cttttccgaa ccggacgatc ccaaccattt catcttcttc gaaacgttcc
      181 agcgcgtcac ttaatccgga gcacacgccg cgaggcaaat cgcattgcgt gatatcaccg
      241 ttgacgataa ccgtcacgtt ctccccgagg cgggttaaaa acattttcat ttgcgcggca
      301 gtcacattct gcgcctcgtc aagaatgaca actgcatttt caaaggtacg tccacgcata
      361 taggcgaacg gcgcggtttc caccttccct atttccggtc gcaggcagta ctgcataaag
      421 gaagccccta agcgccggac cagcaagtcg tagaccgggc gaaaataggg agcaaacttt
      481 tctgcgatat caccaggtaa gaagccaaga tcttcatcgg cttgcagaac tggacgggtg
      541 acgataatcc tgtcgacatc cttatgtatc agggcctctg ccgcttttgc tgcgctgatc
      601 caggtttttc cgcacccggc ttcgcccgtg gcgaatatca gctgcttact ctcaatagcc
      661 tgcagatagt gcaattgcgc ttcatttcgc gcaaggatgg gcgaggtatc gcgactgtcg
      721 cgggccatac caatggcttc tacgccgctc atctgcacaa gcgaggtgac cgattcttct
      781 tcacgctgct tatggctgcg cgaatcccgt ctcagcacac gttttgcctc gcgacgagct
      841 ttgatcactg ctttttgtct tcccatggag agcaccttga gttgtttgta ttcatcacac
      901 gcgccgttgg cagcgcgatt atgcgcacga acatcagagg gttggcttcc ttgtaagcca
      961 tagtttgctt ttggataaaa tgccgaaaaa cggctacgca caccgtttgc ggcgtcggta
     1021 acacgagtaa agaaaggaag tgagaaaaat acagagtgac gaagttgcta ccggaggaga
     1081 aatctccgcg agtggtggag cgttgcttaa caaaacattg tccagaacag gacgttacca
     1141 tccgcgatct ccatagtgac tgactatcac tgccgggaac ttccgctgct acttaataag
     1201 tacaacagaa ctcgcattca ttgcaacaat atattcactt atatttaacg ataacaccca
     1261 tttcggtgac attaatttct actggaaaga tgacagagtg atgacagtga tggaaaaagc
     1321 tgtgtgcttt ccgcaggatt cgcagggctg gtaaaatgtg cctcgtgttt gccggattcg
     1381 gcgcgaacgc cttacccgac ctacaagtga ttaataattt gtgcacaaaa ctcatgcccg
     1441 ataaacctgc gcatcgggca tatttgcgtc gttcaaacct gcaatagcgc ccttcccaga
     1501 taatcgttcg cgtccttcac tcccggcagc gaaaaaaaat aaccgccgcc gataggttta
     1561 acgtattcct ccagcgcttc gccattgagc cttttttgta ctgtcaggaa gcctttttcc
     1621 agatcgtgtt ggtagcagac aaacagcaac cccatatcca gttgtccgga gttggtgacc
     1681 cccaacgaat aactgtagcc gcgacgcagc atcaggctgg actcactctc cgccgtgcgg
     1741 ggattcgcca gccggatatg gctgtccagc gcgatcacct tcccttccgg gtcgctggcg
     1801 taatcaggca catcatgctc atgctgcatt cccagcggcg caccggtttg cttatcacgg
     1861 ccaaaaatcg tctgctgttc tttcagcggc gttctgtccc aaaattccac tcgaaactga
     1921 atcaagcgta ctgcctgata gctgccaccg attgtccacg caggctcctg ctgatctgcc
     1981 gttacccaca ccactttttg catcaactta tcattctggc tatcgggatt ggcagtgccg
     2041 tctttgaaac ccagcaaatt aatcggcgtc tctttgcctt tactacgcgc cgcgtgatcg
     2101 gaaataaacc cttcccgctt ccagcgcaca ctgagcaaat ccggcgtgtg tttgatgata
     2161 tcgcgcagcg catggttcac cgtgtcctgg gtgttggcgc aaatctgtag caacacatca
     2221 ccatgacata acgccgcatc cagcgagtcg ttggggaaac gcgtcatctt ctgcagcttt
     2281 tttggcatct gtggcgcaag gccaaagcgc tcatcaaaca atgagtgacc caccgataac
     2341 gtgatggtga gattatcggg cgcaatgtag ccgccaagaa tgccggaatc gagtggtggc
     2401 aggcgcggat ttggcgtttc tggtgctgct ccgccctgac tcagaaaagc aaaacgctga
     2461 gtcaacaagc gaaacaaccg ctcaagatcg gctttatcgc tggcaagcac atcaaacgcc
     2521 accagcatca ttgcggcctg ttgtggcgtc aggatccctg cctgatgctc accataaaac
     2581 ggctgtttct cattgcgtgc atccggtgaa agcgtgcccg gtgcactttg cgttttttgt
     2641 gcatgagcga ccggacaact tcccgccagc gcaccaatcc ctttcagtaa acgtcggcgt
     2701 gacggttcat tcacgccgtt ttcatcttta tactgcataa cgcttaatcc agtcccagca
     2761 caccgcgaag ttgcgccaga tcttccgcca gcgcagtaat cggccctttc agcgcattcc
     2821 ggtcggcatc ggtcaatttg tcgtaggttt caaaaccgtc tttagtacgg tatttcgcca
     2881 gaatggtatc gaccttttta aagttggcat cgacttttgc caacagttcc gggttggctt
     2941 tttgcagttg tggacgcagc agatcgacaa ttttctgcga gccttcaacg ttagcctgga
     3001 aatcccacag atcggtgtgg ctgtagcgat cttcttcacc gctgattttg ctggctgcca
     3061 cttcctcaat cagtccggct gcgccgccga ccacttttga aggtgggaaa gccagttcac
     3121 tgatgcgttt ttgcaaatcg accacatcgg tataaagctg ctcagcgtac tgatccatcc
     3181 ctttggtggt gttgtcgcca aacaatgctt tttccagacg gtggaaacca gtgaattttg
     3241 ggtcggcggc tttttgctcg taatcatctt cacgggcgtc aatgctgcca tccagatcgg
     3301 agaacagttc agcaatcggt tcaatacgct catagtgctg gcgcgtcggc gcatacagtg
     3361 ctttcgcttt ttcgatatcg cctgctttaa tcgcgtcggt aaaggctttg gtgtcggtca
     3421 ccagctgcgt ggtttccgcc atgacatacg ctttatatgc agtaattgca ccaccaagac
     3481 ttaacagcgc atcactttgc gccgcatccg ccgttgcctc acctttgacg atcaacttcc
     3541 ctttcgggtt agtcagcaga ccgcaggtca tatcgtattc gccaggctgt aaattcgccg
     3601 tcattttctg gctaaaacca ggggcgatat tttcccgctc ttccaccacc atcacgcctt
     3661 tgaggatctc ccactccagc gccttctggc tatggttctg aataatgaac tgtgttttcc
     3721 cggcgttaac cgtaatggtc atcggttcgc actgcttatc cgtcacggtc actttgacct
     3781 gcggcacatc agcggcgtta gccataaaag cagaagaaaa cagcgcagcc acgctcaact
     3841 gcaatgcgtt acggcggaag ttaatggtca tgacgagact atccctttaa agtatgttgt
     3901 aactaagcaa gagtttgcgt cgtatttgtt acgcggagcg agacgctgtc gcccctgcgc
     3961 gtggtggcag agcaaatgcc accagcgccg ggatgagata aataaaccag acggcgactt
     4021 cgctgacgct cggtgcttcc tgatagccaa aaatcccttc catcagcgtg ccaaacagcg
     4081 agtgagttga gaggaccgcg ctcatatcga aggcgatttc ctgaaagtgg ttccacaatc
     4141 cggcttcatg aaatgcgcga atggcaccag ccgccagccc tgcggcgacg aagagaataa
     4201 acaggctggt ccatttaaaa aatgcaccaa gattgaggcg aataccgccc cagtagagca
     4261 ggaagccgag caccacggca gtagcaagac cgagcattgc acccagcggc ggccagatcc
     4321 cgacatcttg ttgaaatgcc gccagcagga aaaagaccga ctccagccct tcccttgcaa
     4381 cggcaaaaaa gaccatcatc accagcgccc agccatgatg atttccacgc tgcaatgcgc
     4441 tatcgactgc ctgttccagt tgcactttga cgttgcgcga cactttgcgc atccagaaaa
     4501 ccatccaggt aaggatcacc acggcgatca ccgccacgat accttcaaac agttcctgct
     4561 ctttttgcgg aaattcgccg gtggtttcgt taatgaagat ccccgagtag gcagcctgac
     4621 ggctgcggct tgtcatggtc tgcaattacc gttataaaaa caggcaatgt cattgtcttt
     4681 caggtggtta tatggcccgt tcagctaaac cccgtaaacg taaacccg
//
LOCUS       DAJTRC010000187         4638 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.189, whole genome shotgun sequence.
ACCESSION   DAJTRC010000187 DAJTRC010000000
VERSION     DAJTRC010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4638)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4638)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4638
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.189"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(1..494)
                     /locus_tag="OO357_003954"
                     /pseudo
     CDS             complement(1..494)
                     /locus_tag="OO357_003954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3-like element IS3H family transposase"
     gene            637..759
                     /locus_tag="OO357_003955"
                     /pseudo
     CDS             637..759
                     /locus_tag="OO357_003955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1021..1587)
                     /gene="ipgB2"
                     /locus_tag="OO357_003956"
     CDS             complement(1021..1587)
                     /gene="ipgB2"
                     /locus_tag="OO357_003956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858157.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion effector IpgB2"
                     /protein_id="HCR5888707.1"
                     /translation="MLGTSFNNFGISLSHKRYFSGKVDEIIRCTMGKRIVKISSTKIN
                     TSILSSVSEQIGENITDWKNDEKKVYVSRVVNQCIDKFCAEHSRKIGDNLRKQIFKQV
                     EKDYRISLDINAAQSSINHLVSGSSYFKKKMDELCEGMNRSVKNDTTSNVANLISDQF
                     FEKNVQYIDLKKLRGNMSDYITNLESPF"
     gene            complement(1869..2183)
                     /locus_tag="OO357_003957"
     CDS             complement(1869..2183)
                     /locus_tag="OO357_003957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888708.1"
                     /translation="MLQRQRGKVGFAQLPVDFVAIEPDSVQGVGKRANLTNRCFIIRI
                     NDSFKKRQGFIEFISNSGSGHTVTVYTKRRFQRGVFMNSLNTNVVKPVMYRLRSSADA
                     RP"
     gene            complement(2261..2371)
                     /locus_tag="OO357_003958"
                     /pseudo
     CDS             complement(2261..2371)
                     /locus_tag="OO357_003958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2695..3372)
                     /gene="ospD1"
                     /locus_tag="OO357_003959"
     CDS             complement(2695..3372)
                     /gene="ospD1"
                     /locus_tag="OO357_003959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector/anti-activator
                     OspD1"
                     /protein_id="HCR5888709.1"
                     /translation="MSINNYGLHPANNKNMHLIIGSNTANENKGMKNNIINVTNTAIS
                     HAINEEKSGGGYSGVSFRKLAKIQNISIPTKNNKEYNRHNLFSLIWHGNADAARKYGE
                     SLLAAEIPKEEKLEVLAARNNAGESALFIALQEGHSAAIQAYGDFIKTFDLSPKETIK
                     LLDVRDNEGLPGLFLAAGKGNIEAMMAYINICHHSGIKLTEIADRLNNNEQDMFNIIS
                     DKIQELF"
     gene            complement(3744..3881)
                     /locus_tag="OO357_003960"
                     /pseudo
     CDS             complement(3744..3881)
                     /locus_tag="OO357_003960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
     gene            complement(3963..4261)
                     /locus_tag="OO357_003961"
                     /pseudo
     CDS             complement(3963..4261)
                     /locus_tag="OO357_003961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343747.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT         1262 a          994 c          900 g         1482 t
ORIGIN      
        1 cgccagcgat tgtaatcaca ttcgatataa ttaaacactg ttgccctcat tatttcccgg
       61 ctgataaagt gttctccgtg gagacattcc actttcagcg aatgaaagaa gctttccacg
      121 caggcattat cgtagcagca gccctttgcg ctcatactgc cacgcagatt atgccacttc
      181 agcagcgcct gataatctgc tgaacagtac tgacttcctc ggtccgagtg aacgataacg
      241 ttccggggtc tcttacgccg ccacagcgcc atctgcaggg catcgcaggc cagttgcgcc
      301 gtcaggcgtg gtgacatgga ccagccaata acggcacgtg accacaggtc aatgaccaca
      361 gccagataca gccagccttc atctgtacgt aagtacgtga tgtctcccgc ccacttctgg
      421 ttcggaccac tggcgtaaaa atcctgctcc agcagatttt ctgacacagg caggccatgt
      481 gcgcggtaac tgaccgtatt gctgggtaag atcaatttgc tcactggtat ttactacgtg
      541 catcgaatga gtgatcaaga gccgtatgac gcgagagtgt cacgtacggt tccgtaagca
      601 gccggagagt gaaactctct ccggctgact cgactcgcag gttccgacag cggtgacgaa
      661 catgcagcgg tgttgttctc gctgatcggc acatgccgtc tgaacaatgt ggagccagaa
      721 aaatggctgc gttacgttat tgaacatatc caggactgac cggcaaaccg ggtacgcgat
      781 ctgttgcctt ggaaagttga tctgacttct cagtaaatat caatatgatt ctgacgagcc
      841 gcttaccttt gagcagagaa aggggagagg gatcccacat ccatgagcta ggaaaataca
      901 aaaactggct atacaacaat tcaatatatt aggacttact tgcgaggaga aatatagcac
      961 cgctcaggca attcctgggt atttactttt cttagtttct ttactcggga tgttctgtca
     1021 tcagaaaggc gattctaaat ttgtaatata gtcactcata ttcccacgta atttctttag
     1081 atcaatatat tgcacatttt tctcaaaaaa ctgatcggaa ataagatttg caacattgct
     1141 tgttgtatca tttttaacgc ttctattcat cccttcacaa agttcgtcca ttttcttttt
     1201 gaaataactg cttcctgata caagatgatt tatggaactt tgtgcagcat tgatgtctag
     1261 agaaatgcga taatctttct caacctgttt aaatatctgt ttacggagat tatcgccgat
     1321 ttttcggctg tgttctgcac aaaacttatc aatacattgg tttaccaccc gggatacgta
     1381 gacttttttt tcatcatttt tccaatctgt aatattttca ccgatttgtt ctgaaacaga
     1441 gctcaaaata ctagtattta ttttggttga tgatattttt actatccttt tccccattgt
     1501 acagcggata atctcatcca cctttcctga aaaatatctt ttgtgagata aactgattcc
     1561 aaaattatta aaagatgttc caagcattat aatcaccttg tcatgtaaaa tagtaacatt
     1621 gtcattattt tagctgattc aaacaattct gtaaatgctt tgagttcttt actcccttgg
     1681 ctcaatgatg gaccgtctgg aatcccagag actaaactct ctgagaaaga gagtatgaaa
     1741 aaacttgggg cagtccctaa atgaagggac caacagcggt aaaataaccg gcatcattca
     1801 taatgatgcc ctgttagttg tagttatgaa gatttacgtc attaaaattg ctgttcgtag
     1861 tgtcagtcct atggtctggc atcagctgag gatcgtagcc gatacatcac tggcttcacg
     1921 acattcgtgt tgaggctatt catgaaaact ccacgctgaa aacgccgttt tgtataaacg
     1981 gtcacggtat gcccggagcc actgttgctg ataaattcga taaaaccttg gcgtttcttg
     2041 aagctatcgt taatgcggat aatgaaacaa cgattggtga gattcgctct tttgccgacg
     2101 ccctggacgc tgtccggttc aatcgcaaca aaatcaaccg gcagctgagc aaacccaacc
     2161 ttgcctctct ggcgctggag catgaagtga tttggctggg acgtagccgc tgacagcttc
     2221 tataaaagta tgatcctacc cacgtaatat gaacacaggc ttaagtgagg ttctggtttt
     2281 caaattgttc aggactgaga cctccacacc aactgtgccg tcgtcagcga ttgtaatcgc
     2341 acttgatata attaaacact gtatatttca tttgtggagt ctccagtctg agagggcttt
     2401 atgcattccg tattgggcac tatgatgcca gaaatctgca aggctccaca ccttcctgta
     2461 tttcccttcc tctatcacag tgcactttac ttaatagggt gggatgtgtt cattacattt
     2521 attgtgtttt ttatgattgt ttttgcgtcc agtgtcgttc gtcatgggaa actggttcta
     2581 tgggtagtat cgctcagtct agttaactgt ttgtttcttt ttagaaatta agcagatcac
     2641 acaattcggg gagagatcct tctaagtgca attctttgca gctatttagc acacttaaaa
     2701 caactcttgt attttgtcag aaataatatt aaacatgtct tgttcattat tgttaagtct
     2761 gtctgctatt tctgtaagtt ttatcccact atgatggcat atatttatat atgccatcat
     2821 agcctcgata ttccctttcc ctgcggccag aaataatcct ggtaacccct cattatctct
     2881 tacatccaat agtttaatcg tttcttttgg tgataaatca aaagttttaa taaaatctcc
     2941 ataagcttga atcgcagcgg aatgaccttc ttgaagagct atgaacaaag cagattcccc
     3001 agcattattt cgtgctgcaa gaacttctag tttttcctct ttgggtattt cggctgccaa
     3061 cagcgattca ccgtatttac gcgctgcatc ggcatttcca tgccaaatca atgaaaacaa
     3121 attatggcgg ttatactcct tattattctt tgtcggaatg gatatgttct gtattttggc
     3181 caattttctg aaagaaacac cactatatcc cccccctgat ttttcttcat tgatggcgtg
     3241 ggatatagcg gtatttgtca cgttaatgat attatttttc attcctttat tttcattagc
     3301 agtattgctg cctattatta agtgcatatt tttgttgttt gctggatgta atccatagtt
     3361 atttattgac atttttgttc atcctttttg ctaaaaaaaa acaataacct atttttgtaa
     3421 tgtaatagat attagtctat tgctaatcta aaaaaaccat tgccacattg aatggctata
     3481 ttcaccaata aattctttcg atttcgtata ttgacatatc gtagatatat cttatcccta
     3541 agattcaata atcgagtccg tttaccattc tgtgcaagcg ccttcactca gaagtgaccg
     3601 tcaaggcgat caccaaactt gataataaag cagctcattg ccatacgcca gtccttaagg
     3661 ggcgttgtcc atttctgtga tgctgcctag atttaactgc cagctccggc ttatctacat
     3721 ctaaatatgt gagcctaata ccgctacact ttgtccagac tatgtttttc tccgggaagt
     3781 gggctaaccg tctccataaa gtgacgaact tttttcaaca gtggccatat tgaacggcac
     3841 tgatgattac gggttattgt ctcatgaaga gccggccaca tcactgacag cagtgacttc
     3901 aggccttcag caccagacag cctgaaccag ttaatccatc gcccgacaaa tttagtaatc
     3961 tagggctggt ttttcttcag ctgttgcggt tcgaaagtgc cattacgatc acgcagagta
     4021 cggatttcca gaggttcatc tccagtagtg ataatcttcg ttaagcaacc attgcgggaa
     4081 ttgctgccag atctaagctg atttttctcg cacccaagat gatgagtcat attagcattg
     4141 agtacggttt cattttcagc atccaatcaa actgactgag atcttcagga tttttgagac
     4201 ttttggctag ttcattagct aaagcccgca acttattaga tgtcaactgt ttttcattca
     4261 taaattcacc ttcatttgat atcaaattaa atagttcaac atcagagaat tatacaaact
     4321 tatgtacagg ctcctatgat cttcgaatcc tgaagattat agtgaaaata agttttcacg
     4381 ttgaaatccc acatagttag tatatgataa ataatcatgg atgacctgat taacctcttg
     4441 gggtagtgct tttatagaag aataaatatt ctactaattt aatacatcac ctaaaatagt
     4501 attgtttttt atggttggtt gaaggtcgtg gtgatgatgt tagtcctttt gagaatattc
     4561 cagtgccaga tcttacttat aataaagggg cttggcagtt tggataaact attgttctta
     4621 ttccactaat acaaactt
//
LOCUS       DAJTRC010000188         4630 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.190, whole genome shotgun sequence.
ACCESSION   DAJTRC010000188 DAJTRC010000000
VERSION     DAJTRC010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.190"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            119..4237
                     /gene="pic"
                     /locus_tag="OO357_003962"
     CDS             119..4237
                     /gene="pic"
                     /locus_tag="OO357_003962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708747.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Pic"
                     /protein_id="HCR5888710.1"
                     /translation="MNKVYSLKYCPVTGGLIAVSELARRVIKKTCRRLTHILLAGIPA
                     ICLCYSQISQAGIVRSDIAYQIYRDFAENKGLFVPGANDIPVYDKDGKLVGRLGKAPM
                     ADFSSVSSNGVATLVSPQYIVSVKHNGGYRSVSFGNGKNTYSLVDRNNHPSIDFHAPR
                     LNKLVTEVIPSAVTSEGTKANAYKYTERYTAFYRVGSGTQYTKDKDGNLVKVAGGYAF
                     KTGGTTGVPLISDATIVSNPGQTYNPVNGPLPDYGAPGDSGSPLFAYDKQQKKWVIVA
                     VLRAYAGINGATNWWNVIPTDYLNQVMQDDFDAPVDFVSGLGPLNWTYDKTSGTGTLS
                     QGSKNWTMHGQKDNDLNAGKNLVFSGQNGAIILKDSVTQGAGYLEFKDSYTVSAESGK
                     TWTGAGIITDKGTNVTWKVNGVAGDNLHKLGEGTLTINGTGVNPGGLKTGDGIVVLNQ
                     QADTAGNIQAFSSVNLASGRPTVVLGDARQVNPDNISWGYRGGKLDLNGNAVTFTRLQ
                     AADYGAVITNNAQQKSQLLLDLKAQDTNVSEPTIGNISPFGGTGTPGNLYSMILNSQT
                     RFYILKSASYGNTLWGNSLNDPAQWEFVGMNKNKAVQTVKDRILAGRAKQPVIFHGQL
                     TGNMDVAIPQVPGGRKVIFDGSVNLPEGTLSQDSGTLIFQGHPVIHASISGSAPVSLN
                     QKDWENRQFTMKTLSLKDADFHLSRNASLNSDIKSDNSHITLGSDRAFVDKNDGTGNY
                     VIPEEGTSVPDTVNDRSQYEGNITLNHNSALDIGSRFTGGIDAYDSAVSITSPDVLLT
                     APGAFAGSSLTVHDGGHLTALNGLFSDGHIQAGKNGKITLSGTPVKDTANQYAPAVYL
                     TDGYDLTGDNAALEITRGAHASGDIHASAASTVTIGSDTPAELASAETAASAFAGSLL
                     EGYNAAFNGAITGGRADVSMHNALWTLGGDSAIHSLTVRNSRISSEGDRTFRTLTVNK
                     LDATGSDFVLRTDLKNADKINVTEKATGSDNSLNVSFMNNPAQGQALNIPLVTAPAGT
                     SAEMFKAGTRVTGFSRVTPTLHVDTSGGNTKWILDGFKAEADKAAAAKADSFMNAGYK
                     NFMTEVNNLNKRMGDLRDTNGDAGAWARIMSGAGSADGGYSDNYTHVQVGFDKKHELD
                     GVDLFTGVTMTYTDSSADSHAFSGKTKSVGGGLYASALFESGAYIDLIGKYIHHDNDY
                     TGNFASLGTKHYNTHSWYAGAETGYRYHLTEDTFIEPQAELVYGAVSGKTFRWKDGDM
                     DLSMKNRDFSPLVGRTGVELGKTFSGKDWSVTARAGTSWQFDLLNNGDTVLRDASGEK
                     RIKGEKDSRMLFNVGMNAQIKDNMRFGLEFEKSAFGKYNVDNAVNANFRYMF"
     gene            complement(4388..>4630)
                     /locus_tag="OO357_003963"
     CDS             complement(4388..>4630)
                     /locus_tag="OO357_003963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_025243830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HCR5888711.1"
                     /translation="AYPTDIRKVIYTTNAIESLNSVIRHAIKKRKVFPTDDSVKKVVW
                     LAIQSASQKWTMPLKDWRMAMSRFIIEFGDRLDGHF"
BASE COUNT         1240 a         1050 c         1225 g         1115 t
ORIGIN      
        1 acaaaaacac attaatgcag taactatatt ttcctttctg gtgataacgt cgggttatca
       61 ttagcttctt cagctatttt acttttatat cccttgtaaa catcatggag aatccatagt
      121 gaataaagtt tattctctta aatattgccc cgtcaccggg gggcttattg ctgtctctga
      181 acttgcccgc agggtaataa aaaagacatg ccgaagatta acgcatattc ttctggctgg
      241 cattccagca atctgtctgt gttactctca gatatcccag gcgggtattg tccgttccga
      301 tattgcctat cagatttatc gtgatttcgc cgaaaacaaa gggctttttg tacctggtgc
      361 caatgatatt ccggtatatg ataaggacgg aaaacttgtg ggaagactgg gtaaagcccc
      421 aatggccgat ttcagcagtg tgagctcaaa tggcgttgct acgcttgtat cgcctcagta
      481 tatcgtcagc gtaaagcata acggaggata tcggagtgtg agctttggta atgggaaaaa
      541 tacatattcc cttgttgacc gtaataacca cccttctatt gacttccatg ctccacgtct
      601 gaataaactg gttacagaag ttattccctc agcggtaaca tcagaaggaa ccaaagccaa
      661 tgcttataaa tacactgaac gttacaccgc tttttatcgg gtgggtagtg gtacgcagta
      721 cactaaggac aaggacggaa atttagttaa ggttgccggt ggatatgctt ttaaaacagg
      781 aggaaccaca ggagttcctc tgatatctga tgcaacaata gtctctaatc ccgggcaaac
      841 ttataatcct gtaaacggcc ctttacctga ctatggagcc cctggggata gtggttctcc
      901 tttgtttgct tatgataaac aacaaaaaaa atgggttatt gttgctgtat taagagcata
      961 tgcaggtatt aatggtgcta cgaactggtg gaatgtcata ccaacagatt atctgaacca
     1021 ggttatgcag gacgatttcg atgcccccgt agactttgtt tccggactgg gccccctgaa
     1081 ctggacatac gacaaaacat caggcacagg taccctgagc cagggcagta aaaactggac
     1141 catgcacggg cagaaagaca atgacctcaa tgccggtaaa aatctggtat tcagcgggca
     1201 gaatggtgca attatcctga aagacagtgt gactcagggt gccggttatc tcgaatttaa
     1261 agacagttac accgtatctg ctgaatccgg aaaaacatgg acgggtgccg gcattattac
     1321 tgacaagggg acgaatgtaa cctggaaggt caacggcgtt gccggtgaca acttgcataa
     1381 gctgggggaa ggaaccctga ccataaacgg aacaggtgta aacccgggag gactgaaaac
     1441 gggagacggt atcgttgtac ttaaccagca ggcagacact gcaggtaata tccaggcctt
     1501 cagttcagtg aacctcgcca gcggacgtcc gaccgtggtg ctcggggatg cccgtcaggt
     1561 caatccggat aacatttcat ggggataccg gggaggtaag cttgacctta atggtaatgc
     1621 cgttaccttc acccgactgc aggctgctga ttacggggcg gtgattacaa ataatgcaca
     1681 gcaaaaatcc cagcttttac tggatcttaa ggctcaggat acaaatgtca gtgaaccgac
     1741 gattggaaat atatccccct ttggtggtac cggcactcca ggaaacctgt acagcatgat
     1801 actcaacagc cagacccgct tctatattct gaaatctgcc agctatggta acactctgtg
     1861 ggggaacagc ctgaatgatc cggctcagtg ggagtttgtt ggcatgaaca aaaacaaagc
     1921 agttcagaca gtaaaagata ggatcctggc cgggcgggca aaacaacccg ttatctttca
     1981 tggtcagctg accgggaata tggatgtcgc cattccacag gtgccggggg gaagaaaggt
     2041 aatctttgat ggtagcgtga acctgccgga aggtaccctg agtcaggaca gtggcaccct
     2101 gatattccag ggacatccgg ttatccatgc ctccatcagt ggcagtgcac cggtcagcct
     2161 gaaccagaaa gactgggaaa accgtcagtt tacaatgaaa acactgtcgc tgaaagacgc
     2221 tgacttccat ctttcacgta acgcctcgct gaacagtgac attaagtcgg ataacagcca
     2281 tatcacactg ggaagtgaca gggcatttgt ggataaaaat gacggaacag gaaattatgt
     2341 cattccggag gaaggtacct ctgtcccgga caccgtgaat gacaggagcc agtatgaagg
     2401 gaatattacg ctgaaccata actcagccct ggatatcggc agcaggttca ccggggggat
     2461 tgacgcttat gacagtgccg tcagcatcac ctctccggac gtcctgttga cagccccggg
     2521 tgcttttgcc ggcagttcac tgacagtgca tgatggcggt catcttacag cactgaacgg
     2581 tcttttcagc gacgggcata ttcaggccgg taagaacggc aaaatcaccc tgagcggtac
     2641 accggttaaa gatacggcta atcagtatgc ccctgctgta tatctgacgg acggatatga
     2701 cctgaccggc gataacgcag cactggaaat tacccgtgga gcacatgctt ccggtgatat
     2761 tcatgcctct gcggcatcaa cagttaccat cgggtctgac acgccggcag aactggcttc
     2821 tgcggaaacg gctgcatcgg cgtttgccgg cagtcttctt gagggctata acgcagcatt
     2881 caatggtgcc ataaccggtg gcagggctga tgtcagtatg cataatgcac tgtggactct
     2941 gggtggggac tctgccatcc acagtcttac cgtcagaaac agccgtatta gttctgaagg
     3001 agaccgtaca ttccgtaccc tgacggtgaa taaactggat gcaacaggca gtgattttgt
     3061 tttgcgtacg gacctgaaaa atgccgataa aattaatgtg actgaaaaag ccactggttc
     3121 agataacagc ctgaacgtca gctttatgaa taatcctgct cagggacagg ccctgaatat
     3181 tcctctggtc acggcaccgg cgggaacttc agcagagatg tttaaggccg gcacccgggt
     3241 gacaggtttc agtcgggtga ccccaaccct gcatgttgat accagtggtg gcaatacgaa
     3301 gtggatactg gatggtttta aagcggaggc tgataaagcc gctgccgcga aggctgacag
     3361 tttcatgaat gccgggtata aaaacttcat gacggaagtt aacaatctga acaaacgtat
     3421 gggtgacctg cgtgacacaa acggtgatgc cggtgcctgg gcgcgcatca tgagtggtgc
     3481 cggttctgca gacggtggtt acagtgataa ttacacccat gttcaggtcg gctttgacaa
     3541 aaaacatgaa ctggacggtg tggacctgtt taccggtgtc acgatgacct ataccgacag
     3601 cagtgcagac agccatgcat tcagcggaaa gacgaaatcg gtggggggcg gtctgtatgc
     3661 ttcagcattg tttgagtccg gtgcctatat cgatttgatt ggtaaatata ttcaccatga
     3721 caatgattac acaggtaact ttgctagcct gggaacgaaa cactacaaca cccattcctg
     3781 gtatgccggt gctgaaacgg gttaccgcta tcacctgaca gaggacacgt tcattgagcc
     3841 gcaggctgaa ctggtttacg gcgccgtgtc cgggaaaaca ttccgctgga aagacggtga
     3901 tatggacctg agcatgaaga acagggactt cagtccgctg gttggaagaa caggggttga
     3961 actgggcaag accttcagtg gtaaggactg gagtgtgacg gcccgtgccg gaaccagctg
     4021 gcagtttgac ctgctgaata atggagatac cgtactgcgt gatgcgtccg gggagaaacg
     4081 gataaaagga gagaaggaca gccggatgct gtttaatgtt ggtatgaatg cgcagataaa
     4141 ggacaatatg cgctttggtc tggagtttga gaagtcagcc tttggtaaat ataacgtgga
     4201 taatgcggta aacgcgaatt tccggtatat gttctgatgt gggggtaaag ggctgccgga
     4261 aggcagcctt tctcagagac tcttaaaata taaaactgaa ggtcttcggt aagggggagt
     4321 gtattagacg tcaactagtt ttgccgactg gcgcgagcct gtttaagatt ctgtgtaaat
     4381 gcctttctca gaagtgaccg tccaggcggt caccgaactc gataataaag cggctcattg
     4441 ccattcgcca gtccttcaac ggcatcgtcc atttctggga cgcagactgg attgccagcc
     4501 acaccacttt tttcaccgag tcgtctgtcg ggaacacttt acgctttttg atcgcatggc
     4561 ggatcacgct gtttagcgac tcgatggcat tcgtcgtata gatcactttg cggatgtccg
     4621 ttggataagc
//
LOCUS       DAJTRC010000189         4430 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.191, whole genome shotgun sequence.
ACCESSION   DAJTRC010000189 DAJTRC010000000
VERSION     DAJTRC010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4430)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4430)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4430
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.191"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(291..1148)
                     /gene="sitD"
                     /locus_tag="OO357_003964"
     CDS             complement(291..1148)
                     /gene="sitD"
                     /locus_tag="OO357_003964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitD"
                     /protein_id="HCR5888712.1"
                     /translation="MMALLLEPLQFTFMSHALLISLVVSIPCALLSVFLVLKGWALMG
                     DAMSHAVFPGIVLTWILGLPLATGAFVAGVFCAVATGYLKDNSRIKQDTVMGIVFSGM
                     FAAGLILYIAIKPDVHLDHILFGDMLGITIGDIIQTVIIAGLVTLVISVKWRDFLLFS
                     FDYQQAQVSGLHTRWLHYGLLCMVSLTIVATLKAVGIILSISLLIAPGAIAVLLTQRF
                     HIALLLATGISVIVSMTGVWLSFFIDSAPAPTIVVLFAVVFIMTFTVTSINARTKGNA
                     HTQDLLSPN"
     gene            complement(1145..2002)
                     /gene="sitC"
                     /locus_tag="OO357_003965"
     CDS             complement(1145..2002)
                     /gene="sitC"
                     /locus_tag="OO357_003965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter permease subunit
                     SitC"
                     /protein_id="HCR5888713.1"
                     /translation="MNVLLEPFSYEYMLNAMWVSAMVGGLCAFLSCYLMLKGWSLIGD
                     ALSHSIVPGVAGAYMLGLPFSLGAFFSGGLAAGSMLFLNQRTRLKEDAIIGLIFSSFF
                     GLGLFMVSLNPTSVNIQTIVLGNILAIDPADILQLTIIGILSIIVLFFKWKDLMVTFF
                     DENHARAIGLHPGRLKILFFTLLSVSTVAALQTVGAFLVICLVVTPGATAWLLTDRFP
                     RLLIIAVTIGSVTSFLGAWVSYFLDGATGGIIVVAQTLLFLLAFVFAPTHGLLANRRR
                     AHKALEDRS"
     gene            complement(1999..2826)
                     /gene="sitB"
                     /locus_tag="OO357_003966"
     CDS             complement(1999..2826)
                     /gene="sitB"
                     /locus_tag="OO357_003966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter ATP-binding
                     protein SitB"
                     /protein_id="HCR5888714.1"
                     /translation="MMQSAGIVVNDVTVTWRNGHTALRDASFTVPSGSIAALVGVNGS
                     GKSTLFKAIMGFVRLTSGKISVLGIPTRQALQKNLVAYVPQSEEVDWSFPVLVEDVVM
                     MGRYGHMGMLRIAKKRDRQIVTDALERVDMVDFRHRQIGELSGGQKKRVFLARAIAQQ
                     GDVILLDEPFTGVDVKTEAKIISLLRELRAEGKTMLVSTHNLGSVTTFCDYTVMVKGT
                     VLASGPTDTTFTAENLELAFSGVLRHVTLNGSEESIITDDERPFVAHRPSAVQREAR"
     gene            complement(2826..3740)
                     /gene="sitA"
                     /locus_tag="OO357_003967"
     CDS             complement(2826..3740)
                     /gene="sitA"
                     /locus_tag="OO357_003967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000949004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron/manganese ABC transporter substrate-binding
                     protein SitA"
                     /protein_id="HCR5888715.1"
                     /translation="MHSIKKVTMLLGCLALTCSIAFQASATEKFKVITTFTIIADMAK
                     NVAGDAAEVSSITKPGAEIHEYQPTPGDIKRAQGAQLILANGMNLELWFQRFYQHLNG
                     VPEVIVSSGVTPVGITEGPYEGKPNPHAWMSPDNALIYVDNIRDALIKYDPANAQTYQ
                     RNADTYKAKITQTLAPLRKQITELPENQRWMVTSEGAFSYLARDLGLKELYLWPINAD
                     QQGTPQQVRKVVDIVKKNHIPAVFSESTISDKPARQVARETGAHYGGVLYVDSLSTEN
                     GPVPTYIDLLKVTTSTLVQGIKAGKREK"
     gene            complement(4172..>4430)
                     /locus_tag="OO357_003968"
     CDS             complement(4172..>4430)
                     /locus_tag="OO357_003968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HCR5888716.1"
                     /translation="QKPYYSVSSLGTRWNAAVKRAGIRRRNPYHTRHTFACWLLTAGA
                     NPAFIASQMGHETAQMVYEIYGMWIDDMNDEQIAMLNARLS"
BASE COUNT         1189 a         1077 c         1061 g         1103 t
ORIGIN      
        1 gatgagattt atcgccgtca gcgggagctg aaggaagagc tgaatagtct gaaggatttg
       61 aattcggtta ggaattttat cgtggaataa cagaagctgc ggcacgtcgt atgcaggaac
      121 gtgccgcggt tggctggtaa actttcgata gtgcgagtat tgaatgattt ctaggcgtta
      181 tcgattttac gtattttttg catgatagaa ttcgtacctc ctcccaacga ccttccatgg
      241 ctttccggac ttctgtaggc tggcggacga tgctgtcctc ggatgttgtg ctaattgggt
      301 gataacagat cctgtgtatg ggcgtttccc tttgtgcgtg cgttgatgct ggtaacggta
      361 aacgtcatga tgaacacgac cgcgaataag acgacaatcg tcggagctgg tgcgctgtcg
      421 ataaaaaagg acagccagac accagtcatt gacactatta ccgatatgcc agtcgccagt
      481 aggagcgcaa tatgaaagcg ttgtgtcagg agtactgcaa tggcgccagg cgcaatgagc
      541 agagaaatag agagaatgat acctactgct ttcagcgtcg ccacaatggt cagggaaacc
      601 atgcacagca gcccataatg cagccatctc gtatgcaaac cgcttacctg cgcctgttga
      661 taatcaaagc tgaacagcaa aaaatcccgc cattttacgc taataacaag tgtaaccagc
      721 ccggcaataa tcaccgtctg gattatgtcg ccgatggtta tcccgagcat gtcgccgaaa
      781 agaatatggt caagatgtac gtctggttta attgcaatat acaagatcag acctgcggca
      841 aacatgccag aaaaaacaat ccccataact gtatcttgct tgattcggct gttgtctttc
      901 aggtatcccg tcgccaccgc gcagaatact ccggcgacga atgcccctgt agccagaggt
      961 aaccccagaa tccaggtaag cacgatgcca ggaaaaaccg catgactcat cgcgtcaccc
     1021 atcagtgccc aacctttcag gaccagaaag accgacagca gcgcacaggg tatggaaact
     1081 accagtgaaa tcagcagtgc gtgactcata aaggtgaact gcagtggctc cagtaataat
     1141 gccatcatga gcgatcctcc agcgctttat gcgcacgccg acgatttgcc aaaagtccat
     1201 gggtgggcgc aaatacaaat gctaaaagaa acagcagtgt ctgcgccacc acgatgattc
     1261 cacctgtcgc gccgtccaga aagtaactga cccacgcgcc gaggaaactg gttacactgc
     1321 caatagttac agcaattatg agcaggcggg gaaaacgatc ggttaacaac catgctgttg
     1381 caccgggagt caccaccaga cagatcacca gaaaggcacc aaccgtttgc agcgccgcaa
     1441 cggtcgaaac cgacagcagg gtgaagaaga ggatttttaa tctgcccgga tgtaatccaa
     1501 tagcacgggc gtgattctcg tcgaaaaagg tcaccataag atctttccat ttgaaaaaaa
     1561 gcacgatgat cgagagaatg ccaatgatag tgagttgcag gatatccgcc ggatcgattg
     1621 ccagaatatt gcccaggacg atggtctgaa tgttcacgga tgtcgggttc aatgacacca
     1681 taaacagccc caggccaaaa aaggacgaga agatcaggcc gatgatggca tcttctttta
     1741 agcgggtgcg ttggttaaga aacagcatgc tgcccgctgc cagtccgccg gaaaagaagg
     1801 ctcccagaga aaacggaagc cccagcatat atgcccccgc aacacccggg acaatagagt
     1861 gcgacagcgc atcgccaatc agcgaccagc cttttagcat cagatagcat gacaggaaag
     1921 cgcagagacc gccgaccatt gccgagaccc acatcgcatt gagcatatat tcatagctga
     1981 agggttccag tagcacgttc atcttgcttc cctctgcacc gctgacggtc gatgtgccac
     2041 aaaagggcgt tcatcgtcag taataatgct ttcttccgag ccgttaagtg tgacatggcg
     2101 cagtacgccg ctaaaagcca gctccaggtt ctcggctgta aaagtggtgt ctgtcggccc
     2161 gcttgccagg acggtgcctt tgaccatgac cgtataatcg caaaatgtcg tgactgagcc
     2221 gagattgtgc gttgagacaa gcatcgtttt gccttccgcg cgtaactccc tcaacaggct
     2281 gatgattttc gcttcggttt tcacatcaac gccggtaaaa ggttcatcca gcaggatcac
     2341 atcaccctgc tgtgcaattg ctctcgccag aaagacgcgt ttcttttgtc caccggaaag
     2401 ctcgccgatt tgacgatggc gaaaatccac catatcgaca cgttccagcg catcggtgac
     2461 aatctgacga tccctctttt tggctatacg caacataccc atatgcccat agcggcccat
     2521 catcaccaca tcttccacca gtacaggaaa tgaccagtca acctcttctg actgagggac
     2581 gtaggcaacc aggtttttct gtagcgcctg tcgcgtggga atacccagaa cagatatctt
     2641 tccgctggtc agacgcacaa atcccataat cgctttaaac aacgtcgatt ttccggaacc
     2701 gtttacccca accagagcgg caatagatcc acttggtact gtgaaggatg catcgcgcag
     2761 tgctgtgtgc ccgttacgcc aggtgacagt aacatcatta acgacaatgc ctgcagattg
     2821 catcattatt tctccctttt cccggcttta attccctgta ccagcgtact ggtagtaacc
     2881 ttaagaaggt cgatatacgt aggtaccggg ccgttttctg tgctcaggga atcgacatag
     2941 agcacaccgc cgtagtgtgc gccggtttca cgcgcaacct gacgcgctgg tttatcggaa
     3001 atcgtactct cgctaaagac tgccgggata tgatttttct taactatatc aacaacctta
     3061 cgtacctgct gcggtgttcc ttgttgatcg gcattaatcg gccacagata aagctctttt
     3121 agccccaaat cgcgtgcgag ataagaaaaa gccccttcac tggtgaccat ccatcgctga
     3181 ttctcaggga gttccgtaat ctgcttacgc aggggggcaa gggtttgggt aatcttggct
     3241 ttataagtat cggcattacg ttggtaggtt tgtgcatttg ccggatcgta ttttatcaac
     3301 gcatcacgaa tattatcgac gtaaatcaga gcattatctg gcgacatcca tgcatgggga
     3361 ttaggtttgc cctcataggg tccttcggtg atccctactg gcgtcacacc cgaagagaca
     3421 attacttctg gaaccccatt gagatgctgg taaaagcgtt ggaaccacaa ttccagattc
     3481 ataccattgg cgagaatcag ttgtgccccc tgcgcacgtt taatatcgcc aggggtaggc
     3541 tgatactcat gaatttctgc accaggcttg gttatggatg agacttctgc agcatctcca
     3601 gccacgtttt tggccatatc cgcgatgatg gtaaatgttg taatgacctt gaatttttca
     3661 gttgcacttg cctgaaatgc gatcgagcag gtgagggcga ggcaccccaa gagcatggtt
     3721 acttttttta ttgagtgcat aatttagtac ctatagtgag caaaattaga acaaacatag
     3781 caacggctat gttttatagc ataacctata caaaatgaaa ataattatca ttaacagctt
     3841 tgaggcctaa aacagggatt aggttgtttc ctgctggcga catgatattg aggaaaaaag
     3901 tagagcagct attaggcttt gaaatgactt tatgaggaaa attgttgatg gcaaaaatgg
     3961 ggcaaaaaag agtgggttat cgtagcttat tgttgtcgct gataatgttt aacatattga
     4021 aaaataaata aaatacttat gagtcagata gttgtgattt ttgcccttac ttgttcaggt
     4081 tgtattgttc tttctttgta atttgttgat tttcttgcat tatttcagtt ctctggtact
     4141 aaatggggca aattgggggc aaactttgca actacgataa ccgcgcattc aacatggcta
     4201 tctgttcgtc gttcatgtca tcaatccaca taccgtaaat ttcatacacc atttgcgcag
     4261 tttcatgccc catctggctg gctataaatg ccgggttcgc tcctgccgtc aacagccagc
     4321 aggcaaaagt atgtcgcgta tggtacggat tacggcggcg aataccagca cgttttactg
     4381 ctgcattcca tcttgtaccc aaactgctta ccgagtaata aggtttctgt
//
LOCUS       DAJTRC010000190         4395 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.193, whole genome shotgun sequence.
ACCESSION   DAJTRC010000190 DAJTRC010000000
VERSION     DAJTRC010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.193"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(85..669)
                     /gene="kptA"
                     /locus_tag="OO357_003969"
     CDS             complement(85..669)
                     /gene="kptA"
                     /locus_tag="OO357_003969"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2'-phosphotransferase"
                     /protein_id="HCR5888717.1"
                     /translation="MAKYNEKELADTSKFLSFVLRHKPEAIGIVLDREGWADINKLIL
                     CAQKAGKRLTRALLDTVVATSDKKRFSYSSDGRCIRAVQGHSTSQVAISFAEKTPPQF
                     LYHGTASRFLDEIKKQGLIAGDRHYVHLSADEATARKVGARHGSPVVLTVKAQEMAKR
                     GIPFWQAENGVWLTSTVAVEFLEWSFMPTGVQKQ"
     gene            919..1602
                     /locus_tag="OO357_003970"
     CDS             919..1602
                     /locus_tag="OO357_003970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418750.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiH family protein"
                     /protein_id="HCR5888718.1"
                     /translation="MTQQGDAVAGELATEKVGIKGYLAFFLTIIFFPGVFSGTDSWWR
                     VFDFSVLNGSFGQLPGANGATTSFRGVGGAGAKDGFLFALELAPSVILSLGIISITDG
                     LGGLRAAQQLMTPVLKPLLGIPGICSLALIANLQNTDAAAGMTKELAQEGEITERDKV
                     IFAAYQTSGSAIITNYFSSGVAVFAFLGTSVIVPLAVILVFKFVGANILRVWLNFEER
                     RNPTQGAQA"
     gene            1599..2060
                     /locus_tag="OO357_003971"
     CDS             1599..2060
                     /locus_tag="OO357_003971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjiG family protein"
                     /protein_id="HCR5888719.1"
                     /translation="MTTQVRKNVMDMFIDGARRGFTIATTNLLPNVVMAFVIIQALKI
                     TGLLDWVGHICEPVMALWGLPGEAATVLLAALMSMGGAVGVAASLATADALTGHDVTV
                     LLPAMYLMGNPVQNVGRCLGTAEVNAKYYPHIITVCVINALLSIWVMQLIV"
     gene            2073..3245
                     /gene="iadA"
                     /locus_tag="OO357_003972"
     CDS             2073..3245
                     /gene="iadA"
                     /locus_tag="OO357_003972"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HCR5888720.1"
                     /translation="MIDYTAAGFTLLQGAHLYAPEDRGICDVLVANGKIIAVASNIPS
                     DIVPNCTVVDLSGQILCPGFIDQHVHLIGGGGEAGPTTRTPEVALSRLTEAGVTSVVG
                     LLGTDSISRHPESLLAKTRALNEEGISAWMLTGAYHVPSRTITGSVEKDVAIIDRVIG
                     VKCAISDHRSAAPDVYHLANMAAESRVGGLLGGKPGVTVFHMGDSKKALKPIYDLLEN
                     CDVPISKLLPTHVNRNVPLFEQALEFARKGGTIDITSSIDEPVAPAEGIARAVQAGIP
                     LARVTLSSDGNGSQPFFDDEGNLTHIGVAGFETLLETVQVLVKDYDFSISDALRPLTS
                     SVAGFLNMSGKGEILPGNDADLLVMTPELRIEQVYARGKLMVKDGKACVKGTFETA"
     gene            3310..4221
                     /gene="hypT"
                     /locus_tag="OO357_003973"
                     /gene_synonym="qseD"
     CDS             3310..4221
                     /gene="hypT"
                     /locus_tag="OO357_003973"
                     /gene_synonym="qseD"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypochlorite stress DNA-binding transcriptional
                     regulator HypT"
                     /protein_id="HCR5888721.1"
                     /translation="MDDCGAVLHNIETKWLYDFLTLEKCRNFSQAAVSRNVSQPAFSR
                     RIRALEQAIGVELFNRQVTPLQLSEQGKIFHSQIRHLLQQLESNLAELRGGSDYAQRK
                     IKIAAAHSLSLGLLPSIISQMPPLFTWAIEAIDVDEAVDKLREGQSDCIFSFHDEDLL
                     EAPFDHIRLFESQLFPVCASDEHGEALFNLAQPHFPLLNYSRNSYMVRLINRTLTRHS
                     ELSFSTFFVSSMSELLKQVALDGCGIAWLPEYAIQQEIRSGQLVVLNRDEQVIPIQAY
                     AYRMNTRMNPVAERFWRELRELEIVLS"
     gene            complement(4214..>4395)
                     /gene="iraD"
                     /locus_tag="OO357_003974"
     CDS             complement(4214..>4395)
                     /gene="iraD"
                     /locus_tag="OO357_003974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313312.2"
                     /note="inhibitor of RssB activity in response to DNA
                     damage; blocks degradation of stationary phase sigma
                     factor RpoS; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="anti-adapter protein IraD"
                     /protein_id="HCR5888722.1"
                     /translation="ALLFHEPRIAALQVNLKEAYCHTLAISLEIMLYHDDEPLTFDLV
                     WDNGGWRSATLENVS"
BASE COUNT         1005 a         1174 c         1120 g         1096 t
ORIGIN      
        1 tatcccttca gactgataac aaacgctaca ttgccggatg tgctgcgctt accaggccta
       61 tataacccct gcaatatatt gaatttattg tttttgtacc ccagtcggca taaacgacca
      121 ctcaagaaac tcaacagcaa ccgttgatgt cagccagacc ccgttttccg cttgccagaa
      181 agggatgcct cgtttcgcca tttcctgtgc tttgacggtt aagacaaccg gcgatccgtg
      241 tcttgctccc actttgcgtg ccgttgcttc atcagcagag agatgcacgt aatgacgatc
      301 ccctgcaatc agcccctgtt tttttatctc gtcaaggaaa cggcttgccg taccgtggta
      361 gagaaactgt ggcggtgttt tttcggcaaa ggaaatagcg acctgcgaag tcgaatgccc
      421 ctgaaccgcg cggatgcacc taccatcact ggaataacta aaacgctttt tatcgctggt
      481 tgcgaccacg gtatccagca atgcacgggt taatcgcttg cccgcttttt gggcacaaag
      541 tatgagctta ttgatatccg cccatccttc acggtccagt acgataccaa tcgcctccgg
      601 tttgtgtcgg aggacaaaac ttaaaaactt gctcgtatcc gccagctctt tttcgttgta
      661 ctttgccatc gcgttttcgt ccctggattt tctgttttta tttactaatg tcattgtcac
      721 tgggtttatg aattttctgc aactcaatcc tgcgcagaac cttttccaca cagcgtaatt
      781 tttcccctat gcaaaaaacg catagccaga aacatacttg cattttcccc atacccacat
      841 ggctgataag gtttttcagt tccaggaatt ttcctggctc aagcaacaca caaacacaaa
      901 aaaacaatgg ggattgttat gacacaacaa ggggatgctg tagccgggga gctggcgaca
      961 gaaaaagtgg gcattaaagg gtatctggct ttttttctca ccataatctt ttttcccggt
     1021 gttttttccg gaacagacag ctggtggcgc gtttttgatt tttccgttct gaacggatcg
     1081 tttgggcaac tgcctggtgc gaatggagcc accacctcat ttcgcggggt gggtggcgcg
     1141 ggggctaaag atggctttct ctttgcgctg gagctggcac cgtcagtcat tctctctctg
     1201 gggattattt caattaccga tggtcttggc ggattacgcg ccgcgcagca gttaatgaca
     1261 cctgtgctaa aaccgttgtt gggcattcca gggatctgct cgctggcact gatcgctaac
     1321 ctgcaaaaca cggatgccgc cgccggtatg accaaagagc tggcgcagga aggtgaaatt
     1381 accgaacgcg ataaagtgat tttcgccgca tatcaaacca gcggtagcgc catcatcacc
     1441 aactacttct cttccggcgt cgcggtgttt gcctttctgg gcacgtccgt aatcgtacct
     1501 ttagccgtca tcctggtgtt taaatttgtc ggagccaaca ttttgcgcgt ctggcttaac
     1561 ttcgaagaac gtcgcaaccc aacgcaagga gcgcaagcat gacaactcag gtccgcaaaa
     1621 acgtcatgga tatgtttatc gatggcgcgc gccgtggctt tactatcgcc acgaccaacc
     1681 tgctgccaaa cgtggtgatg gcgtttgtca tcattcaggc gctgaaaatt accggcctgc
     1741 tcgactgggt ggggcatatc tgtgaacccg taatggcgct gtggggatta cccggtgaag
     1801 cggcaaccgt ccttttagcc gcattaatga gtatgggcgg cgcagtcggt gttgccgcca
     1861 gtctggcaac cgctgacgca ttaaccggcc acgatgtcac cgttttactg cccgcaatgt
     1921 atttaatggg caacccggtg caaaatgtcg gtcgctgcct ggggacagcc gaagtgaatg
     1981 ccaaatatta cccgcatatc attacggtct gcgtgattaa cgcattgctg tcgatctggg
     2041 ttatgcagct tattgtttaa caaggagtta ccatgattga ttataccgca gccggtttta
     2101 ccctgctgca gggagcgcat ttgtatgcgc cggaagatcg gggaatttgc gatgtcctcg
     2161 tcgctaacgg caaaattatc gccgttgcca gcaatatccc ttctgacatt gtaccgaact
     2221 gcacggttgt cgatctcagc gggcagatcc tctgcccagg ttttattgat caacacgtcc
     2281 atttgattgg cggtggcggc gaagcaggtc ccacgacgcg cacgccggaa gtggcgctaa
     2341 gtcgcctgac ggaagcgggc gtcacgtcag tggttggtct gctgggcacc gactctatct
     2401 ctcgccaccc ggaatccctg ctcgccaaga cccgtgcgct caatgaagaa ggcatcagcg
     2461 cctggatgct gaccggcgct tatcatgtcc cttcccgcac tattacgggt tccgtggaaa
     2521 aagacgtggc gattatcgat cgtgtgattg gcgtgaaatg cgccatctct gatcaccgtt
     2581 ctgccgcacc ggacgtttat cacctggcta atatggcggc agaatcccgc gttggcggtt
     2641 tgctcggcgg taaacctggc gtcaccgtgt tccacatggg cgacagtaaa aaggcgttaa
     2701 agcctattta tgacctgctg gaaaactgcg atgtgccgat cagcaagctg ctgccgaccc
     2761 acgttaaccg caacgtaccg ttgtttgagc aggcgctgga gttcgcgcgc aaaggcggca
     2821 ccatcgatat caccagcagc attgacgaac cggtcgcccc tgccgaaggt attgcccgcg
     2881 ccgttcaggc gggtattccg ctggcacgcg tcaccctcag ctccgacggc aacggtagcc
     2941 agccgttctt cgatgacgaa gggaatttaa cccatatcgg tgttgcaggt tttgaaacgt
     3001 tgctggaaac cgtgcaggtg ctggtcaaag actatgattt cagtatcagc gatgccctgc
     3061 gcccgctcac cagtagcgta gccggtttcc ttaacatgag cgggaaaggc gaaattctgc
     3121 caggcaatga cgcagactta ctggtcatga cgccagaatt gcgcattgag caggtatacg
     3181 ctcgcggcaa actgatggtc aaagacggca aagcctgcgt gaaaggaacg tttgaaacgg
     3241 cttaaacggt tgtcagccgc cctaatggac gtagaatgcc cccaagggcg gctgacagag
     3301 taaaacgtaa tggatgactg tggtgcggtt ttgcataata ttgaaaccaa atggctttat
     3361 gattttctga ctctggaaaa atgccgcaat ttttcccagg cggcagtcag tcgcaacgtc
     3421 tcgcaaccgg cattcagccg ccgcatccgt gcactggaac aggcgattgg cgttgaattg
     3481 tttaaccgcc aggtgacgcc actgcaactc tcagaacaag ggaaaatctt ccattcgcag
     3541 atccgccatc tgttgcaaca gttagaaagc aaccttgcag agctgcgtgg cggcagcgat
     3601 tacgcgcaac gtaaaatcaa gatagccgct gcacactctc tttccctcgg tctgttaccg
     3661 tccattatca gccagatgcc gccgctcttt acctgggcaa ttgaagccat tgatgtcgat
     3721 gaagcggtcg ataaactgcg tgaagggcaa agtgactgta ttttttcctt tcacgacgaa
     3781 gatttgctgg aagcgccgtt tgaccacatt cgcttatttg aatctcaatt gttccctgtc
     3841 tgcgccagtg acgaacacgg agaagcactt tttaacctcg cgcagccaca ctttccgtta
     3901 ctgaattaca gccgcaactc ctacatggtg cgattgatta atcgcaccct gacgcgccac
     3961 agtgagttaa gtttcagcac cttttttgtc tcttcgatga gcgagctttt aaagcaggtt
     4021 gccctcgacg gctgtgggat tgcctggctg ccggagtatg ccatccaaca agaaattcgc
     4081 agcgggcagc tcgttgtgct gaatcgggac gaacaggtga tcccgattca ggcctacgca
     4141 taccggatga atacccgcat gaatccagtt gccgaacgct tctggcgtga actgcgcgag
     4201 ctggagattg tgcttagctg acattctcaa gcgtcgcact gcgccaaccg ccattatccc
     4261 agaccagatc aaacgttagc ggttcatcgt cgtggtacag cattatctca aggctgatcg
     4321 ccagagtgtg gcagtacgct tctttcaaat taacctgcaa cgccgcaatc cgtggctcat
     4381 gaaataacag tgcag
//
LOCUS       DAJTRC010000191         4276 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.195, whole genome shotgun sequence.
ACCESSION   DAJTRC010000191 DAJTRC010000000
VERSION     DAJTRC010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4276
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.195"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(1..1265)
                     /gene="ynjE"
                     /locus_tag="OO357_003975"
                     /pseudo
     CDS             complement(1..1265)
                     /gene="ynjE"
                     /locus_tag="OO357_003975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310490.2"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="thiosulfate sulfurtransferase YnjE"
     gene            complement(1332..1985)
                     /locus_tag="OO357_003976"
     CDS             complement(1332..1985)
                     /locus_tag="OO357_003976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707358.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5888723.1"
                     /translation="MLCVKNVSLRLPESRLLTNVNFTVDKGDIVTLMGPSGCGKSTLF
                     SWMIGALAGQFSCTGELWLNEQRIDMLPTAQRQIGILFQDALLFDQFSVGQNLLLALP
                     ATLKGNARRNAVNDALERSGLDGAFHQDPATLSGGQRARVALLRALLAQPKALLLDEP
                     FSRLDVALRDNFRQWVFSEVRALAIPVVQVTHDLQDVPADSSVLDMAQWSENYNKLR"
     gene            complement(1985..3456)
                     /locus_tag="OO357_003977"
                     /pseudo
     CDS             complement(1985..3456)
                     /locus_tag="OO357_003977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416269.4"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
     gene            complement(3429..4276)
                     /locus_tag="OO357_003978"
                     /pseudo
     CDS             complement(3429..4276)
                     /locus_tag="OO357_003978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416268.2"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT         1019 a         1201 c         1133 g          923 t
ORIGIN      
        1 ttttttggat cgctgctcca ttcgtaccag ccgccgtcat acacggaaac attcttccaa
       61 cccatggcgc gtgcgtacat aaaggtttcg gacgcgcgcc agccggtgcc gcagtagaat
      121 gaaacttgct gatctggttt gatattccat gctttccaca tagcggtaat atcatcggcg
      181 ctacgcatgg tgccatccgg gttatagaaa tcttccatat gcgtcgagtc gctaccagcg
      241 tgtccccaac gtgctccggc tatttcacct tttggtttaa tatagctgta accgctggtc
      301 gtaccgataa attctggcca cgaacgaatg ctcaccagcg atgcatcctg gcgatgcagc
      361 agtccacggg cctgttccat atcgagcatc aactgcggtt gtgccgggat cttcacgccg
      421 aaatccggtt ccgctttcac tttcggtggc gttcctcgct caacaggcag tcccgcgtcg
      481 gaccaggttt gccagccgcc atccagcaga cgcacatctt tcacgcctgc gtagagcata
      541 atttgcgcca cacgcgctcc agcgtatacg tcacgcccgt acagaatgac cgtggtgtca
      601 tggcgaatgc cgtgttttgc cagcatcgct tttagttgtt catcagaaac tttgttccac
      661 agcggttcgc tttccacttc gttggtatcg atgtagtcag cgccaggaat atggctgata
      721 aggtaaagct taggagcgcc ctaggccgct tcaatgactt tccagtcacc gacaggtttc
      781 gccgtaacct ctttaccttg ttgcaggtca tgcagccatt gcggataaac cagctgctca
      841 aagtgcggca gtttttgtag acgggaaggt tcgcttagcg cgtcactcag aatggagata
      901 tgcgtaaaac ctgctttttg cagtcgcgtt ttgacggcgt caacatcttt gccattaccg
      961 tacagcgcca ccggggcatc ggctttcagg ttatgttgct tgatccacgc gttgagctgt
     1021 tcggtgctca ttttgtccag ccagctagca gagaggttta aggcggcagg ttcatgacca
     1081 gaagggccat ttaaggtttg tggccagccg ttataaaacg cgctggggcg agtatctatc
     1141 gctttgccat tttgttgctg aagttggtca agtgtaagag gcttcgccag ttcagcggcc
     1201 cacgaagaag cgcaagccag ccctaaagcc attgccagcg cggtcatttg agaaacacgt
     1261 ttcatcgaaa atcccgacat tgtagttttg agtgcgccat tctgaactga cgcattgaga
     1321 aaaactttgc gttatcgcag tttgttgtaa ttttctgacc actgcgccat atccagaaca
     1381 gaactatcag caggaacatc ctggagatcg tgcgttacct gaacgacggg gatcgccagg
     1441 gcgcgaactt cgctgaacac ccactggcga aaattatcgc gcagagccac atcaagacgg
     1501 ctgaatggct catccaggag taacgctttt ggctgggcga gaagggcgcg tagtagagca
     1561 acgcgcgctc gctgaccgcc agacaaagtg gcaggatcct gatggaaagc tccgtcaagg
     1621 cctgaacgct caagtgcatc attcacggca ttacgtcggg cattcccctt aagtgtcgcc
     1681 ggtagcgcca gcagtaaatt ttgcccgacg ctgaactggt caaataacag tgcatcctga
     1741 aaaagaatgc caatctgacg ctgtgcggtg ggtagcatgt caatccgttg ctcattgagc
     1801 catagctcac ctgtacaaga aaactgtccg gccagtgcac caatcatcca tgaaaacaga
     1861 gtggattttc cacagccaga cggccccatt aacgtgacga tgtcaccttt atcaaccgta
     1921 aagttaacgt ttgtcagcaa gcggctttct ggtaaacgta gcgaaacatt tttcacgcag
     1981 agcattagcg gattccttgt ctgacataac ctacccattt tgcgactaac gcggtcaggg
     2041 caaaaataat aagcggtaac aacagttgcc ataacgcctg ggcggcgaga ataccgttgc
     2101 tgccgccgct gcttaatgcc accgcctcag tggtgagcgt cggaaaacgc cccgcgccca
     2161 gccacagcgt cggcatatac tgcgcaatac tgactgaaaa tcccaccgcg aaggcaatca
     2221 gcacagggcg caacatgagt gggcatttca cgtaaaagaa gattttggcc cgcgaccagc
     2281 ccagtgtttg cgcaatcaaa attaaccgtg aatcaatgcg ctgccaggcg ggttgcagga
     2341 taaacagcat ccacggcatt acccacagca gatgccccca gaccaccgcc gtccagcttc
     2401 catccaggtt cagccatagc gccagcgtgt actggcccgc caccagtggc agagcgggca
     2461 atagaatggg caaccatagc cacaactggc gacgctgtgc cccccattcc agccacagca
     2521 gtaacaggag caaagcgatg aatgttgcta ccagccccat tgtcagactg ttgatgagtg
     2581 cttcactatt gatcgtcgac tgatccgcga ggatcgccag cagaaccaca cacagcacac
     2641 cggttaaggg taaaaaactc gccagcgtag tgcctggtaa taaaggtgtg gcaggcttgc
     2701 gaatgccatc tacgcgggga atagtgcgcc gccagctgcg ccaaaacgta ggcggcgagt
     2761 aacagcatca gcagcagact ggcgagtgcg cctttcgttt gttgatccgc gtcgccctga
     2821 gttaaccact gccagctaat tactgccagc gtcggtggat tacctggccc aagaataatc
     2881 gccacatcca cgaccgaaag cgaccaggca acaatcgcca gcatcgccat tgccagcggc
     2941 tgtagcccag cgaatccagc acaatgacct gctgcaacag ccgtttttcg ctcaatactg
     3001 ccactaagat ccacagcaga aatgcgcttt ctttcactgc aagggtgagg cccagaccga
     3061 tccaaatcgg tccattggcg gagtgaaata cgggaaatag tcatatagca gccctccgtc
     3121 agcaaagagc agcagggcgc tggtggcaaa agccacatgg ggaatggcga gcagccacgg
     3181 cagacgggcg cacatacgct gccatttcgg cccaggccat agcgccacaa tcaccagcag
     3241 ggcaatcaac aatgccccga ccgccgcgat ggttgtcgac accagcgttg ccagtaatgc
     3301 ctgcggtaac tgtggatcgg caaataacgc ctgccagcgt gtcaacgaca acgcaggcga
     3361 tatcagcgtg agagctgccg ggatcagcgg tgcataaatc accgccacca tcgcccacag
     3421 caaaaagatt aatgcgtacc gtaacggcgt aaccattctt gttccagtgc attcacccat
     3481 cctgcgtgcg gttcagccag taccggcggc agatcctgcg gcattcttga ttgcaatatt
     3541 tcgcgctgcc cgtcaggcag tttttgcgga tcgagaacag aaggatcgcc ccagacaacg
     3601 ggatctgctt tacgcagttg cgcatcgggt gagagcagga aattggcaac taccttcgcc
     3661 gcagcactgg cattcgcgtt ggctggaatg gtgacgaaat gcacgttgcc aatcatcccc
     3721 tcgcgaaagc caaaactgta actgcttgcc ggcaaatcgc cgctggcgat tttttgctgc
     3781 gcatgtgcgg ggttaaaggt cagcgacagg cgcaatgtgc cggctttcag cagagcatcc
     3841 atccgcgcgg gcgaaggcgg gaaatccttt ccttcgcgcc acaaatacgg atgcagtgca
     3901 tcaagatatt gctacaaggg cgcagtgaca cgggcgaaag tcgcatcgtc cggcgcttct
     3961 tttaatgcgg cgggatctgg cgtcagcata atcagcaact gttcaagaaa cgccgtgccg
     4021 gtaaagtccg gtgggcgcgg ataagtaacc gtgccgggat tggctttagc aaactccagt
     4081 aaggcttgcg gcgtttgtgg tggctgtggc gtaacatcgc ggcgggcgat aaacgtcagt
     4141 tgtgcgccgc cccagggcga ttccgcacct tgtgtcggca ctgaaaaatc tccccgcacc
     4201 ggcagctgtg tgtcgacata gcgccagttg ggcagagtct ccgcccagcc cgtttgtaat
     4261 aaattggcct ctttta
//
LOCUS       DAJTRC010000192         4249 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.196, whole genome shotgun sequence.
ACCESSION   DAJTRC010000192 DAJTRC010000000
VERSION     DAJTRC010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4249)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4249)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4249
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.196"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            241..1680
                     /gene="aldA"
                     /locus_tag="OO357_003979"
     CDS             241..1680
                     /gene="aldA"
                     /locus_tag="OO357_003979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HCR5888724.1"
                     /translation="MSVPVQHPMYIDGQFVTWRGDAWIDVVNPATEAVISRIPDGQAE
                     DARKAIDAAERAQPEWEALPAIERASWLCKISAGIRERASEISALIVEEGGKIQQLAE
                     VEVAFTADYIDYMAEWARRYEGEIIQSDRPGENILLFKRALGVTTGILPWNFPFFLIA
                     RKMAPALLTGNTIVIKPSEFTPNNAIAFAKIVDEIGLPRGVFNLVLGRGETVGQELAG
                     NPKVAMVSMTGSVSAGEKIMATAAKNITKVCLELGGKAPAIVMDDADLELAVKAIVDS
                     RVINSGQVCNCAERVYVQKGIYDQFVNLLGEALQAVQFGNPAERNDIAMGPLINAAAL
                     ERVEQKVARAVEEGARVALGGKAVEGKGYYYPPTLLLDVRQEMSIMHEETFGPVLPVV
                     AFDTLEDAISMANDSDYGLTSSIYTQNLNVAMKAIKGLKFGETYINRENFEAMQGFHA
                     GWRKSGIGGADGKHGLHEYLQTQVVYLQS"
     gene            complement(1722..2722)
                     /gene="gap"
                     /locus_tag="OO357_003980"
                     /pseudo
     CDS             complement(1722..2722)
                     /gene="gap"
                     /locus_tag="OO357_003980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310049.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
     gene            2911..3441
                     /gene="cybB"
                     /locus_tag="OO357_003981"
     CDS             2911..3441
                     /gene="cybB"
                     /locus_tag="OO357_003981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415935.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b561"
                     /protein_id="HCR5888725.1"
                     /translation="MENKYSRLQISIHWLVFLLVIAAYCAMEFRGFFPRSDRPLINMI
                     HVSCGISILVLMVVRLLLRLKYPTPPIIPKPKPMMTGLAHLGHLVIYLLFIALPVIGL
                     VMMYNRGNPWFAFGLTMPYASEANFERVDSLKSWHETLANLGYFVIGLHAAAALAHHY
                     FWKDNTLLRMMPRKRS"
     gene            3686..3859
                     /locus_tag="OO357_003982"
     CDS             3686..3859
                     /locus_tag="OO357_003982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888726.1"
                     /translation="MKKLALILFMGTLVSFYADAGRKPCSGSKGGISHCTAGGKFVCN
                     DGSISASKKTCTN"
     gene            complement(3931..4080)
                     /gene="hokB"
                     /locus_tag="OO357_003983"
     CDS             complement(3931..4080)
                     /gene="hokB"
                     /locus_tag="OO357_003983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system toxin HokB"
                     /protein_id="HCR5888727.1"
                     /translation="MKRNPLVVCLLIICITILAFTLLTRQTLYELRFRDGDKEVAALM
                     ACTSR"
BASE COUNT         1084 a          963 c         1069 g         1133 t
ORIGIN      
        1 atgcggtgct catcgaggcg atggaaagtc gctcgttacg ttaaaaattg cccgtttgtg
       61 aaccacttgt ttgcaaacgg gcatgactcc tgacttttat ttctgccttt tattcctttt
      121 acacttgttt ttatgaagcc ctccacagac ttgtcctttc ccgatgccgt ctctctgatg
      181 attgatgtta attaacaata tattcaccga aaacaaacat ataaatcaca agagtcgccc
      241 atgtcagtac ccgttcaaca tcctatgtat atcgatggac agtttgttac ctggcgtgga
      301 gacgcatgga ttgatgtggt aaaccctgct acagaggctg tcatttcccg catacccgat
      361 ggtcaggccg aggatgcccg taaggcaatc gatgcagcag aacgtgcaca accagaatgg
      421 gaagcgttgc ctgctattga acgcgccagt tggttgtgca aaatctccgc cgggatccgc
      481 gaacgcgcca gtgaaatcag tgcgctgatt gttgaagaag ggggcaagat ccagcagcta
      541 gctgaagtcg aagtggcttt tactgccgac tatatcgatt acatggcgga gtgggcacgg
      601 cgttacgagg gcgagattat tcaaagcgat cgtccgggag aaaatattct tctgtttaaa
      661 cgtgcgcttg gtgtgactac cggcattctg ccgtggaact ttccgttctt cctcattgcc
      721 cgcaaaatgg ctcccgctct tttgaccggt aataccatcg tcattaaacc cagtgaattt
      781 acgccaaaca atgcgattgc attcgccaaa atcgtcgatg aaataggcct tccgcgcggc
      841 gtgtttaacc ttgtactggg gcgtggtgaa accgttgggc aagaactggc gggtaaccca
      901 aaggtcgcaa tggtcagtat gacaggcagc gtctctgcag gtgagaagat catggcgact
      961 gcggcgaaaa acatcaccaa agtgtgtctg gaattgggtg gtaaagcacc ggctatcgta
     1021 atggacgatg ccgatcttga actggcagtc aaagccatcg ttgattcacg cgtcattaat
     1081 agtgggcaag tgtgtaactg tgcagaacgt gtttatgtac agaaaggcat ttatgatcag
     1141 ttcgtcaatc tgctgggtga agcgttgcag gcggttcaat ttggtaaccc cgctgaacgc
     1201 aacgacattg cgatggggcc gttgattaac gccgcggcgc tggaaagggt tgagcaaaaa
     1261 gtggcgcgcg cagtagaaga aggggcgaga gtggcgttgg gtggcaaagc ggtagagggg
     1321 aaaggatatt attatccgcc gacattgctg ctggatgttc gccaggaaat gtcgattatg
     1381 catgaggaaa cctttggccc ggtgctgcca gttgtcgcat ttgacacgct ggaagatgct
     1441 atctcaatgg ctaatgacag tgattacggc ctgacctcat caatctatac ccaaaatctg
     1501 aacgtcgcga tgaaagccat taaagggctg aagtttggtg aaacttacat caaccgtgaa
     1561 aacttcgaag ctatgcaagg tttccacgcc ggatggcgta aatccggtat tggcggcgca
     1621 gatggtaaac atggcctgca tgaatatctg cagacccagg tggtttattt acagtcttaa
     1681 tgagtgaaag aggcggaggt tttttcctcc gcctgtgcgc gtcagagttt agcgaatttt
     1741 tcgagggtgc gaataagctg cgtgacgaag ccatattcgt tatcgtacca ggcgaccgtt
     1801 ttcaccagtt gtaaatcgcc cacggcggta atttccgttt gcgtggcatc aaacactgaa
     1861 ccgaaatggc tgccaatgat atcggaagag actatttctt catcggtata accaaatgac
     1921 tcgttattgg tggttgcttg tttaagtgcg ttattcacct cttcggcagt cacttttttc
     1981 cgagaatgga caccagctca gtgaccgaac ctgttttcac cggcacgcgt tgcgcatgac
     2041 ctttcagttt gccgctcagt tccgggatca ccagaccaat ggcttttgcc gcccccgtag
     2101 tgtggggaat gatattttct gccgctgcgc gtgaagcacg taaatcttta ccacgcgggc
     2161 catccaccag tgactgggtg ccagtatagg catgaatggt cgtcatcgtg ccgacttcta
     2221 tcccgaaact gtcatgcaag gctttggcca tcggcgcaag gcagttagtg gtgcatgacg
     2281 ccacggaaac aatggtgtcg ttgccatcca gagtgtcgtc attaacgtta taaacgatgg
     2341 ttttcatttc accggcagga gcggaaatca acaccttctt cgcaccagca tcaagatgcg
     2401 cctgcgattt atcggcggag gtataaaagc cagtacattc gacaatgatt tctgcccctt
     2461 tcgctttcca cggaatattt ttagcctctt tttcggcgta aaccgcgata cttttcccat
     2521 caacgataag tgaatcttcc gtaaaatcaa cgctccaggg gaacggacca tagtttgaat
     2581 catgtttcag cagataggcg agaatttttg gggaagtaag atcattaata gcgacaacgt
     2641 ctatgttgct tttgacttca agtaaacgac gcaacaccag tcgaccgata cgaccaaaac
     2701 cgttaatacc aactttactc atggttttct cctgtcagga acgttcggat gaaaattgat
     2761 cctttccagg cttagaccag gattgtggga tgggcaatct ccattcttac agtgaaacgt
     2821 aacgtactga aaacgggtga acaatattta atgaaatttt gagaaaagcc cgttatgtta
     2881 acggaaaatt atgttaaagc aggaaatgtt atggaaaata aatactcaag gttacaaatc
     2941 agcattcact ggctggtctt tttgctggtt atcgcagcgt attgcgcaat ggagtttcgt
     3001 ggtttcttcc cacgtagcga tcggccgctc atcaacatga ttcatgtttc ctgtggcatc
     3061 tcaattctcg tgctgatggt ggttcgtctt ttgttaagac tgaaataccc aaccccgccg
     3121 attataccta agccgaaacc gatgatgacg ggactggcgc atttgggaca tttggtgatt
     3181 tatctcctgt ttattgcgct gccagtgatt ggtttggtga tgatgtataa ccggggcaac
     3241 ccgtggtttg cgtttggttt gacgatgcct tacgcttcag aggccaattt cgaacgggtt
     3301 gatagcttaa agtcgtggca tgagacgctg gcgaatctgg gatattttgt catcgggttg
     3361 cacgctgcgg ctgcactggc acaccactat ttctggaagg acaacacact tctacgcatg
     3421 atgccgcgta aacgttcctg aaggatattt aaagaaaacg cctgtactaa aaccgacccg
     3481 tggtacaggc gaagaatacg ggtctacatc ggaagcgcct attatattta tttgtatgac
     3541 aaataaaacc ataatccttg cccatacgtc catctggctt atttttaatc aattcacccg
     3601 atctttgatc tcatcaacgg tatcaaaata aaccagcgta taacgttcaa atcgcacata
     3661 agattcgata aaccatggag gttatatgaa aaaactggca cttattttgt ttatgggaac
     3721 gcttgtttcc ttttatgccg atgccgggcg caaaccctgt tctggttcga aaggggggat
     3781 ctcacactgt acggcaggcg gcaaatttgt ctgtaatgat ggttctatta gtgcatcgaa
     3841 aaaaacatgc actaactgaa gtgtaaaagg ggtgccatga gaaaatgaat ctgctagtca
     3901 gatgcgcggg gaaaatcccc gcgcttgccc ttacctggac gtgcaggcca tgagcgcagc
     3961 aacctcctta tcaccgtccc ggaaccgcag ttcgtagagc gtttgtcggg tcaggagtgt
     4021 gaatgccaga atcgtaatgc agataatgag cagacacacc accagagggt tgcgcttcat
     4081 agcctttctc cttgccggat ggcgggtaag aggctaacaa tctgaattgc ttggttcatt
     4141 cgttggcctc ggtttgtact gaccccaaaa agttggacag ttatacacga ggcatatagg
     4201 tctgattccg atattcattt ggagtcagac cttttaattt caggctaat
//
LOCUS       DAJTRC010000193         4211 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.197, whole genome shotgun sequence.
ACCESSION   DAJTRC010000193 DAJTRC010000000
VERSION     DAJTRC010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4211)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4211)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4211
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.197"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO357_003984"
     CDS             complement(<1..209)
                     /gene="tuf"
                     /locus_tag="OO357_003984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5888728.1"
                     /translation="MSKEKFERTKPHVNVGTIGHVDHGKTTLTAAITTVLAKTYGGAA
                     RAFDQIDNAPEEKARGITINTSHVE"
     gene            complement(324..399)
                     /locus_tag="OO357_003985"
     tRNA            complement(324..399)
                     /locus_tag="OO357_003985"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(364..366),aa:Thr,seq:ggt)
     gene            complement(406..480)
                     /locus_tag="OO357_003986"
     tRNA            complement(406..480)
                     /locus_tag="OO357_003986"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(445..447),aa:Gly,seq:tcc)
     gene            complement(597..681)
                     /locus_tag="OO357_003987"
     tRNA            complement(597..681)
                     /locus_tag="OO357_003987"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(645..647),aa:Tyr,seq:gta)
     gene            complement(690..765)
                     /locus_tag="OO357_003988"
     tRNA            complement(690..765)
                     /locus_tag="OO357_003988"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(730..732),aa:Thr,seq:tgt)
     gene            1127..2077
                     /gene="coaA"
                     /locus_tag="OO357_003989"
                     /gene_synonym="panK"
     CDS             1127..2077
                     /gene="coaA"
                     /locus_tag="OO357_003989"
                     /gene_synonym="panK"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HCR5888729.1"
                     /translation="MSIKEQTLMTPYLQFDRNQWAALRDSVPMTLSEDEIARLKGINE
                     DLSLEEVAEIYLPLSRLLNFYISSNLRRQAVLEQFLGTNGQRIPYIISIAGSVAVGKS
                     TTARVLQALLSRWPEHRRVELITTDGFLHPNQVLKERGLMKKKGFPESYDMHRLVKFV
                     SDLKSGVPNVTAPVYSHLIYDVIPDGDKTVVQPDILILEGLNVLQSGMDYPHDPHHVF
                     VSDFVDFSIYVDAPEDLLQTWYINRFLKFREGAFTDPDSYFHNYAKLTKEEAIKTAMT
                     LWKEINWLNLKQNILPTRERASLILTKSANHVVEEVRLRK"
     gene            complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO357_003990"
     CDS             complement(2106..3071)
                     /gene="birA"
                     /locus_tag="OO357_003990"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional biotin--[acetyl-CoA-carboxylase]
                     ligase/biotin operon repressor BirA"
                     /protein_id="HCR5888730.1"
                     /translation="MKDNTVPLKLIALLANGEFHSGEQLGETLGMSRAAINKHIQTLR
                     DWGVDVFTVPGKGYSLPEPIQLLNAEQILGQLDGGSVAVLPVIDSTNQFLLDRIGELK
                     SGDACVAEYQQAGRGRRGRKWFSPFGANLYLSMFWRLEQGPAAAIGLSLVIGIVMAEV
                     LRKLGADKVRVKWPNDLYLQDRKLAGILVELTGKTGDAAQIVIGAGINMAMRRVEESV
                     VNQGWITLQEAGINLDRNTLAAMLIRELRAALELFEQEGLAPYLSRWEKLDNFINRPV
                     KLIIGDKEIFGISRGIDKQGALLLEQDGIIKPWMGGEISLRSAEK"
     gene            complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO357_003991"
     CDS             complement(3068..4096)
                     /gene="murB"
                     /locus_tag="OO357_003991"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HCR5888731.1"
                     /translation="MNHSLKPWNTFGIDHNAQHIVCAEDEQQLLNAWQHATAEGQPVL
                     ILGEGSNVLFLEDYRGTVIINRIKGIEIHDEPDAWYLHVGAGENWHRLVKYTLQEGMP
                     GLENLALIPGCVGSSPIQNIGAYGVELQRVCAYVDCVELATGKQVRLTAKECRFGYRD
                     SIFKHEYQDRFAIVAVGLRLPKEWQPVLTYGDLTRLDPTTVTPQQVFNAVCHMRTTKL
                     PDPKVNGNAGSFFKNPVVSAETAKALLAQFPTAPNYPQAGGSVKLAAGWLIDQCQLKG
                     MQMGGAAVHRQQALVLINEDNAKSEDVVQLAHHVRQKVGEKFNVWLEPEVRFIGASGE
                     VSAVETIS"
BASE COUNT         1089 a         1047 c          937 g         1138 t
ORIGIN      
        1 tattcaacgt gagaagtgtt gatggtgata ccacgagctt tttcttccgg cgcgttatcg
       61 atctggtcga atgcgcgagc agcaccgccg taggttttag ccagtacggt agtgattgca
      121 gcggtcagcg ttgttttacc atggtcaacg tggccgatag taccgacgtt aacgtgcggt
      181 tttgtacgtt caaacttttc tttagacatc gattgtccct ctaagacacg gataaatcgg
      241 tgatatcacc acatcaacca ggcaacatgc ccgacttgtt gaatgcaata aacagaagga
      301 aaaaacaggg aggagaaaag aagtggtgct gataggcaga ttcgaactgc cgacctcacc
      361 cttaccaagg gtgcgctcta ccaactgagc tatatcagca catcttggag cgggcagcgg
      421 gaatcgaacc cgcatcatca gcttggaagg ctgaggtaat agccattata cgatgcccgc
      481 atcctagaac tcggctacct gattttcatt ctgcactgaa tatcgagaga agctctcttt
      541 attcgagccg gtaagtgaac ttatcgtctc gggctacgcc atcgcgtggc cgaaattggt
      601 ggtgggggaa ggattcgaac cttcgaagtc tgtgacggca gatttacagt ctgctccctt
      661 tggccgctcg ggaaccccac cggacttgat ggtgccgact accggaatcg aactggtgac
      721 ctactgatta caagtcagtt gctctaccta ctgagctaag tcggcatcaa gtagcgcgca
      781 ttctatggag acatgcgagt tcatgcaact aaaaaattgc ataatttgtt ttattggtca
      841 cattttatgc tacacgatga agaaacagcc gaacggaggg cagattagca cactttttca
      901 acatcattgt gctcaacaat gcgctcctgc taaaccataa ttctttttat cagatggaat
      961 atctgtcaca ttgcttttca acgatagctt cctggcagag attttttctt attattcctc
     1021 cccatctggt gttaccctcc tgcccattaa cccattcaac agaactgtga cgcgccatgg
     1081 caaatatcgc tttgccgata gagctatgac cgccagaaac atgcttatga gtataaaaga
     1141 gcaaacgtta atgacgcctt acctacagtt tgaccgcaac cagtgggcag ctctgcgtga
     1201 ttccgtacct atgacgttat cggaagatga gatcgcccgt ctcaaaggta ttaatgaaga
     1261 tctctcgtta gaagaagttg ccgagatcta tttacctttg tcacgtttgc tgaacttcta
     1321 tataagctcg aatctgcgcc gtcaggcagt tctggaacag tttcttggta ccaacgggca
     1381 acgcattcct tacattatca gtattgctgg cagtgtcgcg gtggggaaaa gtacaaccgc
     1441 ccgtgtattg caggcgctat taagccgttg gccggaacat cgtcgtgttg aactgatcac
     1501 tacagatggc ttccttcacc ctaatcaggt tctgaaagaa cgtggtctga tgaagaagaa
     1561 aggcttcccg gaatcgtatg atatgcatcg cctggtgaag tttgtttccg atctcaaatc
     1621 cggcgtgcca aacgttacag cccctgttta ctcgcatctt atttatgatg tgatcccgga
     1681 tggagataaa actgttgttc agcctgatat tttaattctt gaagggttaa atgtcttaca
     1741 gagcgggatg gattatccac acgatccaca tcatgtattt gtttccgatt ttgtcgattt
     1801 ttcgatatat gttgatgcac cggaagactt acttcagacg tggtatatca accgttttct
     1861 gaaattccgc gaaggggctt ttacagaccc ggattcctat tttcataact acgcgaaatt
     1921 aactaaagaa gaagcgatta agactgccat gacattgtgg aaagagatca actggctgaa
     1981 cttaaagcaa aatattctac ctactcgtga gcgcgccagt ttaatcctga cgaaaagtgc
     2041 taatcatgtg gtagaagagg tcagactacg caaataattt gcaggggagc gaatactccc
     2101 ctttcttatt tttctgcact acgcagggat atttcaccgc ccatccaggg ttttattatt
     2161 ccatcctgct caagtaataa agccccctgt ttgtctattc cgcgtgaaat gccaaatatt
     2221 tctttatcac caatgataag tttcactggg cgattaataa aattatccag cttttcccag
     2281 cgcgacagat aaggtgccaa tccttcttgt tcgaagagtt ccaacgcagc acgtaattca
     2341 cgtattagca tggccgccaa cgtattacga tcaagattga tccccgcttc ctgcagcgtg
     2401 atccacccct gattaacgac actctcttca acacggcgca ttgccatgtt gatcccggct
     2461 ccaatgacta tttgcgccgc atcgccagtt ttgccagtca gctccacaag aatgcctgcc
     2521 agcttgcgat cctgcagata gaggtcatta ggccatttga cacgaacttt atctgctccc
     2581 agctttcgta atacttccgc catcacgata ccgataacca gacttaaacc aatcgccgcc
     2641 gccgggcctt gttccagacg ccagaacatc gacaaatata agtttgcgcc aaaaggcgaa
     2701 aaccatttcc gcccccggcg accacggcca gcctgctggt attctgcaac acaggcatcg
     2761 cccgatttaa gctctccgat acgatcaaga aggaactgat tcgtggagtc aataactggc
     2821 agcacggcta cactaccgcc atccagctga cccaatatct gttcagcatt aagtaactgg
     2881 atgggctcag gcaggctgta tcctttaccc ggaacggtaa agacatcaac gccccagtca
     2941 cgcagtgtct gaatgtgttt attaatagcc gcccggctca ttcccagcgt ttcacccaac
     3001 tgctcgccag agtgaaattc accgttcgct aacagggcaa tcaatttcag tggcacggtg
     3061 ttatccttca tgaaattgtc tccactgcgc tcacttcacc tgatgcacca ataaagcgga
     3121 cttcaggctc aagccagaca ttaaattttt cacccacttt ttgtcttaca tggtgtgcca
     3181 gttgcaccac atcttcgctt tttgcattgt cttcattaat gagaactaac gcctgttgac
     3241 ggtgcaccgc agccccaccc atttgcatcc cttttagctg gcactgatca ataagccaac
     3301 ctgctgccag ttttactgaa ccacccgcct gggggtaatt tggtgctgtt ggaaattgcg
     3361 ccagtaatgc tttagccgtt tcggcagata caacagggtt tttgaagaaa ctaccggcat
     3421 tgccattcac ttttggatca gggagtttgg tggtgcgcat atgacacacc gcattaaata
     3481 cttgctgtgg cgttactgtt gtaggatcca gacgagttaa gtcaccatac gttagtacag
     3541 gttgccactc ttttggcaga cgcagaccta cggctacaat ggcgaagcga tcctggtatt
     3601 catgtttaaa aatactgtcg cgatagccaa aacggcactc tttggcagtt aagcgcactt
     3661 gcttgcctgt cgccagttca acacaatcaa cataagcgca aactcgctgt aattctacgc
     3721 cataagcacc aatattctgg ataggtgatg agccgacaca accaggaatt aatgccagat
     3781 tttccagacc aggcatacct tcctgcaaag tgtattttac caggcgatgc cagttttctc
     3841 cggctcctac atgtaaatac cacgcatcag gttcatcatg aatttcgata cctttgatcc
     3901 ggttgatgat caccgtgccg cgatagtctt ccagaaaaag tacattactt ccttcaccca
     3961 gaataagaac gggttgtcct tctgcggttg catgctgcca ggcattgagt aattgttgtt
     4021 cgtcttcggc acatacaatg tgctgagcat tatgatcaat gccaaatgtg ttccagggtt
     4081 ttaaggagtg gttcatagct gctttcctga tgcaaaaacg aggctagttt accgtatctg
     4141 tggggggatg gcttgtagat atgacgacag gaagagtttg tagaaacgca aaaaggcccg
     4201 actgggcttt c
//
LOCUS       DAJTRC010000194         4198 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.198, whole genome shotgun sequence.
ACCESSION   DAJTRC010000194 DAJTRC010000000
VERSION     DAJTRC010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.198"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            84..752
                     /locus_tag="OO357_003992"
     CDS             84..752
                     /locus_tag="OO357_003992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888732.1"
                     /translation="MSEQIKQDIDLIEILFHLKKKIRVILFIMAICMAMVLLFLYINK
                     DNTKVIYSLKINQTTPGILVSCDSNNNFACQTTMTEDVIQRITTFFQTSPDIKNREIR
                     LEWSGDKRDLPTAEEEISRVQASIIKWYASEYHNGRQVLDEIQTPSAINSELYTKMIY
                     LTRNWSLYPNGDGCVTISSPEIKNKYPAAICLALGFFLSIVISVMFCLVKKMVDEYQQ
                     NSGQ"
     gene            complement(995..1690)
                     /locus_tag="OO357_003993"
     CDS             complement(995..1690)
                     /locus_tag="OO357_003993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein C"
                     /protein_id="HCR5888733.1"
                     /translation="MDNRSEFLNNVAQALGRPLRLEPQAEDAPLNNYANERLTQLNQQ
                     QRCDAFIQFASDVMLTRCELTSEAKAAEAAIRLCKELGDQSVVISGDTRLEELGISER
                     LQQECNAVVWDPAKGAENISQAEQAKVGVVYAEYGLTESGGVVLFSAAERGRSLSLLP
                     ESSLFILRKSTILPRVAQLAEKLHQKAQAGERMPSCINIISGPSSTADIELIKVVGVH
                     GPVKAVYLIIEDC"
     gene            complement(1683..3110)
                     /locus_tag="OO357_003994"
     CDS             complement(1683..3110)
                     /locus_tag="OO357_003994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster-binding protein"
                     /protein_id="HCR5888734.1"
                     /translation="MSIKTSNTDFKTRIRQQIEDPIMRKAVASAQQRIGANRQKMVDE
                     LGHWEEWRDRAAQIRDHVLSNLDAYLYQLSEKVTQNGGHVYFAKTKEDATRYILQVAQ
                     RKNARKVVKSKSMVTEEIGVNHVLQDAGIQVIETDLGEYILQLDQDPPSHVVVPAIHK
                     DRHQIRRVLHERLGYEGPETPEAMTLFIRQKIREDFLSAEIGITGCNFAVAETGSVCL
                     VTNEGNARMCTTLPKTHIAVMGMERIAPTFAEVDVLITMLARSAVGARLTGYNTWLTG
                     PREAGHVDGPEEFHLVIVDNGRSEVLASEFRDVLRCIRCGACMNTCPAYRHIGGHGYG
                     SIYPGPIGAVISPLLGGYKDFKDLPYACSLCTACDSVCPVRIPLSKLILRHRRVMAEK
                     GITAKAEQRAIKMFAYANSHPGLWKVGMMAGAHAASWFINGGKTPLKFGAISDWMEAR
                     DLPEADGESFRSWFKKHQAQEKKNG"
     gene            complement(3121..3840)
                     /locus_tag="OO357_003995"
     CDS             complement(3121..3840)
                     /locus_tag="OO357_003995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HCR5888735.1"
                     /translation="MNVNFFVTCIGDALKSRMARDSVLLLEKLGCRVNFPEKQGCCGQ
                     PAINSGYIKEAIPGMKNLIAALEDNDDPIISPAGSCTYAIKSYPTYLADEPEWASRAE
                     KVAARMQDLTSFIVNKLGVVDVGASLQGRAVYHPSCSLARKLGVKDEPLTLLKNVRGL
                     ELLTFAEQDTCCGFGGTFSVKMAEISGEMVKEKVVHLMEVRPEYLIGADVSCLLNISG
                     RLQREGQKVKVMHIAEVLMSR"
BASE COUNT         1119 a         1085 c          869 g         1125 t
ORIGIN      
        1 ttgctgatat ttaacccgaa cattcctgat acagactatt aatacaacat tatctgcgac
       61 agattttatt ttctggatat attatgagtg aacaaatcaa acaggatatc gatctgattg
      121 agattttatt tcatctgaag aaaaagattc gtgttatcct ttttattatg gctatctgta
      181 tggcaatggt gctgttgttt ctgtatatca ataaagacaa tacaaaagtg atttacagcc
      241 ttaaaataaa ccagacaacg ccaggtatac ttgttagctg tgatagcaat aataattttg
      301 cctgtcagac cacaatgact gaagatgtta ttcagcgaat tactacattt tttcagacca
      361 gcccagatat caagaacaga gaaataaggc tggaatggtc aggagataag agagatttac
      421 caactgctga agaggaaata tctcgcgtgc aggcgtctat tatcaaatgg tatgcgtcag
      481 aataccataa tggcaggcaa gttctcgatg agatacaaac gccttcagca attaacagtg
      541 agctttatac aaaaatgata tacctgacca ggaactggtc attgtatccg aacggtgatg
      601 gctgtgtaac tataagctca ccagaaataa aaaataaata ccctgctgcc atttgcctgg
      661 ctctgggatt ttttctaagc attgtaattt ctgtaatgtt ttgccttgtc aaaaaaatgg
      721 tagatgaata ccaacaaaac tctgggcagt aataaacagc attctgatag agagtgcctg
      781 tataaagctg aagtgacaat gaccgggaag ggaacccgtt ttcaggaatg gatgcctgtc
      841 tttttcactg taaatatcct ggcattacta atgcctggat atttacagaa gagcagcata
      901 accatgacgg aacggggtta agtcaggttg tccttaacgc tattttcaac ctcgtatgcc
      961 ttcttcaggt ttatgttcag atctcatatc tctctcagca atcctcaata atcagataca
     1021 ccgctttcac cggaccatga accccgacga ctttgataag ctcaatatcc gccgttgagc
     1081 tgggaccact gatgatgtta atacatgacg gcattcgttc accggcctgc gctttctgat
     1141 gcaatttttc tgcgagttgc gctacacgcg gcaggattgt gcttttacgc aggataaaaa
     1201 gagaagattc cgggagcagg ctcaatgaac gcccgcgctc ggcggcggaa aaaagaacca
     1261 cgcctcccga ttcggttaaa ccatattcag catacacgac acccacttta gcctgctctg
     1321 cctgcgagat attctcggca cctttcgccg gatcccaaac aacggcattg cattcctgct
     1381 gcaaacgttc actaatcccc aattcctcca gcctcgtgtc accgctaatc acgaccgact
     1441 gatctcccag ctctttacac agacgtattg cagcttctgc cgccttcgcc tcgctggtca
     1501 gctcacagcg cgtcaacata acatcgctgg caaactgaat aaacgcgtca cagcgctgct
     1561 gttggttaag ttgggtaagc cgctcgttag catagttgtt aagcggcgca tcttctgctt
     1621 gcggttcaag tcgcagcggg cgacccagtg cctgagcaac gttattcaaa aattcgctcc
     1681 gattatccat tctttttctc ctgcgcctga tgtttcttaa accaactacg gaaactctct
     1741 ccgtcagctt caggaagatc gcgtgcttcc atccagtcgc taatcgcgcc aaatttgagt
     1801 ggtgttttgc cgccattgat aaaccagctt gccgcatgag cgccggccat catcccgact
     1861 ttccacaatc ctggatgact attggcataa gcgaacattt ttatcgcccg ttgctctgct
     1921 tttgcggtga tccctttttc agccatcacc cgacgatgac gcaaaatcag ttttgacagc
     1981 ggaatacgca ccggacacac gctgtcacaa gcagtgcata aagagcaggc gtagggtaaa
     2041 tctttaaaat ctttatagcc gccaagtagc ggagaaatca ccgcaccaat tggccctgga
     2101 taaatagagc catatccatg accgccaata tggcgatatg ccggacaagt attcatacaa
     2161 gccccgcagc gaatacagcg cagcacatcc cgaaattcag aggccagcac ctcagaacgc
     2221 ccgttatcga caataaccag atgaaactct tcaggaccat caacgtgccc cgcttcgcgc
     2281 ggccctgtca gccaggtgtt gtatcccgtc aaacgtgcac caacggcact gcgcgccagc
     2341 atggtgatca atacatctac ctcggcaaac gtgggggcaa tacgctccat tcccatcact
     2401 gcaatatgcg ttttaggcag cgtggtacac attcgcgcat taccttcatt ggtcaccagg
     2461 cataccgaac cggtctctgc caccgcgaaa ttacagccgg taatacctat ttcagcactg
     2521 aggaaatctt cacggatttt ttgccggatg aataaggtca tcgcttcagg cgtttccggc
     2581 ccctcatagc ccagacgttc gtgtagcact cgacggatct gatggcgatc tttatgaatt
     2641 gccgggacca caacatgaga tggcggatct tgatccagct ggagaatata ttcacccaga
     2701 tcggtttcaa tcacctgaat gccagcatcc tgcaacacat gattgacacc aatctcttcg
     2761 gtcaccatcg atttagattt caccaccttc cgggcatttt tgcgttgggc aacctgtaaa
     2821 atgtagcggg tagcgtcttc tttggttttt gcaaaataga cgtgaccgcc gttttgcgtc
     2881 actttttctg agagctggta cagataagcg tcgagattac tcagaacatg atcacgtatc
     2941 tgggcggccc gatcgcgcca ctcctcccag tgccccaatt catcgaccat tttttgccga
     3001 tttgccccaa tacgctgctg cgcgcttgcc accgctttgc gcatgatcgg atcttcaatt
     3061 tgctgacgga tgcgtgtctt aaaatctgta ttactggttt tgatcgacat ctttatatcc
     3121 tcagcggctc atcaacactt cagcaatatg catcactttg actttctgcc cttcccgttg
     3181 taatcgccca ctgatgttca gcaggcaact cacgtcagca ccaattaaat actcagggcg
     3241 gacttccatc aggtgcacaa ccttttcttt caccatctcg ccggatattt cggccatttt
     3301 gaccgagaac gtgccgccaa atccgcagca ggtatcctgt tcagcaaagg tcaacagctc
     3361 cagtccacgc acatttttca gcagcgtaag tggctcgtcc ttcactccca gcttacgggc
     3421 caggctacaa gatgggtgat acacagctct cccttgcaaa ctggcaccta catcgactac
     3481 ccctaattta ttaacaataa aagaggtgag atcctgcata cgcgcggcaa ccttttcggc
     3541 acgtgatgcc cattcaggtt catccgccag atacgtcggg taacttttta tggcataggt
     3601 gcaagagcca gccggtgaaa taatgggatc gtcgttatcc tccagtgcgg cgatcagatt
     3661 tttcatccct ggaatcgctt ctttgatata accgctattg atcgcaggct gaccgcagca
     3721 tccctgtttc tccgggaaat ttacgcgaca gccgagtttt tccagtagca gcacggagtc
     3781 tcgtgccatt cttgatttca gggcgtcacc aatacaggtg acaaagaaat tgacattcac
     3841 aactaatact ccattacttc atgcccattt atgcccgatg tttccccccc accgggggat
     3901 caatcatcaa taaatatggc gttctttttt aattggctag caacatacca cccctcattt
     3961 gtatgacaaa tgataatctt tcatccaaac ttcaaatgca ataactaatt ttatttcttt
     4021 cggcatcgct cacattttat ggggaaatcg acgatcattt ttggcataag aaatccattt
     4081 aaaaatggct acatccctta tacctattca gagctactta ttcaggggtt attgagatat
     4141 acactgatgg tgtacaaaaa aatcaccgac aataatcacg tatcaagtaa tagtcgga
//
LOCUS       DAJTRC010000195         4172 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.199, whole genome shotgun sequence.
ACCESSION   DAJTRC010000195 DAJTRC010000000
VERSION     DAJTRC010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4172)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4172)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4172
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.199"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..176)
                     /locus_tag="OO357_003996"
     CDS             complement(<1..176)
                     /locus_tag="OO357_003996"
                     /inference="COORDINATES: protein motif:HMM:NF017243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WGR domain-containing protein"
                     /protein_id="HCR5888736.1"
                     /translation="MRHFIYQDEKSHKFWTVEQQGNELHISWGKVGTKGQSQIKSFSD
                     AAAAEKAELKLIAE"
     gene            complement(317..2350)
                     /gene="metG"
                     /locus_tag="OO357_003997"
     CDS             complement(317..2350)
                     /gene="metG"
                     /locus_tag="OO357_003997"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708001.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HCR5888737.1"
                     /translation="MTQVAKKILVTCALPYANGSIHLGHMLEHIQADVWVRYQRMRGH
                     EVNFICADDAHGTPIMLKAQQLGITPEQMIGEMSQEHQTDFAGFNISYDNYHSTHSEE
                     NRQLSELIYSRLKENGFIKNRTISQLYDPEKGMFLPDRFVKGTCPKCKSPDQYGDNCE
                     VCGATYSPTELIEPKSVVSGATPVMRDSEHFFFDLPSFSEMLQAWTRSGALQEQVANK
                     MQEWFESGLQQWDISRDAPYFGFEIPNAPGKYFYVWLDAPIGYMGSFKNLCDKRGDSV
                     SFDEYWKKDSTAELYHFIGKDIVYFHSLFWPAMLEGSNFRKPTNLFVHGYVTVNGAKM
                     SKSRGTFIKASTWLNHFDADSLRYYYTAKLSSRIDDIDLNLEDFVQRVNADIVNKVVN
                     LASRNAGFINKRFDGVLASELADPELYKTFTDAAEVIGEAWESREFGKAVREIMALAD
                     LANRYVDEQAPWVVAKQEGRDADLQAICSMGINLFRVLMTYLKPVLPKLTERAEAFLN
                     TELTWDGIQQPLLGHKVNPFKALYNRIDMKQVEALVEASKEEVKATTAPVTGPLADDP
                     IQETITFDDFAKVDLRVALIENAEFVEGSDKLLRLTLDLGGEKRNVFSGIRSAYPDPQ
                     ALIGRHTIMVANLAPRKMRFGISEGMVMAAGPGGKDIFLLSPDAGAKPGHQVK"
     gene            2482..3591
                     /gene="apbC"
                     /locus_tag="OO357_003998"
     CDS             2482..3591
                     /gene="apbC"
                     /locus_tag="OO357_003998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster carrier protein ApbC"
                     /protein_id="HCR5888738.1"
                     /translation="MNEQSQAKSPEALRAMVAGTLANFQHPTLKHNLTTLKALHHVAW
                     MDDTLHVELVMPFVWHSAFEELKEQCSAELLRITGAKAIDWKLSHNIATLKRVKNQPG
                     INGVKNIIAVSSGKGGVGKSSTAVNLALALAAEGAKVGILDADIYGPSIPTMLGAENQ
                     RPTSPDGTHMAPIMSHGLATNSIGYLVTDDNAMVWRGPMAGKALMQMLQETLWPDLDY
                     LVLDMPPGTGDIQLTLAQNIPVTGAVVVTTPQDIALIDAKKGIVMFEKVEVPVLGIVE
                     NMSVHICSNCGHHEPIFGTGGAEKLAEKYHTQLLGQMPLHISLREDLDKGTPTVISRP
                     ESEFTAIYRQLADRVAAQLYWQGEVIPGEISFRAV"
     gene            3853..4134
                     /locus_tag="OO357_003999"
     CDS             3853..4134
                     /locus_tag="OO357_003999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehE family protein"
                     /protein_id="HCR5888739.1"
                     /translation="MNKYWLSGIIFLAYGLASPAVSSETATLTINGRISPPTCSMAMV
                     NSQPQQHCGQLTYNVDTRHQVSSPVKGVTTEVVVAGSDSKRRIVLNRYD"
BASE COUNT         1015 a         1038 c         1062 g         1057 t
ORIGIN      
        1 ttctccgcaa tcagcttcag ttccgctttt tccgccgccg cagcatctga aaaacttttt
       61 atctgacttt gccctttggt gccaactttt ccccaactga tatgcaactc gtttccctgt
      121 tgctccaccg tccagaattt atgtgatttt tcgtcctgat agataaagtg tctcattctg
      181 catccctata attttatcca gttgaatgca gatgctacca gtatttatac gggttagaga
      241 gagacaaaat cgcaagaaaa caactttagg aatttataaa gaaaaggcgc tgtcgatgca
      301 gcgccttgaa gggggattat ttcacctgat gacccggttt agcaccggca tccgggctta
      361 gcaggaaaat atctttcccg ccaggaccgg cagccatcac catgccttca gagataccga
      421 agcgcatttt acgtggtgcc aggttagcca ccataatggt gtgacgacca atcagtgcct
      481 gcggatccgg gtaagcagaa cgaataccgg agaagacatt gcgtttttca ccgccgagat
      541 ccagcgtcag gcgcagcagt ttgtcagaac cttcaacaaa ctctgcgttt tcgatcagcg
      601 ccacgcgcag gtcaactttg gcgaagtcgt caaaggtgat ggtttcctga atcggatcat
      661 ctgccagtgg gccagtaact ggcgcggtag tggctttcac ttcttcttta gaggcttcca
      721 ccagagcttc aacctgtttc atatcgatgc ggttatacag cgccttgaac ggattcactt
      781 tgtggcccag cagcggttgc tggataccat cccaggtcag ttccgtattg aggaaggctt
      841 ctgcacgctc ggtcagtttc ggcagtacag gcttcaggta agtcatcagt acgcggaaca
      901 ggttgatgcc cattgagcaa atcgcctgca ggtcagcatc gcgaccttcc tgttttgcga
      961 ctacccacgg agcctgctcg tcgacatagc ggttagccag gtcagccagt gccatgattt
     1021 cgcgcactgc tttaccaaat tcacggcttt cccacgcttc accaatcact tcagcggcat
     1081 cggtaaaggt tttgtacagc tcagggtcag ccagctcgct tgccagcaca ccgtcaaaac
     1141 gcttgttgat aaagcccgca ttacgggagg ccaggttaac cactttgttc acgatatcgg
     1201 cattcacacg ctgaacgaaa tcttccaggt tgagatcgat atcatcaatg cgcgaagaga
     1261 gtttcgcagt gtagtagtaa cgcaggctgt cagcgtcaaa atgattcagc caggtgctgg
     1321 ctttaataaa ggtgccgcga gacttggaca tcttcgcgcc gttcaccgtc acatagccat
     1381 gaacaaacag gttggtcggc ttgcggaagt tgctgccttc cagcatggca ggccagaaca
     1441 ggctgtggaa gtaaacgata tctttaccga tgaagtggta cagctcggcg gtggagtctt
     1501 tcttccagta ttcatcgaag cttacgctgt cgccgcgctt gtcgcacaga ttcttgaaag
     1561 aacccatgta gccaatcggt gcgtccagcc agacgtagaa atatttgccc ggtgcgttcg
     1621 gaatttcaaa accaaagtat ggcgcgtcgc gggagatatc ccactgttgc aggccggatt
     1681 caaaccactc ctgcattttg tttgccacct gctcctgcaa cgcaccgctg cgggtccatg
     1741 cctgcagcat ttcgctgaaa gagggcagat caaagaagaa gtgttcagaa tcacgcatca
     1801 ctggtgtagc accagaaacc accgatttcg gctcgatcaa ttcagtcggg ctgtaggtcg
     1861 cgccgcagac ttcgcagtta tcgccgtatt gatccgggga tttacatttc gggcaggtgc
     1921 ctttcacaaa acggtccggc aggaacatgc ctttttccgg atcgtacagc tgagagatgg
     1981 tgcggttttt aataaaaccg ttttctttca ggcgagagta gataagttct gacaactggc
     2041 ggttctcttc gctgtgcgtc gagtgatagt tgtcatagct gatgttaaag cctgcgaaat
     2101 cagtctgatg ctcctgactc atttcgccaa tcatctgctc cggggtgata ccgagctgct
     2161 gagctttcag catgatcggt gtaccgtggg catcgtcggc gcagatgaag ttgacctcgt
     2221 ggccgcgcat tcgctggtaa cggacccaga catcagcctg gatgtgctcc agcatatggc
     2281 cgaggtggat tgagccgtta gcgtacggca gtgcgcacgt caccagaatt ttcttcgcga
     2341 cttgagtcat agtaggcatt acttcttaat agtgaaaagg ggttagatag taccaaatgg
     2401 gaaaatgtta agtaagtact tctctatatt cttcaggtaa actatcagct gatggtttat
     2461 ctatacaaaa aaggagtcgg gatgaacgaa caatcccagg ccaaatcacc agaagccttg
     2521 cgcgcaatgg tcgccgggac actggccaat tttcagcacc caaccctgaa gcataacctc
     2581 accacgctga aagcgttgca ccatgtcgcc tggatggacg acacgttgca tgtggaactg
     2641 gttatgccgt ttgtctggca tagcgcgttc gaagagttaa aagagcaatg tagcgccgaa
     2701 ctgctgcgca tcaccggggc gaaagctatc gactggaaac tgtcgcataa catagccact
     2761 ctgaaacgcg tgaagaatca gccgggaatt aacggtgtga agaacattat cgcggtgagt
     2821 tccggtaaag gcggcgtggg taaatcgtcc acggcggtaa acctggcgct ggcactggcg
     2881 gcggaagggg cgaaagtcgg cattctggac gccgatatct acggcccatc aattcccacc
     2941 atgctgggcg cagaaaatca gcgtccaact tcgccggacg gtacccacat ggcaccgatt
     3001 atgtctcatg gcctggcaac caattcaatt ggttatctgg tcactgacga caatgcgatg
     3061 gtgtggcgtg gaccgatggc gggcaaggcg ttaatgcaga tgttgcagga aaccttgtgg
     3121 ccggatctcg actatctggt gcttgatatg ccgccaggca ccggtgacat tcaattgacg
     3181 ctggcgcaaa acattccagt aactggtgca gtcgtggtca ccaccccgca ggacatcgcg
     3241 ttgatcgatg cgaaaaaagg cattgtgatg ttcgagaaag tcgaggttcc ggtgctcggt
     3301 attgtcgaga acatgagtgt gcatatttgc agtaactgtg gtcaccatga accgattttc
     3361 ggtaccggtg gtgcagagaa actggctgaa aaataccata cacagctgct ggggcagatg
     3421 ccgttacaca tctccttacg tgaggatctc gataaaggca cgccaacggt tatcagccgt
     3481 ccggagagcg aatttaccgc tatctaccgc cagctggctg accgcgttgc agctcagctc
     3541 tactggcagg gtgaagtcat tcctggcgag atttccttcc gcgcggtcta acgcctatta
     3601 accattctgg ttattcttca tacaccgcag agatgttata ttgatgcggt gtatttgttt
     3661 ttgccgcatt aatgaacact tatttaatac tgcgaatatt ctgcggacct tcgtccgaaa
     3721 ctctacaaag tcgagtgatc gtacaggcaa tgcttcatta tcatccttcg tgctgtgtag
     3781 tggatgtaca gctatagttt gttatgcact cattctatcc aggcataacc tttactacag
     3841 ggataacgtt caatgaataa gtattggctg tcaggaatta tttttcttgc ctatggattg
     3901 gcttctcccg cagtctcttc agagactgcc acattaacga ttaatggcag gatcagtccc
     3961 ccaacatgca gtatggctat ggttaatagt caaccacaac agcattgtgg tcagttaacg
     4021 tataatgttg acacccgtca tcaggtttct tcgcccgtca aaggtgttac gacagaagtg
     4081 gttgttgcag gtagcgatag caaacgtcga atcgtgttaa atcgctacga ttaatttggc
     4141 aacctgattt taattgcatt tagaaagacg aa
//
LOCUS       DAJTRC010000196         4085 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.200, whole genome shotgun sequence.
ACCESSION   DAJTRC010000196 DAJTRC010000000
VERSION     DAJTRC010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4085)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.200"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            126..1214
                     /locus_tag="OO357_004000"
     CDS             126..1214
                     /locus_tag="OO357_004000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005077865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HCR5888740.1"
                     /translation="MSTALVLTQSRVGLLGLFCVIVLAALADRPRWRGRPLSGLLRLI
                     FVASLLCWCVGISNYAMPGETTADFTHSSSNDQRIRILKGAISLIQQRPLTGWGLGSF
                     EATFPQALEDVGVTNIESDTATHPHNELLFVTAEGGIVALFGFLLLSGVWLWPCICRL
                     KMTNNNAHHQCEKWFIALMGLPLVIHMMTEYPLYTSVPHLMLLLLLFRIGMPENVMNQ
                     VRIPKTVRLGILPVIVLIMTAALVILVAGFDAQRELTRAEADMNEGFIPRLPEHSWKY
                     LTQAERLERDQHLLIANKPGFVRDTESMSLFTVWGNKWLAIHNDADVSAAMIFIAHRR
                     GDYLTAEKIRKRAARVFVNDERFTRGGN"
     gene            1214..1948
                     /locus_tag="OO357_004001"
     CDS             1214..1948
                     /locus_tag="OO357_004001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000245840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5888741.1"
                     /translation="MVNDLTYKKLESTNINCNILSPENTTWTNVTVIGLDLFAICGVC
                     SLLKRVGGVKLQVFTSLPTDMNVLTDSSIVIWVKMRHDGLPELAGHIVKVCRRYRLMR
                     QLVISDAIPEGMTNDIGPLSRVWLTHGSAKVDILLSSLRKVLNAHCLPGPLFIKRLGR
                     VQWRVLLLRAKGISTRKIAEICGIGLKTVSAHESAIRERLGTRNKSEYVWLVRNAQLI
                     QQAIPALGRDASRYERCNNMRTKQKV"
     gene            2415..3176
                     /locus_tag="OO357_004002"
     CDS             2415..3176
                     /locus_tag="OO357_004002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005104236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5888742.1"
                     /translation="MKNFNVLTKVVFCLSFLSSAFPTYAADDVKVTQNTSVFKLKLSA
                     TRLIYEQGSDGAAITVTNPQDWPMLVQSKVFEEDKVTAAPFIVTPPLFRLNPGQQNKM
                     WVVQTAPALQDREKLHWLCATGITPKKSDVWAEKDKEKIAENVNLNIQVNVHTCIKLI
                     TRPSGIEGKMVNASEKLSWKKQGDKIVAVNQSPFYINLSSLQVGGSKIEKLDYIAPFS
                     QHEFVMPKTSGQDIKWSVITDEGGESRLFISHLQS"
     gene            3220..>4085
                     /locus_tag="OO357_004003"
     CDS             3220..>4085
                     /locus_tag="OO357_004003"
                     /inference="COORDINATES: protein
                     motif:HMM:NF012786.1,HMM:NF025320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5888743.1"
                     /translation="MFYRLSLILIMALLAGQLSAQEWSFDSSQLEGNVSADTVAMFNQ
                     GEQLPGNYRVEIYLNGEKVDVGEFPFHRPESPEEKELVPCLTVDDLIHYGIKIDKSSS
                     DTDNKKNQCFKWNSIEGLKVNYDFDSQRVQITVPQLYLQDKKSSLAPVSLWNEGVAAF
                     RMVYQTNIDISKQNDNQSTTRNSRYGRFTPGFNLGAWRFRSSVTWSKELGQSERWQRG
                     YMWFERGINAIKSRLTLGESYTSSEVFDSIPFRGGMLATDDAMTPPEDSYYTPVVHGI
                     AQSEAQVIIKQNG"
BASE COUNT         1131 a          779 c          998 g         1177 t
ORIGIN      
        1 gcattttcca gcagcgaaat gttcttgcga gctggctggc aacaggttgc ggcgtagcgt
       61 tgtatctggc tttaacggcg cgtactcgtt ctctgacgtt gatatgtttg ttgtcgttat
      121 accctctgtc tacagcttta gtccttacac aaagccgtgt cggccttctt ggtttatttt
      181 gcgtcatcgt gctggccgct ctcgcggaca gaccccgctg gcgcggacgt ccccttagcg
      241 gtctgttgcg cttgatcttc gtcgcctcct tgctgtgctg gtgtgtcggt atctcaaact
      301 atgccatgcc aggggagaca acggctgact ttacgcattc atcatcgaat gaccagcgta
      361 tacgtattct gaaaggtgcc ataagtctta ttcaacagcg ccccttaacc ggttgggggt
      421 taggcagctt tgaagcgacc ttcccgcaag ccttagagga tgtgggcgtg acaaacatag
      481 agagtgatac ggctacgcat ccccataacg aactgctgtt cgtaacggct gaaggtggca
      541 tcgtcgcctt gtttggtttt ttgctgctat caggcgtttg gctgtggccg tgtatatgcc
      601 gcctgaaaat gacaaacaat aacgcgcacc atcaatgtga aaagtggttt attgccttaa
      661 tggggttgcc tttagtcatt catatgatga ccgaatatcc tctctatacg tctgttcccc
      721 atttgatgct gttgctgctc cttttccgca tcggaatgcc ggaaaacgtg atgaatcagg
      781 tacgtatacc taaaacagtc cggttgggta ttctgcctgt cattgtgctc attatgaccg
      841 ccgcgctggt catcctggta gcgggatttg atgcacagcg ggaactgacc agagctgaag
      901 ctgatatgaa cgaaggattt atccctcgcc ttccggaaca tagctggaaa tacctgacgc
      961 aggcagaacg tcttgagcgc gatcagcatc tgcttattgc gaataaaccg gggttcgttc
     1021 gcgatacgga aagcatgtct cttttcactg tgtggggaaa taaatggttg gcaatccaca
     1081 atgatgcaga cgtaagcgct gcaatgatat tcattgcgca caggcgcggt gattatctga
     1141 cagctgaaaa aataagaaaa cgcgcagctc gggtatttgt aaatgacgaa cgttttactc
     1201 gcgggggaaa ctaatggtta acgatctgac gtataagaaa ttagaaagca caaacatcaa
     1261 ctgtaatatt ctctcgccag aaaataccac atggacaaat gtcacggtga ttggcctgga
     1321 tctttttgcc atttgcggcg tgtgcagttt gctcaagcgt gttgggggag tgaaacttca
     1381 ggttttcact tccttaccca ctgatatgaa tgttctgact gacagtagca tcgtgatatg
     1441 ggtaaaaatg cgtcacgatg gtttgcctga attagccgga catattgtga aggtttgtcg
     1501 tcgataccgt ctgatgcggc aactcgttat tagcgatgct attcctgaag gaatgacgaa
     1561 tgatatcggg cctctttccc gagtatggct tactcatggg agtgcaaaag tggatatttt
     1621 attatcttca ttacggaagg tgttaaatgc tcattgtttg cctggtcctt tgtttataaa
     1681 acgcctggga agagtgcaat ggagggttct tttactccgc gcgaagggga ttagcacacg
     1741 taaaattgca gaaatctgtg gtattggcct caagaccgtt agcgcccatg agtccgcaat
     1801 tcgagaacga ttaggcacca ggaataaaag tgagtacgtc tggttagtac gtaatgctca
     1861 acttatacaa caggcgatac ccgcgttggg aagagatgcg tcaaggtatg aaagatgtaa
     1921 caatatgcga acaaagcaaa aagtataatt gatgcgtcat agataactct ctttttcgat
     1981 gacgaaatcg ctgagcatgc gtggtgaggt tagtaataga tgggtgctat gttttgccac
     2041 gggtttgtag ctgtgaaacc tggaaaaaaa taagaattaa aaatatttaa agagagttaa
     2101 taaggttctg gttgtaattg tcttggtcaa aaaaaagata ttgtcaatgt tgttttttaa
     2161 attttcaggt tttggttaaa tatttgcttg caaatatagg tgatttttta ttgtaggctt
     2221 ttgaaggcta ttataactgt gcgctaaatt aatgcgttga ataaaagaaa taacaaaatt
     2281 aagaaaagcc atatttacga tatgggcaac ataaatataa tctaaaaaag agtaaggcgt
     2341 tgtaggttgg ttccgcgttc attaccttgt tagtgaacgc ggatttatca gttgtcgaaa
     2401 tggtttttat aataatgaaa aattttaatg tactaaccaa agtcgtattt tgcttgtcgt
     2461 ttttatcaag cgcatttccc acctatgctg cggatgatgt caaagtgact caaaacactt
     2521 cagtttttaa actgaagctt agtgcaacgc gattaatcta tgaacaaggg tcggatggcg
     2581 cagcgataac tgtgacaaat ccgcaggatt ggccgatgct tgtgcagagt aaagtctttg
     2641 aagaagataa agtaactgca gcgcccttta ttgtcactcc gccactgttc aggcttaatc
     2701 caggccagca aaataaaatg tgggttgtgc agactgcgcc agctttacaa gacagggaaa
     2761 aattacactg gctatgcgcc accggcatta cgcctaaaaa gagtgatgtt tgggccgaga
     2821 aagataaaga aaaaattgct gaaaatgtga acctgaacat ccaggttaac gtacatacct
     2881 gcataaaact tattaccagg ccatctggta ttgaaggcaa gatggttaat gcgagtgaga
     2941 agttatcgtg gaaaaagcag ggtgataaaa ttgtcgcggt caatcagtcg ccgttctaca
     3001 tcaacctttc ttctttacag gttggcggta gcaagattga gaaactagat tatattgcgc
     3061 cgttttcgca acatgagttt gttatgccaa aaactagcgg ccaggatatt aaatggtctg
     3121 tgattactga tgaaggtggt gagagtcgtt tgtttatatc tcatttgcaa tcataatgta
     3181 ttacattttt attgaatatt tttatgccat gcatattcca tgttttatcg actgtcatta
     3241 attttgataa tggccttact tgcgggtcag ttaagtgccc aggagtggtc atttgattct
     3301 tcacagttgg aaggaaacgt tagtgcggac actgtggcaa tgtttaatca gggagagcaa
     3361 ctccccggaa attatcgtgt ggaaatttat cttaacggtg aaaaagttga tgttggggag
     3421 tttccctttc atcgtccgga atcccctgaa gaaaaagagc ttgttccatg tttaaccgtt
     3481 gatgacctta tccactatgg cattaaaatt gataaaagca gcagtgatac tgacaataaa
     3541 aagaatcagt gttttaaatg gaactctatt gaaggtttga aagtaaatta tgattttgat
     3601 agccagcgcg tacagataac cgttccacaa ctgtatctgc aagataaaaa aagtagttta
     3661 gcaccagtgt cgttatggaa tgagggtgta gctgcattcc gcatggtgta tcagacaaat
     3721 attgatattt ccaaacaaaa tgataatcaa tctaccacgc gtaattctcg ttatggcagg
     3781 ttcactccag ggtttaatct tggtgcctgg aggtttcgta gttcagtgac atggagcaag
     3841 gaacttggcc agtcagaaag atggcaaagg ggttacatgt ggtttgagcg aggcataaat
     3901 gcgattaaaa gccgattaac gcttggagaa tcttatacgt cgtctgaggt gtttgacagc
     3961 ataccttttc gcggcgggat gttagcgacc gacgatgcga tgactccgcc ggaggacagc
     4021 tactacacac ctgtggtgca tggaatagca caaagtgagg cgcaggttat tattaaacaa
     4081 aatgg
//
LOCUS       DAJTRC010000197         4042 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.201, whole genome shotgun sequence.
ACCESSION   DAJTRC010000197 DAJTRC010000000
VERSION     DAJTRC010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.201"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(886..1077)
                     /locus_tag="OO357_004004"
     CDS             complement(886..1077)
                     /locus_tag="OO357_004004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000165985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
                     /protein_id="HCR5888744.1"
                     /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLYDH
                     ADELYRSCLARFGDDGENL"
     gene            1142..1465
                     /locus_tag="OO357_004005"
     CDS             1142..1465
                     /locus_tag="OO357_004005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000956000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HCR5888745.1"
                     /translation="MLTIRVTDDEHARLLERCEGKQLAVWMRRVCLGEPVARSGRLPT
                     LAPPLLRQLAAIGNNLNQAARKVNSGQWSSGDRVQVVAALMAIGDELRRLRLAVREQG
                     TRDDS"
     gene            1407..2165
                     /locus_tag="OO357_004006"
                     /pseudo
     CDS             1407..2165
                     /locus_tag="OO357_004006"
                     /inference="COORDINATES: protein motif:HMM:NF015397.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
     gene            2143..2673
                     /locus_tag="OO357_004007"
     CDS             2143..2673
                     /locus_tag="OO357_004007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001673158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeB family mobilization protein"
                     /protein_id="HCR5888746.1"
                     /translation="MSNLLQTGAEFEKKLKERAESTEKMLSDEFRKLEESVSKAVTSN
                     ETKIKDAIALFTASTEESLKKHREGVKEAMMQHRKDVLKLAGNTGMMLLGMVIFLFTV
                     SGGTLWYLGGMIQANLEEIRKQNESLEKLNAKTWGVRYHEGSNGRFLVLPEGMKAETN
                     WTMENGKQNAVRLVQE"
     gene            2680..2913
                     /locus_tag="OO357_004008"
     CDS             2680..2913
                     /locus_tag="OO357_004008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000137438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MbeD family mobilization/exclusion protein"
                     /protein_id="HCR5888747.1"
                     /translation="MTELESHLLNALEQLQQDYMQRLNEWESAFVELQKMFSLTQRDN
                     AMLNERVMQLSQQVLHLSEQTERLSQLYSENWR"
     gene            complement(3459..3851)
                     /locus_tag="OO357_004009"
     CDS             complement(3459..3851)
                     /locus_tag="OO357_004009"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888748.1"
                     /translation="MALSACFRQQLNPVVNSLLKERIIHSPKELEHDNAVIRVLQKLQ
                     IQEVCNSTLWETAKQQLLQKSDGRYFAINSKHLAFPGPGESHAFCCIKYKNAIGINGN
                     NAETQSTHYQPYPYDKVSIWGPFPHNLT"
BASE COUNT          966 a          917 c         1207 g          952 t
ORIGIN      
        1 aaaaaaacca ccgctaccaa cggtggtttg tttgccggaa tctagagcta ccaactcttt
       61 cttccgaagg taactggctt ggtggagcac agataccaaa tgctgtcctt ctagtgtagc
      121 cgcagttagg ccaccacttc aagaactctt aatatctcaa tccaccttgt ccagttacca
      181 atggctgctg ccagtggcgt taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccaa cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcggtt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccatcggcg cggccttctt cctgttcctg gtcttttgct cacatgttct ttccggcgtt
      601 atcccctgat tctgtggata acagcatttc gtttttgagt gagtagatac cgctcgccgc
      661 agtcgaacga ccgagcgtag cgagtcagtg agcgaggaag cggaaaagcg cctggacgtg
      721 cattttctcc ttacgcatct gtgcggcatt tcacacccgg tatggcgcac ttttcataca
      781 atccgcactg atgccgcatg gttaagccag tatacactcc gctatcgcta cgtgactggg
      841 tcatggctgc gccccgacac ccgctaacac ccgctgacaa gcccgtcaga ggttttcacc
      901 gtcatccccg aagcgagcga ggcaactgcg gtaaagctca tcggcgtggt cgtaaagcga
      961 ttcacagatg tctgcctgtt catccgcgtc cagttcgttg agcttctcca gcagcgttaa
     1021 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga
     1081 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgcgagag
     1141 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gctgtgaggg
     1201 taaacagctg gcggtatgga tgcggcgggt ctgcctgggt gagccggttg cccgttccgg
     1261 gaggctgccg acactggcac cgccgttact gcgtcagctg gccgccatcg ggaataacct
     1321 gaatcaggca gcgaggaagg tgaacagcgg gcagtggtct tccggtgacc gggttcaggt
     1381 ggtggccgca ctgatggcca tcggggatga gctgcgccgg ctgcgtctgg ccgtcaggga
     1441 acaggggacg cgggatgata gttaagtttc atgccagggg aaaaggcggt ggcagtggtc
     1501 cggttgatta cctgctggga agggagcgta accgggaagg ggcaacggtg cttcagggta
     1561 atccggaaga agtccgggaa ctcatcgatg ccacgccatt tgcgaagaaa tacacgtccg
     1621 gtgttctctc gttcgcggag aaggagctgc cgccgggagg gcgtgaaaaa gtgatggcga
     1681 gctttgagcg tgtactgatg cccggtctcg aaaaaaatca gtacagcatt ctgtgggtgg
     1741 agcaccagga caaggggcgg cttgagctga attttgtcat tccgaacatg gagctacaga
     1801 ccggaaaacg cctccagccg tactacgacc gcgcagacag gccgagaatt gatgcctggc
     1861 agacgctggt gaatcaccat tacgggctgc atgacccgaa cgccccggag aaccgccgga
     1921 cgctgactct ccctgataac ctgccggaaa cgaaacaggc gcttgctgag agcattacac
     1981 ggtggataga cgctctttat catgtcgggg agataaaagg ccgtcaggat gtgattcagg
     2041 cgctcacaga ggcggggctt gaagtggtca gggtgacgcg aagcagtatc agcattgcag
     2101 acccgaacgg cgggaagaat atcagactga aaggagcatt ctatgagcaa tcttttacag
     2161 acgggcgcgg agttcgagaa aaagctgaaa gagagagccg aatctacaga gaaaatgctg
     2221 agcgacgagt tcaggaagct cgaagaatct gtaagcaagg ctgtgacatc aaacgagacg
     2281 aaaatcaaag acgctatagc cctgttcaca gcttcgacag aggaatcact gaaaaaacat
     2341 cgggaagggg tgaaagaggc gatgatgcag cacaggaagg acgtgttaaa gctggcaggg
     2401 aatacgggca tgatgttact ggggatggtc attttcctgt ttaccgtgag tggcgggacg
     2461 ctctggtatc ttggcgggat gatacaggcg aacctggaag aaatcaggaa gcagaacgag
     2521 agtctggaga agctgaacgc gaagacatgg ggcgtgcgtt atcacgaggg gagcaacgga
     2581 cgttttctgg tgttgcccga ggggatgaaa gccgaaacga actggacgat ggaaaacggg
     2641 aaacagaatg ccgtaaggct ggtacaggag tgacagagca tgacggagct ggaaagccat
     2701 ttgctgaacg cattagagca gctgcaacag gactatatgc agcggctgaa cgaatgggag
     2761 agcgccttcg tggaattgca gaaaatgttt tcgcttacgc aacgggacaa cgcgatgctg
     2821 aacgagcggg tcatgcagtt gagtcagcag gtgctgcact tgagcgagca gacagaacgc
     2881 ttgagccagt tatacagcga gaactggaga taagagagga acggttgata caggagcgcg
     2941 aacatgcgtt attcctggaa cgggaacgcc agccggaaat acaggaacgg acactggatg
     3001 gcccttcgct gggatggtaa tcagagacag gctaaaatgt tgtcagcgcg tttccggtgc
     3061 attttttggg ataatggttg gttcgggggc accgggaacg cgcttcagtc agaccttgct
     3121 gaaaacacag gaacgagtta aaacgcatac gggcgcgatt ctggtgtgtc ttttgtcgct
     3181 gtgttttgcc tctattgcca gatcgcgctc attcccctga acttctgttt tcccgctcag
     3241 gtccccgtct tcttcgcttc cgaaagcatg aataaaatcc gtttcagctg tctctggctg
     3301 gcaattcctg cgtgattcac atggctgcat agctatgcat gtggttaaaa tttaccgggt
     3361 gcgatcgcgg cagtttttcg ggtggtttgt tgccattttt acctgtctgc tgccgtgatc
     3421 gcgccgaacg cgtttcagcg gtgcgtacaa ttaagatatt acgttaaatt gtgagggaat
     3481 ggtccccata tactgacttt atcataggga tagggttggt agtgggtcga ttgggtttcg
     3541 gcattgtttc cgttaatgcc aattgcattt ttgtatttga tgcaacagaa ggcgtgagat
     3601 tctcctgggc ctggaaaggc taagtgttta gaattgatgg caaaatatct tccgtccgat
     3661 ttttgaagta gttgttgttt ggctgtctcc caaagggtgc tgttacaaac ttcttgaatt
     3721 tgtagttttt gcaggactct tattactgcg ttgtcatgtt ccaattcttt aggggagtgt
     3781 attattctct cttttaataa gctgtttacc acagggttta gttgttgtct aaagcacgca
     3841 gataatgcca ttgctacaca attttgttcc cgaggaagca tgtttggcgg ggtatgccgt
     3901 gtttgatgtg atatgtctat tctcatagaa ggtctctggc ttatagcatc aacaaatacg
     3961 gctataaaac aaaattttca tagagtgatt caagatcttc ttgagatcct tttttgcggc
     4021 gcgtaatctg ctgcctgcaa ac
//
LOCUS       DAJTRC010000198         3936 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.202, whole genome shotgun sequence.
ACCESSION   DAJTRC010000198 DAJTRC010000000
VERSION     DAJTRC010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3936
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.202"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(348..995)
                     /gene="ychE"
                     /locus_tag="OO357_004010"
     CDS             complement(348..995)
                     /gene="ychE"
                     /locus_tag="OO357_004010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAAT family transporter YchE"
                     /protein_id="HCR5888749.1"
                     /translation="MIQTLFDFPVYFKFFIGLFALVNPVGIIPVFISMTSYQTAAARN
                     KTNLTANLSVAIILWISLFLGDTILQLFGISIDSFRIAGGILVVTIAMSMISGKLGED
                     KQNKQEKSETAVRESIGVVPLALPLMAGPGAISSTIVWGTRYHSISYLFGFFVAIALF
                     ALCCWGLFRMAPWLVRVLRQTGINVITRIMGLLLMALGIEFIVTGIKGIFPGLLN"
     gene            1472..>3936
                     /gene="adhE"
                     /locus_tag="OO357_004011"
                     /gene_synonym="adhC"
     CDS             1472..>3936
                     /gene="adhE"
                     /locus_tag="OO357_004011"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HCR5888750.1"
                     /translation="MAVTNVAELNALVERVKKAQREYASFTQEQVDKIFRAAALAAAD
                     ARIPLAKMAVAESGMGIVEDKVIKNHFASEYIYNAYKDEKTCGVLSEDDTFGTITIAE
                     PIGIICGIVPTTNPTSTAIFKSLISLKTRNAIIFSPHPRAKDATNKAADIVLQAAIAA
                     GAPKDLIGWIDQPSVELSNALMHHPDINLILATGGPGMVKAAYSSGKPAIGVGAGNTP
                     VVIDETADIKRAVASVLMSKTFDNGVICASEQSVVVVDSVYDAVRERFATHGGYLLQG
                     KELKAVQDVILKNGALNAAIVGQPAYKIAELAGFSVPENTKILIGEVTVVDESEPFAH
                     EKLSPTLAMYRAKDFEDAVEKAEKLVAMGGIGHTSCLYTDQDNQPARVSYFGQKMKTA
                     RILINTPASQGGIGDLYNFKLAPSLTLGCGSWGGNSISENVGPKHLINKKTVAKRAEN
                     MLWHKLPKSIYFRRGSLPIALDEVITDGHKRALIVTDRFLFNNGYADQITSVLKAAGV
                     ETEVFFEVEADPTLSIVRKGAELANSFKPDVIIALGGGSPMDAAKIMWVMYEHPETHF
                     EELALRFMDIRKRIYKFPKMGVKAKMIAVTTTSGTGSEVTPFAVVTDDATGQKYPLAD
                     YALTPDMAIVDANLVMDMPKSLCAFGGLDAVTHAMEAYVSVLASEFSDGQALQALKLL
                     KEYLPASYHEGSKNPVARERVHSAATIAGIAFANAFLGVCHSMAHKLGSQFHIPHGLA
                     NALLICNVIRYNANDNPTKQTAFSQYDRPQARRRYAEIADHLGLSAPGDRTAAKIEKL
                     LAWLETLKAELGIPKSIREAGVQ"
BASE COUNT         1093 a          959 c          900 g          984 t
ORIGIN      
        1 aattcaatca aatagagatt ggctaagcat gtctatcttt tctgattaca gttccagtag
       61 tgaaatgcac aataatttaa caattgatta ttatttagca ctttcctcta ccaaaggcag
      121 tggaattact aatatcattt caattattct tcagcaagcc caggattatg atgtggcgaa
      181 aataacatga tggtgaggag aagcagttaa ggaaatcctt ataactaact gtaatttgag
      241 aagaatattt ttacctattc cttttgcttt tgataactgc gcgggaacaa agtaaaaata
      301 atgacgaagt aaacggagca tggcagctcc gtttcatttg aaaggaatta attaagcagg
      361 ccggggaaaa tccccttaat accagtaacg ataaattcaa tccccaatgc catcagcaat
      421 agccccataa tacgcgtaat cacgttgatg ccggtctggc gtaaaacccg taccagccac
      481 ggtgccatgc ggaacaatcc ccagcaacat aaagcgaaca atgcaatagc cacaaagaaa
      541 ccaaacagat agctaatgct gtgataacgc gtaccccaga cgatggtaga actgatcgcc
      601 cctggccccg ccatcaacgg caacgccaat ggcaccacac caatactttc acgtaccgcg
      661 gtttctgatt tttcttgctt gttctgttta tcctcgccaa gcttgccgct gatcatcgac
      721 atcgctatcg tcaccaccag gatgcccccg gcgatacgga acgaatcaat tgatatacca
      781 aaaagttgta gaatcgtgtc gccgagaaaa agcgagatcc acaagataat ggccacagac
      841 aggttggctg taaggttagt tttgtttcgc gctgccgctg tctgataact ggtcatgctg
      901 ataaagacgg gaataatccc taccgggttg accagcgcaa ataacccgat gaaaaatttg
      961 aagtaaacgg gaaaatcaaa aagggtctga atcacggttc gctccgaagc aaaagccgga
     1021 taatgttagc cataaataag gttgaaaaga cgcgctgaca atacgccttt tgacagcatt
     1081 tttcacctcc taactactta aaattgctat cattcgttat tgttatctag ttgtgcaaaa
     1141 catgctaatg tagccaccaa atcatactac aatttattaa ctgttagcta taatggcgaa
     1201 aagcgatgct gaaaggtgtc agctttgcaa aaatttgatt tggatcacgt aatcagtacc
     1261 cagaagtgag taatcttgct tacgccacct ggaagtgacg cattagagat aataactcta
     1321 atgtttaaac tcttttagta aatcacagtg agtgtgagcg cgagtaagct tttgattttc
     1381 atcggttaag caaatcatca ccgcactgac tatactctcg tattcgagca gatgatttac
     1441 taaaaaagtt taacattatc aggagagcat tatggctgtt actaatgtcg ctgaacttaa
     1501 cgcactcgta gagcgtgtaa aaaaagccca gcgtgaatat gccagtttca ctcaagagca
     1561 agtagacaaa atcttccgcg ccgccgctct ggctgctgca gatgctcgaa tcccactcgc
     1621 gaaaatggcc gttgccgaat ccggcatggg tatcgtcgaa gataaagtga tcaaaaacca
     1681 ctttgcttct gaatatatct acaacgccta taaagatgaa aaaacctgtg gtgttctgtc
     1741 tgaagacgac acttttggta ccatcactat cgctgaacca atcggtatta tttgcggtat
     1801 cgttccgacc actaacccga cttcaactgc tatcttcaaa tcgctgatca gcctgaagac
     1861 ccgtaacgcc attatcttct ccccgcaccc gcgtgcaaaa gatgcaacca acaaagcggc
     1921 tgatatcgtt ctgcaggctg ctatcgctgc cggtgctccg aaagatctga tcggctggat
     1981 cgatcaacct tctgttgaac tgtctaacgc actgatgcac cacccagaca tcaacctgat
     2041 cctcgcgact ggtggtccgg gcatggttaa agccgcatac agctccggta aaccagctat
     2101 cggtgtaggc gcgggtaaca ctccagttgt tattgatgaa actgctgata tcaaacgtgc
     2161 agttgcatct gtactgatgt ccaaaacctt cgacaacggc gtaatctgtg cttctgaaca
     2221 gtctgttgtt gttgttgact ctgtttatga cgcagtacgt gaacgtttcg caacccacgg
     2281 cggctatctg ttgcagggta aagagctgaa agctgttcag gatgttatcc tgaaaaatgg
     2341 tgcgctgaac gcggctatcg ttggtcagcc agcctataaa attgctgaac tggcaggctt
     2401 ctctgtacca gaaaacacca agattctgat cggtgaagtg accgttgttg atgaaagcga
     2461 accgttcgca catgaaaaac tgtccccgac tctggcaatg taccgcgcta aagatttcga
     2521 agacgcggta gaaaaagcag agaaactggt tgctatgggc ggtatcggtc atacctcttg
     2581 cctgtacact gaccaggata accaaccggc tcgcgtttct tacttcggtc agaaaatgaa
     2641 aacagctcgt atcctgatta acaccccggc ttctcagggt ggtatcggtg acctgtataa
     2701 cttcaaactc gcaccttccc tgactctggg ttgtggttcc tggggtggta actccatctc
     2761 tgaaaacgtt ggtccgaaac acctgatcaa caagaaaacc gttgctaagc gagctgaaaa
     2821 catgttgtgg cacaaacttc cgaaatctat ctacttccgc cgtggctccc tgccaatcgc
     2881 gctggatgaa gtgattactg atggccacaa acgtgcgctc atcgtgactg accgcttcct
     2941 gttcaacaat ggttatgctg atcagatcac ttccgtactg aaagcagcag gcgttgaaac
     3001 tgaagtcttc ttcgaagtag aagcggaccc gaccctgagc atcgttcgta aaggtgcaga
     3061 actggcaaac tccttcaaac cagacgtgat tatcgcgctg ggtggtggtt ccccgatgga
     3121 cgccgcgaag atcatgtggg ttatgtacga acatccggaa actcacttcg aagagctggc
     3181 gctgcgcttt atggatatcc gtaaacgtat ctacaagttc ccgaaaatgg gcgtgaaagc
     3241 gaaaatgatc gctgtcacca ccacttctgg tacaggttct gaagtcactc cgtttgcggt
     3301 tgtaactgac gacgctactg gtcagaaata cccgctggcg gactatgcgc tgaccccgga
     3361 tatggcgatt gtcgacgcca acctggttat ggacatgccg aagtccctgt gtgctttcgg
     3421 tggtctggac gcagtaactc acgccatgga agcttatgtt tctgtactgg catctgagtt
     3481 ctctgatggt caggctctgc aggcactgaa actgctgaaa gaatatctgc cagcgtccta
     3541 ccacgaaggg tctaaaaatc cggtagcgcg tgaacgtgtt cacagtgcag caactatcgc
     3601 gggtatcgca tttgcgaacg ccttcctggg tgtatgtcac tcaatggcgc acaaactggg
     3661 ttcccagttc catattccgc acggtctggc aaacgccctg ctgatttgta acgttattcg
     3721 ctacaatgcg aacgacaacc cgaccaagca gactgcattc agccagtatg accgtccgca
     3781 ggctcgccgt cgttatgctg aaattgccga ccacctgggt ctgagcgcac cgggcgaccg
     3841 tactgctgct aagatcgaga aactgctggc atggctggaa acgctgaaag ctgaactggg
     3901 tattccgaaa tctatccgtg aagctggcgt tcagga
//
LOCUS       DAJTRC010000199         3736 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.204, whole genome shotgun sequence.
ACCESSION   DAJTRC010000199 DAJTRC010000000
VERSION     DAJTRC010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3736)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3736)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3736
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.204"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(88..276)
                     /locus_tag="OO357_004012"
     CDS             complement(88..276)
                     /locus_tag="OO357_004012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000874242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1737 domain-containing protein"
                     /protein_id="HCR5888751.1"
                     /translation="MAFKHYDVVRAAPPSDLAEKLTHKLKEGWQPFGSPVAITPYTLM
                     QAIAAEGDVVVSGATEPE"
     gene            536..871
                     /gene="iraM"
                     /locus_tag="OO357_004013"
     CDS             536..871
                     /gene="iraM"
                     /locus_tag="OO357_004013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-adapter protein IraM"
                     /protein_id="HCR5888752.1"
                     /translation="MKWIVIDTVIQPSCGISFSVIWSKIKLIIWYQSDAFLPPESIFT
                     LTHTGIMLNNKVLPVTIYNVVPFNKTFWNLIKNSQECPTNTDNVLNECFNNRCTLQIC
                     PYGLKQQSP"
     gene            complement(1152..1260)
                     /locus_tag="OO357_004014"
                     /pseudo
     CDS             complement(1152..1260)
                     /locus_tag="OO357_004014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001416037.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3927 family protein"
     gene            complement(1783..1859)
                     /locus_tag="OO357_004015"
     tRNA            complement(1783..1859)
                     /locus_tag="OO357_004015"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1823..1825),aa:Arg,seq:tct)
     gene            complement(1961..2036)
                     /locus_tag="OO357_004016"
     tRNA            complement(1961..2036)
                     /locus_tag="OO357_004016"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2001..2003),aa:Ile,seq:cat)
     gene            complement(2181..3002)
                     /locus_tag="OO357_004017"
     CDS             complement(2181..3002)
                     /locus_tag="OO357_004017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitermination protein"
                     /protein_id="HCR5888753.1"
                     /translation="MKLEDLPKYYSPKSPGLTDASASTSKDALSITDVMAAQGMTQNR
                     AEMGFSAFLGKMGISMNDRARATELLADYALSRCDRVAALRKLPAEIKPVVMRIMASY
                     AFEDYARSAASKKQCPCCYGEKFIESIVFTNKIQYPDGKPPVWAKCTKGVYPFYWEEW
                     KKVREVVKVACPECGGKGEVSTACKDCRGRGVAIHREESVKRGMPVIRDCQRCGGRGY
                     ERLPSTEAFNAICEVTNQITRASWEKTVKKFYDALVTRFDIEEAWAERQLKKVTR"
     gene            complement(3017..3373)
                     /locus_tag="OO357_004018"
     CDS             complement(3017..3373)
                     /locus_tag="OO357_004018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001459679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5888754.1"
                     /translation="MLIDLVLPYPPTVNTYWRRRGSTYFISEEGKRYRRAVALIVRQQ
                     RLKLSLSGRLAIKVIAEPPDKRRRDLDNILKAPLDALTHAGVLMDDEQFDEINIVRGQ
                     PVSGGRLGVKIYPIMH"
     gene            complement(3386..>3736)
                     /locus_tag="OO357_004019"
     CDS             complement(3386..>3736)
                     /locus_tag="OO357_004019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5888755.1"
                     /translation="PQQEKTVVSIAVDPESPESFMKRPKRRRWVNEKYKRWVKTQPCA
                     CCGKPADDPHHLIGHGQGGMGTKSHDIFTLPLCREHHNELHADPLAFEEKHGSQVDLI
                     FRFLDHAFATGVLG"
BASE COUNT         1061 a         1003 c          718 g          954 t
ORIGIN      
        1 taatatccgt cagcgtcagt gcatttagtc ccactgccag actgtctttt atggcctgga
       61 atgccgggcc agtacgatga cgtagtatca ctccggctca gttgcaccac tgaccaccac
      121 atcaccttct gctgcaatcg cctgcatcag ggtataaggg gttatggcca ccggactacc
      181 aaacggctgc cagccctctt tcagtttatg tgtcagcttt tccgcaagat ctgacggcgg
      241 cgccgccctg acaacatcat agtgtttaaa tgccatggtt ctttccaaca tctgaaaaat
      301 aattctttaa aatacctgac atgtaataca gaaaaaacac aaaaccatac cttaaataaa
      361 aacctgatta tcaagcagat atgcatggat aaactacaag acgagatata aaccaccctg
      421 catttaaata aacaataaac aacatcagaa aaataattct gttctatggt ttaattcaaa
      481 aatatcattt atacttttca gaacatcacc agcaaggcat aaacaaggaa actaaatgaa
      541 gtggattgtg attgatacag ttatccagcc atcatgcgga atatcttttt cagtcatatg
      601 gagtaaaata aaattaataa tctggtatca atcggatgct ttcttacctc ctgaaagtat
      661 atttacactg actcacacag gcatcatgct caataacaaa gtgctacctg taaccattta
      721 caacgtagta ccattcaata aaacattctg gaatttaatc aaaaacagcc aggaatgccc
      781 tacaaataca gataacgtat tgaatgaatg ctttaataac cgttgcactc tgcaaatatg
      841 cccttatgga ctaaaacaac aaagtccata aggagtttac tcacatctga caaaatcaat
      901 ataaacagcc cctccggaga ggggctggag agtggcgcta tgtgccattc catggtgccg
      961 ggtgcctccc ggtgaattca gtaccagcac ctgaatccgc gattatccca tatacctact
     1021 cgctgattgc ccctccgcac agggggattc accatgccag tttcttttaa caaactcccc
     1081 gcaaaccaga caacagtcaa ccgcctgaat tgtgaagtat ttaaaaattt ctcccgctaa
     1141 ctgatacccg gctaacagtc tggcgttttc tttttcagca acgggaaagc aacaaccacc
     1201 acacccgcca ccagcacacc gtcagccggc actgacatta tccggctgct gcaatgccat
     1261 taacaaaaac agtaagcaat cactttttac cgtaacaggt gataatccag atatgtatct
     1321 accccagatg agtaatccga agttcatcca taccacaggt cctggctatt ctgttgtact
     1381 cctgaacaag agcaaataat tctgaattag caaccatgaa ctcatcgcaa accctctgta
     1441 tagcatcact attcagaata ataacgtctc ttcccgaaag acgatcagga gtacagaaca
     1501 aaactgtcaa acggctgaag gcctttgctc gtgctgcatt gactatatca atacgctgcc
     1561 taaggatgaa acaccccgac gcctcatcaa tattcactct acccacacca tatgaatgat
     1621 aaatacttaa tgctgaaaaa accattagac cgtataacaa atactcaatc aatacttaac
     1681 agaactttta tttttgacaa atatataata ttttcaacaa tatcctgagc caggtatatt
     1741 tcagtataag gctctgccgg aaggaatctg gaagaatgaa tatggcgcgc tgtactggat
     1801 tcgaaccagt gaccgattgc ttagaaggca attgctctgt ccggctgagc taacaacgct
     1861 gaataccgat aatggaccgc catcggggac ccgccccccg caccaacaac cctgttatcg
     1921 tgtcgtctgc tcttcctgat aagctaatgg cggtttgtga tggtggccct tgctggattt
     1981 gaaccagcga cctggcgatt atgagtcgct cgctctcacc actgagctaa agggccggga
     2041 gcagaataat aacggtccgt aattaatttc gcaataaaaa aacccgctca gcggcgggtt
     2101 gtagaaactc ttctaacgcc aggcataaaa cgcccatcat tatgacgaat ttaccacaga
     2161 ttccggaaaa atcaaccttg ttacctagtt acctttttta actgccgctc agcccatgct
     2221 tcttcaatat caaaccgggt caccagcgca tcataaaatt tcttaactgt tttttcccat
     2281 gacgcgcgtg ttatctggtt tgtcacctcg catatagcat taaatgcctc cgttgatggt
     2341 agtctttcat agccacgacc accacaacgc tggcagtctc tgataacagg cataccacgt
     2401 tttaccgact cttcacgatg aatggcgaca ccacgcccac ggcaatcctt acaggcggtg
     2461 gaaacctcac cctttccgcc acactccgga caggcaactt ttaccacctc cctgactttt
     2521 ttccattctt cccagtaaaa cggatacaca cctttcgtac actttgccca taccggcggc
     2581 ttaccatccg gatactggat cttgtttgta aaaactatgc tttcaataaa tttttcccca
     2641 tagcaacaag ggcactgctt tttactcgct gcgctgcggg cataatcctc aaaagcgtac
     2701 gaagccataa tgcgcatcac taccggtttt atttctgccg gaagttttct caacgccgcc
     2761 acacgatcgc accgactgag tgcataatct gccagtaatt ctgttgcccg cgccctgtca
     2821 ttcatactga tgcccatttt ccccaggaac gcagaaaaac ccatctcagc ccgattctgt
     2881 gtcatgccct gcgcggccat cacatcagtg atactcagcg catcttttga cgttgaggcc
     2941 gatgcatcgg tcaggccagg ggattttggg gagtagtatt tcggtaaatc ttccagtttc
     3001 attttttgac ctgctcttaa tgcattatgg ggtaaatctt cacccccaga cgtccaccag
     3061 atactggctg accacgaacg atattgattt catcaaactg ctcatcgtcc attaacactc
     3121 ccgcatgcgt cagcgcatcc agcggtgctt tcaggatatt gtccaggtcg cgacgacgct
     3181 tatccggtgg ctctgcaatc acctttatcg ccagccttcc ggacaggctt aatttcagcc
     3241 gctgctggcg aacaataagc gccacagccc ggcgataacg ctttccctcc tccgagataa
     3301 aatatgtgct gccacggcgt cgccagtaag tgttcaccgt cggcgggtaa ggtaaaacca
     3361 aatctatgag catcagtcac ctcttttacc caagcacgcc agttgcaaag gcgtgatcaa
     3421 gaaaacgaaa aattaaatca acctgagaac catgcttttc ttcgaacgcc agaggatccg
     3481 catgaagctc gttgtgatgc tcccgacaca gcggtagcgt gaaaatatcg tgagatttgg
     3541 tccccattcc gccctgacca tgaccaatca ggtgatgggg atcgtcggct ggcttaccac
     3601 aacacgcaca cggctgtgtc ttcacccagc gtttgtattt ctcgttaacc cagcggcgac
     3661 gtttaggtcg tttcatgaaa gattccggag actcaggatc aacggcaatg ctgaccaccg
     3721 tcttttcctg ttgtgg
//
LOCUS       DAJTRC010000200         3689 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.205, whole genome shotgun sequence.
ACCESSION   DAJTRC010000200 DAJTRC010000000
VERSION     DAJTRC010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.205"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..223
                     /locus_tag="OO357_004020"
     CDS             <1..223
                     /locus_tag="OO357_004020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_052672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HCR5888756.1"
                     /translation="FQRDRYGRILGQVYAPDGMNINQFMVRAGAAWVYEQYNTDPVLP
                     VLQNEARQQKRGLWSDADPVPPWIWRHRK"
     gene            269..478
                     /gene="hha"
                     /locus_tag="OO357_004021"
     CDS             269..478
                     /gene="hha"
                     /locus_tag="OO357_004021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_958726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin expression modulator Hha"
                     /protein_id="HCR5888757.1"
                     /translation="MAKTKQEWLYQLRRCSSVNTLEKIIHKNRDSLSNSERESFNSAA
                     DHRLAELITGKLYDRIPKEIWKYVR"
     gene            517..1107
                     /locus_tag="OO357_004022"
     CDS             517..1107
                     /locus_tag="OO357_004022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2726 domain-containing protein"
                     /protein_id="HCR5888758.1"
                     /translation="MKLIIFILIVLIIAALLIRIILRSVNQHSPLLMQLHAAGIRTGD
                     AERILSSGEYWQRQKTLLTEREVSFMKGLFRIVDMKRWYLCPQVRVADIVQLNGNIRP
                     RSRQWWQLFRMVSQWHVDVVIVELRSFSIVAAVELDDASHLRPERRRRDILLEEVLRQ
                     AGIPLLRSHDARKLLQMTGEWLNTTGADQQSPEHRS"
     gene            1347..1607
                     /gene="repA"
                     /locus_tag="OO357_004023"
     CDS             1347..1607
                     /gene="repA"
                     /locus_tag="OO357_004023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication regulatory protein RepA"
                     /protein_id="HCR5888759.1"
                     /translation="MSQTENAVTSSLSQKRFVRRGKPMTDSEKQMAAVARKRLTHKEI
                     KVFVKNPLKDLMVEYCEREGITQAQFVEKIIKDELQRLDILK"
     gene            1831..1905
                     /gene="tap"
                     /locus_tag="OO357_004024"
     CDS             1831..1905
                     /gene="tap"
                     /locus_tag="OO357_004024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001370046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RepA leader peptide Tap"
                     /protein_id="HCR5888760.1"
                     /translation="MPGKVQDFFLCSLLLRIVSAGWCD"
     gene            1898..2755
                     /gene="repA"
                     /locus_tag="OO357_004025"
     CDS             1898..2755
                     /gene="repA"
                     /locus_tag="OO357_004025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858393.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="incFII family plasmid replication initiator
                     RepA"
                     /protein_id="HCR5888761.1"
                     /translation="MTDLQQTYYRQVKNPNPVFTPREGARTLPFCGKLMEKAVGFTSR
                     FDFAIHVAHARSLGLRRRMPPVLRRRAIDALLQGLCFHYDPLANRVQCSITTLAIECG
                     LATESAAGKLSITRATRALTFLAELGLITYQTEYDPLIGCYIPTDITFTSALFAALDV
                     SEEAVAAARRSRVEWENRQRKKQGLDTLGMDELMAKAWRFVRERFRSYQTELKSRGMK
                     RARARRDADRQRQDIVTLVKRQLTREISEGRFTASREAVKREVERRVKERMILSRNRN
                     YSRLATASP"
     gene            3456..3647
                     /locus_tag="OO357_004026"
     CDS             3456..3647
                     /locus_tag="OO357_004026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001352029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888762.1"
                     /translation="MAENGYGLAGLGMGKVKSVNQYRLTPGFGGFTPVSHVTTACRLP
                     CRWRGIRIIQAAFNAFAKV"
BASE COUNT          997 a          901 c          929 g          862 t
ORIGIN      
        1 ttttcagcga gatcgttatg gtcgtattct cgggcaggtt tatgcaccag acggaatgaa
       61 tattaatcag tttatggtcc gggccggggc tgcctgggta tatgagcagt acaacacaga
      121 tcccgtgcta cctgtccttc agaacgaagc cagacaacaa aaacgtgggc tctggtcaga
      181 tgctgatccg gttcccccct ggatatggag gcaccgtaaa taaagcgatg gcaatcctat
      241 cattcatctg ttctcagcgg gaataaaaat ggcaaaaaca aaacaggaat ggctgtatca
      301 gttacggcga tgttcatcag tcaatacgct ggaaaaaatt attcataaaa acagagacag
      361 tttatcaaac agtgaacgtg aatcatttaa ttcagcagca gaccaccggc tagcggaatt
      421 aatcaccgga aagttatatg acaggatacc aaaagagata tggaaatatg taaggtaata
      481 attcagcaat aattaattgc caggagcaga taaataatga agttgattat ttttattctc
      541 attgttctga ttattgcagc gctacttatc cggattatcc tcagaagtgt aaatcagcat
      601 tctcctttgc tgatgcaatt gcatgcggca ggtatacgga ctggtgatgc agagcgaata
      661 ctgtccagcg gtgaatactg gcaacgtcag aagacgctgc tgacagaaag ggaagtcagt
      721 tttatgaaag gactgttcag aattgtggat atgaagcggt ggtatctgtg tccgcaggta
      781 cgggtagcgg atatcgtcca gctgaacggg aatatccggc cacgatcacg ccagtggtgg
      841 cagttattca ggatggtgtc tcagtggcat gttgatgtgg ttatcgttga gctgcgttcg
      901 ttcagtattg ttgcagcagt agagctggat gatgccagcc atttacgacc ggaacgcaga
      961 cgccgggata ttcttctgga agaggttctg aggcaggctg gtattccgtt gctcagaagc
     1021 cacgatgcca gaaaactgct gcagatgacc ggagaatggc tgaatacaac aggggctgat
     1081 cagcagtcac cggaacatcg tagctgacgc cttcgcgttg ctcagttgtc caaccccgga
     1141 aacgggaaaa agcaagtttt cctcgctccc ggcgtttcca taactgaaaa ccatactatt
     1201 tcacagttta aatcacatta aacgacagta atccccgttg atttgtgcgc caacacagat
     1261 cttcgccaca attctcaagt cgctgatttc aaaaaactgt agtatcctct gcgaaacgat
     1321 ccctgtttga gtattgagga ggcgagatgt cgcagacaga aaatgcagtg acttcctcat
     1381 tgagtcaaaa gcggtttgtg cgcagaggta agcctatgac tgactctgag aaacaaatgg
     1441 ccgctgttgc aagaaaacgt cttacacaca aagagataaa agtttttgtc aaaaatcctc
     1501 tgaaagatct catggttgag tactgcgaga gagaggggat aacacaggct cagttcgttg
     1561 agaaaatcat caaagatgaa ctgcagagac tggatatact aaagtaaaga ctttactttg
     1621 tggcgtagca tgctagatta ctgatcgttt aaggaatttt atggctggcc acgccataag
     1681 gtggcaggga actggttctg atgtggatgt acaggagcca gaaaagcaaa aaccccgata
     1741 atcttcttta actttggcga gtcagaaaga ttaccggggc ccacttaaac cgtatagcca
     1801 acaatcaagc tatgcgggga gtatagttat atgcccggaa aagttcaaga cttctttctg
     1861 tgctcgctcc ttctgcgcat tgtaagtgca ggatggtgtg actgatcttc aacaaactta
     1921 ttaccgccag gtaaagaacc cgaatccggt gttcacaccc cgtgaaggtg cccggacgct
     1981 tccgttctgc gggaaactga tggaaaaggc ggtgggcttc acctcccgtt ttgatttcgc
     2041 cattcatgtg gcacatgccc gttcgcttgg tctgcgtcgg cgcatgccac cggtactacg
     2101 tcgccgggcc attgatgcgc tgctgcaggg gctgtgtttt cactatgacc cactggccaa
     2161 ccgcgtccag tgttccatca ccacgctggc cattgagtgc ggactggcga cggagtctgc
     2221 tgccggaaaa ctctccatca cccgggccac ccgggccctg acgttccttg cagagctggg
     2281 actgattacc taccagacgg aatatgaccc gcttatcggg tgctacattc cgaccgatat
     2341 cacgttcaca tctgcactgt ttgctgccct cgatgtatca gaggaggcag tggccgccgc
     2401 gcgccgcagt cgtgtggaat gggaaaacag acagcgcaaa aagcaggggc tggataccct
     2461 gggtatggat gaactgatgg cgaaagcctg gcgttttgtt cgtgagcgtt tccgcagtta
     2521 ccagacagag cttaagtccc gtggaatgaa gcgtgcccgt gcgcgtcgtg atgcggacag
     2581 gcagcgtcag gatattgtca ccctggtgaa acggcaactg acgcgtgaaa tctcggaagg
     2641 gcgcttcact gccagtcgtg aggcggtaaa acgtgaagtg gagcgtcgtg tgaaggagcg
     2701 catgattctg tcacgtaacc gtaattacag ccggctggcc acagcttccc cctgaaagtg
     2761 acctcctcag aataatccgg cacgcgccgg aggcattcgc acgcctgaag cccgccagtg
     2821 cacaaaaaat cagcgctaca tacaaaaaac aacctcatca tccaccttca ggtgcatccg
     2881 gtcccccctg tttttgatac aaaacacgcc tcacagacgg ggaattttgc ttatccacat
     2941 taaactgcaa gggacttcac cataaggtta caaccgttca cgtcataaag cgccagacgt
     3001 caaccctaca gggtgcaatg tatcttttaa acacctgttt atatctcctt taaactactt
     3061 aattacattc atttaaaaag aaaacctgtt cactgcctgt cctgtggaca gacaaatatg
     3121 cacctcccac cgcaagcggc gggcccccaa ccgaagccgg tttagttaca acatgcaaac
     3181 ataaacccct aaaaaaccca agtccaacgc aaaactgaaa ccacaacgcc cccctcataa
     3241 ctgaaaagcg ccgccgcccc cgcccaaagg gcgggaacag agtcgctttt aattatgaat
     3301 gttgtaacta catcatcatc gctgtcagtc ttctggctgg acgtatcgaa tacacgctcg
     3361 taagcggccc tcacggcccg ctaacgcgga gatacgcccc gacttcgggt aaaaccctcg
     3421 tcgggaccac tccgaccgcg cacagaagct ctatcatggc tgaaaacggg tatggcttgg
     3481 cagggctggg gatgggtaag gtgaaatctg tcaatcagta ccggctgacg ccgggcttcg
     3541 gcggttttac tccggtatca catgtaacca cagcgtgccg ccttccatgc cgctggcgcg
     3601 gcatcagaat aatacaagca gcatttaatg catttgcaaa agtgtgagta atattaagtg
     3661 gtcgtttcag ggatatcgtt aagacttga
//
LOCUS       DAJTRC010000201         3563 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.206, whole genome shotgun sequence.
ACCESSION   DAJTRC010000201 DAJTRC010000000
VERSION     DAJTRC010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3563)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3563)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3563
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.206"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            119..1282
                     /locus_tag="OO357_004027"
     CDS             119..1282
                     /locus_tag="OO357_004027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5888763.1"
                     /translation="MSLFRRGEIWYASFTLPNGKRFKQSLGTKDKRQATELHDKLKAE
                     AWRVSKLGEIPDITFEEACVRWLEEKAHKKSLDDDKSRIGFWLQHFAGMQLRDITESK
                     IYSAMQKMTNRRHEENWRLRAEACRKKGKPVPEYTPKPASVATKATHLSFIKALLRAA
                     EREWKMLDKAPIIKVPQPKNKRIRWLEPHEAQRLIDECPEPLKSVVEFALATGLRRSN
                     IINLEWQQIDMQRRVAWINPEESKSNRAIGVALNDTACRVLKKQIGNHHRWVFVYKES
                     CTKPDGTKAPTVRKMRYDANTAWKAALRRAGIDDFRFHDLRHTWASWLVQAGVPLSVL
                     QEMGGWESIEMVRRYAHLAPNHLTEHARQIDSILNPSVPNSSQSKNKEGTNDV"
     gene            1631..2803
                     /locus_tag="OO357_004028"
     CDS             1631..2803
                     /locus_tag="OO357_004028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase"
                     /protein_id="HCR5888764.1"
                     /translation="MHMIEINSLLLITSVILMSLLAVGLFDKISPINLVEHGRNNQID
                     GMRGFLAIFVLIHHAAIWNGYLSSGVWEAPSSNLLANLGQVGVSFFFMITGYLFFSKI
                     ISGDQDWTRLYVSRLLRLTPMFIVSLCLIFIIVGFKSGWRMQVSTEELFVSIMKWLPF
                     TALGMPNINDVKDSFTINAAVTWTLVYEWFFYFSLPVISALIKRKVSIYMVMISAISL
                     FVFILFFSKIHIASFLFGLLAFLLNKSKIVNGIAKAKVTPIIITAIMVFEMTYFKTTY
                     APLPLILCGITFIIIASGCDLYGILRLNITRKLGETTYSVYLLHGIFLYCLMTWIIPN
                     NYTENTFIILVSTTAFLITFTSCLTFKLIETPFIKLTKQTTTLVKELIPTLTNNNQ"
     gene            complement(2857..3480)
                     /locus_tag="OO357_004029"
     CDS             complement(2857..3480)
                     /locus_tag="OO357_004029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005060548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5888765.1"
                     /translation="MVLNNPYFEANAGGADLAIDNTGTRPVTVVINGGNFHRVSSARY
                     THTNIQVTSSGGGKVTVILNGTTFQSAGDYQPSAEQPYWITGANCEVVDIGCVFTETT
                     SKVTSASALSVTRSGKINANGSIDVATGVSSVNVVSTGVYDVSFSHPLAAVASGYIVQ
                     ITPISAPDSVSCDVTYIGVDTFRVTLRNTLSGAGISSSFAFSITRLI"
BASE COUNT         1125 a          748 c          729 g          961 t
ORIGIN      
        1 atcccactaa gaataaatct ccttacctca caacacgaca agcctgcatt gcggctcttc
       61 agtctccgct gcatactgtc caggtgagcg cgggtgatgg cataacagag gaaagaaaat
      121 gtcactcttc cgcagaggtg aaatatggta cgccagtttc acattgccga acggtaaaag
      181 atttaaacag tctcttggaa caaaggacaa aaggcaggcg acagaactcc atgacaagct
      241 aaaggctgaa gcatggcggg tcagcaaact tggtgaaata cctgatataa cgttcgagga
      301 agcgtgtgtc aggtggcttg aagagaaagc acataaaaaa tcactggacg atgacaaaag
      361 ccggatcgga ttctggcttc aacatttcgc aggaatgcaa ctaagagaca ttactgaatc
      421 aaaaatttat tcagcaatgc agaaaatgac gaaccggcgt catgaggaaa actggagact
      481 cagggcagaa gcatgcagaa aaaaagggaa acctgttcca gaatacacgc caaaaccagc
      541 gtccgttgca acgaaggcta cgcatctttc atttataaag gccctactaa gagccgcaga
      601 gcgtgaatgg aaaatgctgg ataaggcacc aattattaaa gtgcctcaac caaagaataa
      661 acggatccgc tggctggagc cccatgaagc acaaaggctg attgatgaat gtccggagcc
      721 attaaagtct gttgttgaat ttgcactggc aacaggctta agacgctcga acatcatcaa
      781 ccttgaatgg caacaaatag atatgcagcg ccgggtggca tggataaacc cggaagagag
      841 taaatcaaac cgcgcaattg gcgttgcgct gaatgatact gcatgtcgcg tattgaaaaa
      901 acaaatcggg aatcatcacc gttgggtatt tgtgtacaag gaaagctgta ccaaaccaga
      961 cggaacgaaa gcgccaacgg tcaggaagat gcggtatgac gcaaacacag cctggaaagc
     1021 ggcgctgaga cgagcaggta ttgatgattt cagatttcac gacttgagac acacctgggc
     1081 aagttggctg gttcaagccg gagtcccgtt gtcagtgtta caggaaatgg gaggctggga
     1141 gtctatcgaa atggttcgtc gatatgctca ccttgcacct aatcacctta ccgaacacgc
     1201 acggcaaata gactcgatcc tgaacccatc ggtcccaaat tcgtcccagt caaaaaataa
     1261 ggaaggtact aatgatgtgt aacttattga tttaaatggt gccgataata ggagtcgaac
     1321 ctacgacctt cgcattatga atctgtagca ccaatcataa ctatctgttt tagcaagcat
     1381 taaccgcatt cactaagcta tagttgatga cacaaacaga aagttgatgc atgatgttgc
     1441 catatatatg tcacaaatac ggcacaacga tcttcaaaca tatagccaca cattcacaga
     1501 agagcacaaa gccttgcaat ccagtgcaaa gcttggtgtg tctccgtttt gtttcatcag
     1561 ttacacataa ctatatgatc attaactaaa agtcagtata cttggagaaa ttttaattgc
     1621 gtaatagagc atgcatatga ttgaaattaa cagtttatta ttaataacat ccgtgatatt
     1681 gatgtcgtta ttagctgtgg ggttatttga taaaatttcc ccaataaatc ttgttgagca
     1741 cggcaggaat aatcaaatag atggaatgcg ggggttctta gcaattttcg tgcttattca
     1801 tcacgcagca atttggaatg gatacttgtc atctggagta tgggaagcac cttcatcaaa
     1861 tctgttagca aatttaggcc aagttggtgt gtcattcttt tttatgatta ctggttatct
     1921 gttcttttca aagattatct ctggagatca ggactggaca aggctttatg tatcaagatt
     1981 actacgatta accccaatgt tcatagttag cctatgtctc atttttatca ttgtaggttt
     2041 taagtctgga tggagaatgc aggtatccac agaagagctt tttgtgtcaa taatgaagtg
     2101 gttgccattc actgcgctag gtatgccgaa cattaatgac gtaaaagatt catttactat
     2161 caatgccgct gtaacatgga cacttgtata tgaatggttc ttttattttt ctcttccggt
     2221 aatttccgcg ctcataaaaa gaaaagtcag tatttacatg gttatgatta gcgcaatatc
     2281 gctatttgtt ttcattttat ttttcagtaa aatacacata gcatcatttc tttttgggct
     2341 cctggcattt ttgctaaata aatcaaagat agtcaatgga attgccaaag caaaagtaac
     2401 accaattata ataactgcga taatggtttt tgaaatgacg tatttcaaaa caacttacgc
     2461 accgctacca ctgattcttt gtggaataac atttatcatt atcgcatcag gttgtgattt
     2521 gtatggaata ttaagattaa atataaccag aaagctagga gaaactacct acagcgttta
     2581 ccttctgcat gggatattcc tttattgcct aatgacgtgg attattccta ataattacac
     2641 tgaaaataca tttatcatac tggtaagcac aactgcattt cttattacgt tcacatcatg
     2701 cctaactttt aaattaatag agacgccatt catcaaatta acaaaacaaa ccacaacttt
     2761 agtaaaagaa ttaataccca cattaacaaa caacaatcaa taaacaataa acataagccg
     2821 ccctacatat atgtccaaat ggggcggcga atgtatttat attagtctgg taatgctaaa
     2881 tgcaaacgaa ctggagattc ctgccccgct aagcgtattg cgcagggtca ccctaaatgt
     2941 atctacgcca atataagtaa catcacaact aactgaatca ggcgcagata taggtgttat
     3001 ctgcacaata taaccgctag caactgctgc gagcggatga gaaaagctaa catcatacac
     3061 acctgtagaa accacgttaa ccgatgaaac accagtagca acgtcgatac taccattggc
     3121 gttaattttt cccgagcgtg tgactgacaa tgcagaagcc gatgttacct tactggttgt
     3181 ttctgtaaaa acacacccaa tgtcaactac ctcgcaatta gctcctgtta tccagtatgg
     3241 ttgttctgca cttggctgat aatcccctgc gctctggaac gttgtgccgt tgagtatgac
     3301 tgtaactttt cccccacctg agcttgttac ttgaatattc gtgtgcgtgt atcttgcact
     3361 gctcacgcga tgaaaatttc caccgtttat aaccacagtt acagggcgcg tcccagtatt
     3421 atctatagca aggtctgccc ccccggcatt agcctcaaag tatgggttgt ttaataccaa
     3481 agcgcacgaa ccgttaaggc catttatagc taattgcgca ccacctgtac tggaatcccc
     3541 ctgagtacca tttccttcac aag
//
LOCUS       DAJTRC010000202         3425 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.207, whole genome shotgun sequence.
ACCESSION   DAJTRC010000202 DAJTRC010000000
VERSION     DAJTRC010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.207"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..367)
                     /gene="azoR"
                     /locus_tag="OO357_004030"
     CDS             complement(<1..367)
                     /gene="azoR"
                     /locus_tag="OO357_004030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HCR5888766.1"
                     /translation="MSKVLVLKSSILAGYSQSNQLSDYFVEQWSEKHSADEITVRDLA
                     ANPIPVLDGELVGALRPSDAPLTPRQQEALALSDELIAELKAHDVIVIAAPMYNFNIS
                     TQLKNYFDLVARAGVTFRYT"
     gene            568..>3425
                     /gene="hrpA"
                     /locus_tag="OO357_004031"
     CDS             568..>3425
                     /gene="hrpA"
                     /locus_tag="OO357_004031"
                     /EC_number="3.6.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RNA helicase HrpA"
                     /protein_id="HCR5888767.1"
                     /translation="MTEQQKLTFTALQQRLDSLMLRDRLRFSRRLHCVKKVKNPDAQQ
                     AIFQEMAKEIDQAAGKVLLREAARPEITYPDNLPVSQKKQDILEAIRDHQVVIVAGET
                     GSGKTTQLPKICMELGRGIKGLIGHTQPRRLAARTVANRIAEELKTEPGGCIGYKVRF
                     SDHVSDNTMVKLMTDGILLAEIQQDRLLMQYDTIIIDEAHERSLNIDFLLGYLKELLP
                     RRPDLKIIITSATIDPERFSRHFNNAPIIEVSGRTYPVEVRYRPIVEEADDTERDQLQ
                     AIFDAVDELSQESPGDILIFMSGEREIRDTADALNKLNLRHTEILPLYARLSNSEQNR
                     VFQSHSGRRIVLATNVAETSLTVPGIKYVIDPGTARISRYSYRTKVQRLPIEPISQAS
                     ANQRKGRCGRVSEGICIRLYSEDDFLSRPEFTDPEILRTNLASVILQMTALGLGDIAA
                     FPFVEAPDKRNIQDGVRLLEELGAITTDEQASAYKLTPLGRQLSQLPVDPRLARMVLE
                     AQKHGCVREAMIITSALSIQDPRERPMDKQQASDEKHRRFHDKESDFLAFVNLWNYLG
                     EQQKALSSNAFRRLCRTDYLNYLRVREWQDIYTQLRQVVKELGIPVNSEPAEYREIHI
                     ALLTGLLSHIGMKDADKQEYTGARNARFSIFPGSGLFKKPPKWVMVAELVETSRLWGR
                     IAARIDPEWVEPVAQHLIKRTYSEPHWERAQGAVMATEKVTVYGLPIVAARKVNYSQI
                     DSALCRELFIRHALVEGDWQTRHAFFRENLKLRAEVEELEHKSRRRDILVDDETLFEF
                     YDQRISHDVISARHFDSWWKKVSRETPDLLNFEKSMLIKEGAEKISKLDYPNFWHQGN
                     LKLRLRYQFEPGADADGVTVHIPLPLLNQVEESGFEWQIPGLRRELVIALIKSLPKPV
                     RRNFVPAPNYAEAFLGRVTPLELPLLDSLERELRRMTGV"
BASE COUNT          835 a          896 c          929 g          765 t
ORIGIN      
        1 cggtatagcg gaaagtaacg cctgcgcgtg caaccaggtc aaaatagttt ttcaactgag
       61 ttgagatgtt gaagttatac atcggtgccg caataacgat aacgtcgtgg gctttcagct
      121 cggcaatcag ctcatcggaa agcgccagag cttcctgctg acgcggagtc agcggcgcat
      181 cgctcggacg cagagcgcca accagttcgc catccagtac cggaatcgga tttgcagcta
      241 ggtcgcgaac ggtgatttca tcagcggagt gcttttcgct ccattgttca acaaaataat
      301 cggacaactg attagactga gagtaccctg ccaggatgct ggatttaaga actaatacct
      361 tgctcatggt gtttccttat agatgtttga atggacgatg ccccgttgct tgctgacact
      421 ttattcacaa tcctgccaca gagatcgcgc aataaatcga agtctatgtt cgaatttatt
      481 gaacaacgca tagaaagccg cgatgtggta ctctatatct atcatttaaa agaaaattaa
      541 tcaggcagac tactgcccac taacgttatg acagaacaac aaaaattgac ctttactgcc
      601 ttgcaacagc ggctggattc gctgatgctg cgcgacagac tgcgtttttc tcgccgtctg
      661 cattgtgtta agaaggttaa aaatcctgat gcacaacagg ccattttcca ggagatggcg
      721 aaagagattg accaggcggc agggaaagtc ctgctgcgtg aagcggcacg accggaaatc
      781 acttatcctg acaatttacc ggttagtcag aaaaaacagg acatactcga agcgattcgt
      841 gatcaccagg tggtgatcgt cgccggggaa accggctccg gtaaaacgac tcagctaccg
      901 aaaatctgta tggagctggg gcgcggtatt aaagggctga tcggtcatac ccagccgcgt
      961 cgcctggcgg cacgaacggt ggccaaccgt attgcggaag agctgaaaac ggagccgggc
     1021 ggttgcatcg gttacaaagt gcgtttcagc gatcacgtaa gtgataacac gatggtcaag
     1081 ctgatgaccg acgggatcct gctggcggag atccagcaag accgcctgct gatgcagtac
     1141 gacaccatca ttattgacga agcgcacgaa cgcagcctga atatcgattt tctgctcggc
     1201 tatttgaaag agttgctgcc gcggcgtcct gacctaaaaa tcattatcac ttccgcgact
     1261 atcgacccgg aacgcttttc gcgccacttt aataatgcgc cgattattga agtctccggt
     1321 cggacctatc cggtggaagt gcgctatcgc ccgattgttg aagaagccga tgacaccgag
     1381 cgcgatcagt tgcaggcgat ttttgacgcc gtagacgaac taagccagga aagccccggc
     1441 gacattctga tctttatgag cggcgagcgg gaaattcgcg ataccgccga tgcgctgaac
     1501 aagctgaact tacgccatac cgaaatcttg ccgctttatg cgcggctttc gaacagcgaa
     1561 cagaaccgcg tgttccagtc gcacagcgga cggcgcattg tgctggcgac caacgtcgcg
     1621 gaaacctcgc tgaccgtacc ggggattaaa tacgtcatcg accccggtac agcgcgtatc
     1681 agccgctaca gctatcgcac caaagtgcag cgtttgccga ttgaacccat ttcgcaggcg
     1741 tctgccaatc agcgtaaagg ccgctgtggt cgtgtgtccg aagggatctg tattcgtctc
     1801 tattccgaag acgatttcct ctcgcgcccg gagtttaccg atccggagat tctgcgtacc
     1861 aacctggcct cggttatttt gcagatgacc gcgctggggc tgggcgatat cgctgcgttc
     1921 ccgtttgtcg aagcaccgga taaacgcaat atccaggatg gcgtgcgtct gctcgaagaa
     1981 ctgggcgcga tcaccacgga tgaacaggcc agcgcctata aactgacgcc gctcggtcgc
     2041 cagctctcgc agttgcctgt cgacccacgt ctggcgcgta tggtgctgga agcgcaaaaa
     2101 catggctgcg tgcgtgaggc gatgattatc acgtccgcgc tctccatcca ggatccgcgc
     2161 gaacgtccga tggacaaaca gcaggcatcg gacgaaaaac atcgtcgctt ccacgacaaa
     2221 gaatccgact tcctcgcgtt tgtaaatctg tggaattatc ttggcgagca gcaaaaggcg
     2281 ctttcttcca acgccttccg tcgcctgtgt cgtaccgatt acctcaacta tctgcgcgtg
     2341 cgcgaatggc aggatatcta cacccagttg cgccaggtgg tgaaagaact tggcattccg
     2401 gtcaacagcg aaccggcgga gtatcgtgaa attcacatcg ccttactgac cggtttgctt
     2461 tcccatatcg gcatgaaaga tgccgataaa caggaatata ccggtgcacg taacgcgcgt
     2521 ttctccatct tccccggttc tggtttattc aaaaagccgc cgaaatgggt aatggtggcg
     2581 gaactggtag aaaccagccg cctgtggggg cgcattgctg cgcgtatcga cccggaatgg
     2641 gtagaacccg ttgctcagca tttgattaaa cgcacctaca gcgaaccgca ctgggaacgg
     2701 gcgcagggcg cggtgatggc aacggaaaaa gtcactgttt atggtttgcc gattgttgct
     2761 gcgcgcaagg tcaactacag ccagatcgat tcggcgttat gtcgtgaact ctttattcgc
     2821 cacgctctgg tggaaggtga ctggcagacg cgtcacgcat tcttccgtga aaacctgaaa
     2881 ctgcgggccg aagtggaaga gctggaacac aaatcacgtc gccgcgatat tctggttgat
     2941 gacgaaacgc tgtttgagtt ctacgaccag cgcatcagcc acgatgtaat ctccgctcgt
     3001 cacttcgata gctggtggaa aaaagtcagc cgcgaaacgc ctgatttgct caactttgaa
     3061 aagagcatgt tgatcaaaga aggggcggaa aaaatcagca agctggatta cccgaacttc
     3121 tggcatcagg gcaatctcaa gctgcgtttg agatatcagt ttgagccagg cgcggacgcg
     3181 gacggtgtga ccgtacatat tccgctgccg ttacttaacc aggttgagga aagcgggttt
     3241 gaatggcaga tccccggtct gcgccgcgaa ttggtgattg ctctgattaa atcgttgccg
     3301 aaaccggtac gccgtaattt tgtgcctgca ccaaactacg ccgaagcgtt tttaggccgc
     3361 gtcacaccgc tggagttacc gttgctcgac agccttgagc gcgagttacg gcggatgacc
     3421 ggcgt
//
LOCUS       DAJTRC010000203         3418 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.208, whole genome shotgun sequence.
ACCESSION   DAJTRC010000203 DAJTRC010000000
VERSION     DAJTRC010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3418)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3418)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3418
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.208"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..208
                     /locus_tag="OO357_004032"
     CDS             <1..208
                     /locus_tag="OO357_004032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888768.1"
                     /translation="VIKRALYHNAAAVVLAHNHPSGEVTPSKADRLITERLVQALGLV
                     DIRVPDHLIVGGNQVFSFAEHGLL"
     gene            271..492
                     /locus_tag="OO357_004033"
     CDS             271..492
                     /locus_tag="OO357_004033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF987 domain-containing protein"
                     /protein_id="HCR5888769.1"
                     /translation="MRIITRGEAMRIHQQHPASRLFPFCTGKYRWHGSAEAYTGREVQ
                     DIPGVLAVFAERRKDSFGPYVRLMSVTLN"
     gene            655..1029
                     /locus_tag="OO357_004034"
     CDS             655..1029
                     /locus_tag="OO357_004034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system YeeU family
                     antitoxin"
                     /protein_id="HCR5888770.1"
                     /translation="MSDKLSGITHPDDNHDRPWWGLPCTVRPCFGARLVQEGNRLYYL
                     ADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSGELNPRYQHTVTLYAKGLTCEAD
                     TLGSCGYVYLAVYPTPAPATTS"
     gene            1076..1450
                     /locus_tag="OO357_004035"
     CDS             1076..1450
                     /locus_tag="OO357_004035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxin"
                     /protein_id="HCR5888771.1"
                     /translation="MKTLSDTHVREVSRCPSPVTIWQTLLIRLLDQHYGLTLNDTPFV
                     DERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGFSTCPRSQLINSIDILRARRATG
                     LMTRDNYRTVNNITLGKYPEAK"
     gene            1447..1938
                     /locus_tag="OO357_004036"
     CDS             1447..1938
                     /locus_tag="OO357_004036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309434.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888772.1"
                     /translation="MMKLALTLEADSVNVQALNIGRIVVDVDGIELAELINMVCDNGY
                     SLRVVDESDQTSAECTPPFATLTGIRCSTAHITETDNAWLYSLSHQTSDFGESEWIHF
                     TGSGYLLRTDAWSYPVLRLKRLGLSKTFRRLVVTLIRRYGVSLIHLDASAECLPGLPT
                     FDW"
     gene            1950..2147
                     /locus_tag="OO357_004037"
     CDS             1950..2147
                     /locus_tag="OO357_004037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888773.1"
                     /translation="MKSLTTETALDILIAWLQDNIDCGSGIIFDNDEDKTDSAALLPC
                     IEQAREDVRTLRHLQLLHQNR"
     gene            2232..2792
                     /locus_tag="OO357_004038"
                     /pseudo
     CDS             2232..2792
                     /locus_tag="OO357_004038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309436.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4942 domain-containing protein"
     gene            2864..>3418
                     /locus_tag="OO357_004039"
     CDS             2864..>3418
                     /locus_tag="OO357_004039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001179707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS110-like element ISSfl8 family transposase"
                     /protein_id="HCR5888774.1"
                     /translation="MQNITLIGIDLGKHSFHIHCQDKSGKALLRKKFTRTKLMQFLAT
                     CPPSVVVMEACAGAHFMARCISDFGHETKLISPQFVRPFVKSNKNDFVDAEAICEAAS
                     RPSMRFVQPRTETQQAMRALHRVRESLIRDKVKTTNQIHGFLLEFGISLPIGDAVIKR
                     LSLVLAEHEIPEYLSRLLVRLHTHY"
BASE COUNT          849 a          915 c          836 g          818 t
ORIGIN      
        1 agtgattaaa cgcgccctgt accacaatgc cgctgccgtg gtgctggcgc acaatcaccc
       61 gtccggtgaa gtcacaccca gtaaggcaga ccggcttatc accgaacgtc tggtacaggc
      121 actgggcctg gtggatatcc gggtgccgga ccatctgata gtcggtggca accaggtttt
      181 ctcctttgcc gaacatggtc tgctttaacc cgtcacaacc acatcacacc tgttttcact
      241 tttatcttct gtcttcagag gtatcacatc atgagaatca tcacccgtgg tgaagccatg
      301 cgtattcacc aacagcatcc tgcatcccgt ctttttccgt tctgtaccgg taagtatcgc
      361 tggcacggca gtgctgaagc gtatactggt cgtgaagtgc aggatattcc cggcgttctt
      421 gccgtgtttg cagaacgccg taaggacagt tttggcccgt atgtccggct gatgagtgtc
      481 accctgaact gatgttccgt cacccgggaa acgagtacga ccagatgacc tcagacgccg
      541 ctgtggatga ggccattctc atcaatgcat acatcttcac cgaaaccggt cagcgcgccg
      601 gctgacctgt catcgttaaa ccattcttaa ccacttttca gagaggattt tatcgtgtca
      661 gacaaactct ccgggataac tcatcccgat gacaatcacg accgcccctg gtgggggctt
      721 ccctgcacag tgaggccctg ttttggcgct cgtctggtgc aagagggtaa ccgcctgtat
      781 taccttgccg accgcgccgg tatcagaggc cgcttcagcg acgcagatgc ataccatctg
      841 gaccaggcct ttccgctgct gatgaaacaa ctggaactca tgctcaccag cggtgaactg
      901 aatccccgat atcagcatac cgtcacgctg tatgcaaaag ggctgacctg cgaagccgac
      961 accctcggct cctgtggtta cgtttatctg gctgtttatc cgacaccagc acccgcaacc
     1021 acctcataac cccgaagctc tcacctttag cactaccagt tacagagaac ttaagatgaa
     1081 aacattatcc gacacacatg tacgggaggt atctcgctgc ccgtctcccg tcaccatctg
     1141 gcagacactg ctcatccgac tgctggacca gcattatggc ctcacactga atgacacacc
     1201 gtttgtcgat gaacgtgtga ttgagcagca tattgaggca ggcatttcac tgtgtgatgc
     1261 agttaatttt ctcgtggaaa aatacgcgct ggtgcgtacc gaccagccgg gattcagcac
     1321 ctgtccccgc tctcagttaa taaacagtat tgatatcctc cgggctcgcc gggcgaccgg
     1381 cctgatgacc cgcgacaatt acagaacggt aaataacatt accctgggta agtatccgga
     1441 ggcgaaatga tgaaactggc cctgacactg gaagccgaca gcgttaacgt acaggcactg
     1501 aacatagggc gcattgtcgt tgacgtcgat ggtattgagc tcgctgaact gattaacatg
     1561 gtctgcgata acggctactc ccttcgtgtt gttgatgaat ctgaccagac ctcagcagaa
     1621 tgcacgccac catttgctac ccttaccggc atacgctgca gtaccgcaca tatcacggaa
     1681 acggacaacg cctggttgta ctcgctgtca caccagacca gtgacttcgg tgaatcagaa
     1741 tggattcatt ttacaggtag cggatatctg ttacgtaccg atgcgtggtc atacccggtt
     1801 ctgcggctta aacgcctggg gctgtcaaaa acgttccgtc gtctggtcgt cacactcatc
     1861 cggcgttatg gcgtcagtct cattcatctg gatgccagcg ctgaatgcct gccgggttta
     1921 cccactttcg actggtaacc aggaacaaca tgaaatcatt aaccacggaa acagcgctgg
     1981 atattctgat tgcgtggctg caggacaata tcgactgcgg atcgggaatt atctttgaca
     2041 acgatgagga taaaacggat tcagcagcac tgttgccctg tatcgaacag gcccgggagg
     2101 atgtccgtac cctgcgtcat ctgcagcttc tgcaccagaa ccggtgaagc tcactcacca
     2161 gacttcattc ctctgacgcc agcctgcaca gggctggcgt tttcatttat ctgcaaaaag
     2221 gaatatcgat tatgtcagat atcacaatct cccgtccgga agtggtcaac gggcatacgg
     2281 acgttatctg ctcaaccagc attcgccaca ttctggctgt acgaaagagt acactgttgc
     2341 aaatcgacac acttatccga caactggctg aaatctcggt gctgacggaa agcatcggag
     2401 gtaaaactgc cccggactgg gccatgaaac aggattttcg ctgcggctgc tggctgatgg
     2461 agaaacccga aacagcaatg aaagccatta cccggaatct tgatcgcgaa atctggcgtg
     2521 acctgatgca acgttccggg atgctgtcat taatggatac gcaggcccgt gatacatggt
     2581 accggtcact ggagtacgat aattttccgg aaatcagcga agcgaacatt ctgagcacat
     2641 ttgaacaact gcaccagaat aaggatgagg tgtttgagcg aggggtgatc aacgtcttca
     2701 gagggctgaa ctggaattac aaaaccaatt gcccctgtaa atttggcagt aaaattatcg
     2761 tcaacaatct ggtgagatgg gaccggtggg gataatgata tggtcacccc caccttaaca
     2821 gcagtacgct gttaaggtaa atccctttaa cggagagacc atcatgcaga acatcacact
     2881 tattggtatc gatctcggaa agcactcctt ccacattcat tgtcaagaca agtctggtaa
     2941 ggctcttctg cgtaaaaaat ttacccgtac taaactgatg cagtttctgg cgacatgtcc
     3001 gccctcagtt gttgtaatgg aagcctgtgc gggtgcacac tttatggctc gctgtatctc
     3061 tgattttggg catgaaacta agctgatttc tccacagttc gttcgcccgt ttgtcaaaag
     3121 taataagaat gattttgttg atgcagaagc catatgcgaa gcggcttcac gaccatcaat
     3181 gcgttttgtc cagccaagaa ctgagaccca gcaggccatg agggcgttac accgggtcag
     3241 agaatcgctc atcagagata aggtgaaaac aaccaaccag atccacggtt tcttgctgga
     3301 atttggaatc agcctgccga taggtgatgc tgtaataaaa cggttgtctc tcgtgttggc
     3361 tgaacacgaa atccccgaat atctgagccg ccttttggtg aggttgcata cccactac
//
LOCUS       DAJTRC010000204         3379 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.209, whole genome shotgun sequence.
ACCESSION   DAJTRC010000204 DAJTRC010000000
VERSION     DAJTRC010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.209"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..1803
                     /gene="betT"
                     /locus_tag="OO357_004040"
     CDS             <1..1803
                     /gene="betT"
                     /locus_tag="OO357_004040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choline BCCT transporter BetT"
                     /protein_id="HCR5888775.1"
                     /translation="LILLSVTFLGLSRYGDIKLGPDHAQPDFSYHSWFAMLFSAGMGI
                     GLMFFGVAEPVMHYLSPPVGTPETVAAAKEAMRLTFFHWGLHAWAIYAIVALILAFFS
                     YRHGLPLTLRSALYPIIGDRIYGPVGHAVDIFAVIGTVFGVATSLGYGVLQVNAGLNH
                     LFGVPINETVQVILIVVITGLATISVVSGLDKGIRILSELNLGLALLLLALVLCLGPT
                     VLLLKSFVENTGGYLSELVSKTFNLYAYEPKSSNWLGGWTLLYWGWWLSWSPFVGMFI
                     ARVSRGRTIREFVTGVLFVPAGFTLMWMTVFGNSAIYLIMNQGATDLANTVQQDVALA
                     LFNFLEHFPFSSVLSFIAMAMVIVFFVTSADSGAMVVDTLASGGVANTPVWQRIFWAS
                     LMGIVAIALLLAGGLSALQTVTIASALPFSVILLISIYGLLKALRRDLTKRESLSMAT
                     IAPTAARNPIPWQRRLRNIAYLPKRSLVKRFMDDVIQPAMTLVQEELNKQGTISHISD
                     AVDDRIRLEVDLGNELNFIYEVRLRGYISPTFALAAMDNDEQQTEQHRYYRAEVYLKE
                     GGQNYDVMGWNQEQLINDILDQYEKHLHFLHLVR"
     gene            1866..3143
                     /locus_tag="OO357_004041"
     CDS             1866..3143
                     /locus_tag="OO357_004041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001505014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2254 domain-containing protein"
                     /protein_id="HCR5888776.1"
                     /translation="MISRWKWMLKQTIKKLWFRATLFAIVAIITALLSILFKSMIPES
                     VSVKVGAEAVDNILNILASSMLAVTTFSLSIMVTAYGSATTNVTPRATRLVVEDVTTQ
                     NVLATFIGSFLFSLVGIIALNMGAYGERGRVILFIVTLVVIALILITLLRWIQHLTSL
                     GRVGETTAQVEQAAIETFIARARNPCLGGYPWLENNEQPKGTVAVYPKKIGYVEYIDM
                     VKLSKLLTNDPRHVYLVAQPGSFIHPSMPVLYLSQGQESSINTDLLETIIVSDARSFA
                     QDPRFCLSVMAEIACRALSPAVNDPGTAIDVIGRGVRILSTYAQNKSDEIEVKYPSVH
                     VAPLQNNDLLEDFFSPVARDGASMREIQIRVLKGLSMLSKGWPEIFAEAAHTLAFETL
                     EHATRADHIDSDRYLIKSTYYNLFSGEYSNKKT"
BASE COUNT          804 a          769 c          872 g          934 t
ORIGIN      
        1 ctgattttac tgagtgtcac gttccttgga ctctcacgct acggtgatat caagctgggc
       61 ccggaccatg cgcagcctga tttcagctac cactcctggt ttgcgatgct tttttcggca
      121 gggatgggga tcggcctgat gttctttggc gttgccgaac ctgtaatgca ttatctttcg
      181 ccacccgttg gcactccaga aaccgttgcg gcagctaagg aagcaatgcg tctgaccttt
      241 ttccactggg gactgcacgc atgggcaatt tatgccattg tggcgctgat tctggcgttc
      301 ttcagttacc gtcacggtct gcctttaact ctgcgctccg cactctatcc cattattggc
      361 gatcgcatat acggacctgt aggacatgcg gttgatattt tcgctgttat aggcacggtc
      421 tttggcgttg cgacatcact gggttacggt gttttgcagg tgaatgccgg tttgaaccat
      481 cttttcgggg tgcccatcaa tgaaacggtg caggtaattc tgatcgtggt catcacgggg
      541 ttagcgacga tttcagtggt gtccggtctg gataagggaa tacgtatcct gtctgaactc
      601 aatctgggtc tggctttgtt gctcctggcg ctggtcctgt gtctgggacc aaccgtgctt
      661 ctgctgaagt catttgtgga aaatacgggc ggttatcttt cggaactggt gagtaaaacg
      721 ttcaaccttt acgcgtatga gcccaagtcg agcaactggc tggggggctg gacattactg
      781 tactggggat ggtggctttc atggtcgccg tttgtgggga tgttcatcgc acgggtctcc
      841 cgcgggcgaa ccattcgcga gtttgtcacc ggcgtgctgt ttgttcccgc gggttttacg
      901 ctaatgtgga tgacggtgtt tggtaacagc gcgatctatc tcattatgaa ccagggggcc
      961 acagacctcg ccaatactgt tcagcaggat gtggcgctgg ccctgtttaa tttcctggag
     1021 catttcccgt tctcttctgt gctgtcattc attgcaatgg cgatggtcat cgtcttcttt
     1081 gtaacgtctg ctgattcggg ggcaatggtt gtggatactc tggcatcagg tggagtggca
     1141 aacacacccg tctggcagcg aatattctgg gcctcgctca tgggcattgt tgcaattgcg
     1201 cttctccttg ccggagggct aagtgcgctg caaacggtga caatagcgag tgcattgccc
     1261 ttctcagtga tcttactaat atctatatac ggacttttaa aagctttgcg ccgggatttg
     1321 accaagcgtg aaagcctgag catggcgaca attgctccta cggctgcacg taacccaatt
     1381 ccttggcaga gaaggttacg caatatcgcg tatctgccga agcgatctct tgtgaaacgt
     1441 tttatggacg acgttatcca gcctgccatg acgctggttc aggaggaact gaacaagcag
     1501 gggacgataa gccacattag tgatgcagtc gacgatcgta ttcgtcttga agtcgatttg
     1561 ggcaacgagc tgaattttat atatgaagtg aggcttcgcg ggtatatctc accgaccttc
     1621 gcgctcgccg caatggataa tgatgagcag cagactgaac aacatcgata ttatcgcgct
     1681 gaggtttatc tcaaagaagg cggtcaaaat tatgatgtga tgggctggaa ccaggaacag
     1741 ctgattaatg acatactgga ccagtacgaa aaacacctgc acttcctgca cctggttcgt
     1801 taatagcaac atgccgtcct gggggcggca attattatcc cggccgcaat atgagggaat
     1861 gcagaatgat ttcacgctgg aaatggatgc tgaagcagac aattaaaaaa ctatggttca
     1921 gggcaacgtt attcgcaatt gtcgcgataa taacggccct tttatcaatt ctttttaaat
     1981 caatgatacc tgagtcggtt tccgtgaagg ttggtgcgga agcagtcgat aacattctga
     2041 acatactggc atcgagtatg ctggcagtga ccacattttc gctgagtatc atggtcacag
     2101 cctacggttc agccactact aatgtgactc ccagagctac gcgtttagtt gttgaagacg
     2161 tcaccacaca aaatgtactg gccaccttca tcggttcttt tctcttcagt ctggtaggga
     2221 ttattgccct caatatggga gcttatggag aaagggggag agtcatttta ttcattgtca
     2281 cactggttgt cattgcctta atcctcatca cattgcttcg ctggatacag catttgacct
     2341 ctctggggag ggttggtgag acaacggcac aagtagaaca ggcggccatc gaaacattta
     2401 ttgcgagagc aagaaatccc tgtctcggtg gatatccatg gcttgagaac aatgaacagc
     2461 cgaaaggaac ggttgcagtt tatccgaaga agattggcta tgttgaatat attgatatgg
     2521 tgaaacttag caagctgctg accaatgatc cccgtcatgt atacctcgtg gcgcagccag
     2581 gcagtttcat acatccgtcc atgccagttt tgtacctgag tcagggccag gagtcgtcaa
     2641 tcaacaccga tttacttgag acgattattg tctcggatgc acgttcattt gctcaggatc
     2701 ctcgattttg tcttagcgtc atggccgaaa tagcctgccg ggccctttcc cctgcagtga
     2761 acgatcctgg aaccgccatt gatgtcattg gcagaggtgt tcgtatactt tccacttacg
     2821 cgcagaataa atctgatgaa atagaagtga aatatccttc agtacatgtt gcaccacttc
     2881 agaataacga tctactggaa gactttttct cacctgtcgc gcgcgatggt gccagtatga
     2941 gggagattca gataagagtc cttaaaggac tgtcgatgct gagtaaaggt tggcctgaga
     3001 tatttgctga agccgcacat accctggcat ttgaaacatt agagcatgca actcgtgctg
     3061 accatataga ttctgataga tatctaataa aatcaactta ttataattta tttagtggcg
     3121 aatattctaa taaaaaaaca tagctgcaga gcaagcaatt cggttgttgg caagtcaagg
     3181 gcaaagcatt cgcgtgagaa tgggcgtatg ctttgtgcca aaagcaaacg ttgctaattc
     3241 tgcactgttc agcgatggcg cagtagagac aggcactgcc actgtccagt cggcaaaagc
     3301 gaacactttg ccactgcggg gataaactga ttgaagctct cgcgcaaaat cacttattct
     3361 tttgcgttaa taaaatgta
//
LOCUS       DAJTRC010000205         3305 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.210, whole genome shotgun sequence.
ACCESSION   DAJTRC010000205 DAJTRC010000000
VERSION     DAJTRC010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.210"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..241
                     /locus_tag="OO357_004042"
     CDS             <1..241
                     /locus_tag="OO357_004042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888777.1"
                     /translation="NGSVRYCQYTSMFGASYRYSGAGVGYQVASVVGGGFTPFIAAAL
                     ITYFAGNWHSIAIYLLAGCLISAMTALLMKDNQRS"
     gene            343..1797
                     /gene="amn"
                     /locus_tag="OO357_004043"
     CDS             343..1797
                     /gene="amn"
                     /locus_tag="OO357_004043"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707888.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HCR5888778.1"
                     /translation="MNNKGSGLTPAQALDKLDALYEQSVVALRNAIGKYITIGELPDE
                     NARKQGLFVYPSLTVTWDGSTTNPPKTRAFGRFTHAGSYTTTITRPTLFRSYLNEQLT
                     LLYQDYGAHISVQPSQHEIPYPYVIDGSELTLDRSMSADLTRYFPTTELAQIGDETAD
                     GIYHPTEFSPLSHFDARRVDFSLARLRHYTGTPVEHFQPFVLFTNYTRYVDEFVRWGC
                     SQILDPDSPYIALSCAGGNWITAETEAPEEAISDLAWKKHQMPAWHLITADGQGITLV
                     NIGVGPSNAKTICDHLAVLRPDIWLMIGHCGGLRESQAIGDYVLAHAYLRDDHVLDAV
                     LPPDIPIPSIAEVQRALYDATKLVSGRPGEEVKQRLRTGTVVTTDDRNWELRYSASAL
                     RFNLSRAVAIDMESATIAAQGYRFRVPYGTLLCVSDKPLHGEIKLPGQANRFYEGAIS
                     EHLQIGIRAIDLLRAEGDRLHSRKLRTFNEPPFR"
     gene            2140..2856
                     /locus_tag="OO357_004044"
     CDS             2140..2856
                     /locus_tag="OO357_004044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HCR5888779.1"
                     /translation="MGRKWANIVAKKTAKDGATSKIYAKFGVEIYAAAKQGEPDPELN
                     TSLKFVIERAKQAQVPKHVIDKAIDKAKGGGDETFVQGRYEGFGPNGSMIIAETLTSN
                     VNRTIANVRTIFNKKGGNIGAAGSVSYMFDNTGVIVFKGTDPDHIFEILLEAEVDVRD
                     VTEEEGNIVIYTEPADLHKGIAALKAAGITEFSTTELEMIAQSEVELSPEDLEIFEGL
                     VDALEDDDDVQKVYHNVANL"
BASE COUNT          932 a          715 c          713 g          945 t
ORIGIN      
        1 aaatggtagt gtccgctatt gccagtacac ctcaatgttt ggtgccagtt atcgctacag
       61 tggtgctgga gtcggttatc aggttgccag tgtggttggc ggtggattta caccttttat
      121 tgccgctgca ctcatcactt actttgccgg gaactggcat agcatcgcca tttatttgct
      181 ggctggatgc ctgatttctg caatgaccgc tttattgatg aaagacaatc aacgctcgtg
      241 atagcctggc gaagatcatc cgatcttcgc cttatacttt tgtttcacat tcctgtgaca
      301 tactatcgga tgtgcggtaa ttgtatggaa caggagacac acatgaataa taagggctcc
      361 ggtctgaccc cagctcaggc actggataaa ctcgacgcgc tgtatgagca atctgtagtt
      421 gctttacgca acgccattgg caaatatatt acaattggcg aattacctga tgaaaacgcc
      481 cgtaaacaag gtctttttgt ctatccatca ctgaccgtaa cctgggacgg tagcacaaca
      541 aatcccccca aaacgcgcgc atttggtcgt tttactcacg caggcagcta caccaccacg
      601 attactcgcc ctactctctt tcgttcgtat ctgaatgagc aactaacgtt gctttatcag
      661 gattatggtg cgcatatctc tgtgcaaccc tcgcaacatg aaatccctta tccttatgtc
      721 atcgatggct ctgaattgac acttgatcgc tcaatgagcg ctgatttaac tcgctacttc
      781 ccgacaacag aactggcgca aatcggcgat gaaactgcag acggcattta tcatccaacg
      841 gaattctccc cgctatcgca ttttgatgcg cgccgcgtcg atttttccct cgcacggttg
      901 cgccattata ccggtacgcc agtagaacat tttcaaccgt tcgtcttgtt taccaactac
      961 acacgttatg tggatgaatt cgttcgttgg ggatgcagcc agatcctcga tcctgatagt
     1021 ccctacattg ccctttcttg tgctggcggg aactggatca ctgccgaaac cgaagcgcca
     1081 gaagaagcca tttccgacct tgcatggaaa aaacaccaga tgccagcatg gcatttaatt
     1141 accgctgatg gtcagggtat tactctggtg aatattggcg tgggaccgtc caatgctaaa
     1201 accatctgcg atcatctggc agtgctacgc ccggatatct ggttgatgat tggtcactgt
     1261 ggcggattac gtgaaagcca ggccattgga gattatgtcc ttgcacacgc ttatttacgc
     1321 gatgaccacg ttcttgatgc ggttctgccg cccgatatac ctattccgag cattgctgaa
     1381 gtgcaacgtg cgctttatga cgctaccaag ttagtcagcg gcaggcctgg tgaggaagtc
     1441 aaacagcggc tgcgtacagg tactgtggta accacagatg acaggaactg ggagttacgt
     1501 tactcagctt ctgcacttcg ttttaactta agccgggccg tagcaattga tatggaaagt
     1561 gcaaccattg ccgcgcaagg atatcgtttc cgcgtgccat acgggacact actgtgtgtt
     1621 tcagataaac cgttacatgg tgagattaag cttcccggcc aggctaaccg tttttatgaa
     1681 ggcgctattt ccgaacacct gcaaattggc attcgggcga tcgatttgct gcgcgcagaa
     1741 ggcgaccgac tgcattcacg taaattacga acctttaatg agccgccgtt ccgataataa
     1801 gttatgaacc ttcttatcag aaatagatgt gaaggattaa gtaagacatc tggcaaatag
     1861 cctgcaataa acgcgggctg cttgcttcct tgggcagatg cgagttttat tatcgtctta
     1921 atgatttcca catattaaaa gcaagtatgc tttcaaaaca caattataaa aaatcccgcc
     1981 aacaacatta gtttttataa aattaaatat aagattatgg ctttagaata tttttatttc
     2041 taatagacga gatttttcct gttatgatat aatatgccga attaacacat gttaacgatt
     2101 taccagtaat gtaaataaat tttcgaggag atcattccag tgggacgtaa atgggccaat
     2161 attgttgcta aaaaaacggc taaagacggt gcaacgtcta aaatttatgc aaaattcggt
     2221 gtagaaatct atgctgctgc caaacaaggt gaacccgatc cagaattaaa cacatcttta
     2281 aaattcgtta ttgaacgtgc aaagcaggca caagttccaa agcacgttat tgataaagca
     2341 attgataaag ccaaaggcgg cggagatgaa acgttcgtac agggacgtta tgaaggcttt
     2401 ggtcctaatg gctcaatgat tattgctgag acattgactt caaatgttaa ccgtacgatt
     2461 gctaacgttc gcacaatttt caataaaaaa ggcggcaata tcggagcggc aggttctgtc
     2521 agctatatgt ttgacaatac gggtgtgatt gtatttaaag gaacagaccc tgaccatatt
     2581 tttgaaattt tgcttgaagc tgaagttgat gttcgtgatg taactgaaga agaaggtaat
     2641 attgttattt atactgaacc tgctgacctt cataaaggaa tcgcggcgct aaaagcagct
     2701 ggaatcactg agttctcaac aacagaatta gaaatgattg ctcaatctga agttgagctt
     2761 tccccagaag atttagaaat ctttgaaggg cttgttgatg cccttgaaga tgacgacgat
     2821 gtacaaaaag tttatcataa cgtcgcaaat ctctaattat attttaaaga aatctgtctt
     2881 tatggcagat ttctttaatc tcatataatt cttataaaaa atataatatt taactcgtca
     2941 tattgcttat aaaaaatccc cgtccccaga gaaataatat ttattaaaat ggcaggtctt
     3001 ctttttctga ttaccgaaga caaagtggca attacgtata gtttcccgat aaagacgcga
     3061 cagctacgtc ccgcataagg catttttctc tttatctttg tacggtactt catggaacag
     3121 aatttttgac cttgtgaatc gtgatatctg ttggggaggg gcaatttgct cactgaagcg
     3181 tgagactcga ttaagcgcac gaaacacaga aatcaaaaaa cccggccact tttttacaaa
     3241 gtggccgggt aaaaataatt tttatttttt aactgtcctg agtctcatag agatgtctca
     3301 aaact
//
LOCUS       DAJTRC010000206         3294 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.212, whole genome shotgun sequence.
ACCESSION   DAJTRC010000206 DAJTRC010000000
VERSION     DAJTRC010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3294
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.212"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..238
                     /locus_tag="OO357_004045"
     CDS             <1..238
                     /locus_tag="OO357_004045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF350 domain-containing protein"
                     /protein_id="HCR5888780.1"
                     /translation="LLGYVIPLSSAAINAVSIPDYFAWGGIALVIQLLVFAGVRLYMP
                     ALSEKIINHNTAAGMFMGTAALAGGIFNAACMTW"
     gene            248..886
                     /locus_tag="OO357_004046"
     CDS             248..886
                     /locus_tag="OO357_004046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1190 domain-containing protein"
                     /protein_id="HCR5888781.1"
                     /translation="MARKRKSRNNSKIGHGAISRIGRPNNPFEPRRNRYAQKYLTLAL
                     MGGAAFFVLKGCSDSSDVDNDGDGTFYATVQDCIDDGNNADICARGWNNAKAAFYADV
                     PKNMTQQNCQSKYENCYYDNVEQSWIPVVSGFLLSRVIRKDRDEPFVYNSGGSSFASR
                     PVWRNTSGDYSWRFGSGKKESYSSGGFTTRKASTVSRGGYGRSSSARGHWGG"
     gene            889..2052
                     /locus_tag="OO357_004047"
     CDS             889..2052
                     /locus_tag="OO357_004047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathionylspermidine synthase family protein"
                     /protein_id="HCR5888782.1"
                     /translation="MLRHNVPVRRDLDQIAANNGFDFHIIDNEIYWDESRAYRFTLRQ
                     IEEQIEKPTAELHQMCLEVVDRAVKDEEILTQLAIPPLYWDVIAESWRARDPSLYGRM
                     DFAWCGNAPVKLLEYNADTPTSLYESAYFQWLWLEDARRSGIIPRDADQYNAIQERLI
                     SRFSELYSRELFYFCCCQDTDEDRSTVLYLQDCAQQAGQESRFIYIEDLGLGVGGVLT
                     DLDDNVIQRAFKLYPLEWMMRDDNGPLLRKRREQWVEPLWKSILSNKGLMPLLWRFFP
                     GHPNLLASWFEGEKSQIAAGESYVRKPIYSREGGNVTIFDGQNNVVDHADGDYADEPM
                     IYQAFQPLPRFGDSYTLIGSWIVDDEACGMGIREDNTLITKDTSRFVPHYIAG"
     gene            2136..>3294
                     /locus_tag="OO357_004048"
     CDS             2136..>3294
                     /locus_tag="OO357_004048"
                     /EC_number="1.3.8.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313190.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HCR5888783.1"
                     /translation="MHWQTHTIFNQPIPLNNSNLYLSDGALCEAVTREGAGWDSDFLA
                     SIGQQLGTAESLELGRLANVNPPELLRYDAQGRRLDDVRFHPAWHLLMQALCTNRVHN
                     LAWEEDARSGAFVARAARFMLHAQVEAGSLCPVTMTFAATPLLLQMLPTPFQDWTTPL
                     LSDRYDSHLLPGGQKRGLLIGMGMTEKQGGSDVMSNTTRAERLEDGSYRLVGHKWFFS
                     VPQSDAHLVLAQTAGGLSCFFVPRFLPDGQRNAIRLERLKDKLGNRSNASCEVEFQDA
                     IGWLLGQEGEGIRLILKMGGMTRFDCALGSHAMMRRAFSLAIYHAHQRHVFGNPLIQQ
                     PLMRHVLSRMALQLEGQTALLFRLARAWDRRADAKEALWARLFTPAAKFVIC"
BASE COUNT          736 a          782 c          939 g          837 t
ORIGIN      
        1 attgttaggt tacgttatcc cattatccag tgcggcaatc aatgcggtga gtattccaga
       61 ctatttcgcc tggggcggga ttgcactggt gattcagtta ctcgtttttg ctggtgtcag
      121 actttatatg cccgcattaa gcgaaaaaat tattaatcac aataccgcag caggaatgtt
      181 tatgggaacc gccgcgctgg ctggcggtat ttttaacgca gcttgtatga catggtaatg
      241 gacgatcatg gccagaaaac gcaaatcaag aaataacagt aaaattggtc acggagcgat
      301 cagtcgcatt ggtagaccga ataatccttt tgaaccgcgt cgcaatcgct acgcacaaaa
      361 atacttaacg ttggcgctaa tgggcggtgc cgcttttttc gtattgaaag gttgtagcga
      421 tagtagcgat gtcgataacg acggcgacgg aacattttac gcgacagtgc aggattgtat
      481 tgatgacggt aataatgccg acatttgcgc gcgtggctgg aacaacgcca aagcggcatt
      541 ttatgccgat gttccaaaga atatgactca gcagaactgt cagtctaagt acgaaaattg
      601 ctactacgac aatgttgaac agagttggat cccggtcgtt tctggatttt tgttaagccg
      661 ggttattcgt aaagatcgcg atgagccgtt cgtctataac agcggcggat cctcatttgc
      721 ttcccgcccc gtctggcgca acacttccgg tgattactcc tggcgcttcg gttctggcaa
      781 aaaagagtct tactcttcag gcggctttac caccagaaaa gcgtcgaccg tttcgcgcgg
      841 cggctatggt cgttcttcca gcgcccgtgg gcattgggga ggctaatcat gctgagacac
      901 aacgttcctg tgcggcggga tctggaccag atcgccgcta acaacggttt cgactttcat
      961 atcatcgaca atgaaatcta ttgggacgag agtcgggctt accgttttac tctgcgccag
     1021 attgaagagc agatcgaaaa accgactgcg gaactgcatc agatgtgcct tgaggtggtg
     1081 gatcgcgcgg ttaaagacga agagatcctg acgcaactgg cgatcccgcc gttgtactgg
     1141 gatgtgatcg ccgaaagctg gcgcgcacgc gatccttcgc tgtatggccg gatggatttt
     1201 gcctggtgtg gcaatgcgcc ggtgaagctg ctggagtaca acgccgatac gccgacctcg
     1261 ttgtatgagt cggcttattt ccagtggttg tggctggagg atgcccggcg cagcggcatt
     1321 attccgcgtg atgccgatca gtacaacgcc attcaggaac gcctgatttc gcgctttagt
     1381 gagctttaca gtcgggaact gttttatttt tgctgctgtc aggacaccga tgaagacagg
     1441 agtaccgtgc tgtacttgca ggactgcgcc cagcaggcag ggcaggagtc gcggtttatc
     1501 tacattgaag atctcggttt gggcgtcggc ggcgtactga ccgatcttga tgataatgtc
     1561 atccagcgtg catttaagct gtatccgctg gagtggatga tgcgtgacga taacggtccg
     1621 ctgctgcgca agcgtcgcga gcaatgggtg gagccgttat ggaaaagtat cttgagtaat
     1681 aaagggctaa tgccgctgct ttggcgcttc ttccctggtc atcctaacct tcttgcatcc
     1741 tggtttgagg gtgaaaaatc gcagattgcc gctggcgaaa gctatgtgcg taaaccgatc
     1801 tactcgcgcg aaggcggaaa cgtcaccatt ttcgacggtc agaataacgt cgttgaccac
     1861 gctgatggtg attacgccga tgaaccgatg atctaccagg cgtttcaacc tctgccgcga
     1921 tttggcgata gctacacact catcggtagc tggattgtcg atgatgaagc gtgcggaatg
     1981 gggatccgtg aagataacac actgatcacc aaagacacct cacgtttcgt tccgcattac
     2041 attgctggat aagaatgttt tagcaatctc tttctgtcat gaatccatgg cagtgaccat
     2101 actaatggtg actgccattg atggagggag acacagtgca ctggcaaact cacaccattt
     2161 ttaatcaacc tataccatta aataacagca acttatacct gtctgatggc gcgctttgcg
     2221 aagcggtaac gcgtgaaggt gctggctggg atagcgattt tctagccagt attggtcagc
     2281 agttaggaac ggctgaatcc cttgaactgg ggcggctggc gaatgtaaac ccgcctgaac
     2341 tgttgcgcta cgatgcccag gggcggcgtc tggacgatgt tcgtttccat cccgcatggc
     2401 acctgctgat gcaggcgtta tgtaccaatc gggtgcacaa tcttgcctgg gaagaagacg
     2461 ctcgctccgg cgcatttgtg gcgcgcgcgg cgcgttttat gttacatgcg caggttgagg
     2521 ctgggtcgtt atgtccagta acgatgacct ttgccgccac gccattgttg ttacagatgt
     2581 tacccacgcc gtttcaggac tggaccacgc cgctgctgag cgatcgctac gattctcact
     2641 tattgccagg tgggcaaaaa cgcggtttgt tgattggcat gggaatgacg gaaaagcagg
     2701 gcggttccga tgtcatgagc aacaccaccc gtgcagagcg tttggaagat ggctcttatc
     2761 ggctggtggg gcataaatgg tttttctcgg tgccgcaaag cgatgcgcat ctggtgctgg
     2821 cgcagaccgc gggcggtctg tcctgctttt ttgtgccgcg ctttttgcct gacgggcaac
     2881 gcaacgcgat tcgcctcgag cggctgaaag ataagctggg taatcgctct aacgccagtt
     2941 gcgaagtgga gtttcaggat gccattggct ggttattggg gcaggaaggg gaaggaattc
     3001 gtctgatcct gaaaatgggg gggatgacgc gttttgattg cgccctgggt agccatgcca
     3061 tgatgcgccg tgcattttcg ctggcgattt atcatgcaca tcaacgccat gtttttggta
     3121 atccattgat ccaacagccc cttatgcgtc atgtcttaag tcgcatggca cttcagcttg
     3181 aagggcaaac ggcgttgctg tttcgtcttg cgcgagcgtg ggaccggcgt gccgatgcca
     3241 aagaagccct gtgggcgcgt ttatttacgc ctgcggcaaa atttgtgatc tgca
//
LOCUS       DAJTRC010000207         3258 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.213, whole genome shotgun sequence.
ACCESSION   DAJTRC010000207 DAJTRC010000000
VERSION     DAJTRC010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3258)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3258)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3258
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.213"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            1..442
                     /locus_tag="OO357_004049"
                     /pseudo
     CDS             1..442
                     /locus_tag="OO357_004049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003031967.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            1026..1742
                     /locus_tag="OO357_004050"
     CDS             1026..1742
                     /locus_tag="OO357_004050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888784.1"
                     /translation="MNDNSLLRNSSLFIAYMGCVGWVSAYSYGWGTSFYYGFPWWVVG
                     AGLDDVARSLLYAIIVMGILFTGWGIGILFFLLIKKRSKIQDLSFFRLFFAITLLFFP
                     VIFELLILKQYFILPLSLSFIISSLVISIIIRIYGRIFSVSCFSDIPFVREHRIKLIM
                     AGFLVYFWFFSFLVGWYKPQLKKEYQMLCYNNSWYYVLARYDSRLVLSSSFKDDSNRF
                     LIFNTEQSGFYEINDVYVRK"
     gene            2294..2698
                     /locus_tag="OO357_004051"
     CDS             2294..2698
                     /locus_tag="OO357_004051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858300.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888785.1"
                     /translation="MNTALIVALMCMWYAVPAAAKETLLAMPRNSTEHCYAEINVHGP
                     YGVYFRVVPHPPGGKSWVECNSDYYYSDKPPGVQILGTRAGCRVYGICGTTSTLHVAG
                     RGVVCIKNICSPRGMIIHRIRKRPVVAVSDEM"
     gene            2761..3258
                     /locus_tag="OO357_004052"
                     /pseudo
     CDS             2761..3258
                     /locus_tag="OO357_004052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          909 a          675 c          749 g          925 t
ORIGIN      
        1 gcgttctaag ccttcccacc atgatcgggg aagccccaga tatgaacagg cacattcaga
       61 gttactttaa agagcttact gaacgtctga ggtgttagag ccatgactgc cgcagcattg
      121 ctggcggaaa tgcctgaatc gagcagcctc tcgagacgtg aaatcagcgc acttgtcggt
      181 gttgctcagg taaaccggga ctcgggaact ctgcgcggtc gacgaaccat tttcggtgat
      241 tgtgctgggg aagaacagct ctgtacatgg cggcgcttga ggcccccccc ggtttaatct
      301 ggtgataaag gcgttttaca tgcgcctact tgcagccgga aacgccaaaa aagtggcact
      361 ggttgcctgt atgcgtaagt tgctgaccat tatgaacgca atgctcagaa aaaacgaaga
      421 atggaatgaa tcgtatttat gaaaactgga ttgtgggcca taaatattcc aaaaagatag
      481 cccgctccgt ttattaccgg aacgggcgag agcatgacat tgtctgttat tataatgaga
      541 ctgctgattc tacttatttt taatttcaag aaaagcgttt tctacaaaga attatgagat
      601 ttaatgtttt gtttagacaa tattatcatt ctgttcaata atgaaagtct tcatcaacaa
      661 caagtaaaaa caacgtccct ctcatcgaac cggacttgca gatttcccgc atccggctct
      721 cttgcaacac atcagacctt cacgttcggg cggttatggg gaaggtccag caagctgtat
      781 gagccctaac tgtttgaata ccagtagatt accagaaact tctatcgatt gaagacatta
      841 ccaactagcg ttactaaatg ttgaccaagt tttgttcact tgtctggttt tgatagtagc
      901 attctgcaga gtattttgat atgataaata tgcattaaca atgatattat tgttaatttc
      961 ctagcatgaa aattaaaaga tcaactttta tctcgaatat tttttacatt atcagttagt
     1021 ttcttatgaa tgacaattca ttgcttagaa attcttcatt attcatcgcc tatatgggat
     1081 gtgtagggtg ggtcagtgct tattcttatg gctggggaac ctctttttac tatggatttc
     1141 catggtgggt tgttggtgca ggtttggatg atgttgccag aagtttactc tatgctatca
     1201 tagtaatggg tatacttttt accggttggg ggattggtat tttatttttt cttcttataa
     1261 agaaaagaag taaaatacaa gacttgagtt tttttagact attttttgct attaccctac
     1321 tgtttttccc tgtaatattc gaacttttaa ttctcaaaca atatttcata cttccattat
     1381 cactttcatt cattattagt tcattggtta tttcaataat aattcgtata tatggacgga
     1441 tcttttctgt atcatgtttt tcagatatcc cttttgttag agaacaccgc attaagttaa
     1501 ttatggctgg ttttttggtt tatttttggt ttttttcctt tcttgttggc tggtataaac
     1561 ctcaattaaa aaaagaatat cagatgcttt gttataataa tagttggtat tatgttcttg
     1621 ctcgttatga ttcacggctc gttttatcta gttcatttaa agatgacagt aacagattct
     1681 taatatttaa tacagaacag tcgggatttt atgaaatcaa tgatgtttat gtcagaaagt
     1741 aataatcctc tcccacaaag tgaattaaat gaatatataa tgcgttcgaa gtatatgtat
     1801 tgagctgtca tactgagaaa caaaccttag ggcagcacaa ttaaagccag aaacaactat
     1861 gcaagaaagt tactaaataa gtgctcggaa cagtaaaaca ctcagggggg actcagtccg
     1921 gattatgtaa tcagataaat tgccaaacat cacaaatcac cagccggact gagtgatgcc
     1981 gagcattgca ccaatacccc gtgatgaacg atgcctgatg cagaaagctc cctataaaac
     2041 aaagaacaaa aactacgacc ggcgattcac cgtcatactg atgctgcatc gtggtgacac
     2101 tgtctgtcac tttcatgttg actgaacaac tctgctgcga agaaggtgta ccgccggcgg
     2161 gatttgattg tccggcgtct agcttccact tccctgatgg aactgaaagt tgttatggcc
     2221 ggtcagcttg ccctgtccgc aggacatgag gtgctgtgac ggacagcacc agtaacggaa
     2281 agtgacaatg aaaatgaata ctgcgctgat agttgcacta atgtgtatgt ggtatgcggt
     2341 acccgctgct gcaaaagaga ctttactggc tatgccacgt aattcaacag aacattgcta
     2401 tgcggagatt aatgtgcacg gaccatatgg ggtgtatttc cgagtggtcc cgcatccccc
     2461 cgggggaaag agctgggtcg aatgtaacag tgactactat tactctgata aaccaccggg
     2521 agtacagata ctcggaaccc gtgcagggtg tcgggtatac ggaatctgcg ggaccacctc
     2581 gacactgcat gtggctggaa gaggggttgt gtgtattaaa aatatatgca gtccacgtgg
     2641 gatgataatt cacaggatac ggaaacgtcc tgtagtggct gtaagcgatg agatgtgatc
     2701 ttacccagca atagtagaca cgcggctaag tgagtaaact ctcagtcaga ggtgactcac
     2761 atgacaaaaa cagtatcaac cagcaaaaaa acacgtaaac agcattcgcc tgaattttgc
     2821 agtgaagccc tgaagcttgc tgaacgcatc ggtgttgctg ccgcagcccg tgaactcagc
     2881 ctgtatgaat ctcagctcta tgcgtggcgc agcaaactgc agcaacagat gacctcgtct
     2941 gagcgtgaaa gtgaactgcc cgcctgaagc ggcaactggc ggagcaggca gaggaactgg
     3001 ctatcctcca aaaggccgcg acatactttg cgcagcggct ggtatacgtg gtgtcagcgt
     3061 cggacaggaa taagccctcg tcagcagttc cgtcagcact gcgacagcgt tgtcctcgcg
     3121 gcggccttta cccggtcaaa acagcgttac ggtgcaccac gcctgacgga tgaactgcgt
     3181 gctcagggtt accactttaa cgtaaaaacc gtggcggcaa gcctgcgccg tcaggggctg
     3241 agggcaaagg cctcccgg
//
LOCUS       DAJTRC010000208         3221 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.214, whole genome shotgun sequence.
ACCESSION   DAJTRC010000208 DAJTRC010000000
VERSION     DAJTRC010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3221)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3221)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3221
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.214"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..121
                     /locus_tag="OO357_004053"
     CDS             <1..121
                     /locus_tag="OO357_004053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HCR5888786.1"
                     /translation="GAALFSLYESKGWLTRHLGYREVTITEKGYAAFKTHFHI"
     gene            complement(99..722)
                     /gene="tetR(B)"
                     /locus_tag="OO357_004054"
     CDS             complement(99..722)
                     /gene="tetR(B)"
                     /locus_tag="OO357_004054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5888787.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            804..2009
                     /gene="tet(B)"
                     /locus_tag="OO357_004055"
     CDS             804..2009
                     /gene="tet(B)"
                     /locus_tag="OO357_004055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5888788.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2122..2715)
                     /gene="tetC"
                     /locus_tag="OO357_004056"
     CDS             complement(2122..2715)
                     /gene="tetC"
                     /locus_tag="OO357_004056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5888789.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2848..3219
                     /locus_tag="OO357_004057"
     CDS             2848..3219
                     /locus_tag="OO357_004057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5888790.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
BASE COUNT          875 a          589 c          658 g         1099 t
ORIGIN      
        1 tggagccgca ttattttcgc tttatgaatc taaagggtgg ttaactcgac atcttggtta
       61 ccgtgaagtt accatcacgg aaaaaggtta tgctgctttt aagacccact ttcacattta
      121 agttgttttt ctaatccgca tatgatcaat tcaaggccga ataagaaggc tggctctgca
      181 ccttggtgat caaataattc gatagcttgt cgtaataatg gcggcatact atcagtagta
      241 ggtgtttccc tttcttcttt agcgacttga tgctcttgat cttccaatac gcaacctaaa
      301 gtaaaatgcc ccacagcgct gagtgcatat aatgcattct ctagtgaaaa accttgttgg
      361 cataaaaagg ctaattgatt ttcgagagtt tcatactgtt tttctgtagg ccgtgtacct
      421 aaatgtactt ttgctccatc gcgatgactt agtaaagcac atctaaaact tttagcgtta
      481 ttacgtaaaa aatcttgcca gctttcccct tctaaagggc aaaagtgagt atggtgccta
      541 tctaacatct caatggctaa ggcgtcgagc aaagcccgct tattttttac atgccaatac
      601 aatgtaggct gctctacacc tagcttctgg gcgagtttac gggttgttaa accttcgatt
      661 ccgacctcat taagcagctc taatgcgctg ttaatcactt tacttttatc taatctagac
      721 atcattaatt cctaattttt gttgacactc tatcattgat agagttattt taccactccc
      781 tatcagtgat agagaaaagt gaaatgaata gttcgacaaa gatcgcattg gtaattacgt
      841 tactcgatgc catggggatt ggccttatca tgccagtctt gccaacgtta ttacgtgaat
      901 ttattgcttc ggaagatatc gctaaccact ttggcgtatt gcttgcactt tatgcgttaa
      961 tgcaggttat ctttgctcct tggcttggaa aaatgtctga ccgatttggt cggcgcccag
     1021 tgctgttgtt gtcattaata ggcgcatcgc tggattactt attgctggct ttttcaagtg
     1081 cgctttggat gctgtattta ggccgtttgc tttcagggat cacaggagct actggggctg
     1141 tcgcggcatc ggtcattgcc gataccacct cagcttctca acgcgtgaag tggttcggtt
     1201 ggttaggggc aagttttggg cttggtttaa tagcggggcc tattattggt ggttttgcag
     1261 gagagatttc accgcatagt ccctttttta tcgctgcgtt gctaaatatt gtcgctttcc
     1321 ttgtggttat gttttggttc cgtgaaacca aaaatacacg tgataataca gataccgaag
     1381 taggggttga gacgcaatcg aattcggtat acatcacttt atttaaaacg atgcccattt
     1441 tgttgattat ttatttttca gcgcaattga taggccaaat tcccgcaacg gtgtgggtgc
     1501 tatttaccga aaatcgtttt ggatggaata gcatgatggt tggcttttca ttagcgggtc
     1561 ttggtctttt acactcagta ttccaagcct ttgtggcagg aagaatagcc actaaatggg
     1621 gcgaaaaaac ggcagtactg ctcggattta ttgcagatag tagtgcattt gcctttttag
     1681 cgtttatatc tgaaggttgg ttagttttcc ctgttttaat tttattggct ggtggtggga
     1741 tcgctttacc tgcattacag ggagtgatgt ctatccaaac aaagagtcat cagcaaggtg
     1801 ctttacaggg attattggtg agccttacca atgcaaccgg tgttattggc ccattactgt
     1861 ttgctgttat ttataatcat tcactaccaa tttgggatgg ctggatttgg attattggtt
     1921 tagcgtttta ctgtattatt atcctgctat cgatgacctt catgttaacc cctcaagctc
     1981 aggggagtaa acaggagaca agtgcttagt tatttcgtca ccaaatgatg ttattccgcg
     2041 aaatataatg accctcttga taacccaaga gggcattttt tacgataaag aagatttagc
     2101 ttcaaataaa acctatctat tttatttatc tttcaagctc aataaaaagc cgcggtaaat
     2161 agcaataaat tggccttttt tatcggcaag ctcttttagg tttttcgcat gtattgcgat
     2221 atgcataaac cagccattga gtaagttttt aagcacatca tcatcataag ctttaagttg
     2281 gttctcttgg atcaatttgc tgacaatggc gtttacctta ccagtaatgt attcaaggct
     2341 aattttttca agttcattcc aaccaatgat aggcatcact tcttggatag ggataaggtt
     2401 tttattatta tcaataatat aatcaagata atgttcaaat atactttcta aggcagacca
     2461 accatttgtt aaatcagttt ttgttgtgat gtaggcatca atcataatta attgctgctt
     2521 ataacaggca ctgagtaatt gttttttatt tttaaagtga tgataaaagg cacctttggt
     2581 caccaacgct tttcccgaga tctcatctat tgaaacagct tgatagcctt tttcaacaaa
     2641 caatattcgt gctgagttaa ccagtgattg ataggtactc ttaaaatttt cttgttgatg
     2701 atttttattt tccatgatag atttaaaata acataccgtc agtatgttta tggtatcatg
     2761 atgatgtggt cgtgacaatc ttaagaacat ttaggttatt ttatgtatat tgaacagcat
     2821 tctcgctatc aaaataaagc taataacatc caattaagat atgatgataa gcagtttcat
     2881 acaacggtta tcaaagatgt tctattatgg attgaacata atttagatca gtctttactg
     2941 cttgatgatg tggcgaataa agcgggttat accaagtggt attttcagcg gctgttcaaa
     3001 aaagtaacag gggtcacact ggctagctat attcgtgctc gtcgtttgac gaaagcggct
     3061 gttgagttga ggttgacgaa aaaaactatc cttgagatcg cattaaaata tcaatttgat
     3121 tcccaacaat cttttacacg tcgatttaag tacattttta aggttacacc aagttattat
     3181 cggcgtaata aattatggga attggaggca atgcactgag a
//
LOCUS       DAJTRC010000209         3181 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.215, whole genome shotgun sequence.
ACCESSION   DAJTRC010000209 DAJTRC010000000
VERSION     DAJTRC010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3181)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3181)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3181
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.215"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            418..1527
                     /gene="fepE"
                     /locus_tag="OO357_004058"
     CDS             418..1527
                     /gene="fepE"
                     /locus_tag="OO357_004058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001421676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS O-antigen length regulator"
                     /protein_id="HCR5888791.1"
                     /translation="MKISKVSDEHFSNYNMLPNGNEEIDLFGLISVLWAAKIKIVGVI
                     LFFACIGFLVSFVLPQKWTSSAVVTPAESVQWSELNKELSKLHVLGVDFDINRDSAFA
                     LFIKKFQSVNSLNEYMRSSPYVMEIIKKNNISALELHRAIVELSKNMKSIDDNASKKN
                     DKSSLYVSWTLSFTAPTSKEAHDVLSGYINYVSSLVVRDLMEDIRNKLEVKTNVEKEI
                     LALDEIKIRNQLNADIRRLNYSLEVANAAGIKKPVYSNGQIMKDDPDFPVALGSDGIA
                     TKLNIKKSIKDVSELSGELRNRQYVVNQLVVAKVGDVDFMPFQYQLSPTLPVRKDGPS
                     NAIIVILSSVIGGMLACGYILLRHAMEARKMSVIE"
     gene            complement(2552..2746)
                     /locus_tag="OO357_004059"
                     /pseudo
     CDS             complement(2552..2746)
                     /locus_tag="OO357_004059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001041627.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rop family plasmid primer RNA-binding protein"
BASE COUNT          801 a          659 c          776 g          945 t
ORIGIN      
        1 aaaaaaaaca ttatcttata ttaggggtgc tgctagcggc gcggtgtgtt tttttacagg
       61 acgcccctgg gggcgctgct aggggtgtct gttcagatat gtagcaatga gtcagggatt
      121 cacgaactga aaatgtccga aaatgtgtct catgggcact gtatgcgccc aaaccgatcg
      181 tcattcactt tcatgcatag ctatgcagtg agctgaaagc gatcctgacg catttttccg
      241 gtttaccccg gggaaaacat ctctttttgc ggtgtttgcg gcagaatcgc gttcagcgcg
      301 ttttagcggt gcgcgcaata aaatgttatg ttaaatgtct gctgaatggt ttttaggttt
      361 tttcctaaca tatgatagaa taaaaaaacc atatgttaat atattgtgtt tttttcaatg
      421 aaaatctcaa aggtctctga cgaacatttt tctaattaca acatgttgcc gaatggtaat
      481 gaagaaattg acctttttgg cttgattagc gttttatggg cagcaaaaat aaaaattgtt
      541 ggggtgattc tattttttgc ttgtattggc tttcttgttt cttttgtttt gccacaaaaa
      601 tggactagtt ctgctgtggt tactcctgct gagtctgttc aatggagtga attaaataag
      661 gagctttcca agcttcatgt tctgggagtg gattttgata tcaatcgtga tagcgcattt
      721 gctctgttta ttaagaaatt tcagtccgtt aattcgttga atgagtatat gagatcatct
      781 ccttatgtta tggagattat taaaaagaat aacattagcg ctctcgaact gcatcgtgca
      841 attgttgagt tgagtaaaaa tatgaagtcg attgatgata atgccagtaa gaaaaacgac
      901 aagtcatcat tgtatgtatc atggactctg agttttactg ctccaacaag taaagaagct
      961 cacgatgtgt tgtctgggta tattaattat gtttcttccc ttgttgtaag ggatttgatg
     1021 gaagatataa gaaataaact agaagttaaa actaatgttg aaaaagaaat tcttgcactg
     1081 gatgagataa aaattagaaa ccagctgaat gcagatattc gacgcctcaa ttattcactg
     1141 gaggttgcta atgcggctgg aataaaaaaa cctgtataca gcaatggtca gattatgaag
     1201 gatgacccag attttcctgt ggctctcggt tctgatggta tagcaactaa attgaacatc
     1261 aaaaaatcaa tcaaggatgt ttcggaattg agtggggagt tgcgaaatcg tcaatatgtt
     1321 gtgaatcaat tggttgtggc gaaagtgggg gatgttgatt ttatgccatt tcagtatcaa
     1381 ttgagcccaa cgttaccggt cagaaaggat gggccaagta atgccataat tgtaattctt
     1441 tcttctgtca ttggagggat gcttgcatgt ggctatatac ttttacgtca tgccatggag
     1501 gccagaaaga tgagtgtgat tgagtaaaag ttgtgctgat gcatcatttt ataagcaact
     1561 tttataaaaa gttcaaagga tcttcttgag atcattccct tacagccgta gtcttctact
     1621 ctagaaaacg aaaaaaccac ctggggaggt ggtttttcga aggttcagta agttggggaa
     1681 cttctgaacc gtggtaacag tcttgtgcga gacatgtcac caaatctgtc cttctagtgt
     1741 agcctcagta aggctgccac ttcaagaact ctcaatacat ctctcgcaca tcctgtttac
     1801 cagtggccgc tgccaatggc gttaagtcgt gtcttaccgg gttggactca agacgatagt
     1861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     1921 agcgaactgc ctacccggaa ctgagtgtca ggcgtggaat gagacgaacg cggccataac
     1981 agcggaatga caccggtaaa ccgaaaggca ggaacaggag agcgcacgag ggagccacca
     2041 gggggaaacg cctggtatct ttaagtcctg tcgggtttcg ccacctctga cttgagcgtc
     2101 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccacc ggcgcggcct
     2161 tttttcgggg cctctttttt gctcacatgt tttttctggt gttatcccct gattctgtgg
     2221 ataaccgtat atccgtcttt gcgtgagcag acaccgctcg ccgcagccga acgaccgagc
     2281 gcagcgagtc agtgagcgag gaagcggaag agcgccggaa gctgcatttt ctccttacgt
     2341 atctgtgcag catttcacac ctcatatgtc gctgttctct caccatctgc tctgccgcct
     2401 cataattaag ccagtataca ctccgttatc gctacgtgac tgggtcatgg ctgcgccccg
     2461 acacccgcta aaaccccctg acgcgccctg acgggcttgt ctgctgacga aaacgcccac
     2521 gggaaagccg tgaccgcctc cgtcagcttc gttatgttcc tgtttcactt tcggcctcaa
     2581 agcgaatctg gatgctgttc tggagttctt ctgcgagttc gtgcagtcgt ttgcacatgg
     2641 ccgcctgttc gtcggcatcc agagcatcca gtttttcaag cagtgtcagg ctctggcttt
     2701 tgatacatcg cgccatattc agcgcagttt gttgttattt gttcaccttc cgttgttctc
     2761 cgtcagttcc gggtgtcggg ggggggggac cctgagcagg tggtaattgt aagacgttgc
     2821 gggcgacggt attacaattg cacatcctgt cctgtttctt taggacgtat catggcacag
     2881 catatcaact gataaataat ccctgtgcag tgcagccgct gttgcataaa atttcgctct
     2941 gcgtcgagtg tttcttaatc aataattccg ctgatactcc ggaaaaacgc gagtctgttc
     3001 tcatgcctga aatgcccaca ccccacgcaa aaacaagttt ttgctgattt ttctttataa
     3061 atagagagtt atgacaaatt agttcttctt gctctctttg tgatatttaa aaaagcggtg
     3121 tcggcgcggt gttgtagctg cgccaacacc gctttttagg ggtggtactg actattttca
     3181 t
//
LOCUS       DAJTRC010000210         3154 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.216, whole genome shotgun sequence.
ACCESSION   DAJTRC010000210 DAJTRC010000000
VERSION     DAJTRC010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3154)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3154)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3154
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.216"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..1169)
                     /locus_tag="OO357_004060"
     CDS             complement(<1..1169)
                     /locus_tag="OO357_004060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HCR5888792.1"
                     /translation="MFSEVMRYILDLGPTVMLPVVIIIFSKILGMKAGDCFKAGLHIG
                     IGFVGIGLVIGLMLDSIGPAAKAMAENFDLNLHVVDVGWPGSSPMTWASQIALVAIPI
                     AILVNVAMLLTRMTRVVNVDIWNIWHMTFTGALLHLATGSWMIGMAGVVIHAAFVYKL
                     GDWFARDTRNFFELEGIAIPHGTSAYMGPIAVLVDAIIEKIPGVNRIKFSADDIQRKF
                     GPFGEPVTVGFVMGLIIGILAGYDVKGVLQLAVKTAAVMLLMPRMIKPIMDGLTPIAK
                     QARSRLQAKFGGQEFLIGLDPALLLGHTAVVSASLIFIPLTILIAVCVPGNQVLPFGD
                     LATIGFFVAMAVAVHRGNLFRTLISGVIIMSITLWIATQTIGLHTQPAANAGALKA"
     gene            complement(1173..1457)
                     /gene="gatB"
                     /locus_tag="OO357_004061"
     CDS             complement(1173..1457)
                     /gene="gatB"
                     /locus_tag="OO357_004061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIB"
                     /protein_id="HCR5888793.1"
                     /translation="MKRKIIVACGGAVATSTMAAEEIKELCQSHNIPVELIQCRVNEI
                     ETYMDGVHLICTTARVDRSFGDIPLVHGMPFVSGVGIEALQNKILTILQG"
     gene            complement(1488..1940)
                     /gene="gatA"
                     /locus_tag="OO357_004062"
     CDS             complement(1488..1940)
                     /gene="gatA"
                     /locus_tag="OO357_004062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIA"
                     /protein_id="HCR5888794.1"
                     /translation="MTNLFVRSGISFVDRSEVLTYIGNEMLAKGVVHDTWPQALIARE
                     AEFPTGIMLEQHAIAIPHCEAIHAKSSAIYLLRPTNKVHFQQADDDNDVAVSLVIALI
                     VENPQQQLKLLRCLFGKLQQPDIVETLITLPETQLKEYFTKYVLDSDE"
     gene            complement(1950..>3154)
                     /gene="gatZ"
                     /locus_tag="OO357_004063"
     CDS             complement(1950..>3154)
                     /gene="gatZ"
                     /locus_tag="OO357_004063"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase subunit GatZ"
                     /protein_id="HCR5888795.1"
                     /translation="SAHPLVIEAALAFDRNSTRKVLIEATSNQVNQFGGYTGMTPADF
                     REFVFTIADKVGFARERIILGGDHLGPNCWQQENADAAMEKSVELVKAYVRAGFSKIH
                     LDASMSCAGDTIPLAPETVAERAAVLCFAAESVATDCQREQLSYVIGTEVPVPGGEAS
                     AIQSVHITHVEDAANTLRTHQKAFIARGLTEALTRVIAIVVQPGVEFDHSNIIHYQPQ
                     EAQALAQWIENTRMVYEAHSTDYQTRTAYWELVRDHFAILKVGPALTFALREAIFALA
                     QIEQELIAPENRSGCLAVIEEVMLDEPQYWKKYYRTGFNDSLLDIRYSLSDRIRYYWP
                     HSRIKNSVETMMVNLQGVDIPLGMISQYLPKQFERIQSGELSAMPHQLIMNKIYDVLR
                     AYRYGCAE"
BASE COUNT          866 a          841 c          685 g          762 t
ORIGIN      
        1 gcttttaacg ccccagcatt agccgccggt tgggtgtgca aaccaatagt ttgcgtcgcg
       61 atccacaggg tgatgctcat aatgatgaca cccgagatta aggtgcggaa cagatttcca
      121 cgatgcaccg cgaccgccat cgccacgaag aagccgatag tggcaagatc gccaaacggc
      181 agtacctgat tacccggaac acaaacagca attaaaatgg tgagcggaat aaaaatcagg
      241 cttgccgata ccaccgccgt atgccccagc agtaatgcgg gatccaggcc aatcaggaac
      301 tcctgaccgc cgaacttcgc ctgtaaacga ctacgggcct gcttagcgat gggcgttaaa
      361 ccatccatga tgggtttaat catccgtggc atcagcagca taactgccgc cgtttttacc
      421 gccagctgca atacaccttt gacatcgtaa cccgcgagga tgccgataat cagccccatc
      481 acaaaaccca cggtgacagg ttcgccaaaa gggccaaatt tgcgctgaat atcgtcggcg
      541 ctaaatttaa ttcggttaac gcctgggatt ttctcgatga tagcatcgac cagcaccgca
      601 atcggcccca tatacgccga cgtaccgtgc ggaatagcaa taccttccag ctcaaagaaa
      661 tttcgggtat cgcgggcaaa ccagtcgccg agcttataaa caaacgccgc gtgaattacc
      721 acacccgcca tccctatcat ccatgaaccg gttgccaggt gcagcaacgc gccggtgaag
      781 gtcatatgcc agatattcca gatatcaaca tttaccaccc gcgtcatacg ggtcagtaac
      841 atcgccacgt taaccaatat cgcaatcgga atcgccacca gcgcaatttg cgacgcccag
      901 gtcattggtg aagagcccgg ccagccgaca tcgaccacat gcagattcag gtcgaaattt
      961 tccgccatcg ctttcgccgc cggaccaatg gaatccagca ttaagccaat cacaaggcca
     1021 atgccaacaa agccaatccc gatatgcaga cccgctttaa agcaatcgcc tgccttcatg
     1081 cctaatattt tagaaaaaat aatgatgaca accggcagca tcactgtagg gccgaggtcg
     1141 agaatataac gcatgacttc tgaaaacata ggtcatccct gtaagatagt cagaatttta
     1201 ttttgtaatg cttcgatacc gacaccagaa acaaaaggca tgccgtgaac taacggaata
     1261 tcgccaaaac tacgatccac cctggcagtg gtacatatca aatgcacacc atccatatag
     1321 gtttctattt cattaacccg acactggatt aattcaacag gaatattatg actctgacac
     1381 aactctttaa tttcttccgc cgccatcgta gaggtcgcaa ccgcgcctcc gcaagcgaca
     1441 ataatcttgc gtttcataaa tagtcctttt tattgttaca gagggattta ttcgtctgaa
     1501 tctaaaacat actttgtgaa gtattccttt aactgagttt caggaagagt aattagtgtc
     1561 tcgacgatat cgggctgttg taacttgcca aataaacagc gtaaaagttt caattgctgc
     1621 tgcggatttt ccacaatcaa cgcaataacc aacgataccg ccacgtcgtt atcatcatcc
     1681 gcttgctgaa aatgaacttt atttgttggc cttaacagat aaatggctga cgacttagca
     1741 tgaatcgcct cacaatgcgg tattgcaatg gcgtgctgct caagcattat cccggtaggg
     1801 aattctgctt ctctggcaat taatgcctgt ggccaggtat catgaaccac acctttcgcg
     1861 agcatctcat taccgatata ggttaaaact tcgctacgat cgacaaaaga aattccgcta
     1921 cgaacaaaca ggttagtcat ataccgtcct tattccgcac agccgtagcg ataggcgcgc
     1981 aaaacatcat aaattttatt cataataagc tgatgcggca ttgctgataa ttccccggac
     2041 tgaatgcgtt caaattgttt gggaagatac tgactaatca tgcccagtgg gatgtccacg
     2101 ccttgcagat tcaccatcat cgtttcaacg ctatttttaa tccgactatg cggccagtaa
     2161 taacgaatac gatccgacag gctgtaacga atatccagta atgaatcgtt aaaacccgtg
     2221 cgataatatt ttttccagta ttgcggttcg tcgagcatca cttcttcaat taccgccagg
     2281 caaccgctgc gattttcggg ggcgataagt tcctgctcaa tttgtgccag tgcaaatatc
     2341 gcctcgcgta aagcaaaggt taatgcggga ccgactttca atattgcaaa gtgatcgcgg
     2401 actaattccc aataagccgt ccgggtctgg taatcggtag aatgtgcttc ataaaccatt
     2461 cgggtgtttt ctatccattg cgccagtgcc tgcgcttcct gcggctgata atggataata
     2521 ttgctgtgat caaattccac acccggctgc accacgatgg caatcacacg tgttaacgcc
     2581 tctgtcagcc cacgggcaat aaaggccttt tgatgcgtac gtaaagtatt ggcggcatct
     2641 tcaacatggg tgatgtgtac tgactgaatg gcgctggcct caccgcccgg aaccggaact
     2701 tcagtgccaa tgacatagct cagttgctca cgctggcaat ctgtcgccac actttccgca
     2761 gcaaagcaaa gcacagcagc tcgttccgca accgtttctg gtgctaacgg tatggtatcc
     2821 cccgcgcagg acattgacgc atcaagatga attttactga agccagcacg aacatatgct
     2881 tttaccagct cgacggattt ttccatcgcc gcatccgcat tttcttgctg ccagcagttt
     2941 ggtcccagat gatcgccgcc gagaataatg cgttcgcgtg caaacccaac tttatcggca
     3001 atcgtaaaaa caaattcgcg aaagtctgcc ggtgtcattc cggtataacc gccaaattga
     3061 ttgacctggt ttgacgttgc ttcaatcagc actttgcgcg tgctgttgcg atcaaatgcc
     3121 agcgccgctt cgataaccaa cggatgggca gaac
//
LOCUS       DAJTRC010000211         3124 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.221, whole genome shotgun sequence.
ACCESSION   DAJTRC010000211 DAJTRC010000000
VERSION     DAJTRC010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3124)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3124)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3124
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.221"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            444..1271
                     /locus_tag="OO357_004064"
     CDS             444..1271
                     /locus_tag="OO357_004064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH/Band 7/PHB domain protein"
                     /protein_id="HCR5888796.1"
                     /translation="MKKVIPALALVLLTTGLVGCDRVEPGNVGIKVNKLGDDKGVGEV
                     VGVGRYWTGWNTEVYIFPTFKQMKTYDEPFSFQMSDGTTIGYHIGVAYKVDPSKVTTV
                     FQTYRKGVDDITDTDLRQKIADALNRLASKMTTDKFIDGGKSELLDAALKDIQEEMTP
                     IGIQVMSLSYVGKPEYPPTVIDSINAKVTANQKTLQCEQEVKQREAEANMLRAEAAGQ
                     ADAIRTKAQAEADAIRLRGEALRQNPNVMELEAINKWNGTLPQYMTSGANTPFIQVK"
     gene            1315..2064
                     /locus_tag="OO357_004065"
     CDS             1315..2064
                     /locus_tag="OO357_004065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ORF6N domain-containing protein"
                     /protein_id="HCR5888797.1"
                     /translation="MNTVTINNKQFPVIEYRGQRVVTLAMIDEVHQRPEGTARAAFNR
                     NREHFISGVDYAELGADVIRTDLPEGTFSKFAPSGIVLFESGYLMLTKPFNDALAWQV
                     QRELVNSYFRTRTPLTEIEMIAAIAADAVRQQKRLNHVEEQLETVTEAVETIKRGNMR
                     AGYVGYRQVVAKSGMSDTKCRNLVNAYQIPTDTHEFMTPDGLLSRRAIVELEPFMAAF
                     HQMMSEADPRGTRWYHPKMGLFQVIGWEDKA"
     gene            2061..2630
                     /locus_tag="OO357_004066"
     CDS             2061..2630
                     /locus_tag="OO357_004066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888798.1"
                     /translation="MIIQSKLIRAALVCAAKNDVRYYLNGLHITPKHIEATNGSVALR
                     MAHGIRTKKNIIVQFEGGVPAKAETTELIFSKEPIAVHRDQFQRRLSITGIKLVDGCF
                     PDLDRIIPKKFDRCTHPVLQAGYLSYPEKMFGRERKFIPVQLRPSGDGQAVRIQFDSI
                     INSMYGNPEFVVMPCRDHGDFNVAQEHPE"
     gene            2754..2996
                     /locus_tag="OO357_004067"
     CDS             2754..2996
                     /locus_tag="OO357_004067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4222 domain-containing protein"
                     /protein_id="HCR5888799.1"
                     /translation="MFALIQRGQIYTDRAGYPVEITRSTEHSVFFRRMDGRTGRVRIG
                     EFSSLFEHIDHLEYHKILAETEQEKHLKKLRAMQRK"
BASE COUNT          874 a          753 c          799 g          698 t
ORIGIN      
        1 gacaagcgtt ttatcttcaa cgggaatgag atatggaaac gtggagaatc aaccacagag
       61 tatacccgcg cacaccagcg aacaatcaga cgactgcgca gactcatcgc caggaaacag
      121 tatacagaaa aatggttcag aaaatatgac acatggagcg ccggatatta cgaactgatg
      181 gcaacaaaac cattctgacg taaacgaaat taaccatgac gcaattaaat aaggcaagcc
      241 gaatacatca ggaggaccat gaacatttat ttcagaatag ttatatcact ggcaattatc
      301 gcatgtattt acggattaat cgttccggcc ctcatatcaa tgaaggatac ggtagcagtg
      361 atttctggct ttgctctggc gtgtctgacc ccgccctgca tttatgccat ttataagggt
      421 ctttcttttt ctaaggataa aagatgaaaa aagttattcc tgctttagcc cttgttctgc
      481 tgaccaccgg acttgtcggt tgcgatcgcg ttgagcctgg taatgttggc atcaaggtaa
      541 acaaactggg cgacgataaa ggcgtcggag aagtggtcgg tgttgggcgc tactggacgg
      601 gatggaacac tgaggtttac atcttcccga ccttcaaaca aatgaagacc tacgatgagc
      661 cattcagctt ccagatgagt gacggtacaa ccatcggcta tcacatcggc gtggcctaca
      721 aagttgatcc atccaaagtt accacagtct ttcagaccta ccgcaaaggc gtggacgaca
      781 ttaccgacac cgacctgcgc cagaagatag ccgacgcact caaccgactg gccagcaaaa
      841 tgaccaccga caaatttatc gacggcggca agtctgagct gctggatgca gctcttaaag
      901 acattcagga agaaatgacg cccatcggta ttcaggtaat gagcctctca tatgtgggta
      961 agccggagta cccgcctact gttatcgaca gcattaatgc caaagtcacg gcgaaccaga
     1021 aaaccctgca atgcgagcag gaagtaaaac agcgcgaagc ggaagccaac atgttgcgcg
     1081 cggaagctgc cggacaggca gatgcgattc gcacaaaagc ccaggccgaa gccgatgcta
     1141 ttcgtttacg cggtgaagct ctgcgccaga acccaaacgt tatggagctg gaagccatca
     1201 acaagtggaa cggtacactg ccgcaataca tgaccagcgg tgccaacaca ccatttattc
     1261 aggttaagta atacatatgc ccggtattac acgccgggct gtctggagat aaaaatgaat
     1321 actgtaacca tcaacaacaa acagtttccg gtaatcgaat atcgcggtca gcgtgttgtg
     1381 acgctggcga tgattgatga agtccaccag cgaccggaag gaaccgcgcg tgcagcattc
     1441 aaccgaaacc gtgagcattt catcagcggt gtggattatg ccgaattagg tgcggacgta
     1501 atacgtacgg acctcccgga aggaacattc tctaaatttg caccatcggg gattgtactt
     1561 tttgaatcag gttacctgat gttgacgaag ccattcaatg acgcccttgc ctggcaggtt
     1621 cagcgcgaac tggttaacag ctacttccgc actcgcacac cgctaacgga aatcgagatg
     1681 atcgctgcga tagccgccga cgctgttcgt caacagaagc gcctgaatca tgtggaagaa
     1741 cagctcgaaa cagtcacaga agcggtggaa accatcaaac gcggaaatat gcgcgccgga
     1801 tatgtcggtt accgccaggt ggtagccaaa agtggaatga gtgacaccaa gtgtcggaat
     1861 ctggtgaacg catatcaaat tcctacagac acccacgaat tcatgactcc ggacggtctg
     1921 ttgtctcgca gggctatcgt cgagcttgag ccatttatgg cggcgttcca ccaaatgatg
     1981 tcagaggctg acccacgcgg cacacgctgg tatcacccga agatggggct tttccaggtg
     2041 atcggatggg aggataaagc atgatcatcc agtcaaaact tattcgcgcc gctctggtgt
     2101 gcgctgctaa aaacgacgtt cgttattacc tgaacggtct tcacatcacg ccgaaacata
     2161 ttgaggcaac caatggttcc gtggcactgc gcatggctca cggcatccgg acgaagaaaa
     2221 acatcattgt ccagttcgaa gggggcgtcc cggccaaagc cgaaacgaca gagctgattt
     2281 ttagtaaaga gccgatcgct gttcatcgcg accagtttca gcgacgactg tccattaccg
     2341 gcattaaact ggtggacggt tgttttccgg atttggatcg catcattccg aaaaagtttg
     2401 accgctgtac tcacccggtg ctccaggcgg gttacctgag ttatccagag aagatgtttg
     2461 gtcgtgagcg taaatttatt cccgtccagt tacgtccctc cggtgacggg caagcggtca
     2521 gaattcagtt tgattccatc atcaactcaa tgtatggcaa tcctgaattt gttgtgatgc
     2581 cttgtcgtga tcatggcgat ttcaacgtgg ctcaggagca tccggaatga aaatcgaata
     2641 ccaggacgcc gcaggaggtg aatcaatgag ctggcctgat gcaatcgtaa ctctggggat
     2701 ggtattcgca gtagcgtttg ttgtgtactc gatttgtcga tggggataac cacatgttcg
     2761 ctttgattca acgcgggcag atatacacag acagagccgg atacccagta gaaattactc
     2821 gcagtactga gcactcagtg ttctttcgga ggatggacgg acgtaccggg cgagtacgca
     2881 tcggtgaatt cagtagcctg ttcgagcata ttgaccacct ggaatatcac aagattctgg
     2941 ctgaaacaga acaagaaaag cacctgaaga aattacgcgc aatgcaaagg aagtaaaaaa
     3001 tgaataaagc gtttgagcta tgggtccacc agcgttacgg caaccactac gacctgacgc
     3061 gagatgttga cggtttctac tgtcgtgaaa ttgtgaaacg aatgtttgag gtctggtgcc
     3121 actg
//
LOCUS       DAJTRC010000212         3112 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.222, whole genome shotgun sequence.
ACCESSION   DAJTRC010000212 DAJTRC010000000
VERSION     DAJTRC010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.222"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO357_004068"
     CDS             complement(<1..709)
                     /gene="ipaH9.8"
                     /locus_tag="OO357_004068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858359.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH9.8"
                     /protein_id="HCR5888800.1"
                     /translation="MLPINNNFSLPQNSFYNTISGTYADYFSAWDKWEKQALPGEERD
                     EAVSRLKECLINNSDELRLDRLNLSSLPDNLPAQITLLNVSYNQLTNLPELPVTLKKL
                     YSASNKLSELPVLPPALESLQVQHNELENLPALPDSLLTMNISYNEIVSLPSLPQALK
                     NLRATRNFLTELPAFSEGNNPVVREYFFDRNQISHIPESILNLRNECSIHISDNPLSS
                     HALQALQRLTSSPDYHGP"
     gene            complement(971..1066)
                     /locus_tag="OO357_004069"
                     /pseudo
     CDS             complement(971..1066)
                     /locus_tag="OO357_004069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
     gene            complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO357_004070"
     CDS             complement(1102..1407)
                     /gene="ccdB"
                     /locus_tag="OO357_004070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858358.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system toxin CcdB"
                     /protein_id="HCR5888801.1"
                     /translation="MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSD
                     KVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEVADLSHRENDIKNAINLMFWGI"
     gene            complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO357_004071"
     CDS             complement(1409..1627)
                     /gene="ccdA"
                     /locus_tag="OO357_004071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin CcdA"
                     /protein_id="HCR5888802.1"
                     /translation="MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERW
                     QAENQQGMAEIARFIEMNGSFADENRDW"
     gene            complement(2163..3112)
                     /locus_tag="OO357_004072"
                     /pseudo
     CDS             complement(2163..3112)
                     /locus_tag="OO357_004072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001546148.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
BASE COUNT          773 a          753 c          757 g          829 t
ORIGIN      
        1 gtgggccgtg gtagtccggc gaagaggtta atctttgcag ggcttgcaga gcatgggatg
       61 ataatgggtt atcactaata tgtattgaac attcattcct cagattaaga atgctttccg
      121 ggatatgact tatctgattt ctatcaaaaa aatactctct gacaacggga ttatttccct
      181 cagaaaatgc tggtagctca gtgaggaaat tacgggtcgc tctgagattt ttaagagcct
      241 gtgggagcga tggtaaggag actatttcgt tatagctgat attcatagtc aataacgaat
      301 cgggtaaagc tggcaggttt tccagctcat tgtgttgtac ctgaagtgac tccagcgcag
      361 gaggtagcac gggcaattct gataatttat tgctggcgga atataatttt tttagcgtaa
      421 caggcagttc aggtaggtta gttaattgat tatatgatac attgagcagc gttatctgag
      481 ctggtaagtt gtcaggtagc gaggacagat ttaaacggtc cagtcgaagt tcatcggaat
      541 tattgataag acattcttta agtcgggaga cagcctcatc acgctcttca ccggggagcg
      601 cttgtttttc ccatttatcc catgctgaaa agtaatcagc atatgtaccg gaaatagtgt
      661 tataaaaaga attttggggc aatgaaaagt tattatttat cggtaacatg atgggcatcc
      721 agttaaagcc ggtggacaaa tggctttgtt aaaagaaata agtaggagtt tttatgaagt
      781 tcattccatg aaatgaactt cagcactttt ttattataat aaggataaac aaaattggct
      841 gtaaaaaaac gatactgttt cagttaaccg tgaatggcct gtttgcaccg tattgttctt
      901 gtttttcggt tagttaaaca tcgtacttca tatttgaacg gtaagcggct cgtcagaacc
      961 gtattgatat ttactgagag gtcagatcaa ctttccaagg caacagatcg cgtacccggt
     1021 ttgccggcca gtcctggata tgctcaatga cgtaacgcag ccattttgtc aacgccacga
     1081 tttttgaccc tagcctgaca tttatattcc ccagaacatc aggttaatgg cgtttttgat
     1141 gtcattttcg cggtggctga gatcagccac ttcttccccg ataacgaaga tcggcacact
     1201 ggccatatca gtggtcatca tgcgccagct ttcatccccg acatgcacca ccgggtaaag
     1261 ctcacgggag actttatctg acagcaggcg ggcactggcc agggggatca ccatccgtcg
     1321 tccgggcgtg tcaataatgt cactctgtac atccacaaac agacgataac gactctctct
     1381 tttataggcg taaaccttaa actgcatttc accagtccct gttctcatcg gcaaaagagc
     1441 cgttcatttc aataaaacgg gcgatctcag ccatcccctg ctgattttca gcctgccagc
     1501 gttccgcacg cagacggcgg gcctcttttt gcatggccgt atttaccagg cctgagatat
     1561 tcacgtttgc ggatttaagt aactgatagc tgtcactgtc tatagtgaca gtaatacgct
     1621 gcttcatgat aagccctcaa aaacatactt atgatatgta tatgagtata tattaatggt
     1681 acaaaccatc aatatccata aaaagtacac cattaattcg ctataagttt ttaatacaat
     1741 tttttctgct gcgatttttt tatactataa ttagcatact tataacaccg taagtaaatt
     1801 acatgaaagc aggcacagcg tgatccgatt tttctgcgta taacgatttt ccggaagtgt
     1861 aatacggttg cgccccgggc cgggcaccct tttctgttgt tgtgtacggg cccacagaga
     1921 atggtaccgg aggcgggctc agaagtactg agtcgggtga tcgcgtcagg ccgcgaagta
     1981 ctgacacggg tattggtgac tggtgatgaa gtactgcccc gggtatgatt tcaggctgat
     2041 tcaggttctg attctctgtc agtccgcgtg gttgctggcc tggctgattt cctgttgtca
     2101 ggcgttcaac aaccgactgc aggttatagc ccggttcacg aacacctgac ggcaggtatc
     2161 actcacccgg tgaaggtaaa tccctcaaga ggcagcaact ctgccagtcg ctcctccggc
     2221 cattccggca gacgcgtcag gacgtccgtc agccaggcat gtggctccag accattacgt
     2281 tttgctgttt ccagaaggct cattatctgt gccgcccggt ttccggccat cagtgaaccg
     2341 gcgaacagcc acgctttccg tcccataaca accgggcgaa tggcacgctc gcagatattg
     2401 ttgtccaggg gcacagctcc gtcacccagg aagcagctca gttcatcgcg ccggttcaga
     2461 atatagttaa tcgccttatt taatggcccg tcgggcggac agcacggctc ctgctcttca
     2521 agccaggcaa acaggtcatt cactatcggc cgggagtacc gttatcgcca ctgccgtatt
     2581 ttttccacag ggcgctcacg gatgagtttc tcaatgcggt aaagacccgc gatttttctc
     2641 agctccacgc ctgcacgggg ctccctgctg gccttataca gatttgcgaa gccgcgccct
     2701 gcgtgactcc agcaacaggc tgatgtgatg ccggggtgat tatccgccag agatttgtac
     2761 acgctgtatc cgtcactcac cagcgtactg ccacaccagc actgcagcca ggtctcaggg
     2821 tatctgaggg cccgtccggt ctgcgagtca aaacagacga ccggcggacc gcttctttca
     2881 ccgctgacgt aagcacacag ccagccggag caggatttac cgcctttccg ggtatcaagg
     2941 agcctcaggg acgtttcatc tgcatgaata acatcccgtg tcagaagctc atggcgtagc
     3001 agtttcgcca gcggggccag tgcagctccg gcaacaccga ccatatccgc cattgtactg
     3061 acggggagct ccacacccat tcgggagaaa atgtgctgct ggcgatacag gg
//
LOCUS       DAJTRC010000213         3107 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.223, whole genome shotgun sequence.
ACCESSION   DAJTRC010000213 DAJTRC010000000
VERSION     DAJTRC010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.223"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            2..2905
                     /locus_tag="OO357_004073"
     rRNA            2..2905
                     /locus_tag="OO357_004073"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            3001..>3107
                     /gene="rrf"
                     /locus_tag="OO357_004074"
     rRNA            3001..>3107
                     /gene="rrf"
                     /locus_tag="OO357_004074"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          820 a          684 c          973 g          630 t
ORIGIN      
        1 aggttaagcg actaagcgta cacggtggat gccctggcag tcagaggcga tgaaggacgt
       61 gctaatctgc gataagcgtc ggtaaggtga tatgaaccgt tataaccggc gatttccgaa
      121 tggggaaacc cagtgtgatt cgtcacacta tcattaactg aatccatagg ttaatgaggc
      181 gaaccggggg aactgaaaca tctaagtacc ccgaggaaaa gaaatcaacc gagattcccc
      241 cagtagcggc gagcgaacgg ggagcagccc agagcctgaa tcagtgtgtg tgttagtgga
      301 agcgtctgga aaggcgtgcg atacagggtg acagccccgt acacaaaaat gcacatactg
      361 tgagctcgat gagtagggcg ggacacgtgg tatcctgtct gaatatgggg ggaccatcct
      421 ccaaggctaa atactcctga ctgaccgata gtgaaccagt accgtgaggg aaaggcgaaa
      481 agaaccccgg cgaggggagt gaaaaagaac ctgaaaccgt gtacgtacaa gcagtgggag
      541 cacgcttagg cgtgtgactg cgtacctttt gtataatggg tcagcgactt atattctgta
      601 gcaaggttaa ccgaataggg gagccgaagg gaaaccgagt cttaactggg cgttaagttg
      661 cagggtatag acccgaaacc cggtgatcta gccatgggca ggttgaaggt tgggtaacac
      721 taactggagg accgaaccga ctaatgttga aaaattagcg gatgacttgt ggctgggggt
      781 gaaaggccaa tcaaaccggg agatagctgg ttctccccga aagctattta ggtagcgcct
      841 cgtgaattca tctccggggg tagagcactg tttcggcaag ggggtcatcc cgacttacca
      901 acccgatgca aactgcgaat accggagaat gttatcacgg gagacacacg gcgggtgcta
      961 acgtccgtcg tgaagaggga aacaacccag accgccagct aaggtcccaa agtcatggtt
     1021 aagtgggaaa cgatgtggga aggcccagac agccaggatg ttggcttaga agcagccatc
     1081 atttaaagaa agcgtaatag ctcactggtc gagtcggcct gcgcggaaga tgtaacgggg
     1141 ctaaaccatg caccgaagct gcggcagcga cgcttatgcg ttgttgggta ggggagcgtt
     1201 ctgtaagcct gtgaaggtgt gctgtgaggt atgctggagg tatcagaagt gcgaatgctg
     1261 acataagtaa cgataaagcg ggtgaaaagc ccgctcgccg gaagaccaag ggttcctgtc
     1321 caacgttaat cggggcaggg tgagtcgacc cctaaggcga ggccgaaagg cgtagtcgat
     1381 gggaaacagg ttaatattcc tgtacttggt gttactgcga aggggggacg gagaaggcta
     1441 tgttggccgg gcgacggttg tcccggttta agcgtgtagg ctggttttcc aggcaaatcc
     1501 ggaaaatcaa ggccgaggcg tgatgacgag gcactacggt gctgaagcaa caaatgccct
     1561 gcttccagga aaagcctcta agcatcaggt aacatcaaat cgtaccccaa accgacacag
     1621 gtggtcaggt agagaatacc aaggcgcttg agagaactcg ggtgaaggaa ctaggcaaaa
     1681 tggtgccgta acttcgggag aaggcacgct gatacgtagg tgaagcgact tgctcgtgga
     1741 gctgaaatca gtcgaagata ccagctggct gcaactgttt attaaaaaca cagcactgtg
     1801 caaacacgaa agtggacgta tacggtgtga cgcctgcccg gtgccggaag gttaattgat
     1861 ggggttagcg caagcgaagc tcttgatcga agccccggta aacggcggcc gtaactataa
     1921 cggtcctaag gtagcgaaat tccttgtcgg gtaagttccg acctgcacga atggcgtaat
     1981 gatggccagg ctgtctccac ccgagactca gtgaaattga actcgctgtg aagatgcagt
     2041 gtacccgcgg caagacggaa agaccccgtg aacctttact atagcttgac actgaacatt
     2101 gagccttgat gtgtaggata ggtgggaggc tttgaagtgt ggacgccagt ctgcatggag
     2161 ccgaccttga aataccaccc tttaatgttt gatgttctaa cgtggacccg tgatccgggt
     2221 tgcggacagt gtctggtggg tagtttgact ggggcggtct cctcctaaag agtaacggag
     2281 gagcacgaag gttggctaat cctggtcgga catcaggagg ttagtgcaat ggcataagcc
     2341 agcttgactg cgagcgtgac ggcgcgagca ggtgcgaaag caggtcatag tgatccggtg
     2401 gttctgaatg gaagggccat cgctcaacgg ataaaaggta ctccggggat aacaggctga
     2461 taccgcccaa gagttcatat cgacggcggt gtttggcacc tcgatgtcgg ctcatcacat
     2521 cctggggctg aagtaggtcc caagggtatg gctgttcgcc atttaaagtg gtacgcgagc
     2581 tgggtttaga acgtcgtgag acagttcggt ccctatctgc cgtgggcgct ggagaactga
     2641 ggggggctgc tcctagtacg agaggaccgg agtggacgca tcactggtgt tcgggttgtc
     2701 atgccaatgg cactgcccgg tagctaaatg cggaagagat aagtgctgaa agcatctaag
     2761 cacgaaactt gccccgagat gagttctccc tgacccttta agggtcctga aggaacgttg
     2821 aagacgacga cgttgatagg ccgggtgtgt aagcgcagcg atgcgttgag ctaaccggta
     2881 ctaatgaacc gtgaggctta accttacaac gccgaagatg ttttggcgga ttgagagaag
     2941 attttcagcc tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaattag
     3001 cctggcggcc gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc
     3061 cgtagcgccg atggtagtgt ggggtctccc catgcgagag tagggaa
//
LOCUS       DAJTRC010000214         3097 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.224, whole genome shotgun sequence.
ACCESSION   DAJTRC010000214 DAJTRC010000000
VERSION     DAJTRC010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3097)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.224"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(104..1813)
                     /locus_tag="OO357_004075"
     CDS             complement(104..1813)
                     /locus_tag="OO357_004075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ShET2/EspL2 family type III secretion system
                     effector toxin"
                     /protein_id="HCR5888803.1"
                     /translation="MPLNKTFSSSIFSTKNSLSTDMSVNRDNRTITSSIMRVSNSSEL
                     IQFKNKTAPYFSEKRNVEVNINGVAKDIYGRQIVCRHLASYWEMNFMETNGKVNYQLL
                     STPDAIAKNVCLEKTEDFSKSPAYIYFVENKKWGTVITNFFYNMKKNGDFVRTLSACT
                     LNHQMALGLKIKRVQESEKWVVQFFDPNRTVTHKRTVFTCDSHFELSQLSAKDFFDDF
                     YWKIYGLEQPGQVIFEDRHNSPLTNTVKLLPDELINSRVIYHAITKNLTEVLFILMEK
                     YKNGEISQSKLVNLLATRSSDGTPAFYIALQNGYSDIIQVYGKILNMCNLSQETILTL
                     LAAVGANNVPGLCMSFMNGHVDTIKAYGEIVFKTPLTSDKRLYLLAAKDSHDLPGLFF
                     ALQNGHADSIRMFGSLLNKKMLSSEQIKELLKVKHGLFMALQNGHTKAIMAYGDILKI
                     LPPHQEYIDELLWIKNPNGTSGLFMAFYNGHTETIRAFCNILKNYSFTTRRLVEMLSA
                     TNKDGIPGVFVSVVNRDKETILEYCRIIKENNLEPDTIAEQFSKKMKKTFIEIINRFN
                     HFL"
     gene            complement(2245..2964)
                     /locus_tag="OO357_004076"
     CDS             complement(2245..2964)
                     /locus_tag="OO357_004076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector
                     phosphothreonine lyase"
                     /protein_id="HCR5888804.1"
                     /translation="MPIKKPCLKLNLDSLNVVRSEIPQMLSANERLKNNFNILYNQIR
                     QYPAYYFKVASNVPTYSDICQSFSVMYQGFQIVNHSGDVFIHACRENPQSKGDFVGDK
                     FHISIAREQVPLAFQILSGLLFSEDSPIDKWKITDMNRVSQQSRVGIGAQFTLYVKSD
                     QECSQYSALLLHKIRQFIMCLESNLLRSKIAPGEYPASDVRPEDWKYVSYRNELRSDR
                     DGSERQEQMLREEPFYRLMIE"
BASE COUNT          953 a          588 c          519 g         1037 t
ORIGIN      
        1 gccaattgag gtggttgcct gaaagttggc agttacccgg tcactacacc ttgttggagt
       61 gaacatatca aaaacaggta attacacaat ttaaattaca gtctcataaa aaatgattaa
      121 atctatttat tatttcaata aacgtttttt tcatcttctt tgaaaattgt tctgctatag
      181 tatccggttc cagattgttc tcttttatta ttctacaata ctctaatatt gtttctttgt
      241 ctctatttac tacagataca aatacaccag gaataccatc tttattagtt gcggacagca
      301 tttcgaccaa tcgtcttgtg gtaaaactat agttttttag aatattacaa aaggcacgta
      361 ttgtttcggt atggccatta taaaatgcca taaaaagtcc tgatgtaccg ttgggatttt
      421 ttatccataa taattcatct atatattcct ggtgaggggg gagtattttt agtatatctc
      481 catacgccat aatggccttt gtgtgcccat tttgaagtgc cataaacaac ccatgcttta
      541 ctttcaataa ttctttaatt tgttcactac taagcatttt cttattaagc agacttccga
      601 acatacgaat actatctgca tgtccattct gcaatgcaaa aaataaccca ggtaaatcat
      661 ggctgtcttt cgcagctaat agatataatc tcttatcgga agttaacggg gttttaaaga
      721 ctatttcccc ataggctttt attgtgtcta catgtccgtt cataaaagac atgcataaac
      781 ccggcacatt gtttgcccct accgcagcaa gcaatgtaag tattgtttct tgactaagat
      841 tgcacatatt taatatctta ccatatactt gaataatgtc tgaatatccg ttttgaagtg
      901 ctatataaaa tgcaggagta ccatcagagc ttctcgtggc cagaaggttc accagtttag
      961 attgagaaat ttcaccattt ttgtattttt ccatcagtat aaataatact tctgtcagat
     1021 tctttgttat cgcatgatat ataactctgg aattgatcaa ttcatctggt aaaagtttca
     1081 ctgtgtttgt tagaggggaa ttatgcctat cttcaaatat tacctgcccg ggttgctcca
     1141 gaccataaat tttccaataa aaatcatcaa aaaaatcctt agcggataat tgactcagtt
     1201 cgaaatggct atcacaagta aatacagtac gtttatgagt aacagttcga ttaggatcga
     1261 aaaactgaac aacccatttt tcactttctt gtacgcgctt tattttcaac cctaacgcca
     1321 tttggtggtt taatgtgcat gctgataatg ttctgacaaa gtctccattt tttttcatgt
     1381 tataaaaaaa attagtaata actgttcccc actttttatt ttccacaaaa taaatataag
     1441 ctggtgattt tgaaaaatct tccgtttttt ccaaacatac attttttgca atagcatcag
     1501 gggtgctaag aagctgatag tttaccttcc catttgtttc cataaaattc atttcccaat
     1561 aggaagctag gtgacggcaa actatttgtc ttccatatat atcttttgcc actccattta
     1621 tatttacctc cacatttctc ttttctgaaa aataaggtgc tgttttattc ttaaactgaa
     1681 ttagttcact ggagtttgag accctcataa tagaacttgt tattgttcta ttatctctgt
     1741 taacagacat atctgttgat aacgaatttt ttgttgaaaa tatactagat gaaaatgttt
     1801 tatttaacgg catagaagac aaccatagag atgttataat actattagat tgcagttact
     1861 aatagtaaaa gcctccttac aaggaggtag cttattcaga atgctcctat aagatagcga
     1921 gctcgtcaac gatacagatg atctgattat attgtgcaaa aggagaacag gaaaataggc
     1981 actggaaaag atgaaggcct gatgggagca ttaactaatg caaaatccga caggttaata
     2041 tcccgaaaag caatcaaacg actcaaacag gagatgcacg ctgaaatggt ggacaactgt
     2101 tcggatttca gatgagtagc tcagcagttt atgagccgaa tgctctgtca cgggcaggaa
     2161 tcaggctgca tacgcagtgc caaatctaat cgtagttatt ctaaccagtt ttcaagctac
     2221 aaggtggtgt agctggcatc ttctctactc tatcatcaaa cgataaaatg gttcctctcg
     2281 taacatttgc tcttgccttt cacttccatc tcgatcgctt cgtagttcat tacgatagct
     2341 gacatatttc cagtcttcag gtctaacatc tgacgccgga tactccccgg gagcgatttt
     2401 gcttcttaat agattagact cgagacacat tataaattgt cgtattttat gaagtaataa
     2461 agcactatat tgcgagcatt cctgatctga ttttacatat agcgtaaact gagctcctat
     2521 ccccacacga gattgctgag aaactcgatt catatcagtt atcttccatt tatctatagg
     2581 actgtcctct gaaaataata aaccagacaa aatttggaat gccagaggaa cttgctccct
     2641 agctatacta atatgaaact tgtccccaac aaaatccccc ttactctgag gattttcacg
     2701 acatgcatga ataaacacat ctccactatg gttcacaatc tggaatcctt ggtacatgac
     2761 agaaaaagat tgacatatat cagagtaagt tggcacgttc gatgctactt tgaaatagta
     2821 cgctgggtat tgccgtattt ggttgtacaa tatattgaag ttatttttca atctttcatt
     2881 cgcagaaagc atctgcggta tttcagacct cacaacattc aaagaatcta aatttagttt
     2941 tagacagggc ttttttatgg gcatagaaaa cgtcctctat aaaatagata tattatctta
     3001 tattgattgt ttatcatctt ttataaaacg atacattata tgtaatgtat gtaagcgtaa
     3061 gggtcctctc cgaacatggt gggaaggctt agaacgc
//
LOCUS       DAJTRC010000215         3019 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.225, whole genome shotgun sequence.
ACCESSION   DAJTRC010000215 DAJTRC010000000
VERSION     DAJTRC010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.225"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..199
                     /locus_tag="OO357_004077"
     CDS             <1..199
                     /locus_tag="OO357_004077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5888805.1"
                     /translation="AIDGHGLAYLPYDQVERAIKEKKLIRVLDKFTPDLPGYHLYYPH
                     RRHAGSAFSLFIDRLKYKGAV"
     gene            536..2149
                     /gene="mppA"
                     /locus_tag="OO357_004078"
     CDS             536..2149
                     /gene="mppA"
                     /locus_tag="OO357_004078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="murein tripeptide ABC transporter
                     substrate-binding protein MppA"
                     /protein_id="HCR5888806.1"
                     /translation="MKHSVSVTCCALLVSSISLSYAAEVPSGTVLAEKQELVRHIKDE
                     PASLDPAKAVGLPEIQVIRDLFEGLVNQNEKGEIVPGVATQWKSNDNRIWTFTLRDNA
                     KWADGTPVTAQDFVYSWQRLVDPKTLSPFAWFAALAGINNAQAIIDGKATPDQLGVTA
                     VDAHTLKIQLDKPLPWFVNLTANFAFFPVQKANVESGKEWTKPGNLIGNGAYVLKERV
                     VNEKLVVVPNTHYWDNAKTVLQKVTFLPINQESAATKRYLAGDIDITESFPKNMYQKL
                     LKDIPGQVYTPPQLGTYYYAFNTQKGPTADQRVRLALSMTIDRRLMTEKVLGTGEKPA
                     WHFIPDVTAGFTPEPSPFEQMSQEELNAQAKTLLSAAGYGPQKPLKLTLLYNTSENHQ
                     KIAIAVASMWKKNLGVDVKLQNQEWKTYIDSRNTGNFDVIRASWVGDYNEPSTFLTLL
                     TSMHSGNISRFNNPAYDKVLAQASTENTVKARNADYNAAEKILMEQAPIAPIYQYTNG
                     RLIKPWLKGYPINNPEDVAYSRTMYIVKH"
     gene            complement(2200..>3019)
                     /gene="ynaI"
                     /locus_tag="OO357_004079"
     CDS             complement(2200..>3019)
                     /gene="ynaI"
                     /locus_tag="OO357_004079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low conductance mechanosensitive channel YnaI"
                     /protein_id="HCR5888807.1"
                     /translation="FITPGVIDFICTSLIAVILTIKLFLLINQFEKQQIKKGRDITSA
                     RIMSRIIKITIIVVLVLLYGEHFGMSLSGLLTFGGIGGLAVGMAGKDILSNFFSGIML
                     YFDRPFSIGDWIRSPDRNIEGTVAEIGWRITKITTFDNRPLYVPNSLFSSISVENPGR
                     MTNRRITTTIGLRYEDAAKVGVIVEAVREMLKNHPAIDQRQTLLVYFNQFADSSLNIM
                     VYCFTKTTVWAEWLAAQQDVYLKIIDIVQSHGADFAFPSQTLYMDNITPPEQGR"
BASE COUNT          852 a          664 c          707 g          796 t
ORIGIN      
        1 tgcaattgat gggcatggat tggcgtatct accttatgat caggttgagc gggctattaa
       61 agaaaaaaaa ctgatacgtg ttttggataa attcactcca gatttacccg gttatcacct
      121 gtactatcca caccgtcgac atgctggctc ggcattctca ttatttatag ataggctgaa
      181 gtataaaggt gctgtttagc actacttgct gatacattaa tttaattctt ctcttaacgt
      241 attctcagtt cctttcaacg ttttggttat tttttattct tcgtacaatg gcgacagatg
      301 ctgattatga taccgaaaac tggtttgaac gtgcgaagcc cgaacgtagt gttcggtgtt
      361 ctatgtgctt tgccacattt tggcgtctat tatttacact aaatctgatt tgatatattg
      421 atacttaaaa catttgatgc ttcctttgtc acttttttga tggaagttgt ttgcatttct
      481 ttaaggcgaa acaaataatt acgcatcaat tttaatgtcg gttagaggga aacttatgaa
      541 gcactctgtt tcagttacgt gttgtgcgct gttggtcagc agcatttctc tttcgtatgc
      601 tgcagaagtt ccgagcggca cagtactggc agagaagcag gagctggtgc gccacattaa
      661 agatgagcct gcgtcgctgg atcccgctaa agccgtgggc ctgccagaga ttcaggtcat
      721 tcgcgatctg tttgaaggtc tggtgaatca gaacgaaaaa ggggagattg tccccggcgt
      781 cgcgactcaa tggaaaagta atgacaaccg tatctggact tttaccctgc gcgataacgc
      841 aaaatgggcg gatggcacac cggtaacggc gcaagatttt gtctacagct ggcaacgtct
      901 ggtggaccca aaaacattgt cgccatttgc atggtttgcc gcgctggcgg gaatcaacaa
      961 cgcacaggcg attattgatg gtaaagctac gcctgatcag cttggcgtca ccgcagttga
     1021 tgcccatact ttgaaaattc agcttgataa accgttgccg tggtttgtga atttaaccgc
     1081 taactttgcc ttcttcccgg tgcaaaaagc caacgtagaa agcggtaaag agtggacgaa
     1141 acccggaaat ctgatcggca atggcgctta tgttcttaaa gagcgcgtag tcaatgaaaa
     1201 actggtcgtg gtaccgaata cccattattg ggataacgcc aaaacggtgc tgcaaaaagt
     1261 gaccttcctg ccaattaatc aggaatccgc agccactaag cgttacctcg cgggggatat
     1321 tgatatcacc gaatccttcc cgaaaaatat gtatcagaag ctgttgaagg atattccggg
     1381 gcaggtttat acgccaccgc agctcgggac ctattattat gcctttaaca cgcaaaaagg
     1441 gccgacggca gatcagcgcg ttcgcctggc attaagtatg acgatagatc gccgcctgat
     1501 gaccgaaaaa gtgttgggga cgggcgaaaa gccagcgtgg cattttatac cagatgttac
     1561 cgccggattt acgccggaac cttcgccgtt tgaacaaatg agtcaggaag aactgaatgc
     1621 gcaggcaaaa actttgttga gcgcagctgg ttatggtccg caaaaaccgc tgaagctgac
     1681 gcttttgtat aacacttcag aaaaccatca aaaaattgcg attgctgtag catcgatgtg
     1741 gaaaaagaac cttggcgtag atgttaaatt gcaaaatcag gaatggaaaa cctatatcga
     1801 tagccgtaac accggcaatt ttgatgttat ccgcgcctca tgggtggggg attataatga
     1861 accctccact ttcctgacat tattaacgtc aatgcattca ggaaatattt cacgctttaa
     1921 caatccggca tacgacaaag ttctggccca ggcatcgacg gaaaataccg ttaaagcgcg
     1981 taatgccgat tacaacgcgg cagaaaaaat cctcatggag caagcaccga ttgcaccaat
     2041 ttatcaatat accaatggac gattaatcaa accgtggctg aaaggttatc ccattaataa
     2101 ccctgaagat gtagcgtaca gtcggactat gtatattgtg aagcattgat gtgatgggaa
     2161 ctggcgttac ccttgtggat aacgccagtg atgtctgatt tagcgcccct gttccggcgg
     2221 tgtaatgtta tccatataca gcgtctggct ggggaaggca aagtccgcgc cgtgtgactg
     2281 tacaatatcg ataatcttca aataaacgtc ttgctgtgca gcaagccact cagcccatac
     2341 cgtggttttg gtaaagcaat aaaccataat attcaatgac gagtcagcaa actggttgaa
     2401 ataaaccagt aaggtttgtc gctggtcgat ggccgggtga tttttcagca tctcacgtac
     2461 ggcttcgaca ataacgccca cttttgccgc atcctcataa cgtaaaccaa tggtcgtggt
     2521 aatgcggcgg ttggtcattc gtcctgggtt ttctacgctg atcgacgaaa acagcgagtt
     2581 cggtacgtac aatggacgat tatcaaaggt cgtaattttg gtaattcgcc agccaatttc
     2641 cgctactgta ccttcgatat ttctgtccgg tgaacggatc cagtcgccaa tactgaaagg
     2701 acggtcgaaa tagagcatga tcccggaaaa gaaattactc agaatatctt taccggccat
     2761 accgacagcc agaccaccaa taccaccaaa ggtcagcaag ccagaaaggc tcatgccgaa
     2821 atgttcgcca taaagcagaa caagcactac aataatggtg attttgataa tgcgcgacat
     2881 aatccgcgca ctggtgatat cgcgaccttt tttaatctgc tgtttttcaa actgattaat
     2941 cagcagaaat agcttaatcg tcagaataac cgcaatcagg gacgtacaga taaaatcgat
     3001 aacgcctggg gtgataaat
//
LOCUS       DAJTRC010000216         2913 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.226, whole genome shotgun sequence.
ACCESSION   DAJTRC010000216 DAJTRC010000000
VERSION     DAJTRC010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.226"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..342
                     /locus_tag="OO357_004080"
     CDS             <1..342
                     /locus_tag="OO357_004080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5888808.1"
                     /translation="GWEHDNGNVEASFRLWLETRDNGTVPNFPNLAKVGSFAGTATTG
                     VGIRIDDAESGNLMPLNAMGNDNTIYQIPADSNGIVNVDLIAYYVSTVEASEITPGEA
                     DAVVNVTLDYR"
     gene            422..1117
                     /locus_tag="OO357_004081"
     CDS             422..1117
                     /locus_tag="OO357_004081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_008439285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone"
                     /protein_id="HCR5888809.1"
                     /translation="MSKRTFAVILTLLCSFCIGQALAGGIVLQRTRVIYDASRKEAAL
                     PVANKGAETPYLLQSWVDNIDGKSRAPFIITPPLFRLEASDDSSLRIIKTADNLPENK
                     ESLFYINVRAIPAKKKSDDVNANELTLVFKTRVKMFYRPAHLKGRVNDAWKSLEFKRS
                     DHSLNIYNPTEYYVVFAGLAVDKTDLTSKIEYIAPGEHKQLPLPASGGKNVKWAAIND
                     YGGSSGTETRPLQ"
     gene            1146..>2913
                     /locus_tag="OO357_004082"
     CDS             1146..>2913
                     /locus_tag="OO357_004082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5888810.1"
                     /translation="MPQRHHQGHKRTPKQLALIIKRCLPMVLTGSGMLCTTANAEEYY
                     FDPIMLETTKSGMQTTDLSRFSKKYAQLPGTYQVDIWLNKKKVSQKKITFTANAEQLL
                     QPQFTVEQLRELGIKVDEIPALAEKDDDSVINSLEQIIPGTAAEFDFNHQRLNLSIPQ
                     IALYRDARGYVSPSRWDDGIPTLFTNYSFTGSDNRYRQGNRSQRQYLNMQNGANFGPW
                     RLRNYSTWTRNDQASSWNTISSYLQRDIKALKSQLLLGESATSGSIFSSYAFTGVQLA
                     SDDNMLPNSQRGFAPTVRGIANSSAIVTIRQNGYVIYQSNVPAGAFEINDLYPSSNSG
                     DLEVTIEESDGTQRRFIQPYSSLPMMQRPGHLKYSATAGRYRADANSDSKEPEFAEAT
                     AIYGLNNTFTLYGGLLGSEDYYALGIGIGGTLGALGALSMDINRADTQFDNQHSFHGY
                     QWRTQYIKDIPETNTNIAVSYYRYTNDGYFSFDEANTRNWGYNSRQKSEIQFNISQTI
                     FDGVSLYASGSQQDYWGNNEKNSNISVGVSGQQWGIGYSLNYQYSRYTDENNDRALSL
                     NLSIPLERWWICPYISRHLLSLN"
BASE COUNT          844 a          686 c          654 g          729 t
ORIGIN      
        1 gggtgggagc acgataacgg caacgttgaa gcgtctttcc gtttatggct ggaaacgcgg
       61 gataatggca ccgtccctaa cttccctaat ctggcaaaag tgggctcttt tgctggcaca
      121 gcgacaacag gtgtgggtat ccgtattgac gatgcggaaa gcggaaacct tatgccactg
      181 aatgctatgg ggaatgataa cacgatttat cagatcccag cggactccaa tggtattgtc
      241 aatgttgacc tcatcgctta ctacgtatca accgttgaag catcagaaat caccccaggg
      301 gaagcagacg ctgtcgttaa cgtaacgctg gattaccgtt aatacgttac ggcgttatct
      361 gacctttcag ataacgccct tttccttcct ctttctcgtt gtatcaggtt gaaaaataac
      421 tatgtcaaaa cgaacattcg cggtgatatt aaccttgttg tgtagcttct gtatcggcca
      481 ggcgcttgca gggggtatcg ttttacagcg aacgcgagtg atctatgatg ccagccgcaa
      541 agaggctgcg ttacctgtcg caaacaaagg cgcagaaacg ccttatttac tgcaatcatg
      601 ggtagataat atagatggta aaagccgtgc cccatttatt ataaccccac cgctatttcg
      661 tcttgaggct agcgatgact catcactgcg aattattaaa acagctgata acctgcctga
      721 aaataaagag tcgctgttct acattaatgt tcgtgccatt ccagcaaaga aaaaatcaga
      781 tgatgttaat gctaacgagt tgacgctggt atttaaaacg cgggtcaaaa tgttttatcg
      841 ccccgcacac ctgaagggac gggtaaacga tgcgtggaaa tcactggaat ttaaacgtag
      901 tgaccattca ctcaatatat ataacccaac tgaatattac gtcgtatttg ccggactggc
      961 agtcgataaa accgatctca caagcaaaat tgaatatatc gcgcccggag aacataaaca
     1021 gttaccactt ccagcatctg gcggaaagaa cgtgaaatgg gctgcgatca acgattatgg
     1081 cggcagttcc gggacagaaa ctcgtccact gcaataaaaa atataaaaac acaggccatc
     1141 agggaatgcc acaacgacac caccagggac ataaacgcac accgaaacag ttggcgctca
     1201 ttatcaaacg ttgtttgccg atggtgctca ctggcagcgg catgctttgc actaccgcta
     1261 acgccgaaga gtattatttc gaccccatta tgctggaaac tacaaaaagt ggtatgcaaa
     1321 caaccgatct gtcacgtttt tcaaaaaaat acgcacaact accaggaact tatcaggttg
     1381 atatctggct gaataaaaag aaggtttcac agaaaaaaat tacatttacc gccaatgcag
     1441 agcaacttct gcagccacag tttacggtag aacaactacg tgagctgggt attaaggtgg
     1501 atgaaatccc ggcgctggct gaaaaagatg acgatagcgt gatcaactcg cttgaacaaa
     1561 tcattcccgg tacagctgct gaatttgatt tcaatcatca gcgacttaat ttgagcattc
     1621 cccaaattgc actgtaccgt gatgcaagag gttacgtctc cccttctcgt tgggacgatg
     1681 gtataccaac gctgtttacc aactactcgt ttacaggttc tgataaccgt taccgccagg
     1741 gcaatcgtag ccaacgacag tacctgaata tgcaaaatgg tgctaatttt ggcccctggc
     1801 gattacgtaa ctattctacg tggacacgca acgatcaggc gtcaagctgg aacactatca
     1861 gtagttattt acaacgtgat atcaaggcgt tgaagtctca gttgcttctg ggagaaagcg
     1921 ccaccagcgg cagtattttt tccagctacg cctttactgg cgtgcaactc gcttccgacg
     1981 ataatatgtt gccaaacagc cagcgcggat ttgccccaac ggtacgcggt atcgcaaaca
     2041 gtagtgcaat cgtgactatc aggcaaaatg gttatgtgat ctatcaaagc aacgtgccag
     2101 cgggtgcctt tgaaattaac gatctctacc cctcttccaa cagcggcgat ttagaagtca
     2161 cgattgaaga aagtgacggt acacaacgtc gctttatcca gccttattct tcattaccca
     2221 tgatgcagcg acctgggcat ctaaaatata gcgcgaccgc tggacgctat cgcgctgatg
     2281 caaacagtga tagcaaggaa cccgaatttg ctgaagccac ggcaatatat ggtttgaata
     2341 atacttttac gctgtatggc ggcctgctcg gttctgaaga ttattatgcg ctggggatcg
     2401 gtatcggcgg cacacttggc gcactgggcg cgttgtcgat ggatatcaac agagctgaca
     2461 cccaattcga taaccagcac tcttttcatg gctatcaatg gcgtactcag tacatcaaag
     2521 atatcccgga aaccaacacc aatatcgctg ttagctacta tcgctatacc aacgatggct
     2581 attttagttt tgatgaagcc aatacccgca attggggcta taacagtcgc caaaaaagtg
     2641 aaattcaatt caacatcagc cagacaatat ttgatggggt aagtctgtat gcctccggtt
     2701 cacagcaaga ctattggggc aataacgaga aaaacagtaa tatctctgtt ggggtttccg
     2761 gccagcaatg gggaattggt tacagcctga attatcaata cagccgctac actgatgaaa
     2821 ataatgaccg tgcactctct ttgaatctca gtattccgtt agaacgctgg tggatttgcc
     2881 cctatatttc cagacatctg ttatcactta acc
//
LOCUS       DAJTRC010000217         2810 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.227, whole genome shotgun sequence.
ACCESSION   DAJTRC010000217 DAJTRC010000000
VERSION     DAJTRC010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2810
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.227"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..681
                     /gene="ospC1"
                     /locus_tag="OO357_004083"
     CDS             <1..681
                     /gene="ospC1"
                     /locus_tag="OO357_004083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR5888811.1"
                     /translation="DKFSEVNKEVSRYVHGSKGIIDVPIFNTKDMKLGLGLYLIDFIR
                     KSEDQSFKEFCYGKNLAPVDLDRIINFVFQPEYHIPRMVSTENFKKVKIREISLEEAV
                     TASNYEEINKQVTNKKIALQALFLSITNQKEDVALYILSNFEITRQDVISIKHELYDI
                     EYLLSAHNSSCKVLEYFINKGLVDVNTKFKKTNSGDCMLDNAIKYENAEMIKLLLKYG
                     ATSDNKYI"
     gene            complement(785..934)
                     /locus_tag="OO357_004084"
                     /pseudo
     CDS             complement(785..934)
                     /locus_tag="OO357_004084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1010..2707
                     /gene="ospD3"
                     /locus_tag="OO357_004085"
     CDS             1010..2707
                     /gene="ospD3"
                     /locus_tag="OO357_004085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspD3"
                     /protein_id="HCR5888812.1"
                     /translation="MPSVNLIPSRKICLQNMINKDNVSVETIQSLLHSKQLPYFSDKR
                     SFLLNLNCQVTDHSGRLIVCRHLASYWIAQFNKSSGHVDYHHFAFPDEIKNYVSVSEE
                     EKAINVPAIIYFVENGSWGDIIFYIFNEMIFHSEKSRALEISTSNHNMALGLKIKETK
                     NGGDFVIQLYDPNHTATHLRAEFNKFNLAKIKKLTVDNFLDEKHQKCYGLISDGMSIF
                     VDRHTPTSMSSIIRWPDNLLHPKVIYHAMRMGLTELIQKVTRVVQLSDLSDNTLELLL
                     AAKNDDGLSGLLLALQNGHSDTILAYGELLETSGLNLDKTVELLTAEGMGGRISGLSQ
                     ALQNGHAETIKTYGRLLKKRAINIEYNKLKNLLTAYYYDEVHRQIPGLMFALQNGHAD
                     AIRAYGELILSPPLLNSEDIVNLLASRRYDNVPGLLLALNNGQADAILAYGDILNEAK
                     LNLDKKAELLEAKDSNGLSGLFVALHNGCVETIIAYGKILHTADLTPHQASKLLAAEG
                     PNGVSGLIIAFQNRNFEAIKTYMEIIKNENITPEEIAEHLDKKNGSDFLEIMKNIKS"
BASE COUNT          991 a          467 c          535 g          817 t
ORIGIN      
        1 gataaatttt cagaggttaa taaagaagtt agtcgatacg tacatggaag taaaggaatt
       61 atagatgtac caatattcaa tactaaagat atgaagttag ggctcggatt atacctgatt
      121 gactttatta gaaaaagtga agaccaaagc ttcaaggagt tttgctatgg aaaaaatctt
      181 gcccctgtgg atctggatag aatcataaac tttgtttttc agccagagta ccatatacct
      241 aggatggtaa gtacagaaaa cttcaaaaaa gttaagatta gagaaatatc cttagaggag
      301 gctgttacag catctaatta cgaagaaatt aacaagcagg tcactaacaa aaaaattgct
      361 ctccaggctc tttttctttc gattactaat caaaaagagg atgtcgcctt atatatatta
      421 tctaattttg agataactag acaagatgtt atttccataa agcatgagtt gtatgatatt
      481 gagtatctac ttagcgctca taattcaagc tgtaaagtac ttgagtattt tatcaataag
      541 ggattggttg atgtaaacac aaagttcaaa aaaactaata gtggggattg tatgttggat
      601 aacgcaataa aatatgagaa tgcagaaatg ataaaactat tattgaaata tggtgcaaca
      661 tctgacaata aatatattta atcaaaattg aatatcgttt agcaaaaggc agtatttgca
      721 caaaatatcg atatattgtt tattgtcagt atggctcaat gtgataatag ttggaaagtt
      781 tgatgggttt cgccccgttg tagcggtagt cgaactggaa ggtcttcagg cactgcttac
      841 agcgatagag cagcccccct gaactggaat ggccgttccg gatacccccc tgagtttcag
      901 agtaacggtg acaaaccaca tcaatctttg ccatcaatca tccaaagggc aaagagtaca
      961 acaacactaa gtctgcgtca caacccatca atgaaaggaa tatatacata tgccatcagt
     1021 aaatttaatc ccatcaagga aaatatgttt gcaaaatatg ataaataaag acaacgtctc
     1081 tgttgagaca atccagtctc tattgcactc aaaacaattg ccatattttt ctgacaagag
     1141 gagtttttta ttaaatctaa attgccaagt taccgatcac tctggaagac ttattgtctg
     1201 tcgacattta gcttcctact ggatagcaca gtttaacaaa agtagtggtc acgtggatta
     1261 tcatcacttt gcttttccgg atgaaattaa aaattatgtt tcagtgagtg aagaagaaaa
     1321 ggctattaat gtgcctgcta ttatttattt tgttgaaaac ggttcatggg gagatattat
     1381 tttttatatt ttcaatgaaa tgatttttca ttccgaaaaa agcagagcac tagaaataag
     1441 tacatcaaat cacaatatgg cattaggctt gaagattaaa gaaactaaaa atggggggga
     1501 ttttgtcatt cagctttatg atcccaacca tacagcaact catttacgag cagagtttaa
     1561 caaatttaac ttagctaaaa taaaaaaact gactgtagat aattttcttg atgaaaaaca
     1621 tcagaaatgt tatggtctta tatccgacgg tatgtctata tttgtggaca gacatactcc
     1681 aacaagcatg tcctccataa tcagatggcc tgataattta cttcacccca aagttattta
     1741 tcacgcgatg cgtatgggat tgactgagct aatccaaaaa gtaacaagag tcgtacaact
     1801 atctgacctt tcagacaata cgttagaatt acttttggca gccaaaaatg acgatggttt
     1861 gtcaggattg cttttagctt tacaaaatgg gcattcagat acaatcttag catacggaga
     1921 actcttggaa acttctggac ttaaccttga taaaacggta gaactactaa ctgcggaagg
     1981 aatgggagga cgaatatcgg gtttatccca agcacttcaa aatgggcatg cagaaactat
     2041 caaaacatac ggaaggcttc tcaagaagag agcaataaat atcgaataca ataagctgaa
     2101 aaatttgctg accgcttatt attatgatga agtacacaga cagatacccg gactaatgtt
     2161 tgctcttcaa aatggacatg cagatgctat acgcgcatac ggtgagctca ttcttagccc
     2221 ccctctcctc aactcagagg atattgtaaa tttgctggcc tcaaggagat atgacaatgt
     2281 tcccggactt ctgttagcat tgaataatgg acaggctgat gcaatcttag cttatggtga
     2341 tatcttgaat gaggcaaaac ttaacttgga taaaaaagca gagctgttag aagcgaaaga
     2401 ttctaatggt ttatctggat tgtttgtagc cttgcataat ggatgtgtag aaacaattat
     2461 tgcttatggg aaaatacttc acactgcaga ccttactcca catcaggcat caaaattact
     2521 ggcagcagaa ggcccaaatg gggtatctgg attaattata gcttttcaaa ataggaattt
     2581 tgaggcaata aaaacttata tggaaataat aaaaaatgaa aatattacac ctgaagaaat
     2641 agcagaacac ttggacaaaa aaaatggaag tgattttcta gaaattatga agaatataaa
     2701 aagctgaata ttatctcgtg gtgatacact gaatttgagg taaccggctc atttaaaccg
     2761 tctggtctgt tacctccggt tccacaaaaa taatgtccat cattttgaag
//
LOCUS       DAJTRC010000218         2790 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.228, whole genome shotgun sequence.
ACCESSION   DAJTRC010000218 DAJTRC010000000
VERSION     DAJTRC010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2790)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2790)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2790
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.228"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            437..2089
                     /locus_tag="OO357_004086"
     CDS             437..2089
                     /locus_tag="OO357_004086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-sugar
                     hydrolase/5'-nucleotidase"
                     /protein_id="HCR5888813.1"
                     /translation="MIPLKKNITLIMFTLSLLTGNPAIAYETDKVYKITVLHTNDHHG
                     HFWRNNHGEYGLSSQKTLVDNIRQKVINNGGSVLLLSGGDINTGVPESDLQKAEPDIR
                     GMNLIGYDAMAVGNHEFDNPLNILRQQEKWATFPFLSANIYQKSTGRRLFSPWKIFIR
                     QNLKIAVIGLTTDDTAKTGNSEYFTDIEFRQPAAEARSVIDELNQQEKPDIIIAATHM
                     GHYDNGESGSNAPGDVEMARSLPTGSLAMIVGGHSQAPVCMASDNKKQWNYIPGTTCV
                     PDKQNGIWIVQAHEWGKYVGQADFEFCNGTMKLVNYQLHPVNLKMRITREDGKTEFSF
                     YTPEITEDPQMLSLLTPFQNKGKAQLDVKVGVVNGRLEGDRSKVRFVQTSMGHLILSA
                     LTERIDADFAVVSGGEIRDSIESGNITYKDILKVQPFGNTVVSIDLTGKEVADYLATV
                     AQMKPDSGAYPQFLNTSFVVKKGKIEMLKIKGKSVDLNKKYRMTTFSFNATGGDGYPR
                     IDNRPGYINTGFIDAEVLIEYIRKHSPLDAASYEPKGEVSWQ"
     gene            complement(2100..2270)
                     /locus_tag="OO357_004087"
                     /pseudo
     CDS             complement(2100..2270)
                     /locus_tag="OO357_004087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2403..2522)
                     /locus_tag="OO357_004088"
                     /pseudo
     CDS             complement(2403..2522)
                     /locus_tag="OO357_004088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase domain-containing protein"
BASE COUNT          833 a          570 c          644 g          743 t
ORIGIN      
        1 aatcgacgat tgctggaaag gctgggccac atccctccgg cagaagcaga aaaagcttat
       61 tatgcttcta tcggaaacga tgatctggca gcctgagttc acagataaaa cattctccag
      121 gaaacttggg gcggttcacg tcagtatcag accatattaa ttgctgaaac tgataaacat
      181 caaaccttat agaagggtga atgctgaaag ggggctatgg tgatcgacgt gatgacaacg
      241 acaggtaaga ccgtgacctg ataaacctga attgtgtcct agacataaag tccgtgaagg
      301 gaataaggct caggtcagcg aatcttatag tggcccgcag ctctgactgc aataaggccg
      361 gatacagagc agcaactact cgtcgatgtc agaacaacgg aagccttgca aacggggatg
      421 cgttcataca ggaacaatga taccgttaaa aaaaaacatt acgctaataa tgttcaccct
      481 gtcattactg acaggaaatc ctgctatagc gtatgaaaca gataaagtat ataaaataac
      541 cgttctgcat acaaatgacc accatggaca cttttggcgt aacaatcatg gtgagtacgg
      601 gttatcctcc caaaaaactc tggtggacaa tatccggcag aaagtaataa ataatggcgg
      661 aagtgttcta ttattatctg gtggtgatat taacaccggt gtacctgaat cagaccttca
      721 gaaggctgaa cccgatattc gtggaatgaa tctaattggt tatgatgcaa tggccgtcgg
      781 taatcatgaa tttgataatc ctctaaatat attacgtcag caggaaaaat gggccacatt
      841 tcctttcctt tctgctaaca tatatcaaaa aagtacaggg aggcgtttat tcagtccatg
      901 gaaaattttc atccgtcaga atttgaaaat tgcagtcatt ggcttgacga cagatgatac
      961 tgcgaaaaca ggaaattctg aatattttac tgatattgag ttccgtcaac ctgctgctga
     1021 ggcgaggagt gtcattgatg aacttaatca gcaggaaaag ccagatatta ttattgcggc
     1081 aacacatatg ggtcactatg acaatggcga gtcaggttcg aatgctcctg gtgatgttga
     1141 aatggctcgc agtttgccga ctggctcatt agcaatgatt gtgggaggac attcacaagc
     1201 tcctgtctgc atggccagcg ataataaaaa acagtggaat tatattccgg ggacaacctg
     1261 tgtaccagat aaacaaaatg gcatctggat tgtacaagcg catgagtggg gaaaatatgt
     1321 cggtcaggct gattttgaat tctgtaacgg tacaatgaaa ctggttaatt atcagttaca
     1381 cccagttaat ctcaaaatga gaattaccag agaagatgga aaaacggaat tttcttttta
     1441 cacccccgaa attactgaag atccacagat gttatcctta ctgacaccat tccagaataa
     1501 aggcaaagca caactggatg tgaaggttgg ggttgtgaac ggacgtctgg aaggggacag
     1561 aagtaaagtt cgttttgttc agaccagtat gggccatctg attctgagcg ctctgacaga
     1621 acgtattgac gcagattttg ctgttgtcag tggtggggaa attcgagatt ccatagagag
     1681 tggtaacatc acatacaaag atatactgaa agttcagcca tttggtaata cggtagtaag
     1741 tattgatttg acggggaaag aagtagctga ctacctagct acagtggcac agatgaaacc
     1801 ggattccgga gcctacccac aatttttgaa taccagcttt gtagtcaaaa aaggaaaaat
     1861 cgaaatgcta aaaataaaag gtaaatcggt tgaccttaac aaaaaatatc gtatgacaac
     1921 atttagcttt aatgctactg gtggggatgg ctatccacgt attgataata gacccggata
     1981 cataaatacc ggatttattg atgctgaagt acttatcgaa tatatccgga aacattcacc
     2041 gctggatgct gcttcatatg aaccaaaagg tgaagtcagt tggcagtgat aacgtaattg
     2101 acgatcatca cgtgtttttt gctgacagtc gctgtgactt tgtgaaagta gtctctgcgt
     2161 atgtttgcga tatgggaatg cagttgctgt attttgtgtt tctgcttctg ccagttgttg
     2221 ctgaatttaa ccttgcggct taactgatgt tggagcgctg ccagcttctt cgtcaatttc
     2281 gctgattaaa gtttattcat tatggcatga tctgcgtaag cggctcgtca gaaccatatt
     2341 gatatttact aagagctcag atcaactttc cagggcaaca gatcgcgtac ccggtttgcc
     2401 ggtcagtcct ggatatgctc aatgacgtaa cgcagccatt tttctggctc cacattgttc
     2461 agacggcatg tgccgatcag cgagtacaac accgccgcat gttcgccacc gctatcggaa
     2521 ccccgccgga gagagtttca ctctccggct gctcacggaa ccgtacgtga cactctcgcg
     2581 tcatacggct cttgatcact cattcgatgc acgtagtaaa taccagtggg caaatagctt
     2641 cggatactgt ttccttacca gcgccagctt tcgctgagcc ttccggttcg aaagtctgta
     2701 tttagactgc atccacttaa gcaaacgtga ctgcattgca ctccacagtc gatagctgaa
     2761 gtttctggac cagaactttc catagtactc
//
LOCUS       DAJTRC010000219         2628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.229, whole genome shotgun sequence.
ACCESSION   DAJTRC010000219 DAJTRC010000000
VERSION     DAJTRC010000219.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.229"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..545)
                     /locus_tag="OO357_004089"
     CDS             complement(<1..545)
                     /locus_tag="OO357_004089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001338841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888814.1"
                     /translation="MDYFMKGKSALTLLLAGIFSCGTCQATEAEVTSESVFNILNSRG
                     AATDKSYLSLNPDKYPNYRLLIHSAKLKNEIKSHYTKDETQGLLTLTENTRKLTLTEK
                     PWGTFILASTFEDDKTAAETHYDAVWLRDSLWGYMALVSDQGNSVAAKKVLLTLWGYM
                     STPDQIKRMQDIISNPKRLDG"
     gene            complement(1207..2196)
                     /locus_tag="OO357_004090"
     CDS             complement(1207..2196)
                     /locus_tag="OO357_004090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888815.1"
                     /translation="MNMTKNAFQFGIEPVRITDTDNIQVNEGLPTNADPQVYALQLAK
                     TVKAMLNGVLKDAQDNIPFPVEVLPTRNSLPTPIIAHTLADRSVLVPVRGGKRPEVVT
                     APSGTEITVEPIEQAILVSHQTKLWDQKSTTGFTQGTLQQDALNICDNVIRTINSKMV
                     DVLESSKLLKTVELPALTGSLTAKADAIMDALYENTESSFGSEVSDYGIIAHESHLKA
                     LSRLAAKQGFGGEDAIVDMLGTDVAYYNGEDRGVFMMAKRFTALSFGCFRHDGESITV
                     VLSRDGDSQSHDLEILGKVFVVAEAATTIKMGTGSATAVLPVVKRLSFTKEAN"
     gene            complement(2280..>2628)
                     /locus_tag="OO357_004091"
     CDS             complement(2280..>2628)
                     /locus_tag="OO357_004091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888816.1"
                     /translation="TSREAMELLEALAEAGVTIGNFFSNDLMTAYQRRQMNKRAEAER
                     IAKELASQKERTREMFMTEDEWQKELQRREQVKAERRTYGENLRSATHSAGRSRAAIV
                     ADLESGGNWMDSL"
BASE COUNT          682 a          613 c          632 g          701 t
ORIGIN      
        1 attccatcca gtcgttttgg gttgctaatg atgtcctgca tccgcttaat ctggtcgggc
       61 gtagacatat aaccccacag ggtgagcaga accttttttg cggctacact atttccttgg
      121 tctgatacta atgccatata gccccataaa ctatccctca gccagacagc atcatagtgg
      181 gtttctgccg ctgttttgtc atcttcgaaa gtagaagcaa gaataaacgt tccccagggt
      241 ttctccgtca acgttaactt tcgtgtgttt tctgttaatg ttagcaatcc ttgagtttcg
      301 tctttggtat agtgactctt tatttcattt tttaatttgg ctgagtgaat cagaagacga
      361 taattaggat acttatctgg atttaacgat aaataacttt tatctgttgc tgcccccctg
      421 gaattaagaa tgttaaatac agattcgctt gtcacttctg cctctgtcgc ctggcatgtg
      481 ccgcacgaaa atataccagc gagcagaaga gttaatgcag atttcccttt cataaaataa
      541 tccatcaatc gtttgttaaa agcgtagata accacttatt caacaacttg aaaataaggt
      601 attttacctg agttgttaca aaaggattgc attgcgtaaa cgctttttat ttacaaaaaa
      661 atggggaagt gttacagcga ttgcgaaaag cctgaagtga ttgaattaag ataatataga
      721 aaatcagata cagcaaaaat ggcagattga tcaggtgaag ataaattgga gcgggcgaag
      781 ggagcctacg acaaatcgaa gacaaccagc taacctattg tttcttaaag acaggtgaat
      841 ccagttggtt gtagtgatag tcacacattt agagatagaa ggtcaagact tgaagcacgt
      901 aatattccct ctaaaggaat agaactcacg gtctgactac ccggcccttt tggtctggct
      961 caggtcaggc tatgggggct tttggctcgc tctttatatt agatgaggtc tcacattttt
     1021 cagtcaaaac tcatcgtcta atagcccata gccagaccac cagtcccagc tttagcagga
     1081 agaccacgag atagactttg tgtaggtcca tttggccctc ctactttctg tattcacaaa
     1141 ctagattact agtacaaaat aaaggccaga catccagttc ggaagtctga ccaatagtcg
     1201 ttattgttag ttagcttctt tagtgaagct caggcgtttg actacaggca ggacagcagt
     1261 agccgagcca gtacccatct tgatggtcgt agcagcttca gctacaacaa acaccttgcc
     1321 gagaatctca aggtcgtgag actgggagtc tccatcacgt gaaaggacca cagtaatgct
     1381 ctcgccatcg tgacggaagc aaccgaagct cagagccgta aaacgcttag ccatcatgaa
     1441 cacgccacgg tcttcaccgt tgtaataggc gacgtcagtc cccagcatat cgacgatagc
     1501 gtcttcacca ccgaagccct gcttagcagc cagacgggag agagccttga ggtgtgactc
     1561 atgggcgata atgccgtagt cagaaacctc agaaccgaaa gaagactccg tgttctcgta
     1621 cagggcatcc atgatagcgt cagccttagc ggtcaggcta ccagtcaggg ccggaagttc
     1681 tacggtcttc agcagctttg aggactccag cacgtcaacc atcttagagt taatggtacg
     1741 aatcacgtta tcgcagatgt tcagtgcgtc ctgctggagc gtcccctgag tgaaaccagt
     1801 ggtcgacttc tggtcccaaa gctttgtttg gtgagacacg aggatcgcct gttcaatcgg
     1861 ctcaacggtg atttcagtgc cagacggagc ggtcacaact tcaggtcgct taccaccacg
     1921 gacagggacc agcacagagc ggtcagccag agtgtgagca atgattggag tcggtaagct
     1981 gttacgggtc ggcaggactt ctacagggaa cgggatgtta tcttgagcgt ctttaagtac
     2041 accgttcagc atagccttaa cagtcttagc taactggagg gcatagacct gagggtcggc
     2101 gttagtcggc agaccttcat tgacctgaat gttgtcggta tcggtgatgc gtactggctc
     2161 aatgccaaac tggaaagcgt ttttagtcat attcattata attaatctcc ttgagtcaaa
     2221 tacttgaagt ccctaacagg cagtcaggga ccgtgtgttc tcagatgagg gacgcttgct
     2281 tacaagctgt ccatccagtt accgcctgac tcaaggtcag cgacgatagc ggcacgtgag
     2341 cgaccagcgg aatgagtagc gctgcggagg ttttcaccat aggtccggcg ttcagctttg
     2401 acctgttcgc ggcgctgtag ctctttttgc cattcgtctt cggtcatgaa catttcgcgg
     2461 gtacgctctt tctgagacgc tagctcttta gcgatacgct cagcttcagc tcgtttgttc
     2521 atctggcggc gttggtaggc ggtcatcagg tcattactga agaagttacc aatggtcacg
     2581 ccagcttcag ccagagcttc cagtaactcc atcgcctcgc ggctggtg
//
LOCUS       DAJTRC010000220         2576 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.230, whole genome shotgun sequence.
ACCESSION   DAJTRC010000220 DAJTRC010000000
VERSION     DAJTRC010000220.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2576
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.230"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            53..2575
                     /locus_tag="OO357_004092"
     CDS             53..2575
                     /locus_tag="OO357_004092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial biogenesis outer membrane usher
                     protein"
                     /protein_id="HCR5888817.1"
                     /translation="MMTTRIVVGLTAGTCLIFSQNLMAEVSVFNPALLEINHQSGVDI
                     RQFNRANLMPPGVYSVDIFINGKMFERQDVTFVQDNPDADLHACFIAIKKTLSSFGIK
                     VDALKSFNDVDETVCLDPAPRIEGSSWQFDSDKLQLNISIHQIYMDAMAYDYISPTRW
                     DEGINALTINYDFSGSHTLRSDYGSQETDTSYLNLRNGLNIGPWRLRNYSTLNTSDGR
                     AEYNSISTWIQRDIAALRSQIMIGDTWTASDIFDSTQIRGARLYTDNDMLPASQNGFA
                     PVVRGIAKSNATVIIRQNGYVIYQSAVPQGAFEITDLNTASTGGDLDVTIKEEDGSEQ
                     RFTQPYASLAILKREGLTDVDVSVGELRDEDGFTPDVLQAQILHGFSHGITLYGGMQA
                     AENYGSAALGVGKDLGALGAISFDVTHARANFSHDDTETGQSYRFLYSKLFDDTDTSL
                     RLVGYRYSTEGYYTLNEWASRRNSPEDFWETGNRRSRVEGTLTQSLGRDYGNLYLTLS
                     RQQYWHTDDVERLMQFGYSSSWKRLSWNVSWSYSNTARQGTGNNHASDNTSEQIYMLS
                     LSVPLSGWWGNSYATYSVSQNDNSGSSHQLGLSGTALERNNLSWNLMQSYNSHDDEVG
                     GNMSLTYDGSYGTVNGSYNYSQNSQRLNYGIRGGILAHSEGVTLSQELGETIALVKAP
                     GAARLEIDNMRGAATDWRGYTVKTQLNPYDENRVAISDNYFSKSNIELDNTVVTMVPT
                     RGAVVKAEFVTHVGYRVLFRVLNANGKPVPFGAIAAIQDASLADSGIVGDRGELYLSG
                     LPEKGQVTLSWGENASTKCIFNYSFSTPESESGLIEQGVTCH"
BASE COUNT          694 a          560 c          648 g          674 t
ORIGIN      
        1 cagtgacccg ataaaaatga cgctgtaagc aagggataag gataggcgta ttatgatgac
       61 gaccagaata gtggttggcc tcacggcagg gacgtgtctg attttctcgc aaaacctgat
      121 ggccgaggtc agtgtattca atccggcgct tctggaaatc aaccatcaat ccggagtcga
      181 tattcgccag tttaatcggg caaacctgat gcccccaggt gtttatagcg ttgatatttt
      241 tatcaacggt aaaatgtttg aacgtcagga tgtgacattt gttcaggata atccagatgc
      301 tgatctgcac gcttgcttta ttgccattaa aaaaacactg tcctcctttg gcataaaagt
      361 tgatgcgctc aaatcgttca atgatgtgga tgagacggtt tgcctcgatc ctgctccacg
      421 tattgaaggc tcatcctggc agtttgacag tgataaattg cagctgaata tatccattca
      481 ccaaatctac atggacgcga tggcttatga ttacatcagc cccacgcgtt gggatgaggg
      541 gattaatgcg ctcaccatca actacgattt ttctggttca catacactac gttcagatta
      601 tggttcacaa gagacagata ccagttatct caatctgcgc aatggactga atattggacc
      661 gtggcggcta cgtaattaca gtactttaaa caccagcgat ggccgtgcgg aatacaactc
      721 cattagtacc tggatacagc gcgatattgc cgcgttaaga agccagatta tgattggtga
      781 tacgtggacg gcgagcgata ttttcgacag tacgcaaatt cgcggcgcgc gtttgtatac
      841 tgataacgat atgctacccg ccagccagaa tggctttgct cctgtggttc gtgggattgc
      901 aaagtccaac gccaccgtca tcattcggca gaatggctac gtgatttatc agtcagccgt
      961 tccacaaggt gcttttgaga tcaccgatct caacaccgca agtacaggtg gcgatttgga
     1021 cgtaaccatc aaagaagaag acggtagcga acaacgattc acccaacctt atgcttcatt
     1081 ggcgattctt aaacgtgaag gtctgacaga tgttgatgtc agcgtgggtg aattgcgcga
     1141 tgaagacgga tttacaccgg acgtccttca ggcgcaaata cttcatggtt tttcccacgg
     1201 gatcacttta tatggaggta tgcaggctgc tgaaaattat ggttctgcag ctctgggtgt
     1261 cggtaaagat cttggcgctt tgggcgcaat ttctttcgat gtgacacatg ctcgtgcgaa
     1321 ttttagccat gatgatacag aaacgggtca gtcatatcgc tttctctatt caaaactatt
     1381 tgacgacaca gacactagct tgcgcctggt tggctatcgt tactccaccg agggctacta
     1441 taccctcaat gagtgggcat cgcggcgcaa cagccctgaa gacttttggg aaacaggtaa
     1501 ccgacgtagt cgcgtggagg gaacgctaac gcagtcgttg gggagagatt atggcaattt
     1561 atacctgaca ttaagccggc aacaatactg gcataccgat gatgtcgaac gattaatgca
     1621 atttggctac agcagtagct ggaagcgtct ctcgtggaac gtctcctgga gttattccaa
     1681 tactgccaga caggggacgg ggaacaacca tgccagtgat aacaccagtg agcagatcta
     1741 catgctctct ttatctgttc ctttatcggg ctggtggggt aatagttacg ccacctattc
     1801 tgtttcgcaa aacgataatt ccggtagctc acatcaactc ggactcagcg gtacggcgct
     1861 ggaaagaaat aacctttcat ggaatttaat gcagtcctat aacagtcatg atgatgaggt
     1921 tggcggtaat atgtccctga cctatgatgg ctcttatggc acggtgaacg gcagctataa
     1981 ctacagccaa aattcccaga ggctgaatta tggtatcaga gggggaattc tggcacacag
     2041 cgaaggggta acgttaagtc aggagttagg tgaaactatt gctcttgtta aagcacctgg
     2101 ggccgccagg ttagaaatag ataatatgcg cggtgctgcg acggactggc gcggctatac
     2161 ggtcaagaca cagctaaacc cttatgatga aaatcgggta gcaatcagcg ataactattt
     2221 ctcgaagtcg aatatagaac ttgataatac cgtcgttacg atggttccca cgcgtggtgc
     2281 agtggttaaa gcggagtttg tgactcatgt gggttatcgc gttctcttca gagtgttaaa
     2341 tgcaaatggt aaaccggtac cttttggagc cattgctgcg atacaagatg caagtttggc
     2401 agattcagga attgtcggtg accgtggcga actttatctt tctggtctac cagaaaaagg
     2461 acaggttacg ttatcctggg gagaaaacgc ctcaacaaaa tgcatcttca attattcatt
     2521 ttcgacacca gaaagtgaga gcggattaat tgaacagggt gtgacatgtc attaaa
//
LOCUS       DAJTRC010000221         2548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.231, whole genome shotgun sequence.
ACCESSION   DAJTRC010000221 DAJTRC010000000
VERSION     DAJTRC010000221.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.231"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..565
                     /locus_tag="OO357_004093"
     CDS             <1..565
                     /locus_tag="OO357_004093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HCR5888818.1"
                     /translation="VIADLAGESEDPRSVCEHYYSLISQYREIHWVFMVSRSWYSQAV
                     ELLMCPTATLLSDVEPIENLVKTVRSGNTHAERISAMLTSPAMTETHDFSYRSVILTL
                     SERKVLRLLGKGWGINQIASLLKKSNKTISAQKNSAMRRLAIHSNAEMYAWINSAQGA
                     RELNLPSVYGDAAEWNTAELRREMSHS"
     gene            523..1200
                     /gene="bglJ"
                     /locus_tag="OO357_004094"
     CDS             523..1200
                     /gene="bglJ"
                     /locus_tag="OO357_004094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710107.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding transcriptional activator BglJ"
                     /protein_id="HCR5888819.1"
                     /translation="MEHSRIKKRNVALIEKCVMSSIGIESLFRKFAGNPYKLHTYTSQ
                     ESFQDAMSRISFAAVIFSFSAMRSERREGLSCLTELAIKFPRTRRLVIVDDDIEARLI
                     GSLSPSPLDGVLSKASTLEIFHQELFLSLNGVRQATDRLNNQWYINQSRTLSPTEREI
                     LRFMSRGYSMTQIAEQLKRNIKTIRAHKFNVMSKLGVSSDAGLLEAEDILLCMRHCEA
                     SNVLHPY"
     gene            complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="OO357_004095"
     CDS             complement(1238..2026)
                     /gene="fhuF"
                     /locus_tag="OO357_004095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore-iron reductase FhuF"
                     /protein_id="HCR5888820.1"
                     /translation="MAYRSAPLYEDVIWRTHLQPQDAGLAQAVRAMIAKHREHLLEFI
                     RLDEPAPLNAMTLAQWSSPNALSSLLAVYSDHIYRNQPTMIRENKPLISLWAQWYIGL
                     MVPPLMLALLTQEKALDVSPEHFHAEFHETGRVACFWVDVCEDKNATPHSPQQRMETL
                     ISQALVPVVQALEATGEINGKLIWSNTGYLINWYLTEMKQLLGEATVESLRHALFFEK
                     TLTNGEDNPLWRTVVLRDGLLVRRTCCQRYRLPDVQQCGYCTLK"
     gene            2167..2403
                     /locus_tag="OO357_004096"
     CDS             2167..2403
                     /locus_tag="OO357_004096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313355.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1435 domain-containing protein"
                     /protein_id="HCR5888821.1"
                     /translation="MLQRTLGSGWGVLLPGLLIAGLMYADLSPDQWRIVILMGLVLTP
                     VMLYHKQLRHYILLPSCLALIAGIMLMIMNLNQG"
     gene            complement(2442..2528)
                     /locus_tag="OO357_004097"
     tRNA            complement(2442..2528)
                     /locus_tag="OO357_004097"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2492..2494),aa:Leu,seq:cag)
BASE COUNT          652 a          567 c          652 g          677 t
ORIGIN      
        1 agtcattgct gatttagccg gtgaaagtga agatccacgt tctgtttgtg aacattatta
       61 ttctttaatc tcacaatatc gggaaattca ctgggttttc atggtgtcac gctcctggta
      121 ttcccaggct gtagaactgc tcatgtgccc tacggcgacg ttattgtctg atgttgaacc
      181 cattgagaat ctggtcaaga ccgtacgttc cggcaatacg cacgcagagc gtatcagcgc
      241 catgctgacc tctccggcaa tgactgaaac tcatgatttt agctatcgct ccgtcattct
      301 cactctttca gagcgcaagg tactgcggct attaggtaaa ggatggggca tcaaccagat
      361 agcttcattg cttaagaaaa gtaataaaac tatcagcgcc caaaaaaaca gtgcgatgcg
      421 tcgactggca attcacagca acgctgaaat gtatgcatgg ataaatagcg cgcagggtgc
      481 aagagaactt aacttgcctt ctgtttatgg agatgccgca gaatggaaca cagccgaatt
      541 aagaagagaa atgtcgcact catagaaaaa tgcgtcatga gtagtatcgg tattgagagt
      601 ttattcagaa agtttgcggg caacccttat aagctccata cctataccag tcaggagtca
      661 tttcaggatg ccatgtcgcg gatctcgttt gcggcggtca ttttttcttt ttctgccatg
      721 agaagtgagc gcagagaggg attatcttgc ctgactgagc tggcgattaa gtttccgcgt
      781 acccggcgtt tagttattgt ggatgatgat attgaagctc ggctgattgg ttcattgtcg
      841 ccatcaccgc tggacggtgt attaagtaaa gcgtcaacgc tggagatttt tcatcaggaa
      901 ctctttttgt cattaaatgg tgtacgtcag gcgaccgacc gactgaacaa tcagtggtac
      961 attaaccaaa gccggacgtt aagcccgacg gagagagaaa tattgcgctt tatgtcgcgt
     1021 ggctactcaa tgacacaaat tgccgagcag cttaaacgta atatcaaaac gatccgagcg
     1081 cataaattta atgtgatgtc gaaactgggc gtcagttctg atgcagggtt gttggaggcc
     1141 gaagatattc tgttatgtat gcggcattgc gaagcaagta atgtgttgca tccctattaa
     1201 tccgcatgat gccgggttta cttccccggc agtgctttca tttcagcgta caatagccac
     1261 attgctgcac atccggtaag cgataacgct ggcagcaagt gcggcgcacc agcaggccgt
     1321 cgcgcagtac cacggtacgc cagagtggat tatcttcacc gttcgtgagc gttttctcaa
     1381 aaaagagggc atggcgcagc gattcaacag tagcctcgcc gagcagttgc ttcatctcag
     1441 tgagatacca gttgatcaaa taaccggtat tactccagat aagtttgccg ttgatctctc
     1501 cggtcgcttc tagtgcttgc acaaccggaa ccagcgcctg gctgattaac gtttccattc
     1561 gctgctgcgg cgaatgtggt gttgcgtttt tatcttcaca cacatcgacc cagaaacagg
     1621 cgacgcgtcc ggtttcgtga aactcagcat ggaaatgttc cggcgacaca tctaatgctt
     1681 tttcctgcgt cagtagcgcc agcattaatg gtggcaccat caggccgata taccattgtg
     1741 cccatagtga gatcagcggt ttgttctcgc ggatcatcgt tggttggttg cgatagatat
     1801 gatcggaata gaccgccagt agagaactta gcgcattcgg tgatgaccat tgcgccagcg
     1861 tcatggcgtt aagcggagcg ggttcatcca ggcggataaa ctccagcaaa tgttcacgat
     1921 gtttggcgat catcgcccgt acggcttgtg caagcccagc atcctgcggc tggagatgcg
     1981 ttcgccagat gacatcttca tagagcggtg cggaacgata ggccataatc gtgatagtaa
     2041 tctaaatgat aatgattgct aatcctagcg ataggtttac tcgatagcaa gggatttctc
     2101 tggcttgcaa atgataaaaa ttatcatatg atattggttt tcattatcaa tgaaagagat
     2161 gaaaccatgt tgcaacgtac gctgggcagt ggctggggag tgttgctgcc gggattgctg
     2221 attgcagggc tgatgtatgc ggatttatcg ccggatcagt ggcggattgt cattctgatg
     2281 ggattagtat tgacgccggt gatgctgtat cacaaacagt tgcggcatta cattttgcta
     2341 ccatcgtgcc tggcgcttat tgctggcatc atgctgatga taatgaattt gaatcaggga
     2401 tgacaaaaca gggaagaaac aagaaaggaa gtaaagataa ttggtgcgag gggggggact
     2461 tgaaccccca cgtccgtaag gacactaaca cctgaagcta gcgcgtctac caattccgcc
     2521 accttcgcac agtcatctta cttttttt
//
LOCUS       DAJTRC010000222         2491 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.232, whole genome shotgun sequence.
ACCESSION   DAJTRC010000222 DAJTRC010000000
VERSION     DAJTRC010000222.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.232"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..1549
                     /gene="kup"
                     /locus_tag="OO357_004098"
     CDS             <1..1549
                     /gene="kup"
                     /locus_tag="OO357_004098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="low affinity potassium transporter Kup"
                     /protein_id="HCR5888822.1"
                     /translation="IGGSFFYGEVVITPAISVMSAIEGLEIVAPQLDTWIVPLSIIVL
                     TLLFMIQKHGTAMVGKLFAPIMLTWFLILAGLGLRSIIANPEVLHALNPMWAVHFFLE
                     YKTVSFIALGAVVLSITGGEVLYADMGHFGKFSIRLAWFTVVLPSLTLNYFGQGALLL
                     KNPEAIKNPFFLLAPDWALIPLLIIAALATVIASQAVISGVFSLTRQAVRLGYLSPMR
                     IIHTSEMESGQIYIPFVNWMLYVAVVIVIVSFEHSSNLAAAYGIAVTGTMVLTSILST
                     TVARQNWHWNKYFVALILIAFLCVDIPLFTANLDKLLSGGWLPLSLGTVMFIVMTTWK
                     SERFRLLRRMHEHGNSLEAMIASLEKSPPVRVPGTAVYMSRAINVIPFALMHNLKHNK
                     VLHERVILLTLRTEDAPYVHNVRRVQIEQLSPTFWRVVASYGWRETPNVEEVFHRCGL
                     EGLSCRMMETSFFMSHESLILGKRPWYLRLRGKLYLLLQRNALRAPDQFEIPPNRVIE
                     LGTQVEI"
     gene            1716..2135
                     /gene="rbsD"
                     /locus_tag="OO357_004099"
     CDS             1716..2135
                     /gene="rbsD"
                     /locus_tag="OO357_004099"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709562.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HCR5888823.1"
                     /translation="MKKGTVLNSDISSVISRLGHTDTLVVCDAGLPIPKSTTRIDMAL
                     TQGVPSFMQVLGVVTNEMQVEAAIIAEEIKQHNPQLHETLLTHLEQLQKHQGNTIEIR
                     YTTHEQFKQQTAESQAVIRSGECSPYANIILCAGVTF"
     gene            2143..>2491
                     /locus_tag="OO357_004100"
     CDS             2143..>2491
                     /locus_tag="OO357_004100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HCR5888824.1"
                     /translation="MEALLQLKGIDKAFPGVKALSGAALNVYPGRVMALVGENGAGKS
                     TMMKVLTGIYTRDAGTLLWLGKETTFTGPKSSQEAGIGIIHQELNLIPQLTIAENIFL
                     GREFVNRFGKIDWK"
BASE COUNT          566 a          603 c          651 g          671 t
ORIGIN      
        1 aatcggcggc agctttttct atggtgaagt cgtcataaca cccgctattt cggtgatgtc
       61 agccattgaa ggtctggaaa tcgtcgcccc gcagctggat acctggatag ttcccctctc
      121 aattatcgtt ctcactttat tatttatgat tcaaaagcat ggcacggcta tggtcggtaa
      181 gctgtttgcg ccgatcatgc tgacctggtt tttgattctg gcaggactgg ggttacgtag
      241 cattattgct aacccggaag tgctgcatgc gctgaatccg atgtgggcgg tgcatttctt
      301 ccttgaatac aaaacggttt cttttattgc attaggggca gtggtgctgt cgattacggg
      361 gggcgaggtg ctgtatgctg atatggggca ctttggtaag ttctctattc gcctggcgtg
      421 gttcaccgtc gtattacctt ccttgaccct taattacttc ggtcagggag cgttgttgtt
      481 aaagaacccg gaagcgatta agaacccgtt cttcctgttg gcaccggact gggcgctgat
      541 cccactgctg atcatcgccg cactggcgac ggtaattgcc tcgcaggcgg ttatctctgg
      601 cgtcttctca ttgacgcgtc aggcggtacg tctgggatat ttgtcgccga tgcgcattat
      661 tcacacctcc gaaatggagt cagggcaaat ctatattccg tttgtgaact ggatgctcta
      721 tgtcgcggtc gtgattgtga ttgtcagctt tgagcactcc agcaacctgg cggcggcgta
      781 cgggattgcg gtgaccggaa ccatggtact gacgtctatt ctctcgacta ccgtggcacg
      841 tcagaactgg cactggaata agtattttgt tgcgctgatc ctgattgctt tcctttgtgt
      901 cgatattcca ttgttcaccg ctaacctcga taaactgctc tccggcggct ggttgccatt
      961 gagcctcggt actgtgatgt ttatcgtgat gaccacctgg aagagcgagc gtttccgctt
     1021 gctgcggcgg atgcatgaac atggtaactc tctggaagcg atgattgctt cgctggagaa
     1081 atcaccgcct gttcgcgtgc ccgggaccgc ggtgtatatg tcgcgtgcaa tcaacgtcat
     1141 tccctttgcg ctgatgcata accttaaaca taacaaggta ttgcatgagc gggtgattct
     1201 gttaactctg cgcaccgaag acgcgccata tgtccataac gtccgtcggg tacagattga
     1261 acaactgtcg cccactttct ggcgcgtggt ggcaagttat ggttggcgag aaacgccaaa
     1321 cgtggaagaa gttttccacc gctgcggtct ggaaggatta agttgccgga tgatggaaac
     1381 ctccttcttt atgtcgcatg agtcgttgat cctcggcaaa cgcccgtggt atttgcgtct
     1441 gcgcggcaag ctgtacttgc tgctgcaacg taatgcgctg cgtgcaccag atcaatttga
     1501 aatcccgcca aacagggtta tcgaactggg tactcaggtc gaaatctaac gccagacgcc
     1561 tcctttcttc ataagggggc gtttttgttt tcatggttaa tcaccatgta aaacgtttcg
     1621 aggttgatca catttccgta acgtcacgat ggttttccca actcagtcag gattaagctg
     1681 cggatcagcg aaacgtttcg ctgatggaga aaaaaatgaa aaaaggcacc gttcttaatt
     1741 ctgatatttc atcggtgatc tcccgtctgg gacataccga tacgctggtg gtgtgtgatg
     1801 ctggtttacc catccccaaa agtacaacgc gtatcgatat ggcattaacc caaggtgtac
     1861 cttcttttat gcaggtgctg ggcgtcgtca caaatgaaat gcaggtcgag gctgccatta
     1921 tcgcggaaga gatcaaacaa cataatccgc aactccacga aacgttgctc actcaccttg
     1981 agcagctgca aaaacaccag ggaaatacca ttgaaattcg ttacaccacg catgaacaat
     2041 tcaaacaaca aaccgcagaa agtcaggcgg taattcgcag cggagaatgt tctccgtatg
     2101 cgaatatcat tctctgtgct ggcgtgacgt tctgaggccg tcatggaagc attacttcag
     2161 cttaaaggca tcgataaagc cttcccgggc gtaaaagccc tctcgggcgc agcgttaaat
     2221 gtctatccgg gccgtgtgat ggcgctggtg ggcgaaaacg gcgcgggtaa atccaccatg
     2281 atgaaagtgc ttactggtat ctatactcgc gatgccggca cgcttttatg gctggggaaa
     2341 gaaacgacat ttaccgggcc gaaatcttcc caggaagccg ggattgggat tatccatcag
     2401 gaactgaacc tgatcccgca gttgaccatt gccgaaaaca ttttcctcgg tcgtgagttt
     2461 gttaatcgct ttggcaaaat tgactggaaa a
//
LOCUS       DAJTRC010000223         2477 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.233, whole genome shotgun sequence.
ACCESSION   DAJTRC010000223 DAJTRC010000000
VERSION     DAJTRC010000223.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.233"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(1..252)
                     /locus_tag="OO357_004101"
                     /pseudo
     CDS             complement(1..252)
                     /locus_tag="OO357_004101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(300..665)
                     /locus_tag="OO357_004102"
     CDS             complement(300..665)
                     /locus_tag="OO357_004102"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888825.1"
                     /translation="MARSAKPRKRKPAPQRSKLLRYVVKLHEDDFFDEEEAEVLRFDN
                     FDDAVECCADLNIPFFVDAGNKKLVFWFVRVDDEGYPEIARCTEREFATIPAGINADG
                     MYCPECGTVHWPDGVIPPF"
     gene            complement(1418..1891)
                     /locus_tag="OO357_004103"
                     /pseudo
     CDS             complement(1418..1891)
                     /locus_tag="OO357_004103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006708890.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase
                     domain-containing protein"
     gene            complement(1819..2185)
                     /locus_tag="OO357_004104"
                     /pseudo
     CDS             complement(1819..2185)
                     /locus_tag="OO357_004104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2187..2477)
                     /locus_tag="OO357_004105"
                     /pseudo
     CDS             complement(2187..2477)
                     /locus_tag="OO357_004105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          564 a          712 c          578 g          623 t
ORIGIN      
        1 ctcactgtct ccgttatggc gtacaccaca tcttccagta atcgccgctt caccggactc
       61 aggaccccgt atcaccatcc ggaacccctt atccgccaca tgccaggaga acctttccat
      121 aaactcgtcg ccactcatca caaggttttc ttctcgtttt gtccggtggc tgttgtaacg
      181 caggccaatt tcatcctgac cggcataatg ttccaggcgg ctcatctgca ccggctgctt
      241 ctccagccag ttaagccatc ggtttccctg ctgaaaaata tcggcaaaac ggggaagcat
      301 cagaaaggcg gaataacgcc atccggccag tgaaccgtac cgcattcagg gcaatacatg
      361 ccgtcggcgt tgataccggc aggaattgtc gcaaattccc gctccgtgca gcgggcaatt
      421 tccggatacc cttcgtcatc aacacgtaca aaccagaaga ccagtttttt gtttccggca
      481 tccacaaaga acggaatatt caggtctgcg caacactcaa cggcatcgtc aaaattatca
      541 aagcgcagaa cctcagcttc ttcttcgtca aaaaaatcat cttcgtgaag ttttacgaca
      601 tagcggagga gtttgcttct ttgaggtgca ggttttcgtt tgcgtggttt agctgaacgg
      661 gccatataac tacctgaaag acaatgatat tgtctgtttt tataacggta atcacaggcc
      721 atgacaagcc gcagccgtca ggccgcctac tcggggcggt ttagaacgct tatttagtac
      781 cagatcttac ttataataaa ggggcttggc agtttggata aactattgtc cttattccac
      841 taatacaaac tttttgtaaa gtatattaaa tatacttcat ttcatactga aatccccgga
      901 agtattcaat caaacaatta caccataaaa ggaagagggg gccccaatta agttgtcaag
      961 catgtttact gcgcaatcgc ttggtgctcc gtccctgtac cccgcattgg tactctggcg
     1021 ctcatggggc ttccacttgc gcttctccct tcacatcggg gtgacaggtc cacgttcccc
     1081 acaagagcct gaatcgactt cacgccgctt ttatgccgga tgccgcgcag gcagtaaaca
     1141 tatcccacag gaacgactcc accattgttt tgacatcatc tatatgcttt cgacacatca
     1201 tcagcggttc actttcgttc gtctcttcga accatacctg atcggctcac gtccgacctt
     1261 ttcccatctc gctcaccacc acatctctta aatgcagcag cagtggatgg tttggtgcct
     1321 gcgcctgcac gccgacacca aggggccttc cctcatctct tgtgctgcat ggctgcacag
     1381 ggactcacgt ccccaggacg cgccttcgtg gcacacagtc atcggcatag caacagtaag
     1441 caacggcagg tttccattgc cagttttctt tgacagcagt acttcggcct cgctggatac
     1501 tgtgattcca gtaccagcga tcctttctgg cttttccact caggtagcgt ttattcagat
     1561 actggtcgaa ctcattcagt atgatgttcg acagcaacgg cgagatcaca ccgccctgtg
     1621 gcatactttc actgaccgcc cggaagagac caacatcaac acggcgtttt ccacagcagt
     1681 ctcataaaac gtgtatcact gatcctgcgg cagacggctt tcatcagcag ccggtgatgt
     1741 actgtgtcga agtagctgga caggtcacct tcaataaccc ggcgccccgt gtttccccgc
     1801 agtcggtgag ctgtaatagt acgtgatgtc tcccgcccac ttctggttcg ggccactggc
     1861 gtaaaaatcc tgcgtcagca gattttctga tacaggcagg ccgtgtgcac ggtaactgat
     1921 cgggctgaac ttctgggagg cttttgcgct cagttcctga cggcgcagac tggctgccac
     1981 ggtttttacg ttaaagtggt aaccagagca cacagttcat ccgtcaggcg tggggcaccg
     2041 taacgctgga ctgggtaaaa gccttgcgga caacgctgtc gcagtgctga cggaactgcg
     2101 gacgtgtgtt tatccttgtc cgccgctgac accacgtata ccagccgctg cgggccaccc
     2161 ggagtacgcg gcacattgcc ttgatgacgg tttacgcggt cgcctgcggc aacggctttc
     2221 cggctgacgg tagtgtggac ctttttaccc cggagaacac cggcaagccc cataaccgcc
     2281 atgagacgtg ccacagtgca tctggccact ctgatacctt cccgtaacaa ctgatgccag
     2341 actttacgca cagcgtatac ctgatgattc tcatcgtata cgcgcagtat ctctttcttc
     2401 agccagtcat cgcgctgcgc acgggcactg cgtttatcat gatgatgtcg ctgttgctga
     2461 cagtggtaat acgttga
//
LOCUS       DAJTRC010000224         2474 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.234, whole genome shotgun sequence.
ACCESSION   DAJTRC010000224 DAJTRC010000000
VERSION     DAJTRC010000224.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.234"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            206..457
                     /locus_tag="OO357_004106"
     CDS             206..457
                     /locus_tag="OO357_004106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888826.1"
                     /translation="MMNIEELRKIFCEDGLYAVCVENGNIVSHYRIVCLQKNGAALIN
                     FVDARVTDGFILRDGEFVTSLQALKEIGIKAGFSAFSEE"
     gene            524..802
                     /locus_tag="OO357_004107"
                     /pseudo
     CDS             524..802
                     /locus_tag="OO357_004107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309802.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            804..1862
                     /locus_tag="OO357_004108"
     CDS             804..1862
                     /locus_tag="OO357_004108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF968 domain-containing protein"
                     /protein_id="HCR5888827.1"
                     /translation="MRVLLRPVLVPELGLVVLKPGRESMPVFHNTRVLVEPEPKSMRN
                     LPSGVVPAVRQPLVEDKTLLPFFSNARVIRAAGGAGALSDWLLRHIKSCQWPHGDYHH
                     SETVIHRYGTGAMVLCWHCDNQLRDQTSESLEQLAHQNLSAWMIDVIGHAISGTQERE
                     LSLAELSWWAVRNQVADALPEAVLRRSLGLRAEKIRSMYRESDIVPGEQTATSILKQR
                     TKNLAPLPHAHQQQNPPQEETVVSIAVDPESPAQYLQRQKPQREEMPVYTRWVKTQKC
                     MTCGNQADDPHHIIGHGLGGMGTKADDLFVIPLCRKCHNELHAGVKDFEEKHGSQLLL
                     LIRFLMHARNSGVLKWKA"
     gene            1863..2228
                     /locus_tag="OO357_004109"
     CDS             1863..2228
                     /locus_tag="OO357_004109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5888828.1"
                     /translation="MTERIEFVLPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVR
                     QQRLKLNLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDEINIVR
                     GQLVPGGRLGIKITELGCA"
     gene            2225..>2474
                     /locus_tag="OO357_004110"
     CDS             2225..>2474
                     /locus_tag="OO357_004110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5888829.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGTSIPLVSPV"
BASE COUNT          636 a          599 c          665 g          574 t
ORIGIN      
        1 gtgagaaatc cctcgccacc tctgatgtgg caggcatcct caacgcaccc gcacttaacc
       61 cgcttcggcg gttttttgtt tttattttca acgcatttga agttctggat ggtgccggaa
      121 tagaatcaaa aatacttaag tagcgcgcag ggataaaagg gatggacccc gaacagggga
      181 gtgctactta tctggaagga ttctgatgat gaacatcgaa gaactgcgta aaattttttg
      241 tgaagatggc ctctatgctg tgtgcgttga aaatggaaat attgttagtc attaccgcat
      301 tgtgtgtttg caaaaaaatg gggctgcgtt aattaatttt gtggatgccc gagtgacgga
      361 cggatttatc ttgcgcgacg gtgagtttgt cacttcatta caggcattga aagagatcgg
      421 aataaaagct ggcttttctg ctttttcaga agaataaact catctacaat cttgcgcggg
      481 gctgaactcc cgctgagtaa caccgtgcca ccggagaaaa ccgatggcac gcaacgcaaa
      541 atattacaat catgataatt cgaccgttct tgcccacacg cacgagcggt attctcacgc
      601 atttaagtca gactggtacc agcatccctc atgcactgaa gaacaggccg aatggcttat
      661 tcagtgttac cgcaggcgcg gatgcgaggt taaaaaagcc cttagcctcg actaccgtca
      721 ctagataatc tccgtcaggc tcccttactc cgaacagcca ccgcgtccgt cccgcacatt
      781 ccagcaacgg atctggaggt aacgtgcggg tattacttcg acctgttctg gttccggaac
      841 tcgggctggt ggtccttaag cccggtcgtg aatccatgcc ggtattccac aatacccggg
      901 tactggtgga gccggaaccg aaaagcatgc gtaatctgcc gtccggggtc gttcctgccg
      961 ttcgccagcc gctagtggaa gacaaaacat tgctgccgtt tttcagtaac gcacgggtga
     1021 ttcgtgctgc tggtggtgct ggcgcattgt ctgactggct gttgcgccat attaaatcct
     1081 gccagtggcc acacggcgat tatcatcaca gcgaaaccgt cattcaccgt tatggtaccg
     1141 gcgcaatggt attgtgctgg cactgcgaca accagctgcg tgaccagaca tccgaatcac
     1201 tcgagcaact tgctcatcaa aacctgtcag catggatgat tgacgtcatc ggtcacgcaa
     1261 taagcggtac gcaggagcgt gaattatctc tggctgaatt atcctggtgg gcggtccgca
     1321 atcaggtggc ggacgcgcta ccggaagcgg tattacgtcg ttcgctgggg ttgcgtgcgg
     1381 aaaaaatccg ctcaatgtac cgtgaaagcg acatcgtgcc gggagagcag accgccacca
     1441 gcatactgaa gcagcgcaca aaaaatcttg cgccgctgcc tcacgcccac cagcaacaga
     1501 atccaccaca ggaagagacg gtggtcagca ttgccgttga tccggaatct ccggctcagt
     1561 atctccagcg ccagaaacca caacgggaag agatgcctgt atacacgcgc tgggtaaaaa
     1621 cgcagaaatg catgacgtgt ggcaatcagg cagatgatcc gcaccacatc attggtcatg
     1681 gactgggagg gatgggaaca aaggctgacg atttgtttgt tattccgctg tgtcgtaaat
     1741 gccataacga actacacgcc ggggtaaaag attttgaaga aaaacacggt agtcagttgt
     1801 tgttgctgat tcgtttttta atgcacgcga gaaattcggg tgttctgaag tggaaagcat
     1861 aaatgaccga acgcatagaa tttgttttgc cttacccgcc aacggtgaac acctactggc
     1921 gacgtcgtgg cagcacatat tttgtatcaa aagccggtga gcgttatcgc cgtgatgtgg
     1981 cgctaattgt tcgccagcag cgactgaaat taaacctgtc cggaaggctg gcgataaaga
     2041 ttattgcaga gccaccggat aagcgccgtc gtgacctgga caatatcctg aaggcaccgc
     2101 tggatgcgct gacgcatgct ggactactta tagacgacga gcagtttgat gaaatcaata
     2161 ttgtgcgcgg tcagctcgtt cctggtgggc ggctggggat aaaaatcaca gaactggggt
     2221 gcgcatgaat aaccagtatt tacagtttgt gcgtgagcag ctcattatcg ccaccgctga
     2281 tttgagtggg gcaacaaaag gtcagcttga agcctggcaa gagaatgcca tgttcgatac
     2341 agggcgttac aggcgtaaaa aaatccggta ccgcgatgaa gtgactggaa aaatgataac
     2401 gcgggataat ccaccaatcc cgggaaagca atcgctggcg aaggggacgt caattcctct
     2461 ggtcagtccg gttg
//
LOCUS       DAJTRC010000225         2459 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.235, whole genome shotgun sequence.
ACCESSION   DAJTRC010000225 DAJTRC010000000
VERSION     DAJTRC010000225.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2459)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2459)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2459
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.235"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            1..264
                     /locus_tag="OO357_004111"
                     /pseudo
     CDS             1..264
                     /locus_tag="OO357_004111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416394.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
     gene            257..2334
                     /gene="flhA"
                     /locus_tag="OO357_004112"
                     /pseudo
     CDS             257..2334
                     /gene="flhA"
                     /locus_tag="OO357_004112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416393.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
     gene            2334..>2459
                     /gene="flhe"
                     /locus_tag="OO357_004113"
     CDS             2334..>2459
                     /gene="flhe"
                     /locus_tag="OO357_004113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlhE"
                     /protein_id="HCR5888830.1"
                     /translation="MRTLLAILLFPLLVQAAGEGMWQASSVGITLNHRGESMSSAP"
BASE COUNT          511 a          609 c          748 g          591 t
ORIGIN      
        1 ggtcgcgctg cgcattcgtg aaattggcgc tgaaaataac gtcccgacgc ttgaagcgcc
       61 gccgctggcg cgagcgctgt atcgacatgc ggagattggt caacaaatcc cgggtcaact
      121 gtacgccgcg gtggcggaag tgctggcctg ggtctggcaa ctgaaacgct ggcgtctggc
      181 tggtggacag cgccctgtac acctactcat cttccggtgc cggcagccct ggattttatt
      241 aacgagaaac cgacccatga gtaatctggc cgcgatgctg cgcctgcccg caaacctgaa
      301 atcgacataa tggcagatcc ttgccggacc gattttgatc ctgttgatct tgtcgatgat
      361 ggtgctgcca ctgcccgcat tcatactcga cctgttgttt accttcaata ttgccttgtc
      421 gatcatggtg ttgctggtgg cgatgtttac ccagcgcacg cttgagtttg ctgcgtttcc
      481 gaccattctg ttgtttacta tgttgttgcg tctggcactt aacgtggctt caacccgtat
      541 cattttgatg gaagggcata ccggcgcggc ggcggctggg aaggtggtcg aagcgttcgg
      601 tcacttcctc gttggtggca atttcgctat cggtatcgtg gtgtttgtca ttctcgtgat
      661 catcaacttt atggtcatta ccaaaggtgc cgggcgtata gcagaagtgg gtgcgcgctt
      721 tgttctcgat ggtatgccgg gtaagcagat ggcgattgac gccgacctta acgctggatt
      781 gattggtgaa gatgaggcga aaaaacgccg ctccgaagtg actcaggaag ccgattttta
      841 cggctcaatg gacggggcaa gtaagtttgt tcgcggcgat gccattgccg ggatcctcat
      901 catggtcatt aacgttgtcg gcgggttgct ggtcggcgtg ctgcaactgg catgagcatg
      961 ggacacgcgg cggaaagtta tacgctattg accattggcg acggtctggt ggcacaaatt
     1021 ccggcgctgg tgatttctac cgccgcgggg ggcatcgtta cgcgtgtcag caccgatcag
     1081 gatgttggcg agcagatggt gaatcagctt ttcagtaacc caagcgttat gttgttaagc
     1141 gccgccgtgc tcggtttact cggcctggtg cctggaatgc cgaacctggt atttttgctg
     1201 ttcactgccg gattgctggg gctggcctgg tggatacgcg gacgcgaaca aaaagcgcct
     1261 gccgaaccca aaccggtaaa aatggcagag aataataccg ttgtcgaagc gacgtggaac
     1321 gatgtacaac tggaagattc tctgggaatg gaagtgggtt atcgactgat cccgatggtc
     1381 gatttccagc aggatggtga gttgttgggc cgtatacgca gtatccgcaa gaaatttgcc
     1441 caggagatgg gatttctgcc gccagtggtg cacattcgcg acaatatgga tctgcaacct
     1501 gcccgctatc gcattttgat gaaaggcgtg gagattggca gtggtgatgc ttatccgggg
     1561 cgctggctgg cgattaaccc tggaaccgct gccgggacgt tacctggtga ggcgaccgtc
     1621 gatccggcat ttggcctgaa tgctatctgg attgaaagtg cgctaaaaga acaggcgcag
     1681 attcaggggt acacagtggt tgaggccagc acggtggtag caacgcatct taaccacctc
     1741 attagccagc atgccgcaga gctgtttggt cgtcaggagg cgcaacagct gttggatcgc
     1801 gtcgcccagg agatgccaaa gctgacggaa gatctcgttc ctggcgtcgt cacgctcacc
     1861 acactgcata aagtgctgca aaatctcctc gatgaaaaag taccgattcg cgatatgcgc
     1921 accattctcg aaacgctggc ggaacatgcg cccatccaaa gcgatccaca tgaattaagc
     1981 gccgtcgtgc gcgtggcgtt gggacgggcg attacccagc agtggtttcc tggcaaagat
     2041 gaagtccatg ttattggcct cgatacaccg ctggaacgtt tgttactaca ggcgctgcag
     2101 ggcgggggag gactggagcc agggctggcg gatcgtttac tggcgcaaac tcaggaagct
     2161 ctatcccgtc aggagatgct gggtgcgccg ccagtattgt tggtgaacca cgcgctgcga
     2221 ccattattgt ctcgcttcct gcgccgcagc ttgccgcagt tagtggtcct gtcgaatctg
     2281 gaactgtctg ataaccgaca tatccgcatg acggcgacaa ttggcggtaa atgatgagaa
     2341 ccttattagc gatattattg tttccgctgc tggtgcaagc cgccggggag gggatgtggc
     2401 aggcaagtag tgtcggaatt acgctaaatc atcgcggtga gtcgatgtcg tctgcacct
//
LOCUS       DAJTRC010000226         2421 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.236, whole genome shotgun sequence.
ACCESSION   DAJTRC010000226 DAJTRC010000000
VERSION     DAJTRC010000226.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.236"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..782
                     /gene="lsrF"
                     /locus_tag="OO357_004114"
     CDS             <1..782
                     /gene="lsrF"
                     /locus_tag="OO357_004114"
                     /EC_number="2.3.1.245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-hydroxy-5-phosphonooxypentane-2,4-dione
                     thiolase"
                     /protein_id="HCR5888831.1"
                     /translation="DWGMQSRLSWIFNPKTGKTVMLAFDHGYFQGPTIGLERIDINIA
                     PLFEHADVLMCTRGILRSVVPPATNKPVVLRASGANSILAELSNEAVALSMDDAVRLN
                     SCAVAAQVYIGSEYEHQSIKNIIQLVDAGMKVGMPTMAVTGVGKDMVRDQRYFSLATR
                     IAAEMGAQIIKTYYVEKGFERIVAGCPVPIVIAGGKKLPERETLEMCWQAIDQGASGV
                     DMGRNIFQSDHPVAMMKAVQAVVHHNETADRAYELYLSEKQ"
     gene            806..1096
                     /gene="lsrG"
                     /locus_tag="OO357_004115"
     CDS             806..1096
                     /gene="lsrG"
                     /locus_tag="OO357_004115"
                     /EC_number="5.3.1.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(4S)-4-hydroxy-5-phosphonooxypentane-2,3-dione
                     isomerase"
                     /protein_id="HCR5888832.1"
                     /translation="MHVTLVEINVHEDKVDEFIEVFRQNHLGSVQEEGNLRFDVLQDP
                     EVNSRFYIYEAYKDEDTVAFHKTTPHYKTCVAKLESLMTGPRKKRLFNGLMP"
     gene            1136..1894
                     /gene="tam"
                     /locus_tag="OO357_004116"
     CDS             1136..1894
                     /gene="tam"
                     /locus_tag="OO357_004116"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HCR5888833.1"
                     /translation="MSDWNPSLYLHFSAERSRPAVELLARVPLENVEYVADLGCGPGN
                     STALLHQRWPAARITGIDSSPAMIAEARSALPDCQFVEADIRNWQPEQALDLIFANAS
                     LQWLPDHYELFPHLVSLLNPQGVLAVQMPDNWLEPTHVLMREVAWEQNYPDRGREPLA
                     GVHAYYDILSEAGCEVDIWRTTYYHQMPSHQAIIDWVTATGLRPWLQDLTESEQQLFL
                     TRYHQMLEEQYPLQENGQILLAFPRLFIVARRTE"
     gene            complement(1898..>2421)
                     /locus_tag="OO357_004117"
     CDS             complement(1898..>2421)
                     /locus_tag="OO357_004117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416037.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888834.1"
                     /translation="PQAEVLAHYLKTGDLQRVLASNSPANRILLIMGEWLAVQRRNGQ
                     LSDILFISLNDRLNDISAVLAGCERIAYTPIPFAYTLILHRTVYLFCIMLPFALVVDL
                     HYMTPFISVLISYTFISLDCLAEEPEDPFGTENNDLPLDAICNAIEIDLLQMNDEAEI
                     PAKILPDRHYQLT"
BASE COUNT          618 a          560 c          657 g          586 t
ORIGIN      
        1 tggattgggg aatgcagtca cgcttatcgt ggatatttaa tccgaaaacg ggtaaaactg
       61 tgatgctggc ctttgaccat ggttattttc aggggccgac tatcggactt gaacgcattg
      121 atataaatat cgccccgctg tttgaacatg ccgatgtatt aatgtgtacg cgcggcattt
      181 tgcgcagcgt agttcccccg gcgaccaata agccggtggt actgcgggcg tcaggtgcga
      241 actctattct ggcggaatta agtaatgaag ccgtggcgtt atcgatggat gatgccgtgc
      301 gcctgaacag ttgcgcggtg gcggcgcagg tttatatcgg cagcgaatat gaacatcagt
      361 cgatcaaaaa tattattcag ctggtcgatg ccggaatgaa agtgggaatg ccgaccatgg
      421 ccgtgactgg cgtgggcaaa gatatggtgc gcgatcagcg ttatttctcg ctcgcgactc
      481 gaatcgccgc tgaaatgggg gcgcaaatta tcaaaaccta ttatgtcgaa aaaggttttg
      541 aacggattgt tgccggatgt ccggtaccca ttgttattgc tggcggtaaa aaattaccgg
      601 agcgcgagac gctggaaatg tgctggcagg ctatcgatca gggcgcttct ggtgtggata
      661 tggggcgtaa tattttccag tctgaccatc cggtggcgat gatgaaagcc gtacaggcgg
      721 tggttcacca taacgaaacg gctgatcggg catatgaact ctatctgagt gaaaaacagt
      781 aactgcggat ttaaggagaa gaattatgca cgtcacactg gttgaaatta acgttcatga
      841 agacaaggtt gacgagttta tcgaagtttt tcgccagaac cacctgggct ctgtacagga
      901 agaaggtaat ttgcgcttcg atgtcttaca ggacccggaa gtgaattcgc gcttttatat
      961 ttacgaagcc tataaagatg aagacacagt ggcattccat aaaaccacgc ctcactacaa
     1021 aacctgtgtc gcgaaactgg aatctttaat gaccgggccg cgtaaaaaac gtctgttcaa
     1081 tgggttgatg ccgtgaggct acaattgggg taacgcgctg acgggagtaa aaaaaatgtc
     1141 tgactggaac ccctctttat atctgcactt ttccgctgaa cgatcgcgtc cggcggtgga
     1201 gctgcttgcc agagtgccgc tggagaatgt cgaatatgtt gccgatcttg gctgtggccc
     1261 aggtaacagc accgccctgc tgcatcaacg ttggcctgcg gccaggataa caggcatcga
     1321 ctcgtctccg gcgatgattg ctgaagcgcg cagtgctttg ccagactgcc agttcgtgga
     1381 agcagacatc cgcaactggc aaccggaaca ggcactcgat ctgatttttg ctaatgcctc
     1441 actgcaatgg ctgcccgacc actacgaatt gtttcctcac ttggtttctt tacttaatcc
     1501 gcagggcgtg ctggcagtac agatgccaga taactggctg gagccgaccc atgtgctcat
     1561 gcgcgaagtt gcttgggaac aaaactaccc ggatcgcggg cgtgagccgt tggctggcgt
     1621 tcatgcttac tacgatattt tgagcgaagc cggatgtgag gtcgatatct ggcgaaccac
     1681 ttactatcac cagatgccgt cgcaccaggc gattatcgat tgggtgaccg ccactggatt
     1741 acgtccgtgg ttacaggatc tgaccgagag cgaacagcag ctttttctta cgcgctacca
     1801 tcagatgctg gaagagcagt atccactgca agagaacgga cagatactgc tggcatttcc
     1861 gcgtctgttt attgttgccc gccgtacgga gtaattatca cgtcagctgg taatgacgat
     1921 cgggaagaat ttttgctgga atttctgctt catcgttcat ctgtaacagg tcaatttcaa
     1981 tagcgttgca gatggcatcc agcggtaaat cattgttttc agtaccgaac ggatcttccg
     2041 gttcttccgc cagacaatcc agcgaaataa aagtgtagga aatcagcaca gagataaaag
     2101 gcgtcatgta atgcaggtcc acgaccagcg cgaacggcag catgatacaa aacagataaa
     2161 cggtacgatg caaaatcagg gtgtaggcaa agggaattgg cgtgtaggca atgcgctcac
     2221 agcctgccag aaccgctgaa atatcattaa gccgatcgtt gaggctaata aacaggatat
     2281 ctgaaagctg tccattgcgg cgctgaaccg ccaaccattc tcccattatt aacaagatac
     2341 ggttagctgg agagttcgaa gccagtacac gctgaagatc tccagtcttg agataatgag
     2401 ccagcacttc cgcctgtggc t
//
LOCUS       DAJTRC010000227         2420 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.237, whole genome shotgun sequence.
ACCESSION   DAJTRC010000227 DAJTRC010000000
VERSION     DAJTRC010000227.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2420)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.237"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..201
                     /locus_tag="OO357_004118"
     CDS             <1..201
                     /locus_tag="OO357_004118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888835.1"
                     /translation="KLKMMLFGASLIMVVGCSSKENALCHPQPKPPAPPAWAMMPPSN
                     SLQLLDETFSVSGTESSATRQH"
     gene            complement(334..846)
                     /locus_tag="OO357_004119"
     CDS             complement(334..846)
                     /locus_tag="OO357_004119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HCR5888836.1"
                     /translation="MMEWINALMNYFVLHPYRLWGLLFIIAFTKSTLLISAILPPASI
                     MMATVITVSKTTLSPWEAGITVMSGAWCGTIVNYHLGIIIGHIPQLACIISSRSNTIE
                     RVRLQLQNNSVSILFTSRFIAVLRYIAPLVAGMLQLHPVKVYTVSLISAASWSALYVG
                     SFSFVLPFFS"
     gene            1041..1805
                     /locus_tag="OO357_004120"
     CDS             1041..1805
                     /locus_tag="OO357_004120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888837.1"
                     /translation="MAKPDWEAIESAYRAGVLSLRDIGEKYGVTEGAIRKRAKKLDWA
                     RSGGTQVCKNGTQKRKVRTSRKPAITGLTQKSTQLKTESTPDTKPIRGMRTDPPTNPF
                     QPGNQQALKHGGYARRLLLKDEVIEDAKALTLEDELFRLRANNLVAAENIGRWLVSLE
                     DANGDQERKMLIENISAAEKAMMRNTVRIESIVGTLATVGKIFADTAYRKAATDKVSL
                     EADRLRRDAGIDDGNGERDLNDFYSDIQTDAESGFT"
     gene            1756..>2420
                     /locus_tag="OO357_004121"
     CDS             1756..>2420
                     /locus_tag="OO357_004121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005226564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HCR5888838.1"
                     /translation="MTSTLTSKPTLNPALRSFWTMRARNKVLYGGRSSSKSWDAAGIA
                     IFLSNKYTLRFCCARQIQNKIEESVYTLLKIQIDRFGLRHRFRILNNKIINRVTGSEF
                     VFYGLWRNIEEIKSLEGIDMLWLEEAHALTEYQWKILEPTIRKEGSECWFIFNPGLVT
                     DFVWRNFVVDPPEGTLIRKINYDENPFLSDTMLKVIDAARRRDPDGFKHVYEGVPESD
                     DDAA"
BASE COUNT          682 a          540 c          614 g          584 t
ORIGIN      
        1 aagctgaaaa tgatgctctt cggcgcaagc ttgataatgg tggtcgggtg ctcgtcaaag
       61 gaaaatgccc tgtgccatcc tcagccgaaa cctccagcgc ctccggcatg ggcaatgatg
      121 ccaccgtcga actctctcca gttgctggac gaaacgttct cggtatccgg gacggaatca
      181 tcagcgacca gacagcattg agaatgcttc aggagtacat caggatccaa tgccttgggg
      241 ggtagcggta attttactca ttatccttca aatcagattc tgttgtcaga ggaatggggg
      301 acgctggatt cgcaagagct ttttatgggc agtttagcta aaaaaaggca acacgaagct
      361 aaaactacca acataaagtg ctgaccagct agcagctgaa atcagactca ctgtatacac
      421 tttcactgga tgaagttgca acattcctgc caccaaagga gcaatgtaac gcagcacggc
      481 gataaagcgc gaagtgaaaa gtattgacac agagttattt tgcaactgaa ggcgaacccg
      541 ttctattgta ttggaacggc tcgatattat acatgcaagt tgagggatat gcccaattat
      601 tataccaaga tggtaattaa cgatagtacc gcaccatgcg ccactcatga ccgtgattcc
      661 agcttcccat ggtgaaagag tcgtcttgct gactgtaatt acagtggcca tcataattga
      721 tgctggtggc aatatcgccg aaatgagtaa agtggatttt gtgaacgcta ttataaaaag
      781 aagcccccat aggcgatatg gatgaagtac aaaatagttc atcaaagcat ttatccactc
      841 catcaatgtc tttccttctg tgaatttttc tttgctattt aaccgtgata cgacttaatt
      901 taggcttaaa caccttacat ttacaaggta ctcctgtggg gggccctgcc acggagcgtc
      961 gcaggagtga gtgatgagga agaattaaag caacctattg tgacaggcag cgataacagt
     1021 aaatcacagg aagcataatc atggcaaaac cggactggga ggccatcgaa tcggcatacc
     1081 gggccggagt ccttagcctc cgtgatatag gcgagaaata cggcgttaca gaaggggcta
     1141 tcaggaagag ggccaaaaag cttgattggg cacgcagtgg cggtacgcag gtttgcaaaa
     1201 atggtacgca aaaaaggaaa gtgcgtacca gcagaaagcc tgccattact ggccttacac
     1261 aaaaaagtac gcaactaaaa acagaatcta caccggatac gaaaccgata cgcggaatgc
     1321 gtaccgatcc cccgactaac ccattccaac ccggtaacca gcaggcatta aaacacggtg
     1381 gttatgcccg tcgccttctg ctcaaagatg aggtgataga ggacgctaaa gcgttgacgc
     1441 tcgaggacga attatttcgc cttcgtgcta acaaccttgt cgctgcagag aatattggtc
     1501 ggtggttggt gtcgctggaa gatgctaatg gggaccagga aaggaagatg ctgattgaaa
     1561 atatcagcgc cgccgagaaa gcaatgatgc gcaatacagt tcgtattgag tccatcgttg
     1621 gcacgcttgc gacggtagga aaaatatttg ctgatacagc ctaccgcaag gccgccactg
     1681 ataaggtgtc tctggaggct gatcgtcttc gccgtgatgc aggtattgat gatggcaatg
     1741 gagagcgtga cctcaatgac ttctactctg acatccaaac cgacgctgaa tccggcttta
     1801 cgtagtttct ggactatgcg ggcacgtaac aaagtgcttt atggtggtcg gtcatcgtca
     1861 aaatcatggg atgccgctgg cattgccata tttctgtcga ataaatacac cctgcgtttt
     1921 tgttgtgccc gtcagatcca gaataaaatc gaagagtcgg tgtataccct gctcaaaatt
     1981 cagatagaca ggtttggtct gcggcaccgt ttccgtattc tgaacaacaa aatcattaac
     2041 cgggttactg gctcggaatt tgttttttat ggattatggc gcaacatcga agaaattaag
     2101 tcactggagg ggatcgatat gttgtggttg gaagaagccc acgcactgac ggaataccag
     2161 tggaaaattc tggagccaac gatccgtaaa gagggttcgg aatgctggtt catattcaac
     2221 cccggacttg ttactgattt cgtctggcgc aacttcgttg ttgatccgcc cgaaggcact
     2281 ctcatccgca aaattaacta tgacgaaaat ccgtttctgt ctgacaccat gcttaaggtt
     2341 atcgacgcgg cgcgacgccg tgatccggat ggttttaaac atgtgtatga gggcgttccg
     2401 gagtctgatg atgatgcggc
//
LOCUS       DAJTRC010000228         2382 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.238, whole genome shotgun sequence.
ACCESSION   DAJTRC010000228 DAJTRC010000000
VERSION     DAJTRC010000228.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.238"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(64..822)
                     /locus_tag="OO357_004122"
     CDS             complement(64..822)
                     /locus_tag="OO357_004122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HCR5888839.1"
                     /translation="MSQILLQPANAMITLENVHKWYGQFHVLKNINLTVQPGERIVLC
                     GPSGSGKSTTIRCINHLEEHQQGRIVVDGIELNEDIRNIERVRQEVGMVFQHFNLFPH
                     LTVLQNCTLAPIWVRKMPKKEAEALAMHYLERVRIAEHAHKFPGQISGGQQQRVAIAR
                     SLCMKPKIMLFDEPTSALDPEMVKEVLDTMIGLAQSGMTMLCVTHEMGFARTVADRVI
                     FMDRGEIVEQATPDEFFAHPKSERTRAFLSQVIH"
     gene            complement(830..1933)
                     /locus_tag="OO357_004123"
     CDS             complement(830..1933)
                     /locus_tag="OO357_004123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709065.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HCR5888840.1"
                     /translation="MTKVLLSQPSRPASHNSSRAMVWVRKNLFSSWSNSLLTIGCIWL
                     MWELIPPLLNWAFLQANWVGSTRADCTKAGACWVFIHERFGQFMYGLYPHDQRWRINL
                     ALLIGLVSIAPMFWKILPHRGRYIAAWAVIYPLIVWWLMYGGFFGLERVETRQWGGLT
                     LTLIIASVGIAGALPWGILLALGRRSHMPIVRILSVIFIEFWRGVPLITVLFMSSVML
                     PLFMAEGTSIDKLIRALVGVILFQSAYVAEVVRGGLQALPKGQYEAAESLALGYWKTQ
                     GLVILPQALKLVIPGLVNTIIALFKDTSLVIIIGLFDLFSSVQQATVDPAWLGMSTEG
                     YVFAALIYWIFCFSMSRYSQHLEKRFNTGRTPH"
     gene            complement(1943..>2382)
                     /locus_tag="OO357_004124"
     CDS             complement(1943..>2382)
                     /locus_tag="OO357_004124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312169.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5888841.1"
                     /translation="FNFRGGMVLIPELAALTLALSVYTSAFIAEIIRAGIQAVPYGQH
                     EAARSLGLPNPVTLRQVIIPQALRVIIPPLTSQYLNIVKNSSLAAVIGYPDMVSLFAG
                     TVLNQTGQAIETIAMTMSVYLIISLTISLLMNIYNRRIAIVER"
BASE COUNT          654 a          638 c          551 g          539 t
ORIGIN      
        1 catcaaatca agcgaaaggc catccgaaag gatggccttt ttgcttttcg aactaacatt
       61 caattaatgg attacctgcg ataaaaatgc cctcgtacgc tctgatttag gatgcgcaaa
      121 aaattcatca ggtgtagctt gttccactat ctccccacga tccataaaaa ttacccggtc
      181 agcgacggta cgtgcaaacc ccatctcatg tgttacacac aacattgtca tacccgactg
      241 cgccagccca atcatcgtat ccagcacctc tttcaccatc tccggatcga gcgccgacgt
      301 tggttcatca aacaacataa ttttcggctt catacacagc gaacgcgcaa tggcaacgcg
      361 ttgctgctga ccacctgaaa tctgtccggg aaacttatgc gcatgttcgg caattctcac
      421 ccgctcaagg taatgcatcg ccagagcttc agcctctttc ttaggcatct tgcgtaccca
      481 aatcggtgcc agggtacagt tctgtaaaac ggtcagatga gggaagagat tgaaatgctg
      541 aaataccatt cccacttcct gcctgacgcg ctcaatattg cggatatctt cattaagttc
      601 gatgccatct acgacaatcc gtccctgttg atgttcttcc agatgattaa tacaacgaat
      661 ggttgtcgat ttaccggaac cagaagggcc acacagaacg atccgttctc ccggttgcac
      721 ggttaaattt atatttttca aaacatggaa ttgtccatac catttatgga cgttttccag
      781 cgtaatcatc gcgttagcag gttgcagtaa aatttggctc atagtgtcct caatgcggtg
      841 tacgcccggt gttaaaacgt ttttccagat gttggctata acgtgacatg ctgaaacaaa
      901 agatccagta gatcagcgcg gcgaaaacat acccttccgt cgacataccc aaccaggcgg
      961 gatcaacggt tgcctgctga acgctactga aaagatcgaa caagccgatg atgatcacta
     1021 ggctggtatc tttgaagagt gcgatgatgg tatttaccag cccaggaatt accagcttca
     1081 acgcctgtgg cagaataacc agcccctgag ttttccagta acccaacgcc agcgactctg
     1141 ccgcttcata ttgcccttta ggcagcgcct gtaatccacc tcgcacgact tccgcaacat
     1201 atgctgactg aaacaggatc acgccaacca gcgcgcggat cagtttgtcg atactggtgc
     1261 cttctgccat aaacaacggc agcatgaccg aagacataaa caatacggta atcagcggta
     1321 cgccgcgcca gaactcgata aaaatgaccg ataagatacg cacaatcggc atatgggagc
     1381 ggcgacctaa tgccagtaag atcccccacg gtagcgcccc cgcaatccca acggatgcaa
     1441 taattaaagt tagcgtcagc ccgccccatt gccgggtttc aacccgctca agaccaaaaa
     1501 aaccgccata catcagccac cagacaatca gtgggtaaat caccgcccag gccgcaatat
     1561 agcgaccgcg atgcgggagt attttccaga acattggtgc gatcgataca agcccaatca
     1621 gtaatgcgag gttaattcgc cagcgttggt cgtgtgggta aagtccatac ataaactgac
     1681 caaatcgttc gtgaatgaag acccaacagg caccggcttt tgtgcagtca gcacgcgttg
     1741 agccaaccca attagcctgc aaaaatgccc agttcagcaa cggtgggatc agttcccaca
     1801 tcaaccatat gcagccaata gtcagcaggc tattgctcca gctggagaac agattttttc
     1861 gcacccacac catcgcgcgg cttgagttat ggctcgccgg gcgcgagggc tgagacagca
     1921 atacttttgt catcatgact ccttagcgtt caacgatcgc gatgcggcgg ttatagatat
     1981 tcatcagcag cgagatagtc aggctgataa tcagatagac cgacatggtc atggctatcg
     2041 tctcgatggc ttgccccgtc tggttcagca cggtgccagc aaacagcgaa accatatcgg
     2101 gatagccaat aacggcggca agagaggagt ttttgacgat gttgagatac tggctggtta
     2161 acggcggaat aattactcgc agtgcctggg gaataatgac ctggcgtagc gtaaccgggt
     2221 tgggtaaccc cagtgaccga gccgcttcat gttgaccata aggcactgcc tggatcccag
     2281 cgcggataat ctcggcgata aatgcagagg tataaaccga aagtgccagc gttaaggctg
     2341 ccagttcagg aattaaaacc attccgccgc ggaagttaaa gc
//
LOCUS       DAJTRC010000229         2269 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.239, whole genome shotgun sequence.
ACCESSION   DAJTRC010000229 DAJTRC010000000
VERSION     DAJTRC010000229.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.239"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(39..194)
                     /locus_tag="OO357_004125"
     CDS             complement(39..194)
                     /locus_tag="OO357_004125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706802.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1391 family protein"
                     /protein_id="HCR5888842.1"
                     /translation="MNTIDLGNNESPVYGVFPNQDGTFTAITYTKSKTFKTEAGARRW
                     LGRHSGE"
     gene            complement(397..804)
                     /locus_tag="OO357_004126"
     CDS             complement(397..804)
                     /locus_tag="OO357_004126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5888843.1"
                     /translation="MKNETFGARLLHRRKKLKLSQAALGKLVKVAHVTISQWERDETQ
                     PAGKRLFALSQALQCSPTWLLFGDEDKQPGEPIPDNQPAILTEDQKELLQLFDALPES
                     EQKALLSEMRARVENFNKLFEELLKARKRSANK"
     gene            881..1108
                     /locus_tag="OO357_004127"
     CDS             881..1108
                     /locus_tag="OO357_004127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5888844.1"
                     /translation="MLKIDAIAFFGSKTKLANAAGVRLASIAAWGELVPEGRAMRLQE
                     ASGGELQYDPKVYDEYRKAKRPGKVIHENQA"
     gene            1092..1514
                     /locus_tag="OO357_004128"
     CDS             1092..1514
                     /locus_tag="OO357_004128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888845.1"
                     /translation="MKIRHEHIESVLLALAAEKGQAWVANAITEEYLRQGGGELSLVP
                     GKDWNNQQNIYHRWLKGETKAQREKIQKLIPAVLAILPRELRHRLCIFDTLERRALLA
                     AQEALSTAIDAHDDAVQAVYRKAHFSGGGSPGDSVVVH"
     gene            1592..2230
                     /locus_tag="OO357_004129"
     CDS             1592..2230
                     /locus_tag="OO357_004129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888846.1"
                     /translation="MASNWIKLEVITPDKPEIFRLAEILNIDPDAALGKVIRFWAWAD
                     QQMIDGNADCNARGVTKSAIDRITFMSGFADALIQVGWLVENDGGLSLPNFERHNGKS
                     SKKRAVTNERVTKIRELKRKGNAASVTQTDQKALPVEEEEEDLNTDLPLNPPRQKRAS
                     KKFEPEAIELPDWLPETLWHEWVRFRQALRKPIRTEQGDTGTGKIPSAGFYT"
BASE COUNT          603 a          470 c          596 g          600 t
ORIGIN      
        1 gtagagcgat aacgtgtttt aattctgtct cgttcatttt actcacctga atgtcttccc
       61 aaccaacgac gtgcgccagc ttcggtttta aacgttttgc ttttggtata cgttatggcg
      121 gtgaatgtgc cgtcctggtt ggggaacacg ccgtacaccg gagattcgtt gttgccaaga
      181 tcgatagtat tcatgttgac cccatttccc cttaacgccg gggtagcgga actgtttgct
      241 gagaacaccg tgcggtgtct tgatgaataa aatttagaat aacctaagga gggtgatcaa
      301 gatttttgtg tagaaaaacc taagtttttt gatgtaaaaa acacaagtgt ttgaaagttt
      361 gtgcttttta ttacagggtg tggagaaaaa aggggattat ttgtttgcgc ttcttttgcg
      421 agctttgagt agttcttcaa aaagtttgtt gaaattttca actcgagcac gcatctctga
      481 caacagggcc ttttgctctg actcaggcag tgcgtcgaac agttgaagta actctttttg
      541 atcttctgtc agaatggctg gctgattatc cgggatcggt tcgcctggtt gtttatcttc
      601 atccccaaaa agaagccaag tcggtgagca ctgaagcgct tggctcagtg cgaataatct
      661 tttccccgcc ggctgtgttt catctctttc ccattgagaa attgttacgt gagccacttt
      721 gaccagctta cctaatgcgg cctgagacag ttttaatttt ttacgcctat gtaagaggcg
      781 agcaccgaag gtttcgtttt tcatattagg gaattctaat ttttcttgac ttaggtttct
      841 ctacggcctg gtttccttag gaaaatctaa ggagctcgat atgttgaaaa ttgatgctat
      901 agcgtttttt ggcagcaaaa caaagcttgc caatgccgca ggagttaggc tggcaagcat
      961 tgctgcatgg ggggaactgg ttcctgaagg tcgcgcgatg cgcctgcaag aggcatctgg
     1021 cggggaactt cagtacgacc ccaaagttta tgacgaatat cgtaaggcaa aacgacctgg
     1081 gaaggtgatt catgaaaatc aggcatgagc acatcgaatc agtgctgtta gccctggcag
     1141 ccgaaaaagg gcaggcgtgg gtcgctaacg caattactga agaatatctg cggcaggggg
     1201 gcggcgaatt gtctctggta ccaggcaagg actggaacaa tcagcagaat atctatcacc
     1261 gttggttgaa aggtgaaacg aaagcgcaaa gggaaaaaat tcagaaactg atccctgcgg
     1321 ttctggcaat tcttccgcgt gagctgcgtc accgactctg tatcttcgat accctggaac
     1381 gccgtgcatt actggcggcg caggaagcgt tgagtacggc aattgatgcg catgatgatg
     1441 ctgtccaggc cgtttaccgg aaagcacatt tcagcggtgg tgggtcgccc ggcgattctg
     1501 tcgtagtgca ttgattgaaa ttaatcgtgc cggattgttt tgttcggtat cagttaaatg
     1561 taacgctgcg agcgttacaa ggtgaaaaca aatggcttca aactggataa agctcgaggt
     1621 tattacgccg gataagccgg aaatattcag gcttgctgag attctgaata ttgatccaga
     1681 tgccgcatta gggaaggtta ttcgcttctg ggcatgggcg gatcaacaaa tgatagacgg
     1741 taatgcagat tgtaacgctc gcggcgttac aaaaagtgca atagatcgca tcacttttat
     1801 gtctggtttt gctgatgcgt taattcaggt tggatggctg gtcgaaaatg acggtgggct
     1861 ttctctacct aactttgaac gtcataacgg aaaaagctct aaaaaacggg cggttacaaa
     1921 cgagcgagta acaaaaatac gcgaactgaa acgaaaaggt aacgctgcca gcgttacaca
     1981 aacggatcaa aaagcgttac cagtggaaga ggaagaggaa gatctaaata ctgatctccc
     2041 cctaaatccc cctcgccaaa aacgagcgtc taaaaaattc gagccggagg ctattgagct
     2101 gcccgattgg ttgccggaaa cactctggca tgagtgggtc cggttcagac aggcattgcg
     2161 aaaaccgatt cgaacggagc agggcgatac gggaactgga aaaattccgt cagcagggtt
     2221 ttacacctga gcaggtgatt cgacacagca tcgccaatga ataccaggg
//
LOCUS       DAJTRC010000230         2260 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.240, whole genome shotgun sequence.
ACCESSION   DAJTRC010000230 DAJTRC010000000
VERSION     DAJTRC010000230.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2260)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2260)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2260
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.240"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            671..2179
                     /gene="ltrA"
                     /locus_tag="OO357_004130"
     CDS             671..2179
                     /gene="ltrA"
                     /locus_tag="OO357_004130"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HCR5888847.1"
                     /translation="MQRKLATWAATDPSLRIQRLLRLITQPEWLAEAARITLSSKGAH
                     TPGVDGVNKTMLQARLAVELQILRDELLSGHYQPLPARRVYIPKSNGKLRPLGIPALR
                     DRIVQRAMLMAMEPIWESDFHTLSYGFRPERSVHHAIRTVKLQLTDCGETRGRWVIEG
                     DLSSYFDTVHHRLLMKAVRRRISDARFMTLLWKTIKAGHIDVGLFRAASEGVPQGGVI
                     SPLLSNIMLNEFDQYLHERYLSGKARKDRWYWNNSIQRGRSTAVRENWQWKPAVAYCR
                     YADDFVLIVKGTKAQAEAIREECRGVLEGSLKLRLNMDKTKITHVNDGFIFLGHRIIR
                     KRSRYGEMRVVSTIPQEKARNFAASLTALLSGNYSESKVDMAEQLNRKLKGWAMFYQF
                     VDFKAKVFSYIDRVVFWKLAHWLARKYRTGIASLMRWWCKSPKPGQSKTWVLFGKTNH
                     GKLSGEILYRLVGQGKKLFRWRLPEGNPYLRTETRNTYTSRFTEVAMAFASI"
BASE COUNT          574 a          559 c          668 g          459 t
ORIGIN      
        1 tgtgcccgga gttcagggcg ggcatggatg cttaaatgaa ccgcgagtct gtctggaata
       61 ttgaaccggt aactcacgat gagaaaccca acaatcccac cgggtgtgac ggtggagaac
      121 ctgagcggca gtgacctgcg gcatgcccgc agggtgatgt aacccgctga caacggggat
      181 tgaggcgaga tcactaagcc gagatgatcc tcaaggttaa gtactgaaag gctgaagaac
      241 atgaacccgt taatccgcct ctgtgggttg aaaacgtcac cacggcctac gtgatctgac
      301 aggccgtgca ggaggaactg gcagtgatac gtaagcactg ccggtcgaag gtgttttgac
      361 atgtatgcga aacaccgggg cagcagcgtc tatcacactt gcgttgctga cttctgccaa
      421 cttgcggcaa gcaaggataa agagtgcgac gggcagcctc ctcagtatgc ctgagtccag
      481 gcaggtaaac cggggaaggt cagcgacgga tgttaagggg gcatggctcc gatgacgcgc
      541 tggctggcgg agcttccgta gtagtccgcg atggggaaag cccattacat ggcgaaggga
      601 agcagtttga atgtgtttgc gacgtgaatt aactgaccta acgaggtgaa gacctttgat
      661 aatcagcgaa atgcaacgca agcttgccac atgggcagcc accgatccgt ccctacggat
      721 tcaacggctg ctgcgtctga taacacaacc agaatggctg gctgaagcgg cgcggatcac
      781 gctttcatca aagggggccc atacccccgg cgttgatggc gtgaacaaaa caatgctaca
      841 ggccagactg gctgttgagc tgcaaatcct cagggatgaa ttactctcag gccactacca
      901 gcccttgccc gccagacggg tttacatccc taaaagcaac ggcaaactgc gaccactggg
      961 tatccccgcg ttgcgggatc gtattgttca gcgggccatg ctgatggcga tggagccgat
     1021 atgggagagt gattttcata cgctctcata tggcttccgg cctgagcgca gtgtccacca
     1081 cgcgatccgc acggtgaaat tacagctcac agactgtggt gaaacccggg gacgctgggt
     1141 gattgaaggc gacctgtcca gttacttcga caccgtacat catcgactgc tgatgaaggc
     1201 tgtacgccgc aggatcagtg acgcacgttt catgactctg ctgtggaaaa ccatcaaggc
     1261 gggacatatc gatgtcggtc tctttcgggc ggccagtgaa ggtgtgccac agggcggtgt
     1321 tatatcgccg ctattatcga acatcatgct gaatgagttc gatcaatacc tgcatgagcg
     1381 ctacctgagc gggaaagcca gaaaagatcg gtggtactgg aataacagta tccaacgggg
     1441 ccgaagtacg gcggtcagag aaaactggca gtggaaaccc gcggtggcgt actgccgcta
     1501 tgccgatgat tttgtcctca tcgtcaaagg caccaaagca caggcggaag ccatcaggga
     1561 ggagtgtcgg ggtgtgctcg aaggcagtct gaaactcagg ctgaacatgg ataagactaa
     1621 aatcacccat gttaatgacg gctttatctt tctggggcac aggatcattc gcaaacgcag
     1681 tcgttatggc gagatgcgag tggtctcaac gatcccgcag gagaaagcca gaaacttcgc
     1741 cgcatcgctg acagcactgt tatcaggcaa ctacagtgaa agcaaagtcg atatggctga
     1801 acaactcaac cgaaaactga aaggctgggc catgttctat cagttcgttg attttaaggc
     1861 caaagtcttc agttatatcg accgtgtcgt gttctggaag ctggctcact ggctggcccg
     1921 caaataccgt acaggtatcg cttccctgat gaggtggtgg tgtaaatcac cgaaaccggg
     1981 tcagagcaaa acgtgggttt tatttggtaa aaccaatcac ggcaagctca gcggcgaaat
     2041 actgtaccgg ttggtggggc aaggcaagaa gctgttccgc tggcggctac ccgaaggtaa
     2101 tccctatctg aggacggaga ccagaaacac gtatacatcg cgctttacag aagtggcaat
     2161 ggcgttcgcc agcatttaaa tggagagccg gatgcgctga aaggtgcacg tccggttcgg
     2221 ggaggagagg cagggaaata gtccgactac gccctgcctc
//
LOCUS       DAJTRC010000231         2141 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.241, whole genome shotgun sequence.
ACCESSION   DAJTRC010000231 DAJTRC010000000
VERSION     DAJTRC010000231.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2141)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2141)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2141
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.241"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..371
                     /locus_tag="OO357_004131"
     CDS             <1..371
                     /locus_tag="OO357_004131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5888848.1"
                     /translation="PNFPYEFKIALVEQSLQPGACVAQIARENGINDNLLFNWRHQYR
                     KGGLLPSGKNMPALLPVTLTPEPDNKIPAPAQEPEQINTPSDSLCCELVLPAGTLRLK
                     GKLTPALLQILIREIKGSSH"
     gene            371..718
                     /gene="tnpB"
                     /locus_tag="OO357_004132"
     CDS             371..718
                     /gene="tnpB"
                     /locus_tag="OO357_004132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5888849.1"
                     /translation="MISFPAGSRIWLVAGITDMRNGFNGLVSKVQNVLKDDPFSGHLF
                     IFRGRRGDQIKVLWADSDGLCLFTRRLERGRFVWPVTRDGKVHLTPAQLSMLLEGIDW
                     KHPKRTERAGIRI"
     gene            738..1967
                     /locus_tag="OO357_004133"
                     /pseudo
     CDS             738..1967
                     /locus_tag="OO357_004133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            1971..2141
                     /locus_tag="OO357_004134"
                     /pseudo
     CDS             1971..2141
                     /locus_tag="OO357_004134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          516 a          579 c          601 g          445 t
ORIGIN      
        1 ggcctaattt tccttatgaa tttaaaatcg ctttagtgga gcagtcactg cagcccggag
       61 cctgtgtggc gcagatcgcc cgtgaaaacg gaatcaacga taacctgctc ttcaactggc
      121 gccatcaata ccggaaaggt ggcctgctgc cttccggaaa aaatatgcct gcactgcttc
      181 ccgtgacgtt aacgccggag ccggataata aaatcccggc tccggcacag gaaccagagc
      241 agataaatac accgtccgac agtctgtgct gtgagctggt tctgccggcc ggaactctca
      301 ggcttaaagg taaactgacg ccggcgttat tacagatact tatccgcgaa ataaaaggga
      361 gcagccactg atgatatctt tccctgcagg ttcgcgtatc tggctggttg caggtatcac
      421 cgatatgcga aatggcttta acggcctggt ctcaaaagtt cagaacgtcc tgaaggatga
      481 cccgttctcc gggcatctgt tcatcttccg cggacgccgg ggtgaccaga taaaagtgtt
      541 gtgggctgac agtgacggac tgtgcctctt taccagacgc ctggagcggg gccgcttcgt
      601 ctggccggtc acccgtgacg gcaaggtgca ccttactccg gcccagttat ccatgcttct
      661 tgagggcatc gactggaaac acccgaaacg aacggaacgc gctggcatcc gcatataacc
      721 cgttgtaaag tgaggatatg gacacctcac ttgctcatga gaatgcccgc ctgcgggcac
      781 tgttgcagac gcaacaggac accatccgcc agatggcgaa atacaaccgc ctgctctcac
      841 agcgggtggc ggcttatgct tccgaaatca accggctgaa ggcgctggtt gcgaaactgc
      901 agcgcatgca gttcggtaaa agctcagaaa aacttcgcgc aaaaaccgaa cggcagatac
      961 tggaagcaca ggagcgaatc agcgcacttc aggaagaaat ggcggaaacg ctgggtgagc
     1021 aatatgaccc ggtactgcca tctcccctgc gccagtcttc agcccgtaaa ccgttaccag
     1081 cctcacttcc ccgtgaaacc cgggtcatcc gtccggaaga ggaatgctgc ccggcctgtg
     1141 gtggcgaact cagttctctg ggatgtgatg tgtcagagca gctggagctt atcagcagcg
     1201 cctttaaggt tatcgaaaca caacgtccga aactggcctg ttgccggtgt gaccatatcg
     1261 tgcaggcacc ggtgccttca aaacccattg cacgcagtta tgccggagcg gggcttctgg
     1321 cccatgttgt tgctggaaaa tatgcagact atctgccgtt ataccgccag tcagaaatat
     1381 accgtcgtca gggcgtggag ctgagccgtg ccacactggg gcgctggaca ggtgctgttg
     1441 ctgaactgct ggaaccgctg tatgacatcc tgcgccagta tgtgctgatg cccggtaaag
     1501 tccatgccga tgatatcccc gttccggtcc aggagccggg cagcggtaaa acaaggactg
     1561 cccggctgtg ggtctacgtt cgtgatgacc gtaacgccgg ttcggaaatg cctccggcgg
     1621 tctggttcgc gtactcaccg gaccggaaag gtatccatcc acagaatcat ctggccggtt
     1681 acagcggagt gcttcaggcc gatgcttacg gtggttaccg ggcgttgtac gaatccggca
     1741 gaataacgga agccgcatgt atggctcatg tccggagaaa aatccacgat gtgcatgcaa
     1801 gagtgccaac cgacatcacc acggaagccc tgcagcgtat cggtgaactg tatgccatcg
     1861 aggcagaagt ccggggatgt tcagcagaac agcgtctggc ggcaagaaaa gccagagccg
     1921 cgccactgat gcagtcactg tatgactgga tacagcaaca gatgaaaatt cattcgctga
     1981 aaatggaatg tctccacgga gaacactatt atccatcggg aaatagtgcg ggcaacagtg
     2041 tttagttata tcgagtgcga ttacaatcgc tggcgacggc accgttggtg tggtggtctc
     2101 agtccggaac aatttgaaaa ccagagcctc acttacgact g
//
LOCUS       DAJTRC010000232         2122 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.242, whole genome shotgun sequence.
ACCESSION   DAJTRC010000232 DAJTRC010000000
VERSION     DAJTRC010000232.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2122)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.242"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..122
                     /locus_tag="OO357_004135"
     CDS             <1..122
                     /locus_tag="OO357_004135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5888850.1"
                     /translation="PLKCELRGGQMRFTGLKRGYRLAEQVMMHEPLARIRWCG"
     gene            309..546
                     /locus_tag="OO357_004136"
                     /pseudo
     CDS             309..546
                     /locus_tag="OO357_004136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000544830.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            967..1545
                     /locus_tag="OO357_004137"
     CDS             967..1545
                     /locus_tag="OO357_004137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888851.1"
                     /translation="MKVSFKSLGYIFHDIYNKKHTIDEFNDVVRKAVLSGKINELNAC
                     HKVAIFLAEKDNEITKKDKAKIIDTLTENYSIEFQQLMNISERTLNSSLYITPGESGF
                     VSFVNREGKICHTAYVKSSDNSMTYYHANGSSIDKYITDMCGLICMRHIESTGIIFYM
                     LDEKVLSAIAEFMNEKGWRAAFCSAKNLYKCV"
     gene            complement(1654..1912)
                     /locus_tag="OO357_004138"
                     /pseudo
     CDS             complement(1654..1912)
                     /locus_tag="OO357_004138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611146.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            1896..2122
                     /locus_tag="OO357_004139"
                     /pseudo
     CDS             1896..2122
                     /locus_tag="OO357_004139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          668 a          377 c          518 g          559 t
ORIGIN      
        1 acccgctgaa gtgcgagctg cgcggaggtc agatgcggtt tacggggctg aagaggggtt
       61 accgtctggc agagcaggtt atgatgcatg agccgctggc tcgaatacgg tggtgcggct
      121 gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa cggagcaaaa aaccatcaga
      181 gatacattga atcaatcaat gtaagcaata ttaagtgtct ataccgatta cttgatgatc
      241 aggttgtgtt gaataaacgc gcattcttcg gtgctttttt aatcttttgg ggatttaaat
      301 tcctatcgat gaactgtcga tatgaaaaag cgagcatcat actgacgtca aacaaagggg
      361 tcgcagactg gggagaaatg ttcggagatc acgtgctggc aacagcgatc ctagatcggc
      421 tgctacacgc tgaatatcaa aggagagagt taccggttaa aagagaaacg taaagctgga
      481 gtgctgacca aaaacacaac gccaatcagt gatgatgaaa tggtggaaaa cggacagcat
      541 cagtaacgaa agtatcttag caggcatgaa aatcgtccgc atcccctaaa cggtcaaaat
      601 gcttctgccg tgaaggaagg agaatgccat ctcatcactt atatctggcg aatgaaacac
      661 agatggtcgg ccttgccccc aaacacacgg cggtgcaaaa tggaatggca gaaaacttcg
      721 tcaaaacgat gaaatatgat taaatcagtg ttatgttcga acctgacgtg ttaacagcag
      781 taaagtagtc tcgcgaaggt gttcaaacat tataatgaat ggcatcctca tagtgcgcag
      841 ggttatcgct tgctgcggaa atatctgaag cgacgtacca gtaatgggga aaatgatagc
      901 tggtttctga aaatatagag gaaaatgtga tatatatatc ctgttaatat ttaaggagaa
      961 tataaaatga aagtctcgtt taagtcttta ggctatattt ttcatgatat atataataaa
     1021 aaacacacta tcgatgagtt caacgatgtg gtaagaaaag cagttttatc aggtaaaatt
     1081 aacgagctga atgcatgtca taaagtggca atttttcttg ccgaaaaaga caatgagata
     1141 acaaagaaag acaaagccaa gataatagat acattaactg aaaactattc cattgaattt
     1201 caacaattaa tgaatattag tgagcgcacg ttaaattcat ctttatatat tactcctggt
     1261 gaaagtggtt ttgtcagttt tgttaatcgt gaaggaaaaa tatgtcatac tgcatatgta
     1321 aaatcatcag ataatagcat gacatattac catgcaaatg gtagcagcat tgacaaatat
     1381 attacggata tgtgcggcct tatttgtatg aggcatattg agtctactgg cattattttt
     1441 tatatgcttg atgaaaaagt tttatctgcc attgctgaat ttatgaatga aaaaggatgg
     1501 cgggctgcgt tttgttcagc aaagaactta tataaatgtg tttaatttta gaaaaactat
     1561 ttggtaaaat ccaacaagag tcgagaggtt cgcgttgctt cagcagggcc tcagcctgtt
     1621 caaccagact cagttgcagg tcgtgacgca gatttagtgg tggtggttga ttgtcagata
     1681 ccagccgatg atcttgtagt gcatatcatc agactttgag aagcatatgg ttttgcgggt
     1741 aagccgctta agatgtgtcc gcaggttcag attatgtcgc tctatccgct gagtgcattt
     1801 cttgcttata acatgactgg ttgaggacaa taaagttcgg tataccggct caggcatcgg
     1861 tcatgtagaa ggcaatgcta aactggctca gcaaactaag agcagcgcgc atccacttac
     1921 ccaatgcggt tgagaaaata gccttcgatc gctttcatgt ggcgaaacaa ccgggtgagg
     1981 tggtggataa aacccgccag aatgaacatc cgcatcttcc tgttgaaagt cgtcgtcagg
     2041 caaagggaac tcgcttcctg tggcagcaca gcgataaatg gatgaccgaa tccaggcagg
     2101 aaaagctgat atggctgcgt gc
//
LOCUS       DAJTRC010000233         2110 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.243, whole genome shotgun sequence.
ACCESSION   DAJTRC010000233 DAJTRC010000000
VERSION     DAJTRC010000233.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2110)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2110)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2110
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.243"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            376..519
                     /locus_tag="OO357_004140"
                     /pseudo
     CDS             376..519
                     /locus_tag="OO357_004140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
     gene            666..932
                     /gene="ospE1"
                     /locus_tag="OO357_004141"
     CDS             666..932
                     /gene="ospE1"
                     /locus_tag="OO357_004141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_001449237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspE1"
                     /protein_id="HCR5888852.1"
                     /translation="MLTQTIFPCLPQKQENIILEVSNPVLLSSTVTTDGYTVFNKKAA
                     IYELQIPAASRTKTLKFTATEMQWLTKINEAGIDEKQSQRYSDF"
     gene            1322..>2110
                     /gene="ipaH1.4"
                     /locus_tag="OO357_004142"
     CDS             1322..>2110
                     /gene="ipaH1.4"
                     /locus_tag="OO357_004142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH1.4"
                     /protein_id="HCR5888853.1"
                     /translation="MIKSTNIQAIGSGIMHQINNVYSLTPLSLPMELTPSCNEFYLKT
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQEAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTHLPDLPPMLKVIHAQFNQLESLPALPETLEELNAGDNKIKELPFLPENLTHLRVHN
                     NRLHILPLLPPELKLLVVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLRLAQNAFVNVAGNPLSGHTMRTLQQITTGPDY"
BASE COUNT          703 a          447 c          359 g          601 t
ORIGIN      
        1 gcttcttacc atatttgaaa taaagctcaa tagcgcgaat tttttgttct tcagtaaaca
       61 tgtttcatct tctgagagtc caggaaatcg tccgcatccc cattaccgtc ttatatcact
      121 ggcgctgaca tgtgctcatt gatgaagtga atctgaatga catgttttgc catatcaacg
      181 ccaaccggtg tatatttcat ttgtggagtc tccagtctga ggacgcttta tgcatcccgt
      241 attgggcagt atgatgccgg aaagctgcga ggctccacac ctgccagttt caccctttta
      301 accatcatac tactccgtgt ttaattgtgt gggatgcgtt cattacattt taacagtgtc
      361 accccgtcgg ccacgaaaga taaacaggtg accagaaaag ggatcttcat ccagcacatg
      421 ctatatcttg aacgtaacaa cctgaccctc cgcacccgca tcaagcggct ggcacgtaaa
      481 acaatctgct tctcgcgttc cgtggaaatc cacgaaaagt catcggctcc ttcattgaaa
      541 aacacatatt ctactgattg gaagcgccac ccgaaaaagt atcgtttttt tacagtaaac
      601 ttcatttagc cgactataat gtaaaaaaat aaatttttta ccaaaatata acataaggaa
      661 gtaatatgct tacacaaact atattccctt gtcttccaca aaaacaagaa aatatcatcc
      721 ttgaagtatc aaatcctgta ttattatctt ctactgttac tacagatgga tatactgttt
      781 tcaataaaaa agcagctata tacgaattac aaatacctgc tgccagccgg acgaaaacgt
      841 tgaaattcac tgctacagaa atgcaatggt tgacaaagat aaatgaagcc ggaattgatg
      901 aaaaacaaag ccaacggtat tctgatttct gaagttaatt tcagactcgt ttaagctcca
      961 ttttcaaaaa aaatatatca gggtttgtta ctaataactt ctgaaaacag aaaaaaatag
     1021 agcagtaatt ctatttcaga gaggagtaac tccatctatc ctcgatacgt actgaacgtt
     1081 atcgtgatat attcatcatg ctttcttcac cattattcga gtatagggag agacgcaatg
     1141 accttaaata cctttttatg gctgaattac cattctcagg aataacagca gactccttcc
     1201 catagacggc ttagggggaa agcaataccc ggagaaaggg tacaaagcaa actgttttaa
     1261 aattaataag ccatattcta tttaactaga attcttatcg ttgataagaa ttctagttat
     1321 aatgattaaa tcaaccaata tacaggcaat cggttctggc attatgcatc aaataaacaa
     1381 tgtatactcg ttaactccat tatctttacc tatggaactg actccatctt gtaatgaatt
     1441 ttatttaaaa acctggagcg aatgggaaaa gaacggtacc ccaggcgagc aacgcaatat
     1501 cgccttcaat aggctgaaaa tatgtttaca aaatcaagag gcagaattaa atttatctga
     1561 gttagattta aaaacattac cagatttacc gcctcagata acaacactgg aaataagaaa
     1621 aaacctatta acacatctcc ctgatttacc accaatgctt aaggtaatac atgctcaatt
     1681 taatcaactg gaaagcttac ctgccttacc cgagacgtta gaagagctta atgcgggtga
     1741 taacaagata aaagaattac catttcttcc tgaaaatcta actcatttac gggttcataa
     1801 taaccgattg catattctgc cactattgcc accggaacta aaattactgg tagtttctgg
     1861 aaacagatta gacagcattc ccccctttcc agataagctt gaagggctgg ctctggctaa
     1921 taattttata gaacaactac cggaattacc ttttagtatg aacagggctg tgctaatgaa
     1981 taataatctg acaacacttc cggaaagtgt cctgagatta gctcagaatg ccttcgtaaa
     2041 tgttgcaggt aatccattgt ctggccatac catgcgtaca ctacaacaaa taaccaccgg
     2101 accagattat
//
LOCUS       DAJTRC010000234         2104 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.244, whole genome shotgun sequence.
ACCESSION   DAJTRC010000234 DAJTRC010000000
VERSION     DAJTRC010000234.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2104)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.244"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..154
                     /locus_tag="OO357_004143"
     CDS             <1..154
                     /locus_tag="OO357_004143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708937.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates concomitant with their translocation across the
                     cell membrane;protein IIA transfers a phosphoryl group to
                     IIB which then transfers the phosphoryl group to the
                     sugar; IIC forms the translocation channel for the sugar
                     uptake; involved in N-acetylgalactosamine transport;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCR5888854.1"
                     /translation="CYIRVSNLLPVAVLGAGFAVYEFFNAKSRQQAQPQPVASKNEEE
                     DYSNGI"
     gene            144..935
                     /gene="agaD"
                     /locus_tag="OO357_004144"
     CDS             144..935
                     /gene="agaD"
                     /locus_tag="OO357_004144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IID"
                     /protein_id="HCR5888855.1"
                     /translation="MGSEISKKDITRLGFRSSLLQASFNYERMQAGGFTWAMLPILKK
                     IYKDDKPGLSAAMKDNLEFINTHPNLVGFLMGLLISMEEKGENRDTIKGLKVALFGPI
                     AGIGDAIFWFTLLPIMAGICSSFASQGNLLGPILFFAVYLLIFFLRVGWTHVGYSVGV
                     KAIDKVRENSQMIARSATILGITVIGGLIASYVHINVVTSFAIDSTHSVALQQDFFDK
                     VFPNILPMAYTLLMYYFLRVKKAHPVLLIGVTFVLSIVCSAFGIL"
     gene            936..1691
                     /locus_tag="OO357_004145"
     CDS             936..1691
                     /locus_tag="OO357_004145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactosamine-6-phosphate isomerase"
                     /protein_id="HCR5888856.1"
                     /translation="MERGTASGGASLLKEFHPVQTLQQVENYTALSERASEYLLAVIR
                     SKPDAVICLATGATPLLTYHYLVEKIHQQQVDISQLTFVKLDEWVDLPLTMPGTCETF
                     LQQHIVQPLGLREDQLISFRSEEINETECERVTNLIARKGGLDLCVLGLGKNGHLGLN
                     EPGESLQPACHISQLDARTQQHEMLKTTGRPVTRGITLGLKDILNAREVLLLVTGEGK
                     QDATERFLTAKVSTAIPASFLWLHSNFICLINT"
BASE COUNT          578 a          467 c          486 g          573 t
ORIGIN      
        1 ttgctacatc cgggtcagca acctgttgcc ggttgccgta ctgggcgcag gctttgcggt
       61 gtatgagttt ttcaatgcga aatcccggca gcaagcgcaa ccgcagcccg ttgccagtaa
      121 aaatgaagaa gaggactaca gcaatgggat ctgaaatcag taaaaaagat atcacccgtc
      181 tgggctttcg ttcgtcgctg ctgcaagcga gctttaacta cgaaaggatg caggcgggcg
      241 gttttacctg ggcgatgttg ccgatcctga aaaagattta taaggacgac aaaccgggct
      301 taagcgcggc gatgaaagat aacctcgaat ttattaatac ccacccgaat ctggtcggat
      361 tcctgatggg gttattaatt tcgatggaag aaaaaggaga aaaccgcgac accattaaag
      421 gcctcaaagt ggcactgttt ggcccaatcg ccgggattgg cgatgcgatt ttctggttta
      481 ctttgttgcc gattatggcg ggaatttgct catcatttgc cagccaggga aacctgctgg
      541 ggccgattct atttttcgcc gtttacctgc ttatcttttt cctgcgcgtc ggctggaccc
      601 acgtcggtta ttcagtcggc gtgaaggcga tcgataaagt gcgagagaac tcgcagatga
      661 ttgctcgctc ggcaaccatc ctcgggatca cggtaatcgg cgggctgatc gcttcgtatg
      721 tgcatattaa cgtggtgaca tcgtttgcca tcgacagtac ccacagcgtc gcgctgcaac
      781 aggatttctt cgataaagtc ttcccgaaca ttttaccgat ggcctacacc ctgctgatgt
      841 attacttcct gcgggtgaaa aaagcgcatc cggtgctgtt aatcggcgtg acttttgtgc
      901 tctctattgt ttgttccgca ttcggcattt tgtaaatgga acgaggcact gcttctggcg
      961 gtgcctcttt acttaaggaa tttcatcctg tgcaaaccct tcagcaagtt gaaaactata
     1021 cggcgttaag tgaacgagcc agcgaatatt tattggctgt gatccgtagc aaaccggatg
     1081 ccgtgatttg cctggcgacc ggagccacgc cattactgac gtatcattat ctggtagaaa
     1141 aaatccacca gcagcaggtt gatatcagcc agctcacctt cgtgaagctc gacgaatggg
     1201 tggatctgcc attaacgatg cctggcacct gcgaaacttt cctacaacag catatcgtgc
     1261 agccgctggg gctacgtgaa gaccagctca tcagctttcg ctccgaagag ataaatgaga
     1321 cagagtgcga acgggtaacg aacctgattg cgcgcaaagg cggtctggat ttatgcgttc
     1381 tcggattggg gaaaaacggt catcttgggc tgaacgaacc gggagaaagc ctacaaccgg
     1441 cctgccatat cagtcaactt gatgccagaa cacagcaaca tgagatgtta aaaaccacgg
     1501 gtcgccccgt gactcgtggg atcaccttag gcctgaagga tattctcaat gcccgcgagg
     1561 ttttgttatt ggtgactggc gaaggaaagc aggatgcgac agaacgtttt ctcacggcta
     1621 aagtctccac cgctatcccg gcttcatttt tatggctgca cagtaacttt atttgtttaa
     1681 ttaataccta accagatgaa aaatttgctc aaaattttta aattaactta ttcaacagtc
     1741 acgcattata ttaaataaca tttgactggg ttgaacataa cgccaatagc aaaaggagtt
     1801 atgttcaaca atttaatttt agatatacat ataacttgtt ctattaataa atcacctcgt
     1861 attctgactc ggcccctggc gcagcaaagt caggcattta tactcttttt aacacataga
     1921 aaattcatat caatataata aaaacataaa atatttgcat atacatataa tcttaaagtt
     1981 tagtctattt aatgttcaat gaaatatttc tacctgtata atctttaaag atgttgaaca
     2041 tatattcaca ttaaatatga ttatgtactt gttacaagga taaggttata aatgaataaa
     2101 gtta
//
LOCUS       DAJTRC010000235         2095 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.245, whole genome shotgun sequence.
ACCESSION   DAJTRC010000235 DAJTRC010000000
VERSION     DAJTRC010000235.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2095)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2095)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2095
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.245"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            39..1436
                     /gene="ccp"
                     /locus_tag="OO357_004146"
     CDS             39..1436
                     /gene="ccp"
                     /locus_tag="OO357_004146"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HCR5888857.1"
                     /translation="MKMVSRITAIGLAGVAICYLGLSGYVWYHDNKRSKQADVQASAV
                     SENNKVLGFLREKGCDYCHTPSAELPAYYYIPGAKQLMDYDIKLGYKSFNLEAVRAAL
                     LADKPVSQSDLNKIEWVMQYETMPPTRYTALHWAGKVSDEERAEILAWIAKQRAEYYA
                     SNDTAPEHRNEPVQPIPQKLPTDAQKVALGFALYHDPRLSADSTISCAHCHTLNAGGV
                     DGRKTSIGVGGAVGPINAPTVFNSVFNVEQFWDGRAATLQDQAGGPPLNPIEMASKSW
                     DEIIAKLEKDPQLKAQFLEVYPQGFSGENITDAIAEFEKTLITPDSPFDKWLRGDENA
                     LTAQQKKGYQLFKDNKCATCHGGIILGGRSFEPLGLKKDFNFGEITAADIGRMNVTKE
                     ERDKLRQKVPGLRNVALTAPYFHRGDVPTLDGAVKLMLRYQVGKELPQEDVDDIVAFL
                     HSLNGVYTPYMQDKQ"
     gene            1647..>2095
                     /locus_tag="OO357_004147"
     CDS             1647..>2095
                     /locus_tag="OO357_004147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate decarboxylase"
                     /protein_id="HCR5888858.1"
                     /translation="MDQKLLTDFRSELLDSRFGAKAISTIAESKRFPLHEMRDDVAFQ
                     IINDELYLDGNARQNLATFCQTWDDENVHKLMDLSINKNWIDKEEYPQSAAIDLRCVN
                     MVADLWHAPAPKNGQAVGTNTIGSSEACMLGGMAMKWRWRKRMEAAG"
BASE COUNT          568 a          438 c          542 g          547 t
ORIGIN      
        1 tctgggtcac aataactcac caccgtgagg gaagaataat gaaaatggtc tcacgtatta
       61 ccgcgatcgg cctggctggc gtcgcgattt gctatttagg gttatctggt tatgtgtggt
      121 accacgataa taaacgcagt aaacaggccg atgttcaggc atctgctgtc agtgaaaata
      181 ataaggtttt aggctttctc cgcgaaaaag gatgcgacta ttgccacacg ccttcggcag
      241 aattacccgc ctattattat attcctggcg cgaaacagtt gatggattac gacattaagc
      301 ttggatataa atcttttaac ctcgaggccg tgcgtgcggc actgctggct gataaacccg
      361 tttcgcaaag cgatctgaat aagattgaat gggtgatgca gtatgaaact atgccaccaa
      421 cgcgttatac cgcgctacac tgggcgggta aggtgagtga tgaagagcgg gcggaaatac
      481 tggcctggat tgcaaaacag cgcgcggaat attacgccag caatgatact gctccggagc
      541 atcgcaatga accggtgcag cccatcccgc aaaaactgcc taccgatgcg caaaaagtgg
      601 cgttgggctt tgcgctgtat cacgatcccc gtttatcggc tgatagcacc atttcatgcg
      661 ctcattgcca tacgttgaat gcggggggcg tcgatggcag aaaaacatcg attggtgttg
      721 gtggcgcagt tgggccgatt aatgcgccga cggtatttaa ctcagtattt aacgttgagc
      781 agttctggga tggtcgtgcg gcaacattgc aggatcaggc aggtggaccg ccgttgaacc
      841 cgattgaaat ggcgtcgaaa tcctgggacg aaattattgc taagctggaa aaagatccgc
      901 agcttaaagc gcagttcctc gaagtttatc cgcaaggttt cagtggcgaa aatattactg
      961 atgccattgc tgaatttgag aaaacattaa ttacgccgga ttccccattt gataaatggt
     1021 tgcgtggaga tgaaaatgcg ctgacggcgc aacagaaaaa aggctatcaa ttatttaaag
     1081 ataataaatg tgcaacttgt catggtggta ttattctcgg cggacgttcc tttgaaccgt
     1141 tggggctgaa aaaagacttt aactttgggg aaattacggc ggcggatatt ggtcgtatga
     1201 atgtgactaa agaagagcgt gataaattgc gtcagaaagt acccggttta cgtaacgttg
     1261 ctttaacggc accgtacttc catcgcggtg acgtgccgac gctggacggg gcggtgaaac
     1321 tgatgctgcg ctatcaggta ggcaaagagc tgccgcagga ggatgtggat gatatcgtag
     1381 ctttcctgca cagtctgaac ggggtgtaca cgccgtatat gcaggataaa caataattaa
     1441 tttgatcgcc cgaacaggaa tgtttgggcg atttttatta cgataataaa gtctgttttt
     1501 aatattatca tgttaaatgt ttatattata aaaagtcgtt tttctgctta ggattttgtt
     1561 atttaaatta agcctgtaat gccttgcttc cattgcggat aaatcctact tttttattgc
     1621 cttcaaataa atttaaggag ttcgaaatgg accagaagct gttaacggat ttccgctcag
     1681 aactactcga ttcacgtttt ggcgcaaagg ccatttctac tatcgcggag tcaaaacgat
     1741 ttccgctgca cgaaatgcgc gatgatgtcg cattccagat tatcaatgat gaattatatc
     1801 ttgatggcaa cgctcgtcag aacctagcca ctttctgcca gacctgggac gacgaaaacg
     1861 tccataaatt gatggatttg tcgatcaata aaaactggat cgacaaagaa gaatatccgc
     1921 aatccgcagc catcgacctg cgttgcgtaa atatggttgc cgatctgtgg catgcgcctg
     1981 cgccgaaaaa tggtcaggcc gttggcacca acaccattgg ttcttccgag gcctgtatgc
     2041 tcggcgggat ggcgatgaaa tggcgttggc gcaagcgtat ggaagctgca ggcaa
//
LOCUS       DAJTRC010000236         2091 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.246, whole genome shotgun sequence.
ACCESSION   DAJTRC010000236 DAJTRC010000000
VERSION     DAJTRC010000236.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.246"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            1..798
                     /locus_tag="OO357_004148"
                     /pseudo
     CDS             1..798
                     /locus_tag="OO357_004148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001608690.1"
                     /note="internal stop; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            913..1254
                     /locus_tag="OO357_004149"
                     /pseudo
     CDS             913..1254
                     /locus_tag="OO357_004149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000952428.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
     gene            1237..2074
                     /locus_tag="OO357_004150"
                     /pseudo
     CDS             1237..2074
                     /locus_tag="OO357_004150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000244358.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
BASE COUNT          516 a          506 c          632 g          437 t
ORIGIN      
        1 ctgggccttg cccgtagcag ttatttttat caccgggctg cactgaaagc cggtgataaa
       61 tacgccacga tacgtaagat gctgacagat atctttaaca gtaattacca gtgttatggc
      121 tatcgtcgcc tgcatgcgat gctcaggcat gagggggggc ggctatcaga aaaggttgta
      181 cgcagactta tggtggagga acagcttgtc gtcagtcgta accgtcgtcg tcgctacagc
      241 tcatattgcg gagaaatcgg accggctccg gataacctta tcgccagaga ttttaaggcg
      301 gagcaaccta atcagaaata gctgacagat atcacggagt tccagctccc tgcaggtaaa
      361 gtctggctgt caccagtggt ggactgcttc gatggaaaag ttgtgagctg gtctctcagt
      421 acacgccctg atgctgaact ggtcaacact atgctggata gcgctgtcga aacgttaaat
      481 gctggcgaac gaccggtgat acacagtgac agaggtgggc attatcgctg gccaggctgg
      541 ctggaaagag tgaatgcagc aggtcttatt cgctcaatgt cccgtaaagg atgttcacct
      601 gataatgctg catgcgaagg ctttttcggc agactgaaaa cggaaatgta ttatgggcgt
      661 aaatggtcgg gcatcacgcc agaaaagttc atgcagcaag tggatgctta catcagatgg
      721 tataacgagc ggcgtataaa attatcgctg ggtgccgtca gccccaaaat gtaccgccaa
      781 caatgcgggc tggaatgata aagcagtcca ggaaatcgtc cgcatccccc cttcaccatt
      841 tggattgtcc gctccaccca acatgttgtt tccttaaggt tcctacacca caaaggaaac
      901 tctcatgctg cgcagtggat ggctgacgtt gccgacaggc gggaacttcg ccagttcagg
      961 cagacaccgg aacagcgctt cacgcaggaa caggagcatc tgcagccgct gctgggtacg
     1021 gacttcgata tccgtcatgt ctcctgggat ggctatatcg aggttggcgg gaaccgttac
     1081 agtgtgccgg aaagcctgtg tggtcagctg gtatcaatac ggatctcgct ggatgatgaa
     1141 ctgcggatct acagtaatga gcaacaggta gcatcgcacc gtctctgttc ggctgcatac
     1201 ggctggcaga ccgtgccgga gcatcacgct ccactctggc agcaggcttc ggagcgcatg
     1261 gccgaatggc tgggggagat acagaaacgg gtaatcactg tctgtgaccg ggaagcggat
     1321 atctggcatt acctgtatta taaagtcagc cacggacagc gtggtgcgtg ctgcacagaa
     1381 tcgccggctg gaagaggcac ccggcaagct cttcgaactg ccggaagtcc tggcaaccgc
     1441 aggaagccac acgctgaatg tgatgcaaaa aggcgggcgg gcagcccgtc aggcccggat
     1501 gttcatcagt tacagcgaag tcagcataaa aaatcccgac aacagcggcc aggcgctccc
     1561 gctcacgtat gtctgttgcc gggagcaggc agaggacggt gcctgctggc atctgctgac
     1621 gtcaggaaaa gcggcgagtg ccgccgatgc acgacgtatt gtcagccatt acgagcgacg
     1681 ctggctgaca gaggaatacc acaaggcgtg gaaaagtggt ggtacatgga atcgctgcga
     1741 atgcagaccc gggataacct tgagcgcatg gtggttatcc tggcgtttat agcggtacgg
     1801 gtgctgggcc tgcggcaggg aggtgtcagt gaagaaacac aaaatgacag ctgtgagaaa
     1861 atactgacgc cgacagagtg gaaactactg tgggttaagc tggaaggaaa gccgcttccg
     1921 gtgcaggcac cgaccctgaa atgggcctgg ggcgatggca tgacagcaaa cgcacaggtc
     1981 gtcccggttg gggcgtcatg tgggatggct ggtcagactt caggatatgg ttgagggtta
     2041 cctggtgatg aagtctcttg atcaggagct ctgatcaaga gacagcagct t
//
LOCUS       DAJTRC010000237         2047 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.247, whole genome shotgun sequence.
ACCESSION   DAJTRC010000237 DAJTRC010000000
VERSION     DAJTRC010000237.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.247"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            6..224
                     /locus_tag="OO357_004151"
     CDS             6..224
                     /locus_tag="OO357_004151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4014 family protein"
                     /protein_id="HCR5888859.1"
                     /translation="MVTLFRKKYPRKSRTTEFLFLILFIVLMTPISPQIFVWIIGKII
                     EPVIELYNDVVWASFNTLHNKINPYKES"
     gene            226..591
                     /locus_tag="OO357_004152"
     CDS             226..591
                     /locus_tag="OO357_004152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HCR5888860.1"
                     /translation="MALTKKQRAELRMKFGGRCAYCGCELGEKWHADHVKPVIRFDGN
                     MLHQERDDISNMVPECHPCNLHKHCSSLEDYRRIISDGRREFLASGKGKALVRMGLVE
                     MKSDPVVFWFEIYQEGATA"
     gene            588..1253
                     /locus_tag="OO357_004153"
     CDS             588..1253
                     /locus_tag="OO357_004153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005115317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888861.1"
                     /translation="MTTITKERLQWLANISGRDDIDDIDGGEIRELALIALASLEAEP
                     VAECIVEDGGMCIDGFGEYVGHSLPDGMHELYAAPRMRQPDGWKAVAVAWKVTFTQVD
                     QESNTFTAIYFYKAEVERWVRLHKACDFRADITPLYAAPAVPVAVNDDMAYAFHHALS
                     DSSLGSDEIEEIKTGLRAAFANVTIQPEPIVPDEIGPNDSNTFDYVDGWNACRAAMLK
                     GDK"
     gene            1253..1618
                     /locus_tag="OO357_004154"
     CDS             1253..1618
                     /locus_tag="OO357_004154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000610380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HCR5888862.1"
                     /translation="MINRTKLEHILEYARQQKCIGQLCKIPPGDMVEIVEMAMRKAGN
                     SPVTLAGWISCSDAVPAEYCDVILLDDLGNVFPGSWDKVFCPTRGGNKMAFVDKDGVE
                     VESSTHWMPLPEPPQEVNQ"
BASE COUNT          558 a          423 c          543 g          523 t
ORIGIN      
        1 aatgaatggt tacattattt agaaaaaaat atccgcgaaa gagtagaaca acagaattcc
       61 tgtttctcat tctgtttatc gtgttgatga caccgatatc cccgcaaatt tttgtctgga
      121 taatcggaaa aataattgag ccagttattg aattgtataa cgacgtggta tgggcgtcat
      181 tcaacacact gcacaataaa attaatccgt ataaggaaag ctgatatggc actgacgaaa
      241 aaacaacgtg cagaactgcg catgaagttc ggtggtcgct gcgcttattg cggctgcgaa
      301 cttggcgaaa agtggcatgc agaccatgta aaaccggtca ttcgttttga tggaaatatg
      361 cttcaccagg aacgtgacga tatatccaac atggtaccag aatgccaccc atgcaatctg
      421 cacaagcatt gcagtagcct ggaagattat cgacgaatta tcagtgatgg tcgtcgtgaa
      481 ttccttgcgt ccgggaaagg caaagcgctg gttcgtatgg gattggttga aatgaaatct
      541 gacccggttg tgttctggtt tgaaatatat caagaagggg ctacggcatg actactatta
      601 ccaaagagcg actgcaatgg ctggctaaca tttctggccg cgatgatatt gacgatatag
      661 acggcggtga aattcgtgaa ctggcgctta tcgctctggc atcactggaa gcagagcctg
      721 tagcggaatg cattgttgaa gatgggggca tgtgtattga tgggttcggt gagtatgtgg
      781 gtcactcgct gcctgatgga atgcatgagc tttatgctgc cccgcgaatg cgacaacctg
      841 atggatggaa agccgtagct gtagcgtgga aggtgacgtt tactcaagtt gaccaggaat
      901 ctaatacgtt cactgctata tatttttaca aagcggaagt tgagcgttgg gtacgactgc
      961 ataaagcatg tgattttcgg gcagatataa caccgcttta cgcagcgccg gcagtgccgg
     1021 ttgcagtaaa cgacgacatg gcttacgcat tccatcacgc actgtcagat tcatcgcttg
     1081 gctctgatga aatcgaagaa attaaaaccg gtttgcgtgc tgcctttgcc aacgtcacta
     1141 tccaaccaga gccgatagtg ccggatgaaa tcgggccaaa cgatagtaat acgtttgatt
     1201 atgttgatgg ctggaacgcc tgccgcgctg ccatgcttaa gggagataaa taatgattaa
     1261 tcgaaccaaa ctggagcaca ttctcgaata tgccaggcag cagaaatgta ttgggcaact
     1321 ttgtaaaatt ccaccaggag atatggttga aatcgtggaa atggccatgc gtaaggctgg
     1381 caactctccg gtaactctgg ctggctggat aagctgtagt gatgcagttc ctgctgaata
     1441 ttgcgatgtg attcttctcg atgatctcgg gaatgtattc cccggttcct gggataaggt
     1501 tttttgcccc actcgtggcg ggaataagat ggcttttgtg gacaaagacg gcgttgaagt
     1561 agagagctca actcactgga tgccgctacc ggaaccaccg caggaggtga atcaatgacc
     1621 tggcctgagg cattcaccac ggtaggaatt gcgatggcgg tggcgctggt ggtgtattcg
     1681 atttgccgtt ggggttaaca aacaaaaacc ccggattgat ggtccggggt ttttgaagga
     1741 aacaaaacag aaacagcaat tgccgttacc tgttgttacc atggcaagta aacgtatctc
     1801 aggcgagcgc attgcgccgt tctgacgcag ataaattagc ctggatagaa ggtgctggca
     1861 ataaaaaata gcgttttctt atcggtatcg gtaagattgc tgcgggtgct tgaggctgtc
     1921 tgcctcgggc atgccactgt aaggcagaca gagaaaagcc ccagttaaca ttacgcgtcc
     1981 tgcaagacgc ttaacattaa tctgaggccc aatctatgtc tcacaaatgt aggttagcct
     2041 cttacgt
//
LOCUS       DAJTRC010000238         2042 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.248, whole genome shotgun sequence.
ACCESSION   DAJTRC010000238 DAJTRC010000000
VERSION     DAJTRC010000238.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.248"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..348)
                     /gene="arsC"
                     /locus_tag="OO357_004155"
     CDS             complement(<1..348)
                     /gene="arsC"
                     /locus_tag="OO357_004155"
                     /EC_number="1.20.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-dependent arsenate reductase"
                     /protein_id="HCR5888863.1"
                     /translation="MSNITIYHNPACGTSRNTLEMIRNSGTEPTIIHYLETPPTRDEL
                     VKLIADMGISVRALLRKNVEPYEELGLAEDKFTDDRLIDFMLQHPILINRPIVVTPLG
                     TRLCRPSEVVLEIL"
     gene            complement(361..1650)
                     /gene="arsB"
                     /locus_tag="OO357_004156"
     CDS             complement(361..1650)
                     /gene="arsB"
                     /locus_tag="OO357_004156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite/antimonite:H(+) antiporter ArsB"
                     /protein_id="HCR5888864.1"
                     /translation="MLLAGAIFVLTIVLVIWQPKGLGIGWSATLGAVLALVTGVVHPG
                     DIPVVWNIVWNATAAFIAVIIISLLLDESGFFEWAALHVSRWGNGRGRLLFTWIVLLG
                     AAVAALFANDGAALILTPIVIAMLLALGFSKGTTLAFVMAAGFIADTASLPLIVSNLV
                     NIVSADFFGLGFREYASVMVPVDIAAIVATLVMLHLYFRKDIPQNYDMALLKSPAEAI
                     KDPATFKTGWVVLLLLLVGFFVLEPLGIPVSAIAAVGALILFVVAKRGHAINTGKVLR
                     GAPWQIVIFSLGMYLVVYGLRNAGLTEYLSGVLNVLADNGLWAATLGTGFLTAFLSSI
                     MNNMPTVLVGALSIDGSTASGVIKEAMVYANVIGCDLGPKITPIGSLATLLWLHVLSQ
                     KNMTISWGYYFRTGIIMTLPVLFVTLAALALRLSFTL"
     gene            complement(1704..>2042)
                     /gene="arsR"
                     /locus_tag="OO357_004157"
     CDS             complement(1704..>2042)
                     /gene="arsR"
                     /locus_tag="OO357_004157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="As(III)-sensing metalloregulatory
                     transcriptional repressor ArsR"
                     /protein_id="HCR5888865.1"
                     /translation="PIQLFKILADETRLGIVLLLSELGELCVCDLCTALDQSQPKISR
                     HLALLRESGLLLDRKQGKWVHYRLSPHIPAWAAKIIDEAWRCEQEKIQAIVRNLARQN
                     CSGDSKNICS"
BASE COUNT          551 a          562 c          517 g          412 t
ORIGIN      
        1 cagaatttcc agcaccactt ctgaagggcg gcacaggcga gttcccagcg gcgtcaccac
       61 aatcgggcga ttaatcagaa tcgggtgctg aagcataaag tcgattaacc gatcgtcagt
      121 aaatttatct tccgcaaggc ccagctcctc atacggttcg acgtttttac gcagcagcgc
      181 gcgtacggaa atccccatat cggcaatgag tttgaccagt tcatcgcgcg ttggcggagt
      241 ttccagataa tggataatgg tcggttctgt gccgctgttg cggatcatct ccagcgtatt
      301 acgcgacgtg ccgcaggccg ggttgtgata aatggtaatg ttgctcatat cagtatctca
      361 ttacaaagtg aaagagagac gtagcgccag cgcagccagc gtcacaaaca gcacaggcag
      421 ggtcatgata atccctgtac ggaagtaata tccccagctg atagtcatat tcttctgcga
      481 aagtacgtgc agccagagca gcgttgccag gctaccaatc ggggtaattt tcggccccaa
      541 atcgcagcca atcacgttgg cataaaccat cgcttctttg ataacgccag atgccgtgct
      601 gccatcaatg gacaacgcgc caaccagtac cgtcggcata ttgttcataa tagaagagag
      661 gaaggcggtg aggaatccgg tgccgagcgt cgcggcccac aggccgttat ccgccagcac
      721 gttgagtacg ccagaaagat attccgttaa tccggcattg cgcaggccat aaaccaccag
      781 atacatgccg agcgagaaga tgacaatctg ccagggggca ccgcgcagga ctttacccgt
      841 attaatcgca tgaccgcgtt tagcgacgac aaataatatc agtgcgccca cagctgcaat
      901 ggcgctcacc ggaatgccga gcggttccag gacgaaaaat cccaccagca gaagcagtaa
      961 aacaacccag ccagttttga acgtagcagg atctttgatc gcttctgcgg gagatttcag
     1021 cagcgccata tcgtagttct gcggaatatc tttgcgaaaa tagagatgta gcatcaccag
     1081 cgtggcaaca atcgcggcga tatccaccgg caccatcacc gaggcgtatt cgcgaaagcc
     1141 gaggccaaag aaatcagcgg aaacgatatt caccaggttg gagacaataa gcggcaggct
     1201 ggcggtatcg gcaatgaatc cggccgccat cacgaacgcc agcgtcgtgc ctttactgaa
     1261 tcctaaagcc agcagcatgg cgatgacaat cggcgtcaaa ataagcgccg cgccatcatt
     1321 ggcaaacagg gccgcaacgg cggcaccgag caggacaatc caggtaaaca gcaagcgacc
     1381 gcgtccattg ccccagcgtg aaacgtgcag cgctgcccat tcaaaaaagc cggactcatc
     1441 cagcagcagg ctgatgataa tgacggcaat aaacgccgcc gtcgcgttcc agacgatatt
     1501 ccacaccacc ggaatatcac ccggatgaac cacgcccgta accaacgcca gtaccgcgcc
     1561 gagcgttgca ctccagccga tgcctaaacc tttcggctgc cagataacca atacgatggt
     1621 caggacaaag atagcgcctg ccagtaacat aatgcctccc ggataaaaca catgtgcaaa
     1681 tccatatgtg tttagctaaa tttttaactg caaatgttct tactgtctcc ggaacagttt
     1741 tgtctggcca ggttgcggac aattgcctga atcttttcct gttcacatcg ccaggcttca
     1801 tcaataattt tcgccgccca tgctggaata tgcggcgata agcggtaatg aacccactta
     1861 ccttgcttgc ggtccagcaa tagcccgctt tcacgcagca atgccagatg gcgggatatc
     1921 ttgggctgcg actgatcgag ggcagtgcag agatcgcaga cgcataactc tcccagttcg
     1981 ctaagcagta aaacgatgcc cagacgggtt tcatcagcaa gaattttgaa caattggatg
     2041 gg
//
LOCUS       DAJTRC010000239         2033 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.249, whole genome shotgun sequence.
ACCESSION   DAJTRC010000239 DAJTRC010000000
VERSION     DAJTRC010000239.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.249"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            75..650
                     /gene="ssuE"
                     /locus_tag="OO357_004158"
     CDS             75..650
                     /gene="ssuE"
                     /locus_tag="OO357_004158"
                     /EC_number="1.5.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706858.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HCR5888866.1"
                     /translation="MRVITLAGSPRFPSRSSSLLEYAREKLNGLDVEVYHWNLQNFAP
                     EDLLYARFDSPALKTFTEQLQQADGLIVATPVYKAAYSGALKTLLDLLPERALQGKVV
                     LPLATGGTVAHLLAVDYALKPVLSALKAQEILHGVFADDSQLIDYHHRPQFTPNLQTH
                     LDTALETFWQALHRRDVQVPDLLSLRGNAHA"
     gene            643..1602
                     /gene="ssuA"
                     /locus_tag="OO357_004159"
     CDS             643..1602
                     /gene="ssuA"
                     /locus_tag="OO357_004159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415456.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aliphatic sulfonate ABC transporter
                     substrate-binding protein SsuA"
                     /protein_id="HCR5888867.1"
                     /translation="MRNIIKLALAGLLSVSTFAVAAESSPEALRIGYQKGSIGMVLAK
                     SHQLLEKRYPQSKISWVEFPAGPQMLEALNVGSIDLGSTGDIPPIFAQAAGADLVYVG
                     VEPPKPKAEVILVAENSPIKTVADLKGHKVAFQKGSSSHNLLLRALRQAGLKFTDIQP
                     TYLTPADARAAFQQGNVDAWAIWDPYYSAALLQGGVRVLKDGTDLNQTGSFYLAARPY
                     AEKNGAFIQGVLATFSEADALTRSQREQSIALLAKTMGLPAPVIASYLDHRPPTTIKP
                     VNAEVAALQQQTADLFYENRLVPKKVDIRQRIWQPTQLEGKQL"
     gene            1599..>2033
                     /locus_tag="OO357_004160"
     CDS             1599..>2033
                     /locus_tag="OO357_004160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5888868.1"
                     /translation="MSLNMFWFLPTHGDGHYLGTEEGSRPVDHGYLQQIAQAADRLGY
                     TGVLIPTGRSCEDAWLVAASMIPVTQRLKFLVALRPSVTSPTVAARQAATLDRLSNGR
                     ALFNLVTGSDPQELAGDGVFLDHSERYEASAEFTQVWRRLLLG"
BASE COUNT          467 a          558 c          534 g          474 t
ORIGIN      
        1 tgtctctccg acagactgac cgaattgtta tcaatgttaa caaaaaaaag aacaattggt
       61 tataaggaga gagtatgcgt gtcatcaccc tggcgggtag tcctcgcttt ccttctcgct
      121 ccagctcctt gctggaatat gcgcgggaaa aactaaatgg cctagatgta gaggtttatc
      181 actggaatct gcaaaacttc gccccggaag atctgcttta tgctcgtttc gatagtccgg
      241 cactcaagac cttcaccgaa cagctgcaac aggctgatgg gctgattgtc gccacgcctg
      301 tgtataaagc cgcctattcc ggcgcgctga aaaccctgct cgacctgctg ccagagcgcg
      361 ctttgcaagg caaagtggtg ctaccgctgg cgacgggcgg taccgtggcc catctgctgg
      421 cggtcgatta tgcccttaaa ccagttttaa gcgcactgaa agctcaggag atcctgcacg
      481 gcgtgtttgc cgatgactca caattaattg attaccatca cagaccccag ttcacgccaa
      541 atctgcaaac ccatcttgat accgcgctag aaactttctg gcaggcattg caccgccgcg
      601 atgttcaggt tcctgacctt ctgtctctgc gaggtaatgc ccatgcgtaa catcattaaa
      661 ctggcgctgg cgggattgct tagcgtctct acttttgcgg ttgctgcaga atcctcgcct
      721 gaagcgttac gtataggcta tcagaaaggc agtattggta tggtgctggc aaaaagccac
      781 cagttactgg aaaaacgcta tccgcaatca aaaatctcct gggtggagtt ccccgcgggt
      841 ccgcaaatgt tggaagcgtt aaacgttggc agtattgatc tcggcagtac cggggatatt
      901 ccgccaatct ttgcccaggc tgccggggct gatttggtat atgtgggcgt cgagccaccg
      961 aagcccaaag ccgaagtgat tctggtggca gaaaacagcc cgatcaaaac cgtagccgat
     1021 cttaaaggtc acaaagttgc ctttcagaaa ggttccagtt cacacaacct tttactgcgt
     1081 gcactgcgtc aggccggact taaattcacc gatatccagc ccacttacct gacgcctgcc
     1141 gatgcccgcg ccgcgttcca gcaaggtaac gttgacgcct gggctatctg ggatccctac
     1201 tactccgctg cgttattaca gggcggcgtg cgggtgctga aagacggcac cgatctcaat
     1261 caaactggat cgttttatct ggcagctcgt ccctatgcag aaaaaaacgg cgcttttatt
     1321 cagggcgtac tggcaacctt tagtgaggcc gatgcgttaa cccgcagcca gcgcgaacaa
     1381 agcatcgctt tactggcaaa aacgatgggc ttaccggcac cggtgattgc ctcttactta
     1441 gatcatcgcc ctcctaccac catcaaaccg gttaacgccg aggttgccgc cttacagcag
     1501 caaacggcag atctgtttta tgaaaaccgt ctggtgccga aaaaagtcga tattcgccag
     1561 cgcatctggc agcccactca actggaagga aaacaattat gagtctgaat atgttctggt
     1621 ttttaccgac ccacggtgac gggcactatc tgggaacgga agaaggttca cgcccggttg
     1681 atcacggtta tctgcaacaa attgcgcaag cggcggatcg tctgggctat accggtgtgc
     1741 tgatcccaac ggggcgatcg tgtgaagatg cgtggctggt tgcagcgtcg atgatcccgg
     1801 tgacgcagcg gctgaagttt cttgtcgccc tgcgtcccag cgtaacctca cctactgttg
     1861 ccgcccgcca ggccgccacg cttgaccgtc tctcaaatgg tcgtgcgttg tttaatctgg
     1921 tcacaggcag cgatccacaa gagctggcag gcgacggcgt gttccttgat catagcgagc
     1981 gctacgaagc ctcggcggaa tttacccagg tctggcggcg cttattgctg ggg
//
LOCUS       DAJTRC010000240         2032 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.250, whole genome shotgun sequence.
ACCESSION   DAJTRC010000240 DAJTRC010000000
VERSION     DAJTRC010000240.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2032)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2032)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2032
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.250"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(210..374)
                     /locus_tag="OO357_004161"
     CDS             complement(210..374)
                     /locus_tag="OO357_004161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888869.1"
                     /translation="MTLTTTALNGSSSRRFEGCVWTPPLISHTALHPTSRHWMHSWHT
                     YAGNAEIPGL"
     gene            1276..1560
                     /locus_tag="OO357_004162"
     CDS             1276..1560
                     /locus_tag="OO357_004162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858243.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5888870.1"
                     /translation="MIYGGFMKSGVQLNLRARESQRILIDAAAEILHKSRTDFILEMA
                     CKAAEDVILDRRVFNFNDRQYEEFIEMLDAPVADDPAIEKLLARKPQWDV"
     gene            1548..>2032
                     /locus_tag="OO357_004163"
     CDS             1548..>2032
                     /locus_tag="OO357_004163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5888871.1"
                     /translation="MGCVTAPEPLSSFHQVAEFVSSEAVLDDWLKQKELKNQAIGATR
                     TFVVCRKGTQQIVGFYSLATGSVNHTEATGNLRRNMPDPIPVIILARLAVDVSFRGKG
                     LGADLLHDAVRRCYRVAENIGVRAIMVHALTENAKQFYIHHGFKPSKTQVQTLFLKLP
                     Q"
BASE COUNT          523 a          427 c          582 g          500 t
ORIGIN      
        1 ggacattatt tttgtggaac cggaggtaac agaccagacg gtttaaatga gccggttact
       61 ttaaacgggt gatggacagc acctcaaaag ttcagatgga ttatatttgt ctgcaatacc
      121 ccgggtattt tcgatttgcg aaatggatgg aacgacttgc tcaggggata gctgatggag
      181 ttactgaagt accgaaggac cactaaaaat cacaggccgg gaatttcggc gtttccggcg
      241 taggtgtgcc acgaatgcat ccagtggcgt gatgttggat gcaatgctgt atgagagatg
      301 aggggcggcg tccagacgca gccttcaaac cgccttgagc tgctgccgtt aagagcggtg
      361 gtggtgagcg tcaaggttaa aaggcgcaga acatgtgtca ggtaggtgtt gcggagatga
      421 acgaaagtga actgtcgatg aagtgtcgaa aggaacctct aggcgatgtc gaaaatgtgc
      481 tatctacgca gcgcatgacg agtccagcag atatctggta ctggctgggc ggcatccggc
      541 atataggcag catgaacgca acacaggctc tcgtacggaa cgtgggaacc tgtcgtctcg
      601 atgctaaggg agcggcgcgg agcagaggca ctgcaagcgc ctgagtaccg atgcgagaca
      661 caggggcaga tggcttcgta gtagtgagga agtgcctgta atgggcatgg agcgaagggg
      721 gtcagtcgtt ggggctggtt tatcggtcaa ccggatagcc gggaggagcc catgagtcag
      781 ccaaagccgt tcgaggtctc taagtatgcg gtttggaaag cataccagcg ggtcaaagct
      841 aatcgaggtg cggcaggtgt cgatgggcag tcagtcgagg cgtttgaggt taccaatcga
      901 agctataccc ggtaatggcg caggttgacc tgcacttggc caagtggatt gcccgcaaac
      961 acaagcgagc gcgaggtagt ctggtgcgtg cattcgagtg gttactgcga attcgtcacg
     1021 actgcccgac gctgtttgca cactggtgct tagcttacga tacttgaatg gttcaacgac
     1081 aagagccgta tgacgcgaga gtgtcacgta cggctccgtg ggagcgtagg ggggcggtgc
     1141 ccctgcgcca cccgatatcc cctttgagtg cggttacgtc cacagaggta aatgccgctg
     1201 acgcggcatc tgtaatctgt tactttgtgt atatcatttg ctatatattg tcaatatgct
     1261 attgttgtaa agcaaatgat atacggaggc tttatgaaat caggtgttca gctcaatctc
     1321 agagcaagag aatcacagcg gatactcatt gatgctgctg cagaaattct tcataagtcg
     1381 cgtacagatt tcattctgga aatggcctgt aaagctgctg aagatgttat tctcgaccgg
     1441 cgggtgttta attttaatga caggcaatat gaagagttca ttgaaatgct tgatgctccg
     1501 gttgcagatg atcctgccat cgaaaaattg ctggcaagga aacctcaatg ggatgtgtaa
     1561 ctgcaccaga acctttatcc tcatttcatc aggtcgctga atttgtcagc agtgaagctg
     1621 tacttgatga ctggttaaag cagaaggagc taaaaaatca ggcaatcgga gccacccgga
     1681 cttttgttgt atgcagaaaa ggtacgcaac aaatagtcgg tttttactca ctggccactg
     1741 gtagtgtcaa tcatacagaa gctacaggta accttcggcg caatatgcca gaccccattc
     1801 ctgtaattat acttgcacgc ctggctgtag atgtgtcatt tcgaggaaaa gggcttggcg
     1861 cggatttact tcatgatgca gttcgtcgtt gttacagggt tgccgaaaat atcggtgtgc
     1921 gtgcaattat ggttcatgcg cttacggaaa acgcaaaaca gttttatatt catcatggtt
     1981 ttaagccatc aaaaactcag gtgcaaacat tgtttcttaa actccctcaa ta
//
LOCUS       DAJTRC010000241         2024 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.251, whole genome shotgun sequence.
ACCESSION   DAJTRC010000241 DAJTRC010000000
VERSION     DAJTRC010000241.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2024)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.251"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..352)
                     /locus_tag="OO357_004164"
     CDS             complement(<1..352)
                     /locus_tag="OO357_004164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000382182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HCR5888872.1"
                     /translation="MDVDVSTSVAGNKPQRIRRIQTVTLVLLFMAGIVNFLDRSSLSV
                     AGEAIRGELGLSATEFGVLLSAFSLSYGFSQLPSGILLDRFGPRIVLGAGLIFWSLMQ
                     ALTGMVNSFSHFILM"
     gene            complement(419..1633)
                     /locus_tag="OO357_004165"
     CDS             complement(419..1633)
                     /locus_tag="OO357_004165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-galactonate dehydratase family protein"
                     /protein_id="HCR5888873.1"
                     /translation="MKIIAADVFVTCPGRNFVTLKITTESGLCGLGDATLNGRELSVA
                     SYLKDHLCPQLIGRDASRIEDIWQFFYKGAYWRRGPVTMSAISAIDMALWDIKAKAAN
                     MPLYQLLGGASREGVMVYCHTTGRTIDEVLEDYAKHQQMGFKAIRVQCGVPGMQTTYG
                     LAKGKGLAYEPATKGLWPEEQLWSSEKYLDFTPKLFEAVRNKFGFNEHLLHDMHHRLT
                     PIEAARFGKSIEDYRLFWMEDPTPAENQECFRLIRQHTVTPIAVGEVFNSIWDCKQLI
                     EEQLIGYIRTTITHAGGITGMRRIADFASLYQVRTGSHGPSDLSPVCHAAALHFDLWV
                     PNFGVQEYMGYSEQMLEVFPYSWRFEEGYMHPGDEPGLGISFDEKLAAKYPYDPEYLP
                     VARLEDGTLLNW"
BASE COUNT          629 a          489 c          412 g          494 t
ORIGIN      
        1 gcattaaaat aaagtggcta aaagaattaa ccattcctgt taatgcctgc attaatgacc
       61 agaatattaa gcctgcgcct aaaactattc gtgggccaaa gcggtctagc aaaataccgg
      121 aaggtagttg tgaaaaacca taagacagag aaaatgcaga aagcaaaaca ccaaactctg
      181 tcgccgataa tcctaactcg ccacgaatcg cttcgcctgc cacgctcaat gacgaacggt
      241 cgagaaaatt aacgatcccc gccataaata ataaaaccag ggtcactgtc tgaatacgac
      301 gaatccgttg cggtttatta cccgcaacac tggttgacac atcaacgtcc atttttagct
      361 ccttgagcgg aattaatcga tttgatgact attcagaaaa taattaagcg aaccgttatt
      421 accagttcaa caatgtgcca tcttccagtc gtgcgacggg gagatactcc gggtcatacg
      481 gatatttcgc cgccagtttc tcatcgaaag aaatacccag ccccggttca tcgcctgggt
      541 gcatataacc ttcctcgaag cgccagctgt acgggaaaac ttcaagcatt tgttcggaat
      601 agcccatgta ctcctgaacg ccaaaatttg gtacccatag gtcaaaatgc agcgccgccg
      661 catgacaaac aggcgataaa tctgaagggc catgcgaacc ggtacgtacc tgataaagcg
      721 atgcaaaatc ggcaatccgc cgcatcccgg taatgccgcc tgcatgggtg atcgtcgtgc
      781 gaatatagcc aatcagctgc tcttcgatta actgcttgca gtcccagata ctgttaaaca
      841 cttcaccgac cgcaatcggc gtcacggtat gctggcgaat cagacgaaaa cattcctggt
      901 tctcggcagg cgtcggatct tccatccaga acaggcggta atcctcaatg ctcttcccaa
      961 agcgcgccgc ttcgatgggg gtcaggcgat ggtgcatgtc atgcaacaga tgttcgttaa
     1021 aaccgaactt attacgcacc gcttcgaata acttcggcgt aaaatcgaga tacttttctg
     1081 aagaccacaa ctgctcttct ggccacaggc ctttggttgc cggttcatac gccagtcctt
     1141 tgcccttcgc caggccataa gtggtttgca tacccggtac accgcactgc acacgaatcg
     1201 ccttaaagcc catctgctgg tgctttgcat agtcttcaag gacttcatca atcgtacgcc
     1261 cggtggtgtg gcaataaacc atcacgcctt cacgagaagc accaccaagc agttgataca
     1321 atggcatatt tgccgcttta gctttgatat cccacaacgc catatcaatg gcagaaatgg
     1381 ctgacatcgt gacggggcca cgtcgccagt aagcaccttt ataaaagaat tgccagatat
     1441 cttcgatacg gctggcatcg cggccaatta gttgtggaca aagatgatct ttcagataag
     1501 aagcgaccga caattcgcga ccatttaacg tggcatctcc taatccacaa agtccgcttt
     1561 cagtggttat tttaagcgta acgaaattac gccctgggca agtaacaaaa acatctgcag
     1621 caataatttt cattattgca atctcctgtg ttgtaaatag acgtttcaaa cctaatttta
     1681 cgcctactac catacaagag agaaaaagcg attattcaac cgtgatcaca ttaaaaacaa
     1741 aaatttctac cgaggagaaa aaataccctc aaaaaccacc tgaataatta accattattg
     1801 ttaaaacaac acgttgaata atcacataac aaactccatt aatcattgaa aatgagcaca
     1861 gaaatagaaa ttaattatat tcaattaatt aaaatgtgta ttgcttgatg tatttaaaaa
     1921 tattattttc atggtgagag tggaggtaaa gaagacacaa taataaaaag gaatgtgaca
     1981 aataatggtt ctggttgggc gttaaattta aggtcaccag aatg
//
LOCUS       DAJTRC010000242         2019 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.252, whole genome shotgun sequence.
ACCESSION   DAJTRC010000242 DAJTRC010000000
VERSION     DAJTRC010000242.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.252"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..621
                     /gene="proB"
                     /locus_tag="OO357_004166"
     CDS             <1..621
                     /gene="proB"
                     /locus_tag="OO357_004166"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5888874.1"
                     /translation="ADKLLLLTDQKGLYTADPRRNPQAELIKDVYGIDDALRAIAGDS
                     VSGLGTGGMSTKLQAADVACRAGIDTIIAAGSKPGVIGDVMEGISVGTLFHAQATPLE
                     NRKRWIFGAPPAGEITVDEGATAAILERGSSLLPKGIKSVTGNFSRGEVIRICNLEGR
                     DIAHGVSRYNSDALRRIAGHHSQEIDAILGYEYGPVAVHRDDMITR"
     gene            633..1886
                     /gene="proA"
                     /locus_tag="OO357_004167"
     CDS             633..1886
                     /gene="proA"
                     /locus_tag="OO357_004167"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HCR5888875.1"
                     /translation="MLEQMGIAAKQASYKLAQLSSREKNRVLEKIADELEAQSEIILN
                     ANAQDVADARANGLSEAMLDRLALTPARLKGIADDVRQVCNLADPVGQVIDGGVLDSG
                     LRLERRRVPLGVIGVIYEARPNVTVDVASLCLKTGNAVILRGGKETCRTNAATVAVIQ
                     DALKSCGLPAGAVQAIDNPDRALVSEMLRMDKYIDMLIPRGGAGLHKLCREQSTIPVI
                     TGGIGVCHIYVDESAEIAEALKVIVNAKTQRPSTCNTVETLLVNKNIADSFLPALSKQ
                     MAESGVTLHADAAALTQLQAGPAKVVAVKAEEYDDEFLSLDLNVKIVSDLDDAIAHIR
                     EHGTQHSDAILTRDMRNAQRFVNEVDSSAVYVNASTRFTDGGQFGLGAEVAVSTQKLH
                     ARGPMGLEALTTYKWIGIGDYTIRA"
BASE COUNT          483 a          509 c          584 g          443 t
ORIGIN      
        1 gccgataaac tgttgctgct gaccgatcaa aaaggtttgt ataccgctga tccgcgcaga
       61 aatccgcagg cagaactgat taaagatgtt tacggcattg atgacgcact gcgcgcgatt
      121 gccggtgaca gcgtttcagg cctcggaact ggcggcatga gtactaaatt gcaggccgct
      181 gacgtggctt gccgtgcggg tatcgacacc attattgccg cgggcagcaa gccgggcgtt
      241 attggtgatg tgatggaagg catttccgtc ggtacgctgt tccatgccca ggcgactccg
      301 cttgaaaacc gtaaacgctg gattttcggt gcgccgccgg cgggtgaaat cacggtagat
      361 gaaggggcaa ctgccgccat tctggaacgc ggcagctccc tgttgccgaa aggcattaaa
      421 agcgtgactg gcaatttctc gcgtggtgaa gtcatccgca tttgcaacct cgaaggtcgc
      481 gatatcgccc acggtgtcag tcgttacaac agcgatgcat tacgccgtat tgccggacac
      541 cactcgcaag aaattgatgc aatactggga tatgaatacg gcccggttgc cgttcaccgt
      601 gatgacatga ttacccgtta aggagctggc cgatgctgga acaaatgggc attgccgcga
      661 agcaagcctc gtataaatta gcgcaactct ccagccgcga aaaaaatcgc gtgctggaaa
      721 aaatcgccga tgaactggaa gcacaaagcg aaatcatcct caacgctaac gcccaggatg
      781 ttgctgacgc gcgagccaat ggccttagcg aagcgatgct tgaccgtctg gcactgacgc
      841 ccgcacggct gaaaggcatt gccgacgatg tacgtcaggt gtgcaacctc gccgatccgg
      901 tggggcaggt aatcgatggc ggcgtactgg acagcggcct gcgtcttgag cgtcgtcgcg
      961 taccgctggg ggttattggc gtgatttatg aagcgcgccc gaacgtgacg gttgatgtcg
     1021 cttcgctgtg cctgaaaacc ggtaatgcgg tgatcctgcg tggtggtaaa gaaacctgtc
     1081 gcactaacgc ggcaacggtg gcggtgattc aggacgccct gaaatcctgt ggcttaccgg
     1141 cgggtgccgt gcaggcgatt gataatcctg accgtgcgct ggtcagtgaa atgctgcgta
     1201 tggataaata catcgacatg ctgatcccgc gtggtggcgc tggtttgcat aaactgtgcc
     1261 gtgaacagtc gacaatcccg gtgatcacag gtggtatagg cgtatgccat atttacgttg
     1321 atgaaagtgc agagatcgct gaagcattaa aagtgatcgt caacgcgaaa actcagcgtc
     1381 cgagcacatg taatacggtt gaaacgttgc tggtgaataa aaacatcgcc gatagcttcc
     1441 tgcccgcatt aagcaaacaa atggcggaaa gcggcgtgac attacacgca gatgcagctg
     1501 cgctgacaca gttgcaggca ggccctgcga aggtggtggc ggttaaagcc gaagagtatg
     1561 acgatgagtt tctgtcatta gatttgaacg tcaaaatcgt cagcgatctt gacgatgcca
     1621 tcgcccatat tcgtgaacac ggcacacaac actccgatgc gatcctgacc cgcgatatgc
     1681 gcaacgccca gcgttttgtt aacgaagtgg attcgtccgc tgtttacgtt aacgcctcta
     1741 cgcgttttac cgacggcggc cagtttggtc tgggtgcgga agtggcggta agcacacaaa
     1801 aactccacgc gcgtggccca atggggctgg aagcactgac cacttacaag tggatcggca
     1861 ttggtgatta caccattcgt gcgtaaataa aaccgggtga tgcaaaagta gccatttgat
     1921 tcacaaggcc attgacgcat cgcccggtta gttttaacct tgtccaccgt gattcacgtt
     1981 cgtgaacatg tcctttcagg gccgatatag ctcagttgg
//
LOCUS       DAJTRC010000243         2004 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.253, whole genome shotgun sequence.
ACCESSION   DAJTRC010000243 DAJTRC010000000
VERSION     DAJTRC010000243.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2004)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.253"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..312
                     /gene="yieF"
                     /locus_tag="OO357_004168"
                     /gene_synonym="chrR"
     CDS             <1..312
                     /gene="yieF"
                     /locus_tag="OO357_004168"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR5888876.1"
                     /translation="SVPGGLKNAIDWLSRLPDQPLAGKPVLIQTSSMGVIGGARCQYH
                     LRQILVFLDAMVMNKPEFMGGVIQNKVDPQTGEVIDQGTLDHLTGQLTAFGEFIQRVK
                     I"
     gene            complement(366..1703)
                     /gene="adeP"
                     /locus_tag="OO357_004169"
     CDS             complement(366..1703)
                     /gene="adeP"
                     /locus_tag="OO357_004169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine permease AdeP"
                     /protein_id="HCR5888877.1"
                     /translation="MSHQHTTQTSGQRMLERVFKLREHGTTARTEVIAGFTTFLTMVY
                     IVFVNPQILGVAGMDTSAVFVTTCLIAAFGSIMMGLFANLPVALAPAMGLNAFFAFVV
                     VQAMGLPWQIGMGAIFWGAVGLLLLTIFRVRYWMIANIPVSLRVGITSGIGLFIGMMG
                     LKNAGVIVANSETLVSIGNLTSHSVLLGILGFFIIAILASRNIHAAVLVSIVVTTLLG
                     WMLGDVHYNGIVSAPPSVMTVVGHVDLAGSFNLGLAGVIFSFMLVNLFDSSGTLIGVT
                     DKAGLADEKGKFPRMKQALYVDSISSVTGSFIGTSSVTAYIESSSGVSVGGRTGLTAV
                     VVGLLFLLVIFLSPLAGMVPDYAAAGALIYVGVLMTSSLARVNWQDLTESVPAFITAV
                     MMPFSFSITEGIALGFISYCVMKIGTGRLRDLSPCVIIVALLFILKIVFIDAH"
     gene            1868..>2004
                     /locus_tag="OO357_004170"
     CDS             1868..>2004
                     /locus_tag="OO357_004170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418171.1"
                     /note="YieH; catalyzes the dephosphorylation of
                     phosphoenolpyruvate, AMP and p-nitrophenyl phosphate;
                     purine and pyrimidine nucleotides are secondary
                     substrates; member of the haloacid dehalogenase-like
                     hydrolases superfamily; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconate phosphatase"
                     /protein_id="HCR5888878.1"
                     /translation="MSRIEAVFFDCDGTLVDSEVICSRAYVTMFQEFGITLDPEEVFK
                     R"
BASE COUNT          578 a          528 c          495 g          403 t
ORIGIN      
        1 tcggtaccgg gtgggctgaa aaatgccatc gactggcttt cccgcctgcc ggatcaaccg
       61 ctggccggta aaccggtgtt gattcagacc agctcaatgg gcgtgattgg cggcgcgcgc
      121 tgtcagtatc acctgcgcca gattctggtt ttcctcgatg caatggtgat gaacaagccg
      181 gaatttatgg gcggcgtgat tcagaacaaa gttgatccgc aaaccggaga agtgattgat
      241 cagggtacgc tggaccacct gaccgggcaa ttgaccgcat ttggtgagtt tattcagcga
      301 gttaagatct aaataaaaaa cccgccagta atcatgcatg gcgggttttt aatgcgctat
      361 cgattttaat gagcgtcgat aaatacaatc ttcaggataa acagcagcgc aacgatgatt
      421 acgcacgggc taaggtcacg cagacgcccg gtaccaatct tcatcacgca gtaggagata
      481 aagcccagcg caataccttc ggtaatcgaa aagctgaacg gcatcatcac ggcggtaata
      541 aacgccggaa cagattcagt aagatcctgc cagttcacgc gagcaagact tgaggtcatc
      601 aacacgccaa cgtagatcaa cgcgccagct gcagcgtagt ctggcaccat tcccgccagc
      661 ggcgacagaa agataaccag caggaacagc agaccaacaa ccactgccgt cagaccggta
      721 cgaccgccaa ccgatacgcc ggaagaggac tcaatataag ccgtaacgga agaagtaccg
      781 ataaacgaac cggtcacgga agagatactg tcgacataca gcgcctgctt catgcgcggg
      841 aatttcccct tctcatccgc cagacctgct ttatcggtca cgccaatcag cgtaccggag
      901 gagtcaaaca agttgaccaa catgaaagag aaaatcaccc ctgccagccc gaggttaaac
      961 gacccggcta aatctacatg acccacaact gtcattacgc tcggcggcgc agaaacgatg
     1021 ccattgtagt gcacatcacc cagcatccag cccagcagcg tcgtcaccac gatagaaacc
     1081 agcaccgctg cgtgaatgtt gcgcgaggcc agaatagcaa tgatgaaaaa gccgaggata
     1141 cccagaagta cgctgtgaga agtcagatta ccgatgctca ccagcgtttc cgagttagcg
     1201 acaatcacac ctgcgttttt cagccccatc atgccgataa acaggccgat accgctggta
     1261 atacccacac gcagacttac cggaatgtta gcaatcatcc agtaacgaac gcggaaaatg
     1321 gtcaaaagaa gcagtccgac agcgccccag aagattgcgc ccataccgat ctgccacggc
     1381 agccccattg cctggacaac cacaaaagcg aaaaaggcgt taagccccat cgcaggagcc
     1441 agcgccaccg gaaggtttgc aaacaatccc atcataatgc taccgaaagc agcaatcaga
     1501 caggttgtta cgaagacggc actggtgtcc atgccagcaa cgcccagaat ttgtgggtta
     1561 acaaatacga tgtagaccat cgtcaggaag gtggtgaacc cggcaatcac ttcagttcgt
     1621 gccgtagtac catgttcgcg cagtttaaaa acacgttcca gcatcctctg accagaagtc
     1681 tgggtggtat gttgatgact cattatctat ttccgaacaa ggagggaaaa tccgtcgcta
     1741 tcgtatacca aaatgcgaca ataggcgcgt ttgtgagaga cttttttatt ggatttactt
     1801 atacggcaac gattgcgttg cgcaaatcgg ctctacgaaa acgttaaact gattaaaaag
     1861 gaaaggcatg tcccggatag aagcggtatt tttcgactgc gacggtacgc tggtcgacag
     1921 tgaagtcatt tgctcccgcg catatgtaac gatgtttcag gaatttggta ttacgctcga
     1981 tcctgaagag gtattcaaac gttt
//
LOCUS       DAJTRC010000244         1921 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.254, whole genome shotgun sequence.
ACCESSION   DAJTRC010000244 DAJTRC010000000
VERSION     DAJTRC010000244.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.254"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(30..1181)
                     /locus_tag="OO357_004171"
                     /pseudo
     CDS             complement(30..1181)
                     /locus_tag="OO357_004171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612091.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            1246..1894
                     /locus_tag="OO357_004172"
                     /pseudo
     CDS             1246..1894
                     /locus_tag="OO357_004172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000164328.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          478 a          482 c          422 g          539 t
ORIGIN      
        1 gatcctaccc acgtaatgtg gacacggccc taagcgagat tatggttttc aaattgttcc
       61 gggctgagac cgccacaggc actgtgacga cgccagcgat tgtaatcaca ctcgatataa
      121 ttaaacaccg ttgcccgcat tatttcccgg ctgctaaagc gttccccgtg gatacattcc
      181 accttcagcg aatgaaagaa gctttccaca caggcattgt cataacagta gcctttcgca
      241 ctcatactgc cacgcaggtt gtgtcgcttc agcattgcct gataatcccc tgaacagtat
      301 tgaccaccac ggtccgtatg aacaatgacg ctttccgggc gtcttctccg ccacaacgcc
      361 atttgcagtg catcacaggc cagttgtgct gtcattcgcg gtgacatcga ccagccaatg
      421 acggcgcgtg accacaggtc gatgactact gcgagataca accagccctc atcggtacgc
      481 aagtacgtga tgtcacccgc ccacttctgg tttcgggcca ctggcgctga agtcctgctc
      541 cagcagattc tccaatacgg gcaggccatg tgcacggtag ctgaccgggc tgaacttccg
      601 gctggctttc gcccgcagcc cctgacgacg caggctggcg gcaatggttt taatattgaa
      661 ctccggcagt tcgtcagcaa ggcggggagc accgtatcgc tgctttgcct caaagaatgc
      721 cttatggaca gcggcatcgc aggtgagccg aaactgttgg cgcaggctca tctggtgacg
      781 acgcctgagc cagacatacc agccgctgcg ggcaacccga agtacacgac acatcgcttt
      841 gatgctgaac tctgcccgat gattttcgat aaagacatac ttcatttcag gcgcttcgcg
      901 aagtatgtcg cggccttttg gaggatggac agttcctcag cctgctccgc cagttgtcgt
      961 ttaaggcgga cattttcagc ggccagttcg ctttcgcgct ctgacgaact catttgttgc
     1021 tgctgtttac tgcgccaggc ataaagctga gattcataca ggctgagttc acgggctgcg
     1081 gcggccacac cgatgcgttc agcgagtttc agggcttcgt tacgaaattc aggcgtatgt
     1141 tgtttacggg gcttcttgct gattgatact ggttttgtca tgagtcacct ctggttgaga
     1201 gtttactcac ttagtcctgt gtccactatt ggtgggtaag atcaccctcc attaaaacta
     1261 aaacgggcgc tttctgatat atcatctgta agcgcagtaa taccattagt agcacctcgc
     1321 gcattgatta ttgaatatta taactggaat gttatatttc ttgttatgat gctgttcagt
     1381 attataataa tgttattatc accaaagata actcatgcaa atgagaaagt aatatgtcaa
     1441 aacagtgcat cctttgtgac caaagagttt atctgcggca ctatacttgt ctcattaata
     1501 ctcagttctc tattttgcta catatcaata tcaccatcaa ttttcatgca tacttatggc
     1561 ctgagtactt ttagttactc aattattttc tccttctcag ttttattttt cataatcgga
     1621 aatcaattat caaagattga aaaattatcc aacctatggt ttttattacc caatagccaa
     1681 ttttatcgca ttacaaataa atggttcaaa aacaggattt gccgcatcta ttacaggttt
     1741 tactcaaaca ataagcgctg gtacaatcag tttcctgtca gtaaccgtaa gcgacaacgg
     1801 tgttggtccc ggaactactt taggtcttgg ttcactaata acttcattac tgtcaatagc
     1861 cgtaatcgcc attgcaggaa ataataaaaa gtaagtgtac tctgactgcg tatgtatcta
     1921 c
//
LOCUS       DAJTRC010000245         1903 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.255, whole genome shotgun sequence.
ACCESSION   DAJTRC010000245 DAJTRC010000000
VERSION     DAJTRC010000245.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1903)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1903)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1903
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.255"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(114..1568)
                     /gene="ospC2"
                     /locus_tag="OO357_004173"
     CDS             complement(114..1568)
                     /gene="ospC2"
                     /locus_tag="OO357_004173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC2"
                     /protein_id="HCR5888879.1"
                     /translation="MKIPEAVNHINVQNNIDLVDGKINPNKDTKALQKNISCVTNSSS
                     SGISEKHLDHCADTVKSFLRKSIAAQSYSKMFSQGTSFKSLNLSIEAPSGARSSFRSL
                     EHLDKVSRHYLSEIIQKTHPLSSDERHLLSIIINSDFNFRHQSNANLSNNTLNIKSFD
                     KIKSENIQTYKNTFSEDIEEIANHDFVFFGVEISNHQETLPLNKTHHTVDFGANAYII
                     DHDSPYGYMTLTDHFDNAIPPVFYHEHQSFFLDNFKEVVDEVSRYVHGNQGKTDVPIF
                     NTKDMRLGIGLHLIDFIRKSKDQRFREFCYNKNIDPVSLDRIINFVFQLEYHIPRMLS
                     TDNFKKIRLRDISLEDAIKASNYEEINNKVTDKKMAHQALAYSLGNAKSDMALYLLSK
                     FNFTKQDIAEMEKMNNNMYCELYDVEYLLSEDSANYKVLEYFISNGLVDVNKRFQKAN
                     SGDTMLDNAMKSKDSKTIDFLLKNGAVSGKRFGR"
BASE COUNT          608 a          325 c          293 g          677 t
ORIGIN      
        1 tcaccacatg caatgtgttg ttgcagggga acggtctgca ccatccccta tatcgatcaa
       61 tataacaaaa atgacaacgt cagattcagg gcacaaccta aatatatttt aggtcacctc
      121 ccaaatcgtt tgcctgatac cgctccattt tttaataaaa aatcaattgt ttttgaatct
      181 ttgcttttca ttgcattgtc aagcatagtg tccccactat ttgctttttg aaatcttttg
      241 tttacatcaa ccaatccatt actaataaaa tattctagca ctttatagtt ggcactatct
      301 tcactaagta aatactcaac atcatacagc tcacaataca tattgttgtt cattttctcc
      361 atctctgcga tatcttgttt tgtaaaatta aatttagata gtaaataaag tgccatatca
      421 gattttgcat tgcctagaga ataagcaaga gcttgatgag ccattttttt atcagtcacc
      481 ttattgttaa tttcttcata attggatgct ttaatggcat cctccaaaga tatatctctg
      541 agtcttatct ttttgaagtt atctgtgctt agcattcttg gtatatgata ctcaagctga
      601 aacacaaagt ttataattct atccagacta acaggatcaa tgtttttatt ataacaaaac
      661 tctcggaatc tttgatcttt actctttctt ataaaatcaa taagatgcaa cccaattcca
      721 agcctcatat ctttagtatt aaatattggc acatcagttt ttccctgatt accatgcaca
      781 tacctgctaa cttcatccac aacctcttta aagttatcta agaaaaatga ttggtgctca
      841 tggtaaaaaa caggtggaat agcattatca aagtggtcgg ttaatgtcat atatccatat
      901 ggagagtcat gatcaatgat atacgcatta gcaccaaaat ccacagtatg atgtgtttta
      961 tttagtggga gtgtttcttg atgattggaa atttcaaccc caaaaaaaac aaaatcgtgg
     1021 ttagctattt cttcaatatc ctcagagaat gtgtttttat atgtttgtat attctcagac
     1081 ttaattttat caaaggactt aatattaaga gtattatttg acaggttagc gttactttga
     1141 tgtctgaagt taaaatcaga attaataata atagagagca agtgacgttc atccgaagaa
     1201 agaggatgag ttttttgtat tatttcagaa agatagtgtc tggacacctt atcgaggtgc
     1261 tcaagtgacc gaaatgatga ccgtgctcca gatggcgctt caatagaaag atttaatgat
     1321 ttgaaactgg tcccttgaga gaacatttta ctataacttt gcgctgctat agacttcctt
     1381 aaaaagcttt tcacagtatc agcacagtga tctaaatgtt tttcacttat gccagaagag
     1441 gatgagtttg ttacgcatga tatgtttttt tgtaatgctt ttgtatcttt gttcggattt
     1501 atttttccat caacaagatc aatattgttc tgaacattaa tatgatttac tgcttcaggt
     1561 attttcataa aacatccccc attatgtgcg tttttatctg cagttattta gaataatatt
     1621 atctaactta atattttctt gtatgtatta taacgaacat attttaatat gttgtacgat
     1681 tttcactctg aaatcccctg agtttaatca cacttccttt actgaaagga attattgcaa
     1741 cagtattatt tgtttttgga tatatatctt gcgccttgtt atgtagttaa tgctcagaat
     1801 atgatagata aaatagagac gcaaaacagg gcatgctcat aacatgatga taacataaaa
     1861 aattagtaac aataaatgaa agaatcatgg gcgctcaaca aaa
//
LOCUS       DAJTRC010000246         1893 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.256, whole genome shotgun sequence.
ACCESSION   DAJTRC010000246 DAJTRC010000000
VERSION     DAJTRC010000246.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1893)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1893
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.256"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            190..374
                     /locus_tag="OO357_004174"
                     /pseudo
     CDS             190..374
                     /locus_tag="OO357_004174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001775038.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
     gene            517..738
                     /locus_tag="OO357_004175"
                     /pseudo
     CDS             517..738
                     /locus_tag="OO357_004175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(759..821)
                     /locus_tag="OO357_004176"
                     /pseudo
     CDS             complement(759..821)
                     /locus_tag="OO357_004176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001743492.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
     gene            1271..>1893
                     /gene="ospC1"
                     /locus_tag="OO357_004177"
     CDS             1271..>1893
                     /gene="ospC1"
                     /locus_tag="OO357_004177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 3 secretion system effector OspC1"
                     /protein_id="HCR5888880.1"
                     /translation="MNISETLNSANTQCNIDSMDNRLHTLFPKVTSVRNAAQQTMPDE
                     KNLKDSANIIKSFFRKTIAAQSYSRMFSQGSNFKSLNIAIDAPSDAKASFKAIEHLDR
                     LSKHYISEIREKLHPLSAEELNLLSLIINSDLIFRHQSNSDLSDKILNIKSFNKIQSE
                     GICTKRNTYADDIKKIANHDFVFFGVEISNHQKKHPLNTKHHTVDFGA"
BASE COUNT          647 a          376 c          352 g          518 t
ORIGIN      
        1 gtcaaaaaaa tcatcttcgt gaagcttcac gacatagcgg ggaagtttgc ttctttgaga
       61 ggcgggttta cgtttacggg atttagctga acgggccata taaccacctg aaagacaatg
      121 acattgcctg tttttataac ggtaattgca gaccatgaca aaccgcagcc gtcaggctgc
      181 ctactcgagt cacaaggctc tttgtgtccg atatgccggc agcgcattac ccatgaaact
      241 ggctggaacg tacaccataa ggtgaagaaa gttatggggg cggtgaagaa ttatctaacc
      301 ttgtcttgtt acatccaaat tgccatcgac agttacacag ttgtgaagcc ggctctctca
      361 aaaagggctt ataaaggctt gagcggtgta ccgggaaacc ggtatgcacc gttcttaggg
      421 gagggcagtc cagcaatgaa ctgtccttac ccgacaaaca tccaaaacga acggaacgtg
      481 ctggaatccg catataaccc gttgtaaagt gaggaaatgg acacctcact tgctcatgag
      541 aacgcccgcc tgcgggcact gttgcagacg caacaggaca ctatccacca gatggctgaa
      601 tacaaccgcc tgctctcaca gcggatggcg gcttatgctt ccgaaatcaa ccgactgaag
      661 gcgctggttg cgaaactgca gcgtatgcag ttcggtaaaa gctcagaaaa acttcgcgca
      721 aaaaccgaac ggcagattcc attttcacgg gcgatctacg ccacacaggc tctgggttgc
      781 agtgactgct ccattaaggc gattttgaac tcataaggga agtaacaaaa aaactgcaaa
      841 agtacaataa ttatatatgg ccaaactaac ctctattaaa aaacatcagt ttttcacact
      901 tagttgagct tcatgaaaaa atcatcgagg atttcattgc agaatacaca ttccactaac
      961 tggaagcact ccataagagg aatcagagaa agtatcgttt ttttatagta aaattcattg
     1021 ctgttcaata aaattgatat agttattaaa ttatgaaaat aatttcacaa aaaagattaa
     1081 cttgagttct taagcatcgg attttagata ttttgaaata tctggcatca cataacatca
     1141 aaagtatata atactcttat ctaattacat gcagtatatt gtatatatac tcaaaaattg
     1201 taattcatac cctgctatat cttgtaggga aagatattaa ttaaaactgt tttcatataa
     1261 ggttcatttt atgaatatat cagaaacact gaactcagca aatacccaat gcaatataga
     1321 ttctatggat aacagattac atacattgtt tccaaaagtg acatcagtgc gaaacgctgc
     1381 acaacaaact atgccagatg aaaaaaattt aaaagatagt gcaaatatta ttaaaagttt
     1441 ctttaggaaa actatagcag cacagagtta tagtagaatg ttctctcaag gctctaactt
     1501 taaatcttta aatatagcaa ttgatgcacc atcagacgct aaagcctcat ttaaggctat
     1561 tgagcacctt gacagattat cgaagcatta tatatctgaa ataagggaaa aacttcatcc
     1621 tctttctgca gaggaactca atttgctttc gctaattatt aattctgatt taatcttcag
     1681 acatcaaagt aattctgatt tgtctgataa aattttaaac attaagtcat tcaataaaat
     1741 tcagtctgaa ggaatatgca caaaacgaaa cacatacgct gatgatataa aaaaaatagc
     1801 taatcatgac tttgtgtttt ttggcgttga aatctctaac catcagaaaa aacaccccct
     1861 gaatacaaaa catcacactg ttgattttgg tgc
//
LOCUS       DAJTRC010000247         1826 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.257, whole genome shotgun sequence.
ACCESSION   DAJTRC010000247 DAJTRC010000000
VERSION     DAJTRC010000247.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1826
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.257"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(208..609)
                     /locus_tag="OO357_004178"
                     /pseudo
     CDS             complement(208..609)
                     /locus_tag="OO357_004178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
     gene            1038..1826
                     /gene="virF"
                     /locus_tag="OO357_004179"
     CDS             1038..1826
                     /gene="virF"
                     /locus_tag="OO357_004179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858179.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family invasion system transcriptional
                     regulator VirF"
                     /protein_id="HCR5888881.1"
                     /translation="MMDMGHKNKIDIKVRLHNYIILYAKRCSMTVSSGNETLTIDEGQ
                     IAFIERNIQINVSIKKSDSINPFEIISLDRNLLLSIIRIMEPIYSFQHSYSEEKRGLN
                     KKIFLLSEEEVSIDLFKSIKEMPFGKRKIYSLACLLSAVSDEEALYTSISIASSLSFS
                     DQIRKIVEKNIEKRWRLSDISNNLNLSEIAVRKRLESEKLTFQQILLDIRMHHAAKLL
                     LNSQSYINDVSRLIGISSPSYFIRKFNEYYGITPKKFYLYHKKF"
BASE COUNT          571 a          330 c          339 g          586 t
ORIGIN      
        1 caagggaata tagtttgtgt aagcatattg cttccttatg ttatatttta gtaaagaatt
       61 tattttttac attatagtcg gctaaatgaa gtttactgta aaaaaacgat acttttttcg
      121 taagcgatga gatgtggagc cagaaaaatg gctgcgttac gtcattgaac tgtccttgac
      181 ccacgatatt cagacagtcg ttgtatctta agttgacatt acccgctgac gtcgatattc
      241 ccttggtgag agataaccca acgcactatg cggatggttt tcattgtaat gctcgatcgc
      301 cactgcaagg ttatgcaatg ccgttcttgg tttcggcatg aacgcgatgc agtcttcctt
      361 catcgttttc atgaaccgtt ctgctattcc attactctga gggctactga ctgccgtcgt
      421 gcagggctcc agattcaact ctctggcgaa ctgccgtgtt tcatgagccg tataagccga
      481 accgttatcg gccactggat tggccgttcc ggtcctttat cgccatatcg ctcctgacat
      541 gacatcctgc accgttgctt tgtcataatc tcccgtgcct gctgcccagt ctatggcttc
      601 acgatcgcaa tatggaacgt aacgctgtca tcgggagaat cgtcaatgcg gcattcggtt
      661 aacgcttacc aatatcaaca ccaaccggat ttgtggagcc tccagtctga aggggcttta
      721 tgcgttccgt ataggatatt atgatgctgg agttttgcaa agctccactc ctttccgaac
      781 atttacacag atattgccta agaaaagtga aaccataata tggttatagt ccctttcagt
      841 gcaaatactt agcttgttgc acagagaaat agaagctgca taagctcttt cttcaaaaaa
      901 tgtaaataaa gttaaatata ggaaaaatta cttaatctat cttaataacg gaaagatttt
      961 gtatacaatc actgttacag aaatttctta gttactctgt aaacactaaa tatagtttgg
     1021 tatattctgt tgaatttatg atggatatgg gacataaaaa caaaatagat ataaaggttc
     1081 gcttgcataa ctatattatt ttatatgcaa aaaggtgttc aatgacggtt agctcaggca
     1141 atgaaacttt gactatcgat gaagggcaaa ttgcttttat agagcgaaat atacaaataa
     1201 acgtctccat aaaaaaatct gatagcatta atccatttga gattataagc cttgacagaa
     1261 atttattatt aagcattatt agaataatgg aaccaattta ttcatttcaa cactcctatt
     1321 ctgaggagaa aagggggtta aacaaaaaaa tattcctcct ctctgaggag gaggtttcta
     1381 tcgatttgtt caaatctata aaagagatgc ctttcggcaa aagaaagatc tatagtttag
     1441 cttgcctttt atcagctgtt tctgatgagg aagctttata tacttcgata tcgatagctt
     1501 cttctcttag tttttctgat cagataagga agattgttga aaaaaacatc gagaagagat
     1561 ggcgtctttc tgatatttca aataacttga atttatcaga aatagctgtt agaaaacgat
     1621 tggagagtga aaaattaaca tttcaacaaa tccttcttga tattcgcatg catcatgcag
     1681 caaagctttt attgaatagt caaagctata ttaatgatgt atcaagactt atcggaatat
     1741 caagcccatc ttattttata aggaaattta atgaatatta tggtataact ccaaagaaat
     1801 tttacttata tcataaaaaa ttttaa
//
LOCUS       DAJTRC010000248         1717 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.258, whole genome shotgun sequence.
ACCESSION   DAJTRC010000248 DAJTRC010000000
VERSION     DAJTRC010000248.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1717
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.258"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(110..577)
                     /locus_tag="OO357_004180"
     CDS             complement(110..577)
                     /locus_tag="OO357_004180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HCR5888882.1"
                     /translation="MYGTWSLSMAAIWLLRVDLLFVALFLIFISWGDILLRIISHRYL
                     LVLTVLIFLALILQHQKPNLVAAGSVLLVGFFLFSAGIIGGGDVKLLTVLSLAIDEHE
                     LANFLVAMTFCGALVVLAGLLFFRKSIRENGVPYAVPISLAFLLTYPVFPLFC"
     gene            complement(643..882)
                     /locus_tag="OO357_004181"
     CDS             complement(643..882)
                     /locus_tag="OO357_004181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HCR5888883.1"
                     /translation="MNTFITKYYGKTKQCFARFAKDERGVTAIEYALIGVAMATLLAF
                     IFGDQNSGFLGAIKDAFDAIAAAIQQVTISGTSNP"
     gene            complement(1017..1622)
                     /locus_tag="OO357_004182"
     CDS             complement(1017..1622)
                     /locus_tag="OO357_004182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HCR5888884.1"
                     /translation="MSDDRFYFSLGQGVKFYPASRRITTSTGNVINLSENGYRFLLLL
                     LQGESDKQHIINEVWREQKGSVSDSSYYGQIYMLRKALDVAGLSSSLIKTIPRKGVRY
                     LGKVEKHVCENERDSDEDSSSYTNDSEAREVNTDANIPENDNSQEFSTEELLPIKHTD
                     KNIEWYRTKQWSVFISVLTVLAVCWLTTLVFLVLLFLNQDK"
BASE COUNT          549 a          378 c          305 g          485 t
ORIGIN      
        1 gcttaggcct gtgtccatat tacgtgggta ggatcacaag acctactgct atcacaatta
       61 ctgagagaaa aaaaagcatc cgatgattca tttatgctgt cctaaaaaat taacaaaaca
      121 atgggaaaac aggatatgta agcagaaaag ccaatgaaat tggtacagca taaggcacac
      181 cgttttcacg aattgatttt cgaaaaaaaa gaagacctgc cagaacaacc agagcgccgc
      241 aaaaggtcat tgcgacgaga aaattagcca gttcatgttc atcaatagct aacgaaagaa
      301 cagttaacag cttgacatca ccgccgccaa ttatccctgc ggaaaaaaga aagaagccca
      361 cgagcaaaac gctcccggct gcaaccagat tgggtttttg atgctgtaaa atgagtgcca
      421 aaaaaataag cacggttaac accagtaaat aacgatggct aataattctg agtaatatat
      481 ccccccatga aataaaaata agaaagagcg caacaaataa aagatcgact cttagtaacc
      541 atattgctgc cattgaaaga ctccatgtac catacaaggt tgctgccaga catattcctt
      601 tacgtctgac agcctctaga gagttttttt attttttata agttacggat tactcgttcc
      661 ggaaatcgtt acctgttgaa ttgctgcagc gatagcgtcg aatgcatctt taattgcgcc
      721 caggaagcct gagttctgat cgccaaagat gaaagcaagc agagttgcca tagcaacacc
      781 aatcaatgca tattcgattg cagtaacacc acgttcatcc ttcgcgaaac gcgcaaagca
      841 ctgtttagtt ttaccatagt acttagtaat aaaagtattc atgttttcct cgtatattat
      901 tatttaaatt caaaccacca gcttcatctg cgtgtcaccg gtatcccata tttttgggat
      961 gtggctataa ttatctattc ccgctattta gtcttcaaaa aaaattacat ttaatatcat
     1021 ttatcttgat taagaaatag taagactaaa aaaacaagcg tcgtcagcca gcacactgct
     1081 aacacggtaa gaacgctaat aaaaacactc cactgcttcg ttctatacca ttcaatattt
     1141 ttgtcagtat gttttattgg gagcagctct tcagtagaga attcctggct gttatcattc
     1201 tcaggaatgt ttgcatcagt gtttacttcc ctggcctcgc tatcgttggt atagctacta
     1261 ctgtcttcat cactgtcacg ctcattctca cagacatgtt tctcgacctt tcccagatat
     1321 ctgacgcctt ttcgcggaat ggttttaatt agagaactcg acaaacctgc gacgtctaat
     1381 gcctttctaa gcatgtaaat ctgcccataa taactgctgt cgcttaccga acctttctgc
     1441 tcccgccaaa cctcattgat aatatgttgc ttatcggatt ctccctgtag taaaaggagt
     1501 aaaaatcgat agccgttttc agacagattg attacattgc ctgtactggt ggtgatacgt
     1561 cgagacgctg ggtaaaactt aactccctgc cctaaagaaa aataaaaacg gtcatcgctc
     1621 ataagtaccg acccctgttt tcttcttcac atgactactc attacaactt tgtatgaagt
     1681 aagatgcaaa atcatattgt ttacattaag ttaagca
//
LOCUS       DAJTRC010000249         1716 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.259, whole genome shotgun sequence.
ACCESSION   DAJTRC010000249 DAJTRC010000000
VERSION     DAJTRC010000249.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1716
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.259"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..145)
                     /locus_tag="OO357_004183"
     CDS             complement(<1..145)
                     /locus_tag="OO357_004183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate dehydrogenase"
                     /protein_id="HCR5888885.1"
                     /translation="MDQTYSLESFLNHVQKRDPNQTEFAQAVREVMTTLWPFLEQNPK
                     YRQM"
     gene            381..653
                     /locus_tag="OO357_004184"
     CDS             381..653
                     /locus_tag="OO357_004184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YnjH family protein"
                     /protein_id="HCR5888886.1"
                     /translation="MSRALFAVVLAFPLIALANPHYRPDVEVNVPPEVFSSGGQSAQP
                     CTQCCVYQDQNYSEGAVIKAEGILLQCQRDDKTLSTNPLLWRRVKP"
     gene            complement(619..1026)
                     /gene="nudG"
                     /locus_tag="OO357_004185"
     CDS             complement(619..1026)
                     /gene="nudG"
                     /locus_tag="OO357_004185"
                     /EC_number="3.6.1.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP pyrophosphohydrolase"
                     /protein_id="HCR5888887.1"
                     /translation="MKMIEVVAAIIERDGKILLAQRPAQSDQAGLWEFAGGKVELDES
                     QQQALVRELNEELDIEATVGEYVASHQREVSGRIIHLHAWHVPDFHGTLQAHEHQALV
                     WCSPEEALQYPLAPADIPLLEAFMALRAARAAD"
     gene            1113..>1716
                     /locus_tag="OO357_004186"
     CDS             1113..>1716
                     /locus_tag="OO357_004186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HCR5888888.1"
                     /translation="MLDRHLHPRIKPLLHQCVRVLDKPGITPDGLTLVGFAIGVLALP
                     FLALGWYLAALVVILLNRLLDGLDGALARRRGLTDAGGFLDISLDFLFYALVPFGFIL
                     AAPEQNALAGGWLLFAFIGTGSSFLAFAALAAKHQIDNPGYAHKSFYYLGGLTEGTET
                     ILLFVLGCLFPAWFAWFAWIFGALCWMTTFTRVWSGYLTLK"
BASE COUNT          397 a          400 c          448 g          471 t
ORIGIN      
        1 acatctggcg atattttgga ttttgttcaa gaaaaggcca gagtgtggtc attacttcac
       61 gaacggcttg cgcgaactcg gtttgattcg ggtcgcgctt ttggacatgg ttgaggaatg
      121 actccagaga atatgtctga tccatagata taaaaacctt atatattaat acgattgtgc
      181 ttatgttgtt tatgtcatgt gcttttgcag ttttcgacta taccatcaag aatgtgacgg
      241 taagcaagaa aaaaatgacc caggaaagca aattaataac gagagtaatc tcataaccaa
      301 atgcgataac tcagacgcta aagttttgcc acccggctac cgttatcagt agcagagaca
      361 ataacgttaa ggagttccct gtgagtcgag cattgttcgc cgtagtatta gcatttccat
      421 taattgcact ggctaacccg cattatcgtc cggacgtgga ggtaaacgta ccgccagaag
      481 tgttcagttc aggcgggcag tcagcgcagc cctgtactca gtgctgcgtt tatcaggatc
      541 aaaattattc tgaaggggcg gtaattaaag ccgaaggaat actgctgcaa tgccagcgtg
      601 atgacaaaac ccttagcact aatccgctgc tctggcggcg cgtaaagcca taaacgcctc
      661 taataatgga atgtcagcag gggccagcgg atattgcagc gcctcttcag gtgagcacca
      721 gaccagcgcc tgatgttcat gtgcctgtaa cgtcccgtgg aagtcgggta cgtgccaggc
      781 atgaagatgg ataatccggc ccgaaacttc tcgctgatgg ctggcaacat actcacccac
      841 agttgcttcg atgtccaatt cttcgtttaa ctcacgcacc agcgcctgct gctggctttc
      901 atccagctcg actttacctc cggcaaactc ccataatccc gcctgatcgc tctgggcggg
      961 gcgttgcgcg agtaaaattt tgccatcacg ttcaatgatg gcggcaacaa cttcaatcat
     1021 tttcataaat tacacacggg cagataagcg aaaaatgcca taatatccgc actgttgctc
     1081 aatcagaaag gctttatatt taaggagtgc ctgtgctaga ccgccatctt catccccgga
     1141 ttaaaccgtt gttgcatcag tgcgtgcggg ttcttgataa accgggcatt acgccagatg
     1201 gtttaacatt agtgggattt gccatcggcg tgctggctct gccgtttctg gcgctgggct
     1261 ggtatctggc ggcgctagtc gtcattttgt tgaacaggct gcttgatggt ctggacggtg
     1321 cgctggcgcg gcgcagaggg cttaccgacg cgggcggctt tcttgatatt tctctcgatt
     1381 ttctctttta cgcgctggtg ccgtttggct ttattcttgc tgcaccggag caaaatgcgc
     1441 tggcaggcgg ctggttgttg tttgcgttta tcggcacggg cagcagtttt ctcgcctttg
     1501 ctgcactggc agcaaaacat cagattgata accccggtta tgcacataag tcgttttact
     1561 atctgggggg attaaccgaa ggcacagaaa cgatcttact gtttgtgctg ggatgtttat
     1621 tccctgcatg gtttgcgtgg tttgcgtgga tcttcggtgc gttgtgctgg atgacgacct
     1681 ttacgcgggt gtggagtggg tatctgacgc tgaagt
//
LOCUS       DAJTRC010000250         1714 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.260, whole genome shotgun sequence.
ACCESSION   DAJTRC010000250 DAJTRC010000000
VERSION     DAJTRC010000250.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.260"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(88..1559)
                     /gene="yeeO"
                     /locus_tag="OO357_004187"
                     /pseudo
     CDS             complement(88..1559)
                     /gene="yeeO"
                     /locus_tag="OO357_004187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001323172.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="toxic metabolite efflux MATE transporter YeeO"
BASE COUNT          535 a          465 c          353 g          361 t
ORIGIN      
        1 ggcgaatatt agcgatgccc acccacaatg tcaaagcctg ttttttaaat ttgaaatcgt
       61 ttgctgaaat aatctgcatt ttgtcgttta ttccgacaca actggctttt tttcacactt
      121 ttgcggctcg gatcgagggt atttccacag ccaacgtcca gtaaccattc gccagtaaaa
      181 cagcacggcc cgcacagccc agtcggcaaa cattcccatc cagacaccaa ccacacccca
      241 gccaagcatg attccctgca cataaccgac tacaacccgg caaccccaca tgctcaacat
      301 agaaacccac atggcgtaac gggcatcacg agcaccctta aatccagcgg gtagcaccca
      361 tgaggcggac caaataggca taaataaagc atttagctaa atcagaatca caacgacgtg
      421 tttaacctgt ggatcctggg tgtaaaacga tgccataacc ccggcaaagg gagccgttag
      481 ccaggcgatg gccgttaatc caagagtgga cagccagaac acatgccgca actgaatctc
      541 tgcttgcgct atctgcccta cccccaacct tcggcctgta atgatcgtag aagcagaacc
      601 gagcgcactt ccgggtaagt tgataagagc cgcaattgag aacgcgataa aatttccggc
      661 aataacgctg gtccccatcc cggcaacgaa catttgggtt aataaccgac cactggtaaa
      721 taacactgat tcgacactcg ctggaatacc aatccccatg acttcccaga taatgctaaa
      781 attcagcggt ttaaaatagc tctttaacga aatccttagc gcaggattaa aaccaatcgc
      841 cagcacccac aaaattgcaa ctgcgccaat ataacgagaa atggttaaac ccaacccagc
      901 cccgaccagg agaaaaggcc gtaaatcaat atgccgctaa taataatatt aagaatattc
      961 aggctaccgt taatcagtag cggtattttc gtattccctg caccacgaag tgccccgcta
     1021 ccaataagag tgatggcagc ggctggataa ctgagtacgg tcagctccag ataagtcaac
     1081 gccagtgctt taacttctgt cgtggcatca cccgcgacga aatcaataat ttgttcgcca
     1141 aaatgatgaa ttagcgttgc caacagtacg gcaaacaacg tcatgatcac caatgactgc
     1201 cgcgtcgcca ccctcgctcg tcgtcgatcc cgcttaccga gactaaatgc tacaacgaca
     1261 gtagtaccaa gatcgatagc tgcaaaaaaa gccataatga ccatattgaa gctgtccgcc
     1321 aaccccacgc cggccatcgc atcttttccc agccaactga ccagaaaagt gctcaggacc
     1381 cccatcaaca ggacacaggc gttctccatg aagataggaa cagcaagcgg ggttatctcg
     1441 cgctagaaca acactttgta gctcttgcgt ttagagtgcc agcgagtgcc gtgaacaacc
     1501 tggcgtaaag cagaggagat attcaaagcc gaccttaatt gcagaaagtg aaaccacatt
     1561 tcaaatgatg agggagaatc agcaaagctg caaagatttt cgccaacaaa ttgtctgcaa
     1621 atgcaacaaa ctgttgatag aaacggcaaa cagttgggaa atttaaaaat cgggtttgac
     1681 aaaatatttt tcgccgttaa gatgtgcctc aaca
//
LOCUS       DAJTRC010000251         1714 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.261, whole genome shotgun sequence.
ACCESSION   DAJTRC010000251 DAJTRC010000000
VERSION     DAJTRC010000251.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1714)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1714)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1714
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.261"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            1..1714
                     /gene="sat"
                     /locus_tag="OO357_004188"
                     /pseudo
     CDS             1..1714
                     /gene="sat"
                     /locus_tag="OO357_004188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001034089.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="serine protease autotransporter toxin Sat"
BASE COUNT          650 a          283 c          330 g          451 t
ORIGIN      
        1 tacggcatga tgatttataa aaatgataaa acatttcgta atcttgaaat attcggagac
       61 agcggctctg gagcatactt atatgataac aaactagaaa aatgggtatt agtcggaaca
      121 acccatggta ttgccagcgt taatggtgac caactgacat ggataacaaa atacaatgat
      181 aaactggtta gtgagttaaa agatacctat agtcataaaa taaatctgaa tggcaataat
      241 gtaaccatta aaaacacaga tataacatta caccaaaaca atgcagatac cactggtact
      301 caagaaaaaa taactaaaga caaagatatt gtgttcacaa atgggggaaa tgttctgttt
      361 aaggataatt tggattttgg ttgcggtggt attatctttg acgaaggcca tgaatataac
      421 ataaacggtc agagatttac atttaaagga gcaggaattg atatcggaaa agaaagcatt
      481 gtaaactgga atgcattgta ttccagtgat gatgttttac acaaaatagg ccccggtact
      541 ctgaatgttc aaaaaaaaca gggggcaaat ataaagatag gtgaaggaaa tgttattctt
      601 aatgaagaag gaacatttaa caatatatac cttgcaagcg gaaatggtaa ggtaatacta
      661 aataaagata attcccttgg caatgatcaa tatgcgggga tattttttac taaacgtggt
      721 ggcacgctag atttaaatgg acacaatcag acttttacta gaattgccgc cactgacgat
      781 ggaacaacaa taactaactc agatacaaag aaagaagccg ttctggcaat caataacgaa
      841 gactcctaca tatatcatgg gaacataaat ggcaatataa aactaacgca caatattaat
      901 tctcaggata agaaaactaa tgcaaaatta attctggatg gtagtgtcaa cacaaaaaat
      961 gatgttgaag tcagtaatgc cagtcttacc atgtaaggcc atgcaacaga gcatgcaata
     1021 ttcagaagca cagcgagtca ttgctccctg gtatttcttt gtggaacgga ctgggtcacc
     1081 gttttgaaag aaacagagag ttcatataat aaaaagttca attctgatta caaaagtaat
     1141 aatcagcaga cctcatttga tcagcctgac tggaaaaccg gggtgtttaa atttgataca
     1201 ttacacctga acaatgctga cttttcaata tcacgcaatg ccaatgttga aggaaatata
     1261 tcagcaaata aatcagctat cacaatcggc gataaaaatg cttacattga taatcttgca
     1321 gggaaaaata ttactaataa tggttttgac ttcaaacaaa ctatcagtac taatctatcc
     1381 ataggagaaa ctaaatttac aggtggcatc actgcacata acagccaaat agccataggt
     1441 gatcaagctg tagttacact taatggtgca acctttctga ataatactcc tataagtata
     1501 gataaaggag caaaagttat agcacaaaat tccatgttca caacaaaagg tattgatatc
     1561 tccggtgaac tgactatgat gggaatccct gaacagaata gtaaaactgt aacgccgggt
     1621 ctccactacg ctgctgatgg attcaggctg agtggtggaa atgcaaattt cattgccaga
     1681 aatatggcat ctgtcaccgg aaatatttat gctg
//
LOCUS       DAJTRC010000252         1693 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.262, whole genome shotgun sequence.
ACCESSION   DAJTRC010000252 DAJTRC010000000
VERSION     DAJTRC010000252.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1693)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.262"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..1162)
                     /locus_tag="OO357_004189"
     CDS             complement(<1..1162)
                     /locus_tag="OO357_004189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HCR5888889.1"
                     /translation="MERFDAIIIGAGAAGMFCSALAGQVGRRVLLIDNGKILGRKILM
                     SGGGRCNFTNLYVEPGAYLSQNPHFCKSALARFTQWDFIDLVNKHGIAWHEKTLGQLF
                     CDDSAQQIVDMLVDECEKGNVTFRLRSEVLSVAKDETGFTLDLNGMTVGCEKLVIATG
                     GLSMPGLGASPFGYKIAEQFGLNVLPTRAGLVPFTLHKPLLEELQVLAGVAVPSVITA
                     ENGTVFRENLLFTHRGLSGPAVLQISSYWQPGEFVSINLLPDVDLETFLNEQRNAHPN
                     QSLKNTLAVHLPKRLVERLQQLGQIPDVSLKQLNVCDQQALISTLTDWRVQPNGTEGY
                     RTAEVTLGGVDTNELSSRTMEARKVPGLYFIGEVMDVTGWLGGYNFQWAWSSA"
     gene            1394..>1693
                     /locus_tag="OO357_004190"
     CDS             1394..>1693
                     /locus_tag="OO357_004190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HCR5888890.1"
                     /translation="MLHLFAGLDLHTGLLLLLALAFVLFYEAINGFHDTANAVATVIY
                     TRAMRSQLAVVMAAVFNFLGVLLGGLSVAYAIVHMLPTDLLLNMGSSHGLAMVFSM"
BASE COUNT          391 a          460 c          416 g          426 t
ORIGIN      
        1 atgcactcga ccacgcccac tggaagttat agccccccag ccagccggta acgtccatca
       61 cttcaccgat gaagtacagc ccaggcactt tgcgcgcttc catcgtccgt gaagaaagtt
      121 cgttggtgtc cacgccgccg agcgtcactt cggcagtgcg atagccttca gtgccgttgg
      181 gttgtacgcg ccagtcggtc aatgtgctaa tcagtgcctg ttggtcacac acgttgagct
      241 gttttagcga aacatccggg atttgcccga gttgctgtaa gcgttcaacc aaccgcttcg
      301 gtagatgaac cgccagtgtg tttttcaggc tttgattcgg atgtgcgtta cgctgctcat
      361 tcaggaaggt ttcgaggtcc acatccggta gcagattgat actgacaaat tcacccggtt
      421 gccagtagct tgaaatctgc aacaccgccg gtccagataa gccgcggtgg gtgaagagta
      481 agttctcacg gaaaacggtg ccgttttcag cggtaatcac ggaaggcacc gccacgcccg
      541 ccagcacctg taactcttcg agcaacggtt tatgcagagt gaatggcacc agacccgcgc
      601 gggtcggcag cacgttgagg ccaaattgtt cggcaatctt ataaccaaac ggcgatgcgc
      661 ccagccccgg cattgacagc ccaccggtcg cgatgaccag cttttcgcaa ccgacagtca
      721 tgccgttcag atcaagcgtg aagcctgttt catccttcgc cacactcagc acttcgctac
      781 gcaatctgaa ggttacattg cccttctcgc actcatccac cagcatgtcg acaatctgct
      841 gcgcggagtc atcgcagaag agttgcccta acgttttctc gtgccaggcg ataccgtgtt
      901 tattgaccag atcaatgaaa tcccactggg taaaacgtgc gagtgcagac ttacaaaaat
      961 gcggattctg gctcagataa gcgcctggtt cgacataaag gttggtaaag ttgcagcgcc
     1021 caccgccaga cataaggatt ttgcgcccta gtattttacc attatcgatc agcagaaccc
     1081 ggcgtcctac ctgacctgcc agcgcagaac agaacatacc cgccgcacca gcgcctataa
     1141 taatggcatc aaacctttcc acgttgcgct cctcttagaa aaaacgggcg tgaattgtaa
     1201 agattcctca gtggtcgcac cagcatcaat attactaaaa ggaagtattt gcctgaatta
     1261 tataagataa ttattttttg agtgaaatcc atacaggagg caaatcaaaa aaagtctata
     1321 tttcactttg cccgcgccgc gaaagtcact gataatgcgc cgcgttcatg tcctcaaaat
     1381 ggcgtaacgt cctatgctac atttgtttgc tggcctggat ttgcataccg ggctgttatt
     1441 attgcttgca ctggcttttg tgctgttcta cgaagccatc aatggtttcc atgacacagc
     1501 caacgccgtg gcaaccgtta tctatacccg cgcgatgcgt tctcagctcg ccgtggttat
     1561 ggcggcggtg ttcaactttt tgggtgtttt gctgggtggt ctgagtgttg cctatgccat
     1621 tgtgcatatg ctgccgacgg atctgctgct taatatggga tcgtctcatg gccttgccat
     1681 ggtgttctct atg
//
LOCUS       DAJTRC010000253         1667 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.263, whole genome shotgun sequence.
ACCESSION   DAJTRC010000253 DAJTRC010000000
VERSION     DAJTRC010000253.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1667)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1667)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1667
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.263"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            5..751
                     /locus_tag="OO357_004191"
                     /pseudo
     CDS             5..751
                     /locus_tag="OO357_004191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            766..1188
                     /locus_tag="OO357_004192"
     CDS             766..1188
                     /locus_tag="OO357_004192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF977 family protein"
                     /protein_id="HCR5888891.1"
                     /translation="MAKPFTPEQREELKTRIVELVHQDGRVTIRQLSDETGISRASVG
                     RLCIELVASGDVYNSGYGLFPSEQARKDWQSARKKLSRVKVRKPVVVDPDLIWSLPDR
                     EIRRYDRRLNIICRECRKSEAMQRVLAFYQGNFQEAIL"
     gene            1246..1602
                     /locus_tag="OO357_004193"
     CDS             1246..1602
                     /locus_tag="OO357_004193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001514295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888892.1"
                     /translation="MEIKPEDELSNIVLFPIKEDDPRNQVNFLYKPSERPYCHHASVR
                     VDEKERQVRCKICGAVVEPFDWMLSVAKRETRLADDVRLLRQEEQERRRNIEKLIQIE
                     RNAKARIRRATKSRTE"
BASE COUNT          460 a          358 c          480 g          369 t
ORIGIN      
        1 gaccatgaaa aatattgcga caggcggcgt tctggaacgc atccgccgac tgaccccgca
       61 acatgtaacc gccccattca gaacggtagc ggagtggcgc gagtggcaac ttgctgaagg
      121 ccagaaacgt agcgaggaga tcaaccgcct gaatcgccag ttgcgggtgg aaaaaattct
      181 gaatcgctca ggcatccagc cgttgcaccg taaatgctcg tttgcgaatt accaggtgca
      241 gaacgacggc cagcgatacg cgttaagcca ggcgaaatcc atcgccgatg aactgatgac
      301 cgggtgtaca aattttgcgt tcagcggaaa acctgatacc gggtagaatc acttagcggc
      361 agctatcggg aatcgcctgc tgaaagacgg tcagacagtg attgtggtta ccgtggctga
      421 tgttatgagc gccctgcacg ccagctatga cgacgggcag tcaggcgaaa aatttttgcg
      481 ggagctgtgc gaagtggatc tgctggttct tgatgaaatt ggcattcagc gcgagacgaa
      541 aaacgagcag gtggtgctgc accagattgt tgatcgccgg acagcgtcga tgcgcagcgt
      601 ggggatgctg acaaacctga actatgaggt gatgaaaaca ttgctcggcg agcgggtgat
      661 ggatcgcatg gtcatgaacg gcgggcgctg ggttaatttt aactgggaga gctggcgtcc
      721 gaatgttagc cattcgaggg ttgttaagta gtttcaggag gatttatggc gaaacctttt
      781 actcccgaac agcgggaaga actgaagacg cgaattgtgg aactcgtgca tcaggacggt
      841 cgggtcacga ttcggcagtt gtccgatgaa acaggtatca gtcgtgcgtc tgtcggtcgc
      901 ttatgcatag aactggtcgc aagtggtgat gtatataatt ctggctacgg cttattcccg
      961 tctgaacagg ctcgcaagga ctggcaaagc gcccgcaaaa aactctcgag agtaaaggtg
     1021 aggaaaccgg ttgttgttga tccggacctt atctggtcat tacctgacag agaaatacgc
     1081 cgctacgaca ggcgcctgaa tataatctgt cgcgagtgcc ggaagagcga agctatgcag
     1141 cgtgtactgg ctttctatca gggtaatttt caggaggcga tactgtgaat gaaattagct
     1201 atcaggcttc aattaccgct ggcattcgca tcaaaggaga ggagcatgga aataaaacca
     1261 gaagatgagt taagcaatat cgttttattt ccgataaaag aggatgaccc tcgtaatcag
     1321 gttaattttc tttataagcc atcggaaaga ccatattgcc atcacgcctc tgtacgggtt
     1381 gacgaaaaag agcgtcaggt ccgctgtaaa atctgcggtg cggttgtgga gccgtttgac
     1441 tggatgctct ctgtggcaaa aagagaaacc agactggcag atgatgtaag gctattgcgc
     1501 caggaggaac aggaaaggcg gagaaatata gaaaagctga tacagattga gcgtaacgcg
     1561 aaagcgcgga tacgcagggc gacaaaatcc agaactgaat aattaaattt agcactgtta
     1621 aaaatttaat ccttaaccgg agggatttct gcaccctcaa atcatca
//
LOCUS       DAJTRC010000254         1627 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.264, whole genome shotgun sequence.
ACCESSION   DAJTRC010000254 DAJTRC010000000
VERSION     DAJTRC010000254.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1627)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1627)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1627
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.264"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO357_004194"
     CDS             complement(<1..767)
                     /gene="ipaH7.8"
                     /locus_tag="OO357_004194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH7.8"
                     /protein_id="HCR5888893.1"
                     /translation="MFSVNNTHSSVSCSPSINSNSTSNEHYLRILTEWEKNSSPGEER
                     GIAFNRLSQCFQNQEAVLNLSDLNLTSLPELPKHISALIVENNKLTSLPKLPAFLKEL
                     NADNNRLSVIPELPESLTTLSVRSNQLENLPVLPNHLTSLFVENNRLYNLPALPEKLK
                     FLHVYYNRLTTLPDLPDKLEILCAQRNNLVTFPQFSDRNNIRQKEYYFHFNQITTLPE
                     SFSQLDSSYRINISGNPLSTRVLQSLQRLTSSPDYHGP"
     gene            1096..1275
                     /locus_tag="OO357_004195"
                     /pseudo
     CDS             1096..1275
                     /locus_tag="OO357_004195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(1292..1627)
                     /locus_tag="OO357_004196"
                     /pseudo
     CDS             complement(1292..1627)
                     /locus_tag="OO357_004196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          487 a          277 c          360 g          503 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gcgagtcgac
       61 aatggattcc ctgaaatatt aatcctgtaa cttgaatcta attgtgaaaa actctccgga
      121 agagtggtta tctgattaaa atgaaaataa tattcctttt gtctgatatt gtttctatca
      181 gaaaattgag gaaaagtaac cagattattg cgctgagcac agagaatttc cagtttatcc
      241 ggtaagtcgg gtaatgttgt cagcctgtta taataaacat gtaaaaattt caatttttcg
      301 ggaagagccg gtaagttata tagcctgtta ttttcaacaa ataatgatgt taaatggttt
      361 ggcaaaacag gaaggttttc cagttgatta gaacgaacac ttaaagttgt taatgactca
      421 ggaagttctg gtatcacaga aagcctgtta ttatcagcat taagttcttt aagaaatgca
      481 ggcagctttg gcaatgatgt taatttatta ttttctacaa tcaaagcaga aatatgcttt
      541 ggtaattcgg gaagagacgt caaatttagg tctgataaat ttaatactgc ttcttgattc
      601 tgaaagcact gggagagtct gttaaaagca atgcctcgct cttccccggg agaagagttc
      661 ttttcccatt cagtcaggat tctcagataa tgttcattac tggttgagtt tgagttaata
      721 gagggggagc aagaaactga tgagtgtgta ttatttacag agaacataat gctaggtcaa
      781 ccttatattt gtgagaataa atatttaaaa ttgtatattt atacaaattt cacttaccat
      841 ttccgatatt aagttttaat agtttccatt ttacacataa cagaagttag ttgtaattta
      901 tttatggagg ttttctatgg aattcttttt ttattgtggc tgcagtcaat aataacactg
      961 aattcaacat tccagtgagc aaggaaatgt aagccgagta agtatatttg ttttacatca
     1021 tattttagtc aagctcattt attcctatta tagaatgaca aattaaaatt actgtaaaaa
     1081 aacgatacac atttaatacg agggaggccg gcagaaaaac ggcttgcggt caggcaggag
     1141 aaatgtgtgc cgcgaatgaa gtccttgtat gactggataa agctccagag gaaaactttg
     1201 tcaaaccatg ctgaaatggt aaaggcgttc aactatatcc tgaatcactg gaatgcactg
     1261 aatgagtttt gccgggtagt acttccaatc attagaatat gtgcttttca atgaaggaac
     1321 cgatgacttt ttcgtggatt tccacggaac gcgagaagca gattgtttta cgtgccagcc
     1381 gcttgatgcg ggggcggagg gtcaggttgt tacgttcata cgttgcgtga agattttgcc
     1441 ggtcaggtgt ttctcctccg gcacttctct ggcgtagctg ccccagtcat cgctggtcac
     1501 catgccgata cagaacggag tcaacagggc taacagttcc cgacaggtct catcattccg
     1561 tggcccgcaa gtgtaagcca acacaccgcc agttttagtg ttgtacgcat accagggcca
     1621 gtgttgt
//
LOCUS       DAJTRC010000255         1604 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.265, whole genome shotgun sequence.
ACCESSION   DAJTRC010000255 DAJTRC010000000
VERSION     DAJTRC010000255.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1604)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1604)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1604
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.265"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            240..509
                     /locus_tag="OO357_004197"
                     /pseudo
     CDS             240..509
                     /locus_tag="OO357_004197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000079945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            506..732
                     /locus_tag="OO357_004198"
                     /pseudo
     CDS             506..732
                     /locus_tag="OO357_004198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001585073.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            761..1351
                     /gene="ospG"
                     /locus_tag="OO357_004199"
     CDS             761..1351
                     /gene="ospG"
                     /locus_tag="OO357_004199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector protein
                     kinase OspG"
                     /protein_id="HCR5888894.1"
                     /translation="MKITSTIIQTPFPFENNNSHAGIVTEPILGKLIGQGSTAEIFED
                     VNDSSALYKKYDLIGNQYNEILEMAWQESELFNAFYGDEASVVIQYGGDVYLRMLRVP
                     GTPLSDIDTADIPDNIESLYLQLICKLNELSIIHYDLNTGNMLYDKESESLFPIDFRN
                     IYAEYYAATKKDKEIIDRRLQMRTNDFYSLLNRKYL"
BASE COUNT          477 a          330 c          377 g          420 t
ORIGIN      
        1 ctgaaaacgg ctcacaactg caccattcat aatcacatcg cataaaactg tcagcgccag
       61 tgataggaca ggtgaaacct gtcaatacgt accggcttac gccgggcttc ggcggtttta
      121 ctcctctgcc atatgcaaca acggagtgcc gccttacatg ccgctggcgc ggcatatcct
      181 ggtgacgata tctgaacagt tatatactgt gtatatacgt ggtaatgacg aggtggcaaa
      241 tggcacaggt taatatgagt gtaagaatcg acgcagaact gaaggatgct tttatggccg
      301 ctgcaaaaag catggaccgt aatggctctc agttaatccg ggattttatg cgccagaccg
      361 ttgaacggca gcacaattct tggttccgtg actaggttgc ggcaggccgt cagcaactcg
      421 aatgcggtga tgtgcttccc catgacatgg tcgaatcctc tgctgctgca tggcgggatg
      481 aaatgagcag gaagattgct gataaatgat ggagatgttc tggaccatgc tggccatcca
      541 ggacaggaag cgcattcgta gtacattgcg gagcagaacc tgatggccgc cattgaaccg
      601 gacgaacgga ttggttattc agccagcagc ctggcagggc agccgtataa aggccgcaac
      661 ggtcgtgtgg aaggaacatc tggaccacac aaggtagcgt gtaacgttat tttgtgtgaa
      721 aatctcttgt gatttttaaa tatcgtaaca aaggtatgac atgaaaataa catctaccat
      781 tattcaaaca ccttttccat ttgagaataa taattctcat gctggcatag taacggagcc
      841 cattctcggt aagttaatag gtcaggggtc gacagcagaa atctttgaag atgtgaatga
      901 ttcatctgct ttgtataaaa agtatgatct tattggcaac cagtacaatg agattctgga
      961 aatggcttgg caagaatctg agctttttaa tgctttttat ggcgatgaag catccgttgt
     1021 tatacagtat ggcggagatg tgtacctccg aatgctgcgc gtgcctggga ctccccttag
     1081 tgacattgat acagctgata tccctgataa tatagagagc ctttatctac agttgatatg
     1141 taaattgaat gagttgagta taatccatta cgatcttaat acaggtaata tgctgtatga
     1201 taaagaaagt gaaagtttat tcccaataga ttttcgcaat atttatgctg aatattacgc
     1261 tgcaaccaaa aaagataaag agattatcga ccgacgatta caaatgcgta caaatgattt
     1321 ttattcgtta ttaaacagga aatatttata gacgtatttg ttgatgctat aagccagagg
     1381 ctcttctatg agaatatggg gaccattccc tcgcgatatt aacgtaatat cttaattgta
     1441 tgcaccgcca gaatgcgttc agtgcgatca cggcagcaga caggtaaaaa tagcaacaaa
     1501 ccacccgaaa aaccgccgcg atcgcgtatg ataaatttta accacatgca tacctgtgca
     1561 acaaggcgaa tcacgcacgg atttccagcc agagacacct gaaa
//
LOCUS       DAJTRC010000256         1580 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.266, whole genome shotgun sequence.
ACCESSION   DAJTRC010000256 DAJTRC010000000
VERSION     DAJTRC010000256.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1580)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1580)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1580
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.266"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            399..1580
                     /locus_tag="OO357_004200"
                     /pseudo
     CDS             399..1580
                     /locus_tag="OO357_004200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000051932.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
BASE COUNT          415 a          297 c          349 g          519 t
ORIGIN      
        1 gtaacgacga ttactcctgt ctctgatgcc ggtggactac aagattttat ttactggcgt
       61 cctgatgcgg acagggctgg tatggaacct gtttatgtga tacttaatga tcctctggat
      121 tccgggcgat ttacgcgtaa acaactaaat aaaaatatct taaacatgca agtaattttg
      181 gtattctcga tacaagaata aatagtagta actactatag ctgcaaataa tgttaaaaac
      241 atttctatga tcatctttac atttttcatg attgacaatt gtttttggtt aaaaaaagac
      301 aactaatgag tgtgataatg caatactttg agtgattata taatgataag taaacaggtt
      361 tgcgacaatt ggttatcagt attacagaag gattaaatat gtcattattc acagaaccta
      421 aggaaattga aagattaccc tctgaagaga ttgaacgatt atatcccgtc ctgcgataca
      481 gggtctttat cagcattttc ctcggatata tggggtatta ttttgtccga aacaccacca
      541 gtgtgttatc cggtgtgctg cacatgtctg caaccgagat cggcattatc tcctgtgccg
      601 gttttttatc ctatggtata tcaaagtttg tttccggact tatttctgac agaagtaatt
      661 caaaagtatt tttgtctctt ggtttattcc tgtccggact ggtaaatttt ctgataggat
      721 atattcccgg gatcatcaca tccgttacgt tattttcaac aatgtattta cttaacggct
      781 ggatccaagg tatgggatat cctcctggtg ccaaaacact ggtgttctgg tatgaacatc
      841 gggaaaggat cacctgggcc acactgtgga atttatcgca caatgtcggg ggagctctgg
      901 cccccgttct gattggtttc agttttggtt ttttcggcga cagtgctctg gatcatgccc
      961 gggccgcatt tatcttccca ggagtactgt gtatggcaat gagtgtgctg atttacttta
     1021 ttcaggtgga cagacctgta tcagtggggc ttcctcccat tgaagaatgg aaaggtaacg
     1081 tcgtttcaca cccggctaaa ggccgtgaac aaggtccgag actctccatt cctgatatca
     1141 tcagaaaaca tatcatccga aataataaac tcatttactg ctgtatctat ggttcgtttg
     1201 tttatattct gagatacggt attgtttcct gggctcctaa gtttttaagt gacagtctgg
     1261 atgtcggtgg taaggatatg ggaaaactgg catcaatggg gggggttctg tttttgaaat
     1321 cggtggtgtg gctggtatgc ttcttgccgg atacctgtct gtcagactgt tcagaaactc
     1381 aaaaccatta acaaatactt tatttctggc acttaccatt attttattaa tcgcgtactg
     1441 gtacgtgcca tcaggcaatg aatatctctg gctaaactat acaatcctta ttctgttggg
     1501 gctggcagtg tatggtccgg tgatgtttat tggtctttat tcaatggagc ttgttcctaa
     1561 agaagctgcc ggagccgcat
//
LOCUS       DAJTRC010000257         1544 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.267, whole genome shotgun sequence.
ACCESSION   DAJTRC010000257 DAJTRC010000000
VERSION     DAJTRC010000257.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1544)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1544)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1544
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.267"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            614..1213
                     /locus_tag="OO357_004201"
     CDS             614..1213
                     /locus_tag="OO357_004201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inovirus-type Gp2 protein"
                     /protein_id="HCR5888895.1"
                     /translation="MQTYNTNPAYEMAPQLLEHFNQHLDSLFGVYSKLLPFRMDFAYR
                     KNTLSYRCACRYAMCAEMLQLINEVGEKLVGYAWVMEYTERKGLHIHFVGYLNGQSHR
                     SSYLVSRLMGDIWRRVTEGNGYYHWCRFNKNYPVNINHVIHYSDHKAVNDLRYAISYL
                     AKREQKECGIILKCSGLPEKSNRGRPRLDSPLPGICALV"
BASE COUNT          407 a          368 c          351 g          418 t
ORIGIN      
        1 cgtgttaata tcctggataa ctgtgtttga aggatttctg ttaataatct gctcctgcaa
       61 ctcacctgat gagtattagt ggtaatacat aatcttactc gtaaaagagt ataccttatg
      121 tcattaaaca gtcttccggc gtgttgttca atcctgtacc tctgttactt atctggtacg
      181 ttgatatgtg taatgctacg ttaattgcaa tatctggtta tggctttata ccttcagcat
      241 ttaaaatatt ttattaccgg agaaacaggg aagtggaaag actttcggga taatgtcagg
      301 ccattctgac gttaatcaca gtaccaaata acgagagggt aatggaagtt aaaacctgcg
      361 taagttcctc tgttgttcac tggaaaaagt gattgcccac acgcgttata aaggcctttc
      421 cgctgcagcc tggttaacag agcttcaggc gttatatgcg ctgtatggct gttacgtatg
      481 ctgttctttg cctggttctg atacccgtgg aagtcgttac cggaggaaac acgttcaccc
      541 tactacctga gaacatcttc ttcaaaaatt acggcaacgc ttcttcctgt gttacagaac
      601 acgaggaaga aacatgcaaa cttataacac caatcccgct tacgaaatgg ctccccagct
      661 cctggagcac tttaaccagc atctggattc attattcggg gtctattcga aactgttacc
      721 gttcagaatg gattttgctt accgaaaaaa caccctcagc tacagatgtg cctgcaggta
      781 cgccatgtgc gcggagatgc tgcaactgat taacgaggtg ggagagaaac tggtcgggta
      841 tgcctgggtg atggaatata cagaacgtaa agggcttcac attcattttg tgggctatct
      901 gaacggccag agccacagga gttcgtatct ggtatccaga ttgatgggag atatctggag
      961 gcgggttacc gagggtaatg gttactacca ctggtgccgg tttaataaaa attatccggt
     1021 aaacattaac cacgtcattc actattcaga tcacaaagcg gtgaatgatt tacgctatgc
     1081 catcagctac ctggccaaac gggaacagaa agaatgtggc atcatcctga aatgcagcgg
     1141 gcttcctgaa aaaagtaacc ggggtcgacc acgactggac agccccctgc cggggatatg
     1201 tgctctggtg taaacccctg aacgacaagt acgggctggc gctaatcttt gctgtcggtt
     1261 ccggcggcca cttcagcggc actgcgcatt ttttacggcg tggtcattcc gtatgaacca
     1321 tacggagatt taaccatgac acattcttta ccattccttc cggcaaccga tacagcacat
     1381 cccccaatct ccatctctgc ttactgactg ttttttacgt cagtgggcca ttgtgtgaca
     1441 accacagtgc tgtaacagca accagcctgc tttacccaaa ctccagtcca gattttaact
     1501 gaaccgccat tcacaccctg accacgctgc ccgtaccggg acgg
//
LOCUS       DAJTRC010000258         1461 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.268, whole genome shotgun sequence.
ACCESSION   DAJTRC010000258 DAJTRC010000000
VERSION     DAJTRC010000258.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1461)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1461)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1461
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.268"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..311
                     /locus_tag="OO357_004202"
     CDS             <1..311
                     /locus_tag="OO357_004202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418167.1"
                     /note="Involved in anaerobic NO protection; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator"
                     /protein_id="HCR5888896.1"
                     /translation="LDNVLQELGRERTIAMSLPEFEQSLFMAAQPDNLLLATAPRYCQ
                     YYNQLHQLPLVALPLPFDESQQKKLEVPFTLLWHKRNSHNPKIVWLRETIKNLYASMA
                     "
     gene            468..1217
                     /locus_tag="OO357_004203"
     CDS             468..1217
                     /locus_tag="OO357_004203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709485.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase superfamily
                     protein"
                     /protein_id="HCR5888897.1"
                     /translation="MATHFARGILTEGHLISVRLPSQCHQEARNIPPHRQSRFLASRG
                     LLAELMFMLYGIGELPEIVTLPKGKPVFSDKNLPSFSISYAGNMVGVALTTEGECGLD
                     MELQRATRGFHSPHAPDNHTFSSNESLWISKQNDPNEARAQLITLRRSVLKLTGDVLN
                     DDPRDLQLLPIAGRLKCAHVNHVEALCDAEDVLVWSVAVTPAIEKLSVWELDGKHSWK
                     SLPDIHSRANNPTSRMMRFAQLSTVKSFSPN"
     gene            1239..>1461
                     /gene="yieF"
                     /locus_tag="OO357_004204"
                     /gene_synonym="chrR"
     CDS             1239..>1461
                     /gene="yieF"
                     /locus_tag="OO357_004204"
                     /gene_synonym="chrR"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I chromate reductase YieF"
                     /protein_id="HCR5888898.1"
                     /translation="MSEKLQVVTLLGSLRKGSFNGMVARTLPKIAPASMEVNALPSIA
                     DIPLYDADVQQEEGFPATVEALAEQIRQAD"
BASE COUNT          375 a          373 c          360 g          353 t
ORIGIN      
        1 cactggacaa tgtgttacag gagctggggc gcgaacgcac gattgctatg agcctgccgg
       61 aattcgagca gtcactgttt atggcagcgc aacccgacaa tctgctactg gcgaccgcgc
      121 cgcgctactg tcagtactac aatcaactcc atcaactgcc gttggttgct cttcctctcc
      181 cgtttgacga aagccagcaa aaaaagctgg aagttccttt taccctgctg tggcataaac
      241 ggaacagcca taatccgaag atcgtctggt tacgggaaac cattaaaaac ctttacgcgt
      301 cgatggcata accgaatcgt atgaaacgcg acccaatttc acaataaaat gtaaaaaagt
      361 tgtaataagc ttgtctgaat cgaactttta gccgctttag tctgtccatc attccagtaa
      421 atgattactc ttgtattcat aatggaccat taagcatgga gcgaaaaatg gcgactcact
      481 ttgcccgagg gattttaacg gaaggacatc tgatttctgt tcgtctcccc tcccagtgtc
      541 atcaagaagc ccgaaacatt ccccctcatc gtcaaagccg ttttctggcg tccagaggtt
      601 tactcgcaga actgatgttc atgctgtatg gcattggcga attgccggaa atcgtcaccc
      661 tgccgaaagg taaaccggtt ttcagtgata aaaatttgcc ttcgttttct atttcctatg
      721 ccgggaatat ggttggcgtg gcgttaacaa ccgaaggtga atgtggcctc gatatggaac
      781 tacagcgtgc gacgcgcggg tttcatagcc cacacgcgcc cgataaccac accttttcca
      841 gcaatgaatc gctatggatc agtaaacaaa acgatcctaa cgaagcgcgg gcgcagctca
      901 ttacgctgcg ccgaagcgtg ctaaaactaa ccggtgatgt tttgaatgac gatccgcgcg
      961 atctgcagct gctgcccatt gccggacgcc tgaaatgtgc tcatgtaaat catgtagaag
     1021 cgttatgcga cgcggaggac gtgctggtgt ggtccgtggc ggtcacgccc gcgattgaaa
     1081 agctcagtgt ctgggagtta gatggcaaac acagctggaa aagcctgccg gatattcaca
     1141 gccgcgccaa caatcctacc agccggatga tgcgttttgc ccagctctct accgtgaagt
     1201 ctttttcgcc aaattgatag acaaccacag gagtcattat gtctgaaaaa ttgcaggtgg
     1261 ttacgttact ggggagcctg cgcaaaggct catttaatgg catggttgca cgtaccctgc
     1321 cgaaaattgc tccggcgagc atggaagtca atgcgttacc ttccattgcc gacattcccc
     1381 tgtatgacgc tgacgtacag caggaagaag gttttccagc aacggttgaa gctctggcgg
     1441 aacagatccg tcaggctgac g
//
LOCUS       DAJTRC010000259         1456 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.269, whole genome shotgun sequence.
ACCESSION   DAJTRC010000259 DAJTRC010000000
VERSION     DAJTRC010000259.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.269"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..839)
                     /gene="flhB"
                     /locus_tag="OO357_004205"
     CDS             complement(<1..839)
                     /gene="flhB"
                     /locus_tag="OO357_004205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar type III secretion system protein
                     FlhB"
                     /protein_id="HCR5888899.1"
                     /translation="MSDESDDKTEAPTPHRLEKAREEGQIPRSRELTSLLILLVGVSV
                     IWFGGVSLARRLSGMLSAGLHFDHSIIKDPNLILGQIILLIREAMLALLPLISGVVLV
                     ALISPVMLGGLVFSGKSLQPKFSKLNPLPGIKRIFSAQTSAELLKAILKTILVGSVTG
                     FFLWHHWPQMMRLMAESPITAMGNAMDLVGLCALLVVLGVIPMVGFDVFFQIFSHLKK
                     LRMSRQDIRDEFKQSEGDPHVKGRICQMQRAAARRRMMADVPKADVIVNNPTHYSVAL
                     QYD"
     gene            1075..>1456
                     /locus_tag="OO357_004206"
     CDS             1075..>1456
                     /locus_tag="OO357_004206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000455174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HCR5888900.1"
                     /translation="MEWQQLLNCNRRKDNLKKTNIAFLHPLASGRQEIERDYDRLLFA
                     APTRRLADKTQVFPLDQNDSIRTRLTHSHEVANFARGIGMRLAFEMKEHIFGEIPTGI
                     VVERDVPALLAAIGLAHDLGNPPFG"
BASE COUNT          388 a          378 c          351 g          339 t
ORIGIN      
        1 tcgtcatact gcaacgctac cgaatagtgg gtcgggttat tgacaatgac atccgctttc
       61 ggcacatcgg ccatcatccg acgccgtgcg gcagctcgct gcatctgaca gatccgtcct
      121 ttaacatggg ggtcgccttc gctttgtttg aactcatcac gaatatcctg ccgcgacatt
      181 cgcagctttt tcaggtggct gaagatttgg aaaaagacgt caaatcccac cattggaatg
      241 acaccaagca ccaccagcag tgcgcatagc cctaccaaat ccatcgcatt acccatggcg
      301 gtaatcggtg actcggccat taagcgcatc atctgcggcc agtgatgcca gagaaaaaac
      361 cccgtcacgc tgccaaccag gatggttttc aaaattgcct taagcaactc cgcgctagtc
      421 tgtgccgaga atatccgttt aatgcccggt agcgggttga gtttggaaaa cttcggctgc
      481 aaggatttgc cgctaaatac cagccccccc agcataaccg gagaaatgag cgccaccagc
      541 accacgccgc taatcagcgg tagcagcgcc agcatggctt ctctgatcag cagaataatt
      601 tgcccgagga tcagattcgg gtctttgata atactgtgat caaaatgcag cccagcggag
      661 agcatgcccg acaatcgacg ggccagcgac acaccgccaa accagataac actaacgccc
      721 accagcaaaa tcagcagtga ggtcagttca cgggaacgcg ggatttgccc ctcttcccgc
      781 gctttttcta gtcggtgagg tgtgggggct tctgttttgt cgtcgctctc gtcagacacg
      841 tcgccaatcc tggaaagagt attaagcgta aatgatgcca gagcgcaaag cgttcaatgg
      901 tttaagtaag gggcaaaaca ggcgggattt agggcgttta tgcaaaaata ggttcagttg
      961 cagaacgttt tgcgaaccat tttccagttc tgatatttag ccatttagcc tatagaatcg
     1021 cactccgcct ctctgttagt ttgctaataa ttaaattatt acaaaggtga tcaaatggaa
     1081 tggcaacaat tgcttaactg caaccgtcgg aaagataatc taaaaaaaac caacatagca
     1141 tttttacatc cccttgctag cggaagacaa gagatcgagc gggattacga ccgattactt
     1201 tttgctgcac caactcgtcg gcttgcggat aaaacacaag ttttcccgtt agatcaaaat
     1261 gacagcatac ggacgaggct gactcattct cacgaagtag caaactttgc tcgcgggata
     1321 ggaatgaggc tcgctttcga gatgaaggag cacatctttg gcgaaatacc cacggggatt
     1381 gtcgttgaac gcgacgttcc tgctctgtta gcagcgatcg gactggcaca cgatctcggt
     1441 aatccaccat ttgggc
//
LOCUS       DAJTRC010000260         1446 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.270, whole genome shotgun sequence.
ACCESSION   DAJTRC010000260 DAJTRC010000000
VERSION     DAJTRC010000260.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1446)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1446)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1446
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.270"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..758)
                     /locus_tag="OO357_004207"
     CDS             complement(<1..758)
                     /locus_tag="OO357_004207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5888901.1"
                     /translation="MLPTNNNHRLISNSFSTYSIDTSRAYENYLTHWTEWKNNRIQEE
                     QRDIAFQRLVSCLQNQETNLDLSELGLTTLPEIPPGIKSINISKNNLSLISPLPASLT
                     QLNVSYNRLIELPALPQGLKLLNASHNQLITLPTLPISLKELHVSNNQLCSLPVLPEL
                     LETLDVSCNGLAVLPPLPFSLQEISAIGNLLSELPPLPHNIHSIWAIDNMLTDIPYLP
                     ENLRNGYFDINQISHIPESILNLRNECSIDISDNP"
     gene            complement(1357..>1446)
                     /locus_tag="OO357_004208"
     CDS             complement(1357..>1446)
                     /locus_tag="OO357_004208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5888902.1"
                     /translation="RTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          383 a          233 c          347 g          483 t
ORIGIN      
        1 gggttatcac taatatctat tgaacattca ttcctcagat taagaatgct ttccgggata
       61 tgacttatct gatttatgtc aaaataaccg ttccttaaat tttccggcag gtatggaata
      121 tcggttaaca tattgtcgat tgcccatatg gagtgaatgt tgtgaggtag aggggggagt
      181 tcactaagaa gattccctat tgcgctaatc tcttgtaaag aaaatggtaa aggtggtaaa
      241 actgccagcc cattacatga tacatctaat gtttccagta gttctggtaa aacaggaaga
      301 gaacataatt gattatttga gacatgaagc tccttcaaag atatggggag tgtgggtagt
      361 gtgattagtt gattgtggga cgcattcaat aatttaagtc cttgaggcaa agcaggcagt
      421 tcaataagtc tgttatagct gacattaagt tgtgtaaggg acgcaggcaa tggggagatt
      481 aagcttaaat tatttttact tatattaatt gatttaattc ccggggggat ttcaggtaat
      541 gttgtcaggc ctaattcaga caagtccagg ttcgtctctt ggttttgtag acatgatact
      601 agtcgctgaa aagcgatgtc tcgttgttct tcttgtatgc ggttattttt ccattcagtc
      661 caatgggtta gataattttc atatgcgcgg ctagtgtcga ttgaataagt ggagaacgaa
      721 tttgaaatta atctgtgatt gttatttgtc gggagcataa atatcagggt gtattcttta
      781 tgttgagagg tgttggattc ttttttatct gctttgcaat gttattggtt cccttcagca
      841 gtagacagga acttctggag gaggcggata tatagtttgt gtaagcatat taactccata
      901 tgttatatat tgaagaactt ctgctttaca ctatcctaaa ctggatcggt ttaatgtaaa
      961 aaaacgatac tttttggggg ggggcaattt ttccttaagg tatgaaaaaa ggagctgaag
     1021 ctatataata gctattaatg ccactggtat cagtgaatca tgtatataaa aaactgtgtt
     1081 aaggaggctg tccattagcg gttttttatt cttctggaac cgcggcatct ttgcgtatct
     1141 ttttgtcttc tcaccgtctg gtcggtgttt tgctgagact gctaatttcc tgtctttgct
     1201 agtgctgtac tggtactgcc caatcatgat tggcgggggc gggcggagtt gagaccgcag
     1261 acacgtcgta tgcaagaacg tgctgcggct ggctggtgaa cttccgatag tgcgagtatt
     1321 gaatgatttc cagccgttac tgattttacg tgctaattag tgaacaaacc actcgtcagc
     1381 agactcccag gtatctttca gagtttcctg aacaaaagtt ttagctgaat ctttatcggc
     1441 ggtgcg
//
LOCUS       DAJTRC010000261         1413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.271, whole genome shotgun sequence.
ACCESSION   DAJTRC010000261 DAJTRC010000000
VERSION     DAJTRC010000261.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.271"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(1..264)
                     /locus_tag="OO357_004209"
                     /pseudo
     CDS             complement(1..264)
                     /locus_tag="OO357_004209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611134.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(379..1017)
                     /gene="ospI"
                     /locus_tag="OO357_004210"
     CDS             complement(379..1017)
                     /gene="ospI"
                     /locus_tag="OO357_004210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system effector OspI"
                     /protein_id="HCR5888903.1"
                     /translation="MINGVSLQGTAGYEAHTEEGNVNVKKLLESLNSKSLGDMDKDSE
                     LAATLQKMINPSGGDGNCSGCALHACMAMLGYGVREAPVPNEISEYMTGFFHRHLEQI
                     DSEGIVSHPNETYSKFRERIAENILQNTSKGSVVMISIEQATHWIAGFNDGEKIMFLD
                     VQTGKGFNLYDPVEKSPDAFVDENSSVQVIHVSDQEFDHYANSSSWKSKRLC"
     gene            complement(1170..1413)
                     /locus_tag="OO357_004211"
                     /pseudo
     CDS             complement(1170..1413)
                     /locus_tag="OO357_004211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558263.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          407 a          351 c          241 g          414 t
ORIGIN      
        1 cagcaagcgc tgtaactctt ttatttgttt tatagcggca gccagttctg aggcaggaac
       61 gacatcctct ccggcagcaa cagcagtgag acttccttcc agatattatt ttttccactt
      121 aagcaacagg ctgggctgaa taccatgcaa acgcgccaca tgggacaccg tcatgccagg
      181 ctccattgtc tgctgaataa tggcaatttt ttcctgagga gttttacgtt tacgaagctc
      241 cagccccagc aaagttccag tcatctcaaa attagaacta gtgttagaca tatatccaag
      301 cctatctctt attttaagat acatccactg tctggcgttt caaggggccg gctcaaatct
      361 atgtacaggc tctcagcatc agcaaagcct cttacttttc caactactac tgttagcata
      421 gtgatcaaat tcctgatcag atacatgaat tacctgaacc gaactatttt catctacaaa
      481 tgcatctggc gatttttcta caggatcata aagattaaat ccctttcctg tttgaacatc
      541 caaaaacata attttttcac catcgttaaa tccagctatc caatgagtgg cctgttcaat
      601 agaaatcatt actacacttc cttttgaagt attttgtaaa atattttctg caatacgctc
      661 acgaaattta ctataagttt cattaggatg tgacactatg ccttcactat caatttgctc
      721 taaatgccta tgaaaaaatc cggtcatata ctctgatatt tcattaggta cgggagcctc
      781 tcttactcca taccctaaca tagccataca agcatgaagc gcacaccctg aacagttacc
      841 atccccacca gacggattta tcattttctg taaagtcgct gccaattcag aatctttatc
      901 catatcacca agacttttag agtttaaaga ttccagcaat tttttaacat ttacatttcc
      961 ctcctctgta tgtgcctcat atccagcagt tccctgtaac gacaccccat taatcatatt
     1021 gtatcctgac agtatatata ctcactatat tttcttcata acaaatatcc ctctgatatg
     1081 acaacaaaaa acggagccgg attcctgttt ttgtgaactg cagctattcg tgtctaaaaa
     1141 gtgatccact taccaccaac ggcctaatat atacagaacg gagtaaacag gactaacagt
     1201 tcccgacagg tctcatcatt ccgtggcccg aaagtgtaag ccaacacacc gccagtttta
     1261 gtgttgtacg aataccagag ccagtgttgt tgcagccttg ctgccaacga agcgccactg
     1321 ctcatcaagt tcacagataa gggcaacatc agcgtgagcg actggcgcag atgttatccg
     1381 ccggggcggg agctttttaa tgtccgtatg acg
//
LOCUS       DAJTRC010000262         1404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.272, whole genome shotgun sequence.
ACCESSION   DAJTRC010000262 DAJTRC010000000
VERSION     DAJTRC010000262.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.272"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..167
                     /locus_tag="OO357_004212"
     CDS             <1..167
                     /locus_tag="OO357_004212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001285496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR5888904.1"
                     /translation="AIASTPHPSWRRICKTLIKNDFWCRTLSFSPNKPRHYERYLQRM
                     KERRKEWGTL"
     gene            152..790
                     /locus_tag="OO357_004213"
     CDS             152..790
                     /locus_tag="OO357_004213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HCR5888905.1"
                     /translation="MGDSVTPEILGNMIRQYFSQVRTEKESIQALNHLRRVLHEVSPF
                     AQEPVDCVLWVKADEVVANDYNPNVMALGEKKLLKQSLEKDGFTQPVVVSEEKNHYLV
                     VDGFHRQLLGREADAGKRLRGWLPVACINPERKGQAARIAATIRHNRARGKHQITLMS
                     DIVRDLSRLGWTNERIGTELGMDQDEVLRLKQISGLTELFQEEDFSSAWTVR"
     gene            1018..>1404
                     /locus_tag="OO357_004214"
     CDS             1018..>1404
                     /locus_tag="OO357_004214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888906.1"
                     /translation="MKKQIFINNKPPVVPYSGTHAKIFKYIEIPLPFFYFIYTSGEPF
                     HISVQNTVIYVSKYNGIFINKLVPFSLLFDRDISVLQRRDICVVRFTSEEISEHNVLF
                     DHDIERLKKISKAQLISPDYVLIDFSS"
BASE COUNT          407 a          257 c          347 g          393 t
ORIGIN      
        1 ccgctattgc cagtacacct cacccgtcct ggcgacgtat ctgtaagaca ctcataaaga
       61 atgatttctg gtgccggact ctctccttca gtccgaacaa accccggcac tatgaacgtt
      121 acctgcagcg tatgaaggaa aggaggaagg aatgggggac tctgtgacac cggagatact
      181 tggcaacatg attcgtcagt atttcagtca ggtacgaact gaaaaagaga gcatacaggc
      241 tctgaatcat ctgcgcagag tgcttcatga ggtcagtcct tttgcacagg agcctgtgga
      301 ctgtgtgttg tgggtgaaag cggatgaggt ggtggccaac gattacaacc ctaatgtcat
      361 ggcactaggt gagaaaaaac tactgaaaca gtctctggag aaagatggtt tcacacaacc
      421 ggtggtggtg tcagaggaga agaatcatta tctggtggtg gatggttttc atcgtcagtt
      481 actggggcgg gaagcagatg caggaaaacg cctgaggggc tggttgcctg tagcctgcat
      541 taatccggag agaaagggac aggccgcacg tattgccgcg accattcgac ataaccgggc
      601 cagggggaaa caccagatca ctttaatgtc tgatattgtc agggatcttt cacgtctggg
      661 ctggacgaat gaacgtattg gtacagagct gggaatggac caggatgaag tgctgaggct
      721 gaaacagatt agcgggctga cagagttgtt tcaggaagag gatttcagct cggcttggac
      781 ggttcgttga tgttgttaaa tatgggcggt atttctgaaa gttgatactt tgggtagaaa
      841 aaatgatacc gaaaactatg gaggatgtgc ttgtcaggtg actggacacc ataggtctta
      901 ggctttgata acgggataag atgagcggct tttctcatac catcaggtgt gatgatggcg
      961 cagatttaca aaatctgatt gattctaaag atgtataagt aaaaatcaag ctttgatatg
     1021 aaaaaacaga tatttattaa taataaacct cctgtagtcc cttactcagg gacgcacgcg
     1081 aaaatattta aatacattga aatccctctg ccgtttttct actttatcta tacatcaggc
     1141 gaaccattcc acatatctgt ccaaaatact gttatttatg tatcaaaata caatggtata
     1201 tttattaata aattagttcc tttttctctt ttatttgaca gggatatatc tgttcttcaa
     1261 agaagagata tttgtgttgt gcgatttacg tcagaggaaa taagtgaaca taacgtttta
     1321 tttgatcatg acattgaacg attaaaaaaa atttccaaag ctcagcttat atctcccgat
     1381 tatgttttga ttgatttttc tagt
//
LOCUS       DAJTRC010000263         1366 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.273, whole genome shotgun sequence.
ACCESSION   DAJTRC010000263 DAJTRC010000000
VERSION     DAJTRC010000263.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1366)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1366)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1366
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.273"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            400..1149
                     /locus_tag="OO357_004215"
     CDS             400..1149
                     /locus_tag="OO357_004215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HCR5888907.1"
                     /translation="MKRQLFTLSIVGVFSLNTFASFPPGNDVTTKPDLYYLTNDNAID
                     SLALLPPPPQIGSIAFLNDQAMYEKGRLLRNTERGKLAAEDANLSSGGVANVFSAAFG
                     SPITAKDSPELHKLLTNMIEDAGDLATRSAKEYYMRIRPFAFYGVSTCNTKEQDTLSR
                     NGSYPSGHTSIGWATALVLSEINPARQDTILKRGYELGDSRVICGYHWQSDVDAARIV
                     GSAIVATLHSNPVFQAQLQKAKDEFANNQKK"
BASE COUNT          387 a          278 c          275 g          426 t
ORIGIN      
        1 ccataaacag ggctttaccc gtaaatattg gctccttctg atgccattct atttcaggaa
       61 ggagtgtccg ttaaactcag gctacctcaa aaagttatcg gtgcgtttat cgaaaaacat
      121 atgttctact aattggagtc attgtctgta aaagcaactc aaaaatacaa gttgcttatt
      181 ccccttgttg gtaagaactt aattctaact gtttgttctg ggtgttttaa acgtcctcct
      241 ttttatgctt gttcataacg gatatttaat aggtaacctt tgttttagca tcttctgatt
      301 tatttaaatt atgtgatagt acacacaatt ttattatcca gtactttatg ctcttaataa
      361 aataattata aatatgtatc ctctataagg agatgttata tgaaaagaca gctttttact
      421 cttagtattg tcggggtatt ttctttaaat acctttgcat cattccctcc gggaaatgat
      481 gtgacaacaa aacctgacct ttactacctg acaaacgata atgctattga cagtctggca
      541 ttattaccgc ctccaccaca aatcggaagt attgccttcc tgaatgacca ggccatgtat
      601 gaaaaggggc gtttattgcg aaatactgaa agagggaagc tggcagctga ggatgccaat
      661 ctcagtagtg gtggcgtggc aaatgtattt tctgctgctt ttggttctcc gataacggct
      721 aaagattcac cagaattgca taaattactg acaaatatga ttgaggatgc cggagacctg
      781 gcaacgcgtt cagcaaaaga atattatatg cgtattcgac cttttgcttt ttatggtgtt
      841 tctacgtgta atacaaaaga acaggataca ttatccagaa atggctctta tccatcaggt
      901 catacatcga ttggttgggc aacagcactt gtattgtccg aaataaatcc ggcacgccag
      961 gataccattc tgaaacgggg ttatgaactg ggggacagca gggttatttg cggttatcac
     1021 tggcaaagtg atgttgacgc agcacgtatt gttggctctg ctattgtagc aacactgcac
     1081 tcaaatcctg tgttccaggc acaattacaa aaagcgaaag atgaattcgc taacaatcag
     1141 aaaaaataat gcttttagcc agtacgcctt ataaccccga ccttcagggc taatgccagt
     1201 cagttaagca actgactggc tctttttcag ggctgtgggg tatttccagt gcctctcctt
     1261 taccactctc ggaaaggccc tttcccttct tgtcggtaat ttcactaggc tgtgtccctt
     1321 aattacgtaa gcgttgataa aagagtcgga tgcagcccag tttcac
//
LOCUS       DAJTRC010000264         1363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.274, whole genome shotgun sequence.
ACCESSION   DAJTRC010000264 DAJTRC010000000
VERSION     DAJTRC010000264.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.274"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..>1363)
                     /gene="treF"
                     /locus_tag="OO357_004216"
     CDS             complement(<1..>1363)
                     /gene="treF"
                     /locus_tag="OO357_004216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalase TreF"
                     /protein_id="HCR5888908.1"
                     /translation="PDSKTFPDCAPKMDPLDILIRYRKVRRHRDFDLRKFVENHFWLP
                     EVYSSEYVSDPQNSLKEHIDQLWPVLTREPQDHIPWSSLLALPQSYIVPGGRFSETYY
                     WDSYFTMLGLAESGREDLLKCMADNFAWMIENYGHIPNGNRTYYLSRSQPPVFALMVE
                     LFEEDGVRGARRYLDHLKMEYAFWMDGAESLIPNQAYRHVVRMPDGSLLNRYWDDRDT
                     PRDESWLEDVETAKHSGRPPNEVYRDLRAGAASGWDYSSRWLRDTGRLASIRTTQFIP
                     IDLNAFLFKLESAIANISALKGEKETEALFRQKASARRDAVNRYLWDDENGIYRDYDW
                     RREQLALFSAAAIVPLYVGMANHEQADRLANAVRSRLLTPGGILASEYETGEQWDKPN
                     GWAPLQWMAIQGFKMYGDDLLGDEIARSWLKTVNQFYLEQHKMIEKYHIADGVPREGG
                     GGEY"
BASE COUNT          305 a          378 c          369 g          311 t
ORIGIN      
        1 gatactcgcc accgccgcct tcgcggggaa caccatcggc aatatggtat ttctcgatca
       61 ttttgtgctg ttccagatag aactgattca ccgttttcag ccagctgcgc gcgatttcat
      121 cacccagaag gtcatcgcca tacattttaa atccctgaat tgccatccat tgtaacggtg
      181 cccagccgtt gggtttatcc cactgttcac cggtttcgta ctcgcttgcc aaaatcccgc
      241 caggtgtcag taaccggctg cgtacggcgt ttgccagacg atcggcctgt tcatggttcg
      301 ccatgccgac atagagcggc acaatggcgg cagcggaaaa cagcgccagt tgttcgcgtc
      361 gccagtcgta atcgcggtag atgccgtttt catcatccca gaggtaacgg tttaccgcat
      421 cgcgacgggc actggccttc tggcggaaca gcgcttctgt ctctttctcg cctttcagcg
      481 ccgagatgtt ggcgatggcg ctctccagtt taaacaggaa ggcattcaga tcgatgggga
      541 tgaactgggt ggtacgaatg ctcgccagac gaccagtatc acgcagccaa cgggaagagt
      601 aatcccaacc ggaggccgct cccgcgcgta aatcgcggta cacctcgttg ggcgggcgac
      661 cagaatgttt cgcggtttca acgtcctcaa gccaggattc gtcacgcggc gtgtcgcgat
      721 cgtcccagta acggttgagc agcgatccgt ccggcatccg cacaacatgg cgataggcct
      781 gattagggat caacgattct gcaccgtcca tccagaaggc atattccatt ttaaggtggt
      841 cgagatagcg gcgcgcaccg cgtacaccat cttcttcaaa caactccacc atcagcgcaa
      901 aaaccggtgg ttgcgagcgg ctcaaataat aggtgcggtt gccgttgggg atgtgaccgt
      961 agttttcgat catccaggcg aagttatcgg ccatgcattt cagcaaatct tcccgaccac
     1021 tttccgccag tcccagcatg gtgaaatagg aatcccaata gtaggtttcg ctaaaacggc
     1081 cgcccgggac aatatatgac tgcggtagcg ccagcagaga agaccacgga atgtgatcct
     1141 gtggttcgcg ggttagcacc ggccacagct ggtcgatatg ctctttcagg gaattttgcg
     1201 ggtccgatac atactcgctg gagtagacct ccggcagcca gaagtgattt tcaacaaact
     1261 tgcgcaagtc aaaatcacga tgacggcgca ctttacggta gcggattaaa atatccagcg
     1321 ggtccatttt gggtgcgcag tcgggaaagg ttttactgtc ggg
//
LOCUS       DAJTRC010000265         1356 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.275, whole genome shotgun sequence.
ACCESSION   DAJTRC010000265 DAJTRC010000000
VERSION     DAJTRC010000265.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1356)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1356)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1356
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.275"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(3..485)
                     /locus_tag="OO357_004217"
     CDS             complement(3..485)
                     /locus_tag="OO357_004217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5888909.1"
                     /translation="MEINVTAPALLTDEHILQPFDCGNEVLSNWLRGRAMKNQMLNAS
                     RTFVICLEDTLRIVGYYSLATGSVTHAELGRSLRHNMPNPVPVVLLGRLAVDVCTQGH
                     GFGKWLLSDAIHRVVNLADQVGIKAVMVHAIDDDARAFYERFGFVQSVVAPNTLFYKV
                     "
     gene            complement(476..778)
                     /locus_tag="OO357_004218"
     CDS             complement(476..778)
                     /locus_tag="OO357_004218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858193.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HCR5888910.1"
                     /translation="MEVFMSTAASVRKTPREHQINIRATDEERAVIDYAASLVNKNRT
                     DFIMELAYQEAKNIILDQRLFVLDNERYDSFITQLEAPVQNAEGRERLMAVKPEWK"
     gene            1050..1322
                     /locus_tag="OO357_004219"
     CDS             1050..1322
                     /locus_tag="OO357_004219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_145811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888911.1"
                     /translation="MSHNLEHQKVHTRMVKEVLKAVARANNHPYKSVFADFITGHPSC
                     TVCFWETFHKMYPDSPYEYVTFCHTCRRFDLYETEAEMKADDPKWW"
BASE COUNT          398 a          342 c          288 g          328 t
ORIGIN      
        1 actcagactt tataaaacaa ggtattaggt gccacaactg actgaacaaa accaaaacgc
       61 tcataaaatg ctctggcgtc atcatcaatt gcatgtacca taacagcctt aatgccaacc
      121 tgatcagcca gattgacaac ccggtgaatg gcatcactta gtagccattt accgaaacca
      181 tgcccctggg tacaaacatc aacagccaga cgccctaaca gaacaacagg gacaggattg
      241 ggcatgttat gccgcaaact gcgaccaagc tcggcatgag tgacggaacc agtagctaat
      301 gagtagtatc ccacaatacg taaagtatcc tccaagcaaa taacgaacgt gcgagaagca
      361 ttgagcatct ggtttttcat agcccgacca cgtaaccagt tacttagcac ctcatttcca
      421 cagtcaaatg gctgaagtat atgctcatcc gtcaacaatg ctggcgcggt gacatttatt
      481 tccattcagg ctttaccgcc atcagacgtt cacgcccttc tgcattctgc acaggagctt
      541 ccagttgcgt aataaacgaa tcataacgtt cgttatccag cacaaataaa cgttgatcca
      601 ggataatgtt tttagcttcc tggtacgcca gttccatgat gaaatcggta cggttcttat
      661 tgaccaagct cgcagcataa tcaataacgg ctcgttcctc atcagttgcc cggatgttaa
      721 tctgatgttc tcgcggtgtt ttacggacgc ttgcagctgt agacataaaa acctccattg
      781 gtaacaattt gctatgcaat taatgttatc gcaaatggta acagaacgct atacagaatc
      841 acttacggga aaagcgatcc cctgataaaa acaatggcag agacctgaac attgtgacga
      901 tgatctgtgt cggcgcacaa atcatgtctg attacggccg tttaatgtga ttttgataat
      961 gaaatgcctg aaccacggca gcctggcggc tgcaaatcgg ggtgtggcag aatatgagct
     1021 ttcagagtaa ccatccagaa tgagttccca tgtcacacaa cctggagcac cagaaagtcc
     1081 acaccagaat ggtgaaagag gttctgaaag ccgtcgccag agccaataat cacccttata
     1141 agtccgtatt tgcagacttt atcaccggtc atccctcctg cacggtctgt ttctgggaaa
     1201 ccttccacaa aatgtacccg gattcaccgt acgaatacgt cacgttctgc catacctgcc
     1261 gtcgttttga tttatacgaa acagaagcgg agatgaaggc ggatgacccg aagtggtggt
     1321 gattttcagc cccgaccgct gaaacgcctg ttcaca
//
LOCUS       DAJTRC010000266         1331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.276, whole genome shotgun sequence.
ACCESSION   DAJTRC010000266 DAJTRC010000000
VERSION     DAJTRC010000266.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.276"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            78..998
                     /locus_tag="OO357_004220"
     CDS             78..998
                     /locus_tag="OO357_004220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5888912.1"
                     /translation="MMNNKVSFTNSNNPTISLSAVIYFPPKFDETRQYPAIVVSHPGG
                     GVKEQTAGTYAEKLAEKGFVTIAYDASYQGESGGEPRQLENPYIRTEDVSAVIDYLTT
                     LSYVDNTRIGAMGICAGAGYTANAAIQDRRIKAIGTVSAVNIGSMFRNGWENNVKSID
                     ALPHIEAGSNARTSDISSEEYAIMPLAPMKEADAPNEELRQAWEYYHTPRAQYPTAPG
                     YATLRSLNQIITYDAYHMAEVYLTQPMQIVAGSQAGSKWMSDDLYDRASSQDKRYHIV
                     EGANHMDLYDGKAYVAEAISVLAPFFEETL"
     gene            998..1331
                     /locus_tag="OO357_004221"
                     /pseudo
     CDS             998..1331
                     /locus_tag="OO357_004221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000680621.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
BASE COUNT          373 a          305 c          325 g          328 t
ORIGIN      
        1 ctaatcaact taatgatcaa tatctatagt gatatcacca accgtcaaaa cgaaccgaca
       61 actccatgag gaactgaatg atgaataata aagtcagctt cactaacagc aataatccaa
      121 ccatctcttt gtctgcagtg atctatttcc ctccaaaatt tgatgaaacc cgtcagtatc
      181 cggcaattgt ggtatctcac ccaggtgggg gcgttaaaga gcaaacggcc ggaacctatg
      241 ccgaaaagct ggcggaaaag ggatttgtca ctattgctta tgacgcatct tatcaaggtg
      301 aaagtggcgg cgagccgcgt cagttagaaa acccatacat tcgtactgaa gacgtcagtg
      361 ccgtgattga ttacctgaca acgctttctt atgtcgacaa tacccgaatt ggtgcgatgg
      421 gaatttgtgc cggtgcagga tatacagcca atgctgcaat tcaggatcgc cgcatcaagg
      481 ccatcggtac cgttagtgcg gtcaatattg gttcgatgtt ccgcaatggt tgggaaaaca
      541 atgtgaaatc tattgatgct ctgccacaca ttgaagctgg ctcaaacgcc cgaaccagcg
      601 atattagcag cgaagaatac gccattatgc cactggctcc aatgaaggag gccgatgcgc
      661 cgaacgaaga actgcgtcag gcctgggagt attaccacac acctcgtgcg cagtatccaa
      721 cagcaccggg ttacgctact ttgcgcagcc ttaaccagat tattacctat gatgcttacc
      781 atatggcgga agtgtacctg actcagccga tgcagattgt ggcgggcagc caggcaggga
      841 gcaaatggat gagtgacgac ctgtacgatc gggcatcaag ccaggataaa cgctatcaca
      901 ttgttgaagg cgcaaaccat atggatcttt atgacggcaa agcctatgtg gctgaagcaa
      961 tttcagtatt agctccgttc tttgaggaaa ctctgtaatg aaacatattc aaatccgcaa
     1021 tagtgatatg gcctggcata ttgctgccaa tattcagttt ccaccaaatt ttgatgagtc
     1081 aaaacagtat ccggcgatta tcagcgtcca tccttttggc agttgtaagg agcagacgtc
     1141 tggcaatatc tatggcaagg cattggctga aaaaggctat gtcgttctcg cttatgacgc
     1201 cagtttccag ggagaatctg gtgggtagcc tcgttggata gaagatccga cacagcgcgt
     1261 agaggatatt agccgtgtta tcgattatgc agtaactctg ccatatgttg atgctgaacg
     1321 cattggcgtg c
//
LOCUS       DAJTRC010000267         1319 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.277, whole genome shotgun sequence.
ACCESSION   DAJTRC010000267 DAJTRC010000000
VERSION     DAJTRC010000267.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.277"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..430
                     /locus_tag="OO357_004222"
     CDS             <1..430
                     /locus_tag="OO357_004222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001544497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888913.1"
                     /translation="SPDTNAATVNQTLKCDSKARADQPGACPTTYELYEGDATYRAAI
                     DKALNPVGLNGMFGEDGYMDGPDGGAYPVNINGTTWVEGGGCKAHACGWDYIVTLYNP
                     KTHKVVGYYYNIDPGYLIWFGETGVHEFAYLVRDYVNKTN"
     gene            550..723
                     /locus_tag="OO357_004223"
     CDS             550..723
                     /locus_tag="OO357_004223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001278287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF905 family protein"
                     /protein_id="HCR5888914.1"
                     /translation="MSRLLPEVPFSREQAVVITTAYRNVFIDDDQGTHFRRVIRNAET
                     DAGRFASDGLLRQ"
     gene            823..>1319
                     /locus_tag="OO357_004224"
     CDS             823..>1319
                     /locus_tag="OO357_004224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF945 domain-containing protein"
                     /protein_id="HCR5888915.1"
                     /translation="MRLASRFGYAANQIRRDRPLTHEELMHYVPSIFGEDRHTSRSKR
                     YAYTPTITVLESLQREGFQPFFACQTRVRDPGRREYTKHMLRLRRAGQITGQHVPEII
                     LLNSHDGSSSYQMLPGYFRAICTNGLVCGQSLGELRVPHRGNVVEKVIEGAYEVVGVF
                     DRIEE"
BASE COUNT          343 a          321 c          329 g          326 t
ORIGIN      
        1 aagtcctgac acaaacgccg ccactgtaaa tcagacactt aaatgcgact caaaagcccg
       61 ggctgaccag cctggtgcat gtccaacaac gtacgagtta tacgaaggtg acgctaccta
      121 cagagctgct atcgacaaag cattaaaccc tgttgggtta aacggaatgt ttggggaaga
      181 cggctatatg gatggtcctg atggtggtgc gtatcctgta aacattaacg gaacgacctg
      241 ggtagaaggt ggcggttgta aagcccacgc ctgcggctgg gactatatcg taacactcta
      301 taacccaaaa acgcataaag tcgttggtta ctattacaac atcgaccctg gttacctgat
      361 ttggtttggt gaaactggcg ttcacgaatt cgcgtacctg gtcagggact acgtaaacaa
      421 aacaaattaa aggcgtaagt tatgcggttc atcttcgcgt tattaattta ttgatatcaa
      481 aagtaaatac ggctctgcca tgatgacagg gacttatatt ttcagggaac caaatacagg
      541 tctggttgta tgtcacgttt attgcccgaa gtccctttct cacgcgagca ggctgttgtc
      601 atcacaacag cttaccgcaa tgtgtttatc gacgatgacc agggaactca tttccgtcgg
      661 gttatccgca acgcagagac tgacgccgga aggttcgcca gtgacggtct tctcagacaa
      721 taaacctcat taatttcgtc catcaggccg cgtcttctcc gggagacgtg gctttttcat
      781 ttattacacc agtaaatctt aaccaccgat aaggagcaaa ttatgcgact agccagtcgt
      841 tttggttatg ctgcaaacca gatacgccgt gaccgtccgc tgacacacga agaactgatg
      901 cactatgttc ccagtatttt cggtgaagac cggcatactt cccgcagtaa acgctatgcg
      961 tacactccca ccatcaccgt actggaaagc ctgcagcggg aaggctttca gccattcttc
     1021 gcctgccaga cacgtgtgcg tgacccgggt cgccgggaat acacaaaaca catgctgcgt
     1081 ctgcggcggg ccggacagat aaccggtcag catgtccctg aaattattct gctcaactct
     1141 catgacggtt catccagcta ccagatgtta cccggatatt ttcgtgccat ttgtaccaat
     1201 ggactggtct gcggtcagtc tctgggagaa ttgcgtgttc cacaccgggg aaatgtggtg
     1261 gagaaagtta ttgaaggggc ttacgaggtg gtgggcgtgt ttgaccggat agaggagaa
//
LOCUS       DAJTRC010000268         1291 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.278, whole genome shotgun sequence.
ACCESSION   DAJTRC010000268 DAJTRC010000000
VERSION     DAJTRC010000268.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1291)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1291)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1291
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.278"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(1..412)
                     /locus_tag="OO357_004225"
                     /pseudo
     CDS             complement(1..412)
                     /locus_tag="OO357_004225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
     gene            complement(463..1038)
                     /locus_tag="OO357_004226"
                     /pseudo
     CDS             complement(463..1038)
                     /locus_tag="OO357_004226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001368704.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            1042..1291
                     /locus_tag="OO357_004227"
                     /pseudo
     CDS             1042..1291
                     /locus_tag="OO357_004227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000998048.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
BASE COUNT          288 a          370 c          323 g          310 t
ORIGIN      
        1 catgggccat acacgcagct tccgttattc tgccggattc gtataacgcc cggtaaccac
       61 cgtaagcatc tgcctgaagc acaccgctgt aaccggctag gtgattctgt ggatggatac
      121 ctttccggtc cgggctgtac gcgaaccaga ccgccggggg catctgtgaa ccggcgttac
      181 ggtcatcacg gacatagacc cacagccggg cagttcttgt tttaccactg cccggctcct
      241 ggaccggaac ggggatatca tccgcatgaa ctttaccggg catcagtaca tactggcgca
      301 ggacgtcata cagcggctcc agcagttcag caacggcacc ggtccagcga cccagtgtgg
      361 cacggctcag ctccacgccc tgacgacggt atatttctga ctggcggtat aactgtcaac
      421 gacggatgaa aagtggctct tccgcaacct gggtggagat tttcatgcca gtggactcat
      481 taccctctgc ctgagtagtt caaatcctgc tcgaccatac atctggcgtt tcagcatctt
      541 cttcaggcga tttacatgac cttcgacaac accattactc cagcgactac tgattgcctc
      601 acatatagct gcagcatcag cttccatccc cgccgccacg cgccgaaatt ctgctgaacc
      661 gctttcgtgg actcgagtga accaactgct tagctgtgat ttattttggg ttttcagaat
      721 acggtagaac tcgagtgcca gttgctgcgc tattttcagc tcaggttctt tttcacacat
      781 cagactaata aatcgggaag cataattttc ttcatccctg gttattctcc agggcatcaa
      841 ccagcggctt acccgggaga ctgaaggaag tctgacggca gtagtaaccg gtgggttcca
      901 gcctttacgc catttggcaa ccgcatccct gacgatggtt tcactgcctg taaatccccg
      961 ggccaccatt tcccgccata tccggctggc gttataatta ccactttctc gttgctcttt
     1021 taaccactcc ctccatggtg tcagcgaaaa acgaaaccgc tgttgaaatc cctggaaagc
     1081 tggttgcgga aaagatgaag acccagtcgc gacactcaga gttggcgaag gcgttcgcgt
     1141 acgcacttaa ccagtggccg gcactgacgt actatgcgaa cgatggctgg gtggaaatcg
     1201 acaacaacat cgctgaaaat gccttgcggg cggtcagtct gggtcgtaaa aacttcctgt
     1261 tcttcggctc tgaccatggt ggtgagcggg g
//
LOCUS       DAJTRC010000269         1282 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.279, whole genome shotgun sequence.
ACCESSION   DAJTRC010000269 DAJTRC010000000
VERSION     DAJTRC010000269.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1282
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.279"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..100
                     /locus_tag="OO357_004228"
     CDS             <1..100
                     /locus_tag="OO357_004228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016234696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5888916.1"
                     /translation="RWNDEKPETIQPQSRPSSAITVSKTGYVFFDR"
     gene            208..>1282
                     /locus_tag="OO357_004229"
     CDS             208..>1282
                     /locus_tag="OO357_004229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HCR5888917.1"
                     /translation="MRPSELSDLLWAQVDRVAPHLLPNGKIEGHEWVAGNVNGDKGNS
                     LKVNLIGKKKWADFAEGDGGDMLDLWMACRGINLHQAMQEAKAFLGIKDDDHHFDARR
                     EKKFSRPDRKKIARYVTRTESHLEYLQSRGISPEVVKRYEVVSGKVWNGERELDALVL
                     PYKRDGELLQVKRISTERPDGKKVIMAEGDCEPCLFGWQALDAGVRVVVLCEGEIDCM
                     SYAQYGISALSVPFGGGKGAKQQWIEFEYHNLDRFEEIFISMDVDDVGREAAREIVSR
                     LGEHRCRLVTLPYKDINECLMNGVTEDEIWQYIGTASYFDPEELYSAREFYQDTINAF
                     YGKQQYLFNPPWESLADKFQFREA"
BASE COUNT          341 a          281 c          362 g          298 t
ORIGIN      
        1 gcgctggaac gacgagaagc cagaaaccat tcaaccacaa tccagaccat catccgcaat
       61 caccgtatcg aaaactggct atgtgttttt cgacaggtga accatgaaat caaaaatcaa
      121 atcgctactg gtcgctggtt ataaccacgg ctggttaagt atttcgtttg tcgatttctg
      181 gtttaaaaat ctcaatctga gggaatcatg aggccaagtg aacttagcga cctgctttgg
      241 gcgcaggttg acagggtggc tccgcacctg ttgccaaacg gcaagataga ggggcatgag
      301 tgggttgccg gtaacgtcaa cggtgacaag ggaaacagcc ttaaggtcaa ccttatcggc
      361 aagaaaaaat gggctgattt cgctgaggga gacggcggtg acatgcttga tttgtggatg
      421 gcatgtcgtg gaattaacct gcatcaggct atgcaggaag cgaaagcatt tctcggtatc
      481 aaggatgacg atcaccattt cgatgccaga cgtgagaaga aattctccag acctgatcgt
      541 aagaaaatcg cccgctacgt taccagaaca gaatcccatc ttgagtacct gcaatcgcgt
      601 ggcatatcgc cagaagtcgt aaagcgctac gaggttgtca gcggcaaggt gtggaatgga
      661 gaacgagaac tggatgctct ggtgcttccg tacaaacgcg atggtgagtt gttgcaggtc
      721 aagcgaatca gcactgagcg cccggacggg aagaaagtca ttatggcaga aggtgattgc
      781 gaaccttgcc tgttcggatg gcaggctctg gacgctggcg tgagggtggt tgtgctttgc
      841 gaaggcgaaa ttgattgtat gagctatgcg caatacggca tctcggcgtt atccgtgccg
      901 tttggtggcg ggaaaggcgc taagcaacag tggattgagt ttgagtatca caacctcgac
      961 aggtttgagg aaatattcat ctcgatggac gttgatgatg ttggtcgtga agccgcaagg
     1021 gaaatcgtaa gccgactcgg tgaacatcgt tgccgtcttg ttactctgcc gtacaaagac
     1081 atcaacgaat gcctgatgaa cggtgttacc gaggatgaaa tctggcagta catcggcacg
     1141 gcatcctact tcgatcctga agaactctac agtgcgcgag agttttacca ggacactatc
     1201 aacgctttct acggcaagca gcagtatctg tttaatccac cgtgggaatc tctggcagat
     1261 aaattccagt tccgtgaggc ag
//
LOCUS       DAJTRC010000270         1277 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.280, whole genome shotgun sequence.
ACCESSION   DAJTRC010000270 DAJTRC010000000
VERSION     DAJTRC010000270.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1277)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1277)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1277
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.280"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO357_004230"
     CDS             complement(<1..833)
                     /gene="ipaH4.5"
                     /locus_tag="OO357_004230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="T3SS effector E3 ubiquitin-protein ligase
                     IpaH4.5"
                     /protein_id="HCR5888918.1"
                     /translation="MKPINNHSFFRSLCGLSCISRLSVEEQCTRDYHRIWDDWAREGT
                     TTENRIQAVRLLKICLDTREPVLNLSLLKLRSLPPLPLHIRELNISNNELISLPENSP
                     LLTELHVNGNNLNILPTLPSQLIKLNISFNRNLSCLPSLPPYLQSLSARFNSLETLPE
                     LPSTLTILRIEGNRLTVLPELPHRLQELFVSGNRLQELPEFPQSLKYLKVGENQLRRL
                     SRLPQELLALDVSNNLLTSLPENIITLPICTNVNISGNPLSTHVLQSLQRLTSSPDYH
                     GP"
BASE COUNT          358 a          229 c          286 g          404 t
ORIGIN      
        1 gggccgtggt agtccggcga agaggttaat ctttgcaggg attgcagaac gtgagtcgac
       61 aatggattcc ctgaaatgtt aacattcgta caaatgggca atgtgattat attttcgggt
      121 aatgaagtta gtaggttgtt ggaaacatcc agtgccaata gttcttgcgg taatctggat
      181 aatctgcgta gttgattttc acctaccttc aaatatttta agctctgagg aaattctggt
      241 agttcctgta gtctgttgcc ggaaacaaag agttcttgta gtctatgagg caattcaggc
      301 aagacagtaa ggcgattacc ttcaatacgt aatattgtta gcgttgatgg aagctctggt
      361 aacgtctcca gactattaaa acgtgccgag agtgattgta aatatggtgg taatgatggc
      421 agacatgaca aatttcgatt gaatgaaata ttaagcttaa tcagttgaga tggaagtgtc
      481 gggagtatat tcaagttgtt accatttaca tgaagttctg tcaaaagcgg agaattttca
      541 ggtagggaga ttaactcatt gttggaaata ttaagttcac gtatatgcaa agggagtggt
      601 ggtaaagaac gtagtttcag taagcttaaa ttgagaacag gctcccgggt atccagacat
      661 attttcaata atcgaaccgc ctggatgcga ttttctgttg ttgttccttc cctagcccag
      721 tcatcccaga tgcggtggta atctctggta cactgttctt ctaccgataa acgagatata
      781 catgataagc cacaaaggga acgaaaaaaa gaatgattgt tgatcggttt catttattaa
      841 cccctcacta ttcatatctt ggtctattaa tgcaaataaa acgagaaagt ctttaaaaaa
      901 acaatcctag agctttatcg gcgttttcat tccggctgga ggaatatgtt cctgtctttc
      961 cagtaaccgt cgattgttat atcagtctac ccatgttaat gtgaccagtt ctactttcat
     1021 ccagtttgga ttgatcttta tcccctatat tttcaaacaa tttttatcat ttaactcatt
     1081 actgtctcga tattcccata gcaaacgatc tggttggtta tcccctcaaa aaagtcagtt
     1141 gcacgccgta aatccctgcc ggtattgagc gaggatttac gggttgttgt taaggtccgt
     1201 aatcgggtca cagtttttcc ggagtcaatc cggtctgcgg tttatgctta tgcgacgtga
     1261 ttatgaatgg tgcagtc
//
LOCUS       DAJTRC010000271         1257 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.285, whole genome shotgun sequence.
ACCESSION   DAJTRC010000271 DAJTRC010000000
VERSION     DAJTRC010000271.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1257)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1257)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1257
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.285"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            24..383
                     /locus_tag="OO357_004231"
     CDS             24..383
                     /locus_tag="OO357_004231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RusA family crossover junction
                     endodeoxyribonuclease"
                     /protein_id="HCR5888919.1"
                     /translation="MRHEFILPYPPTVNTYWRRRGSTYFVSKAGERYRRDVALIVRQQ
                     RLKLSLSGRLAIKIIAEPPDKRRRDLDNILKAPLDALTHAGLLIDDEQFDETNIVRGL
                     PVPGGRLGIKITELECA"
     gene            380..1063
                     /locus_tag="OO357_004232"
     CDS             380..1063
                     /locus_tag="OO357_004232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707875.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5888920.1"
                     /translation="MNNQYLQFVREQLIIATADLSGATKGQLEAWQENAMFDTGRYRR
                     KKIRYRDEVTGKMITRDNPPIPGKQSLAKGASIPLVSQVAFSTSSWRRAVLSLEEHYK
                     AWLLWCYSGSICWEYQIAITQWAWNEFNAQSGTRKIAGKTQERLKKLIWLAAQAVKAE
                     LFGGEGYEYQELALLAGVTTKNWSKTFTRHWVAMKHIFHRLDSEALLFVMRTRSKKRR
                     HFQSKVLQK"
BASE COUNT          345 a          249 c          349 g          314 t
ORIGIN      
        1 ctggtgcagg aggtgctgcg ctgatgcgcc atgagtttat tttaccttat ccgccgacgg
       61 tgaatactta ctggcgacgt cgtggcagca catattttgt atcaaaagcc ggtgagcgtt
      121 atcgccggga tgtggcgctt attgtccgtc agcagcgact gaaattaagc ctgtccggaa
      181 gactggcgat aaagattatt gcagagccac cggataagcg gcgtcgtgac ctggacaata
      241 tcctgaaagc accactggat gcactgacac atgcggggtt gcttatcgac gacgagcagt
      301 ttgatgaaac caatattgtg cgcggactgc ctgttcctgg tggtcggttg gggataaaaa
      361 tcacagagct ggagtgtgca tgaataacca gtatttacag tttgttcgtg agcagctcat
      421 tatcgccacc gctgatttga gtggggcgac aaaaggtcag cttgaagcct ggcaggagaa
      481 tgccatgttc gatacagggc gttacaggcg taaaaaaatc cggtaccgcg atgaggtgac
      541 cggaaaaatg ataacgcggg ataatccacc aatcccggga aaacaatcac tggcgaaggg
      601 ggcgtcaatt cctctggtca gtcaggttgc gttttcgaca tcatcgtggc ggcgggctgt
      661 tctgtctctt gaagagcact ataaagcctg gttgttgtgg tgttacagcg ggagtatttg
      721 ttgggaatat cagatcgcga taacacagtg ggcgtggaat gaatttaatg ctcaatccgg
      781 taccagaaaa attgcaggga aaacgcagga acgcctgaaa aaattaatct ggctggcggc
      841 gcaggcagta aaagcagaac tttttggtgg ggaaggttat gaataccagg agctggcatt
      901 actggcggga gtgacaacta aaaactggtc caaaacattt actcgtcact gggttgcaat
      961 gaaacacatt tttcaccgac tggatagtga ggctttattg tttgtaatga gaacacgttc
     1021 aaaaaaaaga cggcattttc aaagcaaagt gttgcaaaag tagattgaaa ggcatatatt
     1081 catgcaaatc tgatactttg ccgattttgt acgtgatggc aaaagcaaac aaaacccgcc
     1141 cacaagcggg ttttttgtgc cacttatctc ggatagacat ggtgaatgcg ctggtggagg
     1201 agataagggt gatttttgaa tgcttgcaac attgatttcg taacgttatt atcctgc
//
LOCUS       DAJTRC010000272         1248 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.286, whole genome shotgun sequence.
ACCESSION   DAJTRC010000272 DAJTRC010000000
VERSION     DAJTRC010000272.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1248)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1248)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1248
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.286"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            387..671
                     /locus_tag="OO357_004233"
     CDS             387..671
                     /locus_tag="OO357_004233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HCR5888921.1"
                     /translation="MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQA
                     LSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQSWADSLSTDHLLPVPR"
     gene            671..946
                     /locus_tag="OO357_004234"
     CDS             671..946
                     /locus_tag="OO357_004234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HCR5888922.1"
                     /translation="MELKWTSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLT
                     HPRMGEQLFQFEPREVRRIFTGEYEIRYELTGQTIYVLRLWHTRENR"
     gene            complement(1172..>1248)
                     /locus_tag="OO357_004235"
     CDS             complement(1172..>1248)
                     /locus_tag="OO357_004235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS1 family transposase"
                     /protein_id="HCR5888923.1"
                     /translation="LSFSKSVELHDKVIGHYLNIKHYQ"
BASE COUNT          314 a          308 c          322 g          304 t
ORIGIN      
        1 gagtaatcgt cgttaccggt atttcgactg gtttgtactg gtgtgagcgg acaaaactac
       61 tgtacgtctc tcatacacgc tcgtaagcgg ccctcacggc ccgctaacgc ggagatacgc
      121 cccgactgcg ggtaaaccct tgtcgggacc actccgaccg cgcacagagg cattttcatc
      181 gctgacaggc cgcatggcct cgcagagccg gagggggact ggtgatattc ggacgaccgt
      241 accggcttgc gccgggcttc ggcggtttta cacctgtgct acatgtaacc accgtatgcc
      301 gccttatatg cgctgactcg cattatttgc cttgttccgt ctgaaagtaa tcactatgat
      361 taaatatgat taacaactaa tcgggtatgc aaatgaaaaa caataccgca caagcaacaa
      421 aagtaattac cgcgcatgtg ccattaccta tggctgataa agtcgaccag atggccgcca
      481 gactggaacg ctcccggggc tgggttatca aacaggcgct ttctgcatgg cttgcccaag
      541 aggaggagcg taatcgcctg acgctggaag ccctggacga tgtgacatcc ggacaggtta
      601 tcgaccatca ggctgtacag tcctgggcgg acagcctcag tactgaccat ctgttaccgg
      661 tgccacgctg atggaactga agtggaccag taaggcactt tctgatttgg cgcggttata
      721 tgattttctg gtgctggcca gtaaacctgc tgccgccaga acggtgcagt ccctgacaca
      781 ggcgccggtc attctgttaa ctcatccacg tatgggagaa cagttgtttc agtttgaacc
      841 cagggaggtc agacggattt ttactggcga gtacgaaatc cgttacgaac ttactggcca
      901 gactatttat gtattgcgcc tgtggcacac acgagagaac aggtagattg ggtgtggagg
      961 caactttaac atagcctgca ggtattaatt ctggagcatt gttatcaggc cagatatttt
     1021 gcattccatt aaataatctt gtaggagata aaataaatta cgatgaaata acgaaaatta
     1081 ccgctcgtga ctggcggcaa tatcgcgctc cgggctggca aataacccat cagaaacgtt
     1141 attgccagac attgcgggta atgactccaa cttactgata gtgttttatg ttcagataat
     1201 gcccgatgac cttgtcatgc agctccaccg attttgagaa cgacagtg
//
LOCUS       DAJTRC010000273         1195 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.287, whole genome shotgun sequence.
ACCESSION   DAJTRC010000273 DAJTRC010000000
VERSION     DAJTRC010000273.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1195)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1195)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1195
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.287"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(84..>1195)
                     /locus_tag="OO357_004236"
     CDS             complement(84..>1195)
                     /locus_tag="OO357_004236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000615493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HCR5888924.1"
                     /translation="RQDNQADSVSLSVNAPEVKPEVTPSITIASLFEQYEAENYQNWK
                     PATLRENKASHAALIEIFDHLGLNADANRADMLRVRDVLQQLPRNRKQRFKDVPLADL
                     LSREDKTDCLDVVTINNKYLIKMAAVFRWAVRNDLIKKNMTEGLELKVPQRKASGARN
                     AFSTEQVGQLLVAAKAYSQKTSGKPYHYYVTALAAITGARLNEIAQLQVKDVRTTEAG
                     TVYIHINEDDSSLPGKSIKNAHSDRCVPLVDGAYGFILADFMALVETRRGADGDDAMV
                     FDGLRLMKNGYGEQVSKWFNRTLLPKVLVDRSGLAFHSFRHTVAAQLKQHGVELAYAQ
                     AIMGHSSGSITYDRYAKEVEVDRLVNVMADVYKET"
BASE COUNT          302 a          320 c          259 g          314 t
ORIGIN      
        1 cccttgacaa gtagagaaaa ctatgagtca aaaacatgac cacacaggtc tgtagtaagt
       61 gtcatcctag actatggtct cctctaggtc tccttataaa catcagccat cacattgacc
      121 agcctatcaa cctcgacctc tttagcataa cggtcgtacg taatagaccc tgaactatgc
      181 cccatgatcg cctgtgcata cgccaactcg accccatgtt gttttaactg ggccgcaact
      241 gtatgtctaa acgagtgaaa cgctaagccg ctacggtcta cgaggacttt aggcaacaac
      301 gtccgattaa accatttact cacctgctca ccgtaaccgt tcttcatgag acgcagacca
      361 tcgaacacca tagcgtcatc accgtcagcg cctctacggg tctctacaag cgccataaag
      421 tcagccaata taaaaccata ggccccatcg accagaggca cacagcggtc actatgagcg
      481 ttcttgatgc tcttaccggg tagactacta tcgtcttcat tgatatgaat ataaacggtt
      541 cccgcctcag tggtcctgac gtctttgacc tgtagttggg ctatttcatt gagtctcgcc
      601 ccagtgatag cagccaaggc ggtaacataa taatgatacg gcttaccaga ggtcttctga
      661 gagtatgcct tagctgcgac cagtagttgc cccacctgct ccgtagagaa ggcgttacgg
      721 gcccctgagg ctttacgctg agggacttta agctccagac cctcagtcat gttcttttta
      781 atcaagtcat tgcgcacagc ccacctaaag acagcagcca tcttaataag atacttgttg
      841 ttgatggtaa cgacatccag acaatctgtc ttatcttcac ggctcaacag gtcagcaagt
      901 gggacatcct tgaagcgctg cttacggttc ctaggtaact gctggagcac atcacggacc
      961 ctaagcatgt cagccctgtt agcgtctgca ttgagaccca gatggtcgaa tatttcgatt
     1021 aacgcagcgt gtgacgcctt gttttccctt aaggttgcag gcttccagtt ctgatagttc
     1081 tccgcttcgt attgttcaaa gaggctcgct atggtaatgc taggcgtaac ttctggtttt
     1141 acttctggag cattaacaga tagggacaca gagtctgctt ggttatcttg cctat
//
LOCUS       DAJTRC010000274         1146 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.288, whole genome shotgun sequence.
ACCESSION   DAJTRC010000274 DAJTRC010000000
VERSION     DAJTRC010000274.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1146
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.288"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..292
                     /locus_tag="OO357_004237"
     CDS             <1..292
                     /locus_tag="OO357_004237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888925.1"
                     /translation="TLKKSDYLPSDITRQVQQSVLDAFNGTDGGLRARVASVVSAGRY
                     YAGIYKTDPEHIDILGLTVSRDGSSWTTAVTFGIDEIPVLDVSDISVKLQEA"
     gene            294..899
                     /locus_tag="OO357_004238"
     CDS             294..899
                     /locus_tag="OO357_004238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2612 domain-containing protein"
                     /protein_id="HCR5888926.1"
                     /translation="MQNVAATVLAQYAASPRLNALINSFNAALSPDSFISDFYGLIWN
                     IDTAEKYGLDVWGKIVGVSRWLTVKDDFNYLGFSESRMDTPVMDDPCPFNQAPFYNGK
                     SDTRTVDLSDAVYRRLILMKAMSNITDCSVPDINLMLRFMFGKKRRAYVLNNGGLRMS
                     YVFESALSLAELAIIQSSGALPSPPGVYVSVVLKESRNEGQ"
     gene            886..>1146
                     /locus_tag="OO357_004239"
     CDS             886..>1146
                     /locus_tag="OO357_004239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000772810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5888927.1"
                     /translation="MKASDKPRQLAVPFASTGDKNRIPDKATQQTRESGNAAYDSGFP
                     PVTMTAVSAGGIPPHGKDFNGLMYDITAAIRFAQAGGLYTYNA"
BASE COUNT          267 a          272 c          313 g          294 t
ORIGIN      
        1 gacgctgaaa aaaagtgact acctgccttc agatattacc cgacaggtac agcaatccgt
       61 gctggacgca tttaatggta cagatggtgg tctgcgggca agagttgcct ctgttgtctc
      121 tgccgggcgc tactatgccg gcatttacaa aaccgatccg gaacatattg atattctggg
      181 ccttactgtg agccgtgatg gttcgtcatg gacaacggct gtcactttcg ggatagatga
      241 gattccggtt ctggatgtgt cggatatcag tgtgaaactc caggaggcat aacgtgcaga
      301 atgtggctgc cactgtgctt gcgcagtatg ctgccagccc ccgactcaat gccctcatta
      361 acagctttaa cgcagcgctt tcccccgaca gttttatcag tgatttttat ggtcttatct
      421 ggaacatcga taccgcagaa aagtatggtc ttgatgtctg gggaaagatt gtgggtgtca
      481 gtcgctggct gacggtaaag gacgatttta attacctggg cttcagcgag tccaggatgg
      541 acaccccggt aatggatgat ccctgtccgt ttaatcaggc accgttttac aacggaaaat
      601 cggatacccg gactgttgac ctgtctgatg ctgtataccg gcggctgata ctgatgaaag
      661 ccatgtcgaa cattactgac tgttccgttc cggatattaa cctgatgctg agatttatgt
      721 tcggaaaaaa acgccgggct tatgttctga ataatggcgg gctgaggatg agttacgtct
      781 ttgagtccgc gctctcgttg gcagaactgg cgattatcca gtcgtcgggt gcactgccat
      841 ccccgccggg tgtttatgtt tcagtagttt taaaggagtc ccgtaatgaa ggccagtgat
      901 aaaccccgcc agctggcggt cccctttgcg agtaccggag ataaaaaccg tatcccggac
      961 aaggcgacac agcagaccag agagagcggt aatgctgcgt atgattcagg ttttcctccg
     1021 gtgaccatga cagcggtctc agcgggaggt ataccgccac acggcaagga ttttaacggt
     1081 ctgatgtacg atattaccgc agcaatacgg ttcgcccagg ctggcggttt gtacacgtat
     1141 aatgcc
//
LOCUS       DAJTRC010000275         1126 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.289, whole genome shotgun sequence.
ACCESSION   DAJTRC010000275 DAJTRC010000000
VERSION     DAJTRC010000275.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1126)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1126)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1126
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.289"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..187
                     /locus_tag="OO357_004240"
     CDS             <1..187
                     /locus_tag="OO357_004240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HCR5888928.1"
                     /translation="AQDYGHRIHEFQKEMGQWVKEDKIHYREDITDGLENAPQTFIGL
                     LKGKNFGKVVIRVAGDD"
     gene            385..1050
                     /gene="mcbR"
                     /locus_tag="OO357_004241"
     CDS             385..1050
                     /gene="mcbR"
                     /locus_tag="OO357_004241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415967.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="colanic acid/biofilm transcriptional regulator
                     McbR"
                     /protein_id="HCR5888929.1"
                     /translation="MPGTGKMKHVSLTLQVENDLKHQLSIGALKPGARLITKNLAEQL
                     GMSITPVREALLRLVSVNALSVAPAQAFTVPEVRQSQLDEINRIRYELELMAVALAVE
                     NLSSQDIAELQELLGKLQQAQEKGDMEQIINVNRLFRLAIYHRSNMPILCEMIEQLWI
                     RMGPALHYLYEAINPAELRERIENYHLLLAALKAKDKEGCKHCLAEIMQQNIAILYQQ
                     YNR"
BASE COUNT          355 a          231 c          261 g          279 t
ORIGIN      
        1 cgctcaggat tatggtcacc gcatccatga gtttcagaag gagatggggc aatgggtgaa
       61 agaggataaa atccactacc gcgaagatat tactgatggt ttagagaatg cgccacagac
      121 gtttatcggc ctgctgaagg gtaaaaactt cggcaaagtg gtgatccgcg tcgcgggtga
      181 tgattaaagt ttcaacggcg gcgtaagccg ccataaaaat ccaggcaaaa agcttatttt
      241 cagctttaat taactaacca cgtcattgtt ctgctgctaa taacaacaaa ggtgggtggt
      301 ttatgattat tcactttaat acactaggtg aactccttct gccatgcagg cagggttgga
      361 cagaaaacac gcgaaggaat taaaatgcct ggaacgggaa aaatgaaaca tgtcagtttg
      421 actctgcagg ttgagaacga cctgaaacat cagcttagta ttggcgcatt aaaacctggc
      481 gcacgcctga ttactaaaaa tctggcggag caattaggta tgagtattac acctgtgcgt
      541 gaagcattat tacgtctggt ttcggtgaat gcgctttctg tcgcacctgc acaagcattt
      601 acagtaccgg aagtgaggca aagtcagtta gacgaaatca atcggatccg ctacgaactg
      661 gaattaatgg cagttgccct ggctgttgaa aacctctcct cgcaagacat agcagaactt
      721 caggaattgc tcgggaaatt acagcaggcg caagaaaagg gcgacatgga acaaatcatt
      781 aatgtgaaca ggctatttcg cttagcgatt tatcatcgct caaatatgcc catcctgtgt
      841 gagatgattg agcaactgtg gatcaggatg ggacctgctt tacattatct ttatgaagcg
      901 attaacccag cggaattacg ggagcgcata gaaaactatc atctattact cgctgcgtta
      961 aaagcaaaag acaaagaggg atgcaaacat tgtcttgctg aaattatgca acaaaatatt
     1021 gctattttat accagcaata caatcgttaa attaaatgcc cggcaaatac cgggcatcaa
     1081 gtaattactc aaatcgccac gcaatattca tgccgacacc ataatt
//
LOCUS       DAJTRC010000276         1100 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.290, whole genome shotgun sequence.
ACCESSION   DAJTRC010000276 DAJTRC010000000
VERSION     DAJTRC010000276.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1100)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1100)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1100
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.290"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            55..>1100
                     /locus_tag="OO357_004242"
     CDS             55..>1100
                     /locus_tag="OO357_004242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708400.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="invasion protein"
                     /protein_id="HCR5888930.1"
                     /translation="MREINMLRNISSCLFPHISTITSPNHYLSEWDDWEKQGLPEEQR
                     TEAVRRLRACLTSKGHKLDLRALALSSLPVLPACIKKLDVSCNKLTILTDLPENIKEL
                     IARDNFLTHISALPHYLITLDVSENQLENLPLLPDTIKSLSAEYNRLSTLPSLPLNLK
                     KLEVRNNELQTLPSLPSNLKILKVAHNHLTELPPLPRRLQLLFAYSNRLSNLPNIQEN
                     IIMRRFFYFENNQITTIPTNLFRLDPHITIEIANNPLSDQTLLFLIQQTSVPNFNGPQ
                     FRISLSDQNRLFLRQMLPQNLHSRHIRVITEGGQNFQIPPLPETVAAWFPEADRREVS
                     TQWTSFSTEENSRA"
BASE COUNT          344 a          255 c          178 g          323 t
ORIGIN      
        1 cattaggaga gatacattaa atacaattta ttcacaatct aattccatat ccatatgcga
       61 gaaattaata tgctcagaaa catttcatcc tgtttatttc cacatatcag cacaattaca
      121 tcccccaacc attatttgtc cgaatgggat gattgggaga aacaggggtt accggaagaa
      181 cagcgtactg aggcggtaag aagacttcgt gcatgtctta cctctaaggg gcataaactg
      241 gacctgcgag ccttggcgct ttcctcgcta cctgtactcc ctgcttgcat taaaaagctt
      301 gatgtgagct gtaataaatt aaccatcctt actgatttac ctgaaaatat taaagaactt
      361 attgcaagag ataatttctt aacacatata tctgcattac cacattatct aataactttg
      421 gatgtgtccg aaaatcaatt agagaatctg ccgttattac cagacaccat caaatcacta
      481 agcgcagagt ataataggtt atccacactg ccttcattac ccttgaattt aaaaaaactt
      541 gaggttagga acaacgaact gcaaactctt ccatctctgc cttctaatct taagatactt
      601 aaggttgcgc acaaccatct tactgaactg ccccctttac ctaggagact gcaacttctt
      661 tttgcatata gcaatagatt aagcaactta ccaaacatcc aagaaaatat tatcatgaga
      721 agattttttt attttgaaaa caaccaaata actacaatcc cgacaaatct ttttcgttta
      781 gatcctcata taactattga gattgcaaat aaccccttat cagatcaaac tctgctattc
      841 ttaatacagc aaacttcggt tccaaatttt aacgggcctc agtttcgtat ttccctgtca
      901 gaccaaaaca gactgttttt acgccagatg ttgccgcaaa atttacattc gcgccatatc
      961 agagtcatca ctgaaggggg gcagaacttt cagatccccc ctcttcccga aactgtggca
     1021 gcctggtttc ctgaagcaga tcgtcgggag gtttctacac aatggacttc tttttccacc
     1081 gaggagaatt cccgggcatt
//
LOCUS       DAJTRC010000277         1081 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.291, whole genome shotgun sequence.
ACCESSION   DAJTRC010000277 DAJTRC010000000
VERSION     DAJTRC010000277.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1081)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1081
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.291"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..>1081
                     /locus_tag="OO357_004243"
     CDS             <1..>1081
                     /locus_tag="OO357_004243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VIII"
                     /protein_id="HCR5888931.1"
                     /translation="NTDKQVYPLYYEAKNDKVRKRLGIKGGFYWAEAKKLSIAISRGA
                     VAIDDAGYDEDDFKKPVRVNLPVVDDLPPEGVFDTEFCNRYEKGGEDGITMVFIASSP
                     SVQDKPASTDNTNVNGEDMTEIEENMLLPVSGQELPIRWLAQHGSEKPVTHVSRDELQ
                     ALHIARDEELPAVTALAVSHKTSLLDPLEIRDLHKLVRDTDRVFPNPGNSSLGLMTAF
                     FEAYMDADYTDRGLLTKEWMKGNRVSRITRTASGANAGGGNLTDRGEGFVHDLTSLAR
                     DVATGVLARSMDVDIYNLHPAHAKRVEEIIAENKPPFSVFRDKFITMPGGMDYSRAIV
                     VASVKEAPIGIEVIPAHVTEYLNKVL"
BASE COUNT          289 a          283 c          262 g          247 t
ORIGIN      
        1 aacaccgata aacaagttta cccactgtat tacgaagcaa aaaatgacaa agtaagaaaa
       61 cgtctcggta ttaaaggcgg tttttactgg gctgaagcga aaaaattatc cattgccatc
      121 tcccgtggtg ctgttgcgat tgacgatgca ggctacgatg aagatgactt taaaaaacct
      181 gttcgcgtca atttgcccgt tgttgatgac ctcccgccag aaggcgtatt tgatacggaa
      241 ttctgcaacc gttacgaaaa aggcggggaa gatggcatca caatggtatt tatcgcatct
      301 tctccctctg ttcaggacaa accggccagc actgacaata ccaacgtcaa cggcgaagac
      361 atgacagaaa ttgaggagaa catgctcctg ccagtttccg gtcaggaact gcccattcgc
      421 tggcttgcgc aacacggcag cgaaaaacca gtaacgcacg tttcacgcga cgaactccag
      481 gcattacaca ttgctcggga tgaagaacta ccagctgtta ctgccctggc tgtttcccac
      541 aaaaccagcc tgctcgatcc gctggaaatt cgcgatctcc acaaactggt gcgtgatact
      601 gacagagttt tccctaatcc aggcaattca agcctggggc tgatgactgc ttttttcgaa
      661 gcatacatgg acgcagacta caccgatcgc ggtctgctga caaaagagtg gatgaaagga
      721 aatcgtgttt cacgcatcac gcgcacggct tccggcgcta atgctggcgg cgggaacctc
      781 accgatcgcg gcgaaggttt cgttcacgat ctgacgtcac tggcgcgcga cgtagccact
      841 ggcgtactgg ctcgttcaat ggacgtggac atttataacc ttcatccggc acacgctaaa
      901 cgtgtcgagg aaattatcgc tgaaaataaa ccgccctttt ctgttttccg cgacaaattc
      961 atcaccatgc ctggcgggat ggattattcc cgcgccatcg tggttgcgtc agtgaaagaa
     1021 gcaccgattg gtatcgaggt catccccgct cacgtcactg aatatctgaa caaggtactg
     1081 a
//
LOCUS       DAJTRC010000278         1080 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.292, whole genome shotgun sequence.
ACCESSION   DAJTRC010000278 DAJTRC010000000
VERSION     DAJTRC010000278.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.292"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            92..610
                     /locus_tag="OO357_004244"
                     /pseudo
     CDS             92..610
                     /locus_tag="OO357_004244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          329 a          242 c          227 g          282 t
ORIGIN      
        1 aatctgccgg ttacgccgaa tctgctgaat cagaacttta cgcccacagc acctaatcag
       61 gtctgggtcg cagattcagt tgtccaggcc ttccgaaatc aaccgactga aggcgctggt
      121 cgcgaaactg cagcgtatgc agttcggtaa aagctcagaa aaacttcgcg caaaaaccga
      181 acggtggata caggaagcac aggagcgaat cagcgcactt caggaggaaa tggcggaaac
      241 actgggtgag caatatgacc cggtactgcc atcttccctg cgccagtctt cagcccgtaa
      301 accgttacca gcctcacttc cccgtgcccc ccgggtcatc cggccggaag aggaatgctg
      361 tccggcctgt ggtggtgaac tcagtccact gggatgtgat gtgtcagagc agctggagct
      421 tatcagcagc gcctttaagg ttatcgaaaa acaacgccct aaactggcct gtcgccggtg
      481 cgaccatatc gtgcaggcac cggtgccttc aaaacccatt gcacgcagtt atgccggagc
      541 ggggcttctg gcccatgttg ttaccggaaa gtatgcagac catctgccat tataccgcca
      601 aagtgatcta ctattccaca cagctattta gctctctttt tgttgagctc ccatgattct
      661 ttcatttatt gttactaatt attttatgtt atcatgatgt tagaacacgc tctgctctgt
      721 gtctctattt tatctatcat attctgagga ttaactacat aacaaggcgc aagatatata
      781 tacaaaaaca aataataccg ttgcaataat tccttttagt aaaggaggtg tgattaattt
      841 caggggattt cagagtaaaa attgtacaac atattaaaat atgttcattt cgttataata
      901 cacacaagga aatattaagt tagataatat attctaaata accatagata aaaacgcaca
      961 taatggggaa tattttatga aaatacctga agcagtaaat catattaatg ttcagaataa
     1021 tattgatctt gttgatggaa aaacgaatcc gaataaagca acaaaagcat tacaaaaaat
//
LOCUS       DAJTRC010000279         1067 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.293, whole genome shotgun sequence.
ACCESSION   DAJTRC010000279 DAJTRC010000000
VERSION     DAJTRC010000279.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1067)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1067)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1067
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.293"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            70..249
                     /locus_tag="OO357_004245"
     CDS             70..249
                     /locus_tag="OO357_004245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309392.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888932.1"
                     /translation="MDLSPEDAKFLRELQHRMLREAIIEQQTGMSAIDPLRYKRASSP
                     LVDDNSNADKTRPPH"
     gene            301..495
                     /locus_tag="OO357_004246"
     CDS             301..495
                     /locus_tag="OO357_004246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888933.1"
                     /translation="MNENKIKRLEQLLQARQREFATKWGRAALPHERLTYFPLEDLIK
                     NNGKKIRSDKKHFLECNEDK"
BASE COUNT          322 a          245 c          247 g          253 t
ORIGIN      
        1 tctggagatt cagggggcca gtctaggtag cgtaaggcac tacagcgcat tgctttacga
       61 gtaataacaa tggatttatc accagaagac gcaaagtttt tacgagaact gcaacaccgt
      121 atgctcagag aagccattat cgaacagcaa acgggcatga gcgccataga cccattgcga
      181 tacaaacggg cctcatctcc tttagttgac gacaattcca atgcggataa aacacgtcca
      241 ccacactgat tacatttttc attagcacag tggtgtgatg cagcatacga ggaaaaccgg
      301 atgaatgaga acaaaataaa aaggctggag caactgttac aggcacgcca acgggagttt
      361 gccactaaat ggggacgtgc cgcgctgcca catgaacgcc taacctattt tccacttgaa
      421 gacctcatta aaaataatgg caaaaaaatc aggtctgata agaaacactt cttagaatgc
      481 aatgaagaca aataacaaca atctgcatat tgccaccaac cacagatgtg gcatcgttat
      541 cagcgcactt gattttcttt atattcactg acgagcttgc cggggcacct ttgtttgccg
      601 gtcattctgg tatcatgcag atatttacgg cgtacaagcc cactagcatg acattgtaat
      661 aaggcctgag cctaacgctt taatgtgtgt catacactca gtggcgtaat cgttgacaat
      721 atggtgcatg taatcgtaag cgtccagcga aatccatctg gtcataatct gaagcatccg
      781 acaaagtggt gtccaccaaa taagtgatct gcgcccaata tcctgagccg gaactttgac
      841 aaaggccaac gaaaaatggg agcggatgtt acagagttct cggtacaggg gaaaagtggt
      901 acctgtcgcc agttctccga tctgtttaac cgggaaatta tctcctacag cttgtcagaa
      961 aggtcggtga tggagcatcg ttaatacgat gctatacgat gcattcacag tgcccggcca
     1021 gaggatgccc cgtcgctgca tatggatcag ggttggcaat atcgaat
//
LOCUS       DAJTRC010000280         1060 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.294, whole genome shotgun sequence.
ACCESSION   DAJTRC010000280 DAJTRC010000000
VERSION     DAJTRC010000280.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1060)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1060)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1060
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.294"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..>1060
                     /locus_tag="OO357_004247"
     CDS             join(<1..153,153..>1060)
                     /locus_tag="OO357_004247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612015.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5888934.1"
                     /translation="TRLDITTHSLYAWIKKYGPDSSTNKEQSDAQAEIRRLQKELKRV
                     TDERDIFKKSRGVLRKAVRLRYAFIRDNSCCWPVRLLCRVLDVHPSGFYAWLQQPHSQ
                     RHQADLRLTGQIKQFWLESGCVYGYRKIHLDLRDSGQQCGVNRVWRLMKRVGIKAQVG
                     YRSPRARKGEASIVSPNRLQRQFNPDAPDERWVTDITYIRTHEGWLYLAVVVDLFSRK
                     IIGWSMQSRMTKDIVLNALLMAVWRRNPEKQVLVHSDQGSQYTSHEWQSFLKSHGLEG
                     SMSRRGNCHDNAVAESFFQLLKRERIKKKIYGTREEARSDIFDYIEMFYNSKRRHGSS
                     EQMSPTEYENQYYQRLGSV"
BASE COUNT          283 a          257 c          284 g          236 t
ORIGIN      
        1 caacacgtct cgatatcacc acccacagcc tttatgcctg gataaagaag tacggtccgg
       61 attcttccac taataaagaa cagtcagatg ctcaggccga gatccgccgt ctccagaaag
      121 agctgaaacg ggttaccgac gaacgggaca tattaaaaaa agccgcggcg tacttcgcaa
      181 agctgtccga ctgaggtacg cctttatccg tgacaactcc tgttgctggc ctgttcgcct
      241 gctctgtcgg gtgctggatg ttcatcccag tggtttttac gcctggcttc agcagccgca
      301 ttcacaacgc catcaggcag acctgagact gacaggacag attaaacagt tctggctgga
      361 atcgggatgc gtctatggtt atcgcaaaat ccatctggat ctgcgtgaca gcgggcaaca
      421 gtgcggagta aacagagtct ggagactgat gaaacgtgtc ggaataaagg ctcaggtcgg
      481 ataccgaagc ccgcgggcac gtaaaggcga ggccagtatc gtgtcaccca acaggctcca
      541 gcgacagttc aatccggatg ctcctgatga gcgttgggta acggacataa cctacatcag
      601 gacccacgaa ggctggctgt atcttgccgt tgttgttgat ctgttctcac gcaaaattat
      661 cggctggtcc atgcaatccc ggatgacaaa ggacattgtc ctgaacgcac tgctgatggc
      721 tgtatggcgg cgtaatcccg aaaaacaggt gctggttcat tcggatcagg gcagtcagta
      781 cacaagccat gagtggcagt cgttcctgaa atcacacggc ctggagggta gcatgagccg
      841 tcgcggtaac tgccatgata atgcggttgc agaaagtttt ttccagttgt tgaaacgtga
      901 acggataaag aaaaagatct acggaacgcg ggaagaagcc cgcagtgata tttttgatta
      961 catcgaaatg ttttataaca gtaagcgtcg gcatggttct agcgaacaga tgtcaccgac
     1021 agaatatgaa aaccagtatt atcaacggct cggaagtgtc
//
LOCUS       DAJTRC010000281         1059 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.295, whole genome shotgun sequence.
ACCESSION   DAJTRC010000281 DAJTRC010000000
VERSION     DAJTRC010000281.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1059)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1059
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.295"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            126..995
                     /locus_tag="OO357_004248"
     CDS             126..995
                     /locus_tag="OO357_004248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjeJ family protein"
                     /protein_id="HCR5888935.1"
                     /translation="MAISIKGVNTGVIRKSNNFIALALKIKEPRNKESLFFMSVMELR
                     DLLIALESRLHQKHKLDAAARLQYEQARDKVIKKMAENIPEILVDELKNADINRRVNT
                     LELTDNQGENLTFVLTLHDGSKCELVVNELQIEMLARAIIHAINNAEMRELALRITSL
                     LDFLPLYDVDCQDNGNLEYDTYSQPEWKHNLFDHYLAVLYRFKDESGKEQFSGAVVKT
                     REATPGKEIEAITRRMLDFSPRLKKLAGVPCQVYVRTVAANNAQPLTQDQCLRALHHL
                     RVQSTSKTAPQAK"
BASE COUNT          323 a          226 c          236 g          274 t
ORIGIN      
        1 ttctgtttat atagagtgag cacggctatt tatgttattt taacaactat tactaccaag
       61 cccatttatc tggcttataa attaaaatta attatttaat tgagaaatgg ttagggagaa
      121 cctacatggc cataagcatc aagggtgtta ataccggtgt tattcgcaag agtaataatt
      181 tcattgcact ggcgctgaaa attaaagaac ctcgcaataa agaatcgttg ttctttatgt
      241 ctgtcatgga actgcgcgac ctgctgattg cgctggaaag tcgtctgcat caaaagcata
      301 aactggatgc tgctgcacgc cttcagtatg agcaagctcg cgataaagtc attaagaaaa
      361 tggcagaaaa catcccagaa attctggttg atgagcttaa aaatgccgat attaatcgtc
      421 gggttaacac gctggaactt accgataatc aaggcgaaaa tcttaccttc gtattaacgc
      481 tacatgacgg tagcaaatgt gagttagtcg tcaatgagtt gcagattgaa atgctggcgc
      541 gagcaatcat tcatgcaatt aataatgcgg aaatgcgtga actggcatta cgtattacct
      601 cactgttaga tttcctgccg ttatacgacg ttgactgcca ggacaatggc aatctggaat
      661 atgacaccta ttcccaaccg gaatggaaac ataacttgtt cgaccattat ctggcggtgc
      721 tttaccgctt taaagatgaa agtggcaaag aacaatttag cggcgcagtg gtgaaaacgc
      781 gtgaagcaac gccgggtaaa gaaatagagg cgatcactcg ccgaatgctt gatttcagcc
      841 cacgactgaa aaaactcgcg ggtgtgccat gtcaggtcta tgttcgcacc gtagcggcga
      901 ataacgccca accgctaacc caggatcaat gcttacgcgc gctgcatcac ttacgtgtac
      961 aatccaccag caaaactgca ccacaggcga aataatctca tctttgctaa tgcgaggctg
     1021 gagccatcca gcctgaaatg actgataacg tgatcgttg
//
LOCUS       DAJTRC010000282         1023 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.296, whole genome shotgun sequence.
ACCESSION   DAJTRC010000282 DAJTRC010000000
VERSION     DAJTRC010000282.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.296"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            66..413
                     /locus_tag="OO357_004249"
                     /pseudo
     CDS             66..413
                     /locus_tag="OO357_004249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416381.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
     gene            768..1016
                     /locus_tag="OO357_004250"
     CDS             768..1016
                     /locus_tag="OO357_004250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinI-like family protein"
                     /protein_id="HCR5888936.1"
                     /translation="MRFEICIAKEKMTKMPTGAVDALKEELTRRISKRYDDVEVIVKA
                     TSNDGLSVTRTADKDSAKTFVQETLKDTWESADEWFVH"
BASE COUNT          299 a          239 c          232 g          253 t
ORIGIN      
        1 atgcgcgacg ggacttttta taccccgcag gggtcataac atgcttgaca acttaatagg
       61 ggcactcccc cgctaaagtg ttgcccgaaa actggtggca acatccagct gcattaggtg
      121 caactgacag tgatatcgaa atcatcaaac gtcagtgggg tgcgttttac ggtacggatc
      181 tggagttgca actgcgccgc cgaggtatcg atacaatagt gttatgtggg atctcgacca
      241 atatcggtgt tgaatccacc gcccgcaatg cctgggaact tggctttaat ctggtgattg
      301 ccgaagacgc ctgtagtgcc gccagcgccg agcaacacaa taacagcatt aatcatatct
      361 acccgcgcat cgcccgtgtg cgtagcgtgg aagagatcct tcacgcgtta tgatttacat
      421 cggtctaccg caatggtcac atcctaaatg ggtgcggttg gggatcacca gccttgaaga
      481 gtatgcccgc cactttaact gcgtgatacg ggatttttaa aaatcactaa agaacgccca
      541 agagcatgtg ttttctttag aataatcaaa gcgttggatt gccatgtcat atgaaaatcg
      601 gcaaagttca tatgtaacaa agtgtcccaa tcatgcccca aaatgcccca caacaaatat
      661 ttttgcccca tgcatgcccc aaaaaatcac tttccctcac tcttgacact gtttatccat
      721 acagttaaaa ataatactgt ataaaaacac agtgcagagg gatttttatg cgttttgaaa
      781 tctgcatagc caaagagaaa atgactaaaa tgccaaccgg tgctgtggat gcgttaaagg
      841 aagaattaac ccgacgcatc agtaaacgtt atgacgatgt agaggtgatc gtaaaagcca
      901 ccagcaacga tggcctttct gttacacgca ccgcagataa ggattctgca aaaacatttg
      961 ttcaggagac tctgaaagat acctgggagt ctgctgacga gtggtttgtt cactaataaa
     1021 cac
//
LOCUS       DAJTRC010000283         1010 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.297, whole genome shotgun sequence.
ACCESSION   DAJTRC010000283 DAJTRC010000000
VERSION     DAJTRC010000283.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1010)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1010)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1010
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.297"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(8..727)
                     /gene="yehT"
                     /locus_tag="OO357_004251"
     CDS             complement(8..727)
                     /gene="yehT"
                     /locus_tag="OO357_004251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system response regulator BtsR"
                     /protein_id="HCR5888937.1"
                     /translation="MIKVLIVDDEPLARENLRIFLQEQSDIEIVGECSNAVEGIGAVH
                     KLRPDVLFLDIQMPRISGLEMVGMLDPEHRPYIVFLTAFDEYAIKAFEEHAFDYLLKP
                     IDEARLEKTLARLRQERSKQDVSLLPENQQALKFIPCTGHSRIYLLQMKDVAFVSSRM
                     SGVYVTSHEGKEGFTELTLRTLESRTPLLRCHRQYLVNLAHLQEIRLEDNGQAELILR
                     NGLTVPVSRRYLKSLKEAIGL"
     gene            complement(724..>1010)
                     /locus_tag="OO357_004252"
     CDS             complement(724..>1010)
                     /locus_tag="OO357_004252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HCR5888938.1"
                     /translation="LQPIVENAIKHGTSQLLDTGRVAISARREGQHLMLEIEDNAGLY
                     QPVTNASGLGMNLVDKRLRERFGDDYGISVACEPDSYTRITLRLPWRDEA"
BASE COUNT          243 a          289 c          215 g          263 t
ORIGIN      
        1 cagtctttta caggccaatc gcctctttta agcttttcag ataacggcgg ctgaccggca
       61 cggttaagcc attacgcaaa atcaactcgg cctggccgtt atcttccaga cgaatctcct
      121 gtaaatgcgc gaggttaacc agatactgac gatggcagcg cagtagtggt gtacgacttt
      181 ccaaggtacg taatgtcaat tcggtaaagc cctctttccc ttcgtggctg gtaacgtaga
      241 caccgctcat ccgactgctg acaaatgcca catctttcat ttgcagcaaa taaatccgac
      301 tatgccccgt acaagggata aatttcagcg cctgttgatt ttccggtaac agcgaaacat
      361 cctgcttgct gcgctcctga cgcaatcgcg ccagcgtttt ctccagtcgc gcttcatcaa
      421 ttggcttcag cagataatca aaggcatgtt cttcaaaggc tttaatagcg tattcgtcaa
      481 acgcagtgag aaaaacaata tacgggcgat gttccgggtc aagcatcccc accatttcca
      541 gaccactgat gcgcggcatc tggatatcga gaaacagcac atccgggcgc agtttatgca
      601 ccgcgccgat cccttccacg gcgtttgaac actctccaac gatttcaata tcgctctgct
      661 cctgcaaaaa tatacgcagg ttctcccgtg ctaacggttc atcatcgaca attaagactt
      721 taatcatgcc tcgtccctcc atggtagtcg taacgttatt cgggtgtaac tatcaggctc
      781 acaggcgacg cttattccat agtcatcgcc aaaccgttca cgtaaacgct tatccaccag
      841 attcatcccc agcccactgg cattggttac cggttgatac aaaccggcat tgtcttcgat
      901 ctccagcatc aaatgttgcc cctcacgtcg ggcgctgatt gccactcgcc ctgtatccag
      961 cagttgtgat gtcccatgtt taatggcgtt ttccactatc ggttgcaggg
//
LOCUS       DAJTRC010000284         1000 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.298, whole genome shotgun sequence.
ACCESSION   DAJTRC010000284 DAJTRC010000000
VERSION     DAJTRC010000284.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.298"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(142..478)
                     /locus_tag="OO357_004253"
                     /pseudo
     CDS             complement(142..478)
                     /locus_tag="OO357_004253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_009502602.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          332 a          180 c          178 g          310 t
ORIGIN      
        1 agaacttctt cagcacactg ctaaaacgca tagtgatcga gcgctgattc tggcgaacaa
       61 ctgaactaat acatcagaat ctgcattatg ttaaataaat atgaaaagat ggtttaaata
      121 ccccgttact tttgacttac actatacggt atcgcatcgt ttaatattcg caccggccag
      181 atttttattt ctattagttg tcacaatact gaatgcgtac gaccacagta ttctggctcc
      241 tgtgtggtta tgctttaatt ctgcgttccg ggcagataag cagttgcttg caggaatcct
      301 tcttgtgtta atgtcagttc ccctttttac cagtgctgat ttccacattc cgtccaacag
      361 agcttatagc ctttccctgg attatagcat tgtccggctg aagttctttt tgaataataa
      421 tagaagcact gctggcagat ccagtccgtt tttcataacc cactgtactg ataaccataa
      481 tctaatcagt agaaattgag tcgaaaataa gcactactct atacaggata attagaggtc
      541 agtttgatta ttcacaattc atcatcagca ttttctattt ctgacgaaat caacatgaaa
      601 ataaccatat atgataatta ttataataac ggctttaatt ggaatacata tattacaacg
      661 tattatatat aattggtatt ctgggaacta tattctcaaa atacagtaga aacgaggtat
      721 gtttctggtg gaaaggacag tgggattaaa aagtaagaat tgataaaaaa aacgccagca
      781 acacatagtg ccggcggagg gaatacccca tggagaaaat gtgatgccta gagcatcatg
      841 aatatataat taaaaatagt tagcgttgtc aatactttca caaaaataat tttcgtagta
      901 tagaaagata tttttatgca tgacctacct gaatttgctc cgggtagagg ttataaataa
      961 aaattgaatc acgacaaaca caatattcat agtatggcga
//
LOCUS       DAJTRC010000285          996 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.299, whole genome shotgun sequence.
ACCESSION   DAJTRC010000285 DAJTRC010000000
VERSION     DAJTRC010000285.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..996
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.299"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..156
                     /locus_tag="OO357_004254"
     CDS             <1..156
                     /locus_tag="OO357_004254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-packaging protein FI"
                     /protein_id="HCR5888939.1"
                     /translation="LVTVVALVTLHTDALHATRDEPLAFVPPGMAFRVFAGVAAGMTE
                     RGLARMQ"
     gene            168..539
                     /locus_tag="OO357_004255"
     CDS             168..539
                     /locus_tag="OO357_004255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5888940.1"
                     /translation="MADFDNLFDVALDLADKAIIRNMGIRAVITSGRLKGIMISGVFD
                     DPENISLVAGGVRIEDSLPSLFVKTADILRLCRNDSLMIGRESFCVDRITPDDGGCSY
                     IRLRREGLPGNVRAGRYYEGA"
     gene            526..>996
                     /locus_tag="OO357_004256"
     CDS             526..>996
                     /locus_tag="OO357_004256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5888941.1"
                     /translation="MKGLENAIRNLNSLDTRMVPQASAWAINRVAQKAVSVATRQVAG
                     NTVAGDNQVKGIPLKLVRQRVRVFKASPSGKMTARIRVNRGNPPAIKLNTTRRRAGEG
                     LRVGKYFFRGAFVQQLANGRWHVLRCLPEARFATGHDHQGRLRKNRLPVEVVKIP"
BASE COUNT          223 a          227 c          309 g          237 t
ORIGIN      
        1 ctggttacag tcgtggcact ggtgacgctg cataccgatg cacttcatgc cacgcgggat
       61 gaacctctgg catttgtgcc gccgggaatg gcgttccgtg tctttgccgg tgtggcagcc
      121 ggaatgacag aacgtggcct ggccagaatg caataacggg aggcagcgtg gctgatttcg
      181 ataatctgtt tgatgttgca cttgatctcg cagacaaggc cattattcgc aatatgggga
      241 ttagagcggt cattacgtca ggccggctaa aagggatcat gatttccggg gtttttgatg
      301 atcctgaaaa tatttctctg gtggccggcg gtgtgcgtat tgaagactct ttaccatccc
      361 tgtttgtgaa aacagcagat attttacggc tgtgtcgcaa tgattcgctg atgattggtc
      421 gtgagtcttt ctgtgtggat cgtatcaccc ctgatgatgg cggatgtagt tatatccggt
      481 tgcggcgtga ggggctgccg ggaaacgtaa gggcaggacg atattatgaa ggggcttgag
      541 aatgccatcc gcaatctgaa cagccttgat acccgtatgg tgccacaggc cagcgcatgg
      601 gcgataaacc gtgtggcaca gaaagcggtc tcggttgcca cccggcaggt tgccgggaat
      661 accgttgcgg gcgataacca ggtgaaaggg atccccctga aactggtacg tcagcgtgtc
      721 cgggtgttta aagccagtcc gtcaggaaaa atgacggcca ggatccgcgt taaccggggc
      781 aatccgcccg ccatcaaact gaacacaaca cggcggcgtg ctggtgaagg actgagagtg
      841 ggaaaatact ttttccgggg ggcatttgtt cagcaactgg cgaatggccg ctggcatgtt
      901 ctgaggtgtc ttcctgaagc gcgttttgca acagggcatg accatcaggg caggctaaga
      961 aaaaatcgtc ttcctgtgga ggtagtgaaa atcccg
//
LOCUS       DAJTRC010000286          989 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.300, whole genome shotgun sequence.
ACCESSION   DAJTRC010000286 DAJTRC010000000
VERSION     DAJTRC010000286.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 989)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..989
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.300"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(1..908)
                     /locus_tag="OO357_004257"
                     /pseudo
     CDS             complement(1..908)
                     /locus_tag="OO357_004257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS4-like element ISSfl1 family transposase"
BASE COUNT          255 a          228 c          206 g          300 t
ORIGIN      
        1 aaatccgaag cgcccgttct tttcatcacg aaagttttgc tctatctgca ttcgccgact
       61 gtataacttg atgatttctc gcgctctgaa gtcatttgta ctgctgaaga tcaaccaggc
      121 ttcttttgct gatttgctct gttctttgtc tgttttgttt aaaccactct gacctttcga
      181 acgtttcttt tttcgccctt ttgccgattt cttataggtg taaaaatgcc cccggatgct
      241 tttttccttt ctttaaccag ccttccagcc cccatatatt ccggtgtttt acattcaggg
      301 ctatccgaca cttttaacca tctttcaggc gcgttatcga ggcaatactg aacgttattt
      361 ctgatccgac caataaaatc ccagcccaga gaagtaatat ggtgaaacca tgcgctctga
      421 aatccagcat ccgtgacaat aataaccctg gcatcggggg gcagggattg agcaagggaa
      481 tcaaggaaat catgttgtat caatggatta ttttgttttt cagaaggaac aaccttactc
      541 aataacggaa tggaacgccc gtcacacaga aggctggcac gaagaacatg atgctcctgg
      601 gagggatagc cactccagtc tacggctatt acacataaag ataactgccg tgtcagcatt
      661 gaagtgatat tcctgaaaat cattggaata tcgcgatgga gcgcctcatt acccatgagg
      721 cgatcaatac gtttgatctt attcttaact tgtgcattac ccggcaaaaa acgtccgata
      781 ctggtctgcg tcagagatgc tccgttgatt aacgccattg tggcatccat cagggcattt
      841 tgtcggtact gatgaaatgg tgctaaggca tcccggaaga aattctgaca tactttatga
      901 acaggcatag cagtgatctc attgaattgt tggcacaaat cagtagatca cataatgcta
      961 tgtttgtctc gttttctggg gattcctca
//
LOCUS       DAJTRC010000287          986 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.301, whole genome shotgun sequence.
ACCESSION   DAJTRC010000287 DAJTRC010000000
VERSION     DAJTRC010000287.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 986)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 986)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..986
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.301"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
BASE COUNT          298 a          225 c          204 g          259 t
ORIGIN      
        1 cccacccaaa ccaataagga ctaacatgac ttttaactgt gccgcgtgcg catagccgca
       61 ttcaccgcat cacaaaattc actttaaaaa gggcggacat cagccagcaa tgaaactgat
      121 gccgccaaag gtaccaatca acatggagtg ttgtggcgtg gttgtcactt aagcgtatgg
      181 tcaacctgac aacccggtgt cctcaacggg gaaggaataa ctccgccata cttaccgccg
      241 cgtcatttcg cgttgtgtgc ctgcttttaa ctacgtcagg cgaggtggtt cctgttattc
      301 cccaacaaca agaaatttgt ataatctgaa taccccaaca ataagaagag tgagcaagtg
      361 atgtattagc gatgtcagct ctcaaagaga ctgcatcaat gttaaaatta ttcaatgaag
      421 caaaaactca ggctgaggtt gatcgcgctg tgtctgaaat aaactctaag ttgtcaagca
      481 ttcagcttga atatttgtca tctcattgat gagctgacaa tctctaaaag caatgaggct
      541 gcgctcaaag caaaactcac ggagatagaa aagtttaagt tccaaagtga agactgcgaa
      601 cctcgctaat tgattactgg aacgattgtg tatgccagac agtgcaatac tagctctggc
      661 aagattacca tgtatttttg tccagaatgt tttgcgaaga gagcaatatc tatattgcaa
      721 cctttcccta tcgaggaagg cgataaatat acgaaaactc aatgcccctc ctgtaaaaat
      781 gagtatctca tggactacaa tttcaataat gatttttgag tcacggtatt cagattttta
      841 aagaacatgc cggatactca cccgtgtccg gcgcacgcac tccacctcac ccgtggagaa
      901 ctccttaatt accaaccttg gcttcgttgg ttagccatta acgctggtat gtaatcattc
      961 tggcaatgct taatttgaag tggcac
//
LOCUS       DAJTRC010000288          983 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.302, whole genome shotgun sequence.
ACCESSION   DAJTRC010000288 DAJTRC010000000
VERSION     DAJTRC010000288.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 983)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..983
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.302"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO357_004258"
     CDS             complement(20..823)
                     /gene="dkgB"
                     /locus_tag="OO357_004258"
                     /EC_number="1.1.1.346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,5-didehydrogluconate reductase DkgB"
                     /protein_id="HCR5888942.1"
                     /translation="MAIPAFGLGTFRLKDDVVISSVKTALELGYRAIDTAQIYDNEAA
                     VGQAIAESGVPRHELYITTKIWIENLSKDKLIPSLKESLQKLRTDYVDLTLIHWPSPN
                     DEVSVEEFMQALLEAKKQGLTREIGISNFTIPLMEKVIAAVGAENIATNQIELSPYLQ
                     NRKVVAWAKQHGIHITSYMTLAYGKALKDEVIARIAAKHNAIPAQVILAWAMGEGYSV
                     IPSSTKRKNLESNLKAQNLQLDAEDKKAIAALDCNDRLVSPEGLAPEWD"
BASE COUNT          259 a          220 c          222 g          282 t
ORIGIN      
        1 gaggagctgt cagagaggct taatcccatt caggagccag accttccggg ctaaccaggc
       61 ggtcgttgca atccagtgcg gcgatcgctt ttttatcttc ggcatcaagc tgtaaatttt
      121 gtgccttaag attactttcc aggtttttac gtttagtaga agaaggaatt actgagtaac
      181 cttcccccat agcccacgcc agaatcactt gtgccggaat cgcattgtgt ttagctgcaa
      241 tacgagcaat aacctcatct ttcagtgcct taccataagc cagcgtcata taggaagtga
      301 tatgaatacc gtgctgttta gcccaggcaa ccactttacg gttttgcaga taaggagaga
      361 gttcaatctg gttggtagcg atgttttcag cgccaacagc agcaatcact ttttccatca
      421 atgggatcgt gaagttggaa ataccgatct cacgcgtcag cccttgtttt ttggcttcca
      481 gcagcgcctg cataaactct tcaacagaga cttcatcgtt tggtgacggc cagtggatta
      541 gcgtcagatc aacataatcg gtacgcaatt tttgcaggct ctctttcaga ctcgggatca
      601 atttgtcttt gctgagattt tcaatccaga ttttagtggt gatgtagagt tcatgacgtg
      661 gcacgccact ttctgcaatc gcctgaccta ctgcggcttc gttatcatag atttgtgcgg
      721 tatcaattgc gcgataacca agttcaagcg ccgttttcac agatgaaata acaacgtcgt
      781 ctttcagacg gaaagtacct aaaccaaatg cagggatagc cataatatgc ctcttttaat
      841 gctattttcg ttaactgagg tgattatggg cttcagactg ttgcgggaaa agagcgtaaa
      901 acgcagagga tttttgcgat tctagcaata atagatatcc agacgcaaaa aaacatcgag
      961 cgttaactcg aggcttctta ata
//
LOCUS       DAJTRC010000289          946 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.303, whole genome shotgun sequence.
ACCESSION   DAJTRC010000289 DAJTRC010000000
VERSION     DAJTRC010000289.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 946)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 946)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..946
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.303"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..>946)
                     /locus_tag="OO357_004259"
     CDS             complement(<1..>946)
                     /locus_tag="OO357_004259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013747467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5888943.1"
                     /translation="PRFADIFQQGNRWLNWLEKQPEGAVRPVVIESVTKIMACGTTLM
                     GYTQWCCSSPDCCHTKKVCFRCKSRSCPHCGVKAGAQWIQYLLSLVPYCPWQHIVFTL
                     PCQYWSLVFHNRWLLAEMSRIAADVILEICHQADVEPGIFTVIHTWGRDQQWHPHIHL
                     STTAGGVTSGHTWKNLHFYARKVMSMWRYRITRLLSRKYPDLVMPDALAAEGSSKREW
                     NRFLDTHYRRGWNVNVSRVMDNTTHVAVYFGSYLKKPPVPMSRLEHYAGQDEIGLRYN
                     SHRTKREEYLLMSGDESMERFSWHVADKGFRMVRYYVF"
BASE COUNT          217 a          279 c          231 g          219 t
ORIGIN      
        1 aaaaaaacgt agtacctcac catacggaac cccttatccg ccacatgcca ggagaacctt
       61 tccatggact catctccact catcaacagg tattcttccc gttttgttcg gtgactgttg
      121 taacgcagac cgatttcatc ctgaccggca taatgctcca gacgactcat cggcaccggt
      181 ggctttttca ggtaagagcc aaagtacacc gccacatggg tggtattatc cattacccgg
      241 gatacgttga cattccagcc acggcggtaa tgcgtgtcca ggaagcgatt ccattcccgt
      301 ttactgcttc cttctgctgc cagcgcatcc ggcatcacca ggtcagggta tttccgtgac
      361 agcaaccgtg ttatccggta gcgccacatg ctcatcacct tacgggcgta aaaatgaaga
      421 tttttccagg tgtggcccga cgtcacacca ccggcagttg tcgataaatg gatatgcgga
      481 tgccactgct ggtcacgccc ccatgtgtgg atcaccgtga atatccccgg ctccacatct
      541 gcctgatggc agatttccag tatcacatcc gctgcaatgc ggctcatctc tgccagtaac
      601 caccggttgt ggaacaccag ggaccagtac tggcagggaa gtgtgaacac aatatgctgc
      661 cacgggcagt aggggaccag gctcagcaga tactgtatcc actgtgcgcc agccttcacc
      721 ccgcagtgcg ggcaggagcg gcttttacac cggaagcaga ccttttttgt atggcaacag
      781 tccggtgatg aacaacacca ctgcgtgtag cccatcagtg tggtcccgca ggccatgatt
      841 tttgtcactg attcaattac caccggacgg actgcccctt ccggttgttt ctccagccag
      901 ttaagccagc ggtttccctg ctgaaaaata tcggcaaaac ggggga
//
LOCUS       DAJTRC010000290          931 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.304, whole genome shotgun sequence.
ACCESSION   DAJTRC010000290 DAJTRC010000000
VERSION     DAJTRC010000290.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 931)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 931)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..931
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.304"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..269
                     /locus_tag="OO357_004260"
     CDS             <1..269
                     /locus_tag="OO357_004260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5888944.1"
                     /translation="SQYEDGLLSDDDIQECMPVPVALTDTPEPTRPVTNPFWRNKHDE
                     RPEGAPGNVPRCELHLKSGVVKLFDPLTPELLRALIREMKGGIR"
     gene            266..616
                     /gene="tnpB"
                     /locus_tag="OO357_004261"
     CDS             266..616
                     /gene="tnpB"
                     /locus_tag="OO357_004261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HCR5888945.1"
                     /translation="MITLPTGTKIWIIAGITDMRCGFNGLASKVQNTLKDDPFSGHIF
                     VFRGRSGKMVKILWADRDGLCLFTKRLAGDPGRESAPDASSVIHATGGDRVATSQTDR
                     TAWHPDITRDKTRE"
     gene            613..>931
                     /locus_tag="OO357_004262"
     CDS             613..>931
                     /locus_tag="OO357_004262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HCR5888946.1"
                     /translation="MNNELPDDIELLKAMLRKQQSRLRQYACQVAGYEQEIERLKAQL
                     DRLRRMLFGQSSEKKRHKLENQIRQAEKRLSELENRLNTARNLLEDASSVTDSPDTSP
                     PSEN"
BASE COUNT          253 a          238 c          258 g          182 t
ORIGIN      
        1 aaagccagta cgaggacggc ttactgagcg atgatgacat acaggaatgc atgcctgtcc
       61 cggtggcact gactgatacg ccggagccga ccagaccagt tacaaatccc ttctggcgta
      121 acaagcatga tgagcgccct gagggggctc ccggaaacgt cccacggtgc gagctgcatc
      181 ttaaatcagg tgtggtaaaa ctgtttgacc ctctcactcc ggaactgtta cgggcgctaa
      241 tccgcgaaat gaaagggggt atccgatgat aacgctgccg accggtacca aaatctggat
      301 catcgctggc atcacagata tgcgttgtgg cttcaatggc ctggcttcga aggtgcagaa
      361 cacgctgaaa gatgacccgt tctccgggca tatcttcgtc ttccggggcc gcagtggcaa
      421 aatggtgaaa atactgtggg ccgatcgtga cgggttatgc ctgttcacca aacgtctggc
      481 cggtgacccg ggaagggaaa gtgcacctga cgccagctca gttatccatg ctactggagg
      541 ggatcgcgtg gcaacatccc aaacggacag aacggcctgg catccggata taacccgtga
      601 taaaacaagg gaatgaacaa tgaactcccc gatgatattg agctgcttaa agccatgttg
      661 cgtaagcaac agagtcggct tcgacagtat gcctgtcagg tcgcgggcta tgagcaggaa
      721 attgaacggc tgaaagcgca actcgacagg ttgcgtcgta tgttgttcgg ccagagttca
      781 gagaaaaagc gtcataagct tgaaaatcag atccgacagg cagaaaaacg actgtcggaa
      841 ctggaaaacc ggctgaacac agccagaaat cttctggaag atgcatcgtc agtcacagat
      901 tcacctgaca ccagtccccc gtcagaaaac c
//
LOCUS       DAJTRC010000291          921 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.305, whole genome shotgun sequence.
ACCESSION   DAJTRC010000291 DAJTRC010000000
VERSION     DAJTRC010000291.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..921
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.305"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(144..>921)
                     /locus_tag="OO357_004263"
     CDS             complement(144..>921)
                     /locus_tag="OO357_004263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005121390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5888947.1"
                     /translation="LTAKERFTALQSGEVDLLSRNTTWTSSRDAGMGMAFTGVTYYDG
                     IGFLTHDKAGLKSAKELDGATVCIQAGTDTELNVADYFKANNMKYTPVTFDRSDESAK
                     ALESGRCDTLASDQSQLYALRIKLSNPAEWIVLPEVISKEPLGPVVRRGDDEWFSIVR
                     WTLFAMLNAEEMGINSQNVDEKAANPATPDMAHLLGKEGDYGKDLKLDNKWAYNIIKQ
                     VSNYSEIFERNVGSESPLKIKRGQNNLWNNGGIQYAPPVR"
BASE COUNT          221 a          240 c          224 g          236 t
ORIGIN      
        1 tctggaataa ccaggcacga accgtagggt tggcaaagga gagtgagcct ttaacggttg
       61 agcggcgatg aggcatagaa acctcggaaa gcataacgct gaagagagcg ccgccgtagc
      121 gacgctcgtt acctcacacc gacttaacgc acgggcggcg cgtactgaat accgccgtta
      181 ttccagagat tattttgccc acgtttaatt ttcagcgggc tttctgaacc tacgttacgc
      241 tcaaaaattt ccgagtagtt actcacctgt ttgatgatgt tataggccca tttattatcc
      301 aacttcagat ccttgccgta atcgccttct ttacccaaca gatgtgccat atcaggcgtt
      361 gctggattag ccgctttttc atcgacgttc tgggaattga tgcccatctc ttcagcattt
      421 agcatggcga aaagcgtcca gcgtacaatc gagaaccatt catcatcgcc acgacgaact
      481 accggcccaa gcggttcttt agagataacc tccggtaaga caatccattc agccgggttg
      541 cttaatttga tgcgcaaggc atacagttgc gactgatccg aagccagcgt atcgcagcga
      601 ccagattcca gtgccttcgc tgattcgtca gagcgatcga aagtcactgg tgtgtacttc
      661 atattgtttg ccttgaaata gtcggcgacg ttgagttcgg tatcagtacc cgcctgaata
      721 cagacggtag cgccatccag ttctttcgcg ctttttagcc ccgctttatc gtgcgtcagg
      781 aagccaatgc cgtcgtaata agtgacgccg gtaaatgcca ttcccatccc ggcatcgcga
      841 gatgaagtcc aggtcgtatt acgggagagc aaatccactt cccctgactg taaagcggtg
      901 aagcgttctt ttgctgtgag t
//
LOCUS       DAJTRC010000292          919 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.306, whole genome shotgun sequence.
ACCESSION   DAJTRC010000292 DAJTRC010000000
VERSION     DAJTRC010000292.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 919)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 919)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..919
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.306"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            98..>919
                     /locus_tag="OO357_004264"
     CDS             98..>919
                     /locus_tag="OO357_004264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001007947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase family protein"
                     /protein_id="HCR5888948.1"
                     /translation="MAISDTKLRTIYGKPYSGPQEVADADGLSVRISPKGVIQFQYRY
                     RWHGKPNRLGLGRYPSLSLKDARQITADLRKLYFSGTDPRTYFEEKVENSMTVAQCLD
                     YWFDNYVSTTLREKTQALYRSTVMKRMHDAFPNRPASSITVKQWVDLLTEEEKDNPRR
                     ARQVLSQLRSAISWCMRRQLIDSCAIMSIQPRDFGSRAEVGDRVLSYHELAKIWLAIE
                     RSRASTSNKLLHQMLMLWGARLSELRLATKTEFDLLDNVWTVPKEHSKMGNVIRRP"
BASE COUNT          243 a          217 c          231 g          228 t
ORIGIN      
        1 cgtcaaatat agttgtttac tgtgcgctgt tgtccatgtg atattgtgta ccagatgtgt
       61 gccaaactat tatttctgag cgtaccaaat attatttatg gctataagcg acacaaaact
      121 gcgtactatt tatggtaaac catattcggg cccacaagaa gtggctgatg ccgatggcct
      181 cagcgtacga atttcaccga agggggtcat ccagttccag taccgctatc gctggcatgg
      241 caagcctaat cgacttgggc ttggtcgata cccatccctg tctttgaagg atgccagaca
      301 gatcactgct gacttgcgaa agctctattt ctcaggaacg gatccacgca cctattttga
      361 agagaaggtg gagaactcca tgacggtcgc ccagtgtctc gactactggt tcgacaacta
      421 cgtctctaca actctcagag aaaagaccca ggcactttat cgatcaacgg ttatgaagcg
      481 catgcatgac gccttcccta atcgtccggc atcttctatc acggttaagc aatgggttga
      541 cctgcttacc gaagaagaaa aagataatcc acgccgagca aggcaggtgc taagtcaact
      601 aagatcagca attagttggt gcatgcggcg tcagttgata gatagttgcg caattatgag
      661 catccagcca agggacttcg gctcccgcgc tgaggtaggg gatcgggtac tgtcgtatca
      721 cgaactggct aagatttggc ttgctattga aagaagccgt gcgtctacgt caaataagct
      781 ccttcatcag atgcttatgc tgtggggggc gaggctctca gagcttaggc tggcaacaaa
      841 gacagaattt gacctgctgg acaacgtatg gaccgtaccg aaagagcata gcaagatggg
      901 taatgttatc cgccgtcca
//
LOCUS       DAJTRC010000293          911 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.307, whole genome shotgun sequence.
ACCESSION   DAJTRC010000293 DAJTRC010000000
VERSION     DAJTRC010000293.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 911)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 911)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..911
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.307"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            29..820
                     /gene="ltrA"
                     /locus_tag="OO357_004265"
                     /pseudo
     CDS             29..820
                     /gene="ltrA"
                     /locus_tag="OO357_004265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005137363.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
BASE COUNT          225 a          212 c          279 g          195 t
ORIGIN      
        1 tacagggacg gagtaaaccg tagtagcggt gaaggaccgt aatgggtccg gagcaaaggg
       61 cttgccccat tgtgctgatg gtgcggcggc aaccacgggc gacaatgccg atgggaggac
      121 tgctgtaaaa tcggcaaagc catttcctgt aagtaaacga caggtatggg aagcttataa
      181 acgagtaaaa gccaaccgtg gggcggcagg cattgacggg cagacactgg ccgggtttga
      241 cgagaatgtt acagacaatc tctacaaact gtggaacaga atggcttccg gcagttatat
      301 gccgcaggcg gtaaggcgtg tggacattcc caaagctgac ggtggtgtac gtccgctcgg
      361 tattccggcc gtgtcggacc ggatcgccca gatggtcgtt aagcagatac tcgagccggt
      421 actggaaccc ctgttccatg cagactcgta tggttatcga ccgggtaaat ccgcacacca
      481 ggcgattgcg caggcgagga aacgctgctg gaagtttgac tgggtggtgg aagtcgatat
      541 taaaggcttc gttgatgata tcgatcatga cctgctactg aagacagtgc agcatcatac
      601 tcaggcacgc tgggtggtga tgtacattga gcgcaggctg aaggcaccgg tgcaaatgcc
      661 ggatggagca atgctggcac ggggtcgagg aacgccgcag ggcggcgtaa tctcccccct
      721 gctctcaaat ctgttcctgc actatgcctt tgatatgtgg atgcaacgtc agtttcccgg
      781 cgttcccttc gaaaggtacg ccgatgatgt tgtatgccac tctcggatat gacttcaaag
      841 tgcgtactct gaagaatttc aaaggcgaat tgtacagaaa atgtatgccg ggtgcatcaa
      901 atgcagcaat g
//
LOCUS       DAJTRC010000294          909 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.308, whole genome shotgun sequence.
ACCESSION   DAJTRC010000294 DAJTRC010000000
VERSION     DAJTRC010000294.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 909)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..909
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.308"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..>909
                     /locus_tag="OO357_004266"
     CDS             <1..>909
                     /locus_tag="OO357_004266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5888949.1"
                     /translation="NQDAFWEQMVNNELAAKKALSPAAILALEKEYANKKLAAYIFPG
                     RNLSLTVDADPQAEISSKETLAENLTSLTLSNGAKVILAKSAGEEQKLQIIAVSNKGD
                     LSFPAQQKSLIALANKAVSGSGVGELSSSSLKRWSAENSVTMSSKVSGMNTLLSVSAR
                     TNNPEPGFQLINQRITHSTINDNIWASLQNAQIQALKTLDQRPAEKFAQLMYETRYAD
                     DRTKLLQENQIAQFTAADALAADRQLFSSPADITFVIVGNVAEDKLVALITRYLGSIK
                     HSDSPLAAGKPLTRATDNASVTVKEQ"
BASE COUNT          272 a          223 c          214 g          200 t
ORIGIN      
        1 gaaccaggac gcattttggg agcaaatggt aaacaatgag cttgccgcca aaaaagcatt
       61 gtctcctgcg gctatcctgg cgctggaaaa agagtacgcc aacaaaaagc tggcggctta
      121 catcttccca ggcagaaatt tatcgttaac agtagacgct gacccacagg cggaaattag
      181 cagcaaagaa acgctggctg agaatctgac atcattaaca ctttccaatg gtgccaaggt
      241 tattctggca aaatccgcgg gtgaagagca aaagctacaa attattgccg tatcgaataa
      301 aggcgattta agtttccctg cgcagcaaaa atcacttatc gcgctggcaa ataaagcagt
      361 tagcggaagc ggcgttggcg aactctcctc ttccagcctg aaacgctgga gtgcggaaaa
      421 ctcggtaacc atgagcagta aagtcagtgg catgaatacg ttgctctccg ttagcgcgcg
      481 gactaataac cctgaacctg gtttccagtt gattaaccag cgaatcaccc acagcacgat
      541 taacgacaat atttgggcat cgctacaaaa tgctcaaatt caggcgttga aaacgctcga
      601 ccagcgtcca gcggagaaat tcgcccagct gatgtatgag acgcgctatg ctgatgaccg
      661 cacgaaatta ctgcaagaaa atcagattgc acagtttact gccgcagatg cgctggctgc
      721 cgatcgccag ttgttttcat ctccagcgga tatcacgttt gtcattgtcg gtaatgtcgc
      781 agaagacaaa ctcgtggcgt taattacgcg ttacttaggc tcaatcaaac actctgattc
      841 gccattagcc gcaggtaaac cattaactcg cgcgacggac aacgcatcgg ttactgtaaa
      901 agaacaaaa
//
LOCUS       DAJTRC010000295          898 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.309, whole genome shotgun sequence.
ACCESSION   DAJTRC010000295 DAJTRC010000000
VERSION     DAJTRC010000295.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 898)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..898
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.309"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            116..>898
                     /gene="curA"
                     /locus_tag="OO357_004267"
     CDS             116..>898
                     /gene="curA"
                     /locus_tag="OO357_004267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415966.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent curcumin/dihydrocurcumin
                     reductase"
                     /protein_id="HCR5888950.1"
                     /translation="MGQQKQRNRRWVLASRPHGAPVPENFRLEEDDVATPGEGQVLLR
                     TVYLSLDPYMRGRMSDEPSYSPPVDIGGVMVGGTVSRVVESNHPDYQPGDWVLGYSGW
                     QDYDISSGDDLVKLGDHPQNPSWSLGVLGMPGFTAYMGLLDIGQPKEGETLVVAAATE
                     PVGATVGQIGKLKGCRVVGVAGGAEKCRHATEVLGFDVCLDHHADDFAEQLAKACPKG
                     IDIYYENVGGKVFDAVLPLLNTSARIPVCGLVSSYNATELPPG"
BASE COUNT          206 a          199 c          281 g          212 t
ORIGIN      
        1 gattcctcga tgaaattatt gcgtgttgca gaggggatgt gacggctgca aacaaaacca
       61 atcccgtgag cctgctacta ttgatggatt catgccaaaa gcacaggaga gcattatggg
      121 gcaacaaaag cagcgtaatc gacgttgggt tctggcctcg cgtccacatg gcgcaccggt
      181 tccggagaat ttccgtcttg aagaagatga tgtcgccaca ccgggtgaag gacaggtgtt
      241 actgcgcaca gtttatttgt ccctggaccc gtatatgcgt ggacgtatga gcgatgagcc
      301 atcttattca ccgccggttg atattggcgg cgtgatggtc ggcggtacgg tgagccgtgt
      361 cgtggagtcg aatcatcctg attatcagcc tggcgactgg gtgctgggct acagtggatg
      421 gcaggactat gacatatcca gtggtgatga cctggtgaaa cttggcgatc atccgcaaaa
      481 tccatcgtgg tcgctgggtg tgctggggat gccaggcttt accgcttata tggggctgct
      541 ggatatcggt cagcctaaag agggcgaaac gttggtggta gctgcggcga cagaaccagt
      601 gggggcgacg gtggggcaaa tcggcaaact taaaggttgc agagtggtgg gggtagccgg
      661 tggcgcggaa aaatgccgcc atgctaccga ggtgttaggc ttcgatgttt gccttgatca
      721 ccacgcggac gattttgccg aacaactggc gaaagcgtgc ccaaaaggta ttgatatcta
      781 ttatgaaaac gtgggcggta aggtatttga tgcagtgcta ccattactta atacatctgc
      841 gcgcattccc gtttgtggct tagtgagcag ctataacgct acagagctac cacccggt
//
LOCUS       DAJTRC010000296          882 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.310, whole genome shotgun sequence.
ACCESSION   DAJTRC010000296 DAJTRC010000000
VERSION     DAJTRC010000296.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 882)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..882
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.310"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..311)
                     /locus_tag="OO357_004268"
     CDS             complement(<1..311)
                     /locus_tag="OO357_004268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5888951.1"
                     /translation="MNSQTTKDIPCFRSYLPDALRLRFEDKLTIRAIAQRLGLSHSTI
                     HTLFQRFLASGIAWPLPDSVSFAQLDAILYANRKKELTEPQIREGSWRKERRTSYSRE
                     F"
BASE COUNT          269 a          172 c          209 g          232 t
ORIGIN      
        1 ttaaattcac ggctatagct ggttcgccgt tcttttcgcc atgagccttc tctgatttga
       61 ggctctgtta attccttctt tctgttggca taaaggatgg cgtcaagttg agcgaatgaa
      121 actgaatcgg gcaatggcca tgcgataccg gatgcaagaa atcgctgaaa aagcgtatgt
      181 attgtggaat gactgagacc cagacgctga gcgatggccc ggatggtcag tttatcttca
      241 aatcttaaac gcagggcatc aggcaaataa gaacggaagc agggaatatc ttttgttgtc
      301 tgggaattca tcgttcgtgt ccatcaatat agatgggcgc gattgttgcc agacaggaca
      361 attttcacaa gacgtcgctg atggggcgct tacattaatc ccgtactctc tggccagttg
      421 ggccacgcag gcccctagct ggtaagattt ttcaataaga tcaagcttaa actccataga
      481 gtcatagagt aagaggcacg aatcctgcgg gggcgaagag aatctgacat tgatagagtc
      541 cattaacgtt ataagaggac tctatcatcg ccaatcatca gacattgaaa tagacgacac
      601 tgagatgacg cttacaattc ttttggtttc tgtaataagt aatctgacat cccccgtgcc
      661 cagaagagga gagggatatc aagcccaagg gttaataatt gatactacgg ttaaaattgc
      721 cttcagtaaa ggaaacgtga ttaaattcag gggatttcag agtgaaaacc atacaacata
      781 ttaaaatctg ttcatttagc tataatacat acaagaaagt gtccagttag ataatgttat
      841 ctaaataacc acagataaaa acgcacataa ttgggggggg gg
//
LOCUS       DAJTRC010000297          858 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.311, whole genome shotgun sequence.
ACCESSION   DAJTRC010000297 DAJTRC010000000
VERSION     DAJTRC010000297.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.311"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(264..>858)
                     /locus_tag="OO357_004269"
     CDS             complement(264..>858)
                     /locus_tag="OO357_004269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001395480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HCR5888952.1"
                     /translation="APTLRIRDPHKDEKMAAIHKALDECSAEHPVFYEDEVDIHLHPK
                     IGADWQLRGQQKRVVTPGQNEKYYLAGALHSGTGKVSYVGGNSKSSALFISLLKRLKA
                     TYRRAKTITLIVDNYIIHKSRETQSWLKENPKFRGIYQPVYSPWVNHVERLWQALHDT
                     ITRNHQCRSMWQLLKKVRHFMETVSPFPGGKHGLAKV"
BASE COUNT          200 a          212 c          206 g          240 t
ORIGIN      
        1 aacaactacg gccatgtcga taaacttcat cggaattaca gcgcggacaa tgaacagtaa
       61 cggtagtcat ggaaaacctc aaataccagt agattaccag aaacttctat cgattgaaga
      121 cattaccttt tgccgcaata ggtggggtaa cattggtgaa aacgcattac gccgggtggc
      181 agttggaaaa aaattatctt ttttcggctc tgatcgtggt ggagaaaatg cggcaataat
      241 ctaaatagct gcgcttaata ccgctacact tttgccagcc catgtttgcc tccggggaat
      301 gggctgacgg tttccataaa atggcgaact tttttcaaca gttgccacat tgagcggcac
      361 tgatgattac gcgttattgt gtcgtgaagt gcctgccata gccgttcaac atgattcacc
      421 catggcgagt aaaccggctg ataaatgccc ctgaacttcg ggttctcctt cagccagctc
      481 tgtgtttccc ggcttttgtg gataatgtag ttgtccacga tcagcgtgat ggttttcgca
      541 cgacggtatg tcgctttaag ccgcttcagc aggctgatga acagcgccga acttttgctg
      601 ttgccgccca catagctgac tttacctgtc ccgctgtgca gcgctccggc cagataatat
      661 ttttcattct gtcccggcgt gaccacccgt ttttgctgtc cgcgcagttg ccagtccgca
      721 ccgattttgg gatgaagatg gatatccact tcatcttcat aaaagaccgg atgctctgcg
      781 ctgcattcgt ccagtgcttt atggattgct gccatctttt catctttatg cgggtcacgg
      841 atacgcagag ttggcgca
//
LOCUS       DAJTRC010000298          840 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.312, whole genome shotgun sequence.
ACCESSION   DAJTRC010000298 DAJTRC010000000
VERSION     DAJTRC010000298.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..840
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.312"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..109
                     /locus_tag="OO357_004270"
     CDS             <1..109
                     /locus_tag="OO357_004270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="T3SS effector OspC family protein"
                     /protein_id="HCR5888953.1"
                     /translation="NSGDTMLDNAMKSKDSKMIDFLLKNGAILGKRFEI"
     gene            447..840
                     /locus_tag="OO357_004271"
                     /pseudo
     CDS             447..840
                     /locus_tag="OO357_004271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611998.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          252 a          177 c          204 g          207 t
ORIGIN      
        1 aaatagtggg gacactatgc ttgacaatgc aatgaaaagc aaagattcaa aaatgattga
       61 ttttttatta aaaaatggag caatattagg caaacgattt gagatatgac ctaaaagctc
      121 ggccaaataa taatgtcgtc gattatcgaa gtcatcatga gtgattctgt ccccacagtg
      181 cagcatcagc atgacagtga attgtctggc gtggtccatt taatgagatg gagatggtcc
      241 ctctttccta aatagctgtg cctaataccg ctacactttt gccagcccat gtttgcctcc
      301 ggggaatggg ctgacggttc cttcaaaatc cattgcacgc agttatgtcg gagcgggaac
      361 ttgactcctg tctttacggg agtgaggtag cctgagttta acggacactc cttcctgaaa
      421 tagaatggca tcagaaggag ccaataatga gcagaaaaac ccaacgttac tctacggagt
      481 ttaaagccga agccgtcaaa acggttcctg aaaatcagct ttcgatcagt gaaggagctt
      541 cccgattatc tgttcctgaa ggcactttag gacaatgggt taccgccgca agaaaagggc
      601 tcaatacacc aggctcccgc acagtggctg agctggaatc tgaagttatg caactgcgta
      661 aggctttaaa tgaagcacga cttgagcgag atatattaaa aaaagcaaca gcgtattttg
      721 cacaggagtc gctgaaaaat acgcgttagt cgaacaatgg cgacaacaat tccctgttga
      781 agcgatgtgt cgggtacctg gtgtatccag gagcggttat tacgaccggg tacagcatgc
//
LOCUS       DAJTRC010000299          834 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.313, whole genome shotgun sequence.
ACCESSION   DAJTRC010000299 DAJTRC010000000
VERSION     DAJTRC010000299.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 834)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 834)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..834
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.313"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..375
                     /locus_tag="OO357_004272"
     CDS             <1..375
                     /locus_tag="OO357_004272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000902876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber assembly protein"
                     /protein_id="HCR5888954.1"
                     /translation="RVVWVGKDVTGIEPRNASVIEVPDITANRRITAPGYWFYRNDEF
                     VFDYRLKAEDERDALLAQVSARTGEWEEDLLLGLISDEDKEKLKACRIYAKSLQAMDF
                     STITDKSSYNAIEWPASPEGSS"
     gene            399..>834
                     /locus_tag="OO357_004273"
     CDS             399..>834
                     /locus_tag="OO357_004273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prophage tail fiber N-terminal domain-containing
                     protein"
                     /protein_id="HCR5888955.1"
                     /translation="MSVVISGALTDGAGIPMSGYHIILKSRVNPPEVVMNTVADVMTG
                     NDGEYCFHARTGKYGVYLKQDWRNEYNVGDIAVYEDSKPGTLNDFLIAPDEGDLKPDV
                     VKRFEEMVAQAQQSAGAAAGNAQQTAQDVAAAAGYARAAEQAK"
BASE COUNT          214 a          197 c          243 g          180 t
ORIGIN      
        1 cgcgtggtgt gggtcggtaa ggatgtgaca ggcattgaac cccgtaacgc cagtgttatt
       61 gaagttcctg atattaccgc caaccgccgc attaccgcgc cgggttactg gttttaccgc
      121 aatgatgaat ttgtctttga ctacagactc aaagcggaag atgagcgtga tgcccttctg
      181 gctcaggtca gtgcccggac aggggaatgg gaagaagacc tgctgctggg gttaatcagc
      241 gacgaagata aagaaaagct gaaagcctgt cgtatttacg cgaaatcgct gcaggcgatg
      301 gatttcagca ccatcactga taaatcctca tacaacgcca ttgaatggcc cgcctctccg
      361 gaaggttctt cctgatttaa tttatcgcga gaaaaacaat gtctgtagtg atatcaggtg
      421 cgctgactga tggcgcaggt atccccatgt ccggatacca tattattctg aaatcccggg
      481 taaacccccc ggaagtggtg atgaacactg ttgctgatgt gatgacagga aacgatggtg
      541 aatactgttt ccatgcgcgg actggaaaat atggtgtgta tctgaaacag gactggcgca
      601 acgagtacaa cgttggcgac attgctgtat atgaggactc aaagcccggc acgctgaatg
      661 actttctgat tgctcctgat gagggcgacc tgaaaccgga tgtcgtcaaa cgctttgagg
      721 aaatggtggc gcaggcgcag cagagcgccg gggccgcagc cggaaacgca cagcagacgg
      781 cgcaggatgt ggcggcagcc gcaggttatg cccgcgcagc agagcaggcc aaaa
//
LOCUS       DAJTRC010000300          806 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.314, whole genome shotgun sequence.
ACCESSION   DAJTRC010000300 DAJTRC010000000
VERSION     DAJTRC010000300.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.314"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            306..667
                     /locus_tag="OO357_004274"
                     /pseudo
     CDS             306..667
                     /locus_tag="OO357_004274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477181.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS1 family transposase"
BASE COUNT          223 a          183 c          178 g          222 t
ORIGIN      
        1 acgccctgtg gttgaaacgt gagcatattg ttttcagatg ctgttgtctt gcttcagagt
       61 gatctgtcct ccggatttac gcaaagttat ccacaggcac tgtgcattta aaaaacttat
      121 cttttcttct tgattttaaa aactcagatc gtcatatcat tatgataaac ataataaaaa
      181 actctaaaca tgagaaggtt tttaacgtaa ggtgagagga tttttaagtg tatccataga
      241 ttaaaaatga caatgcctcc aattagttga attaggggtt ataatacccg cttttaggtt
      301 ctttcatggt cacagttaac tttcattgcc cccgttgcca gtccgttcag gtttaccgtc
      361 acgggcagaa cccaaaagga catgatcgat tccgttgccg tgactgtcac cgggtgtttc
      421 agctcactta ctgttacgag gcccgtaaac cgggagttaa aaaccaaatc actgaaatgg
      481 ctttcaacgg tgccggagtt cgcgatactg gccgtacgct gaaaatcggc atcaataccg
      541 tcatacggac attaaaaagc tcccgccccg gcggataaca tctgcgccag tcgctcacgc
      601 tgatgttgcc cttatctgtg aacttgatgg gcagtggagc ttcgttggca gcaaggcgca
      661 acaacactgt cacgaacggt gcaatagtga tccacaccca acgcctgaaa tcagatccag
      721 ggggtaatct gctctcctga ttcaggagag cttatggtca cttttgagac agttatggaa
      781 attaaaatcc tgcacaagca gggaat
//
LOCUS       DAJTRC010000301          799 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.315, whole genome shotgun sequence.
ACCESSION   DAJTRC010000301 DAJTRC010000000
VERSION     DAJTRC010000301.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 799)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..799
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.315"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            104..>799
                     /locus_tag="OO357_004275"
     CDS             104..>799
                     /locus_tag="OO357_004275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5888956.1"
                     /translation="MIKSTNIQVIGSGIMHQINNIHSLTLFSLPVSLSPSCNEYYLKV
                     WSEWEKNGTPGEQRNIAFNRLKICLQNQGAELNLSELDLKTLPDLPPQITTLEIRKNL
                     LTYLPDFPPMLKVIHAQFNQLESLPALPETLEELNVGDNKIKELPYLPETLTHLRIHN
                     NRLHILPLLPPELKLLIVSGNRLDSIPPFPDKLEGLALANNFIEQLPELPFSMNRAVL
                     MNNNLTTLPESVLR"
BASE COUNT          279 a          152 c          138 g          230 t
ORIGIN      
        1 aagcaatacc tggagaaaga gtacaaagca aactgtatta aacttaataa gttatattgt
       61 atttcactag agttcttatc gttgataaga aattctggtt ataatgatta aatcaacgaa
      121 tatacaggta atcggttctg gtattatgca tcaaataaac aacatacact cgttaactct
      181 attttctttg cctgtgagtt tgagtccatc ttgtaatgaa tattatttaa aagtctggag
      241 tgaatgggaa aagaacggta ccccaggcga gcaacgcaat atcgccttca ataggctaaa
      301 aatatgtttg caaaatcaag gggcggaatt aaatctatct gagttagatt taaaaacatt
      361 acctgattta ccgcctcaga taacaacact ggaaataaga aaaaacctat taacatatct
      421 ccctgatttc ccaccaatgc ttaaggtaat acatgctcaa tttaatcaac tggaaagctt
      481 acctgcctta cccgagacgt tagaagagct taatgtgggt gataacaaga taaaagaatt
      541 accatatctt cctgaaactc taactcattt acggatccat aataaccgat tgcatattct
      601 gccactattg ccaccggagc taaaattact gatagtttct ggaaacagat tagacagcat
      661 cccccccttt ccagataagc ttgaagggct ggctctggct aataatttta tagaacaact
      721 accggaatta ccttttagta tgaacagggc tgtgctaatg aataataatc tgacaacact
      781 tccggaaagt gtcctgaga
//
LOCUS       DAJTRC010000302          778 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.316, whole genome shotgun sequence.
ACCESSION   DAJTRC010000302 DAJTRC010000000
VERSION     DAJTRC010000302.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.316"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(32..697)
                     /locus_tag="OO357_004276"
     CDS             complement(32..697)
                     /locus_tag="OO357_004276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HCR5888957.1"
                     /translation="MTVADFKRPKLELPNGANKLLLHSCCAPCSGEVMEALQASEIDY
                     TIFFYNPNIHPQKEYLIRKDENIRFAEQHGVPFIDADYDTDNWFERAKGMEWEPERGI
                     RCTMCFDMRFERTALYAAENGFSVISSSLGISRWKNMQQVNDCGRRAVAHYPGMVYWD
                     YNWRKQGGSSRMIEISKREKFYQQEYCGCVYSLRDTNLHRKSQGRPLIKIGQLHYGKE
                     EKE"
BASE COUNT          204 a          190 c          178 g          206 t
ORIGIN      
        1 atgaaatcaa tcagaaaggt gccccataaa atcactcctt ctcttcttta ccgtagtgga
       61 gttgaccaat tttgataaga gggcgtccct gagatttgcg gtgtagattg gtatcgcgca
      121 gagaatacac acagccacaa tattcctgct gatagaattt ttcgcgcttg ctgatttcaa
      181 tcatacggga cgagccgccc tgcttgcgcc agttataatc ccagtacacc atgcccggat
      241 aatgcgcgac ggctcgccgc ccacagtcgt taacctgctg catatttttc cagcgtgaaa
      301 tgcccagtga actgctgatc acactgaaac cattttcagc ggcgtacagc gctgtccgct
      361 caaaacgcat gtcaaaacac atggtgcaac ggatccctcg ttcgggctcc cattccattc
      421 ctttggctcg ttcaaaccag ttgtctgtgt cgtaatcagc atcaataaac ggcacgccgt
      481 gttgttcagc aaagcgaata ttctcatcct tacgaattaa atactctttc tgaggatgaa
      541 tgttcgggtt gtagaaaaag atggtgtagt cgatttccga ggcctgaagc gcctccatca
      601 cctcaccgga acatggagca cagcaagagt gcagtagtag tttgtttgcc ccgtttggga
      661 gctccaattt gggccgtttg aaatcagcaa ctgtcataaa tatgtttatt gaggtcataa
      721 aaatatcaaa gagtgtagca ttagaacggg gctatcggaa acagatgtgt gactgctc
//
LOCUS       DAJTRC010000303          778 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.317, whole genome shotgun sequence.
ACCESSION   DAJTRC010000303 DAJTRC010000000
VERSION     DAJTRC010000303.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..778
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.317"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(22..594)
                     /locus_tag="OO357_004277"
     CDS             complement(22..594)
                     /locus_tag="OO357_004277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaY family lipoprotein"
                     /protein_id="HCR5888958.1"
                     /translation="MKLVHMASGLAVAIALAACADKSADIQTPAPAANTSISATQQPA
                     IQQPNVSGTVWIRQKVALPPDAVLTVTLSDASLADAPSKVLAQKAVRTEGKQSPFSFV
                     LPFNPADVQPNARILLSAAITVNDKLVFITDTVQPVINQGGTKADLTLVPVQQTAVPV
                     QASGGATTTVPSTSPTQVNPSSAVPAPTQY"
BASE COUNT          182 a          200 c          206 g          190 t
ORIGIN      
        1 ggcggagagg gttaacgtcg cttaatattg cgtaggagcg ggaactgccg aagacggatt
       61 cacctgagtt ggtgaagtcg aaggtacggt agtcgttgcg ccaccgctgg cctgaaccgg
      121 cacggcggtt tgctgtaccg gcaccaatgt caggtcggct ttagttccgc cctggttgat
      181 caccggctga acggtatcgg tgataaatac cagtttgtca ttcacggtaa tcgccgcact
      241 caacagaata cgcgcgttcg gctgaacatc tgccgggtta aatggcagaa caaagctgaa
      301 tggtgactgt ttaccttcag tacgcaccgc tttctgcgcc agcacttttg acggtgcatc
      361 ggctaacgac gcgtcagaaa gtgtcacggt cagcacagca tcaggcggca gtgcgacttt
      421 ctgacggatc cagacagtac cggagacatt cggttgctgg atagcaggtt gttgtgttgc
      481 tgaaatagac gtatttgcag ccggggctgg cgtctgaata tccgcgcttt tatctgcgca
      541 agccgccaac gcaatcgcaa ccgctaaacc actggccatg tgcacgagtt tcattcattt
      601 ctccttatta tcaatgcacc agcgggctaa ctttcctcgc cggaagagtg gttaacaaaa
      661 tagtaacgtc aacaagtgtg gcacacatca cgcatttctg cctgtaatta gcccgtaatt
      721 cagactattt cacccatcgg accagtggca aatttgcgtt atactcaact cactttgg
//
LOCUS       DAJTRC010000304          740 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.318, whole genome shotgun sequence.
ACCESSION   DAJTRC010000304 DAJTRC010000000
VERSION     DAJTRC010000304.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..740
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.318"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..>740)
                     /locus_tag="OO357_004278"
     CDS             complement(<1..>740)
                     /locus_tag="OO357_004278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5888959.1"
                     /translation="PIGLMDTVATVTPAQLRQFYQRWYQPNNMTFIVVGDIDSKEALA
                     LIKDNLSKLPANKAAENRVWPTKAENHLRFNIINDKENRVNGIALYYRLPMVQVNDEQ
                     SFVEQAEWSMLVQLFNQRLQERIQSGELKTISGGTARSVKIAPDYQSLFFRVNARDDN
                     MQDAANALMAELATIDQHGFSAEELDDVKSTRLTWLKNAVDQQAERDLRMLTSRLASS
                     SLNNTPFLSPEETYQLSKRLWQQITVQSL"
BASE COUNT          180 a          175 c          173 g          212 t
ORIGIN      
        1 agactttgca cggtaatttg ctgccacaga cgtttcgaaa gttgatatgt ctcttccggc
       61 gacaagaacg gcgtattatt taatgagctg gatgccaggc gactggtcag catacgtaaa
      121 tcacgttcag cttgctgatc aaccgcattt ttcagccagg tgaggcgggt agatttgaca
      181 tcatcgagtt cttcagcaga aaaaccatgc tgatcaatgg ttgccaactc tgccattaat
      241 gcattcgcag catcctgcat attatcgtct cgtgcattta cacggaaaaa cagcgactga
      301 taatcgggtg caattttaac gctgcgcgca gtgccgccag aaatagtctt caactcgccc
      361 gactgtatgc gttcctgcag acgttgattg aacagctgaa ctaacatgct ccattcagct
      421 tgttcgacaa agctttgctc atcgttcact tgtaccattg gcaggcgata atagagtgcg
      481 atgccgttca cccggttttc tttatcatta atgatattaa agcgcaggtg gttttcggct
      541 tttgtcggcc agacgcgatt ttcagctgct ttgttagccg gaagcttact taaattatcc
      601 tttatcagcg ccagcgcttc tttactgtcg atatcgccga ccacgataaa ggtcatatta
      661 tttggttgat accagcgttg ataaaattgg cgcaattgtg ccggcgtgac cgtggcgaca
      721 gtatccatca ggccgatagg
//
LOCUS       DAJTRC010000305          739 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.319, whole genome shotgun sequence.
ACCESSION   DAJTRC010000305 DAJTRC010000000
VERSION     DAJTRC010000305.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 739)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 739)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..739
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.319"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            1..739
                     /locus_tag="OO357_004279"
                     /pseudo
     CDS             1..739
                     /locus_tag="OO357_004279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012477168.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3-like element IS3 family transposase"
BASE COUNT          176 a          202 c          210 g          151 t
ORIGIN      
        1 attcgcctga atttcgcagt gaagccctga agcttgctga acgcatcggt gttactgccg
       61 cagcccgtga actcagcctg tatgaatcac agctctacaa ctggcgcagt aaacagcaaa
      121 atcagcagac gtcttctgaa cgtgaactgg agatgtctac cgagattgca cgtctcaaac
      181 gccagctggc agaacgggat gaagagctgg ctatcctcca aaaggccgcg acatacttcg
      241 cgaagcgcct gaaatgaagt atgtctttat tgaaaaacat caggctgagt tcagcatcaa
      301 agcaatgtgc cgcgtgctcc gggtggcccg cagcggctgg tatacgtggt gtcagcggcg
      361 gacaaggata agcacgcgtc agcagttccg ccaacactgc gacagcgttg tcctcgcggc
      421 ttttacccgg tcaaaacagc gttacggtgc cccacgcctg acggatgaac tgcgtgctca
      481 gggttacccc tttaacgtaa aaaccgtggc ggcaagcctg cgccgtcagg gactgagggc
      541 aaaggcctcc cggaagttca gcccggtcag ctaccgcgca cacggcctgc ctgtgtcaga
      601 aaatctgttg gagcaggatt tttacgccag tggcccgaac cagaagtggc caggagacat
      661 cacgtactta cgtacagatg aaggctggct gtatctggca gtggtcattg acctgtggtc
      721 acgtgccgtt attggctgg
//
LOCUS       DAJTRC010000306          733 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.320, whole genome shotgun sequence.
ACCESSION   DAJTRC010000306 DAJTRC010000000
VERSION     DAJTRC010000306.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 733)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 733)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..733
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.320"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            114..353
                     /gene="relB"
                     /locus_tag="OO357_004280"
     CDS             114..353
                     /gene="relB"
                     /locus_tag="OO357_004280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin RelB"
                     /protein_id="HCR5888960.1"
                     /translation="MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERL
                     PFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL"
     gene            353..640
                     /gene="relE"
                     /locus_tag="OO357_004281"
     CDS             353..640
                     /gene="relE"
                     /locus_tag="OO357_004281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system mRNA interferase
                     RelE"
                     /protein_id="HCR5888961.1"
                     /translation="MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKL
                     RGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGKRERSEVYSEAVKRIL"
BASE COUNT          197 a          150 c          192 g          194 t
ORIGIN      
        1 cggacagtga tcaccgttct tacgactact ttctgacttc cttcgtgact tgccctaagc
       61 atgttgtagt gcgatacttg taatgacatt tgtaattaca agaggtgtaa gacatgggta
      121 gcattaacct gcgtattgac gatgaactta aagcgcgttc ttacgccgcg cttgaaaaaa
      181 tgggtgtaac tccttctgaa gcgcttcgtc tcatgctcga gtatatcgct gacaatgaac
      241 gcttgccgtt caaacagaca ctcctgagtg atgaagatgc tgaacttgtg gagatagtga
      301 aagaacggct tcgtaatcct aagccagtac gtgtgacgct ggatgaactc tgatggcgta
      361 ttttctggat tttgacgagc gggcactaaa ggaatggcga aagctgggct cgacggtacg
      421 tgaacagttg aaaaagaagc tggttgaagt acttgagtca ccccggattg aagcaaacaa
      481 gctccgtggt atgcctgatt gttacaagat taagctccgg tcttcaggct atcgccttgt
      541 ataccaggtt atagacgaga aagttgtcgt tttcgtgatt tctgttggga aaagagaacg
      601 ctcggaagta tatagcgagg cggtcaaacg cattctctga accaaagcat gacatctctg
      661 tttcgcaccg aaggtgacac ttctgctttg cgttgacagg agaagcaggc tatgaagcag
      721 caaaaggcga tgt
//
LOCUS       DAJTRC010000307          731 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.321, whole genome shotgun sequence.
ACCESSION   DAJTRC010000307 DAJTRC010000000
VERSION     DAJTRC010000307.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 731)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 731)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..731
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.321"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..>731
                     /locus_tag="OO357_004282"
     CDS             <1..>731
                     /locus_tag="OO357_004282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66-like element ISSfl3 family transposase"
                     /protein_id="HCR5888962.1"
                     /translation="YASAGLLARILVSKYMEHIPLYRQSEIYARQGVELSRNTMVRWV
                     SEMADKLRPLYIALNDYVLEAGKVHADDTPVKVLAPGNGKTKTGRLWVYVRDDRNAGS
                     SLPAAVWFAYSADRKGEHPQLHLAKYQGVLQADAYAGYNVLYETGRVKEAGCLAHARR
                     KIHDEDVRRPTEMTQEALRRIAELYDIEAEIRGSPAEERLAVRKARSVQLMQSLYDWI
                     QLQRKTLSKHAEMAKAFDYILNHWNA"
BASE COUNT          196 a          171 c          224 g          140 t
ORIGIN      
        1 tatgccagtg cagggttact tgcacggatc ctggtcagca aatatatgga acatatccct
       61 ttatatcgcc agtcagaaat atacgcgcga cagggcgtgg agctgagccg taataccatg
      121 gtgcgctggg tatcagaaat ggcagacaaa ctccgtcctc tgtatatagc gctgaatgac
      181 tatgttctgg aggcaggaaa ggtgcacgca gatgacactc cggtgaaagt actggccccg
      241 gggaacggaa agacgaaaac gggtcgtctg tgggtatacg tcagggatga tcgtaatgcg
      301 ggttcatccc tgccggcagc cgtctggttc gcgtattcgg cagatcgcaa aggagaacat
      361 ccgcagctcc acctggcaaa gtatcagggc gtactgcagg ctgatgccta tgcaggttat
      421 aacgtactgt acgaaacggg ccgggtgaag gaagccgggt gcctggccca cgcccgccga
      481 aaaatccatg acgaggatgt gcgccgtccg acagaaatga ctcaggaagc gctcagacgg
      541 atagcagagt tatacgacat agaagcggag atacgtggca gtccggcaga ggaacggctt
      601 gcagtcagaa aagccagaag cgtccagttg atgcagtcgt tgtacgactg gatacagttg
      661 cagaggaaaa cgctgtcgaa acatgcggag atggcgaagg cgttcgacta tatcctgaat
      721 cactggaatg c
//
LOCUS       DAJTRC010000308          727 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.322, whole genome shotgun sequence.
ACCESSION   DAJTRC010000308 DAJTRC010000000
VERSION     DAJTRC010000308.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 727)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..727
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.322"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..452)
                     /locus_tag="OO357_004283"
     CDS             complement(<1..452)
                     /locus_tag="OO357_004283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5888963.1"
                     /translation="MKRNIIGGAFTLASLMLAGHALAEDGVVNFVGEIVDTTCEVTSD
                     TADQIVPLGKVSKNAFSGVGSLASPQKFSIKLENCPATYTQAAVRFDGTEAPGGDGDL
                     KVGTPLTAGNPGDFTGTGQAIAATGVGIRIFNQSDNSQVKLYNDSAYTA"
BASE COUNT          234 a          146 c          139 g          208 t
ORIGIN      
        1 gcggtataag cagagtcgtt ataaagttta acctgcgaat tatcggactg gttaaaaata
       61 cgaataccaa cgccggttgc cgcaatcgct tgtcctgtac cggtaaaatc accagggttg
      121 cctgctgtaa gcggcgtacc cactttcagg tcgccgtcgc cgccaggcgc ttctgtacca
      181 tcaaaacgaa cggctgcttg agtgtacgtt gccgggcaat tttcaagttt aatactgaac
      241 ttctgtggcg acgccagact acctacacct gaaaatgcat ttttactgac tttacccagt
      301 gggacaattt gatcggctgt atcggaggta acttcacaag tagtgtcgac aatttcaccg
      361 acgaagttaa caacaccatc ttctgccagt gcatgcccgg ccagcattag agatgccaga
      421 gtgaatgcac cgcctataat attacgcttc ataaaaactc cattgatata atatatcctt
      481 taccatgaat catcacttat attaataagt gtgtttttga ttcaaaggaa tagtacacca
      541 aagagacggc tttatctaaa aataatatta attacgcaaa aataaaatat agatttattg
      601 tttaaatatt atttaaacag taaaaaatca agtttgttat cagacattaa tgataattca
      661 gaatatattt atatagaaag atagatgaat ttattgatgt cagttataaa aaagggccac
      721 cggtcgg
//
LOCUS       DAJTRC010000309          721 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.323, whole genome shotgun sequence.
ACCESSION   DAJTRC010000309 DAJTRC010000000
VERSION     DAJTRC010000309.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..721
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.323"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..>721
                     /locus_tag="OO357_004284"
     CDS             <1..>721
                     /locus_tag="OO357_004284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707663.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3418 domain-containing protein"
                     /protein_id="HCR5888964.1"
                     /translation="DGEDWHWDQVPDHLKITFRVVDDKNKKLKEGRSLQDLKDALKGK
                     VQETLSAVADDGIEQSGLHIWSFGQLPESYEQKRGNYKVKAWPALVDERDSVTIKLFD
                     NPLEQKQAMWNGLRRLLLLNISSPIKYLHEKLPNKAKLGLYFNPYGKVLELIDDCISC
                     GVDQLIDANGGPVWTEEGFAALHEKVRAELNDTVVDIAKQVEQILTAVFNINKRLKGR
                     VDMTMALGLSDIKAQMGGLVYR"
BASE COUNT          196 a          157 c          220 g          148 t
ORIGIN      
        1 agacggcgaa gactggcact gggatcaggt gcccgatcac ctgaaaatca ccttccgcgt
       61 ggtggatgac aaaaacaaga agctaaaaga agggcgctcg ctacaagatc tgaaagatgc
      121 gctgaaaggc aaagtgcagg aaacgctgtc cgcggtggcg gatgacggta tcgagcagag
      181 cggcttacat atctggagtt ttggtcagct gccggaaagt tacgaacaga agcgtggcaa
      241 ctataaagtg aaggcgtggc cggcgctggt ggatgaacgc gacagcgtga cgatcaaact
      301 gtttgataac ccgctggagc aaaaacaggc aatgtggaat ggtcttcgcc gtctactgct
      361 gctaaatatt tcctcgccga tcaagtattt acatgagaag ttaccaaaca aagccaagct
      421 ggggctgtac tttaacccgt atggcaaagt gctggagctg atcgacgact gtatctcctg
      481 cggtgtggat caactgatcg acgccaatgg tggcccggtc tggacggaag aaggctttgc
      541 tgcgctgcat gaaaaagtgc gcgccgaact gaatgacacg gtggtggata ttgcgaagca
      601 ggtcgagcaa atcctcactg cggtgttcaa tatcaacaaa cgtctgaaag ggcgggtgga
      661 tatgaccatg gcactggggc tttctgatat taaagcgcag atgggcgggt tggtatatcg
      721 c
//
LOCUS       DAJTRC010000310          703 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.324, whole genome shotgun sequence.
ACCESSION   DAJTRC010000310 DAJTRC010000000
VERSION     DAJTRC010000310.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 703)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..703
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.324"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..>703
                     /locus_tag="OO357_004285"
     CDS             <1..>703
                     /locus_tag="OO357_004285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HCR5888965.1"
                     /translation="NDPVPAGIACIAIVWVFTFVNMLGGTWVSRLTTIGLVLVLIPVV
                     MTAIVGWHWFDAATYAANWNTADTTDGHAIIKSILLCLWAFVGVESAAVSTGMVKNPK
                     RTVPLATMLGTGLAGIVYIAATQVLSGMYPSSVMAASGAPFAISASTILGNWAAPLVS
                     AFTAFACLTSLGSWMMLVGQAGVRAANDGNFPKVYGEVDSNGIPKKGLLLAAVKMTAL
                     MILITLMNSAGGKAS"
BASE COUNT          136 a          163 c          191 g          213 t
ORIGIN      
        1 taaatgatcc tgttccggcg ggtatcgcct gtattgctat cgtctgggta tttacctttg
       61 taaatatgct cggcggtacc tgggtaagcc gtttaaccac tattggtctg gtgctggttc
      121 ttattcctgt ggtgatgact gctattgttg gctggcattg gtttgatgcg gcaacttatg
      181 cagctaactg gaatactgcg gataccactg atggtcatgc gatcattaaa agtattctgc
      241 tctgcctgtg ggccttcgtg ggtgttgaat ccgcagcagt aagtactggt atggttaaaa
      301 acccgaaacg taccgttccg ctggcaacca tgctgggtac tggtttagca ggtattgttt
      361 atatcgctgc gactcaggtg ctttccggta tgtatccgtc ttctgtaatg gcggcttccg
      421 gtgctccgtt tgcaatcagt gcttcaacta tcctcggtaa ctgggctgcg ccgctggttt
      481 ctgcattcac cgcctttgcg tgcctgactt ctctgggctc ctggatgatg ttggtaggcc
      541 aggcaggtgt acgtgccgct aacgacggta acttcccgaa agtttatggt gaagtcgaca
      601 gcaacggtat tccgaaaaaa ggtctgctgc tggctgcagt gaaaatgact gccctgatga
      661 tcctcatcac tctgatgaac tctgccggtg gtaaagcatc tga
//
LOCUS       DAJTRC010000311          702 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.325, whole genome shotgun sequence.
ACCESSION   DAJTRC010000311 DAJTRC010000000
VERSION     DAJTRC010000311.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.325"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..>702
                     /locus_tag="OO357_004286"
     CDS             <1..>702
                     /locus_tag="OO357_004286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="E3 ubiquitin--protein ligase"
                     /protein_id="HCR5888966.1"
                     /translation="NITDYHRIWDNWAKEGAATEDRTQAVRLLKICLAFQEPALNLSL
                     LRLRSLPYLPPHIQELNISSNELRSLPELPPSLTVLKASDNRLSRLPALPPHLVALDV
                     SLNRVLTCLPSLPSSLQSLSALLNSLETLPDLPPALQKLSVGNNQLTALPELPCELQE
                     LSAFDNRLQELPPLPQNLRLLNVGENQLHRLPELPQRLQSLYIPNNQLNTLPDSIMNL
                     HIYADVNIYNNPLSTR"
BASE COUNT          196 a          204 c          117 g          185 t
ORIGIN      
        1 aatatcacgg attatcaccg catctgggat aactgggcca aggaaggtgc tgcaacagaa
       61 gaccgaacac aggcagttcg attactgaaa atatgtctgg cttttcaaga gccagccctc
      121 aatttaagtt tactcagatt acgctctctc ccatacctgc ccccgcacat acaagaactt
      181 aacatctcta gcaatgagct acgctctctg ccagaactcc ctccgtcctt aactgtactt
      241 aaagccagcg ataacagact gagcaggctc ccggctcttc cgcctcacct ggtcgctctt
      301 gatgtttcac ttaacagagt tttaacatgt ttgccttctc ttccatcttc cttgcagtca
      361 ctctcagccc ttctcaatag cctggagacg ctacctgatc ttcccccggc tctacaaaaa
      421 ctttctgttg gcaacaacca gcttactgcc ttaccagaat taccatgtga actacaggaa
      481 ctaagtgctt ttgataacag attacaagag ctaccgcccc ttcctcaaaa tctgaggctt
      541 ttaaacgttg gggaaaacca actacacaga ctgcccgaac ttccacaacg tctgcaatca
      601 ctatatatcc ctaacaatca gctgaacaca ttgccagaca gtatcatgaa tctgcacatt
      661 tatgcagatg ttaatattta taacaatcca ttgtcgactc gc
//
LOCUS       DAJTRC010000312          668 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.326, whole genome shotgun sequence.
ACCESSION   DAJTRC010000312 DAJTRC010000000
VERSION     DAJTRC010000312.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 668)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..668
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.326"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..>668
                     /locus_tag="OO357_004287"
     CDS             <1..>668
                     /locus_tag="OO357_004287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010921623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110-like element ISSfl4 family transposase"
                     /protein_id="HCR5888967.1"
                     /translation="ARTLPHALRTLKLADEQIAELSMLCGFDDDLAAQTTQASNRIRG
                     LLTQIHPAPERVLGPRLEHPAVLDLLQRYPSPEKLASLGEKKLAAQLCKLAPRLGKRL
                     AADIAQALAEQTVVVPGTNAAAVVLPRLALQLITLRKQRDEVALEVEQRVLAHPLYPV
                     LTSMPGVGVRTAARLLTEVACRAFASAAHLAAYAGLAPVTRRSGSSIRGEHPSRRGNK
                     ALKR"
BASE COUNT          133 a          230 c          177 g          128 t
ORIGIN      
        1 ctgcccgtac cctgcctcac gcgctacgca cgctgaaact ggctgacgag caaatcgccg
       61 aactctccat gctctgcggc ttcgatgatg atcttgccgc acagacaacg caggccagca
      121 accgtatccg cggccttctg acccagatac atccggcacc ggagcgcgtt ctcggtccga
      181 gacttgagca cccggcggta ctcgatcttc tccagcgata tccctcacca gaaaaactcg
      241 cttcgctggg tgagaagaag ctggcagccc agctctgcaa acttgcgcct cgtctgggta
      301 aacgccttgc agcagacata gctcaggcac tggccgaaca aaccgtcgtc gttcccggca
      361 cgaatgccgc tgccgtagta ctgccacgtc tggcactcca gctcatcacg ctgcgtaagc
      421 aaagagacga ggtggcgctt gaggtagaac agcgagttct tgctcaccct ctttacccgg
      481 tcctgaccag tatgcccgga gtcggtgtca ggaccgcagc cagactcctc accgaggtcg
      541 cctgccgcgc cttcgcctct gccgcacatc tcgctgctta tgctggcctt gcgccggtaa
      601 ctcggcgatc cggctcgtca atacgcggtg agcatccctc gcgacggggt aataaagctc
      661 tcaaacgg
//
LOCUS       DAJTRC010000313          653 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.327, whole genome shotgun sequence.
ACCESSION   DAJTRC010000313 DAJTRC010000000
VERSION     DAJTRC010000313.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 653)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 653)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..653
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.327"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..>653)
                     /locus_tag="OO357_004288"
     CDS             complement(<1..>653)
                     /locus_tag="OO357_004288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HCR5888968.1"
                     /translation="PPIQQPYPPLYFGGSSDVAQELAAEQVDLYLTWGEPPELVKEKI
                     EQVRAKAAAYGRKIRFGIRLHVIVRETNDEAWQAAERLISHLDDETIAKAQAAFARTD
                     SVGQQRMAALHNGKRDNLEISPNLWAGVGLVRGGAGTALVGDGPTVAARINEYAALGI
                     DSFVLSGYPHLEEAYRVGELLFPHLDVAIPEIPQPQPLNPQGEAVANDFIPRKVAQS"
BASE COUNT          134 a          194 c          181 g          144 t
ORIGIN      
        1 agctttgcgc gactttacgg gggataaaat catttgccac cgcttcgcct tgcggattca
       61 gcggctgcgg ctggggaatt tccggaatgg cgacatccag atgtgggaac agcaactcgc
      121 caacccgata cgcttcttcc agatgcgggt agcccgaaag cacaaagctg tcgatgccaa
      181 gcgcggcgta ttcgttgatt cgcgcggcga ccgtaggacc atcgcccacc agcgccgttc
      241 cggcaccgcc gcgcactaag ccaacgcccg cccataaatt ggggctgatc tccagattgt
      301 cgcgcttgcc gttatgtagc gccgccattc gctgttgtcc tacggaatcc gtccgggcga
      361 atgcggcttg tgctttggcg atagtctcat catcaagatg cgagattaac cgctcggcgg
      421 cctgccacgc ttcgtcgtta gtttcacgaa caatcacatg cagacgaata ccgaaacgaa
      481 ttttgcgccc atacgcggca gctttcgccc gcacttgttc gattttctct ttaacaagtt
      541 ccggcggttc gccccaggtg aggtagagat caacttgttc tgccgccagc tcctgggcga
      601 catctgacga tccgccaaag taaagcggag gataaggctg ttgaatcggc ggg
//
LOCUS       DAJTRC010000314          652 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.328, whole genome shotgun sequence.
ACCESSION   DAJTRC010000314 DAJTRC010000000
VERSION     DAJTRC010000314.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 652)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 652)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..652
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.328"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..648)
                     /gene="virK"
                     /locus_tag="OO357_004289"
     CDS             complement(<1..648)
                     /gene="virK"
                     /locus_tag="OO357_004289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor VirK"
                     /protein_id="HCR5888969.1"
                     /translation="MFSISNLSFIGFLKRIIFSSDSLPGKWEHRKFRFMYILRCSINP
                     VVSIRYYYELRSLPCIEDILAIHPTLPARIHRPYLHKGGRAWTRGQYILEHYRFVQNL
                     PEKYSKFLFPQKSVSLVQFIGKDGENFDIQCSPSGFDREGELMLSLFYNKTVIARLTF
                     SVILTQNGHIAFIGGLQGAPKNTGPDVIRCATRACYGIFPKRIIFEAFCALMKACN"
BASE COUNT          216 a          137 c          123 g          176 t
ORIGIN      
        1 attgcaagct ttcatcaacg cacaaaatgc ttcaaaaata atccgttttg ggaaaatccc
       61 ataacaggcc cgggttgcgc accgaataac atcaggtccg gtatttttag gggctccctg
      121 taatccgcca ataaaagcaa tgtgtccatt ttgagtcaga atgactgaaa atgttaaccg
      181 ggcaataact gttttattgt aaaacaatga caacatcaac tctccttccc ggtcaaaacc
      241 actgggagaa cactggatat caaaattctc tccatctttc ccgatgaatt ggactaatga
      301 taccgatttt tgtggaaaca aaaatttaga atatttttct ggcaaatttt gcacaaagcg
      361 ataatgctca agaatatatt gtccacgggt ccaggcgcgc ccccctttat gcaaataagg
      421 gcgatgaatt cttgctggca acgtggggtg tattgccaga atatcctcaa tgcacggcaa
      481 ggaacgcagt tcataataat atcgaatact gacaaccgga tttatagaac atcgcaaaat
      541 gtacatgaac cgaaattttc tgtgttccca cttccccggt agtgaatctg aggaaaaaat
      601 aatcctttta aggaaaccga taaatgataa gttacttata gaaaacatat tg
//
LOCUS       DAJTRC010000315          643 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.329, whole genome shotgun sequence.
ACCESSION   DAJTRC010000315 DAJTRC010000000
VERSION     DAJTRC010000315.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 643)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 643)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..643
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.329"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..>643
                     /locus_tag="OO357_004290"
     CDS             <1..>643
                     /locus_tag="OO357_004290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_417523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888970.1"
                     /translation="RKVEAEREVRIKEIEQQQVTEIANQTKSIAIAAKSEQQSQAEAR
                     ANLALAEAVSAQQNVETTRQTAEADRAKQVALIAAAQDAETKAVELTVRAKAEKEAAE
                     MQAAAIVELAEATHKKGLAEAEAQRALNDAINVLSDEQTSLKFKLALLQALPAVIEKS
                     VEPMKSIDGIKIIQVDGLNSGSAAGDANTGNVGGGNLAEQALSAALSYRTQAPL"
BASE COUNT          197 a          159 c          174 g          113 t
ORIGIN      
        1 caagaaaggt tgaggctgaa cgtgaagttc gtattaaaga gatcgaacag cagcaggtca
       61 ccgaaatcgc caaccagacg aaatcgatcg ctattgccgc caaatcggaa caacagtccc
      121 aggcagaagc gcgtgctaac cttgcactcg cagaagcagt aagtgcgcaa caaaacgtag
      181 aaaccactcg ccagaccgca gaagccgatc gtgctaaaca agttgcccta atcgctgccg
      241 cgcaggatgc agaaaccaaa gcggttgaac tgaccgtgcg ggcgaaagca gaaaaagaag
      301 ccgcagagat gcaggcggcg gctatcgttg agttagccga agctacacat aaaaagggtc
      361 tggcggaagc agaagcacaa cgtgcgctga acgatgctat caacgtactt tctgatgaac
      421 aaaccagcct taaattcaaa ctggccttgt tgcaggcgct acctgcggta atagaaaaat
      481 ccgttgagcc gatgaaatct attgacggta tcaagattat tcaggtcgat ggtctgaata
      541 gtggcagcgc tgcgggtgat gcaaacacgg gtaatgtggg gggcggaaac ctagcggaac
      601 aagcattatc agccgctctc tcttaccgca cacaggctcc gct
//
LOCUS       DAJTRC010000316          639 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.330, whole genome shotgun sequence.
ACCESSION   DAJTRC010000316 DAJTRC010000000
VERSION     DAJTRC010000316.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 639)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 639)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..639
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.330"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            1..253
                     /locus_tag="OO357_004291"
                     /pseudo
     CDS             1..253
                     /locus_tag="OO357_004291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000899756.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          156 a          139 c          197 g          147 t
ORIGIN      
        1 tgccaacaca aaggcaacta ataaagatac agataaagat ctaaaaacag atctaaccca
       61 tcccaaaccc ttcccttccg gaagggagtt tcgggatttt gtggctggag tgcttgaggg
      121 gagattatct ggcggtactg cagcggaatt ttgtaattct gcggtggttg cgttgcaggc
      181 tgctggcctg gatgtctgtc gtgagtatcc ggtgccagag cgtggtgacg gttgcggagg
      241 gcggattgat atcgtcgtga ctgacaggaa cggtgtccgg tgtgggatcg agcttgaccg
      301 aaattctccg cgacagaaat cactgctcaa aatcggtgct gttgaaaccg ggatatgtgt
      361 cttgcggcgc agtgatatcg caaggcacac cgagcaggga attctggtta tcggtggggc
      421 tgttcgccag aaaaaatttg acccgttgtc agttgatctg cccgactggt tgtcagaaac
      481 actctggcat gagtgggtcc agttcaggca ggcattgcga aaaccgattc gaacggagct
      541 gggcgctaac ggggcgatac gggaactgga aaaattccgt cagcagggtt ttacacctga
      601 gcaggtgatt cgacacagca tcgccaatga ataccaggg
//
LOCUS       DAJTRC010000317          636 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.331, whole genome shotgun sequence.
ACCESSION   DAJTRC010000317 DAJTRC010000000
VERSION     DAJTRC010000317.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 636)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 636)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..636
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.331"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            19..492
                     /locus_tag="OO357_004292"
     CDS             19..492
                     /locus_tag="OO357_004292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HCR5888971.1"
                     /translation="MSWLISQCAHQCTDNKKTETDAIYDKVRSSYLLSCILKKNKNVG
                     LILHAPSFVSVSEKIARIVMANYSRNWSNSELASAVLMSESSLKRRMYKEVGSISTFV
                     HKIKLTEAIRKLRRTNTPISVISSELGYSSPSYFSKVFFKYLKTYPQNIRKKNGR"
BASE COUNT          210 a          113 c          128 g          185 t
ORIGIN      
        1 ggagatgaac cctatgcaat gtcctggtta atatctcagt gtgctcatca gtgcactgat
       61 aataaaaaga cggagacaga tgccatttac gataaagttc ggagttctta tttgctttca
      121 tgtattctga aaaaaaacaa aaatgttggg cttattcttc atgcaccatc atttgtttct
      181 gtgtctgaaa aaatcgccag aattgtgatg gcaaattact cccgtaactg gagtaattcc
      241 gagttggcca gtgcggtttt aatgagtgaa tcttctttaa aaagaagaat gtacaaagag
      301 gtcggttcta taagtacatt tgtacataaa ataaagctta cagaagctat caggaaatta
      361 cgaaggacaa atactcccat ttctgtcata tcatctgagt taggttacag ttcaccatcg
      421 tatttttcta aagtcttctt taaatattta aagacatacc ctcaaaacat aaggaagaaa
      481 aatggtcggt aatgtcacca attaggagga cttgcagagc cgctttttta cgatactgag
      541 atcaaacaga cataatctat gcggagattt acagcagggc atggttcaca aatctgacag
      601 cgatgaatta tcggcactgc gggcagaaaa tgctcg
//
LOCUS       DAJTRC010000318          630 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.332, whole genome shotgun sequence.
ACCESSION   DAJTRC010000318 DAJTRC010000000
VERSION     DAJTRC010000318.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 630)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 630)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..630
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.332"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..483)
                     /locus_tag="OO357_004293"
     CDS             complement(<1..483)
                     /locus_tag="OO357_004293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000935599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ash family protein"
                     /protein_id="HCR5888972.1"
                     /translation="MLNVAIENQNGWNYSASAPHKTGAGRGNPNVTRAHSRAEAVFLC
                     VMHSSIQIMVGCAGQSQDWPGSRVTGISTPVRLTTLMVVENLGGELINLSLEDAIMAT
                     IPALSHPDVTIENGRAVTTSVAVAEFFHKRHDNVLRAIANTECSPEFNALNFEDVTYT
                     D"
BASE COUNT          158 a          154 c          158 g          160 t
ORIGIN      
        1 gtcggtgtag gtgacgtcct caaaattgag ggcgttaaat tcaggtgagc attcagtatt
       61 tgcgatggca cgtaacacat tgtcgtgtcg tttgtggaag aactctgcaa ccgcaacaga
      121 cgtagtgaca gcgcgaccgt tttcgatggt tacgtcaggg tgagaaaggg cagggatagt
      181 agccatgatg gcatcctcga gtgataagtt aattaactca ccaccgaggt tttccacgac
      241 cataagggtg gtgagacgta caggggtgga aataccggtc actcgagaac ccggccagtc
      301 ttgcgactgc cctgcacatc ccaccataat ttgaatggag ctgtgcatta cgcataaaaa
      361 aaccgcttca gcgcggctat gcgctcgagt aacgttcggg tttccacgcc cggcccccgt
      421 tttatgaggt gcagatgcac tataattcca cccgttctgg ttttcaatag ctacattcaa
      481 cattttctct tacctttcat caccgaagtg aactttgttg atgcggtgcc tggtgcctcc
      541 aggtgacgtt aaccagttaa caattaacgc cggatacagg gaaacccata accacccgat
      601 acgtgggaac taatcgcttt ttaactgttc
//
LOCUS       DAJTRC010000319          628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.333, whole genome shotgun sequence.
ACCESSION   DAJTRC010000319 DAJTRC010000000
VERSION     DAJTRC010000319.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.333"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..>628)
                     /locus_tag="OO357_004294"
     CDS             complement(<1..>628)
                     /locus_tag="OO357_004294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001693020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HCR5888973.1"
                     /translation="TFGEREVFSDVTYNRLVEVLDEWIATRSNNNALELFAELRRFWK
                     FCAPTLCNGRNVAASLPDDYVSSRVQKPTPTRLFTDIESIARLWLNVAACTSVHQKNA
                     VRFMIITGVRPINVHNLRWDYVYEEAGEIVYPEGVIGMRGAMKTQKAFRLPITPEIRR
                     IIDEQKAWRDSVPECNRDYVFLQPRDPMQPFSKRSLDKLVKTYSPDGAV"
BASE COUNT          155 a          166 c          162 g          145 t
ORIGIN      
        1 acagccccgt ccgggctgta tgttttcacc agcttatcca gtgatcgttt tgaaaatggc
       61 tgcattggat cacgtggctg caaaaataca taatccctgt tgcactcagg aactgaatca
      121 cgccaggctt tctgctcgtc gataatccgc cggatctcag gcgttatcgg caggcggaaa
      181 gccttttgtg ttttcatagc ccctcgcatg ccgataaccc cttccggata aacaatttca
      241 ccagcctcct cgtaaacgta gtcccagcgc aggttatgga cattaatcgg acgaacacca
      301 gtgatgatca tgaagcgaac agcattcttc tggtgtacag aggtgcaggc agcaacattg
      361 agccagagtc gggcgattga ttcaatatcg gtaaaaagcc gtgtgggggt aggtttctgt
      421 acgcgggagg aaacataatc atctggcaga ctggcggcaa cattgcggcc gttgcaaaga
      481 gtaggtgcgc agaacttcca gaaccgacgg agctcggcaa acaactccag ggcgttattg
      541 ttcgagcgag tggcgatcca ctcgtccagc acttccacca gccgattgta tgttacgtcg
      601 ctgaacacct cacgctcgcc gaacgtta
//
LOCUS       DAJTRC010000320          621 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.334, whole genome shotgun sequence.
ACCESSION   DAJTRC010000320 DAJTRC010000000
VERSION     DAJTRC010000320.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.334"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..>621)
                     /locus_tag="OO357_004295"
     CDS             complement(<1..>621)
                     /locus_tag="OO357_004295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888974.1"
                     /translation="DGNPSTVTGIFPQGVRDVYRVTFEDGRYVDCAGDHLWEVTSRGF
                     TKGEKRRVIDTFGLKRLSETKRHKNGVRIPEITGDFGDHSEPLAWVIGSLLGDGSLSN
                     GSVKFSNVEPYMIERMKAELPDYNFSGDGKDWLISTARGQVNPLMETLRGYGLMGCTA
                     KNKFIPRVFFSANKSTRIGMLCGLLETDGYVEKDGTLVFSSASEELR"
BASE COUNT          156 a          179 c          125 g          161 t
ORIGIN      
        1 gcgcagttct tcacttgctg aggaaaaaac aagcgttcca tccttctcga catacccatc
       61 cgtttcaagc agaccacaca gcatgcctat acgcgttgat ttatttgcgg aaaaaaacac
      121 acgagggatg aatttgtttt ttgctgtgca ccccattagt ccataacctc gcagggtctc
      181 catgagtgga tttacctgac cacgcgccgt tgatatcagc cagtccttac catctccaga
      241 gaagttgtaa tcaggcagtt cagccttcat acgctcgatc atgtatggct cgacgtttga
      301 aaacttcacg ctcccattgc taagactacc atccccgaga agggagccga taacccatgc
      361 taatggctct gagtggtcgc caaagtcacc agttatttca ggaatcctaa cgccattttt
      421 gtgcctcttc gtttcactca accgcttcaa cccgaaggta tctatcacgc ggcgtttctc
      481 gcctttcgtg aatccacgac tagtgacctc ccataggtga tcgcctgcgc aatcaacata
      541 acgaccatct tcaaatgtga ctcggtaaac atctctaaca ccctgcggga atatcccagt
      601 gacagttgac ggattgccgt c
//
LOCUS       DAJTRC010000321          605 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.335, whole genome shotgun sequence.
ACCESSION   DAJTRC010000321 DAJTRC010000000
VERSION     DAJTRC010000321.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 605)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 605)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..605
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.335"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..132
                     /locus_tag="OO357_004296"
     CDS             <1..132
                     /locus_tag="OO357_004296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
                     /protein_id="HCR5888975.1"
                     /translation="LKVDPLKCELRGGQMRFTGLKRGYRLAEQVMMHEQLARIRWGG"
     gene            307..456
                     /locus_tag="OO357_004297"
                     /pseudo
     CDS             307..456
                     /locus_tag="OO357_004297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343765.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          150 a          127 c          172 g          156 t
ORIGIN      
        1 ctgaaggttg acccgctgaa gtgcgagctg cgcggaggtc agatgcggtt tacggggctg
       61 aagaggggtt accgtctggc agagcaggtt atgatgcatg agcagctggc tcgaatacgg
      121 tggggcggct gagagtcgca gaggggaagt tgcgtccatg ttgaggggaa cggagcaaaa
      181 aaccatcaga gatacattga atcaatcaat gtaagcaata ttaagtgtct ataccgatta
      241 cttgatgatc aggttgtgtt gaataaacgc gcattcttcg gtgctttttt aatcttttgg
      301 ggatttaaat tcctatcgat ggacatgaat gcaggctata taagagcagc gcgcatccac
      361 ttacccaatg cggttgagaa aatagccttc gatcgctttc atgtggcgaa acaaccgggt
      421 gaggtggtgg ataaaacccg ccagaatgaa ccgccccggt tttcctggag agtgttttat
      481 ctgtgaactc aggctgccag atcatcgttt ccgatggaag cataataagc tttttctgct
      541 tctgccggag ggatgtggcc cagcctttcc tgcaatcgtc gattgttata ccagtccacc
      601 cacgt
//
LOCUS       DAJTRC010000322          596 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.336, whole genome shotgun sequence.
ACCESSION   DAJTRC010000322 DAJTRC010000000
VERSION     DAJTRC010000322.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 596)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 596)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..596
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.336"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..216
                     /locus_tag="OO357_004298"
     CDS             <1..216
                     /locus_tag="OO357_004298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF535 family protein"
                     /protein_id="HCR5888976.1"
                     /translation="VYSDFWESVAGKTCGDWYRLPTQVIRKPLSDIASKKRSGYRKRY
                     ALLDYIHETTIRSLDAYPVHSEHQNLN"
     gene            281..>596
                     /locus_tag="OO357_004299"
     CDS             281..>596
                     /locus_tag="OO357_004299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5888977.1"
                     /translation="MKKYKSEFIPEFKKNYLSPVYWSTWFLLGMIAGISMFPPSFRDP
                     VLAKIGRWAGRLSKKARRRATINLSLCFPEKSDTEREIIVDKMFVTALQSIVMMAELA
                     IRG"
BASE COUNT          183 a          109 c          130 g          174 t
ORIGIN      
        1 gtctatagtg atttctggga gtctgtagca ggaaaaacct gtggtgactg gtacagatta
       61 ccaacccagg tgattcgaaa gccgctaagc gatatagcca gtaaaaaacg ctctgggtac
      121 cgaaaacgat acgctttgct ggattatatc catgaaacta ctatccgctc tttggatgca
      181 tatcctgtac actcagaaca tcagaattta aattaaggtt tgatgtattc tctgaataaa
      241 acagtaatga taattttggt agaggcattc gcactaaata atgaaaaaat ataaatctga
      301 gtttattcct gaatttaaga aaaattacct ttcccctgtt tactggtcta catggttcct
      361 tttgggaatg attgcaggta tttcaatgtt tcccccttca ttcagagatc ctgtcttggc
      421 aaaaataggg cgttgggcgg gcagattgag caaaaaagct cgtcgcaggg cgacgattaa
      481 tttatcgctt tgtttcccag aaaagagtga tacagaacgg gaaataattg tcgacaaaat
      541 gtttgtcaca gcattgcaat ccatagtgat gatggcagaa ctggcaattc gtggtc
//
LOCUS       DAJTRC010000323          595 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.337, whole genome shotgun sequence.
ACCESSION   DAJTRC010000323 DAJTRC010000000
VERSION     DAJTRC010000323.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 595)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 595)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..595
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.337"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..>595)
                     /locus_tag="OO357_004300"
     rRNA            complement(<1..>595)
                     /locus_tag="OO357_004300"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          126 a          179 c          141 g          149 t
ORIGIN      
        1 cggttacctt gttacgactt caccccagtc atgaatcaca aagtggtaag cgccctcccg
       61 aaggttaagc tacctacttc ttttgcaacc cactcccatg gtgtgacggg cggtgtgtac
      121 aaggcccggg aacgtattca ccgtggcatt ctgatccacg attactagcg attccgactt
      181 catggagtcg agttgcagac tccaatccgg actacgacgc actttatgag gtccgcttgc
      241 tctcgcgagg tcgcttctct ttgtatgcgc cattgtagca cgtgtgtagc cctggtcgta
      301 agggccatga tgacttgacg tcatccccac cttcctccag tttatcactg gcagtctcct
      361 ttgagttccc ggccggaccg ctggcaacaa aggataaggg ttgcgctcgt tgcgggactt
      421 aacccaacat ttcacaacac gagctgacga cagccatgca gcacctgtct cacggttccc
      481 gaaggcacat tctcatctct gaaaacttcc gtggatgtca agaccaggta aggttcttcg
      541 cgttgcatcg aattaaacca catgctccac cgcttgtgcg ggcccccgtc aattc
//
LOCUS       DAJTRC010000324          591 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.338, whole genome shotgun sequence.
ACCESSION   DAJTRC010000324 DAJTRC010000000
VERSION     DAJTRC010000324.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 591)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 591)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..591
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.338"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            32..493
                     /locus_tag="OO357_004301"
     CDS             32..493
                     /locus_tag="OO357_004301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708012.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YehR family lipoprotein"
                     /protein_id="HCR5888978.1"
                     /translation="MKAFNKLFSLVVASVLVFSLAGCGDKEESKKFSANLNGTEIAIT
                     YVYKGDKVLKQSSETKIQFASIGATTKEDAAKTLEPLSAKYKNIAGVEEKSTYTDTYA
                     QENVTIDMEKVDFKALQGISGINVSAEDAKKGITMAQMELVMKAAGFKEVK"
BASE COUNT          182 a          127 c          129 g          153 t
ORIGIN      
        1 agtttcttga ttaaataaaa tggagtttta catgaaggct ttcaataagc tgttttccct
       61 cgttgttgca tctgttctgg ttttctctct tgctggctgc ggtgacaaag aagaatcgaa
      121 gaaattcagc gccaatctga acggcactga aattgccatt acctatgtct acaaaggtga
      181 caaggtgctt aagcaatctt ctgaaaccaa aattcaattt gcctccattg gtgcaaccac
      241 caaagaagat gctgccaaga cacttgagcc gttaagcgcc aaatacaaaa acatcgcggg
      301 tgttgaagaa aaatcaacct ataccgatac ctacgcgcag gaaaacgtga ctatcgatat
      361 ggaaaaagtg gattttaaag ccctgcaggg tatttcagga atcaacgttt ctgctgaaga
      421 tgccaaaaaa ggtatcacta tggcgcaaat ggaactggtg atgaaagccg ctggttttaa
      481 agaagtgaaa taatctgtcg gcggccatgt ttcgcatggc cgccataccc gtcttagctt
      541 ttcttcacat gctggcgcgc tgccagtcca cgcagaaaat aacgtaaaaa t
//
LOCUS       DAJTRC010000325          585 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.339, whole genome shotgun sequence.
ACCESSION   DAJTRC010000325 DAJTRC010000000
VERSION     DAJTRC010000325.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 585)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 585)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..585
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.339"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..580)
                     /gene="agaC"
                     /locus_tag="OO357_004302"
     CDS             complement(<1..580)
                     /gene="agaC"
                     /locus_tag="OO357_004302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005188263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS N-acetylgalactosamine transporter subunit
                     IIC"
                     /protein_id="HCR5888979.1"
                     /translation="MTLLQGLSLAALVFVLGIDFWLEALFLFRPIIVCTLTGAILGDI
                     QTGLITGGLTELAFAGLTPAGGVQPPNPIMAGLMTTVIAWSTGVDAKTAIGLGLPFSL
                     LMQYVILFFYSAFSLFMTKADKCAKEADTAAFSRLNWTTMLIVASAYAVIAFLCTYLA
                     QGAMQALVKAMPAWLTHGFEVAGGILPAVGFGL"
BASE COUNT          178 a          154 c          143 g          110 t
ORIGIN      
        1 gcaagccaaa accaacggca ggcagaatac cgccagccac ttcaaagccg tgggtcaacc
       61 aggcgggcat cgctttcacc agcgcctgca tcgccccttg tgccaggtaa gtacagagga
      121 aagcaatcac cgcatacgct gaagcgacga tgagcatcgt tgtccagtta agccgggaaa
      181 acgctgccgt atccgcctct ttcgcgcatt tatcggcttt ggtcataaat aatgagaaag
      241 cggaatagaa gaacagaatg acgtactgca ttaacaaact aaacggcagg ccaagaccaa
      301 ttgctgtttt ggcatcaacg cccgtagacc atgcaatgac ggtggtcatc agacctgcca
      361 taatcgggtt gggcggctga acaccacctg caggggttaa tccggcgaaa gccaactctg
      421 tcagaccacc ggtaattaag ccagtctgaa tatcaccgag aatagcgcca gttagggtac
      481 aaacgattat cgggcggaat aaaaataagg cttccagcca aaaatcaatc cccagaacga
      541 aaactaacgc cgctagggat aatccctgaa gtagggttat ttcat
//
LOCUS       DAJTRC010000326          574 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.340, whole genome shotgun sequence.
ACCESSION   DAJTRC010000326 DAJTRC010000000
VERSION     DAJTRC010000326.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 574)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..574
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.340"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
BASE COUNT          166 a          148 c          130 g          130 t
ORIGIN      
        1 gacctttctt aaagtactcc aacctactga atcctaaggg atacggcttt aagcctcctt
       61 gcagagttac ttactgtaac ccactgtgta accctctcag ggcatgcaga catatgattc
      121 agctatccag tagtcacaaa cttccagcag tctactatct ctaccagcgt aacggcgtct
      181 actacttccg tcttagggtc agacaatcaa acaatgatag aatgacctca atatcattac
      241 ggaccaagga tagacgcacc gctatggctt actcaaggca cataaaggca gcactcaagg
      301 caatacacgc agacagaccc aacgctacct atgaggaaat gagggaacac cttaaggaca
      361 tcgctgagtg tgaactgtct atgggccgtt cagatctctt tgagcctgac atgagggata
      421 tctaccggga ccaatacgga gaacttggtg agagccttac cgatgctctg gctagtgagc
      481 cgctaagcat tgaccagcat cgttacatca atgaggccct gaaggtgcta aaagcctgta
      541 tgaggcgtat cgaagcggga gacagtcagc ctct
//
LOCUS       DAJTRC010000327          573 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.341, whole genome shotgun sequence.
ACCESSION   DAJTRC010000327 DAJTRC010000000
VERSION     DAJTRC010000327.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.341"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..566
                     /locus_tag="OO357_004303"
     CDS             <1..566
                     /locus_tag="OO357_004303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001173292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5888980.1"
                     /translation="GIKMKAETETGYEPSIRIQMEKQMNIETGQVWRTARILKDRSTR
                     IDGQVFSNPTFKNFLPHIESLNLGGEHPGIDTSRDNSELFANDGTPTWLKEKRAKEIA
                     LDEIIELLNKHHGGTSNDAKRAKADLLEKTFTSRSWERIKGMDWPTIKAGRNALWIEL
                     EGVEYAFPDPQTQNETQQAGYDENIPV"
BASE COUNT          202 a          135 c          127 g          109 t
ORIGIN      
        1 ccggtataaa aatgaaagca gaaacagaaa ccggttatga accctccatc cggatccaga
       61 tggagaagca aatgaacata gaaaccggtc aggtgtggcg caccgccaga atactaaaag
      121 atcgctccac ccgtattgat ggacaggtat tttcaaaccc aacatttaaa aactttcttc
      181 ctcatatcga gtctcttaac ctgggaggtg aacaccccgg catcgatacc tcacgggata
      241 acagtgaatt atttgccaac gacggtacac cgacatggct aaaagaaaag cgtgcaaaag
      301 aaatcgcact tgatgaaatt atcgagttgc tcaacaagca ccatgggggc accagtaacg
      361 acgccaaacg cgcaaaagct gatttgctgg aaaaaacatt cacctcccgc tcatgggaaa
      421 gaatcaaagg tatggactgg ccgacaatca aagctggtcg taatgcacta tggattgaac
      481 tggaaggcgt tgaatatgca ttcccggatc cacaaacaca aaacgaaacg caacaagcag
      541 gatacgatga gaatattccg gtgtaaaacc aag
//
LOCUS       DAJTRC010000328          570 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.342, whole genome shotgun sequence.
ACCESSION   DAJTRC010000328 DAJTRC010000000
VERSION     DAJTRC010000328.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 570)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 570)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..570
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.342"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
BASE COUNT          175 a          136 c          111 g          148 t
ORIGIN      
        1 taattgacaa taattttcat ttccattaaa aaagatcgcg tagcattctt aattcatgag
       61 gaactttacc cgccagcaat ctgagtagcg aaagctgttc atccccaatg tttttgctgg
      121 cgggtccttt tttctacggt cctcctcccc ggaggggggc atttcattat ttttctaatc
      181 gttactggtg agaaggcgga agaaaaagcc agttcttcgc ccctaaataa cagcatgatt
      241 ccagtccatg caaaatctga tttccagctc acacataccg agcactgtac aaaaaaccgc
      301 cagccaaggc agttaagagt gtattcccgg ggtttgctta gaatattttg taagttgtcc
      361 gaagagatat ttacaacacc agaatgatga atcatcagtc ccctgccaga ggttcactac
      421 gtaacctgac cgacaatgca ccattctggt gctgaaaaaa gagcactgga actgcaacaa
      481 aacaacatca cagaacagga gatggtaagg agtcagacat tcccacacaa tattgtgtca
      541 gtgcaaataa caaccttcgt ctcaaatctc
//
LOCUS       DAJTRC010000329          559 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.343, whole genome shotgun sequence.
ACCESSION   DAJTRC010000329 DAJTRC010000000
VERSION     DAJTRC010000329.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 559)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..559
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.343"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(338..>559)
                     /locus_tag="OO357_004304"
     CDS             complement(338..>559)
                     /locus_tag="OO357_004304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HCR5888981.1"
                     /translation="AGFAPIAIGLALTLIHLISIPVTNTSVNPARSTAVAIFQGGWAL
                     EQLWFFWVVPIVGGIIGGLIYRTLLEKRD"
BASE COUNT          135 a          169 c          148 g          107 t
ORIGIN      
        1 cctatctgag tgtttagcat tgttgcgctc ctttcatgtg caatgccata taaagccact
       61 ggcggaggga gcgcgcaggg ggcagccagt cgccgccgcc ccctgcaccc ccgggctctg
      121 gcgaacaaaa tcgccgctgc gcggtacctt cagcttatcc ccttcggcaa ccgggtcggg
      181 cgcgaggtaa cgtcctgtaa gacgcgccct cagcccacat ccatgtgggc tgccccggcc
      241 ttcggggaac gcttcagcga ttttgacgcc accaataccc gtgcggtttg taatgtcgaa
      301 gataaaacaa ccagggcaga taaaatgcca atgctaatta atcacgcttt tccagcagag
      361 tccggtaaat cagaccaccg ataatgccgc cgacaattgg caccacccag aagaaccaca
      421 gttgttctaa tgcccagccg ccctggaaga tagcaaccgc ggtgctgcgc gccgggttaa
      481 cagaagtgtt agtcaccgga atactgatta agtgaatcag ggttaaggcc agaccaatag
      541 cgatcggcgc aaaacctgc
//
LOCUS       DAJTRC010000330          558 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.344, whole genome shotgun sequence.
ACCESSION   DAJTRC010000330 DAJTRC010000000
VERSION     DAJTRC010000330.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 558)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 558)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..558
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.344"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(121..552)
                     /locus_tag="OO357_004305"
     CDS             complement(121..552)
                     /locus_tag="OO357_004305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016235410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HCR5888982.1"
                     /translation="MALNLASGEGNFFIRPGGVFYVAGDKVGIVRLDAFKASKDIQFA
                     VQSGPMLMENGVINPRIHPNVASRKIRNGVGINKHGNAVFLLSQQATNFYDFACYAKA
                     KLNVEQLLYLGGTISHMYMKGGAIPWQRYPFVTMISVERKG"
BASE COUNT          167 a          142 c          107 g          142 t
ORIGIN      
        1 ttatttggcc taaaaaatca ttcagattca ataaattgca acgtcatgta ggccggataa
       61 ggcgtttacg ccgcatccgg catcagcagc atatttagta tctgacacaa agctatcatt
      121 ctaacctttc cgctcgacgg aaatcatggt gacaaaagga tagcgttgcc acgggattgc
      181 cccgcctttc atatacatat gtgaaatcgt gccaccaaga taaagcagct gctcaacgtt
      241 cagtttcgct ttggcataac aggcaaaatc ataaaaattt gtcgcctgct ggctcaacaa
      301 aaacacggca ttcccatgtt tattaatccc aacaccgtta cgaattttac gtgaggcgac
      361 gttgggatga atacgcggat taattacacc gttttccatc aacattggtc ctgactgcac
      421 cgcaaactga atatctttac tggctttaaa agcatccaga cgaacgatgc cgactttatc
      481 tcccgcgaca taaaacacgc cgccaggacg gataaagaaa ttcccttcac ctgaagcgag
      541 atttaacgcc accttctg
//
LOCUS       DAJTRC010000331          548 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.345, whole genome shotgun sequence.
ACCESSION   DAJTRC010000331 DAJTRC010000000
VERSION     DAJTRC010000331.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 548)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..548
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.345"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
BASE COUNT          157 a          100 c          134 g          157 t
ORIGIN      
        1 ggtaaggcat cgcaactccg agcaaaaagc ggacttacag gatctgtgtt aaggctgtct
       61 tatgattcag acactatcgg ccgttatctg agaaatatac gagtaactgg taataacacc
      121 tgcaatggta ttgacacaaa cattacagca gaagactctg tcatcagaca ggtttatggc
      181 tgggtatttg ataatgtaat ggtgaatgaa gttgaaaccg cattatttaa tgcaagggct
      241 ctggcactca aaatttatag catgtcaggc tggaacctgt agagtcggtc ttcacttttt
      301 aggccagtgc gtaagtgtta gtgtcagctc ctgccatttc agcagaggaa attattctgc
      361 tgatgaaagc tttggcatca ggattcagcc tcaaacgtat gcgtggtcgt cagaggcagt
      421 taggtcagaa gcaataattt tagacagtga gaccatgtgc attggtttta aaaatgccgt
      481 ctatgttcat gattgccttg atttgcatat ggaacaactg gatttagatt attgcggctc
      541 aacaggcg
//
LOCUS       DAJTRC010000332          539 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.346, whole genome shotgun sequence.
ACCESSION   DAJTRC010000332 DAJTRC010000000
VERSION     DAJTRC010000332.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 539)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 539)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..539
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.346"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            1..539
                     /locus_tag="OO357_004306"
                     /pseudo
     CDS             1..539
                     /locus_tag="OO357_004306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoadenosine phosphosulfate reductase family
                     protein"
BASE COUNT          134 a          144 c          120 g          141 t
ORIGIN      
        1 aattctcttg caccattaac tatgttcagt ccctgcgcga gttgtacacc gatgtcatcg
       61 aagagtttta ctgggttgcg ctcccgctta cgacgcaaaa ttccctttca caataccaac
      121 ccgaatggca gtgctgggaa cctgatgtcg aatgggtgcg tcagcccccg caagatgcga
      181 taaccgctcc tgactttttc tgcttttacc agccaggcat gaccttcgaa caatttgtac
      241 gtgagtttgc cgaatggttt tcacaaaaac gtccggcggc gatgatgatc ggcatccgtg
      301 cggatgagtc ctacaaccgt tttgtcgcca tcgccagttt aaataaacaa cgttttgccg
      361 acgataaacc atggactacg gccgcaccag gcggtcatag ctggtacatt taccccattt
      421 acgactggaa agtggctgat atctggacct gatatgcaaa tcatcaaaat ctctgcaacc
      481 cactgtataa cctaatgtat caggcaggcg ttcctctgcg tcatatgcga atttgcgaa
//
LOCUS       DAJTRC010000333          533 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.347, whole genome shotgun sequence.
ACCESSION   DAJTRC010000333 DAJTRC010000000
VERSION     DAJTRC010000333.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 533)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 533)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..533
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.347"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..443
                     /locus_tag="OO357_004307"
     CDS             <1..443
                     /locus_tag="OO357_004307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3440 domain-containing protein"
                     /protein_id="HCR5888983.1"
                     /translation="HVIEPDRWAAMCARVSGVKSGGIYAGHDNHFYGHRKILKPEHLD
                     WQEYALLLLNSMPEKTAEHYRNKIAIYLHWYQKKGIEVPQTQQGDIGAKDIPSWRRIC
                     KVLLNNDYWCRALSFSPTKAKNYQRYNERIKGKRQEWGILCNND"
BASE COUNT          170 a          117 c          128 g          118 t
ORIGIN      
        1 gtcacgttat cgaaccggat cgctgggctg ctatgtgcgc acgagtcagc ggggtaaaaa
       61 gtggcggcat ttacgccgga catgacaatc atttctatgg gcatcggaaa atcctcaaac
      121 cagaacattt agactggcaa gaatatgcat tattgctgct caatagcatg ccggaaaaaa
      181 cagctgagca ttaccgcaat aaaatcgcca tttatttgca ctggtatcag aaaaaaggca
      241 tcgaggttcc acaaacccag caaggggaca ttggcgcgaa agatatcccc tcctggcggc
      301 ggatatgcaa agttttactc aataacgatt actggtgtcg ggcattatca tttagtccta
      361 cgaaagcgaa gaactatcag cgttataacg aacggataaa aggaaaacgt caggaatggg
      421 ggatactatg caacaacgat taacgcagga tttaacgcaa tttctcgcca gcttgccgga
      481 agatgatcgc atcaaagcga ttaatgaaat ccgcatggcg atccatcagg tta
//
LOCUS       DAJTRC010000334          532 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.348, whole genome shotgun sequence.
ACCESSION   DAJTRC010000334 DAJTRC010000000
VERSION     DAJTRC010000334.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.348"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..>532
                     /locus_tag="OO357_004308"
     CDS             <1..>532
                     /locus_tag="OO357_004308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="serine-type D-Ala-D-Ala carboxypeptidase"
                     /protein_id="HCR5888984.1"
                     /translation="IALADYVAGSQESFIGLMNGYAKKLGLTNTTFQTVHGLDAPGQF
                     STARDMALLGKALIHDVPEEYAIHKEKEFTFNKIRQPNRNRLLWSSNLNVDGMKTGTT
                     AGAGYNLVASATQGDMRLISVVLGAKTDRIRFNESEKLLTWGFRFFETVTPIKPDATF
                     VTQRVWFGDKIEVNLGA"
BASE COUNT          129 a          119 c          151 g          133 t
ORIGIN      
        1 gtattgcgct ggcagattac gttgccggga gccaggagtc ctttattggt ctgatgaatg
       61 gttatgccaa aaaactgggg ctgaccaaca ctaccttcca gacggtgcac ggcctggatg
      121 cgccggggca gttcagtacc gcgcgcgata tggcattgct gggtaaagca ttgatccacg
      181 atgtgccgga agagtacgcc attcataaag agaaagagtt caccttcaac aaaattcgtc
      241 agcctaaccg taaccgtctg ctgtggagca gcaatctgaa tgttgatggc atgaagacag
      301 gaaccacggc aggtgcggga tataatctgg ttgcttcggc tacccagggt gatatgcgtt
      361 taatctccgt agtgctgggg gcgaaaactg accgtatccg ttttaatgag tctgagaaat
      421 tattgacctg gggtttccgc ttctttgaaa ctgtgacgcc aattaaacct gatgccacct
      481 ttgtgactca gcgcgtctgg tttggtgata agatcgaagt gaatctcggg gc
//
LOCUS       DAJTRC010000335          524 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.349, whole genome shotgun sequence.
ACCESSION   DAJTRC010000335 DAJTRC010000000
VERSION     DAJTRC010000335.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.349"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(168..>524)
                     /locus_tag="OO357_004309"
     CDS             complement(168..>524)
                     /locus_tag="OO357_004309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707746.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antiterminator"
                     /protein_id="HCR5888985.1"
                     /translation="SICWEYQITITHWAWEEFKAHSGTRKIAEKTQERLKKLIWLAAQ
                     AVKAELFGGEGYEYQELALLAGVTTKNWSKTFTGHWVVMKHIFHRLDSEALLFVMRTR
                     SEQKAAFSKQSIAKVD"
BASE COUNT          135 a          135 c           89 g          165 t
ORIGIN      
        1 ataataacca tatgtcatca catctgcaaa ctcatctgac caccagcacg tttaacgtcc
       61 tgtgccattt ttcaggcata aaaaaaccgc tcggcggcgg gttttgctta ctttaccatc
      121 gcgtacaaaa tcggcaaagt atcagatttg catgagatat atgcctttta atctactttt
      181 gcaatacttt gctttgaaaa tgccgctttt tgttctgaac gtgttctcat cacaaacaat
      241 aaagcctcac tatccagtcg gtgaaaaatg tgtttcatta caacccagtg accagtaaat
      301 gttttggacc agtttttggt tgttactccc gccagtaatg ccagctcctg atattcataa
      361 ccttccccac caaaaagttc tgcttttacc gcctgcgccg ccagccagat taattttttc
      421 aggcgttcct gtgttttctc tgcaattttc ctagtgccag aatgagcctt aaactcttcc
      481 cacgcccagt gggttatggt gatctgatac tcccagcaaa tact
//
LOCUS       DAJTRC010000336          524 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.350, whole genome shotgun sequence.
ACCESSION   DAJTRC010000336 DAJTRC010000000
VERSION     DAJTRC010000336.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..524
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.350"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..499
                     /locus_tag="OO357_004310"
     CDS             <1..499
                     /locus_tag="OO357_004310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="non-ribosomal peptide synthetase"
                     /protein_id="HCR5888986.1"
                     /translation="DEVCEAHLATLLEQQPHGIAARLRARGEQVAFLGLLDTWPPETQ
                     NWQEKEANGLDPEVLAEINREREAFLAAQQGSTSTELFTTIEGNYADAVRLLTTAHSV
                     PFDGKATLFVAERTLQEGMSPERAWSPWIAELDIYRQDCAHVDIISPGAFVKIGPIIR
                     ATLNR"
BASE COUNT          125 a          126 c          156 g          117 t
ORIGIN      
        1 ggatgaagtc tgcgaagcgc atctggcaac gctacttgag caacaaccgc acggcattgc
       61 ggcgcggctg cgtgcccgtg gcgaacaggt ggcatttctt ggtttgctgg atacctggcc
      121 gccagagacg caaaactggc aggaaaaaga ggctaatggt ctggacccgg aagtgctggc
      181 ggagattaac cgcgaacgcg aggccttcct ggcggcacag cagggaagta cttcaacgga
      241 gttgtttacc accattgaag gcaactacgc tgatgctgtg cgcctgctga cgactgctca
      301 tagcgtaccg tttgatggta aagcgacgct gtttgttgct gaacgcacgc ttcaggaagg
      361 tatgagcccc gaacgcgcct ggtcgccgtg gattgcggag ctggatatct atcgtcagga
      421 ttgtgcgcat gtggatatta tctctccagg ggcgtttgta aaaattgggc cgattattcg
      481 cgcaacgcta aacaggtaaa ttaatattat ctataaaccc ataa
//
LOCUS       DAJTRC010000337          518 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.351, whole genome shotgun sequence.
ACCESSION   DAJTRC010000337 DAJTRC010000000
VERSION     DAJTRC010000337.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 518)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 518)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..518
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.351"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..416
                     /locus_tag="OO357_004311"
     CDS             <1..416
                     /locus_tag="OO357_004311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_462693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HCR5888987.1"
                     /translation="YSHRVFVALNEKPNVTAILCGGTYRARSDAFYDASNSSPLDSLN
                     PRKIFISASGVHNHFGVSWFNPEDLATKRKVMNRGLRKILLARHALFDEVASASLAPI
                     SAFDVLISDRPLPADYVTHCQNGSVKIITPDSEDE"
     gene            complement(418..>518)
                     /locus_tag="OO357_004312"
     CDS             complement(418..>518)
                     /locus_tag="OO357_004312"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312638.1"
                     /note="with IlvB catalyzes the formation of 2-acetolactate
                     from pyruvate, the small subunit is required for full
                     activity and valine sensitivity; E.coli produces 3
                     isoenzymes of acetolactate synthase which differ in
                     specificity to substrates, valine sensitivity and affinity
                     for cofactors; also known as acetolactate synthase small
                     subunit; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HCR5888988.1"
                     /translation="VISQIDKLEYVVKVQRNQSNPTMFNKIVVFFQ"
BASE COUNT          130 a          126 c          108 g          154 t
ORIGIN      
        1 gttactcaca tcgcgtcttt gttgcgttga atgaaaagcc taatgtaaca gcaatacttt
       61 gtggtggtac gtatcgtgcc agaagtgatg ctttttacga tgccagtaac tcttcgccat
      121 tagactctct caatccgcga aaaatattta tttccgccag cggtgtgcat aatcactttg
      181 gcgtcagctg gtttaaccct gaagatcttg ccactaagcg taaagtgatg aaccgtggac
      241 tacggaaaat tttgctcgcc cgccacgcgt tgttcgatga agtggcctct gccagcctcg
      301 caccgatctc tgcatttgac gttctgatta gcgatcgtcc gttaccggca gattatgtta
      361 cgcactgcca gaatggttct gtaaagatca ttacacctga ttcagaagac gaatgactta
      421 ctgaaaaaac accacaatct tgttaaacat cgtcgggttg gactgattac gttgcacttt
      481 caccacatat tccagcttat ctatttggct tatcacct
//
LOCUS       DAJTRC010000338          501 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.352, whole genome shotgun sequence.
ACCESSION   DAJTRC010000338 DAJTRC010000000
VERSION     DAJTRC010000338.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..501
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.352"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..>501)
                     /gene="btsS"
                     /locus_tag="OO357_004313"
     CDS             complement(<1..>501)
                     /gene="btsS"
                     /locus_tag="OO357_004313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="two-component regulatory system sensor histidine
                     kinase BtsS"
                     /protein_id="HCR5888989.1"
                     /translation="NEVNSMKVAQVLYQELDIGAVAITDREKLLAFTGIGDDHHLPGK
                     PISSTYTLKAIETGEVVYADGNEVPYRCSLHPQCKLGSTLVIPLRGENQRVMGTIKLY
                     EAKNRLFSSINRTLGEGIAQLLSAQILAGQYERQKAMLTQSEIKLLHAQVNPHFLFNA
                     LNTIKA"
BASE COUNT          121 a          127 c          117 g          136 t
ORIGIN      
        1 ccgctttaat ggtgttaagc gcattaaaca aaaaatgagg attcacctgg gcgtgaagca
       61 gtttgatttc tgactgggtg agcatcgctt tttgccgctc atattgccct gcaagaatct
      121 gcgccgaaag cagttgcgca atcccctcgc ccagcgtgcg gtttattgaa ctgaataacc
      181 ggtttttggc ttcatacaat ttgatggtgc ccatcacgcg ctgattttcg ccacgcaacg
      241 gaattaccag cgtcgacccc agtttgcatt gcggatgcaa agagcaacga taaggtactt
      301 cgttgccatc agcgtagacc acttcaccgg tttcaatcgc ttttaaggtg taagtcgaag
      361 aaatcggttt gccgggtaaa tggtggtcgt caccaattcc ggtaaaggcc agcaatttct
      421 ctcgatcggt aatcgcgact gcaccaatat ccagctcctg atacagtacc tgagccactt
      481 tcatgctgtt cacttcgtta a
//
LOCUS       DAJTRC010000339          488 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.353, whole genome shotgun sequence.
ACCESSION   DAJTRC010000339 DAJTRC010000000
VERSION     DAJTRC010000339.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 488)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 488)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..488
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.353"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            1..488
                     /locus_tag="OO357_004314"
                     /pseudo
     CDS             1..488
                     /locus_tag="OO357_004314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611435.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT          106 a          120 c          152 g          110 t
ORIGIN      
        1 ccgttgaata tggacgggca aaaaagtgga tagcgcacgc gcccttattg cccggggatg
       61 gggagtaagc ttagtcagcc gttgtctccg ggtgtcgcgt gcgcagttgc acgtcattct
      121 cagacgaacc gatgactgga tggatggccg ccgcagtcgt cacactgatg atacggatgt
      181 gcttctccgt atacaccatg ttatcggaga gctgcccacg tatggttatc gtcgggtatg
      241 ggcgctgctt cgcagacagg cagaacttga tggtatgcct gcgatcaatg ccaaacgtgt
      301 ttaccggctc atgcgccaga atgcgctgtt gcttgagcga aaacctgctg taccgccatc
      361 gaaacgggca catacaggca gagtggccgt gaaagaaagc aatcagcgat ggtgctctga
      421 cgggttcgag ttctgctgtg ataacggaga gagactgcgt gtcacgttcg cgctggactg
      481 ctgtgatc
//
LOCUS       DAJTRC010000340          484 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.354, whole genome shotgun sequence.
ACCESSION   DAJTRC010000340 DAJTRC010000000
VERSION     DAJTRC010000340.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 484)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 484)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..484
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.354"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            1..484
                     /locus_tag="OO357_004315"
                     /pseudo
     CDS             1..484
                     /locus_tag="OO357_004315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312839.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          177 a           81 c           96 g          130 t
ORIGIN      
        1 gtcgagtaat aatgaatatg acctgtttaa aaatagagaa acctcactgt ggaaacgcaa
       61 agtaccagaa agcgcagcgc tggcaatgac gggaacagta tataaattat ttggtaatcg
      121 cttactcaac gcccgcaatc agttagataa aaatctcctc gaaatggcta tgcaaatggt
      181 ttatggcaaa gatatggtga caaagtccgg ggatatattg attgatatac aattaaataa
      241 ggatggcagt ctccagagta agcactacac acttgatgta ggtgaagtta taaacagtta
      301 taatattgac cctctttgtg aatgcttcaa gtattaatca aacttactta catgataaaa
      361 atcctggtga cgttattagc ctgtatggca ctttagataa cattcccctt gtagttaagg
      421 aatgcacggg taaaaatcat caaattaatg ttaaagccaa attgccacaa gaacgaagtg
      481 aaaa
//
LOCUS       DAJTRC010000341          480 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.355, whole genome shotgun sequence.
ACCESSION   DAJTRC010000341 DAJTRC010000000
VERSION     DAJTRC010000341.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.355"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..>480)
                     /locus_tag="OO357_004316"
     CDS             complement(<1..>480)
                     /locus_tag="OO357_004316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HCR5888990.1"
                     /translation="VGACIVLMELMMMVQLWWSVWLCWPVLSLLIIGRGCGGLGAITT
                     GKDSQGKLPPAVYWLTLPWRIGMWLSMRWFCRRLEPVSKMTAGVYLRAFPRHIPAQNA
                     VLDVTFEFPRGRATKDRLYFCVPMLDLVVPEEGELRQAVAMLETLREEQGSVLVHCA"
BASE COUNT          129 a          153 c          103 g           95 t
ORIGIN      
        1 atgcgcaatg gaccagaacg ctgccttgct cttcgcgtaa tgtttccagc atcgccacgg
       61 cctgtcggag ctccccctct tccggaacca ccagatccaa catcggtaca cagaaataga
      121 gtcgatcttt tgtcgctcgt ccacgaggga attcaaaggt gacgtccaga accgcattct
      181 gtgccggaat atgtcgtgga aacgccctta aataaacacc agcagtcatt ttgctcaccg
      241 gctccaggcg acgacaaaac caacgcatag atagccacat cccgatgcgc cagggcaatg
      301 tcagccagta aacggcgggc gggagtttcc cctgactatc tttccctgtt gttatcgcgc
      361 caagcccacc gcacccacgg ccaataatga gtagcgataa tactggccaa cataaccaga
      421 ctgaccacca taactgaacc atcatcatta gctccatcaa cacaatgcac gcgcctacga
//
LOCUS       DAJTRC010000342          480 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.356, whole genome shotgun sequence.
ACCESSION   DAJTRC010000342 DAJTRC010000000
VERSION     DAJTRC010000342.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 480)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 480)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..480
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.356"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            1..480
                     /locus_tag="OO357_004317"
                     /pseudo
     CDS             1..480
                     /locus_tag="OO357_004317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019842110.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
BASE COUNT          136 a          125 c          112 g          107 t
ORIGIN      
        1 gagttcttcc ggaaaatgca caaaaacgtt attcagaaga tcgaaactct cgaatgctcg
       61 ccggagttta accggcttaa ttttaagccc gtcacctata ccgacgccaa aggcgaaaaa
      121 cgcccaatgt accaaatcac caaaaacggc ttcgttttcc tggtgatggg cttcactggg
      181 aaaaaagccg ctgcattcaa agaggcctac atcgctgatg ttcgaccgca tggaggcaga
      241 actgcaccag aataataccc cgtccgctga caagatgatt ccgggtaacg gtcgcactct
      301 ggttgtgcac ttcgacgaac acggcaatat cagattcacc gaaactgttc ctgacggtgc
      361 actggtctgt accctggata ctttccagtt ttatctggaa aaacaggggt gggctcttgt
      421 aaaccggagc gcaattaaaa atatgaccgt agagcagctg ctagactggc cccctgaatc
//
LOCUS       DAJTRC010000343          479 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.357, whole genome shotgun sequence.
ACCESSION   DAJTRC010000343 DAJTRC010000000
VERSION     DAJTRC010000343.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 479)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 479)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..479
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.357"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(87..479)
                     /gene="tcyP"
                     /locus_tag="OO357_004318"
                     /pseudo
     CDS             complement(87..479)
                     /gene="tcyP"
                     /locus_tag="OO357_004318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005120792.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cystine/sulfocysteine:cation symporter"
BASE COUNT          124 a          143 c          127 g           85 t
ORIGIN      
        1 atgagtcagt atgcatcgcc gcgatggaac atttaaaaag taaagcaacg gcccctatac
       61 aaaacggacc gttgccagca taagaattaa cggtgtgcca gttcggcgtc gtcttcgcta
      121 tccagaatgg ctttatcggt ttgcttcagc cactggctgg tcagcgtgcc agctgtcatc
      181 gagccactaa cgtttagcgc cgtacggccc atgtcgataa gcggttcaac ggagattaac
      241 agcgccacca gggttactgg caggcccatc gcaggcagca caatcagtgc ggcgaaagtt
      301 gcaccaccac cgacaccggc aacgcctgcg gaactaacgg taacaatacc gaccagcgtc
      361 gcaatccaca tcgggcccag cgggttaatg ccaaccgtag gcgcaaccat caccgcagca
      421 ttgccggata caaaccggcg cagccgttct gaccaatggt tgcaccgaaa gaggcggcg
//
LOCUS       DAJTRC010000344          475 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.358, whole genome shotgun sequence.
ACCESSION   DAJTRC010000344 DAJTRC010000000
VERSION     DAJTRC010000344.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..475
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.358"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..216
                     /locus_tag="OO357_004319"
     CDS             <1..216
                     /locus_tag="OO357_004319"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709057.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific DNA-methyltransferase"
                     /protein_id="HCR5888991.1"
                     /translation="GSFTTGAVAIASGRKFIGIEINSEYIKMGLRRLDVASHYSAEEL
                     AKVKKRKTGNLSKRSRLSEVDPDLIAK"
     gene            300..>475
                     /locus_tag="OO357_004320"
     CDS             300..>475
                     /locus_tag="OO357_004320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2556 family protein"
                     /protein_id="HCR5888992.1"
                     /translation="MIRKYWWLVVFAVFVFLFDTLLMQWIELLTTETDKCRNMNSVNP
                     LKLVNCDELNFQDR"
BASE COUNT          139 a           97 c          113 g          126 t
ORIGIN      
        1 ggtagcttta ctaccggtgc cgtagccatc gccagcggac gaaaattcat tggtattgag
       61 atcaacagcg agtacatcaa aatggggctt cgacggctgg atgtcgcgtc gcattactcc
      121 gcggaagaac tggcgaaagt gaaaaaaaga aagaccggca acctgtcaaa acgaagccgg
      181 ttaagcgaag ttgaccccga tctcattgca aagtaaagga agtgtaagcc tagttttcag
      241 attattcatt tcgtgtatat ttcctgccag acttggttaa acatgcacag gctcaggtaa
      301 tgattcgcaa gtattggtgg ctcgtcgttt tcgctgtctt cgtttttctg tttgatactt
      361 tactgatgca gtggattgaa ctgctgacaa cagaaacaga caaatgccgt aatatgaact
      421 cagttaatcc actaaaactg gttaactgtg acgaactgaa ttttcaggac agaat
//
LOCUS       DAJTRC010000345          472 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.359, whole genome shotgun sequence.
ACCESSION   DAJTRC010000345 DAJTRC010000000
VERSION     DAJTRC010000345.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 472)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 472)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..472
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.359"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..>472
                     /locus_tag="OO357_004321"
     CDS             <1..>472
                     /locus_tag="OO357_004321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000198547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase zinc-binding domain-containing
                     protein"
                     /protein_id="HCR5888993.1"
                     /translation="LDAGGLRDIEVEAVTKMLACGTRILGVKEYICDKPECPHVRYVT
                     NSCGSRACPSCGKKATDLWIATQLNRLPDCDWVHLVFTLPDTQWPVFESNRWLLNDVC
                     RLAVENLLYAARKRGQEPGIFCAIHTYGRRLNWHPHVHVSVTCGGLNKHGQWKKL"
BASE COUNT          101 a          130 c          144 g           97 t
ORIGIN      
        1 tcctggatgc gggcggtctg cgcgatatcg aggttgaggc tgtcacaaaa atgctggcct
       61 gcggcacgcg gatactgggc gtgaaggaat acatctgcga taaacctgag tgcccccacg
      121 tcagatacgt cactaactca tgcggcagcc gtgcctgccc gtcctgcgga aaaaaggcca
      181 cagacctgtg gatagcgaca cagctgaatc gtcttcctga ctgcgactgg gtacacctgg
      241 tcttcaccct gccggacacg cagtggccgg tgttcgaaag caaccggtgg ctgctgaatg
      301 acgtgtgccg tctggcggtg gagaatctgc tgtatgccgc ccggaaacgg gggcaggaac
      361 ccggtatctt ctgcgccatc cacacgtatg gccgtcgtct caactggcat ccgcatgtac
      421 atgtgtctgt aacctgtgga ggtctgaata agcatggtca gtggaaaaag ct
//
LOCUS       DAJTRC010000346          464 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.360, whole genome shotgun sequence.
ACCESSION   DAJTRC010000346 DAJTRC010000000
VERSION     DAJTRC010000346.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 464)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 464)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..464
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.360"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..446
                     /gene="cobU"
                     /locus_tag="OO357_004322"
     CDS             <1..446
                     /gene="cobU"
                     /locus_tag="OO357_004322"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_416497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HCR5888994.1"
                     /translation="ILDDEMAARIEHHRQSRPEHWRTVERWQHLDELIHADINPNEAV
                     LLECVTTMVTNLLFDYGGDKDPDEWDYQAMEQAINAEIQSLIAACQRCPAKVVLVTNE
                     VGMGIVPESRLARHFRDIAGRVNQQLAAAANEVWLVVSGIGVKIK"
BASE COUNT          121 a           86 c          140 g          117 t
ORIGIN      
        1 aaatccttga tgatgagatg gctgcacgga tagaacatca tcggcaaagc cgcccggagc
       61 actggcgcac agtggagcgc tggcaacatc ttgatgaatt aattcatgca gacattaacc
      121 cgaatgaggc tgtgttgctg gaatgcgtta ccacaatggt gactaatctg ttgtttgatt
      181 atggcggcga taaagaccct gatgaatggg attatcaggc gatggaacag gcgattaatg
      241 ctgagattca gtcgttgatt gctgcctgcc aacgttgccc cgcaaaggtt gtattagtga
      301 ctaacgaagt gggaatgggg attgtgccgg agagtcgtct ggcacgacat tttcgtgata
      361 ttgccgggcg ggtaaatcag cagttggccg ctgcggcaaa tgaagtatgg ctggtggttt
      421 cgggtattgg agtaaaaatc aaatgagcaa attattttgg gcga
//
LOCUS       DAJTRC010000347          456 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.361, whole genome shotgun sequence.
ACCESSION   DAJTRC010000347 DAJTRC010000000
VERSION     DAJTRC010000347.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 456)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 456)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..456
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.361"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(213..407)
                     /locus_tag="OO357_004323"
     CDS             complement(213..407)
                     /locus_tag="OO357_004323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004992548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulator"
                     /protein_id="HCR5888995.1"
                     /translation="MLARKGVETVLRLLDKETQELLQLEGRKRLAALMLVDMPDEQVR
                     EILEEIEASILSESVNAKAG"
BASE COUNT          133 a          104 c           88 g          131 t
ORIGIN      
        1 gggtaaaaaa agacgggatt tgatgggatt caataagtta tgcgccgatt gcattcaaga
       61 taaaacgagt taaaaatgct caatgcttcg atatcgataa aacattcaag ataggcgcgc
      121 cgtgtatttt aattctttta agactcttca aaaatgtcat cgtagccttt aaaaacgaat
      181 tttaaaggca ccctaaaggc aggtcaatga agttaacccg ccttcgcgtt tacactctct
      241 gagagaatac tagcttcaat ctcttccaaa atctctctga cttgctcgtc gggcatatca
      301 acgagcatca acgctgccag gcgtttacgg ccctctagtt gcagtagctc ttgagtttcc
      361 ttatctaaca agcgtagcac agtctcaaca cctttccttg cgagcatgtt tgtaagggct
      421 tctcgttcct cactggtaag aaacgacaac atttct
//
LOCUS       DAJTRC010000348          450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.362, whole genome shotgun sequence.
ACCESSION   DAJTRC010000348 DAJTRC010000000
VERSION     DAJTRC010000348.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.362"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            101..>450
                     /locus_tag="OO357_004324"
     CDS             101..>450
                     /locus_tag="OO357_004324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011787828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5888996.1"
                     /translation="MPIIAPISRDERRLMQKAIHKTHDKNYARRLTAMLMLHRGDRVS
                     DVARTLCCARSSVGRWINWFTQSGVEGLKSLPAGRARRWPFEHICTLLRELVKHSPGD
                     FGYQRSRWSTELLA"
BASE COUNT          111 a          125 c          116 g           98 t
ORIGIN      
        1 atagctgcgc ggaatagtag atccctttga gggaacttag cccggattgt gcgatctgat
       61 caatcgccaa atcaaaacaa atcaccaacc ggactgagca atgccgatca tagcaccaat
      121 ttcccgtgac gaacgacgcc tgatgcagaa agccatccat aaaacacacg ataaaaatta
      181 tgcccgcaga ctgactgcca tgctgatgct gcaccggggc gaccgtgtca gcgacgttgc
      241 cagaacgctc tgctgcgccc gttcctctgt tggacgctgg attaactggt tcacgcagtc
      301 gggtgttgag ggactgaaat cattacctgc cgggcgtgcc cgtcgctggc cgtttgagca
      361 tatctgcaca ctgttacgtg agctggtaaa acattctccc ggcgactttg gctaccagcg
      421 ttcacgctgg agtacagaac tgctggcaat
//
LOCUS       DAJTRC010000349          450 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.363, whole genome shotgun sequence.
ACCESSION   DAJTRC010000349 DAJTRC010000000
VERSION     DAJTRC010000349.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 450)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 450)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..450
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.363"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..281)
                     /locus_tag="OO357_004325"
     CDS             complement(<1..281)
                     /locus_tag="OO357_004325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HCR5888997.1"
                     /translation="MSDSQTLVVKLGTSVLTGGSRRLNRAHIVELVRQCAQLHAAGHR
                     IVIVTSGAIAAGREHLGYPELPATIASKQLLAAVGQSRLIQLWEQLFSI"
BASE COUNT          107 a          122 c          114 g          107 t
ORIGIN      
        1 taaatcgaaa acagctgttc ccacagttga atcagtcgac tctgccctac cgccgccagc
       61 agttgtttcg aggcgatagt cgctggcagt tccgggtaac ccaggtgctc acgtccggcg
      121 gcgatcgcgc ccgacgtcac aataacaatc cgatgcccgg cggcatgtaa ctgcgcgcac
      181 tggcgaacaa gttcaacgat atgggcacgg ttcagacggc gcgatccgcc tgttagcaca
      241 ctggtgccga gttttaccac cagcgtctgg ctgtcactca tgattctctg ccattcaatt
      301 ttaggaaaaa tgatatcaaa cgaacgtttt agcaggactg tcgtcggttg ccaaccatct
      361 gcgagcaaag catggcgttt tgttgcgcgg gatcagcaag cgtagcggca gttgtttacg
      421 cttttattac agatttaata aattaccaca
//
LOCUS       DAJTRC010000350          448 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.364, whole genome shotgun sequence.
ACCESSION   DAJTRC010000350 DAJTRC010000000
VERSION     DAJTRC010000350.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 448)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 448)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..448
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.364"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..193)
                     /locus_tag="OO357_004326"
     CDS             complement(<1..193)
                     /locus_tag="OO357_004326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 fimbrial protein"
                     /protein_id="HCR5888998.1"
                     /translation="MKRSIIAAAVFSSFFMSAGVFAADVDTGTLTIKGNIAESPCKFE
                     AGGDSVSINMPTVPTTVFEG"
BASE COUNT          170 a           70 c           62 g          146 t
ORIGIN      
        1 taccttcaaa gacagtggtt ggtacagtcg gcatattaat acttactgaa tcaccacccg
       61 cttcgaattt acacggagat tctgcaatat ttcctttaat ggttaatgtt ccggtatcaa
      121 cgtctgcagc aaatactcca gcgctcataa aaaaagaaga aaagacagcg gcagcaataa
      181 ttgaacgttt cataatatgt tccttaataa aatttatttt tggttgttaa gagtctgtat
      241 ctcttggaaa caaatttctc acatcaaaaa agctgccgca atgtaaacat attgttttat
      301 tgatttaaaa caaattaacc aatgcaatat aaatacaaca aatacttaag aatattaaat
      361 atttatcaca tctattaatt aatgtagatt tatgtaaatg gtattgttga tatcaataaa
      421 aaagacgaaa tcatttatat aaatgatt
//
LOCUS       DAJTRC010000351          434 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.365, whole genome shotgun sequence.
ACCESSION   DAJTRC010000351 DAJTRC010000000
VERSION     DAJTRC010000351.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 434)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 434)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..434
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.365"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            95..>434
                     /locus_tag="OO357_004327"
     CDS             95..>434
                     /locus_tag="OO357_004327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Specifically methylates the ribose of guanosine
                     2251 in 23S rRNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5888999.1"
                     /translation="MSEMIYGIHAVQALLERAPERFQEVFILKGREDKRLLPLIHALE
                     SQGVVIQLANRQYLDEKSDGAVHQGIIARVKPGRQYQENDLPDLIASLDQPFLLILDG
                     VTDPHNLGACL"
BASE COUNT          114 a          117 c          109 g           94 t
ORIGIN      
        1 gcctacataa tcacgcaaat tcaatatatt gcagagatca tgtaggccgg ataaacgtag
       61 tgcatcaggc aaaacgtaaa caacgagtac attaatgagc gaaatgattt acggcatcca
      121 cgcagtgcag gccctgctgg agcgcgcccc tgaacgtttt caggaagtct ttattttaaa
      181 aggccgtgaa gataaacgtc tgttaccgct gattcacgcc cttgagtctc agggcgtggt
      241 tatccagttg gcaaaccgcc aatatctcga cgagaaaagc gacggtgccg tgcatcaggg
      301 cattatcgcc cgcgtgaagc caggacgtca gtatcaggaa aacgatctgc cggatctgat
      361 cgcttcgctc gatcaaccgt tcctgctgat cctcgacggc gtaaccgatc cgcacaacct
      421 cggcgcgtgc ctgc
//
LOCUS       DAJTRC010000352          431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.366, whole genome shotgun sequence.
ACCESSION   DAJTRC010000352 DAJTRC010000000
VERSION     DAJTRC010000352.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.366"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
BASE COUNT          116 a           91 c           96 g          128 t
ORIGIN      
        1 cgccccattt ttaacgcctg actggctact gtctaaaata tttattttct gaatcacacc
       61 aaaaacaacg aaacaataat taaaaaagga ttctgttgtt gttaccggga accgacttat
      121 ttcgctcaga aagtcgttca accagctccg cagtgataag attcgctgat gatacagtgt
      181 atcctcactg cgtgctggag gtgccagtat aatatgttga tcaatacagt atttattgtg
      241 ttcagtcgtt tcctgatgcg tttggttctc tgcggattgt cgctggcgaa ataaatggct
      301 tgcggtaaat gccgttacag agcagcgcaa cgcgtcaatt ccttcggagt gcccgacttt
      361 tggcgagagt tccggtttta tgcgtccagg tagcggaaat ttcacccgaa tgagaaatgc
      421 attaataaaa t
//
LOCUS       DAJTRC010000353          431 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.367, whole genome shotgun sequence.
ACCESSION   DAJTRC010000353 DAJTRC010000000
VERSION     DAJTRC010000353.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..431
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.367"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            1..149
                     /locus_tag="OO357_004328"
                     /pseudo
     CDS             1..149
                     /locus_tag="OO357_004328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627495.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(169..>431)
                     /locus_tag="OO357_004329"
     CDS             complement(169..>431)
                     /locus_tag="OO357_004329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS4/IS5 family transposase"
                     /protein_id="HCR5889000.1"
                     /translation="STGRILVLSLLATLSTIVMWLLGYHAENKGLHLKYQANSIKSRR
                     VISYLTLAKNVLRHSPLILRRTVLSTVLNHLSRTYRNMVLVY"
BASE COUNT          110 a          104 c          101 g          116 t
ORIGIN      
        1 cgcgcgaagt gttgtatgac aatatgaaaa ctgtggttct gcaacgtgac gcatatcaga
       61 ccggtcagca ccggttccat ccttcgctgt ggcagttcgg caaggagatg ggcttctctc
      121 cccgactgtg tcgccccttc aggctgaggg atccccacaa aattactgct aataaaccag
      181 caccatattt cggtaggttc tggacagatg gtttagaact gtgctcagta ctgttcgtct
      241 taaaataagt ggcgagtgtc gaagaacatt cttcgctaac gtcagatatg agataacccg
      301 ccgggattta atgctgttag cctgatattt caggtgtaac cctttatttt cagcgtgata
      361 accgagtaac cacattacga tagtgctcaa cgttgccagc agactcagaa ccagaattct
      421 tcctgttgaa c
//
LOCUS       DAJTRC010000354          425 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.368, whole genome shotgun sequence.
ACCESSION   DAJTRC010000354 DAJTRC010000000
VERSION     DAJTRC010000354.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 425)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 425)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..425
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.368"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..233
                     /locus_tag="OO357_004330"
     CDS             <1..233
                     /locus_tag="OO357_004330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005160866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HCR5889001.1"
                     /translation="TKYLFFESQGIPDKVDIVSRLKTYGYSISGVETDDGYKALVRAF
                     QLHFRQKNYDGIMDAETAAILYALLEKYFPGK"
     gene            complement(315..425)
                     /locus_tag="OO357_004331"
                     /pseudo
     CDS             complement(315..425)
                     /locus_tag="OO357_004331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858162.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
BASE COUNT          101 a           90 c          112 g          122 t
ORIGIN      
        1 tgactaaata tttatttttt gaatcgcagg gcattccgga taaggttgat atcgtgtcac
       61 ggctgaagac ctatggatac agtatttctg gtgtggagac ggatgacgga tataaggcgc
      121 ttgtccgcgc ttttcagctt catttccggc agaaaaatta tgacggcata atggatgctg
      181 aaaccgctgc catcctgtat gcattgctgg agaagtattt cccgggtaaa taaggagtga
      241 gtcgtcagag ctgcgatata tctccttcct ccatgggctg tctcttatac acaaatcccc
      301 acccggggat ttgtgatgtc agatgtcccc agtaagacta tgttttcatt gcgctctatg
      361 aagctgagtg agcgtaacga ctggagctgc ttctgcggtg ctccggtggc gaacgtgaag
      421 tcata
//
LOCUS       DAJTRC010000355          415 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.369, whole genome shotgun sequence.
ACCESSION   DAJTRC010000355 DAJTRC010000000
VERSION     DAJTRC010000355.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 415)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 415)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..415
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.369"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..>415
                     /locus_tag="OO357_004332"
     CDS             <1..>415
                     /locus_tag="OO357_004332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HCR5889002.1"
                     /translation="IADELMTGCTNFVFSGKPGTGKNHLAAAIGNRLMAKGRSVIIVT
                     VSDVMSVLHESYDNGKSGEKFLQELCGVDLLVLDEIGMQRDTKNEQVVLNQIVDRRTA
                     SLRGVGMLTNINHAAMNTLLGERVMDRMVMNGGRWV"
BASE COUNT          101 a           88 c          133 g           93 t
ORIGIN      
        1 ccatagctga cgaactgatg acaggctgta cgaattttgt gttcagcggt aagcccggca
       61 ccggaaaaaa tcatcttgca gccgcgattg gcaaccggct gatggcgaag gggcgtagcg
      121 tgattatcgt caccgtgtcc gatgtcatga gcgtgttgca tgagagctac gacaacggca
      181 aatccggtga aaaattttta caggagcttt gtggtgttga cctgctggtc ctggatgaaa
      241 ttggcatgca gcgggatacg aaaaacgagc aggtggtact gaaccagatt gttgatcgcc
      301 ggacggcatc gttacgcggt gtggggatgc tgacaaatat taaccatgca gcgatgaata
      361 cacttctcgg cgagcgggtg atggatcgca tggtcatgaa cggcgggcgc tgggt
//
LOCUS       DAJTRC010000356          414 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.370, whole genome shotgun sequence.
ACCESSION   DAJTRC010000356 DAJTRC010000000
VERSION     DAJTRC010000356.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 414)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 414)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..414
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.370"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(141..410)
                     /locus_tag="OO357_004333"
     CDS             complement(141..410)
                     /locus_tag="OO357_004333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005101441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889003.1"
                     /translation="MLETDQAWDVFEKLEDCYFSQRDPSTPVSCQKSYDTRVLCYQRG
                     GVTVSTIQLRDDDIVISLESWLELARANGWFVVRRDKLVERLMQL"
BASE COUNT          114 a          107 c           79 g          114 t
ORIGIN      
        1 ctgtgccatt tttcaggcac aaaaaaaccc gcataaagcg ggttctttca agtgtccatg
       61 tctgctattc gcctcgcgat acagctttgc gaagcttacc gtaattgaag cagtttatgt
      121 gcaaaaatgc aagaactttt ttaaagctgc atcaaccttt ccaccagttt atctctgcga
      181 acaacaaacc aaccattggc tctcgccagt tccagccatg actcaaggga aataacaata
      241 tcatcatccc gtaactgaat tgtggaaaca gtgacaccgc ctctctgata acagagaact
      301 cgcgtgtcgt aacttttctg gcatgaaact ggcgttgacg gatccctttg actgaaatag
      361 cagtcttcca gtttttcgaa cacatcccac gcctgatcgg tttcgagcat tttg
//
LOCUS       DAJTRC010000357          413 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.371, whole genome shotgun sequence.
ACCESSION   DAJTRC010000357 DAJTRC010000000
VERSION     DAJTRC010000357.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 413)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 413)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..413
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.371"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..>413
                     /gene="pgrR"
                     /locus_tag="OO357_004334"
     CDS             <1..>413
                     /gene="pgrR"
                     /locus_tag="OO357_004334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_415844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-binding transcriptional regulator PgrR"
                     /protein_id="HCR5889004.1"
                     /translation="REEIADLMAFVVVAEERSFTRAAARLSMVQSALSQIVRRIEERL
                     GLRLLTRTTRSVVPTEAGEHLLSVLGPILHDIDSALTSLSDLQNRPSGTIRITTVEHA
                     AKTILLPAMRTFLKSHPEIDIQLTIDYGLTDVVSE"
BASE COUNT          100 a          104 c          103 g          106 t
ORIGIN      
        1 agcgtgaaga aatcgctgat ctgatggcgt ttgtcgtcgt tgcagaggag cgtagcttca
       61 ctcgcgcagc agcccgcctg agcatggtgc agtcagcttt aagccagata gtgcgccgta
      121 tagaagaacg attgggattg cggcttctga cgcgaaccac gcgcagcgtt gtacccactg
      181 aagcgggcga gcatcttttg tctgttcttg gccctatatt gcatgacata gattctgccc
      241 tgacatccct gagcgatctg cagaaccgcc catccgggac aatacgtatt actactgtag
      301 aacatgcagc aaaaacgata ttgttaccag ccatgcgcac attcctgaaa tcgcatcctg
      361 aaattgatat tcagcttacc attgattatg gtttgaccga tgtcgtttct gaa
//
LOCUS       DAJTRC010000358          410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.372, whole genome shotgun sequence.
ACCESSION   DAJTRC010000358 DAJTRC010000000
VERSION     DAJTRC010000358.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.372"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
BASE COUNT          103 a           86 c           88 g          133 t
ORIGIN      
        1 ggcctggtga atggagatgg gttaagcaaa gactctcgtt gctggtgtac tttttcgcgc
       61 tggcgctcaa gcatggataa tgcgattgcc tgtagttctt cgttagtcat tgtctgcacc
      121 tggaaagcaa tttaccggag tacctcacac tacggcaaaa atgttttatt gttgcaactt
      181 gcgtaattat tcaggtcagt ttggaacgca ctgtataaac ataaataacc tgatactgtt
      241 gtgtgttttc aaagaaaaca caggtatttt tcacataaac cttccccttt tcatattcga
      301 ccacttaatc aaaaaacggt tttatacttg ttgcccccac tggggttaaa tgtgagacat
      361 gtcgcacttt gtgccatgtg tcctcgctga ttttaggcgc ggttcattca
//
LOCUS       DAJTRC010000359          408 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.373, whole genome shotgun sequence.
ACCESSION   DAJTRC010000359 DAJTRC010000000
VERSION     DAJTRC010000359.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 408)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 408)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..408
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.373"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(52..408)
                     /locus_tag="OO357_004335"
                     /pseudo
     CDS             complement(52..408)
                     /locus_tag="OO357_004335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000105095.1"
                     /note="The RecE (also known as exonuclease VIII) pathway
                     is an alternative pathway for the initiation of homologous
                     recombination in Escherichia coli. The RecE pathway is
                     activated in recB, recC sbcA (suppressors of recB and
                     recC) mutants. Activation of the RecE pathway results in
                     the production of at least two phage proteins relevant for
                     recombinational repair. RecE is a duplex DNA-specific
                     exonuclease which produces 3' overhangs in duplex DNA by
                     preferentially degrading the 5'-ending strands.
                     Recombinational repair of E.coli chromosomal DNA involves
                     as well other proteins: RecA, RecF, RecJ, RecO, RecR, and
                     RuvC.; incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease VIII"
BASE COUNT           85 a          104 c          110 g          109 t
ORIGIN      
        1 gctgcttaaa aaaagaacaa atggtagtgt ccgctattgc cagtacacct cagccttaat
       61 tgcatcagtc aggcggagca gggcggcatt atccagagtt gtcggttctg ccatgccgcg
      121 tatggccaac agcagattct ggacatagct gttttcctga tccatctcaa gagcagtaat
      181 gtgtttgcgt tgttcacggg tggcatgatg caggtatttc agatccccgg cagcatacgt
      241 aaaaatgtgc agaagacgct gtgtgaaccg caaagtggct acagagactt cgcaatcctg
      301 gcaatcctcg tgggcgtctg cctgcgcgtt ttcttcctgg cctcccgcca gttctttggt
      361 ttcttgagca ttatcctggt ggtgaacgtc gtctggcgct gctcccgg
//
LOCUS       DAJTRC010000360          404 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.374, whole genome shotgun sequence.
ACCESSION   DAJTRC010000360 DAJTRC010000000
VERSION     DAJTRC010000360.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 404)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 404)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..404
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.374"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(1..404)
                     /locus_tag="OO357_004336"
                     /pseudo
     CDS             complement(1..404)
                     /locus_tag="OO357_004336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310468.2"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
BASE COUNT          119 a           97 c           72 g          116 t
ORIGIN      
        1 tcattaataa tctgcattgg tgttttgcca taatatcgtt gagtcgctcg cgtcaaatat
       61 tcctgtgatt tggctgacaa cgtcaccata ttctccagcg ccgattcact aaactgctct
      121 ttatcatgca tcttttctac cgtacttttc agccactacg gtacatcatc aatcacctgt
      181 tcttcgcggt aatgacgtaa acggttaatg acataaaaag taaccatctc aacaaactct
      241 tccagccctg tttcgcggaa atttaatgaa gaaatcactg tttccacata ggtgagaaaa
      301 gcgttattgg tccggtatac ctgcgaagca acaaagcaat aaggcaacaa cggcaggtaa
      361 tgctgctcaa aaaagcgttt actgatccca acgttcaata tgcg
//
LOCUS       DAJTRC010000361          399 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.375, whole genome shotgun sequence.
ACCESSION   DAJTRC010000361 DAJTRC010000000
VERSION     DAJTRC010000361.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 399)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 399)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..399
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.375"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(226..>399)
                     /locus_tag="OO357_004337"
     CDS             complement(226..>399)
                     /locus_tag="OO357_004337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889005.1"
                     /translation="SPLPVVLEWHCVMCEQFFHGKKFCEACGTGIYCRAVCTKSVYSY
                     QPDLFEDCGGAST"
BASE COUNT          108 a          115 c           92 g           84 t
ORIGIN      
        1 tcgcgtatgg ttgattgaca ccacagtgcg aattcggcga atgcgagatc acggtacgat
       61 tccgcaccac actaccaatc aataccccgg gttttattca ggtaagcagg actgcggata
      121 tccggtgtcg cgcctttgtg tcacgaacgg ggtgggtgcg aaacaccaga taaaatacag
      181 gttggcaata cctctgaacg ccctgagcag gtcgggcatt tcggattaag tactcgcacc
      241 tccgcagtcc tcaaacaagt ctggctggta gctgtaaaca gacttcgtac ataccgctct
      301 gcaataaatc cccgtaccac aggcctcaca gaacttcttc ccatgaaaaa actgctcaca
      361 catcacacaa tgccactcca gcacgaccgg taatggcga
//
LOCUS       DAJTRC010000362          392 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.376, whole genome shotgun sequence.
ACCESSION   DAJTRC010000362 DAJTRC010000000
VERSION     DAJTRC010000362.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 392)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 392)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..392
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.376"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..>392
                     /locus_tag="OO357_004338"
     CDS             <1..>392
                     /locus_tag="OO357_004338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001572122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbria/pilus periplasmic chaperone"
                     /protein_id="HCR5889006.1"
                     /translation="VFSKEEKIPYLIQAWVDPFNKEDKSKAPFTVIPPVSRLEPSQEK
                     ILRIIHTKGVSLPDDRESVFWLNIKNIPPSASNKATNSLEIAVKTRIKLFWRPANIRL
                     IPEDAAPKVKWRREGRNLIAENPNPIHI"
BASE COUNT          132 a           89 c           82 g           89 t
ORIGIN      
        1 tgtatttagc aaagaagaaa aaatacctta tctcatccag gcttgggtag atccatttaa
       61 taaagaggat aaaagcaaag cgccgtttac cgttattccg ccagtatcac gattagaacc
      121 ttcgcaagaa aaaatattac gcattattca caccaaaggt gtgtcattgc ctgatgatcg
      181 cgagtcagtc ttctggctga atattaaaaa tattccgcca tcagcgagca acaaagcgac
      241 gaactcgctg gaaatagcag taaaaacgcg tatcaagctg ttctggcgac cagcaaatat
      301 ccgccttatt cccgaagatg ctgcgccgaa agtgaaatgg cgccgggaag ggcgaaatct
      361 gatagcagag aatcccaacc caattcatat ca
//
LOCUS       DAJTRC010000363          391 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.377, whole genome shotgun sequence.
ACCESSION   DAJTRC010000363 DAJTRC010000000
VERSION     DAJTRC010000363.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 391)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 391)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..391
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.377"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            18..391
                     /locus_tag="OO357_004339"
                     /pseudo
     CDS             18..391
                     /locus_tag="OO357_004339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001371354.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
BASE COUNT          105 a           83 c          113 g           90 t
ORIGIN      
        1 agtttcctga ggttaaggtg ttaatcacgg cgacggattg caataaacgg tggttacagg
       61 aagttatcca ttttaatgtg ctggccattg tgcctcgcga ttcaaccgtc gagacatttg
      121 cgtaggcggt aaatagtgcg gcgatgggga tgatgtttct gcctggagac tggcggacga
      181 cgccggaaaa ggacattaaa gatctcaaat cgttaagcgc ccgccagcgg gagattttaa
      241 ccatgttagc ggcgggcgaa tcaaataagg agatcggcag agcgctgaat atcagtaccg
      301 gaacggtaaa agcgcatctg gaatccctgt atcgccgtct ggaagtgaaa aatcgcaccc
      361 aggcggcaat gatgttaaat atctcctcct g
//
LOCUS       DAJTRC010000364          389 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.378, whole genome shotgun sequence.
ACCESSION   DAJTRC010000364 DAJTRC010000000
VERSION     DAJTRC010000364.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 389)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 389)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..389
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.378"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..>389
                     /locus_tag="OO357_004340"
     CDS             <1..>389
                     /locus_tag="OO357_004340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HCR5889007.1"
                     /translation="FFGMVEGLNFALQYQGKNEGTGNYKANGDGHGLSATYTIDGFSF
                     AGAYANSDRTDWQSGDGKGERAEVWALSTKYDANNVYAAVMYGESHNMNSDDGDVVNK
                     TQNFEAVLQYQFDFGLRPSIGYSYSKAL"
BASE COUNT          108 a           77 c           90 g          114 t
ORIGIN      
        1 ttctttggca tggttgaagg tctaaatttt gctctgcaat atcagggtaa aaatgaaggt
       61 acaggcaact acaaagcaaa tggcgatggc catggtttat ctgcaactta cactattgac
      121 ggtttctcat ttgctggtgc ttatgcaaac tctgaccgta ctgattggca gtctggtgat
      181 ggtaaaggcg aaagagccga agtttgggcg ttgtcaacaa aatatgacgc taacaatgtt
      241 tatgctgctg tgatgtacgg tgaatctcac aacatgaatt ccgacgacgg tgatgttgtg
      301 aacaaaacac aaaacttcga agctgttctg caatatcagt tcgactttgg tctgcgtcca
      361 tccatcggtt actcctattc taaagcact
//
LOCUS       DAJTRC010000365          385 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.379, whole genome shotgun sequence.
ACCESSION   DAJTRC010000365 DAJTRC010000000
VERSION     DAJTRC010000365.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 385)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 385)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..385
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.379"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            1..385
                     /locus_tag="OO357_004341"
                     /pseudo
     CDS             1..385
                     /locus_tag="OO357_004341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001458895.1"
                     /note="internal stop; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
BASE COUNT          102 a           93 c          101 g           89 t
ORIGIN      
        1 ggtggtcaac aggagtgtga aatgaggcgc ttcgcaggcg catgtcgttt cgttttcaat
       61 cgtgctctgg cacgtcagaa tgagaatcat aaggccggta ataaatacat cccttacggg
      121 aaaatggctt cctggctggt tgagtggaaa aatgccactg aaacgcaatg gcttaaagat
      181 tctccctcac agccattgca acagtcactg aaagaccttg agcgggctta caaaaacttc
      241 ttccggaagc gggctgcttt tccccgattc aaaaagcggt gacagaatga tgcattccgc
      301 tacccgcagg gcgttaagct cgatcaggaa aacagccgta tttttctgcc gaaactgggc
      361 tggatgcgct accggaatag ccgtc
//
LOCUS       DAJTRC010000366          379 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.380, whole genome shotgun sequence.
ACCESSION   DAJTRC010000366 DAJTRC010000000
VERSION     DAJTRC010000366.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 379)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 379)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..379
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.380"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            52..327
                     /locus_tag="OO357_004342"
     CDS             52..327
                     /locus_tag="OO357_004342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707272.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889008.1"
                     /translation="MITSYEATVVTTDDIVHEVNLEGKRIGYVIKTENKETPFTVVDI
                     DGPSGNVKTLNDGVKKMCLVHIGKNLPAEKKAEFLATLIAMKLKGEI"
BASE COUNT          138 a           68 c           85 g           88 t
ORIGIN      
        1 tttatgccag caatggcagg gattcgctca accttaatta aggagaaaaa catgattacc
       61 agttatgaag ccactgttgt aactactgat gacattgttc acgaggttaa tctggaagga
      121 aagcgtattg gctacgtgat taagacagaa aataaagaaa ccccattcac tgtggttgat
      181 atcgacggtc catcaggcaa cgttaaaaca cttaacgatg gtgtaaaaaa aatgtgtctg
      241 gtgcacatag gaaagaatct gcccgcagaa aaaaaagccg aatttctggc aactctgatt
      301 gcaatgaaat taaaaggtga aatctgaaaa aaagaaagcc tgcacactgt gcaggcctga
      361 atgaagaacc tgggacatt
//
LOCUS       DAJTRC010000367          378 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.381, whole genome shotgun sequence.
ACCESSION   DAJTRC010000367 DAJTRC010000000
VERSION     DAJTRC010000367.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 378)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 378)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..378
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.381"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            14..378
                     /locus_tag="OO357_004343"
                     /pseudo
     CDS             14..378
                     /locus_tag="OO357_004343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612086.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DDE-type integrase/transposase/recombinase"
BASE COUNT          107 a           89 c          110 g           72 t
ORIGIN      
        1 catcgtgtgg tgacgagtct catcgtctat ggaaacgaca ttcgtgctgg atgcactgga
       61 gcaggcgttg tgggcccgtc gtccgtctgg caccatccat cacagcgata aaggctctca
      121 gtatgtgtca ctggcctata aggcgcgact aaaagaagca aaactgctgg catcaacagg
      181 gagtactggt gactcgtatg acaacgcgat ggcagagatc atcaagggcc tttacaaagc
      241 ggaggtaata caccgtaaga gctggaaaaa ccggacagaa gtggagctgg ccacactcac
      301 gtgggtggac tggtataaca atcgacgatt gctggaaagg ctgggccata tcccaccggc
      361 agaagcagaa aaagctta
//
LOCUS       DAJTRC010000368          374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.382, whole genome shotgun sequence.
ACCESSION   DAJTRC010000368 DAJTRC010000000
VERSION     DAJTRC010000368.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.382"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(41..100)
                     /locus_tag="OO357_004344"
     CDS             complement(41..100)
                     /locus_tag="OO357_004344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
                     /protein_id="HCR5889009.1"
                     /translation="MMKLVIILIVLLLVSFAAY"
BASE COUNT           84 a           97 c           90 g          103 t
ORIGIN      
        1 acaagggtga gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta
       61 cgagtaacaa cacaatcagt atgatgacga gcttcatcat aaccctttcc ttctgtaagg
      121 ccccttcctc gggaggggct ttcccgtttc agccctttgc tgactccccg attgttgatg
      181 agcgccggac ctcgcgcaac gctttcccag aggcagtttt gccttccccg agcggtcacg
      241 caaaacgttg caacgacctg atcatttgca gtttttagcg gaacgcagat ataaaaaatc
      301 cccggctttt tgtgccaggg acgttcatag ctgaaaattt attttacgtt ttcggcgacg
      361 ggagttgttg agat
//
LOCUS       DAJTRC010000369          374 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.383, whole genome shotgun sequence.
ACCESSION   DAJTRC010000369 DAJTRC010000000
VERSION     DAJTRC010000369.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..374
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.383"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..>374
                     /locus_tag="OO357_004345"
     CDS             <1..>374
                     /locus_tag="OO357_004345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HCR5889010.1"
                     /translation="DVTYIWTGKRWAYLAVVLDLFARKPVGWAMSFSPDSRLTMKALE
                     MAWETRGKPGGVMFHSDQGSHYTSRQFRQLLWRYQIRQSMSRRGNCWDNSPMERFFRS
                     LKNEWMPVVGYVSFSEAAHAIT"
BASE COUNT           89 a           91 c          121 g           73 t
ORIGIN      
        1 gatgtgacct atatctggac gggtaagcgc tgggcgtacc tcgccgttgt tctcgacctg
       61 ttcgcaagaa aaccagtggg ctgggccatg tcgttctcgc cggacagcag gctcaccatg
      121 aaagcgctgg aaatggcatg ggaaacccgt ggtaagcccg gcggggtgat gttccacagc
      181 gatcagggca gtcattatac gagcaggcag ttccggcagt tattgtggcg ataccagatc
      241 agacagagta tgagccggcg cggaaactgc tgggataaca gcccaatgga acgcttcttc
      301 aggagtctga agaacgaatg gatgccggtg gtgggttacg taagcttcag cgaggcagct
      361 cacgccataa cgga
//
LOCUS       DAJTRC010000370          371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.384, whole genome shotgun sequence.
ACCESSION   DAJTRC010000370 DAJTRC010000000
VERSION     DAJTRC010000370.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.384"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            89..>371
                     /locus_tag="OO357_004346"
     CDS             89..>371
                     /locus_tag="OO357_004346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HCR5889011.1"
                     /translation="MEITEPRRLYQQLAADLKERIEQGVYLVGDKLPAERFIADEKNV
                     SRTVVREAIIMLEVEGYVEVRKGSGIHVVSNQPRHQQAADNNMEFANYGP"
BASE COUNT          104 a           84 c           92 g           91 t
ORIGIN      
        1 caatcatcaa atttatatat gaccaatggc atacccatga ataaagtggt atgataactt
       61 cgtccgcatt ccactccggg agacgcatat ggaaatcact gaaccacgcc gtttgtatca
      121 acaacttgct gctgacctga aagagcgcat tgaacagggc gtctatctgg tgggtgataa
      181 actgcctgcc gaacgcttta ttgccgatga aaagaacgtc agccgaacgg tagttcgtga
      241 agccatcatc atgctggaag ttgaaggcta tgtggaggtg cgtaaaggtt caggtattca
      301 tgtggtttcc aaccagccgc gtcatcagca ggcggctgac aataatatgg aattcgccaa
      361 ttacggtccg t
//
LOCUS       DAJTRC010000371          371 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.385, whole genome shotgun sequence.
ACCESSION   DAJTRC010000371 DAJTRC010000000
VERSION     DAJTRC010000371.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..371
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.385"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..>371
                     /locus_tag="OO357_004347"
     CDS             <1..>371
                     /locus_tag="OO357_004347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001689732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HCR5889012.1"
                     /translation="SADTLQKVTQELNKQITAKGGWKAISESTQAQLGNLKNSWNTTL
                     DSIFRGSENGLQDFTRSLTNLLNALGGSGKSLGESLGSLMTSMSHGVDSLTDISYRVR
                     AFFDEVTLAYRPLNDTQRKIV"
BASE COUNT          113 a           69 c           84 g          105 t
ORIGIN      
        1 tctgccgata ctcttcagaa ggtcactcag gaactgaata aacaaatcac cgctaaaggt
       61 ggctggaaag ctatttcaga aagtacacag gcacaactag ggaacttgaa aaactcttgg
      121 aataccacgc ttgatagtat cttcagaggt tctgaaaatg gtctgcaaga ttttacaaga
      181 agcttaacca atcttttaaa cgctcttggt ggaagtggta agagtcttgg tgaatctctt
      241 ggtagtctca tgacttctat gtcacatggt gtcgattcat tgaccgatat tagttacagg
      301 gtaagggcgt tctttgatga ggtgacatta gcttaccgtc cactaaatga cactcagagg
      361 aaaattgttg a
//
LOCUS       DAJTRC010000372          370 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.386, whole genome shotgun sequence.
ACCESSION   DAJTRC010000372 DAJTRC010000000
VERSION     DAJTRC010000372.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 370)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 370)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..370
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.386"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            49..370
                     /locus_tag="OO357_004348"
                     /pseudo
     CDS             49..370
                     /locus_tag="OO357_004348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020238717.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
BASE COUNT           99 a           76 c           94 g          101 t
ORIGIN      
        1 ccatcttaaa tgactctgag gtgtctgtta aacccgtggc gattcagtga gcgcattctg
       61 accgaccgtg aaaatggtga aaaaggcgat aattctcaag atacctctta tgcccgtgta
      121 ggcgtgaaag gtgaaactca gatcaaccct gaaatgactg gttacggtca gtttgaacta
      181 gacctggaag catcaaacag acacaaccct gaccaaactc gtttggcata tgctggtttg
      241 agttacaaag attttggttc attcgactac agtcgtaatg tcggtgtcgc gtatgatgca
      301 gaagcattta ctgatatgtt tgttgagtgg ggcggtgact catgggctgg tactgacctg
      361 ttcatgacca
//
LOCUS       DAJTRC010000373          363 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.387, whole genome shotgun sequence.
ACCESSION   DAJTRC010000373 DAJTRC010000000
VERSION     DAJTRC010000373.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 363)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 363)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..363
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.387"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..348
                     /locus_tag="OO357_004349"
     CDS             <1..348
                     /locus_tag="OO357_004349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005069604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5889013.1"
                     /translation="IAPACTLVAATKDSVVTLPNVSATKLQTNGAVSGVKTDVPIALE
                     GCDVTVTKNATFTFSGTADGVQPTAFANQATTDAATNVALQMYLPDGSTSVTPGTETS
                     NIQLADSVMTPTY"
BASE COUNT           97 a           86 c           82 g           98 t
ORIGIN      
        1 attgctccag cttgtaccct ggtagctgcg acgaaagatt cagtagtcac attgccaaat
       61 gtcagcgcca ccaagttgca aactaatgga gcagtctctg gcgttaaaac tgatgtacca
      121 attgccttag aaggctgtga tgttactgta actaaaaatg cgacgtttac ctttagtggt
      181 acggctgacg gcgtgcaacc tacagcattt gccaaccagg ctaccaccga tgcagcgacg
      241 aatgttgctc tacaaatgta tctgccagat ggttctacat cggtaactcc cggtaccgaa
      301 acgtcgaata ttcagttagc ggattcggta atgactccaa cttactgata gtgttttatg
      361 ttc
//
LOCUS       DAJTRC010000374          360 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.388, whole genome shotgun sequence.
ACCESSION   DAJTRC010000374 DAJTRC010000000
VERSION     DAJTRC010000374.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 360)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 360)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..360
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.388"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(1..268)
                     /locus_tag="OO357_004350"
                     /pseudo
     CDS             complement(1..268)
                     /locus_tag="OO357_004350"
                     /inference="COORDINATES: protein motif:HMM:NF012786.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fimbria/pilus outer membrane usher protein"
BASE COUNT          107 a           93 c           63 g           97 t
ORIGIN      
        1 tataggggca aatccaatca ctaatggatt tactataacg aatacgatat ttattcccat
       61 aatataaatt cgcaatcgaa cgcgtaatat ctgtcgataa actccctaac agccccagag
      121 aaatgccaat acccgtagat attgcattaa aatgagtggc gttttgaatc cctccataca
      181 ccgtgacgcc ataaggaagc ccgtacatcg ccgtgatttc gcccacggag acaggggagc
      241 tttttatacc ttcttcttta tattcccccc catcacacta tatttgaaat atccttcatg
      301 cagagcgatg gccggcgttt gaaatggcac ggaaaaatac tgctcctcgc cattactctc
//
LOCUS       DAJTRC010000375          353 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.389, whole genome shotgun sequence.
ACCESSION   DAJTRC010000375 DAJTRC010000000
VERSION     DAJTRC010000375.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 353)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 353)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..353
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.389"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
BASE COUNT          132 a           58 c           57 g          106 t
ORIGIN      
        1 aacttatcgc atcctttcag gggcgggatg accaattaca gacaccaagc gttctactta
       61 aagtgaatgc ttcctgtgtg ctctgggtca gtcgataagt gaatagcaat aagacacaat
      121 catcttaatt atggtctcta gtccctacag gggcttaact taaagttaac ttaaagacac
      181 aattaaagat taacttatag tattacttaa agttaactta aagacacaat tagagattaa
      241 ctttaagtat taaataaaag atttaattaa agaccaatta aagattaact ctaagggaat
      301 accttaaggg gtgatgatat ttataataat caatcaccaa acctataggt ctt
//
LOCUS       DAJTRC010000376          351 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.390, whole genome shotgun sequence.
ACCESSION   DAJTRC010000376 DAJTRC010000000
VERSION     DAJTRC010000376.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 351)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 351)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..351
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.390"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
BASE COUNT           84 a           88 c           84 g           95 t
ORIGIN      
        1 cgttaccggc atttcgactg gttttatttc cgtacctgta tgtactggtg tgagcggaca
       61 aaaccactgt acgtctctca tacacgctcg taagcggccc tcacggcccg ctaacgcgga
      121 gatacgcccc gacttcgggt aaaaccctcg tcgggaccac tccgaccgcg cacaaaagca
      181 ttttcatcgc cgatagcggg tatggcttgg cagggccggg atgggtatag gtaatttggc
      241 tatcgtaaaa aaggtggtta aaaattacaa cctgcaatgt aaattggcta gttaattttt
      301 ggctatttaa attgacttct taacggcgtt tactgttcca ttgctcccca a
//
LOCUS       DAJTRC010000377          349 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.391, whole genome shotgun sequence.
ACCESSION   DAJTRC010000377 DAJTRC010000000
VERSION     DAJTRC010000377.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 349)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 349)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..349
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.391"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(155..>349)
                     /locus_tag="OO357_004351"
     CDS             complement(155..>349)
                     /locus_tag="OO357_004351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HCR5889014.1"
                     /translation="ITSRGGIHKDGPTDLVTPYLSTFLGFIGITDVKFVFAEGIAYGP
                     EMAAKAQSDAKAAIDSIVAA"
BASE COUNT           91 a           78 c           92 g           88 t
ORIGIN      
        1 aaacagttga tgaagcgatt atctttattc gagccagacg ctcgcatatt gtgcttaagg
       61 aagagcacaa agcgatgttg aaattatggg aaaacaggta agtggattga gatgtggact
      121 gaatatctac agtccacatc aagaccgtgt ccggttatgc agcaacaatg ctgtcgatgg
      181 ctgcttttgc gtcagactgt gctttcgctg ccatttccgg accgtatgcg atcccttcgg
      241 cgaagacaaa tttcacatcg gtaatgccga taaagccgag gaacgtggac agatacggcg
      301 tcaccaggtc cgttggtcca tctttgtgga tcccaccgcg gctggtaat
//
LOCUS       DAJTRC010000378          348 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.392, whole genome shotgun sequence.
ACCESSION   DAJTRC010000378 DAJTRC010000000
VERSION     DAJTRC010000378.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 348)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 348)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..348
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.392"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            163..>348
                     /locus_tag="OO357_004352"
     CDS             163..>348
                     /locus_tag="OO357_004352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_709061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HCR5889015.1"
                     /translation="MKKMMIATLAAASVLLAVANQAHAGATLDAVQKKGFVQCGISDG
                     LPRFSYADADGKFSGIDV"
BASE COUNT           88 a           76 c           88 g           96 t
ORIGIN      
        1 gggcgctgtt tttattattt cctgtctaca ctcagcttat tgcgacgcga aatcgtgcgc
       61 aaaagctggc actacttttg cttataagag gatggccaca gacaggtaaa agacgtttcc
      121 caaacgtcct ataacgataa ttttcgcctc acaggaagca ttatgaaaaa gatgatgata
      181 gccacactgg ctgccgccag cgtgctgctt gccgttgcaa atcaggcgca tgctggcgcg
      241 acgcttgatg ccgttcagaa aaaaggtttt gtgcaatgcg ggatcagtga tggattacct
      301 aggttctctt atgccgatgc agacggtaag ttttcaggta ttgatgtt
//
LOCUS       DAJTRC010000379          346 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.393, whole genome shotgun sequence.
ACCESSION   DAJTRC010000379 DAJTRC010000000
VERSION     DAJTRC010000379.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 346)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 346)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..346
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.393"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..230)
                     /locus_tag="OO357_004353"
     CDS             complement(<1..230)
                     /locus_tag="OO357_004353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310040.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889016.1"
                     /translation="MITERRNVLLQGAGWLLLLAPFFFFTYGSLNQFTAVQDLNSHDI
                     PSQVFGWETAIPFLPWTIVPYWSLDLLYGFSL"
     gene            complement(220..>346)
                     /locus_tag="OO357_004354"
     CDS             complement(220..>346)
                     /locus_tag="OO357_004354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase family
                     protein"
                     /protein_id="HCR5889017.1"
                     /translation="MRVRSQGEMDSLVRNAGFDKCTQRIDVWGIFTVSMAVRRDN"
BASE COUNT          113 a           93 c           76 g           64 t
ORIGIN      
        1 aacagcgaaa atccatataa aagatccaga ctccagtaag gaacaatagt ccagggaaga
       61 aaagggatcg ccgtttccca accgaatacc tgactgggga tatcatggct gttaaggtcc
      121 tgaaccgcgg tgaactgatt aagagatcca taggtgaaga agaaaaacgg ggccagcaac
      181 aataaccagc cagcgccttg tagcaatacg ttccgacgtt cagttatcac gacgcaccgc
      241 catcgaaacc gtaaaaatgc cccacacatc aatccgttga gtgcatttat caaatccggc
      301 attacgcacg agcgaatcca tctccccttg cgaacgcacg cgcatt
//
LOCUS       DAJTRC010000380          344 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.394, whole genome shotgun sequence.
ACCESSION   DAJTRC010000380 DAJTRC010000000
VERSION     DAJTRC010000380.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 344)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 344)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..344
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.394"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..>344
                     /gene="tuf"
                     /locus_tag="OO357_004355"
     CDS             <1..>344
                     /gene="tuf"
                     /locus_tag="OO357_004355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_312217.1"
                     /note="EF-Tu; promotes GTP-dependent binding of
                     aminoacyl-tRNA to the A-site of ribosomes during protein
                     biosynthesis; when the tRNA anticodon matches the mRNA
                     codon, GTP hydrolysis results; the inactive EF-Tu-GDP
                     leaves the ribosome and release of GDP is promoted by
                     elongation factor Ts; many prokaryotes have two copies of
                     the gene encoding EF-Tu; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HCR5889018.1"
                     /translation="AHVDCPGHADYVKNMITGAAQMDGAILVVAATDGPMPQTREHIL
                     LGRQVGVPYIIVFLNKCDMVDDEELLELVEMEVRELLSQYDFPGDDTPIVRGSALKAL
                     EGDAEWEAKILEL"
BASE COUNT           77 a           88 c          105 g           74 t
ORIGIN      
        1 gcacacgtag actgcccggg gcacgccgac tatgttaaaa acatgatcac cggtgctgct
       61 cagatggacg gcgcgatcct ggtagttgct gcgactgacg gcccgatgcc gcagactcgt
      121 gagcacatcc tgctgggtcg tcaggtaggc gttccgtaca tcatcgtgtt cctgaacaaa
      181 tgcgacatgg ttgatgacga agagctgctg gaactggttg aaatggaagt tcgtgaactt
      241 ctgtctcagt acgacttccc gggcgacgac actccgatcg ttcgtggttc tgctctgaaa
      301 gcgctggaag gcgacgcaga gtgggaagcg aaaatcctgg aact
//
LOCUS       DAJTRC010000381          343 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.395, whole genome shotgun sequence.
ACCESSION   DAJTRC010000381 DAJTRC010000000
VERSION     DAJTRC010000381.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 343)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 343)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..343
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.395"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..278
                     /locus_tag="OO357_004356"
     CDS             <1..278
                     /locus_tag="OO357_004356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_311977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HCR5889019.1"
                     /translation="IEDTTLELPLDSATTESLRAATHDVLAGLTAREAKVLRMRFGID
                     MNTDHTLEEVGKQFDVTRERIRQIEAKALRKLRHPSRSEVLRSFLDD"
BASE COUNT           76 a          104 c           96 g           67 t
ORIGIN      
        1 tcatcgagga taccaccctc gagctgccgc tggattctgc gaccaccgaa agcctgcgtg
       61 cggcaacgca cgacgtgctg gctggcctga ccgcgcgtga agcaaaagtt ctgcgtatgc
      121 gtttcggtat cgatatgaac accgaccaca cgctggaaga agtgggtaaa cagttcgacg
      181 ttacccgcga acgtatccgt cagatcgaag cgaaggcgct gcgcaaactg cgtcacccga
      241 gccgttctga agtgttgcgt agcttcctgg acgattaatc ggtaggccgg atcaggcgtt
      301 acgccgcacc cggcactagt ctctctgcac aaacgccacc ttt
//
LOCUS       DAJTRC010000382          341 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.396, whole genome shotgun sequence.
ACCESSION   DAJTRC010000382 DAJTRC010000000
VERSION     DAJTRC010000382.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 341)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 341)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..341
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.396"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..301
                     /locus_tag="OO357_004357"
     CDS             <1..301
                     /locus_tag="OO357_004357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313183.1"
                     /note="Specifically methylates the ribose of guanosine
                     2251 in 23S rRNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HCR5889020.1"
                     /translation="AAESVPLIRVTNLARTMRMLQEENIWIVGTAGEADHTLYQSKMT
                     GRLALVMGAEGEGMRRLTREHCDELISIPMAGSVSSLNVSVATGICLFEAVRQRS"
BASE COUNT           78 a           78 c          103 g           82 t
ORIGIN      
        1 cgcggcagaa agcgttccgc tgattcgggt gactaacctt gcgcgcacca tgcgtatgtt
       61 gcaggaagag aatatctgga tcgtcggtac ggcaggcgag gcggatcata cactctatca
      121 gagcaaaatg accggacgcc tggcgctggt gatgggcgcg gaaggtgaag gtatgcgtcg
      181 cctgacccgt gaacattgcg atgagttgat cagcatccca atggcaggaa gtgtttcttc
      241 cctgaacgtt tcggttgcaa ccggaatttg cttatttgaa gcggttcgtc agcgcagcta
      301 gttcctctga attatgaaaa gccatccaga tttggatggt t
//
LOCUS       DAJTRC010000383          340 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.397, whole genome shotgun sequence.
ACCESSION   DAJTRC010000383 DAJTRC010000000
VERSION     DAJTRC010000383.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 340)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 340)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..340
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.397"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..>340)
                     /locus_tag="OO357_004358"
     CDS             complement(<1..>340)
                     /locus_tag="OO357_004358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5889021.1"
                     /translation="KSRDNLGLKSAATMEAQSDIYDRTKGRLAIPGAFGFGCAFLPED
                     VIRFDTKSDFLAWVRNALPGEYSVAGPYGIIIPDTRFEGVLSIRWTDARPETTEPRYR
                     AKSLTFYGING"
BASE COUNT           76 a           97 c           88 g           79 t
ORIGIN      
        1 gccgttaatg ccgtaaaaag taagggattt ggctctgtac cgcggttctg ttgtctcagg
       61 gcgtgcatca gtccaccgga tgctgagcac cccttcaaac cgtgtgtcgg gtatgatgat
      121 gccgtagggg ccagcaacgg aatattcacc tggcagcgca ttccttaccc aggccaggaa
      181 atcactctta gtgtcaaaac ggataacatc ttcaggcaga aaagcacacc caaagccgaa
      241 tgcgccgggt atcgccagac ggccttttgt ccggtcgtaa atgtcgctct gtgcttccat
      301 cgtggccgca cttttcagcc ccagattatc ccgggacttc
//
LOCUS       DAJTRC010000384          339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.398, whole genome shotgun sequence.
ACCESSION   DAJTRC010000384 DAJTRC010000000
VERSION     DAJTRC010000384.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.398"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..>339)
                     /locus_tag="OO357_004359"
     CDS             complement(<1..>339)
                     /locus_tag="OO357_004359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_708404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HCR5889022.1"
                     /translation="FQNGVAIAFKNIQAGTARKFTLSSANNSTKNAAFYLWGNPSRPV
                     VAELGDDSGWHFFSQRNPDNSIVFTVNGQVIPLNYGNFDARYKYRTEGVQDVRYGHEM
                     YYSPGSNTVS"
BASE COUNT           89 a           83 c           72 g           95 t
ORIGIN      
        1 acgaaacggt gttactgccg gggctgtaat acatttcatg gccataccgt acatcctgta
       61 ccccctctgt tcgatattta tagcgggcat cgaagtttcc gtaatttaac ggaattacct
      121 gtccgttaac agtgaacact atgctgttat ccgggtttct ctggctgaaa aaatgccagc
      181 ctgaatcatc accaagctct gcaacaacag gcctggatgg attaccccac aaataaaacg
      241 ctgcattttt cgtggagttg ttggcgctgg ataacgtgaa ttttctggca gttccggcct
      301 gaatattctt aaaagcaata gccactccat tctgaaagc
//
LOCUS       DAJTRC010000385          339 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.399, whole genome shotgun sequence.
ACCESSION   DAJTRC010000385 DAJTRC010000000
VERSION     DAJTRC010000385.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 339)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 339)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..339
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.399"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..283
                     /locus_tag="OO357_004360"
     CDS             <1..283
                     /locus_tag="OO357_004360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HCR5889023.1"
                     /translation="YNWRVKETDRLFAMATELRKVGAEVEEGHDYIRITPPEKLNFAE
                     IATYNDHRMAMCFSLVALSDTPVTILDPKCTAKTFPDYFEQLARISQAA"
BASE COUNT           94 a           80 c           87 g           78 t
ORIGIN      
        1 ctataactgg cgtgttaaag agaccgatcg cctgtttgcg atggcaacag aactgcgtaa
       61 agtcggcgcg gaagtggaag aggggcacga ttacattcgt atcactcctc cggaaaaact
      121 gaactttgcc gagatcgcga catacaatga tcaccggatg gcgatgtgtt tctcgctggt
      181 ggcgttgtca gatacaccag tgacgattct tgatcccaaa tgcacggcca aaacatttcc
      241 ggattatttc gagcagctgg cgcggattag ccaggcagcc tgaatgaaca acgggcaata
      301 aatagccaaa tctttcttta tcaaaacgtc ggcacattg
//
LOCUS       DAJTRC010000386          335 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.400, whole genome shotgun sequence.
ACCESSION   DAJTRC010000386 DAJTRC010000000
VERSION     DAJTRC010000386.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 335)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 335)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..335
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.400"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..100
                     /locus_tag="OO357_004361"
     CDS             <1..100
                     /locus_tag="OO357_004361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1367 family protein"
                     /protein_id="HCR5889024.1"
                     /translation="SVLNVLWNWILFRKFSSPEEVENVAAQLLEFA"
     gene            100..>335
                     /locus_tag="OO357_004362"
     CDS             100..>335
                     /locus_tag="OO357_004362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_707251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1364 domain-containing protein"
                     /protein_id="HCR5889025.1"
                     /translation="MVDLRKAARGQMCTVRIPGYCNHNPETSVLAHYRLAGTCGTATK
                     PHDMQAAIACSSCHDLIDGRVKTSDYTKEELRLM"
BASE COUNT           87 a           79 c           95 g           74 t
ORIGIN      
        1 atctgtactg aatgtgctgt ggaactggat cctgttccgt aaattttcct ctccggagga
       61 agtcgaaaat gtggccgcgc agttactgga gtttgcgtaa tggtggattt acgtaaagcg
      121 gcgcgggggc agatgtgcac cgtcagaatt cctggctact gcaatcacaa tcccgaaact
      181 tctgtgctgg cgcattacag gctggcgggg acgtgcggaa cagcgacaaa accacacgat
      241 atgcaggcag cgattgcctg tagctcatgc cacgatttaa tcgacgggcg ggtaaaaacc
      301 agcgattaca ccaaagaaga attacgcctg atgca
//
LOCUS       DAJTRC010000387          332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.401, whole genome shotgun sequence.
ACCESSION   DAJTRC010000387 DAJTRC010000000
VERSION     DAJTRC010000387.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.401"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..>332
                     /locus_tag="OO357_004363"
     CDS             <1..>332
                     /locus_tag="OO357_004363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HCR5889026.1"
                     /translation="QEEDNERGVAHFVEHMMFNGTKTWPGNKVIETFESMGLRFGRDV
                     NAYTSYDETVYQVSLPTTQKQNLQQVMAIFSEWSNAATFEKLEVDAERGVITEEWRAH
                     QDAKWRTS"
BASE COUNT          102 a           67 c           88 g           75 t
ORIGIN      
        1 caggaagaag acaatgagcg cggcgtggct cattttgtag aacatatgat gtttaacggc
       61 acaaaaacat ggccgggtaa taaagtcatc gaaacatttg agtcaatggg cctgcgtttt
      121 ggtcgcgatg ttaatgccta taccagctat gacgaaacgg tgtatcaggt gagtttgccg
      181 actacgcaga aacaaaatct gcaacaagtg atggcaatct tcagtgaatg gagtaatgcc
      241 gcaacctttg aaaaactcga agtagacgct gaacgtggcg taattactga ggaatggcgt
      301 gcccatcagg atgcgaaatg gcgcacctct ca
//
LOCUS       DAJTRC010000388          332 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.402, whole genome shotgun sequence.
ACCESSION   DAJTRC010000388 DAJTRC010000000
VERSION     DAJTRC010000388.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 332)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 332)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..332
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.402"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
BASE COUNT          100 a           71 c           64 g           97 t
ORIGIN      
        1 ctgaccgggg tatccataaa acacatcatc gcccccagca aacatgcata catgaacaac
       61 aagccgcgat gggttttcgc gttcatgcag gcatagcgat tgatgtgcat atggtctcgc
      121 ctgactgaaa gtcttcagtc cgtacgtgcc acgttttgca acgagtatgt tgacacgtac
      181 atttccggaa tctggaaatc ggctactccc caaatgaggg cgcaacctta acatattttg
      241 agaatataaa acaactgttg gaatatatta tttcattgct ttattgataa cgattttcat
      301 ttatgtttat atatcaatga aacgttaaaa ta
//
LOCUS       DAJTRC010000389          331 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.403, whole genome shotgun sequence.
ACCESSION   DAJTRC010000389 DAJTRC010000000
VERSION     DAJTRC010000389.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 331)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 331)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..331
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.403"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(43..330)
                     /locus_tag="OO357_004364"
     CDS             complement(43..330)
                     /locus_tag="OO357_004364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889027.1"
                     /translation="MDEEYRKDLQLWFGLTHASFCVMPRVFMEAMPPEWQEKMAQLLF
                     EYSDTIKTDVCGVHSCFVTAKDGNNRFMRMPEDILNYRHPRREFIESFLKK"
BASE COUNT           91 a           85 c           66 g           89 t
ORIGIN      
        1 taagccatta tcaaacccac ccgtagatag gctttgtaat ggctacttct tcagaaatga
       61 ttcgatgaat tcacgtcgag gatgacgata gttcagaata tcttctggca tcctcataaa
      121 gcggttgttg ccgtctttgg cagtaacaaa acagctgtga actccgcaga catccgtttt
      181 gatcgtgtcg ctatactcaa aaagcaactg agccatcttc tcttgccatt ctggaggcat
      241 agcctccatg aatactcgcg gcatcacgca gaacgacgca tgcgtaagac caaaccacag
      301 ttgcaggtct ttacgatatt cttcatccat c
//
LOCUS       DAJTRC010000390          327 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.404, whole genome shotgun sequence.
ACCESSION   DAJTRC010000390 DAJTRC010000000
VERSION     DAJTRC010000390.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 327)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 327)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..327
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.404"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
BASE COUNT           91 a           74 c           82 g           80 t
ORIGIN      
        1 acgcatctgg ctggctaaag aagcgctgga atctggcgat tatgatagcg aagaagcctt
       61 ctacaaagca gaaggccgtg caggatattc accgggtctt ggcggggtat aaataccatg
      121 cgcattgact gaattcgcaa acaaaaacag acgcgcgata cccggatagt cggtctgcat
      181 agtcagatat ctggctgcga atttgataaa tatcaccgcc cttttcggaa acataagttc
      241 cgtccgggag ttgattatac gaaccatctc catgggggat cgttctcctg aatcctagtg
      301 agagcatata tttatgaagc ccttcgt
//
LOCUS       DAJTRC010000391          325 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.405, whole genome shotgun sequence.
ACCESSION   DAJTRC010000391 DAJTRC010000000
VERSION     DAJTRC010000391.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 325)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 325)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..325
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.405"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
BASE COUNT           70 a           92 c           79 g           84 t
ORIGIN      
        1 gattcaggca ccagaatcac ccgacgcccc ggaggcgctg ccggggtgtc gttccatttc
       61 tgatgatgac ctgtgcgcct ggtgtacccg tctgctttac cgtcccggag aaatcagtct
      121 gtgcagactg agtatacagg caatggcatc tggccatccg tctgtgaccg gaacggttac
      181 gcgtacggct gtccggaatt tcagccgaat atcattcatc cctgatatta ccgggtcatc
      241 acggtcagtg atgaacgtga tgttgccagc tcccgacctt tactccctct catttattca
      301 cattacaagg atttcataca tgaaa
//
LOCUS       DAJTRC010000392          323 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.406, whole genome shotgun sequence.
ACCESSION   DAJTRC010000392 DAJTRC010000000
VERSION     DAJTRC010000392.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 323)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 323)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..323
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.406"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(134..>323)
                     /locus_tag="OO357_004365"
     CDS             complement(134..>323)
                     /locus_tag="OO357_004365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1133 family protein"
                     /protein_id="HCR5889028.1"
                     /translation="RQRYEGRGMTKCKMAELLNDAHPKWSLRTCERRIEHWLKVAEFI
                     LYKPMVMAFGIEKKVIAF"
BASE COUNT           85 a           83 c           56 g           99 t
ORIGIN      
        1 acgtttaacg tcctgtgccg tttttcaggt ataaaaaaaa ccgcataaag cgggctcttt
       61 caaatgtcca tgtctgctat tcgcctcgcg gtacagcttt gcgaagcgta caagaattga
      121 agcagttttt acgtcaaaaa gcaataactt ttttctctat accaaaagcc ataaccattg
      181 gtttgtacaa aataaattct gccaccttta gccaatgctc aatgcgtctt tcacaggttc
      241 ttaaactcca tttcgggtgt gcatcattca gcagttcagc cattttgcat ttggtcatcc
      301 cccgcccctc ataccgttgc cga
//
LOCUS       DAJTRC010000393          322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.407, whole genome shotgun sequence.
ACCESSION   DAJTRC010000393 DAJTRC010000000
VERSION     DAJTRC010000393.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.407"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..>322
                     /locus_tag="OO357_004366"
     CDS             <1..>322
                     /locus_tag="OO357_004366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_858371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lauroyl-Kdo(2)-lipid IV(A) myristoyltransferase"
                     /protein_id="HCR5889029.1"
                     /translation="DQDHGPEYSEFADFFATYKATLPIIGRLMNISQAMIIPLFPVYD
                     EKKHLLTIEIRPPMDACIASADNKTIARQMNKTVEILVGPHPEQYVWVLKLLKTRKSN
                     EADP"
BASE COUNT          106 a           66 c           67 g           83 t
ORIGIN      
        1 cagatcagga tcatggtcct gaatacagtg aatttgctga tttttttgcg acctataaag
       61 cgacattacc aatcattgga cgtctgatga acatcagtca ggctatgatt ataccacttt
      121 tcccggttta tgatgaaaaa aaacatctac tgactattga aattcggcca ccaatggatg
      181 cttgcattgc cagcgcggac aataaaacga ttgcccgaca aatgaacaaa acagtggaaa
      241 ttttggtggg gccacatccg gaacagtatg tctgggtttt aaaattgtta aaaacacgca
      301 aatcaaacga agcggacccg ta
//
LOCUS       DAJTRC010000394          322 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.408, whole genome shotgun sequence.
ACCESSION   DAJTRC010000394 DAJTRC010000000
VERSION     DAJTRC010000394.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 322)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 322)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..322
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.408"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..170)
                     /locus_tag="OO357_004367"
     CDS             complement(<1..170)
                     /locus_tag="OO357_004367"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005127173.1"
                     /note="catalyzes the formation of pyridoxal 5'-phosphate
                     from pyridoxamine 5'-phosphate; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HCR5889030.1"
                     /translation="MSDNDELQQIAHLRREYTKGGLRRRDLPADPLTLFERWLSQACE
                     AKLADPTAMVVAT"
     gene            complement(229..>322)
                     /locus_tag="OO357_004368"
     CDS             complement(229..>322)
                     /locus_tag="OO357_004368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_310375.4"
                     /note="MliC; membrane-bound lysozyme inhibitor of c-type
                     lysozyme; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="lysozyme inhibitor"
                     /protein_id="HCR5889031.1"
                     /translation="YVFWSKGEEATVYKRDRIVLNNCQLQNPQR"
BASE COUNT           73 a           78 c           89 g           82 t
ORIGIN      
        1 gtagcgacca ccatcgcggt agggtccgcc agtttggctt cacaagcctg agagagccaa
       61 cgttcaaaaa gggttaatgg atcggcggga agatcgcggc ggcgtaaccc gcctttggtg
      121 tattcacggc gcagatgcgc gatttgctgc aattcgtcgt tatcagacat ggttttcttt
      181 acggattgtc agtgggtgac gctattgtgc gccgcccctg gaaaaatctc aacgctgtgg
      241 attttgtaac tgacagttat tcaagacgat gcggtcgcgt ttatagacag tcgcttcctc
      301 acctttcgac cagaaaacat ag
//
LOCUS       DAJTRC010000395          319 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.409, whole genome shotgun sequence.
ACCESSION   DAJTRC010000395 DAJTRC010000000
VERSION     DAJTRC010000395.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.409"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(31..89)
                     /locus_tag="OO357_004369"
                     /pseudo
     CDS             complement(31..89)
                     /locus_tag="OO357_004369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_002791256.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="type I toxin-antitoxin system Ibs family toxin"
BASE COUNT           70 a           95 c           72 g           82 t
ORIGIN      
        1 gggaggattt ctcccccctc tgattggctg ttaataagct gcgaaactta cgagtaacaa
       61 ccaaccagta tgatgacgaa cttcatcata accctttcct tctataaggc cccttcctcg
      121 ggaggggctt tcccgtttca gccccttgct gactccccga ttgttgatga gcgccggacc
      181 ccgcgcaacg ctttccctga agcagttttg ccttccccga gcggtcacgc aaaacgctgc
      241 aacgtcctga gcatttgcac cttttagcgg aacgcagata taaaaaatcc ccggcttttt
      301 gggccaggga tatgtgtta
//
LOCUS       DAJTRC010000396          319 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.410, whole genome shotgun sequence.
ACCESSION   DAJTRC010000396 DAJTRC010000000
VERSION     DAJTRC010000396.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..319
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.410"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..316
                     /locus_tag="OO357_004370"
     CDS             <1..316
                     /locus_tag="OO357_004370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_414677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="fimbrial protein"
                     /protein_id="HCR5889032.1"
                     /translation="GTENGQLLGNTLTGNDAAKGVGVLIEGLATSKNPLMTLKPNDSN
                     SVYKDYDPRGKDDTTGGVYPDQDTGITYPLHFQATLQQDGTIPIEAGEFKATSTFQVT
                     YP"
BASE COUNT          112 a           69 c           63 g           75 t
ORIGIN      
        1 tggtactgaa aacgggcaac tccttggtaa tactctcaca ggtaatgatg cagcaaaagg
       61 agtcggtgta ctcatagaag gtttagcaac tagtaaaaat cctctaatga cattgaaacc
      121 taatgattca aattctgttt ataaagatta cgacccgaga ggcaaagacg atacaacagg
      181 aggagtttac ccggatcaag atacaggtat aacataccct ctccatttcc aggccacgct
      241 acaacaggat ggaactatac caatagaagc tggtgagttt aaagccacca gtactttcca
      301 ggtaacctac ccttaataa
//
LOCUS       DAJTRC010000397          317 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.411, whole genome shotgun sequence.
ACCESSION   DAJTRC010000397 DAJTRC010000000
VERSION     DAJTRC010000397.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 317)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 317)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..317
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.411"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            1..317
                     /locus_tag="OO357_004371"
                     /pseudo
     CDS             1..317
                     /locus_tag="OO357_004371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001748481.1"
                     /note="frameshifted; incomplete; missing N-terminus and
                     C-terminus; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT           81 a           83 c           89 g           64 t
ORIGIN      
        1 cccgtgccgg tgtgccgggc agcgatgagg aaacccgtgc ggactttgaa aaccgccgac
       61 gtaattcagt tgcccgtaat gcccggaata ttctggaagc catccggggt gaaatactct
      121 ccacggtaga aaacgtggtg gatgtttacg tcacccataa tccgaaaaaa acagaacaaa
      181 aagccggagt cagtatccat taacacccgg ttcgctttat gttggcgtgt acggcggcag
      241 cccggcagat atcgcggcgg ccatctggcg taaggctccg ccgggtattg atatgaatgg
      301 caacacaacg ttcactg
//
LOCUS       DAJTRC010000398          315 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.412, whole genome shotgun sequence.
ACCESSION   DAJTRC010000398 DAJTRC010000000
VERSION     DAJTRC010000398.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..315
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.412"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            228..>315
                     /locus_tag="OO357_004372"
     CDS             228..>315
                     /locus_tag="OO357_004372"
                     /inference="COORDINATES: protein motif:HMM:NF025073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5889033.1"
                     /translation="MSAKNQDWHAEQIKAAVRMKGKTLSQLSR"
BASE COUNT           93 a           61 c           66 g           95 t
ORIGIN      
        1 ttatttccgg gggcataaaa atgcgctctg catttttgct ttctttttca cctgtcatgc
       61 ccaatctctc tgttatataa agaattttac agaattcttt gatttgcact ggttaaaata
      121 aaaatgcgca tagctttaga attgagcatt tttctgcgct atcgtttttc ccgatagcgg
      181 tagccggtat cagttaccga aaacgttaag agagtaagga tcgcagaatg tcagcaaaaa
      241 accaagactg gcacgcagag cagataaagg ctgctgttcg tatgaagggg aagacactat
      301 ctcaattatc cagag
//
LOCUS       DAJTRC010000399          309 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.413, whole genome shotgun sequence.
ACCESSION   DAJTRC010000399 DAJTRC010000000
VERSION     DAJTRC010000399.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 309)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 309)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..309
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.413"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
BASE COUNT           85 a           63 c           65 g           96 t
ORIGIN      
        1 cccccaggtt tataagtaca aacggttata tccacataga gacaaaaata ttaatctgat
       61 aaatatttat ttcacgccgt atatttgatt gtttaatgtg caagtacaat gacttttatt
      121 ttttgttgtg tatataatca aatatatggt tatttttcac cctgcgtatt cagcacgcaa
      181 caaaaaaccc gccgaagcgg gtttagtgcg ggtgcgttga ggatgcctga cacatgagag
      241 gtggcgaggg atttctcccc cgcctggtct cttactcctc aggttcgtaa gctgtgaaga
      301 cagcgacct
//
LOCUS       DAJTRC010000400          304 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.414, whole genome shotgun sequence.
ACCESSION   DAJTRC010000400 DAJTRC010000000
VERSION     DAJTRC010000400.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 304)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 304)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..304
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.414"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..>304
                     /locus_tag="OO357_004373"
     CDS             <1..>304
                     /locus_tag="OO357_004373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012904571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HCR5889034.1"
                     /translation="IEAEVRGCSAEQRLAARKARAAPLMQSLYDRIQQQMKTLSRHSD
                     TAKAFAYLLKQWKALNVYCSNGWVEIDNNIAENALRAVSLGRKNFLFFGSDHGGERG"
BASE COUNT           82 a           75 c           91 g           56 t
ORIGIN      
        1 ccatcgaggc agaagtccgg ggatgttcag cagaacagcg tctggcggca agaaaagcaa
       61 gagccgcgcc actgatgcag tcactgtatg accggataca gcaacagatg aaaacactgt
      121 cgcgtcactc ggatacggca aaagcgttcg catacctact gaaacagtgg aaggccctga
      181 acgtgtactg cagtaatggc tgggtggaaa tcgacaacaa catcgctgaa aatgccctgc
      241 gggcggtcag tctgggtcgt aaaaacttcc tgttcttcgg ctctgaccat ggtggtgagc
      301 gggg
//
LOCUS       DAJTRC010000401          302 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.denovo.415, whole genome shotgun sequence.
ACCESSION   DAJTRC010000401 DAJTRC010000000
VERSION     DAJTRC010000401.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.denovo.415"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..>302)
                     /locus_tag="OO357_004374"
     CDS             complement(<1..>302)
                     /locus_tag="OO357_004374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_309044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HCR5889035.1"
                     /translation="FPIYINTWHGIRNIDRGLVEMARSYGLSGIPLFIHVILPDALPS
                     IMVGVRFALGLMWLTLIVAETISANSGIGYLAMNAREFLQTDVVVVAIILYALLGK"
BASE COUNT           89 a           82 c           76 g           55 t
ORIGIN      
        1 gtttgccgag cagggcgtaa agaataatag cgactaccac cacgtctgtt tgcaaaaact
       61 ctcgcgcatt catcgccaga taaccaatgc cggaattggc agaaatggtt tcggcaacaa
      121 tcagcgtcag ccacatcagg cccaacgcaa aacgcacgcc gaccataatt gagggcaggg
      181 catcgggcag gatcacatgg ataaacagcg gtatgccgga taatccatag ctacgcgcca
      241 tctccaccag cccgcgatcg atattacgga tgccatgcca ggtgttgata taaatgggga
      301 ac
//
LOCUS       DAJTRC010000402         6307 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.guided.165, whole genome shotgun sequence.
ACCESSION   DAJTRC010000402 DAJTRC010000000
VERSION     DAJTRC010000402.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 6307)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6307)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6307
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.guided.165"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            89..1897
                     /gene="frdA"
                     /locus_tag="OO357_004375"
     CDS             89..1897
                     /gene="frdA"
                     /locus_tag="OO357_004375"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase (quinol) flavoprotein
                     subunit"
                     /protein_id="HCR5889036.1"
                     /translation="MQTFQADLAIVGAGGAGLRAAIAAAQANPNAKIALISKVYPMRS
                     HTVAAEGGSAAVAQDHDSFEYHFHDTVAGGDWLCEQDVVDYFVHHCPTEMTQLELWGC
                     PWSRRPDGSVNVRRFGGMKIERTWFAADKTGFHMLHTLFQTSLQFPQIQRFDEHFVLD
                     ILVDDGHVRGLVAINMMEGTLVQIRANAVVMATGGAGRVYRYNTNGGIVTGDGMGMAL
                     SHGVPLRDMEFVQYHPTGLPGSGILMTEGCRGEGGILVNKNGYRYLQDYGMGPETPLG
                     EPKNKYMELGPRDKVSQAFWHEWRKGNTISTPRGDVVYLDLRHLGEKKLHERLPFICE
                     LAKAYVGVDPVKEPIPVRPTAHYTMGGIETDQNCETRIKGLFAVGECSSVGLHGANRL
                     GSNSLAELVVFGRLAGEQATERAATAGNGNEAAIEAQAAGVEQRLKDLVNQDGGENWA
                     KIRDEMGLAMEEGCGIYRTPELMQKTIDKLAELQERFKRVRITDTSSVFNTDLLYTIE
                     LSHGLNVAECMAHSAMARKESRGAHQRLDEGCTERDDVNFLKHTLAFRDADGTTRLEY
                     SDVKITTLPPAKRVYGGEADAADKAEAANNKEKANG"
     gene            1890..2624
                     /gene="frdB"
                     /locus_tag="OO357_004376"
     CDS             1890..2624
                     /gene="frdB"
                     /locus_tag="OO357_004376"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020882957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase iron-sulfur protein"
                     /protein_id="HCR5889037.1"
                     /translation="MAEMKNLKIEVVRYNPEVDTAPHSAFYEVPYDATTSLLDALGYI
                     KDNLAPDLSYRWSCRMAICGSCGMMVNNVPKLACKTFLRDYTDGMKVEALANFPIERD
                     LVVDMTHFIESLEAIKPYIIGNSRTADQGTNIQTPAQMAKYHQFSGCINCGLCYAACP
                     QFGLNPEFIGPAAITLAHRYNEDSRDHGKKERMAQLNSQNGVWSCTFVGYCSEVCPKH
                     VDPAAAIQQGKVESSKDFLIATLKPR"
     gene            2635..3030
                     /gene="frdC"
                     /locus_tag="OO357_004377"
     CDS             2635..3030
                     /gene="frdC"
                     /locus_tag="OO357_004377"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdC"
                     /protein_id="HCR5889038.1"
                     /translation="MTTKRKPYVRPMTSTWWKKLPFYRFYMLREGTAVPAVWFSIELI
                     FALFALKNGPEAWAGFVDFLQNPVIVIINLITLTAALLHTKTWFELAPKAANIIVKDE
                     KMGPEPIIKSLWAVTVVATIVILFVALYW"
     gene            3041..3400
                     /gene="frdD"
                     /locus_tag="OO357_004378"
     CDS             3041..3400
                     /gene="frdD"
                     /locus_tag="OO357_004378"
                     /EC_number="1.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate reductase subunit FrdD"
                     /protein_id="HCR5889039.1"
                     /translation="MINPNPKRSDEPVFWGLFGAGGMWSAIIAPVMILLVGILLPLGL
                     FPGDALSYERVLAFAQSFIGRVFLFLMIVLPLWCGLHRMHHAMHDLKIHVPAGKWVFY
                     GLAAILTVVTLIGVVTI"
     gene            3463..4596
                     /locus_tag="OO357_004379"
     CDS             3463..4596
                     /locus_tag="OO357_004379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BlaEC family class C beta-lactamase"
                     /protein_id="HCR5889040.1"
                     /translation="MFKTTLCALLITASYSTFAAPQQINDIVHRTITPLIEQQKIPGM
                     AVAVIYQGKPYYFTWGYADIAKKQPVTQQTLFELGSVSKTFTGVLGGDAIARGEIKLS
                     DPATKYWPELTAKQWNGITLLHLATYTAGGLPLQVPDEVKSSSDLLRFYQNWQPAWAP
                     GTQRLYANSSIGLFGALAVKPSGLSFEQAMQTRVFQPLKLNHTWINVPPPEEKNYAWG
                     YREGKAVHVSPGALDAEAYGVKSTIEDMARWVQSNLPPLDINEKTLQQGIQLAQSRYW
                     QTGDMYQGLGWEMLDWPVNPDSIINGSDNKIALAARPVKAIKPPTPAVRASWVHKTGA
                     TGGFGSYVAFIPEKELGIVMLANKNYPNPARVAAACQILNALQ"
     gene            4685..5218
                     /gene="blc"
                     /locus_tag="OO357_004380"
     CDS             4685..5218
                     /gene="blc"
                     /locus_tag="OO357_004380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin Blc"
                     /protein_id="HCR5889041.1"
                     /translation="MRLLPLVAAATAAFLVVACSFPTPPRGVTVVNNFDAKRYLGTWY
                     EIARFDHRFERGLEKVTATYSLRDDGGLNVINKGYNPDRGMWQQSEGKAYFTGAPTRA
                     ALKVSFFGPFYGGYNVIALDREYRHALVCGPDRDYLWILSRTPTISDEVKQEMLAVAT
                     REGFDVSKFIWVQQPGS"
     gene            complement(5215..5532)
                     /gene="sugE"
                     /locus_tag="OO357_004381"
     CDS             complement(5215..5532)
                     /gene="sugE"
                     /locus_tag="OO357_004381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_418572.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary ammonium compound efflux SMR
                     transporter SugE"
                     /protein_id="HCR5889042.1"
                     /translation="MSWIILVIAGLLEVVWAVGLKYTHGFSRLTPSVITVTAMIVSMA
                     LLAWAMKSLPVGTAYAVWTGIGAVGAAITGIVLLGESANPMRLASLALIVLGIIGLKL
                     STH"
     gene            complement(5708..5854)
                     /gene="ecnB"
                     /locus_tag="OO357_004382"
     CDS             complement(5708..5854)
                     /gene="ecnB"
                     /locus_tag="OO357_004382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein toxin entericidin B"
                     /protein_id="HCR5889043.1"
                     /translation="MVKKTIAAIFSVLVLSTVLTACNTTRGVGEDISDGGNAISGAAT
                     KAQQ"
     gene            complement(5965..6090)
                     /gene="ecnA"
                     /locus_tag="OO357_004383"
     CDS             complement(5965..6090)
                     /gene="ecnA"
                     /locus_tag="OO357_004383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_710015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein antitoxin entericidin A"
                     /protein_id="HCR5889044.1"
                     /translation="MMKRLIVLVLLASTLLTGCNTACGFGEDIKHLGNSISRAAS"
     gene            complement(6142..>6307)
                     /locus_tag="OO357_004384"
     CDS             complement(6142..>6307)
                     /locus_tag="OO357_004384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_313155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HCR5889045.1"
                     /translation="IVDTDPGLKGDTAGTGGKPATLSTGAVVKVPLFVQIGEVIKVDT
                     RSGEYVSRVK"
BASE COUNT         1554 a         1645 c         1678 g         1430 t
ORIGIN      
        1 gcagtggaat agcgttcgca gaccgtaact ttcaggcact caccctgaag tacggggctg
       61 tgggataaaa acaatctgga ggaatgtcgt gcaaaccttt caagccgatc ttgccattgt
      121 aggcgccggt ggcgcgggat tacgtgctgc aattgctgcc gcgcaggcaa atccaaatgc
      181 aaaaatcgca ctaatctcaa aagtataccc gatgcgtagc cataccgttg ctgcagaagg
      241 gggctccgcc gctgtcgcgc aggatcatga cagcttcgaa tatcactttc acgatacagt
      301 agcgggtggc gactggttgt gtgagcagga tgtcgtggat tatttcgtcc accactgccc
      361 aaccgaaatg acccaactgg aactgtgggg atgcccatgg agccgtcgcc cggatggtag
      421 cgtcaacgta cgtcgcttcg gcggcatgaa aatcgagcgc acctggttcg ccgccgataa
      481 gaccggcttc catatgctgc acacgctgtt ccagacctct ctgcaattcc cgcagatcca
      541 gcgttttgac gaacatttcg tgctggatat tctggttgat gatggtcatg ttcgcggcct
      601 ggtagcaata aacatgatgg aaggcacgtt ggtgcagatc cgtgctaacg cggtcgttat
      661 ggctaccggc ggtgcgggtc gcgtttatcg ttacaacacc aacggcggca tcgttaccgg
      721 tgacggtatg ggtatggcgc taagccacgg cgttccgctg cgtgacatgg aattcgttca
      781 gtatcaccca accggtctgc caggttccgg tatcctgatg accgaaggct gccgtggtga
      841 aggcggtatt ctggtcaaca aaaatggcta ccgttatctg caagattacg gcatgggccc
      901 ggaaactccg ctgggcgagc cgaaaaacaa atatatggaa ctgggtccac gcgacaaagt
      961 ttctcaggcc ttctggcacg aatggcgtaa aggcaacacc atctccacgc cgcgtggtga
     1021 tgtggtttat ctcgacctgc gtcacctcgg cgagaaaaaa ctgcatgaac gtctgccgtt
     1081 catctgcgaa ctggcgaaag cgtacgttgg cgtcgatccg gttaaagaac cgattccggt
     1141 acgtccgact gcacactaca ccatgggcgg tatcgaaacc gatcagaact gtgaaacccg
     1201 cattaaaggt ctgttcgccg tgggtgaatg ttcctctgtt ggtctgcacg gtgcaaaccg
     1261 tctgggctcc aactccctgg cggaactggt ggtcttcggc cgtctggccg gtgaacaagc
     1321 gacagagcgt gcagcaactg ccggtaatgg caacgaagcg gcaattgaag cgcaggcagc
     1381 tggcgttgaa caacgtctga aagatctagt taaccaggat ggcggcgaaa actgggcgaa
     1441 gatccgcgac gaaatgggcc tggcaatgga agaaggttgc ggtatctacc gtacgccgga
     1501 actgatgcag aaaaccatcg acaagctggc agagctgcag gaacgcttca agcgcgtgcg
     1561 catcaccgac acctccagcg tgttcaacac cgacctgctc tacaccattg aactgagcca
     1621 cggtctgaac gttgctgaat gtatggcgca ctccgcaatg gcacgtaaag agtcccgcgg
     1681 cgcacaccag cgtctggacg aaggttgcac cgagcgtgac gacgtcaact ttctcaaaca
     1741 caccctcgcc ttccgcgatg ctgatggcac gactcgcctg gagtacagcg acgtgaagat
     1801 tactacgctg ccgccagcta aacgcgttta cggtggcgaa gcggatgcag ccgataaggc
     1861 ggaagcagcc aataataagg agaaggcgaa tggctgagat gaaaaacctg aaaattgagg
     1921 tggtgcgcta taacccggaa gtcgataccg caccgcatag cgcattctat gaagtgcctt
     1981 atgacgcaac tacctcatta ctggatgcgc tgggctacat caaagacaac ctggcaccgg
     2041 acctgagtta ccgctggtcc tgccgtatgg cgatttgtgg ttcctgcggc atgatggtta
     2101 acaacgtgcc aaaactggca tgtaaaacct tcctgcgtga ttacaccgac ggtatgaagg
     2161 ttgaagcgtt agctaacttc ccgattgaac gcgatctggt ggtcgatatg acccacttca
     2221 tcgaaagtct ggaagcgatc aaaccgtaca tcatcggcaa ctcccgcacc gcggatcagg
     2281 gtactaacat ccagaccccg gcgcagatgg caaagtatca ccagttctcc ggttgcatca
     2341 actgtggtct gtgctacgcc gcgtgcccgc agtttggcct gaacccagag ttcatcggtc
     2401 cggctgccat tacgctggcg catcgttata acgaagatag ccgcgaccac ggtaagaagg
     2461 agcgtatggc gcagttgaac agccagaacg gtgtatggag ctgtactttc gtgggctatt
     2521 gctccgaagt ctgcccgaaa cacgtcgatc cggctgcggc cattcagcag ggcaaagtag
     2581 aaagttcgaa agactttctt atcgcgaccc tgaaaccacg ctaaggagtg caacatgacg
     2641 actaaacgta aaccgtatgt acggccaatg acgtccacct ggtggaaaaa attgccgttt
     2701 tatcgctttt acatgctgcg cgaaggcacg gcggttccgg ctgtgtggtt cagcattgaa
     2761 ctgattttcg cgctgtttgc cctgaaaaat ggcccggaag cctgggcggg attcgtcgac
     2821 tttttacaaa acccggttat cgtgatcatt aacctgatca ctctgacggc agctctgctg
     2881 cacaccaaaa cctggtttga actggcaccg aaagcggcca atatcattgt aaaagacgaa
     2941 aaaatgggac cagagccaat tatcaaaagt ctctgggcgg taactgtggt tgccaccatc
     3001 gtaatcctgt ttgttgccct gtactggtaa ggagcctgag atgattaatc caaatccaaa
     3061 gcgttctgac gaaccggtat tctggggcct cttcggggcc ggtggtatgt ggagcgcaat
     3121 tattgcgccg gtgatgatcc tgctggtggg tattctgctg ccactggggc tgtttccggg
     3181 tgatgcgctg agctacgagc gcgttctggc gttcgcgcag agcttcattg gtcgcgtatt
     3241 cctgttcctg atgatcgttc tgccgctgtg gtgtggttta caccgtatgc accacgcgat
     3301 gcacgatctg aaaatccacg tgcctgcggg caaatgggtt ttctacggtc tggctgctat
     3361 cctgacagtt gtcacgctga ttggtgtcgt tacaatctaa cgcatcgcca atgtaaatcc
     3421 ggcccgccta tggcgggccg ttttgtatgg aaaccagacc ttatgttcaa aacgacgctc
     3481 tgcgccttat taattaccgc ctcttactcc acatttgctg cccctcaaca aatcaacgat
     3541 attgtgcatc gcacaattac cccgcttata gagcaacaaa agatccccgg tatggcggtg
     3601 gcggtaattt atcagggtaa accttattac tttacctggg gctatgcgga catcgccaaa
     3661 aagcagcccg tcacacagca aacgttgttt gagttaggtt cggtcagcaa aacatttacg
     3721 ggcgtgcttg gtggcgacgc tattgctcga ggggaaatca agttaagcga tcccgcaaca
     3781 aaatactggc ctgaacttac cgctaaacag tggaatggga tcacactatt acatcttgcg
     3841 acctacaccg ctggcggcct gccattgcag gtgccggatg aagtgaaatc ctcaagcgac
     3901 ttgctgcgct tctatcaaaa ctggcagcct gcatgggctc caggaacaca acgtctgtat
     3961 gccaactcca gtatcggttt gttcggcgca ctggctgtga agccgtctgg tttgagtttt
     4021 gagcaggcga tgcaaactcg tgtcttccag ccactcaaac tcaaccatac gtggattaat
     4081 gtacctcccc cagaagaaaa gaattacgcc tggggatatc gcgaaggtaa ggcagtgcat
     4141 gtttcgccag gggcgttaga tgctgaagct tatggtgtga agtcgaccat tgaagatatg
     4201 gcccgctggg tgcaaagcaa tttacccccc cttgatatca atgagaaaac gcttcaacaa
     4261 gggatacaac tggcacaatc tcgctactgg caaaccggcg atatgtatca gggcctgggc
     4321 tgggaaatgc tggactggcc ggtcaatcct gacagcatca ttaacggcag tgacaataaa
     4381 attgcactgg cagcacgccc ggtaaaagcg attaagcccc caactcctgc agtacgcgca
     4441 tcatgggtac ataaaacggg ggcgaccggc ggatttggta gctatgtcgc gtttattcca
     4501 gaaaaagagc tgggtatcgt gatgctggct aacaaaaact atcccaatcc agcgagagtc
     4561 gccgccgcct gtcagattct caacgctcta cagtaaaatt tcatcgggtc cgaattttcg
     4621 gaccttttct gcgcttttcc ttgctgtcat ctacacttag aaaaaaacca gtaaggaaac
     4681 atttatgcgc ctgctccctc tcgttgccgc agcgacagct gcatttctgg tcgttgcctg
     4741 tagttttcct acgccgccgc gtggcgtgac cgtagtgaat aatttcgacg ccaaacgtta
     4801 tctcggtacc tggtatgaga ttgcccgttt tgatcaccgt tttgaacgtg gactggaaaa
     4861 agtcaccgca acatacagcc tgcgtgatga cggcggcctg aatgtcatta ataaaggcta
     4921 taaccctgac agaggaatgt ggcagcagag tgaagggaaa gcgtacttta ccggcgcacc
     4981 aactcgcgct gcgctgaaag tgtcattctt tggtcctttc tatggcggtt ataacgttat
     5041 tgcactcgat cgggaatacc gccatgcgct ggtttgcggc ccggaccgcg actacctgtg
     5101 gatactctcc cgcacgccaa ccatttctga cgaagtgaaa caggagatgc tggcagtcgc
     5161 gacccgggaa gggtttgatg tcagtaagtt tatttgggta cagcagcccg gtagttagtg
     5221 agtgctgagt ttcagaccaa taatccccaa tacgattaac gccagactcg ccagacgcat
     5281 cgggttagcg gactcaccga gcagcacaat gccggtgatg gccgcgccga ctgcgccaat
     5341 acccgtccac acggcataag ccgtccctac cggtaacgat ttcatcgccc aggcaagtag
     5401 cgccatactg actatcatcg ccgtcacggt aataacactc ggcgtcaaac ggctaaagcc
     5461 gtgggtatat ttcaggccaa cggcccatac cacttccagc agaccagcaa taactaagat
     5521 aatccaggac atatcaggct ccggaaattt ggggccgtcc ccggtgaaaa gaggcgtttg
     5581 caggtcgtcc tgcaaagctg atggttgaac ggatattttg ccaggtggca ggggaatttc
     5641 aactattacc tttatgacga tcgcagaaaa aacaaacggc acgacactgc tgtcgtaccg
     5701 ttattgctta ttgctgcgct ttcgttgctg cgccagaaat cgcgttaccg ccatcagaaa
     5761 tgtcttcacc aacgccacgc gtggtgttgc aggcagttaa tactgttgaa agcaccagaa
     5821 cagaaaagat cgctgcaatt gtcttcttca ccataacgtc ttccttttag gtaatgttat
     5881 ttatgtttgc ctatagcaaa ttaataatag acaacatcgc caaaaatgat ggaaatctga
     5941 tgatttttcg gaagagaaga aaaattagct ggcagcgcga gagatggagt taccgagatg
     6001 tttgatgtct tcgccgaaac cgcaagcggt gttacagccc gtgagcagcg tgctggcaag
     6061 caaaacaaga acgataaggc gtttcatcat ctctgacctg aataagtgat ggtgcagcct
     6121 gcaggccgca ccacaaccgc attacttcac gcgagagacg tattcaccag agcgggtatc
     6181 cactttgatg acttcgccga tttgtacgaa cagcggaact ttaaccacag cgccagtaga
     6241 cagggtagcc ggtttgccgc cagtacctgc ggtatcacct ttcaggcccg gatcggtatc
     6301 aacgatt
//
LOCUS       DAJTRC010000403         5628 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.guided.172, whole genome shotgun sequence.
ACCESSION   DAJTRC010000403 DAJTRC010000000
VERSION     DAJTRC010000403.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 5628)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5628)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5628
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.guided.172"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            720..1535
                     /gene="sul2"
                     /locus_tag="OO357_004385"
     CDS             720..1535
                     /gene="sul2"
                     /locus_tag="OO357_004385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001043260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfonamide-resistant dihydropteroate synthase
                     Sul2"
                     /protein_id="HCR5889046.1"
                     /translation="MNKSLIIFGIVNITSDSFSDGGRYLAPDAAIAQARKLMAEGADV
                     IDLGPASSNPDAAPVSSDTEIARIAPVLDALKADGIPVSLDSYQPATQAYALSRGVAY
                     LNDIRGFPDAAFYPQLAKSSAKLVVMHSVQDGQADRREAPAGDIMDHIAAFFDARIAA
                     LTGAGIKRNRLVLDPGMGFFLGAAPETSLSVLARFDELRLRFDLPVLLSVSRKSFLRA
                     LTGRGPGDVGAATLAAELAAAAGGADFIRTHEPRPLRDGLAVLAALKETARIR"
     gene            1596..2399
                     /locus_tag="OO357_004386"
                     /note="aph(3'')-Ib"
     CDS             1596..2399
                     /locus_tag="OO357_004386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010890156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(3'')-Ib"
                     /protein_id="HCR5889047.1"
                     /translation="MNRTNIFFGESHSDWLPVRGGESGDFVFRRGDGHAFAKIAPASR
                     RGELAGERDRLIWLKGRGVACPEVINWQEEQEGACLVITAIPGVPAADLSGADLLKAW
                     PSMGQQLGAVHSLSVDQCPFERRLSRMFGRAVDVVSRNAVNPDFLPDEDKSTPQLDLL
                     ARVERELPVRLDQERTDMVVCHGDPCMPNFMVDPKTLQCTGLIDLGRLGTADRYADLA
                     LMIANAEENWAAPDEAERAFAVLFNVLGIEAPDRERLAFYLRLDPLTWG"
     gene            2399..3235
                     /locus_tag="OO357_004387"
     CDS             2399..3235
                     /locus_tag="OO357_004387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000480972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(6)-Id"
                     /protein_id="HCR5889048.1"
                     /translation="MFMPPVFPAHWHVSQPVLIADTFSSLVWKVSLPDGTPAIVKGLK
                     PIEDIADELRGADYLVWRNGRGAVRLLGRENNLMLLEYAGERMLSHIVAEHGDYQATE
                     IAAELMAKLYAASEEPLPSALLPIRDRFAALFQRARDDQNAGCQTDYVHAAIIADQMM
                     SNASELRGLHGDLHHENIMFSSRGWLVIDPVGLVGEVGFGAANMFYDPADRDDLCLDP
                     RRIAQMADAFSRALDVDPRRLLDQAYAYGCLSAAWNADGEEEQRDLAIAAAIKQVRQT
                     SY"
     gene            complement(3342..3746)
                     /locus_tag="OO357_004388"
                     /pseudo
     CDS             complement(3342..3746)
                     /locus_tag="OO357_004388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001120888.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS91 family transposase"
     gene            complement(3790..4179)
                     /locus_tag="OO357_004389"
     CDS             complement(3790..4179)
                     /locus_tag="OO357_004389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000102614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HCR5889049.1"
                     /translation="MSTELNSGTVGGFGHMGPNDPYNDGHSGSGSSGGSFNGGGNGGH
                     DNSSGSSYTASFGLITGRHPITVPGTKNINSVDLSAVINNIIHHEGARNNMYLDTANN
                     VRVGVTGTGWQCLATAGISAEGKRTFR"
     gene            4397..4837
                     /locus_tag="OO357_004390"
     CDS             4397..4837
                     /locus_tag="OO357_004390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001749639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="entry exclusion protein 1"
                     /protein_id="HCR5889050.1"
                     /translation="MAWHTVQETLKLTGKSRSQLYRDMAKGLVSYRTGKNDRREFETS
                     ELIRAYGELRTSETPKRHKEGHSKSVVSQAENAQLDVIQQKLDALQQTVTLMLEDKMA
                     REAENRLRNEEREQLQAEISRLADALAQEKKRGFWSRLFRSGSE"
BASE COUNT         1370 a         1494 c         1460 g         1304 t
ORIGIN      
        1 cctttcggtt taccggtgtc attccgctgt tatggccgcg gtttctcatt ccacgcctga
       61 cactcagttc cgtgaaggca gttcgctcca agctggactg tatgcacgaa ccccccgttc
      121 agtccgaccg ctgcgccttg tccggtaact atcaacttga gtccaacccg gaaagacacg
      181 acaaaacgcc actggcagca gccattagta acaggagtgc acgagaagat agattaagag
      241 gtcttgaagt agaggcctga ctgcggctac actgaaagaa cagttttggt aatggcggtc
      301 tcgtgaaccc tgttaccggg ttaagcagtt ccccaactga cttaaccttc gaacaaaccg
      361 cctccccagg cggtttgttc gtttacagag caagagatta cgattcattg aaaaggatct
      421 caagaagatc ctttgtagat tactatcaat taatcttccg aacatgacac cagtcactgt
      481 tgattacggt taaaccgctc cgtaacaggt ttccctcgta ttggtgactt gcagcaccaa
      541 agaagcaaaa aagataaata tatctactta ttgagagatg atatatttat ttccaaaatg
      601 ctacagatac atcatcatga aacggatcac cgcaaacagg ttactcgcct ggggattccc
      661 tttcgacccg agcatccgta tgagactcat gctcgattat tattattata gaagccccca
      721 tgaataaatc gctcatcatt ttcggcatcg tcaacataac ctcggacagt ttctccgatg
      781 gaggccggta tctggcgcca gacgcagcca ttgcgcaggc gcgtaagctg atggccgagg
      841 gggcagatgt gatcgacctc ggtccggcat ccagcaatcc cgacgccgcg cctgtttcgt
      901 ccgacacaga aatcgcgcgt atcgcgccgg tgctggacgc gctcaaggca gatggcattc
      961 ccgtctcgct cgacagttat caacccgcga cgcaagccta tgccttgtcg cgtggtgtgg
     1021 cctatctcaa tgatattcgc ggttttccag acgctgcgtt ctatccgcaa ttggcgaaat
     1081 catctgccaa actcgtcgtt atgcattcgg tgcaagacgg gcaggcagat cggcgcgagg
     1141 cacccgctgg cgacatcatg gatcacattg cggcgttctt tgacgcgcgc atcgcggcgc
     1201 tgacgggtgc cggtatcaaa cgcaaccgcc ttgtccttga tcccggcatg gggttttttc
     1261 tgggggctgc tcccgaaacc tcgctctcgg tgctggcgcg gttcgatgaa ttgcggctgc
     1321 gcttcgattt gccggtgctt ctgtctgttt cgcgcaaatc ctttctgcgc gcgctcacag
     1381 gccgtggtcc gggggatgtc ggggccgcga cactcgctgc agagcttgcc gccgccgcag
     1441 gtggagctga cttcatccgc acacacgagc cgcgcccctt gcgcgacggg ctggcggtat
     1501 tggcggcgct gaaagaaacc gcaagaattc gttaactgca cattcgggat atttctctat
     1561 attcgcgctt catcagaaaa ctgaaggaac ctccattgaa tcgaactaat attttttttg
     1621 gtgaatcgca ttctgactgg ttgcctgtca gaggcggaga atctggtgat tttgtttttc
     1681 gacgtggtga cgggcatgcc ttcgcgaaaa tcgcacctgc ttcccgccgc ggtgagctcg
     1741 ctggagagcg tgaccgcctc atttggctca aaggtcgagg tgtggcttgc cccgaggtca
     1801 tcaactggca ggaggaacag gagggtgcat gcttggtgat aacggcaatt ccgggagtac
     1861 cggcggctga tctgtctgga gcggatttgc tcaaagcgtg gccgtcaatg gggcagcaac
     1921 ttggcgctgt tcacagccta tcggttgatc aatgtccgtt tgagcgcagg ctgtcgcgaa
     1981 tgttcggacg cgccgttgat gtggtgtccc gcaatgccgt caatcccgac ttcttaccgg
     2041 acgaggacaa gagtacgccg cagctcgatc ttttggctcg tgtcgaacga gagctaccgg
     2101 tgcggctcga ccaagagcgc accgatatgg ttgtttgcca tggtgatccc tgcatgccga
     2161 acttcatggt ggaccctaaa actcttcaat gcacgggtct gatcgacctt gggcggctcg
     2221 gaacagcaga tcgctatgcc gatttggcac tcatgattgc taacgccgaa gagaactggg
     2281 cagcgccaga tgaagcagag cgcgccttcg ctgtcctatt caatgtattg gggatcgaag
     2341 cccccgaccg cgaacgcctt gccttctatc tgcgattgga ccctctgact tggggttgat
     2401 gttcatgccg cctgtttttc ctgctcattg gcacgtttcg caacctgttc tcattgcgga
     2461 caccttttcc agcctcgttt ggaaagtttc attgccagac gggactcctg caatcgtcaa
     2521 gggattgaaa cctatagaag acattgctga tgaactgcgc ggggccgact atctggtatg
     2581 gcgcaatggg aggggagcag tccggttgct cggtcgtgag aacaatctga tgttgctcga
     2641 atatgccggg gagcgaatgc tctctcacat cgttgccgag cacggcgact accaggcgac
     2701 cgaaattgca gcggaactaa tggcgaagct gtatgccgca tctgaggaac ccctgccttc
     2761 tgcccttctc ccgatccggg atcgctttgc agctttgttt cagcgggcgc gcgatgatca
     2821 aaacgcaggt tgtcaaactg actacgtcca cgcggcgatt atagccgatc aaatgatgag
     2881 caatgcctcg gaactgcgtg ggctacatgg cgatctgcat catgaaaaca tcatgttctc
     2941 cagtcgcggc tggctggtga tagatcccgt cggtctggtc ggtgaagtgg gctttggcgc
     3001 cgccaatatg ttctacgatc cggctgacag agacgacctt tgtctcgatc ctagacgcat
     3061 tgcacagatg gcggacgcat tctctcgtgc gctggacgtc gatccgcgtc gcctgctcga
     3121 ccaggcgtac gcttatgggt gcctttccgc agcttggaac gcggatggag aagaggagca
     3181 acgcgatcta gctatcgcgg ccgcgatcaa gcaggtgcga cagacgtcat actagatatc
     3241 aagcgacttc tcctatcccc tgggaacaca tcaatctcac cggagaatat cgctggccaa
     3301 agccttagcg taggattccg ccccttcccg caaacgaccc caaacaggaa acgcagctga
     3361 aacgggaagc tcaacaccca ctgacgcatg ggttgttcag gcagtacttc atcaaccagc
     3421 aaggcggcac tttcggccat ccgccgcgcc ccacagctcg ggcagaaacc gcgacgctta
     3481 cagctgaaag cgaccaggtg ctcggcgtgg caagactcgc agcgaacccg tagaaagcca
     3541 tgctccagcc gcccgcattg gagaaattct tcaaattccc gttgcacata gcccggcaat
     3601 tcctttccct gctctgccat aagcgcagcg aatgccgggt aatactcgtc aacgatctga
     3661 tagagaaggg tttgctcggg tcggtggctc tggtaacgac cagtatcccg atcccggctg
     3721 gccgtcctgg ccgccacatg aggcatgttc cgcgtccttg caatactgtg tttacataca
     3781 gtctatcgct tagcggaaag ttcttttacc ctcagccgaa atgcctgccg ttgctagaca
     3841 ttgccagcca gtgcccgtca ctcccactct tacattgttc gcagtatcta ggtacatgtt
     3901 attgcgtgca ccttcatgat gtataatgtt attaatgaca gcagaaagat cgacagaatt
     3961 aatattcttc gtacctggga cagttatcgg gtgtcggcca gtgatgagac caaaggatgc
     4021 agtataagat gaaccactac tattatcatg gccaccatta ccaccaccgt taaaactacc
     4081 tcccgaactt ccagagccgc tatgaccatc attgtagggg tcatttggtc ccatgtgacc
     4141 aaaaccaccg acagtaccgc tatttaactc tgtactcata aaattatcct ctttgacttt
     4201 taaaaacagc aaaaactgta tttataacaa tgtgtttata tacagtattc gaaatactac
     4261 gcccgtcaaa accacaccgt caacaggttt tttgtgtatt tttcacgcct cacgggcagc
     4321 ctcagacctt ttgtgttaaa acagtaatat gttctgctgt tacaatacgg tacacacagg
     4381 agacactgtc acttttatgg catggcacac agttcaagag acactgaaat taacaggaaa
     4441 gtcgcgctcg caattatatc gtgacatggc taaaggcctt gtcagctatc gcacaggaaa
     4501 aaatgatcgc cgggagttcg agaccagtga actgattcgg gcctatggcg agctgcgcac
     4561 ttctgagaca ccaaaaaggc acaaggaggg acactccaaa tctgtcgtta gccaggcgga
     4621 aaatgctcag ctagacgtca ttcagcagaa actggacgct ctgcagcaga ctgttaccct
     4681 aatgcttgaa gacaagatgg cgcgggaagc agaaaaccgc ctgcgaaatg aggagaggga
     4741 acaactacag gccgaaatta gccgtctggc tgacgcgctg gcacaggaaa aaaagcgggg
     4801 attctggtcg cggctgtttc gctccggttc agagtaacag tgcaggtgac tctgatttac
     4861 cataacatct gttatcagca ccgcctgaga ggccctcagg gcgtttctga cgggaaaccg
     4921 gtaaaacagg caacaaacca cccgaaaacc cgccagaaac gcgcaggctc tgtttttacg
     4981 gcatgcatga gtatgcgcac aagtgcatag cgcggaaatt cacaccattc agccggggta
     5041 agtcctgttt ccggcgttct tcccatccga aaacggtgaa cggggacgca atttattgcg
     5101 tggccgtgaa gcgttgccgg cgcagccgga aaacccgctg tcagcacgtt caggaagggg
     5161 gtgatgtaca aatggcacag aggatgaaag tgacgtatga gctgaaggaa tgatgtaaaa
     5221 gtgatgtata agtgatgtaa aagtgatgta tgaactgaag gagcgatgta attctgtagt
     5281 caggaagaat aacccgttca tgcaggcatg aacgatacag tgaggaacgc tttttttgtc
     5341 acgatctccc cttaacctgc gtaaatcacc cgccatggtt gattattttt caacctcaaa
     5401 atggtcatac acctaacgca gccgcgattt tcatcgcaac tgcatgaatt aaaagaaaaa
     5461 aaaggaatat ttcgttttca cgagtaatac ccgggtatta aaaatccact gtaattacat
     5521 gaaaataaag aaataaaagg taataccgga gtattaccac gtacgcgggc aagcgaaaaa
     5581 gctataacgc ttcccggccg acaggcgacc caaaatccac aggggcat
//
LOCUS       DAJTRC010000404         4410 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.guided.192, whole genome shotgun sequence.
ACCESSION   DAJTRC010000404 DAJTRC010000000
VERSION     DAJTRC010000404.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4410
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.guided.192"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..1020
                     /locus_tag="OO357_004391"
     CDS             <1..1020
                     /locus_tag="OO357_004391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA ligase RtcB family protein"
                     /protein_id="HCR5889051.1"
                     /translation="SDAVFTIASDDLWIESLAIQQLHTTANLPNMQRVVGMPDLHPGR
                     GYPIGAAFFSAGRFYPALVGNDIGCGMALWQTDILARKYNADKFEKRLSALDDVAEES
                     WLEENLPSAFAQHPWRSSLGSIGGGNHFAELQQVDQIINAELFALAGLDAQHLQLLVH
                     SGSRGLGQSILQRHIASFSHHGLPEGSDDALAFARFNRHLIALRIMQQVKATGSPVLD
                     VAHNFVSACRIGDQQGVLHRKGATPDDCGLVVIPGSRGDYSRLVQPVRSEETLHSLAH
                     GAGRKWGRTECKGRLAAKYTATQLSRTELGSRVICRDKQLIFEEAPQAYKSAESVVQC
                     QGRPD"
     gene            1082..1696
                     /locus_tag="OO357_004392"
                     /pseudo
     CDS             1082..1696
                     /locus_tag="OO357_004392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000602105.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor H"
     gene            complement(1753..3210)
                     /gene="pepD"
                     /locus_tag="OO357_004393"
     CDS             complement(1753..3210)
                     /gene="pepD"
                     /locus_tag="OO357_004393"
                     /EC_number="3.4.13.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytosol nonspecific dipeptidase"
                     /protein_id="HCR5889052.1"
                     /translation="MSELSQLSPQPLWDIFAKICSIPHPSYHEEQLAEYIVGWAKEKG
                     FHVERDQVGNILIRQPATAGMENRKPVVLQAHLDMVPQKNNDTVHDFTKDPIQPYIDG
                     EWVKARGTTLGADNGIGMASALAVLADENVVHGPLEVLLTMTEEAGMDGAFGLQSNWL
                     QADILINTDSEEEGEIYMGCAGGIDFTSNLHLDREAVPAGFETFKLTLKGLKGGHSGG
                     EIHVGLGNANKLLVRFLAGHAEELDLRLIDFNGGTLRNAIPREAFATIAVAADKVDAL
                     KSLVNTYQEILKNELAEKEKNLALLLDSVANDKAALIAKSRDTFIRLLNATPNGVIRN
                     SDVAKGVVETSLNVGVVTMTDNNVEIHCLIRSLIDSGKDYVVSMLDSLGKLAGAKTEA
                     KGAYPGWQPDANSPVMHLVRETYQRLFNKTPNIQIIHAGLECGLFKKPYPEMDMVSIG
                     PTITGPHSPDEQVHIESVGHYWTLLTELLKEIPAK"
     gene            3472..3930
                     /gene="gpt"
                     /locus_tag="OO357_004394"
     CDS             3472..3930
                     /gene="gpt"
                     /locus_tag="OO357_004394"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_308292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HCR5889053.1"
                     /translation="MSEKYIVTWDMLQIHARKLASRLMPSEQWKGIIAVSRGGLVPGA
                     LLARELGIRHVDTVCISSYDHDNQRELKVLKRAEGDGEGFIVIDDLVDTGGTAVAIRE
                     MYPKAHFVTIFAKPAGRPLVDDYVVDIPQDTWIEQPWDMGVVFVPPISGR"
     gene            4022..>4410
                     /locus_tag="OO357_004395"
     CDS             4022..>4410
                     /locus_tag="OO357_004395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_706239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HCR5889054.1"
                     /translation="MTQANLSETLFKPRFKHPETSTLVRRFNHGAQPPVQSALDGKTI
                     PHWYRMINRLMWIWRGIDPREILDVQARIVMSDAERTDDDLYDTVIGYRGGNWIYEWA
                     TQAMVWQQKACTEEDPQLSGRHWLHAAT"
BASE COUNT         1053 a         1113 c         1184 g         1060 t
ORIGIN      
        1 tccgatgcgg tatttaccat cgcatctgat gacctgtgga tcgagagttt agcgatccaa
       61 caattacaca ccacggcaaa tttacccaac atgcagcgcg tagtggggat gccagattta
      121 caccccggac gcggctaccc gattggcgca gcgttcttct ccgcaggccg tttttaccca
      181 gcactggtcg gcaatgatat cggctgtggc atggcgctat ggcaaacaga tattctcgct
      241 cgcaaataca acgccgataa gtttgaaaag cgattatcag ctttggatga cgttgctgaa
      301 gaaagctggc tggaggaaaa cctgccgtca gcatttgcac agcatccgtg gcgcagctcg
      361 cttggctcta tcggtggtgg caatcatttc gctgaactgc aacaagttga tcaaatcatc
      421 aacgctgaac tgtttgcact ggcaggtctg gatgcgcagc atctgcaact gctggttcat
      481 agcggctcga gggggctagg gcagtctatt ttacagcggc atattgcctc gttttcgcat
      541 catggtttgc ctgaaggcag tgacgacgcg ctggcgtttg cgcgttttaa tcgccacctg
      601 atcgctttgc gcataatgca acaggttaag gccaccggaa gtccggttct ggatgtggcg
      661 cataactttg ttagcgcgtg tcgaatcggt gatcaacaag gggtcttaca tcgtaaaggt
      721 gccacgcctg atgactgtgg tctggtggtt attcccggtt cacgcggtga ttactccagg
      781 ctggttcagc ccgtcaggag tgaggaaaca ttgcattcgc tggcgcatgg ggctgggcgt
      841 aaatgggggc gcaccgagtg taaagggcgt ctggcagcga aatacacagc gacgcaactc
      901 tcgcgtactg aacttggcag tcgggtaatt tgtcgcgata aacaactcat cttcgaagaa
      961 gcgccacaag cctataaatc ggctgaaagt gtggtgcaat gtcagggcag gccggattaa
     1021 ttattcctgt cgcgcggctg cgtccggtgc taacgctcaa aaacagtgga gggaaaaaag
     1081 gatgatcttg ctataactct cctcagcgca ggggccggaa gaatgttgtc tcgcagtgaa
     1141 aaaggcgctg gacaggctaa ttaaagaagc tgcccgacaa gacgtcgcgg taacggtgct
     1201 ggaaacagaa acaggccgct actctgacac gctgcgttcg gcgctgattt ctctggatgg
     1261 cgacaacgca tgggcgttaa gcgaaagctg gtgtggcact attcagtgga tttgcccgag
     1321 cccgtatcgc ccacatcacg ggcgtaaaaa ctggtttctg ggcattgggc gttttaccgc
     1381 tgatgagcag gagcaatccg atgcgatccg gtatgagacg ctgcgttcgt cggggccggg
     1441 cggtcaacat gtcaataaaa ccgactcggc ggtacgcgcc acgcatttgg catccggtat
     1501 tagcgtgaag gttcagtcag agcgcagcca gcatgctaac aaacggctgg cgcgattgct
     1561 gattgcctgg aagctggatc agcagcaata ggaaaatagc gcggtgctga aatcacagcg
     1621 gcgaatgttc catcaccaga ttgaacgtgg caacccgcga cgaacgttta cagggatggc
     1681 ttttatcgaa ggataatgaa ggatgaaact gccggaaggc gattaaacgc catccggcag
     1741 caaatcaaat aattacttcg ccggaatttc tttcagcagt tcagtcagca gtgtccagta
     1801 atgacctacg ctttcgatgt gaacttgctc atccggagag tgtggaccgg tgatagttgg
     1861 cccgatagaa accatgtcca tttccggata cggttttttg aacagaccac attccaggcc
     1921 cgcgtggata atctggatgt tcggcgtctt gttgaacagg cgctgatagg tttcacgtac
     1981 cagatgcatc accggagaat tagcgtccgg ctgccagcca ggatatgcgc ctttcgcttc
     2041 ggttttcgcg ccagccagtt tacccagcga atccagcatg ctcaccacgt agtctttacc
     2101 gctgtcgatt agtgaacgga tcaggcagtg aatttctacg ttattgtcag tcatggtcac
     2161 cacaccgacg ttcagggagg tttcgaccac accttttgcc acgtcggaat tgcggatcac
     2221 accgttcggg gtggcgttca gcagacgaat aaaggtatcg cgagatttcg caatcagggc
     2281 agctttatcg ttcgctacag agtccagcaa caaggccaga tttttctctt tctctgccag
     2341 ctcgtttttc aggatctcct gataggtatt caccagagat ttcagtgcgt cgactttatc
     2401 agctgcgaca gcaatggtcg caaaggcttc acgcgggatg gcgttacgca gtgtgccgcc
     2461 gttgaaatcg ataaggcgca ggtccagttc ttctgcatga cccgccagga agcgcaccag
     2521 cagtttgttg gcattaccca ggccaacgtg gatttcaccg ccggagtgac cgcctttcag
     2581 accttttaag gttaacttga aggtttcaaa accagctgga accgcttcac gatctaaatg
     2641 caggttggag gtgaagtcga taccccccgc acaacccatg tagatttcac cttcttcttc
     2701 ggagtcggtg ttaatcagaa tatcagcctg caaccagttg ctctgtaagc cgaacgcacc
     2761 gtccataccg gcttcttcgg tcatggtcag cagcacttcc agcgggccgt gaaccacgtt
     2821 ttcgtcagcc agaaccgcca gtgcagaggc cataccaatg ccgttatccg cacccagcgt
     2881 ggtgccgcgc gctttaaccc attcgccatc aatataaggc tggataggat ctttcgtgaa
     2941 gtcatgcacg gtgtcgttat ttttctgcgg caccatatcg aggtgggcct gtaagacgac
     3001 cggtttacga ttttccatac ctgcggtagc aggttgacga atcaggatat tacctacctg
     3061 atcgcgttcg acatggaaac ctttctcttt tgcccaacca acaatgtatt cagcgagttg
     3121 ctcttcatga taggacgggt gaggaataga acagattttg gcaaaaatat cccacagcgg
     3181 ctgtggagat aattgagaca gttcagacac gttaagtctc cttgtcgatc acccgcaaaa
     3241 cagtattgca ggtcacaggg ttagcagaaa atgttgtcaa cacaagacag gcttgcgaga
     3301 tatgtattga gaataccact ttatcccgcg tcagggagag gcagtgcgta aaaagacgcg
     3361 gactcatgtg aaatactggt ttttagtgcg ccagatctct ataatctcgc gcaacctatt
     3421 ttcccctcga acacttttta agccgtagat aaacaggctg ggacacttca catgagcgaa
     3481 aaatacatcg tcacctggga catgttgcag atccatgcac gtaaactcgc aagccgactg
     3541 atgccttctg aacaatggaa aggcattatt gccgtaagcc gtggcggtct ggtaccgggg
     3601 gcgttactgg cgcgtgaact gggtattcgt catgtcgata ccgtttgtat ttccagctac
     3661 gatcacgaca accagcgcga gcttaaagtg ctgaaacgcg cagaaggcga tggcgaaggc
     3721 ttcatcgtta ttgatgacct ggtggatacc ggtggtactg ctgttgcgat tcgtgaaatg
     3781 tatccaaaag cgcactttgt caccatcttc gcaaaaccgg ctggtcgtcc gctggttgat
     3841 gactatgttg ttgatatccc gcaagatacc tggatcgaac agccgtggga tatgggcgtc
     3901 gtattcgtcc cgccaatctc cggtcgctaa tcttttcaac gcctggcatt gccgggcgtt
     3961 gttcttttta acttcaggca ggttacaata gtttccagta agtattctgg aggctgcatc
     4021 catgacacag gcaaacctga gcgaaaccct gttcaaaccc cgctttaaac atcctgaaac
     4081 ctcgacgcta gtccgccgct ttaatcacgg cgcacaaccg cctgtgcagt cggctcttga
     4141 tggtaaaacc atccctcact ggtatcgcat gattaaccgt ctgatgtgga tctggcgcgg
     4201 tattgaccca cgtgaaatcc tcgacgtcca ggcacgtatt gtgatgagcg atgctgaacg
     4261 taccgacgat gatttatacg ataccgtgat tggctaccgt ggtggcaact ggatttatga
     4321 gtgggccacc caggcgatgg tgtggcaaca aaaagcctgt acggaagaag atccgcaact
     4381 cagtggtcga cactggctgc atgcggctac
//
LOCUS       DAJTRC010000405         4290 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.guided.194, whole genome shotgun sequence.
ACCESSION   DAJTRC010000405 DAJTRC010000000
VERSION     DAJTRC010000405.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 4290)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4290)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4290
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.guided.194"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(<1..832)
                     /locus_tag="OO357_004396"
     CDS             complement(<1..832)
                     /locus_tag="OO357_004396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4158 domain-containing protein"
                     /protein_id="HCR5889055.1"
                     /translation="MPRRSILSAAERESLLALPDSKDDLIRHYTFNDTDLSIIRQRRG
                     PANRLGFAVQLCYLRFPGVILGVDELPFPPLLKLVADQLKVGVESWNEYGQREQTRRE
                     HLSELQTVFGFRPFTMSHYRQAVQMLTELAMQTDKGIVLASALIGHLRRQSVILPALN
                     AVERASAEAITRANRRIYDALAEPLADAHRRRLDDLLKRRDNGKTTWLAWLRQSPAKP
                     NSRHMLEHIERLKAWQALDLPTGIERLVHQNRLLKIAREGGQMTPADLAKFEPQRRYA
                     T"
     gene            complement(835..1395)
                     /locus_tag="OO357_004397"
     CDS             complement(835..1395)
                     /locus_tag="OO357_004397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000147566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HCR5889056.1"
                     /translation="MTGQRIGYIRVSTFDQNPERQLEGVKVDRAFSDKASGKDVKRPQ
                     LEALISFARTGDTVVVHSMDRLARNLDDLRRIVQTLTQRGVHIEFVKEHLSFTGEDSP
                     MANLMLSVMGAFAEFERALIRERQREGIALAKQRGAYRGRKKSLSSERIAELRQRVEA
                     GEQKTKLAREFGISRETLYQYLRTDQ"
     gene            complement(1521..1871)
                     /locus_tag="OO357_004398"
     CDS             complement(1521..1871)
                     /locus_tag="OO357_004398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000454193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3330 domain-containing protein"
                     /protein_id="HCR5889057.1"
                     /translation="MEVVAEGVETPDCLAWLRQAGCDTVQGFLFARPMPAAAFVGFVN
                     QWRNTTMNANEPSTSCCVCCKEIPLDAAFTPEGAEYVEHFCGLECYQRFQARASTATE
                     TSVKPDACDSPPSG"
     gene            complement(2074..3087)
                     /gene="intI1"
                     /locus_tag="OO357_004399"
     CDS             complement(2074..3087)
                     /gene="intI1"
                     /locus_tag="OO357_004399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_005221021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1 integron integrase IntI1"
                     /protein_id="HCR5889058.1"
                     /translation="MKTATAPLPPLRSVKVLDQLRERIRYLHYSLRTEQAYVHWVRAF
                     IRFHGVRHPATLGSSEVEAFLSWLANERKVSVSTHRQALAALLFFYGKVLCTDLPWLQ
                     EIGRPRPSRRLPVVLTPDEVVRILGFLEGEHRLFAQLLYGTGMRISEGLQLRVKDLDF
                     DHGTIIVREGKGSKDRALMLPESLAPSLREQLSRARAWWLKDQAEGRSGVALPDALER
                     KYPRAGHSWPWFWVFAQHTHSTDPRSGVVRRHHMYDQTFQRAFKRAVEQAGITKPATP
                     HTLRHSFATALLRSGYDIRTVQDLLGHSDVSTTMIYTHVLKVGGAGVRSPLDALPPLT
                     SER"
     gene            3297..4127
                     /locus_tag="OO357_004400"
     CDS             3297..4127
                     /locus_tag="OO357_004400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxacillin-hydrolyzing class D beta-lactamase
                     OXA-1"
                     /protein_id="HCR5889059.1"
                     /translation="MKNTIHINFAIFLIIANIIYSSASASTDISTVASPLFEGTEGCF
                     LLYDASTNAEIAQFNKAKCATQMAPDSTFKIALSLMAFDAEIIDQKTIFKWDKTPKGM
                     EIWNSNHTPKTWMQFSVVWVSQEITQKIGLNKIKNYLKDFDYGNQDFSGDKERNNGLT
                     EAWLESSLKISPEEQIQFLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGA
                     GFTANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL
                     "
BASE COUNT         1028 a         1178 c         1174 g          910 t
ORIGIN      
        1 gagtggcgta gcgccgttgc ggctcgaatt tggccaggtc ggcgggtgtc atctggccgc
       61 cctcgcgggc aatcttgagc aggcggttct ggtgaaccag ccgctcgatg ccggtaggca
      121 gatcgagtgc ctgccatgcc ttgaggcgtt cgatgtgttc cagcatatgc cgcgaatttg
      181 gcttggccgg agactggcgc aaccaagcca accaggtcgt cttgccgttg tcccggcgct
      241 tgagcagatc gtcgaggcgg cggcgatgcg cgtccgccag tggttcggcc aaggcgtcgt
      301 agatgcgccg gttagcacgg gtgatcgcct cggcactcgc ccgctcgacg gcgttgaggg
      361 cgggcagaat gaccgactgc cgccgcaggt gcccgatcaa ggcgctggcc agcacgatgc
      421 ctttgtcggt ttgcatcgcc agctcggtca gcatctggac ggcctgccgg taatggctca
      481 tggtgaaggg ccggaaaccg aacacggttt gcagctcgct caggtgctcg cgccgggtct
      541 gctcccgctg gccgtactcg ttccagcttt cgacgccgac cttgagctgg tcggcgacca
      601 gcttcaacaa gggcgggaac ggtagttcat cgacgcccag gatgacgccg ggaaagcgca
      661 ggtaacagag ctgcaccgcg aagcccagcc gattggctgg cccgcgccgc tgtcggatga
      721 tcgagaggtc ggtatcgttg aatgtgtaat gtcggatcag gtcgtccttg gagtccggca
      781 acgccagcag gctttcccgc tcggcggcgg acaggatgga acgacgtggc atatttactg
      841 atccgttctc aagtattgat acagggtttc gcgactgatt ccgaattcac gagcaagctt
      901 ggtcttttgc tcgccagcct cgacacgttg gcgcagttcg gcaatacgct cagacgacag
      961 ggatttcttc ctgccacggt aagccccgcg ttgcttggcg agcgcaatac cctcgcgctg
     1021 acgctcgcgg atcagggcgc gctcgaactc ggcgaacgcg cccatcaccg agagcatcag
     1081 gttcgccatc ggagagtctt cgccagtaaa actgaggtgt tccttgacga attcgatatg
     1141 cacgccgcgt tgtgtcagcg tttgcacgat ccggcgcaaa tcatcgagat tgcgcgccag
     1201 gcgatccatg ctatgcacca ccacggtgtc gccggtgcgg gcgaagctta tcagcgcttc
     1261 cagttgcgga cgcttgacat ccttgccgga tgccttgtcg ctaaaagcgc gatcaacctt
     1321 gacgccttcc agttgccgtt ccgggttctg gtcgaaggtg ctgaccctga tatacccaat
     1381 gcgctgtcca gtcatggaat tccctgcaaa atgtcaggga agactctatg accttcaacg
     1441 agatatgtca ataaattcaa aattcaatcc tatcctgacg caatttacac atggcatctg
     1501 acatcaggtt agggtatgcc tcaacctgac ggcggcgaat cacaagcgtc cggtttgacg
     1561 ctggtttcgg tcgcagtgct ggcccgcgcc tggaagcgct gatagcactc cagcccgcag
     1621 aaatgctcca cgtactcggc cccttccggc gtgaaggcgg catcgagcgg gatttccttg
     1681 cagcacacgc agcaactggt gctcggttca ttggcgttca tggtggtgtt cctccattgg
     1741 ttgacgaagc cgacgaaggc cgccgccggc atcggcctgg cgaacaggaa accctgcacc
     1801 gtgtcgcaac ccgcctgccg caaccacgca aggcagtcgg gtgtttccac accctcggct
     1861 accacctcca ttgtcgtttt cagaagacgg ctgcactgaa cgtcagaagc cgactgcact
     1921 atagcagcgg aggggttgga tccatcaggc aacgacgggc tgctgccggc catcagcgga
     1981 cgcagggagg actttccgca accggccgtt cgatgcggca ccgatggcct tcgcgcaggg
     2041 gtagtgaatc cgccaggatt gacttgcgct gccctacctc tcactagtga ggggcggcag
     2101 cgcatcaagc ggtgagcgca ctccggcacc gccaactttc agcacatgcg tgtaaatcat
     2161 cgtcgtagag acgtcggaat ggccgagcag atcctgcacg gttcgaatgt cgtaaccgct
     2221 gcggagcaag gccgtcgcga acgagtggcg gagggtgtgc ggtgtggcgg gcttcgtgat
     2281 gcctgcttgt tctacggcac gtttgaaggc gcgctgaaag gtctggtcat acatgtgatg
     2341 gcgacgcacg acaccgctcc gtggatcggt cgaatgcgtg tgctgcgcaa aaacccagaa
     2401 ccacggccag gaatgcccgg cgcgcggata cttccgctca agggcgtcgg gaagcgcaac
     2461 gccgctgcgg ccctcggcct ggtccttcag ccaccatgcc cgtgcacgcg acagctgctc
     2521 gcgcaggctg ggtgccaagc tctcgggtaa catcaaggcc cgatccttgg agcccttgcc
     2581 ctcccgcacg atgatcgtgc cgtgatcgaa atccagatcc ttgacccgca gttgcaaacc
     2641 ctcactgatc cgcatgcccg ttccatacag aagctgggcg aacaaacgat gctcgccttc
     2701 cagaaaaccg aggatgcgaa ccacttcatc cggggtcagc accaccggca agcgccgcga
     2761 cggccgaggt cttccgatct cctgaagcca gggcagatcc gtgcacagca ccttgccgta
     2821 gaagaacagc aaggccgcca atgcctgacg atgcgtggag accgaaacct tgcgctcgtt
     2881 cgccagccag gacagaaatg cctcgacttc gctgctgccc aaggttgccg ggtgacgcac
     2941 accgtggaaa cggatgaagg cacgaaccca gtggacataa gcctgttcgg ttcgtaagct
     3001 gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga accttgaccg aacgcagcgg
     3061 tggtaacggc gcagtggcgg ttttcatggc ttgttatgac tgtttttttg gggtacagtc
     3121 tatgcctcgg gcatccaagc agcaagcgcg ttacgccgtg ggtcgatgtt tgatgttatg
     3181 gagcagcaac gatgttacgc agcagggcag tcgccctaaa acaaagttgg gcgaacccgg
     3241 agcctcatta attgttagcc gttaaaatta agccctttac caaaccaata cttattatga
     3301 aaaacacaat acatatcaac ttcgctattt ttttaataat tgcaaatatt atctacagca
     3361 gcgccagtgc atcaacagat atctctactg ttgcatctcc attatttgaa ggaactgaag
     3421 gttgtttttt actttacgat gcatccacaa acgctgaaat tgctcaattc aataaagcaa
     3481 agtgtgcaac gcaaatggca ccagattcaa ctttcaagat cgcattatca cttatggcat
     3541 ttgatgcgga aataatagat cagaaaacca tattcaaatg ggataaaacc cccaaaggaa
     3601 tggagatctg gaacagcaat catacaccaa agacgtggat gcaattttct gttgtttggg
     3661 tttcgcaaga aataacccaa aaaattggat taaataaaat caagaattat ctcaaagatt
     3721 ttgattatgg aaatcaagac ttctctggag ataaagaaag aaacaacgga ttaacagaag
     3781 catggctcga aagtagctta aaaatttcac cagaagaaca aattcaattc ctgcgtaaaa
     3841 ttattaatca caatctccca gttaaaaact cagccataga aaacaccata gagaacatgt
     3901 atctacaaga tctggataat agtacaaaac tgtatgggaa aactggtgca ggattcacag
     3961 caaatagaac cttacaaaac ggatggtttg aagggtttat tataagcaaa tcaggacata
     4021 aatatgtttt tgtgtccgca cttacaggaa acttggggtc gaatttaaca tcaagcataa
     4081 aagccaagaa aaatgcgatc accattctaa acacactaaa tttataaaaa atctaatggc
     4141 aaaatcgccc aacccttcaa tcaagtcggg acggccaaaa gcaagctttt ggctcccctc
     4201 gctggcgctc ggcgcccctt atttcaaacg ttaaacatca tgagggaagt ggtgatcgcc
     4261 gaagtatcga ctcaactatc agaggtagtt
//
LOCUS       DAJTRC010000406         3741 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.guided.203, whole genome shotgun sequence.
ACCESSION   DAJTRC010000406 DAJTRC010000000
VERSION     DAJTRC010000406.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.guided.203"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            263..922
                     /locus_tag="OO357_004401"
     CDS             263..922
                     /locus_tag="OO357_004401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011152976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type A-1 chloramphenicol O-acetyltransferase"
                     /protein_id="HCR5889060.1"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF
                     LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF
                     SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV
                     ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG
                     A"
     gene            complement(1123..1500)
                     /locus_tag="OO357_004402"
     CDS             complement(1123..1500)
                     /locus_tag="OO357_004402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000656306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HCR5889061.1"
                     /translation="MKDYLCKKLFNRLSGTLVIRARCGNNITGLACCNILYPSPRYSG
                     QLHIKELYVSQCDRNKGTGKAIMRFIARLALEQECLSLSWNAEKSNPGANRFYQALGG
                     RINDHIVNYYLHGESLSKLASGI"
     gene            complement(1567..>3741)
                     /locus_tag="OO357_004403"
     CDS             complement(1567..>3741)
                     /locus_tag="OO357_004403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001138064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3-like element TnAs3 family transposase"
                     /protein_id="HCR5889062.1"
                     /translation="DLAKFEPQRRYATLVALATEGMATVTDEIIDLHDRILGKLFNAA
                     KNKHQQQFQASGKAINAKVRLYGRIGQALIDAKQSGRDAFAAIEAVMSWDSFAESVTE
                     AQKLAQPDDFDFLHRIGESYATLRRYAPEFLAVLKLRAAPAAKNVLDAIEVLRGMNTD
                     NARKLPADAPTGFIKPRWQKLVMTDAGIDRRYYELCALSELKNSLRSGDIWVQGSRQF
                     KDFEDYLVPPEKFTSLKQSSELPLAVATDCEQYLHERLTLLEAQLATVNRMAAANDLP
                     DAIITESGLKITPLDAAVPDTAQALIDQTAMVLPHVKITELLLEVDEWTGFTRHFTHL
                     KSGDLAKDKNLLLTTILADAINLGLTKMAESCPGTTYAKLAWLQAWHTRDETYSTALA
                     ELVNAQFRHPFAGHWGDGTTSSSDGQNFRTASKAKSTGHINPKYGSSPGRTFYTHISD
                     QYAPFHTKVVNVGLRDSTYVLDGLLYHESDLRIEEHYTDTAGFTDHVFALMHLLGFRF
                     APRIRDLGDTKLYIPKGDAAYDALKPMIGGTLNIKHVRAHWDEILRLATSIKQGTVTA
                     SLMLRKLGSYPRQNGLAVALRELGRIERTLFILDWLQSVELRRRVHAGLNKGEARNAL
                     ARAVFFNRLGEIRDRSFEQQRYRASGLNLVTAAIVLWNTVYLERAAHALRGNGHAVDD
                     SLLQYLSPLGWEHINLTGDYLWRSSAKIGAGKFRPLRPLQPA"
BASE COUNT          784 a          986 c         1100 g          871 t
ORIGIN      
        1 caccatcata cactaaatca gtaagttggc agcatcaccc gacgcacttt gcgccgaata
       61 aatacctgtg acggaagatc acttcgcaga ataaataaat cctggtgtcc ctgttgatac
      121 cgggaagccc tgggccaact tttggcgaaa atgagacgtt gatcggcacg taagaggttc
      181 caactttcac cataatgaaa taagatcact accgggcgta ttttttgagt tatcgagatt
      241 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
      301 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
      361 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
      421 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
      481 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
      541 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
      601 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
      661 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
      721 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
      781 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
      841 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
      901 tgagtggcag ggcggggcgt aattttttta aggcagttat tggtgccctt aaacgcctgg
      961 ttgctacgcc tgaataagtg ataataagcg gatgaatggc agaaattcga aagcaaattc
     1021 gacccggtcg tcggttcagg gcagggtcgt taaatagccg cttatgtcta ttgctggttt
     1081 accggtttat tgactaccgg aagcagtgtg accgtgtgct tctcaaatgc ctgaggccag
     1141 tttgctcagg ctctccccgt ggaggtaata attgacgata tgatcattta ttctgcctcc
     1201 cagagcctga taaaaacggt tagcgccggg gttggatttt tcagcgttcc agctaaggct
     1261 aaggcattcc tgttcaagcg caagccgggc tataaagcgc attatcgctt tacccgtgcc
     1321 cttatttcga tcgcactgag aaacatacag ctctttaata tgcagctgac cgctgtatcg
     1381 gggcgaggga taaagaatat tgcaacatgc caggccagta atgttattgc cgcagcgcgc
     1441 tctgatcacc agggtaccgg aaagccggtt gaataacttt ttacagagat aatccttcat
     1501 caacgcttcc tgaatgatac cttcgccata ataggggtcg tctcagaaaa cggaaaataa
     1561 agcacgctaa gccggttgca gaggccgtag cggcctgaac ttccccgcgc cgatcttggc
     1621 gctgctgcgc cataggtaat caccggtcag gttgatgtgc tcccagccga gtggcgacag
     1681 gtactgcaat agcgagtcat cgacggcatg accattgccg cgcaacgcat gcgccgcacg
     1741 ctccaggtag accgtgttcc acagcacgat ggccgccgtc accaggttga ggccgctggc
     1801 ccggtagcgc tgctgctcga aactgcggtc acggatttca ccaaggcggt tgaagaacac
     1861 ggcacgggcc agcgcattgc gcgcctcgcc cttgttcagc ccggcatgca cgcggcggcg
     1921 tagctcgacg ctttgcagcc agtcgaggat gaacagcgtg cgctcgatgc ggcccaactc
     1981 gcgcagcgcg acggccaagc cgttctggcg cgggtagctg ccgagtttcc tgagcatcag
     2041 cgaggccgtc accgtgccct gcttgatcga ggtggccagc cgcaggattt cgtcccaatg
     2101 ggcgcggacg tgcttgatgt tgagcgtgcc gccgatcatc ggcttgagcg cgtcataggc
     2161 ggcatcgccc ttcgggatgt agagcttggt gtcgcccagg tcgcggatgc gcggcgcgaa
     2221 gcggaagccc aagaggtgca tcagggcgaa gacgtgatcg gtgaagcccg ccgtgtcggt
     2281 gtagtgctcc tcgatccgca ggtcggattc gtggtacagc aggccgtcga gcacgtaggt
     2341 tgagtcgcgc aggccgacat tgaccacctt ggtgtggaat ggcgcgtatt ggtcggagat
     2401 gtgggtgtag aaagtccgtc ctgggctgct gccatatttt gggttgatgt gccccgtgct
     2461 ctttgccttg ctagcggttc ggaaattctg tccgtccgat gatgatgtgg tgccatcgcc
     2521 ccagtgcccg gcaaagggat gccgaaactg agcgttgacc agttcagcca acgctgtcga
     2581 gtacgtttcg tcgcgggtat gccaggcttg cagccaagcg agcttcgcgt aggtcgtgcc
     2641 ggggcaggac tcggccatct tggtcaggcc caggttgatc gcgtcggcca ggatcgtggt
     2701 caacaacagg ttcttgtcct tggccagatc gcccgatttc aagtgcgtga agtgccgggt
     2761 gaagcccgtc cactcatcga cttcgagcag cagttcggtg atcttgacgt gcggcaggac
     2821 catggctgtc tggtctatca gcgcctgcgc ggtgtcgggc accgccgcat ccagcggcgt
     2881 gatcttcaag cccgactcgg tgatgatggc atccggcagg tcgttggctg ccgccatgcg
     2941 gttgacggtg gcaagttgtg cttccagcag cgtcagccgc tcatgcagat attgttcgca
     3001 gtcggtggcc acggccagcg gcaattcgct ggactgcttg aggctggtga acttctcggg
     3061 cggtaccagg tagtcctcga agtccttgaa ctggcgtgaa ccctgcaccc agatgtcgcc
     3121 cgagcgcagg gagttcttca actcggacag cgcgcacagt tcgtagtagc gccggtcgat
     3181 gccggcgtcg gtcatcacca gtttctgcca gcgcggcttg atgaagccgg tcggtgcatc
     3241 ggctggcagc ttgcgggcgt tgtcggtgtt catgccgcgc agcacctcaa tggcatcaag
     3301 cacgtttttg gcggcgggcg cggcccgcag cttgagcacg gcaaggaatt ccggtgcata
     3361 gcggcgcagg gtggcgtagc tctcgccgat gcgatgcagg aaatcgaagt catcgggttg
     3421 cgcgagcttc tgcgcctcgg tgacgctctc ggcaaaggaa tcccaggaca tgacggcctc
     3481 gatggcggca aacgcatcgc ggcctgattg cttggcgtcg atcagcgcct gaccgatgcg
     3541 cccgtacaga cgtaccttgg cgttgatggc cttgcctgac gcctggaact gctgctgatg
     3601 cttattcttg gcagcgttaa acagcttacc caggatgcgg tcgtgcaggt cgatgatttc
     3661 gtcggtgacg gtggccatgc cctcggtggc cagcgccacg agagtggcgt agcgccgttg
     3721 cggctcgaat ttggccaggt c
//
LOCUS       DAJTRC010000407         3302 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.guided.211, whole genome shotgun sequence.
ACCESSION   DAJTRC010000407 DAJTRC010000000
VERSION     DAJTRC010000407.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3302)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3302)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3302
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.guided.211"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            40..162
                     /locus_tag="OO357_004404"
                     /pseudo
     CDS             40..162
                     /locus_tag="OO357_004404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001284954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
     gene            complement(140..763)
                     /gene="tetR(B)"
                     /locus_tag="OO357_004405"
     CDS             complement(140..763)
                     /gene="tetR(B)"
                     /locus_tag="OO357_004405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000088605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance transcriptional
                     repressor TetR(B)"
                     /protein_id="HCR5889063.1"
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWH
                     VKNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLG
                     TRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERET
                     PTTDSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     gene            845..2050
                     /gene="tet(B)"
                     /locus_tag="OO357_004406"
     CDS             845..2050
                     /gene="tet(B)"
                     /locus_tag="OO357_004406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001089064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(B)"
                     /protein_id="HCR5889064.1"
                     /translation="MNSSTKIALVITLLDAMGIGLIMPVLPTLLREFIASEDIANHFG
                     VLLALYALMQVIFAPWLGKMSDRFGRRPVLLLSLIGASLDYLLLAFSSALWMLYLGRL
                     LSGITGATGAVAASVIADTTSASQRVKWFGWLGASFGLGLIAGPIIGGFAGEISPHSP
                     FFIAALLNIVAFLVVMFWFRETKNTRDNTDTEVGVETQSNSVYITLFKTMPILLIIYF
                     SAQLIGQIPATVWVLFTENRFGWNSMMVGFSLAGLGLLHSVFQAFVAGRIATKWGEKT
                     AVLLGFIADSSAFAFLAFISEGWLVFPVLILLAGGGIALPALQGVMSIQTKSHQQGAL
                     QGLLVSLTNATGVIGPLLFAVIYNHSLPIWDGWIWIIGLAFYCIIILLSMTFMLTPQA
                     QGSKQETSA"
     gene            complement(2163..2756)
                     /gene="tetC"
                     /locus_tag="OO357_004407"
     CDS             complement(2163..2756)
                     /gene="tetC"
                     /locus_tag="OO357_004407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000428546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracyline resistance-associated
                     transcriptional repressor TetC"
                     /protein_id="HCR5889065.1"
                     /translation="MENKNHQQENFKSTYQSLVNSARILFVEKGYQAVSIDEISGKAL
                     VTKGAFYHHFKNKKQLLSACYKQQLIMIDAYITTKTDLTNGWSALESIFEHYLDYIID
                     NNKNLIPIQEVMPIIGWNELEKISLEYITGKVNAIVSKLIQENQLKAYDDDVLKNLLN
                     GWFMHIAIHAKNLKELADKKGQFIAIYRGFLLSLKDK"
     gene            2889..3260
                     /locus_tag="OO357_004408"
     CDS             2889..3260
                     /locus_tag="OO357_004408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HCR5889066.1"
                     /translation="MQLRYDDKQFHTTVIKDVLLWIEHNLDQSLLLDDVANKAGYTKW
                     YFQRLFKKVTGVTLASYIRARRLTKAAVELRLTKKTILEIALKYQFDSQQSFTRRFKY
                     IFKVTPSYYRRNKLWELEAMH"
BASE COUNT          899 a          602 c          677 g         1124 t
ORIGIN      
        1 tgaacataaa acactatcaa taagttggag tcattacccg ttggagccgc attattttcg
       61 ctttatgaat ctaaagggtg gttaactcga catcttggtt accgtgaagt taccatcacg
      121 gaaaaaggtt atgctgcttt taagacccac tttcacattt aagttgtttt tctaatccgc
      181 atatgatcaa ttcaaggccg aataagaagg ctggctctgc accttggtga tcaaataatt
      241 cgatagcttg tcgtaataat ggcggcatac tatcagtagt aggtgtttcc ctttcttctt
      301 tagcgacttg atgctcttga tcttccaata cgcaacctaa agtaaaatgc cccacagcgc
      361 tgagtgcata taatgcattc tctagtgaaa aaccttgttg gcataaaaag gctaattgat
      421 tttcgagagt ttcatactgt ttttctgtag gccgtgtacc taaatgtact tttgctccat
      481 cgcgatgact tagtaaagca catctaaaac ttttagcgtt attacgtaaa aaatcttgcc
      541 agctttcccc ttctaaaggg caaaagtgag tatggtgcct atctaacatc tcaatggcta
      601 aggcgtcgag caaagcccgc ttatttttta catgccaata caatgtaggc tgctctacac
      661 ctagcttctg ggcgagttta cgggttgtta aaccttcgat tccgacctca ttaagcagct
      721 ctaatgcgct gttaatcact ttacttttat ctaatctaga catcattaat tcctaatttt
      781 tgttgacact ctatcattga tagagttatt ttaccactcc ctatcagtga tagagaaaag
      841 tgaaatgaat agttcgacaa agatcgcatt ggtaattacg ttactcgatg ccatggggat
      901 tggccttatc atgccagtct tgccaacgtt attacgtgaa tttattgctt cggaagatat
      961 cgctaaccac tttggcgtat tgcttgcact ttatgcgtta atgcaggtta tctttgctcc
     1021 ttggcttgga aaaatgtctg accgatttgg tcggcgccca gtgctgttgt tgtcattaat
     1081 aggcgcatcg ctggattact tattgctggc tttttcaagt gcgctttgga tgctgtattt
     1141 aggccgtttg ctttcaggga tcacaggagc tactggggct gtcgcggcat cggtcattgc
     1201 cgataccacc tcagcttctc aacgcgtgaa gtggttcggt tggttagggg caagttttgg
     1261 gcttggttta atagcggggc ctattattgg tggttttgca ggagagattt caccgcatag
     1321 tccctttttt atcgctgcgt tgctaaatat tgtcgctttc cttgtggtta tgttttggtt
     1381 ccgtgaaacc aaaaatacac gtgataatac agataccgaa gtaggggttg agacgcaatc
     1441 gaattcggta tacatcactt tatttaaaac gatgcccatt ttgttgatta tttatttttc
     1501 agcgcaattg ataggccaaa ttcccgcaac ggtgtgggtg ctatttaccg aaaatcgttt
     1561 tggatggaat agcatgatgg ttggcttttc attagcgggt cttggtcttt tacactcagt
     1621 attccaagcc tttgtggcag gaagaatagc cactaaatgg ggcgaaaaaa cggcagtact
     1681 gctcggattt attgcagata gtagtgcatt tgccttttta gcgtttatat ctgaaggttg
     1741 gttagttttc cctgttttaa ttttattggc tggtggtggg atcgctttac ctgcattaca
     1801 gggagtgatg tctatccaaa caaagagtca tcagcaaggt gctttacagg gattattggt
     1861 gagccttacc aatgcaaccg gtgttattgg cccattactg tttgctgtta tttataatca
     1921 ttcactacca atttgggatg gctggatttg gattattggt ttagcgtttt actgtattat
     1981 tatcctgcta tcgatgacct tcatgttaac ccctcaagct caggggagta aacaggagac
     2041 aagtgcttag ttatttcgtc accaaatgat gttattccgc gaaatataat gaccctcttg
     2101 ataacccaag agggcatttt ttacgataaa gaagatttag cttcaaataa aacctatcta
     2161 ttttatttat ctttcaagct caataaaaag ccgcggtaaa tagcaataaa ttggcctttt
     2221 ttatcggcaa gctcttttag gtttttcgca tgtattgcga tatgcataaa ccagccattg
     2281 agtaagtttt taagcacatc atcatcataa gctttaagtt ggttctcttg gatcaatttg
     2341 ctgacaatgg cgtttacctt accagtaatg tattcaaggc taattttttc aagttcattc
     2401 caaccaatga taggcatcac ttcttggata gggataaggt ttttattatt atcaataata
     2461 taatcaagat aatgttcaaa tatactttct aaggcagacc aaccatttgt taaatcagtt
     2521 tttgttgtga tgtaggcatc aatcataatt aattgctgct tataacaggc actgagtaat
     2581 tgttttttat ttttaaagtg atgataaaag gcacctttgg tcaccaacgc ttttcccgag
     2641 atctcatcta ttgaaacagc ttgatagcct ttttcaacaa acaatattcg tgctgagtta
     2701 accagtgatt gataggtact cttaaaattt tcttgttgat gatttttatt ttccatgata
     2761 gatttaaaat aacataccgt cagtatgttt atggtatcat gatgatgtgg tcgtgacaat
     2821 cttaagaaca tttaggttat tttatgtata ttgaacagca ttctcgctat caaaataaag
     2881 ctaataacat ccaattaaga tatgatgata agcagtttca tacaacggtt atcaaagatg
     2941 ttctattatg gattgaacat aatttagatc agtctttact gcttgatgat gtggcgaata
     3001 aagcgggtta taccaagtgg tattttcagc ggctgttcaa aaaagtaaca ggggtcacac
     3061 tggctagcta tattcgtgct cgtcgtttga cgaaagcggc tgttgagttg aggttgacga
     3121 aaaaaactat ccttgagatc gcattaaaat atcaatttga ttcccaacaa tcttttacac
     3181 gtcgatttaa gtacattttt aaggttacac caagttatta tcggcgtaat aaattatggg
     3241 aattggaggc aatgcactga gagggtgatg ctgccaactt actgatttag tgtatgatgg
     3301 tg
//
LOCUS       DAJTRC010000408         3133 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.guided.217, whole genome shotgun sequence.
ACCESSION   DAJTRC010000408 DAJTRC010000000
VERSION     DAJTRC010000408.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.guided.217"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(1..800)
                     /gene="intI2"
                     /locus_tag="OO357_004409"
                     /pseudo
     CDS             complement(1..800)
                     /gene="intI2"
                     /locus_tag="OO357_004409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            1132..1605
                     /locus_tag="OO357_004410"
     CDS             1132..1605
                     /locus_tag="OO357_004410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5889067.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1700..2224
                     /gene="sat2"
                     /locus_tag="OO357_004411"
     CDS             1700..2224
                     /gene="sat2"
                     /locus_tag="OO357_004411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5889068.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            2282..3073
                     /gene="aadA1"
                     /locus_tag="OO357_004412"
                     /pseudo
     CDS             2282..3073
                     /gene="aadA1"
                     /locus_tag="OO357_004412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          866 a          696 c          759 g          812 t
ORIGIN      
        1 gtatgacaag tgacacgctt gctaacgatg cctgcttttt gtacggctgc cttcaatgcc
       61 tttcgcgcaa cggagtcatg cagatgatgg cggcataatt tgccgttata cgggtggttg
      121 cagagcgtgc tggagggaaa gacaaacatc cacgccgctt gtcgataagc agaagggtat
      181 ttgtgatcta aagcaaaagg cagcgatggc cctacgcctt gtaagttgtc gtcttgctga
      241 ataagccgcg cttgctcaat gagttatttt attgctggga ttaggcgcgt gggcagtagg
      301 ctgtttctgc ttttcccacc cttaccgtca tgcacagtga tgcagccatt atcaaaatca
      361 aaatctttaa cccgcaaacg caagcattca ttaatgcgca aacctgcacc atacagcagc
      421 gtaaaaataa cttggttgcg agtatccata acctgcaaaa tgcgttgcac ttcatttgca
      481 gagataacag agggtagccg tctaggcttg cttgcaggga tataatcaat atcgcccaac
      541 ggctgttgta aaaacctgtt gtacaaaaaa gctagggcat ttaaagcgat tttctgcgtg
      601 tttatggcta catgtctgct gtttgctaag ctggataaaa acagcctgac ctcttcactg
      661 cccatggtct gaggatgacg ttttttgtga aacagaataa aacgcttaat ccagtgcagg
      721 taagtttttt cagttttcag cgcataacct ttttgtcgca tatccgtgcg tatagaattt
      781 aaaaatggac tgttagacat aaaacgctcc ttgtcttgca actgtctgcc tatacagcct
      841 attctagctg ggatttaaaa aagtgcctgt tttttacgcc tagagatgct tgtttaccgg
      901 tagagtttta atttaatgct aaataaatta aaatgttatg agttctttgg gtgagataat
      961 gtgcatcgtg caagcaggat agacggcatg cacgatttgt aataacagag tgtcttgtat
     1021 ttttaaagaa agtctattta atacaagtga ttatattaat taacggtaag catcagcggg
     1081 tgacaaaacg agcatgctta ctaataaaat gttaacctct gaggaagaat tgtgaaacta
     1141 tcactaatgg tagctatatc gaagaatgga gttatcggga atggccctga tattccatgg
     1201 agtgccaaag gtgaacagct cctgtttaaa gctattacct ataaccaatg gctgttggtt
     1261 ggacgcaaga cttttgaatc aatgggagca ttacccaacc gaaagtatgc ggtcgtaaca
     1321 cgttcaagtt ttacatctga caatgagaac gtattgatct ttccatcaat taaagatgct
     1381 ttaaccaacc taaagaaaat aacggatcat gtcattgttt caggtggtgg ggagatatac
     1441 aaaagcctga tcgatcaagt agatacacta catatatcta caatagacat cgagccggaa
     1501 ggtgatgttt actttcctga aatccccagc aattttaggc cagtttttac ccaagacttc
     1561 gcctctaaca taaattatag ttaccaaatc tggcaaaagg gttaacaagt ggcagcaacg
     1621 gattcgcaaa cctgtcacgc cttttgtacc aaaagccgcg ccaggtttgc gatccgctgt
     1681 gccaggcgtt aggcgtcata tgaagatttc ggtgatccct gagcaggtgg cggaaacatt
     1741 ggatgctgag aaccatttca ttgttcgtga agtgttcgat gtgcacctat ccgaccaagg
     1801 ctttgaacta tctaccagaa gtgtgagccc ctaccggaag gattacatct cggatgatga
     1861 ctctgatgaa gactctgctt gctatggcgc attcatcgac caagagcttg tcgggaagat
     1921 tgaactcaac tcaacatgga acgatctagc ctctatcgaa cacattgttg tgtcgcacac
     1981 gcaccgaggc aaaggagtcg cgcacagtct catcgaattt gcgaaaaagt gggcactaag
     2041 cagacagctc cttggcatac gattagagac acaaacgaac aatgtacctg cctgcaattt
     2101 gtacgcaaaa tgtggcttta ctctcggcgg cattgacctg ttcacgtata aaactagacc
     2161 tcaagtctcg aacgaaacag cgatgtactg gtactggttc tcgggagcac aggatgacgc
     2221 ctaacaattc attcaagccg acaccgcttc gcggcgcggc ttaattcagg agttaaacat
     2281 catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     2341 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     2401 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     2461 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     2521 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     2581 ttaaccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     2641 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     2701 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     2761 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     2821 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     2881 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     2941 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     3001 ctcgcgcgca gatcagttgg aagaatttgt tcactacgtg aaaggcgaga tcaccaaggt
     3061 agtcggcaaa taatgtctaa caattcgttc aagccgacgc cgcttcgcgg cgcggcttaa
     3121 ctcaagcgtt aga
//
LOCUS       DAJTRC010000409         3133 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.guided.218, whole genome shotgun sequence.
ACCESSION   DAJTRC010000409 DAJTRC010000000
VERSION     DAJTRC010000409.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3133
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.guided.218"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(1..800)
                     /gene="intI2"
                     /locus_tag="OO357_004413"
                     /pseudo
     CDS             complement(1..800)
                     /gene="intI2"
                     /locus_tag="OO357_004413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            1132..1605
                     /locus_tag="OO357_004414"
     CDS             1132..1605
                     /locus_tag="OO357_004414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5889069.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1700..2224
                     /gene="sat2"
                     /locus_tag="OO357_004415"
     CDS             1700..2224
                     /gene="sat2"
                     /locus_tag="OO357_004415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5889070.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            2282..3073
                     /gene="aadA1"
                     /locus_tag="OO357_004416"
     CDS             2282..3073
                     /gene="aadA1"
                     /locus_tag="OO357_004416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5889071.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          865 a          696 c          759 g          813 t
ORIGIN      
        1 gtatgacaag tgacacgctt gctaacgatg cctgcttttt gtacggctgc cttcaatgcc
       61 tttcgcgcaa cggagtcatg cagatgatgg cggcataatt tgccgttata cgggtggttg
      121 cagagcgtgc tggagggaaa gacaaacatc cacgccgctt gtcgataagc agaagggtat
      181 ttgtgatcta aagcaaaagg cagcgatggc cctacgcctt gtaagttgtc gtcttgctga
      241 ataagccgcg cttgctcaat gagttatttt attgctggga ttaggcgcgt gggcagtagg
      301 ctgtttctgc ttttcccacc cttaccgtca tgcacagtga tgcagccatt atcaaaatca
      361 aaatctttaa cccgcaaacg caagcattca ttaatgcgca aacctgcacc atacagcagc
      421 gtaaaaataa cttggttgcg agtatccata acctgcaaaa tgcgttgcac ttcatttgca
      481 gagataacag agggtagccg tctaggcttg cttgcaggga tataatcaat atcgcccaac
      541 ggctgttgta aaaacctgtt gtacaaaaaa gctagggcat ttaaagcgat tttctgcgtg
      601 tttatggcta catgtctgct gtttgctaag ctggataaaa acagcctgac ctcttcactg
      661 cccatggtct gaggatgacg ttttttgtga aacagaataa aacgcttaat ccagtgcagg
      721 taagtttttt cagttttcag cgcataacct ttttgtcgca tatccgtgcg tatagaattt
      781 aaaaatggac tgttagacat aaaacgctcc ttgtcttgca actgtctgcc tatacagcct
      841 attctagctg ggatttaaaa aagtgcctgt tttttacgcc tagagatgct tgtttaccgg
      901 tagagtttta atttaatgct aaataaatta aaatgttatg agttctttgg gtgagataat
      961 gtgcatcgtg caagcaggat agacggcatg cacgatttgt aataacagag tgtcttgtat
     1021 ttttaaagaa agtctattta atacaagtga ttatattaat taacggtaag catcagcggg
     1081 tgacaaaacg agcatgctta ctaataaaat gttaacctct gaggaagaat tgtgaaacta
     1141 tcactaatgg tagctatatc gaagaatgga gttatcggga atggccctga tattccatgg
     1201 agtgccaaag gtgaacagct cctgtttaaa gctattacct ataaccaatg gctgttggtt
     1261 ggacgcaaga cttttgaatc aatgggagca ttacccaacc gaaagtatgc ggtcgtaaca
     1321 cgttcaagtt ttacatctga caatgagaac gtattgatct ttccatcaat taaagatgct
     1381 ttaaccaacc taaagaaaat aacggatcat gtcattgttt caggtggtgg ggagatatac
     1441 aaaagcctga tcgatcaagt agatacacta catatatcta caatagacat cgagccggaa
     1501 ggtgatgttt actttcctga aatccccagc aattttaggc cagtttttac ccaagacttc
     1561 gcctctaaca taaattatag ttaccaaatc tggcaaaagg gttaacaagt ggcagcaacg
     1621 gattcgcaaa cctgtcacgc cttttgtacc aaaagccgcg ccaggtttgc gatccgctgt
     1681 gccaggcgtt aggcgtcata tgaagatttc ggtgatccct gagcaggtgg cggaaacatt
     1741 ggatgctgag aaccatttca ttgttcgtga agtgttcgat gtgcacctat ccgaccaagg
     1801 ctttgaacta tctaccagaa gtgtgagccc ctaccggaag gattacatct cggatgatga
     1861 ctctgatgaa gactctgctt gctatggcgc attcatcgac caagagcttg tcgggaagat
     1921 tgaactcaac tcaacatgga acgatctagc ctctatcgaa cacattgttg tgtcgcacac
     1981 gcaccgaggc aaaggagtcg cgcacagtct catcgaattt gcgaaaaagt gggcactaag
     2041 cagacagctc cttggcatac gattagagac acaaacgaac aatgtacctg cctgcaattt
     2101 gtacgcaaaa tgtggcttta ctctcggcgg cattgacctg ttcacgtata aaactagacc
     2161 tcaagtctcg aacgaaacag cgatgtactg gtactggttc tcgggagcac aggatgacgc
     2221 ctaacaattc attcaagccg acaccgcttc gcggcgcggc ttaattcagg agttaaacat
     2281 catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     2341 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     2401 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     2461 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     2521 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     2581 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     2641 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     2701 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     2761 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     2821 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     2881 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     2941 tcagcccgtc atacttgaag ctagacaggc ttatcttgga caagaagaag atcgcttggc
     3001 ctcgcgcgca gatcagttgg aagaatttgt tcactacgtg aaaggcgaga tcaccaaggt
     3061 agtcggcaaa taatgtctaa caattcgttc aagccgacgc cgcttcgcgg cgcggcttaa
     3121 ctcaagcgtt aga
//
LOCUS       DAJTRC010000410         3130 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.guided.219, whole genome shotgun sequence.
ACCESSION   DAJTRC010000410 DAJTRC010000000
VERSION     DAJTRC010000410.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.guided.219"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(1..800)
                     /gene="intI2"
                     /locus_tag="OO357_004417"
                     /pseudo
     CDS             complement(1..800)
                     /gene="intI2"
                     /locus_tag="OO357_004417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            1132..1605
                     /locus_tag="OO357_004418"
     CDS             1132..1605
                     /locus_tag="OO357_004418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5889072.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1700..2224
                     /gene="sat2"
                     /locus_tag="OO357_004419"
     CDS             1700..2224
                     /gene="sat2"
                     /locus_tag="OO357_004419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5889073.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            2282..3070
                     /locus_tag="OO357_004420"
                     /pseudo
     CDS             2282..3070
                     /locus_tag="OO357_004420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
BASE COUNT          863 a          696 c          759 g          812 t
ORIGIN      
        1 gtatgacaag tgacacgctt gctaacgatg cctgcttttt gtacggctgc cttcaatgcc
       61 tttcgcgcaa cggagtcatg cagatgatgg cggcataatt tgccgttata cgggtggttg
      121 cagagcgtgc tggagggaaa gacaaacatc cacgccgctt gtcgataagc agaagggtat
      181 ttgtgatcta aagcaaaagg cagcgatggc cctacgcctt gtaagttgtc gtcttgctga
      241 ataagccgcg cttgctcaat gagttatttt attgctggga ttaggcgcgt gggcagtagg
      301 ctgtttctgc ttttcccacc cttaccgtca tgcacagtga tgcagccatt atcaaaatca
      361 aaatctttaa cccgcaaacg caagcattca ttaatgcgca aacctgcacc atacagcagc
      421 gtaaaaataa cttggttgcg agtatccata acctgcaaaa tgcgttgcac ttcatttgca
      481 gagataacag agggtagccg tctaggcttg cttgcaggga tataatcaat atcgcccaac
      541 ggctgttgta aaaacctgtt gtacaaaaaa gctagggcat ttaaagcgat tttctgcgtg
      601 tttatggcta catgtctgct gtttgctaag ctggataaaa acagcctgac ctcttcactg
      661 cccatggtct gaggatgacg ttttttgtga aacagaataa aacgcttaat ccagtgcagg
      721 taagtttttt cagttttcag cgcataacct ttttgtcgca tatccgtgcg tatagaattt
      781 aaaaatggac tgttagacat aaaacgctcc ttgtcttgca actgtctgcc tatacagcct
      841 attctagctg ggatttaaaa aagtgcctgt tttttacgcc tagagatgct tgtttaccgg
      901 tagagtttta atttaatgct aaataaatta aaatgttatg agttctttgg gtgagataat
      961 gtgcatcgtg caagcaggat agacggcatg cacgatttgt aataacagag tgtcttgtat
     1021 ttttaaagaa agtctattta atacaagtga ttatattaat taacggtaag catcagcggg
     1081 tgacaaaacg agcatgctta ctaataaaat gttaacctct gaggaagaat tgtgaaacta
     1141 tcactaatgg tagctatatc gaagaatgga gttatcggga atggccctga tattccatgg
     1201 agtgccaaag gtgaacagct cctgtttaaa gctattacct ataaccaatg gctgttggtt
     1261 ggacgcaaga cttttgaatc aatgggagca ttacccaacc gaaagtatgc ggtcgtaaca
     1321 cgttcaagtt ttacatctga caatgagaac gtattgatct ttccatcaat taaagatgct
     1381 ttaaccaacc taaagaaaat aacggatcat gtcattgttt caggtggtgg ggagatatac
     1441 aaaagcctga tcgatcaagt agatacacta catatatcta caatagacat cgagccggaa
     1501 ggtgatgttt actttcctga aatccccagc aattttaggc cagtttttac ccaagacttc
     1561 gcctctaaca taaattatag ttaccaaatc tggcaaaagg gttaacaagt ggcagcaacg
     1621 gattcgcaaa cctgtcacgc cttttgtacc aaaagccgcg ccaggtttgc gatccgctgt
     1681 gccaggcgtt aggcgtcata tgaagatttc ggtgatccct gagcaggtgg cggaaacatt
     1741 ggatgctgag aaccatttca ttgttcgtga agtgttcgat gtgcacctat ccgaccaagg
     1801 ctttgaacta tctaccagaa gtgtgagccc ctaccggaag gattacatct cggatgatga
     1861 ctctgatgaa gactctgctt gctatggcgc attcatcgac caagagcttg tcgggaagat
     1921 tgaactcaac tcaacatgga acgatctagc ctctatcgaa cacattgttg tgtcgcacac
     1981 gcaccgaggc aaaggagtcg cgcacagtct catcgaattt gcgaaaaagt gggcactaag
     2041 cagacagctc cttggcatac gattagagac acaaacgaac aatgtacctg cctgcaattt
     2101 gtacgcaaaa tgtggcttta ctctcggcgg cattgacctg ttcacgtata aaactagacc
     2161 tcaagtctcg aacgaaacag cgatgtactg gtactggttc tcgggagcac aggatgacgc
     2221 ctaacaattc attcaagccg acaccgcttc gcggcgcggc ttaattcagg agttaaacat
     2281 catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     2341 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     2401 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     2461 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     2521 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     2581 ttaaccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     2641 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     2701 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     2761 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     2821 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     2881 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     2941 tcagcccgtc atacttgaag ctaggcaggc ttatcttgga caagaagatc gcttggcctc
     3001 gcgcgcagat cagttggaag aatttgttca ctacgtgaaa ggcgagatca ccaaggtagt
     3061 cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc
     3121 aagcgttaga
//
LOCUS       DAJTRC010000411         3130 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.guided.220, whole genome shotgun sequence.
ACCESSION   DAJTRC010000411 DAJTRC010000000
VERSION     DAJTRC010000411.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 3130)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3130)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3130
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.guided.220"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            complement(1..800)
                     /gene="intI2"
                     /locus_tag="OO357_004421"
                     /pseudo
     CDS             complement(1..800)
                     /gene="intI2"
                     /locus_tag="OO357_004421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_023994230.1"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="class 2 integron integrase IntI2"
     gene            1132..1605
                     /locus_tag="OO357_004422"
     CDS             1132..1605
                     /locus_tag="OO357_004422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001355915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrA1"
                     /protein_id="HCR5889074.1"
                     /translation="MKLSLMVAISKNGVIGNGPDIPWSAKGEQLLFKAITYNQWLLVG
                     RKTFESMGALPNRKYAVVTRSSFTSDNENVLIFPSIKDALTNLKKITDHVIVSGGGEI
                     YKSLIDQVDTLHISTIDIEPEGDVYFPEIPSNFRPVFTQDFASNINYSYQIWQKG"
     gene            1700..2224
                     /gene="sat2"
                     /locus_tag="OO357_004423"
     CDS             1700..2224
                     /gene="sat2"
                     /locus_tag="OO357_004423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000704156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat2"
                     /protein_id="HCR5889075.1"
                     /translation="MKISVIPEQVAETLDAENHFIVREVFDVHLSDQGFELSTRSVSP
                     YRKDYISDDDSDEDSACYGAFIDQELVGKIELNSTWNDLASIEHIVVSHTHRGKGVAH
                     SLIEFAKKWALSRQLLGIRLETQTNNVPACNLYAKCGFTLGGIDLFTYKTRPQVSNET
                     AMYWYWFSGAQDDA"
     gene            2282..3070
                     /gene="aadA1"
                     /locus_tag="OO357_004424"
     CDS             2282..3070
                     /gene="aadA1"
                     /locus_tag="OO357_004424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5889076.1"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          862 a          696 c          759 g          813 t
ORIGIN      
        1 gtatgacaag tgacacgctt gctaacgatg cctgcttttt gtacggctgc cttcaatgcc
       61 tttcgcgcaa cggagtcatg cagatgatgg cggcataatt tgccgttata cgggtggttg
      121 cagagcgtgc tggagggaaa gacaaacatc cacgccgctt gtcgataagc agaagggtat
      181 ttgtgatcta aagcaaaagg cagcgatggc cctacgcctt gtaagttgtc gtcttgctga
      241 ataagccgcg cttgctcaat gagttatttt attgctggga ttaggcgcgt gggcagtagg
      301 ctgtttctgc ttttcccacc cttaccgtca tgcacagtga tgcagccatt atcaaaatca
      361 aaatctttaa cccgcaaacg caagcattca ttaatgcgca aacctgcacc atacagcagc
      421 gtaaaaataa cttggttgcg agtatccata acctgcaaaa tgcgttgcac ttcatttgca
      481 gagataacag agggtagccg tctaggcttg cttgcaggga tataatcaat atcgcccaac
      541 ggctgttgta aaaacctgtt gtacaaaaaa gctagggcat ttaaagcgat tttctgcgtg
      601 tttatggcta catgtctgct gtttgctaag ctggataaaa acagcctgac ctcttcactg
      661 cccatggtct gaggatgacg ttttttgtga aacagaataa aacgcttaat ccagtgcagg
      721 taagtttttt cagttttcag cgcataacct ttttgtcgca tatccgtgcg tatagaattt
      781 aaaaatggac tgttagacat aaaacgctcc ttgtcttgca actgtctgcc tatacagcct
      841 attctagctg ggatttaaaa aagtgcctgt tttttacgcc tagagatgct tgtttaccgg
      901 tagagtttta atttaatgct aaataaatta aaatgttatg agttctttgg gtgagataat
      961 gtgcatcgtg caagcaggat agacggcatg cacgatttgt aataacagag tgtcttgtat
     1021 ttttaaagaa agtctattta atacaagtga ttatattaat taacggtaag catcagcggg
     1081 tgacaaaacg agcatgctta ctaataaaat gttaacctct gaggaagaat tgtgaaacta
     1141 tcactaatgg tagctatatc gaagaatgga gttatcggga atggccctga tattccatgg
     1201 agtgccaaag gtgaacagct cctgtttaaa gctattacct ataaccaatg gctgttggtt
     1261 ggacgcaaga cttttgaatc aatgggagca ttacccaacc gaaagtatgc ggtcgtaaca
     1321 cgttcaagtt ttacatctga caatgagaac gtattgatct ttccatcaat taaagatgct
     1381 ttaaccaacc taaagaaaat aacggatcat gtcattgttt caggtggtgg ggagatatac
     1441 aaaagcctga tcgatcaagt agatacacta catatatcta caatagacat cgagccggaa
     1501 ggtgatgttt actttcctga aatccccagc aattttaggc cagtttttac ccaagacttc
     1561 gcctctaaca taaattatag ttaccaaatc tggcaaaagg gttaacaagt ggcagcaacg
     1621 gattcgcaaa cctgtcacgc cttttgtacc aaaagccgcg ccaggtttgc gatccgctgt
     1681 gccaggcgtt aggcgtcata tgaagatttc ggtgatccct gagcaggtgg cggaaacatt
     1741 ggatgctgag aaccatttca ttgttcgtga agtgttcgat gtgcacctat ccgaccaagg
     1801 ctttgaacta tctaccagaa gtgtgagccc ctaccggaag gattacatct cggatgatga
     1861 ctctgatgaa gactctgctt gctatggcgc attcatcgac caagagcttg tcgggaagat
     1921 tgaactcaac tcaacatgga acgatctagc ctctatcgaa cacattgttg tgtcgcacac
     1981 gcaccgaggc aaaggagtcg cgcacagtct catcgaattt gcgaaaaagt gggcactaag
     2041 cagacagctc cttggcatac gattagagac acaaacgaac aatgtacctg cctgcaattt
     2101 gtacgcaaaa tgtggcttta ctctcggcgg cattgacctg ttcacgtata aaactagacc
     2161 tcaagtctcg aacgaaacag cgatgtactg gtactggttc tcgggagcac aggatgacgc
     2221 ctaacaattc attcaagccg acaccgcttc gcggcgcggc ttaattcagg agttaaacat
     2281 catgagggaa gcggtgatcg ccgaagtatc gactcaacta tcagaggtag ttggcgtcat
     2341 cgagcgccat ctcgaaccga cgttgctggc cgtacatttg tacggctccg cagtggatgg
     2401 cggcctgaag ccacacagtg atattgattt gctggttacg gtgaccgtaa ggcttgatga
     2461 aacaacgcgg cgagctttga tcaacgacct tttggaaact tcggcttccc ctggagagag
     2521 cgagattctc cgcgctgtag aagtcaccat tgttgtgcac gacgacatca ttccgtggcg
     2581 ttatccagct aagcgcgaac tgcaatttgg agaatggcag cgcaatgaca ttcttgcagg
     2641 tatcttcgag ccagccacga tcgacattga tctggctatc ttgctgacaa aagcaagaga
     2701 acatagcgtt gccttggtag gtccagcggc ggaggaactc tttgatccgg ttcctgaaca
     2761 ggatctattt gaggcgctaa atgaaacctt aacgctatgg aactcgccgc ccgactgggc
     2821 tggcgatgag cgaaatgtag tgcttacgtt gtcccgcatt tggtacagcg cagtaaccgg
     2881 caaaatcgcg ccgaaggatg tcgctgccga ctgggcaatg gagcgcctgc cggcccagta
     2941 tcagcccgtc atacttgaag ctaggcaggc ttatcttgga caagaagatc gcttggcctc
     3001 gcgcgcagat cagttggaag aatttgttca ctacgtgaaa ggcgagatca ccaaggtagt
     3061 cggcaaataa tgtctaacaa ttcgttcaag ccgacgccgc ttcgcggcgc ggcttaactc
     3121 aagcgttaga
//
LOCUS       DAJTRC010000412         1264 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.guided.281, whole genome shotgun sequence.
ACCESSION   DAJTRC010000412 DAJTRC010000000
VERSION     DAJTRC010000412.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.guided.281"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..300
                     /locus_tag="OO357_004425"
     CDS             <1..300
                     /locus_tag="OO357_004425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HCR5889077.1"
                     /translation="FLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGAGFT
                     ANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            413..1204
                     /gene="aadA1"
                     /locus_tag="OO357_004426"
                     /pseudo
     CDS             413..1204
                     /gene="aadA1"
                     /locus_tag="OO357_004426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
BASE COUNT          360 a          301 c          311 g          292 t
ORIGIN      
        1 ttcctgcgta aaattattaa tcacaatctc ccagttaaaa actcagccat agaaaacacc
       61 atagagaaca tgtatctaca agatctggat aatagtacaa aactgtatgg gaaaactggt
      121 gcaggattca cagcaaatag aaccttacaa aacggatggt ttgaagggtt tattataagc
      181 aaatcaggac ataaatatgt ttttgtgtcc gcacttacag gaaacttggg gtcgaattta
      241 acatcaagca taaaagccaa gaaaaatgcg atcaccattc taaacacact aaatttataa
      301 aaaatctaat ggcaaaatcg cccaaccctt caatcaagtc gggacggcca aaagcaagct
      361 tttggctccc ctcgctggcg ctcggcgccc cttatttcaa acgttaaaca tcatgaggga
      421 agtggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
      481 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
      541 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
      601 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
      661 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttaaccagc
      721 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
      781 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
      841 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
      901 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
      961 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     1021 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     1081 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     1141 agatcagttg gaagaatttg ttcactacgt gaaaggcgag atcaccaagg tagtcggcaa
     1201 ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt
     1261 taga
//
LOCUS       DAJTRC010000413         1264 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.guided.282, whole genome shotgun sequence.
ACCESSION   DAJTRC010000413 DAJTRC010000000
VERSION     DAJTRC010000413.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1264)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1264)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1264
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.guided.282"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..300
                     /locus_tag="OO357_004427"
     CDS             <1..300
                     /locus_tag="OO357_004427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HCR5889078.1"
                     /translation="FLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGAGFT
                     ANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            413..1204
                     /gene="aadA1"
                     /locus_tag="OO357_004428"
     CDS             413..1204
                     /gene="aadA1"
                     /locus_tag="OO357_004428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5889079.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          359 a          301 c          311 g          293 t
ORIGIN      
        1 ttcctgcgta aaattattaa tcacaatctc ccagttaaaa actcagccat agaaaacacc
       61 atagagaaca tgtatctaca agatctggat aatagtacaa aactgtatgg gaaaactggt
      121 gcaggattca cagcaaatag aaccttacaa aacggatggt ttgaagggtt tattataagc
      181 aaatcaggac ataaatatgt ttttgtgtcc gcacttacag gaaacttggg gtcgaattta
      241 acatcaagca taaaagccaa gaaaaatgcg atcaccattc taaacacact aaatttataa
      301 aaaatctaat ggcaaaatcg cccaaccctt caatcaagtc gggacggcca aaagcaagct
      361 tttggctccc ctcgctggcg ctcggcgccc cttatttcaa acgttaaaca tcatgaggga
      421 agtggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
      481 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
      541 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
      601 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
      661 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
      721 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
      781 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
      841 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
      901 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
      961 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     1021 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     1081 catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg cctcgcgcgc
     1141 agatcagttg gaagaatttg ttcactacgt gaaaggcgag atcaccaagg tagtcggcaa
     1201 ataatgtcta acaattcgtt caagccgacg ccgcttcgcg gcgcggctta actcaagcgt
     1261 taga
//
LOCUS       DAJTRC010000414         1261 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.guided.283, whole genome shotgun sequence.
ACCESSION   DAJTRC010000414 DAJTRC010000000
VERSION     DAJTRC010000414.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.guided.283"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..300
                     /locus_tag="OO357_004429"
     CDS             <1..300
                     /locus_tag="OO357_004429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HCR5889080.1"
                     /translation="FLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGAGFT
                     ANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            413..1201
                     /locus_tag="OO357_004430"
                     /pseudo
     CDS             413..1201
                     /locus_tag="OO357_004430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="AadA family aminoglycoside
                     3''-O-nucleotidyltransferase"
BASE COUNT          357 a          301 c          311 g          292 t
ORIGIN      
        1 ttcctgcgta aaattattaa tcacaatctc ccagttaaaa actcagccat agaaaacacc
       61 atagagaaca tgtatctaca agatctggat aatagtacaa aactgtatgg gaaaactggt
      121 gcaggattca cagcaaatag aaccttacaa aacggatggt ttgaagggtt tattataagc
      181 aaatcaggac ataaatatgt ttttgtgtcc gcacttacag gaaacttggg gtcgaattta
      241 acatcaagca taaaagccaa gaaaaatgcg atcaccattc taaacacact aaatttataa
      301 aaaatctaat ggcaaaatcg cccaaccctt caatcaagtc gggacggcca aaagcaagct
      361 tttggctccc ctcgctggcg ctcggcgccc cttatttcaa acgttaaaca tcatgaggga
      421 agtggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
      481 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
      541 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
      601 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
      661 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttaaccagc
      721 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
      781 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
      841 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
      901 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
      961 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     1021 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     1081 catacttgaa gctaggcagg cttatcttgg acaagaagat cgcttggcct cgcgcgcaga
     1141 tcagttggaa gaatttgttc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     1201 atgtctaaca attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag
     1261 a
//
LOCUS       DAJTRC010000415         1261 bp    DNA     linear   BCT 07-NOV-2022
DEFINITION  TPA_asm: Shigella flexneri isolate 503010
            SAMEA8900174-rid15819683.guided.284, whole genome shotgun sequence.
ACCESSION   DAJTRC010000415 DAJTRC010000000
VERSION     DAJTRC010000415.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMEA8900174
            Sequence Read Archive: ERR6004893
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Shigella flexneri
  ORGANISM  Shigella flexneri
            Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
            Enterobacteriaceae; Shigella.
REFERENCE   1  (bases 1 to 1261)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1261)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (05-NOV-2022) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 05-NOV-2022
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT001477807.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT001477807.1
            Genome Coverage       :: 31x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 11/05/2022 23:33:45
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 4,432
            CDSs (total)                      :: 4,362
            Genes (coding)                    :: 3,976
            CDSs (with protein)               :: 3,976
            Genes (RNA)                       :: 70
            rRNAs                             :: 1, 2, 1 (5S, 16S, 23S)
            complete rRNAs                    :: 1 (23S)
            partial rRNAs                     :: 1, 2 (5S, 16S)
            tRNAs                             :: 59
            ncRNAs                            :: 7
            Pseudo Genes (total)              :: 386
            CDSs (without protein)            :: 386
            Pseudo Genes (ambiguous residues) :: 0 of 386
            Pseudo Genes (frameshifted)       :: 173 of 386
            Pseudo Genes (incomplete)         :: 97 of 386
            Pseudo Genes (internal stop)      :: 154 of 386
            Pseudo Genes (multiple problems)  :: 37 of 386
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1261
                     /organism="Shigella flexneri"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMEA8900174-rid15819683.guided.284"
                     /serovar="S. flexneri 2a"
                     /isolate="503010"
                     /isolation_source="stool"
                     /host="Homo sapiens"
                     /db_xref="taxon:623"
                     /country="India"
                     /collection_date="2009-02-08"
     gene            <1..300
                     /locus_tag="OO357_004431"
     CDS             <1..300
                     /locus_tag="OO357_004431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D beta-lactamase"
                     /protein_id="HCR5889081.1"
                     /translation="FLRKIINHNLPVKNSAIENTIENMYLQDLDNSTKLYGKTGAGFT
                     ANRTLQNGWFEGFIISKSGHKYVFVSALTGNLGSNLTSSIKAKKNAITILNTLNL"
     gene            413..1201
                     /gene="aadA1"
                     /locus_tag="OO357_004432"
     CDS             413..1201
                     /gene="aadA1"
                     /locus_tag="OO357_004432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014325834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ANT(3'')-Ia family aminoglycoside
                     nucleotidyltransferase AadA1"
                     /protein_id="HCR5889082.1"
                     /translation="MREVVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKP
                     HSDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYP
                     AKRELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQ
                     DLFEALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPA
                     QYQPVILEARQAYLGQEDRLASRADQLEEFVHYVKGEITKVVGK"
BASE COUNT          356 a          301 c          311 g          293 t
ORIGIN      
        1 ttcctgcgta aaattattaa tcacaatctc ccagttaaaa actcagccat agaaaacacc
       61 atagagaaca tgtatctaca agatctggat aatagtacaa aactgtatgg gaaaactggt
      121 gcaggattca cagcaaatag aaccttacaa aacggatggt ttgaagggtt tattataagc
      181 aaatcaggac ataaatatgt ttttgtgtcc gcacttacag gaaacttggg gtcgaattta
      241 acatcaagca taaaagccaa gaaaaatgcg atcaccattc taaacacact aaatttataa
      301 aaaatctaat ggcaaaatcg cccaaccctt caatcaagtc gggacggcca aaagcaagct
      361 tttggctccc ctcgctggcg ctcggcgccc cttatttcaa acgttaaaca tcatgaggga
      421 agtggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca tcgagcgcca
      481 tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg gcggcctgaa
      541 gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg aaacaacgcg
      601 gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga gcgagattct
      661 ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc gttatccagc
      721 taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag gtatcttcga
      781 gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag aacatagcgt
      841 tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac aggatctatt
      901 tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg ctggcgatga
      961 gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg gcaaaatcgc
     1021 gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt atcagcccgt
     1081 catacttgaa gctaggcagg cttatcttgg acaagaagat cgcttggcct cgcgcgcaga
     1141 tcagttggaa gaatttgttc actacgtgaa aggcgagatc accaaggtag tcggcaaata
     1201 atgtctaaca attcgttcaa gccgacgccg cttcgcggcg cggcttaact caagcgttag
     1261 a
//
